LOCUS       DAPGOF010000001        70038 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.001, whole genome shotgun sequence.
ACCESSION   DAPGOF010000001 DAPGOF010000000
VERSION     DAPGOF010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 70038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70038
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.001"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..500
                     /locus_tag="RHV13_000001"
     CDS             <1..500
                     /locus_tag="RHV13_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDV1239569.1"
                     /translation="LLPVVMLASFTLAGCQSNADDHAADVYQTDQLNTKQETKTVNII
                     SILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVGGGAVGAA
                     AGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNETRIQPNTK
                     CPEKS"
     gene            516..1055
                     /locus_tag="RHV13_000002"
     CDS             516..1055
                     /locus_tag="RHV13_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HDV1239570.1"
                     /translation="MKKVFLCAILASLSYPATASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1148..2725)
                     /gene="guaA"
                     /locus_tag="RHV13_000003"
     CDS             complement(1148..2725)
                     /gene="guaA"
                     /locus_tag="RHV13_000003"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDV1239571.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFITVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2794..4260)
                     /gene="guaB"
                     /locus_tag="RHV13_000004"
     CDS             complement(2794..4260)
                     /gene="guaB"
                     /locus_tag="RHV13_000004"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDV1239572.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4422..5792
                     /gene="xseA"
                     /locus_tag="RHV13_000005"
     CDS             4422..5792
                     /gene="xseA"
                     /locus_tag="RHV13_000005"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDV1239573.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQRKAKLQAEGLFDLQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGKVLKKVKQVKAGEMLTTRLEDGWVESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5789..6004)
                     /locus_tag="RHV13_000006"
     CDS             complement(5789..6004)
                     /locus_tag="RHV13_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDV1239574.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6074..7546)
                     /gene="der"
                     /locus_tag="RHV13_000007"
     CDS             complement(6074..7546)
                     /gene="der"
                     /locus_tag="RHV13_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDV1239575.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7664..8842)
                     /gene="bamB"
                     /locus_tag="RHV13_000008"
     CDS             complement(7664..8842)
                     /gene="bamB"
                     /locus_tag="RHV13_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HDV1239576.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8853..9473)
                     /gene="yfgM"
                     /locus_tag="RHV13_000009"
     CDS             complement(8853..9473)
                     /gene="yfgM"
                     /locus_tag="RHV13_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HDV1239577.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9491..10765)
                     /gene="hisS"
                     /locus_tag="RHV13_000010"
     CDS             complement(9491..10765)
                     /gene="hisS"
                     /locus_tag="RHV13_000010"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDV1239578.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIVYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10876..11994)
                     /gene="ispG"
                     /locus_tag="RHV13_000011"
                     /gene_synonym="gcpE"
     CDS             complement(10876..11994)
                     /gene="ispG"
                     /locus_tag="RHV13_000011"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HDV1239579.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRVKASQLDEARRIDVQQVEK"
     gene            complement(12021..13034)
                     /gene="rodZ"
                     /locus_tag="RHV13_000012"
     CDS             complement(12021..13034)
                     /gene="rodZ"
                     /locus_tag="RHV13_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HDV1239580.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTATTTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13319..14473)
                     /gene="trmG/rlmN"
                     /locus_tag="RHV13_000013"
     CDS             complement(13319..14473)
                     /gene="trmG/rlmN"
                     /locus_tag="RHV13_000013"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HDV1239581.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14623..15054)
                     /gene="ndk"
                     /locus_tag="RHV13_000014"
     CDS             complement(14623..15054)
                     /gene="ndk"
                     /locus_tag="RHV13_000014"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDV1239582.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGKGEVCPRTR"
     gene            complement(15203..17515)
                     /gene="pbpC"
                     /locus_tag="RHV13_000015"
     CDS             complement(15203..17515)
                     /gene="pbpC"
                     /locus_tag="RHV13_000015"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HDV1239583.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAKPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAKLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(17516..22477)
                     /gene="yfhM"
                     /locus_tag="RHV13_000016"
     CDS             complement(17516..22477)
                     /gene="yfhM"
                     /locus_tag="RHV13_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HDV1239584.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGLRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADEIGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAVPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVSTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTSYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQLATLQVRNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            22684..23529
                     /gene="sseA"
                     /locus_tag="RHV13_000017"
     CDS             22684..23529
                     /gene="sseA"
                     /locus_tag="RHV13_000017"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HDV1239585.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPLK"
     gene            complement(24348..25124)
                     /gene="sseB"
                     /locus_tag="RHV13_000018"
     CDS             complement(24348..25124)
                     /gene="sseB"
                     /locus_tag="RHV13_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HDV1239586.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(25266..26549)
                     /gene="pepB"
                     /locus_tag="RHV13_000019"
     CDS             complement(25266..26549)
                     /gene="pepB"
                     /locus_tag="RHV13_000019"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HDV1239587.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(26727..26927)
                     /gene="iscX"
                     /locus_tag="RHV13_000020"
     CDS             complement(26727..26927)
                     /gene="iscX"
                     /locus_tag="RHV13_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HDV1239588.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(26939..27274)
                     /gene="fdx"
                     /locus_tag="RHV13_000021"
     CDS             complement(26939..27274)
                     /gene="fdx"
                     /locus_tag="RHV13_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HDV1239589.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(27276..29126)
                     /gene="hscA"
                     /locus_tag="RHV13_000022"
     CDS             complement(27276..29126)
                     /gene="hscA"
                     /locus_tag="RHV13_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HDV1239590.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(29143..29658)
                     /gene="hscB"
                     /locus_tag="RHV13_000023"
     CDS             complement(29143..29658)
                     /gene="hscB"
                     /locus_tag="RHV13_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HDV1239591.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(29754..30077)
                     /gene="iscA"
                     /locus_tag="RHV13_000024"
     CDS             complement(29754..30077)
                     /gene="iscA"
                     /locus_tag="RHV13_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HDV1239592.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(30094..30480)
                     /gene="iscU"
                     /locus_tag="RHV13_000025"
     CDS             complement(30094..30480)
                     /gene="iscU"
                     /locus_tag="RHV13_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HDV1239593.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(30508..31722)
                     /gene="iscS"
                     /locus_tag="RHV13_000026"
     CDS             complement(30508..31722)
                     /gene="iscS"
                     /locus_tag="RHV13_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDV1239594.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(31834..32322)
                     /gene="iscR"
                     /locus_tag="RHV13_000027"
     CDS             complement(31834..32322)
                     /gene="iscR"
                     /locus_tag="RHV13_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HDV1239595.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(32683..33423)
                     /gene="trmJ"
                     /locus_tag="RHV13_000028"
     CDS             complement(32683..33423)
                     /gene="trmJ"
                     /locus_tag="RHV13_000028"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HDV1239596.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            33542..34345
                     /gene="suhB"
                     /locus_tag="RHV13_000029"
     CDS             33542..34345
                     /gene="suhB"
                     /locus_tag="RHV13_000029"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HDV1239597.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            34490..35344
                     /locus_tag="RHV13_000030"
     CDS             34490..35344
                     /locus_tag="RHV13_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV1239598.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            35535..36815
                     /gene="csiE"
                     /locus_tag="RHV13_000031"
     CDS             35535..36815
                     /gene="csiE"
                     /locus_tag="RHV13_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HDV1239599.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATTATFSELNGVDD
                     DIASLDISETGQEILRYHQLTLTAGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSHFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHISVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(36807..37946)
                     /locus_tag="RHV13_000032"
     CDS             complement(36807..37946)
                     /locus_tag="RHV13_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HDV1239600.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(38106..38996)
                     /gene="hcaR"
                     /locus_tag="RHV13_000033"
     CDS             complement(38106..38996)
                     /gene="hcaR"
                     /locus_tag="RHV13_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HDV1239601.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            39132..40493
                     /locus_tag="RHV13_000034"
     CDS             39132..40493
                     /locus_tag="RHV13_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HDV1239602.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            40490..41008
                     /locus_tag="RHV13_000035"
     CDS             40490..41008
                     /locus_tag="RHV13_000035"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HDV1239603.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            41008..41328
                     /locus_tag="RHV13_000036"
     CDS             41008..41328
                     /locus_tag="RHV13_000036"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HDV1239604.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            41325..42137
                     /gene="hcaB"
                     /locus_tag="RHV13_000037"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             41325..42137
                     /gene="hcaB"
                     /locus_tag="RHV13_000037"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HDV1239605.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            42147..43349
                     /locus_tag="RHV13_000038"
     CDS             42147..43349
                     /locus_tag="RHV13_000038"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HDV1239606.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIIGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANDQLAREANLDTVNGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRSIRKWIQSGKTFDAKQLTD
                     ENIALKSL"
     gene            43446..43868
                     /locus_tag="RHV13_000039"
     CDS             43446..43868
                     /locus_tag="RHV13_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDV1239607.1"
                     /translation="MNSLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(43916..44788)
                     /locus_tag="RHV13_000040"
     CDS             complement(43916..44788)
                     /locus_tag="RHV13_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HDV1239608.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIIETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(44800..45894)
                     /locus_tag="RHV13_000041"
     CDS             complement(44800..45894)
                     /locus_tag="RHV13_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDV1239609.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YKDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(45927..46925)
                     /locus_tag="RHV13_000042"
     CDS             complement(45927..46925)
                     /locus_tag="RHV13_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV1239610.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(46949..48460)
                     /locus_tag="RHV13_000043"
     CDS             complement(46949..48460)
                     /locus_tag="RHV13_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDV1239611.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGEIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAHKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHAPVNVDELMSAILSVH"
     gene            complement(48483..49466)
                     /locus_tag="RHV13_000044"
     CDS             complement(48483..49466)
                     /locus_tag="RHV13_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDV1239612.1"
                     /translation="MPTKIRTTRNLLLMATLLGSALFARAAEKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASGAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKARVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(49563..52844)
                     /locus_tag="RHV13_000045"
     CDS             complement(49563..52844)
                     /locus_tag="RHV13_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HDV1239613.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHGDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCD
                     ALLDDAVALTPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGCQDNARL
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDARQPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEATRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQIAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            52962..54155
                     /locus_tag="RHV13_000046"
     CDS             52962..54155
                     /locus_tag="RHV13_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDV1239614.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGMSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(54353..55606)
                     /gene="glyA"
                     /locus_tag="RHV13_000047"
     CDS             complement(54353..55606)
                     /gene="glyA"
                     /locus_tag="RHV13_000047"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDV1239615.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            55934..57124
                     /gene="hmpA"
                     /locus_tag="RHV13_000048"
     CDS             55934..57124
                     /gene="hmpA"
                     /locus_tag="RHV13_000048"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HDV1239616.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(57169..57507)
                     /gene="glnB"
                     /locus_tag="RHV13_000049"
     CDS             complement(57169..57507)
                     /gene="glnB"
                     /locus_tag="RHV13_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HDV1239617.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(57568..58902)
                     /gene="glrR"
                     /locus_tag="RHV13_000050"
     CDS             complement(57568..58902)
                     /gene="glrR"
                     /locus_tag="RHV13_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HDV1239618.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQKGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDNDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(58892..59605)
                     /gene="qseG"
                     /locus_tag="RHV13_000051"
     CDS             complement(58892..59605)
                     /gene="qseG"
                     /locus_tag="RHV13_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HDV1239619.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHEGEKPAPSTHEVTPDEP"
     gene            complement(59770..61197)
                     /gene="qseE"
                     /locus_tag="RHV13_000052"
     CDS             complement(59770..61197)
                     /gene="qseE"
                     /locus_tag="RHV13_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HDV1239620.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELDNVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(61773..65660)
                     /gene="purL"
                     /locus_tag="RHV13_000053"
                     /gene_synonym="purI"
     CDS             complement(61773..65660)
                     /gene="purL"
                     /locus_tag="RHV13_000053"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HDV1239621.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIATLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            65918..67474
                     /gene="mltF"
                     /locus_tag="RHV13_000054"
     CDS             65918..67474
                     /gene="mltF"
                     /locus_tag="RHV13_000054"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HDV1239622.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEEVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(67471..68007)
                     /gene="tadA"
                     /locus_tag="RHV13_000055"
     CDS             complement(67471..68007)
                     /gene="tadA"
                     /locus_tag="RHV13_000055"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HDV1239623.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHAMTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(68032..68667)
                     /gene="yfhb"
                     /locus_tag="RHV13_000056"
     CDS             complement(68032..68667)
                     /gene="yfhb"
                     /locus_tag="RHV13_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HDV1239624.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHNEARLQALQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            68876..69724
                     /locus_tag="RHV13_000057"
     CDS             68876..69724
                     /locus_tag="RHV13_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDV1239625.1"
                     /translation="MNGLLRIRQRYQGFAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTGAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            69780..>70038
                     /locus_tag="RHV13_000058"
     CDS             69780..>70038
                     /locus_tag="RHV13_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HDV1239626.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPQHVETEEQLWDKFVLMHHADKI"
BASE COUNT        16639 a        19299 c        17660 g        16440 t
ORIGIN      
        1 gtcttctgcc tgtggtaatg ttagcgtcat tcactctggc aggatgccag tcaaatgctg
       61 acgatcatgc cgccgatgtt tatcaaaccg atcaactgaa taccaaacaa gaaactaaaa
      121 ccgttaatat tatttccatt cttcccgcaa aagttgccgt agacaactcc caaaataaac
      181 ggaacgcaca agccttcggc gcgcttattg gcgcagtcgc tggcggtgtt atcggccaca
      241 acgtcgggtc tggcagcaat tccggaacga cggcaggtgc agttggcggc ggagctgtag
      301 gcgcggcagc gggttctatg gtgaatgata aaaccttagt ggaaggtgtt tctttaacgt
      361 ataaggaagg caccaaagtg tatacctcta ctcaggtggg taaagagtgc cagtttacga
      421 caggtttagc cgttgttatt accacgacgt ataacgaaac gcgtattcag ccaaatacca
      481 aatgtcctga aaagagctaa taatcaggag gagtcatgaa gaaagttttt ctttgcgcca
      541 tcttagcctc cttaagctat ccggctaccg cctcatcatt gcaggatcaa ctctctgctg
      601 tcgcagaagc ggaacagcaa ggtaaaaatg aagagcaaag gcagcatgac gaatgggtcg
      661 cggagcgcaa cagggaaatc cagcaagaga agcaacgtcg cgcaaatgcc caggccgccg
      721 ctaacaaaag agcggcaacg gcagcggcaa ataagaaagc tcgtcaggat aaactggacg
      781 ccgaagccac tgcggacaaa aaacgcgatc aaagttatga agatgagcta cgcagcttag
      841 agattcagaa acaaaaactg gcgctggcga aagaagaagc ccgcgttaag cgcgaaaacg
      901 aatttatcga tcaggaactg aagcacaaag ctgcgcaaac cgatgtggtg caatctgaag
      961 ctgacgccaa cagaaatatg actgaaggcg gtcgcgatct gatgaaaagc gtgggcaaag
     1021 cagaagagaa caaatcggac agctggttta attaatcgat gttagtaact tcaagcctat
     1081 aattcttgaa gataaaaaac cctctgtagt aacagagggt tttgttcatt catagtgcag
     1141 ggtcaaatca ttcccactca atggtagctg gcggcttgcc gctgatgtca tacaccacgc
     1201 gggaaatacc gttcacttca ttgataatgc ggttggaaac gcgaccgagg aaatcgtacg
     1261 gcagatgcgc ccagtgtgcg gtcataaagt cgatggtttc gacagcacgc agagagacaa
     1321 cccagtcata cttacgacca tcgcccatta cgccaacgga acgtactggc aggaagacgg
     1381 tgaacgcctg gctgactttg tcgtacaggt ccgctttacg cagttcttca atgaagatgg
     1441 cgtcagcacg gcgcagcagg tcacagtact ctttcttcac ttcacccaga acacgaacgc
     1501 caaggcctgg tcccgggaac gggtgacggt acagcatgtc gtacggcagg cccagctcca
     1561 gaccaatctt acgcacttcg tctttgaaca gctctttcag cggttcaacc aggcccatct
     1621 tcatctcttt cggcaggccg cccacgttgt ggtgagattt gatgacgtgt gctttaccgg
     1681 ttgcagacgc cgcagattcg ataacgtcag ggtagatggt gccctgcgcc agccacttca
     1741 cgtcttccag tttcagcgct tcttcatcga aaacttcaac gaaaacgcga ccgatgattt
     1801 tacgttttgc ttccggatcg ttttcaccag ccagcgctga caggaagcga tcttctgccg
     1861 gtacgtgaac aatgttaaga ccaaagtgat cgccaaacat atccagaacc tgctctgctt
     1921 cgttgagacg cagcaggccg ttgtcgacga atacgcaagt caggttttta ccgatagcgc
     1981 ggtgcagcag cattgcggtt acggaggaat ccacaccacc agagaggccg aggatgactt
     2041 tatcgtcgcc tacctgttcg cggatgcgag ctacagcatc gtcgataatt ttcgctggcg
     2101 tccacagggc ttcacactgg cagatatcac gcacaaaacg ctccagcatg cgcataccct
     2161 ggcgggtgtg agtcacttcc gggtggaact gtacgccata gaagcgtttt tcttcgttag
     2221 ccataatggc aaacgggcag ctttcggtgc tggctacggt gatgaagtcg gacgggatag
     2281 cggtaacttt atcgccgtgg ctcatccaga catcgagcag cggtttaccg tctgcggtca
     2341 gcgcatcttc gataccgcga accagtgcgc tgtcgtttac gacttcaacc tgcgcgtagc
     2401 caaattcacg ttcgttagag gcttcaacgt gaccgcccaa ctgcattgcc atggtctgca
     2461 tgccatagca aacgccgaat accggtacgc ctgcttcaaa gacatactgc ggcgcacgcg
     2521 ggctgttttc ttcagtagta ctttccgggc cgccggaaag aataatgccg cttggattga
     2581 agtcacgaat ttgtgcttct gtcacatccc acgcccacag ttcgcagtaa acacccagct
     2641 cacgcacgcg gcgcgcaacc agttgagtgt actgagaacc gaagtccaga atgaggatgc
     2701 gatgcttatg aatgttttcc gtcattgacg cttattccga ggcaagtgaa acagataata
     2761 taaatcgccc gacatgaagt cgggcgaaga gaatcaggag cccagacggt agttcgggga
     2821 ctctttagta atggtcacgt cgtgaacgtg gctttcctga atgcccgcac cgctgatacg
     2881 tacaaactcc gctttagtac gcagttcgtc gatagtacca cagccggtca gacccataca
     2941 ggagcgcagg ccgcccatct gctggtgaat gatctctttc aggcgacctt tataagctac
     3001 gcgaccttcg ataccttccg gcaccagttt gtcggcagcg ttatcgctct ggaaataacg
     3061 gtcagaggaa cctttggaca tcgcgcccag ggaacccata ccacggtaag atttgtaaga
     3121 acggccctgg tagagttcga tttcacccgg agattcttca gtacccgcca gcatggaacc
     3181 taccatcacc gcgcttgcgc cagcggcgat agctttggcg atgtcgccgg agaagcgaat
     3241 accaccatct gcgataaccg gaataccggt gccttccagg gcttcaactg cgtcagcaac
     3301 ggcagtgatc tgcggaacac ctacgccagt aacgatacga gtcgtacaga tagaaccagg
     3361 gccgataccg acttttaccg cgctgcaacc agcttctgcc agagcacgtg cacctgcagc
     3421 tgttgccacg ttgccgccga tgatttgcag atccggatat ttagcacggg tttcacggat
     3481 acgctgcaaa acgccttctg agtgaccgtg ggaggagtcg atcagcagaa cgtcaacgcc
     3541 agcggcaacc agcgcgtcaa cacgctcttc gttaccagca cctgcgccaa ctgcagcacc
     3601 aacacgcaga cggccttgct cgtctttaca ggcgttcggt ttacgttccg ctttctggaa
     3661 atctttcacg gtgatcatgc cgatcaggtg gaattcgtca tcaaccacca gcgctttttc
     3721 aacgcgtttt tcgtgcattt ttgccagcac cacttcacgg gcttcacctt cacgcacggt
     3781 gaccagacgc tctttcggcg tcatgtaaac gctaaccggc tggttcaggt cggtaacaaa
     3841 acgcacgtca cgaccggtga taatacccac cagttcgttt tcttcggtaa cgaccggata
     3901 gcccgcaaaa ccgttacgct cggtcagttc tttcacttcg cgcagcgtcg tggttggcag
     3961 aacagtctgc ggatcagtca ccacaccaga ttcgtgtttt ttcacacggc gaacttcttc
     4021 tgcctggcgt tcaatggaca tgtttttgtg gataaagccg ataccgcctt cctgagccag
     4081 agcaatagcc aggcgcgctt ccgttacggt atccattgct gcggaaagca tagggatatt
     4141 cagacgaata gttttcgtca gctgggtgct gaggtcagca gtattcggca gaacggtaga
     4201 atgagcagga acgaggagaa cgtcgtcaaa cgtcagagct tctttagcga tacgtagcat
     4261 gggcaatatc tcgaccagag tggttaataa atattgccgc ggcattatac agagcgtaac
     4321 cgattgcatc tacccctttt tgcaaaaaat gcttgctatc cccgaagggc gggttactat
     4381 cgactgaata acctgctgat ttagaatttg atctcgctca catgttacct tctcaatccc
     4441 ctgcaatttt taccgttagt cgcctgaatc aaacggttcg tctgctgctt gagcatgaga
     4501 tgggacaggt ttggatcagc ggcgaaatct ctaatttcac acaaccggct tccggtcact
     4561 ggtactttac actcaaagac gacaccgccc aggtacgctg cgcgatgttc cgcaacagca
     4621 accgtcgggt gaccttccgt ccacagcatg ggcaacaagt tttagttcgc gccaatatta
     4681 cgctctacga gccgcgcggc gactaccaga taatcgttga gagtatgcag ccggccggtg
     4741 aagggctgct gcaacagaag tacgaacagc gcaaagcgaa gttgcaggct gaaggattgt
     4801 tcgatctgca atacaaaaaa ccacttccct cccctgcgca ttgcgttggt gtgatcacct
     4861 caaaaaccgg tgctgcgcta catgatattt tgcatgtgtt aaaacgtcgc gatccttctc
     4921 taccggtgat catctacccc accgccgttc agggcgatga cgcgccgggg caaattgttc
     4981 gcgccattga actggcgaat cagcgcaatg agtgcgacgt gttgatcgtt gggcgcggcg
     5041 gcggttcgct ggaagattta tggagtttta acgacgaacg cgtagcgcgg gcgatttttg
     5101 ccagccgcat tccggtcgtc agcgccgtcg ggcatgagac ggatgtgacc attgccgatt
     5161 ttgttgccga tctgcgtgca ccaacaccgt cggctgccgc tgaagtagtg agccgtaatc
     5221 agcaagagtt actgcgccag gtgcaatcgg cccgtcaacg gctggagatg gcgatggatt
     5281 attatctcgc caaccgtacg cgtcgcttta cgcagattca tcatcgcttg cagcagcagc
     5341 atccgcagct ccggctggca cgccagcaaa ccatgcttga acgcctgcaa aaacggatga
     5401 gctttgcgct ggaaaatcag cttaagcgtg ccgggcaaca gcagcagcga ttaacacagc
     5461 ggctgaatca gcaaaatcca caaccgaaga ttcatcgcgc gcaaacgcgc attcagcaac
     5521 tggaatatcg tttagcagaa accctgcgcg cacagcttag cgccacgcgt gaacgtttcg
     5581 gtaatgcagt aacgcacctc gaagccgtaa gcccactgtc aacgctggcg cgtggataca
     5641 gcgttactac tgctactgac ggcaaggtat tgaaaaaagt gaagcaggtt aaagcgggtg
     5701 aaatgctaac cacacgtctg gaagacggct gggtagaaag tgaagtaaaa aacatccagc
     5761 cggtaaaaaa atcgcgtaaa aaggtgcatt aagccagcac gaattcaacg cgttttttcg
     5821 atatcaagcc atgcccgtgc tgacagaagt aatctactgc accgcaggct tttaacacct
     5881 gcaacggttg atggcagtcc gggcaaagag ctttcatttc aataaattcc ccgcaggatg
     5941 ggcaacgcgc atggccgtta tcctgatcaa gcacatgctg acattgtgga caatgcagtt
     6001 ccatatgaat tcctccggta gccattccct ctacattcat agagggaatg gcagataaaa
     6061 tacttacgga taattattta tttttcttga tgtgcttcat cagacgctta cgtttacgca
     6121 tctgggttgg cgtcagggtg ttacgcttat tcgcatacgg gttttcccct tctttgaact
     6181 gaatacgaat cggcgatccc attacgtcca gcgatttgcg gaagtagttc atcaagtaac
     6241 gcttgtagga atcaggcagg tctttcacct gattaccgtg aatcaccaca atcggcgggt
     6301 tataaccacc ggcgtgggca tatttcagct tcacacgacg accgcgtaca agcggcggtt
     6361 ggtgatcttc aacagccatc gtcatgatgc gcgtcagcat agaggtcccc acacgacggg
     6421 tggagctgtc atacgcttca cgtactgatt caaacaagtt accaacacca ctgccgtgca
     6481 aggcagagat aaagtgcaca cgagcaaaat cgataaagcc cagacggaag tccagcgttt
     6541 ctttcacctg ctctttcact tcctgactca ggccatccca cttattcacc acaatgacaa
     6601 gtgagcgccc actattgaga ataaagccca gcagcgagag atcctgatcg gaaatacctt
     6661 cgcgcgcatc aatcactaac atcaccacgt tggcatcttc aatggcctgc aacgttttga
     6721 ttacggagaa tttctctaca gcatcggtga ttttaccgcg tttacgtacg ccagcggtgt
     6781 caatgagcac atactcacgt ccatcgcgtt ccattggaat gtagatactg tcacgcgtcg
     6841 taccaggcat gtcataaaca acaacgcgct cttcaccaag aatacggtta gtgagtgtag
     6901 acttacctac gttcggacga cccacaatcg ccagtttgat cggcagactt tgcgggtcga
     6961 agtcgtcttc ctcttcttct tcgccgttct cttccgcttc aaattgcgcc cagtattcag
     7021 cgtcttcgtc gacttcctct tgcggtgcga gatcttccat ccacggcagt agcacatgct
     7081 ccagcagact taatacgcca cgcccatgag aagcggcaat tgggtagatt tcgcctaaac
     7141 cgagcgaata gaaatcaacg actgcctggt ctggatctaa accatcagtt ttgtttgcca
     7201 ccaggaaggt aggtttttct cgggagcgta ggtgcttagc aatcgcttca tctgccggca
     7261 tcaggcccgc gcgcgcatcc accataaaca gtacgacgtc cgcttcttca atcgccagca
     7321 gcgactgttc cgccatgcgg gtttctacac cgtcttctgt gccatcaatc ccgccggtat
     7381 caatacagat aaactcacgg ccttcaattt ccgcacgacc gtacttacgg tcacgagtca
     7441 gacccgggaa atccgcaacc agcgcatctc gggtgcgagt tagacggtta aataacgtgg
     7501 attttcctac gttagggcgc ccgacaagcg cgaccacagg taccatgttt aaagcctcat
     7561 ttttataaat catcagacaa cgcacgctat attcgcgtcg ttgttaaaaa caggaaaacg
     7621 gcccctgtcc aggagccgtt ttcaaagtga acgacagaga cgattaacgt gtaatagagt
     7681 acacggttcc gtcttttgcc tggatcagca gtttgccgtc agcggcaacc ggttcagtct
     7741 ggaaaccgga actatcaact ttttgctggg caacgaaacg accatcttcg acgttaatcc
     7801 agtgcaaata accttcactg tcaccgacca ccaggttgcc attatacagc accggagaag
     7861 tcagcaggcg atgcagcaga tcgctttgtg tccacagcgt aacgccgcca tcaatggtca
     7921 acgccatcac ccggtcattt tgatcgacca gatagatgcg attgccgtcg acgatgaaat
     7981 cattcaccga acccagttcg cgtttccaca taatctgacc actgcgcaga tcaagcgccg
     8041 tcaggttacc attataggcc agcgcgaaaa caacgccgtt aacaacgacg ggagtcgtgt
     8101 caacatcgct cagacggtca atttcggtag aaccggtcgc ctgggaaata cgctgctgcc
     8161 aaatcatctg gccctgttcc atcagcactg cgctgacgcg accattatcg ccccccacga
     8221 cggctgcacc aaaagccgtt gccggcgcag actcgccacg caaagagagc gaaggcatat
     8281 cgaggttaac tgtccatttg acagcgccgt cagcttcgtt cagcgcttgt aactgaccgt
     8341 tactggtgtg gattaacacc agaccgtcgc tgaccaccgg gcgcgaaagt gcttcacccg
     8401 cgactttagt ttgccatgcc acagtaccat cgctggtatt cagcgcgtaa acctgcgcct
     8461 tttcgctgcc aatgtagaca tgtccaccag acacggtcac accgccagaa agtaatgcag
     8521 gctctttaga gaaccagcca tctttctcgg ccaggctgac agaccagatt tctttgccat
     8581 catccgcatt cagcgctttt actaaaccag cgcggtccgc tgcatagaca acgttgtccg
     8641 ccagtgccgg atgaagattg gaatagaagt tgccaatgcc gctaccaacg gaagtgctcc
     8701 acgccgtggt cggcgtaaac tggttttcaa cggttggcaa tggggacatc tttaccacat
     8761 cttcttcgct gttaaacagc gaacagccgc ttaaaagggt aacggaaagc agtcctggca
     8821 gcagtaattt acgcaattgc atcgggtccc tctcagatgg acaaattatt aattttcatc
     8881 tgcatcattt cgctcagtgc cggagtaaca tcgcttttca cgcctgcttc ccatgcacta
     8941 cgcgcacctt gcttatcacc tttgctcagc aatgcttcac cacgcaggtc ggcaacaatg
     9001 gcagcccacc cttcaccttt gatggtatca agggttttca gcgcggcatc agcctgcttg
     9061 agctgtacct gaacgcgagc aagacgcaga tttatcacgg ctttgagatt ttcatcgctc
     9121 gtgtctgcca gcccctgttg taactgggcg gcagctttct ctagttcatt tttgtcaaca
     9181 aattgctgcg caagttccaa agaagccagc gcaccataag tatttttatt ttcagcagca
     9241 aatttttccg ccgccgggat gctatccggt ttgccttcgc tcactgcagt aaccgcattt
     9301 tgataggcaa gagaagcgga gcgtgcagaa tcaacctgat ggctgttcca gtagcgccag
     9361 ccaatcagtg cgccaacgcc caaaatcacc ccaacagcca gtgctttgcc attttcagca
     9421 aaaaagcgtt taaccgcttc tacctggtcg ttttcgttct cgtaaatttc cacgctgtcc
     9481 ttctccttcc ttaacccagt aacgtgcgca aatgcgcggc tacgctatcc tgcgcaactg
     9541 ccgtttgctc accagagcgc aaatccttca ctactgctgt gccgttagcc acttcagact
     9601 cacccagcac cacagcaacg cgggcacccc atttatcagc acgggcaaac tgtttcttaa
     9661 agttgccgcc gccgtggttg gtcatcaatt tcacgcccgg taattcatca cgcagacgct
     9721 cagctaatgc catagccgca gattgtgtat cagcacctga agccaccagg tatatatcga
     9781 caacaggatc ggctttaaat tccggattaa cggcctgtac taacaataca agacgttcga
     9841 ggcccatcgc aaaaccgaca gccggtgttg cacgaccgcc cagttgttcc acaagaccgt
     9901 cataacgacc gcctgcacac acggtgccct gggagccgag actgttagtc acccactcga
     9961 aaacggtacg gttgtagtaa tccagaccac gcaccagacg ctggtttacg gtgtaaacga
    10021 tccccgcgct ctccagcagt ttgcacagac cggcaaaatg ctcacgagat tcctcgtcca
    10081 gataatcgcc taatgccgga gcgtcgttga gaagcgcctg cacttctgga ttttttgaat
    10141 ccagcacgcg cagcgggtta gtgtacatgc ggcgtttgca gtcttcgtcc agcttttctt
    10201 tatgctgctc aaggaatgcc accagcgcat cgcggtaatt ggcgcgtgct tccagcgaac
    10261 cgatagagtt cagctcaaga gttacgtgct cggaaatacc cagcgcgcgc caccagcggg
    10321 cggtgagcat aatcagttca gcgtcgatat ccggaccttg cagaccgaaa acttcgcagc
    10381 ccaactgatg gaactgacga taacgccctt tctgcggacg ctcgtgacgg aacatcggcc
    10441 cgatatacca cagacgctgt tcctgattgt acagaagacc atgctcgatg ccggcgcgta
    10501 cacagcccgc cgtcccttca gggcgcagag tcaggctgtc gccattgcga tcctcaaagg
    10561 tgtacatctc tttttcaacc acgtcggtga cttcaccaat cgcacgtttg aatagcgggg
    10621 tctgctctac aatcggcaag cggatttcac tgtaaccgta gctgccgagc acgtttttca
    10681 gtgtgccttc aatgcgctgc cagatggccg tttcgccagg caggtaatcg ttcatgccgc
    10741 gaatggcttg aatgtttttt gccacgttta ttctctttct gaatataaaa atgaaccctc
    10801 aacgcttccc tcaatgtttc gggagccatg cgggttcaat catacacggg aagcgaggcg
    10861 cttcccatca cgttattatt tttcaacctg ctgaacgtca attcgacgcg cttcgtccag
    10921 ctgactggct ttcacacgaa tgcgcgcttc cagctggtcg atcatatcgt tgttgtccag
    10981 acggtctttg cgtacgccat cttcatagag gccgcttttc ttgttgccgc cggtgacgcc
    11041 gagtgtagaa accagcgcct cacctgggcc attcaccacg cagccgataa tcgaaacgtc
    11101 catcggagtg atgatatctt ccaggcgttg ctccagcgcg ttaaccgtac cgataacatc
    11161 aaactcctga cgcgaacagg tcggacaggc gatgaagttg atacctcgcg aacggatacg
    11221 cagcgatttc aaaatatcga aaccgacttt gatctcttcg accggatcgg ccgccagtga
    11281 tacgcgcagc gtgtcgccga tgccttcaga cagcagcaga cctaaaccaa tggcggattt
    11341 tactgccccg ctgcgcgcgc cacccgcttc ggtgatcccc agatgcagcg gctgatcgat
    11401 ttgttttgcc agcaaacgat aagactcaac agcgaggaag acgtcagacg ctttcacgct
    11461 gactttgaac tgatcgaagt tcaggcgatc gagatgatca acatggcgca tggcggattc
    11521 cagcaacgcc tgcggcgtcg gttcgccata cttttcttgc agatcttttt ccagcgatcc
    11581 ggcgttaacg ccaatacgga tcggaatgtt tttatcgcgc gcacagtcaa ccaccatgcg
    11641 aatacgctct tcattaccga tattgccagg gttaatacgc agacaatcga cgccgtattc
    11701 cgctactttc agcgcaatgc gatagtcgaa gtggatgtca gccaccagcg gcacgttaac
    11761 ctgctgtttg atgagtttga acgcttctgc cgcgtccatc gtcggtacgg agacacggac
    11821 gatatcagcg ccaacgcgtt ccagcgcctt gatttgattg accgttgctt cgacatctgt
    11881 cgtacgcgta ttggtcatgg actgtacggc gatgggagca ccatcgccaa tcggcacatt
    11941 cccaacgtaa atacgtgttg attttctacg ttgaattgga gcctggttat gcatgaaaaa
    12001 tctcccgcgt tacccgtctg ttactgcgcc ggtgattgtt cggcattgag ggtcagacgc
    12061 gcaacctggt tagttctgat aaaacgactc agatcgacag gtttcccttg atactggatc
    12121 tgtactgcgg ctggcgcacc aattttcagt ttgtatggtg cctggccggt taagttcaaa
    12181 ttaccgtctt tacgctgcat accgctaaac aattttttac cggtagcatc agtgacctcc
    12241 agccagcaat cggcagtaaa gttcatcacc agcgcattcg gatcagccgc cggcgtagtc
    12301 acgcctgcct gatcggttgg caacggcgca gcgccatctg gcgttgttgt tgccgttggt
    12361 gccggggtcg cggcggtatc aacatttgcc tgcgaaggcg aaacaaccgc attctgttgc
    12421 ggatcaacag ctggtgctgg cgcagttacg gcaggtgttt gtgtgttggt tgcggtagta
    12481 tccacagacg ctggcggcgt ggacgttgta gccgggtctg tagttgtcga cgtatttaac
    12541 ggaacactct gcccctgctc gctattactg ctcagttccg ccgaagattg atcggccatc
    12601 gtggtgatct cttcctgctg agctttgtgg tcttgccacc accaggcacc gctcaggccg
    12661 ataaccacaa acaacaccag ccaggtgaag gtcatcagcc agccgtcacg ttttttgcgg
    12721 cgtttaccga gggaaaaact ctgcatcggc gcaacttttg cagcccgtag tggagcctgc
    12781 ttttccagcc ctggcagcag ttcttcttct ggaatatgta ccagacgcgc ataagagcgg
    12841 atatatccgc gcaggaatgt tgaagcaaga tcggcgggtg ccttatcttc ttcaatgtcg
    12901 cgtaccgtgg aaaccttcag gcaaagtcgc tcggcaacgg cctgctgact aagtcctagt
    12961 tgttcgcgag cattacgcag gcgagcgccg gtagtaagtg cttcattttg gtcgtgcgtg
    13021 gcttcagtat tcattcgctg caggtacgtt taaatgagaa ttgagatgcc ggtgaaccat
    13081 catgctcacc cacaccgcga aacatccgtt aagttaaccg ttatcataca gtataagacc
    13141 gtctgtgtgc tcgtgacaaa gcccacattc actaaggcta acttactgtt gcatcgttac
    13201 atactgcctt aaagtcagca aaaacgcacc gttaatattg accagacaat tgcaacttaa
    13261 tacttcattc ataaaacatt acgccacggt acataaagta accgtggcgt aatggctatc
    13321 agaccgcttt aatgtcgatg gcttcaccct gcatccgttt acgcagggta cgtttcgtac
    13381 ggtcgataac atcgcccgcc aactgaccac aggcagcatc gatatcatca ccacgagttt
    13441 tacgcacaat agtggtgaaa ccgtagctca tcagcacttt tgagaaacgg tcgatacggc
    13501 tgttcgagct gcgtccatac ggcgcacccg ggaacgggtt ccacgggatc aggttgatct
    13561 tacacggcgt atctttcagc agttccgcca gttggtgcgc gtgttcagtg ccgtcgttaa
    13621 cgtggtcaag catcacgtat tcaatagtga ctcggccctg attggcgttg gatttctcca
    13681 gataacggcg caccgcagca aggaacgttt cgatattgta ctttttgttg atcggcacaa
    13741 tttcgtcacg aatttcgtcg ttcggcgcgt gcagggaaat tgccagtgca acgtcgatca
    13801 tatcgcccag tttatccagc gccggaacta caccggaagt ggaaagcgtg acgcgacgtt
    13861 tagacaggcc aaaaccgaaa tcatcaagca tgatttccat cgccggaacg acgttgttca
    13921 ggttgagcag cggctcgccc atgcccatca tcactacgtt agtgattggg cgctgaccgg
    13981 tgacttttgc tgcgccgacg attttcgccg cacgccacac ctggccgata atttccgaca
    14041 cccgcaggtt gcggttaaag ccctgctggg cggtggaaca gaatttacac tccagcgcac
    14101 accccacctg cgaagagacg cagagcgtgg cacggtcgtc ttccgggatg tacaccgttt
    14161 cgacgcgctg atcgccaacg gcgatcgccc atttaatggt gccgtcggat gaacgctgtt
    14221 cttcaaccac ttccggtgcg cggatttccg ccacctcttt cagtttgccg cgcaacactt
    14281 tgttgatgtc ggtcatctca tcaaagttgt cgcagcaata gtgatacatc cacttcatca
    14341 cctgatcggc gcggaagggt ttttcaccta aatctttaaa aaactcccgc atctgctgac
    14401 ggttgagatc cagcaggttg atttttccat ctttcgtggt gacgttttca ggtgtgacta
    14461 attgttcaga catatgctat tccggcctcg ttattacacg ttatggcccc tggagggttg
    14521 aaaaaagaaa cgccccggtg agcggctgct cgtccggggg cgctgcattg tacaaattct
    14581 ggcgcacgga tgccacgttt gcacgcggca tttacgaaat tattaacggg tgcgcgggca
    14641 cacttcgcct ttgccaaaga aataagcgat ttcgcgagcg gcagattcga cggaatcaga
    14701 accgtgggta ccgttttcgg tcaggctgtc agcgtaatca gcgcgcagag taccagccag
    14761 tgcgtttgcc ggattggtcg cgcccagcag atcgcggtga cgctgaacgg cgttttcacc
    14821 ttccagcacg gaaaccacga tcgggccaga ggtcatgaat tcaaccagac catcaaagaa
    14881 cggttttcca tcgtgttcag cataaaagcc acgtgcctgt tcaacggtca ggtgcagcat
    14941 tttggtgccg acaattttga accctgcagc ttcaaagcgc gcaaagatat taccaatgac
    15001 gttttttgct accgcgttcg gtttgatgat ggaaaaagta cgttcaatag ccatttttac
    15061 ctctgtaaat tgttctgttg ttgtctgtac cagcgtacga gatggcgcgg attataatga
    15121 gcaacagggc cgttgactat tgatgaaggt aacatttttt taaaataaaa tgagttttag
    15181 caacaatcag gggcaaaaca gactattgca tgacaaattt cactgtcgcg atttgcccca
    15241 catcatccat caccagcaat tgataatcac ctttatccgt caaatgcagg gtcacgttgc
    15301 gcccgcgttc agttaacggt tcgccattca gaaaccacca gcgttcacct gcccctccac
    15361 ttgattgcaa cggcaaagtc gcttccgctg cgcccggtaa acgtttaata atcgcgccat
    15421 cgcgaacgcc tgtcagctgc agagggagct tggcatcgtg accgtatggc gggcaaattg
    15481 tcgaggccgg tggtaagcgc acagcgcgac gttctgatgc gggcagccag ggttccagcg
    15541 gtagcggcca gacatttatc atttcttgtc gcgcttgcgg gcaatcagcg gcaacacgtt
    15601 tgccattttc atccagccag atggggaaac gaatgccatt aatgccttcc tgctccggca
    15661 gtaatagagt tggcggctga ctcccgtcca gcagccaggt tgccaggcgg cggcgacagt
    15721 taccgtcacc ttccggcaaa gactgtccgc ccggccagca gataacgcca cgagtgactg
    15781 agttcgggcg cgggtcttcc ggcagattcg cactgcgcga cagtaagata ttattgacct
    15841 gattcaacaa tggtacggca ctggcaaagc caaactgacc aacaacgggc gtgccgtccg
    15901 gtctgccagt ccagatccca atgacatagc gagcgttaac cccaatcgcc caggcgtcac
    15961 gatagccata gctggtgccc gttttccatg ccagtggggc gacgcgcggc aaggcactat
    16021 ccggcaaggg tttcgcttca tcagccataa tccggcgaat gatccacgcc gcccccgacg
    16081 acattaaagg ccgttcaagc agcggatcgt caggctgtaa gcgcaatttg cctgccttgc
    16141 cgtggcgagc aaacgcggta tacgctgccg ccatatcttc cagttttgca ccagcgccgc
    16201 cgagaatgag tgaaagattc ggcgcagcac cgttgggcaa atataacggc aatccaacat
    16261 tgcgtaactt tgccgcaaac cgtttcggtc cataggcttc caacacctgc acagcaggta
    16321 agttcagcga gcgcaccagc gcctcgctca tgctgatcgg gccatgaaaa ccgctatcaa
    16381 agttacctgg tcgataatca ccggtgcgcc gggggacgtc ttgcagcagt gatgccgggt
    16441 ggatcaagcc ttcatccagc gccagaccat aaacaaacgg tttgagcacc gatcctggcg
    16501 atcggatcgc attgaccata tcaacatgac caaagcgtga atcatcgttg agatcaaccg
    16561 atcccaccca gccgcgaaca cgcatatcgg tatgatcaac cacgatcatc gccagtgagc
    16621 tgcgcggtgg caaccgccct ttccagtttt gcgccagttc ttccagacgt cgttgaagac
    16681 cggcatccag cgtagtaacg attttgtcgc ttttgctttt accgagcatc atgcgcgaaa
    16741 acagcggtgc cagttgcggc atttgtcggg gtgccagcca gatgggttct tcccttgact
    16801 cttttacctg ctcacgggac cacacacctt gcacggccat ccgttcgagc actttattac
    16861 gcgcggcttc ggcacgctcc ggccaacgat ccgggcgaag acggctgggc gcttgaggca
    16921 aaaccgccag cattgccgcc tcggaatagc ttaaattcgc aggcgacttt ccgagataag
    16981 cccaacttgc cgcaccgatc ccctgcaacg taccgccaaa cggagcgcgg ttaagataca
    17041 aggtcagaat ttcacgctta gacagatgcc attccagttg caacgcgcgc cagagctggc
    17101 gaattttgcc gccaaatgtt ttggggtgag gatcaagcag acgagcaacc tgcatagtga
    17161 gcgtgctgcc accggaaata acccgtcccg aagtgagatc ttgccatgct gcgcgcgcca
    17221 ccgagaatgg attcaccccc ggatgcttcc agaaccagcg atcttcataa ttgatcagcg
    17281 cttcaaggta acgtggagaa acatcttcga ttgttaccgg ataacgccag atgccgtcag
    17341 catcggcgaa gcgccatagc ggcgtaccat cctgcgccac gaccactcgt gcgggattga
    17401 cttcatgcag cggtagaggc cagagtttat ctgctcccca cgctgcaaga aaaaggagaa
    17461 atggcgcagc tgccagcgtt atccagcagc cgcgtttggt taacaagcga ggcatttacg
    17521 gtctgacaat cagcaaatct tcagccgcgc cggtcgcccg ccattgggga acatacattg
    17581 attccaccat cggttgcggt acctgatacg ttcccggcgt caccgcccgc gccagataca
    17641 ccagcgttac cggttggtat tcatcaacgg caaccgccgc cacaaagcga tcgtcacgga
    17701 actcaatgtg cttaatgctc gcctgctgca tctggttcag taagttttgc acttcgccac
    17761 cactttgctc caggctggcg ctaccgttcg ccagattctg attttccagt tccagacccg
    17821 caggcagcag atccacgact aacgcatccg gcacgctgtt actggctttt acctgcaacc
    17881 agaccagcac cagatcgccg ctacgtaacg agtccagcga tttgctctta ccatcagtac
    17941 caagaatatg acgctcgatt tgcagcacat tgttcgccgg taaaggtgcg gattgcggat
    18001 aaccgctggc atccatacgc aaccataacg gctgatcgcc actgtttctc acctgcaagg
    18061 tggcaagttg atcgctattc agattgctgt ttagcgtttt ctcgcctgtc agcggctcag
    18121 ctgagaaaga ggtttgcgcc tgccatttac ccggtaaatc ctgaatcgta cgggcagcca
    18181 ggaacaacgc gttactttcc tgcgtcgata gccagcgttc accaaacgcc tgctgcgaaa
    18241 gtgtgttcag caaagtgtac tgctcatcgg gtagcagttt attttcttcc agcaaggaga
    18301 gcattaacgc gttgtcgcgc agtggactac cgtaatcacc cagccatatc cgctcatcac
    18361 tatttctcgg cgttttcagc gccagcacaa tcgcttcttc accacgcgtt gcatcaccca
    18421 tggttttcag cgcaacgcca agttgcagca gcggtaaacc agaagcggca tctgcacgat
    18481 gctcccagat ttcacgcagc gcacccagcg gagccttttg ctgacgggcc aacaccagcg
    18541 cagcgtaaga ctgtacggcg aatttactgg ctttgagatt atccgcgtac gggatcgaca
    18601 tcatgcccgg atcttgtaaa tagcgcagca gacgctcatt accccggtta atggcgtctg
    18661 tcggcacgct gtaaccctgt tcgcctgcgc ggaccaggaa atccatcacg taagacgtca
    18721 gccagtactc ttcgtcaccg tttttatccc acagcgcaaa gccgccgtta tcacgttgca
    18781 tttgcagcaa acgggaaatg ccgatatcga ccgatgcacg gcgtttctca tcactgtcgc
    18841 ctttgatgcc caacgcctgc agttgggctg cgttggtata aagtgacggg aacaggccgc
    18901 tggcggtttg ctcaagacag ccgtacggat acgcttttaa ctctttgata taacgtgcga
    18961 tgttcagcgg tggtttgccg ctcaacaaca attgcccttc cagcgtaaca ggcgagaagt
    19021 tttgcaatcc atccgccgga atcgcccatg tctcaccagg ctgtaacgcc gtaccgtaat
    19081 taaccgtttg tgccgggaac gccggacgga cgccgatttt ccactgctta tgctgatcgg
    19141 caacggtttc acccggtaac gctaacccgc taatggtggc ctgaatttct ccatcgccat
    19201 aacccggcaa tgctcgcacc gggataaaca gcgtagtact cacacctggc gctaattcaa
    19261 cggctgcggg tgaatcgctg accagttcaa gcaaaccact ggcggtcagg gcaacgttca
    19321 gtttttgcgg tttatcggta agattagtga tatccagcgt cagacgcgag gtatcgccac
    19381 tcgccataaa gcgcggcatg ttcagttcag caatcaccgg tgcggcaact atcactttgc
    19441 tttcgttgct accgaagtca tctgccgtcc aggcttgcgc catgacgcgc aattcaccgt
    19501 taaaatcgcc aatcggcagt gtaaccgagc cttcgccctg ttcgttgagc gttaccggca
    19561 gcgcctgctg cgcgacaata ttgacgtgat tgaccggcgg tttaccacca cgtttcagct
    19621 catcaccatc gccaccgaaa cgcagagcag ccagacgccc ctgaccttca ataacctgac
    19681 cgtaaatatc gtaaatgtct gcgccatagc gtttctgacc aaagaacgcc tgccacggat
    19741 ctggcgtgac gtagtcagta atattcagca caccactatc aacggcagac accagcacat
    19801 tcacctgttt aggcttctcg ccatttttag tgctggcttt aattttcacg gttaatggtt
    19861 gattgggacg catttttgct ggtgtttcca gcgccagatc gaggcgacgg ttttcatcac
    19921 caagcggcag atgcaacaca ccaaccgcgc gttttggcgt cgcggagcga gatttatcgc
    19981 caggacgcac caccagcgta ctcaaataaa gatcatgacg attccaggtt ttatcgaccg
    20041 gaatcgtcag atccagccct tgagccggaa catcaatctc ttgccaccac agcggccctt
    20101 cactggactc gaccatcgca taacctttac ccgccgttgg cacggcaata tgcaacttaa
    20161 tggtatcgcc agggcgataa ctggctttat ctagtttcag cgtgacacgg tcgggtcgca
    20221 cggcaccgct cccgtcgctg ttgtcctgcc agctatagcc agcccagaaa cgaacactac
    20281 tgaccgcttc attcggcgct ttgacttcca gacgataagc gccccactct accggaaaac
    20341 tgaccttgcc gatttcgtcc gctttcagat ccagagtttg ttcattttcg atcagatctt
    20401 tttgatcaaa ctgtgattgc cagccttcat cttctgacca gttccagtag taatcgcggc
    20461 gttcgcgaat caggcgcacc tgcaagcccg acacggcttt tttcacgcct tgcgcatcgc
    20521 tataaacgat gtcaaaagcg gcgttactgc cttcatcaac aatgggctgt ttcaccgtgc
    20581 tgtcagtgcg ataatcgtaa accgatttcg aggcgaactg cggacggagc cccggcaatg
    20641 catcggcagg ccagatagcc tgctcagcac ggcgcgtcac cgggcgaccg cccgattcca
    20701 gcaaactgcc ctggaaaata acctgcaatg gggaatgcgt ttccttccac tggctttctg
    20761 tagaaacttc gccgcgccct ttatcatcca gcgtcaactg aacttcatcc agcgtgcggg
    20821 aaaggttttc ggcagctata tcgccgaatt caaaaccagg taaggctgac acagcctcac
    20881 gcagtgggcg caggaaaagt tgcccttgca aagtattacc attagcaggc gcaccgtaca
    20941 ggtagtatcc caccacggag aatttcactt catcattcgg cgtaagcggg gttttctcac
    21001 cggtcagatt cagcgccata cgctctggca taaaatcttc gacgtggaaa tcccacatcc
    21061 gatactggtt atcgcccgtg ttagcacgaa tatgccacat accggttgcc gcattgctat
    21121 cgagtggcca ggtaaagtgg tagaggccat tttccggctg actaacgacg ctcctgagta
    21181 cctgcccatc gggtttaatc acgtctaact tgatgggttg attgggcaac gctttaccgt
    21241 ctgcatcacg cagcaaacca ttgaggatga ccgtttcacc cgggcgataa agatcgcgcg
    21301 ggccaaacat gaaaaactgt ttgctatagc ctggcgcgcc agcaatgtta aattctgcta
    21361 agtccagcgc cggaagtttt aaatcgagta gcgttgtctg accgtcttta cgtgccagca
    21421 gtaatgcggc gtttttatca ttttccagct gcacatgccc ctgagcgtca ctggttgcct
    21481 gagtcagagt ctgccctttc tcatttaata aagagacttc aattccttgc tgggccgcgc
    21541 cgttttccag actttgggta aagatatcca gacgattgtg ataacggtga gctgaaacgc
    21601 cgatatcact taacgtaaac agcgtcgcgg gattactgta atcgtaacgt ccagcctgat
    21661 tcatcacagc cagatacacg cccgcctgct gaagcggttt gatatcgccc agcggcagca
    21721 ataatttttc acgggtgtta cgcgcaggat tgagatcaaa ccgtccggtg tagaccagat
    21781 ccgccatctg cagcagtttg tctgactgcc agttcgccag cgaattacgg tattcccatt
    21841 ggctaatgaa tgctggcaga gattctggct taacgcggaa gaagttaaca tcgacattat
    21901 taacgttgag cgccattacc ggcagccctt caacgacttt accaggcagc agcgaaccac
    21961 ggctggcaaa accgacgctg ggttggatgt cgcgggtagt tatagttttt tcgtaatctt
    22021 tactgaaggt tgcgttgttg agtgctttga cctccttgcc aatagtaacg atcaaatcac
    22081 gtttcggttc gaggtggcgt aaacgcagct ctttaagatt atctgacagc tcccaggcac
    22141 catccacttt gccgcttttt ttatcgacga catgaataac gcgtgagaaa tcctgatccg
    22201 ggtcgagagg gatggagaac gtcagcacca gcgtggctgc accatcaagt tggacttcag
    22261 agagatccag caatgtcagc accttaccgg cactctgttg ggccagtttt tgtcgctccg
    22321 gcacggagag ttttgctgaa ctcgcgtttt ctgaagaggc cgctttagta acttcagaag
    22381 gcgcatcttt tttcaccgct gttggcgcgt tatcgttgtt gtcgcaccct gccagcgcca
    22441 gcattagcat gcaggcggct acgcgtaact ttttcatttt tcatccctgc cacaatggcc
    22501 cgttagcaac gtcgaataat tattatgcgt gagaatttac gttatgtaat tcagtatcac
    22561 gcccatacaa tccgaatttt agtgagaatt gtggctcaga cagccaaatc agcctctttt
    22621 gcttgtcgct tttcactaaa catccgacaa tttagcctaa ccccggcaaa aatggagatg
    22681 cctatgtcca cgacatggtt tgtaggagcc gactggctcg ccgaacatat tgatgacccg
    22741 gaaattcaga ttatcgatgc ccgcatggcg tcgcctggac aggaggatcg taacgttgct
    22801 caggagtatc tgaatggaca tattcccggc gcagtgtttt ttgatatcga agcgctttct
    22861 gatcacactt ccccgcttcc gcacatgctg ccgcgcccgg aaacgttcgc cgtggcgatg
    22921 cgtgaattag gcgttaacca ggataagcac ctgattgtct atgacgaagg taatcttttc
    22981 tcagccccac gagcatggtg gatgctgcgc acctttggtg tagagaaagt gtcgattctg
    23041 gggggtggac ttgcaggctg gcagcgcgat gatctgttgt tagaagaagg tgcagtagag
    23101 ctgccggaag gagagtttaa cgccgcgttt aatcctgaag cagtggtgaa agtaaccgat
    23161 gtgttattgg caagccatga aaatacggcg caaattattg atgcccgccc ggctgcacgt
    23221 tttaacgcag aagttgatga acctcgccca ggtttacgtc gcggacatat tcccggtgca
    23281 ttgaacgttc cgtggacgga actggtgcgc gaaggcgaac taaaaacgac cgatgaactg
    23341 gatgcgatat tttttggtcg cggcgtcagc tatgacaaac cgattatcgt cagctgcggc
    23401 tctggtgtaa cagcagcagt ggttttgttg gcactcgcga cgctggatgt gccaaacgtg
    23461 aaactgtacg acggcgcatg gagtgaatgg ggcgcgcggg cagatttacc ggttgagcca
    23521 ttgaaataag ctttttacag gcaataaaaa accgccgaat ttggcggttt tttattgctg
    23581 gtctggttcg cggcctttcc agcaggttga cttgtgttac atgagcaacg caggtgcttc
    23641 acagcaaaac aatactcacc agtaactctc tttttgtcaa gcaaaagaga gtaattattg
    23701 tttatttagc gtattatcga caccggccct ttccgccgtg ttcggtaata aaataacctg
    23761 gcttattagt ctgaattcag acaaatataa acaaatcctg ctcaaaatta aaaattctaa
    23821 ccggtaaaag atattactta aacatgtaaa ttcactttcc tttaaaaaac aaaaaaccgc
    23881 caaaatcagg cggttatttg ttgctggtcc ggttcgcggc ctttccagca ggttgtatta
    23941 ccttagtaat gcaagcgcgt ctcagcggag acaatactcg ccagtaactc tctttttgtc
    24001 aagcaaaaga gagttattat tgttctgtta gtgtattatc cactgcggcc ctttccgccg
    24061 tctcgcaagc gggcgctggc ttaaggaaag gatgttccgc gaccgttaga gcaagcgcgt
    24121 ctcagcgctt gctatcgcgg caatatcgcc agtggtgctg tcgtgatgcg gtcttcgcat
    24181 ggaccgcaca atgaagatac ggtgcttctg tatcgtactt attgtttctg gtgcgctgtt
    24241 aaccgaggta aataataacc ggagtctctc cggcgacaat ttactggtgg ttaacaacct
    24301 tcagagcagc aggtaagccc gaatgccgcc ctttagggcg gcatatttta gattatccgg
    24361 ttttgtttaa agtcacgcaa aaaaccaccc cagcgacgtt catagaatgg cacaatatgt
    24421 tcggtaataa agtggctaat tcctttttcc ccttttttca cctgacaaat atcgattggt
    24481 tcatcgccag gtaatgtatc ggtcgctaca cttcccgtcg cctgaataat ttcttcgata
    24541 tcaccatcgg cttcaatgcc aataagtaaa ttaggctgtg cctcttcgtt ctctttaatt
    24601 gaacaaataa aagcacgctt caccggctta atggttttaa ataaggtggt gagtgaatca
    24661 atcatttgtg ctggcggctc tgcgacttcc gataatatca gcgattcacc gccttccagg
    24721 atttcctggc tgctcagcgg atttccctct tcaccaatca acaaactgat ttcacgcggc
    24781 ataaattctt taccggtcgg cagtttggca ttaaggaaga gcgtttcgcc aagggtcatc
    24841 tcaaacaatg tgcgaacggg cattacgaca aatgcctgtt cgtcttcaac cgcctgttga
    24901 agcgcttcta acgaggtgaa aaaaggaatg acgctggtgc cgtcttcttt ttcccagtgc
    24961 tgtaaatcaa gcgcactatc ttcaaccaca gcctcgccct gcgccgccgt accaggcacc
    25021 cagacagtgg attccagtag agtacggaaa aaggccgggc ggtgcgccgg ttcggttgct
    25081 gctttttcca gcaggtcttc aagttcgttt tttgtttcgg acataagaac cacaattcat
    25141 tcaacgttcg gggcgcaaat gcctgatgcg ctacgcttat caggcctaca agggattcgc
    25201 aatttgttga atttgcagaa tttgtaggcc ggataaggcg tttacgccgc atccggcata
    25261 ttagtttacg ccgttaacag attagctatc gtgcgcacac caagtcccgt agcgcccgca
    25321 gaccactgtt caaccggcgc tttacggtaa gtcgccgagc agtcgatatg cagccagcct
    25381 tgctgatagt tctcaacaaa gtgcgacagg aagcccgccg ccgtgctcgc gcctgccgga
    25441 tacgccgcgc ttccggtatt gttcagttcg gcaaagttag acggcagctg gctgcggtgg
    25501 aactccgcca gcggcagacg ccagaatggt tcgttctctt gtgaggcact cgccagcaaa
    25561 cgaccggcaa gcgcatcgtc aaaactgaac agcgcgtgat aatcattacc cagcgcagtt
    25621 ttcgccgccc cggtgagggt cgccgcatca atgatcattt ccggtttctg cgcactggca
    25681 tcaatcagac catcggcaag caccagacgc ccttcggcat cagtgttcat cacttcaact
    25741 tttttaccgt tgcgataggt gatgatatcg cccagcttga acgcattgcc gctaatcagg
    25801 ttatccgcac agcagaggaa cagcttcacg cgcttgttca gtccgcgcgt aatggcaaat
    25861 gccagcgccc cggtaaccgt tgccgcgccg cccatgtccg acttcatcga gtccataaac
    25921 gcagtctgtt tgatgctgta gccgccggag tcaaaagtga tacctttacc taccaggcac
    25981 gcgtacactg gcgcttcttt atcgccagtt gggttgtaat ccagcgccag caataccgga
    26041 gaacgttctg aaccgcgtcc gacggtgtgc agccccatat aaccttgctc acgcagatct
    26101 tcgcctttgg tgatccgata agtcacacga tcgcccgcga cgttgctgat cagatcaaca
    26161 gcacgctgtg ccaattgcga tggtcccaat tcttctgccg gtgcgttgat ggtgtcacgc
    26221 acccagtcga tgatcatcag gcggttatcc agttcctggc gctgggcatc gtccagatcc
    26281 ggccactcca ctttacgcgt gcctttcggg gctttgtaac cttgccagaa tgcccagcag
    26341 cgatccgcat cccagccttc accgcttaac tgaacatgct tgatgcccag accgtcaatc
    26401 ttgcgcgccg cacgctggat cagccccaga tcgtctgccc cgttcaaatg cagggtaatg
    26461 ccgtcattat taatgctgta agttgctttt tctccccagc gcgcgtcggc aggttgggta
    26521 gagagggtaa tcttcatcgc ttctgtcatt ttagttatcc ttctttgtaa aagggccgcc
    26581 agttggcagc cccaggattt ttccgctaaa tgattcgcgt tgcaggaagg cggcaagtga
    26641 gtgaagcccc aggagcatag ataactatgt gactggggtg aacgagcgca gccaacgcat
    26701 ctgcggcgtg aagcatgacg cggaaattat tcggcctcgt ccagccagac taacaaaatc
    26761 gcttcgagga ttttctcgtt ggatgcctgc gggtcgtcgt cgaaatcttc cagatcgcaa
    26821 atccactgat gcatatcggt gaatcgaacc gttttcggat caagatcggg atacgcatcg
    26881 tacagtgcct cgccaatttc gcggctatcg gtccacttaa gtcccatact aacctctgtt
    26941 aatgctcacg cgcatggttg atagtgtaac gcgggatttc gactactaaa tcttcgtcgg
    27001 taacgcgcgc ctggcagctt aaacggcttt cgggctccag tccccaggct ttgtccagca
    27061 tgtcgtcttc ctgctctgag ctttccggca gcgagtcaaa accttcacga acgatgcagt
    27121 ggcaggtggt gcaagcacag gatttttcac aggcgtgttc aatctcgata ccgttacgca
    27181 gagctgcgtc gagaatggtt tcaccgctat tagcttccag aacagcgcca tcaggacaga
    27241 gatcctgatg aggcaaaata acaatctttg gcatattaaa cctcgtccac ggaatggcct
    27301 ttcagcgcac gacgaaccga ctggtccatg cggcgagcgg cgaaatcctg ggtttgtttg
    27361 tctacgtttt taatcgcttg ttcgatggcg tcaacgtcat cgccctgcgc cacttcactc
    27421 aggtgagcgg cagcatcgtc aatgacctga cgttctgcgg cgcttaacag cgcggcatca
    27481 gcagccagcg cgccgtgcag actttccagc acacgcgccg cttctacttt ttgttctgcc
    27541 agcattcggg cttttacgtc ctgctcggca tagctcattg agtctttgat catcgaagcg
    27601 atttcgctat cggtcagacc gtaagacggt ttgacctgaa tagacgcctc aacgccggtg
    27661 gatttctcca tcgccgtcac gctcaaaaga ccgtcggcat cgacctggaa cgtcacgcga
    27721 atatgcgcac cgccagccgg tagcgccgga ataccacgca gcgcaaaacg cgccagtgag
    27781 cggcagtcct gcaccagttc gcgctcaccc tgcattacat ggatagacat cgccgtctga
    27841 ccatctttaa aggtggtgaa atcctgagcg cgggccaccg gaatagtggt attacgcgga
    27901 atcactttct ccaccaggcc gcccatcgtt tcgaggccca gcgacagtgg gatcacatca
    27961 agcagcagca tttcgctgtc tggcttgtta cccaccagaa tatccgcctg aatcgccgcg
    28021 ccaatagcga cgactttatc cgggtcgatg gaagtcagcg gtggacgacc gaaaaattcg
    28081 cctacccgtt cacgcaccag cggcacgcga gtagaaccgc ccaccatcac cacttccagc
    28141 acttcatcag cttctacgcc cgcgtctttc agcgcgcgac gacaagccag taaggttcgt
    28201 tttaccagtg gcgcgatcag ttcattgaat tgttcacggc tgatttcgcc ctgccagccc
    28261 gcaacgttaa cggtcacgga gtccgcatcg ctcagggcga ttttggccgc aatggcggca
    28321 tccagcagtt cacgctgaac gcggttatcg ctacgatcag gaatgcccgc ctgttcgcga
    28381 atgtaatccg ccagtagatg gtcgaaatca tcgccgccga gcgcggaatc accgccggtt
    28441 gccagcactt caaacacgcc gcgacttaag cgcagaatgg aaatatcaaa cgtcccgcca
    28501 ccgaggtcat aaacagcgat cacgccttcc tgaccggaat ccagcccgta ggcgatagcc
    28561 gcagcggtcg gttcgttaag taagcgcagg acgtgaaggc ccgccagacg cgccgcgtct
    28621 ttggtgccct gacgctgggc atcgtcaaag tacgccggaa cggtgataac tacaccatcc
    28681 agctcgcctg ccagggcttc agttgcccgc gccgccagtg ctttgaggat gtccgcagaa
    28741 acgcgcaccg ggttcagcag ccccgccgcc gtttcaatca tcggcaggcc gttttcgctg
    28801 gcctggaatt gataaggcag atgcggatag cgttgctgga tatcagccag cgagcgcccc
    28861 atcaggcgtt taacagaact aatggtgttg gcggtgtcaa gcgctgcgtt agtacgcgcg
    28921 tcataaccca ccgaatgccc ttgctgttga tagtgaacaa cagatggcag caggtgacgg
    28981 ccttcatggt cggctaacgt ttcggcctga ccgctgcgca ctgtcgccac cagcgagttg
    29041 gttgtgccca ggtcaatacc agccgccaga cgacgctgat gcggcgcagc actcaaacca
    29101 ggttcactaa tttgtaataa ggccatgttt agcttccaga aattaaaaat cgagcagttt
    29161 ttcttcgagt tgttcggcac tgctccgcag tttatcgaga aaacgcagct tacgcacggt
    29221 atccgccgcc gcgtcccacg cctcgttgtc taactgttca accatcaact gatggcgggt
    29281 atcaaacatc tttttcacac gtttgataaa gctttccagc cgcgcttcat ctttcgcctg
    29341 ttcgatctcg tccagctctt cgcgcagctc caactgttcc atcaggaacg cggtgtcgcg
    29401 cacagtatgc tgctcgctgg cgagatcaaa accgtgtaaa gaaagcaaat attccgcgcg
    29461 cattaacgga tgacgcagcg tttgccaggc ctggttaatg gttgcagatt gctgtacggc
    29521 ggcgagttgt tccgcctggc ttccgctggc gaatttatca ggatgatact gacgttgtag
    29581 atcctgaaaa cgcaggctca gcgcctgggt atcgagttga taacgggcag gcaagccaaa
    29641 gagggtgaag taatccataa cattctcagg gctgcgatcc gccgcgataa cggcgaatca
    29701 ggtaaaacaa accccacgcg caggcgacca cggtggggtt atcggtatgc gcatcaaacg
    29761 tggaagcttt cgccgcaacc acactcatct ttaacgttcg ggttggtgaa tttaaaccct
    29821 tcattcaggc cttcttttac gaagtccagc tgcgtaccgt ccagaaattg caggcttttg
    29881 ccatcgacca cgactttcac gcctttgtct tcaaacacga tgtcttccgg cgtcggttcg
    29941 tcaacaaatt ccagtacata agccatacct gaacatccgg aggttctcac gcccagacgc
    30001 aggccaaacc ctttgccgcg gttagccaga aaggtattta ctcgcgctgc tgcactgtcg
    30061 ctcagtgtaa tcgacataac caaacctcaa ctcttatttt gcttcacgtt tgcttttata
    30121 gtccgcaatg gcggctttga tcgcgtcttc tgccagaata gaacagtgaa ttttcaccgg
    30181 cggcagttca agttcttcag caatatcggt gtttttgatc gcctgcgctt cgtcgagaga
    30241 cttccctttc acccattcgg tgaccaggga gctggaagcg atagcagaac cgcagccgta
    30301 agttttaaaa cgcgcgtctt caatgatacc ttcatcgttg actttaatct gcaacttcat
    30361 cacgtcgcca caggccggtg cccccaccat gccgctgccg acgttctcgt cgttgttgtc
    30421 aaaggaaccc acgttacgcg gattctcgta atggtcgata actttttcgc tgtaagccat
    30481 tataaattct cctgattccg ataccgatta atgatgagcc cattcgatgc tgttcagatc
    30541 cacgccctgc ttgtacattt cccacagcgg agaaaggtca cgcagacgac cgatggattt
    30601 acgaactaac tcgatggtgt agtcgatctc ttcttcagta gtaaaacgac ctaaagagaa
    30661 acggatagag ctatgtgcca gctcgtcgtt cagccccagc gcgcgcagca cgtaggacgg
    30721 ttcgaggctt gctgacgtac aggcggaacc tgaagaaact gcgaggtctt tcagcgccat
    30781 aatcagcgac tcaccttcaa cgtagttgaa gctgacgttg agaatgttcg gcgcaccgtg
    30841 ttccaggtca ccgttcaggt aaacttcttc gatatctttg atgccgttcc acagacggtt
    30901 acgcaggccg cgcagacgtt ccatctcggt cgccatctct tcttttgcga tgcgataggc
    30961 ctcgcccatt ccgacgatct ggtgaacagg cagagtgccg gaacgcatac cgcgctcgtg
    31021 accgccgccg tgcatttgcg cttcgatgcg tacgcgcggt ttacgacgta catacagcgc
    31081 accgatacct ttcgggccat agattttgtg accggagaaa gacatcaggt caactttcaa
    31141 ctggctcagg tcgataggca gtttacccac gctctgggtt gcatcaacgt gatagataat
    31201 gccacgagca cggcacattt cgccgatagc cgcgatatcc tgcaccacgc cgatttcgtt
    31261 atttacgtgc atgatggaaa caaggatggt gtcgtcacgc atcgctgctt caagttcttt
    31321 caggtcgata atgccgttac gctgcggtgc caggtaggtg acttcaaaac cttcgcgctc
    31381 cagctgacgg caggtatcca gtaccgcttt gtgttcggtt ttgctggtga tgatgtgctt
    31441 gccttttttc tgataaaagt tggctgcacc tttgatcgcc aggttgtcag attcggttgc
    31501 accagaggta aagacgattt cacgcggatc agcgccgacc agatcggcaa tctgattacg
    31561 ggcgatatct accgcttctt cagcctgcca gccgaaacgg tgagaacgag aggccgggtt
    31621 accaaaggtt ccgtccatcg tcatgaactg catcattttc tcggcaacac gcgggtccac
    31681 cggcgtggtt gcggagtagt cgagataaat cggtaatttc attgctctat aaactccgta
    31741 catcactcaa tgcaaggaat caggctaccg gctggatgta cgaccgtgtt tacggagtat
    31801 ttagcactcc ggcctgattc tgaattcttt ttattaagcg cgtaacttaa cgtcgatcgc
    31861 gtcttgtgtg cgggtgcgtg gcgcgtcgtg agtatgctga cgaccagaca catccagcac
    31921 ttcctggtta ttaaccagtt cgcctaaagt aatgttgttg agaaaaccgg tgagacggtc
    31981 gctcaaatca cgccacagcg cgtgggtcag gcatttatcg ccgccctggc agccgccttt
    32041 accctgacaa cgggtggcat ctacagattc gtcaacggcg ctaatcactt cgccaacggc
    32101 gatgctgctg gcatctttgc ctaacagata accaccgcct ggtccacgta cgctggaaac
    32161 cagaccattt ttacgcagac gggaaaacag ttgttccaga taagaaaggg aaattccctg
    32221 acgttcggaa atatcagcca acggtaccgg gcccgcttca gagttgagcg caacgtcaag
    32281 cattgcggtc acggcatagc gccctttaga tgtcagtctc atgtcttact tcacctcaaa
    32341 ctcgcccctg cccggggttt tttattgtaa aagtgggggt attgcatagc agggtcaagt
    32401 ctgacattcc cgagtaaatt ggtcaactat ttacttgact gatttagtcg ggtatttaac
    32461 cttcagtgcc atttttttat cgtggcgttt gtacctgttg tcggatgcgg tgtgaacgcc
    32521 atatccgccc tggggttctg tacactgtag gcctgataag acgcattacg cgtcgcatca
    32581 ggcaacggct gtcggatgcg gtgtgaacgc cttatccgac ctacggttct gttcactgta
    32641 ggcctgataa gacgcgttaa gcgtcgcatc aggcaaccac tgttattctg ccttgttacc
    32701 tttattctgc tgctctatag aagccagaat cccgcgcagg atattcaatt cctggctttc
    32761 cgggcgcgca cgggtaaaca gacggcgcaa tttattcatc acctgccccg gatggttttc
    32821 acggataaaa ccggttgcca gcagcgtttg ttccagatga ccataaaaac gctccagatc
    32881 atcgaccagc ggatacggcg tctcttcatg ctcgacctgc tcgccgtttt cctgagttgc
    32941 cagccaggcc atgcgcactt cataggcaat aacctgaacc gccatcgcca ggttcagcga
    33001 gctgtattcc gggttagccg caatcgcgac atgataatgg catttctgca actcttcatt
    33061 ggtcaagccg acgcgctcac gaccaaacac cagcgccacc ggggtatttg ccgcttcagc
    33121 gacgcttttc agaccgcatt cgcgcgggtc gagcatcggc cacggcagcg tgcgggagcg
    33181 tgcgctggtg cccaccacca ggctacaacc ggctaacgct tcgtcgagcg tatcgacgat
    33241 gtgagcatta ccaatcacat cgctggcccc tgctgccagg gcaatcgcct gggagtcggg
    33301 tttcaccagt ggattaacca gccacagatt ggttaatccc attgttttca tggcacgggc
    33361 aacagaaccc atattgccgg tgtgtgacgt ctccaccagc acaattcgaa tattttgcag
    33421 cattgtcttt cttcatctaa agattattca cgcatcttat cataaaacga agacagatgc
    33481 cgatctcgct gctatactct gcgccgtttt cccgttcttt aacatccagt gagagagacc
    33541 gatgcatccg atgctgaaca tcgccgtgcg cgcagcgcgc aaggcgggta atttaattgc
    33601 caaaaactat gaaaccccgg acgctgtaga agcgagccag aaaggcagta acgatttcgt
    33661 gaccaacgta gataaagctg ccgaagcggt gattatcgac acgattcgta aatcttaccc
    33721 acagcacacc atcatcaccg aagaaagcgg tgaacttgaa ggtactgatc aggatgttca
    33781 atgggttatc gatccactgg atggtactac caactttatc aaacgtctgc cgcacttcgc
    33841 ggtatctatc gccgttcgta tcaaaggccg caccgaagtt gctgtggtat acgatcctat
    33901 gcgtaacgaa ctgttcaccg ccactcgcgg tcagggcgca cagctgaacg gctaccgtct
    33961 gcgcggtagc accgctcgcg atctcgacgg taccattctg gcgaccggct tcccgttcaa
    34021 agcaaaacag tacgccacta cctacatcaa catcgtcggc aaactgttca acgaatgtgc
    34081 agacttccgt cgtaccggtt ctgcggcgct ggatctggct tacgtcgctg cgggtcgtgt
    34141 tgacggtttc tttgaaatcg gtctgcgtcc gtgggatttc gcggcaggcg agctgctggt
    34201 tcgtgaagcg ggcggcatcg tcagcgactt caccggtggt cataactata tgctgaccgg
    34261 taacatcgtt gctggtaacc cacgtgttgt gaaagccatg ctggcgaaca tgcgtgacga
    34321 gttaagcgac gctctgaagc gttaatgact caggcgggtg atgtcactca cccgccctcg
    34381 cctttcaggc gctattctga aatacttcct caccgcttta ctttctttcg tcactctccc
    34441 accatctttc cccgattaat ggatgataaa gaagtcgcaa ggaaaggtta tggcactgcc
    34501 agtgaacaaa cgcgttccca aaattctgtt tattctcttt gttgttgcct tctgcgttta
    34561 tttagtgccg cgcgttgcca tcaacttctt ctattatcct gacgataaaa tttacggtcc
    34621 cgacccctgg tcggcggaat ccgtcgaatt tacggctaag gacggtactc gtctgcaagg
    34681 ctggtttatt ccttcttcga cgggccctgc tgacaacgcc atcgcaacca tcattcatgc
    34741 tcacggcaat gccggaaata tgtccgccca ctggccgctg gtcagttggt tacccgagcg
    34801 taatttcaac gtttttatgt ttgattatcg cgggtttggt aaatcaaaag gcacgccgtc
    34861 ccaggccgga ttgctggacg atacgcaaag cgccatcaat gtggtgcgcc atcgcagtga
    34921 tgtaaaccca caacggctgg tgctgttcgg gcagagtatt ggcggggcga atattcttgc
    34981 tgttattggt cagggtgatc gtgaaggcat acgtgcggtg atcctcgact ccacatttgc
    35041 ctcttatgca accatcgcca accagatgat ccccggcagt ggctacttac ttgatgagag
    35101 ttacagcggc gaaaattata tcgccagcgt cagcccgatc ccgcttttac tcattcacgg
    35161 taaagctgat cacgttatcc catggcagca cagcgaaaag ttgtatagcc tggcaaaaga
    35221 gccaaaacgg ttgatcctaa tcccggatgg cgaacacatt gatgcttttt ccgatcgcca
    35281 cggcgatgtt tatcgcgaac agatggtgga ctttatcctt agcgcgttga atccgcagaa
    35341 ctaacccatg atcgctagca cgataatcat tcacaaaacc accttaagac atgctaatcc
    35401 actggtcaga acagtttaag atgagaaaaa ttctgtgacg cttgccaaca tttctgatga
    35461 ttagcattcc cttcgtcatt tccctgagca aactttagct attcttatca attatgctta
    35521 tgggagatct acagatgatg cctacgcttg ctccaccatc tgtcctttcg gctccccagc
    35581 gccgctgcca gatcttgctg acgctctttc agccggggtt gaccgccacc acggcaactt
    35641 tcagcgagct taatggtgtg gatgatgata ttgccagtct tgatatcagc gaaacaggac
    35701 aggagatcct gcgctatcat caactcacac tgacggctgg ttatgacggt agctaccggg
    35761 ttgaaggtac agtgcttaac caacgtttgt gtttatttca ctggctacga cgtggtttcc
    35821 gtctgtgtcc atcatttatt accagccatt tcacccccgc cctgaagagt gaactgaagc
    35881 ggcgcggaat tgcgcgtaac ttttacgacg ataccaatct acaagcgtta gtgaatctct
    35941 gctcccgacg gctgcaaaaa cgctttgaaa cgcgcgatat tcatttcctg tgtctgtatc
    36001 tgcaatattg tttgctgcaa caccacgctg gaattacgcc tcagtttaat ccgctccaac
    36061 gtcgctgggc ggaatcctgc cttgaatttc aggtagcgca ggaaattgga cgccactggc
    36121 agcgtcgggc gctccagcct gtaccacctg atgagccact gtttatggca ctactttttt
    36181 ccatgttgcg ggttcccgat ccactgcggg atgcgcatca gcgagacaga caattgcgtc
    36241 agtctatcaa acgtctggta aaccattttc gtgagctggg aaatgttcgt ttttatgatg
    36301 aacaggggtt atgtgatcag ctttataccc acctcgccca ggcgttaaat cgcagtttgt
    36361 ttgccatcgg tattgataat accctgccgg aagagttcgc cagactgtac ccacgcctgg
    36421 tgcgcaccac ccgcgcggcg ctggccggat ttgaaagtga atacggcgtc catctttctg
    36481 atgaggaaag tggtctggtc gcggtgattt tcggtgcctg gctaatgcag gaaaacgatc
    36541 tgcatgaaaa acagattatt ctactgaccg ggaatgatag cgagcgagaa gcgcagattg
    36601 agcagcagtt acgcgaacta acgttactgc cgctcaacat taagcatata tcggtaaagg
    36661 catttttgca gacaggcgct ccgcgtggcg cggcactgat tattgcgcct tataccatgc
    36721 cattaccgct cttttcacca ccgctgatct atacggacct gacgctgaca acacatcaac
    36781 aggagcagat ccgcaaaatg ctcgaatcag catgagggaa caactttcgg gcgcaaaaac
    36841 attgccggaa gcgccaccag cgccattacc cagaacacgc cgtggcccag atattgatac
    36901 aggaaaccgg cgaaaacggt catgatagcg atactgccgc ccatcgcgac ggcagagtaa
    36961 accgcctgta aacggatgac ttcgctaccc tggcgagcag caatataacg catggcggcc
    37021 aggtggcaga ccgtgaaggt gccgcaatgc agaatttgca ccactatcaa ccacggcaac
    37081 gccgtagttg ctcccataat gccccagcgc actacgccgc aaatcgccga gatcaacagc
    37141 atatcgcgtg cactacaacg gcggaaaagt ttattactca gcgcaaagat aatgacttcc
    37201 gccaccacgc ccagcgacca caaatacccc accgccgagg ccgagtagcc agctgcctgc
    37261 cagtaaatgg cgctaaaacc gtaataggcc gcatgtgccc cctgcaataa acaaacgcag
    37321 gccagaaagc gccagttctg gcgaaccagc gccaaccacg ctgaccaacc ggtgctctcc
    37381 tgctggcggc ttgccccttg tggctgaatc gtcggacgga tgagaaagcc gagcagcatg
    37441 gatgccacgc ccaacgtcaa cagcgcgagg atcacccgat aatcaaacat agtgaccagt
    37501 ttgcccgtca gcgccgagcc aatgacaaac gccaccgagc cccacagtcg cactttgccg
    37561 taatcaagcg ggaactgctt ttgccacgta ttcgccagtg catcggtcaa cggtaccagc
    37621 ggtgagaaaa agaggttaaa gccaatcatc accagcatca gccacgctac gtgcgccccc
    37681 gcccagaagg cgacagcaaa gagaagtgtc agcagtgcca gcacgcgcaa ggcggaaatc
    37741 aggcgggaag gatcgctgac gcggggcgcg atgagcaaac tcccgagaaa acgggcaacc
    37801 agacctgccc ccaataacag gccgatggtt tctggcgtta aaccaatccc tttaagccag
    37861 acgctccaga aaggtagaaa aatgccgtaa ctaaaaaagt atgtgaaata gccgagcgcc
    37921 aaccagcgcg tggattgcaa aaccatgagt ccctcccgtc aggaggcgtt agtctggggt
    37981 taatcgaatg ttggtgcaag ttgaaaaata agaatattcg agcctgcttc ccgatgtctg
    38041 taacgccagt tcacaagcac taaaaaactg cgatccgcga cgcatgttgc aaaaataatg
    38101 cgtaattatg ccgttacgct tgccaaacgt tcctgcacaa tggcgatgaa atcgcgtaat
    38161 gccggcttca tctccccttt cttccacgcc atcagtaaag caatggaagg cacatttccg
    38221 gcaatgggac gaaaaacaac ctgtccggta ttaaaattat tcatataacc gggtatcaaa
    38281 gtgacgccca gccccatgcc caccagattc atggtcacca gaatattcgt tgccacctgg
    38341 acgatatttg gctggctgtt ttcttgcgca aaccacgctt taacgatcgg cgcaagcgaa
    38401 ccggaatacg ccggatcggt actgacgaaa ttcacgccat ccagttgcgc ggcggtgatc
    38461 tctttttcat gtgctaaagg gtgatcaacc ggtaacacaa ccactaatgg ttcgtcaaaa
    38521 agctccagat aatcaatctc cgggctataa acaggatggc gcattaagcc gacgtcgagt
    38581 tcaccacggc gaattttttc ctcctgttgc gtggtgatta aactcaccag ctcaatcaag
    38641 gtgtctggct gtctgagacg aaacatcggt aatacttttg gcagtaaatt cacttccgcc
    38701 gatggcacaa agccaatggt taattgtctg tcttcctgaa caattttccg cgcccgtaat
    38761 ttggcatttt ccgcttgttc gaggattgcc agcgcgtcct ggagaaaaca ttctcccgcc
    38821 gccgtcaacg cgactttgcg cttatccctc accagtaacg gaacaccgac acagttttca
    38881 agatcgcgga tctggctgct taacgaaggc tgtgaggtat gcagtttttc cgccgcacgg
    38941 gtaaagttca gtgcctgcgc cactgcgacg aaatagcgta aatgccgtag ttccatcacc
    39001 ttccccttgt tatcgaaaaa acgtctcagc tggtagaaat taactatttc acatattagc
    39061 aaccaaccag caacatcctt atggtacaaa aatagaaggt caatacatct tatctttcag
    39121 gattaaaaaa tatgaccaca ccctcagatt tgaacattta ccaactgatt gacacccaaa
    39181 atggtcgggt cactccgcgt atttataccg acccggatat ttaccaactg gagcttgagc
    39241 gtattttcgg tcgttgctgg ctatttctcg cccacgaaag ccagatccca aaacccggtg
    39301 atttctttaa cacctacatg ggagaagatg cggttgtcgt agtgcgtcag aaagacggca
    39361 gcatcaaggc gtttctcaac caatgccgcc accgggccat gcgtgtgagt tatgcagatt
    39421 gcggcaacac tcgcgccttt acctgcccgt atcacggctg gtcttatggc attaacggcg
    39481 agttgatcga tgtaccgctg gaacctcgcg cctacccaca agggttgtgt aaatcccact
    39541 ggggactaaa cgaagttcct tgtgtggaga gttataaagg gctgattttt ggcaactggg
    39601 ataccagcgc accgggcctg cgtgattacc tgggtgacat tgcctggtat ctggatggca
    39661 tgctggatcg tcgcgaaggc ggcaccgaaa ttgtcggcgg cgtacaaaag tgggtgatca
    39721 actgtaactg gaaattcccg gcagagcagt tcgccagtga ccagtatcat gctctgttca
    39781 gccatgcttc tgccgttcag gtattagggg cgaaagatga tggcagcgat aagcgcctcg
    39841 gtgatggaca aaccgcccgc ccggtgtggg aaaccgccaa agatgcgctg caatttggtc
    39901 aggacggtca cggtagcggt ttcttcttta ctgaaaaacc ggatgctaat gtctgggtcg
    39961 atggcgcagt ttcaagctat taccgcgaaa cctatgccga agcagaacaa cgtttaggtg
    40021 aagttcgcgc cctgcgcctg gcgggtcata acaatatttt ccccacgctt tcatggctca
    40081 acggcactgc cacgctccgc gtctggcatc cgcgcggccc tgatcaagtt gaagtgtggg
    40141 cgttctgtat tactgacaaa gccgcctccg atgaagttaa agccgctttt gaaaacagcg
    40201 ccactcgtgc ttttggtcct gctggttttc tcgagcagga tgactcggag aactggtgtg
    40261 aaatccagaa attgcttaaa ggccaccgcg cccgcaacag caaactgtgt ctggaaatgg
    40321 ggcttggtca ggaaaagcgc cgcgacgacg gcattcctgg cattactaac tatatctttt
    40381 cagaaactgc cgctcgtgga atgtaccaac gctgggccga tcttctgagt agcgaaagct
    40441 ggcaggaagt gctcgataaa accgccgctt accagcagga ggtgatgaaa tgagtgcgca
    40501 agtttcacta gagttacatc accgcattag ccagtttctc tttcacgaag ccagcttact
    40561 ggacgactgg aaatttcgtg actggctggc gcagctcgac gaagagattc gttacaccat
    40621 gcgcaccaca gttaacgcgc aaacacgcga ccgccgcaaa ggcgtccagc caccgacaac
    40681 ctggattttt aatgacacca aagaccagct ggagcggcga atcgcccgtc tggaaacggg
    40741 catggcctgg gcagaagagc cgccgtcacg cacccgtcac ttaatcagca actgccagat
    40801 aagcgaaacc gacatcccaa acgtatttgc tgtgcgggta aattatctgc tttatcgggc
    40861 acaaaaagag cgcgatgaaa cattctatgt tggaacgcgt ttcgacaaag ttcgccgtct
    40921 ggaagatgac aactggcgct tgctggaacg ggatatcgtc ctggatcaag cggtaatcac
    40981 ttcccataac ctgagtgtac tgttctgatg aatcgaattt atgcgtgtcc cgttgcggat
    41041 gtgccggagg gtgaggctct ccggatcgat acctcgcccg tcatcgccct gttcaacgtt
    41101 ggcggcgagt tttatgccat taacgatcgt tgcagccatg gtaatgcgtc aatgtcagaa
    41161 gggtatctgg aagatgacgc cacggtggag tgcccgctac acgccgccag tttttgcctg
    41221 aaaacgggga aagcgttatg cctgcccgcc accgatccgc tcaccactta tccagtacac
    41281 gttgaaggtg gtgacatttt catcgactta ccggaggcgc agccatgagc gatctgcata
    41341 acgagtccat ttttattacc ggcggcggat cgggattagg gctggcgctg gtcgagcgat
    41401 ttatcgaaga aggcgcgcag gttgccacgc tggaactgtc ggcggcgaaa gtcgccagtc
    41461 tgcgtcagcg atttggtgaa catattctgg cggtggaagg caacgtgacc tgttatgccg
    41521 attatcaacg cgcggtcgat cagatcctga ctcgttccgg caagctggat tgttttatcg
    41581 gcaatgcggg catctgggat cacaatgcct cactggttaa tactcccgca gagacgctcg
    41641 aaaccggctt ccacgagctg tttaacgtca acgtactcgg ttacctgctg ggcgcaaaag
    41701 cctgcgctcc ggcgttaatc gccagtgaag gcagcatgat tttcacactg tcaaatgccg
    41761 cctggtatcc tggcggcggt ggcccgctgt acaccgccag taaacatgcc gcaaccggac
    41821 ttattcgcca actggcttat gaactggcac cgaaagtgcg ggtgaatggc gtcggccctt
    41881 gtggtatggc cagcgacctg cgcggcccac aggcgctcgg gcaaagtgaa acctcgataa
    41941 tgcagtctct gacgccggag aaaattgccg ccattttacc gctgcaattt ttcccgcaac
    42001 cggcggattt tacgggtccg tatgtgatgt tggcatcgcg gcgcaataat cgcgcattaa
    42061 gcggtgtaat gatcaacgct gatgcgggtt tagcgattcg cggcattcgc cacgtagcgg
    42121 ctgggctgga tctttaagga agcacgatga aagaaaaaac gatcattatt gtcggtggcg
    42181 ggcaagcggc ggcaatggct gcggcctcgc tacgccagca agggttcacc ggtgagctgc
    42241 atctgttttc cgatgagcga catcttcctt atgaacgacc tccactctcg aaatccatgt
    42301 tgctggaaga ttccccgcag ttacagcagg tgttacccgc taactggtgg caggaaaaca
    42361 atgttcatct gcattccggt gtaaccatca aaacgctggg tcgcgacaca cgagagttag
    42421 tgttaaccaa cggcgaaagc tggcactggg atcagctttt tatagcaacc ggcgcggcag
    42481 ctcgaccgct gccgttgctt gatgcactgg gagaacgctg ctttacccta cgccatgccg
    42541 gtgatgccgc cagactgcga gaagttctgc agcccgaacg gtcagtcgtg attatcggtg
    42601 ccggaactat tggtctggaa ctggctgcca gcgccacgca gcgcagatgt aaggtgacag
    42661 tgattgaact ggcggcaacc gtcatgggcc gtaatgcacc accgcccgtg caacgctatc
    42721 ttttacagcg ccaccagcag gctggtgtgc gcattctgct caataatgcc attgaacatg
    42781 tggtcgatgg tgaaaaagta gaactgacgc tgcaaagtgg ggaaacgctt caggccgatg
    42841 tggtgattta cggtattggt atcagcgcca acgaccaact ggctcgcgag gccaaccttg
    42901 atacggtaaa tggtattgtc attgatgagg cttgccgcac ctgcgatccc gcgatctttg
    42961 ccggtggcga tgtggcaatc actcgtcttg ataatggtgc actacaccgc tgcgaaagct
    43021 gggaaaacgc caataaccag gcgcaaattg ccgctgccgc aatgttaggg ctaccgctac
    43081 cgctactgcc gccgccgtgg ttctggagcg atcagtacag tgataactta cagtttattg
    43141 gcgatatgcg tggcgatgac tggctttgtc gtggcaaccc ggaaactcag aaggcgattt
    43201 ggtttaatct gcaaaacggc gtgcttatcg gtgcggtaac gctgaatcag ggacgtgaga
    43261 ttcgctcaat ccgcaaatgg atccagagcg gcaaaacgtt tgatgcgaaa cagctgacag
    43321 atgagaacat cgcgcttaaa tcactgtaac caggacaatt agcgaatatc tcaacgcctg
    43381 gggcgtggcg aggtaccaga gtgtgtatta cgtttaaatc acattatctt gcaaagggat
    43441 tggttatgaa ctcactacgt tattttgatt ttggtgctgc ccgccccgtt ttgttattaa
    43501 ttgcccgtat cgccgtggtc ctaattttca ttatttttgg ttttcccaaa atgatgggct
    43561 ttgacggtac ggtccaatat atggcctcgc tgggcgcacc gatgccgatg ctggcagcga
    43621 ttattgcggt agttatggaa gtgcccgccg cgatattaat cgtgcttggc tttttcaccc
    43681 gtccgctggc ggtactgttt attttctaca cgctgggtac ggcggtgatt ggtcaccatt
    43741 actgggatat gaccggcgat gccgtcgggc caaatatgat taatttctgg aagaatgtca
    43801 gtatcgctgg cgcgttcttg ctattggcaa ttaccgggcc gggggcaatt tctctcgatc
    43861 gccgttagga aagatgccgg atgcggcgtg aacgccttat ccggcatttt ataaattaca
    43921 gccactctac ccgcaacgac atctctgagg ttgttgattc ccccggcgca agcgcaatga
    43981 ggtccccccc ttccgggcga tgatggtcat ccggcgcatg gctcatcggc tccagacaga
    44041 agaaatcaaa cgcatatcct ttatcaaacg ccgggtcgga aacaaagatg aaataacacg
    44101 gtgcaggtgg cgtcgtttct atgatgatgg cataaccctc ctgcggctgc tcgatgcggg
    44161 cctgaccatt ccatccggca aaaccattgt tcacccactg gcgcggcaac ggcgcgagct
    44221 ggttaaaatc cagttcttgc ggtagctgct cgcaaaactc acccgccagc cactgctccc
    44281 gctccagcca gtaaccgctc gcctgtgcct gaatccgcgt ttgcggcgac aacggaaaat
    44341 aaggatgcca gccggtacca aatggcagcg tctctgcccc ttgattggtg acagagagcg
    44401 tcaccgtcag cgtatccgcc gttaaatgaa acgcctgact tacccgatag tgatagacac
    44461 cgctgcgatg ttcatacacc agacacaaac tatcatcgct gtgcgagaca cattgccact
    44521 cgcccagcca gccatcgccg tgtagatagt gcgcatccca ctcaacattc ggttgcagtt
    44581 gatactcacg cccctgccag acaaaacggt tgccgctcac ccggtttgca aatggcacca
    44641 gcggaaaaca tgaggcatcg gttgccacac cgcttttttt accaggacgt aagagcggcg
    44701 tcgtatcgcg ccagaagcct tcgattacgc cgccctggtc agaaacgtcc agctttagcg
    44761 acccgtggga taaggtatag atggtcatcc gctaactcct taatccggga agttaatcac
    44821 aactttcccg catttgccgc tcgccatcag cgcataggca tcacctgcct gttccagcga
    44881 gaagcgatgg gtaatggcgt tacgcggcca cagcttccag tcagtcaaat cgtgggcgca
    44941 tttttccata tggaacagac tggtcaccca ggagccgata atccgccgtt gatggtgcat
    45001 cagatcggcg ctgacctcga attccacttt tccggtttca ccaatgtaaa ccacccgtcc
    45061 ccagtcagcg gtggattgca gtgccagcaa gcgacctgcg gcattaccag aacaatcgag
    45121 cgcaacatcc gcgccaccgt gggtgagttc ggcgataatc tgcggcagac cttcggtggt
    45181 tgctaaatag ccgtgatcca tcacccctaa ctgttttgcc atcgccagac gttccggcag
    45241 catatcaacg ccgatgatcc gttttgcacc gcgacctttc gccagcatca tcgccatcat
    45301 gccgaccggc cccagaccga ccaccagcac gttatcgctg ccggaaactt cgccgcgcaa
    45361 aatcccttca taggccgtgc caacgccgca actgataaac gcaccatctt tgtagctcag
    45421 cgcatccggc aggaggatca gatctttttc ttccgccagc agatattcgg catgaccgcc
    45481 gtcacgctgc cagccgtaag ccgcttttcc tttgccagta caagagatag gaaagccgcg
    45541 gcggcagttc gggcaaaaac cacagccaga aatgtgatac accagcacgc ggtcgccctc
    45601 tttaaaatgg cggcagcctt gccccatcgc cacaatctgc ccgcacggtt catgaccgtt
    45661 gataaatccc tggtataacg gtttatcggg tgccgccgcc gtggcacggt gttgatgata
    45721 gatatagtgg acatcgcttc cgcaaatccc ggaggatttc attttgatca gtacctggtt
    45781 aatccccggc gtcggcaccg caacttcccg cagatcgacg gttgaatttc ctggtaaata
    45841 agctgccagc atcgttttca taaatccctc attaacaata cgattaattt tcatccctgc
    45901 cgcacccgcg ccaggggcgt taaggtttag cgtttcgctt tactgcttcg ctgggtcagc
    45961 aagatattcg ccagcaccgc caccacgatg atgacgccgc gtaccacctg ctggaaaaag
    46021 gagttaatac cgagcagcac cagaccgtta ccgattagcg taatcaccag cacaccaagc
    46081 aatgtaccga acagggaacc gcgaccgccg gaaagtgccg taccgccgac cacgaccgca
    46141 gcgatgacgt caaactccag accgtttgcg gcacctgcgt taccggaacc gaggcgtgcc
    46201 gccagcaaaa tgccggtcac cgccgctaat aatcccgaaa gggtaaagat aagaatgcgc
    46261 acccgacgaa cgttaatacc gcacaactgc gccgccgtgg cattaccgcc aacggcaaaa
    46321 accgagcgcc cgaaggcggt ttttcggcta atgaacacaa acagcgcaaa caacaccatc
    46381 atgatcagcg cggatactgg cacaccgaga aattgtccgc ccagccagtc cagcacctcg
    46441 ttttcgtcaa tcggcactgg cagtgcgttg gtcataaaca gccccattcc gcgcagggcg
    46501 ctccacagcc ccagtgtggc aacgaaactt ggtacgttaa acacgccgcg cagcaccccg
    46561 gccagcgttc ccatcagcgc gcctaacagc aacaccagca gacaagcaat cgccaacgga
    46621 acttcaaatt gcagcaaaaa tgccaggcac accgagacaa aagccaccat cggcccaacg
    46681 ctgacatcaa tttcaccgga gataataatc agcgtcatcg cccaggcggc aatcccaatg
    46741 gtggcggcat cgcgcagcac gttcatctgg ttattcaatg agataaagcc aggcgcgttc
    46801 agggagaaga ccagataaag aatggcaatc accaccagca aaccgatctc attaatatgg
    46861 cgactgacaa attgtttgag cgagacgctc ttgccctgcg gcaatggtaa tgacgaagca
    46921 gacatcttag gttcctcggt aaaattcatc agtgcacaga cagaatggcg gacatcagct
    46981 catccacatt gaccggagcg tgaaactcct gcgagaacgt accgtgctgt aacaacagaa
    47041 tgcggtcgca caccagcggt aactcctcca cttcactgga gataaacacc acgctttttc
    47101 cttcggcagc cagctcacgg acaatacggt aaatctgctg tttggcttcg atatcgacgc
    47161 cgcgcgtcgg ctcgtcgagc aacaaaatct ggctggcggc atagacccaa cgaccgatca
    47221 ctactttttg ctgattgccg ccagaaagcg tgccgatggg cgtttcgcta ctggcggcct
    47281 tgaccgtcat ccgctgcatc acttcttcgg tcaggcggcg gatggtggac cattgcagca
    47341 ctccgttggc gctgattttt tgccgattgg tcagcactgt attttcgtca acgcccaacc
    47401 agggaatgat ccccgcttct ttgcggtttt ctggcgtata gccaatgccg cgtttcagca
    47461 tgtcgccgta atcggggcgc atgattttct cgccgttgat aacaatttcg ccctgttcat
    47521 acgtctccag cccaacaatc gccttcagca attcactgcg ccctgccccc agcaggccag
    47581 caatgccgag cacttcgcca cgacgtagcg taaagctgat atcctccagc ttgggcttat
    47641 ggcgtaacgc acggacttcc agcacggcct gatccacaat ttcctgaggt gcaaccggcg
    47701 caatatcaac gtgatcgcgc cctagcatta gcgacacaat atgatgcgtg gaggtgtttt
    47761 cgagcatcac atcgccagcc acctgcccgt cgcgcataac ggtggcacag gaggcaatgc
    47821 ggcgaatttc ttccatccgg tggctgacat aaatcaccgc cacgcccagt gccgacatct
    47881 ttttcaccgc gctgatcacc agttcaactt ccgcactcgc aagcgaactg gtaggttcat
    47941 caagaatgac cacgcgcggc tcgcccttca tcacccgcgc aatttccacc agctgcttat
    48001 gcgccgggct tagtgttgaa acaagttgtt caggactaac gtcaacgccc agcgcctgta
    48061 agcaacgttg ggcatcctgt gccatttgca ggtaatcaat catgccgttg cggcggggcc
    48121 actgaccgag gcagaggttt tccgccaccg tcagcccttc caccagactt aactcctgat
    48181 aaaccgcgcg tacccccagt tcagcggcac ggcgagtcag cgtagcttcg tcaccttcca
    48241 gtcgcgtctc gccaatccag atttcaccgc tatccggacg ttcactgccg gtaagcattc
    48301 gaatgagggt cgatttgccc gcgccgtttt tacctagcag tgcacgaact tcgcctttat
    48361 tgagcgtgaa gttaacgtta tccaacgcaa cgacgccggg ataacgctta tttcctgcca
    48421 ccacttttgc taccgggact gcctctgttg ccgtgaacat agggacctct gcgaatcagc
    48481 gattagggca gaccatcaac gtgcgttgcc agccactgtt taccgtcttc cgttttggta
    48541 tagagatcga taggcacctg aatcactttt tcaccgtcgg cttgtttatt gataaccttc
    48601 aatgtttgcg cgaaaacagc attgcccatt tttttaccgg aaatatccac taccgctttc
    48661 agcacctggt tgttttccag ctcctgagca atttcagtgg tcatatccga accgaaaacg
    48721 gcaatttttc ctgcctgatt ttgattacgt accgctttta ccgcgccgag tgtcgcaccg
    48781 cccgattctc ccataatggc gttgagatcc ggcgtggaga taattagttt ttcaccaacg
    48841 gaaatcgctt tatctaaaac agtcccttcc tgattagcga caatttgcgc gccgggaacg
    48901 cgggctttta atacttcttc aaacccttta cgtcgttgca cacaaacttc aaaggcttcg
    48961 caattgatga cggcaatttt cggctggtca attttgttgg caataaaata atcggcggca
    49021 gcgttaccca gttttttacc aaattccagc gggtcaccca ccagatatgc cgagacatat
    49081 ttatcgacac ccttttgatt aatacaggtg ttgtagcaaa tcaccggaat acccgctcca
    49141 ctggcgcgac gaacggtacg actactgcca ttttcagaca ctgccgataa aataatggca
    49201 tcgacattac gcgccacgag ggtatcaaca aaggtacttt ctttcgaaat atcaccctgg
    49261 gcgttagttt caattaactg tacctgtact gaagaatctt ttgccgcatc ctgaacgccc
    49321 tgacgcactc cagcgtaata tccctgagta tcaaggtata ttgcgccaat ggtcatttct
    49381 ttttccgcag ccctggcaaa tagtgcgcta cctaatagcg tagccattaa taatagatta
    49441 cgggttgttc tgatttttgt aggcatagag cctcctgtag ggtttttatt aacaacggct
    49501 tattctaatt attttgtgat gagcggcagc gcgtgccgct caaatattac agttaatagg
    49561 gtttagtgaa cgtagttaaa aataaaaggc atcacggtgg tgaataatgc cgctttcggc
    49621 caggccggat taatcgccgt caattcagcg cgagcagact catattgcgc aacctccccg
    49681 agtcccgcgg acgccagcat cgccaccatc aggcattttt ctttatgctg ctgttgtaaa
    49741 tcgccataca gcgataacag gtcaggttgt gaaacagcaa agaaatccgc ctcaatactg
    49801 gttttcgcca tctcttgcgc ccactgtttc atttcactaa acagttgctg tgcgatttgt
    49861 tgttcgccca gcagtcgcag cgccattcct tgccagaaga gataatcaac cggctgatcg
    49921 ttgtaataac tgtggatgtt aatggtgcga tcgccggtcg ccgccagacg taaacaacgc
    49981 gtcgcttcag tttcatcgcc ctgcgcgttg gcgcatatcg cctgccagaa ccagatgtcg
    50041 ttatcagttt gccccggtaa acggccttcg cttaaattct ccggataatg cagcgcggca
    50101 tgaagcagtt cgctggcctg ctgcggctgt ctggcatcaa gatgctgcca ggcgcgtaat
    50161 aactggttga ggataaactg actggtgacc ttcccttccc cgccttccca cgggtggaat
    50221 ttgcgcgtgg cgagaatgtc cgccgctttg tctgcctgcc ccgtgagatg ccacaaattg
    50281 agcagttctg cggtcatgtc gtcgcgtttc agcgcaattt ccagattatt ttccagacgc
    50341 gccagtcgtt tctccggtgt ggctccactt aacttatcaa gcaaatcccg ttcgaaaagc
    50401 agacgtgcat cctgcggcgc aagctgataa gcattatcaa gataacgcgc ggccagctca
    50461 taatcgtgtt gcttattcca cgcatggatc gctaacccgc gccagccgtc ggcaaactcc
    50521 ggcgacatct ctacgcaacg ttgccataag gcaatggctt tgttgtagct acgtttgttg
    50581 tagtagaagc aggccagtaa atggcgagca aaccagcact cttcaatact ctccagcgcc
    50641 gccacttctt ccagcgtatt cgggaaacgg acaaactgcg ggaagacatc aatggctttt
    50701 gcgaccagtt cgccacgttc ggctttcggc agcaagctgg cttgcaggta aagcggcagc
    50761 gtgcgctggc agtccagtgc gttcagcatc tctgccgcca gggtgggcat tccccagtta
    50821 atcagttgcc cggcggtcat cagggcgtta acgtcgcgtc cctgacacag accgcgccac
    50881 tgcgcgagag ctgattcgct acgaccatcg aaccagttca gccaccacag agtggcgttc
    50941 agcggataat cgcgcagcag tttctcgcgc tgcaaacgcg cgttgtcctg acaaccactt
    51001 aacaccagca gcagattatg caggcaaagc acttcctgat tggttgggca ggcgcgaaga
    51061 ctttgttggc aaaaatccag acccgcgtcg aagttaccat tacgcgccgc cagtcgtgcc
    51121 agaccataat agcctccggc tttactgttg ccgctccaga ccgcacgcca gaaatcctct
    51181 tcggcttgtt gatattgtcc ctgacgttcg taagcactgg cgcgaatcaa actcgcctgt
    51241 ccgcactgcg gatttttgtt cagcgcatgt gcgcgtttca gagcctgagt ggcatacgcc
    51301 accgcttgcg ggaaatctgc gcggttatac tccagcatcg ccagcgccag attacagcgg
    51361 taatccagcg gatccagcgc cacgccgcgc aggtagtaat cgaacggtga acggctggcg
    51421 tgatgatatt gctccagatg ctgaccgata aaccaggctt catctgtact ggtaatgtct
    51481 tgtgctgcca gtggcgcttt ggcgacgtcc ggcaacggca actcttgcgg ctgatgttcc
    51541 tgataactca gtacaatatt gccgtcggca tcggagagct caatggtcag cctttcagga
    51601 ttgataccgt gcaacacgcc ctggatggcg gtcgctggtg tcagggccac ggcatcatcg
    51661 agcaacgcgt cgcatttgcc gatttcgcgg atcgccaggc ggtatccgtt caacggagag
    51721 atggcataca gcccccactc aatcccccgc tcactacgct ggagtttgat taccgcatcg
    51781 cgggaggcgt tttgcaccat gcccagagaa tggtaaggca ggaaatactg ctcaaaacgc
    51841 ttctcttcgt aagcatcaag ccaggtaaaa tcaggctggt tatcggcaaa aataccggtc
    51901 atcagttcga tatacgggcc gttattgtcg gtcagactct tatcccacgc ctggccaaat
    51961 tcactgtgtc cccaactcca ctgtttttta cctggcgcaa tatggtggtt ggcaacgtgc
    52021 agcaaaccgc catcttcatc gtgacaccac gcgccgacaa aatcgtactg cgatttttcg
    52081 gccatatatg aggttggaac aggcacattt ttatagcgag aaatgtccac tccagcggag
    52141 tagtccactt tgtagtaagt gccggtggcg atagggaaag cggagacggc ccgtttgccg
    52201 tgatcaaaca ccgccgttac atccggcggg aagacgctct gatgcccttc cccccctttc
    52261 actgccgggt tggcccacca caagaaatga cgcggcgtgg cgttgccgtt atagacgcgg
    52321 ctggcgattt ccagcgccgc ccggtcaggg cgcagggtga aacctgtcat cacctgtaaa
    52381 ccatgcatcg gctccgtttc gcctacccac accgtctgtg cgccgtctcc atgggcttcg
    52441 agggtgaaat caacgggcat aaaggtggtc gggcgatggt gttgcggcca gttaaactca
    52501 atcccgccgg agatccacgg tcccagcagc cccaccagcg caggtttaat gacttcattg
    52561 tgatagacaa aatcgcgctg tttcacttta tcccatgcgc gatgcacccg accgcccaat
    52621 tccggcagga tcatcacttt gatgtagtcg ttttccagcc acaccgcctg ccaggatttc
    52681 agggttttct gctcgctcag cgtatcggtc acgccgtagg gataaacagc gccggacgat
    52741 ccctgataaa cgcgattttc gaggaacatg ggatgtatat cctgcggccc ggtttcatag
    52801 gtcgggatct caacgcgctc ttgccacact tttactggag tcatggtgcc ctcaatatta
    52861 acaagacata ctgaattaaa agatttgtgg cagtgtattg aacaatctgg caatgttttc
    52921 gcggaataat cacgcaatta actaaacaag gtttagtgaa gatgagagcc tgcattaata
    52981 atcaacagat tcgccaccat aacaaatgcg tgattctgga actgctgtac cggcaaaagc
    53041 gcgccaataa atcaacgctg gcccggctgg cgcaaatttc gattccggca gtcagtaata
    53101 ttttgcagga actggaaagc gaaaaacggg tggtgaatat tgacgatgaa agccagacgc
    53161 gcgggcatag tagcggtaca tggctgattg cgccggaagg tgactggacg ctgtgcctga
    53221 acgtgacgcc caccagtatt gagtgtcagg ttgctaatgc ttgtttaagt ccgaaaggtg
    53281 aatttgagta tttacagatt gatgcaccga cgccgcaggc gctgctgtcc gaaatcgaaa
    53341 aatgctggca tcgccaccgt aaattgtggc cggaccgcac catcaatctg gcgctggcaa
    53401 tccacggtca ggttgatcca gtgacaggag tgtcgcaaac catgccgcaa gcgccgtgga
    53461 caacgccggt tgaggtgaag tatctgctgg aagagaagct cggcattcgg gtgatggtcg
    53521 ataatgactg cgtgatgctg gcgctggcgg agaaatggca aaataattcg caggaacggg
    53581 atttctgcgt gatcaacgtt gattacggca ttggctcgtc gttcgtgatt aacgagcaaa
    53641 tttatcgcgg cagtttgtat ggtagcggac agattggtca caccatcgtt aatccggatg
    53701 gcgtcgtctg cgactgtgga cgttacggct gcctggaaac cgtcgcctcg ttaagcgcat
    53761 taaaaaaaca ggcgcgggta tggctaaaat cacaaccggt taatactcag cttgatcctg
    53821 aaaaactgac aaccgcgcag ttaatcgctg cctggcagag tggagaaccg tggatcacca
    53881 gctgggttga ccgcagtgcc aatgccattg gtatgagtct gtataacttc ctcaacatcc
    53941 tcaatattaa tcagatttgg ttgtacggtc gcagttgtgc ctttggtgag aactggctta
    54001 atactattat tcgccagaca ggatttaacc cgttcgaccg cgacgaagga ccgagcgtga
    54061 aagcaacgca aattggcttt gggcaattaa gccgcgcaca acaggtgctg gggattggct
    54121 atttgtatgt tgaggcgcag ttacgacaga tttgatggcg cgataacgta gaaaggcttc
    54181 ccgaaggaag ccttgatgat cataaacgaa aaattgcctg atgcgctacg cttatcaggc
    54241 ctacacggag attgcaatat attgaatttg caagattttg taggcctgat aaggtgttca
    54301 tgccgcatcc aacatgaaca acgagcacat tgtcagcaaa tcaccgtttc gcttatgcgt
    54361 aaaccgggta acgtgcgcag atgtcgagaa ctttaccttt gatgcgctcg ataacggctt
    54421 catcattgat gctgtccagc acgtcacaca tccagccagc cagttctttc gcttctgctt
    54481 ctttgaagcc gcgacgcgta atcgccggag tacccacgcg aataccggag gtcacaaacg
    54541 ggctcttcgg atcgttcggt acgctgtttt tgttgacggt gatgttagca cggcccagag
    54601 cggcgtctgc ttctttaccg gtcaggtttt tatctaccag atcaaccagg aacagatggt
    54661 tatcagtacc gccggaaacc actttgtagc cgcgttccag gaacacttct accatcgctt
    54721 tagcgttttt agcgacctgc tgctggtaag ttttgaactc aggctccatc gcttctttca
    54781 gagcaaccgc tttaccggcg attacgtgca tcaacggacc gccctgacca ccagggaaaa
    54841 cggcagagtt cagttttttg tacagctctt cgctaccacc tttcgccagg atcaggccgc
    54901 cgcgcggacc cgccagggtt ttgtgagtgg tggtagtaac aacgtgagca tgaggaaccg
    54961 ggttcgggta gacgccagca gcaaccaggc ccgcaacgtg cgccatatca acaaacaggt
    55021 aagcaccgat gctgtcagcg atttcacgca ttttcgccca gtcaaccacg ccggaatatg
    55081 cagagaaacc accgataatc attttcggct tgtgttcttt ggcttgtttt tccagatcgg
    55141 cgtagtcgat atgaccggta gcatcaatac cgtaaggaac gatgttgtac agtttaccgg
    55201 agaagttaac cggagaaccg tgagtcaggt gaccgccatg cgccaggttc atacccagaa
    55261 cggtatcacc tggttccagc agcgcggtgt agaccgcaaa gttagcctgg gagccggagt
    55321 gcggctggac gttagcgtag tcagcgccga acagttcttt cgcacgatcg atcgccagtt
    55381 gttcaacgat atcaacatac tcgcaaccgc cgtagtagcg tttacccgga taaccttcag
    55441 catatttgtt ggtcagctga gaaccctgcg cctgcattac gcgcgggctg gtgtagtttt
    55501 cggaggcgat cagttcgatg tgctcttcct gacgtacttt ttcctgctcc atagcctgcc
    55561 acagttcggc atcataatcg gcaatgttca tttcacgctt taacatccgc atctcctgac
    55621 tcagctaaca ataaaatttt tggcctttat aggcggtcct gttggacaac ggcgaacagt
    55681 ataaccgaat cattgtgcga taacaggtct tgacaaagga atttacgcaa acgattacct
    55741 tcaggctacg caaggctttg gagaataaag agcttgcaac cggaaacgga tttcttttca
    55801 ggtttgtgat gcaaattttt cacttcatca cattctttct gaaaaacacc aaagaaccat
    55861 ttacattgca gggctatttt ttataagatg catttgagat acatcaatta agatgcaaaa
    55921 aaaggaagac catatgcttg acgctcaaac catcgctaca gtaaaagcca ccatcccttt
    55981 actggtggaa acggggccaa agttaaccgc ccatttctac gaccgtatgt ttactcataa
    56041 cccagaactc aaagaaattt ttaacatgag taaccagcgt aatggcgatc aacgtgaagc
    56101 cctgtttaac gctattgccg cctacgccag taatattgaa aacctgcctg cgctgctgcc
    56161 agcggtagaa aaaatcgcgc agaagcacac cagtttccag atcaaaccgg aacagtacaa
    56221 catcgtcggt gaacacctgt tggcaacgct ggacgaaatg ttcagcccgg ggcaggaagt
    56281 actggacgcg tggggtaaag cctatggtgt actggctaat gtatttatca atcgcgaggc
    56341 ggaaatctat aacgaaaacg ccagcaaagc cggtggttgg gaaggtactc gcgatttccg
    56401 cattgtggct aaaacaccgc gcagcgcgct tatcaccagc ttcgaactgg agccggtcga
    56461 cggtggcgca gtggcagaat accgtccggg gcaatatctc ggcgtctggc tgaagccgga
    56521 aggtttcccg catcaggaaa ttcgtcagta ctctttgact cgcaaaccgg atggcaaagg
    56581 ctatcgtatt gcggtgaaac gcgaagaggg tgggcaggta tccaactggt tgcacaatca
    56641 cgccaatgtt ggcgatgtcg tgaaactggt cgctccggca ggtgatttct ttatggctgt
    56701 cgcagatgac acaccagtga cgttaatctc tgccggtgtt ggtcaaacgc caatgctggc
    56761 aatgctcgac acgctggcaa aagcaggcca cacagcacaa gtgaactggt tccatgcggc
    56821 agaaaatggc gatgttcacg cctttgccga tgaagttaag gaactggggc agtcactgcc
    56881 gcgctttacc gcgcacacct ggtatcgtca gccgagcgaa gccgatcgcg ctaaaggtca
    56941 gtttgatagc gaaggtctga tggatttgag caaactggaa ggtgcgttca gcgatccgac
    57001 aatgcagttc tatctctgcg gcccggttgg cttcatgcag tttgccgcga aacagttagt
    57061 ggatctgggc gtgaagcagg aaaacattca ttacgaatgc tttggcccgc ataaggtgct
    57121 gtgatttgat gttgccggat ggaaacatcc ggcaaccctt gacgcggttt aaattgccgc
    57181 gtcgtcctct tcaccggtac ggatgcgaat gacccgtgcc acgtcaaaga cgaagatttt
    57241 accgtcaccg attttgccgg tttgcgccgt gcgaataatg gtatcgacac aggtatcgac
    57301 aatgtcgtcc ggtacgacaa tctcaatttt cactttcggc agaaaatcca ccatatactc
    57361 cgcgccgcgg tacagctcgg tatggccttt ctgacggcca aaacctttca cctcagtgac
    57421 cgtcatgccg gtaatgccga cttcagccag tgcttcgcgg acatcgtcca gcttgaaggg
    57481 tttaataatc gcatcaatct ttttcatgct attccttgaa aaggtcgcct gtcttttgat
    57541 ctgctaaacg taacacataa cgccaattca ttccttgaaa tcgtttgcat ccagctcgtg
    57601 tcgggaaagc agtttataaa attctgtccg gttgcgcccc gccattctcg ccgcgtgggt
    57661 gacgttgcct ttggtaattt gcagcagctt acgcaaatag ttgagttcaa actgattacg
    57721 tgcctcaaca aaggttggca gcgccgtgtt ttcgccctcc agcgcctgct ctactaatgc
    57781 atcactaatc accggagatg aggtcagcgc cacgcactgt tcaatcacgt tgaccaactg
    57841 gcgcacatta cccggccagc tcgcggtcat caggcgtttc atcgcatcgg tagagaacgc
    57901 gcggacaaac ggtttatgtc gctctgccgc ctggcgcaac aggtgatttg ccagtagcgg
    57961 aatgtcttct gtgcgctccg ccagtgccgg aattttcaag ctgacaacgt tgaggcggta
    58021 atagagatct tcgcggaatt ccccgcgcgc catcgctttt ggcagatcac ggtgagtggc
    58081 agaaataatc cgcacattga tatcattatc gcggttactg cccagcgggc gcactttacg
    58141 ctcctgcaac acgcgcaaca gtttgacctg caacggcgca ggcatatcgc caatctcatc
    58201 gagaaatagc gttccgcctt ccgccgcctg gaataaacct tcgcgattgc tgacagcgcc
    58261 agtaaacgcg ccacgcgcat gaccaaacag ctccgactcc agcaattgct caggcaatgc
    58321 accacagtta atagcaataa atggtttgct attgcgcggg ctggcgttgt ggatagcctg
    58381 ggcgaaaatc tctttcccgg tgccgctctg accgttaatc aaaacgctaa cgtctgattg
    58441 cgccaccagc cgcgcctgtt ccagcaaacg cagcatcagc gggctgcggg tgacaatcgc
    58501 ctcgcgccag cgttcgtcgg tggctggcgc ggattgctcc agcgcatcgt caattgcctg
    58561 atatagcgca tctttatcga caggcttggt gaggaaactg aacacgccct tctgtgttgc
    58621 agcaacggca tcgggaatag aaccatgcgc ggtaagaata attactggca ttcccggctg
    58681 cactttctgg atttcagcaa acagctgcat accgtccatt tcatccatcc gcaggtcgct
    58741 gatgactaaa tctacttttt cgcgattcag tacccgtaat ccttcagcgc cactttccgc
    58801 cgtgaccaca ctgtagcctt cgctggtcag gcgcaggcca agcagtttca gcaatcccgg
    58861 atcgtcatcg accaataata aatgcgcagg tttatggctc atcaggagtg acctcatggg
    58921 tggatggcgc gggcttttca ccctcgtgcg gcgtatccgg cgagaaattt ccagccggtt
    58981 tgcgggtcga gagctggcgt tcaatatcgg tcaggttttc cagcttgcgg gtggtgagtt
    59041 ccagctgctg ttgtagaacg tgatgttgct ggcgcaaagt atccagctcg ctgtcactgg
    59101 actgctggag tttgctgtag cgttggcgct cttccgccag ttgcagttgc agcgcctgac
    59161 cgtcgcgcca gagttgatac agagggcgaa cctgtgccgg gatctcggta cttagcgcat
    59221 caatgcgcgc gaccagctgg cggcgctcat acggcgtaat tttggcgtcg gcgagcaaaa
    59281 tcccttgttt aaaggtattt tgccagctgc cgtcgtcata ttggcgagct tgctgacgcg
    59341 actgcgcagg cattaaacga tcagcacaat ccatcgcccg cagccagtaa agcggattgg
    59401 tttcggttga tttaccttgc agcgcccaga tgtcgctaca ttcagtagaa agatagtcag
    59461 ccagttgata aaccggaatt ttttcttctg ctggcgtatc aatggctggc ttattgtgat
    59521 tctgcacgca acccagcaat gccagacatg gcagccctgc cagccacagc cgtcggggca
    59581 ataatcgttg aaaaatgtgt cgcatattca ccagacttaa agcctatccc agtgggcgta
    59641 attgttgcag acagtttgga catggacagc gcggagaaac cggagcgtac atgtcgtacg
    59701 tgaggatttc gagcactgcc cggggccgaa ctgacaaata aaatagcctg atgggatagg
    59761 ctgttagcat tatttcgtgt ttttcgacga cggtaattca atgcggaaac aaacgtcttg
    59821 cccgctctcg tcgaccagat acagttcccc ttgcatacgg cgaatacaat ccctggcaat
    59881 gcttaatccc agaccgctgc ccttcaccgc cccttttcgc tggtggcttc cctgaaaaaa
    59941 gggttcgaag atcatggcgc gttcctcttg cggaatgggc gtgcctgtat tgatgacatc
    60001 aatataaacc cgcgcaccat gtaaactgct gcgaaggcaa atgttaccgg attcagcccc
    60061 gtagtgcacc gcattggagt aaagattatc cagtacgctc atcagcagca ttggctccgc
    60121 caggcaagct gttgctttga gatcgacgtc ggtatgcatc attttagccc gtgcgggcag
    60181 gctatgagca gaaactactg tttccaccag cggtgctaac tcaacattat ccagttccac
    60241 cgcactgtcc gcctgtttac ggttgtaatc aagcagttgt tcgatcagtt tttgcaaatt
    60301 gcggctgctg ctatcaagaa tgctcaccac ctctttttgc tctggcgtaa gcggcccaac
    60361 aacctggtca gccagtaatt cagtgccctc gcgcatactc gccagtggcg tttttaattc
    60421 atgagataaa tgtcttaaaa attgatggcg ttgggattcc agccatgaca ggcgctcact
    60481 taaccaaaga atacgttgcc caaccgagcg taactcgctc ggtccactga acgaaacgct
    60541 attgcccaga gaacgccctt cccccagacg gttgatcatg cgctcgatat ttttcaccgg
    60601 cccgataatc atccgcgtga aaagtagcac catcaccaga ctcaccagaa atagcaccag
    60661 cgattgccaa ccaaaatatt gcccacgttc ggcgatttca cgctgaagtt gctgcccacg
    60721 agagaacacc actgtgcgcg tggcctgcac catttcggta ttggcactgg caaaggcttc
    60781 aagacgcgcg gcggcggcag catcgggacc actgttgtta cactgaagtt gggccagatt
    60841 gttcaaatcc tgacgcaatg cctggtaaag tttatcgtct ggcagtacgc ctgcgtgggc
    60901 atcgagcatt tcgctgtaac gcttgcgctg gctttgataa accttcgcaa gcgtagggtc
    60961 atccagcacg caatactgac ggtagctacg ctccatctcc agcgccgcgt tggtcattgc
    61021 ttcgctgcgc cgggcatcga taagcgtggt gcggttgacc agcgccgcct ggtcgctaag
    61081 cgcattcagg ctttgccagg cttgccatgc cagcaccaac aggggcagca gaatcagcaa
    61141 aaatgccagc attaccagtt gtcgtaatga gcggggaaaa acgggccagc gtttcaaggt
    61201 gttactctcg tcagacgcga atagcctgat gctaaccgag gggaagttca gatacaacaa
    61261 agccgggaat tacccggctt tgttatggaa taaggcggtg cctaactcga cgtttcgccc
    61321 gatggttgat atagctacgc tgatatcaga agttggacgg caggcacctt gttgtgcgtc
    61381 attcgaattt tatgtagcac gtcccgaagg ggctgacata agtcggtgaa tgagccactg
    61441 gttactatta tgcagtaact gtgccaataa agaaaatagt ttggtaacgt actgattata
    61501 cgctgctttg aggggtttat ggcttcttcg ctatttgaat gtaaatcagc cactgtgtcg
    61561 ctaaaaagag acaacttaag ataaacttat tagatgacat taaaatcaat gagttaaatg
    61621 tcaccaaaaa gcgacacagc aaaccaccca ttgtcgtgat ttacagacac aaaaaagctc
    61681 cctgagttgg gagcttataa tagcggttgg tatttaatgc cggatgcggc gtgaatgcct
    61741 catccgggct gcaaaaccaa tgggctgacg acttacccca actgcttacg tgcattgcgg
    61801 aaaatgcgca tccatgggcc atcctcgccc cagttttccg gatgccagga gttgctgaca
    61861 gtacggaaaa cacgttccgg gtgcggcatc ataatggtga ctcgaccgct ttcagtcgtg
    61921 accgccgtaa taccgttcgg agaaccgttc gggttagccg ggtaggtttc agtgactttg
    61981 ccgaagttat cgacatagcg cagtgccacc aggcctttgc tttccagtgc cgccagatgc
    62041 gccgcatcac gcacttccac gcgcccttca ccatgagaga cagcaatcgg catttgcgag
    62101 cccaccatcc cctgcaatag cagcgacggg ctttgggtca cttcaaccag gctgaaacgc
    62161 gcttcaaagc gatcggaggt attgcgcaca aaacgtggcc acaactcact acctgggatc
    62221 agttcacgca gattagacat catctggcaa ccgttacata cccccagcgc cagcgtttgc
    62281 ggacggtgga agaaggttgc aaactcatcg cgtacacggt cattgaacag gatagacttc
    62341 gcccaacctt caccggcacc cagcacatca ccgtaggaga aaccaccgca cgcgaccagg
    62401 gcgtggaaat cctccaggcc cgtgcgtcca gccagcaggt cgctcatatg cacgtcgata
    62461 gcatcgaagc ctgcacggtg gaaagctgcc gccatttcaa catgcgagtt cacgccctgc
    62521 tcacgcagca cagcaacttt cggacgtgcg ccagtggcaa tatacggtgc tgccacatct
    62581 tcgttgatat cgaacgacag ttttacattc aggcccggat cggcgtcgtt tgatttcgcc
    62641 tggtgctcct ggtcagcaca ctccgggttg tcacgcaggc gctgcatctg ccaggtagtt
    62701 tctgcccacc agacacgcaa cgtagtgcgg ctttcgctga atacagtctg cccgttggcg
    62761 gtaatcacaa aacggtcacc ggaaaccgcc tgcccaacat aatggacgca atcagcaagc
    62821 ccatgctgtg ccagtacgga ctcgaccgct tcacggtcag cggcacgaac ctgaatcacc
    62881 gcacccagtt cttcgttaaa caacgccgcc aggcgatcgt cacccagagt ggcgatatcc
    62941 gcgtcaatgc cacaatgacc agcaaaggcc atttccgcca gcgttaccag caggccgcca
    63001 tcagagcggt cgtgatacgc cagcagctta cgctgtgcaa ccagcgcctg aatcgcgtca
    63061 tagaagcctt tcagttgcgc gacatcgcgt acatctgccg gtttgtcgcc aagctgacga
    63121 taaacctgcg ccagcgccgt tgcgcccagc gcgttattgc ctttgcccaa atcaatcagc
    63181 agcagtgcgt tatcttcggt agaaagctgc ggcgtgatgg tgtgacgcac atcttccacg
    63241 cgggcaaatg cagaaatcac cagcgacagc ggcgacgtca tttcgcgctc ttcgttacct
    63301 tcctgccagc gggttttcat cgacatagag tctttaccca ccgggatcgt caggcccagc
    63361 gccggacaaa gctcttcgcc cacggcttta acggcttcat acaggcccgc atcttcacca
    63421 gggtggcctg ccgccgccat ccagttggcg gaaagtttaa tgcgtttgat atcgccaatc
    63481 tgtgttgcgg caatattggt taacgcttca ccgaccgcca gacgggcaga ggcggcgaaa
    63541 tccagcagcg caaccggcgc acgctcgcca atcgccatcg cttcaccgta gtagctgtcg
    63601 aggctggcgg tagtgaccgc gcagttagcg accggcacct gccacggacc caccatctga
    63661 tcgcgcgcta ccatgccagt tacgctgcgg tcaccgatgg tcaccaggaa agttttctcc
    63721 gccacggtcg gcagatgcag tacgcgtttc accgcgtctg caatggtgat cccttcacgg
    63781 accagcgcat cgcccttcgc tttcagcgtt tgtacatcgc gggtcatctt cggcgtttta
    63841 ccaagcagga cgtccagcgg cagatcgatc ggctgattat caaaatgacg atcgtgcaga
    63901 gaaagatgca gttcttcggt cgcttcacca atcaccgcgt agggtgcgcg ctcacgctta
    63961 cacagttcgt caaacagcgg taattgatcg gcagcaaccg ccagcacgta gcgttcctgg
    64021 gattcgttac accagatttc cagcgggctc atgcccggtt cgtcgcttag aatatcgcgc
    64081 agttcaaatt taccgccgcg cccgccgtcg ctcaccagtt ccggcatcgc gttagaaaga
    64141 ccgccagcgc cgacgtcgtg gataaacagg attgggttgg catcaccaag ctgccagcaa
    64201 cggtcgatca cttcctggca gcgacgctcc atctccgggt tgtcgcgctg tacggaagca
    64261 aagtcgaggt cggcatcaga ctgacctgac gccatggaag acgccgctcc gccgccaaga
    64321 ccgatgttca ttgccgggcc gccgagaacg accagtttcg caccgacgtt gatctcgcct
    64381 ttttgtacgt gatcggcgcg aatgttgccg atcccgcccg ccagcatgat cggtttgtga
    64441 taaccgcgca gctcttcgcc gttgtggctg ttcacttttt cttcataagt acggaagtag
    64501 ccgttcagtg ccggacgacc aaattcgttg ttaaacgccg cgccgcccag cgggccttcg
    64561 gtcatgatat ccagcgcggt gacaatgcgc tcaggcttac cgaaatcttc ttcccacggc
    64621 tgttcgaagc caggaattcg caggttggaa acggagaaac caaccaaacc cgctttcggc
    64681 ttcgcgccgc gcccggtggc accttcatcg cggatttcac cgccggaacc ggtcgccgcg
    64741 cccggccacg gagaaatcgc cgtcgggtgg ttgtgagttt cgactttcat cagaatatgc
    64801 gccggttcct gatggaaatc gtagcggccc gtttcgtggt cagcaaagta gcggcccact
    64861 tcagaacctt ccattacggc ggcgttatct ttataagcag agagaacgtg atctggcgtg
    64921 gtttcgaaag tatttttgat catcctgaac agcgatttcg gctgttgttc accgtcgata
    64981 atccagtcgg cgttaaaaat tttgtggcgg cagtgctcag agttcgcctg agcgaacata
    65041 tacagctcga tgtcgttcgg gttacgacca agctttgtga aagcatcctg cagatagtca
    65101 atttcatctt ccgccagagc caggccaaga cgcaggttag cgtcgatcag cgcctgacgg
    65161 ccctgcccca gcaaatcaac gctggtaacc ggagtcggtt gatggtgggc aaacaactgc
    65221 tctgcatcat ctaaagcaaa aaagaccgtt tccatcatgc ggtcgtgcag ttcagcggta
    65281 acctgctgcc attgttcatt ggtcagcgta ccggcttcta tatagtaagc aacgccgcgc
    65341 tcaaggcggt ttacctgttg tagcccgcag ttatgagcaa tatcggtcgc tttcgaagac
    65401 cagggagaga tggtgccagg acgcggggtc accagcagga gtttgccttg cggggcgtgg
    65461 ctggcgagtg ccgggccata tttcagcagg cgttcaagtt gtgcgtgctc atcatcgttt
    65521 aacggcgcat tgaggtcagc aaaatggaca tactcggcgt aaatattgtg aaccgggagc
    65581 ctggcagcct gaaaacgtgc cagcagtttg ttgattcgga atgccgacaa tgcaggcgaa
    65641 ccacgcagaa tttccatcat aagtctctcg tcttctaagc tttcggtgta cccaaggggg
    65701 ggaaacgggc gtcattataa agaatctgat gcgctgacga aaccgtttgc gtggaaataa
    65761 aatcaccatc gtgaattagc aacgcgtgcc gccaatggct gtaataagtt gccatctggc
    65821 gcaggtttac gcaaaatgcc gctcatttat gagtaaacct ttcactatta ttacgttttt
    65881 tcaagctggg acgcgcacga cacagagaat taactaattg aaaaaattaa agattaatta
    65941 tctgttcatc ggcattctgg cactgctgct cgcggtcgct ctctggccat ccattccctg
    66001 gtttggtaaa gccgacaacc gtatcgccgc cattcaagcg cggggagagt tgcgtgtgag
    66061 caccattcat actcccctga cttataacga aatcaacggg aaaccttttg gcctggatta
    66121 cgaactggcg aaacagtttg ccgattacct cggtgtaaaa ctgaaagtga ccgtgcggca
    66181 gaatatcagc cagctgtttg acgacctcga taatggtaac gccgacctgc tggcggcagg
    66241 acttgtctat aacagtgagc gggtaaaaaa ttatcagcct ggccctacct attattccgt
    66301 gtcacaacaa ctggtttata aagtgggtca gtatcgccca cgtacgctgg gcaacctgac
    66361 ggcggagcaa ctcaccgttg caccgggtca tgtggtggtt aacgatctcc agaccctgaa
    66421 agaaacaaaa ttcccggaat taagctggaa ggtagacgac aaaaaaggct ctgcggaatt
    66481 aatggaagag gtcatcgaag gaaaactcga ttacaccatt gctgattctg tcgccatcag
    66541 cctgtttcag cgcgttcacc cggagctcgc cgtagcgctc gatatcaccg atgaacaacc
    66601 ggtgacctgg tttagcccgt tagatggcga taataccctt tccgccgccc tgctcgactt
    66661 cttcaacgaa atgaatgaag acggtacgct ggcacgcatt gaagagaaat acctggggca
    66721 tggcgatgat tttgattacg tcgatacgcg cacattttta cgcgccgtcg atgcggtact
    66781 gccgcagtta aagcccctgt ttgagaaata cgccgaagaa attgactggc gtttgctggc
    66841 cgctattgct tatcaggaat cgcactggga tgcacaggcc acttcaccga cgggtgtgcg
    66901 cggcatgatg atgttaacca aaaataccgc gcaaagcctc ggcattacgg atcgtaccga
    66961 tgccgaacag agcatcagcg gtggcgtgcg ttatttgcag gatatgatga gtaaagtgcc
    67021 ggaaagtgtg ccggagaacg agcggatctg gtttgccctc gctgcgtaca atatgggcta
    67081 tgcgcatatg ctggatgccc gcgccctgac ggcaaaaacc aaagggaatc ctgacagttg
    67141 ggctgacgta aaacagcgtc tgcctttact tagccagaaa ccctattaca gcaagctgac
    67201 ttacggctac gctcgtgggc atgaagccta cgcttatgtc gaaaatattc gtaagtatca
    67261 gattagcctg gtgggttatc tgcaagagaa agagaagcag gctacagaag cggcgatgca
    67321 actggcgcag gattatccgg cggtatcgcc tacggagttg ggcaaagaga aatttccttt
    67381 tctctcgttt ctttcccagt cgtcatcaaa ctatttgacc cattctccct ctctgctgtt
    67441 ttccaggaaa gggagtgaag agaaacaaaa ttaatccgtc gaggattgcg cttttttctg
    67501 cgctttaatt tcctggcggc gcatgcgaaa gaagtcactg agcaacgccg cgcactcatc
    67561 cgccagtatt ccttccgtaa tttccactcg gtgattcata cccggatgat gcagcacatc
    67621 cattaaagat cccgcagcgc cagttttcgc gtcacgcgca ccaaagacca cgcgaccaat
    67681 gcgactgtgg atcatcgctc cggcacacat tacacatggt tcaagcgtga catacaacgt
    67741 ggcgtcgatc agacgataat tttgcatcac cagaccgccc tgcctcaggg ccatgatttc
    67801 tgcatgtgcg gtgggatcat gtcgaccaat cggacggttc cagccttcgc caataacacg
    67861 attgttatgc actaataccg cgccgaccgg tacttcccgc tcatcccagg cacgcttcgc
    67921 cagcgtcatt gcatgacgca tccagtattc gtggctaaat tcgacttcag acaaaaagaa
    67981 aactccggtt ataaaagcgc ggcgcattat acacggacgc tatgctttac tctattccag
    68041 ttgctggagt tcaccgcgcg gggttactcg ccaacgatgc tgacagaaat aaagcagcgg
    68101 attgtcctga ttactgtcgc tatagccact gtacagccgc agcggcgtgc cgattttgcg
    68161 ctccagttgt gcgacctttt catgcccgag gcaacgcatc gtcaataccc aaccaccata
    68221 gccacgctga atttggctgg cgataagatt aacccgcggc agccagggcg tatcgaaata
    68281 aaccgcttca accagcggct gcggagagcc ggtaatcaac cagatatcag catcggaact
    68341 taacaggtag gtggttaatc gctcctgaac cagcggaaag gcggtaacat tgtcacgaaa
    68401 ccagcgtacg aaatcggcct gcaacgcctg caaacgcgct tcattgtgac caaaagtgca
    68461 tccccacaga agcagactca tcggccagcg tgccgcacga ccttttatca ataacgcaat
    68521 ggctataatc ggcaacaacg gcaggacaag taacgcattc agcggttggc gacgcagtaa
    68581 atagcgcaga aaactgccga acatatcctg ctgatgcaat gttccatcta agtcaaaaaa
    68641 caccacacga cgctcgtgag ttgccaaacc atactcctct ggactgaaac atcctgatta
    68701 attttcgcta ctacatagcc taacagatag atcatcactt ttccggcaac aatcgaccgc
    68761 atcatggcta actggaattt ttaattcatg gcaattagcg gcaatggaat ataaaattca
    68821 ctcgcgtgtg tctcatattg cctgttgtcg ccactatttc atcgcgagga aagatatgaa
    68881 cggcttactt cgtatccgtc agcgttacca ggggtttgcc cagagtgata aaaaactggc
    68941 ggattatctg ctgctacaac ctgatacggc gcgccattta agctctcagc aactggccaa
    69001 cgaagccgga gtcagtcagt ccagcgtcgt gaagttcgcg caaaaactcg gctataaagg
    69061 ttttccggcg cttaagttgg cgttgagtga agcgctggca agccagccgg aatcgccctc
    69121 cgtgcccatt cataaccaaa tccgcggtga tgatccgtta cggctggtcg gcgaaaaact
    69181 gattaaagaa aatactggcg ctatgtacgc aacgctaaac gttaatagtg aagagaaact
    69241 gcatgaatgc gtaacaatgt tgcgctctgc gcggcggata attctgaccg gtattggcgc
    69301 ttcgggtctg gtggcgcaaa actttgcctg gaagctgatg aagattggct tcaatgctgc
    69361 cgcagtgcgc gatatgcatg cgctactcgc taccgtacag gcgtcgtccc ctgacgatct
    69421 gttattagcc atttcttaca ccggtgtacg acgcgagtta aacctggcgg cagatgagat
    69481 gctgcgagtg ggcggaaaag tgctggcgat taccggcttt actcccaatg ccctgcaaca
    69541 gcgtgcttct cattgcctgt ataccattgc cgaagaacaa gcgacaaaca gtgcttcaat
    69601 ctctgcttgt cacgcgcagg gaatgttaac ggatttgctg ttcattgcgc tgattcagca
    69661 ggatctggaa ctggcaccag aacgtattcg tcatagtgaa gcgctggtga aaaaactggt
    69721 ctgagtaaag aatgcgcgta taatgcccgc ccggtttgtg ttgttttgag agtttcctta
    69781 tggcgctgtt aatcactaaa aaatgcatca attgtgatat gtgtgaaccc gaatgcccga
    69841 atgaggcgat ttcaatggga gatcatatct atgagattaa cagcgataag tgtaccgaat
    69901 gcgtagggca ctacgagaca ccaacctgcc agaaggtgtg cccgatcccc aatactattg
    69961 tgaaagatcc gcaacatgtc gagacagaag aacagttgtg ggataaattt gtgctgatgc
    70021 accacgcgga taaaattt
//
LOCUS       DAPGOF010000002        62944 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.002, whole genome shotgun sequence.
ACCESSION   DAPGOF010000002 DAPGOF010000000
VERSION     DAPGOF010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 62944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62944
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.002"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            23..754
                     /locus_tag="RHV13_000059"
     CDS             23..754
                     /locus_tag="RHV13_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1239627.1"
                     /translation="MKKWKVRSALVALIVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     TVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIIHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVVDSYAANSAQGDMRNTGVIGFAAFELQGPAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            890..1810
                     /gene="speB"
                     /locus_tag="RHV13_000060"
     CDS             890..1810
                     /gene="speB"
                     /locus_tag="RHV13_000060"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HDV1239628.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(2129..2887)
                     /gene="loiP"
                     /locus_tag="RHV13_000061"
                     /gene_synonym="yggG"
     CDS             complement(2129..2887)
                     /gene="loiP"
                     /locus_tag="RHV13_000061"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HDV1239629.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQHGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            3165..5156
                     /gene="tkt"
                     /locus_tag="RHV13_000062"
     CDS             3165..5156
                     /gene="tkt"
                     /locus_tag="RHV13_000062"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDV1239630.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAEQLFEEFGFTVDNVVAKAKELL"
     gene            5470..5913
                     /gene="cmtB"
                     /locus_tag="RHV13_000063"
     CDS             5470..5913
                     /gene="cmtB"
                     /locus_tag="RHV13_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HDV1239631.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            5941..7329
                     /gene="cmtA"
                     /locus_tag="RHV13_000064"
     CDS             5941..7329
                     /gene="cmtA"
                     /locus_tag="RHV13_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HDV1239632.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGTVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIV
                     GGMSGTWMFNLLDGGLVAGSSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANVVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            7344..8621
                     /locus_tag="RHV13_000065"
     CDS             7344..8621
                     /locus_tag="RHV13_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDV1239633.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDTRLAQV
                     QKLLPVELAASKGIELVYVNTKGMSDPVQMLRALTGDAGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEKTHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            8618..9583
                     /gene="yggF"
                     /locus_tag="RHV13_000066"
     CDS             8618..9583
                     /gene="yggF"
                     /locus_tag="RHV13_000066"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HDV1239634.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNNIDGLAVTAMRQALN
                     EVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            9605..10114
                     /gene="fumE"
                     /locus_tag="RHV13_000067"
     CDS             9605..10114
                     /gene="fumE"
                     /locus_tag="RHV13_000067"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HDV1239635.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     EMPGFADDMTWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            10111..10824
                     /locus_tag="RHV13_000068"
     CDS             10111..10824
                     /locus_tag="RHV13_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HDV1239636.1"
                     /translation="MKIELTVNGLKVQAQYHDDEIENVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVMEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGLSLADAEAFYDRTDGPNVRRVLEES
                     LPAHLTLIMTATGEYRLVD"
     gene            complement(10796..11473)
                     /locus_tag="RHV13_000069"
     CDS             complement(10796..11473)
                     /locus_tag="RHV13_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HDV1239637.1"
                     /translation="MLTLNQITYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTTGTVILQQQAMKNLKNRQRAAKIGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTASAHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWMSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(11467..12144)
                     /locus_tag="RHV13_000070"
     CDS             complement(11467..12144)
                     /locus_tag="RHV13_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDV1239638.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEEEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWALERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(12132..12839)
                     /locus_tag="RHV13_000071"
     CDS             complement(12132..12839)
                     /locus_tag="RHV13_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDV1239639.1"
                     /translation="MHPFTSLTLWALAACTTLILPTQTILPVYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWTHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNNLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(12840..13418)
                     /locus_tag="RHV13_000072"
     CDS             complement(12840..13418)
                     /locus_tag="RHV13_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDV1239640.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKTLTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(13441..13872)
                     /locus_tag="RHV13_000073"
     CDS             complement(13441..13872)
                     /locus_tag="RHV13_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDV1239641.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQQQLHAAWIA
                     GCTGSLTDIALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            14244..15263
                     /gene="epd"
                     /locus_tag="RHV13_000074"
     CDS             14244..15263
                     /gene="epd"
                     /locus_tag="RHV13_000074"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HDV1239642.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            15313..16476
                     /gene="pgk"
                     /locus_tag="RHV13_000075"
     CDS             15313..16476
                     /gene="pgk"
                     /locus_tag="RHV13_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDV1239643.1"
                     /translation="MSVNKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            16691..17770
                     /gene="fbaA"
                     /locus_tag="RHV13_000076"
     CDS             16691..17770
                     /gene="fbaA"
                     /locus_tag="RHV13_000076"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HDV1239644.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            18129..18989
                     /gene="mscS"
                     /locus_tag="RHV13_000077"
     CDS             18129..18989
                     /gene="mscS"
                     /locus_tag="RHV13_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HDV1239645.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            19128..19763
                     /gene="argO"
                     /locus_tag="RHV13_000078"
     CDS             19128..19763
                     /gene="argO"
                     /locus_tag="RHV13_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HDV1239646.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            19856..20596
                     /locus_tag="RHV13_000079"
     CDS             19856..20596
                     /locus_tag="RHV13_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HDV1239647.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            20764..21660
                     /locus_tag="RHV13_000080"
     CDS             20764..21660
                     /locus_tag="RHV13_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV1239648.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTSH"
     gene            complement(21657..23135)
                     /gene="scpC"
                     /locus_tag="RHV13_000081"
     CDS             complement(21657..23135)
                     /gene="scpC"
                     /locus_tag="RHV13_000081"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HDV1239649.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(23159..23944)
                     /gene="scpB"
                     /locus_tag="RHV13_000082"
     CDS             complement(23159..23944)
                     /gene="scpB"
                     /locus_tag="RHV13_000082"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HDV1239650.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(23955..24950)
                     /gene="meaB"
                     /locus_tag="RHV13_000083"
     CDS             complement(23955..24950)
                     /gene="meaB"
                     /locus_tag="RHV13_000083"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HDV1239651.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNALRLGVTGTPGAGKSTFLEAFGMLLIRDGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAETAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(24943..27087)
                     /gene="scpA"
                     /locus_tag="RHV13_000084"
     CDS             complement(24943..27087)
                     /gene="scpA"
                     /locus_tag="RHV13_000084"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HDV1239652.1"
                     /translation="MSNVQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEAAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(27291..28184)
                     /gene="argP"
                     /locus_tag="RHV13_000085"
     CDS             complement(27291..28184)
                     /gene="argP"
                     /locus_tag="RHV13_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HDV1239653.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            28326..28556
                     /locus_tag="RHV13_000086"
     CDS             28326..28556
                     /locus_tag="RHV13_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1239654.1"
                     /translation="MHFAQRVRALVVLNGVALLPQFACKQGLANGELVRLFAPWSGIP
                     RLLHALFAGRKGMPAIARYFMDELTTRLANGV"
     gene            28612..29271
                     /gene="rpiA"
                     /locus_tag="RHV13_000087"
     CDS             28612..29271
                     /gene="rpiA"
                     /locus_tag="RHV13_000087"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HDV1239655.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            29527..30759
                     /gene="serA"
                     /locus_tag="RHV13_000088"
     CDS             29527..30759
                     /gene="serA"
                     /locus_tag="RHV13_000088"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDV1239656.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            30988..31047
                     /gene="ibsC"
                     /locus_tag="RHV13_000089"
     CDS             30988..31047
                     /gene="ibsC"
                     /locus_tag="RHV13_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HDV1239657.1"
                     /translation="MMRLVIILIVLLLISFPAY"
     gene            31380..31439
                     /gene="ibsC"
                     /locus_tag="RHV13_000090"
     CDS             31380..31439
                     /gene="ibsC"
                     /locus_tag="RHV13_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HDV1239658.1"
                     /translation="MMRLVIILIVLLLISFSAY"
     gene            complement(31540..32142)
                     /locus_tag="RHV13_000091"
     CDS             complement(31540..32142)
                     /locus_tag="RHV13_000091"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDV1239659.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRTLTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKMQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(32163..32346)
                     /gene="ssrS"
                     /locus_tag="RHV13_000092"
     ncRNA           complement(32163..32346)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="RHV13_000092"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(32388..32717)
                     /gene="zapA"
                     /locus_tag="RHV13_000093"
     CDS             complement(32388..32717)
                     /gene="zapA"
                     /locus_tag="RHV13_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDV1239660.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTREYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            32885..33463
                     /gene="ygfB"
                     /locus_tag="RHV13_000094"
     CDS             32885..33463
                     /gene="ygfB"
                     /locus_tag="RHV13_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HDV1239661.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            33489..34814
                     /gene="pepP"
                     /locus_tag="RHV13_000095"
     CDS             33489..34814
                     /gene="pepP"
                     /locus_tag="RHV13_000095"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HDV1239662.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPIVHEMRLFKSPEEIAVLRRAGEITALAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSIQEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     AEVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            34811..35989
                     /gene="ubiH"
                     /locus_tag="RHV13_000096"
                     /gene_synonym="visB"
     CDS             34811..35989
                     /gene="ubiH"
                     /locus_tag="RHV13_000096"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HDV1239663.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGVLPVHLIEAIAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLECGETLTGRVLVA
                     ADGTHSTLATVCGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTHAARS
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            36012..37214
                     /gene="ubiI"
                     /locus_tag="RHV13_000097"
                     /gene_synonym="visC"
     CDS             36012..37214
                     /gene="ubiI"
                     /locus_tag="RHV13_000097"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HDV1239664.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAHQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQAGEGEFNRALNIAFDNRLGLCKVESERQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(37288..37371)
                     /locus_tag="RHV13_000098"
     CDS             complement(37288..37371)
                     /locus_tag="RHV13_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1239665.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            37661..38755
                     /gene="gcvT"
                     /locus_tag="RHV13_000099"
     CDS             37661..38755
                     /gene="gcvT"
                     /locus_tag="RHV13_000099"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HDV1239666.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            38779..39168
                     /gene="gcvH"
                     /locus_tag="RHV13_000100"
     CDS             38779..39168
                     /gene="gcvH"
                     /locus_tag="RHV13_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HDV1239667.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            39287..42160
                     /gene="gcvP"
                     /locus_tag="RHV13_000101"
     CDS             39287..42160
                     /gene="gcvP"
                     /locus_tag="RHV13_000101"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HDV1239668.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATAPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPIGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLADLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(42326..43759)
                     /gene="bglA"
                     /locus_tag="RHV13_000102"
     CDS             complement(42326..43759)
                     /gene="bglA"
                     /locus_tag="RHV13_000102"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HDV1239669.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDET
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDILREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQKPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGENL"
     gene            43804..44115
                     /locus_tag="RHV13_000103"
     CDS             43804..44115
                     /locus_tag="RHV13_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDV1239670.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELKKVIADIYPGQTQFYVIEFKC
                     L"
     gene            44279..44938
                     /locus_tag="RHV13_000104"
     CDS             44279..44938
                     /locus_tag="RHV13_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDV1239671.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(45015..45995)
                     /gene="ygfZ"
                     /locus_tag="RHV13_000105"
     CDS             complement(45015..45995)
                     /gene="ygfZ"
                     /locus_tag="RHV13_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HDV1239672.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVRESQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVREGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLTGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            46238..46504
                     /gene="sdhE"
                     /locus_tag="RHV13_000106"
     CDS             46238..46504
                     /gene="sdhE"
                     /locus_tag="RHV13_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HDV1239673.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            46485..46892
                     /locus_tag="RHV13_000107"
     CDS             46485..46892
                     /locus_tag="RHV13_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HDV1239674.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     SGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(46932..47453)
                     /gene="fldB"
                     /locus_tag="RHV13_000108"
     CDS             complement(46932..47453)
                     /gene="fldB"
                     /locus_tag="RHV13_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HDV1239675.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQGW
                     CEQILNEMAEHYA"
     gene            47565..48461
                     /gene="xerD"
                     /locus_tag="RHV13_000109"
     CDS             47565..48461
                     /gene="xerD"
                     /locus_tag="RHV13_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDV1239676.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            48486..49196
                     /gene="dsbC"
                     /locus_tag="RHV13_000110"
     CDS             48486..49196
                     /gene="dsbC"
                     /locus_tag="RHV13_000110"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HDV1239677.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            49202..50935
                     /gene="recJ"
                     /locus_tag="RHV13_000111"
     CDS             49202..50935
                     /gene="recJ"
                     /locus_tag="RHV13_000111"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDV1239678.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERNIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            51026..52124
                     /gene="prfB"
                     /locus_tag="RHV13_000112"
     CDS             join(51026..51100,51102..52124)
                     /gene="prfB"
                     /locus_tag="RHV13_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDV1239679.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            52134..53651
                     /gene="lysS"
                     /locus_tag="RHV13_000113"
     CDS             52134..53651
                     /gene="lysS"
                     /locus_tag="RHV13_000113"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDV1239680.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFGGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(53694..54242)
                     /gene="idi"
                     /locus_tag="RHV13_000114"
     CDS             complement(53694..54242)
                     /gene="idi"
                     /locus_tag="RHV13_000114"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HDV1239681.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            54297..54368
                     /locus_tag="RHV13_000115"
     CDS             54297..54368
                     /locus_tag="RHV13_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1239682.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(54365..54490)
                     /locus_tag="RHV13_000116"
     CDS             complement(54365..54490)
                     /locus_tag="RHV13_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1239683.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(54492..55940)
                     /gene="uacT"
                     /locus_tag="RHV13_000117"
     CDS             complement(54492..55940)
                     /gene="uacT"
                     /locus_tag="RHV13_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HDV1239684.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            56376..58295
                     /gene="ygfT"
                     /locus_tag="RHV13_000118"
     CDS             56376..58295
                     /gene="ygfT"
                     /locus_tag="RHV13_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HDV1239685.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGLAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            58295..58783
                     /locus_tag="RHV13_000119"
     CDS             58295..58783
                     /locus_tag="RHV13_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDV1239686.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPLGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(58819..60186)
                     /gene="ghxQ"
                     /locus_tag="RHV13_000120"
                     /gene_synonym="ygfQ"
     CDS             complement(58819..60186)
                     /gene="ghxQ"
                     /locus_tag="RHV13_000120"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HDV1239687.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(60222..61538)
                     /gene="guaD"
                     /locus_tag="RHV13_000121"
     CDS             complement(60222..61538)
                     /gene="guaD"
                     /locus_tag="RHV13_000121"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HDV1239688.1"
                     /translation="MSGKHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWENGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(61556..>62944)
                     /gene="xanQ"
                     /locus_tag="RHV13_000122"
     CDS             complement(61556..>62944)
                     /gene="xanQ"
                     /locus_tag="RHV13_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HDV1239689.1"
                     /translation="NHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPALIVGA
                     ALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIALGSS
                     MKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGLSLIK
                     VGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGLCVGY
                     IASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGDITAT
                     AMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGVASRY
                     VGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGLKRRE
                     TLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGYRQEN
                     VLPGITSAEEMD"
BASE COUNT        15256 a        15801 c        16808 g        15079 t
ORIGIN      
        1 attaaatgca gagggaacgg ttatgaaaaa atggaaagta cgtagcgcgt tggtggcatt
       61 aatcgtgctg ctggccggtt gttccagtaa tgcgcagtat aatagcagtg catcgggcaa
      121 tgtcggcacc gcctggggtg gggatgtgca ctcaacggtt cagggcgtat ctgctgagcg
      181 agcctggcgc gatccggcag agatgatcgt tattagttat tcgactaatg ttccttccgg
      241 ctacgaccgc gtttatagca ttcgtattaa cgaactggaa tatgctattc gcgatggtaa
      301 ttttaattct ctacctatta cgcgggttta tgattcatcc aataatgaac cacgctatat
      361 tatccatgcc agagtaggaa tgaattatca attatatgtc cgcaattaca gtcgtaacac
      421 caactacgag atcgttgcta cggttgatgg cctggatgtg ctgaacggca agcaaggttc
      481 gctgaataac aacggctata ttgtgaatgc cggagattcg ttagcgatta aaggattccg
      541 caaagataaa cataccgaag cggcttttca gttcgccaac gttgttgatt cctacgcagc
      601 caattcggcg caaggcgata tgcgtaatac cggggtaatc ggctttgccg cctttgaatt
      661 acaaggcccg gcgcaaaacg cgctgccacc gtgctctgga caagcttttc cagcagataa
      721 caatggctat gcgccgccac cgtgtcggaa atagcaacct cgcttgatgc ctcgcccatt
      781 taaggcgata attagcacaa acttgtgttt cgcaaatcca acccttcctc gtcgggccta
      841 acgacgcgga agggtttttt tatatcgact ttgtaatagg agtccatcca tgagcacctt
      901 aggtcatcaa tacgataact cactggtttc caacgccttt ggttttttac gcctgccgat
      961 gaacttccag ccgtatgaca gcgatgccga ctgggtgatt actggtgtgc cgttcgatat
     1021 ggccacttct ggtcgtgcgg ggggacgcca cggcccggca gctatccgtc aggtttcgac
     1081 taacctggcc tgggaacaca accgcttccc gtggaatttc gacatgcgcg agcgtctgaa
     1141 cgtggtggac tgcggcgatc tggtatatgc cttcggcgat gcccgtgaga tgagcgaaaa
     1201 actgcaggca cacgccgaga agctgctggc tgccggtaag cgtatgctct ccttcggtgg
     1261 tgaccacttt gttacgctgc cgctgctgcg tgctcatgcg aagcatttcg gcaaaatggc
     1321 gctggtacac tttgacgccc acaccgatac ctacgcgaac ggttgtgaat ttgaccacgg
     1381 caccatgttc tataccgcgc cgaaagaagg tctgatcgac ccgaatcatt ccgtgcagat
     1441 tggtattcgt accgaatttg ataaagacaa tggctttacc gtgctggacg cctgccaggt
     1501 gaacgatcgc agcgtggatg acgttatcgc ccaggtgaaa cagattgtgg gtgatatgcc
     1561 ggtttacctg acctttgata ttgactgcct ggatcctgct tttgcaccag gcaccggtac
     1621 cccggtaatt ggcggcctga cctccgatcg cgctattaaa ctggtacgcg gcctgaaaga
     1681 tctcaacatc gttgggatgg acgtagtgga agtggctccg gcatacgatc agtcggaaat
     1741 cactgctctg gcagcggcaa cgctggcgct ggaaatgctg tatattcagg cggcgaaaaa
     1801 gggcgagtaa gcaccagatg cgatgcgcac gggtaaaacg tgccattaat gtcggatgcg
     1861 gcgtgaacgc cttatccgac ctacgttcgg cacccgtagg ccggataaga tgcgccagca
     1921 tcgcatccgg caatgcgcac aaggtaacaa atgtgccatt catgtcagat gcggcgtgaa
     1981 cgccttatct gacctacgtt tggcacccgt aggccggata agatgcgcca gcatcgcatc
     2041 cggcaatgcg cacgaggtag tgaatgtgcc atttatgtca gatgcggcgt aaatgcctta
     2101 tctgaactac gtttggcacc accatgcctt acttaatccc gtccgcgctc atacgatcgc
     2161 gaatatgctg ggcgcgttcg gcggatgcag gatggtcgtc aaacattgag ctttggcgac
     2221 cttcttccag ttttgccagt ttttcaaagc tggtggcaag acctgccgga ctgatgccgt
     2281 gttggcgcag taaatcgtag gagtaatcat ccgcctctgc ttcctggcgc tgggagaatt
     2341 gcgaattgac taatttctcg cccagatcac cgagttgtga ttgagataaa ctgccgacga
     2401 taccgcccgc agaggccgcc gcgactcgca cggcatttgt accaagtgcc acctgcatcc
     2461 ctttcttcac atggcctaac gccacgtgcc ccatttcgtg accgatcacc gcttcgactt
     2521 cgttatccgt catcatatcc atcagcccgc tatagacgcg gatacagccg ttagccattg
     2581 caaaggcgtt cacatccttc gccatataca ctttgtaatt taccggctga ccgttgatat
     2641 tgttgcctag cgcattggca atagttgtca gacgtttagc gtattcgcta ttggctggcg
     2701 caatcgtcgc cttgctgtcc atctcctgac atgcctgatc gctcagggtt ttcacctgcg
     2761 catcactcaa actgtaagcc tgaaaagctt ccgctcctga tgagagcagt ccgttggagt
     2821 ccatattctg gcaaccagtc agtaccgttg ccacgctcat tgctaccaat aaggcgcgaa
     2881 ttttcatgtt ttttcttccg cacaaggtca ggattattct aaaaatgcgc cgtttgcagg
     2941 tgaaccgacg ctcagtctca gtataaggaa aagcgcagcg gacgggcgag tagattgcgc
     3001 aacatgcgag catgatccag agatttctga agcggcaaaa ggatgttcca tgtacatgac
     3061 gcgcggcttg cggtaaattg ttggcaaatt ttccggcgta gcccaaaacg cgctttcgtc
     3121 aagtcgttaa gggcgtgccc ttcatcatcc gatctggagt caaaatgtcc tcacgtaaag
     3181 agcttgccaa tgctattcgt gcgctgagca tggacgcagt acagaaagcc aaatccggtc
     3241 acccgggtgc ccctatgggt atggctgaca ttgccgaagt cctgtggcgt gatttcctga
     3301 aacacaaccc gcagaatccg tcctgggctg accgtgaccg cttcgtgctg tccaacggcc
     3361 acggctccat gctgatctac agcctgctgc acctcaccgg ttacgatctg ccgatggaag
     3421 aactgaaaaa cttccgtcag ctgcactcta aaactccggg ccacccggaa gtgggttaca
     3481 ccgctggtgt ggaaaccacc accggtccgc tggggcaggg tattgccaac gcagtcggta
     3541 tggcgattgc agaaaaaacg ctggcggcgc agtttaaccg tccgggccac gacattgtcg
     3601 accactacac ctacgccttc atgggcgacg gctgcatgat ggaaggcatc tcccacgaag
     3661 tttgctctct ggcgggtacg ctgaagctgg gtaaactgat tgcattctac gatgacaacg
     3721 gtatttctat cgatggtcac gttgaaggct ggttcaccga cgacaccgca atgcgtttcg
     3781 aagcttacgg ctggcacgtt attcgcgaca tcgacggcca tgacgcagca tccatcaaac
     3841 gcgcagttga agaagcgcgc gcagtgactg acaaaccgtc cctgctgatg tgcaaaacca
     3901 tcatcggttt cggttccccg aacaaagccg gtacccacga ctcccacggt gcgccgctgg
     3961 gcgacgctga aattgccctg acccgcgaac agctgggctg gaaatacgcg ccgttcgaaa
     4021 tcccgtctga aatctatgcg cagtgggatg cgaaagaagc aggtcaggcg aaagaatctg
     4081 catggaacga gaagtttgct gcttacgcca aagcttatcc gcaggaagct gctgaattca
     4141 cccgtcgtat gaaaggcgaa atgccgtctg acttcgacgc gaaagcgaaa gagttcattg
     4201 ctaaactgca ggctaatccg gcgaaaatcg ccagccgtaa agcgtctcag aatgctattg
     4261 aagcgttcgg cccgttgttg ccggaattcc tcggcggctc tgctgacctg gcaccgtcta
     4321 acctgaccct gtggtctggt tctaaagcaa tcaacgaaga tgctgcgggt aactacatcc
     4381 actacggtgt tcgcgagttc ggtatgaccg cgattgctaa cggtatctcc ctgcacggtg
     4441 gtttcctgcc gtacacctcc accttcctga tgttcgtgga atacgcacgt aacgccgtac
     4501 gtatggctgc gctgatgaaa cagcgtcagg tgatggttta cacccacgac tccatcggtc
     4561 tgggcgaaga cggcccgact caccagccgg ttgagcaggt cgcttctctg cgcgtgaccc
     4621 cgaacatgtc tacatggcgt ccgtgtgacc aggttgaatc cgcggtcgcg tggaaatacg
     4681 gtgttgagcg tcaggacggt ccgaccgcgc tgatcctctc ccgtcagaac ctggcgcagc
     4741 aggagcgtac tgaagagcaa ctggcaaaca tcgcgcgcgg tggttatgtg ctgaaagact
     4801 gtgctggtca gccggaactg atcttcatcg ccaccggttc tgaagttgaa ctggcggttg
     4861 ctgcctacga aaaactgact gccgaaggcg tgaaagcgcg cgtggtttcc atgccgtcta
     4921 ccgacgcatt cgacaagcag gacgcggctt accgtgagtc tgtcctgccg aaagcggtta
     4981 ctgcacgcgt tgcggtggaa gcgggtattg ctgactactg gtacaagtat gttggcctga
     5041 acggtgctat cgtcggtatg accaccttcg gtgaatctgc tccggcagag caactctttg
     5101 aagagttcgg cttcaccgtt gacaacgttg ttgcgaaagc aaaagaactg ctgtaattag
     5161 cattttgggt aaaaaggtcg cttcggcgac cttttttatt accttgacat gtccgttttc
     5221 ggacgagcaa tagataaagc gtgttgtaga tcacaaatat ttatatgcaa taaatatcaa
     5281 ttatgtaata tgcatcacga tatgcgtgtt gacatttgtt gttatatcta taactcaatg
     5341 ttatataaga aattaataat tcactgtttt caaaacaccg gtttccctgc tcaattgctt
     5401 tcattaaacc gcacgagtat tttattttta ccgccggacg gtaccctaaa aataaaataa
     5461 ggataattta tgcggcttag tgattatttt ccagaatcat caatctcagt tatacattca
     5521 gcaaaagatt ggcaggaagc tatcgatttc tcgatggtat cattgctgga taaaaactat
     5581 atcagcgaga attacattca ggcaattaaa gattccacca ttaacaatgg cccttattat
     5641 attctcgcac caggcgtggc aatgcctcat gcgcgaccgg aatgtggggc gcttaaaacc
     5701 gggatgtctt tgacattact tgaacaaggt gtttattttc cggggaatga cgaaccaatt
     5761 aaattactca tcggactctc tgctgccgat gccgattcgc acattggcgc tattcaggcg
     5821 ttaagtgaat tactgtgcga agaagaaata ctcgaacaac tcttaacagc atcatcagaa
     5881 aaacaattag cggacattat cagccgcgga taattactct ctcctctaca ggataatatt
     5941 atggaaaaca agtctgctcg tgcaaaggtc caggcttttg ggggcttttt gactgcaatg
     6001 gtcatcccca atattggtgc ttttattgcc tggggtttta ttactgcgtt atttattccc
     6061 accggttggc tgcctaacga acatttcgcc aaaattgtcg gcccgatgat tacctattta
     6121 ttgcccgtga tgattggttc taccggtggt catctggtcg gcggtaaacg cggcacggtc
     6181 atgggcggaa taggtactat tggtgtgatc gtcggcgcag agatcccgat gttccttggc
     6241 tcaatgatta tggggccgct cggtgggctg gtcataaaat atgtcgataa ggcactggaa
     6301 aaacgcatac ctgctggttt tgagatggtt atcaataact tctcattagg tatcgcgggg
     6361 atgctccttt gcctgctggg ttttgaagtg atcggcccgg cggtgttaat tgccaatact
     6421 ttcgtcaaag agtgtattga ggcgctggta catgcgggtt atctgcctct gctgtcagtc
     6481 atcaatgaac cggcgaaagt gcttttcctt aataatgcga tcgatcaggg cgtctattat
     6541 ccgctgggaa tgcaacaggc ttcggttaac ggtaaatcca tcttctttat ggtggcctct
     6601 aacccaggtc cgggcctggg gctgctgctg gcgtttacct tgtttggtaa agggatgagt
     6661 aaacgttctg cgcccggggc gatgattatt cacttcctcg gtgggatcca cgaactgtat
     6721 ttcccatatg tgctgatgaa gccgctgact attattgcca tgattgtggg cggtatgtct
     6781 ggcacctgga tgtttaactt actggacggt ggtctggtgg ctggctcaag tccggggtct
     6841 atctttgctt acctggcgct gacgccgaaa ggttcgttcc tggcgacaat tgccggtgtt
     6901 acggtaggta ccctggtgtc ctttgctatc acttcgctga tactgaagat ggaaaaaacg
     6961 gtggaaacgg agagcgaaga tgagtttgct cagtcagcca atgtggttaa ggcgatgaaa
     7021 caagagggtg cattctcgtt aagcagggtt aagcgtatcg cctttgtttg cgatgcgggg
     7081 atgggctcca gtgcgatggg cgcgaccacc ttccgtaaac gcctggaaaa agcggggctg
     7141 gcaattgaag taaaacatta cgccatagaa aacgtgcctg cggatgcgga tatcgtcgtt
     7201 actcatgcca gtctggaagg gcgcgtgaaa cgtgtgacgg ataaaccact gatattgatt
     7261 aataactata ttggcgatcc aaaactcgac actttattta atcaattaac cgccgaacat
     7321 aaacactgat tacagaggta aaaatgaaaa ccaaagttgc tgctatttat ggcaagcggg
     7381 atgtccgtct gcgcgaattt gaactaccag aaattaccga taatgaatta ctggtgagtg
     7441 tgatttctga cagcgtctgt ttatccacct ggaaagcggc gttactcggt agtgaacata
     7501 aacgcgtacc cgacgattta gaaaatcatc cggtcattac cgggcatgaa tgtgccgggg
     7561 ttattgtcga agtgggtaaa aatctcactg gcaaatataa aaaaggccag cgttttgtat
     7621 tgcaaccggc gatgggatta ccaagcggat attcagcggg ctacagctac gaatattttg
     7681 gcggcaatgc cacttatatg attattcccg aaatcgccat taatttgggc tgcgtattac
     7741 cgtatcacgg ctcttatttt gctgcggcgt cgctggcaga gcctatgtgc tgcattattg
     7801 gtgcttatca tgccaattat cacaccacgc aatatgttta tgagcatcgc atgggcgtca
     7861 aacctggcgg caatattgcg ctactggcgt gtgcaggtcc gatgggcatt ggcgctatag
     7921 attacgccat taacggcggc atacaaccgt cgcgggtggt ggtggtcgat atcgacgaca
     7981 cacgtctggc gcaggtacag aagctgctgc cggtggaact ggcggccagt aaaggcattg
     8041 agctggtgta tgtgaatacc aaagggatga gcgatcctgt ccagatgctg cgggcgctga
     8101 caggagatgc cgggttcgat gacatttttg tttatgcggc ggtgcctgct gtcgttgaga
     8161 tggctgatga attactggcg gaagatggct gtctgaactt ctttgccggg ccgacggata
     8221 aaaacttcaa agtgccgttt aatttctaca acgttcatta caacagcacg cacgtcgtcg
     8281 gtacatccgg cggttcaacg gacgacatga aagaggcgat tgcccttagc gccactgggc
     8341 agttacagcc gtcgtttatg gtgacccata tcggtggcct ggatgcggtg ccagaaaccg
     8401 tgctcaatct gccggatatc cctggcggta aaaaactcat ttataacggc gtgaccatgc
     8461 cgctcactgc cattgccgat tttgccgaaa aaggcaaaac cgatccgctg tttaaagagt
     8521 tggcgcggct ggttgagaaa acgcacggca tctggaatga acaggccgag aaatatctgc
     8581 tggcacaatt tggcgttgat atcggggagg ccgcgcaatg atgtccctgg cgtggccatt
     8641 atttcgcgtt acggaacagg cagcgttggc tgcctggccg caaaccggat gtggcgacaa
     8701 aaataacatt gatggcctgg cggtcaccgc gatgcgccag gcattaaacg aagtcgcttt
     8761 tcgtggacga gtggttatcg gcgaaggaga aattgaccat gcgccgatgc tgtggattgg
     8821 agaagaggtg ggcaaaggcg atgggccaga agtcgatatc gcggttgatc ccattgaagg
     8881 cacgcggatg gtggcgatgg ggcagagcaa tgcgctggcg gtcatggctt tcgcccctcg
     8941 agatagcctg ttacatgcgc ccgatatgta tatgaaaaag ctggttgtta atcgactggc
     9001 tgccggggcg attgatctgt ctttgcctct ggcggacaac ttgcgtaacg tggcgcgggc
     9061 gttaggtaaa ccgctggaca agctgcgcat ggttacgctg gataaaccgc gtctgagtgc
     9121 cgcgattgaa gaagcaacac agctgggcgt gaaggttttt gccctgccgg atggcgatgt
     9181 cgccgccagc gtgctgacct gctggcagga taatccttac gatgtgatgt acaccatcgg
     9241 cggtgcgccg gaaggcgtga tttctgcctg tgccgttaaa gcattaggcg gggatatgca
     9301 ggccgagctg attgactttt gtcaggctaa aggggattac acggaaaatc ggcagattgc
     9361 agagcaggag cgcaagcgtt gcaaggcaat gggcgttgac gttaaccgtg tgtactcgct
     9421 cgatgaactg gtgaggggga acgatatcct ctttagcgct acgggcgtga cgggcggtga
     9481 gctggtgaac ggtatccaac agacggcgaa tggggtgcgg acgcagacat tactgatcgg
     9541 cggcgcggac caaacgtgta atataataga ctccctgcat tgatggtgat ctatcggaag
     9601 accaatggcg acgctgacag aagatgatgt gcttgagcaa ctggatgcac aggacaattt
     9661 attttcattt atgaaaacgg cgcattctat tttgctccag gggatacgcc agtttctacc
     9721 gtcgctgttt gtcgataacg atgaagagat cgtcgaatat gcagtgaagc cgttactcgc
     9781 ccaaagcggc ccgcttgacg atattgatgt tgcgctgcgt ttgatttatg cgctggggaa
     9841 aatggataaa tggctgtacg ccgatatcac gcacttttcc cagttctggc attacctgaa
     9901 cgaacaggat gaaatgcccg gatttgccga tgacatgacc tgggatttta tcagcaacgt
     9961 caatagcata acccgcaatg cgacgctcta tgacgcgttg aaagcgatga agttcgccga
    10021 tttcgccgtc tggtcagagg cgcgttttag cgggatggtc aaaacggcgc tgacgctggc
    10081 agtaacgaca accttaaagg aattaacgcc gtgaaaattg aattaacggt gaatggtctg
    10141 aaggtacagg cacagtacca tgatgatgag atagaaaatg tacataaacc gttgctgcgc
    10201 atgttggcgg ccttacagac ggtaaatccg cagcggcgta cggtagtttt tctttgcgct
    10261 ccgccgggga cggggaaatc taccctaacc accttctggg aatatctcgc acaacaagac
    10321 ccggaactac ccgctattca gacgctcccg atggatggtt ttcaccatta caatagctgg
    10381 ctggatgcgc atcaattgcg ccccttcaaa ggcgcaccag agacatttga cgttgcgaaa
    10441 ctggcggaaa atctgcgcca ggtaatggaa ggggattgta cgtggccaca gtacgatcga
    10501 caaaagcatg atcctgttga agatgcgttg cacgttaccg caccactcgt catcgtcgaa
    10561 ggaaactggt tgttgctgga cgacgagaag tggtgccagc ttgcgcaatt ttgtgatttc
    10621 tcgatcttta tcaacgcgcc agctactgcg ttgcgggaac gactggtggg gcgcaagctg
    10681 gcgggagggt tatcgctggc agacgcagaa gccttttatg accgtaccga tgggccgaat
    10741 gttcgccggg tgctggaaga gagtctgcct gcacatttga ccttaataat gaccgctacg
    10801 ggtgaatatc gtctggtgga ttaacgttcc tgattacacc atcttccagt cgcaccaccc
    10861 gagagaaatg acgccgggta aatgcggcgt catgactgat tgctacgacg ctcgtgcctc
    10921 gctggcggca cttttccagc cagctttcaa acacgctcat ccaattttcg tcaaaatccc
    10981 gactcggctc gtccagcagc aatagcggcg gagagagtgc ttccaggcag gcgacggcga
    11041 ccatccgtcg ctgcgcacta tgcagatcta acggatgcgc gctggcagtg tctgctaact
    11101 gacagcattg cagggcagca tgggtacgtt gagtgatttc atccgccggg catttttgca
    11161 gtttcaaacc aaatgcgatc tcgtcggcga cggtgctgtg aaaaagctgg ttttccgctt
    11221 cctgaaacag tacgccaatc tttgctgcac gttggcggtt tttcaggttt ttcatcgcct
    11281 gttgttgcaa tataaccgta ccggttgtgg gcgtaagaag acctgccatt actcgtaaca
    11341 gcgtcgattt tcccgcgcca ttgtcgccgg tcagcgccaa ccattcacct tgtttgagtt
    11401 gcagagaaat gtcgcaaaga caatccgttg ccgcgccagg ccagcgataa gttatctgat
    11461 ttaacgttag catagtggct ggagcgctcc gttttgcaat tgccaggctt gctggcagta
    11521 gttcaggaaa ggtgtgcgat gacgttcaaa aagaataatc agcgaaccac gttcaagcgc
    11581 ccagtgttgt aagcgctgca acagcatttc gctggcctgc ggcgtcaggc gactaaaggc
    11641 ttcatcaagt attaagagct ttggctgcat cgcgatggcg caggcgatga ccacgcgctg
    11701 tgtctctcca ccggagagtg ttgccgggtg acgatggcgc aacggctggc actcggtcag
    11761 agtcagggcg gcatcaatcc gcgccataat ctcttcttca gcaagacaca gattttctgg
    11821 cccaaaggca acttcttctt ccacgctaaa ggtacagccg gagagctgca aatagggcga
    11881 ttgctgaacg agctggatgg tggcggattg ttcattaagc gggagatgcc caatgggcgt
    11941 accgagaagc gtccccgtgc cggttatctc tcctggcaga aagtccggat accagcccgc
    12001 catcagctgt gccagcgtac ttttgccact gccgttgtca ccaaagatgg cgaccatccc
    12061 cggtttgaca tagtgaaagt cataacagaa cggaggatgc gttgagtgtg tcgggcaata
    12121 acggaactgt tctaacgtaa ccatatccac gctccgaatt ctgtcagcat cagcaagatc
    12181 atggtgtaac gcgctacgcg ttgtagggtg ctatcggctg gtgcccacaa cgttgttcgt
    12241 cggttgtgga tccgaaatgc ccgcatatcc agcgccgcac cacggacagc aagattgttt
    12301 agcgcattgt gtgttagcgg aataattaac gcgggcatcg cacgtaaacg ctgataccag
    12361 ccctcatcca gcggcacgcc gcgggcacgc tgcgcttcat gaataatcgc taattgccgt
    12421 tttaactgct caacgaccaa taacggcccg gcaaacagat aagcgacgcc tggcggcagg
    12481 cgagaagcaa acagagcgcg gataaaacat tgcacgggga cgtactgcat ccacaactgt
    12541 gaggttgaaa caatcgccag aatccgcaac cagagcgtaa tagcatgcgt ccaacgctcc
    12601 ggcgaacgcg gcgtgccgct tagccattct gttagccagc caccgtgtac cagccacagc
    12661 ccggctccca gcgagaacat cagccagacg acatattttg cccgccgccg cgtggccttt
    12721 agcgcaataa ggcagaaaaa ggtcgccgcg ctatagactg gcagtattgt ctgtgtgggc
    12781 aggataagcg tggtgcaggc cgccaatgcc catagtgtta atgaggtaaa tggatgcatt
    12841 tagcgcacgg cagccattgc cggaaaatgg cgcgtggtgc gaatcggcag ctggcgcagt
    12901 aaaagccaga caatcaccgc tgtaagagtt ttatcgacca ggttcgcgcc tatcaccgta
    12961 atcgccaccg actccaccag gttttgcccc atcgagtgca tccaggcaac gaacaaatcg
    13021 gctccgctac cggtgacacc gccgaacagc gcagtacgca gcggtacagc gacgaccgtt
    13081 accgcaagcg taataatcac gccgctgaca acgacttttg gcagggtgcg aaaccaaccc
    13141 gctcgcgcca gccaaccggc gaccaggccg atgaccatcg cgacgggtgc aaatgcggcg
    13201 gcgatcgggt cggtcagcaa tccccagagt aaattggtca gtaagccggt cagcattccg
    13261 atcaccggac caagcagtac cgcgctaata agtgtgccga tggagtcgag aaatataggt
    13321 aatttgacca tgctggccag ttgcccgccg atcatattga tggcgataga tataacgatc
    13381 aacaccagtg cctgactgga aaagtggcta cgcgccataa gaaaacctct ctgaaagtag
    13441 ttatttaacg ggggaaatat gcaactcgtc gtaaccagaa agcgcgcaag gttggcggct
    13501 aaacgccagt tcctcaaggc taccgatcac cagttcaagc gtggtccgca cggtacagcc
    13561 gggcatcata tgaccgccaa gcatcgtgcc gtgtggatcg gaaacgcaga gatgaagatg
    13621 ctcaccactc tgttccaacg tgccgtttag tgagatcact tcgaatgtac cgttcaggag
    13681 cgtggtgcct tcttgcccgg catagcgcaa ggcgatgtca gtcaggctac cggtacagcc
    13741 tgcaatccag gcggcatgaa gttgctgctg ctgtgcaaaa gcgtgtagct gggaaaaaac
    13801 ttcctgcccg gggaggaggc gcagtgcgta aaagcgtgcg gaggaagaat atgggtaggg
    13861 caatgtcatc attggagtac tccgaaactc ggacgtattg cggataatat cgacgctgca
    13921 tcagtcatat tctgatataa gtcagggttt gtgacggcac aacgaaaaga ttttgctacg
    13981 cttattcgtg attctgcaaa gtggaataaa ttccacaggg gcggggctga aaacgtgacg
    14041 taagtcagat atttgttttc tttgactgga caatcattcc ttttattcca cgtttcgctt
    14101 atcctagctg aagcgtttca gtcgattaaa tgttcgacaa ttaaccaatc agtcgcagtt
    14161 tgcgacaggt aaggtttccc cggacgattt gctggattac tctgtctggc aaatttgctc
    14221 agggaaaacc ttgcaggaga tctatgaccg tacgcgtagc gataaatggc ttcggtcgca
    14281 tcgggcgtaa tgtggttcgt gctttgtatg aatccggacg ccgggcggaa attaccgtgg
    14341 tggcaatcaa cgaactggcg gatgctgcgg gcatggcgca tttgttgaaa tatgacacca
    14401 gccatggccg ttttgcatgg gaagtacgac aggaacgcga tcaacttttt gttggtgatg
    14461 acgccatccg cgtattgcat gaacgttcac tgcaatcgct cccctggcgt gaacttggcg
    14521 ttgatgtagt cctcgactgc accggcgtat atggctcccg cgagcatggc gaagcgcata
    14581 ttgccgccgg ggccaaaaaa gtgctctttt cacatcctgg cagtaacgat ctcgacgcga
    14641 ccgttgttta cggcgtcaat caggatcaac ttcgtgcgga acaccgcatc gtttctaacg
    14701 cttcctgtac cacgaattgc ataattcccg tcatcaaatt gttagatgat gcgtacggta
    14761 ttgagtccgg cactgtgacc acaattcact ccgccatgca cgatcaacag gttattgatg
    14821 cataccatcc tgacctgcgt cgcacccggg cagccagcca gtcgatcatt ccggtcgata
    14881 ctaaactggc cgccggtatc acacgatttt ttccgcaatt taacgatcgc tttgaagcga
    14941 ttgcggtacg tgtgccaacc ataaatgtga cggcaatcga tttaagcgtg acggtgaaga
    15001 aacctgtaaa agccaatgaa gtcaacctgt tgctgcaaaa agcagcacaa ggtgcatttc
    15061 atggtatagt tgactatacg gaattgccgt tggtctctgt agattttaac cacgatccgc
    15121 acagtgccat tgtcgatggc acccaaaccc gggtcagtgg cgcacacctg atcaaaacgt
    15181 tggtctggtg cgataacgaa tggggctttg ctaaccgaat gctcgacacg acgttagcta
    15241 tggctactgt tgctttcagg taagacgcaa gcagcgtctg caaaactttt agaatcaacg
    15301 agaggattca ccatgtctgt aaataagatg accgatctgg atcttgctgg gaaacgtgta
    15361 tttatccgtg cggatctgaa cgtaccagta aaagacggga aagtaaccag cgacgcgcgt
    15421 atccgtgctt ctctgccgac catcgaactg gccctgaaac aaggcgcaaa agtgatggta
    15481 acttcccacc tgggtcgtcc taccgaaggc gagtacaacg aagaattctc tctgctgccg
    15541 gttgttaact acctgaaaga caaactgtct aacccggttc gtctggttaa agattacctc
    15601 gacggcgttg acgttgctga aggtgaactg gttgttctgg aaaacgttcg cttcaacaaa
    15661 ggcgagaaga aagacgacga aaccctgtcc aaaaaatacg ctgcactgtg tgacgtgttc
    15721 gtaatggacg cattcggtac tgctcaccgc gcgcaggctt ctactcacgg tatcggtaaa
    15781 ttcgctgacg ttgcatgcgc aggcccgctg ctggcagctg aactggacgc gctgggtaaa
    15841 gcactgaaag aacctgctcg cccgatggtg gctatcgttg gtggttctaa agtatctacc
    15901 aaactgaccg ttctggactc cctgtctaaa atcgctgacc agctgattgt tggtggtggt
    15961 atcgctaaca cctttatcgc ggcacaaggc cacgatgtgg gtaaatccct gtacgaagct
    16021 gacctggttg acgaagctaa acgtctgctg accacctgca acatcccggt tccgtctgat
    16081 gttcgcgtag caaccgagtt ctctgaaact gcaccggcta ccctgaaatc tgttaacgat
    16141 gtgaaagctg acgagcagat cctggatatc ggtgatgctt ccgctcagga actggctgaa
    16201 atcctgaaga atgcgaaaac cattctgtgg aacggtccgg ttggcgtgtt cgaattcccg
    16261 aacttccgca aaggtactga aatcgtggct aacgctatcg cagacagcga agcgttctcc
    16321 atcgctggcg gcggcgacac tctggcagca atcgacctgt tcggcattgc tgacaaaatc
    16381 tcctacatct ccactggcgg cggcgcattc ctcgaattcg tggaaggtaa agtactgcct
    16441 gcagtagcga tgctcgaaga gcgcgctaag aagtaaaaaa tcacagggca gggaaacctg
    16501 cccttgtttc agcgcgcttt tagagcacgc attttttcat actctaaata attcgagttg
    16561 caggaaggcg acaagagagt gaatccccag gagcttacat aagtaagtga ctggggtgag
    16621 cgaacgcaga cgcagcacat gcaacttgaa gtatgacgag tataaggccc gacgatacag
    16681 gacaagagac atgtctaaga tttttgattt cgtaaaacct ggcgtaatca ctggtgatga
    16741 cgtacagaaa gttttccagg tagcaaaaga aaacaacttc gcactgccag cagtaaactg
    16801 cgtcggtact gactccatca acgccgtact ggaaaccgct gctaaagtta aagcgccggt
    16861 tatcgttcag ttctccaacg gtggtgcttc ctttatcgct ggtaaaggcg tgaaatctga
    16921 cgttccgcag ggtgctgcta tcctgggcgc gatctctggt gcgcatcacg ttcaccagat
    16981 ggctgaacat tatggtgttc cggttatcct gcacactgac cactgcgcga agaaactgct
    17041 gccgtggatc gacggtctgc tggacgcggg tgaaaaacac ttcgctgcta ccggcaagcc
    17101 gctgttctct tctcacatga tcgacctgtc tgaagaatct ctgcaagaga acatcgaaat
    17161 ctgctctaaa tacctggagc gcatgtccaa aatcggcatg actctggaaa tcgaactggg
    17221 ttgcaccggt ggtgaagaag acggcgtgga caacagccac atggacgctt ctgcactgta
    17281 cacccagccg gaagacgttg attacgcata caccgagctg agcaaaatca gcccgcgttt
    17341 caccatcgca gcgtccttcg gtaacgtaca cggtgtttac aagccgggta acgttgttct
    17401 gactccgacc atcctgcgtg attctcagga atatgtttct aagaaacaca acctgccgca
    17461 caacagcctg aacttcgtat tccacggtgg ttccggttct actgctcagg aaatcaaaga
    17521 ctccgtaagc tacggcgtag tgaaaatgaa catcgatacc gatacccaat gggcaacctg
    17581 ggaaggcgtt ctgaactact acaaagcgaa cgaagcttat ctgcagggtc agctgggtaa
    17641 cccgaaaggc gaagatcagc cgaacaagaa atactacgat ccgcgcgtat ggctgcgtgc
    17701 cggtcagact tcgatgatcg ctcgtctgga aaaagcattc caggaactga acgcgatcga
    17761 cgttctgtaa gtcattcctt tctgcttatc tcaaggcccg ctctgcgggt cttttttttg
    17821 ccaaaagaaa aattgcctga tgcgctacgc ttatcaggcc tacacgtatc ctgcaatata
    17881 ttgaattagc aggtttttgt aggccggata aggcgttcac gccgcatccg gcatgacaaa
    17941 gcgcacattg atatctgtct gagccccgct ctgcgggtct ttttttcgcc aaaagcaagg
    18001 tgatacagat gagaaatctg tgatctattt ggcaaaatta tgctttattg tttacccttg
    18061 tcagactgcc cgtcataagg cggcggagtg tatttctcca ttttgagtca gttgaaaagg
    18121 aatattgaat ggaagatttg aatgttgtcg atagcataaa cggcgcggga agctggctgg
    18181 tggctaacca ggcgctgctg ctaagttatg cagtaaacat cgtggcggca ctcgcgatca
    18241 tcatcgttgg tttgattatc gcgcggatga tttccaacgc ggtgaatcgc ctgatgatct
    18301 cccgtaaaat cgatgccact gttgctgatt ttctttctgc attagtccgt tacggtatta
    18361 tcgcctttac gctaatcgct gcactgggac gcgtgggtgt acaaaccgcg tccgtcattg
    18421 ctgtactcgg tgccgcaggc ttagctgttg gtctggcttt gcaggggtca ctgtctaacc
    18481 tggccgctgg cgtgttactt gtcatgttcc gcccgttccg tgccggagaa tatgttgacc
    18541 tcggcggcgt agccggtact gtgctgagtg tgcagatttt ctccaccacc atgcgtactg
    18601 cagacggtaa aattatcgtt attccgaacg gtaaaattat tgccggaaat attattaact
    18661 tctcccgcga gccagttcgc cgtaacgaat ttattattgg cgtggcgtat gattccgata
    18721 tcgatcaggt taagcagatc ctgaccaata ttatccagtc tgaagatcgc attttgaaag
    18781 atcgcgaaat gactgtgcgc ctgaacgaac ttggtgcatc gtcgattaat ttcgtggtcc
    18841 gcgtctggag caacagcggc gatctgcaaa acgtgtactg ggatgtgctg gagcgtatta
    18901 aacgtgaatt tgatgccgcc ggtatcagct tcccgtaccc gcaaatggat gtgaacttta
    18961 agcgggtgaa agaagacaaa gctgcgtaat caacgctgag gccagataat actctctggc
    19021 ctctctctta ttattttttc tgattgccaa ttaatattat caatttccgc taataacaat
    19081 cccgcgatat agtctctgca tcagatactt aattcggaat accaaacgtg ttttcttatt
    19141 actttcaagg tcttgcactt ggcgcagcta tgatcctacc gctcggtcca caaaatgctt
    19201 ttgtgatgaa tcagggcatt cgtcgtcagt accacattat gattgcctta ctttgcgcta
    19261 tcagcgattt ggtcctgatt tgcgccggga tttttggtgg cagcgcgtta ttgatgcagt
    19321 cgccgtggtt gctggcgctg gtcacctggg gcggcgtagc cttcttgctc tggtatggtt
    19381 ttggcgcttt taaaacagcg atgagcagta atattgagtt agccagtgcc gaagtcctga
    19441 agcaaggcag atggaaaatt atcgccacca tgctggcagt gacctggctg aatccgcatg
    19501 tttacctgga tacttttgtt gtactgggca gccttggcgg gcagcttgac gttgaaccaa
    19561 aacgttggtt tgcgctcggg acaattagcg cctctttcct gtggttcttt ggtctggcta
    19621 ttctcgcagc ctggctggca ccgcgcctgc gcacggcaaa atcacagcgc attatcaatc
    19681 tggtagtggg atgtgtcatg tggtttatag ccttgcagct ggcgagagac ggtattgctc
    19741 atgcacaagc cttgttcagt taggcgctat ctgatggaaa aataaaacag aggcgctaag
    19801 cttgcctcca gaggtcctga attttgggca agtcaattaa cacggagaga ctaacgtgaa
    19861 gttcaaagtt atcgccctgg cggcattaat gggtattagc gggatggcag cgcaggctaa
    19921 cgaattgccg gatggaccgc atattgtcac ctccggtacg gcaagtgtgg atgcggtgcc
    19981 agacattgcc actcttgcga ttgaagttaa cgtggccgcg aaggatgccg ctacagccaa
    20041 gaaacaggca gatgagcgcg tcgcacaata catttccttc cttgaactca atcagatcgc
    20101 gaaaaaagat atcagctcag cgaatttacg cacccagcca gattatgatt atcaggatgg
    20161 taaaagtatc cttaaaggct accgcgctgt gagaacggtg gaagtcacgc tccgtcagtt
    20221 agacaaactg aattccttgc tggatggcgc gctgaaggcg ggtcttaacg aaattcgttc
    20281 tgtgtcgctg ggcgtggcgc agccggatgc ctataaagac aaagcgcgta aggcagcgat
    20341 tgataacgcg attcatcagg cgcaggaact ggcgaacggc tttcatcgta aactggggcc
    20401 ggtatatagc gtgcgctacc atgtttccaa ctatcagccc agcccaatgg tgcggatgat
    20461 gaaagccgat gccgcgccgg tgtccgccca ggaaacttac gagcaggccg ctattcagtt
    20521 tgatgatcag gtcgatgtgg tcttccagtt agaacctgtg gatcaacaac ccgctaaaac
    20581 acctgcagca caataatact tgtagcccga cgtattcgtc gggctaatgc ctttacccga
    20641 tattgcctcc tttttctgta attttctgta aaaccatgac gtaatcacct atctttgctc
    20701 gcggtttcat caaggatagg ctgactgaaa cggttaagcg gacaaggatg cgggggaggg
    20761 gggatggaca tttttatttc gaaaaagatg cgcaatttca ttttattagc gcaaaccaat
    20821 aatattgccc gggcggcaga aaaaatccat atgacggctt cgccatttgg taaaagcatt
    20881 gccgcgctgg aagaacaaat tggctatacg ctatttaccc gcaaagataa taatatcagc
    20941 ctcaataaag ccggacagga actgtatcaa aaactgttcc cggtttatca acggctttct
    21001 gcgattgata atgaaatcca taactcaggg cgtcgttcac gagaaattgt gattggcata
    21061 gataatactt atccgacaat tattttcgat cagttgatta gtctcggaga taagtatgaa
    21121 ggcgtgaccg cccagccagt tgagttcagc gaaaacggcg ttatcgataa tctttttgac
    21181 cgccagttag attttattat ctccccacag catgtatctg cacgagttca ggaactggaa
    21241 aacctgacta tcagtgaact accaccgctg cgattaggct ttctcgtttc tcgccgttat
    21301 gaagagcgtc aagagcaaga gctgcttcag gagctgccct ggctacaaat gcgctttcag
    21361 aaccgggcca acttcgaagc aatgattgat gcgaatatgc gaccttgtgg gatcaatcca
    21421 accattattt atcgtcccta cagttttatg gccaaaatca gcgccgtgga gcgcggacat
    21481 tttctgacgg tgatcccgca ttttgcctgg cgactggtga atccggcaac gctgaaatat
    21541 tttgatgcgc cgcacaggcc gatgtatatg caggaatatc tttattcaat cagaaatcat
    21601 cgttataccg ccacgatgct tcagcatatt gctgaagatc gtgacgggac gagtcattaa
    21661 cccagcatcg agccggttgc tattaaatta cggtgtaagt cgaagacgtg gctaagatcg
    21721 tggtgaatat gtccgccagg cgcattttcc agatagcgat gcagataatc ccgatacata
    21781 ggatgtgcac aattatcaat gatagtgcgg gcgcgttgaa gcggggaaag accgcgcaga
    21841 tcggcgatcc cttgttcagt gatgatcact ttgacgctgt gttcgctgtg atcaacatgg
    21901 ctgcacattg gcacgacggt tgagatcttg ccttctttag caatcgacgg ggccataaag
    21961 atcgacagat acgcgttgcg ttcaaaatca ccgctgccgc cgatgccgtt catcagatcg
    22021 accccggcta cgtgcgttga gttggcatgc ccgtaaatat caaactccag gccgacgttc
    22081 agagcgatga cgcccagacg acggatgatt tccgggttat tggaaatctc ctgcggacgc
    22141 aacacaatgc ggctggcaaa gtaatccata ttgtcgtaaa tcttgcgcag ggaatcggcc
    22201 gagattgtca ggctggaggc gctggccccg ctgattttgc cggtttccag taaatgcacc
    22261 accgattcct gtagcacttc cgaatacatc ataaacggag gaatttccgg gttttccccc
    22321 agacgcgcca ttaccgcatt attgatattg cccacgccac tttgcagcgg cagaaattcc
    22381 ggcggaatac gcccatgcgc catttcctgc aacaagaacg tgaccacgtt atcggcaatc
    22441 tgctggcaca tgggattttg cttatccagc atattaccgg cgtcgggcaa gttggtttcc
    22501 acgacggcga caatcttttt cggatcgatt tgcacatagc gggtaccgac gcgatccatt
    22561 gcatggaaga tcgacacgct attgcgccgc ggtggcgcgc caggaatcac aatatctgcc
    22621 agttctgcaa cgcgcggatc gtgatagtga ttaagttcaa tgatcacttt cttcgctcgc
    22681 agcagccagg tcggcgcatt accgatcccg ctggttaacc agactcgacc atctggtgcc
    22741 agtgccgatg cttcaatgac ggcaacatca atgtcgccga agaaaccgta attgaccatt
    22801 tgcgccactt cgctcaaatg caggtcaacg aaactcaccg cgccctgatt gatcttttta
    22861 cgtaaaccgg acgatgtttg atatggcgca cgccaggaaa cagcatcggc gtcagaaagt
    22921 acatcgtcag cggcggcgct gattgacgcc cccgtcagca ggcgaatttg atacggcttt
    22981 ttggcctcat gctgttcgtt agctctgcgg gcaatcgcgg tgggtagggc tttcggcgaa
    23041 cccgctgggg taaaaccgct aaatgccacc atgtcgttat gctggataat ttctgccgct
    23101 tcatcggcgg tcatccttgt ccactgagtt tccattttta ctccttcgtt cgcagggatt
    23161 aatgaccaac gaaattaggt ttacgttttt cgaggaaagc gttcatccct tcctggtaat
    23221 cttcgctgtc ataaaccgcg cggcgcatcc cctgaatacg ttcaaattca tcggagttca
    23281 tggtgtgtgc ttcgcccagt acacgcagct cttctttgat aacggcaatg gctaacggcg
    23341 ctttctcaga gatgtggtgc gccatttgta aggtgaaatc ttccagttct tccacttcca
    23401 caacatggtt gaggatgccg acagccagcg cgcgctgggc ggtgattggc gaagcggtaa
    23461 aaatcagctc tttgacaatg tggaagcccg cgtcgcgggt caggttgtga atgccgacca
    23521 ggttatacgg gacgccgagg tttacaggcg tcattgagaa ggttgaggta ctggcggcga
    23581 tgatcagatc ggaactcatg atcatttcaa atgcgccacc ccaaacacta ccttccacca
    23641 tcgaaatgat cggtttcggg aatttttgga tcatgcgggt gatttgacgc aacggatcat
    23701 cataggagag cggatcgcga ccgccagacg gcagttcgtg aatatcgtga cctgcggaga
    23761 agactttgga tccactcggc gcgcgcaaaa tgatacagcg aatttccggc cggttgagat
    23821 cgcttaacgc ctgcataaga tcatcaataa agactttact taaggcatta agttttcggc
    23881 catagttaaa ctcaatgacc gccactttgt tgatagtggc aacgttcaca tactgataag
    23941 acataaaaat tcctttaatc aaaatattgc gtctggataa attcactgag ctgccgcagg
    24001 ccggtgcgcg gtgagagcgt attgtttttg accgctaaaa gcgtctggcg gtaatagcga
    24061 tcgaaatctt cattcgcgaa caggtgattc agtacttctt cttcggtctg cttacgcagc
    24121 cattccaccg attgttgttg ccgcacttgt tgtaaacgac cactggcagt tagcgcggtt
    24181 ttgaagtcga tgatggcgtg ccagatctca tcgattccac gtttttccag tgcgctacaa
    24241 gtcagaaccc gtggctgcca ttcgtcgtat ttacgtcgca gaatatgcag ggcactctcg
    24301 tacatatgcc gggcaatggc gacattggta tggttatcgc catcgtcttt gttgataacg
    24361 atcagatcag ccacttccat cagccctttt ttaatgccct gcagatcatc gccgccaccg
    24421 gcaatttgca acgagataaa acagtccacc atgcgggcga cttccgtttc cgactgcccg
    24481 acgccaaccg tttcgacaat cactacgtca taacccgccg cttcgcataa cagcattaat
    24541 tcccgcgctc gctgactggc accgcccaga tgaccggagg atggtaccgg gcgaataaac
    24601 gccgtctcgg cacgcgccag gtcattcatg cgggttttat ccccgagaat gctaccgcca
    24661 gtgaccggac tgctgggatc gaccgcaata accgcaacct ttaatccatc acgaatcaac
    24721 aacatgccaa aggcttcaag aaaggtactt ttccccgcgc cgggtgtgcc ggtaacgccc
    24781 agtcgcaggg cgttaccgca gtacggcata atggcatcaa gcagttgcgt acttagcgcc
    24841 tgatgacgcg ggtgacggct ttccaccagc gtcatggcct gggcgagtgt ggcacgctca
    24901 ccctgacgta agcggcgaat actttctgcc agcgtggctt cattaatcat gatgctggct
    24961 tatcagattc agtacgtcgc gcacactgtc gagcataggt gtacctggac cataaatcgc
    25021 cgccacgccg cgctcttgca ggaaggcgta atcctgcggt ggaatgacgc caccggcgac
    25081 tacgcagata tcttcgcgtc cccatttttt cagcgcttcg accagttccg ggatcagcgt
    25141 tttatgaccg gcagccagtg aggatgcgcc cactacgtga acgtcatttt ctactgccag
    25201 gcgggcgatc tcttcaggtg tagagaacat cgggcttaaa tctacgtcga aaccgagatc
    25261 ggaataggcg ctggcgatca ctttcgcgcc gcgatcgtgc ccatcctggc ccattttggc
    25321 gatcagaatg cgcgggcgac gaccattgtc ggcaaggaac tgctccgttt gcgcaacaat
    25381 ggcatcgaac tccgaggccg atttctcaga ttgatgatag ctttgcgcaa tcacgccggt
    25441 aacacactgg cttggcacca gataacggtc gaaagcggct tccagcgcat cggaaatttc
    25501 acccagggtg gcgcgaacgc gagcggcatt aacagcggca gccagcaggt tttcgttatg
    25561 ctgtgcggcg tgagtcaggg cgttcaacgc ggcggttacg gcggcatcat cacgggtggc
    25621 gcgaatgcgt tccagcgaag caatttgctc gttacgcacc atcacgttgt cgatctcaag
    25681 tacatcggtt tcgtcttcgt gatccagttt gtacttgttg acaccaacga tgacacgctt
    25741 gccctggtcg atcagcgact gttcgcgcgc tgaggcctct tcgatcattc gttttggcag
    25801 acctgcttcg atcgctttcg ccatgccacc ggcttcgtcg atctgttgga taatagctct
    25861 ggcttgtttg acgatttgat cggtcagcga ctcaatgtaa taggatccgg ccagtggatc
    25921 gacggtgcgg cagagttctg attcttcctg gatgatgatc tgggtgttgc gggcaatgcg
    25981 tgcggagaaa tcggtaggca aaccaagcgc ttcgtcaaag gcgttggtat gcagtgactg
    26041 agtaccgccc agcgtcgcag ccagtgcttc aatggtggtg cggataacgt tgttatacgg
    26101 atcctgttca gtcaggctcc agcctgaggt ctggcagtgg gtacgcagcg ccagtgattt
    26161 cgggtcctgt gcgccaaatc cactgaccgc ttcgctccat aaataacgtg ccgcacgcaa
    26221 catggcgacg ttcataaaca gatccatgcc gatgccgaag aagaacgaca ggcgaggagc
    26281 gaagtcatca attttcagtc cggcagagat tgctgctttg atgtactcaa tcccatcagc
    26341 gagcgtaaat gctacctgct gcacgcagtt ggcacccgct tcacccatgt ggtaaccgct
    26401 gatactgatg gtattaaatc gcggcatgtt gccggaacac caggcgatga tgtcggcgat
    26461 aatgcgcatt gacggttttg gcgggtaaat ataggtgttg cggcagaggt actctttgag
    26521 gatatcgttc tgaatggtgc cggtcagttt atcaggtgta acaccttgct cttccgcggc
    26581 gacgatataa aacgccagta ctggtagcac tgcgccattc atggtcatcg aaaccgacat
    26641 tttatccagc gggatctggt cgaacaggac tttcatatct tccacggtgt cgatagcgac
    26701 gcccgctttg ccgacgtcgc ccgccacgcg cgggttatcg gagtcgtagc ctcggtgggt
    26761 ggcaaggtca aacgcaacgg aaagaccttt ttgcccggcg gccaggttac ggcgataaaa
    26821 agcgttggac tcttttgctg ttgaaaaacc agcatactga cggatggtcc acggttgggc
    26881 ggtatacata gtggcacgcg ggccacgaac gtagggcggc aaaccaggaa gggtacctgt
    26941 cacctccaga ttatcgagat cggcttcggt atacagcggc ttgatggcga tcccttccgc
    27001 ggtttgctga accagcgagt cgacagtttt ctcccgacgg ctcaattcct tgttggcaag
    27061 ctgttgccac tcctgcacgt tagacattat ttgctccgta aaacgcaatc aaaatgaggg
    27121 tatttgcgcc tacggtgagc tttttacgtg ggtttgtcgc cagcaaaaaa aatgcattca
    27181 gacgtcgttt tttgccgcct gagatttgtc tattttgtga tgtgattcaa ggaatacaaa
    27241 aagaagtgag gacaacgcct gatatgtgct atcaggcatt atttgatgga ttaatcctga
    27301 cgaaggactt tgtgaccata atcgagtaac gcatcagtga ctttacgcat catgcggctt
    27361 tcaggagcaa agcggtgcca gtagagcatc cgtcgttgaa atagcccagg cgttaagtca
    27421 atcagttcac cgctggccag ctctttctcg atttgcaggt gcgggatcat acagcaggtg
    27481 gtgccctggc gagcaagttg tacgaacgct tctgaagaat taacgatatg gcagggcacg
    27541 ctgcctggag gcagatcgaa gttttgctgc aaaaaggcct ggtgcatatc gtcaagatgg
    27601 tcaaacgcga ccactggcgc tttcagtaat gccgaacgcg ttacgccgtt agggaaatat
    27661 ttttcggcaa agggttttga gctgacgaac agatagtcga gcgcaccaag tttatcgaca
    27721 agacaactcg gcagcgcctg atgttgaata ctcaccgcgc cgaccacttc gccgcggcgc
    27781 agacgttcct gagtgcgggt ttcatcttct acctgcaagt tgaggcggat aggcgaatca
    27841 gccaacacag gagccagtgc aggaagcaac cacgtcgcca gactgtcggc gttgaccgcc
    27901 agtgaaagca gcagcggagt cgaaccggtt tgttcatcgc ccagccactc ttcttccagc
    27961 aactccacct ggcgcagcag tgccagcagt ttttgacctt gttccgttgg gcgcggcggt
    28021 acggtacgca ctaacagcgg ctgcccgaac atattttcca gttgcttaat gcgctgtgag
    28081 acggctgatt gtgtaatgca cagcttttgt gccgcgcgct caaatcctcg ttcacgtatc
    28141 accgcatcca gtgcctgtaa tgttctgtag tccgggcgtt tcattgctgt tgtctgctcc
    28201 tggtaaatgc tttttcctgc actatgacac aattttgtgt caggttgcat ataccgaagc
    28261 cgtcggcggg taacatttgt attcgtggtt aaacgcgcag cttaaaagat cagggatttg
    28321 cgctaatgca tttcgctcag cgagttcgcg ccctggttgt gttgaatggc gtggcgttat
    28381 tgcctcaatt tgcctgtaaa caggggcttg cgaacggtga actggtgcgc ctgtttgcac
    28441 cgtggagcgg catacccaga ttgttgcatg ccttatttgc ggggcgaaag gggatgcctg
    28501 ccattgcgcg atattttatg gatgagttaa ccacgcggct tgccaacggg gtctgaatcg
    28561 ctttttttgt atataatgcg tgtgaaattt cataccacag gcgaaacgat catgacgcag
    28621 gatgaattga aaaaagcagt aggatgggcg gcacttcagt atgttcagcc cggcaccatt
    28681 gttggtgtag gtacaggttc caccgccgca cactttattg acgcgctcgg tacaatgaaa
    28741 ggccagattg aaggggccgt ttccagttca gatgcttcca ctgaaaaact gaaaagcctc
    28801 ggcattcacg tttttgatct caacgaagtc gacagccttg gcatctacgt tgatggcgca
    28861 gatgaaatca acggccacat gcaaatgatc aaaggcggcg gcgcggcgct gacccgtgaa
    28921 aaaatcattg cttcggttgc ggaaaaattt atctgcattg cagacgcttc caagcaggtt
    28981 gatattctgg gtaaattccc gctgccagta gaagttatcc cgatggcacg tagtgcagtg
    29041 gcgcgccaac tggtgaaact gggcggtcgt ccggaatacc gtcagggcgt ggtgaccgat
    29101 aatggcaacg tgatcctcga cgtccacggc atggaaatcc ttgacccgat agcgatggaa
    29161 aacgccataa atgcgattcc tggcgtggtg actgttggct tgtttgctaa ccgtggcgcg
    29221 gacgttgcgc tgattggcac acctgacggt gtcaaaacca ttgtgaaatg atctgacggg
    29281 ggaacctccc ccgttaaaaa aattctcttc attaaatttg gtgacatgtg tcacgctttt
    29341 accaggcaat tgtcgattgc tctaaataaa tcctctaaac cagcatattc atccaagaat
    29401 tacctttgcg tgatatttcc tcaacatcgc gacgcaaacg ttcatattgc cgcaatatta
    29461 ttttttgata tgttgaaagg cggatgcaaa tccgcacaca acatttcaaa agacaggatt
    29521 gggtaaatgg caaaggtatc gctggagaaa gacaagatta agtttctgct ggtagaaggc
    29581 gtgcaccaaa aggcgctgga aagccttcgt gcagctggtt acaccaacat cgaatttcac
    29641 aaaggcgcgc tggatgatga acaattaaaa gaatccatcc gcgatgccca cttcatcggc
    29701 ctgcgatccc gtacccatct gactgaagac gtgatcaacg ccgcagaaaa actggtcgct
    29761 attggctgtt tctgtatcgg aacaaaccag gttgatctgg atgcggcggc aaagcgcggg
    29821 atcccggtat ttaacgcacc gttctcaaat acgcgctctg ttgcggagct ggtgattggc
    29881 gaactgctgc tgctattgcg cggcgtgccg gaagccaatg ctaaagcgca ccgtggcgtg
    29941 tggaacaaac tggcggcggg gtcttttgaa gcgcgcggca aaaagctggg tatcatcggc
    30001 tacggtcata ttggtacgca attgggcatt ctggctgaat cgctgggaat gtatgtttac
    30061 ttttatgata ttgaaaacaa actgccgcta ggcaacgcca ctcaggtaca gcatctttct
    30121 gacctgctga atatgagcga tgtggtgagt ctgcatgtac cagagaatcc gtccaccaaa
    30181 aatatgatgg gcgcgaaaga aatttcacta atgaagcccg gctcgctgct gattaatgct
    30241 tcgcgcggta ctgtggtgga tattccggcg ctgtgtgatg cgctggcgag caaacatctg
    30301 gcgggggcgg caatcgacgt attcccgacg gaaccggcga ccaatagcga tccatttacc
    30361 tctccgctgt gtgaattcga caacgtcctt ctgacgccac acattggcgg ttcgactcag
    30421 gaagcgcagg agaatattgg cctggaagtt gcgggtaaat tgatcaagta ttctgacaat
    30481 ggctcaacgc tctctgcggt gaacttcccg gaagtctcgc tgccactgca cggtgggcgt
    30541 cgtctgatgc acatccacga aaaccgtccg ggcgtgctaa ctgcgctgaa caaaatcttc
    30601 gccgagcagg gcgtcaacat cgccgcgcaa tatctacaaa cttccgccca gatgggttat
    30661 gtggttattg atattgaagc cgacgaagac gttgccgaaa aagcgctgca ggcaatgaaa
    30721 gctattccgg gtaccattcg cgcccgtctg ctgtactaat tccccttctc tgaaaatcaa
    30781 cgggcaggtc actgacttgc ccgttttttt atcccttctc cacaccgttg ccgcgttgcg
    30841 cattctactt tgcgagtctg ctccagaaaa taaatcaaag tcagatcaat gcgttgtgtt
    30901 tatttaatgg ggatttacga tggcaggaca gcatagggct gtaacggtaa agccctcacc
    30961 gaagcgaggg cttgaaggag aagggttatg atgcgacttg tcatcatact gattgtactg
    31021 ttactcataa gtttcccagc ttattaacag tcagtctcag gggaggagtc atcctccctt
    31081 acccttactc actaaattag gtcaaagaat caacgatgtc aatcagggcg atgcgggatg
    31141 tatcgccctg aaccgatcat ttgatagaca cttcatcgcc cacgtttact gccgtaatta
    31201 atcccttatc cctgcaatgt gtgtctgtct tacatgacgt tttacgagtg cgctcgaaga
    31261 aaaaatttaa aggtagatca atgcattgtg catatttaaa gagagtttac gatggcaggg
    31321 cagcatgggg ctgtaacggt aaagccctca ccgaagcgag ggcttgaagg agaagggtta
    31381 tgatgcgact tgtcatcata ctgattgtac tgttactcat aagtttcagc gcttattaac
    31441 agtcagtcac aggggaggag caatcctccc ttacccttac tcactaaatt aggttaaaga
    31501 atcaacgatg tcaatcaggg cgatgcaggt gtgtcgccct caccactccc agactttcga
    31561 cggtgtaacc accgcaggaa gagggatatc ccactcttca acggggagtt tttccaccaa
    31621 ctgacaatca tgcgcataac ccaccggttg cattttatag tgctgccagt tttgtaaggt
    31681 ccgatcataa aaaccaccgc ccattcccag gcgctggccg tactcatcaa aggcgaccag
    31741 cggtgtgatc aacacgtcta atcgggaaag gggtagcacg tcacgcacat ccaattttgg
    31801 ctcatggatc ttcaacctgt tcatcaccag ttcgctttgc ggatggtaat tcaggaacag
    31861 caaattaccg gcactaaagg gatgcaaaac tggaagatat acgcgcttac cggcgcgcca
    31921 gagttgttct atgagtggct gggtgtcgag ttcgccatca aaagagagga atacagcgac
    31981 cgtatgtgcc atcaccaccg ggggataagt catcatccgg gtagcggctt gttgacccat
    32041 ttcctgctgt tgttccggcg ttaacgtacg acgacgttgc cgaatcattt tgcggatttc
    32101 ttgtcgggat aatgtcagtg ggagttctgg tagttgcgtc atggctggtg ccagataaga
    32161 agggaatctc cgagatgccg ccgcaggctg taacccttga acccttggtt caaggtgaat
    32221 gtgtcgtcgc agttttaagg cttctcggac ggaccgagca tgctcaccaa ccgcggagcg
    32281 ccacattctt gtggtatgaa atatcggctc aggggactgg cccgcttgcg aacatctcag
    32341 agaaattttg tcttcacggt tactctacca cagtaaaccg aaaagtgtca ttcaaagttt
    32401 tggttagttt tttcggtgat gcgaccttgt tcaagtaacg cttgttctat ggtctgctgc
    32461 agcatccgaa tacgctgttc catacttgcc gcgtactcac gagtcttcgc tttttcttgc
    32521 gctaactcat agctgatatt caatgcggca atgaagacca actgttcagt atttgtgact
    32581 ctagtgcgtt ctttcagatc ttgcaaccgt tggttcagat cgtccgctgc ctgattcaac
    32641 gcatcccttt ggtcaggcgg gcagttcaca cgcagtgaac ggccaaaaat ttggatatcg
    32701 acgggttgtg cagacatgcc accttcctgc tgattgactg cgctgccttc gtctttagac
    32761 ccatggtctg cgaaggggcg acactatagc taccctgatg agaagagaca agcccttttc
    32821 tggtccacca gggcccaaag tggtagcata tcatgaatat tcctcccttt gacgacgaat
    32881 gcttatgtct atacagaacg aaatgcctgg ttacaacgaa atgaaccagt atctgaacca
    32941 acaagggacg ggtctgaccc ctgctgagat gcatggttta atcagtggga tgatatgtgg
    33001 cggtaacgat gacagctcat ggctgccgct acttcacgac ctgacgaacg aaggcatggc
    33061 tttcggtcat gagctggcac aggcactgcg taaaatgcac tctgccacca gcgatgccct
    33121 gcaggatgac ggcttccttt ttcagcttta tctgcctgat ggcgatgatg tcagcgtttt
    33181 cgatcgggct gatgcgctgg caggttgggt caatcacttc ctgcttggtc ttggcgttac
    33241 gcaaccgaag ctggacaaag tgaccggcga aaccggtgaa gccatcgacg atctgcgtaa
    33301 catcgcgcag ttgggctacg acgaagacga agatcaggaa gagcttgaaa tgtcgcttga
    33361 agagatcatc gagtacgtcc gtgttgccgc gctgttatgc cacgacacct ttactcatcc
    33421 gcaaccgacc gcgccagaag tacaaaaacc gactctacac taaaaacaaa aaacgtaagg
    33481 agagtgttat gagtgagata tcccggcaag agtttcagcg tcgccgtcag gccctggtgg
    33541 agcaaatgca acccggcagc gccgcgctga tttttgctgc accagaagta acacgtagcg
    33601 ccgacagcga atacccctat cgtcagaaca gtgacttctg gtacttcacc ggctttaacg
    33661 aaccggaagc ggtgctggtg ctgattaaaa gcgatgacac tcataaccac agcgttctgt
    33721 ttaaccgcgt tcgcgacctg acggcggaga tctggtttgg ccgtcgttta ggtcaggatg
    33781 ccgcgccaga gaaactgggc gttgaccgcg cactggcatt cagcgaaatt aatcagcaac
    33841 tttatcaact acttaatggt ctggatgtgg tttaccacgc ccagggcgaa tatgcatatg
    33901 ctgatgaaat cgtgaacagc gcgcttgaga aactgcgtaa aggttcgcgg caaaatctca
    33961 ccgcgccggc aacgatgatc gactggcgtc ccattgttca tgaaatgcgc ctgttcaaat
    34021 cgccggaaga gattgccgta cttcgccgcg cgggtgaaat caccgccctg gcgcataccc
    34081 gggcgatgga aaaatgccgt ccgggaatgt tcgagtacca cctggaaggc gaaattcacc
    34141 acgaatttaa ccgccacggc gcgcgctatc cttcctataa caccattgtc ggcagcggtg
    34201 aaaacggctg cattctgcat tacaccgaaa acgagtgtga actgcgcgac ggcgatctgg
    34261 tgttgattga cgcgggttgc gaatacaaag gttacgctgg cgatattacc cgcaccttcc
    34321 cggtcaacgg caaattcact caggctcagc gtgaaatcta cgacattgtg ctggagtctc
    34381 tcgaaaccag cctgcgcctg tatcgtccgg gaacttccat tcaggaagtc accggtgaag
    34441 tggtgcgcat catggttagc ggcctggtaa aactcggcat cctgaaaggt gatgttgatg
    34501 aactgatcgc tcagaacgcc catcgtcctt tctttatgca tggccttagc cactggttag
    34561 ggctggatgt ccatgacgtt ggcgtttatg gtcaggatcg ctcgcgcatt ctggaaccgg
    34621 gcatggtact gaccgtagag ccagggctgt atattgcgcc ggatgcagaa gtgccagaac
    34681 aatatcgcgg tatcggcatt cgtattgaag acgacattgt gattaccgaa accggtaacg
    34741 aaaacctcac cgccagcgtg gtgaaaaagc cggaagaaat cgaagcattg atggcagctg
    34801 cgagaaagca atgagcgtaa tcatcgtcgg tggcggcatg gcgggcgcga cgctggcgct
    34861 ggctatttcc cggttaagtc acggggtgtt gccggtacat ctgattgaag cgatcgcacc
    34921 agagtcacat gcccatccag gctttgatgg ccgggctatt gcgctggcag caggcacttg
    34981 tcagcagctg gcgcgcatcg gtgtctggca atctctggcc gattgcgcaa ccgccatcac
    35041 caccgttcat gtcagcgatc gcggccatgc cggattcgtc actcttgccg cagaagatta
    35101 tcaactggcg gcgctagggc aggttgtcga attgcacaat gttgggcaac ggctgtttgc
    35161 gctgttgcgt aaagcaccag gcgtcacgct gcattgtccg gatcgcgtgg ctaacgttgc
    35221 ccgtactcag agtcatgttg aagtgacgct ggagtgtggc gagacgctaa cgggccgcgt
    35281 gctggtagcc gctgatggca cccattcaac gttagccact gtgtgcggcg ttgactggca
    35341 gcaggagcct tacgaacaac tggctgtgat tgccaacgtt gcgacttccg ttgcgcatga
    35401 agggcgagct tttgaacgct ttacgcaaca tggcccgctg gcgatgttgc cgatgtctga
    35461 cggacgctgt tcgctggtct ggtgtcatcc actggaacgg cgcgaagagg tgctgtcgtg
    35521 gagcgacgag aagttttgcc gtgaactcca gtcggccttt ggctggcgac ttgggaaaat
    35581 tacccacgct ggtaaacgca gtgcttatcc gctggcgtta acccacgccg ccagatctat
    35641 tacccatcgt accgtgctgg tgggcaatgc ggcgcaaacc ctgcacccga ttgccgggca
    35701 agggttcaac ctcggtatgc gagatgtcat gagtcttgcg gaaaccctga ctcaggcgca
    35761 ggagcgcgga gaagacatgg gtgattacgg cgtattgtgt cgttatcagc agcgtcgaca
    35821 gagcgatcgc gaagcaacca ttggcgtcac ggacagcctt gtacatcttt ttgccaaccg
    35881 atgggcgccg ctggttgtcg ggcgcaacat cgggctgatg acgatggaat tatttacccc
    35941 ggcacgcgat gtgctggcgc agcgcaccct cggttgggtg gcgcgttgaa ggcggtttta
    36001 aggaataaac aatgcaaagt gttgatgtag ccattgttgg cggcggcatg gtggggctgg
    36061 cggttgcctg cggcttacag ggtagcggct tacgcgttgc cgtactggag cagcgcgtac
    36121 cggaacctct ggcggcggat gcaccaccac aactgcgcgt ttcggctatc aatgccgcca
    36181 gcgaaaaatt actcacccgt cttggcgtct ggcaggacat tctctctcgt agggccagtt
    36241 gttatcacgg catggaagtg tgggacaaag acagttttgg tcacatttcg tttgacgatc
    36301 aaagcatggg ctatagccat cttgggcata tcgttgaaaa ctcagtgatt cactacgcgc
    36361 tgtggaacaa agcgcatcag tcgtcagata tcactctgtt ggcccccgca gaattacagc
    36421 aggtcgcctg gggagaaaac gaaaccttcc tgacgctgaa agatggcagc atgttaacgg
    36481 cgcgtctggt gattggtgcg gatggcgcta attcctggtt gcgcaacaaa gccgatattc
    36541 cgctgacttt ctgggattat cagcatcacg cgctggtggc gaccattcgc acggaagaac
    36601 cgcatgatgc ggtggcgcgg caggttttcc acggcgaagg cattctggcc tttttgccgc
    36661 ttagcgatcc gcatctttgc tcgattgtct ggtccctatc gccagaggaa gcgcagcgga
    36721 tgcagcaggc aggggaaggc gaatttaatc gcgcgttaaa tatcgctttt gataatcgcc
    36781 tgggcttatg caaggtcgag agcgagcgtc aggtgttccc gctgacgggg cgttatgcgc
    36841 gccagtttgc cgcacatcgt ctggcgctgg tgggcgacgc cgcgcatacc attcacccac
    36901 tggcggggca gggcgttaat ctcggcttta tggacgccgc agagctgatt gctgaactga
    36961 aacggttgca tcgtcagggt aaagacatcg ggcagtacat ttatctgcgc cgctatgagc
    37021 gtagtcgcaa gcacagtgcg gcactgatgc tggcaggtat gcagggcttt cgcgatctct
    37081 tttccggcgc taatccggcg aaaaaactgc tgcgcgatat tggtctgaaa ctggctgata
    37141 cacttcctgg cgttaagccg caacttatcc gtcaggcaat gggattaaac gatttgcctg
    37201 agtggctgcg ttaaaaattt ctcttctgct gtttatttga tacgcatcac actttcatct
    37261 cccggttttt tcgccgggag attttcctca tttgaaataa actaatttca cctccgtttt
    37321 cgcattatat tttctaatgc catgattttt tgatttagtg ttttttgaca tttttttagc
    37381 tcttaatatt gtcttattca aattgacttt ctcatcacat catctttgta tagaaactgg
    37441 tgtgtttttt ggttttttat tctgtcgcga tttttgcatt ttttaaccat aagctaatgt
    37501 gatgatcaat tttaccttat ggttaacagt ctgtttcggt ggtaagttca ggcaaaagag
    37561 aacgattgcg ttggggaccg ggagtggctc cgatgctggg tttcgtggtg ataatttcac
    37621 catgaaaagt tgtcagcccc gcttattcaa tgaggacaag atggcacaac agactccttt
    37681 gtacgaacaa cacacgcttt gcggcgctcg catggtggat ttccacgggt ggatgatgcc
    37741 gctgcattac ggttcgcaaa tcgacgaaca tcatgcggta cgtaccgatg ccggaatgtt
    37801 tgatgtgtca catatgacca tcgtcgatct ccgcggcagc cgcacccggg agtttctgcg
    37861 ttatctgctg gcgaacgatg tggcgaagct caccaaaagc ggcaaagccc tttactcggg
    37921 gatgttgaat gcctctggcg gtgtgataga tgacctcatc gtctactact ttactgaaga
    37981 tttcttccgc ctcgttgtta actccgccac ccgcgaaaaa gacctctcct ggattaccca
    38041 acacgctgaa cctttcggca tcgaaattac cgttcgtgat gacctttcca tgattgccgt
    38101 gcaagggccg aatgcgcagg caaaagctgc cacactgttt aatgacgccc agcgtcaggc
    38161 ggtggaaggg atgaaaccgt tctttggcgt gcaggcgggc gatctgttta ttgccaccac
    38221 tggttatacc ggtgaagcgg gctatgaaat tgcgctgccc aatgaaaaag cggccgattt
    38281 ctggcgtgcg ctggtggaag cgggtgttaa gccatgtggc ttgggcgcgc gtgacacgct
    38341 gcgtctggaa gcgggcatga atctttatgg tcaggagatg gacgaaacta tttctccttt
    38401 agccgccaac atgggctgga ctatcgcctg ggaaccggca gatcgtgact ttatcggtcg
    38461 tgaagccctg gaagtgcagc gtgagcatgg tacagaaaaa ctggttggtc tggtgatgac
    38521 cgaaaaaggc gtgctgcgta atgaactgcc ggtacgcttt accgatgcgc agggcaacca
    38581 gcatgaaggc attatcacca gcggtacttt ctccccgacg ctgggttaca gcattgcgct
    38641 ggcgcgcgtg ccggaaggta ttggcgaaac ggcgattgtg caaattcgca accgtgaaat
    38701 gccggttaaa gtgacaaaac ctgtttttgt gcgtaacggc aaagccgtcg cgtgatttac
    38761 ttttttggag attgattgat gagcaacgta ccagcagaac tgaaatacag caaagaacac
    38821 gaatggctgc gtaaagaagc cgacggcact tacaccgttg gtattaccga acatgctcag
    38881 gagttgttgg gcgatatggt gtttgttgac ctgccggaag tgggcgcaac ggttagcgcg
    38941 ggcgatgact gcgcggttgc cgaatcggta aaagcggcgt cagacattta tgcgccagta
    39001 agcggtgaaa tcgtggcggt aaacgacgca ctgagcgatt ccccggaact ggtgaacagc
    39061 gaaccgtatg caggcggctg gatctttaaa atcaaagcca gcgatgaaag cgaactggaa
    39121 tcactgctgg atgcgaccgc atacgaagca ttgttagaag acgagtaacg gctttattcc
    39181 tcttctgcgg gagaggatca gggtgaggaa aatttatgcc tcaccctcac tctcttcgta
    39241 aggagagagg ttcacaattc actgcacgtt tcaggaacca tcgctcatga cacagacgtt
    39301 aagccagctt gaaaacagcg gcgcttttat tgaacgccat atcggaccgg acgccgcgca
    39361 acagcaagaa atgctgaatg ccgttggcgc acaatcgtta aacgcgctga ccggccagat
    39421 tgtaccgaaa gatattcagc ttgcgaccgc gccgcaggtt ggcgcaccgg cgaccgaata
    39481 cgccgcactg gcagaactca aggctattgc cagtcgcaat aaacgcttca cgtcttacat
    39541 cggcatgggt tacaccgccg tgcagctacc gccggttatc ctgcgtaaca tgctggaaaa
    39601 tccgggctgg tataccgcgt atactccgta tcaacctgaa gtctctcagg gccgccttga
    39661 agcactgctc aacttccagc aggtaacgct ggatttaacc gggctggata tggcctctgc
    39721 ttctcttctt gacgaagcca ccgctgccgc cgaagcaatg gcgatggcga aacgcgtcag
    39781 caaactgaaa aatgccaacc gcttcttcgt ggcttccgat gtgcatccgc aaacgctgga
    39841 tgtggtccgt actcgtgccg aaacctttgg ttttgaagtg attgtcgatg atgcgcaaaa
    39901 agtgctcgac catcaggacg tcttcggcgt gctgttacag caggttggca ctaccggtga
    39961 aattcacgac tacactgcgc ttatcagcga actgaaatca cgcaaaattg tggtcagcgt
    40021 tgccgctgat attatggcgc tggtgctgtt aactgcgccg ggtaaacagg gcgctgatat
    40081 tgtttttggt tcggcgcaac gctttggcgt accgatgggt tacggtggcc cacacgcggc
    40141 attctttgcg gcgaaagacg aatacaaacg ctcaatgcca ggccgtatta tcggtgtatc
    40201 gaaagatgca gctggcaata ccgcgctgcg catggcgatg cagactcgcg agcaacatat
    40261 tcgtcgtgag aaagcgaact ccaacatttg tacttcccag gtgctgctgg caaacatcgc
    40321 cagcctgtat gccgtttatc acggcccgat tggcctgaaa cgtatcgcta accgcattca
    40381 ccgtctgact gatatcctgg cggcaggcct gcaacaaaaa ggtctgaagc tgcgccatgc
    40441 gcactatttc gacactctgt gtgtggaagt ggccgacaag gcgggcgtac tggcgcgtgc
    40501 cgaagcggct gaaatcaacc tgcgtagcga tatcctgaac gcggttggga tcacccttga
    40561 tgaaaccacc acgcgcgaaa acgtgatgca gcttttcagc gtactactgg gggataatca
    40621 cgggctggac atcgacacgc tggacaaaga cgtggctcac gacagccgct ctatccagcc
    40681 tgcgatgctg cgcgacgacg aaatcctcac ccatccggtg tttaatcgct accacagcga
    40741 aaccgaaatg atgcgctata tgcactcgct ggagcgtaaa gatctggcgc tgaatcaggc
    40801 gatgatcccg ctgggttcct gcaccatgaa actgaacgcc gccgccgaga tgatcccaat
    40861 cacctggccg gaatttgccg aactgcatcc gttctgcccg ccggagcagg ccgaaggtta
    40921 tcagcagatg attgcgcagc tggctgactg gctggtgaaa ctgaccggtt acgacgccgt
    40981 ttgtatgcag ccgaactctg gcgcacaggg cgaatacgcg ggcctgctgg cgattcgtca
    41041 ttatcatgaa agccgcaacg aagggcatcg cgatatctgc ctgatccctg cctctgcgca
    41101 cggcactaac ccggcttccg cgcatatggc aggaatgcag gtggtggttg tggcgtgtga
    41161 taaaaacggc aacatcgatc tggctgatct gcgcgcgaaa gcggaacagg cgggcgataa
    41221 cctctcctgt atcatggtga cctatccttc cacccacggc gtgtatgaag aaacgatccg
    41281 tgaagtgtgt gaagtcgtgc atcagttcgg cggtcaggtt taccttgatg gcgcgaacat
    41341 gaacgcccag gttggcatca cctcgccggg ctttattggc gcagacgttt cacaccttaa
    41401 cctgcataaa actttctgca ttccgcacgg cggtggtggt ccgggtatgg gaccgatcgg
    41461 cgtgaaagcg catctggctc cgtttgtacc gggtcatagc gtggtgcaaa tcgaaggcat
    41521 gttaacccgt cagggcgcag tttctgcggc accgttcggt agcgcctcta tcctgccaat
    41581 cagctggatg tacatccgca tgatgggcgc agaagggctg aaaaaagcaa gccaggtggc
    41641 aatcctcaac gccaactata ttgccagccg cctgcaggat gccttcccgg tgctgtatac
    41701 cggtcgcgac ggtcgcgtgg cgcacgaatg tattctcgat attcgcccgc tgaaagaaga
    41761 aaccggcatc agcgagctgg atattgccaa gcgcctgatc gactacggtt tccacgcgcc
    41821 gacgatgtcg ttcccggtgg cgggtacgct gatggttgaa ccgactgaat ctgaaagcaa
    41881 agtggaactg gatcgcttta tcgacgcgat gctggctatc cgcgcagaaa ttgaccaggt
    41941 gaaagccggt gtctggccgc tggaagataa cccgctggtg aacgcgccgc acattcagag
    42001 cgaactggtc gccgagtggg cgcatccgta cagccgtgaa gttgcggtat tcccggcagg
    42061 cgtggcagac aaatactggc cgacagtgaa acgtctggat gatgtttacg gcgaccgtaa
    42121 cctgttctgc tcctgcgtac cgattagcga ataccagtaa ttcactgatt cgactatctt
    42181 ctaaaggcgc ttcggcgcct ttttagcttg ttaataaagt gcgctttgtg tgtttcggct
    42241 gaggcgtaat ataaaatctt gcgagctcaa tatattaaaa gagtgccagg cctgatgagc
    42301 gtatcgcatc aggcacatcg gctaattaca gattctcgcc gttgctggca atcacctctt
    42361 tgtaccagtt aaagctcttc ttacgtgaac gcgacatatc gccagtaccg tcgtcatgtt
    42421 tattcacata gataaagccg tagcgtttgc tgtactgccc ggtggtgaac gatacgcagt
    42481 cgatgcagcc ccacggtgtg tagcccatca gatccacgcc atcgtaagtc accgcttttt
    42541 tcatctcttc gatatgggcg cgcaggtagt caatgcggta gtcgtcgttg atgctgccat
    42601 cttcttccac tttgtcgtaa gcgccaaaac cgttttcgac aataaacagc ggcttctggt
    42661 aacgctcata cagttcgcaa agcgcgtaac gcagacccac agggtcaatc tgccagcccc
    42721 agtcagacgc tttaacatac gggtttggta cgctgccttc aaaaccagag atagcatcgc
    42781 cggtgccgcc ttcggccttc actgcgttgg tcatgtaata gctgaaacca agataatcgc
    42841 aggtgccttc gcgcagtata tcgagatcgc catcttccat tttgatgtta aatccgcgac
    42901 gctcccactc gttcaacaca taggacgggt aatagccgcg tagctgcaca tcggtaaaga
    42961 cgtagcgttc gcgcatcgac tcctgagcga acatcacatc gtccgggtta cagcagtagg
    43021 gatagagcgg caccatcgcc agcatacagc cgactttcat ctccgggtta atgcgacgcg
    43081 cagctttcac cgccagggcg ctggcgacaa actggtgatg cagcacctga tacatcgtct
    43141 cttccgggtt ctcatgctcg gtataaacca cgccggagca gcagtaaccg aacagcggtg
    43201 cacgccagtt acgctggttg ttaatttcgt tgaaggtcat ccagtatttg actttatgct
    43261 tatagcgttc aaacaccact tccgcgaaac ggacaaagaa atcaaccact ttacggttgg
    43321 tccagctacc gtattgctgc accagatgca gcggcatttc aaagtgggag agggtgatca
    43381 ccggttcgat gttgtatttc agcagttcat cgaacatatc atcgtagaac ttcagccctt
    43441 cttcgtttgg ctgtgtttca tcgccttttg gaaaaatgcg cgtccaggcg atggatgtac
    43501 gaaaacattt gaagcccatt tcggcaaaca gcttgatgtc ttctttgtag tgaccataaa
    43561 aatcaacggc ttcatggttt ggatagtatt ttcctggcaa gacttctttg gtgatttcgc
    43621 gcggcacgcc gtgtgcgcca ccggtcagaa cgtcacaaat gctcggccct ttgccgcctt
    43681 tgttccagcc gccttcgacc tgatgagcgg caactgcgcc gccccataag aaatctttcg
    43741 gtaaggtgag ttttttcact atcataggct ccagatgagt caattcttac aggaaatagt
    43801 accatgcagc caaacgacat cacttttttt caacgtttcc aggatgacat tctggctggg
    43861 cgtaaaacca tcaccatccg cgacgagtct gaatcgcact tcaaaacggg tgatgtgctt
    43921 cgtgtcgggc gttttgaaga tgacggttat ttttgcacga ttgaagtcac cgcaacctca
    43981 accgtaacgc tggatacgct gacggaaaaa catgcagagc aggaaaatat gaccctgact
    44041 gaactgaaaa aggtcattgc cgacatctat cccggtcaga cacagtttta tgtgattgaa
    44101 tttaaatgtc tttaaattga actcacgcct gttagttgaa atttcgcatt taatttcatg
    44161 atttttaggg gattagtctc aaatcagaat ttattttagc taacaggtgt tcactggaac
    44221 tattctcagt tacgctggag aggtatacat gtgcgtgtac gtttctccgg agtaagttat
    44281 ggttcagaag cccctcatta agcagggata ttcgctggca gaggaaattg ccaacagcgt
    44341 cagtcacggc attgggttgg tgtttggtat cgttgggctg gtgttgctac tggttcaggc
    44401 ggtggatctc aatgccagcg ccacagcgat aaccagctac agcctctatg gcggcagtat
    44461 gatcctgctg ttcctcgctt cgacgctcta tcacgccatt cctcatcaac gggcaaaaat
    44521 gtggctgaag aaatttgacc attgcgctat ttacctgttg attgccggaa cctacacgcc
    44581 gtttttgctg gtggggctgg attctccgtt agcgcgcggg ttgatgattg ttatctggag
    44641 cctggcattg ctggggattc tgttcaaact gaccatcgcg caccgattca aaattttatc
    44701 tctggtgact tatctggcga tgggctggct gtcgctggtg gtaatttatg aaatggcagt
    44761 taagctcgcg gcgggcagcg ttaccttact ggcggtaggc ggcgtggttt attcgctcgg
    44821 ggtgattttc tacgtctgca aacgcattcc atacaaccat gccatctggc acggcttcgt
    44881 gctcggcggt agtgtgtgcc actttctggc gatctatttg tatattgggc aggcgtaatg
    44941 ggtatgcctt tgtaggcctg ataagacgcg ccagcgtcgc atcaggcgat aaatgcgtga
    45001 tgcgacacag agatttactc ttcgagcgaa tacggcagcg gctcgatatg caatgtattc
    45061 gcatcgtcgc gcacgcggaa gatgctgtcc ggttccatat cgttattcat cactacctgc
    45121 accacgacct gaccatcttc cagttttacc gcagccagca ccgtaccggt acgacgccag
    45181 ttctcgccca ttttcagctc taagtcttca ccagcttccg gcagtcggct ggcactacct
    45241 gtcagcaacc agagcgcgcg tttgttggca ccacggaatt ttgctcgcgc caccatctct
    45301 tgtccggtat agcagccttt cttaaagctg ataccgccca gcgcctggag gttggtcgcc
    45361 tgtgggataa actgcccgct gttggcggca tcaatcaccg ggaaaccagc ttcaatgttt
    45421 aatgccagcc actgttggct attgttcagt tccgcttcac cgcgcagttt atcggtcagc
    45481 atattggcag tagcttcatc ggttacgatc aggaaacgtt ctgccggatg ttcaaaccaa
    45541 agcagagtag tcgcaccttc tctgactacc tgcttttcct tcgaaggcag ttcactaaag
    45601 agattggcca gcgcggcgcg cgcctgaaaa ccggcaacac caagcagcac acgctcgtcg
    45661 tctggcgcga tggtcacttt agagaacacc gcatattttt tcagttcagt cagctgcgat
    45721 tcacgcacgc tgcgccgttc aatccatgca aagccatcgc cgtcgcggaa cagacgtaaa
    45781 ttgctccaca ttttaccttt ggcgtcgcaa tgggcggcca gcaggtgctg atcttctgtc
    45841 atttggctga cgtctgctgt cacctgaccc tgcatatatt tttcgctgtc cgcgccagta
    45901 atggtggcaa gcgcccagtc atcaagcgtc atcagcgtca gcggtaaacg ggcagaagcc
    45961 gtaggctgac ggggaggaaa aggtgtaaaa gccataatag agtcctgagt tgcttaacgg
    46021 cgtattaatg actaatggta aaagagctat gaggcaatgc aagcgctttc actacgcgat
    46081 ctgtgccttc tgcgaggcga cttccaaaaa aatgaagccc ttgaattgtt gtgaattaat
    46141 ttcgcgatcg cgattgtgtt tactgatatt cccgtaaaag aagtcgaaaa acacgttaca
    46201 atagcggggt actggatttt cgtgagacag gaagaagatg gacattaaca acaaagcccg
    46261 cattcattgg gcatgccgcc gtggtatgcg cgaactcgat atttcaatca tgccgttttt
    46321 cgaacatgag tacgacagct taagcgatga cgaaaaacgc atctttattc gtctgctgga
    46381 atgtgacgat ccagacctgt tcaactggct gatgaatcac ggtaaaccgg ccgatgcaga
    46441 actggaaatg atggtccgac tcatccagac acggaaccgg gaacgtggtc ctgtggcaat
    46501 ctgatttgcg cgtctcctgg cgcgcgcagt ggctttcctt gctgattcat gggctggttg
    46561 ccgctgttat tttactcatg ccctggccgc tcagttacac cccgttatgg atggtgttac
    46621 tttcgctggt ggtgtttgat tgcgttcgca gccagcggcg cattaatgct cgccaggggg
    46681 aaattcgcct gttgatggac gggcgtttgc gttggcaagg gcaggagtgg agcatcgtca
    46741 aagcgccgtg gatgattaag agcggcatga tgctgcgttt acgttctgat agcggtaaac
    46801 ggcaacattt atggctggca gccgacagca tggacgaagc cgaatggcgg gatttacggc
    46861 ggattttgtt gcaacaagag acgcaaagat aaagatgacg gcaaagcaga tgcgctaagc
    46921 cgtcagtgac atcaggcgta atgctctgcc atttcgttga ggatttgctc gcaccagccc
    46981 tgaatacgct cgtcgctaag gtcatactgg ttagtttcgt ccagcgccag acccacgaac
    47041 agttgcccgt cagcaatcac cggtttcggg ctggtaaatt catatccttc cgttggccag
    47101 tagccgacga acttcacgcc tttggtcgag agtttgtcat gcagcatccc tagcgcatcg
    47161 aggaaccact cgccgtatcc cagttgatcg ccaagcccat acagcgcaac aattttacct
    47221 tcaaggttca ggtcgtcgag ttgatcccag acggcttccc agtcttcctg gatttcgcca
    47281 aaatcccagg tcgggatacc cagaatgagc acatcgtact gctccattaa tttcggggag
    47341 tcgtccttga ggttatgtaa ggtcaccagt tctgggccga taatatcgcg gattttttct
    47401 gccgccattt cggtgtaaca ggtgctggaa ccgtaaaaaa gacccatatt cataacgtaa
    47461 gcttctcgat tctgctgtgg cttttgtggc tagtgtatca gaatcgcttt agtcacggca
    47521 caataaggca taatggatca aatgagtgac cataaggggc gcaagtgaaa caggatctgg
    47581 cacgcatcga gcagtttctt gatgctctgt ggctggaaaa aaatctggct gaaaatacgt
    47641 tgaacgctta ccgtcgcgat ctgtcaatga tggtggagtg gttgcatcac cgcgggttga
    47701 cgctggcgac ggcgcaaagt gacgatttgc aggcattact ggcagaacgg ctggagggcg
    47761 ggtataaagc caccagctca gcgcgtttgc tgagtgcagt gcgccgattg ttccagtatc
    47821 tttatcgcga aaagtttcgt gaagacgatc ccagtgcgca tctcgcttca ccgaaattgc
    47881 cccagcgttt gccaaaagat ttaagcgaag cgcaggttga acgtttatta caggcaccat
    47941 taattgatca gccactggag ctacgcgata aagccatgct tgaagtgttg tatgctaccg
    48001 ggctgcgtgt ctctgaactg gtcggactga caatgagtga tatcagcctg cgtcagggcg
    48061 tagtgcgggt cattggtaaa ggcaacaaag agcgcctggt gccgttaggt gaagaggcgg
    48121 tttactggct ggaaacctat ctggaacatg ggcgtccgtg gctgctgaat ggtgtgtcaa
    48181 ttgacgtgtt gtttcccagc cagcgtgcgc agcagatgac gcgacagacc ttctggcacc
    48241 gtattaaaca ttatgccgtg ctggcgggta tcgacagcga aaagctgtca ccgcatgtgt
    48301 tgcgtcacgc ttttgccact catttattaa atcatggtgc ggatttacgc gtggtgcaga
    48361 tgctactagg ccacagcgat ctctccacca cgcaaattta tacgcatgtc gctaccgagc
    48421 gtctgcggca acttcatcaa cagcatcacc cgcgggcgtg atgtctgaaa agaacgggaa
    48481 gatttatgaa gaaaggtttt atgttgttta ctttgttagc ggcgttttca ggctttgctc
    48541 aggctgatga cgcggcaatt caacaaacgt tagccaaaat gggcatcaaa agcagcgata
    48601 ttcagcccgc gcccgtagct ggcatgaaga cagttctgac taacagcggt gtgttgtaca
    48661 tcaccgatga tggtaaacat atcattcagg ggccaatgta tgacgttagc ggcacggctc
    48721 cggtcaatgt caccaataag atgctgttaa agcagttgaa tgcgcttgaa aaagagatga
    48781 tcgtttataa agcgccgcag gaaaaacacg tcatcaccgt gtttactgat attacctgtg
    48841 gttactgcca caaactgcat gagcaaatgg cagactacaa cgcgctgggg atcaccgtgc
    48901 gttatcttgc tttcccgcgc caggggctgg atagcgatgc tgagaaagaa atgaaagcta
    48961 tctggtgtgc gaaagacaaa aacaaagcgt ttgatgatgt gatggcaggt aaaagcgtcg
    49021 caccagccag ttgcgacgtg gatattgccg accattacgc acttggcgtc cagcttggcg
    49081 ttagcggtac tccggcagtt gtgctgagca atggcacact tgttccgggt taccagccgc
    49141 cgaaagagat gaaagaattc ctcgacgaac accaaaaaat gaccagcggt aaataattcg
    49201 cgtgaaacaa cagatacaac ttcgtcgccg tgaagtcgat gaaacggcag acttgcccgc
    49261 tgaattgcct cccttgctgc gccgtttata cgccagccga ggagtacgca gtgcgcaaga
    49321 actggaacgc agtgttaaag gtatgttgcc ctggcagcaa ctgagcggcg tcgaaaaggc
    49381 cgttgagatc ctttacaacg cttttcgcga aggaacgcgt attattgtgg tcggtgattt
    49441 cgacgccgac ggcgcgacca gcacggctct aagcgtgctg gcgatgcgct cgcttggttg
    49501 cagcaatatc gactacctgg taccaaaccg tttcgaagac ggttacggct taagcccgga
    49561 agtggtcgat caggcccatg cccgtggcgc gcagttaatt gtcacggtgg ataacggtat
    49621 ttcctcccat gcgggggttg agcacgctcg ctcgttgggc atcccggtta ttgttaccga
    49681 tcaccatttg ccaggcgaca cattacccgc agcggaagcg atcattaacc ctaacttgcg
    49741 cgactgtaat ttcccgtcga aatcactggc aggcgtgggt gtggcgtttt atctgatgct
    49801 ggcgctgcgc acctttttgc gcgatcaggg ctggtttgat gagcgtaaca tcgcaattcc
    49861 taacctggca gaactgctgg atctggtcgc gctggggaca gtggcggacg tcgtgccgct
    49921 ggacgctaat aatcgcattc tgacctggca ggggatgagt cgcatccgag ccggaaagtg
    49981 ccgtccgggg attaaagcgc tgcttgaagt ggcaaaccgt gatgcacaaa aactcgccgc
    50041 cagcgattta ggttttgcgc tggggccacg tctcaatgct gccggacgac tggacgatat
    50101 gtccgtcggt gtggcgctct tgctgtgcga caacatcggc gaagcgcgcg tgctggcaaa
    50161 tgaactcgat gcgctaaacc agacgcgaaa agagatcgaa caaggaatgc aggttgaagc
    50221 cctgaccctg tgcgagaaac tggagcgcag ccgcgacacg ctacccggcg ggctggcaat
    50281 gtatcacccc gaatggcatc agggcgttgt cggtattctg gcttcgcgca tcaaagagcg
    50341 ttttcatcgt ccggttatcg cctttgcgcc agcaggtgac ggtacgctga aaggttccgg
    50401 tcgctccatt caggggctgc atatgcgtga tgcgctggag cgattagaca cactctaccc
    50461 tggcatgatg ctgaagtttg gcggtcatgc gatggcggcg ggtttgtcgc tggaagagga
    50521 taaattcgaa ctctttcaac aacggtttgg cgaactggtt actgagtggc tggacccttc
    50581 gctattgcaa ggcgaagtgg tgtcagacgg tccgttaagc ccggccgaaa tgaccatgga
    50641 agtggcgcag ctgctgcgcg atgctggccc gtgggggcag atgttcccgg agccgctgtt
    50701 tgacggtcat ttccgtctgc tgcaacagcg gctggtgggc gaacgtcatt tgaaagtcat
    50761 ggtcgaaccg gtcggcggcg gtccgctgct ggatggtatt gcttttaatg tcgataccgc
    50821 cctctggccg gataacggcg tgcgcgaagt gcaactggct tacaagctcg atatcaacga
    50881 gtttcgcggc aaccgcagcc tgcaaattat catcgacaat atctggccaa tttagcgtca
    50941 tcttctctat aaaaaagagc gtggattggg tacaatcccg ctcttatcac cgcattttga
    51001 ctagctcaat aaaagaaatc agaccatgtt tgaaattaat ccggtaaata atcgcattca
    51061 ggacctcacg gaacgctccg acgttcttag ggggtatctt tgactacgac gccaagaaag
    51121 agcgtctgga agaagtaaac gccgagctgg aacagccgga tgtctggaac gaacccgaac
    51181 gcgcacaggc gctgggtaaa gagcgttcct ccctcgaagc cgttgtcgac accctcgacc
    51241 aaatgaaaca gggactggaa gatgtttctg gtctgctgga actggctgta gaagctgacg
    51301 acgaagaaac ctttaacgaa gccgttgctg aactcgacgc cctggaagaa aaactggcgc
    51361 agcttgagtt ccgccgtatg ttctctggcg aatatgacag cgccgactgc tacctcgata
    51421 ttcaggcggg gtctggcggt acggaagcac aggactgggc gagcatgctt gagcgtatgt
    51481 atctgcgctg ggcagaatcg cgtggtttca aaactgaaat catcgaagag tcggaaggtg
    51541 aagtggcggg tattaaatcc gtgactatca aaatctccgg cgattacgct tacggctggc
    51601 tgcgtacaga aaccggcgtt caccgcctgg tgcgtaaaag cccgtttgac tccggcggtc
    51661 gtcgccatac gtcgttcagc tccgcgtttg tttacccgga agtcgatgac gatattgata
    51721 tcgaaatcaa cccggcggat ctgcgcattg acgtttatcg cgcgtccggc gcgggcggtc
    51781 agcacgttaa ccgtaccgaa tctgcggtgc gtattactca catcccaacc gggatcgtga
    51841 cccagtgcca gaacgaccgt tcccagcaca agaacaaaga ccaggccatg aagcagatga
    51901 aagcgaagct ttatgaactg gagatgcaga agaaaaatgc cgagaaacag gcgatggaag
    51961 ataacaaatc cgatatcggc tggggcagcc agattcgttc ttatgtcctt gatgactccc
    52021 gcattaaaga tctgcgcact ggggtagaaa cccgcaacac gcaggccgtg ctggacggca
    52081 gcctggatca atttatcgaa gcaagtttga aagcagggtt atgaggaacc aacatgtctg
    52141 aacaacacgc acagggcgct gacgcggtag tcgatcttaa caatgaactg aaaacgcgtc
    52201 gtgagaagct ggcgaacctg cgcgagcagg ggattgcctt cccgaacgat ttccgtcgcg
    52261 atcatacctc tgaccaattg cacgcagaat tcggcggtaa agagaacgaa gaactggaag
    52321 cgctgaatat cgaagtcgcc gttgctggcc gcatgatgac ccgccgtatt atgggtaaag
    52381 cgtctttcgt taccctgcag gacgttggcg gtcgcattca gctgtacgtt gcccgtgacg
    52441 atctgccgga aggcgtttac aacgagcagt tcaaaaaatg ggacctcggc gacatcctcg
    52501 gcgcgaaagg taaactgttc aaaaccaaaa ccggcgaact gtctatccac tgtaccgagc
    52561 tgcgtctact gaccaaagca ctgcgtccgc tgccggataa attccacggc ttgcaggatc
    52621 aggaagcgcg ctatcgtcag cgttatctgg atctcatctc taacgatgaa tcccgtaaca
    52681 cctttaaagt gcgctcgcag atcctctctg gtattcgcca gttcatggtg aaccgcggct
    52741 ttatggaagt tgaaacgccg atgatgcagg tgatccctgg cggtgccgct gcgcgtccgt
    52801 ttatcaccca ccataacgcg ctggatctcg acatgtacct gcgtatcgcg ccggaactgt
    52861 acctcaagcg tctggtggtc ggtggcttcg agcgtgtatt cgaaatcaac cgtaacttcc
    52921 gtaacgaagg tatttccgta cgtcataacc cagagttcac catgatggaa ctctacatgg
    52981 cttacgcaga ttacaaagat ctgatcgagc tgaccgaatc gctgttccgt actctggcac
    53041 aggatattct cggtaagacg gaagtgacct atggcgacgt gacgctggac ttcggtaaac
    53101 cgttcgaaaa actgaccatg cgtgaagcga tcaagaaata tcgcccggaa accgacatgg
    53161 cggatctgga caacttcgac tctgcgaaag cgattgctga atctatcggc atccacgttg
    53221 agaagagctg gggtctgggc cgtatcgtta ccgagatctt cgaagaagtg gcagaagcac
    53281 atctgattca gccgaccttc attactgaat atccggcaga agtttctccg ctagcgcgtc
    53341 gtaacgacgt taacccggaa atcacagacc gctttgagtt cttcattggt ggtcgtgaaa
    53401 tcggtaacgg ctttagcgag ctgaatgacg cggaagatca ggcgcagcgc ttcctggatc
    53461 aggttgccgc gaaagacgca ggtgacgacg aagcgatgtt ctacgatgaa gattacgtta
    53521 ccgcactgga acatggctta ccgccgacag caggtctggg aattggtatc gaccgtatgg
    53581 taatgctgtt caccaacagc cataccatcc gcgacgttat tctgttcccg gcgatgcgtc
    53641 cggtgaaata agcattacgt tatgctcaca accccggcaa atgtcggggt tttttattta
    53701 agctgggtaa atgcagataa tcgtattctg gcttcgcgat ttgtcgcctg catcaccatc
    53761 cacggactga acgcccacgg cgtggcatca ataccgcgta atacatcagc taaatcacac
    53821 cattgataat ccatcacttc atcatcgttg atctgtaacg cactggtggt gcgtgcggca
    53881 aataccggac acacttcatt ttccacaatg ccattcggat cggtggcgcg atagcgaaag
    53941 tcaggataga tagattcagg aggcgtaatt tccacgccaa gctcataacg gcaacggcgg
    54001 atcaccgcgt cttcgttgct ttctcccagt tgtgggtgcc cacaaaccga gttagtccac
    54061 acgccaggcc atgctttttt gctcagtgcg cggcgggtaa ctaataattg tcctttggca
    54121 ttaaacagcc aactggagaa cgcgagatgt aagcgggtgt ctgccgtgtg tgcggcatac
    54181 ttttccagcg tacccgtggg aactccctgt gcattcaata aaatgacgtg ttccgtttgc
    54241 ataatttctc acatgtaatt ctgatcactc atcattatag ataattgaag agtgaaatga
    54301 ttaaccaagt gagcgtttat cgacaaccgc ccgttttgag cggatgtcga caggtaaaaa
    54361 ccatttaaaa aagtagcatc atatatttga agcgactttc aatggccatt gcaacacagt
    54421 cagaaatgac aggaatagag agagtaaaaa gcaacagcag actgaatata gcgcgcatta
    54481 aaaaattcat attattctcc atgctcattt ttcttcagct tgcgcatcag aagccacata
    54541 cgtactgtcc tgacttttaa atctttatca gagacggatt ccttcaccag gtcagcatga
    54601 tgctggtagc cattaaagaa gacattcagc acaacggcgc taagggttgc gagcataatg
    54661 ccgctatgca gcagcggttg cagtacggcc ggtaacttag aaaagaaatc gtgagagagc
    54721 gtcggagtca tgccaacgcc gagactgatc gccacaatat agaggttgta acggttggtg
    54781 gtgtagttac agcgcgacag aattcgaatc cctgtcgcca gtaccatgcc gaacatcacc
    54841 agcccagcgc cgcccagcac aaattgcgga atggaggcta ccagcaccgc cattttgggc
    54901 accatgccaa acaggattaa tataattccc gaagaaatac acacccagcg gctatgaacg
    54961 cgcgtcacgc taaccaggcc aacgttttga gaaaatgacg tgtgggggaa gctgttaaac
    55021 gtgccaccaa tcattgtccc tacgccatcg acacgcagcc cgcgaataat atcgtgcgaa
    55081 gagagtttac gaccgactat ttcacccagt gccaggaaca tccccatcga ctcgataaac
    55141 acgatgatta acacggcagt catggtcaga atggaaacgg gatcgaaaat cggcatacca
    55201 aacgacatcg gcgtaacaat cgcaaaccac gaggcatcat gtagcccgga taaattgact
    55261 tcattcatca tccacgaaag taaaaagcca aatacaatcc ccagtaatac ggcaacgttg
    55321 gacataaatc ctttcgcata gcgagtaatg agcaagataa aaattaagac ggcaaaggag
    55381 atacctaaat aaacgggatt accatattgc ggattccctt tacctccggc ggcccagtca
    55441 atacccacct gaatgatgct aagcccgata gaagtaataa ccacaccggt aaccagtggc
    55501 ggaaataaag gcatcaagcg accgataagt ggcgctaata atgtggtgat aaaacccgcg
    55561 gcgatagtgg caccaaatat ccccagcagg ccgatatccg ggttcatccc aatggctatc
    55621 attggtgtta cagcagcaaa ggtcaccgac ataatcaccg gcaggcggat ccccataaag
    55681 cggccgatac cgatacattg caataatgtg acgatcccgc agcaaaagag atccgagcta
    55741 atgagcatcg caatagcatc ttttgagagg cccagtcggt caccaatcat aagaggaaca
    55801 gcgactgcac ctgcgtacat gacaaggacg tgttgcagac cgagtatgat cagctttcct
    55861 ggtgataata tgcggtcaac ctcatcagtt gggcggtctt gcccagaaga tgagggaagt
    55921 tgggaatcta tggcgctcat cagtgtttct cctttcgccc gccagagtaa aaagcaaccg
    55981 tttacgttaa tgaaactgtc taaacacatg ctcgtggagt gccagttttt gcgaggaagg
    56041 ggagttagag acaggaattg cggaggcaat cacataatcg tggttaaacc acgaaaatga
    56101 gaagagaacc tgacaatgga attatctgta attttctcat attgatattt cattaatcat
    56161 tctggcatgt cgcactctcg catttaatcg tttttatctg gatagcgctc ttttgatcgg
    56221 caatgttgaa ataatgaaag cggcatagat tgaggggcaa taatttgaac tcagtcgcaa
    56281 aattataatt aaaacggtca tgctacctaa tatatgcccg tttattgcat cgcgaaattt
    56341 gagaagacat aactcattaa atgaaaggga tgcaaatgaa taagtttatc gctgctgaag
    56401 ctgcggaatg tataggctgt catgcttgtg aaattgcctg tgcggtggca cacaatcaag
    56461 aaaactggcc gctgagtcac agtgactttc gaccacgtat ccacgttgta gggaaaggcc
    56521 tggctgcgaa tccggtggcc tgccatcact gcaacaatgc cccttgcgtt acggcttgtc
    56581 cggttaatgc tctgactttc cagtccgata gcgtacaact ggacgagcaa aaatgtattg
    56641 gttgtaaaag atgcgcaatc gcttgcccct ttggcgtcgt tgagatggtc gatacgattg
    56701 cgcagaaatg cgacctttgt aaccagcgca gttccggcac gcaagcctgt attgaagtct
    56761 gcccaacaca ggcgttacgg ctgatggacg ataaagggtt acagcagata aaggtggccc
    56821 gccagcgcaa aacggcagca ggaaaagcgt catcagacgc tcagccatct cgcagtgcag
    56881 cgttgctccc cgttaactcg cgtaaaggcg cagataaaat ttcagcaagt gaacggaaaa
    56941 cccactttgg cgaaatctat tgcgggctgg atccactgca agcgacttat gagagtgacc
    57001 gctgtgttta ttgtgccgaa aaagctaact gcaactggca ttgcccgctg cataacgcta
    57061 ttccggatta catccgtctg gtacaggaag gaaagattat tgaagcggca gaactttgcc
    57121 accagaccag ttccttacca gaaatttgcg gcagggtatg tccacaggac cgtctttgtg
    57181 aaggcgcatg tactttgaaa gatcactcgg gcgcagtctc tatcggtaat ctggaacgct
    57241 acatcaccga taccgcgctg gcgatgggct ggcgtcccga tgtcagcaaa gttgttcccc
    57301 gtagcgaaaa agtggcggtg attggcgctg ggccagcagg gttagggtgt gctgatattc
    57361 tggcgcgcgc gggagttcag gttgatgtct ttgatcgcca tccagaaatt ggcggtatgc
    57421 tgacttttgg cattcctcct ttcaaactcg ataaaacggt attaagccag cggcgagaga
    57481 tattcaccgc aatgggaatc gactttcatc ttaactgtga aattggccgc gatattacct
    57541 ttagcgattt aacttctgaa tatgatgcag ttttcatcgg cgtggggact tacgggatga
    57601 tgcgagcaga tctgccgcat gaagatgcgc ccggtgtcat tcaggctcta ccgttcctga
    57661 ctgcccatac ccgccagctc atgggattgc cggagtctga agagtatccg ctgacggacg
    57721 tggaaggtaa gcgagtcgtg gtattgggcg gtggcgatac gacaatggat tgtttgcgga
    57781 cttccatccg cctcaatgcc gccagcgtga cctgcgcgta tcgtcgtgat gaagtcagta
    57841 tgccgggctc gcgcaaagag gtggttaatg cgcgcgagga aggtgtcgag tttcagttca
    57901 atgttcaacc gcaatatatc gcttgtgacg aagatgggcg tttaactgcg gtgggcctga
    57961 ttcgtaccgc catgggtgag ccggggccgg atggtcgccg tcgtcctcgt ccggtagcgg
    58021 gttcagagtt tgaattgccc gccgatgttc tgattatggc ctttggtttc caggcgcatg
    58081 ccatgccgtg gttgcagggc agcggaatta aactcgataa atggggcctg attcaaaccg
    58141 gtgacgtcgg gtatttacct acccagacgc atctgaaaaa agtctttgct ggtggtgatg
    58201 cagttcatgg cgcggatctg gttgtcactg caatggccgc aggaaggcag gcggcgcgcg
    58261 atatgttaac tctgtttgat acgaaggcat cgtgatgaaa tcgttaatta tcgttaatcc
    58321 ggctgactgt attggctgcc gtacttgtga agtagcctgt gtggtcgctc acccttcaga
    58381 acaggagttg aatgccgatg tctttttacc ccggctgaag gtacagcgac tggacagcat
    58441 cagtgcgccg gtgatgtgcc atcagtgtga aaacgcccct tgtgttggcg cttgccccgt
    58501 gggggcgctg acgatgggcg agcaggtggt gcagaccaat tctgcccgtt gtattggctg
    58561 tcagagttgc gtcagtgcgt gcccgttggg aatgatcacc attcagtcgt tgccggggga
    58621 tacccgacaa caaattgtga aatgcgatct ctgtgaacag cgggaagagg ggccagcctg
    58681 tgttgaatct tgcccgacac aggcgctgca gttgcttact gaaagagaac tcaggcgagt
    58741 ccgccagcag cgtattgttg ccagcggtga gaatcctctg tgaaagaaat ctccgcgctg
    58801 aaaggcgcgg agaatctatt agattgccca accacccgcg taaaatgcga ccagtgcggc
    58861 agtaatgatc accgtaccaa tattcaactt ttgccattcg cgagcaaaga cacggcctac
    58921 gaccagtgtc acaaagccca gcataatacc ggtaacgata ttacaagtca gaacgatgaa
    58981 cacggcacac accaggccag ccattgcgtc aataaaatcg ttgaaatcca gcttcgagac
    59041 gttacttaac atcagcaaac ccacgtacat cagagcgggt gcagtggcgt aaccagggat
    59101 caaaaatgac agcggtgata agaacagaat cagcaggaat aacgccccca ctacggttgc
    59161 ggtcaaccct gttttacccc cggcggcggt tcctgccgct gattcgatat aaaccgctgc
    59221 gggcgctgcg cccaccagac cggagaatat tgaacttact gagtcactgg tcagggcttt
    59281 gccgccattg atgatctggt tgtctttatc caacaaattc gcctgaccgg cgacggcacg
    59341 gatggtgcca gtggcgtcga acactgcggt catcaccaat gccagtacac tcggaagtac
    59401 ggttggctgg agtgcgccca taatatcgag gctgaaaatc agagacttac catcttcgcc
    59461 agtcagactt ggcatcgcca ccagaccgtg gtatttcacc gctgggtcaa agattaagcc
    59521 gataatcgaa attgcgataa tcaccaacaa gatcccgccg ggtacgcggc acttctccag
    59581 accgaagatg accgccagcc ccagcaagct catcatcacc gggaaggagg taaacgcacc
    59641 gagcgccact ggcaagcctt caatcgggtt tttgataacc atacccacac cgttagcagc
    59701 aatcagcagc agaaacaggc cgataccgat acctgtaccg tgagcgatac ccatcggtaa
    59761 attacgtaag atccaggtac gcacaccggt tacggaaata gcggtgaaga tgacgcccat
    59821 cagaaagacc gcgcccagtg cgacaggaac gctaatttgt tgcccgagta ccagactgaa
    59881 tgcggtaaat gccgtcaagg aaatcgcaca accaatcgcc attggcaaat tggcccacaa
    59941 ccccatcagc aacgagccga agcccgcgac cagacaggtg gcaacaaaca cagctgcggg
    60001 aggaaaacct gctttgccca gcattcccgg aacgacgata acggaataaa ccatggccag
    60061 aaaggtcgtt aagccagcca gtacttcctg acgaacggta ctgccacgag cggtaatttt
    60121 aaaataatta tccagcgcgc cctgtggctt tggtgcgtcc ggtgtttgta ggatgtctcc
    60181 agacataatg atgtcctcac gaggatgtta aagttttatt attagttgcg ttcgtacacc
    60241 agacgaccat caacgtaggt gcggtagatc gaacggtcat cgcccaacgt catcatcacg
    60301 aacaatttgt cgactaaaga aacagagttg tcatagcgca gctgctgtag cggagtggcg
    60361 gtgggttcca tcaccacgaa atcagcctct ttgccaggta aaaagttgcc aatcaaatcg
    60421 tcaaggccca gagatttcgc tccgccgagc gtggccaggt aaaacgcttc atatgccgag
    60481 aggcgatagc cttgtaattg caatactttg taggcttcgt tcagcgtttg cagcatgttg
    60541 aaagtggttc cggcaccgat atccgttccc atgcccactt taactttctt ctgccatgct
    60601 tttttcaagt tgaataaccc gctgccgagg taaaggttgg aggtcggaca gaaagcaatg
    60661 ctggatttgg tttcgctgag acgatcccac tctttttctt cgagatggac gcagtgagca
    60721 aagacacagt ttttaccggt caggccgtac tgatggtaaa catccagata accatcatgg
    60781 tcaggataaa gcgatttcac ccaggcaatt tcatctttgt tttcacagag atgggtatgt
    60841 acccacgtat ccggatattc ctctttcagg cgttgcgcca tcgccatctg ttcaggagat
    60901 gaggtagggg cgaatcgtgg cgtaatcgca tacaacagac gaccattttt gtgccagcgt
    60961 tcgatcagtt ctttgctttg gtgatagctg ctttcggcag tgtcgagcag ataatccggt
    61021 gcgttgcggt ccatcatcac cttaccggca atcatacgca tattgatatg actggcggct
    61081 tcaaacagcg catcaacaga ttgcggatga acagtgccaa acaccagcgc cgtggtggtt
    61141 ccgttacgta aaagctgctt gatgaagaac gccgacattt cgcgggcgta ctctaaatcc
    61201 tcataacgac gttcagtagg gaaggtgtgt ttattcaacc actccagcaa ttgctcacca
    61261 taggccccca ccatttcact ttgcggataa tggatatgtg tatcgacaaa gcccggtact
    61321 atcagtttgc cgcgatagtc gcgcacgcga atggtgtcag gaatttgatg ctttccgttt
    61381 tcccattcgc caaaccattc cacttttccc tgtttaatga gtaataaacc atcctcaata
    61441 aaccgcagcg cagaggcaat ctcttccggg ttatcgaccg tacgggtgac atcaataaaa
    61501 ctgcctcgta ccgcttttaa cgtgtgtttt cctgacatca ttgactcctt tactgttaat
    61561 ccatctcttc cgctgaggta ataccaggca gaacgttttc ctgtcggtag ccaccaggga
    61621 ggataatatt taataaaatc gcagttaacc cgccagcaca aatagggttt tcaactaata
    61681 cataaataga ggctggcaat attttaaaaa tttcgggatc gtaggagacg ccaagcccta
    61741 aacctaaaga agtggcgaca ataagtgttt cacgccgctt taatccgttg gtgatgatga
    61801 tgcgaatccc agcgatggca atcatggaaa acatcaacgt cattgcgcct cccagaactg
    61861 ccgagggaat ggtcgtgaag aagccgccaa tcatcggaaa taagccgagg ataaccagca
    61921 ttaccgcgat ggttcgcccg acataacgtg aagcgacgcc agtcatctga ataaccccat
    61981 tattttgcgc aaacgtggtt aatggtaatg aaccaacagc ggaggcgata acagaaacca
    62041 gaccatctgc cagcacgccg cctttcagtc gggactgata ctcttccccc tgaatggggc
    62101 ggcgggaaac cattgccgtg gcggtgatat cgccgactgc ttccagcacg ctaagcagat
    62161 aaatcgtgcc aaccaccagg aactgatgga agctaaaact aaagccgtat ttgaacggat
    62221 gtgggatggt gattaacggc aaattgcgca tactgctgaa atccaccatg cccaggcata
    62281 acgacgcgat ataaccgacg catagcccaa tggcgatccc tcccatgcgt agcaacggac
    62341 tgcgacagca gttaaagccg atcaccacaa tcaaaaccaa caaaccaacg ccgagatgtt
    62401 cgtaattgcc gaacgtaccg ctgcttttgg cagcaaaccc accgccaaaa tcgataatgc
    62461 cgactttaat caggcttaag ccgatcatca gtaccacaat accgctgacg gtaggcgtaa
    62521 taacccgacg taaatagggc aagataaatg aagatccgac aaccagaaat gcgccaacga
    62581 aggagacgcc gagaagcgac gacatgatta actcttcgtg aaaaccgtcg cttttcatgc
    62641 tgctgcccag cgcaatcatg accgtaacaa atgaaaaatt gactgactga attgagagta
    62701 ggccagaacc gacgatgccg tagcggttta cttgtaacca ggtaccaata ccagaggcga
    62761 tcatcgccat agaaacaaga taggcggttg tttcagcgga aagctgtaag gccgcaccca
    62821 cgattaacgc gggggttacc atcggaacga aaattgccaa cagatgggta atggcaccaa
    62881 cgagagcctg atgaaaggga gggcgatcct ccagttcaaa tataaggtca gaacctgcat
    62941 ggtt
//
LOCUS       DAPGOF010000003        61934 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.003, whole genome shotgun sequence.
ACCESSION   DAPGOF010000003 DAPGOF010000000
VERSION     DAPGOF010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 61934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61934
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.003"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(203..745)
                     /locus_tag="RHV13_000123"
     CDS             complement(203..745)
                     /locus_tag="RHV13_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV1239690.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(768..1793)
                     /gene="nagZ"
                     /locus_tag="RHV13_000124"
     CDS             complement(768..1793)
                     /gene="nagZ"
                     /locus_tag="RHV13_000124"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HDV1239691.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPEKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSTLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(1804..2628)
                     /gene="thiK"
                     /locus_tag="RHV13_000125"
     CDS             complement(1804..2628)
                     /gene="thiK"
                     /locus_tag="RHV13_000125"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HDV1239692.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(2609..3250)
                     /gene="lpoB"
                     /locus_tag="RHV13_000126"
     CDS             complement(2609..3250)
                     /gene="lpoB"
                     /locus_tag="RHV13_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HDV1239693.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(3264..3641)
                     /locus_tag="RHV13_000127"
     CDS             complement(3264..3641)
                     /locus_tag="RHV13_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HDV1239694.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(3644..4003)
                     /gene="hinT"
                     /locus_tag="RHV13_000128"
     CDS             complement(3644..4003)
                     /gene="hinT"
                     /locus_tag="RHV13_000128"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HDV1239695.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            4337..6526
                     /gene="fhuE"
                     /locus_tag="RHV13_000129"
     CDS             4337..6526
                     /gene="fhuE"
                     /locus_tag="RHV13_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HDV1239696.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFVSRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(6586..8019)
                     /gene="ptsG"
                     /locus_tag="RHV13_000130"
     CDS             complement(6586..8019)
                     /gene="ptsG"
                     /locus_tag="RHV13_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HDV1239697.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(8314..9111)
                     /locus_tag="RHV13_000131"
     CDS             complement(8314..9111)
                     /locus_tag="RHV13_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDV1239698.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(9122..10126)
                     /gene="holB"
                     /locus_tag="RHV13_000132"
     CDS             complement(9122..10126)
                     /gene="holB"
                     /locus_tag="RHV13_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDV1239699.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQVALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(10123..10764)
                     /locus_tag="RHV13_000133"
     CDS             complement(10123..10764)
                     /locus_tag="RHV13_000133"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDV1239700.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(10754..11776)
                     /gene="yceG"
                     /locus_tag="RHV13_000134"
     CDS             complement(10754..11776)
                     /gene="yceG"
                     /locus_tag="RHV13_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HDV1239701.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGIWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYITVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(11779..12588)
                     /gene="pabC"
                     /locus_tag="RHV13_000135"
     CDS             complement(11779..12588)
                     /gene="pabC"
                     /locus_tag="RHV13_000135"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HDV1239702.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMSVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(12708..13949)
                     /gene="fabF"
                     /locus_tag="RHV13_000136"
     CDS             complement(12708..13949)
                     /gene="fabF"
                     /locus_tag="RHV13_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDV1239703.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(14037..14273)
                     /gene="acpP"
                     /locus_tag="RHV13_000137"
     CDS             complement(14037..14273)
                     /gene="acpP"
                     /locus_tag="RHV13_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDV1239704.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(14484..15218)
                     /gene="fabG"
                     /locus_tag="RHV13_000138"
     CDS             complement(14484..15218)
                     /gene="fabG"
                     /locus_tag="RHV13_000138"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HDV1239705.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(15231..16160)
                     /gene="fabD"
                     /locus_tag="RHV13_000139"
     CDS             complement(15231..16160)
                     /gene="fabD"
                     /locus_tag="RHV13_000139"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDV1239706.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPALMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(16176..17129)
                     /gene="fabH"
                     /locus_tag="RHV13_000140"
     CDS             complement(16176..17129)
                     /gene="fabH"
                     /locus_tag="RHV13_000140"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HDV1239707.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(17197..18267)
                     /gene="plsX"
                     /locus_tag="RHV13_000141"
     CDS             complement(17197..18267)
                     /gene="plsX"
                     /locus_tag="RHV13_000141"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDV1239708.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(18348..18521)
                     /gene="rpmF"
                     /locus_tag="RHV13_000142"
     CDS             complement(18348..18521)
                     /gene="rpmF"
                     /locus_tag="RHV13_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDV1239709.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(18573..19094)
                     /gene="yceD"
                     /locus_tag="RHV13_000143"
     CDS             complement(18573..19094)
                     /gene="yceD"
                     /locus_tag="RHV13_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HDV1239710.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            19293..19877
                     /gene="yceF"
                     /locus_tag="RHV13_000144"
     CDS             19293..19877
                     /gene="yceF"
                     /locus_tag="RHV13_000144"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HDV1239711.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPHSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(19989..20948)
                     /gene="rluC"
                     /locus_tag="RHV13_000145"
     CDS             complement(19989..20948)
                     /gene="rluC"
                     /locus_tag="RHV13_000145"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HDV1239712.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDDGLKRCLQKLRNAR"
     gene            21521..24706
                     /gene="rne"
                     /locus_tag="RHV13_000146"
     CDS             21521..24706
                     /gene="rne"
                     /locus_tag="RHV13_000146"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HDV1239713.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKSAPATPAAPA
                     QPGLLSRFFGALKALFSGGEETKPSEQAAPKVEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTADEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETVAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTELPVAAAVEPVVSAPVVEEVAEVVEAPVQIAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVGAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(24902..25855)
                     /gene="flgL"
                     /locus_tag="RHV13_000147"
     CDS             complement(24902..25855)
                     /gene="flgL"
                     /locus_tag="RHV13_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HDV1239714.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEEKGKYVGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(25867..27510)
                     /gene="flgK"
                     /locus_tag="RHV13_000148"
     CDS             complement(25867..27510)
                     /gene="flgK"
                     /locus_tag="RHV13_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HDV1239715.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLAVAFAEAFNSQHKAGFDANGDAGEDFFAIGKPA
                     VLQDTKNKGNVAIGATVTDASAVLATDYKISFDNNQCQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(27576..28517)
                     /gene="flgJ"
                     /locus_tag="RHV13_000149"
     CDS             complement(27576..28517)
                     /gene="flgJ"
                     /locus_tag="RHV13_000149"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HDV1239716.1"
                     /translation="MISDSKLQASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEEPTSAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(28517..29614)
                     /gene="flgI"
                     /locus_tag="RHV13_000150"
     CDS             complement(28517..29614)
                     /gene="flgI"
                     /locus_tag="RHV13_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HDV1239717.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(29626..30324)
                     /gene="flgH"
                     /locus_tag="RHV13_000151"
     CDS             complement(29626..30324)
                     /gene="flgH"
                     /locus_tag="RHV13_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HDV1239718.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(30377..31159)
                     /gene="flgG"
                     /locus_tag="RHV13_000152"
     CDS             complement(30377..31159)
                     /gene="flgG"
                     /locus_tag="RHV13_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HDV1239719.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(31331..32086)
                     /gene="flgF"
                     /locus_tag="RHV13_000153"
     CDS             complement(31331..32086)
                     /gene="flgF"
                     /locus_tag="RHV13_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HDV1239720.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(32106..33311)
                     /gene="flgE"
                     /locus_tag="RHV13_000154"
     CDS             complement(32106..33311)
                     /gene="flgE"
                     /locus_tag="RHV13_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HDV1239721.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVNAFDASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(33336..34031)
                     /gene="flgD"
                     /locus_tag="RHV13_000155"
     CDS             complement(33336..34031)
                     /gene="flgD"
                     /locus_tag="RHV13_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HDV1239722.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNNGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(34043..34447)
                     /gene="flgC"
                     /locus_tag="RHV13_000156"
     CDS             complement(34043..34447)
                     /gene="flgC"
                     /locus_tag="RHV13_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HDV1239723.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(34451..34867)
                     /gene="flgB"
                     /locus_tag="RHV13_000157"
     CDS             complement(34451..34867)
                     /gene="flgB"
                     /locus_tag="RHV13_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HDV1239724.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            35023..35682
                     /gene="flgA"
                     /locus_tag="RHV13_000158"
     CDS             35023..35682
                     /gene="flgA"
                     /locus_tag="RHV13_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HDV1239725.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLHTFFSAQLVGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVTGQVVSGVVDADGNILIN
                     L"
     gene            35758..36051
                     /gene="flgM"
                     /locus_tag="RHV13_000159"
     CDS             35758..36051
                     /gene="flgM"
                     /locus_tag="RHV13_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HDV1239726.1"
                     /translation="MSIDRTSPLNPVSTVQPRETTDAPVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMNTGKIADALINEAQQDLQSN"
     gene            36056..36472
                     /gene="flgN"
                     /locus_tag="RHV13_000160"
     CDS             36056..36472
                     /gene="flgN"
                     /locus_tag="RHV13_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HDV1239727.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQVIAVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(36512..38047)
                     /gene="murJ"
                     /locus_tag="RHV13_000161"
     CDS             complement(36512..38047)
                     /gene="murJ"
                     /locus_tag="RHV13_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDV1239728.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(38157..39080)
                     /locus_tag="RHV13_000162"
     CDS             complement(38157..39080)
                     /locus_tag="RHV13_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDV1239729.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPNDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITEMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(39082..39729)
                     /locus_tag="RHV13_000163"
     CDS             complement(39082..39729)
                     /locus_tag="RHV13_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HDV1239730.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVELTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPSVTDMSSAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(39740..40324)
                     /gene="rimJ"
                     /locus_tag="RHV13_000164"
     CDS             complement(39740..40324)
                     /gene="rimJ"
                     /locus_tag="RHV13_000164"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HDV1239731.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            40560..41768
                     /gene="mdtH"
                     /locus_tag="RHV13_000165"
     CDS             40560..41768
                     /gene="mdtH"
                     /locus_tag="RHV13_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HDV1239732.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     TARRLLERDA"
     gene            41832..42479
                     /gene="grxB"
                     /locus_tag="RHV13_000166"
     CDS             41832..42479
                     /gene="grxB"
                     /locus_tag="RHV13_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HDV1239733.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNVSDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            42613..43173
                     /locus_tag="RHV13_000167"
     CDS             42613..43173
                     /locus_tag="RHV13_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDV1239734.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            43279..44325
                     /gene="pyrC"
                     /locus_tag="RHV13_000168"
     CDS             43279..44325
                     /gene="pyrC"
                     /locus_tag="RHV13_000168"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDV1239735.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEHQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            44399..44644
                     /gene="dinI"
                     /locus_tag="RHV13_000169"
     CDS             44399..44644
                     /gene="dinI"
                     /locus_tag="RHV13_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HDV1239736.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            44934..45188
                     /gene="bssS"
                     /locus_tag="RHV13_000170"
     CDS             44934..45188
                     /gene="bssS"
                     /locus_tag="RHV13_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HDV1239737.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            45303..46421
                     /gene="solA"
                     /locus_tag="RHV13_000171"
     CDS             45303..46421
                     /gene="solA"
                     /locus_tag="RHV13_000171"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HDV1239738.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEILVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            46469..46582
                     /locus_tag="RHV13_000172"
     CDS             46469..46582
                     /locus_tag="RHV13_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HDV1239739.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            46843..47409
                     /locus_tag="RHV13_000173"
     CDS             46843..47409
                     /locus_tag="RHV13_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HDV1239740.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            47413..47988
                     /locus_tag="RHV13_000174"
     CDS             47413..47988
                     /locus_tag="RHV13_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HDV1239741.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(48030..49082)
                     /locus_tag="RHV13_000175"
     CDS             complement(48030..49082)
                     /locus_tag="RHV13_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HDV1239742.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDSALDGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARRAREQGLPVRFIGKNFVFDERMGERITDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            49307..50227
                     /gene="lpxL"
                     /locus_tag="RHV13_000176"
     CDS             49307..50227
                     /gene="lpxL"
                     /locus_tag="RHV13_000176"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HDV1239743.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMAVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            50399..51625
                     /gene="mdtG"
                     /locus_tag="RHV13_000177"
     CDS             50399..51625
                     /gene="mdtG"
                     /locus_tag="RHV13_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HDV1239744.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFSVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            51708..52082
                     /locus_tag="RHV13_000178"
     CDS             51708..52082
                     /locus_tag="RHV13_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HDV1239745.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(52083..52310)
                     /locus_tag="RHV13_000179"
     CDS             complement(52083..52310)
                     /locus_tag="RHV13_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HDV1239746.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(52483..55026)
                     /gene="mdoH"
                     /locus_tag="RHV13_000180"
     CDS             complement(52483..55026)
                     /gene="mdoH"
                     /locus_tag="RHV13_000180"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HDV1239747.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWERED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(55019..56554)
                     /gene="mdoG"
                     /locus_tag="RHV13_000181"
     CDS             complement(55019..56554)
                     /gene="mdoG"
                     /locus_tag="RHV13_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HDV1239748.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            56947..58104
                     /gene="mdoC"
                     /locus_tag="RHV13_000182"
     CDS             56947..58104
                     /gene="mdoC"
                     /locus_tag="RHV13_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HDV1239749.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(58112..59593)
                     /gene="clsC"
                     /locus_tag="RHV13_000183"
     CDS             complement(58112..59593)
                     /gene="clsC"
                     /locus_tag="RHV13_000183"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309451.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HDV1239750.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTCEQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIVLKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(59535..60068)
                     /gene="ymdB"
                     /locus_tag="RHV13_000184"
     CDS             complement(59535..60068)
                     /gene="ymdB"
                     /locus_tag="RHV13_000184"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HDV1239751.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(60163..60474)
                     /locus_tag="RHV13_000185"
     CDS             complement(60163..60474)
                     /locus_tag="RHV13_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV1239752.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(60595..60927)
                     /gene="csgC"
                     /locus_tag="RHV13_000186"
     CDS             complement(60595..60927)
                     /gene="csgC"
                     /locus_tag="RHV13_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HDV1239753.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQALHLSQQ
                     WPPSSEKS"
     gene            complement(60986..61441)
                     /gene="csgA"
                     /locus_tag="RHV13_000187"
     CDS             complement(60986..61441)
                     /gene="csgA"
                     /locus_tag="RHV13_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HDV1239754.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(61482..>61934)
                     /gene="csgB"
                     /locus_tag="RHV13_000188"
     CDS             complement(61482..>61934)
                     /gene="csgB"
                     /locus_tag="RHV13_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HDV1239755.1"
                     /translation="KNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFNQ
                     AAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDAS
                     ISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT        15092 a        16508 c        15234 g        15100 t
ORIGIN      
        1 acagagttta ttgtttatta tttaagcgaa tctgtgagag tgatatagag tgaggggtgt
       61 atgcagggcg ttcggtaggc ctgataagag gcgcatcagg cattgatgct cgactgtcgg
      121 atgcggcgta aacgccttat ccgacccaca gaatccggag cgcatcaggt accggcaagg
      181 ttacctgatg cgacgagggc atttacccga gggttttgaa cgctttaatg cgctgtaaat
      241 gcggggagat attcttgaat ttgtgcgtct gctcttcgtc ccagacaatc tcgtaataat
      301 gatgcaactc ttcagatgtc cgctggctgt taagtgcttc atcattacgc gacaaaataa
      361 ccagacaacg atcgcgattc ttctcgcgga agttggtcac acacttagtg gcaatatcgg
      421 catactcttc cgggcgatca atcttgcctt ccatgttctc ataagggaac aaattagggt
      481 tgaagatcac ctggcggatg tcgcaaagaa aaccaatccg ttccgcccag tatccgccca
      541 ggccaacgcc gcaaattagc ggacgctcgt caacgttcag ttgcaacatt ttgtccactt
      601 ctttaagcag atgctgcata tcatgtttcg gatgccgcgt actgtagctt atcaagcgta
      661 catccgggtc aataaactgc aattgtaaga ctttctcgtg gttacccgga ctgttagagt
      721 caaaaccgtg taaatagata atcatcgctt cctcacataa gccagggtta gtgacctgct
      781 ttctcttcct gccagcgttc gtgtaactga ttcagacggg tgctgatcgc tttccagcga
      841 gccgagtcca tcagttcctg tcgcgaaaat gaacctttat gatacaaacg tgtaacacgt
      901 tctgccttga tcggtgacag attatctaac acgctgacgg cccctttacg attattgcag
      961 accaggatca tatcgcaacc cgcatccagt gatgcctgcc cgcgttcggc ataactgccc
     1021 ataatcgcgg caccttccat cgataaatcg tcagagaaaa tcacgccgtc aaaacccagt
     1081 tcctgacgca aaacggtttt cagccagtag gaagaaccgc tcgccggacg cggatcaaca
     1141 tcactgtaga tcacatgcgc aggcataatg gcgtcgagtt tattttcgcg gattaacgtg
     1201 ctgaaaaccg acatatcttt ggcacgaatt tccgcttgcg ggcgtgggtc gcacggcgtc
     1261 tctttgtgtg aatctgcagt tactgcaccg tgtcctggga agtgtttccc ggtcgttttc
     1321 attccggctt catgcatccc atcaataaac cgactggcga ttgccagggc tttttctggg
     1381 tcggcatgat aagaacgctc gccaatcgcc gcgctgatat gcccgacatc cagcactggc
     1441 gcaaagctga tatcaatatc catagcgatc atttcgctgg ccatcagcca acccgcctct
     1501 tgcgccagtt tgccgccctc ttccattccc aacagcgcag caaaggattg tgctgccggt
     1561 aagcgggtaa aaccttcgcg aaaacgctgg acgcgtccac cttcctgatc taccgccacc
     1621 accagatgat tgcgcgatgc tgcgcggatc tggcgcacca gttcacgcaa ctgggcagga
     1681 tcatgatagt ttcgcgtaaa gagaatcagc cctcccacca gcggatgcgc cagtatttca
     1741 cgctcttccg cgtccagttc gtaaccttcg acatccaaca ttactggacc cacactgctc
     1801 tccttattgt tttattaata gctgccgcca ggtgtcatcg gccagcctga taaattgttg
     1861 atcgccggtt tgtcgccagc ggtactcaaa ccaccctgct ttgagcatca gcagccaggg
     1921 aaaccatcgc ctgacctgac gccataattg cgccggataa atcttcgcac gagtggcata
     1981 gtcattgacc aattgccggt gctgttcagt attttccacc cacaccgccg ccagttccag
     2041 cgcgatatca ccatcccctg catactccca gtcgatgagt tttaaccctg acgcgctatg
     2101 cactaaattt ccggcgtgga catccatatg caatggactt aagcgtaaag gccgtggttc
     2161 ccgcgctttg cgcagacgtt ttaacattcg cagccaaccc actgtccgcc gcgccggatc
     2221 gctttgctgc cagtacagtt ccagtaacgg caacagcgtt attcgccagc caaaacgtgg
     2281 ttgttgatgt agataataca gcaagcctgc cagttcgttg gtatccggca aatacgtttt
     2341 taccgcgccg ggcagatagt cgactaccat ccagtcacgg agatataaat gcggcttcgg
     2401 tgcaatgcat gcgggtagtt gtgataaagc ccgatactgg cgcaagaacg cggactgcgg
     2461 cgcatcagga tcgtgcggct gacgcacaac aaaacgctgg ccctgatgtt caatgagaaa
     2521 actcccgcca ctaagcccac tattaaacgt cgtgacggga tgaaactgcg ggaaaaagcg
     2581 cgacagcaat tcgtcgcgcg tgatgggatt attgctgcga aacggcacct ttacctgacc
     2641 agataatttc gcccgtctgc accagcatca gctgcatttg tagggtcgga gcgttaacgt
     2701 tgccagaggc gctggagtac agcacgtaat gagcgccgac attacgggca atgcctatgg
     2761 ctttactacg ggtacctaaa ctgtcctgcg gcgacaaacc taactgttgc ttagccatcg
     2821 acagctgctg ggcggaaacc agggtaaatt taccgttatt agccagcgca tttcgcagcg
     2881 tttcggtcgc ttctgcggca ttcagcgaac cgttagtacg gttgttaacg ctatcaacca
     2941 gaaggacgct acccgcagtc accccgtcag ccccaagcat cttactgacc atcggctgca
     3001 ttgcgccatt ccagtcataa tggcgaatat gcggtgcagg gggtgcagtt tgatcttcgt
     3061 gctcaattgg gcctggctgc tgcgggatcg tcggcaccga gggcactgtg gggacaggct
     3121 gttgtggctc ggctggttgt tccggcgctg gtttcacttc ttctaccggt gcaggttcac
     3181 gttgccccac acacccggcg agaaacatcg ccagcgcggt aatcaaggcg tagcgactca
     3241 tttttgtcat caagattcac cccttacaaa tatagataaa gtctgacttt gtgcgccccc
     3301 agaaaattgg cgctgccgta cagcgttacc gccgaatgtg cgggaatggt aacgctgcgt
     3361 ggcctttcca gaggatgcat ctccagccct ctggcgtcat accagtaaaa acgataatga
     3421 acggtaacgg gttcttgcct ttcgttataa agcgttgagg atgctgaagg ttgaatatca
     3481 gacgtcgaaa ggacgggctt ttctgcactg atgcccgcag ccaataaaga tgactccatc
     3541 accagcgatt gctcatcatt caccggaatt tccggatgtg aacgacagcc caccagcagt
     3601 aacaacacca gagacaccag cccaaagcat ccttttctca tcgttacaga cctttatgcg
     3661 ccagcattgg tcccagcgga cggccaccca acaagtgcat atggatgtgg taaacctctt
     3721 gtccgccatg gcggttggta ttcatgatca gacgatagcc atcttcggca ataccttctt
     3781 gctcagcaat ttttgccgct acggtgatca tgcgtcccag cgcctgctca tgctcagctg
     3841 acacgtcgtt cacagtcgga atgaggatat tcggaatgat cagaatatgc gttggcgctt
     3901 gcggcgaaat atcgcgaaac gccgttacca gatcatcctg gtagacgata tcggagggga
     3961 tctcacgacg aataattttg ctgaatatag tttcttctgc cacgacattt tcctttttca
     4021 aatagccatg cgttgcgcca tgctacgccg cgccacactg cgagtataga gtatggtcga
     4081 gttaaacctg ctctttcaac cttaactcac gatttcttaa gcaaaaaatc gttttgctgc
     4141 cgtatatatc gccattattc ccatttctgc ctgcgaatgc gctgaaaaga cattctctgt
     4201 ttggtttaca cgacaaatat gaatgcgtat atttctcatt tgcatttaca aacaaaatta
     4261 ttcgcacact aaatataacc gctcatcacc ctcctccggc atgagcctga cgacaacata
     4321 aaccaagaga tttcaaatgc tttcaacaca atttaacagg gataatcaat atcaagccat
     4381 caccaaaccc tcactacttg ccggttgcat agcattggca ctattacctt ctgccgcttt
     4441 tgctgcacca gccactgaag aaacggtgat tgttgagggt tcagccacag ctccagatga
     4501 tggcgaaaat gattacagcg taacgtctac ctctgcgggt accaaaatgc agatgactca
     4561 acgtgatatt cctcagtcgg tcactattgt tagccagcag cggatggaag atcagcagtt
     4621 acaaacgctg ggcgaagtga tggaaaacac gctggggatc agcaaaagtc aggcggattc
     4681 cgatcgtgct ctttattatt cccgcggatt ccagatcgat aactatatgg ttgatggtat
     4741 ccccacctat tttgtatcgc gctggaatct gggcgacgca ctttctgata tggcactgtt
     4801 tgaacgcgta gaagtagtgc gtggcgcgac aggactcatg accgggacgg gtaatccatc
     4861 tgcggcaatt aatatggttc gaaaacacgc gaccagtcgt gaatttaaag gcgatgtctc
     4921 ggcggaatac ggtagctgga acaaagaacg gtatgtggcg gatttacaaa gcccactcac
     4981 cgaagacggt aaaatccgcg cgcgaattgt cggcggctac cagaataacg actcatggct
     5041 ggaccgctac aacagtgaaa agaccttctt ctcgggcatt gtcgatgctg atttaggcga
     5101 tcttacgacg ctttcagccg gttacgaata tcagcgcatt gatgttaata gcccaacctg
     5161 gggcggttta ccgcgctgga atactgatgg cagcagcaac agttacgatc gcgcacgcag
     5221 taccgcacct gactgggcgt acaacgataa agagatcaac aaggtcttta tgaccctgaa
     5281 gcagcggttt gctgatacct ggcaagcgac gctgaatgcc acccactctg aagtcgaatt
     5341 tgacagcaaa atgatgtatg tcgatgccta tgtaaacaaa gcggatggta tgctggttgg
     5401 gccatacagt aattatggac ctggctttga ttatgtcggc ggcaccggtt ggaacagtgg
     5461 caaacgtaaa gttgatgcgc tggatttgtt cgctgacggt agttatgaat tgtttggtcg
     5521 tcagcacaat ctaatgtttg gtggcagtta cagcaaacaa aacaatcgtt acttcagttc
     5581 atgggccaac atcttcccgg atgaaattgg cagtttctac aactttaatg gcaatttccc
     5641 acaaaccgac tggtcaccac agagcctggc gcaggacgat accacacata tgaaatcgtt
     5701 atatgctgcc actcgtgtca cccttgccga tccgctgcat ctgatcctcg gcgcacgtta
     5761 taccaactgg cgggttgata cgctgactta cagcatggag aaaaaccaca ccacgcctta
     5821 cgctggtctg gtgtttgaca tcaatgacaa ctggtcgacc tacgccagct atacctctat
     5881 tttccagccg caaaatgatc gtgacagttc aggcaaatat ctggctccaa tcaccggtaa
     5941 caactacgag ctgggtctga aatcggactg gatgaatagc cgtctgacca ccacattagc
     6001 catcttccgt attgagcagg ataatgtcgc tcagtccacc ggtacaccta tccccggcag
     6061 caacggcgaa accgcctata aagcggtgga tgggacagtc agtaaagggg tggaatttga
     6121 actcaacggc gcaattaccg acaactggca gctgacattt ggcgcaacgc gctatattgc
     6181 agaggataac gaaggaaacg ccgttaatcc taatctgcca cgcaccacgg ttaaaatgtt
     6241 caccagctat cggttgcctg tcatgccaga gttgacagtc ggcggtggtg ttaactggca
     6301 aaatcgcgtg tataccgaca ccgtgacacc gtatggcacc ttccgcgccg agcaaggtag
     6361 ctacgcgctg gtggatctct tcacccgcta ccaggtgacg aaaaacttct cgttacaggg
     6421 gaacgtcaat aacctgttcg acaaaaccta cgataccaac gtggaaggtt ctatcgtcta
     6481 cggcgcaccg cgtaatttca gcattaccgg cacgtatcaa ttctgaataa cacctgtaaa
     6541 aaaggcagcc atctggctgc cttagtctcc ccaacgtctt acggattagt ggttacggat
     6601 gtactcatcc atctcggttt tcaggttatc ggatttagta ccgaaaatcg cctgaacacc
     6661 agaaccagca accactacgc ccgctgcgcc cagtttcttc aggccggcct gatccacttt
     6721 agacacatca gcaacgctga cgcgcagacg ggtaatacat gcgtcgaggt tagtaatgtt
     6781 ttctttacca ccaaatgcag caaccagagc cggtgccatt tcgctggtac ctgtcgcttt
     6841 tgcatcttca gtcgcgtctt cacgacccgg cgttttcaga tccagtgctt taatcagcac
     6901 gcggaagatg gtgtagtaaa caatcgcata accgataccg acgatcggga acagccacag
     6961 tttgctgctg ttaccagaca gaacgatgaa gtcgatcaga ccgtgcgaga acgacgtacc
     7021 gtcacgcatc cccagaagaa tacagattgg gaatgccagg cctgccagaa tcgcgtggat
     7081 gatgtacagg atcggcgcaa cgaacatgaa ggagaactcg atcggctcgg tgataccggt
     7141 caggaacgag gtcagcgccg cggagatcat aataccgccc actttcgcgc ggttttctgg
     7201 tttagcagag tgccagatag caattgcggc agctggcaga ccgtacattt tgaacaggaa
     7261 gccaccagac agtttacccg cagtcgggtc acccgccata taacgcggaa tgtcgccgtg
     7321 gaaaacctga cctgctgcgt tggtgtattc accaatctgc atctggaaag gtacgttcca
     7381 gatgtggtgc agaccaaacg gtaccaggca acgttcgatg aaaccgtaaa tgccaaacgc
     7441 aactaccggg ttctggtaag cagcccactg agagaaggtc tggattgcag aaccaatcgg
     7501 cggccaaatg aaggacagca caacgccagt aaagatggca gccaggccag aaatgatcgg
     7561 cacaaagcgt ttaccggcaa agaagccaag atactcaggc agcttaatac ggtagaaacg
     7621 gttaaacatg tacgctgcga tcgcaccgga gataatccct ccgagtacgc cagtatccgc
     7681 caggtgttta gaggcgattt cttcagcagg taaatgcagt accagtggcg caaccacggc
     7741 catggtttta accatgatgc cataggcaac aactgcggcc agcgcggata cgccatcgtt
     7801 attggtaaag ccgagggcga caccgatcgc aaaaatcagt ggcatgtttg caaagacgga
     7861 accgcctgct tctgccataa catgcgatac aacggcgggc agccagctga aattcgcgga
     7921 accgacgccc agcagaatac ctgcgatagg cagtacggat accggcagca tcagcgattt
     7981 accgaccttt tgcaggttag caaatgcatt cttaaacata attgagagtg ctcctgagta
     8041 tgggtgcttt ttttacgttc tcacgcgtgg caagggggga gagcctcgcc gtgtacaggg
     8101 catctaagcg ccctttattt attacacaga gtaaaataat tcagtgccaa tttgtttgac
     8161 ggctatcacg tttcaacttc agatcattgg agaagttgca cattttgaca aaacatatgt
     8221 cacaatattt caaacgcggt tattcaccgc ccaattttcg gcggtgaact ttactcgttt
     8281 tagccattaa ttacgagctt taaaaaaact cattcaacgg atggattgaa ggcgggaagc
     8341 gtcgatgtga aacagacggg cgaagttatc ggtggttacc tgcgccagtt cttcaacggc
     8401 aacacctttc aacacagcca tgtattctgc aacgtcacga accatcgcag gttgattctc
     8461 ttttcctcga tgcggcaccg gcgcaaggta aggtgagtca gtttccacca gtaaccgatc
     8521 cagggggaca taacgcgcag catcgcgcag ttgctccgca ttacggaagg tcacaatgcc
     8581 ggaaaaggag atgtaaaatc cgagatccag taatttgccc gccgtttctc tgtcctctgt
     8641 aaaacagtgt agtacgccac cgcaatccgt cactttttct tcgcgcagaa ttgccagcgt
     8701 atcggcgcgg gcgtcacggg tatggacgat aaccggcttg ttcagttcac gaccaatctg
     8761 gatatgatgg atgaaggact cttgctgacg tactttagtt tccggtgtgt aataataatc
     8821 cagcccggtt tcacccagcg ctacaacacc ctcttctgcc gccagacggc gtaaatcttc
     8881 tacatcgtag ggatcattct ggttgagtgg atgcacgcca caagaaaata cgacgttgtc
     8941 acgttcgcct accagatccc gcatatgtaa ataacccggt aatgttgtgg cgactgccag
     9001 acaaaatttc acatcgcgtg cggcggcttt cgccagaacg tcatccacgt ccttatgcaa
     9061 agattcataa tccagaccat cgagatggca gtgtgagtcg actaaaaaca tgatgtctct
     9121 cttaaagatg aggaaccggt agcacaacgc ccggttgcag gtaatgctca atacgcagta
     9181 aaagatcggt gatgagaagc tcgcggttga tgcctgtaac agacattaac tgttcacgaa
     9241 tgtggcaaac atcccccagt atagcctgca ggcgcgaggg agaaagatgg tttgccagtt
     9301 cgacgaccag gcccggcaca tcaacattgg tcacctgcgc agcgccatga tggcgtttta
     9361 gcgcatccat cagcaacgtt gccagccagt gcaaacgcgc cggagcttgt tcatgattaa
     9421 gggccgccag cagcgaatac cagtcgcccg atggcacgct atatgccaac gcctgacaca
     9481 atgtttcacg agcctgccag ttatctccct gaaacaacgc cagtgccgcg ccaggcgaac
     9541 cggcgcttaa gcgcaatgcg gcaagtaatg caacctgtga cattgtcact tcgcgtgaaa
     9601 gccaggtcac ggcgtactgt tccggcggcg gcgcaaggta atgtaaccga caacgactac
     9661 gtaatgttgc cagtaaacgt tcaggctcgc gggtagccag gaaaaaccag gtttctgctg
     9721 gtggctcttc aagcgttttc agcaatgcgt tagccgcggc gtcggtcagt aaggcagcat
     9781 cggttaccca gacaactttc gcaccaccta ggcgtgcgtg ctcattcagc ttttcggtga
     9841 cctcacgtac cgcatcaatg cccagcgtat ttttcccttt ttcgggagcc agggtgtagt
     9901 aatcgggatg cgtgccagcc tgcatcaact gacatccacg acagtgaccg caacttttgt
     9961 ggccctgcgg ttgttggcag agtaaataac ggctcagggc gtaaattaaa gcatcatcgc
    10021 ccatgcccgg taacgcctga atgagtagcg catggtgacc tcttccggcc tgatagctgg
    10081 ctaccagttt ttcgaaatca ggtcgtaacc atggatacca tctcatgcgt ccaactcctt
    10141 cacccagtgg gtcacggtag tgcggattgc atccatcacg gcctccagcg gctgggtggc
    10201 atcaatggta tgaatacttt tatcttgcgc agccagttcc agatagcggg cgcgggtgcg
    10261 attaaagaaa tcgaaagact cttgctcaat acgatccagc tcgccgcgcg cacgcgcgcg
    10321 ttttaagcca acttccgggg taacatcgag atagagcgtt aaatcagggc gaaaatcccc
    10381 gagaacagca tcacgcagtg ttgccagcat atgttggtca ataccacgtc ctccgccctg
    10441 atacgcctga gtggagagat cgtggcgatc gccaatcacc caggtgccgt tagccagcgc
    10501 aggtttgatg accgtttcta ccagttgaac gcgcgcggca taaaacatca gaacttcggc
    10561 tttatcggta atgacttcat cgcctaccga tttgatatcc agcaccaggc ttcttaactt
    10621 ttcggcaagt tgcgtaccgc caggttcccg agtgaaaacc atgtcgcgga tacccagttg
    10681 ctcaagcgtc tcaaccacca cattacgcgc ggtagttttg cctgcgcctt ccagcccctc
    10741 aatgacgata tacttactgc gcattttttt ccttaagcac tttcagataa tcctgcacag
    10801 acttgttatg actggcaaga ttggtattaa acgtgtgacc acctttacca tcggccacaa
    10861 aatagagata cggcgttttt gccggatgcg cagcagcctt cagcgaatcc gcccccggcg
    10921 tagctatcgc acccggcggc aaaccggtaa tggtataggt gttatacgct gtcggcgttt
    10981 ccaggtctgc acgagaaagt ttgccattat aacgctctcc catcccgtaa atcacggttg
    11041 ggtcggtctg caggcgcata ccaatgcgta aacggttgat aaataccgag gcaacctgat
    11101 cgcgttcact ggcaacggcg gtttcttttt cgataattga tgccatcgtc accagctgat
    11161 ttttatcttt ataaggcaga ccgtccgcac gcccttccca ggcgctatcg accgctttca
    11221 ccattttctt gtgcgctcgc ttgagtaacg cgacatcggt ggtattggcg gtatacatcc
    11281 aggtgtctgg ccagaaccaa ccttcaatcc actccgggtt ttccagttca agtgcctgcg
    11341 ctacggtgat gtacttatca tcgctcagcg tgtgcttgat atacggggcc tcacgcaatt
    11401 gcttgaggta gtcgctcaga cgcatccctt ctaccagtcg cagcgggaac tgtgcttctt
    11461 taccgctttc caacaatttc agcatctcgc gcacggtcat ctgcggtgta aagcggtaag
    11521 tcccggcttt aaagtgagaa agatccggtt cgatacgcag caaccattga aaaacccgtg
    11581 ggcgattgat gatcttatcg gcataaagct gttcacctag cgccagacgt ccggtccctg
    11641 gcttcagggt aaatatcgtc tcttctttga taagcaattt gctgtcggca agatggcgaa
    11701 ccttccagat gcccacacca gcggcgatac ccagtaccac cagcaataac aagattatca
    11761 ataacacttt tttcatgact aattcgggcg ctcacaaagt ggggctaaat attcatataa
    11821 cgttgctgac gaaaaggaga catcgccaca ggcacgtacg gacatcactg gcattaacgc
    11881 attacaaata accatctcat ctgcctgcaa cgcctcttcc agagaggctt gcacttcgac
    11941 aagctgataa gaggattgtg ccagcaaacg gatacagaat tgtcgcataa tgccgttaac
    12001 acctgcctga tccagtcgcg gcgtataaac tacgttgccc ttccgccaga acaaattagc
    12061 cgcacagcat tccgtaaccc acccttcgct gtcaaggacc agcgcctcat cagcgtttgt
    12121 ctgctcaaga tgagagcgga tcaatacttg ctcaagccga ttaagatgtt taatacctgc
    12181 aagatgagga ttgcgcccca gccgcaccgg gcttagcgcc aacgtcatcc cttcgttacg
    12241 caaacggtcg taatgtgcag gataagccgt aacggagaga atccgcgttg ccggtccact
    12301 gttcaatgtg ctgtaccctc gcccgccact accgcgactg atcacgactt tcagtacacc
    12361 attttgctgt tctgctgcca gcgttttcat ctcttgttca agctgaggcc agaagtcaca
    12421 ggaaatcatc aaccgctgac aagcatcctg tagtcgctgg atatgcgccg ataacaaact
    12481 gactttaccg tcgataactc tggcggtggt aaaacaacca tcaccaaact gcgttgcccg
    12541 atcgcttact gccagcgatt cctgcttata accgttaatt aagaacatac cggctcctta
    12601 tcagagaatt gttagtgtgg cagcatgttc actacggaac aagtcggaat aaaagctaag
    12661 aaaaaaggcc cgcaagcgga ccttttataa gggtggaaaa tgacaattta gatcttttta
    12721 aagatcaaag aaccattagt gccaccgaag ccgaaggagt tacacagcgt gtattccatt
    12781 ccgctaacct gacgcgcttc gtgcggtacg aaatccagat cgcaaccttc atccgggtta
    12841 tccaggttga tggttggcgg aacagcctga tcgcgcagcg ccaggatgga gtagatagat
    12901 tctactgcac ccgccgcacc taacaggtga ccggtcatag atttcgtgga gcttaccaac
    12961 acacggcttg cagcttcacc gaagatggtt ttcaccgcct gcgcttcagc tttatcgcca
    13021 gccggcgtag aagtaccgtg cgcgttaacg tagccaatct gactcgcttc aatgcctgca
    13081 tcacgcagag catttgccat cgccagagct gcgcctgcgc cattttctgg cggtgacgtc
    13141 atatgataag catcgctgct cataccaaag ccgacgagtt cagcgtaaat tttcgcaccg
    13201 cgttttttcg cgtgttcgta ctcttcaagt accagcatac cggcaccatc gcccagtacg
    13261 aagccatcac gctctttatc ccacgggcgg ctcgccgctt gcgggttatc attgcgggta
    13321 gataatgcac gtgccgcgcc aaaaccacca acacccagcg gcgtactggc tttctctgcg
    13381 ccacctgcaa ccatcacgtc agcatcgcca tacgcgataa tacgcgcagc atggccaatg
    13441 ttgtgcacgc cggaagtaca ggcggtcgcg atagagatgc ttgggccacg caggccatac
    13501 atgatagtca gatgacctgc caccatgttc acaatcgttg acggaacgaa gaatgggctg
    13561 atcttacgtg gaccaccgtt catcagagat gtgtggtttt cttcgatcag tccgaggccg
    13621 ccaatcccgg agccaattgc ggcaccaatg cgggttgcgt tctcttccgt tatttcaagg
    13681 ccagaatcct gcatggcctg aacgccagcg acaattccat attgaatgaa ggcatccatc
    13741 ttgcgctgtt ctttgcgcga gataatgtcc tcacagttaa aatcctttac taagccagca
    13801 aatttcgttg cataggcgct agtatcgaaa tggtcgatta ggctgatgcc actctgaccg
    13861 gcaagcagag ctttccaggt agactctacg gtattgccga caggagacaa catgcccagt
    13921 ccggtcacaa ctacacgacg cttagacacg tttgtcctcc agggagggaa aaaatgattc
    13981 tagtgggaca aaaagataaa actcaggcgg tcgaacgacc gcctggagat gttcacttac
    14041 gcctggtggc cgttgatgta atcaatggca gcctgaacgg tggtgatttt ctcagcttct
    14101 tcgtccggaa tctcagtatc aaactcttct tccagagcca ttaccagctc aacggtgtca
    14161 agagaatccg cgcccaggtc ttcaacgaaa gaagcattgt tggtaacttc ttcctgctta
    14221 acgcccagct gttcgccgat aattttctta acgcgttctt cgatagtgct catactctta
    14281 aatttcctat caaaactcgc tttcgcgatg gttttcgtag tgtataaaat gttgaaaaat
    14341 ttgcaactaa atcccggcag gtcttaccac gattttacgt tattttgagg ccttaccccc
    14401 caataacgca aatcattttg cactaattat tactcataac cacgcagact gcgcgcaaca
    14461 tgagcatttt gtgcaaatcg cggtcagacc atgtacatcc cgccgttcac atgcaaagtt
    14521 tcacccgtga tgtaagctgc ttcgtcggat gccaggaatg caaccgcgtt ggcgatttcc
    14581 tgtgcgccgc cgaggcgacc cgcaggaacc tgcgccagga tacccgcacg ctggtcatcg
    14641 ctcagcgcac gtgtcatgtc cgtttcaata aagcccggag caacaacgtt tacagtaata
    14701 ccgcgtgacg caacttcgcg cgccagtgat ttactgaagc cgatcaagcc cgctttcgcc
    14761 gcagcgtagt tggcctgacc gccatttccc atggtaccaa ccacagaacc gatagtgata
    14821 atacgaccat gacgcttttt catcatagcg cgcattaccg cttttgacag acggaaaaca
    14881 gatgaaaggt tggtttcgat aatatcgttc cactcttcat ctttcattcg cattaacagg
    14941 ttatcacgag tgataccggc attattgacc aggatatcca cttcaccaaa ttctgcgcga
    15001 attttttcca gaacagattc gatagatgcc gggtcggtca cattcaacat cagacctttg
    15061 ccgttagcac ctaaataatc actgatcgcc tgagcgccat tttcactggt cgcagtgcca
    15121 ataactttcg cgccacgggc tgcgagcgtt tcagcaattg cgcggccaat tccgcggctt
    15181 gcaccggtta ccagtgcgat ttttccttca aaattcatga ttttcctctt ttaaagctcg
    15241 agcgccgctg ccatcgctga aggttcgttc agcgccgagg cggtcagggt gtcgacaatg
    15301 cgtttcgtca ggccagtaag cactttgccc gggccgactt catagagatg ttctacgcct
    15361 tgcgctgcca tgtactcaac agacttcgtc cactgaaccg ggttatacaa ctgacgtacc
    15421 agtgcgtcac gaatggcatc accattggtt tcgcatttca catcaacgtt attcacaaca
    15481 ggaactgttg gtgcgttaaa ggtgattttc gctaattcta ctgccagttt gtcggctgct
    15541 ggtttcatca gcgcacagtg agacggtacg ctcactggta acggcagagc gcgttttgcg
    15601 cccgcagctt tacaggcagc gccagcacgc tcaaccgctt ctttatgacc ggcaataacc
    15661 acctgtcccg gagagttaaa gtttaccgga gaaacgacct gaccttctgc agcttcttca
    15721 cacgctttcg caatagacgc atcatccaga ccgatgattg ccgccatagc gcccgtacct
    15781 tccggtacgg cttcttgcat gaacttgccg cgcatctcaa ccagacgcac cgcatcagcg
    15841 aaatcaatca caccagcgca aaccagcgcg gagtattccc ccaggctgtg accggccatc
    15901 aatgccggtg ctttaccgcc ctgctgctgc catacgcggt acagcgcaac agatgcagtc
    15961 aacagcgctg gctgggtttg ccaggtttta ttcagttctt cagctggccc ctgctgggtc
    16021 agcgcccaca ggtcgtagcc cagcgccgca gaagcttcag caaacgtttc ttcgacaatt
    16081 ggatagctcg ccgccatatc agccagcatt ccaacggttt gagaaccctg tccagggaac
    16141 acaaatgcaa attgcgtcat gttttaatcc ttatcctaga aacgaaccag cgcggagccc
    16201 caagtgaacc caccgccaaa ggcttcaagc agaaccaact gccccggctt aatgcgcccg
    16261 tcgcgtacag cttcatccag cgcgcacggg acagaggccg cagaggtatt accgtggcga
    16321 tccagcgtca ccacgacatt gtccatagac ataccgagtt ttttcgccgt tgcactgata
    16381 atacgcaggt tagcctgatg cggaaccagc cagtccagtt gagaacggtc aagattattc
    16441 gccgccagcg tctcatcaac gatgtgcgcc agttccgtta ccgcaacctt gaagacttcg
    16501 ttgcccgcca tcgtcagatg aattgaattc tctggattca cgcggtcggc gtttggcagc
    16561 gtcagcaatt caccataact accgtcggca tgcagatggg tggagatgat tcccggctct
    16621 tcagaggcag ccagcaccgc agcgcccgca ccatcgccaa aaataataat agtcccacga
    16681 tctgttggat cgcaggtgcg cgccagtaca tcggaaccga cgaccagagc atacttcacc
    16741 gccccagatt tcacgtattg atcggctacg cttaatgcat aggtgaaacc tgcgcaggct
    16801 gctgcaacgt caaatgccgg gcaaccttta atgcccaaca tgctttgaat ctgacaagct
    16861 gcgctcggga aagcgtgcgt agcagaagtc gttgccacaa cgatcaggcc aatctggtct
    16921 ttctcaatgc ccgccatctc aattgcgcgt gtcgccgctt caaagcccat ggttgaaacg
    16981 gtttcgtttg gcgcggcaat gtggcgttcg cggataccgg tacgagtgac aatccactcg
    17041 tcagaggtgt ccaccatttt ttccaaatcg gcgtttgtcc gcacttgttc gggcagatag
    17101 ctgccagtac caataatctt cgtatacatg tacgctcagt cacttttcgg ttatataccg
    17161 tcacttgcaa actgcgagtt cgctggcagc gtcctgctac cgcagagttc cgcttttgcc
    17221 accgtccagc agctcaaaac cagctgggta tacagattcc aggcgagcgg caattcgctg
    17281 aggaacttgt cgctgcaccg cctgcactgc ctgttcaatc gcgaccgcaa aagctcgctg
    17341 attggctgca ccatgacttt ttatcaccgt gccgcgcaat cctaacagac aggcgccgtt
    17401 atactggtcg gggttgaggt gactgaatcg cctcgtcagg ctcttttgta gccaacgctt
    17461 taataacagt agccaccacg accgtttttt cccttcaccc tgagatttca gcagagaaag
    17521 gaacatcctg acaacacctt ccatcgtctt taatgtgaca tttcctgtaa agccgtcaca
    17581 aaccagcaca tctgtcttgc cagttaacaa ctcattggct tcaagatagc cgatataatt
    17641 gatagaaggg attgttttaa gcaccgctga ggcatcccga atactatcga gaccctttac
    17701 ttcttcttca ccaatattga gcaacgccac gcgaggattg ggaatttcca ccacctcttc
    17761 agccaggact gagcccataa tggcaaattg caccaacatt gtgctgtcac aatcgacgtt
    17821 ggcccctaag tcaaggacca ccgttttgcc cttttgctga tgtggtaata ccgtcaccag
    17881 cgccggacgc tcaatcccct ccaggggctt gagtaataat tttgccagcc ccatcagtgc
    17941 cccggtatta ccggcactga cacaggcttg cgctcgacct tctttcacca gctccagggc
    18001 cacgcgcatt gaactcccac gactggcgcg gatagcttgc gaaggccggg catcactggc
    18061 gataactgac tgcgcaggaa ttatctgcag acgcgaacgt tgttcaaagt cagctttagc
    18121 aagtaatggc gtgatggcgt cgggattgcc gactaaaaga agagtgagtt gcgaattaga
    18181 attcagtgcc tgcaatgctg caggcactgt cacggaaggg ccaaaatccc ctcccatgac
    18241 atctaacgcc agggttagac gtgtcaaggt atcgtcgccg cccggtttgg tctttcccca
    18301 gttgcctggg gaaaatcctc actaagcttc atcacgcaga cgcgtgatta cttagcgatg
    18361 accttgcggc cgcggtagta accgtcggca gtgatgtggt gacgcaggtg tttttcacca
    18421 gaagttttgt ctacagacag gctggtgact gcggtcagcg cgtcatggga acgacgcatg
    18481 ccacgtttgg aacgggttgg tttattctgt tgtacggcca tggaccttac tcctcaatta
    18541 cggtttatcc ccgatccaac ggggagcacc aattacttac gctttaagct ggctaatacg
    18601 gcaaatgggt ttggcttttg cgcttcttca ggcagttcac caaagaccat gtccgcttcg
    18661 gacacttcac agtgttcaga atcatgcacc ggaactaccg gcaaggcgag gatgatttca
    18721 tcttcaacca ttgcaagcag atcgatttca ccgaattcgt taacctcaat cggttcatac
    18781 gcttccggca gtgcttcagc ctgttcgtct gaacgcacag gactaaaaca atacgttgtg
    18841 tagacctgat gagtaaacgg cttcccgcaa cgctgacact cgagcgttac cgtcaccttc
    18901 gcatcgccgt ttaacactgc gagacgttgg ttatcgatag cgaacgacat ggagcattcc
    18961 acatcactgt ccacactgac tacggattcg gcgacgcgct caacctgatc aggggtatag
    19021 ataccctggt aatcaaggcg tttttgagcc gtacgaaccg gatcgagagt caggggtaat
    19081 tttacctttt gcatagggcg cgcatattaa ctttgtaacg tcatagagtc aaagaaaaag
    19141 gcagcctgtg gttgcctttt gccaataatt cgcacacatt gcgggttact gctttatttt
    19201 cgttcagtgc tgataacgaa catcaacctg caaaacgaag cgcatagttt aaaatggcta
    19261 tcatcaatac gctatatctg atggaaaaaa atatgcctaa acttatttta gcctccacct
    19321 cgccctggcg tcgcgctctt ctggaaaaac tgcaaatctc ttttgaatgt gcagcacccg
    19381 aggtcgacga aactccacac agcgacgaat caccgcgaca gttggtgctt cgactggcac
    19441 aagaaaaagc gcaatctctg gcgtcacgtt atccggatca tttaattatt ggttcagacc
    19501 aggtatgtgt tcttgatggc gaaatcaccg gtaaaccgtt aacggaagaa aatgcccgtc
    19561 tgcaattacg caaagccagc ggcaatatcg tcaccttcta tactggactg gcgttgttta
    19621 attcggcgaa tgggcatcta caaacagaag tggagccttt tgacgtccat ttccgtcacc
    19681 tgagcgaggc ggagattgat aattacgtgc gtaaagagca tcccctgcac tgcgcgggta
    19741 gctttaagag tgaaggattt ggcattacgc tgtttgagcg tttagagggg cgtgatccta
    19801 acacgctggt tggtttaccg ctaatcgcgc tgtgtcagat gttacgtcgg gaagggaaaa
    19861 acccgctgat gggatagttt tttgcgccgg atgtggttac ttcgcatccg gcaatcggtg
    19921 ctggcggcct gatgcggcgt aaacgcctta tcaggccaac cgcccattat caggtttata
    19981 tgcttgtatt agcgcgcgtt acgcagcttt tgcaaacaac gcttcaaacc atcatccatc
    20041 ggcgcttcga tacgcatcac ctcaccggtc cccggatggg taaacttcaa cgctgcggcg
    20101 tgcaggaaca gacgatttaa tcccgtgcct gcttcagtga gctgtctgtc aaattcacgg
    20161 tcaccgtagc gatcgtcaaa ggcaatcgga tgacctgcat actgtgtatg cacacggatc
    20221 tgatgagtac gccctgttac cggactacaa cgcaccaggg tggcaaatgc atagcgttct
    20281 tccactttaa agcgtgtttc cgacggtttg ccttcctgac tcacacgcac gatacgttcg
    20341 ccgctttgca gaatattttt cagtaacggt gcttgaacgc tcttcacatg cgactgccac
    20401 tgaccgcgca ccagcgccag gtaatctttt tgcatccctt tttcacgtaa ttgctcatgc
    20461 agagaacgca acgccgagcg ttttttcgct accagcaaca cacctgaggt gtcccggtca
    20521 agacgatgaa ccagttcaag gaaccgcgct tccgggcgca acgcccgcaa accttcaata
    20581 acgccgaagc ttaaaccact gccgccatgt accgccgtac cggaaggttt attcagcacc
    20641 aggatgtgat catcttcata taagatgacg tccgccagcg ccgccacctt ttgcagatgt
    20701 ggtgaaaccg cctcttcttc ccgctcagca acgcgaaccg gtgggatacg cacctcatca
    20761 cccgcttcga gtttatattc aggcttaata cgttttttgt tcacccgcac ttcgccttta
    20821 cgcaaaatac ggtaaatcat actttttggt acgcctttca attgggtacg caaaaagtta
    20881 tcgatacgtt gccccgcttc gtcagcggtg atagcaacaa tttttacgga tggagtctct
    20941 gttttcatgg ttggcgattc taattagcca acaggattcg cgccactcat ttttctatgc
    21001 ttatatttac ttttgcacct tattacttca ctgcgtgatc actttattga tggttattaa
    21061 accaatcacc agcaagaagt gaaaaaactg tgagtaagcg ggtgataaat ggtaaaagtc
    21121 atcttgctat aacaaggctt gcagtggaat aatgaggccg tttccgtgtc catccttgtt
    21181 aaaacaagaa attttacgga ataacccatt ttgcccgacc gatcatccac gcagcaatgg
    21241 cgtaagacgt attgatcttt caggcagtta gcgggctgcg ggttgcagtc cttaccggta
    21301 gatggaaata tttctggaga gtaataccca gtctgtttct ttgataattg cgctgttttt
    21361 ccgcatgaaa aacgggcaac cgacactctg cgcctctttg agctgacgat aaccgtgagg
    21421 ttggcgacgc gactagacac gaggccatcg gttcacaccc ggaaaggcgt tactttgccc
    21481 gcagcttagt cgtcaatgta agaataatga gtaagttacg atgaaaagaa tgttaatcaa
    21541 cgcaactcag caggaagagt tgcgcgttgc ccttgtagat gggcagcgtc tgtatgacct
    21601 ggatatcgaa agtccagggc acgagcagaa aaaggcaaac atctacaaag gtaaaatcac
    21661 ccgcattgaa ccgagtctgg aagctgcttt tgttgattac ggcgctgaac gtcacggttt
    21721 cctcccacta aaagaaattg cccgcgaata tttccctgct aactacagtg ctcatggtcg
    21781 tcccaacatt aaagatgtgt tgcgtgaagg tcaggaggtc attgttcaga tcgataaaga
    21841 agagcgcggc aacaaaggcg cggcattaac cacctttatc agtctggcgg gtagctatct
    21901 ggttctgatg ccgaacaacc cgcgcgcggg tggcatttct cgccgtatcg aaggcgacga
    21961 ccgtaccgaa ttaaaagaag cactggcgag ccttgaactg ccggaaggca tggggcttat
    22021 cgtgcgcacc gctggcgtcg gcaaatctgc tgaggcgctg caatgggatt taagcttccg
    22081 tctgaaacac tgggaagcca tcaaaaaagc cgctgaaagc cgcccggccc cgttcctgat
    22141 tcatcaggag agcaacgtaa tcgttcgcgc attccgcgat tacttacgtc aggacatcgg
    22201 cgaaatcctt atcgataacc cgaaagtgct cgaactggca cgtcagcata tcgctgcatt
    22261 aggtcgcccg gatttcagca gcaaaatcaa actgtacacc ggcgagatcc cgctgttcag
    22321 ccactaccag atcgagtcgc agattgagtc cgccttccag cgtgaagtcc gtctgccatc
    22381 cggtggttcg attgttatcg acagcaccga agcgttaacg gccatcgaca tcaactccgc
    22441 acgcgcgacc cgcggcggcg atatagaaga aaccgcgttt aacaccaacc tcgaagctgc
    22501 cgatgagatt gctcgtcagc tgcgcctgcg tgacctcggc ggcctgattg ttatcgactt
    22561 catcgacatg acgccagtac gccaccagcg tgcggtagaa aaccgtctgc gtgaagcggt
    22621 gcgtcaggac cgtgcgcgta ttcagatcag tcatatttct cgcttcggcc tgctggaaat
    22681 gtcccgtcag cgcctgagcc catcactggg tgaatccagc catcacgtct gcccgcgctg
    22741 ctccggtacc ggtaccgtgc gtgacaacga atcgctgtcg ctctctattc tgcgtctgat
    22801 cgaagaagaa gcgctgaaag agaacaccca ggaagttcac gccattgttc ctgtgccaat
    22861 cgcttcttat ctgctgaatg aaaaacgttc tgcggtgaat gccattgaaa cgcgtcagga
    22921 cggtgttcgc tgcgtgattg tgccaaacga tcagatggaa accccgcact accacgtgct
    22981 gcgcgtgcgt aaaggggaag aaacgccgac cttaagctac atgctgccga agctgcatga
    23041 agaagcgatg gcgctgccgt ctgaagaaga gttcgctgaa cgtaagcgtc cggaacaacc
    23101 tgcgctggca acctttgcca tgccggatgt gccgccagcg ccaaccccag ctgaacctgc
    23161 cgcgcctgtc gtagccccag cacctaaatc tgcaccggca acaccagcag ctcctgcaca
    23221 accagggctg ttgagccgct tcttcggcgc actgaaagcg ctgttcagcg gtggtgaaga
    23281 aaccaaaccg tccgagcaag cagcaccgaa agtagaagcg aaaccggaac gtcaacagga
    23341 tcgtcgcaag cctcgtcaga acaaccgccg tgaccgtaat gagcgccgcg acacccgtag
    23401 tgaacgtact gaaggcagcg ataatcgcga agaaaaccgt cgtaatcgtc gccaggcaca
    23461 gcagcagact gccgagacgc gtgagagccg tcagcaggct gaggtaacgg aaaaagcgcg
    23521 taccgccgac gagcagcaag cgccgcgtcg tgaacgtagc cgccgccgta acgatgataa
    23581 acgtcaggcg caacaagaag cgaaggcgct gaatgttgaa gagcaatctg ttcaggaaac
    23641 cgaacaggaa gaacgtgtac gtccggttca gccgcgtcgt aaacagcgtc agctcaatca
    23701 gaaagtgcgt tacgagcaaa gcgtagccga agaagcggta gtcgcaccgg tggttgaaga
    23761 aactgtcgct gccgaaccaa ttgttcagga agcgccagct ccacgcacag aactggtgaa
    23821 agtcccgctg ccagtcgtag cgcaaactgc accagaacag caagaagaga acaatgctga
    23881 taaccgtgac aacggtggca tgccgcgtcg ttctcgccgc tcgcctcgtc acctgcgcgt
    23941 gagtggtcag cgtcgtcgtc gctatcgtga cgagcgttat ccaacccaat cgccaatgcc
    24001 gttgaccgta gcgtgcgcgt ctccggaact ggcctctggc aaagtctgga tccgctatcc
    24061 gattgtacgt ccacaagatg tacaggttga agagcagcgc gaacaggaag aagtacaagt
    24121 gcagccgatg gtgactgagc tccctgtcgc cgccgctgtc gaaccggttg ttagcgcgcc
    24181 agttgttgaa gaagtggctg aagtcgtaga agcccccgtt cagattgccg aaccgcaacc
    24241 ggaagtggtt gaaacgacgc atcctgaagt aattgccgcc gcggtaactg aacagccgca
    24301 ggtgattacc gagtctgatg ttgccgtagc ccaggaagtt gcagaacacg cagaaccggt
    24361 ggttgaaccg caggaagaga cggcagatat tgaagaagtt gccgaaactg ctgaggttgt
    24421 ggttgctgaa cctgaagttg ttgctcaacc tgccgcgcca gtcgtcgctg aagtcgcagc
    24481 agaagttgaa acggtaggcg cggttgaacc agagatcacc gttgagcata accacgctac
    24541 cgcgccaatg acgcgcgctc cggccccgga atatgttccg gaggcaccgc gtcacagtga
    24601 ctggcagcgc cctacttttg ccttcgaagg taaaggtgcc gcaggtggtc atacggcaac
    24661 gcatcatgcc tctgccgctc ctgcgcgtcc gcaacctgtt gagtaataat tagctcaagt
    24721 aatcaagccc tggtaactgc cagggctttt ttatttcatg tttgaatcaa catattaaaa
    24781 ttcataaaat ggtatccttg ctaaccaaca atgcaaaata ggcaaaaaaa atgcccgccc
    24841 cggcgatccc agagcaggca gacaaaacat acccagtttc atgatatgtt ttaaagcgag
    24901 cttatttgtt gagctggaag agcgacaatc cctgcatatc ggtaaatgct ttatacgatg
    24961 cctgcaatgc cgtttgctgc atgatgtaag atgaaatagt tgcattccag tccacatcaa
    25021 ccagatcgct catctgctgc gtttgcccta aagcgcgatc gctacctaat gaatccagcg
    25081 actccagttc gttcagctgc gtgcctaatt ccgcgcgaac ggtcaggaca ttgttcagcg
    25141 agtttttcag tccgcggttg gttttatcta acgccgcagc ggcggtttct ttatccgctt
    25201 cgctatccgc gaccggcgtt ttcagggctg cgatggcgct atccagcatg gcaaaaagat
    25261 tggtttcaga agcgctaccg tctggctccg caactgcgtt gctggtaata ctgtcgaaaa
    25321 ttttgtcacc cgtgtgccct atcaccatcg aacgcgaagc atcgacctgt tgcttaatac
    25381 tttctgctcc accgacgtat ttccctttct cttcgctaaa cggcgcagtc tctgttttat
    25441 aaccggcaaa aatgtagcgc ccattaccgt cagtggtgtt cgccagattc agcaactggt
    25501 cacgaagccc ctgaatatcc gtagccagcg aggcccggtc atcgtcactc aaggtgccat
    25561 tgctggcgta gacaattttt tcctgagcat tctggatagc ggtggtgacc tggctaagta
    25621 cactctcttc cagtgacact ttttgagtgg cgaaagtacg cgccagcgtg tactggctgt
    25681 tttgcgcctg tgcctgggag agaacaacag cttgtgatgc agcaatggga tcatctgaag
    25741 ggttaacgac tcgcttaccc gtcgacatct gttcgccgta cttcatccat tctgcctgag
    25801 aattggtgat gccgcgcatg ttttgctggt acatcatctg ggtactgaaa cgcattttat
    25861 ctccccttag cgaatgttaa tcagcgcatc aaaaatcgcg tttgccgtct gcagaacctg
    25921 cgcattcgcc aggtaatact gctgaaaacg ttgcagattt ccgtactcct catcgagatt
    25981 gacaccggaa atcgactgct gctgattgga aagctgcgtc accacattac cttgcgtggt
    26041 gctactggtt ttcaacgtcg cggttttatt accgatatca ctcactaacg aggcataagc
    26101 gtcgttaaag gatttcgcac cgcccaccgt tttactgttg ctttgcagat ccagcagggc
    26161 ctgaccgttg cggttgtcgc tatcacccgc atcttcttcg ctcgccatcg ctattttcgc
    26221 ttcgtcggtg attaatacat ccatgttgac gatggcgtca cttactggtt tcagcgtgaa
    26281 gctgtcgtta acggcaggcg ttcctgtaaa cgtcaactcc agaccatcaa atgccacttt
    26341 accgttggca tctggcgtca ccgtaaaagt ggtattgctg gcaaggcggg tgacctggca
    26401 ctgattatta tcgaacgaga ttttgtaatc agtcgccagt accgcggagg catcagttac
    26461 cgtggcaccg atcgcaacgt tacctttgtt tttcgtgtct tgcagaaccg cgggcttacc
    26521 gatagcaaag aaatcttcac ccgcatcgcc gttggcatca aatccagctt tgtgttggct
    26581 gttgaaagcc tcggcaaatg ccaccgccag ttgtccaagc gtattacgcg tctggtccag
    26641 atcctgagaa cggaatgtca gaatgccgcc cagcgacccg gtattcagta atttctccgg
    26701 gatctcaata ttgcctgccg tcccatcaac ataagcgaca gtcgtacgag aagggtcggc
    26761 gctggaagga actgccgcca gttgccgcgc cgtacttccc tgaaccagtg agtaaccatt
    26821 ggccatcgtg atgttataag tgccgccatc ctgaacgctg acttctacac caacaatctg
    26881 gtttaattcg ctcaccagtt gatcgcgttg atccagcaga ttgttaggtg acgcccctgc
    26941 ccccacgcct gtcaggcggg agatttgatc gttcaggctg gcgatttgtt tagcgtaatt
    27001 gttgatctga tcaacgctgg cacctatcgc gatattgacc tgtttgtcct ggtcgcgaag
    27061 atactgatcg gtggttttaa actgattcac caacccttct gatttcccaa tcagcgcctg
    27121 gcgcgctgcc gggtcttccg cgttactcac cagcgtttgc aggctggtga agaaatcctg
    27181 catctgtgtt gccagcgaag aggtactggt ggagagcata ttgtcgattt tcgacatctg
    27241 ctcatagcgg gcagtcaggc cgctactttg cgtctgcgcc gcacgtaact ggttggtaat
    27301 aaacgcatca tactcacgct gcacaccaga aacatagaca ccattaccaa cccagccgcc
    27361 agcgcccaac gtgctattgg cctgcgccat aatagtggtt tggcgggtat atccggcaac
    27421 gttatagctg gagatattat tacttgccgt atttaacgcc gcctgggccg cgttcagtcc
    27481 gctcatggcg ttattgatca agctggacat ggaggttcct tataagcctt caattacaga
    27541 gtattatcgg cagcgacccg ccggacttga gttattcaga acagattatc aatgttcatg
    27601 ctgtaggttt tgctcacctt gtcgcttatc gatttcatct gctgaatcat gttggtgagt
    27661 ttacgggcat agtgaggatc ggtggcatag cccgcgtcct gtagggcctg cgccccctgt
    27721 tccgcactcg cggcggtcgt cacggcagcg tagcgcgggt tacgtgttaa cagcccaacg
    27781 taatccgaca atgcttccag atacgagctg tagacccgaa atttcgcttt tactttcttc
    27841 gcttcgccat tttcatattc agtcgtggtg atttcagtaa ctggcccttt ccagttgcca
    27901 gaggctttga cgccaaacag gttatagctc ggctcgccgt tttcgcggcg gatttgccgt
    27961 tgcccccaac cagattccag cgccgcctga gcgaggatca aatgatgtgg cacaccgctt
    28021 tgctggcttg ccagttgggc gggcagcgag agttgcgcga ggaatgcttt actgtcaccc
    28081 ggcagcgaat catcgtagtt acgtggcacg gctttttgca ccagctgcga aagcgcctgg
    28141 ttttgataac gcaccacggt ttcgagcggg aatttcatcg gtgctgctga cgtgggctcc
    28201 tctggcaatg gttgttctgg cgtcatctgt ttaaccatca tctctgcaag ccccagacct
    28261 ttgcccgccg tcatctgttg ggcaatctgc tggtcataca tactggtata caggcgagtg
    28321 tgctcgctgc tgaacaggcc atcttttggt aaagcgtcgc gcatgctttt caacatcatc
    28381 tgcacgaaca tcccttccac ctgacgggcc accggacgga tatttgccgc cggatcttcg
    28441 ctcgctttcg cctttagttc gttgagtgat tgcgcatccc aggccgcact tgcctgtagt
    28501 ttgctgtcgc tgatcatcag atgatttcca gttttgcccg cagacatccc gcgctttgca
    28561 tagattgcag tatggacatt aaatccattg gggtggctcc caaggcgttg agcgcgcgca
    28621 ccacgttatt gaggctggcg ctggaacgta cgctttgcag cgaaccgccg ctctggcgta
    28681 agtcgatctg cgtttgtgga gtaaccacag tctgtccacc accaaacggt gtatctggct
    28741 ggctgacatt ggcctgacga ttgactgtaa ctgagagatt cccctgcgct accgcgcagc
    28801 tgtcgagggt cacttcgcga ttcatcacca ccgaaccggt gcgcgagtta atcactactt
    28861 tagcgtcctg cggggtgaca ttaacctgca tattctggat atcggcaagg aaacggacct
    28921 gggaactgtt gccacttggt acgcgcacct gaatagtccg cgcatccaac gcggtggcgc
    28981 tgccatatcc acgcacgcgg ttgatggtgt cagcgatttg ctgcgccatg ctgaaatctt
    29041 cgtcgttaag ttgcaaatta agggtattcc cgacgccaaa ctggctgggc aattcacgtt
    29101 caataaccgc accattggtg atccgtccac cgttcagttg gttcacctga acactgctac
    29161 cgccagcgga ggctcctgcg ccgccaacca gaatattgcc ctgcgccagc gcatacacct
    29221 gactgtcaac gcccttaagc ggtgtcatca acaacgtacc tccacgcaag cttttggcat
    29281 tacccatgga agaaaccacc acatcgatgg tttgcccctg acgtccaaac ggaggaagtg
    29341 acgctgtcac cattaccgca gcgacgtttt ttagctgcat attggtgccc gtcggaacgg
    29401 taattcccag ctgtgagagc atgttattaa gcgtttgtgt ggtaaacggc gtctgggttg
    29461 tctggtcacc ggtgccatcc agccccacca ccagaccata gccaatcagt gagttttgcc
    29521 ttaccccctg aacactggtg agatcgcgaa tacgctcagc ctgagccgcc gtcgtaacca
    29581 atagaagaat taatgcagag agaaatttaa tcaccacagc ctcacttaca ttggcgacag
    29641 gttaaggaag aaacgttgca accagcccat attttgcgct tcgttaatgt agccattgcc
    29701 tacgtattca atgcgcgcat ccgccacctg agtagacggt acggtattgc tgccgctgat
    29761 ggtgcgtgga ttaaccacgc cagagaagcg aataaattcg gtaccctgat taatggcaat
    29821 ctgtttttca cccaccacat gcaggttgcc gttgaccagt acctggtcaa ccgtcaccgt
    29881 caacgtgccg ctaaaggtat tgctggcatt ggccccgccc tttccgttga acgtgttacc
    29941 accggaggct tcgacatcgg cacgagcgtt accaaacagc ccctgcaaat agcgcggcac
    30001 agtatcaaag ccaaaattag ttttaccgtc acggctggca ttcgcagagg agcttttgct
    30061 ggcgctgacg ttctcctgca acacgatggt cagcgtatcg ccaatattgc gtggtcgacg
    30121 atcttcaaac agcggttgat agccatagtt aatcggctga gcagactgga aaatagaacc
    30181 gttggcgaca ggcgtcggac cgggaaccgg ttgtgcactg gtcgccccct gcaccagcgg
    30241 cgtggagggt atccaggcgc agccggttag tgaaagcacc aacaagctgg aaatggcata
    30301 agtatgcgca gcgttttttt gcatggctat catcttcaaa aatcaataaa ccggtgaacc
    30361 tgccaccggt tatgccttag agttgcgtca gtttttgcag catctgatcg gtggtggaca
    30421 ccgctttact gttgatttcg taagcgcgtt gcacctgaat catattgacc agttcttccg
    30481 ctacattgac gttagatgtt tcaacatacc cttgatacag cagtcccgcg ccgttcaggc
    30541 ccggcgtgct ttcgttcggt gcaccggagg attgcgtttc ggtgtagagg ttttcgccaa
    30601 tgctctccag cccggtgtca ttcataaagg tggtgagatt gagctgccca acctgaaccg
    30661 gagctgcctg gccttgttgg gttacgctga ccacgccatc acgaccgatg gtgatactta
    30721 acgcattcgc cggaatggtg atcgctggct gcacctgaaa accaccagcc gtcaccagct
    30781 gcccgttctg atccacctgg aaagagccgt cacgggtata ggctgatgaa ccatctggca
    30841 acatcacctg gaaaaagccc tgccctttaa tcgcgacatc tttgctgttg ttggtctgcg
    30901 acaggttgcc ctggctgtgt aagcgttcag tggcgaccgg gcgtaccccc gtgccgattt
    30961 gtaatccgga gggtaaggtg gtttgttcgg aagactgtgc ccccggctgg cgaatggttt
    31021 gataaagcag atcttcaaac accgcgcgct gacgcttaaa accgttagta ctgacgtttg
    31081 ccaggttgtt ggcaatgacg tccatattgg tttgctgggc gtcaaggccc gttttggcga
    31141 tccataatga actgatcata aaatatcctg tagtggatta gtgacaaagt gcatatgtct
    31201 tcttgccgga tgcggcgtaa acgccttatc cggcctacaa gtgctcgcaa aatcgataaa
    31261 ttgcgattgc ccccatagac ccgataaact tgcgcatcgg gcaacttata cttgtcatgt
    31321 atcctttcaa ttaactcatc gacagcagtt ggttggcacg gcctgcgtta tcatcgacgc
    31381 tgctgatcac cttcatctgc atttcaaaac gccgcgcgct ggcaatcatg tcgctcattg
    31441 ccgcaacggc attgacgtta ctgccttcca gaacacccga catcacacgc aaggttggat
    31501 ctgcctgcag taccggtcca cgtgtagcct gagtttctgc gcttaaacga aaaatgccgt
    31561 cgtcaccgcg ctgcacttcg ctgcccgtgg ctttcaccag cttcagacgc cccactggcg
    31621 caaccgtatt tgccggatcg cccggattca gcgccgagat tgtgccatcg gcagcaatcg
    31681 tgatttccgc cccttccggc acagcaattg gccccgcctc gcctatcacc ggatgcccct
    31741 gaattgtcag ttgcccggtg ggatcaacct gaatgctgcc attacgcgta tacccttcgc
    31801 tgccgtcagc ggtctgcacg gccagccagc catcctgctg caatgcaacg tccagcgggc
    31861 gcgaggtgta atccatcttg ccgggcgtca tgtctgcacc cggtgttgac gccgttacca
    31921 gcgtgcgcgt gggcagtgaa agcccttcca ccggcactgc gcgcaaggcg tttaactgcg
    31981 cgcgaaaacc gggcgttgag gcattggcca gattactggc ggttaccgcc tgttgattca
    32041 gcgtctggct ggctgcaccc atcgcagtat aaattgcgtg atccattgag ctatcccgtc
    32101 agcgattagc gtaagttaac cagcgtgttg aggatctggt cctgggtttt gatggtctgg
    32161 gcgttagact gatagttacg ctgggcaacg atcatattga ccagttcttt actgagatcg
    32221 acgttggacg cttccagcgc accgttggtc agggtgccaa agtttcccgt cccggctgtc
    32281 cccaacagcg ccacgccaga agattgcgtc gcagaccaga cgttgtcgcc ttcggatgcc
    32341 agaccttcgt tgttggcaaa gttcgccagt acaatctgcc ccagcagttg ggtttgttca
    32401 ttggaatagt tgccgacaac cgtaccgtca tcattgattt gataactcac cagatcgccc
    32461 ggtttgtagc cattctgggt ggttgccaca atattgttag cgccggtatt ttgctgcatg
    32521 gagttgagga agctcaggct aaacgtggcg ggatctgcgc cgttaattgc gccagtggta
    32581 atattctctg ttggatttga cgtcagaacg ccattggcat taaacaccag cttcattgca
    32641 ggctcggctg tacctgttgg atcactgcta tcctgggtgt agacctgcca gttattatcc
    32701 ccggtcttca caaagtagac gctcatgtca tgagcattac cctgactgtc gaaaacagtc
    32761 accgaacctt ttttgttata gctatccgca ttgctggcat caaatgcgtt aacagtagga
    32821 agcggatcac tggaattcag gttgatctgc atcgacgccg tggtggtagt tttcgctgcc
    32881 atcagggtat tcgggatcga aatattggtc ggattcgcac cttgctgaat agtcggcggc
    32941 gtaccggttg ccgggtaacc cgtcagctgt aaaccttgca tattcaccag gttacggttt
    33001 tcatccagct taaattgtcc gttacggctg tagaacaccg aaccgttgct gtctaccaga
    33061 cggaaaaaac cgttctggct gatagcaacg tccagaccac gcccggtgtt ggtggtcgtg
    33121 ccatcggtaa agtcctgagt gataccggca acttttaccc ccagtcccac tttcgaaccg
    33181 gcaaacatat cggcaaaaga ggccgtgcct gatttaaagc cgtaggtggc ggagttggcg
    33241 atattgttgc caataacatc gaggttggtg gcggcagcgt ttaatccgct aaccgcttga
    33301 gaaaaggcca tgactgactc ctgaaatgtg aaggcttaaa ttatctgccg tacttcgtcg
    33361 agggtggtgg tgccgtaagt gccgagatcc agcgtattac cgttgttgcc gcggatcaca
    33421 ccctgcacca gagcaaactg cagcggctgg gcaaccagtt gtgtaccacc gttactggcg
    33481 ctaatcgcta cgttgtaaga accgttcggc gcagtgctgc cgtcagtcaa cgtaccgtcc
    33541 caggtgaaac tgtggactcc ggcggtcagt tcaccaatat caatggtgcg cacaaccgcg
    33601 ccatttttat cggtgatggt ggccgtaact ttgtctgccg cctgttgcag ctcaacacca
    33661 aacggcgtgg tcgtggtcac agccccttct tcactgccgg ttcccgcaag aacagtggtg
    33721 ccggggatca tcacgccgtg accgatcagg ttactggcct gtaacgactg gctgttatca
    33781 atctgtccgg aaatagatcc gagcgtggta ttgagttttt caatcccgct gaccgtgctg
    33841 atttgtgcca attgcgacgt cagctcgttg ttttccattg gattggtcgg gtcctggttt
    33901 ttcagctgcg ccaccaacaa agtcagaaaa ctgctttgta aatctgcggc gttgctgccc
    33961 gtgagcgaac tactgctggt ggtactgacg ccggtatttg tcggatcggt ggtggttacc
    34021 gcaatggaca tagctttctc ctttattgac cgagcgtaag ggttttcagc atcatgcttt
    34081 tcaccgtgtt gagcacttca acattggcct gatagctgcg tgacgccgac atggtgttaa
    34141 ccatctctcc gacaacatca acgttcggca tttttacgta gccctttgca tccgccagcg
    34201 gattacccgg ttcataaacc agtttgtccg gggcctgact ttctataaca tcggcaacct
    34261 ttacgccgcc tgtcgcagca cctggtgcag cgttaacctg gaataccacc tgttttgccc
    34321 gatatggctg tccatcggga ccggtcacgc tatcagcatt tgccagatta ctggccgcca
    34381 cgttcaggcg ctgggactgg gcagttaacg ccgacccggc gatatcaaaa atattcagca
    34441 gtgccatccg ttaatttccg ctctgtaaaa cgttcatcat gcctttgatt tgcccgctca
    34501 acgcactaag gctcatctgg tattgcaggc tgttatcggc aaactgggtg cgttcgcgat
    34561 ccatatcgac ggtattaccg tcaagcgaag gctggtccgg aatgcggtat tgcagttctg
    34621 cggtaggagg cgtcagcgcc tgcgccggaa tgtgttgcgt tgaggtcatc gtcagagcaa
    34681 ccacactggt tgcatcccgt ccacgttgca tgactttttt aagttcactg gcaaaatcga
    34741 tatcgcgcgc ctgataacca ggggtatcgg cattggcgat gtttgctgcc agcacttcct
    34801 gacgctgggc gcgcagattg agcgcctctt gttgaaaacg taaggcggcg tcgagcttat
    34861 cgagcatatc tcctccgcag gtatcaaaat tctgccatca gcttaaatgc ctttacgcgc
    34921 gcgttatcgg cggaataaac gcaaaatggg tcgctattta tgccgttgat gaccattggg
    34981 gacgggtaca attcacgttg tagaaatagc tggggggcga aaatgctggc aataaaacgt
    35041 agcgtggcga tcatcgcgat actgttcagt ccgttaagtg cggcgagcaa tctcacatcg
    35101 caattgcaca ccttttttag cgcccagctc gtgggggtaa gtgatgaggt tcgtgtttct
    35161 attcgtacag cacccaatct gctaccgccg tgcgagcagc cattgctttc gatgagcaat
    35221 aattcccgcc tgtggggcaa tgtgaatgtg ttggcacgct gcggtaacga caaacgatat
    35281 ttacaggtta atgtacaggc cacaggaaat tatgtggttg ccgcgatgcc cattgtacgt
    35341 ggaggaaagc tggaagctgg caatgtcaaa ctgaaacgcg gacggctgga taccttgcca
    35401 ccgcgtacgg tgctggatat caatcaactt gttgatgccg ttagcctgcg cgatctatca
    35461 cccgatcaac ctatccagtt aacccagttt cgccaggcat ggcgggtaaa agcgggacaa
    35521 cgcgtcaacg tgatcgccag cggtgatggg tttagcgcca acgcagaagg tcaggcgctg
    35581 aacaatgcag ccgtcgcaca gaatgcacgg gtgcgcatgg taactggaca ggtagtcagc
    35641 ggcgttgttg atgcagatgg gaatattctt ataaacctgt aagctgttaa agattacccg
    35701 tcccttgccg ataaataagc aacacatgat aaaagcgccc tcaatgagga ataaaccatg
    35761 agtattgatc gcacttcgcc tctgaatccc gtaagcaccg ttcaaccgcg cgaaaccact
    35821 gacgcgccgg taacgaacac ccgggcggca aaaacaacag cctccaccag caccagtgtg
    35881 acgttaagcg acgcgcaagc aaaactgatg caacccggca gcagtgatat caatcttgaa
    35941 cgtgtcgaag cgttaaaact ggcgattcgt aacggtgaac taaaaatgaa caccggcaaa
    36001 attgccgatg cgctgatcaa cgaagcgcag caagacttgc agagtaatta atcgtatgac
    36061 gcgtcttgca gagatcctcg accagatgtc cgctgtgctt aacgatctca aaacggtaat
    36121 ggatcaagag cagcaacatc tctctatggg gcagatcaac ggcagccagt tgcaatggat
    36181 tacagaacaa aaaagctcac tgctggcaac gttggattac ctcgaacagt tacgcaggaa
    36241 agaacccaat acagcaaata gcgttgatat tagtcaacgc tggcaggtaa ttgctgtgaa
    36301 aacgcagcaa ctacgccaaa tgaatcaaca taacggctgg ttactggaag gacagattga
    36361 gcgcaatcaa caggcgctgg aaatgttgaa accgcatcag gaaccgacgc tatatggggc
    36421 gaacggtcag acctcaacaa cccatcgcgg cggtaaaaag atttcgatct gaagattatc
    36481 tccggcctgc actgcaggcc ggaatgcatt gttacaccgt ccggcgggca aattctttaa
    36541 ctttgaagcc cagtaccgcc agtgcagcga agtacgcggc aatccccgcc agcacgaccg
    36601 ccattaaacg cagcaagcgc cagggcatgg tacccaatga ccactccggc atgatatgta
    36661 acatacctaa aagcacgcca gacatcacca gtaccgccac caccagacgc aacagaaacg
    36721 ccatccagcc aggttgcggg gtaaagattt tctgcttacg caactgccag taaagcagcg
    36781 aagcattcag acacgccgcc agaccaatag aaagtgacag cccggcatgt ttcaacggac
    36841 caataaacgc caggttcatc aattgcgtta aaatcagcgt aacgatggca attttcactg
    36901 gcgttttaat gtcctggcgg gaataaaagc caggagccaa cactttcact acaatcaggc
    36961 caatcaaacc caccgagtag gcaattaacg cccgctgggt catcagcgca tcaaacgcgg
    37021 taaatttacc gtactggaac agcgaaacgg tcaacggacc tgaaagaatg cccaacgcaa
    37081 ccgcactcgg cagcgccaac aggaaacaaa gacgcagccc ccagtccatc aaacggttgt
    37141 attcatcatg attgccactg gcaaaacttt tcgacagcga cggcagcaaa atggtgccaa
    37201 gcgccacgcc cagcacaccg gacggaaact ccattaagcg gtcggcgtaa tacatccacg
    37261 acaccgaacc ggaagcaaga aacgaggcaa aaatggtgtt gatgattaag gagatctggc
    37321 tcacagagac accaaggatt gccggtccca tctgtttcac cacacgcatt gctccggcat
    37381 cgtggaagtt aatgcgtggc aaaaccagca tgccgatctt cttcaggtgc ggtagctgat
    37441 acaccagctg caggatgccg ccgaccgtaa cagcccacgc cagcgccagc accggtgggt
    37501 taaagtacgg tgcggcaaac agcgcgaaac caatcatgct gatgttaagc agtgttggag
    37561 caaacgccgg aatcgagaag cggttccacg tattcagaat cgctcccacc agcgacgcca
    37621 gggagatcag caagatatag ggaaaggtaa tctttagtag ctggctggtc agggcaaatt
    37681 tgtcagctgt gtcagcgaag cctggcgcgg tcaccatgat cacccacggt gcggcgagca
    37741 tgccagcgac cgtcacaacc gccagcgcga gtgtcagcag gccagaaaca taagagacaa
    37801 agacccgcgt ggcatcttca ccctgcttac ttttatattc cgccagaatc ggtacaaatg
    37861 cctgggaaaa tgccccttcg gcaaagatac ggcgtaacaa gttaggaagt ttaaaagcga
    37921 cgaaaaaggc gtcggttgcc atccctgcgc caaagattct ggcgacaatt gcgtcacgtg
    37981 cgaagccaag cacacgcgaa aacatggtca tcgagctgac ggcggccagc gattttaata
    38041 aattcatcgg tgttctaatc cagacccacg ggcaaaacgc ctgcaaggca ggcgctgaaa
    38101 gaggcgaata gtctacctgg attatggtga attgctaccg ccagatgtta cagggtttat
    38161 tcactcatcg catcgcgcca gatcttgtca acgatacgtt gcgccagcac tgcctgttcg
    38221 ccggcggttt gcggaactgt ctgattttgc acacattcaa tgaagtgccg cgcacagccg
    38281 acaaatccac gttgctcaag cgtgctctgc caaccaggaa tcggtttatt cactacaccc
    38341 tgcccacgct cctcacgcca ttcgcgcatt tccgtaatgt cgatgagcgc cccgtcagtc
    38401 acggcctgca cggtttcacg ctgacttccg gcacggcgat gcatacaagt ggtgatctgc
    38461 aaaggaccgg ccgaaaaatg gtgctcggca aacagcattt cgccagcgtc gttagtcaat
    38521 agcgtaccgc catccagaga ggctttgccg cccgacaacc acagcgcggt atccaccaca
    38581 tgcagataat catccagcaa cgtgaaataa agatcgtttg gcccgacgct attgctacga
    38641 tgtttatcca ttcttagcga ggctgcggtg gcgagttgcg tttttaactc accgtagagt
    38701 ggtgcgaaac gacggttaaa accgaccatc aacgtcagtt ttttccgcgc cgccagttcc
    38761 accagccgtt cagcatcgcg cagattttct gccagcggtt tatcgacaca gacatgtacc
    38821 cccgcattga gtaacgtact gaccacgtca aagtgactgg cggtgctgga atgtacaaaa
    38881 accgcatcgc aactggcggc aaggctggat aacgaatcgg cataaggaat gcgccagctt
    38941 tcacaaattg gcagggcttt cgcgcgcgta ggcgaccagg ctccttgtaa cgtccagtca
    39001 gacgctgccg ccagcaccgg taaccacgct ttttgcgcaa tgccgcctaa tcccactacg
    39061 ccgatacgta attttttcac tttaatctcc caggtgggcc agcaacgaat caagacgctg
    39121 tttcagttct gccacttcga tttccagggc ttcaacgcgg gcctgtaaat caccgtcaac
    39181 ggcgctcgac atatccgtta ccgacggctg atcctctacc tcaccgctga aaagatgcat
    39241 gtagcggctt tcgcgtttac ccggttcgcg ggccagacgc accacaaaag gaccatcttc
    39301 gcgattcgcc agttgttcca gcgtcaactc cacttccgcc atatcgctga attcatacat
    39361 tcgcgcggcg cggctgcgca gttcacccgg cgtctgggca ccacgcaata acaacgtggt
    39421 gattaacgcc acttctgcgg cgctcagttt cagatcgcca aattctgaat tacaaaaacg
    39481 ttgctcatat ttggtgactc gattaccaaa gccactcacc gtgcgcagat aatgacgttt
    39541 gaccagatta tccagctgtt cctgcacttc agattcactc agattcatga ccggttcacg
    39601 gttcgttttc tgattacagg ccgtgactac accattgact gagagcggat attgctccgg
    39661 cgtggtcacc tgcttttcca gcaagcagcc aatcacgcgg gcttccagag cagtaagttg
    39721 atatttcatg agattctcct tagcggccgg gcgtccagtc tggggtagtt aatgccgtca
    39781 gtacgtgatc gcgccattgt ccatcaatca acagatagtc tttcgcatag ccttcttttt
    39841 caaaacccag tcgcgccagt aaatcaccgc tacgtttatt gtgcggcata taattagcca
    39901 taatgcgatg aatatgttgg gtgcgctgca tataacgaat ggctgcggtc agggcttcaa
    39961 acatgagtcc tttgccctgc catttttgcc caatcgaata accgagatag caggcatgaa
    40021 aagagccgcg aacaacattg gaaaaattgg caacgccaat aatctctttt tcgtccgggt
    40081 cgaataagcc aaagtagaaa gctgaacctt gtttatgaaa ttcgttaatc atccccagcc
    40141 tggcctgcca gcctgatgga taacagtggc tttcgtctcg cactggctcc cagggcttga
    40201 ggaaatggcg attctctgcg taataatccg caagacgcca ggcatcacga tcatgcacca
    40261 gacgcacgac cagtcggtct gtggttaagc gcacttttgg cacgttactg cgatagccaa
    40321 acattctata cctactcctt cccgtaacgt ctttaatacg ctgtaagtat tactatacct
    40381 gcgcaaccag accctgtgaa aagcccaaaa tgccattttc tgcattattc acatatttga
    40441 taaaaactct ctatcaaagc gacgttttga taaaaaaata ttgtccccga agtgatggga
    40501 aaagtcttag cggcaatgca gaatagatag tgctcatctt ttatttcccg ggaggggaaa
    40561 tgtcccgcgt gtcgcaggcg aggaacctgg gtaaatattt cctgctcatc gataatatgc
    40621 tggtcgtgct ggggttcttt gttgtcttcc cgctgatctc tatccgcttc gttgatcaaa
    40681 tgggctgggc cgccgtcatg gtcggtattg ctctcggtct acgccagttt attcagcaag
    40741 gtctgggtat tttcggcggc gcaattgccg accgctttgg tgccaaaccg atgattgtta
    40801 ccggtatgct gatgcgcgcc gccggattcg ccacaatggg tatcgcccac gaaccgtggc
    40861 tattgtggtt ttcatgcctg ctctcgggac tcggcggcac gttgtttgat ccgccgcgtt
    40921 cggcgctggt ggtgaaacta atccgtccac aacaacgtgg tcgttttttc tcgctgttga
    40981 tgatgcagga cagtgccggt gcggtcattg gcgcattgtt ggggagctgg ctgttgcaat
    41041 acgactttcg cctggtctgc gccacagggg cagttctatt tgtgctgtgt gcggcgttca
    41101 atgcgtggtt gttaccggca tggaaactct ccaccgtacg cacgcccgtt cgtgaaggca
    41161 tgacccgcgt gatgcgtgac aagcgttttg tcacctatgt tctgacgctg gcgggttact
    41221 acatgctggc tgtacaagtg atgctgatgc tgccaattat ggtcaacgac gtggctggcg
    41281 cgccctctgc cgttaaatgg atgtatgcca ttgaagcgtg tctgtcgtta acgttgctct
    41341 accctatcgc ccgctggagt gaaaagcatt ttcgtctgga acaccggttg atggctgggc
    41401 tgttgataat gtcattaagc atgatgccgg tgggcatggt cagcggcctg caacaacttt
    41461 tcaccctgat ttgtctgttt tatatcgggt cgatcattgc cgagcctgcg cgtgaaacct
    41521 taagtgcttc gctggcggac gcaagagctc gcggcagcta tatggggttt agccgtctgg
    41581 gtctggcgat tggtggcgct attggttata tcggtggcgg ctggctgttt gacctgggca
    41641 aatcggcgca ccaaccagag cttccgtgga tgatgctggg cattattggc atcttcactt
    41701 tccttgcgct gggctggcag tttagccaga aacgcaccgc gcgtcgtttg cttgaacgcg
    41761 acgcctgatt tgatactccc ccctgactcc tccatactga aagtgaactg acagccaaat
    41821 ggaggagtca cgtgaagcta tacatttacg atcactgccc ttactgcctc aaagcccgca
    41881 tgattttcgg cctgaaaaat attcccgtcg aattacatgt tctgctcaac gacgatgcag
    41941 aaacacccac ccggatggtc ggtcaaaaac aggttcccat tctgcaaaaa gatgacagcc
    42001 gctatatgcc agaaagcatg gacatcgttc actatgtcga taaactcgac ggcaaaccgt
    42061 tactgaccgg caaacgttcc cctgccattg aagagtggct gcgcaaggtc aatggctacg
    42121 ccaacaaact gctgttgccg cgttttgcca aatcggcatt tgatgagttt tctactcccg
    42181 ccgcgcgcaa atatttcgtc gacaagaaag aggccagcgc gggtaatttt gccgacctgc
    42241 tggcccactc tgacggtctg attaagaatg tcagcgatga tttacgtgcg ctggacaaac
    42301 tgatcgtcaa accgaacgcc gtgaatggcg aactttcgga agatgatatt cagctatttc
    42361 cgctactgcg taatctgacg ctggtagccg gaatcaactg gccaagccgc gttgctgatt
    42421 accgcgataa tatggcgaaa cagacacaaa tcaatttgtt atcatcaatg gcgatttaat
    42481 cctgacccgc tgcggcagcc tgtattcgcg ggttgccgcc gttttcagtt ttttctgctg
    42541 gcgagtttat cgctggtgac acatgctgcg ttgtgtcagg atattgacgc agatttcatg
    42601 tcgaggacta ccatgaacaa gtttttattt gccgctgcat tgatcgtcag tggcctgctc
    42661 gttggctgta atcaactcac ccaatacacc atcaccgaac aagaaattaa ccagtcgctt
    42721 gcgaaacata ataatttctc aaaagatatc ggtttacccg gtgtggctga cgcccatatt
    42781 gttctgacaa acctgaccag ccaaattggt cgcgaagagc cgaataaggt taccctaacc
    42841 ggagacgcca atctggacat gaactccctg ttcggtagcc agaaagcgac catgaaactg
    42901 aagctgaaag cgctgcccgt gtttgataaa gaaaaaggcg cgatcttcct gaaagagatg
    42961 gaagtggtcg atgcgacggt acaaccggaa aaaatgcaaa cggtgatgca aacgttgctt
    43021 ccctatttga accaggcatt acgcaattac tttaaccagc aacctgctta cgtcctgcgc
    43081 gaagatggca gccagggcga agcaatggcg aaaaaactgg cgaaaggcat tgaagtgaag
    43141 ccaggcgaaa ttgtcattcc atttactgat taatcacgag ggcgcattcg cgccctttat
    43201 ttttcgtgca aaggaaaacg tttccgctta tcctttgtgt ccggcaaaaa catcccttca
    43261 gccggagcat agagattaat gactgcacca tcccaggtat taaagatccg ccgcccagac
    43321 gactggcacc ttcacctccg cgatggcgac atgttaaaaa ctgtcgtgcc gtataccagc
    43381 gaaatttatg gacgggctat cgtaatgccc aatctggctc cgcccgtgac caccgttgag
    43441 gctgccgtgg cgtatcgcca acgtattctt gacgccgtac ctgccgggca cgatttcacc
    43501 ccattgatga cctgttattt aacagattcg ctggatccta atgagctgga gcgcggattt
    43561 aacgaaggcg tgttcaccgc tgcaaaactt tacccggcaa acgcaaccac taactccagc
    43621 cacggcgtga cgtcaattga cgcaatcatg ccggtacttg agcgcatgga aaaaatcggt
    43681 atgccgctac tggtgcatgg tgaagtgaca catgcagata tcgacatttt tgatcgtgaa
    43741 gcgcgcttta tagaaagcgt gatggaacct ctgcgccagc gcctgactgc gctgaaagtc
    43801 gtttttgagc acatcaccac caaagatgct gccgactatg tccgtgacgg aaatgaacgg
    43861 ctggctgcca ccatcactcc gcagcatctg atgtttaacc gcaaccatat gctggttgga
    43921 ggcgtgcgtc cgcacctgta ttgtctaccc atcctcaaac gcaatattca ccaacaggca
    43981 ttgcgtgaac tggtcgccag cggttttaat cgagtattcc tcggtacgga ttctgcgcca
    44041 catgcacgtc atcgcaaaga gagcagttgc ggctgcgcgg gctgcttcaa cgccccaacc
    44101 gcgctgggca gttacgctac cgtctttgaa gagatgaatg ctctgcagca ctttgaagca
    44161 ttctgttctg taaacggccc gcaattctat gggttgccgg tcaacgacac attcatcgaa
    44221 ctggtacgtg aagagcatca ggttgctgaa agcatcgcac tgactgatga cacgctggtg
    44281 ccattcctcg ccggggaaac ggtacgctgg tccgttaaac aataaaaatc gtagcttcct
    44341 gttgtcatta ggttatttta cctgtataaa taaccagtat attcaacagg gggctattat
    44401 gcgaattgaa gtcaccatag cgaaaacttc tccattgcca gctggggcta ttgacgccct
    44461 ggctggcgaa ctttctcgcc gtattcagta tgcgtttcct gataatgaag gccacgtatc
    44521 ggtacgttat gccgcagcga ataatttatc ggttattggc gcgacaaaag aagataaaca
    44581 gcgcattagc gaaattctcc aggaaacgtg ggaaagcgcc gatgactggt ttgtcagcga
    44641 ataatatgca gtgatttttg tttttgccgg gtcgccccgg cttttttatt ttacttacga
    44701 attttgagta caaaatttct acacctcctc aaaataattg cacaatatgc taaaaaaata
    44761 tgaatgacat gctgttttat tgttcatata aatcttaaaa gcagaaattt atctgtttcc
    44821 ccctttaaaa attcggtgaa tacccttact tattggtata ctgaagttgc ttcagataaa
    44881 aacgcattga acctcgaata acgttgtcta gtaacacgaa ttagggggcc atgatggaaa
    44941 aaaataatga agtcattcag actcatccgc tcgtagggtg ggacatcagc accgttgata
    45001 gctatgatgc gctgatgttg cgtttgcact accagacccc aaataagtcc gagcaggaag
    45061 ggactgaagt tggtcagacg ctctggttaa ccactgatgt tgccagacaa tttatttcga
    45121 tattagaagc aggaatcgcc aaaattgaat ccggtgattt cccggtaaac gagtatcggc
    45181 gtcattaaca tgacatgacc atccacccaa aaggcacctc accggtgcct ttaccatttc
    45241 tgggttgtat atctgcattc agttaattac tctgcttaaa ccatgagagc agagagaaaa
    45301 aaatgaaata cgatctcatc attattggca gcggttccgt aggcgctgcc gccgggtatt
    45361 atgcaacccg cgccggttta aacgtgctta tgaccgacgc ccatatgcca ccacatcaac
    45421 acggcagcca ccacggcgat acgcgattaa ttcgccatgc ttatggtgaa ggcgaaaagt
    45481 atgtcccgct ggtcctccgc gcgcaaacgc tgtgggatga gctctcccgc cacaacgaag
    45541 atgatcctat ttttgtacgc tctggtgtca ttaaccttgg cccggctgac tccgcatttc
    45601 tcgccaacgt cgcccacagc gccgaacagt ggcaactcaa cgttgaaaag ctcgatgcgc
    45661 aagggattat ggcccgctgg ccagaaatac tcgtcccgga caactacatc ggcttatttg
    45721 agactgattc cggttttttg cgcagcgaac tggcgattaa aacctggatc caactggcga
    45781 aggaagcggg ctgtgcgcaa ctgttcaact gcccggtcac cgcaattcgt catgacgatg
    45841 atggcgtaac tattgaaacg gctgacggtg agtatcaggc gaaaaaagcg attgtctgcg
    45901 cgggaacatg ggtaaaagac ctgctcccgg agctgcctgt ccagcccgta cgtaaagtat
    45961 ttgcctggta tcaggccgat ggccgctata gcgtgaagaa taaattcccg gcgtttaccg
    46021 gtgaactgcc caatggcgat caatattatg gttttccggc agaaaacgac gcgttgaaga
    46081 ttggtaaaca taacggaggc caggttatcc attcagcgga tgaacgtgtt ccgtttgcgg
    46141 aagtggccag tgatgggtca gaagccttcc cgttcttgcg caatgtattg ccgggtatcg
    46201 gttgctgcct gtacggcgct gcctgcacct atgataattc gcctgacgaa gattttatta
    46261 tcgataccct acccggccac gataatacac tgctcattac cggcctgagt gggcacggtt
    46321 ttaaatttgc gtcagtttta ggggaaatag ctgccgattt tgcgcaagac aaaaaaagcg
    46381 attttgattt gacgccattc aggctttccc gcttccaata atcatcatta cggcctccag
    46441 aatgaggccg tttttacagg agtatttaat gcgtcggctg ttgcactatc tcatcaataa
    46501 tattcgcgag catctgatgc tttatctttt tctctgggga ttgttggcaa ttatggatct
    46561 gatctatgtg ttttattttt agttttccgc actgacataa actgacaaat atttaatctc
    46621 cctttcattt agcgtgaaaa attgaatgag attattcctg cttctacagc actcaccaga
    46681 catctctgat gctgaaccca ctaaacttat gaagattctt tcattattaa ttcaaaatta
    46741 ttgaaataaa aataaatatc attcaatttg ttatttttgt gttgcaattc attctccgac
    46801 acgccatttt ctttctctcg caaattcgtt ttggatgcac ttatgtcatt cacaaatacc
    46861 cctgaacgct atggtgttat atctgcggcc ttccattggc taagcgcaat tattgtctac
    46921 ggcatgtttg ctttaggtct gtggatggtc acgctcagtt attacgatgg ctggtatcac
    46981 aaagcacccg aactgcataa aagcatcggt attttgttaa tgatggggct ggttattcgc
    47041 gttctgtggc gtgtcatatc tccaccgccg ggtccgctgc cgagttattc gcccatgact
    47101 cgtcttgccg caaaagctgg acatctcgca ttgtacctat tgctgttcgc catcggcatc
    47161 agcggctatc tgatctcaac tgccgatggt aaaccgatca gcgtttttgg ctggtttgac
    47221 gtccccgcga cccttgccga cgctggcgca caggcagact ttgccggtgc cctgcatttt
    47281 tggctcgcgt ggagcgtcgt agtactgtcc gttatgcacg gatttatggc cctgaagcat
    47341 catttcatcg ataaagacga cactctgaag cgcatgctgg gaaagtcgtc atctgactat
    47401 ggagtataaa aaatgaaaaa aagcctgctt ggtttaacct tcgcgtccct gatgttctct
    47461 gccggttcag cggttgccgc cgattacaaa attgacaaag aaggtcagca cgcctttgtt
    47521 aacttccgca tccagcatct tggctatagc tggttatatg gcacctttaa agatttcgac
    47581 ggtactttta cctttgatga aaaaaatcca gctgccgata aagtgaatgt gacaattaac
    47641 accaccagcg tcgatactaa tcacgccgaa cgtgataaac atcttcgcag tgcagatttc
    47701 ctcaataccg caaaatatcc acaggcaaca ttcacctcca ccagcgtgaa gaaagacggt
    47761 gacgaactgg atattaccgg cgatctgacg ctgaatggcg taaccaaacc cgtcacgctg
    47821 gaagcgaaat taattggtca gggcgacgac ccgtggggtg gtaaacgagc aggctttgag
    47881 gccgaaggca aaattaagct caaggacttc aatatcaaga cagatttagg tccagcttct
    47941 caggaagtgg atctgattat ttcagtggaa ggcgtacagc agaagtaaga gtgaaatgtt
    48001 gccggatggc acgcatccgg caatgatatt tattctgttg gatcaggaat gcccagtgtt
    48061 gtattcagac gtccacgaga cttattaaag atcttattgc cattttcacg tcctgcccga
    48121 cggcgtcgct gttcttctgg cggtaacgcg ctttcttcgc agcaaatctc actacaacaa
    48181 cctttgtatt tttccgcaca tactggacac tgaataaaca gcaggtggca gccatcattt
    48241 ttacagttgg tatggctgtc gcacggcgca ccgcactggt ggcaatgcgc aataatctca
    48301 tcagttatac gttcgcccat ccgctcgtca aaaacaaaat ttttgccaat aaaacgcacc
    48361 ggcaagccct gctcgcgcgc cctacgggcg tattcaataa ttccgccctc gatatgccag
    48421 actttattga atccgttatg tttcatccag gcactggcct tttcacaacg aatgccgccg
    48481 gtgcagtaca tgacgatttt tttatcttta tgtgcctgca tcatctcgac tgcttttggc
    48541 agctgctcac ggaaggtatc tgctggaatt tccagcgcgt tttcaaagtg ccccacttca
    48601 tactcatagt ggttacgcat gtcgataaac agtgcatcgg gatcgtcaag catggcgttc
    48661 acttccgccg cttgcagata ctcaccaaca ttgctggcat caaagtgagg atcgtcaata
    48721 ccgtcggcaa cgatacgatc gcggaccttc atgcgcagta cccagaagga tttcccgtca
    48781 tcatccaacg cgatattcag gcgtaagcca tctaaagccg agtcgaaggc atagagctgc
    48841 gcgcgaaatg tttcaacatt gctcgcaggt acgctgattt gcgcgttaat gccctcatgc
    48901 gccagataca ctcgcccaaa aacattcagc gcggtaaaca gctgatataa agcgtcacgg
    48961 gtcgccttag gatcggcgat gtggaaatac ttataaaacg aaatggtggt tcgcggttcg
    49021 ctctcagcca acattttggc ttttaacgcg tcgttggaaa tgcggttgtg taacactggc
    49081 atggtgtacg attcctgcga gatgggaaag taaaaatccg cggcatgata tagcaattat
    49141 cgataattaa catccacata ttttacgcta catttgcgca ttaaaaatta tttgttattt
    49201 acaagcgcgg caatttcgcc cagtcttcag ccacaaattt tggttgcggg cgaaaaaatg
    49261 cgacaataca tacaattgcc cgaataggtt gaaaaacagg attgatatga cgaatctacc
    49321 caagttctcc accgcactgc ttcatccgcg ttattggtta acctggttgg gtattggcgt
    49381 actttggtta gtcgtgcaat tgccctaccc ggttatctac cgcctcggtt gtggattagg
    49441 aaaactggcg ttacgtttta tgaaacgacg cgcaaaaatt gtgcatcgca acctggaact
    49501 gtgcttcccg gaaatgagcg aacaagaacg ccgtaaaatg gcggtgaaga atttcgaatc
    49561 cgttggcatg ggactgatgg aaactggcat ggcgtggttc tggccggacc gccgaatcgc
    49621 ccgctggacg gaagtgatcg gcatggaaca cattcgtgac gtgcaggcgc aaaaacgcgg
    49681 catcctgtta gttggcatcc attttcttac actggagctg ggtgcgcggc agtttggtat
    49741 gcaggaaccg gggattggcg tttaccgccc gaacgataat ccactgattg actggctaca
    49801 aacctggggc cgtttgcgct caaataaatc gatgctcgac cgcaaagatt taaaaggcat
    49861 gattaaagcc ctgaaaaaag gcgaagtggt ctggtacgca ccggatcatg attacggccc
    49921 gcgctcaagc gttttcgtcc cgttgtttgc cgttgagcag gctgcgacca cgaccggaac
    49981 ctggatgctg gcacggatgt ccggcgcatg tctggtgccc ttcgttccac gccgtaagcc
    50041 agatggcaaa gggtatcaat tgattatgct gccgccagag tgttctccgc cgctggatga
    50101 tgccgaaact accgccgcgt ggatgaacaa agtggtcgaa aaatgcatca tgatggcacc
    50161 agagcagtat atgtggttac accgtcgctt taaaacacgc ccggaaggcg ttccttcacg
    50221 ctattaaatc tcccatgccg gatgcttcag aatggcatcc ggcattacca cagcaaatcc
    50281 ccctgattta gcgataaaag ctctctggat tgcgccccct ggaagtcggg cgcataatta
    50341 gtgtgcttat cttttcttct tatgttcacc gcgcctggcg caccaacagc ggattgctat
    50401 gtcaccctgt gaaaatgaca cccctataaa ctggaaacga aacctgatcg tcgcctggct
    50461 aggctgtttt cttaccgggg ccgccttcag tctggtaatg cccttcttac ccctctacgt
    50521 tgagcagctt ggcgttaccg gccactccgc cttgaatatg tggtccggta ttgtcttcag
    50581 cattacattt ttattttcgg ccatcgcctc accgttttgg ggtggactcg ccgaccgtaa
    50641 aggccgaaaa ctcatgctat tacgttctgc tctcggcatg ggcatcgtga tggtgttgat
    50701 gggactggca caaaatatct ggcagttttt gatcctgcgg gcgcttcttg gtttacttgg
    50761 cggatttgtc cccaacgcta atgctcttat cgccacacaa gtaccgcgta ataaaagcgg
    50821 ctgggcgctg ggtacgctct ccacaggcgg cgttagtggt gcgttgctcg gcccaatggc
    50881 tggcggcctg ctcgccgata gctacggctt acgtccggta ttctttatta ccgccagtgt
    50941 gctcatactc tgcttttccg tcaccctgtt ctgcatcaga gaaaaattcc agccggtcag
    51001 caaaaaagag atgctgcaca tgcgggaagt ggtgacatca cttaaaaacc cgaaactggt
    51061 actcagcctg tttgtcacca cgttaatcat ccaggtagcg acgggctcaa ttgcccccat
    51121 tctgacgctg tatgtccgcg aactggcggg taacgtcagt aacgtcgcct ttatcagtgg
    51181 catgatcgcc tcggtgccag gcgtggcggc tctgctgagt gcaccacgac tcggcaaact
    51241 tggcgatcga atcggccccg aaaagatcct gattacggcg ctgatctttt ctgtactgct
    51301 gttgatccca atgtcatacg ttcagacgcc attacaactt gggattttac gttttttgct
    51361 cggtgccgcc gatggtgcac tactccccgc cgtacagacc ctgttggttt acaactcgag
    51421 caaccaaatc gccggacgta tcttcagcta taaccaatcg tttcgtgata ttggcaacgt
    51481 taccggacca ttgatgggag cagcgatttc agcgaactac ggtttcagag cggtatttct
    51541 cgtcaccgct ggcgtagtgt tattcaacgc agtctattca tggaacagtc tacgtcgtcg
    51601 tcgaataccc caggtatcga actgattttt cgcctttcat acttgcaaaa gcggagaatc
    51661 agctatcctt ttccctgaaa cctcatcaac tcaaagggag aatcgtgatg accatgtacg
    51721 caacgcttga agaagccatt gacgctgcac gcgaagaatt tcttgcagac aaccccggca
    51781 ttgacgccga agacgcgaat gtgcaacagt tcaatgccca aaaatacgtt ttgcaggacg
    51841 gcgacatcat gtggcaagtt gagttttttg ccgacgaagg ggaagaaggt gaatgtttac
    51901 ctatgcttag cggtgaagcc gcgcaaagtg tttttgatgg cgactatgat gagatagaga
    51961 tccgccagga gtggcaggaa gagaatacat tacatgaatg ggacgagggg gaatttcagc
    52021 ttgagccacc gctggatacc gaggaaggac gcgcagcagc tgatgagtgg gatgaacgtt
    52081 aatcactcat acgggccatg atgaatgtcg atcggcagca gtaaagtatc aaaaaccagg
    52141 gagaatggca gatcaaggat tgtgacataa cgccaggcgg agtcacgcac atcccattgc
    52201 acaccgggat aatattggtt gccatgcccc tgccccggta tagtgcggct aatgatgctg
    52261 ccacagccac tcagcaaggt caccatgatg ctcaccacaa ttaaacgcat ttgatacctc
    52321 ttccgtaaaa aaataccggc ataacgccgg tatttttatt ggctcgttgt ttttgccaga
    52381 tgcgacgtga acgccttacc caacctacaa aatcttgcaa aatcaataaa ttgcaggaac
    52441 gatgtaggcc tgataagcgt agcgcatcag gcaactacgt ttttattgcg aagccgcatc
    52501 cggtttacgc aatgccagtg gattgagctt tatcccttcg taataactca cccatgaaga
    52561 atatctctca ggggaattcc agacgcggaa atgcagacgg gccatcgtca ccggatcgct
    52621 tagcagcacc aggcgacgat cgcgattcag cttctctggc gtctcgttca gcgcctgttc
    52681 aacgtggcgg tcacgggcga tttccagcac cttactggcg cggtgacgcg cggtcgccat
    52741 tgcggttgcc agagcgttaa atgacgggtt aaacactgcg tgcataaaac catcatcaag
    52801 ggagcgttga cggttcatct caaggaaccg atcggtatca accagtacct gcggcggtga
    52861 atactcttcc gggatcagga acagtttcca gcgtttggtg cgcagaccaa cggtggcacg
    52921 gctggaaatc accgaaacaa acggtgacag gatcaaagag aagacaatcg gtgccagcca
    52981 gaacaggaaa cgcagatcca gccatgccat cccaacagcc cacactaacc ctaacagcag
    53041 ctgtgagccg tggcgtttga acgcttcacc ccaggaagtg gagtcatcat cacgctgcgg
    53101 tgaattccac accacttccc agccaaggaa cgcgctgacg acgaagaccg tatggaacag
    53161 catgcgtacc ggagccagca gcacggaaaa cagcacttcc agcagcaacg ataatgtaac
    53221 gcgccagaag ccgccgtatt ctttcgttcc tttgcaccag ataagcaaaa tgctcaataa
    53281 cttcggcagg aacaacagca ccatggtcga agcaaaaagt gcaatcgcca gctcaggacg
    53341 ccactgcggc cacaccggga acaactgccg tggttgcagg aagtattgcg gttcggtcaa
    53401 cgcatgtact acctgcaatg cagtagagag cgcgaggaac ataaaccaca gcggagcgga
    53461 gagataagac atcacgcccg tcaggaacac cgcacggtga accgggtgca tacccttcac
    53521 caggaacaga cggaagttca tcaggttacc gtggcaccag cggcggtcac gttttagctc
    53581 atcaagcaag ttaggcggca attcttcata agaacccggg agatcgtaag caatccagac
    53641 cccccaacct gcacggcgca tcaacgccgc ttccacgaag tcatgtgaca ggattgaacc
    53701 ggcaaaggaa ccttcgcccg gcagcggagc cagtgcgcag tgctcgataa acggtttcac
    53761 gcggataatc gcgttatgcc cccagtagtg cgactcgcca agttgccaga agtgcaaacc
    53821 ggcagtgaac agtggcccat acacgcgggt cgcgaactgc tgacagcgcg catacagcgt
    53881 atccataccg gacgctttcg gcgacgactg aatgatcccg gcgttcgggt tggcttccat
    53941 caggcgcacc agcccgcaca aacaatcacc ggtcattacc gagtcagcat ccagcaccac
    54001 catgtagctg tactggctgc cccagcgacg gcagaagtca tcgatattac cgcttttacg
    54061 cttcacgcgg cgacggcggc ggcgatagaa aatctgccct tcgccaccga cttcagcgat
    54121 aagctccatc caggcttttt gctctgcgac gcagatatcc gggttataac tgtcactcag
    54181 aatgtagaca tcaaagtgtt tggcattccc ggtggctttt actgattccc acgttgcacg
    54241 caagccagca aaaacacggt tcacgtcttc gttacagata ggcatgatca acgccgtgcg
    54301 atgctccggg tttaatggtt catcgccaac tgttgacgca gatatactgt atttatcgcg
    54361 accaataagc agttgcagga agcccattaa cgccgtccag aatccggcgg acacccaaca
    54421 gaacagtacc gcaaagagga tcaggatacc ggtttgcagc atataaggca gaagctgcat
    54481 aaaggaaacc cacaaatcct gaccaaccat atccatagga ttaatcagcg cccaaccctg
    54541 ataaggaaga atggtcttca tataccaggt cgcgacgaca gtttgcgcga gcgtcaggat
    54601 caacagaatg taacggcgga tggtaccgac ggtacgccac ttttgctcgc tctcctgctc
    54661 ttctttggtc aaacgagcca gatagcgcgg cgtgacatcg cgtccacgca ggcgatccca
    54721 gaaacggcct accgggttgg tgcgccacgg gtcgggaaac atcgaggagc gttttgcttc
    54781 tggcatcgcc ttaagctgat cgcgcccttc gtcgtcttta attaactgtc catcagcaag
    54841 tgaatctggc caggcttgtt ccagacgcgc ctttaccgag ccttgcgggg aatcatcctc
    54901 ccgctcccag gtgcggtgtt cggcatccag cgcctgatga acggcgcgga tatcagtctt
    54961 cggcaatgcc gctttctcgc ttgcggcgat gggcattgcg tcaatgtact cagttgtctt
    55021 attcattggc aggtaactgg tagctccagg tttcactcaa cgtctgatcg gcattcacca
    55081 gcgcagcgcg catttcagtg gttttcttgg catctttcac tttcacacgc atcaccagac
    55141 gccagccttt ggtgaccggg ttatagcgca ccgtgctttc cactatctca ccattatcac
    55201 caatgctggt ttgcgctgtg accggggtat cctctggcag ttttttcatc tctgcgccgg
    55261 taaaatcgac cacaaaggcg atagtaccgt caggctggcg gatcaggttc gactgcttca
    55321 catcccccgt tgaacgacgc gtttgttgca cccatgcgtt atctggcgca tgcagtttgt
    55381 cttcatcacg gctgaaggtg atggtgtatt taaagttcat ctctttaccc ggctccggca
    55441 gctgatccgg cgtccagtaa gcgacgatgt tatcgttggt ttcatcgttg gttggaattt
    55501 ccaccagctc aacgctgcct ttaccccact cccctttcgg agtcacccat gcgcttggac
    55561 ggagatcgta acgatcatcg agatcttcaa agcgggagaa atcacgaccg cgctgcaaca
    55621 gaccaaagcc ttgcgggttt tccatggaga agctgctgac cgctaaatgt ttcgggttat
    55681 tcaacggacg ccagatccat tcgccattac cggcatggat agagagaccg ttagagtcgt
    55741 gcaactccgg acgatagtta tttgcaggcg acggttggtt cggcccaaac aggaacatac
    55801 tggttaacgg tgcaaccccc agtttgccga ctttatcgcg cagatagatt ttcgactgca
    55861 catccacaac cgtgtcacgc cctggcataa ctacgaattt gtaagcacct gtcgcgcgcg
    55921 gcgagtcaag caatgcataa atggttaaac gtttatcagt tggttttgga cgctcgatcc
    55981 agaactcttt gaagcgtgga aattcttcac ccgatggcaa ggcggtatca attgccagac
    56041 cgcgggcaga aaggccataa acctgacctg caccaatcac gcggaaatag ctggccccga
    56101 gcatgctgac gatttcatcg tttttatctt tgctgttgat cgggtaaagc actttaaaac
    56161 cggcaaaacc aaggtctttt accgtgtctt tgtcatgctg aacatcgccg aaagtgaaat
    56221 aatccgggct gtatttgatt cgtttgacgg cggtggcagt cacttcattt attttgaccg
    56281 gggtatcgaa gtacataccc tgatggtaga actcgagttt gaatggggtc ttcagattgt
    56341 tccagtacgc tttgtcatga ttaaactgga tctgctgata gtccgcgtat ttcatatcgc
    56401 ggaaaacgga gggcaagttg cttttgggcg cctcatagcc tttcccggct aaggattgag
    56461 cttgctttgc gacatcatca atactgaaag cccagcttga agatgtatac agggttaaca
    56521 ttactgcagc actcaaccaa cgcattttca tcatttgtag tttatgtttc ataattagta
    56581 agcacttccc cctttgtgtg cttatatcga tccgatctat tttaatggat aaccagccat
    56641 tccgacaaca gaatcccggt atcgttcagc acgctggctc atggattaag cacctgattg
    56701 caaccgtttt cactttccgt gcttctccta aagacagaag catcgacttc gtgtagggtt
    56761 tgccagcaaa tttatttatc aaccgtctta cagacgcttc cattctggtt agtggtagcg
    56821 gtgatgctat gcataaaaac ttgaagcctg cgcctggcgc ggtcaggtcg gtgtcgctat
    56881 ggcaacaagc ctaaaggcat aacccgacag ggataagacg aaaaatccga gattagttaa
    56941 ccatatatga atccagtacc cgcgcaacgt gaatatttcc tcgactccat ccgcgcctgg
    57001 ctgatgttgt tagggatccc ttttcatatt tctttaatct attcgagcca tacatggcat
    57061 gtgaatagcg ccgaaccgtc attgtggctg acccttttta atgacttcat ccactcgttc
    57121 cgcatgcagg tatttttcgt tatatccggc tacttttcct acatgctttt tttacgctat
    57181 cccttgaaaa aatggtggaa agtacgtgtc gaacgtgtag gtatcccgat gttaacagcc
    57241 atccccctac tgacattgcc gcaatttatt atgctgcaat acgtcaaagg gaaagcggaa
    57301 agttggcctg gactgtcatt gtatgacaaa tataatacgt tggcctggga attaatatcg
    57361 cacctgtggt ttttattggt gttagtggtc atgacgacgc tgtgcgtctg gatattcaaa
    57421 cgcatcagaa ataatttaga aaattctgat aaaacgaata aaaaattctc gatggtaaaa
    57481 ctatcggtga tttttttgtg cctcggcatc ggctatgcgg taataagaag aacgattttt
    57541 attgtgtatc cgcccattct gagtaacggc atgttcaatt ttattgtcat gcaaacgcta
    57601 ttttatttgc cgttctttat cctcggcgca ctggctttca ttttccctca tcttaaagcc
    57661 ttgtttacca cgccgtctcg tggctgtacc ctcgcagcag cattggcatt tgtcgcttac
    57721 ttactcaacc agcgctatgg cagtggcgat gcctggatgt acgaaaccga gtctgtgatc
    57781 accatggtcc tcggtctgtg gatggtgaat gtggtcttct ccttcggcca ccgtttgctt
    57841 aacttccagt cagcgcgggt gacttacttt gttaacgcat cgctatttat ctatctggtt
    57901 caccacccgt taacgctgtt tttcggcgcg tacattacac cgcacatcac ctccaactgg
    57961 cttggttttc tctgtggcct gatattcgta gtagggattg cgataattct gtatgaaatt
    58021 catttacgca tcccattact gaagtttttg ttctctggta aaccggttgt taagcgtgag
    58081 aacgataaag caccagcccg ttaagccgcg tttacaataa ccattccacg ggcaatatcg
    58141 acgccagtct gaccataacc cgcttccaga aactggtggc gggttctttt ttgagaacaa
    58201 tctctttctt tgcatgacga tcaacccagt tgatccgtcc ccacctgtcc agacggagct
    58261 gccaggccgc atcatactgg ctctgaataa agcgtttatc aattaactgt gccagcgttt
    58321 cgctctctat cacgaagccc atttcagtat tgagcaatgt tgaacgcgga tcgaaattga
    58381 aagagccgat aaacaccgtt ttaccatcga tgctaaaggt tttagcatgc aggctggcac
    58441 cggaattacc tgttatgccg cgatcgtgta acgtactact ttgttcacac gtcggcttga
    58501 gttcatataa ttccacgcca taacggagca attttttgcg ccagcgcgcg tatccggcat
    58561 ggacgacagc aacatcgtta gcggcaagag aattggttag gatcgcaatc tttacccctt
    58621 ttctcaccat ccgtaagagt tgcgccacac ctgcgcgtgt cggtacaaaa taggaagaga
    58681 taatatcgat gcgttcactg ggtgagccca tgatatcgaa caggcgctgc ggtaacagtg
    58741 aatgccgttt tgccttgccc tcccctttcg ccggatcatc acttaataaa cgtgtcttcg
    58801 cccagataag cggcaacgtt ccatcaacca gatgatttat aaacggactg gattccattt
    58861 tgcgtaaata acgatgcgtc atggcatcgt tatgccagga ggcgggtaac tcgatgcgat
    58921 ccgccatttc accttccggg acatccagca cctgctgtaa gggtgaaacc gatttgcaat
    58981 accagtagcg ggcgaaatca tcggcaacgt cctctactac gggtcctatt gccatgacat
    59041 ctaaatccga aaaaagtggc tcttcccctg ccccaaaata ggcatcacca atatttcgcc
    59101 cccctaccag cgtcaccacg ccatcgacag tgaaactttt attgtgcata cggcgattga
    59161 ggcgggaaaa gtcggtgata taaccaagcg gacgcagcaa gcgaaacgag aaaggattaa
    59221 aaagccggac ttcaatgcgg ggatggctgt caagcaagcg taaaatgtcg tcaagtccgg
    59281 gcgtattgtt gtcgtccagc aacaaacgga cgcgaacgcc acgctttgct gcggctaaca
    59341 gggcggaaaa cagtaaccga cccgacatat cgtcctgcca gatgtaatac tgaacatcca
    59401 gcgtatgctc tgccatttcg gccagacgat accgggcggc aaacgcatcc aaacttttct
    59461 ccagagggaa aagaccacac tgaccgggat gttgcgaaca cagtggcagc accgcgctcg
    59521 ccagccgggg caaatcattc atctccttgt tgggtaagga gtctttcgta gaggtgggcg
    59581 ttttcttcat cataacagac aaagtatacc tgttcgggta aagcgtgacg ggtaataaat
    59641 tctgaaacgg ttttaactgc gatttcagcc gctgccgcac gagggtaacc ataaacccca
    59701 gtactgattg caggaaaagc cactgacgta tagctgtttg ccgccaccag tcgcaggcta
    59761 ttcagatagg catcctgcaa aagctggtct tcgttttgtt caccaccacg ccagactggc
    59821 ccgacggtgt gtactacggc tttagcggga agatcgcctg caagcgtaat aacggcatgc
    59881 cccgtagggc aatcgccctg ctgttgcctg acttttaaac aggcatccag cagggccgga
    59941 cccgctgcgc gatgaatggc cccatcgacg ccgccgcctc ccattaatga cggattagcc
    60001 gcattcacaa tcacatcaac ggccagtttg gtaatatcac cctgcacaac atgaatacgc
    60061 gttttcataa cgcctcctta caccttcttg tttcttaagt gtacagcagg ctttttggtt
    60121 aaatcgcatt tttctgctca gcaacgtgcg ttgttcagaa acttagttat attcgatgtt
    60181 cattacagcc agctttttct gtttatcaag atactgcact tttactgagg ctatttgcgc
    60241 gtttttgaca ttgccgctca ttaacgcttt gctggaatag gttttgcccg gaacagaacc
    60301 attctgtggg cagttcattt ctacgggtga tgactgggtg ctgacgtcac atgccggttc
    60361 cacaatttgg ccataaaaat ggatcacacc cccttgcgcg gtgcttcctg cgatggcaat
    60421 aaaaggaaaa aacaaactgc cgagcataag agagtttaaa aaaggtctga acatttattc
    60481 tatttcctca atgattagtc atccttgagg gttgtgttat ccatactttc aggttggctg
    60541 attcgcagca gaccattctc tccagattca tcttatgctc gatatttcaa caaattaaga
    60601 cttttctgaa gagggcggcc attgttgtga taaatgaagt gcctgtccat cagaaacagt
    60661 aacaactatt ttcacccgat cgtccgggga aatatttaaa ctcaacttcg tcaaagcaat
    60721 aggttgatta gcaggcaatg agagggtctt ttcttgcttc gtctgacttt gccctgaact
    60781 gccttcgcgc agggacaata tttgtactct gcacagacaa gattgagtaa gagtgacttc
    60841 aggaataatg gtatacacat ccccttgctg ggtcgtatta aaggttatct ggctggaaag
    60901 tgccgcaagg agtaataacg cattcatatt cttctcccga aaaaaaacag ggcttgcgcc
    60961 ctgtttctgt aatacaaatg atgtattagt actgatgagc ggtcgcgttg ttaccaaagc
    61021 caacctgagt cacgttgacg gaggagttag atgcagtctg gtcaactgca gcaccgttgc
    61081 caccaccgaa ctgtttaaca gtcatttcag aatttttgcc gttccactga tcaagagtgg
    61141 cgctgttacc gaagccacgt tgggtcagat cgattgagct gtcatctgag ccctgaccaa
    61201 catctgcacc attaccaccg ccatgctggg taatagtcaa gtcagagtta cgggcatcag
    61261 tttgcagagc aagtgcagag ttaccgccac cgtactggta aatgttcagc tctgaatttg
    61321 ggccgctatt attaccgcca ccaccgtggt taccgccgcc gccgtactga ggaacaacac
    61381 ctgccagagc gctaccggag aatacgattg ctgcaattgc tgctactttt aaaagtttca
    61441 tgtaaaaccc ccatcggatt gatttaaaag tcgaatggaa attaacgttg tgtcacgcga
    61501 atagccattt gcgactgtct ctgcactaca attgccgttt tttgagtacc atactgtgta
    61561 atatttgctt tattaccaga acctttctgg ataatcatcg cagtattacc ataagcacct
    61621 tgcgaaatac tggcatcgtt ggcactgccc gcctgatcaa tatatgcaag gttataatct
    61681 cctgtctggt caatctttgc ccggttgcta ctaccttctt gcgcaacaac cgccaaaagt
    61741 tttgagcctc cctgccgtaa ctgagcacta ttattagtcc cagcttgacc aattatggct
    61801 gcctgattaa atgaagactt actcaattca tttaccgcga agttatattc tgaattagct
    61861 aaatcataac ctgctgcggc tgcaatccca ggcgcaccca gtattgttaa catcataaat
    61921 aacaatttgt tttt
//
LOCUS       DAPGOF010000004        60678 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.004, whole genome shotgun sequence.
ACCESSION   DAPGOF010000004 DAPGOF010000000
VERSION     DAPGOF010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 60678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.004"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(116..1372)
                     /gene="xapB"
                     /locus_tag="RHV13_000189"
     CDS             complement(116..1372)
                     /gene="xapB"
                     /locus_tag="RHV13_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HDV1239756.1"
                     /translation="MSIAMRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKTVMLMSMVAWTLRFGFFAYGDPSTTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWQTIWLVFAGYAL
                     FLAVIFFFGFKYNHDPEKIKHRAVAH"
     gene            complement(1432..2265)
                     /gene="xapA"
                     /locus_tag="RHV13_000190"
     CDS             complement(1432..2265)
                     /gene="xapA"
                     /locus_tag="RHV13_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HDV1239757.1"
                     /translation="MSQVQFSHNMLFCIDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGYLQGVPVVCMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDDRFGERFFSL
                     ANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCDLKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            2514..3278
                     /locus_tag="RHV13_000191"
     CDS             2514..3278
                     /locus_tag="RHV13_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1239758.1"
                     /translation="MKKHLLTLTLSSILAIPVVSHAEFKGGFADIGVHYLDWTSRTTE
                     KSSTKSHKDDFGYLEFEGGANFSWGEMYGFFDWENFYNGRHNKPGSEQRYTFKNTNRI
                     YLGDTGFNFYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYNFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            complement(3317..4243)
                     /locus_tag="RHV13_000192"
     CDS             complement(3317..4243)
                     /locus_tag="RHV13_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV1239759.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            4333..5331
                     /locus_tag="RHV13_000193"
     CDS             4333..5331
                     /locus_tag="RHV13_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HDV1239760.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(5328..5546)
                     /locus_tag="RHV13_000194"
     CDS             complement(5328..5546)
                     /locus_tag="RHV13_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HDV1239761.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(5548..7563)
                     /gene="ligA"
                     /locus_tag="RHV13_000195"
     CDS             complement(5548..7563)
                     /gene="ligA"
                     /locus_tag="RHV13_000195"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDV1239762.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIDVIDEAEMLRLLGS"
     gene            complement(7634..8632)
                     /gene="zipA"
                     /locus_tag="RHV13_000196"
     CDS             complement(7634..8632)
                     /gene="zipA"
                     /locus_tag="RHV13_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HDV1239763.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSA
                     PQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLN
                     SIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQV
                     PSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            8862..9623
                     /gene="cysZ"
                     /locus_tag="RHV13_000197"
     CDS             8862..9623
                     /gene="cysZ"
                     /locus_tag="RHV13_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HDV1239764.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            9808..10779
                     /gene="cysK"
                     /locus_tag="RHV13_000198"
     CDS             9808..10779
                     /gene="cysK"
                     /locus_tag="RHV13_000198"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDV1239765.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            11163..11420
                     /gene="ptsH"
                     /locus_tag="RHV13_000199"
     CDS             11163..11420
                     /gene="ptsH"
                     /locus_tag="RHV13_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HDV1239766.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            11465..13192
                     /gene="ptsI"
                     /locus_tag="RHV13_000200"
     CDS             11465..13192
                     /gene="ptsI"
                     /locus_tag="RHV13_000200"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HDV1239767.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            13233..13742
                     /gene="crr"
                     /locus_tag="RHV13_000201"
     CDS             13233..13742
                     /gene="crr"
                     /locus_tag="RHV13_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDV1239768.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(13784..14635)
                     /gene="pdxK"
                     /locus_tag="RHV13_000202"
                     /gene_synonym="thiJ"
     CDS             complement(13784..14635)
                     /gene="pdxK"
                     /locus_tag="RHV13_000202"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HDV1239769.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            14740..15108
                     /locus_tag="RHV13_000203"
     CDS             14740..15108
                     /locus_tag="RHV13_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HDV1239770.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(15111..16022)
                     /gene="cysM"
                     /locus_tag="RHV13_000204"
     CDS             complement(15111..16022)
                     /gene="cysM"
                     /locus_tag="RHV13_000204"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HDV1239771.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPNNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(16157..17254)
                     /gene="cysA"
                     /locus_tag="RHV13_000205"
     CDS             complement(16157..17254)
                     /gene="cysA"
                     /locus_tag="RHV13_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HDV1239772.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPEQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(17244..18119)
                     /gene="cysW"
                     /locus_tag="RHV13_000206"
     CDS             complement(17244..18119)
                     /gene="cysW"
                     /locus_tag="RHV13_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HDV1239773.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(18119..18952)
                     /gene="cysT"
                     /locus_tag="RHV13_000207"
     CDS             complement(18119..18952)
                     /gene="cysT"
                     /locus_tag="RHV13_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HDV1239774.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(18952..19968)
                     /gene="cysP"
                     /locus_tag="RHV13_000208"
     CDS             complement(18952..19968)
                     /gene="cysP"
                     /locus_tag="RHV13_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HDV1239775.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKGKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKNKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(20139..20930)
                     /gene="ucpA"
                     /locus_tag="RHV13_000209"
     CDS             complement(20139..20930)
                     /gene="ucpA"
                     /locus_tag="RHV13_000209"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HDV1239776.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(21059..21916)
                     /gene="murR"
                     /locus_tag="RHV13_000210"
     CDS             complement(21059..21916)
                     /gene="murR"
                     /locus_tag="RHV13_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HDV1239777.1"
                     /translation="MLYLTKISNAGSEFTENEQKIADFLQANVSELQSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            22080..22976
                     /gene="murQ"
                     /locus_tag="RHV13_000211"
     CDS             22080..22976
                     /gene="murQ"
                     /locus_tag="RHV13_000211"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDV1239778.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            22980..24404
                     /gene="murP"
                     /locus_tag="RHV13_000212"
     CDS             22980..24404
                     /gene="murP"
                     /locus_tag="RHV13_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HDV1239779.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFCCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            24409..25713
                     /gene="pbp4b"
                     /locus_tag="RHV13_000213"
     CDS             24409..25713
                     /gene="pbp4b"
                     /locus_tag="RHV13_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HDV1239780.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(25771..26670)
                     /gene="yfeX"
                     /locus_tag="RHV13_000214"
     CDS             complement(25771..26670)
                     /gene="yfeX"
                     /locus_tag="RHV13_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HDV1239781.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(26766..27341)
                     /locus_tag="RHV13_000215"
     CDS             complement(26766..27341)
                     /locus_tag="RHV13_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HDV1239782.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(27548..27997)
                     /locus_tag="RHV13_000216"
     CDS             complement(27548..27997)
                     /locus_tag="RHV13_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HDV1239783.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWRVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGFGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(27984..28409)
                     /locus_tag="RHV13_000217"
     CDS             complement(27984..28409)
                     /locus_tag="RHV13_000217"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HDV1239784.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            28623..29492
                     /gene="amiA"
                     /locus_tag="RHV13_000218"
     CDS             28623..29492
                     /gene="amiA"
                     /locus_tag="RHV13_000218"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HDV1239785.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            29496..30395
                     /gene="hemF"
                     /locus_tag="RHV13_000219"
     CDS             29496..30395
                     /gene="hemF"
                     /locus_tag="RHV13_000219"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HDV1239786.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(30401..31453)
                     /gene="eutR"
                     /locus_tag="RHV13_000220"
     CDS             complement(30401..31453)
                     /gene="eutR"
                     /locus_tag="RHV13_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HDV1239787.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(31499..31999)
                     /gene="eutK"
                     /locus_tag="RHV13_000221"
     CDS             complement(31499..31999)
                     /gene="eutK"
                     /locus_tag="RHV13_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HDV1239788.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESAGELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(32012..32671)
                     /gene="eutL"
                     /locus_tag="RHV13_000222"
     CDS             complement(32012..32671)
                     /gene="eutL"
                     /locus_tag="RHV13_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HDV1239789.1"
                     /translation="MPALDLIRPSVTAMRVIASVNADFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAQDTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(32681..33568)
                     /gene="eutC"
                     /locus_tag="RHV13_000223"
     CDS             complement(32681..33568)
                     /gene="eutC"
                     /locus_tag="RHV13_000223"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HDV1239790.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTTCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRLRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(33589..34950)
                     /gene="eutB"
                     /locus_tag="RHV13_000224"
     CDS             complement(33589..34950)
                     /gene="eutB"
                     /locus_tag="RHV13_000224"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HDV1239791.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(34962..36365)
                     /gene="eutA"
                     /locus_tag="RHV13_000225"
     CDS             complement(34962..36365)
                     /gene="eutA"
                     /locus_tag="RHV13_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HDV1239792.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKSLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAALIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(36362..37588)
                     /gene="eutH"
                     /locus_tag="RHV13_000226"
     CDS             complement(36362..37588)
                     /gene="eutH"
                     /locus_tag="RHV13_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HDV1239793.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKDDATAAKTEAEAQS"
     gene            complement(37905..39092)
                     /gene="eutG"
                     /locus_tag="RHV13_000227"
     CDS             complement(37905..39092)
                     /gene="eutG"
                     /locus_tag="RHV13_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HDV1239794.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMSGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAAQEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(39082..39918)
                     /gene="eutJ"
                     /locus_tag="RHV13_000228"
     CDS             complement(39082..39918)
                     /gene="eutJ"
                     /locus_tag="RHV13_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HDV1239795.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDREDQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPVLQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(39929..41332)
                     /locus_tag="RHV13_000229"
     CDS             complement(39929..41332)
                     /locus_tag="RHV13_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HDV1239796.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     IAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAGKIA
                     AAIGLKVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(41344..41631)
                     /gene="eutN"
                     /locus_tag="RHV13_000230"
     CDS             complement(41344..41631)
                     /gene="eutN"
                     /locus_tag="RHV13_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HDV1239797.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(41738..42031)
                     /gene="eutM"
                     /locus_tag="RHV13_000231"
     CDS             complement(41738..42031)
                     /gene="eutM"
                     /locus_tag="RHV13_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HDV1239798.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(42070..43086)
                     /gene="pta"
                     /locus_tag="RHV13_000232"
     CDS             complement(42070..43086)
                     /gene="pta"
                     /locus_tag="RHV13_000232"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDV1239799.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGGKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(43083..43886)
                     /gene="eutT"
                     /locus_tag="RHV13_000233"
     CDS             complement(43083..43886)
                     /gene="eutT"
                     /locus_tag="RHV13_000233"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HDV1239800.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGD
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKPPLTVKQIQQRLGETQ"
     gene            complement(43883..44584)
                     /gene="eutQ"
                     /locus_tag="RHV13_000234"
     CDS             complement(43883..44584)
                     /gene="eutQ"
                     /locus_tag="RHV13_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HDV1239801.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(44559..45038)
                     /gene="eutP"
                     /locus_tag="RHV13_000235"
     CDS             complement(44559..45038)
                     /gene="eutP"
                     /locus_tag="RHV13_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HDV1239802.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(45051..45386)
                     /gene="eutS"
                     /locus_tag="RHV13_000236"
     CDS             complement(45051..45386)
                     /gene="eutS"
                     /locus_tag="RHV13_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HDV1239803.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(45679..47958)
                     /gene="maeB"
                     /locus_tag="RHV13_000237"
     CDS             complement(45679..47958)
                     /gene="maeB"
                     /locus_tag="RHV13_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HDV1239804.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALIGNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            48247..49197
                     /gene="tal"
                     /locus_tag="RHV13_000238"
     CDS             48247..49197
                     /gene="tal"
                     /locus_tag="RHV13_000238"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDV1239805.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            49217..51220
                     /gene="tkt"
                     /locus_tag="RHV13_000239"
     CDS             49217..51220
                     /gene="tkt"
                     /locus_tag="RHV13_000239"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDV1239806.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPTDKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(51320..52363)
                     /locus_tag="RHV13_000240"
     CDS             complement(51320..52363)
                     /locus_tag="RHV13_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HDV1239807.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(52489..53064)
                     /gene="nudK"
                     /locus_tag="RHV13_000241"
     CDS             complement(52489..53064)
                     /gene="nudK"
                     /locus_tag="RHV13_000241"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HDV1239808.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            complement(53132..55111)
                     /gene="aegA"
                     /locus_tag="RHV13_000242"
     CDS             complement(53132..55111)
                     /gene="aegA"
                     /locus_tag="RHV13_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HDV1239809.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHADDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
                     TARQEHQPWHASTAVQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHLTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGMAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            55317..57017
                     /gene="narQ"
                     /locus_tag="RHV13_000243"
     CDS             55317..57017
                     /gene="narQ"
                     /locus_tag="RHV13_000243"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HDV1239810.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYRSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGDNWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            57181..60294
                     /gene="acrD"
                     /locus_tag="RHV13_000244"
     CDS             57181..60294
                     /gene="acrD"
                     /locus_tag="RHV13_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HDV1239811.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(60393..60452)
                     /gene="ypfM"
                     /locus_tag="RHV13_000245"
     CDS             complement(60393..60452)
                     /gene="ypfM"
                     /locus_tag="RHV13_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HDV1239812.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT        14220 a        16739 c        15482 g        14237 t
ORIGIN      
        1 cacgattgta aacaaagcac caatgccaaa accaacgccg gaagaaaata aaatatcttt
       61 cactaattaa cctttatcat aaaagcagct ctgaagagca gagctgcgaa tccttttaat
      121 gagccaccgc tcgatgcttt atcttttcag ggtcatgatt atatttaaac ccaaagaaaa
      181 atatcactgc gagaaaaaga gcatatcctg caaacaccag ccagatagtt tgccagtctt
      241 ttacgccatc caccgaaaag taatctactg ccatgccact cagaatcgag ccaacccatg
      301 cgccgacacc atttaccatg gtcataaaga gcccctgcgc gctggcacga atgctggaat
      361 caacttcctg ttcgacaaat accgaaccag aaatattgaa gaaatcgaat gcacagccat
      421 aaacaatcat cgacagcagc agcaaaataa atccggttgt tgacggatcg ccataggcga
      481 agaagccaaa gcgcagcgtc caggccacca tactcatcag catgacggtt ttaatgccaa
      541 atcgctttaa aaagaatggg atagtcagta taaagcccac ttctgccatc tgtgaaactg
      601 acagtaaaat ggagggatat ttcaccacaa aactgtcagc aaactccggg ttacgggcga
      661 aatcatgtag gaacggatta ccaaaaacgt tggtaatttg cagtaccgca cccagcatca
      721 tggcaaagag gaaaaagatg gccatgcgtg gatttttaaa cagcacgaag gcatccagac
      781 ccagcttgct ggcaagcgat gtggtcgctt ttttctccgc aaccggaatc ttcggcaaag
      841 tcagcgcata agccgacagc agcaatgacg caccggacgc gatatacagc tgcagactac
      901 tcaattccag atgcagcagg cttactgccc acatcgcgac aatgaacccc accgtaccaa
      961 aaacgcgaat gggcggaaaa gcggtcaccg ggtcaagccc tgcctgggca agacaggaat
     1021 aagagacgct gttcgataac gcaatagtcg gcataaacgc catcgcattg actaacatca
     1081 cccaaaacat catatccgga tcagttacgg atgccgcata aaaaagtacg cccgcacaca
     1141 ccaggtgaca tagcatgtat gcacgttctg cacgcagcca tttgtctgcg atgatcccca
     1201 ttataccagg cataataatc gcggcgatcc ctttggaact gtaaaccatg ccaacattag
     1261 cgccggtgaa atgaagggta ttaatcatgt aagagccgag ggtaaccagc cagctccccc
     1321 agataaaata ttgcaaaaag gacattacct ttaagcgcat cgcgatgctc ataatcgttt
     1381 ccttgtcgca tcatttggcc tcacccgcga ggccgtttct tattgttttt ctcaggcaat
     1441 tttgcgcaga aagccacaaa taaggttgat gaagttctgc ttagagagct ccgcagccgc
     1501 cagcgtttgc gcatgtgaca atttcacatc gcttaaacct tccgccatat tggtaatcgc
     1561 agagaccgca acgactttaa ggtcgcaatg gcgagctgaa ataacctcag gcaccacaga
     1621 cataccaaca acatccccac caataatttg catcatgcga atttccgccg cagtctcgaa
     1681 attcggcccc ggatacgaga cgaacacgcc ctccgtcaga gggaaaccct cttctttcgc
     1741 cactttttgt aacagtgcgc ggtattccgc atcgtaggca tttgccagtg agaagaaacg
     1801 ctctccaaaa cgatcatcgt taagtcccac catcggcgta cctggcatgg tgttgatatg
     1861 atctttcaat gcgaccaggc tgcctgcccc cacttccgga cgcagcgagc ctgccgcatt
     1921 ggtgcagaac agtagctcac agcccagcag cttaaaggta cgaattgcgt cagtcataat
     1981 ggtcattcca cgaccttcgt agaaatgtcc gcgacctttc atacatacca ccggtacgcc
     2041 ctggagatag cccagcacta actcccccgc atgaccatgt accgtactta ccgggaagcc
     2101 cggcagcttt tcgtaggaaa ttgcgacagc gttctcaatc tgatcggcca gcgcgccgag
     2161 cccggaacct aaaataaagg ccactcgtgg cgtgaaatca ggtttataag tcttgataat
     2221 atctatgcaa aacagcatgt tatgagaaaa ttgaacctga gacatatata tcctttttct
     2281 gtagggtgga atctaacgcc tttttatagc gaggttgtcc cggctcttac caatacactt
     2341 ttctcacgca ttcaatagcg attcgatatc gatggattta tatctttcct ctattaatcc
     2401 acggtaaaaa ctgtattggc gatattaatt cctttgcatt aacaatatgt cgtttttagc
     2461 aaaccacttt attaagcaat ctactaataa cgatcggcat agggaattat gctatgaaaa
     2521 aacatctttt aactctgaca ctttcctcta tattagcgat acctgttgta tctcatgcag
     2581 aatttaaagg cggttttgca gatatcggcg tgcattatct ggactggacc agccggacta
     2641 ccgaaaaatc gtcgaccaaa tcacacaaag atgattttgg ctatcttgaa tttgaaggtg
     2701 gcgctaactt tagttgggga gaaatgtacg ggttcttcga ctgggaaaac ttctataacg
     2761 gtcgccataa taaaccaggt agcgagcagc gttatacctt taaaaatacc aaccgtattt
     2821 acctgggtga caccggattt aatttctact tgcatgcgta tggcacctac ggttctgcga
     2881 atcgcgtgaa tttccacgac gatatgttcc tgtacggtat cggctacaat ttcaccggca
     2941 gcggttggtg gttcaaaccg ttctttgcta agcgttatac agatcaaacc tattacactg
     3001 gcgacaacgg ctatgtcgcc ggctgggttg caggttacaa ctttatgctg ggcagcgaga
     3061 aattcactct taccaactgg aacgagtacg agtttgaccg tgacgctacc tatgcagcgg
     3121 gtaatggcgg taaagaaggg ctgaatggcg cagttgcact gtggtggaat gcaacatcac
     3181 acattactac ggggattcag tatcgatatg cggatgacaa actgggcgaa gatttttacc
     3241 aggatgcgat aatctactcc atcaaattta acttctgatt gattactgcc ggggctcccg
     3301 gcagtatgaa ttcactctat atctgataca acggatcccc ttcccgacca gccattagcg
     3361 cattgccctg gtctcgtacc acgtcccata acgcttcagc ggcggtggaa agtgaacggt
     3421 tcttcctgcg caccagcatc aactggcgtt caacaaccgg cgtaatgcgc tttaccacca
     3481 gtgggctacc ttcaggcaat ggtagcgcca gtgcgggaag aatactgatg ccgatgcctg
     3541 cggctaccat cgggaacagc gtcgccggat gtccaatctc ctgcacaata ttcgcctgaa
     3601 taccattgcg cgccagcgct gcgtcaatca gcggtcggct gcctgacgcg taatcctgca
     3661 acaccaattt cgctccctgt aatgcctgcc agggtacata atcttccacg gccaaagcgc
     3721 tatcgcgatg gcaaagcaga aagaaaggct cggaaagaat cgcttcacat tgcagatcgc
     3781 ccactgggcc tggatcgatg acgatgccaa agtcgacatc tccctggcga atactttcca
     3841 tcacccactg ctgcggacga tcgtgcagga caaactgaat atctggatag cggcgatggc
     3901 tttcagcaat gcattgcgga ataagatgcg cggaaatggt ctggctggca gcgacccgaa
     3961 cttttccgct cagttgttgc cccatacgcc cggtatcgcg caacgtgctg ttaagttcat
     4021 ccagcagtcg ctcaagacgc aaagccaact gctgccctgc atctgtaagc accacttcac
     4081 gcgtggttct gtccagcagg cgaacaccag tatgattttc cagctccttc acactgtgac
     4141 ttactgccga ctggctcagg ccgatacgct ctcctgcacg actaaaactt ttctcctgcg
     4201 ctactgtgac gaaaaccttt aattgtttta aagaataatt catctgtttt cttcatgaat
     4261 ggatgcaata aatcaatttt atttctcaaa ctggaagaag cacaatagaa ccatcgatca
     4321 tctggagtct ttatgaaact ttttcgtatc ctcgatcctt tcaccttaac cctgatcacg
     4381 gtggtgttgc tggcctcttt ctttccggcc agaggcgatt tcgtcccctt ctttgaaaat
     4441 ctgaccaccg cagctattgc cctgctgttc tttatgcacg gcgcgaagtt gtcgcgtgag
     4501 gcgattattg ctggcggtgg tcactggcga ctgcatttgt gggtaatgtg cagcaccttc
     4561 gtgctgtttc cgattctggg tgtactgttt gcctggtgga aaccggtaaa tgtcgacccg
     4621 atgctctact ccggttttct ctacttgtgc attctcccgg ctaccgtgca gtctgcaatc
     4681 gccttcacgt caatggcggg cggtaacgtc gcggcggcgg tttgttctgc gtcagcatcc
     4741 agcctgctgg ggattttcct ttcaccattg ctggttggtc tggtgatgaa tgttcacggt
     4801 gcagggggca gccttgagca ggtcggtaaa attatgctgc aactgctgct gccgtttgtg
     4861 ttggggcatc tttcccggcc gtggattggt gactgggtgt cgcgcaataa aaaatggatt
     4921 gcgaaaactg accagacgtc cattctgtta gtggtttata cagcgttcag cgaagccgtc
     4981 gttaatggta tctggcataa agttggctgg ggatcattgc tgtttatcgt ggtggtcagc
     5041 tgcgttcttc tggctatcgt gattgtagtt aacgtcttta tggcacgccg actgggcttc
     5101 aataaagcag atgaaattac tatcgtcttt tgtggttcga aaaagagtct ggcaaatggc
     5161 atcccgatgg caaacattct gttccccaca tcggtgatcg gtatgatggt gctgcccctg
     5221 atgattttcc atcagatcca attgatggtc tgtgcggtgc tggcgcgtcg atacaaacgc
     5281 cagaccgaac agttacaggc gcagcaggaa agcagcgccg ataaagctta aagcggacgc
     5341 ttcaggggct ggaccagttg cgtcagcccc tcggttttaa tcagcagcgt gatttgcatt
     5401 agctcaccga gcttccctgc cgggaactca tctttgcggg caaaccacag cagatactcc
     5461 tccggcaggt cgattaagcg acgccctttg tatttgccaa acggcattat cgtattggct
     5521 atttcaatca gctgctcttt ttccatctca gctacccagc aaacgcagca tttccgcttc
     5581 gtcgatgacg tcaataccca gttcctgcgc cttcgccagt ttagatcctg cagcttcacc
     5641 cgctatcacc agatcggttt tcttcgacac gctgcccgcg actttcgcgc ccagttcgac
     5701 cagtcgagct ttagcgtcat cacgtgacat ctgacttaag ctgcccgtaa gcaccacggt
     5761 tttaccagca aacgggctgt caatctcttc cgcgttgata acgatcggtg caggccagtg
     5821 aacaccttcc gccaacagct cgctgatgac attgcggttg ctttcttcgg caaagaagtt
     5881 gtgaacgtgt gatgcaacga caatgccaac atcaggcacc ttttgcagtt cttcaatcga
     5941 agcggcttcc agcgcttcca gcgtgccgaa atatgccgcc agacctgctg cggtggcctc
     6001 gccgacttca cggatgccaa gtgcatagag gaagcgagca aaggtggttt ctttcgcttt
     6061 ttccagcgcg ttaaccacgt tttgtgccga ttttggcccc atacgctcca gtccggtaag
     6121 ttttcctgcg gtgagtttga acagatccgc cggagtgtgg acatattctt tttcaaccag
     6181 ctgatcgatg attttgtcgc ccattccgtc aacatccatc gcacggcggg aaacaaagtg
     6241 tttcagcgac tctttacgct gcgcaccgca aatcaggcca ccggtacagc gggcaaccgc
     6301 ttcaccttcc acacgctcaa cgtcagaacc acataccgga caatgcgtcg ggaatacaac
     6361 ctcacgggta tcttccgggc gttcagaaag cacgacgtta accacctgcg ggatcacgtc
     6421 gccagcgcgg cgaatcacca ctttatcgcc aatgcgtaaa ccaagacgtt cgatttcatc
     6481 cgcattgtgt aaggttgcgt tactcaccag cacgcctgca acatggacag gttccagacg
     6541 cgcaacaggc gtaatcgcgc cagtacgccc aacctgaaac tcgacgtcac gcacaaaggt
     6601 catctgttcc tgcgccggga atttaaacgc taccgcccag cgcggggctc gcgcgacaaa
     6661 gccaagctgc tcctgctgtt ccagtgagtt gaccttaatc accacgccgt cgatatcaaa
     6721 gcccagcgtc gggcggtctt cttccacttt gtgatagaac gccagcactt cttccgccga
     6781 ttcacaaagc gttacccgat cgctgaccgg caacccccac tttttaaatt gcagtaaacg
     6841 gccaagatga gtatccggca gctcgccacc ttccagaaca ccgacgccat agcagaaaaa
     6901 agtgagcggt cgcttcgctg taatacgcgg atcaagctga cgcagtgaac cagctgccgc
     6961 attacgtggg ttagcaaaaa ctttcccgcc cgtgcgtcgc gcatcttcgt taatcttttc
     7021 gaaccccgcc tgcggcagga acacttcacc acgcacttcc agacgcgccg ggatattctc
     7081 tccgtgcagc ttcagcggaa tggcgcgaat agtacgcaca ttagacgtga tatcttcccc
     7141 ggtggtgcca tcgccacggg tcgcggcact gactaaaacg ccattttcat acagaatact
     7201 gacagcaagg ccatccagct tcagctcaca gcaccaggtg actttctcgt tgcttttcag
     7261 acggtcctgc actcgtttgt tgaaggcaag aaagctttct tcatcaaaaa cgttatccag
     7321 cgacagcatt ggcacttcat ggcgaatctg gctgaaagcc gccagcggcg cagcacctac
     7381 acgttgggta ggcgaatcag gcgtaatcag ttctggatgt ttggtttcca gctcgcgcag
     7441 ttcgcgcatc agcctgtcgt attcagcgtc gggaatttcc ggcgcatcca tcacatgata
     7501 aagatattca tgatggcgaa gcgtcgttcg cagttctgtc agttgttgtt cgattgattc
     7561 catatcgcac catcaatgct aaaaaccccc gacaagcggg ggttcgaaga ggagttaatt
     7621 tgccttaagt gtatcaggcg ttggcgtctt tgacttcgcg gatgatgtcc tggtactcac
     7681 gcaatttctg cggagtcatc atacggcgct gatcgtcaag cacgacaccg cccacttcat
     7741 cggcaatatg ctgcgcagat tgcagcatca gcttgaagtt ctgcagctcg tcaccgtaag
     7801 acggtacctg cataaagata gtgacacccg gagtagtgaa atccttcatt tcaggatcaa
     7861 aggttcccgg tttcaccata ttcgccaggc tgaataacgc cgggccgctg ccatccgggc
     7921 taagatgacg atggtaaata ttcatatcgc caaaaatgaa gcccgcttgt tgaatgctgt
     7981 taagaagcag ttcaccgttt agctcgctac cgtgatgcgc cgcgacgttc atgataatca
     8041 ccgcttcttt gcgcttcggt ttatccataa ctggagccgg ttccgctaca ggctcaggct
     8101 gtggtgccgc tacgggttct gcaggctgga aagcctgttg tgccggttgc ggtgctgaat
     8161 gcactggctg cggcgctggc gcgacctgtg gcgaaactgg ctgctgcaac ggctgttcag
     8221 gctgcggctg ataggcaggc tgctgcacgg gttgctgcac cggctgcgct ggacgcggag
     8281 catgttgcgg cggtacctgc gcttcaggcg gctgctggac cggttgacgc ggctgcgcag
     8341 acgcataagg cggttggtac tggtgttgcg gcgacggacg agcagcctca tgctcctggg
     8401 cgttagccgg ggcatgattc acgcggtgaa cacgaacctc accaacgccc tcatcatctt
     8461 cgacatcctc gtcataagaa tcgtcgtcac gttttgactt cattcgtttt aatggccgat
     8521 cgcggaacat agaagatcgt tctttacggc tggtccagaa accatgtacc agtaaagcga
     8581 ttatggcgat cgcgccaaca atgattaata tcagacgcaa atcctgcatc attatattct
     8641 ctgttgttct aacaccttgc caccacggca aacatttact cactaagagt atttgccgat
     8701 tacctcaagt gcaagtgcac tattaacttt cacagcacaa agatagatga aatcgtgctt
     8761 tttgctgttt tttcgaacat atcctaactg tccattgcgc aattacccgg taaaatacgc
     8821 agaattttcc tgggattggt caaaaggagc tcatcctgat tatggtttca tcattcacat
     8881 ctgccccacg cagcggtttt tactattttg cgcaaggctg gaagctcgtc tcgcaacctg
     8941 ggattcggcg tttcgttatt ttaccgctgc tggtcaatat tttgttgatg gggggcgcat
     9001 tctggtggct ctttacacag ctcgatgtct ggatcccgac tctcatgagt tacgttccgg
     9061 actggctaca atggctgagt tatctgttgt ggcctctggc tgtcatctct gtgctgttag
     9121 tgtttggcta tttcttctca acgattgcta actggattgc cgctccgttc aacggtttat
     9181 tggctgaaca actggaagca cgattgactg gcgctacacc gccagatacc gggattttcg
     9241 gtatcatgaa agatgtgccg cgaatcatga aacgcgaatg gcaaaaattt gcctggtatc
     9301 tgccacgcgc aattgtattg ctaattcttt acttcatccc tggtattggg caaaccgtcg
     9361 cgccggtact gtggttcctg tttagcgcct ggatgttagc catccagtat tgcgattacc
     9421 ccttcgataa ccacaaagtg ccgtttaaag agatgcgcac cgccctgcgc acacgcaaaa
     9481 tcaccaatat gcagtttggt gctttaacca gtctgtttac gatgatcccg ctgcttaatc
     9541 tgttcattat gcccgttgcc gtttgtggcg cgacggcgat gtgggtcgat tgctatcgcg
     9601 ataaacacgc gatgtggcgg taacaatcta ccggttattt tgtaaaccgt ttgtgtgaaa
     9661 caggggtggc ttatgccgcc ccttattcca tcttgcatgt cattatttcc cttctgcata
     9721 tagatatgct aaatccttac ttccgcatat tctctgagcg ggtatgctac ctgttgtatc
     9781 ccaatttcat acagttaagg acaggccatg agtaagattt ttgaagataa ctcgctgact
     9841 atcggtcaca cgccgctggt tcgcctgaat cgcatcggta acggacgcat tctggcgaag
     9901 gtggaatctc gtaaccccag cttcagcgtt aagtgccgta tcggtgccaa catgatttgg
     9961 gatgccgaaa agcgcggcgt gctgaaacca ggcgttgaac tggttgaacc gaccagcggt
    10021 aataccggga ttgcactggc ctatgtagct gccgctcgcg gttacaaact caccctgacc
    10081 atgccagaaa ccatgagtat tgaacgccgc aagctgctga aagcgttagg tgcaaacctg
    10141 gtgctgacgg aaggtgctaa aggcatgaaa ggcgcaatcc aaaaagcaga agaaattgtc
    10201 gccagcaatc cagagaaata cctgctgctg caacaattca gcaatccggc aaaccctgaa
    10261 attcacgaaa aaaccaccgg tccggagatc tgggaagata ccgacggtca ggttgatgta
    10321 tttattgctg gcgttgggac tggcggtacg ctgactggcg tcagccgcta cattaaaggc
    10381 accaaaggca agaccgatct tatctctgtc gccgttgagc caaccgattc tccagttatc
    10441 gcccaggcgc tggcaggtga agagattaaa cctggcccgc ataaaattca gggtattggc
    10501 gctggtttta tcccggctaa cctcgatctc aagctggtcg ataaagtcat tggcatcacc
    10561 aatgaagaag ccatttctac cgcccgtcgt ctaatggaag aagaaggtat tcttgcaggt
    10621 atctcttctg gagcagctgt tgccgcggcg ttgaaactac aagaagatga aagctttacc
    10681 aacaagaata ttgtggttat tctaccatca tcgggtgagc gttatttaag caccgcattg
    10741 tttgccgatc tcttcactga gaaagaattg caacagtaat gccagcttgt taaaaatgcg
    10801 taaaaaagca cctttttagg tgcttttttg tggcctgctt caaactttcg cccctcctgg
    10861 cattgattca gcctgtcgga actggtattt aaccagacta attattttga tgcgcgaaat
    10921 taatcgttac aggaaaagcc aaagctgaat cgattttatg atttggttca attcttcctt
    10981 tagcggcata atgtttaatg acgtacgaaa cgtcagcggt caacacccgc cagcaatgga
    11041 ctgtattgcg ctcttcgtgc gtcgcgtctg ttaaaaactg gcgctaacaa tacaggctaa
    11101 agtcgaaccg ccaggctaga ctttagttcc acaacactaa acctataagt tggggaaata
    11161 caatgttcca gcaagaagtt accattaccg ctccgaacgg tctgcacacc cgccctgctg
    11221 cccagtttgt aaaagaagct aagggcttca cttctgaaat tactgtgact tccaacggca
    11281 aaagcgccag cgcgaaaagc ctgtttaaac tgcagactct gggcctgact caaggtaccg
    11341 ttgtgactat ctccgcagaa ggcgaagacg agcagaaagc ggttgaacat ctggttaaac
    11401 tgatggcgga actcgagtaa tttcccgggt tcttttaaaa atcagtcaca agtaaggtag
    11461 ggttatgatt tcaggcattt tagcatcccc gggtatcgct ttcggtaaag ctctgcttct
    11521 gaaagaagac gaaattgtca ttgaccggaa aaaaatttct gccgaccagg ttgatcagga
    11581 agttgaacgt tttctgagcg gtcgtgccaa ggcatcagcc cagctggaaa cgatcaaaac
    11641 gaaagctggt gaaacgttcg gtgaagaaaa agaagccatc tttgaagggc atattatgct
    11701 gctcgaagat gaggagctgg agcaggaaat catagccctg attaaagata aacacatgac
    11761 agctgacgca gctgctcatg aagttatcga aggtcaggct tctgccctgg aagagctgga
    11821 tgatgaatac ctgaaagaac gtgcggctga cgtacgtgat ataggtaagc gcctgctgcg
    11881 caacatcctg ggcctgaaga ttatcgacct gagcgccatt caggatgaag tcattctggt
    11941 tgccgctgac ctgacgccgt ccgaaaccgc acagctgaac ctgaagaagg tgctgggttt
    12001 catcaccgac gcgggtggcc gtacttccca cacctctatc atggcgcgtt ctctggaact
    12061 gcctgctatc gtgggtaccg gtagcgtcac ctctcaggtg aaaaatgacg actatctgat
    12121 tctggatgcc gtaaataatc aggtttacgt caatccaacc aacgaagtta ttgataaaat
    12181 gcgcgctgtt caggagcagg tggcttctga aaaagcagag cttgctaaac tgaaagatct
    12241 gccagctatt acgctggacg gtcaccaggt agaagtgtgc gctaacattg gtacggttcg
    12301 tgacgttgaa ggtgcagagc gtaacggcgc tgaaggcgtt ggtctgtatc gtactgagtt
    12361 cctgttcatg gaccgcgacg cgctgcccac tgaagaagaa cagtttgctg cttacaaagc
    12421 agtggctgaa gcgtgtggct cgcaggcggt tatcgttcgt accatggaca tcggcggcga
    12481 caaagagctg ccatacatga acttcccgaa agaagagaac ccgttcctcg gctggcgcgc
    12541 tatccgtatc gcgatggatc gtaaagagat cctgcgcgat cagctccgcg ctatcctgcg
    12601 tgcctcggct ttcggtaaat tgcgcattat gttcccgatg atcatctctg ttgaagaagt
    12661 gcgtgcactg cgcaaagaga tcgaaatcta caaacaggaa ctgcgcgacg aaggtaaagc
    12721 gtttgacgag tcaattgaaa tcggcgtaat ggtggaaaca ccggctgccg caacaattgc
    12781 acgtcattta gccaaagaag ttgatttctt tagtatcggc accaatgatt taacgcagta
    12841 cactctggca gttgaccgtg gtaatgatat gatttcacac ctttaccaac caatgtcacc
    12901 gtccgtgctg aacttgatca agcaagttat tgatgcttct catgctgaag gcaaatggac
    12961 tggcatgtgt ggtgagcttg ctggcgatga acgtgctaca cttctgttgc tggggatggg
    13021 tctggacgaa ttctctatga gcgccatttc tatcccgcgc attaagaaga ttatccgtaa
    13081 cacgaacttc gaagatgcga aggtgttagc agagcaggct cttgctcaac cgacaacgga
    13141 cgagttaatg acgctggtta acaagttcat tgaagaaaaa acaatctgct aatccacgag
    13201 atgcggccca atttactgct taggagaaga tcatgggttt gttcgataaa ctgaaatctc
    13261 tggtttccga cgacaagaag gataccggaa ctattgagat cattgctccg ctctctggcg
    13321 agatcgtcaa tatcgaagac gtgccggatg tcgtttttgc ggaaaaaatc gttggtgatg
    13381 gtattgctat caaaccaaca ggtaacaaaa tggtcgcgcc tgtagacggt accattggta
    13441 aaatctttga aaccaatcac gcgttctcta tcgaatctga tagcggcgtt gaactgttcg
    13501 tccacttcgg tatcgacacc gttgaactga aaggcgaagg cttcaagcgt attgctgaag
    13561 aaggtcagcg cgtgaaagtt ggcgatactg tcattgaatt tgatctgccg ctactggaag
    13621 agaaagccaa gtctaccctg actccggttg ttatctccaa catggacgaa atcaaagaac
    13681 tgatcaaact gtccggtagc gtaaccgtgg gtgaaacccc ggttatccgc atcaagaagt
    13741 aattcttgcc gcagtgaaaa atggcgccca tcggcgccat tttttatgct tccgccagcg
    13801 gcggcaaaat caattcatcg ctctcatgct gctgggtgta gcgcatcact tccagtacgc
    13861 gcaaccccgc acggtgcacc gcatcgttta acgccttccc tttcagcaaa ccactgataa
    13921 gctgagcaca aaacaggtcg ccagtccctt tcaggtcggt ttttacccgt gaatgggaaa
    13981 tgacattcac gctgtcggca ctgaccacca ccacctgcat ctcctgattt tcttcattac
    14041 cggaggcgct ggtaatcacc acccatttta atgtgtctga aagcagactt tttgcggcag
    14101 caatggcact gtcgagatcg cggcaatttt taccggtcag gatttccagc tcaaagatat
    14161 tgggggtaat tccctgcgcc agcggcagta aatattgtcg atacgcctcg ggaaggtcag
    14221 gtttgacata aattccgcta tcaatatcgc caatcaccgg atcgaccatg atcaataggt
    14281 caggatggtc tttgcgtagc gcagtcagcc actcggcaag gattttgatt tgcgatgccg
    14341 ttcccatata gcccgtggtt acagcgcgca gctggcgcag cgcatcacgc tcctgaagcg
    14401 cacgcaaata accgctaaac cattcatccg gaatcgcacc accgtagaaa gtgtcataat
    14461 gcggcgtatt gctcagcaat accgtcggca cggcaaaaac attcagaccg ttctgtttga
    14521 tagcaggcac ggcaatgctg ttgcccacgc tgccgtaaac cacctgcgac tgcacggcga
    14581 cgatatccgc ctgcagcgcc ctgctcttat cgttaaacaa caacaaacta ctcatttaat
    14641 tttttctcct tgccgatgat cctcatcgta ttccaaccga aactttacct gattctggca
    14701 gtcaaatcgg ctatcacaaa acaaggataa ggtaattcaa tgaagaaaat catttgtctg
    14761 gtcattacac tactaatgac actccccgct tacgcgaagt taactgccca tgaagaagcc
    14821 cgcatcaacg ccatgctgga gggattagca cagaaaaagg atttgatatt tgtgcgcaac
    14881 ggtgatgaac atacctgcga tgaagcggtt tctcatctgc gtctgaagct gggcaatacc
    14941 cgtaaccgca ttgacactgc cgagcagttt attgataagg ttgcttcgtc gtcatcgatt
    15001 accgggaagc cgtatattgt gaagatcccc ggtaagagcg atgagaacgc acagcctttt
    15061 ttacatgcgc taattgcgca gacggataaa acggtgcctg cgcaataatc ttaaatcccc
    15121 gccccctggc taaaatgctc ttccccaaac accccggtgg aaaggtagcg atcgccacga
    15181 tcgcagatga tcgctaccac caccgcacca gggttagctt ttgccacccg cagtgctccg
    15241 gcaaccgcgc cgccggagct gacgccacag aatattcctt cccgcaccgc cagttcgcgc
    15301 atggtgtttt ccgcatcgcg ctgatgaata tccagcacct catccaccag agaagcgttg
    15361 aaaatccccg gcagatattc cgcaggccag cggcgaatgc ccggaatact gctgccttct
    15421 tccggttgca ggccaacaat ggtcaccggt ttggattgtt cgcgcataaa gcgtgaaacg
    15481 ccggtgatag tgccggtcgt ccccatactg gagacaaaat gagtgatgcg cccgccggtt
    15541 tgctgccaga tttccggccc agtggtggtg taatgcgcat aagggttatc gggattattg
    15601 aactgatcga gcagctttcc ttcgccacga ttcgccatct ccagcgccag atcgcgcgca
    15661 ccttccatgc cctgctcttt ggtgacaaga atcagttccg caccataagc acgcatcgcc
    15721 gcacggcgtt cctggctcat gttgtcgggc atcagcaatt tcatgcgata gcctttaagc
    15781 gcggcaatca ttgccagcgc aatgccggtg ttaccactgg tggcttcgat taacacatca
    15841 cccggtttaa tttccccgcg cttttccgcc tcgacgatca tcgaaagcgc cgcacgatct
    15901 ttcaccgaac ctgccgggtt attgccttcc agttttaacc acacttcact gccgttattc
    15961 ggccccattc gctgcaactt caccagaggc gtattgccta ttgtttgttc taatgtactc
    16021 acgatctctg tccatacgtg gtgttgcctg atgcgacgct tgccgcgtct tatcaggcct
    16081 acaggttaca aaccttgcca ttaaaaagcc cggatcgcgg gaagcgcctc cgggcgttta
    16141 acattcactc aacctatcag gcgctttgtg cgagagcaag ttcctcatcg cgggtttcga
    16201 tacgctcgtc gccgttatac agccgcgcat gttgcagacc aacgaataaa cgctcgccac
    16261 gctgcggggc atcgtcgcca tgcatcacga ccgtcagcgg ttcgttgtac caccccagcg
    16321 gctgcaccac taattgggtg tagtgacctt tcgggctggc ttccagtacc tgtaccggca
    16381 gcggcgaatc gagactggta cggcggctga tatccacttc ccacgggcgc aggaagagat
    16441 ccaccggccc ctgataagca ggtgtgtagc ccagcggcca gcgatgtgcg ccaacatgga
    16501 actgcccgcc gcgaatggtt ccctgcaggc ggttcacttc gcccataaat tcgagcacaa
    16561 aacgggtcgc tggttcgcgc catacctgct ccggcgcatc agcctgttca atattgccct
    16621 ggctcatcac cactacacga tcagctactt ccgtcgcttc ttcctggtcg tgggtcacga
    16681 acacgctggt gaatttcagc tcttcatgca gttgacgcag ccagcgacgc agctctttac
    16741 gcacctgcgc atccagcgcg ccaaacggtt catcaagcag cagaatttgc ggttctacag
    16801 ccagcgcgcg cgccagcgcc acgcgctgtt tctggccgcc ggaaagctgt gccggataac
    16861 gatctgccag atgagcaagc tgcaccatct ccagcaattt tgtcactttc gctttgatgg
    16921 ctgcggcatt cgggcgctcg cgacgcggca gcaccgtcag gccaaaagcg atattgtcga
    16981 ataccgtcat atggcggaac agcgcgtaat gctggaacac gaaaccgact ttacgatcgc
    17041 gcgcgtgcag gcggctcacg tcggtgccgt ggaagcgaat atgcccgctg gtttgatgct
    17101 ccagcccggc gataatgcgc agcagcgtgg ttttcccgga accggacggc cccagcaacg
    17161 cgaccatctg acctgaagga atatccagtg agatatcgtt cagcacctgg gtgcgaccaa
    17221 aagacttctt aatattggca atctcaatgc tcatgatgtt cctcctgctg cgcgcgtttt
    17281 tcctgattct ccaggcgcca ctgcaacata ctctttaaaa acagggtgat aatcgccatc
    17341 agcgtcaaca gcgcggcggc agtaaaggag ccgacggtgt tgtagtcctg ctccagcaat
    17401 tcaatctgta acggcagcga aagtgtttcg ccgcgaatcg agccggaaac caccgacacc
    17461 gcgccaaact cgccaatcgc gcgggcgttg gtcagcacta cgccataaag cagcgcccag
    17521 cggatgttcg gcaatgtgac acgacggaac atctgccagc cagacgcgcc aagcaaaatc
    17581 gccgcctcgt cttcctgact gccctggctt aacatcaccg gcaccagttc gcgcaccaca
    17641 aacggacacg tcacgaagat ggtgaccagc accattcccg gccaggagaa cataatttgc
    17701 agattatgtt catccagcca accgcccagc gggccgttag agccgtagaa cagcagatac
    17761 accagaccgg caaccaccgg cgatacggca aacggaatgt ccagtagcgt cagcagtaac
    17821 tggcgtccag ggaagttaaa gcgcgtcacc agccaggcca gcagaatgcc gaacaccagg
    17881 tttaccggta cggcaatcag cgcgatcatc accgtcagcc agatggcgtg cagcatatcc
    17941 ggatcggcca gattctgtaa aaccggcatc agccctttgc tgaacgcctg tacgaagata
    18001 taaatcatcg gcaccagcag gatgaacgcc gaaaccagca tcccgatgcc aattagaaac
    18061 catttgcccc agttaatcgg gcgcgcgtca taacgcttca attgggtaac ttccgccatt
    18121 aatgacctac cacacgccga ccaaagcgac tttgcagagt gttaattgag aacagcagca
    18181 gcagagatgc cgcgaggatc accgaagcaa tcgcgctcgc cgccgggtaa tcaaactcct
    18241 gcaagcgcac aaaaatcatc agcgacgtca cttccgtctt ccaggcgatg ttcccggcga
    18301 taaatatcac cgcgccaaac tcaccaagac tacgggtaaa cgacaacgcc acacccgcca
    18361 ccagcgccgg agaaagttcc ggcagcacca ctttgcagaa actctgccag cgcgttgcac
    18421 caagcgtttc cgccgcttct tcatattccg ggcctaactc ttccagcacc ggctgcacgg
    18481 tacgcaccac aaacggaatg ctggtaaagg ccatcgccac cgcaatccca agccaggtgt
    18541 aggtgacttt gatatcaaac ttcgccagcc attcaccgta aaaaccgttc acggaaaaga
    18601 gcgaggccag cgttaaaccg gcgactgccg ttggcagcgc aaagggtaaa tccatcagcg
    18661 catcaagcag cgtgcggcca gggaagcgat aacgagttaa gatccacgcc atcagcagac
    18721 cgaaaacgcc gttaaaaatc gatgccacaa acgccgacag cagcgtcact ttgtaggctg
    18781 cgaccacctg cgggttggtg atcacctccc agtactgcgc ccagctcatc tgggccagct
    18841 gcatcaccag cgcggagagt ggcaggagca aaatcaggca aacaaacagc agactggtgc
    18901 cgaggcttaa ggtaaagccc ggcagcacgc gtcttgatga aactgcaaac attaattacg
    18961 ccccgccgct aacagcttgt ctaactcgcc gccgctggtg aagtgggttt tcatcacttc
    19021 cggccaggag ccaaatttgt cttccacgcg gaacagctcg gtctgcggga atttgttttt
    19081 cagtttgtcc atgacttccg ggttatttac gcggtagtaa tagtcggtga tgatggtttg
    19141 cgcctgcggg ctatagagcc agttcagata ggctttggcg gctttttccg taccgttggc
    19201 ctgcacgttt ttatcaaccc acgccaccgg gaattccgcc agaatgttgg ttttcggaat
    19261 caccacttca aagccctgcg cttcatactg tttacggatg ttgttcactt ccgattcgaa
    19321 gctaatcagc acatcgccca ggccgcgctc ggcaaaagtg gtggtcgcgc cacgaccgcc
    19381 agtatcgaac acttcaacgt ttttcaggaa ctgggtcata aactgttcgg ttttgccttt
    19441 gtcaccaccg tcagctttat ccgctgcgcc ccatgccgcc agataggtat aacgcgcgtt
    19501 acccgacgtt ttcgggttcg ggaaaatcag cttcacgtcg gagcgcacca ggtcgttcca
    19561 atcgtggata ttcttcgggt tacccttacg caccaggaag cccatggtgg agtagaacgg
    19621 cgagctatta ttcggcaggc gcgactgcca gtcggccggg atcagcttgc ctttatcgtg
    19681 caggatttgt acgtcggtca cctggttata agtgacaacg tcggctttta agccctgtaa
    19741 aatcgccagc gcctgttttg atgacccggc atgagattgt tttatcgtca gtttgtcgcc
    19801 gccgttatct tttgcccatt gttgctcaaa cggcggattc agggcggcaa acagctcgcg
    19861 ggagacgtca taagaactgt tcagcagttc cgttgcctgt acatggcccg ccagcagcag
    19921 agaagcgacc agcgcgagtg agttcttttt cagtaagtta acggccattg cgcaccctta
    19981 taaatttaat gactttctaa tagccatcat atttataacg gacgtgaaag gagtaacggt
    20041 tttatatacc gtttggtgat ttggaagttg aaaagggaat aagaaccggg tttaaaacga
    20101 aatcctcccc acaaatgcag ggaggaaaca gaggtgaatc agataccgac gctaaccgtc
    20161 tccggcagtg tgctgccgcc atcaatcaca ttctgtgtac cggttaaata gctggattca
    20221 tccgatgcga ggaaggccgc cagttcgccg acttccagcg gatcggcgag gcgacgcatc
    20281 gggattgctt tcgccatttc agtcagcacc gactctggat cttccgggtt cgactggcgg
    20341 gcaatgcttt ccgccattgg tgtgcgcacg tatcccgggc aaatggcgtt aacgcgaata
    20401 ccagactgcg cgtactccac cgccagcgat tttgtcaggc caacaatcgc cgctttcgtt
    20461 aaggcgtacg ccgtttcgcc aggatcggcc accatatcgc cagtgaccga agacatcatc
    20521 acaatgcgac catctttgcg ggcaatcatc tccggcagca ccgccttcgt gacgttccat
    20581 acgcctttaa tattgatgtc aatatggaaa tcgcgatcgt catcgctcat atcgaggaaa
    20641 ctgcccagac gacaaacgcc tgcgttattc accaggatat caatgcgccc ttctttttcc
    20701 ttcgcgcgtt tgatagctgc ggctaccgac gccgggtcac gcacatcggc gacaaccgcc
    20761 gtacagcgat gaccacgacc acacagttcg tccgccagct tttcgatctc aggggagata
    20821 tccagcaaga ttaggtttgc gccatgacgc gcaaaagttc tggcaattcc ttcgccaatt
    20881 ccctgcaatg cgcccgtaat cagtgctgtc ttgcccgtga gtttacccat tttaatgctc
    20941 tccttgttaa tacagagcgt tacactccat tccctctgaa aatacaggat aaacaaaata
    21001 ttgctgcgga tcagctcact aattcaggaa aatctcaatc cagtcgctgc tttatgcatc
    21061 atttcaggcg ttgtgttagc tcactgctgc gctgaatcat tttcaatgac tccacatcat
    21121 tgagttgcac aagaccaaca aacagtaaat cggtaacaga attttgcgcc gtgcgggtag
    21181 acatcgacga gctacgccat tcggtttcac cggaaacggt atcgagcgtg aaatgcgcca
    21241 gccgccgcag cggagaatca gccagagaag taatggcaat caccgttgcc ccctgctttc
    21301 gtgccgcttc tgcgcataag acaatctctt ttttactgcc gctgtaagag atagcgattt
    21361 gtacatcgcc ttttttcaac gcctgggaaa cggtggcttg tacgtgcgtg tcggcttcgc
    21421 aggccacgcg atagccaatt ttcatcagtt tgaacgacag atcgcgcccc accagcgccg
    21481 atccgcccag gcccgttatc tggataaacg gtgctttact gatgacctca atgatttttt
    21541 gcagccgcgc gtagtcgaac aacgcacagg tttgctccag cgccagctct ttttcgcgat
    21601 tcaatttgcg ggcaatcact tccagcgaat catcactggt aatcgaactg tgcaggtgca
    21661 gcgccgtggc attcgttttc tcccggctgg cactgtattc gcctattaac gccatacgca
    21721 attcagtaaa tccttgcgcg ccgagttttt gggcaaattt cactatgctc gactggctaa
    21781 tgccgagctg tttcgccatc tgccgtgaag aaacggattg cagctcactc acgttggcct
    21841 gcagaaagtc agcgattttt tgctcgtttt cggtgaattc actccccgca ttgcttattt
    21901 tcgtcaggta caacatcttc cactctcctg ggctggcagc ataaattccg cttaagttct
    21961 tatgacgctc ttcacactct gcgagtgtag actcaatgat tcctttaagt ctgtcaattc
    22021 gggatatatg attccataca tctgcaatta tttgatcgta aatagtaagg tcaccaccga
    22081 tgcaacttga aaagatgatt accgaaggct cgaacaccgc ctcggctgaa attgaccgcg
    22141 tatcgacgct ggaaatgtgc cggattatca acgatgaaga taaaaccgta ccgcttgccg
    22201 ttgagcgcgt cctgccggat atcgccgcgg cgatcgatgt tatccacgcc caggtcagcg
    22261 gcggcggtcg tctgatttat ctcggtgcgg gaacatccgg tcgtctgggg attctggatg
    22321 ccagcgaatg tccgcccacc tacggcgtga aaccgggtct ggtggttggt ttgattgctg
    22381 gaggcgaata tgccattcag cacgcggtgg aaggcgctga agatagccgg gaaggtggtg
    22441 ttaacgatct gaaaaatatt aatttaacgg cacaggatgt ggtggttggc attgctgcca
    22501 gcggtcgcac gccgtatgtg attgccggac tggaatacgc acgccagctc ggctgccgca
    22561 cagtgggaat ttcctgtaat ccggggagtg ccgtttccac caccgccgag tttgccatta
    22621 caccgattgt aggtgccgaa gtggtcactg gttcttcgcg aatgaaagca ggcacagcgc
    22681 agaaactggt gctcaatatg ctttccaccg ggctgatgat taaatctggc aaagtgttcg
    22741 gcaacctgat ggtcgatgtg gtcgccacca acgaaaaact gcatgtgcgc caggtcaata
    22801 tcgttaaaaa cgccaccgga tgtaatgccg aacaagcgga agcggcgtta attgcctgcg
    22861 agcgcaactg taaaacggcc attgtgatgg tgctgaaaaa tctcgatgcc gcagaagcta
    22921 aaaaacgcct ggatcaacac ggcggcttta ttcgtcaggt tttagacaag gaataaccca
    22981 tggccaaaga gatcagcagt gaacttctga acaccatcct tacccgtgtc ggcggaccgg
    23041 gaaatatcgc cagttgtggt aactgtatga cgcgcctgcg tctgggtgta catgacagtt
    23101 cactggttga tcccaatatc aaaacgctgg aaggcgtgaa gggcgtcatt ttgaccagcg
    23161 accaggtgca ggtcgttttt gggcctggta aagcgcatcg tgccgcaaaa gcgatgagcg
    23221 agctgctggg cgaggcaccg gtacaggatg ccgcagaaat cgccgcccag aacaaacgtc
    23281 agttaaaagc caaacaaacc tccggcgtgc aacaatttct cgccaaattc gccaccatct
    23341 tcacgccgct gatccccggt tttattgccg ccggtctgct gctggggata gcgacgttaa
    23401 ttgccacggt gatgcacgtt ccggcagatg ctcagggcac gctacccgac gcgctgaatt
    23461 ttatgaaggt gttcagcaaa ggtttgttca ccttcctggt gattctggtg ggctataacg
    23521 ccgcccaggc atttggcggc acgggcgtca atggcgcaat tattgccgca ctctttttgc
    23581 tcggttataa ccccgctgca accaccggtt actacgccgg ttttcacgat ttctttggtc
    23641 tgcccatcga tccgcgcggc aatattatcg gcgtgctgat tgccgcgtgg gcctgcgccc
    23701 gcattgaagg catggtgcgc cgctttatgc cggacgatct cgacatgctg ctcacctcgt
    23761 taatcaccct gctgatcacc gctacgcttg cgtacctgat cattatgccg ctgggtggct
    23821 ggttattcga aggtatgtca tggctgttta tgcacctgaa cagtaatccg ttctgttgtg
    23881 cggttttagc cgggctgttc ctgatcgccg tggtatttgg cgtgcatcag ggctttattc
    23941 ctgtttacct cgcgttaatg gacagccagg gattcaacag cttatttccc atcctttcaa
    24001 tggcaggcgc gggccaggtg ggcgcggcgc tggcactcta ctggcgggcg caaccgcaca
    24061 gtgcattacg cagtcaggta cgcggggcga ttattcccgg tctgctgggc gttggtgaac
    24121 cgttgattta cggcgtcacc ctgccccgca tgaaaccgtt tattaccgcc tgtttaggcg
    24181 gcgcagcggg cggtttgttt atcggcctga tagcctggtg gggtctgccg atgggcttaa
    24241 acagcgcctt tgggccgtct ggtctggtgg cgctgccgct gatgacttcc gcacaaggca
    24301 tccttccggc aatggcggtt tatgctggcg ggattctggt ggcatgggtt tgcgggttta
    24361 ttttcaccac gctctttggc tgccgtaacg tcaatctgga ctgatatcat gaaacggaca
    24421 atgctctatc tttctctgct ggctgttagt tgtagcgtca gcgccgcaaa ataccctgtt
    24481 ctgacagaaa gttcgccaga gaaagcaggg ttcaacgtcg aacggcttaa ccagatggat
    24541 cgctggatta gccagcaagt ggacgccggt tatcccggcg taaacctgct gatcattaaa
    24601 gataatcaga ttgtgtatcg caaagcctgg ggagcggcga aaaagtacga tggcagcgtg
    24661 ttgatggaac agcctgtcaa agccaccacc gggacgctgt atgacctggc ctcaaacacc
    24721 aaaatgtacg ccacgaactt cgccctgcaa aagctgatga gcgaaggcaa actgcatcct
    24781 gacgatcgga ttgcgaaata tattccgggg tttgccgata gtccaaatga caccattaaa
    24841 ggcaaaaaca ccctgcggat ttctgacctg ctgcatcaca gcggcggttt cccggcagat
    24901 ccgcaatacc cgaataaagc ggtcgcgggc gcgttatatt cccaggataa aggccagacg
    24961 ctggagatga tcaagcgcac gccgctagag tatcagcccg gcagcaagca tatttacagt
    25021 gatgtcgatt atatgctgct tggatttatc gtcgagtcgg tcaccggtca gccgcttgac
    25081 cgctatgttg aagagtcgat ttatcgcccg ctcggcctga cacatacggt atttaacccg
    25141 ctgctgaaag gctttaaacc gcaacaaatt gccgccacgg aattgaacgg caatacccgc
    25201 gatggcgtga tccatttccc gaatatccgc acctccactc tctggggtca ggtgcacgat
    25261 gaaaaagcct tttattcgat gggcggcgtt tccgggcacg caggtttgtt ttccaatacc
    25321 ggcgatattg cggtgttaat gcaaacgatg ctgaacggcg ggggctatgg cgatgtgcag
    25381 ctgttcagtg cggaaacggt gaagatgttc accaccagct ctaaggaaga tgccactttt
    25441 ggcctcggct ggcgcgtgaa tggtaatgcc accatgacgc cgacgtttgg cacgctggca
    25501 agcccgcaaa cctacggtca cactggctgg accggaacgg tgaccgttat cgatccggtg
    25561 aatcatatgg cgattgtgat gttaagcaac aagccacatt cgccggttgc cgatccgcaa
    25621 aagaatccca atatgttcga aagcggtcag ctgccgattg caacgtatgg ttgggtagtt
    25681 gatcaggtgt atgcggcgtt aaagcaaaag taataaaaag gccagtcagt aatgttactg
    25741 cctggccttt aatcaatgaa tcagaaacgc ttacagcgcc atcaacttgt ccagcgacgg
    25801 tgcgaaataa tagccgccgg ttaccggttt ggtgaaacgc aacatcgcat cacgcttacc
    25861 atcggtatcg ccaaacatgc tcagcagttg ctgctcaatg ttatgcagac gcgcgcagta
    25921 ggcgcagaag tacagaccgt gagtgccact ggcggtgccg tacggcaggc tctggcgaac
    25981 aatcttcagc cctttgccat cttctttcag atcaacgcgg gtgaggtgag aggtttccgg
    26041 acgttcgtcg ccgtcaatct cttcgttggc ctctttggtg cgcccgatca tcatctcctg
    26101 atcgtgaacg ctcatccggt tgagctgctt caggttgtgc tcccaacgct gcacaaacac
    26161 atagctgccg ccggcatcca cgccgtcttt gataaccgcc acttcgcgac gcgtctcttc
    26221 acccgccggg ttttctgtac cgtcaacaaa gccgctcagg tcacgctctt ccacccaacg
    26281 gaagccgtgg atctcttctt tcacttcaat gcagtcacca aaggcttcca tcgccgcctg
    26341 ggcgacagag aagtttacgt cgtgacgcag agaaagaatg tggatcaaca catcgaactg
    26401 ggtggtcggc gcaaggcctt taccgtagcc cggaaaatct ttcagctctt ctgccccaac
    26461 gccgccgctc agagcgcgcc aggtgttgtt accaaaggca accaccgcac caagatgcgc
    26521 gtccgggaat ttcgcttcaa aagttgccag tttgtcggca aatgttttac tggccgcacg
    26581 cagggcgtca acttcccctt tcacgttggc ttcgatccaa atcgccgcgc ggcaatgttc
    26641 tggcaaaatg ccactctgaa cctgagacat tgttcctcct gaaaataaga atgccacgca
    26701 atccgtggca ttgttgagcg ctattttacg tgttttttcg cggcggcggt ttgttcagac
    26761 gcaaattaac gccgccagat aattttactg actttccagt ttttcagggt atcgtccgaa
    26821 ggcattaacc cttcaggacc actccattcc ccgctgaact ggtagctaat atgttgactg
    26881 ccttcggctt tacattcgac ggcacgatta tcatcaccat cagctttttg gcaattgcca
    26941 aatgctttgc tgtaaaggtc gctaaacggt gtaccgattt taacaccggt gtcagcagga
    27001 atatcgctat ccagcacgtc aatgcgactg atcgtgcctt gatcgccgtt aatcaccatc
    27061 gccacgttgt cgcccttcat cacttcaaaa aagcgcacca cgttgccgtt cgcggttttc
    27121 attccgctgc gcaggcgata atcaccatca agcgcatcgg caatggcttg ttcttgcagt
    27181 ggtgtggacg ccgttaattc accaacgccc tgctcgctca ctttggtgga cgacccaaac
    27241 cagttccacg ggttagcggc agaccagtta actgacgaca tcgtggaaca gccggtcagc
    27301 atcaacggca tagcgcataa cattaaacgc agcgatttca tgtcacttcc tttggttatt
    27361 caataacgtt gcttggagtg caaattcacc aaaaagtgcc gttaaaggtt gatgacaaac
    27421 acaaaattgc ctgatgcgct acgcttatca ggccttcttt ctcctgcaat atattgaatt
    27481 ttcgatgttt tgtaggccgg ataaggcgtt cacgccgcat ccggcataaa caaagcgcac
    27541 ctctgcgtta ttctttttct tcattaaaac aagcgcgtaa acgtcgattg gtcagcagcc
    27601 agattaacgc cacaatatcc gccactacca gcgccagacc gaaaccgcta acggattcac
    27661 cgttcagcca cagccacggt tgccagcaaa gtaaaaccac ctgcgccaac agcaacagaa
    27721 aatagagtac acgccaggtg cgaggaaacg tagcccgccg accgctcaac aaaaacgcca
    27781 gcaccgccgg aatgccagga atcagcccca gccagaaatt atcgtgatcg ggataaaaca
    27841 gatttagcag cgcagtaccc tgctcacgcg acgcaccggc aatgacaaac agcacccagg
    27901 ttcgcgcctg aagcaatagc acaagccaga agagcaaggg taaacgcagg cgtccgtgcg
    27961 catcataatg gacaggatgg aactcagtac tcttcatctt caatcaaacg cttacccaga
    28021 ctcagcacgt cggcgtgttc atatcccagg cgttcataca ttccgagcac catgtcgtta
    28081 tcttccggca cgttgatctg aattttcggg cagccgcgag caatcagctt tttctccagc
    28141 cggttaagca acgcattggc aattccacgc ccacgaaact ctggatgcac gccaagataa
    28201 taagcagacc cgcgatgccc gtcataaccg cccatcaccg ttccgaccac ttcaccgttt
    28261 acctcagcga ccagaaacaa actgacgtca tggttcatct tacgctcgat gtccatttcc
    28321 ggatcgttcc acggacgcag caagtcgcaa cgctcccaaa gggtgatgac ctcttcgaaa
    28381 tcttcctggc gaaatacgcg tatctccatg gtattcgtta cctttttgcg ggttaaaagg
    28441 ctgattatgg cgtgaacggt cgaattagcc aatatctgac gaaaatcggt tgaaaaagtg
    28501 gcataatggg gagttgtcaa ctattgaaat gaaaagtaaa acaattctca acagcaaacg
    28561 gtcgtaacgg attacgcgat acgatataac atctggaact ttattattac aactcaggcc
    28621 gtatgagcac ttttaaacca ctaaaaacac tcacttcgcg ccgccaggtg ctgaaagccg
    28681 gtttggctgc cctgacgttg tcaggaatgt cgcaagccat cgccaaagac gaacctttaa
    28741 aaaccagcaa cggacacagc aagccgaaag ccaaaaaatc tggcggcaaa cgtgtcgttg
    28801 ttctcgatcc aggccacggc ggaattgata ccggagcgat cggacgcaac ggttcgaaag
    28861 aaaaacatgt ggtgctggcg attgctaaaa acgtccgttc cattttgcgt aatcatggga
    28921 ttgatgcgcg tttaacgcgt tctggcgata cgtttatccc actttacgat cgcgttgaaa
    28981 tcgcccataa acatggcgca gatctgttta tgtcaattca tgccgatggc tttaccaacc
    29041 cgaaagctgc cggtgcttcg gtatttgccc tctctaaccg tggggcaagt agcgcaatgg
    29101 cgaaatacct gtctgaacgc gaaaaccgcg ccgatgaagt tgccggtaaa aaggcgactg
    29161 acaaggatca cctattgcag caagtgctgt ttgatctggt gcaaaccgat actattaaaa
    29221 acagcctgac gcttggctcg catattctga aaaagattaa gccggtgcat aaactgcaca
    29281 gccgcaacac cgaacaagcc gcgtttgtgg tgttgaaatc gccgtcggtt ccttcggtgc
    29341 tggtggaaac ctcgtttatc accaacccgg aagaagagcg gctgttaggc acggcggcgt
    29401 ttcgtcagaa aatcgccaca gcgattgctg aaggcgtgat cagttatttc cactggttcg
    29461 acaaccagaa agcacattcg aaaaagcgat aagttatgaa acccgacgca caccaggtta
    29521 aacagtttct gctcaacctt caggatacga tttgtcagca gctgaccgcc gtcgatggcg
    29581 cagaatttgt cgaagatagt tggcagcgcg aagctggcgg cggcgggcgt agtcgggtgt
    29641 tgcgtaatgg tggtgttttc gaacaggcgg gcgtcaactt ttcacatgtc catggtgagg
    29701 cgatgcctgc ttccgccacc gctcatcgcc cggaacttgc cgggcgcagt ttcgaggcga
    29761 tgggcgtttc actggtagtg catccgcata acccgtatgt tcccaccagc cacgcgaatg
    29821 tgcgtttttt tattgccgaa aaaccgggtg cagagcccgt ctggtggttt ggcggcggct
    29881 tcgatttaac ccctttctat ggttttgaag aagacgccat tcactggcac cgcaccgccc
    29941 gtgacctgtg cctgccattt ggtgaagacg tttatccccg ttacaaaaag tggtgcgacg
    30001 attacttcta cctcaaacat cgcaacgaac agcgcggtat tggcgggctg ttctttgatg
    30061 atctgaacac gccagatttc gaccactgtt ttgcctttat gcaggcggta ggcaaaggct
    30121 acaccgacgc ttatttacca attgtagagc gacgtaaagc gatggcctac ggcgagcgcg
    30181 agcgcaattt tcagctctac cgtcgcggtc gttatgtcga gttcaatctg gtctgggatc
    30241 gcggcactct gtttggcctg caaactggcg ggcgcaccga gtcgatcctg atgtcaatgc
    30301 cgccactggt acgctgggag tatgattatc agccaaaaga tggcagccca gaagcggcgt
    30361 taagtgagtt tattaaggtc agggattggg tgtaactccc tcacccccat tcccgcatcc
    30421 gctgatgcaa cgtcaacgac ggcttctcgg caaacagctg ctggtaatcc gtggcaaatt
    30481 gccccagatg ccagaatccc cactgcatgg cggcgtcttt taccgttgtg ctttgcgacc
    30541 acgggcttat cagttcgcgg cgtacggcgt tcaggcgaat gcgtttcagc cacgcgtttg
    30601 ggccaatgcc taaaatagcg tgaaacgcgt tttgtagcgt gcggcggctg acatgcagtt
    30661 gattacacaa atccagcacc gtcaccggtt cggacatgtt ttccagcaca tattcacggg
    30721 cgcgggaaag taatctacgg taactctgat gactgatgct ttccgccgtc accattggtt
    30781 gcgcttcttc cagcatcgcc cccatcgcca ttagcaaatt atcccccagc acttttcgca
    30841 ctgcaggctg atgaagattt tccgggttct cgcaaaacgt cgccagcgcc tgttggacaa
    30901 agccccacag cgcggcttta tgctgctctt tcacttccag cgccgactgg ctacgcaaca
    30961 tatgtaatac ccgatccgga ttatgcaaaa agttagcctg acgggtgatg acatcttcag
    31021 aaagcactac gcccaggatc gtgtaatcgt ccggcgtact taattcaaac tcggttccac
    31081 cagggcgcgt cgctatttct gcgcttccca gacattgcga accgataaat ccctgctcac
    31141 cgcgcgtcgc cggaatgcca aaccagaacg agttcggcca gaccagacac gactgacgca
    31201 gcgccagacc ggtgtattca cgaaaaacct gaatatcatc gagtagaatt tccgtaaatt
    31261 caccatgaaa cttgcccgga tgcagctgat cgtaaatctg ctgccaggcg gtaatcgtta
    31321 aagcatgttc atagacatcc gttgtctgtc gttgatgaac attatccact tcgaccttcg
    31381 gcgtgagctt caggttttcg ggtaagggtt catgataaag atggtgcaaa ttggctgtac
    31441 gggtcttttt catgatgtta atgccgggtg ttgtaggaca cccggcacct ccgacaggtt
    31501 aatggggctt gagacgataa cgactactgc gtttacgtaa cgtcccggca gaaaagagct
    31561 gttcgagcgc atttctggct ttttccagcg gccagccaaa gtgggcagcc acttctcctg
    31621 ccgtcattcc ctgacgtact gatgttaaca gcgccaacaa ctcgccagca gattccgcaa
    31681 cgataactgg cgcgtcgggt tcctttacgg gttgcttcgg ctggcggtta aagccagtaa
    31741 ccagccactg ggtgtcatcg tctggccgac cgatctcctt acggctgatg acacgcccgg
    31801 tacgcatcgc ggcagcacaa cctgcgtcca gcgcagcacg acacgccgcc agatcgcctt
    31861 ccaccaccag cgttaagcga ccagggtcaa gcacttcgtg actgagcaga cgaacgttag
    31921 ctgctttgag catggcatcc gctgcatcta tcgcagcgac catgccgtcc acttccagca
    31981 atcccagtgc attgatcatc ggcaacctcc attacgcacg ctggattgga ttacgcgcga
    32041 tttccagcac tgcatcggta aaggcgttac aggctgcttt acacgctgcc tggctaccgg
    32101 ttaaaaatgc tgccgagtag ttggtttcag acggtggcgg gacataggtc accagctgta
    32161 cgtcggcaga cttcagggct gcatcaatgc cataggtcgc ttccagcggc ggtgccacca
    32221 gatacgccat cggatcgcca agcgtgatcc cggcggttga tgagagataa gaaccggtac
    32281 gcgaaactac atgtgccagg aatgccgtat cttgcgcatc attagcccac tggaaagcag
    32341 cgccattttc aatatgcgca accatcgcat ccagacctgc acgcacttcc gccgggtttg
    32401 gcccaccaag cataatcagc acttcaccgg cagtcggtga cgggccgtgt gcagcgcccg
    32461 catacagcga gcggccatac accacttcca ccatcgcctg cttggtcgct tcatcagcgg
    32521 caatgtaggt gacgtcatcg gagtcggcag aaatcagacc gaggctacga atatgcggcg
    32581 gcaatttcag ttcacgcgca aaatcggcgt taacggaggc aatcacccgc atagcggtga
    32641 cagacggtcg aatcaaatct aaagctggca tgatgcctcc ttatcgggtc atgttgatgc
    32701 cggatgcttt ctgctccagc atacgtttcg ccaaatccac aatgacggct gcggcttcaa
    32761 ccggcggcgt gccgccctgg tgaatgttag agatacaggt gcgatcggcc tcaacggtgg
    32821 tcgccatacg cggcgagtaa acggcgtagc aggagaggct ttctgactgc cctaaccccg
    32881 gacgttcccc caccagcagg atcaccactt tcgcgccgag gatctcaccg atctgatctt
    32941 caatcttcac gcgaccataa cgaacaaaga atggcgtgcc gactttcagc ccggcctgtt
    33001 tcaggcccgc catcagcggc ggcaggatct cttcgtagtt cacagtgatg gcatcagttg
    33061 acaggccatc agaaataacg acctgtacgt ccggattagc aacacactgt gctttcagcg
    33121 cttcaacagc ttctgcacac aggcggcggc ccatatccgg gcgagtcagg tagagatttt
    33181 tgtcgctaat ttcggagcgt acttccagta agccctgcgc tttcacccac tcttccggca
    33241 cttctttaag tacggtatct ttcgaacgag agtgatcggc caggaaacgc agcaacgcct
    33301 gggtacgcag acgcggaccg gcacgaccgg tacaaacgcg ggccacggtg ctgcgacgca
    33361 gttctgttaa cacatccgca cgatgtgggt tttcaacacc aatccacgct tttgcttccg
    33421 cagaacctaa atccagcgcg cagctttccg aggtcaccgg tgccgcacag gtggtagtgg
    33481 cgcactttgc ttctgacggc gcgggggccg tttgtcccat tgacgccatc acgctgcgta
    33541 caatttcttc aatctgtttt tgatccatga tgtgttatct ccgcgtcatc agaagaacag
    33601 tgacggatcg cccgcccgtt tggtcaggcg accgtttgcc ataatgccca tgctttccag
    33661 ccagcgttca aactccggtg acgggcgcag gttgagtaac tgacgcacag tggcggtatc
    33721 gtggaatgcg gtggtctggt agttgagcat gatgtcatca cccagcggca tccccatgat
    33781 gtagttgcag cctgcggtgg cgagcaggat catcaggttt tcgttgaggt tctggtcagc
    33841 gtcagcgtgg ttggtgtaac agcagtcaca gcccatagag atgccgctca gcttgcccat
    33901 aaagtgatct tctaagcccg cacggataat ctggcggtcg ttgtagagat actccggccc
    33961 aataaagccg accacggtgt tgacgataaa cggatcgtaa tgacgcgcca gcccatagtt
    34021 acgtgcttcc atcgtcacct ggtctgcgcc gaagttagcg ccagcggata gtgctgagcc
    34081 ttgtccggtt tcgaagtaga ggcagttttc cccggcgata cggttgaact ctgcgcccac
    34141 tgcgcgcgct tcgtcgagca tcgccagttc cacgccaaac tctttcagcc ctttttcgct
    34201 gccacagata ctctggaaaa tcagtccgcc aggcgcgccg cgacgaatcg cttcgatctg
    34261 ggtggtgacg tgcgccagta cgcagccctg agttgggatg ttgaatttgt cgatcacgcc
    34321 atagatggtg tccagcacgc ggcttaagtt ttccacgtcg tcagttaccg ggttgacgcc
    34381 gatcaccgca tcgcccaccc cgaaggaaag cccttcgtag atttgcgcgg cgatactttg
    34441 cacgtcgtca cgggtgtcat ttggctgcaa acgggcgctg aaggtgcccg gaataccgat
    34501 ggtggtattg gcctttttga ttaccggcat tttcttcgcg ccgtagatca ggtccgcgtt
    34561 ggagcaaatc ttcgctaccg ccgcgaccac ttccgaggtc agccctttgc gggtaaaggc
    34621 aatgtcgtcc acgctggttt catcgctcag cacatactca cgcagttcgc tgatgctcca
    34681 gtttttaatc tggttgtagg ccgtttcgtt aacatcgtcc tgaatcagcc gcgtcacgca
    34741 gtcatcttca taggcaatca ccggattatt gcggatgtcc gctacggtca tttccgacaa
    34801 cacctgcttt gccgccacgc gctcctgtga gcttgccgct gcgacgcccg ccagcacatc
    34861 ccccgaacgc agttcgttgg ctttagccag cacctctttt acatccttaa actgatatac
    34921 attgccgaac aatgtggtct ttagtttcat aagtcgttcc ctcaggaagg aaatgcgagt
    34981 gatttcaccg tcaccggcac aaccgatccg ccaaaaagag gcgtaccaat gtcgatatag
    35041 tcccccgcgc ggacaatcac ttcgtcaatg actgccaacg ggagttgttg tagctgtggg
    35101 cgcaacaaca tgcccagagc tttaccaaag tcctgcccgg ccaccaccag caggggatgc
    35161 ggattcggaa aacgcgcgac gaaatcgacc agcgcgttga tgaccgtcag taccgcggcg
    35221 taacgcacag gcagcgaggc gggaagcgcc agcacgtacg cgtcagtttt gggatcaaga
    35281 tccagctgaa gcagcgcctg ttgccaggca ctcaccagat ccgtttcatc aatcgggatc
    35341 gccaccggca aattgcgcag cggcagttgt acgccctcca gccagattgt gctgccagag
    35401 agcgaaaggg tatgtgcacc cgcgccaatc accgtggcgc gtacggtttg cgccggaaac
    35461 tgcacattca tctcacgcag gcgcggatgg tcatgcagcg ccgttgccag aagtgggcca
    35521 atatcggcaa aacagaacgg gtcggcgggc tggtggcgat aacattcgcc cacgcctcca
    35581 gaaagcgtaa tgatttcggg cgtaacacct gcgggcagca aaccggtttg catcaatgcc
    35641 tgcgcgagcg gcgaaagcgt tccgtcaatc acttcgacaa tcagcgccgc catccgccgg
    35701 gtaacctgca ccagctgcgc gccggtcagc gaacgggcgt cggtgcctgc gccgaagcac
    35761 tcatccacaa tcatctgccc cggtttatga gcgtaaacca cgcgcccctg gctgtcggtt
    35821 tccagcaggc gaccaccgac gttgaggcag gcagtgccgc tgatttttcc ggcatcgaac
    35881 agggcgtagt tcgcggtgcc accgccgatg tcgatattca gtacccgaca cagccgttgt
    35941 tcagaaaggg tttgtgcccc ggctccgtga ccggcgatca cggattcgag gtgcggcccg
    36001 gcgctggcaa cgacaaaatc gcccagcgac tgagagagcg ccatcaccgc cgggcgagca
    36061 ttgcgggttt tcgcgctttc accggtgatg atgatggcac cagaatcaac gctttccggc
    36121 gcaatgcccg cagcctgata ttgctctaaa atcagcgatt tcagttccgc ttcttttaaa
    36181 ccaccctgtt tatcgacagg ggtaaagaac accgggcttt gccagctaat ttcgcgtttg
    36241 atgaattcgt agcgcggcac ctgcgacacc gccgcacggt taaccagttc caggtgggag
    36301 aaaatcacct gggtggtggt ggtgccgata tcgataccga cgctcagtag ctggcgagtg
    36361 ttcacgattg tgcctccgct tcggttttag ctgcggtcgc gtcgtctttt ggcaccagca
    36421 tcatcgccac gccaatcgcc gttacgccgc cgatcaactt gccgacaatc atcgggaaga
    36481 tcatggcatt catgttggca gcggcgaacc ctaagtggtc gcccagggcg aaagcagcag
    36541 aaacggcgaa ggcgcagttg atgactttgc cgcgggtatc catctgcttc atcatgccga
    36601 acatcgggat gttgttggca agcgttgcca ccatgccggc tgccgcgatg ttgttcatat
    36661 tcagcacttt accgacgctc atcagcggtt tttcaaacca gcgggtcagc agcagcacca
    36721 tcggatacgc ccctaacaga acgcaggaga tagaaccgat aacttcaatg gcgcgcatca
    36781 cctcaccggg tttatcgcca ggggccataa agataggatc cagaccgggg atcagttccc
    36841 agccaagcag gaatttcact accgcagcgg caagaccgag ggtgatcaat gcaacgagga
    36901 atttggcgaa gatctggaag ccgttgatca ttttttccgg gatgaatttc agccccagcg
    36961 ccaccagaat cgcaacaatg atcaccggga tcatgttcat caggatcagg gcgaaagtga
    37021 attccaccgg ctggccgttg atctgaacac cggagtacat agctaccaga ccaccagcga
    37081 tacaaccaat cggaatggtc acaatgcccg ccagcacgcc gagcgccaga taacgacggt
    37141 cagaaggttc gataatgccg agcgccaccg gaatggaaaa cacaatcgtt ggccccatca
    37201 tcgacccgag aattaaccca gagtagagcc acgcggctac gtcgccgccc gccagctctt
    37261 tggcgaggaa gaagccgccc atatcgcacg ccagcagtgt tccggcgaac atcgatgggt
    37321 ttgcaccgag catttcgtaa accgggataa ttaccggccc gagtacgtga gccagtaccg
    37381 gtgccagcgc ggtcataccg accatcgcca ggcccagtgc gcccattgcc ataaagcctt
    37441 cttcgaactg accgcctgat ccttcgatac ttttaccgaa cttaccgagg aaacgagcag
    37501 aaccgccgaa ctgcgacagg atcctgtcta cggcagctat cagcataaag aacatcatga
    37561 tgtacatgat gatttcgtta attcccatcg cctttactcc ctgttagttg ttatttatta
    37621 gcggatgcgg cgtgaaaacc ttatccgccc tacatgtgca atcccgaagg ccggatcaga
    37681 cgcggcaagc gtcgcatccg gcaattgcac cgcgccactg gcggatgcgg cgtgaacgcc
    37741 ttatccgccc tacatgtgca atcccgtagg ccggataaga cgcggcaagc atcgcatccg
    37801 gcaattgcac cgcgccactg gcggatgcgt cgtgaacgcc ttatccgccc tacatgtgca
    37861 atcccgtagg ctggataaga cgcggcaagc gtcgcatccg gcatttattg cgccgctgcg
    37921 tacaggccga caatctgctc caggctggcg gtacgcgggt tgctgcgcag acaaatatct
    37981 tcctgcgcgg cctgcgccca tgcaccgtaa tgcgcagatg tcgcgccaac atcgcccagt
    38041 cgtttaccaa tcccaacttc cgcaatcagc tcacttaccg cgttaatagc gtcacgatcg
    38101 tcggattttt tagttcgcag tgcccgacca atctgactaa agcgttcacg acaaaccatc
    38161 cggttaaact ccatcaccgt tggcagcaac atagcgttcg cgagaccgtg tggaatatgc
    38221 agcgccgcgc ccggctgatg cgccatcgcg tggcacaacc caagacccgc actggaaaac
    38281 gccattcccg acatacatga agccagcaac atgctctcgc gcgcggcaag gtcgtgaccg
    38341 tagcccaccg ctttcggcag cgatttgcca atcatcgcaa tggcaccaat cgccagactg
    38401 tcggtaaacg gtgtagcgtt cagggcgctg tatgcttcaa tggcatgggt taacgcatca
    38461 atgccggtca tcgccgtgac atgcgacggc acaccttcgg tcaatgcggc gtcgaggatc
    38521 gccacatccg gcatcagcga ggcatgggct aacacctgct tgcgcccgct caccgcgtcg
    38581 ataatcaccg ttacattggt ggtttcagag ccggttccgg cggtagttgg aatggcaatc
    38641 agcggcaagc gcggttgcag aacgctggtt tctgacatct ctgccagcgt gctatccggg
    38701 ttcgtcacca gcaacgccac ggcttttgcc gcatccagca ccgagccgcc gccaaatgcg
    38761 atcaccccgt cacaacctga ctcacgcaac tgcgccacgg ctgcacacac gtcggtaatg
    38821 cacggttcgc ccaccggaca tggccagagc gtcatggcga tacctttaac ggccaggcta
    38881 cgcgtcagcc cggcggtcat ccctgcctga tgcaaaaagc tgtctgccat cacgaacaga
    38941 tgtttcagcc cacgcgtttg cgcttgctgt ccgcaactgc tcaccgagcc cggaccgcaa
    39001 agcgtcaccg gtggaacgct aaatgttttt acccgttgca gattcagggt atcgaacgcc
    39061 tgaaagagcg cggtctgcaa ttcattttgc atatagcccc tccgctttct ctcgcccact
    39121 actggcgatc gccagcgggg tcataaacaa actgtgctgc ggtaaatgca cctgtaacac
    39181 cgggaattgt ttgcgaaaca gttccgccac gcccggttgc atacaagagc cgcccgccag
    39241 ccataaatca gtaatgccct gcccttcaat atgccgggcg acaatgtccg ccattttttc
    39301 gtacaccggt ttcaccgtag gccaaatctc ttcaccgtga ccgcgcttgt actgctccgc
    39361 ctcttccagc gaaatacggc gatttccggc gagagtcaga gagatgtgat gtccgccggt
    39421 tgcttcatcc gccgagtacg tcactttgcc ctttttcacg atggcaatgc cggtagtgcc
    39481 gccgccgata tccactacac cggcgttgtc cagttgcagc agatccgcaa ctgcggttgg
    39541 ctcatccagc acatggctca cttccagtcc ggcagattcc agcacgttga tggaaatacg
    39601 cgggtccgta cctggaggaa atgaagtcgc cgcatggcta aaacgacgac cgaattgctg
    39661 ctcgagcgtg tcgagatggc gacgaacaat ggtgaccgcg ccgaagaaat cccagacgat
    39721 gccgtcgcga accacatcgg cccagtcgag gcataccgcc actggctgat cctcgcggtc
    39781 gacaaccatc gacaccacat cgcaggtgcc cagatccacc cccagccaca gcggagattc
    39841 cgtcgcggcg ggcgtctggt tacacagcgt tgccgccgtt tgcagacgtg gggtgagcca
    39901 ttgttcgtcg tgcgccatct gttactcctt aaacaatgcg aaacgcatcg actaatacac
    39961 agcgacgcag acggacaaac gtacgcgcgc tggttacccc ttcaccggtt ggcgtggtga
    40021 tggtcatggt ggtccagcct tccccgccca gccccagccc ggcaatgcac ggtccgttct
    40081 taacgaaaat gctggtatca atagcattcg ccatctggtt catgttttcg atgttgcgcg
    40141 agtgcattgc cgccgtgtgg tggcaaccgc cctccagttt caccgctagc gcaatggcat
    40201 ccgccacgtt ggcgacgcgc acgacgggca acaccggcat catcagttca gtcacggcaa
    40261 acggatgttc tgcggtggtt tccacaaaca gcaggcgcgt ttcttgcgga actttaaggc
    40321 cgattgccgc cgcgattttg cctgcgtcgc gaccaaccca gtcacggctg acggtgcctt
    40381 ttccgcgctc gtcgatattt ttcagcaaca ccggttgcag ctgctgcgcc tgttctgcgg
    40441 tcagtttcac cgcgtgctgg ccttccatca gacgcatcag ttcatcggct acgctatcaa
    40501 caacaatcag taccttttcg tcggcacaaa tgatgttgtt atcgaaagac gcgcctttga
    40561 cgatggactg agcagcacgg gcgaggtcag cggtttcatc caccactacc ggcgggttgc
    40621 cagcgcctgc ggcaatcaga cgtttattgg tgtgtttacg cgccgcttct actaccgctt
    40681 cgccgccggt caccaccagt aggccgatac ccggaaactt gaacaagcgt tgcgcggttt
    40741 cgatatccgg atttgccaca gtaaccagta agttttccgg cccgcctgcg gcaacaatcg
    40801 cctggttgag cagcgtgatc gcccgctggg agactttttt cgccgccgga tgcggggcaa
    40861 aaatgacgct gttgcccgcg gcaatcaggc tgatggcgtt gttaattacg gttgccgccg
    40921 ggttagtgga aggcgtcacc gaagccacca cgccccaggg tgcgttttca attagggtca
    40981 ggccgttgtc gccagtcagc acttgcggag agaggcactc aacgcctggt gtgccgcgcg
    41041 cctgagcgac gttttttgca aatttatctt caacgcgccc catgccggtt tcactgacgg
    41101 caagttccgc taaatctctg gcgtgttttt cgcctgcttc acgaatggca gcaatggcta
    41161 actggcgcat tgccacgctc tttaaccctt gctgggcaat tttggctgcc gcaacagcgt
    41221 catccaggga cgcgaaaacg cccatctcat gaacggcggc gggcggcgtg tcactgcttt
    41281 gcattttcag cagtaccgct ttcaccacct gttcaatatc ctgttgattc atgatgttct
    41341 gccttatttg tggaaaatta cctgaccgcc agacaccacc tcatcgacga tgccaatcac
    41401 gcacagatcg accggtgacg tttcgctttt atgcgcctgg cgggcggaac tgccactcac
    41461 cagcaacacc cactccccgg ttcccgcgcc aatattgtcg atggcgacgg cacattgccc
    41521 gtcaggatta ccttgtggat caatcatttc caccatcagc aatttgtcat gcgccagtcc
    41581 gtgatggcgt acggtacaaa caatttgtcc agtgacgact gccagtttca tacccgcctc
    41641 cgtggcgtat ttcaggtaaa agctccccct accctccgcg gaaggtaaaa tgaaaaatga
    41701 gagagcgtga cgcccgaatc gacgtcacac agggtgatta caggttgctg ctatcgcctt
    41761 tcaggccgat cgggaacact tcttccaggt cgccgtgcgg acgcgggata acgtgtacag
    41821 ataccagctc gccgatacgc tgtgccgctg cggcaccggc atcggtcgcc gctttacagg
    41881 ctgcaacatc accacgaacc atggcagtac acaggccgcc gccaatctgc ttaacaccaa
    41941 ccagcttgac gcgcgcagct ttcaccatcg cgtcagaagc ctcaatcagt gcaaccaggc
    42001 cccgggtttc gatcattcct aatgcttcca ttgtgctttc ctctttatca gggtccagaa
    42061 cgggaccgtt cattcaacca gtgtttgtaa actgctttcg cggttcactt ctgtctgacg
    42121 cggcacagct gccaccagcg ccagctcgat aatttcctgc acactacaac cacgagagag
    42181 atcgtgcatc ggcgcggcaa gtccttgtat caatggcccg acggcacgat atccgccgag
    42241 tcgttgtgcg attttgtagc caatatttcc ggcttccagc gacggaaaaa ccatcacatt
    42301 ggccttgccc tgtagcgggc tggcaggcgc tttttgcgcc gccacttccg gcacgaaggc
    42361 ggcgtcaaac tgcaactcgc catcgactac cagctttggt gcgcgctcac ggacgatttc
    42421 tgtcgcctgc tggacattgg caacgcaggg gtgacgggcg ctaccgttac tggaaaacga
    42481 cagcatcgcc acgcgcggct cttctccggt gatggcgcgc caggtttcgg cgctggcaag
    42541 cgcgatatcc gccagctgcg ccgccgtcgg ctgcggtacc acgctgcaat cggcaaagcc
    42601 caacgccgga ccgctgtact gtggcagcat caggaaaatg gatgagagcg ttttacagcc
    42661 tggctgcaag ccgataatgc gtaatccggc acgcagcaca ttcgccgtgg aagagaggtt
    42721 gcccgcgata cagacatccg ctttaccggc gctgaccatt gcggcggcga acatcagcgg
    42781 atcggtaagt ttttccagcg catccggcgg cgtttttccg cccgcgcggg ccagccagcg
    42841 atgagcaaat tcttcccgca ttgcgaggtt gccatgcgga tcaataacct gtagcccgtc
    42901 catcgctacg ccatgactga gcgcaaactg acgaagttca aacggattgg cgaccagaat
    42961 gggcgttgcc agaccttgtt gatgtaaata ttgcgcagct ttcagcacac gttgatctaa
    43021 cgcatccgga aaaaccactc tggcgggcgc tcgcaacgcc agttcacgac aacgttcaat
    43081 aatcattgcg tttcccccag tcgttgttgg atctgcttca ccgttagcgg cggcttcgtt
    43141 acgctcagga tcatcatcac gtagaccgtg ctcgaaaggc ggttcagcgc ctgcaagatg
    43201 tccgggcgta atacttcaaa gctgcgggtt ataaatacct gcgccgctac ggtttcggtt
    43261 tcccgcactt tggtgcgtag cagattgagc aacgcagcat cgcgcccgtg gctggcttcc
    43321 ggcaccagat gatcgtggtc gagatagcgc agcggctggt gagaaagccg atgcagatct
    43381 tcgtcagaaa gcccgacaat cgcttgatcg cccagcggtt cccccagcgc atcggcacgc
    43441 ataatgttgc caagacgcga acggatatcc gccagccacg gctgccacgg ttccgccagt
    43501 tcaatttgca gccacacagc cagcgcaatg gtgctgtcga gaaccgcgcg aaaacccaga
    43561 cgcggatcgc ttttggcaac cattttttcc gccgacagat gagtcagcgt atccggtttt
    43621 tttgccaccg gctggcggca cagttcgcag cacgcctgcg gatgttcatc gctactggtc
    43681 agcccgtgaa cgggctgtgg ctgctgctgc tcatcgtcaa caaacaggcg gccctgctcg
    43741 tcaataaact tgatgcgcag atggcggctt tccagcaact cccgggcaga gggcgtcagg
    43801 cgactgtccg cagggagatg gatctctgcg ccttcgctga gcgtatggtt cgctcttagc
    43861 catgcttcgg tgatgaaatc tttcataggg attgccagtt agccggccag gcgacataca
    43921 ggaatttcac gctggatgtc gtaccaaatt cgatgctgga gcctttcggg ataaacatta
    43981 cgtcgcctgc tttggcgatc atggtttcgc cttcgtggcg aacatgcagc tccccttcca
    44041 gcaccatgtc gatttcgtcg tagttcaggg tccacgggaa gaatgcgttc tcccactgca
    44101 taaaaccagc ggccatgctg ctgccgtcgt ctccggtcac aaggtcagtt aagcccacgc
    44161 aatgcggctc tgcgccgtcg aaacgaccaa acttgacgct gctgccatca atcactttga
    44221 tgccgccttt gccggtcacc gatttgaagc tcggctgcat tgcaccctgc tccagcgact
    44281 gtttttcctt catcactttt tccatcaact gcgcgaccag gctttcggta aactggcctt
    44341 ccggcagctg ggcaatgatg gtttcgcgga tgcgctgact ttcggtttta tcggctggca
    44401 cgctggcggg cacggacgcc gttaccggga tcgattcatc acattcggtg atggtaaaac
    44461 ccagtagatc cgccacttca cgggcttccg gggtaataat gctggcgcgc agaaccacgg
    44521 acattgcctg ttcgccgcgc gcgtgtgctt cacgaatatc attcgctgtg atgagttttt
    44581 tcacctgctt cctcctgttt ggtgagagat gccagataat ccaccagttg ctgcacgctt
    44641 tgcgggtcgt ggctgtttag ctcgaaaatt ggctcttcga agccagtctc aagcaataac
    44701 tttcgcgttg cggcgacgtc agcatccggc atgtccgttt tactgatgac ggcgatttgc
    44761 cgtttgctga cgccaatatc cagcaacccg gcaggcaacc gactttccgg gtcattcgcg
    44821 ccgtggacat agatcagcat gtccacatcc tgtagcgtgg taattaaggc gtgataccag
    44881 cggggatggc taaaatattc acccggcgta tcaatatcgc ccttatcatt aaattccacg
    44941 gcctgtgttt ttctggcgag ggtataattc ccctgcaacg cattaaataa cgttgttttt
    45001 cctgccccga cggtacccac aaacgcaatt cgtttcatga atgcctcgta ttaacttttg
    45061 gtcatttcac aaagcgtata atttaataag cgacctaaac cactgacggt ttgtgataac
    45121 gcttcttcta ctgcgcccac cgagccataa atcaccaatg caccactgaa gcggtcgaga
    45181 aaaccgatat gcacatcagc ggctttcaat gctaaatcac cggcgatcat cgccgtttcg
    45241 ccaggagtta gcgtcataat gccaattgcg cccgcatccg gaacgccgat tttcttcgcc
    45301 agttcctcgc cgggatgcgc gatgagatgc gccagcgtga cctgtttgcc aggcacaaat
    45361 tcctgaatga tgcgttcttt atccatgagt cgccgcctgt gaaaaacttt cgtgtatcgt
    45421 gacaattttt cgtcagagta aataacaaaa ttcggcaagc gcgtttaaaa ggtgaggtag
    45481 atcactaaga atggagagat attcgctgtg gtgcataaac tttcatatgc aacgcatgaa
    45541 tataaataaa ataatatcga gatattatat agactgcttt ttttattatt cgcaaactca
    45601 gtaataagct ttactaatat attgcagctg gaccgcttac cctgaatatt cagggtaagc
    45661 gtgagagtta aaaaagaatt acagcggttg ggtttgcgct tcaaccacgg ccagcgccac
    45721 catgttgacg atacgacgta ccgatgcgat cggcgttaac acgtgaaccg gtttcgccac
    45781 gcccatcagc accggaccga cagtcacacc ttccgagctg gaaacgcgca gtaagttgta
    45841 actaatgcgg gcagcttcca tgtttggcat taccagaata ttggcggaac ctttcaaagg
    45901 gctgtccggc atacggtcgt tgcgaatcgc ttccaccagc gctgcatcgc cgtgcatttc
    45961 accatcaatc atcagttctg gcgcacgttc cctgaccagt tccagcgcct gacgcatttt
    46021 gctcgacgac gggcagtcag aagaaccaaa gttggagtgc gacaacagag caacgcgcgg
    46081 ttcaataccg aaacgacgga cagtttctgc cgccatcaag gtgatctccg ccagctcttc
    46141 tgcgtctggt tcgtcattga cgtaggtatc ggcaataaag gtgttaccac tcggcagcag
    46201 cagcgcgttc atggcacctg cggtgtgaac gccatcgcga taaccaaaga catttttcac
    46261 cacgctaaaa tgttcatgat aatcacccac cgtaccgcaa atcattgcat cggcttcgcc
    46321 acgctgaacc atgatcgcgc cgatcactgt cgggttaccg atcagcgcac gctgcgcctg
    46381 ttcctgagtg acgccgcgac gcttcatgat ctggaagtat tcggtccagt actctttaaa
    46441 gcgcggatcg gattcgttat tgacgatctc aaaatcaacg cccgctttga tctgcaagcc
    46501 cagtttctga atgcgcattt cgatcacgtt cggacgaccg ataaggatcg gtttcgccag
    46561 tcccagcgtt accagttcct gagtggcatg caaaacgcgc gcctcttccc cttccggcag
    46621 aactacgcgc ttcggcgctt tgcgagcctg ggagaaaatc ggcttcataa acaggttggt
    46681 tttgtaaacg aactcagtca gcttgtcgat gtagacgtcg aaatcagcaa tcggacgcgt
    46741 cgccacgccc gactccatcg ccgctttcgc gaccgcagga gcaatcttga ctatcagacg
    46801 cggatcaaac ggttttggaa tgatgtattc agggccaaag ctaagatcct gatcgccata
    46861 cgcagaagcc accacttcac tctgttccgc atgggccagt tctgcaatcg cacgtaccgc
    46921 cgccagtttc atctcttcgt tgatggcggt tgcgccaacg tccagcgcgc cacggaagat
    46981 aaacgggaag cacaggacgt tgttcacctg gttcgggtag tcggaacggc cggtacagat
    47041 gatggcatcc ggacgcactt ctttcgccag cggcggcaga atttccggtt ccgggttcgc
    47101 cagcgccagg atcattggcg ctcgagccat tttcttcacc atttcctggg tcagcacttt
    47161 cgggccggaa cagccgagga aaatatccgc gccttcaatc acatcatcga gggttcgttt
    47221 gccgtcatcc accaccgcat atgcggcttt ggtttccgcc atgtttggct cacggccctg
    47281 atagataacg ccttttgaat cgcaaaccac gatgttatgt ttttgcagcc ccagagccac
    47341 cagcaggttc atacaggcga ttgctgcggc acccgcgccg gaaaccacca tccgcacgtc
    47401 agagatgttt ttctccacca cgcgcaagcc gttgaggatg gcggctgtgc tgataattgc
    47461 cgtgccgtgc tgatcgtcgt ggaataccgg aatattcatc cgctcgcgca gtttctgttc
    47521 aatatagaaa cattctggcg ctttaatgtc ttcgaggttg atgccgccga aggtcggttc
    47581 gagcgcagcg acaacttcaa taaatttgtc cgggtcgagt tcatcaactt caatgtcaaa
    47641 tacatcaatc ccggcgaatt tcttaaacag aacgcccttg ccttccatca ccggtttgcc
    47701 cgccagcgcg ccaatgttgc ctaaccccag caccgccgta ccgttagaga tcaccgccac
    47761 caggttaccg cgcgcggtat atttgtaggc ttttaacggg tctttttcga tttcaagaca
    47821 aggtgcggca acgcctggtg agtaggccag cgccagatcg cgctgcgttg ccagaggctt
    47881 ggttggagaa acctggattt tccctggaac tggaaattca tggaaatcaa gtgcactttg
    47941 ttttaactgg tcatccattt ggttgttcct ttcacgtaac gttcacaaat aaagtgtgtg
    48001 ggcaacaccc cctgcccaca acgtggcgca cattattacc ctgccggagt ctacagactt
    48061 tgagcaagtc caaactctca ccattaatat aatgttttgg taataatcct ataacactga
    48121 tgttacctgc ttaatccagc aataccatgc ctgtctgcta tgcttttttg atgcgtttag
    48181 cgaaatttct cagaagtgtg aattaacgca ctcatctaac actttacttt tcaaggagta
    48241 tttcctatga acgagttaga cggcatcaaa cagttcacca ctgtcgtggc agacagcggc
    48301 gatattgagt ccattcgcca ttatcatccc caggatgcca ccaccaatcc ttcgctgttg
    48361 ctcaaggctg ccggattatc acaatatgag catttaatag acgatgctat cgcctggggt
    48421 aaaaaaaatg gcaagaccca ggaacaacag gtggtcgcag cgtgtgacaa actggcggtc
    48481 aatttcggtg ctgaaattct caaaatcgta cccggtcgcg tgtcaacaga agttgatgca
    48541 cgcctctctt ttgataaaga aaagagtatt gagaaggcgc gccatctggt ggacttgtat
    48601 cagcaacaag gcgttgagaa atcacgcatt ctgatcaaac tggcttcgac ctgggaagga
    48661 attcgcgcgg cagaagagct ggaaaaagaa ggtattaact gcaacctgac gctgctgttt
    48721 tcttttgcac aggcgcgggc ctgtgcagaa gcgggcgttt ttctgatttc gccgtttgtc
    48781 gggcgtattt atgactggta tcaggcacgc aagccgatgg acccgtatgt ggtggaagaa
    48841 gatccaggcg ttaaatcagt acgcaatatc tacgactact ataagcaaca ccactatgaa
    48901 accattgtga tgggcgcgag cttccgtcgc accgaacaaa tcctcgcctt aaccggctgc
    48961 gatcgactga ctatcgcacc gaatttgctg aaggagctgc aggaaaaagt ttcgccagtg
    49021 gtacgtaaat taatcccacc ttctcagacg ttcccacgcc cagctcccat gagcgaagcg
    49081 gagttccgtt gggagcacaa tcaggatgcg atggcggtag aaaaactgtc tgaaggcatt
    49141 cgtctgttcg ccgttgatca acgcaaactg gaagatcttc ttgccgccaa actataaacc
    49201 agccacggag tgttatatgt cccgaaaaga ccttgccaat gcgattcgcg cactcagtat
    49261 ggatgcggta caaaaagcca attctggtca tcccggcgcg ccgatgggca tggctgatat
    49321 tgccgaagtg ctgtggaacg attttcttaa acataaccct accgacccaa cctggtatga
    49381 tcgcgaccgc tttattcttt ccaacggtca cgcgtcgatg ctgctctaca gtttgctgca
    49441 tctgaccggt tacgacctgc cgctggaaga gctgaagaac tttcgtcaac tgcattcgaa
    49501 aacccctggc cacccggaaa tcggctatac gcccggagtt gaaaccacca ccggtcctct
    49561 tggacaaggt ttggcgaacg ccgtcgggct ggcgatagcg gaacgtacgc tggctgcaca
    49621 gtttaaccag ccagaccatg agatcgtcga tcacttcacc tatgtgttta tgggcgacgg
    49681 ctgcctgatg gaaggtattt cccacgaagt ctgttcgctg gcgggcacgc tgggactggg
    49741 caagctgatt ggtttttacg atcacaacgg tatttcgatt gatggagaaa ccgaaggctg
    49801 gtttaccgac gatacggcaa aacgttttga agcctatcac tggcatgtga tccatgaaat
    49861 cgacggtcac gatccgcagg cggtgaagga agcgatcctt gaagcgcaaa gcgtgaaaga
    49921 taagccgtcg ctgattatct gccgtacggt gattggtttt ggttcgccga ataaagcagg
    49981 taaggaagag gcgcacggcg caccgctggg ggaagaagaa gtggcgctgg cacggcaaaa
    50041 actgggctgg caccatccgc catttgagat ccctaaagag atttatcacg cctgggatgc
    50101 ccgcgaaaaa ggcgaaaaag cgcagcagag ctggaatgag aagtttgccg cctataaaaa
    50161 ggctcatccg caactggcag aagagtttac ccgtcggatg agcggtggtt taccgaagga
    50221 ctgggagaaa acgactcaga aatatatcaa tgagttacag gcaaatccgg cgaaaatcgc
    50281 tacccgtaag gcttcgcaaa atacgcttaa cgcttacggg ccgatgctac cggagctgct
    50341 cggcggttcg gcggatctgg ctcccagcaa cctgaccatc tggaaaggtt ctgtttcgct
    50401 gaaggaagat ccggcgggca actacattca ctacggggtg cgtgaatttg gcatgaccgc
    50461 tatcgccaac ggcattgcgc accacggcgg ctttgtgccg tataccgcaa cgttcctgat
    50521 gtttgttgaa tacgcccgta acgccgcgcg gatggcggca ctgatgaaag cgcggcagat
    50581 tatggtttat acccacgact caattggtct gggcgaagat ggtccgacgc accaggctgt
    50641 tgagcaactg gccagcctgc gcttaacgcc aaatttcagc acctggcgac cgtgcgatca
    50701 ggtggaagcg gcggtgggct ggaagctggc ggttgagcgc cacaacggac cgacggcact
    50761 gattctctcc aggcagaatc tggcccaggt ggaacgtacg ccggatcagg ttaaagagat
    50821 tgctcgtggc ggttatgtgc tgaaagacag cggcggtaag ccagatatta ttttgattgc
    50881 caccggttca gagatggaaa tcaccctgca agcggcggag aaattagcgg gagaaggtcg
    50941 caatgttcgc gtggtttccc tgccctcgac cgatattttc gacgcccagg atgaggaata
    51001 tcgggagtcg gtgttgcctt ctaacgttgc ggctcgcgtg gcggtggaag caggtattgc
    51061 cgattactgg tacaagtatg ttggtctgaa aggggcaatt gtcgggatga cgggttatgg
    51121 ggaatctgct ccgacggata agctgttccc gttctttggc tttaccgccg agaatattgt
    51181 ggcaaaagcg cataaggtgc tgggagtgaa aggagcctga tggtgatcgc cggatgctga
    51241 ttgccggacg ctgattgccg gatgcgacgc tgacgcgtct tatccggcct acatgtccct
    51301 acatgtcccg ccattttgtt taacgggtga tccacaacgt gggccaggcg tctggcccat
    51361 gccagttatc gcaggtgggt tctgcggcat aacgcaccag gcgaaaacgc tgaccgtcaa
    51421 agcgccagcg cgcctgaatg ccacaatcgc ttaatccgcg ccctttcgct aaggtcacca
    51481 gttcacgcga tttctcatca aatgttgcgt tcatcagttc cagttcattc gtctcctgac
    51541 cgttgttgaa cggcaaacgc aaccgaaccg ggcgcgaggc tagtggcttt ttacgcgaca
    51601 caatccatgc caaatcaatg gtgttatagg cccctgcctc acagctaatc atcatcagcg
    51661 ctttatcatc agtcagcgca gtgacattca cctcacgacg taacggatca agcgagcagc
    51721 gcagaccatt catccgccag tttccataat ccagcaaatc gttacgttct tcgagtgaga
    51781 gtggcgtcgg cgttgggtta accaccgcga cctctttcaa cgcaggcgct ggcggtacgc
    51841 tgagcggcgg ttcgtcccct ttctttatcc acgcggtttc actgccaacg cgcttttgct
    51901 gagcatcaat aaacaacaac gccgctttta agccactcag agaaatggtc tgattgccgt
    51961 cgcgtaaggt gatcgccttc ccttcctgaa tcatctgcaa aaacgcggtg atggttgccg
    52021 tatcatcggt tactaataac catggtgaaa tccgccactt gtcaccactt aacgccaacg
    52081 gctcaccatc taacagcagc cgtggcgcta tctccccttc agacgcatcc ggcgatttca
    52141 acccgccgcg ctcaatacgt aaaacggcat cggtatgcgc cccggcgctg cggctcaggg
    52201 tcatcaccag tccattatga tcgcccgtat tgcgcgccac gcagaagttt tggttattac
    52261 aggtgacctg ccagtcggaa aacgcccgtt gcgctggtgc cgcccacacc aaagacgtcg
    52321 gtaacaaagc gaaaaaaaag agaaggaaaa tgcgatagcg catggacggt acgaccccag
    52381 aagttcaaac aaacagcaca agtcgtatct tcgttgcgca ggtagcgtgg ctcaatcgga
    52441 tttgtcggat tgagccaata ataaacaacg aaatattatt gttatttttc agtccattaa
    52501 atgtgacgtt tgcaaatagt taagcaataa caccgtctta ccgtcacgta tctcgccggt
    52561 tttgatcatc tccagcgcct ggctgaacgg cagctcgagc acttcaatat cttcatcttc
    52621 gacaccgcca ccggcgttag cgcgctgatt gtcactgtat tcggcgataa aaaagtggat
    52681 tagctcagtc acaccgcctg gcgacatata cagttcaaat aatttgcgca cttcgcccac
    52741 ttcatagccc gtctcttcaa tcgcttcttt acgaatgcac acttccggtt cgtcgttatc
    52801 cagcagcccg gcgcaggttt caatcagctg cccgctttca ttgccattaa cccaggtagc
    52861 gacacggaac tgacgaatca gaaccacggt ctttttcttc gcgttgtaca ggaggatcgt
    52921 cgcaccatta ccgcgatcgt aaacttcacg tttatggcgg ataacttcgc catctttgcg
    52981 cgtgagatcg taagtaatgt tgtgcagggt gaaatagtta tcggagagaa ttttgtcttt
    53041 aatgagggtg atttgttgcg tcataccgac tccacagcgc gaaatgaaca attatcttac
    53101 gctgtgaagt cgggtttgtc tgcgcaggct atcagtgaga tttgactgat tttaccccca
    53161 gccagtcaat aatcccctgt gccgcatgac gtccttctgc cattgcggta accaccagat
    53221 ccgcaccacg cacggcgtcg ccaccggcga agattttcgg attggtggtc tggtaacggt
    53281 actggctttc cacatccgcg atgatgcggc cccatttgtc taccgttaca ccgtgcgact
    53341 ccagccacgg catcccgtgt ggattgaagc caaacgccat aatcaccgca tcggctggca
    53401 tgacaaattc actgccctca atcggtacag gtcgccgacg cccctgggca tccggctcgc
    53461 caagacgcgt acgcaggaaa cgaattccgc aaacgtgacc ttgttcattc agctcaagcg
    53521 ccaccggctg gacgttaaat tcgaagttgg ccccctcttc gcgggcgttc ttcacctctt
    53581 tcttcgagcc tggcatgtta gcttcatcac gacgataagc gcaggtgacg ttactcgcgc
    53641 cgtggcgcag cgcagtacgc acacagtcca tcgcggtgtc gccgccgccc agtaccacga
    53701 cgttaagtcc ggcggtattg ataaacggct cttccggtag ctcttcgagc cccataacct
    53761 gtttagtgtt ggcgatgagg aacggcagcg cgtcataaac gcccggcgca tcttcattgg
    53821 gtaaacccgc tttcatggaa cggtaagtgc ctacgccaac gaagaccgcg tcgtattgtt
    53881 ccaaaagcga atccaaagag acatctttac ccacttcaca attgagttcg aagtgaatcc
    53941 ccatcgcgct gaagatttcc cgacggcgtg ccagcaggga tttatccagt ttgaaagaag
    54001 gaatgccgaa agtgagcaag ccaccgattt ctggatggcg atcgtacacc gtcaccgcca
    54061 tgccattgcg ggtcagaaca tccgcacagg ccagccctgc cggacctgca ccgataatcg
    54121 ccacccgctt gtccactttg gtgacatggc ttaagtcagg acgccaacct ttcgccaacg
    54181 cctgatctga aatgtagcgt tcaatgttgc cgatagttac cgcaccgtgc tcatcgcgaa
    54241 tagtacaggc accttcacac aaacggtctt gcggacaaac gcgtccggta atttccggca
    54301 gggtgttggt caggtgagaa agctcgacgg cggcgtcgat gtttccggct ttcaccagtt
    54361 caatccactg cggaatatgg ttatgcagcg ggcaggtcca ttcacaaacg ctgtgctcgc
    54421 cgcacttaag gcagcgcgag gcttcccgtt gtgcctgatc ggcacggaat ggcagataaa
    54481 tttcatcaaa accggttttg cgcgcttcaa tcgccagttt atccggctcg ccacgcgcgg
    54541 gcgttgcctg catttgctcg actttactca ttaccggcat ttcttgtacc gcggtactgg
    54601 catgccacgg ttgatgctcc tgacgtgcgg tgcgcaagcg gcgggatttc gccatgccgg
    54661 agagtgcggc gtcagtgacc agctgcagcg cgtccgccgg gcaattctca acacaagcag
    54721 gaccattttc gcgccctgca caaaggtcgc atttatgcgc tgtggctttt actttgcccg
    54781 ccgcgacggg tgtcaggacg atttgcatcg taccaaatgg acaggccacc acgcaggatt
    54841 tacagccaat acacttttgc tgattgacct gaatgctgtc atcagcgtgg ctgattgcgc
    54901 cattagggca gctacgggcg cagggcgcat cttcacaatg gtgacaggtc actgcactac
    54961 gttgctgctg atgtttgata accgtaattc ggggatgaaa atgatgttgg ctcaggacat
    55021 gttgctcatc attgtgagcc atgacacaag cgatttcaca agcatgacaa cccagacatt
    55081 gctgactgtt ggccataata aaacgattca taacgacctt cttttttggt tgtaaaaacc
    55141 ttattcttta tatgagtgtt gttattaccc gacttacagg ggaatcggca atgttcatgt
    55201 gcccagaata agtaactatt tcgctataaa ctgtggcaga tcaaataatc ccatcactga
    55261 ctaaattgcg tttcagcgaa ctggaacatt aatgattttt tgtggagaag acgcgtgtga
    55321 ttgttaaacg acccgtctcg gccagtctgg cccgggcctt tttttacatt gtgctgctgt
    55381 cgattctttc cacgggtatc gctctgctaa ctctggcgag cagtttgcgc gacgctgagg
    55441 ctatcaatat tgccggatcg ctgcgtatgc agagttaccg cctgggctac gacttgcaaa
    55501 gtggcagtcc acaactcaat gcacatcgcc agttatttca gcaggcactg cattcaccgg
    55561 tattaaccaa cctcaacgtc tggtatgtgc cagaagcagt aaaaactcgc tatgcgcatc
    55621 tgaatgccaa ctggctggag atgaataatc ggctcagcaa gggcgatttg ccgtggtatc
    55681 aggccaatat taataattat gttaatcaga tagacctgtt cgtgctggct ttacagcact
    55741 acgctgaacg caaaatgctg ctggtggtgg cgatttccct ggctggcggt atcggtattt
    55801 tcacgctggt cttttttact ttgcgccgca tacgccatca ggtggttgcc ccgctgaatc
    55861 agctggttac cgccagtcag cgtattgaac acgggcagtt cgactcgccg ccgctggata
    55921 ccagtctgcc gaatgagctt ggtctacttg caaaaacctt taaccagatg tcgagcgagc
    55981 tgcataaatt gtaccgttcg ctggaagcgt cagtagaaga aaagacccgc gatctccacg
    56041 aggccaagcg tcgtctggag gtgttgtatc agtgttcgca ggcgctgaac accagccaga
    56101 ttgatgtgca ttgtttccgc catattttgc agattgttcg cgacaatgaa gcggctgaat
    56161 atctggagct gaacgttggg gataactggc ggattagcga agggcaacca aacccggaat
    56221 tgccgatgca gattttaccg gtgacaatgc aagagacggt ttacggtgaa ctgcactggc
    56281 aaaatagtca cgtttcctca tcagaaccgc tgcttaacag cgtttcgtcg atgctgggac
    56341 gcggtttgta ctttaatcag gcgcagaagc attttcagca gttattgttg atggaagaac
    56401 gtgcgaccat cgcccgcgaa ttgcacgact cgctggctca ggtactttct tacttacgta
    56461 tccagttgac gttactgaag cgttcgatac cggaagataa cgccaccgca caaagtatca
    56521 tggccgattt ttcccaggcg ttgaatgacg cttatcggca gttacgcgag ctgttgacta
    56581 ctttccgcct gacgctgcag caggcggatc tcccctccgc gttgagggaa atgctggata
    56641 cgttacaaaa tcaaaccagc gccaaactga ccctcgactg ccgtctgcca accctggcgc
    56701 tggatgcgca aatgcaggtg catttgttgc aaattattcg cgaagcggtg ctgaatgcga
    56761 tgaagcacgc caacgccagc gaaatcgccg tcagttgcgt caccgcgccg gacggcaatc
    56821 acacggttta tatccgtgat aacgggattg gtatcggtga accgaaagaa cccgaaggtc
    56881 attatggtct gaatatcatg cgcgaacgcg cggaacggct aggtgggacg ctgacttttt
    56941 cacaaccttc cggcggcggc acgttagtga gtattagctt tcgctctgcg gagggtgagg
    57001 aaagtcagtt gatgtaatgc ctcctactga ccaaagaata cttgcagtta aggttcagta
    57061 taaaagggca tgataattta cattaactcc tttttttctc cacgattggc tcgtaccttg
    57121 ccgctacagt gaagcaagtc aagcctacaa cgatacgcag aaacacgagg tcctctttta
    57181 atggcgaatt tctttattga tcgccccatt tttgcctggg tgctggcaat cctgttgtgt
    57241 ctgacaggta ccctggcgat tttttcattg cccgttgaac aataccccga tctcgcgcca
    57301 ccgaatgtgc gagtgaccgc taactatccc ggcgcatcgg cccagacgct ggaaaacacc
    57361 gtgacccagg ttatcgagca aaatatgacc ggcctcgata atctcatgta tatgtcatct
    57421 cagagcagtg gcaccggtca ggcatctgtc actttaagtt ttaaagcagg caccgatccg
    57481 gacgaagccg tgcagcaagt acaaaaccag ctgcaatcag ccatgcgaaa gttaccgcag
    57541 gcggtgcaaa atcagggcgt gacggtgcgt aaaaccggcg ataccaacat tctgaccatt
    57601 gccttcgtct ctaccgatgg ttcgatggat aaacaggata ttgctgatta tgttgccagt
    57661 aatattcagg acccgttaag ccgcgtaaat ggcgtcgggg atatcgatgc ctatggttcg
    57721 caatattcca tgcgtatctg gctggacccg gcgaaactca acagtttcca gatgacggct
    57781 aaagatgtca ctgatgccat tgagtcacag aacgcgcaga ttgcggttgg gcaacttggt
    57841 ggtacacctt ccgtcgataa gcaggcgctc aacgccacca ttaacgccca gtcactgctg
    57901 caaacaccag aacagttccg cgatatcacc ttgcgggtga atcaggacgg ctcagaggta
    57961 aggctgggcg atgtcgccac cgtcgaaatg ggggcggaga aatacgatta tcttagccgc
    58021 ttcaatggta agccagcctc cgggctgggg gtaaaactgg cctccggcgc taacgaaatg
    58081 gcgacagcgg agctggtgct caatcgtctc gacgagctgg cgcagtattt cccgcatgga
    58141 ctggaataca aggtggcgta tgaaaccacc tcgtttgtta aagcctccat tgaagacgtg
    58201 gtgaaaacgc tgctggaagc tatcgccctg gttttcctcg ttatgtatct gttcctgcaa
    58261 aacttccgcg ccacgctgat acccactatc gccgtgccgg tggtgttgat gggaaccttc
    58321 tccgtacttt acgccttcgg ttacagcgtc aacaccttaa ccatgttcgc gatggtgctg
    58381 gcgatcggtc tgctggtgga tgatgccatc gtggtggtgg aaaacgtcga acgtattatg
    58441 agtgaggaag gactcactcc tcgcgaagcc acacgtaaat cgatggggca gatccagggg
    58501 gcactggtcg ggattgcgat ggtgctttcg gcggtatttg taccaatggc cttcttcggc
    58561 ggcaccaccg gtgccatcta tcgccagttc tctattacca ttgttgcggc gatggtgctg
    58621 tcagtactgg tagcgatgat cctcactccg gctctgtgtg ccacgctact taagccactg
    58681 aaaaaaggtg agcatcacgg gcaaaaaggc ttttttgcct ggtttaacca gatgtttaac
    58741 cgcaacgccg aacgctacga aaaaggggtg gcgaaaattc tccaccgtag cctgcgctgg
    58801 attgtgattt atgtcctgct gcttggcggc atggtgttcc tgttcctgcg tttgccgacg
    58861 tcgttcttac cgctggaaga ccggggcatg tttactacct cggtacaatt gcccagcggt
    58921 tcaacccaac aacagaccct gaaagtcgtt gagcaaatcg agaaatatta cttcacccat
    58981 gaaaaagaca acatcatgtc ggtgtttgcc accgttggct ctggccctgg gggcaacggg
    59041 caaaacgtgg cgcgaatgtt tatccgcctg aaagactgga gcgaacgcga cagtaagacc
    59101 ggcacctcat ttgccattat cgagcgtgca acgaaggcgt ttaacaagat taaagaagct
    59161 cgcgttatcg ccagcagccc gccagcaatt agcggtcttg gtagttctgc gggttttgat
    59221 atggagttgc aggaccacgc tggagcaggt cacgatgcgc tgatggcagc acgtaaccag
    59281 ttgctggcgc tggcggcgga aaacccggag ctaacccgtg tgcgccataa cggcctcgac
    59341 gacagtccgc agttgcagat tgatatcgac cagcgtaaag ctcaggcgct gggcgttgct
    59401 atcgacgata ttaacgacac actgcaaacc gcctggggtt cgagctatgt gaatgacttt
    59461 atggatcgcg gtcgcgtgaa gaaagtctat gtgcaggcag ctgcgccgta tcgcatgctg
    59521 ccagatgaca tcaatctctg gtatgtccga aataaagatg gcggcatggt gcccttctct
    59581 gctttcgcga cctcacgctg ggagacaggc tcgccgcgtc tggaacgcta taacggttat
    59641 tctgcggttg agattgttgg ggaagccgca ccgggggtca gtaccggtac ggcgatggat
    59701 attatggaat cgttagtgaa gcagttgcca aacggctttg gtctggagtg gacggcgatg
    59761 tcgtatcagg agcggctttc cggcgcgcag gctccggcgc tgtacgccat ttccttgctg
    59821 gtggtattcc tgtgtctggc tgcattgtat gaaagctggt cggtaccgtt ctcggtaatg
    59881 ctggtcgtgc cgctgggggt aatcggcgcg ctgctggcaa cctggatgcg cgggctggaa
    59941 aacgacgttt acttccaggt gggcctgtta acggtcattg gtttatcggc gaaaaacgct
    60001 atcctgatcg tcgagtttgc taacgagatg aaccaaaaag gccacgacct gtttgaagcg
    60061 acgctccacg cctgccgtca gcgtttacgc ccgattctga tgacctcgct ggcatttatc
    60121 ttcggcgtat tgccaatggc aaccagcacg ggtgccggtt ccggtggtca gcatgcggtg
    60181 ggtactggcg taatgggcgg gatgatttca gccactattc tggctattta cttcgtgccg
    60241 ctgttctttg tgctggtgcg ccgccgcttc ccgctaaagc cgcgcccgga ataagcaata
    60301 aaaaaggcga catgccaatg tgtcgccttt ttcaactttc cgattaagaa cctgctcagc
    60361 gggttcttgc tgtttgtact ttgtctcagg aattacttac gaagcataac ttcgataaag
    60421 tctttccagt tccccagttc acgttcaatc ataacaacct ctcttataat tatgggtatt
    60481 ctacggaaac aatataccgt ggtgaagcta atttactcga ttgctgcgat gactacctcc
    60541 gggggacaaa ccttatgtaa atactatggt cctgcggtga taatttgtat gtgatacaca
    60601 gcaacatttc gagatattca tacggcatct aatactgatt taattctggt taaaatacag
    60661 acagataaca agatgaat
//
LOCUS       DAPGOF010000005        55757 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.005, whole genome shotgun sequence.
ACCESSION   DAPGOF010000005 DAPGOF010000000
VERSION     DAPGOF010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 55757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.005"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            433..948
                     /locus_tag="RHV13_000246"
     CDS             433..948
                     /locus_tag="RHV13_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDV1239813.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            1166..1894
                     /gene="artP"
                     /locus_tag="RHV13_000247"
     CDS             1166..1894
                     /gene="artP"
                     /locus_tag="RHV13_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HDV1239814.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            1912..2643
                     /gene="artJ"
                     /locus_tag="RHV13_000248"
     CDS             1912..2643
                     /gene="artJ"
                     /locus_tag="RHV13_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV1239815.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KFNAALEKVKKDGTYETIYNKWFQK"
     gene            2650..3366
                     /gene="artQ"
                     /locus_tag="RHV13_000249"
     CDS             2650..3366
                     /gene="artQ"
                     /locus_tag="RHV13_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HDV1239816.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLADGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            3366..4034
                     /gene="artM"
                     /locus_tag="RHV13_000250"
     CDS             3366..4034
                     /gene="artM"
                     /locus_tag="RHV13_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HDV1239817.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLIVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            4325..5056
                     /gene="artJ"
                     /locus_tag="RHV13_000251"
     CDS             4325..5056
                     /gene="artJ"
                     /locus_tag="RHV13_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HDV1239818.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(5231..6358)
                     /gene="rlmC"
                     /locus_tag="RHV13_000252"
     CDS             complement(5231..6358)
                     /gene="rlmC"
                     /locus_tag="RHV13_000252"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HDV1239819.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPQQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEHQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVTHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(6399..6887)
                     /locus_tag="RHV13_000253"
     CDS             complement(6399..6887)
                     /locus_tag="RHV13_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HDV1239820.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQV
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(6947..7792)
                     /gene="potI"
                     /locus_tag="RHV13_000254"
     CDS             complement(6947..7792)
                     /gene="potI"
                     /locus_tag="RHV13_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HDV1239821.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            complement(7789..8742)
                     /gene="potH"
                     /locus_tag="RHV13_000255"
     CDS             complement(7789..8742)
                     /gene="potH"
                     /locus_tag="RHV13_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HDV1239822.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTICCLMIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(8752..9885)
                     /gene="potG"
                     /locus_tag="RHV13_000256"
     CDS             complement(8752..9885)
                     /gene="potG"
                     /locus_tag="RHV13_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HDV1239823.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLYSPGLV
                     HPLKVDVDASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(9980..11092)
                     /gene="potF"
                     /locus_tag="RHV13_000257"
     CDS             complement(9980..11092)
                     /gene="potF"
                     /locus_tag="RHV13_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HDV1239824.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATELLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(11443..11919)
                     /locus_tag="RHV13_000258"
     CDS             complement(11443..11919)
                     /locus_tag="RHV13_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HDV1239825.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(12007..12909)
                     /gene="rimK"
                     /locus_tag="RHV13_000259"
     CDS             complement(12007..12909)
                     /gene="rimK"
                     /locus_tag="RHV13_000259"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HDV1239826.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(12970..13692)
                     /gene="nfsA"
                     /locus_tag="RHV13_000260"
     CDS             complement(12970..13692)
                     /gene="nfsA"
                     /locus_tag="RHV13_000260"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HDV1239827.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPSSILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(13676..13963)
                     /locus_tag="RHV13_000261"
     CDS             complement(13676..13963)
                     /locus_tag="RHV13_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HDV1239828.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAVVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            14123..14380
                     /gene="grxA"
                     /locus_tag="RHV13_000262"
     CDS             14123..14380
                     /gene="grxA"
                     /locus_tag="RHV13_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HDV1239829.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(14410..14787)
                     /locus_tag="RHV13_000263"
     CDS             complement(14410..14787)
                     /locus_tag="RHV13_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HDV1239830.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            15057..16742
                     /locus_tag="RHV13_000264"
     CDS             15057..16742
                     /locus_tag="RHV13_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HDV1239831.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(16917..17453)
                     /gene="rcdA"
                     /locus_tag="RHV13_000265"
     CDS             complement(16917..17453)
                     /gene="rcdA"
                     /locus_tag="RHV13_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HDV1239832.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSRFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            17537..18745
                     /locus_tag="RHV13_000266"
     CDS             17537..18745
                     /locus_tag="RHV13_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1239833.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALVGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            18745..19560
                     /gene="ybjI"
                     /locus_tag="RHV13_000267"
     CDS             18745..19560
                     /gene="ybjI"
                     /locus_tag="RHV13_000267"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HDV1239834.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGNAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            19646..19930
                     /locus_tag="RHV13_000268"
     CDS             19646..19930
                     /locus_tag="RHV13_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1239835.1"
                     /translation="MFMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(19971..21203)
                     /gene="mdfA"
                     /locus_tag="RHV13_000269"
     CDS             complement(19971..21203)
                     /gene="mdfA"
                     /locus_tag="RHV13_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HDV1239836.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGQFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            21488..22084
                     /gene="ybjG"
                     /locus_tag="RHV13_000270"
     CDS             21488..22084
                     /gene="ybjG"
                     /locus_tag="RHV13_000270"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDV1239837.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            22142..22900
                     /gene="deoR"
                     /locus_tag="RHV13_000271"
     CDS             22142..22900
                     /gene="deoR"
                     /locus_tag="RHV13_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HDV1239838.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLASTLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGNVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(22947..24149)
                     /gene="dacC"
                     /locus_tag="RHV13_000272"
     CDS             complement(22947..24149)
                     /gene="dacC"
                     /locus_tag="RHV13_000272"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HDV1239839.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            24396..25022
                     /gene="gstB"
                     /locus_tag="RHV13_000273"
     CDS             24396..25022
                     /gene="gstB"
                     /locus_tag="RHV13_000273"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HDV1239840.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(25019..26134)
                     /gene="yliI"
                     /locus_tag="RHV13_000274"
     CDS             complement(25019..26134)
                     /gene="yliI"
                     /locus_tag="RHV13_000274"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HDV1239841.1"
                     /translation="MHRPFFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWSLAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(26245..26628)
                     /gene="bssR"
                     /locus_tag="RHV13_000275"
     CDS             complement(26245..26628)
                     /gene="bssR"
                     /locus_tag="RHV13_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HDV1239842.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            26841..28166
                     /gene="rimO"
                     /locus_tag="RHV13_000276"
     CDS             26841..28166
                     /gene="rimO"
                     /locus_tag="RHV13_000276"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HDV1239843.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(28213..29541)
                     /locus_tag="RHV13_000277"
     CDS             complement(28213..29541)
                     /locus_tag="RHV13_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDV1239844.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTQEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(29549..31897)
                     /locus_tag="RHV13_000278"
     CDS             complement(29549..31897)
                     /locus_tag="RHV13_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDV1239845.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            complement(32075..32986)
                     /gene="gsiD"
                     /locus_tag="RHV13_000279"
     CDS             complement(32075..32986)
                     /gene="gsiD"
                     /locus_tag="RHV13_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HDV1239846.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(32989..33909)
                     /gene="gsiC"
                     /locus_tag="RHV13_000280"
     CDS             complement(32989..33909)
                     /gene="gsiC"
                     /locus_tag="RHV13_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HDV1239847.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(33927..35465)
                     /gene="gsiB"
                     /locus_tag="RHV13_000281"
     CDS             complement(33927..35465)
                     /gene="gsiB"
                     /locus_tag="RHV13_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HDV1239848.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALENYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDNFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(35485..37356)
                     /gene="gsiA"
                     /locus_tag="RHV13_000282"
     CDS             complement(35485..37356)
                     /gene="gsiA"
                     /locus_tag="RHV13_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HDV1239849.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(37343..38308)
                     /gene="iaaA"
                     /locus_tag="RHV13_000283"
     CDS             complement(37343..38308)
                     /gene="iaaA"
                     /locus_tag="RHV13_000283"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDV1239850.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTETVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFTRGMERVSPEIFSTPLRYEQLMAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDAVATQ"
     gene            38512..39747
                     /gene="moeA"
                     /locus_tag="RHV13_000284"
     CDS             38512..39747
                     /gene="moeA"
                     /locus_tag="RHV13_000284"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HDV1239851.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLSNCFIVLERDRGN
                     VEVGEWVEVEPFNALFGGL"
     gene            39747..40496
                     /gene="moeB"
                     /locus_tag="RHV13_000285"
     CDS             39747..40496
                     /gene="moeB"
                     /locus_tag="RHV13_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HDV1239852.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(40675..41337)
                     /gene="fsa"
                     /locus_tag="RHV13_000286"
     CDS             complement(40675..41337)
                     /gene="fsa"
                     /locus_tag="RHV13_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDV1239853.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTTEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWQGAFGRT
                     SI"
     gene            41468..42367
                     /locus_tag="RHV13_000287"
     CDS             41468..42367
                     /locus_tag="RHV13_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HDV1239854.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAAFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            42373..44805
                     /locus_tag="RHV13_000288"
     CDS             42373..44805
                     /locus_tag="RHV13_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HDV1239855.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            44951..45766
                     /gene="ybiV"
                     /locus_tag="RHV13_000289"
     CDS             44951..45766
                     /gene="ybiV"
                     /locus_tag="RHV13_000289"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HDV1239856.1"
                     /translation="MSVKVIVTDMDGTFLNDVKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNIAPFNS"
     gene            45918..47177
                     /locus_tag="RHV13_000290"
     CDS             45918..47177
                     /locus_tag="RHV13_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HDV1239857.1"
                     /translation="MTFSCETLPTDAKAAIREMKAALREQLGDVQQVFDTLSAKIETR
                     VAEIDALKAQGLPVWPEVSYSDIAAGTVSDATRNEIKRRGCAVIKGHFPRELAMAWDR
                     AMLDYLDINHFDDVYKGPGDSFFGSLEASRPEIYPIYWSQAQMQARQSENMAAVQSFL
                     NRLWTSESNGKQWFNPDISVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQRV
                     FANVFNGNFDDYDPWDAAHRTDVEEYTVDNTTKCSVFRTFQGWTALSDMLSGQGLLHV
                     VPIPEAMAYVLLRPLLDDVPDDELCGVAPGKVLPISEKWHPLLLKALTSIPAITAGDS
                     VWWHCDVIHSVAPVDNQQGWGNVMYIPAAPMCEKNRAYAHKVKMALEKGASPGDFPRE
                     DYEANWEGRFTLADLNIHGKRALGMDV"
     gene            complement(47418..49010)
                     /locus_tag="RHV13_000291"
     CDS             complement(47418..49010)
                     /locus_tag="RHV13_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HDV1239858.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            49229..50149
                     /gene="ldtB"
                     /locus_tag="RHV13_000292"
     CDS             49229..50149
                     /gene="ldtB"
                     /locus_tag="RHV13_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDV1239859.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(50207..51325)
                     /locus_tag="RHV13_000293"
     CDS             complement(50207..51325)
                     /locus_tag="RHV13_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HDV1239860.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYFVFLTALE
                     LKQELWGLVIVAVGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWASLVGYVLLVMIPDW"
     gene            complement(51322..51789)
                     /gene="mntR"
                     /locus_tag="RHV13_000294"
     CDS             complement(51322..51789)
                     /gene="mntR"
                     /locus_tag="RHV13_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HDV1239861.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            51975..52103
                     /gene="mntS"
                     /locus_tag="RHV13_000295"
     CDS             51975..52103
                     /gene="mntS"
                     /locus_tag="RHV13_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HDV1239862.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            52375..53958
                     /gene="opgE"
                     /locus_tag="RHV13_000296"
     CDS             52375..53958
                     /gene="opgE"
                     /locus_tag="RHV13_000296"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HDV1239863.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEISIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(54007..54522)
                     /gene="ompX"
                     /locus_tag="RHV13_000297"
     CDS             complement(54007..54522)
                     /gene="ompX"
                     /locus_tag="RHV13_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HDV1239864.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(54575..54640)
                     /locus_tag="RHV13_000298"
     CDS             complement(54575..54640)
                     /locus_tag="RHV13_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1239865.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            54875..>55757
                     /gene="rhtA"
                     /locus_tag="RHV13_000299"
     CDS             54875..>55757
                     /gene="rhtA"
                     /locus_tag="RHV13_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HDV1239866.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDI"
BASE COUNT        13886 a        14648 c        13640 g        13583 t
ORIGIN      
        1 ttttcaaaaa tacaacgtaa ttttggcaat catcttaata gcaggatctc ttatcgcatg
       61 tgaaaaattt gtaatgatta gacaataacc gcgttcattt atcacgcgtt ataaatgtga
      121 aactggcttc ttgcagatgc tcttaacctc tacaggagga tttataccac catttcagaa
      181 ctatcatatt taccttaaat gatcatttca gccatataac acgctcaacc tcacaaatgg
      241 tttacactgt ctattgtttg ggtaatttaa gtaagtcagc tacgagcaac cttgagtggg
      301 tagccagcaa tggactcaaa cccaatcagg tgttcagatt gttttagaat tgtattaaaa
      361 acaatgtatt aaataatctg atagcgtttg gtcgtagtgt gacgcagaca gcaagctaat
      421 aaggaaatga ttatgcgcta ccgttcacta tctctactta ttccatgtgc actgctgctg
      481 agtgcctgta ccaccgtcac gccagcttat aaagataacg gtccccgcac cggttcctgt
      541 gtccaggggg ggcctgacag cgtggcccaa caattctatg actaccgcat tcaacatcgc
      601 agcaatgaca ttaccgccct gcgcccctat ttgagtgaca aactggcaac cctacttagc
      661 gatgccagcc gcgataatag tcatcgtgaa ttgttaagca gcgatccgtt ctccagtcga
      721 accaccttgc cagacagcgc ccacgttgcc agtgcatcga ctatccctaa tcgtgatgcc
      781 cgtaacattc cattgcgcgt ggatctcaag cagggtgacc agggctggca ggatgaagtg
      841 ttaatgattc aggaaggcca gtgttgggtg atcgacgatg tgcgctactt aggcggtagc
      901 gttcatgcca cggcgggtac gctccgtcag tccatcgaga atcgctaacg ccttgatcaa
      961 ccaataaacc ccgtaaattg tccggcattt cgtgaggaat gccgacattt atgctcgccg
     1021 accaccgccc ccgttatttt gtgctatgtt tattgaataa tgcgctttgc ttttaacttt
     1081 taaagcagaa atattgcata attattctgt caaaggtact atcttcgacc tcaatagcta
     1141 tcagactgcc agtatacgag tgtcaatgag tattcaatta aacggcatta attgcttcta
     1201 cggcgcgcat caggcgctgt tcgatatcac gctggattgc ccacagggcg aaacgctggt
     1261 gttacttggc cccagcggcg cgggtaaaag ctcgctgctg cgtgtactca atctgcttga
     1321 gatgccgcgc tccggtacgc tcaacattgc aggcaaccat ttcgatttca ccaaaacacc
     1381 ctcagacaaa gcgattcgcg atttgcgtcg taacgttggc atggtgtttc agcaatacaa
     1441 cctgtggccg catctgaccg tgcagcaaaa cctgattgaa gcgccctgcc gtgtactggg
     1501 gttgagtaaa gatcaggcgc tggcccgtgc agaaaaactg ctggaacgtc tgcgtctcaa
     1561 accttatagc gatcgttacc cgctgcatct ttctggtggt cagcagcagc gtgttgctat
     1621 tgcccgtgcg ttaatgatgg aaccgcaggt actgctgttt gatgaaccga ccgccgcact
     1681 ggacccggaa attaccgcac aaatcgtcag catcattcgt gagctggcag aaacgaatat
     1741 tacccaggtg atcgtcaccc acgaagttga agtggcgcgt aaaaccgcca gccgagtggt
     1801 gtatatggaa aatggtcata tcgtagaaca aggcgacgcg agctgcttta ccgagccgca
     1861 aaccgaagca tttaaaaact atctctctca ctaagattcg ggataacaac aatgaaaaaa
     1921 gttctgattg ccgcgttaat tgcaggtttt agtctttccg ccacagctgc cgaaaccatt
     1981 cgttttgcta ccgaagcctc ctatcctccg tttgaatcga ttgatgcaaa caaccagatc
     2041 gttggttttg acgtcgacct ggcacaggca ctgtgtaaag agattgatgc gacctgtact
     2101 ttctctaacc aggcgtttga cagcctgatc ccaagcctga aattccgtcg cgtagaagcc
     2161 gtgatggcgg gcatggatat cactccggag cgtgaaaagc aggtgctgtt taccaccccg
     2221 tactatgaca actctgccct gtttgtgggt cagcaaggca aatacaccag tgttgatcag
     2281 ctgaaaggca aaaaagtcgg cgtacagaac ggtacgacgc accagaaatt cattatggat
     2341 aagcacccgg aaatcaccac cgtgccgtat gacagttacc agaacgcaaa actggatctg
     2401 caaaacggtc gtatcgacag cgtatttggt gacaccgcag tggtaaccga atggctgaaa
     2461 gataatccga aactggcggc tgtgggcgac aaagtgaccg acaaagatta cttcggcact
     2521 ggcctcggca ttgcggtacg tcagggcaac actgaactgc agcagaaatt caacgctgcg
     2581 ctggaaaaag tgaagaaaga tggcacttac gaaaccatct acaacaaatg gttccagaag
     2641 taattcctga tgaatgaatt ttttccttta gcaagcgccg ccgggatgac cgtcggcctt
     2701 gccgtttgtg cattgattgt cgggctggcg ctggcgatgt tctttgcggt atgggagtcg
     2761 gcaaaatggc gtcctgtcgc gtgggcaggt tcagcgctgg taaccattct gcgtggcctg
     2821 ccagaaattc tggtggtgct gtttatctat tttggttcct cgcagctgct gctgacgctt
     2881 gcggatggct tcactatcaa tcttgggttc gtgcagatcc cggtgcagat ggacattgag
     2941 aacttcgacg tgagtccgtt cctttgtggt gtcatcgctc tgtcactgct gtatgccgcc
     3001 tatgcctcgc aaacgctgcg cggcgcgttg aaagcggtac cggtgggcca gtgggaatct
     3061 ggtcaggcgc tggggctgtc gaaatcggct atctttttcc gtctggtgat gccgcagatg
     3121 tggcgtcatg cgctgcctgg cctcggtaac cagtggctgg tgctgctgaa agataccgcg
     3181 ctggtcagtt tgattagtgt gaatgattta atgctacaaa ccaaaagcat tgctactcgt
     3241 acccaggaac cttttacctg gtacattgtg gcggcggcga tttacctggt gatcaccctg
     3301 ctcagtcagt acattctcaa acgcattgac ctgcgcgcga cacgttttga gcggaggccc
     3361 agctaatgtt tgagtattta cccgaactga tgaaagggct gcacaccagc ctgacgctaa
     3421 ccgttgcctc gctgattgtg gcactgattc tggcattgat ttttaccatc atcctgacgc
     3481 tgaaaacgcc ggtgctggtg tggctggtgc ggggttatat cacgctgttt accggtacgc
     3541 cgctgctggt gcagatcttc ctgatttact acgggccggg ccagttcccg acgttgcagg
     3601 aatatccggc actgtggcat ttgttgtcag aaccgtggtt atgtgcattg attgcgttat
     3661 cgctgaacag tgcggcgtac accacgcagc tgttttacgg tgcgattcgt gcgatcccgg
     3721 aaggtcaatg gcaatcctgt agcgctctgg gaatgagtaa aaaagataca ctggcgatcc
     3781 tgctgccgta tgcctttaaa cgctcactct cttcttattc caacgaagtg gtgctggtgt
     3841 tcaaaagtac ctctctggct tacaccatta cgctgatgga agtgatgggt tacagccagt
     3901 tgttgtacgg gcgcacctac gatgtgatgg tatttggcgc ggcggggatt atttacctga
     3961 tcgttaacgg cctgctgacg ctgatgatgc gtctgatcga gcgcaaagca ctggcattcg
     4021 aacggcggaa ttaatgtagc tgcgtacatt cgccgggggg tttgtaggtc tgataagacg
     4081 cgccagcgtc gcatcagaca tcagcacggt gcctgtgccg gatgtggcgt ggacgcctta
     4141 tccggcctac atagtgcata aattcttata atgaagacgg acaactcact aagttgtccg
     4201 tcttttttta tttcatttaa attatttaat catatttatt gcatataaat tcacttaatg
     4261 gcattgttat cccatgccgc agacacggcc aaaaatcata agattgacag acgggagttc
     4321 catcatgaaa aagttagttc ttgccgcatt acttgcttcc tttactttcg gtgcttctgc
     4381 cgcagagaaa atcaattttg gcgtttcagc cacctatcca ccctttgaat ctataggtgc
     4441 taataatgag attgtcggct ttgatatcga tctggcaaaa gccttgtgca aacaaatgca
     4501 ggcagaatgt acttttacta atcacgcgtt cgacagcctg atcccgtcac tgaaattcag
     4561 aaaatatgac gccgtaatct ccggtatgga tatcaccccg gagcgtagca agcaggtttc
     4621 gtttaccaca ccctactatg aaaactcagc cgtcgtgatt gccaaaaaag atacctacaa
     4681 aacgtttgcc gacctgaaag gcaaacgcat tgggatggaa aacggtacga cgcaccagaa
     4741 atatattcag gatcagcacc cggaagtgaa aactgtctcc tatgacagtt atcagaatgc
     4801 ctttatcgat ctgaaaaatg gtcgtattga tggggtattt ggtgacaccg cggtggtaaa
     4861 cgaatggctg aaaaccaatc cgcaactggg cgttgcgact gaaaaagtga ccgatccgca
     4921 atacttcggc accggcctgg gtatcgctgt acgtccggat aacaaagctc tgctggaaaa
     4981 actgaataac gcgctggcag caattaaagc tgacggtaca tatcagaaaa tcaatgacca
     5041 gtggtttccg cagtaattga gactgatgac aaaagtaaga ttgcctgatg cgctgcgctt
     5101 atccggccca catcaactct gcaatgtatt gatttagcat gattttgtag gccggataag
     5161 gcgttcacgc cgcatccggc atggacaaag cgcacctgat cagcaatctg aagccgcacc
     5221 tgtggctttt ttattgcttc accagcagcg tcagcacttc atagtgcgcg gtgtgcggga
     5281 acatatcaaa aagctgtacc cgttcgatgc gataaccagg cagttcgcgg atatctttcg
     5341 ccatggtttg ggcgttacag ctggagtaga tgataaaacg cggtgccatc gttgagagat
     5401 aatcacacag cggtttacca atgccgcggc gcggcgggtt aaccagcacc agctccggca
     5461 cctccccctg agcggtggca aactgagtgg agtccagcgc ctgaaattgc aaacgcgtta
     5521 agcccagttc agcggctgac tgctttgcac aggcaatggc ctctggcgca atttcgatcc
     5581 cggttaactg catgtcaggc gtcgcgcagt gtaaaccaaa gccccccacg ccacagaaca
     5641 gatcccacat atgtgtaacc ggcagttgtc gtacccagtc gcgcgcggta gcgtacagct
     5701 ggctggcgac cgccggatta gtctggaaga aactttgcgg acggatccac agcggtacgt
     5761 cattaaaacg ctctgccagt gcctgatgtt cggtcaggta gatctccgtc tccccttcca
     5821 taatcgccat atgtaccggc tgaatattga cggtaataac tttcagctgc ggtagttgtt
     5881 cgtgtaacca cggcagcgcc ttacgcagtt gcgccagctt ggtatcagaa cgcagtacaa
     5941 agcgcagcat catgccgcca tcgctctggc tttcagtcag cagaatgtat ttcagttcgc
     6001 cacgcttacg cgccacgttg tagggcgtta accccgctcg ggcgataaac ggttttagcg
     6061 ccgcaaaaac gggcgcaaat gaggcaggat aaagcgggca gtcacaaagg tcttccggtg
     6121 tgccgtctcg atgcagcata ccgagcagtg gtttttcaac gctaccactc accaccattt
     6181 tggctttatt acgaaaccct tgttgcgggc ctgacaccgg cgcgcaccat tcctcaaccg
     6241 gaaagtcggc gagcagattt ttaagatcgg cggttttagc ggagagttgc tctggaatcg
     6301 gctgcgttat ccactgacag gaacgacagc gacccgcgtc gtaaagtgcg cactgcatac
     6361 attgaccttc acatcatcag gggcgacgat tatacacatt attgcaactg gaagaaccgc
     6421 cgactggtcg agggaacgaa cagcagcatc aggatgagca tatccggcag cttctgcagc
     6481 atcaggctat ggaagatttc acgttttgat tcaccgggaa tgctgaacag ctccggataa
     6541 ccgtacccca gcgaagccgc ccacagatag ctggcggccg tgacttgagt cagcagatac
     6601 agccagcgcg cccagcgacg ccctttcacc agtgaaaacg cacaccagat ctcaataaaa
     6661 accagcacca gactgcttaa aaagaccagc gttaaactcc aggtttgtac gctacgatga
     6721 atgaactcgc caataccgcg cacgcccagc gtattgaaaa tcatcagcac gtcgaggcca
     6781 cggatcataa taatggcgag cgccgccacc tgcaccagcg caggcacatt caggcgagca
     6841 tgagatgaag atgttttctt aaaaaatccc aacgtttcgt cttccatgaa aacaatgccg
     6901 cgacatgcgc ggcattatgt agccaggttg gcaaatttta gtgtcttcag ccacgtcttg
     6961 cacgctggat gtcgcgtatc cgctgttttt ctgcgcgagc catcagatac caggcgataa
     7021 atccgacaat tccgaccgcg ccgaggatca atgttgccag ggcgttgatt tccggattca
     7081 cccccatccg cacgctggaa aagaccagca tcggtaacgt ggtggctccc ggcccggata
     7141 caaagctggc gatcaccaga tcatcaagcg acaaagtaaa agccagtaac cagccagaaa
     7201 tgatcgcggg catgatcatc ggtagcgtaa tgacgaaaaa cactttcagc ggcgtcgcac
     7261 cgagatccat cgctgcttct tctatcgagc gatccagttc ccgcagacgc gacgaaataa
     7321 cgactgccac ataagccgta cagaaagtga catgcgccag ccagatggta agcataccgc
     7381 ggtccgcagg ccagccaatg gcatgagcaa gcgcgacgaa taataacaac agcgacaagc
     7441 ccgtgatgac atctggcatt accagcggcg cggtgatcat aaaggcaaag ccatttgatc
     7501 cgcgaaacct gccaaagcgc accagcacca ccgccgcaat agttccgagg atcgccgccg
     7561 ccgttgccgc acaggcagca attgtcaggc ttaaaccaac cgcactcatc atcgcgtcat
     7621 cgcgcaataa ctcaccatac cagcgcgttg accagccggc ccacaccgtc accagcttcg
     7681 agctgttaaa cgaatagata accagcatca gcattggcgc gtagagaaag gtaaagccca
     7741 gcagcaaaat cacaatccgc cagggcgaac gaactaccgg taaattattc atccgtgttc
     7801 tcccacgctt ttttgctggt gtttgtggaa ccacattatc ggcacaatta gcagcagtaa
     7861 catgatgatc gctaccgccg aggccaccgg ccagtcgcgg ttattgaaaa actcttgcca
     7921 tagcacgcgc ccgatcatga tgctgtccgg gccaccgagc agttccggga tcacaaactc
     7981 gcccacagcc gggataaaca ccagcatcga tccggcaata atcccacctt tagtcagcgg
     8041 cacgatcaca gtaaagaacg ttttcagcgg tcgtgcaccg agatccagcg ctgcttccac
     8101 cagcgaataa tcaatacgaa tcaacgcggt ataaatcggc agcaccataa acggcacgta
     8161 ggcgtaaaca atgccaatat aaactgccag attggtatga agaatggtca gcggttgatc
     8221 gataaccccc agccacagca gaaaattatt cagcacaccg ttgtttttta atattcccat
     8281 ccaggcgtag acacgaatca aaaacgaggt ccacgacggc aggatcacca gtagtaataa
     8341 gatattacgg gtcgaaggct tactgtgcgc caccgcccac gccagcggat agccgatcat
     8401 taaacagcaa atagtcgaaa tcgccgccac ctgtaacgac tggagataag catcgaaata
     8461 gagcggatcg tcggtcagtt gcagaaaatt accgagatta agagtgatgg acagttgccc
     8521 gtcagcccac tccatcagtt cggtataagg tggaatagcg cgcgccatct ccgccaggct
     8581 tattttaaag acaatcagaa atggcagcag aaacagcaag atcaaccaga tatatggcaa
     8641 cgcaatgacc agtttgcgcc catgcttcat ttgcagctgc gacaaccaca gcttaaatcc
     8701 gcccggcggt ttcgactggg cagcaggttc aagtgtactc atctttgctc cttaaaccgt
     8761 cagcaccaca cagctgtcca cttcccagca caaacgcact tcgtcgcccc aggtcggtaa
     8821 ccctttacga tgacgatggg cgttttgtag ctgggcgcta atcatctgcc cacttttcag
     8881 acgaacgtga tacaccgaaa gatcgccgag ataggcaatg tgtatcacct cccccaccgc
     8941 gaagttacaa ccattggcgg gcggctcttc gcaaagcatg attttttccg gacgcagcgc
     9001 tacgtgtacc ggcacgttat cgaccactga agcatccaca tcgactttca gtggatgcac
     9061 cagccccggc gaataaagca ccagaccatc ttcctgacgc tctttgagta caccttcaaa
     9121 gacattaacc gagccaataa attcggcgct atagcgggta gtcggatgct cgtagatctc
     9181 ttccggttcg ccaatctgga caaatttccc acgattcata atggcgatgc gccccgccat
     9241 ggtcatcgcc tcttcctgat cgtgggtgac catcacacaa gtcacaccga cgcgctccag
     9301 aatatccacc acttcaagct gcatccggtc acgcagcttt ttatccagcg cgcccatcgg
     9361 ctcgtcgagc agtaatagtt tcgggcgttt ggcaaggctt cgggccaggg ccacacgctg
     9421 tcgctgacca ccggaaagct gatgcggttt gcgtttggcg aactcctgca tatgcacaag
     9481 gccgagcatc tcattgaccc gactggcaat ttccgctttc ggtagtttgt cctgtttcag
     9541 gccaaaagcg atgttctgtt ccacggtcat atgcggaaac agcgcgtaag actgaaacat
     9601 catattgata gggcgcaggt aaggcggtac ctgtgacaaa tcgacgccat cgagcataat
     9661 ctgcccggca gaaggttgtt cgaaacctgc cagcatacgc agcagcgtgg acttgccaca
     9721 gccggatgcg cccagcagcg cgaagatttc acctttgtag atggtcaggc tgacatcatc
     9781 caccgcatgt tgaccatcgt aggatttggt gaggttgcgg atttctaata gcggcgtcag
     9841 cgccttacgg gttttcgcct gcgggcgagg gatagcgtca ttcacggctg ctctccggca
     9901 ttgaatgtgt gcaagcgcac catgatggtg cgaattgccg gcgggtgtgg tgcgcccgcc
     9961 cggcatctaa ggctgcggat tattttccgc tcttcacttt ggtccacgcg cgggtgcgca
    10021 cacggtcgat tttcggatcc tgtactttca gagtgaacaa cttcgcgcgg acatccgcag
    10081 gcggataaat acccgggtta tcacggactt ccgcacttac cagcggcgtg gctgctttat
    10141 tggcgttagc atagaacaca tggtcagaga tatgtgccac cacatcagga cgcagcaggt
    10201 aattcaggaa ctgataggct tcgtctttat ttttggcatc cgcaggcatg gcgaatacat
    10261 caaagaacgc catcgcccct tcttttggaa tcgagaacga gacattcacg ccattcttcg
    10321 cttccttcgc gcggtttgac gcctgccaga catcacctgc ccagccgata gcgacgcaaa
    10381 tatcgccgtt tgccaggtcg ttaatgtatt gagatgaatg gaaataacga atgttcgggc
    10441 gcagctttaa cagcagctcg gtagccggtc cggtgtaatc atccgcttta gtgctgtttg
    10501 gatctttgcc gagataattc agcacggtag caaaaacttc ttctggcgca tcaaggaaag
    10561 atacaccgca gcttttcagt ttttccagat tttcaggctt gaggatcaag tcccagctat
    10621 cgacgggcgc gttttcgccc agcaccgctt tgactttatc gacgttatag ccaatcccgg
    10681 tcgtcgccca catatagggc atagcaaatt tattgtcggg atcgtgtttg gcgaccaact
    10741 tcagcagttc cgggtcgaga ttcttccact ccggcaattt gcttttatcc agcggctgga
    10801 aaactcccgc tgtcagctgg cgttcaagaa agcttgctga cggcaccacc aggtcaaagc
    10861 cggtgctccc ggccattaat ttgccttcca gtacttcgtt agagtcgaaa acatcgtaga
    10921 cgactttaat accggtttct ttttcaaagt tagccaccgt gtcgggggcg atataatcag
    10981 accagttata aatgtggagt gttttttgtt cagccgcgag cgtgccgaca gagacggcca
    11041 tcagagcacc cgcaaccaga cccgaaagcc attttttatt taaggcggtc atatctcttt
    11101 ccttctgaaa gttcgttaac aaacgaacaa aagttgtcac actgaaagat atgcaataaa
    11161 cgtgcatatt ttttatcggc gcggtgtaag aaaagagaac tctctcccgg ccagcactgc
    11221 tcgctatata aaagcatcgc aagaataact ttagccaggg tttgagacta tagctcggat
    11281 taaaaaacgg tatttaacaa atttttatgc gtatttgctc tatgtgataa agccaaatcg
    11341 acacaagaga agtgaaataa tctttaagaa aaggttaaaa acagcagata attttgcagc
    11401 gcgcagccac tgcaccagcg actgcgtgct cgaaaggagt tatcagtgga ggaaatagtt
    11461 ttcggtaacg ttgttttgcc cttcatcgtc agtcggtaac agcagttgat gggcattagc
    11521 ttcaagaatg accatcgaaa tctgctcttc gctctgacgc acgaaccatg cgaactgctc
    11581 ataggttacg ccctgcataa cggataaaga ctggcaaacc accagctttg gcagattatc
    11641 atcctgcata tcaagaaatg ctttcacggt cagcgaactg gcattgatgg cagataaatc
    11701 cgccgccagc ggcaatacgg ctgaaggtcg gacttccgcc atggcagaaa acaggatcgt
    11761 gttatcgatc agatcgattt tggcatcaaa gacaccgtcg aaattctgca tatggggcag
    11821 atgcagagcc tgacagttat cacattcaaa aaaactcatc cccaggtcat cgagccattg
    11881 acgcagcgta tccagaccag gaacgaccag cgatgtcata attttgtaca acctctttcg
    11941 ataaaaagac cggcacagct tacgcaaaaa gcgcaggcaa aaccatgatc agtaatgtga
    12001 ttgcgactaa ccacccgttt tcaggcaata ttctgtcgta gcgtggcgtt cgatccagcg
    12061 gatcatttta cccgcgatgt cgataccggt cgttttttct attccttcca gccccggcga
    12121 cgcattcact tccatcacca acggcccgcg attagcacgc agaatatcca caccagcaac
    12181 gtccagcgcc atcgttcgcg cggcttttat cgcgatttca cgctcctgtg gtgtgatact
    12241 ggcgacgctt gccgcgccgc cacgatgcaa attggaacga aaatcgccct ctttcgcccg
    12301 ccgttcaatc gcagcgacca cttcatcgcc aacaaccaga cagcggatat cgcacccttg
    12361 cgcctctttg atatattcct gcaccagaat atgcgcgttc agaccgcgga aagcgtcaat
    12421 cacgctttcc gccgcctgac gcgtctccgc cagcacgacg ccaattccct gcgtgccttc
    12481 aaccaacttg accaccagcg gcgcaccacc gaccatgtcg attaaatcgc tggtatcatc
    12541 cggcgaatgc gcaatgcccg tgacaggcag gtcgatgccc tgacgcgcca gcagttgcat
    12601 ggaacgcaat ttgtcacgcg cccgggcaat ggcgaccgac tcattgagcg gatagctccc
    12661 cagcatctcg aactggcgca gtgccgccgt cccataaaag gtaatggcgg tgccaatacg
    12721 cgggatcact gcgtcaaaat ggggtaactt gcggcctttg tagtgaatag aagacgccgc
    12781 aggatttatg ttcatgtagc aagaaagcgg atcaagaatt tcaaccaggt gaccgcgctg
    12841 tatagcggct tcacgcagcc gcttacacga atagagcgtt ccatcccggg acaatatggc
    12901 aattttcacc ctgcacctct ctgtcagacc tggtcaaaag cccgcgtatc atacatcgac
    12961 gtggcggttt tagcgcgtcg cccaaccctg tttgtgcaaa taatccagaa taaatgggcg
    13021 gctttcttta atgattgttc ggcggatatg atcgctccag gtatcccggc gattattgct
    13081 gccacgggtg aggtaatatt ccgccagttg ttcgtcatac tgcgccagtg cgtctttatc
    13141 cagcggttga tagctgtttt catgcaccaa aatggaggac ggtaaacgcg gcttaagatc
    13201 cggattatcc gcaggccagc caaggcacag cccaaacagc ggcagaacat gctgcggtaa
    13261 tttcagcagt ttcgtcaccg cttcaatatt attgcgcagg ccgccgatat atacgccgcc
    13321 caatcccagc gattccgctg cggttaatgc attctgcgcc atcattgccg tatcaacgac
    13381 accgagcaac agctgttccg ccaggccgag ctgagcatcc ggacagatct gtaaatggcg
    13441 gttaaagtcg gcacagaaca cccagaactc cgccgcttgc gctacgtgtt tttgcccgcc
    13501 ggtcaacgtc accagctctt cacgtaatgc tttgtcggta atgcgaataa tgctactaca
    13561 ctgcaaaaaa ctggaactgg acgtcgcacg ggcgctgtta ataatcgcct cacgctgcgc
    13621 ttcggaaatg ggttcatcag tgaaatggcg aatggagcga tggccacaaa taagttcaat
    13681 ggttggcgtc attatctttt tctctttctg aacgtgaata ttgcggtgga cggttcatca
    13741 gctgtggggc aagacgtttt gctacctgaa gaataaccac caccacagcg ggaagcatga
    13801 gcaaaacacc gagaaaaatc atcagaatct gcacttctgg ccgagaaaat ggctcaggca
    13861 gcgacaggga gtcgcttacc gacagcagcg ccaccgccag caacatcatt ccgataaatt
    13921 ccagtatcaa cacgctttta ggcaatttac cgatcgcgcg catacgcttc cctctgcaaa
    13981 gtgagccttc agtctaaaac ttttcacttt attgtgttta acagttatag cttttagcaa
    14041 ttaatgcaac aggttaaacc tactttcagc gaatacattt tagcgtgatc attacaggca
    14101 taaatctatg aggagagaaa taatgcaaac cgttattttt ggtcgtccgg gttgccctta
    14161 ctgtgtgcgt gcaaaagatc tggctgagaa attgagcaat gaacgcgatg attttcagta
    14221 tcagtatgta gatattcgtg cggaagggat cactaaagaa gatctacaac aaaaggcagg
    14281 taaacccgta gaaaccgtgc cgcagatttt tgtcgatcag caacatatcg gcggctatac
    14341 cgattttgct gcatgggtga aagaaaatct ggacgcctga tcgtctgaca agccctcgcg
    14401 ttgagggctt tactgatttt ttctgtgctg tggtttaaac aaactactga taaataagaa
    14461 acacagtgcc cccagcgcac accagaacac cgcgcttagt aaccatgcca gctcttgcca
    14521 gaatgagcgg gtcggtgaaa aaaacagccg cataataagc attgaacagg gtgccgccag
    14581 cattgcgcca aatagaggtt tcaggacttc tctacgctgt gaaaagaaac tggcaactgc
    14641 tccaggaaga atgaaaaaca gcaagccgat ttcaggatgc ccggcagccc gaaaagcgcc
    14701 tttcatgtgc gtcgccagaa aaaggcacac cacaatgaag aggacaaaac agcagattgc
    14761 ccccgcccaa cgttgtttat gtttcactcg ttcctcctga cactgcgtct atcgaacaca
    14821 tttttcgcca gtgtggcgtt cagtaagata aagccgcttc gcattccatg ctaatatagg
    14881 ccaacgcaat tcatatagcc gttgatacct aatgtgatta cactagtaaa atatattgtt
    14941 actttactat cgtttaggtg cgctgaatga atctgcgccc tgaattctgg taaaaaacat
    15001 tatcgtaaat taccatttct ttcaacagct tactagtaaa caagaagtta gcctccgtga
    15061 atataaacgt cgccgaattg ttaaatggga attacattct gttattattt gtggtcctcg
    15121 cgcttgggct atgtctcgga aagttacgac ttggttcgat ccaactgggt aattccattg
    15181 gcgttttagt cgtatcgctg ttattgggcc aacaacattt cagcattaac accgatgcgc
    15241 ttaatcttgg ctttatgctg tttattttct gcgtcggggt cgaagccgga ccgaactttt
    15301 tttccatttt ttttcgcgat gggaaaaatt acctaatgtt agcactggtg atggttggca
    15361 gtgcgctggt gatcgcctta gggttaggta agctgtttgg ctgggatatt ggcctgacgg
    15421 ccggtatgtt agcaggctct atgacgtcga caccggttct ggtcggtgct ggcgatacac
    15481 tgcgtcattc cggcatggaa agcaggcagc tctcactggc actggataat ctgagcctcg
    15541 gatatgcctt aacctattta atcggtctgg tgagtttgat tgttggtgcg cgttacttgc
    15601 cgaaattgca gcatcaggac ttacagacca gcgcccagca aatcgcccgc gaacgtggcc
    15661 tggacactga tgccaaccgt aaggtttatt taccggtgat ccgcgcctat cgcgtcggcc
    15721 cggagctggt ggcctggacc gacggcaaaa atctgcgtga actgggtatt tatcgacaaa
    15781 ccggctgcta cattgaacgt attcgacgta acgggattct ggcaaatcca gacggtgatg
    15841 ccgtgctaca aatgggcgat gaaatagcgt tggtaggcta tcccgacgcc catgcccgac
    15901 tcgatcccag cttccgtaac ggcaaagaag ttttcgatcg tgaccttctc gatatgcgta
    15961 tcgtcactga agaagtggtc gttaaaaacc ataacgctgt aggtaaacgt ctcgcacaac
    16021 tgaagttgac cgatcacggt tgcttcctta accgcgtcat tcgtagccag attgagatgc
    16081 cgatagatga caacgtcgtg cttaacaaag gtgacgtttt acaagtcagc ggcgatgccc
    16141 gccgcgtaaa aaccatcgcc gaccgcatcg gctttatctc gattcacagc caggtcactg
    16201 acctgctggc attctgcgcc ttctttgtta ttgggctgat gatcgggatg atcaccttcc
    16261 agttcagcac attcagtttc ggcatgggga acgctgccgg gttgttattc gccggaatta
    16321 tgctgggctt tatgcgtgct aaccacccga ccttcggtta cattcctcag ggtgcattaa
    16381 gcatggtgaa agagttcggc ttaatggtgt ttatggcagg cgttggtctg agcgccggta
    16441 gcggtattaa taacggtctg ggcgcgattg gcggtcagat gttgattgcc ggattgattg
    16501 tcagtcttgt gcccgtggtt atctgtttct tgttcggtgc ttatgtattg cgaatgaacc
    16561 gcgcactgtt gttcggcgca atgatgggcg cacgcacctg cgcgccggca atggagatca
    16621 tcagtgatac agctcgcagt aacatcccgg cgctgggcta tgcgggcacc tacgcaatcg
    16681 ccaacgtcct gctgacgctg gcagggacaa tcatcgtcat ggtatggcca ggattaggat
    16741 aaaactgaag ttgccctgaa aatgaaattt ttttgcacaa ccgcagaact tttccgcagg
    16801 gcatcagtct taattagtgc cactgctttt ctttgatgtc cccattttgt ggagcccatc
    16861 aaccccgcca tttcggttca aggttggtgg gttttttgtt gcctgaaatt tatctactac
    16921 cctgcaaccc tttcaaccat cctcaaaatc tcctcgcgcg atagcggctt acggtcggtg
    16981 acaaaatgca gcgtcatccc ctcaataaac gcatcaagcg cgcgggcggt tccgggttca
    17041 aaccattgtt cgagcgtttg ctgactgcgc tgcatccagt tttgcattac cgtttttaat
    17101 agcggttttc ggctagccag cgcgtagagc tggtacatca gctccatgtt atccggcgtt
    17161 gcaacctgtg agctgtagat catatcggtg atagcctggc atgcgccttg agcatcacta
    17221 acatcgctaa aaaatgcctg atattgccgg gacatgatct cagtaaaacg gctgaacgcc
    17281 tccagcaaca actcatcaat tcctgaaaaa tagtaggtca tcgatcccaa cggtaccccg
    17341 gcaagggtag caattttgcg gtgcgtaaca gcatgtattc cgtaaagttt caccgcctcc
    17401 agcgtggcct ggataatttt ttctcgccgt tgcggatcgt tagcgcgacg catgtcattt
    17461 cctctgtctg caattgtgta caaatgtaca caaccttgct aatgttgtct tccctcttct
    17521 tattttgacg tgatctatga ccgtaaattc ttcacgtaat gcattgaaac gccgaacctg
    17581 ggcgctgttt atgttcttct ttttgccggg cctgttaatg gcgtcctggg caacccgtac
    17641 gcctgctatc cgcgatattc tctctgtctc gatcgctgaa atgggcggtg ttctctttgg
    17701 tctgtcgatc ggttcgatga gcggtattct ctgctcggcg tggttagtga aacgctttgg
    17761 gacacgtaat gtcatcctgg tcaccatgtc ctgcgcattg gtcgggatga tgatattaag
    17821 tctggcactc tggctgacat cgcccctgct ctttgccgtt ggtctcggcg tctttggggc
    17881 aagttttggt tctgcggaag tggcgataaa cgttgaaggt gccgccgttg agcgagaaat
    17941 gaataaaacg gttttgccga tgatgcacgg tttttatagc ctgggcacgc tggcaggcgc
    18001 tggtgtcggg atggcactga cggcctttgg cgttccggca acggtgcaca ttttattggc
    18061 ggcgctggtg ggcatcgcgc ctatatatat cgccattcag gcaatccctg acggtacggg
    18121 caaaaatgct gccgatggca cccagcatgg cgaaaaaggc gtaccttttt atcgcgatat
    18181 ccagttgctg ctgattggtg ttgtggtgct ggcgatggcc tttgccgaag gttctgccaa
    18241 cgactggtta cccttattaa tggttgacgg tcacggtttt agccccactt ccggctcgct
    18301 gatttatgcc ggttttaccc tggggatgac cgttggacgc ttcactggcg gttggttcat
    18361 cgaccgttac agtcgcgttg ccgtggttcg ggccagtgcg ctaatggggg cgttgggtat
    18421 tgggctgatt atttttgtcg atagcgcctg ggtcgctggg gtgtctgttg tactctgggg
    18481 actgggtgcc tcgctgggct tcccgctgac catttctgcc gccagcgata ccggccccga
    18541 tgcaccaacc cgcgtcagtg tggtagcaac gaccggttat ctggctttcc tcgtcgggcc
    18601 gccgctgctg ggctatctcg gcgaacatta tggattacgt agtgcaatgc tggttgtact
    18661 ggcgctggtt attctcgcgg ctattgtcgc gaaagccgtc gccaaacccg ataccaaaac
    18721 gcagacggcg atggagaata gttgatgagc attaaattaa ttgcggtaga catggatggt
    18781 actttcttaa gcgatcaaaa aacctataac cgtgagcggt ttatggctca gtatcagcaa
    18841 atgaaagcac aaggcattcg ctttgtggtc gccagcggaa atcaatatta tcagttgatc
    18901 tctttcttcc ctgaaattgc taatgaaatt gcctttgtgg ctgaaaacgg cggctgggta
    18961 gtgagtgaag gcaaagatgt ttttaatggc gagctgtcga aggatgcgtt tactactgtc
    19021 gtggaacatt tgctgacgcg cccggaagtg gaaattattg cctgcggaaa aaatagcgcc
    19081 tatacgctca aaaaatatga cgatgccatg aaaacggtgg cggaaatgta ttatcaccgt
    19141 ttggaatacg tcgataactt tgacaactta gaagatattt tctttaagtt tggcctgaat
    19201 ctttccgatg aactgattcc acaagtacaa aaagcattac atgaggccat cggcgatatt
    19261 atggtgccgg tccacaccgg caacggcagc atcgatctga ttatccccgg cgtacataaa
    19321 gccaatggcc ttcgccaact gcagaaatta tggggaatag acgacagcga agtggtggtc
    19381 tttggcgatg gcggtaacga tattgagatg ctgcgtcagg caggctttag ttttgcaatg
    19441 gaaaatgccg gcaacgcggt cgtcgcagcg gcaaaatacc gggcaggctc caataaccgt
    19501 gaaggcgtac tggatgtgat cgataaagtg cttaaacacg aagcgccatt tgaccaataa
    19561 aaacggcagc acgcccggca acacaatacc tgaatgaagg ctgcataatt cgcggaaatt
    19621 aacccgctgc attatttcag gcgaaatgtt tatgaaaaat tgtctactgt tgggcgcgct
    19681 tttaatgggc tttactggtg tggcgatggc gcaaagtgtc accgtcgatg tgccgagtgg
    19741 atacaaagtg gttgtggttc ctgattcagt cagcgttcca caagcggtca gcgtcgcgac
    19801 tgtgccgcaa accgtctatg tggctcctgc gccagctccg gcttaccgcc cgcatcctta
    19861 tgttcgccat cttgccagcg taggtgaagg gatggttatc gaacaccaga ttgacgacca
    19921 tcaccattaa ttcgagaatg cctgatcgca caaagcaatc aggcattttt ttacccttcg
    19981 tgagaatttc ccatctgttt atcttttaaa aagataacca tcagcgacag ccacaaaatt
    20041 ccgttgacaa ggttgaagag attaaactgt ccgttgcccc cgttcagcca ggcatgtttg
    20101 ctgatttcaa taccaacggt aaagatcagc atttgcagca ttcccatcgc cgcagaaacc
    20161 gtacctttac tcatatcgct ggcaaacagg gttaatcgca ccagtcccgc attcgccaga
    20221 ccaataccga aagcataaat acttaacccg gcagtcatcc ataaatacgc gtgcgatgag
    20281 ataaccgttg ccgcagcagc gaccaacaga ccaatcataa tcggccagcc gcccataatt
    20341 atcagcgaac gtacggtgcg gcgcgaggtc agacgcgcta acagcaagtt acccgcaatt
    20401 aacgccccga aaataggcac ttgcagcaag ccatattcat agctgctcaa ctgctcgccg
    20461 gtaatgatga taatcggcga ctgggcgatc cacgccagca atggcaggct aacgaatccc
    20521 agcgccagcg cccccgccac aaagcggccg ttcttcagca ccagcttata gtcacgaccg
    20581 agttctttca gtgacagttt ctcgcctata cgcgtggcgg tttcaggcat ggctcgttgc
    20641 agaccgaaaa aggagatcgc tgccaatgcg gcaaacaaaa caaacatccc ctcccagggc
    20701 agcacatgga tccacgccgc gcccaccagc ggaccaagta gcggagcaat cagcgccacg
    20761 ttcgccatca gcgcggtgat cttgatacaa accgcctctt cgaaggattc ctgaattgcg
    20821 gcgtatccca cagcgccaat gaaacagagg cttatgccct gcaagaagcg caacagggtg
    20881 aattgttcaa tgttttgcgc cagtaatatt gccagacagg tgacaataaa ccacaccaca
    20941 cccgccagca tcaccggacg gcgaccaata cgatccgaca acggccccag gagccattgt
    21001 aaaaacatcc cgcccgccag atacgcggtc atcgaagtag gaacccaatc aatgcccgcc
    21061 tgatattgtt ccaccacggc caacataccg ggttgaatca tatcgttgcc gatataggtt
    21121 gaaaattcgt aaagcaccag acagagaggg aaaagtaacg cctgacgtcc aagcctggca
    21181 ccggaagcta atttattttg catgcaattt cttcgccaat aataatcgcg cagagtttaa
    21241 taaaagcgca gctaacgaga aagcgaattt tgtagctgaa accacggtta agcacattct
    21301 tacattattg cgagtatagc tacgctttct ttaagtttta tttaacctat gcccgttaca
    21361 atcacccacc gtaaacaggc cgcttgaggg aaataagacg atgccgcttt acccagttta
    21421 acctgcactt tattctcaac gacttgcctg tattggctcc cttttaatca ctttgcgtcg
    21481 ggaagttatg ctggaaaatt tgaatctctc tctattctct cttattaacg cgacgccaga
    21541 ctcggctccg tggatgatct cgttggcgat ttttattgct aaagatttga ttaccgtggt
    21601 gccgctgctg gccgcggtac tttggttgtg ggggcttaca gcacaacggc aactggtgat
    21661 aaaaatcgct atcgcgctgg cggtcagcct gtttgtgtcc tggacgatgg gacatctttt
    21721 tccgcacgac cgaccgtttg tcgaaaatat cggctataac ttcctgcatc atgcggcgga
    21781 tgactcattc ccgagcgatc acggtacggt gattttcacc tttgcactgg catttttatg
    21841 ctggcatcgc ctgtggtccg gctcactttt aatggtgctg gccgtcgtca ttgcctggtc
    21901 gcgcgtttat cttggcgtcc actggccgct ggatatgctc ggtggattgc tggcaggtat
    21961 gattggctgc cttagtgccc agattatctg gcaagcgatg gggcataaac tctatcaacg
    22021 tctgcaatcg tggtatcgcg tctgttttgc attaccgatc cgcaaaggct gggtgcgtga
    22081 ctgatctctg ctaaaaagtg tagtattgag cggctcgctt caataactat tcagagggat
    22141 tatggaaaca cgtcgcgaag agcgtatcgg gcagctgctg caagaattaa aacgcagcga
    22201 taagttacat cttaaagacg ccgccgcgct gcttggggtt tcggagatga cgattcgtcg
    22261 cgatctgaac aaccacagtg cgcccgtcgt tttgctcggc ggctatattg ttctggaacc
    22321 gcgcagtgcc agccattacc tgttaagcga tcaaaaatcc cgcctggtgg aagaaaaacg
    22381 ccgggcggca aaactggctt cgacgctggt agaacccgat cagaccctct tttttgactg
    22441 tggcaccacc acgccgtgga ttattgaagc gattgataat gaaatccctt tcaccgccgt
    22501 ttgttattcg ctaaatacct ttctggcgct gaaagagaaa ccccattgcc gcgcatttct
    22561 gtgcggtggt gaatttcacg ccagcaacgc cattttcaaa cccatcgatt ttcagcaaac
    22621 gctgaataat ttttgcccgg atatcgcttt ttattctgcg gcgggcgtgc atgtcagtaa
    22681 aggcgctacc tgttttaatc ttgaagagtt gccggtaaaa cactgggcca tgtcgatggc
    22741 gcaaaagcat gtgctggttg tcgaccacag taaatttggc aatgtgcgtc cggcgcgcat
    22801 gggtgacctg aaacgctttg atattgtggt gagtgattgt tgcccggaag atgagtatgt
    22861 gaagtacgcg cagacgcacc gtattaagtt gatgtattaa tgacgtataa ccggatgacg
    22921 tttcgcgcca tccggttatc agaagattaa gagaaccagc tgccgaacca ctgatggaat
    22981 ttcatcatca cgaaatccca catccgacca aagaatccgc cctcttccac attttccatc
    23041 acgatcagcg gacgctgctc aatggattta ccgttaagct ggaaatcaat ggtcccgaca
    23101 acctgacctt ttttcagcgg tgcggtaagc tgcggttccg ttaacgtgta gctcgccttc
    23161 aggtttttca gctgcccacg cggtatggtc acggagcccg cttcgcctgc cccgagattc
    23221 acttcgctct tatcaccaaa ccagacgcgc tgagtcacaa aggtggcatc aggtttaatt
    23281 ggcgtcacag tttcaaagaa gcggaaaccc caggtcaata atttctcaga ctcattaaaa
    23341 cggatacggt cagttttcgc ccccagcact acggagatta aacgcatatc accctgggta
    23401 gccgaagcaa ccagattata tcccgcacct gccgtggttc ctgtcttcat gccatcaaca
    23461 ttcagattgc tgctccacag cagacggtta cggttaggct gacgaatttt gttgaaggtg
    23521 aactctttct ctttatgaat ggcgtactct tccggcacat cgtggatcaa tgctttaccc
    23581 agcaatgcca tatcgcgcgc ggtactgaac tgccccggcg catccagacc gtgcaccgtc
    23641 tggaaggtag tgttggtcag acccagtttt ttggcataac cattcatcaa accaataaat
    23701 gactcctggc tcccggcaac gtaatcggcc agcgcaatac aggcgtcatt accggactgg
    23761 ataatcacac ctttgttcaa gtctgccacc gaaacctgat cgcccggttt gaggaacatt
    23821 accgatgaac cacgcagtgc cggatttccc gtcgcccagg catctttacc gaccgtcacc
    23881 atatcggtga gtttaatctt atcggcctta agcgcctgcc caaccacata gctggtcatg
    23941 attttagtca ggctcgcggg atccagtttc tcatccgcgt tgccttctgc cagcacttta
    24001 ccgctggcgt aatccattaa aatccatgca cgcgcatcca cgctcggcgc ttcaacggtt
    24061 tgttccgccg cgaatgccgt tggggcaaaa aggaataaaa atgcagaacc cgctgcaaga
    24121 ccacgaagga gagaggagta ttgcgtcata aatgccaccc gagtatccat tcaaaaaaaa
    24181 tacgttatat cgccgtatga taagttcggt gattaataac gcacacacaa acttaaagaa
    24241 accgtaagtt ggtaaagttt ttaaagttta tgcaatatcc tgccgcttcg ctgcttctgg
    24301 tgtcattttt ttgccagctg ttgcttaaaa gttaacttta cttcaccatc ggagtcctgt
    24361 tcagcctgtg gctacctgtc ggaaaagggg tgagtatgat tacgctgtgg ggtcggaata
    24421 attcaaccaa cgtaaaaaaa gtattgctga cgctcgaaga actggaacta ccttatgagc
    24481 aaattctcgc gggccgtgag tttgggataa atcacgatgc tgattttctg gcgatgaacc
    24541 ctaacgggct ggtgccgttg ttacgtgacg acgaaagtga tctcattctt tgggaatcaa
    24601 acgccattgt ccgctatctg gcggcacagt acgggcaaaa acgcctgtgg atcgactcac
    24661 cggcacgtcg tgcggaagca gaaaaatgga tggactgggc aaaccagaca ctcagtaatg
    24721 ctcatcgcgg gatcctgatg ggattagtca gaacaccacc ggaagagcgc gatcaggccg
    24781 ccattgatgc cagttgcaaa gagtgcgacg ccctgtttgc cctgctcgat gcggaactgg
    24841 caaaagtaaa atggttctct ggcgacgagt ttggtgtggg cgatatcgct atcgcaccgt
    24901 tcatctacaa tttgtttaat gttggcctga cctggacacc gcgtccaaat ctgcaacgct
    24961 ggtatcagca actcactgaa cgccccgcag tgcgcaaagt ggtgatgatt cccgttagct
    25021 aattgcgtgg gctaacttta agtaattccc cactggactc gtcggtgaga acgtataaat
    25081 aaccgtcggg tccagtgcga acatcacgaa ttcgctgccc tctgtccgtt aaaatacggc
    25141 catcttctgt cactttgtcg ccgttgacgc tcatcacaat gacatcttta tctttcagcg
    25201 cgccaataaa taatttttgc tgccactggg ggaatttatc gctgttatag aaggccatgc
    25261 cgctcacggc gggcgaatct ttccagtaaa aaacaggttg ctcggtcccg gcgacgatct
    25321 cccctttcgc ttccggtatc ttaaagcctg aatagttgat tccccaggtt gccagcggcc
    25381 agccgtagtt tttgcctttt tgcgggatat taatttcatc accaccgcgc gggccatgtt
    25441 cattcagcca cagtgcatta ctccacggat tcatcgccat tccttgcgga ttacgaatgc
    25501 cataagacca gatctcggcg cgcgcaccgg attcctttat aaaaggatta tcatccggga
    25561 tttcgccctg gtcggtcaga cgcaccagtt tgccctgtaa tttatccaga tcctgcgccg
    25621 tcgggcgctg attgttttcg cccagagcaa taaaaagata acctttaccg tcgaatacca
    25681 gccgcccgcc aaaatggttg ccggtagaca gttttggcat ctggcgaaag acggtgcgga
    25741 agtcggtcac ttttgagaga tcatcactta agcggccata acccacagca gttccggctt
    25801 tgccatcatc gccaacttcg gaataactta accagatgcg gcgagactga gcaaaatcag
    25861 gcgctaaaac cacgtccagc aggccgccct gcccgtgcgc ccaaacgtcc ggcactccgg
    25921 aaagcggcgc agataatcct tttcctgctt gccagtgacg caactcgccg ccgcgcagag
    25981 tgattaacat accgtgatta tcgggtaaaa aggccagtga ccagggatgg tcgagtttgt
    26041 cttgcagtac ttcgacattt accgttgcag gagccgccca gagagcggaa gaaagacaaa
    26101 taaggggcac aaggaaaaag aatggtcgat gcataaggcc tccggattga ctctggctta
    26161 aagcgtagtc agtggaggag ataacaaatt catttttaca aaaacttaaa catgaagggg
    26221 gagacgcttt ctccccctta gttttcaggc cttctcaagc atggcgtgct tctgcaggct
    26281 ctggatactc agcgttaagc tcatcagaca attttcaagc ttatcggcgt tgacggtaat
    26341 aacagtcggg caatcatggt gcccactcat caaacatact gcggctgtcg ctaatgcttc
    26401 ttcagcatga tgaagagcac tccactcttc ctgatccaga tgaagattca accgcagcga
    26461 tttatcgtgc agttcgcgat tcagtttaaa aaagttatct cgtagatgat tgctttcgct
    26521 gacggacatg tatccttttg ccttcctcag ttgcacgtat gcagcgaggt caacgcgtgc
    26581 gtcgatcaac cggttcagca gatcgattcg ctgtctgtca acgaacatac ttcgatcctc
    26641 ctctttccag ccttgttgca attaaatagt atggctattt tttgagcaaa atgctgtcca
    26701 ggatcaattc ttcacgccga attaacaaaa tttacatttc cttcgcctcc gccagcgaat
    26761 gagattgcga tacaaatttt atccagaact ctgtagaatc cctgccctga cctttcctgt
    26821 aactgaatat tttttaagct atgagcaaag taactcccca gccgaaaatc ggctttgttt
    26881 cccttggctg tccgaaaaac cttgtcgatt cagagcgtat tctcaccgaa ctccgcactg
    26941 aaggttatga cgtggtaccg agctatgacg atgcggacat ggtgatcgtc aacacctgcg
    27001 gctttattga cagcgcggta caagaatcac tggaagccat tggtgaagcg ttgaatgaaa
    27061 acggcaaggt aattgtgacc ggttgtctgg gggcaaaaga agatcagatc cgcgaagtcc
    27121 acccgaaagt gctggaaatc accgggcctc atagctatga gcaggttctg gagcacgttc
    27181 atcactacgt gccaaaaccg aaacacaacc cattcctgag cctggtgcca gaacaaggtg
    27241 tcaaactgac gccgcgtcat tatgcctatc tgaaaatttc tgaaggctgt aatcaccgct
    27301 gcaccttctg cattattccg tctatgcgcg gcgacctggt gagccgtccg attggcgaag
    27361 tgttaagtga agcgaaacgt ctggtagatg cgggcgttaa agagattctg gtgatctcgc
    27421 aggatacttc cgcctatggc gttgatgtta aacatcgtac tggcttccac aacggcgagc
    27481 cggtaaaaac cagcatggtc agcctgtgcg aacagttatc gaaactgggg atctggacac
    27541 gtctgcacta cgtttaccct tatccgcatg tggacgacgt catcccactg atggcagaag
    27601 gcaaaatcct gccgtatctg gacattccgt tgcagcacgc cagcccgcgc attctcaaac
    27661 tgatgaagcg tccgggttct gtagatcgcc aactggcgcg catcaaacag tggcgcgaaa
    27721 tctgcccgga actgacccta cgctcaacct ttattgtcgg cttccctggc gagacggaag
    27781 aagatttcca gatgctactc gacttcctga aagaagcgcg tctggatcgc gttggctgct
    27841 ttaaatacag cccggttgaa ggtgcagacg ccaatgccct gcctgaccag gttccggaag
    27901 aagtgaaaga agaacgctgg aaccgtttca tgcagctgca gcagcagatt tccgccgagc
    27961 gcctgcaaga gaaagtgggc cgtgaaattc tggtgattat cgacgaagtg gacgaagaag
    28021 gcgcgattgg tcgcagcatg gcagatgcac cggaaatcga cggcgcggtt tatctcaatg
    28081 gtgaaaccaa cgttaagccg ggtgatatcc tgcgtgtgaa agtcgagcac gccgatgagt
    28141 acgatttgtg gggtagccgg gtttaattaa ttcaggccgg gcaaaatcat ttgtccggcc
    28201 tgcgaataat gattactgcc agctaatctg tgccctgccg tttttcttac tcttatacag
    28261 ccgctcatcc gccagcattt ttaagcgttc gacggtagta cattcatttg aatgcgctac
    28321 accgatactg gtgcttaaat taatgcttcc cacctggaaa tgaacgattt cttttagaat
    28381 cgatagcaac tcttcctgcg ttccacccgt ataaatcacc gcaaattcgt caccgccgaa
    28441 gcggtagact ttacctttat ctaccgggat ctgtgattta aggattttca ccactttcat
    28501 caacacttca tcgcccagca aatgaccgaa ggtgtcgtta atgtttttga atttatcgac
    28561 atcaaacagg cacaaataac cgcctttttc cggcagagct tttaaatctt cgtcaaagga
    28621 ttttctgccc attgctttgg tcaggtcgtc caggaacgcc gagttataca tactgtagag
    28681 ttttttgaca tcggcagccg aaaagaaaag cccgccatcc agtacgcgtt caaaatggtt
    28741 attaatgatg gcttctttct ccacaatacc acgattaatc actctgcgta ccagatagag
    28801 gcaactggca cccgatatca ctgccaccaa tgcggcaaag aaaataatca atgtacaaac
    28861 ccgcaaataa acatcttttt tatacgcaaa tacgtacaat ccggtcggca aataggcaaa
    28921 gtgctccatc gcttccgaaa cggtgaagat ggcgttttta gcattatatt catcaccggt
    28981 caccggttta ccgttcttaa atagagagaa catcaggttc tcgctatcca tcgccagcga
    29041 ttttgacagt aaagaaaaac gctccagacc aagcaaaaat atcagactac gattgtgagt
    29101 taccggatag aggtaataga cgaaggtttt accgtcaaaa gagataaacc ctgtattttt
    29161 gacatgtcga tacttcacaa cctgcataat atctgcacaa taatttttct cgaactcctt
    29221 tacctgaata cattcgtcac tggaaatcag ataaggaccg acatcgtaca aataagaggg
    29281 tgaaaccgag tcggcgtaag ctgtgcgtaa gccaaccaac agctgttcct ccgtgggatt
    29341 tagcgtgttg ataagatcaa ttgccacgct acgccgatat tcagcaatat ttgccaccgc
    29401 aaaagcagta tcaataacgg actgtttcag gctatacacc cgttccttta ccatttgagt
    29461 gtagatcccg caggcaactg ctgaaatcat gataaaaatc agcagactga ctgtaagtac
    29521 gaacttattg attctggaca tacgtaaact actcttttac taattttcca cttttatccc
    29581 aggcggagaa tggcatgggt ttctggtaca gatacccctg agcctgaaca catcctaatt
    29641 tgattaattc gcgcgcaacg tcttcgctct ctacgccttc cgcaatcacc gggatccggt
    29701 tatagcgtgc caggtcgaaa ataatgcgca caatttttcc cgcagtaccg aaaatatcgc
    29761 cggtaaattg cgagtccacc tttatgctgt ctggattaat ttcataaaaa cgtttcaggt
    29821 ttgacaaacc ggtgccaaag tcatcaatcg atatccgtac cccttgttta cgcagttgtt
    29881 caatattgag ataaaaatgt tcattaattc gctcgatcac ggtttcagtg atctcaacac
    29941 agaggctgtt cgccttcagg ccattttctt tcatgatggt ggtaaaacgc tcatgaaagt
    30001 gcggttcgtt taactgccag ggcgaaacat taatatgcag taagaaatca tccttcagcg
    30061 atgcacgctg actaaaatgt ctgaattcac ccattgccgt tttaataatc tgataaccca
    30121 gctcattgat aaacccgata tcttcagcaa tggtaatgaa cttcagcggt gaaatgatcc
    30181 cccgctccgc agacacccaa cggcacaacg cctccgcgcc cagaatggcg cgggtgttaa
    30241 tatcaacgat cggttgatag acgaggaata gttcaccaat ttccattgca tggcgaatat
    30301 tacgcgcaat ccaaatattc tcttctattt cgttgagttt cgcgtcatca cagaaaataa
    30361 actcggagaa catatggtca cggatactgg aaatcgccag tttcagtttt tctgcgtaac
    30421 tccaggcgtt ttccccctgc aaaggttcac aaatggcaga cttatggatg cgtagattgc
    30481 ggtagccaaa gctgctgtca aaatcgttta ccatcgctat tttacgggta aatgtctgta
    30541 cattttcccg acacagaagt aagtagagat cggcattgaa tgtcacgata tcgcagcatt
    30601 gtgcatattt ctcggcaatt cgctgacaca atttcctgac gattttattg cgttcatcaa
    30661 caccaaacac cgcttccacg gcttccagac cacacagttt gatgatcccc acagccagat
    30721 actgattatt gttggactca ataatcgcca tatttttaat aaaaccgctt tcctgatcgt
    30781 aagagatcag tgagagcaat ttatttgacc gttcaatttc ggcatctttc atttccgcga
    30841 tcgaattttt gatgctgata atctctttcg catcgccttc gcgaattttc agagcctcga
    30901 tctgatccgt tgagtcgggc aaaaacgcca gcgtattaac cagctcggtc atttcagaaa
    30961 attgccgacg gaacacacga gtggtcatga aatataccac cagcaacacg ataataatga
    31021 acgcaaacgg tagataaaaa agtttatctg atgcgttcaa taaattaccg ccaggataat
    31081 aaaaatagat ttcgaacaat gcattatttg gcactgctga tgtctttgca atatactccg
    31141 tatcgacaga atcaataata ttataattac cttcgctatt ataaataatc agtttttcac
    31201 gagcgagttg attcttggca atgatctcat gaccttgttt caaaaataga acttcatcat
    31261 tagcgctgat ttgatcaaga tactcgttca gataccgcca gtttaaatcg acatgaattt
    31321 tatctaccgc taacaaatcg tgatctttgc cgttgatacg tagcgtaaaa gagatacatt
    31381 ttttacccgg acgatcaaag caattgtatg ccggattcca gctgttgtta gagtcatctg
    31441 aagccaccga ccacggtcgt ttcgatgggt taaaatcggc aaaaataacc cgccctgact
    31501 cgtcgacaag cggtccattt acttgttcct gatgataaac aacgatttta tcattcgccc
    31561 tccgtgaaaa gagataatat ttattatcat caagtaccag acccaccgag tctaaaaatg
    31621 gcatcatttc cattttcaga atcacggcgc gttgaatctc ctctgtattt tttaataaat
    31681 acagtgagtc ctttaatgtc agggccgaac gttcaatatc tttaaagaaa tagccaacgt
    31741 ttttctgaat aagttcaaag cggctatcgg cacgttttac cgcgcgttcc gataccagtt
    31801 gatagttaat gatgaaaaaa agaccaataa atgacagtgc tgccaataaa aataaaatta
    31861 tcagccttat ctttatcttt tcgtataaac tcagcatgtt aatacattta acaaaataac
    31921 tatctgaacg ctgtgccgct gcaaaaatag tcccttgctg cggcttttta accaaattac
    31981 ccgattctac aaaatcagca gaaagttaca acacttgctt agggcaataa tttcctgtga
    32041 ggtgattacc ctttcaagca atatttaaac gtaactatcc tttaattttc ggatccagcg
    32101 catcgcgtaa accatcgccc aacaaattga acgccagtac ggtcagaaaa atagccaggg
    32161 ccggaaaaac agcgacatgc ggcgcgataa ccatatccgc tcgtgcctca ttgagcattg
    32221 ctccccactc tggtgtcggc ggctgcgcac cgaggccgag aaatgacagg ctggcggcag
    32281 agataatcga ggtgccaatg cgcatggtga aaaacaccac gatagacgag acggtcccag
    32341 gcaagatatg acgcaataaa atggtcatat cgctggcacc aatactgcgt gctgactcaa
    32401 taaaggtttg ctgtttcaac accagcgtgt tgccgcgcac cagtcgggca aacgcgggga
    32461 tggaaaaaat ggcgactgcg ataatcacgt tagcaatgcc gcttcccaac accgcaacaa
    32521 cagcgatcgc cagtaaaata cccgggaagg caaacagcac atcgcaaatg cgcatgatca
    32581 gccgatccca ccagccttca taatatccag ccaacaagcc cagcaacgtc cctatcgccg
    32641 caccgataaa cacggcaaac acgcccgccg ccagcgagat ttgcgcacca accaggacac
    32701 ggctgaaaat gtcgcgcccc agtgaatcga cgccaaacca gtgctgcaaa gaaggtccat
    32761 tattcagatt gtcataatca aaataatttt cggcgtcata gggagcgatc cagcgtgcaa
    32821 aaatggccac cacaatcaat aaaataacga ataatgcggc ggtcatcgcc atatgctgac
    32881 ggcgaaatcg tcgccagaat tcatgccacg gtgtacgcac ctggtcaggt ttgaccagtg
    32941 gcatggcgtt taacaccgcc tgacgtcgcc agttaaatag tcgcatcctt acttgtacct
    33001 gatagccggg ttaatggcgg cgtaaagcac atccaccact aagttgataa gaataaattc
    33061 cagcgagaaa agcagaattt ccgcctgaat caccgggtaa tcgcgcattt ctacggagtc
    33121 aacgagtaag cgtccaagtc cgggccagtt gaaaactttc tccaccacga tagaaccacc
    33181 gagcaaaaag ccaaactgta agcccatcat ggtcactacc gggatcatcg cgttacgtag
    33241 cccgtgtttg aggacaaccc aggtttcgct cacccctttc gccctcgcgg tacgcatata
    33301 atcttcgctt aaaacatcga caaacgacgc gcgggtaaag cgcgccatca cggctgccac
    33361 tgccgcgccg agcgtcaggg agggtaaaat gtagtgctgc caactgtctg ctcccacggt
    33421 aggcagccag cccagttcaa cggagaatac ctgaattaaa agcatcccca gcgcaaatgc
    33481 cggaaacgag atccccgaca ccgcaatggt catactcaat cgatccggcc aacgattacg
    33541 ccagacggcg gcgataattc ccgccgccat accaaatatc accgcccaga ccatactggt
    33601 tatggtcagc cacagcgttg gcataaagcg gctggcaatc tcatcggcaa ccggacgacg
    33661 cgacaccatc gacaggccaa aatccccctg cacagcattg ctgatatagt gccagaactg
    33721 gtgatacagc ggctgatcca accccagctg ctgacgcacc agttctataa cctgcgcatc
    33781 agcttcgggc ccggcaatca atcgcgccgg atcgccgggc agcatatgga caaataaaaa
    33841 caccagcacc gagacgataa acagcgtcgg aatcaacccc agtaatcgtt tgataacgta
    33901 attaagcatt ccactccctg cgttgcttat tgcaaatccg cgtcttcaaa gctgaagccg
    33961 gtgtctggca tgatccaaaa accggtcagg tttttactgt gtgccgacac cagtttttct
    34021 accaccagcg ggatccacgg cgactcttgc cagatgatat cctgcgccgc cttatacaag
    34081 cgggtctttt ccgccggatc attagttttc agtgcctgag ccaggaagtt atccacctgt
    34141 ttattgctgt aaaacgcggt attaaacagc gtcggtggcc agttttgcga tgcaaacaac
    34201 ggcgatagtg cccagtccgc ttcgccggtt gaagccgacc agccagtgta gaacatccgc
    34261 acgccgctct ctttttgccc tttaccttca acttctgccg cccgctgtcc ggcatccatc
    34321 gcagtcacct gggctttaat cccgacctgt gctaactgct gctgggtaaa ttgcagcact
    34381 ttctgcgcgg tgctgtggtt atgtgacgac cacagcgtgg tactgaaacc gttgggatat
    34441 cccgcctctt tcagtaattc gcgcgctttc actggatcgt aaggccacgg tttataactt
    34501 tgcgcgtagg cgatactcgg cggtaccaca ccagtagctg gcgttgcata gcccgcgaag
    34561 gctactttca ccagcgccgg gcggttaatg gcgtaattca gcgcctcacg gaccttcggg
    34621 ttatcgaatg gcttttgcgt cacgttcata ctgatataac gctgcataat tgacggactg
    34681 gccatcaact cgatattttt gtttttctcc agcagtgcgg cctgctcgta aggaatgggg
    34741 aaagcaaact gcgcttcacc ggtttgcagc attgccgcgc gggtgttgtt atccgccacc
    34801 ggacgccagg ttatgctgtc cagtttgggc aatcctggct gccagtaacc cgcgaatttt
    34861 ttcaccttca caaaatcggt ctgattccag gtatccagtt catacggtcc ggttcccacc
    34921 ggatgaaaac caatctcctt gccatagttt tccagcgctg ccggtgaaat catcgcggtc
    34981 gccggatggg caagaatatt aataaacgct gagaacggct gtttgagggt aatctttacc
    35041 gttgtcggat cgatcgcttc cgttttagca atattcttat acaggttata gcgtttaaga
    35101 tgattcgccg gatcgctggc ccggtccaga ttcgctttta ccgccgctgc gttgaaatcg
    35161 gtgccatcct ggaatttaat gccttcccgc aatttcacag tgtaagtaat gccgtcatcg
    35221 gaaacggtat aactctccgc cagcacgttt ttcagtttca tctctttatc cagaccgaac
    35281 agcccctggt aaaacgattt cgctacggcc tgagataacg tgtcattcgc gtcatacgga
    35341 tcgagcgtgg tgaaattcga tcctaccgcc accaccacat ctttcgcagc gaatgcacaa
    35401 gatgccatca acgctgtcgc aatgcccagc gccactaacc cactacggtg tacagctctt
    35461 gccattttat tctccgcctg aatgttatct acgcatgaat gcgtattctg attgtggttg
    35521 tgcgacgtaa tgccccggcc cgacgcattg caacgagacg gctgccacct cttcgccacg
    35581 cagatgaata ttgctgggaa gatcgtccga cagcagtaca cgctgcggtc gttgtcggga
    35641 cggttcagcg accggaactg ccgccagtaa tttacgcgta taaggatgct gcgggttttc
    35701 gaagaccgcg cgccgtggac caatttcaac aatttgcccg agatacatca ccgccacacg
    35761 atgactaatc cgctctacca cagccatatc gtgggagata aacagatacg caatgccgaa
    35821 atcacgctgg agatcgagca acaagttgat aatctgcccg cgaattgaaa catccagcgc
    35881 cgaaacggct tcgtcggcaa tgatcacttt tggattcaat gccaacgcgc gagcaatgca
    35941 gatgcgctgg cgctgaccgc cggaaaactc atgcgggtaa cgccaggcat gttcaggtaa
    36001 caggcccacg cgctccagca accacgcaac gcgtgcagcc gcgtctttac ctggcaataa
    36061 accgtgtacg cgcagcggtt cgataatcga atcaccgatg gtctgacgtg ggtccagcga
    36121 agcgtaaggg tcctgaaaaa taaactgaat atcccggcgt aatgcctgaa gtttgccggg
    36181 tgacaaggta tcgattcgct gaccgttaaa gataatttcg ccgccctgcg attcgaccag
    36241 gcgcagcaac gcccgcccgg tagtggattt accactgcca gactcgccca ccagcgatag
    36301 cgtttcgcca ggccagagat caaaactgac tttctcaacg gcatgcactt cccgcgttac
    36361 gcgattcaac aaaccgctgc gcaaagggaa acgggtgacc agattacgca cccgtaaaac
    36421 aggttcgcca tccaccaccg ttttctgctc gatggggggg gcctgtttcg ctggatgttc
    36481 aagcgatatc aatgggaaac gtcggggata atctaaccct ttcatcgcac caagttgcgg
    36541 aacagcagct aacagcgcac gggtgtaagg atgttgcggt gcatgaaaaa tctgttcgac
    36601 ggtacccgtt tccaccgcct cgccctgata catcaccagt acccgatcgg caatctctgc
    36661 caccacgccc atatcgtgag tgataaagat aacgcccatc gacatctctt tttgcaatac
    36721 tttgattaat tgcaggatct gcgcctgaat agtgacatcc agcgcggtgg ttggctcatc
    36781 ggcaatcagc accgccgggc ggcatgacag cgccatcgca atcatcactc gctggcgcat
    36841 cccgccagag agttgatgcg gataacgtga aagaatggtt tgtgcctccg gaatgcgtac
    36901 ctgatccagc atccgcttcg cctcgaccat cgcttcttca cgactggcgt tttgatgcag
    36961 acgaattgat tcggcaatct gttcacccac agtaaatact gggttcagcg atgtcatcgg
    37021 ctcctgaaat atcatcgcca tatccgcacc gcgcacatga cgcatttgtg cagcgctctg
    37081 ctcgctaagt tcaatcactt cacgactgcg ccgccgcaac agcattttat cgcactgtac
    37141 taaaccgccc gcctgttcca acagacgcat caatgccagc gcagtcactg acttaccgga
    37201 gccggattcg ccaacaattg ccagcgtctc accgcgttgc aggctaaaag agagattgcg
    37261 gaccgcagct attttctgct ggtcctgcat aaaggcaata ttaagatttt caaccgccag
    37321 cacattaccg gcatcaagtt catcactgtg tggcaacggc gtcccctttt tcacggtaga
    37381 taccggtggt tggcgtatcg cctgcgtagc cccaggcgcg atacattcct tcggtgttaa
    37441 acggtagcgc gacattccct tcatggtcga tagcgattaa gccaccgcta ccgccaagcg
    37501 cagggagttt ttccattact acccgctcgc aggcttccgc gagacttaat ccgccgtaat
    37561 ccattaacgc ggcgatgtca tatgccgcca gcgcgcggat gaagacttcg cccgtgccgg
    37621 tacaagaaac cgccacactg gcgttattgg cgtagcatcc ggcacccact aaggggctat
    37681 cgccaactcg tccgggtaat ttattggtca ttccacccgt ggacgtggct gccgccagat
    37741 tgccgtctaa atccaacgcc acggccccca cggtgcccat tttctgtttt tcatccagtg
    37801 gcgcaccgct atggtcgagg actgttgccc cttcctcgcg cgctgccatt agttgttcat
    37861 aacgcaaagg cgtggagaaa atctccggcg agacgcgctc catgccacga gtaaacgcaa
    37921 aattttctgc cccttcgcca atcatcatca catgcgggct ttgctccatc accagccggg
    37981 cggcaagaac cggattacgc agatgactaa cgcccgccac cgcaccggct ttcagggtgt
    38041 taccatccat cacacaggcg tccagttcat gggtttcatc acgcgtaaag acagcgccaa
    38101 ttccggcgtt aaacagtgga cactcttcca gcagacgcac cgtttccgtc accacatcca
    38161 gcgcactttc gcccgcttcc agcattttct gcccggtttc aacaatggca gacaacgcct
    38221 cgatgtagcg taattcctgt tgcagactca tctgcgcgcg gctaattgca cctgcgccac
    38281 catgaattgc aatgactgct ttgcccatag tattcgtcca ttaagggtgg ctcgccactt
    38341 ttgttataaa tatcagaatt tttatgaatt actcattcaa tttttgaata tagaaagatg
    38401 gatatggcat gtaaaggcag gacccagcgc tacgcccgag gtggcataca gtctgtggcg
    38461 tttttctgac ataataggca aattcgattt tgcctccgca ggagtgtttt catggaattt
    38521 accaccggat tgatgtcgct cgacaccgcg cttaatgaga tgctttctcg cgtcacccca
    38581 ctgaccgccc aggaaacgct gccactggta cagtgttttg gtcgtattct ggcgagcgat
    38641 gtcgtttcgc cgcttgatgt tccggggttt gataactccg caatggacgg ctacgcggtg
    38701 cgtttagccg atattgcctc cgggcaaccg ctgcccgttg ccggtaaatc ctttgccggt
    38761 cagccgtatc atggtgaatg gcctgcgggc acctgcattc gtattatgac cggtgcgccg
    38821 gtgccggaag gctgcgaagc ggtggtgatg caggagcaga ctgaacaaac ggacaatggc
    38881 gtgcgtttta ctgctgaagt gcgtagcggg caaaatattc gccgtcgcgg tgaagatatc
    38941 tctgcgggcg cggttgtttt cccggcaggg actcgcctga ctaccgcaga gctgccagta
    39001 attgcttcac tggggattgc cgaagttccg gtgattcgta aagtgcgtgt agcgcttttt
    39061 tctaccggtg atgaactcca gttgccaggc cagccgctgg gcgacggcca aatctacgat
    39121 accaaccgtc tcgccgtaca cctgatgtta gaacagctgg gttgcgaggt aatcaactta
    39181 gggattatcc gcgacgatcc ccatgccctg cgcgccgcat ttattgaagc cgacagccag
    39241 gcggatgtgg tgatcagttc cggcggtgtt tcagtgggtg aagcggatta caccaaaacg
    39301 attcttgaag agctggggga gatcgccttc tggaagctgg cgattaaacc aggtaaaccg
    39361 ttcgcgttcg gtaaactcag caatagctgg ttctgcggcc tgccgggcaa cccggtttca
    39421 gcgacgctga ccttctatca actggtacag cctttgctgg caaaactaag cggcaacacc
    39481 gccagcggcc tgcccgcgcg ccagcgcgta cgcacagcgt cccgcctgaa aaaaacgcca
    39541 ggacgtcttg atttccagcg cggcgtgctg caacgcaacg ccgatggcga actggaagtg
    39601 acgaccaccg gacatcaggg ttcacatata ttcagctcct ttagcctcag caactgcttt
    39661 atcgtgctgg aacgcgatcg cggcaatgtg gaagtgggcg aatgggtgga agtagaaccg
    39721 tttaacgcgt tgttcggagg cctgtaatgg cggaactcag cgatcaggag atgctgcgct
    39781 acaaccggca aatcattctg cgcggttttg attttgacgg tcaggaggcg ctaaaagatt
    39841 ctcgtgtgct ggtagtcggc ctcggtggac tcggctgcgc agcttcgcag tatctggcaa
    39901 gcgccggtgt cggcaacctg acgctgctcg acttcgacac ggtttcgctc tcgaatctgc
    39961 aacgccagac actgcacagt gatgccacgg tcgggcaacc gaaggtggaa tccgcccgtg
    40021 acgccctgac gcggatcaac ccacatatcg cgattacgcc agtcaatgca ctgctggatg
    40081 acgcagaact tgcagcattg attgctgaac acgatctggt gctcgactgt acggataacg
    40141 ttgcggtacg taatcaactg aacgcaggct gttttgccgc gaaggtgccg ctggtttccg
    40201 gcgcggcaat tcgtatggaa ggtcaaatca cggtcttcac ttatcaggac ggcgagcctt
    40261 gctatcgctg cctcagccgt ttgtttggcg agaacgcgtt aacctgcgta gaagcaggcg
    40321 tgatggcacc gttgatcggc gtaattggtt cgttgcaggc aatggaagcg atcaaactgc
    40381 tggcaggtta tggtaaaccg gccagcggga aaatcgtcat gtacgatgcg atgacctgtc
    40441 agtttcgcga aatgaaactg atgcgcaatc cggggtgtga ggtgtgcggg cagtaattgc
    40501 caccttcagg cctgataaga cgcgacaagc gtcgcatcag gcatttgctc cagacgccag
    40561 atgcggcgtg aacgccttat ctggcctacg gttcgggcac agatttccaa gcgcggcagg
    40621 catcggtgca ctccgccacg tcatcagcaa tttatgagga tgcagaacgt ccggttaaat
    40681 cgacgttctg ccaaacgctc cctgccagtc ctgctcaaac ttcgccacag cggcatcaac
    40741 cgccggatag ctaatcatct gttgtgccac atccagtggc agagtaattg attcacatcc
    40801 tgccagtaag cagtccagag cctgacgcgg ggttttgaaa ctcgctgcca gcactttcgc
    40861 ctgcggcgca tgcattttca ataactggtg taagtcggtc acagtctgaa tgccgctacc
    40921 gccctgagca tcaatacgat taacgtaagg cgcaacatat tctgcaccag ccagcgccga
    40981 cagcaaccct tgtgctgcgc catataccgc ggttcccagc gtcggaatcc cttccgcttt
    41041 taacatctta atagctgcca gcccctcggt ggtcaccgga actttcacca cgatatccgc
    41101 aataatagaa cgcaatttac gtgcatcatt caccatccct tcagcagtgg tagccattac
    41161 ctgggcaaac agacgcccct gaccgcccat cgcttcatga agttgcggaa gcacaacatc
    41221 cagcggtttt ttacccgcgg cgataatgct tgggttagtg gtcacacccg ccagcggaaa
    41281 aatacgtgac agcgccttca ccgcaacaac gtctgaagta tccagataca gttccataac
    41341 catcctcaaa atgtactttt tgtctgaatg tagactatca cgggaaaact ctctgaacag
    41401 ttgacgcaca tcaatctaac tttcattcga aagtatttta atgtttgaac gaaacagaga
    41461 ggcaactatg attttcaata ttcagcgcta ctcgacccat gatggccccg gtatccgcac
    41521 ggtcgtattt cttaaaggct gttcgctggg ctgccgctgg tgtcagaacc cggaaagccg
    41581 cgcccgcacg caggatctgc tgtatgacgc acgactgtgt ctggaaggct gcgagctgtg
    41641 cgctaaggcc gcgccggaag tgattgagcg cgcgctgaat ggtttgctta ttcatcggga
    41701 aaagttaacc ccggagcatc tgacggcgtt aaccgactgc tgtccgacac aggcattaac
    41761 cgtgtgtggt gaagtgaaaa gcgttgagga gatcatgacg accgttctgc gcgataaacc
    41821 gttttacgat cgcagcggcg gcggtttaac gctttcgggt ggtgaaccct ttatgcagcc
    41881 ggaaatggcg atggcgctac tgcaagccag ccacgaggcc ggcattcata ctgcggtaga
    41941 aacctgtctg catgtgccgt ggaaatatat cgccccttct ctgccctata tcgatctgtt
    42001 tcttgccgat ttaaaacacg ttgccgacgc ggcgtttaaa cagtggaccg acggtaacgc
    42061 cgccagggtg ctggataacc tgaaaaaact cgccgcagcg ggcaaaaaaa tcattatccg
    42121 cgtgccactg attcagggct ttaatgccga cgaaacctct gtaaaagcca ttaccgattt
    42181 tgccgccgac gagctgcacg ttggcgaaat tcatttcctg ccctaccaca cgctgggcat
    42241 caacaaatat cacttactta atctgcccta tgacgccccg gaaaaaccgc ttgatgcacc
    42301 agaactgctc gactttgccc agcagtatgc ctgccagaaa gggttaaccg cgaccttacg
    42361 aggataacaa ccatgaccac actgaaactg gacacgctca gcgaccgcat taaagcgcac
    42421 aaaaatgcgc tggtgcatat tgtgaaaccg ccggtttgta ccgagcgcgc gcagcactat
    42481 accgagatgt atcaacaaca tctcgataag ccgatccctg tacgtcgcgc gctggcgctg
    42541 gcacatcacc tggcgaatcg cactatctgg atcaagcacg atgagttgat catcggcaac
    42601 caggcaagcg aagttcgcgc cgcgccgatc ttcccggaat atactgtctc gtggatcgaa
    42661 aaagagattg atgatctggc agatcgtccc ggtgctggct ttgcggtgag cgaagagaac
    42721 aaacgcgttc tgcatgaagt gtgcccgtgg tggcgcggtc agacggtaca ggatcgctgc
    42781 tacggcatgt ttaccgatga gcaaaaaggt ctgctggcga ccggaatcat taaagcggaa
    42841 ggcaatatga cctccggcga tgcgcacctg gcggtgaatt tcccgctgct gctggaaaaa
    42901 gggcttgatg gtctgcgcga gaaagtagcg gaacgtcgct cgcgcatcaa tctgacggta
    42961 ctggaagatc tgcacggcga acagttcctg aaagcgattg atatcgtgct ggtggcagtc
    43021 agtgaacaca ttgaacgttt cgctgccctg gcgcgtgcaa tggccgcgac cgaaacccgc
    43081 gaaagccgtc gcgatgaact gctggcgatg gcagaaaact gcgatcttat cgcccaccag
    43141 ccgccgcaga ctttctggca ggcgctgcaa ctgtgttact tcatccagtt gattttgcag
    43201 atcgaatcta acggtcactc agtatcgttt ggtcgtatgg accagtatct ctacccgtac
    43261 tatcgccgcg acgttgaact caaccagaca ctggatcgcg aacacgccat cgaattgctg
    43321 catagctgct ggctgaaact gctggaagtg aacaaaatcc gctcaggctc acactcaaaa
    43381 gcctctgcgg gaagtcctct gtatcagaac gtcaccattg gcggacaaaa tctggttgat
    43441 ggtcaaccaa tggacgcggt gaatccactc tcttacgcga tcctcgaatc ctgcggtcgc
    43501 ctgcgttcca ctcagcctaa cctcagcgtg cgttaccacg cgggaatgag caacgatttc
    43561 ctcgacgcct gcgtacaggt gatccgttgc ggcttcggga tgccggcgtt caacaacgac
    43621 gaaatcgtca tcccggaatt tattaaactc ggtattgaac cgcaggacgc ttacgactat
    43681 gcagcgattg gttgtatcga aaccgccgtc ggtggcaaat ggggctatcg ctgtaccggc
    43741 atgagcttta tcaacttcgc ccgcgtgatg ctggcggcgc tggaaggcgg gcgtgatgcc
    43801 accagcggca aagtgttcct gccacaagaa aaagcgttgt cggcaggtaa cttcaacaac
    43861 ttcgatgaag tgatggacgc gtgggatacg caaatccgtt actacacgcg taaatcaatc
    43921 gaaatcgaat atgtcgtcga caccatgctg gaagagaacg tgcacgatat tctctgctcg
    43981 gcgctggtgg atgactgtat tgagcgagcg aaaagtatca agcaaggcgg cgcgaaatat
    44041 gactgggttt ctggcctgca ggttggcatt gccaacctcg gcaacagcct ggcggcagtg
    44101 aagaaactgg tgtttgaaca gggcgcgatt ggtcagcaac agctggctgc cgcactggcg
    44161 gatgacttcg acggcctgac tcatgagcaa ctgcgtcagc ggctgattaa cggtgcgccg
    44221 aagtacggca acgacgatga tactgtcgat acgctgctgg ctcgcgctta tcagacctat
    44281 atcgacgaac tgaaacagta ccataacccg cgctacggtc gtggtccggt tggcggcaac
    44341 tattacgcgg gtacgtcgtc tatctccgct aacgtaccgt ttggcgcgca gactatggca
    44401 acgccggacg gacgtaaagc gcataccccg ctggcagaag gcgcaagccc ggcttccggt
    44461 actgaccatc tcggcccaac ggcagtcatt ggatcggtgg gtaaactgcc tacagcagcg
    44521 attctcggcg gcgtgttgct caaccagaaa ctgaatccgg cgacgctgga gaacgaatct
    44581 gacaagcaga aactgatgat cctgctccgt accttcttcg aggtgcataa aggctggcat
    44641 attcagtaca acatcgtttc ccgcgaaacg ctgctggagg cgaaaaaaca tcccgatcag
    44701 tatcgcgatc tggtagtgcg tgtcgcgggc tattccgcct tcttcaccgc gctctctcca
    44761 gacgctcaag acgatatcat cgcccgtact gaacatatgc tgtaatctcc tcggcccggc
    44821 ggcgtcgtcg ccgggctaaa tcattcacat catcaatttc atccttactt tcattcgaaa
    44881 tataatttgt gctctacgtc acattgttat tacactgtta cggtttcatt ttcgagccgg
    44941 gagcacacct atgagcgtaa aagttatcgt cacagatatg gacggtactt ttcttaacga
    45001 cgtcaaaacg tacaaccaac cacgttttat ggcgcaatat caggaactga aaaagcgcgg
    45061 cattgagttc gttgtcgcca gcggtaatca gtattaccag cttatttcgt tctttcctga
    45121 gctaaaggat gagatctctt ttgtcgcgga aaacggcgca ctggtttacg aacatggcaa
    45181 gcagttgttc cacggcgaac tgacccgaca tgaatcgcgg attgtgattg gcgagttgct
    45241 aaaagataag caactcaatt ttgtcgcctg cggtctgcaa agtgcatatg tcagcgaaaa
    45301 tgcccccgaa gcatttgtcg cactgatggc aaaacactac catcgcctga aacctgtaaa
    45361 agattatcag gagattgacg acgtactgtt caagttttca ctcaacctgc cggatgaaca
    45421 aatcccgtta gtgatcgaca aactgcacat agcgctggat ggcattatga aacccgtcac
    45481 cagtggtttt ggctttatcg acctgattat tcccggtcta cataaagcaa acggtatttc
    45541 gcggttactg aaacgctggg atctgtcacc gcaaaatgtg gtagcgattg gcgacagcgg
    45601 taacgatgcg gagatgctga aaatggcgcg ttattccttt gcgatgggca atgctgcgga
    45661 aaacattaaa caaatcgccc gttacgctac cgatgataat aatcatgaag gcgcgctgaa
    45721 tgtgattcag gctgtgctgg ataatatcgc tcctttcaat agttgatctt ccccctggcc
    45781 gaagtgacca cttcggcctc tctccttttt gctcgctttg tgcactgact caagattttt
    45841 atcttgcgtt aacttatttt taacttaatg tatcaatcgt aatcatttta ctttcgaatg
    45901 aaagaagcga ggttgatatg actttttcct gtgagacgct gcccacagat gcgaaagcgg
    45961 ctatccgaga gatgaaagct gcgctgcggg aacagctcgg tgatgtgcaa caggttttcg
    46021 atacactctc tgcaaaaatc gaaacgcgtg ttgctgaaat cgatgcccta aaagcgcaag
    46081 ggctgccggt ttggccggaa gtttcttata gtgatattgc cgcaggtaca gtaagcgatg
    46141 ccactcgcaa tgaaattaaa cgtcgcggct gtgcagtcat aaaaggccat ttccctcgcg
    46201 agctggcgat ggcgtgggat cgtgccatgc tcgattatct tgatatcaat cattttgatg
    46261 atgtttataa aggccctggc gacagttttt tcggctcgct ggaggcttct cgtcccgaaa
    46321 tctatcccat ttactggtcg caagcacaga tgcaggcgcg gcagagcgaa aacatggctg
    46381 ctgtgcaatc ttttctaaac cgactgtgga cgtctgaaag caacggcaaa cagtggttta
    46441 atcctgatat cagtgtgatt tatccagaca ggatccgccg ccgccctcca ggtactacat
    46501 ctaaaggcct gggcgcgcat accgattctg gtgcgctgga acggtggtta ttacccgctt
    46561 atcagcgcgt ttttgccaat gtgtttaatg gtaatttcga tgattacgat ccctgggatg
    46621 ctgcacaccg gaccgatgtc gaagagtaca cggtagataa cactacaaaa tgttcggtgt
    46681 tccgcacgtt tcaaggctgg acggcgctct ctgatatgtt atcgggtcag ggattgttac
    46741 atgtggtgcc tatcccggaa gcgatggcgt atgttttatt gcgcccgctg ctggatgacg
    46801 taccggacga tgaactgtgc ggggttgcac ctggcaaagt tctgcccatt tcagagaagt
    46861 ggcatccact actgcttaaa gcgctgactt ccattcccgc gataacagca ggggattccg
    46921 tctggtggca ctgcgatgtg attcactccg tcgcgccagt agacaatcaa cagggttggg
    46981 gcaacgtgat gtatatccct gctgcgccaa tgtgcgagaa aaatcgggct tatgcacaca
    47041 aagtcaaaat ggcgctggaa aaaggcgcat cacctggcga cttcccgcgg gaagattatg
    47101 aagcaaactg ggaaggccgc tttacgcttg ccgacctcaa cattcacggt aagcgagcgt
    47161 tgggcatgga tgtttgagag ttaatggaag tgaattgatg tgggattaag aaaatcgatt
    47221 cgacgttact cagattgctg acaagtgcgc tttcttaatg cctgatgcgg cgttaacgcc
    47281 ttatctggcc tacaaagtcc tgcagattca atatattaca ggtactgcgt aggcctgata
    47341 agcgtagcgc atcaggctat tttgcatttg tcatcagccg ccgtcacccc gacagtatcg
    47401 gggtgacgga taataactta ctcgatccct ttactacgca ggtaatcttc gtaattaccg
    47461 ctaaagtcga tcacgcgttc cggggtgatt tccagaatgc gggtcgccag ggagcttacg
    47521 aactcacggt cgtgtgaaac aaagatcagc gtgccctgat acagttccag cgccatgttc
    47581 agtgactcga tggattccat atccaggtgg ttggtcggtt cgtccatgat cagaatgttc
    47641 ggcttctgca tcattaactt accaaaaagc atccgccctt tttctccacc ggaaagcact
    47701 ttagctggct ttttgatgtc gtcctggctg aacagcaaac gaccgagaat actgcgtacc
    47761 gcctgctcgt catcgccttc ctgcttccac tggctcatcc attcgaacac ggtcagatca
    47821 ttttcaaact catattcgtg gtcctgagca tagtaaccaa tgcgcgcgtt ttcagaccat
    47881 tttacggtgc cgctgtccgg ttgcagatcg cccactagcg ttttcagcag cgttgattta
    47941 ccgacgccgt tggtacccag taccgcaagt ttttcaccga cctccagcag caggttgaga
    48001 tttttaaaca gtggaccgtt atcaaaccct ttggtcagac cttccacttc cagcgcgtta
    48061 cggaacagtt tcttatcctg ttcaaaacgg atgaacgggt tctgacggct ggaggctttc
    48121 acctcttcca gtttgatttt atcaatctgg cgcgcgcgcg aagttgcctg gcgagatttc
    48181 gaggcgttgg cgctaaagcg gctaacgaaa gattgcaact cagcaatctg cgctttcttc
    48241 ttggcgttat cggccagcag acgttcacgc gcctgggtcg ccgccgtcat gtactcatcg
    48301 tagttacccg gataaacgcg cagctcgccg taatccagat ccgccatgtg ggtacagacc
    48361 atgttaagga agtgacggtc gtgcgagatg atgatcatgg tgctgtcacg ctcgttcagc
    48421 acctgttcca gccagcgaat ggtgtcgatg tcgaggttgt tggtcggttc gtcgagcagg
    48481 agaatgtccg gatcagcaaa cagcgcctgc gccagcaaca cacgcagctt ccagccagga
    48541 gcaacttcac tcatcgggcc gtagtgctgt tccactggaa ttcccacgcc aagcaacaat
    48601 tcaccggcgc gagcttccgc agagtaaccg tccatttcgc cgtatttaac ttccagatcg
    48661 gccactttat agccgtcttc ttcactcatt tccggcaaag catagatgcg gtcgcgctcc
    48721 tgcttcactt cccacaactc tttatgcccc atgatcaccg tatccagcac agtgaactct
    48781 tcaaaggcaa actgatcctg acgcagttta ccaatgcgct cgttgggatc gagggaaacg
    48841 ttacccagcg tcggctcaag gtcgccgccg aggatcttca taaaggtgga tttaccacta
    48901 ccgttcgcgc caatcaggcc gtaacggttg ccgccgccaa atttgacgga aatgttttca
    48961 aacaacggct tactgccgaa ctgcatggtg acgttactgg aaactaacac aggcgtatcc
    49021 tgaaaagaga tatgacaaac cgcgcattat gccataagtc tgaaagaaga tcctgctcct
    49081 cgcttaaggt ctacactcaa gccaaaggca aaaaatgtga tttcgtacac atctgatttc
    49141 actgtgagct ggaatgaact tataatgcgc ttccaatact ctaatattct caacccaatg
    49201 gcctgccagg cacaaaatct cgcttaacat gaatatgaaa ttgaaaacat tattcgcagc
    49261 ggccttcgct gttgtcggct tttgcagtac cgcctctgcg gtaacttatc ctctgccaac
    49321 cgacgggagt cgcctggttg gtcagaatca ggtgatcacc attcctgaag gtaacactca
    49381 gccgctggag tattttgccg cggagtacca gatggggctt tccaatatga tggaagcgaa
    49441 cccgggtgtg gataccttcc tgccgaaagg cggtactgta ctgaacattc cgcagcagct
    49501 gatcctgccg gataccgttc atgaaggcat cgtcattaac agtgcagaga tgcgtctgta
    49561 ttactatccg aaagggacca acaccgttat cgtgctgccg atcggcattg gtcagttagg
    49621 caaagatacg cctatcaact ggaccaccaa agttgagcgt aagaaagcag gcccgacctg
    49681 gacgccgacc gccaaaatgc acgcagagta ccgcgctgcg ggcgaaccgc ttccggctgt
    49741 cgttccggca ggtccggata acccgatggg gctgtatgca ctctacatcg gtcgcctgta
    49801 cgctatccat ggcaccaacg ctaacttcgg tatcggtctg cgtgtaagtc atggttgtgt
    49861 gcgtctgcgt aacgaagaca tcaaattcct gttcgagaaa gtaccggtcg gtacccgcgt
    49921 acagtttatt gatgagccgg taaaagcgac caccgagcca gacggcagcc gttatattga
    49981 agtccataat ccgctgtcta ccaccgaagc ccagtttgaa ggacaggaaa ttgtgccaat
    50041 taccctgacg aagagcgtgc agacagtgac cggtcagcca gatgttgacc aggttgttct
    50101 tgatgaagcg atcaaaaatc gttccgggat gccggttcgt ctgaattaat cgtcaaaacg
    50161 taaaacaaag gcggactcat aatccgcctt ttttatttgc cagaccttac cagtccggga
    50221 tcataaccaa caaaacatat cccaccagcg acgcccacaa cagcatcggt attgaataga
    50281 gatggaaacg ccaccagatg cggcgatcgt tcgccatacg tagcgcaatc aaatttgcca
    50341 gcgatccggg taacaaccca aagccaccta cgtttaccgc ccatgccagt aataaagacg
    50401 gcggcacata gttcagcaac aatatggtac tcggcacgtt actgatcacc tgcgataaac
    50461 cgattgccgt taaccataat ccgggttcag ataaatgact cacgttaccc aacacgcctt
    50521 gcaacgctgg aagctgggtc agtaaatgga cgtcgataaa catcgccata aacaccagca
    50581 gcagcgtcca gtccacactc aacaccacgc gacgtgccag cagcgcaaag cccaccgcca
    50641 caatcaccag tccccacagc tcttgtttta actccagcgc cgtcagaaag acgaaataca
    50701 gtcccagaca actccacacc agccgcggtt tccactccgg cgtttgcacc cccgtatggt
    50761 attggagtgc ctttccaggg aaacaacacc agcacaacag caggagcgtc agcatcattg
    50821 cgccagccag cggtgccatt tgggcaataa atccggcaaa cgaaagacca gaacgtcccc
    50881 agataagaat attttgcggg ttgccaattg gcgtcagtag cgaaccagcg ttgactgcca
    50941 gcgcctcaaa aataatcagc cgattaaccg ggatctcaca cagccttttt agcgtgatag
    51001 tcagcggaac aacaataaac agcgcgacat cgttggtcag aaaggtagaa agcagcgccg
    51061 ccgccagcac cataaacatc gccagccgac gctccgtagc aaagcggcgc accattttgc
    51121 gccccagcac atcaaaataa ccgcttaact ccacaccttt ggtcagcagc atcaagccgc
    51181 ttaaggtgat gatggtgtgc cagtcgatag cagcaggcca ggatttcggt gcaaagggaa
    51241 cgaaaaaact taatccgata ccaacaagaa ttaataactg aaaaaaacga tcgccttgca
    51301 gcgtgcgtaa aaaaggcagg ctcatttggc accgtgtttc tgggtaaaca aacgaaaggc
    51361 atccagcgtc tcttcactaa catggtgctc catgccttcc gcgtcgcgac gggcgatttc
    51421 cggactgacg cccaacacca ataagaaatt ttcgactatc tgatgtcgct cgcggctttc
    51481 ctgcgccagc ttctctcctt ctgccgttaa aaacacgcct cgccagggga tcatttcaat
    51541 cagccccatg gttgctagtc gcttaagcat tttagccacc gtcggttgcg aaactcccaa
    51601 acgagcagcc atgtctacct gacgagcttc ccccacttcc ctgatcaagt cagaaatcag
    51661 ctcaacgtaa tcatcaataa gctcgcgccg atgcgcctct ctgacctggc ggaacccttc
    51721 aacgtgctct tccacgttca ctaactgcgt cacttttttt gctgttggcg tacctgcgcg
    51781 acgactcatt gtgcttcctc attatggtga cgcatacata gcgttctcat ttcgtgcgca
    51841 cattgtaaac cagagttgcg aaggtacaaa aaattaacgt tttagcaata gctatataat
    51901 atagcctgtg ctatatctgt atgtaatgca atcatccctc aaggatcgac gggattagca
    51961 agtcaggagg tcttatgaat gagttcaaga ggtgtatgcg cgtgtttagt cattctccct
    52021 ttaaagtacg gttaatgctg ctctctatgt tgtgcgatat ggtcaacaac aaaccgcagc
    52081 aagataaacc ttccgataaa tagcggcgtc gcggtacgcc gcttccctcc tgctttcatg
    52141 caggcataac gcgttttggt ctgaaaaacc ccactttttg tcggatttgc aatccccttc
    52201 gcaaaagatt tgttcgtcag tagttgacct gaacggcggc tcgctctatc ttcttgcagc
    52261 cctgcgtata ttgcggctcg cggatgcgga cccctttccc ctcttcacgc actcttgcag
    52321 gtattgaccc ttgacgccag ggtaagcaca tggcgtttgt tacgatagtg gcatatgaat
    52381 ttaaccctca aagaatcgct tgttacccgt agccgggtat ttagcccgtg gactgcgttc
    52441 tactttttac agtcgctatt aattaacctc ggcttaggtt accccttcag tttgctctac
    52501 accgctgcgt ttacggctat tttgcttttg ctatggcgaa cattgcctcg cgtacaaaaa
    52561 gttctggtcg gtgtcagttc gctggtggcg gcttgttatt tcccttttgc tcaggcctac
    52621 ggcgcgccta atttcaatac attgctggca ttgcactcca ccaatatgga agagtcgacc
    52681 gaaatcctga cgatttttcc gtggtacagc tacctggtcg gcttatttat ttttgcgctc
    52741 ggcgtaatag caatcaggcg aaaaaaagag aatgaaaaag cgcgctggaa taccttcgac
    52801 agcctgtgtc tggtattcag tgtggcgaca ttttttgttg ctcccgtgca aaacctggcc
    52861 tggggtggcg tatttaaact gaaagatact ggctatccgg tatttcgttt tgctaaggat
    52921 gtcatcgtca ataataacga ggtgattgaa gagcaagaac ggatggcaaa actttccgga
    52981 atgaaagata cctggacggt cactgccgtt aagccgaagt atcagaccta tgtggtggtg
    53041 atcggtgaaa gcgcgcgtcg cgatgccctc ggtgcctttg gcggtcactg ggacaatacc
    53101 ccgtttgcca gcagcgttaa cggtttgata tttgctgact acattgccgc cagtggctcc
    53161 acgcagaaat cgcttggctt aacgctcaat cgcgttgtcg atggcaagcc acagtttcag
    53221 gataactttg tcaccctggc aaatcgcgcg ggcttccaga cctggtggtt ttccaaccag
    53281 ggtcaaatcg gcgaatacga tactgctatc gccagcatcg ccaaacgtgc agatgaagtg
    53341 tacttcctga aagaaggtaa ttttgaagca gataaaaaca cgaaagacga agcgttactg
    53401 gatatgaccg ctcaagtgct ggcgcaagag cactcgcaac cgcagctgat tgttctgcat
    53461 ctgatgggct cgcatccgca ggcctgcgac cggacacaag gcaaatacga aacctttgtg
    53521 caatcgaaag aaacgtcgtg ctatctctat accatgacgc aaacggacga tttactgcgc
    53581 aagctgtacg atcagttacg caacagcggc agcagcttct cgctggttta cttttctgac
    53641 cacggtctgg cctttaaaga gcgcggcaaa gacgtgcaat accttgccca tgatgataaa
    53701 tatcagcaaa atttccaggt gccttttatg gtcatttcca gcgacgataa agcgcatcgt
    53761 gtgattaaag cccgccgctc agccaatgac ttcttaggct ttttctccca gtggacggga
    53821 attaaagcga aggaaattag catcaaatac ccgtttatat ctgagaagaa agccgggccg
    53881 atatacatca ccaacttcca gttacagaag gtggattaca accatctcgg aaccgatatt
    53941 ttcgacccga agccttaaaa acaaaaatcc gccccgagag gcggattttt tatatcacca
    54001 aagtgattag aagcggtaac caacaccggc aatccaggtg cctacgtcaa cgctacgaat
    54061 acggctctgc tcgtaagaga agtccagagc aacgttttcc atcgggttga actgcagacc
    54121 agcaccgtag gagaaaccgt agtcgctggt gtcgtgtttg taggtcgggt attcagtggt
    54181 ctggaattta ccataaccca cacccactac accgtagatg cttgcccagt cgttaatgcg
    54241 gtaagccgga ccagcagtga tgccgtagta ctggtttttg ttgtagtcac cagagcttgc
    54301 agtacggctt ttctcggtgt aagtgaaaga accgatcaca cccagcgggc tgttgtcttc
    54361 ttcatagcgg tatttcaggt tgaaaccgcc cattttgttc atttggccct gagcgtcgct
    54421 ctgtgcgtaa ccgccagtta cggtagaagt cgcagctacg gaagtacctg cggtgaaagc
    54481 cagaactgcg gccagtgctg aaagacatgc aatttttttc ataaccacct caaatgtgat
    54541 tcaaataagt cctaagtttt aaatatatca aaaattaatg ggaaactctt cgcgatttgt
    54601 gatgtctaac gggccatttc atgtaacaga acgtttccat acaccgctat ccatctaaat
    54661 ttaaatcact ttttcagaga actgcgtaag tattacgcat gttctccctg tcattcatcc
    54721 agattattcc taatcaccag actaatgatt ccatcaatcc tggcgcattt tagtcaaaac
    54781 gggggaaaat tttttcaaca aatgctcaac cagcattggg tatatccagt acactccacg
    54841 ctttacttaa gtctagatat ttgtgggaga aaggatgcct ggttcattac gtaaaatgcc
    54901 ggtctggtta ccaatagtca tattgctcgt tgccatggcg tctattcagg gtggagcttc
    54961 gttagctaag tcactttttc ctctggtggg cgcaccgggt gtcactgcgc tgcgtctggc
    55021 attaggaacg ctgatcctca tcgcgttctt taagccatgg cgactgcgct ttgccaaaga
    55081 gcaacggtta ccgctgttgt tttacggcgt ttcgctgggt gggatgaatt atctttttta
    55141 tctctctatt cagacggtac cgctgggtat tgcggtggcg ctggagttca ccggaccact
    55201 ggcggtggcg ctgttctctt ctcgtcgccc ggtagatttc gtctgggttg tgctggcggt
    55261 tcttggtctg tggttcctgc tacctctggg gcaagacgtt tcccatgtcg atttaaccgg
    55321 ctgtgcgctg gcactggggg ccggggcttg ttgggctatt tacattttaa gtgggcaacg
    55381 cgcaggagcg gaacatggcc ctgcgacggt ggcaattggt tcgttgattg cagcgttaat
    55441 tttcgtgcca attggagcgc ttcaggctgg tgaagcactc tggcactggt cggttattcc
    55501 attgggtctg gcggtcgcta ttctctcgac cgctctgcct tattcgctgg aaatgattgc
    55561 cctcacccgt ttgccaacac ggacatttgg tacgctgatg agcatggaac cggcgctggc
    55621 tgccgtttcc gggatgattt tcctcggaga aacactgaca cccatacagc tgctggcgct
    55681 cggcgctatc atcgccgctt caatgggttc tacgctgaca gtacgtaaag agagcaaaat
    55741 aaaagaatta gacatta
//
LOCUS       DAPGOF010000006        55260 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.006, whole genome shotgun sequence.
ACCESSION   DAPGOF010000006 DAPGOF010000000
VERSION     DAPGOF010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 55260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55260
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.006"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            20..2209
                     /gene="fhuA"
                     /locus_tag="RHV13_000300"
     CDS             20..2209
                     /gene="fhuA"
                     /locus_tag="RHV13_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HDV1239867.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMHQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTIEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIANDGHTLNRGTVVDNERLQNFSVDTQLESKFATGEVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGNAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRVSYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSAAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            2260..3057
                     /gene="fhuC"
                     /locus_tag="RHV13_000301"
     CDS             2260..3057
                     /gene="fhuC"
                     /locus_tag="RHV13_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HDV1239868.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            3057..3947
                     /gene="fhuD"
                     /locus_tag="RHV13_000302"
     CDS             3057..3947
                     /gene="fhuD"
                     /locus_tag="RHV13_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HDV1239869.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPESVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRILDNAIGGKA"
     gene            3944..5926
                     /gene="fhuB"
                     /locus_tag="RHV13_000303"
     CDS             3944..5926
                     /gene="fhuB"
                     /locus_tag="RHV13_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HDV1239870.1"
                     /translation="MSKRIALLPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLMLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(6084..7364)
                     /gene="hemL"
                     /locus_tag="RHV13_000304"
     CDS             complement(6084..7364)
                     /gene="hemL"
                     /locus_tag="RHV13_000304"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HDV1239871.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            7589..9010
                     /gene="clcA"
                     /locus_tag="RHV13_000305"
     CDS             7589..9010
                     /gene="clcA"
                     /locus_tag="RHV13_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HDV1239872.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDVFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVVPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            9034..9099
                     /locus_tag="RHV13_000306"
     CDS             9034..9099
                     /locus_tag="RHV13_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1239873.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            9092..9436
                     /gene="erpA"
                     /locus_tag="RHV13_000307"
     CDS             9092..9436
                     /gene="erpA"
                     /locus_tag="RHV13_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HDV1239874.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(9483..10106)
                     /locus_tag="RHV13_000308"
     CDS             complement(9483..10106)
                     /locus_tag="RHV13_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HDV1239875.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(10144..10944)
                     /gene="btuF"
                     /locus_tag="RHV13_000309"
     CDS             complement(10144..10944)
                     /gene="btuF"
                     /locus_tag="RHV13_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HDV1239876.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDTTSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(10937..11635)
                     /gene="mtnN"
                     /locus_tag="RHV13_000310"
     CDS             complement(10937..11635)
                     /gene="mtnN"
                     /locus_tag="RHV13_000310"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDV1239877.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            11719..13236
                     /gene="dgt"
                     /locus_tag="RHV13_000311"
     CDS             11719..13236
                     /gene="dgt"
                     /locus_tag="RHV13_000311"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HDV1239878.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRNGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIETRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            13366..14790
                     /gene="degP"
                     /locus_tag="RHV13_000312"
     CDS             13366..14790
                     /gene="degP"
                     /locus_tag="RHV13_000312"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HDV1239879.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            14945..16102
                     /gene="cdaR"
                     /locus_tag="RHV13_000313"
     CDS             14945..16102
                     /gene="cdaR"
                     /locus_tag="RHV13_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HDV1239880.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(16191..16577)
                     /locus_tag="RHV13_000314"
     CDS             complement(16191..16577)
                     /locus_tag="RHV13_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HDV1239881.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(16891..17715)
                     /gene="dapD"
                     /locus_tag="RHV13_000315"
     CDS             complement(16891..17715)
                     /gene="dapD"
                     /locus_tag="RHV13_000315"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HDV1239882.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFANYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(17746..20418)
                     /gene="glnD"
                     /locus_tag="RHV13_000316"
     CDS             complement(17746..20418)
                     /gene="glnD"
                     /locus_tag="RHV13_000316"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HDV1239883.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSPELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(20480..21274)
                     /gene="map"
                     /locus_tag="RHV13_000317"
     CDS             complement(20480..21274)
                     /gene="map"
                     /locus_tag="RHV13_000317"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDV1239884.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            21642..22367
                     /gene="rpsB"
                     /locus_tag="RHV13_000318"
     CDS             21642..22367
                     /gene="rpsB"
                     /locus_tag="RHV13_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDV1239885.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            22502..23353
                     /gene="tsf"
                     /locus_tag="RHV13_000319"
     CDS             22502..23353
                     /gene="tsf"
                     /locus_tag="RHV13_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDV1239886.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            23500..24225
                     /gene="pyrH"
                     /locus_tag="RHV13_000320"
     CDS             23500..24225
                     /gene="pyrH"
                     /locus_tag="RHV13_000320"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDV1239887.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            24517..25074
                     /gene="frr"
                     /locus_tag="RHV13_000321"
     CDS             24517..25074
                     /gene="frr"
                     /locus_tag="RHV13_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDV1239888.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            25166..26362
                     /gene="ispC"
                     /locus_tag="RHV13_000322"
     CDS             25166..26362
                     /gene="ispC"
                     /locus_tag="RHV13_000322"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HDV1239889.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            26551..27309
                     /gene="ispU"
                     /locus_tag="RHV13_000323"
                     /gene_synonym="uppS"
     CDS             26551..27309
                     /gene="ispU"
                     /locus_tag="RHV13_000323"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HDV1239890.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            27322..28179
                     /gene="cdsA"
                     /locus_tag="RHV13_000324"
     CDS             27322..28179
                     /gene="cdsA"
                     /locus_tag="RHV13_000324"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDV1239891.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            28191..29543
                     /gene="rseP"
                     /locus_tag="RHV13_000325"
     CDS             28191..29543
                     /gene="rseP"
                     /locus_tag="RHV13_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HDV1239892.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            29573..32005
                     /gene="bamA"
                     /locus_tag="RHV13_000326"
     CDS             29573..32005
                     /gene="bamA"
                     /locus_tag="RHV13_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HDV1239893.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            32127..32612
                     /gene="skp"
                     /locus_tag="RHV13_000327"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             32127..32612
                     /gene="skp"
                     /locus_tag="RHV13_000327"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HDV1239894.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            32616..33641
                     /gene="lpxD"
                     /locus_tag="RHV13_000328"
     CDS             32616..33641
                     /gene="lpxD"
                     /locus_tag="RHV13_000328"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HDV1239895.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSTALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            33746..34201
                     /gene="fabZ"
                     /locus_tag="RHV13_000329"
     CDS             33746..34201
                     /gene="fabZ"
                     /locus_tag="RHV13_000329"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDV1239896.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            34205..34993
                     /gene="lpxA"
                     /locus_tag="RHV13_000330"
     CDS             34205..34993
                     /gene="lpxA"
                     /locus_tag="RHV13_000330"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HDV1239897.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            34993..36141
                     /gene="lpxB"
                     /locus_tag="RHV13_000331"
     CDS             34993..36141
                     /gene="lpxB"
                     /locus_tag="RHV13_000331"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HDV1239898.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            36138..36734
                     /gene="rnhB"
                     /locus_tag="RHV13_000332"
     CDS             36138..36734
                     /gene="rnhB"
                     /locus_tag="RHV13_000332"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDV1239899.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            36771..40253
                     /gene="dnaE"
                     /locus_tag="RHV13_000333"
     CDS             36771..40253
                     /gene="dnaE"
                     /locus_tag="RHV13_000333"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDV1239900.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRSEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            40266..41225
                     /gene="accA"
                     /locus_tag="RHV13_000334"
     CDS             40266..41225
                     /gene="accA"
                     /locus_tag="RHV13_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HDV1239901.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            41324..43465
                     /gene="ldcC"
                     /locus_tag="RHV13_000335"
     CDS             41324..43465
                     /gene="ldcC"
                     /locus_tag="RHV13_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HDV1239902.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMSDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            43522..43911
                     /locus_tag="RHV13_000336"
     CDS             43522..43911
                     /locus_tag="RHV13_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDV1239903.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEW"
     gene            43976..45271
                     /gene="tilS"
                     /locus_tag="RHV13_000337"
     CDS             43976..45271
                     /gene="tilS"
                     /locus_tag="RHV13_000337"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDV1239904.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVAL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAETTARSAALCAE
                     QESLLDELLADDLAHCQSPQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSENIVPWQTWLQPLELPA
                     GLGSVQLNAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGISFVWQQNA"
     gene            complement(45324..45584)
                     /gene="rof"
                     /locus_tag="RHV13_000338"
     CDS             complement(45324..45584)
                     /gene="rof"
                     /locus_tag="RHV13_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HDV1239905.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(45571..45771)
                     /locus_tag="RHV13_000339"
     CDS             complement(45571..45771)
                     /locus_tag="RHV13_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HDV1239906.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            45937..46482
                     /locus_tag="RHV13_000340"
     CDS             45937..46482
                     /locus_tag="RHV13_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HDV1239907.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            46479..46901
                     /gene="arfB"
                     /locus_tag="RHV13_000341"
     CDS             46479..46901
                     /gene="arfB"
                     /locus_tag="RHV13_000341"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDV1239908.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKELTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            46915..47625
                     /gene="nlpE"
                     /locus_tag="RHV13_000342"
                     /gene_synonym="cutF"
     CDS             46915..47625
                     /gene="nlpE"
                     /locus_tag="RHV13_000342"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308221.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HDV1239909.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCVTGKRFMVANNAELERGYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGQ"
     gene            complement(47825..48649)
                     /locus_tag="RHV13_000343"
     CDS             complement(47825..48649)
                     /locus_tag="RHV13_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HDV1239910.1"
                     /translation="MDKPKAYCRLLLPCFLLLSACTVDISQPDSSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITVPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATEITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSADFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTR"
     gene            complement(48702..50420)
                     /gene="proS"
                     /locus_tag="RHV13_000344"
     CDS             complement(48702..50420)
                     /gene="proS"
                     /locus_tag="RHV13_000344"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDV1239911.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEIADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(50531..51238)
                     /gene="tsaA"
                     /locus_tag="RHV13_000345"
     CDS             complement(50531..51238)
                     /gene="tsaA"
                     /locus_tag="RHV13_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HDV1239912.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(51235..51639)
                     /gene="rcsF"
                     /locus_tag="RHV13_000346"
     CDS             complement(51235..51639)
                     /gene="rcsF"
                     /locus_tag="RHV13_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HDV1239913.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(51757..52572)
                     /gene="metQ"
                     /locus_tag="RHV13_000347"
     CDS             complement(51757..52572)
                     /gene="metQ"
                     /locus_tag="RHV13_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HDV1239914.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLAELQDGAQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(52612..53265)
                     /gene="metI"
                     /locus_tag="RHV13_000348"
     CDS             complement(52612..53265)
                     /gene="metI"
                     /locus_tag="RHV13_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HDV1239915.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(53258..54289)
                     /gene="metN"
                     /locus_tag="RHV13_000349"
     CDS             complement(53258..54289)
                     /gene="metN"
                     /locus_tag="RHV13_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HDV1239916.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            54477..55049
                     /gene="gmhB"
                     /locus_tag="RHV13_000350"
     CDS             54477..55049
                     /gene="gmhB"
                     /locus_tag="RHV13_000350"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HDV1239917.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPA"
BASE COUNT        13155 a        13597 c        15129 g        13379 t
ORIGIN      
        1 ttacatcaga gatataccaa tggcgcgttc caaaactgct cagccaaaac actcactgcg
       61 taaaatcgca gttgtagtag ccacagcggt tagcggcatg tctgtttatg cacaggcagc
      121 ggttgaaccg aaagaagaca ctatcaccgt taccgctgca cctgcgccgc aagaaagcgc
      181 atgggggcct gctgcaacta ttgcggcgaa gcactctgct actgcgacta aaacggatac
      241 accaattgaa aaaacgccgc agtctatttc ggttgtgaca aatgaagaga tgcagatgca
      301 tcaatttcag tctgtaaaag aagcattagg ttatacacct ggtgttacgg ttagcagtcg
      361 tggtgcttct aatacatatg attttgttat cattcgtggt ttctcatctg ttggtctgaa
      421 tcaaaataac taccttgatg gactaaaact tcagggcaac ttttataacg atgctgtgat
      481 tgatccttac atgctcgaga gggttgaact gatgcgtggg ccgacgtctg ttctctatgg
      541 aaaaagtaat cctggtggga ttatttctat ggtgagtaag cgtccgacca ttgaacccct
      601 gaaagaaatt cagtttaaaa tggggacgga taatctgttt cagaccggat tcgatttcag
      661 tgatgcgctg gatgataacg gtgagttctc ctaccgtttg acgggcctcg ctcgttcaac
      721 aaacgaacag cagaaaaatt ctgaatctca gcgttatacc attgctccat cattctcatg
      781 gcgtccggac gacaaaacca attttacttt cctgtcctat ttccagaatg aaccagaaac
      841 gggttattac ggttggttgc cgaaagaggg gaccgttgag ccattgccta atggtaaacg
      901 tctaccgact gacttcaatg aaggtgcatc gaataataca tactcccgta accagaaaat
      961 ggtgggatat agttttgaac atggttttaa tgacaccttc accgtgcgtc agaatctgcg
     1021 tttcagtgaa atgaaaacct cacagaaaag tgtttatggc acagggattg ccaacgatgg
     1081 tcatactcta aaccgcggga cagtggtgga taatgagcgt ctgcaaaact ttagcgttga
     1141 tacccaactt gaaagtaaat ttgctacagg tgaagtcgag catactttgc tgacaggggt
     1201 agacttcatg cgtatgcgca atgatatcaa tgccagcttt ggatctgcac catccatcga
     1261 tctttataac aaatatcatc ctgaatactt tgcatttggt aacgcagagc cataccaaat
     1321 gaatgaaagc aaacaaacag gtatttatgt tcaggatcag gcggaatgga ataaatgggt
     1381 attcactctg gggggacgct acgattggtc taagcaagcg accactgttc gtgaaaactc
     1441 ttatacgccg actgaaggtt atattgagcg taatgatcat cagttcacct ggcgcggtgg
     1501 tgtaaactac ttattcgata atggtatttc accttacttt agctatagcc agtcctttga
     1561 accgagtgct ttcgatctgt ggagcaaccc gcgcgtttcc tataagccat cgaaaggtga
     1621 acagtatgaa gctggcgtaa aatatgttcc gaatgatatg ccggtcgttg ttacgggcgc
     1681 agtctatcaa ttgacgaaaa caaataacct gacagcagac ccaacaaacc cgttagcgca
     1741 agtcccagca ggtgagattc gcgctcgtgg tgtggaactt gaagcaaaag cggcgttaaa
     1801 tgccaatatt aacttgacgg cttcttatac ctacacggat gcggaataca ccaaagacac
     1861 caatctcaaa ggtaaaactc cagaacaagt accggagcat atggcatctc tctgggggga
     1921 ttataccttc aatgaagggc cgctttctgg tttaacattg ggaacaggtg gtcgttttat
     1981 tggttccagc tatggtgatc cggcaaacac ttttaaagtg ggtagcgcag ctgtaatgga
     2041 tgctgttgta aaatatgatc tggcacgctt tggtatggcg ggatccagcc ttgctgtgaa
     2101 cgtcaacaat ttgctcgatc gtgagtatgt tgccagttgc ttccagacct atggctgctt
     2161 ctggggcgca gaacgtcagg tcgttgcaac cgcaaccttc cgtttctaat ttctcttttg
     2221 gggcacggat atccgtgccc gtttcacaag ttggctgtta tgcaggaata cacgaatcat
     2281 tccgatacca cttttgcact gcgtaatatc tcctttcgtg tgcccgggcg cacgcttttg
     2341 catccgctgt cgttaacctt tccagccggg aaagtgaccg gtctgattgg tcacaacggt
     2401 tctggtaaat ccactctgct caaaatgctt ggccgtcatc agccgccgtc ggaaggggag
     2461 attcttcttg atgcccaacc gctggaaagc tggagcagca aagcgtttgc ccgcaaagtg
     2521 gcttatttgc cgcagcagct tcctccggca gaagggatga ctgtgcgtga actggtggcg
     2581 attggtcgtt acccatggca tggcgcgctg gggcgctttg gtgcggcaga tcgcgaaaag
     2641 gtcgaggaag ctatctcgct ggttggctta aaaccgctgg cgcatcggct ggtcgatagt
     2701 ctctctggcg gtgagcgtca gcgggcgtgg atcgccatgc tggtggcgca ggatagccgt
     2761 tgtctgttgc tcgacgaacc gacctcggcg ttggatatcg cccaccaggt tgatgtcctg
     2821 gcgctggtgc accgtttaag tcaggagcgt ggcctgacgg tcattgccgt gctgcacgat
     2881 atcaatatgg cggcacgcta ctgtgattat ctggtcgccc tgcgcggcgg tgaaatgatt
     2941 gctcagggaa cgcctgcgga aattatgcgc ggcgaaaccc tcgaaatgat ttatggcatc
     3001 ccgatgggta ttttgccgca tccggcgggt gctgcacctg tgagttttgt ttattgatga
     3061 gcggcttacc tcttatttcg cgtcgtcgac tgttaacggc gatggcgctt tctccgttgt
     3121 tatggcagat gaataccgcc cacgcggcgg ctattgatcc caatcgtata gtggcgctgg
     3181 agtggttgcc ggtggaatta ctgctggcgc tcggcatcgt gccttacggc gtggcggata
     3241 ccatcaacta tcgcctgtgg gtcagcgaac caccgttacc tgaatcggtg attgacgtcg
     3301 gtttgcgcac agaacctaac cttgaactgc tgaccgaaat gaaaccgtcg tttatggtct
     3361 ggtcggcagg atatggccct tcaccagaaa tgctggcacg cattgcgccg gggcgcggat
     3421 ttaacttcag cgatggcaaa cagccgctgg cgatggcgcg taaatcgctg acggaaatgg
     3481 cggatttact taacctgcaa agcgcagcgg aaacgcattt agcgcaatat gaagacttta
     3541 tccgcagcat gaaaccccgt tttgtgaagc gtggtgcgcg cccgttactg ctgacgacgc
     3601 ttatcgatcc gcgccatatg ctggtcttcg gtccaaacag cttgttccag gaaattctcg
     3661 atgagtacgg catcccaaat gcctggcaag gggaaaccaa cttctggggc agcaccgccg
     3721 tcagtatcga tcgtctggcg gcgtataaag atgttgatgt gctctgtttt gatcacgaca
     3781 acagcaaaga catggacgcg ctaatggcaa ccccgctgtg gcaggccatg ccgtttgtcc
     3841 gtacagggcg ctttcagcgc gtacccgcag tctggtttta tggtgcgacg ctctcggcaa
     3901 tgcactttgt gcgtattctg gataacgcca tcggaggtaa agcgtgagta aacgaattgc
     3961 gcttcttccg gcgttattgc tggcgctgtt agtgattgtc gctacggcgc tcacctggat
     4021 gaacttctcg caggcgctgc cgcgtagcca gtgggcgcag gctgcctggt cgccggatat
     4081 tgacgtcatc gagcagatga tttttcacta cagcttgttg ccgcgtctgg cgatttcgct
     4141 gctggtgggc gcaggcctgg ggctggtggg cgtgctgttt cagcaagtgc tgcgtaaccc
     4201 gctggcggaa ccgacgacgt tgggcgttgc tacaggcgcg caactgggga ttaccgtcac
     4261 gacgctctgg gcgatccctg gtgcgatggc gagccagttt gctgcgctgg caggggcttg
     4321 tgttgttggc ttaattgtct ttggcgtcgc gtgggggaaa cggctgtcgc cggtaacgct
     4381 gattctcgcg gggctggtag tgagccttta ttgtggggca atcaatcaat tactggttat
     4441 cttccaccat gaccaactgc aaagcatgtt cctgtggagc accggaacgc tgacgcaaac
     4501 cgactggggc ggtgttgagc gtttatggcc gcagttgctg ggcggtgtga tgctgacgtt
     4561 gctgctactt cgtccgttaa ccctgatggg gcttgatgat ggcgtggcgc gcaatctcgg
     4621 gctggccttg tcgctcgcgc gtctggcggc gctgtcgctg gcgattgtca tcagtgcgct
     4681 gctggtgaac gctgtgggga ttatcggctt tatcgggttg ttcgcgccgc tgctggcaaa
     4741 aatgctgggg gcgcggcgtc tgctgccacg attgatgctg gcgtcgctga ttggtgcgtt
     4801 aattctgtgg ctttctgatc aaatcatcct ctggctgact cgcgtgtgga tggaagtttc
     4861 caccggttcg gtcactgcgt tgatcggtgc gccgctgcta ctgtggctgc tgccgcgttt
     4921 acgcagcatt agcgcgccgg atatgaaggt caacgatcgt gtcgcggctg aacgccaaca
     4981 tgtgctggcg tttgccctcg cgggcggcgt gctgctgttg atggctgtgg tggtggcgct
     5041 gtcgtttggt cgtgatgcgc acggctggac gtgggcgagc ggggcgttgc tcgaggattt
     5101 gatgccctgg cgctggccgc gaattatggc ggcgctgttt gcgggcgtca tgctggcggt
     5161 ggcgggctgt attattcagc ggctgaccgg aaacccgatg gcaagcccgg aagtgctggg
     5221 gattagctct ggcgcggcgt ttggcgtggt gttaatgctg tttctggtgc cgggtaatgc
     5281 ctttggctgg ctgttacccg cgggcagtat cggggcggcg gtgacgctgt tgatcattat
     5341 gatcgccgcc ggccgcggtg gattttcccc acaccgtatg ttactggcgg ggatggcgtt
     5401 aagtaccgca ttcaccatgc ttttgatgat gttgcaggcg agcggtgacc cgcgaatggc
     5461 gcaagtgctg acctggattt ccggttcgac ctacaacgcg accgatgcgc aggtctggcg
     5521 taccggaatt gtgatggtga ttttgctggc gattaccccg ctgtgccgcc gctggctgac
     5581 cattttaccg ctgggtggtg ataccgcccg agccgtgggg atggcgctga cgccgacgcg
     5641 aattgcgctg ttgatgttag cggcctgtct gacagcgacc gcgacaatga ccattggacc
     5701 gttgagtttt gttggtttaa tggcaccgca tattgcgcgg atgatgggct ttcgacggac
     5761 gatgccacac atcgtaattt cggcgctggt gggtggttta ttgctggtgt tcgctgactg
     5821 gtgtgggcgg atggtgctgt ttccattcca gatcccggcg gggctgctgt cgacctttat
     5881 cggcgcgcca tattttatct atttgctgag aaagcagagc cgttaatttt tccatataac
     5941 ggccctgtac gcctggacgg ataaagcgtt tacgcgacaa cggatgcccg atgcgacgct
     6001 ggcgcgtctt atcgggccta caaacggccc cgaatcgtag gtcggataag gcgttcacgc
     6061 cgcatccgac aaaccatgct ggatcacaac ttcgcaaaca cccgacgtgc agcatcgatg
     6121 gtgttattga tatcttccat gctgtgcgcc acagacataa agcccgcttc aaacgctgac
     6181 ggtgccaggt aaacaccttc atccagcatc atatggaaga aacgcttaaa acgctcgacg
     6241 tcacaggcca tcacatcctg atagcacgtc acggactcgg cgtcggtaaa gaaaataccg
     6301 aacatgccgc caacgtggtt taccaccagc ggaattccgg cttcttctgc cgcttccagc
     6361 agaccttctg ccagacgtgt tgtcagctca tccagcgttt cgtgaacgcc cggctgcgcg
     6421 acttcattca gacaggcgaa accggctgcc atcgcaatcg ggttaccgga aagcgtaccc
     6481 gcctgataga ccggacccgt cggggccagc gcatccatta catcacgacg accaccgaat
     6541 gcgcctaccg gcattccacc gccgatgatt ttgccgaggc aggttaaatc tggcactacg
     6601 ccgtaataat cctgtgcgcc agctagcgct acgcggaaac cggtcatcac ttcatcgatg
     6661 atcagcaacg cgccaaattc gtcgcacagc gcgcgcagac ctggcaggaa ctctggcagc
     6721 ggtggaacac agttcatatt gcctgccacc ggctcgacga taatacaggc aatctcttgc
     6781 gggtattgct caaacgcggc gcgtacagaa gccagatcgt tataagtaca ggttaaggta
     6841 tgcttggcga aatctgccgg aacgcccggc gagtttggct gacctaacgt gagtgcgcca
     6901 gaaccggctt tcaccagcag gcagtcagcg tgaccgtggt aacaaccttc aaatttaata
     6961 attttgtcgc gaccggtaaa accacgggcc aggcggatgg cgctcatggt cgcctcggtg
     7021 ccggagttca ccatgcgcac catatccatg gtcgggacca gttcggtcac cagttgcgcc
     7081 attttcactt ccatttcggt tggtgcacca aagcttaaac cacgctcggc ggcttcaatc
     7141 acggcattgc ggatcgccgg atggttatgg cccagcacca tcggccccca ggaaccgaca
     7201 taatcgatat aggctttgcc atcaacatcg tacagataag cgccgtccgc tttttcgata
     7261 aacagtggag tgccgcccac gccagtaaag gcgcgaacag gggagttcac accgccaggg
     7321 atcagctcgc gcgctgcgct gtaaagattt tcagacttac tcatggaggg ttcctgattc
     7381 gtagaaaaag tgaatggctg ctattctatg ttattcataa caagttaaat actcgtcaaa
     7441 catcaggctg cttgtactgg tgcaatcctg atttcttaga gtataaaagt tttgtgcatt
     7501 tgaaacatta cgctttgcaa aggattttca tggaacgtgc gagtaaaatg ccgtcatctt
     7561 atttgtatga ccaataagtg atcattggat gaaaactgat actccctctt tagaaacacc
     7621 gcaggccgcg cgcctgcgac gcagacaact gattcgccaa cttcttgagc gcgataaaac
     7681 cccgttagcc attttgttta tggcggcagt cgtcggcacg cttgtcgggc tggctgcggt
     7741 tgcttttgac aaaggtgtcg cctggttgca gaaccaacgt atgggggcgc tggtacatac
     7801 tgctgataat tatccgctac tgttaaccgt tgcttttctc tgttcggcgg tgctggcgat
     7861 gttcggctac tttttggtgc gcaaatacgc gcccgaagca gggggatcgg ggatcccgga
     7921 aattgaaggg gcgctggaag atcaacgtcc tgttcgctgg tggcgtgtat tgccggtgaa
     7981 attctttggc gggctgggga cactcggcgg aggcatggtg ttggggcgcg aagggccaac
     8041 cgtgcagatc ggcggtaata tcgggcggat ggtactcgat gttttccgcc tgaaaggtga
     8101 tgaagctcgc catacgctgt tggcaaccgg tgcggcggcg ggtctggcgg cggcctttaa
     8161 cgcgccgctg gcgggtattt tgtttattat cgaagagatg cgtccgcagt ttcgctatac
     8221 gttaatttcg attaaagcgg tatttattgg cgtcattatg tcgaccatta tgtaccggat
     8281 ttttaatcat gaagttgcgt tgattgacgt cggtaaactt tctgatgcgc cgcttaatac
     8341 gctgtggctt tatctgatcc tcggtattat ttttggcatt ttcggcccta tttttaataa
     8401 atgggtgctg gggatgcagg atttgctgca ccgtgtgcac ggcggcaata ttaccaaatg
     8461 ggtgctaatg ggcggtgcga ttggcggtct gtgtggattg ctggggtttg tggtaccagc
     8521 aacgtcgggc ggcggtttta acctgattcc tatcgctacc gcggggaatt tcagcatggg
     8581 aatgctggtg tttatcttcg tcgcgcgggt cattaccacc ttactctgct tctcttccgg
     8641 cgcgccgggc ggtatttttg ccccgatgct ggcgctgggt actgtgctgg gaaccgcttt
     8701 cggaatggtt gccgttgagc tgtttccgca atatcacctt gaggcgggga catttgctat
     8761 tgccggaatg ggggcgttac tggcggcatc tattcgcgcg ccgttaacgg ggatcattct
     8821 ggttctggag atgaccgata actaccagct cattttgcca atgattatta ccggtcttgg
     8881 cgcaacacta ttagcgcaat ttaccggtgg gaaaccgcta tactcggcga ttcttgcgcg
     8941 aacgctggca aaacaggaag ctgagcaact ggcgcgaagc aaggccgcat cagccagcga
     9001 gaatacttga acgaaatacc agggtattag ataatggcga ttattattgg gttagaattt
     9061 gcccaattgc cgatgtcgtt tggagcaaaa tatgagtgat gacgtagcac tgccgctgga
     9121 gtttaccgac gcagcagcca acaaagttaa aagcctgatc gctgacgaag ataacccgaa
     9181 tctgaaacta cgcgtgtata tcaccggtgg cggttgcagc ggcttccagt atggtttcac
     9241 ctttgatgat caggtgaacg aaggcgatat gaccatcgaa aaacagggcg ttggcctggt
     9301 ggttgacccg atgagcctgc aatatctggt cggcggttct gttgactata ccgaaggtct
     9361 ggaaggttct cgtttcatcg tgaccaaccc gaacgcgaaa agcacctgcg gttgcggttc
     9421 ttcctttagt atctaatctg ttgttcatgt gccggataaa ctatccggca catcttctct
     9481 cttcaacgcc cattctcatc cagcgcaaac gtcggtagct taagatgcca acgaatagcc
     9541 gccagccgaa tcaatagcgt cacgaccatg cccatcatac tggctgtttc cagtggtacg
     9601 gaaaatgtgt aataagccgt agcgtggaca ataccgccga taatacaggc agttgcgtag
     9661 atttctgtac gtaaaatcat ggggatttcg cgggccagaa catcacgaat gatcccgccg
     9721 ccaacgccag taatgacgcc catacaaacc gcgattaacg gaccggcttc cgcattaaag
     9781 gctttattca cgccaatgcc gacaaacacc gccagaccaa cggcgtccag caccggcaac
     9841 atccattttg gtaagcgtct tggctggcgc accagcacga tggtcagcat gctggttacc
     9901 attgcaacga ccagatcggt gggatctttc acccaaaata ccgggccgtg atccagcgcc
     9961 atgtcgcgaa ttgttccgcc gcctactgcg gtaaccacgc ccaataccag aacaccaaaa
    10021 gggtccatac gcaattttcc ggctaacaaa acgccggaga tggcaaatac cgctgtgccg
    10081 actatatcca gccaatagac gagcattgtt caatccccac tgagcaccta aaaaggtcag
    10141 gcgctaatct acctgtgaaa gcgcattaca gagctgttgt gcagcgagga taatacgtgg
    10201 gcttgcacgt tcaaaccagt cactcgtgag aggaataacg ggaattttga gctgttcacc
    10261 ccagtattgt ttgattttag gaatttggtc cggtccgcct gtaatgacaa tcgcctgtgg
    10321 cgagcgtgct aacacctgtt cgcggctaac ttgcggccag ggaacccggc tgtctttaaa
    10381 gatgttttct ccgccacaaa cttcgagtac ctggttttga atcgactctt ttccactggt
    10441 aaatggcgga ttaatgccga attgcagaaa aacacgtttt ttaggtttat cagcatattg
    10501 cgctttcaat tgcgcgtact gatccagcag ggattgcgcg gcttgttcag ccttatccgg
    10561 ttgcggactc cagggggcca gttgacgtaa cgcattggca atttgttcaa tgcttgtcgt
    10621 atcgacccac atcactttta ttcccagcga agccagctgg tcaacctgcc gctcggcatt
    10681 acctccacgc caggcaatca ccagatcggg tttcagcgcg acaatgcgtt ccagattcat
    10741 cccctgccag gtggaaacct gctcaatctt ttgcgcttgt ggaggatagt cggaatagct
    10801 gctgacccca accggcgtga tcccggcggc aaaggcaagt tcagtgttgg cgggagaaag
    10861 cgtgatgacg cgcggtgccg cgttgagcca cagtggcgcg agaaaagaca gggcgaccag
    10921 cgccctgaac agtgacttag ccatgtgcca gtttctgcac cagtgactca accatcaggc
    10981 tggactgttt agcggcaaca gccaggaact catcgaagct aagatgagac tgttgatcgg
    11041 ccacgtcgga gatggcgcgt acgacaacaa acgggacgtt gaaattgtgg cagacatggg
    11101 cgattgccgt cgcttccatc tctacagcaa tggcctgtgg gaagttgtgg cggattttcg
    11161 ccagaccaac agaaccgttg atgaaagcgt cgccgctaac aatcaggcca cgtacagcgt
    11221 taagattcag ttcggcaatg caggcctcag cggcagcgat cagtttatcg tcagctttaa
    11281 agcctgccgg acagcctggt aactgaccgt attcataacc aaatgccgtg acatccgcgt
    11341 cgtgataacg tgcttcgtcc gagacaacga tatcgcccac tttcaacgtt ggtgccaggc
    11401 caccggcaga accggtgtta ataatcacat ctggcttgca gtgttccaac agcaaagtgg
    11461 cacccagcgc cgcagcgact ttaccgatgc ccgatttcag aagcgcaacc tcggttccat
    11521 tcagttggcc ggtatagatt tcgcaaccgc cgagactgat agtttgacgg ttttcgattt
    11581 tgtcacgcag cagcgtaact tcttcttcca ttgcaccaat gatgccgatt ttcatagatt
    11641 tactcgcgat aagcccgatt tgaaggcata gtttaccatg cgcttacggg gaagcgtatt
    11701 tctcacgcgg gagaggacat ggcacagatt gatttccgaa aaaaaataaa ctggcatcgt
    11761 cgttaccgtt caccgcaggg cgttaaaacc gaacatgaga tcctgcggat cttcgagagc
    11821 gatcgcgggc gtatcatcaa ctctccggca attcgtcgtc tgcaacaaaa gacgcaggtt
    11881 tttccactgg agcgcaatgc cgccgtgcgc acgcgtctta cccactcgat ggaagtccag
    11941 caggtggggc gctacatcgc caaagaaatt ttaagccgtc tgaaagagct taaattgctg
    12001 gaagcatacg gcctggatga actgaccgga ccttttgaaa gcattgttga gatgtcatgc
    12061 ctgatgcacg atatcggcaa tccgccgttt ggtcattttg gcgaagcagc gataaatgac
    12121 tggtttcgcc aacgtttgca cccggaagat gccgaaagcc agcctctgac tgacgatcgc
    12181 tgcagcgtgg cggcactacg tttacggaac ggggaagaac cgcttaacga gctgcggcgc
    12241 aagattcgtc aggacttatg tcattttgag gggaatgcac aaggcattcg tctggtgcat
    12301 acattgatgc ggatgaatct cacctgggca caggttggcg gtattttaaa atatacccgt
    12361 ccggcgtggt ggcgtggcga aacgcctgag acacatcact atttaatgaa aaagccgggt
    12421 tattatcttt ctgaagaagc ctatattgcc cggttgcgta aagaacttaa tttggcgctt
    12481 tacagtcgtt ttccattaac ttggattatg gaagctgccg acgacatctc ctattgtgtg
    12541 gcagaccttg aagatgcggt agagaaaaga atatttaccg ttgagcagct ttatcatcat
    12601 ttgcacgaag cgtggggcca gcatgagaaa ggttcgctct tttcgctggt ggttgaaaat
    12661 gcctgggaaa aatcacgctc aaatagttta agccgcagta cggaagatca gttttttatg
    12721 tatttacggg taaacaccct aaataaactg gtaccctatg cggcacaacg atttattgat
    12781 aatctgcctg cgattttcgc cggaacgttt aatcatgcat tattggaaga tgccagcgaa
    12841 tgcagcgatc ttcttaagct atataaaaat gtcgcggtaa aacatgtgtt tagccatcca
    12901 gatgtcgagc agcttgaatt gcagggctat cgggtcatta gcggattatt agagatttat
    12961 cgtcctttat taagcctgtc gttatccgac tttactgaac tggtagaaaa agaacgggtg
    13021 aaacgtttcc ctattgaaac gcgcttattc cacaaactct cgacgcgcca tcggctggcc
    13081 tatgtcgagg ctgtcagtaa attaccgtca gattctcctg agtttccgct atgggaatat
    13141 tattaccgtt gccgcctgct gcaggattat atcagcggta tgaccgacct ctatgcgtgg
    13201 gatgaatacc gacgtctgat ggccgtagaa caataaccag gcttttgtaa agacgaacaa
    13261 taaattttta ccttttgcag aaactttagt tcggaacttc gcgctataaa acgaatctga
    13321 agaacacagc aattttgcgt tatctgttaa tcgagactga aatacatgaa aaaaaccaca
    13381 ttagcactga gtgcactggc tctgagttta ggtttggcgt tatctccgct ctctgcaacg
    13441 gcggctgaga cttcttcagc aacgacagcc cagcagatgc caagccttgc accgatgctc
    13501 gaaaaggtga tgccttcagt ggtcagcatt aacgtagaag gtagcacaac cgttaatacg
    13561 ccgcgtatgc cgcgtaattt ccagcagttc ttcggtgatg attctccgtt ctgccaggaa
    13621 ggttctccgt tccagagctc tccgttctgc cagggtggcc agggcggtaa tggcggcggc
    13681 cagcaacaaa aattcatggc gctgggttcc ggcgtcatca ttgatgccga taaaggctat
    13741 gtcgtcacca acaaccatgt tgttgataac gcgacggtga ttaaagtcca gctgagcgat
    13801 ggtcgtaagt tcgacgcgaa gatggttggc aaagatccgc gctctgatat cgcgctgatc
    13861 caaatccaga acccgaaaaa cctgaccgca attaagatgg cggattctga tgcactgcgc
    13921 gtgggtgatt acaccgtagc gattggtaac ccgtttggtc tgggagagac ggtaacttcc
    13981 gggattgtct ctgcgctggg gcgtagcggc ctgaatgccg aaaactacga aaacttcatc
    14041 cagaccgatg cagcgatcaa ccgtggtaac tccggtggtg cgctggttaa cctgaacggc
    14101 gaactgatcg gtatcaacac cgcgatcctc gcaccggacg gcggcaacat cggtatcggt
    14161 tttgctatcc cgagcaacat ggtgaaaaac ctgacctcgc agatggtgga atacggccag
    14221 gtgaaacgcg gtgagctggg tattatgggg actgagctga attccgaact ggcgaaagcg
    14281 atgaaagttg acgcccagcg cggtgctttc gtaagccagg ttctgcctaa ttcctccgct
    14341 gcaaaagcgg gcattaaagc gggtgatgtg atcacctcac tgaacggtaa gccaatcagc
    14401 agctttgccg cactgcgtgc tcaggtgggt actatgccgg tgggcagcaa actgaccctg
    14461 ggcttgctgc gcgacggtaa gcaggtcaac gtgaatctgg aactgcagca gagcagccag
    14521 aatcaggttg attccagctc catcttcaac ggcattgaag gcgctgagat gagcaacaaa
    14581 ggcaaagatc agggcgtggt agtgaacaac gtgaaaacgg gcactccggc tgcgcagatc
    14641 ggcctgaaga aaggtgatgt gattattggc gcgaaccagc aggcagtgaa aaacatcgct
    14701 gaactgcgta aagttctcga cagcaaaccg tctgtgctgg cactgaacat tcagcgcggc
    14761 gacagcacca tctacctgtt aatgcagtaa tctccctcaa ccccttcctg aatacgggaa
    14821 ggggttctcc ttacaatctg tgaacttcgc cacaactcca tacatcttca tcatcctttg
    14881 ggcatttgca caatgccgta cgttacgtac ttccttatgc taagccgtgc ataacggagg
    14941 acttatggct ggctggcatc ttgataccaa aatggcgcag gatatcgtgg cacgtaccat
    15001 gcgcatcatc gataccaata tcaacgtaat ggatgcccgc gggcgaatta tcggcagcgg
    15061 cgatcgtgag cgtattggtg aattgcacga aggtgcattg ctggtacttt cacagggacg
    15121 agtcgtcgat atcgatgacg cggtggcacg tcatctgcac ggtgtgcggc aagggattaa
    15181 tctaccgtta cggctggaag gtgaaattgt cggcgtaatt ggcctgacag gtgaaccaga
    15241 gaatctgcgt aaatatggcg aactggtctg catgacggct gaaatgatgc tggaacagtc
    15301 gcggttgatg cacttgttgg cgcaggatag ccgtttgcgg gaggaactgg tgatgaacct
    15361 gattcaggca gaggagaata ctcccgcact tactgaatgg gcgcaacggc tggggatcga
    15421 tctcaatcaa ccgcgagtgg tggctattgt tgaggtcgac agcggtcagc ttggcgtgga
    15481 cagcgcaatg gcggagttac aacaactgca aaacgcgctg actacgcccg agcgtaataa
    15541 tctggtggca attgtctcgc tgaccgaaat ggtggtgttg aaaccggcgt tgaactcttt
    15601 tgggcgctgg gatgcagaag atcatcgtaa gcgagttgaa caactgatta cccgcatgaa
    15661 agagtacggc cagctgcgtt ttcgcgtttc actgggcaac tattttaccg gtcctggcag
    15721 tattgcccga tcctatcgta cggcgaaaac gacgatggtg gtgggtaaac agcggatgcc
    15781 agaaagtcgc tgctattttt atcaggatct gatgttacct gtgttactcg acagtttgcg
    15841 tggcgactgg caggccaacg aactggcgcg accgctggcg cggctgaaag cgatggacaa
    15901 taacggcttg ctgcgacgaa cgctggcggc gtggtttcgt cacaatgtgc aaccgctggc
    15961 aacgtcaaag gcgttgttta ttcatcgtaa taccctggag tatcggctta atcgtatatc
    16021 ggaactgacc gggcttgatt tgggcaattt tgatgacagg ttgctgctgt atgtggcgtt
    16081 acaactggat gaagagcggt aggttatgcg ttaaggttga ggcggcgctg gctcattccc
    16141 ctcagcccga aggggagagg ggaatgcccg aattaaccgt caattcggga ttatttacgc
    16201 gttagttttt ccagatcggc ttcaatctcg ctgatcttat tggttacgac tgactccagg
    16261 tgacgtaagt cgtcgaggat cttacgctta agatcaactt cgctgcggtc acgctggcag
    16321 atttgatcaa gctcatcaat gatataccgt agattcgggc tgatttcctg gacttcttta
    16381 taaccctgac ccacaccatc agcgacgacc gttttacgct gacgcggata tttaaactta
    16441 acgctcttgg cgaaaaactc gcctttgtct ttctggaaat agattttcag aatatcgttg
    16501 ttggcttcct gccggaggct gtaacgatca atttcttcag gattggtaat acccagactt
    16561 ttcagattgt cgtacatagc gttacctcaa aatgagtcag taaattgtgc ttattttagc
    16621 atttggcctg gcccgcccgg ctcgactgtg atcgcagaaa gcctttttct tccagacttc
    16681 tatcatggcg taaaaatcaa aaaattacct gctttattgt ggcgatattc catgggatgg
    16741 attagcacat ttatcttcgt tttgcgaatt acatgacagt ccgaaacaag atgaaaaagt
    16801 atttaaaaaa gggggaggaa gaatgagtag gacgaattat tctgaaccag aaagcaatcg
    16861 ggcagcgtcg tgctgcccgt gtgcatactt ttagtcgatg gtacgcagca gttcgttaat
    16921 gccgactttg ccgcgagttt tcgcgtcaac tttcttaacg ataaccgcac agtagaggct
    16981 gtatttgcca tctttcgacg gcagattacc tgaaacaacc acagaccccg ccggaacgcg
    17041 accgtagtgg atttcgccgg tttcacggtc gtaaatacgg gtgctctgac caatgtatac
    17101 gcccatggaa atgacggaac cttcttcgac aatcacccct tcaaccactt cagagcgcgc
    17161 gccgatgaag caattatctt caatgatggt tgggttagcc tgcagcggtt ccagcacacc
    17221 gccgatgccc acgccaccgg aaaggtggac gtttttacca atctgcgcac aagaaccgac
    17281 ggtcgcccag gtatcaacca tggtgccttc atcaacatat gcaccgatgt tgacgtaaga
    17341 cggcatcagc acggtgttac gggcaataaa tgcaccctga cgtaccgccg ctggtggcac
    17401 aacgcggaag ccttctttct gaaaacgcgc ttcatcgtag ttggcgaatt tcatcggcac
    17461 tttatcgaag tagcggcttt ctgccccttc gatcacctga ttatcattaa tacggaaaga
    17521 gagcagcacc gcttttttca accactgatg cgtcacccac tgaccgtcaa ttttttccgc
    17581 tacacgcagt gcgccggaat ccagcagggc gatcacctga tttaccgctt cgcgggtaac
    17641 ggtgtctgcg ttggctggcg tgatctcggc acggcgttca aaagcggttt caataatgtt
    17701 ctgtaactgc tgcattgtta aactcttttc atatcagtaa acacatcacc ctttatcgtt
    17761 tggattgagg gcctctgtca accgctgatg cacttcctgc tgcaactcgt tattaagtgc
    17821 acgccggtca gcggtggcaa ttatgaataa atcttctact cgctcgccaa tggttgtaat
    17881 tcgggcacca tgaagcgaaa ttcccagatc ggcaaaaatt ttcccgactc tcgccagcag
    17941 tcctggctgg tcgagcgcga taagctcaag gaacgatttg cggtcggtat gggttggcaa
    18001 aaacgtcact tcggtttcaa cagtaaagtg acgtagttta gccggttgac gacgcggctg
    18061 tggtggctgc cagctacttt gcgtcagcac ctgctccaga ccaaaccgaa taacctcatg
    18121 gcgatctgcg gacagcgggc tgccatcggg ttccagcacg ataaaggtat ccatcgccat
    18181 accgtcgcga gtggtgaaaa tttgtgcgtc gtgaacgctt aagttgcgtc tatctaactc
    18241 ggcacaaaca gcagcaaaca agtaagggcg gtccgggctc cagataaaaa tctcggtgcc
    18301 tccacgcgta gcctgcgggc taagcaatac cagcggtttg cttaaatcat gctgtaataa
    18361 atggcgcgca tgccaggcca gttgatttgg gctatggcgg acaaaatagt tagcacgaca
    18421 gcgtgaccaa atttggtgca gcgcctcttc gtcgatgtta tccatgcgca gtagtgccag
    18481 cgcctggagt tggtgatggc gaacccgttc gcgcatatcc ggcgtgtttt gcattccgcg
    18541 tcgtagctgt ttttcggtgg caaaatagag ttcgcgcaac agactttgct tccagctatt
    18601 ccacagcgtt tcgttggtgg cgcatatatc ggccactgtc aggcatacca gataacgaag
    18661 tcggttttct gtttgcactt cttcggcaaa ctgcttgatg acttcagggt cctgaatatc
    18721 gcggcgttgt gctgtcaccg acatcagcaa gtgctggcga accagccagg caaccagctg
    18781 tgtttcgcgt gagttcagcc cgtggagctc tgcaaaatgc actacatcct gagcaccgag
    18841 aatggagtgg tcgccgccgc gtcctttggc gatatcgtga aacagcgcgg cgatgaaaat
    18901 cagctcaggt gacggcaggc gcggccagac gtccacacac aacggatggc gctggcgcgt
    18961 ttcttcactg gcaaaactct ccagtttcag catcacgcgg atagtatgtt catccaccgt
    19021 gtaggcgtgg aacagatcaa actgcatctg cccgacgata tgcgaccatt gcggcatata
    19081 cgcgccgagc acgctatggc gatgcattgg caatagcccg cgccgcaccg ctccggggtg
    19141 acgcagaatg ctcaaaaaca gttttcgtgc ttccggaata ttacacagcg gttgttgcag
    19201 atggcgacgg gcatggcgta actggcgcag tgtggtggag taaatgccgg tgatcgcact
    19261 gttgcgcacc atggtgtaga acatacgcaa gatggcttcc ggctggcgca taaatagtgt
    19321 ttcatcacgc aggtcgatta gcgtaccgcg tagctgaaac tcatcgtcga ttggacgtgg
    19381 tttttcgtcg gcgggaaggg cgaggatggc ttcatcgaac agttgcagca gcatctggtt
    19441 gagttcactg acgcggcgtg tcacgcggaa gtaatccttc atcatccgct cgaccggttc
    19501 gttaccttcg ccactgtaat tcagacgctg ggcgacgcta agctggcgat cgaataacag
    19561 gcgattatcg taacggctga cgaccagatg cagggcaaag cgaatacgcc acaatatatg
    19621 cagacattcg tttaattccg cccgctccgc tgaggttaag aagccaaacc caaccatttc
    19681 atccagcgat gttgcgccaa aatgacggcg ggccacccat tgcagagtgt ggatatcgcg
    19741 caagccgcca gggctacttt tgatgtctgg ttcaagattg tagctggtgc cgtggtaacg
    19801 ctgatggcgc tggttctgtt cttcaacttt cgccgcgtag aacttgtcgg aaggccagaa
    19861 tccttcgcta aaaatatgtt tttgcagttc aagaaatagc gcaacatcgc caattaataa
    19921 gcgggattcg atcaaattgg tggcgacggt taaatccgat aacccttcca gcatgcactc
    19981 ttcaagcgtg cgcacgctat gacccacttc cagctttaca tcccagagca gcgttaacag
    20041 ctcgcccact ttttgcgcct gatcgtccgg gagcttttta cggcttaaaa tcagtaaatc
    20101 gatgtcagaa agtggatgca gctcgccacg accgtagcca ccgacggcga ccaatgccag
    20161 gtcggcaatc tggctgaatc ccgcttcaat ccataatcgt tgcaggagct ggtcgataaa
    20221 ctcggtgcgc gcctcaatca actgttctgc agagatccca ttgtcaaagg catcacccag
    20281 ccaacgctgg aaagtatcga tatgggcttt tatcccaccg acggttaatt catcacgggg
    20341 ccagacgcat ggattttgcg gttgaccggg cagggtgggg agagcggtgt ttgcgtactg
    20401 ttctggaagg gtattcattg tgcgccaccc ataagattaa attatcgcat taaaaaagcc
    20461 ggcattcgcc ggcttcatct tattcgtcgt gcgagattat cgccgggatg gtgtcatcct
    20521 tgcgtagcgt cagaatttcg cagccgttat cagtcaccac aatagtatgc tcatattgtg
    20581 cagacaagct gcgatctttg gtttttaccg tccagccatc tttcatggtg cggatctctt
    20641 ttttacccgc gttgaccatt ggctcgatgg tgaacgtcat cccaggtttc agtacgacgt
    20701 tggtttcacg ggagtcatag tgcagcacct gcggttcttc atggaagccg cgaccaatac
    20761 cgtgtccgca atattcacga acgacggaga agccttctgc ttcgacaaat ttctgaatcg
    20821 ccgcaccgat ttcgcgcaga ttaatgcctg gttttaccat gcgtagcgcc aggtacaggc
    20881 tttcttgcgt gatgcggcac agacgttcgc ccatgatggt cggcttaccg acgataaaca
    20941 ttttcgaggt atcgccgtgg aaaccatctt tgattacggt gacatcaatg ttaacgatat
    21001 cgccatcttt cagcagctta gcatcgtccg ggataccgtg gcacaccact tcattaatag
    21061 aaatgcaaac ggatttcgga taaccgtgat agccgaggca ggcagaaacc gcgtgttgtt
    21121 cattaacaat gtaatcatta cagatgcgat ccagctcgcc ggtgctgacg cccggtttaa
    21181 catatggttc gatcatctcc agcacttcgg cagccagtcg gccagcgacg cgcatttttt
    21241 cgatatcttc tggggtcttg attgagatag ccattaattc tgtccatcag cgtcggtgat
    21301 accgacaata tatatgtaag tgccgtcaat ggtatcacac ccgggcaaat tgagaatcat
    21361 tctgaatttc gccaaacgtg ccactgaagg ttttctataa tagaaaattc gacgtctgat
    21421 gctgtacaca gcgccaacaa ttattggtgt cccagacgta tttgtggtat aaagcgcgcc
    21481 ggacttccga tccatttcgt atacacagac tggacggaag cgacaatctc actttgtgta
    21541 acaacacaca cgtatcggca catattccgg ggtgcccttt ggggtcggta atatgggata
    21601 cgtggaggca taaccccaac ttttatatag aggttttaat catggcaact gtttccatgc
    21661 gcgacatgct caaggctggt gttcacttcg gtcaccagac ccgttactgg aacccgaaaa
    21721 tgaagccgtt catcttcggt gcgcgtaaca aagttcacat catcaacctt gagaaaactg
    21781 taccgatgtt caacgaagct ctggctgaac tgaacaagat tgcttctcgc aaaggtaaaa
    21841 tccttttcgt tggtactaaa cgcgctgcaa gcgaagcggt gaaagacgct gctctgagct
    21901 gcgaccagtt cttcgtgaac catcgctggc tgggcggtat gctgactaac tggaaaaccg
    21961 ttcgtcagtc catcaaacgt ctgaaagacc tggaaactca gtctcaggac ggtactttcg
    22021 acaagctgac caagaaagaa gcgctgatgc gcactcgtga gctggagaaa ctggaaaaca
    22081 gcctgggcgg tatcaaagac atgggcggtc tgccggacgc tctgtttgta atcgatgctg
    22141 accacgaaca cattgctatc aaagaagcaa acaacctggg tattccggta tttgctatcg
    22201 ttgataccaa ctctgatccg gacggtgttg acttcgttat cccgggtaac gacgacgcaa
    22261 tccgtgctgt gaccctgtac ctgggcgctg ttgctgcaac cgtacgtgaa ggccgttctc
    22321 aggatctggc ttcccaggcg gaagaaagct tcgtagaagc tgagtaataa ggcacgctcg
    22381 tacgagccct tatagaccag gtagtacacg tttggttagg gggcctgcat atggccccct
    22441 ttttcacttt tatatctgtg cggtttaatg ccgggcagat cacatctccg aggattttag
    22501 aatggctgaa attaccgcat ccctggtaaa agagctgcgt gagcgtactg gcgcaggcat
    22561 gatggattgc aaaaaagcac tgactgaagc taacggcgac atcgagctgg caatcgaaaa
    22621 catgcgtaag tccggtgcta ttaaagcagc gaaaaaagca ggcaacgttg ctgctgacgg
    22681 cgtgatcaaa accaaaatcg acggcaacta cggcatcatt ctggaagtta actgccagac
    22741 tgacttcgtt gcaaaagacg ctggtttcca ggcgttcgca gacaaagttc tggacgcagc
    22801 tgttgctggc aaaatcactg acgttgaagt tctgaaagca cagttcgaag aagaacgtgt
    22861 tgcgctggta gcgaaaattg gtgaaaacat caacattcgc cgcgttgctg cgctggaagg
    22921 cgacgttctg ggttcttatc agcacggtgc gcgtatcggc gttctggttg ctgctaaagg
    22981 cgctgacgaa gagctggtta aacacatcgc tatgcacgtt gctgcaagca agccagaatt
    23041 catcaaacca gaagacgtat ccgctgaagt ggtagaaaaa gaataccagg tacagctgga
    23101 tatcgcgatg cagtctggta agccgaaaga aatcgcagag aaaatggttg aaggccgcat
    23161 gaagaaattc accggcgaag tttctctgac cggtcagccg ttcgttatgg aaccaagcaa
    23221 aactgttggt cagctgctga aagagcataa cgctgaagtg actggcttca tccgtttcga
    23281 agtgggtgaa ggcatcgaga aagttgagac tgactttgca gcagaagttg ctgcgatgtc
    23341 caagcagtct taattatcaa aaaggagccg cctgagggcg gcttcttttt gtgcccatct
    23401 tgtaaattca gctaaccctt gtggggctgc gctgaaaagc gacgtacaat gtcgctagta
    23461 ttaattcatt tcaatcgttg acagtctcag gaaagaaaca tggctaccaa tgcaaaaccc
    23521 gtctataaac gcattctgct taagttgagt ggcgaagctc tgcagggcac tgaaggcttc
    23581 ggtattgatg caagcatact ggatcgtatg gctcaggaaa tcaaagaact ggttgaactg
    23641 ggtattcagg ttggtgtggt gattggtggg ggtaacctgt tccgtggcgc tggtctggcg
    23701 aaagcgggta tgaaccgcgt tgtgggcgac cacatgggga tgctggcgac cgtaatgaac
    23761 ggcctggcaa tgcgtgatgc actgcaccgc gcctatgtga acgctcgcct gatgtccgct
    23821 attccattga atggcgtgtg cgacagctac agctgggcag aagctatcag cctgttgcgc
    23881 aacaaccgtg tggtgatcct ctccgccggt acaggtaacc cgttctttac caccgactca
    23941 gcagcttgcc tgcgtggtat cgaaattgaa gccgatgtgg tgctgaaagc aaccaaagtt
    24001 gacggcgtgt ttaccgctga tccggcgaaa gatccaaccg caaccatgta cgagcaactg
    24061 acttacagcg aagtgctgga aaaagagctg aaagtcatgg acctggcggc cttcacgctg
    24121 gctcgtgacc ataaattacc gattcgtgtt ttcaacatga acaaaccggg tgcgctgcgc
    24181 cgtgtggtaa tgggtgaaaa agaagggact ttaatcacgg aataattccc gtgatggata
    24241 aataagggta agattccgcg taagtatcgc gggggcgtaa gtctggttat aaggcgttat
    24301 tgttgcaggc agtttggtca cggccagcgc gcagcaaccg gagcgtacaa aagtacgtga
    24361 ggatggcgag cactgcccgg ggccaaaatg gcaaataaaa tagcctaata atccagacga
    24421 ttacccgtaa tatgtttaat cagggctata cttagcacac ttccactgtg tgtgactgtc
    24481 tggtctgact gagacaagtt ttcaaggatt cgtaacgtga ttagcgatat cagaaaagat
    24541 gctgaagtac gcatggacaa atgcgtagaa gcgttcaaaa cccaaatcag caaaatacgc
    24601 acgggtcgtg cttctcccag cctgctggat ggcattgtcg tggaatatta cggcacgccg
    24661 acgccgctgc gtcagctggc aagcgtaacg gtagaagatt cccgtactct gaaaatcaac
    24721 gtgtttgatc gttcaatgtc tccggccgtt gaaaaagcga ttatggcgtc cgatcttggc
    24781 ctgaacccga actctgcggg tagcgacatc cgtgttccgc tgccgccgct gacggaagaa
    24841 cgtcgtaaag atctgaccaa aatcgttcgt ggtgaagcag aacaagcgcg tgttgcagta
    24901 cgtaacgtgc gtcgtgacgc gaacgacaaa gtgaaagcac tgttgaaaga taaagagatc
    24961 agcgaagacg acgatcgccg ttctcaggac gatgtacaga aactgactga tgctgcaatc
    25021 aagaaaattg aagcggcgct ggcagacaaa gaagcagaac tgatgcagtt ctgatttctt
    25081 gaacgacaaa aacgccgctc agtagatcct tgcggatcgg ctggcggcgt tttgcttttt
    25141 attctgtctc aactctggat gtttcatgaa gcaactcacc attctgggct cgaccggctc
    25201 gattggttgc agcacgctgg acgtggtgcg ccataatccc gaacacttcc gcgtagttgc
    25261 gctggtggca ggcaaaaatg tcactcgcat ggtagaacag tgcctggaat tctctccccg
    25321 ctatgccgta atggacgatg aagcgagtgc gaaacttctt aaaacgatgc tacagcaaca
    25381 gggtagccgc accgaagtct taagtgggca acaagccgct tgcgatatgg cagcgcttga
    25441 ggatgttgat caggtgatgg cagccattgt tggcgctgct gggctgttac ctacgcttgc
    25501 tgcgatccgc gcgggtaaaa ccattttgct ggccaataaa gaatcactgg ttacctgcgg
    25561 acgtctgttt atggacgccg taaagcagag caaagcgcaa ttgttaccgg tcgatagcga
    25621 acataacgcc atttttcaga gtttaccgca acctatccag cataatctgg gatacgctga
    25681 ccttgagcaa aatggcgtgg tgtccatttt acttaccggg tctggtggcc ctttccgtga
    25741 gacgccattg cgcgatttgg caacaatgac gcccgatcaa gcctgccgtc atccgaactg
    25801 gtcgatggga cgtaaaattt ctgtcgattc ggctaccatg atgaataaag gtctggaata
    25861 cattgaagcg cgttggctgt ttaacgccag cgccagccag atggaagtgc tgattcaccc
    25921 gcagtcagtg attcactcaa tggtgcgcta tcaggacggc agtgttctgg cgcagctggg
    25981 ggaaccggat atgcgtacgc caattgccca caccatggca tggccgaatc gcgtgaactc
    26041 tggcgtgaag ccgctcgatt tttgcaaact aagtgcgttg acatttgccg caccggatta
    26101 tgatcgttat ccatgcctga aactggcgat ggaggcgttc gaacaaggcc aggcagcgac
    26161 gacagcattg aatgccgcaa acgaaatcac cgttgctgct tttcttgcgc aacaaatccg
    26221 ctttacggat atcgctgcgt tgaatttatc cgtactggaa aaaatggata tgcgcgaacc
    26281 acaatgtgtg gacgatgtgt tatctgttga tgcgaacgcg cgtgaagtcg ccagaaaaga
    26341 ggtgatgcgt ctcgcaagct gaggataatc cggctacaga gagtcgcgct atttgttagc
    26401 gtagggcttc agtgatatag tctgcgccat ctgatcgtaa gtagttggct ttataaggtc
    26461 aggtatgccg tggttttaca cggctttttt ttgtataggc ttcagtattc ctgagtaccg
    26521 taaaccctgt cagggaataa aaaacgcgtg atgttgtctg ctactcaacc acttagcgaa
    26581 aaattgccag cgcatggctg ccgtcatgtt gcgatcatta tggacggcaa tggtcgctgg
    26641 gcaaaaaagc aagggaagat tcgtgccttt gggcataaag ccggggcaaa atccgtccgc
    26701 cgggctgtct cttttgcggc caacaacggt attgaggcgt taacgctgta tgcctttagt
    26761 agtgaaaact ggaaccgacc agcgcaggaa gtcagtgcgt taatggaact gtttgtgtgg
    26821 gcgctcgata gcgaagtaaa aagtctgcac cgacataacg tgcgtctgcg tattattggc
    26881 gataccagtc gttttaactc gcgtttgcaa gaacgtattc gtaaatctga agcgctaaca
    26941 gccgggaata ccggtctgac gctgaatatt gcggcgaact acggtggacg ttgggatata
    27001 gtccagggag tcaggcaact ggctgaaaag gtgcagcaag gaaacctgca accagatcag
    27061 atagatgaag agatgctaaa ccagcatgtc tgtatgcatg aactggcccc tgtagattta
    27121 gtaattagga ctggggggga gcatcgcatt agtaactttt tgctttggca aattgcctat
    27181 gccgaacttt actttacaga tgttctctgg cccgatttcg atgaacaaga ctttgaaggg
    27241 gcgttaaatg cctttgctaa tcgagagcgt cgtttcggcg gcaccgagcc cggtgatgaa
    27301 acagcctgat gggggtcgct tttgctgaag tatcgcctga tatctgcttt tgtgttaata
    27361 cccgtcgtca tcgcggcgtt gtttctgttg ccgccggtgg ggttcgccat cgtaacgctg
    27421 gtggtctgca tgctggcagc gtgggaatgg ggacagctta gcggttttac cactcgttcg
    27481 cagcgagtat ggttggcggt gttatgcggg ttattgttgg cgcttatgct ttttctgttg
    27541 ccggaatatc accgaaatat tcatcaaccg ctggttgaaa tctcactttg ggcttcgctg
    27601 ggttggtgga ttgtcgcgct attgctggtg ctgttttacc caggttccgc agcaatctgg
    27661 cgtaactcta aaacattgcg ccttattttt ggcgtgctaa ccattgttcc cttcttctgg
    27721 ggcatgctgg cgttacgggc ctggcactac gacgagaatc attacagtgg cgcaatatgg
    27781 ctgctctatg tcatgatcct ggtatggggc gctgactctg gcgcatatat gtttggcaaa
    27841 ttgtttggta aacataagct ggcaccgaag gtttctccgg gtaaaacctg gcaaggcttt
    27901 atcggtggac tcgctactgc agcggtaatc tcatggggtt atggcatgtg ggcgaatctc
    27961 gacgtcgctc ccgtcacctt actcatttgc tctattgtcg cagcgttagc ctcagtgctc
    28021 ggcgatctga ccgagagtat gtttaagcgt gaagcaggaa ttaaggacag cggtcattta
    28081 attccaggac acggtggtat tttagatcgt attgatagcc tgacggctgc ggtaccggtc
    28141 tttgcttgct tgttgttact ggtattcagg acgctttaac ggaaggtaat atgctgagtt
    28201 ttctctggga tttggcttcg ttcatcgttg cactgggtgt acttatcacc gtgcatgaat
    28261 ttggtcattt ctgggttgcc cggcgttgtg gtgttcgcgt tgagcgtttc tcaatagggt
    28321 ttggtaaggc gctctggcgg cgaactgata agctcggcac cgaatatgtt atcgccctga
    28381 tcccgttggg cggttatgtc aaaatgctgg atgagcgcgc agaaccggtc gttccggaac
    28441 tccgccacca tgccttcaat aataaatctg tcggccaacg agcggcgatt attgccgcag
    28501 gtccggttgc aaacttcatt tttgctatct ttgcctactg gctggttttt attattggtg
    28561 tgcctggcgt acgtccggtg gttggcgaaa tagcagccaa ttcgatagct gcggaagcac
    28621 aaattgcacc aggtacggaa ctaaaagccg tagatggtat cgaaacgcct gattgggatg
    28681 ccgtgcgttt gcagttagtc gataaaattg gcgatgaaag caccaccatt acagtagcgc
    28741 catttggcag cgaccaacgg cgggatgtaa agctcgattt acgtcactgg gcgtttgagc
    28801 ctgataaaga agatccggta tcttcgctgg ggattcgtcc tcgtgggccg caaattgaac
    28861 ctgtactgga aaatgtgcag ccaaactcgg cggcaagcaa ggcaggtttg caagcaggcg
    28921 acaggatcgt taaagtcgat ggtcagccct taacgcagtg ggtgaccttt gtgatgcttg
    28981 tccgggataa cccgggtaaa tccttagcgt tagaaatcga aaggcagggg agtcccttgt
    29041 ctttgacatt aatcccggag agtaaaccgg gtaatggtaa agcgattggt tttgtcggta
    29101 ttgagccgaa agtcattcct ttgccagatg agtataaagt tgtacgccag tatgggccgt
    29161 tcaacgccat cgtcgaagcc acggacaaaa cgtggcagct gatgaagctg acggtcagta
    29221 tgctgggaaa attgatcacc ggtgatgtga aactgaacaa cctcagtggg ccgatctcta
    29281 tcgccaaggg ggctgggatg acagcggaac tcggggtagt ttattacctg ccgtttcttg
    29341 cgcttattag cgtgaactta gggataatta acctgtttcc gttgcccgta cttgacgggg
    29401 ggcatctgct gttccttgcg atcgaaaaga tcaagggcgg accggtatcc gagcgggttc
    29461 aagacttttg ttatcgcatt ggctcgattc tgctggtgct gttaatgggg cttgcacttt
    29521 tcaatgattt ctctcggtta tgagagttag ttaggaagaa cgcataataa cgatggcgat
    29581 gaaaaagttg ctcatagcgt cgctgctgtt tagcagcgcc accgtatacg gtgctgaagg
    29641 gttcgtagtg aaagatattc atttcgaagg ccttcagcgt gtcgccgttg gtgcggccct
    29701 cctcagtatg ccggtgcgca caggcgacac ggttaatgat gaagatatca gtaataccat
    29761 tcgcgctctg tttgctaccg gcaactttga ggatgttcgc gtccttcgtg atggtgatac
    29821 ccttctggtt caggtaaaag aacgtccgac cattgccagc attactttct ccggtaacaa
    29881 atcggtgaaa gatgacatgc tgaagcaaaa cctcgaggct tctggtgtgc gtgtgggcga
    29941 atccctcgat cgcaccacca ttgccgatat cgagaaaggt ctggaagact tctactacag
    30001 cgtcggtaaa tatagcgcca gcgtaaaagc tgtcgtgacc ccgctgccgc gcaaccgtgt
    30061 tgacctaaaa ctggtgttcc aggaaggtgt gtcagctgaa atccagcaaa ttaacattgt
    30121 tggtaaccat gctttcacca ccgacgaact gatctctcat ttccaactgc gtgacgaagt
    30181 gccgtggtgg aacgtggtag gcgatcgtaa ataccagaaa cagaaactgg cgggcgacct
    30241 tgaaaccctg cgcagctact atctggatcg cggttatgcc cgtttcaaca tcgactctac
    30301 ccaggtcagt ctgacgccag ataaaaaagg tatttacgtc acggtgaaca tcaccgaagg
    30361 cgatcagtac aagctttctg gcgttgaagt gagcggcaac cttgccgggc actccgctga
    30421 aattgagcag ctgactaaga tcgagccggg tgagctgtat aacggcacca aagtgaccaa
    30481 gatggaagat gacattaaaa agcttctcgg tcgctatggt tatgcctatc cgcgcgtaca
    30541 gtcgatgccc gaaattaacg atgccgacaa aaccgttaaa ttacgcgtga acgttgatgc
    30601 gggtaaccgt ttctacgtgc gtaagatccg ttttgaaggt aacgatacct cgaaagatgc
    30661 cgtcctgcgt cgcgaaatgc gtcagatgga aggtgcatgg ctggggagcg atctggtcga
    30721 tcagggtaag gagcgtctga atcgtctggg cttctttgaa actgtcgata ccgataccca
    30781 acgtgtaccg ggtagcccgg accaggtaga tgtagtctac aaggtgaaag aacgtaacac
    30841 cggtagcttc aacttcggga ttggttacgg tactgaaagt ggcgtgagct tccaggctgg
    30901 cgtgcaacag gataactggt taggtacagg ttatgctgtt ggtatcaacg ggaccaaaaa
    30961 cgattaccag acctatgctg aattgtcggt aaccaacccg tacttcactg tagatggtgt
    31021 aagcctcggt ggtcgtctct tctataatga cttccaggca gatgacgccg acctgtccga
    31081 ctataccaac aagagttatg gtacagacgt gacgttgggc ttcccgatca acgaatataa
    31141 ctcgctgcgt gcaggtctgg gttatgtaca taactccctg tccaacatgc agcctcaggt
    31201 tgcgatgtgg cgttatctgt actctatggg tgaacatccg agcacctctg atcaggataa
    31261 cagcttcaaa acggacgact tcacgttcaa ctatggttgg acctataaca agcttgaccg
    31321 tggttacttc ccgacagatg gttcacgtgt caacctgacc ggtaaagtga ccattccggg
    31381 atcggataac gaatactaca aagtgacgtt agacacggcg acttatgtgc cgatcgatga
    31441 cgatcacaaa tgggttgttc tgggtcgtac ccgctggggt tatggtgatg gtttaggcgg
    31501 caaagagatg ccgttctacg agaacttcta tgccggtggt tccagcaccg tgcgtggctt
    31561 ccagtccaat accattggtc cgaaagcagt ttacttcccg catcaggcca gtaattatga
    31621 tccggactat gattacgaat gtgcgactca ggacggcgcg aaagacctgt gtaaatcgga
    31681 tgatgctgta ggcggtaacg ccatggcggt tgccagcctc gagtttatca ccccgacgcc
    31741 gtttattagc gataagtatg ctaactcggt tcgtacttcc ttcttctggg atatgggtac
    31801 cgtttgggat acaaactggg attccagcca atattctgga tatccggact atagtgatcc
    31861 aagcaatatc cgtatgtctg cgggtatcgc attacaatgg atgtccccat tggggccgtt
    31921 ggtgttctcc tacgcccagc cgttcaaaaa gtacgatgga gacaaggcag aacagttcca
    31981 gtttaacatc ggtaaaacct ggtaagtgtt ctccacaaag gaatgtagtg gtagtgtagc
    32041 gatgacttta ggcgatcaat ataagatcgc cgggccacgc aaagaactgc accctccggt
    32101 gcaaatggga tggtaaggag tttattgtga aaaagtggtt attagctgca ggtctcggtt
    32161 tagcactggc aacttctgct caggcggctg acaaaattgc aatcgtcaat atgggcagcc
    32221 tgttccagca ggtagcgcag aaaaccggtg tttctaacac gctggaaaat gagttcaaag
    32281 gccgtgccag cgaactgcag cgtatggaaa ccgatctgca ggctaaaatg aaaaagctgc
    32341 agtccatgaa agcgggcagc gatcgcacta agctggaaaa agacgtgatg gctcagcgcc
    32401 agacttttgc tcagaaagcg caggcttttg agcaggatcg cgcacgtcgt tccaacgaag
    32461 aacgcggcaa actggttact cgtatccaga ctgctgtgaa atccgttgcc aacagccagg
    32521 atatcgatct ggttgttgat gcaaacgccg ttgcttacaa cagcagcgat gtaaaagaca
    32581 tcactgccga cgtactgaaa caggttaaat aagtaatgcc ttcaattcga ctggctgatt
    32641 tagcgcagca gttggatgca gaactacacg gtgatggcga tatcgtcatc accggcgttg
    32701 cgtccatgca atctgcacaa acaggtcaca ttacgttcat ggttaaccca aaataccgtg
    32761 agcatttagg cttgtgccag gcgtccgcgg ttgtcatgac ccaggacgat cttcctttcg
    32821 cgaaaagtac cgcgctggta gtgaagaatc cctacctgac ttacgcgcgc atggcgcaaa
    32881 ttttagatac cacgccgcag cccgcgcaga acattgcacc cagtgcggtg atcgacgcga
    32941 cggcgaagct gggtaacaac gtatcgattg gcgctaacgc ggtgattgag tccggcgttg
    33001 aactgggcga taacgtgatt atcggtgccg gttgcttcgt aggtaaaaac agcaaaatcg
    33061 gtgcaggttc gcgtctctgg gcgaacgtaa ccatttacca tgagatccag atcggtcaga
    33121 attgcctgat ccagtccgga acagtggtag gcgcagacgg ctttggttat gccaacgatc
    33181 gtggtaactg ggtgaagatc ccacagattg gtcgcgtaat tattggcgat cgcgtggaga
    33241 tcggtgcctg cacaaccatc gatcgcggcg cgctggatga cactgttatt ggcaatggcg
    33301 tgatcattga taaccagtgc cagattgcac ataacgtcgt gattggcgac aatacggcgg
    33361 ttgccggtgg cgtcattatg gcgggcagcc tgaaaattgg tcgttactgc atgatcggcg
    33421 gagccagcgt aatcaacggg catatggaaa tatgcgacaa agtgacggtt acgggcatgg
    33481 gtatggtgat gcgtcccatc actgaaccag gcgtctattc ctcaggcatt ccgctgcaac
    33541 ccaacaaagt ctggcgcaaa accgctgcac tggtgatgaa cattgatgac atgagcaagc
    33601 gtctgaaatc gcttgagcgc aaggttaatc aacaagacta acgttccatc ttttgttcgc
    33661 caaactttac ggcctgtctc attcttacga ttgcggcagg ccgtgttatt attgtcgttt
    33721 cttatatttt gacaggaaga gtatcttgac tactaacact catactctgc agattgaaga
    33781 gattttagaa cttctgccgc accgtttccc gttcttactg gtggatcgcg tgctggattt
    33841 tgaagaaggt cgttttctgc gcgcagtaaa aaatgtctct gtcaatgagc cattcttcca
    33901 gggccatttc cctggaaaac cgattttccc gggtgtgctg attctggaag caatggcaca
    33961 ggcaacaggt attctggcgt ttaaaagcgt aggaaaactg gaaccgggtg agctgtacta
    34021 cttcgctggt attgacgaag cgcgcttcaa gcgcccggtc gtgcctggcg atcaaatgat
    34081 catggaagtc actttcgaaa aaacgcgccg cggcctgacc cgttttaaag gggttgctct
    34141 ggtcgatggt aaagtagttt gcgaagcaac gatgatgtgt gctcgtagcc gggaggcctg
    34201 atacgtgatt gataaatccg cctttgtgca tccaaccgcc attgtggaag agggcgcgtc
    34261 aattggcgcg aacgcacaca ttggtccttt ttgtatcgtt ggaccccatg tcgaaattgg
    34321 tgagggtacc gtactgaaat ctcacgttgt cgtgaatggt catactaaaa ttggccgcga
    34381 taatgagatt tatcagttcg cctccatcgg cgaagttaac caggatctga aatatgctgg
    34441 cgaaccgacc cgtgtggaaa tcggcgatcg taaccgcatt cgcgaaagcg tcaccattca
    34501 tcgtggcaca gtccagggcg gtggattgac gaaggtgggc agcgacaact tactgatgat
    34561 caacgcgcac attgcgcacg attgtacggt aggtaaccgc tgtattctcg ccaacaacgc
    34621 aacgctggcg ggtcacgtat cggttgacga cttcgcgatc atcggcggca tgaccgcagt
    34681 ccatcagttc tgcatcattg gtgcgcatgt gatggttggc ggctgctccg gtgtggcgca
    34741 ggacgtccct ccttatgtca ttgcgcaggg taaccacgcc acgccgttcg gtgtcaatat
    34801 cgaagggctg aagcgccgcg gattcagccg tgaggcgatt accgctatcc gcaatgcgta
    34861 taagctgatt tatcgtagcg gtaaaacgct cgatgaagtg aaaccggaaa ttgctgaact
    34921 ggcggaaaca tatccggaag tgaaagcctt taccgatttc tttgcacgct caacgcgcgg
    34981 tctgattcgt taatgactga acagcgtcca ttaacgattg ccctggtcgc cggagaaacc
    35041 tccggcgata tcctgggggc cggtttaatc cgcgctctga aagaacgtgt gcccaacgcc
    35101 cgctttgttg gtgttgccgg gccacgaatg caggctgaag gctgcgaagc ctggtacgaa
    35161 atggaagaac tggcggtgat gggcattgtt gaagtgctcg gtcgtctgcg tcgcttactg
    35221 catattcgtg ccgatctgac aaagcgtttt ggcgaactga agccagatgt ttttgttggt
    35281 attgatgcgc ctgacttcaa tattactctt gaaggtaacc tcaaaaagca gggtatcaaa
    35341 accattcatt acgtcagtcc gtccgtctgg gcgtggcgac agaaacgcgt tttcaaaata
    35401 ggcagagcca ccgatctggt gctcgcattt ctgcctttcg aaaaagcgtt ttatgacaaa
    35461 tacaatgtac cgtgccgctt tatcggtcat accatggctg atgccatgcc gttagatcca
    35521 gataaaaatg gtgcccgtga tgtgctgggg atcccttacg atgcccactg tctggcattg
    35581 ttgccgggaa gtcgtggcgc ggaagttgag atgcttagcg ccgatttcct gaaaacggcc
    35641 cagcttttgc gccagacgta ccctgatctg gagatcgtgg tgccgctggt gaatgccaaa
    35701 cgccgcgagc agtttgaacg catcaaagct gaagtcgcgc cagacctttc agttcatttg
    35761 ctggatggga tgggccgtga ggcgatggtc gccagcgatg cggcactact ggcgtcgggg
    35821 acggcagccc tggagtgtat gctggcgaaa tgcccgatgg tggtgggata tcgcatgaag
    35881 ccttttacct tctggttggc gaagcggctg gtgaaaactg attatgtctc gctgccaaat
    35941 ctgctggcgg gcagagagtt agtcaaagag ttattgcagg aagagtgtga gccgcaaaaa
    36001 ctggctgcgg cgctgttacc gctgctggcg aacgggaaaa ccagccacgc gatgcacgat
    36061 accttccgtg aactgcatca gcagatccgc tgcaatgccg atgagcaggc ggcacaagcc
    36121 gttctggagt tagcacaatg atcgaatttg tctatccgca cacgcagctg gttgcgggtg
    36181 tggatgaagt cggacgcggg ccgttagttg gcgcggtcgt caccgctgcg gtgatccttg
    36241 acccggcgcg cccgattgcc gggctgaatg attccaaaaa gctgagcgaa aaacgccgtc
    36301 tggcgctcta tgaagagatc aaagagaaag cgttgagctg gagtctgggc cgcgcggaac
    36361 cccacgaaat cgacgagctg aacattcttc atgcgaccat gctggcgatg cagcgtgccg
    36421 tcgctgggct gcatattgcg ccggaatatg tgttgattga tggtaaccgc tgcccgaaat
    36481 taccgatgcc tgcgatggct gtggtgaaag gcgatagccg cgtaccggaa atcagtgccg
    36541 cgtctatcct ggcgaaagtg acgcgtgacg ccgaaatggc ggcgctggat attgttttcc
    36601 cgcaatatgg ttttgcccaa cacaaagggt atccaaccgc ttttcatctg gaaaaactgg
    36661 ctgaatatgg cgcgactgaa catcatcgtc gcagctttgg gcctgtcaaa cgagcactgg
    36721 gacttgcgtc ctgattcttg tgtcgagatt aagtaaaccg gaatctgaag atgtctgaac
    36781 cacgtttcgt acacctgcgg gtgcacagcg actactcgat gatcgatggc ctggccaaaa
    36841 ccgcaccgtt ggtaaaaaag gcggcggcgt tgggtatgcc agcactggcg atcaccgatt
    36901 tcaccaacct ttgcggtctg gtgaagttct acggagcggg acatggcgca gggattaagc
    36961 ctatcgtcgg ggcagatttt aacgtccagt gcgacctgct gggtgatgag ttaacccacc
    37021 tgacggtact ggcggcgaac aataccggct atcagaatct gacattgctg atctcaaaag
    37081 cgtatcagcg cgggtacggt gccgccgggc cgatcatcga tcgcgactgg cttatcgaat
    37141 taaacgaagg gttgatcctt ctttccggcg ggcgcatggg cgacgtcgga cgcagtcttt
    37201 tgcgtggtaa cagcgcgctg gtagatgagt gtgtcgcgtt ttatgaagaa cacttcccgg
    37261 atcgctattt tctcgagctg atccgcaccg gcaggccgga tgaagaaagc tatctgcacg
    37321 cggcggtgga actggcggaa gcgcgcggtt tgcccgtcgt ggcgactaac gatgtgcgct
    37381 ttatcgacag cagcgacttt gacgcacacg aaatccgcgt cgcgattcac gacggcttta
    37441 ccctcgacga tcctaaacgc ccgcgtaact attcgccgca gcaatatatg cgtagcgaag
    37501 aggagatgtg cgagctgttt gccgacatcc cagaagccct tgccaacacc gttgagatcg
    37561 ccaaacgctg taacgtaact gtgcgtcttg gtgaatactt cctgccgcag ttcccgaccg
    37621 gggacatgag caccgaagat tatctggtca agcgtgcaaa agagggcctg gaagagcgtc
    37681 tggccttttt attccctgat gaggaagaac gtgttaagcg ccgctcggaa tatgacgaac
    37741 gtctggagac tgaacttcag gttatcaacc agatgggctt cccgggctac ttcctcatcg
    37801 ttatggaatt tatccagtgg tcgaaagata acggtgtacc ggtagggcca ggccgtggct
    37861 ccggcgcagg ctcactggtg gcctacgcgc tgaaaatcac cgacctcgat ccgctggaat
    37921 ttgacctgct gttcgaacgt ttccttaacc cggaacgtgt ctccatgcct gacttcgacg
    37981 ttgacttctg tatggagaaa cgcgatcagg ttatcgaaca cgtagcggac atgtacggtc
    38041 gtgatgcggt atcgcagatt atcaccttcg gtacgatggc ggcgaaagcg gtgatccgcg
    38101 acgtaggccg cgtgctgggg catccgtacg gctttgtcga tcgtatctcg aaactgatcc
    38161 cgcccgatcc ggggatgacg ctggcgaaag cgtttgaagc cgagccgcag ctgccggaaa
    38221 tctacgaagc ggatgaagaa gttaaggcgc tgatcgacat ggcgcgcaaa ctggaagggg
    38281 tcacccgtaa cgccggtaag cacgccggtg gggtggttat cgcgccgacc aaaattaccg
    38341 attttgcgcc gctttattgc gatgaagagg gcaaacatcc ggtcacccag tttgataaaa
    38401 gcgacgttga atacgccgga ctggtgaagt tcgacttcct tggtttgcgt acgctcacca
    38461 tcatcaactg ggcgctggag atgatcaaca agcggcgggc gaagaatggc gagccgccgc
    38521 tggatatcgc cgcgatcccg ctggatgaca agaaaagctt cgacatgctg caacgctcgg
    38581 aaaccacggc ggtattccag cttgaatcgc gcggcatgaa ggacctgatc aagcgtctgc
    38641 aacctgactg cttcgaagat atgatcgcac tggtggcact gttccgccct ggtccgttgc
    38701 agtcagggat ggtggataac tttattgacc gtaaacatgg tcgcgaagaa atctcctatc
    38761 cagacgtaca gtggcagcat gaaagcctga aaccggtact ggagccaacc tacggcatta
    38821 tcctgtatca ggaacaggtc atgcagattg cgcaggtgct ttctggttat accctcggtg
    38881 gcgcggatat gctgcgtcgt gcgatgggta agaaaaagcc ggaagagatg gcgaagcagc
    38941 gttctgtatt tgctgaaggt gcagaaaaga acggaatcaa cgccgaactg gcgatgaaaa
    39001 tcttcgacct ggtggagaaa ttcgctggtt acggatttaa caaatcgcac tctgcggcct
    39061 atgctttggt gtcatatcaa acattatggt tgaaagcgca ctatccggca gagtttatgg
    39121 cggcggtaat gaccgccgat atggacaaca ccgagaaggt ggtgggcctg gtggatgagt
    39181 gctggcggat ggggctgaaa atcctgccac cagatataaa ctccggtctt taccatttcc
    39241 acgtcaacga cgacggcgaa atcgtgtatg gtattggcgc gatcaaaggg gtaggtgaag
    39301 gtccgattga ggcgatcatc gaagcccgta ataaaggcgg ctacttccgc gaactgtttg
    39361 atctctgcgc tcgtaccgac accaaaaagt tgaaccggcg ggtgctggaa aaactgatca
    39421 tgtccggggc gtttgaccgt cttgggccac atcgcgcagc gctgatgaac tcgctgggcg
    39481 atgcgttaaa agcggcagat caacacgcga aagcggaagc tatcggtcag gccgatatgt
    39541 tcggcgtgct ggccgaagag ccggaacaaa ttgaacaatc ctacgccagc tgccaaccgt
    39601 ggccggagca ggtggtatta gatggggaac gtgaaacgtt aggtctgtac ctgacgggac
    39661 accctatcaa ccagtattta aaagagattg agcgttatgt cggaggcgta aggctgaaag
    39721 acatgcaccc gacagaacgt ggtaaagtca tcacggctgc ggggctcgtt gttgccgcgc
    39781 gggttatggt caccaagcgc ggcaatcgta tcggtatctg cacgctggat gaccgttccg
    39841 ggcggctgga agtgatgttg tttactgacg ccctggataa ataccagcaa ttgctggaaa
    39901 aagaccgcat acttatcgtc agcggacagg tcagctttga tgacttcagc ggtgggctta
    39961 aaatgaccgc tcgcgaagtg atggatattg acgaagcccg ggaaaaatat gctcgcgggc
    40021 ttgctatctc gctgacggac aggcaaattg atgaccagct tttaaaccga ctccgtcagt
    40081 ctctggaacc ccaccgctct gggacaattc cagtacatct ctactatcag agggcggatg
    40141 cacgcgcgcg gttgcgtttt ggcgcgacgt ggcgtgtctc tccgagcgat cgtttattaa
    40201 acgatctccg tggcctcatt ggttcggagc aggtggaact ggagtttgac taatacagga
    40261 atactatgag tctgaatttc cttgattttg aacagccgat tgcagagctg gaagcgaaaa
    40321 tcgattctct gactgcggtt agccgtcagg atgagaaact ggatattaac atcgatgaag
    40381 aagtgcatcg tctgcgtgaa aaaagcgtag aactgacgcg taaaatcttc gccgatctcg
    40441 gtgcatggca gattgcgcaa ctggcacgcc atccacagcg tccttatacc ctggattacg
    40501 ttcgcctggc atttgatgaa tttgacgaac tggctggcga ccgcgcgtat gcagacgata
    40561 aagctatcgt cggtggtatc gcccgtctcg atggtcgtcc ggtgatgatc attggtcatc
    40621 aaaaaggtcg tgaaaccaaa gaaaaaattc gccgtaactt tggtatgcca gcgccagaag
    40681 gttaccgcaa agctctgcgc ctgatgcaaa tggctgaacg ctttaagatg ccaatcatca
    40741 cctttatcga caccccgggg gcttatccgg gcgtgggcgc agaagaacgc ggtcagtctg
    40801 aagctattgc acgcaacctg cgtgaaatgt ctcgcctcgg tgtaccggta gtttgtacgg
    40861 ttatcggtga aggtggttct ggcggcgcgc tggcgattgg cgtgggcgat aaagtgaata
    40921 tgctgcaata cagcacctat tccgttatct cgccggaagg ttgtgcgtcc attctgtgga
    40981 agagcgctga caaagcgccg ctggcagctg aagcgatggg tatcatcgct ccgcgtctga
    41041 aagaactaaa actgatcgac tccatcatcc cggaaccgct gggcggcgct caccgtaacc
    41101 cggaagcgat ggcggcatcg ttgaaagcgc aactgctggc ggatctggcc gatctcgatg
    41161 tgttaagcac tgaagattta aaaaatcgtc gttatcagcg cctgatgagc tacggttacg
    41221 cgtaattcgc aaaagttctg aaaaagggtc acttcggtgg ccctttttta tcgccacggt
    41281 ttgagcaggc tatgattaag gaaggatttt ccaggaggaa cacatgaaca tcattgccat
    41341 tatgggaccg catggcgtct tttataaaga tgagcccatc aaagaactgg agtcggcgct
    41401 ggtggcgcaa ggctttcaga ttatctggcc acaaaacagc gttgatttgc tgaaatttat
    41461 cgaacataac cctcgaattt gcggcgtgat ttttgactgg gatgagtaca gtctcgattt
    41521 atgtagcgat atcaatcaac taaatgaata tcttccgctt tatgccttca tcaacaccca
    41581 ctcgacgatg gatgtcagcg tgcaggatat gcggatggcg ctctggtttt ttgaatatgc
    41641 gcttgggcag gcggaagata tcgccattcg tatgcgtcag tacaccaacg aatatcttga
    41701 taacattaca ccgccgttca cgaaagcctt gtttacctac gtcaaagagc ggaagtacac
    41761 cttttgtacg ccgggtcata tgggcggcac cgcgtatcaa aaaagcccgg ttggctgtct
    41821 gttttatgat tttttcggcg ggaatactct taaggctgat gtctctattt cggtcaccga
    41881 gcttggttcg ttgctcgacc acaccgggcc acacctggaa gcggaagaat acatcgcgcg
    41941 gacttttggc gcggaacaga gttatatcgt taccaacgga acatcgacgt cgaacaaaat
    42001 tgtgggtatg tacgccgcgc catccggcag cacgctgttg atcgaccgca actgccataa
    42061 atcgctggcg catctgttga tgatgagcga tgtagtgcca gtctggctga aaccgacgcg
    42121 taatgcgttg gggattctgg gtgggatccc gcgccgtgaa tttactcgtg acagcatcga
    42181 agagaaagtc gctgccacca cgcaggcaca atggccggtt catgcggtga ttaccaactc
    42241 aacctatgat ggcttgctct acaacaccga ctggatcaaa cagacgctgg atgtcccgtc
    42301 gatccacttc gattccgcct gggtgccgta cacccatttt catccgatct accaggggaa
    42361 aagtggtatg agcggcgagc gtgttgcggg aaaagtgatc ttcgaaacgc aatcgaccca
    42421 caaaatgctg gcggcgttat cgcaggcttc gctgatccac attaaaggtg agtatgacga
    42481 agaggcgttt aacgaagcct ttatgatgca taccaccacc tcgcccagtt atcccattgt
    42541 tgcttcggtt gagacggcag cagcgatgct gcgtggtaat ccgggtaaac ggctgattaa
    42601 ccgttcagta gaacgagctc tgcattttcg caaagaggtc cagcggctgc gggaagagtc
    42661 tgacggttgg tttttcgata tctggcaacc gccgcaggtg gatgaagccg aatgctggcc
    42721 cgttgcgcct ggcgaacaat ggcacggctt taacgatgcg gatgccgatc atatgttcct
    42781 cgatccggtt aaagtcacta ttctgacgcc ggggatggac gagcagggca atatgagcga
    42841 ggaggggatc ccggcggcgc tggtggcgaa gttcctcgat gagcgtggga tcgtggtaga
    42901 gaaaaccggc ccatataacc tgctgtttct ctttagtatt ggcatcgata aaaccaaagc
    42961 aatgggatta ttgcgtgggt tgacggaatt taaacgctct tacgatctca atctgcgaat
    43021 caaaaatatg cttccggatc tgtacgcaga agatcccgat ttctaccgca atatgcgtat
    43081 tcaggatctg gcgcaaggga tccataagct aattcgtaaa cacgatcttc ccggtttgat
    43141 gttgcgggca ttcgatactt tgccggaaat gatcatgacg ccacatcagg catggcagag
    43201 acagattaaa ggtgaagtag aaaccattgc gctggaacaa ctggtcggta gagtatcggc
    43261 aaatatgatc ctgccttatc ctcctggcgt accgctgttg atgcctggag aaatgctgac
    43321 caaagagagc cgcacagtac tcgattttct actgatgctt tgttccgtag ggcaacatta
    43381 ccccggtttt gaaacggata ttcacggcgc gaaacaggac gaagacggcg tttaccgcgt
    43441 acgagtccta aaaatggcgg gataacttgc cagagcggct tccgggcgag taacgagctg
    43501 ttaacaaata aaggagacgt tatgctgggt ttaaaacagg ttcaccatat tgcgattatt
    43561 gcgacggatt atgcggtgag caaagctttc tactgcgata ttcttggttt cacgctgcaa
    43621 agcgaagtct atcgcgaagc gcgcgactcg tggaaagggg atttggcgct taatgggcaa
    43681 tatgtgattg agcttttctc atttccgttc ccgccggagc gccccagccg accggaagct
    43741 tgcggcctgc gtcatctggc ttttagcgtt gatgatatcg atgcggcagt ggcgcacctt
    43801 gaaagccata acgtgaagtg tgaagccatc cgtgtcgatc catacacgca aaaacgcttc
    43861 acgttcttta acgatccgga cgggctgccg ttggaactgt atgagtggta aggcttgtca
    43921 tcgcggcatt tgcccggtaa cgtgccgggc attgctactg taaaatcgca ccatcatgac
    43981 actcacgctc aatagacaac ttctcacctc acgccagatt ctggtggcct ttagcggcgg
    44041 gcttgactcc accgttctgc tgcatcagtt ggtgcagtgg cggacggaaa atccgggtgt
    44101 cgctctgcgc gctattcatg tgcatcacgg tttaagtgcc aatgccgatg cctgggttac
    44161 gcattgcgaa aacgtctgcc aacagtggca ggtgccgctg gtggtcgaac gcgtacaact
    44221 tgcgcaagag gggctgggga ttgaggccca ggcgcggcag gcgcgttatc aggcatttgc
    44281 ccgcaccttg ttgcccggtg aagtgctggt caccgcgcaa catctcgacg atcaatgtga
    44341 aacttttctg ctggcgctaa aacgcggtag cggccctgcc gggctttcgg ctatggcgga
    44401 agtttcggag tttgccggaa cacggcttat ccgcccgttg cttgcccgca cgcgggggga
    44461 acttgcgcag tgggcattgg cgcatggttt acgctggatt gaagacgaaa gtaatcagga
    44521 cgactcatac gatcgtaact ttctgcgcct gcgcgtagtg ccgttattgc agcagcgttg
    44581 gccgcatttt gccgaaacaa cggcccgcag cgccgcactt tgtgctgaac aagagagcct
    44641 gttggatgaa ctgctggcgg atgatttagc acactgtcaa tcgccgcagg ggacgctgca
    44701 gattgcgcca atgctggcga tgagtgatgc ccgtcgcgcg gcgattatcc gccgctggct
    44761 ggcagggcag aatgcaccga tgccttcccg cgacgcgttg gtgaggatct ggcaggaagt
    44821 ggcgctggcg cgggaagatg cctccccctg tttacgtttg ggcgcgtttg aaatccgacg
    44881 ctatcagtcg caactgtggt ggattaaatc cgtcaccggg caaagcgaaa acattgtgcc
    44941 gtggcagacg tggcttcaac cgctggaatt accggcgggg ctgggaagtg tacagcttaa
    45001 tgcgggaggc gatattcgcc ctccgcgtgc agacgaagcg gtcagcgtgc gtttcaaagc
    45061 gccaggattg ctgcatattg tcgggcgtaa cggcggacgt aagctaaaga aaatctggca
    45121 agagctgggc gtgccgccgt ggctacgtga caccacgcca ctgctgtttt atggcgaaac
    45181 gctgattgcg gcggcagggg tatttgtgac gcaagaaggt gtggctgaag gtgagaatgg
    45241 cataagtttt gtctggcagc aaaacgctta gttaagtgaa ggccggatat gacgcattaa
    45301 gcgtcgcatc cggcgaaagt caatcaggac tcgctcacca ccaccgtacc gatttccggg
    45361 tggctaaagc tggtaatttt atccagacgt agctcacggg tttcgccagc ggcctcgacg
    45421 accagatact ccacattttt gcgggagact aaatcgctgg cttttgcctg caatttttcg
    45481 ccatctttca gctcaagtgt cagcattaaa tgatgctggc aggcgagctc aagattatcg
    45541 taatcatcac aattgattgg ttgatacgta tcattcattg acataatcgc tcaccagtaa
    45601 gtttgccgca gcgtatgctg ctttttccct gacagcctca gaaagggcgt cgtcggcagc
    45661 catttcattc agcactttca aaacgcagcc cagcgcgtcc ggaacgtatc ctaagtctcc
    45721 gctggcgatt tccgcgtacc gcttgcgtat taactcacaa tatttttcca catgccctcc
    45781 tgtcagcact ctgacttaac cgtggatgca agtctaagcc tacgaagata aactctgttt
    45841 cgcaaggtga ctataccaca ctcatttctg caatatcagc gccgcaactg cacgtattcc
    45901 gttacaatgg cctcctgatt cgaaaggagt tttcttatgg cgcttaaagc gacaatttat
    45961 aaagcgacgg ttaatgtggc cgatctcgac cgcaaccagt ttctcgatgc ctctctgacg
    46021 ctggcgcgcc acccttcaga aacccaggag cgtatgatgc tgcgcttgct ggcgtggctg
    46081 aaatatgctg atgaacgtct gcaatttacc cgtggtttgt gtgccgatga tgagccggaa
    46141 gcgtggctgc gtaacgatca cctgggcatt gatttgtgga ttgagctggg gctgccggat
    46201 gagcggcgga ttaagaaagc ctgcacccag gccgcagaag tggcgctgtt tacctataat
    46261 agtcgggcgg cgcaaatctg gtggcagcaa aatcagagca aatgtgtgca gtttgccaat
    46321 ctttccgtct ggtatctgga cgatgaacaa ctggcgaaag taagcgcctt tgccgatcgt
    46381 accatgacgc tgcaggcaac gattcaggat ggcgtgatct ggttatcgga tgataagaat
    46441 aatctggaag tgaacttaac cgcctggcaa caaccttcat gattgtgatt tcccgacatg
    46501 ttgctattcc cgatggtgag cttgagatca ccgccattcg tgcgcagggc gcgggcgggc
    46561 agcatgttaa taagacctca acggctattc atctgcgttt tgacattcgg gcgtccagcc
    46621 tgccagagta ttacaaagag cgtctgctcg ccgccagcca tcatttgatc agcagtgatg
    46681 gcgtgattgt cattaaggca caggaatacc gcagtcagga actgaaccgc gaagccgctc
    46741 tggcccggct ggtggctgtg attaaagaat taacaacaga acaaaaagcc cgacgaccca
    46801 cgcggcccac ccgtgcatcg aaagagcgca ggctggcatc gaaagcacaa aaatcaagcg
    46861 tgaaggcgat gcgcggcaaa gtgcgcagcg gtcgggaata aaaagaagga atggatggtg
    46921 aaaaaagcga tagtgacagc gatggctgta atcagcctct ttactctgat gggttgtaat
    46981 aatcgggccg aagtcgatac gctttctccg gcgcaggcag ccgaactgaa accgatgccg
    47041 caaagttggc gcggcgtgct gccgtgtgcc gattgcgaag gaatcgaaac ctctctgttc
    47101 ctcgaaaaag acggaacatg ggtgatgaat gagcgttatc tcggtgctcg tgaagaacct
    47161 tcctccttcg cttcctacgg tacatgggcg cgaaccgctg acaagctggt attaaccgac
    47221 agcaaaggtg aaaagtcata ttatcgggcg aaaggcgatg cgctggagat gctcgatcgt
    47281 gaaggcaatc cgattgaatc gcagttcaac tatacgctgg aaccggcaca atccagttta
    47341 cccatgacgc cgatgaccct gcggggcatg tatttttata tggctgatgc ggcgaccttc
    47401 actgattgcg tgaccggaaa acgtttcatg gtagcgaata acgcagagct ggagcgtggc
    47461 tacctggctg cgcgtggtca cagtgaaaaa ccggtgttac tgtcagtaga aggtcacttt
    47521 acgcttgagg ctaatccgga taccggtgcg ccaaccaaag tattagcgcc cgatacggca
    47581 ggtaaatttt acccaaacca ggattgcagt agtttggggc agtaacccgt cttgagacag
    47641 aaacaaacgc aaaacaggcc agaaggatat atttcaacat tttgaatttg cacgtttttt
    47701 gtaggccgga taaggcgttt acgccgcatc cggcaatggt gctcaacgcc tgatgcgacg
    47761 ctggcgcgtc ttatcatgcc tacaaccccc ctcatacctt aaggcggata aggcatttac
    47821 tttatcaccg ggtcaaacca acaaagcgac ccgccttgat ataaatccct ggcttcaggt
    47881 gcccaacata ctgtaactgc gtttctggct taaacgcaga gacatcacca atacgcatat
    47941 gcatcagatc ggcagggcta atccagcccg attgcgccag cgtaagcgga tgcccggctt
    48001 tggcaaatgc atcagtgaca aattccgaac aaaaccacga ctttttgtct ccttcgccca
    48061 cactgcttaa ctgcgctttc gccaggccgc tgacgcactg ttggcgaaaa tctgcggaaa
    48121 aaggattcag tgagcacatc tggcgggtca ccataaatgg aatgaactcg acaatgccgc
    48181 gatagttata cccgctatct ttaattttgt tggcgaacgc ggtgatttct gtggcttgtt
    48241 gcggggtaag atccgggact cgtaagacga aaagcttatc gctatgcttc atcgcttttt
    48301 taagggaaac aatctggacg ccagcgcctg tcgcttctgc aacgttatta tcacccagat
    48361 agattgcaac gtgactcaca gaggaagtgc tgaaaacgcg gatcccaaat gaggttaccc
    48421 ccaggcttga ggagaacagc aaatcgccgg gtttgagatc aggtaccgtt atttctttta
    48481 ttgattgttc ggtgaaagag ctttgatgct ggaatttgac agcccatgtt ttcgcctcgg
    48541 catctactgc tgtggcagag gagtctggct ggctgatatc aacggtacag gccgaaagta
    48601 aaagaaagca ggggagaagc aggcggcagt acgcctttgg tttatccatt ttatacaatc
    48661 catgtaaaaa agggccctga aattcaggac cctttctggc atcagccttt aatctgtttc
    48721 accagatatt cgacgatgtc accagtctta attaactgtt tctcgccgtt gcgacgatat
    48781 ttatattcga tatcgtcgtt gtcgaggtta cggtcgccca gcacaatagt gtgcggaata
    48841 ccgatcagtt ccatatcggc aaacatcacg cccggacgct ctttgcggtc atccagcagc
    48901 acttcgatac cttgtgcgcg cagttcgctg tacagtttct cagcaagctc ttgtacgcgg
    48961 aaggatttgt gcatgttcat cggcagaatc gccacctgga acggcgcgat agcgtcaggc
    49021 catacgatgc cgcgttcgtc gtagttctgc tcaatcgccg cagctactac acgcgttacc
    49081 ccgataccgt agcaacccat agtcaggatt tggttacggc catcttcacc ctgtacggag
    49141 gctttcagtg cttcggagta cttggtaccc agctggaaga tgtgaccaac ttcgatacca
    49201 cgtttgatca gcagcgtacc ctgaccatcc gggcttggat cgcccgccac cacgttgcgg
    49261 atatcagcaa tttccggggt agcgacatcg cgatcccagt tgataccgaa gtagtgttta
    49321 ccatcgatgt tagcaccagc agcgaaatca ctcatcgccg caacggtacg gtcaatcacc
    49381 accggaatcg gcatgtttac cggacccagt gaacccggac cggctttaac cacggcacga
    49441 atttcttctt cagtcgcaaa agtcagcggg ctggcaacct gcggcagttt ttctgcttta
    49501 acttcgttca gctcgtgatc accgcgcacc agcagcgcaa ccagcgggaa gctactgcct
    49561 tcaaccgctt taaccagcag agtcttaacc gttttctcaa tcggcagatt gaactgttca
    49621 accagttccg cgatggtttt cgcgttcggc gtatcaacca gcgtcatttc ctgggtagca
    49681 gcagcgcgcg gttctttcgg cgcgatagct tctgccagtt caatgttcgc tgcatagtca
    49741 gaggtgtcgg agaagaccac atcgtcttca ccgctctgcg ccagcacctg gaattcgtga
    49801 gaggcgctgc cgccgataga accggtgtcg gcttgcacgg cgcggaaatc cagccccatg
    49861 cggctgaaga ttttgctgta ggccgcatac attgcatcgt aggtttcctg cagggattcc
    49921 tgagaagtat ggaaagagta agcatctttc atcaggaatt cgcgggaacg catgacgccg
    49981 aaacgcggac gcacttcgtc gcggaacttg gtctggatct gatagaagtt cagcggcagc
    50041 tgtttgtaag agctaagctc gttacgaatc aggtcagtga taacttcttc atgagttggg
    50101 ccgagtacga acggacgctc gccacggtca acaaaacgca gcagttccgg accgtactgt
    50161 tcccaacgac cactctcttg ccacaaatcg gctggctgaa ccaccggcat cgacacctcg
    50221 attgcaccgg cgttgttcat ctcttcacgc acgatgtttt cgactttttt cagaacgcgc
    50281 acgccggtcg gcagccaggt atataacccg gaggccagct tgcggatcat cccggcgcgc
    50341 agcatcagct gatggctgat cacctcggcg tcggcaggtg tctccttgag agtggagagc
    50401 aggtattggc tagtacgcat gttgttacgg ttccagttgg aaggtagaac aggctcaagg
    50461 cgagcctggg acaaaaaaag tgatttagtt taccagtgca aaagaaatgt caaaagagaa
    50521 gggcgtgaat ttaacgcggt tccagcgcaa agacttcaaa acctgcgtcg gtgacgcgcc
    50581 agcgaacgtt aaaatcatgt agccagacgg cataggtttt gcccgtttcc tcacctttac
    50641 gataggccgg gcgcgggtcc tgcgccagta cttcgcggat aaacagcgtt aactgcggat
    50701 aacgcttctc cagcgtcaaa agctgctttt cgacctctgc ggtaaaactc accgccatct
    50761 ctgccgctgg cgcactttgc gcatagctgg cactggcatc gggaagcgat tcggcaaagg
    50821 ggagatacgg tttgatatcc actaccggcg taccatcgac cagatccaga ctgccgagct
    50881 ttagaatcac gctgtcttta tggcaaacaa cctctttcag ctctaccagc gacataccaa
    50941 ttgggttagg gcggaaagta gagcgtgttg cgaaaacccc cattctggcg ttaccgccga
    51001 ggcgcggtgg acgcacagtc ggacgccagc cgccttccat cgtttgatga aagacgaaaa
    51061 ggatccataa atggctgaac gcttccaggc ctcgaacggc gtcggcctgg ttgtagggag
    51121 caatgagatg cagttcgccg ttggcgcttt ttaccagacc tggctggcgc ggaacggcga
    51181 atttttcttt atagggcgag cgaataacgc ctatttgctc gaactggaaa ctgctcattt
    51241 cgccgtaatg ttaagcgcag agccgataca tacagcctga cgatagcagc ccggcgtacc
    51301 gctggtgact tcgcagctat gcagtaatac agcattggct ttcattttag aggcgttgat
    51361 ttgcatccgc ttacgtgcgg ttggaatgct cggcggagag tcctgattag aggcctggca
    51421 agagtcgcca ctgacttcac cgagatcgcg gaacggtttg ccgactaatt cttctgcatt
    51481 ggtataaatt cggaccggcg tggcgcgcgg cgctttcggt tttgcaggct ccgctttcgg
    51541 ctggggtgca gtgctttgaa cgggttcgac aggggatctg cttaacatgg aacagccgct
    51601 tagcatgagt gctactaaac agatcggtaa agcacgcata gtatttcctc aatgtatgat
    51661 caaaacgtca atattgaatc aggagcttgt aaaaatgaca agacgggcaa gcgcccgtcc
    51721 tgaatgatat tacaaattgt ggaaacagcc taaaaattac cagcctttaa cagcgccgcc
    51781 gttaaacact ttgtttgctg cttcgtaaac ttcgtcagac tgataagcct ggacgaattt
    51841 cttcacgttc tcggcgtctt tgttatcttc acgcgttacg atcaggttta cgtacgggga
    51901 gtctttatct tcgacaaaga taccgtcttt cgccggagtc aggccaatct ggctggcata
    51961 ggtggtattg ataactgcca gagcgatttg cgcgtcgtcc agagaacgcg gcagctgcgg
    52021 tgcttccagc tcaacaattt tcagattttt ggggttctca acaacatcaa gaacggtcgg
    52081 cagcaggcca acgccatctt tcagtttgat caagcccact ttttgcagca gcagcagtga
    52141 acgaccaagg ttagttgggt cgtttggcac ggcaacctgc gcaccatcct gcagttcagc
    52201 cagtgatttg attttcttgg agtaacctgc aatcggataa acaaaagtgt tgcctactgc
    52261 gaccagtttg tagccacgat ctttcagttg ctgatcaagg tacggtttat gctggaaggc
    52321 gttggcgtcg atatcgcctt tgctcaatgc ttcgtttggc agaacatagt cgttgaaggt
    52381 taccagctca acgtccaggc catatttgtc tttcgcaact ttctgcgcga cttctgcaac
    52441 ctgctgttcg gcaccaacaa tcacgccgac tttaatgtgg tttggatctt tttcatcctg
    52501 accgcagcct accagtgcca gtgatccaat cagggctccc actgccgcaa aggttttgaa
    52561 tttgaacgcc ataccttatt ccttcttcaa ttatttatgt tgtgttgaac gttacttgcg
    52621 agtgacagcc cggacgatgc ggtcgcctgc gaactgaatt aaataaacca gaatgaccag
    52681 caataccagt accgtattca tcaccgtcgc gttatagccg atgtagccat actgatagcc
    52741 aatctgacct aaaccaccgg caccgactgc accacccatc gcggaataac cgaccagggt
    52801 aatcagggtg atagttgccg cattcaccag acccggcaac gcttccggta acagcacttt
    52861 acggacgatc tgcattggcg tggcacccat tgcgcgggaa gcttcaatta acccggttgg
    52921 gatctccagc agagcgttct cgaccatacg ggcaataaac ggtgcagcgc caacggtcag
    52981 cggaacaatc gctgcctgca aaccaatcga tgtaccgaca ataacgcggg taaacggaat
    53041 catccatacc agcaagataa tgaacgggat ggaacggaaa atgttcacaa tcgcagaaac
    53101 ggtacggtaa agcttcgcgt tagcaataat ttgccccgga cgcgtgacat aaagcaggac
    53161 gccgaccggc agaccaatca caaacccaaa aaatccggat acgaaggtca ttgccagcgt
    53221 ttcccatacg ccacgaacca gcagccacat catcggctca gacataaccc agtacctcta
    53281 cttttacatg gtgttcctgc agccaggcaa tggcggcttg cgtatcttgt tgtgtgccgt
    53341 gcatttcagt cagcatgatg ccgaacttca cgccaccggc gtaatccatc tgcgcgctaa
    53401 taatgttgtt gttgacgttg aaacgacgcg cggtttcaga aagcagtggg gcatcgaccg
    53461 attgaccggt aaactccaga cgcagcatcg ggacgcagtc agtgaatggc tccgcttgca
    53521 gacgttcctg gtaatcttcc gggatatcca gatgcagggt tgactgaata aacttctgcg
    53581 ccagaggcgt tttcggatgc gagaacactt cacttaccgt gtcctgctcg atcagttcgc
    53641 cattactgat aaccgccacg caatcacaaa tgcgcttcac aacgtccatt tcgtgggtga
    53701 tcaacagaat cgtcaaaccc agacggcggt taatgtcttt cagcagttcg agaatagaac
    53761 gtgtcgttgc cgggtctagc gcgctggtgg cttcatcaca cagcaatact ttgggattgc
    53821 tggctaatgc acgggcaatc gccacacgtt gtttctgccc accggaaaga ttcgacgggt
    53881 agctatcatg cttatcgcca agaccaacca atgacagcaa ttccgtcacg cgacgtttga
    53941 tctcgtcttt cggtgtattg tccagctcca gcggcagagc cacgttgcca aaaacagtac
    54001 gcgaagagag caggttaaaa tgctggaaaa tcataccaat ctggcgacga gctttggtca
    54061 actcggattc tgacagcgtg gtcagttcct ggccatcgac cagcacacta ccctcggttg
    54121 ggcgctccag caggtttaca caacgtataa gcgtactctt acccgcgcct gaggcaccga
    54181 taacgccata aatttgccca gctggcacat gcaggctgac gttgttcaac gcctggatgg
    54241 tgcgggtgcc ctggtggaac actttggtga tattcgaaag ttttatcatt gattatttat
    54301 tatcgtcatt aagttagtcg tggcatctcg aatgcctgaa acgggcaacg ccgtcaatga
    54361 aatggatgtt aaggcatcca gacgtctaaa tcaatcaggt ttatgcgaag agcactttct
    54421 tgcaggtcga aacatgcgat actagcgtca catgccttat taaggagcta taaaaggtgg
    54481 cgaagagcgt acccgcaatt tttcttgacc gtgatggcac cattaatgtc gatcacggct
    54541 atgtccatga gatcgacaac tttgaattta tcgacggtgt tattgacgcc atgcgcgagc
    54601 taaaaaaaat gggctttgcg ctggtggtag taaccaacca gtctggcatt gctcgcggta
    54661 aatttaccga agcacagttt gaaacgctga ccgagtggat ggactggtcg ctggcggacc
    54721 gagatgtcga tctggatggt atctattatt gcccgcatca tccgcagggt agtgttgaag
    54781 agtttcgcca ggtctgcgat tgccgcaaac cacatccggg gatgcttttg tcagcacgcg
    54841 attatttgca tattgatatg gccgcttctt atatggtggg cgataaatta gaagatatgc
    54901 aggcagcggt tgcggcgaac gtgggaacaa aagtgctggt gcgtacgggt aaacctatta
    54961 cacctgaagc agaaaacgcg gcagattggg tgttaaatag cctggcagac ctgccgcaag
    55021 cgataaaaaa gcagcaaaaa ccggcgtaat gattaaaaga tgagcggttg aaataaaaat
    55081 gcatttttcc gcttgtcttc ctgagccgac tccctataat gcgcctccat cgacacggcg
    55141 gatgtgaatc acttcacaca aacagtcggt tcggttgaag agaaaaaatc ctgaaattca
    55201 gggttgactc tgaaagagga aagcgtaata tacgccacct cgcgacagtg agctgaaagc
//
LOCUS       DAPGOF010000007        52661 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.007, whole genome shotgun sequence.
ACCESSION   DAPGOF010000007 DAPGOF010000000
VERSION     DAPGOF010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 52661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52661
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.007"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            129..509
                     /locus_tag="RHV13_000351"
     CDS             129..509
                     /locus_tag="RHV13_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HDV1239918.1"
                     /translation="MSKEYMNDGSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(552..1502)
                     /gene="corA"
                     /locus_tag="RHV13_000352"
     CDS             complement(552..1502)
                     /gene="corA"
                     /locus_tag="RHV13_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HDV1239919.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            1872..2636
                     /locus_tag="RHV13_000353"
     CDS             1872..2636
                     /locus_tag="RHV13_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HDV1239920.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(2782..4944)
                     /gene="uvrD"
                     /locus_tag="RHV13_000354"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(2782..4944)
                     /gene="uvrD"
                     /locus_tag="RHV13_000354"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HDV1239921.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(5028..5744)
                     /gene="yigB"
                     /locus_tag="RHV13_000355"
     CDS             complement(5028..5744)
                     /gene="yigB"
                     /locus_tag="RHV13_000355"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HDV1239922.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(5744..6640)
                     /gene="xerC"
                     /locus_tag="RHV13_000356"
     CDS             complement(5744..6640)
                     /gene="xerC"
                     /locus_tag="RHV13_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDV1239923.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(6637..7344)
                     /locus_tag="RHV13_000357"
     CDS             complement(6637..7344)
                     /locus_tag="RHV13_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HDV1239924.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(7341..8165)
                     /gene="dapF"
                     /locus_tag="RHV13_000358"
     CDS             complement(7341..8165)
                     /gene="dapF"
                     /locus_tag="RHV13_000358"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDV1239925.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(8202..8405)
                     /locus_tag="RHV13_000359"
     CDS             complement(8202..8405)
                     /locus_tag="RHV13_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDV1239926.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            8564..8706
                     /locus_tag="RHV13_000360"
                     /pseudo
     CDS             8564..8706
                     /locus_tag="RHV13_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            8868..9188
                     /gene="cyaY"
                     /locus_tag="RHV13_000361"
     CDS             8868..9188
                     /gene="cyaY"
                     /locus_tag="RHV13_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HDV1239927.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEKAATQQAGET
                     VSFR"
     gene            complement(9228..11774)
                     /gene="cyaA"
                     /locus_tag="RHV13_000362"
     CDS             complement(9228..11774)
                     /gene="cyaA"
                     /locus_tag="RHV13_000362"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HDV1239928.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            12161..13102
                     /gene="hemC"
                     /locus_tag="RHV13_000363"
     CDS             12161..13102
                     /gene="hemC"
                     /locus_tag="RHV13_000363"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HDV1239929.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDARTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            13099..13839
                     /gene="hemD"
                     /locus_tag="RHV13_000364"
     CDS             13099..13839
                     /gene="hemD"
                     /locus_tag="RHV13_000364"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HDV1239930.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGQQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPTYFAIGRTTALALHTISGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            13861..15054
                     /gene="hemX"
                     /locus_tag="RHV13_000365"
     CDS             13861..15054
                     /gene="hemX"
                     /locus_tag="RHV13_000365"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDV1239931.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQQETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPQAAPQADTPAA
                     APQGE"
     gene            15057..16253
                     /gene="hemY"
                     /locus_tag="RHV13_000366"
     CDS             15057..16253
                     /gene="hemY"
                     /locus_tag="RHV13_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HDV1239932.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            16932..18587
                     /gene="aslA"
                     /locus_tag="RHV13_000367"
     CDS             16932..18587
                     /gene="aslA"
                     /locus_tag="RHV13_000367"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase AslA"
                     /protein_id="HDV1239933.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYLVKPATTIADNMMPVMQHPAQDKETQQKLAELEKKTGKKPNVVVFLLDDVGWMDVG
                     FNGGGVAVGNPTPDIDAVASQGLILTSAYSQPSSSPTRATILTGQYSIHHGILMPPMY
                     GQPGGLQGLTTLPQLLHDQGYVTQAIGKWHMGENKESQPQNVGFDDFRGFNSVSDMYT
                     EWRDVHVNPEVALSPDRSEYIKQLPFSKDDVHAVRGGEQQAIADITPKYMEDLDQRWM
                     EYGVKILDKMAKSDKPFFLYYGTRGCHFDNYPNAKYAGSSPARTSYGDCMVEMNDVFA
                     NLYKALEKNGQLDNTLIVFTSDNGPEAEVPPHGRTPFRGAKGSTWEGGVRVPTFVYWK
                     GMIQPRKSDGIVDLADLFPTALDLAGHPGAKVANLVPKTTFIDGVDQTSFFLGTNGQS
                     NRKAEHYFLNGKLAAVRMDEFKYHVLIQQPYAYTQSGYQGGFTGTVMQTAGSSVFNLY
                     TDPQESDSIGVRHIPMGVPLQTEMHAYMEILKKYPPRAQIKSD"
     gene            complement(18755..19990)
                     /locus_tag="RHV13_000368"
     CDS             complement(18755..19990)
                     /locus_tag="RHV13_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HDV1239934.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQTLYREKPVTH
                     MDDDTLEAYVRHYIAASETQNEVAFTWQGGEPTLLGLDFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLTDETAARDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVSK"
     gene            complement(20137..20213)
                     /locus_tag="RHV13_000369"
     tRNA            complement(20137..20213)
                     /locus_tag="RHV13_000369"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20177..20179),aa:Pro,seq:tgg)
     gene            complement(20256..20342)
                     /locus_tag="RHV13_000370"
     tRNA            complement(20256..20342)
                     /locus_tag="RHV13_000370"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20306..20308),aa:Leu,seq:cag)
     gene            complement(20363..20438)
                     /locus_tag="RHV13_000371"
     tRNA            complement(20363..20438)
                     /locus_tag="RHV13_000371"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20403..20405),aa:His,seq:gtg)
     gene            complement(20497..20573)
                     /locus_tag="RHV13_000372"
     tRNA            complement(20497..20573)
                     /locus_tag="RHV13_000372"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20537..20539),aa:Arg,seq:ccg)
     gene            complement(20676..22061)
                     /locus_tag="RHV13_000373"
     CDS             complement(20676..22061)
                     /locus_tag="RHV13_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDV1239935.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(22252..22992)
                     /gene="rffM"
                     /locus_tag="RHV13_000374"
     CDS             complement(22252..22992)
                     /gene="rffM"
                     /locus_tag="RHV13_000374"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HDV1239936.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(22995..24347)
                     /gene="wzyE"
                     /locus_tag="RHV13_000375"
     CDS             complement(22995..24347)
                     /gene="wzyE"
                     /locus_tag="RHV13_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HDV1239937.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(24344..25423)
                     /gene="rffT"
                     /locus_tag="RHV13_000376"
                     /gene_synonym="wecF"
     CDS             complement(24344..25423)
                     /gene="rffT"
                     /locus_tag="RHV13_000376"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HDV1239938.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDNCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPKLWLALLSGGIKSR
                     QFFWHIWGADLYELSSGLRYKLFYSLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQVLSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(25420..26670)
                     /gene="wzxE"
                     /locus_tag="RHV13_000377"
     CDS             complement(25420..26670)
                     /gene="wzxE"
                     /locus_tag="RHV13_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HDV1239939.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(26672..27802)
                     /gene="rffA"
                     /locus_tag="RHV13_000378"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(26672..27802)
                     /gene="rffA"
                     /locus_tag="RHV13_000378"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HDV1239940.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMETIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDSYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(27807..28481)
                     /gene="rffC"
                     /locus_tag="RHV13_000379"
                     /gene_synonym="wecD"
     CDS             complement(27807..28481)
                     /gene="rffC"
                     /locus_tag="RHV13_000379"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HDV1239941.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEIDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASSRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(28459..29340)
                     /gene="rfbA"
                     /locus_tag="RHV13_000380"
     CDS             complement(28459..29340)
                     /gene="rfbA"
                     /locus_tag="RHV13_000380"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDV1239942.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(29359..30426)
                     /gene="rffG"
                     /locus_tag="RHV13_000381"
     CDS             complement(29359..30426)
                     /gene="rffG"
                     /locus_tag="RHV13_000381"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDV1239943.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNALTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKSLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIAHELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(30426..31688)
                     /gene="wecC"
                     /locus_tag="RHV13_000382"
     CDS             complement(30426..31688)
                     /gene="wecC"
                     /locus_tag="RHV13_000382"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HDV1239944.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(31685..32815)
                     /gene="wecB"
                     /locus_tag="RHV13_000383"
     CDS             complement(31685..32815)
                     /gene="wecB"
                     /locus_tag="RHV13_000383"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HDV1239945.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(32871..33917)
                     /gene="wzzE"
                     /locus_tag="RHV13_000384"
     CDS             complement(32871..33917)
                     /gene="wzzE"
                     /locus_tag="RHV13_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HDV1239946.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(33929..35032)
                     /gene="wecA"
                     /locus_tag="RHV13_000385"
     CDS             complement(33929..35032)
                     /gene="wecA"
                     /locus_tag="RHV13_000385"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HDV1239947.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(35272..36531)
                     /gene="rho"
                     /locus_tag="RHV13_000386"
     CDS             complement(35272..36531)
                     /gene="rho"
                     /locus_tag="RHV13_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDV1239948.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(36616..36717)
                     /gene="rhoL"
                     /locus_tag="RHV13_000387"
     CDS             complement(36616..36717)
                     /gene="rhoL"
                     /locus_tag="RHV13_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HDV1239949.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(36858..37187)
                     /gene="trxA"
                     /locus_tag="RHV13_000388"
     CDS             complement(36858..37187)
                     /gene="trxA"
                     /locus_tag="RHV13_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HDV1239950.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            37318..38583
                     /gene="rhlB"
                     /locus_tag="RHV13_000389"
     CDS             37318..38583
                     /gene="rhlB"
                     /locus_tag="RHV13_000389"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HDV1239951.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            38717..40201
                     /gene="gppA"
                     /locus_tag="RHV13_000390"
     CDS             38717..40201
                     /gene="gppA"
                     /locus_tag="RHV13_000390"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HDV1239952.1"
                     /translation="MGSASSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLRQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(40248..42269)
                     /gene="rep"
                     /locus_tag="RHV13_000391"
     CDS             complement(40248..42269)
                     /gene="rep"
                     /locus_tag="RHV13_000391"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HDV1239953.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     ERIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERSESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEKERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            42486..42935
                     /locus_tag="RHV13_000392"
     CDS             42486..42935
                     /locus_tag="RHV13_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDV1239954.1"
                     /translation="MAINFSPKVGEILECNFGNYPVSQNGPFSTTYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNIINDLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            43134..43415
                     /gene="ppiC"
                     /locus_tag="RHV13_000393"
     CDS             43134..43415
                     /gene="ppiC"
                     /locus_tag="RHV13_000393"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HDV1239955.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(43461..44936)
                     /gene="ilvC"
                     /locus_tag="RHV13_000394"
     CDS             complement(43461..44936)
                     /gene="ilvC"
                     /locus_tag="RHV13_000394"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HDV1239956.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            45086..45979
                     /gene="ilvY"
                     /locus_tag="RHV13_000395"
     CDS             45086..45979
                     /gene="ilvY"
                     /locus_tag="RHV13_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HDV1239957.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(46031..47575)
                     /gene="ilvA"
                     /locus_tag="RHV13_000396"
     CDS             complement(46031..47575)
                     /gene="ilvA"
                     /locus_tag="RHV13_000396"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HDV1239958.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(47578..49428)
                     /gene="ilvD"
                     /locus_tag="RHV13_000397"
     CDS             complement(47578..49428)
                     /gene="ilvD"
                     /locus_tag="RHV13_000397"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HDV1239959.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHSDRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRANLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(49493..50422)
                     /gene="ilvE"
                     /locus_tag="RHV13_000398"
     CDS             complement(49493..50422)
                     /gene="ilvE"
                     /locus_tag="RHV13_000398"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HDV1239960.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDALIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(50442..50705)
                     /gene="ilvM"
                     /locus_tag="RHV13_000399"
     CDS             complement(50442..50705)
                     /gene="ilvM"
                     /locus_tag="RHV13_000399"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HDV1239961.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(50702..52348)
                     /locus_tag="RHV13_000400"
     CDS             complement(50702..52348)
                     /locus_tag="RHV13_000400"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HDV1239962.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFEVAISGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGISGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(52351..52401)
                     /locus_tag="RHV13_000401"
     CDS             complement(52351..52401)
                     /locus_tag="RHV13_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1239963.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(52488..52586)
                     /gene="ilvL"
                     /locus_tag="RHV13_000402"
     CDS             complement(52488..52586)
                     /gene="ilvL"
                     /locus_tag="RHV13_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HDV1239964.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
BASE COUNT        12794 a        14350 c        13416 g        12101 t
ORIGIN      
        1 tatgatagcc gaagattttg cctgttcagg caatggttcg atgatttttt tgatttgtta
       61 tttttccact ttcagtatgc taatgcttgc acctactgat tcatcaaaac aatagttatg
      121 gagtatttat gagtaaggaa tatatgaacg atggttcatt aagtgaaaaa tggaaatacc
      181 gatttaattt ttatgatcaa catggctttc ctggattctg gggagcaaca ccagaatata
      241 aagcagcatt taaagcatta aaagtaagac aacgattaac tattcaaatg aatttcattg
      301 ctttcttctg ttcctggatc tatttatttg ttctgggctt atggaaaaaa gcaattatag
      361 ttctattact tggcattcta tctctatttg ttggtgcact aattggcgtt aacattctgg
      421 gaattgccgt agcagcctat gttgctgtta acacgaacaa atggttttat gagaaagagg
      481 taaaagggtt aaatacctgg agcctttgaa taacaggtgg cttagccagg ctaaaccacc
      541 gctctcgttt tttacaacca gttcttccgc ttaaagtaca gatacggtgc caggcccgcg
      601 aggatcataa agataatcgc gcctgggtag ccgaaactcc acttcagttc tggcataaac
      661 tcaaagttca tcccgtagct ggaagcaacc agcgttggcg gcaggaacac gacggatacc
      721 accgagaaaa ttttgatgat gcggttctgc tcgatgttga taaaacccat tgccgcctgc
      781 atcaggaagt tcaccttctg gaacagggat tcgttatgcg gcagcaggga ttcgatatct
      841 cgcaggattt cacgcgcctg ctccagttgc ccacccggta aacgcgcttt acgcaccagg
      901 aagttgagcg cacgctgggt atccatcaga cacaggcgca ctttccagcc gatatcttcc
      961 agttccgcca gcgtggagag cgcctcgtcg tactcatcgc cctgatgccc ttccataatc
     1021 acccggctca actgctccag gtcgctatag atattttcaa tttcatctgc caactgttcg
     1081 attttggttt cgaacagatc cagcagcaac tcgtaggcgt taccgtctac catcgactgg
     1141 ctacgagcac gcatacgata cagacgaaaa gcgggcagtt cacgctcacg cagagtaaac
     1201 agacgaccat cacggatggt aaatgccaca gtggagttac cggcgtgatc ttccgcatct
     1261 tcaaagaaga agaaggagtg aatatgcagg ccgtcatcgt cttcaaagaa acgtgccgat
     1321 gcttcgatgt cttccagttc cgggcgggtt gccaggctct ggccaagttc agattgtacg
     1381 cgcagtcgct cgtcgtcgtc cggttcgaca agatcaatcc atactgcatt tacaaggggt
     1441 tgtgactctt cgacttccag ccgggtcagt cggttatttt ccagttgaaa tgcgctcagc
     1501 atgaccggga ctcccaatgc ttaaaatatc ggacagttcg gtgggcacac agaaacaaat
     1561 tgggtttcag accattaaac agcctgactc agcgcgacgg gaaaaattga ggtcgctgac
     1621 aaccgctaag gctatcagca aaaagggata gccttaggag ttgatcctgg atgacaggat
     1681 agtgagccag tatctactgg gtgtgtccaa ggcgaatgtc ctcttagagt gatcgtgcgc
     1741 gcatgttacg ccagcaaaat tttgccgtca acacgcaaca gaacaccaca gaacaatagt
     1801 tgaacttcgc gctttttggt actcgtcaag gtcgctaaca ggataaaaaa atcccacatc
     1861 caggacctat aatggcgcat cggctactca ttggtaaagg aatgatcacc ttgaatctca
     1921 aacggatttt tctcgccctc accttacttc ccttgtttgc tgttgccgct gatgattgcg
     1981 cactctcaga tccgacgctg accgtacagg cgtataccgt taatcctcaa acagagcggg
     2041 tgaaaatgta ctggcaaaaa gccaatggcg aagcgtgggg aacgttacat gctctgctgg
     2101 cggatatgaa tagtcagggt caggtgcaga tggcgatgaa cggcggcatc tatgatgaaa
     2161 gctatgcgcc gctcggtttg tacatcgaaa acggtcagca gaaggtggcg ttaaatctcg
     2221 cttcaggtga agggaatttc tttatccgtc ctggcggcgt gttttatgtc gcgggagata
     2281 aagtcggcat cgttcgtctg gatgccttca aaaccagtaa agagattcag tttgcggtgc
     2341 agtcagggcc aatgttgatg gaaaacagtg taattaatcc acgtattcat cccaacgtcg
     2401 cctcacgtaa aattcgtaac ggtgttggga ttaataaaca tgggaatgcc gtgtttttgt
     2461 tgagccagca ggcgacaaat ttttatgatt ttgcctgtta tgccaaagcg aaactgaacg
     2521 ttgagcagct gctttatctt gatggcacga tttcacatat gtatatgaaa ggcggggcaa
     2581 tcccgtggca acgctatcct tttgtcacca tgatttccgt cgagcggaaa ggttagaatg
     2641 atagttttgt gtcagatact aaatatggtg ctgatgccgg atgcgacgta aacgccttat
     2701 ccggcctaca tgacgttgca atttattgaa tctgaatgat ttttaggcca aataaggtgt
     2761 acagcaccgc atccggcaac gttataccgt ctccagccgg gcgtaagccg ccaccagcca
     2821 tttgattccc tggccctgga acgccacctg caaacggcta tgttcaccgc tgccttccat
     2881 attgacgatg gtgccttcac caaacttagc gtggcgtacg cgctggccga gcttgtagcc
     2941 gctgtcgttc tcgaccatcg gcgtacccat acgctgatgg ctgaccgggc ggcttaccgt
     3001 ggcgcgcagg cgcacctctt ccacacactc ttccggcaac tcaccgataa agcgcgacgg
     3061 gcgatggtaa acctctttgc catacagacg gcgagtttcc gcgtaggtca gcgtcagttt
     3121 ctgcatcgca cgggttacgc caacgtaggc cagacggcgt tcttcttcca gacgtccgcc
     3181 ttcatccagc gacatctggc ttgggaacat gccctcttcc ataccgacaa taaacacctg
     3241 cggaaactcc agacctttcg ccgagtgtag cgtcatcaac tgcaccgcat cctgccaggt
     3301 atccgcctgc ccctcgcccg cttccagcgc cgcatgggag aggaatgcct gcagcggcat
     3361 taaatcttcg tcttcttcgt tgtagctgaa ctggcgcgtt gccgtcacca gttcctccag
     3421 gttttcgata cgcgtctgac ctttttcgcc cttctcctgc tcgtacatgg tgcgcaggcc
     3481 ggagtcttta attacccggt cagtctgtac atgcagcggc atatcggcag tttcctgcgc
     3541 taaggcgtcg atcagttcca taaaccgctg caaggcactg gctgcacgtc cggcgagggc
     3601 tttttcctgc aacagttcac gacatgcctg ccagagtgtt aactggcgat cgcgcgatgt
     3661 ctgacgtacc acgtccagcg tccggtcacc aataccccgc gttggcgtat tcaccacacg
     3721 ctcaaaggcc gcgtcgtcgt tgcggttggc aatcaggcgc agatacgaga gcgcatcttt
     3781 gatttcctgg cgttcgaaga atcgcatccc gccgtaaata cggtacggca tactggcctg
     3841 caataacgcc tcttccagta cacgcgactg ggcgttgctg cggtagagaa tggcgcactc
     3901 ggcaagcgct ccgccgttgt cctgccaggt tttgatgcgg ttaaccacaa aacgcgcttc
     3961 atcgagttcg ttaaaagcgc aatagaggga aataggctca ccgtccgcgc catcggtcca
     4021 cagtttttta cccagacgcc cgttattgtt ttcaatcagg gcgttagcgg cgctcagaat
     4081 attgctggta gagcggtagt tctgctccag acggatagtt tcagcaccgg ggaaatcatt
     4141 aaggaaacgc tgaatattct ccacctgcgc cccgcgccag ccgtagattg actggtcgtc
     4201 atcaccgacg atcatcactt tgccggtgtc gcccgccagc aggcggatcc acgcgtactg
     4261 aatgttgttg gtatcctgga attcgtccac caggatattg gtaaaacgtt cgcggtagtg
     4321 ttgcaggata tgcggcttgt taagccacaa ctcgtgagcg cgcagcagca gctcggcgaa
     4381 gtccaccaga cccgcacgat cgcacgcttc ctgatacgcc tgatacacct tctgccaggt
     4441 ctgctccacc ggattaccgt agctttgaat atgatgcgga cgcaggcctt catctttctg
     4501 gctgttgatg taccacattg cctggcgcgg cggccactgc ttctcgtcga ggttcatggc
     4561 tttgatcaga cgcttaagca ggcgtagctg gtcttcgcta tcgaggatct ggaaatcctg
     4621 cggcagattg gcgtccatat ggtgcgcacg cagcaaacgg tgcgccagcc cgtggaaggt
     4681 gccgacccac ataccgccct ggctggtgcc catcagttgc ccgatacgat gacgcatctc
     4741 cgccgccgct ttgttggtaa acgtcaccgc cataatcgag tatggcgagc agttttccac
     4801 gctcatcagc caggcgatac gatgcaccag tacgcgcgtc ttaccactgc ccgcgcccgc
     4861 cagcaccaga aggttgctgc gtggcgcggc caccgcttcg cgctgtttgt cattaaggct
     4921 gtcgagcagg taagaaacgt ccattggcac cgccgcgtaa aaataggttg ggcggagaag
     4981 cgcaacgccc tccgccaaaa agctgggtat atatacagat ttgctgatta tatcagcgag
     5041 gtcagagatg ccaaccggga aatttccaga tgcggcagta aacggctgtc ccaggtttgc
     5101 atcagatcgc cattttccgg tctgatccaa caagcctgca ttccgctacg aattgcccca
     5161 cccacgtcag tggtgagatc gtccccgaca tgtaagatct cgccgatcgg cacgttgagt
     5221 ttttccgccg ccaggaagta catatcgctg aacggttttg agcgcccgtg cgggccagcg
     5281 cgcagcacaa actcaaaata atcccccaaa ccaaacagtt ccggctgggc gttaccgttg
     5341 gtgatcgcca ccagcggcca tttcttcgcc agctgtttta aggtgtcgtg ggtttgctgc
     5401 gggacgtcga tccggctgcg ccatttggca aagttgatca ttgctgcgtg tgcgcctgca
     5461 ctggcttctt cggcactcag cccggcgtcg agcatcgctt gttcaatcga acgaaaacgc
     5521 cagcgcgtca catcgtgata aatctcgggt tccgcttccc gtaccgcctg gcgcaggcgt
     5581 tgcagatctt cattctggaa gctgcgcagc gccggatgat aattttgcac aaaggtaagc
     5641 gcctctcgct cggtgcgcaa aatcaccgga cggttatcgt aaagggtatc atccaggtca
     5701 aaggtgagcg ccgagatgcg ccccaaaggc cggtaaaaac gcattatttc ccccgtttgg
     5761 cgcgtggatg cgccgcatcg tacaccgagg caaggtgttg aaaatcaaga tgagtataga
     5821 tttgcgtggt ggagaggttg gcatgaccca gcagctcctg cacaccacga agatcgccgc
     5881 tcgactccag catatgcgta gcgaacgagt gacgtaattt atgcgggtgg acgtgattat
     5941 tcagcccttg ttttatgccc cattcggcaa agcgtttctg cacattacgc gcggagatac
     6001 gcttgcccag tttcgacaga aaaagcgcgt cgtcttcgct accaaacagg tcgcgcaaat
     6061 caagccagtg ctcaatccac gccacagcgt tgcgaccaat cggcaggcgg cgctctttgc
     6121 tgcctttccc catcacccac acttcgccgg actccaggtc gaggtgtttg atatccagcc
     6181 ccaccagctc ggaaagacgc agacccgcgc cgtacatcac ttccagcatt gcacggtcgc
     6241 gtacagcgag gggatcgttg atatcaatat ccagcagccg attcatatcg tcgacgtcga
     6301 tgtttttcgg cagatgacgc ggcgctttcg gtgccgaaac acctttcgcc gggttagctt
     6361 tgagttcgtt ctggctgacc agccagtcaa aaaagctacg tagcgcagaa agccgtaacg
     6421 cgaggcttgc tgctcccagc cctttacggc gactgcgtac agcaaaattg cgtaccatcg
     6481 ctgcatcgca ttgttgccag ctttgcaggc cgttttcgct ggcaaaattg atgatcgcct
     6541 caagctgacg ctggtagtta agtagggtta tcgggctaag ctgacgctcc acgctcagat
     6601 aacgtaggta gcgttctaca tcggtgtgta aatcggtcat acgcgttcaa tccaacgctc
     6661 cagaagctcc ggcaacatca gcgcaatttc atgaagtaac tgcgttccct gcccttgttg
     6721 atagtgactg gcatcgcgac tggtaaacag cacgacaccc aaatcagcat cgcttcccag
     6781 catcgacatc gccaccgatc ccaccgcttt cgcttccggt agcaccacca gcagctctgg
     6841 tccgttaagc ggcccaagat agtgctgttc ctgccccaaa cgctgaatac gcagcggttc
     6901 gaaagactga cggcttaatg ccagatgagt gtggttcgac ggcgcaccta agcgccagcg
     6961 atccggaaac aggcgcagac tcgcacctgc caggccgaga tcgcgcgccc agcggtgaaa
     7021 gcgcatcagc atatcgtcga gactgctggc ggcggtgaga ctgcgctgca ggtagagtag
     7081 gcgataaaac aggccttcgt tggcgatagc ctgttccatc aacagcgcca tgttctcttc
     7141 cagaacatga atatgattac gtgcgcgggc catgtgccac tcgaccaacg aaacggtgcc
     7201 gcgtaccgga tgcggcacac gtatcgcttc tactgcgcgc gcattgcgga taaaaaactc
     7261 aggattttta atcagataat cgacaaccgc ccggtcatca agctccgtga gtgtttcctg
     7321 cagttcttcc cctggttgct tcatagatga ataaatccgt cgtagacatg taccgccggg
     7381 ccagtcatat ataacgggtg acccggacct ttccaggcga tatcaagacg accgccgggg
     7441 agttccacgc gtacttcttc ggccagcaaa ccttgctgaa tccctactgc aaccgccgca
     7501 cacgcgccgc tgccgcaggc ctgggtttct cctgccccac gctcataaac gcgtaaacga
     7561 atatgctcgc gcttaaccac ttgcataaaa ccgatattgg ctcgctccgg aaaacgctcg
     7621 tggctttcca gaacaggacc aagcgtttct accgccgcgg tatcgacatc atcgacctga
     7681 atcacgcaat gcggatttcc catcgacacc acgccgcata agattgtctg ctcggcggcg
     7741 cgcataatat aggtcttttc cgctttgtta gcgcgaaacg gcacagcgga aggttcgaag
     7801 ttgggttcgc ccatatttac gcggaccaga tcatcatcgg tgacggtcag aaccatccgc
     7861 ccgttggcgg tgctgacgcg gatatcgcgc ttattggtca gccctttcag gcgcacaaaa
     7921 cgggcaaagc agcgcgcacc gttgccgcac tgcgccactt cactgccatc agcattgaaa
     7981 atgcgatagt gaaaatccag ttcaggatca tacggcggct caaccaccag cagctggtca
     8041 aaccctaccc ccaggtgccg atccgccagg cgacgaatca gctccggtga aaaaaagaca
     8101 ttctgcgtta ccgcgtcgac gaccataaaa tcgttgccaa ggccatgcat tttcgagaac
     8161 tgcatcattt actccaatca cgcgggtaca gaaactgact tttagtaatt cacctgggat
     8221 ggaccatcgc cagtggcgcg atcgttttta tccggcaccg tggattgcgt ttgcgtctct
     8281 accggtttgg tcggcggcgg tgcattttta tctgcaggcg ggaaatagag cggacctttc
     8341 agaccgcagc ccgtcaggct gaagagagta agtaatacag tgagtgcctt aaacacgttt
     8401 ttcattatgg attgcctgta aagttcatcg ctttctgctt tctatcatcg caggagaagg
     8461 cgtaaaagca agcatttagc gactctttat gaagtctgaa aagtgaggaa atgctgcgag
     8521 tataacgctt actcgtgggg atttatgttc tgctggcagt ttcgtgatca acacctcgcg
     8581 ccaggatggc tttatgcact atcgcaatgt ggtttgtttg ttggcttttt tagttccgtc
     8641 agcgtgagga tgctcgctcg gtaaacctga tcataatgtt taccagaaac agggaaaggc
     8701 gtggtgtaac tgcgccctaa tgaagagaca aatctttcgc tacactgaga catcattatt
     8761 ccggtgacgg atgaatcgtg ccggaacgca gccgcagagg tgcaaacttt gaagccctga
     8821 ccccatgccg ctatactgcc gccatcactt aaaaacagga tacaacaatg aacgacagtg
     8881 aatttcatcg cctggctgat caactctggc tgaccattga agaacgcctg gacgactggg
     8941 atggcgacag cgatatcgac tgcgaaatca acggcggtgt actgaccatt acctttgaaa
     9001 acggcagcaa aatcattatc aaccgccagg aaccgctgca ccaggtatgg ctggcaacca
     9061 aacaaggcgg ctaccatttt gatctgaaag gcgatgagtg gatttgcgat cgcagcggcg
     9121 aaaccttctg ggatttgctg gaaaaggcgg cgacgcagca ggcgggtgaa acagtcagtt
     9181 tccgctaaga ttgcatgccg gataagcctc gctttccggc acgttcatca cgaaaaatac
     9241 tgctgtaata gcggcgtatc gtgatcctga ttggcaggcg gcatgttgcc gatagatttg
     9301 gtgcggaacg gaatcacctg ttcacgacca tcaaccttca caatctgata gaactgcggc
     9361 aggttgaagt tgatgaagct tgagccgtag gtaaaacggt catgcgatga cgagtagaag
     9421 cgactgacgt cacgcaccag ctcctctttg ctgccttcgc agtggtgata cacctcaacc
     9481 cggttgcttt cgtcgagaat gtagatatta aagccattct cgtcttgcgt ttcttcgaaa
     9541 aagaactgga tgatcccttc gctggcaaag ccgtccacca ccgccggtaa tttgacgtga
     9601 ttggtttcaa cctgcactga caggccgtgc agtttgttat gcgaaatagc gccataaaac
     9661 tcgatggcgt tttccagttt ctgtaccgat acattcaggc gttcgaagaa caacccccag
     9721 gtttgaccag aaacgcgcag cgccttgaaa cgcccggttt cctggcgggt gctggaaagg
     9781 cgcaattcaa tacactcaga aaccagttgc tgcacgcgag tacggattaa cccgcgcaga
     9841 tgctggctat aacagaagac ttccacgcta tctggcggtg cggcgtcctg atgcattttg
     9901 ccgagaatag ttttcagggc ttcgatcatc gattgctcgc cgttgaagtg cagcgtacgc
     9961 acttcgttcc atgagttgcg gtacagcagg tcaacgctac ctaccaggca attttgattc
    10021 tcgccaaagc tgaagacatc cagcttacgg aaatcgaaat gcaccacctg attgcggaac
    10081 gccgctgtcg ggtcatattc caggttaacg ataatcgcca gatggcggat ctcacacggg
    10141 ctgtagagcg ccttcggtgt cggtgcaggt aagcgcagcg ggaaatggtg cgacacgtcg
    10201 gcgaccatct cctgcaactt aggcaaatcg acaatgccgt tacctttaat atacaaacgg
    10261 gtgcgcgagg tcagcaggcc attaaaccat gcccacgcca ccagtttatt caggtaacgg
    10321 ttatattcca gcggctgatg gctgatgatc gactcaatat ttggcgcgcg gttatacaga
    10381 taccaacctg aacggttagc acggcccggc ggcacataaa taaaggtcag attcggttcc
    10441 gagagatcgg gtgaaatctg cgggtttacc agcgttactt tacctggcaa tgcttcaaac
    10501 gcggcataca gtttacgcgt cagtacgccg atatcctgcg gactggcgga gacgctaagg
    10561 ttattgcgac gcgcaaagcg gatcagatta cggtagctct gcatcatcgc gtcgagcagc
    10621 tcgttgtgcg cctcacgcac ctgatcaatc ttccagttag cacggttatc gagcattgcc
    10681 agacgagctt cgtcccaacc ccactcgctc actaactggc tcaacactgc gcgacgccag
    10741 cctacacagg cgcgttcacg gctgagcttt tcgcacactt ttaaatagaa gcagcgacgt
    10801 actaaatcca gacgagtgaa atcttcaatc gccgtcaggt attcagtaac acgctccagc
    10861 atcatgcagt acggatcgag gccaaacgat acaatctcgc cgtcgtgcaa acgctgtttg
    10921 atatctttcg ccagcagacg tgggttcggg tattcccagg aataggcttc cagcagcagt
    10981 gttttcagta ccgctttgta tggggaatcg atactcttgt agagctgcca aaggctggca
    11041 ccaaagtact cttcagcaga aagcgagctt aagccaccga gatccagcca ttcatttggc
    11101 gtcagcacgc cctgcgcgta aagcgtcatc acatagtcgt cgtaatgctc ttcttcgtcg
    11161 cacggcacca tattccacag aatacgctta ccggcgagac gcacggcggt acgataaaat
    11221 tcgtcaagca gcagtatatg ctgggtggag ccacaatctt cgccccccag gctgccgctt
    11281 tcattatgac ggaagcggtt ttcatcaatc aggaagaagc tgacttccac acccagcgag
    11341 gcggcccagc tttccagcag gctacattta cgttgtagca actggcgctc ttcgctatcg
    11401 agccaggatt gatgacagac ccagatatcc aggtcagagg aacaactttg ccctacggac
    11461 gaagtgctgc ccatggtgta tacaccagta attggaagct cacctttcgg cgggtcctgt
    11521 actgacattc cacgatacag ttcaagctcg ttcaggtagt ggcgttgagt ttcatcaggc
    11581 gtgtaaaggc aaatgccttt gggaacgtta ccatcaaggt aacccggcat tagcggatgg
    11641 tgatagtgca acaatgtcgg cagtagactg tagacctgtt ggaatgcagg ccccatagca
    11701 gcaagcgcgc gatccacacg caattgattt atggcatcca gtctctgttt cagagtctca
    11761 atatagaggt acaagacgta tcgcctgatt tgctacccgt catgactgtg attccgccaa
    11821 catcaacggt aacacgcggc attcgggata tttcgtatgt caaaggtaac cgttaccact
    11881 tttcgcgcct ggttttttta gtttcacgac gaaaaaatgg tctaaaacgt gatcaattta
    11941 acaccttgct gattgaccgt aaagaaagat gcgctacata caagtgtagc accgtttatt
    12001 ctctgtaaat tccttattac gacggcgtga aacgcctgtc aggatccact gccagacctc
    12061 attttacggt ttgcgcaggc gcctacgttt caccacaaca ctgacatcac tctggcaagg
    12121 atgttaggat ggaccacgga tgataatgac ggtaacaagc atgttagaca atgttttaag
    12181 aattgccaca cgccaaagcc cacttgcact ctggcaggca cactatgtca aagacaagtt
    12241 gatggcgagc catccgggcc tggtcgttga actggtaccg atggtgacgc gcggcgatgt
    12301 gattcttgat acgccgctgg cgaaagtagg cggaaaaggc ttatttgtaa aagagctgga
    12361 agtcgcgctc ctcgaaaatc gcgccgatat cgccgtacac tcaatgaaag atgtgccggt
    12421 tgaattcccg caaggtctgg gactggtcac tatttgtgag cgtgaagatc ctcgcgatgc
    12481 ctttgtgtcc aataactatg acagtctgga tgcgttaccg gcaggcagta tcgtcgggac
    12541 gtccagttta cgtcgccagt gccaactggc tgaacgccgc ccggatctga ttatccgctc
    12601 cctgcgcggc aacgtcggca ctcgcctgag caaactggat aacggcgaat acgatgccat
    12661 cattcttgcc gtagccggac taaaacgttt aggtctggag tcacgtattc gcgccgcgtt
    12721 gccacccgag atttctcttc cggcggtagg gcaaggtgcg gtgggtattg aatgccgcct
    12781 tgatgatgca cgcactcgcg agctgcttgc cgcgctgaat caccacgaaa ctgcactgcg
    12841 cgttaccgca gaacgcgcca tgaatacccg tctcgaaggc ggatgtcagg tgccaattgg
    12901 tagctacgcc gagcttattg atggcgaaat ctggctgcgt gcgctggtcg gcgcgccgga
    12961 cggttcgcag attattcgcg gtgaacgccg cggtgcgccg caagatgccg aacaaatggg
    13021 gatttcgctg gcagaagagc tactgaataa cggcgcgcgc gagatcctcg ctgaagtcta
    13081 taacggagac gccccggcat gagtatcctt gtcacccgcc cgtctcccgc tggagaagag
    13141 ttagtgagcc gtctgcgcac actggggcag gtggcctggc attttccgct gattgagttt
    13201 tctccgggtc aacaattacc gcaacttgct gatcaactgg cagcgctggg ggagagcgat
    13261 ctgttgtttg ccctctcgca acacgcggtt gcttttgccc aatcacagct gcatcagcaa
    13321 gatcgtaaat ggccccgact acctacttat ttcgccattg gacgcaccac cgcactggca
    13381 ctacatacca taagcggaca gaagattctc tacccgcagg atcgggaaat cagcgaagtc
    13441 ttgctacaat tacctgaatt acaaaatatt gcgggcaaac gtgcgctgat attacgtggc
    13501 aatggcggtc gtgagctaat tggggatacc ctgacggcgc gcggtgctga ggtcactttt
    13561 tgtgaatgtt atcaacgatg cgcaatccat tacgatggtg cagaagaagc gatgcgctgg
    13621 caatcccgcg aggtgacgac ggtcgttgtt accagcggtg aaatgttgca gcaactctgg
    13681 tcgctgatcc cacaatggta tcgtgagcac tggttactac actgtcgact attggtcgtc
    13741 agtgagcgtt tggcgaaact cgcccgggaa ctgggctggc aagacattaa ggtcgccgat
    13801 aacgctgaca acgatgcgct tttacgggca ttacaataac tctcataaca ggaagccata
    13861 atgacggaac aagaaaaaac ctccgccgtg gttgaagaga ccagggaggc cgtggacacc
    13921 acgtcacaac ctgtcgcaac agaaaaaaag agtaagaaca ataccgcatt gattctcagc
    13981 gcggtggcta tcgctattgc tctggcggcg ggcgtcggtt tgtatggctg gggtaaacaa
    14041 caggccgtca atcagaccgc caccagcgat gccctggcta accaactgac ggcattgcaa
    14101 aaagcccagg agagccaaaa agccgagctg gaaggcatta ttaagcaaca agctgcacaa
    14161 cttaagcagg cgaatcgtca gcaagaaacg ctggcaaaac agttggatga agtccaacaa
    14221 aaggtcgcca ccatttccgg cagcgatgct aaaacctggc tgctggctca ggccgatttt
    14281 ctggtgaaac tcgccggacg gaagctgtgg agcgatcagg acgtcacgac cgctgcagcg
    14341 ttgctgaaaa gtgcagacgc cagcctggcg gatatgaatg acccgagtct gattaccgtt
    14401 cgtcgggcaa ttaccgatga tatcgccagc ctttctgcag tatcgcaggt ggattatgac
    14461 ggcatcatcc ttaagcttaa tcagctttca aatcaggtag ataacctgcg tctggccgat
    14521 aatgacagcg atggttcgcc gatggattcc gacggtgaag aactttccag ttccatcagc
    14581 gaatggcgta tcaatctgca aaaaagctgg cagaacttta tggacaactt cattacgatt
    14641 cgccgtcgtg atgacaccgc cgtaccgctg ttagcgccaa atcaggatat ctatctgcgc
    14701 gaaaatattc gctctcgcct gctggtcgca gcacaagctg taccgcgtca ccagcaagag
    14761 acttatcgcc aggcgctgga gaacgtctcc acctgggtac gtgcttacta cgatactgat
    14821 gatgccacca ccaaagcgtt cctcgacgag gtggaccagt taagccagca aaatatctcg
    14881 atggatcttc cggaaaccct gcaaagccag gcgatgctgg aaaaactgat gcagactcgc
    14941 gtgcgtaacc tgctggcaca accggcagcg gggacaacgg aagctaaacc tgcacctgca
    15001 ccgcaagctg caccgcaagc tgatactccg gcagccgcgc cgcaaggaga ataaccatgc
    15061 taaaagtgtt attgctcttt gtgttgctga ttgcggggat cgtggttggc ccgatgattg
    15121 ccggtcatca gggttatgtg ctgatccaga ccgacaacta caatatcgaa accagcgtca
    15181 cgggcctggc gatcatattg attctggcga tggtagtgct gtttgccatt gagtggctac
    15241 tgcggcggat cttccgcact ggcgcgcaca cccgtgggtg gtttgtcggg cgtaagcgtc
    15301 gccgtgcacg taagcagacc gaacaggcgc tgctgaaact ggcggaaggc gattatcagc
    15361 aagttgaaaa gctgatggcg aaaaatgccg atcacgctga acaaccggtg gtgaactatc
    15421 tactggctgc cgaagccgcg caacaacgtg gtgatgaagc gcgcgccaac caacatctgg
    15481 aacgcgcagc ggagctggcc ggcaatgaca ccattccggt agaaatcacc cgtgtacgtc
    15541 tgcaactggc ccgtaatgaa aaccatgctg cacgccacgg cgtggataag ctgctggaag
    15601 ttacgccacg ccatccggaa gtattacgtc tggcggaaca ggcgtatatc cgcacaggtg
    15661 catggagttc gctgctggat attatcccat caatggcgaa agcccatgtt ggtgatgaag
    15721 aacatcgtgc aatgctggaa caacaggcat ggattggcct gatggatcag gcgcgtgccg
    15781 ataacggtag cgaaggtttg cgtaactggt ggaaaaacca aagccggaaa acgcgtcatc
    15841 aggtagcgtt gcaggtggca atggcggaac atcttattga atgtgacgat catgatactg
    15901 cccagcaaat tatcatcgat ggcctgaaac gccagtacga cgatcgccta ctgctgccga
    15961 ttcctcgact gaaaacaaac aatccggaac agctggaaaa agtgctgcgc cagcaaatca
    16021 aaaacgtcgg cgatcgtccg ctgttgtgga gcacactggg ccagtcactg atgaagcacg
    16081 gagaatggca ggaagcatcg ctcgccttcc gcgcagcgct gaaacaacgt ccggacgcct
    16141 acgattacgc atggcttgcc gacgcgctgg acagactgca caagccggaa gaagctgcag
    16201 cgatgcgtcg cgacggtttg atgttaacgt tgcagaacaa cccaccacag tagttccttc
    16261 tcacccggag gcaagcacct ccggggcctt cctgacacat aaaaaaacgc ctgctcttat
    16321 tacggagcag gcgttaaaac aggtctgtat gacaacaagt gggtgcttca ctcaacgttg
    16381 tgtccatggt gtctgatgag gcataagcga catctgtcag tggacgataa gcaccgtaaa
    16441 cggctctgcg tcattccgga gtttatgagg cactaaggcg aacataagag atggaatgag
    16501 catctactcg tttattatgc cacagagaat cgggaaataa catcccttaa cacttgttat
    16561 gagataattc tgtaatcctc ttggcttcct gagtaataac tttctgagtg aatatttacc
    16621 ctgagcttga ttctacacat acttattatg aatgataaaa ttcattcaat taataacaca
    16681 tatattaatt gccgttaaaa ctaaaaacag catcaataat caacgcgata taataaacct
    16741 gccttacata tcaactgcgc cagaggtagg attgaaaacg ctctcctgat ttttaaattc
    16801 atttcttgga taaataaata atttattttt atcactatta tttatgtaat catcctgtca
    16861 gggagaggga tctcaattat caatgcttaa ttacgtcatc attttgttga catgaaaccg
    16921 gagcattttg catggaattt tcgttttcac ccaaacttct tgttgttgct gtcgccgccg
    16981 ctcttcctct catggccagc gcagcagata ccccgtcaac tgccaccgca cgcaaaggct
    17041 ttgccggata cgatcaccca aaccagtatc tggttaaacc ggcgaccact attgccgaca
    17101 atatgatgcc agtaatgcag catccggcgc aggataaaga aacccagcag aagctggcag
    17161 aacttgagaa aaaaaccggt aagaaaccga atgtggttgt tttcttgctg gacgatgtgg
    17221 gctggatgga cgtcggtttt aacggtggcg gcgtggcggt gggtaaccct acaccagata
    17281 tcgacgccgt tgccagccag gggctgattt taacttcggc gtattctcaa ccaagctctt
    17341 ccccaacccg cgccaccatt ctcaccggac aatactccat ccaccacggc attctgatgc
    17401 cgccaatgta cgggcaaccg ggcgggctgc aagggttaac cacgctgccg cagttgctgc
    17461 acgatcaggg ctacgtcact caggccatcg ggaaatggca tatgggggaa aacaaagagt
    17521 cgcagccgca gaacgttggc tttgatgatt tccgtggctt taactcggtg tctgatatgt
    17581 acaccgaatg gcgcgacgtt cacgtaaacc cggaagtggc cctgagtccg gaccgttctg
    17641 aatacatcaa gcaattaccg ttcagcaaag atgacgttca tgcggtgcgc ggcggcgaac
    17701 aacaggccat tgccgacatt acgccgaaat atatggaaga tctggatcaa cgctggatgg
    17761 aatatggcgt taagatcctc gacaagatgg cgaagagtga taagcctttc ttcctctatt
    17821 acggcactcg cggctgtcac ttcgataact acccgaacgc caaatatgcg ggtagctctc
    17881 cggcacgcac ctcgtatggc gactgcatgg tggaaatgaa cgatgtgttc gctaatctgt
    17941 ataaagcact ggagaaaaac ggtcagcttg ataacacgct gattgtcttt acctccgata
    18001 acggaccgga agccgaagta ccgccgcacg gacgcacacc gttccgtggt gcgaaaggat
    18061 ctacctggga aggcggcgtt cgcgttccga ctttcgtgta ctggaaaggg atgatccaac
    18121 cgcgtaaatc tgacggtatt gtcgatctgg cagatctctt ccctaccgcg ctggatctgg
    18181 cagggcatcc tggcgcgaaa gtggcgaatt tagtgccgaa aaccaccttt atcgatggtg
    18241 tggaccagac atctttcttc ctgggaacaa atggtcagtc taaccgtaag gccgagcact
    18301 acttcctcaa cggtaaactc gctgctgtgc gtatggatga attcaagtat cacgtcctga
    18361 ttcagcaacc ttacgcttat acccagagcg gatatcaggg tggattcacc ggcacagtaa
    18421 tgcaaacggc gggatcgtcg gtgtttaacc tgtacaccga tccgcaggaa agcgactcca
    18481 ttggcgtgcg ccatattccg atgggtgtac cgctacagac cgaaatgcac gcgtatatgg
    18541 agatcctgaa aaagtatcca ccacgcgcgc agattaaatc tgattaagcc ggcgctatcg
    18601 ctgagatctg cctttgccgg atgcgatgct gacgcatctt atccagccta cagaacgctg
    18661 caatttattg aatttgcacg atcatgtagg ccggataagg cgtttacgcc gcatccggca
    18721 atcaaccgca ggcggccaca aaatggccgc ttttttactt actcaccacc agcaaatgcg
    18781 cctgcataat gtcgctggcc gggcgaccgt gcgccagcaa atcagccata gctttaagat
    18841 atggcggtag atggcggaaa taacgctgat atccggcaca caaataattc agccccggtt
    18901 taccgctagc atcgagcatg aagcggtgtt tcgggcagcc tccccaacat gcttttaaca
    18961 cgttacaact gcgacactgc gccggtaact gcttaaattt atcttcacca aacacctgct
    19021 gttgcgggga atcgatcatt tctgcaattg tttgctggtg catattcccc aaccgatatt
    19081 gcggataaac atagtgatcg caggcgtaaa cgtcgccgtt gtgctcaaca atcaccgagc
    19141 gcccacaggt tggctgatga tggcaaaccg cacccggcgc accgacaaaa ttggcaaacg
    19201 cccattcgat attcatcacg aaaatcttgc cgacgtcgcg tttaatccag tggtcgaata
    19261 tcgccaccag aaattcacca aattcatcgg ggcgcaccga ccattccgtc agttccccct
    19321 gaatatcacc aggcgcatgt agtttcagtc catcgcgggc agcggtttca tcagtcaggc
    19381 gctcgaccac cggaataaac tggatgaatt ccactcccgc atcgcacaaa aaatcatata
    19441 cctgcaacgg ttgctgcgcg ctggtgcgat taacgcagac cagcacgtta tagtcgacat
    19501 gatgtttttg cagcagcgtc agggcacgca tcaccagttt atgcgtgggt ctgccgcctt
    19561 tggtcacgcg atattgattg tggatctcag ccggaccatc cagcgataac ccaacaagaa
    19621 aatgattttc tgccagaaat gcacaccatt catcatcgag cagtacgccg ttagtctgga
    19681 agctgttact tatcttcctg ccagcaccat atttcgcctg tagagccacg gcacggcggt
    19741 aaaaatccag cccgagtaac gttggttcgc cgccctgcca ggtaaaagcc acttcgtttt
    19801 gggtttcgct ggcagcgata tagtgacgga catacgcttc cagcgtgtca tcgtccatat
    19861 gcgtgactgg cttttcgcgg taaagggttt gtttttcgag ataaaaacag tagtcacagt
    19921 tcagattaca atcggaaccg ctcggtttcg ccatcacatg aaaagcacgc gttggaacct
    19981 gttgcagcat ggtcgctcct tgcctgaagt catccttcaa tacgttaagg gcaaaatcat
    20041 accgtggggc aatgagttat tacgggcgtt tattgcgaag ggagagtgac gaaatgcaca
    20101 gaaaacaaaa aaccccgccg aagcggggtt caaaattggt cggcgagaga ggattcgaac
    20161 ctccgaccca ctggtcccaa accagttgcg ctaccaagct gcgctactcg ccgaaatact
    20221 gctttttgaa tttttagttc aattctttaa agtcgtggtg cgaggggggg gacttgaacc
    20281 cccacgtccg taaggacact aacacctgaa gctagcgcgt ctaccaattc cgccaccttc
    20341 gcattgtcac aacttctaat aatggggtgg ctaatgggat tcgaacccac gacaactgga
    20401 atcacaatcc agggctctac caactgagct atagccacca ctacaaatct tgttacgcgg
    20461 tattactacc accgcagctc aagcgccggg actaaatggc gcgcccgaca ggattcgaac
    20521 ctgagacctc tgcctccgga gggcagcgct ctatccagct gagctacggg cgcttagcgc
    20581 cgttgcgggg gtggataata cggacttccc acccctctgt ctagtccctt tttaaataaa
    20641 ttgcgcgttt ggttacggtt tgtgcgtttt gctgcttatt cctccagttt atgcgctttc
    20701 ccgtggcgat taaggccaaa aactttataa atcgccgtca ccgccagcat aaagatgatg
    20761 ccaacaaaca gcgacatacg cgtatcttca ttaaagtaca tgccgatcaa aacgcaaatc
    20821 aggaatgcca ttgttacgta gttggcccac gggaacagga ttgagcggaa cggatggctg
    20881 gcaatcgccg ctttatgtgc acgccgaaaa cgcagctggc ttatcaatat cacaaaccat
    20941 ggcaccatcc ccggaagcac gctggcactg tagacgtaga caaacacacg ctgcggattg
    21001 ggaatgatgt agttcaggca tgagccaatt agcagaatag caatagatac tgccacaccc
    21061 gcaacgggta cgccgtgacg ggaaactttc gccatcgccg ccggtaactg acggtttttc
    21121 gccagtgcgt agagcatacg tccgcaactg tacatgccgc tgttacagcc agagagcgca
    21181 gccgtcagca ccacaaagtt gataatgccc gccgctgcgg taataccgat tttggcaaaa
    21241 gtcagtacga acgggctgcc gttgctgcct atttcattcc acgggaagat ggtgacgata
    21301 acgaaaatcg cgcctacgta gaaaatcagg atccgccaca gcaccttgcc tacggcactg
    21361 cgcagcgtca cctgcggatt cttcgcttca ccggcagtaa tgccaatcag ctccacgccc
    21421 tggtaggacg ccaccacaat acacagcgcg gtcaggaacc ctttccaacc acccgcaaag
    21481 aaaccgccat gctctgtgag attgctaaaa ccaatcgact gcccgccatt gccaaagcca
    21541 aagaaaatca cgcccaagcc aatgacaatc atcacgataa tcgtggtgac tttgatcatc
    21601 gcgaaccaga actcgatttc gccgtacaac cgcaccgccg ccagattcgc caacgccacc
    21661 agcgccaccg cgatcaatgc gggtatccac tgcgccatct ccgggaacca gaactggaca
    21721 taaacgccaa tggcggtgat ttcagagatc cccacggcca tccacataaa ccagtaagac
    21781 caggcggtga gatagccaaa gaacgggctc atataacgat gcgcataaac ggcgaacgaa
    21841 ccggtaaccg gttcgaggaa caacatttcg cccattgaac gcatgatgaa aaagacgaac
    21901 agcccggcga tgatataggc caacaatacg gatggcccgg cccatttcag ggtactggcg
    21961 gcccccataa acaggccgac gccaatggtg ccccccaggg cgatgagttc gatatgtcga
    22021 gcttccagcc cacgctgtag ctctggtttg ttatctgcca taaatcctcg tgttgtgttt
    22081 gcatgctttc cggtgttacc ggttatcgtt atgggtacat cgagtgttgc gaatgttttc
    22141 gtaattcagg agaaatggca aataaagcat taaaaatttg aatgctttgt gtaataaaaa
    22201 agcagacagg cgacggagtg accactccgt cgctttacaa agagaggaaa atcagaggtt
    22261 gccggtgtaa tgccagcgta aataacgcag caaacgaagc tgacgcttaa tgcggctcgg
    22321 ctgcgaaagc aggcggtaga gccactccag tcccagcgtt tgccagattt tcggtgcgcg
    22381 ttttacgtga ccggtgaaaa catcgtaagt cccgccaacg cccatataca gcgcatctgg
    22441 atgcaccaga cggcagtcgc gcatgaagat ctcctgcttt ggcgatccca tcgcaacggt
    22501 gacgatttgc gctccgctgg catgaatgcg ttcaaacagc gcctgacgct gctcgggttt
    22561 aaaataacca tcctgactgc caacgatatt cacattccat tggttgcgca gtttagcttc
    22621 agtttgcgcc agcacttcag gtttaccgcc cacaagaaat accggcgtcc cttctttgcc
    22681 tgcgcgcgcc atcagctctt cccagagatc ggcaccggca acgcgggaaa cctgcgcctg
    22741 cgggtacttt ttacgtactg aacgtacaac gctgatgcca tccgcatatt taaattcggc
    22801 agcgttaatt aactccctga cctcggcgtt atcttcaatg gtcagcattt tttcagcatt
    22861 aatggcaacc agcgttccct gcttaagctg cccgtcagca aacagataat cgagggcgtg
    22921 ctgcatatca cgccaaccaa tcaactgtaa gccacgcagc gtataggtcg gtgccgtggt
    22981 gttgttattc attgttatcc ttcaacctgc gtccggagcg atgattttgt acgtttatga
    23041 atgagtccgg cgctttcaaa aagccagtac aacagttttg cgatcatcag acatgcgccg
    23101 aagaccacga taaaaaagac tacgcgtgag acaaacgaat ccagcccttc acgtgccagc
    23161 acgatcatat tgaagatcgc cccaaagcag aaactgtgca atatcgcagc cttatagcga
    23221 ttagtctcgc ggttgcccag ctcatacagc cagtcgaacc atttgatgat cagtccaacc
    23281 acaatcgccc cgagcgggat gaacaacgcg ccgcccatca ccaccagtga gcctataagc
    23341 gtaggcgaga tcgccagtcc ggagtggtta ttcagcactt cccaggtaaa gtagttggct
    23401 gagttcagca ccatactcgg gcgacccggc cacagccagg aagggataaa gacatagaaa
    23461 tcgcggacaa ttggcgccag gccctggaag tcgatgttgt cgtagttctg caacagcaac
    23521 gccagattct cccacggcga gaaggtgtcg cgagtgagat agagaaacgt atagaacgct
    23581 tcatcgccgc tcacattcat tccatagcgt tttagcgcca gccagaacat gccaacaatc
    23641 cccagcacgc ccgccgccgc cagcatccac aacgaaatcc agccgcgaat aatgccaata
    23701 aacaggaaga tagcgaatgc gatgatgata ttggcgcgag tgccgccgac aatcatataa
    23761 gtcagcaagc caaaggcgac cgtgctgacg aggaaaaaca gccacgcttt gctgtcctgg
    23821 cgcagaaagt agaccaccag catcgccggg atgaaaaagt aaaagaagcg ttttaacgcc
    23881 acgccggaga cttcactgga aaagatctga ctgtaggagt taagccggaa cagcaaaaag
    23941 ccgttgtgca tgaagaagat gccgacgctt accagcgcga tacccatcag gatcacccac
    24001 gtaagattgg tctccacgcg gttcatggta aacagcggac ggcgcggtac atcagcaacg
    24061 cgtttgcgta ggcgggtttt gtaggtgaca tagtaaaccg cgtagaagca gcccgcagaa
    24121 agcaacgcct gcaacaagat ttctggcggc gcgacaccaa catcaaagcg aaataccagc
    24181 acgctggtca gcgggaagcc gaagaaaaag gtgagcaaaa acagcaatga aaagaagaca
    24241 ttgaagttaa agcgcacacg gcgaaactca aaccaggtca gcgtggcaat aaacagcgtg
    24301 cagagcagcc agaccacaaa caggccactg aattgcagca gactcatgcg acctccccgg
    24361 cggcaatcgc caacgcccgc tgccagcctt gtagatagtt agggctaaag aaggcaatgg
    24421 tgtttttatc caccgacgcc aactggcgct gcgcttcacg cacaatatcc tcgttgagat
    24481 cgtcggtagt aaacagcacc ggcaaatgct gttccgtcat atcctgccag aacggatttt
    24541 cccggttaag cacacaagga atgcccgcct gaatcagtaa gcacagcgta ccaatgccct
    24601 gctggcgggc aaaaataaag taaccgagat cgcactgacg aagtagcgcc agataggcgt
    24661 caaattccag tttttcgctc agaacttgca gattttcttc gctgaataac tccagccccg
    24721 cctgacgaac ttcctcaatg tacgcttcgt tattaggcgg atatcccatc ggcaccacca
    24781 cttttaccgt atcgccaaat tgctgatgaa cggcacgtaa ggcggcgaca tgctcattgc
    24841 tgcggtcgcc ggagttcccc accagaatgg tcattttccc ttcacgttgc cgatcgttcg
    24901 ccatcgtatt gagagaaggg tccatccgcg tcgggaagta cagcagttcg ccccgcacct
    24961 ttggatgcgt tttggcaaaa aagctcaaat caccgcgggt ggcaaataca cagccgactc
    25021 gcttttgcgc caggcgacgt agcgagtaaa aaagcttata tctcaagcca ctggaaagct
    25081 catacagatc tgccccccag atatgccaga aaaactggcg ggacttaatg ccaccactca
    25141 gcaaagccag ccacagtttg ggattgaact gaccgtggaa gaaaaaacgc tgctgacggt
    25201 tagcttttgc tttcgcgatg accgcttccg ccagcgattt tttcccaggg aaaaattgca
    25261 cagaaagcgc cggacagtta tcacttaagc cgtcgtcctt gccaacaacc ataaactcgc
    25321 gcgcgtgctc gctcgtcgcg gccagcgcgt cattgaaaaa ccgcaaaacg gttcggttat
    25381 ggtgagggat atccgatccc agtacgtgaa tcagtacagt catgcccgcc tacgccagag
    25441 taaaaacacg ccacaacaaa gagaaaaata gacgatatag gttgccatat aggcctgcgc
    25501 cgcgcccagt gcaccatgcg cagggattag ccagtgggca aataccatca ataaagtgaa
    25561 ctggctgact tccgccagaa tataaaaccg cagtgaagct ttggcgatca ccagataacc
    25621 aaagacataa gcgcccactt ttaacacatc acccactaac tgccaggcaa agagatcgcg
    25681 catagcggta aatttattcg acaacagcag ccagatagca aaatcacgca gcaaccagac
    25741 ggtaaaactc gccgccgcca ctgccggtaa gacgaatttc agcgatttaa ccacttcccg
    25801 ggtgatatcg cgcttttccg ttagccgcga caacgtgggc agcaaatata cgctgaacga
    25861 tgccgtaata aattgcaggt aggcatcgga aatactgctc accccttgcc agatccccac
    25921 ctcatcccag ctatactgcg ccgccagcag tttacgcatc atgatgtaag caacaggcaa
    25981 ggtcaccgac gtaatcaacg ccatgagcgt aaatttgctc aactgccctg ccagaccgtt
    26041 atcccagctg ggtttcagat agcttaacgg gatgacacca cgtttgatca acatgatggc
    26101 ggcaggaatt accaccagcg cgggaatcag cgccagaccc agcaacgccc cttcataacc
    26161 gcccaaacgg taactgacgt agtacgcaag aacgccaatc aagctgccaa caatcagcga
    26221 taacgcatta cctgcggcat cgcgaaagcc tttcatcaac gccagtaaca ggttgcccca
    26281 ggcgatcccc atttgcacca acgccactaa acgtaccagc ccctgatagt cggtattacc
    26341 aaacaatccc tggctgattg gcgctgctgc cagcacaaaa accagcgcca tcagcgtaga
    26401 gaaaccaagt accatcgctg atgaagtgcc gaccacgcgg cgcagctgtt gcggattatc
    26461 atggtactgg gcaacgtatt tggttacacc gttaaagatg ccagctccgg caagcacgcc
    26521 gagcacggta atcaactggc ggaaatttgc cgccagccca agccccgccg gaccaaatga
    26581 caccgccagc aacttaccga ccagtaaccc ggcaccaatc ttgaccagtg tactggccgc
    26641 cgtccacaag gacgcttttg ccaacgacat atcaggagaa gtagttcaac aaagtcgcaa
    26701 ttaccgtacg ctgattgacg ggcgacaggt tgtagaacag cggcaggcgc agcaggcgct
    26761 cgctctcttt ggtggtgtag ctatcttcac cgtggaactc accaaagcgt tcccccgcag
    26821 ggcaaccgtg cagcggaatg taatggaaca ccgccatgat ttccgcttct ttcagaaagt
    26881 taatcaacgc gctccggtca tcaatatccc gcagtttaat gtagaacata tgcgcgttct
    26941 gcacgcagcc atcgggaatc gacggcagct cgatacgccc ggctttcgcc agaggcgcta
    27001 acgcatcgta gtagttttgc cacagcgcca gacgttgctg gttgatacga tccgctgctt
    27061 ccagttgcgc ccacaggtac gcagcttgca gatcggacat caaatagctg gagccaatat
    27121 cgcgccaggt atatttatcg acctgaccac ggaagaactg gctgcggtta gtgccttttt
    27181 cacggatgat ctcggctcgt tcgatcaacg ctttatcgtt aatcagcgtc gcgccgcctt
    27241 caccgcccgc cgtgtagttt ttggtttcat ggaagctaaa gcagccaata tgaccaatgg
    27301 ttcccagtgc acgccctttg taagtggaca tcacgccctg agcggcatct tccaccacaa
    27361 acagattatg ctttttcgcc aacgccataa tggtctccat ttcgcaggcc acacccgcgt
    27421 aatggaccgg cacgataacg cgcgttttgt cggtgatcgc cgcttcaatc agcgtttcgt
    27481 cgatgttcat ggtgtccggg cgaacatcca caaaaacgat ttttgcgcca cgcagcacaa
    27541 aggcattggc ggtggagaca aaggtgtagc tcggcatgat cacttcatcg ccaggctgga
    27601 tatcgagcag cagcgccgcc atctccagcg aagcggtgca ggatggcgtc agtaacactt
    27661 tggcgctgcc aaaacgttgc tccagccact gctggcagcg acgggtaaaa ccgccatcgc
    27721 cacacagttt gccgctaccc attgccgact gcatatagtc gagttcggtt cccaccaccg
    27781 gcggtgcgtt aaatggaatc atgtgatcac ctgtataacc agtacgcggt gctttctaca
    27841 ttcgcaccac tttgtatgta tcgtttaagc gcggcggtgt tgcccatctg ggtcgccacc
    27901 cgcaaagttg ttttaccgcg agcatacgcc cagtttagcg ccgtttgcat cagctcagca
    27961 cctgcaccgc gtccagccag caggccaatt cgcgcatctg tcgcattgag ttcccgtaaa
    28021 gagacataac cgcgaatatc gccggacgcc gcacgtaaaa tcagacattg atgatcaaag
    28081 gtgccgcgca cggcattttc aatccactgt gcataaaagc gactgctggc gtcaggcgca
    28141 taccacggcg cacgaaaacg gctttgcgca aatgcggcgc tggctaactg acgtaatgcg
    28201 ggaatatcgg tctcttgtgc cactacagca ccgctatcac tgacattgtt cacgggtagc
    28261 gccaaatcaa tttcaccttc taccagggag aatcccagct gttgcagggc atccagttca
    28321 cccgtatttg atgccgcaat tttggcctgc acccgtgacc acggcgctaa cgcgtctggc
    28381 gtcaggagcg gtgcttcaga cgtaatgcgc acgatggcgc tgttaacacc aaagaaggcg
    28441 ttttcccagg ttagtggctc aatactggcg cggacgggca cgaagtaact ccagcagata
    28501 ttggccgtag ccagttttcg ctaatgaact ggcagcacgc ttcacaccct cgtcatcgag
    28561 ccacccgtta cgccaggcga tctcttccag gcaggcaatc ttaaagccct ggcgtttttc
    28621 caccgtctgt acaaaggtgc tggcttcaat caggctgtcg tgagtgccag tgtccagcca
    28681 ggcaaatccg cgcccgagca gttcaacggt caggttgccc gcctcgaggt acatctggtt
    28741 gatggaggta atctccagtt caccacgctc cgacggcttc acctgctttg cgtactccac
    28801 gactttactg tcgtagaaat aaagcccggt caccgcccag tttgacttcg gctgttttgg
    28861 cttttcttcc agcgagatag cgcggaaatt gtcgtcaaac tccaccacgc caaagcgttc
    28921 cgggtccatc acctgatagc caaaaaccgt cgccccttcc gtgcgcgccg caacatgacg
    28981 cagcttcgga ctgaagccct gaccgaagaa gatgttatcg cccagcacca gacaagaagg
    29041 ttcaccatta aggaaggttt caccgatgat aaaggcctgc gccagaccgt ccgggctggg
    29101 ctgttcggca tattccagct ggataccgaa ctcactacca tcgcccagca agcgctggaa
    29161 ataaccttta tcttccggcg tagtgatgat gagaatttcg cggataccgg ccagcatcag
    29221 caccgacagc ggatagtaaa tcattggctt atcgtaaatc ggcaacagtt gcttcgatac
    29281 gccgcgcgta atcggatgca atcgggtgcc ggaaccgccc gccaggataa tacctttcat
    29341 gtttgcctcc gctgaaaatt agcctttcag acctaaacgc tcgccctgat agctgccgtc
    29401 ctgcacctgc ttccaccagc tttcattagc cagataccac tgcaccgttt tacgcattcc
    29461 actttcaaag gtttcctgcg gcagccagcc aagttcatgg gcaattttcg aagcatcaat
    29521 ggcataacgc agatcatgcc ccggacggtc agcgacaaag gtgatcaggt cacgatagtg
    29581 cgccacgccg tgcggcttgt tcggagccag ttcttccagc agctcgcaaa tggtttccac
    29641 aacatcgaga ttcttacgct cgttgtgacc accaatatta taggtttcac cgactttccc
    29701 ggtggtcgcc acgcaataca gcgcgcgggc gtgatcttcc acatacagcc agtcacggat
    29761 ttgctgcccg ttgccatata ccggcagcga tttacccgcc agcgcgttga ggatcatcag
    29821 cgggatcagt ttttccggaa agtggtaagg gccgtagtta ttcgagcagt tggtgataag
    29881 cgtcggcaga ccataggtcc gcagccaggc gcgcaccaga tggtcgctgc tggcttttga
    29941 cgcggaataa gggctgctcg gcgcatacgg cgtggtttcg gtgaagaaat catccgtcga
    30001 gtgcaggtca ccatatactt cgtcagtgga gatatgatga aaacggaacg ctgatttttt
    30061 atcttccgtc agcgcattcc agtaagcccg cgccgcttca agcaatgtat aagtcccgac
    30121 aatgttggtt tcaataaacg ctgccgggcc gtcaatagaa cggtcaacat ggctttcggc
    30181 tgccagatgc atgacacagt ctggctgatg ctcagtgaat acgcgcgcca gttctgcccg
    30241 atcgcagata tcaactttct caaaggcaaa gcgctcgctt tgcgcgaccg gtgccagcga
    30301 catcaggttt ccggcgtagg tcagcttatc gaccactacc accgcatcgc tcgtttcgtt
    30361 gatgatataa cgcaccagcg ccgagccaat aaacccggca ccgcctgtta tcagaatttt
    30421 tctcatcagc gccagactcc tttggcatcg acgacatact gctgatggac attgtcgcca
    30481 ttgataactt tgaactggct atgatcgacc agcatcacca gcacatctgc cgttgccagc
    30541 gcctcgtcaa gctgcgccag agtacaaagc ccggtcagtt ttttcggcaa ctggtggatg
    30601 ttaggctcaa caaccagagt ttcgccgcta tgccactggg cgatcagttc agcgatttcc
    30661 atcgccgggc tttcgcgcag gtcatcaata ttcggtttaa acgccagacc aaagcaggcg
    30721 attttcagtt cactggcgcg tttatcggta gccgccaggc aatcagccac cgccgctttc
    30781 acctgatcga taacccagaa cggtttgtga tcgttcactt cgcgcgcggt acggataagc
    30841 cgcgcctgct gggggttctg tgccacgata aaccacggat caacagcaat gcagtgaccg
    30901 cccacgccag ggccaggctg aagaatatta acgcgagggt gacgattcgc caggcgaatc
    30961 agttcccaga cgttaatccc ctgatcggca caaatcagcg acaattcatt agcaaaagcg
    31021 atattcacat cgcggaagct gttttcggtg agcttacaca tttccgccgt ccgcgagtta
    31081 gtgacgacac actcaccttc gaggaaaatt ttgtacagtt cgctggcgcg ggccgaacaa
    31141 accggcgtca taccaccaat cacgcgatcg tttttaatca gctcgaccat aacctggccc
    31201 ggtaacacgc gttccgggca gtaagcaatg ttgacgtccg cctgctcgcc cacctgctgt
    31261 gggaaagtga gatccggacg catctctgct aaccattctg ccatcttctc ggttgacccc
    31321 accggcgagg tggattcaag gatcaccagc gcgccttttt tcagcactgg cgcaatggag
    31381 cgagcagccg attcaacgta ggtcatatct ggctcatgat cgcctttaaa cggcgtgggt
    31441 acagcaatca gccaggcatc cgcttcaact ggcgtcgtgc tcgctcgtaa aaaaccgcct
    31501 tctacggcag tttttactac gctcgccaaa tcaggctcga cgatatggat ttcgccacga
    31561 ttgatggtat caactgcgtg ttggttgata tcgacaccaa tcacctgttt ttgccgtgag
    31621 gcaaacgctg ctgctgtagg cagcccgatg taacccagtc cgataacaga aatggtcgca
    31681 aaactcatag tgatatccga ttatttttta acgcttccag aatgcgagag catgcctgac
    31741 catcaccata cgggttatgg gcgcggctca tagcttgata ttcgttttcg tcttttaaaa
    31801 gacgcgtcac ttcctcgaca attcgctgct tatccgtgcc taccagacgc accgtacccg
    31861 ccgtcaccgc ttccggacgc tcagtggtat cgcgcatcac cagcaccggt ttccccagcg
    31921 aaggcgcttc ttcctgaatg ccgcctgagt cggtcaaaat cagccaggcg tggttcatca
    31981 gccagacaaa cggtaaatac tcctggggat cgatcagaat gacatttttc acatgcccca
    32041 gaatgcgatt gaccggctct ctgacgttcg ggttgagatg caccggatag acaatctgga
    32101 tgtcctggtg cgtggtggct atgtctgcta gcgcatggca gatttcttca aagccacgac
    32161 cgaagctttc acgcctgtga ccggtcacca gaatcatctt tttatcgggg tcgataaacg
    32221 ggtaatttgc cgcaagttct gaacgcagcg tatcgctgct catcacctgg tcacgcaccc
    32281 ataacagtgc atcaatgact gtattaccgg taatgaagat tcggctatcc gcaacgtttt
    32341 cacgcagcaa gttttgccgg gaagtttcgg ttggagagaa gtgatacatc gccagatgcc
    32401 cggtcaatgt acggttagcc tcttccggcc acggcgaata gagatcgccc gtgcgtaaac
    32461 cagcctcaac gtgaccaaca ggaatacgct gataaaacgc cgccaggctg gttgccagcg
    32521 tcgtcgtcgt atcgccgtga accagcacga cgtctggttt gaactcggca agaataggtt
    32581 ttagcccttc cagaatccga caggttatct ctgtcaggcc ctgtcctggc tgcattatgt
    32641 tgagatcgta gtcaggtaca atggaaaaga gtttcagcac ctgatcgagc atctcccgat
    32701 gctgcgcagt gacgcaaact ttagcctcaa aaaaaggatc ttttgccaac gcatgcacca
    32761 acggagccat cttgatggct tccgggcgcg taccaaatac agtcagtact ttcacatcga
    32821 ttctcttcga ataagcggcg agcgcctttg cgctcaccgc agcagtgttg ctatttcgag
    32881 caacggcggg ttaatgcgac accagccccg atcagccccc cgacaatgcc ccacataatc
    32941 atcaggaagg cacgacgtgg gctatcgcgt tttaccggtt cttccggcgt acgcaaatag
    33001 cgataggtct gaaaacgcgg atccagggtt ggaccaacat tcagggtgtt taacatggcc
    33061 cgattctgat catagtcgag atcaaaggcc ggaccgacgg cctgtaaatt ttccagtcga
    33121 gcctggagca ttggacgccc aagcaggaac atttctgaat caggtaattc ctcggcaggc
    33181 acatctgtcg cactgcgcga aatattatgc tgctcagcaa ttttcagcgc ctgctcaata
    33241 ctgttcatcc ggcggtcgta gatggctttc gccacctctt cctgacgctt cacctgtgct
    33301 ttcatctgga tggtacgcgc cgcccatgcg cctttcagct catcattcag atggctggct
    33361 gcacgctggc tggcaaaagc aacatactga cgtaacaggt tattagcgtc aggcgcggtt
    33421 tcggcaataa gcttcacgct gtcattgacc gcgcgggtaa agtctccggg gataaactgg
    33481 atgttgttaa tcatttcatc cagcaacgcc gcatcggctt tgctgttgcc caccatccgc
    33541 tgcttgtaat agtcggtttg cagccagaac tctctgcggg tatcccacga ggccagttgc
    33601 ataacaaact ctttataggc ttcgtccatg accgatggtt ggtcggcaga agccatgttt
    33661 gaacggacat ccaggttacg caaaaattgc tgctgcgagt aatatccccc cagcatattc
    33721 accgttggac gatcggtaat cgccgtcgag ctccactcct gacgagcaaa aaaagtatac
    33781 gccagcgcga ttaacgcaaa cgccagcccc atgccaataa tccatagctt cccagcccac
    33841 aaggtacgaa acaacccacg aatatccagt tcattttcag cgtcttcggc cggtttccca
    33901 ggcattggtt gtgtcatcac atcctcattt atttggttaa attggggctg ccaccacgat
    33961 ttctacgcag tctgcgtttt acgcgcttaa taaagcgagc aactttccag gcacgcttaa
    34021 tgcaatatcc atagaggaag aatgctagca aaaagagcac cagcatgacc cactccggga
    34081 caaaatgaga atattctgcc agcacgccaa tggaagcgag cagtgctgcg gcaagggtaa
    34141 tcagcacaaa cgcctggcgg gaagtaaacc cggcacgcat gatcaaatgg tgaatatgct
    34201 gacggtcagg agagaatggg ctcatgcctt tacgcaggcg acggtacata atcgccacca
    34261 tatccattag cggaatggcg attatccaca aagcggtaac cgggctgatg ggatgggttt
    34321 tgccctgggt cgtttcgagc aggatccaga taacggtaaa accaatcagc gtactgcccg
    34381 catcacccat aaagactttg tagcggcgac ccaggatacc aaggttaagc atgatgtatg
    34441 gcaggatggc ggcgatcatc gcaaagcacc agattgcgag gctggtttgc ccgtcgaacc
    34501 acaaaatcat accgattgct gcaaacgaga cgcaggacaa cccgcccagc aagccatcaa
    34561 tgccatcaac catgttgaac gcattaatgg ccgcccagac ggcaaatagc gtcaggaagt
    34621 aaccaaacgg tccgagcacc atctcccagg agccaaagat ataacccagg ctactgagat
    34681 aaagcttgcc gaacaccatc ataacaatgc caacagcggc ctgtatggtg gcacggattt
    34741 ttacgctgat atcaaaacgg tcatccagcg cgccaatgaa aacaagcaca ccggcacaag
    34801 cgagatagag agatgcatgc ggaatatagt aatcgacaat tccgaacgtg aagcaaatcc
    34861 ctgcgtaaac cgaaatcccc ccaacgagag gtatcaatcc ctggtgacgt ttgcggaagt
    34921 ttggtttatc cactaaaccg actttttttg ccaccttacg ggcaaaaaac agaaacagtg
    34981 tcgtgaataa aaaaatactg atgagatcag tactcactgt cagtaaattc acaatgcatg
    35041 ctctcagaga aaattattag cagaagtata accacgaaga cctttattca gaagggaaag
    35101 tccgttccga acccaccagt cctgtgcaaa aattaaccat ttgatatgca aattgcataa
    35161 catgccagca aattccagga attttcctaa catcgccagc gcggcattaa gattacagat
    35221 ataaaagcaa aacgccacgt aaacacgtgg cgtttttggc ataagacaaa tttatgagcg
    35281 tttcatcatt tcgaagaaat cgtcattggt cttggtcatt gccagtttat taatgaggaa
    35341 ttccattgca tcgatttcac ccatcgggtg aatgattttg cgcaggatcc acattttctg
    35401 cagttcttcc tgagtcgtga gcagctcttc tttacgggta ccggaacggt tgtagtcgat
    35461 agccgggaag acgcgttttt cagcgatctt acgagagagg tgcagttcca tgttgcctgt
    35521 acctttaaac tcttcgtaaa taacttcgtc cattttagaa ccggtatcga taagcgccgt
    35581 cgcgataatg gtcaggctgc cgccctcttc cacgttacgc gccgcaccga agaagcgttt
    35641 cggacgatgc agggcgttgg catccacacc accggtcaac actttacctg acgccggaac
    35701 aacggtgttg taagcgcgcg ccagacgagt gatggagtcg agcagaatga taacgtcttt
    35761 cttgtgctca accaggcgtt tcgccttctc gatcaccatt tccgcaacct gaacgtggcg
    35821 agatgcgggt tcgtcaaagg tagaagcaac aacttcacct tttaccagtc gctgcatctc
    35881 ggttacttct tccggacgtt cgtcgatcag cagaaccatc agcacacaat ccgggtggtt
    35941 gtaagcaatg ctctgagcaa tgttctgcag cagcatggtt ttaccggctt tcggcggtgc
    36001 cacaatcaga ccacgctgac cacgaccgat aggtgatgcc agatccagta cgcgagcagt
    36061 taaatcttcc gtagaaccgt taccacgttc catacgcaga cgagagtttg cgtgcagcgg
    36121 ggttaagttc tcaaagagga ttttgttgcg ggcgttttca ggtttgtcga agttaacttc
    36181 gttaactttc agcagcgcaa aatagcgttc accttctttc ggcgggcgaa tcttaccaga
    36241 gatggtatca ccagtgcgga ggttgaaacg gcggatttgg ctaggggaaa cgtagatgtc
    36301 atcaggaccg gcgaggtagg agctgtctgc ggaacggagg aaaccaaatc catcctgcaa
    36361 tatctccagt acgccatcac caaagatatc ttcgccactc tttgcgtgct gcttcaggat
    36421 ggcaaaaata atgtcctgct tacgcatacg agccaggttt tccagcccca tattttcgcc
    36481 gagagtgatc agctcagaaa ccggcgtatt ctttaattcg gtaagattca tagtggtgtg
    36541 agttcttaaa cttggggtaa atctcgaact taatgttgtg aatggtatgg caggatcatc
    36601 catgcctgtt tacggtcatc gactcatgtc ttttcgctgc ctggtcacag gagagtacgc
    36661 agaactgaaa cgacaagacg gattgagtga cgagccagaa atctgttcac ttcgcattta
    36721 agataaaacg ggaagcgttg gaaattacaa gattcaaact taataaggta tgtttaatac
    36781 gaagtcaaca ctaagttagc atgactcacg ccgggcgtcc agtttttagc gacggggcac
    36841 ccgaacatga aattccctta cgccaggtta gcgtcgagga actctttcaa ctgaccttta
    36901 gacagtgcgc ccactttggt tgccgccact tcaccgtttt tgaacagcag cagagtcggg
    36961 ataccacgga tgccatattt cggcgcagtg ccagggtttt gatcgatgtt cagttttgca
    37021 acggtcagtt tgccctgata ttcgtcagcg atttcatcca gaatcggggc gatcattttg
    37081 cacggaccgc accactctgc ccagaaatcg acgaggatcg ccccgtccgc tttgagtaca
    37141 tccgtgtcaa aactgtcgtc agtcaggtga ataattttat cgctcatata taactccaca
    37201 ggaataagcc tggcgtgttg gtttcgttgt tggtgtaaca ttaaccaact aaaggttgac
    37261 tttatttcac cggatacgct ttcgtaaagc aatagtaagc tgatattcta ccacactatg
    37321 agcaaaacac atttaacaga acagaagttt tccgacttcg ccctgcatcc gaaggttgta
    37381 gaagcccttg aaaaaaaagg gtttcataac tgtacgccca ttcaggcact ggcccttccg
    37441 ctgacgctgg cgggtcgtga cgtagccggg caggcgcaaa ccggtaccgg gaaaacgatg
    37501 gcgtttctta cgtcaacgtt tcattatctt ctctctcatc ctgcgattgc cgatcgcaag
    37561 gtgaatcagc cgcgtgcctt aattatggca ccgacgcgtg aacttgccgt gcagatccat
    37621 gccgatgcgg aaccgctggc ggaagctacc ggcctgaagc tgggtctggc ttacggtggt
    37681 gatggttacg acaaacagct gaaagtgctg gaaagcggcg ttgacattct gattggcacc
    37741 acgggtcgtt taattgacta cgccaagcag aaccacatta acctcggtgc cattcaggta
    37801 gtggtactgg acgaagccga tcgcatgtac gatctgggct ttattaaaga tatccgctgg
    37861 ctgttccgcc gtatgccgcc tgcaaaccag cgcctcaaca tgctgttctc cgccacgctt
    37921 tcgtaccggg tacgtgaact ggcgttcgag cagatgaaca atgccgaata tattgaagtg
    37981 gaaccggaac agaaaacggg ccaccgtatt aaagaagagc ttttctaccc ttctaacgaa
    38041 gaaaaaatgc gtttgctgca aacgctgatc gaagaagagt ggccagacag agcgattatt
    38101 ttcgccaata ccaaacaccg ttgtgaagag atctggggcc acctggcggc agatggtcat
    38161 cgtgtcggtt tgttgacagg cgatgtcgcg cagaaaaaac gtctgcgtat tcttgatgaa
    38221 tttacccgtg gcgatctgga tattctggtt gccaccgacg ttgccgcgcg tggtttgcat
    38281 attccggcag tgacgcacgt ctttaactac gatttacccg atgactgtga agattacgtt
    38341 caccgtattg gtcgtacagg tcgcgcaggc gcaagcggtc actctatcag cctggcgtgt
    38401 gaagagtatg cattgaattt gcctgctatt gagacctata ttggtcactc aattccggta
    38461 agcaaataca atccggacgc attgatgacc gatctgccaa aaccgctgcg cctcacgcgc
    38521 ccacgtacag gcaatggtcc gcgtcgtact ggcgctccgc gtaatcgtcg tcgttcaggt
    38581 taaataaaat gtcatactca aaatcattca aactgtatca agatggcaag agaatgaatc
    38641 ctgaatcact tactgtagta agtgattcag gtgaaagaat gcagccaaca cagagacaga
    38701 ttgaaggatg aagagtatgg gttccgcctc gtcgctgtat gcagccattg atctcggttc
    38761 gaatagtttt catatgctgg ttgtgcgcga ggtggctgga agcatccaga cgctgacgcg
    38821 aataaaacgc aaagtgcgtc tggctgctgg cctgaatagc gaaaatgccc tctccaatga
    38881 agcaatggag cgcggttggc aatgtttgcg cctgtttgct gaacgtctgc aagatattcc
    38941 tccctcgcaa attcgcgttg tcgctacggc gacgttacgc cttgccgtca atgcgggtga
    39001 ttttattgcc aaagcgcagg aaattctcgg ttgtccggta caggtgatca gcggtgaaga
    39061 ggaagcacgt ctgatttatc agggcgttgc tcacaccact ggtggtgccg atcagcgcct
    39121 ggtggtggat ataggcggag ccagtactga actggtaacc ggcacgggtg cacaaaccac
    39181 ctcgttgttc agcctgtcga tgggctgcgt cacctggctg gaacgctatt ttgccgatcg
    39241 taatctgagg caggaaaatt ttgatgctgc agaaaaagcg gcacgcgaag tgttacgtcc
    39301 ggttgccgat gaattacggt atcacggctg gaaagtgtgc gttggtgctt ccggcaccgt
    39361 gcaggcgtta caggaaatca tgatggcaca ggggatggat gaacgcatta ccctggaaaa
    39421 gttgcagcaa ttgaaacagc gagccattca ttgcggtcgg ctggaggaac tggagattga
    39481 cgggctgacg ctggaacgtg cgttagtgtt cccgagtggt ctggcgatcc tgatcgccat
    39541 ttttaccgaa ctgaatattc agtgtatgac cctggcgggc ggtgcgctgc gtgaaggcct
    39601 ggtctacggc atgttgcatc tggccgtcga gcaggatatt cgcagccgta cgctgcgtaa
    39661 tattcaacgc cgctttatga tcgatattga tcaggcacag cgcgtagcca aagttgcggc
    39721 taacttcttc gatcaggtgg aaaacgaatg gcatcttgaa gcaataagcc gcgatttgct
    39781 catcagcgcc tgtcagcttc atgaaatcgg cctgagcgtt gacttcaaac aagcgccgca
    39841 acacgctgct tatctggtgc gtaatctgga tcttcccggt tttacccccg cacagaaaaa
    39901 attgctggcg acgctactgc tcaaccagac taatccggtc gatctctcat cgctgcatca
    39961 gcaaaatgcc gtaccgccgc gcgtcgcaga acaactctgc cgtttactac gcctggccat
    40021 catttttgcc agccgtcgcc gtgacgatct cgtgccagag atgacattac aggctaacca
    40081 tgaactgttg accttgacgc ttccgcaagg ttggctaacc caacatccgc tgggtaaaga
    40141 gattattgct caggaaagcc agtggcagag ctatgtccac tggccgctgg aagtgcatta
    40201 atgagtaagt gccggatgcg atgctgacgc atcttttccg gccttgatta tttccctcgt
    40261 tttgccgcca tcatcgcttt cagattcgcc agatggcttt gccctttctg catccgttct
    40321 tcggcgctga ccactttgcg ctccttttcc caaatcagat catcctgcgg cagctccagc
    40381 aaaaagcggc tcggctccgg gcgcaccaat tcgccgtact gacggcgttc tttacacagc
    40441 gtaaaggtca attccttctg ggcgcgggta atgccgacat aggccagccg ccgctcctca
    40501 tcgatattat cttcatcgat gctgctctgg tgcggcaaaa acccttcttc cataccgacc
    40561 atgtagacat aaggaaactc cagccctttc gacgcgtgga gagtcatcag ttgcacctga
    40621 tccagctctt cttcactctc actacgctcc atcatgtcgc gcaaagtaaa gcgcgtcacc
    40681 acctgggtga gcgtcatcgg ctcatccagt tcactgcctt ccagcatctc cgtcatccag
    40741 ctaaacagtt ggttgacgtt cttcatgcgc atttcggcgg ctttcgggct gggcgatgtt
    40801 tcgtacagcc aggattcata atccatgcca tggatcagat cacgcaccgc ggcaatcggc
    40861 tcccgctccg ccagacgctg gatttctgcc aaccagtgag tgaagcgggt caatgcttca
    40921 taaccacgtc cgctaagcgt ctgactcagg cccatatcaa agctggcggt aaacatgctt
    40981 ttattgcgcg tcatcgccca ttcacccaac tttttcagcg tcgccgggcc aatctctcgc
    41041 ttcggcgtgt taacgatacg cagaaatgcg ctgtcatcgt ccgggttagt cagcacgcgc
    41101 agataagcca gcaagtcctt gatttcagga cgagagaaaa acgacgtacc accggatatt
    41161 ttgtacggga tgcggttttg catcaggaat ttttcaaata cccgcgactg atggttgccg
    41221 cgataaagaa tggcataatc tttgtactgc gttttattga cgaagtgatg ggcgatcagc
    41281 tcgccagtaa cgcgctcggc ttcatgttcc tcgttattcg cgcttaaaac ttttagctct
    41341 gtgccgtaac ccagttcgga gaacagacgc ttttcaaaga cgtgcgggtt attggcgatc
    41401 aggatgttcg ccgctttcag aatacgcccg gaagagcgat agttctgctc aagcttaatc
    41461 accttcagcg ccggaaaatc ctgactcagc agcaccaggt tttgcggacg tgcaccgcgc
    41521 caggagtaga tcgactggtc atcgtcaccc accacggtaa agcgcgcgcg gctgcccacc
    41581 agcagtttca ccagttcata ctggctggtg ttggtgtcct gatactcatc caccagcagg
    41641 tagcgaattt tgttctgcca gcgctcgcgg acttcttcat tgcgttgcag cagcaacgtc
    41701 ggcaataaaa tcagatcatc gaaatcgaga acgttgcagg ctttcaggtg tgcatcatac
    41761 agcccataac aatgggcaaa aatacgttcc cgctcgccct ttgcctctgc cgccgcctgc
    41821 gccggtgttt tgagatcatt cttccagtta gagatggtcg aaatcagttg ttgcaggaga
    41881 actttgtcat cttcaatcag cccctcggtc aactctttaa gcaaagcaag ctgatcggta
    41941 tcgtcaaaca acgagaagtt cgctttcatc ccaagcgccg catactcgcg tttgatgata
    42001 tccagcccca acgtatggaa agtggagatc atcagcccac gcgcctcttt gcgccccagc
    42061 gtctgcccta cacgctcttt catctcgcgc gccgccttat tggtaaaggt caccgccgcg
    42121 atatgtcgcg cctgataacc gcaaccgcgg atcagatggg cgattttatt ggtgatcaca
    42181 cgagttttac cggaacccgc gcccgccagc accaggcagg ggccggtaac gaattcgaca
    42241 gcttgttgtt ggccggggtt tagacgcata ggtgtattgc tcaatcttcg aacgggggga
    42301 ggattgtagc agaatcgcat ttgaatgtga ccgcgtgtaa gccgctaacc ggttggctgc
    42361 ggatgtttaa atcagagaga gaagaccgaa atcggagttc atcgtgaatc cattagactt
    42421 agaaaaaatc gggtcgtaac gttgtgttat tactttttca gagttcacta aggagaggtt
    42481 tactcgtggc gattaatttc agccctaagg ttggtgaaat actggaatgc aactttggta
    42541 actatcctgt tagccaaaat ggaccgttca gcacgacgta ttacgatggc cgcattcctc
    42601 ctgaaatgat aaaaaacagg ttagttgtcg tgctcaacgg aaaaattaat ggcaacgcat
    42661 gtatcgtcgt accattatca accactcgcg accatgacaa gttaaatcgt ggaatgcatg
    42721 ttgaaatagc cagtaatatt atcaatgacc tgcaattttt tgatcagcag attcgttggg
    42781 ccaaagccga cctggtacaa caagtcagtc gaaacagatt aaacagagca agaacttaca
    42841 gagggtatct taatcaatgt ttgccacatg agttggttgc agacatacag agggcggtca
    42901 tcaagtcaat caatgccata tcgctcataa attgacaata aaatcaccat ccccgataat
    42961 gactttgtag cccatagagg ccctaagttt tctccccttg agggacctgc gaagcccgga
    43021 ttttccgggc ttttgccata tatgtaccag ccactccctg actgacaatt acgttagcat
    43081 ctgcggatca tgctaaaatc gccgcctgac aattattcac tcaaggcacg atcatggcaa
    43141 aaacagcagc agcactgcat atccttgtaa aagaagagaa actggctctg gatcttctcg
    43201 agcagattaa gaacggggcc gatttcggca agctggcgaa gaaacactcc atttgcccat
    43261 caggcaaacg cggcggtgat ttaggtgaat tccgccaagg tcagatggtt ccggcgttcg
    43321 ataaagttgt gttctcttgt ccggtactgg agccgactgg cccgctgcat acccagttcg
    43381 gttaccacat cattaaggtg ctttaccgta actaacagta aggccttctc caggagaagg
    43441 ccttgaactc tgaatcgcta ttaacccgca acagcaatgc gtttcatatc tgtcatatag
    43501 ccgcgcagtt tcttacctac ctgctcaatc gcatggctgc gaatcgcttc gttcacatca
    43561 cgcagttgcg cgttatctac cgcgccttcc ggaatagctt tacccaggtc gcccggttgc
    43621 agctctgcca taaacggttt cagcaacggc acacaagcgt aagagaacag atagttaccg
    43681 tactcggcgg tatcagagat aaccacgttc atttcgtaca gacgcttacg ggcgatggtg
    43741 ttggcaatca gcggcagctc gtgcagtgat tcataatatg cagactcttc aatgatgccg
    43801 gaatcgacca tggtttcgaa cgccagttca acacccgctt tcaccatcgc gatcatcagt
    43861 acgcctttat cgaagtactc ctgctcgccg attttgcctt catactgcgg cgcggtttca
    43921 aacgcggttt tgccggtctc ttcacgccag gtcagcagtt tcttatcatc gttggcccag
    43981 tccgccatca tgccggagga gaattcgccg gagatgatgt catccatatg tttctggaac
    44041 agcggtgcca tgatctcttt cagctgttca gacagcgcgt aagcacgcag tttcgccggg
    44101 ttagagagac ggtccatcat cagggtgatg ccgccctgtt tcagcgcttc ggtgatggtt
    44161 tcccagccaa actgaatcag tttttctgca tatgccgggt cggtgccttc ttccaccagc
    44221 ttgtcgaagc acagcagcga gccagcctgc aacataccgc acagaatggt ttgctcgccc
    44281 atcaggtcag atttcacttc cgcaacgaag gacgattcca gcacgcccgc acggtgacca
    44341 ccggttgctg ccgcccatgc tttagcgatc gccatacctt cgcctttcgg atcgttttcc
    44401 gggtgaacgg caatcagcgt cggtacgccg aatccacgtt tgtactcttc acgcacttcg
    44461 gtgccagggc atttcggcgc aaccatgact acggtgatat ctttacggat ctgctcgccc
    44521 acttcgacga tgttgaaacc gtgcgagtaa cccagcgccg cgccgtcttt catcagtggc
    44581 tgtacggtgc gcactacatc agagtgctgc ttgtccggcg tcaggttaac caccaaatcc
    44641 gcctgtggga tcagttcttc gtaagtaccc actttaaaac cattttcggt cgctttacgc
    44701 caggacgcgc gcttctcggc aatcgcttct ttacgcagag cgtaggagat atcgagacca
    44761 gaatcacgca tgttcaggcc ctggttcaga ccctgtgcgc cacagccgac gatgactact
    44821 tttttaccct gaaggtagct cgcgccatcg gcgaattcat cgcggcccat aaagcgacat
    44881 ttgcccagct gtgccagctg ctggcgcaga ttcagtgtat tgaagtagtt agccatggtg
    44941 attcctcgtg atgttgtgct tcttattgtt cggttcgcgt ttgcgagatt gaattcacta
    45001 tatgacagga aatttattgc ggaaattgat atattcacaa cgtcacattg caatttttgc
    45061 aacgtcaaca tcgagggctg tccctgtgga tttacgcgat ctgaaaacct tcctgcatct
    45121 ggcggaaagc cgccattttg gccgcagcgc gcgggcgatg cacgttagcc catccacgct
    45181 ctcacggcag attcagcgcc tggaagaaga tctcggtcag ccgctatttg tgcgcgataa
    45241 ccgcacggta acgctgacgg aagcgggcga agagctgcgc gttttcgccc agcaaacgct
    45301 gttgcagtat cagcagttgc gccacaccat cgatcagcaa gggccgtcgc tctctggcga
    45361 attacatatc ttctgctcgg tgacagctgc ctacagccat ctgccgccga ttctggatcg
    45421 cttccgcgcg gaacacccgt cggtggagat taaactcacc actggtgatg cggcagatgc
    45481 aatggaaaag gtggtcactg gcgaagcgga tctggcgatt gccgggaaac cggagacgct
    45541 acccggcgct gtggcgtttt cgatgctgga gaatctggcg gtagtgctga ttgcgcccgc
    45601 gctgccctgc ccggtgcgta atcaggtctc ggtagagaag ccggactggt caacggtacc
    45661 gtttatcatg gccgatcagg ggccagtacg ccgccgcatt gaactgtggt ttcgacgcaa
    45721 taaaatcagc aacccaatga tttacgccac ggttggcggg catgaagcga tggtatcgat
    45781 ggtggcgctc ggctgtggtg tggcgctgtt gccggaagtg gtgctggaaa acagcccgga
    45841 gcctgtacgc aaccgggtaa tgattttaga tcgcagcgat gagaaaacgc cgtttgaact
    45901 gggcgtgtgt gcacaaaaaa agcggctgca tgagccgctg attgaggcgt tctggaggat
    45961 tttgccgaac cacaaatgac gttgtcgcgc gagtaggcct gataagcgaa gcgctatcag
    46021 gcatttttcc ctaacccgcc aaaaagaacc tgaacgccgg gttattggtt tcgtcgtggc
    46081 aatcgtagcc cagctcattc agccgggttt cgaaatccgg ttcatggtcg ccaagttcga
    46141 acgccgccag tacgcgcccg tagtcggtgc catggctgcg atagtggaac aaagaaatgt
    46201 tccagtgcgt acccagcgtg ttgaggaagc gcagcagcgc gcccggtgat tccgggaatt
    46261 cgaagctgta gaggcgttcc tgcaacggat gtgacggacg cccgccgacc atatagcgca
    46321 catgcaactt tgccatttcg tcgtcggaga gatccaccac gctgtagccg ccgtcgttga
    46381 gcatctgcaa aatttctttg cgctcttcga ggccgcggct caggcgcacg ccgacaaaga
    46441 tgcaggcgtt tttggcatcg gcaaaacggt agttgaactc ggtgaccgat cgcccgccaa
    46501 gcagttggca gaatttgagg aagctgcctt tttcttccgg aatggtcacc gccaacaacg
    46561 cttcacgctg ttcgcccagt tcgcagcgtt ccgagacgta gcgcaggccg tggaagttca
    46621 cgttggcacc ggaaagaata tgcgccagcc gttcgccgcg aatgttgtgc tgggcgatat
    46681 attttttcat tcccgccagc gccagcgcgc cagagggttc cgccaccgca cgcacatctt
    46741 cgaataaatc cttcatcgcc gcacagatcg catcactatc gacggtgatg atatcgtcga
    46801 gatactcctg gcacaaacgg aaggtttcgt cgccgatgcg ttttaccgca acgccttcag
    46861 caaacagccc cacgcgcggc agatcaaccg gatgacccgc atccagcgct gctttcaggc
    46921 aggcggagtc ttccgcttct acggcgatca ctttgatttg cggcatcagt tgtttgatca
    46981 gcaccgccac gccagcagcc agaccgccgc cgccgactgg cacaaatacg cggtcgagat
    47041 gggcgtcctg ctggagcagt tccagcgcca gcgtgccttg cccggcaatc accatcggat
    47101 ggtcgaacgg cggcacccag gtgaagccct gctgctgtga cagttcgatc gctttagctt
    47161 tcgcttcgtc aaagttcgcg ccatgaagca gcacttcgcc gccgaatcca cgtaccgcat
    47221 cgactttgat gtcggcggtg gcggttggca taacgatcag ggccttcacg cctaaccgtg
    47281 cggaagaaaa cgcgacgccc tgcgcgtggt tacccgcaga agcagtgatc acaccgtgcg
    47341 ctttttgttc ttccgtcagg cccgccatca tggcgtatgc gccgcgcagc ttaaagctgt
    47401 gcactggctg gcgatcttcg cgcttcacca gaatgacgtt atcaagacgc gacgacagtt
    47461 tttccatttt ttgtagcggc gtaacctgcg ccgcctcgta aaccggcgcg cgcagcactg
    47521 ctcttaaata ttcggcacct tccggagcac cggacagggg ttgcgagtca gccattatta
    47581 accccccagt ttcgatttat cgcgcaccgc gcctttgtcg gcgctggttg ccaggctggc
    47641 ataagcacgc agggcaaagg agacctgacg ttcacgattt ttcggcgtcc aggctttgtc
    47701 gccacgggct tcctgcgctt cacgacgcgc cgccagttcg gcatcgctta cctgtaactg
    47761 aatgccacgg tttggaatgt cgatggcgat caggtcgccg tcttcaatca ggccaatgct
    47821 gccgccgctt gccgcttccg gtgagacgtg gccgatagaa aggccagagg tgccgccaga
    47881 gaaacgaccg tcggtgatca gcgcacaggc tttaccgagc cccattgatt tcaggaagct
    47941 ggttgggtag agcatttcct gcatccccgg accgcctttc gggccttcgt agcggataac
    48001 aaccacatcg cccgcgacaa ctttaccgcc gagaatcgct tctaccgcat cgtcctggct
    48061 ttcgtatact ttcgccgggc cggtgaattt gaggatgctg tcatcgacgc ccgcggtttt
    48121 aacgatgcag ccgttttccg cgaaatttcc gtagagcacc gccaggccac cgtctttgct
    48181 gtaggcgtgt tccagcgagc gaatacagcc atttgcgcga tcgtcgtcca gcgtatccca
    48241 gcggcaatcc tgcgagaatg cctgtgtggt acgaatgcct gcagggcctg cgcggaacat
    48301 attttttacc gcgtcatcct gggtcagcat aacgtcgtat tgttccagcg tttgcggcaa
    48361 cgtcaggcca agtacgtttt tcacatcgcg attcagtaag ttagcgcgat ccagttcgcc
    48421 gagaataccg ataacaccac cagcacggtg aacatcttcc atatggtatt tttgggtgct
    48481 cggcgcaact ttgcataact gagggacttt acgcgaaagt ttgtcgatat cactcatggt
    48541 gaagtcgatt tccgcttcct gcgccgccgc cagcaggtga agtacggtgt tagtcgatcc
    48601 acccatcgcg atatccagcg tcatggcatt ttcaaacgcc gctttgctag caatattgcg
    48661 gggcagtgca ctttcgtcgt tttgctcgta gtaacgtttg gtcaattcaa caatgcgttt
    48721 accagcattg aggaacagct gcttacggtc agagtgggtt gccagcagcg agccgttgcc
    48781 cggctgcgac agacccagcg cttcggtcag gcagttcatt gagttagcgg taaacatccc
    48841 ggagcaggaa ccgcaggttg ggcatgcgga acgttcaacc tgatcgctct gggagtcaga
    48901 aactttcggg tctgccccct ggatcatcgc atcaaccaga tcgagcttga tgatctgatc
    48961 ggacagtttg gttttcccgg cctccatcgg gccgccggaa acaaagatca ccggaatatt
    49021 caggcgcaga gaggccatca gcatccccgg ggtgattttg tcacagttag agatgcagac
    49081 catggcgtcg gcgcagtggg cgttgaccat atactcaacg gaatcagcga tcagttcgcg
    49141 agatggcagt gaataaagca tgcccccgtg gcccatggcg atcccatcat ccaccgcaat
    49201 ggtgttgaac tctttggcaa cgccgccagc tgcttcaatt tgttcggcga ccagtttacc
    49261 gagatcgcgc agatggacgt gacccggcac aaattgggtg aacgagttca caaccgcgat
    49321 aatcggctta ccgaaatcgg cgtcggtcat tccggtggcg cgccacagcg cacgagcacc
    49381 cgccatatta cgaccatgag tggtggtggc ggaacggtac ttaggcatac tttatttact
    49441 cccagtgtct gtctcgtaaa tgggacggtg cgtgccgtcc cattttttgt atttattgat
    49501 taacttgatc taaccagccc catttatctt cggtttcgcc ggtaaagagg ccgaagaagg
    49561 cttgctgaat gcgtttggtg accgggccac aacggccttc gccaacctga ataccgtcta
    49621 cactgcgcac tggcgtgatt tctgccgccg taccggacat aaagacttca tccgccaggt
    49681 acagggattc gcgcgacagc acctgctcac gcacttcaat tcccagctct ttcgccagtt
    49741 tgatgagggc atcacgggta atacccggca gcgcggagga ggtgaacggt ggggtgaaca
    49801 gcacaccatc tttcacttca aacaggtttt cgcctgcgcc ttcagagatg taaccgttca
    49861 catccagcgc gataccttcc tgataaccgt ggcggcgtgc ttcgctaccc accagcaggg
    49921 aagagaggta gttaccaccg gctttcgccg cggttgggat ggtgtttggt gctgcgcgat
    49981 tccaggagga aaccatcgca tcgatcccct gctccagcgc ttctgcgccc agatacgccc
    50041 cccacgggaa agcggcgata atcacatcgg ttgagtatcc cgctggcggg tttacaccca
    50101 tgccaacatc accaacgaag atcagcggac ggatataggc gctggtgaga ttgtttttgc
    50161 ggatcacgtc gcgacaagct tccatcagct catcaatgct ctgcgaaacc gggaagcgat
    50221 agattttggc ggagtcatgc agacgctgca tatgctcacg atggcggaat acaaccggtc
    50281 ctttgtgcga gtcgtagcaa cggatgcctt caaaaaccga ggtgccatag tgcagcgcgt
    50341 gcgacatcac atgcaccttc gcgtcttccc agcgaaccat ctccccattg aaccaaatgt
    50401 aatcagcttt cttcgtggtc atttttatat tccttttgcg ctcaggcgcg gatttgttgt
    50461 gatgtggttg tgctctggca gatggcaacg tgtgcgacgt ccaccagttt atttaactga
    50521 ctaaacagta agtcgaccga ccgtgggctg gcaacggtca attcgatatt tatattttgt
    50581 gcatcgctgg cggcggccat attcattgag cagacgtgga aaccacgatg acgcaccacg
    50641 cgtaaaacac gttctaaggt ttccggattg aagcgagccg atacattgac ctgatgttgc
    50701 atcatgataa tttctccaac atttctgaat tactggcacc aggcggcacc agcggccaga
    50761 cgttctcaag ttcgtcgatt gagacatgaa gcaggtatgg cccatcactg ttcagcatgg
    50821 tgtcgagtgc cgcttcaacc tggtctttac gggtgatgtg ttggccagag atgccgaagg
    50881 cgctggctaa catgagaaaa tcggggttat cggtaagggt ggtttcgcta tatcgttcct
    50941 ggaaaaacag ttgctgccat tgtcgaacca tccctaaccg ttggttatcg agtaagacga
    51001 ttttcaacgg taactgctta cgttttacgg tgcccagctc ttgtacattc atcatgaaag
    51061 agccgtcacc ggagatacag acgacggtat cgttcggtcg cgcgacttgc gcgccaaccg
    51121 ccgccggtaa accgaaaccc atggtgccta agccgctgga ggtgatgaaa ttttccgggc
    51181 gagtatgggc gatgtgctgc gcagcccaca tctggtgctg ccccacatct gtggtcacga
    51241 cgcaatccgc aggtttacga tccgacagtt gttttaacaa caacggcgcg tagatagcgt
    51301 caccgggatg gtcgtaacgc caggcatgtt catcacgcag ctgcgcgcag tgttgctgcc
    51361 agtcattgat atttaacggc tgctgtaatg ctggtaacag agcatttaaa tcaccctgta
    51421 atgccacatg tgcctgacgc agcttgttca tttctgccgg gtcgatatcc atatggataa
    51481 cactggcgtg tggtgcgaag gtgttcagtt tgccggtcac ccggtcatca aaacgtgcac
    51541 ccacggcgat cagcaagtcg cactcctgca ccgcgaagtt cgccgctttg gtgccatgca
    51601 ttcccagcat gcccagatag tacggataat ctgcttcaac tgcgcccagc cctttcagcg
    51661 tgcaggtggc aggcattttt gtggtagcga gaaattctcg taaagcagga actgcctgcg
    51721 ccatacccac gccaccacca acgtacagca tcggtttttg cgcttttgcc agcatctggc
    51781 gcgcttgctc aacttcggca tgtgggaaag tcacttcgtt ttcaacggtg gtgaaccagg
    51841 gttccaggtc accgctggct aactggatat cttttgggat atcgaccaga accggaccag
    51901 gacgacccga gatggcaact tcgaatgctt cagccatgat gcgcggcaac tcttccagcg
    51961 actgtaccag gaagctgtgt ttggtacagg ctaacgacaa tcccaggaca tccacttcct
    52021 gaaatgcgtc ggtgccgata aacggtgcgg acacctgacc ggtgatggca acgacaggga
    52081 tggaatctaa cagtgcgtcc gcaagcccgg ttatcaggtt ggttgcgccc ggaccagacg
    52141 tggcgataca tacgccggtt ttgccggtag cgcgggcata gccaatagcc gccattgctg
    52201 cgccctgctc atgtcggcac agcaagtgct ccacgccgcc gtcatacaat gcatcgtaaa
    52261 ccggcataat tgcgccaccc ggataaccga aaacggtgtt cacaccctgt gcccgcaacg
    52321 catgtaccac ccactgtgcg ccattcatag ttagttcccc gtcctgaatc ttgagaaaca
    52381 gaattttgtg ctgttattca ttgtctgctc ctcggttatg tttttaaggt caaaaaaaac
    52441 ccccggacct ttcggtgcgg gggtcttagt tcgttaaggc ttgatctcta agcctttcct
    52501 cgtccaagtg cagccccgca cggtgggata ataatcacca ccacgctaat cacgaccagg
    52561 ctaatcactc gtagaagggc tgtcattttg tcttttcttg catcttgttc gaaggaatgc
    52621 ctaaagagtt accatagatt ttgcgaatag cacaagatat t
//
LOCUS       DAPGOF010000008        51944 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.008, whole genome shotgun sequence.
ACCESSION   DAPGOF010000008 DAPGOF010000000
VERSION     DAPGOF010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51944
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.008"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            56..1495
                     /gene="norV"
                     /locus_tag="RHV13_000403"
     CDS             56..1495
                     /gene="norV"
                     /locus_tag="RHV13_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HDV1239965.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVEEEASATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            1492..2625
                     /gene="norW"
                     /locus_tag="RHV13_000404"
     CDS             1492..2625
                     /gene="norW"
                     /locus_tag="RHV13_000404"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HDV1239966.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLHLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRVLMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTESQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPV"
     gene            complement(2950..5202)
                     /gene="hypF"
                     /locus_tag="RHV13_000405"
     CDS             complement(2950..5202)
                     /gene="hypF"
                     /locus_tag="RHV13_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HDV1239967.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLRGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVAEGLPDAARQLLTTPAAPIV
                     LVDKKYVPEICDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALEDLQGIADGFLIHNRDIVQRMDDSVVRESGEMMRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMHGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(5355..5882)
                     /gene="hydN"
                     /locus_tag="RHV13_000406"
     CDS             complement(5355..5882)
                     /gene="hydN"
                     /locus_tag="RHV13_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HDV1239968.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(6031..7041)
                     /gene="ascG"
                     /locus_tag="RHV13_000407"
     CDS             complement(6031..7041)
                     /gene="ascG"
                     /locus_tag="RHV13_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HDV1239969.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHSIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKVLHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            7301..8758
                     /gene="ascF"
                     /locus_tag="RHV13_000408"
     CDS             7301..8758
                     /gene="ascF"
                     /locus_tag="RHV13_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HDV1239970.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDALIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMLLEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSAQPTVAKEVSLN"
     gene            8767..10191
                     /gene="ascB"
                     /locus_tag="RHV13_000409"
     CDS             8767..10191
                     /gene="ascB"
                     /locus_tag="RHV13_000409"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HDV1239971.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     ELTPNQQGIAFYRSVFKECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDSLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGTYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(10305..10724)
                     /locus_tag="RHV13_000410"
     CDS             complement(10305..10724)
                     /locus_tag="RHV13_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV1239972.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(10795..11550)
                     /locus_tag="RHV13_000411"
     CDS             complement(10795..11550)
                     /locus_tag="RHV13_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1239973.1"
                     /translation="MCAFLLSLVLPVQATSFTEYLPMSDSEYAQKRALKPLLTMPYDA
                     EQNWHFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSWSVPVGVLQNMAGNAQLYRAD
                     LDRNGIQDLVIWRGISGNGLAPNAFLILMTFNQQGRPCVFQSDGFYTASETGIDDLLD
                     LQRNGHTQLLDMQFDSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYTPNRKLV
                     LKPIAGRDPQTEDLAQTQRCLIKGDVLYGVNQN"
     gene            complement(11593..12063)
                     /gene="hycI"
                     /locus_tag="RHV13_000412"
     CDS             complement(11593..12063)
                     /gene="hycI"
                     /locus_tag="RHV13_000412"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HDV1239974.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEVE"
     gene            complement(12056..12466)
                     /gene="hycH"
                     /locus_tag="RHV13_000413"
     CDS             complement(12056..12466)
                     /gene="hycH"
                     /locus_tag="RHV13_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HDV1239975.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(12463..13230)
                     /gene="hycG"
                     /locus_tag="RHV13_000414"
     CDS             complement(12463..13230)
                     /gene="hycG"
                     /locus_tag="RHV13_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HDV1239976.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(13230..13772)
                     /gene="hycF"
                     /locus_tag="RHV13_000415"
     CDS             complement(13230..13772)
                     /gene="hycF"
                     /locus_tag="RHV13_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HDV1239977.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(13782..15491)
                     /gene="hycE"
                     /locus_tag="RHV13_000416"
     CDS             complement(13782..15491)
                     /gene="hycE"
                     /locus_tag="RHV13_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HDV1239978.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(15509..16432)
                     /gene="hycD"
                     /locus_tag="RHV13_000417"
     CDS             complement(15509..16432)
                     /gene="hycD"
                     /locus_tag="RHV13_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HDV1239979.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGALVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(16435..18261)
                     /gene="hycC"
                     /locus_tag="RHV13_000418"
     CDS             complement(16435..18261)
                     /gene="hycC"
                     /locus_tag="RHV13_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HDV1239980.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLSVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(18258..18869)
                     /gene="hycB"
                     /locus_tag="RHV13_000419"
     CDS             complement(18258..18869)
                     /gene="hycB"
                     /locus_tag="RHV13_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HDV1239981.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPATARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(18994..19455)
                     /gene="hycA"
                     /locus_tag="RHV13_000420"
     CDS             complement(18994..19455)
                     /gene="hycA"
                     /locus_tag="RHV13_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HDV1239982.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            19667..20017
                     /gene="hypA"
                     /locus_tag="RHV13_000421"
     CDS             19667..20017
                     /gene="hypA"
                     /locus_tag="RHV13_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HDV1239983.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            20021..20893
                     /gene="hypB"
                     /locus_tag="RHV13_000422"
     CDS             20021..20893
                     /gene="hypB"
                     /locus_tag="RHV13_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HDV1239984.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIASAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            20884..21156
                     /gene="hypC"
                     /locus_tag="RHV13_000423"
     CDS             20884..21156
                     /gene="hypC"
                     /locus_tag="RHV13_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HDV1239985.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPEVGALLYGEEK"
     gene            21156..22277
                     /gene="hypD"
                     /locus_tag="RHV13_000424"
     CDS             21156..22277
                     /gene="hypD"
                     /locus_tag="RHV13_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HDV1239986.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            22274..23284
                     /gene="hypE"
                     /locus_tag="RHV13_000425"
     CDS             22274..23284
                     /gene="hypE"
                     /locus_tag="RHV13_000425"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HDV1239987.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            23358..25436
                     /gene="flhA"
                     /locus_tag="RHV13_000426"
     CDS             23358..25436
                     /gene="flhA"
                     /locus_tag="RHV13_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HDV1239988.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFAHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(25473..25826)
                     /locus_tag="RHV13_000427"
     CDS             complement(25473..25826)
                     /locus_tag="RHV13_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HDV1239989.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(25903..26037)
                     /locus_tag="RHV13_000428"
     CDS             complement(25903..26037)
                     /locus_tag="RHV13_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1239990.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            26113..28674
                     /gene="mutS"
                     /locus_tag="RHV13_000429"
     CDS             26113..28674
                     /gene="mutS"
                     /locus_tag="RHV13_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDV1239991.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAGLQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            28780..29436
                     /gene="pphB"
                     /locus_tag="RHV13_000430"
     CDS             28780..29436
                     /gene="pphB"
                     /locus_tag="RHV13_000430"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HDV1239992.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLNVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHQLPHIIEITNDNIKYVITHADYPGNEYLFGKEIAEIELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGWLSFYKIK
                     "
     gene            complement(29487..30254)
                     /locus_tag="RHV13_000431"
     CDS             complement(29487..30254)
                     /locus_tag="RHV13_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDV1239993.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIADYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRVLAKQDLSLLVAKNE"
     gene            30450..31358
                     /locus_tag="RHV13_000432"
     CDS             30450..31358
                     /locus_tag="RHV13_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDV1239994.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            31355..32617
                     /locus_tag="RHV13_000433"
     CDS             31355..32617
                     /locus_tag="RHV13_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HDV1239995.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQARKAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSIETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            32614..33252
                     /locus_tag="RHV13_000434"
     CDS             32614..33252
                     /locus_tag="RHV13_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDV1239996.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            33257..34033
                     /locus_tag="RHV13_000435"
     CDS             33257..34033
                     /locus_tag="RHV13_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HDV1239997.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALELNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFAPHGKRILVEALSPGVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            34122..35486
                     /locus_tag="RHV13_000436"
     CDS             34122..35486
                     /locus_tag="RHV13_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HDV1239998.1"
                     /translation="MSTITLLCIALTGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(35525..35761)
                     /locus_tag="RHV13_000437"
     CDS             complement(35525..35761)
                     /locus_tag="RHV13_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1239999.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLAEGKR"
     gene            complement(35772..37199)
                     /locus_tag="RHV13_000438"
     CDS             complement(35772..37199)
                     /locus_tag="RHV13_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HDV1240000.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNTPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPVAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTRPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARK"
     gene            complement(37199..37792)
                     /locus_tag="RHV13_000439"
     CDS             complement(37199..37792)
                     /locus_tag="RHV13_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HDV1240001.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAATISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            37939..38346
                     /locus_tag="RHV13_000440"
     CDS             37939..38346
                     /locus_tag="RHV13_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDV1240002.1"
                     /translation="MALRNKAFHLLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDAADKRRRFVWLTAEGEKILAAA
                     IPIGDSVDEEFLGRLSAEEQELFVQLVRKMMNT"
     gene            complement(38466..39458)
                     /gene="rpoS"
                     /locus_tag="RHV13_000441"
     CDS             complement(38466..39458)
                     /gene="rpoS"
                     /locus_tag="RHV13_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HDV1240003.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(39521..40660)
                     /gene="nlpD"
                     /locus_tag="RHV13_000442"
     CDS             complement(39521..40660)
                     /gene="nlpD"
                     /locus_tag="RHV13_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HDV1240004.1"
                     /translation="MSAGSPKFTVRRIAALSLVTLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(40800..41426)
                     /gene="pcm"
                     /locus_tag="RHV13_000443"
     CDS             complement(40800..41426)
                     /gene="pcm"
                     /locus_tag="RHV13_000443"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HDV1240005.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEKHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(41420..42181)
                     /gene="surE"
                     /locus_tag="RHV13_000444"
     CDS             complement(41420..42181)
                     /gene="surE"
                     /locus_tag="RHV13_000444"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HDV1240006.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(42162..43211)
                     /gene="truD"
                     /locus_tag="RHV13_000445"
     CDS             complement(42162..43211)
                     /gene="truD"
                     /locus_tag="RHV13_000445"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HDV1240007.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(43208..43687)
                     /gene="ispF"
                     /locus_tag="RHV13_000446"
     CDS             complement(43208..43687)
                     /gene="ispF"
                     /locus_tag="RHV13_000446"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HDV1240008.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(43687..44397)
                     /gene="ispD"
                     /locus_tag="RHV13_000447"
     CDS             complement(43687..44397)
                     /gene="ispD"
                     /locus_tag="RHV13_000447"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HDV1240009.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(44416..44727)
                     /gene="ftsB"
                     /locus_tag="RHV13_000448"
     CDS             complement(44416..44727)
                     /gene="ftsB"
                     /locus_tag="RHV13_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HDV1240010.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(44921..45244)
                     /locus_tag="RHV13_000449"
     CDS             complement(44921..45244)
                     /locus_tag="RHV13_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HDV1240011.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLMTVGIMFGALF
                     MWLLG"
     gene            complement(45294..45899)
                     /gene="cysC"
                     /locus_tag="RHV13_000450"
     CDS             complement(45294..45899)
                     /gene="cysC"
                     /locus_tag="RHV13_000450"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HDV1240012.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(45899..47326)
                     /gene="cysN"
                     /locus_tag="RHV13_000451"
     CDS             complement(45899..47326)
                     /gene="cysN"
                     /locus_tag="RHV13_000451"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HDV1240013.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(47328..48236)
                     /gene="cysD"
                     /locus_tag="RHV13_000452"
     CDS             complement(47328..48236)
                     /gene="cysD"
                     /locus_tag="RHV13_000452"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HDV1240014.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            48488..49525
                     /gene="iap"
                     /locus_tag="RHV13_000453"
     CDS             48488..49525
                     /gene="iap"
                     /locus_tag="RHV13_000453"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HDV1240015.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   49607..49940
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=49668..49696
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacac"
     gene            complement(49941..51944)
                     /locus_tag="RHV13_000454"
                     /pseudo
     CDS             complement(49941..51944)
                     /locus_tag="RHV13_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510813.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
BASE COUNT        12274 a        14139 c        13580 g        11951 t
ORIGIN      
        1 aatctgcaat tagcaagaca tctttttaga acacgctgaa taaattgagg ttgctatgtc
       61 tattgtggtg aaaaataaca ttcattgggt gggtcaacgt gactgggaag tgcgtgattt
      121 tcacggcacg gaatataaaa cgctgcgcgg cagcagctac aacagctacc tcatccgcga
      181 agaaaaaaac gtgctgatcg acaccgtcga ccataaattc agccgcgaat ttgtgcagaa
      241 cctgcgtaat gaaatcgatc tggcggatat cgattacatc gtgattaacc atgcagaaga
      301 ggaccacgcc ggggcgctga ccgaactgat ggcacaaatt cccgatacgc cgatctactg
      361 tacagccaac gctatcgact cgataaatgg tcatcaccat catccggagt ggaattttaa
      421 tgtggtgaaa actggcgaca cgctggatat cggcaacggc aaacagctca tttttgtcga
      481 aacaccaatg ctgcactggc cggacagcat gatgacttac ctgacaggcg acgcggtgct
      541 gttcagtaac gatgctttcg gtcagcacta ctgcgacgag catctgttca acgatgaagt
      601 ggatcagacg gagctttttg agcagtgcca gcgttactac gccaatatcc ttacgccgtt
      661 cagccgcctg gtaacgccga aaattaccga gatcctgggc tttaacttac cagtcgatat
      721 gatagcgacc tctcacggcg tggtatggcg cgataacccg acgcaaattg tcgagctgta
      781 cctgaaatgg gcggctgatt atcaggaaga cagaatcacc attttctacg acaccatgtc
      841 gaataacacc cgcatgatgg ctgacgctat cgcccagggg attgcggaaa ccgacccacg
      901 cgtggcggtg aaaattttca acgtcgcccg aagcgataaa aacgaaatcc tgaccaatgt
      961 cttccgctca aaaggcgtgc tggtcggcac ttcgacgatg aataacgtga tgatgccgaa
     1021 aatcgccggg ctggtggagg agatgaccgg attacgcttt cgtaacaaac gcgccagtgc
     1081 tttcggctct cacggctgga gcggcggtgc ggtggatcgt ctttccacgc gcctgcagga
     1141 tgcgggtttc gaaatgtcgc ttagcctgaa agccaaatgg cgaccggacc aggacgctct
     1201 ggagttatgt cgtgaacacg gtcgcgaaat agcccgtcag tgggcgctcg caccgctgcc
     1261 gcagagcacg gtgaatacgg tggttgaaga agaagcctct gccaccacga cggctgacct
     1321 cggcccacgg atgcagtgca gcgtctgcca gtggatttac gatccggcaa aaggcgagcc
     1381 aatgcaggac gttgcgccag gaacgccgtg gagtgaagtc ccggataact tcctgtgccc
     1441 ggaatgctcc ctcggcaaag acgtctttga cgaactggca tcggaggcaa aatgagcaac
     1501 ggcattgtga tcattggttc gggcttcgcc gcccgccaac tggtgaaaaa tattcgcaaa
     1561 caggacgcca gtattccatt aaccctgatt gccgccgaca gcatggatga gtacaacaaa
     1621 cctgacctca gccatgttat cagtcagggg caacgtgccg acgaccttac ccgccagacg
     1681 gcaggtgaat ttgccgagca gtttaatctg cacctgtttc cgcacacctg ggtgacggat
     1741 atcgatgccg aagcccatgt ggtgaaaagc cagaataatc agtggcaata cgacaagctg
     1801 gtgctggcaa ctggtgccag tgcctttgtc ccgccagtgc ctgggcgtgt gttaatgctg
     1861 acgttaaata gtcagcaaga gtatcgcgcc tgtgaaacgc aactgcggga tgcccgacgc
     1921 gtgttgattg ttggcggtgg cttgattggg agcgaactgg cgatggattt ttgtcgggca
     1981 ggcaaagcgg tcacgctgat cgacaacgct gccagtattc tggcgtcgtt aatgccaccg
     2041 gaagtaagca gccgcttgca gcatcggttg acggagatgg gcgttcatct gctgttgaaa
     2101 tctcagttgc aggggctgga aaaaacggat tctggcattc tggcaacgct ggaccaccag
     2161 cgcagcatcg aagtggatgc ggtaattgcc gccaccggac tgcgcccgga aaccgccctg
     2221 gcacgacgcg ccgggctgac gattaatcgc ggcgtttgcg tcgatagtta tctgcaaacc
     2281 agtaatgccg atatttacgc gctgggcgat tgcgcggaaa ttaacggtca ggtattgccg
     2341 ttcctgcagc cgattcaact tagcgcgatg gtgctggcaa aaaatcttct cggcaataac
     2401 acgccgctga aactaccggc gatgctggtg aaaatcaaaa cgccggaatt accgctgcat
     2461 ctggcaggcg aaacccagcg tcaggattta cgctggcaga ttaataccga aagccaggga
     2521 atggtggcgc gcggtgttga cgatgcagac caacttcgcg cctttgtggt cagtgaggat
     2581 cggatgaaag aggcatttgg attgttgaaa acattgccgg tgtaggcggg ctactgtgcc
     2641 caaaatgtcg gatgcgacgc tgacgcgcct tatccgacct acggggagcg catgtgttgg
     2701 tcacataatg cgttcatgtc gcatccgcct actgtgccca aaatgtcgga tgcgacgctg
     2761 gcgcgtctta tccgacctac ggggagcgca tgtgttggtc acataatgcg ttcatgtcac
     2821 atacagctac tgtgcccaaa atgtcggatg cgacgctggc gcgtcttatc cgacctacgg
     2881 ggagcgcata tgtaagccgg ataaggcatt tacgccgcat ccggcaaggg tgtccaaatg
     2941 caacacgctt tatccgttct ggacttcacc cgctaaccaa cgcgccgcag caataacccc
     3001 ctgccccaga gacaaaccgc catcacccgc cggtaaactc tgcgggaaga gcaatgtgaa
     3061 atcagcgaga taatgcgcca gacgtgcacg cagcaaacgg ttatgaataa ccccgccgct
     3121 aaataccagc gtagtgatac cgtgcatcgt ggcctgctca cgcatcaacg cggcaaaacc
     3181 ctgcgccagc gcatcatgaa acgcccacgc gcgttgatta accggagcct gccagttcag
     3241 ccactgctgc cagaaagtgg cgagatccag ttgattgtcc accagcggca tcgtcaccgg
     3301 atgcgtcact ccgtcgcatg aggctgcgag cgcctccaga gcacaagccg cttcaccttc
     3361 ataacttaac gtggctggcg cacagcccag tgccgccgcc acagcatcga acaaacgccc
     3421 acacgatgac gccagcggcg cgttaattcc acgctcaatg gcccgcgcca gcacgctcca
     3481 gttttgctgt tgcacacttg ccgtttcagg gtaattctgc cactccggca caaagcgcag
     3541 gcactgcgcc agcaggttac gccacggctg cttcgccgcc aaatcgccac ccggaagcgc
     3601 cactgcaggc aagccgccca ggtgctcaca ttcgcgatag ttcacccgca ggcactcgcc
     3661 gccccacaaa gcgccgttct cccccatacc gataccgtcg agcgtcaaag caatgacatc
     3721 accgccatcc agcggccact gatgctctgc cagacacgcc gctgcatggg catgatgatg
     3781 cagtaccgtt tgcgtcggca gattcatttc gctcgcccac tggctggaga catagcccgg
     3841 atgcacgtca tgcacaacgt attgcggggt gaaatcgtag atgttttgca tcaggcgtaa
     3901 cgcttcgcgc cactgcatct ggatgccatc gtcacttaaa tcgcccagat gctggctcaa
     3961 caccgcttgt tcaccgcgca ccaggcagaa ggtatttttc agatccgcgc cgagacacag
     4021 cacaggcgga acatttttaa agcccggagg caaagccagc gcatccggca catacccccg
     4081 cgaacggcgc atcatttcgc cgctttcgcg caccactgaa tcatccatcc gctgcacgat
     4141 gtcgcggtta tgtattaaga atccgtcggc aatgccctgc aaatcctcca gcgcctgttc
     4201 gttgctgata gccggtggtt taccgctcag gttgccagag gtcatcacca gcgggcattg
     4261 cagttcctgt aacagcaaat gctgaagcgg attcgcaggc aacattaccc cgacttcgtt
     4321 aaggccaggg gcgatatcat cacaaatctc aggtacgtat tttttatcca ccagcacaat
     4381 cggcgcggcg ggcgtggtga gcaactgacg ggcagcgtct ggtaaacctt cagccactgg
     4441 caacatgact gcaagcggtt tcgccgggcg atgtttgcgc gcccgaagtg tcgccaccgc
     4501 gttactgtta cgtgcatcgc aggcaagatg aaatccgcca atccctttga tggcgacaat
     4561 gttgcccatt tttaactgtg cgatagctgc ctgtaatgcc gcctcttgtt ctgcatgttc
     4621 accatgactt acccattcaa gatgcggacc acactccggg caggccaccg gctgggcgtg
     4681 gaagcgacga tcgagcgggt cgcggtactc tttatcacag gctggacaca gcggaaacgc
     4741 cgccatcacg gtaaacgggc gatcgtaagg catggcgcga ataatggtga agcgcgggcc
     4801 gcagtgggta cagttgataa atggataacg ataacgccgt tcgcctgggg tattcatttc
     4861 ggcaaggcaa gcagggcaag tggcggcatc gggaacaatt tgcgtattca tggcgccgcc
     4921 cgcgctctga cggatagtga actcagtggg cagttgtgac cagataaacg gttcacgctc
     4981 gacgctatca atacgcgcca gcggcgggca gtgctgatgc aattgaacaa gaaacgtttc
     5041 cgggtcttcc agcagccgga cttctacgcc atcgccgtca ttacagacat cgccgcgaag
     5101 atttaattgc tgtgccagct gccagacaaa cggacgaaaa ccgacgccct gcactttgcc
     5161 acgaatacgc agttggacac cgcaagatgt gttttttgcc attgagttat tcccgccatc
     5221 atgaattgcg taacccgtcc agccggacac gacagcgtcg catccggcag tcacaggttg
     5281 gcgataccgc ccgctccgta ttctacgaat atttccggga attcctttga tgccagaaca
     5341 gttctgtaag atttttagaa catcagcgcc gtacggcggc gtttttctgc gctcagttgt
     5401 tcaagtttat tacgatcgac acaaatcagc gcatgagtcg ggcaagccgc catacacgcc
     5461 ggaccgtctt cacgatggtt gcacaggtcg catttattgg cttcggcttt gtcagcccgt
     5521 acattcagcc ctgcgccgct gttgcgaatc accggacgta ccaccacttc catcgcgcca
     5581 tacgggcaag ccacaacgca ggttttgcaa ccaatgcaac gttcctgcat cacatgaaca
     5641 aaccctttat cacggctgat agcaccattc gggcagacgt tagcgcacgg tgcatcttca
     5701 cactgacggc aaactgtcgc cgtggaaatg ttcacacctt taatgacatg gatacgcggt
     5761 aaaaatgttt ccggggtcag cgatgcacag tcctgattct cctgatgaga aaccacgcac
     5821 gctacttcac aggtacggca accaatacat ttactcgcgt cagcaatgat gaaacggttc
     5881 atcaaattct ccagcaatga cagttaatgc gccgatacat tcacaaatca tgccagtttt
     5941 taatttactg ttatttaagg aaattaaatt ctgtgatgca ggaaaaacga tgtcatcgac
     6001 actagtgacg atgacatgtg atgacaatgt ttatcgcgaa agagcaatga gtgagtcgcg
     6061 gcggatcagt tttccgctga aggttttcgg cggtgagaaa tccccgccat cgagcataaa
     6121 aatcagccgt ccaataattt cctgaatcat ctcagtcacc ggaattttta cgctggagag
     6181 cgccggaacg atataggggg caattgcgat atcatcgaat ccgataactg acacctgctc
     6241 tggcaccgct acgccgcgct cgtgtaacac tttcatcgca cctatcgcca tatcgtcgtt
     6301 actggcaact aacgcgctaa atttagcccc acgttcgagc aacgtttcta ccccttcggc
     6361 cccgctggcg ggcgtccatt taccgttagc gataagtttt tcattgagcg caatactatg
     6421 ctgcgccagc gcgtctttat acccggcaag acgttcaatg ctggtgggag aatccatcga
     6481 gccggtaagg aaagcaatct cctgatgccc ggcgtttatc aactctgcca cggcgttaaa
     6541 gctggtctgt ttatgatcgc accagacgct atggctgcta tttttgcgca ggcggcgatt
     6601 aagcaccatt atcggctgac tgtgcgcgtc aatgatgtca tcgatctcat ccacacttaa
     6661 aaaacgcggg taaatcataa tggcgtcgca gcgcaaatcc agcagatact gaatcgcctg
     6721 gcgctcttct tcggcgctgt gtttaccatc tgccaatagc aactgccgcc ctttctcttc
     6781 cgccattcgc gcggcatgaa acagtaattc actaaaataa atgccgtggt aaagcgtgtt
     6841 agtcactacc agccccagcg tctgggtact tttcgccgac agattgcggg ccagcaaatt
     6901 tgggcggtaa ccgctctctt ctaccgcctg aaacacgcga tctttagtct cctggctgac
     6961 gtagccatta cctgaaagca cgcgggaaac ggtcgctttt gaaaccccgg cgcgcttcgc
     7021 cacttccagc atcgtcgtca tcattttcat ccctttacac gcaatcaacg cagtgtactg
     7081 caccgtttgc cgattgtcct tgcgcaatcg gcgggaaaaa tattcaggtg accggtttca
     7141 caaatataaa aaatgaacaa ttcactctct tgcttattca gtgacaacta ttcatgattt
     7201 tgtgaaaccg gtttcttaat tccgtttcag catcggcatt tttccgtcac gtcgactgat
     7261 aacaactaca tctaccctac tgataacagg ataaaatccg atggccaaaa attatgcggc
     7321 gctggcacgc tcggtgatag cggcactggg cggcgttgat aacatctcgg cagtcaccca
     7381 ctgtatgacg cgtttgcgct ttgttatcaa agatgatgcg cttatcgaca gcccgacgtt
     7441 aaaaaccatc cccggcgtgc tcggcgtggt acgtagtgac aaccagtgtc aggtgattat
     7501 cggcaatacc gtttcacaag cctttcagga agtcgtcagc ctgctgccgg gagatatgca
     7561 gcccgcacag cccgtgggta aacccaaact cacgctgcgt cgcattggtg cggggatcct
     7621 cgatgcgctg atcggcacca tgtcaccgct gatcccggcg attatcggcg gatcgatggt
     7681 caaactgctg gcgatgctcc tcgagatgag cggcgtgctg acaaaaggat cgccgacctt
     7741 aaccattctg aatgtgattg gtgacggtgc tttcttcttc ctgccgctaa tggtcgcggc
     7801 ttcggccgcc atcaaattta aaaccaatat gtcgctggcg attgccatcg cgggtgtgct
     7861 ggtacatccg agttttattg aattaatggc gaaagcggcc cagggtgaac atgtcgaatt
     7921 tgccctgatc ccggtcaccg cggtgaaata cacctacacg gtgatcccgg cgctggtgat
     7981 gacctggtgc ctgtcatata tcgaacgctg ggtggatcgc attacaccgg cggtgaccaa
     8041 aaacttcctc aagccaatgc tgattgtgtt gattgccgca ccgctggcaa tcctgctgat
     8101 tggcccgatt ggtatctgga tcggtagcgc catttcggcg ctggtttaca ccattcatgg
     8161 ttatctgggc tggctttcag tcgccattat gggcgcgctg tggcctctgc tggtaatgac
     8221 cgggatgcac cgcgtcttta cgccaaccat cattcagacc attgccgaaa ccggcaaaga
     8281 agggatggtc atgccgtcag agatcggcgc taacctgtcg ctgggcggtt catcactggc
     8341 ggtggcgtgg aaaacgaaaa acccggaact gcgccagacg gcgctggctg cggcggcatc
     8401 agccattatg gcggggattt ccgaaccggc gttatacggc gtggcgatcc gcctgaaacg
     8461 tccgcttatc gccagtctta tcagcggttt tatttgcggc gcggttgccg gtatggcggg
     8521 gcttgccagc cactcaatgg cagcgccggg gttatttacc agcgtgcaat tcttcgatcc
     8581 ggcgaatcca atgagcatcg tctgggtgtt cgcggtcatg gcgctggcgg tcgtgctgtc
     8641 gtttatcctc acactgttgc tcggctttga ggatattcct gttgaggaag cggctgccga
     8701 ggcgcgaaag catcagagcg cacaaccgac cgtcgccaaa gaagtaagtc ttaattgagg
     8761 atgaaaatgt cagtatttcc agaaggtttt ttatggggcg gcgcgcttgc cgccaaccag
     8821 tctgaaggtg cgttccgtga aggcggcaaa ggactgacaa cggtcgatat gatcccacac
     8881 ggcgagcatc gaatggcggt gaaactgggg ctggaaaaac gttttcagtt gcgcgatgac
     8941 gagttttatc ccagccatga ggcgacggat ttttatcatc gttataaaga agatatcgcc
     9001 ctgatggcag agatgggatt caaagtgttc cgtacctcaa ttgcctggag ccgcctcttc
     9061 ccgcagggcg atgaactgac gcccaaccag cagggcattg ctttttaccg ttctgtcttt
     9121 aaagagtgta aaaagtacgg tatcgaaccg ctggtcacgt tgtgccactt cgatgtgccg
     9181 atgcatctgg tcaccgaata tggctcctgg cgcaaccgca agctggtgga gtttttcagc
     9241 cgctacgccc ggacctgctt tgaagcattt gatagcctgg tgaaatactg gctaaccttc
     9301 aatgaaatca acattatgtt gcatagcccg ttctccggcg cgggtctggt gtttgaagaa
     9361 ggtgaaaatc aggatcaggt gaaatatcag gccgcgcatc accagctggt tgccagtgcg
     9421 ctagccacca aaatcgccca tgaggttaac ccgcaaaatc aggtgggctg tatgctggcg
     9481 ggcggtaact tctaccctta cagttgcaag ccggaagatg tctgggcggc gctggagaaa
     9541 gatcgggaaa acctgttttt tatcgatgtg caggcgcggg gcacgtatcc ggcttactct
     9601 gcccgcgtat tccgcgaaaa aggggtaacc atcgacaaag caccgggcga tgatgaaatc
     9661 ctgaaaaaca ccgtcgattt tgtctctttc agctattacg cctcgcgctg cgcctcggcg
     9721 gagatgaacg ccaacaacag cagtgcggcg aacgtggtga aatcgctgcg taatccgtat
     9781 ctacaggtga gcgactgggg ctggggaatt gatccactcg gtctgcgtat caccatgaat
     9841 atgatgtacg atcgttatca gaaaccgctg tttctggtgg aaaacggcct cggcgcaaaa
     9901 gatgaacttg ctgccaatgg cgagattaac gatgactatc gcatcagcta tttacgcgaa
     9961 catatccgcg caatgggcga agcgattgca gatggcattc cgctgatggg ctacaccacc
    10021 tggggctgta ttgatttagt ttccgcctct acgggtgaaa tgagcaaacg ctacggtttt
    10081 gtctacgtag accgtgacga cgcaggcaac ggcacgctga cgcgcacgcg taagaaatcg
    10141 ttctggtggt ataaaaaagt aattgccagt aatggggaag atttagagta agtaaccgtg
    10201 ccggatgcgg cgtgaacgcc ttatccggcc tacatagttc gtgcgacatg taggcccgat
    10261 aagcgcagcg catcgggcaa tctggggttt ggcatcagtc aaaatcaaag aaaccattcc
    10321 ggcttaacgc caaatcgggc tgaaagtgcc ttaatgtggg atattgtcag ggatcgttgt
    10381 ccagacaaaa tctgactgac cagggattta gagccgattt cgttcttcaa atccgcatag
    10441 gtcagattat gttgatcaat caacgtgcga agtaacgcca cgccaaccgg cattgccgca
    10501 acggctttgt cgaactctgc aaacttttcg ttgttgtttt catactctgc aatgcggctc
    10561 gccagaaagt cgataagtgg attggtgtca tcttcttcaa tcaaatattc gaccagttcc
    10621 agtgcttcac gataatcatc ttctgagtca ctgcctccca gaaaggggac tgcattgacc
    10681 agttctcttg ttgctttaac agcgcgtgca gcattagcag tcattctttg ttctctctgt
    10741 agtaacgtga cgtctgtctg cacgtaatcc ccctcatagc cactctgcaa aaagtcagtt
    10801 ctggttcacc ccataaagca catccccctt tatcaagcaa cgctgcgtct gcgccagatc
    10861 ttccgtttgc gggtcgcgcc cggcgatggg tttgaggacg agtttacggt tgggggtata
    10921 agtaaaacgc gtgagcgcgg gatagcttaa cttgccaaac cagccgtgaa cacgctgcca
    10981 tttggcgtct tttacctgat acagactggt tatccagtaa ccggaatcaa actgcatatc
    11041 cagcaattgc gtgtggccat tcctttgtaa atccagcaaa tcatcaatgc cagtttcact
    11101 tgcggtataa aaaccatcgg actgaaaaac acacgggcga ccttgctgat taaaggtcat
    11161 cagaatcaaa aacgcgttag gtgccagacc atttcctgaa ataccccgcc agataaccag
    11221 atcctgaatg ccgttacgat caagatcggc acggtaaagt tgcgcattac ccgccatatt
    11281 ttgcagcacc cccacaggca cagaccagga tttaccggcg cgatctttac cgttcaattg
    11341 ccattcacta tcatcatttg gcatcttttc aagcgtcacg ccagcaacgc cgacttttct
    11401 aaaatgccag ttttgctcgg catcataagg catggtcagg agaggtttca gcgcccgctt
    11461 ttgcgcgtat tcgctgtcac tcatcggcag atattcggta aagctggtgg cctgtacagg
    11521 cagcactaag ctcaataaaa aagcgcacat ccctgttctg atgttcaatt ccctgaattc
    11581 cttaatgaaa aactactcta cttccaccgc taactgcgca aatccaccat ttccttccca
    11641 gccttccagt cgttgataaa cggtttcaac cgcatcttta atcggctggg tcatcgggta
    11701 gtaaaagccg acgatatccg gctggatgcc gaggaaaatc acttcgccaa tatcttcttt
    11761 caactgatcg ataaggtagt tcaacggcat gttatgggta gtcatcataa acatctcggc
    11821 gatatcatcc gggtcgatga tgcggatttc gccggggttt agccccatat ccgtggcgtc
    11881 gacaatcagc agccgtgtcg ggcgcagttc acggatggcg acgatgtcgt tttccggtgc
    11941 gctaccgccg tcaatcacca cccagttacc tttcggcgcg gcggcgcact tttccgccag
    12001 cagcggacct gcgccatcat cacccatcat gctattgcca acacagagta aaacgtcagt
    12061 cacgcagtct cctcaccatc aaatagatgg cgttttcctg atgaatatca tgcagcatac
    12121 ttaacagcat tgtactccat tcctgctgct gcgaagtttg cgctgcccgg gctttatcga
    12181 atgcattggc cagcatcggc acatggttaa tgtcgatgac gatctcaccg tacttcggta
    12241 cgccttccat tttgcggcgg gcttcgctgc ccgcctccag agtggcaatc catgccagat
    12301 attcatccca cgggcaggtg agcgccgctt ccaggcaatc gataacccca aggtggtgac
    12361 caatcgccag gctgtaatag accacctgct gcgcctcggc gggcgtggca tcgttctcat
    12421 caataaattt acggctcagt tgactgaaca ccaccttttc actcatcgga tacgcgcctc
    12481 ttcaacaaca tgattcagat ggctgacaat ctcgttcaga cgcggatcgt tttccgcttc
    12541 cagccagcgt gcaacctgtt cttcgccctg ccctaactgt gtaaggtaat catcggcaat
    12601 ctgacgaccg taacgataac ccgccagtcg acgtgcttcg cgatccactt tcacacgcag
    12661 cggctgcacc atatcgccat gcaggatctc cgccggttgt tcatcctgct cacccggccc
    12721 acgggcgtga attttctgct ccagcaggcc gagcgccatt gcaaagccgt acagcgtggc
    12781 ggcaggcgtt ggcgggcagc cagggatata aacatccacc gggacgattt tatccgtacc
    12841 gccccacacg cagtagagat cgtggaagat cccgccgctg ttaccgcagg caccgtagga
    12901 gatacaaatt ttcgggtccg gcgcggactg ccacgcacgc agcgcagggg atcgcattgc
    12961 acgggtgacc gcgccggtaa acagtaaaat atccgcatga cgcggtgaag gaacgacttt
    13021 aatgccgaag cgttctgcat caaacagcgg cgaaagcgtg gcgaaaattt cgatttcgca
    13081 gccgttgcag ccgccgcagt ccacgcggta aacgtaggca gagcgtttga tttttttcag
    13141 taacgacgcc ttcatactgg cgatggattc atccaccgtc atcgggaccg gaatgccgtt
    13201 ggcgtcacgg gggcctaata aattgctcat cagatggcct ctttcatatg gcgagtcagt
    13261 tcaatacggt cggacggcac caggcatttc tggcgcttac attccgggca agtctcaaag
    13321 ctttcgcggt ggttttccgc gcggctgtcg ccattgtgct taagcagcgc aatggcgtag
    13381 tcgatctctt tctggacggc gaaaggacga ttgcagacgc ggcagttgca cagcgcgaag
    13441 cgggactgtt gcaggaagtc ttctttcttc cacaccgcca gttcgtactc ttgcgacagt
    13501 ttgatcgccg ccgtcgggca aacttcttcg cagcgtccac agaagatgca acgcccgaga
    13561 ttaaactccc aggcaagctc tcctgtggcg aggtcggttt caaccgttaa ggcgtttgac
    13621 gggcaggcat tgacgcaggc cgcgcagccg atgcactgct gcgggttctg ctctggctta
    13681 ccacggaagt ttttatcaac cgcaatcggc tccagcggat aagacgaggt cgccgtgccg
    13741 gttttgataa cttttttgat aaaggtaaac atggcgattc cttatttcag cggcgagttt
    13801 ttacgctcaa tgctgtaacg ctcgagttct ttgtacggca ccactttgct cttcttctta
    13861 cgcacatcga ccacggtcat gcggtcggta caggagtagc aagggtcgag gctaccgata
    13921 atcagcggcg catcggaaac ggtattgcca cgcagcatgt agcgcagggt cggccagttc
    13981 gcgtaggtcg cggcacggca gcgccagcgg tacagcttct ggttgtcgcc ggtcatgctc
    14041 cagtggatat cgtcgccgcg cggcgcttcg gcaaagccca gcgcaaagcg gtgcggaatg
    14101 taggtaaagc cttccaccat cagtgggcca cccggcaggt tatccagacc gtagtcgatc
    14161 atgttcagcg cggtatagac ttcgttgata cgcactttca gacgggaaat aacgtcgcag
    14221 ccctgctcgc tgtggacttc cattggcagc aggccatagc cgacaaatgg gtgatctgcg
    14281 cgggtatcgc gggcgtgacc gctggcgcgg accatcgggc caacgttact gaagtcgcga
    14341 gcgatttccg ggtccagacg accaatgccg acagtgcgct gttccatgtt cggcgtactc
    14401 agcagcacat ccaccagctc ctgcacttca cgacgcatct gttgcgccag ctggcgggtc
    14461 tggatcatgt catctttcag cagatcgcga cgaatcccgc cgatcaggtt caggccgtag
    14521 gttttacgcg caccagtaag gatctccgcc attttcatgg aggtttcacg cacgcggaag
    14581 aactgcataa agccggagtc aaagccggta aagtggcagg caagaccgag gttgagcaga
    14641 tgcgagtgca ggcgttccac ctccagcaga atggcgcgga tcatctgcgc acgttctggc
    14701 acctgaatac ccatcgcgtt ttccaccgac gtggtgtagg cggtgctgtg agcaaagccg
    14761 cagatcccgc acacacggtc agagaggaag gtcacttcgt tataacccat gcgggtttcc
    14821 gccagttttt ccatgccgcg atggacgtag aacagacggt agtcggcgtc gataatgttt
    14881 tcgccatcga cgaacagacg gaagtgaccc ggttcgtcgg aagtgacgtg caacggacca
    14941 atcggcacga cgttgttttt cttgtcgccc agttcgttga tgaactcgta ggtttcagca
    15001 tcggtggtcg gtgccggacg ctgacgataa tccatgctgt ctttacgcag cggataaagt
    15061 tcatccggcc agtcatccgg cagcaccaga cggcgttcat ccggcagacc aaccggaatc
    15121 aaaccgtaca tatcgcgcac ttcgcgctcg ccccacaccg ccgccggaac gcgcggcgtc
    15181 acggacggat actccggttt gttggcatca acttcgacgc gaaccgttac ccagcactta
    15241 gtccccttct ccatcgacag cacgtaataa acggcgtaat gaccattcag tttgcgttcg
    15301 tcgttaccaa aaagcaccga cagccagccc ccctgcttgt agtagagaaa ctccactact
    15361 tccggcagat agttcacctt tacggtgaca gtcagctgat ctttggtctg ccaggcgtgg
    15421 tccagcacga cgcccggaaa tgcctcattc agcgcggcga gataatgttg acctaatttt
    15481 tcttcagaca tgctcaaact ctctttaatc acgccgccag caaggagacg aacgctaaaa
    15541 atgcaaagcc aaacccagcc caggtaatgc gcggagtaat atcaagacgc agacgggcca
    15601 tgctgttttc gaacagcgcg ataaccaggg cgccgaccac cagtttgacg atggcaatca
    15661 ccagcgccag cagcagccca ccgacggtga aggtttccat ttgtccccac ggaataaaca
    15721 ccccgacgaa catctgcagc accaccagct gtttcaggct gataccccat ttcatgacgc
    15781 caaagccgct gccgctgtat tcagagagcg gaccttcctg cagctcctgc tcggcttccg
    15841 ccaggtcgaa cggcagtttg cccatttcga taaaggtggc gaacgcacag gcacaaagcg
    15901 ccagtaccag cgggatgctc tggctcagcg gccagtgata aacggtgtcg gtgatgttgc
    15961 tgatgttggt ggaaccggca acctgtgcgg caacccacag accaagcagc agcatcggtt
    16021 cgaccagcac gccaagcatc gcttcacggc tcgcgccgat agcggtaaac gggctaccgg
    16081 tatccagacc agaaatggca aagaagaaac gcgcgatggc aaagagatac agtaaggtga
    16141 tcaaatcacc cagttgcggc agcggagaac cgacggtcac caccggcagc gcagtagcga
    16201 tagtcagcat gacgcccacc atcacatacg gcgtcaggcg gaacacccag ccggaggcat
    16261 ccgggccaac gctctggcgg cccagcagtt tgataatgtc gcgatactcc tgcaacacgc
    16321 ccggcccgcg acggttatgc aagcgggcgc gcgccacgcg ggttataccg gagagcagcg
    16381 gcgcaacggc aaataacacc agcgcctgaa ttaacggata taaaacactc attctcaggc
    16441 tcctcgtgaa acaataatca ccaccagtac cgccagttca accagcgcca tccggcggaa
    16501 cagcaacgca ctcccctcgc actgccagcc cggcaccagc gacaccggat tcagccattt
    16561 gcgcagtttc agcaccggcg caaacgcctg tttcaccggc atggcaaaac cgtgagcggt
    16621 aatcaccatt gatttttcgt ggtcgtaacc gcacacccag gccgcaccgc gggaacgcga
    16681 tggcaaacga tcgcctttgc aaatcgccat aatgatgaat ggcagcagcg ggcaggcaat
    16741 cagcagcaac gtgatcattg gttgagaaac ggtggtgtta gcaggctcca gaggcagagg
    16801 tacagcagca gagagcatcg gcagcagcca cggcgcagca acaccgccaa ttacgcagca
    16861 aatcgccagt gccactacgc ttacgctcat caggagcggt gcacaggtgg cgttttcggc
    16921 ttctttggta cgcggcgcgc cgaggaacgt gacgccatag actttcgcca tacacatcac
    16981 cgccagcgca ccggtaattg ccagacccac agcgagcagc ggccccagca gacgggcaac
    17041 aaacgcgcca ctattgctca gtttgaagaa ggattgatag ataacccatt ccccggcaaa
    17101 accgttcagc ggcggcagtg cagccattgc catcagcccg actaacatag cgatggagat
    17161 aaccggcatt ttcttgccaa taccaccgag tttttcgata tcgcgatgac cggtacggaa
    17221 ccagacgctc cccgccccga ggaacagtac gcttttgaac aggctatggt taagcagatg
    17281 gtacagacca ccgaccaggc caagagcaat cagcgccggt tgttcgagcg cgatacccgt
    17341 tacgccagcg cccagcccca gcaggataat gccgatattt tccagggtgt ggtaagccag
    17401 tagacgctgg atgttatgct ccatcaacgc atacagaccg ccgacaaacg cggtgatcat
    17461 accgagcacc agcagcgcga tcccccacca cagcggtgca ttaccgccca gcagtgacag
    17521 ggttaaaatg cccagcaggc caattttcat gactaccgta gaaaacaacg cggcagctgg
    17581 cgcagaggcg ttcgcatgtg cctgcggcac ccagccgtgc agcggaataa tcccggccag
    17641 caggccaaag ccaatcacac tgagcagcca gatatcggaa ccgagcggca gctgttgcat
    17701 acgcatatcc agcaggcgca gatccagcgt gccgtaacgc tgccacagca gccagcaagc
    17761 aatcgccagc agcagagtgc caagacgccc cagcgcaaac cacagtttgc cctctttgct
    17821 gttgctggtg aggaacaccg cacacaaggc catgacttcg gccattacca cgaacatgcc
    17881 gaggttgctg gcaatgacgg cgcagacggc ggcagccatc aacatattga tctgcaagcc
    17941 gttgcacttc acctgcgcgt ggcgatgcca gtcaatgttg tagaggctga caaacagacc
    18001 gcacagaccg agcgtaatca gccaaatcgc gttaagcgga gagatttgca catcgtagct
    18061 taccagcgac agcgcaccgc tcacgccaac cgcgccagtc agtacagtga agcccgcggc
    18121 tgccgtatac agactaccaa ccgcgccgcc aattccggct atccagccgc ttagcgcctt
    18181 ttgaaaagaa aagagaaatg ccagaacagc ggcggcgaca aaccacgcca cgccgctatt
    18241 gatcagggaa attgcgctca tttagcctct ccactttgag cctgctgaaa caaggtgaga
    18301 tcgccaaagt ccgtgttaaa ggtcagctca cgcttacgtt tgctgacgcg ggcgatatcg
    18361 gtgttatcca ccagatgcag ggctttagtc gggcacatcc gcacgcaggc cggaccttgt
    18421 tcatcaaagc tacaaaggtc acatttgacg gcgatcgcgc gaatacctgg cacccagtca
    18481 agcaatgtgc tgacacgcgc cgtagcaggc ggtgccggtg gcgctttcgg ggtattggcg
    18541 tttgccggaa tatccagcgg acggctgccg gaaaattcaa ttgcgccaaa cgggcaggcg
    18601 atgccgcaca gtttgcagct tacgcacagg ctttcattca actgcacggc cccatcaacg
    18661 cgggtgatgg cgttaaccgg gcagaccacc gcgcagggtg catcttcaca gtggtgacag
    18721 agctgcggcg cagattcttt ttcatttagc atcactctca ggcgcggcat tgattgcagg
    18781 ccgtgctggc gatgcgtctc tgaacaggcg gcctcacaag tgtggcagcc gatacagagc
    18841 gtggagtcag caattacaaa acgattcacc aggcattcct caggtgattg tcatttttga
    18901 cgaaaacatg ccgatgaaat gtcattttcg acactcatcg acacgcccat ccccaaacag
    18961 gcataacgcc tgcaaaacgg gcaaagcctc agctcatgct gccgggcttt gtccctttac
    19021 cagttggctt aaattcaccg gcacattgtt ttcaatagcg gtgtataagc tgtcgtaaac
    19081 gccagcgatt ttttcgagat agtgttccag aacctgttcg cgatcgcgga tgttgacctt
    19141 gccatcaatc atgccgtcaa tgctcagttg cgcctgcgca atccgctgtt tgtcttcgcc
    19201 atcttttgtt tcgacgtaat actcgatggt gtggctgtta aagccatccg ccagggtgac
    19261 gtaaatacga aaatgttcaa aaaggacgaa acagagttct ttgtccggcg cgcagctcat
    19321 ggcgtgatgc aggcgcgctt tcgataacgt gatcccctga accagcgaat tacagtagat
    19381 gtgccactgg tcctgtaggc gacgatgccg ctgtgcgatg taatcggctt tctcgcttat
    19441 ttcccaaata gtcattgtca ggttacccgt ttaacagaga tgccagcttt aagcattttt
    19501 tgtgccaact tttaatttat tgttattaaa gcgattttta agattttggt gaatttcgtc
    19561 gccgtgtcga cgtgtcattt cgacatcatc gacattattc actgcaggga taatcaacac
    19621 tggcacaatt attgcttgta gctggcaata gttaatggga ggcgatatgc acgaaataac
    19681 cctctgccaa cgggcactgg aattgatcga acagcaggcc gcaaaacacg gcgcaaaacg
    19741 cgtaactggg gtctggctca aaattggcgc attttcttgt gtcgaaacca gctctcttgc
    19801 cttttgtttt gatctggttt gccgcggcag cgtggcggaa ggttgtaaac tgcacctcga
    19861 agaacaagag gccgaatgct ggtgtgaaac atgccaacag tatgtgacgc tactgaccca
    19921 gcgcgtccgc cgctgtccac agtgtcatgg tgacatgcta cagattgtgg cagacgacgg
    19981 tttacagatt cggcggatag aaatagacca ggagtgagcg atgtgtacaa catgcggttg
    20041 cggtgaaggc aacctgtata tcgagggtga tgaacataac cctcattccg cgtttcgtag
    20101 cgcgccattt gccccggcgg cacgcccgaa gatgaaaatc accggcatta aagcgcctga
    20161 atttaccccc agccagactg aagaaggcga cctgcattac ggtcatggcg aagcgggcac
    20221 tcacgcaccg ggcatgagcc agcgtcggat gctggaagtc gaaattgacg tgctggacaa
    20281 aaataaccgt ctggctgaac gcaaccgcgc gcgctttgct gcccgcaagc aactggtgct
    20341 caacctggtt tccagcccag gttccggtaa aaccaccctg ctgacggaaa ccttaatgcg
    20401 cctgaaagac agcgttccgt gcgcggttat tgaaggtgac cagcaaaccg tgaacgatgc
    20461 cgcacgcatt cgcgctaccg gcacaccagc gattcaggtg aacaccggta aaggctgcca
    20521 tcttgacgca cagatgattg ccgacgccgc accgcgtctg ccactggacg ataacggtat
    20581 tctgtttatc gaaaacgttg gcaacctcgt atgcccggcc agcttcgatc tcggtgaaaa
    20641 acacaaagtg gcggtgcttt ccgttaccga aggtgaagac aaaccgctga aatatccgca
    20701 tatgtttgcc gccgcctcgc tgatgctgct caacaaagtt gacctgttgc cgtatcttaa
    20761 ctttgacgtt gagaagtgca tcgccagcgc ccgcgaagtc aatccagaaa ttgaaatcat
    20821 ccttatttcc gccaccagcg gcgaagggat ggaccagtgg ctgaactggc tggagacaca
    20881 gcgatgtgca taggcgttcc cggccagatc cgcaccattg acggcaacca ggcgaaagtc
    20941 gacgtctgcg gcattcagcg cgatgtcgat ttaacgttag tcggcagctg cgatgaaaac
    21001 ggtcagccgc gcgtgggcca gtgggtactg gtacacgttg gctttgccat gagcgtaatt
    21061 aatgaagccg aagcacgcga cactctcgac gccttacaaa acatgtttga cgttgagccg
    21121 gaagtcggcg cgctgttgta tggcgaggaa aaataatgcg ttttgttgat gaatatcgcg
    21181 cgccggaaca ggtgatgcag ttaattgagc atctgcgcga acgtgcttca catctctctt
    21241 acacagccga acgccctctg cggattatgg aagtgtgcgg tggtcatacc cacgccattt
    21301 ttaaattcgg cctcgaccag ttactgcctg aaaacgttga gtttatccac ggtccgggtt
    21361 gcccggtgtg cgtactgccg atgggcagaa tcgacacctg cgtggagatt gccagccatc
    21421 cggaagtcat cttctgtacc tttggcgacg ccatgcgcgt gccggggaaa cagggatcgc
    21481 tgttgcaggc aaaagcacgc ggtgccgatg tgcgcatcgt ttactcgccg atggatgcgt
    21541 tgaaactggc gcaggagaat ccaacccgca aagtggtgtt cttcggctta ggttttgaaa
    21601 ccaccatgcc gaccaccgct atcactctgc aacaggcgaa agcgcgtgat gtgcagaatt
    21661 tttacttctt ctgccagcac attacgctta tcccgacgct tcgcagtttg ctggaacagc
    21721 cggataacgg tatcgacgcg ttcctcgcgc cgggccacgt tagtatggtt atcggcactg
    21781 atgcctataa ttttatcgcc agcgattttc atcgtccgct ggtggtcgct ggtttcgaac
    21841 cccttgatct actgcaaggc gtggtcatgc tggtggagca gaaaatagcg gcccacagca
    21901 aggtagagaa tcagtatcgt cgggtggtac cggatgccgg taacctgctg gcgcaacagg
    21961 cgattgccga tgtgttctgt gtcaacggcg acagcgaatg gcgcggctta ggcgtgattg
    22021 aatcttctgg cgtgcacctg acgccggatt atcaacgatt cgatgccgaa gcacatttcc
    22081 gcccggcacc gcagcaggtc tgcgatgacc cgcgcgcgcg ttgtggcgaa gtcttgacgg
    22141 gcaaatgtaa gccgcatcaa tgcccgctgt ttggtaacac ctgtaatcct caaaccgcgt
    22201 ttggtgcgct gatggtttcc tccgaaggag cgtgcgccgc gtggtatcag tatcgtcagc
    22261 aggagagtga agcgtgaata atatccaact cgcccacggc agcggaggcc aggcgatgca
    22321 gcaattaatc aacagcctgt ttatggaagc ctttgccaac ccgtggctgg cagagcagga
    22381 agatcaggca cgtcttgatc tggcgcaact ggtagcggaa ggcgaccgtc tggcgttctc
    22441 caccgacagt tacgttattg acccgctgtt cttccctggc ggtaatatcg gcaagctggc
    22501 gatttgcggc accgccaatg acgttgcggt cagtggtgcc attccgcgct atctctcctg
    22561 tggctttatc ctcgaagaag gattgccgat ggagacactg aaagccgtag tgaccagcat
    22621 ggcagaaacc gcgcgcacgg caggcattgc catcgttact ggcgatacta aagtggtgca
    22681 gcgcggcgcg gcagataaac tgtttatcaa caccgcgggc atgggcgcaa ttccggcgaa
    22741 tattcactgg ggcgcacaga cgctaaccgc aggcgatgta ttgctggtta gcggtacact
    22801 cggcgaccac ggggcgacta tccttaacct gcgtgagcag ctggggctgg atggcgaact
    22861 ggtcagcgac tgcgcggtgc tgacgccgct tattcagacg ctgcgtgaca ttcccggcgt
    22921 gaaagcgctg cgtgatgcca cccgtggtgg tgtaaacgcg gtggttcatg agttcgcggc
    22981 agcctgcggt tgcggtattg aaatttctga atcagcgctg ccggttaaac ctgccgtgcg
    23041 cggcgtttgc gaattgctgg gactggacgc cctgaacttt gccaacgaag gcaaactggt
    23101 gatcgccgtt gaacgcaacg cggcagagca agtgctggca gcgttacatt cccatccact
    23161 ggggaaagac gcggctctga ttggtgaagt ggtggaacgt aaaggtgttc gtcttgccgg
    23221 tctgtatggc gtgaaacgaa ccctcgattt accacacgcc gaaccgcttc cgcgtatatg
    23281 ctaataaaat tctaaatctc ctatagttag tcaatgacct tttgcaccgc tttgcggtgc
    23341 tttcctggaa gaacaaaatg tcatatacac cgatgagtga tctcggacag caagggttgt
    23401 tcgacatcac tcggacacta ttgcagcagc ccgatctggc ctcgctgtgt gaggctcttt
    23461 cgcaactggt aaagcgttct gcgctcgccg acaacgcggc tattgtgttg tggcaagcgc
    23521 agactcaacg tgcgtcttat tacgcatcgc gtgaaaaaga cacccccatt aaatatgaag
    23581 acgaaactgt tctggcacac ggtccggtac gcagcatttt gtcgcgccct gatacgctgc
    23641 attgcagtta cgaagaattt tgtgaaacct ggccgcagct ggccgcaggt gggctatacc
    23701 caaaatttgc tcactattgc ctgatgccac tggcggcgga agggcatatt tttggtggct
    23761 gtgaatttat tcgttatgac gatcgcccct ggagcgaaaa agagttcaat cgtctgcaaa
    23821 catttacgca gatcgtttct gtcgtcaccg aacaaatcca gagtcgcgtc gttaacaatg
    23881 tcgactatga gttgttatgc cgggaacgcg ataacttccg catcctggtc gccatcacca
    23941 acgcggtgct ttcccgcctg gatatggacg aactggtcag cgaagtcgcc aaagaaatcc
    24001 attactattt cgacattgac gatatcagca ttgtcttacg cagccaccgt aaaaacaaac
    24061 tcaacatcta ctccactcac tatcttgata aacaacatcc cgcccacgaa cagagcgaag
    24121 tcgatgaagc cggaaccctc accgaacgcg tgttcaaaag taaagagatg ctgctgatca
    24181 atctccacga gcgggacgat ttagccccct atgaacgcat gttgttcgac acctggggca
    24241 accagattca aaccttgtgc ctgttaccgc tgatgtctgg cgacaccatg ctgggcgtgc
    24301 tgaaactggc gcaatgtgaa gagaaagtgt ttaccactac caatctgaat ttactgcgcc
    24361 agattgccga acgtgtggca atcgctgtcg ataacgccct cgcctatcag gaaatccatc
    24421 gtctgaaaga acggctggtt gatgaaaacc tcgccctgac cgagcagctc aacaatgttg
    24481 atagtgaatt tggcgagatt attggccgca gcgaagccat gtacagtgtg cttaaacaag
    24541 ttgaaatggt ggcgcaaagt gacagtaccg tgctgatcct cggtgaaact ggcacgggta
    24601 aagagctgat tgcccgtgct atccataatc tcagtgggcg taataatcgc cgcatggtca
    24661 aaatgaattg cgcggcgatg cctgccggat tgctggagag cgatctgttt ggtcatgagc
    24721 gtggggcttt taccggtgcc agcgcccagc gtatcggtcg ttttgaactg gcggataaaa
    24781 gctccctgtt cctcgacgaa gtgggcgata tgccactgga gttacagccg aagttgctgc
    24841 gtgtattgca ggaacaggag tttgaacgcc tcggcagcaa caaaatcatt cagacggacg
    24901 tgcgtctaat cgccgcgact aaccgcgatc tgaaaaaaat ggtcgccgac cgtgagttcc
    24961 gtagcgatct ctattaccgc ctgaacgtat tcccgattca ccttccgcca ctacgcgagc
    25021 gtccggaaga tattccgctg ctggcgaaag cctttacctt caaaattgcc cgtcgtctgg
    25081 ggcgcaatat cgacagcatt cctgccgaga cgttgcgcac cttgagcaat atggagtggc
    25141 cgggtaacgt acgcgaactg gaaaacgtca ttgagcgcgc ggtattgcta acacgcggca
    25201 acgtgctgca gctgtcattg ccagatattg ctttaccgga acctgaaacg ccgcctgccg
    25261 caacggttgt cgctcaggag ggcgaagatg aatatcagtt gattgttcgc gtgctgaaag
    25321 aaactaacgg cgtggttgcc gggcctaaag gcgctgcgca acgtctgggg ctgaaacgca
    25381 cgaccctgct gtcacggatg aagcggctgg gaattgataa atcggcattg atttaactgc
    25441 aaattgccgg acagatctga ctgtccggca tactattcac gaggtttttt cggacgatat
    25501 ttttccggca gttctggcac cggacgcttg tcatcgatga gatgacgcac ggttaagatc
    25561 ggatgacgcc acagcattct cggcccggcc caacgcataa tctgtttcat ctcttcacgc
    25621 tttgcaggct ggtaacagtg caccggacac tgcttacagg ctggcttctc ttcgccgaac
    25681 acacatttat ccagccgctt ttgcgcgtaa acaaacaacg cctcgtaatg ctccggctcc
    25741 gctgacgcct gcgggcattt cgcttgataa agatcgatca tttttttaat cgtcagtttt
    25801 tcacgagaga tacgcttgcc ggacatgctg cctccatact cttaaggtgt atttatacta
    25861 caacttaatt tgaaagggga ctatggctct gaaggaggag gtttattcaa ccgacgcaat
    25921 tttgcgtact tgcttcataa gcatcacgca aaagctgcaa aacagcatct ttcccggaac
    25981 caacatcaag aactcgccgt tcgcttcttc ccctgaaatg attaactccg gtatcatgtg
    26041 cgccttatgt gattacaacg aaaataaaaa ccatcacacc ccatttaata tcagggaacc
    26101 ggacataacc ccatgagtgc aatagaaaat ttcgatgccc atacgcccat gatgcagcag
    26161 tatctcaagc tgaaagccca gcatcccgag atcctgctgt tttaccggat gggtgatttt
    26221 tatgaactgt tttatgacga cgcaaaacgc gcgtcgcaac tgctggatat ttcactgacc
    26281 aaacgcggtg cttcggcggg agagccgatc ccgatggcgg ggattcccta ccatgcggtg
    26341 gaaaactatc tcgccaaact ggtgaatcag ggagagtccg ttgccatctg cgaacaaatt
    26401 ggcgatccgg cgaccagcaa aggtccggtt gagcgcaaag ttgtgcgtat cgttacgcca
    26461 ggcaccatca gcgatgaagc cctgttacag gagcgtcagg acaacctgct ggcggctatc
    26521 tggcaggaca gcaaaggttt cggctacgcg acgctggata tcagctccgg gcgttttcgc
    26581 ctgagcgaac cggccgaccg cgaaacgatg gcggcagagc tgcaacgcac taatccggcg
    26641 gaactgctgt atgcagaaga tttcgccgag atgtcgctga ttgaaggccg tcgcggcctg
    26701 cgccgtcgcc cgctgtggga gtttgaaatc gacaccgcgc gccagcagtt gaatctgcaa
    26761 tttggcaccc gcgatctggt cggttttggc gtcgagaacg cgccgcgcgg actttgtgct
    26821 gccggttgtc tgttgcagta tgcgaaagat acccaacgca cgaccctgcc gcatattcgt
    26881 tctatcacca tggaacgtga gcaggacagc atcattatgg atgccgcgac acgtcgtaac
    26941 ctggaaatta ctcagaacct ggcgggcggt gcggaaaata cgctggcttc tgtgctcgac
    27001 tgcaccgtca cgccgatggg tagtcgtatg ctgaaacgct ggctgcatat gccagtgcgc
    27061 gatacccgcg tgttgcttga gcgccagcaa actattggcg cattgcagga tttcaccgcc
    27121 gggctacagc cggtactgcg tcaggtcggc gacctggaac gtattctggc acgtctggct
    27181 ttacgaactg ctcgcccacg cgatctggcc cgtatgcgcc acgctttcca gcaactgccg
    27241 gagctgcgtg cgcagttaga aactgtcgat agtgcaccgg tacaggcgct acgtgagaag
    27301 atgggcgagt ttgccgagct gcgcgatctg ctggagcgag caatcatcga cacaccgccg
    27361 gtgctggtac gcgacggtgg tgttatcgca tcgggctata acgaagagct ggatgagtgg
    27421 cgcgcgctgg ctgacggcgc gaccgattat ctggagcgtc tggaagtccg cgagcgtgaa
    27481 cgtaccggcc tggacacgct gaaagttggc tttaatgcgg tgcacggcta ctacattcaa
    27541 atcagccgtg ggcaaagcca tctggcaccc atcaactaca tgcgtcgcca gacgctgaaa
    27601 aacgccgagc gctacatcat tccagagcta aaagagtacg aagataaagt tcttacctca
    27661 aaaggcaaag cactggcact ggaaaaacag ctttatgaag agttgttcga cctgctgttg
    27721 ccgcatctgg aagcgttgca acagagcgcg agcgcgctgg cggaactcga cgtgctggtg
    27781 aacctggcgg aacgggccta taccctgaac tacacctgcc cgacctttat tgataaaccg
    27841 ggcattcgca ttaccgaagg ccgccatccg gtggttgaac aggtgctgaa cgagccattt
    27901 atcgccaacc cgctgaatct gtcaccgcag cgccggatgt tgattattac cggcccgaac
    27961 atgggcggta aaagtaccta tatgcgccag accgcactga ttgcgctgat ggcctacatc
    28021 ggcagctatg taccggcaca aaaagtcgag attggaccta tcgatcgcat ctttacccgc
    28081 gtaggcgcgg cagatgacct ggcttctggg cgctcaacct ttatggtgga gatgactgaa
    28141 accgccaata ttttacataa cgccaccgaa tacagtctgg tgttaatgga tgagatcggg
    28201 cgtggaacgt ccacttacga tggtctgtca ctggcatggg cgtgcgcgga aaatctggcg
    28261 aataagatta aagcgttgac gctgtttgct acccactatt tcgagctgac ccagttaccg
    28321 gagaaaatgg aaggcgtcgc caacgtgcat ctcgatgcac tggagcacgg cgacaccatt
    28381 gcctttatgc atagcgtgca ggatggcgcg gcgagcaaaa gctacggcct ggcggttgca
    28441 gcccttgcgg gcgtgccaaa agaggtgatc aaacgcgcgc ggcagaaact gcgtgagctg
    28501 gagagcattt cgccgaacgc cgccgctaca caagtggatg gtacgcaaat gtctttgctg
    28561 tccgtaccgg aagaaacctc gcctgctgtc gaggcactgg aaaacctcga tccggattca
    28621 ctcaccccgc gtcaggcgct ggagtggatt tatcgcttga agagtctggt gtaattataa
    28681 ttcccggtag aaaaatacta tcgggaatat tatctacagc tgacgaatca aataaaaata
    28741 ccatgtataa taggaaagct tattttacag ggtaaaacca tgccatctac acgctatcaa
    28801 aaaatcaatg cccatcacta tcgccatata tgggtcgttg gtgatattca tggtgaatat
    28861 cagttattac aatcccgctt acatcaactc tcttttttcc ccgaaaccga cttacttatt
    28921 tctgtcggcg ataatattga tcgtgggccg gagagtctta acgtcctgcg cctgctaaac
    28981 cagccctggt ttatctccgt taaaggcaac cacgaagcaa tggcgctgga tgcgttcgcg
    29041 accggcgatg gcaatatgtg gcttgccagc ggtggtgact ggtttttcga tttaaatgat
    29101 tcagagcaac aagaggcaat agatctgttg ctgaaatttc atcaacttcc acatattatt
    29161 gaaatcacta acgacaacat aaaatatgtc atcacacatg cagattatcc ggggaatgaa
    29221 tatctctttg gtaaagaaat agcggagatc gaattactct ggcctgttga tcgtgtgcag
    29281 aaatcgctaa atggcgagtt acaacaaata aacggcgctg attattttat atttggacat
    29341 atgatgtttg ataacattca gacgttcgct aaccagattt atattgatac cggatcgccg
    29401 aaaagtgggt ggctgtcatt ttataaaata aagtagtctc atgcttcttc tgtgaagcat
    29461 gagtaacccg gtgttattgc aggccattat tcatttttcg ctaccagcaa agagagatcc
    29521 tgcttcgcca gcacgcgact ggcactctcc ggcaaaccgt cgtctgtaat aatctgatca
    29581 aactcgctta atggtaacgc cagccatgtc gccacctgac catatttcgt cgcatcacag
    29641 accaaaactc gctggcggct ggcactggca atcgcccgtt tcaccgtgac tttatcttcc
    29701 gctggcgtag aaatcccccg cacactccat gacgatgcag aaataaaagc ctgatcaatc
    29761 atcaggctgc gcagcatggt cgcagcggct tccccgacac aggaacggtt ttcccgacac
    29821 actgcaccgc cagtgtgaat aattgtgcaa ttactgttgt cgagtaagta gtccgcaata
    29881 acgaaatcgt ttgtgaccac agtcagtgac tccatgtgaa tcagatgctg tgctatcgct
    29941 aacgtggtcg ttcccgcatc cagatagata caacttcccg gctgaacaag acttgccgcc
    30001 agcttgccaa tagccgcttt ttgcgtcatt gccagcgcag tttttacctg atgagaaggt
    30061 tcatgcgcca cgcgtcccgg agactggacg cctccggaca ccagcacaac ggctccctgc
    30121 tgctccagtt tttgtaaatc ccgacgaatg gtcatatgtg acacattcat tctgtccgtt
    30181 agttcagcaa tactgacaat gcctttttca gctaccatct caaggatgat ttggcgacgc
    30241 tctacgggta tcaacttttg ctccttcctt tgtcctgctg acattctacg ctatttgcct
    30301 gcgaaacgtg cgcggcgcaa ctaacgctta gttcacataa aataacacac aatgttaatt
    30361 tttgtgaatc agctcaccat atcgttatct tccagcgctt atattcacaa tatcaaacaa
    30421 aatatcactt aaattaacaa ggagagcaga tgaaaacggg atctgagttt catgtcggta
    30481 tcgttggctt agggtcaatg ggaatgggag cagcactgtc atgtgtccgc gcaggtcttt
    30541 ctacctgggg cgcagatctg aacagcaatg cctgcgctac gttgaaagag gcaggtgctt
    30601 gcggggtttc tgataacgcc gcgacgtttg ccgaaaaact ggacgctctg ctggtgctgg
    30661 tggtcaatgc ggcccaggtt aaacaggtgc tgtttggtga aacaggcgtt gcacaacatc
    30721 tgaaacccgg tacggcagta atggtttctt ccactattgc tagtgctgat gcgcaagaaa
    30781 ttgctaccgc tctggctgga ttcgatctgg aaatgctgga tgcgccagtt tctggtggtg
    30841 cagtaaaagc cgctaacggt gaaatgactg tcatggcctc cggtagcgat attgcctttg
    30901 aacgactggc acccgtgctg gaagccgttg ccggaaaagt ttatcgcata ggtgcagaac
    30961 cgggactagg ttcgaccgta aaaattattc accagttgtt agcgggcgta catattgctg
    31021 ccggagccga agcgatggca cttgcagccc gcgcggggat tccgctggat gtgatgtatg
    31081 acgtcgtgac caatgccgcc ggaaattcct ggatgttcga aaaccggatg cgtcatgtgg
    31141 tggatggcga ttacaccccg cattcagccg tcgatatttt tgttaaggat cttggtctgg
    31201 ttgccgatac agccaaagcc ctgcacttcc cgctgccatt ggcctcaaca gcattgaata
    31261 tgttcaccag cgccagtaac gcgggttacg ggaaagaaga cgatagcgca gttatcaaga
    31321 ttttctctgg catcactcta ccgggagcga aatcatgatc aagattggcg ttatcgccga
    31381 tgattttacc ggcgcgacgg atatcgccag ttttctggtg gaaaacggtc taccaacggt
    31441 acaaattaac ggtgttccaa caggtaaaat gccggaagca atcgacgcac tggtgatcag
    31501 cctgaaaacg cgctcctgtc cggtggttga agccacacag caatcgctgg cggctctgag
    31561 ctggttgcaa cagcaaggtt gcaaacagat ctatttcaaa tactgctcta ctttcgacag
    31621 tacggctaaa ggtaatatcg gcccggttac cgatgcatta atggatgctc tcgacacgcc
    31681 gtttacggtc ttctctccgg ccctgccggt caacggacgt acggtttatc aggggtattt
    31741 gttcgtaatg aatcaactgc tggccgaatc cgggatgcgc catcacccgg taaatcccat
    31801 gaccgacagc tatcttcccc gtctggttga agcgcaatcc acagggcgct gcggcgtcgt
    31861 ttcggcacat gttttcgaac aaggtgtgga tgccgttcgt caagagctgg ctcgcttaca
    31921 gcaagagggc taccgctacg cggtgcttga tgcgctgacc gaacaccatc tggaaattca
    31981 gggagaagcc ttgcgcgatg ccccactggt aacgggcggt tctggtctgg cgattggcct
    32041 ggcccggcag tgggcgcaag aaaacggtaa ccaggctcgc aaagcagggc atccgctcgc
    32101 tgggcgcggc gtagtgctct ccggttcatg ctctcaaatg accaaccgcc aggtggcaca
    32161 ttaccgtcaa attgcaccag cccgtgaagt tgatgtggca cgctgcctct caattgaaac
    32221 tctggccgct tatgcacacg aactggcaga gtgggttctg ggccaggaaa gtgtacttgc
    32281 tccactggtt tttgccaccg ccagcactga cgcattggca gcaattcaac agcaatacgg
    32341 tgcacaaaaa gccagtcagg cagtagaaac actgttttct caactagcgg cgcggttagc
    32401 agcggaaggc gtgacacgct ttattgtcgc aggcggtgag acctccggcg tagtcacaca
    32461 gagcctggga ataaaagggt ttcatattgg cccaaccatt tcccccggcg tgccgtgggt
    32521 aaacgcactg gataagcctg tctcactcgc ccttaaatct ggcaacttcg gtgatgaagc
    32581 ctttttttca cgagcccaaa gagagttttt atcatgagcg atttcgcaaa agtagagcag
    32641 tctttgcgag aggagatgac gcggattgcc agttcattct ttcagcgcgg ctatgcaacc
    32701 ggttcggctg gcaatctgtc gctgctttta cctgacggga atttactggc gacaccgaca
    32761 ggttcatgcc tgggcaatct cgatccgcag cggctttcca aagtcgccgc ggatggcgaa
    32821 tggttaagtg gtgacaaacc ctcgaaagag gtgctctttc atctggcgct gtatcgcaac
    32881 aatccgcgct gtaaagcggt ggtgcatttg cacagcacat ggtcgacggc gctttcctgc
    32941 ctgcaagggc tggacagcag caacgttatt cgtccgttca caccatacgt ggtgatgcgg
    33001 atgggaaatg tcccgctggt gccttattac cgaccgggcg ataaacgcat cgcacaggat
    33061 ctggcggaac tggcagcaga caatcaggct tttttactgg caaatcatgg cccagtggtt
    33121 tgcggtgaaa gcctgcaaga agccgccaac aatatggaag agctggagga aacggcaaag
    33181 ctgattttta ttctcggtga ccgcccgatc cgttatctga ccgcaggtga aattgcggaa
    33241 ttaaggagtt aatgcaatgc ctcgttttgc agctaattta tccatgatgt tcaccgaagt
    33301 tccttttatt gaacgcttcg ccgcagcgcg aaaagccggg ttcgatgctg tggaatttct
    33361 gtttccctat gactactcca ccctgcaaat ccaaaagcaa ctggagcaaa accatctgac
    33421 actggcgctg ttcaataccg ctcccggaga tattaatgcg ggggaatggg gattatccgc
    33481 ccttcccgga cgtgagcacg aagcacacgc agacatcgac ctggcactgg aatatgcatt
    33541 ggagctcaat tgtgaacaag tccatgtaat ggcaggcgtc gtgcccgctg gcgaagatgc
    33601 tgaacggtac cgggcagtat ttatcgataa tctccgctac gctgccgacc gctttgcgcc
    33661 acacggcaag cgaatattag ttgaagcact cagccccggc gttaagcctc attatctctt
    33721 ctccagccag tatcaggcac tggctattgt tgaagaagtt gcgcgagata acgtgtttat
    33781 tcaactagat acttttcatg cgcaaaaagt agatggcaac ctgacgcatt taatccgcga
    33841 ctacgccggg aaatatgcgc acgtacaaat tgccggacta cctgatcgac atgaaccgga
    33901 cgatggagaa atcaactatc cgtggctgtt ccgcctgttc gatgaggtgg gatatcaggg
    33961 ctggatcggt tgtgaatata aacctcgtgg cctcaccgaa gaaggacttg gctggtttga
    34021 cgcctggcgc taattcgtta aatccctaat tacaacgtac ccatacatcc ccccatctac
    34081 ggatggggta ggttaactat aactttcaga cagggtttcc catgtccaca attacattgt
    34141 tatgcattgc gttaactggc gtaatcatgc tgttgctgct ggtcatcaag gcaaaggtac
    34201 aaccattcgt tgctctgctc ctcgtcagcc tgttagtcgc acttgcggcg ggtataccgg
    34261 cgggcgaagt gggtaaagtg atgatcgccg ggatgggcgg tgtgcttggt tccgtcacta
    34321 ttattattgg tctgggcgct atgctggggc gtatgatcga acactctggt ggtgcagagt
    34381 cactggctaa ttatttcagt cgcaagttag gtgacaaacg aactatcgct gcgctgactc
    34441 tggcagcgtt cttcctcggt attcccgtct tctttgatgt cggctttatt attcttgcgc
    34501 caatcattta cggttttgcc aaggttgcca aaatatcgcc actcaaattt ggcctgcctg
    34561 tcgctgggat catgctaact gtacatgtgg cggtaccgcc gcatccaggc cctgtcgccg
    34621 cagcggggtt actccacgca gacatcggct ggctaaccat catcggtatt gcgatttcta
    34681 ttcccgtagg gattgttggc tactttgcag cgaaaataat caataagcgc caatatgcga
    34741 tgtcggtgga agtactggaa cagatgcaac tggctccggc cagtgaggaa ggcgcgacaa
    34801 aattaagcga taaaataaat ccaccgggcg tcgcgctggt cacctcgcta attgttattc
    34861 ctatcgcgat tatcatggcg ggtacggttt ccgcaacact gatgccgcct tcgcatcccc
    34921 tgcttggtac gctacagctg atcggctcac caatggtagc tctaatgatt gcgctggtgc
    34981 tggcattctg gttattggct ttacgtcgcg gctggagctt acaacacacc agcgacatta
    35041 tgggctcagc gcttcctact gcggcggtag tgattttggt tactggtgct ggaggggtat
    35101 ttggcaaagt gctggtggaa tcgggcgtcg gcaaagccct tgccaacatg ttacaaatga
    35161 ttgacctgcc tctgttacca gccgcattta ttatttcact ggcgctgcgt gcatcgcagg
    35221 ggtcagcaac cgtagcaata ctgacaaccg gcgggttact ctcagaagcg gtgatgggat
    35281 tgaatccgat tcagtgcgta ttggtgacgc tggcagcctg ctttggtggg cttggggcgt
    35341 cacatattaa tgactcaggg ttctggattg tgaccaaata tctggggttg tcggtagcag
    35401 acggtctgaa aacctggaca gtgttaacga ccattctcgg ttttaccgga ttcttaatta
    35461 cctggtgcgt atgggcggta atttgacctt aataaaaagg tccgatgagc atcggacctt
    35521 ttatttagcg cttaccttcc gccagcagcg gcgggatgct cggcaccatt ggcgcgtcat
    35581 caatatcttt ctgcgtcatg cggaacgctt cgggataatg ttcgcggctg gtacggcgca
    35641 gcggttcggt atcgcgccag gtataaaggc aatgctggca ctgatatacc gtccagacat
    35701 ctttcaccgg cgatttcgcc atcacttcaa tctgttcatc ggcacaacgt ggacaaatca
    35761 tcttgttctc cttatttacg tgcggccagc atagcggtca gcttttcagc ccaggctttg
    35821 gtttccggca aatccaccac cggctggctg tagtggccac ggttgtccgg ggcgacaggc
    35881 gtggtggcgt cgataatcag cttgtcggtg atccccgccg ggcttgagcc agggtcgagt
    35941 tccagcacgg acatattcgg caactgcacc aaatcccctg ccggatttac tttcgaggag
    36001 agcgcccaca tcacctgcgg caggttgaac gggtcaacgt cttcatcgac cataatcacc
    36061 atctttacgt agcccagacc gtgcggcgtg gtcatcgcac gcaggcccac cgcgcgggca
    36121 aagccgccgt agcgtttttt ggtggagata atcgccagca ggccgtgggt gtacatggcg
    36181 tttactgcct gcacttccgg gaactcggct ttcagttgtt gatacagcgg cacacaggtg
    36241 gctggcccca tcaggtagtc gatttcggtc cacggcatgc cgaggtacag cgattcgaaa
    36301 atcggcctgg tgcggtaaga gactttatcg atgcgcacca cggtcatgtt gcgcccgccg
    36361 gagtagtgcc cggtaaactc accgaacggc ccttcaattt cgcgtttacg gctttcgatg
    36421 accccttcga ggatcacttc tgaaccccac ggcacatcaa aaccggtcag cggggcggtg
    36481 gcgatcgggt acgggctttc acgcagcgcg cctgccattt catactcaga ctgatcgtat
    36541 ttcagcggcg tggcccccat cagcgtgatg atcggatcgt taccgagcgt gatcgcaacc
    36601 ggcagatctt caccgcgctc ttctgcttta tgcagatgca gggcgatatc gtgcatcggc
    36661 accggttgca ggccgagctt acgcttgccc ttcacttcca tgcggtagat gccgacgttc
    36721 tgcttgccga agttatctgg gtcgagcgga tcgcgggaaa ccacgcaggc tttgtcgaga
    36781 tagaaaccgc catcaccgtc gtttaaacga aacagcggca ggatatcgaa cagattaatc
    36841 tcgtcaccat cgacggtgtt ctgcgcccag gcaggattgg cgcggcgctc cggggcaatc
    36901 gggaagttat cccagcggcg gataaactca tcaatctgct ttttgaccgg ggtgtttggt
    36961 ggcaggccga gggaaatcgc gtggttctgc caggaaccga tggtattcat cgccacgcgg
    37021 gcatcggtaa agccgcgaat attatcaaac cacagcgccg gtgcaccatc gccgatacgc
    37081 ccggtggcgt tggcagcggc agccagatcc ggttccgcgt tcacctcttc gctgattttc
    37141 agtaactggc cgtggtcatc aagcgcctgt aaaaagctgc gtaaatcatc aaatgccatt
    37201 attcattctc ctgagaaaaa ttccgggcct gcggcaaccc ttgccagcgc ctggcgtggg
    37261 gatgttcgag gccaaattga tccagcacgc gggctaccac atggtggaca atgtcatcta
    37321 ccgtttcggg atggttataa aaggcaggca tcggcggcac catcgccacg cccatgcgtg
    37381 aaagtgcgag catattttcg agatggatgg tgctaagcgg catttcacgc ggcaccagca
    37441 ccagtttgcg gccttctttg agcacgacgt ccgccgcgcg ccctaccagg ccatcggcgt
    37501 aaccagcgcg gataccggcg agcgttttca tactgcacgg aataacgatc atgccgtcgg
    37561 tacgaaagga acctgaggag atggtcgccg cctgatccgc cgggttatgg ctgaagtctg
    37621 cgagggcagc aacatcgcga gcgctgtaag gcgtttccag ttcaatggtg gttttcgccc
    37681 acttcgacat caccagatga gtctcgacat tcggcatctc ccgcagcgct tgcagtaatg
    37741 ccacaccaag aggcgcaccg gtagcccctg tcatcccgac gatcagtttc attgcttatt
    37801 ctcttaattg ttcgtatacg aacgttatta ttaaaatacg cctctgttct cattgcggca
    37861 agcactgaaa agtaacaaca tccccttcgt cactgaaaaa ccgctatgat agcggcagat
    37921 agtttgcccg gagtttacat ggcgttacga aataaagcgt tccatctgtt acgacaactt
    37981 tttcagcagc acacggctcg ctggcagcac gagttacctg acctgaccaa accacagtat
    38041 gcggtgatgc gcgccattgc tgataagcct ggtatcgaac aggtggcact gatagaagca
    38101 gcagtcagca ccaaagcaac gttggcagaa atgttggcaa gaatggagaa tcgtggctta
    38161 gtcagacgtg aacatgacgc cgctgataag cggcgacgct ttgtctggct gactgccgaa
    38221 ggagaaaaga tacttgctgc ggcgataccg attggtgaca gcgtggatga ggaatttttg
    38281 gggcgtttga gtgcagaaga gcaggagctg tttgtgcagc tggtgcgcaa gatgatgaac
    38341 acataggatg caaattgccg ggtaggacgc tgacgtgtct tatccaggcg acaaaaacac
    38401 aagcttccta aacaaaagaa aaaggccagc ctcgcttgag actggccttt ctgacagatg
    38461 cttacttatt cgcggaacag cgcttcgata ttcagcccct gcgtttgcag gatttcgcgc
    38521 aaacggcgca ggccttcaac ctgaatctgg cgaacacgtt cacgggtgag gccaatttca
    38581 cgacctacat cttccagtgt tgccgcttcg taccccagca aaccgaatcg acgtgccagt
    38641 acttcacgct gtttggcgtt cagctcgaac agccatttga cgatgctctg cttcatatcg
    38701 tcatcttgcg tggtatcttc cggaccgttc tctttttcat cggccaggat gtccagcaac
    38761 gctttttcgg aatcaccacc cagcggggtg tctaccgagg taatgcgctc gttaagacga
    38821 agcatacggc tgacgtcatc aactggctta tccagttgct ctgcgatctc ttccgcactt
    38881 ggttcatggt ccagcttatg ggacaactca cgtgcggttc gcaggtaaac gttcagctcc
    38941 tttacgatgt gaatcggcaa acgaatagta cgggtttggt tcataatcgc ccgttcaatc
    39001 gtctggcgaa tccaccaggt tgcgtatgtt gagaagcgga aaccacgttc cgggtcaaac
    39061 ttctctaccg cgcggatcag ccccaggttg ccctcttcga taaggtccag caacgccaga
    39121 ccacgattgc cataacggcg ggcaattttt accaccagac gcaagttact ctcgatcatc
    39181 cggcggcgag aggcgacatc tccacgcagt gcgcgacgcg caaaataaac ttcttcttcg
    39241 gccgttaaca gtggtgaata accaatctca ccaaggtaaa gctgagtcgc gtccaacaca
    39301 cgctgtgtgg ctccctgcga taacagttcc tcttcggcca aatcgttatc actgggttcc
    39361 tcttctacta aggccttttc gtcaaaaacc tcaactccgt tctcatcaaa ttccgcatct
    39421 tcatttaaat catgaacttt cagcgtattc tgactcataa ggtggctcct acccgtgatc
    39481 ccttgacgga acattcaagc aaaagcctgg ttccgccgat ttatcgctgc ggcaaataac
    39541 gcagcgggtt tacggatttc cccttgtaac gaatttcaaa atgcaagcgt gttgaactgg
    39601 ttccggtgct acccatggtt gctatttttt gccccgcctt cacttcttgt tgttcccgga
    39661 ccagcattgt gtcgttatgg gcgtaggcac tcaggtaatc atcattatgt ttgatgataa
    39721 tcagattacc gtagccgcgc agcgcgttac cggcataaac aacgcggcca tctgcggtcg
    39781 cgataattgc ctgtcctttg ctgcctgcga tatcaatccc cttgttgccc ccctcagaag
    39841 cgccaaaggt ttcgatcact ttgccctcag tcggccagcg ccaggtggag ataggcgtac
    39901 tggttgatgt actgctgaca gtcggctcgg ttgtgcttgc tgttggtacc gttacaggcg
    39961 ctgtgaccgt ggtcgcagtt ggcttgttgt tcggcaacat tttgttagca ctctgttcac
    40021 ccgaagactc agaatacgta attgtcggtt gcgacgcaac agcaacggtg gaattttgtg
    40081 caggcttgat cacaactcct tgctctgctg cgtcggcctg ggtaatggca tttccgccag
    40141 tgattggcgt accggaagca ttccccacct gtaaggtctg accaacgttc agcgcgtatg
    40201 gtgcctgaat attgttgcgc tgagcaaggt cacggaaatc gttgccagta atccaggcga
    40261 tatagaaaag tgtgtcgcct tttttcacgg tgtatgtact gccgctataa ctgcctttcg
    40321 gaatgttccc atactgacgg ttgtagacga ttcgtccgtt ttccatctgt accggctgct
    40381 gagctactgg ctgcactggc tggatttgcg gttgttgagt agcctgaatt tgtggctgct
    40441 gtaccggctg aatttgcggt tgctgcgctg tagacgtcgt ccccattttc ggcggcggcg
    40501 taatcaacat accagaattg gtatttgcag gcgcattgcc attaacggag ctgaccggtg
    40561 ccggtggatt tgaagtgtca gaacagcctg ccagccatag cgtaaccagt gacaaagccg
    40621 caatgcggcg aacggtgaat tttgggcttc ccgcgctcat ttatccccca ggaaaaattg
    40681 gttaataacc agtgacataa ttaccgtgca aggcaccata ctgaacactg gaaaagatgt
    40741 tcacgatacg ctgacctgcg gcaaaataac caggaaaaat ccaggtattt cctcacgttt
    40801 taagccagct cacccttcac taaagggaca aagcgcacgg cctccacggt atcgataata
    40861 aattcgcctc cccgacgacg cacccgtttc aaatactggt gcttctcccc tacgggtaag
    40921 acgagaatcc cgccttcgtc cagctgcgtc attagcgcag ttggaatttc cggcggtgcc
    40981 gccgtaacaa tgatagcgtc aaacggcgca cgtgcctgcc aaccttgcca tccatcgcca
    41041 tgacgggttg aaacattatg taaatcaaga tttttcagac ggcgacgcgc ctgccactgc
    41101 aaacctttaa tccgttcgac cgagcaaaca tgctggacaa gatgcgccag gattgccgtt
    41161 tgatatcccg aaccggtgcc aatttccagc acccgcgact gcggcgtcaa ctcgagtaat
    41221 tcggtcattc gcgccaccat atacggctgc gaaattgtct gcccctgacc tatcggcaag
    41281 gcgatattgt cccaggcttt ttgttcaaac gcttcatcaa cgaatttttc acgcggcacg
    41341 gcggcaagtg cattcagcac ctgctcatcc tgaatacctt gcgcacgtaa ttgatccaga
    41401 agtgcttgta cgcgtctgct taccattgcg tgccaactcc cacgctgttt aaccagtctg
    41461 aaaccacatc ttgcgcgcta tgcgcagtta aatccacatg cagcggcgtg atggagacat
    41521 agccctcatc taccgcagca aaatcggtcc ccggaccggc atcacattta ccgcccggcg
    41581 ggccaatcca gtacagcgta ttgccgcgcg gatcttgctg cgggatcacc tgatctgccg
    41641 gatgtcgtgt accgcagcgc gtcacgcgaa tacctttgat ttgatccaag ggtaaatccg
    41701 gaacgttaat attaagaata cgcccggtgc gcagcggctc tttacacagt gcgcgcaaaa
    41761 ttgaacaggt taccgccgcg gcagtgtcgt aatgtttatg cccgtcaagc gagacggcaa
    41821 gcgccggaaa acctaaatga cggccttcca tcgcggcggc taccgtaccg gaataaataa
    41881 catcatcccc cagattcggc ccggcgttaa ttccggacac aacaatgtcc gggcgcggac
    41941 gcatcagagc attcacgcca agatagacgc aatcggtcgg ggttcccatt tgcacagcaa
    42001 tatcaccatt ttcaaaggta aacgtgcgca gggaggattc cagtgtcaga gaatttgaag
    42061 cgccgctgcg gttacgatcg ggggcgacca cctgaacgtc agcaaactca cgcaaggctt
    42121 tcgccagcgt ttgtataccg ggtgcatgta ccccgtcatc attactcagc aatatgcgca
    42181 taatcacctg ttgtgttgat aagttccctg acaacgctgg ttgcaaaact acccgccgga
    42241 agccagaaac ggatctctac ggtgacgtca tcccaccaat tccagcttaa ttgttgcgga
    42301 tacagcagca tcgctctgcg cgcggcttca actttttcgc gcaccagtaa agcttgtaat
    42361 tcagtttctg cggcgacagc tgcttgttcg aatgccagcg cttcacgctg agttccccat
    42421 tcgccactgc ccggcaatgc ggcggttatc atcagctctt tatcgttgac gcgacgctgt
    42481 aattccgcca gttcttcggt ggttgctaca aaccagctac cacgtccggc taattgtagc
    42541 gcatcgccgt caacaacttg attaacgtct gcttttttga ggcgctcagc aacaatctga
    42601 ttaaacaacg cactgcgggc tgccgacaac caaaaactcc gtttattgcg atcgcgcacc
    42661 ggagtattgg tttgcgccca gcgcagcgcg ccctgcaagt tgctaccgcc aatcccaaaa
    42721 cgttgggcac cgaagtagtt cggtacacct tttacgcaaa tatcgatcag acgttgttca
    42781 acgtcatcgc gattgctcac ttcgcgtagt accagggtaa aggcgttacc tttcagcgcc
    42841 cctaaacgca gcttgcgctt gtgccgcgca tactccagca cctggcagcc ttccagttga
    42901 aaggcgctca gatcgggcat ctctttgccc ggcacgcgag cgcataacca ctgttccgtt
    42961 acagcatgtt tgtctttttg cccagcgaag ctgacttcac gggaatgaat tttcaggaat
    43021 ttcgccagtg catccgccac aaaacgggta ttgcagccgt ttttgaggat tctaaccaga
    43081 atatgctcac cttcaccatc aggctcaaag cccaaatctt ccaccaccac aaagtcttcc
    43141 ggattggctt tcagcagccc ggtgccttgc ggtttaccgt ggaggtaagt gagattatca
    43201 aactcaatca ttttgttgcc ttaatgagta gcgccaccgc ttcacaggca atcccttccc
    43261 cacgtccggt aaatccaagt ttttccgtag tagtggcttt cacgttaaca tcatccatat
    43321 gacagccgag atcttcggca ataaacacgc gcatttgtgg aatgtgcggc aacatcttcg
    43381 gtgcctgagc gatgatagtg acatcgacgt tgccaagggt ataacccttc gcctgaatac
    43441 gacgccaggc ttcgcgtagc agctcgcggc tgtcggcacc tttaaatgcc ggatcggtat
    43501 ccgggaacag tttgccgata tcccccagcg ccgccgcgcc aagcaatgca tcggtcaacg
    43561 catggagcgc cacgtcgcca tcagaatgcg ccagcaatcc tttttcgtaa ggaatgcgta
    43621 cgccaccaat gataattggg ccttcaccgc caaaggcgtg tacgtcaaaa ccgtgtccaa
    43681 ttcgcattat gtattctcct gatggatggt tcgggtgagg taaaactcgg ccagtgccaa
    43741 atcttccggg cgcgtgactt taatgttatc cgcgcggcct tcgaccaact gcggatgaaa
    43801 tccgcaatat tccagcgccg aggcttcgtc ggtaatagtc gcgccttcat ttagagcgcg
    43861 cgtcagacag tcatgtaaca gctcacgagg gaaaaattgc ggcgtcagcg cgtgccataa
    43921 gccgttgcga tcaacggtat gagcaatggc atttttgccc ggttcggcac gtttcatagt
    43981 atcgcgcact ggtgcggcta ggatccctcc cgtgcggctg gtttcgctca acgccaacaa
    44041 tcgcgcgagg tcatcctgat gcagacaagg acgagcggcg tcatgcacca atacccactg
    44101 cgcgtcgcca gcggctttca aacctgccag cacggaatcg gcacgctcat caccgccatc
    44161 tacaacggtg atttgcggat gattcgccag aggaagttgt gcaaaacggc tatcgccagg
    44221 acttatggca atgacgacac gtttcacccg gggatgcgcc agcagcgcat gcaccgagtg
    44281 ttcaagaatg gtttgattac cgattgagag atattgctta ggacattccg tttgcattcg
    44341 acggccaaat ccggccgccg gaaccacggc gcaaacatcc aaatgagtgg ttgccatgtt
    44401 aattcccggg ctgatttatc gattgttttg ccccgcagac tgtgcgcgct tcgacgcgtc
    44461 aggcaccaga cgataaaaag tttcgcccgg cctggtcatg ctgagttcat tacgcgcacg
    44521 ctcttcgagc gcctcctggc cgccattgag atcgtcaatt tcggcaaaaa gttgatcgtt
    44581 tcgcgcttta agtttcgcgt ttgtagcttg ctgtgccgcc acatcatcat tgacgcgggt
    44641 atagtcatgt ataccgttct taccgaacca cagcgaatac tgtagccaga ccagaatagc
    44701 cagcaacagc agcgttagtt tacccatcct gccccctgaa aaacggcatc atcatcccat
    44761 gcatccgaag acgactctac atcctctgtt ggggataccg cgacaacgcg ggcaaatgta
    44821 ccacatttgt ccattgttac gtatacccag ggcgtgcaga acataatctc attattagtt
    44881 acggtttgaa ttatgaacag aggagacaag aaagtacaaa ttagcccagt agccacataa
    44941 acagtgcgcc aaacataatg cctactgtca tcagggtgaa aacaatactg tagcgtagct
    45001 ttccgtccat caatgaatgc agcgcaatcc ccaccactac cgcgacgggc atcagcgcca
    45061 gaaagaaagg ccaggtgtag ataaagaaga acagcgtgtt agagccataa atcaacatcg
    45121 gcatcgccag cgcaaataac caggagataa aaccgaccac ggcaccaggc agtgaccatg
    45181 tggtttcttc atcctcagta aggctgtcgt tatttgttag tgtaatgtta tggctattac
    45241 gcatatttga tcctgttact ttgacgaacc gggcatggaa acccggtggt gtctcaggat
    45301 ctgataatat cgttctgtct caacagatct aataattgct gtaccaaatt tgttactaat
    45361 tgttcaccat tgagatgaat ttctgccgat tcaggcgctt cgtaaacaga atctattccc
    45421 gtaaagttgc gcagttcacc ggcacgcgct ttcttatata agccttttgg atcgcgggct
    45481 tcgcaaatcg ccagcggcgt atcgacaaac acttcgataa agcgcccttc tcctacgcgt
    45541 tcgcgaacca tctggcgttc ggcgcggtgt ggcgagataa atgcggtcag cactaccagt
    45601 ccggcttcaa ccatcaaatt cgccacttca ccgacgcgac ggatattctc tttacgatcg
    45661 gcatcgctaa aaccgagatc gctgcataat ccgtggcgaa cattatcgcc atccagcaga
    45721 tacgtactga cgccgagttt atgtaacgcc tcctccagcg ccccggcgac cgttgattta
    45781 ccggacccgg agaggccggt aaaccacagc actacaccac gatgaccgtg gtgtagctcg
    45841 cgttgttgca cagtgaccgg atggctatgc cagacgacgt tttcgtcatg cagcgccatt
    45901 atttatcccc cagcaaatcg cgcgcgcccc agtgcggaaa gtggcgacga accagagcat
    45961 tcaattccag ttcgaatgca ctgaattcag atggcgcagc agttgcctgg ctaactggct
    46021 cgtgcaccat accggcaccc acggtcacat tgctcaggcg atcgataaaa atcagcccac
    46081 ccgtcaccgg attttgttga taacgatcta acaccagcgg ctcgtcaaaa gtgagatcca
    46141 cgaggccgat cccattcagt ggcaggtttt caacttcacg ctgggtaagg ttattaatat
    46201 caacctgata gcgaatgcca tcaacacgcg cgcgcgtctt cttaccggca attttgatgt
    46261 cgtaactctg ccctggagaa agcggctgtt ccgccatcca taccacatcc accgacgcgc
    46321 tctgcaccgc cggtaacgct tcgtctgccg ccagcagcag atcgccacgg ctgatgtcga
    46381 tctcatccgt cagcaccagg gtgatcgctt ctccggcaaa ggcttcttcg cgatcaccat
    46441 caaaagtcac gatccgcgcg acgtttgatt ccacaccaga gggcagcact ttgacacgtt
    46501 gcccgacttc cacgcgaccg gatgccagcg ttccggcgta gccacgaaaa tcgagattcg
    46561 ggcggttaac gtactgcacc gggaagcgca ttggctgagc atccaccact cgctggatct
    46621 ccacggtttc cagcacttcg agcagtgtag gaccgctgta ccacggcata ctttcacttt
    46681 gtgaagccac gttgtcacct tccagtgcgg agagcggcac aaagcggata tccagattcc
    46741 ccggcagttg cccggcaaaa gtcagataat cttcacgaat acgtgtgaac gtctcttcac
    46801 tgtaatccac cagatccatt ttgttgatcg ctacgaccag atgtttgatc cccagcagcg
    46861 tggagataaa actgtgacga cgggtttgat cgagcacgcc tttacgggca tcgatcagta
    46921 agatcgccag ctcacacgtt gaggcgccgg tcgccatatt gcgggtatac tgctcgtgcc
    46981 cgggggtgtc ggcgataata aatttacgct tctcggtaga gaaatagcgg taggccacat
    47041 caatagtgat gccctgttcg cgctcagctt gcaggccgtc caccagcaga gccagatcca
    47101 gtttttcgcc ctgggtgccg tgacgcttac tgtcgttatg cagcgatgag agctgatctt
    47161 cgtagatttg gcgggtatcg tgcagcaggc gaccaatcag ggtacttttg ccgtcatcga
    47221 cgctaccaca ggtcagaaaa cgcagcaggc ttttatgttg ttgcgcaatc atccaggctt
    47281 cgacgccgcc ttcattggcg atttgttgtg caagtgcggt gttcatctta aaaatacccc
    47341 tgacgttttt tcagctccat agaccccgcc tggtcgcggt caatcacgcg gccctgacgt
    47401 tcactggtgg tagaaaccag catctcttcg atgatctccg gcagtgtttg tgcatttgac
    47461 tccaccgcac cggtcagcgg ccagcagccc agcgtacgga aacgcaccat ccgtttttta
    47521 atcacttcgc ccggttgcag gtcgatacgg ttgtcatcaa tcatcatcaa cataccgtcg
    47581 cgttccagaa ccggacgttc cgcagcgaga tacaacggca caatgtcgat attttccagc
    47641 cagatgtatt gccagatatc ctgctcggtc cagttagaga gcgggaaaac gcggatgctt
    47701 tcgcctttgt taatctgccc gttatagttg tgccacagct ccgggcgctg atttttcggg
    47761 tcccagcgat ggaagcgatc acggaaagag tagatacgct ctttagcacg ggatttctct
    47821 tcgtcgcggc gcgcaccacc gaaggcggca tcaaaaccgt atttattcag cgcctgcttc
    47881 aggccttcag ttttcataat atcggtatgt ttcgcgctgc cgtgcacgaa tggattaatc
    47941 cccatcgcca cgccttccgg atttttatgc accagcagct cgcagccgta ggctttcgcc
    48001 gtacgatcgc ggaactcata catctcgcgg aatttccagc cggtatcgac atgcagcaac
    48061 gggaaaggca gcgtacctgg ataaaacgcc ttgcgcgcca gatgcagcat gacgctggaa
    48121 tctttaccga tagagtagag catcaccgga tttgagaatt ctgccgccac ctcgcgaata
    48181 atgtggatgc tttccgcctc cagttgccgc aggtgagtaa gtcgtatttg atccataacc
    48241 gttcctttgc aataccgcta ttttcttgcc atcagatgtt tcgactatag ggagcgtaag
    48301 agaacgaatg aaattaccaa ttagaatgag tagttcctta acggaataac gatttggcaa
    48361 agctaatatc aaaaagtgct taaggcaccg gatttcgggc gtttaggaat atttgaaatt
    48421 gttttagcgc agcggcagtt tcatactatg gcggtaaaaa aatttgcatg gtatttaagg
    48481 actcactatg ttttccgcat tgcgccaccg taccgctgcc ctggcgctcg gcgtatgctt
    48541 tattctcccc gtacacgcct cgtcacctaa acctggcgat tttgctaata ctcaggcacg
    48601 acatattgct actttctttc cgggacgcat gaccggaact cctgcagaaa tgttatctgc
    48661 cgattatatt cgccaacagt ttcagcaaat gggttatcgc agtgatattc ggacatttaa
    48721 tagtcggtat atttataccg cccgcgataa tcgcaagaac tggcataacg tgacaggaag
    48781 tacggtgatt gccgctcatg aaggcaaagc gccgcagcag atcatcatta tggcgcatct
    48841 ggatacttac gccccgctga gcgatgctga cgccgatgcc aatctcggcg ggctgacgtt
    48901 acaaggaatg gatgataacg ccgcaggttt aggtgtcatg ctggaattgg cagaacgcct
    48961 gaaaaatacg cctaccgagt atggtattcg atttgtggcg accagcggcg aagaggaagg
    49021 gaaattaggc gctgagaatt tactcaagcg gatgagtgac accgaaaaga aaaatacgct
    49081 gctggtgatt aatctcgata acttaattgt tggcgataaa ttgtatttca acagcggtgt
    49141 aaaaacccct gaagcagtaa ggaaattaac gcgcgacagg gcgctggcaa ttgcgcgcag
    49201 tcacggaata gccgcaacga ccaatccggg tttgaataaa aattatccga aaggcactgg
    49261 gtgttgtaat gacgcagaaa tattcgacaa agcgggcatt gctgtacttt cggtggaagc
    49321 gactaactgg aatcttggga ataaggatgg ttatcagcaa cgcgcaaaaa cacctgcctt
    49381 cccggcggga aatagctggc atgacgtaag actggataat caacaacata ttgataaggc
    49441 acttcctgga agaatagaac gtcgctgccg tgatgttatg cggataatgc taccgctggt
    49501 gaaggagctg gcgaaggcgt cttgatgggt ttgaaaatgg gagctgggag ttctaccgca
    49561 ggggcgggga attctaagtg atatccatca tcgcatccag tgcgcccggt ttatccccgc
    49621 tgacgcgggg aacaccagat tatcttcttt ttcaatccag ccaccgacgg tttatccccg
    49681 ctggcgcggg gaacacgatt gttttcgcgc ccgtgtcatt gtcgaggtcg gtttatcccc
    49741 gctggcgcgg ggaacacggc aacataacga acaaaatcaa cgtcaacctc ggtttatccc
    49801 cgctggcgcg gggaacactt gctgaaaaag aaggctccgg cgttatcagt cggtttatcc
    49861 ccgctggcgc ggggaacact agttaacttt tagacagaat atccgtgtac ccggtttatc
    49921 cccgctggcg cggggaacac gtcacgcttt aactgaccat taatatcgcg gatatgccgc
    49981 tgtaatcgtc ctgctcgctg aatcagcaaa tcaacagggg ccaggtcgga gatcatttcg
    50041 tccaggtcaa tatccacgct ttgctcaatt acttgtgtac aaacaatgac ttgcgacgct
    50101 ctctgtaaac tacaatattt accaaagcgt gccagcgttt ccgtttcaat tcgctggcga
    50161 tcgctaaaag caaagcggct atgaaaaagt gaaaggctgg aagcaggaat gacgccgcgg
    50221 gcaagcagct gacgatgaac cttaatagcg tcatcgacag aattccggat ccaggcgatg
    50281 catttcccct gacttaccgc cgattcgata cgcgcaatac actcttgttc actatgaagc
    50341 caacccacgc tgacgctacg ctcaacgtct ttgcgcgtcg ctacccggtg tgagttcaca
    50401 tcggatttcg tgacatgcgt cagccagggg taatcatcct tttccaggaa cggagcttct
    50461 tgctggccct ctgtaccccg cgcaaaggca gcgacgagtt tgtcgcgctg ctgttgagat
    50521 aacgtggcag aaagcaaaat aacgctgttt ccgccacgcg cctgccgctc gatcaaccct
    50581 tcaagaatgc acgacatgta agcgtcacag gcgtggatct catcagccag caggattttg
    50641 ttactcagcc ccagaagccg cagattatta tgtttgaacg gcatcaccgc cattatcgcc
    50701 tgatccagcg tgccgacgcc aatttcagcc agcaacgcct tcttgttact gtcggcaaac
    50761 caggccgcac atccctgact gaacgtttgt tcatccggtt cttctgaccc gactaaatca
    50821 ccagaccaga gtgattcatt aaagcggtcc attaatgtgc gggcactatg tgccagcacc
    50881 aggctggggc gggattctgg cgaatagaaa gcaagccagg ttttgaccag ccgatcgtac
    50941 atggcattgg ccgttgccat tgttggcaga ccaaaaaaca gcccctgagc tttccccgca
    51001 gctatcaacc tgtgcgccag gataagcgcc gcttctgttt tacctgcgcc agtcacatct
    51061 tccagaataa ataactgcgg tccttgctgg ctgatatcca gatccagcac cttttgctgt
    51121 aacggtgtcg gattctcaat aaaaggaaac agcgtattaa ttccgttgaa aggcgcgact
    51181 tttgctttta gaggaaagac ggttaaggcg ttttgagcct gaattaaagt tttctgccag
    51241 taacctttaa tatccattgg gtgtgcgacg cgtggaaaaa atcgcgttga cgaacccgtc
    51301 cagtctgcga gcacgacggt tgcagagata taccaggaaa ggtgttttat aagttcaatg
    51361 ccctcatcat catcccagaa tgcggggatc tctatgagcg gaaacagtgg tttgatttca
    51421 aggagaaaat ctcgtgctgc agctttgtct tcaggcagaa aattatccaa ttcatccata
    51481 cgatcagggg gccgaccatg atgccctgtc gtcacgggca tccacatttc tatcactcgc
    51541 ctaagtttgc gcggagagag cgaagatgaa ggaagtaact cctgacattc acttaaataa
    51601 tgattccaca accagtagcc cagcgttgaa tgagagattt tttcgtaatt ttttctggca
    51661 ctttcctgaa tcttgagttc agggggcagg taaagttgtt gaaaagagcg ggcaaatttt
    51721 ccaatatcgt gccagcacag caaccaggcg aaaaattgag ccgcctgttc cttgtcagaa
    51781 atccctaatt gacgaaaata atcagccagc ccgaagcaat ttcttttaac cattaaatag
    51841 cccatcgcgg ctacatccag cgaatgccag caaagaaggt gatagtcgtc gcccccgtct
    51901 ttctcgccgc gtcgggtttt tccccagaaa tcaaaaaaag tcac
//
LOCUS       DAPGOF010000009        50568 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.009, whole genome shotgun sequence.
ACCESSION   DAPGOF010000009 DAPGOF010000000
VERSION     DAPGOF010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.009"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..280
                     /locus_tag="RHV13_000455"
     CDS             <1..280
                     /locus_tag="RHV13_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDV1240016.1"
                     /translation="NNVKVAADAFLEILYSLKNKYCIKLLSAQDRAFIYEFARMIFAY
                     INDKSIEILLLSCFAAADQKAIQRYIPQAQDGEDFRNHLQYKLTLPTH"
     gene            491..814
                     /locus_tag="RHV13_000456"
     CDS             491..814
                     /locus_tag="RHV13_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDV1240017.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            811..1641
                     /gene="amiD"
                     /locus_tag="RHV13_000457"
     CDS             811..1641
                     /gene="amiD"
                     /locus_tag="RHV13_000457"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HDV1240018.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(1638..2651)
                     /locus_tag="RHV13_000458"
     CDS             complement(1638..2651)
                     /locus_tag="RHV13_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDV1240019.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(2750..4180)
                     /locus_tag="RHV13_000459"
     CDS             complement(2750..4180)
                     /locus_tag="RHV13_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HDV1240020.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDSAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKRIAKLAEQSTD"
     gene            complement(4191..5192)
                     /gene="ltaE"
                     /locus_tag="RHV13_000460"
     CDS             complement(4191..5192)
                     /gene="ltaE"
                     /locus_tag="RHV13_000460"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HDV1240021.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMTGGGMRQSGILAAAGIYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(5229..6947)
                     /gene="poxB"
                     /locus_tag="RHV13_000461"
     CDS             complement(5229..6947)
                     /gene="poxB"
                     /locus_tag="RHV13_000461"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HDV1240022.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(7080..8048)
                     /gene="hcr"
                     /locus_tag="RHV13_000462"
     CDS             complement(7080..8048)
                     /gene="hcr"
                     /locus_tag="RHV13_000462"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HDV1240023.1"
                     /translation="MTMPTNQCPWRMQIHHIKQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLASVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGNLVLA"
     gene            complement(8060..9712)
                     /gene="hcp"
                     /locus_tag="RHV13_000463"
                     /gene_synonym="priS"
     CDS             complement(8060..9712)
                     /gene="hcp"
                     /locus_tag="RHV13_000463"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HDV1240024.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(9856..10755)
                     /gene="lysO"
                     /locus_tag="RHV13_000464"
     CDS             complement(9856..10755)
                     /gene="lysO"
                     /locus_tag="RHV13_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HDV1240025.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQKAALRVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(11239..11934)
                     /gene="aqpZ"
                     /locus_tag="RHV13_000465"
     CDS             complement(11239..11934)
                     /gene="aqpZ"
                     /locus_tag="RHV13_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HDV1240026.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            12360..14018
                     /locus_tag="RHV13_000466"
     CDS             12360..14018
                     /locus_tag="RHV13_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HDV1240027.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(14015..14971)
                     /locus_tag="RHV13_000467"
     CDS             complement(14015..14971)
                     /locus_tag="RHV13_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HDV1240028.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            15122..16237
                     /gene="macA"
                     /locus_tag="RHV13_000468"
     CDS             15122..16237
                     /gene="macA"
                     /locus_tag="RHV13_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HDV1240029.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            16234..18180
                     /gene="macB"
                     /locus_tag="RHV13_000469"
     CDS             16234..18180
                     /gene="macB"
                     /locus_tag="RHV13_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HDV1240030.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEILD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(18253..18477)
                     /gene="cspD"
                     /locus_tag="RHV13_000470"
     CDS             complement(18253..18477)
                     /gene="cspD"
                     /locus_tag="RHV13_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HDV1240031.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            18800..19120
                     /gene="clpS"
                     /locus_tag="RHV13_000471"
     CDS             18800..19120
                     /gene="clpS"
                     /locus_tag="RHV13_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HDV1240032.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            19151..21427
                     /gene="clpA"
                     /locus_tag="RHV13_000472"
     CDS             19151..21427
                     /gene="clpA"
                     /locus_tag="RHV13_000472"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HDV1240033.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(21770..21857)
                     /locus_tag="RHV13_000473"
     tRNA            complement(21770..21857)
                     /locus_tag="RHV13_000473"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21821..21823),aa:Ser,seq:gga)
     gene            complement(22111..22329)
                     /gene="infA"
                     /locus_tag="RHV13_000474"
     CDS             complement(22111..22329)
                     /gene="infA"
                     /locus_tag="RHV13_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDV1240034.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(22614..23318)
                     /gene="aat"
                     /locus_tag="RHV13_000475"
     CDS             complement(22614..23318)
                     /gene="aat"
                     /locus_tag="RHV13_000475"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HDV1240035.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPINFWVPRCLFSPQE"
     gene            complement(23360..25081)
                     /gene="cydC"
                     /locus_tag="RHV13_000476"
     CDS             complement(23360..25081)
                     /gene="cydC"
                     /locus_tag="RHV13_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HDV1240036.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERVVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(25082..26848)
                     /gene="cydD"
                     /locus_tag="RHV13_000477"
     CDS             complement(25082..26848)
                     /gene="cydD"
                     /locus_tag="RHV13_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HDV1240037.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGETELASTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(26971..27936)
                     /gene="trxB"
                     /locus_tag="RHV13_000478"
     CDS             complement(26971..27936)
                     /gene="trxB"
                     /locus_tag="RHV13_000478"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDV1240038.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDSFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTHNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            28481..28975
                     /gene="lrp"
                     /locus_tag="RHV13_000479"
     CDS             28481..28975
                     /gene="lrp"
                     /locus_tag="RHV13_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HDV1240039.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            29110..33204
                     /gene="ftsK"
                     /locus_tag="RHV13_000480"
     CDS             29110..33204
                     /gene="ftsK"
                     /locus_tag="RHV13_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDV1240040.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLTEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVVAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYVQPAAQ
                     YNEPLQQPVQPQQPYYAPAAEQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPAPEQP
                     VAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEET
                     KPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEA
                     AAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVP
                     TRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQ
                     QRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSP
                     MKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQQQYQQPQQPVAPQQ
                     QYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQSAAPQPQYQQPQQPVAPQPQDTLLH
                     PLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKA
                     DVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLE
                     LPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGS
                     GKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANAL
                     RWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLK
                     KEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIK
                     ANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEV
                     HAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASIS
                     GVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            33359..33970
                     /gene="lolA"
                     /locus_tag="RHV13_000481"
     CDS             33359..33970
                     /gene="lolA"
                     /locus_tag="RHV13_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HDV1240041.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            33981..35324
                     /gene="rarA"
                     /locus_tag="RHV13_000482"
                     /gene_synonym="mgsA"
     CDS             33981..35324
                     /gene="rarA"
                     /locus_tag="RHV13_000482"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HDV1240042.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            35415..36707
                     /gene="serS"
                     /locus_tag="RHV13_000483"
     CDS             35415..36707
                     /gene="serS"
                     /locus_tag="RHV13_000483"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDV1240043.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            36946..39390
                     /gene="dmsA"
                     /locus_tag="RHV13_000484"
     CDS             36946..39390
                     /gene="dmsA"
                     /locus_tag="RHV13_000484"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HDV1240044.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPDGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGIHNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            39401..40018
                     /gene="dmsB"
                     /locus_tag="RHV13_000485"
     CDS             39401..40018
                     /gene="dmsB"
                     /locus_tag="RHV13_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HDV1240045.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            40020..40883
                     /gene="dmsC"
                     /locus_tag="RHV13_000486"
     CDS             40020..40883
                     /gene="dmsC"
                     /locus_tag="RHV13_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HDV1240046.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(40919..41545)
                     /locus_tag="RHV13_000487"
     CDS             complement(40919..41545)
                     /locus_tag="RHV13_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDV1240047.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            41860..43008
                     /locus_tag="RHV13_000488"
     CDS             41860..43008
                     /locus_tag="RHV13_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1240048.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            43218..44648
                     /locus_tag="RHV13_000489"
     CDS             43218..44648
                     /locus_tag="RHV13_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDV1240049.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(44649..45557)
                     /locus_tag="RHV13_000490"
     CDS             complement(44649..45557)
                     /locus_tag="RHV13_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV1240050.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWITERPGFQVYYPNRQY
                     MSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            45657..46247
                     /locus_tag="RHV13_000491"
     CDS             45657..46247
                     /locus_tag="RHV13_000491"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HDV1240051.1"
                     /translation="MQSERIYLVWAHPRHDSLTANIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTPEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLINMCSYLGIED
                     ADVTFLCNTVVFDGQELHASYYQSLLSQVRDMVDAL"
     gene            complement(46329..47069)
                     /gene="pflA"
                     /locus_tag="RHV13_000492"
     CDS             complement(46329..47069)
                     /gene="pflA"
                     /locus_tag="RHV13_000492"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HDV1240052.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(47261..49543)
                     /gene="pflB"
                     /locus_tag="RHV13_000493"
     CDS             complement(47261..49543)
                     /gene="pflB"
                     /locus_tag="RHV13_000493"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDV1240053.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(49598..50455)
                     /gene="focA"
                     /locus_tag="RHV13_000494"
     CDS             complement(49598..50455)
                     /gene="focA"
                     /locus_tag="RHV13_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HDV1240054.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT        12460 a        12580 c        13408 g        12120 t
ORIGIN      
        1 gaataacgtg aaggttgctg cggatgcttt tcttgaaata ctttattctc ttaaaaataa
       61 atattgtatt aaactattaa gcgctcagga tcgggcgttt atatacgaat ttgcaagaat
      121 gatcttcgct tatattaatg acaaaagcat tgagatctta ttactttctt gttttgcggc
      181 ggcggaccaa aaagcaatcc agcggtatat acctcaggcg caagatgggg aagactttag
      241 gaatcattta caatataagt tgactcttcc cactcattaa tgttgccggc acaacataat
      301 agggcaggta agcggtgctc cataatgaaa ccaatatccg gttacgaaac agataattgt
      361 acaggcttat gtagctgttt ttgacacaat gagatgattt cctaaactga tcaaaacctt
      421 ctttccaaca tggcatatca gtccgataat gctttagaga tcgaaggctt aacaaacaag
      481 gagatcgatc atgcaatttt ctacaacccc tactctggaa ggccagacca tcgttgaata
      541 ttgcggtgtg gtgaccggcg aagcgatttt aggtgccaat attttccgtg atttctttgc
      601 cggtatccgc gatatcgttg gcggacgttc cggtgcgtat gaaaaagaac tgcgtaaagc
      661 acgggagatc gcctttgagg aattaggctc ccaggcgcgg gcgctggggg ccgatgccgt
      721 agttggtatt gatatcgact acgaaacggt cgggcaaaac ggcagtatgc tgatggttag
      781 cgtcagcggt acggcggtga aaacgcgtcg atgagaagag tcttctggct ggtcgctgcc
      841 gctctgttac tggcagggtg tgcaggcgaa aaaggcattg tcgagaaaga gggataccag
      901 cttgataccc gacgccaggc gcaggcggcg tatccgcgca ttaaagtgct ggtgatccac
      961 tacaccgcag atgattttga tagctcgctg gcgacactga ccgataagca ggtcagctcg
     1021 cattatctgg tccctgcggt accaccgcga tacaacggta aaccgcgcat ctggcaactg
     1081 gtgccggaac aagaactggc ctggcatgcg gggattagcg cctggcgcgg ggcaacgcgc
     1141 cttaacgaca cctctattgg cattgagctg gaaaaccgtg gctggcaaaa atcggcgggg
     1201 gtgaaatatt ttgccccgtt tgaaccggca cagattcagg cgcttattcc gctggcgaaa
     1261 gatattattg cccgttatca catcaagccg gaaaacgtag tggcacatgc ggatattgcg
     1321 ccgcagcgca aagacgatcc ggggccatta tttccctggc agcaactggc gcagcagggg
     1381 attggtgcct ggccggatgc gcagcgggtg aacttttacc ttgccgggcg cgcgccgcac
     1441 acaccggttg ataccgcgtc attgctggag cttttggcgc gctacggtta tgacgttaaa
     1501 cctgatatga caccgcgcga gcagcggcgc gtgattatgg cgttccagat gcatttccgc
     1561 ccgacgttat acaacggcga agcggatgca gaaactcagg caattgccga agcgttgctg
     1621 gagaaatacg ggcaggatta gcgcggcagt tttccgtggt cgcgtagcca ggcggcagtt
     1681 ttctcgatac cttcatccag ggtgatgacc ggctgataac ctaactcttc ctgcgcacgc
     1741 gtaatatcca gcgtaaagtc aaaattcaac ttggagacgc cgtagtgggt cagtggcggc
     1801 tcttttgctg acttgcggcc taaacgctcc atgctgcggg cgatcatatc caacatcggg
     1861 taaggaaccg aacgaatacg acagtcaata ttcaactcgt cgatcagctt ctgcacgatg
     1921 ctgcgcagtg tgcgatgctc gccgttggta atgttgtaca cacgcccgga aggtagctta
     1981 tcgcaggctt cctggcttgc cagccacatg gcgtgcactg cattttcata gtaggtcata
     2041 tcgaccagcg cacttccgcc gtgcggtaac agaatgctgc cgtagtggtg catcatatgc
     2101 gccagacggg ggataaagac tttatcgtgc ggtccgaaca gactttgtgg gcgcagaata
     2161 gtaaagcgcg tttgtggatt cgcctgcgaa agcatattga tcacttcttc gctggctgct
     2221 ttgctgcggg caaactcgtt ggcgaagcgg tgagggcgaa aatcttcttt aatatcgcga
     2281 tggtggtgat aatcgaagta cagggagggg gaagagatat gaataaagtt acgcacaccc
     2341 caggcgacag cccattcacc caggcggcga gtggcgcgaa cgttagccag atcgaaagcc
     2401 tgttgtgtcc cccagggcga ggtaaagctg gagcagtgcc acagcgtatc aatgcccgcg
     2461 agcatcactt tagcttgtga tgagaccagc tcggtcagat ccgccggaac aaactctgcg
     2521 cccatttttt ccagcaattt acccattgcc tcgttgcgac cggtcgctcg cacgctgatg
     2581 cctttctggc ataaaaactc taccgcgttt cgacctaagc cgctggtggc gccggtaacc
     2641 agtaccttca tatcaatcca ctgttgttga gaaaataacg tgcgcattct tccgtgattt
     2701 cccccatgat gcaatgggaa acatgaaaga ataacgcagg ttttgtcgat taatctgtgc
     2761 tttgttctgc cagtttggcg attcgttttg ccattccgcg aaaaataaac agatgcgcgg
     2821 ggatcatcaa taaccagtaa aacagccccg gcataccgtg cggatgccag aaagcgcgga
     2881 catcgatagt acgatagtcg cctttatctt ccagggtaaa acacagtcgt cccagccccg
     2941 gcgctttcat gccaaataac aacgtaagtt gtttttccgg ttcaacgaca atgactttcc
     3001 agctatccac tgcatcgcca gtctgtaaat attcgcgctc cgggcggcct ttcgccagct
     3061 tatgaccgat cgcgcggtcc atcaacgccc gtgtctgcca caaaatattg ccaaagaaat
     3121 aacgctcttt accgccgatt tggttcacca cctgccataa agccgcaagg ctggcggacg
     3181 ttttaacggt aaaccccgcc tgtttggcga aataaccgta ctccggtcgc cagcgggcaa
     3241 aggcctgagc gtcgtagccc cagtcgctgg agttgaccag tttttcttcc tctttcaacg
     3301 tgctacgtac cgcgtcatcg aaagcgatca gccgttgtgg gatgagcgca cgtagcgcgg
     3361 agtcatccgc cagcagatcg tgtttcagcc cctgaatcaa cgccctggcg gtggtgggcg
     3421 gtacggaagt aatcacattg agaaaccaca ccgaaatcca gcgggtgggg agggggatgg
     3481 ggatcaacca gcggcgctta ccgctcaccg ccataaaatg ttcaaactgt tgctgataac
     3541 tgagcacctc tggtccggcg gcttcgaaga tgcggtgttc gttggctgga tggtctaaca
     3601 gcgccaccag atagtgcagc aagttttcca gcgcaatggg cgtggtgcgt gaacgtaccc
     3661 agcgtggcgg cgttaacact ggcaggttgt agaccatatc gcgcatgact tcgaacgctg
     3721 cggaacctgc gccaacgata attccggccc gcagttcggt cactggtaca ttcgcttcac
     3781 gcagaatgtc cgccgtagcc tgacgggcgc gcagatggtc cgactgctca tgtggcgggg
     3841 cctgtaacga actgagaaag attaattgct taactggtac ttcacgtagc gcatcgcgga
     3901 cgttgagagc cacctggcgc tcctgagcga taaaatcgcc gccttcgccc atgctgtgca
     3961 ccagaaaata gacggtatcg atatcctgca acagggccgg aaggttatcc ggccagctga
     4021 gatcgacttt atggcaactg acgtttgcca gttgcagctt tgcaagcctg tcgacatgac
     4081 gtgccgccgc caggatctga tgtccttgct ggctgagtgt gcgcaccaga tgctgaccaa
     4141 tgtagccact ggcaccgaga actaaaatgc gttgcggcac gtctctctcc ttaacgcgcc
     4201 aggaatgcgc gccagtgggc ggcgacttcc gccagttgtt cgcgggagac gtcaagatgc
     4261 gtcaccaggc ggacaatcgg cgaggcgtta atcagcacgt ttctcgcttt catgtattcg
     4321 cctaacgcgg cagcattttc ttccccgacg cgaacaaaca gcatattagt gtcctgacgc
     4381 atcacatccg cgcctgcttc acgcagctgc tccgccatcc aggcagcgtt gtcgtggtct
     4441 tcctgcaagc gcgcaacatt atttttcagg gcatatatcc cggcggcagc cagaatgccg
     4501 gactggcgca tcccgccacc tgtcattttc cgccagcgaa tggcacgttt aatgtaatca
     4561 cgattgccga cgagtaatga accgactggc gtcccaagac ctttcgacag gcaaatggtg
     4621 aacgaatcac aatattgcgt aatctctttc agttcgcagc cgtaagccac cacggcgtta
     4681 aagatgcgcg cgccgtcaac atgcagcgcc agatttcgct cgcgggtaaa ttcccatgct
     4741 tctttaaggt attcacgcgg cagcactttg ccgttgtggg tgttttccag actgagtaat
     4801 ttggtgcggg cgaaatggat atcgtcgggt ttgattttca tcgccacttt atccagcggt
     4861 agcgtgccgt cggcagccgc gtcgatgggt tgcggctgaa tactgcccag caccgctgcg
     4921 ccaccggctt caaacagata gttatgcgcg gcctgaccga caatatactc ttcgccgcgt
     4981 tcgcagtgac tgagcagagc gaccaggttg gcctgagtgc cggtaggcag aaaaatggcg
     5041 gcttctttac cggaaagctc tgccgcatag tcctgcagag cattaacggt agggtcgtct
     5101 ccgtaaacgt cgtccccaac cggggcggcc atcatcgctt cgagcatggc gcggctcggt
     5161 cgggttacgg tatcactgcg taaatcaatc atggcatgtc cttattatga cgggaaatgc
     5221 cacccttttt accttagcca gttcgttttc gccagttcga tcacttcatc gccgcgtccg
     5281 ctgatgattg cgcgcagcat atacaggctg aaacctttgg cctgttcgag tttgatctgc
     5341 ggtggaatgg ctaactcttc tttggcgacc accacatcca ccaacaccgg accgtcgatg
     5401 gagaaggcgc gttgcagggc ttcatcaact tcagacgctt tttctacacg gatacccgta
     5461 atgccgcatg cttcggcgat acgggcaaag ttggtgtcgt gcagctcagt accgtctgtc
     5521 aggtagcctc cggctttcat ctccatcgcc acaaagccca gcacgctgtt attaaagacg
     5581 ataattttca ccggcagttt catctgcact accgagagga aatcgcccat caacatgcta
     5641 aaaccgccat cgccgcacat ggcgaccacc tgacgttccg gctcggttgc ctgcgcgccc
     5701 agcgcctgcg gcatggcgtt agccatcgaa ccgtggttaa acgaacctaa caggcgacgc
     5761 ttgccgttca ttttcagata acgcgctgcc cacaccgttg gcgtaccaac gtcacaggtg
     5821 aaaatggcgt catcggcggc aaaatgacta atttgctgcg ccagatattg cgggtgaatg
     5881 gctttctcgc tcggtttagc taaatcgtcc agccctttgc gggcatcgcg gtaatcttcc
     5941 agcgctttat ccagaaactt gcgatcggtt ttttcttcca ccagtggcag caatgcacgc
     6001 agagtcgatt tgatatcgcc gaccagtgcc atatccacct tgctgtgagc gccgatgctg
     6061 gctggattga tatcaatctg aataattttg gcatcggtcg ggtagaaggc gcggtaggga
     6121 aattgcgtgc cgagtagcac taacgtgtcg gcgttcatca tggtatggaa acctgacgag
     6181 aatccgatta acccggtcat tccaacatca tacggattat cgtattcgac atgctcttta
     6241 ccgcgtaggg catgaacaat aggggcttta attttcccgg caaactcaac taactcttta
     6301 tgcgcccccg cgcagccgct gccacacatc agagcgatat tgctggaata acgcagcagt
     6361 tgcgccagtt tgcgtaactc ttcttcttcc ggcgtcacga ctggctgtgg cgcatgatac
     6421 cagtgcgtgg ttgccccttc tggcgcaggt tttaacgcca cgtcgcccgg taacacgaca
     6481 acggaaacgc cacggttaag caccgctttg cgcatagcaa ttgccagcac ttgtgggatc
     6541 tgctccgggc tggaaaccag ctcgcaatag tgactacatt cgcggaatag ctcttgtggg
     6601 tgggtttcct ggaaatagcc gctgccaatt tcgctggagg gaatatgagc ggcaatcgcc
     6661 agtaccggaa cgtgattgcg gtggcaatcg aacaggccgt tgattaagtg caggttgccg
     6721 gggccgcacg atccggcaca gaccgccagt tctcctgaaa gttgtgcttc agcgccagcg
     6781 gcaaacgccg ccacttcttc atggcgggtg gacatccact cgatggtgcc catgcgatta
     6841 agactgtcgc taagaccgtt cagagagtcg cccgtgactc cccagatgcg tttcacgcct
     6901 gccgattcga gtgttttggc gatataagct gcaaccgttt gtttcatggt tctccatctc
     6961 ctgaatgtga taacggtaac aagtttagtt catctgacgg agggggaagg gatgggagaa
     7021 aaaggaggca ctaacggtta aacagcccga tgacaggaat atcatcgggc ataaggcgat
     7081 tatgcgagaa ccaaattccc ctgcggatgg caggagcagg ccagtacgta accttcagcg
     7141 atttcggcgt cggtcagcgt cattgtgctg ctcaccgtat attcaccgga aaccactttc
     7201 gtcttacagc agccgcaaac gcccgcacgg caggcggcga caaccggaac gttattgctt
     7261 tccagcgcct ccagtagcgt ggtgcctacc ggggcgtaaa attctcgtgc cggttgcagt
     7321 ttggtgaatt tcagaccgct ggtcgccgct tccgctactg gggtgaagaa tttttcttta
     7381 aagaaacgcg tcacgccgag cgccttcact tcctgttcta cccaatccat atacggtgct
     7441 ggaccacaag tcatcacggt acgtgaggct aaatcaggaa cgcttgccag cagttcgcga
     7501 gtgagacgac cagcgataaa gccttcggta acgttatttt ccgccaccag cgttaccgga
     7561 tagttacgcc actcatcggc gaaaataaca tcctgcggcg tacgcacgtt gtagatcacc
     7621 cgcacatcgg cctgtggacg gttcttcgcc agccagcgac gcatcgacat aatcggcgtg
     7681 acgccgcagc ctgccgccag caacaggaat ttatcttctg ctttatcgtc gcaggtaaat
     7741 tcccccatcg cgtctgaaag ccagagataa tcaccgcgtt ttacatcgcg cgtcagccac
     7801 tgggagccga caccgtcatc aatccgccgc acggtcaggg tgatatattc actcacacct
     7861 ggcgtggagg aaatagtgta agcacgcagc gtttccgctg agttacgcac gctgaccagt
     7921 gcatattgcc cggcgcgata tgggtagtaa tcgtggcaaa tcagggaaat cgtccacaca
     7981 tccggcgttt cttgcttaat gtgatgaatc tgcatccgcc acgggcattg attcgttggc
     8041 atcgtcatct gctaactcct tacgcgctca acagttgctt catgtcttct tcaacagtgg
     8101 tgatagaacg caggccgaat ttctcgttca gcaccgccag cagatctggt gtcaggaaac
     8161 caggtgcagt cgggccggtg acgatatttt tcacgcccag agaaagcagc gtcagcagaa
     8221 tgacaatcgc tttctgttca aaccaggaga gcaccagcga cagcggcaga tcgttcacac
     8281 cacagcccag tttttctgcc agcgttaccg ccagaataat cgctgagtaa gcatcgttac
     8341 attgacctgc atctaccaga cgcggcagac cttcgatatc gccaaactca agtttgttaa
     8401 agcgatattt accacaggcg agggtcagga tcaggcagtc atccggcacg ctggtggcga
     8461 aatcggtgaa gtagtggcgc tcgccgcgtg cgccgtcaca gccaccaagc aggaagatat
     8521 gacgcagttt ttcacggctc accagatcaa tcagcgtatc cgctgcgcca agcagcgtct
     8581 ggcggccaaa gcccacggtg atcaggtgcg gaatttcgct gtacgggaag cctgccattt
     8641 gttgcgcctg ggcaatgacc gcagagaaat cttcgccatc cagatgacgc acgccaggcc
     8701 agccaacgat gctgcgggtc cagatacgat cgtcataagc gcctacggtt gggtcgatga
     8761 tgcagttcga ggtcatcacg atggggccag ggaaacgagc gaactccact tgctgattct
     8821 gccagccgct gccgtagtta ccgaccagat gcttgaattt acgcagctcc gggtagccat
     8881 gcgcaggcag catttcgccg tgggtgtaga cattaacgcc cgtgccttcg gtctgttcca
     8941 gcaggttgta gagatctttg agatcgtgac cggaaatcag gatgcattta cccgccgtcg
     9001 ctttgacgtt gacctgggtt ggcgtcgggt gaccgtattt accggtttcg cctgcatcca
     9061 gaatgctcat cactttgaag ttcatctggc cgatttccat tgaacactca agaagcgcgt
     9121 tcatatcggc aggccaggtc cccagccacg ccatgatttt atgatactgg gcataaatat
     9181 cgttgtcgta ttgaccgaga acgtgtgcgt gttccatata ggccgccgca cctttcaggc
     9241 catacaggca cagcagacgc aggccgagaa tgttttcgcc aatcgccgct ttatctttgt
     9301 taggggtaaa ttctgctgcc tgacgttgca gttcgccgag atcgtcgctc accagttgca
     9361 ggtcagccat cgggttatcg acgcgggcgt tggcatctac agccaggcat tgtgctttca
     9421 gcgcctcgcg caaggcaatc gcttcacgag cgtagccgac aatacgcgga gaatcgaagt
     9481 taacgttggt cagggttgag aaaaaggcgc gtggcgcgaa gctgtcgaca tcgtggttga
     9541 tgatgccgta ttcacgcgct tttaccgccc aggcagaaag cccttgcagc gccgcgatga
     9601 gtaaatcctg aaggtcagag gtttccgccg ttttaccaca catcccctgc gcgtatgagc
     9661 agccgtttcc tgccggagta cggatagttt gttcacattg cacacaaaac atgatcacac
     9721 cttttaaagt tatatttaat atacatgttt aaggttaaga cgcttaacgc ggggataaaa
     9781 gggatttttc atgcaacttt aagggagatt gatttagcgc aattttggcg gcagggatct
     9841 accgccagag aggtattacg cagagaaaaa ggcgatgagg atcggcacta acaggctaag
     9901 aataaaaccg tgaacaattg ccgccgggac catatccagc ccgccagtac gttgaagaac
     9961 gggcagggtg aaatccatcg acgtcgcacc gcataagccc agtgcggtag agcggctgcg
    10021 gcgaatcagc ccagggatca acataattgc aatcagctca cgggccagat cattaaaaaa
    10081 cgccgcgctc ccgattaccg gaccaaaaga ttcggtcaat aaaataccgg aaagagaata
    10141 ccagccgaaa ccagaggcca ttgccagcgc ggtattgatg ggtagatcaa gaataaaggc
    10201 gttaattaaa ccaccaatta atgaactggc aaccaccacc acggcgacaa tcattccccg
    10261 gcgattaagg acaatctgct ttaaggtcat gccattattg cgcaactgaa taccgacgag
    10321 gaaaagtagc agaattaagg tgtattcact ggcttcggtc gcgtgttgta agaaagccag
    10381 tccactcagg ccaatggcaa aaccaatcac tactacgccg cacagtttta gcgactcaag
    10441 cgccatcgca atacgcgacg ggagtttttc ttgctgatgg tggttgcgcc acggcagacc
    10501 gcgctccagc cacataaggg cggcaatatt acacagtaaa ataacggtaa tactgacggc
    10561 agaataatgc agaatcgcca acaggttact ggcgaggtta tcgagaaacg ccagactgat
    10621 acccataaaa aagagaataa ggtaaaccat ccagcttaat agctgattaa taactcttaa
    10681 tgcagctttt tggcgaagcg gaatgaggta acccacaatc aggggaacca gaatgattaa
    10741 cagcccagaa aacatgaaaa tccagtcctt gcaaagatga agtcgaaatg cgcgatgaca
    10801 cactactgaa agcggaagga cgagtaaagt tgcaattaaa aggaaatgtt atgcataagg
    10861 atcggtaggg tattcatttt catttaaaga tattcttgcg ctttaattac aaaccgcacc
    10921 ggtgttggtg gcgtcaaaat cgccgaggcg ttccctgaag gccggggcag cccacatgga
    10981 tgtgggctga gggcgcgttt tacagggatg ttacctcgcg cccgacccgg tagccgcaag
    11041 ggataagctg agggtaccgc gcagcggcga ttttgttcgc cagagccagg gggtgcaggg
    11101 ggcggcggcg attggccgcc ccctgcgcgc tccctgcgcc agcgactata tgtggcttaa
    11161 cacatcaagg agcgcaacag gaagatgaat caatatattt aaaaaaggcc tgacattacg
    11221 ccaggccttc tgcgttaatt aatcacgctt ttccagcaga gtccggtaaa tcagaccacc
    11281 gataatgcca ccgacaattg gcaccaccca gaagaaccac agttgttcta atgcccagcc
    11341 gccctggaag atggcaaccg cggtgctgcg cgccgggtta acagaagtgt tagtcaccgg
    11401 aatactgatt aagtgaatca gggttaaggc cagaccaata gcgatcggcg caaaacctgc
    11461 cggagcgaat ttgtcggttg caccgtggat cactaacagg aagcccgcac tcaataccag
    11521 ttcaactacc agcgcggaaa gcatggaata accgcctggt gaatgctcgc cataaccgtt
    11581 agaagcaaaa ccgctggctg ccgcgtcaaa acccgtttta ccactggcaa ttaaatacag
    11641 cagcgctgcc gcaacaatac cgccgacaac ctgggcaatt acgtagccaa cgacttcttt
    11701 tgccggaaaa cgtccgccag cccataaacc aatagtgacc gccgggttaa aatgaccacc
    11761 agaaatatga ccaacagcaa aggccatcgt cagaacggtc agaccgaacg ccaacgccac
    11821 gccggcaaaa ccaatgccta attccgggaa gcctgcggcc agtacagcac taccacagcc
    11881 accaaaaaca agccagaaag taccaaaaca ttcagctgct aattttctga acatatccac
    11941 ctcaattaaa aattgaccct gtgaaaaata tggtcgtttt atagggtcgt cgtaaaaaag
    12001 tgacgacgga aataatgcgc ggctatttta aaaacgaagg cgagtcattc accagataaa
    12061 taaatccagt aaatttgatt tagggcaaca gcgggttgcc ccatatagtc atttgtctga
    12121 ttgacagtgt agtgcaagca aaagatttaa tcctttaggc gtaataaaaa ataatttatc
    12181 atgctaatta tttgattttg ttgtttttgc agacttatca gcaagaggga gtataacgcg
    12241 attattcgct catttttcag acatttgcca tgcttaaatg tgatgtcatc acgtattagc
    12301 aaggcctttc ccgttatact gccagcgtaa aggataagtc acatatttct ggaggggata
    12361 tgattcttga gcgcgttgaa attgtgggtt ttcgcggtat caaccgtttg tcgttgatgc
    12421 tggaacaaaa caacgtcctg attggggaga acgcgtgggg taaatccagc ttgctggacg
    12481 ccttaaccct gctgctatcg ccagaatcag atctctacca ttttgagcgc gacgatttct
    12541 ggttcccgcc gggagatatc aacggtcggg aacatcatct gcatattatt ttgaccttcc
    12601 gcgaatcgct gccaggccga catcgggttc gccgttatcg gccgctggaa gcgtgctgga
    12661 cgccatgcac cgatggctat caccgtattt tttatcgtct ggaaggggag agtgcggaag
    12721 acggcagcgt gatgacactg cgcagttttc tcgataaaga cggacatccg attgatgttg
    12781 aggatattaa cgatcaggca cgccatctgg tgcgtttaat gccggtgctg cgcttgcgtg
    12841 atgcccgttt tatgcgccgt attcgtaacg gcacggtgcc aaatgtcccc aatgtggaag
    12901 tcaccgcgcg ccagctcgat ttcctcgccc gtgagttgtc ctcacatccg caaaatctct
    12961 ctgatggaca gattcgtcag ggactttccg caatggtgca gttgcttgag cattatttct
    13021 ctgagcaggg ggctggacag gcgcgatatc gtttaatgcg gcggcgggcc agcaatgagc
    13081 aacgaagctg gcgctatctg gacatcatca accggatgat tgaccgacct ggagggcgct
    13141 cgtatcgggt tattttgctc ggcctgtttg ctactttatt gcaggcaaaa ggcacattgc
    13201 gactggataa agacgcccgt ccattgttgc tgatcgaaga tccagaaacg cgtttacacc
    13261 ccattatgct ttcagttgcc tggcatctgt tgaatctttt gcctttacaa cgtattgcta
    13321 ccaccaactc tggcgagctg ctttcgttaa ctccagtgga acatgtttgc cgcctggtgc
    13381 gtgaatcttc tcgtgttgcc gcctggcgtc tggggccaag tggcttgagc accgaagaca
    13441 gccgccgcat ctcttttcac attcgtttta atcgtccgtc atcgcttttt gctcgctgct
    13501 ggttgctggt ggaaggggaa acggaaacct gggttatcaa tgaactggcg cgtcagtgcg
    13561 gacatcattt tgatgccgaa gggatcaagg tcattgagtt tgcccagtcc gggctaaagc
    13621 cactggttaa atttgcccgc cgaatgggga ttgaatggca tgtactggtc gatggcgatg
    13681 aagcagggaa gaaatatgcc gctacggtac gcagcctgtt gaataatgat cgggaagccg
    13741 aacgagaaca tttaacggcg ttaccggcgc tggatatgga acattttatg tatcgccagg
    13801 gattttccga tgtgttccac cgcgtggcgc aaatcccgga aaatgtaccg atgaatctgc
    13861 gcaaaattat ctcgaaagcg atccatcgct cttccaaacc cgatcttgcc attgaagtgg
    13921 caatggaggc cggacgtcgt ggtgtggact ccgtaccgac gctgctgaaa aaaatgttct
    13981 cacgcgtgct gtggctggcg cgcggtcgcg cggattaacc gcgaaacatc gtggccattt
    14041 gtggctgaat agcgtcgagc atctcatagc gccgacggta ttcagcccgt tttttactgg
    14101 cgatttcggc aatctctttt cgtgctatct gtgctggaag gcggtaatgg cgttcagcat
    14161 cacatacgcc gccaaccgat tcccagaaag cgttgtaatc agcgtggatc ttgccttctt
    14221 tatcgcgata acgcaggctg cggtaaatat gcgtttcatt gctgacggca ataatctgct
    14281 ctacctgcaa acgttgggca aacagacagg ccgcttccat cacgaggcgt ttgggaaata
    14341 gcccgtggca ggctttcgtc gcattctgga tttcctgatg tggaatttcc cattttgcgc
    14401 cttgcagtcc gccaataaac atcgttcttt tcccctgata ttcacacagg gtaaacgtga
    14461 tctctgccag aggaatacct tcgctgttgc gaaacaggat tgtgctgtca ccttctttat
    14521 ccattgagat catcatggtc agctcaagcg tgaactgctc gccgtttttg ccttccagct
    14581 tcgccagttg cagcccgggg gtattcaaat ataagctgaa ttcttccgcc gacatacatc
    14641 cgcggagtaa cgcataatgg taacgtaacg cctccagcaa ttgcttacgg ctaagattcg
    14701 ccgcaaggta agggcgatgc agacgcacag gcagtcgcgg ttggcgcgtt aacaacacat
    14761 tgagattagg ccagtgggaa agttcgttca tccactcaac gcttaaacgc ggcataatca
    14821 acgagcgcag caaaaatttc tggcgaaaac tacggcgatg ccagaattta cccggccgac
    14881 actgtccacg tgccagacta agaaaaagtg acaggctgct gagagattca gatggcgtaa
    14941 aggtccgttc agttagctgc gacatattca tgaaatcaat ggttatacat gacgtcgatt
    15001 tcaccattgc gtatcttaac caaacatcaa tagtgtgatt actaacgtaa attttatggt
    15061 tttgttgata tttcgttgaa gttaatgacc cggattggca tatggagtat tcagaatatt
    15121 tatgaaaaag cggaaaaccg tgaagaagcg ttacgttatt gcgctggtga tagtcatcgc
    15181 cggactgatt acgttatgga gaattcttaa cgcacccgtg ccgacttatc agacactgat
    15241 tgtgcgcccc ggtgatttac agcaaagcgt gctggcgacc ggaaagctgg acgcgctgcg
    15301 taaggttgac gtgggcgcgc aggtcagcgg tcagttgaaa acgctgtcgg tggcgattgg
    15361 cgataaagta aaaaaagacc agcttttagg ggtcattgat cctgaacagg ctgaaaacca
    15421 gatcaaggag gtcgaagcaa cgctgatgga gctacgcgcg cagcggcagc aggcggaagc
    15481 ggagctgaaa ctggcgcggg tgacgtattc ccgtcagcaa cgtctggcac aaacgcaggc
    15541 ggtttcacag caggatctcg acaccgccgc gacggagatg gctgtgaaac aggcgcaaat
    15601 tggcaccatt gacgcgcaaa tcaagcgcaa tcaggcttct ctcgatacgg ctaaaaccaa
    15661 tctcgattac actcgcatcg ttgccccgat ggccggggaa gtcacgcaaa tcaccactct
    15721 gcaaggccag acggtgattg ccgcacaaca agcaccgaac attctgacgc tggcagatat
    15781 gagcaccatg ctggtaaaag cgcaggtttc tgaagcggat gtaatccacc tgaagccggg
    15841 gcaaaaagcc tggtttacgg tacttggcga tccactgacg cgctacgagg ggcaaatcaa
    15901 ggatgtacta ccgacgccgg aaaaggttaa cgacgctatt ttctattacg cccgttttga
    15961 agtccccaac cccaatggtt tgctgcggct ggatatgact gcgcaagtgc atattcagct
    16021 caccgatgtg aaaaatgtgc tgacgatccc tctgtcggcg ttaggcgatc cggttggcga
    16081 taatcgttat aaagtcaaat tgttgcgtaa tggtgaaaca cgcgagcgtg aagtgacgat
    16141 tggcgcacgt aacgataccg atgttgagat tgtcaaaggg ctggaagcgg gcgatgaagt
    16201 ggtgattggt gaggccaaac caggagctgc gcaatgacac ccttgctcga attaaaggat
    16261 attcgtcgca gctatcctgc cggtgatgag caggttgagg tgctgaaggg catcaccctc
    16321 gatatttatg ccggtgagat ggtagcgatt gttggcgctt cgggttccgg taaatcgacc
    16381 ctgatgaata ttctcggctg tctggataag gccaccagcg gcacctatcg cgtcgccggt
    16441 caggatgttg ccacgctgga cgccgatgcg ctggcgcaac tgcgccgcga gcatttcggc
    16501 tttattttcc agcgttacca tttgctttcg catttaaccg ccgagcagaa cgttgaagta
    16561 cccgccgtct atgccggtct tgagcggaaa cagcggctgc ttcgcgccca ggagttgctg
    16621 caacggctgg ggctggaaga ccgtacagag tattatccgg cacagctttc gggtggtcag
    16681 caacagcgcg tcagcatcgc gcgggcattg atgaacggcg gtcaggtaat tcttgccgat
    16741 gaaccaaccg gcgcactgga cagccattct ggcgaagagg tgatggcgat cctgcatcag
    16801 ctgcgcgatc gcgggcatac ggtgattatc gtcacccacg atccgcaggt cgctgctcag
    16861 gccgagcggg tgatcgaaat tctcgacggc gaaattgtgc gcaatcctcc cgccattgaa
    16921 aaagtgaatg ttgctggcgg gacggaacct gtggtcaaca cggtgtctgg ctggcggcag
    16981 tttgtcagcg gttttaacga ggcgctgacg atggcatggc gggcgctggc agcgaataaa
    17041 atgcgtactt tactgaccat gctggggatt attatcggta ttgcgtcggt ggtttccatt
    17101 gtcgtggtgg gtgacgccgc caaacaaatg gtgctggcgg atattcgttc tattggtacg
    17161 aatactattg acgtctaccc cgggaaagat tttggcgatg acgatccgca atatcagcag
    17221 gcgctgaagt acgacgactt aatcgccatc caaaaacaac cgtgggtcgc ctcagccaca
    17281 cctgccgtct cgcaaaacct gcgcctgcgt tataacaatg ttgatgttgc tgccagtgcc
    17341 aatggcgtga gcggcgatta ttttaatgtc tatggcatga ccttcagtga aggaaacacc
    17401 tttaaccagg aacagctgaa cggtcgtgcg caggtcgtgg ttctcgacag taatactcgc
    17461 cgccagcttt tcccccataa agcagatgtg gttggcgagg tgattctggt cggcaatatg
    17521 cccgccagag tcattggtgt ggcggaagaa aaacagtcga tgtttggtag cagtaaagtg
    17581 ctgcgtgtct ggctacctta cagcacgatg tccgggcgag ttatgggcca gtcgtggctg
    17641 aactccatta ctgtcagggt gaaagaagga tttgacagcg ccgaggcgga acagcaactc
    17701 acgcgtttac tttcactgcg ccacggtaag aaggatttct ttacctggaa catggacggc
    17761 gtcttgaaaa ccgttgaaaa gaccacacgt actttacaac tgtttctgac gctggtggcg
    17821 gtgatttcgc tggtagtggg cggtattggt gtaatgaata ttatgctggt gtcagtgacc
    17881 gagcggacgc gggaaattgg cattcgcatg gctgtaggtg cgcgagcaag cgatgttttg
    17941 caacagttcc tgatcgaagc cgtactggtt tgcctggtcg gtggcgcgtt gggaataaca
    18001 ctgtcactgt taattgcttt caccttgcag cttttcttgc ccggctggga gattggtttt
    18061 tcaccgttgg cactgctgct ggcgtttctc tgctcgacgg ccaccgggat tttatttggc
    18121 tggttacccg cacgaaatgc ggcacgactg gatccagtag atgctctggc acgagagtaa
    18181 tttttgagat aaaaatgcca gccgatcggg ctggcatttt gcctttagga tgtacacaat
    18241 gagacagaag agctatgcga ctgccgcttc tacttcgacg ggcacaataa cactggcgtg
    18301 attgcctttt ggcccctggt ggacatcaaa ctgaacggat tgtccagctt ttagcgttct
    18361 gtaaccatcc atctgaatgg tggaataatg agcgaaaata tcttcgccgc cgccttcagg
    18421 gcagatgaaa ccaaaccctt tggcattgtt gaaccactta acagtaccct tttccatgct
    18481 tcgacatcct tcgcaaatct tatacaagta agatggaata aaccggggtc agagaggggg
    18541 ctgttcaaaa cctcgccaac tctagaaata caatttagag aattagggcg agccgtcaag
    18601 cattagacag gggccagggg gcaggtatga atcaaaaatt tgaagcagtt aacgctattg
    18661 acaggaatgt gacagatgtc gctgatgcca acgatagatg atagttatct atcatgtgga
    18721 gtagattggt caggcaaata agctcttgtc agcggcaggg cgttctgccg ataaccgtaa
    18781 ccgaagatga taactgacaa tgggtaaaac gaacgactgg ctggactttg atcaactggc
    18841 ggaagaaaaa gttcgcgacg cgctaaaacc gccatctatg tataaagtga tattagtcaa
    18901 tgatgattac actccgatgg agtttgttat tgacgtgtta caaaaattct tttcttatga
    18961 tgtagaacgt gcaacgcaat tgatgctcgc tgttcactac caggggaagg ccatttgcgg
    19021 agtctttacc gccgaggttg cagaaaccaa agtggcgatg gtgaacaagt acgcgaggga
    19081 gaatgagcat ccattgctgt gtacgctaga aaaagcctga atgcaggcat aaaaattggg
    19141 ggaggtgcct atgctcaatc aagaactgga actcagttta aatatggctt tcgccagagc
    19201 gcgcgagcac cgtcatgagt ttatgaccgt cgagcacttg ttactggcgc tgctcagtaa
    19261 cccatctgcc cgggaggcgc tggaagcgtg ttctgtggat ttggttgcgc tccgtcagga
    19321 actggaagcc tttattgaac aaaccacacc cgttctgcct gccagtgaag aggagcgcga
    19381 cacacagccg acgctgagtt ttcagcgtgt actgcaacgt gcggtcttcc atgtccagtc
    19441 ctccggtcgc aatgaggtta ccggtgcaaa cgttctggtc gctatcttta gcgaacagga
    19501 gtcgcaggcg gcatatctgt tgcgtaaaca tgaagtcagc cgtctcgatg tggtgaactt
    19561 tatctctcat ggcacgcgta aagacgagcc gacacagtct tctgatcctg gcagccagcc
    19621 aaacagcgaa gaacaagctg gtggggagga acgtatggag aatttcacga cgaacctgaa
    19681 ccagcttgcg cgtgtgggcg gaatcgaccc actaattggt cgtgagaaag agctggagcg
    19741 tgctattcag gttctctgcc gtcgccgtaa aaacaacccg ctgctggtgg gggaatctgg
    19801 tgtcggtaaa accgcgattg cagaaggtct tgcctggcga attgttcagg gcgatgtgcc
    19861 ggaagtgatg gctgactgta cgatttactc tctcgatatc ggttctctgt tagcgggcac
    19921 aaaatatcgc ggcgactttg aaaaacgttt taaagcgttg ctcaagcagc tggagcagga
    19981 cactaacagc atcctgttta ttgatgagat ccacaccatt atcggtgcgg gggcagcgtc
    20041 tggtggtcag gtcgatgcgg ctaacctaat caaaccgttg ctctccagcg gtaaaattcg
    20101 tgtaattggt tcgacaacct atcaggagtt cagcaacatt ttcgagaaag accgtgctct
    20161 ggcgcgtcgc ttccagaaaa ttgatattac tgaaccgtca atcgaagaaa ctgttcaaat
    20221 catcaatggc ctgaaaccga agtatgaagc gcaccacgac gtgcgttata ccgcaaaagc
    20281 ggtgcgtgcg gcggtagagc tggcggtgaa atacatcaat gatcgtcatc tgccggataa
    20341 agcgattgat gttatcgacg aagcgggcgc tcgcgcacgc ctgatgccgg taagcaaacg
    20401 caagaaaacc gttaatgtgg cggatattga gtccgtggtg gcccgtattg cacgcattcc
    20461 agagaagagt gtttctcaga gtgaccgcga taccctgaaa aacctcggcg atcgcctgaa
    20521 aatgctggtc ttcggtcagg ataaagccat tgaggcgctg actgaagcca ttaagatggc
    20581 gcgtgcaggt ttaggtcacg aacataagcc ggtcggttcg ttcctgtttg ccggccctac
    20641 tggggtcggg aaaacagagg tgacggtaca gctttcgaaa gctttgggca ttgagcttct
    20701 gcgctttgat atgtccgagt atatggaacg ccataccgtc agccgtctga ttggtgcgcc
    20761 tccaggatac gttggttttg atcagggcgg tttgctgact gatgcggtca tcaagcatcc
    20821 acatgcggtg ctgctgctgg acgaaatcga gaaagcgcac ccggacgtgt tcaatattct
    20881 gttgcaggtg atggacaacg gtacgctgac cgataacaac ggacgcaaag cggacttccg
    20941 taacgtggtg ctggtgatga ccaccaacgc cggggtacgg gaaactgagc gcaaatccat
    21001 tggtcttatc caccaggata acagcaccga tgcgatggaa gagatcaaga agatctttac
    21061 accggaattc cgtaaccgtc tcgacaatat tatctggttc gatcatctgt caaccgacgt
    21121 gatccatcag gtggtggata aattcatcgt cgagttgcag gttcagctgg atcagaaagg
    21181 tgtttctctg gaagtgagcc aggaagcgcg taactggctg gccgagaaag gttacgaccg
    21241 ggcaatgggc gctcgtccga tggcgcgtgt catccaggac aacctgaaaa aaccgctcgc
    21301 caacgaactg ctgtttggtt cgctggtgga cggcggtcag gtcaccgtcg cgctggataa
    21361 agagaaaaat gagctgactt acggattcca gagtgcacaa aagcacaagg cggaagcagc
    21421 gcattaatct gattgtcagg taggttggtg aagtccgtaa tctcgtcagg ggttacggac
    21481 tttttattta tggggtggaa cggtgaaaac cctatttttg gaggtgaagg taagttgttg
    21541 ataattagtg ctgctggaag gtaaggataa aaaagggtgc tgcaggagaa tgggatggtt
    21601 ttgctttatt aacaacgggc taaacgtgta gtatttgagt tcactgccgt acaggcagct
    21661 tagaaattca caggtaacat actccacctg cccaccatgt tcactgccgt acagacagat
    21721 aaaatgcgaa aaaaaagctc gcactttcgt acgagctctt ctttaaatat ggcggtgagg
    21781 gggggattcg aacccccgat acgttgccgt atacacactt tccaggcgtg ctccttcagc
    21841 cactcggaca cctcaccaaa ttgttttgct gccaaacctc atgggtggca acggggcgct
    21901 actataggga gttggagtaa aacggtcaag aagaatttta atgataatta ttgtttgctc
    21961 atactgtaaa caagttgtgc agtatatcta catcgagaca agttatggac ttatacttcc
    22021 aaagtacttc atacatatca caaaataaaa aggccggtta aaccgacctt ttactcgttc
    22081 tttctcttcg cccatcaggc ggtaaaacaa tcagcgacta cggaagacaa tgcggccttt
    22141 gctcaggtcg tacggggtca gttcaacagt cactttgtcg cccgtcagga tgcggatgta
    22201 gtttttgcgc attttaccgg agatgtgtgc agtaaccacg tgaccgtttt ctaactctac
    22261 gcggaacatg gtattaggca acgtttcaag aacggtacct tgcatttcaa tattgtcttc
    22321 tttggccatc taatcctctg gggtatcact accgtaattt gaaccggcaa gataatgccg
    22381 aagttctgta aataagtaaa gatttgcgcg ctaaatcgca acaaacaggt tcggcacatt
    22441 accccgaaaa cacacggcta agccgcacca aaagcgcaac gtataaggga gcggtgagat
    22501 aaacgatggg cgttacctga cgcgaaaaat tccttatcgg cagcggggta atgagcgtaa
    22561 ccaactctgc gaccgcaatt ataacactct ggggagaaat gtgccgaaaa cattcattct
    22621 tgtggtgaaa acaagcatcg tggtacccag aaattaatcg gcaatcgtcc gaggcgcatt
    22681 tgattgagat aattaaggta atcccggcgg ggaatttcgc aggcaccaag cgatgctgtg
    22741 tgatcgttaa ggacctggca gtcgataagc ttaccgccat gaccgataaa ttcctcacag
    22801 aataccagaa gcgccgtttt agacgcattt tccatccggc tgaacatgga ctcgccacaa
    22861 aatagtgttc cctgggccac gccgtacata ccgccgacaa gctcatcttc acgccagact
    22921 tcaatggagt gggcatgacc gagttcgtga aggcgatggt aggcttcgac cacgccacgc
    22981 gtgatccagg ttccttcttc gcgatcgctg gcacagcctt caatgacctg accaaaagcg
    23041 taattcatcg tgacacgata gggcgagcgt ttatgaaatc gcttcatact acggctgata
    23101 tgcagtgatt ctggccatag caccgcgcgg ggatcgggcg accaccagag gatggggtcg
    23161 cctggagaaa accacggaaa aataccacgc tggtaagcca ttaacaggcg cgcagggcta
    23221 agatcgcccc caagtgccag caggccgtta ggctcacgta atgcgccttc cggggaaggg
    23281 aaggctattg aatggcgaga aagctgaacc aggcgcatga ccgcaaaact ccacgcaagt
    23341 cggatcgttc aataatagct tacaaaccct gcttgaactg gtaataacgc ccctgtctgg
    23401 caagcagttc tgcgtgagta ccttgctcaa taatttgccc gttgtccatc actattattt
    23461 gttggaaacg agagagtccg cgaagtcgat gggtgaccat taacaccgtt ttctcacgca
    23521 tcatttctgc aagcaattca aggatctggc tttcggttgt ggcatctaag ccttcggtag
    23581 gttcatccag caacaccagt ggcgcatcat gtaacagcgc acgggcgata gccagacggc
    23641 gcagttcacc accggagagc tggcgtccgc cttcacctaa ccaactgttg agacctgcat
    23701 cctcgagcag cttctccaga ccaacgcgac gcaagatctc cgacagagcc tcatcactac
    23761 tgccaggcga ggcgagtaac agattatcac gcagcgtggc gctaaacaga tgcactcgct
    23821 gaggaacaac gctgatggtc tgtcgtagag ccgcttcatt caggctggct atggggctat
    23881 cgttaagcaa aatctcgccc tgttgcgggt cccaagcgcg ggtcagcagt tgtaacagtg
    23941 ttgatttgcc gcatccggtt cgcccgagaa tcgcaatatg ttccccggcg tttacctgaa
    24001 gagaaatccc tttaagtgcc tgttgggatt gctccggata agtgaactga acatcccgta
    24061 atgtcagcga aacgcgatcg gcaacacgag tttgggtatc agggaaggtg acttccggtt
    24121 tttgatccgt taaatcagtg atccgaacgg cagaggcaat gacttgcccc agatgctgaa
    24181 atgcacccgt tactggtgcc agtgcttcaa acgcggctaa cgcgcagaag acaaacaggg
    24241 caattaacgc gccgggttga gcattgccgc caacgccgcc agacgccatc cacagcatca
    24301 ggatcaccgc taacgcgcca atgagcagca ttatcgcttg cgacaatgcg gtcagttcag
    24361 attgacggcg ttgcgcttcc agccattgaa tttctgtatt ctctagttgc gtgcgataac
    24421 gatcgctggc accaaaaatg gtcagctcag cttgcccttg cagccaggcc gtcagttgtt
    24481 ggcgatactg tccgcgaaga tgagtcagat tttgcccggt gctttttccc gcacgataaa
    24541 acagcggtgg catcaggaaa agcgtcagta acataatgcc gcccagcgta aaggcgaggg
    24601 tgaaatcaag gaaacttaac ccgattgtca ccaccataat caccacaaaa gcgcccacca
    24661 gcggcgagat aacgcgcagg taaagatgat cgagcgtatc aacatccgcc accacgcgat
    24721 tgagcaattc gccctgacga tagcgcgcca gtccggcagg ggagaggggc agcaatttgc
    24781 tgaaggtgta aatgcgcaga tgctgcaaca cgcggaaagt cgcgtcgtga cttaccacac
    24841 gttcaaaata gcgcccggca gtacgggtga ttgctgcgcc acgcacgccc gcagcgggta
    24901 gcatatagtt gaagctgtac agtccggcaa cccccgcaac cgctgaggcc gagaggaacc
    24961 agccggaaag tgtcaacaga ccgatactgg cgagcagcgt cacaattgcc agcacaatac
    25021 caagacttaa catccattta tgacgtttat acagtgccag atagggtagc aaagcgcgca
    25081 tttaaatctc ctcctgacga tgggccagta atgtggcgaa tgggccacca gccacactta
    25141 attccgcgta acgtccttgc tcaataatcc ggccatcctg cataacccaa atgacatccc
    25201 agtcagcaag atcttctaac tggtgggtga ccattaacgt tgtctggcgc agagaggcgg
    25261 cattcagcgc ctccattacg cgctgttcac tgtgagcatc aaggctggca gcgggttcat
    25321 ccaacagtaa tagcgaacag ggatttagta acgcacgggc caccgccacg cgctgcgcct
    25381 gccccacgga aaggcgggca gcctggtcgc caacaggcgt atcaacgcct tgtggcagga
    25441 gcggtagaaa ctcgctgacc caggcgttat ccagcgctgc ttgtaattct tgttcgctgg
    25501 catcaggtcg cgccagtagt acgttatccc gtaatgttgc tgccggtaat tgtgggtttt
    25561 gcccaaccca ggagagatgt ttacgccatg attctggtga taaatcgcgt aattctatcc
    25621 cgttgattcg taatgatccc tgatatgaga gaaaaccaga aagcgcgttc agcagtgagc
    25681 ttttacctga accgctgcga ccaaccaaca cggcacgttg gcctgctggc aaagtaaagt
    25741 tcagcggtcc ggccagcgtt ttaccttccg gcgacgtgat aaacagatcc tcggcctcaa
    25801 tggtcagcgg atcggttgat gctaattccg tctcaccgcg ctgcggatgg gcgagcgggg
    25861 tttccataaa cgttttcaga ctgtcagctg caccaacagc ctgggcttta gcatgataaa
    25921 acgtaccgag atcgcgtaat ggctggaaaa actctggcgc aaggataagg gccagaaaac
    25981 ccgcagccag cgtcacgcca gtatcgtagt gaccaaaatc cagctcgccg agataggaaa
    26041 aaccaaagta gaccgccacc agagcaattg acagcgaggt aaaaaattcg agaatgccgg
    26101 aggataaaaa tgccagccgt agcacttcca ttgtccgttg gcggaaatct tccgaagcag
    26161 aacgaatact ttcaatttca gcttcaccac gaccaaaaat acgcaatgtt tccatgccgc
    26221 gcagacgatc gaggaaatgc ccacttaagc gagcaagagc gagaaagtta cgtcggttag
    26281 catcagcagc ccccattcca accagcgcca taaacaacgg aattaacggt gcagtgccca
    26341 gcagaatgag cgccgcagcc cagtttgagg ggaagattgc caccacaatc agcaacggca
    26401 ccgacaccgc cagcgccatt tgcggcagat agcgtgcata gtaatcatgc atatcgtcaa
    26461 tttgctcgag aaccaacgtt gcccagctcc ccgcaggttt accctgaatc cacgctggcc
    26521 ctgcttgttg cagacggtcg agaacctgac ggcggatggc aaagcggata tgctgcccgg
    26581 cgtgataacc cactcgttca cgtaaccaga ccacccatgc gcgcagtaca aaggtcagaa
    26641 ccagtaacgt aaagggaagc agcagggctt cacggggaat attctccata atcatatgtt
    26701 gaagaatacg cgccatgaac caggcctggg caatgatcaa tatgccgctt acaaagccca
    26761 gcagacgaga aatattcagc caacgttggg agatgacgct ttgctgtttt aaccagcggg
    26821 ttaactcttt ttgacgagat ttattcattg cacgcttagc aggtgagtta tcagaattat
    26881 ttgcagagca atgttacaac ggggaaaaaa taaaggcgac ccatagtcgc aaggtgtcgc
    26941 cttctttact tttgttactg atttgtaaaa ttattttgcg tcagctaaac catcgaggta
    27001 gcgttccgca tcaagtgctg ccatgcagcc tgtaccggcc gaagtaatgg cctggcgata
    27061 aatgtgatcc atcacgtcgc ctgcggcaaa gacgccagga atgctggtct gggtggcatt
    27121 accatgaata ccagactgta ctttgatgta gccgttttcc agttccagct gcccttcgaa
    27181 aatcgcagta ttcgggctgt gaccgatagc aacaaacaga ccggcaacgt cgagtgactc
    27241 gatgttatcg ctgttgtgcg tatcgcgcag acgaacgcca gtgacaccca tttgatcgcc
    27301 ggtcacttct tccagcgtac ggttggtgtg cagaatgatg ttgccgttct ccactttatc
    27361 catcagacgc ttaataagga ttttttccgc gcggaaactg tcacggcggt gaatcagatg
    27421 cacttccgaa gcgatgttag acagatacag cgcctcttca accgcggtat tgccgccgcc
    27481 gatgaccgca actttctggt tgcgatagaa gaaaccgtcg caggttgcac aagcagaaac
    27541 cccacggcct ttaaaggctt cttcagaggg caggccgaga tagcgtgcag aagctccggt
    27601 ggcaataatc agcgcgtcgc aagtgtattc gccgttatcg ccattcagac ggaacggacg
    27661 gttttgcaga tccaccttgt tgatatgatc aaaaatgatc tcagtttcaa acttggtggc
    27721 atgttcgtgc atgcgctcca ttaataacgg accggtcaga tcgtttggat cgccaggcca
    27781 gttttccact tccgtggtgg tggtcagttg gccgcctttt tccatgccgg taatcagcac
    27841 aggttgcagg ttggcgcgcg ccgcgtagac agcagcggtg tatcccgccg ggcctgaacc
    27901 caggataagc agtttactgt gtttggtcgt gcccatgaga tccccatagt tgttggcaga
    27961 caatgggcag gattgtaggg aatttacaga cgtaaaaaaa gagtatgacg attttgttaa
    28021 caatttgtgc aatcggcagc atcgataagc aggtcaaatt ctcccgtcat tatcacctct
    28081 gctacttaaa tttcccgctt tataagccga ttaaatgatg aataaacgcc cctgttaatg
    28141 aatatctggc atgttgtact aaaaatcgat gttttgcttt gacaatcccc tggtgttttg
    28201 cgaaaacatt cgaggaagaa aaaaaacagt attcttatat gcgcataacc atgcatgtaa
    28261 ataccatgtt taccgtgcta gtgaaatcta cgtatggcgt ggacagacgc cattcgtgat
    28321 gtcgatagct gccacaaggc aacggtcttc tcaccgtaga cccaggcatt gcgcgccgtg
    28381 aatcttcatg atttcggtct atcgtgacgg gtagcgactc tgaacagtga tgtttcaggg
    28441 tcagacagga gtagggaagg aatacagaga gacaataata atggtagata gcaagaagcg
    28501 ccctggcaaa gatctcgacc gtatcgatcg taacattctt aatgagttgc aaaaggatgg
    28561 gcgtatttct aacgtcgagc tttctaaacg tgtgggactt tccccaacgc cgtgccttga
    28621 gcgtgtgcgt cggctggaaa gacaagggtt tattcagggc tatacggcgc tgcttaaccc
    28681 ccattatctg gatgcatcac ttctggtatt cgttgagatt actctgaatc gtggcgcacc
    28741 ggatgtgttt gaacaattca ataccgctgt acaaaaactt gaagaaattc aggagtgtca
    28801 tttagtatct ggtgatttcg actacctgtt gaaaacacgc gtgccggata tgtcagccta
    28861 ccgtaagttg ctgggggaaa ccctgctgcg tctgcctggc gtcaatgaca cacggacata
    28921 cgttgttatg gaagaagtca agcagagtaa tcgtctggtt attaagacgc gctaacacgg
    28981 aacaggtgca aaatcggcgt attttgatta cactcctgtt aatccataca gcaacagtac
    29041 tggggtaacc tggtactgtt gtccgtttta gcatcgggca ggaaaagcct gtaacctgga
    29101 gagcctttct tgagccagga atacactgaa gacaaagaag tcacattgac aaagttaagt
    29161 agcggccgtc gccttctgga agcgttgctg atccttattg tcctgtttgc cgtctggttg
    29221 atggctgcct tactaagctt taacccttcg gaccccagct ggtcgcaaac ggcctggcat
    29281 gaacctatcc ataatttagg tgggatgccc ggtgcgtggc tggcagatac gctgttcttt
    29341 atttttggcg tgatggctta caccattccc gtcattattg tcggcggttg ttggtttgcc
    29401 tggcgtcatc agtccagcga cgaatacatt gattattttg ccgtttcgct acgcatcatt
    29461 ggcgttttgg cgctcatcct tacctcctgt ggtctggcgg caatcaacgc tgacgatatc
    29521 tggtattttg cctccggtgg tgtcattggc agcttactaa gtactacgct acaaccactg
    29581 ctacacagta gcgggggaac tattgcgctg ctctgcgttt gggctgcggg cctgacgctg
    29641 ttcaccggtt ggtcatgggt gaccattgct gaaaaactcg gcggctggat tttaaacatt
    29701 ctcaccttcg ccagtaatcg tacccgtcgc gatgatacct gggtcgatga agatgagtat
    29761 gaagacgacg aagagtatga agatgaaaat cacggcaaac agcatgaatc acgccgtgcc
    29821 cgtattcttc gcggcgcgct agcgcgtcgt aaacggttga cggaaaaatt cattaatccg
    29881 atggggcggc aaacagacgc tgcgttgttc tccggtaagc ggatggatga tgacgaagag
    29941 attacctaca ctgcacgcgg tgtggctgcc gacccggacg acgtcctatt ttcgggcaat
    30001 cgtgcaacgc agccagaata tgacgaatac gatccattat taaacggtgc gccaattacc
    30061 gaacctgtcg ctgtagtagc tgctgctacc acggcgacac aaagctgggc tgcgccggtt
    30121 gaacctgtga ctcagacgcc gcctgttgcc tctgttgatg ttccaccttc gcaacctaca
    30181 gtagcctggc agcctgtacc gggtccacaa acgggagagc cggttattgc tcctgcaccg
    30241 gaaggttacc cacagcagtc acaatatgtg cagcctgcag cgcaatataa tgagccgctg
    30301 caacaaccag tacagccgca gcagccgtat tatgcacctg cagctgaaca accttactac
    30361 gctcctgcgg cggaacaacc tgttcagcaa ccgtattacg caactgcgcc ggaacagcct
    30421 gcgcaacagc cgtattatgc ccctgcgcca gaacaaccgg tggcaggtaa cgcctggcaa
    30481 gccgaagagc agcaatccac ttttgctcca cagtctacat accagactga gcaaacttat
    30541 cagcagccag ccgctcagga gccgttgtac caacagccgc aacctgttga acagcagcct
    30601 gttgtggagc ctgaacccgt tgtagaagag acaaaacccg cgcgtccgcc gctttactac
    30661 tttgaagaag tggaagagaa gcgagcccgt gaacgtgaac aacttgcggc ctggtatcaa
    30721 ccgattccag agccggttaa agaaccagaa ccgatcaaat cttcgctgaa agcaccttct
    30781 gttgcagcag tacctccagt agaagctgct gccgctgttt ctccgctggc atctggcgtg
    30841 aaaaaagcga cactggcgac gggggctgcc gcaaccgttg ccgcgccagt cttcagtctg
    30901 gcaaatagcg gtggaccgcg tccgcaggtc aaagagggga ttggcccgca gttgccacga
    30961 ccgaaacgta tccgcgtgcc aactcgtcgt gaattggcgt cttacggtat taagctgccc
    31021 tcacagcgtg cggcggaaga aaaagcccgt gaagcccagc gcaatcagta tgattctggc
    31081 gatcagtata atgatgatga aatcgatgcg atgcagcagg atgaactggc acgtcagttc
    31141 gcccagacac agcagcaacg ctatggcgaa cagtatcaac atgatgtgcc cgtaaacgca
    31201 gaagatgcag atgctgcggc agaggctgaa ctggctcgtc agtttgcgca aactcaacaa
    31261 caacgttatt ccggcgaaca accggctggg gcgaatccgt tctcgctgga tgattttgaa
    31321 ttttcgccaa tgaaagcgtt gctggatgat ggtccacacg aaccgttgtt tacgccaatt
    31381 gttgaacctg tacagcagcc gcaacaaccg gttgcaccgc agcagcagta tcagcagcca
    31441 caacagccgg ttgcgccaca gcagcaatat cagcagccgc aacaaccggt tgcaccgcag
    31501 cagcaatatc agcagccgca acaaccggtt gcaccgcagc cgcaatatca gcagccgcaa
    31561 cagccagttg cgccgcagcc gcagtatcag cagccacaac aatcggctgc accacagccg
    31621 caatatcagc agccgcaaca gccagttgcg ccacagccgc aggatactct gcttcatccg
    31681 ctgttgatgc gtaatggcga cagccgtccg ttgcataaac cgacgacgcc gctgccttct
    31741 ctggatttgc tgacaccgcc gccgagcgaa gtggagccgg tagatacctt tgcgcttgaa
    31801 caaatggcgc gtctggtgga agcacgtctg gctgatttcc gtattaaagc cgatgtcgtc
    31861 aattactctc cggggccggt tatcactcgc tttgaactta acctggcacc gggcgtaaaa
    31921 gcggcgcgca tttctaactt gtcacgggac cttgcccgtt cactttcgac ggtggcggtg
    31981 cgtgtcgttg aagttattcc tggcaaaccc tatgtaggtc tggagttacc gaataaaaaa
    32041 cgacaaaccg tttatctgcg cgaagtattg gataacgcca aattccgcga taatccatcg
    32101 ccattaaccg tggtgctggg taaagatatc gccggtgagc cggtggttgc cgatctggcg
    32161 aaaatgccgc acttgttagt tgcggggact accggttccg gtaaatctgt cggtgtgaac
    32221 gcgatgatcc tgagcatgct ttataaagca cagccagaag atgtgcgttt catcatgatc
    32281 gacccgaaaa tgctggagct ttcggtgtat gaaggcattc cgcacctgtt aacggaagtc
    32341 gttactgata tgaaagatgc cgccaacgcg ctgcgctggt gtgttaacga gatggagcgt
    32401 cgttataaac tgatgtctgc gctgggtgtg cgtaatctgg cgggttataa cgaaaaaatt
    32461 gctgaagccg atcgcatgat gcgtccgatt ccagacccgt actggaagcc gggtgacagt
    32521 atggatgccc agcatccggt gctgaaaaaa gaaccataca ttgtggtgtt ggtggacgaa
    32581 tttgccgacc tgatgatgac agtaggtaaa aaagtggaag agctgatagc acgtctggcg
    32641 caaaaagccc gtgccgcggg tatccacctc gtactggcaa ctcagcgtcc atcggttgat
    32701 gttattactg gtctgattaa agcgaatatt ccgacccgta tcgcctttac cgtatccagt
    32761 aagattgact cacgtaccat tcttgatcag gctggcgcgg aatcactgct gggtatgggg
    32821 gatatgctct actccgggcc gaactccacg ttgccggtac gtgtccatgg tgcttttgtt
    32881 cgcgatcagg aagttcatgc cgtggtgcag gactggaaag cgcgtggtcg cccacagtat
    32941 gttgatggca tcacctccga cagcgaaagc gaaggtggtg cgggtggttt cgatggcgct
    33001 gaagaactgg atccgttgtt cgatcaggcg gtgcagtttg tcactgaaaa acgcaaagcg
    33061 tcaatttctg gcgtacagcg tcagttccgc attggttata accgtgcagc gcgtattatc
    33121 gaacagatgg aagcgcaggg aattgtcagc gaacaggggc acaacggtaa tcgtgaagtg
    33181 ctggccccac cgccgtttga ctaattaatg cattgccgga taaggcgcgg tagcgtcgca
    33241 tccggcactc tatcaactga aaattcagta ttttcttctt tcctcaagct gattattagc
    33301 ctggaataga gagtagaggg aactcccgat cgggagtgac gtaatttgag gaataatgat
    33361 gaaaaaaatt gccatcacct gtgcattact ctcaagctta gtagcaagca gcgtttgggc
    33421 tgatgccgca agcgatctga aaagccgcct ggataaagtc agcagcttcc acgccagctt
    33481 cacacaaaaa gtgactgacg gtagcggcgc ggcggtgcag gaaggtcagg gcgatctgtg
    33541 ggtgaaacgt ccaaacttat tcaactggca tatgacacaa cctgatgaaa gcattctggt
    33601 ttctgacggt aaaacactgt ggttctataa cccgttcgtt gagcaagcta cggcaacctg
    33661 gctgaaagat gccaccggta atacgccgtt tatgctgatt gcccgcaacc agtccagcga
    33721 ctggcagcag tacaatatca aacagaatgg cgatgacttt gtcctgacgc cgaaagccag
    33781 caatggcaat ctgaagcagt tcaccattaa cgtgggacgt gatggcacaa tccatcagtt
    33841 tagcgcggtg gagcaggacg atcagcgcag cagttatcaa ctgaaatccc agcaaaatgg
    33901 ggctgtggat gcagcgaaat ttaccttcac cccgccgcaa ggcgtcacgg tagatgatca
    33961 acgtaagtag aggcacctga gtgagcaatc tgtcgctcga tttttcggat aatacttttc
    34021 aacctctggc cgcgcgtatg cggccagaaa atttagcaca gtatatcggt cagcaacatt
    34081 tgctggctgc ggggaagccg ttgccgcgcg ctatcgaagc cgggcattta cattctatga
    34141 tcctctgggg gccaccgggt accggcaaaa caaccctcgc tgaagtgatt gcccgctatg
    34201 cgaacgctga tgtggaacgt atttctgccg tcacctctgg cgtgaaagag attcgcgagg
    34261 cgatcgagcg cgcccggcaa aaccgcaatg caggtcgccg cactattctt tttgttgacg
    34321 aagttcaccg tttcaacaaa agccagcagg atgcattcct gccacatatt gaagacggca
    34381 ccatcacttt tattggcgca accactgaaa acccgtcgtt tgagcttaat tcggcactgc
    34441 tttcccgtgc ccgtgtctat ctgttgaaat ccctgagtac agaggatatt gagcaagtac
    34501 taactcaggc gatggaagac aagacccgtg gttatggtgg tcaggatatt gttctgccag
    34561 atgaaacacg acgcgccatt gctgaactgg tgaatggcga cgcgcgccgg gcgttaaata
    34621 cgctggaaat gatggcggat atggccgaag tcaatgatag cggtaagcgg gtcctgaagc
    34681 ctgaattatt gaccgaaatc gccggtgagc gaagcgcccg ctttgataac aaaggcgatc
    34741 gcttttacga tctcatttcc gcactgcata agtcggtacg tggtagcgca cccgatgcgg
    34801 cgctctactg gtatgcgcga attattaccg ctggtggcga tccgttatat gtcgcgcgtc
    34861 gctgtctggc gattgcgtct gaagacgtcg gtaatgccga tccacgggcg atgcaggtgg
    34921 caattgcggc ctgggattgc tttactcgcg ttggcccggc ggaaggtgaa cgcgccattg
    34981 ctcaggcgat tgtttacctg gcctgcgcgc caaaaagcaa cgctgtctac actgcgttta
    35041 aagccgcgct ggccgatgct cgcgaacgcc cggattatga cgtgccggtt catttgcgta
    35101 atgcgccgac gaaattaatg aaggaaatgg gctacgggca ggaatatcgt tacgctcatg
    35161 atgaagcaaa cgcttatgct gccggtgagg tttacttccc gccggaaata gcacaaacac
    35221 gctattattt cccgacaaac aggggccttg aaggcaagat tggcgaaaag ctcgcctggc
    35281 tggctgaaca ggatcaaaat agccccataa aacgctaccg ttaatgttat cgttgcggta
    35341 atgttgttac tgtatccctg tggtcgcagg ctgtggccac atctcccatt taattagata
    35401 agcacaggat aagcatgctc gatcccaatc tgctgcgtaa tgagccagac gcagtcgctg
    35461 aaaaactggc acgccggggc tttaagctgg atgtagataa gctgggcgct cttgaagagc
    35521 gtcgtaaagt attgcaggtc aaaacggaaa acctgcaagc ggagcgtaac tcccgatcga
    35581 aatccattgg ccaggcgaaa gcgcgcgggg aagatatcga gcctttacgt ctggaagtga
    35641 acaaactggg cgaagagctg gatgcagcaa aagccgagct ggatgcttta caggctgaaa
    35701 ttcgcgatat cgcgctgacc atccctaacc tgcctgcaga tgaagtgccg gtaggtaaag
    35761 acgaaaatga caacgttgaa gtcagccgct ggggtacccc gcgtgagttt gactttgaag
    35821 ttcgtgacca cgtgacgctg ggtgaaatgc actctggcct cgactttgca gctgcagtta
    35881 agctgactgg ttcccgcttt gtggtaatga aagggcagat tgctcgcatg caccgcgcac
    35941 tgtcgcagtt tatgctggat ctgcataccg aacagcatgg ctacagtgag aactatgttc
    36001 cgtacctggt taaccaggac acgctgtacg gtacgggtca gttgccgaaa tttgctggcg
    36061 atctgttcca tactcgtccg ctggaagaag aagcagacac cagtaactat gcgctgatcc
    36121 caacggcaga agttccgctg accaacctgg tgcgcggtga aatcatcgat gaagatgatc
    36181 tgccaattaa gatgaccgcc cacaccccat gcttccgttc tgaagccggt tcatatggtc
    36241 gtgacacccg tggtctgatc cgtatgcacc agttcgataa agttgaaatg gtgcagatcg
    36301 tgcgcccgga agactcaatg gcggcgctgg aagagatgac tggtcatgca gaaaaagtcc
    36361 tgcagttgct gggcctgccg taccgtaaaa tcatcctttg cactggcgac atgggctttg
    36421 gcgcttgcaa aacttacgac ctggaagtat ggatcccggc acagaacacc taccgcgaga
    36481 tctcttcctg ctccaacgtt tgggatttcc aggcacgtcg tatgcaggca cgttgtcgca
    36541 gcaagtcgga caagaaaacc cgtctggttc ataccctgaa cggttctggt ctggctgttg
    36601 gtcgtacgct ggttgcggtt atggaaaact atcagcaggc tgatggtcgt attgaagtac
    36661 cagaagttct gcgtccgtat atgaacggac tggaatatat tggctaatac ccaatttttc
    36721 tgaatctaaa aagcgcctac gggcgctttt tttgtctccc tttgataccg aacaataatt
    36781 actcctcact tacacgtaat actactttcg agtgaaaatc tacctatctc tttgattttc
    36841 aaattattcg atgtatacaa gcctatatag cgatctgcta tagaaataat tacacaatac
    36901 ggtttgttac tggaatcaat cgtgagcaag cttgagtgag ccattatgaa aacgaaaatc
    36961 cctgatgcgg tattggctgc tgaggtgagt cgccgtggtt tggtaaaaac gacagcgatc
    37021 ggcggcctgg caatggccag cagcgcatta acattacctt ttagtcggat tgcgcacgct
    37081 gtcgatagcg ccattccaac aaaatcagac gaaaaggtta tctggagcgc ctgtacagtt
    37141 aactgtggta gtcgctgccc gctacgtatg cacgtcgtgg acggtgaaat caaatatgtc
    37201 gaaacggaca ataccggcga tgacaattac gacggcctgc accaggttcg cgcctgtttg
    37261 cgtgggcgtt ccatgcgtcg ccgtgtctat aatccggacc gcctgaaata tccgatgaaa
    37321 cgagtcgggg cgcgcggtga aggcaaattt gagcgcatta gctgggaaga agcctacgac
    37381 atcatcgcga ccaatatgca gcgcctgatc aaagagtacg gcaacgaatc catctatctg
    37441 aactatggca ccggtacgct gggcggcacc atgacccgct cctggccccc gggaaatacc
    37501 ctggtcgcgc gcctgatgaa ctgctgcggc ggttatctga accattacgg tgactactct
    37561 tccgcgcaaa ttgcggaagg tttgaactat acctacggcg gctgggcaga tggtaacagc
    37621 ccgtcggata tcgaaaacag taaactggta gtgctgtttg gtaataaccc tggcgaaacg
    37681 cgaatgagtg gcggtggggt gacttactat cttgaacagg cacgccagaa atctaatgcc
    37741 cgcatgatca tcatcgatcc gcgctatacc gacaccggtg ccgggcgcga agatgagtgg
    37801 atccctattc gtccaggaac agatgccgca ctggttaacg gtctggcgta tgtcatgatc
    37861 accgaaaacc tggtggatca ggcattcctc gataaatatt gcgttggcta cgatgagaaa
    37921 accctgccag ccagtgcgcc gaaaaatggc cactataaag cttatattct gggtgaaggg
    37981 ccagatggcg tggctaaaac accggaatgg gcctcgcaaa tcaccggtgt tccggcagac
    38041 aaaatcatca aactggctcg tgaaatcggc agtaccaaac cggcgtttat cagccaggga
    38101 tggggcccgc agcgtcacgc taacggtgaa atcgcaaccc gtgctatctc gatgctggcg
    38161 attctgaccg gtaacgttgg tattaatgga gggaacagcg gcgcgcgtga aggttcatac
    38221 agcttaccgt ttgtccgtat gccgactctg gaaaacccga tccagaccag catttctatg
    38281 tttatgtgga ccgatgccat tgaacgtggc ccggaaatga cggcgctgcg tgatggtgtg
    38341 cgcgggaaag ataagctgga tgtgccgatc aaaatgatct ggaactatgc cggtaactgc
    38401 ctgattaacc agcattctga gatcaaccgt acccatgaaa tccttcagga tgataagaag
    38461 tgcgagctga ttgtggttat cgactgccac atgacttcat cggcgaaata tgctgacatc
    38521 ctgctgcctg actgtaccgc ttccgaacag atggactttg cactggatgc atcctgcggg
    38581 aatatgtctt acgtgatttt caacgatcag gtgattaaac cgcgctttga atgtaagacc
    38641 atctatgaaa tgaccagcga actggcaaaa cgtcttggcg ttgagcaaca gtttactgaa
    38701 ggccgtaccc aggaagagtg gatgcggcat ctgtatgccc agtcgcggga agcgattcct
    38761 gaactgccaa cgtttgaaga gttccgcaag caggggatct ttaaaaagcg cgacccacaa
    38821 gggcatcacg ttgcttataa agccttccgt gaagatccgc aggcaaaccc actgactacg
    38881 ccatcgggca aaattgagat ttattcgcag gcgctggctg acattgccgc tacctgggaa
    38941 ttgccagacg gcgatgtgat cgatccactg ccgatctaca cgccgggctt tgaaagttat
    39001 caggatccgc tgaacaaaca gtatccgctg cagcttacgg gtttccacta taaatctcgc
    39061 gttcactcaa cttacggcaa cgttgatgtg ctgaaagcgg cttgccgtca ggaaatgtgg
    39121 atcaacccgc ttgatgccca aaaacgtggt atccacaacg gcgataaagt caggatcttt
    39181 aacgatcgtg gtgaggttca tattgaggcg aaagtgacgc cacgaatgat gccgggtgtg
    39241 gtcgcactgg gtgaaggtgc ctggtatgac ccggatgcaa aacgtgtcga taagggtggt
    39301 tgtattaacg tactgaccac tcaacgtccg tctcctctcg ctaaggggaa tccgtcacat
    39361 acaaaccttg ttcaggttga aaaggtgtaa ggagtaaccg atgacaaccc agtatggatt
    39421 ttttattgat tccagccgtt gcaccggttg caaaacctgc gagctggctt gtaaagacta
    39481 caaagatttg acgccagaag tcagcttccg ccgcatttat gaatatgctg gcggcgactg
    39541 gcaggaagat aacggtgtct ggcaccagaa cgtgtttgcc tactatctgt cgatttcatg
    39601 taaccactgc gaagatccgg cttgtactaa agtctgcccg agcggtgcga tgcataaacg
    39661 tgaagatggt tttgttgtgg tcgatgaaga tgtatgcatt ggctgccgtt actgccatat
    39721 ggcttgcccg tacggcgcac cgcaatataa cgaaacgaaa ggccatatga ccaaatgcga
    39781 tggttgttat gaccgtgttg cggagggtaa aaagccgatc tgtgttgaat cttgcccgct
    39841 gcgggcgctg gatttcggcc ctatcgacga gctgcgtaaa aaacatggcg atctggcggc
    39901 cgttgcgccg ttgccgcgag ctcactttac caaaccgaat attgtgatca aacccaatgc
    39961 caacagccgc ccgaccgggg ataccactgg ctacctggca aacccgaagg aggtgtgaga
    40021 tgggaagtgg atggcatgaa tggccgctga tgatcttcac ggtcttcggg caatgtgtag
    40081 cgggtggttt tatcgttctg gctttggcgc tgctcaaagg cgacctgcga gcagaagccc
    40141 agcagcgtgt tatcgcctgc atgtttggtt tatgggtgct gatgggcatt ggctttatcg
    40201 cctctatgct ccatcttggt tcaccaatgc gcgcttttaa ctcgctcaac cgggtagggg
    40261 cttcagcact cagtaacgaa atcgccagcg gttcgatctt ttttgccgta ggcggcatcg
    40321 gctggttgct ggcaatgctg aaaaagctgt caccggcatt gcgtacgctg tggctggtgg
    40381 tgacgatggt tcttggcgtc gtctttgtct ggatgatggt gcgtgtgtat aacagcattg
    40441 ataccgttcc gacctggtac agcatctgga cgccgatggg cttcttcctg acgatgttta
    40501 tgggcggccc gttactgggt tacctgttgc tgagtctggc tggcgtcgat ggctgggcga
    40561 tgcgtctgct gccagccatt tctgtactgg cactggtggt gagtggcgtg atgtcagtga
    40621 tgcagggcgc agagctggcg actattcata gttctgtgca gcaggccgca gcgctggtgc
    40681 cagactatgg tgcgctgatg tcctggcgga tcgtgctttt ggccgttgcc ctgtgcttgt
    40741 ggattgcacc acagctaaaa ggttatcagc ctgcggttcc gctactatcc gtctcattca
    40801 ttctgcttct ggcaggggaa ttaatcggtc gcggcgtatt ctatggtttg catatgaccg
    40861 tggggatggc cgtcgcaagc taatcagtaa caaccggggt ttcggccccg gttctctttt
    40921 atttctgctt cgttaacgtg tcataactgg tcatcaaatt acgataatca ggaatatggt
    40981 tagagaacaa cgtcgccagt ccttcaatat cattacgcca gtcacggtgc agctcgcaag
    41041 ccacgccgaa ccatgtcatc aattgcgcac ctgcttgcga catgcgatcc catgccgaat
    41101 gccgggtaat ttcattaaag gtccctgacg cgtcagtcac aacaaagaca tcaaaccctt
    41161 cttcaatggc tgaaagcgcc gggaatgcca cgcaaacttc ggttaccaca ccggcaataa
    41221 ttaactgttt tttacctgtc gctttgactg cttttacaaa atcttcgtta tcccaggcgt
    41281 taatatttcc cgggcgagca atgtaaggtg catcggggaa ttgtgctttc agttctggaa
    41341 ctaatggacc gttaggaccg gtttcaaaac tggtggtaag aatagtaggt aagttgaaat
    41401 acttagccag gtcacccagc gccagcacgt tatttttaaa cttatcgggt tcgatatccc
    41461 gtacaaggga aagtaaacca gcctgatgat caacaagcaa aacggcagca tcatttttat
    41521 caagacgaac atacggtttg gtcatcctct tctcctttcg agagtcagtg gtacgtctga
    41581 aatcggtcag acgtgatgca ttgctctgaa agcatagacg ggaaatatga gtttgctgtg
    41641 accatgaaat ttttcgactg aacgaagcgt atgaaatttg tgttagatca ataaaaacaa
    41701 tcagatgaga ttaatcatct cttcagcatt agcgttatga atgagtaata taaatcgata
    41761 ataccgccgc tgcgatatgc gcgtattttc acccattgac aatgtttttg gcggtggcat
    41821 gatgcgcatg aaatttgaac ttcctcacgg ttttaattca tgtccacgta tacccggcct
    41881 gtcatgcttt tgctgtctgg cctgcttttg ttgactctgg cgatcgcggt gttaaataca
    41941 ctcgtgccgc tttggctcgc ccaggaacac atgtccacat ggcaggtagg cgttgtcagc
    42001 tcatcctatt ttaccggcaa ccttgtcggt acattgctga cagggtatgt cattaaacgc
    42061 attggcttta accgcagcta ttatctggcc tccttcattt ttgccgctgg ctgtgccggc
    42121 cttggcctga tgattggatt ctggagttgg ttggcgtggc gttttgtcgc gggcgtcggc
    42181 tgtgccatga tttgggtagt cgttgagagc gcgctgatgt gcagtgggac gtcacgtaac
    42241 cgtgggcgtt tgcttgctgc atacatgatg gtttattacg tgggaacgtt tttaggccag
    42301 ttactggtca gcaaggtctc aaccgagctg atgagcgtat tgccgtgggt tacaggtttg
    42361 acattggcag ggatcttacc gctgttgttt acgcgtgtgc tgaatcagca ggctgaaaac
    42421 catgattcga cgtcaattac ggcaatgcta aaactccgtc aggcgcggct tggcgtgaat
    42481 ggctgcatta tctcaggaat cgttctggga tctctatatg gcctgatgcc gttgtacctc
    42541 aatcacaaag gggtgagcaa tgccagcatt ggtttctgga tggcggtact ggtcagtgcg
    42601 ggtatcctcg gacaatggcc gatcggacgt ctggcggata agtttggtcg actgctggtg
    42661 ttgcgtgttc aggtttttgt cgtcattctc ggcagtatcg cgatgcttag ccaggcggcg
    42721 atggccccag cgttattcat cctcggtgcc gctggcttta cgctatatcc ggtggcgatg
    42781 gcctgggctt gtgagaaagt tgaacatcat caactggtgg cgatgaacca ggccttactg
    42841 ttgagctata ccgtgggaag tctgcttggc ccgtcattta ccgctatgct aatgcagaat
    42901 ttctccgata atttattgtt tatcatgatc gccagcgtat cgtttatcta tttgctgatg
    42961 ctgcttcgca acgccggtca tacgccgaaa cccgttgctc acgtgtaaat gaattcaagc
    43021 agagtgtgaa cttactgttt cacactctgc ttttttgttt cttctatctg acttgctttt
    43081 ttccaaattt tattcgttta aaaataaaat gtgcagcagg ttataatttt gcatttcgct
    43141 atttccgcac ttcttatttg ccgcgcataa tccctcgttt tacagatgcc cctttaattt
    43201 tggcgaagga tttgtctatg gctgggaatg ttcaggaaaa acagctgcga tggtacaaca
    43261 ttgcgctgat gtcttttatc actgtctggg gttttggcaa cgttgttaat aactatgcca
    43321 accaggggct ggtggttgtt ttttcatggg tgtttatctt tgcgctctat ttcacacctt
    43381 atgcgctaat tgttggtcag ttaggctcga ccttcaaaga tgggaaaggc ggggtcagta
    43441 cctggattaa acacacgatg ggacccggac tggcttacct cgccgcgtgg acctactggg
    43501 tggtgcatat tccctatctg gcacaaaaac cccaggcaat actgattgcg ctcggttggg
    43561 cgatgaaagg cgacggttcg ctaatcaaag aatattcagt cgtagcgtta caggggttaa
    43621 cgctggtgct gtttatcttc tttatgtggg ttgcttcacg cggtatgaaa tcgctgaaaa
    43681 tcgtcggttc tgtggcaggg attgcgatgt ttgttatgtc gctcctgtat gtggcgatgg
    43741 cggtaaccgc gcctgcgatt actgaagtgc atattgcgac cacaaacatt acctgggaaa
    43801 cgttcattcc tcatatcgac tttacctaca ttaccactat ttcaatgctg gttttcgcgg
    43861 ttggcggagc agagaagatt tctccttacg ttaatcaaac gcgcaaccca ggaaaagaat
    43921 ttccaaaagg gatgttatgc ctggcggtga tggttgcggt ttgtgccatt ctgggctcac
    43981 tggcgatggg gatgatgttt gactcgcgta atatcccgga tgacttaatg actaacggtc
    44041 aatattacgc ctttcagaag ctgggcgagt attacaacat gggtaatact ttaatggtga
    44101 tttacgccat tgcgaatacc ctgggacaag tagctgcgct ggtattctcg attgatgccc
    44161 cgcttaaagt gctattaggt gatgctgaca gcaaatatat tccagccagt ttatgtcgta
    44221 ccaacgcttc tggtacgccc gttaatggct attttctgac cctggtgctg gtggcaattc
    44281 tgattatgct gccgactctc ggcatcggtg atatgaacaa tctctataaa tggctgttga
    44341 atcttaactc ggtagtgatg ccgctgcgtt atttatgggt atttgttgca tttattgcag
    44401 tcgttcgcct ggcgcagaaa tataaaccag aatatgtctt tattcgtaat aagcctctgg
    44461 caatgaccgt cgggatttgg tgttttgcct ttaccgcctt tgcctgtttg acggggatct
    44521 tcccgaaaat ggaagccttc actgcagagt ggaccttcca gttggcgctg aatgttgcaa
    44581 cgccgtttgt gctggtagga ttaggactga tattcccgct gctggcgcgt aaagcgaata
    44641 gtaaataatt attgtggtcg gtgacgctga ctctggcaga tctgacccgt ttttacataa
    44701 tcgagaaatg cgcgcaagcc gcaggacata tactggcggt ttgggtaata gacctggaag
    44761 cctggacgct ctgtgatcca gtcttccagt acacattcta aacgaccagt atcaaggtac
    44821 tccttgatct gttcatagag cagatacccg ataccagcac ccatcaaaac ggcctccagt
    44881 tcagcatcaa catcatccag aatgatattt cctgcgacag caatttccag ctcctttgca
    44941 aattgccagt ggaaaggttt gccgctggga taacgaaaaa caacacattg atgattaagc
    45001 agatcatgag gatggcgggg tttgccatat cttgcaaagt actctggcgt tgctgctaca
    45061 cataatttca ctggtgggcc gatcgccacg ctgatcatat ctttttcgac gatgccacta
    45121 agtcgaacgc cagcgtcaaa gccctgttgg acgatatcga caagagagtc gtcagttgtg
    45181 agctcgactt taatatcagg atattcacgt gtgaaaccaa ccagtaaagg cattaaaaat
    45241 atacgtgcag ctacccgggc ggcattgatt ttgagtgtgc ctgtcggtgt cagacgaaaa
    45301 tcattcatct catctaacat gatctgaatt tcgtcgaaag ctgggcgcaa tcgttcatag
    45361 agattagaac ccgcttccgt taacgatacg cttcgggttg tccgattgaa taagcgaatt
    45421 ttaagacgtt gttccagtgt tttaatacta tggctaatgg cggacgagga taagcctaac
    45481 tcatcgcccg ctgcacgaaa gctttgatta cgggccacgg caaagaaagt ggcaaagtca
    45541 gacatattca tccgcattag tgaaaatcct tcattaactc atcctgaatc atcacgttta
    45601 caggattatg ccgcctgcgt aaagtgcgct ccagaactta acgtggaggt aaaattatgc
    45661 agtctgaacg tatttatttg gtatgggccc atcctcgtca tgattcattg accgcaaata
    45721 ttgctgatgc gatccatcag cgggcaatgg agcggaaaat acaggtgacg gaactcgatt
    45781 tatatcggcg taatttcaac ccagtgatga cgccggaaga tgaaccagac tggaagaata
    45841 tggataaacg ttattctcca gaggttcatc agctttattc agagctgctt gaacatgaca
    45901 cgttagtggt ggtttttcct ctctggtggt acagcttccc ggcaatgctg aaaggatata
    45961 ttgacagagt atggaataat gggctggctt atggagatgg gcacaaatta ccattcaata
    46021 aagttcgttg ggtggcgctg gttggaggag acaaagaatc atttgtccag atgggctggg
    46081 aaaaaaatat aagcgattat ttgataaata tgtgcagtta tcttggtatt gaagatgccg
    46141 atgtcacttt cttgtgtaat acagtggtat tcgatgggca agaacttcat gcgagctatt
    46201 atcagtcgtt attatctcag gtacgggata tggtagatgc actataaaat gtgttaaaaa
    46261 cgctgtagca gaatgaagcg cggaataaaa aagcggcaac tcaataaagt tgccgcttta
    46321 tggggaaatt agaacattac cttatgaccg tactgctcaa gaatgccttt cacgcgttcc
    46381 atggtttctt tcttcggtgg tttaacacca tcgagtttgt attcttcacc catcgccacc
    46441 catttgtgtt tgcccagttc gtggtagggg agaagttcga ttttctcaac gttacccata
    46501 tcacgggtaa attcaccgag gcgatgcgct gaatcgtcat cgtcagacca gcctgggaca
    46561 acaacgtagc ggatccacac cttcacattt ttattcgcca gatatttggc gaactccagc
    46621 gtgcggtggt tggaaactcc aaccagattt tggtggatct cgtcgttcat ctgtttgaga
    46681 tcgagcatta ccaggtcggt tacttccagc agttcatcaa tcaccggatc gtaacgacga
    46741 acaaaaccgt tggtgtccag acaggtatga atgccttctt ttttgcaggc gcggaaccag
    46801 tcacgaacaa actcagcttg caggattgcc tcaccgccgg atgcggtaac gccgccgccg
    46861 gaagcgttca taaagtggcg ataggtcacc acttccttca tcaaatcttc aacggtaact
    46921 tctttaccgc catgcgtgtc ccaggtgtcg cggttatgac aatacaggca gcgcatcagg
    46981 cagccctgga aaaaggtgat aaagcgaata cctgggccgt ctacggttcc acaggattca
    47041 aaggagtgaa tgcgaccaat aactgacatt gcggtgtttc tccagatgtg gcccatctga
    47101 ggccgtgttg gtgcgcagct cgaaggctac gtcgagtctg ttttggcagt caccttaaag
    47161 tatagatagc tgacaaaaaa ggctctcgtg ctaaaaaagg ccccactttc gtggagcctt
    47221 tattgtacgc tttttactgt acgatttcag tcaaatctaa ttacatagat tgagtgaagg
    47281 tacgagtaat aacgtcctgc tgctgttctt tagtcagcga gttgaaacgt actgcgtagc
    47341 cagatacacg gatggtcagc tgcggatatt tttccgggtt ttccatcgcg tcgagcagca
    47401 tttcacggtt catcacgtta acgttcaggt gctgaccacc ttcgatggat gcttcgtggt
    47461 ggaagtaacc atccatcaga ccagccaggt tggtcttacg aacttcgtcg tctttaccca
    47521 gtgcgttcgg aacgatagag aaggtgtagg agataccatc tttagcgtaa gcaaacggca
    47581 gtttagcaac ggaagtcaga gacgctacag cacctttctg gtcacgaccg tgcatcgggt
    47641 tagcacccgg tccgaacggc gcgccagcac gacgaccgtc tggggtgtta ccagttttct
    47701 taccatacac aacgttagac gtgatggtca gaacagactg agtcgggata gcgtcacggt
    47761 aggtgtgcag tttctgaatt ttcttcatga aacgttctac caggtcaaca gccaggtcat
    47821 ctacacgcgg atcgttgtta ccaaactgcg ggtattcgcc ttcgatttcg aagtcgatag
    47881 ccagaccgtc ttcgtcacga atcggtttaa ctttcgcata tttgattgca gacagggagt
    47941 cagcagcaac ggacagacca gcgataccac acgccatggt gcggataacg tcacggtcgt
    48001 gcagcgccat cagagaggct tcgtagctgt acttgtcgtg catgtagtgg atgatgttca
    48061 gtgcagtgat gtactgttta gccagccagt ccatgaagtg atccatgcgc tccatcactt
    48121 catcgtagtt caggagatcg cctttgatcg gctcagactt cggaccaacc tgcattttca
    48181 gtttttcgtc aacgccgccg ttgattgcgt acagcatggt tttcgccagg tttgcacgcg
    48241 caccgaagaa ctgcatttgt ttaccaacga tcatcgggct tacgcagcaa gcgatagcgt
    48301 agtcatcgtt gttgaagtcc ggacgcatca ggtcatcgtt ctcatactgc agagaagagg
    48361 tgtcgatgga cactttagcg gcgaatttct tgaagttcag cggcagtttt tcagaccaca
    48421 gaatggtcat gttcggttcc ggagacggcc ccatggtgta cagggtgttc aggaaacgga
    48481 agctgttttt ggtaaccagg gtacgaccgt cgaggcccat accaccgata gattcggttg
    48541 cccagatcgg gtcgccagag aacagttcat cgtactccgg agtacgcagg aagcgaacca
    48601 tacgcagttt catgaccagg tggtcaacca tttcctgcgc ttcttgttcg gtgatcttgc
    48661 cagctttcag gtcacgttcg atgtacacat ccaggaaggt ggaggtacga ccgaaggaca
    48721 ttgcagcacc gttctgagac ttaacagcag ccaggtagcc gaagtaagtc cactggatag
    48781 cttcctgagc gttggtagcc ggaccagaga tgtcgtagcc gtatttcgca gccatttctt
    48841 tcatctgacc cagagcgcgg tgctgttcag cgatttcttc gcgcagacgg atagtctgtt
    48901 ccaggtttac gccgttttcc agatcagcct gcagagaggt gaactgagcg tatttgtctt
    48961 tcatcaggta gtcgataccg tacagcgcaa cgcgacggta gtcaccgatg atacggccac
    49021 ggccataagc atctggcaga ccggtcagaa caccggattt acggcaacgc aggatgtccg
    49081 gagtgtaaac gtcgaacacg ccctggttgt gagttttacg gtattcagtg aagatttttt
    49141 tgatcatcgg gtccagttcg cggttgtacg ctttgcagga accttcgatc attttgatac
    49201 caccgaacgg gataagagca cgtttcagcg gagcttcagt ctgcagacca acaacttttt
    49261 ccaacgcttt gttgatgtag ccagcgtcgt gagaggtgat ggtggaagca acagcggtgt
    49321 caaagtcaac tggcgcgtga gtgcggtttt ccagtttaac gccttccatt actttgtccc
    49381 acagggtggt ggtcgcttca gtagcgccag ccaggaagga ctcgtcaccc tcgtacggag
    49441 tgtagttttt ctgaatgaag tcacggacgt ttacttcatt ctgccagtca cctttggtaa
    49501 aaccttccca ggctgtggct aacttttcat taagctcgga catgtaacac ctaccttctt
    49561 aagtggattt tttatttact gcgtacttcg acaaccatta atggtgatcg ttttcacgca
    49621 ggtaaatgac ccagtatgtc aacccaacca acaaaccacc gccgataata ttaccgatcg
    49681 taaccggaat caggttatca gtgatgaagt tcatcacggt caggtgagaa aaattttccg
    49741 gtgcagaacc gacagcggtc cagaattccg gagatgcgaa gtcgcggatt acaataccca
    49801 tcgggatcat aaacatgttt gcgatactgt gctcaaaacc gctggcaaca aacatcgcga
    49861 ccggcagcac catgataaac gctttgtcca tcaggctgcg gccagaataa ctcatccata
    49921 ctgccagaca taccatcagg tttgccagga taccaaggca gacggcctca ataaaagtat
    49981 ggtgcacttt gtggtcggcg gtttgtagga cgtttagtcc ccattgacca tttgcggtca
    50041 tatactcgcc ggaaagccac attaaaagta caaacagcag tgcgccgacc aggttgccaa
    50101 aatagacatt tagccagttt ttagccaact gaccccaggt gatgcgccca ctcgccttag
    50161 caacaacaat caacactgtg gaagtaaaga gatcggctcc gcagacaaca caaagaatca
    50221 gccccagaga gaagcaaatg ccgccaacca gttttgccat gccgaagggc attgtgcctg
    50281 tgccagtggt tgctgtgata tagaagacga atgcgattga gatgaaaaca ccggcggtaa
    50341 tcgccagata gaaagtctta agcggatgtt tcgttgcttt atagacaccc gcctcttcgg
    50401 ccactttggc cattgcagca ggaagtaaaa gatcaaaagg gttgtcagct ttcacactaa
    50461 ctctctcttt attaagtcgg cgacgagata ctaacaaagc attatagatg agaaattgat
    50521 atagatcata tctcgcctgg cttataggcc cgtaactcgc atggtttt
//
LOCUS       DAPGOF010000010        48488 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.010, whole genome shotgun sequence.
ACCESSION   DAPGOF010000010 DAPGOF010000000
VERSION     DAPGOF010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.010"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            235..1452
                     /gene="alaA"
                     /locus_tag="RHV13_000495"
     CDS             235..1452
                     /gene="alaA"
                     /locus_tag="RHV13_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HDV1240055.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1536..2135
                     /gene="yfbR"
                     /locus_tag="RHV13_000496"
     CDS             1536..2135
                     /gene="yfbR"
                     /locus_tag="RHV13_000496"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HDV1240056.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELQDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2194..4026)
                     /locus_tag="RHV13_000497"
     CDS             complement(2194..4026)
                     /locus_tag="RHV13_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HDV1240057.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LACLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4113..4763)
                     /gene="hxpA"
                     /locus_tag="RHV13_000498"
     CDS             complement(4113..4763)
                     /gene="hxpA"
                     /locus_tag="RHV13_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HDV1240058.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVNLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4774..5268)
                     /locus_tag="RHV13_000499"
     CDS             complement(4774..5268)
                     /locus_tag="RHV13_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HDV1240059.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5351..5806)
                     /locus_tag="RHV13_000500"
     CDS             complement(5351..5806)
                     /locus_tag="RHV13_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HDV1240060.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6144..7346
                     /gene="ackA"
                     /locus_tag="RHV13_000501"
     CDS             6144..7346
                     /gene="ackA"
                     /locus_tag="RHV13_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDV1240061.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7421..9565
                     /gene="pta"
                     /locus_tag="RHV13_000502"
     CDS             7421..9565
                     /gene="pta"
                     /locus_tag="RHV13_000502"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDV1240062.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9755..11275
                     /gene="yfcC"
                     /locus_tag="RHV13_000503"
     CDS             9755..11275
                     /gene="yfcC"
                     /locus_tag="RHV13_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDV1240063.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11308..11850)
                     /gene="yfcD"
                     /locus_tag="RHV13_000504"
     CDS             complement(11308..11850)
                     /gene="yfcD"
                     /locus_tag="RHV13_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HDV1240064.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(11908..12459)
                     /gene="yfcE"
                     /locus_tag="RHV13_000505"
     CDS             complement(11908..12459)
                     /gene="yfcE"
                     /locus_tag="RHV13_000505"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HDV1240065.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12515..13159)
                     /gene="yfcF"
                     /locus_tag="RHV13_000506"
     CDS             complement(12515..13159)
                     /gene="yfcF"
                     /locus_tag="RHV13_000506"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HDV1240066.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLVLGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPECLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13295..13942
                     /gene="yfcG"
                     /locus_tag="RHV13_000507"
     CDS             13295..13942
                     /gene="yfcG"
                     /locus_tag="RHV13_000507"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HDV1240067.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATRQALLKAQLGDVRSDS"
     gene            13999..14361
                     /gene="folX"
                     /locus_tag="RHV13_000508"
     CDS             13999..14361
                     /gene="folX"
                     /locus_tag="RHV13_000508"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HDV1240068.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14382..15275
                     /locus_tag="RHV13_000509"
     CDS             14382..15275
                     /locus_tag="RHV13_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HDV1240069.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLDLGHKITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGQALHRPAILRVPATAIRLLMGESSILVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15323..16213)
                     /gene="rpnB"
                     /locus_tag="RHV13_000510"
     CDS             complement(15323..16213)
                     /gene="rpnB"
                     /locus_tag="RHV13_000510"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HDV1240070.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQYRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLEKGFD
                     HDVILTLTRLSPDDLIAQSH"
     gene            complement(16410..17183)
                     /gene="hisP"
                     /locus_tag="RHV13_000511"
     CDS             complement(16410..17183)
                     /gene="hisP"
                     /locus_tag="RHV13_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HDV1240071.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17191..17907)
                     /locus_tag="RHV13_000512"
     CDS             complement(17191..17907)
                     /locus_tag="RHV13_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV1240072.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(17904..18590)
                     /gene="hisQ"
                     /locus_tag="RHV13_000513"
     CDS             complement(17904..18590)
                     /gene="hisQ"
                     /locus_tag="RHV13_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HDV1240073.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18680..19462)
                     /gene="hisJ"
                     /locus_tag="RHV13_000514"
     CDS             complement(18680..19462)
                     /gene="hisJ"
                     /locus_tag="RHV13_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HDV1240074.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19683..20465)
                     /gene="argT"
                     /locus_tag="RHV13_000515"
     CDS             complement(19683..20465)
                     /gene="argT"
                     /locus_tag="RHV13_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HDV1240075.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20731..21300)
                     /gene="ubiX"
                     /locus_tag="RHV13_000516"
     CDS             complement(20731..21300)
                     /gene="ubiX"
                     /locus_tag="RHV13_000516"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HDV1240076.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21395..22912)
                     /gene="purF"
                     /locus_tag="RHV13_000517"
     CDS             complement(21395..22912)
                     /gene="purF"
                     /locus_tag="RHV13_000517"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDV1240077.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRIEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(22949..23437)
                     /gene="cvpA"
                     /locus_tag="RHV13_000518"
     CDS             complement(22949..23437)
                     /gene="cvpA"
                     /locus_tag="RHV13_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HDV1240078.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23752..24414)
                     /gene="dedD"
                     /locus_tag="RHV13_000519"
     CDS             complement(23752..24414)
                     /gene="dedD"
                     /locus_tag="RHV13_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HDV1240079.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24404..25672)
                     /gene="folC"
                     /locus_tag="RHV13_000520"
     CDS             complement(24404..25672)
                     /gene="folC"
                     /locus_tag="RHV13_000520"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDV1240080.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25742..26656)
                     /gene="accD"
                     /locus_tag="RHV13_000521"
     CDS             complement(25742..26656)
                     /gene="accD"
                     /locus_tag="RHV13_000521"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HDV1240081.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26812..27471)
                     /locus_tag="RHV13_000522"
     CDS             complement(26812..27471)
                     /locus_tag="RHV13_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDV1240082.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(27554..28366)
                     /gene="truA"
                     /locus_tag="RHV13_000523"
     CDS             complement(27554..28366)
                     /gene="truA"
                     /locus_tag="RHV13_000523"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDV1240083.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSRYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28366..29379)
                     /locus_tag="RHV13_000524"
     CDS             complement(28366..29379)
                     /locus_tag="RHV13_000524"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDV1240084.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29445..30581)
                     /gene="pdxB"
                     /locus_tag="RHV13_000525"
     CDS             complement(29445..30581)
                     /gene="pdxB"
                     /locus_tag="RHV13_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HDV1240085.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHERTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRNLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30680..31675
                     /gene="flk"
                     /locus_tag="RHV13_000526"
     CDS             30680..31675
                     /gene="flk"
                     /locus_tag="RHV13_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HDV1240086.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMTLKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31672..32850)
                     /locus_tag="RHV13_000527"
     CDS             complement(31672..32850)
                     /locus_tag="RHV13_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose transporter"
                     /protein_id="HDV1240087.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33134..34354)
                     /gene="fabB"
                     /locus_tag="RHV13_000528"
     CDS             complement(33134..34354)
                     /gene="fabB"
                     /locus_tag="RHV13_000528"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HDV1240088.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34513..36519
                     /gene="mnmC"
                     /locus_tag="RHV13_000529"
     CDS             34513..36519
                     /gene="mnmC"
                     /locus_tag="RHV13_000529"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HDV1240089.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEARFPEHLHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLSLAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPEELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQDLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSSLPVYSVAGQVSHIPTTPELAKLKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSDEDQQQNRQRLIDCFPQAQWAKEVDISEKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDKAVSAPVYDDLFMLAALGSRGLCSAP
                     LCSEILAAQMSEEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(36640..36918)
                     /locus_tag="RHV13_000530"
     CDS             complement(36640..36918)
                     /locus_tag="RHV13_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HDV1240090.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(36952..37500)
                     /gene="epmC"
                     /locus_tag="RHV13_000531"
     CDS             complement(36952..37500)
                     /gene="epmC"
                     /locus_tag="RHV13_000531"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HDV1240091.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTCLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARREQVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37500..38309)
                     /locus_tag="RHV13_000532"
     CDS             complement(37500..38309)
                     /locus_tag="RHV13_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDV1240092.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFIGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(38309..39133)
                     /gene="mepA"
                     /locus_tag="RHV13_000533"
     CDS             complement(38309..39133)
                     /gene="mepA"
                     /locus_tag="RHV13_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HDV1240093.1"
                     /translation="MNKTAIALLALLASSVSLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(39137..40222)
                     /gene="aroC"
                     /locus_tag="RHV13_000534"
     CDS             complement(39137..40222)
                     /gene="aroC"
                     /locus_tag="RHV13_000534"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDV1240094.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40257..41189)
                     /gene="prmB"
                     /locus_tag="RHV13_000535"
     CDS             complement(40257..41189)
                     /gene="prmB"
                     /locus_tag="RHV13_000535"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDV1240095.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPDAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREHFAIYKD"
     gene            41355..41906
                     /gene="smrB"
                     /locus_tag="RHV13_000536"
     CDS             41355..41906
                     /gene="smrB"
                     /locus_tag="RHV13_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HDV1240096.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKVSEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(42057..42899)
                     /locus_tag="RHV13_000537"
     CDS             complement(42057..42899)
                     /locus_tag="RHV13_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HDV1240097.1"
                     /translation="MKKWTFFLAALLLLIVNVGTSKSYAGSKLMSSTFKSTEIYYGMN
                     RISPVGDLFFYVTVLTPGEVSYGQYNSDARRGDILPVISWTGSGPAPQLKLTDYGRTD
                     TSNCPGINTKNFYCAYMTFDVTVESDNYGCPWQASFYSVSEQVGFGVYTSPTVHDSAC
                     PTIPVASFDISWSENYVSHNKALRLQSDGSTITTTLSTYLMESGKLCDGSVFDSRGAY
                     CRAVSDLLTFTSYGCDNAKVTVTPSRQPVTDKKLHDIVVQVNTSSRAPIDSTCRFQYV
                     LNEL"
     gene            complement(42902..43423)
                     /locus_tag="RHV13_000538"
     CDS             complement(42902..43423)
                     /locus_tag="RHV13_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV1240098.1"
                     /translation="MKKNSVLVLICSLLLFSGTVSAADNLHFTGNLFGKSCTPVINGN
                     LLAEIHFPTIAASDLMLRGQSERVPLVFQLKDCKSATAFNVKVTLTGTEDTDLPGFLS
                     IDSSSSATGVGIGIETAGGAAVPINSNTGATFPLNQGNNSVNFNTWLQAVNGRDVTSG
                     DFTATMTVTFEYF"
     gene            complement(43420..43890)
                     /locus_tag="RHV13_000539"
     CDS             complement(43420..43890)
                     /locus_tag="RHV13_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV1240099.1"
                     /translation="MKRISLLMLWGISSMALSNISFHGYLVQPPNCTISNGENIELTF
                     RDVNIDDINGSNYEQFVPYAITCDTTVRDPQMEMTLTWSGTQSDFDDAAVTTDINGLA
                     IHLKQAGSDFKLNTPLVVNETALPVITAVPVKKSGVDLPEADFEAWATLQVDYQ"
     gene            complement(43887..44387)
                     /locus_tag="RHV13_000540"
     CDS             complement(43887..44387)
                     /locus_tag="RHV13_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV1240100.1"
                     /translation="MKGSYSAWVWLLLCSIVSTPVMAGSKSVAMVLRVLVDAPPPCTV
                     TGASVEFGNVFISKIDGVSYKRPIDYSLVCNNLAMDDLRLNMQATTTVINGETVIDTG
                     IAGFGIRIQKASDHSILDLTPGTWLPFNFSSGAPALEAVPVVQSGVSLTAAEFSASAT
                     IVVDYQ"
     gene            complement(44398..45156)
                     /locus_tag="RHV13_000541"
     CDS             complement(44398..45156)
                     /locus_tag="RHV13_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDV1240101.1"
                     /translation="MTNLFCSARTAATTLALLITAASLSAQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKMPYLAQSWMEDEQGNKISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENVSMKNPWQHKV
                     TLTRSGQEFTVNNPTPYFVVISNASAQKNGNPAAGFSPLVISPKTSVPLKVKMDSVPV
                     LTYVNDYGARMPLFFTCTGNTCQVDEKQNSQGQG"
     gene            complement(45179..47818)
                     /locus_tag="RHV13_000542"
     CDS             complement(45179..47818)
                     /locus_tag="RHV13_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDV1240102.1"
                     /translation="MPDHSLFRLRVLPCCIALAMSGCYVNAWAEDEIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYASENDASKTYACLTPELV
                     AQFGLKEDVAKNLQWIHDGKCLKPGQLEGIDIKADLSQSALVISLPQAYLEYTDINWD
                     PPSRWDDGISGLIADYSITAQTRHEENGGDDSNEISGNGTVGVNLGAWRLRADWQTDY
                     QHSKSNDDDVINGDDTQKNWEWSRYYAWRALPSLKAKLGLGEDYLNSDIFDGFNYVGG
                     SISTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDINTASMPFLTRPGQVRYKLMMGRPQEWGHHVEGGFFSGG
                     EASWGIANGWSLYGGALADEHYQSAALGVGRDLSVFGAVAFDITHSHTRLDKETAYGK
                     GSLDGNSFRVSYSKDFDELNSRVTFAGYRFSEENFMTMSEYLDASDSEMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDRDEQTNYNVMLSHYFNLGSIRNMSISMTGYR
                     YEYDNQADKGVYISLSMPWGDSSTISYNGNYGSGSDSSQVGYFSRVDDATHYQLNVGT
                     SDKHSSVDGYYSHDGSLAQVDLSANYHEGQYTSAGISLQGGATLTAQGGALHRTQNMG
                     GTRLLIDADGVAGVPVEGNGAAVYTNMFGKTVVADVNNYYRNQAYIDLNNLPENAEAT
                     QSVVQATLTEGAIGYRKFSVISGQKAMAVLRLQDGSHPPFGAEVKNDNAQNVGLVDDD
                     GNVYLAGVKPGEHMIVSWGGVAHCDIHLPDPLPADLFNGLLLPCQQTGAISPSMPHQI
                     KPVIQEQTQQVMPTEAPVSVSAN"
     gene            complement(47900..48463)
                     /locus_tag="RHV13_000543"
     CDS             complement(47900..48463)
                     /locus_tag="RHV13_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV1240103.1"
                     /translation="MSKFVKTAIAATMVMGAFASTSTIAAGNNGTARFYGTIEDSPCS
                     IVPDDHKLEVDMGDIGSGILKNNGTSTPKAFQIHLQDCVFDTQTTMTTTFTGNASSTN
                     SGNYYTIYNTDTGAAFNNVSLAIGDAQGTSYKSGAGIDQKIVKDTATNKGKAKQTLDF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        11890 a        13072 c        12127 g        11399 t
ORIGIN      
        1 aaaaaaacca gacttacaat taagaatcag aacaattcac tatataacat tacatgtaaa
       61 gcatatacac ctcattattt tgtcattatt aagttattaa cagcacaatc gagccttccc
      121 ctctggcaaa atcttattct gcagaccttc aaaacaccgt cctgggggag tacattgttc
      181 taagctgact tccacggcag ggagtggcga taacagcaaa aaaggtcaag attcatgtcc
      241 cccattgaaa aatccagcaa attagagaat gtctgttatg acatccgtgg tccggtgctg
      301 aaagaagcaa aacgcctgga agaagaaggt aacaaggtac tgaaactgaa catcggcaac
      361 ccagccccgt tcggttttga cgcgccagat gaaatcctcg ttgacgtgat acgcaacctg
      421 cctacagctc aagggtattg cgattccaaa ggtctttact ccgcgcgtaa agccatcatg
      481 cagcactacc aggctcgtgg catgcgtgat gttaccgtgg aagatattta catcggcaat
      541 ggtgtatcgg agcttatcgt tcaggcaatg caggcattgc tgaacagcgg ggacgaaatg
      601 ttggttcctg caccagatta cccactatgg accgcggcgg tttcgctttc cagcggtaaa
      661 gcggtgcatt atctttgcga tgaatcctct gactggttcc cggacctcga tgatattcgc
      721 gctaaaatta cgcctcgtac gcgtgggatc gttattatca acccaaataa cccaaccggc
      781 gcggtatatt ccaaagagct tttaatggag attgtggaga ttgcacgtca gcataatctc
      841 attatcttcg ccgatgaaat ttatgacaaa attctctacg acgacgctga gcatcactca
      901 attgcgccgc tggcacctga cctgctgacc attaccttta atggactgtc gaaaacgtac
      961 cgcgttgcag gcttccgtca ggggtggatg gtgttgaacg ggccgaaaaa acacgccaaa
     1021 ggctacatcg aaggtctgga aatgctggct tcaatgcgcc tgtgtgctaa cgttcctgcg
     1081 caacacgcca ttcagaccgc gctgggtggt tatcagagca tcagtgaatt tattacccct
     1141 ggcggtcgtc tttatgagca gcgtaaccgc gcgtgggaat tgatcaacga tattccgggc
     1201 gtttcctgcg tgaaacctcg tggtgcgctg tacatgttcc cgaaaatcga cgccaaacgc
     1261 tttaacattc acgacgatca gaaaatggtg ctggatttcc tgttgcagga aaaagttctg
     1321 ttggtgcaag ggacggcatt caactggccg tggccggacc acttccgcat tgtcacgcta
     1381 ccgcgtgtcg atgatatcga gctgtctttg agtaagttcg cgcgtttcct ttctggttat
     1441 catcagctgt aatcttaatt tcactgccgg agattgcatc cggcagtgtt atcccgccac
     1501 aatgacctga tgatgtcatc atacgaaagg tcactatgaa acagagccat ttttttgccc
     1561 atctctcccg cctgaaactc attaaccgct ggccgctcat gcgcaacgtg cggacggaaa
     1621 atgtgtccga acacagtttg caggtagcga tggtcgccca tgcgctggca gctatcaaaa
     1681 atcgtaaatt tggcggtaat gtcaacgccg aacgtatcgc tttactggcg atgtaccacg
     1741 atgcttcaga agtgctcacc ggcgatctcc ctactccggt gaaatacttc aattcgcaaa
     1801 tcgctcagga atacaaggct attgaaaaaa tcgctcagca aaaactggtc gatatggttc
     1861 cggaagagct gcaggatatc tttgcgccgt taattgacga gcatgcatat agcgatgaag
     1921 aaaaatcgtt ggtgaaacag gcagatgcac tgtgtgcata tctgaaatgt ctggaagaac
     1981 tcgcggcggg aaataatgaa ttcttgctgg caaaaacgcg actggaagcg acgcttgaag
     2041 cgcgccgcag ccaggagatg gactacttca tggaagtatt tgttcccagc ttccatcttt
     2101 cgctcgatga gattagccag gattcaccgc tgtaagctgc cggagtctgc gtcgcatcag
     2161 gcaataagcg ccggatgcga catcaggctc ttgtcaaaac ggaaacagca tcgggatcat
     2221 caccacacaa accgccatca cgataatggt gaacggtacc cccaacttca caaagtcact
     2281 gaagctgtaa tttcccggac ctaaaaccag tgtgttaaca ggtgaagaaa ccggtgtcat
     2341 aaaggcggcg gatgctgcca tcgccacgac catcgcgaat ggataaggcg acacccccat
     2401 cgttttggca gcagccagcg caatcggagc catcaacacc gccgtcgcgg tattagagat
     2461 aaacagccca ataaccgccg acaagacaaa caaacaagcc agcatcatat gtggcccgta
     2521 accaccgcca atgtccatca gccctttcac cgccagcgcg acacctcccg ttttctgtaa
     2581 tgccacagca aacggcatca tcccaacgat caaaataatg ctcggccagt gaatggattt
     2641 ataggcgctt tcagcatcta tacagcggaa tttccccatc agcaggcagg cgatgatagc
     2701 ggcaacagga ttaggaattt catctgtcag cattaacgcc accattagca ccagacagaa
     2761 aatggcatgg ggtgcctggc tgtgcgcggg cgatgcttca ctcacctctt ccggtaagtt
     2821 cagcgctacg aagtcgcggc cctgtttggc cagcataccg atcagtttcc agttacccac
     2881 aaccaggatg atatcgccca gcagcagagg ctcatccgcc agcgaacctt ccagcgccac
     2941 gccattgcgc tttagcccca ccacattcag tccgtagcgg gtacgaaaac caatttcgcg
     3001 caccgattta ccaatcagtt ctgactcagg aattaatgaa atctctgcca tgcccacatc
     3061 aagggcctgg tcagaaaaat actcgccgcg cagtaccatc ggctccagca attgctcact
     3121 acaaaattgc cggagatcga catcagccgc agacatatca ataagcaaaa cgtcacgcgc
     3181 gcgaaattca gaaaccccat taacgttcac gataacgcga cgaaaacgcc gccagcgttc
     3241 aacaccgatg acgttagcgc cataacgctc acgtaatttg agatcatcca gccgttgacc
     3301 aatcattggc gatccggggc gaatagccag acgtcgcgca cgcccggtca gtcgatattc
     3361 acggataaga tcgcgaaagg ttcgacgcgt ccagccttcg cgctgcgggg tctgggtatc
     3421 ccctttcagc atgaaacgca tcactaacat atacaaaata cccagcacca gcacgaccag
     3481 gccaataggt gttacgctaa agaaactgaa gccgtgatag ccttcacgca gcaattcact
     3541 gttgactacc aagttcggcg gcgtcgccac cagcgtcatc atgccgctaa tcagcccggc
     3601 aaaacttaac ggcatcatca gacgtgacgg cgacgtttgc atacgcatgg caacgcttaa
     3661 caccacggga ataaagatag cgacaacgcc ggttgaactc ataaacgcgc caagccccgc
     3721 gacggtcagc atcaacaaaa ccaacatttt gatttcactg ttgcccgcaa ctttgaccag
     3781 ccatgttccc attacggtgg caacaccggt acggaccaaa ccatcgccaa taataaacaa
     3841 ggcggcaatc aggacaacgt taggatcaga aaagccggaa aatacttctg ggactgtcag
     3901 cgttccgctt aatgcaaacg cgacaataac aaacaaagcg accgcatcca tacgcactct
     3961 gcccgtcgca aacaagacga tggcaacggc cagtaatgaa agaacccaaa tcaattcacc
     4021 gttcacaact tatccttgtt aattgagggg gatgacttga ttctgccata aaaaagcccc
     4081 gacgagacgg ggctaaatca tgatcaggtg tttcactgaa taataacatc gccatttggc
     4141 tgtttggtca cagtaatttg ctccagactg tggaggacca aattgacctc attcaggcgc
     4201 ggggtatctg ccggagcgtt aaccgcaatg acatggcaac ccgccgccag gccagaaagc
     4261 acgccagcgg gagcatcttc caccaccaca cactcctgcg gcgcaagccc cagcagctgc
     4321 gcgcctaaca gatacgcatc aggttctggt tttccgcgct tcactcgctc agcggttaca
     4381 aacacctctg gtgcgggaag ccccgctatt ttatggcgcg ctcgcgctac cggcatggag
     4441 ccagaagtca caatggccca cggaatacct gctttattca aatgattgag taaggcgatt
     4501 gcccccggaa gcgcggtaat accttcggtt tccgtggcct cgatttgctc cagacgcgta
     4561 aactcggcgg caatatcagc ctcggatttg cccgccataa aatggcgcag agaggtgatc
     4621 gcctgtttac cgtgaatgaa agccagcacc tcttccggcg ctaacccatg acgtctggcc
     4681 cagttgctcc acgcccgttc taccgcaggc agggaatcca ccagcgttcc atcaagatca
     4741 aacagaaaac ctttgcaccg cacgcgggcc tcctcaggcg ttgataattt gattaatttc
     4801 gttggcgctc aaatggtact gacgcgggca ggcatgccac acattaagca tgcgctgata
     4861 tttttcccac attggcgtct gggcgttaaa accgtgagtt ccggcgtcaa aatgggtata
     4921 gcgcccttcc acattaacca taaagcggac ataaccgagg taacgtgctt cagtggcggc
     4981 gtcaaagccg aggaaggtga cacgacgttc atcgatggat tgctggtcct gcaaattaga
     5041 ccaggaaaca tgcaacgcat gatacatctc cataatgtcg atgatagtgc ggcaggtttc
     5101 ttctttcagc tcgccaaact cgcgatccag ttcacgcatc tgtaatccat aaccacgctc
     5161 aatgattgtt tgcaggcgac ggtaacgttc agcatttgcc ggatcgagca tagtcatcat
     5221 cttgtactga ttagacaaaa taagacgttg cgcgttagtc atttccattg ttgactcctg
     5281 tatcactcta ttacggtgaa aaaaaagaag gctgagtatg ccttctttta tatgcgtaat
     5341 caggggtcaa ttacaaatca tcaaggaaag ttttatccag ttgtttgaag gcgcgcttaa
     5401 gcgtgtcagc taatgcctgg taatcaggct tgccttcaac gggtgccaac acctgtccag
     5461 actcctgcaa tttaccgcga acttcataaa accagttgag gatagcaggg ggtaatggcg
     5521 ttacagaacg cttgcccagc caccacaatc cctgcatggg taaacttaag gcgaacagcg
     5581 cagtggcaac tgccggccca agctgaccgc ccagggcaat ctgccagcag agagtaaata
     5641 cggcgatcgg cggcataaaa cggatcgcat aacgcgtcat cttgataacg cgattttcga
     5701 caaagaccgg ggcaaggcgt ttttccagcg gccacgtctt tgagtaatgc tgtccccggc
     5761 gaaacaagct aaaaaaatta acagaacgat tatccggcgt tgacatgctt cacctcaact
     5821 tcacatataa agattcaaaa atttgtgcaa attcacaact cagcgggaca acgttcaaaa
     5881 cattttgtct tccataccca ctatcaggta tcctttagca gcctgaaggc ctaagtagta
     5941 catattcatt gagtcgtcaa attcatatac attatgccat tggctgaaaa ttacgcaaaa
     6001 tggcatagac tcaagatatt tcttccatca tgcaaaaaaa atttgcagtg catgatgtta
     6061 atcataaatg tcggtgtcat catgcgctac gctctatggc tccctgacgt ttttttagcc
     6121 acgtatcaat tataggtact tccatgtcga gtaagttagt actggttctg aactgcggta
     6181 gttcttcact gaaatttgcc atcatcgatg cagtaaatgg tgaagagtac ctttctggtt
     6241 tagccgaatg tttccacctg cccgaagcac gtatcaaatg gaaaatggac ggcaataaac
     6301 aggaagcggc tttaggtgca ggcgccgctc acagcgaagc gctcaacttt atcgttaata
     6361 ctattctggc acaaaaacca gaactgtctg cgcagctgac tgctatcggt caccgtatcg
     6421 tacacggcgg cgaaaagtat accagctccg tagtgatcga tgagtctgtt attcagggta
     6481 tcaaagatgc agcttctttt gcaccgctgc acaacccggc tcacctgatc ggtatcgaag
     6541 aagcgctgaa atctttccca cagctgaaag acaaaaacgt tgctgtattt gacaccgcgt
     6601 tccaccagac tatgccggaa gagtcttacc tctacgccct gccgtacaac ctgtacaaag
     6661 agcacggcat ccgtcgttac ggcgcgcacg gcaccagcca cttctatgta acccaggaag
     6721 cggcaaaaat gctgaacaaa ccggtagaag aactgaacat catcacctgc cacctgggca
     6781 acggtggttc cgtttctgct atccgcaacg gtaaatgcgt tgacacctct atgggcctga
     6841 ccccgctgga aggtctggtc atgggtaccc gttctggtga tatcgatccg gcgatcatct
     6901 tccacctgca cgacaccctg ggcatgagcg ttgacgcaat caacaaactg ctgaccaaag
     6961 agtctggcct gctgggtctg accgaagtga ccagcgactg ccgctatgtt gaagacaact
     7021 acgcgacgaa agaagacgcg aagcgcgcaa tggacgttta ctgccaccgc ctggcgaaat
     7081 acatcggtgc ctacactgcg ctgatggatg gtcgtttgga cgctgttgta ttcaccggtg
     7141 gtatcggtga aaatgccgcg atggttcgtg aactgtctct gggcaaactg ggcgtgctgg
     7201 gctttgaagt tgatcatgaa cgcaacctgg ctgcacgttt cggcaaatct ggtttcatca
     7261 acaaagaagg tacccgtcct gcggtggtta tcccaaccaa cgaagaactg gttatcgcgc
     7321 aagacgcgag ccgcctgact gcctgatttc acaccgccag ctcagctggc ggtgctgttt
     7381 tgtaacccgc caaatcggcg gtaacgaaag aggataaacc gtgtcccgta ttattatgct
     7441 gatccctacc ggaaccagcg tcggtctgac cagcgtcagc cttggcgtga tccgtgcaat
     7501 ggaacgcaaa ggcgttcgtc tgagcgtttt caaacctatc gctcagccgc gtaccggtgg
     7561 cgatgcgccc gatcagacta cgactatcgt gcgtgcgaac tcttccacca cgacggccgc
     7621 tgaaccgctg aaaatgagct acgttgaagg tctgctttcc agcaatcaga aagatgtgct
     7681 gatggaagag atcgtcgcaa actaccacgc taacaccaaa gacgctgaag tcgttctggt
     7741 tgaaggtctg gtcccgacac gtaagcacca gtttgcccag tctctgaact acgaaatcgc
     7801 taaaacgctg aatgcggaaa tcgtcttcgt tatgtctcag ggcactgaca ccccggaaca
     7861 gctgaaagag cgtatcgaac tgacccgcaa cagcttcggc ggtgccaaaa acaccaacat
     7921 caccggcgtt atcgttaaca agctgaacgc accggttgat gaacagggtc gtactcgccc
     7981 ggatctgtcc gagattttcg acgactcttc caaagctaaa gtaaacaatg ttgatccggc
     8041 gaagctgcaa gaatccagcc cgctgccggt tctcggcgct gtgccgtgga gctttgacct
     8101 gatcgcgact cgtgcgatcg atatggctcg ccacctgaat gcgaccatca tcaacgaagg
     8161 cgacatcaat actcgccgcg ttaaatccgt cactttctgc gcacgcagca ttccgcacat
     8221 gctggagcac ttccgtgccg gttctctgct ggtgacttcc gcagaccgtc ctgacgtgct
     8281 ggttgccgct tgcctggctg ccatgaacgg cgtagaaatc ggtgccctgc tgctgactgg
     8341 cggctacgaa atggacgcgc gcatttctaa actgtgcgaa cgtgctttcg ctaccggcct
     8401 gccggtattt atggtgaaca ccaacacctg gcagacttct ctgagcctgc agagcttcaa
     8461 cctggaagtt ccagttgacg atcacgagcg tatcgagaaa gttcaggaat acgttgctaa
     8521 ctacatcaac gctgactgga tcgaatctct gactgccact tctgagcgca gccgtcgtct
     8581 gtctccgcct gcgttccgtt atcagctgac tgaacttgcg cgcaaagcgg gcaaacgtat
     8641 cgtactgccg gaaggtgacg aaccgcgtac cgttaaagca gcagctatct gtgctgaacg
     8701 tggtatcgca acttgcgtac tgctgggtaa tccggcagag atcaaccgtg ttgcagcgtc
     8761 tcagggtgta gaactgggtg cagggattga aatcgttgat ccagaagtgg ttcgcgaaag
     8821 ctatgttggt cgtctggtcg aactgcgtaa gaacaaaggc atgaccgaaa ccgttgcccg
     8881 cgaacagctg gaagacaacg tggtgctcgg tacgctgatg ctggaacagg atgaagttga
     8941 tggtctggtt tccggtgctg ttcacactac cgcaaacacc atccgtccgc cgctgcagct
     9001 gatcaaaact gcaccgggca gctccctggt atcttccgtg ttcttcatgc tgctgccgga
     9061 acaggtttac gtttacggtg actgtgcgat caacccggat ccgaccgctg aacagctggc
     9121 agaaatcgcg attcagtccg ctgattccgc tgcggccttc ggtatcgaac cgcgcgttgc
     9181 tatgctctcc tactccaccg gtacttctgg tgcaggtagc gacgtagaaa aagttcgcga
     9241 agcaactcgt ctggcgcagg aaaaacgtcc tgacctgatg atcgacggtc cgctgcagta
     9301 cgacgctgcg gtaatggctg acgttgcgaa atccaaagcg ccgaactctc cggttgcagg
     9361 tcgcgctacc gtgttcatct tcccggatct gaacaccggt aacaccacct acaaagcggt
     9421 acagcgttct gccgacctga tctccatcgg gccgatgctg cagggtatgc gcaagccggt
     9481 taacgacctg tcccgtggcg cactggttga cgatatcgtc tacaccatcg cgctgactgc
     9541 gattcagtct gcacagcagc agtaacctcg tcatcatccg cagctttgcg ctgcggatat
     9601 ctgaaccgga aatagtcact atttccggtt ttttattctc ttaatttgca ttaatccttt
     9661 ctgattatct tgcttaactg cgctgcatca atgaattgcg ccatctcact ttgcatactt
     9721 accactttgt tttgtgcaag ggaatatttg cgctatgtcc gcaatcactg aatccaaacc
     9781 aacaagaaga tgggcaatgc ccgatacgtt ggtgattatc ttttttgttg ctattttaac
     9841 cagccttgcc acctgggtag ttccggtggg gatgtttgac agtcaggaag tgcagtatca
     9901 ggttgatggt caaacaaaaa cacgcaaagt cgtagatcca cactcatttc gcattctgac
     9961 taacgaagca ggcgaacctg agtatcaccg cgtacagctg ttcacgacgg gcgatgaacg
    10021 cccgggcctg atgaacttcc cgtttgaagg gttaacctca ggatcgaaat acgggacagc
    10081 cgttggcatc atcatgttta tgctggtgat tggcggcgcg tttggcattg tgatgcgtac
    10141 aggaaccatt gataacggta tcctggcgct tattcgccat acccgcggga atgaaattct
    10201 ctttattcct gcgctgttta ttctgttttc acttggcggc gcggtatttg gtatgggaga
    10261 agaggccgtc gcctttgcca ttatcatcgc accgctaatg gtccggctgg gctatgacag
    10321 tattaccacc gtcctggtga cctatattgc cacgcaaatc ggttttgcca gttcgtggat
    10381 gaacccgttt tgtgtggtcg ttgctcaggg gattgccggc gttccggtgc tttctggctc
    10441 cgggttgcgc atcgtggtgt gggttatcgc cactctgatt ggcctgatct ttaccatggt
    10501 ctacgcctca cgagtgaaaa agaatcctct tctgtcacgc gtgcatgagt ccgaccgctt
    10561 ctttcgtgaa aagcaggcgg atgttgaaca acgtccgttt acctttggtg actggctggt
    10621 attgattgtc ctgaccgccg taatggtctg ggtgatttgg ggcgtgatcg ttaatgcctg
    10681 gtttattcca gaaattgcca gccagttctt caccatgggt ctggtgattg gcatcatcgg
    10741 cgttgttttc cgccttaacg gcatgacggt taataccatg gcttcatcct ttaccgaagg
    10801 ggcgcgaatg atgatcgccc ctgccctgct ggtgggtttc gccaaaggga ttttgctgct
    10861 ggttggtaat ggtgaagcag gtgatgccag cgtgttaaat actatcctca acagcattgc
    10921 caatgccatt agcggtctgg acaacgcggt cgcggcctgg tttatgttgc tcttccaggc
    10981 ggtatttaat ttcttcgtga cgtccggttc tggtcaggcg gcgttaacca tgccgttact
    11041 ggcaccgctt ggcgatctgg tcggtgttaa ccgtcaggtt accgtgctgg cgttccagtt
    11101 tggtgatggc ttcagtcaca tcatttaccc gacctcagct tcgttaatgg cgacgctcgg
    11161 tgtttgccgg gtggacttcc gtaactggct gaaggtgggc gcgaccctgc ttggactgct
    11221 gtttattatg tccagcgtcg tggtgatcgg cgctcagttg atgggctacc actaaaaatg
    11281 ttaagagccg caattgcggc tcttttttca ttctgccgtt tcagtctcta cggcttcatt
    11341 tttggcattg cgcttcatcc acaacgctag cgctttcagc gagtctggag tgaactcatc
    11401 gcagcgtgcg gtgatttctt ccggcgtcag ccagcaaact tcactgactt catcttcctg
    11461 tagggcgaag ggaccgtgag agacgcagct gaacaatgcg ccccagacac ggcaattttt
    11521 atcttcgaaa tagaactgcc cgtgctcggc aaaggggaca ccggcaatgc ccaactcttc
    11581 ttccgcttcg cgacgcgcgg attccagcag ttgctcatcg gcctgaacca caccgcctgc
    11641 ggtcgcatct aacatgccgg gtaaaaagtc ttttgtctcg gtacgacgct ggaccagaat
    11701 tttgcccatg ccatcatgca cgacgatgta agttgcacga tgacgcagac actgtgcccg
    11761 catttgttcc cggctggctt gtgcaatgac ttcgttctct tcattgacaa tatccaccca
    11821 ttcagtactt gccaaacgac gctgttccat tatcaggaaa ccttcttttt ctggcgctct
    11881 tacggcgcat tttgagttgt gggtaaatta cggattaatc gcgacctgcg caatgatact
    11941 ttgatcattg agtgcgatta cactgagtac gtcattatcg agcatgccat aactcgccgg
    12001 attaccgcct ttcggaatac tcaccgagcc ggggttgaag tggaaaatct ccccccgctg
    12061 ttctgccact ggcagatggg tatggccgta caccagcaca tcgttctggt ttaaagcagg
    12121 cagattttct gggccaaaaa gatgaccatg cgtcaaaaac agacgttgtt tttccagtaa
    12181 tacctgttgc cacggcgcgg ttatcgggaa atgcagcagc atttgatcca cttcgctgtc
    12241 gcagttgccg cgcacagcga taaccttatg tgccacttca ttaagccgtt cagcgacttt
    12301 ggctggcgcg taaccctccg gtaaagcatt acgcgggccg tgattcaaca cgtcgccaag
    12361 gatcaccagc cactgggcac cgctttgggc aaacaactcc agaacacgtt ccgtcgccgg
    12421 taacgaccca tgaatgtccg atgcaaacat cagtttcatc actcactcct cgtcgaaaaa
    12481 acgtaagcta tgatactgga ttctgtcgtt gctatcagcc agattgcttc gctgaaagtg
    12541 caataaaacg ctggacagac gctcgctgcc actggaatgt cgcataatcc accaggcatt
    12601 ctggcacctc atcgccatgt agtaccaggc ggttaatcat tagcgccaga tcagtatcag
    12661 caatgcacca ttcaccaaat aaattcggct gacccagtac taacaaatgt tctgccatcg
    12721 cgaacagttt ctctgcactg gcttttccct cggccgttag tggcgctttt ttcgcccccg
    12781 caaagacaac atccgtcgga cgctcttcgc gaatgggcat cagatcgctg cgcagccagg
    12841 cctgaatctg tcgtgcacgc gcacgatttt ctaaatcaag cggataaata cgttcccagg
    12901 tcggtggcgc aaatcgatct tccagatact ccgcaatggc agaagattca ctcaactcaa
    12961 aatcatcgat ttgtaataac ggcacacggc gtgtctgacc gtaaccttgc cacgtcggtt
    13021 gcaaatgttc accgctgtcg aggtcgatgg tcttgatatg aaacgacagg cctttttcct
    13081 gcaacgccac ccaggcggat aacacataag gggagaaaaa atgggcatct gaccaaagcg
    13141 tgatagcggg tttactcatt atgtcctcgg caataaaggg ggctggctat caaactatag
    13201 agcctttttg tcactgtcac gtgacgaata ctcacgcaag tcgccgcgct ttctatactt
    13261 gtggtgttca gatgattaga caaccggagc tgcaatgatc gatctctatt tcgccccgac
    13321 acccaatggc cacaaaatta cgctgtttct cgaagaagca gggctggatt atcgcttgat
    13381 aaaggtagac ctggggaaag gcggtcagtt tcgcccggaa tttttgcgca tttcgcctaa
    13441 caacaaaatt ccggcaattg ttgatcattc tccagccgat ggcggcgaac cgctaagcct
    13501 ctttgaatct ggtgccattt tgttgtatct ggctgagaaa acggggctct ttttgagtca
    13561 tgaaacgcgt gaacgcgccg ccacattaca gtggttattc tggcaggtag gcggactggg
    13621 gccgatgctt gggcaaaatc atcattttaa tcacgcagcc ccgcaaacca ttccttacgc
    13681 tattgaacgt tatcaggttg aaactcagcg tctgtaccat gtactgaaca agcggctgga
    13741 aaactcgccc tggctgggag gcgagaacta cagcattgcg gatattgcct gctggccgtg
    13801 ggttaatgcc tggactcgcc agcgaataga cctcgctatg tatccggcag tcaagaactg
    13861 gcatgagcgg atccgttcgc gccctgccac caggcaggca ctgctaaaag cacaactcgg
    13921 tgatgtgcgt tcggatagtt aacagaaaca ggttctcgtg tattatttca tcctaagtaa
    13981 aacaacggag aacctgcaat ggcacaacct gccgctatta ttcgtataaa gaaccttcgt
    14041 ttacgtacgt ttatcggaat taaggaagaa gaaattaaca accgtcagga tattgttatc
    14101 aatgtgacga tccactaccc cgccgataaa gcgcgcacca gcgaagatat caacgatgcg
    14161 ctgaattatc gcaccgtaac gaaaaacatt attcagcatg tagagaataa ccgtttctct
    14221 ttgctggaaa aattaactca ggatgtgctc gatatcgcac gtgaacatca ctgggtgacg
    14281 tatgctgaag tggagatcga taaactgcac gcgctgcgct acgccgattc ggtatccatg
    14341 accttaagct ggcagcgtta accgccatat cgggaggctg catgaacata gtgatcaccg
    14401 gagggacggg attaattggt cgccatttga ttccgcgttt gctggatctg ggccataaaa
    14461 tcacggtagt gacgcgtaac ccgcagaaag ccagttccgt tctcggccct cgggtgacac
    14521 tatggcaagg gcttgccgat caaagcaacc tcaacggcgt tgatgcggta atcaacctgg
    14581 ccggagaacc gattgctgat aaacgctgga ctcacaagca aaaagagcgt ctctgccaaa
    14641 gccgctggaa tatcacgcaa aaactggtcg atttgattaa tgccagcgac acgccgccgt
    14701 cggtactcat ttccggctcg gcaacgggct attatggcga cttaggtgaa gtggtggtta
    14761 ccgaagagga accgccgcat aacgaattta cccataaact ctgcgcccgc tgggaagaaa
    14821 ttgcctgccg ggcgcaaagt gacaaaacgc gagtgtgcct gctgcgtacc ggtgtagtgc
    14881 tggcaccgga tggcggtatt ctcggtaaaa tgctgccgcc gtttcgtctt ggcctgggcg
    14941 ggccgattgg ctctggtcgg cagtatctgg cctggattca tattgatgat atggtcaacg
    15001 gcattctctg gctgctggat aacgagctgc gcgggccatt taatatggtt tcgccctacc
    15061 cggtacgcaa tgaacaattt gcccatgcgc tcggtcaagc gctgcatcgc ccggccattt
    15121 tgcgcgtccc cgcaaccgcc attcggctgt taatgggcga atcttcaata ctggtattag
    15181 gtggacaacg cgcgctgcct aaacggctgg aagaagcggg ttttgcgttt cgctggtacg
    15241 atttagaaga ggcgctggcg gatgtcgttc gctgatgtgg tttactgcaa acatccgcca
    15301 gttaactccc ggtgttacag ggttagtggc tttgcgcgat aagatcgtct ggtgaaagtc
    15361 gggtcagcgt caggatgaca tcgtggtcaa atcctttttc cagcatctcc tgagcaatac
    15421 gcagagcttc ttcgtgtttg ccctgcattg cgccttcttc acgtaatctg tcagcaatgg
    15481 tcatcagttt ctccttttct tgtggtgcgc gttctgttat ctcaccaata aatgcacgga
    15541 aacgctgggc atcccctgtt tgtaatacgt aattaaacag ggcttttagc tgtctgtcat
    15601 tagtgttccc tgtaactagc agcgaaacaa tttggtcgac taatcccaac agatcgcgct
    15661 gacgaatatg tttctgaatt aactccaaca gcgccatttt gcggtattgc ataatctcgt
    15721 catccggcac cacggtaata tccaccaacg gaaaagccga tgaatatatt ttacgggcaa
    15781 tcgcaggctc ggcaaattca tcaagccaac agagtgaata aggataagga cttctgcaac
    15841 catgataaaa cagcatcggg atcaccaatg gaagctcttt atagcccgca tcaagatggt
    15901 tttgcattgc cgcaatggaa taacgcatca tgcgaaaagc cattaattct tccggcttac
    15961 tttggtgctc tatcactaca taaatataac ccactccctc ctgcgttttc acagaccaca
    16021 agaggtcgga ataatattgc cgcaggtctt catcaataaa actgtttggt tccagtttaa
    16081 gcgtcgttaa atcacacagt ttgcgcagcg gcgcgggaag atgaatatca ataaaatccc
    16141 gcgcggtgtc tggatggcgt aaaaaagatt taaataccgc atcatgcggc gtggaagttg
    16201 tcgatattgt catggcgttc cgtcacccct caaatttaag atgacgcgac aataaccacg
    16261 attaacaccc aatgcagcag gcagttttta tctttggaca gcgaattcag aagtattttt
    16321 agcggactgc aacttttaca tttcagcgga agctgactcg caaattcccc ttgaaaatac
    16381 tccggcagcg ttagccaccg gagaggcaat tatttcagcg aacctttaag gaactgttgc
    16441 agacgagggc tttgcgggtt gccaaataac tgctccggcg cgccttcttc ctctattttc
    16501 ccctgatgga ggaaaatgac gtgagtagaa acatgacgag caaagcccat ttcgtgagtc
    16561 actaccacca tggttttccc ctcttctgcc agttgctgca taatgcgcaa cacttcgcct
    16621 accagttcag gatcgagcgc cgaggtaggt tcatcaaaca gtaaaacttc cggttccatc
    16681 gccagcgccc gcgcgataga aacacgctgt tgctgaccgc cggaaagatg caccggatat
    16741 ttcccctgcg cacgttcgtc tatcccgact ttcgccagat acttcaccgc ccgctcgcgc
    16801 gcttcctgct tgctcaggcc caacacctga atcggcgctt ccatgacgtt ttccagcacc
    16861 gtcatatggc tccagagatt gaagtgctgg aataccatcg tcaggcgtgt gcgcagtaag
    16921 cgcagttgat ttttatcggc gactttgagt tgaccgtctt tgtcgcgcac cagattgatc
    16981 gtctggccat tgaccacgat cgacccttca ctcggttttt cgaggaagtt aatgcagcgc
    17041 agaaaggtac ttttccccga tcccgacgat ccgatgatgc ttattacatc tccggcattc
    17101 gcttgcagtg agaccccttt cagtacttca tgttcgccgt agcgtttgtg caaatcgata
    17161 acgtttaatt tattctcgga catcgtattc tcagtgcgtt gaagaaggtt tcacatgctg
    17221 caaccagcgt ttttccgctc tgcgaaagag gctgatcagg acataagaga tgattaaata
    17281 gagcaccgcg gcaatgccga aggcggtaaa aggttgatac gtggcggcgt taatatcgcg
    17341 ggctattttc agcagatccg gcacagtggc cgtaaacgcc agcgcagtag agtgcagcat
    17401 caggatcact tcgttgctgt atgccggtaa cgcaatacgc agcgcagaag gcaaaataat
    17461 gcaacgatac attttaaaag tcgagaagcc ataggctctg gcggcttcaa tttccccatg
    17521 cggaaccgaa cggattgccc cagcaaaaat ctcggtagtg taagcgcagg tgttaagcgt
    17581 cagcgccagc acggtacagt tcaggccact gcggaagaaa gcgttaagga attcggttcc
    17641 cttaacaatc tcaagcgtgt acatgccgga atagaacacc agcaactgaa catacagcgg
    17701 cgtaccgcga aaaatatagg taaataacca gattggaaac tggatgtatt tattactgga
    17761 gacacgacca atcgccagaa acagcgccag gactccgcct atcactaccg ataaaataag
    17821 cagccacaga gtgatcgcca caccagtaaa gcgataaccg tcggtccaca gcagcggttt
    17881 ccagtattca tgtaagattt caatcacagg tcagccctct tcacacccac ggagtagcgg
    17941 cgctcaagga acagcagcac accattggaa acagtggtga aaaccaggta aatcacgcca
    18001 cagacgatgg cgaaatagaa cggttcccag gtacttttgc ctgccagttg ggtggctttg
    18061 accacatctt ccaggccgag taacgaaacc agtgcggtag atttgaggat cacctgccag
    18121 ttgttgccaa tgcctggcag ggcgtaacgc atcatcgccg gaaacatgat ccgccgaaac
    18181 acttgcccac gagtaaaacc gaacgccgtc gccgcctcta tatgcccttt cggcactgcc
    18241 ataaacgcgc cacggaacgt ttcggtaaaa taagcaccgt agataaaacc gagagtaata
    18301 ataccagcga ccatcggatc gatatcaatc tgcccgacgc ccatcgcctc cgtcaccgtg
    18361 tttagtgcaa tctgcaaacc gtagaaaatc agcagcatca acaccaagtc cggcacgcct
    18421 cgaatcagcg tggtgtaccc ttcgaaaatc agccccgaaa gccgattttg cgagagctta
    18481 ccgccagcgc caattaaacc gatgattaca gcgagcacta cagaactgat agccagctcc
    18541 agcgtgacga gcgcaccctg taaaataaca cctgaaaacc catacaacat gctgcctgtc
    18601 ctgtcgtgtg tggtgaatta ccgcctcttg tctccctccg gttacccgaa gggagcgggc
    18661 tttctttcac cgatggcgat taaccgccat aaacatcaaa atcgaagtac tttttcgcta
    18721 atttctcgta agtaccgtca gcgcgcattt cggcaaaggc tttgttcagt gcctcgcgca
    18781 gttcgttatc ttctttacgc aggcccatgc cggtccctac accaaacagt ttttcatctt
    18841 taacagacgg gccaccgaat ttgtaatctt taccgacggg ttgtttgagg aaaccttcac
    18901 tggcagcgac ctcatcctgg aacgcggcat caatacgtcc ggcagtcagg tcagaataaa
    18961 tgttgtcctg cccctgatac gagacgattt caatgccttt tggtgcccag tgttcgttac
    19021 cgaacgtctc ctgggtggtg ccctgcaata cgcctacccg cttgcctttc agcgactcga
    19081 ctgtcggctg aatgtcggaa tttttcgcca ccaccagacg agaatcggca gcgtacagtt
    19141 tgtcggtgaa ggcaatttct tgctgacgtt tttccgtaat ggaaagcgat gacatgatgg
    19201 cgtcaatctt cttcgctttt aaggacggga ttaacgcatc cagcggattt tcgacaaacg
    19261 tacattgcgt attgatgcgt ttgcataatt cctttgccag atcgatatcg aagccaacca
    19321 gttcgccttg tgaatttttt gattcaaatg gcgcataggt cgggtcggta ccgatgcgga
    19381 tgttttgcgg aatcgcagca aatgccgcag ttgcgctgga gaaggccaga accagagaga
    19441 gcgatagcac cagttttttc ataatgtcct caactgacag tctttttata gggaatattt
    19501 acaggtttgt tacacttatc gtgccataaa attgaccatc aaggcaaaat atcccgcccc
    19561 gttagacgta ttatttttca ttaaaaatgc ttaagtatgc atttcaatgc accacgatag
    19621 tgcaacaaac ctggcgtgca ccatgatgat cattccatca ggtacagctt cccagcgacg
    19681 tatcagtcac cgtagacatt aaagtcgaaa tactttttcg ccatcttgtc gtaggtgccg
    19741 tcctgacgca gctcgccaag cgccttattg aaggcagccg tcagttcagc atcatcttta
    19801 cgtagcccta caccggtgcc atcaccgaag tattttttgt cttttactga tgagccagca
    19861 aaggcgaaat ctttaccagc aggttgcttg aggaatcctt cgctggcagc aacttcatct
    19921 tgtaacgcag catccagacg tcctgcagcc agatcggaat agaccaaatc ctggttggca
    19981 taggccacca catccacgcc tttgctacgc caggtctcgt tagcgtaagc ttcctgggtt
    20041 gatccctgca gcacaccaac atgtttacct ttcagtgaat ccagcgttgg ctgaatcggt
    20101 gaacctttgg cagcaatcaa acgagaatct gcggcgtaca gcttgtcgga gaaggcaatc
    20161 tcctgctgac gtttatcggt aatggaaagc gacgaaataa tggcgtcgat ttttttcgct
    20221 ttcagtgagg ggatcagcgc gtcaaagtca ctggcaaccc aggtacattt cacctgcatc
    20281 cgtttgcaca tctcgttacc gagatcgata tcaaagccaa caaaatcacc tttagcatct
    20341 ttcgacgaga acggcgcata ggtggtatcg gttccgatac gtaccgtctc cggtagcgcc
    20401 gcatagctgg aagccgctgc ggagagaccg actaacaaag acagagcgag aatcgacttc
    20461 ttcatacata accctcaagt gaaaatggct tttttatgtt gtgtattgtg ttgtgtgttt
    20521 gcaggctctt tcatgcaggt cttatgccat cttgacgaca acagtaacat tcaacgttaa
    20581 atatgttaat aagacgttgc attattgtcc tgaagttgaa gatagcaggt atagcggttg
    20641 aatcgcagcg tttcgattga atggcagaaa caaaatgtcg aggatttgat cgcggttgca
    20701 aaattgccct gaaacagggc aacagcggag ttatgcgccc tgccagcggg caaagagatc
    20761 ttcaggaagg gttattgcaa actggtcaag aacacgatta accgtctgat ttatcacatc
    20821 atcaagggat tgcgggcgat gataaaacgc cggaacggga ggcataatca ccgcaccgat
    20881 ttctgccgcc tgagtcatta aacgcagatg gcctaagtgc aatggtgttt cacgcacgca
    20941 gagcaccaac gggcgacgct ctttcagcac cacatctgcc gcacgggtca gtaagccatc
    21001 agtatagcta tggacaatgc cggaaagggt tttgattgaa cagggtaaaa tcaccatccc
    21061 cagcgtctgg aaagaaccgg aagagatgct ggcggaaata tcgcgcgcat cgtgcgtgac
    21121 atcagctaat gcctgcactt cgcgcaggga aaaatccgtt tcgagggata aggtctggcg
    21181 cgccgcctgg ctcatcacca gatgcgtttc gatatctgtg acatcgcgca gaacctgtaa
    21241 taagcgcacg ccataaatcg cgccgctggc accgctgatg cctacaatga gtcgtttcat
    21301 aaatagttgc cctgtcagac ttgcaggcag actttgcagt atttcggcgg gagttgcaag
    21361 tcagggtgcc agaccggcac cctgagcgaa ggcatcatcc ttcgttatgc atttcgagat
    21421 tttccacttc gttctgacgt tgcactgctt tggcgtcatc attacgtaac gtatcgagga
    21481 aatcgaggta gccctgatca acatctttgg tgacgtagac gccgttgaac accgagcatt
    21541 caaactgctg gatatcagga ttttcagcgc gaacggcgtc gatcagatcg ttcagatcct
    21601 ggaaaatcaa cccgtcagca ccgatgatct ggcgaatttc atcaacttcg cgaccgtgag
    21661 cgatcagttc cgtggcgctc ggcatatcaa taccataaac gttcgggaag cgaatttccg
    21721 gtgccgcaga agcgaggtac actttcttcg ctccggcttc gcgtgccatc tcgataatct
    21781 gctcagaagt ggtgccacgg acgatggagt cgtcgaccag caagacgttt ttatcgcgga
    21841 actcggcgcg gttggcgttc agtttacggc gcacggactt acgacgcagc tgctggcccg
    21901 gcatgatgaa ggtgcggcca acatagcggt ttttaacgaa gccctggcgg tacggtttgc
    21961 ccagaatacg agcaatttcc agcgcgatat cacacgaggt ttccgggatc gggatcacca
    22021 cgtcgatatc cagatcttcc cattcgcggg caattttctc gcccagtttc gtgcccatat
    22081 tcacacgcgc gctgtaaacg gaaattttgt cgatgaacga gtccgggcga gcaaagtata
    22141 catactcaaa caggcacgga ttgctgaccg gattgtcagc acattgacgg gtaaacaact
    22201 gcccttcttc agtgatgtaa atcgcttcgc caggcgcgac atcacgcagg aaatcaaagc
    22261 ccagtgtatc gagcgcaacg ctttcggaag cgaccatata ttctatgcgg ttctcgtcaa
    22321 tatcacgttt tcccagtacg agcggacgaa taccgttagg atcgcggaaa gcaaccatac
    22381 cgtggccgat aatcatcgcc acacaggcat acgcgccgcg gattaagcgg tttgtagcag
    22441 cgatggcggc gaaaatgttg tcggcttcca gcgggtagtg gcggaagttg tccagctcgc
    22501 tggcgaagat attaagcaga atttccgagt cggaagtggt gttgatgtgg cggcgttttt
    22561 cttcaaacag ttttttacgc aactcgtgag cgttggtcag attgccgttg tgggcaagcg
    22621 taatgccata cggagagtta acgtaaaacg gctgcgcttc agaggcgctg gagctgccag
    22681 ccgtagggta acgcacatga ccaatgccca tattgccctg caaacgctgc atatggcgag
    22741 cttcaaatac atcgctcacc agcccgttcg ctttacgcaa acggaagcag ttattggcat
    22801 ctatggtgat gatgccggcg gcatcctgac cgcgatgctg aagcaccgtt aaggcatcat
    22861 aaatcgactg gttaaccggc ataacaccgg cgataccgac aataccgcac atacgtcttt
    22921 ttcctcgtta agccacatct cagagcactt acgctctggg caagaaactt gacgagcttt
    22981 gcagataatc aaaaaaccat ctgatgataa aactgaactg cgggatcagc tgtgatttgc
    23041 tccagtcttc gcttttcgac accccggtaa aggagtcgag aaagaagaga atggcagcaa
    23101 caatcaacac accgcgcaac gcaccgaaac agacgcccag cacccgatcg gtgcctgaca
    23161 accccgtttt ctccaccagc tggcctatca cgaagttcac gatagcacca acgatcaggg
    23221 tagcgataaa cagtaccgcg atggcaatcc catttcgaac cagttcgtct tcaaagcccg
    23281 taaaccagac tgacaggtaa gtgtagtaat gactggcaac aaagaaagca cacccccatg
    23341 tcaccagcga taacgcttca cgaacaaagc cgcggatcag gctaaccaga gaggaaaaag
    23401 caatcaccgc gattatggcg taatcaatcc agaccatatg tgtcccacga ttttacgccc
    23461 tgtcatcctg ttcggggcgc attctaacag aaaaagaaaa cgtttgcgta gggatttcct
    23521 tcccgcgcat caataaaaat ggcgctgaaa aaatattcaa cgccatcgac tttttatgcc
    23581 tttgcggcat cgtgcaatgc gtgtcggatg cggcgtaaac gccttatccg acctacggtt
    23641 ctacccctgc gtaggcctga taagacgcac cagcgttgca tcaggcaacg gctgtcggat
    23701 gcggcgtgaa cgccttatcc gacctacggt tctacccctg cgtagaccgt attaattcgg
    23761 cgtatagccc attaccacgc cacttaagcc agaaagttgc ttcaactcac ccagcgatcc
    23821 cttcagctta tctttcgagg catctggccc aaccagaata cgggtaattt taccctgcac
    23881 tggcgtggat ggcgacgtat aaacccgata accggcaccg cgcagcttac cgacaatctc
    23941 attcacttta tcggcatttt tcagcgcccc cagttgcaca acataggctt tacccgtcgg
    24001 tgcagctttt tcttccacga ccggcttcgg ttctggcttc ggcgcaggtg gtgcttcaac
    24061 cttcggttta ggcggctcca ccggtttcgg cttcggtggg gcgaccggtg caggttccgg
    24121 ttcaaactcg gtgttattgg ctgcaatagt ggccggatcg agcgacggtg ctgcggcatc
    24181 acctgcccgc acctcttccg ctgcgccttc cggcggctgc gtcggcaacg cctgggtggc
    24241 ggctggcatc atatcaggct catcgcgatc gcccgctttt ggaaccagcg ggatagccgc
    24301 aaactcatcc tgataatgtt ttttctgccc gtccagcagc cctggaagta caatcacccc
    24361 cagcgccacc agcacgatcg tgcccactaa ccgattctga aacttacttg ccaccgcttc
    24421 tcctcgcgtc aatcacttcc atgacatgtg cgaccgtgtg gaaagaacca cacaccagca
    24481 cggtgtcttc cgctttagcg tccgccattg cggcatccca tgcctgcgca acgctatcaa
    24541 atgatttgcc gttacccaaa tgctcaagca gttgttctgc cgtggcaccg cgcggccctt
    24601 ccagtggcgc acaataccag tcatcaacca cgcttttcaa ccaggccaga gttccggcaa
    24661 tatctttatc atgtagcata ccgataaccg ccagcacgcg cccgtttttc ggtagcgctt
    24721 tcatacgccc ggtgagatat tccgccgcat gtggattatg cgcgacatca aaaataacgc
    24781 gtggcgactc gctcacaatc tggaaacgtc ccggcaaaat tgcgctggca atcccgtcgc
    24841 gaatggcatt ttcactgacc tccagcccgc tggcacgcag tgccgccagc gccgttgcgg
    24901 cattcggttg cgggacaagc ggcaacggca ggttttccag cgtgccgtga gcatcgctaa
    24961 acgcccagtc atgatcggtg acggaatagt tccactcaac gccccgacgt tgtaacagtg
    25021 cacctttttc ctgcgccaca tcagcaatgg tagaaggcat ttccggctca ccgacaattg
    25081 ccggtttttc gctgcggaag atgcctgctt tctcgcgacc aatactttcg cgatctggac
    25141 ccagccagtc ggtatgatcc agcgcaatac tggttactac cgcgacatcg gcatcgacaa
    25201 tattggttgc gtccagacga ccgcccagcc ctacttccag aatcaccacg tcaagttgtg
    25261 cctgcttgaa cagccacaac gccgacagcg taccgtactc gaaataggtc agggaaatat
    25321 caccgcgtgc cgattcaatc tccgcaaaag aggcggtgtg ggccgattcc ggcaattcct
    25381 ggccctgcac acgtacgcgc tcggtataac gcaccagatg aggcgaactg tagacgccca
    25441 ctttgtaccc tgccgccatc agaatcgact ccagcgtacg gcaggtggtg cctttgccat
    25501 tcgtacccgc aacggtaaac acaaatggcg ctggtttcag gacgccaaga cgcgccgcga
    25561 cctggctcac gcgctcaagg ccgagatcga tagttttact gtgcaggttt tccagataag
    25621 aaagccacga agccagaggc gacgcggctt gaggagtgcg tttgataatc atggtatccg
    25681 ctgattcgtt acggtgagaa tagcaaaagg gcagagccag tggccctgcc cttatcagtt
    25741 atcaggcctc aggttcctga tccggtaccg ggggtaccac tacgccttca cgcggcgctt
    25801 caggattcgg cgctggcaga ttcatcaact tcgccagaat gctcgccagt ttcaggcgca
    25861 tttccggacg acggacgatc atgtcgatgg cgcctttctc gatcaggaat tcactgcgct
    25921 ggaatcctgg cggcagtttt tcgcgaacgg tctgttcgat aacacgcgga ccggcaaagc
    25981 caattaacgc tttcggttca gcgatgttga gatcgcccag catggcgaaa cttgcagaaa
    26041 caccgcccat cgtcgggtcg gtcagcacgg agatgtacgg caagccgcgc tcctgcattt
    26101 ttgccagtgc cgcagaggtt ttcgccatct gcatcagcga catcagtgct tcctgcatac
    26161 gtgcgccacc agaggcggag aagcagatca gcgggcagtt atcttccagc gcctgctcaa
    26221 cggcacgcac gaaacgtgca cccacaacag accccattga accgcccata aaggcgaact
    26281 cgaatgccgc agcgacaacc ggcattccat acagagtgcc tttcatcacc accagcgcat
    26341 ctttttcgcc ggtttctttc tgcgcagatg ccagacggtc tttatacttc ttggagtcac
    26401 gaaacttcag cacatctttc ggctcaagct cgctacccag ctccacaagg cttccttcat
    26461 ctaacaggct atgcaggcga ttacgcgctg tcatacgcat gtgatggtca cacttcggac
    26521 agacctcaag attacgttcc agctcagcgc ggtataaaac ctgaccgcag ctatcacact
    26581 tagtccacac cccttcagga atgctcgcct tgcgggtggg agtaatgttg cttttaattc
    26641 gttcaatcca gctcattagg gacctttctg tctgaacctg gttcgatgcc agttttatct
    26701 ttggggacgc ataatgccat ttttgccccc aacagaccat gaatgttgca cattaaaaca
    26761 taacagcccg aaactttgga taaaaaagtg gtcgaaccgc ggagttactt tttattttgc
    26821 ggcgcgtgcc gcagcgcgtt tgtgacggat tatttcgatg acacccggca atatagaaac
    26881 cacaataatc ccgacgatca gcagcttaag gttatcctga accatcggga ttgtaccgaa
    26941 gaaatagcct gcgtaggtaa aaagcagtac ccacaacagt gcgccgatca cgttataagc
    27001 ggcgaaatga cggtacgaca tgtggcccat tcccgcaaca aacggggcga acgttctgac
    27061 gatcggcaca aaacgggcga gaataatcgt tttgccgcca tgtttctcat aaaactgatg
    27121 ggttttgtcg agataactgc gacggaaaat tttcgaattg ggattactga acaacttttc
    27181 accgaacaac cgcccgatag tgtaattgac cgcgtcaccc acaatcgcgg cgatcagcat
    27241 cagcaccacc atcatatgga cgttgagatc gttggtttcc agtgatgcca acgctccggc
    27301 aacaaacagc aatgaatcac ccggtaaaaa cggcgttacc accagaccgg tttcacagaa
    27361 caaaattaag aacaaaatgg cataaaccca gacgccgtac tccgcgacca gctctgccag
    27421 atgcacgtca atgtgcagga taaaatcgat gaggaaataa atcaggtcca tatttgccta
    27481 tgccttgtac tcgtcatctt tcaggctgta actacgttgg ctacagcctg aaatgttacg
    27541 agcgttattc tcgttagtcc gccagaaata gcgggcccat tggcggtttt ggaagatcat
    27601 accggtcagg gtaatccacc gcgaccagat acagcccttc cgcttttgcc gttgctgccg
    27661 ccagcgttct gtcctttgcc gccagcaact ctgctatcca gctctccggc tggttgtggg
    27721 caccgacttc catcaggctg ccgacaatat tcctgaccat atgatgtaca aaggcattcg
    27781 ctttgatatc taccaccaca taaggaccgt gacgcgtgac attaatatgc ataacgttgc
    27841 gccacggggt tcgggactgg cactgcaccg cacggaacga ggtgaaatca ttctcgccca
    27901 gcaagcattg cgcagcccga tgcatccgtt cagcgtccag cggttcgtaa aaatgggtta
    27961 cccctttact cagtaccgcc gggcgcagcc gatgattgta gatgatgtag cgataacggc
    28021 gagccgtggc gctaaatcgg gcatgaaaat catcaggtac agctttaacc caacgcacgg
    28081 cgatgtcacc aggtaaattc gcatttacgc ccaacgtcca cgcagcgtct ttgcgctgcg
    28141 cggtagtttc gaaatgcacc acctgcccgg taccgtgtac accggcgtca gtacgcccgg
    28201 cgcagaagac ggtgatgggt tcgttcgcca cctgagagag cgccttttcc agcttctcct
    28261 gcacactgcg gacttcattc tgccgttgcc agccgtaata cctactgccg tcgtactcaa
    28321 tgcccagcgc aattttataa actggcggtt gttgctggtc ggacattagt acagatactc
    28381 ctgcaccagt ttctcggcga tttttactgc catcagcgcg ccgccaaagc gaacgttatc
    28441 ggccaccgac cagaactgga cttgctccgg cataccgtag tcattacgca cgcagccaac
    28501 agaaagatgc ggcgtaccag aagcatcacc tacctgagtt gggaattcgt tctcttcaga
    28561 gagcacaata tcttcgcctt gagcaaacgc atcacgcgct tcttctgccg ccagcggacg
    28621 cagtgcttca aagttgacca tctgggcatg accgtagaat accggtgcct ggacgacgct
    28681 tgccgaaatc atcagtcctt cgtcctgcag gattttgcgt acttcgtcaa cgatacgacg
    28741 ttcttcacgc acgctacctt cgctatccgg cagtaacggc agcatgttga acgccagctg
    28801 acgcccgaag aaatcttctt cgtcaatcgg aatgccgttg agcaatttag cactctgccc
    28861 cgctaacgca tcgaccgctt ttttgccctg ggcggaggct gaaatcaggc tggtaacgct
    28921 gatacgtgat aaaccgccct gatcgattaa cggtttcagt gccgccagca gctggctggt
    28981 cagactgtcc ggtacggcga tgacattccg gttgcggtaa tcggtcagta caaacgggtt
    29041 tacttccggc accaccagcg gtacgtcggg ttcgagagca aacaatccgc tgctgtcgat
    29101 caccaggcaa cctgagttgg tcgcttcttc aacccaggct gcggtagctt ctttgcctgc
    29161 gacaaaaaat gccagctgcg cctgcgtcca gtcgaattca gcagcatcct gcacggtgat
    29221 tgtcttacca ccaaagcgca gttgttcgcc tgcgctttcg ttacgtgcca gtgcataaat
    29281 ttccccaacc gggaactgac gttcagccag cgtttcaagc agggcttcgc ccacagcgcc
    29341 agttgcgccc aggacggcaa tgttccagcc ttcagacatg gtggtttact ccagaaatag
    29401 caaagctccc tgccaatgtt acagcagaga gcatgaagaa gagattaacg tgccggatga
    29461 tgaacggcgt taaaacccag tttacacagc aatgatgccg cactggcgtc atcacaaatt
    29521 acatacagag atgaccattc acggcgctca agatagtttt tgcgcagttt atcgaactca
    29581 cccggtatcc cggcgacttt acgcagcggt gcgtcatcgc ggcgcacatc atacaccaaa
    29641 tgcaccagcc ttttcagcgt cggttgatcg agcgggccat gcagcgtaat gcgaccaaac
    29701 tctggcgcgg gcagtaatgt atccagcgca acgtgctgtt catgcccaat aaacttgcta
    29761 taggcttcaa acacttgcgt agtaccgcgt gctttacctt ccagggtata gcctgcaata
    29821 tgcggcgtgc cgatatccac ttttgtcagc aattccacat ttagttccgg ctcgccttcc
    29881 cagacatcca gcaccacgct taacttctgg ccttcattca ggcaggtcag cagcgcggta
    29941 ttatcgacga ctgcgccacg gcaggcgtta atcagaatcg ctccgggctt caggttacgg
    30001 atcagttttt catccgccag atgcagcgtt ttgtacggcc cgtctttgaa gagtggcgta
    30061 tggaaagtca gaatatccgc atgctgaact aattcatcca gcgagcggaa atcaccctca
    30121 tccccacggt cggcgcgagg cggatcgcaa agtaaggttt taatccctaa cgcttccagt
    30181 cgtgcctgta aacgtcgacc aacgttacca acgcctacga tccccacggt acgctcgtgc
    30241 agtgaaaatc catcgcgttc ggcaagcatc agcagggagg agaaaacata ttccaccacc
    30301 gcaatcgcat tacagccagg tgcagcggaa aaaccaattc ccgcctgctt caaccatgct
    30361 tcatcgacat ggtcggtccc cgcagtggca gtgccaacaa atttaatggg ttttcctgcc
    30421 agcaaagatt cattcacttt cgtgaccgaa cgcaccatca gcgcatccgc gtctgccagt
    30481 tgagcgacgg ggattggacg cccgggaacc gcggtcacct cacccaaacg gctaaataag
    30541 tcgcgggcat aaggcatatt ttcatcaaca aggattttca cgtttgtgtt acctgtatga
    30601 gacgagagtt aaccggacaa gtgtgccata atctcgcggc caggcatact tgcgaagatt
    30661 tcaggtataa ggatacgtaa tgatacaacc tatttccggc cctcctcctg ggcaaccacc
    30721 aggtcaggga gataacctgc cgtctggcgc gggcaatcag cctttatcca gtcagcaacg
    30781 tacttcgctg gaaagcttaa tgacgaaagt gacctcactg acgcaacagc aaagagcaga
    30841 actgtgggcg ggtatcaggc acgatattgg tctgtcggga gattcaccgc tgctttcgcg
    30901 tcacttccct gccgctgagc ataatctggc gcaacgtctg ctggccgcgc aaaaaagcca
    30961 ttctgcccgc cagcttttag ctcaattagg ggagtattta cgtctgggga ataatcgtca
    31021 ggcggtcacg gattatatcc gtcataactt tggtcagacg ccgctgaatc agctctcacc
    31081 ggagcaatta aaaaccattc tcaccctgtt gcaggaaggg aagatggtta ttccgcaacc
    31141 gcagcagcgc gaggcgaccg atcgtcctct attaccggcg gagcacaatg cgctcaaaca
    31201 gctggtgacc aaacttgcgg cggcaacggg ggaacccagc aaacagatct ggcaatcgat
    31261 gctggaactt tccggggtga aagatggtga gttaattcca gcgaaactgt ttaaccatct
    31321 ggtgacctgg ctgcaggcgc gtcagacgct aagccagcaa aatacgccga cgctggaatc
    31381 actacagatg acgctaaaac aacctttaga tgccagtgaa ctggcggcgt tatcggcata
    31441 tatccagcaa aaatatggcc tttctgcgca atcatcgctt tcttctgccc aggccgagga
    31501 tattcttaat cagctttatc aacggcgggt taaagggatt gatccgcgtg atatgcaacc
    31561 gctgcttaat ccttttccac cgatgatgga cacgttgcaa aatatggcaa cgcgtcccgc
    31621 gctgtggata ctgttagtcg cgattatcct gatgctggtc tggctggttc gttaaccccg
    31681 acgaaatgac agtatcgtga caataattcc cagcaccgca gagatcgccc cggcaagaaa
    31741 taccgaagag taaccaaacg tggtcgccag cattcccgcc agcggcccgg agacgccgag
    31801 ggcgatatcc tgaaacgcgg cgtaaccgcc cagtgcggtg ccgcgaactt gtgaggggac
    31861 acgtttaacc acctccacgc ccagcgcagg aaagataagc gaacatccgg ctccggttaa
    31921 cgccgcgccc gctaatgcga cccatgcacc aggggcttgc cagagcagca acaagcccac
    31981 cgtttctaca agcagagaga cgatcgccac tttcacgccg ccaaaacggt ccggcatcca
    32041 gccaaacatg acgcgcatca cgacaaatgc gccgccaaac gcggtaagag taaagcccgc
    32101 catcgcccat cctttgctgg caaagtagag cgaaacgaaa gtcccgataa ccgcaaaacc
    32161 aacgccttgt agtgccagac ctaaccctgg tttccagata agcccaacaa cgctccacag
    32221 cgatggacgt tctcccgcca gggccggtac tttgcgcact gtgccgttac aggcccacgc
    32281 cagtaagggt aataccattg tggtgatcgc cagtgcggca aaaccgtaat ggctatgaat
    32341 caacaggcca agcggagcac caacagcgag ggcaccgtaa atcgccattc cattccatga
    32401 catcactttg ccagagtgtt ttggccctac gatgcctaac ccccaggtca gagcgcctgt
    32461 cagtaactgg ctttcaccaa agccaagaat caaacgcccg atgaccaaca gggcaaattt
    32521 gaacggtgcg gagacaggca aaatcgccgc cagcagcaac gcgccgcccg ccagaccaca
    32581 agctaacatc ccctgaagcg ccgaacgttt tgcgccatat tgatcggcca gtcgcccggc
    32641 atagccacgc gtcagcaccg tagccagaaa ctgaatcccg acggcaatgc cgaccatggt
    32701 attgccatag cccagttcat gatgaacaaa cagcgggata accggcaacg gcaaccctac
    32761 ggtcatgtag gtgagaaaaa ccgcaaaagc gatgcggaag agcgaaaaat tggcagaaga
    32821 tcgtgtttcg gtttggctta cagcagtcat gcattactcc agaatgcagc gcaaggcgag
    32881 gagtatcccc gtctcatctc tctggtttca gggttacggt gcgttggcag gaattaacgc
    32941 gtacgtcttt tcagaaggaa atcgacaaag cgggaagttt gcctggaaca ggcggcgatt
    33001 gtcaatgatg tgaaaaaggg aaccatcggg ttccctttgg cgttagtgcc ggaggccgca
    33061 ttggcgcgta acgtcggatg cgacgctggc gcgtcttatc cgacctactc cgactactcc
    33121 gacctactgc gaattaatct ttcagcttgc gcattaccag cgtggcgttg gtgccgccga
    33181 agccgaagct gttagacata acagtggtca gttcgcgatc ggtcgtttcg gtcacgatgt
    33241 tcagacccgc agcctgctcg tccagctctt caatgttgat gctcggggcg ataaagccgt
    33301 gttccagcat cagcagagag tagatagctt cctgtacgcc agcagcgccc agagagtgac
    33361 cggtcatggc tttggttgca gagatcgccg ggctcttatc gccgaacact tcacggatag
    33421 ctgccagctc tttcacgtcg ccaaccggag tcgaagtgcc gtgggagttc aggtaatcga
    33481 ttggggtatc aacgccatgc atcgccatct gcatacaacg tactgcacct tcgccagacg
    33541 gagcaaccat gtctgcgcca tcagacgttg cgccgtagcc aacgatttca gcatagatgt
    33601 gagcaccacg cgccagcgcg tgttccagct cttcaaccac taccataccg ccgccgccag
    33661 cgataacgaa accgtcacgg tgagcgtcgt aagtacggga ggctttttcc ggggtgtcgt
    33721 tgtatttagt agacagcgca cccattgcgt cgaattcgca agccatttcc cagcacagct
    33781 cttcgccgcc gccagcaaac acgatgtcct gtttgcccag ttggatctgc tctactgcgt
    33841 taccgataca gtgtgcggaa gtcgcacacg cggagctgat ggagtagtta acgccatgaa
    33901 ttttaaacgg ggtggcgagg caggcagaaa cgccggatgc catcgctttg gtgaccacat
    33961 atgggccaac cgctttcagg ccgcgcggac cgcgcattgc gtcagcgccg aacacctgga
    34021 aacgcgggga gccgccgccg gaacctgcaa tcaggccaac gcgcgggtta ttctggtaag
    34081 cttccggaga aaggcccgca tctgcgattg cctgctccat agaaaggaat gcataaatgg
    34141 atgcgtcgct cataaagcgc acaactttgc ggtcaatgag gccagtggta tccagtttta
    34201 cgttgcccca gacgtggcta cgcatgccgg aatccttcag ctcctgagag aaagtgatcc
    34261 ctgaacgtcc ttcacgcaga gatgccagga cttcctgctg gttattaccg atgctggaaa
    34321 caatgcccag gccagtaatc actgcacgtt tcattcaata cctctgtaag tcgcacatag
    34381 agtaagtttc gaatgcacaa tagcgtacac ttgtacgccg aacaagtccg atcagccaat
    34441 taatagagaa atttgcgcag ccttacacac atcgctaaga tcgagccacc gcctgtaaga
    34501 cgagtaactt acgtgaaaca ctactccata caacctgcca acctcgaatt taatgctgag
    34561 ggtacacctg tttcccgaga ttttgacgat gtctattttt ccaacgataa cgggctggaa
    34621 gagacgcgtt atgtttttct cggaggtaac caattagagg cacgctttcc tgagcatcta
    34681 catcctctgt ttgtggtagc agagagcggc ttcggcaccg gattaaactt cctgacgcta
    34741 tggcaggcat ttgatcagtt tcgcgaagcg catccgcaag cgcaattaca acgcttacat
    34801 ttcattagtt ttgagaaatt tcccctcacc cgtgcggatt tatccttagc gcatcaacac
    34861 tggccggaac tggctccgtg ggcagaacaa cttcaggcgc agtggccaat gcccttgccc
    34921 ggttgccatc gtttattact cgatgaaggc cgcgtgacgc tggatttatg gtttggcgat
    34981 attaatgaac tgaccagcca actggacgat tcgctaaatc aaaaagtaga tgcctggttt
    35041 ctggacggct ttgcgccagc gaaaaacccg gatatgtgga cgcaaaatct gtttaacgcc
    35101 atggcaagat tggcgcgtcc gggcggcacg ctggcgacat ttacgtctgc cggttttgtc
    35161 cgccgcggtt tgcaggacgc cggattcacg atgcaaaaac gtaagggctt tgggcgcaaa
    35221 cgggaaatgc tttgtggggt gatggaacag acattaccac tcccctgctc cacgccgtgg
    35281 tttaatcgta cgggcagcag caaacgggaa gtagcgatta tcggcggtgg tattgccagc
    35341 gcgttgttgt cgctggcgtt attacggcgc ggctggcaag taacgcttta ttgcgctgat
    35401 gaagcccccg cgctgggtgc ttccggcaat cgccaggggg cgttgtatcc gctcttaagc
    35461 aaacacgatg aggcgttgaa tcgctttttc tctaatgcgt ttacttttgc tcgtcggttt
    35521 tacgaccaat tacccgttaa atttgatcat gactggtgcg gcgtgacgca gttaggctgg
    35581 gatgagaaaa gccagcataa aatcgcacag atgctgtcaa tggatttacc cgaagaactg
    35641 gctgtagccg ttgaggcaaa tgcggttgaa caaattacgg gcgttgcgac aaattgcagc
    35701 ggcattactt atccgcaagg tggttggcta tgtccagcag aactgacccg taatgtgctg
    35761 gaactggcgc aacagcaggg tttgcagatt tattatcaat atcagttaca ggatttatcc
    35821 cgtaaggatg actgttggtt gttgaatttt gcaggagatc agcaagcaac acacagcgta
    35881 gtggtactgg cgaacgggca tcaaatcagc cgtttcagcc aaacatcatc tctgccagta
    35941 tattcggttg ccggtcaggt cagccatatt ccgacgacac cggaactggc aaaactgaag
    36001 caggtgctgt gctatgacgg ttatctcacg ccacaaaatc cggcgaatca gcaccattgt
    36061 attggtgcca gttatcatcg cggcagcgaa gatacggcgt acagtgatga agatcagcag
    36121 cagaatcgcc agcggttgat tgattgtttc ccgcaggcac agtgggcaaa agaggttgat
    36181 atcagtgaga aagaggcgcg ctgcggtgtg cgttgtgcca cccgcgatca tctgccaatg
    36241 gtaggtaatg ttcccgatta tgaggcaaca ctcgtggaat atgcgtcgct ggcggagcag
    36301 aaagataagg cggtaagcgc gccggtttat gacgatctct ttatgcttgc tgctttaggt
    36361 tcgcgtggtt tgtgttctgc cccactgtgc tccgagattc tggcggcaca gatgagtgaa
    36421 gaaccgattc cgatggatgc cagtacgctg gcggcgttaa atccgaatcg gttatgggtg
    36481 cggaaattgt tgaagggtaa agcagttaag gctggttaac cagctttggc attgtttgtc
    36541 ggatgcggcg tgaacgcctt atccgactta cgtgtgacgt gtaggcctga taagacgcga
    36601 taagcgtcgc atcaggcatg gtgctccaga cgccgcaact tactgctgtg acgcctgctg
    36661 aaacaggttt tcccacatat cggtcaccag cgcctggtca cgcggcgaca attcaccagc
    36721 accaatggct ttttccagac tctggctaac ggtcgtatgc accgcctgag cggagtggtc
    36781 atcaccactt tccagttctg cgatggctaa tgtcaggtgg ccacgcaaat acccactggc
    36841 aaacaactca tcatcactgg catggtcaac cataccgtcg attaatgcca gaatgcgtga
    36901 ttcaaactcc gcgatcatct tctttcctca ttgtaaaacg tggcgctggc ttcagttgag
    36961 cgcttccggc cacgggaact gttccgccgt aagttccggc gtgtgataat aattttgtag
    37021 tgctttaatg aagcgtgccg ggcgttccgg aatgccattt gtcagataat ccatcacctg
    37081 cgcatgtact ctccgttgaa acaccacacg atccggttcg aagtcgcctt ccagattgtc
    37141 gcaactgacg ttaaacggat atcccgccgc cacgcagaac aaccagtcaa gcgcctgcgg
    37201 cttcacttca acatcttcaa actggctttg cgtttgggca tcgcgaccgt ccgggcaata
    37261 ccagtagccg aaatcaacct gctcgcggcg cgctttcccg gcaatacacc agtgcgaaat
    37321 ctcatgaatt gcgctggcat aaaagccgtg ggcaaagacg attcggttat acggtacttc
    37381 cgcatcagcg ggaagataga tcggttcgtc gtcgccttta attagacagg tattaaattc
    37441 atcggcaaag cagctattaa aaatttcaat taactgctca tagtggtgtg tactgttcat
    37501 tagttcatcc ccaaccagtg gaggatctcc tgtccgtggc tatcataaag taatttggca
    37561 ctcatcaccg ccgagacgat aacgatcatc gggcggatca gcttttgtcc tttgctcaac
    37621 accagtcttg aacccatgcg cgcgccgagg aactgcccca ccagcatcac aaagcctgtc
    37681 gcccaaataa ctttgccgcc gagaataaac agcagcaaac cgccgatgtt tgacgttgcg
    37741 ttgagcaatt tggcgtgagc cgtggctttg gcgaggttaa atccgcacag cgtaacgaag
    37801 gccagcgcgt aaaacgaccc ggcagccggg ccaaagaatc catcataaaa accgacgcag
    37861 ccaccggcaa ttaacgcgaa gggtaaaccg tacatccggc gctggcggtc ttcttcaccc
    37921 agctttggca tcagcaagaa atagaggccg atacaaatca ccagaatggg caaaatctgc
    37981 cgtaagacat cagcctgaac gtattgcacc agcagtgcgc cgctcattga gccgataaag
    38041 gtcatggcga tattgagttt ctgatcgctt aaactaacca ctttgcggcg aataaagtag
    38101 atagtggcgg aaatagagcc gccgcaggct tgcagtttat tggttgccag cgcattagcg
    38161 ggagacatcc ccgctgccat taatgccgga atcgtgagta acccaccacc accggcaatc
    38221 gagtcgataa atcccgccag catggcgaca aaaaagagaa ctcccagcaa cagcggggaa
    38281 accataaaca ggctattaaa cgtttccatt agatcacgtg ctcatccagt agcgcctggc
    38341 aggaaggcgg caacggaggc ggtgtcttct tctcaggctt tgttgttccc ggttttggag
    38401 gttcaaacca gctttgcagt tctgccccgc aaccatcgcc tgatggcggt aaaggttgat
    38461 cttcacactc cagactatcg gcagggcaac gtaatcgtac atgcatatgc gcgcgatgct
    38521 ggaaccaggg tcgcactttg cgcaaccagt cgcgatcggt gcccgcatca aggcaaagtt
    38581 gttgtttaat cgccggatta acaaaaatgc gcgtgacgtc tttgtcctgg gcggcgagtt
    38641 tgatcaagct gaaaatttct ggcttccaca gcgtggagac aacgtgttta ccgtcgcggg
    38701 aaactaagtc cagtgcttgc gggcgcaaga gctgcgcgga ggtccagcga gttttcggca
    38761 gttgcagaaa gatatcgaca tccagtccgg tctggtggct ggcatgaccg ccgttgaaac
    38821 gcccaccagc gggcatcccc atatcgccaa tcagcaccgt acccatgccc agattgctca
    38881 cctggctact cagacgctgg ataaacatca ccaggtccgg gtgaccgaaa tagcgacgct
    38941 gatctgtacg catgacctga taatgttcgg actgtatcgg cagcgtgtca gcgccgacga
    39001 tacagccatt agaaaaactg cctatcgatt gtgcgctacc cggcacaggt tgggttattt
    39061 tttgccacgg cgtcgctgcc aggctgacgc tactggcaag cagggccagc agcgcaatcg
    39121 cggttttgtt cattttttac cagcgtggaa tatcagtctt cacatcggca ttttgcgccc
    39181 gctgccgtag caggtgatcc attaaaacga tcgccagcat cgcttcagcg atcggcaccg
    39241 cgcggatccc gacacaagga tcgtgacggc ctttggtgat catctcaact tcttcgccaa
    39301 agcggttaat ggtgcgcccc ggcacggtaa tgctggaggt cggtttcagc gccatatggg
    39361 caatgatttg ctgcccgctg ctgataccgc caagaatgcc gcccgcatgg ttgctctgga
    39421 aaccgtcttt ggtgatttcg tcgcgattct ggctgccgcg cagcgccact acgtcaaaac
    39481 cgtcgccaat ttccacgcct ttcaccgcgt tgatgctcat cagcgcatgg gcgatgtcgg
    39541 catccaggcg gtcaaagacc ggctcgccaa gtccggcggg gacaccactg gctacaacgg
    39601 tgactttcgc gccgatggag tcgccctctt ttttcagcgc gcgcatcagt tcatctaacg
    39661 cgtcgatttt gtccgggtcc gggcagaaga acggattttg ttcaacctgc gaccagtctt
    39721 tgatttccag cggaatgtcg cccatctggg tcaggcagcc gcggatttca ataccaaatt
    39781 tctcggcgag atattttttg gcaatcgccc ctgccgccac gcgcatggcg gtttcgcggg
    39841 cggaagaacg tccaccgccg cgataatcgc gcagaccgta tttttgttcg taggtgtaat
    39901 cagcatggcc tgggcggaaa acgtctttaa ttgcgctgta atcctgagaa cgctggtcgg
    39961 tattttcgat caacaagcca atgctggtgc cggtagtaac gccttcaaaa acaccggaga
    40021 gaatcttgac ctgatccggc tcgcggcgct gggtggtata gcgcgatgtc ccagggcgac
    40081 gacggtcgag gtcatgttgc aggtccgctt ccgtcagcgg aatgcctggc ggaacaccat
    40141 cgacgatgca gccgagcgcc agcccgtgcg attcgccgaa ggtggttacg cgaaagagtt
    40201 gtccaattgt gtttccagcc atcacggctc cgttattgtt gtgtttgcgt gtttacttaa
    40261 tctttataaa tcgcgaaatg ttcacgggcc gccagcagct gctctttggt gagcataaac
    40321 acaccatcgc cgccgttatc aaactccagc caggtgaacg gaacatccgg atattgttcc
    40381 ataagatgta ccatgctgtt accgacttca caaatcagta cgccatcatc agcaagataa
    40441 tctgccgcgt taccgagaat gcgacgcgtc agtttcaggc cgtcggtgcc agatgccagg
    40501 cccagttccg gctcgtgacg gtattcgttt ggcaggtcgg acatatcttc cgcatcgaca
    40561 tacggcgggt tagtgacgat caggtcgtac tgcactttcg gcaaatcacg gaacagatcg
    40621 gaacgaatcg ggatgacgtt gtggatcaga ccgtgttctt cgatgttctg ttcggcaacc
    40681 gccagcgcgt cgggagagat atccaccgcg tcgacttccg catccgggaa ggcataagca
    40741 caggcaatgg cgatgcagcc gctaccggta cacatatcta aaatatgctg cggttgcttg
    40801 ctgattagcc cggcaaattt attgttaatc agttcaccaa tcggcgaacg cggcaccagc
    40861 acgcgttcat cgacgtaaaa ttcatggccg cagaaccagg ctttattggt caggtaagcc
    40921 accggaatgc gttcgttgac gcggcggatc acgcgctcaa caatacggtg tttttcgctg
    40981 gaggtcagac gcgcagtgcg catatcttcc ggaatatcca gcggcaggta gagcgacggc
    41041 aacaccagtt gtaccgcttc atcccacggg ttatcggtgc cgtgaccgta ccagatattt
    41101 gccgcgctga agcggctaac cgaccagcgc aacatgtcct gaatggtttg cagctcatta
    41161 actgcttcat caacgaaaat tttatccacg tattcctcca gggcatgatc gcaataattt
    41221 cggcggctag tttgccacga agatgacgat aaatcagcat tcacgcgcgg tgagtgagga
    41281 aaaatacgtt taaaacgatc gattgtgcta cgagtcgggt aaactgtagg aaaattagaa
    41341 atagagacag ataaatgaaa aagaaaacaa cacttagcga ggaggaccag gctctgtttc
    41401 gccagttgat ggcggggact cgcaagatta agcaggacac gattgtccac cgaccacagc
    41461 gtaaaaaagt cagcgaagtg ccggtgaaac gcttgatcca ggagcaggct gatgccagcc
    41521 attatttctc cgatgagttt cagccgttat taaataccga aggtccggtt aaatatgttc
    41581 gcccggatgt gagtcatttt gaggcgaaaa agttgcgacg cggtgattat tcgccggaat
    41641 tgtttttgga tttacacggt ctgacgcagc tgcaggccaa gcaggaactg ggcgcattga
    41701 ttgccgcctg ccgccgtgaa catgtgtttt gtgcctgtgt gatgcatggg catggtaagc
    41761 atattttgaa acagcaaacg ccgctgtggc tggcgcaaca tccacatgtg atggcatttc
    41821 accaggcgcc gaaagagtac ggtggtgatg cggcgttgct ggtgttgatt gaagtggaag
    41881 agtggctgcc gccggaattg ccgtgaatat taagtgccgg atgtggcact ggcaccgaat
    41941 gccggatgcg gcgtgaacgc cttatccggc ctacaaagtg tgcaaattca atagattgtg
    42001 attcaattgt aggcctgata agacgcgaca gcgtcgcatc aggcatcagc gcacaactat
    42061 agttcattca gtacatactg gaaacggcat gtagaatcaa ttggcgcacg gctactggta
    42121 ttcacctgta ccacaatgtc gtgtagcttt ttgtccgtca ccggttgacg tgagggggtg
    42181 actgtcacct ttgcattatc acaaccatac gaggtaaagg ttaataagtc cgaaacagca
    42241 cgacaatatg cccctctgga atcaaaaaca ctgccatcac agagtttgcc gctttccatc
    42301 aggtaggtcg aaagcgtagt cgtgatagtc gaaccatcgg attgcaaccg caacgcttta
    42361 ttatggctaa cgtaattctc actccatgat atatcaaaag aagcaaccgg aatcgtgggg
    42421 caagcggaat catgtaccgt tggactggta taaacaccaa atcctacctg ttctgaaact
    42481 gaataaaatg acgcctgcca tgggcaacca taattatcag actcgacagt gacatcaaac
    42541 gtcatatatg cacagtaaaa attcttcgta tttatacctg gacaattcga ggtatcagtc
    42601 cgaccatagt cggtcaattt caactgtggt gcaggcccgg aaccggtcca ggaaatcacc
    42661 gggagtatgt cgccacgccg agcgtccgaa ttatattgac cataacttac ctctcctggt
    42721 gtcagcaccg tgacataaaa aaacaaatca cccacaggcg atattctatt catcccataa
    42781 taaatctcag tacttttaaa agttgaactc atgagcttac taccagcata gctctttgat
    42841 gttcccacat ttactataag taacagaagc gccgctaaaa aaaatgtcca ttttttcata
    42901 ttcaaaaata ctcaaaagtt accgtcatcg tggcggtgaa atctccggac gtaacatctc
    42961 gtccatttac ggcctgtaac cacgtattaa aattgacact gttatttccc tgatttaacg
    43021 ggaatgtggc acctgtgttg ctgttaatag gtacagccgc ccctccggca gtttcaatgc
    43081 caatcccaac acccgttgca gaagatgatg aatcaatcga cagaaatcca ggtaagtcgg
    43141 tgtcttctgt ccccgtcaag gtcaccttga cattaaacgc cgtggcactt ttgcaatctt
    43201 tcaactgaaa gactaatggc acacgctctg actgaccacg tagcattaaa tcgctggccg
    43261 caattgtggg gaaatggatt tccgcaagta agttgccatt gattacagga gtacaggatt
    43321 tcccaaacaa attaccggta aaatgcagat tatcggcagc ggagactgtc ccactaaata
    43381 acagcagact acaaataagc accaacacac tgtttttctt cattggtaat ccacctgtag
    43441 cgtagcccac gcctcaaagt ccgcctctgg taaatcgaca ccactctttt ttacgggaac
    43501 ggctgttatc acaggaaggg ctgtttcatt caccactaag ggggtattca gcttaaaatc
    43561 gcttccggct tgtttaagat gtattgctaa tccattgata tcggtggtta cagccgcatc
    43621 atcaaagtca ctctgtgtgc cactccaggt caatgtcatt tccatttgag ggtcacgaac
    43681 agtggtatca caggtaatgg catatggcac aaactgctca taattgctgc cgttaatgtc
    43741 atcaatattc acgtcccgaa acgtcaactc tatattttct ccattactaa ttgtgcaatt
    43801 cggtggctgc actaaataac catgaaaaga aatattcgag agcgccatag agctaatgcc
    43861 ccacaacatt aagagagaaa tccgtttcat tgataatcca ccacaatagt ggcactggcg
    43921 ctaaattccg ccgccgttag agatacaccg ctctgcacga caggaaccgc ttccagtgcc
    43981 ggtgcgcccg atgaaaagtt aaacggtaac catgtgccag gcgttaaatc cagaatggag
    44041 tgatcgctgg ctttttgaat ccgaattcca aaaccggcaa tacccgtatc gattaccgtt
    44101 tctccgttaa tcacggttgt agttgcctgc atatttaagc gcaggtcgtc cattgccaga
    44161 ttgttgcaca caagcgagta gtcaatgggg cgtttgtaac ttaccccatc gatcttagaa
    44221 ataaatacat taccgaactc cacgctcgca ccagttaccg tacagggcgg cggggcatca
    44281 acgagcaccc gcaagaccat cgcaaccgat ttgctacccg ccattacagg cgtgctcaca
    44341 atactacata gcaataacca cacccaagcc gaataagatc ctttcatatt agtgctccta
    44401 tccctggccc tggctgtttt gcttttcatc cacctggcag gtgtttcccg tacaggtgaa
    44461 gaacagcggc atccgcgcgc catagtcgtt gacgtaggtc agcaccggga cgctatccat
    44521 tttgaccttt aacggtacgg aggtttttgg cgatatcacc agcggggaaa atcccgctgc
    44581 cggattgccg tttttctgcg cgctggcgtt actgataacc acgaagtacg gcgtcgggtt
    44641 attgactgta aactcctggc cgctgcgggt taaggtcacc ttatgctgcc acggattttt
    44701 catgctgacg ttttccagcg cttttggtcg ccagaacagc ttgatgcgcg tttgcagcgc
    44761 aatctgtaac gtgttgggtt tgttcgattt cggcgggatc tcacgcacgt tgaaatagaa
    44821 cacgctctcg cggtcggcag gcagcttgtt gatatccggc atgccctgta ccttcacctg
    44881 accgttcatc atggaatcga tgcgctgcac cggcggcagt accgtcagtg gcgagctaat
    44941 cttgttaccc tgttcatctt ccatccagct ttgtgccaga tacggcattt tcgggtcgtt
    45001 attgcgcagc gtgacgctga ttgatttatc actttcgtta aaaatcaggc gtgtacggtc
    45061 cggcgtgaca gaggcctgtg ctgacaggct ggcggcagtt atcagtaacg ccagcgtcgt
    45121 tgcggccgtt ctggcagagc aaaatagatt cgtcatggat cattccttaa tcacgttgtt
    45181 aattggcgga taccgatacc ggcgcttccg ttggcatcac ctgctgggtc tgctcctgga
    45241 tcaccggctt aatttgatga ggcatcgaag gagatatcgc ccctgtttgt tgacatggta
    45301 gtaacaggcc attgaacaga tcggctggca gtgggtccgg cagatgaata tcacagtggg
    45361 ccacaccgcc ccatgaaacg atcatatgct cgccaggttt gacacccgcg aggtagacgt
    45421 tgccgtcatc atcaaccaga ccgacgttct gcgcgttgtc atttttcact tccgcgccaa
    45481 acggcggatg actgccatct tgcaggcgca ataccgccat cgctttttgc ccgctgataa
    45541 ccgaaaactt acggtagcca atggctcctt cggtaagcgt cgcctgcacc acggactggg
    45601 tggcttcggc gttttccggc aggttgttaa ggtcgatata ggcctgattg cggtagtagt
    45661 tgttgacgtc tgccaccacg gtcttaccga acatattggt gtaaaccgcc gcgccatttc
    45721 cttccaccgg aacaccagcc acgccatcgg catcaatcag caggcgcgta ccgcccatat
    45781 tctgggtacg gtggagcgcg ccaccctgcg cggtcagcgt cgcgccgccc tgtaaggaaa
    45841 tacctgccga ggtgtactgt ccttcatggt agttggcgct gaggtccacc tgcgccagcg
    45901 agccatcgtg gctgtaatag ccgtcaacgc tggagtgttt gtcactggtc cccacgttca
    45961 gctggtagtg agttgcgtca tcgacacggc tgaaataccc cacctggctg ctgtccgaac
    46021 cgctgccgta gttgccgtta tagctgatgg tgctgctgtc accccacggc atactgagcg
    46081 atatgtacac acctttatcg gcctggttat catactcgta gcggtatccg gtcatggaaa
    46141 tgctcatatt gcggatactg cccaggttga agtagtgcga gagcatgacg ttgtagttgg
    46201 tctgttcgtc gcgatcccag taggtatggc gggtgtagtt gagataaaca gacacaccgg
    46261 catccctgaa gttctggtta taggtggcgg tgtacatctc tttgtcgttg ccggtgcgca
    46321 ccatttcgct gtcgctggca tcgagatact cgctcatggt catgaagttc tcttccgaga
    46381 agcggtatcc ggcaaaagtg acgcggctgt tcagttcatc gaagtctttg gaatagctca
    46441 cgcgaaacga gttaccgtcc agtgaacctt taccgtaggc ggtctcttta tccaggcggg
    46501 tatgcgagtg ggtgatatca aaggcgactg cgccaaagac agacaggtcg cgaccgacgc
    46561 caagcgccgc cgactgatag tgttcatctg ccagcgcccc gccgtacagc gaccagccgt
    46621 tggcaatccc ccatgaagct tcgccgccgg agaagaaacc gccctcaaca tgatgccccc
    46681 actcctgcgg gcggcccatc atcagcttat agcgcacctg gccggggcgg gtcaggaacg
    46741 gcatcgaggc ggtgttgatg tcatattcct gcacctggcc gttctgttct tcaatgcgga
    46801 tgtgcagtgt accggagaca gaatcgccaa gatcctggat gcggaacggc cccgccggaa
    46861 cctgcgtttc gtaaatcacg cggcccatct ggctgacggt cactttcgcg gtggtgtgcg
    46921 ccacgccgga gatatccggc gcatagccgc gcagattcgg cggcaacatt tgatcgtcgg
    46981 tgctgatact gccacccacg tagttaaagc cgtcgaaaat atcagaatta aggtagtctt
    47041 cgccaaggcc aagtttggct tttagcgacg gtaaggcccg ccaggcgtag tagcggctcc
    47101 actcccagtt tttttgcgtg tcatcaccgt tgataacatc gtcatcattg cttttgctgt
    47161 gctggtagtc agtctgccag tcggctcgca gacgccatgc gccgaggtta accccaaccg
    47221 ttccgttgcc gctgatttca ttgctgtcat ccccgccatt ttcttcatgt cgtgtctggg
    47281 cggtaatact gtagtcagca attaaaccag atataccgtc atcccagcgt gaaggcggat
    47341 cccagttgat gtcggtatat tcaaggtaag cctggggtaa tgaaatgact aacgctgact
    47401 gactcaggtc agctttaata tcaatgcctt ccagttgacc gggtttcagg catttgccgt
    47461 cgtggatcca ttgcaggttt tttgccacat cctcttttaa gccaaactgc gcgaccagtt
    47521 caggcgtcag gcaggcatag gttttactgg catcgttctc agaggcgtac cagtaaatat
    47581 cgtattcttc cgccagcggc tgtttattta gttgaacctg taaattgtat ttaccaggtt
    47641 cgacatatcc ctggctggaa aatcgcttca gatcgatttt tgtgtcgcct tttaactcca
    47701 gaaaacggga atcaaactga atttcatctt cagcccaggc attgacataa caccctgaca
    47761 ttgccagcgc aatgcagcag ggaagcaccc gcagtcggaa aagagaatgg tcaggcatac
    47821 acgtatccat ccgtaattat tataatttgc gcgaattcca tttaaaaacc ccatagcccg
    47881 aaagctatgg gggtatcact tagagataag taatctggaa ggtcgtgttg gcttcaaaat
    47941 tacctaaatc tggcgcatca gcagcgccca ccagccaggc tttaaagtcc agcgtctgct
    48001 tcgctttgcc tttgttggtc gccgtatctt ttacgatttt ctggtcgata cccgcgccgc
    48061 ttttgtaaga ggtcccctga gcgtcaccaa tggccaggct gacattgtta aatgccgcac
    48121 cggtatcggt attgtaaatg gtgtagtaat tgccgctgtt ggtagaagac gcgttacccg
    48181 tgaaggtagt ggtcatcgtt gtctgggtgt caaacacaca atcttgcaga tggatctgga
    48241 aagctttcgg tgtagaagtc ccgttgtttt tcaggatccc tgaaccaatg tcacccatat
    48301 ccacttccag tttgtgatca tcaggaacga tggagcacgg ggaatcttca atggtgccgt
    48361 aaaaacgagc tgtaccgttg ttgccagcgg cgattgttga agtagaagcg aacgcaccca
    48421 ttaccattgt tgcagcaata gctgttttta caaacttact cattatttgt ccttaaacca
    48481 taaataat
//
LOCUS       DAPGOF010000011        48000 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.011, whole genome shotgun sequence.
ACCESSION   DAPGOF010000011 DAPGOF010000000
VERSION     DAPGOF010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48000
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.011"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..162
                     /locus_tag="RHV13_000544"
     CDS             <1..162
                     /locus_tag="RHV13_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HDV1240104.1"
                     /translation="KFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISISKI
                     IGGCGPTAQ"
     gene            complement(269..1354)
                     /locus_tag="RHV13_000545"
     CDS             complement(269..1354)
                     /locus_tag="RHV13_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDV1240105.1"
                     /translation="MKTFPLQSLTLNEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAALAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RLAGIPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGAHALEVLRGLMFAPVMHAVQAGVSERLLALLNSGAVAEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKCGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRDSIASI"
     gene            complement(1366..2670)
                     /locus_tag="RHV13_000546"
     CDS             complement(1366..2670)
                     /locus_tag="RHV13_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HDV1240106.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLI
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWFGLFIPA"
     gene            complement(2682..3035)
                     /locus_tag="RHV13_000547"
     CDS             complement(2682..3035)
                     /locus_tag="RHV13_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HDV1240107.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(3046..3924)
                     /locus_tag="RHV13_000548"
     CDS             complement(3046..3924)
                     /locus_tag="RHV13_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HDV1240108.1"
                     /translation="MSFDPTGYTFAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDIMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(3921..5147)
                     /locus_tag="RHV13_000549"
     CDS             complement(3921..5147)
                     /locus_tag="RHV13_000549"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDV1240109.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIYRVEQALVSAGWQVERRGDELQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVNNPYGVSWQNLVDSQQIMD
                     ITLDEFITHQTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLALLVEAMQPDDCLI
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLAATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(5148..6311)
                     /locus_tag="RHV13_000550"
     CDS             complement(5148..6311)
                     /locus_tag="RHV13_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HDV1240110.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRIQSVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLIQAR
                     DQLAKSGIAIEQLNAPSATSCTSLPLLAQYGVTHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     SLAGEFPVSSAVVLCFRTQIFVTRSDVVLVSGIQLGEPEIVGRYDSLGNPLEA"
     gene            complement(6395..6757)
                     /locus_tag="RHV13_000551"
     CDS             complement(6395..6757)
                     /locus_tag="RHV13_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDV1240111.1"
                     /translation="METRLNLLCDAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSTHWPAILQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(6774..7679)
                     /locus_tag="RHV13_000552"
     CDS             complement(6774..7679)
                     /locus_tag="RHV13_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1240112.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALD
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(7948..8952)
                     /gene="trpS"
                     /locus_tag="RHV13_000553"
     CDS             complement(7948..8952)
                     /gene="trpS"
                     /locus_tag="RHV13_000553"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDV1240113.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGDIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(8945..9703)
                     /gene="gph"
                     /locus_tag="RHV13_000554"
     CDS             complement(8945..9703)
                     /gene="gph"
                     /locus_tag="RHV13_000554"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HDV1240114.1"
                     /translation="MSKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALAWARQERATLRKTMGKPPVDDDIPVEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(9696..10373)
                     /gene="rpe"
                     /locus_tag="RHV13_000555"
     CDS             complement(9696..10373)
                     /gene="rpe"
                     /locus_tag="RHV13_000555"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDV1240115.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(10391..11227)
                     /gene="dam"
                     /locus_tag="RHV13_000556"
     CDS             complement(10391..11227)
                     /gene="dam"
                     /locus_tag="RHV13_000556"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HDV1240116.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVD
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(11334..12620)
                     /gene="damX"
                     /locus_tag="RHV13_000557"
     CDS             complement(11334..12620)
                     /gene="damX"
                     /locus_tag="RHV13_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HDV1240117.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASQAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(12712..13800)
                     /gene="aroB"
                     /locus_tag="RHV13_000558"
     CDS             complement(12712..13800)
                     /gene="aroB"
                     /locus_tag="RHV13_000558"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDV1240118.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREISAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(13857..14378)
                     /gene="aroK"
                     /locus_tag="RHV13_000559"
     CDS             complement(13857..14378)
                     /gene="aroK"
                     /locus_tag="RHV13_000559"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HDV1240119.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(14779..16017)
                     /gene="hofQ"
                     /locus_tag="RHV13_000560"
     CDS             complement(14779..16017)
                     /gene="hofQ"
                     /locus_tag="RHV13_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HDV1240120.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSVAWQNDNIA
                     RQEAEQARAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(15929..16333)
                     /gene="hofP"
                     /locus_tag="RHV13_000561"
     CDS             complement(15929..16333)
                     /gene="hofP"
                     /locus_tag="RHV13_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HDV1240121.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(16323..16763)
                     /gene="hofO"
                     /locus_tag="RHV13_000562"
     CDS             complement(16323..16763)
                     /gene="hofO"
                     /locus_tag="RHV13_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HDV1240122.1"
                     /translation="MNMFFDWWFATSPRLRQLCWAVWLLMLVTLIFLSSTHHEESDAL
                     IRLRASHHQQWAALYRLVDTTPFREEKTLPFSPLDFQLPGAQLVFWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSCFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(16747..17286)
                     /gene="hofN"
                     /locus_tag="RHV13_000563"
     CDS             complement(16747..17286)
                     /gene="hofN"
                     /locus_tag="RHV13_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HDV1240123.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     SAEARIDAVLLQAEQQLARSLQITKPRLLERQQIREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(17286..18065)
                     /gene="hofM"
                     /locus_tag="RHV13_000564"
     CDS             complement(17286..18065)
                     /gene="hofM"
                     /locus_tag="RHV13_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HDV1240124.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQRLAKTLLPWSRELPQRPHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKTY"
     gene            18185..20737
                     /gene="mrcA"
                     /locus_tag="RHV13_000565"
     CDS             18185..20737
                     /gene="mrcA"
                     /locus_tag="RHV13_000565"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HDV1240125.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PLYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(20898..21458)
                     /gene="nudE"
                     /locus_tag="RHV13_000566"
     CDS             complement(20898..21458)
                     /gene="nudE"
                     /locus_tag="RHV13_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HDV1240126.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            21778..23913
                     /gene="igaA"
                     /locus_tag="RHV13_000567"
     CDS             21778..23913
                     /gene="igaA"
                     /locus_tag="RHV13_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HDV1240127.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            23978..24646
                     /gene="yrfG"
                     /locus_tag="RHV13_000568"
     CDS             23978..24646
                     /gene="yrfG"
                     /locus_tag="RHV13_000568"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HDV1240128.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            24657..25058
                     /gene="hslR"
                     /locus_tag="RHV13_000569"
     CDS             24657..25058
                     /gene="hslR"
                     /locus_tag="RHV13_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HDV1240129.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            25083..25961
                     /gene="hslO"
                     /locus_tag="RHV13_000570"
     CDS             25083..25961
                     /gene="hslO"
                     /locus_tag="RHV13_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDV1240130.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(26024..27748)
                     /locus_tag="RHV13_000571"
     CDS             complement(26024..27748)
                     /locus_tag="RHV13_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HDV1240131.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLSGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQTDGMNPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNILILLVIFLANGLTWLVLL
                     LWGELFKLVGITFFKTLFFATDWFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWMGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTADRLQGALAVLVLLVWSLGY
                     FVSIVWRKGQNPLALQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGSYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQQQISEKV
                     LAENVLIAPGSGKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGRAERILFAF
                     DDERYIVYGFDPDKKEWQELTMSLLPRDITKEKLLTAAKDGKLGTKPKAWRDLVVDGE
                     RLDVNLNE"
     gene            28127..29749
                     /gene="pckA"
                     /locus_tag="RHV13_000572"
     CDS             28127..29749
                     /gene="pckA"
                     /locus_tag="RHV13_000572"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDV1240132.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTKQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIENIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(29825..31177)
                     /gene="envZ"
                     /locus_tag="RHV13_000573"
     CDS             complement(29825..31177)
                     /gene="envZ"
                     /locus_tag="RHV13_000573"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HDV1240133.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(31174..31893)
                     /gene="ompR"
                     /locus_tag="RHV13_000574"
     CDS             complement(31174..31893)
                     /gene="ompR"
                     /locus_tag="RHV13_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HDV1240134.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            32121..32597
                     /gene="greB"
                     /locus_tag="RHV13_000575"
     CDS             32121..32597
                     /gene="greB"
                     /locus_tag="RHV13_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HDV1240135.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            32694..35015
                     /locus_tag="RHV13_000576"
     CDS             32694..35015
                     /locus_tag="RHV13_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDV1240136.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLGYLRELEERRQAILKSISEQGKLTDDLANAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNDRPQ
                     NNRPAAKPRGREAQPAGNSAMMEALAAAMGKKR"
     gene            35453..35680
                     /gene="feoA"
                     /locus_tag="RHV13_000577"
     CDS             35453..35680
                     /gene="feoA"
                     /locus_tag="RHV13_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HDV1240137.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            35697..38018
                     /gene="feoB"
                     /locus_tag="RHV13_000578"
     CDS             35697..38018
                     /gene="feoB"
                     /locus_tag="RHV13_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HDV1240138.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            38018..38254
                     /gene="feoC"
                     /locus_tag="RHV13_000579"
     CDS             38018..38254
                     /gene="feoC"
                     /locus_tag="RHV13_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HDV1240139.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            38457..39335
                     /gene="rpnA"
                     /locus_tag="RHV13_000580"
     CDS             38457..39335
                     /gene="rpnA"
                     /locus_tag="RHV13_000580"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HDV1240140.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDIT
                     VMPDDEIMQHRRMALLELIQKHIRQHDLLGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALYIAKIMLESGVPLADI
                     MRFTGLSEEELAAASQ"
     gene            complement(39362..40132)
                     /gene="bioH"
                     /locus_tag="RHV13_000581"
     CDS             complement(39362..40132)
                     /gene="bioH"
                     /locus_tag="RHV13_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HDV1240141.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGAMSLADMAEAVLRQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFRHVLVALKQRV"
     gene            40170..40853
                     /gene="gntX"
                     /locus_tag="RHV13_000582"
     CDS             40170..40853
                     /gene="gntX"
                     /locus_tag="RHV13_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HDV1240142.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAITRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            40912..41487
                     /gene="nfuA"
                     /locus_tag="RHV13_000583"
     CDS             40912..41487
                     /gene="nfuA"
                     /locus_tag="RHV13_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HDV1240143.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            41848..43164
                     /gene="gntT"
                     /locus_tag="RHV13_000584"
     CDS             41848..43164
                     /gene="gntT"
                     /locus_tag="RHV13_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HDV1240144.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(43209..45293)
                     /gene="malQ"
                     /locus_tag="RHV13_000585"
     CDS             complement(43209..45293)
                     /gene="malQ"
                     /locus_tag="RHV13_000585"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HDV1240145.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLETMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(45303..47696)
                     /gene="malP"
                     /locus_tag="RHV13_000586"
     CDS             complement(45303..47696)
                     /gene="malP"
                     /locus_tag="RHV13_000586"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HDV1240146.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
BASE COUNT        11436 a        13218 c        12087 g        11259 t
ORIGIN      
        1 aaatttatta aagttgcact ggtaggtgca gtactggcta cgttaactgc atgtactggt
       61 catattgaaa accgtgataa gaactgctct tacgactacc tgctgcaccc ggcaatttct
      121 atttctaaaa tcattggcgg ttgcggtcct actgcacagt aagtctctga cagaaatcgg
      181 ctaacaccgg tttaattgcc cgacgccctc tggattttcc gagggcgtat ttgtttctgc
      241 attcttttcg aaaacatcat tcacaagatt aaatactggc aatactatca cgcaaaatcc
      301 gcagcaccgt ctcttcaccg ctacgattcg ggttgatgcg aatcgcgcaa tgttctaatt
      361 gcggattcgc ctgacgaaac gtcccggaaa gtcgataaaa gagcggcgga atttcatatt
      421 tcgactccgc gcccaccgga taaggcaacg cgccgcattt ctgcgcttct tccagcaccc
      481 tggcggcaat cggctgatga aactccacaa tcaacacttt cgactgcgca ttggcaatga
      541 cggcgctttt cacttccgcc accgcgccgc tgttaagcaa agccagcaac cgttcggata
      601 ctcccgcctg caccgcatgc atcactggcg cgaacatcag cccacgcaac acttccaacg
      661 catgagcgcc ctggatctgg ctgccgccgg agtaaagcgt ggcgcgaatc cgactaataa
      721 catcggcatc gccaaccact gcaccaacgc cctccggccc aaacagcttg aagcaggaga
      781 atgtcgagac attcgcgccg cattcacagc cgattcgcgc caccttcatc accgcatagt
      841 tgtcatcggt taacgctgga atgccagcca ggcgcaacgt tgccagcaca tctgccagca
      901 catagctgtc ctgcggctgc tggcgcgtat gctgcaccag cgcggcatca ggttgttgct
      961 cgtcgacgac ctgcttcagt gccgacaggt cattgaaatc agcagtaata agcgtcagcc
     1021 ccatctgctc aataataacc cgtgtcgtcg ggtaaacagg cgcgtcatgc accagaagac
     1081 gctgccccgg tttgagcaaa gccgccaacg ccgcacgaat cgctccagta cccgcaccct
     1141 gtaccagcgc cgcggcctgt gcgtgaaatg catcagccag cacctgctcc acccgttggg
     1201 taatacgcgg ttgattcaga cctggagtta accctaaatc accgccagta agaaactcgc
     1261 tgccggggaa atggcgacaa atgctatcca ccagcgcaaa ctgcttttgc tgcgcttcat
     1321 taagcgtcag actttgcaga ggaaacgtct tcataggtgc cttccttaag ccggaataaa
     1381 caggccaaac cagtaaagaa cattcagcag gatgccggtg atcattactg ccacgaccgg
     1441 tgccgccatt ttctgtaccg gacggcccag ggattcgttg aggaagtaaa tggcaaccgc
     1501 gatagagaat ccggtataac ccgccatctt aattgctgcg aaaatcgaac cgaccagcag
     1561 cgccacttcc atcagcatat tcatggcgtt acggatgtta tccgacgcat tacgcaccga
     1621 cgggtagcgt cccagccatt tgccgataga acgaagcagc aagacttccg ccgaaataac
     1681 cactgcgcct aataccgctg caaccatcgg attcggcgag agatagccca ccgcatacac
     1741 aaaggtaaag cccgcaactg catacacacc cgttgctaag gcggtggtgg caatcaacgg
     1801 tacaaaaccc agcccgcgca taaattctgc cagtgccgcc tggttgatca gcgtttgcga
     1861 ttgttccggc gttacgcctg cggagtacgc tttctccaga gtgaagatcg acacttcact
     1921 gccggcgaaa atcttcatac tggcaacggc ggcaatcaat gctcccacga tggcgatata
     1981 gggtaagttt ttgataatcc gcgacgtgcg ctcttcaaac accgaaagcc cgctggcatc
     2041 aatgtcattt tcatcacgat gacgcaggtc gtgagttatc gcgatcccca gaagcatcac
     2101 catgccaata aagatttcga tggattcagg gttaagatgt gggaaatagc gcacgacgac
     2161 cacacgggtc atcagtacca ccacggcggc gatcagactt tgcttccagc caaactggta
     2221 gaaaatcgcc accaacggga agagtgcaaa agctgaaacc accggcgagc ttaattcccc
     2281 gaggctacct aatacatcca ccggtagcgc ggtcagcagc tggtttactg gcaacagaca
     2341 agtaaggatc aacacgcccc agatagcgcc aagaccaaac gccatcaggc tgtttatcgc
     2401 cagtacgccg aggatatcgg taggaagaaa gagtaaccat gcgtttagca gcccggtttt
     2461 cagggtaaaa gagatcccca ccgaggccac aaaaccaatg ctcagaccaa aagcgatact
     2521 ccccgcctcg cgacggttca tatagccttc aatcagttgc ggcaggatcg ggcggatgcc
     2581 gtcatgaaaa acagccgccg agcgatgcgc cagaagcgat gtcatacccg tcaggcacgc
     2641 caccacgata atctgaatat acagatccat agtcgtgcct tttatttcag atggttaatc
     2701 agcatcggaa tcgcgtgttc gacgtgctca acggaaaggc caaacgccac ttttccttca
     2761 gcgatcattt tggcgatatg ctcgtctttc gctttaatgc ctggtttggc aatggtgcaa
     2821 cttttgttat agccaatcac cgcgatggca atcgacaatg ccgcgcccgc accggtatta
     2881 cacgcgccga tgtaataatc cagttgcccg gatttcacct tcattgccgc ttccatgtcg
     2941 ttgtgaatga aaacttcaaa acagccagga gccgtcgctt caatagtttt tttaatctgc
     3001 tcacgctgta agcctgcaac gccaatcttt ttcatgagtc tgtccttatt ggaaaaattg
     3061 agagggattt tcacgtaaca tcacatccac atcggcctga ctgaatcctg actggcgcaa
     3121 ttgcggaata aaggtggtta gtaaatagtc atagccataa ccaccgttgg cttttaaatg
     3181 ggagcggcgc gtaatatcca tcgacagcat gacgcggttc agcaacccac ggtcgcgtag
     3241 cgcatgaagc atcgcaatac gcttttcgtc cgggtagtaa ctgttcttac caatggtgtc
     3301 gaactgcacg tacgcgccga gatcgatcat cttcaaaatg ttgtcgaggt tgtctttcag
     3361 atcgcagtga ccaacggtga cgcgcgaaag atcaactccg tgggcttgca acaaagccag
     3421 ttgctccagc cccatcgtgc tgaacgacgt atgcgtggag atcgggcgtc cggtctggtt
     3481 atgtgccagc gcggcggcaa taaatacctt ctcttccagc ggcgtaatct tcccttcgct
     3541 ggagccgatc tccgcgatga tcccggcttt cagttccgtg ccatcgatac cctgctcaat
     3601 ttcatcgacc atctcctgcg ccagttcctg cacgctgcgg gtcgccacat gttccgggaa
     3661 aaacgcgtcc tggtaataac cggtacaggc caccacgttg atccccgtct cgcgcattat
     3721 atcgagcata aattgcgcat tgcgccccat gtaacggttg gtcatctcaa tcacattacg
     3781 cacgccccgg gtcatcaggt cgttcatctc ctggcaaatg aacgcatact gatcaaggcg
     3841 gcagtccacg ttgtttttaa agccggaaag atcaatatgc agatgttcat gggcgaaggt
     3901 gtagcccgtc ggatcaaaac tcatagggtg tctcctgcaa accggaggga ggaaagaaaa
     3961 gagcgaccat tttgtggcgg tggcgcacgg aaaaattcac acacggtagc ccccacatca
     4021 gaaagcgtgg tgcgaacacc tagctgagta gcagccaggc cttgctgata aaccagcacc
     4081 ggcacgactt cgcgggtatg gtggctgtga ccgattgtcg ggtcgttacc atgatcagcc
     4141 atgacgatca ggcagtcatc tggctgcatc gcctcaacca ggagagcaag attacggtca
     4201 acgacctgca aacgttcggc ataacgtgcg acgtcttctg cgtgaccagc gaggtcggtt
     4261 tcctgaatgt tggtgcaaat aaacgctgtc tgatgggtga taaattcgtc gagggtgata
     4321 tccataatct gctggctatc caccagattt tgccagctca cgccataagg attgttgacg
     4381 atatctgcca ccttacccac cagcacggtt ggcacgcctg cttcatacag tttttgtggc
     4441 acctgcactt tttcatcgac gccatagccc atatgcacga cctggaaacc gttgtcataa
     4501 gcgccagaac gcggcgcatt aataccaata aagcgccctt ctttgctttc tgcggcatcg
     4561 agaatacgct gactgtcggt taacaggccg ccaaatgtaa tgacccgacc gacctgtacc
     4621 tggtcacgca cgatacgacc aattttgatt gcgtcgtcaa aagagatcac agagagattg
     4681 gcggtaatgt tatagacctg gcctaaatcc gcctcgagat tatcgccaat cgcaaccgcc
     4741 tgattgaccc acagaaattg cagttcatcg ccacggcgct ccacctgcca accggcggat
     4801 actaatgcct gctcaacacg gtaaatcaca tcgcgaaagg gcatccgcag cggcggtaac
     4861 gggcgcgtgc ctaaaatttc ctgatgcccc ataaaggtat cgccaccttc atgttgcagc
     4921 tctgccacgc cccaggttgc ggagtctgac ggctgcatat cgcctggcgc ataacccaat
     4981 gcgttgatta gccccagctt ctccagcgtt ggtagctgca aatgcggcaa ctggctcagg
     5041 atgtgaccac aggtattcgc cccggcatcc tgcggacgca ccagcgtgac atctttcatt
     5101 gcgcctacgc caaagctatc aatcactaac accacaaatc gcgccattta cgcctccaga
     5161 gggttgccga ggctgtcata acgaccaact atttctggtt cgccgagctg aataccagac
     5221 accagcacca catcgctacg ggtgacaaaa atctgcgtgc ggaaacagag caccactgca
     5281 ctgcttaccg gaaactcgcc tgccagcgac agggtgtagt cgatactgct gtcatccaca
     5341 gttttgagat tggtttcagt aatcttttga ttttctggcg taaacaccag cgcatgttgc
     5401 gcatgaccac gacggtagta accgccgccg tagcagtagc tgtcgccacg gaaatgatgg
     5461 gagatttcac ttaaccagag catcgcaata cgttcaggct gatcgccctg ctggtttgcc
     5521 ggaatagtgc ccgtcagcgc atgaccgggt tcggcatgag tcacaccgta ttgcgccagc
     5581 aatggcaacg aagtgcagct ggtcgcagaa ggtgcgttca gttgctcaat cgcaatacca
     5641 gatttcgcca gttgatcccg agcctgtatc agcgtgtgaa gattcggtgt cggcaaaact
     5701 ttcccggcag cctcatccca aagcaggcaa gggaaatggg ttagtccggc taaatgcagc
     5761 cccggcagtt tcttaatttc agcaaccacc tcgtgcagcg aatgctgtac aaaaccgctc
     5821 tcctgccccg gataaagaaa atcatcgtcg ctataaactt tgagcagcac agactggatt
     5881 cgcccggcct tcaccgccgc cgcagaaact tcccgcgctt tgtcgagagt aaacacggtg
     5941 atgacgtcgg tgccctgttc aacggcgtca gcaacctgat gacaagggat ttgtaccaga
     6001 tgcccctgat gcgccaccgg caaaccagcg cggcgcatga ctcgcgcctc tttgtaatcc
     6061 accgccacaa tgccgctgta gcccaacgcc agcagttttt ccgccagcca tgggttacga
     6121 ccgaattgtt tggtcatcag atacagttca atgccgtaaa gccgcgccgt ctcaatcagc
     6181 cgcttaccgt tttccagtat ctgatccacg tcgatcaccc agctgtccgg ggcgatcttg
     6241 ccctgctgcc acaggcttag tgcggcggaa atcagcgccg ggttctggcg tttcaatgct
     6301 tctacaaaca tcttgttgcc tcgtttctgt tccttgaatt aaatattcat ttttttgaat
     6361 atttaggttg tgcgacgtgg atttgtcggg agcttcaaac ctcttcgtta gcagccatcc
     6421 ataatccata aaggttggcc agtaaatagc cttcttcaca cgggttaact tccagcgcga
     6481 attccttcaa caacacctga tgcaattgta aaatggctgg ccagtgagtg gattgcgcca
     6541 gctctgccag caactcgtta tccagtggct caatttcttc accacggcga ctgcgcatca
     6601 gtgcactcgc catatgtgtc atcgccatcg ttccttgctc actgcgcacc ggcagatggc
     6661 actctgtttc cagtacgttg acgacctgca tcatgccttt gcagatgtcc ttatcaataa
     6721 cgcctgcatc gcaaagcagg ttgagtctgg tttccatatc agatgccttt cgtttaataa
     6781 ctgggttcct gttccccact caccacgcgt tgttgatggg caagcagggc atgtttatca
     6841 acaactgcac gcagcagttg ttgcattggg taatcatcga ctcgcgtcag gaccacggct
     6901 tcggtggcct gtaaaaatcg cggatcgtct gtgagcggcg tcgcttctaa tcccagcatg
     6961 gtcagttcgt tttccgccac cacgttccag ataaccgcat caacatcgcc tttaacaatg
     7021 cgttgtaaac tctcgtgata ggagagatcg actcgttcca catcactatc gccaaaaaag
     7081 acatcggtca tgattttctg atccgccgaa cggttatcca gccccacgcg cttcacgttt
     7141 gcggactcgc ctttacggca aatcaactgg tgctcgccaa cgtaggtgtg cggccccaaa
     7201 tccagcgcga ggcataaccc tttttgcgtg agataacttt ccgccgccag tcgggaaacc
     7261 accgccatgt catacacgcc gttaagtaaa cactccacgc gaatatccgc gccgcgcata
     7321 tgcgcatagt aaaaaggaat gccatcaaac tgggctttca atccgctcgc caggccttcg
     7381 tacaaacggg tatagggcaa gggcatcgcg cacaccacgt tattgatatc cacatgactc
     7441 agcagcgctt tgttatccat ttcgaccaga taactgccat tgcgcccacg gcgttcaatc
     7501 cgtatcgctc cgcttgattc cagcgttttc aacgcggcct gcgtcaggcc aacggatgaa
     7561 cggcattcag ttgccagctc atctatggtt ttcagtcgat taccgcactt ttcacctaac
     7621 aaataacggg ccagcgttgt gataacgacg ccttcttttt tgataaacgt tcgacgcata
     7681 gtaaattttc aataaagtga atatttatat cttcaggaat ttgaagataa ggcgcaacag
     7741 cgagatgtgg aaacggcgag gcgcttcaca ttttttcgga ttattgcaaa tgaggataaa
     7801 aaaaccgggt ttccccggtt tcagagtgat gacgaagtga tttattcgac aatgcgagca
     7861 aagggtaatt cccactggcg cttgcctgct cccctcaccc taaccctctc cccaaagggg
     7921 cgaggggacc gtccgagcgt tttgatatta cggcttcgcc acaaaaccaa tcgcttcgta
     7981 caccgctttt agcgtacggg aagcgtgcgc gctggctttt tctgcgccgt ctttcatcac
     8041 ctgttgcagg aaggcttcat cgttgcggaa acggtggtaa cgttcctgta attcagtcag
     8101 cataccggaa acggcatcag ccacttcgcc tttcagatga ccatacatct tgccttcgaa
     8161 ctgtttttcc agttccggga tgctctggcc cgttaccgca gaaaggatat ccagcaggtt
     8221 ggaaacgccc gctttgttct gcacatcgta gcgaactacc ggcggctcgt cggagtcggt
     8281 gaccgcgcgt ttgattttct tcactacaga tttcggatct tccagcaggc caataacgtt
     8341 attgcggtta tcgtcagact tggacatctt cttggtcggc tccagcagcg acattacgcg
     8401 cgcgccagat ttcggaataa acggctccgg caccttaaag atatcgccgt acagcgcgtt
     8461 gaaacgctgg gcgatatcac ggctcagttc caggtgctgt ttctggtctt cacccaccgg
     8521 taccagatta gtttgataca gcaggatgtc cgctgccatc agcaccggat agtcaaacag
     8581 accagcgttg atgttctcgg cataacgcgc agatttatct ttaaactgcg tcatgcggct
     8641 cagctcgccg aagtaggtat agcagttcag tgcccagcct aactgcgcat gttccggtac
     8701 gtgggactga acaaaaatgg tgcttttctc aggatcgata ccacaagcca gatacaaggc
     8761 cagcgtatcc agcgtcgctt tacgcagctt ctgtgcatcc tggcgcacgg tgatcgcgtg
     8821 ttggtcaacg atacagtaaa tgcaatggta gtcatcctgc atgtttaccc actgacgcag
     8881 cgcacccatg tagttaccaa tggtcaattc acctgagggc tgtgcgccac taaaaacgat
     8941 gggcttagtc atttttcgat tcctgatttt cgctatgcgg aagccctaat gcgggcagaa
     9001 ggtcatttat agactgataa attacatcag gctggctgag atcgatagcc tcgccgtagt
     9061 tatatccgta ggttaagcca actgatgggc aacctgccgc ttttgctgcc tgaatatcat
     9121 tgcgtgagtc gccaacaaac agcatctgtt gtggagcaat tcccatccgc tcagccacca
     9181 gtaacagcgg gtccggatgc ggttttttgt tttgcacatc atcaccacca atcaccacgc
     9241 tgaagtattt ggcgatatct aaggcttcga gcagcggcgc gacgaacggc gtcggtttgt
     9301 tggtgaccag gcctagcggc aggcctttag cctgcaacgc gcccaacgta tcggcaacgt
     9361 gcgggaacaa aaacgtcccc tcttcggcaa cctcgccata atagcgatcg aacagtttac
     9421 gcagaatacg tacctgttct tctaccggaa tgtcgtcatc aacgggcggt ttacccattg
     9481 ttttacgcag agtcgcacgt tcctgacgcg cccaggccaa tgcacgctcc atcagaacat
     9541 ctgcgccgtt accaatccag gtaataacgc gttcttcacc tgcgacgggt aactccaacg
     9601 catacagcgc catatctacc gcagcagcaa gaccgggagc actgtccacc agcgtaccgt
     9661 cgagatcaaa agcgacgccg cgaatatctt caaacttact catgacttac ctttgccagt
     9721 tcactgcgca tttcatcaat gacttttttg tagtctggct ggtcgaagat tgccgaaccg
     9781 gcgacgaaca tatccgcgcc cgccgcagcg atttcgccaa tgttgttcac cttcacgcca
     9841 ccgtccactt ccagtcgaat gtcgaagcca gactcgtcga tacgacgacg tacttcgcgc
     9901 agtttatcca gtgtttgagg aatgaaagac tgaccgccga aaccagggtt gacggacatc
     9961 agcaggatca catccagctt atccatcacg taatccagat agctcagagg tgtcgccggg
    10021 ttaaatacca gacccgcttt acagccattt tctttaatca gttgcagcgt gcggtcaaca
    10081 tgctcggagg cttctggatg aaaggtaatg atgctggcac cagcggcagc gaaatcaggc
    10141 acaatgcgat cgacaggttt caccatcagg tgtacgtcga taggggcggt aatgccatag
    10201 ttacgcaagg atttcagcac cattggccca atcgtcagat tgggaacata gtggttatcc
    10261 atgacgtcaa aatgcacgac atcagcgcca gctgccaggg cttttgcggt atcttcaccc
    10321 aggcgggcaa aatcagccga cagaattgag ggggcaatca aatactgttt catccgcttc
    10381 tccttgagaa ttattttttc gcgggtgaaa cgactcctgg tttgtacaaa gccagcagtt
    10441 cgtccacctt tttacgtgtg ccgccgttgc tgcttatact gcgtcgaact ttgacgacat
    10501 gcaattttgc gcgctgatac cactcacgcg ttaacatcgt atcgtgattg gagatcagca
    10561 ctggaatatg gcgatcaacc agaccttcgg cgatctccgc cagatgcgct tgttgttcaa
    10621 gcgtaaaact gtttgtgtga tacgccgtaa agttggcggt cgcagacagc ggtgcataag
    10681 gcggatcgca atagacgaca gatgcatcat ctgcgcgcgc catgctatca gcgtaagact
    10741 cacaatagaa aaaggcattc tgcgcttttt cagcgaagtg atacaactct gcttccggga
    10801 aatagggttt tttgtagcgg ccaaacggca cattaaactc accgcgcaga ttgtaacgac
    10861 acaggccgtt gtaaccgtag cggttcaaat ataaaaacag taccgcccga cggaacggat
    10921 cctggctttt gttgaactct tcgcggaact gatagtaaac ctcggcgcaa tttgtttcgg
    10981 gaacaaacag ctcgcgtgcg gcctgtacgt actcatcagt acgcatcttc acaatgttat
    11041 agagactgat caggtcgcta ttgatatcgg caaggatata acgagaaaag tcggtgttga
    11101 gaaacaccga cccggcacct acaaaaggct caaccagaca ttcgcccttg ggcaaatgcc
    11161 gtttaatatc atcaagcagg ggatacttgc cccctgccca cttcaaaaaa gcgcgatttt
    11221 tcttcatgct gactaactaa ttacaccttc tccggctgtg gagaaagctc cgacagcatc
    11281 cagcgcttca ggcaactccc gcaacgataa accattgcgg gagataacct tgattacttc
    11341 agatcggcct gtacctgacg cagcggtttc gcccacgggt ttttggcctg aacatctgct
    11401 ggcaatgtag ataccgcttt tttcgcctct tctttcgaag cgtacacgcc agaaaccagg
    11461 acataccacg gctgaccatt acgcgtcgtt tcatagacaa cgtagttttt cagattctct
    11521 ttcttcgccc aaccgttcag gttgtcgtag ttagaggaac tgctcagctg cagagtgtaa
    11581 tggctggacg gtgccgattt caacgaacca acattacctg cggtcttccc tccagcggct
    11641 ggtgttgccg tggtttgcgc ctgggatgcc gtctgtactg gagccgtagt cgccgtctct
    11701 tttggtgctg gcgtagaagt cgcagccggt gccttcgtgc tcgccacagg ggcagctggt
    11761 tcagtacgct tcggcgtctg tgctaccggc ttcggttccg ttttcacggt tgcttgcggt
    11821 tttttcggtt caatcaccgc ctgctgacga gctggacgcg tagtggccgg acgttcagca
    11881 gtttgcgttt tcgccgtgtc acgcgatgca ttgccattgc gtactggcgc gacagttgct
    11941 ggttcagtcg gcaatgtaga attgaccgcc acattgttca actgttgctg attttgtggc
    12001 tgggtcagcg cattgttcag gtcaccctgc acttcaacac gttgttgacc atccgttgcc
    12061 gccggggttt gcccttgagt cggcgtagaa gagatcggcg gcagagaaac atcctgctga
    12121 gtattttccg cagacgtggt tcccggcgct ggctgcacgc cattcgcctg atcggtcgca
    12181 ttgccagcaa gatcaatact cttctcgcca gacgcggttt gatcgctgga agtggtcgag
    12241 ggggctttta gcgcagaacc gataccgatg atcaacagca gtagaaccag aatgccgacg
    12301 cccatcatca tatactgacg agaagcgggt ttactggctg cttttttgcg cttacgcgga
    12361 cgacgctcta cgcgctcttc atccacggtt tcatcttcgg attcgtcaat ttcttcttcg
    12421 atttccggtt cctcattgcg ctctttttgc gcacgagtcg gacggcgatc gtcagtgtca
    12481 tcaagttcaa tatcatcaaa attgatctgc ggttcgccac gttcagtacg ctcagaacgt
    12541 tcagaagatt gacgagaacg accagtacga cgatcgctgg gatcgggttt cagctcgtct
    12601 tctggtttga attcatccat ttaacacccc actaaaaggt taatgcttac cacgttgcaa
    12661 ttaacctgaa gctaatagac cgcttgataa gcggcctgac ctttcttgtt gttacgctga
    12721 ttgacaatcg gcaatggcgt taagaacaag ctcgtgcgaa acgccgctgc gaacttcact
    12781 cttaccaatt gccaacggaa gaattaagcg catctctccc gcaaggactt tcttgtcacg
    12841 cagcatatgc ggtaaatacg cctgcgcgga aatttcgcgc ggcccattga ccggtaaccc
    12901 agcccgcgtg agcagagtta taatacgctg cgtttcggca gaactaaact gcccgagacg
    12961 ttccgacgtc cgcgccgcca tcaccatacc cgcagcgacc gcttcaccat gtaaccaatt
    13021 gccatacccc atttcagctt caatggcatg accaaaggtg tgtcccagat tcagtaaagc
    13081 acgtaacccg gtttcgcgct cgtcggcggc gacaacttct gccttcagtt cacaacaacg
    13141 gcgaatacag tacgccattg ccggaccgtc cagacgcaac aacgcatcca gattctcttc
    13201 cagccagtta aaaaacgcac cgtcaagaat aatgccgtat ttgatgactt ctgccagccc
    13261 cgacgctaac tcacgcgggg gaagcgtttt cagacagtcg agatccacca ccactgaagc
    13321 aggctggtag aacgcgccaa tcatgttttt accgagggga tggttgaccg cagttttgcc
    13381 gccaacggag gaatcgacct gcgacagtaa cgtcgtcggg acttgaatga aacgaacacc
    13441 gcgctgataa ctcgccgccg cgaagccggt cagatcgccc actacgccgc cgccaagcgc
    13501 caccagcgta gtatcgcgac catgcggctt ttgtaacaac gccgtaaaga cggtatcgag
    13561 tacagccagg cttttatact gctcgccgtc aggaaggata acgctatcga cgttaacacc
    13621 cgcctgttca agtacgccgc ggaccttatc gagatacaga ggagccaggg tttcgttggt
    13681 gaccaacatg acctgctcgc ccgatttcag cggtaagaat gaagctggtt cattaaacaa
    13741 accagatgcg atggtaattg ggtaactacg ttccccgaga gtaacgacaa tcctctccat
    13801 aacgcgacat ccaccttaat tactgtaccc gcagacgagt gtatataaag ccagaattag
    13861 ttgctttcca gcatgtgaat aatctggtta gcgaccactt tagcgctttg atcatcagta
    13921 cgaatggtca cgtcggcaat ctcttcatac agcggattgc gttcattggc caacgcttcc
    13981 agaacttcac gtggcggtgt ttcaacgtgc agcaacgggc gttttttatc acgctgcgtg
    14041 cgtgcaagtt gcttttcgat ggtcgtttca agataaacga caacgccacg agcggaaaga
    14101 cggttacgcg tttcacggga tttcacagag ccgccgccag tagccagcac aataccctgt
    14161 ttctcggtca actcattgat gaccttttct tcgcgatcgc ggaagccttc ttcgccttct
    14221 aaatcgaaaa cccagcccac atcagctccg gttcgtttct caatctcttg atcggaatcg
    14281 taaaattcca tattgagttg ttgagctaac tggcgcccaa tagtgctttt tccggcaccc
    14341 ataggcccaa ccagaaagat attgcgtttc tctgccattt tttcggtact actaagacta
    14401 ttcgttaatg ataaacccgc ttcgctcaga gagcgccgca ggacatgaac tgaaacctca
    14461 taagatattg cgagagtcag actgaaaatt atctcaatac tccagcgggt ttggcaactg
    14521 aataaatcac ccatccctct gcatatctgg tcgctgcaag cgcgctgcct tgctaccacc
    14581 gctctggcga taaatcaccg ggtaagatta gcgtaaaaaa gacagcaaaa tgccgcctga
    14641 atgataaatc atcatcatgg aacgcaggca gcgaagagca ccaactctca aatcggtact
    14701 ccttgtatgc taaatacctg cgcgcgtcaa atagatgaaa cacgttcagc gtaaaaacat
    14761 taccgctttt acggctgttt actcactgga aaccagtcgt ggcgtgataa acaccactaa
    14821 ctcgcgtcgt tcatcttctt ttccgtcatg acgaaataat tgcccgaacc agggaatgtc
    14881 gccaagcaac ggcacgctat cctgacccga tttattttta cgggtaaaaa tgccgcccag
    14941 cgccaacgtt tctccgcttt tgacctcgac ctgcgtttcg atctcctgct tatcaatcgc
    15001 cagcacttcg ccatcggcct gctgtagcac ctgccccgga acgttctggc tgatgtgtaa
    15061 tttcagccgg atgcgacctt tttgtaacac cgtgggcgtg acctccatcc ccaggacggc
    15121 ctctttaaat tccaccgacg tcgcgccact ttccccgctg gaaacctgat atggaatttc
    15181 gctcccctgt ttaatgctgg caggctgaag atgtgaggcc agcagacgcg gactggcgat
    15241 aatatccagc tgctgttttt gttcgagcgc ggaaagctca agatccagca aacgtccgtt
    15301 gatgcgccca atgttaaaac cgacatgcgt tgtcgccgtc gctacggaga ggtcgctacc
    15361 aagcgtggtg acttgcccaa cgccaccagc gtgttgcgca tcggccagcg tccatttcac
    15421 gcctaactca cgcaaacttt tttcattaat ggtgacaata tgcgccgaca gctcaacctg
    15481 cccgaccggc agatccattt gcgctaccca ctgttcaagg gcgcttaacg ccgttttgtt
    15541 atctcgcagc aaaaggcgat tggtgcgttt atcgacggtc atactccctt tggcactcag
    15601 tagcttctcc cccgctttcg ccagctctcc ggcgtcggcg tattgcagag taatattgcg
    15661 attttccagc ggcagatttg cctgcgcccg cgcctgctcc gcctcctgac gggcgatatt
    15721 gtcattctgc caggcaacgg aatgcaccga gagaatgttg ccctcctggc gcgttatcag
    15781 tccggcgctt ttcactacag tttgtagtgc ctgcttccag ggaacatctg ttagatgtaa
    15841 cgacaccgta ccgctgacgt ctggcgacac caccaggttc aacttctcct gttcagccag
    15901 cgcctgcaac acctgagcta ccggaacgtc atccaccatc agcgtcactt tttgcggctt
    15961 tgccgcctgt acgccgggta tcagcatcaa cagtagtgcg gctatccatt gcttcatttg
    16021 tatctccttg ccgttgccac aaccattgtg gcggttcgca gtttgtcccg gtacccagcg
    16081 ttagtacgtc tggcgtcagc tgtaaaattg tccagccgtt ttccagcaca tcgttttgct
    16141 gcacccgtcg ccatttcttt tgcccgtctt ttattacacc gatgatgcgc tcgcctcgcc
    16201 ctaccatccc ctgatagcgc cactggctaa gttcgctaat ccggcataga tcttccggcg
    16261 gtttaaaagg gtcacgcata ccggttaaaa ggcacaatgc aatacctgcc aacaaccagc
    16321 gtttaaccct catgcggcgt ctccagttgt agcgtgaaca aaagatcatc accttccacg
    16381 cttaatgaaa aacagctcac gctgacgttg cgctctgcca gccgtgtaaa tgccgacggc
    16441 actgcttccc acagcgtttt caacgccaac tcgcctccct gcgcggatgg atgccagaaa
    16501 accagttgtg cgccgggcaa ctgaaaatcc agtggcgaaa agggcagcgt tttttcttct
    16561 ctgaagggag tggtgtctac caggcgatac agtgcggccc actgctggtg atgacttgcc
    16621 cgtagtcgaa ttaatgcgtc gctctcttca tggtgtgtcg atgacagaaa aatgagcgta
    16681 actaacatca gcaaccagac tgcccagcaa agctggcgga gacggggtga tgtggcgaac
    16741 caccagtcaa agaacatgtt catcgctaac cttccttgtt aactgatact caaattgcca
    16801 gcgtccctgc gcatcctgct gcgtggcccc ccgctgattg agatgaaaag acgcatcctg
    16861 gcggagtgac gtttctagtg cgtttaacgc ggtaatgctt gttgtcagcc ccttgatctc
    16921 cagcgttccc tgctgccagc ttatcgttgt cagccaggcg tgctcgggta aaagcgccgc
    16981 cagtgcttct agcgcagatt gccagtcgcg ggtaaattgt cgctggcgct gcctttgcaa
    17041 acgctgctca cgaatttgtt ggcgctccag caaacgtggt ttcgttatct gtaagctgcg
    17101 ggcgagttgt tgttccgcct gaagcaaaac ggcgtctatg cgcgcttcgg cgctgcttgt
    17161 cagacgcagt attagcgtta tcccgacggc cagcagcaga ggcgcaacga acatcagcaa
    17221 ccagaaacga agaaaagcgg tccggcgttg ctgtcgccag ggcaaaaaat taattggcgg
    17281 gttcatcagt acgtcttccc aagcgccagc cccagcgcga tggcaaagtc tccacctggc
    17341 ggcggtagcg gcggctgacg aacagaaacg gcctcccagg gatcaaatcc gccttcgcca
    17401 catatcgcga cgctttcggg atcaacggat aacgctgccg ccagctcctt cgcgctagtc
    17461 atccccaccg ccagtttgcg cccccagcga tagcgtgtcg cccacagcca ctgttcgtta
    17521 tcacgccagg ccagacattg ctgatgagaa ggtaaaaaag gcaggaatcg ctgtaatgca
    17581 ctggcatccg gggtgatcgc actaacatga acacgcaacc tttctgccag agtaagcagc
    17641 gttgccagct ctttgctttg cgcggcagtc acgttatagg cgggactgag tgagtcttcg
    17701 ctgtaatcga agcgcaggga gtccggatcc atatccagct cgcgagccat cgtcccggac
    17761 agccaggccg tttgctcccg ctcaccgagg gacatcgacg ggcgcggaaa tgaccgctgt
    17821 aatgtgcgac tggcgggaaa tgccaacatg atgtgagggc gctgcggcag ttcgcgactc
    17881 cacggtaaca acgttttagc cagccgctgc gcatcaacaa tccgcccatc tttgataatg
    17941 tcgtgcgcca gcggcaaccg ccaccagcgt tgcaaaaagc attcttttgc gccccgcacg
    18001 atagcaaccg ctaccgcttc ttgctgttgt aaatgcaaac caatttgcca gatcttaaat
    18061 gccattgtga tgatctcctt atcacccgtc actctgacgg gtatatcaat gcgtctggct
    18121 tgcctttata ctaccgcgcg tttgtttata aactgcccaa atgaaactaa atgggaaatt
    18181 tccagtgaag ttcgtaaagt attttttgat ccttgcagtc tgttgcattc tgctgggagc
    18241 aggctcgatt tatggcctat accgctacat cgagccacaa ctgccggatg tggcgacatt
    18301 aaaagatgtt cgcctgcaaa ttccgatgca gatttacagc gccgatggcg agctgattgc
    18361 tcaatacggt gagaaacgtc gtattccggt tacgctggat caaatcccgc cggagatggt
    18421 gaaagccttt atcgcgacgg aagacagccg cttctacgag catcacggcg ttgacccggt
    18481 ggggatcttc cgtgcggcaa gtgtggcgct gttctccggc cacgcgtcac aaggggcaag
    18541 taccattacc cagcagctgg cgagaaactt cttcctcagt ccagaacgca cgctgatgcg
    18601 taagattaag gaagtcttcc tcgcgattcg cattgaacag ttgctgacga aagacgaaat
    18661 cctcgagctt tatctgaaca agatttacct cggttaccgc gcctatggtg tcggtgctgc
    18721 ggcacaagtc tatttcggga aaacggtcga ccaactgacg ctgaacgaaa tggctgtgat
    18781 agccgggctg ccgaaagcgc cttccacctt caacccgctc tactcgatgg atcgtgccgt
    18841 cgcgcggcgt aacgtcgtgc tatcgcggat gctggatgaa gggtatatca cccaacaaca
    18901 gttcgatcag acacgcactg aggcgattaa cgctaactat cacgcgccgg agattgcttt
    18961 ttctgcgccg tacctgagcg aaatggtgcg ccaggagatg tataaccgtt atggcgaaag
    19021 tgcctatgaa gacggttatc gcatttacac caccatcacc cgcaaagtgc agcaggccgc
    19081 gcagcaggcg gtacgtaata acgtgctgga ctacgacatg cgccacggct atcgcggccc
    19141 ggcaaatgtg ctgtggaaag tgggcgagtc ggcgtgggat aacaacaaga ttaccgatac
    19201 gctgaaggcg ctgccgacct atggtccgct gctgcctgcc gcagtcacca gcgccaatcc
    19261 tcaggaagcg acggcgatgc tggcggacgg gtcgaccgtc gcattgagta tggaaggcgt
    19321 tcgctgggcg cgtccttacc gttcggatac tcagcaggga ccgacgccgc gtaaagtgac
    19381 cgatgttctg caaacgggtc agcaaatctg ggttcgtcag gttggcgatg catggtggct
    19441 ggcacaagtg ccggaagtga actcggcgct ggtgtcgatc aatccgcaaa atggtgccgt
    19501 tatggcgctg gtcggtggct ttgatttcaa tcagagcaag tttaaccgcg ccacccaggc
    19561 actgcgtcag gtgggttcca acatcaaacc gttcctctac accgcggcga tggataaagg
    19621 tctgacgctg gcaagtatgt tgaacgatgt gccaatttct cgctgggacg caggtgccgg
    19681 ctctgactgg cagccgaaga actcaccacc gctgtatgcc ggtccaattc gcttacgtca
    19741 ggggctgggt cagtcgaaaa acgtggtgat ggtacgcgca atgcgggcga tgggcgtcga
    19801 ctacgctgca gaatatctgc aacgcttcgg cttcccggcg caaaacattg tccacaccga
    19861 atcgctggcg ctgggttcag cgtccttcac cccaatgcag gtggcgcgtg gctacgcggt
    19921 catggcgaac ggcggcttcc tggtggaccc gtggtttatc agcaaaattg aaaacgatca
    19981 gggcggcgtg attttcgaag cgaaaccgaa agtagcttgc ccggaatgtg atattccggt
    20041 gatttacggt gatacgcaga aatcgaacgt gctggaaaat aacgatgttg aagatgtcgc
    20101 tatctcccgc gagcagcaga atgtttctgt accaatgccg cagctggagc aggcaaatca
    20161 ggcgttagtt gcgaagactg gcgcgcagga gtacgcaccg cacgtcatca acactccgct
    20221 ggcattcctg attaagagtg ctttgaacac caatatcttt ggtgagccag gctggcaggg
    20281 taccggctgg cgtgcaggtc gtgatttgca gcgtcgcgat atcggcggga aaaccggtac
    20341 cactaacagt tcgaaagatg cgtggttctc gggttacgga ccgggcgttg tgacctcggt
    20401 ctggattggc tttgatgatc accgtcgtaa tctcggtcat acaacggctt ccggagcgat
    20461 taaagatcag atctcaggtt acgaaggcgg tgccaagagt gcccagcctg catgggacgc
    20521 ttatatgaaa gccgttcttg aaggtgtgcc ggagcagccg ctgacgccgc cgccgggtat
    20581 tgtgacggtg aatatcgatc gcagcaccgg gcagttagct aatggtggca acagccgcga
    20641 agagtatttc atcgaaggta cgcagccgac acaacaggcg gtgcacgagg tgggaacgac
    20701 cattatcgat aatggcgagg cacaggaatt gttctgatta aaaaggcgct tcggcgcctt
    20761 tttaagtttg ctgacaaagt gcactttgtt tataccggat acggcgtgaa cactttaccc
    20821 gtctagcaac atctagaaaa atcaatatat tgcagaaact tcgtaagcct gataagcatc
    20881 aggcaaactt cacgcattta cactcgcccc tgccctttca accattcgcg cacgaggaac
    20941 agcgcgctga cattacgcgc ttcgttgaag tcagggtctt ccagcaaatc catcatatgc
    21001 gccagcggcc agcgcacctg tggtagcggc tctggctcat cgccttccag cgattccggg
    21061 tagagatctt gcgctaccac gatattcatt ttgctggaaa agtaagacgg tgccatgctg
    21121 agcttcttca aaaaagtcag atcgttcgca ccaaatccaa cctcttcttt tagctcgcgg
    21181 ttagctgctt cgtagacgct ttcacccgga tcaattaatc ctttcgaaaa acctaattcg
    21241 taggattcag ttcccactgc gtattcgcgg atcaggatca ggtgatcgtc cacaatcggc
    21301 acaatcatca ctgcttcccg gttggttgga cgcattcgtt cataaacacg ccgcacgcca
    21361 ttgctgaact ccagatccac gctctcgacg gtaaacagtc gggaacgggc tacagtttca
    21421 acattcagaa tggtgggttt ttgtaatgat ttgctcatcg tgggatctat gctgtgaaat
    21481 cagcggttat tgtgcgatat cggacacgct ttcggcaatg tgaattgcat gttatttaca
    21541 tttatgtaac ttaataaata atcgtcctca aatcaaatta aaagtcaata ggttgaaata
    21601 actccaggaa tttgctgata ttccgccttc agagggtttg ctatgatcag cggttactgt
    21661 gatgtgctta atgatgctca agttaaactc cacgcttgcc gatagccaac cgcagaatca
    21721 tgtattgtgt ccggtgcgac tgaccacgcc tgacagacta agtaagatgg ggaaagcatg
    21781 agcaccattg tgattttttt agctgctttg ctggcctgct cactacttgc gggatggctg
    21841 ataaaagtgc gatccagacg gcgtcagctg ccctggacca acgccttcgc ggatgcgcaa
    21901 acgcgtaaac tcacacctga agaacgtagc gccgttgaaa attatcttga gagcctgacg
    21961 caggtattac aggtgcctgg cccaacggga gccagcgcgg caccgatctc tctggcgctg
    22021 aatgccgaaa gcaacaacgt catgatgctg acacacgcta tcacacgtta cggcatctct
    22081 accgacgatc cgaataaatg gcgttactac ctcgattcgg tagaagtcca cctgcccccc
    22141 ttctgggaac agtacatcaa cgatgagaat accgttgaac tgattcatac cgattcgctg
    22201 ccgttggtta tttcactcaa cggtcatacg ctgcaggagt acatgcagga aacgcgcggc
    22261 tatgccttgc aacctgttcc gtccacgcag gcgtcgattc gcggggaaga gagtgagcaa
    22321 atcgagctac tcaatattcg caaagaaacg catgaagagt atgcgctgag tcgtccgcgc
    22381 gggctgcgtg aagcgttgct gatcgtcgcc tccttcctga tgttcttttt ctgcctaatt
    22441 accccggatg tatttgttcc gtggctggca ggtggcgcgt tactgctgct gggcgcaggt
    22501 ctgtgggggc tattcgcccc cccggcaaaa tcctccctgc gggaaattca ttgtctgcgc
    22561 ggtacacccc gtcgttgggg attgtttggc gaaaacgatc aggaacagat caacaatatt
    22621 tcactcggta ttatcgacct ggtctatccc gcacactggc agccatacat tgctcaggat
    22681 ctcggtcaac aaaccgatat tgatatctat ctcgaccgcc acgtagtgcg tcaggggcga
    22741 tatctttcgc tgcatgatga agtaaaaaac tttccgttac agcactggct gcgcagtacg
    22801 attatcgctg cgggctcgct gctggtgctg tttatgctgt tattctggat cccgctggat
    22861 atgccgctga aattcactct ctcatggatg aaaggcgcgc agaccattga agccaccagc
    22921 gtaaaacaac tggctgacgc tggcgtgcgg gtaggcgata cattgcgtat tagcggtacg
    22981 ggaatgtgta atattcgcac ttccggaacc tggagcgcga aaaccaattc acctttttta
    23041 ccgtttgact gctcgcagat catctggaac gacgcccgct cattgccatt accagaatct
    23101 gaactggtca acaaagcgac ggcattgact gaagcagtta atcgccagct gcaccctaaa
    23161 ccggaagatg aatctcgcgt cagtgcctca ttacgttcag cgattcaaaa atccggcatg
    23221 gtattgcttg atgattttgg cgacattgta ctgaagacag cggatctgtg ttctgccaaa
    23281 gatgactgtg tgcgactgaa aaatgcgctg gtcaatctcg gcaacagtaa agactgggac
    23341 gcgctggtaa aacgcgccaa cgccgggaag ctcgatggcg tgaatgtgtt attacgcccg
    23401 gtgagtgcgg aatcgctgga taaccttgtg gcaacctcca ccgcgccgtt tatcacgcat
    23461 gaaacggcgc gagcggcaca atcactaaac agtccggccc ccggcggatt cctgattgtc
    23521 agcgacgaag gcagcgattt tgttgatcag ccctggcctt cggcatcact ttacgactac
    23581 ccgccgcaag aacagtggaa cgctttccag aaactggcac aaatgctgat gcatacgccg
    23641 tttaacgccg agggtatcgt cacaaaaatc ttcactgacg ccaatggtac gcagcatata
    23701 ggccttcatc cgatcccgga tcgttccggc ctgtggcgct atctcagcac cacgctgctg
    23761 ctactgacga tgctgggtag cgccatttat aatggcgtac aggcctggcg gcgttaccag
    23821 cgtcatcgca ctcgcatgat gaagattcag gcctattatg aaagctgcct gaacccgcaa
    23881 ctgatcaccc cttcagaaag ccttatcgaa taacacgttt gcgcggcagg ttatgctacc
    23941 ctgtcgcgca aattgcttca ctctggagat ttccctcatg catatcaaca ttgcctggca
    24001 ggacgtagat accgttctgc tggatatgga cggcacgttg ctcgacctcg ccttcgataa
    24061 ctatttctgg caaaagctgg tgcctgaaac atggggcgcg aaaaacgggg ttacgccaca
    24121 ggaagcgatg gaatatatgc gccagcaata tcacgacgta cagcatacgc taaactggta
    24181 ctgtcttgat tactggagtg agcaactggg tctggatatc tgtgcgatga ccaccgagat
    24241 gggaccgcgt gccgtactgc gtgaagatac cattccgttt cttgaggcac tgaaagccag
    24301 cggtaagcag cgaattttgc tcaccaatgc gcatccgcac aacctggcgg taaaacttga
    24361 gcataccggt ctggacgcac accttgattt attactttcc acccacacat ttggttatcc
    24421 gaaagaggat cagcggttat ggcatgcggt ggccgaagct acgggtctga aagctgaaag
    24481 aacgctgttt attgatgaca gcgaagcgat tctcgatgct gccgcgcaat ttggtattcg
    24541 ttactgcctc ggcgtgacta atcctgactc agggattgcc gagaaacagt atcaacgcca
    24601 tccgtcactg aatgactacc gccgcctgat cccctcgcta atgtgaagga gacgccatga
    24661 aagaaaaacc tgctgttgag gttcgactgg ataaatggct atgggctgcc cgtttttata
    24721 aaacccgcgc gctggcccgt gaaatgattg aaggcggtaa ggtgcattac aacgggcagc
    24781 gcagcaagcc gagcaaaatc gtcgagctga atgccacgct cactctgcgc cagggaaatg
    24841 acgaacgcac ggtgattgta aaggcgatta ctgaacagcg tcgccccgcc agcgaggcag
    24901 ccttgctgta tgaagagact gcggaaagtg tagagaaacg cgaaaaaatg gcgctggcac
    24961 gtaaacttaa tgccttaacc atgccgcacc cggaccgacg cccggacaaa aaagagcgcc
    25021 gcgacctgtt acgatttaaa cacggcgaca gtgaataact gttacctgca agagagatga
    25081 ttatgccgca acatgaccaa ttacatcgct atctgtttga aaactttgcc gtgcgcggcg
    25141 aactggtaac cgtttcggaa accctgcaac agatccttga gaaccacgat tatccgcagc
    25201 ccgttaaaaa cgtgctggca gaactgctgg ttgcgaccag cctgttaacc gctacgctga
    25261 agtttgatgg tgatatcacc gtacagctgc agggggacgg tccgatgaat ctggcggtta
    25321 ttaacggtaa caataaccag caaatgcgcg gtgtggcgcg cgtgcagggc gaaattccag
    25381 aaaatgccga cctgaaaacg ctggtcggca atggttacgt ggtgatcacc attaccccga
    25441 gcgaaggcga acgctatcag ggcgtggtgg gtctggaagg agataccctg gcggcctgcc
    25501 tggaagatta ctttatgcgt tctgaacagc tgccgacgcg cctgtttatt cgcaccggcg
    25561 acgtagacgg caaaccggct gcgggcggta tgttgttgca ggtaatgcct gcgcaaaatg
    25621 cccagcagga cgacttcgac cacctggcga cgctaaccga aaccatcaaa accgaagaac
    25681 tgctgacctt accggcaaac gaagtgttgt ggcgtttgta tcacgaagaa gaggtgacgg
    25741 tttacgatcc gcaggatgtg gagttcaaat gcacctgctc gcgtgaacgt tgcgccgatg
    25801 cgctgaaaac gctgcctgat gaagaagtcg atagcatcct ggcggaagat ggcgaaattg
    25861 acatgcattg tgattactgc ggtaaccact atctgttcaa tgcgatggat attgctgaaa
    25921 tccgcaacaa cgcgtctccg gcagatccgc aagttcatta atgagtatgt ccggcagaga
    25981 gagctctgcc ggattttacg actaatacac ctgatgcgct ttgtcactca ttcagattca
    26041 catccagcct ttcaccatcc actacaagat cgcgccacgc tttaggcttc gttcccagtt
    26101 ttccatcctt cgcagctgtg agtaattttt ctttcgttat atcacgcgga agtaaactca
    26161 tcgtaagctc ttgccattct ttcttgtcag gatcaaagcc atagacaatg tatctttcat
    26221 catcaaaggc aaagaggatc cgctctgccc tgccatcact attcagatct tgctcgacca
    26281 gtacgcaggc gtctttttca atacaggtca tcacgttata gcgatcctgg attaaggccg
    26341 accagaatgt cgcatccggt ttaccagaac caggggcaat taacacattt tcggctaata
    26401 ctttttccga tatctgttgc tgaagatgtt gctctccatc taacgccatc aacagatccc
    26461 gcgcacgttt cgggtctttc ataaacccgg catcgctttt cagcgactca agcgcagcac
    26521 gtccatagct accgctctgt tcgagcatgt agatagttac ctggtctggc gtgtttttgc
    26581 cactctgata acgcgccata tgactgttca cactaatgcg catactgtcc agcaccggag
    26641 aattaagaag caccagtatt accaacacca ataacgaaac cgcaaggttc actttgccct
    26701 gaagagcaag gggattttgc cctttacgcc atacgatact gacaaaatac cccagcgacc
    26761 ataccagtaa caccagcacc gccaacgcgc cctgcaagcg gtcggcagtc cagccgtact
    26821 gagcgacccg cagccataac gcccaggcgg cgacgaacac atacagcgga gcaaccagca
    26881 aagcggtttt aatcaggcaa cgcaacggcc ccatccaggg aagagacgct ttttgcggat
    26941 cgcggacaat agccattaag atcaattgca aaaaggccag cgtcaacagc aacccggcgg
    27001 cggagatgtg gcgagaaatc gcgctcaggc ccgtaaacgg cagggtgatg ataaacatca
    27061 gggttagcaa tgatactaac ggcagcaacc ccgtggcgat taacgtgaac aacttttgaa
    27121 tagagtcgat taaacgtgac tgcgttcgcg cgaggatcac cgccagcgcg gtaaccagac
    27181 ctaacgtgag ataaataaac cagtcggttg caaaaaaaag cgttttgaaa aacgtgatgc
    27241 caacaagttt aaacaactcg ccccacagta aaagcaccag ccacgtcagg ccattggcga
    27301 gaaaaatcac cagtaatata agtatgttat gccatactga ctggtaaaag tagcggtagc
    27361 ggctactgtc attgcggata cgaaggcttt gttgtatcca cggtagcaac aacattgcca
    27421 tcagcagcag atagcaacca aaatcccaaa gcgccttttc agctctccag gggttcatgc
    27481 catcagtttg ccacttcagc cagccgctca tccccagcgt ggcaataaac accagcgcca
    27541 gccatcccca aaggcgcttc tgtttaaaag agatcacgga gaagagtaaa acggatgaaa
    27601 acgcgacggt cgcaggcacg ccataaacaa tccaactgtg attttttccg gaaagccagg
    27661 cgatcagcag gtagcaaacc agaccctgaa gtaatccggt agcaatcatc ccccagcgtg
    27721 ttgcgggtga aagttcaacg ttatccatgt tggttatcca gaatcaaaag gtgggttaat
    27781 tatcgcatcc gggcagtagt attttgcttt tttcagaaaa taatcaaaaa aagttagcgt
    27841 ggtgaatcga tactttaacg gttgaatttg catcaatttc attcaggaat gcgattccac
    27901 tcacaatatt cccgccatat aaaccaagat ttaacctttt gagaacattt tccacaccta
    27961 aaatgctatt tctgcgataa tagcaaccgt ttcgtgacag gaatcacgga gttttttgtc
    28021 aaatatgaat ttctccagat acgtaaatct atgagccttg tcgcggttaa cacccccaaa
    28081 aagactttac tattcaggca atacatattg gctaaggagc agtgaaatgc gcgttaacaa
    28141 tggtttgacc ccgcaagaac tcgaggctta tggtatcagt gacgtacatg atatcgttta
    28201 caacccaagc tacgacctgc tgtatcagga agagctcgat ccgagcctga caggttatga
    28261 gcgcggggtg ttaactaatc tgggtgccgt tgccgtcgat accgggatct tcaccggtcg
    28321 ttcaccaaaa gataagtata tcgtccgtga cgataccact cgcgatactt tctggtgggc
    28381 agacaaaggc aaaggtaaga acgataacaa acctctctct ccggaaacct ggcagcatct
    28441 gaaaggcctg gtgaccaaac agctttccgg caaacgtctg ttcgttgtcg acgccttctg
    28501 tggtgcgaac ccggatactc gtctttccgt ccgtttcatc accgaagtgg cctggcaggc
    28561 gcattttgtc aaaaacatgt ttattcgccc gagcgatgaa gagctggcag gtttcaaacc
    28621 agactttatc gttatgaacg gcgcgaagtg cactaacccg cagtggaaag aacagggtct
    28681 gaattccgaa aacttcgtgg cttttaacct gaccgagcgc atgcagctga ttggcggcac
    28741 ctggtatggc ggcgaaatga agaaagggat gttctcgatg atgaactacc tgctgccgct
    28801 gaaaggtatc gcttctatgc actgctccgc caacgttggc gagaaaggcg atgttgcggt
    28861 gttcttcggc ctttccggca ccggtaaaac caccctttcc accgacccga aacgtcgcct
    28921 gattggcgat gacgaacacg gctgggacga tgacggcgtg tttaacttcg aaggcggctg
    28981 ctacgcaaaa actatcaagc tgtcgaaaga agcggaacct gaaatctaca acgctatccg
    29041 tcgtgatgcg ctgctggaaa acgtcaccgt gcgtgaagat ggcactatcg actttgatga
    29101 tggttcaaaa accgagaaca cccgcgtttc ttatccgatc tatcacatcg aaaacatcgt
    29161 taagccggtg tccaaagcgg gccacgcaac gaaagttatc ttcctgaccg cagacgcctt
    29221 tggtgtgctg ccgccggttt ctcgtctgac tgccgatcaa acccagtacc acttcctctc
    29281 tggcttcacc gccaaactgg ctggtacaga gcgcggcatc accgaaccga cgccaacttt
    29341 ctccgcttgc ttcggcgcgg cgttcctgtc tctgcacccg acgcagtacg cagaagtgct
    29401 ggtgaaacgt atgcaggcgg cgggcgcgca ggcttatctg gttaacactg gctggaacgg
    29461 cactggcaaa cgtatctcga ttaaagatac ccgcgccatt atcgacgcca tcctcaacgg
    29521 ttcgctggat aatgcagaaa ccttcactct gccgatgttt aacctggcga tcccaaccga
    29581 actgccgggc gtagacacga agattctcga tccgcgtaac acctacgctt ccccggaaca
    29641 gtggcaggaa aaagccgaaa ccctggcgaa actgtttatc gacaactttg ataaatacac
    29701 cgacacccct gcgggtgccg cgctggtagc ggctggtccg aaactgtaat gatttgaagc
    29761 tggagaatat ctatccagta tcttatagaa agcaaaacgg gaggcacctt cgcctcccgt
    29821 ttatttaccc ttcttttgtc gtgccctgcg cccgcgttac cggcacaggc agccaggcgc
    29881 gaatggaaag cccgccccgc tcgctggtgc caagctccag catcccgtta tggttatcca
    29941 cgatacgctg cacaatcgcc agccctaatc ccgtgccgct aatggtgcgt gcactgtcgc
    30001 cgcgaacaaa cggctggaac aggtgcttgc gctgttctgg cgcaatcccc ggaccgtcat
    30061 cttctacctg gaaccaggcg cgattcggct ccgtcccgct gctgactttg atccagccat
    30121 tgccgtaacg ggcggcgttg accaccatat tcgccaccgc gcgtttgatc gacagcgggt
    30181 gcattttcac ttcaatgctg ccggggtaaa gcgcggtttc aatttcccgc tcatagccac
    30241 tttcggctgc aataacctca ccgagtactg cgttgagatc cgccatttcc atcggcatct
    30301 cctgcccggt gcgcaggtag tcgataaact gctcaatgat ggcgttgcac tcttcgatat
    30361 ctttattgat cgattcggcc aggtagccat cctgctcgct catcatctcg gtcgccaggc
    30421 gaatacgcgt cagcggcgtg cgtaagtcat gacttacccc cgccatcagc agcgtgcgat
    30481 catccgccag ttgcttaaca ccagccgcca tatggttaaa ggcgcgggta acggaacgca
    30541 cctcggacgc gccatactca cgcagcggcg gcggaataat ccctttaccg acctgcaaag
    30601 cagcatgttc gagatcgacc aacggtcggt tctggatacg aataaacagc cacgccccgc
    30661 ctatcgccaa tagcataatc gccaacgtat agcggaacag cggagagaaa tcgccctgat
    30721 gaatttcggt cagcggcacg cgtacccaga tattgggcga cagccaggtt ttcagccaga
    30781 cgacaggcga acttttgttg acctcaacgc gcacttccgt cgggccgccc agttgctgtg
    30841 ccatctgatg gcttaagaat tcatagtgtt gtgcccaacg cagacctgcc tcttcggcag
    30901 cttcgttgga gtagagagag atccccagct cacggtagat ctcccgacgg aaagcgggag
    30961 gcacaaccaa ctgcgtgccg tcctccagtt gcagtttgtc ggtcatcaac atacgcactt
    31021 cgtacgcgag gactttatta aactgctgga ggctcggcaa aatcgcgaag ttcagcacca
    31081 ccagataagt cgtcaccagg ctggcgaaca gcaaggtgac gatgagcaat aacgtacggg
    31141 caaatgaact tcgtggcgag aagcgcaatc gcctcatgct ttagagccgt ccggtacaaa
    31201 gacgtagccc aggccccaaa cggtctgaat gtaacgcgga tgcgctggat cttcttccac
    31261 catgcggcgc agacgcgaaa tctgcacgtc gatggagcgt tccattgcgg aatattcacg
    31321 accacgggca aggttcatca gcttatcgcg ggagagcggc tcacgcggat ggctgaccag
    31381 tgccttcagt accgcaaact caccgctggt gagcggcatc ggctcgtctt cgcggaacat
    31441 ttcgcgcgta ccgaggttaa gtttgaactt accgaaagca attaccgcct cttcctgtga
    31501 cggtgcgcct ggcagttcgt tcgcctgacg acgcagcacc gcacggatac gggccagcag
    31561 ttcacgcggg ttaaacggtt ttggaatgta atcgtcagcg ccaatctcca ggccaacgat
    31621 acggtccact tcttcccctt tcgccgtcac cataatgatc ggcatcgggt tgctctgact
    31681 acgaagacgt cggcaaatcg acaagccatc ttcaccaggt aacatcaaat ccagtaccat
    31741 aagatggaaa gattcacgag tcagcaggcg atccatctgt tctgcattag cgacgcttcg
    31801 aacctggaag ccttgttcgg tgagataacg ctccagcagc gcgcgcaggc gcatgtcgtc
    31861 atcgaccacc agaatcttgt agttctcttg cattgtttgt actcccaaag gttcgcaaca
    31921 atttgtaagc gtgtattctt aaaaaagctc acgttcgtca ccagctaaat ctggtatgaa
    31981 tttcagccta aattgttaca aagcatatta aacagcagct taagtataca atttattcgg
    32041 cgaaacatta ttgattctgt tgatatgatc acgttatacc caatgtgcgc attatcaaac
    32101 agacaaaggg aatcaacgag atgaaaacgc ccctggttac ccgggaaggg tatgaaaaac
    32161 tcaaacaaga gcttaattat ctctggcgtg aagaacgccc ggaggtcaca aaaaaggtga
    32221 cctgggccgc aagtctgggc gaccgcagcg aaaatgctga ctatcagtat aataaaaagc
    32281 gtctgcgtga aatcgaccgt cgcgtgcgct atctcactaa atgcatggaa aatctcaaaa
    32341 tcgtcgatta ctcccctcag caggaaggca aagtcttttt tggcgcgtgg gtggagattg
    32401 aaaacgacga tggcgtgact caccgtttcc gtattgtcgg ctacgatgaa atttttggcc
    32461 gtaaagatta catctctatc gattccccga tggcccgcgc attgctgaaa aaagaagtcg
    32521 gcgatctggc ggtggtgaat acacctgccg gggaagcgag ctggtatgtt aatgctatcg
    32581 agtacgtgaa accgtaagga agagtcttaa cctcctgccg atggctggca tttttgccag
    32641 ccagtccgta taactatccc ctgatttttg atccgaaaag atgaactcaa accatgatga
    32701 atgattcgtt ctgccgcatt attgcgggtg aaattcaggc gcgcccggaa caggttgacg
    32761 ctgccgttcg cctgcttgac gaagggaata ccgtgccttt tattgcacgt tatcgtaagg
    32821 aaatcaccgg cggtctggat gacacgcagc tgcgtaatct ggaaacgcgt ctgggatacc
    32881 tgcgcgagct ggaagagaga cgtcaggcga tcctcaagtc catttccgag caaggcaaac
    32941 tcaccgatga tctggcgaat gccatcaacg ccaccctaag caaaaccgaa ctcgaagacc
    33001 tctacctgcc ctacaaacct aaacgccgca cccgcgggca aatcgccatt gaagcagggc
    33061 ttgagccgtt ggctgacctg ctgtggagcg atccgtcaca cacgccagaa gtcgccgctg
    33121 cgcaatatat tgatgcagat aaaggcgtgg cagataccaa agccgcgctg gacggcgcgc
    33181 gctatatcct gatggaacgg tttgccgaag atgccgcgct gctggcgaaa gtgcgtgatt
    33241 atctgtggaa gaacgcgcat ttggtttcta cggtggtgag cggtaaagaa gaggaagggg
    33301 cgaaattccg cgactatttc gatcatcacg aaccattgtc cacggtgcct tctcaccgcg
    33361 cgctggcgat gttccgtggt cgtaacgaag gcgtactcca gctttcgctg aatgccgatc
    33421 cgcagttcga tgagccgcca aaagagagct attgcgagca aatcatcatg gatcatctcg
    33481 gcctgcgcct gaacaatgcc ccggcggata gctggcgcaa aggcgtggtg agctggacct
    33541 ggcgcatcaa ggtgctgatg catctggaaa ccgaactgat gggcaccgtg cgcgaacgcg
    33601 cggaagatga agcaatcaac gtctttgccc gtaacctgca cgatctgctg atggcggccc
    33661 ctgccggact gcgtgcaacg atgggcctcg atccgggtct gcgtactggg gtaaaagtgg
    33721 cggtggtcga tgccactggt aaactggtag cgaccgatac catttacccg cacaccggac
    33781 aagccgcaaa agcagcgatg actgttgctg cgctgtgtga aaagcataac gttgaactgg
    33841 tggcgatcgg caacggtaca gcttcccgcg aaaccgaacg tttctatctc gacgtgcaga
    33901 agcagttccc gaaagtgacc gcacagaaag tgatcgtcag cgaagctggc gcgtcggttt
    33961 actcggcttc cgagctggca gcgcaggagt tcccggatct cgacgtttcg ctgcgtggcg
    34021 cggtttctat cgcccgccgt ttgcaggatc cgctggcgga gctggtgaaa atcgatccga
    34081 aatctatcgg cgtaggtcag tatcagcatg acgtcagcca gacgcaactg gcccgcaaac
    34141 tggatgcagt agtagaagac tgcgtaaacg ccgttggcgt cgatctcaac accgcttctg
    34201 ttccgctatt aactcgcgtg gcgggcctga cgcgcatgat ggcgcaaaac atcgtggcct
    34261 ggcgcgatga gaacggccag ttccagaacc gccagcaact gctgaaagtc agccgtctgg
    34321 ggccgaaagc cttcgagcag tgcgcgggct tcctgcgcat taaccacggt gataacccgc
    34381 tggacgcctc taccgttcac ccggaagcct atccggtggt ggaacgcatt ctggcagcaa
    34441 cacagcaggc actgaaagat ctgatgggta acagcagcga actgcgtaac ctgaaagcgt
    34501 ctgactttac tgatgaaaaa ttcggtgtgc cgacagtaac tgacatcatc aaagagctgg
    34561 aaaaaccggg tcgcgatccg cgtccggaat ttaaaactgc tcagtttgcc gatggcgtcg
    34621 agacaatgaa cgacctgcaa ccgggtatga tcctcgaagg cgctgtcacc aacgtcacca
    34681 actttggcgc gtttgtcgat atcggcgtgc atcaggacgg cctggttcac atctcttcat
    34741 tgtcgaacaa gtttgtggaa gatccgcata ccgtggtgaa agcgggcgac attgtgaagg
    34801 tgaaagtgct ggaagtggat cttcagcgta aacgtatcgc cctgactatg cgtctggacg
    34861 agcagcctgg cgaaaccaac tctcgtcgcg gcggcggtaa cgatcgcccg caaaacaacc
    34921 gcccggcagc caaaccacgc ggtcgtgaag cgcagcctgc cggtaacagc gcgatgatgg
    34981 aagcgctggc ggcagcgatg ggtaaaaaac gttaaacgcc cggactggcc cctaaacctg
    35041 cgcgataagg cgttcacgcc gcatctggca acgaacgcct tgtctgacat aaaagtgccg
    35101 gagaatatct ccggcatttt tattccacag ccaaactcac aatatattcc ggcaacattt
    35161 atcacttcat taacaactga aaccttaatt aaacattagc cagtcctggt aattcgctat
    35221 tcgaattata ttttcgctgc gatataacct tgagccacat caacattgag tcagattatt
    35281 attcaaacca acattcgcac acattttaag tattgctgat agaaaccatt ctcattatca
    35341 ttgtactgtt gattatttaa tctctccttc gttggcaaat catctggtct catgtcgctg
    35401 tcaaacgccc catgaggtag ttatccagtt aatgagaaac aagtaggcac ctatgcaata
    35461 cactccagat actgcgtgga aaatcactgg cttttcccgt gaaatcagcc cggcatatcg
    35521 ccaaaaactg ctttctcttg gcatgttacc tggctcctct tttaatgtgg tgcgcgtcgc
    35581 tccactcggc gaccccattc atatcgaaac ccgtcgtgtg agcctggtat tacgcaaaaa
    35641 agatctggcc ttattagaag tggaagcggt ttcctgttaa tacggtgata acaacaatga
    35701 aaaaattaac cataggctta attggtaatc caaattctgg caagacaacc ttatttaacc
    35761 agctcactgg cgcacgtcag cgtgtaggta actgggctgg cgttaccgtc gaacgtaaag
    35821 aagggcaatt ctccaccacc gatcatcagg tcacgctggt ggacctgccc ggcacctatt
    35881 ctctgaccac catctcatcg cagacctcgc tcgatgagca aatcgcctgt cactacattt
    35941 tgagtggcga cgccgacctg ctgattaacg tggtggatgc gtctaacctt gagcgtaacc
    36001 tgtacctgac gctacaactg ctggaactcg gcattccctg catcgtggca ctgaacatgc
    36061 tcgacattgc cgagaagcaa aatattcgta ttgaaattga tgctctgtcg gcgcgtcttg
    36121 gctgcccagt gatcccgctg gtttcaaccc gtggtcgcgg tattgaagcg ctcaagctgg
    36181 cgattgatcg ctataaagct aacgagaatg tggaactggt gcattacgca cagccgctac
    36241 tcaacgaagc agattcactg gcaaaagtga tgccttccga catcccgctg aaacaacgtc
    36301 gctggctggg cctgcaaatg ctggaaggcg atatctacag ccgcgcctac gccggtgaag
    36361 cgtcgcagca tctggatgcc gccctcgccc gtctgcgtaa tgagatggac gatccggcgc
    36421 tgcacattgc cgatgcgcgt taccagtgca ttgctgccat ctgtgatgtg gtaagcaata
    36481 ccctgacggc agaacccagc cgtttcacca ctgcggtaga taaaatcgtg ctcaaccgtt
    36541 tcctcggtct gccgattttc ctctttgtga tgtacctgat gttcctgctg gctatcaaca
    36601 tcggcggggc gttacagccg ctgtttgacg tcggctccgt ggcgctattt gtgcatggta
    36661 ttcaatggat tggctacacg ctccacttcc cggactggct gactatcttc ctcgcccagg
    36721 gcctgggtgg cggcattaac accgtgctgc cactggtgcc gcagattggc atgatgtacc
    36781 tgttcctctc cttccttgag gactccgggt atatggcgcg tgcggcgttt gtgatggacc
    36841 gtctgatgca ggcgctgggc ttgccgggga aatcctttgt gccgctgatc gtcggtttcg
    36901 gttgtaacgt accgtcggta atgggtgcac gtacgcttga tgcaccgcgt gaacgtctga
    36961 tgaccatcat gatggcaccg tttatgtcct gcggcgcgcg tctggctatc ttcgcagtat
    37021 tcgcggctgc cttcttcggg cagaacggtg cgctggcggt cttctcgctg tatatgctgg
    37081 gtattgtgat ggcggtgctg actggcctga tgctcaagta caccatcatg cgcggtgaag
    37141 cgacgccgtt tgtcatggag ttgccggtct atcatgtacc acacgttaaa agcctgatta
    37201 tccagacctg gcagcgtctg aaaggcttcg ttctgcgtgc tggtaaagtg attatcatcg
    37261 tcagcatttt cctgagcgct ttcaacagct tctcgttgag cgggaaaatc gtcgataaca
    37321 tcaacgactc ggcgctggct tccgtcagcc gggtgatcac cccggtcttc aagccgattg
    37381 gcgtgcatga agataactgg caggcaacag ttggcctgtt tacgggtgca atggcgaaag
    37441 aagtggtggt gggtacgctc aacaccctct acaccgcaga aaatattcag gacgaagagt
    37501 tcaatccggc ggaatttaac ctcggtgaag agttgttcag cgcggtagat gaaacctggc
    37561 agagcctgaa agacaccttc agccttagcg tactgatgaa ccccattgaa gctagcaaag
    37621 gcgacggcga aatgggtacc ggggcgatgg gcgtgatgga tcagaaattc ggtagcgcag
    37681 ctgccgcata cagctacctg attttcgtcc tgctgtatgt accatgtatc tcggtgatgg
    37741 gagccatcgc ccgtgaatca agccgtggct ggatgggctt ctccatcctg tgggggctga
    37801 atatcgctta ctcactggca acattgttct atcaagtcgc cagctacagt cagcacccaa
    37861 cttacagcct ggtgtgcatt ctggcggtta tcctgtttaa catcgtggtt atcggtctgc
    37921 tgcgccgcgc gcgtagccgg gtggatatcg aactgctggc aacccgcaag tcggtaagta
    37981 gttgctgcgc agccagcacc accggtgatt gccattaatg gcttcactta ttcaggtgcg
    38041 tgatttgctg gcgttacggg gccgtatgga agcggcccag ataagccaga cattgaacac
    38101 tccacagcca atgattaacg ccatgctgca acaactggaa agtatgggca aagccgtgcg
    38161 gattcaggaa gaacctgacg gctgcctctc cggcagttgt aaaagctgcc cggaaggaaa
    38221 agcctgtctg cgcgagtggt gggcgctgcg ttaaccatac tccatcgcct gttttgaaaa
    38281 gcccggtatg cgtttgcatc cgggcttttt tgcgtgcggc tttccataaa aatgcaattc
    38341 ttgcagcgcg acgtaagttc ctctgaaagc gtctcgcagg gatgaaaact ggctaatgca
    38401 caggtgtgga gtggcgcgta gagtcgcggc attcaaacaa caggtgaagg aacgccatga
    38461 gcaaaaagca gagttccacc ccacacgatg cgctgttcaa actcttttta cgccaaccgg
    38521 agacggcacg cgattttctt gcgtttcatt taccggcacc cattcacgcg ctctgtgata
    38581 tgaaaaccct caagctggag tcgagcagct ttattgatga cgatctgcgt gaaagctatt
    38641 ccgatgtgct gtggtcggtg aaaacagaac aaggaccagg atacatctat tgtctgattg
    38701 aacatcaaag cacctcaaac aaactgatcg catttcgcat gatgcgttac gctattgccg
    38761 caatgcaaaa tcaccttgat gccggataca aaacgttgcc gatggtggtg ccattgttgt
    38821 tttaccacgg tattgaaagc ccctatccct attcgctgtg ttggctggat tgtttcgccg
    38881 atcccaaact ggcaaggcag ctttatgcct ctgcatttcc gctgattgat atcaccgtca
    38941 tgcctgatga tgaaatcatg cagcaccgac gcatggcgct gctggagtta attcaaaaac
    39001 atattcgtca acacgacctg ctggggctgg tagagcaaat ggcctgctta ttaagtagtg
    39061 gatacgctaa tgacagacaa atcaaagggc tgtttaatta catcttgcaa accggcgatg
    39121 cggtacgttt taacgatttt atcgacggcg ttgccgaacg ttcaccgaaa cacaaggaga
    39181 gtttaatgac tattgcggaa agattgcggc aggaagggga acaatccaaa gccctgtata
    39241 tagccaaaat aatgcttgaa tccggagtcc ctcttgcaga catcatgcgc tttaccgggc
    39301 tgtcagaaga agagttggct gcggcgagcc agtaaatttc tgtctcgcca ttcaaaagtg
    39361 cctacaccct ctgcttcaac gccaccagca cgtgacgaaa ctcgaccgga tgcgaaataa
    39421 acggcgcatg agccgctttg gcgaagatgt atgactcgct gtgtggccag agtttatcca
    39481 gcatcggcac cacttttcgc ggcaccagac catcgagata gccatacaat cgcaaaaacg
    39541 gcatggacac gttttgcagc ggctggcgga gatcgaccgt tttcaggatt tccagcccgc
    39601 cattaagcac gtcaacctcc ggcatcggta acgccagaac ggttttcttc aacgcccgcg
    39661 catcctgacg cgccgtttca gtacccatgg tttgtaacgc aaggaaccgt tccaccgtac
    39721 gctgaaaatc atcactgagt tgctgctgaa atcccgccag cacgtccggt tttatccccg
    39781 gccactcgtc acgagcacta aaacaaggtg acgacgccac ggtgaccagc gcctgaacac
    39841 gctcgggatg ggttaacgca atctggcttg ccaccagccc gcccagactc cagcctaacc
    39901 aaatggcttt atcaggtgcc tgtcgcagca cggcttcggc catatcagca agtgacatcg
    39961 caccaaatcc ccggctacgc ccgaagccgg gcaggtcaac aaggtgcagc gtaaaatgcg
    40021 agctcagttc ctcgtcaatg caacgccaca cttcggcatt cagtccccat ccgtgcagca
    40081 gcacaagatg aacattcccc tgacctttgg tctgccacca gatgttattc atccgctatt
    40141 gttctctttt gacttacaag gatgaacata tgctaacagt accaggatta tgctggctat
    40201 gccgaatgcc gctggcgtta ggtcattggg ggatttgttc ggtctgctca cgcgccgccc
    40261 gtacagataa aacgttatgc ccacaatgtg gattacccgc cacacattca catcttccct
    40321 gcggtcgctg cctgcaaaaa ccaccgccct ggcaaagact ggtcacggtt gccgactatg
    40381 cgccgccgtt aagcccgcta atccaccagc ttaagttttc ccggcgcagc gaaatcgcca
    40441 gcgccctgtc acgtctgtta ctattggaag tcttacacgc tcgtcgcacc accgggttgc
    40501 aattgccgga tcgtatcatc agcgttccgt tatggcagcg gcgtcactgg cgtcggggat
    40561 ttaatcagag cgatttgctg tgtcagccgt tatcacgctg gttgcactgc caatgggata
    40621 gcgaagccat cacacgaaca cgggccactg cgacccagca ttttctcagt gcccggctac
    40681 gcaagcgcaa cctgaaaaat gcctttcgtc ttgaattgcc cgtgcaaggt cgccatatgg
    40741 tgattgtgga tgatgtcgtt accaccggaa gtaccgtcgc agagattgcg cagttgcttt
    40801 tacgcaatgg tgcggcgact gtccaggtct ggtgcctttg tcgaaccttg tagagcctcg
    40861 atgatgggcg tattataacc aactaaaata gtcaactatt aggccattac tatgatccgt
    40921 atttccgatg ctgcacaagc gcactttgcc aaactgctgg caaaccagga agaagggaca
    40981 caaatccgcg tatttgtgat taaccctggc acgcctaacg ctgaatgtgg tgtttcttat
    41041 tgtccgccgg atgccgtgga agccaccgac actgccctga aatttgacct gctgaccgcg
    41101 tatgttgatg agttaagcgc tccttacctg gaagatgcag agatcgattt tgttaccgac
    41161 cagttgggtt ctcagctaac actgaaagcg cctaacgcca aaatgcgtaa agtggcagac
    41221 gatgcaccac tgatggagcg cgtggagtat atgctgcagt cgcagatcaa cccacagctt
    41281 gctggtcacg gtggccgcgt ttcactgatg gaaatcaccg aagacggtta cgccattctg
    41341 caatttggcg gcggctgtaa cggttgttcc atggtcgatg tgacgctgaa agaagggatc
    41401 gagaagcagc tgctaaacga attcccggag ctgaaaggtg tgcgcgatct caccgaacac
    41461 cagcgcggcg aacactccta ctactaagtt atcccctctt ttgtagattg cccgatgcga
    41521 cgctaaagcg tcatttcggg cctacgttga acatgtgcca gacgttggcg cagcattgct
    41581 ttggcaaatc cctcccttcg tttccccgcg ataatatgac caacctctca taatttaaat
    41641 ttaccccgct ctggtgattc tcaaacgcca gatgttaccc gtatcattca catgggtacc
    41701 aaacatactc ctgacatctg actacaataa ttagttttag tgggtatcag tcgtggtgcc
    41761 gcaatatctc tgttcccgat tgggataatt agagtttgtc gtcagaaaat tgacgttacc
    41821 cataacaaat gaaaggccag gtaaatcatg ccattagtca ttgttgctat cggtgtaatc
    41881 ttgttgttgc tcctgatgat ccgcttcaaa atgaacggct tcatcgctct cgtcctcgtg
    41941 gcgcttgctg ttggattaat gcaaggaatg ccgctggata aagttattgg ctccatcaaa
    42001 gccggtgtcg gcgggacgct cggtagcctt gccctgatca tgggttttgg cgcaatgctg
    42061 ggcaaaatgc tggcagactg cggtggcgca caacgtatcg ccaccacgct gattgccaaa
    42121 tttggtaaaa aacacatcca gtgggcggtg gtactgaccg gttttaccgt tggttttgcc
    42181 ctgttctatg aagtgggctt tgtgctgatg ctgccgctgg tgtttaccat cgcggcttct
    42241 gcgaatattc cactgctgta tgttggtgta ccaatggcgg ctgcactgtc tgtgacccac
    42301 ggcttcctgc caccgcatcc gggcccgact gcgattgcca ccattttcaa tgccgacatg
    42361 ggtaaaaccc tgctgtacgg tactattctg gcaatcccga ccgtgattct cgccggtccg
    42421 gtttacgctc gcgtgttgaa aggtatcgat aagccaatcc cggaaggtct ctacagcgcg
    42481 aaaaccttca gcgaagaaga gatgccgagc tttggcgtca gcgtctggac ctctctggtg
    42541 ccggtagtac tgatggcgat gcgtgcgatt gccgaaatga tcctgccgaa aggtcacgct
    42601 ttcctgccgg tagcggagtt cctcggtgac ccggtaatgg ctacgctgat tgccgtgctg
    42661 attgcgatgt tcacctttgg tctaaaccgt ggtcgttcaa tggatcagat taacgacacg
    42721 ctggtttctt ccatcaaaat cattgcgatg atgctgttga tcatcggtgg tggcggtgcg
    42781 ttcaagcagg tgctggtaga cagcggcgtg gacaaataca ttgcttccat gatgcacgaa
    42841 accaacattt ctccgctgct gatggcctgg tcgattgctg ccgtactgcg tatcgcgctg
    42901 ggttccgcaa ccgttgcggc aatcactgcg ggtggtatcg cggcaccgct gattgcaacg
    42961 acgggtgtta gcccggagct aatggttatt gcggtgggtt ccggtagtgt gattttctct
    43021 cacgtgaacg atccaggctt ctggctgttc aaagagtact ttaacttgac tatcggcgag
    43081 accatcaagt cctggtcgat gctggaaacc attatctcgg tgtgcggtct ggtaggctgt
    43141 ctgctgctga atatggtgat ttgataacac tgactgccgg atgcatcgca tccggcatca
    43201 accgcactct acttcttctt cgctgcagct ctgcgccgtc tgtccaaatc cttcagcaac
    43261 ttgttcacgc catcatcggc aaacatcgtc tcaagcgttg cggaaagctt gcgtcgccag
    43321 tttttatact ggtaactggt gccaggaata ttcaccggtt cggccatatc cagccagtct
    43381 tccggctgta gtcctaacag agcactgtta ctgtcggcaa tgtagcgctg caaaccacgg
    43441 ttcagcgtcg gcgtcatcga catcaacgat gccttatgcc cggcacgttt tggcagacaa
    43501 ccatatttat gcagtgcatc cagcagccct tgcttcgcca gttcgcgatc ctgatacaga
    43561 ccgcgcagta ccacttcatc cggatacagc cccagggttt tgcccagcgt tagatccccg
    43621 ctctcccagt agccgcgtag cgtaggcagg tcatgtgtcg ccgcaaccgc catcgactgc
    43681 tccggatacg ctttcggtgc acggaacgtc ttctcgtggt cgttttcgaa atagagcact
    43741 ttgtaggagt acacaccgct gctgcgcagc ttaccgacaa tctctaccgg tacggtaccg
    43801 agatcttcac caatcaccat acagcgatga cgtttacttt cgagtgccag aatcgagagc
    43861 agatcatcca ccggatagtg aacatacgcg ccctgatctg ccgtctcgcc atacggtatc
    43921 caccacaaac gcagcatcga catcacatgg tcaattcgta atgcgccgca gttttgcata
    43981 ttggcacgca acagctcgat aaacggttca taggcacgcg cggtgatgat atgcgggtcc
    44041 attggcggta atccccagtt ctgccccaac gggccgagga tatccggcgg cgcgccaacc
    44101 gacgctttca ggcaatatag ttcacggtca caccaggttt ccgccccacc ttccgctacg
    44161 ccaaccgcca gatcacgata caagccaatc ggcatttcat agccctggct tatctcccag
    44221 caggcggcaa actggctgta agccagccac tgcaaccaga gataaaaatc gacgtcatta
    44281 cgatgttctt cgcagaactg acgcacttct ggtgaatcca cgttctgata catctctggc
    44341 catgcaggcc agccccagcg catttcgtcc tctttcactt gctgggcatg tagcgcatca
    44401 aaggctgcct gccagaacag gctgtcgccc tgctctgcaa caaactggcg aaacgcggcc
    44461 atctgctcat catcacgttg cgcgaaacct ttccacgcca ttcgtaatgc tgtcattttt
    44521 agggcggtaa ccgtggagta atcgacccag tcggcatcgc gcgcctgttg cagcgtctgt
    44581 tgcgtggtcg gcaactgcca ccaggcctga gcctcttcgc taagatggaa atcttcaacg
    44641 gcgttaacgt cgatataaat cacattcagc caacggcgag aagacgggct gtatgggctg
    44701 gcactctccg gatttgccgg atagagcgca tgaatcgggt tcaggccgat gaacgacccg
    44761 ccacgttttg ccacatccac cagcatcgct ttgagatcgc caaaatcccc aataccccag
    44821 tttttttccg atcgcagcgt ataaagctga acgcaggcac cccacagctt ttgtttattc
    44881 agcaacgcct gcggttcgta acagcgtttc ggggcgacaa tcacccggca atgcgcgcgc
    44941 tggtcgtcct gggtgagtgt cagcgtgtga taaccttccg gcaacttcgt cggtagattg
    45001 aacgctttgc cccccgttac atggcctttg tactgtgttc cttcttcggt ggtcagcagc
    45061 cagctatatt cgccgctgcc ctccaccacc atcggcattt ttttgccgct ggtataaacc
    45121 atgacattcg ggactggcgt taccgccact ttcgtggcgg tacgttgatg catcgcgtca
    45181 agcaaacgcc gtttggtttc ggcgctaatc gactgcggtt taccgtgggc attgatgtaa
    45241 ttggggctaa tccccgccgc cagcgcggca ttatccagac gtttgctttc catcgagctt
    45301 ccttagcgtt ttgcctgcca gatacgagcc tgataatcgc gaatagagcg atccgagcta
    45361 aacataccgc agcgggcggt attgaggatc gccgcgcgag tccaggcctc ctggtcgcgg
    45421 tacagcacat ccacctgctt ttgtgcctca acataggctg cgaaatccgc catcaccaga
    45481 tacggatcgc cgccttgttt gccgatactg tgcagcatct ggtcgaaggc atgcttatcg
    45541 ccgtcgctgt atttaccgct ttccagctct ttcaatactg cgtccagcac cttatctttc
    45601 ttccgccatt tcaccgggtc gtagcctttg gccagaattg ccttcacttg ttccacggta
    45661 tgaccaaaaa taaagatatt ttcttcaccg actttctcgg cgatttcaac gttcgcacca
    45721 tccagcgtac cgacagtaag cgcaccattg agcgccagtt tcatattgcc ggtaccggaa
    45781 gcttctttac ctgcagtcga aatttgttcg gagatatccg ccgccgggat cagtttttcc
    45841 gccgccgaaa cgcaataatc cggcaggaac accaccttca acttatcgcc aaccagtgga
    45901 tcgttgttga tcacgtcagc cactttgttg atcgcaaaga taatattctt cgccaggtag
    45961 tagcccggtg ccgctttcgc gccgaagagg aagacgcgcg gtacgcgatc agcctgcggg
    46021 ttttcacgaa tttctttgta caacgccaga atatgcagca gattcaggtg ctggcgtttg
    46081 tactcatgca gacgtttgat ctgaatatcg aaaatcgcct gtggattgat ctcaataccg
    46141 gtacgaactt tcacaaactc cgccagacgg actttattcg cctgcttgat ctcgcgatat
    46201 tgctgacgga atttcgcatc atcagcgaat ttttccagat tgatcagctg atcgagatcg
    46261 ttagcccact ctttttgcag tgatttatcc aacagagccg ccagtgccgg gttgcactgt
    46321 ttgatccagc gacgtggggt aataccgttg gtgacgttat ggaatttgtt cggccatagc
    46381 tggtgatatt ccgggaacag atctttcacc accagatccg agtgcagcgc cgcaacaccg
    46441 ttcaccgcga aaccgccaac cacacacagg ttcgccatat gcacttgttt gtcgtgcacc
    46501 accgccagtt tggcccacac tttttcatcg cccggccagg ttttctctac cagcgtttta
    46561 aagcgagtat taatttcgtt aataatctgc atgtggcgcg gcagtaagcc tttcaccagt
    46621 ttcacatccc agcgttccag cgcttctggc atcagggtat ggttggtgta agcgaaagtt
    46681 ttgctggtaa tggcccaggc gtcatcccag ctcatctggt gctcatcgat cagcacgcgc
    46741 agcagttctg gaatcgcgat agttgggtgg gtatcgttca gctgaataac ttcgtaatcc
    46801 gccagttcgt gcagtttacg ccccgccaga tgatggcgac gcaaaatatc cgctaccgaa
    46861 caggcacact ggaagtattg ctgcatcagg cgcagctttt taccggcagt atggttgtcg
    46921 tttggataga gaactttggt cagtttttcc gcattgatgc cctgctgttc ggcacgcaag
    46981 aaatcaccgt cgttaaattt agtcagatca aacggatgcg cgtgcgtcgc ctgccacaga
    47041 cgcagcggct gcgccacgcc attacgatag ccgacaacgg ggagatccca cgcttgaccg
    47101 gtaatggtaa actccggctc ccagcgtccg tctttcgtca ctttaccgcc aatccctacc
    47161 tgcacatcca gtgcttcgtt gtggcggaac cacgggtagt tactgcgatg ccagtcatcc
    47221 ggcgcttcaa cctgtttgcc atcgacaaaa gactggcgga acaaaccata ttgatagttc
    47281 agaccgtaac ccgtcgcaga ctgaccgaca gttgccattg agtcgaggaa gcacgccgcc
    47341 agacgtccca gaccaccgtt acccagcgcc gggtcgatct cttcttccag caggtccgtc
    47401 agattgatgt cataagcctt caacgaatcc tgtacatcct gataccagcc gagattcaac
    47461 aggttgttgc ccgtcaggcg accaatcaaa aactccattg agatgtagtt aacatgtcgc
    47521 tgattcgcca ccggcttggc gaatggctga gcacgcagca tttcggccag tgcttcactc
    47581 actgctagcc accactggcg aggagtcatt tcagccgcag aatttaagcc ataacgctgc
    47641 cactgacgtg aaagcgcttc ctgaaattgc ttatcgttaa aaataggttg tgacatagga
    47701 gttccacttt tcttagattt tcaacacaac gttatcgcta gtttgccagg ctcgatgttg
    47761 accttcctca tcctgcgggg gattaggcag ggaggagttg cggggatgag caaggaaatg
    47821 tgatctcaac cacttaaagc tagtgcaaac cacaggatta gcatcaaatc aatgcaatac
    47881 agcgcagaaa atctgtatct aagtgcaaaa aatggccgtt gcgtattttc aaaaagcgga
    47941 aggtaactct ataaattaag taaaggagtg aaacagtttc ataagtaaaa tatccagtgt
//
LOCUS       DAPGOF010000012        47746 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.012, whole genome shotgun sequence.
ACCESSION   DAPGOF010000012 DAPGOF010000000
VERSION     DAPGOF010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47746
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.012"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(108..371)
                     /gene="cedA"
                     /locus_tag="RHV13_000587"
     CDS             complement(108..371)
                     /gene="cedA"
                     /locus_tag="RHV13_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HDV1240147.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            554..2815
                     /gene="katE"
                     /locus_tag="RHV13_000588"
     CDS             554..2815
                     /gene="katE"
                     /locus_tag="RHV13_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HDV1240148.1"
                     /translation="MSQQNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MEIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(2862..3620)
                     /gene="chbG"
                     /locus_tag="RHV13_000589"
     CDS             complement(2862..3620)
                     /gene="chbG"
                     /locus_tag="RHV13_000589"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HDV1240149.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSHGFSSAFYGEEISEALFLQVLDDSSHRGERSLEVMCHPAFVDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(3633..4985)
                     /gene="celF"
                     /locus_tag="RHV13_000590"
                     /gene_synonym="chbF"
     CDS             complement(3633..4985)
                     /gene="celF"
                     /locus_tag="RHV13_000590"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDV1240150.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQIVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCTLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5089..5931)
                     /gene="chbR"
                     /locus_tag="RHV13_000591"
     CDS             complement(5089..5931)
                     /gene="chbR"
                     /locus_tag="RHV13_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HDV1240151.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(5939..6289)
                     /gene="chbA"
                     /locus_tag="RHV13_000592"
     CDS             complement(5939..6289)
                     /gene="chbA"
                     /locus_tag="RHV13_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HDV1240152.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6340..7698)
                     /gene="chbC"
                     /locus_tag="RHV13_000593"
     CDS             complement(6340..7698)
                     /gene="chbC"
                     /locus_tag="RHV13_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HDV1240153.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            complement(7783..8103)
                     /gene="chbB"
                     /locus_tag="RHV13_000594"
     CDS             complement(7783..8103)
                     /gene="chbB"
                     /locus_tag="RHV13_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HDV1240154.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(8403..8741)
                     /gene="osmE"
                     /locus_tag="RHV13_000595"
     CDS             complement(8403..8741)
                     /gene="osmE"
                     /locus_tag="RHV13_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HDV1240155.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            8943..9770
                     /gene="nadE"
                     /locus_tag="RHV13_000596"
     CDS             8943..9770
                     /gene="nadE"
                     /locus_tag="RHV13_000596"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HDV1240156.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLRTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            10000..10887
                     /gene="cho"
                     /locus_tag="RHV13_000597"
     CDS             10000..10887
                     /gene="cho"
                     /locus_tag="RHV13_000597"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HDV1240157.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(10847..11422)
                     /gene="ves"
                     /locus_tag="RHV13_000598"
     CDS             complement(10847..11422)
                     /gene="ves"
                     /locus_tag="RHV13_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HDV1240158.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADRFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(11625..12110)
                     /gene="spy"
                     /locus_tag="RHV13_000599"
     CDS             complement(11625..12110)
                     /gene="spy"
                     /locus_tag="RHV13_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HDV1240159.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(12440..13408)
                     /gene="astE"
                     /locus_tag="RHV13_000600"
     CDS             complement(12440..13408)
                     /gene="astE"
                     /locus_tag="RHV13_000600"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HDV1240160.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(13401..14744)
                     /gene="astB"
                     /locus_tag="RHV13_000601"
     CDS             complement(13401..14744)
                     /gene="astB"
                     /locus_tag="RHV13_000601"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HDV1240161.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNKASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVTRLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMLLVLPQECREHAGVWRY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLMNLGSVYPFQREGGGNG"
     gene            complement(14741..16219)
                     /gene="astD"
                     /locus_tag="RHV13_000602"
     CDS             complement(14741..16219)
                     /gene="astD"
                     /locus_tag="RHV13_000602"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HDV1240162.1"
                     /translation="MTLWINGDWITGQGALRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAAFPRWARLSFAERQAVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVIAW
                     QELEAMGGRTLLAPRLLQAGTSLLTPGIIEMMGVAGVPDEEVFGPLLRVWRYDTFDEA
                     IRMANNTRFGLSCGLVSPEREKFEQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(16216..17250)
                     /gene="astA"
                     /locus_tag="RHV13_000603"
     CDS             complement(16216..17250)
                     /gene="astA"
                     /locus_tag="RHV13_000603"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HDV1240163.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(17247..18467)
                     /gene="astC"
                     /locus_tag="RHV13_000604"
     CDS             complement(17247..18467)
                     /gene="astC"
                     /locus_tag="RHV13_000604"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HDV1240164.1"
                     /translation="MSQPITRENFDEWIIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECASVMTVG
                     THGTTYGGNPLASAVAGKVLDLINMPEMLNGVKQRHDWFVERLNIINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAVACEHFVSRGSS"
     gene            18913..19719
                     /gene="xthA"
                     /locus_tag="RHV13_000605"
     CDS             18913..19719
                     /gene="xthA"
                     /locus_tag="RHV13_000605"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDV1240165.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSPLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            19886..20596
                     /locus_tag="RHV13_000606"
     CDS             19886..20596
                     /locus_tag="RHV13_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDV1240166.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTRIAFWP
                     YTLVSALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRNPLTHPKNEG"
     gene            20601..21278
                     /gene="ydjY"
                     /locus_tag="RHV13_000607"
     CDS             20601..21278
                     /gene="ydjY"
                     /locus_tag="RHV13_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HDV1240167.1"
                     /translation="MLQHYSVSWKKGLTALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKTYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            21293..22000
                     /locus_tag="RHV13_000608"
     CDS             21293..22000
                     /locus_tag="RHV13_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDV1240168.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHDFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALSV
                     VIFMAKKIWLERQKRNA"
     gene            22000..22548
                     /locus_tag="RHV13_000609"
     CDS             22000..22548
                     /locus_tag="RHV13_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDV1240169.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQNPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            22558..23724
                     /locus_tag="RHV13_000610"
     CDS             22558..23724
                     /locus_tag="RHV13_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDV1240170.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTDWAETLPNWRYVDTQLPVQEDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPNDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            23697..25232
                     /locus_tag="RHV13_000611"
     CDS             23697..25232
                     /locus_tag="RHV13_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV1240171.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWSGPKWQRMCTRLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDKLGIGLGFTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLGYLLWCTWRRTIPRVDGVRKP
                     ATPLLPGITLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALQLVAGQYTLALWLNMDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLTIFALTALVAKWVGYVRQ
                     GLR"
     gene            25232..25885
                     /locus_tag="RHV13_000612"
     CDS             25232..25885
                     /locus_tag="RHV13_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDV1240172.1"
                     /translation="MLYVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAKRHIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            25952..27259
                     /gene="ynjE"
                     /locus_tag="RHV13_000613"
     CDS             25952..27259
                     /gene="ynjE"
                     /locus_tag="RHV13_000613"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HDV1240173.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNGKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(27268..27888)
                     /locus_tag="RHV13_000614"
     CDS             complement(27268..27888)
                     /locus_tag="RHV13_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDV1240174.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKLLQRQ"
     gene            27975..28382
                     /gene="nudG"
                     /locus_tag="RHV13_000615"
     CDS             27975..28382
                     /gene="nudG"
                     /locus_tag="RHV13_000615"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HDV1240175.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELREELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            complement(28348..28620)
                     /locus_tag="RHV13_000616"
     CDS             complement(28348..28620)
                     /locus_tag="RHV13_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HDV1240176.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            28856..30199
                     /gene="gdhA"
                     /locus_tag="RHV13_000617"
     CDS             28856..30199
                     /gene="gdhA"
                     /locus_tag="RHV13_000617"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HDV1240177.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(30316..31356)
                     /locus_tag="RHV13_000618"
     CDS             complement(30316..31356)
                     /locus_tag="RHV13_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HDV1240178.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEICNSNFERMIKENKAMLLCKWGG
                     YLTCAVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLEDQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANHSSSKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(31483..33444)
                     /gene="topB"
                     /locus_tag="RHV13_000619"
     CDS             complement(31483..33444)
                     /gene="topB"
                     /locus_tag="RHV13_000619"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDV1240179.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPAEERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            complement(33449..34492)
                     /gene="selD"
                     /locus_tag="RHV13_000620"
     CDS             complement(33449..34492)
                     /gene="selD"
                     /locus_tag="RHV13_000620"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HDV1240180.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(34609..35160)
                     /locus_tag="RHV13_000621"
     CDS             complement(34609..35160)
                     /locus_tag="RHV13_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HDV1240181.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            35321..37177
                     /gene="sppA"
                     /locus_tag="RHV13_000622"
     CDS             35321..37177
                     /gene="sppA"
                     /locus_tag="RHV13_000622"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDV1240182.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLAEVSITRALPPEAQLMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            37331..38347
                     /gene="ansA"
                     /locus_tag="RHV13_000623"
     CDS             37331..38347
                     /gene="ansA"
                     /locus_tag="RHV13_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDV1240183.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            38358..38999
                     /gene="pncA"
                     /locus_tag="RHV13_000624"
     CDS             38358..38999
                     /gene="pncA"
                     /locus_tag="RHV13_000624"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HDV1240184.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(39160..39432)
                     /locus_tag="RHV13_000625"
     CDS             complement(39160..39432)
                     /locus_tag="RHV13_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HDV1240185.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARNNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(39474..39887)
                     /gene="msrB"
                     /locus_tag="RHV13_000626"
     CDS             complement(39474..39887)
                     /gene="msrB"
                     /locus_tag="RHV13_000626"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDV1240186.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            40229..41224
                     /gene="gapA"
                     /locus_tag="RHV13_000627"
     CDS             40229..41224
                     /gene="gapA"
                     /locus_tag="RHV13_000627"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDV1240187.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            41308..42192
                     /locus_tag="RHV13_000628"
     CDS             41308..42192
                     /locus_tag="RHV13_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HDV1240188.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASEMQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(42243..43097)
                     /gene="yeaE"
                     /locus_tag="RHV13_000629"
     CDS             complement(42243..43097)
                     /gene="yeaE"
                     /locus_tag="RHV13_000629"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HDV1240189.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASKCKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISTAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELAMLDKAYPAPK
                     GKTALDMV"
     gene            complement(43187..43933)
                     /gene="mipA"
                     /locus_tag="RHV13_000630"
     CDS             complement(43187..43933)
                     /gene="mipA"
                     /locus_tag="RHV13_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HDV1240190.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            44369..46303
                     /gene="yeaG"
                     /locus_tag="RHV13_000631"
     CDS             44369..46303
                     /gene="yeaG"
                     /locus_tag="RHV13_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HDV1240191.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            46416..47699
                     /locus_tag="RHV13_000632"
     CDS             46416..47699
                     /locus_tag="RHV13_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HDV1240192.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
BASE COUNT        11786 a        12500 c        11761 g        11699 t
ORIGIN      
        1 gatcgtcaaa ggcagctttt tgttacaggt ggtttgaatg aatgtagcaa tgaaatacag
       61 aatttcaggc catgtaactc ccggcaaaac cgggaggtac gtagtcctta ctcagtcact
      121 tccccttcct ggcggatctg atttgcccaa cgctgggcgg attcaggcac agtaaacgcc
      181 ggtgagcgca gaaatgactc tcccatcagt acaaacgcaa catatttgcc acgcagcatc
      241 cagacatcac gaaacgaatc catctttatc gcatgttctg gcggcgcggg ttccgtgcgt
      301 gggacatagc taataatctg gcggttttgc tggcggagcg gtttcttcat tactggcttc
      361 actaaacgca tattaaaaat cagaaaaact gtagtttagc cgatttagcc cctgtacgtc
      421 ccgctttgcg tgtatttcat aacaccgttt ccagaatagt ctccgaagtg ggatctggct
      481 ggtggtctat agttagagag ttttttgacc aaaacagcgg ccctttcagt aataaattaa
      541 ggagacgagt tcaatgtcgc aacaaaacga aaagaaccca catcagcacc agtcaccact
      601 acacgattcc agcgaagcga aaccggggat ggactcactg gcacctgagg acggatctca
      661 tcgtccagcg gctgaaccaa caccgccagg tgcacaacct accgccccag ggagcctgaa
      721 agcccctgat acgcgtaacg aaaaacttaa ttctctggaa gacgtacgca aaggcagtga
      781 aaattatgcg ctgaccacta atcagggcgt gcgcatcgcc gacgatcaaa actcactgcg
      841 tgccggtagc cgtggtccaa cgctgctgga agattttatt ctgcgcgaga aaatcaccca
      901 ctttgaccat gagcgcatcc cggaacgtat tgttcatgca cgtggatcag cagctcacgg
      961 ttatttccag ccatataaaa gcttaagcga tatcaccaaa gcggatttcc tctcagatcc
     1021 gaacaaaatc accccagtat ttgttaggtt ctctaccgtt cagggtggtg ctggctctgc
     1081 cgataccgtg cgtgatatcc gtggctttgc caccaaattc tataccgaag agggtatttt
     1141 tgaccttgtt ggcaataaca cgccaatctt ctttatccag gatgcgcata aattccccga
     1201 ttttgttcat gcggtaaaac cagaaccaca ctgggcaatt ccacaagggc aaagcgccca
     1261 cgatactttc tgggattatg tttctctgca acctgaaact ctgcacaacg tgatgtgggc
     1321 gatgtcggat cgcggcatcc cccgcagtta ccgcaccatg gaaggcttcg gtattcacac
     1381 cttccgcctg attaatgccg aagggaaggc aacgtttgta cgtttccact ggaaaccact
     1441 ggcaggtaaa gcctcactcg tttgggatga agcacaaaaa ctaaccggac gtgacccgga
     1501 cttccaccgc cgcgagttgt gggaagccat tgaagcaggc gattttccgg aatacgaact
     1561 gggcttccag ttgattcctg aagaagatga attcaagttc gacttcgatc ttctcgatcc
     1621 aaccaaactt atcccggaag aactggtgcc cgttcagcgt gtcggcaaaa tggtgctcaa
     1681 tcgcaacccg gataacttct ttgctgaaaa cgaacaggcg gctttccatc ctgggcatat
     1741 cgtgccggga ctggacttca ccaacgatcc gctgttgcag ggacgtttgt tctcctatac
     1801 cgatacacaa atcagtcgtc ttggtgggcc gaatttccat gagattccga ttaaccgtcc
     1861 gacctgccct taccacaact tccagcgtga tggcatgcat cgtatggaga tcgacactaa
     1921 cccggcgaat tacgaaccga actcgatcaa cgataactgg ccgcgcgaaa caccgccggg
     1981 accgaaacgc ggcggttttg aatcatatca ggaacgcgtg gaaggaaata aagttcgcga
     2041 gcgcagtcca tcttttggcg aatattattc ccatccgcgt ctgttctggc taagtcagac
     2101 gccattcgag cagcgccata ttgtcgatgg tttcagtttt gagttaagca aagtcgttcg
     2161 tccgtatatt cgtgagcgcg ttgttgacca gctggcgcat attgatctca ctctggccca
     2221 ggcggtggcg aaaaatctcg gtatcgaact gactgacgac cagctgaata tcaccccacc
     2281 tccggacgtc aacggtctga aaaaggatcc atccttaagt ttgtacgcca ttcctgacgg
     2341 tgatgtgaaa ggtcgcgtgg tagcgatttt acttaatgat gaagtgagat cggcagacct
     2401 tctggccatt ctcaaggccc tgaaggccaa aggcgttcat gccaaactgc tctactcccg
     2461 aatgggtgaa gtgactgcgg atgacggtac ggtgttgcct atagccgcta cctttgccgg
     2521 tgcgccttcg ctgacggtcg atgcggtcat tgtcccttgc ggcaatatcg cggatatcgc
     2581 tgacaacggc gatgccaact actacctgat ggaagcctac aaacacctta aaccgattgc
     2641 gctggcggga gacgcgcgca agtttaaagc aacaatcaag gtcgctgacc agggtgaaga
     2701 agggattgtg gaagctgaca gcgccgacgg tagttttatg gatgaactgt taacgctgat
     2761 ggcagcacac cgcgtgtggt cacgcattcc taagattgac aaaattccgg cgtaaatatg
     2821 gataaggtca cgtcccctgg ggagtgacct taccacctta cttacacatc aagataacta
     2881 cccaaccgat aaccacgctc agcaattgca tatttcaacg acgctgatgt cagcacttcc
     2941 agctccgtta accgtgggaa acagtaagca ctctggcgaa tcgtattatc gacaaacgcc
     3001 ggatggcaca tgacttccag cgagcgttcc ccgcgatggc tggaatcatc aagcacttgc
     3061 aggaacagtg cctcgctaat ctcttcacca taaaacgcgc tactgaatcc gtgcgaactg
     3121 cgcagattag ccggtaaatc accagcgtta gatagcggct gacggtcgat gcgcaacgca
     3181 atcccctctt cagccgcaaa cctggcaaca atagggaaaa tctgcgggaa catatgcaca
     3241 tgatgatggc tatcaagatg cgtaggtttg cgtccaaata gctcaatgaa acgcaaatac
     3301 tgactggcaa gctcctgagt aatttcttcc agcggtaaag catcttcttc cgccaactgc
     3361 cagatccatt ttcccagcac accatcgcgg gttaaccccg gcatagctgt cagtggcttg
     3421 cccatagtaa ggacaaagtg catccctatg gccagactcg gttcatcacg actcaactgc
     3481 accgcatggt caatagcctg cccattcacc agcgccgtcg tcgatgtgac aatcccattg
     3541 cgacaggcct cgataatgcc gtagttctga cctttgctta agccaaaatc atcggcatta
     3601 acaatcagta agcgttccat aatcagcctc ggttaatgtg cttttttaag ctctgcgatg
     3661 cagtcggcaa aatttggcag ccatttctcg tgcgccagaa tcatctcgcg tgccagcagc
     3721 tcagcatcgc gatcggaatg caccaacgga ctaaggttta gcgccagtaa cacatcgtta
     3781 aattctccgc taagtgcggc gttgctggca gcaatctcga agcctttaat ggtgtgaatc
     3841 agccccatca ctttatcatc gaaatgcgta atgcgcggat gtggcgtcgc gccatcgcgc
     3901 cccagcgtac aggtcatttc taccgcccag tctgccggaa tattatcaat atgcccatga
     3961 tgcgggatat taacgtaatg ttctgcttgc ttgtcgttgt agatagcgtt gatcacttcg
     4021 cacgctgcat cagagtaata agccccaccg cgctgttcca gttctttcgg cttaactttc
     4081 aactccgggt ttttatacag ctcaaaaagt tgtttctcga ctttctgtac tatctgcgct
     4141 cgtgcgccgc ctttgtagta ttcgcccatt tcaatagcca gcatctcttt ttgcttgaag
     4201 taataaagca aataagaaca cggcaacaga ttcagagaac gaattaagcc ttcgctaaat
     4261 ggcagatcga aaatattttt aacgccagat gcttttaact gcccggatgc cacaccatca
     4321 agtaattcgg caaagcgaga tttgccattt accagcacat ccttaatgaa caccatatgg
     4381 ttgaggccga acagatcgat agataaatca tcgctgtctt tcagcatcag aacatcgcga
     4441 ataaacatct tcatgccgat cggaatatta cacacgccga taaagcgttt aaatccggta
     4501 tgacgataaa cggcttcagt gaccattccc gccgggttag tgaagttaat cacccatgca
     4561 ttcggacaaa gttcttcgac atcttttacg atgtcaaaaa tcaccggaat ggtacgcaga
     4621 cctttaaaca gaccgcccgc gccgttggtt tcctgaccaa gataaccatg acttaatgga
     4681 atacgttcat ccagttcgcg cgccggtaat tggcctacgc gcagttgggt agtaacgaaa
     4741 tcagcatctt tcaatgcttc gcggcgatcc agcgttttat aaagcttcat cgggacgcca
     4801 gcgttatcaa tcatccgttg gcagagatcg aaaataatat ccagtttagc tttgccacct
     4861 tcgacatcca ccagccataa ttcgctgacc ggcaattcgt gataacgctt aataaatcct
     4921 tccagtaact ccggggtata gctgctcccg ccaccaatag tgacgacttt taatttctgg
     4981 ctcataattt ctcccttcag tacagaatac tgatatctgg catgtctgcc ccccggacat
     5041 aaataatcca gcaacaggac agatatgtga attgtcaggt ataacgactt actgattaaa
     5101 ttcagtcaat ttcttacgat agctcttagg cgtaaaggac gttaatttct taaacgtttt
     5161 aataaacaaa ctcgggctac tataaccggc ctcaaacgca atatccgtca ctgaatagtt
     5221 ggtcatttcc agttgttttt tggcaaaatt aatacggatt tcattaataa tctgcattgg
     5281 cgttttgcca taatatcgtt gagtcgctcg cgtcaaatat tcctgtgatt tggctgacaa
     5341 cgtcaccata ttctccagcg ccgattcact aaactgctct ttatcatgca tcttttctac
     5401 cgtacttttc agccactgcg gtacatcatc aatcacctgt tcttcgcggt aatgacgtaa
     5461 acggttaatg acataaaaag taaccatctc aacaaactct tccagccctg tttcgcggaa
     5521 attcaatgaa gaaatcactg tttccacata ggtgagaaaa gcgttattgg tccggtatac
     5581 ctgcgaagct acaaagcaat aaggcaacaa tggcaggtaa tgctgctcaa aaaagcgttt
     5641 actgatccca acgttcaata tgcgcgtggc accaaactca taaaaacttt gatggtgcga
     5701 acctaacgga ataaaaacaa aatcgccccg ttccagtaac acgcgcttac cgttaatctc
     5761 ctggaaataa cgcccggtta ataccagagt aaattcataa tagtcgtgct gatgcagtcc
     5821 gctgatactc tcagttttgt tatagataaa cacatggaag tttttgccat taaacaactg
     5881 ctgttctcgg gcagtggcaa tttccggcgc gttaatcact ggctgcatca tcgactcctt
     5941 atgccttcag tttttcatga agctcaatta attcagtaat cagttcacgc gcaagcatgg
     6001 acgtcattaa atgatcctga gcgtggacca gcaccagact cactttcatc ttaccttcgc
     6061 ccgcatcgcc ttcaatcagt ttcgtctgta ccagatgcgc ttcattcaat gccatccgtg
     6121 actgatccat catggctttt gctgcggcaa aatcgccctg cttcgcctgt ttcagtgccg
     6181 catacgccag gctgcgcgct tgtccggagt tgatgataag ccccatcacc acttcttcca
     6241 gctcttcagc ttccgtttgc gtatcgggaa tattatcgag atccatcata catcgttcct
     6301 ctctattctt accggcacga ttacccgtac cggcatcgat taaaatttca gggcgttagc
     6361 gatatcttct tcgctctctt ctttatcaat cgcgttttgt gctttgttag ccaccacaac
     6421 aaagggcaga taaattaacg ttgcgatacc aaggttgaag agtgcaacca gcaatgcggc
     6481 gacgctaccg ttggtgttaa agaaggctcc cagaccggtt ggcatggtcc acggtgcaat
     6541 attggtcacc ggaggaataa tgcccatgta gtacgctgcc agggtgattg ccgccagaat
     6601 cggttgtacc agtacaaacg ggataaacat caccgggttc atgataattg gcagaccaaa
     6661 cagaatcggt tcgttaatct ggaagatgcc ggacggcagc gccagttttg ccacctgacg
     6721 ataatcagca cggcgagagg cgataaagat agccaggatc aggcctaaag tcgcaccact
     6781 gcccccaagg aaaataaagg agtccagcat cggcttggcc cagatatgga aggtcttacc
     6841 ggctgccagc gccgcttcga cggaaccata ttgctgatag gtcgcgatat tttccagcgc
     6901 ccacggcgtc ataatgccgt tgtccagtgc ggtcagcgcc agcgcgccat gaataccgaa
     6961 gaaccagagc agtggaacaa agatcacata ggcccagccc accacgctac ccaacgatgc
     7021 cagtggggtt gagatggtat ccataatgat ctgatggaag ttggtgcccc aggtattcaa
     7081 cgcccaggca ataatcccca tcacggaaag aataataaag ccgggaatta atgccgagaa
     7141 ggaacgcgat actgaagcag gtacgctgtc gggcagttta atgacccaat tgcggcggac
     7201 aataaaggta aacatttctg ccaccaccag gccaataata atcccggaga tgatattcgc
     7261 cccacctaac cagtttgcgc caaccgcata ggcctcaccg acactatatg gggtgacggt
     7321 cataaatgct gcaacggata acaacccagc cgccagcgca tcgactttac gctcttctgc
     7381 cagcgccatg ccaataaaga acggtgccat taaagacatt atccctaatg ttccgttata
     7441 tacgttgccg ccaatacctt tcagaccatt aagtgtttca atggttgagg cgtcgaggcg
     7501 aatacctaag gaataaaaaa acgacccctc cccaaagctt agaaaaacgt tgttaattaa
     7561 tacaaacatg gcccccgcaa gggttaacgg cattaagcga ataaagccat ttttgattgc
     7621 attaacgtgt ggctgctttc ctattttaac tgcaaaaggg aggagtacct tttcaagcga
     7681 tgcaataaca ttactcatag aaaaataccc ttaaaaaccg caatttaaat attgcggtat
     7741 tgatttatga aataactctt tgacgggaaa atttaaaata aattaatttg ctgcggcttt
     7801 tttaatcgct gcaaccgcag ccttaagcac gcctaaacca tcgactttgc cataaagcag
     7861 cgagtcaatt acttcaaccg gtttgttggg taacaaacgc tggatttcgg gcaacatata
     7921 agcaatctgc ggccctaata acacgacatc ggcattctga cctttttcac cagccagtgt
     7981 ttccggaaat gcttcaataa tgaccggaac ttcatatttt tctgcctgtg cgcgcatttt
     8041 tgataccagt aaagaggtag acatgcccgc agaacaaaac agataaatgt gtttcttttc
     8101 cataaaactg ccctcatcga cgattatctg tcagccagac actccgcaag ccttaacctg
     8161 cttccatgct ctgggtaact tgcgaaacca acatgacttt ttttgtggct gaaaggagta
     8221 tacggtatag acctgagcaa tagtatttct ttgaccttat aaattgtctc tccttgacct
     8281 gcggttatga ccctcttcac atttcgggca aataattcgc gaagataatt aagaactcag
     8341 gccaaaaaaa aaccggcgca atggccggtt tccgttgtta ctcaagcttt cagacgaagt
     8401 gattacttcg cagcctgtgg atcagtgtcg tattcagcac aggtctgata accggagttg
     8461 atgacatgtc cggtatcatc taacgcgaca aagtaggttt ctgctttacc atcacgttga
     8521 cccaggatgt aggtctggca agtaccacga gcatggatca tactcacttc agacgaaggt
     8581 ttacccgcaa tttgtgcaac ctgcgcccgg ctcatgcctt ttttgacgtc tttcaccaca
     8641 ggctgtacaa actggtcttt ggtacgatca taagccgtac aacccgccag catggttaat
     8701 accgccgctg cactcagaat tcctgccata ttcttgttca tattccgtcc tcttgtttat
     8761 cagcgtgtta gataagcctg gaatacattg ggcgcttttt caagcccatg aacgaaacgg
     8821 ctccgctttc agaggattcc tgtatgacgt tttaaccacc attcagcccg ctgtcgcttg
     8881 tcgtttcagt agcaacgggt tagctttaag gaagttttgt cttttctgtc tggaggggtt
     8941 caatgacatt gcaacaacaa ataataaagg cgctgggcgc aaaaccgcag attaatgctg
     9001 aagaggaaat tcgtcgtagt gtcgattttc tgaaaagcta cctgcgaact tatccgttca
     9061 ttaaatcact ggtgctcggg atcagcggcg gtcaggactc cacgcttgcc ggtaagctgt
     9121 gccagatggc gattaatgag ctgcgccagg aaaccggcaa cgaatcactg caatttattg
     9181 ccgtacgcct gccctatggt gttcaggccg acgaacaaga ttgccaggat gccattgcct
     9241 ttattcaacc ggatcgcgta ttaaccgtta atatcaaggg cgcggtattg gctagcgagc
     9301 aggcattgcg agaagcaggc attgaactga gcgattttgt ccgtggcaat gaaaaagcgc
     9361 gtgagcggat gaaagcacaa tatagcattg cgggtatgac cagcggtgtc gtggtgggca
     9421 ccgatcatgc agcagaagcc attaccggat tcttcactaa atatggtgac ggcggtacgg
     9481 acattaatcc actgtatcgt ctcaacaaac gtcagggtaa acagttactg gcagcattag
     9541 gttgcccgga acacctttat aagaaagcgc caacggccga tctggaagat gatcgccctt
     9601 ctctgccaga tgaagtggca ctcggcgtga cctatgacaa tattgacgac tatctggaag
     9661 ggaaaaacgt acctgaacag gtcgccagaa caatagagaa ctggtatctg aaaaccgaac
     9721 ataaacgccg tccgccaatt accgttttcg atgatttctg gaaaaagtaa taatttgcac
     9781 ctattggatt gtgcgaaaaa gagtaatttg ttcacgccgg atgcggcgtg aacgccttat
     9841 tcgacctaca aaactatgca aattcaatac attgcaggag tcgaataggc ctgacaggcg
     9901 tagcacgtca gacggtgtaa cctttgtcat cgacccgcct cttttttaat cgcttcccgc
     9961 ctgttacact ggatagataa ccagcattcg gagtcaacag tggtacggcg tttaacttct
    10021 ccgcggctcg aatttgaagc tgcggcaatt tatgaatatc ccgaacattt acgttcattc
    10081 cttaatgact tacccacccg accaggggtg tatctgtttc atggcgaaag tgacaccatg
    10141 ccgctctata tcggcaaaag cgttaatatc cgcagtcggg tgctttctca tttacgcacc
    10201 cctgatgaag ccgccatgct acggcaatcc cggcgaatta gctggatctg caccgcaggc
    10261 gaaatcggcg ctctgctcct tgaagcgcga ttaatcaaag aacaacagcc gctgtttaat
    10321 aaacggttgc gccgcaatcg ccagctctgt gccctgcaat taaatgaaaa gcgcgtcgat
    10381 gtggtgtatg ccaaagaggt ggatttttca cgagccccca acctgtttgg cctgtttgcc
    10441 aataggcgcg cagctttgca agcattgcag accatcgctg atgaacaaaa actttgttat
    10501 ggcctgttgg gactggaacc gttaagtcgc ggtcgtgcat gttttcgttc agcgctaaaa
    10561 cgttgcgccg gagcatgctg cggtaaagag agccatgagg aacatgcgct acgcttgcgc
    10621 cagtctctgg agcgtttgcg ggtggtgtgt tggccttggc aaggtgcggt ggcactgaaa
    10681 gaacagcacc cggaaatgac tcaatatcat attattcaaa actggctgtg gctgggggcg
    10741 gttaattcgc tggaagaagc gacaacgtta attcggacac ccgccgggtt tgatcacgac
    10801 ggttataaaa ttctttgtaa gccgctgctt tccggtaact atgaaattac tgaacttgat
    10861 ccggcgaatg accagcgagc cagttgattt cactgaacaa cagtttacct tgcggttgta
    10921 aaagccgcag ggtgtgcctg ccatcgaacc agcacgcgcc ctggtcggta gtgagcagtt
    10981 tgtcgcccag ttgccacgcc ccgttaatca caaacaccac cccgccgcgt gagccaaagg
    11041 tagtaaacgt acgttcggca attctgactt tcgccttaca gacatccagg cgcgtcataa
    11101 tattgaaatc catcgacatc tgccctgccg tcagtttcgc ttttaccacc tggtccgccg
    11161 caaaggcaaa aggctgtaac ggttttaagg tatggttaaa gcggtctgcg ctttcaagga
    11221 acatctcgcc gccttccagc aacgtcacta tcctttccat accgggaaac aaagaaaact
    11281 ccccattcgc cgcgatggat gcaatgctgg cacgccagta aaaatcacgt tttgccggtg
    11341 ggaaagtgca aatttctcgc gtttcaccgg cagcgtttcg ccacagattt accgacattt
    11401 tacgcatatc aaagtattcc atactcgctc ccggcccgtt cttttcattg ttatcgttca
    11461 ggtaaccgtg gatggttatc ggcaactcgg gtgatttacg ttagtggtga tcaggctttt
    11521 tttacaactg tttgagaaga gaaaagaaaa ccgccgatcc tgtccaccgc attactgcaa
    11581 ggtagtggac aagaccggcg gtcttaagtt ttttggctga aagattattc agcagttgca
    11641 ggcattttac cttttgccgc tggacgttct gtcagacgct tctcaaaatt agcattaaat
    11701 tgctttttct gttccggcgt caggatgttg taaattttgt tctgggtttc catgtgcgcc
    11761 agcatgttag ctttgcgctg ttcttccatt tttgcgatct gcgcttcagc ttttgcttta
    11821 tcgaaggtat cgctggcaat gatgtcatgc attgcgcggc gttcttccag cggcggacgt
    11881 ttcatctggt cacgctggcc tttcatgatt tcgcggatct gctgtttctg cgcgtcggtc
    11941 aggttcaggt ctttgaacat catgtcctga tgcggaccga acttgccttt gtggtgcatc
    12001 atcggcttcg cgtcagccgg tgctgcggta gtggtgtctg cggcatgggc caggttagcc
    12061 gcgccaagag ccagggtaga ggcaacaaac agtgcagtta atttacgcat attctatatc
    12121 cttcctttca gttatttatt acggctttct taagtagcgt gccgtgttga cgagattaac
    12181 tttactgact ttagcgtcaa ttaatcagag caacggtaaa acaatgaaag tgtaaaaaac
    12241 acttttgcgc caattatgga gaaaaaaaga aaatttgatg gagagtgatg agcgaatatt
    12301 acaacacgat gattttgcag agattatgaa gaactatacc ggatgactgg tgataaataa
    12361 agcaaataac caggattaat ctgtattaat ttataagaaa gtaacttaat acccgcagaa
    12421 tgatttctgc gggtaagtat tagcttattt tttcgagcat taatcccgcg cgtaatccca
    12481 acgctaccaa aggattaggg aataacacat actctacatc atgggttacg gtaaaacgtt
    12541 cctccccgtc ctgcgccagc aatgttcctt tctcaaacgg cataaaattc agcgtgtcac
    12601 ttgccatatg catttcgaag gacggcgagt ggcgagtaat ttgcgaaacc acccgataac
    12661 ggagcggcgg tgttctcacg ataccgacac tctcaccaga tagcagcgca gcaattgcgc
    12721 tggcagttac tgcaaactgg cgaagatcgt tttgtccaaa gggcaacgct ttgccgagtt
    12781 ccagcgtaca ggccagcgcg ccaaaatgtc tggcgctgaa atgggtaaac gtaccaccag
    12841 gctcctgatg gaacaccagt gcctccagac ccgccgcacc cagccacgtc aggaatttct
    12901 cgtcccaggg tatgtcgcgt tgcggtaata caccgaactg cggatgcaag gagccacgaa
    12961 ttgcggtatg tagatcaagg tgccagcgca cagattcttt accctggtca taaaaatctt
    13021 ccaggcactg ttccagttcg cgcgcccgac aggtttcccc gctttcagca aacagctgcc
    13081 aacgaccgcc aaacattcgg ttcatatcgc tatggcaata acgtttccct tgcttcagcg
    13141 caggaggatt cccgaggatt accagcaacc gccaacgtaa cgggatctcg ccgtgagata
    13201 tcgcgccaag caacgcgtcc agcatctcca caggtgccgt ctcattaccg tgtattcccg
    13261 ctgaaatcac cagtgcgcct tgcggtggcg ttaatggcgt cagttccagc acaccatcgc
    13321 ccagccagcg ccagcgaacg ccgttgattt ctcgctcggt gataaccggt tttttacccg
    13381 ttaaggtcag agcaagaaaa ttatccattg ccccctccct cgcgctgaaa cggataaacc
    13441 gaaccgagat tcattaattg gctcaataca tccagtgctt cccgcccttc gcgcagcaat
    13501 tgcgggtcgg ccagatcggc agcagtaaga cgatcgcggt agtaacgatc cacccagtca
    13561 ttgagcgcat taaacaaggt atcgttcatc atcactgccg gattcaccgc ccggcgttct
    13621 tcttctgtca ataccacccg caaccgcagg cacgccgggc cgccgccatt cgccatgctt
    13681 tcacgtaaat caaagacttt tagttcgctg atcgggttgt cagcggcaag gagttcattg
    13741 agataacgcc agactccagc gtgttcccga cactcctgcg gcagcaccag cagcatggaa
    13801 ccgtcatcgc ggctaagtag ctggctatta aacagatacg tagacaccgc atcagacacg
    13861 gagacctgag tcgccggaac ttctatcgcc ataaaaccat tgacccgcgc acgcaggttt
    13921 gccagtaact gtgactggcg agcgaacgcc tgttggtggc aaaacagcac ctggcggtta
    13981 ctcacggcaa tcacgtcatt atgaaaaacg ccttggtcga taacgtccgg gttttgctgg
    14041 gcgaaaatca cctgttgggg attcacctga ttcagccttg tcaccgcctc gctggcttcg
    14101 cgagtctggc gcgccggata acgggaaggc cgggtatcat tgccctcttc tcgcccgtag
    14161 acaaaaagtt gcatacccgg ttcaccgtaa tgaccgccga gacgattgtg gtttgccgcc
    14221 ccctcatcac cgagcaaggc tacctgcggc aacgccgaat ggacactaaa tttctcttcg
    14281 tcgttaaaaa tcgcttttaa cagcgattca gtgacgggcg cttccagcga acggtgaaat
    14341 ttattgttca ggttagcaac ggtgagatgc actttgccat ccagcgtatc ggcagatggc
    14401 gcgatcgttg ccgcattggc tacccacatt ggcgaagcgg agctgacgct ggaaagccag
    14461 tgcggtgcct ggcgtgcaac tttttccagt acctgctcat cgctaccact gaatcccaac
    14521 tgacgcagca ccggaataaa cggacgctcg tgcggcggga tcacggcctg ggggaatccc
    14581 gcatcggcaa gggttttcat tttcagtaag ccctgtttcg ccgccagtcg cgggttagag
    14641 acctgaaaac ggtgacgggt agaggcttta ttaccaaacg acaagcccgc gtaatgatgc
    14701 gtcagcccta ccagcccgtc gaaattaact tcccaggcgt tcatcgcacc acctcatcgg
    14761 aaaaatccag cccggggtta agcgttgcgg gcaaagttaa cgagtccgac tccaggctcg
    14821 ccatcggcca ggcgcagtaa tctgcggcat accaggcgct ggggcgatgg ttaccggaag
    14881 cgccaatgcc gccgaatggc gcggtactgg cagcgccggt aagcggtttg ttccagttaa
    14941 caatccccgc ccgcgcctcc agcaacagtt gttcgaattt ttcccgctcg ggggaaacca
    15001 gcccacaaga gaggccaaag cgagtgttat tcgccatcct gatcgcctca tcgaaggtgt
    15061 cgtaacgcca gacgcgcaat agcggcccga acacttcttc atccggcacg cctgcaacac
    15121 ccatcatttc aataatcccc ggcgtcaaca gcgatgttcc cgcttgcagc aaacgcggcg
    15181 caagtagggt tcgtccgccc atcgcttcaa gttcctgcca ggcaatcacc acctgctgcg
    15241 cggcctgttc agaaatcagc ccgccaataa acggctgcgg ttcgtcatcc cagttgcccg
    15301 gcgttaatcg ctggctgacg gcaaccaggc gagcaagaaa tgcatcgccc tgcgctccac
    15361 ttttcagcaa taaacggcga gcgcaggtgc agcgttgacc agctgtgaca aacgccgact
    15421 gaatggtcag atggacagcg gcatcgatat ccgccacctc atcgataatt agcgggttat
    15481 taccgcccat ctcaagggcg agaattttct ccggctgacc ggagagctgg cgatgcagct
    15541 ggtagccggt attggcgctg ccggtaaaca gcaagccgtc aagatcctcc agcgcactca
    15601 gcgcctgacc cgtttcacgc ccgccctgca ccaggttcag cacgcccggc ggcaagccag
    15661 cctgctgcca taaacgcatt accgcttcgc cactccacgg tgtcagttcg ctgggtttaa
    15721 agataatggt gttacctgcc agcaatgccg gaacgatatg tccgttcggc aaatgaccag
    15781 ggaaattata cggcccaaac accgccaaca cgccgtgcgg gcgatgtcgc aggctcgccg
    15841 cgccgtccgg catttcacta cgctgctcgc cggtacgaac gtgatatgcc ttaattgaaa
    15901 tcgcgatttt attgatcatc gccgtcactt cggttgccgc ttcccagcgc ggcttacccg
    15961 tttctctggc aataatcgcg gttaattcgg ctttattgct ttccagcaat ccggcaaagc
    16021 gttcgacaac ggcctgacgt tcagcaaatg agagccgcgc ccagcgcgga aacgccgcac
    16081 gggctgcgcg acaagcctgc ccgacctgag cggcatcggc atcattgcct tgccataaca
    16141 cctcgcccga taccggatta cgcttcacac gcaatgcgcc ctggcccgtt atccagtcac
    16201 cgttaatcca taaagtcatg ctgttttctc ctctgcgcac aggcgcacca gacgaacgcg
    16261 atcccctgcg tggcatttga gggcatccag ttgtgcggcg gttaaaatca aacgctcggt
    16321 tgccggatcg gtacgcacca gcaccacgcg gaaatggtga taattttcat tggcgaccag
    16381 gcaggccggg aaatcgccct gcgcaggctg tccttctgcc acttccacca gccgactttt
    16441 acggatggcg cgcacgcggt cgatatcaca ctcaagcgtc ggcccgccgt caaaaatgtc
    16501 gatatagttg cggtaacgaa aaccttcttt ctccagcacc gcgcgggcag gcgcggtttg
    16561 cggatgtacc tgaccgatga cgtcctgggc ttcctgggat aaaaagtggg tatagatcgg
    16621 atgtttcggc atcagttctg caataaatgc cttttgcccg gtgccgcaga gaaaatcggc
    16681 gcggctaaaa tccatcgaaa agaagcgctt gccgaggctt tgccagaacg gtgaatagcc
    16741 gtgttcatca atcaccccgc gcatttcggc gaccactttg tcattaaact tgtcgcgaaa
    16801 agccgccata aacataaagc gcgatttcga cagcaaatag ccgttgccct ctttgcgcca
    16861 gtccgggtcg agaaacagcg tacacagctc gctgctgccg gtgtgatcgt tactgagaaa
    16921 cagcgtcggc agcgcgttat agacattcag ctctttcgaa gcgtgaacca gcgtgccgac
    16981 gcgatagttg taccagggat cgttcagccc aactgccacc tcaatggcac aaatccccgc
    17041 cacggtgcct gtctcgctat cttccagtac aaacacataa ccctgctcac ttttgggcag
    17101 ttcaccttgc caggttttga ttgccctttc gatacgcgcc gaaagcgtgg cttcattggc
    17161 gggaagcgac gtcaggccgc cgcccgtttt gctggcaagc tgcatcagcg ccgagacatc
    17221 tgagcgctca acgggacgga tgaccatcat gatgaacctc ggctaacaaa gtgttcgcaa
    17281 gcaactgcaa agcgatccag tccggtcgtc acctcttctt cgctgacatt gagcgcaggc
    17341 gcaaaacgca ccacgttgcc acccgcaatc agtaccatca cgcctgcttt cgccgcttcc
    17401 tgagagatct gtttcgcttg cccggcgtaa tcggcattca gtacacagcc aatcagcaaa
    17461 cctaagccgc gaacttcact gaacaaacca tagcggtgat taataatatt aagacgctca
    17521 acaaaccagt cgtgacgttg cttaaccccg ttaagcatct ctggcatatt gatgagatcc
    17581 agcactttgc ctgccaccgc cgaggccagc ggattgccgc cataggtggt gccatgagtg
    17641 ccaacggtca tcacgctggc gcactcttcg gttgccaaca acgcaccgac cgggaaaccg
    17701 ccgcccagcg ctttggcggt agttaacaga tcaggcgtca cgccgtagtg catataggca
    17761 tacagttccc cggtgcgccc gacgccggtt tgtacttcat caaaaatcaa cagcgcattg
    17821 tggcggtcac acaattcacg caggccttgt aaaaatgcgt tgctggctgg caccacaccg
    17881 ccttccccct ggatgggttc gacaatcacc gcacaggtag agtcgtcaat cagcgcgctg
    17941 gcagagttaa tatcgttata tgcagcatga cgaatatccg ccggcagtgg cgcaaaatcc
    18001 tgtgaatagg ctggctgccc acccgcacta acagtaaaca gcgtgcgacc atgaaacgca
    18061 tttttaaacg ccacgatacc gctcttatgg ctgccgtagc ggtcgtgagc gaatttacgc
    18121 gccagtttta gcgccgcttc gttggcttcc gcgccagagt tacaaaagaa gacgcgatcg
    18181 gcaaacgtgg cgtcgatcaa ttttttcgcc agtcgcagta ccggttcgtt ggtgtaaccg
    18241 ttgccggtat gccagaactt actcgcctgt tcgttcagcg cctcgcgcag ttccgggtgc
    18301 gcatggccca gcgcgttcac cgcaatgcca cccgcgaagt cgatatactc tttcccctgc
    18361 tgatcccaca agcgcgaacc ttcgccacgt accggtataa acggtgccgg agcgtaaaca
    18421 ggtataatcc attcatcaaa gttttcacgc gtaattggct gagacatagc gacctctaca
    18481 gtaaataatt cgttatttat atgttaataa taagtaatgt ttgcgctgta aatgtagatt
    18541 gcagggttcg tgccagccag tgataaaagt gcataaaccg cggaggctaa ctggaaatca
    18601 aggagttata accaaaccat atgcatttaa agtgcatatt aagtgaatac gtttgcgatg
    18661 tgggtgaata aaaagaataa aaaacgcaat gttatgcaga agtaaaatat aattctggaa
    18721 ttgtgatcat tgacgaaatt tactggaaat tactgcgcca ttctgacgca gcgcgcacca
    18781 aaagcgggca ttttttgcgc catcgttgac atcattaaca accatcgatc aaatcactta
    18841 acaacaggcg gtaagcaaag cgaaattctg ctaccatcca cgcactcttt atctgaataa
    18901 atggcagcga ctatgaaatt tgtctctttt aatatcaacg gcctgcgcgc cagacctcac
    18961 cagcttgaag ccatcgtcga aaagcaccaa ccggatgtga ttggcctgca ggagacaaaa
    19021 gttcatgacg atatgtttcc gcttgaagag gtggcgaagc tcggctacaa cgtgttttat
    19081 cacgggcaga aaggccatta tggcgtggcg ctgctgacca aagagacgcc gattgccgtc
    19141 cgtcgcggct ttcccggtga cgacgaagag gcgcaacggc ggattattat ggcggaaatc
    19201 ccctcgccgc tgggcaatgt caccgtgatc aacggttact tcccgcaggg tgaaagccgc
    19261 gaccatccga taaaattccc ggcaaaagcg cagttttatc agaatctgca aaactacctg
    19321 gaaaccgaac tcaaacgtga taatccggta ctgattatgg gcgatatgaa tatcagccct
    19381 acagatctgg atatcggcat tggcgaagaa aaccgtaagc gctggctgcg taccggtaaa
    19441 tgctctttcc tgccggaaga gcgcgaatgg atggacaggc tgatgagctg ggggttggtc
    19501 gataccttcc gccatgctaa tccgcaaaca gcagatcgtt tctcatggtt tgattaccgc
    19561 tcaaaaggtt ttgacgataa ccgtggtctg cgcatcgacc tgctgctcgc cagccaaccg
    19621 ctggcagaat gttgcgtaga aaccggcatc gactatgaaa ttcgcagcat ggaaaaaccg
    19681 tccgatcacg cccccgtctg ggcgaccttc cgccgctaat ttagcaactc tcctggctca
    19741 aactgggtca ggagaattaa ccttgagaaa aatcaacaaa ctgtcagtaa tgatttgttg
    19801 cctgccgtcc tttgttatac cgtcactgcg tttttagttg tctgaccact tctctattat
    19861 caagtttgat ataggaaact ccacgatgaa cgctgagcgt aaatttctgt ttgcctgtct
    19921 tatttttgcg ctggtcattt acgctatcca cgctttcggt ttattcgatc tgctcaccga
    19981 tttaccccac ttacagacac tcattcgcca gagcggattg ttcggttata gcctctatat
    20041 tctgttattc atcattgcca ccctctttct gttaccagga agcatactgg tgatcgcggg
    20101 aggaatagtt tttggcccgc tcttagggac actactctca ttaattgccg ctacgctggc
    20161 ctcctcgtgc tcattcctgc tggctcgctg gctgggacgt gatttactgc tgaaatacgt
    20221 tggccatagc cataccttcc aggccattga aaaaggcatt gcgcgtaacg gtattgattt
    20281 tcttattctg acccgcttaa tcccgttgtt tccttacaat attcaaaatt acgcttacgg
    20341 attaaccaga atcgccttct ggccttatac ccttgtttcg gcactcacga ccctgcccgg
    20401 tattgttatt tataccgtga tggcaagcga tctcgccaat gaaggcatta cgctgcgctt
    20461 tattttacaa ctctgtctgg cgggcctggc gctgtttatt ctcgtccagc tcgcaaaact
    20521 ctacgcccga cacaaacatg tggatctgtc tgcttcgcgc cgcaacccac ttactcaccc
    20581 caaaaatgaa ggatagaacg atgttgcaac attattcagt gtcatggaaa aaaggactga
    20641 ctgcactctg cttactggct gttgccgggc ttagcggctg cgatcaaaaa gagaatgcgg
    20701 cggcaaaagt ggaatacgac ggactttcga acagccaacc gctgcgcgtc gatgccaata
    20761 accatacggt aaccatgctg gtgcaaatta atggtcgttt cctcaccgac gacactcgtc
    20821 acggtattgt gtttaaagat ggctccaacg gacataaatc gctgtttatg ggttatgcga
    20881 ccccgaaagc attttatgaa gccctgaaag aggcaggtgg tacaccgggc gaaaacatga
    20941 cgatggataa taaagaaacg actcatgtca caggcagcaa actggatatt tcggtcaact
    21001 ggcaaggggc ggcaaaaacg tattccttcg atgaagtgat tgttgacagt aatggcaaga
    21061 aactggacat gcgctttggc ggtaatttaa cggcagcaga agagaagaaa acgggctgcc
    21121 tggtgtgtct ggatagctgc ccggtcggca tcgtcagcaa tgcaacatac acttatggtg
    21181 cggttgaaaa acgcggtgaa gttaaattca aaggcaatgc ctcggttctc ccggcggata
    21241 acacgttggc aacggttacc tttaaaatca ccgaataaag ccaggataaa ggatgatgaa
    21301 gatgcaatcg cgtaaaatct ggtactaccg tataaccctc atcatcctgt tgttcgctat
    21361 gctgctggca tgggcgctgc ttcccggcgt ccatgatttt atcaatcgca gcgttgcggc
    21421 gtttgccgcc gtggaccaac agggtataga acgctttatt cagtcttacg gcgcactggc
    21481 ggcagttgtc tcgttcttgt tgatgatttt acaggccatt gctgcaccgc tacctgcgtt
    21541 tttgatcacc tttgccaatg cgtcgctatt tggcgcgttc tggggcggct tgctgtcgtg
    21601 gaccagttcg atggccggcg cggcgctgtg cttttttatc gccagagtga tgggccgcga
    21661 agtggtggaa aaattaaccg gcaaaaccgt acttgacagt atggacggct ttttcactcg
    21721 ctacggcaaa cacactatcc tggtctgtcg gttattgcct tttgtccctt tcgatccaat
    21781 cagctatgct gccggtttga cttcaatccg ttttcgctca ttttttatcg ccaccgggct
    21841 tggtcagtta ccggcgacta ttgtttattc ctgggcgggc agcatgttaa caggcggtac
    21901 cttctggttt gtcaccggac tgtttattct gtttgccctg agcgtggtga tttttatggc
    21961 gaagaaaata tggcttgagc gccagaagag gaatgcctga tgggtttacc gccgcttagc
    22021 aaaattcctt tcattttacg tccgcaggcg tggctgcatc gtcgccatta cggcgaggtg
    22081 ctaagcccca ttcgctggtg ggggcggatc ccgtttatct tttatctggt gtcgatgttt
    22141 gttggctggc tggagcgcaa acgctcaccg ctcgatccgg tagtacgatc gcttgtcagc
    22201 gcgcgcattg cgcaaatgtg cctgtgtgag ttttgcgtgg atatcaccag tatgaaagtc
    22261 gccgagcgta ccggcagcag cgataaactg ctggcagtgg ctgactggcg gcaaaacccg
    22321 ctcttcagcg atgaagaacg gctggcgctg gagtacgccg aagccgcaag cgtcacaccg
    22381 ccaacggtcg atgatgccct gcgcacccga ctggctgccc attttgacgc tcaggcgctc
    22441 accgaactga cggcattgat cggcctgcaa aacctgtcgg ctcgttttaa ttctgccatg
    22501 gacattcccg ctcaagggct gtgccgtatt ccggaaaaac gttcttaagg agagatgatg
    22561 cgccattgtg ggtggttgct gggattgtta tcgctgtttt ctctggcaac acatgccagt
    22621 gactggcaag aaattaaaaa tgaggccaaa gggcaaaccg tctggtttaa cgcctggggc
    22681 ggcgataccg caattaaccg ctatctcgac tgggtgagcg gcgagatgaa aacccattac
    22741 gctataaacc tgaagattgt ccgtctggcg gatgccgcag acgcggtgaa gcgcattcag
    22801 accgaagccg cagccggacg taaaacgggc ggctcggtgg atctactctg ggtgaacggc
    22861 gaaaacttcc gcaccttaaa agaggccaat ttactgcaaa ccgactgggc agagactctg
    22921 cccaactggc gctatgtcga cacacagctg ccggtgcagg aagatttttc agtgcctaca
    22981 gaaggggctg aatcgccctg ggggggcgca caactgacat ttatcgcccg ccgcgatgtt
    23041 acgccacagc caccacaaac gccgcaagcc ttactggagt ttgctaaagc taatcccggc
    23101 acggttacct atccgcgccc accggacttt accggcacgg cgtttcttga acagttgctg
    23161 attatgctga cgcccgatcc cgccgcatta aaagaagcgc cgaacgatgc gactttcgcc
    23221 cgtgtcactg ctcccttgtg gcaatatctt gatgcgctgc atccgtattt gtggcgcgaa
    23281 ggaaaggatt tcccgccttc gcccgcgcgg atggatgctc tgctgaaagc cggaacattg
    23341 cgcctgtcgc tgacctttaa ccccgcgcat gcgcagcaaa aaatcgccag cggcgatttg
    23401 cctgcaagca gttacagttt tggctttcgc gaggggatga ttggcaacgt gcatttcgtc
    23461 accattcctg ccaacgcgaa tgccagtgct gcggcgaagg tagttgccaa tttcttgctc
    23521 tcacccgatg cgcaattgcg taaagcagat cccgctgtct ggggcgatcc ttctgttctc
    23581 gatccgcaaa aactgcctga tgggcagcgc gaaacattgc aatcaagaat gccgcaagat
    23641 ctgccgccgg tactggctga accgcacgca ggttgggtaa atgcgctgga acaagaatgg
    23701 ctacaccgtt acggtacgca ttaatctttt tgctgtgggc gatggtggcg gtgatttatg
    23761 caccgctgat cccggcagct ctcacgctga tatcacctgc tttgtcgttg acacactggc
    23821 aggcgttatt tgccgatccc cagttaccgc aggcattact ggcaacgctg gtgtcgacaa
    23881 ccatcgcggc ggtcggggcg ttgttgattg ccctgctggt gattgtggcg ctgtggtctg
    23941 ggccgaaatg gcagcgtatg tgcacccgtc tgccgtggct gctcgccatt ccccatgtag
    24001 cttttgccac cagcgccctt ctgctctttg ctgacggagg gctgctttat gactatttcc
    24061 cgtatttcac tccgccaatg gacaaattgg gtattgggct gggcttcacg ctggcggtga
    24121 aagaaagcgc atttctgctg tggatcttag cagcggtatt gagtgaaaaa cggcttttac
    24181 agcaagtcat cgtgctggat tcgctgggct acagccgctg gcaatgcctg aactggctgc
    24241 tgttgccctc cgtcgcgcct gcgctggcaa tggcgatgct ggcgattgtt gcctggtcgc
    24301 tgtcggtcgt ggatgtggcg attattcttg ggccaggtaa tccaccgacg ctggcagtaa
    24361 ttagctggca gtggttaact cagggcgacg ccgatcaaca aacgaaaggc gcactcgcca
    24421 gcctgctgct gatgttgtta ctcgccgcct acgttttgct gggctatctg ctatggtgta
    24481 cctggcggcg tactattccc cgcgtagatg gcgttcgtaa gcctgccacg cctttattgc
    24541 caggcattac gctggcgagt tttttaccct tgaccggtgt gctgtgtgtg gttctgctgg
    24601 cgatcctcgc ggatcagtcg acgatcaata gtgaagcgct catcaacagc ctgacaatgg
    24661 ggctggtggc gacattcatc gctttgctcc tgttactgct gtggctggaa tgggggccac
    24721 agcgtcgcca gttgtggcta tggttgccca ttttattacc ggcactgcag ctggtggcgg
    24781 gccagtacac gctggcgcta tggctgaaca tggatggaag ctggacggcg gtggtctggg
    24841 ggcatctgct gtgggtaatg ccgtggatgc tgtttatcct gcaacccgca tggcagcgca
    24901 ttgattcacg gttaattttg attgcacaaa cactgggctg gtcgcgggcc aaaatcttct
    24961 tttacgtgaa atgcccactc atgttgcgcc ctacgctgat tgccttcgcg gtgggatttt
    25021 cagtcagtat tgcgcagtat atgccgacgc tgtggctggg cgcggggcgt tttccgacgc
    25081 tcaccactga ggcggtggca ttaagcagcg gcggcagcaa cggtattctc gccgcccagg
    25141 ctttatggca actgctatta ccgcttacta tttttgccct gaccgcgtta gtcgcaaaat
    25201 gggtaggtta tgtcagacaa ggactccgct aatgctctac gtgaaaaatg tttcgctacg
    25261 tttaccagaa agccgcttgc tgacaaacgt taactttacg gtggataaag gtgacattgt
    25321 cacgttaatg ggtccgtctg gctgtggaaa atccactctg ttttcatgga tgattggtgc
    25381 actggctgga cagttttctt gtacaggtga gctatggctc aatgagcaac ggattgacat
    25441 gctacccacc gcaaagcgtc atattggcat tcttttccag gatgcactgt tatttgacca
    25501 gttcagcgtc gggcaaaatt tactgctggc gctaccggcg acacttaaag ggaatgcccg
    25561 acgtaatgcc gtgaatgatg cacttgagcg ttcaggcctt gacggagctt tccatcagga
    25621 tcctgccact ttgtctggcg gtcagcgagc gcgcgttgct ctgcttcgcg cccttctcgc
    25681 tcagccaaaa gcgttgctac tggatgagcc attcagccgt cttgatgtgg ctctgcgcga
    25741 taattttcgc cagtgggtgt tcagcgaagt tcgcgaactg gcgatccccg tcgttcaggt
    25801 aacgcacgat ctccaggatg ttcctgctga tagttctgtt ctggatatgg ctcagtggtc
    25861 agaaaattac aacaaactgc gataacgcaa agtttttccc aatgcgtcag ttcagaatgg
    25921 cgcactcaaa actacaatgt cgggattttc gatgaaacgt gtttctcaaa tgaccgcgct
    25981 ggcaatggct ttagggctgg cttgcgcttc ttcgtgggcc gctgaactgg cgaagcctct
    26041 tacacttgac cagcttcaac aacaaaatgg caaagcgata gatactcgcc ccagcgcgtt
    26101 ttataacggc tggccacaaa ccttaaatgg cccttctggt catgaacctg ccgccttaaa
    26161 cctctctgct agctggcttg acaaaatgag caccgaacag ctaaacgcgt ggatcaagca
    26221 acataacctg aaagccgatg ccccggtggc gctatacggt aatggcaaag atgtcgacgc
    26281 cgtcaaaacg cgactgcaaa aagcaggttt tacgcatatc tccattctga gtgacgcgct
    26341 aagcgaacct tcccgtctac aaaaactgcc gcactttgag cagctggttt atccgcaatg
    26401 gctgcacgac ctgcaacaag gtaaagaggt tacggcgaaa cctgccggag actggaaagt
    26461 cattgaagcg gcctggggcg ctcctaagct ttaccttatc agccatattc ctggcgctga
    26521 ctacatcgat accaacgaag tggaaagcga accgctgtgg aacaaagttt ctgatgaaca
    26581 actaaaagcg atgctggcaa aacacggcat tcgccatgac accacggtca ttctgtacgg
    26641 gcgtgacgta tatgctgcag cgcgtgtggc gcagattatg ctttatgctg gcgtgaaaga
    26701 tgtgcgcctg ctggatggcg gctggcaaac ctggtccgac gcgggactgc ctgttgagcg
    26761 cggaacgcca ccgaaagtga aagcggaacc ggatttcggc gtgaagatcc cggcgcaacc
    26821 gcagttgatg ctcgatatgg aacaggcccg tggactgctg catcgccagg atgcatcgct
    26881 ggtgagcatt cgttcgtggc cagaatttat cggtacgacc agcggttaca gctatattaa
    26941 accaaaaggt gaaatagccg gagcacgttg gggacacgct ggtagcgact cgacgcatat
    27001 ggaagatttc cataacccgg atggcactat gcgtagcgcc gatgatatta ccgctatgtg
    27061 gaaagcatgg aatatcaaac cagatcagca agtttcattc tactgcggca ccggctggcg
    27121 cgcgtccgaa acctttatgt acgcacgagc aatgggctgg aataacgtct ccgtttacga
    27181 cggcggctgg tacgaatgga gcagcgatcc aaaaaatccg gtagcaaccg gtgaacgcgg
    27241 cccggacagc agcaaataac attgcaatta ctgacgctgg agcaacttca gcgtcagata
    27301 cccactccac acccgcgtaa aggtcgtcat ccagcacaac gcaccgaaga tccacgcaaa
    27361 ccacgcaaac catgcaggga ataaacatcc cagcacaaac agtaagatcg tttctgtgcc
    27421 ttcggttaat ccccccagat aataaaacga tttatgcgca taaccgggat tatcaatctg
    27481 atgtttcgcc gccagcgcag caaaagcgag aaaactgctg cccgtaccga taaacgcaaa
    27541 cagcagccag ccgcctgcca gcgcattttg ctccggtgca gcaagaataa aaccaaacgg
    27601 taccagcgcg taaaagagaa aatcgagaga aatatcgaga aagccgcccg cgtcggtaag
    27661 ccctctgcgc cgcgccagtg cgccatccag accatcaagc agacgattca acaaaatgac
    27721 gactagcgcc gccagatacc agcccagagc cagaaacggc agagccagca cgccgatggc
    27781 aaatcccact aatgttaaac catctggcgt aatgcccggt ttatcaagaa cccgcacgca
    27841 ctgatgcaac aacggtttaa tccggggatg aagatggcgg tctagcacag gcactcctta
    27901 aatataaagc ctttctgatt gagcaacagt gcggatatta tggcattttt cgcttatctg
    27961 cccgtgtgta atttatgaaa atgattgaag ttgttgccgc catcattgaa cgtgatggca
    28021 aaattttact cgcgcaacgc cccgcccaga gcgatcaggc gggattatgg gagtttgccg
    28081 gtggtaaagt cgagctggat gaaagccagc agcaggcgct ggtgcgtgag ttacgcgaag
    28141 aactgggcat cgaagcaact gtgggtgagt atgttgccag ccatcagcga gaagtttcgg
    28201 gccggattat ccatcttcat gcctggcacg tacccgactt ccacgggacg ttacaggcac
    28261 atgaacatca ggcgctggtc tggtgctcac ctgaagaggc gctgcaatat ccgctggctc
    28321 ctgctgacat tccattatta gaggcgttta tggctttacg cgccgccaga ccagcggatt
    28381 agtgctaagg gttttgtcat cccgctggca ttgcagcagt attccctcgg ctttaattac
    28441 cgctccttca gaataatttt tatcctgata aacgcagcac tgagtacagg gctgcgctga
    28501 ctgcccgcct gaactgaaca cttctggcgg tacgttaacc tccacgtccg gacgataatg
    28561 cgggttagcc agtgcaatta atggaaatgc taatactacg gcgaacaatg ctcgactcac
    28621 agggaactcc ttaacgttat tgtctctgct actgataacg gtagccgggt ggcaaaactt
    28681 tagcgtctga gttatcgcat ttggttatga gattactctc gttattaatt tgctttcctg
    28741 ggtcattttt ttcttgctta ccgtcacatt cttgatggta tagtcgaaaa ctgcaaaagc
    28801 acatcacata aacaacataa gcacaatcgt attaatatat aagggtttta tatctatgga
    28861 tcagacatat tctctggagt cattcctcaa ccatgtccaa aagcgcgacc cgaatcaaac
    28921 cgagttcgcg caagccgttc gtgaagtaat gaccacactc tggccttttc ttgaacaaaa
    28981 tccaaaatat cgccagatgt cattactgga gcgtctggtt gaaccggagc gcgtgatcca
    29041 gtttcgcgtg gtatgggttg atgatcgcaa tcaggtacag gtcaaccgtg catggcgtgt
    29101 gcagttcagc tctgccatcg gcccgtacaa aggcggtatg cgcttccatc cgtcagttaa
    29161 cctctccatt cttaaattcc tcggcttcga acaaaccttc aaaaatgccc tgactactct
    29221 gccgatgggc ggtggtaaag gcggcagcga tttcgatccg aaaggaaaaa gcgaaggtga
    29281 agtgatgcgt ttttgccagg cgctgatgac tgaactgtat cgccacctgg gcgcggatac
    29341 cgacgttccg gcaggtgata tcggggttgg tggtcgtgaa gtcggcttta tggcggggat
    29401 gatgaaaaag ctctccaaca ataccgcctg cgtcttcacc ggtaagggcc tttcatttgg
    29461 cggcagtctt attcgcccgg aagctaccgg ctacggtctg gtttatttca cagaagcaat
    29521 gctaaaacgc cacggtatgg gttttgaagg gatgcgcgtt tccgtttctg gctccggcaa
    29581 cgtcgcccag tacgctatcg aaaaagcgat ggaatttggt gctcgggtga tcactgcgtc
    29641 agactccagc ggcactgtag ttgatgaaag cggattcacg aaagagaaac tggcacgtct
    29701 tatcgaaatc aaagccagcc gcgatggtcg agtggcagat tacgccaaag aatttggtct
    29761 ggtctatctc gaaggccaac agccgtggtc tgtaccggtt gatatcgccc tgccttgcgc
    29821 tacccagaac gaactggatg ttgacgccgc gcatcagctt atcgccaatg gcgttaaagc
    29881 cgtcgccgaa ggggcaaata tgccgaccac catcgaagcg actgaactgt tccagcaggc
    29941 aggcgtactg tttgcaccgg gtaaagcggc taatgctggc ggtgtagcaa cgtcaggcct
    30001 tgaaatggcg caaaacgctg cgcgcctggg ctggaaagcc gagaaagttg acgcacgttt
    30061 gcatcacatc atgctggata tccaccatgc ctgtgttgag catggtggtg aaggtgagca
    30121 aaccaactac gtgcagggcg cgaacattgc cggttttgtg aaggttgccg atgcgatgct
    30181 ggcgcagggt gtgatttaag ttgtaaatgc ctgatggcgc tacgcttatc aggcctacaa
    30241 atgggcacaa ttcattgcag ttacgctcta atgtaggccg ggcaagcgca gcgcccccgg
    30301 caaaatttca ggcgtttatg agtatttaac ggatgatgct ccccagggaa catttcttat
    30361 gggccaacgg catttcttac tgtagtgctc ccaaaactgc ttgtcgtaac gataacacgc
    30421 ttcaagttca gcatccgtta actttctgcg atagcagcag atatgccagt aaagaaatcc
    30481 cattagacta tttttttgat aatcttcttc gctttcgaac aactcgtgcg cctttcgaga
    30541 agcaagcatt atataatgcc aggccagttc ttcttcaatt gtcccgtttt gaaaagctgt
    30601 gcttgatatc gagatcatcc atgataattc cgccgcccat attagcttcg ccgaggattt
    30661 actggagcta tgattagcgc aatcagagat atagtctgag ggaaaaacag caaatttatt
    30721 caacaaggcg ataacctgct ctggggcttc ctccatgttt gctttaaagg tattggctcc
    30781 atgatcgcca gaaagaaaat gctccattaa ggcacaataa ctttcgctat cttcgatacc
    30841 ccattgatcc tctaaagact cgcgtctttt acttatgata tcgatcgagt caaaaggaag
    30901 cacatgatat tggaaggtat ctttgccagg ttcaggcttt cgcggccaga actccagcgt
    30961 ttcagaccat tgcttatgat agaatcgata aggtgcgatc aattgtagcg cctgtaactt
    31021 ctcgatactg agcggctcaa tacctttagc ctgataataa tgcagctgtt ctttttttgc
    31081 tttaaaaccg gcccgaacaa taagccccat cataattaat agataaagaa aagagcatcc
    31141 cgcggtaatc aggcctcttt cattcaaacc gttggatgtt atcgctgcga atacaaacat
    31201 tacagcgaca gcacatgtta aatacccccc ccacttacaa agcagcatgg ccttattttc
    31261 tttaatcatc cgttcaaaat tactattaca tatttcccag ccattaaaag aatacttctc
    31321 gctcccagga tggttttgta ataaaacttt tttcatcaca ttccctgtta ttacatactt
    31381 tagaggcgat aattttatcc atgcaaaaaa atatccaacg aattttcttg atccgctgga
    31441 tatttcagaa tatgactcga atagcacgaa agattcgctc gcttacgcta tcgccccgct
    31501 tccggcttca tctgctggcg gacttttttt cgcactacgt ttacgcggtg cagccttttt
    31561 cttatcagca ctgccaccac tgcccggagc cacaatgccg cgaaactgcc gcaccggagt
    31621 acgtttggct tgatcaataa gctgatatag cgtccccacc agcggctgca taaagtcctg
    31681 atagcgacac tgcttttcgc tgatttgcgt cagcaccgat tcccagtgcg cggtcatgtc
    31741 cggtcgcgta gccatttctg gcagcgaatg gaataacgct tttccggcgt cggtggagtg
    31801 gatatagcgc ccttttttgg tgaggaaacc acgcttgaac aacagttcaa taatcccggc
    31861 acgcgttgct tctgtcccca gaccatcggt cgcacgaagg atctttttca gatctttgtc
    31921 ctgcacaaag cgcgcgatcc cggtcatcgc cgaaagcagt gttgcatcgg taaaatggcg
    31981 cggcggctgg gtctgccgct ctaccacttc acctttttca cacagcaact catcgccttt
    32041 cgccaccaca ggcagtggcg tgccgtcgtt ttcttcatcg cgctctttgc tgcctaacag
    32101 cgtgcgccag cctgcttcag caagaaaacg cgctttagcg acaaatttgc ctttggcaat
    32161 gtccagttcg ataacacact tgcggaacac cgcatccggg cagaactgca tcaagtactg
    32221 acgggcaatc aggttataga ccttcgcttc gttctccgtc aggttgatcg cagaactccg
    32281 tgcggtcgga atgatggcgt ggtgcgcatc gacctttttg tcatcccaac agcggttgcg
    32341 tatatctgga tctaccactg gctgcggcaa cagatctggt gcatgaacac tgatggcatt
    32401 catcaccgcg tggcgtccgg caaaatgttc ttctggcaaa tagcgacaat cggaacgcgg
    32461 ataagtgatt agcttgtgtg tttcgtacag cttctggcag atatcaagca cgttctgcgc
    32521 actcagacca aagcgttttg ccgcttcaat ctgcaacgcc gaaagcgaaa aaggcagcgg
    32581 cgcggattct gattcccgtt tatcgttata gctggtgaca atagccggtt gaccactaat
    32641 gcggttaacc acatgctctg ccagtggacg atgtaacaag cgcccttctt catcctggta
    32701 cggttcacac gcttcgctcg gttgccagat agcggtaaac cgctcttcgg caggtgtcac
    32761 gatatgcgct ttgacttcaa agaagtcttt cgccacgaag ttttcaatct cttcatcgcg
    32821 gcgcaccacc agcccgagca cgggtgtctg cacgcgtccc acggaaagta cgccctgata
    32881 accggcattg cgaccgagaa tggtatacgc acgggtcatg ttgatgccgt atagccagtc
    32941 ggcacgcgct cgcgccagcg cagaaacgca cagcggtaca aactcactgt tggaacgaag
    33001 acggtcgatc gcccgctcaa ccgcctgcgg gttcaggtcg tttatcaagc aacgctgcac
    33061 ctgctggcgc ttttccggtg ccagttgcag atagtccagc acttcatcca ccagcagttg
    33121 cccttcacga tccgggtccc cggcgtgaac gatttcgctg gcttcatgca ggaaccgttt
    33181 gatgacgtta agttgtttgg tcacggaggg tcggggctgt aattgccact tttccgggac
    33241 aatcggcaaa tccgcaagat tccagcgcgc atagcggctg tcgtaggcgt ctggctgcgc
    33301 ctgctcaagc aggtgaccga tacaccaggt caccacctga ccattaccgc actcgataaa
    33361 gccatcgcct ttccggtgcg gtttgggcag gacatcagca atggcgcgcg ccagactcgg
    33421 tttttcggca ataaacaacc gcattgaatt aacgaatctc aaccatggca cgaccgccgc
    33481 gcgctggcac cagttcgcca atcgccgtca gttcaatgcc aaactcggcg gctgtagctt
    33541 taacctcatt ttctgcttcc ggcatgaccg ccagcagcaa accgccagaa gtttgcggat
    33601 cgcacagcaa atcgcgcact tcacgcggca tttcacccat cagatgaccg tagctggcaa
    33661 agttacgttc ggtgccgcca ggcactgcac ccaacttaat gtactcttca acccccggca
    33721 atttcgggat cgcgtcatag tcaacgcgcg cctgcacacc tgcgccctga cacatttcgc
    33781 tcaaatggcc cagcaggcca aagcccgtta cgtcggtcat cgcttttacg ccttcaatgt
    33841 tggcaaagga cgcgcctgcg atgttcatcc ggcacatcac ttccgtcgcc agtccctgat
    33901 gttctggttt cagcagtgat tttttctcag ccgtggtaag aacgccgatc cccagcggtt
    33961 tcgtcaggaa cagtttacat ccggcttgtg cggtactgtt tttcttcacc cgctcggtcg
    34021 gtacgatccc cgtcaccgcc aaaccaaaaa tcggctccgg cgcatcgatg gagtgaccgc
    34081 cagccagcgc aatacccgcc tgacgacatg catagcgtcc accttcggtc acttcgcggg
    34141 caatttccgg ggaaagttta ttaatcggcc agccgaggat cgcaatcgcc ataatcggct
    34201 tgccccccat cgcgaagata tcgctgatgg cgttagtcgc cgcaatgcgg ccaaaatcga
    34261 aaggattatc aacgatcggc ataaagaagt cggtggtact gataacgctg gtgccattgc
    34321 ccagatcgta caccgccgca tcgtcgcggg tttcattacc cacaagcaaa ttcggatcaa
    34381 caaacttcgc ctgctcactg tgcaggatgg tttccaacac ttttggggaa attttacagc
    34441 cgcaaccagc tccgtggctg tattgggtca aacgaatcga gttctcgctc atggacatct
    34501 cctgtcaatg caatccgggt atggtaaccc tcattccatg aagtgataag tgagagtgtc
    34561 tgaattcctg cgcctttgct cacaatccag acagtttcgc gacaattatc agaaataagt
    34621 tacaaacggc gtcgggtccg ggacgtttat cgacgtagat gctttcagct gcggcgtgcc
    34681 gaggtagaga aaaccgacaa ttttatcctg ctcacggcaa ccgaatgctt cacgcactac
    34741 tggactttca gttaatgcgc cactgcgcca gatgccgcca aacccctggg caactgctgc
    34801 catttgcatc gccatgaccg cgcatccggc agacatttcc tgttcccatc gcgggacttt
    34861 atgattttct tcgcatttcg ccaccaccgt gatgatgagc ggtgcgcgga acggcgcatt
    34921 acgcgctttg tcgatagctt tgtcatcact accggcagca atcgccccct gttccagtac
    34981 ggcgctgaaa cgctcgcgcc cttccccttc aatcacaaaa aaatgccacg gttgcatgga
    35041 cttatggtcc ggcgcgcgca tacccgcacg caggatgttt tgcagttgtt cacccgttgg
    35101 tgcgggttca gccaggcggg aggcgctacg gcgattgatc aatagttcga gtgcatccat
    35161 ttgattaact cctgtcatga tatttattca caaaattaac acgagagtgg attttgttac
    35221 agcacagtcc gcaattcctg ctgacaagtg ccggttgggt cattacgata accacatcta
    35281 ttgcgcctgt gacaggtgtg accttaagtt gggagaatac atgcgaaccc tttggcgatt
    35341 tattgccgga ttttttaaat ggacgtggcg tctgctgaat ttcgtccgtg aaatggtact
    35401 taacctgttc tttatttttc tcgtactggt tggtgtgggg atctggatgc aggtcagtgg
    35461 tggtgattcg aaagaaacgg ccagtcgtgg cgcactgctg ctggacattt ctggtgtgat
    35521 cgtcgataaa ccagacagtt ctcagcggtt tagtaaatta agccgccagc tgcttggtgc
    35581 cagttccgat cgtctgcagg aaaactcact gtttgatatc gtcaacacta ttcgccaggc
    35641 gaaggacgac cgcaatatca ccggtattgt gatggatctg aaaaacttcg caggcggcga
    35701 ccagccgtct atgcagtaca tcggcaaagc cctgaaagag tttcgtgaca gcgggaaacc
    35761 ggtttatgcc gttggcgaga actacagcca ggggcaatat tatctcgcca gtttcgccaa
    35821 taaaatttgg ctgtcaccgc aaggcgtggt ggatttgcac ggttttgcta ccaacggtct
    35881 gtactacaaa tcgttgctgg ataagctgaa agtttccacc catgtgttcc gcgtgggtac
    35941 gtataaatct gccgttgaac cgtttattcg tgatgacatg tcaccggcag cccgcgaagc
    36001 tgacagccgc tggattggtg agctgtggca aaactatctg aatactgttg ccgctaaccg
    36061 gcagatccct gctcagcagg tattccctgg cgcgcaaggg ttgcttgagg gtttaaccaa
    36121 aaccggtggc gatactgcga aatatgcact ggaaaacaag ctggtcgatg cactggcatc
    36181 gagtgcggaa atcgaaaaag cactgaccaa agagtttggc tggagtaaga ctgataaaaa
    36241 ttatcgcgcc atcagttatt acgattacgc attgaaaacg ccggcagata ccggtgacag
    36301 catcggtgtc gtcttcgcta atggcgcaat tatggatggc gaggaaactc aggggaatgt
    36361 tggcggtgat accactgcgg cacaaatccg cgacgctcgc cttgacccga aagtgaaagc
    36421 gattgtcctg cgtgttaata gcccaggcgg cagcgttacc gcgtctgaag tgattcgcgc
    36481 tgaactggca gcagcccggg ctgcgggtaa gcctgtggtt gtatcaatgg gcggcatggc
    36541 ggcatctggt ggttactgga tttccacgcc agctaattac attgtggcta accccagcac
    36601 cctgaccggt tctatcggta tcttcggcgt gatcaccacc gtagaaaata gtctggattc
    36661 gattggtgtt catactgatg gtgtctcaac ttcaccactg gcagaggttt ctatcaccag
    36721 ggcactgccg ccggaagcgc agctgatgat gcagttaagc attgagaatg gttataaacg
    36781 ctttatcacg ctggttgctg atgcgcgtca ttcgacgccg gagcagattg ataaaatcgc
    36841 ccagggccac gtctggaccg gtcaggatgc aaaagctaac gggctggtcg atagtctcgg
    36901 ggatttcgat gatgcggtcg ccaaagcagc agagctggca aaagtgaaac agtggcatct
    36961 ggaatattac gttgatgaac cgaccttctt cgacaaagtg atggacaaca tgtctggttc
    37021 tgtccgggca atgttgccag atgcgttcca ggccatgtta cctgcaccgc tggcctcggt
    37081 agcctctacc gttaaaagtg aaagtgacaa gctggccgcg tttaacgacc cacaaaaccg
    37141 ttatgcgttt tgcctgacct gcgccaacgt gcgttaagtc ttgtactgag tggccgacag
    37201 atcgtcggcc atatttttaa tcctcttccc gctgtctcgc cccatatttc cttatccgca
    37261 tagtatctgg tgcgctcccc ctgcctcacg tatatacttt tgctctttcg atatcattca
    37321 tatcaatatc atgcaaaaga aatcaattta cgttgcctac acgggcggga ccatcgggat
    37381 gcagcgttcc gagcagggtt atataccggt gtcaggtcat ctacaacgcc aactggcgct
    37441 gatgccggaa ttccatcgcc cggagatgcc agatttcacc attcatgaat atacgccgct
    37501 gatggattct tcagatatga cgccggaaga ctggcagcat attgctgaag atattaaagc
    37561 gcactatgac gactatgatg gttttgtcat tctgcacggc accgacacga tggcgtatac
    37621 cgcctctgcg ctgtcgttca tgctcgagaa tctcggtaaa ccggtcattg tgacagggtc
    37681 acaaatcccg ctggctgagt tacgctctga cggacaaatt aatctgctga atgcgttgta
    37741 cgttgcggcg aattatccga tcaacgaagt aacactcttt tttaataacc gactgtatcg
    37801 cggcaaccgt actaccaaag cccatgccga tggttttgat gcgtttgcct ctccaaacct
    37861 tcctccgtta ctggaagcag gtatccatat tcgtcgtttg aatacgccac ccgccccgca
    37921 cggtgaaggg gaattaatcg ttcatccaat caccccacaa ccaattggcg tagtgacgat
    37981 ttatccaggg atttctgctg acgtcgtgcg caattttctg cgccaaccgg tgaaagcatt
    38041 gattctgcgc tcctatggcg tgggtaatgc gccacaaaac aaagccttcc tgcaggaatt
    38101 acaagaagcc agcgatcgcg gtattgtggt ggtcaacctg acacaatgta tgtccggtaa
    38161 agtgaatatg ggtggttatg ccaccggtaa cgccctcgcc catgctggcg ttattggcgg
    38221 tgcagatatg actgtagaag ccacgctaac caaactgcat tacctgctta gccaggaact
    38281 ggatactgaa accattcgca aggccatgag ccaaaacctg cgcggtgaac tgacgccgga
    38341 tgattaagga gactgcaatg ccccctcgcg ccctgttact ggtcgattta caaaatgatt
    38401 tttgtgctgg tggcgcgctc gccgtgccgg aaggtgacag tacggtggat gtcgctaacc
    38461 gcctgattga ctggtgccag tcgcgcggtg aagcggttat cgccagtcag gactggcacc
    38521 cggcgaatca cggcagtttt gccagccagc acggtgtaga gccttatacg ccaggccaac
    38581 tcgacggttt gccacaaacc ttctggccag atcactgtgt gcagaacagt gaaggcgcac
    38641 aattacatcc gttactgaac caaaaagcga tcgcagcggt gttccataaa ggcgaaaatc
    38701 ctttagttga cagttacagt gccttttttg ataacggccg tcggcagaaa acctctctcg
    38761 atgactggtt acgcgatcat gaaattgatg aattgatcgt tatgggcctg gctactgact
    38821 attgcgtgaa gtttaccgtg ctggacgcgt tacagttagg ttataaggta aacgtgatta
    38881 ccgatggttg tcgtggagtg aatatccagc cccaggacag tgcgcacgcg tttatggaga
    38941 tgtcagctgc tggggcaacg ctatatacgc tggcagactg ggaagagaca caggggtaat
    39001 attagttgaa ttcatctctg gcctacattt cctcaatgac attgtaggcc agataatact
    39061 tgtcagtgtt gcatttaacc tacttgcagc taaaacacga cgtccgaaca agtatatttg
    39121 ccctccctta ataacaggct taaatactag ataactcatt cacttaaaca tggcaatagg
    39181 ctttgctgag ataccaaaat cttcttttaa ctgctgtttg ctcttcatga ccatctgacc
    39241 atttgtgtca attgtcatat gctgtgcttc ggtattattg cgggcttgcc acagcatcac
    39301 cagttgcagg cagttctctt tttgttcctc ggtcaacgca acgccatcag gccatttccc
    39361 cagttcaacg gcggtcgaca aacgctggta tacctcaggc gtcatgctgt tgataatgtc
    39421 atcaagattc ataatttttc cgctcctgtg gaataagttg ctgaatcgtt ttttcaaccg
    39481 ttgatttctt cgccgttttc gccatcagtg aagcgtaaag aagcagagtt aacacaataa
    39541 cgttcgcccg ttggctgcgg cccgtcaggg aagacatggc ccagatgggc atcacagtta
    39601 ccgcaacgaa tttctatgcg ctgcattcca tgtgacaagt ctttgatata acgaatggat
    39661 tcttcactca ccggttcgta gaaactgggc cagccacagc cggaatcata cttggtttgg
    39721 gaatgaaaca gcggggcatc gcagatcaaa cagtgatata cgccgtcacg cttgttatgc
    39781 agtaaacgac ccgtaaatgg cggctctgtc ccatgattct gcgtcacgta aaactgcatc
    39841 tcggacaaat tttttttcaa ttcttctgcc gaaggtttat tagccatttg ctcacatctc
    39901 actttaatcg tgctcacatt acgtgactga ttctaacaaa acattaacac caactggcaa
    39961 aattttgtcc taaacttgat ctcgacgaaa tggctgcacc taaatcgtga tgaaaatcac
    40021 atttttatcg taattgccct ttaaaattcg gggcgccgac cccatgtggt ttcaagccca
    40081 aaggaagagt gaggcgagtc agtcacgtaa tgcttaagca caggattgat ttgtcgcaat
    40141 gattgacacg attccgcttg acgctgcgta aggtttttgt aattttacag gcaacctttt
    40201 attcactaac aaatagctgg tggaatatat gactatcaaa gtaggtatca acggttttgg
    40261 ccgtatcggt cgcattgttt tccgtgctgc tcagaaacgt tctgacatcg agatcgttgc
    40321 aatcaacgac ctgttagacg ctgattacat ggcatacatg ctgaaatatg actccactca
    40381 cggccgtttc gacggtaccg ttgaagtgaa agacggtcat ctgatcgtta acggtaaaaa
    40441 aatccgtgtt accgctgaac gtgatccggc taacctgaaa tgggacgaag ttggtgttga
    40501 cgttgtcgct gaagcaactg gtctgttcct gactgacgaa actgctcgta aacacatcac
    40561 cgctggtgcg aagaaagtgg ttatgactgg tccgtctaaa gacaacactc cgatgttcgt
    40621 taaaggcgct aacttcgaca aatatgctgg ccaggacatc gtttccaacg cttcctgcac
    40681 caccaactgc ctggctccgc tggctaaagt tatcaacgat aacttcggca tcatcgaagg
    40741 tctgatgacc accgttcacg ctactaccgc tactcagaaa accgttgatg gcccgtctca
    40801 caaagactgg cgcggtggcc gcggcgcttc ccagaacatc atcccgtcct ctaccggtgc
    40861 tgctaaagct gtaggtaaag tactgccaga actgaatggc aaactgactg gtatggcgtt
    40921 ccgcgttccg accccgaacg tatctgtagt tgacctgacc gttcgtctgg aaaaagctgc
    40981 aacttacgag cagatcaaag ctgccgttaa agctgctgct gaaggcgaaa tgaaaggcgt
    41041 tctgggctac accgaagatg acgtagtatc taccgatttc aacggcgaag tttgcacttc
    41101 cgtgttcgat gctaaagctg gtatcgctct gaacgacaac ttcgtgaaac tggtatcctg
    41161 gtacgacaac gaaaccggtt actccaacaa agttctggac ctgatcgctc acatctccaa
    41221 ataagttgag atgacactgt gatctaaaaa gagcgacttc ggtcgctctt ttttttacct
    41281 gataaaatga agttaaagga ctgcgtcatg attaagaaaa tttttgccct tccggtcatc
    41341 gaacaaatct cccctgtcct ctcccgtcgt aaactggatg aactggacct cattgtggtc
    41401 gatcatcccc aggtaaaagc ctcttttgca ttacagggcg cacaccttct ctcgtggaaa
    41461 cctgcgggtg aagaagaagt tctgtggttg agcaacaaca caccgttcaa aaatggcgtc
    41521 gctattcgcg gtggcgtacc ggtttgctgg ccgtggtttg gtccggcggc acaacaaggc
    41581 ctgcctgcgc acggttttgc ccgcaacctg ccgtggacgc tgaaatcaca tcatgaagat
    41641 gctgatggcg tagcgctgac ttttgaattg acgcaaagcg aagagacgaa aaaattctgg
    41701 ccgcacgact ttacgctgtt agcgcatttc cgcgtgggta aaacctgtga aatcgatctt
    41761 gaatcacatg gcgaatttga aaccacctct gccctgcata cctactttaa cgtgggtgat
    41821 atcgctaagg taagcgtcag tgggctgggc gatcgcttca ttgataaagt aaatgacgcg
    41881 aaagaagatg tactgaccga cggtattcag accttccctg accgtaccga tcgcgtgtat
    41941 ctgaatccgc aagattgcag cgtgattaat gatgaagcgc tgaatcgtat tatcgccgta
    42001 ggccaccagc atcatctgaa cgttgtaggc tggaacccgg gaccggcgct ttcagttagc
    42061 atgggcgata tgccggatga tggctacaag acctttgttt gtgtagaaac agcttacgcc
    42121 tcagaaatgc aaaaagtgac caaagaaaaa cctgcacatc tggcgcaatc cattcgcgtt
    42181 gccaaacgtt aatttgcgtc aatgttgtgt gccgggtgcg atgcatccgg cacgtatcaa
    42241 catcacacca tatccagcgc agtttttcct tttggtgccg gatacgcttt gtccagcatg
    42301 gctaattccg ctgacgaaag ttcgacctca agcacagccg cattctgttg gacatgggca
    42361 atcgtggccg cttttggaat cgccatcaca ccctgatggc tgattaccca cgccaacaat
    42421 acttgtgccg tgctgatatt gtgagcatgt gcaatttcgt tgactaccgc gttttttaac
    42481 agtccattgc gcaaccgccc ggcctgggct aacggactgt aagccatcac cggcatctgc
    42541 tgttgctggc accaggggag tagatcgtac tcaatccctc gtgaaccgag atggtaaagc
    42601 acctgattag tggcacactg atttcccccc ggcagctgcc agagttcctg catatcagca
    42661 taatcaaggt tcgaaacgcc ccagcggcgg atttttccct gggcgatcaa tttttccatc
    42721 gctgccacag tctcttcaaa agcgaaactg ccagaccagt gtaataagta gagatcgaga
    42781 taatcagtat tgagacggcg taaactggct tcgcatgcat ttatcgcttt ttgcccgcca
    42841 gcattccacg gatagacttt agagacgaga aagacgttat cgcgcagacc ggttaatgct
    42901 tccccaacca ccttttcggc accgccatcg gcatacattt cggcggtatc aatgagggtt
    42961 aaaccgagtt caatgcccgc gcgtagtgca gcaacttctg ttttacactt actggcatct
    43021 tcgcccatat accatgttcc ctgccctatg gctggcagtg agacatcgcc actaaattga
    43081 atcatttttt gttgcattgt ttcctcccag gtattgcacc accgtaatgc aaaacagggc
    43141 gtgacgccct gtttttatgc acaaaatgcc ctggaaagat gcattatcag aatttgtagg
    43201 tgatcccggt agaaatcagg ccagtccagg atttatccac catcgggctg tcagtaactt
    43261 catcagacag acgggtatag cgcgcggtac cgtaaacact ccagtcgccg aggaagttgt
    43321 agctggcgct cagctccagg taagggctcc agctgtcgtt cgggttatag ccacgcagac
    43381 cgctgcgagc ggactctttg cgcgatacgc cataatagta ttcgttctgg ttttcgctgt
    43441 tccactgcac accgataccc ggggtcacgg tcaggccgcc gttggtgtaa cgatacaacc
    43501 aggccatatc ccagacaatg ccgttgctgt tatccagggt atcgccagcc agggtggtac
    43561 gcaggtaacc gtactgggta aagtgagcat aagacagacc agccatcatg gtgctcttac
    43621 ggtcatccag gtgacgcatt tggtgatcgc cactgtcttt cgctttgaag taaagcggcg
    43681 accaataagc ggtaattgaa agtttgtccg ttgcgtcatt ccacaggtag taaccaccac
    43741 ctaagccacg gaaccagaag ttatcgcctt catagttgat taccggtact gggtaaacat
    43801 cggtatcgta atctttatat gggtgttcaa cgacacctac gcctgcgccc agggaaaatt
    43861 taccttcagc gtgcgctacg cctgcagacg ttgcgataag cactccaagt gccagaagtt
    43921 tgagtttggt cacaattaat cattccttaa acaaatgttt agcgggcgac aaagtttacc
    43981 cgtcaataca tcgaaaccca acctttttac gttttcattt tttaaagtaa ctgtttaatt
    44041 ttcctgacgc ggatgacacc gcgcttacag ccaaatgaat ttagcgttac tggcgagcct
    44101 tgtctttaca ttaattatgc aaaatttatg gatgagttgt tgatatgcca ttgaaattaa
    44161 gaaagccgtg caggcaagtt ttccatttgc catctacgct taattttgaa ggtgtatcac
    44221 cgggcacgtt gttctcatcg tcgataaaat ggcatgagag ttgctgtgtt ttagcaagag
    44281 acgtcgttca gtttacctct tccgggagcc tctactattc atatgaacgg ctcttaacct
    44341 gtgctaaaaa acgaaaggac ggcataccat gaatatattc gatcactatc gccagcgata
    44401 tgaagctgcc aaggacgaag agttcacact gcaggagttt cttaccactt gtcggcaaga
    44461 tcgcagtgct tatgccaacg cggctgagcg gctattgatg gctatcggtg agcctgtcat
    44521 ggtcgataca gcccaggaac ccagactttc tcgactcttt tctaaccggg tcattgcacg
    44581 ttatccggcg tttgaagagt tttacggcat ggaagacgcg attgaacaga ttgtctctta
    44641 tctgaaacac gcggctcagg ggctggaaga gaagaaacaa atcctgtatc tgctggggcc
    44701 tgtgggtggg ggtaaatcat cgcttgctga gcgactgaaa tcattaatgc agctcgtacc
    44761 gatttatgta ttgagcgcca acggtgagcg tagcccggtc aacgatcatc cgttctgtct
    44821 tttcaatccg caggaagatg cgcagattct ggaaaaagag tatggcattc ctcgccgtta
    44881 tctcggcacc atcatgtcgc cgtgggcggc aaaacgcctg catgaatttg gtggcgatat
    44941 cactaagttc cgggtagtga aggtctggcc gtcaattctg caacaaattg ctatcgccaa
    45001 aacggaaccc ggtgatgaga acaaccagga catctccgcg ctggttggga aagtcgatat
    45061 tcgtaaactc gaacactacg cgcagaatga cccggacgcc tacggctatt ccggtgcgct
    45121 atgccgcgcc aaccaaggga tcatggaatt tgtcgagatg tttaaagcac cgataaaagt
    45181 gctgcatccg ctgttaaccg ccactcagga aggtaactac aacgggacgg aaggtatctc
    45241 cgccctgccg ttcaacggga ttattctcgc ccactcgaac gaatccgaat gggtcacttt
    45301 ccgtaataac aaaaacaacg aagccttcct tgaccgtgtt tacatcgtga aggtgccata
    45361 ttgcttgcgc atttccgaag agatcaaaat ctacgagaaa ttgcttaatc acagtgaatt
    45421 gactcacgcc ccatgcgccc ctggcacgct cgaaacactg tcacgttttt ccattctttc
    45481 acgcctgaaa gagccagaaa actccagcat ttattcaaag atgcgggttt atgatggcga
    45541 aagtctgaaa gacaccgatc ccaaagccaa gtcgtatcag gaatatcgtg actacgccgg
    45601 tgtcgatgaa gggatgaacg gtctgtcgac gcgttttgcg tttaagatcc tctcccgcgt
    45661 gttcaacttc gatcatgtag aagtggcggc aaacccggtc catctgttct acgtcctgga
    45721 acagcagatc gagcgcgagc agttcccaca agagcaggca gaacgctatc tggagttcct
    45781 gaaaggttat ctgatcccga aatatgccga gtttatcggc aaagagatcc agacggccta
    45841 ccttgaatcc tattccgaat atgggcaaaa cattttcgac cgttatgtta cctacgcgga
    45901 tttctggatt caggatcagg agtatcgcga tccggatacc gggcagctgt ttgaccgcga
    45961 gtctcttaac gccgagctgg agaaaatcga gaaaccggcg gggatcagta atccaaaaga
    46021 tttccgcaac gagattgtta acttcgtact gcgcgccaga gcgaataaca gcggacgcaa
    46081 tccgaactgg accagctatg aaaaactgcg cacggttatc gagaagaaaa tgttctccaa
    46141 taccgaggag ctgttgccgg ttatctcgtt taacgccaaa acgtcaaccg acgagcagaa
    46201 gaaacacgac gactttgtcg accgtatgat ggaaaaaggc tacacccgta aacaggtgcg
    46261 tttactgtgc gaatggtatt tgcgcgtacg taaatcgtct taacaaccct ggcccggtac
    46321 acatgctacc gggcctacaa cgacagcgaa ccgtgggcca gagaaatggc aacacaggcg
    46381 tagcatacag ttggcaaatg tagtacgggg ggcatatgac ctggtttatt gaccggcgtc
    46441 tgaacggcaa aaacaaaagc atggtgaatc gccagcgttt tttacgccgt tataaagcgc
    46501 aaattaaaca gtcgatctcc gaggccatta ataagcgttc ggtgactgac gtcgacagcg
    46561 gcgaatccgt atccattccc acggaagata ttagcgaacc gatgtttcat caggggcgtg
    46621 gcggcctgcg ccaccgcgtg catccgggca atgaccattt cgttcagaac gaccgaattg
    46681 aacgtcccca gggtggcggc ggaggttccg gcagtggtca gggccaggcc agccaggatg
    46741 gtgaaggtca ggatgaattt gtctttcaga tttcgaaaga tgagtatctt gatctgctct
    46801 ttgaagattt ggccctaccg aatctgaaac aaaaccaaca acgccagctg accgaatata
    46861 aaacgcatcg ggcgggttat actgctaacg gcgttccggc caatatcagc gttgtgcgtt
    46921 ccttgcagaa ctcattggcg cgacgcacag ccatgacggc aggcaagcgg cgggaacttc
    46981 atgcactgga agagaatttg gccatcatca gcaacagtga acctgcgcag ctgctggaag
    47041 aggaacgtct gcgcaaagaa attgcagaat tacgtgccaa aattgaacgc gtccctttta
    47101 ttgacacctt cgatttacgt tacaagaact acgagaagcg gcccgatccc tccagccagg
    47161 cagtgatgtt ttgcctgatg gacgtttccg gttcaatgga tcaatccact aaagatatgg
    47221 ctaagcgttt ttatattcta ctgtatctgt tcctcagcag aacgtataag aacgtggaag
    47281 tcgtatacat ccgccatcat acccaggcga aagaagtcga tgaacatgag tttttctact
    47341 cgcaggaaac tggcggcacc attgtttcca gcgccctgaa actgatggat gaggtagtga
    47401 aagagcgtta taacccggca cagtggaata tttacgctgc acaagcatcg gacggcgata
    47461 actgggccga tgactctccg ctttgccatg aaatcctggc gaaaaaatta ttacctgttg
    47521 ttcgttatta cagctatatc gaaattactc gtcgtgcaca tcagacattg tggcgagaat
    47581 atgagcatct gcaatctact ttcgacaact ttgcgatgca gcacatccgc gaccaggatg
    47641 atatttatcc ggtgttccgt gaactgtttc ataaacaaaa tgcaacagct aaagactaaa
    47701 actatcagcc gggtcattat cgcctggctg atttttagct tactgt
//
LOCUS       DAPGOF010000013        46069 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.013, whole genome shotgun sequence.
ACCESSION   DAPGOF010000013 DAPGOF010000000
VERSION     DAPGOF010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46069
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.013"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            88..1068
                     /locus_tag="RHV13_000633"
     CDS             88..1068
                     /locus_tag="RHV13_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HDV1240193.1"
                     /translation="MKIFIDDGSTNIKLAWLEDGDVKTLISPNSFKPEWSFSLLDDAA
                     PANYEIDGEKFSFDPLSADAVVTTETRYQYSDVNVVAIQHALQQTGLKAQPVDVIVTL
                     PISEYLDANNQKNKQNIERKKKNVMREVRVQGSDAFVIRSVSVLPESIPAGFSVLAGL
                     EDDESLLIVDLGGTTLDVSHVRSKMTGITKTWCDPNIGVSLITSGVKEQMAVHANTRV
                     SSFQADNIIVHRNEPDYLSRRIYNAEQRESIINVINERQKLLIKRVNDVISRFTDYTH
                     VMCVGGGAEIVAEAVKNLTKVPDERFYLSSSPQFDLVMGMIKMKGGVTNE"
     gene            1061..1477
                     /locus_tag="RHV13_000634"
     CDS             1061..1477
                     /locus_tag="RHV13_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HDV1240194.1"
                     /translation="MSDENKSRRCSFELFPDERTGDKIADELIANEKLKERGRFMRAM
                     LVTGAAFAAIDKRLPLLISELLTENTTLDDINKVISSVIPGAFSVEKKLLELLEKQSG
                     LHTSVDCSTPLTEQSLSRNDGEDQTRRNAENMFGDD"
     gene            complement(1479..2753)
                     /locus_tag="RHV13_000635"
     CDS             complement(1479..2753)
                     /locus_tag="RHV13_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDV1240195.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPWFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVEPYSIDE
                     MFIDLRGINHCISPEVFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRTLKLLGLQPVGEVWGVGRRLTEKLNALGINTALQLAQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAA
                     EKLRGERQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLPLPTQDSRDIIAAACRALNHV
                     WREGYRYMKAGVMLADFTPSGIAQPGLFDEIQPRKNSEKLMKTLDELNQSGKGKVWFA
                     GRGTAPEWQMKREMLSQCYTTKWRDIPLARLG"
     gene            complement(2753..3190)
                     /gene="umuD"
                     /locus_tag="RHV13_000636"
     CDS             complement(2753..3190)
                     /gene="umuD"
                     /locus_tag="RHV13_000636"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HDV1240196.1"
                     /translation="MSTVYHRPADPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLRPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
     gene            complement(3187..3435)
                     /locus_tag="RHV13_000637"
     CDS             complement(3187..3435)
                     /locus_tag="RHV13_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HDV1240197.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
     gene            3853..4755
                     /locus_tag="RHV13_000638"
     CDS             3853..4755
                     /locus_tag="RHV13_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HDV1240198.1"
                     /translation="MYFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSPENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEELPDDVRETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDIEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGDNFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDKVAHYGG"
     gene            4752..5063
                     /locus_tag="RHV13_000639"
     CDS             4752..5063
                     /locus_tag="RHV13_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1240199.1"
                     /translation="MKYDGDGRATARFFSGKGCRHAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGITLPSERRRLRYSTVGLTRYRT
                     R"
     gene            5139..5822
                     /locus_tag="RHV13_000640"
     CDS             5139..5822
                     /locus_tag="RHV13_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HDV1240200.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMFRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIENF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            5823..6044
                     /locus_tag="RHV13_000641"
     CDS             5823..6044
                     /locus_tag="RHV13_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1240201.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELEACFKD"
     gene            6058..6492
                     /locus_tag="RHV13_000642"
     CDS             6058..6492
                     /locus_tag="RHV13_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HDV1240202.1"
                     /translation="MYGTCETLCRELAVKYPGDMPLMLVIWSPEEIQALADGMDISLS
                     DHEIRTVLARLEDIPEDQRTESGISSGVAMEIINNVSANRQVTVPAELLASLIQTAEQ
                     ALWKREWAARDHGLAVPECVTRRQAVINQARTLLKNNRNEND"
     gene            6538..7314
                     /locus_tag="RHV13_000643"
     CDS             6538..7314
                     /locus_tag="RHV13_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1240203.1"
                     /translation="MNENTTLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIY
                     VLLDAPRLAMLLINRHGGVLPPLLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQV
                     FFPYAGEWLTEDEIRAVLDAVRDAVRSVSCRVAEDAQRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSECVEHILSSGLACDV
                     LRIPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRG"
     gene            7849..8151
                     /locus_tag="RHV13_000644"
     CDS             7849..8151
                     /locus_tag="RHV13_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDV1240204.1"
                     /translation="MMPEYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSA
                     DAAGIILTSLVINRQLWLYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            8198..8620
                     /locus_tag="RHV13_000645"
     CDS             8198..8620
                     /locus_tag="RHV13_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HDV1240205.1"
                     /translation="MYCTVKEIIREVLDTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFEHGADVSVVHDVVRELMEEKRASRQVTVPAVMLEKVLALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            8617..8808
                     /locus_tag="RHV13_000646"
     CDS             8617..8808
                     /locus_tag="RHV13_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1240206.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            8970..9314
                     /locus_tag="RHV13_000647"
     CDS             8970..9314
                     /locus_tag="RHV13_000647"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1240207.1"
                     /translation="MTLSPFLAVFYCAHFGSRLPGGIRSSGPTPTGSCWRTRQSPPNP
                     LARFRAAATVRCLKNQACSDRSGRAAPVAPETALPAHLTVCKATAPDGAVNPSAATPA
                     ACFPLWTFRGLE"
     gene            9558..9992
                     /locus_tag="RHV13_000648"
     CDS             9558..9992
                     /locus_tag="RHV13_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HDV1240208.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQQA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFREAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPK"
     gene            10084..10350
                     /locus_tag="RHV13_000649"
     CDS             10084..10350
                     /locus_tag="RHV13_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1240209.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAQDEIN
                     PDVLAMSGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            10415..11305
                     /locus_tag="RHV13_000650"
     CDS             10415..11305
                     /locus_tag="RHV13_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDV1240210.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFENPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQKGRQEGALEKALAIACQLQKMGMT
                     PEQIKQATGLSDDELKKITH"
     gene            11305..11550
                     /locus_tag="RHV13_000651"
     CDS             11305..11550
                     /locus_tag="RHV13_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014358130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1240211.1"
                     /translation="MRTTKPGKMPGFFVSRSRANSMTIAERLIQKGFDEGFKQGALEV
                     AREIACRLRDIGWPPERIQEVTGLSGEELKKLFPDEQ"
     gene            complement(11581..11832)
                     /locus_tag="RHV13_000652"
     CDS             complement(11581..11832)
                     /locus_tag="RHV13_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV1240212.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAGNV"
     gene            complement(11866..12063)
                     /locus_tag="RHV13_000653"
     CDS             complement(11866..12063)
                     /locus_tag="RHV13_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1240213.1"
                     /translation="MQGAPHTARLKKNDGTTMACGGHWHTTPAQVKTEKTARHLQAKK
                     VKFLHFFTLIYRADLAIVMIK"
     gene            12412..13260
                     /locus_tag="RHV13_000654"
     CDS             12412..13260
                     /locus_tag="RHV13_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HDV1240214.1"
                     /translation="MNQTLPTADLNTAGTTDVIPSVAIDRIIAQRNEGIALFMQAMEC
                     LATARKILLDASGDIFLYGFEDCVTDSVRCMDKPEEAKKNITRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEQGLIDVYRKLSW
                     DYRTNNPCRLGKKIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRN
                     SIGAQYGEFLGNGDNVGKLLEGEYFTVRGYQKGTVHIVFKRSDLVEKMNDIIARHYPG
                     ALPPRV"
     gene            complement(13346..13681)
                     /locus_tag="RHV13_000655"
     CDS             complement(13346..13681)
                     /locus_tag="RHV13_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HDV1240215.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKTAPKKTDSGR
                     KDGIDMNNN"
     gene            13915..14247
                     /gene="nikA"
                     /locus_tag="RHV13_000656"
     CDS             13915..14247
                     /gene="nikA"
                     /locus_tag="RHV13_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type relaxosome accessory protein NikA"
                     /protein_id="HDV1240216.1"
                     /translation="MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSA
                     YIRNAALNKRINSRTDDAFLKELMRLGRMQKHLFVQGKRTGDKEYAEVLVAITELTNT
                     LRKQLMEG"
     gene            14258..16957
                     /gene="nikB"
                     /locus_tag="RHV13_000657"
     CDS             14258..16957
                     /gene="nikB"
                     /locus_tag="RHV13_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type relaxase NikB"
                     /protein_id="HDV1240217.1"
                     /translation="MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQ
                     PHRSRFSRLVDYATRLRNESFVALVDVMKDGCEWVNFYGVTCFHNCTSLETAAADMEY
                     IARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVRHTLKSLGLADHQYVSAVHTD
                     TDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCWVHAPGNRI
                     VRKTAVERDRQNAWTRGKKQTFREYIAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSQ
                     MDGKFLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNP
                     DFIAADVRPEKIAETESLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRV
                     QHGHLVICDGYDHNQTPVRADSVWSLLTLDNVNQLDGGWQPVPTDIFLQVTPTERFRG
                     RRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGYSISHCSPQIEEMITQGEF
                     TWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEPQAGPFV
                     SAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAW
                     REQWRKPDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLK
                     EDIRDERQKLIADGKWYPPSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDKSRTTTT
                     DRCVVLCEPGGTPVYGNTGDLEARLQKNGSVRFRDRRTGEFVCTDYGDRVVFRNHHDR
                     NALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAWHNVTGRTGHEDYRITR
                     PDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG"
     gene            complement(16994..19285)
                     /locus_tag="RHV13_000658"
     CDS             complement(16994..19285)
                     /locus_tag="RHV13_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbC"
                     /protein_id="HDV1240218.1"
                     /translation="MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIW
                     PLALPACLLFTLITMLVFSLQRWRCPLRMPMTLECADPSQDRMIKRSLFSFWPTLFQY
                     EVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIMFFATTGGGKTETIFAWAINP
                     LCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEIILSGEKTR
                     PQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTS
                     AHTTSALGRMFITQKSMILARDLGYRLEGTDSDALEVKKYKGRFPYLCFLDEVGAYYT
                     DRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLMQNTGTKFAGRIVSEGSTA
                     RTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELHPGEFFS
                     IFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPE
                     NVSAIIGVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESA
                     LWETAVNTLKTTTREERRIRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNH
                     PTGRPVNGFHHKKTNRPDWDGMY"
     gene            complement(19278..20348)
                     /gene="trbB"
                     /locus_tag="RHV13_000659"
     CDS             complement(19278..20348)
                     /gene="trbB"
                     /locus_tag="RHV13_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbB"
                     /protein_id="HDV1240219.1"
                     /translation="MTIEYFATRIAKHISVQAIWPDGRTEIIAVLPRDALSGLVTRNN
                     QLIHIAFDAMGTRNRETVLIDKQEHNADQILTAIDSCLRLEYLMERRFSRTPLFRGII
                     ASVVLFVMATIGFSLFRYVDRVFWDDTTPEAVQTAGEPRLLPPHLNHTVPLNEGIQLP
                     VPPKKDVQVPEKTITSKNPEAAAARHNLATVLKRNADRGMFTINLSSGHERTLFAFLD
                     PACPNCRLLEPALKRLASDFNVVIYPVSVIGGEESTDRVAPLLCEKDAQKRAAGWHRL
                     YSADNGMMTPSEETTPADETCLKAARAAIDVNNVAFRKFGFAGTPWVLSDTGWHLPTG
                     ILQETGTLNLFLKTTDSESGHE"
     gene            complement(20367..21575)
                     /gene="trbA"
                     /locus_tag="RHV13_000660"
     CDS             complement(20367..21575)
                     /gene="trbA"
                     /locus_tag="RHV13_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbA"
                     /protein_id="HDV1240220.1"
                     /translation="MSYNRQPVAEDPMQIWGAVGVLLILLLFVIWLFLPEVVYASCLI
                     LHTLWGLVDWGPFHNYAAPRYNLLAMTGNNAANISYSQWVNVMEQTIGILWMYLLPVT
                     LWCLWEWYQHPGQSRFTRRPVDITRLPHIFASLSPAIAPVLADGDPEKLFHGGKRPER
                     RVALTPEAFVEQHTLITNMQLDVAAARRCFMAQLGKPLTSWKDMAPHEKALFAIFGLQ
                     YFLDDRKAALKLMDTLNLSCRIKSKRDSGKFCTPVYSLAKSAFQRVIKSNGAQQWLKQ
                     HRYVRSGLVWLYAHDLRLTPPNWIWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     EAEAMRFGLPCPEPCVDEAVEGLRRDMLSLGLIWDEPQPDRDRKRRILTNWSLTDDIL
                     PRTPATDNEF"
     gene            21867..22019
                     /locus_tag="RHV13_000661"
     CDS             21867..22019
                     /locus_tag="RHV13_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HDV1240221.1"
                     /translation="MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATL
                     AYEVKR"
     gene            complement(22091..22342)
                     /locus_tag="RHV13_000662"
     CDS             complement(22091..22342)
                     /locus_tag="RHV13_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1240222.1"
                     /translation="MSEKWSKEFDSCFQVGSIEEIADALEKRSGSEENALAMLAMTAF
                     TLMTRRGICEMQNVAPDGKGIRLELIGFRENETIPDTLH"
     gene            22642..22938
                     /locus_tag="RHV13_000663"
     CDS             22642..22938
                     /locus_tag="RHV13_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000115013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1240223.1"
                     /translation="MSALRHVLSGILFSGKNRLQVLAKTSGGAGFGALVGEELSITDT
                     IETVVTGSVRANNGVLSPVMEKRMINLLIIVLRAVVAVANALIAVLELIRELID"
     gene            complement(23003..23179)
                     /locus_tag="RHV13_000664"
     CDS             complement(23003..23179)
                     /locus_tag="RHV13_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1240224.1"
                     /translation="MNLVDAFVKKVISGPYKEYGKWWIDVEYISWGVPGKTRLMFESK
                     EQALEVKEGYKFLT"
     gene            23571..23780
                     /locus_tag="RHV13_000665"
     CDS             23571..23780
                     /locus_tag="RHV13_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HDV1240225.1"
                     /translation="MSMPPAIANTFLFEMMKSKSKDATLAAIYALGEGRCQAENITRE
                     LHRLSQSDDMEIKIAAIKALGRIYR"
     gene            complement(23852..24514)
                     /gene="excA"
                     /locus_tag="RHV13_000666"
     CDS             complement(23852..24514)
                     /gene="excA"
                     /locus_tag="RHV13_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HDV1240226.1"
                     /translation="MKDKKETPDRFPTWWLLYYVLRKAYFFLGIPFFLFCALTSTLML
                     FSSRYYGDNIEDYVVTFGSWFLLLAPGIWMYSRAKTRREKIRKVVQTIKESGFYSPEK
                     GYEGLSLTQGAYFGIDLKNGTMLYVRIYPGNIMDVIGFDIHNFTRTVTDDKTLEIHTK
                     YINLPMVPIPSWCTHPETASNTMHAMASRGYDYPVDFPRLIQEKRKEWEQIAGMPVAE
                     VF"
     gene            complement(24579..26741)
                     /locus_tag="RHV13_000667"
     CDS             complement(24579..26741)
                     /locus_tag="RHV13_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HDV1240227.1"
                     /translation="MKILLRALCAGLAISSLPAMASVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLSTSAPTLIGSMFGAFNSIIAVLAVVWFMFIGIRHVVRSGHQGQVFST
                     GRDIVGTLSVVAGFLMIVPTGNGWSIAQLIMLWGASIMGVGSANVMVQLAADNIANGY
                     SMTVQPVQASTQTAARGIFEMELCKYAVNAGLNDFNQTAKSSTSLMTESAKTASGNYT
                     VTVSNGSGICGSASLSVEGNGTTDQSTIGKFFNPFSKNEYSGVISAQRAAMDNMISDM
                     DNAASEFVTTFLEKRNSGNGTLPDIETRIQRAADEYERAVQKSLPTDNGEQSRKEALK
                     SYLTTYGWVTLGAWYQTFATANQRLAELADRAPAVTSMSSLGEVGDTDLFSAVMSAYK
                     TQLQNTTYTPPLGTVTSANEQRMANAQDPKSALSEMMSFFVYRTNWIATEISGTGTTS
                     DQVNPLIKMKNIGDYTLGTTEILWSGYTTVRVLATVGDNSIFGKVANLFSGLPKGLVA
                     LLDAAAPPIYFLLFLLFCAGFSLSIYLPFIPFIFWMTGIGNWIVSVLIGCTAGPLWAA
                     THLGTSEDRGSRAAYGYIYLIDSMIRPPIMVFGFFFASVAIIAVGTILNALFGAALVN
                     VQFNSLTGIFSLAGFLLIYARICTTSVAAIFALQAYLPDHVINFLGGREGANTLGSMA
                     NSVKEIFIGGSRNIRHTPGMKTDRLKDKGSDDKDGIKG"
     gene            complement(26838..27422)
                     /gene="traX"
                     /locus_tag="RHV13_000668"
     CDS             complement(26838..27422)
                     /gene="traX"
                     /locus_tag="RHV13_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraX"
                     /protein_id="HDV1240228.1"
                     /translation="MNKLPENSDQTKNVKKSPSITRIATRGIINIFVPLMETRLITNS
                     VQHMLEQQKRRLDRFRQVNNKKAQLHLTWEEALQASHMSVDDLDRRFRRRRTVWRFCC
                     WSLLAIALFLSGMLFAASSLPLTTLVRAISTLVLILSGVALCASRALIVTYRLWQLHE
                     RKVSEPEQGTFRDFLNDRNGWRNATLIAVTSKQY"
     gene            complement(27451..28653)
                     /gene="traW"
                     /locus_tag="RHV13_000669"
     CDS             complement(27451..28653)
                     /gene="traW"
                     /locus_tag="RHV13_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraW"
                     /protein_id="HDV1240229.1"
                     /translation="MQRKTLLAALIATLSGTACQAHAYSVTVVASRPVEEQVIPRMEA
                     IKDVLGNILSTQTATGTAINQNSEKLASVIAQNGQATRQQMIFSNETQRLEEARKSFT
                     VPDSICSESASGIATESKSASASAASKLSKGGGVSNRSIRDRLASAANSPVREAYDGA
                     AIHASYCTEAEYARFGGTAVCPSVGEIPGGDSQVRSIYHGAGTADTPAALTWDQKQID
                     AATAYMKNTSRPSAGRALGKGEVNTQSGRTYVGLQNEYNGIIDSASNPQLTLIADSTP
                     NESTRKALAETLQSDSAAAYFDQVASPEAKARGYMSTREFEAFEAGRRYANTAYLVDL
                     QEMQGDNLLRELVRITAQMNWQLNDLKEQIRQGNVISGQQLALTARQYYEKQLGSLEK
                     TINQANAR"
     gene            complement(28620..29234)
                     /gene="traV"
                     /locus_tag="RHV13_000670"
     CDS             complement(28620..29234)
                     /gene="traV"
                     /locus_tag="RHV13_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraV"
                     /protein_id="HDV1240230.1"
                     /translation="MCTIHITPRCRRPARGQVFLKKLEVKGDSFSFTLPSRVFRLHPA
                     PNPRVIYASDARTQGKVTGMRLLLPSSTDELITTAIWQVSVPDEAPFDVQHVIQWILP
                     SPLASLMSDDTWLWPSVPGTPALSAGDWPVMDTDPSTLRRVRRRSFSIIDNRNRKGYI
                     TRRFQCFPLAPLPENRKYDSLPDLVLHPEQETIKCKEKHYSQPL"
     gene            complement(29234..32278)
                     /gene="traU"
                     /locus_tag="RHV13_000671"
     CDS             complement(29234..32278)
                     /gene="traU"
                     /locus_tag="RHV13_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraU"
                     /protein_id="HDV1240231.1"
                     /translation="MNINRLIFTIEDCLNTLSRFSVASSFVEYCDLRTVIGLDRQDRE
                     RRPWLNSPYIAATKRGEYLSVFEVSGAFREMDEASDQTGPGSLESLITSMSDSLNTAY
                     KNSGHKISFVFERDPDMGKEEIEDMVAPQKRSLANTGIQLQDVVDEKVTTLSPWLVRE
                     RCWLAIWSGPDLISNSDRTAHDELVRRLAERVPKARFAQSPWQWTLSALKIRHEAFLD
                     NVEQALRHSSDGLILRLLDIHEVGREIRRQTERHSTPRNWQPHLPEDAQPAGYRWTDD
                     ESVLHAPSLHLQLFNTQVTTQGNLVQAGGLWHGMVSITLPPQNLQTFNELVRAVPRAV
                     PWRIRMDLMPGGMKALNLKKTLLTYSSFISAVRPMYESVMALAATDEKEPVCIMTIMA
                     STWGKTREICTRNQAILKSAIEGWGVCGTTTTFGDPRRAWVNTILAASGGSGPVPLYP
                     PLSHAISLFPLNRAGSVWRGKGNLMLHTEDGSAIEVGLASSQQNKHTELAPGDPGLGK
                     SVLINTLSEIQISSAQKNLPFIAYIDKGYSAQGLVQLIRDSLPPERKDEAVGIILSND
                     PEYTRNLFDVMYGAKKPITPEKNFMSSVLCALCVDTGTGQPCNPGDTRQIINQLIELA
                     FKEYGENNPRLYRASTEELVDSALQDSGLYEKHDATWWARSTWFEVRDMLHNAGYIMA
                     AQRAHYQAMPQLPEVSSMLGHTSLRDVFGTVQRDGSNELLLDYIRRALEQGHNDYPMI
                     SGYTRFMINPETRVIAVDLNNVAGDKTPAGRLKTGIMYLLAGQIAGGDFTLPQYRDEV
                     LKQLPREYHEIALKRINQLDQEVKTKVYDELHNARGIDFIWENLDTQEREQRKFAIRT
                     VLSTQYLRDYPESVLKSANTLWLLRYKPEDIPVLRDNFNVPEFMLKRFLKMPEGPAPD
                     GSGVPVLGVFRVKSGTLARILKFTVGPLELWALNSSPKDSALRKTLTNKLGSVRARKI
                     LAENFPRGSATSLIEHRAGQHNSDNVIEELASELIRKQGYNL"
     gene            complement(32368..33168)
                     /gene="traT"
                     /locus_tag="RHV13_000672"
     CDS             complement(32368..33168)
                     /gene="traT"
                     /locus_tag="RHV13_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraT"
                     /protein_id="HDV1240232.1"
                     /translation="MQHSIKDLWLYPFPEIDVVHTQEPLLPEPELTTPGRCICCRQNV
                     RHRFRLDDSWPLRQLTDTISDTRVRLNKATEHLDKLKKRGEPVATGEKEKYNTAVKAA
                     ERALEQARLSARRLSLRHVQKAEITSTESLSEKEQELFHEDGPPYSLCAFCHAWHSLN
                     GYAAAQGVMVWLPDLHPSTVVALNRRSLQEVFSNDKFRVRRGREALSALMQNRLAVED
                     KFRSFRPADFADVFRRYPPSGRSPLREKMNGIALILTPDSFIKKEYVD"
     gene            complement(33152..33340)
                     /gene="traS"
                     /locus_tag="RHV13_000673"
     CDS             complement(33152..33340)
                     /gene="traS"
                     /locus_tag="RHV13_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative conjugal transfer protein TraS"
                     /protein_id="HDV1240233.1"
                     /translation="MSAWSPEHITLRRTENGNVIAHDSRDDQEFIFAECCSDEIANII
                     LEAVKRYTSTEPAHATQH"
     gene            complement(33404..33808)
                     /gene="traR"
                     /locus_tag="RHV13_000674"
     CDS             complement(33404..33808)
                     /gene="traR"
                     /locus_tag="RHV13_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraR"
                     /protein_id="HDV1240234.1"
                     /translation="MSRIRVVAARLYAAFIHAQLFVMSCRSKLTKFLLLIPMMLLPKA
                     VLADGDLADMVRNVEQGAKTAQSSSLTIAQFIGVIAFLGGLIGLKKVGKQGGMGLAGC
                     ILSIVIGALLAAGPEMMSRSQKQLGISSISIG"
     gene            complement(33859..34386)
                     /gene="traQ"
                     /locus_tag="RHV13_000675"
     CDS             complement(33859..34386)
                     /gene="traQ"
                     /locus_tag="RHV13_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HDV1240235.1"
                     /translation="MNMDALTAIENFASSIFSAGMDFLFTWGEFIGVISMITLFARAR
                     SAGPVKMSPGKFIAGMLTSCMLVSLPAMINAGGVQMGFRADSFGPIAYVQPQTFGAAA
                     GAANAVLSLAKLAGVGFVMNGISIWRKAGLDGHTALSASESVSKGNVKFIAGVLLVFI
                     DRVLNALLASIGIVF"
     gene            complement(34386..35090)
                     /gene="traP"
                     /locus_tag="RHV13_000676"
     CDS             complement(34386..35090)
                     /gene="traP"
                     /locus_tag="RHV13_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraP"
                     /protein_id="HDV1240236.1"
                     /translation="MKPETEIDETGSFGEPEEKPAPFWKRSVWGISVATWGLCAVVLL
                     AAIWYLFLRAPSETGMPPFNDADAGVQTWQTTQESSPSVQSTETMTVRTGEDMSQLAR
                     DVKTELDNRDEKIQATLNMLHDSINKLGEAIKKDEEYAQETRRQLDDIRSRLNGIMTQ
                     KSVTESSSTPHPAKKKTSSVLNGMKIMSMETGMAWIRWQGSTWAVREGQTLGNVVIQR
                     IDPTTRTIITSAGTLR"
     gene            complement(35090..36379)
                     /gene="traO"
                     /locus_tag="RHV13_000677"
     CDS             complement(35090..36379)
                     /gene="traO"
                     /locus_tag="RHV13_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HDV1240237.1"
                     /translation="MSAEQDAGKSGKKLAALLGLSSIILFGGSYIAFSKVSGSNSDMQ
                     SAVNINSAASGGTRSVTETPHYRELLRADNERGAAAAARNNQTFIASLPQGLDIPDTP
                     EKQQQPAAKPENYAYRQASGTPQEDRAASEKRMERLQKLIVRIKDQHPAGSTPTIATT
                     MWNKSPAETTGQNGTQQFALQNASLSTPVAEKGIQLIPALTRIPAYIDTAVDSDNPSS
                     KVIATIPAGPWAGATLFSPGVKLVGNGVEIHFDRMSWNGMDLKVNAYAQREDNLMSSV
                     ASNVNTRWFKHIILPSVLGGVGSIGTLYKDANTQVIQGNYGTVTGRVGMPSGEAVAGV
                     IAGGMAERGSQILTRQAESEPYKQVEVYQHEVVSILFVDPVMTNDARSSSLSSGISPS
                     VNRTSQAEQRSQARMQTAMEQRKAVMQRRYDEQPETP"
     gene            complement(36382..37365)
                     /gene="traN"
                     /locus_tag="RHV13_000678"
     CDS             complement(36382..37365)
                     /gene="traN"
                     /locus_tag="RHV13_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraN"
                     /protein_id="HDV1240238.1"
                     /translation="MQSRYLLSTLLLVCSAATSADNAGWQNARTPQTTNSASDHVQNA
                     SQNTGGVPATLVKGELPAPGQASPLVQDAARLDSELSADEIRSLRSLMADNERAINAP
                     ITSVVPRISSLTVNLSPGASLPLVRTAMNNLSVVTFTDINGSPWPQSDPPYNAAPKLF
                     DVQYNENMVTITPLRPWASGNISVYLKGLSVPVILNVTSGETDTPSSSQEMDSRLDLR
                     IPRQGPTSPVVSIPTDKIALHDATLQAFLDGIPPRDPSVKRLKFTGNVPDTTIWQHGD
                     DLLVRSRAILRDEFEQTLSSADGTHLWKLPVTPLLTFSVNGQSVHVTPELE"
     gene            complement(37376..38068)
                     /locus_tag="RHV13_000679"
     CDS             complement(37376..38068)
                     /locus_tag="RHV13_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HDV1240239.1"
                     /translation="MTENTELSSPSHPAPDPEILKSTIRAMKQSEQRANNVPALIKAL
                     LCTGTCLLISITGNAIQYWHSTNVEREYFATDNGRLVRLAPTNQPAWSQNDAMAFGSQ
                     ALATAFNLDFVHYRSQISSLSPRFSDEGFAGYVNALQASNILETIKKEKMNLTATTGA
                     GVLVRQGQMSDGVWFWTFQYPVRMRLVGQTTSKPEQSFVFEITIQRVDPRLKPSGMEI
                     RQMISRNAGPNS"
     gene            complement(38065..38412)
                     /locus_tag="RHV13_000680"
     CDS             complement(38065..38412)
                     /locus_tag="RHV13_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001629514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDV1240240.1"
                     /translation="MNMHKSLVAVLGLILISSTASAATCRAGSAAQAGSNTGYERARA
                     AADAWSQRENDVSSSLQSCLSRIKKISINLPQFPSLDDILSQLENQVCDAVVDKVNEK
                     LPGNIDPWKDYNL"
     gene            complement(38430..42197)
                     /locus_tag="RHV13_000681"
     CDS             complement(38430..42197)
                     /locus_tag="RHV13_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDV1240241.1"
                     /translation="MPSVSRYRTWLAVPADEIEDLKKAHPPMNGHTPVIWDKEHKLWF
                     ARPGADLSRLDRWLPRPQDVSMNGSDPVTEFAQVLENAGLVLKELPVMDGKIHRVPTA
                     DDKKGQKSGAYRGFLDGRPAGWYRDYRSADDSPITWTFSGGEQTDPRARLHLKAHSMQ
                     RREDAERELKAQYNRQAAYARRYVNKWPQATAHEYLTRKGIQAAPGVRVNNKNELVIP
                     FSNRNGAIRSYQRIPVTGGKDARILKDSEKTGNWFALGTPRNGQPVLFAEGYATAASL
                     HEATGLPVLMTVDGGNMIAVAENARQKWTQSPFIFCADNDHAIRVNKGIVSATKAAEL
                     TGGTVIFPAFTDAEKAQGLTDFNDLDASRGRAAFQHVINAQLEHIGVSTPNSNTPEIR
                     EALVIGNLVFTPVHTEEKTMTPTEYPETSPDTGHSHDQGPSLPSATQQEQPAASSSNI
                     ADETQSFTSHATENNGKDERHADNVQAQTESSVVQDSPAESPASPESTTASAPDEPAH
                     PAEPPEKVVSVATDKDWREFEAELSQPEKNESQQESGTSPEIPAPAAAPASPEDSPSS
                     ETMNESAEPSPSPDAVKEEMTVMTEPDDKQSTGISPDTPREEAVSHVTEDIPLQPEPF
                     LPESDGPEEYENYSAYQELMNNENPEHLQENSDMPSQPDTDHVQADESELQATTDTAE
                     PAVRDEPNPQQPVQSTSPSDNTSSFLDKARGFFTRKKTDSQAHENSDPTPSPETTTTT
                     PTPDSIVYAPERPDAPISLNLDEIIKSLKGEKRADRTVLYKLDGKPAFIDRVNRLEMV
                     DGASNDDRSVLAALAVATNFYGGVIELTGSDAFKQKAMRLIIEHNINVRMKHADQRLE
                     LDTLRKEMAVGKDAVVTHQPTPELNRNTPEQPVVPDPVQEKEATQSPASPVAPEASTV
                     STVPASSPAEPGKTADAAQGEEPPGKLRPGESVTAVLHNFGRAEYAPGKGESFFVELK
                     NRSGSKLYWGEQLESLVKNHQKGDVVTLTLQNREQFILPGEQKARFRNKWSMESVTNG
                     ISVSHDNPDKGQRIQAIPVETFMKVAAQISQGWPEEMKALRMPENVGSHLFIGEDRHP
                     VSAPQNANQVTEITSAAPDKLTPVLGSVDKDTRELNLLLVQSADEHLQGVVRLNGTLY
                     PALATPSADNSQLVINALTDKGLRFAGYGEAVNHDADSTNRPAPELMQFHLKTREEPL
                     FAAVYTPEKQPDALYRNLGFEQSWQQWSNSQKPEDRQEKTLHQDLSHSPGR"
     gene            complement(42287..42838)
                     /locus_tag="RHV13_000682"
     CDS             complement(42287..42838)
                     /locus_tag="RHV13_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HDV1240242.1"
                     /translation="MSGKKTIVTLLRVSLLACPLLFTTPSFAMIDTPSVKVGFSPEGS
                     ASALVLDTINSAESSIRMMAYSFTDPDVMHALAKAKKRGVDVRIVVDDKGNTNRASQE
                     AMKYINLLDIPLRTVDAFPIHHDKVIIVDGNTVETGSYNFSRAAARKNSENVVVLKNM
                     PDVAAQYLEHWQDRWNKGTDWKP"
     gene            complement(42853..43143)
                     /locus_tag="RHV13_000683"
     CDS             complement(42853..43143)
                     /locus_tag="RHV13_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDV1240243.1"
                     /translation="MKREGFWRYATPPVNIQGVPLPFTMIYLFICPFPSKTTFWICTG
                     IILFFVILDRSGWTVRTICTRIFSILRGAIASGRPWWYRHHTESPRDWTGLD"
     gene            complement(43140..44288)
                     /gene="traJ"
                     /locus_tag="RHV13_000684"
     CDS             complement(43140..44288)
                     /gene="traJ"
                     /locus_tag="RHV13_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HDV1240244.1"
                     /translation="MNIPDEFGLFPFQRFTADELRHFFVWCAAHKVSDVDLTGGSPVS
                     VSRFGRRVRCSSATLPTTLMSSLIDELFGREVIPRVLAGNPVDRTIQINGDASGRYGL
                     KRGERIRLRSHLIQGTSGAEEKAISITMRVIPTEIPDILSMNIEPDLLEAMVCKSGLG
                     FVCGETGSGKSTLCSALYRYIMDNFPDAKIVTYEDPVEYILGNENDLLPPHQAEIGRD
                     VVSFAAGLRSAVRRNPEIIGVGEIRDNETADAAVQAGNTGHYCLSTMHTKSPGETLAR
                     LLGLFPPVIRDSMAWAVLSLLQFILVQVLVRTNDGGRKAVREYIVINDELRDNLSGMP
                     HAEWGHHIDAIIRQEKRRIRDQILEMYIRNEVDRREAILFIPPGELRS"
     gene            complement(44285..45103)
                     /gene="traI"
                     /locus_tag="RHV13_000685"
     CDS             complement(44285..45103)
                     /gene="traI"
                     /locus_tag="RHV13_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraI"
                     /protein_id="HDV1240245.1"
                     /translation="MKALTSLLLPVILLTGCSSSPTISPGGVNTPPPDINAWLKPRSE
                     KPDSLSETRWKMLTDAGKTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISPEG
                     WLPPVIDEAQDVAHITPDQIRTSSRVWTIIRPERFVSNPPGWRDWLLRGLSTTATPGT
                     EGSVVPEDSVQRKVWETALRQGWQEGRQNADLTLEANQKTLTRDYRGMMLYSLLWRQG
                     MITRPDVSDQMQTVTGDGKKLVTGDRVRRLKNHAEFNLQKSHWRPLIGTEGGSR"
     gene            complement(45100..45558)
                     /gene="traH"
                     /locus_tag="RHV13_000686"
     CDS             complement(45100..45558)
                     /gene="traH"
                     /locus_tag="RHV13_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraH"
                     /protein_id="HDV1240246.1"
                     /translation="MNRIIPAGCLAALFLAGCNSPSQHTPVPRNYGAASDVAVTRHTQ
                     YTLWDKGVFSGQLPVSTGSHLLTANSDRLSFDWEGDAIELLNELARVRGMQFNYNGVR
                     LPLPVNLHVRDMTFSNTLRLIEAQTAWRATIHQYPGLLQVSFMQPENRKK"
BASE COUNT        10597 a        11481 c        12215 g        11776 t
ORIGIN      
        1 accgtttttt cattataatt gaatatacat tgcatgtatg aagcatgttt gttttttcta
       61 tttgtttatg agcaacggag actggttatg aaaatcttta ttgatgatgg ttctaccaac
      121 attaaacttg cctggttgga ggatggtgac gtgaaaacgt taatcagccc taacagcttt
      181 aaaccggaat ggtcttttag tttactggat gatgctgctc cggctaatta cgagatcgac
      241 ggcgagaagt tttcatttga tccattaagt gctgatgctg tcgtaacgac tgaaacacgc
      301 tatcagtaca gtgatgtgaa tgttgtggcc attcagcatg cgctacagca gaccggcctg
      361 aaagcgcagc ctgttgatgt catcgtcaca ttgccgatct ctgaatacct tgacgccaac
      421 aatcagaaaa ataagcaaaa cattgaaaga aagaaaaaaa atgtgatgcg tgaagtacgg
      481 gtgcagggga gtgatgcatt tgttattcgc agtgtttccg ttcttcctga aagtattccc
      541 gctggtttca gtgttcttgc ggggcttgag gatgatgaat cccttttgat cgtagatctc
      601 ggtggtacaa ctcttgatgt gtcgcatgtt cgtagcaaaa tgacagggat tacaaaaacc
      661 tggtgtgatc cgaatatcgg cgtgtccctg attacatccg gggttaagga acagatggct
      721 gtacatgcta atacaagagt gagctcattc caggctgaca atatcattgt gcaccgtaat
      781 gaacctgact acctttcccg tcgcatttat aatgctgaac agcgtgagtc tatcattaat
      841 gttatcaatg aacggcagaa gttgcttatt aaacgagtta atgatgtcat cagccgtttc
      901 accgactata cacatgtaat gtgtgttggt ggtggggctg aaattgtggc cgaggctgtg
      961 aagaacctga ctaaggttcc tgatgagcgc ttttatctga gctcatcccc gcagtttgat
     1021 ttagtcatgg gaatgataaa aatgaaaggt ggtgtgacta atgagtgatg aaaataaatc
     1081 cagacgctgc tcatttgaac ttttcccgga tgaacggaca ggagataaga tagcagatga
     1141 gctcattgca aatgaaaaac tgaaggagcg gggacgtttc atgcgcgcga tgttagttac
     1201 tggtgcggcg tttgcagcta tagataaacg tcttcctttg ctgatatcgg agttactgac
     1261 agaaaatacg actctggatg atattaataa ggtgatttcc agcgtaatac caggtgcttt
     1321 ttcggttgaa aagaaattgc ttgaattact ggaaaaacaa tctggtctcc atacgtctgt
     1381 ggattgcagt actcctctta cagagcagag tctgtcgaga aatgacggtg aagaccagac
     1441 cagaagaaac gctgaaaata tgttcggaga tgactgaact aacccagcct ggccagggga
     1501 atatctcgcc atttagttgt ataacactga ctcaacattt cccgtttcat ttgccattca
     1561 ggggcggttc ctcgccccgc aaaccacact ttcccttttc ccgactggtt cagttcatcg
     1621 agtgttttca ttaacttttc actgttttta cggggctgga tttcatcaaa taatcccggc
     1681 tgcgctatac ccgatggtgt gaaatcagcc agcatgactc ctgccttcat atagcggtac
     1741 ccttcacgcc agacatggtt taaggctctg catgcggcgg caataatgtc ccggctgtcc
     1801 tgtgtgggca atggaagctt ttccacagcg gcattgctgt aacagggttc ttttactgca
     1861 aagggggatg tccgtacaaa tgtcgtcacc tgccggcaat actgacgctc cccacgtagt
     1921 ttctctgcgg cccgctctgc atactgaaca acagcctggt gcatggcatc tttgtctgtg
     1981 attcgttcac caaaactgcg actacagaca atctgctgtt ttgccggtgg tgcttcttcc
     2041 agggatatgc aggactcgcc gttgagttcg cgtaccgtac gctcaagaat gacgctgaag
     2101 tttttccgga tgaatgccgt gttagcctgc gccagctgca gtgctgtgtt aatacccagc
     2161 gcattcagct tttccgtcag tctgcgtcct actccccaga cctcaccaac tggctgcagc
     2221 cccagtagct tcaaggtccg attacggttt tctgccgtca gcgcgaccac tccggaaaac
     2281 tgtggccatt gctttgttgc ccactgtgca cttttagcca gcgtttttgt aggcgcaatg
     2341 cccaccccca tggtgagtcc tgtccagctc tttacctgtt ccctgagctg atgaccaaaa
     2401 acctccggag agatgcaatg atttatcccc cgcaaatcaa tgaacatttc atcaattgag
     2461 tagggctcaa ctgcgggaga aagcgactcc agaacagcca tgacccgttg gctcatgctg
     2521 tggtacagcg cataattgct ggaaaataca tgtattttct tctccaggcg catctgtctc
     2581 acctgaaacc agggctgccc cattctgatg ccaagggctt ttgcctccgg gctgcgcgcg
     2641 atcacacagc catcgttatt gctgagtacg atgaccggtt cgttgcgaag gtccgggcgg
     2701 aaaacttttt cacatgaggc gtagaaactg ttgatatcag ccagtgcaaa catcagcgta
     2761 actcccgcgt cctgtgtatc acgtgagtga caacaccaaa aatacagatg ttttccggat
     2821 acagtgtgcg gaattccggg ctgtctgaaa ccggctccag tgccgggcgt gggcgcaaca
     2881 gcagtcgttt gacggtgaac tcaccgtcga tctcagcgat aacgatgtcc ccgtgttgtg
     2941 gtttctcggc cctgtccact accagcagat caccattctg cacgccagcc tggttcatcg
     3001 attcaccgct ggcgcgcaga aagaaggtgg ctgcaggtct gctgatgcaa tagctgttca
     3061 gatccagttc ctgctcagca taatcagtgg caggcgaagg aaaaccggcc tggcaacgat
     3121 cggcaaacaa cggtcgcacg taactgtcat cgcctgaagg gtcagccgga cggtgataaa
     3181 cggtactcat gctgcaggca gccagctgtc gtcctgccag atttcttcca gcagagacag
     3241 gatacgttct ttatccttgt cgttgcgggc tcctgtgaca gatacagacg gggcgctgcc
     3301 ctggtctata cgcagccaga tttccgggta gtgtggtttc agacgttgtt cgatttcatt
     3361 ctgtagcgcc tgaagtgtgc ctgatttgag gttttttgtg ctctggcggt cgaaaagaat
     3421 ttcaatgcgg atcatgttct catctccctt ttctattgct gtatgcatat acagtattct
     3481 aataactgaa tccacacaca gtcaaatgtc attattgtga ctaaaaaacg ctcaattctc
     3541 tgggagtgtg gctgtatgcc tgctgtggac tgctgtgtcc agcttatcca cagcattttg
     3601 cgcacggttc tgtgaataaa atacctggtt acccaggccg tgccggcacg ttaaccgggc
     3661 tgcatccgat gcaagtgtgt cgctgtcgcc ggcctcctca cccggtcacg tttcgtcgtt
     3721 cctcctccac gcgctgcggc ttcggggccg cacctgcatt cgtatgcggt cgcccggtta
     3781 caggtgcggc acggcctgat ggaggccgca tgtgagagga gaattcccat gccaaactgg
     3841 tgctcgaatc gtatgtattt ttctggtgaa ccagcacaga tcgctgagat taaacgactg
     3901 gccagcggtg cagtcacacc gctttatcgc cgcgccacaa atgaaggtat tcagctgttt
     3961 ctggccggaa gtgccggact tctgcagatc actgaaaata tccgctctga gcagtgcccc
     4021 ggagtgacgg ctgccggacg tggtgctgta tcaccggaaa atatcgcgtt cacccgctgg
     4081 ctgacacacc tgcagaacgg tgtactgctg gatgagcaaa actgcctgat gctgcatgaa
     4141 ctctggctgc agagcggtac tggacagcgt cgctgggaag agttaccgga tgatgtcagg
     4201 gaaactatca ctgtgcattt caccgcaaaa agaggtgact ggtgtgacat ctggggtaac
     4261 gaggatgtat cggtgtggtg gaaccgtctg tgtgacaacg tactgccgga aaaaaccatg
     4321 ccgtttgacc tgctgacggt tttgccgacc cgcctggata ttgaagtgaa tggctttaac
     4381 ggcggtgttc tgaacggtgt tccttctgca tatcactggt atacagaacg gtatggcgtg
     4441 aagtggcctt gtgggtatga cctgaatatc agtagtcagg gagacaactt cattcaggtt
     4501 gatttcgaca cgccgtggtg tcagccggaa agcgacgtta ttgcagaatt aagccgccgt
     4561 ttcagctgca cgctggagca ctggtatgcc gaacagggct gtgatttctg tggctggcag
     4621 ttgtatgagc gcggagagct tgttgatgtg ctgtgggggg agctggaatg gtcttccccg
     4681 acagatgatg atgagctgcc ggaagtcacc ggacctgcgt ggatagtcga caaggtggcg
     4741 cattacggtg gatgaagtat gacggagacg ggcgggcaac cgcccgtttc ttttccggta
     4801 aaggatgtcg ccatgctcct ctttttactg cgccggctga cgcggcgcgg cataaacgct
     4861 gcctgtggtc agtgtcccgc gtccggcggg cacgggacgg gcgcttttac cgctcccggc
     4921 tggcgtcggt gacagtgtac gccagcccgt cgcccttttc tgatgaacgc ccttcaagcc
     4981 gctttcgcgg cataaccttg ccgtcagaaa gacgacggct gcggtattcc acggtcggcc
     5041 tgacccgtta ccggacgcgg tgaacagccc acaggcagcg gggaacgggc accacagggg
     5101 tgccctcccg gtgccctctg taagagaagg agtttcgtat gtcccgtttt gtcctcggta
     5161 actgcatcga tgttatggcc cgtattcctg ataacgccat tgatttcatc ctcaccgacc
     5221 cgccatatct cgtcggtttc cgtgaccgtt ccgggcgcac catcgccggc gataaaaccg
     5281 atgagtggct gcaaccggcc tgtaatgaaa tgttccgcgt gctgaaaaaa gacgcgttaa
     5341 tggtgagctt ctacggctgg aaccgcgtcg atcgctttat ggccgcctgg aaaaatgcgg
     5401 gattcagcgt tgttggtcac ctggtcttca ccaaaaacta cacatcgaag gccgcatatg
     5461 tgggctatcg ccacgaatgc gcctacatcc tggcaaaagg ccgtccacgt ctgccacaaa
     5521 acccgctgcc ggacgtgctg ggctggaaat attcgggcaa tcgccatcac ccgacggaaa
     5581 agcccgttac cagcctgcaa ccgctgattg agaacttcac acacccgaac gcaattgtgc
     5641 tggacccgtt tgcaggcagc ggctcaacct gcgtcgccgc cctccagtcc ggacgccggt
     5701 atatcggtat cgagctgctt gagcagtatc accgtgccgg gcagcaacgc cttgccgccg
     5761 tgcaacgggc catgcagcag ggggccgcga atgatgactg gtttatgccg gaggctgcgt
     5821 aaatgaacta tgcaggacac gaaaaactgc gcgccgaagt ggcggaggtg gccaatgcca
     5881 tgtgcgacct gcgtacaacc atgaatgaga tggagcagcg gtacagcttt aatgccgaca
     5941 ccctgccgga acgtctggtg cgtcagacgc tgtttcgcgc aaaccgcctc ctgatggagg
     6001 catataccga aattctggaa ctggaagcgt gctttaaaga ttgagaagga gacgagaatg
     6061 tacggaacat gcgaaacgct atgccgggag ctggcagtaa agtatccggg agacatgccg
     6121 ctgatgctgg ttatctggtc cccggaagag attcaggccc tcgctgacgg aatggatatt
     6181 tccctgtccg atcatgaaat cagaaccgtc ctggcgcgcc tggaggacat cccggaagac
     6241 cagcggactg aatccggtat ttcttccggc gtggcgatgg agatcatcaa taacgtgagc
     6301 gcaaaccgcc aggtgaccgt ccctgctgaa ctgctggcgt ccctgattca gaccgctgaa
     6361 caggcattgt ggaaacgtga atgggccgcc cgggatcatg gcctcgccgt cccggaatgc
     6421 gtcacccgcc gtcaggcggt gattaatcag gcccgcaccc tgctgaaaaa caatagaaac
     6481 gaaaacgact gatgttatcg ccgcctccgg gcggcgattc agggagaacg attaatcatg
     6541 aacgaaaaca caacactgaa cgcactgatt tgtcgtcacg cccgcaacct gctgctggcg
     6601 cagggctggc cggaagagac ggatgttgac cagcggaacc cgaagtatcc gggctggatc
     6661 agcatttatg ttctgctgga tgcgccccgg ctggcgatgt tactcatcaa ccgtcacggc
     6721 ggcgtactgc cgccgctcct ggcctccgcc attcagaaac tgaccggaac cggggcggaa
     6781 ctggtactgt ctggcagtca gtggcagtcg ctgccggtac ttccggcaga cggaacacag
     6841 gtgtttttcc cgtatgccgg agaatggctg actgaagacg aaatcagggc agttcttgat
     6901 gcagtgcgcg atgcggtacg cagtgtcagt tgccgggtgg cagaagatgc gcagcgtatc
     6961 agggcggcgc tgaccacaac cggtcagacg ttgctgaccc gccagacgcg ccgctttcgc
     7021 ctggttgtga aggaaagcga tcacccctgc tggctcgatg aagacgacga aaacctgccc
     7081 gtggtgctcg atgccatcct gaaccgggga gcacgttttt cggcggtgga aatgtatctg
     7141 gtcagtgagt gtgtggagca tatcctgtcc agtgggctgg cctgcgatgt gctgcgtata
     7201 ccggatgaac cgccccgccg gtggtttgac cgtggtgttc tgcgggaggt ggtccgggaa
     7261 gcccgggccg aaatccgcag catggcggat gccctggcaa aaatcagggg ctgatgatgg
     7321 cggggaaaat ccccgccttt ttcctgccgg tcccggacag agcacaaagg acgtcgccgc
     7381 cggctccgct ttacccggcc atgcgggcat ggccttatgg gttttcagtt atgtggcctc
     7441 agcgccgtgt gcgggctgtg ccgtgcctcc atcttagccg ggctggcgtt gatgcaaggg
     7501 tacgcttcgc cgctgcggtc acccggtccc tccttcccgt ctgccgtgat tttccggtcg
     7561 ttcgcccgct caggtttcgc ggcctcaccc tgcaactccc gccagccgtg tgcgtcaggc
     7621 tgcggcttcc cttgcgcccc tgcatccccg cctgttcgcc cggcttttat ggaggcacgg
     7681 caccgcccgg tgtcgcggaa tatgtgaaca acggaggaaa acatcatgca atatgcgaaa
     7741 cctgtcactc tgaacgttga agagtgcgac cgtctctcct ttctgccgta tctgtttggc
     7801 aacgattttc tgtatgccga ggcgtatgtg tacgcgctgg cgcaaaaaat gatgccggaa
     7861 tatcagggcg gcttctggca cttcatccgc ctgccggacg gtggcggtta catgatgccg
     7921 gacggtgacc gtttccacat ggtgaacggt gcaaactggt ttgaccgtac cgtgagtgct
     7981 gatgccgcag gcatcatcct tacctccctt gtgattaacc gccagttgtg gctgtaccac
     8041 gacagcgggg atgcaggact gacccagctt taccggatgc gcgatgcgca gttgtggcgt
     8101 cacatcgaat ttcaccctga atgcaacgcg atttacgcgg ctctggactg attaacggac
     8161 ggggcggcaa tgccgcccct gtaaaggaga cgagaacatg tactgtactg ttaaagaaat
     8221 tatccgcgaa gtgctggata cagacgtgcc ggacagtgaa tgcgtttttg ccgtggtgct
     8281 gacccgtggg gatgtgcgtc acatagccca ggactggagt ctgacagacg atgagctgga
     8341 aactgtcatg cagcggctgg acgatgcctt tgagcatggt gcggatgtca gcgttgttca
     8401 cgacgttgtt cgtgaactga tggaagaaaa gcgcgccagc cgtcaggtga cagtcccggc
     8461 ggtgatgctg gaaaaagtgt tggcgctggc aggcagtgaa atgaagcgcc tgtatgccgt
     8521 cgggagtgag aacgggggcg acggtgatgc gttcgtcagg gaagaacgcg aagcaatgga
     8581 cgttgtgtta caggcgctgg acggggagca catgtcatga atatcagcac agaaacccgc
     8641 gaaattctgc gcaattacaa agccgtgatt aatgcgcggc gtcgtgaaat ggggcagaaa
     8701 ccgctcacca ctgcgcagat tgttgatgaa atctgcgatt ttgtggcgaa tcagcaggcg
     8761 gttttcctcg gtggtcacta tatccttcag ggcagcagaa acaggtgata cagggcaggc
     8821 ggtggaggcc gcctgtcagc ccgcgcgaaa acccctggca ggctgacgcc tgccggaaat
     8881 gcggggtgca gcgcctgcgg cgctgccggc ccggctttcc ggtcagaagg tagtgtatgg
     8941 ctgtatgcag ggaacaggtg tgatattccg tgacactgtc accgtttctg gctgtctttt
     9001 attgcgccca cttcggctcc cggctgcctg gcggcatccg gtcgtcaggc ccgactccga
     9061 caggcagctg ctggcgcacc cgccagtctc cgccgaaccc gctggcgcgg tttcgggctg
     9121 ccgctacggt ccgatgtctg aaaaaccagg cttgcagcga ccgttccggt cgcgccgccc
     9181 ctgtcgcgcc ggaaacggcg ctccctgcac acctgacggt gtgtaaggcc actgcgcctg
     9241 atggcgcagt caacccttcc gccgccaccc ctgcggcctg tttcccgctg tggactttcc
     9301 gggggctgga gtaaccacat cccgtttcag cagtccaggg gaagatatac cgtccacaaa
     9361 atttttagcc ataaacccgg caaaaaattt tgcgtccgcc cccggacagc tgccccgtga
     9421 cgtggtgacg gacgccccat cggaaaggtc cactgcgggg agaaaccccg aagaagtgga
     9481 cggcagaagg ggctttcgcc cctccgggga gcaaccggtc ggcccggcga cctgattcag
     9541 cagaaggaga cgttttcatg acagtcagca gcacgatttc cgttttttgc cgcgacgggg
     9601 tgttccgtac cgtttactgc cacctgcacg gggagccgac ctggaacggt cgcatcctgc
     9661 atacccacta tgccaccggt cagcaggccg aagccctggt tgaacacggt gatatccgtt
     9721 gcctcggtcc ccgttgcgac aaacccgccg ggcatacgct tcagaacccg gtggacggtg
     9781 tgacggctta ttacggacgt gacagtggtt tccggatgga cagtgaggcg cgtgagtacc
     9841 gttctttcag ggaggctatt gccactgaaa gcactgaaga ggtgcgcttc cattatgtgt
     9901 tcatcgacgg ctactggaag gtgatgtacc gcacgccgga aggctggaag atgaaagcgc
     9961 tcgcgctggc actgcgtcgc tgtccgaaat gaaaaaaggc agggcgataa accctgcctc
    10021 tctccgccgt tacttccccg ccaggaagag cggcagtcat caacaacata aggagaaata
    10081 tttgtgagac catcaattat cttcgcaacc gccgagtatg taaagcgtct gcgtgaagag
    10141 tgcctgcggg agaataaacc cctgcaccgt catacccgct tcagacgtca ggagctggca
    10201 caggatgaga ttaacccgga cgtcctggcg atgagcggtc atatcgccag acgctgcagt
    10261 gagcagaagc gggtacgtat ccccgccatg aaagtcagcg aatggggcca cctgctccgc
    10321 gcgcttgaaa ttgagcgggt ctgccactga ttaacccttg ccgtctgcct gtgcaggcgg
    10381 caggatacgt tcagacatct caccaggagg aaccgtgagt aagaagaaaa acaccacaac
    10441 gcccacgccg catgatgccg cgttccggtc gttcctggcg aatcccgacg tcgccagaga
    10501 ttttctggaa ctgcatcttc cggcggagta ccggcagttg tgcgacctgt ccacgctgaa
    10561 gctggaaccc gccacctttg ttgagccgga cctgcatcag tacgccagcg atatcctctg
    10621 gagtgtgaaa accaccgggg gtgaagacgg ctatgtttat acgctcatcg agcaccagag
    10681 caccgaaaat ctgtacatgc ctttccgcat gttacgttac agtgtggcgg cgatgcagag
    10741 acatctggag cagcacaaaa cgttgccact ggtcattccg gtgctgttct atcacggtga
    10801 gcgcagcccg tacccgtaca gcatgaactg gctggactgt tttgagaatc cggcgcttgc
    10861 ggctaaaata tacacaaagc cgtttccgct ggttgatatc actgtcgttg atgacaatga
    10921 aatcatgaac catcgccgga tggccgcact gacgctgctg atgaagcata tccgccatcg
    10981 tgacatgatg gagctgctgg acaaactccc gcaggtcatg gtggaaattt cagacgagca
    11041 ggtgcgtgtt ctgattcatt acatcgttaa cgcaggggac tctgtatcac cggaatttat
    11101 gcgggcgctg gctgagcgtc tgccgcagca tgaggataaa ctgatgacta tcgcagaacg
    11161 tcttgagcaa aaaggtcgcc aggaaggagc gcttgaaaaa gccctggcta ttgcgtgcca
    11221 gcttcagaaa atggggatga cgccggagca gattaagcag gctaccggac tttccgatga
    11281 cgaactgaag aaaatcactc actgatgaga accacaaagc ccggcaaaat gccgggcttt
    11341 tttgtcagca ggagcagagc aaacagcatg acgattgcag aacgtcttat acagaaaggt
    11401 ttcgacgagg gttttaaaca aggggctctt gaagtggcgc gggaaatagc ctgccggctt
    11461 cgggatatag gctggccgcc ggaacggatt caggaggtca cgggactttc cggtgaagaa
    11521 ctgaaaaagc tgtttcctga tgagcagtag cctggcattc agccaggctg tgaatcacgc
    11581 tcacacattc cctgccgttt cccccagttc attttccagt cgcctgatga tatctgcctt
    11641 acgcatcccc tgcatgtgag gcagcaggtc acggactgaa agtgcctggg tggtcctcca
    11701 gacggtgacg tccacctgtt ccgcattttc ccaggcgtgc catgttctct gcgtcacgcc
    11761 aaattctctg gccgcttttt cgcgtgacca gagcatgctt tttcgccaga ttcgcagttc
    11821 ccagccggtc ataaaacacc tttgaaaaag tgaaaaaact tcactttatt ttatcatcac
    11881 aattgccaaa tcagcgcgat aaataagtgt aaaaaaatga agaaatttca ctttttttgc
    11941 ttgtaaatgt cgtgcggttt tctctgtttt cacctgtgcg ggagtggtgt gccagtgacc
    12001 accacatgcc attgttgtgc cgtcgttttt cttcagtcgc gccgtatgcg gcgctccctg
    12061 cacaccttac ggtgtgtaag gccactgcgc ctgatggcgc agctgtctct gcctgtctgc
    12121 cgccgcactg cggcctgttc tccgcagacg gactttccgg gggtacatac atgaccacat
    12181 cccgtttcag tcgtccaggg gagagatgta ccagccacaa aagtttttgc cgcaggcgtc
    12241 aaaaactttt gcgtctgccc ccggacaact gccccgtgac gtggtgagcg tcccccgtcg
    12301 gaaaggtcca ctgcggggaa atccccgaag acccggacgg cagaaggagc ttcgctcctc
    12361 cgggaaatga cctggctgac ccggcagtcg tgacgatgag gagacaatgt gatgaatcag
    12421 actttaccca ctgctgacct gaatactgcc ggcacgacag atgttattcc gtctgtggct
    12481 atcgaccgca tcatcgcgca gcgtaacgaa ggtattgcac tgttcatgca ggcgatggaa
    12541 tgcctggcga cagcgcgcaa gattctgctc gatgcgtcag gtgatatttt tctttacggg
    12601 tttgaagact gcgtgactga ttccgttcgg tgcatggata aaccggaaga agcgaaaaag
    12661 aatatcaccc gtcttgccga ccgtaaaatc tgggaccgcc tgatgacgga tacggggatg
    12721 tacaccttca tgagttcatg tcagcgtgat gagtggaaca gccagttgat gagcgacacc
    12781 tgtcctgaaa tcaccctgga caatgtactg gcaacttttc gccatctgaa tgccagtaag
    12841 atgcagacat ttgaacaggg actgattgat gtctaccgga aattgtcatg ggattacaga
    12901 accaataatc cctgccgtct gggtaagaaa atcattattg aaaacctgct gtaccgctgg
    12961 agcaacgggc gtgtgacgct ggactgcagc ggacgggagg cactggatga cctggtacgt
    13021 ccgttttatc tgctggaggg gcgcaacgtt cctgacttca ggaacagcat cggggcgcag
    13081 tatggtgaat ttctcgggaa cggcgacaat gtcggtaagc tgttagaagg ggaatatttt
    13141 acggtgcgtg gctaccagaa agggaccgta cacattgtct ttaagcgttc tgaccttgtt
    13201 gaaaaaatga atgatattat tgcacggcat tatccaggtg cattgccgcc acgagtctga
    13261 ttaaacagaa aagccctggt atttatgccg gggctttttt ttctgtaatc atcataatct
    13321 cagaacacga tgtgacattg tcacatcaat tgttgttcat atcgatgcca tctttcctgc
    13381 ctgaatctgt ctttttaggt gctgttttag aggaagaacg gcgggatggt tttttcgatt
    13441 ctgcttcccg aataatgtca cgaagtgcct tctctcctac ttcataccct gcatttttga
    13501 gcgtgtcacg aacatctgcc agtgtataac cctttgtcct tactaggaca ataatgtcat
    13561 cacgaatggc atcaagaaaa tcacgcaatg ttttccgttg agcggtgaga tccggcaatt
    13621 ccgataaagc agcttttgcc agctggatgt catcatctga gtaaaatttt ttagaagcca
    13681 ttggcacttt ctctccgttt catgttgaag tctgatttta tcatcaaaaa ctgactcctg
    13741 gcagggctgc gccctgcctc tgccgtctgc ataagactat gatgcacaaa aataacaggc
    13801 tataatggcc tgaaaaacgg gacaggatgt gcaattgtaa taccgtcaca cgcgacgcta
    13861 ttacaattgc catctggtca gggcttcgcc ccgacacccc gtaaggagcc tgaagtgagt
    13921 gatagtgcgg taagaaagaa aagtgaggta cggcaaaaga cagttgtcag gacactcaga
    13981 ttctctcctg ttgaggatga aaccatcagg aaaaaagctg aagattccgg acttactgta
    14041 tctgcttaca tccgaaatgc agcactgaat aagcgaatta actcccgtac agatgatgct
    14101 tttctgaagg agttaatgag actgggaagg atgcagaagc atctgtttgt tcagggaaaa
    14161 agaaccggtg ataaggaata cgcagaagtg cttgttgcca taaccgaact tacaaataca
    14221 ttgcgtaaac agttaatgga aggttgagga ttctcctgtg aatgcagtca ttccgaaaaa
    14281 gagaagggac ggcaagtctt cgttcgaaga cctagtatcc tacgtctctg tccgggatga
    14341 catgaccgat gaggaactgg atttatcttc ttcttcgcag gctgaacaac cgcaccgaag
    14401 ccgtttcagt cgccttgttg attatgcgac acgtcttcga aatgagtcat tcgtggcgct
    14461 ggttgatgtc atgaaggacg gctgcgaatg ggtcaacttt tatggtgtca cctgctttca
    14521 taactgtact tctcttgaaa ctgccgctgc agatatggag tacattgccc ggcaggcaca
    14581 ctatgcaaaa gatgacactg atcctgtttt tcactacatc ctttcctggc agtcacatga
    14641 aagcccgcgt ccggaacaga tttatgacag tgtacgtcat acgctgaaat cactcggcct
    14701 tgccgaccat cagtatgtct ctgccgtgca tactgataca gataatctgc acgtccatgt
    14761 ggctgttaac cgggtacacc ctgaaacggg ttatctgaac cggttatcct ggagtcagga
    14821 aaaactcagt cgtgcctgcc gtgagcttga actgaagcat ggttttgctc cggataacgg
    14881 ttgctgggtc catgcaccgg gaaatcgtat cgttcgcaaa accgccgttg aacgtgatcg
    14941 ccagaacgcg tggacacgcg gaaaaaaaca aacttttcgc gagtatattg cgcagacagc
    15001 ggtagctggt ttacgcagtg aacctgtaca tgactggtta tccctgcatc gtcgtcttgc
    15061 ggaagatggt ctgtacctgt ctcagatgga cggaaaattt ctggtgatgg atggctggga
    15121 tcgcaacagg gaaggtgtac agcttgactc gtttggccct tcctggtgtg cagaaaaact
    15181 catgaaaaaa atgggtgact acacgccagt accaaaagac attttcagcc aggtggaagc
    15241 accaggacgc tataatccgg acttcattgc agctgatgtt cgcccggaaa aaatcgctga
    15301 aacagaaagt ctgcagcagt atgcctgtcg tcatcttgga gaacgtctgc cggaaatggc
    15361 acgggaagga cggctggaga actgccaggc tattcaccgc acactggctg aagcgggatt
    15421 atggatgcgt gttcagcatg gtcacctggt tatctgtgac ggttatgatc ataaccagac
    15481 tcccgttcgt gctgacagcg tatggtcact gctgacgctc gacaatgtga atcaactcga
    15541 tggtggctgg cagcctgtac caacagatat ttttctccag gttacgccaa cagaacgctt
    15601 ccgtggtcgt cgtatggaga gctgtcctgc gaccgataaa gaatggcacc gtatgcgtac
    15661 aggtacgggg ccgcaggggg ctatcaaacg cgaactgttt tctgacaaag aaagtctgtg
    15721 gggatacagc atcagccatt gtagccctca gattgaagaa atgatcacac agggtgagtt
    15781 tacctggcag cgttgtcatg agttgtttgc acaacaggga ctgatgctac agaaacaaca
    15841 tcacgggctt gttgtcgttg atgcctttaa tcatgagcaa acgccggtga aggccagcag
    15901 cattcatcct gatctgactc tggggcgtgc agagccacag gccggacctt ttgtgagtgc
    15961 cccagcagat ttgtttgaca gggtgcaacc tgaaagtcgc tataacccgg agctggctgt
    16021 cagtgacagg tacggggtca gcagtaaacg tgacccaatg ctgcgccggc agcgacgtga
    16081 agccagagct gaggcccgtg ctgacctgcg tgcccgctat ctggcatggc gtgaacaatg
    16141 gcgtaaacca gatctgcgtt atggggaacg ttgtcgggaa attcatcagg catgccgtct
    16201 gcggaagtca cacattcgtg cgcagtacga tgatccggca ttacgtaagc tgcattatca
    16261 cattgcagaa gttcagcgaa tgcaggcatt gatcaggctg aaagaagaca tcagggatga
    16321 gcggcagaaa cttattgctg acgggaagtg gtatccaccc tcttaccgtc agtgggtcga
    16381 aattcaggct gctcagggag acagggctgc tgtatcgcag ctgagaggct gggattatcg
    16441 cgatcgccgt aaagataagt cacgcacaac gacaacagac cgctgtgtcg tgctttgtga
    16501 accgggcgga acgccggtat acggtaatac cggtgatctc gaggcccgtt tgcagaagaa
    16561 cgggagtgtt cgtttccgtg accgtcggac gggagagttt gtctgtacgg attatggtga
    16621 cagggttgtt ttccgtaatc accatgaccg caatgcgctg gcagataaac tggatttgat
    16681 tgcaccggta ttattcgggc gtgatccacg catgggtttt gaaccagaag gaaacgacaa
    16741 acaatttaac caggtctttg ctgaaatggt tgcctggcat aatgtgacag gccgtaccgg
    16801 tcacgaagac tacaggatta cgcgaccgga cgtggatcat caccgtgaag gaagtgaacg
    16861 ctactaccgc gattatatcg cggcgaacag caacgatgac gcatcactgc ctccgccgga
    16921 gcaggataaa cgctgggaac cgccttctcc aggttaaaaa aaaggccgcg ccagaatccg
    16981 gcgcggccac tgttcagtac attccgtccc agtccggacg gttggttttt ttatgatgaa
    17041 acccgtttac aggacgccct gtcgggtggt tattgtcctg tggaagcgtt aacaggttta
    17101 tacctgctct ttctgctctc actgatatct gattttcctc ttttgtttca ggtagttctg
    17161 gtctgttcag cgtgatatag cgaattcgtc gctcttcccg tgtggtcgtt ttaagcgtat
    17221 tcaccgctgt ttcccacaag gcactttcac gtgcgttgat atctgtgtcg tactcttcca
    17281 gcattgccat agccgcctga cgtcctgcaa tgcgttgctg gaatgtatcc acgatcgtgt
    17341 gcggctctgt gcgaattttt cgtctttttc tggagggttt tgcagtcagg acgccaatga
    17401 tggcactgac attttccggt gacggtattc gacgttgagt agtacgggga acaagacggc
    17461 ggagacggtc caggcgtggc gcatccacgc tgatatacct gtttataaca acgggatcgc
    17521 tgctgcagct tttctcatcg tcagggatga agaatgatgc tgatggtacg gtttctcccc
    17581 ggaaaataga aaagaactcg ccaggatgaa gttcaatcaa ctcctgaaca tttatcttgc
    17641 tttccatcag gatgctgatt tgtgggctgt caatccatga ttcaccaaat atgccgtcct
    17701 gacgctgcag gttgttcatt ctcgccctgg cttcttcacc ggcggcgctt ttcagcgttc
    17761 tggccgtact accctcgctg acaatcctgc cggcaaattt tgtccccgtg ttctgcatca
    17821 gtgttgcagt attcgtggct gttgtctgtc cttctatacg ttcctggtcc tgtgccatca
    17881 aaatcagagc aaaatccagt gaacgcacct gagtggcttc cacagcaata cggtccgtat
    17941 agtatgcccc cacttcatca aggaaacaca gataagggaa acgcccctta tattttttga
    18001 cttccagtgc atcggaatca gttccttcga ggcggtagcc caaatcacgg gcgagaatca
    18061 tacttttctg ggtgataaac attcgtccaa gagcagacgt tgtatgcgct gatgtatcca
    18121 gagcggggat cattaccatc agaatcctgt cgctgtgaat gctgtcacgg atatcgatat
    18181 caccggaatc ttcagcaaaa atatccccga atgcttccgt gaatgtgctg aaagtttcag
    18241 aaaactgccc ggagagataa gcatgttgtt ttctgggttc ttccgtccag gctgatggtg
    18301 tcctgaccag cgacaggtcg aaaccgggaa cgtcctgaag gtagttacgt aacggtgcga
    18361 tagcttcttc cggccactga tcatcgaggc cacgacagta aagtttggcc atcccttcga
    18421 gagtcatatg ttcacggagc atctgcagcg acattgtttt gccttcgcgc acacaccaga
    18481 atttagtacc aaataccagt gccttattca tggcaatggc ccgggactgc cactcgcccc
    18541 cctggacgtt ctggggcagc attgactgca ttgtctccgc tgtgaatgcc tcagtgctgt
    18601 aacagaaggg attccaggta tttgactgag gacgcgtttt ttcaccactc agaatgattt
    18661 cactgcggga ttttcctccg ttcatgaagt taatgacttc cacatcgtct tcacgtccaa
    18721 agcgtctggc cagataccag atcgttcttg ccgtatcatt ctgtgccttg ccatcgacaa
    18781 gggtgaatcc tcttgcccag caaagtgggt taatcgccca ggcaaagatg gtttccgttt
    18841 taccgccacc ggtggtggcg aaaaacatta tatggcgggt cagatcgtcc atactcagcc
    18901 ataactccct gccaatatcc ctgacgcgtt gataaccaac atagaatatg ccgctggccg
    18961 gtgattccag aatgacctca tactggaaca gggttggcca gaagctgaaa agacttcttt
    19021 taatcattct gtcctgtgac gggtcagcac attccagcgt catgggcata cgaagagggc
    19081 agcgccatcg ctgcagacta aataccagca ttgttatcag tgtgaacaac agacatgccg
    19141 gtaatgcaag tggccagata aaactggcca caagacttgc caccaggcaa aagccataga
    19201 tattgagact ctggagtgca ttccagacag gatttcccca ggctgtacgg tgtaaaagct
    19261 cggggttaac gcgatgttca ctcatggcca gactcgctgt cggtggtttt caggaataaa
    19321 ttaagcgtgc cagtttcctg cagaattcct gtaggcagat gccagccggt gtcggacagc
    19381 acccatgggg taccggcaaa tccgaactta cggaaggcga cattgttaac atcaatcgca
    19441 gctctggctg ctttcaggca tgtttcatct gcaggcgttg tttcttctga tggcgtcatc
    19501 attccgttat ctgcggaata cagtcggtgc cagccggcag cacgtttctg agcatctttt
    19561 tcacacagca gtggtgccac acggtcggtg gattcctctc caccaataac tgaaaccggg
    19621 tagataacga cattgaagtc gctggccagg cgttttaagg ccggctccag aagtctgcag
    19681 ttaggacagg caggatcgag aaatgcgaac agggttcgtt catgaccgga tgacaggttg
    19741 atggtaaaca tgccccgatc ggcgttccgc ttcagaacag tagccagatt atgtcgcgca
    19801 gctgcggctt ccggattttt gcttgtgatt gttttctccg ggacctggac gtcttttttc
    19861 gggggaaccg ggagttgaat gccttcattt aatggcacag tgtgattcag gtgagggggt
    19921 agcagtcgtg gctcacctgc tgtctgtact gcttcaggcg ttgtatcatc ccagaataca
    19981 cgatcaacat agcggaacag agagaagcca atcgttgcca ttacgaacag cacaacgctg
    20041 gcgattatgc cacggaacaa aggtgttctg ctgaagcggc gttccatcag atattcaagg
    20101 cgcaggcagg aatcaatagc tgtaagtatc tggtcagcgt tatgctcctg tttatcaatc
    20161 aggacggttt ctctgttacg ggtgcccatg gcgtcaaagg ctatatgaat cagttgatta
    20221 tttcgtgtta ccagccctga cagggcatcc cgtggaagga cggcaataat ttctgtcctg
    20281 ccatccggcc agattgcctg cacggaaata tgttttgcaa tgcgtgttgc aaagtactcg
    20341 attgtcattt tcagttctcc ggataatcag aattcgttgt ctgtagcagg tgtgcggggc
    20401 aggatgtcgt cggtgaggga ccagttggtc aggatgcggc gtttccggtc gcgatctggt
    20461 tgtggttcat cccagataag accgagactg agcatatccc gccgtaagcc ttccaccgct
    20521 tcatcaacac agggttcagg gcagggaaga ccaaaacgca tggcttcggc ctctgcacgt
    20581 gcaacggcca ccacgccagc gccttcaata aatcctttcg tggtattggc gcggtgtaat
    20641 gcataaaaca gcgtcctgtc cacgcctttc agccaaatcc agttgggtgg cgtaagccgc
    20701 aggtcatgtg catacagcca gaccagccca ctgcggacgt agcgatgttg tttcagccat
    20761 tgctgtgctc cgttgctttt aatcactctc tggaaagctg atttggccag tgaataaaca
    20821 ggggtacaga atttcccact gtcccgtttg cttttaattc tgcatgagag gttcagtgta
    20881 tccatgagct ttaatgctgc cttacgatca tccaggaaat actgaagacc aaatatggca
    20941 aagagcgctt tttcatgtgg tgccatgtct ttccaggacg ttaaaggttt acctaactgg
    21001 gccatgaaac atcgtcttgc agctgcaaca tcgagctgca tattggtgat gagagtatgc
    21061 tgctcgacaa atgcttccgg ggtgagagca acccgccgtt caggtcgttt gccaccatga
    21121 aagagttttt ccggatcacc atctgccagt acaggggcga tagcagggga caacgaagca
    21181 aagatgtgtg gcaggcgggt aatatctact ggtctgcggg tgaaacgact ttgtcccgga
    21241 tgttgatacc actcccacaa acaccagagt gtcacaggta agaggtacat ccacagaata
    21301 cctatggtct gctccatgac gttaacccac tggctatagc ttatgttggc ggcgttgtta
    21361 ccggtcatgg caagcaggtt gtatcgtggt gctgcatagt tatgaaatgg tccccagtca
    21421 accagtcccc ataaggtatg aagaatcagg cagctggcgt aaaccacttc cggcaagaat
    21481 aaccatataa cgaatagcag caggataaga aggacaccaa cagcgcccca tatctgcata
    21541 ggatcttctg cgacaggctg tcgattgtaa gacatgacaa agtctccatt tctggaattt
    21601 aaatcaaacg gaaaggttaa aaatttattt gcaggcgata agagctaacc agaggaatta
    21661 gccgtgtgat aaaaggattg ttgcagatac gaaagtcgtt agaatagctg cgggcgcttg
    21721 aggctgtatg ccgaaagcgt tttgtggacg gtatacagca gaaagcccct ggagattttt
    21781 ttatcaatca accaagggct ctactgtaat gcttcgacaa cattatagta gcccgataac
    21841 cgccgtaagg caatggaggg gctatgatgc cacagcgaac gtttttaatg atgttaatcg
    21901 tcatctgtgt gacgattctg tgttttgtct ggatggtgag ggattcgctt tgcgtactcc
    21961 ggctccagca gggaaacaca gtgcttgtgg caacgttagc ctacgaagtt aaacgttaac
    22021 gggcaacacg gcggcaggtt ttctgccgcc gctttgttgc gaaggctgac ctcattgcgc
    22081 cctttcttct tcaatgaagc gtatccggta ttgtttcatt ttccctgaag ccgattagct
    22141 ccagccgaat gcctttgcca tcgggggcca cgttctgcat ttcgcagata ccacgtcggg
    22201 tcatgagtgt gaaggctgtc attgccagca tggctaacgc gttttcctcg cttccgctgc
    22261 gtttctcgag agcatcggca atttcttcta tgctccccac ctgaaagcaa gagtcgaatt
    22321 ctttactcca cttttctgac atcggatttt ctctctgtat ttatgggtag ttatcgaact
    22381 gaatgtgcca gttttttcag gagacggcaa agttgagatg gttggttctg attcaaagca
    22441 tgagtttccc cggtgtgacg ggcatgtcgg gcggattttg agaaacaaca gatgtaaaaa
    22501 aaccgactgg tgaggtcggt ttttttactg ttccgttaca gagtgggtgg ctctggtcgg
    22561 aaaagaactg tctataactg acctgtagat agtacgtctg gaattatatt tctgcaagta
    22621 ctggataagt gtatactcgg catgtcagct ctgaggcatg ttctgtccgg catattgttc
    22681 agcgggaaga acaggttaca agtcctggcc aaaacatcag gtggtgctgg ttttggtgct
    22741 ctggtcggag aagaactgtc tataactgac actatagaaa ccgttgtgac aggcagtgta
    22801 agagcgaaca acggggttct ttctccagtg atggagaagc ggatgattaa tctgttaatc
    22861 atcgttctca gggcagtagt tgccgttgca aacgcgctga ttgctgttct ggaactgatc
    22921 cgggaactta tcgactgata agcctggaaa cgtaactaag ggcagggagt taccgcttcc
    22981 tgccctttaa caaaaagaga tctcacgtta aaaatttgta cccttcttta acttccagtg
    23041 cctgctcctt tgactcaaac atcagtcttg ttttcccggg aacgccccat gaaatgtatt
    23101 caacatctat ccaccatttt ccatattctt tatatggccc ggatattact tttttgacaa
    23161 aggcatctac cagattcatt gttttttcct ttttgtgttg aaaataatta atgccagagc
    23221 acagcgaaaa taatgaatgc gctggctgtg ataattgcga ttttctccca tatgatattt
    23281 ttctcatggg ggtttctacg gagattattt cttcgcattg agataccggt attcatgttt
    23341 tatcttggac ttttgttttg ttgtctgtct ggcaggctgt tctgcgcccg tttgtgacaa
    23401 ggttagctta tggggttaaa tggtcacgga catcatgcgg acggttccct gtcacatgcc
    23461 atctggtttt ttcattcaaa ttgagagata ttgctaagtt gagtcgcttg cgatagtgga
    23521 ctcctgtttg taagtgagtg ccattgttta tttcatgaag aggattgatt atgagcatgc
    23581 ctcctgcgat agctaatact ttcttgtttg aaatgatgaa gagtaaatca aaagacgcta
    23641 cacttgctgc gatctatgca ttaggtgaag gccggtgtca ggcggagaac attactcgtg
    23701 aattacatag attgagtcaa agtgatgata tggaaataaa aattgctgcg attaaagcac
    23761 ttggtcgaat ttatcggtaa cggcataagt tatctgttgt aatgaacaaa agccggctca
    23821 tttgaaccgg ctgttatgga gggagcttat cttaaaatac ttcggctaca ggcatgcctg
    23881 caatctgctc ccactcctta cgcttctcct gaatcaggcg aggaaagtcg acagggtaat
    23941 catatcctcg acttgccata gcgtgcatgg tgtttgatgc ggtttctgga tgagtacacc
    24001 atgatggaat aggaaccatt ggcaggttga tgtatttcgt atgaatttcc agtgttttgt
    24061 cgtctgttac agtacgagtg aaattatgaa tgtcaaaacc gataacatcc ataatgttgc
    24121 ctggatagat acggacatac aacattgtgc catttttaag gtcaataccg aaataagctc
    24181 cctgagttaa agataatcct tcatacccct tctctggaga gtagaagcct gactctttta
    24241 ttgtttgtac aacttttctt atcttttctc tccttgtttt tgcacgtgaa tacatccata
    24301 tacctggcgc aagtaataag aaccaactac caaaagtgac aacataatct tcaatgttgt
    24361 caccataata acgagaacta aataacatca gtgtagatgt cagtgcgcaa aataaaaaga
    24421 aaggtattcc taaaaagaag taagccttcc ttaatacata atataacaac caccaagttg
    24481 gaaatctgtc aggtgtctct tttttatctt tcatacaatg cctcccctcg tactatctat
    24541 tattttaatg ccgatgggga ggttgtgcaa tatttatatt atccttttat tccgtccttg
    24601 tcgtcacttc ctttatcttt aagacgatct gttttcattc caggagtatg tcggatattg
    24661 cgacttcctc caataaatat ttctttaaca gaatttgcca tgctgccaag agtgttggct
    24721 ccctcacgcc caccaaggaa attaataaca tgatcaggta ggtaggcctg aagggcaaat
    24781 atggcagcta cagaggttgt acaaattctg gcgtatatca gcaagaaacc agcaagactg
    24841 aaaataccgg tcagcgaatt gaactgtacg ttaaccaggg cagcgccaaa taatgcattc
    24901 agtattgtgc ctacggcgat gatggcaaca gaagcaaaga aaaaaccaaa gaccatgatg
    24961 ggcggcctta tcatactatc aattaagtat atataaccgt aggctgcccg acttcctcta
    25021 tcttctgatg ttccgagatg tgttgcagcc cacagaggtc cggcggtaca tcctattaat
    25081 acactgacta tccagttccc aatgcctgtc atccagaaaa tgaatggtat aaatggtagg
    25141 taaattgata aggaaaaacc cgcacaaaac aacaggaaca acaagaaata tattggggga
    25201 gctgcagcat ccagcagagc aactaatcct ttaggtaatc cagagaataa attggcaacc
    25261 tttccgaaaa tagagttatc acctactgtc gcgagaactc taactgttgt atatccactc
    25321 cagagaatct ctgttgttcc taatgtataa tcaccaatat ttttcatctt tatcaatgga
    25381 ttgacctgat cgcttgttgt tcctgtgcca cttatctctg ttgctatcca gttggttcga
    25441 tagacaaaga agctcatcat ttcagataag gcagacttag gatcttgcgc attggccatt
    25501 cgttgctcat tggctgatgt aacagtgccc agaggtggtg tataggtggt gttctgtaat
    25561 tgagtcttgt aggcactcat gactgcactg aataagtcag tatcaccaac ttctcccagt
    25621 gatgacattg aagttacagc tggagctctg tcagccagct cggccagacg ctggttagca
    25681 gtggcaaagg tctgatacca tgcgccaagc gttacccacc cgtaggttgt gagataagat
    25741 ttaagagcct cttttctgga ttgctctccg ttatcagtgg gtagcgattt ttgtacagca
    25801 cgttcatact cgtcggctgc tctctgaatc cgtgtctcga tatccggcaa tgttccgttg
    25861 ccagaatttc ttttttccag aaatgttgtc acaaactcac tggcagcgtt gtccatgtcg
    25921 ctaatcatat tatccatggc tgcacgttgt gctgagataa caccgctgta ttcgttttta
    25981 ctgaagggat taaagaattt tccaatcgtc gattgatctg ttgttccgtt tccttctact
    26041 gacaggctgg ctgaaccaca gataccgctg ccattggata ccgtgacagt gtaattacca
    26101 ctggcggttt tagctgattc tgtcatcagt gaagtcgagg atttggccgt ctgattaaaa
    26161 tcatttagtc ctgcgttaac cgcatactta cacagttcca tttcaaaaat gccacgcgct
    26221 gctgtttgtg tggatgcctg aactggctgt acagtcattg aataaccatt tgcgatatta
    26281 tcagcggcaa gttgcaccat cacattggcg gacccaaccc ccattatcga tgcgccccat
    26341 aacataatga gctgagcgat actccagcca tttccggttg gaacgatcat cagaaaacct
    26401 gcaacgacac tcagtgtgcc gacgatatcg cgtccggtgc tgaatacctg tccctgatgc
    26461 cccgaacgca caacatgacg gataccaatg aacataaacc agacaacggc aagtacggcg
    26521 atgatactgt tgaacgcccc gaacatgctt ccgatcagag tgggggcgct ggttgacagt
    26581 ggattggtga ctacatcacc gaatatcgtg accagcgcct ggcgagacag atcgtcagga
    26641 ttggttgcgg cagacacaat atcctgatag gtcactgaag ccattgccgg cagtgaggat
    26701 atagcaaggc ctgcacagag tgcgcgaagt aaaattttca cagtaactcc ggaatagaat
    26761 tgcgtatggg caaaggccac acaggattta attaaggggg atggcgacat gccagtggta
    26821 ctggcatctg gacgtgatca gtattgttta ctggttacgg caattaacgt ggcattgcgc
    26881 cagccattgc ggtcgttcag aaaatcacgg aaagtcccct gctcgggttc gctgactttt
    26941 cgttcatgca actgccagag gcggtatgta acaatcagtg ctctggaagc acaaagagcg
    27001 acgcctgaca gtatcagcac aagagtgctg atggcgcgta ccagagtcgt aagaggtaat
    27061 gaagaggcgg caaagagcat accactgaga aacaaagcga tggccagcag actccagcaa
    27121 cagaatcgcc atactgttct gcgccggcgg aaacgacgat ccagatcgtc tactgacata
    27181 tggctggcct gcagagcttc ttcccacgtg aggtggagtt gcgccttttt attatttacc
    27241 tgacgaaatc tgtccagtct gcgtttttgt tgttccagca tatgctgtac agaattcgtt
    27301 atcaggcgag tctccatcag aggaacaaaa atgttaataa tcccccgggt ggctatgcgg
    27361 gttatggatg gtgatttttt gacgtttttc gtctggtctg aattttccgg taacttattc
    27421 attttttcag tcctgttctg acgtcagctt tcaacgtgcg tttgcctgat tgattgtttt
    27481 ctcaaggcta ccgagttgtt tttcataata ctggcgggcg gtcagagcca gttgctgtcc
    27541 agagatgaca ttaccctggc ggatctgttc tttcaggtcg ttcagttgcc agttcatttg
    27601 cgcagtgatt cttaccagtt cacggagcag gttatctccc tgcatttcct gtaaatccac
    27661 caggtatgct gtgttggcgt agcgccggcc agcctcaaaa gcttcaaact cacgggtact
    27721 catatatccc cgtgcttttg cttctggtga agccacctgg tcgaagtaag cggccgctga
    27781 gtctgactgc agtgtttcag ccagtgcttt acgtgtgctt tcattaggcg tgctgtcagc
    27841 aatcagcgtc agttggggat tcgacgcaga atcaatgatg ccgttgtatt cgttctggag
    27901 tccgacgtag gttcgcccac tctgggtatt cacttctcct ttacccagag cacgaccggc
    27961 agaggggcgg gaagtgtttt tcatgtaagc cgtcgctgca tctatttgct tctgatccca
    28021 tgtcagtgct gcaggtgtgt ccgctgtacc tgcgccgtgg taaatcgagc gtacctgact
    28081 gtctccacct ggtatttcgc ccacagaagg acaaacggcg gttcctccaa aacgtgcata
    28141 ttctgcttct gtacagtaac tggcgtgtat ggccgcgcca tcataagctt cccgcacggg
    28201 agagtttgct gcgctggcca gacggtcgcg gatgctacgg ttgctcacac cgccaccttt
    28261 actgagtttt gaggctgcgg atgctgaggc acttttgctt tctgttgcaa tccctgatgc
    28321 ggattcactg cagatggagt ctggcacggt gaagcttttc cgtgcctcct cgaggcgctg
    28381 agtttcatta ctgaaaatca tctgctgacg tgtcgcctgt ccgttctggg caatgacact
    28441 tgccagcttt tcagagttct gattgatagc ggtacccgtg gctgtctggg tactcagtat
    28501 gttgccaagg acatctttga tggcttccat tctggggatg acctgctctt caacaggccg
    28561 gctggcgacg acggtgactg aataagcatg agcctggcat gccgttccac tcagggtggc
    28621 tataagggct gcgagtaatg tttttctttg cattttattg tctcctgctc gggatggagc
    28681 acaaggtcag gaaggctgtc gtattttctg ttttcaggta gtggggccag tgggaaacac
    28741 tgaaatcgcc tggtgatata acctttcctg ttccggttat cgatgatgct gaaagaacga
    28801 cgtctgacgc gccgtaaggt ggaagggtca gtatccataa caggccagtc accggcactc
    28861 agtgccggcg ttccgggaac ggatggccat aaccaggtat catcggacat cagcgaagcc
    28921 agcggtgaag ggagtatcca ctggatgaca tgctgaacgt cgaatggtgc ttcatccggg
    28981 acagaaacct gccatattgc cgttgttatc agctcatctg tgctggacgg caacaacaac
    29041 cgcatgcctg taacctttcc ctgtgttctg gcatccgagg cgtatatcac tctgggattg
    29101 ggggccggat gtaacctgaa gacacgtgac ggcagagtga atgagaaact gtcacctttt
    29161 acctcaagtt ttttaagaaa tacctgccct ctggcaggtc tccggcagcg tggggtgata
    29221 tgaattgtac acatcagaga ttgtatccct gttttcggat gagctccgag gccagttctt
    29281 caataacatt gtcactgtta tgttggcccg cccggtgctc aattaatgac gttgcagaac
    29341 ctctggggaa gttttcggcc agaatcttac gtgcccgaac tgaacctaac ttatttgtca
    29401 gtgttttacg cagggcactg tcttttggcg atgagttcag cgcccatagc tccagtggac
    29461 cgacggtaaa tttgagtatt ctcgcaagcg tgccactttt taccctgaat actcccagaa
    29521 caggaacacc actaccatct ggtgccgggc cttccggcat tttcaggaag cgtttcagca
    29581 taaattcagg aacgttgaag ttatccctca gtacggggat atcttcgggt ttgtagcgca
    29641 gtaaccagag agtatttgct gacttaagaa cgctttccgg gtagtccctc agatactgcg
    29701 tactcagaac cgtgcggata gcaaacttgc gttgctcacg ctcctgggta tccagatttt
    29761 cccagatgaa gtcaatgccc cgggcgttat gcagttcatc ataaactttt gtcttaacct
    29821 cctgatcaag ttggttaata cgtttaagag cgatttcatg gtattcccgt ggaagctgct
    29881 ttaacacttc atccctgtat tgtggcaggg tgaagtcacc accggcgatc tgacctgcaa
    29941 gcaggtacat gatgccggtt ttaagcctgc ctgcaggcgt tttgtcaccg gcaacattat
    30001 tcagatcgac agcgatgacc cgggtttccg ggttgatcat aaaccgggta tacccggata
    30061 tcatggggta gtcgttatgt ccctgctcca gggcgcggcg gatgtagtcc agcagaagct
    30121 cgtttgaacc atcacgctga acagtaccga aaacatccct gagactggta tggcccagca
    30181 tcgaggacac ttcgggaagc tggggcattg cctgataatg tgcccgctga gccgccatga
    30241 tatacccggc attgtggagc atatcccgga cttcgaacca ggttgatctg gcccaccagg
    30301 ttgcatcatg tttttcatac agtcccgagt cctgcagtgc tgaatccacc agttcttccg
    30361 ttgaggcgcg ataaagtcgc ggattatttt caccgtactc tttaaaagcc agctcgataa
    30421 gctggttgat aatttgtcgg gtgtctccgg ggttacaggg ttgaccggta ccggtatcga
    30481 cacagagagc acacagcacc gatgacatga aattcttttc cggcgtaata ggctttttag
    30541 cgccgtacat cacgtcaaaa aggttacggg tgtattccgg atcattgctc agaataatac
    30601 cgacagcttc gtcttttctt tccggaggaa gactgtcgcg aataagctga accagtccct
    30661 gagcactgta tcccttatcg atataggcaa tgaagggcag gtttttctga gcagatgata
    30721 tctgtatttc cgacaatgta ttaatcagaa ctgatttacc cagtcccgga tcgcctggtg
    30781 cgagttctgt atgtttattt tgttgggatg acgccagacc aacctcaatt gcggaaccgt
    30841 cttctgtatg taacatcaga ttccctttgc ctcgccagac agagcctgcc ctgttaagcg
    30901 ggaaaagcga gatggcatgt gacaacgggg gatacagggg aaccgggccg gagcctccgg
    30961 atgccgcgag aatggtattc acccaggcgc gtcggggatc gccaaatgtt gtcgtggtgc
    31021 cacatacgcc ccagccttca atggctgatt tcagaatggc ctggtttcgt gtgcatattt
    31081 cacgtgtttt tccccacgtg gaggccatga tggtcattat gcagacaggt tctttttcat
    31141 ccgttgccgc cagggccata acggattcat acattggacg aacggcagaa atgaaactgc
    31201 tgtaagtcag aagcgttttc ttcagattaa gagccttcat cccgccgggc atcagatcca
    31261 ttctgatgcg ccaggggact gcgcgtggga cggcacggac cagttcgtta aacgtctgca
    31321 gattttgcgg aggtaaagta atcgatacca ttccgtgcca gagtccgccc gcctgaacca
    31381 ggttgccctg tgtggtcacc tgagtgttga ataactgcaa atgcaatgaa ggagcatgta
    31441 gtacggattc atcgtcagtc cagcgatatc ccgctggctg ggcatcttca ggcaggtggg
    31501 gttgccagtt acgcggtgtg ctgtgccgtt ctgtctgacg acgtatttca cgcccgacct
    31561 cgtgtatatc cagtagccgg agaatcaggc catcagatga atgtctcagt gcctgttcca
    31621 cattatcaag aaaagcttcg tggcggattt tcagtgcaga taaggtccac tgccaggggg
    31681 actgggcaaa gcgtgctttc ggtacccgtt cagccagtct tctgacaagc tcatcatggg
    31741 cggtacgatc gctgttgctg ataaggtcgg gtccactcca gatcgccagc cagcaacgct
    31801 cccggaccag ccatggggac agtgttgtga ctttctcatc gaccacatcc tgtaactgga
    31861 tacctgtatt agccagtgag cgcttttgtg gggcgaccat gtcttcgatt tcttctttcc
    31921 ccatgtcagg atcacgctca aaaacgaagc tgattttatg accgctgttt ttatatgccg
    31981 tattcaggct gtcactcatg ctggtgatca gcgattccag tgaacctggg ccagtctggt
    32041 cggatgcttc atccatttcc ctgaaagcac cgctgacttc aaagacagag agatattctc
    32101 cacgctttgt ggctgcaata tagggcgaat tcagccatgg gcgacgttca cgatcctgtc
    32161 tgtccagacc tattacagta cgtaaatcgc aatactcaac aaaactgctt gcgacagaga
    32221 aacgggatag cgtgttcaga cagtcttcga tagtgaagat taaacggttt atgttcatag
    32281 gtgagggaac tccggactta caggagggag gtttgtgcag tttgattttt ttaatgccac
    32341 cccgcgctgg agtggcatga agaaaaatca atcaacgtat tcttttttga tgaatgaatc
    32401 tggtgtcaga atcagggcaa tcccgttcat tttttcacgc aggggcgaac gaccggaagg
    32461 tggatagcga cgaaaaacgt cagcgaaatc ggccgggcgg aaactgcgga atttatcctc
    32521 aaccgccagt ctgttttgca tcagtgcaga gagcgcttcc cgtccccgcc ggacccggaa
    32581 cttatcatta ctgaaaactt cctgtaatga acgacggtta agggccacga ccgtggatgg
    32641 atgaaggtcg ggtagccaca ccataacgcc ctgtgctgcg gcataaccat tcagtgaatg
    32701 ccaggcgtgg cagaaagcac ataagctgta aggcgggccg tcttcatgaa acagttcctg
    32761 ctctttctct gacaacgact ctgtactggt gatttcagct ttctgtacat ggcggagtga
    32821 aagacgtctg gcagacagac gggcctgctc aagcgcccgt tcagcggctt ttactgccgt
    32881 gttgtacttt tccttttcgc cggttgcgac tggttcacca cgctttttta acttatccag
    32941 atgttctgtg gccttattaa gacgaactcg tgtgtcactg attgtgtcgg taagctgtct
    33001 gagtggccat gagtcatcca gtctgaaacg gtgcctgacg ttttgccggc agcagataca
    33061 tcgaccgggc gttgttaatt ccggttccgg taacagtggc tcctgtgtat gaaccacatc
    33121 aatttctgga aagggataca accacaagtc tttaatgctg tgttgcatga gcaggctccg
    33181 tactggtgta acgtttcacg gcttccagaa tgatgtttgc gatttcatcg gaacagcatt
    33241 ctgcaaaaat gaattcctga tcgtcccgac tgtcgtgggc aatcacattg ccgttttctg
    33301 ttcgtcgtaa cgttatgtgc tccgggctcc aggcagacat atgtgcctcc ttgtattgcc
    33361 gaacaggaag ggcaccggat aaatgccagt gccctggaca ggattagcca atggaaattg
    33421 aggaaatccc caactgtttc tggctgcgac tcatcatttc aggtccggcg gcaagtagcg
    33481 ccccaatgac gatagataga atgcaaccag ccagtcccat ccccccttgt ttcccaactt
    33541 ttttcagccc aatcaggccc ccgagaaacg caattacgcc gatgaactgc gcaatagtca
    33601 gtgatgatga ctgtgctgtt ttggcacctt gttcgacatt gcgaaccata tcggcaagat
    33661 ctccatcggc aagaacggct ttgggcagca acatcatggg aatgagcagc aggaacttag
    33721 tcagtttgct ccggcatgac attacaaaca gttgtgcgtg gataaatgcg gcgtacagac
    33781 gggccgctac gacacgaata cgtgacatag tttctcctta aatggatata tcagtggttt
    33841 atatgaaggt gaaaaaaatc agaagacgat tccaatgctt gccagcaggg cattgaggac
    33901 acgatcgata aagaccagca ggacgccggc aataaatttg acgtttccct tactgacgga
    33961 ttcactggca ctcagtgcgg tatgtccatc gagtccggcc ttgcgccaga tggatatccc
    34021 gttcattaca aatccaacac cggccagctt ggccagcgac agaactgcat ttgctgcgcc
    34081 agcagcagcg ccaaacgtct gaggttgtac atatgcaatc gggccaaatg agtcagccct
    34141 gaatcccatt tgtacgcctc cggcgttaat cattgccggg agtgatacga gcatgcagct
    34201 ggttaacatc cccgcaataa attttccggg agacattttt accggaccgg ctgaacgggc
    34261 gcgggcaaac agggtaatca tgctgatcac cccgatgaat tctccccagg taaacaggaa
    34321 atccattccg gcactgaaaa tactgctggc aaagttctca atggcggtca gggcatccat
    34381 gttcatcagc gtaacgtccc tgcactggtg atgatggtcc gggtagtcgg atcaatccgc
    34441 tggatcacca cattgcccag cgtctgacct tctctgacag cccaggtact accctgccag
    34501 cgaatccagg ccataccagt ttccatcgac atgattttca ttccgttaag tacagacgac
    34561 gtttttttct ttgctggatg cggtgtggaa gaggattcag tgacgctttt ctgtgtcatt
    34621 attccgttca ggcggctccg gatatcatcc agctgacggc gtgtttcctg tgcgtattct
    34681 tcatcctttt ttatagcttc gcccagcttg ttaatgctgt cgtgaagcat attcagtgtt
    34741 gcctgaattt tttcatcgcg gttatccagc tctgttttta catcccgggc aagctggctc
    34801 atatcttctc ctgtccggac agtcatagtc tcagtggact gaacggaagg tgatgattct
    34861 tgtgtggttt gccaggtctg tacgccagca tccgcatcat taaacggagg catgcctgtt
    34921 tccgatggcg ctctcaggaa cagataccat atggctgcga gcaacacgac tgcacatagc
    34981 ccccatgttg ctacagatat cccccagacg gaacgtttcc agaagggggc gggtttctct
    35041 tcgggttcac cgaagctgcc ggtttcatct atttctgttt caggtttcat tatggtgtct
    35101 ccggttgttc atcataccga cgttgcatca cagcttttct ttgctccatg gctgtctgca
    35161 ttcgtgcctg cgagcgttgt tccgcctgtg atgtacgatt aactgaaggg gaaatgccac
    35221 tggaaaggga agatgacctg gcgtcgtttg tcattaccgg gtcaacaaac agaattgata
    35281 cgacttcatg ctgatacact tctacctgtt tatagggctc actttctgcc tggcgggtta
    35341 atatctgact tcctctttcc gccataccac cggcaataac gcccgcaaca gcttcaccac
    35401 tgggcattcc cacgcgtccg gtaaccgtgc catagttacc ctgaataacc tgcgtgttgg
    35461 catctttata aagtgtgcca atactgccca cacctcctaa cactgacggc agaatgatat
    35521 gtttaaacca gcgtgtattc acattgctgg ccacggatga catcaggttg tcttcccgtt
    35581 gagcatatgc attgacctta aggtccatgc cgttccagct catccggtcg aaatggatct
    35641 ccacgccgtt acccaccagt ttgactcccg gactgaacag cgttgcccct gcccaggggc
    35701 cagcgggaat ggttgcgata accttggatg acgggttatc agaatccact gcagtatcaa
    35761 tatatgccgg tatacgggtc agggcaggta tcagttgtat ccctttttct gctacaggcg
    35821 tggacaggct ggcattctgt aatgcgaatt gctgcgtacc attctgccct gttgtttctg
    35881 ccggagattt attccacata gtcgttgcta tggttggtgt actaccggca ggatgctgat
    35941 cttttattct tacaatcagc ttttgcagcc gttccatacg cttttcactg gctgctcgat
    36001 cctcctgtgg tgtgccggaa gcctggcgat aagcatagtt ttccggtttt gctgcaggct
    36061 gttgttgctt ttccggagtg tccggaatat caagcccctg cggaagactg gcaatgaacg
    36121 tctgattatt gcgggctgcc gccgcagccc ctcgttcatt atccgctctt aacagttcac
    36181 gatagtgagg cgtttcggta acgctgcgcg taccgccact tgcagcactg tttatgttca
    36241 cggctgactg catatcgctg ttactccctg atactttgga aaacgcgata tagctgccgc
    36301 caaataagat gatgctgctc agcccaagca gggcagccag tttctttccg gatttaccgg
    36361 catcctgttc agcactcatg attactccag ttctggtgtc acgtgaacgg actggccatt
    36421 cacagagaaa gtcagtagtg gggtgacggg aagcttccag agatgagttc cgtcggcaga
    36481 ggacaaggtc tgttcaaatt catcgcgcaa tatcgcccgg gaacgaacca gaaggtcatc
    36541 gccgtgttgc cagatcgttg tatccgggac attacctgtg aatttcagcc tcttaactga
    36601 agggtcccgg ggaggaatac catccagaaa tgcctgtaat gttgcatcat gcagggcgat
    36661 tttatccgtc gggatggaga caaccgggct ggttggtccc tgccgtggta ttcgcagatc
    36721 cagacgactg tccatttcct gactggatga cggcgtatct gtttcgccac tggttacgtt
    36781 caggatgacc ggtaccgaca gacctttcag gtagacagaa atgtttccgg atgcccatgg
    36841 ccgcaatggt gtgattgtca ccatgttctc gttgtactgc acatcaaaga gttttggtgc
    36901 cgcgttataa ggaggatcgg attgtggcca tggtgaaccg ttgatatcgg taaatgtcac
    36961 tacgctcagg ttgttcatgg ctgtgcgaac aagaggaagg ctggcaccgg gggacagatt
    37021 aactgtcaga gagctgatac ggggtacaac actggtgatg ggcgcattga tggcccgctc
    37081 gttatccgcc atgagactgc gtagtgagcg aatctcatcg gcggagagct cagaatcaag
    37141 tcttgccgca tcctgtacca gcgggctggc ctgcccgggg gcaggcagct caccttttac
    37201 cagtgttgcc ggaacacctc cggtgttttg ggaggcgttc tgaacgtgat cgctggcaga
    37261 atttgtcgtt tgtggtgtgc gtgcgttctg ccatcctgcg ttatcggcag acgtggcggc
    37321 gctgcacacc aggagcagtg tgctcagtaa ataacgtgat tgcatattac ttatctcagg
    37381 agttcggtcc ggcgttacgg gagatcatct gccggatttc cataccggat ggtttcagtc
    37441 tggggtcaac acgctggatg gtgatttcga aaacaaaaga ctgttctggc ttgctggttg
    37501 tctggccgac cagacgcata cggacaggat actgaaatgt ccagaaccat acgccgtcgg
    37561 acatttgtcc ctgtcgtacc agaacgccag cgcctgtcgt ggcggtcaga ttcatcttct
    37621 ctttcttgat cgtctccaga atgttagatg cctgtaatgc attcacatat ccggcaaaac
    37681 cttcgtcaga aaaacgcgga gacagggagg aaatctgtga acgatagtgc acaaaatcca
    37741 ggttgaatgc ggtcgccagt gcctggctgc caaaggccat tgcgtcattc tgactccatg
    37801 ctggctgatt tgttggtgcc agcctgacca gacgaccatt gtctgtagcg aaatattcgc
    37861 gctccacatt cgttgagtgc cagtactgaa tggcgttgcc agtaatactg ataagaagac
    37921 atgtgcccgt acataaaagc gcttttatca gcgcagggac attatttgcc cgctgttctg
    37981 actgcttcat agccctgatt gtgctcttca gtatttccgg gtcaggtgcc ggatgtgatg
    38041 gcgaggatag ctcggtattt tcggtcatag gttgtagtcc ttccagggat caatattccc
    38101 cggcagcttt tcattcacct tgtccacgac cgcgtcgcag acctggtttt ccagctgcga
    38161 cagaatgtcg tcaaggctgg ggaactgggg gaggtttatg ctgatttttt tgattcgtga
    38221 cagacaggac tgcagggatg aggaaacatc gttttcacgt tgtgaccatg catccgcagc
    38281 tgcacgtgct cgctcatatc ctgtattact accggcctgt gccgcgctac ccgcacgaca
    38341 ggttgcagca cttgccgtgg atgaaatcag gataaggccg agcacggcaa ccagagactt
    38401 atgcatgttc atatggcttc cttaccggct catcggccgg ggctgtggga gagatcctga
    38461 tgcagagttt tttcctgcct gtcctccggc ttttggctgt tggaccactg ctgccatgac
    38521 tgctcgaagc ccaggttccg gtacaacgca tcaggttgtt tttccggcgt gtagacagca
    38581 gcaaaaagcg gttcttcccg ggttttcaga tgaaactgca taagctccgg tgcggggcga
    38641 ttcgtgctgt cagcatcgtg gtttaccgct tcaccataac ctgcaaagcg taaacctttg
    38701 tcggtcagcg cattgataac caactgactg ttgtcggccg atggggtggc cagtgccgga
    38761 tacaatgtgc cgttcagccg gaccacaccc tgcagatgtt catcggcact ctggacaagt
    38821 agcaggttga gttcacgcgt atccttatcc actgatccca gaacaggtgt cagcttgtca
    38881 ggggctgcgc tggttatttc tgttacctga tttgcgttct gtggcgcgga gaccggatgt
    38941 cggtcttcac cgataaacag atgtgagccg acgttttctg gcatacgtaa cgctttcatc
    39001 tcttcgggcc acccctggct gatttgcgca gccactttca taaaggtttc gaccggaatc
    39061 gcctgaatac gctgaccttt atccggatta tcatgtgata ccgagatccc gttggtaact
    39121 gactccatgg accacttatt ccggaaacgt gctttttgct caccagggag aatgaattgt
    39181 tcgcggttct gaagtgtcag tgtcaccacg tcacctttct gatggttttt gaccagactt
    39241 tccagttgtt ccccccagta gagtttgctg ccactgcggt ttttaagctc aacaaagaaa
    39301 ctctctcctt tgccgggtgc atattcagca cgaccaaaat tgtgcagtac ggcagtcacg
    39361 ctctctcccg ggcgaagttt tcctggtggt tcttcacctt gtgccgcatc tgccgttttg
    39421 ccaggttcag ccggtgatga tgcagggacg gtgctgacgg ttgatgcttc aggggcaact
    39481 ggggacgccg gggattgtgt ggcttctttt tcctgaacgg ggtcagggac aaccggttgt
    39541 tcaggggtat tccggttaag ttccggcgtt ggctgatggg tgacaacagc atccttaccg
    39601 acagccattt ccttacgtaa tgtatcgagc tccagacgct gatcagcgtg tttcatgcgc
    39661 acattgatgt tgtgctcgat aatcaaccgc atcgcttttt gcttaaaggc atcactgcct
    39721 gtcagctcaa tgacgccacc atagaaatta gtggccacag caagtgcggc cagaacactg
    39781 cgatcgtcat tgctggcacc gtcgaccatt tccagccggt ttacccggtc aatgaaggct
    39841 ggtttgccat ccagcttgta caggacggtt ctgtctgctc gtttttcacc cttcagtgat
    39901 tttatgattt cgtccagatt aagcgatatc ggtgcatcag gacgttccgg ggcgtagaca
    39961 atactgtcag gtgtcggtgt ggtcgtcgtg gtttccggtg agggcgtggg atcactgttc
    40021 tcatgtgcct gtgaatccgt ttttttacgc gtaaaaaagc cacgcgcttt gtcgagaaac
    40081 gatgaagtat tatcagaagg tgatgtgctc tgaacaggtt gctgtggatt gggttcatcc
    40141 cggacagccg gttctgcagt atctgttgtg gcctgaagtt ctgactcatc tgcctgaacg
    40201 tggtctgtgt ccggttgcga cggcatatca ctgttttcct gcagatgctc aggattttca
    40261 ttgttcatca gctcctgata tgcgctgtag ttttcgtatt cttcggggcc atctgactca
    40321 ggaaggaacg gctctggctg aaggggaatg tcttcagtta catggctgac cgcctcttcc
    40381 cggggagtat ccggggatat tccggtgctt tgtttgtcat caggctccgt catcacggtc
    40441 atttcctctt tgactgcatc aggggaaggt gatggctcgg ctgattcatt catggtttca
    40501 gatgagggac tgtcttcagg ggatgcgggg gcggcagcag gggccggtat ttcaggtgaa
    40561 gtgccggatt cctgttgtga ctcgtttttt tccggttgtg acagttcggc ttcaaattca
    40621 cgccagtctt tatctgtggc caccgagaca actttttccg gaggttccgc cggatgagcg
    40681 ggttcatcag gggcagatgc cgttgtactt tccggggacg ccggtgattc tgcaggggag
    40741 tcctgaacta cagagctttc cgtttgcgcc tgaacgttat cagcgtgccg ctcatctttg
    40801 ccgttatttt cagtggcgtg actggtaaat gactgtgttt cgtcagcaat gttactgctg
    40861 gaagcagctg gttgttcctg ctgcgtggcg gacggcagag aaggtccctg gtcatggctg
    40921 tgccctgtat cgggtgatgt ttcaggatat tctgtcggag tcatggtttt ttcctcagtg
    40981 tggaccggtg taaaaacgag gttgccgatc accagtgcct cgcggatttc cggggtgtta
    41041 ctgttggggg tggaaacccc gatgtgttct aactgggcgt tgatgacatg ctgaaaagcg
    41101 gcccgtcccc ggcttgcgtc caggtcattg aaatcggtca gcccctgcgc tttttcggca
    41161 tcggtgaacg caggaaagat gaccgttcct cctgtcagtt ctgcggcttt tgttgcactg
    41221 acgatacctt tgttaacccg gattgcatga tcgttgtcgg cacagaagat aaaggggctt
    41281 tgcgtccact tttgccgggc attttcggca acggcaatca tgttgcctcc atccaccgtc
    41341 atcagtaccg gaaggcccgt ggcttcgtgc agggaggccg cagtggcata gccctcggca
    41401 aacagtaccg gctgaccgtt gcgcggtgtg cccagcgcaa accagttacc ggttttctct
    41461 gaatctttca ggatgcgggc atccttcccc ccggtgaccg gaatacgctg gtaagagcgt
    41521 atcgcgccgt tacggttact gaagggaata accagttcat ttttgttgtt tacccgcacc
    41581 cccggtgcag cctgaatgcc tttccgggtc aggtattcat gtgctgtggc ctgcggccat
    41641 ttgttgacat agcggcgggc atacgcggcc tgtcggttgt actgtgcttt cagttcccgc
    41701 tccgcgtcct ccctgcgttg cattgaatgc gctttcaggt ggagtcttgc gcggggatct
    41761 gtctgttctc cgccggagaa ggtccaggtg atcggcgaat catccgcgct gcgataatcc
    41821 ctgtaccagc cggcaggtct gccgtccagg aatccgcgat atgcgccact tttttgtcct
    41881 tttttgtcat cggcagtcgg gacacggtgg attttcccgt ccatgaccgg cagttctttc
    41941 agaaccagtc cggcattctc cagaacctgg gcaaactcgg tgaccggatc actgccgttc
    42001 atggagacgt cctgtggccg gggcagccag cggtccagcc tggacaggtc cgcccccggg
    42061 cgggcgaacc agagtttgtg ctccttgtcc cagatgacgg gcgtgtgccc gttcatcggg
    42121 ggatgtgctt ttttgagatc ttctatttca tctgcaggca cggcaagcca ggtgcggtag
    42181 cgggatacag acggcatgag tgcttctcct gaaaaaagac ggaaaaaaac caccgccgga
    42241 agccgggggt ggctgggggg atagaaaatc gggggagaaa tgtttgtcag ggtttccagt
    42301 ctgtaccctt attccagcga tcctgccagt gttcaagata ttgtgcggct acatccggca
    42361 tattcttgag cacaacgaca ttctctgaat tcttgcgtgc agcagcacga gagaagttat
    42421 aggagccagt ttcaaccgtg tttccgtcaa caatgatgac tttgtcgtga tggatgggga
    42481 aggcatctac agtccggaga ggaatgtcca gcaggttgat atatttcatc gcttcctgac
    42541 ttgcccggtt cgtattcccc ttgtcatcaa caacaatacg gacgtccact ccgcgttttt
    42601 tcgcttttgc cagcgcatgc attacatccg ggtcggtgaa ggaatatgcc atcatcctga
    42661 tggagctttc cgcactgttg attgtgtcaa gcaccagagc ggatgcgctt ccttcaggag
    42721 aaaagccgac cttcacggat ggcgtgtcga tcattgcaaa cgatggtgtc gtgaacagta
    42781 acgggcatgc cagtagcgat acccggagca gagtaacgat agttttttta cctgacataa
    42841 agactccaga atttaatcga ggccagtcca gtctcgtggt gattccgtat ggtggcgata
    42901 ccaccatgga cgaccggagg cgatggcccc tctcagaatg ctgaaaatgc gtgtacagat
    42961 agtcctgaca gtccagcctg aacggtcgag aataacgaaa aataaaatga tgccggtgca
    43021 aatccagaag gttgttttgg agggaaaggg gcatataaac agataaatca tggtgaacgg
    43081 cagaggtacg ccctggatgt tgactggcgg tgttgcatat cgccagaaac cttctctttt
    43141 catgaccgta actcccctgg cgggataaaa agaatcgctt ctcttcggtc aacttcattc
    43201 cggatataca tttccagaat ctggtcccgg atacggcgct tctcctgccg gattatggca
    43261 tcaatgtgat gcccccactc tgcatgcggc attccggaaa gattgtcgcg aagctcatcg
    43321 ttaatgacga tatattcccg gacggcttta cgaccaccgt catttgttct gaccagaacc
    43381 tgaaccagaa tgaactgcaa cagtgacagg acagcccagg ccatcgaatc cctgatgaca
    43441 gggggaaaca gcccaagaag acgtgccagc gtttcaccag gagatttcgt atgcatggtg
    43501 ctcaggcagt aatgcccggt gttgcctgcc tgtaccgctg cgtcggcggt ttcgttatcc
    43561 ctgatctcac caacaccgat aatttcggga ttacgtcgta cagcagaacg cagtccggca
    43621 gcaaaactga ccacatcacg cccgatttct gcctgatgcg gtgggagcag atcgttttca
    43681 tttcccagga tatattccac cgggtcttca tatgtcacga ttttggcgtc ggggaaattg
    43741 tccataatgt aacggtacag tgctgaacac agcgttgatt ttcctgaccc ggtttcacca
    43801 catacaaatc ccagaccgct tttgcagacc attgcttcaa gcaggtctgg ttcaatgttc
    43861 atggagagaa tgtccggtat ttctgtcggg atgacacgca tggttattga gattgctttc
    43921 tcctccgcac cagatgtgcc ctgaatgagg tgagagcgta accggatgcg ttctccgcgt
    43981 ttaagaccat agcgcccgga tgcatccccg ttaatctgta tcgtcctgtc aaccggattg
    44041 cctgccagaa cgcggggaat gacttcccga ccgaacagtt catcaatcag ggaggacatg
    44101 agtgtggttg gtaacgttgc actggaacaa cgcacccgtc ggccaaagcg actgacagat
    44161 acaggtgacc cgccggtcag atccacatca ctgactttat gtgcggcaca ccagacaaag
    44221 aaatgccgta gttcatctgc agtgaaacgc tgaaacggaa aaagaccaaa ctcatcagga
    44281 atattcatcg ggagcctcct tcagtgccta taagcggacg ccagtgagat ttctgaagat
    44341 tgaattccgc atgattttta aggcgacgaa cacgatctcc agtgaccagt tttttcccgt
    44401 cacctgtaac ggtttgcatt tgatcagaaa catccggacg ggtgatcatg ccctgacgcc
    44461 agagaagtga gtacagcatc atgccccggt aatcacgggt gagggttttc tggttggctt
    44521 caagcgtcag gtcggcgttt tgtcttcctt cctgccatcc ctgccgtaat gcggtttccc
    44581 acacttttcg ttgcacgctg tcttctggaa cgacgctgcc ttctgtaccc ggagtggctg
    44641 ttgttgaaag cccccggaga agccagtcac gccagcccgg tggattgctg acaaagcgtt
    44701 cagggcgaat gatggtccag acccgggaag aggtccggat ttgatctggc gtgatatgag
    44761 ctacgtcctg tgcttcatca ataaccggtg gtagccagcc ttccgggcta atcagtggcc
    44821 ggaaatcgta taaggcatta agtgtgcttt cccgggcgtt gagcgcctga atcagctccc
    44881 atgaacgctg agccttgcca ccacgaaatc caagcgtttt acctgcgtct gtcagcattt
    44941 tccagcgtgt ttctgacaga ctatccggct tttcacttcg tggtttaagc catgcattaa
    45001 tgtcaggtgg tggcgtattg acaccacctg gcgagatggt aggggaagaa gaacaaccag
    45061 taagcagtat tactggcaaa aggagagagg tgagtgcttt cattttttcc ggttctccgg
    45121 ctgcatgaag ctgacctgta acaagcccgg atattgatgg attgttgctc tccaggctgt
    45181 ctgggcttca ataagtctca acgtattgga aaacgtcata tcccggacgt gaaggttgac
    45241 tggtaacggc aggcggacac cattgtagtt aaactgcatc cccctgactc tggccagttc
    45301 gttcagtaat tcaattgcat caccttccca gtcaaaggaa aggcgatcgc tgttagctgt
    45361 cagaagatga gaccccgttg aaaccggaag ttggccggaa aatacacctt tgtcccataa
    45421 tgtgtactga gtgtgccggg taaccgccac atcagaggca gcaccgtagt tacgcggaac
    45481 aggagtatgc tgagaagggg agttgcagcc agccagaaaa agggcggcaa gacaccccgc
    45541 aggtataata cggttcataa taattcctga gcagttaaat cagaatggga tataccggtg
    45601 ctccggtaca gacaggatga tgtttgtttc cggctatact tgcgttaccg gaaataaatg
    45661 ccagtgtggc caggtaaata gcctcatagt ttactgaagg tgattattcc tgatactggc
    45721 tggttgatgt gtcgtcagac ataaaacgaa tatgagcgta atctttaacg ctccgcgccg
    45781 aatcgatcca tgatggatga ctcgacgatt tatgaatcga acgtaccggc gcttgtccct
    45841 gaaacagttc ccggccctcg tccggataac ctgcaattca gacaggcaca tgtgcccgta
    45901 cactcttgta tcttactgag tatatcagaa tttatgtaaa ttcaaatatc ctctatgcct
    45961 ctttgttgta ttttccgtca agtatgagcc gggtaccatc gtaatgcatg atcagggcat
    46021 caaggtacgc cggtttgaat ttcttgccga gcctatctct ggccagaaa
//
LOCUS       DAPGOF010000014        45977 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.014, whole genome shotgun sequence.
ACCESSION   DAPGOF010000014 DAPGOF010000000
VERSION     DAPGOF010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45977
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.014"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            195..1766
                     /gene="garD"
                     /locus_tag="RHV13_000687"
     CDS             195..1766
                     /gene="garD"
                     /locus_tag="RHV13_000687"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HDV1240247.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAANEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            1914..2249
                     /gene="prlF"
                     /locus_tag="RHV13_000688"
     CDS             1914..2249
                     /gene="prlF"
                     /locus_tag="RHV13_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HDV1240248.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGLDNIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFDIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            2249..2713
                     /gene="yhaV"
                     /locus_tag="RHV13_000689"
                     /pseudo
     CDS             2249..2713
                     /gene="yhaV"
                     /locus_tag="RHV13_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
     gene            complement(2768..3577)
                     /gene="agaR"
                     /locus_tag="RHV13_000690"
     CDS             complement(2768..3577)
                     /gene="agaR"
                     /locus_tag="RHV13_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HDV1240249.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            3826..5106
                     /gene="kbaZ"
                     /locus_tag="RHV13_000691"
     CDS             3826..5106
                     /gene="kbaZ"
                     /locus_tag="RHV13_000691"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HDV1240250.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKATALSQMVESYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YNGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            5129..5602
                     /gene="agaV"
                     /locus_tag="RHV13_000692"
     CDS             5129..5602
                     /gene="agaV"
                     /locus_tag="RHV13_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HDV1240251.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            5613..6392
                     /locus_tag="RHV13_000693"
     CDS             5613..6392
                     /locus_tag="RHV13_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HDV1240252.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            6382..7260
                     /locus_tag="RHV13_000694"
     CDS             6382..7260
                     /locus_tag="RHV13_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HDV1240253.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            7278..7712
                     /locus_tag="RHV13_000695"
     CDS             7278..7712
                     /locus_tag="RHV13_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDV1240254.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKQGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            7709..8842
                     /gene="nagA"
                     /locus_tag="RHV13_000696"
     CDS             7709..8842
                     /gene="nagA"
                     /locus_tag="RHV13_000696"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDV1240255.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCFNGMT
                     GLHHREPGMVGAGLTDKRAWLELIVDGHHVHPAAMSLCCCCAEERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            9193..10347
                     /locus_tag="RHV13_000697"
     CDS             9193..10347
                     /locus_tag="RHV13_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HDV1240256.1"
                     /translation="MPENCTPAAAATGTWTEEEIRQQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFAVL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESSDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            10360..11220
                     /gene="kbaY"
                     /locus_tag="RHV13_000698"
     CDS             10360..11220
                     /gene="kbaY"
                     /locus_tag="RHV13_000698"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HDV1240257.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            11387..11863
                     /gene="agaB"
                     /locus_tag="RHV13_000699"
     CDS             11387..11863
                     /gene="agaB"
                     /locus_tag="RHV13_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HDV1240258.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            11902..12705
                     /gene="agaC"
                     /locus_tag="RHV13_000700"
     CDS             11902..12705
                     /gene="agaC"
                     /locus_tag="RHV13_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HDV1240259.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVANKNEEEDYSNGI"
     gene            12695..13486
                     /gene="agaD"
                     /locus_tag="RHV13_000701"
     CDS             12695..13486
                     /gene="agaD"
                     /locus_tag="RHV13_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HDV1240260.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            13487..14254
                     /locus_tag="RHV13_000702"
     CDS             13487..14254
                     /locus_tag="RHV13_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HDV1240261.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDVSQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGENLQPACHISQLDARTQQHEMLKTAGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTSIPASFLWLHNHFTCMIDEICRH"
     gene            complement(14584..15528)
                     /locus_tag="RHV13_000703"
     CDS             complement(14584..15528)
                     /locus_tag="RHV13_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV1240262.1"
                     /translation="MNSIRENDLNRVDLNLLIVLLVLHREGSVSRAASKLHLGQPAVS
                     GALARLREMFNDPLFVRTAKGMAPTPRAQDLVTALLPVMDQMQQVIFQRPVFTPEQDQ
                     HTFRLGMSDWVERWLMPGMMADIAQMAPGIRLQVTVSDPFQDGEKVQQDKVDVAISVV
                     EQVPAGVEREVITQMGFCTMWHPLQLSLTSPLSLDVYTEIDHLLVSYRGASSSSIDLI
                     LADCGRKREVRYVTPNFSSLPLMLRQIPALATVPQGLAEGWVADYGLQFADVPLEVPA
                     FTLSLLWNKQRNNDEALRWLLEKLRARMQGWEMFSECV"
     gene            15610..16566
                     /locus_tag="RHV13_000704"
     CDS             15610..16566
                     /locus_tag="RHV13_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001718913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDV1240263.1"
                     /translation="MKLLLTLVTSLVLSVNALATPIPVPSPDTQAPGYYRMILGKLRI
                     TAVSDGTVTIPLDKLLTHISADKLRQQLAKDSLTPHTETSINAFVIDNGQQRVLIDAG
                     AGDLFGNNGGQLLENLAAAGYPAETIDAVLLTHIHADHSGGISRQGKPAFPNAQVFVE
                     QKDADFWLNPANASQVEDSQKHTFVESEQTIRPVINAGQLKTFIAPATVIAGIAATST
                     AGHTPGSVMYTVESEGQTLVLWGDIIHAKAVQMPDPHVAIHFDVDQQQAVKTRERVLK
                     HAAQGGYWVAAAHISFPGLGHVKSDSKGYRWVAANYTTQLAP"
     gene            complement(16676..17539)
                     /gene="rsmI"
                     /locus_tag="RHV13_000705"
     CDS             complement(16676..17539)
                     /gene="rsmI"
                     /locus_tag="RHV13_000705"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDV1240264.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGECRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEDDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQGE"
     gene            17604..19640
                     /gene="lpoA"
                     /locus_tag="RHV13_000706"
     CDS             17604..19640
                     /gene="lpoA"
                     /locus_tag="RHV13_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HDV1240265.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGAML
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            19598..19993
                     /locus_tag="RHV13_000707"
     CDS             19598..19993
                     /locus_tag="RHV13_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HDV1240266.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            20013..20603
                     /gene="diaA"
                     /locus_tag="RHV13_000708"
     CDS             20013..20603
                     /gene="diaA"
                     /locus_tag="RHV13_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HDV1240267.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSTRIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            20613..21188
                     /gene="yraP"
                     /locus_tag="RHV13_000709"
     CDS             20613..21188
                     /gene="yraP"
                     /locus_tag="RHV13_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HDV1240268.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(21302..22342)
                     /locus_tag="RHV13_000710"
     CDS             complement(21302..22342)
                     /locus_tag="RHV13_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDV1240269.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPLLNPAT
                     LVFMGFVLGWGFVAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(22415..23050)
                     /locus_tag="RHV13_000711"
     CDS             complement(22415..23050)
                     /locus_tag="RHV13_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HDV1240270.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            23178..23696
                     /gene="yhbO"
                     /locus_tag="RHV13_000712"
     CDS             23178..23696
                     /gene="yhbO"
                     /locus_tag="RHV13_000712"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HDV1240271.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(23676..24119)
                     /locus_tag="RHV13_000713"
     CDS             complement(23676..24119)
                     /locus_tag="RHV13_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HDV1240272.1"
                     /translation="METLTAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARQAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRGSGTEQA"
     gene            24170..24472
                     /gene="yhbQ"
                     /locus_tag="RHV13_000714"
     CDS             24170..24472
                     /gene="yhbQ"
                     /locus_tag="RHV13_000714"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HDV1240273.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPKIKSD"
     gene            complement(24459..24962)
                     /locus_tag="RHV13_000715"
     CDS             complement(24459..24962)
                     /locus_tag="RHV13_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDV1240274.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(24956..25480)
                     /locus_tag="RHV13_000716"
     CDS             complement(24956..25480)
                     /locus_tag="RHV13_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HDV1240275.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            25689..26684
                     /locus_tag="RHV13_000717"
     CDS             25689..26684
                     /locus_tag="RHV13_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDV1240276.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMASNIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            26693..27571
                     /locus_tag="RHV13_000718"
     CDS             26693..27571
                     /locus_tag="RHV13_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDV1240277.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELTSMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLLLTANS
                     DCNGYWRRLAGLELQA"
     gene            27777..28784
                     /locus_tag="RHV13_000719"
     CDS             27777..28784
                     /locus_tag="RHV13_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HDV1240278.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(28901..30145)
                     /gene="mtr"
                     /locus_tag="RHV13_000720"
     CDS             complement(28901..30145)
                     /gene="mtr"
                     /locus_tag="RHV13_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HDV1240279.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPAWAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(30299..32188)
                     /gene="deaD"
                     /locus_tag="RHV13_000721"
     CDS             complement(30299..32188)
                     /gene="deaD"
                     /locus_tag="RHV13_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HDV1240280.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFSGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(32181..32261)
                     /gene="yrbN"
                     /locus_tag="RHV13_000722"
     CDS             complement(32181..32261)
                     /gene="yrbN"
                     /locus_tag="RHV13_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HDV1240281.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(32368..33252)
                     /gene="nlpI"
                     /locus_tag="RHV13_000723"
     CDS             complement(32368..33252)
                     /gene="nlpI"
                     /locus_tag="RHV13_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HDV1240282.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(33361..35496)
                     /gene="pnp"
                     /locus_tag="RHV13_000724"
     CDS             complement(33361..35496)
                     /gene="pnp"
                     /locus_tag="RHV13_000724"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDV1240283.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(35743..36012)
                     /gene="rpsO"
                     /locus_tag="RHV13_000725"
     CDS             complement(35743..36012)
                     /gene="rpsO"
                     /locus_tag="RHV13_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDV1240284.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(36161..37105)
                     /gene="truB"
                     /locus_tag="RHV13_000726"
     CDS             complement(36161..37105)
                     /gene="truB"
                     /locus_tag="RHV13_000726"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDV1240285.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(37105..37506)
                     /gene="rbfA"
                     /locus_tag="RHV13_000727"
     CDS             complement(37105..37506)
                     /gene="rbfA"
                     /locus_tag="RHV13_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDV1240286.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(37670..40342)
                     /gene="infB"
                     /locus_tag="RHV13_000728"
     CDS             complement(37670..40342)
                     /gene="infB"
                     /locus_tag="RHV13_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDV1240287.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(40367..41854)
                     /gene="nusA"
                     /locus_tag="RHV13_000729"
     CDS             complement(40367..41854)
                     /gene="nusA"
                     /locus_tag="RHV13_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDV1240288.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(41882..42334)
                     /gene="rimP"
                     /locus_tag="RHV13_000730"
     CDS             complement(41882..42334)
                     /gene="rimP"
                     /locus_tag="RHV13_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDV1240289.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(42541..42617)
                     /locus_tag="RHV13_000731"
     tRNA            complement(42541..42617)
                     /locus_tag="RHV13_000731"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42581..42583),aa:Met,seq:cat)
     gene            42965..44308
                     /gene="argG"
                     /locus_tag="RHV13_000732"
     CDS             42965..44308
                     /gene="argG"
                     /locus_tag="RHV13_000732"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HDV1240290.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENLENKGQ"
     gene            complement(44316..45941)
                     /locus_tag="RHV13_000733"
     CDS             complement(44316..45941)
                     /locus_tag="RHV13_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HDV1240291.1"
                     /translation="MTVFNKFARSFKSHWLLYLSVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYSPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKNAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQCNPTPEGQG"
BASE COUNT        11123 a        12246 c        11630 g        10978 t
ORIGIN      
        1 acataaggtt gcgcgctaaa gcacagattt gcgctttacc ttgccgggcg gcactgcaat
       61 ccctgaaatg attgacattg atcacatttc tgcgtttaaa ctcctgacat tcttatttca
      121 cccaatgaag tcatttattt ttaaatgaga ccaggtcctc attttaataa cccctggctg
      181 gagaatattg cacaatggcc aacatcgaaa tcagacaaga aacgccaact gcgttttata
      241 taaaagttca cgacacagat aatgtggcaa ttattgttaa tgataatggc ctgaaagcag
      301 gaacgcgttt tccggatggg ctggaattaa ttgaacatat tccccagggg cataaagtcg
      361 cattgctgga cattccggct aatggtgaaa ttattcgtta tggcgaagtg attggttacg
      421 ccgtgcgtgc aatcccacgc ggaagctgga tcgacgaatc aatggttgta ctgccggaag
      481 cgccgccgtt acacacgctg ccactggcaa ccaaagtccc ggaaccctta ccgccgctgg
      541 aaggatacac ctttgagggc tatcgcaatg ccgatggcag cgtgggcacc aaaaacctgc
      601 tcggcatcac caccagcgtc cactgtgtgg caggcgtggt ggactacgta gtaaaaatca
      661 ttgaacgcga tctgctaccg aaatacccga acgtcgatgg cgtggtgggg ctgaatcatt
      721 tgtacggttg tggcgtggcg attaacgcac cggcggcagt tgtgcctatt cgtaccattc
      781 acaatatttc gctgaaccct aactttggcg gcgaagtaat ggtgattggc ctgggttgtg
      841 aaaagttgca gcctgagcgc ctgctgactg gaacggatga tgtgcaagct attccagtag
      901 aaagcgccag cattgtcagt ttgcaggatg aaaagcatgt cggttttcag tccatggtcg
      961 aggatatttt gcaggtcgcc gaacgccatc tacaaaaact gaatcaacgt cagcgagaaa
     1021 cctgtccggc ttcagaactg gttgtcggca tgcagtgcgg tggcagcgat gccttttctg
     1081 gcgtaacggc aaacccggcg gttgggtatg cgtctgatct actggtgcgc tgcggcgcaa
     1141 cggtgatgtt ctcagaagtc acggaagtgc gtgacgcgat ccatctgctg acaccacgcg
     1201 cagcgaacga agaggtcggc aaacggctgc tggaggagat ggagtggtac gataactatc
     1261 tcaatatggg aaaaaccgac cgcagcgcca acccttcgcc gggcaacaag aaaggcggtc
     1321 tggcaaatgt agtggagaaa gcgctcggct ccattgctaa atcgggtaaa agtgcaattg
     1381 ttgaagtgct gtcgcccggt caacgcccga ctaaacgcgg attaatttac gccgcgacgc
     1441 cagccagcga ttttgtctgt ggcacgcaac aggtggcttc gggtatcacc gtgcaagtgt
     1501 ttacgaccgg tcgtggtacg ccgtacggcc tgatggcggt acccgtcatt aaaatggcga
     1561 cccgcaccga gctggcgaac cgctggtttg atttaatgga tattaacgcg ggcactatcg
     1621 ccaccggcga agaaaccatt gaagaggtgg gctggaagtt gttccacttt attctcgacg
     1681 tcgccagcgg gaagaagaaa accttctcgg atcaatgggg actgcataac cagctggcgg
     1741 tgtttaaccc ggcaccggtg acctgatttc ttttggaacg tccctcgcaa aacatggcct
     1801 tagtgccatg tttttattgt ttaaagcccc cacgtccatt aataatgcat ttgcattact
     1861 ttgctgaagc ttataatttg agcagaaaac aggctgtaaa agtacagtgc ctaatgcccg
     1921 ctaatgctcg ctctcacgct gtactgacca ctgaatcaaa ggtcacgata cgcggacaaa
     1981 caactatccc cgcgccagtg cgtgaggcct taaaactgaa gccaggcctg gacaacattc
     2041 attacgaaat tctgcctggt gggcaagtat ttatgtgccg actgggagat gaacaggagg
     2101 atcatactat gaatgcattt ttgcgttttc tggatgcaga tatccagaac aacccgcaaa
     2161 aaactcgtcc attcgacatt caacaaggaa agaaacttgt cgctggcatg gacgtcaaca
     2221 ttgatgatga gattggcgac gacgaataat ggattttcca caaagggtta atggttgggc
     2281 gctatatgct catccctgtt ttcaggaaac ctacgacgct ttagttgccg aagtcgagac
     2341 attaaaggga aaagatcctg aaaattatta gagaaatgcc gccacaaagt tattggcagc
     2401 agttcacaaa gtgattgagg agcatatcac ggtcaatcca tcatcaccgg cattccgtca
     2461 tggcaagtcg ttaggctctg ggaaaaataa agactggtca cgggtaaaat ttggtgctgg
     2521 tcactatcgt ctcttctttc gttacagtga aaaagagaaa gtcatcattc tgggatggat
     2581 gaacgatgaa aacacgctgc gcacctacgg taaaaaaaca gatgcctata ccgtattcag
     2641 taaaatgtta aaaagaggac atcctcctgc cgactgggaa agcctcaccc aagagacaga
     2701 agaaaaccat tgatggtgtt cacaatgccc gccgaacgca ttcgtgttga ccagcgggca
     2761 ccctttctca ctccccgacc agaatcactt caaccccagc ctttcgcagt ccttccagac
     2821 tatccgcagg aatgccttca tcaacaatga tcatgtcgat acgttgagta tcaatgatct
     2881 tatgtaaact ggagcggttg aacttactgg aatcggtgac tacgatgatc cgttccgcaa
     2941 cttcgcacat ccggcggttt aaacgggctt catcttcatt atgcgtgctg acgccgcgct
     3001 ccagatcaat cgcatctaca ccaagaaaca gcatatcgaa gtggtaattt tgcagcgatt
     3061 gttcagcctg atcgccgtaa aaagattgcg actgacggcg caaatgcccg ccggtcatca
     3121 gcagctcaac gccttccgct tccagcaaag cattagccac gttcataccg ttggtcatcg
     3181 caattacgtc agtgtgcttg cgcatcagac gagcaatctc aaaagtggtg gtcccggaat
     3241 cgaggatcac ccgatgacct ggctgaatca actcaacggc agctttcgca acgctgcgtt
     3301 tcatcgcggt gttcagtgcg cttttatctt ccactgatgg ctcgactgac ggcgtcgtgc
     3361 tatcgcagat caacgcgcca ccataggcac gcacagcgat cccctgcttt tccagaaacg
     3421 ccagatcgtt gcggatcgtc acggtagata cgccatacaa tgccgacaga tcgttaacct
     3481 gcacactccc ttgctgtcgc agacgctgaa tgatctgttc tcgtcgctcg ctggtgcctg
     3541 tcactcgctt ctcacctgaa gcgtcggtat tactcatagt aagtcctttc gtaaaacttt
     3601 cgtttcattt cgttttgcct attaacgcct ttctattaag caaatgcaag cccaccttgc
     3661 ccattgacgc aagctactct cgtttcagtg actttcatta tgtttctttt gtgaatcaga
     3721 tcagaaaact attatctttc gttttatttt tatctcacca tgacgcagta tcaattgaaa
     3781 caaaacgaaa gattaatatc gcagtaatct gaactggaga ggaaagtgaa acatctgaca
     3841 gaaatggtga gacagcacaa agcgggcaaa acaaatggaa tttatgccgt ttgttccgca
     3901 catccgctgg tgctggaggc tgcaatccgc tacgccagtg caaaccaaac gccgttactg
     3961 attgaagcaa cctccaatca ggtagaccag ttcggcggtt ataccggaat gacgcccgcc
     4021 gattttcgcg gctttgtttg tcagctcgcc gactcgttga atttcccgca ggatgcgttg
     4081 attctgggtg gtgaccatct ggggccaaac cgctggcaaa acctgccggc cgctcaggca
     4141 atggccaatg ccgatgattt gattaaaagc tacgttgcgg caggattcaa aaaaatccac
     4201 cttgattgca gcatgtcctg tcaggacgat ccgattccct taactgatga cattgtggct
     4261 gaacgcgccg cccgtctggc gaaagtggcg gaagaaacct gtcttgaaca ctttggcgaa
     4321 gccgatctgg agtatgtcat tggtaccgaa gtgccggtac ctggcggcgc gcatgaaacc
     4381 ttaagcgagc tggcggtcac cacgccggat gccgcccgcg ccacgctgga agcccatcgt
     4441 cacgcctttg aaaagcaagg tttgaatgcc atctggccac gcatcattgc cctggtggtt
     4501 caacccggcg tcgaattcga tcacaccaac gttattgatt atcagcccgc caaagcgacc
     4561 gccttaagcc agatggtcga aagctacgaa acgctgattt tcgaagcgca ctctaccgat
     4621 tatcaaacgc cgcaatcgct gcgccagctg gtgattgacc actttgccat tctgaaagtt
     4681 ggcccggcgc tgaccttcgc cctgcgtgaa gctctgttct ctctggcagc gattgaagaa
     4741 gaactggtgc cagcaaaagc ctgttctggt ctgcgtcagg tgctggaaaa cgtgatgctc
     4801 gaccgcccgg aatactggca aagccactac aacggtgacg gcaacgcgcg tcgtctggcg
     4861 cgtggttata gctactcgga tcgcgtgcgc tattactggc cggacagcca gattgatgac
     4921 gctttcgctc atctggtacg taatctggcg gattcaccaa ttccgctgcc gctgatcagc
     4981 cagtatctgc cgctgcagta cgtgaaagtt cgctccggcg agctgcagcc aacgccacgg
     5041 gaactcatta tcaaccatat tcaggacatc ctggcgcagt accacacagc ctgtgaaggc
     5101 caataagcaa aacaaagagg aacacgctat gccaaatatt gttttaagcc ggattgatga
     5161 acgcttgatt cacggtcagg tcggcgttca gtgggtcgga tttgcggggg caaatctggt
     5221 gctggtagcc aacgatgagg ttgccgaaga tccggtacaa caaaacctga tggaaatggt
     5281 actggcagaa gggatcgccg tacgtttctg gacgctgcaa aaagttatcg acaacattca
     5341 tcgcgccgcc gatcgacaga aaatcctgct ggtttgtaaa acacccgccg atttcctgac
     5401 gctggtgaaa ggtggcgttc cggtgaatcg cattaacgtt ggcaatatgc actacgccaa
     5461 tggcaagcaa caaatcgcca aaacggtttc tgtggatgcg ggcgatatcg cagcatttaa
     5521 tgacctgaaa gccgctgggg tggaatgctt cgttcagggc gtcccgacag agcctgctgt
     5581 ggacctcttt aaattacttt gagggattca tcatggaaat cagtctgttg caggcattcg
     5641 cgttgggcat tatcgccttt atcgctggcc tggatatgtt taacggctta acccatatgc
     5701 accgcccagt ggtcctcggc ccgttagtcg ggctggtact tggcgatctg cataccggta
     5761 ttttaaccgg cggtacgctg gaactggtgt ggatggggct ggccccgctg gcgggcgcac
     5821 agccgcctaa cgtgattatc ggtactatcg tcggcacggc gtttgccatt actactggcg
     5881 tgaaacccga tgtcgcagtc ggtgtcgccg tacctttcgc tgtcgcagta cagatgggga
     5941 ttaccttcct gttctcggtg atgtctggcg tgatgtctcg ctgcgaccgg atggcggaga
     6001 acgccgatac tcgcggcatt gaacgcgtaa actatctggc actgctggca ctcggcactt
     6061 tctattttct ctgcgctttc ctgcctatct acttcggcgc ggaacatgca aaaaccatca
     6121 ttgatgtcct gccgcaacga ttaatcgacg gcctcggcgt cgcgggcggc atcatgccag
     6181 caatcggttt tgccgtgctg ctgaaaatca tgatgaaaaa cgtctacatc ccctacttca
     6241 tcctgggctt tgttgccgca gcctggctca agttaccggt gctggctatt gctgccgccg
     6301 cgctggcgat ggcgctgatc gatctgctgc gtaaatctcc tgaaccgact caacctgcgg
     6361 cacagaaaga ggaatttgaa gatggcatct aatcaaacca ccctgccgaa cgtcagtgaa
     6421 aacgaagaaa cattgctgac tggcgtcaat gaaaacgtgt atgaagatca gagcattggc
     6481 gcggagctga cgaaaaaaga tatcaaccgc gtcgcctggc gttccatgct gttacaagcg
     6541 tcttttaact acgaacgtat gcaggcttct ggctggttgt acggtctgct gcctgctctg
     6601 aaaaagatcc acactaataa acgcgacctg gcgcgcgcca tgaaggggca tatgggtttc
     6661 ttcaataccc atccgtttct ggtgacattt gttatcggca ttatccttgc gatggagcgt
     6721 tctaagcagg acgttaacag tattcagagc accaaaattg ccgtcggtgc gccgctcggc
     6781 gggattggcg atgcaatgtt ctggctaacg ctactgccga tttgtggcgg gattggagcc
     6841 agtctggcat tgcaaggctc cattcttggc gcagtcgtct ttattgtgct gttcaacgtg
     6901 gtacacctgg ggctgcgttt tggtctggcg cattatgctt accgcatggg cgtggcggcg
     6961 attccactca ttaaagcgaa taccaaaaaa gtcggccatg ctgcgtctat cgttgggatg
     7021 acggtaatcg gtgcgctggt ggcaacctat gttcgtttaa gcaccacgct ggaaatcaca
     7081 gcgggcgacg cagtggttaa gttacaggct gatgttatcg acaaactgat gcctgccttc
     7141 ttaccgttgg tctacaccct gaccatgttt tggttggtac gccgcggctg gagtccgctg
     7201 cgtctgattg cagttaccgt ggttctcggc atcgtcggta aattctgcca tttcctttaa
     7261 atacaatgag gtattagatg ttaagtatta ttttgacagg gcatggcggg tttgccagcg
     7321 gcatggaaaa agcaatgaag caaattctcg gtgagcaatc gcagtttatc gccatcgatt
     7381 ttccggaaac ctcaagcacc gcgctgctta catcgcagct tgaagaggcc atcgcccaac
     7441 tggattgtga agacggcatt gtttttctga cggatttgct gggcggcacg ccgtttcgcg
     7501 tagcttcaac gctggcgatg caaaaacagg gctgtgaagt gatcaccggc accaatttgc
     7561 aactgctact ggagatggtg ctggagcgcg aagggttaag cggtgaagaa ttccgtgtac
     7621 aggcgctgga gtgtggacat cgtgggctga ccagtctggt ggacgagtta ggccgctgcc
     7681 atgaagaatg tccggtcgag gaagggatat gacacacgtt ctgcgcgcca gaaggctgct
     7741 gactgaagag ggatggctcg atgaccatca gttgcgtatt gctgacggtg tcatcgcagc
     7801 aatcgaaccg attccagtgg gcgtgactga acgcgatgcg gaactgctct gccccgctta
     7861 catcgacacc catgtacacg gtggtgcggg cgttgatgta atggatgacg cgccggatgt
     7921 gctcgacaag ctggcaatgc acaaggcacg cgaaggtgtc ggcagttggt taccgactac
     7981 cgtaaccgcg ccgcttaata ccattcatgc ggcgctgaaa cgtattgcgc aacgttgcca
     8041 acgcggcgga cctggtgcgc aagtgctggg gagttatctc gaaggaccgt acttcacgcc
     8101 gcagaataaa ggcgcgcatc cgccggagtt gtttcgcgag cttgaaattg ccgagctgga
     8161 tcaattgatt gccgtttctc agcacacctt acgcgtggta gcgctggcac cggaaaaaga
     8221 gggggcattg caggccatcc gccatcttaa acagcaaaac gtacgagtga tgctggggca
     8281 tagcgcggcg acctggcaac aaacccgcgc cgcgtttgat gctggtgccg acggcctggt
     8341 gcattgcttt aacgggatga caggtttaca tcaccgcgaa ccgggaatgg ttggcgcggg
     8401 attaacggac aagcgcgcct ggctggaact gatagtcgat ggtcatcatg tacatccggc
     8461 ggcgatgtcg ctgtgttgtt gctgtgcgga agagagaatc gtgctgatca ccgacgcgat
     8521 gcaggcagcc gggatgccgg atggtcgcta tacgttatgt ggtgaagaag tgcagatgca
     8581 cggtggcgtt gtccgtaccg cgtccggtgg gctggcgggc agtacgctgt ctgttgatgc
     8641 ggcagtgcgc aacatggtcg agttgacggg cgtaacgcct gcggaagcca ttcatatggc
     8701 gtcgctgcat ccggcgcgaa tgctgggtgt tgatggtgtt ctgggatcgc ttaaaccggg
     8761 caaacgcgcc agcgtcgttg cgctggatag cgggctacat gtgcaacaaa tctggattca
     8821 gggtcaatta gcttcgtttt gatggtttgc tccattattg ggccttcact tcccccgtaa
     8881 ggcctttctt tttctttctt tttgatctgt gcagcggtgt cggatgcgac gctaacgcgt
     8941 cttatccgac ctacgatacg ccgcatccgg caatcggtga acatagcgac gtcattacgc
     9001 aattctttaa tcttcccttg tcttttcttt caatgatcac agatttcgtt ttatttcctt
     9061 tttctccatt gaactttcga tttcttttct atagatttta atcaacgaaa gacatcacca
     9121 agtgaaatga aacgaaaggt aagcgaaagc gacaacgccc gacgtcaagt tcatcagact
     9181 aaggattgag ttatgccaga aaattgcacc cctgctgccg ccgcaaccgg tacatggact
     9241 gaagaagaga tccgccaaca gcctcgcgca tggatccgtt cactcaccaa catcgacgcg
     9301 ctacgttccg cgctcaataa cttccttgaa ccgttactgc gcaaagagaa tctgcggatc
     9361 atcctgaccg gagccggaac ctcagcattt atcggtgaca tcatcgcgcc gtggctcgcc
     9421 agccataccg gtaaaaactt cagcgccgta ccgaccaccg atctggtcac taatccgatg
     9481 gactacctga acccagccca tccgctgctg ttgatctcct tcggtcgatc cggcaacagc
     9541 ccggaaagcg tcgcagccgt ggaactggca aatcaatttg taccggaatg ctatcacctg
     9601 ccgatcacct gcaacgaagc gggcgctctt taccaaaacg cgatcaacag cgataacgcg
     9661 tttgccgtgc tgatgcccgc agaaacgcac gatcgcggct ttgcgatgac cagcagcatt
     9721 accaccatga tggccagctg cctcgcggtt ttcgcacctg agacgatcaa cagccaaacc
     9781 ttccgcgacg tggcggatcg ttgccaggcg atcctgacct cactgggcga tttcagcgaa
     9841 ggtgtgtttg gttacgcacc gtggaaacgg atcgtttatc tcggcagcgg tggcttacag
     9901 ggcgcagcac gcgagtcggc gctgaaagtg ctggaactga cggcgggtaa actggcggcc
     9961 ttttatgatt ctccaaccgg attccgtcat ggaccaaaat cgctggtcga taacgaaaca
    10021 ctggtggtgg tatttgtctc cagccaccct tacacccgtc agtatgatct tgatctgctg
    10081 gctgaacttc gccgtgacaa ccaggcaatg cgtgtaatcg ccatcgccgc ggaaagcagc
    10141 gacatcgtcg ctgccggtcc acatatcatc ctgccaccgt cacgtcactt tatcgacgtt
    10201 gagcaggcat tttgcttcct gatgtacgcc cagacgtttg cactgatgca gtcgctgcac
    10261 atgggcaata cgccggatac cccatcagcc agtggcaccg ttaaccgcgt ggtgcaaggc
    10321 gtaatcattc atccgtggca ggcataagag gatcgcatta tgagcattat ctccaccaaa
    10381 tatctgttac aggacgccca ggccaatggc tacgcggtgc ctgcttttaa cattcataac
    10441 gccgagacga tccaggcgat cctcgaagtg tgcagtgaaa tgcgatcgcc ggtgatcctc
    10501 gccggaacgc cggggacctt taaacatatc gcgctggaag agatctacgc cctgtgcagc
    10561 gcctattcca caacctacaa catgccactg gcgctgcatc tcgaccacca cgaatcgctg
    10621 gatgatattc gccgtaaagt ccacgcaggt gtgcgcagtg caatgatcga cggcagccac
    10681 ttcccgtttg ccgagaacgt gaagctggtg aaatcggttg ttgacttctg ccactcacag
    10741 gattgcagcg tggaagcaga actgggccgc ctgggcggtg ttgaagatga catgagcgtt
    10801 gacgccgaaa gtgcattcct aaccgatcca caagaagcta aacgctttgt cgaactgact
    10861 ggcgtcgaca gcctggcggt agcgattggt acggcgcacg gcttatacag caaaacgccg
    10921 aagattgatt tccagcgact ggcggaaatt cgtgaagtgg tggatgttcc tctggtgcta
    10981 catggtgcca gcgatgttcc ggatgaattt gtccgtcgca ctattgaact tggcgtcaca
    11041 aaagtgaacg ttgccacaga attaaaaata gccttcgccg gcgcggttaa agcctggttt
    11101 gcggaaaatc cgcagggtaa tgatcctcgt tattatatgc gcgtcggaat ggatgcgatg
    11161 aaagaagttg tcagaaataa aattaatgtc tgtggttcag cgaatcgaat ttcagcataa
    11221 tcattagttt tttatgattt atcccaatgt acttcccgat taatatcggg gagtgcctta
    11281 atggaaaagg agatgactaa accttaataa atacatcagt acaatatcgc aacaataata
    11341 tatttaaaaa aattatatta ttcaacttta tggtgaggat tacacaatga gcagtccaaa
    11401 tattctctta acccgtattg ataaccgtct ggttcacggt caggttggcg tgacctggac
    11461 atccaccatc ggtgcaaatc tgctggtagt cgtggatgat gttgtcgcta acgatgatat
    11521 tcaacagaaa ttaatgggta ttaccgcgga aacctacggc tttggcattc gtttctttac
    11581 tatcgaaaaa accattaacg tcatcggcaa agctgcacca catcagaaga tcttcctgat
    11641 ttgccgtacg ccacaaacgg tacgtaaatt ggtagaaggt ggcattgacc tgaaagatgt
    11701 caacgtcggc aatatgcatt tctcggaagg gaaaaagcaa atcagcagta aagtttatgt
    11761 cgatgaccag gatctcgcgg acttacgttt tattaaacaa cgtggcgtga atgttttcat
    11821 tcaggacgtc cctggcgatc aaaaagaaca aatccctgac taaatctaaa atcgccttaa
    11881 tattggtttg aggtaataaa aatgcatgaa ataaccctac ttcagggatt atccctggcg
    11941 gcgttagttt tcgttctggg gattgatttt tggctggaag ccttattttt attccgcccg
    12001 ataatcgttt gtaccctaac tggcgctatt ctcggtgata ttcagactgg cttaattacc
    12061 ggtggtctga cagagttggc tttcgccgga ttaacccctg caggtggtgt tcagccgccc
    12121 aacccgatta tggcgggtct gatgaccacc gtcattgcat ggtctacggg cgttgatgcc
    12181 aaaacagcaa ttggtcttgg cctgccgttt agtttgttaa tgcagtacgt cattctgttc
    12241 ttctattccg ctttctcatt atttatgacc aaagccgata aatgcgcgaa agaggcggac
    12301 acggcagcgt tttcccggct taactggacg acgatgctca tcgtcgcttc agcgtatgcg
    12361 gtgattgctt tcctctgtac ttacctggca caaggggcga tgcaggcgct ggtgaaagcg
    12421 atgcccgcct ggttgaccca cggctttgaa gtggctggcg gtattctgcc tgccgttggt
    12481 tttggcttgc tgctgcgcgt aatgttcaaa gcgcaatata tcccttacct gatcgccggt
    12541 ttcctgtttg tttgctacat ccaggtcagc aacctgttgc cggttgccgt actgggcgca
    12601 ggctttgcgg tgtatgagtt tttcaatgcg aaatcccggc agcaagcgca accgcagccc
    12661 gttgccaata aaaatgaaga agaggactac agcaatggga tctgaaatca gtaaaaaaga
    12721 tatcacccgt ctgggctttc gttcgtcgct gctgcaagcg agctttaact acgaaaggat
    12781 gcaggcgggc ggttttacct gggcgatgtt gccgatcctg aaaaagattt ataaggacga
    12841 caaaccgggc ttaagcgcgg cgatgaaaga taacctcgaa tttattaata ctcacccgaa
    12901 tctggtcgga ttcctgatgg ggttattaat ttcgatggaa gaaaaaggag aaaaccgcga
    12961 caccattaaa ggcctcaaag tggcactgtt tggcccaatc gccgggattg gcgatgcgat
    13021 tttctggttt actttgttgc cgattatggc gggaatttgc tcatcatttg ccagccaggg
    13081 aaacctgctg gggccgattc tatttttcgc cgtttacctg cttatctttt tcctgcgcgt
    13141 cggctggacc cacgtcggtt attcagtcgg cgtgaaggcg atcgataaag tgcgagagaa
    13201 ctcgcagatg attgctcgct cggcaaccat cctcgggatc acggtaatcg gcgggctgat
    13261 cgcttcgtat gtgcatatta acgtggtgac atcgtttgcc atcgacagta cccacagcgt
    13321 cgcgctgcaa caggatttct tcgataaagt cttcccgaac attttaccga tggcctacac
    13381 cctgctgatg tattacttcc tgcgggtgaa aaaagcgcat ccggtgctgt taatcggcgt
    13441 gacttttgtg ctctctattg tttgttccgc attcggcatt ttgtaaatgg aacgaggcac
    13501 tgcgtctggc ggtgcctctt tacttaagga atttcatcct gtgcaaaccc ttcagcaagt
    13561 tgaaaactat acggcgttaa gtgaacgtgc cagcgaatat ttattggccg tgatccgtag
    13621 caaaccggat gccgtgattt gcctggcgac cggagccacg ccattactga cgtatcatta
    13681 tctggtagaa aaaatccacc agcagcaggt tgatgtcagc cagctcacct tcgtgaagct
    13741 cgacgaatgg gtggatctgc cattaacgat gcctggtacc tgcgaaactt tcctgcaaca
    13801 gcatatcgtg cagccgctgg ggctacgtga agaccagctc atcagctttc gctctgaaga
    13861 gataaatgag acagagtgcg aacgggtaac gaacctgatt gcgcgcaaag gcggtctgga
    13921 tttatgcgtt ctcggattgg ggaaaaacgg tcatcttggg ctgaacgaac cgggagaaaa
    13981 cctgcaaccg gcctgccata tcagtcaact tgatgccaga acacagcaac atgagatgtt
    14041 aaaaaccgcg ggtcgccccg tgactcgtgg gatcacctta ggcctgaagg atattctcaa
    14101 tgcccgcgag gttttgttac tggtgactgg cgaaggaaag caggatgcga cagaacgttt
    14161 tctcacggct aaagtctcta cctctatccc ggcttcgttt ttgtggctgc ataaccattt
    14221 cacatgcatg attgatgaga tttgtcgtca ttaacatccc tcttagcccg gaaaaataag
    14281 aaaagtgctc atgtcttttg agcacttcat tacggttcag tttgatcgtc ccccgctatt
    14341 attccatcac agtgtaaacc taaaggttga caaaaaatac ataatatcag gtacatggtg
    14401 acagagcaca gctccctcat acgactcact aatcctgaaa agcgattcaa atacttatta
    14461 acccaattag ctgcaagaca tgtttaacgg ccattagggt acgattgcac ctatacgcta
    14521 aattatctga caggagttat ttaatttagc aagcccttat ttcctgttca catttttacg
    14581 ttttcaaacg cattctgaaa acatctccca gccctgcatt cgtgcgcgta atttctccag
    14641 taaccagcgc agcgcctcat cattattacg ctgcttattc cagagtagag agagcgtaaa
    14701 agcaggaacc tcgagtggga catcggcaaa ttgcaaccca taatcggcta cccagccttc
    14761 tgccagcccc tgcggtacgg tcgctaaagc cggtatctgt ctcaacatca acggtaacga
    14821 tgagaaattt ggcgtgacgt aacgcacttc ccttttacgc ccgcagtcag ccaaaatcag
    14881 atcgatagag ctactgctgg caccgcgata agagaccaaa agatgatcga tttcagtata
    14941 tacatcaagg cttaacggtg aagtgaggga taactggagc ggatgccaca tcgtgcaaaa
    15001 ccccatttga gtgatcactt ctcgttccac tccagcgggc acctgctcca cgacggagat
    15061 tgctacgtcc accttatcct gttgcacttt ttcgccatcc tggaaaggat cggataccgt
    15121 gacttgtaag cgaatcccgg gtgccatttg ggctatatct gccatcattc caggcatcag
    15181 ccagcgttcg acccagtcac tcatccccag gcgaaacgta tgctgatcct gttctggggt
    15241 aaaaactggc ctttgaaaaa tcacctgctg catttgatcc atcacaggca gtagtgccgt
    15301 aaccagatct tgtgcacgtg gtgtcggtgc catccccttt gccgtgcgca caaataacgg
    15361 gtcgttaaac atttctctca gccgtgccag cgcgccactt actgctggct gccccaagtg
    15421 taacttgctg gctgcacgtg aaacgctccc ctctcgatgt agcaccagga gcacgattag
    15481 caggttgagg tcgacccggt ttaaatcatt ttctctgata gagttcatta tattaatcaa
    15541 tttgaatgat gtaattcgca aaaggatact ggtgcccgta ctgtaataac agaacttatc
    15601 gaggatagaa tgaaactttt gctgactctg gtgacctcac tggtactctc cgtaaatgcg
    15661 ttagcaacgc ctatacctgt gccttctcct gatacacaag caccgggcta ttaccgcatg
    15721 atattgggca aactacgtat taccgctgtc tccgacggca cggtgacaat tccgctagat
    15781 aaattactca cccacatttc tgccgataaa ctgcgccagc aactggcaaa ggattcactg
    15841 acaccccata cggagacttc aatcaatgca tttgttattg ataatggcca gcagcgcgtg
    15901 ttgattgatg ccggtgcggg cgatctgttt ggcaacaatg gaggccaact gcttgaaaac
    15961 ctggcagctg ctggctatcc ggctgaaacc attgatgccg tgctgctgac gcatattcat
    16021 gccgaccact ctggtggcat ctcccgtcag ggcaaaccag ctttccctaa cgcccaggta
    16081 ttcgttgagc agaaagatgc ggatttctgg cttaatccgg cgaatgcctc gcaagtagaa
    16141 gatagtcaga agcacacctt tgtcgaatcg gagcagacca tccgcccagt gatcaacgca
    16201 ggtcagctga agacctttat cgcaccggca acggtaattg caggcattgc agccacctca
    16261 acggccggcc atactcctgg tagcgtgatg tacacagtgg agagtgaagg ccagacgcta
    16321 gtgttgtggg gcgatattat tcatgccaaa gcagtgcaga tgccggatcc tcacgtggcg
    16381 atacatttcg atgtggatca acagcaagcg gtaaaaaccc gtgagcgagt attaaaacat
    16441 gcggctcagg gtggttactg ggtggcagct gcccatatct cattccctgg cctggggcat
    16501 gttaaaagcg acagcaaggg ttatcgttgg gtcgctgcca actataccac gcaactagcc
    16561 ccctgattta agaggtcagt ccgcacggag cgaggattta cggcggtatt cactaaaaag
    16621 actaattgtt tagcaggcgg cccctgggtt cttcaaacgg ggccacggag agatatcact
    16681 ctccctgctg ctccagcgca tacttataca gcgcattttt cttcacgccg tgaatttctg
    16741 ctgccagcgc cgccgctttt ttcagcggca gttctgcctg caacagagcc agcgtgcgca
    16801 gggcatcagc aggcaggtca tcttcctgcg ctttatggcc ttcgacaatc agcaccattt
    16861 cgcctttgcg acggttttca tcttctttga cccacgccag cagctcgcca acgggcgcgc
    16921 cgtgaatggt ttcccaggtt ttggtgagct cacgcgccag aaccacgtag cggcattcgc
    16981 ctaataccgc aacgatatct tccaggctat ctaacagacg gtgggtagat tcataaaaaa
    17041 tcagcgtgcg cggctccgct tcaatggctt ttagcgcatc acggcggcct tttgatttgg
    17101 caggtaaaaa gccttcataa cagaaacggt cagagggtaa acccgcggcg cttaacgcag
    17161 tgatagcagc acacggtccg ggtagcggca ccacacgaat ccccgcttca cggcaggtac
    17221 gcaccagatg gtagccagga tcgttaatta gcggcgttcc ggcatcggaa accagcgcaa
    17281 tgttttgccc ctcttgcagc ttcgccagca gcgtttcggc tttttgttgt tcgttatggt
    17341 cgtgcagcgc aaacaaccgg gcattaatcc caaaatgttg cagcaataaa ccggtgtgac
    17401 gagtatcctc ggcggcaatc agatcaacgg cctgtaatac ctctaacgca cgctgggtga
    17461 tatccgccag attgccgatt ggcgtcggta caatgtaaag ctggccctga gaattatccg
    17521 ccgattggtg ttgtttcatt gtgtcgtccg tattgccgat ttaatattga gcattgcgta
    17581 aaaaaaatat cactggatac attatggtac cctcaacatt ttctcgtttg aaagccgcgc
    17641 gttgtctgcc tgttgttctg gcagccctga ttttcgccgg ttgtggcacc catactcccg
    17701 atcagtccac tgcttatatg cagggcacgg cgcaggctga ttctgccttt tatcttcagc
    17761 agatgcagca aagctctgat gataccagga tcaactggca attactcgcc attcgtgcac
    17821 tggtgaaaga aggtaaaacc gggcaggcgg ttgagttgtt taaccaacta ccgcaagaac
    17881 tgaacgattc tcagcgtcgc gagaaaacac tgctggcggt agagattaaa ctggcgcaga
    17941 aagattttgc tggcgcgcaa aacttgctgg cgaaaatcac acctgccgat ttagaacaaa
    18001 accagcaagc gcgttactgg caggcaaaaa tcgatgccag ccaggggcgt ccttccattg
    18061 atttactgcg cgcgttaatt gctcaggaac cgctgctcgg cgcgaaagaa aaacagcaga
    18121 atattgatgc cacctggcag gcgctctcct ccatgactca ggaacaggcg aatacgctgg
    18181 tgatcaacgc cgacgaaaat attctgcaag gctggctgga tctgcagcgc gtctggtttg
    18241 ataaccgtaa cgatcccgac atgatgaaag ccgggatcgc cgactggcaa aaacgttatc
    18301 cgaacaatcc gggcgcgaaa atgctgccaa cgcagttggt taacgtaaaa gcgtttaaac
    18361 cagcctcgac caacaaaatc gccctgctgt tgccgctgaa tggccaggca gcggtatttg
    18421 gtcgcactat tcagcaaggc tttgaagcgg cgaaaaatat cggcactcag ccagtggcgg
    18481 ctcaggtagc tgccgcacct gccgcagacg tagctgaaca acctcagccg caaaccgtgg
    18541 atggcgttgc cagcccggca caagcctcgg ttagcgatct gaccggtgaa cagcctgcgg
    18601 cccagccggt gcctgtaagc gccccggcca caagcaccgc agcggtaagc gcacccgcaa
    18661 atccatccgc agagctgaaa atctacgata cctcatcaca accacttagc cagatcttaa
    18721 gccaggttca gcaggatggc gcgagtattg tggttggtcc gttgctgaaa aataacgttg
    18781 aagagttgct gaagagcaac accccgctga acgtactggc actgaaccag ccggagaata
    18841 tcgaaaaccg cgtcaatatt tgttacttcg cgctttcacc ggaagacgaa gcgcgcgatg
    18901 cagcgcgtca tattcgtgac cagggtaaac aagcgccgct ggtgctgatc ccacgcagtt
    18961 cattgggcga tcgcgtagcc aatgcgtttg cgcaagagtg gcagaaactg ggcggcggca
    19021 ccgttctgca acaaaaattt ggttccacca gcgaattacg cgcgggtgtt aacggcggtt
    19081 ctggtatcgc tttaacgggt acaccgatta ctcccagagc gacaaccgac tccggcatga
    19141 cgaccaacaa tccaacgctg caaaccacgc caaccgatga ccagttcacc aataatggcg
    19201 gtcgtgtcga tgcggtgtac attgtggcaa cgccgggtga aatcgctttt atcaaaccga
    19261 tgatcgccat gcgtaacggt agccagagcg gtgcaatgct gtacgccagc tcccgcagcg
    19321 cacaaggcac cgcaggcccg gatttccgtc tggagatgga aggtttgcag tacagcgaaa
    19381 tcccgatgct ggcgggcggt aatctgccgt taatgcagca ggcactcagc gcggtgaata
    19441 acgattattc actggctcgc atgtatgcga tgggcgtcga tgcctggtcg ctggcaaatc
    19501 atttctcaca aatgcgccag gttcagggtt ttgaaatcaa cggtaatacc ggaagcctga
    19561 cggcaaaccc ggattgcgtg attaacagga agttatcatg gctacagtac caacaaggtc
    19621 aggtagtccc cgccagttaa ccaccaaaca gaccggcgat gcgtgggaag tacaagcgcg
    19681 tcgctggctg gaaggcaaag gactgcgatt tgtcgccgct aacgtgaacg agcgtggcgg
    19741 cgagatcgat ctgataatgc gtgaaggccg gaccaccgtt tttgtcgagg tgcgttaccg
    19801 ccgttctgcg ctttatggcg gcgcggcagc cagtgtgacc cgcagcaaac aacacaaatt
    19861 attacagact gcccgcttgt ggctcgcgcg tcataatggg agttttgata ctgtggattg
    19921 ccggttcgat gtggtagcct tcaccgggaa tgaggttgag tggattaagg atgcctttaa
    19981 tgaccactca taattaaggt ttaaggatta gcgtgcaaga aagaattaaa gcttgcttca
    20041 ctgaaagcat tcaaactcaa attgcggcgg cagaggcgct tccggatgcc atctcccgtg
    20101 cggccatgac gctggttcag tctctgctca atggcaacaa aatcctctgt tgtggtaatg
    20161 gaacttccgc tgccaatgca cagcattttg ctgccagcat gatcaaccgt ttcgaaacgg
    20221 agcggcccag cttacctgcc attgcactaa atactgataa tgttgtctta acggcgattg
    20281 ccaacgatcg cttacatgat gaagtgtatg caaaacaggt gcgggcgctg ggtcatgcgg
    20341 gagatgtatt gttagccatt tccacccgtg gcaacagccg cgatattgta aaagcggtag
    20401 aagctgccgt tacgcgtgat atgaccattg tggcattgac cggctatgac ggcggtgaac
    20461 ttgcaggttt gttagggcca caggatgtgg agatccgcat tccttcgcat cgtagtactc
    20521 gcattcagga aatgcatatg ctgacggtaa attgcctgtg cgatctgatc gataacacgc
    20581 ttttccctca ccaggatgat taaggagaat acatgaaggc attatcgcca atcgcagtcc
    20641 ttatttccgc gctgctgttg caaggttgtg ttgccgctgc cgtagtgggt accgctgctg
    20701 tgggtaccaa agccgcaact gacccacgca gtgtcggcac ccaggtggac gatggtaccc
    20761 tggaagtgcg cgtgaacagc gcattgtcga aagacgaaca gattaagaaa gaagcgcgca
    20821 ttaatgtaac ggcctatcag ggcaaagtgc tgctggttgg gcagtcacca aacgctgaac
    20881 tttcggctcg cgccaaacag attgctatgg gcgtagacgg tgccaacgaa gtgtataacg
    20941 agattcgtca gggccagccg attggtctgg gcgaagcgtc taatgatacg tggatcacca
    21001 ccaaagtgcg ttcgcagctc ttaaccagcg acctggtgaa atcgtccaac gtgaaagtga
    21061 ccaccgaaaa cggtgaagtg ttcctgatgg ggctggtgac tgaacgtgaa gcgaaagcgg
    21121 cggcagatat tgccagccgg gtgagcggcg tgaagcgggt aactacggcg tttacgttta
    21181 ttaaatagca gccccttgta atgcctgatg cgacgcttgc cgcgtcttat caggcctaca
    21241 acgatgcaaa ccgtaggtcg gataaggcgt ttacgccgca tccgacatgt tttccctcaa
    21301 atcagaacaa cagcgccagc ccgccgacaa tcacaccaga cactgccacc atcgcccctg
    21361 tcagccataa ggctttcgcc gggaacgctt tgcgcagcat aatcagtgac ggcaaactca
    21421 ctgccgggag cgtcatcaac aatgccagcg ccggagcggt tcccatacct gccagcatca
    21481 tcgtttgtac aatcggaatt tctgctgccg tgggaatgac aaacaagcat cctgctaccg
    21541 ccatcgccac cacccacatc aggctgttat cgacagcacc atcggcatgg gggaataacc
    21601 agacgcgagc ggcacccaac accagtactg caaggatgta aaccgggatc gtactccaga
    21661 aaagcgtcca tagcgccctg ccccagcggc taaaaaatcc gccctgtgct tccggtatgt
    21721 caatttcgac cggtgcttgc gtttgcggtg tttcacgcac ccatttttgc accagcgtcg
    21781 caatcagcaa caccatcacc agccccgcca ccagacgaat cgccacaaac ccccagccga
    21841 ggacgaaccc cataaacacc agcgtcgccg ggtttaacaa cggattgccc atccagaatg
    21901 ccagcgcacc gcccatcgac acctgttggc gacgcattcc cgccgcgacc ggagccgcac
    21961 agcaggtaca catcatgccc ggcagcgaaa acagcgttcc cagcagcgtg ccgcgaaagc
    22021 gcgattgccc aagcgtacgc aacaaccagt cacgcgggat cagcacctga atcaacgaac
    22081 cgaggatcac ccccagcacc gccgctttcc ataccgcgag gaaatagatc atcgcgtaat
    22141 ccaacgccgc ctgccatgga ttagcatccg cctgcgcaag gatagattta ccgatactgt
    22201 gggtttcggc agcagtaaac gctttgccgt agtaaggttg ccatttcaca taccagagac
    22261 cggcaatgac aacgagaaag aaaagcgcgg gtttccacca ctgaatgggc gttgccgcct
    22321 gagatgaaga ctgaccagtc atagcattcc ccaggaataa gttatgataa ttgagcgcgt
    22381 gaatattacg ctcactatca attcttggga ataattattc agctcttttg cgtaattctg
    22441 aagagcttaa aatcgtcaca ccttcatgct cgggccgcat cgcctctgcc agcatgaccc
    22501 gcgccacgtc tctcgcatca atggatttcc agttaccggg taacaaacgg aaaagcggcg
    22561 caaaaagcgt ttcgttcatc cgctgtttgc tacgatcgcc cagtaacatc gacgggcgag
    22621 caatggtcaa tttcggccag ttctgggcga ttaatgcttc ttccatctcc cctttgacgc
    22681 ggttatagaa aaacggcgag tgggcattgg cacccatagc actgactacc agcatatgct
    22741 gcgcgcccag tcgccgcccg gttaatgcgg tatccactac cagcgtgtaa tcggcatgaa
    22801 taaacgcctc tttgctcccc gcttctcgcc gcgtggtgcc gagacaacaa aacacaatgt
    22861 cgatgggatc ggtgacctgc gccagcgcgt cagtcagttg cggatcatgg ggattaaaca
    22921 ccccgggcat atcgcccaac ggacgtcgcg tcggcgcggc tatggcgtta actttcggtt
    22981 cgttaatcaa catccgcagc aggtgaccgc ccaccagccc cgttgcgcct gtaatcagta
    23041 cctgactcat cttcgctcct ttacagaatt gtctgccttg cgctccacgg ctcatgcacc
    23101 aggcttaata gaccatgagg taattatccc cgattgtggg aaattcgcct catccaatgc
    23161 aacaacgcgg aggaagcatg agtaagaaaa ttgccgtttt aatcactgat gaatttgaag
    23221 attcagaatt tacttcaccc gcagacgagt tccgtaaagc cggacacgaa gtgattacca
    23281 ttgaaaaaca agcgggtaaa acggtgaaag gcaaaaaagg agaagccagc gtgaccatcg
    23341 ataaatccat cgatgaagtg acgccagcgg agtttgatgc cctgttgcta ccgggtggtc
    23401 attcaccgga ttatctgcgt ggcgacaacc gttttgtcac ctttacccgt gattttgtga
    23461 atagcggcaa acctgttttc gccatctgtc acggtccgca gctgctgatc agcgccgatg
    23521 ttattcgggg acgcaaactg accgcggtta aaccgatcat tattgatgtt aaaaacgcgg
    23581 gcgcggaatt ttacgatcag gaagtcgtgg tcgataaaga tcagctggtt accagccgga
    23641 cgccggacga tctgccagcg tttaaccgcg aggcgttacg cctgctcggt gcctgagccg
    23701 cgtagccaaa tcattttttt accaaagccc agcgtgttgt cggtgaattt gatttcatcg
    23761 agccggattt cccacaccgg ggccgacagc attctggcaa ccggaaagcg acgattgtac
    23821 gcctggcgcg caaggtcgct ttcctcacct tcaagcctgc ggatctcgcc tttaaattgc
    23881 acgccgcgaa ttaacgctac cgttttcggc tgaccgttta ccgttccggc aactgccgcc
    23941 tgcggcccac tcatctgcgc gtggcgcgtt ttttcttccg tcaaaatgta gaaggcgact
    24001 ttctgcgcat caaaaagata aaaggcatta gcgcaccaga gttccccttc ctgctgcaca
    24061 caccaggtga caacatgttg ttttgccagc caacggctga tggcggtgag tgtttccatt
    24121 gctgttctct tttatactgt gggcctgacc ttaacatgcg acgcagacga tgacaccctg
    24181 gtttctttac ttgatccgta ccgccgacaa taagctttat accgggatca ccacagatgt
    24241 cgaacgccgc tatcagcagc accaaagcgg caaaggggcg aaagcactgc gcgggaaagg
    24301 agaactaacg ctggcgtttt ccgcgccagt gggcgatcgt tcgctggcac tacgggcgga
    24361 atatcgcgtt aagcaactga ccaaacggca aaaagagcgt ctggtagcgg aaggcgcagg
    24421 gtttgcagaa ctgttaagca gcctgcaaac cccaaagatt aaaagcgatt gaagtgctcg
    24481 tgatactcaa ccagaccggt tacgccgttc agcgcgtcat ccgctaaacg atgtacctgg
    24541 aaggcgcttt cagtgcccgg ccagcggcaa cgcagatcgt gatgcgccgc cagctcaaaa
    24601 ccaaaacgac tgtacagcgc cggatcgccc agcgtcacca ctgcggcata gccgaactca
    24661 ttaagcgaat cgagtccttc atagaccagt tggcgtgcca gcccctgtcc acggtatttt
    24721 tcatcaaccg ccaacggtgc catgccgacc cattgcaggt cttcgccctg cacatcaacc
    24781 ggactaaatg ccacatagcc aatgacctga ccttcgtcat ctgtcgccac cagccccagc
    24841 gtcagaaagc catcttcacg cagatcgtga accagcttcg cttccgcatc actttcgaat
    24901 gaacgacgca gcagggcatc aatacccggc gcatcaatgg gaatttctac tcgaattagc
    24961 atggttcacc taccgatgtc tgtttggttt caggcgcggt tttcattccc gcctcaacaa
    25021 aatccgccag ttgcagcagc atcatgcgta aggctttcgg catttgctcc agttcaatgg
    25081 cgtccatcag gtttttcaca tacagcccca gttccgtatc gccttcaatc accagccgac
    25141 gctggaagaa gagcgtatcc ggatcttgtt tacgcgccgc aatcatcagc agatcgctgg
    25201 cgtcggcact aaaactcaca tcagcttgcg cgttctggct gaccaccagt ttgccattca
    25261 ccaccgaggt aaaccattgc aggtcaatat cacgcacatg aatacttaac cagcggcctt
    25321 caaggaactc cagctcacca tcatccagcg cctggcggaa ttgccagctt aagacctgct
    25381 caagaacctg gcgttttagc gcaaatggcg tcagttttac cggtacactc aacagagatg
    25441 gtcccaaatg cacaatgcgg gaacgcagtt tatccaacac gagttttact ccctgtttca
    25501 acaatcatcc tattttgcca tatcagaaaa ataacatagc ggtataaatc aacaattcca
    25561 tatgaaattg ctgctaccac caatacaact ttaactgcct taaatcaaaa attgtcgcag
    25621 caaggttaac taaaatccca gttcgttaac atttttgcgt tttgatagcg caaccttcag
    25681 gaaaaattat ggagctgctc tgccctgccg gaaatctccc ggcgcttaag gcggccatcg
    25741 aaaacggcgc agatgctgtt tatatcgggc taaaagatga taccaatgcc cgtcacttcg
    25801 ccggccttaa ctttaccgag aaaaaattgc aggaagcggt gagttttgtc catcaacatc
    25861 gccgcaaact tcacatcgcg attaacactt ttgcgcatcc ggacggttac gcgcgttggc
    25921 agcgcgccgt ggatatggcg gcgcagctgg gtgccgacgc gctgatcctc gccgacctcg
    25981 ccatgctgga gtacgccgcc gagcgttacc cgcatattga gcgtcatgtg tcagtgcagg
    26041 cttcggcgac caatgaagag gcgattaact tttatcatcg ccattttgac gttgcccgcg
    26101 tggtgctgcc gcgcgtgttg tcgattcatc aggtgaaaca gctggcacgg gtcacacctg
    26161 tcccgctgga agtctttgct tttggcagcc tgtgcattat gtcggaaggc cgttgctatc
    26221 tgtcgtcgta tctgacgggt gagtcgccca acactgtagg tgcgtgttct ccggcccgtt
    26281 tcgtgcgctg gcagcaaacg ccgcaggggc tggaatcccg cctgaacgaa gtgctgatcg
    26341 accgttatca ggacggcgaa aacgcaggtt atccgacgtt gtgtaaaggg cgttatctgg
    26401 tggacggcga gcgctatcac gcgctggaag aaccaaccag tctcaatacc ctggaactgc
    26461 tgccggagtt aatggcgtcg aatatcgctt cggtgaaaat tgaaggccgc cagcgtagcc
    26521 cggcgtatgt cagccaagtg gcgaaagtct ggcgtcaggc tatcgaccgt tgtaaggccg
    26581 atccgcaaaa cttcgtaccg caaagcgcgt ggatggagac gctcgggtcg atgtccgaag
    26641 gcacgcagac cacccttggc gcgtatcacc gtaaatggca gtgagaagag caatgaaata
    26701 ttccttaggg ccagtgctgt ggtactggcc aaaagagacg ctggaagatt tttatcagca
    26761 ggccgccacc agcagcgccg acgtgattta tcttggcgaa gcggtttgca gcaagcgccg
    26821 ggcaaccaaa gttggcgact ggctggagat ggcaaaatcg ctcgccggaa gtggtaagca
    26881 gatagtgctc tccacgctgg cgctggtgca ggcatcctct gaactgggcg aactgaaacg
    26941 ctatgttgag aacggtgagt ttctgattga agccagcgat ctcggcgtgg tgaatatgtg
    27001 cgctgaacgc aaactgccgt tcgtcgccgg gcacgcgctg aactgctaca acgcggtgac
    27061 gctgaaaata ttgctcaaac agggcatgat gcgctggtgt atgccggtgg agctttcccg
    27121 cgactggctg gtgaatctgc ttaatcagtg cgatgagctg ggcattcgca accagtttga
    27181 agtggaagtc ctgagctacg gtcatctacc gctggcctac tccgctcgct gctttaccgc
    27241 gcgttcggaa gaccgcccga aagatgagtg tgaaacctgc tgcattaagt atccgaacgg
    27301 gcgcaacgtg ctgtcgcagg aaaaccaaca agtgtttgta ctcaatggca ttcagaccat
    27361 gagcggctac gtttacaacc tcggtaacga gctgacatcc atgcagggat tggttgatgt
    27421 ggtccgcctg tcaccgcagg gtactgacac tttcgcgatg ctcgacgcct tccgcgctaa
    27481 tgaaaatggc gctgcgccac tattgttgac ggcaaacagc gactgtaacg gctactggcg
    27541 gcggctggca ggactggagc tgcaagccta agtaaatatg ctcgtcatac ttcaagttgc
    27601 atgtgctgca tctgcattcg ttcaccccag tcacttactt atgtaagctc ctggggattc
    27661 actctcttgt cgccttcctg caactcgaat tatttagagc acagctcact ttgttaacaa
    27721 ctttaactac tctttaatgc agtattaaag attaatcggt aacaaagtga gctgttatga
    27781 ctgataaaac cattgcgttt tcgctactcg atctggcccc cattcccgaa ggttcttcag
    27841 cgcgagaagc attctcccac tctctcgatc tcgcccgtct ggctgaaaag cgcggctatc
    27901 atcgctactg gctggcagaa caccacaata tgactggcat tgccagtgct gccacgtcgg
    27961 tattgattgg ctatctggcg gcgaatacca ccacgctgca tctgggttct ggcggcgtga
    28021 tgttaccaaa ccactcaccg ttggttattg ccgaacagtt tggcacgctc aatacgctct
    28081 atccggggcg aatcgatttg gggctgggtc gtgctccggg tagtgaccag cgaaccatga
    28141 tggcgctacg tcgtcatatg agcggggata ttgataattt cccccgcgat gtcgcggagc
    28201 tggtggactg gtttgacgcc cgcgatccca atccgcatgt gcgcccggta ccaggctatg
    28261 gcgagaaaat ccccgtgtgg ttgttaggct ccagccttta cagcgcgcaa ctggcggcgc
    28321 agcttggtct gccgtttgcg tttgcctcac acttcgcgcc ggatatgttg ttccaggcgc
    28381 tgcatcttta tcgcagcaac ttcaaaccgt cggcacgact ggaaaaacca tacgcgatgg
    28441 tgtgcatcaa tattatcgct gccgacagca accgcgacgc tgaatttctg tttacctcaa
    28501 tgcagcaagc ctttgtgaag ctgcgccgtg gcgaaaccgg gcaactgccg ccgccgattc
    28561 aaaatatgga tcagttctgg tcaccgtctg agcagtatgg cgtgcagcag gcgctgagta
    28621 tgtcgctggt gggcgataaa gcgaaagtgc gtcatggctt gcaatcgatc ctgcgcgaaa
    28681 ccgacgccga tgagattatg gttaacgggc agattttcga ccaccaggcg cgcctgcatt
    28741 cgtttgagct ggcgatggat gtgaaggaag agttgttggg atagtgtgtc ttaacgcggg
    28801 aagccttatc cgagctggca acgctgtcct acgtaggcct gataagcgaa gcgcatcagg
    28861 cattgtgtag gcagcagaaa tgtcggataa ggcccgctga ttactgatac accggcagca
    28921 aattaaagct cgataagata tggaccagtg cgttgccaac gccaaatacc agaatcagcg
    28981 taatcatcgg cttgccgccc cagacgcgga atttcgggct gccaaagcgt ttacgcgatg
    29041 cgcgggccag cagtgccgga acaattgccg cccagatggt agctgctaaa ccagcataac
    29101 caatggcgta caggaatccg ttcgggaaca gcagaccacc cacaacagga ggggcaaagg
    29161 tcagcaatgc cgttttcaag cggcccacag ccgagtcgtc gaaaccaaac agatctgcca
    29221 gatagtcaaa caaacccagc gttacgccaa ggaacgaact cgctaccgca aagtttgaga
    29281 acacgaccag cagaaggtcc agactacggc tgttcagtac gccgcttaac gcctgtacca
    29341 gcacatcaat attaccgccc ttctcggcga tgccgataaa ctccggacgc gggatgttac
    29401 ccatcgtcgc cagcaaccag atggtataca gcgccagcgc catcagcgta ccgtacacca
    29461 ggcatttcac gatggttttc ggatctttgc cgtaatactt catcaggctc ggcacgttac
    29521 cgtgataacc aaacgatgcc agacagaacg gcagggtcat caacagatac ggtgcataag
    29581 acgcattgct ttcggcgacg ttgaacaatg tcgcaggctg cacatgcccc agcaggctac
    29641 cgaaggtgag gaagaaggta atgactttcg cccccagcac aatcgctgtc atgcgactga
    29701 cggctttagt gctcaaccac accacaaacg ctaccagcaa tgcaaaacca aaacccgccg
    29761 cccatgccgg gacgtttagt gacatctctg cgaaggtgtg atgcagaatc gaaccactgg
    29821 cagaaatata ggcataggtc aggatataga gcacaaaggc aatggaaatg ccgttgacca
    29881 cgttccagcc tttgccgagc aggtctttgg tgatggtgtc aaaactcgaa ccgattctgt
    29941 aattcaggtt agcctccaga atcatcaagc cggaatgcag catacagaac caggtgaaga
    30001 tcagcgccgc cattgaccag aaaaaccacg ccccggacat gaccactggc agagaaaaca
    30061 tccctgcgcc aataatggtt ccgccgataa tcaccacgcc gccaagcagc gacggtgacg
    30121 tttgggtggt ggttagtgtt gccatgaggg cttctctcca gtgaaaaata gtgcgactgc
    30181 gttgttatgt attgcactgt accagtacac gagtacaaaa gacagaaaaa aagccccgat
    30241 ggttcaaatc ggggctgtat atattatttt acagactgtg ttcgctgttc agcgatgatt
    30301 acgcatcgcc accgaaacga cgacgaccgg tagagtcatc acgacgcggt gcgcggcctt
    30361 cacgacgttc gccgctaaaa cgacgaccat caccacgacc accttcacgg cgttcaccgc
    30421 tgaagttacg accgccctct cgacgctcgc cactaaaacc acgaccaccg ccacgacgct
    30481 caccgccagt atgcggctgt gcatcgccca gtaactgcat attcatcggc ttgttcagaa
    30541 tgcgagtacg cgtaaagtgt tgcagcactt cacccggcat acctttcggc agttcgatgg
    30601 tggagtgaga agcaaacagc ttgatgttac cgatgtaacg gctgctgatg tcgccttcgt
    30661 tagcaatcgc gccaacgata tgacgaactt caacaccatc atcgcggccc acttcaatgc
    30721 ggtacagctg catatcgcca acatcacgac gttcacgacg cggacgatct tcacggtcac
    30781 cacgcgggcc acggtcgtta cgatcgcgcg gaccacggtc atcacggtca cggaattcac
    30841 gtttcggacg catcggcgca tctggcggta cgatcagagt acgttcaccc tgtgccattt
    30901 tcagcagtgc cgcagccaga gtttcgagat ccagctcttc accttctgca gtcggctgaa
    30961 ttttgctcag cagtgcgcgg tattgatcca gatcgctgct ttccagctgc tgctgtactt
    31021 tagcggcgaa tttttccaga cggcgtttgc ccagcagttc tgcgttcggc agttctactt
    31081 ccggaatagt cagcttcata gtacgttcaa tgttgcgcag cagacgacgc tcgcggttct
    31141 caacgaacag cagcgcgcgg ccagcacgac ccgcacgacc ggtacgaccg atacggtgaa
    31201 cgtaagactc agaatccatc gggatatcgt agttaactac caggctgata cgctcaacgt
    31261 ccaggccacg ggctgcaacg tcggtcgcaa tcaggatgtc cagacgacca tctttcaggc
    31321 gttccagtgt ctgctcacgc agcgcctggt tcatgtcacc gttcagcgcg gcgctgttgt
    31381 agccgttacg ctcaagagct tcagccactt ccagagtcgc atttttggta cgaacgaaga
    31441 taatcgccgc atcaaaatct tccgcttcca ggaaacgtac cagtgcttcg tttttacgca
    31501 taccccagac agtccagtag ctctggctga tgtcaggacg ggtagtcacg ctggactgaa
    31561 tgcgcacttc ctgcggctct ttcataaagc ggcgggtaat gcgacgaatc gcttccggca
    31621 tggttgcaga gaacagagcg gtctgatgac cttccgggat ctgcgccata atggtttcaa
    31681 cgtcttcgat gaagcccatg cgcagcattt cgtcagcttc atccagaacc agaccgctca
    31741 gtttagagag gtccagagtg ccacgtttca ggtggtccag cagacggccc ggagtaccga
    31801 caacgatctg cggcccctga cgcagggcgc gtaattgcac gtcataacgc tggccgccgt
    31861 acagggcaac cacatttacg ccgcgcatgt gtttagagaa atccgtcatt gcttcagcaa
    31921 cctgtaccgc cagttcgcgg gtcggtgcca gcaccagaat ctgtggtgct ttcagctcag
    31981 gatcaagatt ctgcagcaga ggtaaagaga atgctgcagt ttttccgctc cccgtctggg
    32041 ccatacccag aacgtcgcgg ccattcagca gatgtggaat acactctgcc tgaattggag
    32101 atggtttttc gtaacccaga tcgttaaggg cttcaaggat aggagccttc aggcccagat
    32161 ctgcaaaagt ggtttcgaat tcagccatgt agtacgtgtg cctcaaaatt aatggcggcc
    32221 agtctacata actcatcatg aaattgatca gcaattttca ttgaaaagtg tgaaccggct
    32281 caaagtaggt gtattaacga acaacaacgc cctcacccgt taaggtgatg gcaatcaaaa
    32341 aagattacgg gctgatgtgt acgtcagcta ttgctggtcc gattctgcca ggtcatcttg
    32401 gtcctggccc aggagcgata attccaacaa tgcgtatcgg tgctcaacaa agttatgaac
    32461 gttgttggca accgccagtt tgaacagtgc cgtggcgctg tccaaatccc ccagacttag
    32521 gtagtactta cctaaataga agttggtttc actgagatgc tcagcgagcg aggtgttatc
    32581 cgttgcgtcc gccttgagcc tttccattaa cgtttgttcg ctaatgttgc ccaggtagaa
    32641 ctcgacaatg ttccatcccc actgttcctt atccgatttt tcgaagtgct gtttcaacac
    32701 ttctttagcc tgcttctcat cgagcttctg ctcggcgaga taaagccaca gactacggaa
    32761 aggatcattg ggatcgtctt gataaaacgc cagcagatca tcttgcgcta acttgtcacg
    32821 accgccgtaa tataatgcga tcccgcgatt caagtgcgcg tagttgtaag ttggatcaag
    32881 ctcaagtaca gaatcaaacg cttcataggc agcatcaaaa ttgcctgcct gcgttaaata
    32941 tatgcctaag taattgaata cttcaggcat atccggtcgg attgccagcg cttgcgaaaa
    33001 atcgttacgc gctaatgccc tcagaccgag actatcatac aacactccgc gctcatataa
    33061 aagctgtgcg cgttcgtcat cggttaaagc ccgactggca aggatttgtt ccatacgtgc
    33121 cagaatcact tcctgctgta aagtcggttg caatggtacc gcgaggactt cacttttacg
    33181 ccaggaagta ttactgcatc ctgcaagcgt aagtgctgtc gcaacgaaac accagcgcaa
    33241 aaaaggcttc atttcccact cccgaagacc acggttgaat gaacgtcctg ttcccggttg
    33301 ctaacaaggc gtcctgcccg gttaaaagcc ccccgccgca gcggagggca aatggcaacc
    33361 ttactcgccc tgttcagcag ccggagcttc cggtgctgca gcaggttgag actgctcagt
    33421 cgcttcttta atgctcagac ggatacggcc ctggcgatca acttccagaa ctttcaccgg
    33481 tacttcctga cccatctgca ggtaatcggt cactttctca acgcgtttgt cagcgatttg
    33541 agagatgtgt accagacctt ctttaccgcc gccgatggca acaaatgcgc caaagtcaac
    33601 gatacgggtc actttaccag tgtagacgcg gcccacttcg atttctgcag tgatctcttc
    33661 gatacgacga atagcgtgtt tcgctttctc gccatcggtc gctgcgatct tcacagtacc
    33721 gtcatcttcg atttcgatgg tggtgccagt ttcttcggtc agggcacgga tcacagagcc
    33781 gcctttaccg ataacatctt tgatcttgtc cgggttgatc ttgatggtat ggatacgcgg
    33841 agcgaactca gagatatcgc cacgtggcgc gttgatcgcc tgttccatta cacccaggat
    33901 gtgcagacgc gcacctttag cctggttcag tgcaacctgc atgatctctt tggtgatacc
    33961 ttcaattttg atatccatct gcagtgcaga gataccgtcg cgggaacccg caactttgaa
    34021 gtccatatca cccaggtgat cttcgtcgcc caaaatgtca gacagtacaa cgtagttgtc
    34081 gccttctttc accagaccca ttgcgatacc cgcaacggca gctttgattg gcacacctgc
    34141 gtccatcagc gccagagacg caccgcacac ggaagccata gaagaagaac cgttggattc
    34201 agtgatttca gacacaacac gtacggtgta cgggaatttg tccatatccg gcatgactgc
    34261 cagcacgccg cgcttcgcca gacgaccgtg accaatttca cgacgcttcg gagaaccgac
    34321 catgccggtt tcgcctacgg agtacggagg gaagttgtag tggaacagga aggtatcggt
    34381 acgttcgccc atcagttcat caagaacctg cgcgtcacga gcagtaccca gcgttgcggt
    34441 aaccagcgcc tgcgtttcac cacgggtgaa cagcgcagaa ccgtgagtac gcggcagcac
    34501 gccagtacgc acatccaaac cacggatcat atctttttca cgaccgtcga tacgcggttc
    34561 gcctgccagt acgcggctac gaacaacgtt tttctcgata gcgtgcagaa tttcacccag
    34621 ttcgttttcg tccagggttt cgtcttcagc aagcagcgtc gcgatggttt cagatttgat
    34681 gacatcaacc tgcgcataac gctcttgttt gtcggtgatg cggtaagcat cgctcaggcg
    34741 agcttcagcc agtgcagcaa cgcgcgcgtt cagcgcttcg tttaccggct ccggctgcca
    34801 gtcccaacgt ggtttaccgg cttctttcac cagttcattg atgttctgaa taacaacctg
    34861 ctgttgttca tgaccgaaca ccactgcgcc cagcatttgg tcttcgctca gcagttcagc
    34921 ttcagattca accatcagca cggcagcttc agtaccggca acaaccagat ccagtttgct
    34981 ctctttcagc tcgtcctgtg tcgggttcag tacgtactgg tcattgatgt aacctacgcg
    35041 ggcagcacca atcggaccat tgaacggaat accagacaga gacagtgctg cggaagcacc
    35101 aatcatcgcg acgatatccg ggttaacctg cgggttaaca gaaaccacgg tggcgataac
    35161 ctgaacttcg ttgacgaagc cttccgggaa cagcgggcga atcgggcggt caatcagacg
    35221 cgcgatcagg gtttcgcctt cgcttggacg accttcacga cggaagaagc tacccgggat
    35281 acgaccagca gcgtaggtac gctcctgata gttaacggtc agtgggaaga agtcctgacc
    35341 tggtttggct tttttctggc caacaacggt aacaaatacc gcggtgtcat ccatgctaac
    35401 cataacagcg gcagtagcct gacgagccat catgccggtt tccagagtca cggtgtgttg
    35461 gccgtactgg aatttacgaa cgatcggatt aagcaatgta atatcctttc tctttcttag
    35521 acagtacctt acggcactgg tgttaatacc cgatcttctg cgcatcctcg cgactaatga
    35581 caaccctaac ccagctctat gtgggtaaag cctctcatta gccgcgcgaa cctctgcaac
    35641 ggaagatcat tcatagcaac aatacattag tttccagtga attgctgccg tcagcttgaa
    35701 aaaaggggcc actcaggccc ccttttctga aactcgcaag aattagcgac gcagacccag
    35761 gcgctcgatg agctgggtgt aacgtgctac gtctttacgt ttcaggtagt cgagcagttt
    35821 acgacgctga gaaaccatgc gcagcagacc acgacggctg tggtgatctt ttttgtgctc
    35881 tgcaaagtgg ccctgcaggt ggttgatctg tgcagtcagc agtgctacct gaacttcggt
    35941 agaaccggtg tcgtttgcgt cacgaccaaa ctcagaaacg attttagctg ttgcttcagt
    36001 acttagagac attttaaaac tccaaagtat atagaatgaa aggacgccga tctctaattc
    36061 agcgatccca gtgtacgtta cgcaaagtgt taaacaattt acgcgacgtt aagcggcagt
    36121 attctactcg tagcgacctg ttatcgcaag acggttaaca ttacgccggg tattcaacca
    36181 ccagacgacg aggcgcaacg cggccttcat cgtcaatttc gcccataccg ataaacttgc
    36241 cgttctcacc ttccgtgacg cgaaccagtc cttccagcgg cgcgccagat gtacgaaccg
    36301 ggttaccatt tttgaagtaa acagaagacg ttaacggaag attcaccacc ggatagtccg
    36361 aagctggact gtccattggc atcagtaatg gatcaagtaa ctccgcagct ggaatatcct
    36421 gctgttcggc ttgttcgacc agttcacgca gatgttccag ggtcaccatc cgttcaaccg
    36481 gatatttact taccgccaga cggcgcaggt aaataacatg cgcgccacaa ccgagtttct
    36541 cacccaggtc gtcaatgata gtgcggatat aagtgccttt tgagcagtga atttccagct
    36601 ccagctcatt gccttcatgg cgaataaaca gcaattcata aacggtaatc ggacgcgctt
    36661 cacgcggaac ttcaatgccc tgacgcgcat attcgtacag ttttttgccc tggtatttca
    36721 gtgctgaata catcgatggg atctgttcga tatcgccacg gaaagtatcc agtgccgccg
    36781 ccagttgttc tgcactaaag gttaccggac gctcttcaac gatttgtccg tcggcgtcag
    36841 aagtatcggt acgctgtcca agacgcgcaa tgacccgata gcgtttgtcg gagtccagca
    36901 gatactggga aaacttcgtc gcttccccga ggcaaatcgg caacatgccg gtcgccagcg
    36961 ggtccagcgc accggtatgc ccggcacggt tggcgttata aatccgtttc actttttgca
    37021 gcgcatcgtt gctggacata ccctgaggtt tatccagcaa caaaacgccg ttaatgtcgc
    37081 gaccgcgacg acgaggacga ctcattagtc ctccttgctg tcgtccgggt taacacgacg
    37141 ttcttcgtca tgtttgacca cgctggtcac caggtttgac atgcgcatcc cttcaaccag
    37201 agagttgtcg tagaagaagg tcagttccgg cacgatacgc aggcgcatcg ctttccccag
    37261 caggctgcgg atgaaaccag aagcttcttg caacgctttg atgcccgctt taaccgcgtc
    37321 ttcatctttg tcgttgagga acgtcacata tactttggca tacgccaggt cgcgagacat
    37381 ttcgacaccg gaaacggtgg tcatcatgcc caggcgagga tctttaattt cacgctgcag
    37441 gatgagagcg atctcttttt gcatttcctg cgctacgcgc tgcgggcgac caaattcttt
    37501 cgccataata aattctcctg acaaaaaagg ggctgttagc ccctttttaa aattaatttc
    37561 aggtggaagg gctgttcacg ttggtctgat aagacgcgcc agcgtcacat caggcaatcc
    37621 atgccggatg cggcgtaaac gccttatccc gcatggaacc ctaaaaacct taagcgatgg
    37681 tacgttggat ctcgatgatt tcgaatactt cgatcacatc gccagtgcgg acgtcgttgt
    37741 agttcttaac gccgatacca cattccatac cgttacggac ttcgttaacg tcatctttga
    37801 agcggcgcag ggactccagc tcgccttcgt agataaccac gttgtcacgc agaacgcgga
    37861 tcgggttgtg acgtttaacc acaccttcgg taaccataca gcctgcgatg gcaccaaatt
    37921 tcggcgattt gaacacgtca cgaacttccg ccagaccgat aatctgctgt ttcagttccg
    37981 gagacagcat accgctcatc gccgctttca cttcgtcgat caggttatag atgacggagt
    38041 agtaacgcag atctaggctt tccgcttcaa tcactttacg tgcagaggca tcagcacgta
    38101 cgttgaagcc aaccaggatg gcgttggacg ccgcagccag ggtggcgtcg gtttcggtga
    38161 taccacctac gccagaaccg atgatcttca ctttaacttc gtcagtagac agtttcagca
    38221 aggagtcgga gatcgcttcc acagaaccct gtacgtctgc cttcaggacg atattcactt
    38281 cgtgaacttc gccttcagtc atgttggcaa acatgttctc gagtttagat ttctgctgac
    38341 gcgccagttt aacttcgcgg aatttaccct gacgatagag tgcaacttca cgcgctttct
    38401 tctcgtcacg tacaacggta acttcatcac ccgcagccgg aacaccggac aagccgagga
    38461 tttccaccgg aatggacgga cccgcttcca gcacttcctg acccagttcg ttacgcatcg
    38521 cacgaacacg accgtattcg aagccacaca gaacaatatc gcccttgtgc agagtacctt
    38581 cacgtaccag aacggtagca accggaccac gacctttatc gaggaaggat tcgataaccg
    38641 caccgctcgc cataccttta cgtaccgctt tcagctccag aacttccgcc tgcagcagga
    38701 tagcgtccag cagttcatcg ataccggtac ccgctttcgc agatacgtgt acgaactggc
    38761 tttcaccgcc ccactcttcc ggcaggatgc cgtactggga gagttcgttc ttaacgcgat
    38821 ccggatcggc ttctggttta tcgatcttgt tcactgcaac caccaccggt acctgcgccg
    38881 ctttcgcgtg ctggattgct tcgatggtct gcggcattac gccgtcgtcg gcagcaacaa
    38941 ccaggactac gatgtcagtt gcctgcgcac cacgtgcacg cattgaagta aacgcggcgt
    39001 gccccggggt gtccaggaag gtgatcatgc cgttttcagt ttcaacgtgg tatgcaccaa
    39061 tgtgctgggt aatgccgccc gcttcgccag aggccacttt cgttgaacga atgtagtcca
    39121 gcagagaggt tttaccgtgg tcaacgtgac ccatgatggt cacaaccgga gcgcgcggtt
    39181 cagccgcagc ccccgtgtca cggtcgctca ttaccgcctc ttccagctcg ttttcacgac
    39241 gcaggataac tttgtggccc atctcttcag caaccagctg tgcggtttcc tgatcgataa
    39301 cctggttgat ggttgccatt gcgcccagtt tcatcatcgc tttgatgacc tgagagcctt
    39361 taaccgccat cttgttcgcc agttcgccaa cggtgatagt ttcgccgatc acaacgtcac
    39421 ggttaacggc ctgagcaggc ttctggaagc cttgctgcag cgaagaacct ttacgttttc
    39481 cgcccttacc gccacgtact gctgcgcgtg cttcttcacg atcagctttt gattcagcgt
    39541 gtttgttgcc tttcttcgga cgcgctgctt tcgcgttacg accacggcca cggccgcctt
    39601 cgacttcacg atcgctttcg tcttctgcct ggcgagcatg ttgagaagta gtgacgtgat
    39661 aatcgctgga atcttcagtc ggttccgcgt tatcagtcca tttgttttct tccgccatac
    39721 gacgtgcttc ttcagcaacg cgacgtgctt cttcttcgag tttacgacgc gcttcttctt
    39781 cggctttacg cttgagctct gcagcttcct gctcacggcg ggctttttca gcctgggcgt
    39841 ttttagtcat atcgtcttgt tgattgctca ctttgtcttt ttccgcagct tcacgtttcg
    39901 cttgttcagc agcttcacgc ttagcttgtt ctgcggcctc acgttcagct ttttgttgcg
    39961 cctcgcgttt agccgattct tctgcctcac gacgggcttg ctcttccgct tcacgctgcg
    40021 cttgctcttc cgctgcaagg cgttcagcct cttgcggatc gcgtttcaca aaggtgcgtt
    40081 tcttgcggac ttcgatttgt accgatttgc tttttccacc ggtaccagga atgttaaggg
    40141 tgctgcgtgt tttacgttgc agcgtcaatt tgtccgggcc tgaatttttc tgattcaggt
    40201 ggtcaatcaa agtctgtttc tcttgtgcag acacagagtc gtcagcagac ttccggatac
    40261 ctgcatcagc aaattgctgt accaggcgtt ccacggaggt ctgtcgctct gcggccagcg
    40321 ttttaatcgt tacatctgtc atgctgttcc ttcctgctac agtttattac gcttcgtcac
    40381 cgaaccagca aatattacgg gcagccataa tcagtgctcc ggctttttcg tcggtcaacc
    40441 cttcgatatc agccagatca tcaatgccct gttcggcgag atcttccagc gtacaaacgc
    40501 cacgggcggc cagtttgaat gccaaatcac gatctacccc ttcaaggttc agcagatcgt
    40561 cagccggttt gttatcaccg aggctttctt cctgggcctg tgcaatggtg gccagtgcat
    40621 ttttagcacg ctcgcgcagt gcttcaacgg tcggctcatc aaggccttcg atttccaaca
    40681 gctctttcat cggcacatag gccagttctt ccagcgtcga gaagccttct tctaccagaa
    40741 cagtcgcgaa gtcttcgtcg atgtcgagat atttggtgaa ggtgtcgatc gctgcgtgcg
    40801 cttccgcctg atgcttagcc tgcaggtcgt caacggtcat cacgttaagt tcccaaccgc
    40861 tcagctgcga agccagacgc acgttctgac cgttacggcc aatcgcctgc gccaggttac
    40921 cggcttcaac ggcgatatcc atggtgtgtt tatcttcatc caccacgata gaagcaacgt
    40981 ctgccggtgc catagcgtta atcacgaact gcgccgggtt atcatcccac aggacgatat
    41041 cgatacgctc gccgcccagt tcagtagaaa ccgcctgaac acgcgcgcca cgcataccta
    41101 cgcaagcacc taccggatcg atacgtttat cgttggtttt caccgcgatt ttcgcacgag
    41161 aacccgggtc gcgagccgct gctttaattt caatcacttc ttcgccgatt tctggcactt
    41221 caatacggaa cagttcgatc agcatttccg gcttggagcg agtgacgaac agttgcgcgc
    41281 cacgcgcttc cgggcgaacg gaataaagca cgccacgaac gcggtcgcca gggcggaagt
    41341 tttcacgcgg cagcatatct tcgcgcagga tcacggcttc agcgttgttg cccagatcca
    41401 gagagatgtt gtcgcggttt acttttttca ccacgccggt gatgatttca ccttcgtgtt
    41461 cacggaactg atcaaccacc atcgcacgtt cggcttcacg cactttctgc acgataacct
    41521 gttttgccgt ctgggtggtg atacggtcaa aggtaacaga ctcaatctga tcttcaacgt
    41581 aatcgcccag gttcaggctt tcatcttcat aacgtgcggc ttcaagggtg atttccttgg
    41641 tcggctgggt gacttcatca acaactaacc agcgacggaa ggtgtcaaaa tcaccgcttt
    41701 tgcgatcgat ctgtacgcgg acgtcgatct cttgttcata ttttttcttt gttgctgtcg
    41761 ccagcgcgct ttccaatgct tcgaaaatct tctcgcgagg tagcgccttt tcattggata
    41821 cggcttcaac tacagccaaa atttctttgt tcatcgcggg cttttcacct catccagact
    41881 attaaaagtg gggaaccagg ttcgccttct ggatattact cagcgcgaac acttcatctt
    41941 taccttcgac ggtaactgtg atcatttcac cgtctaccgc tttgataacg ccctgccatt
    42001 tacgacggtt ttgtaccgcc atacggagaa ccagagtcac ctcttctccg acaaaacggg
    42061 cgtagtgttc agccgtgaac agtgggcgat cgagacccgg tgaggagact tccaggttgt
    42121 aagcaacggt gatgggatct tcaacatcca gcacagcact tacctggtgg ctcacatcag
    42181 cacaatcatc aacattgatg ccatcttcac tatcaatata gatgcgcagt gtggatgtgc
    42241 gaccgcgaat aaattcgatg ccaaccagtt caaaacccag ggcctcaact ggcgcagtaa
    42301 tcatctctgt taatttttgc tctaatgtgg acaagcccac ccccaagaca taaaaaaagg
    42361 gcctaaagcc cagttattct gtagtcagat aacaaaaaac cccgataaat cggggcttta
    42421 tataactgaa ccctataacc gcaactgcgg tctggagcac tttccagaag gattttttca
    42481 aatcccacta cgaaggccga agtcttcaca gtatatttga aaaaggactc taagggaaag
    42541 tggttgcggg ggccggattt gaaccgacga ccttcgggtt atgagcccga cgagctacca
    42601 ggctgctcca ccccgcgcct gaaacgtggc aaattctact cgttttgggt aaaaaatgca
    42661 aatactgctg ggatttggtg taccgagacg ggacgtaaaa tctgcaggca ttatagtgat
    42721 ccatgccaca ttttgtcaac gtttattgct aatcacgtga atgaatatcc agttcacttt
    42781 catttgttga atacttttgc cttctcctga tcccccttaa gcgcattatt ttacaaaaaa
    42841 cacactaaac tcttcctgtc tctgataaaa gatgattaaa tgaaaactca tttattttgc
    42901 ataaaaattc agtgagagcg gaaatccagg ctcatcatca gttaattaag cagggtgtta
    42961 ttttatgacg acgattctca agcatctccc agtaggtcaa cgtattggta tcgctttttc
    43021 cggcggtctg gacaccagtg ccgcactgct gtggatgcga caaaagggag cggttcctta
    43081 tgcatatact gcaaacctgg gccagccaga cgaagaggat tatgatgcga tccctcgtcg
    43141 tgccatggaa tacggcgcgg agaacgcacg tctgatcgac tgccgcaaac aactggtggc
    43201 cgaaggtatt gccgctattc agtgtggcgc atttcataac accaccggtg gcctgaccta
    43261 tttcaacacg accccgctgg gccgcgccgt gactggcacc atgctggttg ctgctatgaa
    43321 agaagatggc gtgaatatct ggggtgacgg cagcacctat aaaggaaacg atatcgaacg
    43381 tttctatcgt tacggtctgc tgaccaatgc tgaactgcag atttacaaac cgtggcttga
    43441 tactgacttt attgatgaac tgggcggtcg tcatgaaatg tctgaattta tgattgcctg
    43501 cggtttcgac tacaaaatgt ctgtcgaaaa agcctactcc acagactcca acatgcttgg
    43561 tgcaacgcat gaagcgaagg atctggaata cctcaactcc agcgtcaaaa tcgtcaaccc
    43621 gattatgggc gtgaaattct gggatgagag cgtgaagatc ccggcagaag aagtcacagt
    43681 acgctttgag caaggtcatc cggtggcgtt gaacggtaaa acctttagcg acgacgtaga
    43741 aatgatgctg gaagctaacc gcatcggcgg tcgtcacggc ctgggcatga gcgaccagat
    43801 tgaaaaccgt atcatcgaag cgaaaagccg tggtatttac gaagctccgg ggatggcact
    43861 gctgcacatt gcgtatgaac gcctgttgac cggtattcac aacgaagaca ccattgagca
    43921 gtatcacgcg catggtcgtc agttgggccg tctgctgtac caggggcgtt ggtttgactc
    43981 ccaggcgctg atgctgcgtg actctctgca acgctgggtt gccagccaga tcactggtga
    44041 agttaccctg gagctgcgcc gtgggaacga ttattcaatc ctgaataccg tctcagagaa
    44101 cctgacctac aagccagagc gtctgacgat ggaaaaaggc gactcggtgt tctcgccaga
    44161 tgatcgtatt ggtcaattga ccatgcgtaa cctggatatc accgataccc gagagaaact
    44221 ttttggttat gccaaaactg gcctgctctc ctcctctgcc acttcaggcg tgccgcaggt
    44281 ggagaatctg gaaaacaaag gccagtaatt cgacatcaac cctgcccttc aggggtgggg
    44341 ttacattgct tatcctctgt aaactccttt ctgagcatca cataatcgaa aacatcatgg
    44401 tttgcatcca caacccgtga gtctccttta tagtgtgcaa tcacttcctc aagcgtttgc
    44461 ggctgttccg gctttgttac ccccatccac gcattaataa ggtaattatt gtaagctgtc
    44521 gaaaagatgt tgttttccgt tgtgcgatcc acaccatccc caagaacggg gctataccag
    44581 ataaacatcg gaacatgata tgcctgctgg ctggcctccc tgcctccatg aaaatagacg
    44641 ttcttcttcg tagggtcacg ttccaggccg tggtcggcaa aatacatgac cgaggcgcgg
    44701 cgatctttta ataattcaaa aacctgacct agcaaactat ctgtgtaatg aatggagttg
    44761 tcatagcagg catcctgatc gtcctgcggt tgaaacacgg cactggattg cggataggcg
    44821 ctacaagccg gttcatggct tccatttaaa tgaagaacaa tcagtttctt ttgctgtgta
    44881 ttttgctgta atgcttgcga taaatgcggc aacaacaatt catcaaatcc tctgacataa
    44941 actgtttcca tggcgcgcat ggcgatactg gtaactgctg taccattctg ccgaaaagcg
    45001 gattgcgaac ttagccagaa agtctgaaat cctgcttgat tagccatatt aataatgttg
    45061 tcggggtaat tatgaatgtc atggctcaaa acagaatcag cagttaaaga aaggggaacc
    45121 gacagcgcag tgtaaggtgc accgcttatt gcttgattaa acagtttgat ctgttttctt
    45181 tgtgcttcga cttgcggtgt cgtagagcgt gtatatccat acaaagacat attgtcgaca
    45241 cgtacagact cccccacaat caacacgtag gtatcaatac ctgtatccct gactgataat
    45301 tgaaaatacg gcactgtgtt tgcaattgag agtaatcttt gatgctcttt cgctgctaaa
    45361 gcaaaataat tgagattgaa aaacggcgta taggtagcaa atcgcgacgc tagtatatat
    45421 ggactgaacg catttttatt tttggcatct ttataagcaa attgacaagc ggaaaataaa
    45481 cttcccgaaa tgacaatcag caataatatt cctgtcactt tttttgtcgg caaggaaaca
    45541 tcatatttta ttattacagc caaaaaaaga agggataaaa aggcaaaagc acatagataa
    45601 gggctataca ttcccagcat tttgacaact tcatccggat cactctgcag cacactaatc
    45661 gcaaatccat cattgaaagt tgtaccaaag gtacaccatg aatagaggct aatactcatg
    45721 tctgccgcag tcagtaacac aaatggtgcg gcaacaagca ggcgaagcgg aagggatgat
    45781 atacgcttaa caaccaggat ggtcagaacg aagaacagca atcgctgaac catatgcgct
    45841 ccggaagagg cgactaagtt cgtaatacca aaaacaataa cactaagata caacaaccaa
    45901 tgagatttaa aacttctagc aaatttgttg aatactgtca tccagaaatc caagttcaca
    45961 accaatataa acaatta
//
LOCUS       DAPGOF010000015        45637 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.015, whole genome shotgun sequence.
ACCESSION   DAPGOF010000015 DAPGOF010000000
VERSION     DAPGOF010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45637
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.015"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            172..798
                     /locus_tag="RHV13_000734"
     CDS             172..798
                     /locus_tag="RHV13_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HDV1240292.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            819..2921
                     /locus_tag="RHV13_000735"
     CDS             819..2921
                     /locus_tag="RHV13_000735"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HDV1240293.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            2934..3572
                     /locus_tag="RHV13_000736"
     CDS             2934..3572
                     /locus_tag="RHV13_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HDV1240294.1"
                     /translation="MNHRDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            3636..4304
                     /locus_tag="RHV13_000737"
     CDS             3636..4304
                     /locus_tag="RHV13_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDV1240295.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4301..5086
                     /locus_tag="RHV13_000738"
     CDS             4301..5086
                     /locus_tag="RHV13_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HDV1240296.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKIYLYSKAVRLWHWSNALLFVLLLASGLINHFALVGVT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGGLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQTHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5090..5902
                     /locus_tag="RHV13_000739"
     CDS             5090..5902
                     /locus_tag="RHV13_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1240297.1"
                     /translation="MSITQADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGERPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHHFVEC"
     gene            complement(5914..7518)
                     /locus_tag="RHV13_000740"
     CDS             complement(5914..7518)
                     /locus_tag="RHV13_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HDV1240298.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARKQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEQATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYAILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLNTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(7644..7949)
                     /locus_tag="RHV13_000741"
     CDS             complement(7644..7949)
                     /locus_tag="RHV13_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HDV1240299.1"
                     /translation="MATLLQLHFSFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(8057..8133)
                     /locus_tag="RHV13_000742"
     tRNA            complement(8057..8133)
                     /locus_tag="RHV13_000742"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8097..8099),aa:Val,seq:gac)
     gene            complement(8138..8214)
                     /locus_tag="RHV13_000743"
     tRNA            complement(8138..8214)
                     /locus_tag="RHV13_000743"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8178..8180),aa:Val,seq:gac)
     gene            8522..9778
                     /locus_tag="RHV13_000744"
     CDS             8522..9778
                     /locus_tag="RHV13_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HDV1240300.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGAMQLTEGELIVEAGGRAENT
                     VVTGAGWLKVTTGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDKGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKANDTLLAGGTMNNLGGEDSDTIVENGAIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFSIGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(9819..11192)
                     /gene="mdtK"
                     /locus_tag="RHV13_000745"
     CDS             complement(9819..11192)
                     /gene="mdtK"
                     /locus_tag="RHV13_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HDV1240301.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSAI
                     ILQRASR"
     gene            11407..12048
                     /gene="ribE"
                     /locus_tag="RHV13_000746"
     CDS             11407..12048
                     /gene="ribE"
                     /locus_tag="RHV13_000746"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HDV1240302.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(12088..13236)
                     /gene="cfa"
                     /locus_tag="RHV13_000747"
     CDS             complement(12088..13236)
                     /gene="cfa"
                     /locus_tag="RHV13_000747"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HDV1240303.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13527..14738)
                     /locus_tag="RHV13_000748"
     CDS             complement(13527..14738)
                     /locus_tag="RHV13_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HDV1240304.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            14851..15783
                     /locus_tag="RHV13_000749"
     CDS             14851..15783
                     /locus_tag="RHV13_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV1240305.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(15780..16805)
                     /gene="purR"
                     /locus_tag="RHV13_000750"
     CDS             complement(15780..16805)
                     /gene="purR"
                     /locus_tag="RHV13_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HDV1240306.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17104..17193
                     /gene="ynhF"
                     /locus_tag="RHV13_000751"
     CDS             17104..17193
                     /gene="ynhF"
                     /locus_tag="RHV13_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HDV1240307.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            17359..18528
                     /locus_tag="RHV13_000752"
     CDS             17359..18528
                     /locus_tag="RHV13_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1240308.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(18674..19255)
                     /gene="sodB"
                     /locus_tag="RHV13_000753"
     CDS             complement(18674..19255)
                     /gene="sodB"
                     /locus_tag="RHV13_000753"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HDV1240309.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19383..20210)
                     /gene="mepH"
                     /locus_tag="RHV13_000754"
     CDS             complement(19383..20210)
                     /gene="mepH"
                     /locus_tag="RHV13_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HDV1240310.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHTVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20545..20892
                     /gene="grxD"
                     /locus_tag="RHV13_000755"
     CDS             20545..20892
                     /gene="grxD"
                     /locus_tag="RHV13_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HDV1240311.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(20943..25559)
                     /locus_tag="RHV13_000756"
     CDS             complement(20943..25559)
                     /locus_tag="RHV13_000756"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDV1240312.1"
                     /translation="MADNPNPSSPLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDQLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLQGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAADAAHFE
                     STSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKLGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQHTVAAAAMDALQVDEWYSRARRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTISPWLAEENTIANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVVSYLE
                     NLLAVKRIFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLLLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDPSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPRVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQAAQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(25652..26299)
                     /gene="rnt"
                     /locus_tag="RHV13_000757"
     CDS             complement(25652..26299)
                     /gene="rnt"
                     /locus_tag="RHV13_000757"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HDV1240313.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(26402..26809)
                     /gene="gloA"
                     /locus_tag="RHV13_000758"
     CDS             complement(26402..26809)
                     /gene="gloA"
                     /locus_tag="RHV13_000758"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HDV1240314.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(26890..27987)
                     /gene="nemA"
                     /locus_tag="RHV13_000759"
     CDS             complement(26890..27987)
                     /gene="nemA"
                     /locus_tag="RHV13_000759"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HDV1240315.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(28024..28623)
                     /gene="nemR"
                     /locus_tag="RHV13_000760"
     CDS             complement(28024..28623)
                     /gene="nemR"
                     /locus_tag="RHV13_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HDV1240316.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNSEPLENALAHVKTIIATPAV"
     gene            28726..28965
                     /locus_tag="RHV13_000761"
     CDS             28726..28965
                     /locus_tag="RHV13_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HDV1240317.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            29014..29910
                     /locus_tag="RHV13_000762"
     CDS             29014..29910
                     /locus_tag="RHV13_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HDV1240318.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            29991..30512
                     /gene="sodC"
                     /locus_tag="RHV13_000763"
     CDS             29991..30512
                     /gene="sodC"
                     /locus_tag="RHV13_000763"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HDV1240319.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(30513..32525)
                     /locus_tag="RHV13_000764"
     CDS             complement(30513..32525)
                     /locus_tag="RHV13_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDV1240320.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDVYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHTRLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(32525..33382)
                     /locus_tag="RHV13_000765"
     CDS             complement(32525..33382)
                     /locus_tag="RHV13_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDV1240321.1"
                     /translation="MSIKTIKYFSTIIVAIVSVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAKLNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFTLDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(33385..33621)
                     /locus_tag="RHV13_000766"
     CDS             complement(33385..33621)
                     /locus_tag="RHV13_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HDV1240322.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            33822..34256
                     /gene="slyA"
                     /locus_tag="RHV13_000767"
     CDS             33822..34256
                     /gene="slyA"
                     /locus_tag="RHV13_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HDV1240323.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(34302..34769)
                     /gene="slyB"
                     /locus_tag="RHV13_000768"
     CDS             complement(34302..34769)
                     /gene="slyB"
                     /locus_tag="RHV13_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HDV1240324.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            35042..36151
                     /gene="anmK"
                     /locus_tag="RHV13_000769"
     CDS             35042..36151
                     /gene="anmK"
                     /locus_tag="RHV13_000769"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HDV1240325.1"
                     /translation="MKSGRFIGIMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            36249..36578
                     /gene="mliC"
                     /locus_tag="RHV13_000770"
     CDS             36249..36578
                     /gene="mliC"
                     /locus_tag="RHV13_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HDV1240326.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            36637..37293
                     /gene="pdxH"
                     /locus_tag="RHV13_000771"
     CDS             36637..37293
                     /gene="pdxH"
                     /locus_tag="RHV13_000771"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HDV1240327.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            37422..38696
                     /gene="tyrS"
                     /locus_tag="RHV13_000772"
     CDS             37422..38696
                     /gene="tyrS"
                     /locus_tag="RHV13_000772"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDV1240328.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            38758..39618
                     /gene="pdxY"
                     /locus_tag="RHV13_000773"
     CDS             38758..39618
                     /gene="pdxY"
                     /locus_tag="RHV13_000773"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HDV1240329.1"
                     /translation="MKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNHT
                     QYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKAA
                     NPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHPVNNV
                     EEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQP
                     VGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIAK
                     PEHYFSATKL"
     gene            complement(39662..40267)
                     /gene="gstA"
                     /locus_tag="RHV13_000774"
     CDS             complement(39662..40267)
                     /gene="gstA"
                     /locus_tag="RHV13_000774"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HDV1240330.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(40373..41875)
                     /gene="dtpA"
                     /locus_tag="RHV13_000775"
     CDS             complement(40373..41875)
                     /gene="dtpA"
                     /locus_tag="RHV13_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HDV1240331.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHTILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(42486..43121)
                     /gene="nth"
                     /locus_tag="RHV13_000776"
     CDS             complement(42486..43121)
                     /gene="nth"
                     /locus_tag="RHV13_000776"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDV1240332.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLDQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(43121..43816)
                     /gene="rsxE"
                     /locus_tag="RHV13_000777"
     CDS             complement(43121..43816)
                     /gene="rsxE"
                     /locus_tag="RHV13_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HDV1240333.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWVKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(43820..44440)
                     /gene="rsxG"
                     /locus_tag="RHV13_000778"
     CDS             complement(43820..44440)
                     /gene="rsxG"
                     /locus_tag="RHV13_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HDV1240334.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(44444..45502)
                     /gene="rsxD"
                     /locus_tag="RHV13_000779"
     CDS             complement(44444..45502)
                     /gene="rsxD"
                     /locus_tag="RHV13_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HDV1240335.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLTLCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
BASE COUNT        11492 a        11279 c        11910 g        10956 t
ORIGIN      
        1 tttctcctaa ccataaagga ttagtttatc ggcgttaaat cttttctcat acactatcgg
       61 gttagggaaa atatctggtg ttggggacag agtcagcagc gtattagttg aatgcttaat
      121 atcaggctgc tgaattgtca gcactgtgtt tgcgaagagg atgcgaaata aatgaacccg
      181 gttgatcgtc cactattaga tattggctta acgcgattag aattcttgcg tatatcagga
      241 aaaggccttg ctggtctaac aattgcccct gcgttactgt cacttttggg ttgcaaacag
      301 gaagatattg atagcggcac agtagggttg ataaatacac ccaaaggggt gctggtgacc
      361 cagcgagcgc gatgcacggg ttgccaccgc tgtgaaatct cttgtaccaa cttcaacgat
      421 ggctcagtag ggacattctt ctcccgtatc aaaatccacc gcaattattt ctttggcgac
      481 aacggggttg gctctggcgg cggcctgtat ggcgatctca actataccgc ggacacctgc
      541 cgtcaatgca aagagccgca atgcatgaac gtctgcccga ttggtgcgat tacctggcag
      601 cagaaagaag gctgtattac cgtcgatcat aaacgttgta ttggctgtag cgcctgtact
      661 acggcatgcc cgtggatgat ggccaccgta aataccgaaa gtaaaaaatc ctcgaaatgt
      721 gtgttatgcg gtgaatgcgc aaacgcctgc ccaacagggg cgttaaaaat tatcgagtgg
      781 aaagatatta ctgtgtgaat cttgcaaagg aaaaaatcat ggctaacggt tggacaggta
      841 atatattgag agtcaatctt acgacaggaa atattaccct cgaagattcc agtaagttta
      901 aaagttttgt cggtggcatg ggcttcggct acaaaattat gtatgacgaa gtaccgccag
      961 gcacgaaacc tttcgatgaa gcgaataaat tagtctttgc taccggccca ttaactggat
     1021 ctggtgcccc ctgtagttct cgcgtaaata tcacctcact ttctactttt accaaaggaa
     1081 atttagtcgt cgatgcccat atgggtggct tttttgcagc gcaaatgaaa ttcgctggat
     1141 acgacgtcat tattatcgaa gggaaggcga aatcaccggt atggctgaag attaaagatg
     1201 acaaagttag cctggaaaaa gcagatttct tatggggaaa agggacgcgc gcaacgacgg
     1261 aagaaatttg tcgcttgacc agtccggaaa cctgtgtggc ggctattggt caggctgggg
     1321 aaaaccttgt tcctctctct ggcatgttga atagccgtaa ccacagcggc ggtgcgggaa
     1381 caggcgcaat aatgggttcg aaaaacctga aagcgattgc ggttgaaggg acgaaagggg
     1441 tcaacattgc cgatcgtcag gagatgaagc gtctcaatga ttacatgatg actgaactta
     1501 ttggtgcgaa taacaaccat gtcgtgccaa gtacgccaca atcgtgggca gagtattcag
     1561 atcccaagtc acgctggaca gcacgtaaag ggctgttttg gggcgcggct gaaggcggtc
     1621 caattgaaac gggtgaaatt ccgccaggca atcagaatac ggtcggcttt cgtacctata
     1681 aatccgtttt tgacttagga ccggcggcag agaaatacac agtaaaaatg agcggctgcc
     1741 actcttgccc gatccgttgt atgacccaaa tgaatattcc tcgggtgaaa gagtttggcg
     1801 tgcccagcac aggtggtaac acttgtgtag caaactttgt ccataccacc atcttcccga
     1861 acgggccgaa agattttgaa gataaagacg atggtcgtgt gattggtaac ctggttggtc
     1921 tgaatttgtt cgatgactac ggcctatggt gtaactacgg gcagttgcat cgcgacttta
     1981 cttattgtta tagcaaaggt gtgttcaagc gtgttctgcc tgctgaagag tatgcggaaa
     2041 ttcgctggga tcaactggaa gcgggtgacg ttaacttcat taaagatttt tactaccgtc
     2101 tggcgcatcg tgtgggggag ctgagtcacc tggctgatgg ttcatatgcc atcgcagaac
     2161 gctggaattt gggtgaagag tactggggct acgcgaaaaa taaactctgg tcgccgtttg
     2221 gctatccggt tcaccatgcc aatgaagcgt cagcgcaggt cggttccatt gttaactgta
     2281 tgttcaaccg tgactgcatg acgcataccc atatcaattt tattggttcg ggtttgccat
     2341 tgaaactgca gcgtgaagtg gcgaaagagc tttttggttc tgaagatgct tacgatgaaa
     2401 ccaaaaacta cacgccaatc aatgacgcaa aaattaaata tgcaaagtgg tcgctgttgc
     2461 gggtctgttt gcataacgcc gtcaccctgt gcaactgggt ctggccaatg accgtttcgc
     2521 cgctgaaaag ccgtaattac cggggcgatc tggcgcttga agccaaattc ttcaaagcga
     2581 tcaccggcga agagatgact caggaaaaat tagatttagc tgcagagcgt atctttacgt
     2641 tgcatcgtgc ctacacggta aaactgatgc aaaccaaaga tatgcgtaat gaacacgatc
     2701 ttatctgttc ctgggtattc gacaaggatc cgcagatccc ggtctttact gaaggtactg
     2761 acaaaatgga tcgtgacgat atgcatgctt cgctcacgat gttctacaaa gaaatgggct
     2821 gggacccaca gcttggttgc ccaacccgcg aaacattgca gcgtctgggg ctggaagata
     2881 ttgccgccga tctggcagca cacaatctac tgcctgcgta aggaatgggt gagatgaacc
     2941 atcgggatga attgccgtta gcgaaagtca gcgaagttga cgaggcgaag cggcaatggt
     3001 tacaggggat gcgtcacccc gttgatacgg taaccgaacc agaaccggct gaaatcctgg
     3061 cagagttcat tcgccagcat tctgcggctg ggcagctggt agcgcgcgcc gtgtttctct
     3121 cgccacccta ttcggttgca gaagaggaac tgtcagtttt gctggaaagc attaagcaga
     3181 acggagacta cgcggatatc gcttgtatga cgggaagtca ggatgattac tattactcaa
     3241 cgcaggccat gagtgaaaac tacgcggcga tgtctttgca agttgtggag caggacattt
     3301 gtcgcgccat tgcacacgcc gttcgttttg agtgccagac gtatcctcgt ccttacaaag
     3361 tcgccatgct gatgcaagcg ccatattact tccaggaagc gcagattgaa gccgcgatcg
     3421 ccgcgatgga tgttgcacct gaatatgccg atatacggca ggtggagtca tcgacggcgg
     3481 tgttgtactt gtttagcgag agatttatga cctatggcaa ggcttatggc ttatgcgaat
     3541 ggttcgaggt agagcagttt cagaacccct gaaatttcag ggagatgtca tggatagggt
     3601 ggacagttgc agccactgca ttaggggata atcagatgtc cttcactcga cgcaaatttg
     3661 ttctggggat gggaacagta atctttttta ctggttctgc gtcttctctg ttagcgaaca
     3721 cgaggcaaga aaaggaagtt cggtatgcca tgattcatga cgagtcacgg tgcaatggct
     3781 gtaatatttg cgcccgcgca tgccgtaaaa ctaaccacgt ccccgctcag ggaagccgtt
     3841 tatcgatagc acacattcca gtcaccgata acgacaacga aacgcagtat cacttttttc
     3901 gccagtcatg tcagcactgc gaagatgctc cgtgcatcga cgtatgcccg acaggggcgt
     3961 cgtggcgtga tgagcagggg atcgtgcggg tagaaaaatc gcagtgcatc ggctgcagtt
     4021 attgcattgg cgcttgtccg taccaggttc gctaccttaa ccctgtcact aaagtggcag
     4081 ataaatgtga tttctgcgct gagtcccgac tggcaaaagg ctttccgccc atttgcgtca
     4141 gcgcctgccc ggaacacgca ttgatctttg gtcgcgaaga tagcccagaa attcaggcgt
     4201 ggttgcagga taataaatac tatcaatatc agctacctgg tgccgggaaa ccgcatctgt
     4261 atcgccggtt cggtcaacat ttgattaaaa aggaaaatgt atgaacccgt cgcaacatgc
     4321 tgaacagttt cagagccagt tagcgaacta tgtgccacag ttcactcccg aattttggcc
     4381 ggtgtggttg atcattgccg gagtattgct ggttgggatg tggttggtgc tggggctgca
     4441 cgccttgctt cgtgctcgtg gcgtgaagaa atcagccacc gatcatggtg agaagattta
     4501 tctttacagc aaagcggtca gattatggca ctggtcgaat gcgttactct ttgtattgtt
     4561 gctggcaagt gggctgataa atcactttgc gctggtgggc gtaactgcgg ttaaaagtct
     4621 ggttgcggtg catgaagttt gcggattttt gttactggca tgctggctcg gctttgtgct
     4681 gatcaatgcc gttggggata atggtcacca ctatcgcatt cgtcgtcagg gggggctgga
     4741 acgagcggca aaacaaacgc gattttattt gtttggcatt atgcaggggg aagaacatcc
     4801 tttcccggca acaacccagt ctaaatttaa tcccttacag caggtcgcct atgttggtgt
     4861 catgtatgga ttgctgccgt tgttactatt gacggggctg ttgtgtctct atccgcaagc
     4921 cgtgggagat gtgtttcctg gcgtaagata ctggttattg cagacacatt ttgctctggc
     4981 atttataagc ctctttttta tcttcggtca tctttatctt tgcaccacgg ggcgtacgcc
     5041 acacgaaacc tttaaaagca tggtcgatgg ctatcaccgg cactaacgaa tgagcattac
     5101 acaagcagac ctgcgagaat ggcgtatcgg agcggtgatg tatcgctggt tcctgcgcca
     5161 tttcccacgc ggcggtagtt atgccgatat tcatcatgca ctgattgagg aaggatatac
     5221 ggactgggcg gagagtctgg ttgagtatgc gtggaaaaaa tggctggcgg atgaaaactt
     5281 cgcgcatcag gaagtttcct ctatgcaaaa actggccaca gaccccggag agagaccatt
     5341 ttgcagtcag tttgcccgca gcgacgatca tgctcgcatc ggctgttgtg aggataacgc
     5401 cagaatcgca accgctgggt atgccgcgca aattgccagt atgggttata gcgtgcggat
     5461 tggaagtgtc ggctttaaca gtcacattgg cagctccggt gaacgcgcac gggttgctgt
     5521 aaccggtaat tcttcgcgga taagcagcgc tggagatagt agtcggattg cgaataccgg
     5581 aatgcgagta cgcgtttgta cattgggtga acggtgtcat gtcgccagta atggtgacct
     5641 ggtgcaaatc gccagctttg gcgcaaacgc cagaattgcc aacagcggcg acaatgtaca
     5701 catcattgcc agcggtgaaa attcaacggt agtgagcacg ggcgtggtcg actcaattat
     5761 tctcgggcca gggggcagcg ccgcactggc atatcatgac ggcgaaagag ttcgcttcgc
     5821 cgtcgccatt gaaggtgaaa acaatattcg agccggagtc cgctatcgtc ttaatgaaca
     5881 gcatcatttt gttgagtgct gatagcttta cctttaatca aacgaaagac gccgacgagc
     5941 acgggatgca ggttttacga ccgcccgagc catcgcctca ccaatttctg cacatgccgt
     6001 aagtccctga acgaaaggct ggtcgtgcat taaccagggc aacgcgccga acgctacgcg
     6061 cccacgaata tcttcgggtt gctgtaacgt ataatcttca ccaacatcag ggatttcatc
     6121 ccctgttttc tgcaattgtt cgcgtagtcc agggaaagga atatctttca ctttaagcgg
     6181 acgctgcccg cgggcatcaa taaaaacgtc aaacgagtag ctgttgtctt ccgtattcag
     6241 gacggtgcgc gactcgttaa tttccatttt gtagtcttca ccgagagcga gaatatgaat
     6301 gattcccgct tcacgtaagg ccagcagccg acgaatagac tctgaaggga ttgcggcata
     6361 attatcgatg aaaacccggg caaggccttt actgaaccgt ttatggtcgt gttcattcag
     6421 atgtggaaca atttcctgta ctgcttcatg caggcgcaga atggcataac gccagggaac
     6481 agtatgtttt tctcgtttat tgcgttcgac ttcctggaga tttttttctg cccagtcaaa
     6541 tgggtcgcgt tgtttgcgct cggcaaacca ggcttgagca aaggaatcga cattcaggct
     6601 ctctaaggct atgcgttggc tccagtctgg atcagcaaac ttgatttcct ctactatcaa
     6661 tctaaatacc cgatccaaaa ggccatattc gcctttttga atctcagcat ttaatgcctg
     6721 atcagtgacg atgtgtaagg gctcgtaggg aatagggcaa tagaaatcgg cttcgggtaa
     6781 aatacccgtg cgcgacatta acgtaatatt tagcttttca cttgcgttat cgcggtgaaa
     6841 aacgacgtgt tgtttatcat cttcaatgaa cgaaccatgc tgaattgcca ctgccattgc
     6901 cgcatccagt ccgctcaagg atgttcccat aatacccacg ttacacgcat cgacctttgc
     6961 ttccatcagg cctgaccacg ggctgggaaa atacgttcgg gttgcttgtt cttcatcagg
     7021 ccagacgtga cccgtggcaa tcaccgctaa atcaaacgtc tcgctgggta aatcctgatt
     7081 tgtagcgagc atgacgccag catttgtaat ttgcagatcg gtaacctggc atgattcata
     7141 aacagccact gcaaattttt gctttcgtgc ctggtctact aatcgtaaaa attgatcgcg
     7201 gaaatattcg cccagcagaa ttcgcggtaa aaactgacga tcgtgcaagg tttctttttt
     7261 aacgccataa cgctggagat gactggcttc ttgcttttgt agccattcga gatacgtaca
     7321 ataaatcggc ggtatttcaa tactggcaat atttgccagc atcatttttg agttttcctc
     7381 atcactgtat ggcatcccga caccggcctc gtcagcctgc tcgaagatag aaatagaaag
     7441 tggagtttgt tgctgtagaa gcgagaataa ggtgtagatc cccgtaggcc cggcacccac
     7501 aattgcaatt tttttcattg ttacttacct gtacgcattc tttcgaattt agtgttatca
     7561 gataagtttg gttgagattt ttagaatttg agaaatttca gacagggatg aaaagggcgc
     7621 taaccgccct tgattaaatt ctgtcaggcg agttttgcct gattgatttg actcagtggc
     7681 tcattgacat cgaaaacttt ggcgacaact tcctcaacgc cgagattttt cagtcgggca
     7741 gtgtgttttt ccagataggc aagggcgctt ttttcatcag tgaaaaggta gatcccaccg
     7801 gcttcgtggt tcttttcact ttcggtccat accttccaca gaaaaccagg ttcctgatta
     7861 atcgactcag caagcggttt aagctgctca gccattgcgt cgccaaatgg gccattgaaa
     7921 gaaaaatgaa gttgtaacaa agttgccata gcgattctcc ttctgataac gcatacgaag
     7981 acagtgtaaa tcttttaacg tattggatac agagaagaaa cagagggaaa aaggagaaga
     8041 ccagattaag aaaatctggt gcgtccgagt ggactcgaac caccgacccc caccatgtca
     8101 aggtggtgct ctaaccaact gagctacgga cgcaggatgg tgcgttcaat tggactcgaa
     8161 ccaacgaccc ccaccatgtc aaggtggtgc tctaaccaac tgagctatga acgcaacgtt
     8221 gtaggtgaca acggggacga atattagcgg cagagtggga aggtggcaag aggcaaaacg
     8281 taattttctg cgctatttcg accgtttgca gagcttttaa gcaaattggc tatattttgt
     8341 tgatttgcaa gggcgatttt tattcaggat cgcatttaca tctgatacaa cccaataatt
     8401 cgtgtatcag agtttaaaca gagttgtttg ccaccatctc aaatctgtcg tggatcaatc
     8461 ctccgtagcc tttacactta ggtagcaata aaggcacaca gatgcagcaa aggaataaat
     8521 catgggatct gatgcgaaaa acttgatgag cgacgggaat gtgcaaattg ttaagaccgg
     8581 cgaggtcatt ggcgcgatgc aacttactga aggcgagttg attgttgaag ctggcggaag
     8641 agccgaaaat accgtggtca ctggggctgg ctggttgaaa gtgacaaccg gtgggatcgc
     8701 caaatgcaca cagtacggta acaatggcac gctatcggtc agcgacggtg ccattgccac
     8761 agatattgtt cagtccgagg gaggcgcaat tagtctctct acgctcgcta cggttaatgg
     8821 ccgccatccc gaaggtgaat tcagcgttga taaaggttat gcctgcggtt tgttgctgga
     8881 aaatggcggt aacctgcgtg tactggaagg ccatcgcgcg gaaaaaatta ttctcgatca
     8941 agagggcggc ctgttggtca atgggacaac ctcagcggtc gtggtagatg aaggtggtga
     9001 attgttggtg tatccaagtg gggaagccag caattgtgag attaatcagg gcggcgtttt
     9061 tatgctggcc gggaaagcca atgatacgtt gcttgctggt ggcaccatga ataatctcgg
     9121 tggtgaagac tctgacacta ttgttgagaa tggagccatc tatcgtctgg ggacggatgg
     9181 acttcagctc tacagttccg gtaagacgca aaacctgtcc gttaatgtgg gtggtcgggc
     9241 tgaagtgcat gccggtacgc tggaaaatgc ggtaatacaa ggtggaacag tgatcctgtt
     9301 gtcacccacc agcgcggacg aaaattttgt cgtagaggaa gatcgcgcac cggttgaact
     9361 gaccgggagt gttgcattac tggacggcgc ttcaatgatt attggctatg gcgcagatct
     9421 gcaacaatca acgattactg tacagcaggg cggtgtattg attctcgacg gcagtacggt
     9481 aaaaggtgac agtgtcactt tcagtattgg taacatcaat ctgaatggcg gaaaactgtg
     9541 gctgatcact ggtgcggcaa cgcatgtgca gctgaaagtg aaacgcctgc gcggagaggg
     9601 agcgatttgc ctgcaaacca gtgcgaaaga aatttcacct gacttcatca atgtgaaagg
     9661 ggaagttacc ggggatatac acgttgagat aacagatgcc agtcggcaaa ctctgtgtaa
     9721 cgcactgaaa ctacagccag acgaagacgg gattggcgca acgctccagc ctgcgtaaaa
     9781 acacaggcgc aacccgtgaa ggttgcgcct tgtctttatt agcgggatgc tcgttgcaga
     9841 atgatggctg acggcagacg ttgcaggaac cgcatacgca gcatcatcat aatggctgcc
     9901 gacgtcaggc caataataaa gcctatccag aagcctgctg gccccatagg ttcaacgacc
     9961 agatcggtca gtgccagaat atagccgctt ggcaagccca gcacccagta agccgtaaag
    10021 gtaatataga aaatggaacg cgtatcttta taaccacgca aaatcccact gccaatcacc
    10081 tggattgagt cagaaatctg atataccgcc gccagcaaca tcaaatgcgc cgccagcgtt
    10141 acaacctcgg gattgtcgtt gtacaacagg gcgatttgct cccgcagtga aaccgtgaat
    10201 atggccgtca gggttgccat acagacaccc accataagcc cggtccgcgc agcggtttgc
    10261 gcatccagcg ttgagccctg acccagacga taacctacgc ggatagttac cgctgccgcc
    10321 agcgacattg gaagcacgaa cattagtgaa ctaaagttca gggcaatctg gtgtcctgcg
    10381 acatcaacaa taccgagcgg agacactaac agagccacga cggcaaacag tgtcacttca
    10441 aagaatagtg ccagcgcaat cggcaaaccg agttgaatca gtcgtttcat aaccgcagga
    10501 tcgggtttcg cggtgccttt ttcgttacga atatcgcgca tggagcgggc gcgtttaatg
    10561 taagaaacca tggcaaggaa catgacccaa tacaccgccg cagtagccac gccacaacca
    10621 acgccaccga gctcaggcat accgaaatga ccataaataa agatatagtt caccgggatg
    10681 ttcaccagca ggccgataaa gcccattacc atacccggct tggtttttgc cagaccttca
    10741 cattggttac gggcaacctg gaagaaaaga tagcctggcg cgccccacag caacgcacgc
    10801 agataaccca cggctttgtc cgccagagcc ggatcgatgt tttccatgga gcggataatg
    10861 taacctgcat tccacagcac cagcataatg agaacggaaa caaaacctgc cagccagaaa
    10921 ccttgtcgca cctgatgcgc aatgcgctca cgtcgaccgg aaccatttaa ttgcgcgata
    10981 accggcgtta atgccagcag cagtccgtga ccaaagagga tcgccggaag ccagatagaa
    11041 gtaccgatag cgaccgccgc catgtcggtg gcactatagc cgcccgccat cacggtatcg
    11101 acaaaaccca tcgcagtttg ggcgatttgc gcgagaatca ccgggattgc taatgctaat
    11161 aacagacgcg cttcactgat atacttctgc acgtgaacac cttttatttg tagttatatg
    11221 agagactaaa aagccgcctg aatgggcagc aaaaagaaga agcaggggaa attcagtcta
    11281 ttgtagcgag gatttattaa ttctccagtg aaaaaatagc cattcttacc ccttcactgg
    11341 taagggcgtt tttcacctgc tattgtgctg aacagaatgt cttaactgat ttcaggagtt
    11401 gtaagtatgt ttacggggat tgtacagggc accgcaaaac tggtgtcgat tgacgagaaa
    11461 ccaaattttc gtacgcatgt ggtggagtta cccgaccata tgctggacgg cctggaaacc
    11521 ggtgcgtccg tggcgcataa cggttgctgc ctgaccgtga cggaaattaa cggcaaccat
    11581 gtcagttttg acctgatgaa agaaacgtta cgcattacca atcttggcga tttaaaagtg
    11641 ggggattggg taaacgttga gcgtgcggcg aaattcagtg atgaaattgg cggacactta
    11701 atgtcaggtc atattatgac caccgctgaa gtggcgaaaa tattaacctc ggaaaataat
    11761 cgccagatct ggtttaaagt ccaggatagt cagttgatga aatatatcct gtacaaagga
    11821 tttattggca tcgacggtat tagcctgacc gtcggcgaag tcacgccaac gcgtttttgc
    11881 gtccatttaa ttccggaaac actggaacgc acgactcttg ggaagaaaaa acttggcgca
    11941 cgcgtcaaca ttgaaatcga tccacaaact caggcagtgg tagatacggt agaacgtgtg
    12001 ctggcggcac gagaaaatgc catgaatcaa ccaggtacag aagcctgatc tgaaatcgcc
    12061 cccggagagg gggcgataga atagccttta gcgagcgact cgaaggccgt tttcaacacc
    12121 gcgtgagaac accacctgcc agagctgaat atcacgggcg cggaaagcac ctgcgcaggc
    12181 attcagataa taggtaaaca ttcgtttaaa gcgttcactg tagttatccg caatttctgg
    12241 ccatgcggcg aggaatcgtt cataccacgc catcaacgta gtatcgtaat cagcaccgaa
    12301 gttatgccag tcttccatca caaagtgggg ttcgctggac tgagcaatct ggcgtacaga
    12361 gggcaggcaa ccgttcggaa aaatatattt attaatccag ggatcaacat tcagatcggt
    12421 ttttttcgaa ccgatagtat ggagcaggaa tatgccttcc ggtttcaaat tacgatccac
    12481 caccgcaaaa taggtatcgt aatttttcgg tccgacgtgc tcgaacatcc ccacagaaac
    12541 aatacgatca aactggtcgt tcaggtcacg ataatcttgc agcaaaatgg tgacatccag
    12601 gccttcacag cgttcctgag ccattttttg ctgttcggca gaaatggtga cgcccaccac
    12661 gcttacgtca taattagatg ccatgtagtg tgccagtccg ccccagccgc agccaatatc
    12721 cagtacgcgc atcccaggtt ttaactgcaa tttttcacaa atcattttga gcttcgcctg
    12781 ctgggcagat tccagattat cggcatcttt ccagtaagcg caggaatatt gcatgaaggg
    12841 atcaagcatg cggctgaaca agtcattacc caaatcgtaa tgctctttgc cgactatcca
    12901 ggcacgtttt ttactctgca gattgaagag acgggcgccg gcaatacgca gcgtgtcttt
    12961 gaaatgatgg gggagttggt tctcgagacc tgcgcgtaag actttgctaa aaaacatatc
    13021 cagtcggtca cattcccacc agccatccat ataactttcg cctaacccca aagagccttc
    13081 ttgcagaacg cgtttaaaaa aatcggggtt tttcacacga atatccgccg gggcagaacc
    13141 gttaatggct ataccggcac ggctaagtaa ttcgttggcg atacggtacc agttgtcatc
    13201 cggtacactg acttcttcta tacacgatga actcatagtt tctccatcac tggtcggtga
    13261 tcagaacctt aaaacagcgt agacgctttt tgggctttgt gagaaatctc gcagaaaaaa
    13321 ccgcgagtct ataatccgac gtagaacaga gggaaaggag gaacccttgc cgaaatgacc
    13381 atccatagtg agtcgggaac gatcctgtcc ccgtaaaaac ataatattta tcgtagcttt
    13441 aacaactcga aattagtata ggccgctaat ttcaatcata caacaaaatt ttgttagcag
    13501 cctaagtata agtatatcga tatagatcag tgtgattcgc tatgagcgac ttcggcattg
    13561 ccatgattct ggcagccaac ttcttcacaa cgttgcatca tgtaacccag cgcgaccagc
    13621 actactgttg ataacatcac gctggtggtg gtgagcaatg gcgtgctgat actgatcagc
    13681 caggaaacta ccagacttgc gaggaagcac agacccagtt gaagagtgtt ctgcaacgct
    13741 gcggcgcgac cagttgcgtg tgggaaggga cgcagcgcct gggcgacaac aatagggtag
    13801 atcgcgccat tggcaatcgc catcacacag aatgggatca ggatttcgac cagcgacaca
    13861 tggctaatga agcccgcagc ccaggtcgca atgacgctga cagcaaacag caccagcaac
    13921 cacggtaata actgcttgcc ttgccatttc tgcagcgcgg cgcgacagcc ataaccacca
    13981 atcagaaacg cgatagtttg cgggacataa cttaaaccaa taactgccgg gctgtagccc
    14041 atttcactaa ggatgaacgg tgaaccggtc agccatgcaa aaaaactggc tgaacaggcc
    14101 gcgtatatca gcacgttgcc gcgataggtt ttagaacgta gcaggtcggt aaaggtcaga
    14161 ccatcctgac tattgttacg ggccttcgtc gtgggtttga gccagaaaat aggcagaatc
    14221 agcaccacgg taatggcaaa cagggtggcg aaaatcgcct gccaggaaaa atggaccagc
    14281 agccagcttc ctaacagagg agccagggcc ggagatagac ccaccagcgg catgatggtc
    14341 gcaaaaattc ggttaacttt ctgtgaagga taataatccg tcactaacgc ttgccagata
    14401 accgccgcgg cgcagacacc cacagcctgt acaaaacgca ataccagcag cgtagcggcg
    14461 ttttctaccc acagcatccc cagactacct aacgcaaaaa ttgtcaggcc gattaataat
    14521 accggtttac gaccataacg gtcggagagc ggcccccaca gaagctgggc tgcggcaaaa
    14581 ccggcaagga acagactaag gctggcactg acagcagacg caggcgtttg caggtcggcc
    14641 tgtatggcgg cgaaagcagg cagatacata tcggttgcca gaaaaccgag tacgctcaaa
    14701 cccgccagcc agactaaaaa tcttttccca ggttgcatgt atattctcta aatctctcaa
    14761 ttaaacaccg ccgcaaagtg tagggagtga aattagtgtt gtgaaacggt aatatttgat
    14821 gactggtttc aaaaattttg caggcagact atgtggtcag aatattcact cgaagttgtt
    14881 gatgcggtag cgcgtaatgg tagttttagc gctgcggcac aggagctgca tcgcgttcct
    14941 tctgcggtca gctataccgt gcgtcagctg gaagagtggc tggcggtgcc gctgtttgaa
    15001 cgacgtcacc gtgatgtgga actgaccgct gccggggcgt ggtttctcaa agaagggcgc
    15061 tctgttgtca aaaaaatgca gatcacccgc cagcaatgtc agcagatagc gaacggatgg
    15121 cgtggtcagt tagctatcgc agtggataat attgtcaggc cagaacgtac acggcagatg
    15181 atcgttgatt tttatcgcca ttttgatgat gtcgaacttc ttgtctttca ggaagtgttc
    15241 aacggtgtct gggatgcgct ttccgacggg cgcgtggaac tggcgattgg cgcaacacgc
    15301 gcgattccgg taggcggtcg ttatgccttc cgggatatgg ggatgctaag ctggagttgc
    15361 gttgttgcca gtcaccaccc gctggcgttg atggatggcc cgttcagcga tgatacgttg
    15421 cgcaactggc cgtcgttggt gcgcgaagac acctcgcgaa cgctacctaa acgtattacc
    15481 tggttgctgg ataaccaaaa aagacttgtc gtacctgact gggaatcatc ggcaacctgt
    15541 atctcggcag gattatgcat agggatggtg ccaacacatt tcgccaaacc gtggcttaat
    15601 gaggggaagt gggtggcatt agaactggaa aacccctttc cggattcggc atgttgtctg
    15661 acatggcagc aaaatgatat gtcgcctgcg ttaacctggc tgcttgagta tctcggcgat
    15721 agtgagacgc tgaataagga gtggctgcgg gagccggaag agactcccgc aacgggtgat
    15781 taacgacgat agtcgcggaa cgggccgtca gccacggagc ggcgttcaat caagcgcgga
    15841 tgcacttcaa tggactgcgg ttcttcacgt ttgttgacga tacgatccaa cagcatgttg
    15901 aacgctgttt cacccagcga atcttttggc tggtggatcg tggttagcgc cggcgtaaaa
    15961 tagcgggcgt tgcgcacgtt atcataaccg atcagcgaaa catcctgcgg gacgcgcaga
    16021 cccatttcat cagcagcaca aagtgcgccc attgccatga tatcgccacc acagaagacg
    16081 gcagtagggc gatgcggctg cgacagtatt tgctgcatgg cgcgataacc agattcgggt
    16141 tcaaagtcac cctgaacaat ccagctttcc ggcaccttga tcatcgcttc ttccatcgcc
    16201 ttcataaaac cggcaaggcg gcctgcgccg gtgttacgtt ccagcgggcc ggggatgacg
    16261 ccgatttcgc ggtgaccgcg ttcaatcaga taacgcccgg ccatgtagcc gccttcgaac
    16321 gcgttatcaa tgaccgcatc ggtgaagtca gcttttgctt caccccagtc catcaccacc
    16381 attgggatat ggcgatactc ttccagcatc gccagcaacg gctctgggta ctcagaacac
    16441 atcaccagca gaccatcgac gcgtttttgc gccatcatcg acagataagc ccgctgtttc
    16501 tcaagattgt tccacgcatt acccagaatc agggtgtaac ctttctggaa gcaatttttt
    16561 tcaactgctt caatgatctc ggcaaaatag gccgcttcgc tgctggtcgc cagcaaaccg
    16621 atagacttgg tgtggttaac cttcaggcta cgcgccaccg cgctagggga gtagtgtaat
    16681 tctttaatcg ctgcccacac ggcgttgcgc gtttcttcag cgacgaaacg tgttttgttg
    16741 atcacgtgtg acacagttgt agtggaaacg tttgctcgtt tcgctacatc ttttattgtt
    16801 gccattccat ttcactccag accctaactt cagctcctgc aaaatcgcaa ggtaaacgtt
    16861 tgcctttaca caccttacgc acaacaaaag tgttgcggta cgccggaaaa acgacacgga
    16921 gcgtcaggaa ggggtcaatg gccagtacgc aaatagtgta agcgtggaat tttggccgat
    16981 cttgacgaaa aggggaagtg gtaaaaacac ttatcattat aaatgacggg agattttttc
    17041 atcacagtgt gtaaaaatgc gtaatatcac tttttgtgtg gtaataaaaa aggagaaaac
    17101 ttgatgagca ccgaccttaa attttcactg gtaacaacga ttatcgtcct cggtttgatc
    17161 gtagccgtgg gtttgactgc cgcgctgcac tgatttcctc tcgagggagc gcgttctccc
    17221 tcgcatctcc ttcctgtttg ttagcaaaat ggcaaaagtg ttttctcttt ttgttatttt
    17281 catttttttg tgattgctgt catgctttcg ccactttaat gaatatcgcg ttatgacgcg
    17341 aagaggtgga gataaggcat gaaaattaac tatccgttgc tggcgctggc gattggcgcg
    17401 tttggtatcg ggacaacgga gttctcgcca atgggcttgt tgcccgtcat tgcgcgcggt
    17461 gtggatgtct cgattcccgc tgccggaatg ttaatcagtg cctatgcagt tggcgtaatg
    17521 gttggcgcgc cgctgatgac gcttctactt tctcatcgtg cccgccgcag tgcgttgatt
    17581 ttcctgatgg caattttcac gctcggcaac gtactttccg ccatcgcgcc ggattatatg
    17641 accctgatgc tttcacgcat tttgaccagc ctgaatcacg gagcattttt tggtttgggt
    17701 tcagtcgtgg ccgcaagcgt ggtgccaaaa cataaacagg ccagcgcagt tgccactatg
    17761 tttatggggt taaccctggc aaatatcggt ggcgtgccgg cggcgacctg gttgggtgaa
    17821 accatcggct ggcggatgtc atttctggca acggcggggc tgggagtgat ttcaatggta
    17881 agtctgttct tctcattacc taaaggtggt gcaggggcac gacctgaagt gaaaaaagag
    17941 ctggcggtat taatgcgtcc gcaggtactg tctgcattgc tgacgacggt actaggagct
    18001 ggtgcaatgt ttactctcta cacctatatc tctccggtac tgcaaagtat tacccacgca
    18061 acaccggtgt tcgtcacggc aatgctggtg ctgattggtg tcggattctc tatcggtaac
    18121 tatctcggcg gcaaactggc agatcgttca gttaacggca cgttgaaagg ctttttgttg
    18181 ctgctgatgg tgattatgct ggcaatcccg ttcctggccc gcaatgagtt cggcgcagct
    18241 attagcatgg tggtgtgggg cgcagcaacc tttgcggtcg taccgccgtt acagatgcgc
    18301 gtgatgcgtg tcgccagtga agcgccaggt ctgtcttcat cagtcaatat tggtgccttt
    18361 aatcttggaa atgcgctggg agcagctgct ggtggtgcgg taatttccgc tgggctggga
    18421 tacagctttg tgccggtgat gggggcgatt gtcgcgggac tggcattatt gctggtgttt
    18481 atgtcagcca gaaaacaacc tgaaacagtt tgcgttgcta acagctaatt aaacaaaaac
    18541 gcagaaggat aaccttctgc gttacagttg ctgacaacgt gcgatttatc ttgccgaatg
    18601 cgctgttaac gcctcatccg cgttggcatg gtaagcgaag cgcatcaggc aatactgcat
    18661 ttgccatcag ttattatgca gcgagatttt tcgctacgaa ttcccagttc accagcgccc
    18721 agaagtgctc aagatagcca ggacgtgcat tgcgatagtc gatgtaatat gcgtgttccc
    18781 agacatcaac ggttagcagc ggagtcgcat cggtggtcag cggagtaccc gcgttagagg
    18841 ttgaaacgat agccagtttg ccatcgctgt ttttcaccag ccaggtccag ccagaaccaa
    18901 agtttttgat cgctgcatca gtaaactgag ctttgaaatc ggcaaagctg ccaaaagatg
    18961 cggcgatagc ttcagcgact tttccagtcg gttcgccacc ggcgttcggt gccaggcagt
    19021 tccagtagaa agtatggttc cagacctgag ctgcgttgtt gaatacgcca ccttcagagc
    19081 tgcgaataat ctcttccagt gatttacctt caaacgcggt acctttaatc aggttgttca
    19141 ggttagtgac ataagtctga tgatgcttgc cgtagtgata ctcgatagtt tccgcagaaa
    19201 tgtgcggtgc cagagcatct ttagcatatg gtagtgcagg taattcgaat gacattgcta
    19261 ctctccttta ttattaattt gcatacgtac aatagcctta ttgtgcgtat ggatagggta
    19321 gcaaattaaa aggtgagaca aaagatgaac ttaccctgtt gccgtaacaa cagggtaaag
    19381 ttttagcgaa gtgtttttgg ggtcattacc cgacgagcgc caacatagtg gcgctgccag
    19441 tagtcttcac tgagagaagt gatttgaatt tcctgacctg tgcgcggtga ctgaataaat
    19501 ttgccgttgc cgacatacac gccgacatga tcggctgtac cgcgtccctg agtacggaaa
    19561 aagaccaggt cgccgttttt cagttcacta cgttcgattg gcgctgcatc acgcaggtga
    19621 tacatttcat tcgccgtacg cggaatacga attttcacca aatctttata agcgtaataa
    19681 accaggccgc tgcaatcaaa accggtacgc ggtgagctgc caccccaacg atatggctta
    19741 ccaatttgct gcatcagttt attcattgcc acttttgtgg ctttttgcac cttcgctttg
    19801 tgtgcgtcgg caagggttcc tgacgcggca tttttgactt tcacacaatg cgatttatag
    19861 cccttacgtt tggtacactt ttcggtaaag ctggctgagg ccgttttatt gacggtatgt
    19921 ttgctgcgag ttttggcgtt tttagaagcg gtaatggaag attttttaac ggaagaagcg
    19981 gttttgcttt tggtcgtact tttactggcg gcttttttcg ctgttttctg gctggctttc
    20041 tgggtctttt tggtggttgc cgtgcttttc tttttatctg cttttttggt gatatgagtg
    20101 gtagcagaac tctccctggc ttgctttgaa gcatgggcca taggcgttaa aggcagggtg
    20161 gtaaaaagta aagcacagag cgtgatcgaa atacggttta tccgcgccac tgagcgtccc
    20221 tctgttaagg caggtctatc atgcctgcgt ctgatttaca aaacttgatg attctaatgc
    20281 atagaagaag agaaagttaa tagcttttat tgttaaaaaa tacgtttcgt tggcgagcgc
    20341 aaaaaaatgc agcagtgaaa gctaaaaatg gttataaata cgccagtttg ccgtgcatct
    20401 gggaagaaac gaatatttga aatgcaacaa atagagcaaa gggataaaat agctaaagcg
    20461 tgatgaaaat gttattttcc gatgccgcac tgacccgcta caatgtcaga ttactgccgt
    20521 aaagaagttt gaggaagcaa gacaatgagc accactatcg aaaaaatcca acgccagatt
    20581 gctgaaaacc cgatcctgct gtacatgaaa ggttcaccga aactgccgag ctgcggtttc
    20641 tctgcccagg cagtccaggc gcttgccgca tgtggcgaac gttttgccta tgttgatatt
    20701 ctgcagaatc cggacattcg tgcggaactg ccgaaatatg ctaactggcc gaccttcccg
    20761 caactgtggg ttgacggcga gctggtcggc ggttgtgata tcgtgatcga aatgtatcag
    20821 cgtggcgaac tgcagcagct gatcaaagaa actgccgcta aatacaagtc tgaagagccg
    20881 gacgcggaat aatccgtcct gcaatactga cggatgcgaa cgggcatccg tcagtccttt
    20941 ctctatcccc aatccagccc ttgtggcgaa cttgaaaatc ccgcttcacg cagcagcgta
    21001 aacatcggcg tttgccggac cggtagatca ttcacttctg ttagcgtaaa gcgcagccgt
    21061 ggttcgcgac gcagtgcggt agtcagcgcg tggaaaacct ctggggcgag taattcctct
    21121 ttttcctgcc agaccagcat ttttttgcca ccttgcgcca gatagagtaa caatttgccg
    21181 ccagaaacga ccaccagcgc acccgcccga cgcgttggaa ccagcgttgc cggatgtgca
    21241 ggccagggca gaagatttcc ccacacatta gccggatcgt tggcggagag cgccactggt
    21301 gtatagtggc gcgtttgcgc ggcttgtgtc gccaggtcgc gcaagcggtc aatagtcaga
    21361 cgttcagcga attgcgcgcc acccagacct tctacaaaac gacctcgcat aattcgcccg
    21421 gaatcttcca tacttcgaca aagcgtttgc atcgatggaa acccgccagg gatattttcg
    21481 gctatcaccg cctgacgact gatgatgccg tagcggtcga gcatattttc cgccagcgcc
    21541 agcatccttt cggtatcgtt tagtggctcc acctgcaata acgaccagcg tccagccaga
    21601 tttggtgtgt tgtaagatac ccgcggcgag acgggttgtg catagacagg acgcccacga
    21661 cgagcccggt gactgcggcg agttgaggtg cgtgcgttgg aactgctgcg ggtgagggcg
    21721 cgtaacggtg cccaaatatc gctggtgatg acgccttgcc agaccagcgc ccataacgcc
    21781 tcttgcagtg ccgagggatc aaccgattcg ccgattttgt cgcgtatccg ttggctgatt
    21841 tgttgtgcaa accaggctcc tccgtcagcc agaacagcga ctatcgcttg ttgcagcgcc
    21901 gaacgattcg cctgatccgc ttcggcggga gtgaacgatt ctgcggcata ttcctgtaga
    21961 tgcagtgcca ccagaccgtc atcttcaccc agcttttttt gtcccgacca gataaccgct
    22021 ccggttgcca gtaattcatc gagcatttct ggcgaatagt cgcgtacgcg ggcaggcagg
    22081 atctggcttt cccagagtga ggcgggtaaa ccgactccgg caagctgttc gatcacccgc
    22141 atcacgccat ctacgccctc ataaacgcct ggcgatgttg aggcaaagag cgccgggcta
    22201 ccatcggtgg cgggtaatac gccctgacgt tccagcagca atcgcgcata ggtcgtggct
    22261 gcaacgggac gcgtcgcttc tctggcggct tgcagcgagc gcaaacgcag acgacgaaat
    22321 acttcatcgc tcacccagat gtcttgttgc agattcatca ccagaccctg ttcgcgcagt
    22381 tgctgaagct gctcttcgac aatggcaata ccgagactaa actcatgagc cagttgttca
    22441 gccgtgacca gagcatgagc ccggagatag cgtagtaaga ggtcgcgaag cgggtaactg
    22501 actctatgca aataaatctc tggcagtgac tctggtagtc gtacgccgag ggcatcgcgc
    22561 agcctggcgg catcatccat acaggcaaga cgctcctgtc cgctaatcat cgccgggaag
    22621 attcgtttta ccgcaagaag attttccaga tatgaaacaa cctcttcact gctgcctgtg
    22681 tgccgttgcg ccaggtcttc aacggtcatt ggccccagtt cgcgcagcag gtcgaacaat
    22741 ccttcttcac ctttcgctct tctgccagga gccagtcgtt gcaactcttc ttccacctgg
    22801 cgaatgacct gcgggtcgag taattccccc ggatcgacct gcccgagtag attgcgcagt
    22861 aactcgctgt ccagcgacag tacggatgcc cggcgctctg ccagcggggc gtcgctctgg
    22921 tacataaatt ccgcgacata gccgaacaat aaacttgtgg cgaaaggcga gggcgtagta
    22981 gtcgttacat cggatatttg aatttcgcca ccgttcaggc gacgcatcaa acgttccagt
    23041 gcgggaagat cataaacatc ttgcagacat tcgcgtaggg tttcgagaat gaccggaaaa
    23101 tccggatatc cctgagcgat ttccagcaac tgactggcgc gcagccgttg ttgccagagc
    23161 ggggtgcgat ggcccggcgt gcggcccggc attaataacg cccgcgcggc gcattcgcga
    23221 aaacgggcgg cgaaaagtgc tgagctgcct accgcctcgc ggacaatttg cagcaacttt
    23281 tctggttcaa acaaaaaaat cgcggcgtcg ggcaatttac catcagtgtc aggaatgcgc
    23341 gcaacaatgc cgtcatcact ggcgaccaca gacgcatcag cgccccatag cgcatgtatt
    23401 cgcccggcaa tcgccagcgc ccagggttca tgcacccgtc ttccataggg agagtgcaaa
    23461 ataatacgcc agtcgccaat ttcatcacgg caccgttcga ggaccagatg acgactcccc
    23521 ggaacgatgc ccgtcgcgtt gcgctgctcg tcaatcaacc cctgaatatt ggcgatcgta
    23581 ttttcttcag ccagccacgg ggaaatagtc ccggaaaaga acgcgccatc cgccagtaaa
    23641 tggagaaaat cgccgatcat ctcgcctaat tcagccggac gtccgttacc ttcaccacgc
    23701 caaaagggaa gccgggcaga acgacccgga gcaggagtca caatcacctg atcgcgggtg
    23761 atttgctgga tccgccatga ggtagcgccg agagtgataa tgtcgttcac ccgcgactca
    23821 tataccatct cctcatccag ttcacccacc cgccgcgaac cggcctgttc ttcaccttcg
    23881 ggtaataaca cgctatacat gccacgatcc ggaatggtgc caccgctggt aaccgccaac
    23941 agctgagcgc caggtcgggc ggtcaatatc ccggtctccc ggttccagac cagttttggt
    24001 cgaaaagcag aaaaatcgcc agagggatag cgcccggaaa gcatatccag cgtggcgtca
    24061 aaaacacgtc ttggcagatc tttccacggt gcggcacggc gtgcgcggga gtaccattcg
    24121 tctacctgta atgcatccat cgccgcggcg gcaacggtgt gctgcgcaag gacgtcgagt
    24181 ggattatgcg gaggtgtcag gttttccagc ctgcctgcga acatacactc tacaatgact
    24241 gcggaatcga ctaagtcacg ccgggttcgg gggaaaaaca gccctttaga tacaccgcca
    24301 acctgatgtc cggcgcgacc aatgcgttgt aacccactgg caacagaaag cggcgttgcc
    24361 acctgaatca ccagatccac tgcgcccata tcaatcccca gttcgagact ggaagttgcg
    24421 accacgcagc gtaactcacc caatttcagc gcctgctcgg tgattgctcg ttgttcttta
    24481 gagacggagc cgtggtgtga gcgggcaata aaaacgtcgc tactttgtac acggttagag
    24541 gttgcgccag aggtcgactc gaaatgggcc gcatcagcgg cgatagacgg ggaacgctgt
    24601 aagcgtgcgg cgtaaagctc atttaatcgt gctgtcagtt tttccgccag cccccgcgaa
    24661 ttagtaaaga caatggtcga gcgatggcgc aacacttcat caaggatacc cgtttcaata
    24721 tatggccaga tggagccttc ccggccggca tggctgtctt cgccagtgcc gctggcgacc
    24781 gatgagacat catccatatt ggcgactggt acgacaatcc gtatctgcgg atggcgcatt
    24841 gcgggcgggt tgactaccgt aaccgggcga tcgccgccaa gaaatgctgc cacatcgctg
    24901 gctgagcgca cagtggcaga aaggccaatt cgctgtgctg aggtgtggag cagcgcatcg
    24961 agccgctcca gacttaacgc cagatgcgcg ccacgtttac tgcccgctac cgcgtggact
    25021 tcatcaataa ttaccgtttc gacgccgcgt agcgtttcgc gcgcgcggga ggtcagcatc
    25081 agatagagtg attcgggtgt ggtgatcaga atatccggcg gattacgggt gagtttgctg
    25141 cgttcctgtg caggcgtatc gccagtacgg atccccacgc gaagattgac ttccgtttcg
    25201 ccgcgccgcc gccgttcatc ggcaataccc tgtaacggga tctgcaagtt gcgctgaacg
    25261 tcggtgccca gggcttttat cggtgaaata tagaggatgc gtgaggtttt acgtttatgc
    25321 gcctcgcggg tatcttcgcc gccttcgcgg aagagctgat cgagggcgta gagaaatgct
    25381 gccagcgttt tcccggagcc agtcggtgca atcaccagcg catgttcgcc tcgcgccgcc
    25441 acatgccagg tttgcgactg gacagcggtc ggctgtttaa aggcgcgaag aaaccagtcg
    25501 cgggtcgccg gtgaaaacac gtccggcagg ggcgatgaag ggtttggatt atctgccatt
    25561 aacgctattc cccgtgaata tcatgcatga tgcgtggaat agcctcacta tagatgcctg
    25621 aatcattgca tgtcatcagg catcgactcg attacacctc ttcggcggca ggtagcggcc
    25681 agcctcccag acgtttccag cggttgacga tttcacaaaa cagcaccgca gtgcgttcgg
    25741 tgtcgtacag cgcggaatgc gcctgggtgc tgtcgaagtc catgccagcg gtctggcaag
    25801 cctttgacaa tacggtttgt ccgagtgcca gcccggccag tgcagcagtg tcaaaagtgg
    25861 cgaaagggtg gaacgggtta cgtttcagtg aggcgcgttc tgcggcggcc atcataaagc
    25921 tgtgatcgaa attggcgttg tgcgccacca taatggcgcg gttacagccg ctcgctttaa
    25981 tacctttacg tacaacttta aaaatttcgt gcagcgcctc gtattcgctg accgcgccgc
    26041 gatcgggatc gttcgggtca atgccgttga aggcaagggc ttctggttgc aaatttgcgc
    26101 cgacaaatgg ttcgacgtgg aaatgtaatg tggtgtccgg catcagccag ccttgttcat
    26161 ccattttcag ggtgatggcg gcaatctcaa gcagcgcatc ggttttggcg ttaaatccgg
    26221 ctgtttcaac atcgatcaca acaggataaa aaccacgaaa acggtcgcac agaccggtaa
    26281 gttgagcgtt atcggacatc tgattctctt aatacggata aattgcagcg tgcattatga
    26341 caaatattac ttgtcgatgc agtaaagatg cgggcgcgag gagttcacgc ccggcaggag
    26401 attagttgcc cagaccgcga ccggcgtctt tctcttcgat taactcaatt ttgtaaccgt
    26461 ccggatcttc cacaaacgcg ataaccgtag taccgccttt taccggaccc gcttcacggg
    26521 tcacgttacc cccgttttga cggatttttt cgcacgcttc agcggcatta tctacgctaa
    26581 gcgcgatgtg accataagca gtgccgagtt cgtatttatc cacgccccag ttgtaggtca
    26641 gttcaatcac cgcttcttcg gtttccgggc cgtagccaac aaacgccagt gagtatttgt
    26701 attccgggtt ttcgctggta cgcagcagtt tcatgcccag cactttggta taaaaatcga
    26761 tggagcgttg caaatcgcca acgcgcagca tggtatgaag aagacgcatc tttttatcct
    26821 caaaatggct aacagattca aaatgttgtt ttagtatagc ggcggcttta cgccgctcgc
    26881 aatgttggat tacaacgtcg ggtaatcagt gtagccttcc gcaccgccac catagaaact
    26941 ttcagcacgc tgtgggttaa gctcagcttt gcgctgcaag cgggcgacca gatccgggtt
    27001 cgcaatccag tcacgaccaa atgccaccgc atcaattaac cctttgccga tcagcgtttc
    27061 agctttttct actgtgtatg cacctgcgcc gataatcgga ccgtggaaac gggcgcgtac
    27121 tttttcgcgg aacgcatcag tatacggttc accccccgcc caatctggtt ctgacatatg
    27181 cagataagca atgccgcgtt tacccagttg ttcaatcaga tacagtgcat cggcttcttc
    27241 attcgggccg ttatccgtgt tctggaaagt accgattggc gaaacgcgaa tgccaatgcg
    27301 atcggcaccc cattcttcaa tcccggcatc gaccacttcc agtaccaaac gtgcgcgatt
    27361 ttccacgctg ccgccgtact gatcggtacg atggtttgaa gaaggggaaa ggaactgatg
    27421 cagcaaataa ccgtgagcag agtggagctc taccagatca aaaccggctt cacgcgcgtt
    27481 agcaatggcc tgacggaaat cattgacgat acctggaatc tcttccagtt caagcgcacg
    27541 cggcatggat gtttcaacac ggatcgcctg accattttca tcgcgcagag aagtacgtgt
    27601 tcccgcgcta agtgctgaag gcgctaccgg tgcctgaccg ccaggttgca ggctggcgtg
    27661 agaaatgcgt ccggtgtgcc acagctggac ggccatatga ccattttcag catgaacgcc
    27721 agcggtaatt tttttccatg cggcaatttg ttccggacta tggatgccag gtgcaccagc
    27781 ataccctttt gcctgggcag aaatttgcgt ggcttcgcta ataatcaaac cggcactggc
    27841 acgttggcga tagtattccg ccatcaacgg ggtagggatg tcacccggtt caatactgcg
    27901 cagacgcgtc agcggtgcca taaaaatacg gtttgccgca gttattgcgc ccactttcag
    27961 tggggaatac agtttttcag atgacataaa ggctcccggt cgtctggtga taaaaaatgc
    28021 ctgctaaacg gcaggcgtcg caataatggt ttttacatgg gccagcgcgt tttccagtgg
    28081 ctcggaattg cgcgaaattt tggcctgcag attcgcgcca agccatagtg cgtaaagcac
    28141 ttgtgcctgt tgtagcggtt cgccacaaaa ggttaaacaa tggttctcac ggccattttc
    28201 cagagcctgc gagagcaggg cgatcacgcc gcgagcacct ttatccatcg cgctgcgcat
    28261 atcttctgac agatcgcaca cttcggcaga gagttttact gtcaggcaac cactgatggt
    28321 tccatgttgg caaaactggt tcagtgtttg ctggtaataa gccagtatgc ggtcgcggta
    28381 gttaccttcg ccggattgca gcaactcagt cagtcgctgg tgatatgcgg cgtaatgacg
    28441 ctcaagcatg gcaacgccaa acgcttcttt agagcgaaag tagtgataga aggacccttt
    28501 cggcacttca gcggttttta gtaattcgct tagccccatc ccggtgaatc cacgttgcag
    28561 gcaaagttgc tcgcccgtcg ccaggagatg ttcgcgagta tcatgttcgg tgtgtttgtt
    28621 catgcgttgg agtgtagtag accagtcggt ctagtgcaag caaagttgct actttggaca
    28681 ttaacagctt caataatggt aataatctta tctgaggagg gcgaggtggc ggagcaatta
    28741 gagttctttc ccgtccagag cccgtgccgg ggaatttgcc agtctgacga acgcgggttt
    28801 tgtcgcggct gttttcgtag tcgggatgag cgttttaact ggaataaaat gagcgatggc
    28861 gaaaaacaag aggtgctgcg cctttgtcgg cagcgattaa tgcgcaaatt acgcgcaaat
    28921 aagccagcgt catcagacga accggagcaa ccatcactct tttgaaccta atcttgcgta
    28981 tactcctctc atatctgata agaggaagcg attatggttc agcgtattac tattgcgccg
    29041 caaggcccgg agttttcccg ttttgtgatg ggctactggc gattgatgga ctggaatatg
    29101 tccgcccgcc agctggtcag ttttattgaa gagcatctgg atctcggcgt gaccaccgtg
    29161 gaccatgctg atatttatgg tggttatcag tgcgaagcgg cgtttggcga ggcgctgaaa
    29221 ctggcacctc acctgcgtga acggatggaa atcgtcagta aatgcggtat cgcgacgacc
    29281 gcgcgtgaag aaaacgtcat tggtcattac atcactgacc gcgatcacat cattaagagc
    29341 gccgaacagt cgctaattaa tcttgcgacc gatcatctgg atctgctgtt aatccaccgc
    29401 ccggacccgt taatggatgc cgatgaagtg gcggacgcgt tcaaacatct gcatcagagc
    29461 ggcaaagtgc gtcattttgg cgtatcgaac tttacgcctg cgcaatttgc cctgttgcaa
    29521 tcacgtctgc cgtttaccct tgccactaat caggtggaaa tatccccggt acatcagccg
    29581 ttactgctgg atggcacgct cgaccaactg caacaactgc gtgttcgtcc gatggcgtgg
    29641 tcctgccttg gtggtggtcg tctgtttaat gatgattatt tccagccgct gcgtgatgaa
    29701 ctggctgtgg tggcggagga gttaaacgcg ggctcgattg aacaggtggt ttacgcctgg
    29761 gtattacgtt taccgtcgca gccgctgcca attatcggct caggtaaaat tgagcgcgta
    29821 cgggcagctg tcgaagcaga aacactgaaa atgacccgtc aacaatggtt tcgtatccgt
    29881 aaagcggcac tggggtacga cgtaccgtaa gcacttttag gaatagccgc cgttcaaaaa
    29941 tgtgtcactg gtttacactt attcaggaat gcacaatgaa cggaggtcct atgaaacgtt
    30001 ttagtctggc cattctggcg ctggttgttg cgaccggcgc acaagctgcc agtgaaaaag
    30061 tcgagatgaa cctcgtcacg tcgcaagggg ttgggcagtc aattggtagc gtcaccatta
    30121 ctgaaaccga taaaggtctg gagttttcgc ccgatctgaa agcattaccc cccggtgaac
    30181 atggcttcca tattcatgcc aaaggaagct gccagccagc caccaaagat ggcaaagcca
    30241 gcgccgcgga atccgcaggc gggcatcttg atccacaaaa taccggtaaa catgaagggc
    30301 cagaaggtgc cgggcattta ggcgatctgc ctgcactggt cgtcaataat gacggcaaag
    30361 ctaccgatgc cgtcatcgcg cctcgtctga aatcactgga tgaaatcaaa gacaaagcgc
    30421 tgatggtcca cgttggcggc gataatatgt ccgatcaacc taaaccgctg ggcggtggcg
    30481 gtgaacgcta tgcctgtggt gtaattaagt aattaagagg ccagcgtacc ttgcggtggt
    30541 gcttgctcaa gttgcgaaag cgagcagtac agccgccaga caattgccgc cagctcacgg
    30601 gcggcaggtt gatgatgacg ggcaaggctg tcgcaaatcc gctgtaattc ttcaagtgtg
    30661 gccgccagtg atttttgctg aacgccacgc tcactcatca ctccccgcaa cagtgaaata
    30721 cagttatccc gtactcgcga taacggatcg gaacgcgatt cccagtcgcg taattgccag
    30781 acaacatgag aacagttcag cagcactaca ccccagcgta ataaccaacg gcgagccagc
    30841 gcatcctggc tgttactcaa ctgactgacg tgatgataag tgagcgattc aaattcgctt
    30901 tcactcagtg ttggatggcg gcttagttga tcgacaaaat cccggcgcag cgcgcgaata
    30961 tggcggcggc ttttgcgagc atccgatcct ggacgcagaa tggcgaacgc taaccacgcc
    31021 aacgcgacgc caacgatttt tgccagatta tcgttaagaa aatcagcaaa atcatacacc
    31081 gggggattag tgacagcgat aaaagaaccc ataaaaacaa tcagttgccc ccacaatgcg
    31141 gcaaatttgg gcatctgcaa tttaagaagc tgcattgtcg ccagcagtgg aaagagaaac
    31201 agtaaaaatt gccacagatc gctaatctgg accatcagac caaatttgac cacaaagctg
    31261 aatagcgaaa gtaacaccag cgtgcgcatc agcagtgaca acgacttaaa cggtgctgcg
    31321 acggcggagt agagtacgca gctaattgct gccagcgtta atgcattggc accggcatcc
    31381 cattgcgaag caatactcca tgcgccaatc atcatcaacg tacaaaatgt acgcagcccg
    31441 ctccacatag cttcggcgtt atcggtatga cgagccaggc cggatgtccg tgggagtctg
    31501 gtatgatcat ctacaccgct ttgcagacga tgtaattcct gactactttg cagataaagg
    31561 cggcaaaaat aacgtagtcg ctgccagaag gcgacgtgcc gatagtcggc gacgttggtc
    31621 gggcgtagcg gggcgataat acgtgcgacg gtgtaaacat ctgtttgcga actggcgagc
    31681 gccgtcagca actgttcgag aatttctcgt gtggcacctg gcggtgaggg ccagttgagc
    31741 aacatacgtc gcaggctgga gatgacactg gtcatacgta attgctggtg gagcagcgca
    31801 ttaaggcgcg cgttttgctg gcgaaaacga tagtggctcc agaaagcctg gatacgcagc
    31861 aaattcatgg tcagtatctg cccaatcacc ccttcatgtg ctgcacgaat ggcatcggtt
    31921 gtttcaggct gccagagtaa actggcatgt tcaagtagtc gggcgtgcat gtttttcaat
    31981 gcggttaaaa gggctgtagc atcggaactg ctaggcagga tcatcatcat catgccgccg
    32041 cacaaaatgc cgacaatcac ctcgcaaacg cgcgcctgag cgatatccca cagctggctg
    32101 gcctcagtaa tattaaccat cggaaaggcg atgatggcag ccgtgtagcc cgccagttga
    32161 aatgcatacg cgacgttatt cgtgaagtgc gcacaggccc aggtacaaaa gccaagccac
    32221 gccgacatgc tcaatagaaa aaaccacggc tcattgagcg tatgcccggc aagaagcagt
    32281 gccgcaatgg ctccgagcaa actgccagcg atgcgtccga ggcttttgct gataacaccg
    32341 ccaacggtgg gaaagctaac cactgcagcc gaggtcatcg cccaataggg ttcatccaga
    32401 tttaaataat aggcaaccgt cagcgccaga cacatggcaa tggtattgcg taacgcataa
    32461 cgccattgcg ccagcgtggc cctgaaccag ggcaaattgc gcaaggacca tgacgatgcg
    32521 ttcattattg acctacggca atgctacagg tggtgcccga caccagcgtg acatcgcctg
    32581 ggactttatc cagcgtaaag cggacgggaa cacgttgggc gaggcgaacc cagggaacgt
    32641 taggtttcac atccggaata aggcttgagt cactttcaac gctctgatca taaatcgccc
    32701 gaccgatact ggaaacgtga ccctgtaacg ttttgttatc actatagagg gtaatttgtg
    32761 ctggtgcacc ttcgcggata tggcgcaact tggtttcttc aaaataacca atgacgtaaa
    32821 acgagtggct gtcgacaagg gcaaacagcg gtttcccggt gtcagcgtaa tcgccgatgc
    32881 gggtagtgag attagtcacc catccactca ccggggcgcg aatttccgtt tgcgccagtt
    32941 gccattgtgt ctgtttcagc gtcgcctgtg cggcatcaac actggcctgc atcgctttaa
    33001 cattgaggtt agcggtatcg agttcttcgg cagaaataaa attttgtgag agatgacgac
    33061 gacgattcgc ttcgttattg gcttttgcga ggtcagattg cgctttcgcc agctgagcct
    33121 gtgcattcag cttggcgatc tgaaagggcg ttttatcgat cgtgagcaga agatcccctg
    33181 cgttaaccag ctggttgtct tttatattca gctcaacaat acgtcccgac acctgtgggg
    33241 tgatagaaac ctgttctgcg cgtattttcc cgtcgcgtgt ccacggagat tgcatgtaat
    33301 aattccacag ccaccatccg gcaagaaccg aaactatcgc tacaatgatt gttgagaaat
    33361 acttaattgt tttaattgac atagttacca cgcaatcagt attgccagag caaggcaaac
    33421 gcaaatcgca aacagcgata aatccattaa caagggatgc cagatgtcac cggcgtagat
    33481 ccagccacga agcaggcggt gtacgacaag ccagatgacg aatccaaacg cgaaggcttt
    33541 gaaaaacgga ggaaagtaga cggacgcacc gaacaccaga tcttgtaagg gcaatcctgt
    33601 tgcattgagc ataaacttca caaagtagat tcctttacga ccgaatatat tgcgttagat
    33661 taataaatat tctttaagtg cgaaaaattt acgcgcaatt tctgaaaaat atgtctgacg
    33721 gtaaccaaat gcagcaatac atttgtttta gcaatacaat tgctgcacac tattctaaaa
    33781 gccgcataat atcttagcaa gctaattata aggagatgaa attggaatcg ccactaggtt
    33841 ctgatctggc acggttggtg cgcatatggc gtgctctgat agaccatcgc ctgaaaccgc
    33901 tggagttaac acaaacccat tgggttacgt tacacaatat ccatcagtta cctccagacc
    33961 agtcgcaaat tcaactggca aaagcgattg gcatcgagca gccatcactg gtccgtactc
    34021 tggaccaact ggaagaaaaa gggttaattt cgcgtcaaac ttgtgccagc gatcgtcggg
    34081 ctaaacgtat taaactgacg gaaaaggcag agccactgat cagcgaaatg gaagctgtta
    34141 ttaacaaaac ccgcgcggaa attttacatg gcatctccgc agaggaactg gaacaactga
    34201 ttacgctcat cgcaaaactc gagcataata tcattgagtt acaggccaaa gggtgaaatg
    34261 aagggggcgt gtggtcagta acctgaccac acgcaaacta tttagcgcgg agaaacggtc
    34321 acctgactgc cattgctggc cagtacgaca cgttggcccg gagagaaacg agtgttgcct
    34381 tgtttctgta ccaccatgat ggtattacca tcgtctttac gaatttccag ctcgacaccc
    34441 tgcgttttgt tcattgcact ctgtacgccc tgaccagcta cgccacctgc aacagcgcct
    34501 gctgcagtag ccagagaacg cccggttccg cctccaacag tatttcccag gaaaccacca
    34561 agaacagcac cgccaattgc accgataacg ttggaatcat caccgccctg aatctgtacc
    34621 ggacgtacgt taacgatggt tccatagctg acattctgta cttgtttcgc ttcagaagcg
    34681 gtataaacat cccctgacag ggtgtcgtta ttaacacaac cgacaagaga cagacctacc
    34741 attgaaacaa ccaatacgcg tttaatcatt gaaaaatctc ctgttcacca tgaaacgcca
    34801 cgcaagcatc cctcatggtc aaagtatatg gcatatgtga atccatggtt acatgttcta
    34861 ccaaatatga gcaaatcata atgaaagtag gatcaaccaa ttataaacaa aacattcatg
    34921 atgattattg atttgaagat aatgttttac taccaacggt aaaaatcggt cattccgcat
    34981 ggtattcacg aaaagtgtat gtttgagtgt gcgcaaacgt cagattgaaa aaggatgaac
    35041 tatgaaatcg ggccgcttta ttggcattat gtcaggcacc agccttgatg gtgttgatgt
    35101 tgtgttggcg acaattgatg aacaccgggt cgcacagctg gcaagtttga gctggccgat
    35161 cccggtgtct ctgaaacagg ctgtactgga tatttgccag ggccagcagc ttacactttc
    35221 gcagtttgga cagcttgata ctcaactcgg gcgacttttt gctgatgcgg tcaatgcctt
    35281 gcttaaggaa caaaacctgc aggcgagaga tatagttgcg atcggctgtc acggtcaaac
    35341 cgtctggcat gaaccgacgg gcgtggcacc acacacttta cagattggcg ataacaatca
    35401 aattgtggca cgcaccggaa ttacggttgt cggtgatttt cgccgtcgcg atattgcctt
    35461 gggaggacaa ggcgcaccgc tggtacctgc gttccatcat gccttgctgg ctcacccaac
    35521 cgagcgacga atggtgctca atattggcgg catcgccaat ctgtcactgc tcattcctgg
    35581 gcagccggtt ggcggctacg ataccggtcc tggtaacatg ctgatggatg cctggatctg
    35641 gcgtcaggcc ggtaaacctt acgataaaga tgccgagtgg gcacgggcgg gtaaagttat
    35701 tctcccactg ctgcaaaata tgctcagcga cccatatttc tcgcaacctg caccgaaaag
    35761 caccggacgc gaatacttta actacggctg gctggagcgc catttacgcc attttccggg
    35821 tgttgatccc cgtgatgttc aggccacact ggcagaactc accgccgtga ccatttctga
    35881 acaagttttg ttgagcggtg gctgcgaacg attgatggta tgtggtggag gtagtcgtaa
    35941 tccgctactc atggcgcgtc tggcggcatt actgccaggc acagaagtca ccaccaccga
    36001 tgccgttggc attagtggcg atgacatgga agcattggct ttcgcctggc ttgcctggcg
    36061 gacgctggcg ggattaccag gaaatctgcc ttccgtcact ggcgcaagcc aggagacggt
    36121 actgggggct attttccccg ctaacccgtg atatcagagt taactgaatt tttcttaccg
    36181 cgtctgccgt tacacttgga acgttaggga gggcgtattg ctctccagac caggaaagtc
    36241 ttcgggatat gactatgaaa aaactgttaa ttatcatttt gccggtcctt ctctccggct
    36301 gtagcgcctt taatcagctg gttgagcgca tgcaaaccga tacgctggaa taccagtgtg
    36361 atgaaaaacc gttgacggtc aaactgaata atccgcgcca ggaggtcagt tttgtttacg
    36421 ataatcaact actgcatctc aaacagggca tttcagcctc tggcgcacgt tacactgacg
    36481 gaatctatgt tttctggtcg aaaggcgatg aagcgactgt ctataaacgc gaccgcatcg
    36541 tcttgaataa ctgtcagtta caaaatccac agcgttgaga tttttccagg ggcggcgcac
    36601 aatagcgtca cccactgaca atccgtaaag aaaaccatgt ctgataacga cgaattgcag
    36661 caaatcgcgc atctgcgccg tgaatacacc aaaggcgggt tacgccgacg cgatcttccc
    36721 gccgatccat taaccctttt tgaacgttgg ctctctcagg cttgtgaagc caaactggcg
    36781 gaccctaccg cgatggtggt cgctaccgtg gatgaacatg gtcagcctta tcagcgcatc
    36841 gttttactca aacattacga cgaaaaaggc atggtgtttt acaccaacct cggcagccgt
    36901 aaagcacatc aaatcgaaaa taatccgcgc gttagcctgc tgttcccgtg gcataccctt
    36961 gagcgccagg tgatggtgat cggtaaagca gaacgacttt cgactctcga agtgatgaaa
    37021 tattttcata gccgcccgcg tgatagccag attggtgcat gggtttcgaa gcagtccagt
    37081 cgcatttctg cccgcggtat ccttgaaagt aaattcctgg agctgaagca gaagtttcaa
    37141 cagggcgaag tgccattgcc gagcttttgg ggcggttttc gcgtcagcct tgaacagatt
    37201 gagttctggc agggtggtga gcatcgcctg catgaccgct ttttgtacca gcgtgaaaat
    37261 gatgcgtgga agattgatcg tcttgcaccc tgaaaagatg caaaaatctt gctttaatcg
    37321 ctggtactcc tgattctggc actttattct atgtctcttt cgcatctggc gaaaagtcgt
    37381 gtaccggcaa aggtgcagtc gttatataca tggagatttt gatggcaagc agtaacttga
    37441 ttaaacaatt gcaagagcgg gggctggtag cccaggtgac ggacgaggaa gcgttagcag
    37501 agcgactggc gcaaggcccg atcgcgctct attgcggctt cgatcctacc gctgacagct
    37561 tgcatttggg gcatcttgtt ccattgttat gcctgaaacg cttccagcag gcgggccaca
    37621 agccggttgc gctggtaggc ggcgcgacgg gtctgattgg cgacccgagc ttcaaagctg
    37681 ccgagcgtaa gctgaacacc gaagaaactg ttcaggagtg ggtggacaaa atccgcaagc
    37741 aggttgcccc gttcctcgat ttcgactgtg gagaaaactc tgctatcgcg gcgaacaact
    37801 atgactggtt cggcaatatg aatgtgctga ccttcctgcg cgatattggc aaacacttct
    37861 ccgttaacca gatgatcaac aaagaagcgg ttaagcagcg tctcaaccgt gaagatcagg
    37921 ggatttcgtt cactgagttt tcctacaacc tgttgcaggg ttatgacttc gcctgtctga
    37981 acaaacagta cggtgtggtg ctgcaaattg gtggttctga ccagtggggt aacatcactt
    38041 ctggtatcga cctgacccgt cgtctgcatc agaatcaggt gtttggcctg accgttccgc
    38101 tgatcaccaa agcagacggc accaaatttg gtaaaactga aggcggcgca gtctggttgg
    38161 atccgaagaa aaccagcccg tacaaattct accagttctg gatcaacact gcggatgccg
    38221 acgtttaccg cttcctgaag ttcttcacct ttatgagcat tgaagagatc aacgccctgg
    38281 aagaagaaga taaaaacagc ggtaaagcac cgcgcgccca gtatgtactg gcggagcagg
    38341 tgacccgtct ggttcacggt gaagaaggtt tgcaggcggc aaaacgtatt accgaatgcc
    38401 tgttcagcgg ttccttgagt gcgctgagtg aagcggactt cgaacagctg gcgcaggacg
    38461 gcgtaccgat ggttgagatg gaaaagggcg cagacctgat gcaggcgctg gtcgattctg
    38521 aactgcaacc ttcccgtggt caggcgcgta aaactatcgc ctccaatgcc atcaccatta
    38581 acggtgaaaa acagtccgat cctgaatact tctttaaaga agaagatcgt ctgtttggtc
    38641 gttttacctt actgcgtcgc ggcaaaaaga attactgtct gatttgctgg aaataatgca
    38701 ttaagtggaa agggggagtg agaaatcact ccccctggtt tttatacagg gaacatgatg
    38761 aaaaatattc tcgctatcca gtctcacgtt gtttatggtc atgcgggtaa cagtgcggca
    38821 gagtttccga tgcgccgcct gggcgcgaac gtctggccgc tgaacaccgt tcaattttct
    38881 aatcacaccc aatacggcaa atggactggc tgcgtgatgc ctcccagcca tttaaccgaa
    38941 attgtgcaag gcattgccgc cattgataaa ttacacacct gtgatgccgt attaagtggc
    39001 tatctgggat cggcggagca gggtgaacat atcctcggta tcgtccgtca ggtgaaagca
    39061 gcgaatccgc aggcgaaata tttttgcgat ccggtaatgg gtcatccgga aaaaggctgt
    39121 atcgttgcac cgggtgtcgc agagtttcat gtgcggcacg gtttgcctgc cagcgatatc
    39181 attgcgccga atcttgttga gctggaaata ctctgtgagc atccggtaaa taacgtcgaa
    39241 gaagcggttc tggcagcgcg cgaactcatt gcgcaagggc cacaaattgt attggttaaa
    39301 cacctggcgc gagctggcta cagccgtgac cgttttgaaa tgttgctggt caccgccgat
    39361 gaagcctggc atatcagccg tccactggtg gattttggta tgcgccagcc ggtaggtgtt
    39421 ggtgatgtga cgagcggttt actgctggtg aaactgcttc agggggcaac gctgcaggag
    39481 gccctggaac atgtgaccgc tgcagtatac gaaatcatgg tgaccaccaa agcaatgcag
    39541 gaatatgagc tgcaagtggt ggctgctcag gatcgtattg ccaaaccaga acattacttc
    39601 agcgcaacaa agctctgaat tatctcattt cggcccacaa cgatctgtgg gccgatggct
    39661 attactttaa gccttccgct gacagcgcgt cttgtacttc cggacgttca gccatacgtt
    39721 gcataaatgc cgcaatgtgt tctaaccctt ccagattcag tttcaccgcg tatgcccagc
    39781 gcagaaccgt aaacagatag gcatcagcaa ttgtaaatct ttgcccgcag atccagtgct
    39841 catccttcag tgcttcgttc acatattgca gcttcttctc cagttgcgca cgaactgtcg
    39901 gtttgtactc ttccggtgta tcagggcgaa acagaggtgt gaaaccttta tgcagctcgg
    39961 tggcgatgta attcagccat tcgatggttt tatagcggga aatactgttt accggtgcca
    40021 gcaactggcg gtcggggacg ctgtcggcaa gatactgcat aatcgcaacg ccttccgtca
    40081 gcaaagtacc atcgtccagc agcaatgctg gcacctgtcc cttagggtta acggcaaagt
    40141 aatcgtcacc gttttcgaga cgctttttca ttaaatccac actgacgagc gtaaaatcct
    40201 ttccgctctc acgcagggtg atatgggaag cgagagagca ggcgcccggt ttgtagaaca
    40261 atttcatcgg taactccttt tgtctgtggt ttaaagtatg gtagtgcgtt gctgaacaaa
    40321 aaaaaagccg ctaacgaaaa gttagcggct tgtaaaagag tttccctgaa ttttacgcta
    40381 cggctgcttt cgccgctttg tctgcagcgt catcctgcgt catgcggtgc agtttcggcg
    40441 cggtcagcag catcagtact gcaataacgg cagtagcgac accaatctgc aagaatacgc
    40501 gaccatagac ttccagtgac atcagcggat cggtaacgtt atccggcaca gccatcatac
    40561 ccgcaacata accaccaatc aggtttgcac cggcagtggt caggaaccag ctacccataa
    40621 tgaagcccat cagacgctgc ggaacgagtt gtgcaaccat tgccagaccc agaccagaga
    40681 tcatcagttc cccgatactc tgcaggccat agcttgcgac cagccagctt acagacacga
    40741 tacctgcgtc agacgcgaat tttgcaccca gcggcagaat caggaacgca ccagaacaca
    40801 tcaccatacc gattgcaaac ttagttggca tcggcagagt atcgcccatc ttgttataga
    40861 tagcggccag aatcgggcta ccaatgatga tccagaacgg gttcagtgcc tgatactgct
    40921 caggttctac ggccaggccg agaatggtgt gttcaacgtt acgaatcgca aagaagttca
    40981 gtgacgttgg catctggctg tacagcacga agaagataat ggcttcgagc atcaggatga
    41041 aggcaacgat cattttacga cgcgcagcac ctttcatcgc gaaggcttct ttaccgaaga
    41101 taaccacgat accaaaggca acaacgccca gcgccatacg cgcaacttcc tgattgtgca
    41161 gcagccaggt ggcgatagcg atcagtgcca caacaccaat aatggtcagc agcaggttac
    41221 ggtagttgat aggctcgaag tctggttttg aaccgtactg tttaacccag cgttggcaga
    41281 aggcgaagtt aacgatagtg atcagcaggc ctacaacgct caacgcaaac gcaacactcc
    41341 agccatattt cgcggccagc cacggggtgg cgatcataga gaagaaagag ccgatgttga
    41401 cggacatgta gtacatggtg aatgcaccgt ccagacgcgg gtcgtttttc tcatagcatg
    41461 tagaaagcag agaagacggg ttagctttaa acaggccgtt accgaccgca atagccgcca
    41521 tacccatata aacgataccg gcgtcgtgac cagaccaggc aaccagagca taaccaatcg
    41581 ccagcacaat agcgccgagc ataattacgc gtttagtacc cagtaccttg tcacctaacc
    41641 agccgccgat agcgaccaga ccataaacca gggcactaaa ggaagagaaa agggtgattg
    41701 aatccgcttc agacataccc agttgtttaa ccaggtaaac agccataatt ccttgtaggc
    41761 cgtaataacc aaaacgttcc cataactcaa tcgagaagat gagatagaac gccttcggtt
    41821 gtttgaaagc gttcaaactg acgctttcag ttggtttttg gtttgcagtg gacactttta
    41881 cctctttttt tacatcccat attaacgggg gtgctttctc gcgttatgtc taaaaaacat
    41941 acgcgcaatt gttatagtgg gaggggaaac ggcaggtaat gttcactatc ttgcactttc
    42001 agacaagtcc tttgtaataa tctgttacat ataactgagg cggggaattc aaccattccg
    42061 ggaaatgtta tccagcgtta aatttttcaa aaaacgtaac ggcagatttt ttcactgcat
    42121 tgagaggggg atgatggctt gacggcgata tgttctgcta tttagcgttt ttcctagtga
    42181 gatagtccag tttctgaaaa atagccagtg taatgttttg taggtcaata tttgttgcgt
    42241 gtgtaaattt aaatgatttt aacctgattc ttgcaatgtt tttacattgc cacagcatcg
    42301 tgatcttgat cacgaatgaa tagaaaattt cgctattaaa aaaacaatta acctgcattt
    42361 ttggttatcc gcaaacggat tacctgatga taacgctaag caatggcatc attgacgtta
    42421 aaaagatgtt ttaaaagacg ataaagaaag gttatcaatg gggtaatcgg tgttacccct
    42481 tttcttcaga tgtcaacttt ctctttgtat tcacaaagat cttcaataat acaagagcca
    42541 cagcggggct tgcgggcaat gcaggtataa cgcccgtgca ggatcaacca atggtggcag
    42601 tcgactttaa actctgccgg aaccactttc agtagctttt cttctacctg ttcgacgttt
    42661 ttccccggcg caaattgagt acgattacaa acgcggaaaa tgtgcgtgtc gacagcaata
    42721 gtcggccagc cgaatgcagt gtttaatacg acgttggctg ttttacgacc tacgccgggc
    42781 agggcttcaa gcgcagcacg atcttccgga acctcgccat tatgctgatc cagtaagata
    42841 cggcaggttt tgatgatatt ttctgctttg ctgttataaa gcccaatcgt tttgatatag
    42901 gttttcaccc cttcaacgcc cagttcaagc atcgctgcag gcgtattcgc caccgggtag
    42961 agtttcgccg tcgccttatt aacactgaca tcggtcgcct gagcggaaag cagtacggca
    43021 atcagcaatt caaaaggcga actgaaatta agctcggtgg tgggatgagg attgttctca
    43081 cgcaggcgag tgaggatctc caggcgtttt gctttgttca tcagacattc cctgtttcac
    43141 cgtttggcaa tgcacgttcg gcagctgctt cagtacggcg ctttttcatt ctttcatcaa
    43201 tcaggtattt tcctgccagc atcagtccca ggccaataaa tgcacctggt ggcagcatcg
    43261 ccaacaggaa aggggagtcg gtgtggaaaa tctccacgcg taatactttt acccagctac
    43321 ctaacagcgc atctgcaccg tcaaacaatg tgccattgcc gataatttcg cgtagtgaac
    43381 ccagcacgaa catggcgcag gttgcgccca taccaattga aaagccgtcc agtgccgaaa
    43441 gcgctggacc ttttttggcg gcgaaggctt cagcgcggcc cacaacgata cagttagtga
    43501 caatcagcgg aataaaaatc cctaatgatt gatacaggcc aaaggcgtag gcgttgatca
    43561 gcatctgtac agcgctgacc accgaggcga tgatcatcac gtaaatggga atgcggatct
    43621 cggctggcgt ccagtgacgc agcgtcgaaa tggtcaggtt ggtcagcgtc agtaccagcg
    43681 tagtcgcaag tcctaaaccc agagcgttag tggcagtgga cgtgaccgcc aacagaggac
    43741 aaaggccgag caactggacc agcgcagagt tgtttttcca caacccctga acaataacgt
    43801 ctttaatttc gctcacgctt tattctccac aggcaggaag ttgagaaagt tgtgccggta
    43861 acgtctgagc gtacaatccg gcgcgtttta ccgcattaac caccgcgcgg ggagtaatcg
    43921 ttgcgccggt gaactggtcg aaatcaccac catctttctt caccgcccag tgcgcatcat
    43981 ccgcaccact gattttttta cccgcaaaat gggtgatcca gtcagaaagg cgcagttcga
    44041 ttttatcgcc aagccctggc gtttcgtggt gctctgtcac gcgcgtgcca agtaccgtgc
    44101 cgttaaaatc ggctcccacc agcagctgaa tcgcaccgga atagccatct ggcgcggttg
    44161 cttccagaac ggcggctacc ggtttgtcat cctgtttggc gatgtaaacc cgatgctcac
    44221 ctttacctaa ctctggcgca gttaccagat agcaactctg tgccagcgca ttgttatagc
    44281 gttcggctgg cagcacctga tcaaataacg ccttttgttg cagactggcc tgttcagcaa
    44341 tcgtcgtttt ggtcatctgg ttgatggccg cagttaaccc tgttgaaccc gctgcaaata
    44401 gcgctaacgt aatgccgtgt tttcggatag ttttcagcat ggtttaccct ttgcgatggc
    44461 cgtagacgcg cggacgcgtg tagtaatcga tcagaggaac cgtgatgttc gccagcagga
    44521 cggcaaaagc cacgccgtca ggatagccgc cgaaactgcg gatcaaccag accaataagc
    44581 ccgcaagcgc gccgaaaata agacgaccac gattggtcgt agaagcggta accgggtcag
    44641 tcaaaataaa gaatgcgccg agcatggtcg ctccagacag cagatgaatt tgcggtgctg
    44701 ccagtgtttc tggtgagaac aaccagccca acgttgcgca tagcgtcagc gttactaaga
    44761 agctgagggg aatatgccag cgaatcgctt tctgccatag caaccacacg ccgccagcca
    44821 gccaggcgag atttacccat tgccaaccag cgcccgccag aataccgctg tagatcggat
    44881 attgcataat ctgttcaacc gaatgaccgg cacggacaga ggttttaaat gtatccagcg
    44941 gtgtcgcctg actaatgcca tcaataccta agcgtagtgt gttcatatca ccaccactgg
    45001 cggtatgccc gctgaaaata acctgtatgg cgtcgataaa accagggatg ttgaccgcaa
    45061 tttcatgtgg cggtaaccag ctggtcatct gcaccgggaa ggagatcagt aagaccacat
    45121 aaccaatcat tgccggatta aacgggtttt gtcccagacc gccatataac tgtttagcga
    45181 tgatcaccgc aaacaccgta cccagcacga ccatccacca aggcgcgagg gggggaatac
    45241 ttaccgccag caataagcct gtcagcaatg ctgagttatc tttcaacgtg gcggctaccg
    45301 actgtttgcg tagtttgagt acgagagctt cggctaacag agcactgacc gacgccaaca
    45361 ggatctgaac gagagtaccc caaccaaaaa accacagttg cgctgcgatt cctggcacgg
    45421 ctgcgagcaa caccaacagc ataatgcgcg atgtctggcg ctggttatgg gtataagggg
    45481 agctagctat tctgaatacc atttagtcct cgtttacaac cttttgctgg gcggcttttt
    45541 tggcctgaac gcgggcaata gccgcggcaa ctgccgcttt gcgcggatca acctgttctt
    45601 ctggctccgc attagcctgt tgctgttcca gtttgcg
//
LOCUS       DAPGOF010000016        43228 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.016, whole genome shotgun sequence.
ACCESSION   DAPGOF010000016 DAPGOF010000000
VERSION     DAPGOF010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43228
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.016"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(72..437)
                     /locus_tag="RHV13_000780"
     CDS             complement(72..437)
                     /locus_tag="RHV13_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HDV1240336.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDSRRTSWLESQGWTVLRFWNNEIDCNEVAV
                     LEIILQELNRLSPSPQPSP"
     gene            complement(505..1311)
                     /gene="sapF"
                     /locus_tag="RHV13_000781"
     CDS             complement(505..1311)
                     /gene="sapF"
                     /locus_tag="RHV13_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HDV1240337.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTNLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(1313..2305)
                     /gene="sapD"
                     /locus_tag="RHV13_000782"
     CDS             complement(1313..2305)
                     /gene="sapD"
                     /locus_tag="RHV13_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HDV1240338.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            complement(2305..3195)
                     /gene="sapC"
                     /locus_tag="RHV13_000783"
     CDS             complement(2305..3195)
                     /gene="sapC"
                     /locus_tag="RHV13_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HDV1240339.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            complement(3182..4147)
                     /gene="sapB"
                     /locus_tag="RHV13_000784"
     CDS             complement(3182..4147)
                     /gene="sapB"
                     /locus_tag="RHV13_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HDV1240340.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            complement(4144..5787)
                     /gene="sapA"
                     /locus_tag="RHV13_000785"
     CDS             complement(4144..5787)
                     /gene="sapA"
                     /locus_tag="RHV13_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HDV1240341.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYDPAKSREQLKSLGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            complement(6100..6345)
                     /locus_tag="RHV13_000786"
     CDS             complement(6100..6345)
                     /locus_tag="RHV13_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HDV1240342.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            complement(6479..7864)
                     /gene="puuP"
                     /locus_tag="RHV13_000787"
     CDS             complement(6479..7864)
                     /gene="puuP"
                     /locus_tag="RHV13_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HDV1240343.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            complement(7854..8018)
                     /locus_tag="RHV13_000788"
     CDS             complement(7854..8018)
                     /locus_tag="RHV13_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1240344.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            complement(8167..9585)
                     /gene="puuA"
                     /locus_tag="RHV13_000789"
     CDS             complement(8167..9585)
                     /gene="puuA"
                     /locus_tag="RHV13_000789"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HDV1240345.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            9797..10561
                     /gene="puuD"
                     /locus_tag="RHV13_000790"
     CDS             9797..10561
                     /gene="puuD"
                     /locus_tag="RHV13_000790"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HDV1240346.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            10588..11145
                     /gene="puuR"
                     /locus_tag="RHV13_000791"
     CDS             10588..11145
                     /gene="puuR"
                     /locus_tag="RHV13_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HDV1240347.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            11420..12907
                     /gene="puuC"
                     /locus_tag="RHV13_000792"
     CDS             11420..12907
                     /gene="puuC"
                     /locus_tag="RHV13_000792"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HDV1240348.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEANAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQQAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIQEGEGKGQLLLDGRNAELAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            12909..14189
                     /gene="puuB"
                     /locus_tag="RHV13_000793"
     CDS             12909..14189
                     /gene="puuB"
                     /locus_tag="RHV13_000793"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HDV1240349.1"
                     /translation="MTEHTSSYYAASAHKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLEMLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTATGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            14227..15492
                     /gene="puuE"
                     /locus_tag="RHV13_000794"
     CDS             14227..15492
                     /gene="puuE"
                     /locus_tag="RHV13_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HDV1240350.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPELVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINAFAPLSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMEHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLTNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(15610..16587)
                     /gene="pspF"
                     /locus_tag="RHV13_000795"
     CDS             complement(15610..16587)
                     /gene="pspF"
                     /locus_tag="RHV13_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HDV1240351.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            16754..17422
                     /gene="pspA"
                     /locus_tag="RHV13_000796"
     CDS             16754..17422
                     /gene="pspA"
                     /locus_tag="RHV13_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HDV1240352.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            17476..17700
                     /gene="pspB"
                     /locus_tag="RHV13_000797"
     CDS             17476..17700
                     /gene="pspB"
                     /locus_tag="RHV13_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HDV1240353.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            17700..18059
                     /gene="pspC"
                     /locus_tag="RHV13_000798"
     CDS             17700..18059
                     /gene="pspC"
                     /locus_tag="RHV13_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HDV1240354.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDIPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            18068..18289
                     /gene="pspD"
                     /locus_tag="RHV13_000799"
     CDS             18068..18289
                     /gene="pspD"
                     /locus_tag="RHV13_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HDV1240355.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            18364..18678
                     /gene="pspE"
                     /locus_tag="RHV13_000800"
     CDS             18364..18678
                     /gene="pspE"
                     /locus_tag="RHV13_000800"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HDV1240356.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            18889..20568
                     /gene="ycjM"
                     /locus_tag="RHV13_000801"
     CDS             18889..20568
                     /gene="ycjM"
                     /locus_tag="RHV13_000801"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HDV1240357.1"
                     /translation="MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQHPGFEDFFIAVDP
                     QTDLSAVTRPRALPLLTPFQMRDHSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LAKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIINDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSDNTTWFNFL
                     ASHDGIGLNPLRGLLPESEILELVEALQQEGALVNWKNNPDGTRSPYEINVTYMDALS
                     RRESSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     HSKQITRELNDEATLRHAVYHELSRLITLRRSHNEFYPDNNFTIDTINSSVMRIQRSN
                     ADGNCLTGLFNVSKNIQHVNITNLHGRDLISEVDILGNEITLRPWQVMWIK"
     gene            20582..21874
                     /locus_tag="RHV13_000802"
     CDS             20582..21874
                     /locus_tag="RHV13_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV1240358.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTHVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            21895..22776
                     /locus_tag="RHV13_000803"
     CDS             21895..22776
                     /locus_tag="RHV13_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDV1240359.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            22763..23605
                     /locus_tag="RHV13_000804"
     CDS             22763..23605
                     /locus_tag="RHV13_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDV1240360.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            23636..24688
                     /locus_tag="RHV13_000805"
     CDS             23636..24688
                     /locus_tag="RHV13_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HDV1240361.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCEIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWKLLMNGYLNCEDLIDPVVTFAKSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            24706..25494
                     /locus_tag="RHV13_000806"
     CDS             24706..25494
                     /locus_tag="RHV13_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDV1240362.1"
                     /translation="MKIGTQNQAFFPENIREKFRYIKEMGFDGFEIDGKLLVNNIEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            25504..26559
                     /locus_tag="RHV13_000807"
     CDS             25504..26559
                     /locus_tag="RHV13_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDV1240363.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            26556..28823
                     /gene="ycjT"
                     /locus_tag="RHV13_000808"
     CDS             26556..28823
                     /gene="ycjT"
                     /locus_tag="RHV13_000808"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HDV1240364.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNVTGYTIASRRFVSADQLPLIALEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRSGWQGALFPWESARSGEEETP
                     EFAAINIRTGLQQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            28820..29479
                     /gene="pgmB"
                     /locus_tag="RHV13_000809"
     CDS             28820..29479
                     /gene="pgmB"
                     /locus_tag="RHV13_000809"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDV1240365.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVNAVLPGIRSLL
                     ADLRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTEAQLLLPSTESLTWPRLSAFWQN
                     V"
     gene            29493..30575
                     /locus_tag="RHV13_000810"
     CDS             29493..30575
                     /locus_tag="RHV13_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV1240366.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGYLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQGSLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            30620..31525
                     /gene="ompG"
                     /locus_tag="RHV13_000811"
     CDS             30620..31525
                     /gene="ompG"
                     /locus_tag="RHV13_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HDV1240367.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLEND
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKVAPDWDVKLTDDLRFNGWLAMYKFAN
                     DLNTTGYADTRVETETGLQYTINETVGVRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PITLGNHSITPYTRIGLDRWTNWDWQDDIEREGHDFNRLGLFYGYDFQNGLSVSLEYA
                     FEWEDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(31636..32634)
                     /locus_tag="RHV13_000812"
     CDS             complement(31636..32634)
                     /locus_tag="RHV13_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDV1240368.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHTPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIENGHKNIACLHAPLDVHVSVDRVNGYKQSLEDHNIAVRD
                     EWIVDGGYTHETALQAARQLLGQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLIAGKPSPQNITVATHMTLK"
     gene            32790..34187
                     /locus_tag="RHV13_000813"
     CDS             32790..34187
                     /locus_tag="RHV13_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HDV1240369.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     KGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            34184..35245
                     /locus_tag="RHV13_000814"
     CDS             34184..35245
                     /locus_tag="RHV13_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HDV1240370.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDETQAQNFAPATLDE
                     AQEEEGQVEAVIDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            35393..36934
                     /gene="tyrR"
                     /locus_tag="RHV13_000815"
     CDS             35393..36934
                     /gene="tyrR"
                     /locus_tag="RHV13_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HDV1240371.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(36978..37484)
                     /gene="tpx"
                     /locus_tag="RHV13_000816"
     CDS             complement(36978..37484)
                     /gene="tpx"
                     /locus_tag="RHV13_000816"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HDV1240372.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            37603..38568
                     /gene="ycjG"
                     /locus_tag="RHV13_000817"
     CDS             37603..38568
                     /gene="ycjG"
                     /locus_tag="RHV13_000817"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HDV1240373.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDTTLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAKGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            complement(38543..39271)
                     /gene="mpaA"
                     /locus_tag="RHV13_000818"
     CDS             complement(38543..39271)
                     /gene="mpaA"
                     /locus_tag="RHV13_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HDV1240374.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(39562..40200)
                     /locus_tag="RHV13_000819"
     CDS             complement(39562..40200)
                     /locus_tag="RHV13_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV1240375.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLDYTILRPAWLTDEDIIDYE
                     LTSRNELFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            complement(40221..41150)
                     /locus_tag="RHV13_000820"
     CDS             complement(40221..41150)
                     /locus_tag="RHV13_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV1240376.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYVVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVDAKRIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGALN
                     AMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATK
                     MLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQL
                     VSLPDFSHYELYDKPEAVQEALEKVIPFFNTHL"
     gene            complement(41150..42070)
                     /locus_tag="RHV13_000821"
     CDS             complement(41150..42070)
                     /locus_tag="RHV13_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV1240377.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVDAGSNARTSDISSGEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPTQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            42208..43107
                     /gene="pgrR"
                     /locus_tag="RHV13_000822"
     CDS             42208..43107
                     /gene="pgrR"
                     /locus_tag="RHV13_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HDV1240378.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
BASE COUNT        10643 a        10872 c        11476 g        10237 t
ORIGIN      
        1 ggcgagggga ccatattgtg caaatattgt taccccagca acaaacaggc tcatacagcc
       61 ccctaaccct ttcagggaga gggttggggt gagggtgaca ggcggttcag ttcctgcaga
      121 ataatctcca gcaccgccac ctcattacaa tcaatctcgt tattccagaa acgcagcaca
      181 gtccagccct gcgactcaag ccagctagtg cgcctggaat cataggcaac tgccaaatca
      241 tgctgcccac catccagctc aacgactaca cgcgccgagc agcaagcaaa atcgagaatg
      301 tagctcccca ccggatgttg acggcgaaat ttgaaatcac caaaacgtcg gctgcgaaga
      361 tatcgccaga gctttcgttc ctgcaaagtg agattgcgtc ttaaatcacg cgcatttgat
      421 tttattttat ccatcatctc attctgacaa gatttaatct cttgtcagaa tgagatgaat
      481 aatctggaag gaggattcag aaaattagcg atctttacgc catgcatccg ccgtcaatgc
      541 ctcgccaaaa tgaccggcaa tcagccgttt ggtgagttca tgcagcggcg atgccagcac
      601 atccgcggtg ctgcctcgct cgacaacctc gccctgatgc atcaccagca cctggtcgct
      661 aatgtgcttc atcattccga tatgctgggt aacataaata tacgaaatgc cctgtttttc
      721 ctgtaattcc agcatcagat taatcaactg cgaacgcatc gacatatcca gtgaagcgag
      781 ggcttcatcg gcaataatga cttttgggcg caatatcagc gcgcgcgcca gacccagacg
      841 ctgtttttgc ccgggggcca acatatgcgg atagtaactg acgtgatccg gtagcagccc
      901 aaccatacgc atcgtttcaa taatctgttt gcgacgctgt tccggttcca ggttggtgtt
      961 caggcgcagt ggaaaatcca ggatttgcga gatacgttga cggggattca acgaggtcga
     1021 aggatcctga aaaatcatgc gaatacgctg actgcggaag gaataatcgc caaaatgcag
     1081 tggatgatcg tcaatcaata actcgccgct ggtaggttct atcattcccg ccagcatttt
     1141 tgccagcgtg gatttacccg aaccattctc gccaataatc gccagtgtct ggccttcacg
     1201 tagcgtaaag ctcaagggtt ttaccgcttc tacggtctgg cgacgaaacc aaccggtccg
     1261 gtagcggaac gttttactta aattacgcac ttcaagcagc gtttcgatca tctcactctt
     1321 tctccatgtt cagcgggaaa tgacaggcat agagatgatt tttcgccccc gtcaaacgtg
     1381 gcgtcacaat gcattctcgt tgtgcatatg ggcaacgtgg ccccagacga caaccaatcg
     1441 gtaactgttc aagcagtggg atagcgccgg gtagcgtatt gaggcgactt ttatgcggca
     1501 tcgcgctgcc gaagtctggt atcgcgcgga tcagcgcctg ggtataagga tgatgtggca
     1561 tcgtcaccaa ctccttactc ggcgcggttt ccaccgtttg accgcagtaa agcacgttaa
     1621 ttttatccgc ccattggcta agcatttgta agtcatggct gataagcaaa atagtggtat
     1681 tgctgttttg gttgagacgc gtcagcaggc gaaagatttg cgcctgggtt gttggctcca
     1741 ttgagtttgt cggttcgtca gcaatcagca gacgcggttg attcgccagt gcaatggcta
     1801 tcatcacttt ctgacattca ccttcggtca actcataggg aaaactgcgc atcgcatctt
     1861 tgtgatcttt aatccccacg cggtgcagca gttcaatggc acgccgtttg cgccagccaa
     1921 aacgctgcca ccaacggcct ttataggtcc aggctgggat gttttgcatc aactggcgac
     1981 ccacacgttc tgaaggatct agacacgact gcggttcctg gaaaatcatc gacacgttgt
     2041 ggccaaccag tttgcgccgt tcgcgtgcgg agagacgcag caaatcgata tcatcaaaac
     2101 gcatacggtc agcagtaaca cgccagttat ctttattcac cccacaaatc gctttcgcaa
     2161 tcaaactctt gccggaaccg gattcaccaa caagaccgcg gatttcacct tcggttaagg
     2221 tcatacttac ccggtcgacg gctttaaccc actcatcccc ggttttaaat tcaatagtca
     2281 ggttacgaat atcgagtaat ggcattattc cacccccgca ataattgcac gacggacacc
     2341 gtcgccaagg aggttaacca acaacacgct aatcataatt gccgcacctg gcagcatgac
     2401 agtccacggg gcgacataaa tcagttccag cgcatcaccg agcatcgctc cccattcagg
     2461 cgaggggagt tgtgcgccga gatcgagaaa gcccagcgcg gcgatatcga gaattgccat
     2521 cgacagtgcg cgggtgatct cggtgaccag cccggcggtg atgtttggca tcacagcaaa
     2581 ccagagaata ttcagcgttg atgcgccatc cagacgggcg gcgataacgt actctttttc
     2641 cagttcgtca tgcaccatgc tgtaaatcga acgtaccata cgcggcagca atgccagcca
     2701 gacagcaaac atagcgtggg acaaactcgg tccggcaaac gctacaacga taatggccaa
     2761 cagcagcgaa gggatcgcca gtaaggtatc cagaatatgg ttaagcaccg ctgagcggag
     2821 gccgtgtgtc gccccggcga aggtgcccag taccaggcca cagatcgtcg cggcaagcgt
     2881 caccacaaat gcgccaccca cggttggcgc agctccgctc agtaaacggc ttaaaacatc
     2941 gcgtccgagg tcgtcagtcc ccaggaagaa agaaacttcg ccatagcgcg accatgacgg
     3001 cggcaataat tgataaccga gaaattgctg gtcgatgccg taaggcgcaa accagccgcc
     3061 aaaaatacac agtacagcca gccccgcgca gccgtacagg ccgaccatcg cagaggcgtc
     3121 actataaaat ttgcgccagg cggtacgcag cgtgcccggc gggcgctttt cgctgtatac
     3181 gctatcgtaa ggcataccat tccttatgtt tcagagggtt agccatggca cccaaaatat
     3241 cagaaatcac gttaacaata atgaccagtg agccacacac catcactccg gcggaaatgg
     3301 ctgcgtaatc ctgctggcgg atggcgttaa ttaaccagcg ccctaaaccc ggccagctaa
     3361 aaaccatttc ggtgatcatc gccagcgtca gcatggtgga aaactgtaag cccaggcgag
     3421 gaataaccgg cggtaacgcg ttatgcagaa cgtggcgacg caaaatagta aagcgcgaca
     3481 aaccgcgggt cgccgccgct tttacatagt tctggtcata cacttcgata gtgctgatac
     3541 gcatcaggcg gatcacttca gttgttggcg caaccgacag ggtgatcacg ggcaatatca
     3601 tatggcggat tgcgctcatg atcatttcat cccgccatgg tgagtccgaa agccaggcat
     3661 caatcaacgc aaaaccggta atcggtttca cttcgtagag cagatcgaaa cgccctgaaa
     3721 cgggcaacca acctagcgtg agtgaacaaa acagcgtcaa cagaagcgcc agccagaaca
     3781 ccgggattga aaagcccagt aaggcgatgg cgttgatcaa gttatcctgc cacttatggc
     3841 gcgtaatccc ggcaatcatc cccacgggga tgccaacaat cagcgcaaaa ccgaaggcga
     3901 ggatgcacag ttccatcgtc gccgggaaga cctcttttaa ctgttcggca attggttgac
     3961 cgttaatgct ggaaacacca aaatcccagt ggatcaggcc attaaaccag aacacccagg
     4021 cattccacag tgacgcgcct tgtaacggcg cgtgtggagt gaaatagctc aaactgaagc
     4081 caacgaaggt cagcaggaac aaggtaacaa tcaataacaa aatgcggcgt aaggtgaaga
     4141 taatcatggc tttttcacct catcctgttt ctcgcgatac accccagcaa aggaggcgtt
     4201 accaaacggg ctaagtacca gacctttgat atcgtaccga taggcctgca aacgcaatga
     4261 cgacgccagc ggcaaaatgg gcaattcctg cgccagaata ctctgcgctt cgtcataggc
     4321 ttcaatacgc gctgccagct gctgcgagga gagcgcctta cgcaacacgc tgtcgaattt
     4381 cggatcgcac cagtgggcga ggttggtctg tgaatgaatt gccgcgcagc tcagtaaagg
     4441 acggaagaaa ctgtccgggt cgttactgtc cgtcgcccaa ccggataacg tcagatcatg
     4501 gctcatatcc atcaaccgcg cctcctgaaa gcgaccttct accggcacaa tcaccacttt
     4561 tacgccaacc tgcgccatat ccgcctgaat cagttcggca gttttcagtg gactggggtt
     4621 ccacgcctgc gaacgtgtgg gcacccacag tttcagcgtt aaattttcca gccccaacga
     4681 cttcaactgt tcgcgcgatt tcgccggatc gtattcagta attttagcct cgttgtcata
     4741 ggcccacgag gcgcgcggta aaatagaggc cgccgtttca gccgtaccat aatagatgga
     4801 ttgcatcagg cgctggttat taatcgccag tgccagcgca tggcggacag cgggattatt
     4861 tagcggcggt ttggcggtgt taaatgccag ataggcgacg ttcatcccgg gacgcagcgt
     4921 taaacgcaag cgcgggtcgt cacgcaaaat ggatagctgg ctggcagcag gccaggccag
     4981 aacgtcgcat tcaccagtca ggagtttcga cagacgtccg gtgccgccgg agcctaaatc
     5041 caccaccacc tgcggcatta acggtttacc gcgccagaag tcatcatgac gttgtaggcg
     5101 aataaattgc ccggcgcggt attccgacaa ttgatacgga ccggtgccga ccggttgacg
     5161 gtcgagttgc tcctggcgat cttctttctc taacttccgg gcatattctg ccgacatgac
     5221 cgaagcataa tgggttgcga ggtgccacaa aaaagaagca tccggctggg cgagtcggaa
     5281 ctcaacggta tgattatcca gtttacggac gcttttcacg ttatcggcaa attgcagact
     5341 gtcgaagtag gggaagttgc tgccgttgac gttatgccac gggttgttgc ggtcaaaaat
     5401 tcgctggaag gtaaacacca catcgtcggc attcatttta cgagtgggag taaaccagtc
     5461 ggttttttga aacggaacat cgcgacgcag gtggaagcga taggtcgcgc cgttgtcgag
     5521 tacttcccag ctttcggcaa gttccggcat caggcgatag gtatagggat cgacatccag
     5581 cagtcgatca taaaactggg cggcaagggt atcgacaatt aacccactgc tcgctttgga
     5641 tgggttaaag gtgttgactt gcccgctgac gcaatagaca aaaccgctgt cgcggatatc
     5701 agcatgcggg ggggattcag gcgcggcgat tgcctgacca ctcacaagtc cagcaatcac
     5761 caaaagagac gataatacct ggcgcataat attaagggat tatatgtaaa gaggctatct
     5821 tactaatatt taatgacatt tgccattacc gtttgtgttc aggggtcgta atgagggccg
     5881 tgtggttggt cgttgaacag gtggcgtcgc catctgttca acattcgtac ccgtgatttc
     5941 tctataacta taactcacag aacaacttag cgaggagcag agcggtacga tcgtgagaaa
     6001 cccattaaag cacgctgcag tgcgtgtcat tgttagccgg atgcggcgtg aacgctttat
     6061 ccggacaacg atactgaccg atcgtctgca attaaataat tactcattac cccattgatt
     6121 caaaaactct gcgatctcat caatgcgtac gggattaatc cccgcttcag cagccatttc
     6181 atgttgggct tcttcgctga tctcttcatt gttcatcaaa cgggtgagca gtaactggaa
     6241 gtagtgggcc agcggtgtgc gttcagcctc tgcgacaggc tctgcgcatt cggtcgcgta
     6301 ctcatcggca atatcaaaat attttagcgg gatatcgtgg ttcattattt gcccctgggg
     6361 ttaatgcgct aagcataagg gcggatgata gcactcgtga tccgggtcat acatcttttt
     6421 atagttaatg gcgcggcgcg ttaccctcag gcaggataat gcgccgcgca tccggctatt
     6481 acgtttcgct caccggcgtt ctgtcacctt cgtacagcgg cactttgcga tagcggcgga
     6541 tcagatacca caaatatgcg ccgcctaacg aagcccatac cagaccgagt gtcagtgacg
     6601 ttgactctag gttaacccac agcacaccca ccgtcagcgc gccaaccagc ggcatcagca
     6661 aatagtggaa gtgatccttc cagcttttat tcattccttt acgccgccag aaatgattaa
     6721 acaccgacag attcacgaag gtaaacgcca ccagagcacc gaagttaatc agtgctgtcg
     6781 cggtgactaa atcaaagaac agcgccgaca gcgcgacaat cccgaccata atgacgttga
     6841 gtgccggagt ccgccatttt gggtgcacat agccaaacac gcgctccgga aacacattgt
     6901 cacgccccat cacatacagc agacgcgaca cgctggcatg tgaagccagg ccagacgcta
     6961 acgtgttcac aaacgtggtg cagaggaaaa tcgactggaa gagcttgccg ccgacgtaca
     7021 gtgcaatttc aggcagtgcg gcgtccgggt ctttaaagcg gctgatatcg ggaaagaaca
     7081 gctgcataaa gaacgacgcc gcgataaaga taacgccgcc gtataccgcc gtcaggaaga
     7141 tggctttcgg gatcacgcgt gcggcgtccg gcgtctcttc cgaaagcgtg gtcaccgcat
     7201 cgaaaccgag gaacgagaaa cagacaatcg tcgcaccggt aataatcggg atcaggtgcg
     7261 cgttctcgct gataaacggc tgaagtgacc agacggtgcc aacgccttct cctttatgca
     7321 gtccctgaac caccaggaag ataaacacca ccatgatgga gatttgcacc agtacaaaca
     7381 gggtattgaa gttagcgacc aggttgacgc tcttcagatt cgcggcggtt aaaatggcga
     7441 cgaaggttac cacccacacc cacggcggca cttccgggaa gagggcggag agatagattt
     7501 tcgccaacaa aacgttgatc attggcaaaa agagataatc cagcagtgat gaccagccga
     7561 ccataaatcc gacgtgcgga ttaatcgact tttgcgcgta ggtataggcc gaaccggcct
     7621 ccggaaactg gcgaaccagt ttgccgtagc tgatagcggt aaacagcacg cccgccagcg
     7681 ccagcaaata ggacgccgga acgtgaccgt cgctaatgcc ggagacaata ccaaaagtat
     7741 caaatacggt catcggcgtg agataggcca gacccatcat taccacctgc cacaatttca
     7801 gtgattttcg cagacgggtt ttgccgggtt gcgcagcaat attcagtggt gaattaatag
     7861 ccataattgc tgcctcccct gcgtaataag gtttgcggtc gtgtcgacct gaaatcaaac
     7921 ctgtttagca agggaagcaa agggggcgga gtccagtcag tagcaggagg ggcgcatacg
     7981 cagcctccct cgccatgcgg ggatttaatc atcggcatca tctcatttcc tcgtcacagt
     8041 cttaatcgtt aacggaagcc cgttgccgcc tgcgctccat aacgcgacat ccgggaaatg
     8101 tctggattac cagcaaatgt caggcctggc tccgctcagg ccgatgaaac aaccccgcaa
     8161 ggggaattac gcgtttttca acatccactc aatttctgtt tctgtgatga ggcgctcaaa
     8221 ctgcaacagc tcatcatttt tacaggcgtg atacacatgg caaaaccgtt cgcctaaata
     8281 gcggcgcagg tgatcattct cgataaactc acccagggca tcgctctggc gaatcgggaa
     8341 gggtaagcct tcctgttcca gcccgttgcc ttcgacttct tcctgcaacg gcagctcgtt
     8401 atcaaggcca tgcaaaatac cggcaaaaat cgctgccatc accagatatg ggttggcatc
     8461 ggcacccgcc acgcgatatt ccacgcggtg attatgacgg tcgccgcacg gaatacgcag
     8521 ggcgacggtg cggttgttat ggccccacga cgcctgcgtc ggcacataca ttcccggctg
     8581 gaagcggcga tacgagttca cgtttggtgc cagcaacgcc attgatgacg gcatcaggtc
     8641 aatcatcccg gcgagcatct ttttcagcag cggcgaatct tcgccttctg tgtcagaaag
     8701 cacgttctcg ccacgattat tttgcatact gatatggata tgcattccgc tgcccgcgtg
     8761 ctcttcatac ggcttcgcca taaaagtggc gtgcatctta tgcttttctg ccatcagacg
     8821 caccaggcgt tttagtgcca gcgcatcatc gcaggcttcc agcacgttat cggtatggta
     8881 aaggttgatt tcaaactgac ccggcgaggc ttcggcgacc gcaccgtcgg cgggaattaa
     8941 ctgtaactgc gccagttcat caatatcatt gagcacgtcg gcgaagtggt tgaggttatc
     9001 aacggagtaa acctggcttt gtgtattgcg gtcatcggtg ccgggcgcgc agggcggttg
     9061 cagataccct tcagcgtcgc gctgacgatc cagtaaatag aactccagct ctaccgctac
     9121 aaccgggaac aatccgcgct ggcgtagctg ctgccacagg cggttaagaa cgttccgcgg
     9181 ctcaacgtca aagggagcgc catcttcatc gaccatggtc aggagcatct gaccgataaa
     9241 ctctggatcg gcggcagaag gggttaagga accgagaaca ggaacacaag tacgatccgg
     9301 ctcgcccatt tcctgaccca gacccgcttc ttctactacg ttgcccagaa tatccattgc
     9361 aaacaccgag gccgggaaat aacacccttt ctccaacttc ttcaggcttg aaacagggat
     9421 acgttttcca cggaagcaac cattcaaatc agtaagtaaa acatcaacat attgcgtatt
     9481 cgggtagcgc tccaggtagc gtttcacttc ctgcgtaaag gcgctacccc gcctctcttc
     9541 tgactgctga acaaagttct ctacttcaac gatattggtt tccatgattc ttcgcctttg
     9601 gtttgttttc cgctcgttat caaagcgtaa aatataatga ccaccattcg aatctgtatg
     9661 caaactaaat gtttgtcaaa tgttaaattt agtttgcaaa aatgaaaatc cactgctaga
     9721 ttgaaaaaat attgaacata aaggtcattt aaagcgcagt aatggcgata atttagtcca
     9781 ctttgtgaga ttgagcatgg aaaatataat gaacaatccg gttatcggtg tcgtaatgtg
     9841 caggaacagg cttaagggtc atgcgaccca gactctgcaa gaaaagtacc tgaatgccat
     9901 catccacgca ggcggcttgc caattgcgct accacatgcg ctggcggaac cgtcgttact
     9961 tgaacaactt ttgccgaaac tcgatggcat ttatcttcct ggtagtccca gcaatgtgca
    10021 gccgcaccta tatggtgaaa acggcgatga gcctgacgcc gatcctgggc gtgatcttct
    10081 gagcatggcg ataattaatg ccgcactcga aaggcgcatc cccattttcg ccatctgccg
    10141 gggtttacaa gaactggtgg tggcaaccgg tggttcgttg catcgcaagc tgtgcgaaca
    10201 gcctgaattg ctggaacatc gggaagatcc cgaactgccg gtggaacagc aatatgcacc
    10261 gtcgcatgaa gttcaggttg aagagggggg attactgtct gcgttgttac ctgaatgtag
    10321 caacttttgg gtaaactctc tacatggaca aggggcgaag gtcgttagcc cacggttgcg
    10381 tgttgaagct cgctcgccgg atggcttagt tgaggcggtt agcgtcatca atcatccttt
    10441 tgcgctgggc gtacagtggc acccggaatg gaacagtagc gagtacgcgc tttcgcgtat
    10501 attgttcgag ggctttatca ccgcttgtca gcaccatatc gctgaaaaac agcgactctg
    10561 accactacag tttaaggaaa tgcaaatatg agtgatgagg gactggcgcc aggaaaacgc
    10621 ttgtcggaaa tccgccagca gcaggggctt tcacaacgtc gtgccgccga actctccggg
    10681 ctgactcaca gtgctatcag tacgatagaa caagataaag tcagccctgc catcagtacg
    10741 ctgcaaaagc tgctgaaggt gtatggtctg tcactctcgg aattcttttc cgagccggaa
    10801 aaacctgatg agccgcaggt cgtcattaat caggacgact taattgagat gggcagtcag
    10861 ggtgtgtcaa tgaagctggt tcataacggt aacccgaatc gcacgctggc gatgatcttt
    10921 gaaacgtacc agccgggcac aaccactggg gaaagaatta agcatcaggg tgaggaaata
    10981 ggcacagtac tggaaggtga aattgttctg acgattaatg gtcaggatta ccacctcgtc
    11041 gcggggcaaa gttatgccat taataccggc atcccgcaca gtttcagtaa tacgtcggca
    11101 ggtatttgcc gaattatcag cgcccatacg cccaccacgt tttaatcttt ttgttctgta
    11161 agccgggtaa gcgcaatgcg cccggcaatc tatacacaaa atcattcaag ttgcatcaag
    11221 gcggcaagtg agcgaatccc gatgagctta ctcaggtaag tgattcgggg gagcgaacgc
    11281 agccaaggca gaggcggctt gaaggatgaa gtgtatataa agaatgtcgc cagaaataaa
    11341 cgggcatacg gcccggggat ctctgcgccc tgacgttcac aaactgcata tatctgatag
    11401 acgtgaaaca ggagtcataa tgaattttca tcatctggct tactggcagg ataaagcgtt
    11461 aagtctcgcc attgaaaacc gcttatttat taacggtgaa tatactgctg cggcggaaaa
    11521 tgaaaccttt gaaaccgttg atccggtcac ccaggcaccg ctggcgaaaa ttgcccgcgg
    11581 caagagcgtc gatatcgacc gtgcggtgag cgcagcacgc ggcgtatttg aacgcggcga
    11641 ctggtcactc tcttctccgg ctaaacgtaa agcggtactg aataaactcg ccgatttaat
    11701 ggaagccaac gccgaagagc tggcactgct ggaaactctc gacaccggca aaccgattcg
    11761 tcatagtctg cgtgatgata ttcccggcgc ggcgcgcgcc attcgctggt acgccgaagc
    11821 gatcgacaaa gtgtatggcg aagtggcgac caccagtagc catgagctgg cgatgatcgt
    11881 gcgtgaaccg gtcggcgtaa ttgccgccat cgtgccgtgg aacttcccgc tgctgctgac
    11941 ttgctggaaa ctcggcccgg cgctggcggc gggaaacagc gtgattctaa aaccgtctga
    12001 aaaatcaccg ctcagtgcga ttcgtctcgc cgggctggcg aaagaagcag gtctgccgga
    12061 tggcgtactg aacgtagtaa cgggttttgg tcatgaagcg gggcaggcgc tttcgcgtca
    12121 taacgatatc gatgccatcg cctttaccgg ttcaacccgt accgggaaac agctgctgaa
    12181 agatgcgggc gacagcaaca tgaaacgcgt ctggctggaa gcgggcggca aaagcgccaa
    12241 catcgttttc gctgactgtc cggatttgca acaggcggca agcgccaccg cagcaggcat
    12301 cttctacaac caggggcagg tgtgcatcgc cggaacgcgc ctgttgctgg aagagagcat
    12361 tgccgatgaa ttcttagccc tgttaaaaca gcaggcgcaa aactggcagc cggggcatcc
    12421 gctcgacccc gcaaccacca tgggcacctt aatcgactgc gcccacgccg actcggtcca
    12481 tagctttatt caggagggcg aaggtaaagg gcaactgctg ctggatggcc gcaacgccga
    12541 actggctgcg gccatcggcc cgacaatctt tgtggatgta gacccgaatg cgtccttaag
    12601 ccgcgaagag attttcggtc cggtgctggt ggtcacgcgt ttcacatcag aagaacaggc
    12661 gctacagctt gccaacgaca gccagtacgg ccttggcgcg gcggtatgga cgcgcgacct
    12721 ctcccgcgcg caccgcatga gccgacgcct gaaagccggt tccgtcttcg tcaataacta
    12781 caacgacggc gatatgaccg tgccgtttgg cggctataag cagagcggca acggtcgcga
    12841 caaatccctg catgcccttg aaaaattcac cgaactgaaa accatctgga taagcctgga
    12901 ggcctgaaat gaccgaacat accagcagtt attacgccgc cagtgcgcat aaatacgcgc
    12961 cattcgacac gctgaatgag tcaatcacct gcgacgtttg cgtggtcggc ggcggctata
    13021 ccgggctgtc ttccgcgcta catctggcgg aagcgggctt tgacgtagtg gttctcgaag
    13081 cctcacgcat cggctttggc gccagcggac gcaacggcgg acagcttgtg aactcctaca
    13141 gccgcgacat tgacgtgatc gaaaaaagct acggcatgga caccgcccgt atgctcggca
    13201 gcatgatgtt tgagggtggt gagatcatcc gcgaacgtat caaacgttat cagattgact
    13261 gcgattaccg ccccggcggt ctgtttgtgg cgatgaatga taaacagctc gccacacttg
    13321 aagagcagaa agagaactgg gaacgctacg gcaataaaca gctggagatg ctggacgcca
    13381 acgccattcg ccgcgaggtc gccagcgatc gctacactgg tgcgctgctg gatcacagcg
    13441 gcgggcatat tcacccgctg aacctcgcca ttggtgaagc ggacgctatc cgcctgaacg
    13501 gcgggcgcgt gtatgagctt tccgcagtga cgcagatcca gcacaccacg cccgccgtag
    13561 tgcgtacagc cacaggtcag gtgacggcga agtatgtgat tgtcgccggg aatgcctacc
    13621 tgggcgataa agtggagccg gagctggcga aacgcagcat gccgtgcggc acgcaggtga
    13681 tcaccaccga gcggctgtcg gaagatttag cccgttccct tatcccgaaa aactactgtg
    13741 tggaagactg taactatctg cttgattact atcgtcttac cgccgataac cgcctgctgt
    13801 atggcggcgg cgtagtgtac ggcgcgcgcg acccggatga cgttgagcgt cttgtggtgc
    13861 cgaaactgct gaaaaccttc ccgcagttga agggcgttaa aatcgactat cgctggacgg
    13921 gcaacttcct gctgaccctt tcacggatgc cgcagttcgg tcgcctcgat accaacatct
    13981 attacatgca gggctacagc ggccacggtg tgacctgtac ccatctcgcc gggcgtctga
    14041 ttgctgaact gctgcgcggc gacgccgagc gtttcgacgc cttcgccaat ctgccgcatt
    14101 atccgttccc cggcgggcgc acgctgcgtg tgccgtttac cgcgatgggc gcggcgtatt
    14161 acagcctgcg cgatcgtctg ggcgtttaat ttccgattaa cagtgaagag tcaaaaggtg
    14221 tgaaacatga gcaacaatga attccatcag cgtcgtcttt ctgccacccc gcgcggggtt
    14281 ggcgtgatgt gtaacttctt cgcccagtcg gctgaaaacg ccacgctgaa ggatgttgag
    14341 ggcaacgagt acatcgattt cgccgcaggc attgcggtgc tgaacacggg gcatcgccac
    14401 cctgaattgg tcgcggcggt ggagcagcaa ctgcaacagt ttacccacac cgcgtaccag
    14461 attgtgccgt atgaaagcta cgtcaccctg gcagagaaaa ttaacgcctt tgccccattg
    14521 agcgggcagg ctaaaaccgc gttcttcacc accggtgcgg aagcggtgga aaacgcggtg
    14581 aaaatcgccc gcgcccatac cggacgccct ggcgtgattg cgtttagcgg cggctttcac
    14641 ggtcgtacgt atatgactat ggcgttaact ggaaaggtcg cgccgtacaa aatcggcttc
    14701 ggcccgttcc ctggttcggt gtatcacgta ccttatccgt cagatttaca cggtatttca
    14761 acacaggact ccctcgacgc catcgaacgc ttgtttaaat cagacatcga agcgaagcag
    14821 gtcgcggcca ttattttcga accggtgcag ggcgagggcg gtttcaacgt tgcacccaaa
    14881 gagctggttg ccgccattcg ccgcctgtgc gacgagcacg gtattgtgat gattgctgat
    14941 gaagtgcaaa gcggctttgc gcgtaccggt aagctgtttg ccatggaaca ttacgccgat
    15001 aagccggatt taatgacgat ggcgaaaagc ctcgcgggcg ggatgccgct ttcgggcgtg
    15061 gtcggtaatg cgaatattat ggacgcgccc gcgccgggcg ggttgggtgg cacctacgcc
    15121 gggaacccgc tggcggtggc tgccgcgcac gcggtactca acattatcga caaagaatcg
    15181 ctctgcgaac gcgcgaatca actgggccag cgtctgacaa acacgttgat tgatgccaaa
    15241 gaaagcgttc cggccatcgc ggcggtacgc ggcctggggt ctatgatcgc ggcagagttt
    15301 aacgatccgc aaacgggcga gccgtcagcg gcgattgcac agaaaatcca gcaacgcgcg
    15361 ctggcgcagg ggctgctcct gctgacctgt ggcgcatacg gcaacgtgat tcgcttcctg
    15421 tatccgctga ccatcccgga tgcgcaattc gatgcggcaa tgaaaatttt gcaggatgcg
    15481 ctgagcgatt aatagcccaa cgcaataatg tctgatgcgc tgcgcttatc aggcctgcaa
    15541 acgacgttga ttatgtatgc cggataaagc attcacgccg catccggcaa gttgtattgc
    15601 ccaacttcgc taaatctggt gctttttcaa caacgcgcgg aactgatgat aggttaaccc
    15661 cagtaattca gccgcgcgct tctggttata tttcccttgt tgcaaactga gttgcagcaa
    15721 ctctttttcc tgctgcatct gaaactcacg taaatccagc ggcagtgttg gaagcgaggt
    15781 ggtttctgaa acggcgatag cgtcttcagg cggacgccgt ttaaagggat caataatgat
    15841 gtcatcaagc ggataatcgc tggtgccgtg gcgatacact gaacgttcca ccacgttttt
    15901 caattcacga atatttcccg gccaacgata attcagcaat gtttctctgg cgcgctccgt
    15961 aaaccccggg aacagaggca gcttgatttc ccgacacatc tggatggcaa agtgttctgc
    16021 catcaacatt atgtcgcttt cgcgctcgcg cagtggtggc agttgcacta catcgaaagc
    16081 cagccggtcg agcaggtctg cgcgaaaagt gccttcattg accatcgccg ggagatcggc
    16141 attcgtcgcg cataccaacc gcacattcac ctgcaacggc tggctgccgc caacgcgttc
    16201 cagttcaccg tactcaatca cgcgcaataa tttttcctgc accatcatcg gtgcagtagc
    16261 gagttcatca agaaatagcg taccgccgtc ggcacgttca aatctccctg gatgacgttt
    16321 ttgcgcaccg gtaaacgcgc ccgcttcgtg accaaacagt tcggaatcca gcagattttc
    16381 atttaacgcc gcgcagttaa gggaaataaa cggcccttgc caacgggagg agagataatg
    16441 caggcggctg gcgatcagct ctttaccggt gccgcgctcg ccgatgatga gcacaggttt
    16501 gtccagcggt gcgagatgcg aaacctgttc cagcacttcg agaaagctgt tcgcctcacc
    16561 aagtaaatta tctttgtatt ctgccatgat gaaattcgcc acttgttagt gtaattcgct
    16621 aactcatcct ggcatgttgc tgttgattct tcaatcagat ctttataaat caaaaagata
    16681 aaaaattggc acgcaaattg tattaacagt tcagcaggac aatcctgaac gcagaaatca
    16741 agaggacaac attatgggta ttttttctcg ctttgccgac atcgtgaatg ccaacatcaa
    16801 cgctctgtta gagaaagcgg aagatccaca gaaactggtt cgtctgatga tccaggagat
    16861 ggaagataca ctggttgaag tacgttctac ttcggcgcgc gcgttggcag aaaagaaaca
    16921 gctgactcgc cgtattgaac aagcgtcggc gcgtgaagtt gaatggcagg aaaaagccga
    16981 actggcgctg ctgaaagaga gagaggatct ggcacgtgca gcgttaattg aaaaacagaa
    17041 actgaccgat ctgattaagt ccctggaaca tgaagtgacg ctggtggacg atacgctggc
    17101 acgcatgaag aaagagattg gcgagctgga aaacaaattg agcgaaacac gcgctcgcca
    17161 gcaggcattg atgttacgtc atcaggcggc aaactcgtcg cgcgatgtgc gtcgtcagct
    17221 ggacagtggc aaactggatg aagcaatggc tcgtttcgaa tctttcgaac gtcgtattga
    17281 ccagatggaa gcggaagcag aaagccacag cttcggtaaa caaaaatcgc tggacgatca
    17341 gtttgccgaa ctgaaagccg atgatgcaat aagcgagcaa ctggcacaat taaaagccaa
    17401 aatgaagcaa gacaatcaat aataacatcc aggcggcgtc cgaaagcgcc gccgctcatc
    17461 gtctaaggag tacttatgag cgcgctattt ctggctattc cattaaccat ttttgtgctg
    17521 tttgttttac cgatctggtt atggctgcat tacagcaatc gttctggtcg cagtgaattg
    17581 tcgcaaagtg agcagcagcg attagcgcaa ctggctgatg aagcaaaacg gatgcgcgaa
    17641 cgtattcagg cgctggaatc tattcttgat gcagaacatc cgaactggag ggatcgctaa
    17701 tggcgggcat taatctcaat aaaaaattat ggcgtattcc acagcagggc atggtccgtg
    17761 gcgtctgcgc cgggattgcc aactattttg atataccggt aaaactggtg cgtatcctgg
    17821 tggtgctgtc gattttcttc ggtctggcgc tgtttaccct ggttgcttac atcattttgt
    17881 catttgcgct tgatccaatg ccggacaaca tggcctttgg tgagcagcta ccttctagca
    17941 gtgaattgct ggatgaagtc gaccgtgaac tggcggcaag tgaaacgcgt ttacgcgaga
    18001 tggaacgtta tgtcacttcc gatacattca cgttacgtag ccgtttccgc caactgtgag
    18061 gaaagttatg aatactcgct ggcaacaggc cgggcaaaag gtaaagcctg gtttcaaatt
    18121 agcaggcaag ctggttcttc ttaccgcact gcgctatggc ccagcgggtg tggcgggctg
    18181 ggcgataaaa tcagttgctc gccgaccgct gaaaatgttg ctggctgtgg cgctggaacc
    18241 gctgttaagt cgggctgcta ataaactggc acagcgttat aaaaggtgag gggagtttcg
    18301 caaaaattgt taaatctcag gcctataatg gatggcaatt ttcatccata gaaggacgct
    18361 tacatgttta aaaaaggctt acttgctctg gcactggtgt tttcactgcc cgtttttgcc
    18421 gctgaacact ggatcgatgt tcgtgttcca gagcagtatc agcaagagca cgttcagggg
    18481 gccatcaata ttcccctgaa ggaagtgaaa gagcgcattg ccaccgccgt tccggataaa
    18541 aacgacaccg tgaaagtgta ttgcaatgcc ggacgccagt cagggcaggc aaaagagatc
    18601 cttagcgaga tgggatatac ccacgttgag aacgccggtg gcctgaaaga catcgcaatg
    18661 ccgaaggtca aaggttaaaa gaattcacca tgagcggcgc ttatgcgccg tttttttctg
    18721 tcacacctta tttacatccc catagattat ttgcgtcagc tcacaaatac gctttttccc
    18781 tggtaaaaaa tgatttcctg cgtgactaaa acccttgtgc tcaattgaca gtttattttc
    18841 tgcggagtag tctctcgttt catgggaccg ctaccacgga aaggcgacat gaaacagaaa
    18901 attacggatt acctggacga aatctacggt ggaacattta ccgcaactca tttacagaaa
    18961 cttgtaacgc gtcttgagag tgcgaaacga ttaattacac agcgacgtaa aaaacactgg
    19021 gatgaaagtg atgtcgtgtt aatcacctat gccgatcaat ttcacagcaa tgatttaaaa
    19081 ccattaccca catttaatca gttttatcat caatggctgc aaagcatttt ttcacatgtt
    19141 catttgttgc cgttttatcc atggtcatct gatgatggct tttcggtaat tgattatcat
    19201 caggtcgcca gtgaagcggg ggagtggcag gatattcagc aactcggtga atgcagtcat
    19261 ttaatgtttg attttgtctg caaccatatg tcggcaaaaa gtgaatggtt taaaaactat
    19321 ttacaacagc atccaggttt tgaagatttt tttattgccg ttgacccgca aaccgatctc
    19381 agcgccgtca ctcgcccgcg tgcgttaccg ttattaacgc cattccagat gcgcgatcat
    19441 tcaacgcgcc atttatggac cacctttagt gacgatcaaa ttgacctgaa ttaccgtagc
    19501 cctgaagtgt tgctggcgat ggtggatgtt ttactctgtt accttgcgaa aggtgctgag
    19561 tatgtccgcc tggatgccgt tggctttatg tggaaagagc cgggaacaag ctgcatccat
    19621 ctggaaaaaa cacatctgat tatcaagctg ttacggtcga ttattaatga cgtagcacca
    19681 ggtacagtga tcattaccga aaccaatgtt ccgcacaaag acaacattgc ttactttggc
    19741 aacggtgatg acgaagcgca tatggtgtac cagttctcgc tgccgccgct ggtgttacat
    19801 gcggtgcaaa aacagaacgt cgaggcgctg tgccagtggg cgcaaagtct gagcttgccc
    19861 tcagataaca ccacctggtt taacttcctc gcctctcacg atggcatcgg gctaaacccg
    19921 ctacggggct tgttgcctga aagcgaaata ttagagctgg tcgaggcgtt acagcaggaa
    19981 ggtgcattag taaactggaa aaataatccc gacggtacac gcagtccgta tgaaataaat
    20041 gtgacctata tggatgcgtt aagccgccgt gagagtagcg atgaagaacg ttgcgccagg
    20101 tttattctcg cccatgcgat tttgttaagt ttccctggtg tgccagcgat atatattcaa
    20161 agtattcttg gctcgcgtaa tgattacgca ggtgtcgaaa aactcggata taaccgtgcg
    20221 attaaccgta aaaaatatca cagtaaacag ataacccgag aactgaacga tgaagctaca
    20281 ttaaggcatg cggtatatca tgagttgtcg cgtttaatta cacttcgtcg cagccataac
    20341 gagttttatc cggataataa ttttaccatc gatacgatta attcatccgt aatgcgtatt
    20401 caaagaagta acgctgatgg taattgtctg actggattgt ttaatgtcag taaaaatatt
    20461 cagcatgtaa atattactaa tctgcatggt cgggatctga ttagtgaagt tgatatattg
    20521 ggtaatgaaa taacgctgcg cccctggcag gttatgtgga ttaaataaaa aggaacatct
    20581 catgattaaa tcaaaaatcg tgctgttatc agcactggtt tcctgcgccc tgatttcagg
    20641 ctgtaaagaa gaaaataaaa cccatgtatc catcgaattt atgcattctt cggtggagca
    20701 ggagcgtcag gcagttatca gcaaattgat tgcccgtttt gaaaaagaaa acccaggtat
    20761 caccgttaaa caagtgcccg tcgaagagga cgcttataac accaaagtca tcaccctttc
    20821 acgtagcggc tcgctgccgg aagtgatcga aaccagccat gactacgcca aagttatgga
    20881 taaagagcag cttatcgatc gcaaggcggt tgccacggtc atcagcaacg ttggtgaagg
    20941 cgcgttttac gatggcgtac tgcgtattgt gcgtaccgaa gatggtagcg catggaccgg
    21001 tgttcctgtc agcgcctgga ttggcggcat ctggtatcgc aaagatgtgc tggcaaaagc
    21061 ggggcttgag gagccgaaaa actggcaaca gctgctggac gttgcacaga aactgaatga
    21121 cccggcgaat aaaaaatacg gcattgcgct gcctacagca gaaagcgtgt tgacggaaca
    21181 atccttctcc cagtttgcgt tatccaacca ggctaacgtc tttaacgccg aaggcaaaat
    21241 cacccttgat acaccagaga tgatgcaggc gctgacctat taccgcgacc ttgctgccaa
    21301 caccatgccg ggttctaacg acatcatgga agtgaaagac gcctttatga acggcaccgc
    21361 gccgatggcg atttactcca cctatatcct tccggctgtg attaaagaag gcgacccgaa
    21421 aaacgtcggt ttcgtggtgc caaccgagaa aaactctgcg gtctacggca tgttgacctc
    21481 gctgaccatt accgcagggc aaaagaccga agagacggaa gcagctgaaa aatttgtcac
    21541 ctttatggag caggcagaca acattgccga ctgggtgatg atgtcgccag gtgctgcgct
    21601 gccggtgaat aaagcggttg tcactaccgc cacctggaaa gacaacgacg ttattaaggc
    21661 gctgggtgaa ctaccgaatc agctaatcgg tgaactgcca aatattcagg tctttggcgc
    21721 agtaggggat aaaaacttta cccgcatggg tgatgtgacg ggttctggcg tggtgagctc
    21781 catggtgcat aacgtcaccg tgggtaaagc cgatctccct ggcacgctgc aagcgagcca
    21841 gaaaaagctg gatgaactga tcgaacagca ctaaacccag gacaggaatc cgcaatgaac
    21901 aggctttttt caggtcgttc cgatatgccc tttgcgctgc tgcttctcgc gcccagctta
    21961 ttactgctgg gtggtctggt ggcctggcca atggtgtcga atatcgaaat cagtttttta
    22021 cgtctgccgc tcaatcccaa catcgagtca acctttgttg gggtgagtaa ctatgtgcgt
    22081 atcctctccg atcccggctt ctggcactcg ctgtggatga cggtctggta taccgcgctg
    22141 gtggtggcgg gcagcaccgt gctcgggctg gcagtggcga tgtttttcaa ccgcgagttc
    22201 cgcctgcgca aaaccgcgcg ttcgctggtg atcctctcct acgtaacgcc gtccatctcg
    22261 ctggtgtttg cctggaaata catgttcaac aacggctacg gcattgtgaa ctacctcggc
    22321 gtcgatcttc tgcatctcta tgagcaggca ccgctgtggt tcgataatcc gggcagtagc
    22381 tttgtgctgg tggtgctgtt cgccatctgg cgctacttcc cgtatgcctt tatctcgttt
    22441 ctggcgattt tgcagaccat cgacaaatcg ctgtacgaag cggcagagat ggatggcgct
    22501 aatgcctggc aacggtttcg tatcgtcacg ctgcccgcga ttatgccggt cctggcgacg
    22561 gtggtgaccc tgcgcaccat ctggatgttc tacatgttcg cggatgttta tttgctgacg
    22621 accaaggtcg atattctcgg tgtatatctc tacaaaaccg cctttgcctt taatgattta
    22681 ggaaaagcgg cggcgatctc ggtggtgctc ttcatcatta ttttcgctgt cattctgctg
    22741 accaggaaac gggtgaacct caatggcaac aaataaacgc acactcagtc gcatcggttt
    22801 ttactgcggg ctggcgctgt ttctcatcat cacgctgttt ccattttttg tgatgctgat
    22861 gacctcgttc aagggcgcga aagaggcgat ctcactgcat cctacgctgc tgccgcagca
    22921 gtggacgctg gagcattacg tcgacatttt taacccgatg attttcccgt ttgtcgacta
    22981 cttccgtaac agtctggtgg tgtctgtggt ttcatccgtg gtggcggtat tcctcggcat
    23041 tctcggagct tacgcgcttt ctcgcctgcg ctttaaaggc aggatgacca tcaacgccag
    23101 cttttacacg gtgtatatgt tctccggcat tttgctggtg gtgccactgt tcaaaatcat
    23161 caccgcgctt ggtatttacg acaccgagat ggcgctgatc atcaccatgg tgacgcaaac
    23221 cctgcccacc gccgtgttta tgctgaaaag ctacttcgac accatccctg atgagatcga
    23281 agaagcggcg atgatggatg gcctcaaccg tctgcaaatt atcttccgta tcaccgtgcc
    23341 cctggcgatg tccgggctga tatcggtgtt cgtctactgc tttatggtgg cgtggaacga
    23401 ctatctgttt gcgtcgattt tcctctccag cgccagcaat ttcaccttac cggtgggcct
    23461 gaacgcgctg ttcagtacgc cagattacat ctggggacgg atgatggcgg cctcactggt
    23521 gaccgcattg ccggtggtga ttatgtatgc gctttcggaa cgttttatta agagtggttt
    23581 gaccgccggt ggcgtgaagg gctaaagcgg ccagtttctt aacaaggagt tttacatgaa
    23641 aaagttagta gccacagcac cgcgcgttgc tgcgctggtt gagtatgaag atcgggcgat
    23701 tttagccaat gaagtgaaga tccgcgtgcg tttcggcgca ccgaaacacg gaacggaagt
    23761 ggtcgacttc cgcgccgcca gcccgtttat cgatgaagac tttaacggcg agtggcagat
    23821 gttcaccccg cgtccggcag atgcgccgcg cggcattgag tttggcaaat tccagcttgg
    23881 caacatggtg gttggcgaca ttatcgagtg cggcagcgac gttacagact acgcggtggg
    23941 cgacagcgta tgcggctacg gcccgctctc cgagacggtc atcattaacg cggtgaataa
    24001 ctacaagctg cgcaaaatgc cggaaggcag ctcctggaaa aacgccgttt gttacgaccc
    24061 ggcgcagttt gccatgagcg gcgtgcgcga tgccaacgtg cgcgtgggtg attttgttgt
    24121 ggtggtaggg cttggcgcga tcggtcaaat tgccatccaa ctggcgaaac gcgctggcgc
    24181 atcggtggta attggcgtcg atcctatcgc ccatcgctgt gaaattgccc gtcgccacgg
    24241 tgctgatttc tgccttaacc ccattggcac tgacgtaggc aaagagatca aaacgctgac
    24301 cggcaagcag ggtgccgatg tgattatcga aaccagcggt tacgccgacg cgctgcaatc
    24361 ggcgctccgc ggtctggctt acggcggcac catctcctat gtcgcgtttg ccaaaccatt
    24421 tgctgaaggt tttaacctcg gacgcgaagc gcatttcaat aacgccaaaa ttgttttctc
    24481 ccgcgcgtgc agcgaaccga acccggatta tccgcgctgg agccgtaagc gtattgaaga
    24541 aacctgctgg aaactgctga tgaacggtta tctcaattgc gaagatttaa tcgacccggt
    24601 agtgaccttt gccaagagcc cggaaagcta catgcagtat gtcgatcagc atccggaaca
    24661 gagcatcaaa atgggcgtta cgttttaatt caaaggaatg aaaaaatgaa aataggcaca
    24721 cagaatcagg cattctttcc ggaaaatatc cgcgagaaat ttcgttatat caaagagatg
    24781 ggcttcgatg gttttgagat tgacggcaaa ctgctggtca acaacatcga agaagtcaaa
    24841 gcggcgatta aagaaaccgg tttaccggtg accaccgcct gcggcggcta tgacggctgg
    24901 attggtgact ttatcgaaga gcgtcgtctt aatggcttaa agcagatcga acgcattctc
    24961 gaagcgctgg cagaagtggg gggtaaaggt atcgtcgttc cggcagcgtg gggcatgttt
    25021 accttccgct taccgccgat gacctcgccg cgtagcctgg acggcgaccg taaaatggtg
    25081 agtgattccc tacgcgtact ggaacaggtc gccgcgcgta ccggaaccgt ggtgtatctc
    25141 gaaccgttaa accgttatca ggatcacatg atcaacaccc tcgccgatgc ccgccgttat
    25201 atcgtcgaaa acgatcttaa acatgtgcag attatcggcg atttctatca catgaatatt
    25261 gaagaagata acctggcgca ggcgctgcac gacaaccgtg acttgctcgg tcatgtgcat
    25321 attgccgata accatcgtta ccagccgggc agcggcaccc tcgatttcca cgcgctgttt
    25381 gaacagctgc gcgccgataa ctatcagggt tatgtggtgt atgaagggcg tatccgggca
    25441 gaagatcctg cccaggcgta ccgtgattcg ttggcctggt tgcgtacctg ctaagaggtc
    25501 tttgtgaaaa gtgcaatgac aagctctccg ctgcgtgtcg cgataatagg cgcaggccag
    25561 gtggcggata aggttcatgc ttcgtactac tgcacccgca acgatctgga actggtggct
    25621 gtctgtgaca gccgcctttc ccaggcgcag gcgctggcag aaaaatacgg gaatgcatcc
    25681 gtgtgggacg atccgcaggc catgctgctg gcggtgaaac ctgatgtggt tagcgtctgc
    25741 tcacctaacc gttttcatta cgaacatacc ctgatggcac tggaagcggg ctgccatgtg
    25801 atgtgcgaaa aaccgcccgc catgacgcca gaacaggcgc gggaaatgtg cgataccgcg
    25861 cgcaaactgg gcaaggtgct ggcctacgac tttcaccatc gttttgcgct cgatacgcaa
    25921 cagctgcgtg aacaggtgac caacggcgtt ttgggagaga tttacgttac caccgcccgc
    25981 gccctgcgtc gctgcggcgt tcccggctgg ggcgtcttta ccaataaaga actgcagggt
    26041 ggtggcccgc tgatcgacat cggcattcat atgctggatg ctgcgatgta tgtgctgggc
    26101 tttcctgcgg tgaaaagcgt gaatgcgcat agctttcaaa agatcggcac gcaaaagagc
    26161 tgtggtcaat ttggcgagtg ggatccggca acttacagcg tcgaagattc gctgtttggc
    26221 accattgaat ttcataacgg cggcattctg tggctggaaa cgtcatttgc gctcaacatc
    26281 cgcgaacagt cgattatgaa cgtcagtttt tgtggtgata aagccggtgc gacgctgttt
    26341 ccagcacata tctacaccga taacaacggt gaattaatga cgctgatgca acgggaaatg
    26401 gcagacgaca accgccattt gcgcagcatg gaagccttta tcaatcacgt acagggcaag
    26461 cccgtgatga tagccgatgc cgagcagggg tacatcatcc agcaactggt ggcggcgttg
    26521 tatcaatccg cagaaacagg gacgcgtgtg gaattatgac caggccagta acgttatcag
    26581 aaccccattt cagccagcat accctgaaca agtatgcatc actgatggcg caggggaacg
    26641 gctatcttgg gcttcgcgcc agccatgaag aagattacac gcgccagacg cgcggcatgt
    26701 atctggcggg gctgtatcat cgggcgggta aaggtgaaat caacgaactg gtgaacctgc
    26761 ccgatgtggt ggggatggag attgccataa atggtgaggt tttctcatta tcccgcgaag
    26821 cctggcagcg tgagcttgac tttgccagtg gcgaattacg ccgcagcgtt gtctggcgta
    26881 ccagcaacgt cacaggttac accatcgcca gccgtcgctt tgtttcggca gaccaactgc
    26941 cgctcattgc gctggaaatc actattactc cactggatgc cgacgcgtca gtgctgattt
    27001 caacaggcat tgacgccacg caaaccaacc acggtcgcca gcatctcgac gaaacccagg
    27061 tgcgggtgtt tggtcagcat ctgatgcagg ggatctacac cacccaggat ggacgcagtg
    27121 atgtggccat cagctgttgc tgtaaggtga gcggtgatgt gcagcaatgc tataccgcta
    27181 aagagcgccg tttgctgcaa cataccagtg cgcagcttca tgcaggcgag acagtgacgt
    27241 tgcaaaaact ggtgtggatc gactggcggg atgacaggca agccgtttta gacgagtggg
    27301 gcagcgcgtc gcttcgccag cttgaaatgt gcgcgcagca gagttacgac caacttcttg
    27361 cagcatcaac agaaaactgg cgtcaatggt ggcagaaacg tcgtatcacg gtaaatggcg
    27421 gcgatgcgca cgatcagcaa gcgttagatt atgcgcttta tcatctgcgc atcatgacgc
    27481 cggctcacga cgagcgcagc agtattgcgg caaaaggctt aaccggcgaa ggctacaaag
    27541 gccacgtttt ctgggataca gaagtatttt tgctgccgtt ccatctgttt agcgatccga
    27601 cggttgcccg aagtttactg cgttatcgct ggcacaactt gccaggcgcg caggagaaag
    27661 cacggcgcag cggctggcag ggcgcgctat ttccgtggga aagcgcgcgc agcggcgaag
    27721 aagagacgcc agaatttgcc gccattaaca ttcgtaccgg gctgcagcaa aaagtggcct
    27781 cggcgcaggc ggaacatcat ctggtggccg atatcgcctg ggcggttatt caatactggc
    27841 agaccacggg ggatgaaagt ttcattgctc atgaaggcat ggcgctactt ctggaaactg
    27901 caaagttctg gattagccgc gcggtgaggg ttaacgaccg tctggaaatt catgatgtta
    27961 ttgggccaga cgaatatacc gaacatgtca ataataacgc cttcaccagc tatatggcgt
    28021 attacaacgt ccagcaggcg ctgagtattg cccgccagtt tggctgtagc gacgacgcgt
    28081 ttattcatcg cgccgaaatg ttccttaaag aactgcggct gccagaaatt cagcccgacg
    28141 gcgttttgcc gcaggatgat tcgtttatgg ctaagccggc gattaatctg gcgaaataca
    28201 aagcggcggc ggggaagcaa accattctgc tggattattc acgcgcagaa gtgaacgaga
    28261 tgcaaatcct caaacaagct gatgtggtga tgctcaatta catgctgccg gagcagttct
    28321 cagcggcatc gtgtcttgcc aatctgcaat tttatgaacc gcgcactatt cacgactcgt
    28381 cattaagtaa agcaatccac ggcattgttg ccgcacgctg tggcctgctg acccaaagtt
    28441 atcagttctg gcgcgagggg actgaaatcg atcttggtgc tgatccgcat agttgtgatg
    28501 atggtatcca tgctgccgca actggcgcta tctggctggg ggcgattcag ggttttgccg
    28561 gggtgagcgt gcgtgacggt gaattgcatc tcaatccggc gttacctgag cagtggcaac
    28621 agttgtcttt ccctctgttc tggcagggct gcgaattaca ggtcactctt gacgcgcagc
    28681 gtattgcgat tcgaacttct gcgcccgttt cactgcgttt gaacggtcag cttatatccg
    28741 tggctgaaga atctgttttc tgtttgggtg attttatttt gcccttcaat gggaccgcta
    28801 ccacacatca ggaggatgaa tgaaactgca aggggtaatt ttcgatctgg atggtgtaat
    28861 caccgatacc gcgcatctgc atttccaggc gtggcagcag attgccgctg aaattggcat
    28921 cagcattgat gcgcagttta acgaatccct aaaagggatc agccgcgatg agtctctgcg
    28981 gcgcattctg caacacgggg gcaaagaggg cgactttaac tcgcaggaga gggcgcaact
    29041 ggcgtatcgc aaaaatctgc tctatgtcca ctcactgcgc gagttgacgg tcaacgctgt
    29101 tctacccggc attcgctctt tgctggcaga tctccgtgca cagcagatcc cggttgggct
    29161 ggcttctgtc tccctgaatg cgccgacgat tttagcggcg ctggagctgc gcgagttttt
    29221 caccttctgc gcggatgctt cccaacttaa aaactcgaaa ccggacccgg aaatctttct
    29281 cgccgcctgt gccgggctgg gcgtgccgcc gcaggcatgt atcggcattg aagatgcgca
    29341 ggcgggcatt gacgccatta acgccagcgg tatgcgctcg gtggggatcg gcgcgggctt
    29401 aaccgaggcg caattactgt tgccttcaac ggaatcactc acctggccgc ggttatcggc
    29461 cttctggcaa aacgtatagc aaaggaatca acatggctca gctttcgtta caacatattc
    29521 aaaaaatcta cgataaccag gtgcatgtgg tgaaggactt caacctggaa attgccgata
    29581 aagagttcat cgtgtttgtc ggcccgtcgg gctgcggtaa gtcgaccacc ctgcgcatga
    29641 ttgccgggct tgaggagatc agcggcggct atctgttgat cgacggcaaa cgaatgaatg
    29701 acgttccagc caaagcacgc aatatagcga tggtgttcca gaactacgcg ttgtatccgc
    29761 atatgacggt ttacgacaac atggcgtttg gcctgaagat gcaaaaaatc gccaaagagg
    29821 tgattgatga gcgggtgaac tgggcggcgc aaattctcgg cctgcgtgag tacctgaaac
    29881 gcaagccggg cgcgctttcc ggcgggcaac gccagcgtgt ggcgctcggg cgggcgattg
    29941 tgcgcgaagc gggcgtgttt ttaatggatg aaccgctctc taaccttgat gccaagctgc
    30001 gcgtgcaaat gcgcgcagag atcagcaagc tgcaccagaa gctgaatacc accatgatct
    30061 acgtgaccca cgatcagacc gaagcgatga ccatggcgac gcggattgtg attatgaagg
    30121 atgggattgt tcagcaagtt ggtgcgccga aaacggttta taaccaacca gcgaatatgt
    30181 ttgttgccgg atttattgga tcaccggcga tgaattttat tcgtggcacg atcgatggcg
    30241 ataaattcgt tacggaaacg cttaaattaa ccattcccga agagaaatta gcggttctga
    30301 aaacacaggg aagtttgcat aagcccatcg tgatgggaat acgtccggaa gatattcatc
    30361 cggacgcgca agaggaaaat aacatttccg ccaaaattag cgtggcagaa ttaaccggtg
    30421 cggaatttat gctctacacc acggttgggg ggcacgagtt agtggtccgt gctggtgcgt
    30481 taaatgatta tcatgcagga gaaaatatca ctattcattt tgatatgacg aaatgtcatt
    30541 tctttgatgc agaaacggaa atagcaattc gctaaataca gggggaaggc attcccccag
    30601 aataatacaa ggaacaataa tgaaaaagtt attaccctgt accgcactgg tgatgtgtgc
    30661 gggaatggcc tgcgcacagg ccgaggaaaa gaacgactgg cactttaata tcggcgcgat
    30721 gtacgaaata gaaaacgtcg agggttatgg cgaagatatg gatgggctgg cggagccttc
    30781 agtctatttt aatgccgcca acgggccgtg gagaattgct ctggcctatt atcaggaagg
    30841 gccggtagat tatagcgcgg gtaaacgtgg aacgtggttt gatcgcccgg agctggaggt
    30901 gcattatcag ttcctcgaaa acgatgattt cagtttcggc ctgaccggcg gtttccgtaa
    30961 ttatggttac cactacgttg atgagccggg taaagacacg gcaaatatgc agcgttggaa
    31021 agtcgcgcca gactgggatg taaaactgac tgacgattta cgcttcaacg gctggctggc
    31081 gatgtataag tttgccaacg atctgaatac gactggctat gctgataccc gtgtcgaaac
    31141 ggaaacgggc ctgcaatata ccattaatga aaccgtcggc gttcgggtga actattacct
    31201 ggagcgcggc tttaatatgg acgatagccg caacaacggt gagttttcaa cccaggagat
    31261 ccgtgcctat ttgcctataa cgctaggtaa tcattcgata acgccgtata cgcgaatcgg
    31321 actggatcgc tggaccaact gggactggca ggatgatatt gagcgtgaag gtcatgactt
    31381 taaccgtctt ggcttatttt acggctatga tttccagaac ggcctttccg tttcgctgga
    31441 gtacgcgttt gagtgggaag atcatgacga aggcgacagc gataaattcc attatgcagg
    31501 tgtcggcgta aattactcgt tctgataatg ggctaaattg ccggatgcgg cgcgagtact
    31561 ttatccgatc tataaatgta ggccggataa gatgcgccag catcgcatcc ggcattcagg
    31621 caaggtcgct ggtatttatt tcagcgtcat atgcgtggca acggtaatat tttgtggtga
    31681 cggttttccg gcaattaagc ggaataataa ctcacagctt tgttgaccta actcctgcgt
    31741 cggaacatcg atgccgcccg gtgcaggcgt taaaataaat gacagcgttt cattgctata
    31801 acccaccacc gctaactgct gcggaatagc aatgtttttc tctgccgccg cacgataaat
    31861 gctcattaat ttcaggctgt cagtggcaaa cactgcctca ggcaacggtg actgacctaa
    31921 taattgccgt gctgcttgca gtgctgtttc atgggtataa ccgccatcaa caatccattc
    31981 atcacgcact gcaatattat gatcttccag actctgctta taaccattta cccgatccac
    32041 tgaaacatgg acatcaagcg gtgcatgaag gcatgcaatg tttttatgtc cattctcaat
    32101 taatgcatcg gttagcgcaa tgctgtcacc aaaattatcg gtatcgacag aataaacatg
    32161 ggcatattga ccttcaactt tgccaatcac caccacagga atatcgtatt tatcaagttg
    32221 ggcaaaaaat gactcatccg ctggcgaact tagcataata atgcctttaa tcattttctg
    32281 cttaattttg ctttcacatt tttgtaagtc ttctgccggg gtgtgcgacg tctgcaatat
    32341 cacgtcgaaa ccttcttctt cagctttggc ggtgatggca tgcaaaactt cagagaaaaa
    32401 cggattaccc gccgtagttt tggtcgaacg ggtagaaatc accataatgg catcaaaacc
    32461 cgaagaggtc agcgcgcggg ccagcttgtt tggttgatac tgtaattctt caatggcccg
    32521 taacactttt tcgcgcgctt ccggggagat attagtttgc ttattcagca cgcgtgatac
    32581 ggtggatttt gatacgcctg caaccctggc aatatcataa atagtagggg acataggtca
    32641 ggaactccgt ccgaacgtga atgctgcaca tcttatggag ttcgcccgtc gatgacaaca
    32701 cgggtagttg ataatcaatg gcctggcccc cacattcata ttcttatgaa tgattttttt
    32761 tctggtcttc agagcagcac aggacagcaa tgaagcgact taaaaatgaa cttaatgcgc
    32821 tggtgaatcg gggtgtcgac agacatctgc gcctcgccgt aaccggcctt agccgcagcg
    32881 gcaaaacggc gtttatcact gcgatggtca atcagttgct caatattcac gccggagcac
    32941 gcttgccgct gttaagcgcg gtgcgtgaag aacggctgct gggcgtaaaa cgcattcccc
    33001 agcgtgactt tggcattccg cgcttcacct atgatgaagg gctggcgcag ttatatggcg
    33061 atccacctgc ctggccaacg ccaacgcgcg gcgtcagcga aattcgcctg gcgctacgtt
    33121 ttaaatcgaa cgattcgctg ctacgccact ttaaggatac ctccacgctg tatctggaga
    33181 ttgtggatta tcccggcgaa tggttgctcg acctgccgat gctggcgcag gactatttaa
    33241 gctggtcgcg ccagatgacg ggcttactta agggtcagcg cggcgaatgg tcggcgaaat
    33301 ggcgaatgat gtgcgaaggg ctggacccgc tagcgcctgc cgacgaaaac cggctggcag
    33361 acattgccgc cgcgtggacc gattatctcc accactgtaa acagcagggg ctgcacttta
    33421 ttcagccagg gcgctttgtc ttgccgggag atatggcagg tgcgcccgcg ctgcaattct
    33481 tcccgtggcc ggatgtcgat gcctggggcg agtccaaact cgcgcaggcc gataagcata
    33541 ccaatgccgg aatgctgcgc gagcggttta attattactg cgagaaagtg gtgaaggggt
    33601 tctataagaa tcattttctg cgctttgacc gccagattgt gctggtggat tgccttcaac
    33661 ctctcaacag tgggccacag gcatttaatg atatgcgtct ggcgctgacg cagctgatgc
    33721 aaagtttcca ctacgggcag cgtactctgt tcaggcgttt gttttcgccg gttatcgata
    33781 agctattgtt tgctgccact aaagcggacc atgtgaccat cgatcagcac gccaatatgg
    33841 tttcattgct gcaacaactg attcaggatg cctggcaaaa tgcggcgttc gaagggatca
    33901 gcatggactg cctggggctg gcgtcagttc aggcgaccac cagcggcatt attgatgtta
    33961 acggtgagaa aatcccggcg ctgcgcggta atcgacttag cgatggcgca ccgctcactg
    34021 tttatcctgg cgaagttccc gcacgtttgc ctggtcaggc gttctgggac aagcaagggt
    34081 tccagtttga agcgtttcgc ccgcaggtga tggatgtcga caaaccgctg ccgcatattc
    34141 gtcttgatgc cgcgctggaa tttttaatag gagataaatt gcgatgaccg aaccgttaaa
    34201 accacgtatt gatttcgacg gtccgctgga ggtcgatcag aatccaaaat tcagggcgca
    34261 gcagaccttt gacgaaactc aggcgcaaaa ttttgccccg gccacgctcg acgaagcgca
    34321 ggaagaagag gggcaagtcg aagcggtaat tgacgcagcg ttacgtccga aacgcagcct
    34381 gtggcgcaaa atggtgatgg gcgggctggc tctgtttggc gcaagcgttg tcgggcaggg
    34441 tgtacagtgg acaatgaatg cctggcaaac tcaggactgg gtggcgctgg gtggatgtgc
    34501 cgctggggca ttgattatcg gcgctggcgt aggttctgtg gtaacagagt ggcggcgctt
    34561 atggcgcttg cgacagcgcg cccatgaacg cgacgaagcg cgcgatttgt tgcacagcca
    34621 cggcacgggc aaaggccgcg cattttgcga aaaactggcg cagcaggcgg gtattgatca
    34681 gtctcatcca gcgctgcaac gctggtatgc ctcaatccat gaaacgcaga acgatcgtga
    34741 agtggtcagc ctgtatgctc atctggtcca gccggtttta gatgcccagg cgcggcgcga
    34801 aatcagccgc tcagcagctg aatcaacgtt gatgattgcg gtcagcccgc tggcgctggt
    34861 ggatatggca tttatcgcct ggcgcaatct gcgtttgatt aatcgcatcg ccacgctgta
    34921 tggcattgaa ctgggatatt acagccgttt gcgcctgttc aagctggtat tgctgaatat
    34981 cgctttcgcc ggagccagcg aactggtgcg cgaagtgggg atggactgga tgtcgcaaga
    35041 tctcgctgct cgtttgtcta cccgcgcagc tcaggggatt ggtgctgggc ttctgacggc
    35101 acgactcggg attaaagcta tggagctttg ccgcccactg ccgtggattg acgatgacaa
    35161 acctcgcctt ggggatttcc gtcgtcagct tatcggtcag gtgaaagaaa cgctgcaaaa
    35221 aggcaaaacg cccagcgaaa aataatgcaa tatcgggtgc tgaccggata tctttacgcc
    35281 gaagtgcccg tttttccgtc tttgtgtcaa tgattgttga cagaaacctt cctgctatcc
    35341 aaatagtgtc atatcatcat attaattgtt cttttttcag gtgaaggttc ccatgcgtct
    35401 ggaagtcttt tgtgaagacc gactcggtct gacccgcgaa ttactcgatc tactcgtgct
    35461 aagaggcatt gatttacgcg gtattgagat tgatcccatt gggcgaatct acctcaattt
    35521 tgctgaactg gagtttgaga gtttcagcag tctgatggcc gaaatacgcc gtattgcagg
    35581 tgttaccgat gtgcgtactg tcccgtggat gccttccgaa cgtgagcatc tggcgttgag
    35641 cgcgttactg gaggcgttgc ccgaacctgt gctctctgtc gatatgaaaa gcaaagtgga
    35701 tatggcgaac ccggcgagct gtcagctttt tgggcaaaaa ttggatcgcc tgcgcaacca
    35761 taccgccgca caattgatta acggctttaa ttttttacgt tggctggaaa gcgaaccgca
    35821 agattcgcat aacgagcatg tcgttattaa tgggcagaat ttcctgatgg agattacgcc
    35881 tgtttatctt caggatgaaa atgatcaaca cgtcctgacc ggtgcggtgg tgatgttgcg
    35941 atcaacgatt cgtatgggcc gtcagttgca aaatgtcgcc gcccaggacg tcagcgcctt
    36001 cagtcaaatt gtcgccgtca gcccgaaaat gaagcatgtt gtcgaacagg cgcagaaact
    36061 ggcgatgcta agcgcgccgc tgctgattac gggtgacaca ggtacaggta aagatctctt
    36121 tgcctacgcc tgccatcagg caagccccag agcgggcaaa ccttacctgg cgctgaactg
    36181 tgcgtctata ccggaagatg cggtcgagag tgaactgttt ggtcacgctc cggaagggaa
    36241 gaaagggttc tttgagcagg cgaacggtgg ttcggtgctg ttggatgaaa taggggaaat
    36301 gtcaccacgg atgcaggcga aattactgcg tttccttaat gatggcactt tccgtcgggt
    36361 tggcgaagac catgaggtgc atgtcgatgt gcgggtgatt tgcgctacgc agaagaatct
    36421 ggtcgaactg gtgcaaaaag gcatgttccg tgaagatctc tattatcgtc tgaacgtgtt
    36481 gacgctcaat ctgccgccgc tacgtgactg tccgcaggac atcatgccgt taactgagct
    36541 gttcgtcgcc cgctttgccg acgagcaggg cgtgccgcgt ccgaaactgg ccgctgacct
    36601 gaatactgta cttacgcgtt atgcgtggcc gggaaatgtg cggcagttaa agaacgctat
    36661 ctatcgcgca ctgacacaac tggacggtta tgagctgcgt ccacaggata ttttgttgcc
    36721 ggattatgac gccgcaacgg tagccgtggg cgaagatgcg atggaaggtt cgctggacga
    36781 aatcaccagc cgttttgaac gctcggtatt aacccagctt tatcgcaatt atcccagcac
    36841 gcgcaaactg gcaaaacgtc tcggcgtttc acataccgcg attgccaata agttgcggga
    36901 atatggctta agccagaaga agaacgaaga gtaagcgcga atatgcctga tggtgcaaca
    36961 ccatcaggca tattaactta tgctttcagt acagccagag ctgcttcgta atccggctcg
    37021 gtggtgattt catccaccag ctggctgaaa atcacattgt cattttcgtc aataaccaca
    37081 acggcacgcg ctgccagacc tttcagtggg ccatcagcaa ttgccacacc gtaggcttgc
    37141 aggaattcag cgttacggaa agtggagagg gtgataacgt tgttcagacc ttctgcgccg
    37201 cagaaacgag actgggcgaa cggcagatcg gcagagatac acagcacaac ggtgttgtcg
    37261 atctcagttg ccagttggtt aaacttacgt actgatgcgg cgcaaacacc ggtatcaata
    37321 ctcgggaaaa tgttcagcac tttgcgttta cccgcaaact gaccgagggt gacgtcagac
    37381 agatcttttg ccacgagagt aaaagtctgc gctttgctac ccgcctgcgg gatggaattg
    37441 gcgactgtaa ccgggttgcc ctggaaatga acggtttgtg acatgattat ctttcctgtt
    37501 tacatatagt taacgtcaca cctagtttat gctaactgtc aataacacag caaacgctat
    37561 ttgcgcttaa tccgcagacc accgcgacaa caaggagtaa agatgagaac cgttaaggta
    37621 tttgaggaag cctggccctt acataccccg tttgtgattg cccggggaag tcgcagtgaa
    37681 gcgcgcgtgg tggtggttga actggaagaa gagggtatta aaggcaccgg cgaatgcacg
    37741 ccgtatccgc gttatggaga aagtgatgcc tcggtaatgg cgcaaattat gagcgtcgtg
    37801 tcgcaattag agaaagggct gacacgggag gagttgcaaa aaattctccc tgccggcgca
    37861 gcacgtaatg cgctggattg tgcattgtgg gatctggccg cgcgaaaaca gcagcaatcg
    37921 ctggctgatt tgatcggcat aacgcttccc gagacagtta tcactgcaca gacggtagtc
    37981 atcggtacgc ctgatcagat ggccaatagt gcatcaacac tctggcaggc cggcgcgaaa
    38041 ttactgaaag tgaagctgga taaccatctt atcagtgagc ggatggtggc aattcgcaca
    38101 gctgtgcccg ataccacgct gatcgttgat gcaaatgaat cctggcgtgc agaagggttg
    38161 gcggcacgtt gccagctatt ggcggattta ggcgttgcga tgcttgaaca accgcttcct
    38221 gcgcaggacg atgcggcgct ggagaatttt attcatccgt tgccgatttg tgctgatgaa
    38281 agttgtcata ctcgtagcaa tttgaaggcg ctgaaagggc gctatgagat ggttaatatt
    38341 aagctcgata aaaccggggg tctgacggaa gcgctggcgc tggcgactga agcgcgtgca
    38401 aaaggtttca gtctgatgct gggctgcatg ttgtgtacct ctcgtgccat tagcgccgct
    38461 ttaccgctgg tgccgcaggt cagtttcgcc gatcttgacg gaccgacctg gctggcggta
    38521 gatgtggaac cggcgcttca gttcacgacg ggcgaattgc atctttagga tgccagcgca
    38581 gcaagtttgc catcgcaaag agatattttt cgctggcttc gtcggaggag atgggcggaa
    38641 actctgcggt gatgcaatgc aagttgagat ctgcacacca actgccgaaa gagccaggcg
    38701 tttcataacc gacgctggtt accagaggca attcaaacgc ctgagccagc cattcgccta
    38761 attcgctgtg tctgggatct tcaatacagg ccagtggatc gtggaatgag accacccagg
    38821 caggttgaat gcggtgtata agctggcaca gcgcctgggt ttcaggttcg gagccaggtt
    38881 tgtcaccagt cagtaatacg acatcacgtt cttcagcggc gctgttccag cgataaaccg
    38941 tttcaccttc cttccagttc gccgccggaa agtttctgtt taaatccaca ccattagcat
    39001 tggcccgtaa ccccagctgg cagccgtcag gattcacaca cagcaccaca tgatggcggc
    39061 gcaaagaagg tgtcaaagtc cgcagagcac aggagagcgt gacgaccgaa gagttttcat
    39121 caccgtgagt gccagccaga atcaaaccac tttcgcgact ggctgcaggg gccggaaacc
    39181 agattaacgg cgcgcccaat aatgaacgtc cgtaatgttc tgttccgggc ggaaatgcgc
    39241 cgcgttcggc gcgtgggcgg gttacggtca tgatcgtctc tggttcagga agtattacag
    39301 gcagtgttgt gcaaattttg cggattatca aatccttttc ttcaacgcta tgtcatcgat
    39361 cttgtatcac ctttttgccg gaagttgttt gtattttcat tgatgtgcag atattagaat
    39421 tgtcgtaatg ttatatacat ggcgtatttc attcttactg gttctacaca tgttaatcgc
    39481 atctgattga tcagttcacc ttaataagtt taataaatcg tactgcacat agtacagtac
    39541 gatggtagct atataattga tttacatgaa gaatggctta tcgccatcag taccaggttg
    39601 attgatgcct atattctcac caatgtgttt ctcaggttta tcgatgatat ctgtgattaa
    39661 tgcggcgaca ctcttacgcg atacgatcgt acctttgaat agttcattgc gtgaggtaag
    39721 ctcataatca ataatatctt catcagtcag ccaggcaggg cgaagaattg tataatccag
    39781 tccagaagcc tcaattgcat ccgcagccct gcggaatggt tttaggggct caccaataac
    39841 tgcattattc cattcgccaa attttcctgg aacttcatca taaatgccga gggaaagaac
    39901 aaagatcaga cgtttaacat cacaggcttt catcgcagcg atcactgaat ttgcctgaat
    39961 atctaaatct tcccccgtaa ggttggcata cacaatatcc tgtccctgca tggcctgctt
    40021 cagtgctgca tgattcagta catcccccat aataatctgg ctattagttg gatagggttt
    40081 gtgtattttt gctggctgac gagcaaataa agtttgttta atggtttgtt tatctgccag
    40141 ttgattaatc acatggcggg ctatctgacc gccagcacca agaatgagta catttttcat
    40201 aatatttctc atgaaactta tcacagatgg gtgttgaaaa aaggaataac tttttccagc
    40261 gcctcctgaa ctgcttccgg tttgtcatac agttcataat gtgagaaatc gggtaatgaa
    40321 actaactgac gatcctgcga aaccatcgcc cggccataga cttccagtcc atcacgataa
    40381 gcaccgaagg caccgacttt ttcaccaact actaccatca ccggctgagt cagtaacact
    40441 tcagtaaatg cgaaggcatc ccaggccagc gttttctgtg catgtgagaa caacattttt
    40501 gtcgcacctc ccggttgttg cccgcgtggt gttttatagt agtcagttgc ttcataaaca
    40561 tcgcgttcgg tcagtccatg ggcttttgct gcatccaggc tggcgggaag taattcgttg
    40621 atctgtaatt ctccaccacg agcctctttg gttcgttgcg acgccattgc attcaatgcg
    40681 ccaatcggat cgtaattgct gaaaccttct cgaaaaagac gcccgatatt aacacccgta
    40741 atactcacta cagctttaat tcgtttttct gtcagcgcgg cattaatggc atagccaccc
    40801 cccccacaca cacccagcac gccaatgcgt ttagcatcaa catatggcag agttactgca
    40861 taatcgataa cacggctaat atcctctacg cgctgtgtcg gatcttctat ccagcgaggc
    40921 tccccaccag attctccctg gaaactggcg tcataagcga gaacgacata gcctttttca
    40981 gccaatgcct tgccatagat attgccagac gtctgctcct tacaactgcc aaaaggatgg
    41041 acgctgataa tcgccggata ctgttttgac tcatcaaaat ttggtggaaa ctgaatattg
    41101 gcagcaatat gccaggccat atcactattg cggatttgaa tatgtttcat tacagagttt
    41161 cctcaaagaa cggagctaat actgaaattg cttcagccac ataggctttg ccgtcataaa
    41221 gatccatatg gtttgcgcct tcaacaatgt gatagcgttt atcctggctt gatgctcgat
    41281 cgtacaggtc gtcactcatc catttgctcc ctgcctggct gcccgccaca atctgcgtcg
    41341 gctgagtcag gtacacttcc gccatatggt aagcatcata ggtaataatc tggttaaggc
    41401 tgcgcaaagt agcgtaaccc ggtgctgttg gatactgcgc gcgaggggtg tggtaatact
    41461 cccaggcctg acgcagttct tcgttcggcg catcggcctc cttcattggt gccagtggca
    41521 taatggcata ttctccgctg ctaatatcgc tggttcgggc gtttgaaccc gcgtcaacgt
    41581 atggcagggc atcaatagat ttcacattgt tttcccaacc attacggaac atcgaaccaa
    41641 tattgaccgc actaacggta ccgatggcct tgatgcgacg atcctgaatt gcagcattgg
    41701 ctgtatatcc tgcaccggca caaattccca tcgcaccaat tcgggtattg tcgacataag
    41761 aaagcgttgt caggtaatca atcacggcac tgacgtcttc agtacgaatg tatgggtttt
    41821 ctaactgacg cggctcgccg ccactttcac cttgataaga tgcgtcataa gcaatagtga
    41881 caaatccctt ttccgccagt ttttcggcat aggttccggc cgtttgctct ttaacgcccc
    41941 cacctgggtg agataccaca attgccggat actgacgggt ttcatcaaat tttggaggga
    42001 aatagatcac tgcagacaaa gagatggttg gattattgct gttagtgaag ctgactttat
    42061 tattcatcat tccgttcctc atggagttgt cggttcgttt taacggttgg tgatatcact
    42121 atagatattg atcattaagt tgattagacc caaaatcatg attagaccta tcatttaaat
    42181 gattaataga ttgtatggag atactgaatg aagcgtgaag aaatcgctga tctgatggcg
    42241 tttgtcgtcg ttgcagagga gcgtagcttc actcgcgcag cagcccgcct gagcatggcg
    42301 cagtcagctt taagccagat agtgcgccgt atagaagaac gattgggatt gcggcttctg
    42361 acgcgaacta cgcgcagcgt tgtaccaact gaagcgggcg agcatctttt gtctgttctt
    42421 ggccctatgt tgcatgacat agattctgcc ctgacatccc tgagcgatct gcagaaccgc
    42481 ccatccggga caatacgtat tactactgta gaacatgcag caaaaacgat attgttacca
    42541 gcaatgcgca cattcctgaa atcgcatcct gaaattgata ttcagctcac cattgattat
    42601 ggtttgaccg atgtcgtttc tgaacgtttt gatgcaggcg tccgtctggg gggggagatg
    42661 gataaagata tgatcgccat tcgaatcggg ccagatatac caatggctat tgttggctca
    42721 ccggattatt tttctcgccg aagtgttcca acgtcagtgt cacaattaat agatcatcag
    42781 gcaattaatt tgtatcttcc cacatcgggt acagcaaatc gctggagatt aatacgcggt
    42841 ggacgtgaag ttcgtgttcg catggaaggt cagcttttac tgaatacgat agacctgatc
    42901 attgatgctg caattgatgg gcatggattg gcgtatctac cttatgatca ggttgagcgg
    42961 gctattaaag aaaaaaaact gatacgtgtt ttggataaat tcacaccaga tttacccggt
    43021 tatcacctgt actatccaca ccgtcgacat gctggctcgg cattctcatt atttatagat
    43081 aggctgaagt ataaaggtgc tgtttagcac tacttgctga tacattaatt taattcttct
    43141 cttaacgtat tctcagttcc tttcaacgtt ttggttattt tttattcttc gtacaatggc
    43201 gacagatgct gattatgata ccgaaaac
//
LOCUS       DAPGOF010000017        41457 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.017, whole genome shotgun sequence.
ACCESSION   DAPGOF010000017 DAPGOF010000000
VERSION     DAPGOF010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.017"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..391
                     /locus_tag="RHV13_000823"
     CDS             <1..391
                     /locus_tag="RHV13_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HDV1240379.1"
                     /translation="NKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLEALLAC
                     SQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGAGPEIT
                     TPWKSYQHEKNKNGLMPLIRRRTMLQL"
     gene            757..1824
                     /locus_tag="RHV13_000824"
     CDS             757..1824
                     /locus_tag="RHV13_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDV1240380.1"
                     /translation="MDKSKRHLAWWVVVLLAVAAIVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            1821..4556
                     /gene="rbbA"
                     /locus_tag="RHV13_000825"
     CDS             1821..4556
                     /gene="rbbA"
                     /locus_tag="RHV13_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HDV1240381.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVIHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPLLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            4556..5680
                     /locus_tag="RHV13_000826"
     CDS             4556..5680
                     /locus_tag="RHV13_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV1240382.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            5753..6028
                     /locus_tag="RHV13_000827"
     CDS             5753..6028
                     /locus_tag="RHV13_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HDV1240383.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            6025..6384
                     /locus_tag="RHV13_000828"
     CDS             6025..6384
                     /locus_tag="RHV13_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDV1240384.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(6504..6905)
                     /gene="nikR"
                     /locus_tag="RHV13_000829"
     CDS             complement(6504..6905)
                     /gene="nikR"
                     /locus_tag="RHV13_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HDV1240385.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(6911..7717)
                     /gene="nikE"
                     /locus_tag="RHV13_000830"
     CDS             complement(6911..7717)
                     /gene="nikE"
                     /locus_tag="RHV13_000830"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HDV1240386.1"
                     /translation="MTLLNVCDLSHHYAHGGFSGQHQHQAVLNNVSLTLQSGETVALL
                     GRSGCGKSTLARLLVGLESPAQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLNKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            complement(7714..8478)
                     /gene="nikD"
                     /locus_tag="RHV13_000831"
     CDS             complement(7714..8478)
                     /gene="nikD"
                     /locus_tag="RHV13_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HDV1240387.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(8478..9311)
                     /gene="nikC"
                     /locus_tag="RHV13_000832"
     CDS             complement(8478..9311)
                     /gene="nikC"
                     /locus_tag="RHV13_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HDV1240388.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(9308..10252)
                     /gene="nikB"
                     /locus_tag="RHV13_000833"
     CDS             complement(9308..10252)
                     /gene="nikB"
                     /locus_tag="RHV13_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001614015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HDV1240389.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHE"
     gene            complement(10252..11826)
                     /gene="nikA"
                     /locus_tag="RHV13_000834"
     CDS             complement(10252..11826)
                     /gene="nikA"
                     /locus_tag="RHV13_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HDV1240390.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLTAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADLRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(11937..12524)
                     /gene="acpT"
                     /locus_tag="RHV13_000835"
     CDS             complement(11937..12524)
                     /gene="acpT"
                     /locus_tag="RHV13_000835"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HDV1240391.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPEMPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(12579..13628)
                     /locus_tag="RHV13_000836"
     CDS             complement(12579..13628)
                     /locus_tag="RHV13_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDV1240392.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPIAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            13760..14977
                     /locus_tag="RHV13_000837"
     CDS             13760..14977
                     /locus_tag="RHV13_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1240393.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(14981..15538)
                     /gene="dcrB"
                     /locus_tag="RHV13_000838"
     CDS             complement(14981..15538)
                     /gene="dcrB"
                     /locus_tag="RHV13_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HDV1240394.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(15611..16276)
                     /locus_tag="RHV13_000839"
     CDS             complement(15611..16276)
                     /locus_tag="RHV13_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HDV1240395.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            16497..16742
                     /gene="tusA"
                     /locus_tag="RHV13_000840"
     CDS             16497..16742
                     /gene="tusA"
                     /locus_tag="RHV13_000840"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HDV1240396.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(16844..19042)
                     /gene="zntA"
                     /locus_tag="RHV13_000841"
     CDS             complement(16844..19042)
                     /gene="zntA"
                     /locus_tag="RHV13_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HDV1240397.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQSAEEPQASRLKENLSLITLIVMMVISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDLIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAALAIPAAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGVIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(19116..19742)
                     /locus_tag="RHV13_000842"
     CDS             complement(19116..19742)
                     /locus_tag="RHV13_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HDV1240398.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            19883..20242
                     /locus_tag="RHV13_000843"
     CDS             19883..20242
                     /locus_tag="RHV13_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HDV1240399.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(20245..20514)
                     /locus_tag="RHV13_000844"
     CDS             complement(20245..20514)
                     /locus_tag="RHV13_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HDV1240400.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(20504..21100)
                     /gene="rsmD"
                     /locus_tag="RHV13_000845"
     CDS             complement(20504..21100)
                     /gene="rsmD"
                     /locus_tag="RHV13_000845"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HDV1240401.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMERAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            21250..22746
                     /gene="ftsY"
                     /locus_tag="RHV13_000846"
     CDS             21250..22746
                     /gene="ftsY"
                     /locus_tag="RHV13_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDV1240402.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEDIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAAEVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELETQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            22749..23417
                     /gene="ftsE"
                     /locus_tag="RHV13_000847"
     CDS             22749..23417
                     /gene="ftsE"
                     /locus_tag="RHV13_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HDV1240403.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            23410..24468
                     /gene="ftsX"
                     /locus_tag="RHV13_000848"
     CDS             23410..24468
                     /gene="ftsX"
                     /locus_tag="RHV13_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HDV1240404.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            24713..25567
                     /gene="rpoH"
                     /locus_tag="RHV13_000849"
     CDS             24713..25567
                     /gene="rpoH"
                     /locus_tag="RHV13_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HDV1240405.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            25838..26941
                     /gene="livJ"
                     /locus_tag="RHV13_000850"
     CDS             25838..26941
                     /gene="livJ"
                     /locus_tag="RHV13_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HDV1240406.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            27091..27357
                     /locus_tag="RHV13_000851"
     CDS             27091..27357
                     /locus_tag="RHV13_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HDV1240407.1"
                     /translation="MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRA
                     AEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKRAAKRLQGM"
     gene            27364..27891
                     /locus_tag="RHV13_000852"
     CDS             27364..27891
                     /locus_tag="RHV13_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV1240408.1"
                     /translation="MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKIL
                     RAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQKKIPYKNIPSVTLGRLAIDRSL
                     QGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
                     FFPTKSIELLFTQSD"
     gene            complement(27888..28271)
                     /gene="panM"
                     /locus_tag="RHV13_000853"
     CDS             complement(27888..28271)
                     /gene="panM"
                     /locus_tag="RHV13_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HDV1240409.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGTLDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            28695..29804
                     /gene="livK"
                     /locus_tag="RHV13_000854"
     CDS             28695..29804
                     /gene="livK"
                     /locus_tag="RHV13_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HDV1240410.1"
                     /translation="MKRNAKTIIAGMIALAISHTALADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            29852..30778
                     /gene="livH"
                     /locus_tag="RHV13_000855"
     CDS             29852..30778
                     /gene="livH"
                     /locus_tag="RHV13_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HDV1240411.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            30775..32052
                     /gene="livM"
                     /locus_tag="RHV13_000856"
     CDS             30775..32052
                     /gene="livM"
                     /locus_tag="RHV13_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HDV1240412.1"
                     /translation="MKPMHIAMAMLSAAMFFVLAGVFMGVQLELDGTKLVVDSASDVR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRSAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            32049..32816
                     /gene="livG"
                     /locus_tag="RHV13_000857"
     CDS             32049..32816
                     /gene="livG"
                     /locus_tag="RHV13_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HDV1240413.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            32818..33531
                     /gene="livF"
                     /locus_tag="RHV13_000858"
     CDS             32818..33531
                     /gene="livF"
                     /locus_tag="RHV13_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HDV1240414.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            33928..35244
                     /gene="ugpB"
                     /locus_tag="RHV13_000859"
     CDS             33928..35244
                     /gene="ugpB"
                     /locus_tag="RHV13_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HDV1240415.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKNKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            35342..36229
                     /gene="ugpA"
                     /locus_tag="RHV13_000860"
     CDS             35342..36229
                     /gene="ugpA"
                     /locus_tag="RHV13_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HDV1240416.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            36226..37071
                     /gene="ugpE"
                     /locus_tag="RHV13_000861"
     CDS             36226..37071
                     /gene="ugpE"
                     /locus_tag="RHV13_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HDV1240417.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            37073..38143
                     /gene="ugpC"
                     /locus_tag="RHV13_000862"
     CDS             37073..38143
                     /gene="ugpC"
                     /locus_tag="RHV13_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HDV1240418.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            38140..38883
                     /gene="ugpQ"
                     /locus_tag="RHV13_000863"
     CDS             38140..38883
                     /gene="ugpQ"
                     /locus_tag="RHV13_000863"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDV1240419.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYGKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARELWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(38870..39310)
                     /locus_tag="RHV13_000864"
     CDS             complement(38870..39310)
                     /locus_tag="RHV13_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HDV1240420.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            39430..41172
                     /gene="ggt"
                     /locus_tag="RHV13_000865"
     CDS             39430..41172
                     /gene="ggt"
                     /locus_tag="RHV13_000865"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HDV1240421.1"
                     /translation="MIKPTFLRRVAIAALLLGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            complement(41210..>41457)
                     /locus_tag="RHV13_000866"
     CDS             complement(41210..>41457)
                     /locus_tag="RHV13_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HDV1240422.1"
                     /translation="YLDDADLPVVITLHGRFSQGWYFCFEAREFLETGDEAARLAGNA
                     PFIIDKDSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
BASE COUNT         9649 a        10683 c        11616 g         9509 t
ORIGIN      
        1 taataaaaag catcatgcta taattaatgg taaaaagttt aaaactagcc gggctatctc
       61 ttgtcgatcc tttaatcgtt atttttctgt ttcgacagat tacagaccta aacttgaagc
      121 actattagca tgctcacaaa tgttaggtat aaatcaagtt gttgcacata acggtaatgg
      181 tggaagagaa cgtataggtg aaacggggac agttttaggt cttaacgccc gtgacagtaa
      241 acatgctgga agaatgttca gcatgcataa ttgccaaatt aaccctggtg cggggcctga
      301 gataaccact ccctggaagt cttaccagca tgaaaaaaac aaaaatggac ttatgccttt
      361 gattagaagg cgcacaatgt tacagctctg aaccatagac aacatgaata ttaatgttac
      421 tatctggttg agactgttga taaaacgtaa taaggaatgc tctgttcctg caactgaaca
      481 ggcgtgaaac gcgatccaac aggatggata ccgttttgtc agccagtttt atagatcaca
      541 cttatgagta tgcgtgtttc atttacggcc aggaatgctg atgatgacaa cattactacc
      601 cgttttcact atgccttccc cgttggcgct caatgctctg cgcgctggcc gtatttgccg
      661 tttccttctt atcccggatg gaagaatccg ctgaacgtcg ttcggcggca gttgtaacac
      721 gcgtcgtttg cttacaggga ttatggcggg tcgattatgg ataaaagtaa gcgccatctg
      781 gcgtggtggg ttgtcgtgtt actggcggtg gcggctatag tggcgtggtg gctgttgcgc
      841 ccggcaggtg tgccagaagg ctttgccgtc agcaatgggc gcattgaagc gacggaagtg
      901 gatatcgcca gcaaaattgc cgggcgtatc gacaccattc tggtgaaaga aggccagttt
      961 gttcgcgaag gcgaagtgct ggcgaagatg gatactcgcg tgttgcagga acagcgactg
     1021 gaagccatcg cgcaaatcaa agaagcacaa agcgccgttg ccgccgcgca ggctttgctg
     1081 gagcaacgcc aaagcgaaac tcgcgccgca cagtcgctgg ttaatcaacg ccaggcagaa
     1141 ctggactccg ttgccaaacg tcatacgcgt tcccgttcac tggcccaacg aggggctatt
     1201 tctgcgcaac agctggatga cgatcgcgcc gccgctgaga gcgcccgagc tgcgctggaa
     1261 tcggcgaaag ctcaggtatc ggcttctaaa gcggctatag aagcggcacg caccaatatc
     1321 attcaggcgc aaacccgcgt cgaagccgca caagccactg aacggcgcat tgccgcagat
     1381 atcgatgaca gcgaactgaa agccccgcgt gacggacgcg tgcagtatcg ggttgccgag
     1441 ccaggcgaag tgctggcggc aggcggtcgg gtgctgaata tggttgatct cagcgacgtc
     1501 tatatgactt tcttcctgcc aaccgaacag gcgggcacgc taaaactggg cggtgaagcc
     1561 cggctgatcc tcgatgccgc gccagatctg cgtattcctg caaccatcag ttttgtcgcc
     1621 agcgtcgccc agttcacgcc aaaaactgtc gaaaccagcg atgaacggct gaaactgatg
     1681 ttccgcgtca aagcgcgtat cccaccggaa ttactccagc agcatctgga atatgtcaaa
     1741 acaggcttgc cgggcgtagc gtgggtgcgg gtgaatgaag aacttccgtg gcctgacgac
     1801 ctcgtggtga ggttgccgca atgacgcatc tggaactggt tcccgtcccg cctgtcgcgc
     1861 aactggcggg cgtgagccag cattatggaa aaaccgttgc gctgaacaat atcacactcg
     1921 atattccggc ccgttgcatg gtcgggctga ttggcccgga cggcgtcggg aagtcgagct
     1981 tgttgtcgtt gatttccggt gcccgcgtca ttgaacaggg caatgtgatg gtgctgggcg
     2041 gcgatatgcg cgacccgaag catcgccgcg acgtctgccc gcgcatcgcc tggatgccgc
     2101 aggggctggg caaaaatctc taccacacct tatcggtgta tgaaaatgtc gattttttcg
     2161 ctcgcctgtt cggtcacgat aaagcggagc gggaagtgcg aattaatgag ctgctgacca
     2221 gcaccgggtt agcaccgttt cgcgatcgtc cggcagggaa actctccggc gggatgaagc
     2281 aaaaacttgg gctgtgctgc gcgttaatcc acgacccgga actgttgatc cttgatgagc
     2341 caacaacggg ggttgacccg ctctcccgcg cccagttctg ggatctgatc gacagtattc
     2401 gccagcggca gagcaatatg agcgtgctgg tcgctaccgc ctacatggaa gaggccgaac
     2461 gcttcgactg gctggtagcg atgaatgccg gagaagtgct ggcaaccggc agcgccgaag
     2521 agctgcggca gcaaacgcaa agtgccacgc tggaagaggc gtttataaat ctgttaccgc
     2581 aagcgcaacg ccaggcgcat caggcggtag tgatcccacc atatcaacct gaaaacgcag
     2641 agattgccat cgaagcgcgc gatctgacca tgcgttttgg ttccttcgtt gccgttgatc
     2701 acgtcaattt ccgcattcca cgcggggaga tttttggttt tcttggttcg aacggctgcg
     2761 gtaaatccac caccatgaaa atgcttaccg gattgctgcc cgccagcgaa ggtgaggcgt
     2821 ggctgttcgg gcaaccggtt gatccaaaag atatcgatac tcgccgtcgg gtgggctata
     2881 tgtcgcaggc gttttcgctc tataacgagc tcaccgtgcg gcaaaacctt gagttacatg
     2941 cccgtttgtt tcacatcccg gaagcggaaa ttcccgcaag agtggctgaa atgagcgagc
     3001 gttttaagct caacgacgtt gaagatgttc tgccggagtc attgccgctc ggcattcgcc
     3061 agcggctttc gctggcggtg gcggtgattc atcgcccgga gatgttaatc ctcgatgagc
     3121 ctacttctgg cgtcgatccg gtggcgaggg atatgttctg gcagttgatg gtcgatctct
     3181 cgcgccagga caaagtgact atcttcatct ccacccactt tatgaacgaa gcggaacgtt
     3241 gcgaccgcat ctcactgatg catgccggaa aagtgctcgc cagcggtaca ccgcaggaac
     3301 tggttgagaa acgcggagcc gccagtctgg aagaggcatt tatcgcctat ttgcaggaag
     3361 cggcaggaca gagcaacgaa gccgaagcgc cgcccgtgat acacgacacc acccacgcgc
     3421 cgcgccaggg atttagcctg cgccgtctgt ttagctacag ccgccgcgaa gcgctggaac
     3481 tgcgacgcga tccggtacgt tcgacgctgg cgctgatggg aacagtgatc ctgatgctga
     3541 taatgggtta cggcatcagt atggatgtgg aaaacctgcg ctttgcggtg cttgaccgcg
     3601 accagaccgt cagtagccag gcgtggacgc tcaatctctc cggttcccgt tactttattg
     3661 aacagccgct gcttaccagt tatgacgagc ttgatcgtcg gatgcgtgcg ggcgatatca
     3721 cggtggcaat tgagatccca cccaatttcg ggcgcgatat cgcgcgtggt acgcccgtgg
     3781 aactcggcgt ctggatcgac ggtgcaatgc cgagccgtgc cgaaacggta aaaggttacg
     3841 tgcaggccat gcaccagagc tggctacagg atgtggcgag ccgacaatcc acgcccgcca
     3901 gccaaagcgg gctgatgaat attgagacac gctatcgtta taacccggac gtaaaaagcc
     3961 tgccagcgat tgttccggcg gtgatcccgc ttctgctgat gatgattccg tcaatgctaa
     4021 gcgcccttag cgtggtgcgg gaaaaagagc tagggtcgat tatcaacctt tacgtgaccc
     4081 ccaccacccg tagcgagttt ttgcttggca aacagctgcc gtacatcgcg ctggggatgc
     4141 tgaacttttt cctgctctgc ggtctgtcgg tgtttgtgtt tggcgtaccg cataaaggca
     4201 gtttcctgac gctcaccctg gcggcgctgc tgtatatcat catcgccacc ggaatggggc
     4261 tgctgatctc cacctttatg aaaagccaga ttgccgccat tttcggtacg gcgattatca
     4321 cgttgatccc ggcgacacag ttttccggga tgatcgatcc ggtagcttcg ctggaagggc
     4381 cgggacgttg gatcggcgag gtttacccga ccagtcattt tctgactatc gcccgcggga
     4441 cgttctcgaa agcgctggat ctgactgatt tgtggcaact ttttatcccg ttgctgatag
     4501 ccataccgct ggtgatgggc ttaagcatcc tgctgctgaa aaaacaggag ggatgatgcg
     4561 ccatttacgc aatattttta atctgggtat caaagagttg cgcagtctac tcggtgataa
     4621 agcgatgctg acattgattg tcttctcgtt tacggtgtcg gtgtattcgt cagcgaccgt
     4681 tacgccagga tcgttgaacc tcgcgccgat cgccattgcc gatatggatc aatcgcagtt
     4741 atcgaaccgg atcgttaaca gcttctatcg cccgtggttt ttgccaccgg agatgatcac
     4801 cgccgatgag atggatgccg gactggacgc cgggcgttat acctttgcga taaatattcc
     4861 gcctaatttt cagcgtgatg tcctcgccgg acgccagccg gatattcagg tgaacgtcga
     4921 tgccacgcgc atgagccagg catttaccgg caatgggtat atccagaata ttatcaacgg
     4981 tgaagtgaac agctttgtcg cgcgctaccg tgataacagc gaaccgttgg tatcgctgga
     5041 aacccggatg cgctttaacc cgaaccttga tcccgcgtgg tttggcgggg tgatggcgat
     5101 catcaacaac attactatgc tggcgattgt attgaccgga tcggcgctga tccgcgagcg
     5161 tgaacacggc acggtggaac acttactggt gatgccgata acgccgtttg agatcatgat
     5221 ggcgaaggtc tggtcgatgg ggctagtggt gctggtggta tcgggattat cgctggtgct
     5281 gatggtgaaa ggcgtgctgg gcgtaccgat tgaaggctcg atcccgctgt ttatgctggg
     5341 cgtggcgctc agtctgtttg ccaccacgtc aatcggcatt tttatgggga cgatagcgcg
     5401 ttcaatgccg caactggggc tgctggtgat tctggtgctg ctgccgctgc aaatgctttc
     5461 cggtggatcc acgccgcgcg aaagtatgcc gcagatggtg caggacatta tgctgaccat
     5521 gccgacgaca cactttgtta gcctcgcgca ggccattctc taccggggtg ccggattcga
     5581 aatcgtctgg ccgcagttcc tgacgctgat ggcaattggc ggcgcgtttt tcaccattgc
     5641 gctgctgcga ttcaggaaga cgattgggac aatggcgtaa ggaagggaaa cttgacttca
     5701 tactgtgatg tcatactatt gatgtcataa agtgaagtca taaggacatg ccatgagaac
     5761 caaggtacag gctttacgga agaaacaaaa aaatacattg gatcaaatat ttaaaactcc
     5821 tgttcctcaa gggatcaagt ggtctgatat agagtcactg gttaaagcat taggcggaga
     5881 aattaaggaa ggaagaggtt cgcgttgtaa gttcatacta aatatgagcg ttgcgtgttt
     5941 ccatcggcct catccgtcgc cagataccga taaaggcgct gtagaaagcg tgcgtgactg
     6001 gttactaagt ataggggtaa aaccatgatc aaactgaaga cgccaaactc tatggagatt
     6061 gccgggcaac ctgctgttat cacttacgtg ccagaactga atgcatttcg tggcaaattc
     6121 ctgggcttgt caggttattg tgattttgtc tctgacagca ttcaggggct gcaaaaagaa
     6181 ggggaacttt ccctgcgaga gtatcttgag gattgtaaag ctgcgggtat tgagccgtat
     6241 gcgcgtacag aaaagattaa gacctttacg ttacgctatc cagaatcgct gagtgaacgc
     6301 cttaataatg cggctgcgca gcaacaagtc tctgttaata cctatattat tgaaacactg
     6361 aatgagcgtt tgaatcattt ataaatttta gcggcgtaca ttacgcagaa tatctgcctt
     6421 tacaggatct atctatgcaa ctgaacttaa ttttataaat taagttcagt taatatcttt
     6481 tctttcctcg ttttagaaat aactcaatct tccttcggca ggcactgcaa atgcccgtgc
     6541 cgcacgccgc gctgggcgat gacgtcatcg gcaaaatgct gcacgtcacc catgtcacct
     6601 ttcagcacgg cgatttccag acagtcgtcg tggttgatat ggacatgcag cgtggcaacc
     6661 gagaggtcgt ggtgatgatg ctgggtggaa acaatacggc tggctaagtc gcgtttttcg
     6721 tgttcataca catacgacag caccgcgaaa ccttgtgtgc cgtgctgctg ggtagcttcc
     6781 tgcgccaggg cactacgcag aatatcgcgg atagcttcgg aacggttgtt ataaccacga
     6841 cgctggctca ggctgtccag cgtctccagt aaatcgtcat caagcgtgat ggtgactcgt
     6901 tgcatttgcg ttaaaccttt tctgaggtgc gacggcgcac ggggaatgcg ggtaataccg
     6961 cgttttgtag cacacgtccg gcgtcagagg aaaaggttaa tttatctccc accgcctggg
     7021 tttcgacgat ttgtccgttg tccatcacca tcactcgctg gcaaaagcgt tccaccaggc
     7081 gtaagtcgtg ggtgatgaac aggcaggcgg tgccaaactg ttgttgtagc tttttcagca
     7141 ggcgaatgac gcccgcctgt aacacgagat caaggttaga aactgcttca tccagaatca
     7201 gcagtttcgg ttcgaccgcc agcgcgcgag ccaggcagac gcgctggagc tggccgccgc
     7261 ttaactgcgg tgggcgcttg ttgagcacgc tgtcgtcgag atcgaccgcc ttcagcattt
     7321 cgctggcgcg cgccagttgt tcggattttt tcagtgatag caggtggcgc atcggttcac
     7381 gcaggatctc gcgcacggtt ttgcgcggat tcacggcgct gatggagtcc tgaaatacca
     7441 tctgaatatc gcgacggaat gctttacgtt gcgcgcgatt aagtttcgcc agcggttcgc
     7501 cacgccagct aatattcccc tgcgcgggcg attccaaacc taccagcagc cgcgcgaggg
     7561 tgctttttcc gcagccgctg cgtcccagca gggcgacggt ttcgccgctt tgcagggtca
     7621 gggaaacgtt attcagcacc gcctgatgtt gatgttgtcc gctaaatcca ccgtgggcat
     7681 agtgatggga aaggtcgcag acgttaagta aggtcatgat gccagctcca taccgtagag
     7741 ggcgagatga gcggaaacca ggctgcgcgt caccgtatgt ttgggggcgt taaacagcgt
     7801 ttcgacatcg ccctgttcga caattttacc ttgtgacatc accgccacgt catccgccag
     7861 acgcgccaca acgcccatat catgggtgac cagcagcatt cccggcgctt gtttttgcat
     7921 aatgctttcc agcagatcga ggatgcgcgc ctgtgctacc acgtcgaggt cggtggtcgg
     7981 ttcatcggcg atgataaacg gtgattcaca cagcaccgcc atcgcaatca tcatgcgctg
     8041 caacatgccg ccgctcatct cgaacgggta cagcttcagc acgcgcgcgg cgttttccag
     8101 ccccaccgct tctatggcag cggtaagcgt agcgtcatcg gcgggtttcc ctaacgccag
     8161 gcaggtttcc cgcgcgtggg tgtgcatggt gtgcagtgga ttaaaggcgc tgcgcgggtt
     8221 ctgcatgatg gtggcaattt tgatgccgcg cagggcgcaa ggcgaaaccg gtttgccatc
     8281 ggctaaaatt tcccccgccg tctggcgaac gccagcgggc agaatgccca gcgtcgcggc
     8341 gcaggtcagt gatttcccgc tgccgctacc gccgactaac gccagcacac gcccgcgttt
     8401 aagggttaac gatacgccgt gcaccagcgg ctgcgcggcc tgtagcgcga tattacgcag
     8461 ttcaatctgt tgcggcatta gtgtgcgtgc tccgtcacca gatgaggatc cagatgatcg
     8521 cgcagtgcgt cacccaccag gttaaaggcc atcacgctga taaacagcgc cagccccggc
     8581 cagaacattt gcagcggctg ggtccagata tactggcgcg cgtcgttaat catcacgccc
     8641 cattcggcgg tcggtgcggt cacgccgagg ccgaggaagg acatccccgc gacgtgcagc
     8701 atcatatggc cgatatccag cgttgccagc accagcagcg aagggatcac cgcacctgcc
     8761 agatgatcaa caaacactcg cacatggccc gcgccagaaa gccgtgatgc cagcacaaac
     8821 tcgcgctggc gcagtgaaat caccaggctg cgcaccatgc gtgcatacca cgcccagtgc
     8881 gacagggcga tggcgataat tacgttggtc agcccagtgc cgagcacgcc aaccataaag
     8941 aacgacagaa tcgaggtcgg gaaggtcata aacatatcgg cgacgcgcat ggtagcctga
     9001 tcaacgcgcc cgccaatcag cccggcgctg ccgccaataa ccagccctaa tgtcagcacc
     9061 agcagcaggc aggccattac cgaaccgagc gacacgcggg tcgctgccat cagccgcgag
     9121 aaaatatcgc gccctaagtg atcggtgccc agccagtgct gcgcatccgg cgaaagcagg
     9181 cgcgacggca aatcaatcgc ctgggggtca tacggcagcc accactggct ggtgagcgca
     9241 atcagcgcca gcagggcgat aatgatcagc gccaggcgta ccgaccagcg ggaagagagg
     9301 aaaaagttca ctcgtgcgct ccttcatgac gacgaatgcg cgggtccagc gcggcgttga
     9361 gcaaatcgac aatcaaatta cagaccacaa aaaccaccac catcatcagc gtaaagcact
     9421 gaatcaccgg atagtcacgg ttaaaaatcg ccgacaccgc atagcgcccg acgcccggcc
     9481 aggcaaagat gttttcgata atcatcgtcc cgccaatcag ttcgccgatg tgcatcccca
     9541 cggcggtgat catcggcagc gaggcattgc gcagaatatg gcggcgttcg gtctgtttgt
     9601 cgttcaggcc gcgcagacgc gcccaggtga cgtgacgctg accggcgacg tccagcatac
     9661 tggcgcgcag taaacgcgcg ttaatcgcca gcgacataaa ggcaatggaa accgcaggca
     9721 aaatgatgtg ctgccagccg ccgtagccca tcgcgggtag ccattgcaga tacaccgaaa
     9781 acgccatcac cagtaaaaac gccagccaga agttaggcat cgacacgcca agaaatgcga
     9841 tgaaacgtac ggcgaaatcc ggcagacggt cgcgatggcg cgccgcccag ataccgagcg
     9901 gtacggaagt gagcagaatt aataccagcg ccgcacctgc aagttccagc gtggcgggca
     9961 ggaagttcag catatcgtcc agtaccgggc gttggctggc gaatgagata ccaaagtcaa
    10021 gatgcagcgc cttccacaac caggtgccgt actggacgta cagcggctga tccagtccca
    10081 gcatggtgcg ggtagaggcc agcatctccg gcgtcggcgg caggttagac agacgcaaat
    10141 aatcgagcgc cgggtcgccg gtaccgaggc gcagcatcag aaaaatgatc accgaggcgg
    10201 cgagcaccat cgggatcagc agcagaaagc ggcgtaatac gtaacgcaac attaaggttt
    10261 caccggttta atctgttcga acggaatttc ggtggcgatc ggcgcgtagg ggatgttacc
    10321 cagctccggt tttgatacca ccatcattga gatgtaactg ataggcagat aaaccgcctc
    10381 gtcatgcaga cgggtcagaa tgtcgcgata cagcgcctga cgttgcgttt cgtcatgggt
    10441 cgccagcact tcgccgatct ctttatcaat cagcggtttg tcggctaatc cttgctgtgc
    10501 ctggaagtca gcgtgtgacg gtacgcgcat tgaactgagg aaggcgtgtg gatcgtatgg
    10561 cgcgccccag gtgcggtgga aaatcatgcc aaaacgaccg tcgcgctgac gagcatagat
    10621 actgctctct tcttcgccaa tcagcgagac atctgcgcca atctggcgca gatcagcctg
    10681 aatgatttcc gccatcgatt tgcttaacgc atcggtgccg atgaacgaca gttcaatgcg
    10741 cagcggctga ccatttttct cgcggatgtc tttgcccgca gtcagcgtcc aaccggcttt
    10801 ttccagcaac tcttttgctt tctgcggatc gtactggcgc ggtttcaggc cgaggttggc
    10861 gtagggcaca gaaggggcaa acagggtgtc ggcgacctgc tgggtgccat acaacgcgtt
    10921 atcaatcagc gattttttgt ttaccgcgta attaagagct tcacgtactg ccagctcgtt
    10981 ggtgggggct ttggcggtat tgagcgccag catcacggtt tcgatcggct gtgacagttg
    11041 ggtgtggtaa gccggattct ggctaaagcg ggcgaaggta tcgagcggta ataacccttc
    11101 gtttccgtac agcaggtcga tatcgccagt ttcaaacgcc accgcgcggg tagtcgggtc
    11161 cgggatgacg ttaaaagtga tcttcttaat cgccggcttt tcgccccagt agttttcgtt
    11221 acggacgaag acatcgtact gattcagttt cgattcctgc aaaatccacg gtccggtgcc
    11281 aatcggcgct ttaattccgt tcatggtttc atggttttta aactgcgagg gggcgataaa
    11341 acggaaaggg cgcggcaggg ccagttcttg caggaaagga tagtaggcgc ttttcagggt
    11401 gatttgcagc tctgttttac tgagtgcttt aacatcaaca atctggtttg ccagctccag
    11461 ccaggcgtga cgttgacggt tatcgagcac tgcgcggaag ttttctgccg ccgcctcggc
    11521 atcgaacggt tcaccgttgg agaatttcac gtcatcacgc agggtgaagg tccaggtttt
    11581 accatcttct gaatgagtcc agctttttgc cagccacggg atcaccgaac cgtctgcctg
    11641 atatttcacc aatggttcat aaaccatgct ctgggcgaac atctggttag gcgtgtaaag
    11701 gtgcgggttt agtggcccga cattcaccgg ccaggcggtg gtgatttcat ctggtgcagc
    11761 ggcatggacg ataaaagacg cacaagccag cagcgcaaat agagtgcggc ggagtgtgga
    11821 gagcatggta accccaatgg attaaaatag atggcggaaa taagtatgac gattttaagt
    11881 attcgtcata ctgattacct gttctggatc aatagatggg cagtcgggtg ggcggatcag
    11941 ttaactgaat cgatccattg cacactgtcg gcggtgagcg taaagggggt gggggtgcag
    12001 atcgcaaggc ttaaattttc aagctgacaa tggctgactg acagcgagga gtgataggtg
    12061 ctgtctacgc tgacgatttg ccaggcgctg ccgccacgct gtttaacgat ggcttctttg
    12121 cgcgtccaga tgcgccagaa catttccagt tgctgatcag gatgcacggc gtccatctca
    12181 gcgtgttccc cgaggctgaa tacggcgttc gccagccagc gccagttggc gcgcgggcga
    12241 atcacttcga tatcgcagcc gacttcgcct tcatcactca acagcagggc gatatcgtca
    12301 ccgctatggc ttaagttgaa ccatagcggc atttccggcg caaatgcagg tttgccttgt
    12361 tcgccataga tgatctccgg tagcggggaa agcgtgtgcg aaagcaatgc acgccccgcc
    12421 agccagcgtt cgcgtcgtgg accttgcggt gcttgctcgc gtaaacccgg tggcagtgga
    12481 gctgcgctta aggtcgaaac tttccccaga actatccgat acatatcagg gccaacgttt
    12541 aatggaaaat gaaagtgcgt atcgtatcac ttgtcgcctc atcccggtaa ccgacttttc
    12601 ggtctgcccg gccccagtaa aatcgccagt ttgctgccac ctttcgtcgt ttccatccag
    12661 attttacaca cgctggttaa tggcaccgaa agtagcatcc ctaccgggcc gagcagccat
    12721 ccccagatta acaatgaaag aaataccacc atggtggaca tccccaggcg atggcccatc
    12781 atccgtggtt ctaaaatatt gccgatgacc atatggacca ctaaaaacaa tgcgccgacc
    12841 agaatacatt cgtaaacacc attaaacagc agcacctgaa tcattggcgg tacggcggaa
    12901 attaccgcgc cgatattggg cacgtagttg agcaaaaacg ccagtactgc ccacatcagc
    12961 gcaaactgca cccccatcag ctccagcccc agccagacga tgacacctgt ccataaactg
    13021 agtagcgtct tcaatgcaag atagtgcgaa acgcctttaa gtgcgcggtg taatcctgcg
    13081 atgtgaatct gtgggttatt cagcgcaaaa cgcattttgt aagggacgtg gcgcacttca
    13141 aacagcataa aaactacggt catcaccagc aaaagcacgc tcgccattgc cccggaaagc
    13201 ccggtcatta gcgctgtggt gaaggtaacc actttttccg agtccatccg ctgcagcatt
    13261 cgctccggcg acatatgcaa attaagaaaa ggcaacatct cctgcaattt aaaaagtttg
    13321 cgcgtcaact ccttattaaa cttcggcagc atagagataa attcgttaaa cgatgccgcc
    13381 agtacgccga ccagcgcagt tagtgcgatc agcatcacca ccaccacaat cgtaatggca
    13441 atggggcgtt gtactccccg acgaataaac caggtgacga gcgggttgag gacgatggca
    13501 aaaaacagcg ccagtaacag ctgcacaatg atatctgccg ctgcgtgaat gcccgcgagg
    13561 atcactacca gcgaggccag cttgagcaga atgtgcatgc ccgttttatc gggttgaggg
    13621 gtttccattg gggcttcctt gtgacttttt gtattaagtg tagcgggagt cacgccagca
    13681 atattctgat tcttactgct gattttcacg tcagcacgtg gtaaaaatga aacactgttg
    13741 taaaaatgtg gtgatcctca tgcccgaacc cgtagccgaa cccgcgctaa acggattgcg
    13801 cctgaatttg cgcattgtct ctatagtcat gtttaacttc gccagctacc tcaccatcgg
    13861 gttgccgctc gctgtattac cgggctatgt ccatgatgtg atgggcttta gcgccttctg
    13921 ggcaggattg gttatcagcc tgcaatattt cgccaccttg ctgagccgcc ctcatgccgg
    13981 acgttacgcc gatttgctgg gacccaaaaa gattgtcgtc ttcggtttat gcggctgctt
    14041 tttgagcggt ctggggtatc tgacggcagg attaaccgcc agtctgcctg tcatcagcct
    14101 gttattactt tgcctggggc gcgtcatcct tgggattggg caaagttttg ccggaacggg
    14161 atcgaccctg tggggtgttg gcgtggttgg ctcgctgcat atcgggcggg tgatttcgtg
    14221 gaacggcatt gtcacttacg gggcgatggc gatgggtgcg ccgttaggcg tcgtgtttta
    14281 tcactggggc ggcttgcagg cgttagcgtt aatcattatg ggcgtggcgc tggtggccat
    14341 tttgttggcg atcccgcgtc cgacggtaaa agccagtaaa ggcaaaccgc tgccgtttcg
    14401 cgcggtgctt gggcgcgtct ggctgtacgg tatggcgctg gcactggctt ccgccggatt
    14461 tggcgtcatc gccaccttta tcacgctgtt ttatgacgct aaaggttggg acggtgcggc
    14521 tttcgcgctg acgctgttta gctgtgcgtt tgtcggtacg cgtttgttat tccctaacgg
    14581 cattaaccgt atcggcggct taaacgtagc gatgatttgc tttagcgttg agataatcgg
    14641 cctgctactg gttggcgtgg cgactatgcc gtggatggcg aaaatcggcg tcttactggc
    14701 gggggccggg ttttcgctgg tgttcccggc attgggtgta gtggcggtaa aagcggttcc
    14761 gcagcaaaat cagggggcgg cgctggcaac ttacaccgta tttatggatt tatcgcttgg
    14821 cgtgactgga ccactggctg ggctggtgat gagctgggcg ggcgtaccgg tgatttatct
    14881 ggcggcggcg ggactggtcg caatcgcgtt attactgacg tggcgattaa aaaaacggcc
    14941 tccggaacac gtccctgagg ccgcctcatc atcttaaaac ttactgaata accagcgtat
    15001 taatgatgtt ttctgcggtg gtctgcgctt tttgctgatc gtcagcgggc agagtaattt
    15061 gcatggtcag cagttgatta cccacgttac ccagaataac ggaagagtac gccgtctggc
    15121 ctttcgcgga gataatactg tctaactgct gcattttgtg acctttcagc tcaatggctt
    15181 tattggttac cacttgcagc tgcggatcgc ggctacgttg ctgatcttcc agacgcttcg
    15241 ccagcaccgc cagatcttct ttcggatcat cgcccatgat gacgatgact gctttctgcc
    15301 cggtggcgtc ggaccagaca tgcatgttat tggcctgcgt tcccagctta ccgctctggt
    15361 cggtcatatc cgctggcagc gagaaactta acttgccatc aagcaggttg acgggattcc
    15421 cggtagcgtt actttccgcg accgaaccct gcgccgtagc gttagtgtct ttatcatcac
    15481 aggccgcaag ccccataacc agcaggccaa ttccgacata tttaaccaga ttgcgcattg
    15541 acttcttcct ttcgataaac ggccataacg gctcattcat ccatcttatc acaactctga
    15601 taacgaacct ttaactcgcc tgcaaagcgt tgatttcgga tttatctgcc agtcttttca
    15661 acagcatatt gagtaatacg ccatacattg gcaggaagaa aacgatactg attaacactt
    15721 tgaaacagta atcgaccagc gcgatttcca tccagtgttc agccataaag gcatccgggc
    15781 tacgccagaa ggcaatgaag aaaaaggcca gcgtgtcgct gacgttaccg aacagtgtgg
    15841 acgctgtcgg tgccagccac cagcggcgac tctgacgcag gcggttaaaa acgtgcacgt
    15901 cgaggatttg ccccagcgcg taggccatga aactggcggt ggcgatacgg gcgacaaaca
    15961 ggttgaagtg ggcgagtgcg ccgaatccct gccatgaacc catatagaac agcgacgaga
    16021 tgacatagga gattaataac gcagggatca ttaccgcgaa gataatgcgt cgggccagcg
    16081 gtgcgccaaa aatacgcacg gtcaggtcgg tcgcgagaaa gataaacgga aaactaaacg
    16141 cgccccaggt ggtatgaaaa ccaaaaatgg agaccggaag ctggaccaga tagttactgg
    16201 aggtgatcac cagcagatga aataacgata accagaacaa cgccttatag cgttgagttt
    16261 gcgagaaaac gttcatattg tacctttttg attaaccatt ggggtgaggg aacccaatac
    16321 gtacgacacg tctgttatct taacataata acgtgcttac ccttttttcg agccgccgca
    16381 tgatactgct ttacgttgac aatgcaatgg ttaattttca cgcaatcgtt aacctggttt
    16441 gcttacggac tcacagggcg taaactagcg ccgttttttt aagtgatgag aagaaaatga
    16501 ccgatctctt ttccagccct gaccacacac tcgacgcgct tggcctgcgc tgcccggaac
    16561 cggtgatgat ggtgcgcaaa accgtgcgca atatgcagcc tggcgaaact ttactgatta
    16621 tcgccgacga tccggcgacg acccgcgata ttcctgggtt ttgtaccttt atggaacacg
    16681 aactggttgc gaaagagacg gatggactgc cttatcgtta tttgattcgt aaaggcggtt
    16741 gatggggaaa gattggcttc gatgccgcct tttcccctca ccctaaccct ctccccagag
    16801 gagcgagggg accgatcgcg ctcaatgttg cgatcggttt gccttatctc ctgcgcaaca
    16861 atcttaacgc attcgctgtt accagcacgg tcgcaccggt atctgccagc accgccagcc
    16921 atagtccggt catccctaac agcgtggtga cgaggaagat ccctttcagc cccagcgcaa
    16981 tagtgatgtt ctggcggata ttggcgtgag tggcgcgtgc cagttcaatc atttgcacca
    17041 ggccgcgcag gtggttatgg gttaatgctg cgtcggcggt ttccagcgcc acgtctgtgc
    17101 cactgcccat tgcaatccca atggcggcag ctttcatcgc tggcgcgtcg ttaataccat
    17161 caccgaccat cgccaggggc gcatgttgat tcagcttggt caccgctttg actttatctt
    17221 ccggcaatag gcccgcttta aactccagcc ccagctcccc ggcaattgcc gccgctgcgc
    17281 gtggattatc gccggtgagg atcacccctt tgacgcccag cgcgttcagt tcactgatgg
    17341 cagttgcagc atcggcgcgc agggtatcct gtaacgcaat gacaccaagc acgtcatcgt
    17401 tacgtactac cagcactacc gtttgcccgg cgctttccag ttcgttaatt aaaccagcaa
    17461 atgcatcagc gggatgtttc ccggcagcgc aaatcaatac gcgctcaccg ttaacctgcg
    17521 cttcaatgcc agacccgacc agcgcccgct gtgattcggc ggcgggaatg gcgagtgcag
    17581 caacctgtgc ttcacgcacg atggcctgcg ccagtggatg cgtcgcgcct tgctcgaccg
    17641 ccgccgccag tgtcagcagt tcagattcac taatacccgt tgccggatga atcgcggtaa
    17701 cgcgcggttt accgacggtc agcgtaccgg ttttatcaaa cgccacctga gtaacacgac
    17761 ctagttgttc cagcgccgct ccgcctttaa tcaacgcccc acgacgtgcc gctgctgcca
    17821 gcccggaggt aatcgccgcg ggcgtggaga taactaacgc acacgggcag ccaatcagca
    17881 gcagcgtcag ccctttataa atccactctt gccagctggc ggcaaacagc agcggcggca
    17941 ccagcgttac cagcagggcg acggccataa tcgctggcgt gtaaatacgg ctgaaacggt
    18001 cgataaaccg ctcaatgggg gcgcggcgct cttcggcttc ttcgatcagt ttcagaatcc
    18061 ggtcaatggc gctggcaccc ggttctgaca gcacttccag cgttaccaga cggtctacgc
    18121 tggtggctcc tgcaggaact ttatcgcccg tcgcgcgctc caccggaatg gattctccgg
    18181 tcagggcgct ttcatcaaaa ctggcaaacg gtgagagcag tttaccgtcg gcaggcaaac
    18241 gcccacctgc ggcgacttca atcaaatcgc caggccgcag gctgttaatc gccacctctt
    18301 cccgctcacc gttacgcagg cgcgtggcgg tttctggttt cagcgccatt aacgcgctaa
    18361 cgccctgacg cgcgcggctg gcggcccagc cttccagtcg ttcaccaatc aaaaacagca
    18421 gcaacaccat cgcagcttca gccgttgcgc caataaacag tgcaccaata gcggctacgc
    18481 tcattaaggt ttcaatggcg aagtagctgc cggatttgat cagccgtaat gcctgacgag
    18541 caatcgggta cagcccaacc agcgtggtcg cgataaacgc cagttgcccg aacggatgat
    18601 tgaactgctc cagaccccag ctgattacca tcatcacgat aagcgtaatc agcgacaaat
    18661 tctctttcag gcgtgatgct tgcggttctt cggcggactg ctcatcgcgc agggaatagc
    18721 ccgctttttg caccgcagat tcaacttgtg cacgaatgtc attgtcggca tcaaccacca
    18781 gtttttcggt ggcgaacaac acctgcacct gattcacacc tgcaagctgg cgcacggcat
    18841 tttctacctt gcgcgcacag gcggcgcagt ccatgccact gactttccag ctatagcggg
    18901 tgccggagac gttttcagag agcgttggcg tgctggaaca tgcgccgtcg cagcaacagt
    18961 cgttggcgtt ctgtaccgtg gttagcggtt tgaacgcagc aaattgaggg gctttcttgc
    19021 cgtgattgtc aggagtcgac atggcatcct ccggttaagt tttttctcat taaccgaagg
    19081 atacactctg gagtcgactc cagagtcaag ttttatcaga gatacagcga gcggacgatg
    19141 aggaagtgac cggcaaagta gcaggccgca gcaatcgcgt tatccgcacg gaagcgtcgg
    19201 cgatagtggc tacccagcca gacaaagtta ctgataaaca gcaacgaagc gccgacaaac
    19261 gcagagagtg ccggagcggt cggacggaag aaccacagtt cacctgccag ccacaccatc
    19321 accagcgtca tgccgataaa ggtgcagata ggccaacgat actcttccag ccgcgtccag
    19381 ataatcgcca gtaacagcgc gcccagaacc agcagtacca acggcagagg ccagaagaaa
    19441 gagagcgtca tctgactggc gaaatagatg gtgtacagca ggtgcgagag gaaaaacgcg
    19501 ccgatggcgt acatcagacg ttgacgtggc aacagggtta gcgcatcgcc cagcagtgag
    19561 gcgcacagcc ccgccagcac cagatagcta atggcgtcga acatcggcgc ttgccaggcc
    19621 agtaacaaca ggagaagaag ggttaacggt ttaaacaccc agcgttgcca ggttggccca
    19681 cgatacgatg catccacaga tagccatgcg gaaagacaga cagcgataaa cgaccaaagc
    19741 atcttagttc cttgaattcg ttattttttc gtaagcggaa tgcctacggt cttgcttcca
    19801 gtttagtggc gagggtggac ggatgacaac accataactg gcagggtatg cttaattccg
    19861 cattttccga tgagggataa agatgagcaa accacctctt ttctttattg ttatcattgg
    19921 cttaatcatc gtcgccgcat cgtttcgttt tatgcaacag cgacgggaaa aagctgataa
    19981 tgatatggca ccgctccagc aaaagctggt ggtggtgagc aacaagcggg aaaaaccgat
    20041 taacgatcgc cgttcgcgcc agcaggaagt aactccggca ggcaccagta tgcgctatga
    20101 ggcaagcttc aaaccgcaaa gcggaggaat ggagcagacg tttcgcctcg acgcccagca
    20161 gtaccacgcc ctgacagtgg gcgataaagg tacgctgagc tataaaggaa cgcgctttgt
    20221 tagctttgtt ggcgaacaat aaggttattt tttaacttta aatttcttct gccagaccag
    20281 cagttcaaac acgccgaaaa ggaaaatccg caccttttcg gcggtggtca tctgcgggcc
    20341 atctttcggt aaagtggatt tcaatagcgc cagctgcata ccatgcatca gcacggtaaa
    20401 aatcagcgcc acgttaacga aaatattcag cgggcgcggg aagggatgca ccaggtttag
    20461 gattaaaaat ccccaaacgc agagcattaa caaacgtcca atattaatca gcatcacttt
    20521 ctccttgtgc ttcgcgttga tacagccgat aagccacctg acccgccact ttttcccgat
    20581 gcaatgacca gtttgccgga acagtgggca gaccgttttc gacttcgctt tcgacataaa
    20641 tcagggcttc gtcagccagc cagccgttat cttccagtaa atttatcgtc tcttctaaca
    20701 agccacggcg gaacggtgga tcgacaaaca cgatattatg cggtgtgcct ttttgcgcca
    20761 ggaatgacat cgcgttgctg ttcaccacgc gtgcattgcc tgcttttagt gtcgccagat
    20821 tcttaattaa ctgctgagaa actgcacgct ccatctcaat caacgtggcc cccgcagcgt
    20881 agcgcgataa cgcttccagc cccagcgcgc cgcttccggc gaagcaatcc agacattggg
    20941 cgtcaacaat gaccggagcc agccagttaa ataacgtttc gcgtacgcgg tcggtggtgg
    21001 ggcgcagacc tgggctatca ggaaccggga gtttacggcc tcgccactgc ccgccaataa
    21061 tgcggatttg gccgctgccg gaatgattcg gttttttcat gataaattgc tcaatccgcc
    21121 agataacaca taattgcagg cggtgatgtg aattaagtta agtgatagac tatttcatca
    21181 tttttttagc tgctatgtac atagcgttaa cgctgtgcca tgaagcaaca gcgaggagtg
    21241 tagtcgcaaa tggcgaaaga aaaaaaacgt ggcttttttt cctggctggg ctttggtcaa
    21301 aaagagcaga ccccggaaaa agagacagaa gttcagaatg aacaaccggt tgtagaagac
    21361 atcgttcagg cgcaagagcc tgtgaaggct tctgaacaag ccgttgaaga gcagccgcag
    21421 gcgcatactg aagccgaggc ggaaactttt gctgccgaag ttgtggaagt cactgaacag
    21481 gttgctgaaa gtgaaaaagc gcagcctgaa gcggaagtcg ttgcacagcc ggaaccggtc
    21541 gtagaagaaa cgccggagcc agtggctatc gaacgtgaag agctgccgtt gccggaagac
    21601 gtcaacgccg aagaggtttc gccagaagag tggcaggctg aagcggaaac cgtagagatt
    21661 gtcgaagcgg cggaagaaga agcggctaaa gaagaaatta ccgacgaaga gctggaaaca
    21721 caggcgctgg ctgccgaagc ggcagaagag gcggtgatgg tggttcctcc ggcagaagaa
    21781 gagcagccgg tggaagaaat cgctcaggag caggaaaaac cgaccaaaga aggttttttc
    21841 gcgcgcctga aacgcagcct gttaaaaacc aaagaaaatc tcggttccgg atttatcagc
    21901 ctgttccgcg gtaaaaaaat cgacgatgat ctgtttgaag agctggaaga acagctgttg
    21961 atcgccgatg tgggcgtgga aaccacacgt aaaattatca ccaatctgac agaaggcgca
    22021 tcccgcaagc agcttcgtga tgccgaggcg ctctatggcc tgctgaaaga agagatgggc
    22081 gagattctgg cgaaagtcga tgagccgctg aatgttgaag gtaaaacgcc attcgtgatc
    22141 ctgatggtgg gcgtcaacgg tgtgggtaaa accacgacga ttggtaagct ggcgcgtcag
    22201 tttgagcagc agggtaaatc ggtgatgctg gcggcgggcg atactttccg tgcagcagcg
    22261 gttgaacagc ttcaggtctg gggtcagcgc aacaatattc cggtgattgc ccagcatacc
    22321 ggtgcggatt ccgcctctgt tatcttcgac gccattcagg cagctaaagc gcgtaatatc
    22381 gacgtcttga ttgccgatac cgccggacgc ctgcagaaca aatcgcacct gatggaagag
    22441 ttgaagaaaa tcgtccgcgt gatgaagaaa ctcgacgttg aagcgccgca tgaagttatg
    22501 ctgactatcg atgccagcac cgggcagaac gcggtaagcc aggccaaact gttccacgaa
    22561 gccgttggct taaccggcat cacgctaacg aaactggacg gcacggcgaa aggcggggta
    22621 attttctcgg tggctgacca gtttggtatc cctatccgct acattggtgt cggcgaacgt
    22681 attgaggatt tgcgtccgtt taaggcggac gactttatag aggcactttt tgcccgagag
    22741 gattaacaat gattcgcttt gaacatgtca gcaaggctta tctcggtggg agacaggcgc
    22801 tgcagggcgt tacgttccat atgcagccgg gtgagatggc gtttctgacc ggtcattccg
    22861 gcgcagggaa aagtaccctc ctgaagctga tctgtgggat tgagcggccc agcgccggga
    22921 aaatctggtt tagcggccat gacatcacgc gtctgaaaaa ccgtgaagtt ccgtttctgc
    22981 gccgccagat tggcatgatt ttccaggatc accatctact gatggaccgc actgtctacg
    23041 ataacgtggc gatcccgctg attatcgctg gtgccagcgg tgacgatatt cgtcgccggg
    23101 tgtcggcggc actggataaa gtcgggctac tggacaaagc gaagaacttc cctattcagc
    23161 tttctggcgg tgaacaacag cgtgttggca ttgcccgcgc ggtggtgaat aagcccgcgg
    23221 tactgctggc ggacgaaccg actggtaact tggatgacgc gttgtcggaa gggattctac
    23281 gtctgtttga agagtttaac cgcgttgggg taaccgtatt gatggcaacg cacgacatca
    23341 acctgatttc acggcgttcc tatcgcatgc tcaccctgag cgatggtcac ttgcatggag
    23401 gcgtgggcca tgaataagcg cgatgcaatc aatcatattc ggcagtttgg cgggcgtctg
    23461 gatcgcttcc gtaaatcggt cggcggctca ggcgacggcg gtcgtaacgc gccgaaacgc
    23521 gcgaaatcct cgccaaaacc ggtaaatcgc aaaaccaacg ttttcaacga acaggtgcgc
    23581 tatgccttcc acggcgcatt gcaggatctg aaaagcaaac cgttcgccac gtttttaacg
    23641 gtgatggtta tcgccatctc tctgacgctg cccagcgtct gttatatggt gtacaaaaac
    23701 gttaaccagg cggcgacgca gtattatccg tcaccgcaaa tcactgttta tctgcaaaaa
    23761 acgctggacg atgacgctgc tgcgggcgtg gtggcacagt tgcaggccga gcaaggcgta
    23821 gagaaagtga attatctttc tcgtgaagac gcactgggcg agttccgtaa ctggtctggt
    23881 tttggtggtg cgctggatat gctggaagaa aacccgcttc cggcagtggc ggtggtgatc
    23941 ccgaaactcg atttccaggg tacggaatca ctcaatacgc tgcgtgatcg tatcacgcag
    24001 attaacggca ttgacgaagt acggatggat gacagctggt ttgcccgtct ggcggcgttg
    24061 accgggctgg tcgggcgcgt ttcggcgatg atcggcgtgt tgatggtggc ggcagtgttc
    24121 ctcgtcatcg gtaacagtgt acgcctgagt atctttgctc gtcgtgactc cattaacgtg
    24181 cagaaactga ttggtgcaac ggatggtttc atcctgcgcc cgttcctgta cggtggcgca
    24241 ttgctggggt tttctggcgc attgttgtca ctaattttgt cagaaattct ggtactgcga
    24301 ttgtcatcgg cggttgcgga agtggcacag gttttcggaa cgaagtttga tatcaatggc
    24361 ttatcgttcg atgaatgcct gctattgctg ctggtatgct cgatgattgg ctgggtggca
    24421 gcgtggcttg ccacggtaca acatttacgc cactttacgc ctgaataata aaagcgtgtt
    24481 atactctttc cctgcaatgg gttccgtagc agggaaagag accccgttgt ctcttcccgg
    24541 tatttcatct ctatgtcaca ttttgtgcgt aatttattca caagcttgca ttgaacttgt
    24601 ggataaaatc acggtctgat aaaacagtga atgataacct cgttgctctt aagctctggc
    24661 acagttgttg ctaccactga agcgccagaa gatatcgatt gagaggattt gaatgactga
    24721 caaaatgcaa agtttagctt tagccccagt tggcaacctg gattcctaca tccgggcagc
    24781 taacgcgtgg ccgatgttgt cggctgacga ggagcgggcg ctggctgaaa agctgcatta
    24841 ccatggcgat ctggaagcag ctaaaacgct gatcctgtct cacctgcggt ttgttgttca
    24901 tattgctcgt aattatgcgg gctatggcct gccacaggcg gatttgattc aggaaggtaa
    24961 catcggcctg atgaaagcag tgcgccgttt caacccggaa gtgggtgtgc gcctggtctc
    25021 cttcgccgtt cactggatca aagcagagat ccacgaatac gttctgcgta actggcgtat
    25081 cgtcaaagtt gcgaccacca aagcgcagcg caaactgttc ttcaacctgc gtaaaaccaa
    25141 gcagcgtctg ggctggttta accaggatga agtcgaaatg gtggcccgtg aactgggcgt
    25201 aaccagcaaa gacgtacgtg agatggaatc acgtatggcg gcacaggaca tgacctttga
    25261 cctgtcttcc gacgacgatt ccgacagcca accgatggca ccggtgctct atctgcagga
    25321 taaatcatct aactttgccg acggcattga agatgataac tgggaagagc aggcagcaaa
    25381 ccgtctgacc gacgcgatgc agggtctgga cgagcgtagc caggatatca tccgcgcgcg
    25441 ctggctggac gaagacaata agtccacgtt gcaggaactg gctgaccgtt acggtgtttc
    25501 cgctgaacgt gtacgccagc tggaaaagaa cgcgatgaaa aaactgcgcg ctgctataga
    25561 agcgtaattt ccgctattaa gcagagaacc ctggatgcga gtccggggtt tttgtttttt
    25621 gggcctctac aataatcaat ttcccctccg gcaaaacgcc aatccccacg cagattgtta
    25681 ataaactgtc aaaatagcta ttccaatatc ataaaaatcg ggtatgtttt agcagagtat
    25741 gctgctaaag cacgggtagt catgcataaa acgaaataaa gtgctgaaca acaacatcac
    25801 aacacacgta ataaccagaa gaatggggat tctcaggatg aacataaagg gtaaagcgtt
    25861 actggcagga tgtatcgcgc tggcattcag caatatggct ctggcagaag atattaaagt
    25921 cgcggtcgtg ggcgcaatgt ccggtccggt tgcgcagtac ggtgaccagg agtttaccgg
    25981 cgcagagcag gcggttgcgg atatcaacgc taaaggcggc attaaaggca acaaactgca
    26041 aatcgtaaaa tatgacgatg cctgtgaccc gaaacaggcg gttgcggtgg cgaacaaagt
    26101 cgttaacgac ggcattaaat acgtgattgg tcacctctgt tcttcatcaa cgcagcctgc
    26161 gtctgacatc tacgaagacg aaggcatttt aatgatcacc ccagcggcaa ccgcgccgga
    26221 gctgaccgcc cgtggctatc agctgatcct gcgcaccacc ggcctggact ccgaccaggg
    26281 gccgacggcg gcgaaatata ttcttgagaa agtgaaaccg cagcgtattg ctatcgttca
    26341 cgacaaacag caatacggcg aaggtctggc gcgagcggtg caggacggcc tgaagaaagg
    26401 caatgctaac gtggtgttct ttgatggtat caccgccggg gaaaaagatt tctcaacgct
    26461 ggtggcgcgt ctgaaaaaag agaatatcga cttcgtttac tacggcggtt atcacccgga
    26521 aatggggcaa atcctgcgtc aggcacgcgc ggcagggctg aaaactcagt ttatggggcc
    26581 ggaaggtgtg gctaacgttt cgctgtctaa cattgcgggc gaatcagcgg aagggctgct
    26641 ggtgaccaag ccgaagaact acgatcaggt tccggcgaac aaacccattg ttgatgcgat
    26701 caaagcgaaa aaacaggacc caagcggcgc attcgtctgg accacctacg ccgcgctgca
    26761 atctttgcag gcgggcctga atcagtctga cgatccggct gaaatcgcca aatacctgaa
    26821 agcgaactcg gttgataccg tgatggggcc gctgacctgg gatgagaaag gcgatctgaa
    26881 aggctttgag ttcggcgtat ttgactggca cgccaacggc acggcaaccg atgcgaagta
    26941 acagatagat aaatgaaaat gcccgatgcg ctgaactgcc atcgggtttt tttgtgtttg
    27001 tcgaaatggt tgtagaaaga ttaaacaatc caggatatgc ttaatgtacg gtgacatacc
    27061 gtacatatta tacggagcag gagagttcac atgtctgctg ttaaaaagca gcgtatcgat
    27121 ctgcgtttaa ctgacgatga caaaagtatg attgaggaag cggcagcgat atctaatcag
    27181 tccgtcagcc aatttatgtt gaacagcgct tcgcagcggg ctgcggaagt gattgaacag
    27241 catcggcggg tgatcctcaa tgaggaatcc tggacgcggg tgatggatgc gctgagtaat
    27301 ccaccgtcac ctggtgaaaa gctaaaacgt gcggcaaaac gtcttcaggg aatgtaataa
    27361 tttatggatg atctgacgat agagattctg accgatgatg cagattatga tctacagcga
    27421 ttcgactgcg gcgaggaagc gttaaatctc tttctgacga cacatctggt tcgtcaacat
    27481 cgcaacaaaa ttctgcgagc gtatatcctt tgtcgcaaca ctccagaacg tcaggtgctg
    27541 ggatattaca cattatccgg cagttgtttt gaacgagccg cattgccctc gaaatcgaaa
    27601 cagaaaaaaa ttccctacaa aaatattccc agcgttactc ttgggcgtct ggcaattgat
    27661 cgttcattac aggggcaggg atggggagca acactggttg ctcatgccat gaaagtcgtc
    27721 tggtcagcct ctttagcggt aggtattcac ggtctttttg tcgaggcgct gaatgaaaaa
    27781 gcccatacgt tttataaatc gctgggcttt atccctttag tcggggaaaa cgaaaatgcg
    27841 ttatttttcc caaccaaatc cattgaactg ctttttacac agagcgatta acgcttctcc
    27901 cagccgccct gctgtgccgt aaaccccagc gcctgcataa acgccgtcat cacaccgcga
    27961 tcttccacgc ctgcatccgc catccaccag catgaaacgc caggattgtt acgcaaaacc
    28021 tcttccagca gatattgccc cacaccgcga cgtcgagtga cttcccgcac gcgcagggaa
    28081 tccagtgttc cctctgtgcc gcttaaggtt acccgtacgg cagcgagcag gcgctcgtta
    28141 aaacgcgcgg cgtagatacg gtggttatcg tcaacctgta acgaggaagg ggaatactcc
    28201 ggccagatct tttgcaggtc aatccggtcc tggtcgctaa agttttctaa tcgaatgatg
    28261 gtcagcttca tggggaaccc gtgtaaatca caaaagtgga accagtgtag cgaaataatt
    28321 taatcggagg ctttctcttt tttatttctt ttggcaggtg attaattttt taacagcaat
    28381 aattacaaag ttaaaacatt agagaatgaa aaatacccag cataatcccc tgaatggtag
    28441 tgaattattc cgccctttgt gccgttattt tatgctgaca aaggcacttt tttctgttta
    28501 tctatcaata aattcagaat attatctgtt cttaatcgac tgaaaaatag ggactttaat
    28561 cgctattatc acaaaatact gcgctaaacc cttaatcaga caggcaaaaa cagtgcagta
    28621 taaaaaaaga acagtctgat ttgttaacac ataaaaacaa agcaacacaa catcacgaat
    28681 ggggattttt gactatgaaa cggaatgcga aaactatcat cgcagggatg attgcactgg
    28741 caatttcaca caccgcttta gccgacgata ttaaagttgc cgttgtcggc gcgatgtccg
    28801 gcccgattgc ccagtggggc gatatggaat ttaacggcgc gcgtcaggcg attaaagaca
    28861 tcaatgccaa agggggaatt aaaggcgata agctggttgg cgtggaatat gacgacgcct
    28921 gcgacccgaa acaagccgtt gcggtcgcca acaaaatcgt taatgacggc attaaatacg
    28981 ttattggtca tctgtgttct tcttctaccc agcctgcgtc agatatctat gaagatgaag
    29041 gtatcctgat gatctcgccg ggggcgacca acccggagct gacccaacgc ggttatcaac
    29101 acatcatgcg tactgccggg ctggactctt cccaggggcc aacggcggca aaatacattc
    29161 ttgagacagt gaagccccag cgcatcgcca tcattcacga caaacaacag tatggcgaag
    29221 ggctggcgcg ttcggtgcag gacgggctga aagcggctaa cgccaacgtc gtcttcttcg
    29281 acggcattac cgccggggag aaagatttct ccgcgctgat cgcacgcctg aaaaaagaaa
    29341 acatcgactt cgtttactac ggcggttact acccggaaat ggggcagatg ctgcgccagg
    29401 cccgttccgt cggcctgaaa actcagttta tggggccgga aggcgtaggt aacgcatcat
    29461 tgtcgaatat tgccggtgat gccgccgaag gcatgttggt cactatgcca aaacgctatg
    29521 accaggatcc ggcaaaccag ggcatcgttg atgcgctgaa agcagacaag aaagatccgt
    29581 ccgggcctta tgtctggatc acctacgcgg cggtgcaatc tctggcaact gcccttgagc
    29641 gtaccggcag cgatgagccg ctggcgctgg tgaaagattt aaaagctaac ggtgcaaaca
    29701 ccgtgattgg gccgctgaac tgggatgaaa aaggcgatct taagggattt gattttggtg
    29761 tcttccagtg gcacgccgat ggttcatcca cggcagccaa gtgatcatcc caccgcccgt
    29821 gaaatgcggg cgggtttaga aaggttacct tatgtctgag cagtttttgt atttcttgca
    29881 gcagatgttt aacggcgtca cgctgggcag tacctacgcg ctgatagcca tcggctacac
    29941 catggtttac ggcattatcg gcatgatcaa cttcgcccac ggcgaggttt acatgatcgg
    30001 cagctacgtc tcatttatga tcatcgccgc actaatgatg atgggcattg ataccggctg
    30061 gctgctggtg gcggcgggat tcgtcggcgc aatcgtcatt gccagcgcct acggctggag
    30121 tatcgaacgg gtagcctatc gcccggtgcg taactctaag cgcctgattg cgctcatctc
    30181 ggcaatcggc atgtccatct tcctgcaaaa ctacgtcagc ctgaccgaag gttcgcgcga
    30241 cgtagcactg ccgagcctgt ttaacggtca gtgggtggta ggccatagcg aaaacttctc
    30301 tgcctctatt accactatgc aggcggtgat ctggattgtt accttcctcg ccatgctggc
    30361 gctgacgatt ttcattcgct attcccgcat gggtcgcgcc tgtcgtgcct gcgccgaaga
    30421 tctgaaaatg gcgagcctgc ttggcatcaa caccgaccgg gtgattgcgc tgacctttgt
    30481 gattggcgcg gcaatggcgg cggtggcggg cgtactgctc ggtcagttct acggcgtcat
    30541 taacccctat atcggcttta tggccgggat gaaagccttt accgctgcgg tgctcggtgg
    30601 gattggcagc attccgggag cgatgattgg cgggctgatt ctggggattg cggaggcgct
    30661 ctcttctgcc tatctgagta cggaatataa agatgtggtc tcattcgccc tgctgattct
    30721 ggtgctgctg gtgatgccga ccggtattct gggtcgcccg gaggtagaga aagtatgaaa
    30781 ccgatgcata ttgcaatggc aatgctctct gccgcgatgt tctttgtcct cgctggcgtc
    30841 tttatgggcg tgcaactgga gctggatggc accaaactgg tggtcgatag cgcttcggat
    30901 gtccgttggc agtgggtgtt tatcggcacg gcggtggtct ttttcttcca gcttttgcga
    30961 ccggctttcc agaaagggtt gaaaagcgtt tccggaccga agtttattct gcccgcgatt
    31021 gatggctcca cggtgaagca gaaactgttc ctcgtggcgc tgttggtgct tgcggtggcg
    31081 tggccgttta tggtttcacg cgggacggtg gatattgcca ccctgaccat gatctacatt
    31141 attctcggcc tcgggctgaa cgtggtggtg gggttatccg gcctgctggt gctcggttac
    31201 ggcggctttt acgccatcgg cgcttacact tttgcgctgc tcaatcacta ttacggtttg
    31261 ggcttctgga cctgtctgcc gattgccggg ttaatggcgg cggcggcggg cttcctgctt
    31321 ggttttccgg tgctgcgtct gcgcggtgat tatctggcga tcgttaccct cggtttcggc
    31381 gaaattgtgc gcatattgct gctcaataac accgaaatta ccggcggccc gaacggaatc
    31441 agtcagatcc cgaaaccgac actcttcgga ctcgagttca gccgttccgc tcgtgaaggc
    31501 ggctgggaca cgttcagtaa tttctttggt ctgaaatacg atccctccga tcgtgtcatc
    31561 ttcctctacc tggtggcgtt gctgctggtg gtgctaagcc tgtttgtcat taaccgcctg
    31621 ctgcggatgc cgctggggcg tgcgtgggaa gcgttgcgtg aagatgaaat cgcctgccgt
    31681 tcgctgggct taagcccgcg tcgtatcaag ctgactgcct ttaccatcag tgccgcgttt
    31741 gccggttttg ccggaacgct gtttgctgcg cgccagggct ttgtcagccc ggaatccttc
    31801 acctttgctg aatcagcctt tgtactggcg atagtggtgc tcggcggtat gggctcgcaa
    31861 tttgcagtga ttctggcggc tgttctgctg gtggtatcgc gcgagttgat gcgtgatttc
    31921 aacgaataca gcatgttaat gctcggtggt ttgatggtgc tgatgatgat ctggcgtccg
    31981 cagggcttgc tgcccatgac gcgcccgcaa ctgaagctga aaaacggcgc agcgaaagga
    32041 gagcaggcat gagtcagcca ttattatctg ttaacggcct gatgatgcgc ttcggcggcc
    32101 tgctggcggt gaacaacgtc aatcttgaac tgtacccgca ggagatcgtc tcgttaatcg
    32161 gccctaacgg tgccggaaaa accacggttt ttaactgtct gaccggattc tacaaaccca
    32221 ctggcggcac tattttactg cgtgatcagc atctggaagg tttgccgggg cagcaaatcg
    32281 cccgcatggg cgtggtgcgt accttccagc atgtgcgtct gttccgtgaa atgacggtaa
    32341 ttgaaaacct gctggtggcg cagcatcagc aactgaaaac cgggctgttc tctggcctgt
    32401 tgaaaacgcc ttctttccgt cgcgcccaga gcgaagcgct cgaccgcgcc gccacctggc
    32461 ttgagcgcat tggtttgctg gaacacgcca accgtcaggc gagtaacctg gcctatggtg
    32521 accagcgccg tcttgagatc gcccgctgca tggtgacgca gccggagatt ttaatgctcg
    32581 acgaacctgc ggcaggtctt aacccgaaag aaacgaaaga gctggatgag ctgattgccg
    32641 aactgcgcaa tcatcacaac accactatct tgttgattga acacgatatg aagctggtga
    32701 tgggaatttc ggaccgaatt tacgtggtca atcaggggac gccgctggca aacggtacgc
    32761 cggagcaaat ccgtaataac ccggatgtga tccgtgccta tttaggtgag gcataagatg
    32821 gaaaaagtca tgttgtcctt tgacaaagtc agcgcccact acggcaaaat ccaggcgctg
    32881 catgaggtga gcctgcatat caatcagggc gagattgtca cgctgattgg cgcgaacggg
    32941 gcggggaaaa ccaccttgct cggcacgtta tgcggcgatc cgcgcgccac cagcgggcga
    33001 attgtgtttg atgataaaga cattaccgac tggcagacgg cgaaaatcat gcgcgaagcg
    33061 gtagcgattg tccccgaagg gcgtcgtgtc ttctcgcgga tgacggtgga agagaacctg
    33121 gcgatgggcg gcttttttgc tgaacgcgac cagttccagg agcgcataaa gtgggtctat
    33181 gaactgtttc cacgtctgca tgagcgccgt gttcagcggg caggcaccat gtccggcggt
    33241 gagcagcaga tgctggcgat tggtcgcgct ctgatgagca acccgcgttt gttgttgctt
    33301 gatgagccat cgctcggtct tgcgccgatt atcatccagc aaattttcga caccatcgag
    33361 cagctgcgcg agcaggggat gactatcttc ctcgtcgagc agaacgccaa ccaggcgcta
    33421 aagctggcgg atcgcggcta cgtgctggaa aacggtcatg tggtgctctc cgatactggt
    33481 gatgcactac tggcgaacga agcggtgaga agtgcgtatt taggcgggta ataacacgtt
    33541 gattgataag gagtcaaaag actcctttga gacaggtgac aaatgtaaaa ttgcctgatg
    33601 cgctgcgctt atcaggccta ctgggtgagt tgcaatatat tgaatttgca cgatcttgta
    33661 ggccggataa ggcgtttacg ccgcatccgg catgaaacga tgagtaatct gtagagtttg
    33721 attcagacct tctatttttc cgcttatccg tgccccatct cccattttcc ctcacccgta
    33781 ccgtcgccgc cttgtcatct ttctgacacc ttactatctt acaaatgtaa caaaaaagtt
    33841 atttttctgt aattcgagca tgtcatgtta ccccgcgagc ataaaacgcg tgaattcgcg
    33901 cattcggtac aacaagagag ataaacgatg aaaccgttac attatacagc ttcagcactg
    33961 gcgctcggac tggcgttaat ggggaatgca caggcagtga cgaccattcc gttctggcat
    34021 tctatggaag gggaactggg taaagaggtg gattctctgg cccaacgttt taacgccgaa
    34081 aacccggatt acaaaattgt accgacctat aaaggcaact acgaacagaa tttaagcgcg
    34141 gggattgccg catttcgtac cggcaatgct ccggctattt tgcaggttta tgaagttggc
    34201 accgccacca tgatggcatc gaaagccatt aaaccggtat atgacgtgtt taaagaggcg
    34261 gggattcaat tcgatgagtc gcagtttgtg ccgacggttt ccggttacta ctccgacagc
    34321 aaaacgggcc acttactctc ccagccattc aacagctcga cccccgttct ctattacaac
    34381 aaagacgcct tcaagaaagc aggattagac ccggaacaac cgccgaaaac ctggcaggat
    34441 ctggcggact atgccgcaaa actaaaagcc tccggcatga agtgcggcta cgccagcggc
    34501 tggcagggct ggatccaact ggaaaacttt agcgcctgga acggtctgcc gtttgccagc
    34561 aaaaacaacg gttttgacgg cacagacgcg gtgctggagt tcaacaagcc ggagcaggtg
    34621 aaacacatcg ccatgctcga agagatgaac aagaagggcg atttcagcta cgtcgggcgt
    34681 aaggatgaat ctaccgagaa gttctataac ggtgattgtg cgatgaccac cgcctcttcc
    34741 ggttctcttg ccaacattcg cgagtacgcc aaatttaact acggcgtagg catgatgcct
    34801 tacgacgccg atgcgaaaga tgcgccacaa aacgccatta tcggcggagc cagcctgtgg
    34861 gtgatgcagg gcaaaaataa agaaacgtat accggcgtgg cgaagttcct cgacttcctc
    34921 gcgaagccag aaaacgctgc cgagtggcat cagaaaaccg gttatctgcc aatcaccaaa
    34981 gcagcgtatg acctgacccg tgagcagggc ttttatgaga aaaacccagg ggcggatacc
    35041 gcgacgcgtc agatgctgaa taagccgccg ttgccgttca ccaaagggct gcgtctgggc
    35101 aacatgccgc agatccgcgt gattgtggat gaagagctgg agagcgtgtg gaccggtaag
    35161 aagacaccac agcaggcact ggataccgcc gttgagcgtg gaaatcagtt gctgcgccgc
    35221 tttgagaaat cgactaagtc ttaatcagtg taatgtcgga tgcgtttcgc ttatctgacc
    35281 tggcatcgcg tgtaggccgg ataagcgaag cgcatccggc acagttcagg aattaaccgt
    35341 aatgtcatca tcccgtccgg tgttccgctc gcgctggctg ccttatctgc tggtcgcgcc
    35401 gcagctcatc atcaccgtta tcttttttat ctggcctgcg ggcgaagcgt tgtggtactc
    35461 gctacaaagc gtcgatccgt ttggtttctc cagccagttt gtcggcctgg ataacttcgt
    35521 cacgctgttc catgacagct actatctcga ctccttctgg acgacgataa aattcagcac
    35581 ctttgtcacc gtcagcggtt tgctggtatc gctgttcttt gcggcgctgg tggagtacat
    35641 cgtgcgcggc agccgtttct atcaaacctt aatgttgctg ccttacgccg tggctcccgc
    35701 cgttgccgcc gtattgtgga tcttcctgtt taaccccggt cgcgggctga tcacccattt
    35761 tctcgctgag ttcggctacg actggaacca cgcgcaaaac agcggtcagg caatgtttct
    35821 ggtggtgttt gcctcagtat ggaagcaaat cagctacaac ttcctgttct tctatgccgc
    35881 gctgcaatcc attccccgtt cgttgatcga agccgcagcc atcgacggtg cagggccgat
    35941 tcgccgcttc tttaagattg cgctgccgct tatcgccccg gtgagtttct tcctgctggt
    36001 agtgaacctg gtgtatgcct tcttcgacac cttcccggtg atcgacgccg ccacgtccgg
    36061 tggaccagtg caggctacca cgacgctgat ttataagatc taccgcgaag gctttaccgg
    36121 actggatctg gcttcgtctg ccgcgcagtc ggtggtgttg atgttcctcg tcatcgtgct
    36181 gacggtggtg cagttccgct atgttgaaag caaggtgcgt taccaatgat tgagaaccgt
    36241 ccgtggctga cgatattcag ccataccatg ctgatcctcg ggatcgcggt gatcctattc
    36301 ccgctgtacg tggcgtttgt cgcggcgacg ctggataaac aggccgtcta cgccgcgccg
    36361 atgacgctca tccccggcac gcatctgctg gaaaacatcc acaacatctg ggtaaacggg
    36421 gtaggcacaa atagtgcgcc gttctggcgg atgttgctta acagcttcgt gatggcgttc
    36481 agcattacgc tcggcaaaat taccgtctcg atgctctcgg cattcgccat tgtctggttt
    36541 cgttttccgc tgcgtaacct ctttttctgg atgattttta tcaccctgat gttgccggtt
    36601 gaagtacgta tcttcccgac ggtggaagtc atcgccaacc tgaagatgct cgacagctac
    36661 gccggtttaa cgctgccgct gatggcctcg gcgaccgcga cgtttctttt ccgccagttc
    36721 tttatgaccc taccggatga actggtggaa gccgcgcgga tcgacggcgc atcgccaatg
    36781 cgcttctttt gcgacatcgt ttttccgttg tccaaaacta atctggcggc gttgtttgtg
    36841 atcaccttta tttacggctg gaaccagtat ttgtggccgt tgttgattat taccgacgtg
    36901 gatctcggca ccaccgtggc agggatcaaa gggatgatcg ccactggcga aggcaccacg
    36961 gaatggaact cggtgatggc tgctatgttg ttaacgctta tccctccggt ggtgattgtt
    37021 ttagtgatgc agcgtgcctt tgtgcgcggc ctggtcgata gtgagaaata agatggcagg
    37081 actgaaatta caggcagtaa ccaaaagctg ggatggcaaa acccaggtga ttaaaccact
    37141 gacccttgat gtggcggatg gcgaatttat cgtgatggtc gggccgtctg gctgcggaaa
    37201 atcgacgctg ctgcgcatgg ttgccgggct ggagcgggtg acggaaggcg atatctggat
    37261 caacgaccag cgcgtgaccg aaatggagcc gaaagatcgc gggattgcga tggtgttcca
    37321 gaactacgcg ctttatccgc atatgagcgt cgaagaaaac atggcgtggg ggctgaaaat
    37381 tcgcggcatg ggcaagcagc aaatagccga gcgcgttaaa gaagcggcgc gcattctgga
    37441 gctggatggc ctgcttaagc gccgcccgcg cgagctatcc ggcggtcagc gtcagcgtgt
    37501 ggcgatgggt cgcgcgattg tgcgcgatcc ggcggtgttc ctgtttgatg agccgctctc
    37561 taacctcgat gccaagctgc gcgtgcagat gcgccttgag ctgcaacagc tgcaccgtcg
    37621 cctgaaaacg acttcactct acgttactca cgatcaggtt gaagcgatga cgctcgccca
    37681 gcgagtaatg gtgatgaacg gcggcgttgc cgaacagatt ggcacgccag ttgaagtgta
    37741 cgaaaagccc gccagcctgt ttgtggcgag ttttatcggc agcccggcga tgaacctgct
    37801 gacaggccgc gtgaataatg aaggcacgca tttcgaactg gacggcggta tcgtgctacc
    37861 gctaaacggt ggctaccgtc agtatgccgg gcgtaaaatg actctcggca ttcgcccgga
    37921 acatatagcg ctaagctcgc aggcagaagg tggtgtgccg ctggtgatgg acacgctgga
    37981 gatcctcggc gcagataacc tggcgcacgg acgctggggc gagcagaagc tggtggtgcg
    38041 actggcgcat caggagcgcc cgacggcagg cagcacgctg tggctgcatc tggcggaaaa
    38101 tcagctgcat ctttttgatg gtgaaacagg acaaagagta tgagtaactg gccttatccc
    38161 cgcatcgtcg ctcatcgtgg cggcggtaag ctggccccgg aaaacaccct ggcggcaatc
    38221 gatgtcgggg caaaatacgg tcataagatg atcgaatttg acgcgaagtt atcgaaagat
    38281 ggcgagatct tcctgctcca tgacgacaat ctcgaacgca ccagcaacgg ctggggcgtc
    38341 gcgggtgaac tgaactggca ggatttacta cgcgtggatg cgggcagttg gtacggcaaa
    38401 gcgtttaaag gtgagccgct gccgttgctt tcgcaggtgg cggaacgctg tcgcgaacat
    38461 gggatgatgg cgaatatcga aatcaaaccc accactggca ctgggccatt aacgggcaaa
    38521 atggtggcgc tggcggcacg cgagctgtgg gccggtatga cgccgccgct actgtcatcg
    38581 tttgagattg atgctttaga agctgcacaa caggcggcac cggaactgcc gcgtggttta
    38641 ttgcttgatg agtggcgcga cgactggcgc gaactgaccg cgcggctggg ctgcgtctct
    38701 attcatctca atcataagtt actcgataaa gcgcgagtga tgcagttgaa agacgccgga
    38761 ttacggattc tggtttatac cgtcaataaa ccccagcgcg cggcagagtt gctgcgctgg
    38821 ggcgtggatt gcatctgtac cgatgcgatt gacgtgattg gtccgaactt tacggcccaa
    38881 tagttttcaa cggaatgtca ggctgcggcg tgctgggctg attcagcatg tcgccgtttc
    38941 tcctctccgg cagcatatgt tgctgattca acgaactatc cggattacgg ttgctgttta
    39001 acatcccgcc gttggtgttg ggcagcattt gctgccgggc gggatttcgc tcccccggct
    39061 gtgactgcaa cacccgctga gaattattgt ttatctggtt ttctaaatgc tgctgttgca
    39121 actgagtttg cgttttcagt tgctgattca gcatcccttt ttgctggatt tgctgagtct
    39181 gcatttgggt ttgcatccgc tgctggctgg gtatctgata ccccggctgg ttagggttgt
    39241 tcagagtatt aatgggctgt gcaaagccga caaacggcag gagtgccgta agaatcagaa
    39301 gtcgtttcat cgcgtatcct cctctgaaga tatcctttaa gtttactcgc ttcccgacaa
    39361 aacgatgatt aattcagagt tatataccag gcttagctgg ggttgcccct taatctctgg
    39421 agaataacga tgataaaacc gacgttttta cgccgggtgg ccattgctgc tctgctctta
    39481 ggaagttgtt ttagcgccgc cgccgcgcct cctgcgccgc ccgtctcgta tggtgtggag
    39541 gaagatgtct tccacccggt acgcgcgaaa cagggaatgg tagcgtctgt ggacgccact
    39601 gccactcagg tgggggtgga tattctcaag gagggcggga atgccgttga tgccgccgtg
    39661 gcggtgggct acgcgctggc ggtaacgcat ccgcaggcag ggaatctggg cggtggtggt
    39721 tttatgttaa tccgctcgaa aaatggcaat accacggcta tcgatttccg cgaaatggca
    39781 cccgccaaag cgacccgcga tatgttcctc gatgatcagg gcaacccgga cagcaaaaaa
    39841 tcactcactt cgcatctggc ttccggcaca ccgggtacgg tagcaggttt ctcgctggcg
    39901 ctggataaat acggcaccat gccgctgaac aaagtcgtgc agcccgcgtt taaactggca
    39961 cgcgatggtt ttatcgttaa cgacgcgctg gctgacgatc tcaaaaccta cggtagcgaa
    40021 gtgttgccga atcacgaaaa cagtaaagct atcttctgga aagagggcga gccgctgaaa
    40081 aagggcgaca cgctggtgca ggcgaacctg gcaaagagcc tggagatgat tgccgaaaac
    40141 ggcccggacg aattctataa aggcacgata gcggaacaga ttgcccagga gatgcagaaa
    40201 aacggtggct tgatcactaa agaagattta gcggcgtata aagcggtcga acgcacaccg
    40261 ataagtggcg attatcgcgg gtatcaggtt tactccatgc caccgccatc ctccggcggg
    40321 atccatatcg tacaaatcct caatattctg gaaaacttcg atatgaagaa atacggcttt
    40381 ggcagcgccg atgcgatgca aatcatggca gaagcggaga aatacgccta cgccgaccgc
    40441 tcggaatatc ttggcgaccc ggattttgtc aaagtaccgt ggcaggcgct gaccaataaa
    40501 gcctatgcca aatctattgc cgatcaaatt gatatcaata aagcgaagcc atccagcgaa
    40561 attcgccccg gcaagcttgc gccttatgag agtaatcaaa ctacccatta ctcagtggtg
    40621 gataaagatg gtaacgcggt ggcggtgacc tatacgctga acaccacctt cggtacgggc
    40681 attgtcgcgg gcgagagcgg tattctgctt aataatcaga tggatgattt ctccgctaaa
    40741 ccgggcgtac cgaacgttta cgggctggtg ggcggtgatg ccaacgccgt cgggccgaat
    40801 aaacgtccgc tgtcgtcaat gtcaccaacc attgtggtga aagacggtaa aacctggctg
    40861 gtcaccggta gccctggcgg gagccggatc atcactacag tgctgcaaat ggtggtgaat
    40921 agcatcgatt atggcatgaa cgtcgccgaa gcgaccaatg cgccgcgttt ccaccatcag
    40981 tggttgccgg acgagctgcg tgtcgaaaaa gggtttagcc cggatacgct caagctgctg
    41041 gaagcaaaag gtcagaaagt ggcgctgaaa gaggcgatgg gcagtacaca aagcattatg
    41101 gttgggccgg acggtgagtt gtacggcgca tccgacccgc gctcggtgga tgatttaacg
    41161 gcggggtact aaggttagcg gccctcttcg tgggaagagg gctattttgt cagggcaagc
    41221 cgaaggtagc cttttttatt tcgtaatcct gtagatattc ttccagcggc tttgccgttc
    41281 ccagagaatg aatttcacca ctgtctttat caataataaa aggtgcgtta ccagctaagc
    41341 gcgcggcctc atctccagtt tcgagaaatt ctcgtgcttc gaaacagaaa taccagccct
    41401 ggctaaagcg tccatgtaga gtaatgacga ccgggagatc tgcatcatca aggtaat
//
LOCUS       DAPGOF010000018        41079 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.018, whole genome shotgun sequence.
ACCESSION   DAPGOF010000018 DAPGOF010000000
VERSION     DAPGOF010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41079
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.018"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            337..1626
                     /gene="sdaC"
                     /locus_tag="RHV13_000867"
     CDS             337..1626
                     /gene="sdaC"
                     /locus_tag="RHV13_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HDV1240423.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1684..3051
                     /gene="sdaB"
                     /locus_tag="RHV13_000868"
     CDS             1684..3051
                     /gene="sdaB"
                     /locus_tag="RHV13_000868"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HDV1240424.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3163..3918
                     /gene="xni"
                     /locus_tag="RHV13_000869"
     CDS             3163..3918
                     /gene="xni"
                     /locus_tag="RHV13_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HDV1240425.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(3973..5121)
                     /gene="fucO"
                     /locus_tag="RHV13_000870"
     CDS             complement(3973..5121)
                     /gene="fucO"
                     /locus_tag="RHV13_000870"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HDV1240426.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVIDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGEKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5149..5796)
                     /gene="fucA"
                     /locus_tag="RHV13_000871"
     CDS             complement(5149..5796)
                     /gene="fucA"
                     /locus_tag="RHV13_000871"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HDV1240427.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6342..7658
                     /gene="fucP"
                     /locus_tag="RHV13_000872"
     CDS             6342..7658
                     /gene="fucP"
                     /locus_tag="RHV13_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HDV1240428.1"
                     /translation="MGNTSIQTQSYRAVDKDAGKSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            7691..9466
                     /gene="fucI"
                     /locus_tag="RHV13_000873"
     CDS             7691..9466
                     /gene="fucI"
                     /locus_tag="RHV13_000873"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HDV1240429.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            9575..10993
                     /gene="fucK"
                     /locus_tag="RHV13_000874"
     CDS             9575..10993
                     /gene="fucK"
                     /locus_tag="RHV13_000874"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HDV1240430.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIERLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            10995..11417
                     /gene="fucU"
                     /locus_tag="RHV13_000875"
     CDS             10995..11417
                     /gene="fucU"
                     /locus_tag="RHV13_000875"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HDV1240431.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVEQRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            11475..12206
                     /gene="fucR"
                     /locus_tag="RHV13_000876"
     CDS             11475..12206
                     /gene="fucR"
                     /locus_tag="RHV13_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HDV1240432.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(12250..13350)
                     /gene="rlmM"
                     /locus_tag="RHV13_000877"
     CDS             complement(12250..13350)
                     /gene="rlmM"
                     /locus_tag="RHV13_000877"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HDV1240433.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(13343..13738)
                     /locus_tag="RHV13_000878"
     CDS             complement(13343..13738)
                     /locus_tag="RHV13_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HDV1240434.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13757..14674)
                     /gene="gcvA"
                     /locus_tag="RHV13_000879"
     CDS             complement(13757..14674)
                     /gene="gcvA"
                     /locus_tag="RHV13_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HDV1240435.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(15025..15252)
                     /locus_tag="RHV13_000880"
     CDS             complement(15025..15252)
                     /locus_tag="RHV13_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HDV1240436.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            15444..16649
                     /gene="csdA"
                     /locus_tag="RHV13_000881"
     CDS             15444..16649
                     /gene="csdA"
                     /locus_tag="RHV13_000881"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HDV1240437.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            16649..17092
                     /gene="csdE"
                     /locus_tag="RHV13_000882"
     CDS             16649..17092
                     /gene="csdE"
                     /locus_tag="RHV13_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HDV1240438.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(17143..17949)
                     /gene="tcdA"
                     /locus_tag="RHV13_000883"
     CDS             complement(17143..17949)
                     /gene="tcdA"
                     /locus_tag="RHV13_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HDV1240439.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRSKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(18188..19285)
                     /gene="mltA"
                     /locus_tag="RHV13_000884"
     CDS             complement(18188..19285)
                     /gene="mltA"
                     /locus_tag="RHV13_000884"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HDV1240440.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            19494..19570
                     /locus_tag="RHV13_000885"
     tRNA            19494..19570
                     /locus_tag="RHV13_000885"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19528..19530,aa:Met,seq:cat)
     gene            complement(19644..20897)
                     /gene="amiC"
                     /locus_tag="RHV13_000886"
     CDS             complement(19644..20897)
                     /gene="amiC"
                     /locus_tag="RHV13_000886"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HDV1240441.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQISLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            21129..22460
                     /gene="argA"
                     /locus_tag="RHV13_000887"
     CDS             21129..22460
                     /gene="argA"
                     /locus_tag="RHV13_000887"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HDV1240442.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHQHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(22522..24348)
                     /gene="recD"
                     /locus_tag="RHV13_000888"
     CDS             complement(22522..24348)
                     /gene="recD"
                     /locus_tag="RHV13_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HDV1240443.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGEEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYVDERILSAAIATRTERRSGLAALFSSRG"
     gene            complement(24348..27890)
                     /gene="recB"
                     /locus_tag="RHV13_000889"
     CDS             complement(24348..27890)
                     /gene="recB"
                     /locus_tag="RHV13_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HDV1240444.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLCDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWHDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDVLTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYERHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(27883..30771)
                     /gene="ptrA"
                     /locus_tag="RHV13_000890"
     CDS             complement(27883..30771)
                     /gene="ptrA"
                     /locus_tag="RHV13_000890"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HDV1240445.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISSKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPEEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(30946..34314)
                     /gene="recC"
                     /locus_tag="RHV13_000891"
     CDS             complement(30946..34314)
                     /gene="recC"
                     /locus_tag="RHV13_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HDV1240446.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(34327..34650)
                     /locus_tag="RHV13_000892"
     CDS             complement(34327..34650)
                     /locus_tag="RHV13_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDV1240447.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(34635..35042)
                     /locus_tag="RHV13_000893"
     CDS             complement(34635..35042)
                     /locus_tag="RHV13_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HDV1240448.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIGFSPRGWSDFCPLKEGALCQLP"
     gene            complement(35039..35602)
                     /locus_tag="RHV13_000894"
     CDS             complement(35039..35602)
                     /locus_tag="RHV13_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HDV1240449.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVNASYSVTGFNL"
     gene            complement(35593..36063)
                     /locus_tag="RHV13_000895"
     CDS             complement(35593..36063)
                     /locus_tag="RHV13_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HDV1240450.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(36248..37042)
                     /gene="thyA"
                     /locus_tag="RHV13_000896"
     CDS             complement(36248..37042)
                     /gene="thyA"
                     /locus_tag="RHV13_000896"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDV1240451.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(37049..37924)
                     /gene="lgt"
                     /locus_tag="RHV13_000897"
     CDS             complement(37049..37924)
                     /gene="lgt"
                     /locus_tag="RHV13_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDV1240452.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(38075..40321)
                     /gene="ptsP"
                     /locus_tag="RHV13_000898"
     CDS             complement(38075..40321)
                     /gene="ptsP"
                     /locus_tag="RHV13_000898"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDV1240453.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYADAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(40334..40864)
                     /gene="rppH"
                     /locus_tag="RHV13_000899"
     CDS             complement(40334..40864)
                     /gene="rppH"
                     /locus_tag="RHV13_000899"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HDV1240454.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
BASE COUNT         9892 a        11174 c        10202 g         9811 t
ORIGIN      
        1 taaaagtatt gattttttca gttcaaccta catatattgc gcgccccgga agaagtcaga
       61 tgtcgtttaa tgggaaaata ttgcccttaa attctctttt acttttgatt tacagagtaa
      121 agcgttggga taatctatct tccaagtaga ttattgtatt tgggatcaag atcactgata
      181 gatacataac ttgtgtgtat ctttccgccc tcaaattatt acggcggtaa atgattaagc
      241 catcgccgat agacagattt catttttacg gtcaggcacc ttcccgggct gaactggcta
      301 aaagctgaat tatttgcatt cctccaggag aaatagatgg aaacgactca aaccagcacg
      361 attgcgtcga aagactctcg tagtgcctgg cgcaagacag acaccatgtg gatgctgggc
      421 ctttacggca cggcaatcgg cgcgggcgtg ctgttcctgc caatcaacgc cggtgttggc
      481 ggtatgatcc cgctgatcat catggctatc cttgcgttcc cgatgacgtt ttttgctcac
      541 cgcggcctga ctcgcttcgt actgtctggt aaaaacccgg gcgaagacat caccgaggtt
      601 gtagaagaac actttggtat tggcgcaggt aaactgatta ccctgctcta cttcttcgct
      661 atctacccga tcctgctggt ttatagcgtg gcaatcacca atacagttga aagcttcatg
      721 tctcaccagc tgggtatgac gccaccgccg cgtgcgattc tgtcgctgat cctgatcgtg
      781 ggtatgatga ccatcgttcg cttcggtgag cagatgatcg ttaaagcgat gagtattctg
      841 gtattcccgt ttgttggcgt actgatgctg ctggctctgt acctgatccc gcagtggaac
      901 ggcgcagcac tggaaacgct gtctctggac actgcatctg caaccggaaa cggtctgtgg
      961 atgaccctgt ggctggcaat tccggtaatg gtgttctcgt tcaaccactc tccgatcatc
     1021 tcttctttcg ccgttgcgaa gcgtgaagag tacggcgata tggcagaaca gaaatgctcg
     1081 aagatcctgg cattcgcaca catcatgatg gtgctgaccg taatgttctt cgtcttcagc
     1141 tgcgtactga gcctgactcc ggcagacctg gctgcggcta aagagcagaa catctcgatt
     1201 ctgtcttacc tggctaacca ctttaacgca ccggttatcg cgtggatggc tccgattatc
     1261 gcgattatcg ctatcaccaa atccttcctc ggccactacc tgggcgcacg tgaaggcttc
     1321 aacggtatgg tgattaaatc tctgcgtggt aaaggtaagt ctattgaaat caacaagctg
     1381 aaccgtatca ctgcgctgtt catgctggta acgacctgga ttgttgccac cctgaacccg
     1441 agcatcctgg gtatgattga aaccctgggc ggcccaatca tcgcgatgat cctgttcctg
     1501 atgccgatgt acgcaattca gaaagtaccg gcaatgcgta agtacagcgg tcacatcagc
     1561 aacgtattcg ttgtcgtgat gggtctgatt gcaatctccg caatcttcta ctctctgttc
     1621 agctaagtcc tttcgcgccg ctttcgggcg gcgcttcctc cgttttaacg cgatgtattt
     1681 cctatgatta gcgtattcga tattttcaaa atcggcattg gcccttccag ttctcatacc
     1741 gttggaccaa tgaaagcggg taaacaattt accgacgatc tgattgcccg taacctgctt
     1801 aaagacgtga cccgcgtggt ggttgacgtg tacggctcgc tctctctgac tggtaaaggc
     1861 caccacactg atatcgccat tattatgggc ctggcgggta acctgccgga taccgtggat
     1921 atcgattcca tccctggttt tattcaggat gtgaatactc atggtcgcct gatgctggca
     1981 aacggtcagc atgaagtgga gttcccagtt gatcagtgca tgaacttcca tgccgacaac
     2041 ctttctctgc atgaaaatgg tatgcgcatt accgcgctgg cgggtgataa agtcgtttac
     2101 agtcagactt actactctat tggcggtggc tttatcgttg atgaagagca ttttggccag
     2161 caggatagcg caccggttga agttccttat ccgtacagtt cagcagccga tctgcaaaaa
     2221 cattgtcagg aaaccgggct gtcactctct ggcctgatga tgaaaaacga gctggcgctg
     2281 cacagcaaag aagagctgga acagcacctg gcgaacgtct gggaagtcat gcgcggcggt
     2341 attgagcgcg gtatatccac cgaaggcgtg ttgcctggca aactgcgcgt accacgccgt
     2401 gctgcggcac tacgccggat gctggtcagc caggataaaa ccaccactga cccgatggcg
     2461 gttgttgact ggatcaacat gtttgcactg gcagtgaacg aagagaacgc tgctggcggt
     2521 cgcgtggtga ctgcgccgac taacggtgcg tgcgggatta tcccggctgt actggcgtac
     2581 tacgataagt ttatccgcga agtgaacgct aactcactgg ctcgttacct gctggtagcc
     2641 agcgccattg gttctcttta taagatgaac gcgtcgattt ctggtgccga agtgggttgc
     2701 cagggtgaag ttggtgtggc gtgctcaatg gcggcggctg gtctggcaga actgttaggt
     2761 gcaagcccgg cgcaggtgtg catcgcggcg gaaatcgcca tggagcacaa cctcggtctg
     2821 acgtgtgatc cggtcgccgg acaggtacag gtgccatgca tcgagcgtaa cgccattgcg
     2881 gcagtaaaag cggtgaacgc cgcacgtatg gcgctgcgcc gtaccagcga gccgcgcgtc
     2941 tgcctcgata aagttatcga aaccatgtac gaaacaggta aagatatgaa cgccaagtac
     3001 cgcgaaacct ctcgcggcgg cctggcaatg aagatcgttg cctgcgatta atcgctctcc
     3061 agaggcctcg ttttgcgagg cctcttcccg atttctcatc cagccatagc ctgttccagt
     3121 atcgaatgtt acccttatcg cctgatcttt aagggggtta tcgtggctgt tcatttgctt
     3181 attgtcgatg cactgaatct tattcgtcgc attcatgccg ttcagggatc gccctgtgtc
     3241 gaaacctgcc agcatgcgct cgatcagctc attatgcaca gccagccaac ccacgcggtc
     3301 gccgtttttg atgatgaaaa ccgcagtagc ggctggcgtc atcagcgttt accagattac
     3361 aaagcgggtc gaccgccaat gccggaagag ttgcacgacg agatgcctgc attacgcgcc
     3421 gcctttgagc aacgcggcgt cccgtgctgg tcaaccagcg gcaacgaagc cgatgactta
     3481 gccgccacgc tggcggtcaa agtgacacag gccgggcatc aggcaacgat tgtttcgaca
     3541 gataaaggct actgtcagtt actttcaccg acattacgta ttcgtgatta cttccagaaa
     3601 cgttggctgg atgcgccatt tatcgataaa gaatttggcg ttcaaccgca gcagttgccc
     3661 gattactggg gacttgcggg gatcagcagt tcaaaggtac cgggtgttgc gggaatcgga
     3721 ccaaaaagcg ccacgcagct gctggtcgag tttcagagtc tggaagggat atatgagaat
     3781 ctggatgcgg ttgccgaaaa gtggcgcaaa aaattagaaa cccataaaga gatggcgttt
     3841 ctgtgccgcg atattgcccg cttacaaacc gatttgcata tcgatggcaa tttacagcaa
     3901 ttgcgattgg tacggtaacg gcgagccgga tacgccgcaa acgtcgtatc cggcattatc
     3961 acatcagcgc atttaccagg cggtatggta aagctctaca atatcctcaa gcgttgcttc
     4021 acgcgggttg ccaccggtac aaacatcatc cagtgccgcc tgcgccagtg ccggaatgtc
     4081 ttccttgcgt accccaacat cacgcaaatg tggcggaata ccgacatcac ggttgagagc
     4141 aaacaccgct tcaacagcgg cattacgcgc ctcttccagg ctcatacctt ccactttcac
     4201 gcccataacg cgcgcgatat cgcggtactt ctcaccggta aagtcagcgt tatagcgcat
     4261 gacatgcggt aacaggatgg cgttcgcaac accgtgtgga gtgttgtaaa acgcgcccag
     4321 tggatgcgcc ataccatgca ccaaccctaa cccaacattc gagaagccca tacccgcaac
     4381 atactgcccg agcgccattt cttctccggc atccttttca ccagcaaccg atcctcgcag
     4441 cgccccagca atgatttcaa tcgctttaat gtgcagcgca tcagttagcg cccacgcgcc
     4501 acgggtaata tacccctcaa tcgcatgagt gagcgcatcg acacccgtcg cagctttcag
     4561 cgctggaggc ataccatcca tcatatcagc gtcaataaac gccacctgcg ggatatcatg
     4621 cggatcaacg caaacaaact tgcgccgttt ttcttcgtca gtgatcacgt agttaatggt
     4681 cacttctgcc gcagtgcctg ctgtggtggg gattgccaga atcggtacac tgggtttatt
     4741 ggtcggggaa agtccttcca ggctacgcac atcggcaaac tctgggttgt tgctgataat
     4801 gccaatcgct ttacaagtat cctgtggaga accaccacca atagcgatca ggtaatccgc
     4861 gccgctattc tggaatacac cgagcccttc tttgacgaca gtaattgttg ggttgggcac
     4921 tacgccgtcg taaatcgccc atgccagccc tgcagcatcc atcttatcga tcactttcgc
     4981 caccacgccg cattgcacca gcgttttatc ggtgacgatc agcgccttct gataaccacg
     5041 gcgtttcacc tcatcggtta aagccccaac agcaccccga ccaaaccatg ccgtttcgtt
     5101 cagaatcatt ctgttagcca tcatccttct ccttgttgct ttacgaaatt actcttcaat
     5161 ccgtaaccca taggttttga atttctccag cactacggca atctcttcat cgctcagtac
     5221 tggcaccggg tccgtaatcg ccagggtcgt caggtaaagt tgcgccagca cttcaacttc
     5281 atgcgccagc cataacgctt tttccagatt cacctcacaa gcgataagcc catgatgttg
     5341 taacaaagtt gccttacgat ttttgagagc cagcgcaaca tgttcagaaa gttcgcgtgt
     5401 tccaaaggtc gcataaggcg cgcaaggaat agaattaccg ccagccgccg caatcatgta
     5461 gtgaatagcg gggatcggtc ggttaagaat ggaaactgct gtacaatgaa cggcatgatt
     5521 gtgaacaacc gcgttggcat ccggtctgct ttgataggcc gccatatgga aacgccactc
     5581 gcttgagggg agctttcctt cctcatgttt accgttgcca tcaataaaga caatatgcga
     5641 ctccgtcagt ttttcatatg gaatgcctgt aggcgtaatc agcatcccat cctgataacg
     5701 tacactgacg ttccccgctg tcccctggtt cagtcccagg cgggtcattt ccaggcaagt
     5761 gtcaataatc tgacgagcaa gtttatttcg ttccattagc tacctctctc tgattcaaaa
     5821 cagggcaata atgttgttcc tttcacacta ttgaattagc cgtttaatta cccaccatct
     5881 tcttcctgat taaccagaaa gaaattcaca agcttatatt ttgtgacctg gttcaactaa
     5941 tcacagtaaa taactgcaag ttctcttttt ataaccccat taaaaatgac cgctcttaaa
     6001 aatatttatc aaaacggtca ttttctattc ctccaagccc ggaatgaccg ttttcggcac
     6061 aaacaattaa tacggtcatc tgatttgtgt tttttatgat ttattttctg aaacgggcat
     6121 gaaatttcga ttattaaagt gatggtagtc acataaagtc accttccagc taataagtgt
     6181 gaccgccgtc atattacaga gcgtttttta tttgaaaatg aatccatgag ttcatttcag
     6241 acaggcaaat attcactgat atgaagcccg aactcgctgg ttttgcactt ttgaaaacat
     6301 aaccgattac gtgcttaagc ttctgaacct aagaggatgc tatgggaaac acatcaatac
     6361 aaacgcagag ttaccgtgcg gtagataaag atgcagggaa aagcagaagt tacattattc
     6421 cattcgcgct gctgtgctca ctgttttttc tttgggcggt agccaataac cttaacgaca
     6481 ttttattacc tcaattccag caggctttta cgctgacaaa tttccaggct ggcctgatcc
     6541 aatcggcctt ttactttggt tatttcatta tcccaattcc tgctgggata ttgatgaaaa
     6601 aactcagtta taaagcagga attattaccg ggttattttt atatgccttg ggtgctgcat
     6661 tattctggcc cgccgcagaa ataatgaact acaccttatt tttagttggc ctatttatta
     6721 ttgcagccgg attaggttgt ctggaaactg ccgcaaaccc ttttgttacg gtattagggc
     6781 cggaaagcag tggtcacttc cgcttaaatc ttgcgcaaac atttaactca tttggcgcaa
     6841 ttatcgcggt tgtctttggg caaagtctta ttttgtctaa cgtgccacat caatcgcaag
     6901 acgttctcga taaaatgtct ccagagcaat tgagtgcgta taaacacagc ctggtattat
     6961 cggtacagac accttatatg atcatcgtgg ctatcgtgtt actggtcgcc ctgctgatca
     7021 tgctgacgaa attcccggca ttgcagagtg ataatcacag tgacgccaaa caaggatcgt
     7081 tctccgcatc gctttctcgc ctggcgcgta ttcgccactg gcgctgggca gtattagcgc
     7141 aattctgcta cgttggcgca caaacggcct gctggagcta tttgattcgc tacgctgtag
     7201 aagaaattcc aggtatgact gcaggctttg ccgctaacta tttaaccgga accatggtgt
     7261 gcttctttat tggtcgtttc accggtacct ggctcatcag tcgcttcgca ccacacaaag
     7321 tcctggccgc ctacgcatta atcgctatgg cactgtgcct gatctcagcc ttcgctggcg
     7381 gtcatgtggg cttaatagcc ctgactttat gcagcgcctt tatgtcgatt cagtacccaa
     7441 caatcttctc gctgggcatt aagaatctcg gccaggacac caaatacggt tcgtccttca
     7501 tcgttatgac catcattggc ggcggtattg tcactccggt catgggtttt gtcagtgacg
     7561 cggcgggcaa catccccact gctgaactga tccccgcact ctgcttcgcg gtcatcttta
     7621 tctttgcccg tttccgttct caaacggcaa ctaactgaac atattttccg aataaagtga
     7681 ggaatctgta atgaaaaaaa tcagcttacc gaaaattggt atccgcccgg ttattgacgg
     7741 tcgtcgcatg ggtgttcgtg agtcgcttga agaacaaaca atgaatatgg cgaaagctac
     7801 ggccgcactg ctgaccgaga aactgcgcca tgcctgcgga gctgccgtcg agtgtgtcat
     7861 ttccgatacc tgtatcgcgg gtatggctga agccgctgct tgcgaagaaa aattcagcag
     7921 ccagaatgta ggcctcacca ttacggtaac gccttgctgg tgctatggca gtgaaaccat
     7981 cgacatggat ccaacccgcc cgaaagccat ttggggcttt aacggcactg aacgtcccgg
     8041 cgctgtttac ctggcagcgg ctctggcagc tcacagccag aaaggcatcc cggcattctc
     8101 catttacggt catgacgttc aggatgccga tgacacatcg attcctgccg atgttgaaga
     8161 aaaactgctg cgctttgccc gcgccggttt ggccgtcgcc agcatgaaag gtaaaagcta
     8221 tctgtcgctg ggcggcgttt cgatgggtat cgccggttct attgttgatc acaacttctt
     8281 tgaatcctgg ctgggaatga aagtccaggc ggtggatatg accgaactgc gtcgccgtat
     8341 tgatcagaag atttacgacg aagccgaact ggaaatggca ctggcctggg ctgataaaaa
     8401 cttccgctat ggcgaagatg aaaataacaa acagtatcaa cgcaatgccg agcaaagccg
     8461 cgcagttctg cgcgaaagtt tactgatggc gatgtgtatc cgcgacatga tgcaaggcaa
     8521 cagcaaactg gccgatattg gtcgcgtgga agaatcactt ggctacaacg ccatcgctgc
     8581 gggcttccag gggcaacgtc actggaccga tcaatatccc aatggtgaca ccgccgaagc
     8641 gatcctcaac agttcatttg actggaatgg cgtgcgcgaa ccctttgtcg tggcgaccga
     8701 aaacgacagt cttaacggcg tggcaatgct aatgggtcac cagctcaccg gcaccgctca
     8761 ggtatttgcc gatgtgcgta cctactggtc accagaagca attgagcgtg taacggggca
     8821 taaactcgat ggactggcag aacacggcat catccatttg atcaactcag gttctgctgc
     8881 gctggacggt tcctgtaaac aacgcgacag cgaaggtaac ccgacgatga agccacactg
     8941 ggaaatctct cagcaagagg ctgacgcttg cctcgccgct accgaatggt gcccggcgat
     9001 ccacgaatac ttccgtggcg gcggttactc ttcccgcttc cttaccgaag gcggcgtccc
     9061 gttcaccatg actcgtgtca acatcatcaa aggcctggga ccggtactgc aaatcgcgga
     9121 aggctggagc gtggaattgc cgaaggatgt gcatgacatc ctcaacaaac gcaccaactc
     9181 aacctggcca accacctggt ttgcaccgcg cctcaccggt aaagggccgt ttacggatgt
     9241 gtactcggta atggcgaact ggggcgctaa ccatggggtt ctgaccatcg gccacgttgg
     9301 cgcagacttt atcactctcg cctccatgct gcgtatcccg gtatgtatgc acaacgttga
     9361 agagaccaaa gtgtatcgtc cttctgcctg ggccgcgcac ggcatggata ttgaaggcca
     9421 ggattaccgc gcttgccaga actacggtcc gttgtacaag cgttaataca ttactccctg
     9481 atgtgatgcc cggtcgctcc ggctaccggg cctgaacaag caagagtggt tagccggata
     9541 agcaatgtta tccggctata ttgcaggagc gattatgaaa caagaagtta tcctggtact
     9601 cgactgtggc gcgaccaatg tcagggccat cgcggttaat cggcagggca aaattgttgc
     9661 ccgcgcctca acgcctaatg ccagcgatat cgcgatggaa aacaacacct ggcaccagtg
     9721 gtctttagac gccattttgc aacgctttgc tgattgctgt cggcaaatca atagtgaact
     9781 gactgaatgc cacatccgcg gtatcgccgt caccaccttt ggtgtggatg gcgctctggt
     9841 agataagcaa ggtaatctgc tctatccgat cattagctgg aaatgtccgc gaacagcagc
     9901 ggttatggac aatattgaac ggttaatctc cgcacagcgg ttgcaggcta tttctggcgt
     9961 cggagccttt agtttcaata cgttatataa gttggtgtgg ttgaaagaaa atcatccaca
    10021 actgctggaa cgcgcgcacg cctggctctt tatttcgtcg ctgattaacc accgtttaac
    10081 cggcgaattc actactgata tcacgatggc cggaaccagc cagatgctgg atatccagca
    10141 acgcgatttc agtccgcaaa ttttacaagc caccggtatt ccacgccgac tcttccctcg
    10201 tctggtggaa gcgggtgaac agattggtac gctacagaac agcgccgcag caatgctcgg
    10261 cttacccgtt ggcataccgg tgatttccgc aggtcacgat acccagttcg ccctttttgg
    10321 cgctggtgcc gaccaaaatg aacccgtgct ctcttccggt acatgggaaa ttttaatggt
    10381 ccgtagcgcc caggttgata cttcgctgtt aagtcagtac gccggttcca cctgcgaact
    10441 ggatagccag gcagggttgt ataacccagg tatgcaatgg ctggcatccg gcgtgctgga
    10501 atgggtgaga aaactgttct ggacggctga aacaccctgg caaatgttga ttgaagaagc
    10561 tcgtctgatc gcgcctggcg cggatggcgt aaaaatgcag tgtgatttat tgtcgtgtca
    10621 gaacgctggc tggcaaggag tgacgcttaa taccacgcgg gggcatttct atcgcgcggc
    10681 gctggaaggg ttaactgcgc aattacagcg caatctacag atgctggaaa aaatcgggca
    10741 ctttaaggcc tctgaattat tgttagtcgg tggaggaagt cgcaacacat tgtggaatca
    10801 gattaaagcc aatatgcttg atattccggt aaaagttctc gacgacgccg aaacgaccgt
    10861 cgcaggagct gcgctgttcg gttggtatgg cgtaggggaa tttaacagcc cggaagaagc
    10921 ccgcgcacag attcattatc agtaccgtta tttctacccg caaactgaac ctgaatttat
    10981 agaggaagtg tgaaatgctg aaaacaattt cgccgttaat ttctcccgaa ctattgaaag
    11041 tgctggcaga gatgggacat ggagatgaaa ttattttttc cgatgctcac tttcccgccc
    11101 attcgatggg accgcaggtg atccgcgctg atggcctgtt ggtgagcgac ttgctccagg
    11161 cgattatccc gttatttgaa ctggacagtt atgcaccgcc gctggtgatg atggcggcgg
    11221 tagaaggtga cactctcgat cctgaagtag aacaacgtta ccgtaatgcg ctttcactac
    11281 aagccccgtg tcctgacatc atccgcatca atcgttttgc gttttatgaa cgggcgcaaa
    11341 aagcctttgc gatcgttatc acaggcgaac gagcgaagta cgggaatatt cttttaaaaa
    11401 aaggggtaac accgtaatct cataccggta cgcccgcatg acgcgggcgg ttatcgaatg
    11461 atggggtgaa aaatatgaaa gcggcacgcc agcaagcgat agtcgacctg ctgctgaacc
    11521 ataccagcct gaccacggaa gctctctctg aacagctaaa ggtcagtaaa gaaaccattc
    11581 gtcgcgatct caatgaatta cagacgcagg gtaaaattct gcgcaatcat ggacgcgcta
    11641 aatatatcca ccgtcaaaat caagacagtg gcgatccctt tcacatcagg ctgaaaagcc
    11701 attatgcgca taaagcagat atcgcgcgcg aggcgctcgc gtggattgaa gaagggatgg
    11761 tgatagcctt agacgccagt tcaacttgct ggtatctggc acgccagttg cctgacatca
    11821 acattcaggt cttcaccaat agccatccga tttgccatga actcggtaaa cgcgaacgca
    11881 ttcaactgat cagttccggc ggcacacttg agcgcaaata tggctgttac gtcaatccct
    11941 cgctgatttc ccaacttaaa tcgctggaaa tcgatctgtt tattttttct tgtgaaggga
    12001 tcgatagcag cggcgcactg tgggactcca atgccatcaa cgctgattac aaatcgatgc
    12061 tattaaaacg tgccgcgcaa tcgttgttat tgattgataa aagtaaattt aatcgttcag
    12121 gggaagcccg catcgggcat ctggatgagg taacgcacat tatttctgat gagcgccagg
    12181 ttgcaacttc tttggtaaca gcctgacgga aagggtagca ggccggagac gacgccccgg
    12241 ccttgcctgt tatcgctcgt cgcgacgacc acccaccgca gcccagatgc ggcggacgtg
    12301 caccgtcact tcttcgcgat cgtgatacaa ctgccgcgcc tgaatctgag catttatgcc
    12361 atgttcatca agctgtgcct gaatatacgc cagattgtgt gacacttctt cgtagcgttt
    12421 tttcatcggc agtttgaggt tgaaaatggt ttcacgacac cagccattaa ccagccactg
    12481 cgccatcaat gccgcaactt tcgccggttt ttcaaccata tcgcatacca tccaggagat
    12541 attgctgcgc gtcggacgga atttgaaacc gtcttcccgc agccacgtca cctgtccggt
    12601 atccatcaga ctttgcgcca tcgggccgtt gtcgacggaa taaacccaca tgttgcgctt
    12661 caccagttgg taggtccagc cgccagggca agcgcctaaa tccaccgccc acatcccgtt
    12721 cgccaggcgt tcatcccact catccgcagg aataaacaca tgaaatgcct cttccagttt
    12781 gagcgtggaa cgactcggcg catctgccgg aaatttcagg cgcggaatgc ccatatagaa
    12841 cggcgaatta ttgttgctgt atgagtaacc ggtatagcag cagcctggtg caataaagaa
    12901 tacatgcaca accggacgct tcggcgtttc atagttcgcc agcaccccag catcgcgcaa
    12961 ggcagcgcgt agcggcacgg tgaatttacg gcagaatttc agcaactctt tgctttcgtt
    13021 ggtatcggca acttcaacac gcagttcacc gcccttctct actacgccct gtaacatgcc
    13081 gacaatgggg gtaatacgat cttctggcgg caaatgctgc aggagttccc ccaccacaaa
    13141 ccactggcgg gcaaaaatta atgaactgaa cggcagctca cggattaact tatcgccatc
    13201 atcaggttga taacattcat aaatgacata acccgcattc tctttcacgc gggcaaaacc
    13261 gaaaatttcc cgctggccgg ctttatcggt aatttctgcg gcgcactctt tttcaaagcc
    13321 cggacggcac agcaatacaa ccttattcat gacttacgcc cttgcgcttt aaacggatag
    13381 caccaactaa cattaacgcc cagcccgcaa ggaagctcac gccgccaacc ggagtgacaa
    13441 acgcccacaa acgcagatgg gacagtgcca ggcaataaag gctgccgctg aacagcaccg
    13501 tgcctaacgc gaggaaaacg ctactccagt aaaaccagat actgatgcga cgctgcattg
    13561 ccaccgccag acctaagatc gccagcgtat gaaacgcctg gtattcgagg ccggtctgga
    13621 tccagcccat ctcaacggcc cccatggttt tacttaacac atgcgcgcca aaagcgccca
    13681 gtgccacaaa aatgaagccg ctaatggcgg cgaaaatcag cataaaacgg ctggtcatgg
    13741 tcgtacccta cgtaaattat tgttcataac gaaagcggaa tttttcttgt tcagcagcgg
    13801 ctttcgccag gatccattgg cgaaaggcgg ctattttacc cagttctgcc tggctgtcat
    13861 gacaaaccag ataaaaagcg tttttactga ccagaacatc attaaacggg caaacaagac
    13921 gtccggcttc aatttcagat tgcgccatca cgttatttgc cagcgccact ccctgcccgt
    13981 ggatagccgc ttgcagcacc atggcgctat ggctaaaaat tggcccttgc tgaacgttga
    14041 tatgatttaa ccccaattga cgagtatatg tctgccagtc acggcgtgac gcatcatgta
    14101 atagcgtatg tttagccaga tcttccgggg tcttcaaggg tttttcgcca gtcagcagta
    14161 gcggcgaaca caccggcaat aaatattcgg cgtacagttt ttccacccgt agccccggcc
    14221 agttgccccg accataaaat atcgccacat caacatcatc cgccagctta tcttcctgac
    14281 gatcaaccgc ctggattcga acgtcaattc ccggataagc tgaattaaag ctggaaagtc
    14341 gcggaaccaa ccaatgaatg gcgaaactgg ggagtaaact gaccgtcaac gcccccttgg
    14401 cgctacgggc ctggagttta cgcgtcgctt cggttaattg cgaaaatatc tctttgatat
    14461 cgaggaaata gctttgacct tcctcggtca gcaggagtga acgattacgg cggcggaaca
    14521 gttttagccc cagaaaatcc tcaagagact tgatttgatg acttactgcg gcctgggtca
    14581 caaaaagctc ttctgctgcg cgagtgaaac ttaaatggcg tgctgcggca tcaaaaactc
    14641 gtaaggcatt tagcggtggt aatcgtttag acatggcttt taaactttga tgttaaatga
    14701 atttaacaat tagatcacac tgtgtaacct attagttttt ttaatctgag ccattataaa
    14761 ttgtccgttg agcttctacc agcaaatacc tatagtggcg gcacttcctg agccggaacg
    14821 aaaagtttta tcggaatgcg tgttctggtg aacttttggc ttacggttgt gatgttgtgt
    14881 tgttgtgttt gcaattggtc tgcgattcag accatggtag caaagctacc ttttttcact
    14941 tcctgtacat ttaccctgtc tgtccatagt gattaatgta gcaccgccta attgcggtgc
    15001 ttttttttac cttgcgatcg cgaattactg atccagttcg accatctctt tcacgtcggt
    15061 acggttgatc tgctgtttgt tgccattagc gtctttatac gaaatcatac cggtatcgtt
    15121 atcagtctgt ggtttgccgt cagagacgat ggtacgtccg tcattggtgt gcatcacata
    15181 gttcgaaccg gaacaggcgc tcagggcaaa agtcagcata caggcagaaa taattgcggc
    15241 agtctttttc atcatcttct ccttaaagcg attaataaat cttaatagta ttgattaaca
    15301 ggctaaaatt aacgcctaac actattcagc atatgttact gacgcggctt cgccaggata
    15361 tccagataat tctgatggtt agcactctcc ttgtatcaaa gtgaattttg cgtcacgatc
    15421 ggtgcatcaa gccgaggagt accatgaacg tttttaatcc cgcgcagttt cgcgcccagt
    15481 ttcccgcact acaggatgcg ggcgtctatc tcgacagcgc cgcgaccgcg cttaaacctg
    15541 aagccgtggt tgaagccacc cgacagtttt acagcctgag cgccggaaac gtccatcgca
    15601 gccagtttgc cgaagcccaa cgcctgaccg cgcgttatga agctgcacga gagaaagtgg
    15661 cgcaattact gaatgcaccg gatgataaaa ctatcgtctg gacgcgcggc accactgaat
    15721 ccatcaacat ggtggcacaa tgctatgcgc gtccgcgtct gcaaccgggc gatgagatta
    15781 ttgtcagcgt ggcagaacac cacgctaacc tcgtcccctg gctgatggtc gcccaacaaa
    15841 ctggagccaa agtggtgaaa ttgccgctta atgcgcagcg actgccggat gtcgatttgt
    15901 tgccagaact gattactccc cgtagtcgga ttctggcgtt gggtcagatg tcgaacgtta
    15961 ctggcggttg cccggatctg gcgcgagcga ttacctttgc tcattcagcc gggatggtgg
    16021 tgatggttga tggtgctcag ggggcggtgc atttccccgc ggatgttcag caactggata
    16081 tcgatttcta tgctttctct ggtcacaaac tgtatggccc gacgggtatc ggcgtgctgt
    16141 atggcaaatc agaactgctg gaagcgatgt cgccctggct gggtggcggc aaaatgattc
    16201 acgaagtgag ttttgacggc ttcacgactc aatctgcgcc gtggaaactg gaagcaggaa
    16261 cgccaaatgt ggctggtgtc ataggattaa gcgcggcgct ggaatggctg gcagattacg
    16321 atatcaacca ggccgaaagc tggagccgta gtttagcaac actggcggaa gatgcgctgg
    16381 cgaaacgtcc aggcttccgt tcattccgct gtcaggattc cagcctgctg gcctttgatt
    16441 ttgctggcgt tcatcacagc gatatggtga cgctgctggc ggagtacggt attgccctgc
    16501 gcgccgggca acattgcgct cagccgctac tggcagaatt aggcgtaacc ggcacactgc
    16561 gcgcctcttt tgcgccatat aatacaaaga gtgatgtgga tgcgctggtg aatgccgttg
    16621 accgcgcgct ggaattattg gtggattaat gacgaacccg caattcgccg gacatccgtt
    16681 cggcacaacc gtaaccgcag aaacgttacg caataccttc gcaccgttga cgcaatggga
    16741 agataaatat cgccagttga tcatgctggg gaaacagctt ccggctttgc cagacgagtt
    16801 aaaagcgcag gctaaagaga ttgccggatg cgaaaaccgc gtctggctgg gatataccgt
    16861 ggctgaaaac ggtaaaatgc acttctttgg cgacagcgaa ggacgtatag tgcgcggcct
    16921 gctggcggtg ctgttgactg ccgttgaggg gaaaaccgcc gccgaattgc aggcacagtc
    16981 gccgctggca ttgtttgatg agctgggatt gcgtgcgcaa cttagcgcct cacgcagcca
    17041 ggggttaaat gcgttaagcg aggcgattat cgctgcgacg aagcaggttt aagcacagaa
    17101 ccgggtcgga taagacgttc gcgtctcatc cgacctgatt gtttaaccct gacgcgccgc
    17161 tttcgccatc atcttcttca gcgcatgaga aactgcaaca aacccaaagg tggcggtcac
    17221 catcgttgcc gcgccaaatc cagatgcaca atccatccgc tttggccctt ccgccgtggc
    17281 tttcatagca cacaccgtac cgtctgactg cggatacact agcgcttcag tagagaacac
    17341 gcaatcgacg ccgagcttac ctttactgtt tttcactacg ccaaaatcgc ttttcaggcg
    17401 ctcgcgcaac ttcgccgcca gcgggtcctg aatcgttttc gccagatcgg taacctgaat
    17461 ctgcgtcggg tcaatctgcc cacccgcgcc accggtcgtg accagcggga ttttattgcg
    17521 ccgacaataa gcaatcagcg ccgctttgga ccgtacacta tcaatggcat caatcacgta
    17581 cgaataaccg gcactcatat attgcgctac gttatccggc gtcacgaaat catccaccac
    17641 cgttacacgg cactccgggt taatctggcg aatacgttcc gccatgactt ccgcttttgc
    17701 cagcccgacg ttatcgcgca gagcgtgaat ttgccgattg gtattggtga cgcacacatc
    17761 atccatatcg ataagcgtga ttgcaccaat ccccgtgcgc gccagcgctt ccgccgccca
    17821 ggaaccgaca ccgccaatac cgaccacaca aatatgcgcg tcagcaaaca gttgcaacgc
    17881 tttttcacca tacagacgcg ctgtgccacc aaaacgctga cgccatgcat cactaattac
    17941 cacagacata gcacctcaga attaaaaagg gcgaggttac ccctcaccct aaacgcataa
    18001 atgcctgatg cgctacgctt atcaggccta cataactcct gcaatatttt ggatttgcat
    18061 gatttgtagg ccggataagg cgttcacgcc gcatccggca tgaacaacgc gcacttagtc
    18121 aataatcagg atgtgaggtt acccctcacc ctgtcatatc cgtaaaaacg gcatacagaa
    18181 tatcacatca gccgctaaag acgttacctg cgcccggggc ggttttcagc acccagacac
    18241 gtccatagtg gttgtaccaa cctgcgcggt gtccggcttc cggcccgatc ccttgataga
    18301 tatcgaagtg ttggcctttg attgcgccac cgacatccag cgccaccatc agacgcaatt
    18361 cgtactgacc gttaaatttg ccgttattat ccagcagcgg cacttctgcc agcaaggttg
    18421 tacctggcgg aataatggaa cgatcagagg caacagaagc acggccaacc agcggcaccg
    18481 cacttgcccc tttcaccggt gcaaaagatt gcggtttaaa gaaaacgaaa gacgggttct
    18541 gttccagcag ctcgcgaacc tcggcttcac tgtgtgtttc gccccagtga cgaatcgcct
    18601 gcatcgacat atcttctttt ttcacttcgc cacggtcaat cagcacctta ccaatgctgc
    18661 gataggcatg accgtttttc cctgcatagc tgaaaaagtt aagcggactg ccatcaccaa
    18721 agtcgatata cccactaccc tgcacatcca taatgaagtt atccatcagg gagttactgt
    18781 aagctagaat atatttatca ctcaatgccc ccgcgtagat ctccgcacga gacggcagac
    18841 gaccacgttt tggcggcata cggtaaatag gatactggaa ctcgccctgg cgggtatggc
    18901 gcgcctgaat taccggcgtg taatagcctg taaactgcac gttaccatag ttgtcggcac
    18961 cttccatctg ccaagcatca atgccgaact ggcgcatatt gcgggtatca ccgcctgcgc
    19021 gcagccactc ttgcaccgcg ttataaacat tactctggtt gccatacaga cgcggtgacg
    19081 aattacggat atggttaatt tgctcggcaa aatcaccggc gttaatcggc gcgccaacgg
    19141 catctggctg gttcaccaga gagaaaggct gggtaaattt cccgtcttta tattgctgtc
    19201 cgcgatcggt tggtttggaa gagcaggcgg caagcattgc cacaaccgtg cccataagaa
    19261 ggtactttac ccaacgtcct ttcattgttc tcttcttcag gttaaaaaat aaggcgcaac
    19321 gaagataaca aaccgccgcg tttaatgaaa tgagcagcct ctccctgacg caaattttgc
    19381 acaaaaaata ggctttagtg atttgttttt gttcaaaatc atgccagatc cgtgatcggg
    19441 gtaaaaaaaa ggttgcatga aaacgcgagc ggagtatagt gcgcatccac ggacgcgggg
    19501 tggagcagcc tggtagctcg tcgggctcat aacccgaagg tcgtcggttc aaatccggcc
    19561 cccgcaacca attattgaac accctaacgg gtgttttttt gtttctggtc tcctataaaa
    19621 aagcgccatt cagcgccttt ttatcatccc cttctcgcca gcgtcgcccc atcggcaaaa
    19681 tacgctttaa tccctgcaag aatagactcc gcaacttcct gctggaaggt cgccgttttc
    19741 agtttacgct cttcctcaac gttacttata aacgccgttt cgaccagaat ggagggaata
    19801 tctggtgcct ttagtactgc aaacccggcc tgttcaactt gatttttatg cagcttgttg
    19861 attttaccga gcttattcag caccgcttta ccaaacttaa ggctgtcagc aatggtcagc
    19921 gactgcacca tatcgaacat ggtgtggtcg acatagcggt caccgctttt gctcacgcca
    19981 ccaatcaagt cggaggcgtt ctgggtttgt gccagatatt ttgccgcagt actggttgca
    20041 cctttggttg agagcgcaaa caccgaggaa ccgctcggct ggcgactggt aaaggcgtcg
    20101 gcatggatag agacaaacag gtcagcacgc tgtttctgag cttttgctac gcgcacttgc
    20161 aacggaatga agatgtcttc attgcgcgtc atgtacacct tcatattgcc ctctttctcg
    20221 atcagagagc gcaaacggcg agctatttgc aataccacat ctttttcgcg cgttttgtat
    20281 ttccccaccg caccggagtc ttcgccaccg tggccagggt caagcataat gacaatcgga
    20341 cgatcgcgcc ctgctttacc tggttgtgga ccactttgcg ctggcggcac ctgcttttcg
    20401 aggtcgcctt tgttgtaatc ctccagcagc gccagcagcg ggtcctgcat atcctgtgca
    20461 ttggccggat agaggtccat caccagacgc tctttaaacc cggcgaccgg cgcaagggca
    20521 aacagctgcg gttttacgtt ttgctttaat tcaaaaacca tccgtacggt ttgcgggtca
    20581 aattgcccga cgcgcgccga cttgatgaac gggtcgtctg cacgaatttg tgccgccatc
    20641 cccttgagta ccgagttcag gtttacatct tcgatatcca ccaccacgcg ttcagggtta
    20701 ctcaacgcga actgcttata tttcagctga cgatttgatt ctaccgtcac gcgggtgtag
    20761 ctggacgcag gccagacgcg cactgccacg acctggctga ccgcagccag gctgatctga
    20821 cttacgctca atagccacat ggcacccgcg ccttgcagta aacgacgacg gctgattgca
    20881 gtgttggatc ctgacatgcc tctcccgagc aaaagaaatc taagctgtgt aacaagtaaa
    20941 cgactaattt gaccggaaac tttagcgaaa gacgcataat ctgtcatcta ataaacggta
    21001 aacattcttt ttatattcac ggcattactg ataaaaaagt cgctctcgca taaaatttac
    21061 acttgcaccc tgcgaaaaaa cagaataaaa atacaataat ttcgaataat catgcaaaga
    21121 ggtgtgccgt ggtaaaggaa cgtaaaaccg agctggtcga gggattccgc cattcggttc
    21181 cctatatcaa tacccaccgg ggaaaaacgt ttgtcatcat gctcggcggt gaagccattg
    21241 agcatgagaa tttctccagt atcgttaatg atatcgggtt gttgcacagc ctcggcatcc
    21301 gtctggtggt ggtctatggc gcacgtccgc agatcgacgc aaatctggct gcgcatcaac
    21361 acgaaccgct gtatcacaag aatatacgtg tgaccgacgc caaaacactg gaactggtga
    21421 agcaggctgc gggaacattg caactggata ttactgctcg cctgtcgatg agtctcaata
    21481 acacgccgct gcagggcgcg catatcaacg tcgtcagtgg caattttatt attgcccagc
    21541 cgctgggcgt cgatgacggc gtggattact gccatagcgg gcgtatccgg cggattgatg
    21601 aagacgcgat ccatcgtcaa ctggacagcg gtgcaatagt gctaatgggg ccggtcgctg
    21661 tttcagtcac tggcgagagc tttaacctga cctcggaaga gattgccact caactggcca
    21721 tcaaactgaa agctgaaaag atgattggtt tttgctcttc ccaaggcgtc actaatgacg
    21781 acggtgatat tgtctccgaa cttttcccta acgaagcgca agcgcgggta gaagcccagg
    21841 aagagaaagg cgattacaac tccggtacgg tgcgcttttt gcgtggcgca gtgaaagcct
    21901 gccgcagcgg cgtgcgtcgc tgtcatttaa tcagttatca ggaagatggc gcgctgttgc
    21961 aagagttgtt ctcacgcgac ggtatcggta cgcagattgt gatggaaagc gccgagcaga
    22021 ttcgtcgcgc aacaatcaac gatatcggcg gtattctgga gttgattcgc ccgctggagc
    22081 agcaaggtat tctggtacgc cgttctcgcg agcagctgga gatggaaatc gacaaattca
    22141 ccattattca gcgcgataac acgactattg cctgcgccgc gctctatccg ttcccggaag
    22201 agaagattgg ggaaatggcc tgtgtggcag ttcacccgga ttaccgcagc tcatcacgag
    22261 gcgaggttct gctggaacgc attgccgctc aggcgaagca gagcggctta agcaaattgt
    22321 ttgtgctgac cacgcgcagt attcactggt tccaggaacg tggatttacc ccagtggata
    22381 ttgatttact gcccgagagc aaaaagcagt tgtacaacta ccagcgtaaa tccaaagtgt
    22441 tgatggcgga tttagggtaa gcgacgaaaa tcgccggatg cgacatgcgt aacactcgtg
    22501 cgtcgcatca ggcaattacg tttatccccg tgaactaaac aacgccgcca gaccgctgcg
    22561 ccgctcggtg cgcgtggcaa ttgccgcact taaaatgcgc tcatcgacat acagcgacag
    22621 acggcgacgc gcgcgggtca ccgcagtgta aaccagctct cgtgttacta ctggcgtgcg
    22681 ttggctcggt aaaatcaacg ccgcatggtc gaactccgat ccctgcgatt tatgtaccgt
    22741 catcgcccac gtagtttcgt gctctggcaa gcgactcggt tgcacagact taatattgcc
    22801 gtccggcatc gcaaaccaga cgcgcgtccc ctgcccgcga tccagcgcaa taccgatatc
    22861 gccattaaac aacccaagcg cgctgtcatt acgggcaatc atcaccggtc ggccttcgta
    22921 ccaacgagag tgcggatgac gatgaatttt gcgcttctgt tgcataaact gctcaattcg
    22981 ctcattcagt ccagccacgc caaacggccc ttcccgcagg gcgcacaaaa gctggtactc
    23041 attgaacgcc tgaatgatta aatccggctc ggcacgcgct tgcagcagat ccagataacg
    23101 tccgtaaccc gcaagagctt cctcaagcat cgcaatataa tcttcgccgc tctgtaaaag
    23161 ccgtttttca atatcagtaa aatcctgctg aaaaacggtt ttcactgccg ttttatcacc
    23221 acggttgatc gccgcagcta actgaccaat gccagaatcg ctgccgaaac gatagctttt
    23281 ttgcagcagg cagagactgt cgcgcaaaga tgccgcttct gtgccagttc ctgccggaac
    23341 gtgagttccc gtcaggcggc ttagctgccc ggcacgctcg gcggtaaagc cggcattggc
    23401 ataagcgcag atatcgccca gcacagcccc agcctcaacc gaggccagtt gatcacgatc
    23461 gccgagaaag atcactcgcg catgatcggg caaggcgtcg atcagtctcg acatcatagg
    23521 cagatcgatc attgacgctt catctaccac cagcacatca agatgcagcg ggttaccggc
    23581 atgatgacgt aaacgctggc tacccggctg cgcacccagc aatcggtgca aagtactggc
    23641 atcttccgga atgcgtttct tttgttcatc ggtcagcggt aactgtcgca aagccttgcc
    23701 gagagattcg gttaagcgcg cggcagcttt acccgttggt gcagccagac ggatacggca
    23761 gcgttcgccg tcggccattt gaattaacgc tgccagcaac ttcgccacgg tggtcgtttt
    23821 accggtgcct gggccgccgg aaatcaccga gatccgccgc gtcagcgcca ctgccgctgc
    23881 aactttttgc cagttaattt catcgcttac tggaaaaagt ttgtccaggg tttgcgccag
    23941 tagagcttca tcaacctcga tggcatgatt cacctcattg aagaagcgcg ctaccgtccg
    24001 ctcgttacac cacatgcgat tcaaataaag acgatcgcca cagaggatca tcggcgtggg
    24061 ttcatctccc ctgctgaccg cttgagaagc caacaagcat tcttcccaat tttgtagctc
    24121 accgatttca ctgacacagg tcgccaacag cggatgcgac gcctcgttat tttccagtcg
    24181 tgaaagcggc aaacaaacgt gtccctctcc ggcatcatga cttaacagtg ccgccgcgag
    24241 ggtgacggca ggatgttctt ctcccgccac ggtcagggca aattgcacat ccagcgggcg
    24301 tagctgtttg tgctccacag cttccagtaa ttgcttttgc aatttcatta cgcctcctcc
    24361 agggtcatac cggcaaacat ctcatccatc agggcaatca acccggcgtt gggtcgggtt
    24421 gtgtaaatcc cctgttgcgg atgttcttta tcaacgccac gcaggaacag ataaataacg
    24481 ccgccaaagt ggcgctcata gtcgtaatca gcaatgcgat ggcgcagata acgatgcagc
    24541 gccagggtat aaagctgata ttgcagatca tagcggtgtg cctgcattgc cgctgccata
    24601 gcctgttggg tgtaagccga actgtcttca cccaaccagt tggatttata gtcgagcagg
    24661 taataacgcc cttcgtggcg gaacaccagg tcgataaagc cttttaacat gccgcgtacc
    24721 tgcatgaact ccagcggcgg gcagccagcg gatagcgggt caaactggcg aattagtgca
    24781 tcaagctggc tggcgatgag cggttcgcta atcggcaggt aaaactccat ctccacctgt
    24841 ttatcgcggt cggaaagctg actcaggcta acacccgttt cattgagcgg cgcctggagg
    24901 acagccgtga tccactcggt caacaccggt tcccactgcg attcaaagcc gccgagttcc
    24961 agtttttcct gtacccagtt cgggtcaacc ggctgggtaa aatcgaggtc ttcaaataaa
    25021 ctgtgcaaga atgtccccgg tgacgcaccg cgcgggaact gatgcggtgt taacgtcggt
    25081 tcttcaacga cgctgaccac gcctgcggca tcaacatcca gccgtggcat cagatcctga
    25141 gcgataccgt gaccacgctg ctgcaaaccg gagtagctgg tgacacgcca gttatcgccg
    25201 ggcaatcgtt gtagcgtcct cgcattcagt tctgcagtta aaacatcatt aacctgccag
    25261 ggctggttat caccagtttg tgccgtttgc caggcaatat catcatcgca taacgcttca
    25321 atacaggtgc gaagccctgc cgcatcttgc ggttcccctt tttgcagcaa acgcccgagc
    25381 gcactttggt ggacgtcggt gtcacctttt ttatcgccac gacggcgcac cagcggtgca
    25441 acgccgagac tacaatgcca aaccgaacgc gtcagcgcca cgtaaagcaa acgcagatct
    25501 tccgccagac gttcggcctc cgcgagatcg acactttctg gcgcagcgtt aagatccaga
    25561 acagcctcaa acgagtggcg atcgtgataa aacgcctgat cctggacgcg gaaattggta
    25621 ataaatggca accagaccag cggatattcc agccctttcg atttatggat cgtgacaatc
    25681 tgcaccagat gtttatcact ttcgaggcgc atttgttggc tggaggcatt actgtctggc
    25741 tcgaggatat gttgcgataa ccagcgtacc agggcatgtt cactttccag ctgcgttccg
    25801 gcttcttgta gcagttcgct gatatgcaag atatcggtaa gacgccgctc accgcctgcc
    25861 gttgccagca agttttctgc aatgttacgc gccgacatca gcgcccgcag catcggcata
    25921 acgccacgtt tgcgccaaat ttgccgataa ccatcgaact cttcgactac cgcatcccac
    25981 gcatgttcgt cattgttcag cgtttcaata tccagcgcat tcagccccat cattgacgtt
    26041 gccagcgcac tgcgcagggt gttctcacgt tcgggcgtca tcaccgcctg caacaaccaa
    26101 agcatttcct gcgcttccag agtttcaaaa acactgtcgc ggttcgaaag gtaaacggaa
    26161 gggatttcca gcaacgttaa ggcatcgcgc acctgggcgg cctcctggcg gctgcgcacc
    26221 agcacactga tgtccgaagc acgcaccgga cgcgcgtcgt cgccgttcat cagcaacgct
    26281 tcgccccgct gtccggcttg tagccagtcg cggatttgcg cagcacatac ctgcgccatg
    26341 gtactttgat aatcgccaac gccgcagctt tcgccttcca tcagccacat tttcatcgca
    26401 ggctgcgttt cacctttaaa gacaaaacgt aacgcctgat ttttcccggc tgatttcact
    26461 ggaataaacg gtatttcgcg aaacatgaac gcgtcatcag tctggctgaa aagcttgttc
    26521 acactgttca ccattccagg tgcggagcgc cagttggtgt ctaaagtgta gtgggcgtga
    26581 acttcactac gcgccttcat ataagtgaag atatccgcac cccggaatgc atatatggcc
    26641 tgcttcgggt cgccaatcag caacaatgcg gtttccggct gatggtgcca gatacggcga
    26701 aaaattcggt actgctgggg gtcggtatcc tgaaattcat cgatcattgc caccgggaat
    26761 cgcgtacgga tcgccgctgc caacacctca ccgctttcgc tacgcagcgc ggaatcgagc
    26821 cgacttaaca tgtcatcaaa acccaattcg ccacggcggc gtttttcacg cgctactgtt
    26881 tcgcggatct cagccaatgc gcgggtgatc accagatcgc ggatcgacaa tggttctgca
    26941 agcagttgat cgatcgcctc aaacagtgga tgtcgcgggg tttccccccc ggccttcgtg
    27001 cgatcttcta agaaacgctg ggagaatttt tccagcgact ccggcaactg ataactgttt
    27061 gtctcttctt ctgcccaggc gctgatcttg tcgatccatt tagcctgatt gctacggtta
    27121 aacttgcgtc gatcaatacc agaagattcg atcagcgcat ccagttcacc cactgcgtcg
    27181 tgccactgct gttttaccgt atcaatacgc gccacaattt gcgcgtgacg ggaagccagc
    27241 gtttcatcat cgggcggcgg tgctttgata accggcgctt cgccttgcag ataacgatta
    27301 atatcgcaca gcaacgcctg cggccctttc caggtttcaa agacgacctg ggctatttca
    27361 cgcggcagcg ggtagcagtg gcgacgccag aaatcggcgc aggcctggta gcgtagcaga
    27421 gactcatctt caatcagctg ctgctcaaac agcatgccgg attcaaaggc attcaggttg
    27481 agcatgcgct ggcaaaagcc gtgaatagta aagactgccg cttcatccat ctgccgttcg
    27541 gctaacaaca accactgcgc ggcttgcgct ttatcgtcga tctcttccag caggcgttcg
    27601 tacagtggat tgtcggtggt ttcacgcaga caggcgatgc gcaactcgtg gatattgcta
    27661 cgtatacgac cgcgcaattc tgccgtggca gcctcggtaa aggtgaccac cagcagttct
    27721 tcaacggtca gcgggcgggg aaaggcggcg gaaccgccta gtccaagtaa caggcgcaaa
    27781 tagagcgccg caatcgtaaa ggttttgcct gtgccggcag aggcttcaat caggcgctcg
    27841 ccctgtaagg gcaagcgcaa aggatctagt gtctcggcga catcactcat tcttttcact
    27901 catcaggggc attgtttgct gcaacgcgct gacgttctcc cacactttcc agccttcagg
    27961 atgcacatat tcggctttcc cgttctggct gccggaaatc tgcgacagaa tagccatgcc
    28021 ttgcggctcg accaccgcct gatggaagaa atcagcaagt ttttgcggcg tcagcagttt
    28081 tatctgggcc acgattttat cacgcgaatc gaagcgcata ttgccgcgat cgaaatcttt
    28141 acttaacttc gatgcttctt cgccgagcgt ttgcggtgcc tgcagcatct gggtaattac
    28201 cgcctgctgg atttgcgcaa actcttctgg cttcatcgcc cgcaattttg cctctgcggt
    28261 tgggaaaaac gccttgtaac gctcccacaa gaatgaaggc tgtttatcat tgctttgcaa
    28321 aaggaagccc atgccccact gacgccccac gctcattgga aacgcaaaca cggcatagcc
    28381 caactgttct tcggtacgca actgattgta gaaccacggc tgtacgatct gccccaacag
    28441 tgagctatag gctgagctgg tgtattcatc gtagccagtc ggtacaaata ccgctgccag
    28501 tgcggagtcg gtgctgttac cggctttttc aaagatgacg gattgttttt tatcgaccac
    28561 gacatctttg ttacgacacc actccgaacc atcagcgccc aactgttttt gcacatcgcg
    28621 tgccagcgtt gttgcctggg cttcggtcat gttgccaata accataaact ctggtcgagc
    28681 ccctgatttt aaggcgtcgc gataggccag cacctctttc aacgtaatgg agggcaaaat
    28741 tttacgccgt tcatctcgcg agaagtacgg cacttgcgag agcatctgcg cgggcataat
    28801 cgcctgctca aacgctttac ccttttctgc ggaatccatc atctggttat accaggactt
    28861 cgcctgctca agctgatctt ccgtagcggt atagctaaag tacccctcga gcaatgcctg
    28921 gaacagctgc ggcagacgct gggtgtaacc attagcatta accataaggc cgttgttagc
    28981 gttggtggaa aaacttatgc caccaaccga cgcctggttg cttaactgat caagcgccag
    29041 ccctgcgaga taatcattga gcgcaaacat cacctgattg cgggcgctgt ccatggcttt
    29101 cggattacgc aaaatcaggc tgacatcagc tttgggttcg ctggaaaaat aacggcttgg
    29161 cgcatacacc acgcgcagat tcgactcatc aacaatcagc tctggatggt catatttctt
    29221 ctctgactta atcagcgaga aatcatcagg aatataaggg ttaagctctg gcaaagagag
    29281 cgcaatgtcg gcggcttttt tctgccagtc agcgaaagtt tgtgcgctaa ttttatcgac
    29341 ctgatacggc gcatcgacaa aataagccgt tttattgtgc ggctctttcg agctgatata
    29401 ccagatacgc gcattctgcg gcgtcatcat cgccagacgt tcctttactg ctttagcatc
    29461 gtaccgatcg gcaatattga ctgcatccag cgtatgctca acaggaacgc gaatcatggt
    29521 atctgccagc cattcgacgt aatccatatc acgggtgatc gacggataac ggaagtcgat
    29581 atccagcaca ttcgccagtt catcgaagta ttgtttatca atgccttttt cacgtaacag
    29641 attgagatag ctaaaaattg ccgccacaac ctgatcgcga ttagccaggc ctttatcggt
    29701 taaagacgca gagatcgcta atacgccgct gttgccgttg acgataggat cggagttggc
    29761 gctaatgccc tcaactaatc cctgcttttg cagccagtca gaaagtgtac ctgggctgcg
    29821 attgccaatc agataggtaa tcaattcatc ggttttacta cggaacttcg ctgagttgtt
    29881 atcgatgcga aactcaacgc gcaacacttt acgcggcagc gcagggacgt aatgaatgat
    29941 aatgcccttt tgcgcgtcgg tgactaccgg cacggtgatt tccggttttt tgctctcttt
    30001 gttcggcacg cgaccaaagg tgtccgccgc catttttgcc aactccggca gcggtttatt
    30061 actgtaaata accgccttca tcaaattggc ggaatagtac ttctcgtgga aatctttcag
    30121 cgcctgctgc accggattac caggtttgtc gcttaaagtt tcgaggttac caccagaaaa
    30181 ctttgaaccg gggtgtgccg ggttaatggt ttctgcgctg acctgtgcca tgcgcatccc
    30241 gtcacgcgta cgcgccatgg ttaattcggc attcaccgca ttacgctcac gttcggcgta
    30301 tttcttgtcg agcaagggtt ctgcaatagc gtccgccagg cggtctaccg caccgggcaa
    30361 ggcgtcgttc tcaacttcca gatagaaagc cgtgcgatac ggtgccgtgc tggcattgtg
    30421 gctgccaccg tgcattttga gatattcggc cagactgtca gcctgcgggt actttttcga
    30481 ccccatcaga ctcatatgtt caaggtaatg tgccagcccc tggtacgcct cgggatcttc
    30541 cagcgaccca acgggcacca ccagcgccga tagcgattta actgcctgcg gatcagaaac
    30601 cagcaagacc accataccgt tatccagacg tatagcctga tactggcggt tatctttatc
    30661 acttttacgg atggtttcct gaatcggctg ccatcccgtt tctgcctgac ttaagggtgc
    30721 ccaaagggca actaacaaca ataatgcttt gaaccaggtg ctgcggggca ttcacggacc
    30781 tcataatcaa cttaattttc tggccagatt caacaggtta acgcaaatca tctgccagaa
    30841 tttaatcttg tgctgcacga gtcagcctat gttatataac catcagtccg tgactggtgc
    30901 gcatcataaa gtaagcggat ggattgcgca atttttatac agcactcatg actgattaaa
    30961 gcgaaacagc ggtaacagga aacgttgcga ctgttcaacg atggcctcca ttgtctctgg
    31021 tgttaattgc cgccagagcc tttgatacca gatatcatca ccttcgccac gcaccatcat
    31081 gttgccttcg taagcctgaa ggaatttcgt acgggctttt tgcaacgtgg aatcgtcatc
    31141 cagcatggca tcgttttgcg cgtcataaca ggtttttagc cacgcgccgc cactttcagg
    31201 taacaccagc aatggcgcgg acatcccttc acgatacccc tcaatcagtt gtgagaggta
    31261 atgcaaagcc tgttcggctg caagcggcgg aaaacgccac tcgccgtctt tgcgtagaaa
    31321 aaggcgactt tcaccattac caccgctggc acagtagaca aggtgttcca gccaaagttg
    31381 cattccctgc gccacactta ataaagaggg acgccagcgc aacaggccat ccggctgcac
    31441 ctgcggcaac cagccagtta tctgcacacc gttgcaggcg agatcaattt ccatactctg
    31501 ccccggctgg cgacaggcaa tgactctgtc ggcaagctgc tgcatctcct ggcactgagt
    31561 ttcccagaaa atttcaccaa aagcgccata cggtaaatcc cctgccgccc ggaagcgacg
    31621 gaacaagcgt tcggcatcat cctgctcaac cagtgcattc aataactgct gattgatttg
    31681 ataacggcta agcccttcca gaataaatgg ctcggtgtcg gggatttcgc tgtcttcggt
    31741 acggaagttc acctgcaaac gcatctggaa aaatgcccgc accggatgtg cccagaatcg
    31801 ttgtagcgtt tccagcggca cggtttccgg taaggtaaac ggcagcggct gaacaaattc
    31861 agaatgtgct ttaccagcct ggctggccgc aggtagccat tcacgagcat agctttgtcg
    31921 ttcgcctggc tggtagtttt gtggatcaaa cggcatccgg gtatggaggc aagtaagatg
    31981 cgcttttacc cttgcctcgc tttcatcaca gttgagcgct tcatcgcccg gtagataatg
    32041 actttgcccg atgtagtcga tcagttcctg caccagtacc gacgggaaac gttcactgtt
    32101 atcctgaatg gaacgcccga tatagctgat atagagtttt tgctgcgcgg aaattaacgc
    32161 ttccaggaac aggtagcggt cgtcatcgcg acggctacgg tcgccacgct tcggtttctg
    32221 gctcatcagg tcaaagccca atggcgcaag ctgacgtgga taaacgccgt cgttcattcc
    32281 cagcaggcaa accactttga acggaattga acgcattggc atcagagtac aaatgttaac
    32341 cggtccggcg agaaaacgct ggctgatacg ttcttgatcc agacgctgtg ccagttcatc
    32401 acgcaatagt gacagcggca ccgcgtcgcc atactgcgca cctaaacctt cggcgataat
    32461 cgcctgccat tgttgttcga tcagcgtcat cgccgcttcg gtttccgcat ccggcaggaa
    32521 gaaggcgttg agcatatcgc gacaaaccgg caaccactct tccagcggac gctcctgcgc
    32581 cagcccgcga cgccagatgt ttagctgcat tagcagtgaa gccagatgcc ccaccagttc
    32641 tgcaattaag ccgctcgatt catcataagg tagaaccgat tgccactcgc cctgcgcgct
    32701 ctccatcgcg tagcccagca acatacgcgt caggccaaac cgccaggtgt gttgaccggt
    32761 ggcggggagt tccagctcgc gaacgttgtc gtcatctatc ccccaacgaa tgccggattc
    32821 gttgacccac tggcgtaaat aacgcagccc ttcttcggtg atgtcaaacc gcgccgccag
    32881 caccggcaca tccagcagcg ccagcacatc ctctgacaca aagcgactgt caggcagtga
    32941 taacaggctg ataaacgctt ccagtaccgg atgtgactgc cgcgcacgac ggtcggaaat
    33001 ggcgtaaggc aggtaacgat ccgcaggtgc gctaccaaac actgcctgaa taaacggact
    33061 gtagctgtcg atgtcggcca ccatcacgat gatgtcgcgc ggagtaagtg tcgggtcttc
    33121 ctccagcatc gccagcaggc gatcgtgcaa aacttcaact tcacgctgcg ggctatggca
    33181 aacgtggaag gtgatactgc tatccagtgg atcaagcggg cgtttgttat cgctacggga
    33241 aaactcttcg atgttcacac cagcaacggc gcggttttcc agttccagaa tgtcagactg
    33301 aatgttatgc agcaggttat ctggcgtgac atcgacaaaa gcgtccagct cctggctgct
    33361 ctccagatca gaaaggagat aaatgtagtc gcgcccaagc ttaccccagg aggccagtag
    33421 cgggttgccg acatcctgtt caccgtcgct gttaaagagc tgtccggcat tttcgctatc
    33481 gcgaaatagc ggcaattcgc gatcttcaaa actgtgtcga cgctgacggg tcagcagttt
    33541 cgccagataa gcaggatctt taatatcgcc ccagtaataa cggcaggggt tggtaaacag
    33601 gagatggatt tcaatatgtt tacccagcgc ctggagcgcc tggagataaa caggcggtaa
    33661 cgcggaaata ccgcatataa agacgcgcga aggtaacccc ggcgggcagg tcgtcgcgga
    33721 ctccagcgtt tcgataaagc gctgatagag attggcgcgg tgccaacgcg gttgtccgag
    33781 ttcatgtgta tattccacta gcgccttcca caacggcgct tgccaggcct gtgcctctcc
    33841 cagcccttca accaaatgtc ctgtttccca ctgtgccagc cagtccggac gatagaccag
    33901 atactggtca aacaggtccg ccgcttttga ggaaagctgg aacagctttc gcttgtcgct
    33961 atcgtcagtc agataatgcc gcaacagggt aaagtcttcg cgctccagca attgcggcag
    34021 cagagtcatc agtttccagc tcatgctctg tttgttaaag gcgctctctt tggggatctc
    34081 cggtaacacc cggacgaaca tatcccagat aaagctcgca ggcagcggaa aatcaatgtt
    34141 tgccgcaata ccaaactttt gcgacagggt catttgcagc cactgtgcca taccggtact
    34201 ttgcaccaga atcatctctg gttcgaaagg atcgtccagc cgttcgcgtt cgacaataaa
    34261 ctccatcaac gcttccagca cgtccagacg attggaatgg tagaccctta acatagcggc
    34321 tcctgactac tgacgattcg ggcaatgcag gcgggtcatc tcgccttctc tgcccccggg
    34381 tgaaactagc gtaacgctga tgctgacaca tcccgcctgc gatgtctgca ttcggttgac
    34441 ctgccagttg gcaggtggcg aaatcgcgcg cagttgcgtt tgctgccaac catgccgcca
    34501 gagctgttgg tactggtttc tgctggcaag actgttcatt aatgttcgct ggaaacccga
    34561 taacgcagtg acaatcatca ccatcagcac catcgccaac attacctccg gcaggctaaa
    34621 gccttgttga ttcttcaggg aagctgacat aacgccccct ctttcagcgg acaaaaatcg
    34681 ctccagccgc gtggcgaaaa accaatgttt ccatcgatga cttcacctgt tcgccacaac
    34741 gaaacgcctt cataaccggc aatcaataag gcttcattat ctgccaataa acgcaaacaa
    34801 acacgggcat cggttccagc gtactgcgag cactgaactg ctggctgcgt ctgccaggag
    34861 tgcatttttc cccacgccag cgccgactga acgatggcct ggcggcgcaa tgactgactt
    34921 tccatgctca cgcgagaggc aaaactgcga tcctgttggc tcattccttg taatagcaag
    34981 ctacccaaaa ccagcagcat caggaccaga gccagtgacg aaacaccctt ttcgcggttc
    35041 acaggttgaa tcctgtcacg ctatagctgg cattcaccac ggtttgcggt tcagacttac
    35101 tggcagcacg catattaacc gtcaacaccg gcgagaagcc gctgacatcc tgacgtacga
    35161 cctgaaaagt gtcgataatg atggcatccg gattagtgac tttatcccag cccttacctt
    35221 cacaggatgt cgcaccgcgt agcgtttcca gcacatgctc cttcagacga aatccaatct
    35281 ggtcggactc ttttaccggt tcgcgatccc agataccgtt actgttcgca tcccactgca
    35341 caatgataca gtcaccctgt ccgacaattt ccagcccttc gccggtacag ctgccatgac
    35401 aataacccgc cctctggaga tgcttcgcga cggtaaatac ccgcagccag atttcatctt
    35461 ccagcgccag cttacgggtg ctcgttaaac tttcacgctg taacgcaggc agaaagcgtg
    35521 cagcccccag caacaatacg ctactgatcg ccatagcaat caacacttcc agcagagaaa
    35581 aaccttgctc tttcacaggc atccttctgt ttctccttgc tgacaaagcc ggagtcttcc
    35641 ccacggcgaa accaccagcc accactcgcc cgttgagttt ttgaagcgaa tatgcccggc
    35701 ccatgcggta ttgcgtaggc caaagaaagc aagcgaaggt gtcaggtcgc tcatttcgac
    35761 ttcgggccag cgtggcacaa agaccaatgg tgaactgcca tgacaggtat tggccccagc
    35821 agcggaactc acaaggcacc ataacgtccc ctccctgata acactgatac tgtggtcgcg
    35881 gttatgccag ttggcatctt cacgtaaata gagcaaatag tcccgcgcct ggctggcggt
    35941 ttgccaaagc cgttgcgact gctgccagta ttgccagcca tagagtccac ttgcgcttag
    36001 catgaccaaa atcagcatcg cgaccagcgt ttcaatcagc gtataaccac gttgtgtttt
    36061 catgccggca gtatggagcg aggagaaaaa aagacgaggg ccagtttcta tttcttcggc
    36121 gcatcttccg gactatttac gccgttgcag gacgttgcaa aatttcggga aggcgtctcg
    36181 aagaatttaa cggagggtaa aaaaaaccga cgcacactgg cgtcggctct ggcaggatgt
    36241 ttcgtaatta gatagccacc ggcgctttaa tgcccggatg tggatcgtag ccttcaatct
    36301 caaagtcttc gaaacggtag tcgaagatgg attcgggttt acgtttgata atcaacttcg
    36361 gcagcggacg cggttcgcgg cttaattgca gatgagtttg atccatatga ttgctgtaca
    36421 ggtgcgtgtc gccccccgtc cagacaaaat cgcccacttc cagatcacac tgctgcgcca
    36481 tcatatgcac cagtaacgcg tagctggcaa tgttaaacgg caggccgagg aaaacgtcac
    36541 aggagcgctg ataaagctgg caagagagtt tgccgtctgc cacatagaac tggaagaacg
    36601 catggcacgg tgccagcgcc attttatcca gttcgcctac gttccacgct gaaacaataa
    36661 tgcggcgcga atccgggtcg tttttcagct ggttcagtac cgtagtgatc tggtcaatat
    36721 gacgaccatc cggcgttggc caggcgcgcc actgtttacc atacactggc ccgaggtcgc
    36781 cgttttcatc ggcccattcg tcccagatgg tgacattgtt ttcgtgtaga taagcaatgt
    36841 tagtgtcgcc ctgcagaaac cacagcagtt catggatgat ggaacgcagg tggcaacgtt
    36901 tagttgtcac cagcgggaat ccatcctgca ggttaaaacg catctgatga ccaaaaatgg
    36961 aaagcgttcc ggttccggta cggtcgtttt tctgtgtgcc ttcgtcgagc actttttgca
    37021 tcagttctaa atactgtttc atggttcctc aggaaacgtg ttgctgtggg ctgcgacgat
    37081 atgcccagac catcatgatc acacccgcga caatcatcgg gatggaaaga atttgtccca
    37141 tgctgatgta ctgcacccag gcaccagtaa actgcgcgtc gggctggcgg aaaaactcaa
    37201 caatgatgcg aaacgcgccg taaccaatca ggaacaaacc tgagacagct cccattgggc
    37261 gcggtttacg aatatacagg ttgaggataa taaacagcac tacaccttcc agcaacagct
    37321 cgtaaagctg tgatgggtgg cgcggcagca caccgtaagt atcgaaaatg gattgccact
    37381 gcgggttggt ttgcagcagc aaaatatctt ctgtacggga gccagggaac agcatggcaa
    37441 acgggaagtt cgggtcaacg cggccccaca attcaccgtt aataaagttg cccagacgcc
    37501 cggcaccaag accaaacgga atgagtggtg cgataaaatc agagacctgg aagaaggaac
    37561 gtttagtacg gcgggcgaag ataatcatca ccacgataac gccaatcagg ccgccgtgga
    37621 aagacatgcc gccgtcccag acacggaaca gatacagcgg atcggccata aactgcggga
    37681 aattgtagaa cagaacataa ccaatacgtc caccgaggaa gacgccgagg aagcccgcat
    37741 agagtaagtt ttcaacttca tttttggtcc agccactgcc cggacgattc gcccgtcgtg
    37801 ttgccagcca cattgcaaaa atgaaaccca ccagatacat caggccgtac cagtgaagcg
    37861 ccacgggtcc tattgagaaa atgaccggat caaactccgg aaaatgcaga tagctactgg
    37921 tcatctgtca ccacaagttc ttgttatttc gctgaaagag aacagcgatt gaaatgcgcg
    37981 ccgcaggttt caggcgctcc aaaggtgcga ataatagcac aaggggacct ggctggttgc
    38041 cggataccgt taaaagatat gtatatgatc cgcgctataa ccctccgcga atcagcccgc
    38101 ccatgccgcg acgctccata aaggctgcaa cctgatggcg aacttcggtc gccagttgcg
    38161 cttccagact acgctgcgca agattttctg cgtcggcata atcaatgcgc cgcagcaggt
    38221 attttacccg cgctacagaa cgtccgttca tagacagatg gcgataccca agcccaatga
    38281 ggattgccac gcacatggga tcgcccgcca tttcaccgca caaacggaga tcgattccat
    38341 gtatttccgc ttcccgggcg atcatcgcca gagctcgtaa cattgcagga tgaagactgt
    38401 cataaatgtt cgccacccgg gtattgttgc gatcaacggc cagaatgtat tgagtcagat
    38461 cgttggtgcc aacagagatg aaatcgaccc gctttgccag atgcggcagc ataaatacca
    38521 ttgacggcac ttccagcatg atgccgatac gtggtttggg aatttcgtaa ccgatcatct
    38581 cctcgacttc acgtccggca cgttcaatca ggcggcgtgc ttcatcaact tcatcgaggc
    38641 ttgtgaccat cggcaacaga atattcaggt tgcccgtagc ggcattagca cgcagcatcg
    38701 cccgcacctg gatcaagaag atctccggct gatcgagcgt aatgcgaatc ccacgccaac
    38761 ccaggcatgg attctcttcg ctgatcggca tgtaaggcag ctgcttatct gctccgacat
    38821 ccagcgtacg caaggtgacg ggtttatcat tgaacatttg cagcatcccc tgatactgcg
    38881 ccacctgttc ttcttccgac gggaaaccac tttgcagcat gaatgggatt tcagtgcgat
    38941 aaagaccgat gccatcaata cggctgccca gtttttcttc atgttccggg cttaaaccag
    39001 cattgagcat gacttttata cgctcaccgc tttttaactg cgcgggtaaa ttgacgtcat
    39061 cttccgccag acggctaagc tcaatctctt cactaattag ccgctgatat tcttgcagca
    39121 gtaccggctc cggatcgacc agcaattcac cgcgatagcc atcaacgatc agcgtcctac
    39181 gatgcagcac cgaaggctga atatccgcgc ccatcacggt agggatcccc agcgcacgta
    39241 ccatgatcgc agcatgggag ttggctgcgc catctcgcac gacaacaccg actaagcgat
    39301 cctggggcag ctcagcaaga gttgtcgctg acagttcatc tgccaccaga atgaaacgtt
    39361 ccggccaggc gttcggccct tgattagcgt catcaagatg aaacagcaat cgctgaccca
    39421 gcgcacgtaa atcgccagcc cgctctttga gatagttatc gcttagcgcg gcaaactgtt
    39481 cggcaaattt ttcaatgacc gtttttaccg cccactctgc caccgagcct ttatcaacct
    39541 cggcaaacaa ttcgcgacgc agccgggtgt ccgaaagtag gtgcgagtaa agatcgaaaa
    39601 tagccgccgt ttctttttgt gctccggcgg caaagcgttt gctgtagcgg cgaaactcgt
    39661 ttgccgcttc ttccagcgcc ccggtcagtc gttcgcgttc cagagccgga tccagcgttg
    39721 atgcctgata cacctgttcc attaaaggta acgtggcatc ctgccagcct tcggcaatcg
    39781 ccacaccagg tgctgccggt aatgcgcgga ttcgcgtctg gcgatattgc ccaaacaggg
    39841 cagtcaactg cgactgggaa agaatagctg ccatctgggt ggcaagcgtc accaggaagg
    39901 attcttcact ttcgtcatac tggcgcaact ctcgttgctg taccaccagt acaccaagca
    39961 actggcgacg ttgaataatt ggtacgccta aaaacgcgcg gaaacgttct tcttttacgg
    40021 aggggatgta tttgaagctg gggtgctttt gcgcatctgc aaggtttatc ggttccgcca
    40081 gcctgccaac caggccgacg atcccttcat caaacgcgag cgttacagtg cgaccgcgtg
    40141 gttttttcag cccccgggtc gccatcaggt agtaacaacg tcgatcatga tcggccaggt
    40201 agaccgaaca gacctcggta tccatcgcaa gacagatgtc ggtaaccaga atatttaacg
    40261 cctcattcag gcgtggtgcg ctggctacct tttcgactat ttcgcgcagg cgagtgagca
    40321 taattggggt gacttaacct cttttacgtc gataagcaga tgcgttttgt ggttttggcg
    40381 tattttcctg cagtgacatc accacactcg cgaactcttt cattacccta cggtagacat
    40441 cacgtttaaa tgacaccacc tgtctgaccg gataccagta acttacccat cgccagccgt
    40501 caaactctgg tgtactgctg gtttgcatat tgatttctgc atcgccgctc accagctgca
    40561 agagaaacca tttttgtttt tggccgatac aaaccggctt cgtgtcccaa cgcaccaaac
    40621 gtttcggtaa tttgtagcgc aaccagttac gcgttgaagc aaggattcga acgtctttgc
    40681 ggcttaatcc tacttcttca aacaattcac ggtacatcgc ctgctctgcg gattctccgg
    40741 ggttgattcc gccttgcgga aattgccagg agtgctgacc aaatcgccgg gcccacatta
    40801 cctgcccctg gcgattacaa atcacgatac ctacgtttgg gcggtagcca tcgtcatcaa
    40861 tcaccggact acctcaaaat aaagctttat atacgaatga ttgtttcata ctccaggaag
    40921 acggtaaacc actctctgca gggcattaca cactaataac aattgaataa ctcacagtta
    40981 tgtgcagagt tataaacaga ggaaggggtg gatagccgtt tttattcacc ttttctgtgg
    41041 atagagttgt gaagaactac ggaattacta cgggaaaac
//
LOCUS       DAPGOF010000019        40574 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.019, whole genome shotgun sequence.
ACCESSION   DAPGOF010000019 DAPGOF010000000
VERSION     DAPGOF010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40574
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.019"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(80..793)
                     /gene="lpxT"
                     /locus_tag="RHV13_000900"
     CDS             complement(80..793)
                     /gene="lpxT"
                     /locus_tag="RHV13_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HDV1240455.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFADINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            complement(832..1818)
                     /gene="yeiR"
                     /locus_tag="RHV13_000901"
     CDS             complement(832..1818)
                     /gene="yeiR"
                     /locus_tag="RHV13_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HDV1240456.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGHQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            complement(1936..3402)
                     /locus_tag="RHV13_000902"
     CDS             complement(1936..3402)
                     /locus_tag="RHV13_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDV1240457.1"
                     /translation="MNTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQAIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATAESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLDE
                     QAPTLRIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIAQFGAHQALLNTLKI"
     gene            complement(3625..4197)
                     /gene="yeiP"
                     /locus_tag="RHV13_000903"
     CDS             complement(3625..4197)
                     /gene="yeiP"
                     /locus_tag="RHV13_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HDV1240458.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            4352..4606
                     /locus_tag="RHV13_000904"
     CDS             4352..4606
                     /locus_tag="RHV13_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HDV1240459.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            complement(4603..5784)
                     /gene="setB"
                     /locus_tag="RHV13_000905"
     CDS             complement(4603..5784)
                     /gene="setB"
                     /locus_tag="RHV13_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HDV1240460.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFIGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTIEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            6152..7282
                     /gene="fruB"
                     /locus_tag="RHV13_000906"
     CDS             6152..7282
                     /gene="fruB"
                     /locus_tag="RHV13_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HDV1240461.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            7282..8220
                     /gene="fruK"
                     /locus_tag="RHV13_000907"
     CDS             7282..8220
                     /gene="fruK"
                     /locus_tag="RHV13_000907"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDV1240462.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            8237..9928
                     /gene="fruA"
                     /locus_tag="RHV13_000908"
     CDS             8237..9928
                     /gene="fruA"
                     /locus_tag="RHV13_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HDV1240463.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            10352..11293
                     /locus_tag="RHV13_000909"
     CDS             10352..11293
                     /locus_tag="RHV13_000909"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HDV1240464.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            11281..12219
                     /gene="psuG"
                     /locus_tag="RHV13_000910"
     CDS             11281..12219
                     /gene="psuG"
                     /locus_tag="RHV13_000910"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HDV1240465.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     ARTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEHTINAAIDQAVAEAEAQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            12313..13563
                     /locus_tag="RHV13_000911"
     CDS             12313..13563
                     /locus_tag="RHV13_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDV1240466.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFSFTNASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(13631..14329)
                     /gene="yeiL"
                     /locus_tag="RHV13_000912"
     CDS             complement(13631..14329)
                     /gene="yeiL"
                     /locus_tag="RHV13_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HDV1240467.1"
                     /translation="MKEIHNNDLKLQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVSLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            14459..15400
                     /gene="rihB"
                     /locus_tag="RHV13_000913"
     CDS             14459..15400
                     /gene="rihB"
                     /locus_tag="RHV13_000913"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HDV1240468.1"
                     /translation="MEKRKIILDCDPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            15500..16750
                     /locus_tag="RHV13_000914"
     CDS             15500..16750
                     /locus_tag="RHV13_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDV1240469.1"
                     /translation="MDVMRSVLGMVVLLTIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTADTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(16806..17894)
                     /locus_tag="RHV13_000915"
     CDS             complement(16806..17894)
                     /locus_tag="RHV13_000915"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDV1240470.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGSVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLAGEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMM"
     gene            complement(17897..18754)
                     /gene="nfo"
                     /locus_tag="RHV13_000916"
     CDS             complement(17897..18754)
                     /gene="nfo"
                     /locus_tag="RHV13_000916"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDV1240471.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDKFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(18828..19877)
                     /locus_tag="RHV13_000917"
     CDS             complement(18828..19877)
                     /locus_tag="RHV13_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HDV1240472.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            19976..20857
                     /locus_tag="RHV13_000918"
     CDS             19976..20857
                     /locus_tag="RHV13_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV1240473.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            21062..22531
                     /gene="lysP"
                     /locus_tag="RHV13_000919"
     CDS             21062..22531
                     /gene="lysP"
                     /locus_tag="RHV13_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HDV1240474.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPGKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            22823..24802
                     /gene="cirA"
                     /locus_tag="RHV13_000920"
     CDS             22823..24802
                     /gene="cirA"
                     /locus_tag="RHV13_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HDV1240475.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFTA
                     NGVPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLSDLPF
                     HTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVTKDVK
                     LRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(24834..25670)
                     /gene="yeiG"
                     /locus_tag="RHV13_000921"
     CDS             complement(24834..25670)
                     /gene="yeiG"
                     /locus_tag="RHV13_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HDV1240476.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSRYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            25928..26596
                     /gene="folE"
                     /locus_tag="RHV13_000922"
     CDS             25928..26596
                     /gene="folE"
                     /locus_tag="RHV13_000922"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HDV1240477.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            26613..27770
                     /locus_tag="RHV13_000923"
     CDS             26613..27770
                     /locus_tag="RHV13_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HDV1240478.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVDAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMQFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGPAISKTSR"
     gene            27912..28952
                     /gene="galS"
                     /locus_tag="RHV13_000924"
     CDS             27912..28952
                     /gene="galS"
                     /locus_tag="RHV13_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HDV1240479.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            29232..30230
                     /gene="mglB"
                     /locus_tag="RHV13_000925"
     CDS             29232..30230
                     /gene="mglB"
                     /locus_tag="RHV13_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HDV1240480.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWTANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            30291..31811
                     /gene="mglA"
                     /locus_tag="RHV13_000926"
     CDS             30291..31811
                     /gene="mglA"
                     /locus_tag="RHV13_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HDV1240481.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            31827..32837
                     /gene="mglC"
                     /locus_tag="RHV13_000927"
     CDS             31827..32837
                     /gene="mglC"
                     /locus_tag="RHV13_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HDV1240482.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(33080..34315)
                     /gene="preA"
                     /locus_tag="RHV13_000928"
     CDS             complement(33080..34315)
                     /gene="preA"
                     /locus_tag="RHV13_000928"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HDV1240483.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(34309..35547)
                     /gene="preT"
                     /locus_tag="RHV13_000929"
     CDS             complement(34309..35547)
                     /gene="preT"
                     /locus_tag="RHV13_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HDV1240484.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKMLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDVFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(35741..35980)
                     /locus_tag="RHV13_000930"
     CDS             complement(35741..35980)
                     /locus_tag="RHV13_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HDV1240485.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(35983..36702)
                     /gene="sanA"
                     /locus_tag="RHV13_000931"
     CDS             complement(35983..36702)
                     /gene="sanA"
                     /locus_tag="RHV13_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HDV1240486.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(36852..37736)
                     /gene="cdd"
                     /locus_tag="RHV13_000932"
     CDS             complement(36852..37736)
                     /gene="cdd"
                     /locus_tag="RHV13_000932"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDV1240487.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHELRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(37866..38561)
                     /locus_tag="RHV13_000933"
     CDS             complement(37866..38561)
                     /locus_tag="RHV13_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HDV1240488.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(38558..38956)
                     /locus_tag="RHV13_000934"
     CDS             complement(38558..38956)
                     /locus_tag="RHV13_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDV1240489.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAI
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            39195..40145
                     /gene="dusC"
                     /locus_tag="RHV13_000935"
     CDS             39195..40145
                     /gene="dusC"
                     /locus_tag="RHV13_000935"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HDV1240490.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQQLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LGYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIDKLR"
BASE COUNT         9975 a         9989 c        10645 g         9965 t
ORIGIN      
        1 cgatggatga aagcacggtg cgcatcatca ggattatcct cactataaaa ataaacctga
       61 tgatgttaat tactgtgagt tatttgtttt ggaaatgttt gttttttccc ggtagtgatt
      121 tgtcaaaaaa ggtgattaat cgatcactta atggcgtcag caacacccag ggcaaaccga
      181 tcaatatcac ggtcatcgaa ccgacaatga tgtcagtaaa ccagtgtgcg ccaatcatta
      241 ctctgggaaa cgcaaaaacc acaaaaataa taagggcgat aagacctgca actttgccga
      301 aataacgcca catgaatgcc gaaaaaataa gcagcatcat gccgtgatcg ccgggaaagc
      361 tatctcgtga ggcatctttc gtggggacag agagcagttc gctgacgcgg ttaatatcgg
      421 caaaagtcaa tgttgggctg gcccgtttta caggaattaa tgcctgaccc agctggttta
      481 ataccactgc agttaatagc atgaccagac caataatcac gatacgtcgt ctgcctgggg
      541 cgttttcttt cagccagaaa ctcagcatca acattcccat cgccagcagt gaacaaccgt
      601 cgaaagcgcg attgttggtc aatgcaacca gccataaaaa ggccttactt tcgaccagtt
      661 tctgattaaa gaaataaaaa atatccgcat caatcggcaa ccagaaccca tgattaacgg
      721 gaatatacca ggaaagaaac agcgcgaggc cgacaatatt caacaacact atttgcggca
      781 aatttttaat catattttct ctaattatct tactgaaaaa cgcaggcaac cttacgcggt
      841 agtcgctaaa cgaagcttca acagcgcgct ctgcaaggca ttccagtcag cttcgctgct
      901 ggaaatcagc tcaatacggc tgtccggtgg cgcaacgttt tgcgtttcaa tgtgcaggtc
      961 atcgccctga cggttgattc gcaccagccc ttcgggaata cgcagcacgc ctttgacgcg
     1021 ttccaccggt gcaagtcgcg cccattccag aatgccaatg gtgtcgaata ccgtatcagc
     1081 gtcgaatatc cagccgcagg cctgatgtcc ttgtccactg ttcagactgc gacgccagcg
     1141 ttgatgctct ggcaggctta acgctgctaa cccttttttc acgacatgct gatgagaatg
     1201 cgcggcgctg gcgggcaact cggctaaatt gcgacgcggc aaatccagaa gatgaccgtc
     1261 aactttccca tgctcactgt gaattaattg ccgatcgcca ccattttgct gccaccagcg
     1321 ctgtagcgct tgctcgcttt caggtgtcgc acggtcggat ttattggcga caatgatgtc
     1381 tgcggcagcc agctggtcac ggaagttttc attgctggcg cttttttcgt ccagcagcag
     1441 gcgtggatcg agaatgcaca acgtggcgcg tagatctatc cacggttcat agactggtgc
     1501 ggttaagaga tcgaggatct gtttcggatg gcccagcccg gtcggctcta tcaacaagcg
     1561 gtctggtttt ccctgacgca gtaaggtatt caaccctacc tgcatgggta aaccattaac
     1621 gcagcacatg cagccgccgg ggatctcttt cagcagtgcg ccgctatcgg cgagcaaagc
     1681 accatcaatt ccgacttccc caaattcatt aaccagtacc gcccattttt cgttgggatc
     1741 tttatgggct aacagatgaa gaatcgacgt agttttcccg ctgccgagaa aaccggtgat
     1801 gaggttggtc ctggtcacaa cggctccgca atactctggt aagttaactg agcgttaatc
     1861 ctggctaaaa acggcagaaa agagaagggg agagcggacg aaggcgagct tcatccgctt
     1921 taaccgcaga aatcgttaaa ttttgagggt gtttaatagc gcctgatgcg cgccgaattg
     1981 tgcgatttgt tgccaggctt gttcgatggc ctgcacaaaa tgcgggttat ctggcagatc
     2041 atcaccgaaa acttcacgca gggaaagcag ggcggtgacg cgttgttcac tgctgctgcc
     2101 cgcaacaagt tcgcgaattt tatcgctaag cggatcgcga acatcaatgg catttccggc
     2161 atcatcaacg ccgctgacgt aacgcatcca gcctgcaacg cctaatgcca gcaacgacca
     2221 gtccgtttcg cgccccagat gtatgcgaat acctgccagc atgcgttgcg gtaatttctg
     2281 gctgccatcc atcgcgattt gccaggtctt atgtttcagc gccggattag caaaacgtgc
     2341 aattaactta tccgcatatt gtgttaaatc gacatcttta atccgcagtg tcggcgcttg
     2401 ctcatccagc attaatgttc tggcggcatg gcgaaatgcg cgatcctgca tgcaatcact
     2461 gatatgggcg aatcctgaga ggtaacccag ataagcgaga aaagagtggc tgccattaag
     2521 catccgcagt ttcatctctt cccatggcag gacatcattc accatttgta cacctgcgac
     2581 ttcccaggca ggacgcccag cgacgaagtt atcttccacc acccactgga taaacggttc
     2641 gcagctaatc gcgcagggat catttacccc cagatgctgg ctgatttctg ccagtgattc
     2701 ggcggttgca gccggaacaa tgcggtcgac catggttccc ggaaaactga cgtgttcttt
     2761 tatccacccg gcgagttctg gcgaacgttt ttctgccatt cccagcaccg cgtttttcac
     2821 cacatggcca ttgtcgggaa tattgtcgca ggagagcacg gtaaacggtg taaggccgcg
     2881 ctcacggcgg cgtttcagtg cttcgacgag aatacctggc gcggagtgag gttcttcagg
     2941 ggtttgtaga tcgtgaataa tccgcggatt gctggtgtcg agtgcaccgg tggccgggtc
     3001 aatacaatag cctttttcgg taatcgtcag ggaaacaatt gccacctgtg gctcgcaaaa
     3061 tttctcaata attgccgcta aggaatccag ttttgcattc aggcattcgt gaacggcacc
     3121 gacgattatc gcctgattgc cgtccgcccc tttctccagc acggtatata aatggttctg
     3181 tgcgcggagc tggctcatca gttgatcacc gctgaacaag ctgatttcac agatccccca
     3241 gtcgccgccc tgggcattca gcacacgatc ggtcagtaac gcctgatgtg cgcggtgaaa
     3301 ggctccaaag ccaaaatgaa cgatacgtga ttgcaactgc tggcgatcgt agcgtggagc
     3361 gtgtacatga tgcgggagcg taacggaggc gattgtattc ataggagtca cgtcttagta
     3421 acgattaatt aacaatgaca gtgtaaagtt atatgacagc aattgaaatt ggtctggatt
     3481 gttaatgcga tgaagtgtga gggcgatcaa tcaacgagtg aaaattgcgg gcataaggtg
     3541 ttcaggaagc gcaaaataga agattggtat aataaattaa aaaagggaca gcctgagcta
     3601 tcccttttct gcatgcggct gaagttagtc agcacgcccc atataacggc gttcttcgat
     3661 atggatacga attttttcgc ccgggcttaa gtattctggt acctgaatca ccagaccagt
     3721 gctcaatgtc gccggtttgt tacgggcgct ggcagatgcc cctttgatgc ctggtgcggt
     3781 ttcaacgatt tccagatcaa cggtctgcgg cagctcaagc gccagcagtt gaccgtccca
     3841 ggtcagcacc tgcatgtccg gcatgccgcc ttctggcata aacagcaact cttcttcaat
     3901 ctgatctttg gtgaaggtat acggggtata gtcttcttta tccataaaga catattcgtt
     3961 gccatcgaca taggagaagt caacgtaacg gcgggtcagc gtcacggtgt caacgatatc
     4021 atctcctttg aaacgctctt ctactttcag cccggtacgg acatcagaaa aacgcatttt
     4081 gtacagcgta gcggcaccgc gggcagtggg cgactgaata tcaatgtcct taaccagcag
     4141 cagtttgccg ttgtaattca gtaccatacc ttttttaatt tcgttcgctc ttggcatcga
     4201 aaaattcctg tagtcgaaaa tgtcaaaaat atcgcgacaa agtactcgcg acgggccttt
     4261 cgggcaagtg gtattcgcac ttttgctggt gcaaaaaagg tggtactgtg cgcgctcatc
     4321 aatccggtgg ttaaccttaa gagaacaacg tatggaatgc cgtccgggtt gcggggcgtg
     4381 ttgcactgcc ccttcaattt ccagccctat tcccggtatg ccagatggca agcccgccaa
     4441 tacgccctgc attcagcttg atgaacagca acgctgtaaa attttcacct cgccactgcg
     4501 gccgaaagtc tgtgcaggct tacaagccag cgctgaaatg tgcggtaact cgcgtcagca
     4561 ggcaatgacc tggcttatcg atctggaaat gctgaccgcg ccttaaacat ctttaatccg
     4621 cagtaagcaa aacagagtgg cgataatcat caccatcgca aaccagaaca cagcgtgata
     4681 attccagatc tcggcgacga tgcccgctac agatcctgcg atgatccagc ccacgcgcga
     4741 cgtgttggta tagagcgtgg tggctgaacc tgcctgaccg ggcatcaaat cctgaaaata
     4801 gagcatcccg atgccgccca gaatgccaat aaaaatagca tttagcagct gcaagcccaa
     4861 cagaatggca ggtgaatgcg ccatcagcat tcctgcgtaa aaacagacgc cacccacggc
     4921 agcaacgcgc attaagaaac gcttacccag acgtttggcg aaatatccgg caatcaacat
     4981 cgtcgggatt tccagcccgg cggcggtccc catcatcaca ccggccagtt tctcgggaag
     5041 atgcagttcg ttgataataa atagcggcat gttgatgatg tacaggctgt tcgagcccca
     5101 catcaatgta caaatgacaa acagcagcag cgtatcgcga cggttacggc gcggcgcttc
     5161 gatcgtgccg gtcgccagcg gaagctcttt ttgcatcgac ggtaaaaaca gccacaccat
     5221 cacaccgcaa acaataaacg ctaccgctgc gctcagatac attaccgtaa agctgaaacc
     5281 catcgctaag gcataagcca gcggtgggcc aatgacccat gccagtgaaa cctgagcgcg
     5341 taaaaaagag ctgaacatca ccgcctcacg tccggttttg tcggcatgtt cacgggcaag
     5401 ggcaaacatt tgcgggttag cggtcgagcc aaagctgcta agaaagacgc caatgaatag
     5461 caaaacaaag tagttgcgat tccaggcaaa aagggtgcag gccagcacgc ctaacaggca
     5521 gcaaaagaca atcagcgatt tgcgatcgcc gcgcttatca gagcgcccgg cgagaaactg
     5581 actcaccaga attccaatga cagcgctgcc ggtaaagaag aatcccacca tcgccggacg
     5641 ggcatgtact tcatcggtaa gaaaaatact gagtgtcggg gtttgcagag cgcccgcaat
     5701 accggtgaga aaggcaacga ttaaaaacgc cgtcgaggtc aggtcaaacg atttcgcgct
     5761 ggagactgcg ggggagttat gcatgctggt cgatcactgg aagaggaaga cgcggagttt
     5821 acgctgcttg tcagaaaata aacagacagc gaaatcaaaa gcgccacgaa taatcaaaat
     5881 gctatttcac tgttgctgat tggctgctga cgctgctgaa atgaaattgc tgataacgga
     5941 ttttcccatc agcaattagg aaaaatgaca aaaaattgtg cagcacatca aacttttgct
     6001 cataacttta cggctttcct tgcgtgctga aacagaagta ttatgctttc ttgaaacgtt
     6061 tcagcgcgat cttgtcttta accctaagcc aggttggcgc tttttttctc atagaagctg
     6121 aatcgtttca attcagcaag agaggagaac tatgttccag ttatccgtac aggacatcca
     6181 tccgggcgaa aaggccggag acaaagaaga ggcgattcgc caggtcgctg cggcgctggt
     6241 gcaggccggt aatgtagcag aaggctacgt caatggcatg ctggcgcgcg aacagcaaac
     6301 ctcaacgttc ctcggcaatg gtattgctat tccacacggc actaccgaca cccgcgatca
     6361 ggtgctgaaa accggcgttc aggtatttca gttcccggaa ggcgtcactt ggggtgacgg
     6421 tcaggtagcg tacgtagcga tcggtattgc tgccagctcg gatgagcatc tgggcctgct
     6481 acgccagctg acccacgtac tgagcgatga ttccgttgct gaacaactga agtcagcaac
     6541 aacagcagaa gaacttcgcg cattgctgat gggcgaaaag cagagtgagc agctgaagct
     6601 cgacaacgaa atgctgacgc tggatatcgt cgccagcgat ctgctgactc ttcaggcgct
     6661 gaacgctgcg cgtctgaaag aagcgggggc agttgacgcc actttcgtca ccaaagccat
     6721 caatgaacaa ccgctgaacc tcggacaggg catctggctg agcgatagcg ccgaaggcaa
     6781 tttgcgtagc gcgattgctg taagccgtgc ggcaaatgct tttgatgtgg acggcgaaac
     6841 ggcagccatg ctggtgagtg tggcgatgaa tgacgatcag cccattgcgg ttcttaagcg
     6901 tctcgctgat ttgttgctcg acaataaagc tgaccgcttg ctgaaagcgg atgcggcaac
     6961 gttgctggcg ctgctgacca gcgatgatgc gccgaccgac gacgtgttaa gcgcggagtt
     7021 tgtggtgcgc aatgaacatg gcctgcatgc tcgtccaggt accatgctgg tcaacaccat
     7081 taaacaattt aacagtgata ttaccgtgac aaaccttgat ggcaccggca aaccggcaaa
     7141 cggacgtagt ctgatgaaag ttgtggcact tggcgttaag aaaggtcatc gcctacgctt
     7201 taccgcccag ggtgcagatg ctgaacaggc gctgaaagca atcggcgacg ctatcgctgc
     7261 tggtcttggg gagggcgcat aatgagcaga cgtgttgcta ctatcaccct taatccggct
     7321 tatgaccttg ttggtttctg cccggaaatt gaacgcggcg aagtgaacct ggtgaaaacc
     7381 accggtctgc atgcggcggg taaaggcatc aacgtggcca aagtattaaa agacctggga
     7441 attgatgtca ccgttggcgg cttcctgggt aaagacaatc aggatggttt tcagcaactg
     7501 ttcagcgagc tgggcattgc caaccgtttc caggttgtac aggggcgcac ccgaattaac
     7561 gttaagctga cggaaaaaga cggcgaagtg accgacttca acttctcggg ttttgaagtc
     7621 acccccgccg actgggaacg ctttgtgact gattctctga gctggctcgg tcagttcgat
     7681 atggtctgtg tcagcggaag cttaccgtca ggcgtcagcc cggaagcgtt caccgactgg
     7741 atgactcgcc tgcgtagtca gtgtccttgc attatctttg atagtagccg tgaagcgtta
     7801 gtagcaggtt tgaaagcggc accgtggctg gtgaaaccta accgccgcga gttggaaatc
     7861 tgggcaggcc gtaaactgcc tgaaatgaaa gatgtgattg aagctgcaca tgcgctacgt
     7921 gaacaaggta ttgcgcatgt tgttatttca ctgggtgccg aaggcgcgct ttgggttaat
     7981 gcctccggcg aatggatcgc caaaccaccg tcagtcgatg tcgtaagcac cgttggcgca
     8041 ggggattcta tggttggtgg cctgatttat ggcttgctga tgcgtgaatc cagtgaacac
     8101 acactgcgtc tggcgacagc tgttgcagcc ctggcggtaa gtcaaagcaa tgtgggtatt
     8161 accgatcgtc cgcagttggc cgcaatgatg gcgcgcgtcg acttacaacc ttttaactga
     8221 cagcaggaga ggcataatga aaacgctgct gattattgac gctaatctcg gtcaggcacg
     8281 cgcctatatg gcgaagaccc tgctgggcgc ggcggcgcga aaagcaaaac tggaaatcat
     8341 cgacaatccg aacgacgcag aaatggcgat tgttctcggt gattccatcc cgaatgacag
     8401 cgcgctgaac ggtaaaaatg tctggctggg tgatatttcc cgggcagttg cgcaccctga
     8461 gctgttcctg agtgaagcca aaggccatgc gaaaccttac actgcgccgg tcgctgcgac
     8521 agcaccggtt gctgccagcg gtccgaaacg cgtagttgcg gtgactgctt gcccgactgg
     8581 cgtagcacac acctttatgg cggctgaagc cattgaaacc gaagcgaaaa aacgtggctg
     8641 gtgggtgaaa gttgaaaccc gtggttctgt tggcgcgggc aatgcaatca cccctgaaga
     8701 agtcgcagca gcggatctgg tgattgtggc ggcagatatc gaagtggatc tggcgaaatt
     8761 tgctggtaaa ccgatgtatc gcacctctac cggtctggcg ctgaagaaaa ctgcgcagga
     8821 actggataaa gcggttgctg aagcaacgcc gtatgaacca gcgggcaaag ctcaaacagc
     8881 gaccactgaa ggtaagaaag agagtgcagg cgcttaccgt cacttgctga cgggcgtctc
     8941 ttacatgctg ccgatggttg ttgcaggtgg tctgtgtatc gcgctttctt ttgcttttgg
     9001 tatcgaagcg tttaaagagc cgggtacgtt ggcagcggcg ctgatgcaga ttggcggtgg
     9061 ttcagccttt gcgctgatgg tgccggtact ggcaggttat attgccttct ccattgccga
     9121 tcgtccgggc ctcacgccgg ggctgattgg cggtatgctg gctgtcagca ccggttctgg
     9181 cttcattggc ggtattattg cgggcttcct ggctggttac attgcgaagt taatcagtac
     9241 gcaactgaaa ctgccacaga gtatggaggc gctgaaacca atcctgatca ttccgctaat
     9301 ttccagtctg gtggtcggtc tggcgatgat ctacctgatc ggtaaaccgg ttgctggcat
     9361 tctcgaaggg ctgactcact ggctgcagac catggggact gcgaatgcgg ttctgctggg
     9421 ggcgatcctc ggtggcatga tgtgtactga catgggcggt ccggtaaata aagcagcgta
     9481 cgcgttcggt gtgggtctgc tgagtactca aacctatggc ccgatggcgg cgattatggc
     9541 ggcaggtatg gtgccaccgc tggcaatggg tctggcaaca atggtggcgc gtcgcaaatt
     9601 cgacaaagcg cagcaggaag gtggcaaagc cgctctggta ttgggactgt gcttcatttc
     9661 ggaaggtgca attccgtttg ctgctcgtga tccgatgcgt gtgctgccgt gctgtatcgt
     9721 gggtggggcg ctgactggcg caatctcaat ggcgattggt gcgaaactga tggcaccgca
     9781 cggtggtctg tttgttctgc tgatccctgg cgctattacg ccggtactgg gttacctggt
     9841 agcaattatt gccggtacgc tggtggcggg tttggcctat gccttcctga aacgtccgga
     9901 agtggacgca gtagcgaaag cagcgtaata aaaggtgtgt tacagggcag aaatttcctg
     9961 ccctgtatca ttggatgagc gcaaagggaa tgaccggttt gtaaatgtaa ttttatgaat
    10021 ttaaatggtt ttatctttca gtagaaacct gaaacgttca tcaaacacta aggttatttc
    10081 ctcaagggca gattattttc tgcccttttt tagtgttcct gttctgtgca tcccgtcaca
    10141 aattcctcgc ttttgtaatt caggcattta atcgcatttt gtgagcctgc tcaaatctgc
    10201 ccattttgcc tctctggcgg taatttactt tacgtaaaac atgcatacta tgagcacatg
    10261 tttaaatatg aacacatgtt taaaccgtct ttgcagtatc tatattcttg tggattgaca
    10321 gggttggtaa ttttttacca gaggacgtgc tatgcgcgaa aaggattatg tcgtaattat
    10381 aggttcggcg aatattgatg tcgccggata ttcacatgaa tcattaaatt atgcggattc
    10441 aaatccaggt aaaataaaat ttacgcctgg tggagttggg cgcaatattg cacaaaacct
    10501 ggcgttgctg ggtaacaaag cctggctact gagcgcagta ggcagtgatt tttatggtca
    10561 atcgctgcta acgcaaacca atcaatctgg cgtttatgtc gataaatgcc tgattgtgcc
    10621 gggagaaaat acgtctagtt atttatcatt actcgataat accggtgaaa tgctggttgc
    10681 tataaatgac atgaatatta gcaacgctat tacagctgaa tatctcgcac agcaccgtga
    10741 atttattcag agggcaaagg tcattgtcgc ggactgtaat atcagtgaag aggcactggc
    10801 atggattctg gataatgccg ccaacgtacc cgtatttgtc gatccggttt cagcgtggaa
    10861 atgtgtcaaa gtgcgcgacc gtctaaatca gatccacact ctgaagccaa accgccttga
    10921 agcggaaacc ctgagtggga ttgcgctgtc agggcgtgaa gatgtggcaa aagttgctgc
    10981 ctggttccat caacatggcc tgaaccgact ggtattgagc atgggcggcg atggcgttta
    11041 ttacagtgat atcagcggtg aaagtggctg gtctgccccg atcaaaacca atgttattaa
    11101 tgttaccgga gcgggcgatg ccatgatggc gggacttgct tcgtgttggg tagacggaat
    11161 gccgtttgcc gaatctgttc gtttcgcaca gggatgttcg tcaatggcgc tctcctgtga
    11221 atacaccaat aaccccgatt tatcgattgc caacgttata tcgttagtgg agaacgcaga
    11281 atgtctgaat taaaaatttc ccctgaatta ttacaaattt ccccggaagt gcaggacgct
    11341 ttaaaaaaca aaaaaccggt tgtggcgctg gaatcgacca ttatttctca cgggatgccg
    11401 ttcccacaaa atgcccggac cgcaattgaa gtagaagaaa ctattcgtaa acagggcgca
    11461 gtacctgcca ctatcgcaat tattggcggc gtgatgaaag tgggtttaag caaagaagaa
    11521 attgaattac tgggtcgtga agggcataac gtgaccaaag ttagtcgtcg cgatttacct
    11581 tttgttgttg ccgccggaaa aaatggcgca accactgtgg cttcaacgat gattattgcg
    11641 gcgcttgccg gaattaaagt atttgccacc gggggaattg gtggtgtgca tcgcggggcg
    11701 gaacatacct tcgatatttc tgccgatttg caagaactgg caaatactaa tgtcaccgtt
    11761 gtttgtgccg gggcgaaatc tattctcgat ttaggattaa ccactgagta tttagaaacc
    11821 ttcggtgtgc cgttaattgg ctatcagact aaagcgctgc ctgcgttttt ctgccgtacc
    11881 agcccgtttg acgtcagcat tcgtctcgac agcgccagtg aaattgcccg tgcaatggcg
    11941 gtgaagtggc aaagcgggct gaacggtggc ctcgtggtag cgaacccgat cccggaacag
    12001 tttgcgatgc cggaacacac tatcaatgcg gcgatcgatc aggcggtagc tgaagctgaa
    12061 gcgcagggtg ttattggtaa agaaagtacg ccattcctgc tggcgcgcgt tgctgaactg
    12121 accggcggtg acagcctgaa atccaacatc cagctggtgt tcaacaacgc cattctggcg
    12181 agtgaaattg ccaaagaata ccagcgtctc gcgggttaat ttctgacggg cagaaagccg
    12241 tcgcccgtac tcatcaaaca taccaaaacc tggcgctgac gccgggttta ctggcatgaa
    12301 gggaaaatca ttatggacat aatgagaagt gttgtgggga tggtggtgtt actggcaata
    12361 gcatttctgt tgtcagtgaa taaaaagagc atcagtttgc gtacggttgg agccgcacta
    12421 ctgctgcaaa ttgctattgg tggcatcatg ctctattttc cgccgggaaa gtgggctgta
    12481 gaacaggcgg cattaggcgt tcataaagtg atgtcttaca gtgatgccgg tagcgccttc
    12541 atttttggtt cgctggtcgg gccgaaaatg gatgtcctct ttgacggtgc gggttttatc
    12601 ttcgcctttc gcgtactccc ggcgattatc ttcgtcactg cgctcattag tctgctgtac
    12661 tacattggcg tgatggggct gctgattcgc atccttggta gcattttcca gaaagccctc
    12721 aacatcagca aaatcgaatc ttttgttgcg gttaccacta ttttcctcgg gcaaaatgag
    12781 atcccggcga tcgttaaacc gtttatcgat cgcatgaatc gcaacgagtt gtttaccgca
    12841 atttgtagcg ggatggcgtc cattgcgggt tcgatgatga ttggttatgc cggaatgggc
    12901 gtaccaattg actacctgtt ggcggcatcg ctgatggcga tccccggcgg tattttgttt
    12961 gcacgtattc ttagtccggc aaccgagcct tcgcaggtca catttgaaaa tctgtcgttc
    13021 agtgaaacgc cgccaaaaag ctttatcgaa gcggcggcga gcggtgcgat gaccgggttg
    13081 aaaatcgccg ccggtgtggc gacggtggtc atggcgtttg tcgcaattat tgcgctgatt
    13141 aatggcatta tcggcggaat tggcggctgg tttagtttta ccaatgcttc tctggaaagt
    13201 atttttggct atgtgctggc accgctggcg tggatcatgg gtgtggactg gagtgatgcc
    13261 aatcttgcgg gtagcctgat tgggcagaaa ctggcgatta acgaattcgt cgcttacctg
    13321 agtttctccc catacctgca aacgggcggc acgctggaag tgaaaaccat tgcgattatc
    13381 tcctttgcgc tttgtggttt tgctaacttt ggttctatcg gtgttgtcgt tggcgcattt
    13441 tcggctattt cgccaaaacg tgcaccggaa atcgctcagc ttggtttacg ggcgctggca
    13501 gcagcaacgc tttccaacct gatgagcgcg accattgccg ggttctttat tggtttagct
    13561 tgagtttcat tgccggatgt actcgcttat taagcgttgc atccggcaat cgcgctattg
    13621 caaataattt ttactgcatc atcccggaga atttattctc cgggtccatc tccagcgcca
    13681 gccctgacaa ctgctttctg tttttaatga gatatccttt cttgctttta attaataaac
    13741 cgtcgtgaat gaattgcgcg agaacatata aaagatgtcg ataagaaacg cctaaatact
    13801 ctgccgcttg cgtatgcttt tcgtgataaa gatcaccttc ctgcgagagt aatataaaag
    13861 ctgccaggcg gttcactaac ggaaatgatt gattctgagt taaagaaaca atattgcgat
    13921 aatttttatg gcttaaagaa acgcagagct ttcgtaaaaa tagggtatcg tttaataaca
    13981 gcggacggta atgtttcata gggagcgcaa ggcaccaaca ctcttcaatc gcctgtactg
    14041 cacgtggttc atggtcttta tcgattaact caatctcgcc aataaaacag ggggcggcaa
    14101 agaaatcgat cagcgacacg cgaccattag ctagcgtggc gtaaagcctg gcgcggcctc
    14161 gcgtcaggta aaacagccag gacggctgtt gcccttcctg cacaatgtag tcacgcgcta
    14221 aaaaatgaaa cagccgcgta tcggctgaaa catccgttaa aaagcaatcc ttaaacgcgg
    14281 attcactcat caattgcagc ttaagatcat tattgtggat ttctttcacg atgtttatcc
    14341 gctgtgagat ttctcatatt tagcatacgt acgctgttgt taagattcaa tcatcggccc
    14401 ggtcgaattt tctttgctgt agaaaaaata acgcaactgg aaacagagga aataaacaat
    14461 ggaaaagaga aaaattattc tggattgtga tccgggtcat gatgatgcta ttgcaatgat
    14521 gatggcagcg aaacatccgg caatagattt attaggcatc actattgtag cgggtaatca
    14581 gacgcttgat aaaacattaa ttaatggact gaatgtttgc cagaaactgg agattaatgt
    14641 tccggtttat gcggggatgc cgcaacccat tatgcgtcaa caaatcgttg ccgataatat
    14701 tcacggtgaa accggactgg atggcccggt attcgagccg ctgacccgcc aggcagaaag
    14761 tacccacgcg gtgaaataca tcattgatac cctgatggca agcgatggcg atatcactct
    14821 ggtgccggtt ggtccgcttt caaatatcgc ggtggcaatg cgtatgcaac ccgcgatcct
    14881 gcccaaaatc cgtgaaattg tgctaatggg cggcgcttac ggtacaggca acttcacgcc
    14941 atctgccgag ttcaacatct ttgctgaccc ggaagccgca cgcgtggtgt tcacctccgg
    15001 cgttccatta gtgatgatgg gcctcgatct taccaaccag accgtttgca ccccggacgt
    15061 aattgctcgg atggaaaggg caggcggccc tgccggagag ctgttcagcg acataatgaa
    15121 cttcactctc aaaacccagt tcgaaaacta cggcctggct ggcggcccgg tgcacgacgc
    15181 cacctgcatc ggttatctga ttaaccctga tggcattaaa acccaggaga tgtacgtcga
    15241 agtggacgtc aacagtggcc cttgttatgg gcgtaccgtc tgcgacgagc tgggcgttct
    15301 tggcaagccc gctaatacca aagtcggcat cactattgat accgactggt tctggggctt
    15361 agtcgaagag tgcgtgcgcg gctacatcaa aacccattaa gtgttgataa caagccgggc
    15421 cggtatcgca agatgcaggc ctggctattt ctgatgggca gaaagccgtc gcccaatatg
    15481 acatgaagag ataatcacta tggatgtcat gagaagtgtt ctgggaatgg tggtattgct
    15541 gacgattgcg tttttactgt cagtaaacaa gaagaagatc agcctgcgta ccgttggcgc
    15601 ggcgttagtg ttacaggtcg tgattggcgg cattatgctt tggttaccgc cagggcgttg
    15661 ggtcgctgaa aaagtcgctt ttggcgtgca taaagtgatg gcgtacagcg acgcgggtag
    15721 cgcatttatc ttcggttctc tggtcggacc gaaaatggac accttatttg acggcgcagg
    15781 atttatcttt ggtttcaggg tattaccggc aattatcttc gtcactgcac tggtgagtat
    15841 tctctactac atcggtgtga tggggatctt aattcgcatt ctcggcggta tcttccagaa
    15901 agcgttaaac atcagcaaga tcgagtcatt cgtcgcggtc actaccattt tcctcgggca
    15961 aaacgaaatt ccggcgattg tgaagccctt tatcgatcgg ctgaatcgca atgaattatt
    16021 tacagcgatt tgtagcggca tggcctcgat tgctggttcg acaatgattg gttatgccgc
    16081 actgggcgta cctgtggaat atttgctggc ggcatcgtta atggcgatcc caggcggaat
    16141 cttgtttgcc cgcctgttaa gcccggctac ggaatcttcg caggtttctt ttaataacct
    16201 ctctttcacc gaaacaccgc caaaaagcat tattgaagcc gccgcgacag gggcaatgac
    16261 cgggctgaaa atcgccgcag gtgtggcgac agtagtgatg gcatttgtcg ccatcattgc
    16321 gttaattaac ggtattatcg gcggcgttgg cggctggttt ggttttgaac atgcctcgct
    16381 ggagtccatt ttaggttacc tgctggcccc actggcgtgg gtgatgggtg ttgactggag
    16441 tgatgcgaat cttgccggga gtttgattgg acagaaactg gcaataaatg aatttgtcgc
    16501 ttacctcaat ttttcaccct atctgcaaac ggctgacacg ctggatgcta aaaccgtggc
    16561 gattatttcc ttcgcgttgt gcggtttcgc taactttggt tctatcgggg tggtggtggg
    16621 ggcgttttct gcggttgcgc cacaccgtgc gccggaaatc gcccaacttg gtttacgcgc
    16681 gctggcggcg gcgacacttt ccaatctgat gagtgccacc attgccgggt tctttattgg
    16741 tttagcgtag tgtgcgattt atgagccgga ttagatgggg tcaagcgtcg aatccggcaa
    16801 cattgttaca tcattggcac taatgacagc gcgttatcgg cagacagggt agggttgttt
    16861 aagctgccgc tggcgcgtga aattgccgcg caggccatcg caaaacgggc gctgtcacgg
    16921 aaattgtttc cttccagaaa gctatatacc aggcccgcca taaaaccatc gtcagcacca
    16981 aaactgtcta ctgtcgtatg cgctggtgcg gtcagcaaaa attgttctcc atccttttcg
    17041 ctgcaataaa ctgactcatc gggcaaataa acaaacagtt gctgaacacc ttgctgatgc
    17101 aacgcattca ctgcagcatt acggtcagca tcgctggtga tcgcctgtcc ccataaaatt
    17161 tccagctccg gtaaagtggg tttcagggtg tgaatatgcg ccagccagtg tttgattttg
    17221 cccgctttga attctgaaac ggtatcgaca aacaccggga tctcaccagc gagggtaaag
    17281 acccattcca gcgcctcggc tgtcaggttg caatcagcca gtaccacgcc cgcatgacga
    17341 agtaaatcgc gcgacccgtt cagtagttgc ggtgtcaact gttccagcag atgggtatcg
    17401 ttaatcgcca gcacggtttg atcgtctcga ttggcaattg ccagatacgt cgatgtgctt
    17461 tgaccatgca aacgaacgca gccggagaca ttcacgccgg cacggcgcgt ttcttccagg
    17521 agcatttcgc cataaaagtc atcgccaatc actgaaagca aatggacgtc gcggcctaac
    17581 agcgccagat tgtgggcgat attgcgtcca acgctgcctg ccgagcaatg aattgtaccg
    17641 ggatgagaag ccgcttgcgg gtaatggata tccgccatcc cgcgaatatc catattgatt
    17701 gtccccacca ctacgcagta ttcctgctcg gtgagaatgt aacctttgcc tttaatccgc
    17761 cctttgcgca ttaaatccat aatatgcgcc gcaacgcgcg aacgactgat ttgcagcatg
    17821 tccgcaattt cgttctgctg aatcagcggg ttacgccgta aaattgcaag gatctccttt
    17881 tcccggttat tcatcttcag gctaccgctt tttcagtttg ttgcgctttc agccaggcga
    17941 tctcttctgc ccagatatcc gggttgatgg tttcaaggat cagcggaatg ccgtcgaaac
    18001 ggtcgtcctg catgatccag cggaacgcat catgaccgat attgccttca ccgaggctat
    18061 gatggcggtc aacgcggctg ccaaaggtgc ttttcgcatc gttaaggtgc atcccgcgca
    18121 gatacttaaa gccgacaata cgggcaaaat cagcgaacgt tttctcgcat tcggctggag
    18181 tacgcaaatc atacccggca gcgaaagcat ggcaggtatc aatgcagacg ccgacacggg
    18241 atttatcttc cacgccgtcg ataatcgccg cgagatgttc gaatttaaac cctaagttac
    18301 tgccctgacc ggcggtgttt tcaatcaccg cggtcacacc ttgagtttta tccagcgcaa
    18361 tgttgatgga ttcggcaata cgcgcaaggc aatcctcttc tgaaatctgc atcagatggc
    18421 tgccagggtg gaagttgagc aaagaaagcc ctagttgttc gcaacgctgc atttcatcta
    18481 taaaggcatc gcgcgatttt tccagagctt cagtgaccgg atgtccgagg ttaatcagat
    18541 aactgtcgtg gggaagaatt tgcgccgatg tgtagtgata tttttcacag gcggctttga
    18601 atttatcgat ggtttgcgtc gtgagcggtg cggcacgcca ctgacgttgg tttttggtga
    18661 acaaggcaaa cgcggttgcg tcgatttcgg cggcgcgaat tgcggcattt gccagaccgc
    18721 cagcagcact aacgtgcgct ccaatgtatt tcatgcgagg actcctgtta aacccgctgg
    18781 aggaaaacgg taatgatagc gggttaacag gagcaagatg tagtggttta tgcgatgacg
    18841 ctttgaatca catagttaat cgcaccacca ccaacaatca gccaggcaaa cagtaccagt
    18901 gccatcagca gaggtttcgc cccggctttt ttcagcgcgc tgacgtgagt ggtcagaccc
    18961 agcgccgcca tcgccattgc cagcaggaag gtatccagcg ttaccagcat gttcaccacg
    19021 ctctgcggta acaggtggaa cgagttaaag atggcaacta cgatgaacaa gatggcaaac
    19081 cacggaatag tgattttgct tttctcgcca ctgttcgccc cagacagctg tttaacacgc
    19141 gccgccagca ggatgaggaa cggagccagc atcatcacgc gcagcatttt ggaaataact
    19201 gctgcgtttt ccgcatccgg gctgatggca tgacctgctg ccaccacctg cgccacttcg
    19261 tgcacagtag aaccaatgta gataccgaaa gtttccggac taaaccattg agacatcagc
    19321 ggatatatcg ccgggtagag gaaaatcgcg acggtcccga agataacaac ggttgcaaca
    19381 gccacggtta ctttactggc ttccgctttc actactggct cagtcgccag taccgcggca
    19441 gcaccacaga tactgctacc ggcaccgatc aaccagctgg tgtgcttatc cagaccaaac
    19501 actttctgcc ccaggaagca agccagcagg aaggtactgg acagcgtcag cacgtcaatg
    19561 atgatcccac tgataccaac atcggcaatt tgcgagaacg tcagacggaa gccataaaga
    19621 atgataccca gacgtaataa atattgcttg gcaaacagca caccaccgtc acagcttttc
    19681 cagatgtgcg gatagatggt gttgcctaaa accatcccca acaagattgc gagggtgagg
    19741 gcactaaacc cggcacccgc aaccgcggga atggaaccac cccacagggc gaccccggtg
    19801 ataactgcac tcagggctaa ccccggaata aaatgccaca gtgtacgatg ttgtttctgt
    19861 aaggtgatat tcgtcataac cctctccttt aaccgggcat aaggttacga ctaactggtt
    19921 taaaaataaa attgattata tatttataat taatctttat aagtggtaag caactatgca
    19981 catcaccctc cggcagttgg aagtttttgc agaagtattg aaaagtggat caaccaccca
    20041 ggcgtcggtg atgctggcgt tgtcgcaatc agcagtgagc gcagccttga ccgacctgga
    20101 agggcagctt ggcgtgcaac tgtttgatcg cgtggggaaa agactggttg ttaatgaaca
    20161 cgggcggctg ctctatccgc gtgcgttggc attgcttgaa caggcggttg aaatcgaaca
    20221 actgtttcgc gaagacaacg gcgcgattcg tatctatgcc agtagtacca tcggtaacta
    20281 cattctgcct gcagttatcg cccgttatcg ccatgattat ccgcagttgc cgattgaact
    20341 tagcgttggg aacagccagg acgtgatgca agcggtgctg gatttccgcg ttgatattgg
    20401 ctttattgaa ggaccgtgcc acagcactga aatcatttct gaaccgtggc tggaagacga
    20461 gctggtggtt ttcgccgcgc cgacttcgcc gttggcccgt ggtccggtca ccttagaaca
    20521 gctggccgct gcgccgtgga tcctgcgtga acgcggttcc ggcacgcggg agattgtcga
    20581 ttatctgttg ctgtcacatt taccgaagtt tgagatggcg atggaattag gtaactccga
    20641 ggcaatcaaa catgcggtgc gtcatgggtt gggaattagt tgcctgtcgc gacgtgtgat
    20701 tgaagatcaa ttgcaggcag gcacattaag tgaagttgcg gtccctctgc cgcgcctgat
    20761 gcgtacgttg tggcgtatac atcatcggca aaaacacctt tccaacgcgc tacggcgctt
    20821 tctggactat tgcgatcccg caaatgtgcc gcgttaagtt gctgtacaag aacatgctgg
    20881 tgctgtgtcg atttcgtgac gcagcgcctt cagcatgcat tcgccagaaa agagattggc
    20941 tgctttactt ataatccctg ggcgatcatg aaggtgtctt ataaccgtgt atttctgccg
    21001 gaaggattgc caatcgtctg ctacaatcgc gcctcatttt ttagatggat agcatttttg
    21061 tatggtttcc gaaactaaaa ccacagaagc gccgggctta cgccgtgaat taaaggcgcg
    21121 tcacctgacg atgattgcca ttggtggttc catcggtaca ggtctttttg ttgcctctgg
    21181 cgcaacgatt tctcaggcag gtccgggcgg ggcattgctc tcgtatatgt tgattggcct
    21241 gatggtttac ttcctgatga ccagtctcgg tgaactggct gcatatatgc cggtttccgg
    21301 ttcgtttgcc acttatggtc agaactatgt tgaagaaggc tttggcttcg cgctgggctg
    21361 gaactattgg tacaactggg cggtgactat cgccgttgac ctggttgcag ctcagctggt
    21421 catgagctgg tggttcccgg atacaccggg ctggatctgg agtgcgctgt tcctcggcgt
    21481 tatcttcctg ctgaactaca tctcagttcg cggctttggt gaagcggaat actggttctc
    21541 actgatcaaa gtcacgacag ttattgtctt tatcatcgtt ggcgtgctga tgattatcgg
    21601 tatcttcaaa ggcgcgcagc ctgcgggctg gagcaactgg acaatcgggg aagcgccgtt
    21661 tgctggtggt tttgcggcga tgatcggcgt agctatgatt gtcggcttct ctttccaggg
    21721 aaccgagctg atcggtattg ctgcaggcga gtccgaagat ccggggaaaa acattccacg
    21781 cgcggtacgt caggtgttct ggcgaatcct gttgttctat gtgttcgcga ttctgattat
    21841 cagcctgatt attccgtaca ccgatccaag cctgctacgt aacgatgtta aagacatcag
    21901 cgttagtccg ttcaccctgg tgttccagca cgcgggtctg ctctctgcgg cggcggtgat
    21961 gaacgcagtt attctgacgg cggtgctgtc agcgggtaac tccggtatgt atgcgtctac
    22021 tcgtatgctg tacaccctgg cgtgtgacgg taaagcgccg cgcattttcg ctaaactgtc
    22081 gcgtggtggc gtgccgcgta atgcgctgta tgcgacgacg gtgattgccg gtctgtgctt
    22141 cctgacctcc atgtttggca accagacggt atacctgtgg ctgctgaaca cctccgggat
    22201 gacgggtttt atcgcctggc tggggattgc cattagccac tatcgcttcc gtcgcggtta
    22261 cgtattgcag ggacacgaca ttaacgatct gccgtaccgt tcaggtttct tcccactggg
    22321 tccgatcttc gcattcattc tgtgtctgat tatcactttg ggccagaact acgaagcatt
    22381 cctgaaagat accattgact ggggcggcgt agcggcaacg tatattggta tcccgctgtt
    22441 cctgattatt tggttcggct acaagctgat taaaggaacc cacttcgtac gctacagcga
    22501 aatgaagttc ccgcagaacg ataagaaata agtttctccc ttccttgcta agccctctca
    22561 accgagaggg ctttttcaat tccatttccc tgacaaatca tgcggatata aaatttaaca
    22621 tttggattga taattgttat cgtttgcatt atcgttacgc cgcaatcaaa aaaggctgac
    22681 aaatcagagg ctggtccggc tttctgggat gatcacctgc atacaaataa gtccaccgcg
    22741 atgctgccgt acgcaagggg acgtgaagaa gatgtgagcg ataacccatt ttattttcgt
    22801 agttacctca tggagatatg gaatgtttag gttgaaccct ttcgtacggg tcgggctgtg
    22861 tttgtccgct atttcttgtg catggcctgt gttagcggtc gatgacgatg gcgaaacgat
    22921 ggttgtcact gcatcttccg ttgaacaaaa ccttaaagat gcacccgcca gtatcagcgt
    22981 cattacccag gaagacctgc agcgaaaacc ggtacagaat ctgaaagatg tcctcaaaga
    23041 agtgcctggc gtacaactga cgaacgaagg ggataaccgt aagggcgtta gtattcgtgg
    23101 tctggacagc agctacaccc tgattcttgt cgacggtaaa cgcgttaact cccgcaatgc
    23161 cgtcttccgc cacaatgatt tcgatctgaa ctggatcccg gtcgattcca tcgaacgtat
    23221 tgaagtggtc cgtggcccga tgtcgtcgct gtacggttcc gatgcgctcg gcggtgtagt
    23281 gaatatcatc accaaaaaaa tcggtcagaa atggtcgggt accgttaccg tcgataccac
    23341 cattcaggaa catcgcgatc gcggtgatac ctataacggt cagttcttta ccagtggacc
    23401 attaattgat ggtgtgctgg gaatgaaagc ttacggcagc ctggcaaaac gtgaaaagga
    23461 tgacccgcaa aactcaacga ccaccgatac cggagaaacg ccgcgtattg aaggattctc
    23521 cagccgcgac ggcaatgtcg aatttgcctg gacaccgaat caaaatcacg attttactgc
    23581 cggatacggt ttcgaccgtc aggatcgtga ttccgactcg ctggacaaaa accgcctgga
    23641 acgccagaac tactccgtca gccataatgg gcgttgggat tacggcacca gcgaactgaa
    23701 atactacggt gagaaagtcg agaacaaaaa ccctggcaac agcagcccga taacttccga
    23761 aagcaatacg gtcgacggca aatacacgtt gccgctgacg gcgattaatc agtttctcac
    23821 ggttggcggt gaatggcgtc acgacaaact tagcgatgcg gtgaacctga ccgggggaac
    23881 cagctccaaa acgtctgcca gccagtacgc gctgtttgtg gaagatgaat ggcggatctt
    23941 cgagccgctg gcgctgacga ccggcgtgcg tatggacgat cacgaaacct acggtgaaca
    24001 ctggagtccg cgtgcctacc tggtttataa cgccaccgac accgtaacgg tgaaaggggg
    24061 ctgggcgacg gcatttaaag caccttctct gttgcaactt agccctgact ggacgagcaa
    24121 ttcctgccgt ggcgcatgta agattgtggg tagcccggat ctgaaaccag aaaccagcga
    24181 aagttgggag ctggggcttt actacatggg tgaagaaggc tggctggaag gggttgaatc
    24241 cagcgttacc gttttccgta acgatgtgaa agatcgtatc agcatcagcc gtacgtctga
    24301 cgtcaatgct gcaccgggct accaaaactt tgttggtttt acagcgaacg gtgtgccggt
    24361 atttagctac tacaacgtta acaaagcacg tattcagggc gtggaaaccg aactgaaaat
    24421 tccgttcaac gatgaatgga aactgtcgat caactacacc tacaacgatg gtcgtgatgt
    24481 cagcaacggc gaaaacaaac cgctatccga tctgccgttc catactgcta acggtacgct
    24541 ggactggaaa ccgctggcgc tggaagactg gtcattctat gtttctggtc actataccgg
    24601 gcagaaacgc gccgacagcg cgacggctaa aacaccgggc ggttatacca tctggaatac
    24661 cggcgcggcc tggcaggtga ctaaagacgt caaactgcgc gcaggcgtgc tgaaccttgg
    24721 cgacaaggat ctcagtcgtg acgactacag ctataacgaa gacggacgtc gttactttat
    24781 ggcagtggat tatcgcttct gatgagaaga tgcccggcga accgggcgga ctttcacttc
    24841 agtaaatact gcgcatggaa gcgcaggtga tcctctataa aagaggcgat gaagtagtaa
    24901 ctgtgatcat atcccggctg aatacgcagc gtcatcggcc atgctttctg gcgcgcggct
    24961 tctgcaagta ctgcaggttg cagttggtca gcaagaaact gatcgttatc accctgatcg
    25021 ataagcgtcg ggatcgcatc ctgcgcgtta ctggcataca tcagcgcgca actgtcccat
    25081 tccagccatg catttttgtc ctcacctaaa tagcggctaa acgctttgat tccccacggg
    25141 acgctgcacg gattcacaat tggcgcaaag gccgaaacgc tggtgtattt gcctgggttt
    25201 ttcagcgcca taatcagcgc accgtgacca cccattgaat gaccgctaat agcgcagcgg
    25261 tcgctgacat taaattgcga ctgaaccagc gccggtaatt catcgcgcag ataatcatac
    25321 atccggtaat gcgtcgccca cggcggttgc gtggcattaa gataaaagcc tgcgccctgg
    25381 cctaaatcgt agccatcgtc gttggcaacc ttttcgccgc gcgggctggt gtctggcata
    25441 accagtacaa tccccagttc cgccgccacg cgctgggcac ccgccttggt ggtgaagttc
    25501 tcgtcattgc aggttaatcc ggaaagccag tacagcactg gtggcggggt gtgatcacga
    25561 gggggtggga gaaagatact gaacgtcatc gggcagttta aggtactgga gtcgtgtcgc
    25621 cagcgttgct gccagccttc aaaacagcgg tgctcttcga gcatttccat gcgtggctcc
    25681 ttgttgtgtt gtttgcaatt tcttcatcat accgtttatt caatgtgctg tgagtaactt
    25741 tcacttccgt atttgcataa cgatgtttta acatctgctg atgaaaggca gcggcaatta
    25801 caataattat cgctgtgaat actggattat gtgcgccgcc tcacgcacaa taatcaggct
    25861 gtaaatcagc ttaataactt tgcccccacg cagggcggag gcgtcacacc tgcaggagaa
    25921 atcataaatg ccatcactca gtaaagaagc ggccctggtt catgaagcgt tagttgcgcg
    25981 aggactggaa acaccgctgc gcccgcccgt gcatgaaatg gataacgaaa cgcgcaaaag
    26041 ccttattgct ggtcatatga ccgaaatcat gcagctgctg aatctcgacc tggctgatga
    26101 cagtttgatg gaaacgccgc atcgcatcgc taaaatgtat gtcgatgaaa ttttctccgg
    26161 tctggattac gccaacttcc cgaaaatcac cctcattgaa aacaaaatga aggtcgatga
    26221 aatggtcacc gtgcgcgata tcactctgac cagtacctgt gaacaccatt ttgttaccat
    26281 cgatggcaaa gcgacggtgg cttatatccc gaaagattcg gtgatcggtc tgtcaaaaat
    26341 taaccgtatc gtgcagttct ttgcccagcg tccgcaggtg caggaacgtc tgacgcagca
    26401 aattctcatt gcgctacaaa cgctactggg caccaataac gtggctgtct ctatcgacgc
    26461 ggtgcattat tgcgtgaagg cccgtggcat ccgcgatgca accagtgcca cgacaacgac
    26521 ctctcttggt ggattgttca aatccagtca gaatacgcgc cacgagtttc tgcgcgctgt
    26581 gcgtcatcac aactgattaa aaggcaggaa ccatggagcg caacgtcacg ctcgattttg
    26641 ttcgcggcgt cgccattctg gggatcctgc tattaaacat cagcgccttt gggctaccaa
    26701 aggcggctta tctcaatccc gcctggtacg gtgctattac gccgcaggat gcatggacct
    26761 gggcatttct tgatctcatc ggccaggtga aattcctcac gctttttgcg ctgctgtttg
    26821 gtgctggact gcaaatgttg ctgccccgtg gcagacgctg gatccagtcg cggttaacgc
    26881 tgttagtctt attgggcttt attcacggtt tattgttctg ggacggcgat attctgctgg
    26941 cttacgggct ggtgggctta atctgctggc ggctggtgcg cgatgcgcca tcggtaaaaa
    27001 gcctgtttaa taccggcgtc atgctttatc tggtggggct tggcgttttg ctgttattgg
    27061 ggctgatttc cgacagccag accagccgcg cctggacgcc ggatgcatcg gctattttgt
    27121 atgaaaaata ctggaagctt cacggcggcg ttgatgcgat cagtaatcgt gccgatggtg
    27181 ttggcaacag tttactggca ctgggcgcac agtatggctg gcaactggct gggatgatgc
    27241 tcattggtgc ggcgctgatg cgcagcggct ggctgaaagg gcagttcagc ttacgtcact
    27301 atcgtcgtac tggttttgtg ctggtggcga ttggggtgac cattaacctt cctgccatcg
    27361 ccctgcaatg gcagctggac tgggcgtatc gctggtgtgc cttcttactt caaatgccgc
    27421 gggaactgag tgcgccgttt caggcgattg gctatgcgtc gctgttttat ggtttctggc
    27481 cgcaattgag ccgctttaag ctggtgctgg cgatcgcctg cgtcggacgg atggcgctga
    27541 ccaactatct attgcaaacg ctgatttgta ccacgctttt ttaccacctc ggtctgttta
    27601 tgcagtttga ccgtctggaa ttgctggcgt ttgttattcc ggtatggctg gcgaatattc
    27661 tcttctctgt tatctggctg cgttacttcc gccaggggcc ggtggaatgg ctctggcgtc
    27721 agttaacttt gcgtgctgcc ggaccggcaa tatctaaaac atcaagataa cgatctggat
    27781 cacattcgtt aacaaaatgg ctgtaaccgt ttccattgct gtgactcgat tcacgaagtc
    27841 ctgtattcag tgctgacaaa atagccgcca gcaagcagtc atttactgca atctcataac
    27901 aggtagtgaa tatgatcacc attcgtgatg tagcgcgtca ggctggcgtc tctgtggcaa
    27961 cggtttcccg ggtgctcaat aacagcacgc tggtcagtgc cgacacgcgt gaagcagtaa
    28021 tgaaagccgt gagtgagctg gattatcggc caaacgccaa tgcccaggcg ctggcaactc
    28081 aggttagcga caccattggc gtggtggtga tggacgtttc tgatgcgttt ttcggcgcgc
    28141 tggtaaaagc ggtggatcta gtcgctcagc agcatcagaa atacgtgcta atcggcaata
    28201 gctatcatga agcggaaaaa gagcgtcacg ccattgaggt gttaattcgc cagcgttgta
    28261 atgcgttgat tgttcactca aaagcattga gtgacgatga actggcgcaa tttatggata
    28321 acattcccgg tatggtgtta atcaaccgcg ttgtgccggg gtacgcccat cgttgcgttt
    28381 gcctggataa tctcagcggt gcccgaatgg cgacgcgcat gttgctgaat aacggtcatc
    28441 aacgtattgg ttatctttct tccagtcacg gcattgaaga tgacgccatg cgtaaagcag
    28501 gctggatgag tgcgttgaaa gagcaggata ttattccgcc ggaaagctgg attggcactg
    28561 gtacgccgga catgccgggc ggtgaggcgg cgatggttga actgctgggg cgcaatctac
    28621 aacttaccgc tgtatttgct tataacgaca atatggccgc tggcgcactg acagcattaa
    28681 aagataatgg cattgcgatt ccgttacatc tctcaatcat cggtttcgat gatattccca
    28741 tcgcccgtta caccgacccg caattaacga ccgtgcgtta tcccattgct tcaatggcta
    28801 aattagccac cgaactggcc ttgcaggggg cagcaggcaa tattgatcct cgtgccagcc
    28861 actgttttat gccgacgtta gtgcgtcgcc attctgtcgc aacgcgccag aatgcggcgg
    28921 cgatcactaa ctcaacaaat caggcgatgt aaccgctttc aatctgtgag tgatttcaca
    28981 gtatcttaac aatgtgatag ctatgattgc accgttttaa cgttgtaacc cgtatgtaac
    29041 agtgaataat cactttcacc gaggtaatag cgtcataaca acaattaaag ccgttttctg
    29101 gagcgttacc gggcatggaa gaacgaattt taaaaagtga gcttcggcgt tcagtaacac
    29161 ttcattaact ctactgcccc gccgagcatt tatctcaagc actaccctgc ataagaaaaa
    29221 ccggagatac catgaataag aaggtgttaa ccctatctgc tgtgatggcc agcatgttat
    29281 tcggtgccgc tgcacacgct gctgatactc gcattggtgt aacaatctat aagtacgacg
    29341 ataactttat gtctgtagtg cgcaaggcta ttgagcaaga tgcgaaagcc gcgccagatg
    29401 ttcagctgct gatgaatgat tctcagaatg accagtccaa gcagaacgat cagatcgacg
    29461 tattgctggc gaaaggggtg aaggcactgg caatcaacct ggttgacccg gcagctgcgg
    29521 gtacggtgat tgagaaagcg cgtgggcaaa acgtgccggt ggttttcttc aacaaagaac
    29581 cgtctcgtaa ggcgctggat agctacgaca aagcctacta cgttggcact gactccaaag
    29641 agtccggcat tattcagggc gatttgattg ctaaacactg gacggcgaat cagggttggg
    29701 atctgaacaa agacggtcag attcagttcg tactgctgaa aggtgaaccg ggccatccgg
    29761 atgcagaagc acgtaccact tacgtgatta aagagctgaa cgacaaaggc attaaaactg
    29821 aacagttaca gttagatacc gctatgtggg ataccgctca ggcgaaagat aagatggacg
    29881 cttggctgtc tggcccgaac gccaacaaaa tcgaagtggt tatcgccaac aacgatgcga
    29941 tggcaatggg cgcggtagaa gcactgaaag cacacaacaa gtccagcatt ccggtgtttg
    30001 gcgtcgatgc gctgccagaa gcgctggcgc tggtgaaatc cggtgcactg gcgggcaccg
    30061 tactgaacga tgctaacaac caggcgaaag cgacctttga tctggcgaaa aacctggccg
    30121 atggtaaagg tgcggctgat ggcaccaact ggaaaatcga caacaaagtg gtccgcgtac
    30181 cttatgttgg cgtagataaa gacaacctgg ctgaattcag caagaaataa gactgattgg
    30241 gcgcagtcta ttactgcgcc cttattgtat ggccaacaag gtaaaattat atggtcagct
    30301 caacgactcc gtcttccggg gaatacttgt tggaaatgag cggtatcaac aagtcttttc
    30361 ctggtgttaa ggcactggat aacgttaatt taaaagtccg gccacattct atccatgcat
    30421 taatggggga aaacggtgca ggaaaatcga cattattaaa atgcctgttt ggtatttatc
    30481 aaaaagactc cggcaccatt ttattccagg gtaaagagat cgatttccat tctgcaaaag
    30541 aagccctaga aaatggtatt tcgatggtac accaggagtt aaacctggta ttacaacgtt
    30601 cggtgatgga taacatgtgg ctggggcgat atcccaccaa aggcatgttt gtcgatcagg
    30661 acaaaatgta ccgcgaaacc aaagcgattt ttgatgaact ggatattgat atcgatccgc
    30721 gtgcgcgcgt cggcacatta tctgtttcgc aaatgcagat gatcgaaatc gccaaagcgt
    30781 tttcctataa cgcgaaaatt gtgattatgg atgaaccgac ttcttcgtta accgaaaaag
    30841 aggtcaatca tctgttcact attattcgta aattaaaaga gcgcggctgc ggtattgttt
    30901 atatctcgca taaaatggaa gaaatcttcc agttatgtga tgaagttacc gtattgcgcg
    30961 acggtcagtg gatcgccacc gaaccgctgg cagggctgac gatggacaag attatcgcca
    31021 tgatggttgg gcgttctctt aaccagcgtt ttcctgacaa agaaaacaag ccgggcgaag
    31081 tcatcctcga ggtgcgtaac ctgacgtcac tgcgccagcc gtcgattcgc gatgtctcgt
    31141 ttgatctgca taaaggggag atcctcggta ttgccggtct ggtgggggcg aaacgtaccg
    31201 atattgttga gacgttattt ggtattcgcg agaaatcggc tggcaccatt acgttgcacg
    31261 gcaaaaagat caataaccat aatgccaacg aagccataaa ccacggattt gcactggtaa
    31321 ctgaggagcg ccgctcaacg ggaatttatg cctatctgga tattggtttt aactcgttaa
    31381 tttccaatat tcgcaactac aaaaataaag ttggtttact ggataactcg cggatgaaaa
    31441 gcgataccca gtgggtgatt gattcgatgc gggtaaaaac accggggcat cggacgcaaa
    31501 ttggttcgct ctcaggtggt aatcagcaaa aggtgattat tgggcgctgg ctactaacgc
    31561 aaccggaaat attaatgctc gatgaaccga cgcgcggtat tgatgtcggg gcgaagtttg
    31621 agatctatca gttaattgcc gaactggcga agaaaggcaa agggattatt attatctcct
    31681 ctgaaatgcc tgagttgtta gggataacag accgtattct ggtcatgagc aacggtctcg
    31741 tttccggaat tgtcgacaca aaaacaacaa cgcaaaacga aattctgcgt cttgcgtctt
    31801 tgcaccttta agattagggg cttcccatga gtgcgttaaa taagaaaagt tttcttactt
    31861 acctgaaaga gggcggtatt tacgtcgttc ttttagtttt gctggcgatt attattttcc
    31921 aggacccaac atttttaagt ctgttgaact taagtaatat tctcacccag tcatcggtgc
    31981 gtattattat cgcgctcggt gtggcagggt taattgtcac ccaggggacc gacctttctg
    32041 caggtcgcca ggtggggctg gcggcagtag tagcggcgac gttattgcag tccatggata
    32101 acgccaacaa agtgttcccg gaaatggcga cgatgccgat tgcgctggtt attctgattg
    32161 tctgtgccat tggtgcggtg atcggtttga tcaacggtct gattatcgcc tatctcaacg
    32221 tgacgccgtt cattaccacg ctcggcacga tgatcatcgt ctatggcatc aactcgcttt
    32281 attacgactt tgtcggggcg tcgccaattt ctggttttga cagtggcttc tctacctttg
    32341 ctcagggctt tgtcgcgctg gggagtttcc gtctctctta catcaccttc tacgcgttga
    32401 ttgcggtggc gttcgtctgg gtgttgtgga acaaaacccg cttcggtaag aacatttttg
    32461 ccattggcgg taacccggaa gcggcaaaag tatctggtgt caacgtcggc ctgaacctgc
    32521 tgatgatcta cgcgttgtct ggcgtgttct atgcctttgg cgggatgtta gaagccggac
    32581 gtatcggctc tgccaccaac aacctcggct ttatgtatga actggatgct atcgcggcgt
    32641 gcgtggtagg cggtgtatcg ttcagcggcg gtgtggggac ggtgattggc gtggtgaccg
    32701 gggtaattat ttttaccgtc atcaactatg gcctgacgta catcggcgta aacccatact
    32761 ggcagtacat catcaaaggg gcgattatta tcttcgccgt agcgctggat tcactgaagt
    32821 acgcgcgtaa gaagtgaaat gattgtggtc cgcattcggt cataaaggca cataacatca
    32881 tgatctgtgt ggttttgtag gccggataag gcgttcatgc cgcatccggc gacaggagcc
    32941 aaatgccgga tgcaacgctg gcgcgtttta tccggcctac aatgagctca acatactgat
    33001 ttgtatggtt ttgtaggccg gataaggcgt tcaagccgca tccggcgaca ggaaccaaat
    33061 gccggatgcg tctctggttt tacaacgtta ccgggtgttc tttctcgcct ttcttaaact
    33121 tcacttcccc gagctcaata caacccaccg ggcagacgtg accacaaagc agacaaccca
    33181 cacatttctc ggtattacaa tgcggtgtgc gggttttctc gctccattcc atcgcctggt
    33241 gaccgccgtc gtaacaggaa atataacagc gtccacagcc aacacattta tcaagattga
    33301 tacggggata gacaatataa ctgcggtcta aatcttccgc cgggacaata ttgttattcg
    33361 ccagacctac catctcctgc aacgaatcaa atccctgatc ggcgagataa tgcgataacc
    33421 cgctcgccat atcttctacg atgcgatagc cgtactgcat aatgccggtg gtgacctgta
    33481 acgttgctgc gccgagcaat aaaaactccg ccgcatcctc ccaggtttca atgccgccga
    33541 taccgctgat tgggaaatcg cgcagttctg gatgggtgcg catttgctga atgaagcgca
    33601 gggcgatcgg ttttaccgct ttaccggaat atccggaaat actcgatttt ccgttaacga
    33661 tcggcatacc gattttctga ttaagatcga tattggtgat ggatttaaca gtgttaatcg
    33721 ccgcaatgcc atctgcgccg ccgcgcttcg ccgccagcgc cacttcgcac atatcaccga
    33781 tattcggcgt catcttcgcc aacatcggca gcgtggaacc ccgtttcact gcccgacaat
    33841 atttttctac cagctccggg ctttgcccga catcgctacc catcgcatga gaagtcattt
    33901 gcggacagga gaagttacac tcgatcatat cggcgccagc ttcttgcacc agacgcgcca
    33961 gctcctccca ttgctgctca ttttccccca tgatcgaagc gatcaatact ttgtccgggt
    34021 aatcttcctt cagccgacgc agggcggcca gattctcttc caacggatgc tcggcaatct
    34081 gctccatatt tttgaagccg ataaaaccgg tatcttcttt caccagatga tcaaaacgcg
    34141 gcgagacttc gttggcgata aaaaagccga tcgttttaaa caccacgccg ccccaacctg
    34201 tgtcgtaggc tttggcacac atctcatagc agttgcctac cggcgaagaa gagaggcaga
    34261 acgggttggg aaacttcacg ccgcaaaaag taatcgaaag atctttcgtt aacatgagca
    34321 agctccctct aaatagtgat gaatcgcctc ggcggcttct ttcccggttt tcacggcata
    34381 gaccacggtt ttgtcaccct ctacaatatc gccagcagca aagacttgcg gatcgcgggt
    34441 ctgataattt tgtgttttga tggtgttacg ctgcggctct aactccgcaa agacatccag
    34501 tctggcatgc tgaccgacgg cgagaataat tttatctgcc gccatcgtca gttcgcccga
    34561 taaccgtaca tgcttaaacg tgactttatt gccttccacg gctactggcg tgaatccatc
    34621 gatgatcgaa acgcccagct cccgggcgct ggcaaactct ttttcgcttg ccggaaactc
    34681 atctaactct tcacgcgcta cgcaagttac cgcctgacag ccgagaactt tcagcgtgct
    34741 ggctacgtcc atcgcgacat caccgccgcc gataattaat gcgctttgtg gaatgctgat
    34801 atcgccttgc gcctgtcgtg cacgttgcag gaagtcgacg gcaatctcaa cgtcgctatg
    34861 ctcaaacagc ggtagcccgg aaccgcttga taaccccacg gtgaccagta ccgcgcggtt
    34921 ttccgctttc agttgctcca gggtgagtgt tttaccgatt tcgttattgc acttaatggt
    34981 cacgcccatt ttttcaatac gggcgatctc tgcatccagc actgattgcg gtaaccggaa
    35041 ttgcggaata ccgttacgca accagccacc ggggtgcgct tctttctcat aaatcgtgac
    35101 gtcataaccc tggtttgtca gtgtcacact ggcctgcaat ccggcaggac ctgcgccaat
    35161 aatcgcgact ttgccgagca ttttagtacc gggctgataa atttccatcc cggtttgttg
    35221 ttcaaaatca gtaacaaaac gctgtaagcg gccgatatca atgggagcat caacaccggc
    35281 acgggtacaa ccgctttggc ataatttctc cgtcgggcac actctggcac aaacggcacc
    35341 gagggcatta ttttcgcgaa ttgtctcggc agcgccttta aaattacgaa agtagattga
    35401 gcgaataaat ttccccggat cggtctgtgc cgggcaagcc tgactacagg gagcgtcgtg
    35461 acataataaa cagcgagagg cttctttaat cgccagtaac ggcgtaaaag ccggagtgag
    35521 ttcatccaga taattttgtt gcggcatgtg atcttcctta atgctatgag taattaaggc
    35581 gaacgctaaa taaatattgc gtgctgatat taaggctatg cgcgtgggga aatttaaact
    35641 gtgaaaggat tcacaaatag ctgcggttca gaatgttata tccgcagctt attgagtgaa
    35701 gtaataatta ttctcaataa ttaacagaaa agagaaaata ttaacgatag aaaataagat
    35761 aaacaatcat gccgatagat aaaattccga agccgatata tgccaccata taagcgaaaa
    35821 agagtccggg attttttgct cgccagacat acaccggttc cggtgcattt ttaacccgtt
    35881 tatcaatcgc gcctgcgcgc cagcctttcc atgcttcgcg gaaacaaaat atcgggataa
    35941 ggaaaaaaac ggcaacgaca aaaaactgct gtacatccat aactactttc cttgtttctt
    36001 ttgtaattca agtaactgtt cgggtgtgac ggcgggatac ccctgcgcat cttccggcac
    36061 ctggtgcata gccggaatag ggaccagcgg ccctaaaaaa cgcggttcac gtttaaaaat
    36121 ataaaggtca gccagcgcac cgaaacgggc ggcaaattcg cgaatacgta ctgacagcat
    36181 atctttcggt gacggtacgg cataacactg agcctgaatc cccatatgca gcgcaataaa
    36241 taatgcccgc tcacagtgga aacgttgggt gataataatg aaatcattag tatcgaaaac
    36301 tttgcgtgta cgcactatgg aatccagcgt acgaaagcct gcgtaatcga gaacaatatc
    36361 tgatgggtcg acaccagcag cgattaaatc tttgcgcatg gtcatcggct cattataact
    36421 ttgcaatgcg ttatcgccgc tcagtaatag ataatttacc ttaccgctgt tataggcatt
    36481 aatcgctcct tgaatgcggt agcgataata ctgattaatt acgccagtac gataatattt
    36541 tgctgttccg agcaccacac cgacctggcg gtaggggaga tcctgcaatt cgtcgtagat
    36601 ataaggcgcg gttttccagc tcatccagcg atcgaggccg agcacagtca acagcagcaa
    36661 gccgataagg actaacaggc tgaggaacac gcgctttaac atgcaggaga ctcgatctaa
    36721 ggaaaatttc catcaggcta ctttacccgc tggttttgcg caagaaacgg caaactgttt
    36781 gccgggattt tcgacaccag gagagggctg gcaagccgga caaggcgttc acgccgcatc
    36841 cggcaccagg cttaagcgag aagcacgcgg tcgatactgt gacagccgag cgctttcagc
    36901 gttgcggagg tggcatccca ctgaatcaac ggcgcatcgg ctttttctgc cagaaccgcg
    36961 cgctggatat ccgggtaatc ataacccttg agattcaaca gaattaacgc tccctgcaac
    37021 ggcggcagag tcgggttgaa tgcggcgttt tcagcgtagc tgccgctgaa aatacgaccg
    37081 tctttacatt ccagcgcgac accgcttggc gacttactgt aaggcatgtg cgaacggttt
    37141 gccgccgcaa tcgccgcctg agaaagcgca tcaccagtca gcgcatagcc gtgatcctgc
    37201 tcgtccatca gcagcgtttt aatctccaga tctttcggcc caaaggcatc cggcagatag
    37261 tcacgcagct cgtgtgcctc gcggcccggc agatgaatac gcagatccag accgctgttc
    37321 agttcattca taaactgacg gcagtgacca caaggcgtgt agttaacggt gatggctgca
    37381 agcgcttttt caccactcaa ccaggcgtgg ctgatcgcgc tttgttcggc atgaacggtt
    37441 tgctgcattg tcgcaccaat aaattccata ttggcaccaa aataccaggt tccgctcaca
    37501 ccgcgcgcaa ttgcgccaac attaaaattc gacaatggcg tacgcgcaca ggccgccgcc
    37561 agcggaagta gtgcgaatgc cagcgcgtct tcgtccagcc ccgttgcgct cttcagcgat
    37621 gagacttgct ccccggtcaa caaagcgggg aagtacttgt ctgccagaat aggttccagt
    37681 gcagattgca agttatccgc aagttgggca aaagcggttt gaaaacgtgg atgcatggcg
    37741 ttgcctcata atcatgtaat gggataacag tttacgaacc cgttatggct ttatatgtga
    37801 tctgaatctc attaattatg caacgcgtga aacttacatc aaaaatgcgc gacgcatcgc
    37861 aaattttagc ccattactgc cagaataatc gggaaaagga acggtgcgat cagcgaagta
    37921 attatcccgc ataacaccag cgccagcgaa ctaaatgcac cttcctgata atccagctcg
    37981 gcgcagcgcg ccgtaccgag ggcgtgcgag gcagtcccca ttgccagacc gcgcgcagct
    38041 ttggtacgaa tacgcatcgc attaagcaat gtatggccaa ataccgcgcc gaggatgccg
    38101 acgaaaatca cgcaaactgc gctgattgcc ggaataccgc caatactgcc gccaaccgcc
    38161 attgcaatag gcgtggtgac tgatttcggc aggattgacg cggcgatttc cggtgaagcg
    38221 cccatcaaca atgccacgga agtccccgtc accatcgcaa ccacgctgcc gataaaacag
    38281 atggtgatga tcgatttcca gcgcgcgcgg atctggtgta gctgctcata taaaggatag
    38341 gccagcgcga cgaccgctgg ttgcagcaga tcgtttagca cttcgctacc tttaaagtaa
    38401 ctgtcgtaag agatgccggt cagcattaaa aaaggaatga tcaccaccat tgctaccagc
    38461 aacgggttaa gcaggggaaa cttataccgt gccgccagtt tgcgggcggc aaaaaagaca
    38521 atcaacgtca gtggtaatga ccaccagata ttcgccatca ttcttctgat cctttctgac
    38581 ctacgacttt acgttcaccg tgtaccagtt gcgaactcca gctcaccacc agaaaaacca
    38641 ccagcgtact gattgcacag gaaaccacta ccgggccaaa ctgtgcgcgg agcaaatcaa
    38701 aatattgcat gacgcctacg ccaatcggta caaacaatag cgccatatag cgaatcagta
    38761 cgtagcaccc cggattgacc catttcgccg gaagaatttg caaggccagc aggacaaaca
    38821 ggatcagcat cccgatgatg ctgcccggaa tggttaccgg tagcagggaa gcaatgaaaa
    38881 tgcctgcata caggcaggca taaatcagga cgaaagcgcg taaatattgc cagataatgt
    38941 tcagtgtctt actcataatc aacaccccag atgagatgga ttcatcatac aattaattcg
    39001 taaaacgtgc taccgatcac attaagaaat cagcgcttat tttttcgcac cccggtataa
    39061 cgcaatgagc agcgaactca ggcatacatc aagcatagag agggattgca gtttgcagca
    39121 atccctcttt gcagaccttt aatccgctga ccataggcgc taacatagcg cctcattttt
    39181 ttgcgggtga tgatatgcgt gtgttactgg caccgatgga gggagtgctt gactctctgg
    39241 tgcgtgaatt gctgaccgaa gttaacgact acgatctgtg catcaccgag tttgtccgcg
    39301 tggtagatca actgctgccg gtaaaagtct ttcatcgcat ttgccctgag ctacaaaacg
    39361 ccagccggac accttccggt acgctggtgc gcgtgcagtt gttaggtcag ttcccacaat
    39421 ggctggcaga gaacgccgcc cgtgcggtgg aattaggttc ctggggcgtg gatctcaatt
    39481 gcggctgccc gtcgaaaacg gtcaacggta gcggtggtgg ggcgacgtta ctcaaagatc
    39541 ctgaactcat ctaccagggt gcaaaagcga tgcgtgaagc tgtaccggcg catttgcccg
    39601 tcagcgtgaa agtgcgtctg ggctgggaca gcggtgagaa gaaatttgaa atcgccgatg
    39661 cggttcaaca ggctggcgct acggagctgg tggtgcatgg gcggacaaaa gagcagggtt
    39721 accgcgcgga gcatattgac tggcaggcga ttggcgagat tcgccagcag ctgaatattc
    39781 cggtgattgc caacggtgaa atctgggact ggcagagtgc gcaacaatgc atggcgatca
    39841 gcggctgcga cgcagtgatg attggtcgcg gggcgctcaa tattcccaac ctgagccggg
    39901 tagtaaaata taacgaaccg cgaatgccgt ggccggaggt ggttgctttg ctgcaaaaat
    39961 atacccgtct ggaaaagcag ggcgataccg ggttatatca cgtagcgcgg attaaacagt
    40021 ggttgggtta tttgcgtaaa gaatacgatg aagcaacgga attatttcag catgttcggg
    40081 tgttgaataa ttcccctgat attgcaaggg ctattcaggc aattgatatc gataagctgc
    40141 gataatagtg gaatcgcagt ttatcagaga caatgaatcg cttattcagc ttcatcatct
    40201 tgttgcgctc ctttcatgag ctaagccaca tattgccact ggcgcaagga gcgcgcaggg
    40261 ggaggacaat cgcctcctcc ccctgcaccc ccgggctctg gcgaacaaaa tcgccgctgc
    40321 gcggtacctt cagcttatcc ccttcggcta ccgggtcggg cgcgaggtaa cgtccttgta
    40381 agacgcgccc tcagcccaca tccatgtggg ctgccccggc cttcggggaa cgcttcagcg
    40441 attttgacgc caccaacacc ggagctgttt gcaaatcaag tacacaaaat atctagttcc
    40501 tgacctgaca acttatatgc catatctgat ttttaaaata tcatcttaaa tacgccagtc
    40561 accaccagta gccc
//
LOCUS       DAPGOF010000020        39447 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.020, whole genome shotgun sequence.
ACCESSION   DAPGOF010000020 DAPGOF010000000
VERSION     DAPGOF010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.020"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(31..1890)
                     /gene="gss"
                     /locus_tag="RHV13_000936"
     CDS             complement(31..1890)
                     /gene="gss"
                     /locus_tag="RHV13_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HDV1240491.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKE"
     gene            2095..2967
                     /gene="yghU"
                     /locus_tag="RHV13_000937"
     CDS             2095..2967
                     /gene="yghU"
                     /locus_tag="RHV13_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HDV1240492.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRRGKG"
     gene            complement(3083..3331)
                     /gene="hybG"
                     /locus_tag="RHV13_000938"
     CDS             complement(3083..3331)
                     /gene="hybG"
                     /locus_tag="RHV13_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HDV1240493.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3344..3685)
                     /gene="hypA"
                     /locus_tag="RHV13_000939"
     CDS             complement(3344..3685)
                     /gene="hypA"
                     /locus_tag="RHV13_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HDV1240494.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3678..4166)
                     /gene="hybE"
                     /locus_tag="RHV13_000940"
     CDS             complement(3678..4166)
                     /gene="hybE"
                     /locus_tag="RHV13_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HDV1240495.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4159..4653)
                     /locus_tag="RHV13_000941"
     CDS             complement(4159..4653)
                     /locus_tag="RHV13_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HDV1240496.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKSAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4653..6356)
                     /gene="hybC"
                     /locus_tag="RHV13_000942"
     CDS             complement(4653..6356)
                     /gene="hybC"
                     /locus_tag="RHV13_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HDV1240497.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6353..7531)
                     /gene="hybB"
                     /locus_tag="RHV13_000943"
     CDS             complement(6353..7531)
                     /gene="hybB"
                     /locus_tag="RHV13_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HDV1240498.1"
                     /translation="MSHDPKPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7521..8507)
                     /gene="hybA"
                     /locus_tag="RHV13_000944"
     CDS             complement(7521..8507)
                     /gene="hybA"
                     /locus_tag="RHV13_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HDV1240499.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8510..9628)
                     /gene="hybO"
                     /locus_tag="RHV13_000945"
     CDS             complement(8510..9628)
                     /gene="hybO"
                     /locus_tag="RHV13_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HDV1240500.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9817..10104)
                     /locus_tag="RHV13_000946"
     CDS             complement(9817..10104)
                     /locus_tag="RHV13_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HDV1240501.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10223..11110)
                     /locus_tag="RHV13_000947"
     CDS             complement(10223..11110)
                     /locus_tag="RHV13_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HDV1240502.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAIGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGDVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11267..12307
                     /gene="gpr"
                     /locus_tag="RHV13_000948"
     CDS             11267..12307
                     /gene="gpr"
                     /locus_tag="RHV13_000948"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HDV1240503.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPEDSRMHREGNKVRGLSPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12347..12841)
                     /locus_tag="RHV13_000949"
     CDS             complement(12347..12841)
                     /locus_tag="RHV13_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HDV1240504.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13032..13916
                     /gene="yghA"
                     /locus_tag="RHV13_000950"
     CDS             13032..13916
                     /gene="yghA"
                     /locus_tag="RHV13_000950"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HDV1240505.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPMLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14188..14613)
                     /gene="exbD"
                     /locus_tag="RHV13_000951"
     CDS             complement(14188..14613)
                     /gene="exbD"
                     /locus_tag="RHV13_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HDV1240506.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14620..15354)
                     /gene="exbB"
                     /locus_tag="RHV13_000952"
     CDS             complement(14620..15354)
                     /gene="exbB"
                     /locus_tag="RHV13_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HDV1240507.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15606..16793
                     /gene="metC"
                     /locus_tag="RHV13_000953"
     CDS             15606..16793
                     /gene="metC"
                     /locus_tag="RHV13_000953"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HDV1240508.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHKFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16933..17592
                     /locus_tag="RHV13_000954"
     CDS             16933..17592
                     /locus_tag="RHV13_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDV1240509.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17632..18531)
                     /gene="yqhC"
                     /locus_tag="RHV13_000955"
     CDS             complement(17632..18531)
                     /gene="yqhC"
                     /locus_tag="RHV13_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HDV1240510.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILHLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18725..19888
                     /gene="yqhD"
                     /locus_tag="RHV13_000956"
     CDS             18725..19888
                     /gene="yqhD"
                     /locus_tag="RHV13_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HDV1240511.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            19993..20820
                     /gene="dkgA"
                     /locus_tag="RHV13_000957"
     CDS             19993..20820
                     /gene="dkgA"
                     /locus_tag="RHV13_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HDV1240512.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLIHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            21020..21946
                     /locus_tag="RHV13_000958"
     CDS             21020..21946
                     /locus_tag="RHV13_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HDV1240513.1"
                     /translation="MKIILLFLAALASFTVHAQPPLQTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKAANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            21995..22252
                     /gene="yqhH"
                     /locus_tag="RHV13_000959"
     CDS             21995..22252
                     /gene="yqhH"
                     /locus_tag="RHV13_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HDV1240514.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNVKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22295..24514)
                     /locus_tag="RHV13_000960"
     CDS             complement(22295..24514)
                     /locus_tag="RHV13_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HDV1240515.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24625..26037)
                     /gene="ftsP"
                     /locus_tag="RHV13_000961"
     CDS             complement(24625..26037)
                     /gene="ftsP"
                     /locus_tag="RHV13_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HDV1240516.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(26112..26849)
                     /gene="plsC"
                     /locus_tag="RHV13_000962"
                     /gene_synonym="parF"
     CDS             complement(26112..26849)
                     /gene="plsC"
                     /locus_tag="RHV13_000962"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HDV1240517.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(27083..29341)
                     /gene="parC"
                     /locus_tag="RHV13_000963"
     CDS             complement(27083..29341)
                     /gene="parC"
                     /locus_tag="RHV13_000963"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDV1240518.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(29887..30369)
                     /locus_tag="RHV13_000964"
     CDS             complement(29887..30369)
                     /locus_tag="RHV13_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDV1240519.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(30422..30814)
                     /gene="ygiW"
                     /locus_tag="RHV13_000965"
     CDS             complement(30422..30814)
                     /gene="ygiW"
                     /locus_tag="RHV13_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HDV1240520.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            30966..31625
                     /gene="qseB"
                     /locus_tag="RHV13_000966"
     CDS             30966..31625
                     /gene="qseB"
                     /locus_tag="RHV13_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HDV1240521.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQCEPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            31622..32971
                     /gene="qseC"
                     /locus_tag="RHV13_000967"
     CDS             31622..32971
                     /gene="qseC"
                     /locus_tag="RHV13_000967"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HDV1240522.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHYGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAMVSW"
     gene            complement(33022..33351)
                     /locus_tag="RHV13_000968"
     CDS             complement(33022..33351)
                     /locus_tag="RHV13_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HDV1240523.1"
                     /translation="MLKHKIQTIFEALLYIMLTYWLIDAFFAFNKYDWMLDSGDNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIIILRKLFVREMFEFWLYVCSFVICLVCGWWLFW
                     GRFIFCY"
     gene            33709..34290
                     /gene="mdaB"
                     /locus_tag="RHV13_000969"
     CDS             33709..34290
                     /gene="mdaB"
                     /locus_tag="RHV13_000969"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HDV1240524.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            34321..34635
                     /locus_tag="RHV13_000970"
     CDS             34321..34635
                     /locus_tag="RHV13_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDV1240525.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(34680..35567)
                     /locus_tag="RHV13_000971"
     CDS             complement(34680..35567)
                     /locus_tag="RHV13_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDV1240526.1"
                     /translation="MIQKDKTRVDIYGERFRTRASQLTPGLRAVASYINEHREVVLEQ
                     TAMEIAATLNTSDATVIRAIQALGFAGLRDLKRTLEQWLGPALSSSEKMSTTVSNLTS
                     DVNTAIDFVLEGHLYTCNVLSEPENRHALAQAVALLVQARQVAIFGIGASGILADYTA
                     RLFNRIGLPATALNRTGIGLAEQLIALQRGDVLIMMAQKSAHREGLTTLREARRLGIP
                     VILLTNALDSRFSKDASIVIHVPRGDEKGKTPLHGTVLLCLEMIVWSVASAVPQRAVK
                     TIKRINDFHRGLKTGRKNG"
     gene            complement(35564..36511)
                     /locus_tag="RHV13_000972"
     CDS             complement(35564..36511)
                     /locus_tag="RHV13_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HDV1240527.1"
                     /translation="MRLFFSLLILLSFFARATEPVQVFTDDLGRKVTVPAHPKRIVSL
                     HDLDITIPLIELGVPPVASHGRTRPDGSHFIRSGALLTGVDFDNSSIAFIGTADIDIE
                     AIVAAKPDLIITEPTRNTPIEQLEKIAPTVSIDHLKGGAPEIYRKLAELTGTQSQLAI
                     LERRYQAQINALKATLDSQKITVSVIQANQGKINVMHSYHSLGRVLRDAGFRFPPLIE
                     SIPEGGRMDVSAERLPELDADFVFATWRGDTGGKPQDELAAMEKVMPGWCQFLTACRS
                     GRYVLISREEAISNSFASLGLMAAQIQSQIAGRPLPEAK"
     gene            complement(36522..37559)
                     /locus_tag="RHV13_000973"
     CDS             complement(36522..37559)
                     /locus_tag="RHV13_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDV1240528.1"
                     /translation="MNVGLRPLRVGKFSTLVRPKNLVLLGGLFLFAVGILIFGLMHGS
                     FSVSASEVGRALFAPENVSTDARYIVQDIRLPRVIMALLCGAMLGMAGAAMQSIARNG
                     LADPGLIGVKEGCSVAVLWLIFQFPMLGMFWRPVAGLAGGLLVALIVIFCARDISRPR
                     FVLIGIGVSWFFAAGIGVFMTTADVRDVQTALMWLSGSLHAANWMLVGISACWMLPAA
                     LLLLFTARTADIALLGHQVATGLGVNSSRLALLRVAAPIILTAVCVSCVGNIGFVGLI
                     APHISRFILRGGQTTLLLGSAVSGALLVILADSIGRLAFLPLQLPAGIIISLIGGPFF
                     LLLLWQRRNSF"
     gene            complement(37568..38551)
                     /locus_tag="RHV13_000974"
     CDS             complement(37568..38551)
                     /locus_tag="RHV13_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDV1240529.1"
                     /translation="MMRTGLVTVIFLTLLLVGCVVYPGIGARFIAPQTVLQAFLHFDP
                     QNFDHNVIVRLRLPRLAAALLTGASLGVAGALLQAVIRNPLGEPHILGLNAGAALAVV
                     AASALGLAFPAGRPLLASTGGALLFLLILLLSSAGRSGLTPMKVTLCGVALSAFVSSI
                     TAAILILDEQTLLAMRTWLAGDLAGQDWVTLGTSAWFSLGGFVLAIYLAPSLNMLALG
                     DRMAQGLGVSVLRTRTFTLLAIALLCGAAVSIAGPIGFVGLLVPQIVRRLVSADLRVL
                     LPLSACVGALLLLLADIIARTLFTPHELATGVMTALVGAPVFVIMATRMFK"
     gene            complement(38548..39357)
                     /locus_tag="RHV13_000975"
     CDS             complement(38548..39357)
                     /locus_tag="RHV13_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV1240530.1"
                     /translation="MAHNAKGQGLILDNVSAGYHKKIIVDGVSFSVPTEKMTVLVGAN
                     GCGKSTLLSTIARILQPMGGSILLDGKAIHEQPTKALSRRLGILPQSPLLPEGLTVYE
                     LVSRGRFPWQNFIRQWSDADEQAVEEALKLTGTQEFAHLPVEKLSGGQRQRCWIAMVL
                     AQKTPYILLDEPTTWLDLRYQVEILELLHDLTRHHGRTVVVVLHDLNFAVNYGDTLIF
                     LRQGKVVRVLNEVEHCTPELVKAVFDVDVHASINPLTGKPFFMPFRGVEKV"
BASE COUNT         9925 a        10575 c         9834 g         9113 t
ORIGIN      
        1 ataatcagta cgggattgct acaacatgta ttactctttc accacaatta acggttcaat
       61 atcactctct tttttaataa ccagtgattc atcaccgcgc aaacacgtcc caccgtagtt
      121 gccgccaacg gtgaaggtac atacctgaat gtatttaccg tccactttcg gcaaacacca
      181 cagttgctga tagatgtttt tctgctcggc aaatttacca ctggttttgt ccagcacctc
      241 ttcatgatgg ctgacgaggt cgatgttact gccacagcga ccggcgatcg gtttcactgc
      301 gtaacctgtt ttcaccaatt catcattaac agtgaaatcg gtatccagca ggtaacgatg
      361 gtgcgggaac agcgaccaga ggatcggcag aatcgctttg ttgccgggga tcaccgtcca
      421 cagcggctca aagaccagca cttccgggcg cagcaatacg tcgataagac gcacttcatt
      481 ttgcggatga ccggtacgga tcggcaccgc agcaaactca cggtcgctaa cttcacgaat
      541 ctgatcaaac gcggtttccc acgcccaggt tttccacacg cagttaacca gtcgcccttc
      601 cccatcaatc agttgcccgg cagcatccca gcccagttca tccagcccgc gcaagatacg
      661 cgtttcaaag cccgcctggt gcagcgcctg ctccataaac tgcgcgtgat agttttcctc
      721 gatatctttg tcctgcatga tatggacaaa cggacgtgca cgactgtgtt tccaggcacc
      781 agccagttcg ttaatcagcc cttccgccgg attgaagccg ttgcctttat agccctgctc
      841 cgcccaacgt tcgaggatca agcccgcttc ggtatgacat gaggcggagt cggcgttgta
      901 ctcgtaaacc ttcagaccac gctcatccat gcagaaatcc atacgaccgg tgatcatatg
      961 gtgacggcga cgctgccagg agagacgcaa acgtggccag aggattttcg gaatgtcgaa
     1021 cagcgccagc aggttgtcat ctttcagcac cttgtcggtt gcgtgaagat acatcaggtg
     1081 cagctcgttg gtggctttaa tcagctcctg ctcggcgctc tcggtaatgg tgtagtaatg
     1141 atacggatcc tgattgatca cctgaccgtt ggcctgcaca taggcgtttt gcagcggatc
     1201 tttttcatcc agccatttac cgtcaaactg gcctttgttt tccagacgcg ctccgctgat
     1261 tttcagcagc tcgcctgcaa tttccggctg cggtaagctg tattcagtat cttccgtctg
     1321 gatcatccag cccagaatgg tggtgtcatc aaaagtgtct ttaagggtat agcagccgtt
     1381 ttcgaccacc atctccagtt cgcgcgtcca ctgttgccct tgcggcaacg gggaatgaat
     1441 cacgttctgt tccgcaatac ggactttgtt gccatgcaat tgggtaatga tggcgacatg
     1501 gccggtgtct ttaaattcac cgcctttatc ccagataaga agcgcacccg cgaccggcgc
     1561 acgcggcgag ccgttaggaa atgcctgcaa tggcaggatg ttgtcattaa ccacttcacg
     1621 caggaagcgc agcgagaaaa tctcccacgc catacccacg tcagtaaaga ccacaccgta
     1681 gttcagaaag agaaaacggc gagcaaattc aacgcattgc catttgtggc ccatatattc
     1741 gtcgtcgata tagctacgga atacggcgtc atcttcgtat tcctgcggat cgagagaact
     1801 gtaatctgaa gagtagattg ctaccccacc tggggcgtag cccaataatg tcccgaacgg
     1861 ggcatcctgg ctggtcgttc ctttgctcat cactttacct ttaacaatac aacctaagca
     1921 gttggcgtaa ttgttgtgct ctgattacct gctcagcgac attaaccgga cagaggtcag
     1981 actaatcata cacctcatcg cagcggctgg cgcaaagggt taaaaaattc acattctgta
     2041 cagcaagtga cctgctacac tgcttcaaca ctaccactca gaaggcaact cactatgaca
     2101 gacaatactt atcagcccgc gaaagtctgg acgtgggata aatccgctgg cggcgcgttc
     2161 gccaatatca atcgcccggt ttctggtccg acgcatgaaa aaacgctgcc cgttggcaaa
     2221 catccattgc aactttattc gctgggaacg ccgaacggtc agaaagtaac gattatgctt
     2281 gaggagctgc tggcgctggg cgttactggt gcagagtacg atgcctggct gatacgcata
     2341 ggcgatggcg atcaattctc cagcggcttt gtcgaagtga acccaaattc gaagatcccg
     2401 gcgctgcgcg atcataccca taatccgccg atccgcgtgt ttgagtcagg atcgattctg
     2461 ctttatctgg cggagaaatt tggctacttc ctgccgcagg atctggcaaa acgtactgaa
     2521 acgatgaact ggctgttctg gttacagggc gcggcaccgt tcctcggcgg tggttttggt
     2581 cacttttacc attacgcgcc ggtgaaaatt gagtacgcca tcaatcgctt taccatggaa
     2641 gccaagcgcc ttctcgacgt gctggataag caactggcgc agcataagtt tgttgcaggc
     2701 gatgagtaca ccattgcgga tatggcgatt tggccgtggt ttggcaacgt ggtattaggc
     2761 ggcgtatatg atgccgcaga gtttcttgat gcgggcagtt ataagcatgt gcaacgctgg
     2821 gcaaaagaag tgggcgaacg tccggcggtg aaacgtgggc gtattgttaa ccgcaccaac
     2881 gggccgctga acgagcagtt gcatgagcgc catgacgcca gtgattttga gacgaatacg
     2941 gaagataaac gtcggggtaa gggttagcgt tcgtcgcagc aagccatcca ggccggataa
     3001 ggcgttcacg ccgcatccgg caatcgagca caatgcctga tgcgacgctg ccgcgtctta
     3061 tcagacctac gaaagctaat catcacgcgc tggtaatgtc gtaatccatt tggcgcagtg
     3121 cgtctaatgt ggctttggct tcatcttcgt cgatgatgct catggcaaat ccgacgtgta
     3181 ccagcaccca ctggcccagc agatcggcag ggttaccttc acaaatcagg gcgatattca
     3241 cgtcgcgctt gataccacat acttcaacct gcgcaagctg gtgaatatct tcaccgacag
     3301 ccagcacctg gcctggaacg ccaatacaca ttgttaactc cggttattca acttcaatac
     3361 ttttgacgat cagcgaatcg ccggtatcga cacgcaaccg ctcgccgtga cagagcggac
     3421 actgcgcatc gtgctgatga atctccacca cctggctgca atcccagcac caggcctggg
     3481 cgggtttata gacgatatgt aaatcgcacc cttgcgccac cgttccgtgg cagacaattt
     3541 caaaactaaa acggacggcg ctctcctcaa cgcaggagag cgcgccaatt tccagccaca
     3601 cggcggtgac gcgcttaaca tcgtgctgct ccgcctgccg ttggataatt tcaacggcac
     3661 tctggcaaag cgacaactca tgcattttcg ccactcctgc gaccaaacag cagggcgcga
     3721 cgccctgcgt gtggaacatc cggattcgtg acaggcagcg aaaggatcat tcgtgcgcag
     3781 tcatccgtca ggcgttggcc ctcttcaatc gacatactgt gcgaaagcgg tgacatcagc
     3841 gaacaggaga ggtattgcga aacaccgtcc agttcgccaa cggtaaaggt catggtgcca
     3901 tacggcagtt gcagaccaat tttttcactc actttgcgca gcggccagag ttgatccggg
     3961 ccggggaaga taactgcact cagcatccac ggggtgatca cacaccccgt ccactgacct
     4021 tcgaacagcg taaaatcaga aacatacacc ggcattgaag gatgcagaaa agagagatcg
     4081 tgcatcgaac gccgggcaat ttcttcaaac gctgcctgta cttgcgcctt cggggaggtc
     4141 tggaaacctg ctatctcttc agtcatgaat cgcctcccgt gggatagctt ccacgccaga
     4201 ttcacgcagc gcagccagaa cctgctcaag cgcaggttca atcatcgctt cgaccgtcgg
     4261 cgtcaagccg atgtgcggct ccagtgattc cggaattacg ccgaccaggg tcagcttttt
     4321 cggaaactcg ccggtgaagc gcagggccga caagacgtcg gccaggccaa gctgatgcgg
     4381 agagattttg ttggtaaaca acgccggaac ttcttcatcc cgcaggatca tcatcgttcc
     4441 cggcgcgctc ttcttcgaca caatggcatc cgcaataatc agatgatcgc gatttgccat
     4501 gtcgccaagc agctccattc ccgccgtgcc gccatcgagg atctcaacat aatccggcag
     4561 aatgtatcgt tgctctaacg cttcgacaat ccgcacaccg atggcttcat cggtcagcaa
     4621 aatattgccg acccctaaga ctaaaatacg cattacagaa ccttcactga aaccacttcg
     4681 ttgccgtcag catccactac gtgtaccgca caggccatgc acgggtcgaa ggagtgaatg
     4741 gtacgcacca cttccagcgg tttattcgga tcggcaaccg gtgtacccac cagcgactgc
     4801 tcgtaaggac cgacgtcatc attgaagtta cgcggaccag agttccaggt tgatggaaca
     4861 accgcctggt agttgctgat gataccgtct ttaataacca tccagtgaga gagcataccg
     4921 cgcggcgctt cgaggaagcc aacacctttg aactcacccg ttgccggaat gttcggcttc
     4981 acaaaggtgg tgtgatcgcc tttgccgata ttggtgatca gtgcactgta ttggttttgc
     5041 aggatatcct gcaattcaca gcagtgaacg gtacgaccaa taatacggcc cagcgtggag
     5101 tgcagctgtg ccacttccag cgtgttgcca gtcagtttct gataaatcgc aacgatttca
     5161 ttcagtttgt tctgggtaga ttcgcgacct gccgccagtt tcaccagcat attagccagc
     5221 ggacccactt ctaccgtttt gccgtagaaa gtcggcgatt tcacccagga atatttaccg
     5281 tcgtcagacc aaccgtcgta agccggaatg gtggtacctt cccacggtgc ctgcggcgct
     5341 tcgtctttat accaggagtg cttcgcgctt tcctgaatgc ctttgatcag gtattcatcg
     5401 gaatgagaag tgatcggacg atacgaggac agatccgcat tctcaatgta gccgcccggg
     5461 aacaggaagc tgccgttttt gctgtcggtc gggaattccg gcacgctcag gtagttcacc
     5521 gcacctttac cgcgcgtcag ccattccggg tagaacgcgg cgataaccgc ggtatcaacc
     5581 ttataaacct gttcaacaaa gtcgctcagc ttgtcgatga aagacttgat gtacatcagg
     5641 cgctcaaggt tcagcacgcc cagaccgtcg aggttgatcg ggttagcgac cccacctacc
     5701 gccaggttct gaatgtgcgg cgttttaccg cccagcagtg ccacgacgcg gttagcgtca
     5761 cgctggcact ccagcgcctg caggtagtgc gctaccgcaa tcaggttcac ttccggcggc
     5821 agtttcatcg ccggatgacc ccagtagccg ttggcgaaaa tgcccaactg accgctggca
     5881 accagatctt tgatcttgtt ctgaactttg gtgaactctt ccggactgtt cagatgccag
     5941 gtcgaaacgc ctttcagcat ttcggaggct ttggttgggt cagcttgcag tgcagaagtg
     6001 atgtccaccc agtccagcgc cgaaagctga tagaaatgaa cgatatggtc atgcgtggtg
     6061 tgcgcagcca gaatgatgtt acggatgtat tgcgcgttaa ccggaacgtc gatattcagc
     6121 gcactttctg ccgcacgaac ggaagacagc gcgtgagtgg tagtacatac gccacagata
     6181 cgttgcacaa tcatccatgc atcgcgcgga tcgcggtttt tcacgatctc ttccatgccg
     6241 cgccacatgg taccggaagc ccatgctttc gaaacgacgc cattttcgat ttcgcaatcg
     6301 atgcgtaaat gcccctcaat acgggttacc ggatcaatag taattctctg gctcatgctt
     6361 tgctcgcctc atgacgatta tgatcgtttt gttttaaagg aggaagtatc ggcagtagac
     6421 gaatgagtac gatgtaagcg caaatctcaa tagccacaaa accaatagaa atcaacagtt
     6481 cttcccaggt cgggaagtag gcgtaaccgc cgcccgggtt gaatgccacc agcgaatagg
     6541 tcagacgcca ggttgcacaa cctaacagtg cgctcagtgc tgacaggaac agcatgcggg
     6601 aatcattacg cagcttcgcc acacgcagaa cgaccagcgg gaagagcatc agcaggactt
     6661 caatccagaa catcacggag tagaagtcac cggcaaacgc taacgacagc ttgtcgcgat
     6721 agataagctc gccaaagcgc agcacgatga aaatcgccag caacacactg atggtgttgg
     6781 tcagcttgac gaacaggctc ttttcatccg gaccgttgcc acgcagaccc gcctgcacca
     6841 gcgaaccttc aaagatgaca atcgagaagc ccatgatgaa cgccgtcagc agcgagaaca
     6901 gcggcaacat ttcatagctc tgccacagcg gatgcacctt gtagcccgcc gagatcatca
     6961 gcgaccccat tgaagactgg tgcatggtcg gcagcagcgc accgagcgcg atgatgaaga
     7021 acatcacctt gttcagacgc tgtagcgaca ccttccagcc cagacgttca aacagtgccg
     7081 gagcaaactc cagagccatc acgccgatgt agatggtcat acagaccgcc gtctcgaaca
     7141 gtaccgagtt cacgttgaag tgacccggaa tgtagaagta cggcaggttc cagtagcgac
     7201 ccacgtcgat agtgatcgac aaaccaccca gtgagtaacc aaacagactc gccaacagcg
     7261 ccggacgcac cagcggatgg tattgcccac ggttaaagac gtataccgcc cacgccagcg
     7321 cccagccgcc acaggcaaag ccggtgccaa tcagcaggtc aaacgcgatc cacacgcccc
     7381 acgggaagcc gccgttcagg tcggagacag agcccagacc gaacaccaga cgcttcacaa
     7441 tcaggagcat acagatgacg attaacggtc caaaaatcat gaccggttta ctgatgattt
     7501 tgccgcccag cggttttgga tcatgactca tgatcgtctc ctccgtcgtg atggtcgttt
     7561 ttggtgttgc gacgaaccag cacggttaag cccgccagca cagccagtgg tagcatcatg
     7621 cctttataca gggtgtgttg aatattttcg gaacgcgcac cggtagaaag atcgtccagt
     7681 ttcggcaggt cgagattttc ataaggcaca cccgtcagta ccagaacctg agtaccgccg
     7741 ccctctttct cgccgtacag atgcggataa tatttcggca ccgtatgcag gtaagtgtcg
     7801 ccagatttca gcgtctgacg cggatagtgg tattcgctgc caggcttcag cgccagacgt
     7861 tttttcgcct ccgccatcag ctcttcacgc gtaccgaaaa tcaccgcgcc cgccgggcac
     7921 acttctacgc agccaggcag accgccttta tcgagacgtt ccacaccttt ctggttgcac
     7981 agctcgcact tatgcagcgc accaaacggg ttgttgtagt cgtacttcgg cacgttgtac
     8041 ggacaggcga ccatgcagta acggcaaccg gtgcacacgt ctttgtcgta atggacaatg
     8101 ccggttttcg gatctttttt cagcgcagag accgggcaca cagagacaca gttcggatcg
     8161 acgcagtgca tacactgttt cttaatgtac gcgtagccgt tctcctcctg gtctttgttg
     8221 acccctgtgc cgctggtcca cacctgaatg atgttattgg tatacggcga cagtttgtcg
     8281 ttgttcgacc aggtctgttc cccttgcggg ttacgttcag ggaagttgat atcctgacac
     8341 ttggtgacgc aagcctggca gcctacgcac aaggtcgagt cgtacaacat ccccagcgat
     8401 cccggaattg gcgggcggtt ttcagcagcc gcatgactga cagacggcag cgcgcccgtc
     8461 agcaatgccc cgcaggaggc tgctttaata aaattacgtc tgttcacggt tattctcccc
     8521 gtgagtcagc gttatctttc ttttgctgac gacccagttc acgcaccgcc atcacgctga
     8581 caccggcaac cagcccaacc acaccgccga gcaaaccaat agcgcctgca gagacgttgc
     8641 cgccctcttt agcgttaaca tccggtttct gtgaacgcgg agtctgattt tcgacgttgg
     8701 caagctgatg gatgccttta tggaagccga taccttcttc gttacagcca tagcacgggt
     8761 gaccaatcgc caccggccac acaccgccaa catcgcagaa ttgcagcgtt gagcaattgc
     8821 cgtaagtttc tggcccttta cagccgaggt ggtacaggca ccagccttcg cggtggcctt
     8881 catcaccgaa ctctttggca aaacgaccag catcgaagtg cgggcgacgt tcgcagtgtt
     8941 cgtgaatcag acggccatag gcgaaggtcg gacggttttt gtcatccagt ttcggcggtt
     9001 tgccgtaagt gatgatgtgc gcaacggtcg cgaggaagtt gtgcgggttc ggcgggcagc
     9061 ccggaatatt gataacggtt ttgcctggca gaacttcttg caggctgact gcgccagttg
     9121 ggttaactcc agctgcggca acaccgcccc acgcagagca ggaaccgata gcgataatgg
     9181 ctgctgcgcc ttccgccgct ttgcggatgt gatccacaat cggctcaccg gcaaccatgc
     9241 aataaatacc gttatctttt aatgggatgg aaccatccac cactaacaca tactgccctt
     9301 tgtacttctc gagagcgtta tgtttgttct cttcgacctg atgaccgaag gcggcggaaa
     9361 gcacttcgtg atactccaga gagatagtct ccagtacgag gttttctacc gttggatgcg
     9421 ttgcacgaag cagagattcc gtacaaccgg tgcactcctg cgcgccaatc cagataactg
     9481 gcggacgctg cgggttagta accgattcgg ccatctctgc agcggctttg ctacttaacc
     9541 ccatggtggc ggctaatgct gcacaaagct tcatgaaatc acgacggtta atgccgtgag
     9601 aatggatgag ggtgttatct ccagtcattt atagttattc cgttgcgaag acctggcata
     9661 tattttgcct caatcgcaaa atcaataatg cgatcgatgc gccatttacc acacatttat
     9721 tatggttatc gatatcatga tactgcggcg gggcgctaaa acgaagggaa gaagcattag
     9781 tgtaattaat taaagcaaga taatacgtat gtttgatcaa ttttcgaggc gataaccggc
     9841 cataaaaaag cgcaacgtag aacaggaatt attctcacga aagaaatcca ttaccatctc
     9901 tttatccagt ccatagcggc gcgtcagaat acccgcttcc caggcgctaa gctggcgatc
     9961 tccggtcttt tcgtacatcc gatgtgagta gcctaataca aaaccgcgtt tataatcggc
    10021 acagaatttt gtcacattaa ccgcactgtc ggcatgcgtt gcttttaatc ccgccattaa
    10081 gcctttacca aaatggttat tcatcgttca cctcattcgc tatatattgt catatatagc
    10141 gatattttag gcaaagaacg acttttgcgc acgctatttt ttcatgcttg tttaagacgt
    10201 aatcccgtaa gagataaacc tatcaggaga gatatttatc gaaccatttc agtgtccttt
    10261 gccaggcaag atcggcggca gatttgtcat aacggggcgt ggaatcatta tggaatccgt
    10321 gattaacccc cggatagata tacgcctcat aaaccttatt attggctttc aacgccgcct
    10381 cgtaagcagg ccagccctcg ttgattcggg tgtccagttc cgcgaagtgg agtagtaaag
    10441 gcgcttcaat cttcgccaca tcggcagtgg gtgcctgacg accataaaac ggcaccgcgc
    10501 aggccagttc cggatacgcg acagccgccg cgttcgatac gccaccgcca tagcaaaatc
    10561 cggtaatacc cactttgcct gtcgcttgcg gatagcgttg cataaactca attgcggcaa
    10621 agaaatcatt catcagtttg gttggatcaa cctgctgttg cagctcacga cctttatcat
    10681 cattacccgg ataaccgcca acggaactta agccgtcagg tgccagggcg atataccccg
    10741 ccttcgccac tcgccgtgcc acatcttcga tatacggatt cagtccacga ttctcatgca
    10801 ccaccaccac ggctggcgtt ttgccgctca tcttcgcggg cttcaccaga taaccacgta
    10861 cgtcaccgtg accatttggc gaaggatacg tgatgtactc agcaacaatc tccgggtcgg
    10921 taaactctac ctgagtcgcc agcgcataat ttggcttgag caaatcaaac aacgctaatg
    10981 ccgtcatccc gcctatcgca tacttcgccg caaggttgag gaactcacgt ttcgagattt
    11041 tcccgtgagc gtaatagtcg tagtaatcca gcaactcttg tgggaaatct ttggcggtta
    11101 aacgcggcat cgttgcactc ctcagttggt gtttttttaa gcaaagcata agcacgtgtt
    11161 tttgcccagt ttttcgtcac tctgtgagcc agactacggg atacgcgctg gcgaatcgct
    11221 aaactagaaa cattgtttcg aaattgaacg gtggaaagga gaggtcatgg tctggttagc
    11281 gaatcccgaa cgttacgggc agatgcaata ccgctattgc ggaaaaagcg gtttacgcct
    11341 gcccgcgtta tcgctcggtt tatggcacaa tttcggtcac gttaacgcgc tggaatcaca
    11401 gcgtgcgatc ctgcgtaaag cgtttgattt gggcattacg cactttgatt tagccaacaa
    11461 ttacgggccg cctccaggaa gcgcagaaga gaactttggt cgcctgctgc gggaggattt
    11521 tgccgcttat cgcgatgaac tgattatctc taccaaggct ggctacgata tgtggcccgg
    11581 cccttacggc tctggcggtt cacgtaaata cctgctcgcc agcctcgacc aaagcctgaa
    11641 gcgtatgggg cttgagtatg tcgatatctt ttactctcat cgcgtcgatg aaaatacgcc
    11701 gatggaagaa accgcctctg cgctggctca tgcggtacaa agcggtaagg cgctgtatgt
    11761 cgggatatcc tcttactcgc cagagcggac gcaaaaaatg gtcgagttgc tgcacgagtg
    11821 gaaaattccg ctgttaattc atcaaccttc gtacaattta ctaaaccgct gggtggataa
    11881 aagcggcctg ctggataccc tgcaaaataa cggcgtgggc tgcattgcct ttactcctct
    11941 ggctcaggga ttgctgaccg gaaaatatct caacggcatt cctgaagatt cacggatgca
    12001 tcgtgaaggg aataaagttc gtggcctgtc gccgaaaatg ctcaccgaag ccaacctcaa
    12061 cagcctgcgc ttattgaatg aaatggcaca gcagcgtgga caatcaatgg cgcaaatggc
    12121 gttaagctgg ttgctgaaag atgatcgcgt gacgtcggta ttgattggtg ccagccgcgc
    12181 ggagcaactt gaggagaacg tgcaggcgct gaataatctg acgtttagca ccgaggagct
    12241 ggcgcagatt gatcagcata tcgccgatgg cgagctgaat ctgtggcagg cgtcttccga
    12301 taaatgacct gttaataacg ggcgcgagaa ccgcgcccga atcagatcag tgattatgac
    12361 gagtcagtcg gtcaagatag cccatcacaa atgcagagag cacaaacgtc agatggataa
    12421 tgacgtacca catcagtttg ttatcaggga catttttcgc atccataaag acgcgcagta
    12481 agtgaatgga agaaattgcc acaatcgacg ctgctacttt atttttcagc gacgttgcat
    12541 ccattttccc cagccagttc agcttctctt tgttctcgga gatatcgagt tgtgagacga
    12601 aattctcata accggaaaac atcaccatca ccagcaaacc gccaaccagc gtcatatcca
    12661 ccagcgacag caacacaagg atcaaatctg attccgccat cgagaagata ttcggcagta
    12721 cgtgaataat ctcctggaag aacttcagcg ccagggcaac taacgccagc gaaaggccaa
    12781 agtacacggg ggcaagcagc cagcgagaag catacattgc attttcaaga aaacgttcca
    12841 tacattcctg tcgtgtagtt aaaacgggca acagtatatc gcaattgagt aaacagcgcg
    12901 caacagcaaa tcaacatatc cgttaaacct ttgtcgcata attctgaggg ttagggatct
    12961 tcctggttcc cttatcaacg catcgagagc gtctaccctt aaaggacgtt gaacctgaag
    13021 ggagaaaaac gatgtctcat ttaaaagacc caaccacgca gtattacact ggtgaatatc
    13081 ccaaacagaa acaaccgacg ccaggcatcc aggcgaagat gacaccggta ccggattgcg
    13141 gcgagaaaac ctatgttggt agcggtcgcc tgaaagatcg taaagccctg gtgacagggg
    13201 gcgattccgg aatcggtcgc gctgccgcca tcgcttacgc gcgtgaaggg gctgacgtgg
    13261 cgatcagtta tcttcccgtg gaagaagaag acgctcagga tgtgaaaaag atcattgaag
    13321 aatgcggacg caaagccgtt ctgctgccag gcgatttaag cgatgagaaa tttgcccgtt
    13381 cgctagttca cgaagcgcat aaggccttag gcgggctgga tattatggcg ctggtcgccg
    13441 ggaaacaggt tgccattccg gatattgcag acctcaccag cgaacagttt caaaagacct
    13501 ttgccattaa cgttttcgcg ctgttctggc taacccagga agcgatcccc atgctaccga
    13561 aaggtgcaag tattatcacc acttcgtcaa tccaggcata ccagccaagt ccgcatttac
    13621 tggactatgc ggctacgaag gcggcgattc tgaactacag ccgaggcctg gcaaaacagg
    13681 tcgcggagaa aggtattcgg gtaaatattg tcgcgccagg cccgatctgg acagcactgc
    13741 aaatttccgg cggacaaacg caggataaga tcccgcagtt tggtcagcaa acgccgatga
    13801 aacgtgcggg gcaaccggcg gaactggccc ctgtatatgt ttatctggca agtcaggagt
    13861 cgagctacgt caccgcagaa gtgcacggtg tgtgcggcgg cgagcattta ggttaaaaaa
    13921 atgcccggtt gtgaaaagca accgggcatc attgtgaatt cactcttacc tgatgtggcg
    13981 aaaacgcctt atccgcctac aaacgcatga agattcaaca tgttgcaata gattttgtag
    14041 gcctgataag cgtagcgcat caggcattga gcacctaatg ccggatgcgg cgtgaacgcc
    14101 ttatccggcc tacaaacgca cgaagattca acatgttgca atagattttg taggcctgat
    14161 gagcgtagcg catcaggcaa ttctacttta cttcgctttg gcggtttctt cgccgaccag
    14221 acctatcttc aggtaacccg cctgatgcag cgtatccatt accttcatca acgtctcgta
    14281 atcgacggtt ttatccgctc ggaagaagat ggtggtgtct ttcttgcctt cggttaacgc
    14341 attcaacgcc gtaatcattg tttcatcggt gaccggatcg ttaccgataa acatcgagtt
    14401 gtctgccttc accgacagat aaaccggttt ttccgggcgc ggctgcggcg tgctggtaga
    14461 agcaggcaag ttaaccttca catctaccgt cgctaacggt gccgccacca taaagataat
    14521 cagcagaacc aacatcacgt cgataaacgg cgtcacgttg atatcatgca tttcgccgtt
    14581 atcgtcgagg ttttcgttaa gatgcattgc catcggacat tatcctgcac gtaatttttg
    14641 tgcgacacga accggatgcg cagcggcgct ggcttccaga tccaggtcac ggctttgcag
    14701 caacaatacc tgcgctgcga catcacccag catcgcttta aagccgccaa tctgacgcgc
    14761 gaatacgtta tagataacga ccgcaggaat cgctgccacg aggccgattg ccgttgctaa
    14821 cagagcttct gcgatacccg gcgcaacgac tgccaggtta gtagtctgcg tttgcgcgat
    14881 accaataaag ctgttcatga tgccccagac cgtaccaaac agaccaacga acggcgaaat
    14941 cgcgccgatg gttgccagat agccgttacc gcggcccatt tgacgaccta ctgcggcgac
    15001 ccgacgctcc agacggaagc tggtacgttc tttaatacct tcgttatcgt cgctgccttc
    15061 tgacagttcc agctcattct gcgcttcatt gagcaaatgc aggcttaagc ttttgctacc
    15121 aaaatcagcg gcgatatcgt tggcctggtt taaggaacgc gcttcagcca gcagttgctg
    15181 ctcgcgctta aggcgacgct tctgattgaa gaactctacg ctcttactga agaagattgc
    15241 ccaggtgact acggaggcca aaataagccc aatcatcacg cacttaacga caatatcggc
    15301 gtgctgatac ataccccaga cggaaaggtc cgtctgcatt aaattattac ccactgtgta
    15361 tctccaggac gcaagtcaca aaatctgcgc ataataatat caaaacgacg tcgaattgat
    15421 agtcgttctc attactattt gcatactgcc gtacctttgc tttcttttcc ttgcgtttac
    15481 gcagtaaaaa agtcaccagc acgccatttg cgaaaatttt ctgctttatg ccaattcttc
    15541 aggatgcgcc cgcgaatatt catgctagtt tagacatcca gacgtataaa aacaggaatc
    15601 ccgacatggc ggacaaaaag cttgatactc aactggtgaa tgcaggacgc agcaaaaaat
    15661 acactctcgg cgcggtaaat agcgtgattc agcgcgcttc ttcgctggta tttgagagta
    15721 tggaagccaa aaagcacgcg acgcgcaatc gcgccaatgg cgagttgttc tatggacggc
    15781 gcggaacgtt aacccatttt tccttacaac aagcgatgtg tgaactggaa ggtggcgcag
    15841 gctgcgcgct atttccctgc ggggcggcag cggttgctaa ttcgattctt gcttttgtcg
    15901 aacagggcga tcatgtgttg atgaccaaca ccgcctatga accgagtcag gatttctgta
    15961 gcaaaatcct cagcaaactg ggcgtaacga catcgtggtt tgatccgctg attggtgccg
    16021 atatcgttaa gcatctgcag ccaaacacta aaatcgtgtt tctggaatcg ccaggctcca
    16081 tcaccatgga agtccacgac gttccggcga ttgttgccgc cgtacgcagt gtggtgccgg
    16141 atgccatcat tatgatcgac aacacctggg cagccggtgt gctgtttaag gcgctggatt
    16201 ttggcattga tgtttctatt caagccgcca ccaaatatct ggttgggcat tcagatgcga
    16261 tgattggcac tgccgtgtgc aatgcccgtt gctgggagca gctacgggaa aatgcctatc
    16321 tgatgggcca gatggtcgat gccgataccg cctatataac cagccgtggc ctgcgcacat
    16381 taggtgtgcg tttgcgtcaa catcatgaaa gcagtctgaa agtggctgaa tggctagcag
    16441 aacatccgca agtagcgcga gttaaccacc ctgctctgcc tggcagtaaa ggccacaaat
    16501 tctggaaacg agactttaca ggcagcagcg gactattttc ctttgtgctt aagaaaaaac
    16561 tcaatgatga agagctggcg aactatctgg ataacttcag tttattcagc atggcctact
    16621 cgtggggcgg gtatgaatcg ttgatcctgg caaatcaacc agaacatatc gccgccattc
    16681 gcccacaagg cgagatcgat tttagcggga ccttgattcg cctgcatatt ggtctggaag
    16741 atgtcgacga tctgattgcc gatctggacg ccggttttgc gcgaattgta taacattgcc
    16801 acttttggac aattttgcag acattttatt gtgaaaagtc ttaaattgtt gcgtccggga
    16861 tcaaggcgtc ccggacgatt caggagtaca ataggcagat aaaggcttaa acgctgttcc
    16921 acaggaaagt ccatggctgt tattcaagat atcatcgctg cgctctggca acacgacttt
    16981 gccgcgctgg cggatcctca tattgttagc gttgtttact ttgtcatgtt tgccacgctg
    17041 tttttagaaa acggcctgct gcccgcctca tttttgccag gcgacagctt gttgatactg
    17101 gcaggcgcat tgattgccca gggggttatg gattttctgc ctacgattgc gattctgacc
    17161 gccgcagcaa gtctgggctg ctggctaagt tatattcagg ggcgctggtt agggaatacc
    17221 aaaacggtga aaggctggct ggcacagctt cctgctaaat atcatcagcg cgccacctgc
    17281 atgtttgacc gccacggtct gctggcgctg ctggctggac gttttcttgc atttgtccgt
    17341 acgctgctgc caaccatggc gggaatttcc ggtctgccaa accgccgttt ccagtttttc
    17401 aactggttaa gcggattgct gtgggtcagc gtggtaacca gtttcggcta tgccttaagt
    17461 atgattccgt tcgttaaacg ccatgaagat caggtaatga cgttcctgat gatcctgcca
    17521 attgccttgt taaccgctgg cttgttgggc acgctgtttg tggtgattaa aaaaaaatac
    17581 tgtaacgcct gacgattttc cccgttcccg gttgctgtac cgggaacgta tttaattccc
    17641 ctgcatcgcc cgcattcttg ccgcatcttc ccccggcgtc acaccgaagt aacgtttaaa
    17701 ctcacggcta aattgcgatg cgctttcata gccgacgcgc atcgccgccg cgctggcctt
    17761 catgccgtcg tggatgatca tcatccgcgc cttatgcaga cggtaattct tcaaatactg
    17821 caacggcgag gtgctggtga cagacttaaa attatggtgg aacgccgata cgctcatgtt
    17881 ggcttctgcc gccagttgct cgacgctcag gttttcggtg tatttattct caatccgttt
    17941 cagcacgcgg ctaatcaggc tgaagtgagt ctggcgactg accagcgcca gtaacgcacc
    18001 gccgcaaggt ccggtcagca cgtagtacag aatttcgcgg atgatctgtt tgccgagaat
    18061 acgcgcatcc agtggtcgct ccatcacgtc gagtaaccgc tccgccgcgc ataaaatctc
    18121 ttctgataac gtggcggagt taatcccgct ggctgccatc gacggctgga aatgctcatc
    18181 ttcgccaatg tccatcaaca gttcctgtaa atgcaaaata tcgacgttga gacgcaaccc
    18241 tgccagcggc acctctgacg tggcataggt ttcgcactca aacggcaacg gcaccgtcag
    18301 cagcaggtat tcattggcat cataacgaaa cacgcgttca ttgatataac cgattttatg
    18361 cccggaaaag agaattatga tgccaggctc gtacatcacc ggtgtacgtg cgaaaggcgt
    18421 ctcgccatac aacaaacgca catcgggcaa cagttctgac aaactatttt ctttattttt
    18481 cagtttatta actttatccg ccagcaagcg gcaaatctct tcacgtttca tatcgcgtaa
    18541 tttcttagga ataatgcggc aatttgattg tgcgcaattt tgtagcattt ctccagcact
    18601 ctggagaaat aggcaagaca ttggcagaaa tgagcattga gagccagggc gctggcgatc
    18661 acaatgaaaa acatcaggca gatcgttctc tgccctcata ttggcccagc aaagggagca
    18721 agtaatgaac aactttaatc tgcacacccc aacccgcatt ctgtttggta aaggcgcaat
    18781 cgctggttta cgcgaacaaa ttcctcacga tgctcgcgta ttgattacct acggcggcgg
    18841 cagcgtgaaa aaaaccggcg ttctcgatca agttctggat gccctgaaag gcatggacgt
    18901 gctggaattt ggcggtattg agccaaaccc ggcttatgaa acgctgatga acgccgtgaa
    18961 actggttcgc gaacagaaag tgactttcct gctggcggtt ggcggcggtt ctgtactgga
    19021 cggcaccaaa tttatcgccg cagcggctaa ctatccggaa aatatcgatc cgtggcacat
    19081 tctgcaaacg ggcggtaaag agattaaaag cgccatcccg atgggctgtg tgctgacgct
    19141 gccagcaacc ggttcagaat ccaacgcagg cgcggtgatc tcccgtaaaa ccacaggcga
    19201 caagcaggcg ttccattctg cccatgttca gccggtattt gccgtgctcg atccggttta
    19261 tacctacacc ctgccgccgc gtcaggtggc taacggcgta gtggacgcct ttgtacacac
    19321 cgtggaacag tatgttacca aaccggttga tgccaaaatt caggaccgtt tcgcagaagg
    19381 cattttgctg acgctaatcg aagatggtcc gaaagccctg aaagagccag aaaactacga
    19441 tgtgcgcgcc aacgtcatgt gggcggcgac tcaggcgctg aacggtttga ttggcgctgg
    19501 cgtaccgcag gactgggcaa cgcatatgct gggccacgaa ctgactgcga tgcacggtct
    19561 ggatcacgcg caaacactgg ctatcgtcct gcctgcactg tggaatgaaa aacgcgatac
    19621 caagcgcgct aagctgctgc aatatgctga acgcgtctgg aacatcactg aaggttccga
    19681 cgatgagcgt attgacgccg cgattgccgc aacccgcaat ttctttgagc aattaggcgt
    19741 gccgacccac ctctccgact acggtctgga cggcagctcc atcccggctt tgctgaaaaa
    19801 actggaagag cacggcatga cccaactggg cgaaaatcat gacattacgc tggatgtcag
    19861 ccgccgtata tacgaagccg cccgctaagc tttttacgcc tcaaactttc gttttcgggc
    19921 atttcgtcca gacttaagtt cacaacacct caccggagcc tgctccggtg agttcatata
    19981 aaggaggaac gtatggctaa tccaaccgtt attaagctac aggatggcaa tgtcatgccc
    20041 cagttgggac tgggcgtctg gcaagcaagt aatgaggaag taatcaccgc cattcaaaaa
    20101 gcgttagaag tgggttatcg ctcgattgat accgccgcgg cctataagaa cgaagaaggt
    20161 gtcggcaaag ccctgaaaaa tgcctcagtc aacagagaag aactgttcat caccactaag
    20221 ctgtggaacg acgaccacaa gcgcccccgc gaagccctgc tcgacagcct gaaaaaactc
    20281 cagcttgatt atatcgacct ctacttaata cactggcccg ttcccgctat cgaccattat
    20341 gtcgaagcat ggaaaggcat gatcgaattg caaaaagagg gattaatcaa aagcatcggc
    20401 gtgtgcaact tccagatcca tcacctgcaa cgcctgattg atgaaactgg cgtgacgcct
    20461 gtgataaacc agatcgaact tcatccgctg atgcaacaac gccagctaca cgcctggaac
    20521 gcgacacaca aaatccagac cgaatcctgg agcccattag cgcaaggagg gaaaggcgtt
    20581 ttcgatcaga aagtcattcg cgatctggca gataaatacg gcaaaacccc ggcgcaaatt
    20641 gttatccgct ggcatctgga tagcggcctg gtggtgatcc cgaaatcggt cacaccttca
    20701 cgtattgccg aaaactttga tgtctgggat ttccgtctcg acaaagacga actcggcgaa
    20761 attgcaaaac tcgatcaggg caagcgtctc ggtcccgatc ctgaccagtt cggcggctaa
    20821 cgtgcaaatt ctcccggtgg cggtaatgtt ccgctaccgg acttttcaga aatcatttat
    20881 tcccctcgcg tcccgcccgt tgttactctt ccttgttcag gaatgccaaa tataaggaca
    20941 tcatcatgca gagccggaag ctcttaaaag aacaactcat ctatatccgg gataaacgca
    21001 acggagaggt gaaaaacaga tgaaaataat acttctgttt ttagcagccc tggcaagttt
    21061 taccgtacac gcacagcccc ccttacagac cgtagaacaa acagtccggc atatttatca
    21121 gaactataaa tcagatgcca ctgcccctta ttttggtgaa accggagagc gggcgataac
    21181 ttctgcgcgt attcaacagg cgcttaccct gaacgacaat cttacgctgc cgggcaatat
    21241 tggctggctg gattatgatc cggtttgtga ttgtcaggat tttggcgatc tggtgctaga
    21301 aagcgtagcg ataactcaaa ctgacgccga tcatgccgat gccgttgtgc gctttcgtat
    21361 ctttaaagat gataaagaaa agaccacgca gacactgaaa atggtggcgg aaaatggtcg
    21421 ttgggtcatt gacgatattg tcagcaatca tggcagcgtc ttacaagcag ttaatagcga
    21481 gaatgaaaaa acgctggccg ctttagcttc gttgcaaaaa gaacagccgg aagcctttgt
    21541 tgccgaactc tttgaacata ttgccgatta tagctggccg tggacgtggg tggtttccga
    21601 ctcttaccgc caggcggtca atgccttcta taaaaccacc ttcaaggcgg ccaacaatcc
    21661 cgatgaagat atgcaaatag aacggcaatt tatttacgac aatccgatct gttttggcga
    21721 agagtcgcta ttttcacgcg ttgatgaaat tcgagtcctg gagaaaaccg ccgattccgc
    21781 ccgcattcat gttcgtttta cgctgaccaa tggcaacaac gaagagcaag agctggtttt
    21841 acagcggcgc gaaggtaagt gggaaatcgc tgattttatc cgcccgaaca gcggcagcct
    21901 acttaagcag attgaggcaa aaaccgccgc cagattaaag caatgagctg aattaaataa
    21961 caattagccg gaacaataaa aaagggaaca ctatatgaaa acgattttca ccgtgggagc
    22021 tgttgttctg gcaacctgct tgctcagtgg ctgcgtcaat gagcaaaagg tcaatcaact
    22081 ggcgagcaat gtacaaacat taaatgtcaa aatcgcccgg cttgagcagg atatgaaagc
    22141 actacgccca caaatctatg ctgccaaatc cgaagctaac agagccaata cgcgtcttga
    22201 tgcacaggac tattttgatt gcctgcgctg cttgcgtatg tacgcagaat gataaaaaaa
    22261 tccccggcag catgtcagtt gccggggatt ttttttaacg tccaaccgcc gctttagggc
    22321 gtttcttcgc accagcattc accggacgag attgcgtaga cgacgctttt tttgccgtag
    22381 caggcgtctg acgctgggtc gccatcggcg tatgtttcgt caacgccgga cgggtattgc
    22441 ggttctggcg acgagcttca cgcatctctt caatggttgg cgcaggcact aagcaatcgc
    22501 gacggctgcc aatcagatgc tttttgccca tcgcttccag cgcctggcgg attaacggcc
    22561 agtttgccgg atcgtggtaa cgcaacaacg ctttatgcaa acgacgctgt ttgtcgccct
    22621 tcggtacgaa gacgtcttcg ctcttataac caatcttcgc cagcgggttt ttcccggtgt
    22681 aatacatggt ggttgagttc gccagcggtg acggatagaa gttctgcacc tggtcgagac
    22741 ggaaacgatg ctttttcagc cacagcgcca gattcaccat atcttcatca cgcgtaccgg
    22801 ggtgcgcgga gatgaaatag gggatcagat actgctcttt acctgcctgt tttgagtaag
    22861 tatcgaacag ctctttaaag cggtcatagc tgcccatgcc cggcttcatc atcttcgata
    22921 acggcccttc ttcggtatgt tccggggcaa tcttcagata accgccgacg tgatgggtcg
    22981 ccaactcttt gatatagcgc ggatcttcca cggcgatgtc ataacgtacg cctgaggcga
    23041 tgaggatttt tttaatgcct ttcagatcac gcgcacggcg atagaggttg atcgtcggtt
    23101 cgtggttagt gtccatgtgc ggacaaatat ccggataaac gcacgacaaa cggcgacaag
    23161 tttgttcagc gcgtggcgat ttgcagcgca acatatacat gttggcagtt ggcccaccga
    23221 gatcggaaat tacgcccgta aaaccaggaa cggtgtcgcg gatcgcttcg atctcattaa
    23281 tgatcgaatc ttccgaacgg ctctgaataa tgcgcccttc gtgctcggtg atagaacaga
    23341 aagagcagcc gccaaagcag ccgcgcataa tgttgaccga gaaacggatc atttcgtaag
    23401 ccggaatacg ggcattgcca taggccggat gcggcacgcg cttgtacggc agcgcaaaaa
    23461 cgctgtccat ctcttcggta gaaagcggga tagcaggcgg gttgatccac acatagcggt
    23521 caccgtgttt ttgcatcaat gcgcgggcgc agcctgggtt ggtttcgtgg tgcagaatgc
    23581 gcgaagcatg agcgtacagg actttatcgc ccttcacttt ctcgaaagaa ggcagcaata
    23641 cgtaggtttt ttcccacggt ttcgggcgcg gcggctgcac ggttacagct ttcgcttcct
    23701 gctttttcgg cgcaacaggt ttgttatccg cgcacggcag atcttcgcca tacggatgcg
    23761 ggattgggtc gattttccct ggggtatcaa gacgagtaga atccacaccg ctccagccag
    23821 gcagcgcctc ttttacgata attgcggtat tacgcacatc gcggatttcg ctaattggct
    23881 cgcccatcgc cagacgatgc gctacttcca ccagcggacg ttcaccgtta ccaaacatca
    23941 gcatgtcggc tttcgaatcc accagcacgg aacggcgcac ggtatcggac cagtaatcat
    24001 aatgcgctgt acggcgcaga ctagcctcaa taccgccaag gatcaccggc acatctttcc
    24061 acgcctcttt acaacgctgg gtataaacca gtgtggcgcg atccggtcgc ttacccgcga
    24121 cgttatccgg tgtgtaggca tcgtcatgac gtaaacggcg atcggcggta taacggttga
    24181 tcatcgaatc catgttgcca gcagtaacac cgaaaaacag attcggttta cccagacgca
    24241 tgaaatcgtc tttgctgctc cagtctggct gggcgatgat cccgacgcga aagccctgtg
    24301 cttccagcat acgaccgcaa atcgccatcc cgaagcttgg gtgatcgaca tacgcgtcgc
    24361 cagtaaccaa aatgatgtcg cagctatccc agccaagttg atccatctct tcacgagaca
    24421 tcggcaaaaa cggtgccggt ccaaaacagg cggcccagta ctgcggccag gagaacaggt
    24481 cgcgatccgg ttggatcagg gagatagagc tcattttgct tccagaaatg ataaaaaaat
    24541 aatcaaaggc cggggattat aagccggaac gaaagagaaa tcgaaaggta ttccatactc
    24601 gccctcctcg ggcgagtatg aagattacgg taccggattg accaaaagtt gccctatcga
    24661 gccacggtcc gccatttcca gcgtctgact gttgaagtag aacgggaagt gcgcccagga
    24721 aggctgaccg aaataaacaa gcagctccac ttgtccgtca acccaaacgg tatctttcca
    24781 gcctctgtct tccgggaacg gcatcgcgcc attcacgtta cggatctgga acattacgcc
    24841 ttcaatatgg aacgcttgcg gctcgtccgc gcgtaccgtc cagcgttccc acgttccttg
    24901 ctgcgcggtg acatcaatac ggttgacgtc ccacagctgt ccattaatac ccgggtcatc
    24961 acccagactg atatcgcgac tgcgaattgg cgaaccagcc atgatttcag ttggcagcaa
    25021 gcgcatcgga agactgtctg tgaccagcgg cagaaggccg gttgggcgca gcgttagcac
    25081 cagggtagaa accagaatac tggatggctc aaagaagccg cgaatacgat caacaatgct
    25141 cgccgcttcg ccacaggtga tcgacacttc atcgccgttg ctcatatcca ccagaatctc
    25201 gcggcgctcg cccggtgcca gcgaaagttg cttcaccgat acaggagcag gcaggaatcc
    25261 ctgatcgcca gaaatcacat gtaacgggcg accatcgctc atctgtagtt gatagcgacg
    25321 agagttcgac gcgttcagca gtcgcaagcg cacccagcca cgcgagactt caacgtacgg
    25381 gctttgtaca ccgttaacca gcagcgtatc accaacaaag ccgccgcttc ccggttcgtt
    25441 gtattctggc gtaccaaagt tatccagccg tttatcctgg ataatgaccg gaaaatcatc
    25501 cacaccataa tggttgggga taggcagcga cttgctgact tcatcttcca ccagccacat
    25561 tccggcaagg ccgttataga cctgctgagc cgtgcggttg ggagtattgg cgtgatacca
    25621 cagagtagct gcgttctggc gaatgggcag tacgggtgcc cagtcagcgt ttggcgacat
    25681 catccgtgcc ggaccgccca tcagcgggcc tggtacctgt agcccggcca ccgtcattga
    25741 gacattttct gtcaggcggt tgctgtaaat aagcttaaca tcgtcgccct tccagacgcg
    25801 gatagtcggc cccaggtaac gaccattgat tccccagacc gacgcgcgtg tccctggcgt
    25861 aaatgaccag tgcgcacgtt gtacagtcat aaacagcggt tgcccacggc gagattcaag
    25921 tagcggcgga acgggtagcg gttgctgttg cccggctgcg ctggccttca ggggaacagc
    25981 gcctgcacaa agtgcaatcc ccgatgcctg aatgaactga cgccgactga gtgacatatt
    26041 tgctccatgt aaaactggct aaagtaatac cgtgcaggaa agtgttcccc gcaaaaatac
    26101 agattgccga cttaaacttt tccggcggct tcgcgttctg cgacttcttt atcgagctcg
    26161 gcgatttttt gttccattat cgaacgacaa tgggcagcca gctcacgaac ctgatctttg
    26221 ccatactgac tgacgtcaat tggcggcagc atttcgacaa tcaccagacc gttgtgcagt
    26281 cgattaagat taatcttatt cgaagttgta gagacgcaca cgggaataat cgggacgccc
    26341 gccgcaattg ccgcgtgaaa tgctccagtc ttgaacggta gcaggccgcg accacggctg
    26401 cgggttcctt ccgggaacat ccagatggaa atacggcgtt ttttgaaatg attcacgact
    26461 tccgcaatgg tgccgtgagc tttagtgcga ttgtttctgt cgatcaataa gttgccggtt
    26521 aaccagtaca actgcccgaa gaaggggatc cacagcaagc tctttttacc taccgtcacc
    26581 gtcggcggtt gcacaatgtt cgatgctgtc accatgtcat agttgttctg gtggttagcg
    26641 atatagatag cattgccgta gctttcagca tctgcaggtt tgcggcactc aactttcagg
    26701 ccgaacagcg gcgcaagacg gccaaacata tgcccaaagg tggccacatg tttcgggtta
    26761 cgcgggctga aaaggcagta aatggagccg aatacacaga ctaagatgct gtaaatcacg
    26821 gtaataataa gacgaaagat atatagcata acaccctctg aaggtctgag agactggcat
    26881 tgtacgtcac ctgaaatcag gttagggcat tgttaaaagc cgctctctgg aaaagcgtat
    26941 tgttaatcgc aacgcatgaa taaacaacgg tttcaaggga atttttatcg tttccggggg
    27001 ttgaatacgc tgccggatga cgacttaacg tttcatccgg cgttccttgc aagcgggagg
    27061 aaacagcgcc ctccccggca tattactctt cgctatcacc gctgctggca cggcgaggag
    27121 agtcgatctc aacacgatcg atacgctgca aaccgcgcat caacgtaccg cggcgtccac
    27181 gttcgccagt gactttctgt agctcttccg gacgcagttt aattttgcgt ttcccaacat
    27241 gaatggtcag cgtgctttgc ggcggcagaa cgtacagttg cgccagacca tcctcgccac
    27301 gcgcggcttc tgccgatgga atgttgataa tcttgttgcc tttgcccttc gacagctgcg
    27361 gcagatcgct taccgggaac atcaacatac ggcctgcctg agtgattgcc agcagcatgt
    27421 cggaagcatc ttcaatcacc accggcggca taacatgggc attttccggt aaggttatca
    27481 aagctttacc tgcacggtta cgcgccacca gatcgttaaa ggtgcagacg aaaccgtaac
    27541 ccgcatcgga agccatcagc agtttctgat cgtcgctttc catcagcata tggtcaacgg
    27601 tcgccccagg cggcaacgtt aatttgccgg tgagcggctc gccctgacca cgcgccgacg
    27661 gcagcgtaat cgggtcgatg gcatagctac gaccggtgga atcaacaaac actaccggtt
    27721 ggttgctctt acctttcacc gcagctttga agctatcacc cgctttataa ttcaggcccg
    27781 gcgcgtcgat atcatggcct ttagcgctgc gtacccagcc catctgcgac agcacaatgg
    27841 tgacaggttc agacggcagc atgtcgtgct cgctcatcgc tttcgcttct tcgcgttcct
    27901 gcaacggcga acgacgatcg tcaccgtagg cttgcgcgtc tgcctgcagt tctttcttca
    27961 gcaggttatt cattttacgc tcggaagcca aaatgccctg caactggtcg cgctcttttt
    28021 ccagctcact ctgctcaccg cgaatcttca tctcttccag tttggcaaga tgacgcagtt
    28081 tcagttcgag gatcgcttcc gcctgggttt ccgtaaggcc aaaccgcgac atcagcgccg
    28141 gtttcggttc atcttcgtta cgaatgatct caatcacttc gtcgatgttg agaaacgcca
    28201 ccagcaaacc ttccaggata tgcaggcgct tgaggacttt ctccagacga tagttcagtc
    28261 ggcggcgcac ggtatcgcga cggaacacca gccattcgga gaggatttcc agcaggtttt
    28321 tcaccgccgg acgaccatcc agaccgatca tattgaggtt aatgcgatag ctcttttcca
    28381 gatcggtggt agcgaagagg tggttcatca cctgatccat atccacgcgg ttggaacgcg
    28441 gcacaatcac cagacgggtc gggttctcgt ggtcagattc atcgcgcaga tcgtcaacca
    28501 tcggcagctt tttgttgcgc atttgcgcag caatttgctc cagtacgcgc gcacctgaaa
    28561 cctgatgcgg taatgcgctg ataaccaccg cgccatcttc tttcttccac accgcgcgca
    28621 tacgcactga accacgtccg ttctcgtaga ttttacggat ctcggcgcgc gaagtgataa
    28681 tttccgcttc agtcggataa tccggcccct gcacgatatc cagcagctga tcgagcgtgg
    28741 ttttcggctg gtcgattaat gcgattgccg cctgagctac ttcacgcagg ttatgcggtg
    28801 gaatatcggt cgccatgccg acggcaatac cggtggtgcc gttaagcaaa atgttaggca
    28861 gacgggcagg tagcattttc ggctcctgca aagtgccgtc gaagtttggt acccagtcag
    28921 ccgtcccctg ccccagctcg ctcaatagca gctcggaata tttcgacaac cgggattcgg
    28981 tgtaacgcat tgccgcgaac gatttcggat cgtccggcgc gccccagttc ccctgaccat
    29041 caaccagcgg ataacggtaa gagaacggct gcgccatcag gaccatcgct tcataacagg
    29101 cgctatcgcc gtgcggatgg tatttaccca gtacgtcacc gacagtacgg gccgattttt
    29161 taaatttggc gctggcattc aggcccagtt cagacatcgc atacacaatg cggcgctgaa
    29221 caggtttcag accatcacca ataaacggca acgcacggtc catgatcacg tacatggagt
    29281 agtttaagta ggcgttttcc gtaaattcat gtagcgcaag gcgctctgcc atatcgctca
    29341 ttaattctga ttcctcaact tattcgccag ccttcaaagg ggcaatattg ccggagatac
    29401 tacattatct gccgcgtcga gtcacaggag atcagcgtga atagcgccgc cggacgtgct
    29461 ccgacggcga taaaggcatc aatgtgcctt gatatacaac tctttgctgt agacttgatc
    29521 ggcatagagg gcagcacaag gttgcgccca tgccacacca ctcggcatgg gggtccgctc
    29581 cgggcgattc gagaagttga ggttatgcga gtccagggaa gcctaaagca tcaaagtagt
    29641 cgactgtaag tatacggttc agcccagttt agctgtgtct ccaccagtgc cccgcgcctc
    29701 ctgccataag tgcggcttgc tcatggatac ccccgagacg gcttacggcg caccggataa
    29761 taaatttcgc gtatacatgg tattccttac cggagaaatt ataagcccaa caacaatacg
    29821 cctgtggcat tgttcgctca aacttatcgc gagtgatttg gctcacactc cggtaaagat
    29881 tccccatcac gccaacggca cataaacatc cgtttccagt cgctgctctg tcaccccttc
    29941 ggcaagattg gtgtagtgaa acagaatcgg tgctttacgc attttctcgc cgcttgcagg
    30001 cagccagtgg cgaataatgc cccatatcgt gtgtgaaata tcgtccagct cgccaacgtg
    30061 gcgggccacg gcataacgtc caccggtaag ttcaccattg ctcacaccat aacgattatc
    30121 gggaatcggt tcgctaacgc tgccgcagat atcaaagcga aacgcttccg gtgcggtggt
    30181 ggcagggtcg tcccaggcga cgccaaaagt ctggctttgg ttaacaggag aaagccccgt
    30241 ctctttacgc cacatgataa atttcgccac actgtagttg agcaactcag ggctacagcg
    30301 gtgcggcaac atcgccacag ggattgatgg gaaatcgata atgtttacat ccagtgtcag
    30361 gtttgtcatt ttgcctccct gcgaaattat tcccgggaac gcgtcccggg agcggtaaca
    30421 attacggatt tactttgcgg atctgtttga cgtcaatttc aacagaattc cagtctttat
    30481 cgacttcacc ctgaatctca accgtatctt tcggcgtcac cgtcacaccg ttccagcgtt
    30541 tgtggtcgat atcaacattg atagtaccgc tggcatcttt aaacacgtag agatcgtcag
    30601 agatgcgttc aacgatattg ccgcgcaggg ttacccaggt gtcgtcacgc agggattttg
    30661 cgctttctac ggtcgttacg ctgccgttcg gcccctggaa tcctccggcc tgactttgcg
    30721 ttgccgatgg gccagaaaaa ccgccctgct ctgctgccat caccggtgcg ctgcacaggg
    30781 ccattactgc gattactgct gcgaattttt tcatgtttat tactcccttt aatgtctgtt
    30841 tccgagcatt taacaagata gtccttaaca acttcttaag ggaaaaaaat aaaatttagt
    30901 gctgtacaga gcgcgttaca acacggttta ctggcagcaa atacggttat cgcagggatg
    30961 aaaaaatgcg aattttactg atagaagatg acatgctgat tggcgacggc atcaaaacgg
    31021 gccttagtaa aatgggtttt agcgtcgact ggtttacaca aggtcgtcag ggaaaagagg
    31081 cgctttatag cgcaccttat gatgcggtga tcctggattt aaccttacca ggcatggatg
    31141 gtcgcgatat tttgcgcgaa tggcgagaaa aaggtcagtg tgagccggta ctgatcctga
    31201 ccgcgcgcga tgcgctggag gaacgtgtag aagggctgcg tctgggagct gacgattatc
    31261 tgtgtaaacc ttttgcgttg atagaagtcg ccgccaggct ggaagctctg atgcgccgaa
    31321 ccaacggcca ggccagcaac gagctgcgcc acggtaacgt catgctcgac cccggcaaac
    31381 gtatcgccac gctggctggc gaacccttaa cactgaaacc aaaagaattt gccctgctgg
    31441 aattactgat gcgtaacgct ggtcgggtac tgccgcgcaa actgattgaa gagaaactgt
    31501 atacctggga cgaagaggtc accagtaatg ccgttgaagt gcatgtgcat catctgcgac
    31561 gcaaactcgg tagtgatttt attcgtaccg tgcatggtat tggctacaca ttaggtgaga
    31621 aatgaaattt acccaacgtc ttagtctgcg cgtcaggctg acgctaatct ttttaattct
    31681 ggcctcggtg acctggctgc tttccagctt tgtcgcctgg aaacaaacaa cggataacgt
    31741 cgatgaattg ttcgacaccc aactgatgct gtttgccaag cggttaagta cgctcgatct
    31801 caacgaaatc aacgcggcgg atcgcatggc acagacgcca aataaattaa aacacggtca
    31861 tgttgatgac gatgcgctga cctttgccat ctttacccac gacggcagaa tggtccttaa
    31921 cgatggcgat aacggagaag atattcctta tagctatcaa cgggaaggtt ttgctgacgg
    31981 gcaactggtg ggcgataaag atcaatggcg ttttgtctgg atgacctcac ctgatggcaa
    32041 atatcgcatc gttgttggtc aggaatggga ataccgtgaa gacatggcgc tggcgattgt
    32101 tgccgggcaa ttgatcccgt ggctggtcgc actgccgatt atgttaatca tcatgatggt
    32161 actattgggt cgtgaactcg cgccgctgaa caaactggcg ctggcgctgc gtatgcgtga
    32221 ccctgactcg gaaaaaccac taaacgcgac tggcgtaccc agcgaagtgc gtccgttggt
    32281 tgagtcgcta aatcaactgt tcgcccgcac acatgcgatg atggttcgtg aacgacgctt
    32341 tacctccgac gcagctcacg aactacgtag cccgttaacg gcgctgaaag tgcaaaccga
    32401 agttgcgcag ctttctgacg atgatccgca agcgcggaaa aaagcactgc ttcaattaca
    32461 ttacgggatc gatcgcgcta ctcgtctggt tgatcaactg ctcactctat cgcggctgga
    32521 ctcactggat aaccttcagg acgtcgcgga gatcccgctt gaagatctcc tgcaatcgtc
    32581 ggtgatggat atttaccaca cggcgcagca ggcgaaaatt gacgtgcgac tgacactcaa
    32641 tgcccacggc atcaaacgca ccggacaacc gctattgcta agtttgttgg tgcgaaattt
    32701 gctggataac gccgtgcgct acagtccaca gggcagcgtg gtagacgtca cgctgaatgc
    32761 tgataatttc atcgtgaggg ataacggccc cggtgtgaca ccagaggcac tggcgcgaat
    32821 tggcgaacgc ttctatcgcc cacccggaca aaccgctacc ggcagtggac ttgggctatc
    32881 gattgtccag cgaatcgcca agctgcatgg catgaatgtt gagtttggga atgcggaaca
    32941 aggtggattt gaggcgatgg taagctggta acttctctgt tctgtctatg agagccgtta
    33001 aaacggctct catagatttt actaatagca aaatataaac cgtccccaaa aaagccacca
    33061 accacaaacc agacaaataa cgaacgagca tacatataac caaaactcaa acatttcccg
    33121 cacaaagagt tttcttaaga taataataag tggcgtcaga agaaaaaatg ctgcaatcat
    33181 tgcttgcaat atacgatctt cgcctgaaac ggaaggtata ctgcaaatat tgtctcccga
    33241 gtcaagcatc cagtcatact tattgaaagc aaaaaaagca tctattaacc aataagtaag
    33301 cattatatat aacagagctt caaatattgt ttgaatttta tgctttaaca tatgttagca
    33361 ctctctaaaa aagtccagtc ggagatctgg ttttcgatac cctccagtaa atctatttaa
    33421 aataatctgc gtaacacgct gatattttca tttttagtta ttcaacacca caattatcaa
    33481 ctcgcagaaa gtccacacca acgttgtaaa ttaaccctgt gctagttgga atgatgagaa
    33541 attgctatca gtattgtcat tttcagaaaa tatttatcct gcatcggtga gtcagagcaa
    33601 gatcagactt ttgctaaatt cgcaaaagac tttgcacatt ttgctaattt caccgtaccg
    33661 ctctgtgacg tactatagtc ggcaaacgtc tcaccttgag gttaaaaaat gagcaacatc
    33721 ctgattatca acggcgcgaa aaaattcgcc cactccaatg gtcaactgaa cgacaccctg
    33781 accgaagtcg cggatggcac actgcgcgac ctcgggcatg atgttcgcat cgttcgcgcc
    33841 gacagcgact acgatgtcaa agcggaagta caaaactttc tctgggctga tgtggtgatc
    33901 tggcagatgc caggctggtg gatgggcgcg ccgtggacag tgaaaaaata cattgatgat
    33961 gtattcaccg aaggtcacgg gactctgtat gccagcgatg gccgtacccg caaagatccg
    34021 tcaaaaaaat acggttccgg cggcctggta cagggcaaaa aatatatgct ttctctgacc
    34081 tggaacgcac caatggaagc cttcaccgaa aaagatcagt tcttccacgg cgttggtgtt
    34141 gacggtgtgt atctgccgtt ccataaagca aaccaattcc tcggtatgga accgctgccg
    34201 acatttatcg ctaatgacgt gataaaaatg cctgatgttc cccgctatac tgaagaatat
    34261 cgcaagcatc ttgtggaaat ttttggttaa ctagagctca ggctttagaa ggagttaacc
    34321 atgcttaccg taatcgcaga aatccgtact cgtcctggtc aacatcaccg tcaggcggta
    34381 ttggatcagt ttgctaaaat cgttccaacc gtactgaaag aagaaggttg ccacggctat
    34441 gcgccaatgg tggattgcgc tgctggcgtg agtttccagt ctatggcacc ggactctatc
    34501 gtgatgattg agcagtggga aagcatcgcg caccttgaag cgcatctgca aaccccgcac
    34561 atgaaggcgt acagcgaagc cgtaaaaggc gacgtgctgg agatgaatat ccgtattctg
    34621 caaccgggga tttaacattc ctgaaaagag gccggtgttg ctaccggcct cagttcagat
    34681 tagccattct ttcttcccgt tttcagcccc cgatggaaat cgttaattcg cttaatggtt
    34741 ttaaccgcac gctgcggcac cgcggaagcc accgaccaga caatcatttc caggcacaac
    34801 aataccgtac catgtagtgg cgttttcccc ttttcatccc cgcgcggaac atgaatcacg
    34861 atactggcgt ctttgctaaa acgtgaatcc agcgcgtttg tcagcaaaat aacagggatc
    34921 cccagccgtc tcgcttcacg cagcgttgtc agcccttccc ggtgcgcaga tttttgcgcc
    34981 atcatgatca gaacatcgcc acgttgaagc gcgatcagtt gctcggcaag gccaataccc
    35041 gtacggttta gtgccgtggc aggcaaacct atgcggttga atagtcgtgc ggtatagtcc
    35101 gccagaatac ccgaagcgcc aatgccaaaa attgcgactt gccgtgcctg taccaacaac
    35161 gctaccgctt gcgcaagcgc gtggcggttt tcaggctctg acaggacatt acaggtatac
    35221 aaatgccctt caagaacaaa gtcgattgcg gtgttaacat cgctcgtcaa gttactcacc
    35281 gtcgtagaca tcttttcgct ggaactaagg gctggaccaa gccactgttc cagagtgcgt
    35341 ttcagatcac gcagcccggc aaaccccagg gcctgtatag ccctgataac tgtggcatcg
    35401 gaagtgttca acgtggccgc aatttccatc gccgtttgct cgagtaccac ttcacgatgc
    35461 tcattaatat agctggcgac agctcttaac ccaggcgtta actgactcgc gcgagtgcga
    35521 aaacgctcac cataaatatc aacccgcgtt ttgtctttct gaatcatttg gcctccggta
    35581 aaggtcgccc cgcaatttgt gattgtatct gcgcagccat cagccccaga gatgcaaagg
    35641 agttagaaat tgcctcttca cgagaaatca gcacataacg cccggaacga caggcagtca
    35701 gaaactgaca ccatcccggc atgacttttt ccattgctgc cagctcatcc tgaggtttac
    35761 caccggtgtc accgcgccag gttgcaaaaa caaaatcggc atccagttca ggtaaacgct
    35821 ccgcgctcac atccattcgc ccaccttcag gaatgctctc aatcagcggc ggaaagcgaa
    35881 aaccagcatc gcgtaatacc cgccccagcg aatgatagct atgcatcaca ttaatttttc
    35941 cctgatttgc ctgaatcacc gataccgtta ttttttggct gtccagcgtc gccttcagcg
    36001 cattgatttg tgcctgatag cggcgttcaa gtatcgccag ctgcgactgc gttcctgtca
    36061 gttctgccag cttgcggtag atttctggcg cgcctccttt gagatgatca atgctcaccg
    36121 tgggcgcaat tttttccagt tgttcaatgg gcgtattacg ggtcggttct gtgataatca
    36181 ggtcaggctt tgccgccaca atcgcttcaa tatcaatatc agcggtgcca ataaaagcga
    36241 tcgatgaatt atcaaagtcg acacctgtca ataacgcgcc tgatcgaatg aaatggctac
    36301 cgtcaggccg ggttcgcccg tggctggcga caggaggtac accgagctca atcagaggaa
    36361 tagtgatatc aagatcgtgt aaagagacaa ttctctttgg atgagcagga acagtgactt
    36421 tgcgccctaa atcatcagta aagacctgaa caggttctgt cgcgcgggca aagaatgaca
    36481 acagtatcag cagagaaaaa aataaacgca tacaactccc attaaaaact gttcctgcgc
    36541 tgccagagca gtagcaaaaa aaatggccca ccaatcagtg agataatgat tcctgcaggc
    36601 agttgcaaag gcaaaaaagc caaacgacca atgctatccg ccagaataac taacaacgcc
    36661 cccgataccg cgctccccag cagtaatgtt gtctgcccgc cacgtagaat aaaacgtgat
    36721 atatggggtg ctatcaatcc gacaaaaccg atgttaccaa cacacgaaac gcagacggct
    36781 gtcaggatta ttggtgcggc gacacgtaac aacgccagac gagaactatt cacccctaac
    36841 ccggttgcaa cctgatgacc aagcagagcg atatccgccg tcctggcggt gaacagcaat
    36901 aaaagagcgg ctggcaacat ccaacaggct gaaataccga ctaacatcca gttagctgca
    36961 tgcaggcttc ccgataacca catcaatgca gtttgtacat ctcgaacatc tgcagtggtc
    37021 ataaatacac cgataccggc ggcaaaaaac caggaaacac caataccaat cagaacaaat
    37081 cgcgggcggg aaatatcgcg tgcacaaaaa atcacgatta aagccaccaa caagccacca
    37141 gcaagaccag ccactggacg ccagaacatt cccagcattg gaaactggaa aatcagccac
    37201 agcaccgcca cgctacatcc ttctttgacg ccgattaaac caggatcggc aaggccgtta
    37261 cgcgcaatgg attgcatcgc ggcccccgcc atacctaaca tggctccgca tagcaatgcc
    37321 atgatgactc tcggcaagcg aatatcctgc acaatataac gtgcgtctgt gcttacgttt
    37381 tctggagcaa acaacgctcg tcctacttcc gatgccgaga cagaaaaaga accatgcatc
    37441 agaccgaata tcagtattcc aaccgcaaac agaaacagcc caccaagcaa caccagattt
    37501 ttagggcgta ccagtgtcga aaacttacca acacgtagtg gacgtagccc gacgttcatt
    37561 atccgactca tttgaacatc ctcgtcgcca taatgacaaa aacaggcgca ccaactaacg
    37621 ccgtcatgac accggtcgct aactcatggg gcgtgaaaag cgtccgagca ataatatctg
    37681 ccagtaataa caaaagcgcg ccgacacaag ctgaaagagg gagcagaacg cgcagatccg
    37741 ctgataccag acgccggaca atctgcggta ctagcaggcc gacaaaacca atggggccag
    37801 caatcgatac ggctgcgcca cagagcaacg cgatcgcaag cagggtgaat gttcgcgtgc
    37861 ggagtaccga gacgcccagc ccctgcgcca tgcgatcacc cagtgccaac atgttaagag
    37921 agggcgcaag gtaaatggct aacacaaatc cccccagaga aaaccaggcg gacgtaccca
    37981 gcgtcaccca atcctgcccc gctaaatcac ctgctagcca tgtccgcatc gccagcagtg
    38041 tttgttcgtc aagaatcagg attgcagccg ttattgagga cacgaacgcc gatagcgcca
    38101 cgccgcataa agtaactttc attggcgtta accctgaacg ccctgcggag gaaagcagca
    38161 atatcaacag gaacaataac gcgccccccg tagatgccag cagtgggcgt ccagccggaa
    38221 aggccagtcc aagcgcactg gcggcaacga cagcaagagc ggctccggca tttaagccta
    38281 aaatatgggg ctcccccaat gggttacgaa tgacagcctg caacaaagca ccggcaacac
    38341 caagagaagc accggtaagc aatgctgccg ctaaacgcgg caaacggagt cttacaatta
    38401 cgttgtgatc gaagttttgc ggatcaaaat gaagaaatgc ctgcaacacc gtttgtggtg
    38461 caataaatcg ggcaccaatc cctggatata ccacgcatcc caccagcagt aaggtgagga
    38521 aaattacagt aaccagccct gtgcgcatca tactttttct acaccacgaa acggcatgaa
    38581 gaacggcttt cccgtcagag gatttattga tgcgtgaaca tcaacgtcaa agacagcttt
    38641 taccagctca ggcgtgcaat gctccacctc attcaacaca cgcaccactt tcccctggcg
    38701 taaaaatatc aacgtatcac cataattgac ggcaaaattc agatcatgca acaccacgac
    38761 gactgtacgc ccgtgatgac gcgttaaatc atgtaataac tcaagaattt caacctgata
    38821 acgtaaatca agccaggttg tgggttcatc cagtaggata taaggcgtct tttgcgccaa
    38881 caccatcgca atccagcaac gctggcgctg cccaccagag agtttttcaa caggtaaatg
    38941 agcaaactcc tgcgtaccgg ttaatttcag ggcctcttct accgcctgct cgtcggcgtc
    39001 gctccattga cgtatgaaat tttgccaggg aaaacgccca cgcgacacca gttcataaac
    39061 ggttaagccc tccggcaata atggcgactg cggcaagatc ccaagtctac gggataacgc
    39121 tttcgttggt tgctcgtgaa tcgctttacc atccagcaat attgagccgc ccatgggttg
    39181 caagatacgt gcaatggtgc tgagcaacgt cgactttcca cagccgtttg ccccaaccag
    39241 aacggtcatt ttttctgtgg gaacggagaa agaaacacca tcaacaatga ttttcttgtg
    39301 atacccggca gaaacattgt cgaggatcag cccctgccct tttgcgttat gtgccacgtg
    39361 tacgccaact taatcaaaga caaacaaaaa taaaactcat tctcctttga gagtttgacg
    39421 ccacaaaatg tagtagtcaa gaagtta
//
LOCUS       DAPGOF010000021        39130 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.021, whole genome shotgun sequence.
ACCESSION   DAPGOF010000021 DAPGOF010000000
VERSION     DAPGOF010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.021"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..391)
                     /locus_tag="RHV13_000976"
     CDS             complement(<1..391)
                     /locus_tag="RHV13_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDV1240531.1"
                     /translation="MKSILIEKPNQLAIVEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGDGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CMTLAVLGVHADGGFSEYAVVPAKNAWK"
     gene            complement(403..1617)
                     /gene="rspA"
                     /locus_tag="RHV13_000977"
     CDS             complement(403..1617)
                     /gene="rspA"
                     /locus_tag="RHV13_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HDV1240532.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCSQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHTGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(1823..2149)
                     /locus_tag="RHV13_000978"
     CDS             complement(1823..2149)
                     /locus_tag="RHV13_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HDV1240533.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWMGALIALCGMLIIV
                     AGWGRT"
     gene            2284..2625
                     /locus_tag="RHV13_000979"
     CDS             2284..2625
                     /locus_tag="RHV13_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HDV1240534.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2660..3220
                     /gene="speG"
                     /locus_tag="RHV13_000980"
     CDS             2660..3220
                     /gene="speG"
                     /locus_tag="RHV13_000980"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HDV1240535.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(3223..3933)
                     /locus_tag="RHV13_000981"
     CDS             complement(3223..3933)
                     /locus_tag="RHV13_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HDV1240536.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            4041..4346
                     /locus_tag="RHV13_000982"
     CDS             4041..4346
                     /locus_tag="RHV13_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HDV1240537.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4545..6971
                     /locus_tag="RHV13_000983"
     CDS             4545..6971
                     /locus_tag="RHV13_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HDV1240538.1"
                     /translation="MSKNDRMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQAREKVVWGACSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDENTLPADAPKNGHYKAYILGE
                     GDDKTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     RTFPLQLFGFHYKSRTHSTYGNIDLLKAACRQEVWINPIDAQKRGIANGDMVRVFNNR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            7032..9455
                     /locus_tag="RHV13_000984"
     CDS             7032..9455
                     /locus_tag="RHV13_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HDV1240539.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEVIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            9466..10083
                     /gene="dmsB"
                     /locus_tag="RHV13_000985"
     CDS             9466..10083
                     /gene="dmsB"
                     /locus_tag="RHV13_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HDV1240540.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            10085..10939
                     /locus_tag="RHV13_000986"
     CDS             10085..10939
                     /locus_tag="RHV13_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HDV1240541.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFTIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            10982..11596
                     /gene="dmsD"
                     /locus_tag="RHV13_000987"
     CDS             10982..11596
                     /gene="dmsD"
                     /locus_tag="RHV13_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HDV1240542.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTRDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            11754..13046
                     /gene="clcB"
                     /locus_tag="RHV13_000988"
     CDS             11754..13046
                     /gene="clcB"
                     /locus_tag="RHV13_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HDV1240543.1"
                     /translation="MHHLHTYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDVQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRESIYRQHTAQHS"
     gene            complement(12999..13694)
                     /gene="bioD"
                     /locus_tag="RHV13_000989"
     CDS             complement(12999..13694)
                     /gene="bioD"
                     /locus_tag="RHV13_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HDV1240544.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRLTV"
     gene            complement(13819..15039)
                     /gene="mlc"
                     /locus_tag="RHV13_000990"
                     /gene_synonym="dgsA"
     CDS             complement(13819..15039)
                     /gene="mlc"
                     /locus_tag="RHV13_000990"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HDV1240545.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(15174..16067)
                     /locus_tag="RHV13_000991"
     CDS             complement(15174..16067)
                     /locus_tag="RHV13_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV1240546.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            16174..17427
                     /locus_tag="RHV13_000992"
     CDS             16174..17427
                     /locus_tag="RHV13_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1240547.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKSLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            17824..18159
                     /locus_tag="RHV13_000993"
     CDS             17824..18159
                     /locus_tag="RHV13_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HDV1240548.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNAKTEQKAPEQKAQAAKKHAKKHSHQQP
                     AKPAAQPAA"
     gene            18252..18335
                     /locus_tag="RHV13_000994"
     CDS             18252..18335
                     /locus_tag="RHV13_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1240549.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            18435..19256
                     /locus_tag="RHV13_000995"
     CDS             18435..19256
                     /locus_tag="RHV13_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HDV1240550.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYDIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LSLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(19295..19624)
                     /gene="mdtI"
                     /locus_tag="RHV13_000996"
     CDS             complement(19295..19624)
                     /gene="mdtI"
                     /locus_tag="RHV13_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HDV1240551.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(19611..19976)
                     /gene="mdtJ"
                     /locus_tag="RHV13_000997"
     CDS             complement(19611..19976)
                     /gene="mdtJ"
                     /locus_tag="RHV13_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HDV1240552.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            20388..21422
                     /gene="tqsA"
                     /locus_tag="RHV13_000998"
     CDS             20388..21422
                     /gene="tqsA"
                     /locus_tag="RHV13_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HDV1240553.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(21447..22835)
                     /gene="pntB"
                     /locus_tag="RHV13_000999"
     CDS             complement(21447..22835)
                     /gene="pntB"
                     /locus_tag="RHV13_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HDV1240554.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(22846..24378)
                     /gene="pntA"
                     /locus_tag="RHV13_001000"
     CDS             complement(22846..24378)
                     /gene="pntA"
                     /locus_tag="RHV13_001000"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HDV1240555.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            24902..25846
                     /locus_tag="RHV13_001001"
     CDS             24902..25846
                     /locus_tag="RHV13_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDV1240556.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            26032..27414
                     /locus_tag="RHV13_001002"
     CDS             26032..27414
                     /locus_tag="RHV13_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDV1240557.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            27451..28173
                     /gene="folM"
                     /locus_tag="RHV13_001003"
     CDS             27451..28173
                     /gene="folM"
                     /locus_tag="RHV13_001003"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HDV1240558.1"
                     /translation="MGQTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(28170..28505)
                     /locus_tag="RHV13_001004"
     CDS             complement(28170..28505)
                     /locus_tag="RHV13_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HDV1240559.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            28634..29353
                     /gene="rstA"
                     /locus_tag="RHV13_001005"
     CDS             28634..29353
                     /gene="rstA"
                     /locus_tag="RHV13_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HDV1240560.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            29357..30658
                     /gene="rstB"
                     /locus_tag="RHV13_001006"
     CDS             29357..30658
                     /gene="rstB"
                     /locus_tag="RHV13_001006"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HDV1240561.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            30734..31663
                     /gene="tus"
                     /locus_tag="RHV13_001007"
     CDS             30734..31663
                     /gene="tus"
                     /locus_tag="RHV13_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HDV1240562.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(31660..33063)
                     /gene="fumC"
                     /locus_tag="RHV13_001008"
     CDS             complement(31660..33063)
                     /gene="fumC"
                     /locus_tag="RHV13_001008"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HDV1240563.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLNEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(33206..34852)
                     /gene="fumB"
                     /locus_tag="RHV13_001009"
     CDS             complement(33206..34852)
                     /gene="fumB"
                     /locus_tag="RHV13_001009"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HDV1240564.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            35051..36226
                     /gene="manA"
                     /locus_tag="RHV13_001010"
     CDS             35051..36226
                     /gene="manA"
                     /locus_tag="RHV13_001010"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HDV1240565.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLCKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            36327..37835
                     /locus_tag="RHV13_001011"
     CDS             36327..37835
                     /locus_tag="RHV13_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HDV1240566.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITVAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(37880..39127)
                     /gene="uidC"
                     /locus_tag="RHV13_001012"
     CDS             complement(37880..39127)
                     /gene="uidC"
                     /locus_tag="RHV13_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HDV1240567.1"
                     /translation="MTVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRADKPSAG
                     AGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGDKSFGFA
                     LGAVKIKPSENSLLKIGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALGYWALTP
                     NIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDTSRYSLG
                     TSVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETALVSGQL
                     TWNAPWGSVFGSGGYLRHAINGAVVDTDIGYPFSLSLDRNREGMQSWQLGVNYRLTPQ
                     FTLTFAPIVTRGYESSKRDVRIEGTGILGGMNYRVSEGPLQGMNFFLAADKGREKRDG
                     STLGDRLNYWDVKMSIQYDFMLK"
BASE COUNT         9733 a        10086 c         9521 g         9790 t
ORIGIN      
        1 ttttccacgc attttttgcc ggaaccacgg catattcact gaaaccaccg tcagcgtgca
       61 cacctaatac agccagtgtc atacaaacgt tcggtttacc tatagagcac ggatagcaat
      121 gcccacagct gaccaccgga tcgacagcaa cacgttcacc gactctggcg ctttccacgc
      181 cgtcacccac tgcatcaatg acgccaaaga attcatgacc aatgacgcgc ggatatttcg
      241 caaaaggatt atgcccacga taaatatggc tatctgaacc acaaattccg gcaagtttca
      301 cttttactcg tacttcaccc gctgacgggg tgggtatttc acgttcgaca atcgccagtt
      361 gattcggttt ttcaattaat atgcttttca ttatcttact ccttaccagt tccacagcgt
      421 gccatcttcc agacgtgcga ctggtagata agcaggttca tagggatatt tcgccgccag
      481 cttttcatcg aattcgatac caagacccgg tttgtctccc ggatgcatat agccgttatc
      541 gaaagtccag ttgtgcggga agacttcgag catttgttcg gaataaccca tgtattcctg
      601 gacaccgaaa ttggggaccc acagatcgaa atgcagcgcc gcagccatgc agactggtga
      661 caaatcggaa ggaccgtgtg agccagtacg tacctgatac agcgaagcaa aatcggcaat
      721 ccggcgcata ccggtaattc cgcctgtatg ggtcagcgtg gtgcggatat aatcgataag
      781 ttgctcttca atcagttgct tgcagtccca gatgctgttg aagacttcac ccactgcgat
      841 gggtgtgacg gtatgttggc gaatgagacg gaagcattcc tggttttccg caggcgtcgg
      901 gtcttccatc cagaacatgc gataatcttc aatgctttta ccaaagcgcg ccgcttcaat
      961 aggcgttaag cgatggtgca tgtcatgcag caaatgttca ttaaaaccaa acttgttacg
     1021 taccgcgtca aacaatttcg gcatgaaatc gaggtatttc tccgtcgacc acagctgctc
     1081 ttccggccac tgtcctttgg ttgcgggttc ataagccaga cctttacctt tcgacatgcc
     1141 gtaggtggtt ttcataccag ggattccgca ctgcacgcgg atggctttga atcccagctc
     1201 ctgatgacgg gcataatcat ccagagcttc atcaatactg tgaccggtgg tatggcaata
     1261 aaccatcacc ccttcacgag acgcgccgcc gagtaactgg taaagcggca tgttggcagc
     1321 tttggcttta atatcccaca gcgccatatc aaccgctgaa atggccgaca tcgtaaccgg
     1381 accgcgacgc cagtaagcac ctttatagaa aaactgccag atatcttcga tacggtgcgc
     1441 atcgcgacca ataagctgcg aacaaaggtg atcctgcaaa taagaggcca cggaaagctc
     1501 acgtccattg agggtggcat ccccaaggcc cgtaataccg tcctcagtgg tgatttttaa
     1561 tgtgacgaaa ttacgccccg gacaggtaac aaaaacttca gcctttacga tcttcatgtt
     1621 cgattccttg catcgcttgt cgtgatgcat gaaatctacg caactgagct actaccatac
     1681 aagtatgaag atcaaaaaaa gccggagtga tcacaaaaaa aggcgtatat tttcgccatg
     1741 aatggttgac aaaagatgaa atagagtacc ttttatcagc tgacgcttcc tcgtatctta
     1801 ttgataaaat ggatttatgt tcctacgtgc gcccccagcc cgcaacaatg atcaacatgc
     1861 cgcaaagcgc aatcaacgca cccatccagt cataaagagt cagtttcacg ccatccacaa
     1921 cgcgcagcca catcaacgcc gtgcagacat aaacgccacc ataagccgcg taaacacgcc
     1981 cactcgccgc aggatgcaac gttaacaacc agacaaacag cgccagtgaa atccccgccg
     2041 gaagcaacag ccagatactg gcgtttcgtt ttaaccacaa ccagggcaga aagcatccaa
     2101 taatttcaca cagcgcagta gcaaaaaata gtaacgttgt tttaatcatc tttgtcactt
     2161 attgacatca tgtatagtta tagggccaca taatatcatc aatataaaca ccctcctggt
     2221 acgttttgcg ttcgcagtgg atggtgtaga atcaccttta atcattcata cagggaatga
     2281 attatgaaaa tcactctcag caaacgaatc ggcctgctcg cttttctgct gccttgcgca
     2341 ctggcattga gcacaactgt tcatgccgaa actaacaaac tggtgattga gtctggcgac
     2401 agtgcacaaa gccgccagca cgccgctatg gaaaaagagc aatggaatga cacgcgcaat
     2461 ctgcgtcaga aagtgaataa acgcactgaa aaagagtggg ataaagccga cgccgctttt
     2521 gataaccgcg ataaatgtga gcaaagcgcc aacatcaatg cctactggga gcccaatact
     2581 ttgcgctgcc tggaccgtcg aactggccgc gttattaccc cctaacctgt tattgattta
     2641 aggaatgtaa ggacacgtta tgccaagcgc ccacagtgtt aagctacgcc cgctggagcg
     2701 tgaagattta cgctatgtac atcaactcga caataacgcc agtgtgatgc gttactggtt
     2761 tgaggaaccc tacgaagcct ttgttgaact ctctgatctg tatgataagc atattcacga
     2821 tcagagcgaa cggcgctttg tggtggaatg tgacggcgaa aaagccggtc tggtggagct
     2881 ggtggaaatt aaccatgttc atcgccgcgc agaatttcag ataattatct ccccggagta
     2941 tcaggggaaa ggtctggcaa cccgtgccgc caaattagca atggactatg gcttcaccgt
     3001 tctcaatctc tataagctgt atctgatcgt cgataaagag aatgaaaaag cgattcacat
     3061 ttaccgcaag cttggcttta cggttgaagg tgaattgatg catgagttct ttattaatgg
     3121 tcaatatcgt aatgccattc gcatgtgtat attccagcat cagtatctgg cagagcacaa
     3181 aacaccgggt cagactctcc tgaagccgac cgcacaatag cattaataat aatcgatcgt
     3241 atttttgatg gtgtaaaccc gttcgacggc gggttttact ccttcatcaa caatgatcag
     3301 ctgacagtcc accggattgg cgtgactgtc atattcacag ctctgtttta cattaccaac
     3361 tcgttgatta ttcagtaaag taaccgctgt gtaatctaat tttttgatcg ggtccgttga
     3421 tggcgtggcg ctgacagata atgttttgtc gttacttttc gtggttttac ccagtggata
     3481 accctgatca tcatagcgat attccatctg catttgtttg ctgctggctt taatcacgaa
     3541 gccattatca tcggtttccc aactcacccc ggcggaaggt aattccgcta gctggcattt
     3601 tccctgtaaa cgtactctct tctccagcgt ctcggcatca cggtaataat tggcgtccag
     3661 taccagagcg accacggtat tattttccag atccagtaat tcgagtgaat caaaacagcc
     3721 ttcttccgac aaagtccccg aaacacgttt cgtcacttca ccttgctcat ccattaatgt
     3781 ctgagtgaaa tcttttaccg gaccacgcag cggatcaaaa tcgaattcat tagagaaact
     3841 ggccatctca ggagtaaatg aaagtgttac ctctgtgcgg tcacatcctg tgaggaatat
     3901 cgcgagtaag catggtagta atttgtattt cacaacagtc accagagagt agagatgatt
     3961 ctcaatcata gtagcaaata cagtacttta cacgttaaat gctatgctta aagaagttat
     4021 cttcgcgtaa ggagcttatg atgaaactct caacctgctg tgccgcgctt ctgctcgccc
     4081 tcgcctcacc cgcggtactt gccgcgccgg gatcctgtga gcgcatacag agcgatatat
     4141 cacagcgcat tatcaataat ggcgtacccg aaagcagctt cacgttaagt attgtaccca
     4201 atgaccaggt tgatcagcct gattcccagg tcgtcgggca ttgtgctaat gatacgcata
     4261 aaattctcta cacccgcacc accagcggta acgtctctgc tcccgcgcag tctacccagg
     4321 atggcgcgcc tgccgaaccg cagtaacaca ttatcgactg aacgccggat atgacacatc
     4381 cggcgatttg aacatacaac ataatcccac catattactc atacccttct attgatgtgg
     4441 attaataatt cttaacccaa aatgggtaga cttcctctat tgttagcgcg ctaaatattc
     4501 aatatataaa catttatata acgataaaga acagggagtg agttatgtca aaaaatgacc
     4561 gaatggtggg catcagccgc agaacactcg ttaaatctac cgcgataggt tctctggcgc
     4621 tggctgcagg cggtttttct ttgccgttta ccctgcgcag tgcagcagca gcggtacaac
     4681 aggcccgcga aaaagtggtc tggggtgcct gttccgtcaa ctgtggtagc cgctgtgcac
     4741 ttcgtctaca tgttaaagat aatgaagtga cctgggtgga aactgacaat accggcagcg
     4801 atgagtacgg caaccatcag gtacgcgcct gtttgcgcgg tcgctccatc cgccggcgta
     4861 ttaatcatcc cgatcgcttg aattacccaa tgaaacgcgt gggtaaacgc ggtgaaggca
     4921 aattcgaacg gattagctgg gatgaagccc tggatactat cgccagtagc ctgaagaaaa
     4981 ccgtcgaaca atatggcaat gaggctgtat atattcagta ctcttcgggg atcgttggcg
     5041 gcaatatgac ccgctcttcg ccatcagcct cggcggtcaa acgcctgatg aactgctacg
     5101 gcggttcact caatcagtat ggctcctaca gcaccgcaca aatttcctgt gccatgccct
     5161 acacctacgg cagtaatgat ggcaacagca ccacggatat tgaaaacagc aagctggtcg
     5221 tgatgtttgg taacaacccg gcagaaaccc gcatgagcgg cggtggcatc acttatcttc
     5281 ttgaaaaagc gcgcgagaaa tcgaacgcca aaatgattgt tatcgatccg cgatataccg
     5341 atacggctgc tggtcgtgaa gatgaatggc ttcctattcg cccgggcacc gatgccgcgc
     5401 tggttgcggg gattgcctgg gtattgatta acgaaaatct cgttgatcaa ccttttctcg
     5461 ataaatactg cgtcggctat gacgaaaata ccttacccgc agatgcaccc aaaaatggtc
     5521 actataaagc ctatattctt ggtgaaggag acgataaaac agcgaaaacg ccgcagtggg
     5581 cttcgcaaat taccggtatc ccggtggacc gtatcatcaa actggcgcgt gaaattggca
     5641 cagcaaaacc cgcttatatc tgccagggct gggggccaca acgccaggca aacggcgagc
     5701 tgaccgcacg cgctattgct atgttgccta ttctgacggg caatgtcggc atcagcggcg
     5761 gaaatagtgg cgcgcgtgaa tcgacctata ccattaccat agaacgcctg ccggtgctgg
     5821 ataatccggt caaaacgtca atctcctgct tcagctggac agatgctatc gatcatggcc
     5881 cgcaaatgac ggcaatccgc gacggcgtcc ggggcaaaga taaactggat gtgcccatta
     5941 agttcatctg gaactacgcg ggaaataccc tggttaatca gcattctgac atcaacaaaa
     6001 cgcatgaaat tctgcaggac gaatcgaaat gcgaaatgat tgtggtcatc gaaaacttta
     6061 tgacctcatc ggcaaaatat gccgacattc tgctgccaga cctgatgacc gttgagcagg
     6121 aagatattat tcctaacgac tacgccggta acatgggata tctcattttc ctccagccag
     6181 tcaccagcga aaaattcgaa cgtaaaccga tttactggat cctgagtgaa gtcgcgaaac
     6241 gtcttggacc tgacgtctat caaaagttca ctgaaggtcg cacgcaggaa caatggttac
     6301 aacatctgta cgccaaaatg cttgccaaag atccggcgtt accgtcttac gacgaactga
     6361 aaaaaatggg tatctataag cgtaaagatc ccaatggtca ttttgtcgcc tacaaagcat
     6421 ttcgtgacga ccccgaggca aatccactta aaacaccttc cggtaagatt gaaatttatt
     6481 ccagcaagct ggctgaaatt gcccgtacct gggaactgga aaaagatgaa gtgataagcc
     6541 cactgccagt ttatgcctct acctttgaag gctgggactc acctgaacgt agaaccttcc
     6601 cactgcaact gtttggtttc cattacaaat cccgtactca ctcgacctac ggcaatattg
     6661 atctcctgaa ggctgcctgc cgtcaggagg tgtggatcaa ccctatagat gcgcagaaac
     6721 gtgggattgc caacggcgat atggtgcggg tgtttaacaa tcgtggcgaa gttcggctac
     6781 cagccaaagt gacaccacgt attctccctg gagttagcgc tatgggccag ggagcctggc
     6841 acgaggccaa tatgtctggc gataaaatcg accatggagg ctgtgtgaat acgctaacca
     6901 ctctgcgccc ttcaccactg gcgaagggaa acccgcagca tacgaatctg gtcgagatcg
     6961 aaaaaatata acccactaca accataaatt ctggcatgac attttgtttg aaaagcaata
     7021 agtgagtaat gatgaaaatc cataccacag aggcgctcat gaaggctgaa atcagccgta
     7081 gaagtctgat gaaaacctcc gcacttggca gtcttgcgct ggcaagcagt gctttcactc
     7141 tgccattttc ccaaatggtc cgagcggcag aggcaccggt agaagagaaa gcggtctgga
     7201 gttcctgcac cgttaactgc gggagccgct gtctgttacg tttgcatgtg aaagatgaca
     7261 ccgtgtactg ggtggagtct gatacgacag gtgacgacgt ctacggtaat catcaggttc
     7321 gtgcgtgttt acgcgggcgc tctatccgcc gacggatgaa tcatcctgat aggttgaaat
     7381 atcccatgaa gcgcgtcggc aagcgcggtg aaggtaaatt tgaacggata agttgggacg
     7441 aagccctgga taccatcagt gataatcttc ggcggatcct gaaagattac ggcaatgagg
     7501 ctgtacatgt cctgtacgga acaggcgtag atggcggaaa catcaccaac tcaaacgtcc
     7561 cgtaccgtct gatgaactca tgtggtggtt ttctcagtcg ctatggcagc tacagtaccg
     7621 cacagatcag tgccgcaatg agttatatgt tcggtgccaa tgatggcaac agcccggatg
     7681 atatcgccaa tacgaaactg gtcgttatgt tcggaaataa cccggcagaa acacggatga
     7741 gcggcggtgg tgtcacttac tacgtcgagc aagcccgcga acgttcaaac gcacgcatga
     7801 tcgtcatcga tccacgttat aacgacacag ctgccgggcg tgaagatgaa tggctgccca
     7861 ttcgccctgg caccgatggc gcactggcct gtgcgattgc ctgggtactg attactgaaa
     7921 acatggtcga tcagccattt ctcgacaaat attgtgttgg ttacgatgaa aaaacgctgc
     7981 ccgccaacgc accacgtaac gcgcattata aagcctatat tctgggcgaa gggcctgacg
     8041 gcatagctaa aacgccggaa tgggcagcaa aaatcaccag catcccggca gaaaaaatta
     8101 tccagttggc acgagagatc ggttcagcaa aacctgctta tatttgtcag ggttgggggc
     8161 cacaacgaca ctccaacggc gaacaaacat cccgcgctat tgccatgctt tccgttctca
     8221 ccggtaacgt cggcataaac ggcggcaact caggcgtacg cgaaggtagc tgggatctgg
     8281 gggtagaatg gttcccgatg ctcgagaatc ctgttaaaac gcagatttcc gtctttacat
     8341 ggacagatgc catcgaccat ggtacggaaa tgaccgcgac ccgtgatggt gttcgtggaa
     8401 aagaaaaact ggatgtcccc atcaagtttt tatggtgcta cgccagtaac acattgatca
     8461 atcaacatgg cgacatcaat cacacccatg aggtgcttca ggatgacagc aagtgcgaga
     8521 tgattgttgg cattgaccac ttcatgactg cctcggctaa gtattgcgat atcctgttgc
     8581 ccgacctgat gccaacagag caagaagacc ttatctctca tgaatctgca gggaatatgg
     8641 gctatgtgat cctcgcccaa cccgcaacct cagcaaaatt tgaacgcaaa cccatctact
     8701 ggatgctgag tgaagtcgcc aaacgcttag gaccagacgt ttatcaaacc tttactgaag
     8761 gtcgcagtca gcatgaatgg atcaaatatc tccatgcgaa aacgaaggaa cgtaaccctg
     8821 agatgcccga ctacgaggag atgaaaacga ccgggatctt taagaaaaaa tgcccggaag
     8881 aacactacgt cgctttccgc gcattccgtg aagatccaca ggcaaacccg ttgaaaacac
     8941 cttcggggaa aatcgaaatt tattctgaac gactggcgaa gattgcagat acctgggaat
     9001 tgaaaaaaga tgaagttatt catccccttc cagcttatac gccaggtttt gatggctggg
     9061 acgatcccct gcggaaaacc tatccactgc agttaacggg cttccactac aaagcgcgta
     9121 cccactccag ctacggcaat attgatgtgt tacagcaggc ctgcccacaa gaggtgtgga
     9181 tcaaccccat tgatgctcag gcacgcggta tccgtcatgg cgataccgtg cgggtattta
     9241 acaataatgg agaaatgctg attgccgcaa aagtgactcc gcgtattctg cctggcgtta
     9301 ccgccatcgg gcaaggtgcg tggcttaaag cggatatgtt tggtgaccgg gtcgatcacg
     9361 gcggcagtat taatattctg acctctcacc gcccttcacc gctggcaaag ggaaacccgt
     9421 cgcacagcaa tcttgtccag atcgaaaagg tttaaggagt agcccatgac cacacaatat
     9481 ggatttttta tcgattccag ccgctgtact ggctgtaaaa cctgcgaact ggcgtgcaaa
     9541 gattttaaag atcttggccc ggaagtcagt ttccgccgta tttatgaata tgctggcggc
     9601 gactggcagg aagataatgg cgtctggcac cagaacgtgt tcgcttatta tctctccatc
     9661 tcctgtaacc attgcgatga ccccgcatgt acaaaagtct gccccagcgg cgcaatgcat
     9721 aagcgtgaag atggctttgt ggtggttgat gaagatgttt gtatcggctg ccgctactgc
     9781 cacatggctt gtccgtacgg cgcgccacag tacaatgctg aaaaaggaca catgacgaag
     9841 tgcgatggtt gttattcgcg cgtcgccgag gggaaacaac ccatatgtgt cgaatcctgc
     9901 ccactgcgtg cacttgagtt cggtccaatt gaagagctgc gccagaagca cggcactctt
     9961 gctgccgtcg cgccactgcc gcgcgcgcat ttcacaaaac ccaatatcgt tatcaaacct
    10021 aacgccaaca gccgcccgac cggcgatacc actggttatc tggctaatcc ggaggaagtg
    10081 taaaatggga aatggatggc atgaatggcc actggttatc tttaccgtat tgggtcaatg
    10141 cgtagtaggc gcgctgattg ttagcggaat tggctggttc gccgccaaaa atgacgcaga
    10201 ccgtcagcgt attgtccgtg gcatgttttt cctctggctg ttgatgggca tcgggtttat
    10261 tgcctctgtc atgcacctgg gatcgccact gcgagcgttt aactcgctga atcgaatcgg
    10321 agcttctggt ctgagtaacg agattgctgc cggttccata ttttttgccg ttggcggact
    10381 atggtggctg gtggcggtca tcggtaaaat gccgcaagcg ttggggaaac tctggttact
    10441 tgtcagcatg gcgctcggcg tcattttcgt ctggatgatg acctgcgtgt atcaaatcga
    10501 caccgtgcca acctggcata acggttacac tacgctggct ttcttcctga cggtattgtt
    10561 gagcggtcca atacttgctg ccgcaattct acgggcagct cgcgttacat ttaataccac
    10621 accatttacc atcattagcg ttctggcgct gatcgcttgt gccggggtga ttgtgctgca
    10681 aggtttgtct ctggcgtcta ttcactcatc cgtgcaacaa gccagcgcac tggtaccaga
    10741 ttacgcctcc ttacaggtct ggcgtgtggt attgttatgc gcaggtcttg gatgctggct
    10801 ttgcccgctc attcgtcgcc gtgaacctca cgttgccgga cttattctgg ggctaatcct
    10861 catacttggc ggtgagatga tcgggcgtgt gctcttttat ggcctgcaca tgacagtagg
    10921 aatggctatt gcaggttaac aaattggtgc ggggcaaccc gcacatttca ggatgttagg
    10981 aatgacccat ttttcacagc aagataattt ttctgtcgcg gcgcgcgtgt tgggcgcgct
    11041 gttttattac gctccagaga gtgcggaagc cgcacctctg gttgcggtac tcacccgtga
    11101 cggctgggaa actcagtggc ctctaccaga agcgtcatta gcgccgctgg tgacggcttt
    11161 tcagacacag tgtgaagaga ctcacgccca ggcctggcag cgtttgtttg tcggcccgtg
    11221 ggcactgccg tctccgccgt gggggtcggt ctggctggat cgcgaatctg tgctgtttgg
    11281 cgattcaaca ttggcacttc gtcagtggat gcgcgagaaa ggcattcagt ttgaaatgaa
    11341 gcaaaacgaa ccggaagatc attttggttc actgctgttg atggctgcgt ggttggcgga
    11401 gaatggtcgc cagacagaat gcgaagaatt gctggcatgg cacctttttc cctggtcaac
    11461 acgttttctt gacgttttta tcgaaaaagc agaacaccct ttctaccgtg cactgggtga
    11521 actggctcgc ttaacgctgg cgcaatggca atcacaactg ttgattcctg tcgcggttaa
    11581 accactcttt cgataggatg accgtaatat tgccggatag tggcgattac gcgcaattcg
    11641 gcaataatag gttacagtgt cacgtttttt gtctcttaaa gcacgcactg cttttgcagc
    11701 tggcctcttt tgccgcaaaa tagtcgcccg tgtttcattg cccatttctg ctcatgcatc
    11761 atctacacac ctatccggat ctgcgcacta tgttccgccg tctgcttatc gcaacagtcg
    11821 tcggtattct cgcggccttt gccgttgccg ggtttcgtca tgcgatgctg ctactggagt
    11881 ggttgttcct caataatgac tccggcagtc tggtcaatgc agcgacaaac ctttccccct
    11941 ggcgacggct gctaactccg gcgctcggcg gactggcggc gggtttgttg ctgatgggct
    12001 ggcagaaatt tacccagcaa cgccctcatg cgccgaccga ttacatggaa gcgttgcaaa
    12061 ccgatgtgca gtttgattac gcagcaagcc tggttaaatc gcttgcctct ctgctggtag
    12121 taaccagcgg cagtgcaatt ggtcgcgaag gtgcgatgat tcttttagct gcccttgccg
    12181 cctcctgttt tgcccaacgt tttacgccac gccaggagtg gaaattatgg atcgcctgtg
    12241 gggccgcggc gggaatggct gcggcctatc gtgccccgct tgctggcagt ttatttatag
    12301 ccgaagtgct gtttggcact atgatgttgg cctctctcgg cccggtgatt atttccgccg
    12361 tcgtggcatt gctggttagc aatctgatta atcatagcga cgcgttactc tacagcgtac
    12421 aactctcagt gacggttcag gctcgtgact atgcgctgat tatcagtaca ggtgtgctgg
    12481 caggtctgtg cggaccactg ttgttaacgt taatgaacgc ctgtcatcgt ggatttgtga
    12541 gtctcaaact tgcgccgccc tggcaactgg cactgggcgg gttgatcgtg ggtctgcttt
    12601 ccctgttcac acctgcagtg tggggcaacg gctatagcac cgtacaatcc tttttaaccg
    12661 ccccgccact gttaatgatc attgccggga tcttcctttg taaactgtgt gccgtgctgg
    12721 cgagtagcgg ttccggcgca cccggtggtg tctttacacc gacgctattt atcggtcttg
    12781 ccattggcat gttgtatggt cgtagcctgg gattatggtt ccctgatggc gaagaaatta
    12841 cgcttttact cggattgacc gggatggcga cactgttggc ggcgaccacg cacgcgccga
    12901 ttatgtcgac gttgatgata tgtgaaatga ccggggagta tcagctactc cccggtttat
    12961 tgattgcctg cgtaattgcg tcggtaatct cgcggacgtt acaccgtgag tctatctacc
    13021 gccagcacac tgcgcagcat agctaagcgg atgtattgcc ccagttcacg ctgttcggcg
    13081 cgcggcagat aaggcagttc accaatgagc ggtgccggaa gttttttacc gagcacatca
    13141 atgatttccg cataatgcgc cagtcctggg ttgattcggt tagccaccca gccaatgagc
    13201 ggcagcccgt cgttggcaat cgcctgagct gttaggagtg catggttaat gcaaccttcc
    13261 tgaataccga caaccatcaa caccggcagt tgttcctgca ccacccattc agagagtgga
    13321 cgcaaatcat tcatcagact gcgccagccg ccagtccctt ctaccacgac atgatcgact
    13381 ttatcggtca ggtttgccag gccgtttgaa atgagggtgt aattgattgg gcaactgtgc
    13441 gccacgctac tttcttcttc gcttaacgcg ataggattaa ctgcttcata aggcagttcg
    13501 atggttgaaa cactctgcaa caccagggca tctttattac gcagcccttc gggtgtctct
    13561 ttgctcccct tcgctacggg tttatatccc gcaaccgttt ttccctggga ggctaacgct
    13621 tgtagcaatg cgcgggaaac caccgttttc cctacagaag tgtctgtacc ggtaataaag
    13681 aaacgcttca gcatcactaa ctccaccgtt atgcttcaca aatataaacc aggaaaataa
    13741 ttaaccttga aagtctaagt tatgctttcc tggcccaaat tgagatagcg caaattttgg
    13801 tagaacagtt aaaaaatgtt aaccctgcaa cagacgaatc aacaaagaac cgttatacat
    13861 cgcgtctttt accagcgcag cgcctgccat cgtgccctgg ttagagaact gagtactctc
    13921 aacgctaatg tgctgactat acgcaggaag ggcctgctga cggatgctgt ctgagatcac
    13981 cgggaagagg atatctgccg ctttacttaa cggtgagcca atcagtattt tttgtgggtt
    14041 aaataaattc accatgatgg caagaatgcg ccctacatgc gcgcccaccc cggtaatgat
    14101 gtcttttgcc agcagatcgc cgcgcaatgc cgcctgacac aatgagtcca cggttaacgg
    14161 ttgcccatgt aacatcgagc tcatggattg attgagacgc agctgtgcca gctcaagaat
    14221 actgtccaca ctggcgatgg tttcgaggca gccgtggttc ccgcaataac aacgtttccc
    14281 atacgggtcg acctgtgtgt ggcctatttc cacgaggcta ctgctgcctg cgtgtagcag
    14341 atgaccatcg gtaatgacgc ccgcccccac gttgtgatcg ataaccacct gaatcacatc
    14401 gcgcgccccg cgtgaggcac caaacaaggc ctctgccatc gtccatgcgc tgatatcatg
    14461 ctgaatataa accggaacgc cggtatgctg ctccagagcc tcgccgagcg gcatctcttt
    14521 tacatcctcg tagaacggca tgcgatgtac aatgccattt tccgtatcaa taattcccgg
    14581 cagggttatg gcaatcgaag ttagacgctc aagttttttc tggtggcgga taaaaaactg
    14641 atcgatatgg gaaatgatac gatccagcaa tggcgagtca tcttttaacg ccagttcctg
    14701 cgactcttcc accaccagtt tgctgctcag atcgcgcaga gcaaggaaaa tctccccgcg
    14761 actaatgcgc agagaaagat agtgccaggc ttcagtttca accaccagcc ccaccgccgg
    14821 acggccacgg ttccccgctt ctttgatttc cagctcttgc accaggtgtg cttcgagcat
    14881 ctcacggaca attttagtga tactggcagg agccagctgc gccagacggg aaagatcgat
    14941 acgcgagact ggaccaagct gatcaatcag gcgataaacc gcgcccgcgt tggtctgctt
    15001 tatttgatca atgtgcccag gctggttttc agcaaccacc gcatactccc tatattttcg
    15061 cgctccgaaa taatctgtag gctatggtga agcacttcaa tacgtgtcgt caaattttta
    15121 cttaggcatg tgattaacag cacatttttc gggctttttc gctgaaattt ccctcacctg
    15181 agagcattca gcagatgaat acgaaagtta cgcgcagctg gactttgttc atgatgtttc
    15241 ggccagacca accacatttc agaaaccgca tcctcttcag caatcggcac ccagcgcatt
    15301 tcgttgagct gaacacgttt aaatgacgca ggcaaaattg aaacacccag accggcggaa
    15361 accagaccga tgatggtcat tgcctcgccc acctcctgag tgatgacggg tgtcaaatgg
    15421 taacgtcgca tcagtccgag aatatcgtca tacagccctg tcccgacgtg cggatcaaaa
    15481 aagacaaagg gttctttcgc cagttcagcc agcgttacat tcgggttatt tgccagggga
    15541 tgatcgtgcg ggatcatcgc cataagcggt tcatggacga tgactgcgtg ttcaagcgtc
    15601 tccggtaacg ctgtgttacg cagcaatccc atatccagcg ttccttcaat gagcggagcg
    15661 atttgctcgc gagtgttcat ttcgcgggtt tgtaaatgga catcaggata atctcggcga
    15721 aacagcgata acgtatcaga caccgcccga ataaaaggag cggacgaagt aaaaccaata
    15781 cgcaactccc ccgcttcacc ctgatgcagc ctttcagcgc gagcggcagc gtcatccacc
    15841 atagacagga tttgccgact atctgcaaga aactgttttc ctgctgccgt cagcaataca
    15901 ctgcgattgg ttcgtgccag cagtcgggca ccaatttgtt gctccagcgc ctgaatctgc
    15961 tgacttagcg gcggttgcga aatattcagg cgggcagcgg cgcgcccgaa atgcagctct
    16021 tccgcaacag caacaaagta acgcagatga cgaagttcaa tattcatatt taaaacatct
    16081 tatttgagat tattaatata ttagacagaa caattcgatt ttcctaacct atgtataagc
    16141 ctgatccaca ggcatattta gcaaggattt caagtgagcc gtactacaac tgttgatggc
    16201 gctccggcaa gcgacactga caagcaaagc atttctcagc caaatcaatt tattaaacgc
    16261 ggtacgccgc aatttatgcg cgtcaccctg gcgctgttct ctgccggact ggcaacattt
    16321 gcacttctct attgtgtgca gcctatcctt ccggtgcttt cgcaggagtt tggcttaacg
    16381 cccgcgaaca gtagtatttc actgtccatt tccacggcga tgttggctat tggtttgctg
    16441 tttactggcc ccctatccga tgccattggt cgcaaaccag tgatggtcac ggcgctacta
    16501 ttggcctcca tttgtacgtt actttcgaca atgatgacca gctggcacgg cattttgatt
    16561 atgcgcgcct tgattgggct ttcgttaagt ggcgtggcag ctgttggcat gacttatctt
    16621 agcgaggaaa tccaccccag tttcgtggcc ttttcgatgg ggttgtatat cagcggcaac
    16681 tcaattggcg gcatgagcgg acgcttaatt agtggtgtct tcacggactt tttcaactgg
    16741 cgaattgctc tggcggctat cggttgtttc gcgctagcct cggcgttgat gttctggaaa
    16801 atcctcccgg aatcacgcca ttttcgcccg acttcgctgc gacctaagtc gttgtttatc
    16861 aactttcgtc tgcactggcg tgaccgggga ttaccgttat tgttcgcaga aggctttttg
    16921 ctgatggggt cgttcgtcac gctgtttaat tacatcggct atcggttgat gctctccccc
    16981 tggcatgtca gtcaggccgt ggttggctta ttatcgctgg cttatttgac cggtacatgg
    17041 agctcaccca aagccggaac catgaccacc cgctatgggc gtggtccagt gatgttgttt
    17101 tcgacggggg ttatgctgtt tggtttactg atgaccttat tcagctcgct gtggctgatc
    17161 tttgccggaa tgttactctt ctcagcagga ttcttcgcag cccactcagt tgccagcagc
    17221 tggatcggcc cccgcgcaaa acgcgctaaa ggccaggcct cctcgctgta tctgttcagt
    17281 tactatctgg ggtcgagtat tgccgggacg ctgggtggtg ttttctggca taactatggc
    17341 tggaacggcg tcggcgcatt tattgctctg atgctggtca ttgctctgct ggtcgggacg
    17401 cgtttgcatc gtcgtctgca cgcctgaaaa ataagtccgg actgcagtaa atacccatcc
    17461 ggacttattg cctgctcaaa ccaacgttaa tagccatcct aaaatagacg aagcgccagc
    17521 caattcccgc agcgcgtcta gcgtcatcag gattataagt acccaaataa acggattcat
    17581 tttgctgtgt gtcatttatt actgatgcgc agttattcta ccgctttgta agtagtaaaa
    17641 tagttaaccc gatcaagact actattattg gtagctaaat ttcccttaag tcacaatacg
    17701 ttattatcaa cgctgtaatt tattcagcgt ttgtacatat cgttacacgc tgaaaccaac
    17761 cactcacgga agtctgccat tcccggggat atagttattt caacggcccc gcagtggggt
    17821 taaatgaaaa aacaaattga gggtatgaca atgaaaaaag tattagctct ggttgttgcc
    17881 gctgctatgg gtctgtcttc tgccgccttt gctgcagaga ctgcgaccac acctgctcca
    17941 actgcgacga ccaccaaagc agcgccggcg aaaactacac atcataaaaa acagcataaa
    18001 gcagcacctg cccagaaagc gcaggcggct aaaaagcatc ataaaaatgc gaaaactgaa
    18061 cagaaagccc ctgaacaaaa agcgcaggca gcgaagaaac acgccaagaa acacagccat
    18121 cagcaaccgg caaaacctgc tgcacaaccc gcagcgtaag ttttcaacag taatgctggc
    18181 gcgccccctc gcgcctgaaa attacggtgc taagcgggta acgtttagca ccgcctttag
    18241 cggagggtag tatggtgggc cgttatcgct ttgagttcat tcttatcatc cttattttat
    18301 gcgcactgat tgccgcccgt ttttatcttt cctgactgta gttatctgat tttactccca
    18361 ctttcatccc gtcccgtcta tagtatttat gagggtttgc ttttaataat cataattacc
    18421 caccagagtg tgatatgcgt acaaccattg ctgtagtgtt gggtgcaatt agtttgacgt
    18481 ctgcttttgt gtttgcagat aaaccagacg ttgccaaatc ggcaaacgat gaggtcagca
    18541 ccctgttttt tggtcatgat gatcgtgtgc cagtgaatga cacgacccaa tcaccgtggg
    18601 atgcggttgg gcaactggaa acggccagcg gcaatttatg tacggcgacg ctgattgcac
    18661 ccaatctggc attaacggca ggacactgtt tattgacacc tccaaagggt aaagccgata
    18721 aagcagtagc gctgcgtttt gtgtcaaata aaggtctttg gcgctatgac atccacgaca
    18781 tagaaggccg cgttgatccg acactgggaa agcggttaaa agcagatggg gatggttgga
    18841 ttgtacctcc cgcagccgcg ccgtgggact tcggattgat tgtgctacgt aatccccctt
    18901 ctggcattac gccgttgtcg ttatttgagg gagataaagc cgcgcttacc gccgcattaa
    18961 aagcggcagg tcgtaaagtg actcaggcag gataccctga agatcatctc gatacgttgt
    19021 acagtcatca aaactgtgaa gtgactggct gggcgcaaac gtcggtgatg tcacatcagt
    19081 gcgatacctt gccgggtgac agcggttcgc ctctgatgtt gcataccgat gacggctggc
    19141 aattaattgg ggtgcaaagt tcggctcctg ccgcgaaaga tcgctggcgc gccgataacc
    19201 gggccatttc tgttaccggt tttcgcgaca agctggatca actgtcgcaa aaataatgtt
    19261 caaacgctgc ccgacagcgc gggcagcgtc ttcatcaggc aagtttcacc atgatcattc
    19321 cagccaacag caagaccagg ccaatccagc ctttacgatt taaccgttga ccaaacaaga
    19381 tccaacctgc ggctaacgtg gcggcaatac caaacccgcc ccacaatgca taagcgacag
    19441 acaagtcgat ccctttaacg gcctgagaaa gcgcactaaa ggcagccagc accgccgcca
    19501 gggagagcaa gccaaatatt ttgcgacgaa agccgtcaga aaatttcaaa aagacgttag
    19561 caacgatttc cagcacgatt gccaatgcca gccaggcggc gtgaacccat tcaaactgcg
    19621 ccatggttca cctccagttc aggtttacgc gctttacggg tacctgattt tatcaacaca
    19681 atcccggcga ccagggtggt taacccggca attttcatca gcgataaact ttcgtcgaat
    19741 aacaaaacgc taaacaaggt aataaataaa ataccgatac cttcccacag cgcataagct
    19801 acgcctaagg cgattttttt aacggcgaaa gagagaaata tatacgacag agaaatcatc
    19861 accagcatta aaataaagcc gccatttccc tcactgacgc tcgcccattt catggacagc
    19921 gtaccggtaa tttctgtagc aatagccagg cctaataaaa tccaataaat atacattgtc
    19981 cttctcctgc aagagaatta ttttaatttt cgcttaattc agcgaaacca aagtaaagtg
    20041 gtcaagcccc gcgcttagcg caagctttgg cagaagaaag gactaaagcg cgttgcgcca
    20101 gtgctgctca cctacgagca agatagtgga tgaggtacga agatgggtaa atgtagaaaa
    20161 taacgtcctg aacaaattgt ccataatatt acaattatcc gcagtgttgc ttctcgtcat
    20221 cgcggatgat aattgtcctc ggtagttgaa cacgcctgat ttgtatcata gcttaagaat
    20281 taactcaaaa tattttcact tctttacctg agcggtttga ttttcgttat gatgacggag
    20341 cgaaaaagac attattatta gcaaaggaag aaaaaacgga gacaagcatg gcaaagccga
    20401 tcatcacgct caatggccta aaaatcgtca ttatgttggg aatgctggtc attattctct
    20461 gcggtatccg ttttgccgcc gagatcatcg tgccgtttat tctcgcatta tttattgctg
    20521 ttattcttaa cccgctggtg caacacatgg tccgctggcg agtgccgcgt gtactggcgg
    20581 tgtcgatttt gatgaccatc atcgtgatgg cgatggtgtt gctattagct tacttgggtt
    20641 ccgcgctcaa cgagttgacg cggacgttac cacaatatcg caactctatt atgacgccgc
    20701 tgcaagctct tgagccgttg ttgcaacgcg tagggattga cgtctcagtt gaccaactgg
    20761 cgcattacat tgatccgaac gcggcgatga cgttgctcac caacttattg acgcagttat
    20821 ctaatgccat gtcatcaata tttttattgc tgctgacggt gctgtttatg ctgctcgaag
    20881 tgccacaatt gcccggaaaa tttcagcaaa tgatggcgcg tccggttgaa gggatggcgg
    20941 cgattcagcg tgcgattgac agtgtgtctc attatctggt gctgaaaaca gccatcagca
    21001 tcatcaccgg cctggtcgcc tgggcgatgc tcgccgcact cgatgttcgc ttcgcttttg
    21061 tctggggatt gctggccttt gcgcttaatt acatccctaa tattggttca gttctcgcgg
    21121 caatcccccc tatcgctcag gtactggtgt ttaatggctt ctacgaagcg ttgctggtgc
    21181 tggcgggata tctgctgatt aatctggtct tcggcaatat tctggagccg cgcatcatgg
    21241 ggcgtgggct gggtctttcc acattggtgg tatttttgtc gttgattttt tggggatggt
    21301 tgttaggacc ggtgggtatg ctgctttccg tgccgttgac aattattgtc aaaattgcgc
    21361 ttgaacaaac agcgggaggt caaagcatcg ccgttctgtt aagcgatctc aataaagagt
    21421 gacggcctcc gcagaggccg tcagggttac agagctttca ggattgcatc cacgctggct
    21481 ttggcgtcac caaacagcat gtgggtgttt tctttgaaga acagcgggtt ttgcacacca
    21541 gcatagccag tgttcatcga acgtttaaag acaatcacgt tctgcgcttt ccacacttcc
    21601 agcacaggca taccagcaat cggactcttc ggatcatcct gcgccgccgg gttaaccgta
    21661 tcgttagcac caatcaccag tacggtatcg gtatcagcaa agtcatcgtt gatctcgtcc
    21721 atttccagca cgatgtcata cggtactttt gcttcagcca gcaatacgtt catatgtccg
    21781 ggcaaacgcc ccgcaaccgg gtggataccg aaacgcacgt tgataccacg agcgcgcagt
    21841 ttctcggtaa tttcagcgac aggatattgc gcctgcgcga ctgccatgcc gtaccccgga
    21901 gtaatgatca ctgaatggga gtttttcagc agttccgctg tctcttctgc ggtgatttcg
    21961 cggtgctcac ccacttcctg atcatcgcca gtagaagagc cgtcggtgcc gaaaccaccc
    22021 gcaataacgc tgataaagga acggttcatc gccttacaca taatgtaaga aaggatagcc
    22081 cccgaagaac cgaccagcgc accggtcaca atcagcaggt cgttgctgag cataaagccc
    22141 gcagccgcag ccgcccagcc ggaatacgag ttcagcattg ataccaccac tggcatatct
    22201 gcaccaccga tggaggcgac taaatgccaa ccaaatacca gcgcaatggc ggtcattatc
    22261 agcaatgcca gcacttgcag gccgacgctg tccgtgcgaa caaatacaat cagcagcagg
    22321 aaggaaacga ccagagctgc caggttcatt ttatgacggt ttggcagcat caacggttta
    22381 gacgaaattt tgccacacag tttgccgaac gccaccaccg aacccgtgaa cgttaccgcc
    22441 ccgatgaaga taccgaggaa cacttccgtc aggtgaatat tgaccagaat cggtgccatt
    22501 cccgcgtcat gatgcagata gctgttaaag ccaaccagca ctgccgccag acctacgaag
    22561 ctatgcagga tcgccaccag ttctggcatt tcggtcattt caactttctt cgccagacgg
    22621 ataccaattg ccccaccaat gaccatcgcc agcaagatcc agccaacgtt acccgtatcc
    22681 ggtccaaaaa tggtggcgat tagcgcaatc gccatcccgg cgataccgaa gttgttaccc
    22741 tggcgagacg tttcatgttt cgaaagcccg gccagactga agataaacag gatcgcggca
    22801 acaatgtatg cagctgtaac taatcctcca gacatatgtt accccttaat ttttgcggaa
    22861 cattttcagc atgcgctgag tcacggtgaa gccaccgaaa atattaatgc tggctataag
    22921 caccgcgata aaactaagga agctaaccca gccgccctgg ccaatctgca acagtgctcc
    22981 gacaacaata atccctgaaa tcgcgttggt gaccgacatc aacggtgtat gcagcgcgtg
    23041 cgatacattc cacactacgt aataaccgac aacgcaggcc agcgcaaaaa cggtgaagtg
    23101 cccaagaaat tctttcggcg caacgcttgc cagccagccg aaaaggatga ttgccagcgc
    23161 catcaacgcg tatttacgcc acggtgaaca ggcacatttt tcctcagttt tcacttccgg
    23221 tgccgctttt tgtgccgcct gcggctgagc tgatacctga atcggcggtg ccggccaggt
    23281 aatttcgccc gcacggatca cggtcacgcc gcgaatcacc acatcatcaa aatcaacagt
    23341 gatgttgccg tctttctctt tacacaacag tttcagcaga ttaacgaggt tagtgccgta
    23401 aagctgtgag gattgcgtcg gcagacggcc cggaagatcg gtataaccaa tcactttgac
    23461 accattttcc gtagtgaaga tttcacccgg cacggtgtat tcacagttgc caccgttttg
    23521 ggctgccagg tcgacaatca cactgcccgc cttcatggag tcaaccattt cacgggtaat
    23581 tagcttcggc gctggtttgc ctggaataag cgcggtggtg acaatgatat cgacctcttt
    23641 tgcctgggcg gcaaagagtt ccatttccgc tttgatgaac gcgtccgaca tcactttggc
    23701 atagccatcg ccgctgcccg cttcctcttt aaaatccagc tcgaggaatt ccgcgcccat
    23761 actttgaact tgttctttca cttccgggcg ggtgtcgaat gcacgcacaa tcgcgccgag
    23821 actgtttgct gcgccaatgg cggccagacc tgcaacaccc gcaccaatca ccatcacttt
    23881 tgccggtggc actttcccgg ccgcagtgat ttgcccggta aagaagcgcc caaattcatg
    23941 tgccgcttca acaatggcgc gataaccggc gatgttcgcc atcgagctta gtgcgtccag
    24001 cgattgtgcg cgtgagatac gcggcacaga atccatcgcc atcacggtca cgttacgttc
    24061 cgcaagtttt tgcattaatt ccggattctg cgcaggccag ataaaactca ccagcgttgt
    24121 cccaggattc agtaacgcaa tttcatcatc taacggcgca ttgaccttca gaatgatctc
    24181 tgactgccag acgctattcc cttctacaat ttcagcgccc gcttgcacaa acgctttatc
    24241 gtcaaaactt gccagttgac ccgcgccgct ctctaccgcg acggtaaaac ccagtttcag
    24301 cagctgttcc actgtttttg gcgttgctgc aacacgggtt tcattggtta accgttctct
    24361 tggtatgcca attcgcatga tattcccttc catcggtttt attgatgatg gtttgcctgt
    24421 gtcaggagcc acacaagccg ctcatgtacg agctaaatgt tactccgtta aaataaatta
    24481 gtaacaaacg ccctataacg tactgaaaat tatgcctgtg atctagcgcc aaaaatcagt
    24541 atttcggcgt gaactcgcaa aatattaacg attcagccgt gatagtggga taaacacctt
    24601 agaacgctgg ataaagactg ataattgtct tcgacggtca ggtaaaacga gacatcgccc
    24661 cggcacgaat cactacttaa cattaaatta acttatacaa ttcagttgct tcagtagtat
    24721 tgatgctgat acggctgttt tttaagcata gacggtcatt tgggcaggat taaaattggc
    24781 ttaaggaatc tgatatgaaa aatgacgcag acagttacac cgtttaaatg caataatcag
    24841 ccacgtttct cgttaataac aataccagta cctggtttgc gcaaggcgaa ggattatttt
    24901 tatgaagctt aagaacaccc tcctggcgtc ggcactgctt tctgctacgg cattctccgt
    24961 taacgcagca acagaactga caccggagca agcggcagcg gttaaacctt ttgaccgtgt
    25021 agtggttacc ggtcgtttta atgctattgg cgaagcggtg aaagccgttt ctcgtcgcgc
    25081 agataaagaa ggtgccgcct ctttttatgt tgtcgacact tctgattttg gtaacagcgg
    25141 taactggcgt gtggtcgctg acctctataa agccgatgct gaaaaagcag aagaaacaag
    25201 taatcgcgta attaacggtg ttgtcgaact gccgaaagat caggctgttc tgattgaacc
    25261 gtttgacacg gtcaccgtcc agggcttcta tcgtagccag ccagaagtca atgatgccat
    25321 caccaaagcg gcaaaagcga aaggtgccta ctctttctac atcgttcgtc aaatcgatgc
    25381 caaccagggc ggcaaccagc gtattactgc attcatctat aaaaaagatg ctaagaaacg
    25441 tatcgtccag agcccggatg tgatcccggc agattccgaa gcaggacgtg cagctctggc
    25501 tgccggtggc gaagccgcga agaaagttga gatcccgggt gttgcgacta ccgcatcacc
    25561 aagttctgaa gtcggtcgct tctttgaaac ccagtcatca aaaggcgggc gttacactgt
    25621 cacgcttccg gatggcacta aagtcgaaga actgaacaaa gcgacagcag cgatgatggt
    25681 cccgttcgac agcatcaaat tctctggcaa ctacggcaac atgaccgaag tctcctatca
    25741 ggttgcgaaa cgtgccgcga agaaaggtgc taaatattac cacatcaccc gccagtggca
    25801 ggaacgtggt aataacctga ccgtcagtgc agatctgtat aaataactat tcagttactc
    25861 ttataaggcg gcttgatgcc gcctttcgca tttttagtca caaatttccc cgcgaccatt
    25921 gcatgcagac acatctcttc gtaaaatccc gcgcttcagt ggcaaactcc atttttatgc
    25981 gttttgccct aatatttatt cttcttatca cgttttaatc actggatatc gatggaaaag
    26041 aaactgggac tgagcgcact caccgcgctg gtattaagct caatgctggg cgcgggtgtt
    26101 ttcagtctgc cgcaaaatat ggcggcagtt gccagcccgg cagcactgct catcggctgg
    26161 ggtattactg gcgctggcat tttattgctg gcctttgcca tgctgatcct cacgcgcatt
    26221 cgtcccgaac ttgacggcgg tatctttacc tatgcccgcg aagggtttgg cgagctaatc
    26281 ggcttttgtt ccgcatgggg atactggctg tgcgcagtca tcgccaacgt ctcctatctg
    26341 gtgatcgttt tttccgcgtt aagctttttt acggacacgc cggaattgcg cctgtttggc
    26401 gatggtaata cctggcaatc gatcgtcggt gcatcggctt tattgtggat tgttcacttt
    26461 ttgatcctgc gcggggtgca aaccgctgcc agcattaacc tggtggcgac actggcaaaa
    26521 ttgttgccgc tgggtctgtt tgttgtgctg gcgatgatga tgttcaaact ggataccttc
    26581 aagctcgact tcaccggact tgcacttggc gtacctgttt gggaacaggt gaaaaacacc
    26641 atgctgatca ccctgtgggt tttcattggt gtggaaggtg ccgtggttgt ttctgcgcgt
    26701 gcgcgtaata aacgtgatgt tggcaaagcg acactgctgg cggttctctc cgctctgggc
    26761 gtttacttgc tggtaacgct gctttcgcta ggtgtggtgg cacgtcctga actggctgaa
    26821 attcgtaacc cgtcaatggc cggtctgatg gtggaaatga tgggaccgtg gggcgaaatc
    26881 atcatcgctg ccggtttgat cgtttccgtt tgcggcgcgt acttaagctg gaccatcatg
    26941 gcagcagaag ttccgttcct ggccgccact cataaagcat tcccacgcat ttttgcgcgc
    27001 cagaacgcac aagctgcgcc atccgcctca ctgtggttga ccaacatctg cgtgcaaatt
    27061 tgtctggtac tcatctggct gaccggttcc gattacaaca cgttgctgac catcgcctca
    27121 gaaatgattc tggtgcccta tttcctggtc ggcgcgttct tgctgaaaat cgcaacccgt
    27181 ccactgcata aagcggtagg tgtcggtgcc tgcatttatg gcttatggtt attgtacgcc
    27241 tctggtccaa tgcacttgct gttatccgtt gttctgtatg cgccggggct gctggttttc
    27301 ctgtacgcgc gcaaaacgca tacacatgat aatgtactga accgtcagga aatggtgctg
    27361 attggcatgc tgttaattgc ctcagttccg gcgacctgga tgctggtggg ataacgttca
    27421 tcccatcgtt ggatagcaaa ggagataacg atgggtcaaa cccagccctt gccaatatta
    27481 attactggcg gaggtcgtcg cattggcctc gccctcgcat ggcatttcat taatcaaaag
    27541 caaccggtga ttgtcagcta tcggacacac tatccagcca ttgatggact gattaatgca
    27601 ggtgcgcagt gtattcaggc tgatttttcg accaacgacg gtgtgatggc gtttgccgat
    27661 gaagtactaa aaagcaccca tggtctgcgt gctattttgc ataacgccag tgcgtggatg
    27721 gcggaaaaac cgggtgcgcc actggccgac gtactggctt gcatgatgca gatccacgtt
    27781 aataccccat acctgctcaa ccatgcgctg gaaagattac tgcgtgggca cggacacgcc
    27841 gccagcgata tcattcactt taccgattat gtggtggagc gcggtagcga caaacatatt
    27901 gcgtatgctg caagcaaagc ggcactggat aatatgaccc gctcgtttgc ccgcaagctg
    27961 gcaccggaag tgaaagtgaa ttctattgcg ccatcgctga tcctgtttaa tgaacatgat
    28021 gatgccgaat atcgacaaca ggcgctgaat aaatcactga tgaaaaccgc gcctggcgag
    28081 aaagaagtga tcgacctggt cgattactta cttaccagtt gctttgtcac cggacgcagt
    28141 ttcccacttg atggcggtcg tcatctgcgt taatgcagct ttatccagca tataatcaac
    28201 acccatgcgc ttatccccca acacgcaaca gaaccaacaa aagcagcggg taagcgcatc
    28261 attccggtga aataccacag cgacaccagg taaacaaaat agggaataat cgaccacatg
    28321 ctaaaaatga tggttgcgcg caaggcttcg atgccacgtt cgctggcaac aatataatgc
    28381 gcgatgagcg caaaggtcgg gaaaagtgga atcagcccgg cgatataata atttttcgtt
    28441 tttgctaaaa caccaatcaa cagcactacc agcgcaccga gcgcggcttt gattaccagc
    28501 cccatctttt taccttaaca tttccataac aagtcatcag tagaatacct gatgaaaact
    28561 tgtttagaaa cgattgatag taagtaaaaa cagcgcggtg tattgtgacg tttttatatc
    28621 taccgtgaat gttatgaaca ctatcgtatt tgtggaagat gatgcggaag tcggttcact
    28681 gattgccgcg tacctggcaa aacatgatat gcaggttacc gtagagccgc gcggcgacca
    28741 ggccgaagaa accattttgc gagaaaatcc ggatttggtg ttactcgaca tcatgctacc
    28801 aggcaaggac ggcatgacca tttgtcgtga tttacgcgca aagtggtctg gaccgattgt
    28861 tcttctaacc tctctcgata gcgatatgaa ccacatcctg gcactggaaa tgggtgcctg
    28921 cgactatatt ctcaaaacga cgccccctgc tgttttgcta gcgcgtttac gtttgcattt
    28981 gcgtcagaat gagcaagcca cactgaccaa aggtcttcag gaaacgtctc tgactcccta
    29041 caaagccctg catttcggca cgttgaccat cgatcccatc aaccgcgtag tcaccctggc
    29101 taacactgaa atctcgctct cgacagctga tttcgaatta ttgtgggaat tagctaccca
    29161 tgccgggcaa atcatggacc gcgatgcatt gctgaaaaat ttacgcggcg tcagttatga
    29221 cggactggat cgtagcgtgg acgtggctat ttcgcggtta agaaaaaaac tgctcgataa
    29281 cgccgcagaa ccttatcgca ttaaaactgt gcgtaacaaa ggctatcttt ttgcgcctca
    29341 tgcatgggaa taagcgatga aaaaactgtt tatccagttt tacctgttat tgtttgtctg
    29401 cttccttgtg atgtctctgc tggttgggtt ggtgtacaaa tttaccgccg aacgcgcggg
    29461 caaacagtcg ctggatgatt tgatgaacag ttcgctgtat ctgatgcgca gcgaattgcg
    29521 agagatcccc ccacacgact ggggtaagac gctgaaagag atggatttaa atctctcttt
    29581 cgatctgcgt gtcgagccat tgagtaaata ccatcttgat gatatttcca tgcaccgact
    29641 gcgtggcggc gaaattgtcg ccctggacga tcagtacacg tttttgcagc gtatcccgcg
    29701 cagccactac gtgctggcag ttggtcctgt tccttatctt tattatctcc atcagatgcg
    29761 attgctggat atcgccctga tcgcttttat tgctatttcc ctcgcctttc cggtgtttat
    29821 ctggatgcgt ccgcactggc aggatatgtt aaaactggaa gcagcggcgc aacgatttgg
    29881 cgatgggcat ctcaatgagc gtatccactt tgatgagggt tcgagctttg aacgacttgg
    29941 tatcgcattt aaccagatgg cggacaatat caacgccttg attgccagca aaaaacagct
    30001 tattgacggt atcgctcacg aactgcgaac accgttagtg cgcctgcgtt atcgactgga
    30061 gatgagcgat aacctgagcg ccgccgaatc ccaggcgttg aatcgtgata tcagtcaact
    30121 tgaagcttta attgaagagc tgctgactta tgcccgactc gatcgcccac aaaacgagct
    30181 tcatcttagc gaaccagacc tgccgttgtg gctgtcaacg catctggcag acattcaggc
    30241 agtaacgccc gataaaacgg tacggataaa aacgctcgcg caaggccatt atgcggcgtt
    30301 ggatatgcgc ttaatggagc gcgtgctgga taatttgctc aataatgccc tgcgctactg
    30361 ccattcaacg gttgaaacca gcctgctact gtcggggaat agagcgacat taattgttga
    30421 ggatgatggc ccagggattg ccccagaaaa ccgcgaacat atctttgaac cttttgttcg
    30481 cctcgatccc agccgggatc gctcaaccgg cggctgcggg ctggggctgg caattgtcca
    30541 ctctatagca ctggcaatgg gcggtacggt taattgtgac accagcgaac tgggtggtgc
    30601 ccgcttctcg tttagctggc cgttatggca taacatcccg caatttacct ctgcttgaca
    30661 ctacgcgcac ggtggtcaag tcaccaccac tgtgctataa attaaggatg ttgtaactaa
    30721 agtggttaat attatggcgc gttacgatct cgtagaccga ctcaacacta cttttcgcca
    30781 gatggaacaa gagctggctg catttgccgc tcatcttgag caacacaagc tattggttgc
    30841 ccgcgtgttc tctttgccgg aggtaaaaaa agaggatgag cataatccgc ttaatcgtat
    30901 tgaggtgaaa caacatctcg gcaacgacgc gcagtcgctg gcgttgcgtc atttccgtca
    30961 tttatttatt caacaacagt ccgaaaatcg cagcagcaaa gccgctgtcc gtctgcctgg
    31021 cgtgttgtgt taccaggtcg ataacctttc gcaagcagcg ttggtcagtc atattcagca
    31081 catcaataaa ctcaagacca cgttcgagca tatcgtcacg gttgaatcag aactccctac
    31141 cgcggcacgt tttgaatggg tgcatcgtca tttgccgggg ctgatcaccc ttaatgctta
    31201 ccgcacgctc accgttctgc acgaccccgc cactttacgt tttggctggg ctaataaaca
    31261 tatcattaaa aatttgcatc gtgatgaagt cctggcacag ctggaaaaaa gcctgaaatc
    31321 accacgcagt gtcgcaccgt ggacgcgcga ggagtggcaa agaaaactgg agcgagagta
    31381 tcaggatatc gctgccctgc cacagaacgc gaagttaaaa atcaaacgtc cggtgaaggt
    31441 gcagccgatt gcccgcgtct ggtacaaagg agatcaaaaa caagtgcaac acgcctgccc
    31501 tacaccgctg attgcactga ttaatcggga taatggtgcg ggcgtgccgg acgttggtga
    31561 gttgttaaat tatgatgccg acaatgtgca gcaccgttat aaacctcagg cgcagccgct
    31621 tcgtttgatc attccacggc tgcacctgta tgttgcagat taacgcccgg ctttcatact
    31681 gccgaccatc tgttctggcc gtacccagct gtcaaactcg gcttcgctaa gataccccag
    31741 cgcaagggcc gcagctttta aggtcagccc ttctttatgc gcttttttgg cgatctccgc
    31801 ggctttgtca taaccaatgt gggtgttaag cgcagtcacc agcatcagcg attcattgag
    31861 taattgattg attcgctcac gattcggttc aatacccact gcgcagtgtt tgttaaaact
    31921 ttccatgcca tctgccagca agcgcaccga ttgcaggaaa ttgtggatca ccattggacg
    31981 gaagacgttc agttcaaagt taccggaagc gccccccatg ttgatcgcca cgtcgttccc
    32041 catcacctga cagcagagca tggttaatgc ctcacactgt gttgggttca ctttccccgg
    32101 catgattgag ctgcccggct cattttccgg gattgagatt tcaccaattc cgcagcgcgg
    32161 gccagaggcc agccagcgga catcattggc gattttcatc agtgacgcag ccaacccttt
    32221 caacgcgccg tgcgcctgaa ccagggcatc acaggtcgcc agcgcttcaa atttgttcgg
    32281 cgcggtaaca aacggtgcac aggtaatgac tgccagttca tctgctacgc gacgcgcata
    32341 ctccggatgg gtatttagtc cagtacccac cgctgtaccg cccagagcca gttccgctac
    32401 gtgaggcagg ctgtattcga tatgtttgag attatgctcg agcatcgcta cccagccgga
    32461 aatctcctgc cccagtgtta gcggcgtggc gtcctgcaag tgggttcgac cgatttttac
    32521 gatatcggca aatgcacggg atttctcatt cagcgtctgt gtcagggttt taagctgcgg
    32581 aatgagttgc ttgcgcagcg ccagcagcgc cgcaacgtgc atcgccgtcg gaaagacatc
    32641 gttggaactt tggcttttgt tcacgtcgtc gttagggtga actttacgtt ccatcccgcg
    32701 cacgccgccg agtaattcac tggcccggtt agccagcact tcgttcatgt tcatgttact
    32761 ttgcgtgccg gagccggtct gccagatagc cagcgggaat tcgtcgtcat gctgtcctgc
    32821 cagtacttca tccgccgcct gccgaatggc gctcgctttc tcttcagaca acaagcctaa
    32881 atcttcatta acttttgccg ctgcgcgctt ggttagcgcc agcgcatgaa tcagtgaggt
    32941 gggcattttc tccgtcgaaa tgcggaaatg ctccagcgag cgttgagttt gtgcgcccca
    33001 cagcttatct gccgggacat caatcgcccc catcgaatct ttttcgctgc gtactgtatt
    33061 catggcctgc tcctcacctg attaattttt tctttctgtt ttgctttcgt taagtaactt
    33121 ttagctcact cattatttac tatttgataa caaatgtttg gtctttcgtg ccatgtaaaa
    33181 aaaccgcccc gaagggcggc tctgtttatt tcacacagcg ggtgcattgt gtgagttgta
    33241 tctgctggaa gaagtcattt cctttatcat ccacaaggat aaacgccggg aaatcttcca
    33301 cttcaatttt ccagatggct tccattccca gttccggata ttcaacacat tccaggctct
    33361 taatacttcc ctgcgccaat acagcggccg gaccaccgat actgccaagg tagaagccgc
    33421 cgtgtttttt acaggcatcc gtcacctgct ggctgcggtt gcctttcgcc agcatgatca
    33481 tacttccgcc ctgcgcttgc agttgatcga cataagaatc catccgtccg gcggtcgttg
    33541 ggccaagaga accggaggca taaccttccg gcgttttggc cggacccgcg tagtaaatcg
    33601 gatgatcttt aatgtactgc ggcagccctt caccgttatc catccgctct ttcagtttgg
    33661 cgtgagcaat atcacgaccg acgataatcg tgccgttaag cgataagcgt gtagaaacgg
    33721 gatactgcga caactgtgcg aggatctctt tcatcggacg gttaaggtca acgcgcaccg
    33781 cttcgccctc tcccgctttg cgcagctctt ccgggatata tttgcctgga ttatgttcca
    33841 gtttttcgat ccagatcccc tgacggttga tcttcgcttt gatattacgg tcagcagagc
    33901 aggagacgcc cataccgacc gggcaggatg cgccgtgacg tggcaggcga atcacgcgga
    33961 tgtcgtgagc gaagtattta ccaccaaact gcgcacccag accaagattt tgcgcttcga
    34021 tcagcaattc tttttccagt tccacatcgc ggaacgcctg accgtgctca ttcccttccg
    34081 ttggcagttc atcatagtat ttcgcggaag ccagtttcac cgttttaagg ttcgtttctg
    34141 cagaagttcc accaataacg aacgcaatat gatacggagg acaggccgcc gtacccagcg
    34201 tgcgcatctt ctcaaccagg taatttttca gtttccccgg cgtcagtaac gctttggttt
    34261 cctgatagag atacgtcttg tttgccgaac caccaccttt ggcgatacag aggaatttgt
    34321 actcgtcgcc atcaacggca taaagatcga tctgcgctgg cagattggtg ccggtattca
    34381 cctctttata catatccagc ggcgcgtttt gcgagtagcg cagattatct tcgatataag
    34441 tgttatagac accgcgcgcc agcgccgctt catcaccacc accggtccat acacgctgcc
    34501 cttttttacc aacaataatc gcggtgccgg tatcctgaca ggttggcaga acgcctttcg
    34561 ccgcgatgtc ggagttacgc aggaattgca gcgccacata tttatcattt tcgctggcct
    34621 ccgggtcacg cagaatgtcg gccacctgtt gttggtgcgc cggacgcagc ataaacgacg
    34681 catcatgaaa cgcctgacgc gccaacagag ttaacgcttc gggtgcgact ttcaaaatct
    34741 cctgcccttc aaattcagat acgctaacgt gttcgctggt tagcaggtaa tactcagtat
    34801 catctttttt gagtggaaaa ggagcctgat aatgaaaggg tttgtttgac attgttctct
    34861 cacttactgc ctggtttggt tatgctctgg gcgggtgttc cgttgccctg ttaaaagcga
    34921 gtaacaatat cctacacact tttttaacaa aaactgagac tagtacgact ttttgcggct
    34981 ccaggttact tcccgtagga ttcttgcttt aatagcggga ttaatttcca cattaaaaca
    35041 gggattgatc atgcaaaaac tcattaactc agtgcaaaac tatgcctggg gcagcaaaac
    35101 ggcgttgact gaactttatg gtatggaaaa tccgtccagc cagccgatgg ccgagctgtg
    35161 gatgggcgca catccgaaaa gcagttcacg agtgcagaat gccgccggag atatcgtttc
    35221 actgcgtgat gtgattgaga gtgataaatc gactctgctc ggagaggccg ttgccaaacg
    35281 ctttggcgaa ctgcctttcc tgttcaaagt attatgcgca gcacagccac tctccattca
    35341 ggttcatcca aacaaacaca attctgaaat cggttttgcc aaagaaaatg ccgcaggtat
    35401 cccgatggat gccgccgagc gtaactataa agatcctaac cacaagccgg agctggtttt
    35461 tgcgctgacg cctttccttg cgatgaacgc gtttcgtgaa ttttccgaga ttgtctccct
    35521 actccagccg gtcgcaggtg cacatccggc gattgctcac tttttacaac agcctgatgc
    35581 cgaacgttta agcgaactgt tcgccagcct gttgaatatg cagggtgaag aaaaatcccg
    35641 cgcgctggcg attttaaaat cggcgctcga cagtcagcag ggtgaaccgt ggcaaacgat
    35701 tcgtttaatt tctgaatttt acccggaaga cagcggcctg ttctctccgc tgttgctgaa
    35761 tgtggtgaaa ttgaatcctg gcgaagcgat gttcctgttc gctgaaacac cgcacgctta
    35821 cctgcaaggc gtggcgctgg aagtgatggc aaactccgat aacgtgctgc gtgcgggtct
    35881 gacgcctaaa tacattgata ttccggaact ggttgccaat gtgaagttcg aagccaaacc
    35941 ggctaaccag ttgttgaccc agccggtgaa acaaggcgca gaactggact tcccgattcc
    36001 tgtggatgat tttgccttct cgctgcatga ccttagtgat aaagaaacca ccattagcca
    36061 gcagagtgcc gccattttgt tctgtgtcga aggcgatgca acgctgtgta aaggttctca
    36121 gcagttacag cttaaaccgg gtgaatcagc gtttattgcc gccaacgaat caccggtgac
    36181 tgtcaaaggc cacggccgtt tagcgcgtgt ttacaacaag ctgtaagagc ttactgaaaa
    36241 aattaacatc tcttgctaag cttattaaag gcttataaca ccttcaggcg gccagtccgc
    36301 ctgatttcat tttatggata atcattatga ataaatcgct ggtagcggta ggcgtcattg
    36361 ttgcgctagg cgtagtctgg acaggcggcg catggtatac aggcaagaag attgaaaccc
    36421 atctcgaaga catggtcgcg caggcgaacg cgcaactcaa actgaccgct cctgaatcca
    36481 acctggaagt gagttatcaa aactatcatc gcggcgtatt cagcagtcag ctgcaactgt
    36541 tggtgaaacc cattgccggg aaagaaaatc cgtggattaa aagcggtcag agcgtcatct
    36601 tcaacgaatc ggttgatcat ggtcccttcc cccttgccca gcttaaaaaa ctgaacctga
    36661 tcccgtcgat ggcatcaatt caaaccacgc tggttaataa cgaagtaagc aaaccactgt
    36721 ttgatatggc aaaaggtgaa acgccttttg agattaactc gcgcattggt tacagcggtg
    36781 attccagttc cgatatttcg ctcaagccac tgaattacga gcaaaaggat gaaaaagtcg
    36841 cctttagcgg cggcgagttc cagttaaatg ctgacagaga cggcaaagct atctcccttt
    36901 ccggggaggc gcaaagtggt cggatagacg cagttaacga atacaaccag aaagtgcagt
    36961 tgacctttaa taatctgaaa accgacggtt ccagcacgct ggcaagtttt ggtgagcgcg
    37021 taggaaacca aaaactgtca ctggaaaaaa tgaccatttc agtggaaggc aaagaactgg
    37081 cactgctgga aggcatggag atcagcggta aatcggatct ggtcaatgat ggtaaaacga
    37141 tcaatagcca actggattac tcgctaaaca gcctgaaggt acagaatcag gatctgggca
    37201 gcggcaagct gactttaaaa gtcggccaga ttgatggtga agcctggcat cagtttagcc
    37261 agcaatataa cgcgcaaact caggcgctgc tggcacagcc agaaattgcc aacaatcccg
    37321 aactttatca ggagaaagtg acggaagcct tctttagcgc cctgccgctg atgctgaaag
    37381 gcgatccggt gattactgtc gcgccgctaa gctggaaaaa cagtcagggt gaaagtgcgc
    37441 tgaatctgtc gctgttcctg aaagatccgg caacgactaa agaagcgccg caaacgctgg
    37501 cgcaggaagt agatcgttcg gttaaatctc tggatgcgaa actgaccatt ccggtggata
    37561 tggcaactga gttgatgact caggtagcga agctggaagg ttatcaggaa gatcaagcga
    37621 aaaaactggc gaaacagcaa gttgaaggtg catcagcaat ggggcagatg ttccgtctga
    37681 ccaccttgca ggacaatacc atcaccacca gcctgcaata tgctaacggt cagataacgt
    37741 taaatgggca gaaaatgcca ctggaagatt tcgttggtat gtttgcaatg ccggcattaa
    37801 acgttccggt cgtacccgct attccgcagc agtaattcac agccaaaacg ccggagattt
    37861 ttctctccgg cgttattttt tacttcagca taaagtcata ctgaatactc attttcacat
    37921 cccagtaatt caggcgatcg cccagcgtac tgccatcgcg cttttcccgc cctttatcgg
    37981 cagcaagaaa gaaattcatc ccttgtaacg gcccttcgct gacccgatag ttcataccac
    38041 ctaagatacc cgtgccttca atccgcacat ctcgtttact ggattcatag ccgcgagtca
    38101 caatcggtgc aaatgtcagt gtaaattgcg gcgttaaacg atagttgaca cccaattgcc
    38161 aggactgcat tccttcacgg ttacgatcta gacttaatga aaagggatag ccaatgtcgg
    38221 tatccaccac ggcaccatta attgcatggc gcaaataacc accactgcca aatacgcttc
    38281 cccacggcgc attccaggtc aattgtccac taaccaacgc cgtttcattg ggctggctgg
    38341 tattacgact taaaccttcg agctgcgcgt aaaagccgag caactcccct ttcaacgtat
    38401 aattcgggtc gagcggtatg cttttctcga gaatactttg tatctgattg gaaacatctt
    38461 tctgtaccga tgtccccaga gaatagcgac tggtatcatc atgccaactg gcggctaaaa
    38521 aagaacgtgc gcgatgttta tcgtattttc ccgtctggct gttatacacc ccttcatcac
    38581 gaataccggt tgttgaatca gtccataaaa atactcgtga acgccacatt ccccacagat
    38641 caatatttgg cgttaaagcc caataaccta atgctccttc agaaactgtc ggtaatgtac
    38701 gttgcgaact gccagccatt aacggaatac gataaggtaa gctaccataa ctataatccg
    38761 tcccgaagcg acctatttta agcaggctat tttcactggg ttttattttt actgccccga
    38821 gggcaaagcc aaaactttta tcaccatcaa gataccaccg ggtactcata tcagcacgag
    38881 cacctaattt ataaacataa tacgctccgc cttcaacgcc aataatattg gaataataac
    38941 cactattgaa atcgagcaat cctccctgta cccatgcgta aatatctctt cccgcgccag
    39001 cactgggctt atcagccctg cgcaattcgt ttttcagtct gatgcgtagt cccgcttcat
    39061 cgtcagccag ttgcgccgcg taagcagagg taaggccaga ggcagccgtc aggcaaataa
    39121 cggtcatggc
//
LOCUS       DAPGOF010000022        38086 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.022, whole genome shotgun sequence.
ACCESSION   DAPGOF010000022 DAPGOF010000000
VERSION     DAPGOF010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38086
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.022"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(78..365)
                     /locus_tag="RHV13_001013"
     CDS             complement(78..365)
                     /locus_tag="RHV13_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HDV1240568.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVEDEDIIRLVAER"
     gene            complement(440..583)
                     /gene="mgrB"
                     /locus_tag="RHV13_001014"
     CDS             complement(440..583)
                     /gene="mgrB"
                     /locus_tag="RHV13_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HDV1240569.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            742..981
                     /locus_tag="RHV13_001015"
     CDS             742..981
                     /locus_tag="RHV13_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1240570.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCIYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(1125..1916)
                     /gene="kdgR"
                     /locus_tag="RHV13_001016"
     CDS             complement(1125..1916)
                     /gene="kdgR"
                     /locus_tag="RHV13_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HDV1240571.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            2093..3466
                     /locus_tag="RHV13_001017"
     CDS             2093..3466
                     /locus_tag="RHV13_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1240572.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            complement(3512..4393)
                     /gene="htpX"
                     /locus_tag="RHV13_001018"
     CDS             complement(3512..4393)
                     /gene="htpX"
                     /locus_tag="RHV13_001018"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HDV1240573.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(4585..6633)
                     /gene="prc"
                     /locus_tag="RHV13_001019"
     CDS             complement(4585..6633)
                     /gene="prc"
                     /locus_tag="RHV13_001019"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HDV1240574.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(6653..7351)
                     /gene="proQ"
                     /locus_tag="RHV13_001020"
     CDS             complement(6653..7351)
                     /gene="proQ"
                     /locus_tag="RHV13_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HDV1240575.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTAKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(7448..7945)
                     /gene="msrC"
                     /locus_tag="RHV13_001021"
     CDS             complement(7448..7945)
                     /gene="msrC"
                     /locus_tag="RHV13_001021"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HDV1240576.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            8075..9358
                     /gene="yebS"
                     /locus_tag="RHV13_001022"
     CDS             8075..9358
                     /gene="yebS"
                     /locus_tag="RHV13_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HDV1240577.1"
                     /translation="MALNTPQITPTKKITVRAIGEELTRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPAARSDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            9327..11960
                     /gene="yebT"
                     /locus_tag="RHV13_001023"
     CDS             9327..11960
                     /gene="yebT"
                     /locus_tag="RHV13_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HDV1240578.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIERKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKARYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            12040..13479
                     /gene="rsmF"
                     /locus_tag="RHV13_001024"
     CDS             12040..13479
                     /gene="rsmF"
                     /locus_tag="RHV13_001024"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HDV1240579.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLAAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPTPKYKVGNFPFSPVKD
                     REAAQIRQAAASVGLNWDENLRPWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNENAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            13597..13833
                     /locus_tag="RHV13_001025"
     CDS             13597..13833
                     /locus_tag="RHV13_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HDV1240580.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            13938..14129
                     /locus_tag="RHV13_001026"
     CDS             13938..14129
                     /locus_tag="RHV13_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HDV1240581.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLHSMSDQRIRQGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(14130..14786)
                     /gene="pphA"
                     /locus_tag="RHV13_001027"
     CDS             complement(14130..14786)
                     /gene="pphA"
                     /locus_tag="RHV13_001027"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HDV1240582.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALGKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLSERQKVQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(15182..15523)
                     /locus_tag="RHV13_001028"
     CDS             complement(15182..15523)
                     /locus_tag="RHV13_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HDV1240583.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(15536..16408)
                     /gene="copD"
                     /locus_tag="RHV13_001029"
     CDS             complement(15536..16408)
                     /gene="copD"
                     /locus_tag="RHV13_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HDV1240584.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGIGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(16412..16786)
                     /gene="yobA"
                     /locus_tag="RHV13_001030"
     CDS             complement(16412..16786)
                     /gene="yobA"
                     /locus_tag="RHV13_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HDV1240585.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            16925..17155
                     /gene="holE"
                     /locus_tag="RHV13_001031"
     CDS             16925..17155
                     /gene="holE"
                     /locus_tag="RHV13_001031"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HDV1240586.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            17257..17913
                     /locus_tag="RHV13_001032"
     CDS             17257..17913
                     /locus_tag="RHV13_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HDV1240587.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASTRR
                     LQFFSHQYSIAVLMANARGNSALWDEHGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            17937..18599
                     /gene="exoX"
                     /locus_tag="RHV13_001033"
     CDS             17937..18599
                     /gene="exoX"
                     /locus_tag="RHV13_001033"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HDV1240588.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(18596..20656)
                     /gene="ptrB"
                     /locus_tag="RHV13_001034"
     CDS             complement(18596..20656)
                     /gene="ptrB"
                     /locus_tag="RHV13_001034"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HDV1240589.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(20865..21524)
                     /locus_tag="RHV13_001035"
     CDS             complement(20865..21524)
                     /locus_tag="RHV13_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HDV1240590.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(21851..22207)
                     /locus_tag="RHV13_001036"
     CDS             complement(21851..22207)
                     /locus_tag="RHV13_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HDV1240591.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(22274..22564)
                     /gene="yebG"
                     /locus_tag="RHV13_001037"
     CDS             complement(22274..22564)
                     /gene="yebG"
                     /locus_tag="RHV13_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HDV1240592.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            22698..23876
                     /gene="purT"
                     /locus_tag="RHV13_001038"
     CDS             22698..23876
                     /gene="purT"
                     /locus_tag="RHV13_001038"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HDV1240593.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEQLQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(23932..24573)
                     /gene="kdgA"
                     /locus_tag="RHV13_001039"
                     /gene_synonym="eda"
     CDS             complement(23932..24573)
                     /gene="kdgA"
                     /locus_tag="RHV13_001039"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDV1240594.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(24610..26421)
                     /gene="edd"
                     /locus_tag="RHV13_001040"
     CDS             complement(24610..26421)
                     /gene="edd"
                     /locus_tag="RHV13_001040"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HDV1240595.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(26656..28131)
                     /gene="zwf"
                     /locus_tag="RHV13_001041"
     CDS             complement(26656..28131)
                     /gene="zwf"
                     /locus_tag="RHV13_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDV1240596.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            28469..29338
                     /gene="hexR"
                     /locus_tag="RHV13_001042"
                     /gene_synonym="yebK"
     CDS             28469..29338
                     /gene="hexR"
                     /locus_tag="RHV13_001042"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HDV1240597.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            29466..30908
                     /gene="pyk"
                     /locus_tag="RHV13_001043"
     CDS             29466..30908
                     /gene="pyk"
                     /locus_tag="RHV13_001043"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDV1240598.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(31039..32010)
                     /gene="lpxM"
                     /locus_tag="RHV13_001044"
                     /gene_synonym="msbB"
     CDS             complement(31039..32010)
                     /gene="lpxM"
                     /locus_tag="RHV13_001044"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HDV1240599.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDDKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(32130..33452)
                     /gene="mepM"
                     /locus_tag="RHV13_001045"
     CDS             complement(32130..33452)
                     /gene="mepM"
                     /locus_tag="RHV13_001045"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HDV1240600.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFMRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(33468..34400)
                     /gene="znuA"
                     /locus_tag="RHV13_001046"
     CDS             complement(33468..34400)
                     /gene="znuA"
                     /locus_tag="RHV13_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HDV1240601.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            34479..35234
                     /gene="znuC"
                     /locus_tag="RHV13_001047"
     CDS             34479..35234
                     /gene="znuC"
                     /locus_tag="RHV13_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HDV1240602.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNAKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            35231..36016
                     /gene="znuB"
                     /locus_tag="RHV13_001048"
     CDS             35231..36016
                     /gene="znuB"
                     /locus_tag="RHV13_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HDV1240603.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMANIRVDLMTYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(36359..37369)
                     /gene="ruvB"
                     /locus_tag="RHV13_001049"
     CDS             complement(36359..37369)
                     /gene="ruvB"
                     /locus_tag="RHV13_001049"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDV1240604.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(37378..37989)
                     /gene="ruvA"
                     /locus_tag="RHV13_001050"
     CDS             complement(37378..37989)
                     /gene="ruvA"
                     /locus_tag="RHV13_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDV1240605.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
BASE COUNT         9110 a         9828 c         9392 g         9756 t
ORIGIN      
        1 tgcaccatga cagtgcgtat aatcaggatg aattgagtaa cccttcaacg acggggttac
       61 tcaagattga ggaaggatta tcgttcggcg accaggcgaa taatatcttc gtcttcaacc
      121 gatttttgac tctcaatggt ctttttatct gctttctctg gttcgtttgc ttcgcacaaa
      181 cgacgtaaca gtgcattttg acgtttttgc tgatccagca acgcctcaag cagttcaatc
      241 tgttcgttag tccgagaact ggcacgatta ataaaaaacc acaagatgag cccgataaga
      301 agaaccacca ccgatacaac caaagacgca atgttcatca cgcctgaatt tacaacttcg
      361 ttcatttcac cacctctgag tagaggcgct attctaccac tgctggagag gaagaaaatc
      421 tagtgctgaa aaaatgatat caccacggga taaactggtt aatggcacaa attccgctga
      481 aaaattgtac atcctgatcg cacatcatgt tgaatacctg cgcccaaagc agcaagcaag
      541 ccaacaccac gacaaccaaa acgacccatc gaaacttttt cactccaatc tccgtttcac
      601 ctaccttatg tcattaaact agcatgcgtt agttaaacag cgcctaacta tgtcgatatt
      661 cgtgcttttt cggaatgagt cgcttgcttc agcgcatatt gccgctacga ttaagcgaac
      721 ataaaaagag aagaggttgt aatgcgattc atcattcgca cagttatgct gatagcactg
      781 gtatggatag gattattact cagcggatat ggcgttttga ttggaagcaa agagaatgcc
      841 gcaggattag gtttgcaatg tatctatctg actgccagag gcaccagcac ggtacaatac
      901 ttgcacacta aaagtggttt tttggggata acagattgcc cgctgttacg caaaagcaat
      961 atcgtggtcg ataatggtta agggcaataa ataaccagat tctcactcct gagtaaaata
     1021 ctcttatagc aagcgcgttt aatataacgc caccttcaac tttatttttc tcatgctaaa
     1081 taaagaacct gtctgtttga tattccgaca ggttcttact ctcatcagaa cggatagtcg
     1141 tgataaccca tttgggcaga aattttgcgc gctgcggtat gcaacattgc gacatattcc
     1201 tgtaaacgct cttcagagaa acgcaacgtc gggaaggaga tgctcaaacc ggcaatgacc
     1261 acgccaaagc gatcaaatac cggaaccgca atgcatcgca gcccttcttc ctgctcttcg
     1321 ttatcttcgc catacccctg ctcgcgcacc tggtccagaa ccggtaataa cgcttctgta
     1381 ctggtgatgg tccgctcggt actgcgttta tactccacgc cctcaagaat ttgcttcact
     1441 tcatcgcgat cgcgccatgc cagcagtacc ttaccaatcg cggtgctgta cagtggatta
     1501 cgacgcccaa tccgtgaata catgcgcaaa ttgtacatag agtcaatttt gtgaatgtaa
     1561 acaatactgt cttcgtccag tgcgccgagg tggatagttt ctttggtcag gcgggagatc
     1621 tcacgcatct ggatatctgc gctacgaatt aaatcgacgt tttgtaacgc gcgagcgccc
     1681 agttcaaaca atttcagggt cagggaatat ttctccgact ccccttcctg cgccacataa
     1741 cctaaggttt tcatggtctg taaaaagcga taaacggtgc tttttgacat catgacgcgc
     1801 tgcgacagct cggttatccc tatttcgcgc tcttcaccca gcgcctgcaa aatgccaaaa
     1861 acttttagca cggaagatac agaatcaggc tgtttatcca gatctgcgtt agccatttat
     1921 cacctcattg cgagtgtttt ataaaaatca gaactgtttt ttattataat ttcgcaccag
     1981 ggtggtcgca atccatcttt tgccggttag ttacaattct gcgacatcca ccgtgaatat
     2041 cagtgctaga atcatacccc tgttgattat tcaccaaaga tttaaaattc ctatgccaaa
     2101 agttcaggcc gacggcctgc cattgcccca gcgatacggt gcgatattaa ccattgtgat
     2161 tggtatttcg atggctgtcc ttgacggcgc aatcgccaac gtcgccctgc caacaatcgc
     2221 cacggacctt catgccacgc cagccagttc catctgggta gtgaacgcct atcaaatcgc
     2281 cattgtcatc tccctgctct cgttttcgtt tctgggcgat atgtttggct atcgacgtat
     2341 ttataaatgc ggtctggtcg tttttctgtt gtcttcactg ttctgcgccc tttctgattc
     2401 gctgcaaatg ctcacccttg cgcgtgtcat acaaggtttc ggcggcgcag cgttgatgag
     2461 cgtaaatacc gcacttatcc gcctgatcta tccacaacgt tttctgggta gagggatggg
     2521 cataaactcg tttattgttg ccgtttcttc tgctgccggg ccgacaattg ctgcagcaat
     2581 cctctccatc gcatcctgga aatggttatt tttaatcaac gtaccgttag gtattatcgc
     2641 cctgcttctc gcgatgcgtt ttctgccacc caatggttct cgcgccagta aaccccgttt
     2701 cgacctgccc agcgccgtga tgaacgcgtt aaccttcggc ctgcttatca ctgcgttgag
     2761 tggtttcgct caggggcaat cgctgacgtt aattgctgcg gaactggtgg taatggttgt
     2821 cgttggtatt ttctttattc gccgccagct ttctcttcca gtaccgctgc taccggtgga
     2881 tttactgcgt atcccgctgt tttcactttc tatttgcaca tctgtttgct ctttctgcgc
     2941 acaaatgctg gcaatggttt ccctgccctt ttacctgcaa accgtgctcg ggcgtagtga
     3001 agtcgaaaca ggtttacttc tgacaccgtg gccgttagca acgatggtga tggctccgtt
     3061 ggcaggctat ttgattgaac gcgtacatgc aggattgctg ggtgctttag gattattcat
     3121 catggctgcg gggctttttt ccctggttct gctgcccgcg tcacctgcgg atatcaatat
     3181 tatctggccg atgatcttat gtggcgctgg atttggcttg ttccagtcac ccaataacca
     3241 caccattatt acctccgctc ctcgcgaacg tagcggtgga gccagtggca tgttaggaac
     3301 ggctcgtcta ctgggtcaga gtagcggcgc ggcgctggtg gcgctgatgc taaatcagtt
     3361 tggagataat ggtacacacg tctcgctgat ggctgcggct attctggcag tgattgctgc
     3421 ctgtgtcagt ggtttacgta tcactcagcc acgatccagg gcataaaaaa agcgcgtcga
     3481 tcagtacgcg ctttttagta tttacttcat attacttcag gtattcaccc gtacgcagag
     3541 cttcaattcg tttatccagc ggcgggtgag tcatgaacaa ctcactgagc gatttcgact
     3601 taccgttaat gcagaaagcc atcatgctgg ttgcttcttg cggttcatag ctggttttca
     3661 ggcgctgcag cgcggcaatc attttctcgc gaccaaccag ttttgccgaa ccggcatctg
     3721 catggaattc acgatgacgc gagaaccaca tggtgataat gctcgccaga ataccaaaca
     3781 ccagttccag aaccgttgca accgcaaagt agatcagcgg gttgccgttg ctctcttcac
     3841 cttcatcacg atttccgccc ataaaacccg cggcaagctg cgccaggata cgggaaataa
     3901 agataacgaa ggtgttcacc acgccctgaa tcagcgtcat ggtgaccata tcaccattgg
     3961 cgatgtggct gatttcgtga gcaattaccg cctcggcttc atccgggctc atgttctgca
     4021 gcaaaccggt gctgacagca accagagagg catcacggcg cgcaccggtt gcaaaagcgt
     4081 tgatgtccgg cgcatggtag atagccactt gcggcatagc gatccccgcc tgacgagcct
     4141 gggttgctac agtattgacc agccaacgtt ccctttcgtt acgcggttgc tcgatcactt
     4201 ccccgccaac agatcgtaat gccatccatt tggacatcag aagcgaaacg aaggaaccac
     4261 caaaaccgaa cagcaaggcc atgatcatca gcccctgaac gctgctcgac tgtatccctg
     4321 tcaggctcag taccagcccg aaaacgacca ttacggccag gttcgttagc aggaagagcg
     4381 cgattcgcat cataattttc tttttacctc agtttaacaa aacgcaatat gcgataccca
     4441 catcgtatgg gttacgcgac tattttcaag tctggatagt gcgtaagtca ccagaaagac
     4501 acaactttac attttgtagc atctgattta cggcatcttg ccgctgttaa aaaatcaggc
     4561 acaatttctt gtgcctgatt gatattactt gacgggtgcg ggttgttccg cgggtctggc
     4621 tttttcaagc ttcgccagat cgagtgcgat attcaccgtc tcatccagat aaggatccgg
     4681 ctcctggtaa tctttcggta gatcatccag tttcttcaac tccggtttac cttcgcgttt
     4741 aaagcgttcg ttcaaacgcg ccagacgcgt cgcatcatct tcattattct ctttctcacg
     4801 cacggcgtaa ttcagagaaa cgatattacg cttgtccttc atagcgttga agcgcgcgat
     4861 atccttcatg atgttctgga actcaggatc tttcgcgata cgcgcattat gttccttcag
     4921 tagctccggt tcaaaggccg ttaaatctcc tgatttcaca taagtcgcgg catcaatgct
     4981 atcccacggc agcgcgttat cttcgaattt ctcacccgtt tccgtttctt cattacccgt
     5041 cggcatgatg atgtctggcg ttacgccttt acgttgcgta ctgccgccgt taacgcgata
     5101 gaatttctgg atcgtgtact gcacagaacc cagcgctggc cattcaggac gtaacatctg
     5161 atcgtaaata cggttcaatg aacggtattg ctgaacggtg cctttaccaa acgtcggttc
     5221 acccacaacc agcgcacgtc cgtaatcctg cattgccgcg gcaaagattt ctgaagccga
     5281 agcactgaag cggtcaacca gtaccaccag cgggccttta tagaaaacct gtccgtcggt
     5341 atcgctgtct tcacgaactt taccgttgtt atcacggacc tgaacaatag gacccgcagg
     5401 aataaacaga cctgagagcg ataccgcttc ggtcaacgcc ccaccgccat tgctacgcag
     5461 gtcgatgatc acactgctga cattctgttt ttccagtttc tgcagttgta ctttgacatc
     5521 gtctgtcaaa cccacataga agcccggaat atccagtacg ccgactttct ctttaccgac
     5581 ggtcttcacc gacattttaa ccgcgcggtc ttcgagacga atacgttcac gggtcaatgt
     5641 tacagtgcgg gtcttggtcc ctttaccagc aggtaaaatt tccagacgaa ctttactgcc
     5701 cttcggccct ttaattaagg caaccacatc atcaagacgc cagccaatca cgtcaaccat
     5761 cggcttgcct gtttgaccaa caccgacaat tttgtcacca acgctgatag ctttactctt
     5821 cgctgccgga ccacctgcca ccatcgaatt gataacggtg tagtcatcat ccatttgcag
     5881 cactgcgcca ataccttcca gcgacaaact catttcagtg ttgaactgtt cggtattacg
     5941 cggggaaaga tagttggtat gcgggtcgat ttcacgcgca aacgccgtca ttgccagcga
     6001 gaaaacatct tcgctgttgg tttgcgccag acgacgaatg gcaaatttgt agcggcgagt
     6061 cagggtttca cgaatttctt tatccgtttt tcctgccagc ttcaggctta actcgtcgaa
     6121 tttgacttta ctgtcccaca gcgcgttcaa ctcagcctcg tttttcggcc agggcgcttt
     6181 gctgcggtca aggttataag tgtcgttgcc ggtgaaatcc atcggctttt ccagtaccga
     6241 caaagcgtac tggtaacgtt caaaacgacg cttttgcgcc agattgtaga gatcgtagaa
     6301 aacgtcgagt ttgcctgaac gcagttcatc gcctaactcg gttttctttt tcgcgaactg
     6361 ttcaacatcg cttgccaaca gcacgttgtg gctgtaatcg agcagattca ggtagcggtc
     6421 aaagattttg gccgaaaatg cctgatcgag gtcaaactgg cgataatgag aacgggtgaa
     6481 gcgcgacgtt acgcgctcac ttaccgtcgc atgctgcgtc tcttccttta ataccggaat
     6541 ttgatcagca cgcgtgatat cttctacagc gaaggtctgg cctgctattg caagcaggcc
     6601 agctaacgcg gtaagcctaa aaaacatgtt catgcctggc ccggcctccg tttcagaaca
     6661 ccaggtgttc tgcgcgcaca atcaaagaca tacccgaatt cagctggacg cggacgccgt
     6721 ctttggtgat ttctaatacg gtggcatcca tcgcgttttg acccgctttc accttcaggg
     6781 cttgtccgac agtcagagct gaaatgtcag aaacaggggt gtgctgttct tcgcgaggtg
     6841 cttttgctgt ttttggcgct ttctctaccg gcttttgcgc acgaggttta cgttcagcgc
     6901 cttctttgcg gcgtggcgtg gtcggacgtg gcttgcgttc gcgacgcggt gcgtcttctt
     6961 tctcaccagc agctgcggca gcttcgcgtt ttttcgcttg ctgttcagca cgctgtgcct
     7021 gaacacgcgc tttcgcttct tcaagctgct tgcgagcatg ctctacatgt tgctcgtcca
     7081 gctcaccgca tgggttgccg tcaagatcga cacgcgttgc gccgggttta acaccgtaaa
     7141 gataacgcca gctcgaagtg tagagacgta aagcggatcg taattgcgtt ttgctcaggt
     7201 tcatttcccc ggcaacacga tcgaccaaat cctgaaaaat accgattttc agcggacgcg
     7261 cttcaccttc cgcactgaaa cagtggggaa aacgttcggc cagaaacgcg attacttctt
     7321 tactgctatt caacttaggt tgattttcca tgaaatttcc tgattacaac ggacgtagcc
     7381 aacaagcgca ggcatgaaca ggcgtcatta taatgacgct accagtaaat gctacgttat
     7441 ccgttgatta tcctgcgacg ctcgcaaaga attttttgta atccgtcgtt gcaagcactt
     7501 tttcaagctg tgccacaagc tgacgtaagc cttgctcgtc ctcgtctgta aagcgaccga
     7561 agacggtact atcgatgtcg agaacaccaa taatctgatt tttcaccacc agcggcagaa
     7621 caatttcaga attactcgcc gcatcacagg caatatgccc gtcaaacgcg tgaacatcct
     7681 cgatacgctg cacttgattg cgggcaaccg cagtgccgca caccccgcgc ccgacgggta
     7741 tccggacaca ggcaattttg ccctgaaatg gtccgagtac cagtgtatcg tcctcaagca
     7801 aataaaaacc tgcccagttt acgtcagtga gacgctcgta taacaacgca ctggtgttcg
     7861 caagcgttgc cagaaaactg gtttctcccg ccatcagcgc gttaaagtcg cgatttaaat
     7921 ccgcgtaaaa ttctgttttg ttcattatat aatcacttgg ttgtcttacc tggaactgcc
     7981 agcctattaa aataagcatt aaatgcgtta atgctcaaga tcattcccat catgggttaa
     8041 gattaatgtt aattcttatt acatttggca cgtcatggct cttaacacac cacaaattac
     8101 gccgacaaaa aagataacag tgagggcaat cggcgaggaa ctgacgcgtg gtgattacca
     8161 acgttgcccg caatgtgaca tgctgtttag cctgcccgag ataaattctc atcaaagtgc
     8221 ctattgtccg cgctgtcagg caaaaattcg tgacgggcgc gactggtcgc taacgcgcct
     8281 ggcggcaatg gccttcacca tgctgttgct tatgccgttt gcctggggcg aaccgctgtt
     8341 gcatatctgg ctgttaggta ttcgtatcga cgccaatgtc atgcaaggca tctggcaaat
     8401 gaccaaacag ggcgatacga taacgggggc gatggtcttt ttctgcgtta ttggtgcccc
     8461 cctcattctg gtgtcctcca tagcatattt atggtttggt aaccgactgg gaatgaattt
     8521 acgtccggta ctgctgatgc ttgagcgact taaagagtgg gtaatgctgg atatctacct
     8581 ggtcggcatt ggcgttgctt ctataaaggt tcaggattat gcccatatcc aggctggtgt
     8641 cggcttgttc tcttttgtgg cgttggtgat tttaacaacg gtgacgttgt cacatcttaa
     8701 tgttgaggaa ctgtgggagc gattttatcc gcagcgcccc gctgcgcgta gtgacgagaa
     8761 actccgtgtc tgtcttgggt gccattttac cggctatcct gatcagcgtg gtcgctgccc
     8821 gcgttgccat atcccgctac gcctgcgtcg ccgtcatagt ctgcaaaaat gctgggcggc
     8881 gctgttagcg tcaatcgttt tgttgttacc tgccaacctg ttgcctattt ctatcattta
     8941 tctgaacgga ggacggcagg aagatacaat tctttccggg attatgtcgc tggcaagtag
     9001 caacattgcc gttgcgggaa tcgtgtttat cgccagtatt ctggtaccgt ttactaaagt
     9061 gatcgtcatg ttcactttac tgttgagcat tcattttaaa tgccagcaag gtttacgcac
     9121 acgcattctg ttactgcgaa tggtgacctg gattggtcgc tggtcgatgc tcgacctgtt
     9181 tgtcatatct ttaaccatgt cgctgattaa tcgcgatcag atcctcgctt ttactatggg
     9241 accggctgcg ttttatttcg gcgcagcggt aattttgact attcttgctg tggaatggct
     9301 ggacagccgc ttactttggg atgcacatga gtcaggaaac gcccgcttcg acgactgaag
     9361 cgcagattaa aaataaacgc cgtatctcac ctttctggct gctgccgttc atcgcgctaa
     9421 tgattgccgg ttggctgatt tgggacagtt atcaggaccg gggtaatacc gtcaccatcg
     9481 actttatgtc ggcggatggt attgtcccgg gccgtacgcc tgttcgttat cagggcgttg
     9541 aagtcggaac agtgcaggat atcagcctca gcgacgatct tcgtaagatt gaagtcaagg
     9601 tcagcatcaa gtccgatatg aaagatgcgc tgcgcgaaga gacacagttc tggctggtga
     9661 cgccaaaagc atcgttggca ggtgtctccg ggctggacgc cctcgtcggt gggaactata
     9721 tcggcatgat gccgggtaaa ggtaaagagc aggatcactt tgtcgcgctc gatacccaac
     9781 cgaaatatcg gctggacaat ggcgatctga tgatccacct gcaagctccc gatctcggtt
     9841 cgctgaacag cggttcattg gtctatttcc gcaagatccc ggtgggtaaa gtctacgact
     9901 atgccatcaa tcccaataaa caaggcgtgg tgattgatgt cctgatcgag cggcgtttta
     9961 ccgatctggt gaaaaaaggt agccgtttct ggaacgtttc cggcgttgat gccaacgtca
    10021 gtatcagtgg cgcgaaggtg aaactggaaa gtctggcggc actggttaac ggtgcgattg
    10081 ccttcgattc acctgaagag tcgaaacctg ccgaggcgga agataccttt ggtctgtatg
    10141 aagatctggc ccacagccag cgtggcgtaa taataaaact ggaactgccg agtggggcag
    10201 gattaaccgc cgactcgacg ccattaatgt atcagggact ggaagtcgga cagctaacta
    10261 aactggattt aaatcctggt ggtaaagtca ctggggaaat gaccgttgat cccagcgtcg
    10321 ttaccctgct tcgtgaaaat acccgcatcg aattacgtaa tccgaaatta tcccttagcg
    10381 atgccaatct cagcgccctg ctgaccggaa aaacctttga gttggtgcct ggcgatggcg
    10441 agccacgcaa agagttcgtt gttgtgccag gcgaaaaagc actgctgcat gaacctgatg
    10501 ttctgacgct gaccctgacc gcgccggaaa gttacggtat tgatgctggt cagccgctca
    10561 ttcttcacgg cgtgcaggta ggccaggtta ttgaacgtaa actcaccagc aaaggcgtta
    10621 cctttaccgt cgccattgag cctcagcatc gcgaactggt aaaaggcgat agcaaatttg
    10681 tcgtcaacag ccgtgtcgat gtgaaggtgg ggctggatgg cgttgagttt ctcggtgcca
    10741 gcgcctcaga atggatcaat ggcgggatac gtattctgcc gggcgataaa ggcgagatga
    10801 aagccagata tccactgtat gccaatctgg aaaaagcgct ggagaacagc cttagcgatt
    10861 tacccaccac aaccgtgagt ttgagtgcag agacgctgcc ggatgtgcag gcaggatcgg
    10921 tagtgctgta ccgtaaattt gaagttggtg aagttattac cgtgcgtccg cgagctaacg
    10981 cgtttgatat cgatctgcat attaagccgg agtatcgcaa ccttctgacc agcaatagcg
    11041 tgttctgggc agaaggcggg gcgaaagttc agctgaatgg tagtggcctg accgtacagg
    11101 catccccgct ctccagagca ttaaagggag ccattagctt cgataacctc agcggtgcca
    11161 gcgccagtca gcgtaaaggc gataagcgta ttctgtatgc ttccgaaaca gcagcccgtg
    11221 cggttggcgg gcagattacg cttcacgctt tcgatgccgg aaaactggcg gtcgggatgc
    11281 caattcgcta tctcggtatt gatatcgggc aaatccagac gctggatctg attaccgcgc
    11341 gtaatgaagt acaggcaaag gcggtgctct atccggaata tgtccagact tttgctcgcg
    11401 gaggtacgcg cttctcagtg gtcacaccgc aaatttcggc agctggcgtt gagcatctag
    11461 atactatcct ccagccgtat atcaacgtcg aaccaggccg tggcaatcct cgtcgcgact
    11521 ttgaattaca agaggccacc attactgatt cgcgttacct ggatggttta agcattattg
    11581 ttgaagcgcc ggaagccggt tcgttaggca tcggtacgcc tgtgctgttc cgtggtctgg
    11641 aagtaggtac ggttaccgga atgacgctgg ggacattgtc agatcgcgtg atgattgcga
    11701 tgcgcatcag taaacgctat caacacctgg tgcgtaacaa ttccgtcttc tggttggcat
    11761 cgggttacag tctggatttt ggtctgacgg gcggcgtagt gaaaaccggc acctttaacc
    11821 agtttatccg tggcggcatc gccttcgcca cgcctccggg tacgccactg gcaccgaaag
    11881 cccaggaagg caagcacttc ctgttgcagg aaagtgaacc gaaagagtgg cgtgaatggg
    11941 gaactgcgct tcccaaataa tgcccactgc tccggcgtgc ctgcgccgga gcgtttatgc
    12001 taaactgcgc gcctgttttt ttgccagtgg tatatgctcg tggcccaaca caccgtttat
    12061 ttcccggacg cctttctgac gcaaatgcgc gaagcgatgc cttcgacgct ctcttttgat
    12121 gattttcttg ccgcctgtca gcgcccgttg cgccgcagca ttcgcgttaa tacgctgaaa
    12181 atctccgttg ctgatttcct gcaattagcc gctccttatg gctggacgct tacgccaatt
    12241 ccgtggtgtg aagaaggttt ctggattgaa cgcgacaatg aagatgcatt gccattgggt
    12301 agtaccgccg agcatttaag tggcctgttt tatattcagg aagccagttc aatgttgccc
    12361 gtcgccgcct tgtttgctga cggtaatgca ccacagcggg tgatggatgt cgctgctgcg
    12421 ccaggttcca aaacgacgca aattgccgcg cggatgaata acgaaggggc aatccttgcc
    12481 aatgagtttt ccgccagtcg ggtaaaagtg ttacatgcca atatcagccg ctgtggcatc
    12541 agtaatgttg cgctcacaca ttttgatggc cgcgtgtttg gtgcggcagt gccagaaatg
    12601 ttcgatgcca ttttgctgga cgctccctgc tctggcgaag gcgtggtgcg taaagatccc
    12661 gatgcgctaa aaaactggtc accagaaagc aatcaggaaa tcgcagctac ccaacgggag
    12721 cttatcgaca gcgcctttca tgcattacgt cctggtggta cgctggttta ctcgacctgt
    12781 accttaaaca gggaagaaaa cgaagccgtt tgcctgtggc tgaaagagac ttaccctgac
    12841 gcagtagagt ttttaccact tggcgatctc ttccctggtg caaacaaagc gctgaccgaa
    12901 gaaggctttt tgcatgtttt cccacaaatt tacgactgcg aaggcttctt cgttgctcgt
    12961 ctgcgtaaaa ctcaggccat tcccgcctta cccaccccaa aatacaaagt gggcaatttc
    13021 ccgttcagcc cggtgaaaga tcgcgaagcc gcacaaattc gtcaggcggc tgcaagtgtt
    13081 ggcttaaact gggatgaaaa cctgcgcccc tggcagcgtg acaaagaact gtggttgttc
    13141 ccggtgggca ttgaagccct gatcggtaaa gtccgatttt ctcgcctggg gattaaactt
    13201 gccgaaacgc ataacaaagg ttatcgctgg cagcatgaag cggttattgc ccttgcctcc
    13261 cccgacaacg agaacgcttt tgaactgaca ccgcaagaag cggaggagtg gtatcgcggg
    13321 cgcgatgttt acccgcaagc cgcgccagtg gcggatgacg tgttggttac tttccagcat
    13381 cagccgattg gtttagccaa acggattggt tcgcgactga aaaacagcta cccgcgtgaa
    13441 ctggtgcggg acgggaaact ttttaccagt aacgcatgac agcgcacaaa aaaagcgcac
    13501 tttttgactg gcacattcgg cggcctcaac taggctgaaa aatggtgcga tcggactggt
    13561 cgtaccacaa tcggcagcta aatggagagc acgaagatga aaaccagtgt gcgcataggc
    13621 gcttttgaaa tcgacgacgg cgaattacac ggtgaatcgc cgggtgatcg aacgttaacc
    13681 attccttgta aatctgaccc cgatttatgt atgcaactgg atgcctggga tgctgaaacc
    13741 agtatccctg ccctgctaaa tggcgaacac tctgtccttt accgtacccg ttacgatcaa
    13801 caatctgatg cctggattat gcgtcttgcc tgatccaaaa agaacccgtc ggcatggcgg
    13861 gttatttgtc ctggttattc ccccgttgta aaatctctcc taaacttaac ggtacggcac
    13921 cacacttcgg ggatgaaatg ttcgcgctgg tactttttgt ttgctatctg gatggcggtt
    13981 gtgaagatat tgttgtggat gtctacaaca cggaacagca gtgtcttcat tctatgagcg
    14041 atcaacggat ccgccagggc ggttgttttc cgattgagga ttttatagat ggtttctggc
    14101 gacctgcaca ggagtacggt gatttttaat tattgcaatt gcacaagagt cagttcgccc
    14161 ccaaagacag caccggtatc aatataatgc aggttgccaa tatccacgcg atgtcgcaac
    14221 ggtgtatgac caaaccagaa atgatcagca cctgtaattc cctgcacttt ttggcgttca
    14281 cttaatcgcg agcggctcca caagacctga tgcaaatcga cgtccttttg ccattcataa
    14341 acatcatctg gataatcggc atgagcaata acatgcttgc cggtgcgact gtgtacttca
    14401 agaataaaag gcaaatgctg acatttcccc agcgccgttt tcgcttgttt ctgttgatta
    14461 tctgccagcg caataaacca gtcgccgcca ttcatcaacc ataaagacat ctgctgggat
    14521 gccagcgcat ccatcgccat ctgttcatga ttgcctctta ccgcacgaac ccaatgttgt
    14581 tccagtaact gcagacaacg taaactttgc ggcccacgat cgataacgtc tcctactgag
    14641 ataagtaaat ctcgccacgg atcaaaacga caatgccata atttgcggcg caactgctca
    14701 agacaaccgt gtatatcgcc agaaagccag atatgtcgcc attgatgacc cgcaattctc
    14761 tgataaacgg gcgcaggctg tttcatcaat attttcctcc cgcgctaaag atcacataat
    14821 cttaacaaga atgttaaaaa acgctggact cagacagtag agtgtgtgtt atggttgact
    14881 ataaagtcag cgaaggaaat gcttctggct tttaacagat aaaaagagac cgaacacgat
    14941 tcctgtattc ggtccaggga aatggctctt gggagagagc cgtgcgctaa aagttggcat
    15001 taatgcaggc ttagttgcct tgccctttaa gaatagatga cgacgccagg ttttccagtt
    15061 tgcgtgcaaa atggtcaata aaaagcgcgg tggtcatcag cttaaatgtt aaaaaccgcc
    15121 cgttctggtg aaagaactga ggcggttttt ttattggaaa tcaaaaggct attttaggta
    15181 attaacagag tttttcagct cgttctataa acggtgccag actcattttt tcgccgggat
    15241 tgttaggatc atcaatctga atcaccgaaa tgggttgggc tttagtcttc ccactggcaa
    15301 cttccttttg tgcgatatcg tttaaagggt actgcacgag ggtacttggg ttgatgacat
    15361 acaaagcatt acccggtcgg caagtcagca tcacctcttc gcgattaaac gcccatttgt
    15421 ctttacccac ttcaaaacgg ctgacggtaa tcacctgcgg tgcagccagc gccgctgcag
    15481 aactggtgag taacagaaac gccagaatac tttttttcat cattttcgta atccatcaaa
    15541 aaggttcgag agtcgcaagc aggctgatga tcaccagcac taccgcacct atcgcccatt
    15601 cgagtttagt catccacaca aaccagggtg ccgcacgatc ttcatcctgc ctcatgcgcg
    15661 gtacaagaac ataacgatta gccagcgcaa tgaccaccat aatcatcacc aggatggctt
    15721 tgagcaataa gagttggccc cagtaagtcg taagcgtggg gggaaatccg gtaatcaaca
    15781 atgcattaag cacgccgctt gccagtacgc cgatcaccgc aaaatgcccg caccaggaaa
    15841 aacgcatcag cgcctgaatg gcctggtgtc gccaacggcc tttgatgagt tgcatacacc
    15901 agagcaccgg cagtaatccc ccaaaccacg ccgccgcaca aatcaggtga atcgcgtgat
    15961 tagtctgatg gattttcgcc gttacccctt cattcagtgt cgcatgcccg attcccgcca
    16021 gcagaataaa ttgcgcggta gtgagcataa acagcaaacg tggcatattc cgcggttgca
    16081 taagggcgac gatcaacgtt acgagggcga gaacaatttg ccataaccag acgccaccaa
    16141 actgcgtttg caaaacagcc tgccaaatgt ttggcgaaaa tacatctgtc catcccgttc
    16201 ccatcaaccc gccctgaacg gcgagcattg cagtggcact gatcaaactc cagacggcag
    16261 cgtgctgttg taaacgcaga aaacgcttcg ccagcaagcg acgaatcgtc aagggggcca
    16321 gccaggcacc gtacattgcg aagccaaaaa ccagcatcag cgaggtgaaa tggataaatc
    16381 gcagcgcgat ccaggtaaac gccagcatga tttatttcac gctaaaggtg tagtgtcctt
    16441 tcgttttatg cccgtccacc gaaacaacat gccagtcaac ggtgtaggtt ccgggtttca
    16501 gcgaatcggc cagtggaaca atcagttgct tttggtcctg ctcatttcgc ttcgccggta
    16561 atgttttaat attttcgttt tttggccccg tgatttttgc accactgaat cctgtttcaa
    16621 caccttccga gaagtttaaa gtgattgcct gcggtgcagc tgtcacttgc gcgtttgccg
    16681 caggatactg atgcgttaaa tgcgcatgtg cccagacgga aggtgtcacc aacgaagtgg
    16741 tcaggattgc cagcgcgtag cgaagggagc gtgcagttga agccatatta tctattcctt
    16801 tttgtaataa cttttttaca gagcataacc ttgtctaatg tctgagtcga ggatcatcaa
    16861 ttccggcttg ccatccaggc tcactcttag taacttttgc ccgcgaatga tgaggagatt
    16921 aagaatgctg aagaatctgg caaaactgga tcaaacagaa atggataaag tgaatgtcga
    16981 tttggcggcg gccggggtgg catttaaaga acgctacaat atgccggtga tcgctgaagc
    17041 ggttgaacgt gaacagcctg aacatttgcg cagctggttt cgcgagcggc ttattgccca
    17101 ccgtttggct tcggtcaatc tgtcacgttt accttacgag cccaaactta aataaaactt
    17161 atacagagtt acactttctt acataacgcc tgctaaatta tgagtatttt ctaaaccgca
    17221 cccataattt gtagtcattt tgaaaaggaa gtcattatgt cgttctggaa agttgcagca
    17281 gcgcaatatg agcctcgtaa aacctcactc accgaacagg tagctcatca tctggagttt
    17341 gtgagggccg ccgccaggca acaatgtcag ctgttagttt ttccatcgct ttctttactg
    17401 gggtgtgatt attcgcgacg tgcccttcct gccccacccg atctctcact gttagacccg
    17461 ctttgttatg ccgcaacgac ctggcgaatg accatcattg ccggccttcc cgttgaatat
    17521 aacgatcgct ttattcgtgg aattgcggtg ttcgcaccgt ggcgaaaaac gccggggatc
    17581 taccatcaga gtcatggtgc atgtctgggc cgtcgttcca gaaccattac ggtggtcgat
    17641 gaacaaccgc aaggtatgga tatggacccg acctgttcac tgtttacgac agggcaatgt
    17701 ctgggggaac ccgacctact ggcatctacc cgccgcttac aatttttttc acatcagtac
    17761 tccattgccg tgctgatggc aaatgcccgt ggtaacagtg cactatggga tgaacatggt
    17821 cgtctcatcg ttcgcgccga tcgtggttca ttattgttag tcggtcagcg ttcatcacag
    17881 ggttggcaag gcgatatcat tccattacgc taggcttttt cggcctggag catgccatgt
    17941 tgcgcattat cgatacagaa acctgcggtt tgcagggagg gatcgttgag attgcctctg
    18001 ttgatgtcat tgacggaaaa atcgtcaacc ccatgagcca cctggtgcgc cccgatcgtc
    18061 ctattagtcc acaagcaatg gcgattcatc gcatcaccga agccatggtc gccgataaac
    18121 cgtggattga agatgtgatc ccacactatt acggtagtga atggtatgtc gcgcataacg
    18181 ccagctttga ccgccgcgta ctgcctgaga tgcccggtga gtggatttgc actatgaaac
    18241 tggcccgtcg tttgtggcca gggatcaagt acagcaatat ggcgttatat aaaacacgga
    18301 agctcaatgt acagacgccg ccgggcctgc atcatcaccg cgcgttgtat gactgttata
    18361 tcaccgccgc gttgcttatc gatattatga acacctccgg ctggacggca gaacagatgg
    18421 ccgatatcac cggacgtccg tcgttgatga cgaccttcac ctttggcaaa taccgtggca
    18481 aagcggtttc cgacgttgcc gaacgcgatc cgggctatct gcgctggtta tttaataacc
    18541 tggacagcat gagcccggag ctgcgtttaa cactgaaaca ttatctggaa aatacttaat
    18601 ccgcaggcgt agcgggtaat gttccctgcg ccagcgcgac cagaaaagca tattccatcg
    18661 ctacgccttc gtacgattta aagcgaccag atttaccgcc atggcctgag tccatgtcgg
    18721 tacagagcaa taaaagatgg tcatcggttt tcagctcgcg caatttagcg acccattttg
    18781 ccggttccca atattgcacc tgagaatcgt gcaaaccggt cgttaccagt aaatgcggat
    18841 aagcctgtgc ggtgacgttg tcatacgggc tgtagctttt catgtactcg taatattgcg
    18901 gatcctgcgg gttaccccac tcttcaaact caccagtggt aagaggaatt gattcatcaa
    18961 gcatcgttgt tacaacatca acaaacggta cctgggcgat aacgccgtgg aataattccg
    19021 ggcgttgatt aattgcaacg cccatcaaca tgccccccgc actcccgccc atcgcataac
    19081 aaagcgaagg agagccatag cccagtttta acaatgcatc gcaggcatca agataatcat
    19141 taaacgtatt tttcttcttc agaaattttc cgtcttcgta ccattgttgc cccagctcac
    19201 caccgccgcg aacatggaca atggcgtaga caaagccacg atctaacaaa ctcaagcggc
    19261 taaaactgaa atcggcatca atacttgcgc cgtaagaacc atagccatac accagcaacg
    19321 ggttgtgtcc tttgcgaaaa tgtttgcgat ggtagaccaa cgaaaccgga acttcgacgc
    19381 catcacgggc gactatccac aggtgttcac tgcggtaatt cgccgcatca aaaccgggca
    19441 cttctgtttg ttttaataca cgacgctcac cggtatccat atccagttca aacaaagtgt
    19501 ctggtgtggt catggaagaa taaccataac gcaatcgcgc ggtttcaggt tctggattgt
    19561 aggcaatcca ggtcacatag gctggatcat caaaggcaat accaatgact tcccgggtct
    19621 tgcggttaat ttggcgcaaa ctggttaacc cgcgctgacg ctcttcaacc accagccagt
    19681 cggtaaacag cgtaaaccct tccagcatga tgttttcgcg tggcggaatt aactcttccc
    19741 actgttgctc atcacgcata cgggtacggt ataagccaaa gtttttgccg tggcggttgg
    19801 aacgcagata aaaacgatgc tggtagtgat caaggctgta ttcgtgatct ttgcggcgcg
    19861 gcagaaaaac aaacggctcg gcatcggcca tttccgcgtc cagcaggcga acttcactgg
    19921 tggtggcgct ggccaaatga ataactacat agtgcttcga ggtcgtttta tgcaggctga
    19981 cgtaataggt atcgtctttt tcttcgtaaa tcagtttatc ttgcgatgct ggcgtaccga
    20041 tggcgtgacg ccagacctga taaggcagca gcgtcaccgg atgcttgcga acatagtaga
    20101 aagtccagga gtcatttgcc cagacaaagc tgggttcaac gttatccagc agttccgggt
    20161 accagttacc tgtttccaga ttacgaaaac gaatgccgta ctggcgtcgg gaaagaaaat
    20221 cttctgccag cgccatgatg gtgttatcgg gcgtaatcgc cattccgccc atcgaataaa
    20281 actcactatg agctgcgcgc ttattggcat cgagcaatgt ttcccactca tcccactctt
    20341 cactgaatgc cgattgacgc tggtagatag catattcaca gcctggttca taaatatgcc
    20401 gatagcggta gccatttttg atgtagggcg cagaaacttc tcgttgcgga atgcggtcga
    20461 tgatttcctt taagatgcga tcctgcaagg cttgttgtga ggccatcacc cgatgaccgt
    20521 aactattttc ttgttgcagg tagtccagga cttctggctg agaacgcgta tcgtcccgca
    20581 gccagtagta attatcgatg cgcgtatcgc catgaagcgt catggcgtgg ggaatgcggg
    20641 cggcttttgg tagcatgtta ttgttctttc tggttgaaac atcttataag ggtggcaaaa
    20701 ctcaccgggg atgcaagcga aacaggggag tcattgctta gatgatgaca ggtaatggcg
    20761 cggatatcga atgttatgca gacagagaaa atcagcctgt tcaaatggct gtgcgattct
    20821 ggatagcccg aaatagtcaa cttcaggcta tccagagagc ggaattattc cgccaaagtg
    20881 cgtttttgct gttcgagatc gcgttcaatg ccgtcacgaa catcctgggg gattttcagc
    20941 gcgtcaccca gtgcattcag gtaactgcgt tccataaaat ggtcaatatc aatagccgcg
    21001 caactcagga aatagatttc cagtgcctct tcttcattgc ggaccccggt agccaggcgt
    21061 tgtggatcca gcggttgttc gattgcctgc tcaatgagta cacgcccctg ctcttccacg
    21121 ccggcttcac gcaattgctg gtcgattgcc gcacgttctt tggcatcaat atgaccatca
    21181 cttttagcgg caaagaccag cgcaaggatc aaacgttctg tacgctcatc cagcggcgta
    21241 ctttgcgcgc caaactgcgg ttcgtcctga tgcgccgcgc gaattttatc tttgtattta
    21301 ttccacagca ccgtacccgc taccgctccg ccgccaacca gtaacgcgtt ggtgccatat
    21361 ttcgtaagaa gtttacgtgc tgatttattt gcgaccagca gcccagccag tccgcctaat
    21421 gcgcctggca ctaacagttt gcccaatccc tgatccgcag acgaggaggt agaagaactg
    21481 ctttgcccaa gcagggattg cagttgattt aaccagttag ccatatttgc tcctcaataa
    21541 ccatttttag tgcctgtagc atagcggatg aggatgtcag aaaatgtata tcgagacaaa
    21601 agatgcgcaa ataagcgggg cggtaggccg ttattcaaag aaattcgcgc caggtaaggt
    21661 atttactgta tatcaggtga taagggtatt ttaggtgaaa gtagtttgtc gttttgagtg
    21721 gcattgtttg atgctgattg cccgatgcga cgctgttgcg tcttctcagg cctacgattg
    21781 tggtgcgttc tggggcggac aaggcactcg cgccgcatcc gccaacggtc aatcgaatta
    21841 tcagaagcca ttaacgccgc tgatattccg ccattcccgc tttgcagtcc acgctgacct
    21901 ggtaatgaat atcggcactt ttaccacgca cggttagcgg tactgaccat ttatcatctt
    21961 taccctgaat gtcctgcaaa ctgacccaag ccacgggatc ggcctgaccg acaatttttt
    22021 gatcatctgc ccaacgcgcc acgcgatttt gttgataatc acgttttacg ctcgcggcaa
    22081 ttccggcagc atccagacct tcacactttg ggaaagtgac cgacttgctg gtttcattat
    22141 tggcagcgaa aactgatgcg caggcagaaa ccaacaacag ccctaaaaac gcccctcttt
    22201 ttttcatgtt tttctccata gcacaatgat tcaggagaaa gcatggtaca aattgtcagg
    22261 agcgcaagtt gcttcaggcc gcgtgtgagg catcttcctc ttcggattca gcaccgacga
    22321 cctgtgggga cggtaatttg ccggttttca gaatggtgct cagcacatct ttttgttctg
    22381 ccagccatag cgaaagggct tcacgttgct cgtcttccat ttgcactgga gaatttgtca
    22441 gccaggtgtc aagcagatcc gccgtatcaa gcattttatc ataagcatcg gcttcctttt
    22501 tgctggtaaa cgacattttc tcttcgccct cacgaatgac tacgtattta acttcaaccg
    22561 ccatttgcag cctctcataa taactgtgat tttatacagt atatttcttt tcggttgaga
    22621 aatcaacatc agcaataaag acacacgcaa acgttttcgt ttatactgcg cgcggaatta
    22681 atcaggggat attcgttatg acgttattag gcactgcgct gcgtccggca gcaactcgcg
    22741 tgatgttatt aggctccggt gaactgggta aagaagtggc aatcgagtgt cagcgtctcg
    22801 gcgtagaggt gattgccgtc gatcgctatg ccgacgcacc agccatgcat gtcgcgcatc
    22861 gctcccatgt cattaatatg cttgatggtg atgcattacg ccgtgtggtt gaactggaaa
    22921 aaccacatta tatcgtgccg gagatcgaag ctattgccac cgatatgctg atccaacttg
    22981 aagaggaagg actgaatgtt gtcccctgcg ctcgcgcaac gaaattaacg atgaatcgcg
    23041 agggtatccg tcgcctggcg gcagaacagc tgcagctgcc cacttccact tatcgttttg
    23101 ccgatagcga aagccttttc cgcgaggcgg ttgccgccat tggctatccc tgcattgtaa
    23161 aaccggtgat gagctcttcc ggcaaggggc agacgtttat tcgttctgca gagcaacttg
    23221 ctcaggcatg ggaatacgct cagcaaggcg gtcgcgccgg agcgggccgc gtaattgttg
    23281 aaggcgtcgt taagtttgac ttcgaaatta ccctgctaac cgtcagcgcg gtggatggcg
    23341 tccatttctg tgcaccagta ggtcatcgcc aggaagatgg cgactaccgt gaatcctggc
    23401 aaccacagca aatgagcccg cttgcccttg aacgtgcgca ggagattgcc cgtaaagtgg
    23461 tgctggcact gggcggttat ggtctgtttg gtgtcgagct gtttgtctgt ggtgatgagg
    23521 tgattttcag tgaggtctcc cctcgtccac atgataccgg gatggtgacg ttaatttctc
    23581 aagatctctc agagtttgcc ctgcatgtac gtgccttcct cggacttcct gttggcggga
    23641 tccgtcagta tggtcctgca gcttctgccg ttattctgcc acaactgacc agtcagaatg
    23701 tcacgtttga taatgtgcag aatgccgtag gcgcagattt gcagattcgt ttatttggta
    23761 agccggaaat tgatggcagc cgtcgtctgg gtgttgcgct ggcgaccgct gagagtgttg
    23821 ttgacgccat tgaacgcgcg aagcacgccg ccggacaggt aaaagtacag ggttaaaccc
    23881 ggcaaaaaaa cgctacaaaa atgcccgatc gtagatcggg cgttttgact tttacagctt
    23941 agcgccttct acagcttcac gcgccagctt agtaatgcgg tcgtaatcgc ccgcttccag
    24001 cgcatctgcc ggaaccagcc aggaaccacc gatgcacagc acgcttttca gcgccaggta
    24061 gtcacggtag ttagccggag aaataccacc cgtcgggcag aaacggacct gggagaacgg
    24121 acccgcgatc gcctgcaggg ctttcacgcc gccgttagct tcagccggga agaatttgaa
    24181 ctctttcaaa ccgtagtcca tacccagcat cagttcggaa acagtgctga tccccggaat
    24241 tagaggaata gtcccttcgg tagcagcttt cagcagcggc tcggtcagac ccgggctaat
    24301 tgcgaactgt gcacccgctt cagtgacttc tgccagctgc tgtggattca gcaccgtacc
    24361 ggcacccaca atcgcttcag gcacttcttt ggcgatagca cggatagcgt caactgcaca
    24421 ctcggtacgc agagtcactt ccagaacgcg caccccacca gcaaccaacg cttttgccat
    24481 cggcactgcg tgttccagtt tttttaccac gataaccggt acaaccgggc cggtggtcag
    24541 gattgattct gcacttgttt tccagttttt catcagagtt ttctctcgcc tgattacaaa
    24601 tttgtcgtct taaaaagtga tacaggttgc gccctgttcg gcaccggaca gtttttcacg
    24661 caaggcgctg aataattcac gtcctgtccc cacgcgtgac gcgctcaggt caggaatgtg
    24721 cggttcgcga gcagccagtt ccgcttcgtc taccagcagc gtcagttcgc ctgtctgtcc
    24781 attcacacga atgatgtccc cgtcgcgcac ttttgccagc agcccgccat cgtaggcttc
    24841 tggtgttacg tggatagctg acggcacttt acctgaagcg ccggagagtc gtccatcggt
    24901 aactaacgca attttgaaac accggtccaa taatacacca agtggcggca tgagtttatg
    24961 taattctggc attccgttcg cttttggccc ctgatgacgg acaacaacga cacaatcgcg
    25021 gtccagcaaa cccgcttcaa aggccggcat aacgtcatgc tggctttcaa aaacaaccgc
    25081 tggcgcttca atcacctggt tctcaaccgg cacggcagag gttttcataa ccgcacggcc
    25141 caggttaccg cttaacactt ttgtcccacc atgatgagag aaaggttgtt cgaaggaagc
    25201 gatcacattg ctgtcgagtg atttttccgc cccttcccgc cagtccagtt caccattatt
    25261 cagccatggt tcaagggtat aacgagacag accaaagcct gccaccgtat tgacatcttc
    25321 atgcagcagg cctgctttga gcagttcacg caccagaacc ggtacgccac ctgccgcctg
    25381 gaagtggtta atatcggccg gaccgttcgg gtagagacgt gccatcagcg gtacaacatc
    25441 agaaaggtca gagaagtcat cccagttaat ctgaataccg gccgcgcgcg ccatcgccac
    25501 caggtgcatg gtgtggttag tggaaccacc ggtcgccagc agtgcaacga taccgttcac
    25561 caccactttc tcatcgatca tcttaccgat cggcatccat tcattaccat taccggtcat
    25621 gcgtgtaacc tgacgcgcag cggcagcggt caaagcatcg cgcagcggag aatccggatg
    25681 aacaaaagaa gagcctggca actgcatccc cataaattcc accaccatct ggttggtgtt
    25741 ggcagtaccg tagaaagtac atgttcccgg cgcatggtaa gacgcggctt ctgactccag
    25801 taaggccatg cggtccactt taccttcggc ataaagctgg cgaatacgca ctttttcttt
    25861 atttggcaaa ccgcttgcca tcggtccaga cggcacaaac accgcaggca aatgaccaaa
    25921 cgacagggct gccatcgtca gacccgggac aatcttgtcg cacacaccga ggaacagagc
    25981 accatcaaac atgttatggg acagcccgac cgccgcagac atcgctatca cttcgcggct
    26041 tagcagcgac aattccattc catcctgccc ctgggtgaca ccatcacaca tcgccggaac
    26101 accgcccgca acctgaccaa ccgcattcgc ttcatgcagg gctttacgaa tgatttctgg
    26161 atagtgttca taaggctggt gagcggagag catgtcgtta taggaggtga tgatggcgat
    26221 attgttacgc aacatgcttt tcaaagaggc tttgtcttct ggctggcagg cagcgaaacc
    26281 gtgtgccagg ttaccgcatg ccaactgcga acgatgaacg gtcgacgttt tcgcttgttc
    26341 tatccgggcg agataagcag agcgagtctc gcgcgaacgt tcaatgattc gatttgttac
    26401 gcgtaacaat tgtggattca taaaggctcc tgaaattgag ttgtcagagc aggatgattc
    26461 acaacgcgtt tcattcagag gatttatgac tgaaacgcct gtaaccggag ctcatagggc
    26521 aaaaacgttt cagtcagtgt aataaaaaaa gcctcgtggg tgaatccgca cgaggcctga
    26581 aagtgtaaaa attgttctac aatctgtgca agatcatgtt accggtaaaa taaccataaa
    26641 ggataagcgc agatattact caaactcatt ccaggaacga ccatcacggg taatcatcgc
    26701 caccgaggca acgggtcccc aggttccggc ctgatacggt ttcggcgcat cattgtccat
    26761 cgcccacgcc tcagtaatgg agtctaccca tttccaggct tcttccactt cgtcgcgacg
    26821 aacaaacagt gcctgaatac cacgcatggt ttccagcagc aaacgttcat aggcatccgc
    26881 cagatgcgtc tgattaaagg tttctgaata gctcagatcc agcttggtga tttgcaggtt
    26941 atgtttgtgg tcaaggccag gaactttatt cagtacctgg atatccacgc cttcatcagg
    27001 ttgcagacgg atagtcagtt tattctgcgg cagatcctgc cacgattctt taaacagatt
    27061 cagttcaggt gttttgaaat agaccacgac ttcagaacat ttggtcggca gacgtttacc
    27121 agtacgcagg tagaatggca caccggccca gcgccagtta tcaatgtcga cgcggatcgc
    27181 tacgaaggtt tctgtattgc tgctcttgtt cgcgccctct tcttccagat atcccggcac
    27241 ttttttgccc tgggcgaagc ccgcagtata ttgcccgcgt acggtttttt cgcgtacgtt
    27301 ggagcggtcg atgcggcgca gagacttcag tactttcact ttttcatcgc ggatgctgtc
    27361 tgcgctcagg tcagacggcg gagacatcgc aatcatgcag agaatttgca gcaggtggtt
    27421 ttggatcata tcgcgcatct gaccggcttt atcaaaatag ccccagcgcc cttcgatccc
    27481 cacttcttct gccacggtaa tctcaacatg atcaatggtg cgattgtccc agttattcac
    27541 aaacagggag ttagcaaaac gcagcgccaa caggttcagc accgtttctt taccaagata
    27601 gtggtcgata cggtaaacct ggcactcctc gaagtattcg ccaacctgat cattgatttc
    27661 ctgcgaggtc gccagcgacg tccccagcgg tttctccatg actacgcgtg ccggtttagc
    27721 attcagttta gcctcgccaa gccctttgca aattgcgcca aaagtgctgg gcggcatggc
    27781 aaagtagtta atggtgatac gatttttttg atccagcatc gcgccgagac ggctgaatgc
    27841 agcagtgtca ttgacatcga gattacaaaa atccagacgt gcactcaggg tgtcccataa
    27901 accttcatca atggtttctt tcatgaaagt ttcgagcgcc tcgcggacaa ctttggtata
    27961 tgccgcttta tcccagtcag cacgccctac gccgataatc cgggtgtccg ggttgagctg
    28021 accggctttt tccagttgat acagggaagg cagcaattta cgacgcgcaa ggtcgccttt
    28081 cgcgccgaaa atgaccaggt cacaggcctg ggctgtttgc gttaccgcca tgtcattctc
    28141 cttaagttaa ctaacccggt acttaagcca gggtatactt gtaattttct tacggtgcac
    28201 tgtactgctt ttacgagctt gcgaaaactg taaacgctta tccacccgtg cgattatggt
    28261 aaaagcgcgc aaagtgcggc aaaactgata aaaaaatcat cgttttccat tgggtaaaaa
    28321 tctgacactg atcatgttat gaaaaaaaat aacaactttt ttatctgctt ttgtcattaa
    28381 cacagcacac aggcgtaata tctgacaaaa ctacatttcg atttctttca gtgcggaaat
    28441 cgtcattacc cgtgagtttc tttacatcat gaatatgctg gaaaaaatcc agtctcagct
    28501 ggaacatttg agcaaatcag agcgcaaagt tgccgaggtc attctggctt cgcccgataa
    28561 cgcgatccat tcgagtattg ctgctctggc gctggaagcc aatgttagcg aaccgacggt
    28621 gaatcgtttc tgtcgcagca tggacacgcg cggttttcct gattttaaac ttcatctggc
    28681 acaaagtctg gcgaatggca ctccctatgt taatcgcaat gtcaatgaag atgacagcgt
    28741 tgaatcatac acagggaaaa tatttgagtc cgcaatggca acgcttgatc atgtccgtca
    28801 ttcactggat aaatctgcca tcaaccgcgc cgtcgacttg ctcactcagg caaaaaaaat
    28861 cgcctttttc ggattaggct cttcagccgc cgttgcccac gatgcgatga ataagttctt
    28921 tcgttttaat gttccggtgg tgtactccga tgatatcgtg ctgcaacgca tgagttgtat
    28981 gaattgtagc gacggagacg tggtggtgct gatttctcac actggaagaa caaaaaatct
    29041 ggtcgagctg gcgcagctgg ctcgcgaaaa cgacgccatg gtgattgccc tcacctctgc
    29101 gggtaccccg ctcgcccggg aagcaacgct ggcaattacc ctcgacgtac cggaagatac
    29161 tgacatttat atgcccatgg tttctcgact tgcacagctg accgtgatag atgtgctggc
    29221 gacaggattt actttgcgac gcggtgcaaa attcagagat aacttgaagc gggtcaagga
    29281 agcgctgaag gaatcgcgtt ttgataagca gttacttaat ttaagtgacg atcgctaaaa
    29341 acgactgtca ctgtcctaat cttatacgac atccgaatga gattaattta tcgccatcgc
    29401 ggcgttattt cattcggatt tcatgttcaa gcaacacctg gttgtttcag tcaacggagt
    29461 attacatgtc cagaaggctt cgcagaacaa aaatcgttac cacgttaggc ccagcaacag
    29521 atcgcgataa taatcttgaa aaagttatcg cggcgggtgc caacgttgta cgtatgaact
    29581 tttctcacgg ctcgcctgaa gatcacaaaa tgcgcgcgga taaagttcgt gagattgccg
    29641 caaaactggg gcgtcatgtg gctattctgg gtgacctcca ggggcccaaa atccgtgtat
    29701 ccacctttaa agaaggcaaa gttttcctca atattgggga taaattcctg ctcgacgcca
    29761 acctgggtaa aggtgaaggc gacaaagaaa aagtcggtat cgactacaaa ggcctgcctg
    29821 ctgacgttgt gcctggcgac atcctgctgc tggacgatgg tcgcgtccag ttaaaagtac
    29881 tggaagttca gggcatgaaa gtgttcaccg aagtcaccgt cggtggtccc ctctccaaca
    29941 ataaaggtat caacaaactt ggcggcggtt tgtcggctga agcgctgacc gaaaaagaca
    30001 aagcagacat taagactgcg gcgttgattg gcgtagatta cctggctgtc tccttcccac
    30061 gctgtggcga agatctgaac tatgcccgtc gcctggcacg cgatgcagga tgtgatgcga
    30121 aaattgttgc caaggttgaa cgtgcggaag ccgtttgcag ccaggatgca atggatgaca
    30181 tcatcctcgc ctctgacgtg gtaatggttg cacgtggcga cctcggtgtg gaaattggcg
    30241 acccggaact ggtcggcatt cagaaagcgt tgatccgtcg tgcgcgtcag ctaaaccgag
    30301 cggtaatcac ggcgacccag atgatggagt caatgattac taacccgatg ccgacgcgtg
    30361 cagaagtcat ggacgtagca aacgccgttc tggatggtac tgacgctgtg atgctgtctg
    30421 cagaaactgc cgctgggcag tatccgtcag aaactgttgc agccatggcg cgcgtttgcc
    30481 tgggtgcgga aaaaatcccg agcatcaacg tttctaaaca ccgtctggac gttcagttcg
    30541 acaatgtgga agaagctatt gccatgtcag caatgtacgc agctaaccac ctgaaaggcg
    30601 ttacggcgat cattaccatg accgaatcgg gtcgtaccgc gctgatgacc tcccgtatca
    30661 gctctggtct accaattttc gccatgtcgc gccatgaacg tacgctgaac ctgactgctc
    30721 tctatcgtgg cgttacgccg gtgcactttg atagcgctaa tgacggcgta gcagctgcca
    30781 gcgaagcggt taatctgctg cgcgataaag gttacttgat gtctggtgac ctggtgattg
    30841 tcacccaggg cgacgtgatg agtaccgtgg gttctactaa taccacgcgt attttaacgg
    30901 tagagtaagt acgttgccgg atgcggcgaa aacgccacat ccggcctaca gttcaatgat
    30961 agttcaacag atttcgaata ttctgaagca aacttgaact tatcatcagg cgaaggcctc
    31021 tcctcgcgag aggctttttt atttgatggg ataaagatct ttgcgcttat acggctggat
    31081 ttcgcccggt ttgcgagttt tcagcaattt tagtatccag gtgtattgtt ctggtcgcgg
    31141 accaacaaaa atctcgactt cttcattcat ccgccgcgca atcgtatgat catccgcctc
    31201 taacagatca tccatcggtg ggcgcacctg aatcgtcaga cgatgcgtct tgtcatcata
    31261 aatcggaaac agcggtacaa cgcgcgcacg gcacactttc atcaaacgac caatcgcggg
    31321 caacgtcgct ttataggtgg caaagaaatc cacaaattcg ctgtgctctg ggccatgatc
    31381 ctgatcgggt aaataatatc cccagtaccc ctgacgtacc gactggatga atggtttaat
    31441 accgtcattt ctcgcatgca gacgaccgcc aaagcgacga cgcaccgtgt tccagacata
    31501 atcaaaaacc gggttgccct gattatggaa catcgctgcc attttctgcc cttgcgaggc
    31561 catcagcatg gcaggaatat cgacggccca accgtgcggc accagaaaga taactttctc
    31621 gttattacgc cgcatctctt cgatgatctc cagcccttgc cagtcaacgc gcggctgaat
    31681 tttctccggc ccgcgtattg ccaactcagc catcattgcc atcgcttgcg gcgcggtggc
    31741 aaacatctca tcaacaatcg cttcgcgttc agcttcacta cgttctggaa agcagagcga
    31801 cagattgatt aacgcacgac ggcgtgagct ttttcccagt cgtccggcaa aacgtcccag
    31861 ccgtgccaga atgggatcac ggaactttgg cggcgttaaa gcgatacccg ccatcgctgc
    31921 tacgcccagc catgctcccc agtagcgcgg gtggcgaaag gatttatcaa actcaggaat
    31981 gtattcgcta ttattttttt tcgtttccat gcttttccag tttcggataa ggcaaaaatc
    32041 aatctggtga tagtgtagcg gcgcaacttg ccccgcacca aataaaaaaa gccggtactg
    32101 actgcgtacc ggctgcgaat ggatgttaat taatcaaacc gtagctgcgg cacaatctct
    32161 ttggcctgcg ccaggaattc gcgacgatcg gagccggtca gcccttcggt acgcggcagt
    32221 tttgccgtca gcgggtttac ggcctgctgg tttatccata cttcatagtg cagatgcggc
    32281 ccggttgaac gtccggtatt accggaaagc gcgatacggt cgccacgttt caccttctgt
    32341 cccggtttca ccagaatctt gcgcaagtgc atataacgcg tggtgtagct gcgaccatga
    32401 cgaatagcca cataataacc tgctgcgcca ctgcgtttgg caaccaccac ttcaccgtca
    32461 cccactgaaa gcactggcgt accctgcggc atggcgaaat caacacctct gtgtggtgca
    32521 acgcgacctg tcaccggatt agtacgacgc gggttaaagt tagacgagat acggaactgt
    32581 ttcgccgtcg ggaatcgcat gaatcctttc gccagaccag taccgttacg atcgtagaat
    32641 ttgccatctt cagcgcggat agcgtaataa tctttacctt ctgaacgcaa acgtacgccc
    32701 agcagctggc tttgctcacg tttaccatca agcatttctc gagacattaa taccgcaaat
    32761 tcatcgcctt ttttcagttt gcggaaatcc atttgccact gcatggcttt aatcactgcg
    32821 ctcacttcgg cgctggttaa accggcgttt ctggcgctgg caacaaagct ccccccgacg
    32881 gtacctttca gcagattgtt aacccactct ccttgctgca tttcgctggt cattttaaaa
    32941 ccgttagcgg cagtacggtc ataggttcgg gtttcacgac gagacacttc ccaggtgagg
    33001 cgctgcagtt cgccgtccgc ggttaatgtc caggagagtt gttgaccgat tttcaggtta
    33061 cgcaattctt tgtcggccgc agccagttgg gtgatatcac ccatatcaat accatactga
    33121 ttgagaatgc tgcttagcgt atcgccagtg gaaacaacat attcatgcac gcccgcttca
    33181 ccggcaattt tgtcatccag ttcgtcctgg ggaatggctt catcttcttg tgcagcttga
    33241 tcaatcggtt cactggcttc aggtaagagc gaacgaattt cgttctgttc cagctcaatg
    33301 gttttgacaa ttggcgtggc atcgcggtga taaacatagg gccgccagac agcgacggcc
    33361 agagtaagaa cggtgagcga ccccaacata acgcggtgtg gtcgcggtaa attattaaac
    33421 gccagggcga cagagcgggc tatctgttgc acgtaatcac ttcctcatta atctcctttc
    33481 aggcagctcg catactggtt ggctaattga ctcaggaatt ctgaatagct tgttttaccc
    33541 agtttgatat tcgtccccag gggatccaac gttcccatac gaacggatgt ccctcgtgcg
    33601 acgctctcaa cgaccgctgg cctgaactgt ggctcagcaa aaacgcaggt tgctttttgc
    33661 tcaaccaact gtgttcttat ttcatgtaaa cgctgcgcgc caggttgaat ctcagggtta
    33721 acggtaaaat gaccaagcgg tgtcagtccg aactgttttt cgaaatagcc gtaagcatcg
    33781 tgaaaaacga aataaccttt ccccttgagc ggcgcgagct cgttaccaac ctgcgtttcg
    33841 gttgaggcta attgtgcctc aaaatccttc aggttggcgt caagtttggc tcgactttgc
    33901 ggcataagtt ccactaattt tccatggatt gcaaccgctg tagcccgcgc tatctctggg
    33961 gaaagccaaa gatgcatgtt gaaatcgccg tgatggtgat cttcgtcact tttttccgcg
    34021 tggtcgtgat catcatcatc gccgtgaata cttttcatca gtagcggttt cacatcttca
    34081 agctgcgcaa tcgttacctg cttcgcttct ggtaatttgc ttaccggttt ttgcataaac
    34141 gcttccatct ccgggccaac ccaaacgact aagtccgcgt tctgtaagcg ttttacatcc
    34201 gatggacgca gtgaataatc atgttctgaa gcgccgtcag gaagtaaaac ctccgtttct
    34261 gttaccccat cagcaatggc agaagcgatg aacccaacgg gtttaagcga agcgacaacg
    34321 gcggcatctg cggcctgtgt tgcacctccc cagagagcgg cggataatgc tgcgaaaaga
    34381 agcgtttttt tatgtaacat aatgcgacca atcatcgtaa tgaatatgag aagtgtgata
    34441 ttataacatt tcatgactac tgcaagacta aaattaacat gacaagtctg gtttccctgg
    34501 aaaatgtctc ggtttctttt ggccaacgcc gcgtcctctc tgatgtgtcg ctggaactta
    34561 aacctggaaa aattttgact ttacttgggc caaatggcgc aggtaagtcg acactggtac
    34621 gggtagtgct cgggctggta acacccgatg aaggggttat caagcgcaac gcaaaactgc
    34681 gcatcggcta tgtaccgcag aagctgtatc tcgacaccac gttgccactg accgtaaacc
    34741 gttttttacg cttacgccct ggcacacata aagaagatat tttgcctgca ctgaaacgtg
    34801 tccaggccgg gcacctgatt aacgcaccga tgcaaaagct ctctggtggc gaaacgcagc
    34861 gtgtactgtt agcgcgagca ttgttaaatc gaccgcaatt attagtgctg gatgaaccca
    34921 ctcaaggcgt ggatgtaaat ggccaggtgg cgttatatga ccttattgac cagttgcgtc
    34981 gcgaactgga ttgtggcgtt ttaatggttt ctcacgatct gcatctggta atggcaaaaa
    35041 ccgatgaagt gctttgcctg aatcaccaca tttgttgttc cggcacaccg gaagttgttt
    35101 ccctgcatcc ggagtttatt tcaatgtttg gtcctcgtgg tgctgaacaa ctgggtatct
    35161 atcgccatca tcataatcat cgtcacgatt tacagggacg aattgttttg cgtcggggaa
    35221 atgatcgctc atgattgaat tattatttcc cggttggtta gccgggatca tgctcgcctg
    35281 tgccgcgggt ccgctgggtt cgtttgtagt ctggcgtcgt atgtcttatt tcggtgatac
    35341 gctggctcat gcctcattac ttggtgtcgc gtttggtttg ttgctggacg tgaatccatt
    35401 ctatgcggtg attgccgtta cgctgctgct ggcgggcggt ctggtatggc tggagaagcg
    35461 tccacagctg gcgatcgaca cgttattagg gattatggcg cacagtgccc tgtcgctggg
    35521 cctggtggtc gttagtctga tggctaacat tcgtgttgat ttgatgactt acctgttcgg
    35581 tgatttactg gcagtgacgc cagaagatct catctctatt gcaattggcg tggtcatcgt
    35641 ggtggctatt ttgttctggc aatggcgcaa tttgctgtcg atgacgatta gcccggatct
    35701 ggcgtttgtt gatggtgtga aattacagcg cgtgaaattg ttgttgatgc tggtgacggc
    35761 attgacgatt ggtgtagcga tgaaatttgt cggcgcgttg attattactt cgctgctgat
    35821 tattcctgca gctactgcac gtcgctttgc ccgtacgccg gaacagatgg ctggtgtcgc
    35881 tgttttggtg gggatggtgg cagtaactgg cggtttaacc ttttccgcgt tttacgatac
    35941 gccggcgggt ccgtcggtgg tgctatgtgc ggcactgtta tttattatca gtatgatgaa
    36001 aaagcaggcc agctaatcta tcgctgaaca catttgtcgg atgcggcgcg agcgccttat
    36061 ccgacctacg gttcgctatc tctggtatat gcctgataag acgcgaatag cgtcgcatca
    36121 ggcacactgc caatgtcgga tgcggtgcga gcgccttatc tgacctacgg ttcgctatct
    36181 ctcgtaggcc ggataagacg cgaatagcgt cgcatctggc acactgccaa tgtcggatgc
    36241 ggcgcgagcg ccttatccga cctacggttc gctatctctg gtaggcctga taagacgcga
    36301 atagcgtcgc atcaggcaca ctgccaatgt cggatgcggt gcgagcgacc aatccgactt
    36361 acggcatttc tggcggcgtt atgccaaagt gattccacgc ccgcgtcgtc gccatacgcc
    36421 cacgcggtgt acgctgcaaa aagccttgct gaatcaaata aggttccagc acatcctcaa
    36481 tggtttcacg ttcttcgcca atggctgccg caaggttatc cagacctacc ggcccaccaa
    36541 agaacttatc gattaccgcc agcaataact tgcggtccat ataatcgaaa ccttcagcat
    36601 ctacattcaa catatccaac gcctgagcag cgatatctgc cgagatggtg ccatcgtgct
    36661 tcacttcggc gaaatcacgc actcgacgca gcagacggtt ggcaatgcgc ggcgtaccgc
    36721 gagcgcgacg agcaacttcc agcgcgccgt catcactcat ctcaagcccc ataaagcgtg
    36781 cgctgcgact gacgatatat tgcagatccg gcacctgata aaactccaga cgttgcacaa
    36841 taccaaaacg gtcgcgcaac ggtgatgtca gcgaacctgc gcgcgtggtt gcaccaatca
    36901 gggtaaacgg cggcaaatca attttaatgg agcgtgccgc cggaccttca ccaatcatga
    36961 tatccagttg gtagtcttcc attgccgggt acagcacttc ttcaacaact ggcgataaac
    37021 ggtggatctc atcaataaac agcacgtcat gcggttcaag gttagtgagc atcgcagcca
    37081 aatcgcccgc cttttccagc accggaccag aagtcgtgcg taaattaacg cccatttcat
    37141 tggcgacaat gttggcaagc gtagttttac ccaaccccgg aggaccaaaa atcaacaaat
    37201 gatcgagggc atcgccgcgc agtttcgctg ctttgatgaa aatctccatc tgtgaacgaa
    37261 cctgcggctg accaacatac tcttccagta atttagggcg aatggcgcga tctgctacat
    37321 cttccggcaa agtggtaccg gcagaaatca gacggtctgc ttcaatcatc ctttacctca
    37381 taacgcggcg cgtagggctt cgcgaattaa tgtttcactg ctggcgtcag ggcgagcgat
    37441 tttgctcacc atgcggcttg cttcttgtgg tttatagccc agcgccacca gcgcggcaac
    37501 cgcttcttgt tcagcatcgt cggtcgccgg gctggcagga gacgtgagta ccaggtcggc
    37561 ggctggcgta aagagatcgc catgcaaacc tttaaatcgg tctttcattt ccacaatcaa
    37621 gcgttcggcg gtttttttgc caatacccgg cagtttcacc agtgccccca cttcttcacg
    37681 ctcaacggca ttaacgaact gctgcgctga cattccggag aggatcgcca gcgccaactt
    37741 cgggccgacg ccgttggttt tgatcaactc tttgaacaat gtgcgctctt gtttattgtt
    37801 aaaaccgtac agcagctgcg cgtcttcacg caccacaaag tgggtgaaaa cgatcgcttc
    37861 ctgtcccgct tcagggagtt cataaaaaca ggtcatcggc atatgcactt catagcctac
    37921 gccgcccact tcaattaaca ccagcggggg ttgtttttca atgatgatgc ctctgagtct
    37981 gcctatcaca tgacgctcct gcgtaatgaa tcaaagataa tgctgtatga taaaaaaatg
    38041 ctggatagat atccagcgaa ggatgaagaa aacttgcgag gtgtct
//
LOCUS       DAPGOF010000023        37899 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.023, whole genome shotgun sequence.
ACCESSION   DAPGOF010000023 DAPGOF010000000
VERSION     DAPGOF010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.023"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(222..776)
                     /locus_tag="RHV13_001051"
     CDS             complement(222..776)
                     /locus_tag="RHV13_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HDV1240606.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            1101..1307
                     /locus_tag="RHV13_001052"
     CDS             1101..1307
                     /locus_tag="RHV13_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HDV1240607.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(1369..2712)
                     /locus_tag="RHV13_001053"
     CDS             complement(1369..2712)
                     /locus_tag="RHV13_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDV1240608.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(3035..3673)
                     /gene="msrA"
                     /locus_tag="RHV13_001054"
     CDS             complement(3035..3673)
                     /gene="msrA"
                     /locus_tag="RHV13_001054"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDV1240609.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            3879..5612
                     /gene="tamA"
                     /locus_tag="RHV13_001055"
     CDS             3879..5612
                     /gene="tamA"
                     /locus_tag="RHV13_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HDV1240610.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            5609..9388
                     /gene="tamB"
                     /locus_tag="RHV13_001056"
     CDS             5609..9388
                     /gene="tamB"
                     /locus_tag="RHV13_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HDV1240611.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVNTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            9391..9732
                     /locus_tag="RHV13_001057"
     CDS             9391..9732
                     /locus_tag="RHV13_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HDV1240612.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            9944..10195
                     /gene="chpS"
                     /locus_tag="RHV13_001058"
     CDS             9944..10195
                     /gene="chpS"
                     /locus_tag="RHV13_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HDV1240613.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            10189..10539
                     /gene="chpB"
                     /locus_tag="RHV13_001059"
     CDS             10189..10539
                     /gene="chpB"
                     /locus_tag="RHV13_001059"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HDV1240614.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(10619..11149)
                     /gene="ppa"
                     /locus_tag="RHV13_001060"
     CDS             complement(10619..11149)
                     /gene="ppa"
                     /locus_tag="RHV13_001060"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HDV1240615.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            11459..12415
                     /gene="ytfQ"
                     /locus_tag="RHV13_001061"
     CDS             11459..12415
                     /gene="ytfQ"
                     /locus_tag="RHV13_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HDV1240616.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            12725..14227
                     /locus_tag="RHV13_001062"
     CDS             12725..14227
                     /locus_tag="RHV13_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDV1240617.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGFLRRKEMEKRATELMTSYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQNLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            14241..15263
                     /locus_tag="RHV13_001063"
     CDS             14241..15263
                     /locus_tag="RHV13_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV1240618.1"
                     /translation="MPQSLPDTTTPKRRFRWPTGMPQLVALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GVSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            15250..16245
                     /gene="yjfF"
                     /locus_tag="RHV13_001064"
     CDS             15250..16245
                     /gene="yjfF"
                     /locus_tag="RHV13_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HDV1240619.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(16278..17276)
                     /gene="fbp"
                     /locus_tag="RHV13_001065"
     CDS             complement(16278..17276)
                     /gene="fbp"
                     /locus_tag="RHV13_001065"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HDV1240620.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            17452..18825
                     /gene="mpl"
                     /locus_tag="RHV13_001066"
     CDS             17452..18825
                     /gene="mpl"
                     /locus_tag="RHV13_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HDV1240621.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(18976..19527)
                     /locus_tag="RHV13_001067"
     CDS             complement(18976..19527)
                     /locus_tag="RHV13_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HDV1240622.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            19621..20973
                     /gene="pmbA"
                     /locus_tag="RHV13_001068"
     CDS             19621..20973
                     /gene="pmbA"
                     /locus_tag="RHV13_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HDV1240623.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            21156..21542
                     /gene="cybC"
                     /locus_tag="RHV13_001069"
     CDS             21156..21542
                     /gene="cybC"
                     /locus_tag="RHV13_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HDV1240624.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEGKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(21587..22051)
                     /gene="nrdG"
                     /locus_tag="RHV13_001070"
     CDS             complement(21587..22051)
                     /gene="nrdG"
                     /locus_tag="RHV13_001070"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HDV1240625.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(22209..24347)
                     /gene="nrdD"
                     /locus_tag="RHV13_001071"
     CDS             complement(22209..24347)
                     /gene="nrdD"
                     /locus_tag="RHV13_001071"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDV1240626.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(24741..26396)
                     /gene="treC"
                     /locus_tag="RHV13_001072"
     CDS             complement(24741..26396)
                     /gene="treC"
                     /locus_tag="RHV13_001072"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDV1240627.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DESSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(26446..27867)
                     /gene="treB"
                     /locus_tag="RHV13_001073"
     CDS             complement(26446..27867)
                     /gene="treB"
                     /locus_tag="RHV13_001073"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HDV1240628.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(27986..28933)
                     /gene="treR"
                     /locus_tag="RHV13_001074"
     CDS             complement(27986..28933)
                     /gene="treR"
                     /locus_tag="RHV13_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HDV1240629.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            29118..29171
                     /gene="mgtL"
                     /locus_tag="RHV13_001075"
     CDS             29118..29171
                     /gene="mgtL"
                     /locus_tag="RHV13_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HDV1240630.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            29312..32008
                     /gene="mgtA"
                     /locus_tag="RHV13_001076"
     CDS             29312..32008
                     /gene="mgtA"
                     /locus_tag="RHV13_001076"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HDV1240631.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(32214..32600)
                     /gene="ridA"
                     /locus_tag="RHV13_001077"
     CDS             complement(32214..32600)
                     /gene="ridA"
                     /locus_tag="RHV13_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HDV1240632.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(32673..33134)
                     /gene="pyrI"
                     /locus_tag="RHV13_001078"
     CDS             complement(32673..33134)
                     /gene="pyrI"
                     /locus_tag="RHV13_001078"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HDV1240633.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(33147..34082)
                     /gene="pyrB"
                     /locus_tag="RHV13_001079"
     CDS             complement(33147..34082)
                     /gene="pyrB"
                     /locus_tag="RHV13_001079"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HDV1240634.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(34086..34220)
                     /gene="pyrL"
                     /locus_tag="RHV13_001080"
     CDS             complement(34086..34220)
                     /gene="pyrL"
                     /locus_tag="RHV13_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HDV1240635.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(34501..34896)
                     /locus_tag="RHV13_001081"
     CDS             complement(34501..34896)
                     /locus_tag="RHV13_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDV1240636.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            complement(35027..35740)
                     /gene="bdcA"
                     /locus_tag="RHV13_001082"
     CDS             complement(35027..35740)
                     /gene="bdcA"
                     /locus_tag="RHV13_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV1240637.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     KRLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGEALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSLMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            35811..36404
                     /locus_tag="RHV13_001083"
     CDS             35811..36404
                     /locus_tag="RHV13_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDV1240638.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAISAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPLARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            36549..37001
                     /gene="tabA"
                     /locus_tag="RHV13_001084"
     CDS             36549..37001
                     /gene="tabA"
                     /locus_tag="RHV13_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HDV1240639.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            37124..>37899
                     /locus_tag="RHV13_001085"
     CDS             37124..>37899
                     /locus_tag="RHV13_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDV1240640.1"
                     /translation="MSKISGWNFSQNITSADNCKQKNEDLDTWYVGMNDFARIAGGQN
                     SRSNILFPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIKSSSYIKNNDGSEIMQF
                     VMNNPEGERADLSKVEIEITLASAFNNGIREGHTVIIFTQPDGSTNRYEGKSFERKDE
                     SSLHLITNKILACYQREANKEIARLLNIPQELNNSQDLNNSQVSCKDSVDSTITDLLE
                     KPLNNALLAIRKEHLLLMPYVCNESISYLLGEKGILKEID"
BASE COUNT         9131 a         9447 c        10185 g         9136 t
ORIGIN      
        1 gccgaacaag tggatgcgat agtaagtgcg aatctggccg cagatagtga tattaccaaa
       61 gaaaagattt gcgagaaata taaatcgacc attgaggcgt ttaaaaaaga gcaactagcg
      121 ttaccagtat gagttcggcg taagaactca gagacttaat tcccctatcc catagataac
      181 gataggggaa aattattttt gcagaattta tgttattgcc attatttgag caacttttgc
      241 agcaggtcca tcacttgctg cacctcttcc tgtgtgagcg ccccgtcttt ggcccattgc
      301 acgcgaccgt ctttatccag caccactaca gcggaacttt cttcatccag ctgccaggca
      361 ccgcgcgcga cgccattgct atcgacaata aactgcgacc agggataaag ctttttatta
      421 ctctccagac tgctgcgcac aaacatgccg gaacccggaa ttgcgtcgtc ggtgttaaca
      481 atggtggtgg tctggtaacg atcgtgcggt aacttcgctg atttaatcgc ttcaatcagc
      541 gtcgcgtttt tctcttttgc agaggtgcga ccagcaatat gttgcagtac tcgcactttt
      601 cccactaact gcgcgctgtt ccaggttttg tagctaaact gatctttatc aagcaccaac
      661 tcgccccgat cggtaatgcc aatcggcggc actcgctgac cggtttcgaa ctgatgtgcg
      721 gaagccatca tcggcaacag caggcaggtg agtgccagaa tcttgcgtag ggtcatggtg
      781 tttccttctt atgatatgca ggtgatccga ccacttgggt ctgagtttta atcatatgtg
      841 ctatttatcg aatttcccgc aagtgtgatg ccagtttgcg gtcaagcgca caaatcatat
      901 gaaaaatgaa tgcttatact gaagaccgcg cttcggtaaa aagataattc tgaataattg
      961 taacctttag gtaaaaaaag ttatacgcgg tggaaacatt gcccggatag tctatagtca
     1021 ctaagcatta aaatttgcgc ctcataatag ttgggccgat tgtggcaccg cacaggcgta
     1081 atactcagca ggagataaca atgaaaattt tccaacggta caacccactt caggtggcga
     1141 agtacgtaaa gatcctgttc cgtggacggt tatacatcaa ggacgttggc gcttttgaat
     1201 ttgataaggg taagattctt atcccaaaag tgaaggataa gctgcatttg tcagtgatgt
     1261 ccgaagttaa ccgtcaggtt atgcgtctgc aaacagagat ggcttaacca aagtgctatg
     1321 cagtaataag acggctcctg attcaggagc cgttgatgtt tctgggggtt acgcgacgct
     1381 ttcttcttta tctttagcgt caggcagttt tggcgaaagg gcggtggcct tgctgtcgat
     1441 ccgggtcacc agcagctggt cgatgcggta gttatcgata tccaccactt caaatttgta
     1501 gccggcgaat ttcaccgaat cggtgcgttt cgggatctta cgcagcataa acatcataaa
     1561 gccgccgatg gtttcgtagt tgcccgactg cgggaattcg tcaatatcca gcacgcgcat
     1621 gacgtcgtca atcggagtgc cgccgtcaat cagccatgag ttctcatcac gggcgacaat
     1681 ctgctcttcc agcccctggc cgaccagatc gcccatcagc gtggtcatca cgtcgttgag
     1741 ggtgatgatc cccactacca gcgcgtactc gttcatgatc accgcgaagt cttcacctgc
     1801 ggttttaaaa ctttccaacg cctctgaaag ggttaacgta tccggcacaa tcagcgtgtt
     1861 gcgaatttgt acgccgctgt tcagtgccag gctttggtta gccagcacgc ggttcagcag
     1921 gtctttagaa tcgacatagc cgatgatgtg gtcaatatct tcattacaga cgaggaactt
     1981 agagtgcgga tgttccgcca ccttattctt cagactttgc tcatcttcgt ggagatcaaa
     2041 ccaaatcacg ttttcacgcg gtgtcattga cgacggaacg gtacgggatt ccagttcaaa
     2101 gacgttttca atcagctcgt gttcctgttt acgtaacacg cccgccagcg caccggcttc
     2161 cactaccgcg tagatgtcat cagaagtgat gtcatcttta cgtaccattg gcagtttgaa
     2221 aatacggaag attatgttcg ccaggccgtt gaagaaccac accagcgggg tgcaaacgta
     2281 caggcagaag cgcatcgggt tgatgatacg caaagccaca gcttctggcg caatcatacc
     2341 gatgcgtttc ggggttaaat ccgcaaacag gataaacatg ccagtcacta acgagaaaga
     2401 gagaataaag ctcagttgct cagagagctc tgccgacata tagcgggaga acaggctgtg
     2461 aaaagctgga gaaaatgccg catcaccgac gataccgccg agaatcgcca ctgcgttcag
     2521 accgatttgg accacggtaa agaacatgcc gggattttcc tgcatattca gaacgcgttg
     2581 ggcatttata ttgccttcat cagccagcag tttaagtttg attttgcgtg aggcggcaag
     2641 cgagatctcg gacatcgaga agaacgcact tacagcgatc aagcagagta tgactaaaat
     2701 actgtttaac atatcttatc cgacctttca ggccagatcc tcggaagggg aagttgatta
     2761 atttgtgtga aatacacatt gaaagccgat tcgaagaaag tgaaccggca ttttcagtgg
     2821 ctagtatagc gtaaggtact gtaaagccgc cagagggtta aaattcagac agctgaaaaa
     2881 tgcaaaactg cctgatacgc tatgccgatc aggcctacgc agtccttgca atatattgaa
     2941 tttggacgat tttgtaggcc tggtaagtcg tttacgccgc atcaggcata gacaaagagc
     3001 actttatcaa caatctacat ttgtacccgt aacgctatgc ttccggtggc agacagacgc
     3061 caattccgcc aattccacag taaccatacg ggtttttatg cagatattgc tggtggtcat
     3121 cttcggcata ataaaacggt gtggcgttag cgatttccgt ggtgatgtga cgatcgtcat
     3181 cggcggcaag catcgccgcc tgaaaacgtt ccagactggc gcgagctgcg gcatcctgtt
     3241 ctggggtcag cggataaatc gctgaacgat actgcgtgcc gtggtcattg ccctgacgca
     3301 ttccctgggc gggatcgtga ttctcccaaa atacctgtag caactgctca tagctgatga
     3361 cggaaggatc gtaaacaatg cgtaccgctt cggcatgacc cgtatcaccg gagcacactt
     3421 cccgataagt cggatttggc gtatagccgc cggtatagcc tgcggcggtg ctgtaaacgc
     3481 cgggtaactg ccagaacaga cgctccacac cccagaaaca acccatcgca aaaatggcaa
     3541 tctccattcc gtcaggtaca ttggtcattg agtgaccgtt gaccgcatgc agcgtggcta
     3601 cgggcatcgg ggtgttacgt ccaggcaggg catcggcggg ggaaaccaga tgctttttat
     3661 caaataaact catggtgtcg ctctcccgaa aatcggtcat tggggttaag gttgtaacaa
     3721 gaggcgtatt tgcacacaat aaccgccgtg aataggtcta aagtaaaaca taagaaatat
     3781 ttggggttta gtctgctttt taatccatat tactggattt ttgttaagcc gtttaacggc
     3841 gttccagggg caggaaaaaa ggatattcag gagaaaatgt gcgctatatc cgacagttat
     3901 gctgtgtaag cttactctgc ttaagcggat ctgccgtcgc cgcgaacgtc cgtctacagg
     3961 tcgaggggtt atcgggacag ctggaaaaga acgttcgtgc gcagctttct acgattgaaa
     4021 gtgatgaagt gacgccagac cgtcgctttc gcgcacgcgt cgatgatgcc atccgcgaag
     4081 gtctgaaagc gctgggttat taccagccga ccattgaatt tgatctccgt ccaccgccaa
     4141 agaaagggcg gcaggtattg atcgccaaag tcacgccagg cgtgccggtg ttaattggcg
     4201 gcaccgatgt ggtattgcgc ggcggcgcgc ggaccgataa agactatttg aaattgctcg
     4261 atactcgccc ggctattggc acggtactga accagggcga ttatgaaaat ttcaaaaagt
     4321 ccttaaccag cattgcgttg cgtaaaggtt atttcgatag cgaatttacc aaagcgcagc
     4381 tgggcattgc gctcggcctg cataaagcct tctgggatat tgattataac agtggcgaac
     4441 gttaccgctt tgggcatgtg acctttgaag gatcacaaat ccgcgatgaa tacctgcaaa
     4501 atctggtgcc gtttaaagag ggcgatgagt acgaatcgaa agatctggcg gaacttaacc
     4561 gtcgactttc tgctaccggc tggtttaact cggtggtggt ggctccacaa tttgataaag
     4621 cgcgcgaaac gaaagtatta cccttgacgg gcgtggtttc gccgcgaact gaaaacacca
     4681 tcgaaaccgg ggtcggttac tctacggacg tgggaccgcg cgtgaaagcg acgtggaaaa
     4741 agccgtggat gaactcttat ggtcacagtc tgaccaccag taccagtatt tccgcgccgg
     4801 aacagaccct cgacttcagc tataaaatgc cgctgctgaa gaatccactg gaacaatatt
     4861 atttggtgca gggcggtttt aagcgcactg acctgaacga taccgaatct gactccacta
     4921 cgctggtggc ttctcgctac tgggatctct ccagcggctg gcagcgtgcc attaacctgc
     4981 gctggagcct cgaccacttt acccagggtg aaattaccaa caccacgatg ctgttttatc
     5041 ctggggtgat gattagccgc acgcgttctc gtggtggcct gatgccaacc tggggcgact
     5101 cgcaacgcta ctctatcgac tactccaaca cggcctgggg ctcagatgtc gatttctccg
     5161 ttttccaggc gcagaacgtc tggatccgca cactgtacga tcgccatcgt tttgtgacac
     5221 gcggcacgct gggctggatt gaaacgggtg atttcgacaa agtaccgccg gatctgcgtt
     5281 tcttcgccgg gggcgatcgc agtattcgcg gctacaaata caaatctatc gccccgaaat
     5341 acgccaacgg tgacctgaaa ggggcctcga agctgataac cggatcgctg gaataccagt
     5401 acaacgtgac cggaaaatgg tggggcgcgg tgtttgtcga tagtggcgaa gcggtaagcg
     5461 atattcgccg cagcgacttt aaaaccggta ccggggtcgg cgtacgctgg gaatcgccgg
     5521 tcgggccaat taaactcgat tttgccgtac cggtcgcgga taaagacgag cacgggttac
     5581 agttttacat cggtctgggg ccagaattat gagtttatgg aaaaaaatca gcctcggcgt
     5641 ggttatcgtt atcttactgt tgctgggatc ggtggcgttt ctggtgggca ccaccagcgg
     5701 cctgcatctt gtatttaaag cggcggatcg ctgggtgcca ggactggata ttggcaaggt
     5761 caccggcggc tggcgcgatc tcaccttgtc tgacgttcgt tatgagcagc caggcgtggc
     5821 ggtaaaagcg ggtaatctgc atctggcggt cggtcttgag tgcctgtgga acagtagcgt
     5881 ttgtattaat gacctggcgc tgaaagacat tcaggtcaac atcgacagta aaaaaatgcc
     5941 cccttctgaa caggttgaag aagaggaaga tagcggtccg ctggatctct ccacgccgta
     6001 tcccatcacc ctgacacggg tggcgctgga taacgtcaac atcaagattg atgacaccac
     6061 ggtatcggtg atggacttca cctccggcct gaactggcag gagaaaaccc tgaccctgaa
     6121 accgacgtcg ctgaaaggcc tgttgattgc cctgccgaaa gtggcggaag tggcgcagga
     6181 agaagtggtc gaaccgaaaa ttgaaaatcc gcagccggat gaaaagccgc tcggcgaaac
     6241 gttgaaagat ctcttttctc gtccggtatt gccggaaatg accgacgtgc atttgccgct
     6301 taacctgaac attgaagagt ttaagggcga gcagctgcgc gtgacgggcg acacggacat
     6361 caccgtgaac accatgctgc tgaaagtaag cagcatcgac ggtaatacta aactggacgc
     6421 cctggatatc gattccaatc aagggatcgt caacgccagc ggcacggcac agctgtcaga
     6481 caactggccg gtggatatca ccctcaacag cacactaaac gtggagccgt tgaaaggtga
     6541 aaaagtgaag ctgaaagtgg gcggcgcgct gcgcgaacag ctggagattg gcgttaacct
     6601 ttccggtccg gtggatatgg atttacgcgc ccagacgcga ctggcggaag ctggattgcc
     6661 gctcaacgtg gaagtgaaca gcaaacagct ttactggccg ttcactggtg agaagcagta
     6721 tcaggcggat gatctgaaac tgaaacttac cggcaaaatg accgattaca cgctctctat
     6781 gcgtacggca gtgaagggac aggagatccc gcccgcgact attaccctcg acgcgaaagg
     6841 taatgaacag caggtcaatc tcgacaaact caccgttgcg gcgctggaag gcaaaactga
     6901 actcaaggcg ttgctcgact ggcaacaggc cattagttgg cgcggtgagc taacgcttaa
     6961 tggcattaac accgccaaag agttcccgga gtggccgtcg aaactcaatg gcttgattaa
     7021 aacccgcggt agcctgtacg gcggcacctg gcagatggag gtgccagaac tgaagctgac
     7081 cggtaacgtc aaacagaaca aagtgaacgt tgacggcacg ctgaaaggca acagttatat
     7141 gcagtggatg atcccagggc ttcatctgga actggggcca aacagtgccg aagtgaaagg
     7201 cgagctgggg gtaaaagatc tcaatcttga tgccaccatc aacgcgccgg ggctggataa
     7261 cgcgctgccg gggcttggcg gtacagcgaa agggctggtg aaagtacgcg gcacggtgga
     7321 agcgccacaa ctactggcag atatcaccgc gcgcggcctg cgctggcagg aactttccgt
     7381 ggcgcaggtt cgcgtggaag gcgacattaa atccaccgat cagatcgccg ggaaactcga
     7441 cgtacgcgtt gagcaaattt cgcagccgga tgtaaatatc aacctcgtca ccctgaatgc
     7501 caaaggcagc gaaaagcagc acgagctaca gttgcggatt cagggcgagc ctgtctccgg
     7561 gcagcttaat ctggcaggaa gttttgatcg caaagaagaa cgctggaagg gaactcttag
     7621 caatacccgc ttccagacgc cggtcggccc gtggtcgctg acccgcgata ttgcgctgga
     7681 ttaccgcaat aaggagcaaa aaatcagcat cgggccacac tgttggctta acccgaatgc
     7741 ggagctgtgc gtgccgcaaa ctatcgatgc gggggccgaa gggcgtgcgg tggtgaatct
     7801 caaccgcttc gacctcgcca tgctgaaacc gtttatgcca gaaaccactc aggccagcgg
     7861 tatcttcacg ggtaaagcgg atgttgcctg ggacaccacg aaagaggggc tgccgcaggg
     7921 cagtatcacc ctttcggggc gtaacgtgca ggtaacgcaa accgtcaacg atgcggcgct
     7981 gcccgtggcg tttcagacgc tgaatctgac ggcggaattg cgtaacaacc gtgccgaatt
     8041 gggctggacc atccgcctga ccaataacgg ccagtttgat ggacaggtgc aggtgaccga
     8101 tccgcaaggc cgccgtaatc ttggtggcaa cgtcaatatc cgtaacttca accttgcgat
     8161 gataaacccc atctttaccc gtggggaaaa agcagcgggg atggtgagtg ccaacttgcg
     8221 tctgggtggt gatgtgcaaa gcccgcagtt gtttggtcag cttcaggtta cgggtgtgga
     8281 tatcgacggc aactttatgc cgtttgatat gcaaccgagc cagcttgcgg tcaactttaa
     8341 cggtatgcgc tcgacgcttg ccggtacagt acggacccag cagggtgaaa tctacctgaa
     8401 cggtgatgcc gactggagcc aaattgaaaa ctggcgggcg cgggtaacgg cgaaaggcag
     8461 taaagtgcgg atcaccgtgc cgccgatggt acgaatggat gtatcgccag atgttgtatt
     8521 cgaggctaca ccaaacctgt ttaccctcga tggtcgcgtg gatgtccctt gggcgcgcat
     8581 cgtggtgcac gatctgccgg aaagcgcagt aggcgtctcc agcgatgtgg tgatgcttaa
     8641 cgataacctg caaccggaag agccgaaaac ggcgtcgatt ccgattaaca gtaacctgat
     8701 tgtccacgtt ggcaataatg tgcgcattga cgcctttggc ctgaaagcgc ggctgacggg
     8761 cgatcttaac gtcgttcagg acaaacaagg gctgggcctg aacgggcaga tcaacatccc
     8821 tgaagggcgc ttccatgcct atggtcagga tctgattgtg cgtaagggtg agttactgtt
     8881 ctctggtccg ccggatcaac cgtatcttaa tattgaagct attcgtaacc cggatgctac
     8941 agaagacgac gtaatcgccg gagttcgcgt cactggtctg gcggacgaac cgaaagcgga
     9001 gatcttctct gacccggcga tgtcgcaaca agctgcattg tcttatttgc tacgtggaca
     9061 agggctggag agcgatcaga gcgacagtgc ggcaatgact tcgatgctga ttggtctggg
     9121 ggttgcgcaa agtggccaga ttgtgggtaa aatcggcgag acgtttggcg taagcaattt
     9181 agcgctcgac acccagggag taggcgactc ctcccaggta gtggtcagcg gctatgtatt
     9241 gccaggtctg caagtgaaat atggcgtggg tatatttgac tctatagcaa cactcacgtt
     9301 acgttatcgc ctgatgccta agctatatct ggaagccgtg tctggtgtag accaggcact
     9361 ggatttgctc tatcagttcg agttttagca atgcgaatat ttgtctacgg cagtttacgc
     9421 cacaaacaag gcaacagtca ctggatgacc aatgcccagt tactgggcga tttcagtatc
     9481 gataactacc agttgtatag cctgggccac tatccaggcg cagttccggg gaacggaaca
     9541 gtacacggtg aagtttatcg tattgacaac gccacgctgg ccgaacttga tgccttgcgc
     9601 accaggggcg gtgaatacgc gcgccagttg attcagacgc cgtacgggag tgcatggatg
     9661 tacgtttatc aacgacccgt cgatggatta aagctaattg aaagcggcga ctggttagac
     9721 agggataagt aaccatatgc atacgccacc ttcgggtggc gttgtttttt gcgagacgac
     9781 tcgcattctg ttttgtaatt ccctcaccct ttgcttttct ctccgagccg ctttccatat
     9841 ctgttaacgc ataaaaaact ctgctggcgt tcacaaatgc gcaggggtaa aacgtttcct
     9901 gtagcaccgt gagttatact ttgtataact taaggaggtg cagatgcgta ttaccataaa
     9961 aagatggggg aacagtgcag gtatggtcat tcccaatatc gtaatgaaag aacttaactt
    10021 acagccgggg cagagcgtgg aagcgcaggt gagcaacaac caactgattc tgacacccat
    10081 ctccaggcgc tactcgcttg atgaactgct ggcacagtgt gacatgaacg ccgcggaact
    10141 tagcgagcag gatgtctggg gtaaatccac ccctgcgggt gacgaaatat ggtaaagaaa
    10201 agtgaatttg aacggggaga cattgtgctg gttggctttg atccagcaag cggccatgaa
    10261 cagcaaggtg ctggtcgacc tgcgcttgtg ctctccgttc aagcctttaa tcaactggga
    10321 atgacgctgg tggcccccat tacgcagggc ggaaattttg cccgttatgc cggatttagc
    10381 gttcctttac attgcgaaga aggcgatgtg cacggcgtgg tgctggtgaa tcaggtgcgg
    10441 atgatggatc tacgcgcccg gctggcaaag cgtattggtc tggctgcgga tgaggtggtg
    10501 gaagaggcgt tattacgctt gcaggcggtg gtggaataag gtgtgtttat ttatcgctgg
    10561 cataaaaaaa cccttactaa ccgaagctcg gcgttcaggg ttattgcgct agaagaactt
    10621 atttattctt tgcgcgctcg aaggaagcaa cgatttcagc tttagcggct tctgcgtttt
    10681 cccaaccttc aactttcacc cacttgcctt tttcgaggtc tttgtagtgc tcgaagaagt
    10741 gagcgatctg cgctttcagc agctctggca gatcgttaac gtctttaatg tgatcgtatt
    10801 ctttgctcag cttgctgtgc ggaaccgcaa ccagtttcgc atcttcaccg gcttcgtcgg
    10861 tcattttcag aacgccaacc ggacggcaac ggatcacaga acccggctgc agcgggtacg
    10921 gagtcgggac cagtacgtca accgggtcac cgtccagaga cagggtgtgg ttgatgtaac
    10981 cgtagttgca cggatagaac atcgcggtgg acatgaagcg gtcaacgaac agtgcgccgc
    11041 tctctttgtc gatttcgtat ttgatcggat ctgcgttagc cgggatctca ataacaacgt
    11101 agatgtcttc cggcagatct ttacccgcag ggacgttgag taagctcatg tctgtttcct
    11161 ttaaaaatat gtggcaaaca agtgccgagt attatagcca actcgcgccg aatgtcttcg
    11221 cttgttttcg tcttcatttt cctttttcgc cagttttaag acggtataca gagcaggaaa
    11281 atccataacc gtagccgcat ttttcatagt gagatgaaag cgattacaaa cttgtgatta
    11341 acgttttatt tacttttatg aagtgtgatg taacgcaatc cgttacataa cgaattgtct
    11401 atagtttttc cgcgaacatc ttttcaccaa taataattac cccgacgagg acaaccctat
    11461 gtggaaacgc ttacttgtag tctctgcagt ctcggcagcc atgtcgtcta tggcgttggc
    11521 cgctccatta accgtaggat tttcgcaggt cggatcggaa tccggctggc gcgctgcaga
    11581 aaccaatgtg gcgaaaagtg aggccgaaaa acgcggaatt acgctgaaaa ttgccgatgg
    11641 tcagcaaaag caggaaaacc agattaaagc ggtacgttcc ttcgtcgcgc aaggggtgga
    11701 tgcgatcttt atcgctccgg tggtcgcgac aggttgggaa ccggtattaa aagaggcgaa
    11761 agatgccgaa atcccggtct tcttgctcga ccgttccatc gatgtgaaag acaaatctct
    11821 ctatatgacc accgttactg ccgacaacat cctcgaaggt aagttgatcg gtgactggct
    11881 ggtaaaagaa gtgaatggca aaccatgcaa cgtggtggag ctgcagggca ccgttggggc
    11941 cagcgtcgcc attgaccgca agaaaggctt tgccgaagcc attaagaatg cgccaaatat
    12001 caaaattatc cgctcgcagt caggtgactt tacccgcagt aaaggcaaag aagtcatgga
    12061 aagctttatc aaagcggaaa acaacggcaa aaacatctgc atggtttacg cccataacga
    12121 tgacatggtg attggcgcaa ttcaggcaat taaagaagcg ggcctgaaac cgggcaaaga
    12181 tatcctcacg ggttccattg acggtgtacc ggacatctac aaagcgatga ttgatggcga
    12241 agcgaacgcc agtgttgaac tgacgccgaa tatggcggga cccgcttttg acgcgctgga
    12301 gaaatacaaa aaagacggca ccatgcctga aaagctgacg ttaaccaaat ccacccttta
    12361 cctgcctgat accgcaaaag aagagttaga gaagaagaaa aatatggggt attgagggtt
    12421 gctatgcctg atgccgattc gtaggccgga taaggcgctc gcgccgcatc cggcggtggt
    12481 gcgctgaagc ctgatgcgac gcttaccgcg tcttatcatg cctacgagtt cggtgctctg
    12541 tgtaggccgg ataaggcgtt cacgccgcat ccggcagtgg tgcaacaccg gacttaacac
    12601 cgtgcacaat ctgtcccctc gccccttcgg ggagagggtt agggtgaggg ggacaaacgg
    12661 cgcagctctt aacatcaggc acgatctatt cccagacttt tagagcgtgg ctaaggggga
    12721 aaccatgacg accgaccaac accaggaaat cctccgcacc gaaggattaa gtaaattttt
    12781 ccccggcgtc aaagcgttag acaacgttga tttcagcctg cgccgtggcg aaatcatggc
    12841 gctgctcggt gaaaacgggg cgggaaaatc gacgctaatc aaagcattaa ccggtgtata
    12901 ccacgccgat cgcggcacca tctggctgga aggccaggct atctcaccga aaaataccgc
    12961 ccatgcacaa caactcggca tcggaaccgt ttatcaggaa gtcaacctgc tacccaatat
    13021 gtcggtcgct gataatctat tcataggtcg cgaacccaaa cgtttcggct ttctacgccg
    13081 caaagagatg gaaaagcgtg ccaccgaact gatgacatct tacggtttct ccctcgacgt
    13141 gcgtgaaccg cttaaccgct tttcagtcgc gatgcagcaa atcgtcgcca tttgccgggc
    13201 tatcgatctc tctgccaaag tgctgatcct cgatgaaccc accgccagtc tcgacactca
    13261 ggaagtagaa ttactgtttg gcctgatgcg tcagttgcgc gatcgcggcg tcagcctgat
    13321 ctttgtcact cactttctcg atcaggtcta tcaggtcagc gatcggatca ccgttttacg
    13381 caacggcagt ttcgtaggct gtcgggaaac gcgcgagcta ccgcagatcg aactggtaaa
    13441 aatgatgctg gggcgcgagc tggataccca cgcgctacag cgtgccgggc gaacattgtt
    13501 gagcgacaaa cccgttgccg cgttcaaaaa ttacggcaaa aaaggaacga tagcaccgtt
    13561 tgatctcgaa gtgcgccccg gcgagatcgt cggcctggca ggcttgctgg gatccggacg
    13621 taccgaaacc gccgaagtga tcttcggtat caaacctgct gacagcggca cggcgttgat
    13681 caaaggcaaa ccgcaaaacc tgcgatcgcc acatcaggct tcggtactgg gcatcggctt
    13741 ctgcccggaa gacaggaaaa ccgatggcat catcgctgcc gcctcggtgc gggaaaatat
    13801 catcctcgct ctccaggccc agcgcggctg gctacgaccc atttcccgca aagagcagca
    13861 agagattgcc gaacgcttta tccgccagct tggcattcgc acaccttcaa ctgaacaacc
    13921 gattgaattt ctctccggcg gcaatcagca aaaagtgttg ctttcacgtt ggctactgac
    13981 ccgaccgcaa tttctgatcc tcgatgagcc aacccgtggc attgatgttg gtgcccacgc
    14041 cgagatcatc cgcctgattg aaacgctatg cgccgatggt ctggcgctgc tggtgatctc
    14101 ctccgaactg gaagagctgg tgggctatgc cgaccgggtg atcatcatgc gcgatcgcaa
    14161 acaggtggcg gagatcccgc tggcagagct ttccgttccg gcgatcatga acgccattgc
    14221 ggcgtaagga gaacagtgtg atgcctcaat ctcttccgga caccactacg ccaaaaaggc
    14281 gctttcgctg gccgacgggg atgccgcagc tggtagcact attgctggtg ctgctggtcg
    14341 acagcctggt agccccgcat ttctggcagg tagtgcttca ggatgggcgt ttgttcggta
    14401 gccccataga cattcttaac cgtgcagccc ccgttgcgtt actggcgatt ggtatgacgc
    14461 tggtgatcgc caccggtggg attgatctct ccgtaggggc ggtgatggct atcgccggag
    14521 ccacaacggc tgcgatgacg gtcgcgggag tcagcctgcc gattgttttg ttaagcgccc
    14581 tgggcaccgg catcctggcg ggattgtgga acggcatact ggtagcgatc ctcaaaattc
    14641 agccatttgt cgccacccta atcctgatgg tcgccgggcg cggcgtggcg caactgatca
    14701 cctccggcca gatcgtcacg tttaactcgc cggatctctc atggtttggc agcggatcgc
    14761 tgttgttcct gccaacgccg gtcattatcg cggtgctgac gcttctcctg ttctggctgt
    14821 tgacccgcaa aacggcgctg gggatgttta tcgaagccgt tggtatcaac attcgggcgg
    14881 caaaaaatgc cggggtaaac acgcgaatca tcgtcatgct cacctacgtg ttgagcgggc
    14941 tatgtgcggc gattgcgggc attatcgtgg cggcggatat tcgcggtgcc gatgccaaca
    15001 acgccggatt atggctggag ctggacgcca ttctcgcggt ggtgattggc ggcggatcgc
    15061 tgatgggcgg gcgctttaac ctgctgctct cagtcgtggg ggcgctgatt attcagggga
    15121 tgaacaccgg aattttactt tctggctttc cgccggagat gaaccaggtc gtaaaagcgg
    15181 tggtggtgct ttgtgtgctg attgttcagt cgcaacgctt tatcagtctg attaaaggag
    15241 tacgtagcca tgataaaacg taacttgccg ctgatgatca ccatcggcgt ctttgtgctg
    15301 ggctatctct actgcctgac ccagtttccc ggttttgctt ccacaagagt gatctgcaat
    15361 atcctgaccg ataacgcctt tcttgggata atcgccgttg gcatgacctt tgtgatcctc
    15421 tctggcggta tcgatctctc cgtcggttcg gtgatcgcct ttactggcgt gtttctggca
    15481 aaagtgattg gcgatttcgg cctctcgccg ctgctggcgt ttccgctagt gctggtgatg
    15541 ggctgtgcct ttggcgcatt tatggggctt ttgatcgacg ccctgaagat cccggcattt
    15601 atcattaccc tggcaggaat gttctttttg cgcggcgtca gctatctcgt ttcagaagag
    15661 tcgatcccga taaaccatcc catttatgac acgctctcaa gccttgcgtg gaaaatccct
    15721 ggcggcggtc gcttaagtgc gatgggactg ctgatgctgg cggtggtggt tatcggcata
    15781 ttcctcgcgc atcgtacccg ttttggtaat caggtatacg ccattggcgg caacgcaacg
    15841 tcggcaaacc tgatggggat ttctacccgc agcaccacta ttcgcattta tatgctctcc
    15901 accggactgg caacgctggc ggggattgtc ttctcgattt atacccaggc cggatatgcg
    15961 ctggcgggcg taggtgtgga actggatgct atcgcctcag tggtaattgg cggtacgctt
    16021 ttgagcggtg gcgttggaac ggtattaggg acgctttttg gcgtggcgat tcaggggcta
    16081 attcagactt atatcaactt tgacggtacg ctgagttcct ggtggacgaa aatcgccatc
    16141 ggcattttgc tgttcatttt tattgcctta cagcgtggat taacggtgct gtgggagaat
    16201 cgtcagagtt cgccagtgac aagagtcaat attgcgcagc gataaaaacg cctctccgtg
    16261 tggagaggcg caggagatta cgcgtccggg aattcacgga taaagcgttc gacatcttca
    16321 accatatggt cgttgccgac aaagaacgaa cggcgctggt gcagagtttc cgggatgata
    16381 tccagaatac gctctttgcc atcgctcgct ttaccgcccg cttgttccgc caggaatgcc
    16441 atcgggttgc actcatacag caaacgcagt ttgccgtccg ggtggctggc ggtgcttggg
    16501 tagagataaa taccgccttt cagcaggtta cggtggaaat ccgcgaccag tgaaccgata
    16561 taacgtgagg tatacgggcg gttggtggat ttatcttctt cctggcagaa tttaatgtac
    16621 ttcttcaccc cgttcggaaa cttaatgtag tttccttcgt tgatggagta ggttttgcct
    16681 ttctccggga agcgcatccg ttcctggcac aggcagaaaa cgcccagcga aggatcgtaa
    16741 gtaaaggcgt gaacaccgca tccggtggtg taaaccagca tggtagagga gccgtatacc
    16801 acgtaacctg ccgcaacctg tttgttacca ggctggagga aatcttcttc cgttaccggc
    16861 gtgccaacag gcgtaacgcg gcggtagatg gagaaaatgg taccgacaga gacgttaaca
    16921 tcgatgttgg acgagccatc cagggggtcc atcagcacca cgtattttgc gtgttcacag
    16981 ccttcaaaga cgacaatctc atcttcttct tcagaggcaa tgcccgcaac gatatcgcgt
    17041 gctttcagtg cggctttcag tttttcatta gcgaacaagt cgagtttctg ctgaacctcg
    17101 ccctgcacgt tctcagcacc gctggcaccc aggatatcaa ccagtcctgc tttgttgata
    17161 tcgcgatgga taatcttggc gcccagtttt attgccgaca gcaaagcagt gagctcaccg
    17221 gtagcatgag aaaactcgtg ctgcttttcg acaataaatt cacctaacgt tttcataaaa
    17281 ctttccctgc aatgtttatg gagtaaagcg accgcaacaa tcttaacaaa taatctcaat
    17341 gttgcgctca ggtgaatcgc gccagcaaat tgcggattat cctgaaatgc gtttctcact
    17401 tgcccgacat atgcgtaaaa tgagcggcag attaaaaaag gatagtgacg tatgcgcatt
    17461 catattttag gaatttgtgg cacatttatg ggcggtctgg cgatgctggc gcgccagtta
    17521 ggccatgaag taacgggttc ggacgccaat gtgtatccgc cgatgagcac cttacttgag
    17581 aagcaaggca ttgagctgat tcagggttac gatgccagcc agctcgatcc gcagccggat
    17641 ctggtgatta ttggcaacgc catgacccgt ggaaatccgt gtgtggaagc ggtactggaa
    17701 aaaaacatcc cttatatgtc aggtccacag tggctgcacg attttgtgct gcgcgaccgc
    17761 tgggtgctgg ccgttgccgg tacacacggc aaaactacca ccgcgggaat ggcgacctgg
    17821 attctggaac agtgcggtta caaaccggga tttgtgatcg gcggtgtgcc ggggaacttt
    17881 gaggtttcgg cgcgtctggg cgaaagcgac ttctttgtta tcgaagcgga tgagtatgac
    17941 tgcgccttct tcgacaaacg ctctaaattt gtccattact gcccgcgtac gctgatcctc
    18001 aacaaccttg agttcgatca cgccgatatc tttgacgacc tgaaagcgat ccagaaacag
    18061 ttccaccatc tggtgcgtat cgttccgggg cagggccgta ttatctggcc ggaaaatgac
    18121 atcaacctga aacagaccat ggcgatgggc tgctggagcg agcaggagct ggtgggtgag
    18181 cagggtcact ggcaggcgaa aaagctgacc actgacgctt ctgaatggga agtcttgctg
    18241 gatggcgaaa aagtgggcga agtgaaatgg tcgctggtgg gcgaacataa tatgcacaat
    18301 ggcctgatgg cgattgcggc ggctcgccat gttggtgtag cgcctgcaga tgccgctaac
    18361 gcgctgggtt cgtttattaa cgctcgtcgc cgtctggagt tgcgtggtga agcgaatggc
    18421 gtgacggtat atgacgattt tgcccatcac ccgacggcaa ttctggcaac gcttgcggcg
    18481 ctgcgtggca aagttggtgg tacggcgcgc attattgctg tgctggagcc gcgctcgaat
    18541 accatgaaaa tggggatctg caaagacgat ctggcacctt cattaggtcg tgccgatgaa
    18601 gtcttcctgc tgcaaccggc gcatattccg tggcaggtgg cagaagtggc agaagcctgc
    18661 gttcagcctg cacactggag tggcgatgtg gatacgctgg cagatatggt ggtgaaaacc
    18721 gctcagcctg gcgaccatat tctggtcatg agcaacggcg gttttggtgg gatccatcag
    18781 aaactgctgg atggtctggc gaagaaggcg gaagctgcgc agtaattcgg cctcagcctg
    18841 agatagcatt gccggataag gcgtttacgc cgcatccggc atttgagcat agtgcctgat
    18901 gcgacgcttg atgcgtctta tccggcctac aggtgcaccg catccggcaa accatactca
    18961 catcaacaac gaaaattacc cttcattctc cgccagttcg cgcagatact ggaaaatctg
    19021 gcgtgcggat ttcggcggct tattcccttc tttctctttc ttcgcgttac ggatcagagt
    19081 acgcagctgc tgacgatcgg catcgggcca cagattcaat acttcagcga tggcgtcatc
    19141 accttgatcg atcagacgat cgcgcaggtt ttccagttta tggaacagca ccacctgctg
    19201 gttgtgacgg tttttcagct tatccagcgc ctgacgaata ggctctacgt cgcgctggcg
    19261 cagcatctta ccgatgagct gcaactggcg gcggcgacct tccatcttaa tacgctgggc
    19321 gagctcaata gccgcgcgca gatccgcatc taacgggatc ttatccagcg cgtttttccc
    19381 cagatcaaca atttccgcgc caaggcgttt tagctcctcg gcatcacgtt taatttcact
    19441 tttactgacc cagataattt catcgtcttc gtcttcgatg tcatcaccgg gaacgtcgtc
    19501 gagccagtct tcgggctgct tagtcatctc aggctcctta aaaaaagagg ctaatgttac
    19561 cagttaagat gcgcactgaa aaacggttct ctgttagact tcagagaaac tctctacatt
    19621 atggcacttg caatgaaagt aatctctcaa gttgaagcgc agcgcaagat tctggaagaa
    19681 gcagtttcca ctgcgctgga gttggcctca ggcaaatcgg acggtgcgga agttgccgtc
    19741 agcaagacca ccggcattag cgtaagcacg cgttatggtg aagtggagaa tgttgaattc
    19801 aatagcgatg gcgcgctggg gatcactgtt tatcaccaga accgcaaagg tagcgcatca
    19861 tccaccgatt taagcccgca ggccattgcc cgcaccgtac aggcggcgct ggatattgcc
    19921 cgttatacct cgccagatcc ctgtgccggc gtggcagaca aagagctgct ggcctttgac
    19981 gcaccagatc tcgacttgtt ccaccctgcg gaagtttccc cggatgaagc cattgaactg
    20041 gcggcccgcg cagaacaggc ggcattgcag gcggacaaac gcattaccaa taccgaaggc
    20101 ggcagtttta acagccacta cggtgtcaaa gtttttggca acagccacgg catgttgcag
    20161 ggttactgct ctacgcgtca ttcactctcc agctgtgtaa ttgccgaaga aaatggcgat
    20221 atggagcgtg attacgccta caccattggc cgcgcgatga gcgatctgca aacgccagag
    20281 tgggtcgggg ccgactgtgc tcgccgtact ttatcgcgcc tgtcaccgcg taaactctcc
    20341 accatgaaag cgccagtcat ttttgccaat gaagtggcaa ccgggctttt tggccatctg
    20401 gtgggggcga tagcgggtgg ctcggtttac cgtaaatcta cctttctgct ggattcgctg
    20461 ggtaaacaaa ttctgccgga ctggctgacc attgaagagc atccgcatct gctgaaaggg
    20521 ctggcgtcga cgccattcga cagcgaaggt gtgcgcaccg agcgtcgcga tattattaaa
    20581 gatggcatcc tgactcagtg gctgctgacc agctactcgg cgcggaaact ggggctgaaa
    20641 agcaccggac atgcgggcgg tattcacaac tggcggattg ccggacaagg gctaagcttc
    20701 gaacaaatgc tcaaagagat gggcaccggg ctggtggtga cggagctgat gggccagggc
    20761 gtgagtgcca ttactggtga ttattcccgt ggtgctgcgg gcttctgggt agagaatggc
    20821 gaaattcagt atccggttag tgaaatcacc atcgcaggta atttaaaaga tatgtggcgc
    20881 aatattgtca ccgtcggtaa cgatattgaa acacgcagta atatacagtg tggttctgtg
    20941 ctgttgccgg agatgaaaat cgccgggcag taaaaaactg gcgcgacctg ccgcgctata
    21001 aactaaaaaa ttacacaaaa tcattcgcac tgcatcgaga cggaaagtga atgagggccg
    21061 gggagcgtac tcgcagtacg tgacccgggc gaatgagcgt agccgtcgat gaggcagtgt
    21121 gaaggatgaa gtgtaaataa aaaaggaagt gagcaatgcg taaaagcctg ttagctattc
    21181 ttgcagtctc ctcgttggta ttcagttctg cgtcgtttgc cgctgatctt gaagacaata
    21241 tggaaaccct caacgacaat ttaaaagtga tcgaaaaagc ggataacgcg gcgcaagtca
    21301 aagacgcgtt aacgaagatg cgcgccgcag cgctggatgc gcaaaaagca acgccgccga
    21361 agctcgaagg caaatcaccg gacagcccgg aaatgaaaga cttccgccac ggtttcgata
    21421 ttctggtcgg tcagattgac gacgcgctga agctggcaaa tgaaggtaaa gtaaaagaag
    21481 cgcaggctgc cgcagagcaa ctgaaaacga cccgcaacgc ctatcaccag aagtatcgtt
    21541 aattcctcat ttccctgttg cctgcactca ggtaacaggg aacttatcat cgcaaatgat
    21601 gcaccacctg attgctgctg ccgcgccaga tcagggaagg gtcttttaaa tcctgcacaa
    21661 atttgccgtc gaccagcacg ttaatcaaat caacaacctg catttgcgca gcgttgagtt
    21721 cgtcgagttt atagcctgtc cacacccaga tgtctttacc cggacactcg gcgcggatgc
    21781 gttgtaccag tttcagaata tccggcacgt tttgcggatg cagcggatcg ccgccggaga
    21841 gggaaatccc ctggcgtttg atacgagtgt cattcagatc gttaatgatc tggtcttcca
    21901 ttgctttggt aaatggctga ccagaattta cccgccatgt gcttttgtta tagcaaccgg
    21961 ggcattcatg aacacacccg gagacaaaca gggtgcagcg agtgccgggg ccgttgacga
    22021 tgtcgacagg atagtactga tgataattca ttatttcgcc tggcttatca gtccgcgtgt
    22081 aggctgcatc cggtaatcaa tgcctgatgc gacgctgtcg cgtcttatca ggcctacagc
    22141 cgttgccaaa tgtaggccgg ataaggcgtt tacgccgcat ccggcatttg cttagcgcag
    22201 agtgaagatt aacctatctg cccattcccc aaatgtttaa cgcggcgctt aacttcttcc
    22261 tgcttaccag cgttaaacgg acgtgcatcc gggctaccta aatatccgca cacgcggcga
    22321 gttaccgaca cacgggaggc gtcatggtta ccacatttcg ggcaagtgaa gcctttgctg
    22381 gtgcactcga actcaccggt aaagccacac tcgtagcact catcaatcgg tgtattggtg
    22441 ccgtaatacg gtacatgctg atagctgtaa tcccagacat cttccagcgc cttcaggttg
    22501 tgctgaatgt ttggatactc gccgtagcaa atgaaaccac cgttcgccag cggcgggtaa
    22561 ggcgcttcaa agtcgatctt gtcgtacggg ttcaccttct tctccacatc gaggtggaaa
    22621 ctgttggtgt agtaaccttt atcggtcacg cccggcacca cgccaaactc agcagtatcg
    22681 agacggcaga agcggtcgca caggttttca ctcggcgtgc tgtagagact gaaaccataa
    22741 cccgtttctt ctttccactg atccactgcc tgacgcagac gttcaacaat ggcgatacct
    22801 ttcgcgcgaa gctgctcgtt gtcatataca tgctcgccgc cgaacagcgc gttaatggtt
    22861 tcgtggatgc cgatgtaacc cagcgaaata gacgcacgac cgtttttgaa gatttcagaa
    22921 acatcatcgt cagcattcag acgcacgcca caagcacctt ccatatagag gatcggggcc
    22981 acgcgcgctt tcacgccttc gagacgagcg atacgggtca tcagcgcctt acgtgccagc
    23041 accagacgtt catccagcag cttccagaag gtggcttcat cgccttttgc ttccagagca
    23101 atacgcggca ggttcaggct gatcacgccg aggttgttac gaccatcgtg gatctgctcg
    23161 ccgttttcat tttcccacac gccgaggaag ctgcggcagc ccatcggggt tttaaacgaa
    23221 ccggtgactt tcactacctg atcgtagttc aggatatccg gatacatgcg cttgcttgcg
    23281 cactccagcg ccagctgttt gatgtcgtag ttcggatcgc cttttttatg gttcaggcca
    23341 tcgcgaatcg caaacaccag tttcgggaac accgcagttt tacggttttt accgaggcct
    23401 gcgatacggt tacgcaggat cgattcctga atcaggcgcg attcccagct ggtgcccagg
    23461 ccaaaaccaa aggttacaaa cggcgtctga ccgttggcgg tgtgcagggt gtttacttcg
    23521 tactccagcg actggaaggc atcgtagcac tctttaatgg tacgagagtt agcgtagcct
    23581 tcggcgtccg ggatgttcca ctcttctgcg gtcttgcgat gtttgttgta gctggcggtg
    23641 acaaacggtg ccagcacttc atcgatacgg ttaatggtgg tgccgccata aatatggctg
    23701 gcgacctgag caataatctg cgcagttacc gcggttgccg tagagatcga cttcggcggt
    23761 tcaatctcgg cgttccccat tttaaagccc tgggtcagca tgcctttcag gtcgatcaac
    23821 atgcagttga acatcgggaa gaacggcgag taatcgagat cgtgatagtg aatatcgcca
    23881 cgctcatgtg cctgcaccac gtcacgcggc agcaggtgct ggcgtgcata gtgtttagcc
    23941 acgatcccgg ccagcaggtc gcgctgggtt ggaatcacct tgctgtcttt gttggcgttt
    24001 tcgttgagta acgaggcgtt ggtctgctcg accagaccac ggatctcctg gttcaggcga
    24061 ccgcgttttt cacgttcaat gtcgcgatcg tgacggtact cgatgtaagc acgagccagt
    24121 tgtttgtatg gacccgacat cagctggttt tcaactgcgg tctggatctc gttgatatcc
    24181 acctggttgc ggccctgcat ctgctcgctg acaaccgcgg caacagtggc gcaataatcg
    24241 gcatcatcga cttccgctgc tttagctgca cgcagaatcg cttctttgat gcgctctgat
    24301 ttaaacggca ctttgcagcc gtctcgtttc atcacatgcg gtgtcatgat cgctccatat
    24361 ttttaagaac aggttatcca cagaaattgg gaaaggcgtt ttccggtttt ttcgtttcgt
    24421 tttccggtgc tttccgcaat cgccatccgc ttatccacaa cgccgcacct ttttcgtggg
    24481 gagctgtagt agcaattata gtcgattaat acaacatatt gggttgggac gcattttaaa
    24541 gtctatatat agtgctttgc atcaaggatg ttttaacttt ttttgatgta gctcaaagta
    24601 aaaagcagag cgtacggatg acgggcgcta cagcgttatg taaatttttt aatgaatttg
    24661 ctggttgaaa aatcaacaaa aacaacatgc tgacagacaa aaccccggaa tgaccggcat
    24721 taccggggct tagggaagat ttacttctgt aaccaccaga cagcctcaaa aggccgtaaa
    24781 ttcatggcac agggttgtgg tgaggcttct tcgtagttat gcatcacaag ctgccagttg
    24841 ccgcgcattt gccctggctg ccagggttgg atctcacggc taaggttggc aatgaccagc
    24901 aaggtttgcc ccttccattc acggcgatag caccacaata cagggctgtt tggcagcaga
    24961 tcctggtaat tgccccatgt caggatggct tcttgcttac gcagtgcgat taacttttgg
    25021 taggtgtaaa acaccgagga ctcatcggcc agcgcggctt ctacgttgat ttgttgatag
    25081 ttatcgccca ggccaatcca cggttcgcca gccgtaaacc cggcattatc gccgttgctc
    25141 cattgcatgg gcgtgcggct gttgtcacgg gatttactgg cgaggattgc caataactcg
    25201 tcggcatcac gaccatcgtt gcgcagctcg gcaaacatat ttaggctctc cacgtcgcga
    25261 tagtcagtaa tgcgcgtgaa atgcgggttg gtcatgccaa tctcttcgcc ctggtagata
    25321 tacggcgttc cctgcatgcc atgcagcacc atcgccagca tttttgccgc aggcacgcgg
    25381 tattcacctt catcaccaaa gcgagaaaca atgcgcggct gatcgtggtt acaccagaac
    25441 aaggcattcc atgccacgtt gtgcattcct tgttgccagt ggcggaacaa tgttttcaac
    25501 gccacaaagt caggtttagc cagcgtccat ttttcaccac cgggataatc gaccttcagg
    25561 tgatgaaaat taaaggtcat cgacaattca ctgcctgtca gtgccgcgta tcgctggcaa
    25621 tgctcaaggc tggtggagga catttcacct acggtcatta acccgcgtgg cgtaaacaca
    25681 tcgcggttca tctcgtgcaa aaactcgtgt gctcgtggcc cgtcggtgta gaagcgacgc
    25741 ccgtcgccat ccaggtcttc agggaaacgc gggtctttgg agatcagatt caccacatcc
    25801 aggcgcaacc cgtcgacccc acgatcggcc cagaattcac agactttttt cagctctgcg
    25861 cgtaccgctg gattctccca gttgagatcc gcctgttctg gtgcaaagag atgcaaatag
    25921 tactgttcgc tttccgcatg ccagcgccac gcactaccgc caaattttga acgccagttg
    25981 ttcggtggcg tttctggttc gccatcgcgc cagatataaa actggcggta agggctttct
    26041 ttgttcagcg cctcgcgaaa ccaggcatgt tgggtagagg tatggttaaa caccatatcg
    26101 agaatgatac gaatcccgcg cgattttgcc tgcgtcacca gttcgtcaaa atcgtccagc
    26161 gtgccgtagg tgggatcaat cgccgtatag ttcgctacat cgtaaccgtt atcgacctgg
    26221 ggagagacat aaaagggggt tagccagatg gcatcaacgc ccagtttatg cagatagtcc
    26281 aggcgttgga taacgccacg taaatcgccg gtgccgctac ccgtggtgtc ctgaaaactc
    26341 tttggataaa tctggtagat aacgccgttt tgccaccagt ggggaagatt agtcattacg
    26401 ttattcctgc aaatgcgagg gggcgcaatt gcgccccgaa gaaaattaaa caatgtccag
    26461 cgtgcccagg cggtatttcc gctgatagat aaacgaggtg agtacaatcg ggatgatgat
    26521 ggcgatagcc attgccagcg caaatacctg ccagtagctc ggttgaatcg agagaattcc
    26581 cggcaggccc cctacgccga taccattcgc cataacgccg ttcaggccgc atagtaatcc
    26641 tgccagacca gaaccaatca tcgcgcacag catcgggaag cgatatttca ggttgatgcc
    26701 gtacattgca ggctcagtga ccccaagcca ggcggagata gcggcaggca cggagatctc
    26761 gcgttcattg tgcttgcggc tggaaatgat aatgcctatc acggcggagc cctgagcgat
    26821 attcgacagc gcaatcagcg gccacactgg cgtgccaccc atgctttgaa tcatctgcaa
    26881 atcaatagca agcgtggtct ggtgtacacc ggtgatcacc agcggggcgt acaggaagcc
    26941 aaacaatgct gcgccaatcg gagcaaagct gccggtcatc aggtgacgta ccgcaaaggc
    27001 aacgccatcg ccaatcatgc gaccaaacgg accaatcagc gcatgggcga ggaacaccgc
    27061 gaggatcagc gaacagacgg gcaccaccac cagatagagg taatccggca cgatgcgttt
    27121 aaggcgagtt tcaataacgc ccagcgccag cccggctaac agtgccggga tcacctgcgc
    27181 ctgatagcct actttggcga tgctgaacat gccaaagtcc cacacttccg gcagctgctg
    27241 cccgagcaga taagcgttca tcagctgggg agaaaccagt gtcacgccaa gcacgatacc
    27301 aaggatcggc gtgccgccca tttttttcac cgctgaccag caaataccga ccggcaggta
    27361 gaagaagatc gcttcaccga tcaaccacag aaaatcgtag atcgtttgca gggaagggta
    27421 catttgcgcc agcgtctgac cgttgctcat gggcaaatcg ccgatcacat tgcgaaaacc
    27481 gaggatcaaa ccgccgctaa tcaacgcggg cagcagcggg aagaagatct ccgcgaaatg
    27541 agagatcaac tgctcatgcc atttcatatt ctgccgggcg gcttttttta cctgctcttt
    27601 atcaacctgc gcctgtccgg ttgacgcaat cagtgcttga tagtaatcac ccacattggt
    27661 gccaatcacc acctgaaatt gcccggcatt ggtgaaacag cctttcacca tggggagttg
    27721 ctcaatttct ttcggtctgg cattggccgg ttggttgagg acaaagcgca ggcgagtaat
    27781 acagtggctc accgtcgcaa tattgccgcg cccgccgacc agttcaatca accgatcgat
    27841 atccgtttgg tttattttgc tcatcataaa gccccatggc agatgacatt tttggttggc
    27901 tgcagaatat attgcgcgga aaaatttaaa aacgggaacg ttcccgaaac gcagcgaaga
    27961 tcacaattta tcgttcagga aacgatcagg acagggtggc ggggatgatg atttgttgcg
    28021 gttcgctgcg cccggttacc tgtgcgatca attggcaggc cgcctggcgt ccagcttcgg
    28081 cgtagccagg atctacggtg acgatctccg gatgaaggaa tttcattaac ggcgtattac
    28141 cgacgctcgc cagttgcaag gtgtcgatgc gttgctcttg caggtattta cttgcgccaa
    28201 gagccagagt gtcggttgcg cacagtaacg cggtagtttc aggcgtaatc acttttgcta
    28261 cgttttcata gccttgcttc atagcaagcc ctggcagagc ggcaacggga tgcagtttat
    28321 gcgctttgca gaacgccagg taggcttcgt gacgtcgctt cccggtcgtc acgtcactat
    28381 gcggcacgcc gagataactg atattgcgat gcccctggtc atagagccgt tgcatcagga
    28441 tcttgattgc cccttcgtcg tcataacaga ccgaagcaaa gccttttgcg tcacgcgcca
    28501 gcagaaccag cgatgactgc cagtgggcta acatttcttc tgttatgcca gtaaaaccga
    28561 acaacactac gccgtcgata ttacgccgtt tcagcacccc caaatgttcg gcaactaatt
    28621 gcggggaaaa ctgactttcc atcatgattg ggtcgtaacc ttgttcatag aacgctggca
    28681 gcatggtttg aacagcgaga ttttctgaca acgaatccag gcgggtaaca atgatggcga
    28741 ccactttatc gctttgccca cgcatagcgc gcgcagagcg ggaaggggaa aatccatgct
    28801 gattcatcac tgcttcaaca cgctcgcggg tgcgctggct cacgccgctt tcgttattca
    28861 gcacccggga aaccgtagat ttccccacgc cgcttaagcg cgcgatgtct ttgatggtca
    28921 gccgattttg catcctgttg tcctgtaacg tgttgtttaa ttatttgagc ctaactttac
    28981 ccgtgcattc agcaatgggt aaagtctggt ttatcgttgg tttagttgcc agcaggtatt
    29041 atatcgccat agatgctacg aatattattg gattctcctt attatttgcg gcgctttttt
    29101 cacttaccgg aggttatatg gaacctgatc ccacgcctct ccctcgacgg agattaaaac
    29161 ttttccggta agcccgtctt ttcacggcgt taccggatgc gtaaggccgt gacgttttaa
    29221 cgtccctgct cagctttatt accttcaggt aaggcttcgc cacgcctgaa gacatttctg
    29281 tactgtttca gacagtgcgg agggactcct tatgtttaaa gaaattttta cccggctcat
    29341 tcgccattta ccttcccgtc tggttcatcg cgatccattg cctggcgcgc agcagacagt
    29401 gaatacggcg gtcccgccgt ccttaagtgc gcattgcctg aaaatggcgg tgatgcccga
    29461 agaagaatta tggaaaacgt tcgacaccca tccggaaggg ttaaatcagg cggaagtgga
    29521 atctgcccgc gaacaacatg gtgaaaataa attacccgca caacaaccgt cgccgtggtg
    29581 ggtacattta tgggtctgct atcgcaaccc ctttaatatt ttactcacca ttctcggcgc
    29641 tatttcttac gccacggaag atttatttgc cgcgggcgtt attgcgctaa tggtcgctat
    29701 ttcaacgtta ctgaacttta ttcaggaagc acgttccact aaagcggcag atgccctgaa
    29761 agcgatggtc agcaataccg cgacggtgct gcgcgtaatt aacgacaaag gcgaaaatgg
    29821 ctggctggag atcccgatcg accagctggt gcccggcgat attataaaac tggcggcggg
    29881 agatatgatc ccggcagatt tacgtatctt gcaggcgcgg gatctgttcg tcgctcaggc
    29941 gtcgttaacc ggtgagtctc tgcccgtgga aaaagccgct accactcgcc agccggagca
    30001 cagcaatccg ctggagtgcg acacgctgtg ttttatgggc accaccgtgg tgagcggcac
    30061 ggcacaagca atggtgattg ctacaggtgc caatacctgg tttggtcaac tggcggggcg
    30121 tgttagtgag caggaaagcg agccgaatgc ctttcagcag gggatcagcc gcgtcagtat
    30181 gctgctgatt cgctttatgc tggtgatggc tccggtggtg ttgcttatca acggttacac
    30241 caaaggcgac tggtgggaag cggcactgtt tgcgctttcg gtcgcggtag gcttaacgcc
    30301 ggaaatgttg ccgatgattg ttacctcgac gctggcgcgc ggcgcagtaa agctgtcgaa
    30361 acagaaagtg atcgtcaaac atctggatgc tattcagaac tttggcgcaa tggatattct
    30421 gtgcactgat aaaactggca ccctgacaca ggacaaaatt gtgctggaga atcataccga
    30481 tatctccggt aaaaccagcg aacgcgtgct gcatagcgcg tggttgaaca gtcattatca
    30541 gaccggactt aaaaacctgc tcgatacagc ggtgctcgaa ggtacggatg aagagtcagc
    30601 gcgctcgctg gccagtcgtt ggcaaaaaat tgatgagatt ccgtttgatt tcgagcgtcg
    30661 ccggatgtcg gtggtagtgg cagaaaatac cgagcaccat cagctggttt gcaaaggtgc
    30721 attgcaggaa atcctcaatg tgtgttcgca ggtgcgtcac aatggcgaga ttgtgccgct
    30781 cgatgacatc atgctgcgta agattaagcg ggttactgat acgcttaatc gtcaggggct
    30841 gcgcgtggtt gcggtggcga cgaaatacct gccagcgcgt gaaggagatt accagcgggc
    30901 ggatgaatcc gacctgatcc tcgaaggata tattgctttc cttgatccgc caaaagagac
    30961 aaccgctccg gcactgaagg cattaaaagc gagtggaatc accgtaaaaa tccttactgg
    31021 cgacagtgag ttagttgcag cgaaagtgtg ccatgaagtg gggctggacg cgggagaggt
    31081 ggtcattggt agtgatattg aaacgctatc tgacgacgaa ctggcaaatc tcgcacagcg
    31141 caccacgctg tttgcccgcc tgacgccgat gcataaagaa cgcattgtga ccttactgaa
    31201 gcgcgaaggg catgtggttg gctttatggg cgatggtatt aatgatgcgc ccgccttacg
    31261 cgctgcggat atcggcattt ctgtggacgg cgcggtagat attgcccgtg aagcggctga
    31321 tatcatcctg ctggaaaaaa gcctgatggt gctggaagag ggggttattg aaggacgtcg
    31381 cactttcgcc aacatgctga aatacatcaa aatgacggcg agctccaact tcggtaatgt
    31441 gttcagcgtg ctggtggcga gtgctttctt gcccttcctg ccgatgttgc cgttacactt
    31501 gcttattcag aacctgctgt atgatgtgtc acaggtggcg atcccgtttg ataacgtcga
    31561 cgacgagcaa attcaaaagc cgcagcgttg gaatccggcg gatctggggc gctttatgat
    31621 cttcttcgga ccgatcagct cgatcttcga tattctgacg ttttgcctga tgtggtgggt
    31681 attccatgcc aacacgccgg aaacgcaaac gctgttccag tcgggatggt ttgtggtggg
    31741 cttactgtcg caaacgctga ttgtgcatat gatccgcacc cgccgtgtgc cgtttattca
    31801 gagctgtgca tcgtggccgt taatgatcat gaccgtgatc gtgatgattg tcgggatcgc
    31861 attgccgttt tcaccgctgg ccagttatct gcagctgcag gcgctgccgt taagctattt
    31921 cccgtggctg gttgcgattc tggcagggta tatgacatta actcagttgg tgaaaggatt
    31981 ttatagccgt cgttacggct ggcagtaaag aataaactgg gcacgatagc cccgattcag
    32041 attgcagaca aagtaagctt tgttcatgcc ggatgcggcg taaatgcctt atccggccaa
    32101 caaattcaat aaattgcagg aatcacgtag gcctgataag cgtagcgcat caggcgattt
    32161 tgcgtttgtc atcagactta atccgggcat gatagcccgg atttccatca agattagcga
    32221 cgaacagcga tcgcttcgat ctcaatcttc acgtctttcg gcagacgggc aacttcaacg
    32281 caagaacgtg ccgggaaggt ggcattgtgt tcggtgaaga aggcttcgta ggtagcatta
    32341 acggttgcga agtcgttcag atctttcaca aacacggtgg ttttaacgat gtcgcccact
    32401 ttcaggcctg cggcttcgac gatcgctttt acgttatcca gcgactgacg tgcctgtgca
    32461 gcgacgtctg ccggtacttc acccgttttc ggatttaccg ggatctgacc ggaggtgatg
    32521 atcatattgc ccagatcaac gccctgtacg taaggaccga tagctgccgg tgcattttcc
    32581 gtcgcgatag ttttgctcat gatttctcct ttattacagc ggtaaaaagt ctggctcatt
    32641 atagggagcc agacttttat taccaaccgc aattaattgg ccagcaccac attatgggaa
    32701 aactcttttt cacagtattt gcatttgagc gcgatgtcat tggcgcgttt tcgcacggca
    32761 aagctggaag aaaccggttc ggcatggctg atacagttgc tgttcgggca gaccagcaca
    32821 ttgtcgatgc gttctggcag actggggcgc gatttaccca ccacttcata gttgtcgata
    32881 cggttaaccg tggcttgcgg cgcatacaat gccagttgat ctacctgatc ttcactcaaa
    32941 aaggtatttt cgattttgat cagatctttg cggcccatct cgccagaagg caagttcaga
    33001 ccgatggtga tgcgctgatc cgtttcggtc agcttgaaca gactcaacag cttaaaaccg
    33061 atctgggcgg ggatatggtc aattaccgtg ccgcgtttaa tagcttcaac ctgcaattta
    33121 ttatcgtgtg tcatctctat ttccccttac agtaccagat cgcgattcag aaccagtgcc
    33181 agtaacgcct ggcgagcgaa aatcccgttg cctgcctgct ggaagtacca ggcgtgtggc
    33241 gttttatcaa catccgtcgc aatctcatca acacgcggca gcggatgcag cactttcata
    33301 ttggctttgg cgttgtggag atcgctggcg cgaagaacaa actgcgcttt cacgttggcg
    33361 tactcggacg ggtccagacg ctctttttgc acgcgggtca tgtacaggat gtctacttcc
    33421 gccatcactt cttcaataga gctgtgcaga ctccatgcga tccctttttc atcgagcata
    33481 tccagaatgt attgcggcat tgccagcgcg tccggcgcga tgaagtaaaa acggttgccg
    33541 tcgaacttcg ctaacgcctg agtcagggag tgaacggtgc ggccatattt caggtcacca
    33601 accattgcga cgtggagatt gtccagacgc ccctgggttt cctgaatagt gaataagtcc
    33661 agcaaggttt gcgtcggatg ttggttggag ccatcaccgg cattcagtac cggtacattg
    33721 ccggaaaact cggtggccag gcgcgccgca ccttcctgcg gatgacgcat cactatcgca
    33781 tcgacgtaag tgctgataac cgaaatggta tcggccagcg tttcgccctt tttacccagt
    33841 gatgtattgg cgctgtcgga gaagcccacc acgctggcac ccaggcggtg catagatgtt
    33901 tcgaaagaga ggcgggtacg ggtagaggct tcgaagaaac agctggcaat gactttgtgc
    33961 ttcaacagct ctggttgcgg gtttgctttc agtttcgccg ctgtcgccag caccagatta
    34021 aggtcatcgc gactaaggtc gtttatggaa atgatatgtt tctgatatag cggattagcc
    34081 atcttttatc tcctgacgcc tgggcaaaaa aaagcccctc gattgagggg ctgggaatgg
    34141 gtgatcaacg ggaagaaaaa cggcaggcca gcgtcttttt tcagacgcgg taagacaaaa
    34201 tgtcgaacac actgaaccat acatcctccc ggcaaattgt ccggcattat actcatcgtc
    34261 agaagcggcg caagcatttg atgcaatatt ttgtcagcgc aaacggttta tttgaattaa
    34321 aagtcaaggt atatgcattt ttatttgatg tgattctgca ggggaactgt cgttcggtat
    34381 caataattgc agacattccc tgcttttttc tttttcactt tcacgcaatc agatatgcat
    34441 tttattccat tcatccgact tatagggcgg agtgtgaaag cgaacggcta acactattgc
    34501 ttactgctca gggatgcgcg ctatcacttt aatttcaaaa tcaaagcctg ccagccatgt
    34561 aacacccacc gccgtccagt ttggataagg tggggcgcta aatatttcat ttttcaccgt
    34621 catgatgtct tcaaattggt tttctggatc ggtatggaag ctcgtaacat caatgatatc
    34681 gtcaaaagtg catcccgcag ctgccagggt cgcatgcaaa ttatcaaatg ccagtctgac
    34741 ttgttgctga aaatcgggtt ctggtgttcc gtcctctcga cttcctactt gcccggaaac
    34801 aaacagcaaa tcgccggaac gaatagccgc agaataacga tgctcagcat atagtgaatg
    34861 tcggccagca gggaaaacag cggttctttc taccatttgg ttatcctcaa gatttacgac
    34921 atgaacagaa gatttctctt taccgggagc cgcttttagc ggacgacgtg agtaaacaaa
    34981 acccagacat catggataat ggctgggctt aattgagcgt agtcggttat gcgccaaacg
    35041 cgccatcaat ggtatgcatc gcgccggtaa caaaactggc ttctggccct gctaaccatg
    35101 cgaccatacc agcgacctct tccggttgcc catgtctttt gatagccatc aaactatgca
    35161 acatatcgcg cattggcccg ttggcgggat tagcgtcggt atcaattggc cctggctgga
    35221 cgacgttaat ggtgatccca cgcggtccaa aatcacgggc cagcccgcgc gccatgcctt
    35281 gcagggcaga tttgctggcg gcataagcag ccatgcctgc aacaggcata cgatcgccat
    35341 tcacggagcc gatgattaag atgcgcccgc cttcgggcat ctgccgggcg gcttcaacag
    35401 aggcatgata aggagcatga atattgattt tgaaaaggcg atcaatatcg tcggcattta
    35461 attccagggc ctcgccaaag acgccaatac ctgcatttac caccaggata tccaatgcgc
    35521 cgctcttacg aacgacatca atgacagcgt ctctgtcagc actatctgtg aatactgctg
    35581 tcgctccagt ctcttgtgcc aggcgtttag cggcatcttt cgaccccgca taggtgaatc
    35641 gtacattggc cccatcggtg acgaaacgac gtacgatagc ggcaccgata ccacgactgc
    35701 caccgaggat gagaactgtc ttacctgtaa aagcgcccat aaggactcct tgatttatta
    35761 tgtaacatgc attacaaaac tgttttaact ttctgtcaac aggttttgta atggtcacta
    35821 aaaaacaatc tcgcgttcca ggtcgtccca gacgtttcgc tcctgagcag gcaatctctg
    35881 cggcaaaagt gctttttcac caaaaaggtt tcgatgctgt cagtgttgct gaagttactg
    35941 attatcttgg tattaacccc ccgagcctct acgcggcttt tggcagtaaa gctgggttat
    36001 ttagccgtgt actcaatgaa tacgtcggta cggaagctat tccgcttgcc gatattcttc
    36061 gtgatgatcg tccagtaggc gagtgcctgg ttgaggtatt aaaagaagcg gcgcgcagat
    36121 atagccaaaa cggcggctgc gctggctgta tggttcttga aggtattcat agtcatgatc
    36181 cactagcgcg tgatattgcc gttcaatatt atcacgccgc agaaacgacc atttatgact
    36241 atatcgccag gcggcatcca caaagcgcac aatgtgtgac tgattttatg agtaccgtga
    36301 tgtcagggct ttctgcgaag gcacgggagg ggcactcaat cgaacaactc tgtgcaacag
    36361 ctgcactggc gggggaagcg ataaaaactc ttctcaagga gtgatgctgg ccttgatccg
    36421 aaaggcggga acgcgcctgc cgataagttg tgataagaca ataattcacg cgttaaggct
    36481 agcggaattg atcatctttt cgtataacga tagaaatgaa acgttgtttt aattaaggag
    36541 tggaaaagat gatcatcgga aatattcata atcttcagcc gtggctaccc caggagttac
    36601 gccaggcgat tgagcatatc aaagcacacg ttacggcaga aacgccaaag ggcaagcacg
    36661 atatcgaagg caatcgtctg ttttatctta tctcggaaga tatgaccgag ccgtacgaag
    36721 ctcgccgtgc ggagtatcat gcccgctatc tcgacattca gattgtgtta aaaggtcagg
    36781 aaggcatgac cttcagcacg caacctgcag gcacgccgga taccgactgg ttagctgata
    36841 aagacatcgc atttttgccg gaaggcgttg atgagaaaac agttatcctt aatgaaggtg
    36901 attttgttgt gttttatccg ggggaagtgc ataaaccgct gtgcgcagtg ggcgcgccag
    36961 cccaggttcg caaagcagta gtgaagatgc tgatggcgtg atgacttttc gccgtaaata
    37021 actccaggtt tacggcgagt ttgtgaaaag agcgtttttt gatatttttt tgtgagtaga
    37081 atttgtaatg cttagacgtt cttattaact caaggagtcc gtcatgagca aaatatcagg
    37141 ttggaatttt tctcaaaaca ttacatcagc cgacaattgt aaacaaaaaa atgaagactt
    37201 agatacctgg tatgtgggaa tgaatgattt tgcccgaatt gccggagggc agaatagcag
    37261 aagcaatatt ctttttccca gagcattttt ggagtttttg gctaagatat ttaccctggg
    37321 ttatgtggat tttagcaaac gctccaacga agcgggtaga aatatgatgg ctcatattaa
    37381 gtcctcatct tatatcaaaa ataatgatgg tagtgagata atgcagtttg ttatgaataa
    37441 tcctgaaggg gagcgagcgg atttatctaa ggtggaaata gaaattacac ttgcctcagc
    37501 ttttaataat gggattcgtg aaggacatac agtcattata tttacacaac ctgatggttc
    37561 gactaatcgt tatgaaggga agtcctttga aagaaaagat gagagttcat tacacctgat
    37621 tactaacaag attctggcgt gttaccaacg cgaagctaac aaggaaatag ctcgtctatt
    37681 aaatattccg caggagttaa ataattctca ggacttgaat aactcgcagg tgagttgtaa
    37741 agattcagtt gattctacga ttacggattt attagaaaaa ccattgaata atgcattatt
    37801 agcaataagg aaagaacatc tgctattaat gccttatgta tgtaatgaat cgatttcata
    37861 cttgcttggt gaaaaaggga tactgaaaga aatagatga
//
LOCUS       DAPGOF010000024        37825 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.024, whole genome shotgun sequence.
ACCESSION   DAPGOF010000024 DAPGOF010000000
VERSION     DAPGOF010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.024"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            64..2307
                     /gene="pdeF"
                     /locus_tag="RHV13_001086"
     CDS             64..2307
                     /gene="pdeF"
                     /locus_tag="RHV13_001086"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HDV1240641.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDVKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELISPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMADNRAKMPAHRFAINLSPTSVCQARFPVEVSQLLDKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEN
                     EPIRESA"
     gene            complement(2346..3887)
                     /gene="ppx"
                     /locus_tag="RHV13_001087"
     CDS             complement(2346..3887)
                     /gene="ppx"
                     /locus_tag="RHV13_001087"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HDV1240642.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(3892..5958)
                     /gene="ppk1"
                     /locus_tag="RHV13_001088"
     CDS             complement(3892..5958)
                     /gene="ppk1"
                     /locus_tag="RHV13_001088"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HDV1240643.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6130..6768)
                     /gene="purN"
                     /locus_tag="RHV13_001089"
     CDS             complement(6130..6768)
                     /gene="purN"
                     /locus_tag="RHV13_001089"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDV1240644.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDVTARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(6768..7805)
                     /gene="purM"
                     /locus_tag="RHV13_001090"
     CDS             complement(6768..7805)
                     /gene="purM"
                     /locus_tag="RHV13_001090"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDV1240645.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8130..8756
                     /gene="upp"
                     /locus_tag="RHV13_001091"
     CDS             8130..8756
                     /gene="upp"
                     /locus_tag="RHV13_001091"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDV1240646.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            8842..10131
                     /gene="uraA"
                     /locus_tag="RHV13_001092"
     CDS             8842..10131
                     /gene="uraA"
                     /locus_tag="RHV13_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDV1240647.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            10181..10927
                     /gene="hda"
                     /locus_tag="RHV13_001093"
     CDS             10181..10927
                     /gene="hda"
                     /locus_tag="RHV13_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HDV1240648.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(11065..11424)
                     /gene="arsC"
                     /locus_tag="RHV13_001094"
     CDS             complement(11065..11424)
                     /gene="arsC"
                     /locus_tag="RHV13_001094"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HDV1240649.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(11445..12908)
                     /gene="bepA"
                     /locus_tag="RHV13_001095"
     CDS             complement(11445..12908)
                     /gene="bepA"
                     /locus_tag="RHV13_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HDV1240650.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANSYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13121..14182
                     /locus_tag="RHV13_001096"
     CDS             13121..14182
                     /locus_tag="RHV13_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDV1240651.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(14220..15068)
                     /locus_tag="RHV13_001097"
     CDS             complement(14220..15068)
                     /locus_tag="RHV13_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
                     /protein_id="HDV1240652.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSICYRAI
                     YLRQES"
     gene            complement(15090..17102)
                     /gene="hyfR"
                     /locus_tag="RHV13_001098"
     CDS             complement(15090..17102)
                     /gene="hyfR"
                     /locus_tag="RHV13_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HDV1240653.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATAEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLTRIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLIQTLQTRQPTLTHRADDLFLWQRDPLLHLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQKSLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRAIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(17132..17545)
                     /gene="hyfJ"
                     /locus_tag="RHV13_001099"
     CDS             complement(17132..17545)
                     /gene="hyfJ"
                     /locus_tag="RHV13_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HDV1240654.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYKDWLALVEEEQARRKMLGVMTFGEIVIDVSHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(17538..18296)
                     /gene="hyfI"
                     /locus_tag="RHV13_001100"
     CDS             complement(17538..18296)
                     /gene="hyfI"
                     /locus_tag="RHV13_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HDV1240655.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKLHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(18293..18838)
                     /gene="hyfH"
                     /locus_tag="RHV13_001101"
     CDS             complement(18293..18838)
                     /gene="hyfH"
                     /locus_tag="RHV13_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HDV1240656.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            complement(18848..20563)
                     /gene="hyfG"
                     /locus_tag="RHV13_001102"
     CDS             complement(18848..20563)
                     /gene="hyfG"
                     /locus_tag="RHV13_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HDV1240657.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYPFINEGNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(20553..22133)
                     /gene="hyfF"
                     /locus_tag="RHV13_001103"
     CDS             complement(20553..22133)
                     /gene="hyfF"
                     /locus_tag="RHV13_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HDV1240658.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGHSATRAVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     EHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(22138..22788)
                     /gene="hyfE"
                     /locus_tag="RHV13_001104"
     CDS             complement(22138..22788)
                     /gene="hyfE"
                     /locus_tag="RHV13_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HDV1240659.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNNPQNIPEKALFGPAMMTLLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(22800..24239)
                     /gene="hyfD"
                     /locus_tag="RHV13_001105"
     CDS             complement(22800..24239)
                     /gene="hyfD"
                     /locus_tag="RHV13_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HDV1240660.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFALSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVTGALSYSCGTRLLPLLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            complement(24256..25200)
                     /gene="hyfC"
                     /locus_tag="RHV13_001106"
     CDS             complement(24256..25200)
                     /gene="hyfC"
                     /locus_tag="RHV13_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HDV1240661.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLLTPLFTGISRQIRARMHSRRGP
                     GIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGGG
                     DLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIAG
                     STHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTEY
                     SGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFVL
                     ASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(25211..27229)
                     /gene="hyfB"
                     /locus_tag="RHV13_001107"
     CDS             complement(25211..27229)
                     /gene="hyfB"
                     /locus_tag="RHV13_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HDV1240662.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFDFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNLFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTQVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(27229..27846)
                     /gene="hyfA"
                     /locus_tag="RHV13_001108"
     CDS             complement(27229..27846)
                     /gene="hyfA"
                     /locus_tag="RHV13_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HDV1240663.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENAPTQHALLRWEPGVQTVAVKCDLCDFLPQGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(28099..28569)
                     /gene="bcp"
                     /locus_tag="RHV13_001109"
     CDS             complement(28099..28569)
                     /gene="bcp"
                     /locus_tag="RHV13_001109"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HDV1240664.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(28569..29141)
                     /locus_tag="RHV13_001110"
     CDS             complement(28569..29141)
                     /locus_tag="RHV13_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HDV1240665.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDTHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            29287..30165
                     /gene="dapA"
                     /locus_tag="RHV13_001111"
     CDS             29287..30165
                     /gene="dapA"
                     /locus_tag="RHV13_001111"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDV1240666.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            30182..31216
                     /gene="bamC"
                     /locus_tag="RHV13_001112"
     CDS             30182..31216
                     /gene="bamC"
                     /locus_tag="RHV13_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HDV1240667.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            31429..32142
                     /gene="purC"
                     /locus_tag="RHV13_001113"
     CDS             31429..32142
                     /gene="purC"
                     /locus_tag="RHV13_001113"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDV1240668.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            32310..33173
                     /locus_tag="RHV13_001114"
     CDS             32310..33173
                     /locus_tag="RHV13_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HDV1240669.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            33188..35203
                     /gene="tmcA"
                     /locus_tag="RHV13_001115"
     CDS             33188..35203
                     /gene="tmcA"
                     /locus_tag="RHV13_001115"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HDV1240670.1"
                     /translation="MAELTALHTLTAQMKREGNRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHFKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPSGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSNAL
                     VFDDENFTHTPQGNIVISAFEQTLWRIEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGVGQQLIASALQYRPGLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDIVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            35277..35975
                     /gene="ypfH"
                     /locus_tag="RHV13_001116"
     CDS             35277..35975
                     /gene="ypfH"
                     /locus_tag="RHV13_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDV1240671.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(36095..36295)
                     /locus_tag="RHV13_001117"
     CDS             complement(36095..36295)
                     /locus_tag="RHV13_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HDV1240672.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(36323..37450)
                     /gene="dapE"
                     /locus_tag="RHV13_001118"
     CDS             complement(36323..37450)
                     /gene="dapE"
                     /locus_tag="RHV13_001118"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HDV1240673.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(37454..37810)
                     /locus_tag="RHV13_001119"
     CDS             complement(37454..37810)
                     /locus_tag="RHV13_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HDV1240674.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT         9163 a         9795 c         9991 g         8876 t
ORIGIN      
        1 gtcaggtggg atgcctgtct gaatacacct ccttcaggat gtgggggatt ctgctgagca
       61 tctatgaaac tgaatgcaac ttatataaaa atacgtgata aatggtgggg gcttccgctg
      121 ttcctgcctt ctttaatctt gcccattttc gcccacatta atactttcgc gcatatttct
      181 tccggtgagg tttttctctt ttatctgcct ctggcactga tgatcagcat gatgatgttt
      241 ttcagctggg cggcattgcc agggatcgcc ttagggattt ttgtccgcaa atatgcagag
      301 cttggttttt acgaaacgct ctcattaacg gctaatttta ttatcattat cattctctgt
      361 tggggcggtt acagggtctt tactccccgg cgtaacaacg tttcacatgg tgatacccgt
      421 ttaatttccc agcgtatatt ctggcagatt gtgtttcctg caacgctgtt tctgatactt
      481 ttccagtttg ctgcgtttgt aggattactg gcgagcagag aaaatctggt cggcgtcatg
      541 ccttttaacc tcgggacctt aatcaattat caggccttac tggtgggcaa tctgattggt
      601 gtcccgctgt gctacttcat cattcgggta gttcgaaatc cgttttattt acgtagttat
      661 tattcgcaat taaaacagca ggttgatgtc aaagtcacca aaaaagagtt cgcactctgg
      721 ctactggcat taggtgcttt actgttgctg ttatgcatgc cgttaaatga aaaaagcaca
      781 atttttagca ccaattatac cttgtcatta ttgctgcccc tgatgatgtg gggagcgatg
      841 cgctatggtt ataagctgat ttcactgctc tgggcggtcg tgttgatgat cagcatccac
      901 agctatcaaa attacattcc catttatcct ggctatacca cgcagctgac cataacctcc
      961 tccagttatc tggtattctc ttttattgtc aattatatgg ctgtactggc aacccgtcag
     1021 agagcggtag tcagacgcat tcaacggctt gcgtatgtgg acccggtggt tcatctgcca
     1081 aatgttcgcg ccctgaatcg cgcgttgcgt gatgccccct ggtctgcgct ttgttattta
     1141 cgcatccctg gcatggaaat gctggttaaa aactatggca tcatgctgcg gattcaatac
     1201 aagcaaaaac tttctcactg gctgtcacca ttgctggaac cgggtgaaga tgtttatcag
     1261 ctttcgggta acgatctcgc gctgcgactg aatacagaat cgcaccagga gcgcattacc
     1321 gcactggata gccatctcaa gcaatttcgt ttcttttggg atggcatgcc gatgcaaccg
     1381 cagattggcg tcagttactg ctatgtgcgc tcgccagtga atcatatcta cctgctgctg
     1441 ggagagctaa atacggtcgc cgaactttcc atcgtgacca acgccccgga aaatatgcag
     1501 cgtcgcgggg caatgtattt gcaacgcgaa ttgaaagata aagtcgcgat gatgaatcga
     1561 ctacagcagg cgctggaaca caaccatttt ttcctgatgg cccagccgat taccggtatg
     1621 cgtggtgatg tttaccatga aattcttctg cgcatgaaag gtgagaatga tgaactgatc
     1681 agccccgata gcttcttgcc ggtcgcgcac gaatttggtt tatcgtcgag tatcgacatg
     1741 tgggtcattg agcatacgct gcaatttatg gctgataaca gagcgaagat gcccgctcac
     1801 cgttttgcta ttaatctgtc tccaacctcg gtatgtcagg ctcgttttcc tgttgaagtc
     1861 agtcagttgc tggataaata tcagattgaa gcgtggcaac ttatttttga agtcaccgaa
     1921 agtaatgctc tgaccaatgt taagcaggcg caaatcacct tgcagcatct tcaggaatta
     1981 ggctgccaga ttgcgattga tgatttcggc accggctacg ccagctatgc gcggcttaaa
     2041 aatgtgaatg ccgatctgct taaaattgac ggcagtttta tccgcaatat tgtgtcaaat
     2101 agtctggatt atcagatagt ggcatcgatt tgccacctgg cgcgaatgaa gaaaatgcgg
     2161 gtagtggcag agtacgttga aaacgaagag atccgcgagg cggtgctctc tttggggatc
     2221 gattatatgc agggttatct tattggtaag ccgcagccgt taattgatac gttgaatgaa
     2281 aacgaaccca ttcgcgaaag tgcctgaata atgcgggccg acatttctcg tcggcccgca
     2341 aagtattaag cggcgatttc aggtgtactt tcttcttcaa ttttcaaccg ccagccagcc
     2401 acgccttccc agtattcttg ctccttttcc agatcaagca gtaccagcgc attctgacta
     2461 aaccagtcat gcgggaaacg cagtgtccag tgactgtcat cagtaatcag cgtcaatgtt
     2521 ggcggtgtgg tggttgcctg acgttgattg ttgagtaata cgccaaggcg caatagctga
     2581 atcagtggca ggaactgttt cttcttaaac aaggtaaagc gcggcagatc gtcgagttta
     2641 atcgctttac ggtgatagcg caccagtgtc gccatcatca gctgctgttc ctgattaaaa
     2701 cccggcaagt cactgttttg cagaatataa gcggagtggc gatgcaaacc gctgtggttg
     2761 atattcaacc cgacctcatg cagcatggcg gcccatcgca gtagcgcctc cagttgcgga
     2821 tgcgccagct tcggttgctg ttcccgccac tgttcgtaca tttgcatagt ggtatccagc
     2881 actcgtcggg cctgttcgct gtctatgtga tactggttgg cgaggctgct ggcggtgcga
     2941 ctacgcacat cctgatgacg gaaacgtcct tccatttcat acagtacgcc ttcgcgtaac
     3001 gccccgtcag aaaggcgcag ttcacggatg gctaaagcat caaacacacc acataaaatc
     3061 gccagtcctg gaacgaagac tgttttccgc tcttcggaaa gacctggtaa actcagcgat
     3121 gcgaaattac ggtgccgtaa aacttctttt accagttttt ccagacgttc cggggtaatt
     3181 atcccgtctt tctcgcccat ttccattaac acttcatggg cggcttttat ggtaccggaa
     3241 gcgcccattg ccacgttcca gccctgaata cgaaattgcc aggttaaagt ttccagtttt
     3301 tgtgctgccg ccatgcgagc gcgctgaaaa ttctctttat tgatgacccc gccagggaaa
     3361 taaagctggg caaagctgac acaacccatc cggcggcttt caacgagaat cggttcgaaa
     3421 ttttcaccaa tcaccagttc cgtagatccg ccgccaatat caataaccag tttgcgacct
     3481 ttttccggtt gggtatgttc cacgcccata aaaatcagac gggcttcttc attaccggaa
     3541 ataatttcaa tcgggtaggg aatgaccttt tccgcacgtt tcagaaagtc agtggcgttc
     3601 agcgcctgac gcagcgtatg ggtaccaact atacagacgc tggcaggaga aaacccttgt
     3661 agccgttcgg caaacagcga cagacagttt aaaccgcgcg tcattgcctc ttcactcaac
     3721 atattatctg gccccaggcc gtccgccaga tgcacccgct gtttcaggcg gccaataatc
     3781 tgcatggcac catctaccac acgggctatg accatgtgaa aactgtttga accaagatcg
     3841 accgcagcaa actcctgcgg acgaggggat ttatcgtgta ttggcatagg gttattcagg
     3901 ttgttcgagt gatttgatgt agtcgtaaat cgccaactgc gcccgtactt tgcggcgatt
     3961 gccgcgggga acgtagcgat tactgagttc tttatcgata taacgtgctt tgaccgtatc
     4021 gctgaacaat atgtcgatga tgtccagcac ccgctgcttc aggcgcggat cgagcagcgg
     4081 cgtcgccact tcaatacgat aatcaatatt gcgcgtcatc cagtcggcgg aagaaaggta
     4141 gaccttttta tcgccgccat tttcaaaaat ataaacccgg tcatgttcaa ggtaacggtc
     4201 aacaatactg atggcacgaa tgttgtcgct aatgccttcc agattgggga tcagcgaaca
     4261 cattccgcga accagcagat taaccggtac gccggagctg gaggccgcat acagacgatc
     4321 aaccaggcct ttatcgacaa ggttatttag cttcagggtg ataccgctgg gcagcccttg
     4381 ctgcgcgttg gcgatctcgc ggtccaccat ttcatacaac aggcggcggg agttttgcgg
     4441 cgacaccatt aaataatcaa atgtcaccgg acggtatggg ttttcaataa agttaaatac
     4501 ccgccgtact tcgttggtga tgcgcgcatc ggcggtcagc aacgaatagt cagtataaag
     4561 acgcgcggtt ttttcgttaa agttcccggt cccgatgtgt gcataacgca ccacttcacc
     4621 gttttcttta cgtgaaatca ggaacagttt ggcgtgaatt ttcagccccg gcgcagagaa
     4681 gataacgtgc acgcctgctt cggtcaggcg cttcgcccag tgaatgttgg cttcttcgtc
     4741 gaaacgcgcc tgtaactcaa ccaccacggt gactttctta ccattgtgtg cagcgtggat
     4801 catcgagtcg atgatgcgtg aatctttcgc cacgcggtaa atgttaattt taatcgccag
     4861 tacgctcggg tcgaacgaag cctgacgcag cagttccagc acatgctcaa aggtatgata
     4921 aggataatag agcaacacat cgcgttcgcg aatggcatca aaaccattgc ggaactgggc
     4981 tttatcaaac caaatatggc gtaaacgcgg cagtggtttg ttcaccagat tggctttgcc
     5041 gacattgggg aaattaataa agtctttaaa attatgataa cgaccgccgg ggacgatgga
     5101 gtcgtagcgg gaaatagtca gtttttcgcg taacacttca accagcgcat tgggcatatc
     5161 gcgctgataa acaaaacgca ccggctcagc agttaaacgc tgcttgagac tggaagacat
     5221 caactccatc aggctggctt ccatctcatg cactaaatcg tattcggcat cgcgggtcat
     5281 cttcattgaa taggcattca gcgcgtcata atcaaagaag cctttgaaaa tatcatcaag
     5341 gcagtaacgc agaatgttat ccagaagaat catcggcttg cgtcgacgcg gtgcttctgg
     5401 cggtaaattc acaaagcgcg gcactttatc tgatgggatc tccagcagcg cgtaacggat
     5461 ggtatcgcca cggataattt ccaccgccag ataggtgtaa tcatctttca ggaactgcac
     5521 taagtcagtg tcaggattga ttaaaatcgg cgtaatgtgc tgacgcagat actgcttaaa
     5581 ataatgacgc agccagtttt gttgattgac ggagagctgg cgttcattaa tcaggaagat
     5641 ctggttacgc gccatctcca gcagcagctc gttgtagagg ccgtcgaatt cctgatcggc
     5701 tttcagcacc cgggactgaa ttttgcccag taaatggcgg gaatgagagt tggagccttg
     5761 ttcttcgcta ataatgatgc gtcgcttcag ttcagcgaag cggactttat agaactcatc
     5821 aaggttattg gaatagatcc ccaggaaacg catcctttca atcagcgggt tagatttgtc
     5881 cgccgcttcc tgaagcacgc gttcattgaa cgataaccaa ctgagctctt tttcgatgta
     5941 tagcttttcc tgacccatta ccacttttac tccgttttat tcacgggaca ctgcctggat
     6001 attatggcga gcaatattgg cagatgtcca actatgccag aaaaggggga aaaaaactgc
     6061 tggagcttgc gatttacagg tgaattgaaa aacgccagcg gcagagctgg cgctttaact
     6121 acgggggcat tactcgtcgg cagcgtagcc ctgcggcggc agacgttgac catccagcca
     6181 cgcggcgttt tcgtgcattt tcagacgacc atcggcaaac cagctaatca ccagtggata
     6241 aatagcgtgt tcctgggttt gcacgcgggc ggtgacgtca tcttccgtat caccagcaaa
     6301 taccgggact ttcgcctgta aaataaccgg gccaccgtcc agttcatcgg tgacgaaatg
     6361 caccgatgta ccgtgctctt catcgccatt ttccagcgcc tgacgatggg tgtgtaatcc
     6421 gggatatttc ggcagcagag aagggtgaat gttcagcaaa cgcccggcat agtgggagac
     6481 aaacgccggg ctgagaatgc gcataaaacc agccagcacg accacatcgg gtgcgtacat
     6541 gtcgatttca tgaatcaact cccggtcata ggcttcacga ctgtcaaacg cgctggcgat
     6601 gagcgtatgc gttgcaatac ccgcctggcg ggcgcgttca aggccgaacg cgtcggcctt
     6661 attgctgaaa actgcccgta cggtgccttt aattttgttg gttttacagg cgtcaataat
     6721 tgcctgtaaa ttacttccgt taccggaaat aagcaccaca atattcatta ttcgataacc
     6781 acgcgttgtt cggaatcaga ggctttgatg ataccgattt tccacgcgtt ttcaccgttg
     6841 gcattgagca gggcgagggc tttgtccact tccggagcag gcagggcaat aatcatcccg
     6901 acgccgcagt tgaaggtgcg atacatttca tggcgctcaa cgttacctgc cgtttgcagc
     6961 cagttgaaca cttccggcca ctgccaggaa gattcatcaa tcactgcctg agtattatct
     7021 ggcaatacgc gtggaatgtt ttcccagaag ccgccgccgg tcaggtgcgc aatggcatgc
     7081 acatcgacct tttcaatcaa ctccagcact gacttcacgt aaatgcgggt cggtgccagc
     7141 agatgatcgg ctaatggctt accatcaagt tcggtggttt gcggatcaca accgctgact
     7201 tcaagaattt tgcgcaccag cgaatagccg ttcgagtgcg gaccgctgga accgagtgca
     7261 atcagaacat cgccgtcgct gactttagag ccgtcgatga tttctgattt ttctaccacg
     7321 cccacgcaga aacccgcgac atcgtaatct tcaccgtgat acatccccgg catttctgcc
     7381 gtttcgccac ccaccagtga acagcccgat tgcagacaac cttccgcgat gccgctgatc
     7441 accgctgaag cggtatcaac atccagtttt ccggttgcgt aatagtcgag gaaaaacagc
     7501 ggttctgcac cttgcaccac caggtcatta acgcacatgg cgaccagatc aataccaatg
     7561 gtgtcgtgac gttttaagtc cattgccaga cgcagcttgg tacctacgcc gtcagtgccg
     7621 gaaaccagca cgggttcacg atatttttgc ggcaatgcac acagcgcacc gaagccgccc
     7681 agaccgccca tcacttccgg acgacgcgtt ttcttcacta cgcctttgat tcttccaacc
     7741 agagcattac ccgcgtcaat atcaacaccg gcatctttgt agctaagaga ggttttatcg
     7801 gtcactgctt gggtccccac gcgttacttg cggtagaaaa ataaaattcg gcgcaattct
     7861 aacagggaaa gcaaacgttt gcgagactgc tttacacaac ctttttgcac gtcttttccc
     7921 caggcgcgcg gcgaaagaag acttgtgcca gggcaaaggt tagttttcgg atggaataat
     7981 cttctttcat aaccatttga atataaaata actttatctc aaaccgttat cattttgact
     8041 aaagtcaacg aaaataatat tgccgccttg aagaaaggag gtataatccg tcgatttttt
     8101 ttgtggctgc ccctcaaagg agaaagagta tgaagatcgt ggaagtcaaa cacccactcg
     8161 tcaaacacaa gctgggactg atgcgtgagc aagatatcag caccaagcgc tttcgcgaac
     8221 tcgcttccga agtgggtagc ctgctgactt acgaagcgac cgccgacctc gaaacggaaa
     8281 aagtaactat cgaaggctgg aacggcccgg tagaaatcga ccagatcaaa ggtaagaaaa
     8341 ttaccgttgt gccaattctg cgtgcgggtc tggggatgat ggacggtgtg ctggaaaacg
     8401 ttccgagcgc gcgcatcagc gttgtcggta tgtaccgtaa tgaagaaacg ctggagccgg
     8461 taccgtactt ccagaaactg gtttctaaca tcgatgagcg tatggcgctg atcgttgacc
     8521 caatgctggc aaccggtggt tccgttatcg cgaccatcga cctgctgaaa aaagcaggct
     8581 gcagcagcat caaagttctg gtgctggtag ctgcaccaga aggtatcgct gcgctggaaa
     8641 aagcgcatcc ggacgtcgaa ctgtatactg catcgattga ccagggactg aacgagcacg
     8701 gatacattat tccgggcctc ggcgatgccg gtgataaaat ctttggtacg aaataaagaa
     8761 taaaaataat taaagccgac tttatgagtc ggcttttttt tgagtaaagc gcctataaca
     8821 cataatacag aggataatac tatgacgcgc cgtgctatcg gggtgagtga aagaccgcca
     8881 cttttacaga caatcccgct tagtttgcaa catttgttcg ccatgtttgg tgcaaccgtc
     8941 ctggtgcccg tcttatttca tattaacccg gcgactgtac tgttatttaa cggtattgga
     9001 acgctgctgt atctcttcat ctgtaaaggg aaaattccgg cttatcttgg ttccagcttt
     9061 gcctttattt caccggtatt gttactgttg ccgttagggt atgaagtcgc gctgggcggc
     9121 tttattatgt gcggcgtgct gttctgcctg gtttctttta tcgtgaagaa agcggggacc
     9181 ggctggctgg acgtgctgtt tccacctgcg gcaatgggcg caatcgttgc cgtcatcggt
     9241 ctggagctgg cgggcgtagc tgccggtatg gcgggtttac tcccggctga agggcaaacg
     9301 ccagactcca aaaccatcat catctctatt accaccctgg cggtcacggt tttaggttcc
     9361 gtgctgttcc gtggtttcct ggcaattatc ccgattttaa ttggcgtgct ggtggggtac
     9421 gcgctctctt tcgcaatggg aattgtcgat accacgccga ttattaatgc tcactggttt
     9481 gcgctgccaa ccctctatac gccgcgcttc gagtggtttg ctattctgac tattctgcca
     9541 gcggcgttag tggttattgc cgaacacgta gggcacctgg tagtaacggc taatatcgtc
     9601 aaaaaagatc tgctgcgcga tccaggtctg caccgttcga tgtttgctaa cggcttgtcg
     9661 accgtgattt ccggcttctt tggctccacg ccaaacacca cctacgggga aaacattggg
     9721 gtgatggcga tcacccgtgt ttacagtacc tgggttatcg gcggggcggc gattttcgct
     9781 atcctgcttt cctgcgtcgg taaactggct gctgctatcc agatgatccc attgccggtg
     9841 atgggtggtg tttcgctgct gctttacggt gtcatcggtg cttccggtat tcgtgtgttg
     9901 atcgaatcga aagtggatta caacaaagca cagaacctga tcctgacttc cgtgattttg
     9961 atcatcggcg tcagtggcgc gaaggtaaac attggcgcgg cggagctgaa aggtatggcg
    10021 ctggcgacca tcgtcggtat cggtttaagt ttgattttca aactgatcag tgtgctgcgc
    10081 cctgaagaag tggtgctgga cgcagaagac gcggatataa cagacaaata atgtaccgac
    10141 cgggcagtgt tgctgcccgg ttcaaacatc atgggattct gtggtaaact tctcgcgatt
    10201 ttgtgaaatc ctggttgagg tatctctgaa cacaccggca cagctctctt tgccacttta
    10261 tcttcctgac gacgaaacct ttgcaagttt ctggccgggg gataactcct ctttactggc
    10321 cgcgctgcaa aacgtgctgc gtcaggaaca tagcggttac atctatctct gggcacgcga
    10381 aggcgcgggg cgcagccatc tgctgcacgc ggcttgcgcg gaattgtcgc agcgtggcga
    10441 tgcggtgggc tatgtcccgc tggataaacg cacctggttt gttccggaag tgctcgacgg
    10501 tatggagcat ttgtcgctgg tctgtatcga caacattgag tgtattgcag gcgatgagtt
    10561 gtgggagatg gcgattttcg atctctacaa tcgaattctg gaatcgggca aaacacggtt
    10621 gttgatcacc ggcgatcgtc caccgcggca gttgaatctg ggattaccgg atctcgcgtc
    10681 gcgactcgac tgggggcaga tctacaaatt gcagccactt tctgatgaag ataagttgca
    10741 ggcgctacag ttacgcgcgc gtttgcgtgg ttttgaactg ccggaagatg ttgggcgttt
    10801 cttgctgaag cggctcgaca gagaaatgcg cacgctattt atgacgttgg atcagttgga
    10861 tcgtgcgtcg attaccgcgc aacgtaagct gaccattccg tttgtgaaag aaattctgaa
    10921 gttgtagata aggtgtttat tatcggatgc ggcgcgaacg ccttatccga actacgcgaa
    10981 gttaatccat agtattcgta ggccggataa gacgtggcaa gcgtcgcatc cggcaatgtg
    11041 cttcaactag tgatggagca catcttaacc cacgatttcc agtacctgct ccggtggacg
    11101 accaatccgc gctttgccat tcgcgaccac aatcgggcgc tccatcagct tcggattatc
    11161 caccattgcc tgaatcagcg cctcttcgct aagcgaactg tcagccaggt tcagttcttt
    11221 ataaagatcc tctttctggc gcatcaattc acgggcgcta ttcatcccca gcattttcag
    11281 caaatcgcgc agcgttgccg catcggcggg tgtctcaagg tagagcacca cttctggctc
    11341 cacgccgttt tctttcagca ggttcagcgt ttcccggctc tttgaacagc gtgggttatg
    11401 gtagattttt acctgtttgg tcattgttct tcctttaatg cgaattacat cttggtataa
    11461 ggcttaaagc gttcctgcag ctggcgcaac tggtcgatgc gcgcatcgta acgcgcttgt
    11521 tgcagactgc ctaatttcac ctgcgaactg gcgctactca acagcgaaat ggcctgatcg
    11581 agtcgtccgg cgagcgcata accttctgct cgcgcagcca gctcctgatc gcggttattt
    11641 agcgcggctt ccgcctgtgc cagcaaatcc cagccgttgc tgtcatcttt attattaaag
    11701 gtgtagcgat tcagaatatt ggccgcttct tgtggttgac cgccttgcag ataagagttc
    11761 gccaggttga gctgcaacac cggattggtg cgtaaatcgc gggcattttt cagacgattg
    11821 atcgcctcat tggctttgtt ttgcccaaga tcgatatcag tagccagatc gagataccat
    11881 gcgttgccag gttctgccgc cagtaacggt tgcagcgttt tacgcgcctc gtcgtattta
    11941 ttggcttcca tcgcctgtaa agcacgacca tattgcgccg ctcgttgctg acgaacgttt
    12001 cctttcgccc attcatccag caaatcactg gtgagctggt tacgtccgga attatacatc
    12061 cccagtgtgc gcgctttcgc cagatagaaa tcttccgacg actgcaccac catcgggcgc
    12121 atctgattag cacggttgcg ggcatctgcc agacgacttt ccggcaacgg gtgagtcaat
    12181 aaaatttccg gcgggcgcga ggagtaacgc gcctgatcga gtaatttttc gaggaaggtt
    12241 ggcatcgcct gcggatcgaa tcccgagcgt tgcagcacct gaataccaat gcggtccgct
    12301 tcctgttcat tttgctgggt gaaactgatc atcccctgac gcgttcccgc cagtgtaccg
    12361 gtcagtgccg ccatccccgc ctgcggactg gccatcgcca gtaaaataga acctaacgcg
    12421 ccgacccagg tcagcggcgc gctgcgctgc tgatcttcca tcgctcgcgc caggtgacgt
    12481 tgggtgacgt gggagatttc gtgcgccata actgaagcca gttgactttc gttatcggaa
    12541 taacggaaca gggcagagtg cagcaccacg ttgccgccaa agaaagcaaa ggcgttaatt
    12601 tcgtcgttgt tgatcagaaa aaaatgaaac ggtgtcttaa ccgaattggc atgcgaaacc
    12661 agacgcatcc ccagcgaatt aatatattgc gttaacagcg ggtcattaat taacggcgcg
    12721 ctgccgcgta gctggcggac ataatagtcg cccatctgca tttcctgacc aatggaaagc
    12781 gtgcttcctg cggaggttcc catatccggc aaggtgtctg cgctgtcggc aaacgccggg
    12841 gctacctgac caatggtcat agcagcaatg agggttgcaa ccaggttttt tttcaactgc
    12901 ctgaacataa cctctatcct gtatttctgg agatagtcat ttgaccgata ctgtgaaata
    12961 tcgttcccac cgcgccgcct ttgagtgtaa cggcgaagat tagcccactc aaatgacttt
    13021 tcagtttcct gaatccgtca taaaaagcga agtctttttt gtgacatttc gatacaattg
    13081 cggatcgcaa tcccgctctt gaggttcagg gaaggtcttt atgctcgaaa tgttgatgca
    13141 atggtatcgc cgccgtttta gcgacccgga agcgattgcc ttgctggtta ttttagttgc
    13201 cggatttggc attatctttt tctttagtgg cctgcttgct ccgttgctgg tggctattgt
    13261 gctggcctat ttgctggaat ggccaaccgt gcgcctgcaa tctattggct gctcccgccg
    13321 ctgggcgacg tcgattgtat tggtggtttt tgtcggtata ttgctactga tggcgttcgt
    13381 ggtactgcct atcgcctggc aacagggcat ctacttaatc cgcgatatgc cggggatgct
    13441 caataagctt tctgactttg ccgccacgtt gccgcgccgc tatccggcgt taatggatgc
    13501 gggcattatt gatgcaatgg ccgaaaatat gcgcagtcgg atgctgacca tgggcgattc
    13561 ggtggtgaaa atttccctcg cctcgctggt cggtttgctg accatagccg tctatctggt
    13621 gctggtgcca ttgatggtct tcttcctgct gaaagacaaa gagcagatgc tgaatgccgt
    13681 tcgccgggtg ctgccgcgca accgtggact ggcaggacag gtgtggaagg agatgaatca
    13741 acaaatcacc aactatatcc gcggcaaagt gctggagatg atcgtggtag ggatcgccac
    13801 ctggctgggg ttcttgctct tcgggctgaa ctattcgctg ctgctggcgg tgctggtcgg
    13861 cttctcggta cttattccgt acattggcgc atttgtggtg accattccgg tggttggcgt
    13921 ggcgctattc cagtttggcg caggcacgga attctggagc tgcttcgcgg tgtatctgat
    13981 tattcaggcg ctggacggca acctgctagt accggtgttg ttctccgaag cggttaacct
    14041 gcatccgctg gtgattattt tatcggtggt gatcttcggt ggtttgtggg gattctgggg
    14101 cgtattcttc gccattccat tggcgacgct gatcaaagcc gtgatccacg cctggcccga
    14161 tgggcaaatc gcgcaagaat aatcttcagc ccgacgaact gtttcgccgg gctgattttt
    14221 caggattcct gacgtaaata aatagcccga taacaaatac tcaccagcac tgcaccgccg
    14281 ataatattac ccagcatcac cgggagcaga ttggcggtaa taaaatggct gaccgtcagt
    14341 gccggaaaat tgtctgcgct actgtgcgcc agttgccaga aggaggtagg ggcgaaatgg
    14401 cgaatggcaa tggcgaacgg aatcacaaac aaattggcga tacagtgctc aaagccactg
    14461 gcgacaaaca gggtgatggg caaaatcatg gcgacgattt tgtcgcataa cgaacgcccg
    14521 caataactca tccacagcgc caggcaaacc atcagattgc acataatgcc gaggctgaca
    14581 gattcagtaa atgtatgatg cattttgccc tcggcgcagt gtaaaaccgc gactccccac
    14641 atcgcgtttt cactcatcac cagcccggaa aaccagatta acaaactgaa acaggcaata
    14701 cctgccagat taccgcaggc caccagaagt gcgttaatca gccaagttcg ccaactaata
    14761 acgccccgac tttttgccat taccgtcatt accgacgagg tgaacaggct ggtgccgcaa
    14821 accgccagca aaataaagcc gagtgtaaag caaaggccgc ccaccagatg ggttaatgcc
    14881 tgtgaagacg gggcatcggc gataacggaa aggtaaaaag taaatccgat cgccataaac
    14941 acgccagcgg acatcgccat taaaaagacc gacactttac tgcgggcaat tttatgggcg
    15001 gcaatccgtt cagcgattgc cgcttttctg gcgctcaact gcaagtcgaa agagagtttg
    15061 tttctcatgc atactcctgc aaaagcagat tacaacacct cgcgaaccga gatccccaga
    15121 cgctgcattc gtgacagcag cgtggtgcgc ttcatcccta atcgtgtcgc cgcgccacgg
    15181 ggcccggcaa caatgccatt ggtttctcgc aatacctgaa caatgcgctg acgttcttct
    15241 tcgtcattct ctggcgtcgt cgggtgcagc aactgcgcca ttgaactgcg aagcgctgaa
    15301 tcttcattta gcgtcggcag taaacggctt tgtcggacat taagatgtaa attcagactg
    15361 ttaccgcgag tcagcagtac cgcccgctca atcacgtttt ccagctcgcg cacgttgccc
    15421 ggccaatccc acgacatcaa ctggcgtagt gcctcagtcg ggatggcgtc aattgcgcga
    15481 ttcatatggc gcgccatttt ttgtgtgaaa tgttttgcta aaagagggat atcttccgga
    15541 cggtcgcgca gtggcggcag ttccagtggg aagacattca ggcgataaaa gagatcgctg
    15601 cgaaactggc gatcttcaac catttgccac aaatcacggt tggtggcggc aatgactcta
    15661 acatttaccg ggatcgttct actcccgccg agacgctcaa tttcccgttc ctgcaatacg
    15721 cgcagcagtt taggctgaag ttctaacggc agatcgccaa tttcatcgag aaacaacgtg
    15781 ccgccatcgg caatttcaaa acgaccacga tgggtattaa tcgcaccagt aaatgcccct
    15841 ttgtcatgac cgaataactc actttccaga agactggcgg ggatggcagc gcagttgatt
    15901 ttgaccagcg gcttgtcgcg tcgcgggcta agttgatgga tcgctctggc aatcacctct
    15961 ttgccggttc cggtttcacc acaaatcaac accgtactgt cgctcttcgc cacaatatct
    16021 acctgctgga gcaggtcttc cattgcctgg ctttgataga taatgtcacc gacgcccaga
    16081 ttcgaaagga gctgctcgct aagctggtgg ttttcttgct gcaaactttt ctgcaaatcg
    16141 gtcatgctac gccaggcatc ggcattgcca acggcaatag cgatgcgatc ggctatgtgt
    16201 tgtagtagct ggcagttttc ctcactaaag agagtggaag aggtatgcgc cagcaacaat
    16261 gcacccggtg tatggttgcc aaaggtaagc ggtataagga gcgcagattc gcagccgtta
    16321 gatgcaagta agtgtaataa cgggtcgcgt tgccagagaa acagatcgtc tgcacggtgc
    16381 gtcaaggtcg gttgtcgggt ttgtagcgtt tgtatcagca atacactgtc gccaggcata
    16441 ttgcgtggca gacacgcaca atgtgaggca gaaagatcgc tgcaccacag gctgaacttc
    16501 tcgttctttc gatgatcgcc cagtaccata ctgactgaag ccagaccgaa aaaatgatgg
    16561 atctcacggg cgacgtcagc gatcagatcg tcgagatcaa gatgtgacag cactgcatta
    16621 gtgatatcca ccagtacccg atgatgatcg cgatctttct ccagcaaacg cagtgccacc
    16681 tgttgttctt catcattgag ccatgattgc agaaccgggg tgattatgcg tgtcagccag
    16741 ttaaacagtg tgcaatcctg gggagtaaaa cgactgccgt gggtgcgaat aaagcgtagc
    16801 tccgccattc cactactgcc gagaaggacg gcgtggcgtg aataatcgac catctcgccc
    16861 tgatactctg ctgttgccgt cacatcgctg ccatcatcat ggtagcggta atagaagttg
    16921 cttttgagaa catgcagttc aataagttgt accgggctga acggcggcgg cagcgggatg
    16981 aaggcgcgta ataaagacgc cttgccgtgt ttctgtgcta accgctccgc gagcattccg
    17041 tttaccgctt caattgttat tccttgtggc ggggcaaaca tcgcctcgtc tgacatagcc
    17101 atcaacagtc cttattaatg agaaatcctt ctcacgcaat ttttctggcc atcagataga
    17161 tggccggttc ctgcacaatt tcatccaaca gatgaatgaa ttgaatgcta cgcgcttgcc
    17221 acacagacgt cgcgtcatct gccagtggcg cgaatgcccg ggtcaacagg gcggtgtggc
    17281 tgacgtcaat aacaatctca ccaaaagtca tcacccccag catcttacgt cgggcttgct
    17341 cctcttcgac cagtgcaagc caatctttgt attccgtcag tgggcagcgg aaggcgacat
    17401 tcagacaatc aatcacgcca acgtgatggc cgatagccag cgagtaatac attacctgag
    17461 agctgtgttc cggcatctcg tcgctactgg cgacaaactt ttttcgcagc gtccagaaaa
    17521 caatttcccc gcactcttca gtcatgtaac ccccggagta cgcgaaagag ttgctgaacg
    17581 atactattga gacgtggatc gtcggcatcg cgcaaccagg tgttaacccg atttcctgta
    17641 ggatcgtcgc ttaaatggcg caggagccga tcgcaaattt ctcgcccctg acgatagccc
    17701 gccagccgcc gcgcttctcg ctcaatggcg atacgctgtg atggcgggat ctgcggccac
    17761 aacggttgca tagtcacccc ggtgggatcg cgataatcca ccgcgtgaat cttctgttgc
    17821 agcaaaccga gcgccacggc gaaaccgtga atggtggcgg ccggtgttgg cgggcagccg
    17881 gggatccaaa catcaatggg gacaatggtg tcgctgccgc cccagacgct gtagagatcg
    17941 tggaaaatac cgccgccgac accgcacgcg ccgtaggaaa cacagatttt atgatcgggg
    18001 gcagactcat acgcccgaag tgcaggcata cgcatcgccc gggtgactgc gccagtaaat
    18061 aacaaaatat cggcgtgacg cggtgatgaa acaaccttta tgccaaaacg ttctgcgtcg
    18121 aatacgggtg tgatggcggc aaaaatttcg atttcacagg cgttgcagcc gccgcaatcg
    18181 acgcgataaa cgtaggctga gcgacggata tcctgtagca aatgcagctt catcttttgc
    18241 gtttgctcgt ccagcgtgat gggctggctg acatgttgtg taagcactgg actcatagct
    18301 gctccttagc caccagcggt acatctgtat cgtcgttgat cagcgtcgcg cgttgtttgc
    18361 actccgggca gacgctcgct tgcgcccgca acatttcgcg gttttgtggc gcattttgtt
    18421 gctgtgccaa caattcagca gccagtgcga cggttttttg cggggcaaac gggcgttcgc
    18481 aacggctgca acgttgtaga tggaacgtcg cgcgggtata gagatcggct ttattggtga
    18541 cggtcagttc aaagttattg gtaagctgga tggctctggt cgggcacact tcttcacaac
    18601 gtccgcagta aatacaacgc cccagataga gctgccaggt gcgcgaattt tgctggtcgt
    18661 cggtctggat agtcagcgca tttgccggac aagcacaggc gcaggcaccg caggcaatac
    18721 attggctggg catcaggtcc ggtttcccgc gaaagcctgg gctgacctcc agtggcgcga
    18781 agggatattt caccgtcgcg gttccggcgc gcataatagt tttcagtaac ttcagcatct
    18841 tctgtcctta cttcagcggc gaacggttac gatcaatgcc gtagcgttcg atctctttat
    18901 acggcacggt ttttgactgg cgcttgcgca catctaccag cgtcacacgg tcggtacagg
    18961 agtagcaggg atcaaggcta ccgataatca gcggtgcgtc agaaacggta ttgccgcgta
    19021 gcatgtaacg cagcaccggc cagttggcgt aggtggcggc acgacagcgc cagcggaaca
    19081 atttttgatt atcaccgagc atgctccagt gcacgtcttc accgcgtggc gcttcaacaa
    19141 agccgagcgc gaatgcgtga ggtttatagc taaagccttc ggtcagcagc ggggtatccg
    19201 gcatgttgtc gagggcaaat tccagcattg ccagcgaatc aaacgtctct ttgacacgga
    19261 ccatcacgcg ggagaagaca tcgccgccgg taaaggtaaa cagtgttttt ggaatattgc
    19321 cgtagtcggc gtaggggtga tcaaagcgca aatcacgggc aaaaccactg ccgcggatca
    19381 gcggccctac agggctataa tcacgggcga tttgtcggtc gagaatgccg ataccctgag
    19441 tgcgttgttc catattcggc gtggcaagca gcatttctac cagctccgac acgtcggcgc
    19501 gcatctcgcg caccagtttc agcgtttgca gacgttgctc tttgagaata tcgcggcgaa
    19561 caccaccaat cagattcaga ccgtaggttt tacgcgaccc ggtcagcaat tccgccattg
    19621 tcatcgactt ttcccgcacg cggaaaaatt gcataaagcc ggtatcaaaa ccaacaaaat
    19681 ggcaggagag gccaaggttg agcaaatgac tgtgtagccg ttcgacttcc agcagaatcg
    19741 agcgaatagt atgcgctcgt tgcggcacct caatccccag tgcattttca accgagttgg
    19801 tataggccac actgtgggca aaaccgcaaa ttccacacac gcggtccgat aagaaggtca
    19861 cttcgttgta gcccatccgt gtttctgcca gtttctccat gccgcgatgg acataaaaca
    19921 ggcggtaatc agcatcgaca atttgctcgc catccacaaa caagcggaag tgacccggtt
    19981 catcggaggt gatatgcagc gggccgacag ggatcacccg cgcatcgctg ttgccctcat
    20041 tgataaacgg atacgtttcg gaatcagtcg tcggttcagg gcgcagtcga taatccatcg
    20101 catctttgcg tagcggatgc atatcttccg gccagtcatc gggcaacacc aggcgacgct
    20161 gatccggcag gccaaccgga atcagcccgt acatatcgcg tatttctcgc tcgccccaga
    20221 ccgcggcagg gacgcgcggt gtgactgacg gaaactcccg actgtcggca tcgaccagtg
    20281 ccttcaccac gatccagcat ttttcggccc cttccataga aagggcataa taaacggcgt
    20341 aatgaccgtt aagtgtccgc tcgtcgttgc caaacaggac cggaagccag ccatcatgtt
    20401 gataataaag ataatgtaca acgtcaggca gcagattgat tttcaccgta atggtgacct
    20461 gttcaggcgt ttgtcgctct tcatccagca ccgcgccggg gaactgcgtt ttcagggcgg
    20521 cgagaatcgc ttcgccacga tttgacgatg aattaacgtt cactgtgttt ctccgaaaca
    20581 gatgcggtgc ctgaaggcaa aaaatcatgc caggtgctac gttgtgcagg caggtcgtgc
    20641 gtccctgaga ggactatagt ggaagcgccc gccaggatcc tgatgacagg ttgtggaata
    20701 tgcgttccca tcgccagcat catgaccagc agaatcacca ttggcgaggt ggtcaaccag
    20761 ccgagatcac cccggttaac ggcctgcggc ggtttcgcca ttaacacccg cgcagccatc
    20821 cgtaccaggc ccgccagcac cagcgttaac agcaataaca gcaggacgat aatcagcaga
    20881 tgattacgtg ccagtccggc ggtaacggtc ataaattcgc taagaaaaat gttgaagggc
    20941 ggcatccctg ccagcgccag cgcaccgccg ccaaacagca cggcggtaaa tggcatgatt
    21001 ttgagcatcc cacagacgac gttgagatcg cgcgtgccgt acttgagcag tacattgccg
    21061 gaaccgcaga acagcagcgt ttttgccaga ctgtggttta aggtgtgcag cagcgcggca
    21121 aaaattccca gcggcccgcc aatgcctagc gccaccgcga ccagtcccat gttctccacg
    21181 ctggagtacg ccagcagacg cttaatgtcc cgctgtacca gaatgaaaaa tgccgccacg
    21241 gcaaccgaca acatgccgaa gatgagcaac aaccggttgg ggaaatcgct gccgatggcc
    21301 tggcaaataa tgatgtaata gcgaatcagc accaacagcg cgcagttcag caatacggcg
    21361 gagagtaggg cgctgaccgg actcggcgct tcactgtgag catccggcag ccaggcgtgc
    21421 atgggaaata gcccggtttt ggtaccaaag ccaattagca caaacacaaa ggccaacagc
    21481 attaatgttg ggtcaagcaa ggacgattgc ttaagaactt cgctccagaa tatcgccatt
    21541 tctgcctgcg gcataacgct ggcggcgttg gcgtatacca gcacggtacc gaacagacca
    21601 aaagcgacac caacagtaca aataatgatg tacttccatg ctgcttccag cgatgaacgc
    21661 tgaccgtaaa tgcctaccag aaacgccgag cttaaggtgg tggcttcgat cgccgcccac
    21721 atcacaatca ggttattgct ggtaacaacc agcagcatgg tgaacaaaaa cagatggaag
    21781 aaaccgtagt aatcgcacag cgtaacgggt gaaagctcgc cgtgctccac ttcatgacgc
    21841 atgtagccaa tcgagtaaac accggtaaga aagccaatca caccaagaat ggctaggaac
    21901 aaaccgccca gaccatcaat atgaagccac agtcccgcag cgaatatttc tcccgcatca
    21961 gcggcggttt ggaccaccca gagtgccaga atcagcagca gtgtgatccc taagctatgt
    22021 aatactgtca ccgcgcgagt cgcagagtgc ccccgtttcc ggcaggcaaa acagagcagc
    22081 gaaaaaagca gcggcgtgag caggagtaaa gcgaacatca cagaataact catctcatta
    22141 tcccttcagc gcggtcaagt tgttcacgtc cagcgtgccg tgggtacgcc atatttttct
    22201 tgccagtaac accatcacaa tgacggcgaa gatggcgtcg gtagcgatac caatttccac
    22261 cagttccggt gcacgccagg caagaagcgc tagcaccagg tgggagccgt tttccatcag
    22321 gcagtaacca aaaatttgcc gcaggatatt gcgctggctg acaatgcaca gcaggccaag
    22381 cagaaaatga cctaacgcca ccgccagcgc aggtttcagc ccggtagcca tcggtagctt
    22441 cacgggctga acgacaaatg cgcaaagcag gacaattaac gccgcgagca gtgtcatcat
    22501 tgccggaccg aataacgctt tttccgggat gttctgggga ttatttcgtg cagcgtaagt
    22561 catgattaac ggtaccagca gcactttggt gataaaggcg ctggcggacc agatcagcag
    22621 ttgctctgcg gcgaacaggc acgagagagt ggcgaaaata gacaccagca ccagcgactg
    22681 gcaggcgtaa aatccgcatg acaggcgata gcttttgacg ctaatcacaa acagcgatgt
    22741 cagcatcatc agtcccgcca gattatttac gatcatagaa ccggtcattc atcatctcct
    22801 tactgcaacc aggtcgcggc gattacgctg gaaatcagcg acatcacaat cagtacaatc
    22861 agcaccaggc gcattgaccc tggcagcggt gcggcatcgg cgacggcctc gctcggtttg
    22921 ccggggacga cgcgaccaaa ccagcgaata aaccaggcga aactggcgac cgattcaatc
    22981 atcagcagaa tcatggcggg cagcaggatc cagtactcca ctgacaacgc aaaaccagca
    23041 gcaaacagcg ggaatttact gaagaagccg ttgaaaggcg gtacgccggt aatcgccagc
    23101 gctgccacgc agaaacccac gcctggcaac ggcagggtgt gcaatacgcc acgcagaagc
    23161 ggcaacaagc gcgtgccgca gctgtaactc agcgcacctg ttacaaggaa aaacaggctt
    23221 ttagcgaacg cgtggttgac gatgtaggcg atgctaccct ccagcgccag ccgcgagccg
    23281 aagatggaga gcgacaagcc gaagaacatc cagccaagtt gagtgatggt cgaccaggcc
    23341 agcaatcgct tcatatcctg ctgtggcaaa tacatcagaa atccataaag aatggtgacc
    23401 agggccatga ccatgccaac gccgccaatc acatgcggga tattgccgcc gtcgataatg
    23461 gcgcgggcaa aaatgtaaac gcccactttc accatcgatg cggcgtggag ataggcgctg
    23521 atcggtgttg gcgcttccat tgcgtccggt agccacgctt gcatcggtag ctgggccgat
    23581 ttcccccacg cggcaaacag gatgccgcca taaaccagat aacgtgcgtc gccgtgtaac
    23641 tcgctcatcg cgctaagcgc aaacgttccg gtctgcaaaa acagcgtggc ggcggcaaga
    23701 tacaacccca acgaaccgat atgggtgata agtaacgctt ttagtgccga acgctgcgct
    23761 ttatcgctct ggtaataact gatcaacgcc caggagcagc cgcccgtaat ttcaaaaaac
    23821 aacaactgac cgagcagcgt cgaggagagt accagtcccg ccatcgcgcc gataaacacc
    23881 agcaaaaatg cgtaataacg attcgtaccg ttatgcgggt gttcgcgatt tttatccgtc
    23941 agataacccg tggagtagat cgtgaccagc aaaccgagaa acaccaccac aaacagaatc
    24001 agcgtactca cgcgatcaat gaccaggcca aacaatgcca catcccccac gttgaccaac
    24061 gtcaacgtga cggcaacttt atcggcctga taaaacgcgg agatcagcga caacatgcac
    24121 agcgtggtca gcgcggcgaa caaaaccccc cattcggcgg cccgacgttg tggcgaaaac
    24181 gaaacgacca gtgcgccgat aaaaggcagc attaacgtcg tcagagcaag attttccata
    24241 ttccgtcagt gctccttaca gaccggttaa ccagaagacc catgcaagcg cagcaaggct
    24301 gaagccaagc caggtcacat ggtgaatgag taaaaaacgc ccgcgtgcca gcgtgttttc
    24361 tgcgatagag gccagtacaa aaatcagcaa aaccttgagc agcgtaacga caagtgaagt
    24421 cagcaggcag gcgagagaaa gttcttgcgc gcgcccaaag ggcagaaaca gggccacaaa
    24481 cagtgctgcc atcacgacct gtttcagccc cagcccccat ttcgccagcg ccagcccggc
    24541 accggaatat tctgtcagcg ggccttcctg taattcctgt tctgcttcag caacatcaaa
    24601 gggaattttt cccatctcaa tgaagcaggc aaaaccacag gccagtaacg ccagtacggt
    24661 ggttagcggc gagttccagc ccgttgccag cgtattgctg atcatctcga tatgcgtgga
    24721 acctgctatc agcgccaata ccagcagtga gagaatgagc atgggttcga ccagaatgcc
    24781 gagcgtcaac tcgcgactgg caccgactcc cgcaaacggg cttccggtat ccagcccgga
    24841 aagagcaaag aaaaaacgaa acagggcaag aagatagata agggtgatca gatcgccgcc
    24901 gcccgcaaaa ggggaaacgg taataaatag tggtagggcc atcgccagca ccagcatgct
    24961 gctgattaat acccacggca tcaggcggaa catcagacct gaagatgtcg gcgcaacttc
    25021 ctggcgttta aacagtttgt ggatatcgcg ataatcctgc cagatccccg gtccgcggcg
    25081 ggagtgcatt cgcgcgcgta tctgccggga aatacccgta aaaagcgggg ttagcagcag
    25141 aatcacggcc tgtgctaacg caaaaatgac cagatatccg tcgcaaagag tttgtctcat
    25201 ggtgatttcc ttagacggca atagcgatta gcagcacaac cagtgcggcg accacataca
    25261 ggcaatagag acgaaagtcg ccgctttgca gatgctgtat ttctttggcc agccgttggg
    25321 tggcgctcac gatggggcgg atcacccgct catcccagaa gggttctgtg ctctgagccc
    25381 tggcggtgac gtgcgcaaga cctttattta gcctcagcgt agggtcgagc tgttgtcgta
    25441 gacgaaataa cgcagagaag accacacgca gcggctgcat cacgccattg cctgacgggg
    25501 ccatcgcatt ttcccagccg tagccgcatg cccaggcatc tcctgtgcga cgaaacgctg
    25561 cgcggcgcga acgacaccac agccagtaaa ggccaggcag caaaggcatc gccagtagta
    25621 acagcaacag taatgagggg gtgacctgtg tatgaaacgt gccgggtaca agtgctattc
    25681 cgctggcgac agtgacggga ggggtattgg taaacgcatg ggcaatatgc attatcttcg
    25741 gtgccagcca actggcacta agcgcaatta atacgcagag tgccgcgagc agtagcatgg
    25801 cgacgatcat cgtatttggt acttcctgtg cctcttcagc gtgtgtactg cgcggcgcac
    25861 cacagaaagt aataccgtac attttgacga agcacattac tgccagccca ccagtgactg
    25921 ccagcattac catagcaata ggacccgcaa gttgtagcgc tacggcttcc acacgactta
    25981 gtgagaacag cgactgccag gtgtaccatt cgctgataaa accattcagc ggaggaatgg
    26041 ctgatatcgc gaggcaacca atcaggcatg ctgcggctgt ccacggcatc cgtttcgcca
    26101 gtgcccccat tttttccatg tcgtgggtat gcaaacgcga aataatcgcg cccgcgccga
    26161 gaaatagcag ccctttgaac agcgcatggt tgagcagatg aaacagtgcg ccgagcagtc
    26221 caaccacggt gagcagcggg tcgtgcagtg acagaccgac cattgccaca ccgactgcca
    26281 gcaaaataat gccgacgttt tcgacggtac tccaggccag cagccgtttg atatcctgtt
    26341 ccgccagcgc atacagcacg cccaggagcg cggagattgc gccgatcgcc atcaccagaa
    26401 tgccccacca cagcggcaaa cccgtttgcg ccagcagatc catcgccact ttcaggatgc
    26461 cgaaaatacc gattttgacc attacgccag acatcaacgc cgaagcgtgc gatggtgcgg
    26521 cagggtgagc gcgcggcaac cagctgtgca acggcatcat cccggctttc gcgccaaaac
    26581 cgaaaaaggc cagcaggaac accgccgacg ccagccccgg agaaagtgaa agcgtgcgga
    26641 aactggcaaa atcgaggctg ccgctttcgc gccccatcag caagaaggcg atcattatca
    26701 gcaccgatcc ggcgtgggcg ataaaaaagt agagcatgcc agcgttgatc gacgttttat
    26761 cctgcctggc aatgaccaga aaccaggaag acagtgacat catttcaaac agcacgatga
    26821 accaaaaagc gttgtccatc accagcaggg caaccatcga tgcgatgaaa agattcataa
    26881 agaagcccat cgccgccgcg cctttgccct cgtattcgcg catataagtc aatgaataga
    26941 gcgaacaaac taccaccagc aaggagataa ccagcaccat aaatgccgag aggctatcca
    27001 ttcgcagggt taaatcggca aagtcaaaag gggtggcaaa acgggcgact aaagttacgc
    27061 ctgcatgtaa ttgcgtaatt ccgctgataa tgccaatcac gccgcccacc agcgatgtga
    27121 tgccggaaag cttaatagcc agtctgtcca gaccgagtaa aaacagcgaa gccagactgg
    27181 caaacagata gagaatcagc gaccaggtta ataattgcag ggcatccatt agcgttgctc
    27241 ctgagtgagg gaaaggggat cctccccgcc attggcaaac cagcttgcgg caaggcgctg
    27301 tttttctttc atctgacgtt gcaggctatc gtcggtgatc agccgtaacg cctgattcgg
    27361 gcaagcgcga acgcaggccg gaccttgtgg caagaaatca cacaggtcgc atttcaccgc
    27421 gacggtctgg acacctggtt cccagcgcag caaagcatgt tgggttggcg cgttttcttc
    27481 gccgtctttg agtgagcctt cagcctgaaa aacatattgc gcaggggcat tcaccggacg
    27541 gcttcctgaa gcgctgattg cgccaaatgg gcagaccacg gcgcaaagct tgcagccaat
    27601 acagaggctt tcgttgagtt ggatcgcatc atccctctgg gagatggcat tgaccgggca
    27661 gacctgcagg caaggggctt cctcacagtg atgacacacg acaggggcag tgagtgttga
    27721 cgtcttcgcc agggccaggc gcgggtgttg ctgtaaacct tgcgttttat gcacgtccga
    27781 acaggcagcg agacaggtat tacatcctgt acaccacagt ggttcggcca ccacaaagcg
    27841 gttcatgtcg gctccttgaa aatgatatga aacattgcat ttcatattgc ataaaggatg
    27901 ccagtctgat taatttatta tattcaataa gttatgaatt aactggattc tgaacggtac
    27961 aaaaatgatg gtcattaacg ttcagcaaaa aatggattcg acacccgtgt cgcaggggaa
    28021 agcgagaatg tgatcttgat cacgataagt gaggtgtgct gaccacaagc gcagccagca
    28081 cggaatggag caaagtaatc aggcgtgttc tttcagccag ttcagcacaa cgtcgtggtg
    28141 attgctggtt ttgaaatcgt caaagacatg ttcgattttg ccatcagcgt caatcaggaa
    28201 gctgatgcga tgaataccat cgtaggtttt gcccatgaag gacttttcac cccagacgcc
    28261 gaattgttcg cacacctggt ggtcctcatc agacaggagc gtaaagttaa gcagctcttt
    28321 ttccgcaaaa cgggagagtt tttcgggttt atcggtgctg atacccagca catcaacgcc
    28381 cgcttttttc aactcatcca tgttatcgcg taagccgcag gcctgtacgg tacagccggg
    28441 ggtcatggct ttcgggtaga aataaaccag aacacgctgt ccctggaagt cggtcaaatt
    28501 aacttgttct ccgtcttgat ccggcaagct aaatttcggt gcgatatcac cggctttcag
    28561 tggattcatt acttaactcc atcctgttca tcatgttggg aataattgac gacgttaata
    28621 ctgccttgtg cattgagttc tgtacatagg gctttgaacg cttgctcaat atttgctgcg
    28681 tccgcagatg cggggctgtg ggcagtaatc tgaatatgca actgcgcagc ccgttcattt
    28741 tcagcaggtt gtgtgcgcga caccagctcc gcaatgttca tatgatgcgt gtcgaaaagt
    28801 gctgtgaagc gttcaattaa atgcggggag tctgccacat cgacctgaac ccagacagat
    28861 gctggcattg gcggacgcgg acgcgccgtc gtgcgcttca tcacgattaa aagatccagt
    28921 tcggcacctt tcaacggtaa cgttgattca atcagagtaa tggcattcca tgaaccggaa
    28981 agcagcatga taaacgtgaa ctcttctccc agcatcgcca ggcgactgtc ttcaatatta
    29041 cagccgcaac tactgacatg acgggtgatg gtgttcacaa ttccagggcg atcggcaccc
    29101 aacgcagtga tcaccagata atgttgcgat gacagtgtca aactggttat tcctttaagg
    29161 ggtgagttgt tcttaaggaa agcataaaaa aaacatgcat acaacaatca gaacggttct
    29221 gtctgcttgc ttttaatgcc ataccaaacg taccattgag atacttgttt gcacagagga
    29281 tggcccatgt tcacgggaag tattgtcgcg attgttactc cgatggatga aaaaggtaat
    29341 gtctgtcggg ctagcttgaa aaaactgatt gattatcatg tcgccagcgg tacttcggcg
    29401 atcgtttctg ttggcaccac tggcgagtcc gctaccttaa atcatgacga acatgctgat
    29461 gtggtgatga tgacgctgga gctggctgac gggcgcattc cggtgattgc cgggaccggt
    29521 gctaacgcta ctgcggaagc cattagcctg acgcagcgct tcaatgacag tggtatcgtc
    29581 ggctgcctga cggtaacccc ttactacaat cgtccgtcgc aagaaggttt gtatcagcat
    29641 ttcaaagcca tcgctgagca tactgacctg ccgcaaattc tgtataatgt gccgtcccgt
    29701 actggctgcg atctgctccc ggaaacggtg ggccgtctgg cgaaagtaaa aaatattatc
    29761 ggaatcaaag aggcaacagg gaacttaacg cgtgtaaacc agatcaaaga gctggtttca
    29821 gatgattttg ttctgctgag cggcgatgat gcgagcgcgc tggacttcat gcaattaggc
    29881 ggtcatgggg ttatttccgt tacggctaac gtcgcagcgc gtgatatggc ccagatgtgc
    29941 aaactggcag cagaagggca ttttgccgag gcacgcgtta ttaatcagcg tctgatgcca
    30001 ttacacaaca aactatttgt cgaacccaat ccaatcccgg tgaaatgggc atgtaaggaa
    30061 ctgggtcttg tggcgaccga tacgctgcgc ctgccaatga caccaatcac cgacagtggt
    30121 cgtgagacgg tcagagcggc gcttaagcat gccggtttgc tgtaaagttt agggagattt
    30181 gatggcttac tctgttcaaa agtcgcgcct ggcaaaggtt gcgggtgttt cgcttgtttt
    30241 attactcgct gcctgtagtt ctgactcacg ctataagcgt caggtcagtg gtgatgaagc
    30301 ctacctggaa gcggcaccgc ttgcggagct tcatgccccg gctggaatga ttttgccggt
    30361 gacctccggt gattatgcaa tcccggtgac caacggtagt ggtgctgtcg gtaaggcgct
    30421 ggacattcgt ccaccagccc agccgctggc actggtttct ggcgcgcgta cccagttcac
    30481 gggcgatacc gcttcattgc tggtggaaaa tggtcgtggc aatactctgt ggccgcaggt
    30541 ggttagcgtg ctgcaggcga aaaactacac catcacccaa cgtgatgatg ctggtcagac
    30601 actgaccacc gattgggtac aatggaaccg tctggacgaa gacgagcagt atcgtggtcg
    30661 ttatcaaatc tctgttaagc cgcagggtta tcagcaggcg gttacggtta aactgctgaa
    30721 cctggaacag gcgggcaaac cggttgcaga cgcggcttcc atgcagcgtt acagcacgga
    30781 gatgatgaac gttatttccg ctggtctgga taaatctgcc actgacgccg cgaacgctgc
    30841 gcaaaatcgt gcctccacca ctatggacgt acaaagtgca gctgatgaca ccggtttacc
    30901 aatgctggtc gtacgcgggc cgttcaatgt ggtttggcaa cgtctgccag cggcgctgga
    30961 aaaagtgggc atgaaagtga ccgacagcac ccgttcgcag ggcaacatgg ccgtaactta
    31021 taagccgctg tctgacagcg actggcagga actgggcgcg agcgatccag gcctggcatc
    31081 cggtgactat aaactgcagg ttggcgattt agataaccgc agcagcctac agttcatcga
    31141 tccgaaaggt catactctga ctcagagtca gaacgacgcg ctggtagctg tcttccaggc
    31201 tgcgtttagc aagtaaaaat acagggctgg aatcatccgg cccttttttc tgatatgata
    31261 cgcaaacgtg tgcgtcggca ggaaaacgcg attttagcgg taattcgcac gaaatttgtt
    31321 tgtcggacgt agttcggata aggcgttcac gccgcatccg acaaaacatc cggcacacca
    31381 gacagcaaaa gattttaaaa cgttaattca cacccaggag tgataaagat gcaaaagcaa
    31441 gctgagttgt atcgtggtaa agcgaaaacc gtatacagca cggaaaaccc ggacctgttg
    31501 gtgctcgaat tccgcaatga tacgtcagca ggggatggcg cgcgcattga gcagtttgat
    31561 cgcaaaggta tggtgaacaa caagttcaac tacttcatta tgagcaaact ggctgaagcg
    31621 ggtatcccga ctcaaatgga gcgtctgctc tccgataccg aatgtctggt gaaaaagctg
    31681 gatatggtac cggttgagtg tgtcgtgcgt aaccgtgctg cgggctctct ggtgaaacgt
    31741 cttggaatcg aagaaggtat tgagctgaac ccgccgctgt tcgatctgtt cctgaaaaac
    31801 gacgccatgc acgatccgat ggtcaacgaa tcttactgcg aaacctttgg ctgggtgagc
    31861 aaagagaacc tggcgcgtat gaaagagctg acctacaaag cgaacgacgt gctgaaaaaa
    31921 ctgttcgatg atgctggtct gattctggtc gacttcaagc tggaatttgg tctgtacaaa
    31981 ggcgaagtgg tactgggtga tgagttctcc ccggacggta gccgcctgtg ggacaaagaa
    32041 acgctggaga aaatggacaa agaccgtttc cgccagagcc tcggtggcct gatcgaggcc
    32101 tatgaagccg tcgcccgccg cctgggtgta cagctggact gatttttctg ttcatcatct
    32161 tgccgtgctg ctggcacggc aagacaacca ttctcgtaag atgtgcattg aacgctcatt
    32221 cctccgccat ttcatcccgc tttcctgtgg taatcttcct ctgaaccacc tacgatcatt
    32281 tctataatga atatattgtt gaggtatcta tgcgttggca agggcgacgt gaaagtgaca
    32341 atgttgaaga caggcgcaat agctccggcg gtccgtcaat gggcggtccc ggtttccgtc
    32401 tgccgagcgg taaaggcggg ctgattttac tgatagtcgt gctggttgca ggctactatg
    32461 gcgttgattt aaccgggttg atgaccgggc agccggtttc tcaacagcaa tcaacgcggt
    32521 caattagccc aaatgaagac gaagccgcaa aattcacctc ggtgattctg gcaaccacgg
    32581 aagacacctg gggacaacag ttcgagaaga tgggtaagac ctatcagcaa ccgaagctgg
    32641 ttatgtaccg tggaatgacg cgtaccggat gcggggcggg ccagtccata atggggccgt
    32701 tctattgccc ggcggatggc acggtttata tcgatctctc cttctatgat gacatgaaag
    32761 acaaacttgg cgcggatggc gattttgccc aggggtacgt tatcgcccat gaagtcggtc
    32821 atcatgtgca gaaactatta ggcatcgagc cgaaagttcg tcaactgcaa caaaacgcga
    32881 cgcaggcgga agtgaaccgc ttatctgtgc gtatggaact ccaggccgac tgttttgccg
    32941 gtgtctgggg gcatagtatg cagcagcaag gcgttctgga aaccggcgat ctggaagagg
    33001 cgctgaacgc ggcgcaggcc atcggcgatg accgtttaca acagcaaagt caggggcgag
    33061 tagtaccaga cagtttcact catggcactt ctcagcaacg ctacagctgg tttaaacgtg
    33121 gtttcgacag cggcgatccg gcacaatgca atacttttgg taaaagcatt taacttccgg
    33181 ggcagggatg gctgaactga ctgcgcttca cacattaaca gcgcaaatga aacgtgaagg
    33241 gaaccgccgc ttgctggtgt tgagcgggga agagggttgg tgttttgatc atgcgcttaa
    33301 gttacgtgat gccttacctg gcgactggct gtggatttcg ccgcagccag atgctgaaaa
    33361 ccactgttct ccctcggcac tacaaacttt acttgggcgc gagttccggc atgcggtatt
    33421 cgacgcccgc cacggctttg atgccgctgc ctttgccgca cttagcggaa cgttgaaagc
    33481 gggaagctgg ctggttttgt tactccctgt atgggaagag tgggaaaacc aacctgatgc
    33541 cgactcgctg cgctggagtg attgccctga ccctattgcg acgccgcatt ttgtccagca
    33601 tttcaaacgc gtacttacgg cggataacga cgctattctc tggcggcaaa accagccgtt
    33661 ctcgttggcg cattttactc cccgtactga ctggcacccc gcgaccggcg caccacaacc
    33721 agaacaacag caactcttac agcagctact gaccatgccg tcgggcgtgg cagcggtaac
    33781 ggctgcgcgt gggcgcggta aatcggcgct ggcagggcaa ctcatttctc gtattgcggg
    33841 tagtgcgatt gtcactgcgc ccgcaaaagc ggcaacggat gtactggcac aatttgcggg
    33901 cgagaagttt cgctttattg cgccggatgc cttgttagcc agcgatgagc aagccgactg
    33961 gctggtggtc gacgaagccg cagccatacc tgcgccgttg ttgcatcaac tggtatcgcg
    34021 ttttcctcga acgttgttaa ccactacggt gcagggctac gaaggcaccg gacgtggttt
    34081 tttgctgaaa ttttgcgctc gctttccgca tttacaccgt tttgaactac aacagccgat
    34141 ccgctgggca cagggatgcc cgctggaaaa aatggttagt aatgcactgg tttttgacga
    34201 tgaaaacttc acccatacac cacaaggcaa tattgtcatt tccgcatttg aacagacgtt
    34261 atggcgaatc gagccagaaa cgccgttaaa ggtttatcag ttattgtctg gtgcgcacta
    34321 ccggacttcg ccgctggatt tacgccggat gatggatgca ccagggcaac attttttaca
    34381 ggcggctggc gaaaacgaga ttgccggagc gctgtggctg gtggatgagg gtggattatc
    34441 tcaagaactc agtcaggcgg tatgggcagg ttttcgtcgc ccgcggggta atctggtggc
    34501 ccagtcgctg gcggcgcacg gcagcaatcc actggcggcg acattgcgtg gacggcgggt
    34561 cagccggatt gcagtccatc cggcgcgtca gcgcgaaggc gttgggcaac agctcattgc
    34621 cagcgctttg caatataggc ctggcctcga ctatctttcg gtgagttttg gttacaccgg
    34681 ggagttatgg cgattctggc aacgctgcgg ttttgtgctg gtgcgaatgg gtaatcatcg
    34741 tgaagccagc agcggttgct atacggcgat ggcgctgtta ccgatgagtg atgcgggtaa
    34801 acagctggct gaacgtgagc attaccgttt acgtcgcgat gcgcaagctc tcgcgcagtg
    34861 gaatggcgaa atgcttcctg ttgatccact aaacgatatc gtgctttctg acgacgactg
    34921 gcttgaactg gccggttttg ctttcgctca tcgtccgcta ttaacgtcgt taggttgctt
    34981 attgcgtctg ctacaaacca gtgaactggc attaccggcg ctgcgtgggc gtttacagaa
    35041 aaacgccagt gatgcgcagt tatgtaccac acttaaactt tcaggccgca agatgttact
    35101 ggtccgtcag cgggaagagg ccgcacaggc gctgttcgca cttaatgagg ttcgcactga
    35161 gcgtctgcgc gatcgcataa cgcaatggca attttttcac tgactccttc aattttctcc
    35221 catggtcatt gtgagtcagc ggcgtaaaat taatgacatc agttaaggag ttcgccatga
    35281 aacatgacca ttttgttgtt caaagcccgg ataaacctgc gcaacagtta ttgctgcttt
    35341 ttcacggcgt cggtgataac ccagtagcga tgggggagat tggcagttgg tttgcaccgt
    35401 tgttcccgga tgcgctggtg gtgagtgtcg gaggcgcgga gccgagcggt aatccggcgg
    35461 ggcgtcagtg gttttcggtg cagggtatta cggaagataa tcgccaggcg cgtgtggatg
    35521 ccatcatgcc gacgtttatt gagacggtac gctactggca gaaacagagc ggagtagggg
    35581 ctaacgccac ggcgctaatt ggtttctcac aaggcgcgat tatggcgctg gagagcatta
    35641 aagccgaacc gggccttgct tcgcgcgtca ttgcctttaa cggacgctat gcgagcctgc
    35701 cggaaaccgc gtctacagcc acgacaattc acttgattca tggtggtgaa gatccggtta
    35761 tcgatctggc gcatgcggtt gcggcgcagg aagcattaat cagtgccggt ggtgacgtga
    35821 cgctggatat cgtggaggat ttaggccatg cgatcgacaa tcgcagtatg caatttgccc
    35881 tggatcactt gcgttatacc attccgaaac attatttcga cgaagcgcta agtggcggta
    35941 agcctggcga tgatgatgtg attgaaatga tgtaaaagac agattacctg gcctgataag
    36001 acgcgtgagc gtcgcatcaa gcatcgttca ccattgccgg atgcggcgtt aacgccttat
    36061 ccggcctacg gttgagctta tccggccact cagcttactt cttcggttga tcctttttcg
    36121 gccagtcgtc gtcatcgtcc cacttatcgt tgaaatcacg atgcggggga agctccggct
    36181 tgttggcgag aaatttctta tggtcgacgc gcttgagatc tttaatcacg ttcagcagga
    36241 cgcctaccaa aaagacaatc accagaatcc accaatattt agccagccag tccatgctta
    36301 tttcctcttg cagaaccact catcacgcga cgagctgttc catgatacgt tgatacatac
    36361 gggcaagtag ctgcaggtcg gcagcgttca cacattcatt aattttatga atagtggcat
    36421 tgaccggccc gagttccacc acctgcgccc ccatgcgagc aataaagcgc ccgtcggacg
    36481 ttccgcctgt ggtcagtagc tgcggtttaa tttcattata gtgctcaacc gcgttaacga
    36541 ccgcatccac cagtttaccg cgcgcggtca aaaatggctg cccggaaagc caccagtcca
    36601 ccgtataacg cagttgatgt ttttcaagca gggcaagtac ctgcgctttg atcatctcat
    36661 cagtcagttc ggtactgaag cggaagttaa actgcacaaa cagttcaccc ggaataacgt
    36721 tgttactgcc tgttcccgcc tgaatattgg caatctgcat actggtcgcc gggaagaatt
    36781 cattgccctg atcccactca atagccacta attcattaag gaaaggtgct gcgcgatgta
    36841 ccggattgtc agccagatgt gggtaggcaa catgcccctg aacgccatga atggttaggt
    36901 tgcaggttaa cgatccgcga cgaccatttt tcaccacatc acctaccact tcgatactcg
    36961 acggttcgcc gaccaggcag taatcgagac gctcattacg tgccattaac gcttcgacga
    37021 cttttaccgt accattgtgt gcactggctt cttcatcaga ggtgatcaga aatgccagtc
    37081 gccccgtatg gttgggatgt tgtgcgacaa aacgttctgc agctaccacc atcgccgcca
    37141 gcgagccttt catatctgcc gcaccgcgcc cgaataacat gccgtcacga atggtgggtt
    37201 caaacggcgg attgatccaa cgatcggcgt cgccaggcgg caccacgtcg gtatgcccgg
    37261 caaaggctaa cgtttcacct tgcccacgcc atgcccaaaa attctgcgta tcggcaaagt
    37321 ccatgcgttc aacggtaaaa ccgatcgcct gcaaacgttc aatcaacaaa gcctggcatc
    37381 ctgcgtcatc aggactcagg gaagggcggc gaataagctg ttgtgtcagc tcaataaccg
    37441 ggcacgacat agactacacc tcatggaaaa attgctgata actggaatca ctgaaaccca
    37501 gcagcatagg cttaccaggc gcgcagagca atggacgttt gataattgca ggcatttcag
    37561 tcattaatgc cgccgcagag gccgcatcgg tgattttatt gcgggtggtt tcgtccagtt
    37621 tacgccaggt tgtaccacgg gtgttgagta acgcttccca gcctaattcg ttgataaaac
    37681 cgttcaataa ttcgctgtcc agcccatcga cgcggtaatc atgaaaacga tagtcgatgt
    37741 tattggcttc cagccaacgg cgagcctttt taatggtgtc acaatttttg atgccgtaaa
    37801 gtgtaaccat aatgaatcct ttttc
//
LOCUS       DAPGOF010000025        37424 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.025, whole genome shotgun sequence.
ACCESSION   DAPGOF010000025 DAPGOF010000000
VERSION     DAPGOF010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.025"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            127..1056
                     /gene="paaA"
                     /locus_tag="RHV13_001120"
     CDS             127..1056
                     /gene="paaA"
                     /locus_tag="RHV13_001120"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit A"
                     /protein_id="HDV1240675.1"
                     /translation="MTQEERFEQRIAQETAIEPQDWMPDAYRKTLIRQIGQHAHSEIV
                     GMLPEGNWITRAPTLRRKAILLAKVQDEAGHGLYLYSAAETLGCAREDIYQKMLDGRM
                     KYSSIFNYPTLSWADIGVIGWLVDGAAIVNQVALCRTSYGPYARAMVKICKEESFHQR
                     QGFEACMALAQGSKAQKQMLQDAINRFWWPALMMFGPNDDNSPNSARSLAWKIKRFTN
                     DELRQRFVDNTVPQVEMLGMTVPDPDLHFDTESGHYRFGEIDWQEFNEVINGRGICNQ
                     ERLDAKRKAWEEGTWVREAALAHAQKQHARKVA"
     gene            1068..1355
                     /gene="paaB"
                     /locus_tag="RHV13_001121"
     CDS             1068..1355
                     /gene="paaB"
                     /locus_tag="RHV13_001121"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit B"
                     /protein_id="HDV1240676.1"
                     /translation="MSNVYWPLYEVFVRGKQGLSHRHVGSLHAADERMALENARDAYT
                     RRSEGCSIWVVKASEIVASQPEERGEFFDPAESKVYRHPTFYTIPDGIEHM"
     gene            1364..2110
                     /gene="paaC"
                     /locus_tag="RHV13_001122"
     CDS             1364..2110
                     /gene="paaC"
                     /locus_tag="RHV13_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaC"
                     /protein_id="HDV1240677.1"
                     /translation="MNQLTAYTLRLGDNCLVLSQRLGEWCGHAPELEIDLALANIGLD
                     LLGQARNFLSYAAELAGEGDEDTLAFTRDERQFSNLLLVEQPNGNFADTIVRQYFIDA
                     WHVALFTRLMESREPQLAAISAKAIKEARYHLRFSRGWLERLGNGTDVSGQKMQQAID
                     KLWRFTAELFDADEIDIALSEEGIAVDPRTLRAAWEAEVFAGINEATLNVPQEQAYRT
                     GGKKGLHTEHLGPMLAEMQYLQRVLPGQQW"
     gene            2125..2622
                     /gene="paaD"
                     /locus_tag="RHV13_001123"
     CDS             2125..2622
                     /gene="paaD"
                     /locus_tag="RHV13_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate degradation protein PaaD"
                     /protein_id="HDV1240678.1"
                     /translation="MQRLATIAPPQVHEIWALLSQIPDPEIPVLTITDLGMVRNVTQM
                     GEGWVIGFTPTYSGCPATEHLIGAIREAMTTHGFTPVQVVLQLDPAWTTDWMTPDARE
                     RLRQYGISPPAGHSCHAHLPPEVRCPRCASVHTTRISEFGSTACKALYRCDSCREPFD
                     YFKCI"
     gene            2630..3700
                     /gene="paaK"
                     /locus_tag="RHV13_001124"
     CDS             2630..3700
                     /gene="paaK"
                     /locus_tag="RHV13_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase/reductase subunit
                     PaaK"
                     /protein_id="HDV1240679.1"
                     /translation="MTTFHSLTVAKVEPETRDAVTITFAVPQPLQEAYRFRPGQHLTL
                     KASFDGEELRRCYSICRSYLPGEISVAVKAIEGGRFSRYAREHIRQGMTLEVMVPQGH
                     FGYQPQAERQGRYLAIAAGSGITPMLAIIATTLQTESESQFTLIYGNRTSQSMMFRQA
                     LADLKDKYPQRLQLLCIFSQETLDSDLLHGRIDGEKLQSLGASLINFRLYDEAFICGP
                     AVMMDDAETALKALGMPEKAIHLERFNTPGTRVKRSVNVQSDGQKVTVRQDGRDREIV
                     LNADDESILDAALRQGADLPYACKGGVCATCKCKVLRGKVAMETNYSLEPDELAAGYV
                     LSCQALPLTSDVVVDFDAKGMA"
     gene            3697..4464
                     /locus_tag="RHV13_001125"
     CDS             3697..4464
                     /locus_tag="RHV13_001125"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dehydroadipyl-CoA hydratase"
                     /protein_id="HDV1240680.1"
                     /translation="MSELIVSRQQRVLLLTLNRPAARNALNNALLTQLVNELEAAAID
                     TSISVCVITGNARFFAAGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGY
                     ALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSG
                     ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQ
                     AGLAQERQLFTLLAATEDRHEGISAFLQKRTPDFKGR"
     gene            4464..5252
                     /gene="paaB"
                     /locus_tag="RHV13_001126"
     CDS             4464..5252
                     /gene="paaB"
                     /locus_tag="RHV13_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase"
                     /protein_id="HDV1240681.1"
                     /translation="MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVER
                     DDTIRCLLITGAGRGFCAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRLAKLPKPV
                     ICAVNGVAAGAGATLALGCDIVIAARSAKFVMAFSKLGLVPDCGGTWLLPRVAGRARA
                     MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINS
                     AETNTLDTQLDLERDYQRLAGRSADYREGVSAFLAKRSPQFTGK"
     gene            5254..6681
                     /gene="paaC"
                     /locus_tag="RHV13_001127"
     CDS             5254..6681
                     /gene="paaC"
                     /locus_tag="RHV13_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase PaaC"
                     /protein_id="HDV1240682.1"
                     /translation="MMINVQTVAVIGSGTMGAGIAEVAASHGHQVLLYDISAEALTRA
                     IDGIHARLNSRVTRGKLTAETCERTLKRLIPVTDIHALAAANLVIEAASERLEVKKAL
                     FAQLAEVCPPQTLLTTNTSSISITAIAAEVKNPERVAGLHFFNPAPVMKLVEVVSGLA
                     TAAEVVEQLCELTLSWGKQPVRCHSTPGFIVNRVARPYYSEAWRALEEQVATPEVIDA
                     ALRDGAGFPMGPLELTDLIGQDVNFAVTCSVFNAFWQERRFLPSLVQQELVIGGRLGK
                     KSGLGVYDWRAEREAVVGLEAVSDSFSPMKVEKKSDGVTEIDDVLLIETQGETAQALA
                     IRLARPVVVVDKMAGKVVTIAAAAVNPDSATRKAIYYLQQQGKTVLQIADYPGMLIWR
                     TVAMIINEALDALQKGVASEQDIDTAMRLGVNYPYGPLAWGAQLGWQRILRLLENLQH
                     HYGEERYRPCSLLRQRALLESGYES"
     gene            6671..7093
                     /gene="paaI"
                     /locus_tag="RHV13_001128"
     CDS             6671..7093
                     /gene="paaI"
                     /locus_tag="RHV13_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HDV1240683.1"
                     /translation="MSHKAWQNAHAMYENDACAKALGIDIISMDEGFAVVTMTVTAQM
                     LNGHQSCHGGQLFSLADTAFAYACNSQGLAAVASACTIDFLRPGFAGDTLTATAQVRH
                     QGKQTGVYDIEIVNQQQKTVALFRGKSHRIGGTITGEA"
     gene            7093..8298
                     /gene="paaJ"
                     /locus_tag="RHV13_001129"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
     CDS             7093..8298
                     /gene="paaJ"
                     /locus_tag="RHV13_001129"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase"
                     /protein_id="HDV1240684.1"
                     /translation="MREAFICDGIRTPIGRYGGGLSGVRADDLAAIPLRELLVRNPRL
                     DAECIDDVILGCANQAGEDNRNVARMATLLAGLPQSVSGTTINRLCGSGLDALGFAAR
                     AIKAGDGDLLIAGGVESMSRAPFVMGKAASAFSRQAEMFDTTIGWRFVNPLMAQQFGT
                     DSMPETAENVAELLKISREDQDSFALRSQQRTAKAQSSGILAEEIVPVVLKNKKGVVT
                     EIQHDEHLRPETTLEQLRGLKAPFRANGVITAGNASGVNDGAAALIIASEQMAAAQGL
                     TPRARIVAMATAGVEPRLMGLGPVPATRRVLERAGLSIHDMDVIELNEAFAAQALGVL
                     RELGLPDDAPHVNPNGGAIALGHPLGMSGARLALAASHELHRRNGRYALCTMCIGVGQ
                     GIAMILERV"
     gene            8325..9638
                     /gene="paaF"
                     /locus_tag="RHV13_001130"
     CDS             8325..9638
                     /gene="paaF"
                     /locus_tag="RHV13_001130"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HDV1240685.1"
                     /translation="MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKF
                     DAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTV
                     VGYTQNDIDNWANIVARSLRAAGGTPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
                     MSGGQTEKQAQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPW
                     TQAMRKEIERRLGITALDIYGLSEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPHDG
                     TPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRRMDRISGRSDDMLII
                     RGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQV
                     CHQLRHRIKSMVGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA"
     gene            9739..10689
                     /gene="paaX"
                     /locus_tag="RHV13_001131"
     CDS             9739..10689
                     /gene="paaX"
                     /locus_tag="RHV13_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HDV1240686.1"
                     /translation="MSKLDTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLA
                     ALLEGLGFGERFVRTALFRLNKEGWLDVSRIGRRSFYSLSDKGLRLTRRAESKIYRAE
                     QPAWDGKWLLLLSEGLDKATLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEA
                     GVADNVICFEAQIPLALSRAALRARVEECWHLTEQNAMYETFIQSFRPLMPLLKETAD
                     ELTPERAFHIQLLLIHFYRRVVLKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAAL
                     AFVSEKGETSVGELPAPGSLYFQRFGGLNIEQEALCQFIR"
     gene            10671..11261
                     /gene="paaY"
                     /locus_tag="RHV13_001132"
     CDS             10671..11261
                     /gene="paaY"
                     /locus_tag="RHV13_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HDV1240687.1"
                     /translation="MPIYQIDGLTPVVPEESFVHPTAVLIGDVILGKGVYVGPNASLR
                     GDFGRIVVKDGANIQDNCVMHGFPEQDTVVEEDGHIGHSAILHGCIIRRNALVGMNAV
                     VMDGAVIGENSIVGASTFVKAKAEMPANYLIVGSPAKAIRELSEQEQAWKKQGTHEYQ
                     VLVTRCKQTLHQVEPLREVESGRKRLVFDENLRPKQ"
     gene            11504..12364
                     /gene="pdxI"
                     /locus_tag="RHV13_001133"
     CDS             11504..12364
                     /gene="pdxI"
                     /locus_tag="RHV13_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HDV1240688.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSSA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKITEIVCVQNEYNIAHRADDAMIDALTREGIAYVPFFPLGGFTPL
                     QSATLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEDV
                     LSTLDSISRE"
     gene            12428..13006
                     /locus_tag="RHV13_001134"
                     /pseudo
     CDS             12428..13006
                     /locus_tag="RHV13_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314703.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13184..14101
                     /locus_tag="RHV13_001135"
     CDS             13184..14101
                     /locus_tag="RHV13_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDV1240689.1"
                     /translation="MLDEGNVAAFEGRDIATFDLAIMRTLPRLKGISANLRKQLIVTL
                     RIRTWWGIVICFSLVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYLVIPLNY
                     LLIGFNCFELFLLFIPLTGFLILATWRVFVGDPSGFLHIVSAIFWGWIMTVFALSHTA
                     WLLMLPTINIQGGALLVLFLLALTGSNNIAQYLWGKSCGRRKVVPKVSPGKTLEGLVG
                     GVITTMIASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLL
                     PGHGGLLDRIDSLIFTATVFFYFIRYCCY"
     gene            14117..15874
                     /locus_tag="RHV13_001136"
     CDS             14117..15874
                     /locus_tag="RHV13_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HDV1240690.1"
                     /translation="MENSRIPGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKTSGPRGYSPSLARSVRDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLLLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDMLGEENRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGT
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPGNEQVKNSLAGLAN
                     AIEPGGILLYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            15864..17180
                     /locus_tag="RHV13_001137"
     CDS             15864..17180
                     /locus_tag="RHV13_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HDV1240691.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFICSSTFEQRRLVHRLILATVMACCG
                     FFLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGACACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPCRIGMWLSMRWFCLRLEPVSKMTADVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPILDLMVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVNEAISYIRARRPQIVLTDEHKAMLRLWENR"
     gene            complement(17234..17839)
                     /gene="azoR"
                     /locus_tag="RHV13_001138"
     CDS             complement(17234..17839)
                     /gene="azoR"
                     /locus_tag="RHV13_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HDV1240692.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAYDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            18040..21942
                     /gene="hrpA"
                     /locus_tag="RHV13_001139"
     CDS             18040..21942
                     /gene="hrpA"
                     /locus_tag="RHV13_001139"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HDV1240693.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAVGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSDQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVGEWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEENGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDKLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            22214..23014
                     /locus_tag="RHV13_001140"
     CDS             22214..23014
                     /locus_tag="RHV13_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDV1240694.1"
                     /translation="MNITPFPTLSTATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFVNPLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIQAWQTLKHDAVLIEAMESRSLR"
     gene            23211..24650
                     /gene="aldA"
                     /locus_tag="RHV13_001141"
     CDS             23211..24650
                     /gene="aldA"
                     /locus_tag="RHV13_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HDV1240695.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(24692..25693)
                     /gene="gap"
                     /locus_tag="RHV13_001142"
     CDS             complement(24692..25693)
                     /gene="gap"
                     /locus_tag="RHV13_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDV1240696.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVNGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            25882..26412
                     /gene="cybB"
                     /locus_tag="RHV13_001143"
     CDS             25882..26412
                     /gene="cybB"
                     /locus_tag="RHV13_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HDV1240697.1"
                     /translation="MENKYSRLQIGIHWLVFLLVIAAYCAMEFRGFFPRSERPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            26657..26830
                     /locus_tag="RHV13_001144"
     CDS             26657..26830
                     /locus_tag="RHV13_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1240698.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(26902..27051)
                     /gene="hokB"
                     /locus_tag="RHV13_001145"
     CDS             complement(26902..27051)
                     /gene="hokB"
                     /locus_tag="RHV13_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HDV1240699.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            27450..29090
                     /gene="trg"
                     /locus_tag="RHV13_001146"
     CDS             27450..29090
                     /gene="trg"
                     /locus_tag="RHV13_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HDV1240700.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAALRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLNKHSVSAEPRGAGEQVSFATV"
     gene            complement(29128..30051)
                     /locus_tag="RHV13_001147"
     CDS             complement(29128..30051)
                     /locus_tag="RHV13_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV1240701.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            30268..31611
                     /locus_tag="RHV13_001148"
     CDS             30268..31611
                     /locus_tag="RHV13_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDV1240702.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDPSLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVMAQPITYEDFLPVS
                     AAGIFQSNLGNETQARSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            31836..33491
                     /gene="opgD"
                     /locus_tag="RHV13_001149"
                     /gene_synonym="mdoD"
     CDS             31836..33491
                     /gene="opgD"
                     /locus_tag="RHV13_001149"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HDV1240703.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            33631..33855
                     /locus_tag="RHV13_001150"
     CDS             33631..33855
                     /locus_tag="RHV13_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HDV1240704.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            33918..34454
                     /gene="rimL"
                     /locus_tag="RHV13_001151"
     CDS             33918..34454
                     /gene="rimL"
                     /locus_tag="RHV13_001151"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HDV1240705.1"
                     /translation="MTETIKVSESLELHAVAENHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(34449..35429)
                     /locus_tag="RHV13_001152"
     CDS             complement(34449..35429)
                     /locus_tag="RHV13_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HDV1240706.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVCGQCRIFGHALINQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVIRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            35553..36545
                     /gene="tehA"
                     /locus_tag="RHV13_001153"
     CDS             35553..36545
                     /gene="tehA"
                     /locus_tag="RHV13_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HDV1240707.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFIARLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            36542..37135
                     /gene="tehB"
                     /locus_tag="RHV13_001154"
     CDS             36542..37135
                     /gene="tehB"
                     /locus_tag="RHV13_001154"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HDV1240708.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILSI
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EDWEMVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
BASE COUNT         9191 a         8780 c        10028 g         9425 t
ORIGIN      
        1 cgaaatccgt cacagttcaa acatacaaaa tttgtgattt cagttaacca ttgtgtaact
       61 ttcataaaac aacgtgattc gtgtttttaa ttaattcacg aaaactggaa tcgtaaaggt
      121 gatgacgtga cccaagaaga acgctttgag caacggatag cccaggaaac ggctatcgag
      181 ccgcaggact ggatgcccga tgcttaccga aagacactga tccgccagat tgggcagcat
      241 gcgcactccg agattgttgg tatgttgcct gaaggtaact ggatcactcg tgcaccaacc
      301 ttgcggcgta aagccattct gttggccaaa gtgcaggatg aagccggtca tggtctgtat
      361 ctctatagcg ccgccgaaac actgggctgc gccagagaag acatctacca aaaaatgctc
      421 gacggacgga tgaaatattc ctccatcttt aattatccga cattgagttg ggctgatatc
      481 ggtgttatcg gctggctggt ggatggcgca gcgatcgtca atcaggtggc gttatgccgc
      541 acttcttatg gcccgtatgc cagagcaatg gtgaaaatct gcaaagaaga gagttttcac
      601 cagcgtcagg ggtttgaagc ctgcatggca ctggcgcagg gcagcaaagc tcaaaagcag
      661 atgttgcaag acgccattaa ccgtttctgg tggccagcct taatgatgtt cgggccaaac
      721 gatgataact cgccaaacag cgccagaagt ctcgcctgga aaatcaaacg tttcaccaac
      781 gacgaactcc gccagcgttt cgtggacaac accgttccac aggttgaaat gctcggtatg
      841 accgttcctg acccggatct gcattttgac actgaaagcg gtcactaccg ctttggtgag
      901 atcgactggc aggagtttaa cgaagtaatt aacggtcgcg gaatttgtaa tcaggagagg
      961 ctcgacgcca aacgtaaagc ctgggaagaa ggtacctggg tacgggaagc agcgctggcc
     1021 catgcacaaa aacaacatgc ccgtaaggtc gcataaggag attcaaaatg agtaatgttt
     1081 actggccgtt atacgaagtt ttcgtgcgtg gcaaacaggg cttatcacac cgccatgttg
     1141 gcagtttaca tgctgccgat gagcggatgg cactggaaaa tgcccgtgat gcttacaccc
     1201 gtcgtagcga aggatgttca atttgggtgg tgaaggcgag tgaaattgtt gcctcgcaac
     1261 cggaagaacg cggtgaattt tttgatccgg ctgaaagcaa ggtctatcgc catccaacgt
     1321 tttacaccat ccctgatggc attgagcaca tgtgaggtcg gaaatgaatc agttaacggc
     1381 ttacaccttg cgcctgggcg ataactgtct ggtgctctcc cagcggttgg gggaatggtg
     1441 cggtcacgca ccggaactgg aaatcgatct cgcactggca aacattggcc tcgatttatt
     1501 aggtcaggca cgtaacttct tatcgtatgc cgctgaatta gcgggagaag gtgatgaaga
     1561 taccctggcc ttcacccgag acgagcgcca gttcagcaac ttattgttgg ttgaacaacc
     1621 aaacggcaat tttgccgaca ccattgtacg ccagtatttc atcgatgcat ggcatgtggc
     1681 gctctttacc cgtctgatgg aaagccgcga gccgcaactg gcggcgattt ctgccaaagc
     1741 aattaaagaa gcgcgttatc acctgcgttt tagtcgtggt tggctggagc gactgggcaa
     1801 tggtactgac gtatcagggc aaaagatgca gcaggccatc gacaagttgt ggcgttttac
     1861 cgccgaactg ttcgatgccg acgagattga tattgcactg agtgaagagg gtattgcggt
     1921 tgatccacgc actttacgcg cagcgtggga agccgaagtt tttgccggaa tcaacgaagc
     1981 cacattgaac gtaccgcaag agcaggcgta tcgcactggc ggtaaaaaag gactgcatac
     2041 cgaacacctt ggacccatgc tggcagaaat gcagtatctc cagcgtgtct tgcccggtca
     2101 gcaatggtaa cagaggagat gggtatgcaa cgtctggcca ccattgcacc gccacaggtt
     2161 catgagatat gggcgctact cagccagatc ccggacccgg agatcccggt gctgaccatt
     2221 accgatttag gcatggtgcg taatgtgaca cagatgggag aaggatgggt gatcggcttt
     2281 acgccgactt attccggttg tccggcaacg gaacatttga ttggcgcgat acgtgaggca
     2341 atgacaaccc acgggtttac ccccgttcag gttgtgctgc aactcgaccc ggcatggacc
     2401 accgactgga tgacccccga tgcccgtgag cgtctgcgac agtatggaat tagcccgccc
     2461 gccggacaca gttgccatgc ccatttgccg ccagaagtac gttgcccgcg ttgcgccagc
     2521 gtccatacca cacgtatcag tgaatttggt tccacggcct gcaaagcatt gtatcgctgc
     2581 gatagttgcc gcgaaccttt cgattatttc aaatgtattt gaggatgcca tgacaacgtt
     2641 tcattcctta acggtggcaa aagtggagcc ggaaacccgt gatgcggtga ccattacctt
     2701 tgcggtgccc cagcctttgc aggaggcgta tcgctttcgc cctggtcaac atttgacctt
     2761 aaaagccagc tttgatggtg aagaattacg ccgttgttac tccatttgcc gcagctacct
     2821 gcctggcgaa attagtgtgg cggtgaaagc cattgaaggc ggacgtttct cccgctatgc
     2881 ccgcgaacac atccgccagg gtatgacgct ggaagtcatg gtgccgcagg ggcatttcgg
     2941 ctatcagccg caggccgaac gtcagggccg ctatctggca attgcagcag gatcaggtat
     3001 tacgccaatg ctggcgatta tcgccaccac tttacaaacc gagtctgaaa gtcagttcac
     3061 cctgatctac ggtaaccgta ccagccagag catgatgttt cgccaggcac tggcagacct
     3121 gaaagacaaa tatcctcagc gtttacagtt gttgtgcatt ttcagtcagg aaactctcga
     3181 cagcgatctg cttcacgggc gtattgacgg tgaaaaatta cagtcacttg gggcctcgct
     3241 cattaatttt cgtctttatg atgaagcgtt tatttgtggt ccggcggtga tgatggatga
     3301 cgcggaaacc gccttaaaag cactgggaat gccagaaaaa gcgattcatc tggagcggtt
     3361 taatacgcct ggcacgcgcg tcaaacgtag cgttaacgtg caaagtgacg gacaaaaagt
     3421 gactgtacgt caggatgggc gggatcggga aatcgtgctt aatgccgacg atgaaagcat
     3481 tctcgatgcg gcattgcgcc agggggcgga tctgccctat gcctgcaaag gcggcgtctg
     3541 tgcgacctgc aaatgcaaag tgctgcgtgg caaagtggcg atggaaacca attacagtct
     3601 ggaaccggat gaactggccg caggctatgt gttgagttgc caggcactgc cgctgaccag
     3661 cgatgtggtg gttgactttg acgcgaaggg gatggcatga gcgaactgat cgtcagccgt
     3721 cagcaacgag tattgttgct gacccttaac cgtcccgccg cacgtaatgc gctgaataat
     3781 gccctgctga cgcaactggt aaatgagctg gaagctgcgg ctatcgatac cagcatttcg
     3841 gtctgtgtga ttaccggtaa tgcacgcttt tttgccgccg gtgccgatct caacgaaatg
     3901 gcagaaaaag atctcgcggc caccttaaac gatacacgtc cgcagctatg ggcgcgattg
     3961 caggccttta acaaaccact catcgcagcc gtcaatggtt acgcgcttgg tgcaggttgc
     4021 gaactggcat tgttgtgcga tgtggtggtt gccggagaga acgcgcgttt tgggttgccg
     4081 gaaatcactc tcggcatcat gccaggcgca ggcggaacgc aacgtttaat ccgtagtgtt
     4141 ggcaaatcgt tagccagcaa aatggtgctg agcggagaaa gtatcaccgc ccagcaagcg
     4201 cagcaggccg ggctggttag cgacgtcttc cccagcgatt taaccctcga atacgcctta
     4261 cagctggcat cgaaaatggc acgtcactcg ccgctggcct tacaagcggc aaagcaagcg
     4321 ctgcgccagt cgcaggaagt ggctttgcaa gccggacttg cccaggagcg gcagttattc
     4381 accttgctgg cggcaacaga agatcgtcat gaaggcatct ccgctttctt acaaaaacgc
     4441 acgcccgact ttaaaggacg ctaatgatgg aattcatcct cagtcatgta gaaaagggcg
     4501 tgatgacact aacgctcaac cgcccggaac gcctgaacag ttttaatgat gagatgcatg
     4561 cacaactggc agagtgcctg aaacaggtcg agcgcgacga cactatccgt tgcctgttaa
     4621 ttacaggtgc cggacgtgga ttttgtgctg gtcaggatct taacgatcgt aatgttgatc
     4681 ccacaggccc cgcaccggat ttagggatgt cagtggagcg tttctataac ccgctggtac
     4741 gtcgcttggc aaaactgcca aaaccggtga tctgtgcagt caatggcgtg gcggcagggg
     4801 ctggcgcaac actggcactg ggttgcgaca tcgttattgc tgcccgttca gcaaaattcg
     4861 tcatggcttt tagtaagtta ggcttagtac ccgattgcgg tggaacctgg ttactgccac
     4921 gcgttgctgg gcgagcgcgc gccatggggc tggcactgct ggggaatcaa ctgagtgctg
     4981 aacaggcgca cgaatggggg atgatctggc aggttgttga tgatgaaacg ctggcagata
     5041 ccgcgcaaca gctggcgcgg catctggcga cacaaccgac atttggtctt ggacttatca
     5101 agcaagcgat aaatagcgct gaaaccaata cgcttgatac gcaactggat ctggaacgcg
     5161 actatcagcg acttgccgga cggagcgcag attatcgtga aggtgtcagt gcgttcctgg
     5221 ctaaacgctc accgcagttc acggggaaat agcatgatga taaatgtgca aactgtggca
     5281 gtgattggga gcggcaccat gggggcaggc attgctgaag ttgctgccag tcatggacac
     5341 caggttttac tgtatgacat ttctgctgaa gcgctgaccc gcgcaatcga cgggatacac
     5401 gcgcggctaa attcacgcgt gacgcgggga aaactgactg ctgaaacctg tgaacgcaca
     5461 ttgaaacgcc tgatcccggt gaccgatatt cacgcgctgg cagctgcgaa tctggttatt
     5521 gaagcggcgt ctgaacgtct ggaagtcaaa aaagcgctct ttgcacagct ggcggaagtt
     5581 tgcccgccac aaacgctatt gaccactaac acttcgtcaa tctctataac cgcgattgct
     5641 gcggaggtaa aaaatcctga acgtgttgcg gggctgcatt tttttaaccc ggcaccggtg
     5701 atgaagttgg tggaggtggt cagtgggctg gcaacggcag cggaagttgt tgagcagttg
     5761 tgtgaactga cattgagttg gggtaagcag cctgtgcgct gtcattcgac tcctggattt
     5821 atcgttaacc gtgttgcgcg tccttattat tccgaggcct ggcgggcact ggaagagcag
     5881 gttgctacac cagaagtgat tgacgctgca cttcgcgatg gcgctggttt cccgatgggg
     5941 ccgctggaat taaccgatct gattggtcag gacgtcaatt ttgctgtcac ctgttcggtg
     6001 tttaacgctt tctggcagga gcgtcgtttt ttaccttcgc tggtgcaaca ggaactggtg
     6061 attggtggac ggttgggcaa gaaaagtggg ctgggcgtgt acgactggcg cgcggaacgt
     6121 gaggcagttg ttggcctgga agcggtaagc gacagtttta gcccaatgaa agtagaaaag
     6181 aaaagtgacg gtgtcacaga aattgacgat gttttattga ttgagacaca aggcgagacg
     6241 gcacaggcgc tggcaatacg actggcacgc ccggtggtag tagtcgacaa aatggcgggc
     6301 aaggtggtga ccattgctgc ggcagcggtg aacccggact cagcgacccg caaggccatt
     6361 tattacctgc aacagcaggg caaaacagtg ctgcaaattg cagattaccc aggaatgctg
     6421 atttggcgaa cggtagcaat gatcatcaat gaagcccttg atgcgctgca aaaaggcgtg
     6481 gcctctgaac aggatatcga taccgccatg cgtcttgggg tgaattatcc atatggcccg
     6541 cttgcctggg gagcgcaact tggctggcag cgaatattaa ggctcctgga aaatctacaa
     6601 catcactatg gcgaagaacg ctatcgccca tgttcattgc tgcgccaacg ggcgcttctg
     6661 gagagcggtt atgagtcata aggcctggca aaatgcccat gcaatgtatg agaacgatgc
     6721 ctgcgccaaa gcgcttggca tcgacattat ctcaatggat gaaggctttg ctgtagtgac
     6781 catgaccgtc actgcacaaa tgcttaatgg tcatcaaagt tgccacggcg ggcagctatt
     6841 ttcactggct gatactgcct ttgcctacgc ctgcaatagc caggggctgg cagccgtcgc
     6901 ttctgcctgc acgattgatt ttttgcgtcc aggctttgcc ggagacacct taactgctac
     6961 tgcgcaggta cgtcatcagg gcaagcaaac cggtgtttac gacatcgaaa ttgttaacca
     7021 acaacaaaaa acggttgcgc tgtttcgcgg taaatctcac cgcatcggcg gcaccattac
     7081 aggagaagcc tgatgcgtga agcctttatt tgtgacggaa ttcgtacgcc aattggtcgc
     7141 tacggtgggg gattatcagg tgttcgggct gatgatctgg ctgctatccc tttgcgggaa
     7201 ctgctggtgc gaaacccgcg tctcgatgcg gaatgtatcg atgatgtgat cctcggctgt
     7261 gctaatcagg cgggagaaga taaccgtaac gtagcccgga tggcgacttt actggcgggg
     7321 ctgccgcaga gtgtttccgg cacaaccatt aaccgcttgt gtggttccgg gctggacgca
     7381 ctggggtttg ccgcacgggc gattaaagcg ggcgatggcg acttgctgat cgccggtggc
     7441 gtggaatcaa tgtcacgagc gccgtttgtt atgggcaagg cagccagtgc attttctcgt
     7501 caggctgaga tgttcgatac cactattggc tggcgatttg tgaacccgct catggctcaa
     7561 caatttggaa ctgacagcat gccggaaacg gcagaaaatg tagctgaact gttaaaaatc
     7621 tcacgagaag atcaagatag ttttgcacta cgcagtcagc aacgtacggc aaaagcgcaa
     7681 tcctcaggca ttctggctga ggagattgtt ccggttgtgt tgaaaaacaa gaaaggtgtt
     7741 gtaacagaaa tacaacatga tgagcatctg cgcccggaaa cgacactgga acagttacgt
     7801 gggctgaaag caccgtttcg tgccaatggg gtgatcaccg caggcaatgc ctccggggtg
     7861 aatgacggag ccgctgcgtt gattattgcc agtgaacaga tggcagcagc gcaaggactg
     7921 acaccgcgag cgcgtatcgt agccatggca accgccgggg tggaaccgcg cctgatgggg
     7981 cttggtccgg tgcctgcaac tcgccgggtg ctggaacgcg cggggctgag tattcacgat
     8041 atggacgtga ttgaactgaa cgaagcattc gcggcccagg cgttgggtgt actacgcgaa
     8101 ttggggctgc ctgatgatgc cccacatgtt aaccccaacg gaggcgctat cgccttaggc
     8161 catccgttgg gaatgagtgg tgcccgcctg gcactggctg ccagccatga gctgcatcgg
     8221 cgtaacggtc gttacgcatt gtgcaccatg tgcatcggtg tcggtcaggg catcgccatg
     8281 attctggagc gtgtttgagc atatcaacct gcgagtaccc tacaatgata accaatacaa
     8341 agcttgaccc gattgaaacc gcgtctgttg atgagttaca ggcgttgcaa acacagcgtc
     8401 tgaaatggac gctcaaacac gcgtatgaaa atgtcccgat gtatcggcgc aaattcgacg
     8461 cagcaggcgt acatcctgat gatttcaggg aactttcaga cctgcgtaaa tttccctgta
     8521 ccaccaaaca ggatctgcgc gacaactatc ctttcgacac ctttgccgtg ccgatggaac
     8581 aagtggtgcg cattcatgct tcttcgggaa ccacaggtaa accgacagtt gtcggctata
     8641 cgcaaaacga tattgataac tgggccaata ttgtagcgcg ttcgttgcgt gcggcgggtg
     8701 gcacgcccaa agacaaaatt catgtcgcct atggttacgg gctgtttact ggtgggctgg
     8761 gtgcgcacta tggtgccgaa cgtctgggcg ctacggtgat cccgatgtct ggcgggcaga
     8821 cagaaaaaca agcgcaactg atccgcgatt ttcaaccaga tatgatcatg gttacgccat
     8881 cttattgcct taacctgatt gaagagctgg agcggcagtt gggcggtgat gccagcggtt
     8941 gctcgctgcg ggttggcgtg tttggtgctg agccgtggac acaggccatg cgtaaagaga
     9001 ttgaacggcg tctggggatc accgcactgg atatttatgg cctgtcagaa gtgatggggc
     9061 cgggggtggc gatggagtgt ctggaaacta ccgacggccc aactatttgg gaagatcatt
     9121 tctatcctga aattgttaat cctcatgacg gcacaccgct tgccgatggc gaacatggcg
     9181 aactgttatt caccacgctg accaaagaag cattgccggt cattcgttac cgcacgcgtg
     9241 atttaacccg actgttacca ggaacggcgc ggactatgcg ccgtatggat cgcatcagcg
     9301 gacgtagcga tgacatgttg atcattcgcg gtgtaaatgt cttcccgtca caactggaag
     9361 aagagattgt caaatttgaa catttatcgc cgcattacca actggaggtg aaccgccggg
     9421 ggcatcttga ttcactttct gtgaaagtgg agttgaaaga aagtagcctg acattgacgc
     9481 atgagcagcg ttgccaggtg tgccaccagc tacgccatcg gattaagtcg atggtgggga
     9541 tttctaccga tgtgatgatc gttaactgtg gcagtatccc gcgttcagaa ggcaaggcgt
     9601 gtcgggtgtt tgatctgcgc aatattgttg gtgcctgacg tgtcgtatgg ccctggtggg
     9661 gtaaagcgcc agggccagaa gtcgatacga cctgtgctat gattcataaa tcacaacaat
     9721 aacaacagac tgaatcgaat gagtaaactt gatactttta tccaacatgc tgtaaacgct
     9781 gttccggtca gtggcacatc tttgatctcc tctctgtatg gtgattcgct ttcccatcgt
     9841 ggtggtgaaa tctggctggg tagtctggcg gctttgctgg aagggctggg atttggtgag
     9901 cgtttcgtgc gcaccgcttt gtttcgtctt aataaagaag gctggctgga tgtttcccgc
     9961 atcgggcgac gcagtttcta tagcctcagt gataaaggct tgcgcctgac gcgacgggca
    10021 gaaagtaaaa tttatcgcgc agagcaacct gcatgggatg gtaaatggct cctgttgctc
    10081 tcggaaggtt tagataaagc gacgctggct gatgtcaaaa agcagttgat ctggcaaggt
    10141 tttggcgcac tggcacccag cctgatggca tcgccgtcgc aaaaactggc ggatgtacag
    10201 acacttttgc atgaagcggg tgtggcggat aacgtgattt gttttgaagc gcaaatacca
    10261 ctggcgcttt ctcgtgcagc actgcgtgcc agagtggaag agtgctggca tttaactgaa
    10321 caaaatgcca tgtacgaaac ctttattcag tcattccgcc cgctgatgcc gctgttaaaa
    10381 gagacggcag acgagttaac cccggagcgt gcatttcata ttcagctttt actgatccat
    10441 ttttatcgcc gtgtcgtcct taaagaccca ttgttgccgg aggagttgct tccggctcac
    10501 tgggcagggc atacggcgcg tcagctgtgt atcaacattt atcagcgcgt agcgcctgct
    10561 gctttagcgt tcgttagtga aaaaggtgaa acctcggtcg gtgaactgcc tgcgccggga
    10621 agcctgtatt ttcaacgttt tggcggcttg aatattgaac aggaggcgtt atgccaattt
    10681 atcagataga cggtctgact ccagttgtgc cagaagagag ttttgtccat ccgacagcgg
    10741 tattgatcgg cgatgttatt ctcggcaagg gcgtttacgt tgggccaaat gccagtctgc
    10801 gtggcgattt tggtcgtatc gtggtgaaag atggtgcgaa cattcaggat aattgcgtta
    10861 tgcacggttt tcccgagcag gatactgtcg ttgaagaaga tggacatatt ggtcatagcg
    10921 ctatccttca cggctgcatt atccgccgca atgcattagt gggaatgaac gcggtagtga
    10981 tggacggtgc ggtgattggc gagaacagca ttgttggcgc atccacattt gtgaaagcca
    11041 aagcagaaat gcctgctaat tacctgattg taggcagccc ggcgaaagcg attcgtgaac
    11101 ttagtgagca ggagcaggca tggaaaaagc agggtacgca tgagtatcag gtgctggtga
    11161 cacgctgtaa gcagacgttg catcaagttg agccattgcg ggaagttgaa tccggcagga
    11221 aacgcctggt atttgatgag aatctgcgac caaaacagta acgtccagat actgaacatt
    11281 ctaattttta gcatgtgatc cctaaaccgc aacgctgata caggttgcgg tttattattg
    11341 ccggatgcgg tgcttgatgc gtcttttgct gcctaatgtt ttggtgtctt tcaggataac
    11401 agagcaactc atccagtaat cttgtttacg ccttcgcatt atttatctct tttcgtttct
    11461 atactgattt ttcttaatcc gttttattac agggcagggt gcgatgagca gcaatacatt
    11521 tactctcggt actaaatccg ttaaccgtct tggttatggc gcgatgcaac tggcaggtcc
    11581 tggggttttt ggtcccccaa gagatcgcca cgtcgctata accgtgctgc gtgaggcgct
    11641 ggcattgggc gtcaatcaca ttgataccag cgatttttat ggtccgcacg tcaccaatca
    11701 gattatccgc gaagcgcttt atccttactc tgacgacctg acaattgtca ctaaaattgg
    11761 tgcgcggcgt ggagaggacg cttcctggtt gcccgccttt tcttcggcag agctgcaaaa
    11821 agcggtacac gataatctac gtaatctcgg cctggacgta ctggatgtgg ttaacctgcg
    11881 cgtgatgatg ggggatggtc atggcccagc ggaaggatcg attgaagcca gcctgaccgt
    11941 gctggcagag atgcaacaac aaggcctggt aaaacatatt ggcctgagca acgtcacacc
    12001 gacgcaggtt gcagaggcgc gtaagattac cgaaattgtc tgtgtgcaaa acgaatacaa
    12061 cattgctcat cgtgctgatg acgcaatgat tgatgctttg acccgcgaag gtattgccta
    12121 tgtgccgttc ttcccgctcg ggggctttac gccgctgcaa tccgccacgc tttctgatgt
    12181 tgcagcgagc ctgggtgcaa cacccatgca ggtggcgctg gcgtggctgt tacaacgttc
    12241 accgaatatt ttgctgatcc cagggacgtc ttctgttgcg catttacggg agaatatggc
    12301 tgctgaaaaa ttgcatcttt ctgaggatgt gttgtctacg ttggatagta tttcgcgaga
    12361 ataacgaata tacaaaaggg aaagatgcat ttcccttttg tttctttttt aatggcatgg
    12421 agtgcatatg tttaagccca ggatttgcag ctggataggt ttacttctcc tgtttatgct
    12481 gagtacttcc agtacaggcg gaacttcgat gtgttgcaaa tgcagttgat attgaaccat
    12541 ttttttctgc ggctaccgct gaagataaac aacaagttga acaagctatc aacagtagcg
    12601 tgaatcttgt cccttcggtt tatctgcatc ggactggaaa gtgcatcgtg gcgatttagt
    12661 ggtagaaggt aatatagaga gtaatcaaaa attgattgtt cttggcaatt tgacagtcaa
    12721 aggtaatatt tccactttct ctctttctaa tccatgggtt attctcggta acgtgacggc
    12781 aacgaatata gttacggatt caccactgtt aattaccggt tcgataaatg caagtggact
    12841 ggtatttatc gactcatatt acgataatcc gtctacgatt aaggggagta ttaatgcgcg
    12901 tgggatattt atcaatgaca taattgcacc tgttgttgcg tcttcgacaa atagtgaatt
    12961 catggtccgt gcgagtgaca aaaatgacac tgaaaatgtc aaaaaagcgc tgatgataat
    13021 aaatcctgat gcatattatt ggggtctaat taatgatgaa gatgctctga aagaaatttt
    13081 taagcgaagc aatattcgca tggcagggaa tgtctgtaat cagatgaaaa aagaagcgct
    13141 gtttcgccct aagccttctc ctgagttagt gcaggaattg caaatgctgg atgaaggcaa
    13201 cgttgctgca tttgaaggac gagatattgc gacatttgat cttgccataa tgcgaactct
    13261 cccaaggctt aaaggaattt cagctaacct acgcaaacaa cttattgtta cattacgtat
    13321 tcgtacctgg tggggtatcg tcatttgttt ttcactggtg atttcaggtc cacgctggat
    13381 gacgttgacg ttttttgcgc tgataagttt tctggcattg aaagaatatt gtacgcttat
    13441 atccgtacat tttccgcgtt ggttatattt ggttattcct cttaactatt tgcttatcgg
    13501 ttttaattgc tttgagctgt ttctgttatt tatacctttg actgggtttc tgatattagc
    13561 cacctggcga gtttttgtgg gtgatccctc cggttttctg catatcgtga gcgccatttt
    13621 ttggggctgg ataatgaccg ttttcgcctt gagtcatacc gcctggctat taatgttgcc
    13681 aaccataaat atccagggcg gggcgttact ggtgttattt cttttggcgt taaccgggtc
    13741 aaacaatatt gcacagtatt tatgggggaa atcctgcggc agaagaaaag tggtccccaa
    13801 agtcagcccg ggaaaaacgc tggaaggtct ggtggggggc gtcatcacca ccatgattgc
    13861 gtcactgatt attgggccgt tgctgacacc gctaaataca ttacaggcat tattagcggg
    13921 tttgttaatt ggtattagtg gtttttgcgg cgatgtagtg atgtcagcca tcaaacgaga
    13981 tattggtgtt aaagatagtg gaaaactatt accaggacat ggcggactcc ttgacaggat
    14041 tgactcatta attttcaccg ctacggtatt tttttatttt attcgctact gctgttactg
    14101 aaggaaaata gagaaaatgg aaaattcacg catccctggg gaacattttt ttaccaccag
    14161 tgataataca gcgttgtttt atcggcactg gcccgcttta cagcccgggg cgaaaaaggt
    14221 catcgtctta tttcatcgcg gtcatgaaca ttctggtcgt ctacaacatc tcgttgatga
    14281 actggcgatg ccagatactg ctttttatgc ctgggatgcc cgagggcatg gaaaaacttc
    14341 ggggccgcgt ggttatagcc catctcttgc gcgttcagtg cgggatgtcg atgaatttgt
    14401 ccgttttgct gccagcgaca gccaggtcgg gctggaagag gtggtagtga tcgcgcagag
    14461 cgtcggcgca gtgctggttg ccacatgggt tcatgattat gcgcctgcaa ttcgcgggct
    14521 gctgctggct tctccggcct ttaaggttaa attgtatgtg ccgctggcac gtcctgcgct
    14581 ggcgttatgg catcgtctgc gtggtctgtt ttttattaat tcctatgtga aaggacgcta
    14641 tttgacccac gatcggcaac gggtggcgag tttcaataat gatccgctga tcacaagggc
    14701 gattgccgtc aatatcttgc tagatctcta taaaacgtct gaacgtattg ttagcgatgc
    14761 ggcggcgatt acgctcccca cgcaacttct gatatcaggc gatgactatg tggtgcatcg
    14821 ccaaccgcag attgattttt atcagagatt acgtagccct ctgaaagagc tgcatctgct
    14881 gcctggcttt tatcacgaca tgttgggtga agagaacagg gcgcaggcat ttgaaaaaat
    14941 gcaaagcttt attagtcgtt tatatgctaa caagtcacaa aaatttgatt atcagcatga
    15001 agaccgcact ggaccatcag cggatcgctg gcggctcctt tctggtggac ccgtgccatt
    15061 atcgccggtt gatttggcgt atcgctttat gcgtaaagcg atgaaattgt tcgggacgca
    15121 ctctgcgggc ctgcatctcg gaatgagcac cggctttgat tcaggcagtt cgctggatta
    15181 tgtctatcaa aatcaaccgc aaggtagtaa cgcattcggg cgcttaatcg acaaaatcta
    15241 cctgaacagt gttggctggc gcggtattcg ccagcgcaaa acccatttac aaatactgat
    15301 taaacaagcc gttgccgatc tccacgccaa aggtttagcc gtccgcgtgg ttgacattgc
    15361 cgcagggcat gggcgctatg tactggatgc gctggcaaac gagcctgccg taagcgatat
    15421 tttgttacgt gattacagcg agttaaatgt tgcacagggg caagagatga ttgctcaacg
    15481 gggaatgtct gggcgggtgc gttttgaaca gggcgatgcg tttaacccgg aggaactcag
    15541 cgcgttaact ccgcggccta cgctggcgat tgtctctgga ctgtatgagc tttttcccgg
    15601 aaatgagcag gtaaaaaact cactcgcagg tcttgccaat gccatcgaac cgggcggcat
    15661 tctcctctac accgggcagc cgtggcaccc acaactggag ctgattgccg gggtgttaac
    15721 cagtcataaa gatggtaaac cgtgggtaat gcgcgtgcgt tcgcaagggg agatggattc
    15781 actcgtgcgt gatgccggat ttgataaatg cacacaacgg attgatgagt ggggcatttt
    15841 tacggtttcg atggcggtgc gtcgtgataa ctgaacgtcg gaacgtattg ctacaaggcg
    15901 ctggctggtt attgttgctg gccccgtttt tcttcttcac ctatggatct cttaatcagt
    15961 tcaccgcggt tcaggacctt aacagccatg atatccccag tcaggtattc ggttgggaaa
    16021 cggcgatccc ttttcttccc tggactattg ttccttactg gagtctggat cttttatatg
    16081 gattttcgct gttcatttgt agctcgacat tcgaacaacg ccgacttgtc caccggctta
    16141 ttctggcaac ggtaatggcc tgctgcggtt tttttctcta cccgctgaag tttagtttta
    16201 tccgtcctga agtgagtggg gtgacaggat ggctattttc gcaacttgaa ctgtttgatc
    16261 tgccctataa ccagtctcct tcgctgcata ttattctctg ctggctactt tggcgtcact
    16321 ttcgtcagca tctggctgtg aggtggcgta aagtctgcgg cggatggttt ttactcatcg
    16381 ccatttcgac gctgacaacc tggcagcatc attttattga tgtcatcaca gggctggcgg
    16441 taggtatgtt gattgactgg atggtgcccg tcgaccgtcg ttggaattat cagaaacctg
    16501 atcaacgtcg aatcaaaata gcactgccat atgtcgtagg cgcgtgcgcg tgcattgtgt
    16561 tgatggagct aatgatgatg gttcagttat ggtggtcagt ctggttatgt tggccagtat
    16621 tatcgctact cattattggc cgtgggtacg gtgggcttgg cgcgataaca acagggaaag
    16681 atagtcaggg gaaactcccg cccgccgttt actggctgac attgccctgc cgcatcggga
    16741 tgtggctgtc tatgcgttgg ttttgtcttc gcctggagcc ggtgagcaaa atgactgctg
    16801 atgtttattt aggggcgttt ccacgacata ttccggcaca gaatgcggtt ctggacgtca
    16861 cctttgaatt ccctcgcgga cgagcgacaa aagatcgact ctatttctgt gtaccgatat
    16921 tggatctgat ggttccggaa gagggggaac tccgacaggc cgtggcgatg ctggaaacat
    16981 tacgcgaaga gcaaggcagc gttctggtcc attgtgcatt gggattatcg cgcagtgcgc
    17041 tggtggtggc ggcatggttg ttatgttacg gacactgtaa aaccgttaat gaagcgatta
    17101 gctatattcg agccagacgc ccgcagattg tgttgacaga cgaacacaaa gcgatgctga
    17161 gattgtggga aaacaggtaa gtggattgag atgtggactg aatatctaca gtccacatca
    17221 agaccgtgtc cggttatgca gcaacaatgc tgtcgatggc tgcttttgcg tcagactgtg
    17281 ctttcgctgc catttccgga ccgtatgcga tcccttcggc gaagacaaat ttcacatcgg
    17341 taatgccgat aaagccgagg aacgtggaca gatacggcgt caccaggtcc gttggtccat
    17401 ctttgtggat cccaccgcgg ctggtaataa cgatggcttt tttacccgtt accagacctt
    17461 ccggaccatt ctcggtatag cggaaagtaa cgcctgcgcg tgcaaccagg tcaaaatagt
    17521 ttttcaactg agtcgagatg ttgaagttat acatcggtgc cgcaataacg ataacgtcgt
    17581 aggctttcag ttcggcaatc agctcatcag aaagcgccag cgcttcctgc tgacgcggag
    17641 tcagcggcgc atcgctcgga cgcagagcgc caaccagttc gccatccagt accggaattg
    17701 gatttgcagc caggtcgcga acggtgattt catcagcgga gtgcttttcg cgccattgtt
    17761 caacaaaata atcggacaac tgattagact gagagtaccc tgccaggatg ctggatttaa
    17821 gaactaatac cttgctcatg gtgtttcctt atagatattt gaatgggcga tgccccgttg
    17881 cttgttgaca ctttattcac aatcctgcca cagagatagc gcaataaatc gaagcctatg
    17941 ttcgaattta ttgaacaacg catagaaagc tgcgatgtgg tactctatat ctatcattta
    18001 aaagaaaatt aatcaggcag cctactgccc actaacgtta tgacagaaca acaaaaattg
    18061 acctttactg ccttgcagca gcggctggat tcgctgatgc tgcgtgacag gctgcgtttt
    18121 tctcgccgtc tgcacggtgt gaagaaggtt aaaaatcctg atgcacaaca ggccattttc
    18181 caggagatgg cgaaagagat tgaccaggcg gtagggaaag tcctgctacg tgaagcggca
    18241 cgtccggaaa tcacttatcc ggacaatttg ccggttagtc agaaaaaaca ggacattctc
    18301 gaagcgattc gtgatcacca ggtggtaatc gtcgccgggg aaacgggttc tggtaaaacg
    18361 actcagttac cgaaaatctg tatggagctg gggcgcggga ttaaagggct gatcggccat
    18421 acccagccgc gtcgcctggc ggcaagaacg gtggcgaacc gtattgcgga agagctgaaa
    18481 acggagccgg gcggttgcat cggttacaaa gtgcgtttca gcgatcacgt aagtgataac
    18541 acgatggtca agctgatgac cgacggtatc ctgctggcgg agatccagca agaccgcctg
    18601 ctgatgcagt acgacaccat cattattgac gaagcgcacg aacgcagcct gaatatcgat
    18661 tttctgctcg gctatttgaa agagttgctg ccgcggcgtc ctgacctaaa aatcattatc
    18721 acttccgcga ctatcgaccc ggaacgcttt tcgcgccact ttaataatgc gccgattatt
    18781 gaagtctccg gtcggaccta tccggtggaa gtgcgctatc gcccgattgt tgaagaagcc
    18841 gatgacaccg agcgcgatca gttgcaggcg atttttgacg ccgtagacga actaagccag
    18901 gaaagccccg gcgacattct gatctttatg agcggcgagc gggaaattcg cgataccgcc
    18961 gatgcgctga acaagctgaa cttacgccat accgaaatct tgccgcttta tgcgcggctt
    19021 tcgaatagcg atcagaaccg cgtgttccag tcgcacagcg ggcgacgcat tgtgctggcg
    19081 accaacgtcg cggaaacctc gctgactgta ccggggatta aatacgttat cgaccccggt
    19141 acggcgcgta tcagccgcta cagctatcgc accaaagtgc agcgtttgcc gattgagccc
    19201 atttcgcagg cgtctgccaa tcagcgtaaa ggccgctgtg gtcgtgtgtc cgaagggatc
    19261 tgtattcgtc tctattccga agacgatttc ctttcgcgcc cggagtttac cgatccggag
    19321 attctgcgta ccaacctggc ctcggttatt ttgcagatga ccgcgctggg gttgggcgat
    19381 atcgccgcgt tcccgtttgt agaagcaccg gataaacgca atatccagga tggcgtgcgt
    19441 ctgctcgaag agctgggcgc gatcaccacg gatgaacagg ccagcgccta taaactgaca
    19501 ccgctcggtc gccagctctc gcagttgcct gtcgacccac gtttggcgcg tatggtgctg
    19561 gaagcgcaaa aacatggctg cgtgcgtgag gcgatgatta tcacgtccgc gctctccatt
    19621 caggatccgc gcgagcgtcc gatggataaa cagcaggcgt cggacgaaaa acatcgccgc
    19681 ttccacgaca aagaatccga cttcctcgcg tttgtaaatc tgtggaatta tcttggcgag
    19741 cagcaaaagg cgctttcttc caacgccttc cgtcgcctgt gtcgtaccga ttatctcaac
    19801 tatctgcgcg tgggcgaatg gcaggatatc tacactcagt tgcgccaggt ggtgaaagag
    19861 cttggcattc cggttaacag cgaaccggcg gagtatcgcg aaattcacat cgccttactg
    19921 accggtttgc tttcccatat cggcatgaaa gatgccgata aacaagaata taccggcgca
    19981 cgtaacgcgc gtttctccat cttccccggt tctggtttat tcaaaaaacc gccgaaatgg
    20041 gtaatggtgg cggaactggt agaaaccagc cgcctgtggg gacgtattgc ggcacgcatc
    20101 gatccagaat gggtggaacc cgtcgctcag catttgatta aacgtaccta cagcgaaccg
    20161 cactgggaac gggcgcaggg cgcggtgatg gcaacggaaa aagtcactgt ttatggtttg
    20221 ccgattgttg ccgcgcgcaa ggtcaactac agccagatcg atccggcgtt atgtcgtgaa
    20281 ctctttattc gccacgcgct ggtggaaggt gactggcaga cgcgtcacgc attctttcgt
    20341 gaaaacctga aactgcgtgc cgaagtggaa gagctggaac acaaatcacg tcgccgcgat
    20401 attctggtgg atgacgaaac gctgtttgag ttctacgacc agcgcatcag ccacgatgta
    20461 atctccgctc gtcacttcga cagctggtgg aaaaaagtca gccgcgaaac gcctgatttg
    20521 ctcaactttg aaaagagcat gttgatcaaa gaaggggcgg aaaaaatcag caagctggat
    20581 tatccgaact tctggcatca gggcaatctc aagctgcgtt tgagctatca gtttgagcca
    20641 ggcgcggacg cggacggtgt gaccgtacat attccgctgc cgttacttaa ccaggttgag
    20701 gaaaacgggt ttgaatggca gatccccggt ctgcgccgcg aattggtgat tgctctgatt
    20761 aaatcgttgc cgaaaccggt acgccgtaat tttgtgcctg caccaaacta cgccgaagcg
    20821 tttttaggcc gcgtcacacc gctggagtta ccgttgctcg acagccttga gcgcgagtta
    20881 cggcggatga ccggtgttac cgttgaccgc gaagactggc actgggatca ggtgcccgat
    20941 cacctgaaaa tcaccttccg cgtggtggat gacaaaaaca agaagctaaa agaagggcgc
    21001 tctctacaag atctgaaaga tgcgctgaaa ggcaaagtgc aggaaacgct gtctgcggtg
    21061 gcggatgacg gtatcgagca gagcggctta catatctgga gttttggtca gctgccggaa
    21121 agctacgaac agaagcgtgg caactacaaa gtgaaggcgt ggccagcgct ggtggatgag
    21181 cgcgacagcg tggcgatcaa actgtttgat aatccgctgg aacaaaagca ggcaatgtgg
    21241 aacggtcttc gccgtctact gctgctgaat attccatcgc cgatcaagta tttacatgag
    21301 aagttaccaa acaaagccaa gcttggactg tactttaacc cgtatggcaa agtgctggag
    21361 ctgatcgacg actgtatctc ctgcggtgtg gataaattga tcgacgccaa tggtggcccg
    21421 gtctggacgg aagaaggctt tgctgcgctg catgaaaaag tgcgcgccga actgaatgac
    21481 acggtggtgg atattgcgaa gcaggtcgag caaatcctta cggcggtgtt caatatcaac
    21541 aaacgcctga aggggcgggt ggatatgacg atggcgctgg ggctttctga cattaaagcg
    21601 cagatgggcg ggctggtgta tcgcggtttt gtgactggta acggcttcaa acggctgggc
    21661 gacacgctgc gttatttgca ggcgattgaa aaacggctgg aaaaactggc ggttgatcca
    21721 catcgcgatc gtgcacagat gctgaaagtc gaaaacgtcc agcaggcgtg gcagcaatgg
    21781 atcaacaaac tgccgcctgc acgtcgtgag gatgaagacg tgaaagagat ccgttggatg
    21841 atagaagagc tgcgcgttag ttacttcgct caacaactcg gtacacctta tccgatttca
    21901 gataagcgta ttttgcaggc gatggagcag attagcggtt aacgcggcta tttgcctgat
    21961 aaatataaaa cccggtaagc atttagcgcc gggtttttta ttaattctaa aacggtaagg
    22021 gcaaaaattc aggaattcag aaaaatacaa ttctctgctg caagatgaat aatgtttatc
    22081 tacagcattt ccttaaaaga tatgtcaggc ttgcggagtg gcggttaagg aaatacgact
    22141 tcctcctttc agagtgctcc gcttctcact attatctcac gcagtattct taagggaacg
    22201 ataaggagga accatgaaca ttaccccgtt tccgacgctt tcgacggcaa ctatagatgc
    22261 cataaatgtt atcggacagt ggctggcgca ggatgatttc tccggtgagg tgccgtatca
    22321 ggccgattgc gtcatacttg caggcaatgc ggttatgccg actatagatg cggcatgtaa
    22381 gattgcccgc gatcagcaaa ttcctttact gattagtggt ggtatcggtc actcgacaac
    22441 ttttttgtat agcgccatcg cacagcatcc gcactacaac actatccgca ccactggcag
    22501 agcagaagcg accatcctgg cggatatcgc tcatcagttc tggcacattc cgcatgaaaa
    22561 aatctggatt gaagatcagt caacaaactg cggtgaaaac gcacgcttta gcatcgcgct
    22621 attgaatcag gccgtagaac gagttcatac ggctatcgtt gttcaggacc ccaccatgca
    22681 gcgacgcacg atggcgacgt tccgccgtat gactggggac aatcccgatg caccacgctg
    22741 gttaagttat cccggattcg ttcctcagtt aggaaataac gcagacagtg taatctttgt
    22801 taatccgttg caaggattat ggccggttga gcgttatctc tcactactca ctggcgagct
    22861 gccgcgttta cgcgatgata gcgatggcta cggtccccgc gggcgagatt ttatcgttca
    22921 cgttgatttt ccggcagaag tcattcaggc atggcaaacg cttaaacacg atgcggtgct
    22981 catcgaggcg atggaaagtc gctcgttacg ttaaaaattg cccgtttgtg aaccacttgt
    23041 ttgcaaacgg gcatgactcc tgacttttat ttctgccttt tattcctttt acacttgttt
    23101 ttatgaagcc cttcacagaa ttgtcctttc acgatgccgt ctctctgatg attgatgtta
    23161 attaacaatg tattcaccga aaacaaacat ataaatcaca ggagtcgccc atgtcagtac
    23221 ccgttcaaca tcctatgtat atcgatggac agtttgttac ctggcgtgga gacgcatgga
    23281 ttgatgtggt aaaccctgct acagaggctg tcatttcccg catacccgat ggtcaggccg
    23341 aggatgcccg taaggcaatc gatgcagcag aacgtgcaca accagaatgg gaagcgttgc
    23401 ctgctattga acgcgccagt tggttgcgca aaatctccgc cgggatccgc gaacgcgcca
    23461 gtgaaatcag tgcgctgatt gttgaagaag ggggcaagat ccagcagctg gctgaagtcg
    23521 aagtggcttt tactgccgac tatatcgatt acatggcgga gtgggcacgg cgttacgagg
    23581 gcgagattat tcaaagcgat cgtccaggag aaaatattct tctgtttaaa cgtgcgcttg
    23641 gtgtgactac cggcattctg ccgtggaact tcccgttctt cctcattgca cgcaaaatgg
    23701 ctcccgctct tttgaccggt aataccatcg tcattaaacc cagtgaattt acgccaaaca
    23761 atgcgattgc attcgccaaa atcgtcgatg aaataggcct tccgcgcggc gtgtttaacc
    23821 ttgtactggg gcgtggtgaa accgttgggc aagaactggc gggtaaccca aaggtcgcaa
    23881 tggtcagtat gacaggcagc gtctctgcag gtgagaagat catggcgact gcggcgaaaa
    23941 acatcaccaa agtgtgtctg gaattgggtg gtaaagcacc agctatcgta atggacgatg
    24001 ccgatcttga actggcagtc aaagccatcg ttgattcacg cgtcattaat agtgggcaag
    24061 tgtgtaactg tgcagaacgt gtttatgtac agaaaggcat ttatgatcag ttcgtcaatc
    24121 ggctgggtga agcgatgcag gcggttcaat ttggtaaccc cgctgaacgc aacgacattg
    24181 cgatggggcc gttgattaac gccgcggcgc tggaaagggt cgagcaaaaa gtggcgcgcg
    24241 cagtagaaga aggggcgaga gtggcgttgg gtggcaaagc ggtagagggg aaaggatatt
    24301 attatccgcc gacattgctg ctggatgttc gccaggaaat gtcgattatg catgaggaaa
    24361 cctttggccc ggtgctgcca gttgtcgcat tcgacacgct ggaagatgct atctcaatgg
    24421 ctaatgacag tgattacggc ctgacctcat caatctatac ccaaaatctg aacgtcgcga
    24481 tgaaagccat taaagggctg aagtttggtg aaacttacat caaccgtgaa aacttcgaag
    24541 ctatgcaagg cttccacgcc ggatggcgta aatccggtat tggcggcgca gatggtaaac
    24601 atggtctgca tgaatatctg cagacccagg tggtttattt acagtcttaa tgagtgaaag
    24661 aggcggaggt tttttcctcc gcctgtgcgc gtcagagttt agcgaatttt tcgagggtgc
    24721 gaataagctg cgtgacgaag ccatattcgt tatcgtacca ggcgaccgtt ttcaccagtt
    24781 gtaaatcgcc cacggcggta atttccgttt gcgtggcatc aaacaccgaa ccgaaatggc
    24841 tgccaatgat atcggaagag actatttctt catcggtata accaaatgac tcgttattgg
    24901 tggttgcttg tttaagtgcg ttattcacct cttcggcagt cacttttttt ccgagaatcg
    24961 ataccagttc agtgaccgaa cctgttttca ccggcacgcg ttgcgcatga cctttcagtt
    25021 tgccgctcag ttccgggatc accagaccaa tggcttttgc cgcgcccgta gtgtggggga
    25081 tgatattttc tgccgctgcg cgtgaagcac gtaaatcttt accacgcggg ccatccacca
    25141 gtgactgggt gccagtatag gcatgaatgg tcgtcatcgt gccgacttct atcccgaaac
    25201 tgtcatgcaa ggctttggcc atcggcgcaa ggcagttagt ggtgcatgac gccacggaaa
    25261 caatggtatc gttgccatcc agagtgtcgt cattgacgtt ataaacgata gttttcattt
    25321 caccggcagg ggcggaaatc aacaccttct tcgcgccagc atcgagatgc gcctgcgatt
    25381 tctcggcgga ggtataaaag ccagtacatt cgacaatgat ttctgcacct ttcgctttcc
    25441 acggaatatt tttagcctct ttttcggcgt aaaccgcgat acttttccca ttaacgataa
    25501 gtgaatcttc cgtaaaatca acgctccagg ggaacggtcc gtagtttgaa tcatgtttga
    25561 gcaggtaggc gagaattttt ggagaagtga gatcattaat agcgacaacg tctatgttgc
    25621 ttttgacttc aagtaatcga cgcaacacca gtcgaccgat acgaccaaaa ccgttaatac
    25681 caactttact catggttttc tcctgtcagg aacgttcgga tgaaaattga tcctttccag
    25741 gcttagacca ggattgtggg atgggcaatc tccattctta cagtgaaacg taacgtactg
    25801 aaaacgggtg aacaatattt aatgaaattt tgagaaaagc ccgttatgtt aacggaaaat
    25861 tatgttgaag caggaaatgt tatggaaaat aaatactcaa ggttacaaat cggcattcac
    25921 tggctggtct ttttgctggt tatcgcagcg tattgcgcaa tggagtttcg tggtttcttc
    25981 ccacgtagcg aacggccgct catcaacatg attcatgttt cctgtggcat ctcaattctc
    26041 gtgctgatgg tggttcgtct tttgttaaga ctgaaatacc caaccccgcc gattatacct
    26101 aagccgaaac cgatgatgac gggactggcg catttgggac atttggtgat ttatctcctg
    26161 tttattgcgc tgccagtgat tggtttggtg atgatgtata accggggcaa cccgtggttt
    26221 gcgtttggtt tgacgatgcc ttacgcttca gaggccaatt tcgaacgggt agatagctta
    26281 aagtcgtggc atgaaacgct ggcgaatctg ggatattttg tcatcggttt gcacgctgcg
    26341 gctgcactgg cacaccacta tttctggaag gacaacacac ttctacgcat gatgccgcgt
    26401 aaacgttcct gaagaatatt taaagaaaac gcctgtacta aaaccgaccc gtggtacagg
    26461 cgaagaatac gggtctacat cggaagcgcc tattatattt atttgtatga caaataaaac
    26521 cataatcctt gcccatacgt ctatctggct tatttttaat caattcaccc gatctttgat
    26581 ctcatcaacg gtatcaaaat aaaccagcgt ataacgttca aatcgaacat aagattcgat
    26641 aaaccatgga ggttatatga aaaaactagc acttattttg tttatgggaa cgcttgtttc
    26701 cttttatgcc gatgccgggc gcaaaccctg ttctggttcg aaagggggga tctcacactg
    26761 tacggcaggc ggcaaatttg tctgtaatga tggttctatt agtgcatcga aaaaaacatg
    26821 cactaactga agtgtaaaag gggtgccatg agaaaatgaa tctgctagtc agatgcgcgg
    26881 ggaaaatccc cgcgcttgcc cttacctgga cgtgcaggcc atgagcgcag caacctcctt
    26941 atcaccgtcc cggaaccgca gttcgtagag cgtttgtcgg gtcaggagtg tgaatgccag
    27001 aatcgtaatg cagataatga gcagacacac caccagaggg ttgcgcttca tagcctttct
    27061 ccttgccgga tggcgggtaa gaggctaaca tctgaattgc ttggttcatt cgttggcctc
    27121 ggttgataga aatatcggtc ggggccttcg tctttctgat tcccggttag cctgaaaaca
    27181 gaaagtctca ggcacccgca ggcatcctat gaggtttcct tagggacgaa aataatcact
    27241 tcacgaaatt gcgtgctgtt ttccagaatt tttcgtcatt cgggttagcc agtttagcca
    27301 ttcgttactc tcttcattcc aatagcatta attttctctg caataattgt tgtaaaaatg
    27361 tgacgcaaag aggtttttgg tcataagtaa ttaccgtcaa gtgccgatga ctttctatca
    27421 ggagtaaacc tggacgagag acaacggtaa tgaatacaac tccctcacag cgattaggtt
    27481 ttttgcatca catcaggctg gttccgttat ttgcctgcat tcttggcggt atcttagttc
    27541 tattcgcatt aagttctgct ctggctggct atttcctctg gcaggccgat cgcgatcagc
    27601 gtgatgttac tgcggagatt gagattcgga ccgggttagc gaacagttca gattttttgc
    27661 gttcagcccg gatcaatatg attcaggccg gggctgcgag tcgtattgcg gaaatggaag
    27721 caatgaagcg aaatattgcg caagccgaat cggagattaa acagtcgcag caagggtatc
    27781 gtgcttatca gaatcggccg gtgaaaacac ctgctgatga agccctcgac actgaattaa
    27841 atcaacgctt tcaggcttat atcacgggta tgcaacctat gttgaaatat gccaaaaatg
    27901 gcatgtttga agcgattatc aatcatgaaa gtgagcagat ccgaccgctg gataatgctt
    27961 ataccgatat tttgaacaaa gccgttaaga tacgtagcac cagagccaac caactggcgg
    28021 aactggccca tcagcgcacc cgcctgggtg ggatgtttat gattggcgcg tttgtgcttg
    28081 ccctggtcat gacgctgata acatttatgg tgctacgtcg gatcgtcatt cgtccactgc
    28141 aacatgccgc actacggatt gaaaaaatcg ccagtggcga tctgacgatg aatgatgaac
    28201 cggcgggtcg taatgaaatc ggtcgcttaa gtcgtcattt acagcaaatg cagcattcac
    28261 tggggatgac agtagggact gttcgacagg gcgcggaaga gatttatcgt ggcaccagcg
    28321 aaatttccgc tggcaatgcg gacctttcat ctcgcaccga agaacaagcg gcggctatcg
    28381 aacaaactgc cgccagcatg gagcaactca ctgcgacggt gaaacagaat gcggataacg
    28441 cgcatcatgc cagcaaactg gcgcaagagg cttctattaa agccagcgat ggcgggcaga
    28501 cggtttccgg ggtagtaaaa acgatgggcg ctatctccac gagttcgaag aaaatttctg
    28561 agatcaccgc cgtcatcaac agtattgctt tccagacgaa tattctggca ctgaatgctg
    28621 ccgttgaagc tgcgcgagcg ggtgagcaag ggcgtggatt tgccgttgtc gccagcgaag
    28681 tacggacact cgcaagccgc agcgctcagg cggcgaaaga gattgaaggc ttgatcagtg
    28741 aatcagtcag gttaattgac ctggggtcgg atgaggtggc aacggcaggg aaaaccatga
    28801 gcactattgt tgatgccgtc gcgagtgtca cacatatcat gcaggaaatc gccgccgcct
    28861 cggatgaaca aagtagaggc ataacgcagg ttagccaggc gatttctgaa atggataagg
    28921 tgacgcaaca gaatgcttct ctggtagaag aggcctcagc agcggcggtg tcccttgaag
    28981 aacaggcggc acgattaact gaggcggtgg acgtattccg tctgaacaaa cattctgtgt
    29041 cggcagaacc tcgcggagcg ggtgaacaag ttagtttcgc tacggtgtga aaatgttcaa
    29101 ggagggatcg acagatccct tcacctttca gaacggcatt gattttcgaa tagcgttaat
    29161 catcaactgg caaccagaag agaacgtcgc atctacgcgg gtcagtattc caatcggttc
    29221 gcctgcacca tgtcccggaa caggcagggc caccagcgtg gcatgacgca ggtcgtcttt
    29281 taccgcgcca gaagggacaa accacacgta gtcgtattca accgtaagtt gacgagatag
    29341 cgaagcagac agcgtttcga tacaacccga aggaatttta cagccctggc tctgcactaa
    29401 tgcatctgaa tgctggcgtg gcgcagtgcc ttctggcgat acaacgaccg gccattccag
    29461 cacccggctt agcgttacgt tctcctgaag tagcgggtga ttagggcgga caaccagctt
    29521 cagcgattca agaaacagca gttcgtaatt aagcccggtc atcagttcag gatctgacat
    29581 ccgaccaatg ccgatatcga tttccccggt ttttaaaccc gccagaatca tagggttact
    29641 cattgtcgca acttgcaagg tcgtctcttt ttgttgctga tgaaactgac ctataaccga
    29701 aggtaatatc cccagtgccg cagtaggtag tgcaccaacc ctgacgacat cattattaag
    29761 accttcttta cgatgaagcg actgtccggc agtgttgatg gcgtcaagaa ctctgactgc
    29821 atgcgttaaa aattgttcgc cgggtaaggt aagttgcgcc ccctgacgac cacgctcaaa
    29881 caagcgagcg ccagtcagct gctccagttc attcaatgtc ttagagagcg caggttgact
    29941 caaattaagg gtttcagccg cgcgccccaa agttccttgt tgtgcgacag ctacgaatgt
    30001 atgaaggtgg cgcaaacgga tgcgctgact aaacagacta tttttttcca taagcgatgt
    30061 taaaaacgaa gcggtgtcgc tgacaagtga agttgtttga ttatgataac ttgattgcaa
    30121 aatattatta acaattaaag caattatgtt acagcaaaat ggataatatt gatgttttcg
    30181 cggcgagatc acagtttgta aattcttccc gccagagtga atgcggttac ctacactcca
    30241 gattactgac cactggaggc agacactatg gcgaacagca tcacggcgga tgagattcgg
    30301 gaacagtttt cgcaggcaat gtcagccatg taccagcaag aagttccgca atatggcacg
    30361 ctgctggaac tggtagctga tgtgaatctg gctgtgctgg aaaacaatcc tcaactgcac
    30421 gaaaaaatgg taaatgcaga cgagctggcg cgactgaatg ttgaacgtca tggggcgatt
    30481 cgtgttggga ctgcacaaga gcttgctact cttcggcgga tgtttgccat tatggggatg
    30541 tacccggtga gctattacga tctctcgcag gcgggggtgc cggtacattc gacagcattt
    30601 cggcccattg atgatccttc tctggcgcgt aatcccttcc gcgtttttac ctcgttactc
    30661 cgccttgagc ttatcgagaa cgaaattttg cgccagaaag cggcggagat tctgcgtcag
    30721 cgcgatatct tcaccccacg ttgtcgacaa ctgttagagg aatatgagca gcagggcggt
    30781 tttaacgaaa cacaggcaca ggagtttgtg caggaagccc tggaaacgtt tcgctggcac
    30841 cagtcagcaa cggtagatga agaaacctat cgcgccttgc acaacgaaca tcggttgatt
    30901 gctgatgtgg tctgttttcc tggatgccat atcaaccacc tgacgccacg tacgctggat
    30961 attgaccggg tgcagtcgat gatgcctgaa tgcggaattg aacccaaaat tctcatcgag
    31021 gggccgccgc gccgcgaggt accgatttta ctacgccaga ccagctttaa agcactggaa
    31081 gagacggtgt tgtttgcggg gcagaaacag ggcacgcata ccgcgcgctt tggtgaaatt
    31141 gagcagcgtg gcgtggcatt aacgccgaaa gggcgacaac tgtatgatga tcttctgcgt
    31201 aacgctggaa ccgggcagga taatctcact caccaaatgc atttacagga aaccttccgc
    31261 acttttcctg acagtgagtt tttaatgcgt cagcaagggt tggcatggtt ccggtaccgt
    31321 ctgacgcctt cgggtgaggc gcatcgtcag gcgattcatc ctggagacga tccacagccc
    31381 ttaattgaac gtggttgggt aatggcgcaa cccatcacct atgaagattt cttgcccgtt
    31441 agcgcggcgg ggatcttcca gtcaaatctg ggtaatgaaa cgcaggcacg cagccacggt
    31501 aatgccagtc gcgaagcatt tgagcaggcg ttgggttgtc cggttttgga tgagttccag
    31561 ctttatcagg aagcggaaga acgcagtaaa cgtcgctgtg gtttgcttta aattctgacc
    31621 atccgccttt gcaaaaattt gcctgatttt acaaacgaat caggctcatc ccatcgacat
    31681 aaaaaaaatg ccgatttatg catattctct cagttcaaca attggattat taataaatat
    31741 tgtctagagt gagcggtcat aaataagcac tttcttgccg ctgaaaacga ccagcgcggg
    31801 accattcaca acaccagaag gactcacttt caggtatgga tcgtagacga tttattaaag
    31861 gttcaatggc tatggccgcc gtgtgcggta ccagcggcat tgcttctctt ttttctcagg
    31921 cggcattcgc ggcagattct gatattgccg acggacaaac ccagcgtttt gacttctcca
    31981 ttctacagtc aatggcgcat gacttagcgc aaacagcgtg gcgtggtgcg cctcgtccgt
    32041 tacctgacac gctggcgaca atgacgccgc aggcttataa cagtattcaa tacgacgccg
    32101 agaaatcgct ctggcataac gttgagaacc gtcaactgga cgctcagttc ttccatatgg
    32161 gaatgggatt ccgtcgccgc gttcgtatgt tttctgtaga tccagcaaca catctggcgc
    32221 gtgaaattca ctttcgcccg gagttgttca aatacaacga tgcaggtgtt gataccaaac
    32281 aattagaagg gcaaagcgat ctcggctttg ccggttttcg cgtgtttaaa gcccccgaac
    32341 tggcgcgccg tgatgtagta tcatttctcg gcgcgagtta tttccgcgcc gttgatgata
    32401 catatcaata cggtttgtcg gcccgcggcc tggcgatcga cacttacacc gacagtaaag
    32461 aagagttccc cgactttacc gccttctggt ttgatacggt aaaaccgggg gcaactacct
    32521 ttaccgttta tgcgttgctc gatagtgcca gcattactgg tgcctataag ttcactatcc
    32581 attgtgagaa aagtcaggtg attatggatg tggaaaatca cctgtatgcg cgcaaagaca
    32641 ttaaacagct gggcattgcg ccgatgacca gtatgttcag ctgcggtact aatgaacgtc
    32701 ggatgtgcga caccattcat ccgcaaattc atgactctga tcgtctgtcc atgtggcggg
    32761 gcaacggcga gtggatttgc cgtccgctga ataatccgca aaaattgcag ttcaatgctt
    32821 acaccgacaa caacccgaaa gggtttggtt tattgcaact ggatcgtgat ttctcccatt
    32881 atcaggacat tatgggctgg tataacaaac gcccaagtct gtgggtggaa ccgcgtaaca
    32941 agtggggtaa gggcaccatc ggcctgatgg aaatcccaac aacgggcgaa acgctggata
    33001 acattgtctg cttctggcag ccagaaaaag ctgtaaaggc gggtgatgag tttgcattcc
    33061 agtatcgtct gtactggagt gcgcaaccgc ctgttcattg cccattagcg cgcgttatgg
    33121 cgacgcgtac cggcatgggc ggtttcccgg aaggttgggc accaggtgaa cactatcccg
    33181 aaaaatgggc gcgtcgtttt gccgtcgatt tcgttggtgg tgatctgaaa gctgccgcac
    33241 caaaaggcat tgagccggtg attacgcttt ccagtgggga agcgaagcaa atcgaaattc
    33301 tctatattga acccattgat ggttatcgta ttcagtttga ctggtatccg acttcggact
    33361 ccactgatcc ggtcgatatg cggatgtatc tgcgttgtca gggcgacgct atcagtgaaa
    33421 catggctgta tcagtatttc ccgccagcgc cggataaacg tcagtatgtt gacgaccgcg
    33481 tgatgagtta atcgtttttt cttcggcacc ttcttcggga ggtgccgtct ggttaaacac
    33541 gatcccgctc gcatttttcc ctaagttaaa tgagtaatct gatggtgtgt atttcagata
    33601 caccttgtca gccactaaca gggagtgcgt atgtttccag aatatcgaga tttaatatcc
    33661 cgtctgaaaa acgaaaatcc tcgctttatg tccttgttcg ataaacacaa taaacttgat
    33721 catgaaattg ccagaaagga aggttccgac ggtcgagggt acaatgcgga agtggtccgc
    33781 atgaaaaaac aaaagctaca gttaaaagat gagatgctca aaatcctgca gcaggagagc
    33841 gtcaaagagg tgtaaacttt cctaagccgc cagccaggcg gcttttttaa caactgcatg
    33901 gattgactgg agataagatg actgaaacga taaaagtaag cgaatcactt gaattacatg
    33961 ctgttgcaga aaatcacgtc actcctcttt atcagttaat ctgtaaaaat aaaacctggt
    34021 tacagcagtc gctaaactgg ccgcagttcg ttcagagtga agaggacacg cgaaaaacgg
    34081 tgcagggtaa tgtgatgttg catcaacgcg gctatgccaa aatgttcatg attttcaaag
    34141 aagatgaact tatcggcgtt atctcgttta atcgtattga accactgaat aaaaccgctg
    34201 aaattggcta ctggctggac gaatctcatc aggggcaggg gatcatttct caggcgctgc
    34261 aggcattgat tcatcattac gcccagtctg gtgaacttag acgcttcgtg atcaaatgtc
    34321 gggtggacaa tccgcaaagc aaccaggtcg ctttgcgcaa tggttttatc cttgaaggtt
    34381 gcctgaaaca ggctgagttc ctgaatgatg cctatgatga tgtgaactta tacgcgcgta
    34441 ttatcgattc ataataaccc tgccagcggc gttcgcgtaa tgcgctcctc gccgttaatg
    34501 acttttggac cacggacatg aaccgtgtcg ccatcaaagg cttcaacgac ggcatggtcg
    34561 gttaactcca catggttttc aattatcacc gcacctgtaa tacggctctc accttgtatt
    34621 acaacgtgct cgtcgagcag aatcggtcca ccacgtacca cggcatttcc gccaatcaag
    34681 acatgatgtt ttaacacaca attaccttcc acaatggcat attccgccac ctgcgaactg
    34741 taatgaatcg ttggaatggc atcttcttct atgccagctt tcacctgcgc gtgaccgtag
    34801 actttagcgc aatcgcatag ccagacgttg ttctcttcat taccttcaat actggcaaaa
    34861 tcaaaaactt cggcgcgatg ttcaataaaa gcataccgga tgacggcatc gccataaatt
    34921 tgtgcctggt ggacaatacg cgaggcgcta acccttgcgc gatcataaat ttgtagcagg
    34981 agttgatggt caggcgttaa gccttgtgct gcgacgatca tagaatgttg attaattaag
    35041 gcgtgaccaa atattctaca ttgcccgcaa accagtgagt catgaatggt gacactgtca
    35101 cttatatatg caccctggct gatttcacta ttatcgatcc agacattatc cgttgcgtaa
    35161 acctctcccc acagtacgct ggtgccggtg atgcgcgtat taccggaaat caccgttccg
    35221 ccgaacgcaa tagcattctg gtcgtaaatc cagcaatttc cttcctgtgc taacaccgtt
    35281 tcgcgatcga tccagccgcc agcggttcct gctatcacat cgttaaaatc gctaatggcg
    35341 ataatctgcc gtaataacac atttttttta gtgccatctt cctgataact aaaggctcgc
    35401 tgttcttcac ttagacgata tttgcgcata gcgttttccc acaggtgact tactataacc
    35461 gtagcaaatt ctgcggctct ggctatgctc gagaaaattc cataaaatgc atttcaaata
    35521 tactttataa attaaacaaa atgagtaaga agatgcagag cgataaagtg ctcaatttgc
    35581 cggcaggcta ctttggtatt gtgttgggga cgatagggat gggatttgcc tggcgctatg
    35641 ccagccaggt ttggcaggtc agccactggt taggggatgg gctggtgatt ctggcgatga
    35701 tcatctgggg attattgact agcgcattta ttgcccgact catacgcttt ccgcatagcg
    35761 tgctggcgga agttcgccat ccagtgctga gcagttttgt gagtttgttt ccggcaacga
    35821 cgatgctggt ggcgattggt tttgttccgt ggtttcgccc actggcggtg tgcctgttca
    35881 gttttggtgt cgtggttcag ttggcttatg ccgcctggca aactgcggga ttatggcgcg
    35941 gatctcaccc tgaagaagct accacgcctg gactgtatct gccgacagtt gccaacaact
    36001 ttatcagcgc aatggcctgt ggtgcgttgg gctacaccga cgccggtctg gtgtttttag
    36061 gcgcaggcgt tttctcatgg ctaagccttg aacctgtgat cttgcagcgt ctgcgcagtt
    36121 cgggagaatt acccacggca ctgcggacat cactcggcat tcagctcgct cctgcgctgg
    36181 tggcctgtag tgcctggctg agcgtcaacg gcggcgaggg tgacacgctg gcgaaaatgc
    36241 ttttcggtta tgggctgctg caactgctgt ttatgctacg tctgatgcca tggtatctct
    36301 cccagccatt taatgcttca ttctggagtt tctcgttcgg cgtatctgca ctggcaacca
    36361 ccggtttgca tctggggagt ggcagcgata atggattttt ccatacgctg gcggtgccgc
    36421 tgtttatctt taccaatttt attattgcaa tactgctcat ccgtactttt gcgcttctga
    36481 tgcagggaaa attgttagtc agaactgagc gcgccgtttt aatgaaagca gaggacaaag
    36541 aatgatcatt cgtgacgaaa actattttac tgataaatat gaattaaccc gcacacactc
    36601 tgaagtactg gaagcggtga aagtggttaa accgggtaaa acgctggatc tgggctgtgg
    36661 caatggtcgt aacagtcttt acctggcagc caatggttat gatgttgacg catgggataa
    36721 aaatgccatg agtatcgcca acgtcgagcg cattaaatct attgaaaatc tggataattt
    36781 acacacccgg gtcgttgatc tgaataacct cacatttgat ggacagtacg attttattct
    36841 ttcgattgtg gtactgatgt tccttgaggc taaaaccatc ccagggctga ttgccaatat
    36901 gcaacgttgc acgaaacctg gtggttacaa cctgattgtg gcggcgatgg ataccgctga
    36961 ttatccatgt accgtcggct tcccgtttgc cttcaaagag ggagaattac gtcgatatta
    37021 cgaagactgg gagatggtga aatacaatga ggacgtcggc gagctgcacc gcaccgacgc
    37081 caacggtaat cgtattaaac tgcgtttcgc cacgatgctg gcacgtaaaa aatgacccgg
    37141 taagaacaaa gcgcgtgaaa attccccacg ctgagatggt ttactgttct tcttttcggt
    37201 aagcatattt tttatcgaag ggatgtgaaa ttaatcacag tagtcgaagt tttttagcag
    37261 cttaacttac tgaaatttaa ctactgatga ttgacttagc ccctttttcg gcattgacga
    37321 tgtcgtctga aaaggggctg aaaaatttat tttcaccaac actttttttg ccacaacacg
    37381 aagcggcgct tttgctataa cttaaaaagt aatataatca tctc
//
LOCUS       DAPGOF010000026        36266 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.026, whole genome shotgun sequence.
ACCESSION   DAPGOF010000026 DAPGOF010000000
VERSION     DAPGOF010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.026"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            297..1670
                     /gene="aroP"
                     /locus_tag="RHV13_001155"
     CDS             297..1670
                     /gene="aroP"
                     /locus_tag="RHV13_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HDV1240709.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1713..2567)
                     /gene="ampE"
                     /locus_tag="RHV13_001156"
     CDS             complement(1713..2567)
                     /gene="ampE"
                     /locus_tag="RHV13_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HDV1240710.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLKAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFRTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2564..3115)
                     /gene="ampD"
                     /locus_tag="RHV13_001157"
     CDS             complement(2564..3115)
                     /gene="ampD"
                     /locus_tag="RHV13_001157"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HDV1240711.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            3203..4096
                     /gene="nadC"
                     /locus_tag="RHV13_001158"
     CDS             3203..4096
                     /gene="nadC"
                     /locus_tag="RHV13_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HDV1240712.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4299..4739
                     /gene="ppdD"
                     /locus_tag="RHV13_001159"
     CDS             4299..4739
                     /gene="ppdD"
                     /locus_tag="RHV13_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HDV1240713.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQNDSALQQACEDVFRFDDAN"
     gene            4749..6134
                     /gene="gspE"
                     /locus_tag="RHV13_001160"
     CDS             4749..6134
                     /gene="gspE"
                     /locus_tag="RHV13_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HDV1240714.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTTETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGF
                     YCRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6124..7326
                     /gene="hofC"
                     /locus_tag="RHV13_001161"
     CDS             6124..7326
                     /gene="hofC"
                     /locus_tag="RHV13_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HDV1240715.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMRWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGIMLTIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMHCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7361..8404)
                     /gene="guaC"
                     /locus_tag="RHV13_001162"
     CDS             complement(7361..8404)
                     /gene="guaC"
                     /locus_tag="RHV13_001162"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HDV1240716.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(8560..8604)
                     /locus_tag="RHV13_001163"
     CDS             complement(8560..8604)
                     /locus_tag="RHV13_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1240717.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8629..9249
                     /gene="coaE"
                     /locus_tag="RHV13_001164"
     CDS             8629..9249
                     /gene="coaE"
                     /locus_tag="RHV13_001164"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDV1240718.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9249..9992
                     /gene="zapD"
                     /locus_tag="RHV13_001165"
     CDS             9249..9992
                     /gene="zapD"
                     /locus_tag="RHV13_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HDV1240719.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            10002..10199
                     /gene="yacG"
                     /locus_tag="RHV13_001166"
     CDS             10002..10199
                     /gene="yacG"
                     /locus_tag="RHV13_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HDV1240720.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10299..10688)
                     /gene="mutT"
                     /locus_tag="RHV13_001167"
     CDS             complement(10299..10688)
                     /gene="mutT"
                     /locus_tag="RHV13_001167"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HDV1240721.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGGPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(10748..13453)
                     /gene="secA"
                     /locus_tag="RHV13_001168"
     CDS             complement(10748..13453)
                     /gene="secA"
                     /locus_tag="RHV13_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDV1240722.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQLQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13515..14027)
                     /gene="secM"
                     /locus_tag="RHV13_001169"
     CDS             complement(13515..14027)
                     /gene="secM"
                     /locus_tag="RHV13_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HDV1240723.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLS"
     gene            complement(14258..15175)
                     /gene="lpxC"
                     /locus_tag="RHV13_001170"
     CDS             complement(14258..15175)
                     /gene="lpxC"
                     /locus_tag="RHV13_001170"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HDV1240724.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15276..16427)
                     /gene="ftsZ"
                     /locus_tag="RHV13_001171"
     CDS             complement(15276..16427)
                     /gene="ftsZ"
                     /locus_tag="RHV13_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDV1240725.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16488..17750)
                     /gene="ftsA"
                     /locus_tag="RHV13_001172"
     CDS             complement(16488..17750)
                     /gene="ftsA"
                     /locus_tag="RHV13_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDV1240726.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17747..18577)
                     /gene="ftsQ"
                     /locus_tag="RHV13_001173"
     CDS             complement(17747..18577)
                     /gene="ftsQ"
                     /locus_tag="RHV13_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HDV1240727.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(18579..19499)
                     /gene="ddlB"
                     /locus_tag="RHV13_001174"
     CDS             complement(18579..19499)
                     /gene="ddlB"
                     /locus_tag="RHV13_001174"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDV1240728.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPAGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMNFSQLVVRILELAD"
     gene            complement(19492..20967)
                     /gene="murC"
                     /locus_tag="RHV13_001175"
     CDS             complement(19492..20967)
                     /gene="murC"
                     /locus_tag="RHV13_001175"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDV1240729.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(21021..22088)
                     /gene="murG"
                     /locus_tag="RHV13_001176"
     CDS             complement(21021..22088)
                     /gene="murG"
                     /locus_tag="RHV13_001176"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDV1240730.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(22085..23329)
                     /gene="ftsW"
                     /locus_tag="RHV13_001177"
     CDS             complement(22085..23329)
                     /gene="ftsW"
                     /locus_tag="RHV13_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HDV1240731.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23329..24645)
                     /gene="murD"
                     /locus_tag="RHV13_001178"
     CDS             complement(23329..24645)
                     /gene="murD"
                     /locus_tag="RHV13_001178"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDV1240732.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24648..25730)
                     /gene="mraY"
                     /locus_tag="RHV13_001179"
     CDS             complement(24648..25730)
                     /gene="mraY"
                     /locus_tag="RHV13_001179"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDV1240733.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25724..27082)
                     /gene="murF"
                     /locus_tag="RHV13_001180"
     CDS             complement(25724..27082)
                     /gene="murF"
                     /locus_tag="RHV13_001180"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDV1240734.1"
                     /translation="MISVTLSQLTDILNGELQGADITFDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(27079..28566)
                     /gene="murE"
                     /locus_tag="RHV13_001181"
     CDS             complement(27079..28566)
                     /gene="murE"
                     /locus_tag="RHV13_001181"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HDV1240735.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     IDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLSLGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28553..30319)
                     /gene="ftsI"
                     /locus_tag="RHV13_001182"
     CDS             complement(28553..30319)
                     /gene="ftsI"
                     /locus_tag="RHV13_001182"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HDV1240736.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30335..30700)
                     /gene="ftsL"
                     /locus_tag="RHV13_001183"
     CDS             complement(30335..30700)
                     /gene="ftsL"
                     /locus_tag="RHV13_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDV1240737.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30697..31638)
                     /gene="rsmH"
                     /locus_tag="RHV13_001184"
     CDS             complement(30697..31638)
                     /gene="rsmH"
                     /locus_tag="RHV13_001184"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDV1240738.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31640..32098)
                     /gene="mraZ"
                     /locus_tag="RHV13_001185"
     CDS             complement(31640..32098)
                     /gene="mraZ"
                     /locus_tag="RHV13_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDV1240739.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(32700..33704)
                     /gene="cra"
                     /locus_tag="RHV13_001186"
     CDS             complement(32700..33704)
                     /gene="cra"
                     /locus_tag="RHV13_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HDV1240740.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(33786..33872)
                     /locus_tag="RHV13_001187"
     CDS             complement(33786..33872)
                     /locus_tag="RHV13_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HDV1240741.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(33884..34375)
                     /gene="ilvN"
                     /locus_tag="RHV13_001188"
     CDS             complement(33884..34375)
                     /gene="ilvN"
                     /locus_tag="RHV13_001188"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HDV1240742.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(34378..36102)
                     /gene="ilvI"
                     /locus_tag="RHV13_001189"
     CDS             complement(34378..36102)
                     /gene="ilvI"
                     /locus_tag="RHV13_001189"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HDV1240743.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
BASE COUNT         8663 a        10152 c         8921 g         8530 t
ORIGIN      
        1 attcacttac caattttgtg gtttttaatt caattaaaac gaatttaaat tcattttgca
       61 tattgagagg ggttgaggct gagctttaca aacggtttct ttttaagcaa ctcatcttca
      121 accatgcata aagcgggtgc attcgctgcc gcataccatt attcttgatc tgacggaagt
      181 ctttttgtaa caattcaaac ttctttgatg taaacaaatt aatacaacaa acggaattgc
      241 aaacttacac acgcatcact gcgtagatca aaaaaacaat caccgcacga ggtttcatga
      301 tggaaggtca acagcacggc gagcagctaa agcgcggcct taaaaaccgc catattcagc
      361 ttatcgcgct gggtggcgcg atagggacag ggctattcct gggtagcgcc tccgtaatac
      421 agtccgcagg gccagggatt atcctgggat acgccattgc tggttttatc gcctttctga
      481 tcatgcgtca gctgggtgaa atggtggtcg aagaacctgt cgcaggctcc tttagccact
      541 ttgcttataa atactggggc agttttgccg gtttcgcctc tggctggaac tactgggtac
      601 tgtacgtttt agttgccatg gcagagctga ctgccgtggg taaatacatt cagttctggt
      661 atccggaaat cccaacctgg gtttctgccg ccgtgttctt tgtggtgatc aacgccatca
      721 acctgacaaa cgtaaaagtg tttggtgaga tggagttctg gtttgccatt atcaaagtta
      781 tcgcagtggt agcgatgatc atcttcggcg gctggctgct attcagtggc aacggcggcc
      841 cgcaggcgac cgttagcaac ctgtgggatc agggcggttt cctgccgcac ggcttcaccg
      901 ggctggtgat gatgatggcg attatcatgt tctcgttcgg tggtctggaa ctggtgggga
      961 tcaccgcagc agaagctgat aacccggagc aaagtatccc gaaagcgact aaccaggtta
     1021 tctaccgcat cctgattttc tatattggtt cgttagccgt tctgctctca ctgatgccgt
     1081 ggacccgcgt taccgccgat accagtccgt ttgtgctgat cttccacgag ttaggcgata
     1141 cctttgtggc gaatgcgctg aacatcgtgg tactgactgc ggcgctctcc gtgtacaaca
     1201 gctgcgtata ttgcaacagc cgtatgctgt ttggtctggc acaacagggt aacgcgccaa
     1261 aagcgctggc gtctgtcgat aaacgcggcg taccggttaa taccattctg gtgtctgcac
     1321 tggttacggc attgtgcgta ctgattaact atcttgctcc ggaatccgca tttggcctgt
     1381 taatggcgct ggtggtatct gcactggtga tcaactgggc gatgattagc ctggcgcata
     1441 tgaaattccg tcgcgccaag caggaacaag gcgtggtaac tcgcttccct gctctgcttt
     1501 atccgctggg taactggatc tgcctgctgt ttatggcggc ggtactggtg attatgctga
     1561 tgaccccagg aatggcgatt tcggtatacc tgatcccggt atggctgatc gtgttaggta
     1621 tcggctatct gtttaaagag aaaaccgcca aagccgtaaa agcgcattaa tctctctacg
     1681 ccctcacccg tacagggtga gggcaataat ctttacacca acgccccgta aatcgtcagt
     1741 agcgcaatca ccaccacgac cacgaatgag gttttcttcg ccattgaaac cgctgccttc
     1801 ggcgtctcca ccttatcgac atgcggttca cgcgccagag agaactgcgc cagacgcgtt
     1861 aatacctgat attgcgaagt acggaaatca cccagcgaag caaaccaggc cggtaacgct
     1921 ttctcaccat gaccgatcaa ggcatatacc acacccgcaa gacgaactgg cacccaatcc
     1981 agtacatgaa gcacggcatc aatgccggac tgtaaacgat gatgcggcgt ctggtatcgc
     2041 gccagccagt attgccatgc acgcaaaaac gcatacccca tcagcgtaac gggtccccag
     2101 gttcccccca caatcagcca gaacagcggt gcaagataaa aacgaaagtt aatccacagc
     2161 aatgcatttt gcagctcacg caaatactca cgttcgtcgc agcctgccgg gacgccgtga
     2221 atcatggtga gttcgccagc catcgtggca cgggcatggc tatcattacg tgaagcagct
     2281 ttcagataag catgataatg aagacgaact ttacctgcgc caatacacag caaaccaatc
     2341 agcagccaca ccagcagcgt gggaacgttg aacaatactc cctgcaatgc gcgtaacagt
     2401 aaaaaagtca cgcccatcgc aataatggtc atgcctaacg tgcgcccgag agaaaaatgt
     2461 ttcacccgcc gaaagaacgc ttcaagacga tgatcaagct gccagtgctc gcccaactta
     2521 aacaggcgct cgaaaattaa caccagtaag gttgtaaata gcgtcatgtt gtctccttgc
     2581 tgaccaacgc acgaaaccgt gcccaatcaa atgcaggacc gggatcggtt ttccgatccg
     2641 gcgcaatatc acaatggccc gtcatgttat tagcgatatc cggatagcaa tcaatcagtg
     2701 cccgcgtaac cgccgcaagc tgttgatact gcgcatcggt atacgccagc gtatcggtgc
     2761 cttcaagctc aatcccaata gaaaaatcat tgcagcgttc gcgcccctga tactgagaga
     2821 ctcccgcatg ccatgcacgt ttatcgaaag gaacatactg gactatttca ccatcacggc
     2881 gaatcaaaca gtgagcggag acgcgcaaat gggcgatctc agcaaagaaa ggatgtgcct
     2941 gcggatcaat agttccagtg aataatgcgt cgatccacgg accgccaaac tcgcctggcg
     3001 gcaggctaat attgtgcacc accagcaggg tgggtgtttc gtcatccggg cggcaatcgt
     3061 aatgtggtga gggaacgcgg cgcgcgccaa ccagccaccc ctgttctaac aacatgcagg
     3121 atctccttat atgtggtgct aatacccggt tcagagtagc atgtttctac cttatgattc
     3181 gttagctatc tggagtttta acatgccgcc tcgccgctat aaccctgaca cccgacgtga
     3241 cgagctgctg gaacgcatta atctcgatat ccccggcgcg gtggcccagg cgctgcggga
     3301 agatttaggc ggaacagtcg atgccaacaa tgacattacg gcaaaacttt taccggaaaa
     3361 ctctcgctct catgccacgg tgatcacccg cgagaatggc gtattttgcg gcaaacgctg
     3421 ggttgaagag gtgtttattc aactggcagg cgacgatgtc accataatct ggcatgtgga
     3481 tgacggcgat gtcatcaacg ccaatcaacc cttgttcgaa cttgaaggcc catcccgcgt
     3541 gctgttaacg ggcgaacgca ctgcgcttaa ttttgtgcaa accctttcag gagttgccag
     3601 taaggtacgc cactatgtcg aattactgga aggcaccaac acgcagttgt tggatacgcg
     3661 caaaacctta cccggcctgc gttcagctct gaaatacgcg gtactttgcg gcggcggagc
     3721 gaatcaccgt ctagggcttt ctgatgcctt cctgatcaaa gaaaaccata ttattgcctc
     3781 tggctcagtg cgccaggcgg tcgaaaaagc gtcctggctg catccggatg cgccagtaga
     3841 agtcgaagta gagaatctgg aagaacttga tgaggccctg aaagccggag ccgatatcat
     3901 catgctggat aacttcgaaa cagaacagat gcgcgaagcc gtcaaacgca ccaacggcaa
     3961 ggcgctactg gaagtgtctg gcaacgtcac tgacaaaaca ctgcgcgaat ttgccgaaac
     4021 gggcgtggac tttatctccg tcggtgcgct aactaaacac gtacaggcac tcgacctttc
     4081 aatgcgtttt cgctaattgc tgacagattt atccgctcca tagtgggcgg ataatcaccc
     4141 cttccccaac tttcttcgta acgcctcgca aatttcttgt ctttcgctgc aacggaataa
     4201 aaaacctcgg aagctgcttt cccgtttctt tttttgcccc tcgcttgtgc tgatggcgtt
     4261 tgccaaagta gcgccaaccc aatcaaggag cgaaacagat ggacaagcaa cgcggtttta
     4321 cacttatcga actgatggtg gttattggca tcattgccat tttaagcgcc attggtattc
     4381 ccgcttatca aaactacctg cgcaaagccg cactcaccga catgctacaa acctttgtgc
     4441 cttaccgtac cgccgtagag ttgtgcgcgc tggaacatgg tggattagat acctgcgacg
     4501 gtggcagcaa tggcattccc tcgcctacca ccacccgcta tgtttcagcc atgagtgtgg
     4561 caaaaggcgt ggtgtcgctg accgggcaag aaagtctcaa tgggctaagc gtcgtcatga
     4621 cgccgggttg ggataacgca aacggcgtca ccggctggac gcgcaactgc aatattcaaa
     4681 atgacagcgc attgcagcaa gcctgcgaag atgtcttccg ctttgatgac gccaactaag
     4741 gagcggcaat gaatattcca cagctcacgg ccctgtgtct gcgttatcag ggagtcttgc
     4801 tggatgccag cgaagaagtg gttcatgttg cggtggtcga tgccccctca catgagttgc
     4861 tggacgcatt gcatttcgct accaccaaac gtattgagat cacctgctgg acgcgccaac
     4921 aaatggaagg tcacgccagt cgcacacaac agacattgcc cgtagctgtt caggagaagc
     4981 atcagcccaa agcagagttg ctgactcgaa cgttacaatc tgcgctggaa caacgcgcgt
     5041 ctgatattca tatcgaacca gcggacaatg cctaccgcat ccgcttgcgt atcgacggcg
     5101 tattgcatcc tttaccggat gtttcaccgg atgccggagt cgcgttaacc gccagattaa
     5161 aagtgctggg aaacctggat attgcggaac atcgcctgcc gcaggacggg caattcactg
     5221 tcgaactggc aggaaacgcc gtctcatttc gtattgcgac cttaccatgt cggggtggtg
     5281 aaaaggtggt attaaggttg ttacagcagg tgagccaggc actggatgtt aacacgctgg
     5341 gaatgcagcc gttacaactg gcggactttg ctcatgcctt gcaacaacca cagggactgg
     5401 tgctggtaac tggccctacc ggcagcggca aaacggtcac gctttatagt gccctgcaaa
     5461 cgctgaatac cgctgacatt aatatttgta gcgtcgaaga tccggttgag atccccatag
     5521 ccggactaaa ccagacgcaa atccatccgc gtgccggact cacctttcag ggcgttttgc
     5581 gtgcgttatt gcgccaggat cctgacgtca tcatgatcgg agagatccgc gatggcgaaa
     5641 cggcagaaat tgccattaaa gccgcgcaaa ccggtcacct ggtgttgtct accctacaca
     5701 ccaattccac cactgaaacg ctggtacgtt tacagcaaat gggagtcgcc cgctggatgc
     5761 tctcatcagc gcttacgctg gtaatagccc agcgtctggt acgtaaactt tgcccacatt
     5821 gtcgccgcca gcaaggggag cccatccaca ttccagacaa tgtatggcca tcgccgctgc
     5881 cccactggca ggcacccggt tgtgtacatt gctaccacgg tttttattgt cgtacggcct
     5941 tatttgaagt tctgcccata acgccggtca ttcgtcagct tatttccgct aataccgacg
     6001 ttgaatcgct ggaaacgcac gcacgacagg cgggtatgcg aacgcttttt gaaaacggct
     6061 gcctggccgt ggagcaaggc ttaaccacct ttgaagagtt aatccgcgta ctggggatgc
     6121 cgcatggcga gtaagcaact ctggcgctgg catggcataa ccggcgacgg caatgcgcaa
     6181 gatgggatgc gatgggcaga gagccgtgct ttgctgctca tggcactaca gcaacagatg
     6241 gttactccac taagcctgaa gcgaattgct atcaattctg cgcagtggcg aggagataaa
     6301 agcgcggaag tcattcatca actggcgacg ctacttaaag ccgggttaac gctttctgaa
     6361 gggctggcac tgctggcgga acagcatccc agtaagcaat ggcaagcgtt gctgcaatct
     6421 ctggcacacg atctcgaaca gggcattgcc ttttccaatg ccttattacc ctggtcagag
     6481 gtatttccgc cgctctatca ggcgatgatc cgcacgggtg aactgaccgg taagctggat
     6541 gaatgctgct ttgaactggc gcgtcagcaa aaagcccagc gtcagttgac cgacaaagtg
     6601 aaatcagcgt tgcgttatcc catcatcatt ttagcgatgg caatcatggt ggttgtggca
     6661 atgctgcatt ttgttctgcc ggagtttgcc gctatatata agaccttcaa caccccacta
     6721 ccggcactaa cgcaggggat catgacgctg gcagacttta gtggcgaatg gagctggctg
     6781 ctggtgttgt tcggcattat gctgacgata gccaataagt tgctgatgcg ccgcccgacc
     6841 tggcttatcg cgcggcagaa attgctgtta cgcatcccga ttatgggttc actgatgcgg
     6901 ggacaaaaac tcacgcagat ctttacgatt ctggcgctga ctcaaagtgc aggcattact
     6961 tttttacagg gcgtagagag cgtcagagaa acaatgcact gcccgtactg ggtgcaactt
     7021 ctgacacaaa tccagcacga tatcagtaac ggtcatccca tctggctggc gctaaaaaat
     7081 gccggtgagt ttagcccgct ctgtttgcaa ttagtgagaa cgggagaggc ctccggctcg
     7141 ctggatctca tgttagacaa cctcgcccat catcatcggg ataacacaat ggcgctggcg
     7201 gataacctag cagccttact ggaaccggcg ttgctgatca taacgggagg aattatcggt
     7261 acgctggtgg tggcgatgta tctgccaatt ttccatttag gcgatgcgat gagtgggatg
     7321 ggataacgct ggcgtgttgc tccacaccag cgttggaaga ttacaggttg ttgaagatgc
     7381 ggttttcttg ttccagcaca cggataaatg tggtgcgctt ggtcagttct ttcaggcgtg
     7441 aagccccaac gtatgtacaa gctgaacgca ggccgcccaa aatatcacgc gcggtatttt
     7501 caaccgggcc tcgcagcggc agcttaacgg ttttaccttc tgctgcgcga tattcagcaa
     7561 cgccgccaac gtgacgtttc atcgcagact cggagctcat gccgtagaac agcataaatt
     7621 tctcgccgtt ctcctcaacg atgcgaccgc cgctctcttc gtggcccgcc agcatgccgc
     7681 caagcatgac gaaatcggca ccgccgccaa aagctttcgc gacatcgccc ggcgtggtgc
     7741 agccaccatc gctgacgatc attccgccca gaccgtgcgc agcatcggca cattcgatta
     7801 ccgcagaaag ttgcggataa ccgacgcctg ttttgacacg agttgtacaa acagaacctg
     7861 ggccaatgcc aactttaacg atatcggcac cggagaggat aagctcctca cacatttcac
     7921 cagtcactac gttgccagca caaatggttt tggtcggcca cgcttcacgc gctttcgcaa
     7981 cgaactgcac gaagtgttcg gaataaccat tcgccacatc aatacaaacg aagtttaatg
     8041 ccgggttcag gtcgagaatc tgtttagttt tttcgaaatc cgcatcagac gtaccggtag
     8101 aaaccatcac atgtttcagc acatcagcgg aagaattgtt gataaacgct tgccactctt
     8161 cgacagaata gtgtttatgc acagcagtca aaatatcaaa agaagccagc gcagaggcca
     8221 tagaaaatgt gcctacggtg tccatatttg cggcgataat cggcacgccg gaccagctct
     8281 gacctgaatg tttgaaggtg aattgacgtt ccagttcaac atcggaacgg cttttaagag
     8341 tggagcgttt agggcggatg agaacgtctt taaaacctaa cttcagatct tcttcaatac
     8401 gcatgtgcgg attcctgggg ttaatggcta taagggtaag agcgcaactc cagtgacgct
     8461 atcatacgca ctaatcaatg cggcgcaaga ctgcgaaatt gtcttttttt acgctacaat
     8521 cccataaatt tacctggtaa atagtgagcg aacgtttcat caatttcgct ttcgtgcgct
     8581 catttttaaa gttttgataa tcatgatgaa ttcccgggaa tataaattat gaggtatata
     8641 gttgccttaa cgggaggcat tggcagtggc aagagtaccg ttgccaatgc gtttgctgat
     8701 ctcggaatta acgtcattga tgccgatatt attgcgcgtc aggtggttga accaggtgca
     8761 cctgcgctac atgccattgc tgatcacttt ggcgctaaca tgattgctgc tgatggaaca
     8821 ttgcagcgcc gggccttgcg cgagcggatc ttcgccaacc cggaagagaa aaactggctt
     8881 aacgccctgc tgcatccgct gattcagcaa gagacgcaac accagatcca gcaagctact
     8941 tccccctatg tactgtgggt tgtgccattg ctggtagaaa actcactgta taaaaaagcg
     9001 aatcgagtgc tggtggtgga tgtcagccca gaaacgcaac ttaagcgcac catgcagcgc
     9061 gatgatgtaa ctcgcgagca tgtcgaacaa atccttgctg ctcaggcaac gcgcgaagcc
     9121 cgccttgccg tggcagatga cgtcattgat aataacggcg caccggatgc tatcgcatcg
     9181 gatgttgccc gcctgcacgc acactatttg cagcttgcgt cgcagtttgt ctcacaggaa
     9241 aaaccgtaat gcagacccag gtcctttttg aacatccact aaatgaaaaa atgcgtacat
     9301 ggctgcgcat tgagtttttg attcagcaac tcaccgttaa tttacccatc gttgaccacg
     9361 ctggcgcgct gcatttcttc cgtaatgtca gtgaattact ggatgttttc gagcgcggcg
     9421 aagtccgcac tgagctgttg aaagaacttg accggcagca acgtaaactc cagacctgga
     9481 ttggcgtgcc tggcgtggac cagagccgta ttgaagcatt aattcagcag ttaaaagcgg
     9541 cggggagcgt attaatctcc gcgccgcgta tcgggcaatt tctacgtgaa gatcgtttga
     9601 ttgctctggt gcgtcagcga ctgagcatcc caggcggctg ttgcagcttt gatttaccaa
     9661 cattgcacat ttggctgcat ctaccccagg cgcagcgcga cagccaggta gaaacctgga
     9721 ttgccagcct gaatccgctc acccaggcac ttaccatggt gctggattta attcgccagt
     9781 cggccccctt ccgtaaacaa accagcctga atggttttta tcaggataac ggtggcgatg
     9841 ccgacttgct gcgcctgaat ctgtcgctcg attcacagct ttatccgcaa atttccggac
     9901 ataagagccg ttttgccatt cgttttatgc cgctggacac tgaaaacgga caggtaccgg
     9961 aacgtctgga tttcgaactg gcctgttgct aaggagtaaa aatgtcagaa actattacgg
    10021 tgaattgccc aacctgcggg aaaacggtgg tgtggggtga aatcagcccg tttcggccat
    10081 tttgctccaa acgttgtcag ttgatcgacc tcggagaatg ggctgctgaa gaaaaacgaa
    10141 tccccagcag tggcgatctt tccgaaagcg atgactggag cgaagaacca aagcagtgac
    10201 atttgtctgg tgccgcacgt aggcctgata agacgcggac agcgtcgcat caggcatctt
    10261 gtgcaaatgt cggatgcggc gtgaacgcct tatccgacct acagacgctt aagcttcgca
    10321 attaccggtt cattggctgg cggaaaatca tcggcattaa gaccgaccag cgacatccac
    10381 tcaccgggtt gcccttcttt accccacggc cccccttccc aactttcgac cagccagaac
    10441 cacagagtta tatgcctgtc cgggaattca tattccagtt tttcaaatag cgaaaaatgt
    10501 tgtggggtaa tcccgacctc ttcctgaagt tcacgcacca ccgcctgttc cggcgtttcg
    10561 cccatttcaa ttttaccgcc gggaaactcc agtttattcg ccatgtgcgc atctgctgcg
    10621 cgacgcgtta taaagatttc attgttctcg ttgcgaataa tacctaccgc aatttgcagc
    10681 tttttcattg tcttaaacct ataaaaaagg cgcagaatcc tgcgcctttt gcttcaacag
    10741 ttagctttta ttgcaggcgg ccatgacact gcttgtattt tttaccagaa ccgcacgggc
    10801 aaggatcatt acgtcctact ttgcgttcac cggtttgcgc cgccagtgca gctgcggctg
    10861 cagagtcgtc atcctgatgg ctaagctgct gcaattgcgc taaacgctcg gcttccatac
    10921 gacgctgttg ttccagctcc tcaacctctt caggcatacg tacctggact ttgcttaacg
    10981 tgctgataac ttcatatttt aacgactcca gcatcgctgc aaacatggag aacgattcac
    11041 gtttgtattc ctgcttcgga tctttctgtg cgtagccacg caggtggata ccctgacgca
    11101 gatagtccat cgccgccagg tgctctttcc acagggagtc aagcgtttgc agcatgacgc
    11161 ctttctcgaa gtgacgcatc atctcagcac caaccacttc ttctttacgc tgatacactt
    11221 cgatggactg cgccagaatg cgctcacgca gcgtctcttc atgcagttct ggttctttat
    11281 ccagccactc ggcaattggt aaatcgaggt cgaaatcgtt cttcagacgt tcctgcagcc
    11341 ccggaatatc ccacatttct tccagcgact gtggcggaat gtaggcatca atggtggctt
    11401 tgaacacatc ttcacgaatg ctgttgatgg tttcgctcac atcgctgaca tccagcagtt
    11461 cgttacgctg ggagtaaatg gcgcgacgct gatcgttagc cacgtcatca tattccagca
    11521 gttgcttacg aatgtcgaag ttacgacttt caactttacg ctgggcgttg gcaatcgctt
    11581 tagtcaccca cgggtgttca atggcttcgc ctggcttcat acccagttta cgcatcatgc
    11641 cggatactcg gtcggaagca aaaatacgca tcagcgcatc ttccatcgac aggtagaaac
    11701 gggaagaacc agcatccccc tgacgaccag aacgaccgcg caactggtta tcgatacgac
    11761 gggattcgtg acgctcggta ccgatgatat gcaggccacc tgctgccagt accgcatcgt
    11821 gacgtacctg ccagtcggct ttaatttttt caatttgctc tgcggtcgga ttttccagcg
    11881 cggcaacttc tgcctgccag ctaccaccga gcacaatatc tgtaccacga cccgccatgt
    11941 tggtcgcgat agtcaccgca gccggataac ctgcctgagc aacaatcgcc gcttcgttgg
    12001 cgtggaattt ggcgttcagg acattgtgct taataccggc tttggtcagt tcgtttgaca
    12061 ccagctccga tttttcgatg gagatagtac ccaccagcac cggctggcct ttcgcagtac
    12121 gttctttgat atcttcaatg atcgcctgaa ttttttccgc ttcagtcatg tagaccaggt
    12181 ccggcagatc tttacgaatc attggacggt tggtcggaac aacgacggta tccagtttgt
    12241 agatggagct aaattcgaaa gcttcggtat cagcagtacc ggtcatcccc gccagttttt
    12301 catacagacg gaagtagttc tggaaggtga tcgaagccag cgtttggttt tcgttctgga
    12361 tctgcacacc ttctttcgct tccacagcct ggtgcagacc atcggaccag cgacggccct
    12421 gcatggtacg accggtgtgt tcgtcaacga tgataacttc accatcttta acgatgtagt
    12481 cgacgtcacg ggtaaacagc gcatgagcgc gcagcgccgc cgttacgtgg tgcatcagca
    12541 tgatgttggc cggagagtac agagactccc cttcatccat gatgccctct ttcaccagca
    12601 gttcttcaat cagcaccaga ccacgttcgg tcaggttcac ctggcgagat ttttcgtcca
    12661 ccgagaagtg gccttcgccc tggaaggttt cggagtcttc tttttcctga cggatcaggt
    12721 gcggaataat tttattcacg cgtttataca tttccgagct gtcttctgcc gggccggaaa
    12781 tgatcagcgg tgtacgcgct tcatcgatca ggatggagtc cacttcgtcc accagcgcat
    12841 agtgcagttt acgctgtaca cgttcttcag ggctgaacgc catattgtcg cgcaggtagt
    12901 caaagccgta ttcgttgttc gtaccgtaag tgatgtcagc tgcgtaagct tcgcgctttg
    12961 ccggtgctgg catgcccggc aggttgatac cgacagtcag gccaaggaat tcaaacagcg
    13021 gacggttgtt ttcggcgtca cgttgcgcca ggtagtcgtt gacggtaact acatgcacgc
    13081 ctttaccggt tagtgcgttc aggtaagcag gcagcgttgc ggtcagggtt ttaccttcac
    13141 cggtacgcat ttcggcgatg cagcgttcgt taagaaccat accgccgagt aactgaacgt
    13201 cgaagtgacg cataccaaag acgcgcttac ttgcctcacg taccacggcg aaagcttccg
    13261 ggatcagatt ttccagcact tcgccttttt ccagacgtgc acgaaactct gcggttttcc
    13321 ctttcagttc ttcgtcggag agtttttcca tctccggttc catggcattg atgatgttga
    13381 ccactttgcg catccggcgc agagtgcgat cgttacgact accgaaaact ttagttaata
    13441 atttgattag cataataaaa tctcaaacgc cccgcgttgc ggagttaata aaatgaagta
    13501 aaggtttatt gttgttagct gaggcgttga gggccagcac ggatgccttg cgcctggctt
    13561 atccagacgg gcgtgctgaa ttttgcttgt ggggtaaaat gcgcataatc aatgcgataa
    13621 cccttttcag acggcgtgcc ttcctgggtc agaagcgcgc tgagcgtatc cagtaatgca
    13681 agatgttgcg cctgaagagg caaagattct tcagcaacgg gcagcgtttg cggtgccatt
    13741 gcgaaggaaa gatgacggat taccgtgcga atggcatgtt gatgccagta atctacggaa
    13801 tagttcgaat tcgggcggcg cgtgttcgct tccagcaagg ccaattgacc aaagttaact
    13861 ttggctgaag gttcgtggtt gcgggttgtc gcttttgcgg gcgcgtttgg ttcggcggcg
    13921 ttgctgagcg caggcaaacc taaactcgcc gcgaccatcc ctaacaagag atgcggccag
    13981 aagtagcgtt taccaaactg tcgccagcgc gtcagtattc cactcacgtt attgccatcc
    14041 cgatcgtatt caaacgtatt tagcgcacaa atcttatcat taaagcctga agtccacagc
    14101 acgaatgaag ataagttgtg cgtaaaagtg cttaagaaaa cagcgttcgc accgtttttt
    14161 atagctctta taaaattcaa aaaaaaacga ctggttcacc tggccggaga gagtgccaga
    14221 tttgccagtc gaattttata cgacagtata aatgtcgtta tgccagtacg gctgaaggcg
    14281 ctttgaaggc caacggcagt tctgcgtcgt cctggaaggt cacatattcc caggcttcct
    14341 gtttcgccag gacagcctgc agcagtttgt tattcagtgc atgaccggat ttataagcgg
    14401 taaatgcacc aataatattg tgaccacaca tgaacaagtc accgatcgca tcaagcattt
    14461 tgtgacgcac aaattcgtct tcaaaacgca ggccgtcttc gttcagtacg cgataatcgt
    14521 caacaacgat ggcacaatcg aagctgccgc ccaggcacaa accacgggac tgcagatatt
    14581 cgatatcacg catgaaaccg aacgtacgcg cacggctgat ctggcgcata aacgcatcag
    14641 cggagaagtt catcgcatag cgctggttgc tggaatcaat agccggatgg ttaaaatcga
    14701 tggtgaaatc cagcgaaaaa ccattgtacg gcttaaattc agcccactta tcgccatctt
    14761 cgacacgaac agtctctttg atgcgaacaa atttcttggc gcagttcaac tcgtcgatac
    14821 cggcgtcaag cagcaggtat acaaacggag cggcgctgcc gtccatgatc gggatttccg
    14881 gcgcgttaac ttcgataaca atgttatcga tgcccaggcc cgcgagagca gcattgaggt
    14941 gctctacggt tgaaatccgt acatcatgct cgttgaccag acacgtacag agcatggtat
    15001 cacgcacaga tttggcatcg gccgggaaat ctaccggtgg attcaagtcg gtgcgacgat
    15061 agatgacccc ggtgttggcc ggcgcagggc gtaacgtcag ggtgactttc ttgccggtat
    15121 gtaaaccgac acccgtcgcc tgaacgatac gtttaagtgt cctttgtttg atcatcgtat
    15181 tatctcgcca aattacctat ccaaccgaag tgtactatac attcggcggg ccagtttagc
    15241 acaaagagcc tcgaaaccca aattccagtc aattcttaat cagcttgctt acgcaggaat
    15301 gctgggatat ccagataatc cggctctttc gcagtttgcg gcgcattgtc attcacgact
    15361 ttagcaaccg gcttctgctc ctgggtcagc ggagccatcc catgctgctg gtagcgatcc
    15421 atcactggct gctgaacctg cttattggtc accagagtga tttcaggacg tttgtccatg
    15481 ccgatacctg tcgcaacaac ggttacgcgc agctcgtcat tcatatccgg gtcaagagaa
    15541 gtaccgataa ccacagtcgc gttgtcggaa gcaaatgcac ggatggtgtt acctaccgtt
    15601 tcgaactcat ccagacgcag gtcgaagccc gctgtgatgt taaccagcac gccgcgtgcg
    15661 ccagacaggt cgatatcttc cagcagcgga gaagagatag ccatttcagc agcttcttcc
    15721 gcacggtctt caccgctcgc cacgccagaa cccatcattg cgtagcccat ttcagacatt
    15781 acggtgcgta cgtctgcaaa gtccacgttc atcaaacccg gacgagtaat cagttcggcg
    15841 ataccttgca ctgcgccttt gagtacatcg ttcgctgcgc caaacgcatc cagcagggag
    15901 ataccgcggc ccagaacttt cagcagtttg tcgttcggga tagtgatcag agagtccaca
    15961 tgcttggaca gttcagtgat cccctgctcg gcgaatgcca tacgcttctt gccttcaaag
    16021 ttgaagggct tagtgacgac agcaacggtc aggataccca aatcttttgc cacttcagcg
    16081 acgactggtg ctgcacctgt accggtacca ccacccatac ccgcagcaat aaagaccatg
    16141 tctgcacctt ccagcgccgc acgcagtgca tcgcgatcct catcagccgc attgcggcca
    16201 acttctggat tagcgccagc tcccagtcct ttggtgatac cgctaccgat ttgaatcgtc
    16261 tgtccaaccg ctgttttacg cagcgcttgt gcatcggtat ttaccgcgaa gaattcaaca
    16321 ccttcaatgc gctcgcgcac catgtgttca acagcattac cgccgccgcc gccgacgccg
    16381 atgactttaa tcaccgcgtc attggtaagt tccattggtt caaacatagt ttctctccga
    16441 tttgtgcctg tcgcctgaga ccgtaatcat tgtcggcctc ataaaaatta aaactctttt
    16501 cgcagccaac tattgagtcg cttgatccac gagccaactg atgctgtaac acgtttttct
    16561 acttcagctt caccgttaag atgtgactct ttcccatagt gaagcaatcc caccgccgtc
    16621 gaataatacg gctcctgagc ataatccgtt aaaccggtaa tgttcagcgg cgcgccgata
    16681 cgcacttgcg tatgaaacac gcgctgagca caggctgcaa gaccttcgat ctgcgctgcg
    16741 ccaccggtta atacaatgcc tgccgccagg tgatgtttaa ccccttgttg gcgaagcttt
    16801 tcctgcaact gcaatatctc ttcgttgacc aggttgagca gctcggtata gcgcggctcg
    16861 atcacctctg ccagggtctg acgttgcaga ctccgcggcg gacgaccgcc tacgctcggc
    16921 acttccacgc tttcatcttt tccaacgatg gaacccagcg cacaaccgtg gcgaacttta
    16981 atcgcttcgg cgtcgcttgg cggcgtgcca aaggcgtaag cgatatcact ggtcacgaca
    17041 ttgccagcat aaggaattac cttagtgtgg cgcaatgccc caccggtata aacggcgata
    17101 tccattgtac caccaccgat atcgacgacg cagacaccca gttcacgttc atcttccgtc
    17161 aataccgaat aacttgatgc cagtccggca aatatcagtt ggtcaacttt cagcccacaa
    17221 cgttcaaccg ctttgacgat gtttttcgcc atatcgttgt gacatgtgat caggtgcact
    17281 tttgcctgca tccgcacgcc cgaaagtcct accggattct tgatcccttc ctgatagtca
    17341 atcgcatact cttgcgggat cacatgcagc acacgatgct catcgcgcac acgcaccgat
    17401 ttcgcggtat ggacgacgtt ttccacatct tcttgcgtta cttcttcttc agaaataggc
    17461 accataccaa tttcattctg gcagctgata tgcttaccag aaagcgccag atataccgaa
    17521 gagatctgac aatctgccat caattctgcc tggtcaatgg cgcgctgtac gcacttgacc
    17581 acggattcga ggtcgttcac cccgccttta tccataccac gcgacgggca gctgcccacg
    17641 ccaatgatat tgaccatacc gtcgggcaga acttccccta ctaaagcggc aaccttcgcg
    17701 gtaccaatct ccagtcctac taccagtttt ctgtccgtcg ccttgatcat tgttgttctg
    17761 cctgtgcctg attttgttgc tgagtagatt cctctggcgg caagggcgcc cagcctactg
    17821 ccgctccaga gtcataacgc aaatcaacgt agctaatccg tttgccatcg gtttgcgcct
    17881 gctgctgtaa aaccggataa agttctacaa agcgagccaa acgtttcatc gtatcgcccc
    17941 ggccaagatt gagcttaata tcgttattca gcgtcaactg ccaggaacgc cgcgcggtca
    18001 tcgccgcttc cttcagagta aatctgtcct ttgccagcat ctgccccatt tcgcgatagc
    18061 cctgcaacac ttcattggcg ctgccttccg ggccatacag cattggaagc acctgcttgc
    18121 tggtgcgatc tggcggcacg ctgaaggtgt ttccttccgc gtctaccatg tgttgatcat
    18181 tccaccgcgc aatcggcaca tattcaacca gatgaatctt caattcatca ggccactgct
    18241 ttctgacgct cacctgctta atccacggca ggcgttgttc tatttgcgtc tggatgatgt
    18301 tgacatcctg ggtcataaag gtacctggct cacccaatgc caggatcgac tgccggatat
    18361 cgtcattacg cgtgtaatgg cgttcaccgg tcaacaccag ctttgagagc ggcaggcgtt
    18421 gcgcatcttc catccagccc aacacgaccc agccgctcac caacactgtc gttaaaacgg
    18481 tcagcaggaa aaggatcccc gccagacgcg ttccattatt gcggcgagaa gaaacctctt
    18541 cttcgctgtt tcgcgtgttc agagcagcct gcgacatatt agtccgccag ttccagaatt
    18601 cgtactacca actgcgagaa gttcatacct gcctgacgtg ccgccatcgg caccaggctg
    18661 tggctggtca tacccggtga ggtattggct tccagcagat aaaactgtcc atcgctgtcc
    18721 agcataacgt caatacgtcc ccatcctttg caacctaacg tcgtccatgc tttcagcact
    18781 aatgcctgca aattggcctc ttgtgacgct tccagaccag cggggcagaa atactgtgtc
    18841 tcatcagaga gatacttcgc ctcataatca tagaaggttc cggccggttg aatacgtatt
    18901 gacggtaaaa tttcttcacc gagtatcgca accgtgaact ccggcccact tagccatttt
    18961 tcaatcaata cttcttcatc gtgctgaaat gccaatctta atgcatcttg tagagcattt
    19021 tctgctacta cttttgacat tcccacactg gaaccttcgc ggctcggctt aacgataacc
    19081 ggcaaaccca gagcagaaat ttctgctaac tgcttatcac tcaggccttt ttcaaactct
    19141 gcgcgggtta acgctaccca cggcgcgacc ggtaaaccgg caccttgcca cagaagtttg
    19201 ctgcgtagtt tatccattga aagcgcagat gccatcactc cgctgccggt ataaggcaag
    19261 cccatcagct cgagcatgcc ctgcagcgta ccatcttcac cgccgcgacc gtgtagcgcg
    19321 ataaacactt tctgaaagcc catcgacttc agttgcgtca catcgacttc tttcgggtcg
    19381 acaggatacg catcaatacc gccttcacgc agcccggcta acaccgctgc gccagaattc
    19441 agagaaactt cccgctcagc ggaagtcccg cccaacagga ccgcgatttt atcagtcatg
    19501 ttgttcttcc tccggagttt gcggcttcag tttgatttca gctaaagaac gggcaatttt
    19561 tccgatatta ccagccccct gaacgagaat caggtcgtta ccggttaata ccggtgccag
    19621 catctcggct acctgcgccg gatccggcac cagaatggga tctattttcc cacgtccacg
    19681 aattgtgcga cacagcgaac ggctgtccgc acccggaatt ggcgcttcgc cagccggata
    19741 cacttccagc atcaacaggg tatcaacctg cgtcagcaca ttggcgaaat catcatacag
    19801 gtcgcgtgta cgggtaaaac ggtgcggctg aaacagcatt accaggtttt tatccggcca
    19861 gcctgcgcgc gccgctttaa tggtggcgtc cacttccgtc gggtggtggc cgtagtcatc
    19921 gaccagcatt gccgtaccgc ttttaccatt cactggctcc agcgggaatt caccgaggaa
    19981 atcaaaacgg cgaccagtcc cctggaaact ctccagcgcc cgcaaaatag cctcgtcatc
    20041 aatgccctct tccgtagcaa ccgcaaccgc agctgcggcg ttcagcgcgt tgtgacgacc
    20101 tggtgcattc agggtgacgc gcatcggctc tttgtcctgg cgcagcagcg taaagtgccc
    20161 ctgcgggcca atctgctgat aatcttctac acgcacgtcg gcatcttcgc tgaagccgta
    20221 agttgtggtc tgacgtccca ctcgcggtaa caattcgcgg atcaccggat catcaacaca
    20281 catcaccgca cgaccgtaaa acggcaggtt gtgcagaaaa ttaataaaag tctgttttaa
    20341 attctcaaag tcgccctggt aggtatccat gtggtcggct tcgatattgg tgacaatcgc
    20401 caccatcggt tgcagatgca ggaacgatgc atcactctca tctgcttcgg caatcaggta
    20461 ccgaccatgc cccaaacgcg catgaacccc cgccgctttt accagcccgc cgttaacgaa
    20521 ggttgggtcg agccccgctt ctgcgtagat gctggaaacc atcgcggtgg ttgtcgtttt
    20581 gccgtgcgtt cctgcaatgg cgatgccatg acgaaaacgc attaactcag ccagcatttc
    20641 ggcacgacgg atcaccggaa tacgcgcttc atgagctgcg acaatttccg ggttatcggc
    20701 agaaatggcg ctggaaacaa cgaccacgct ggcatcacgt acgttttccg ggcgatggtt
    20761 gaaataaatc gtcgctccca gattcattaa ctgctgcgtg accggatttg gcgctaaatc
    20821 ggaaccactg atctgataac cttcattggc cagaacttcg gcaataccgc ccataccggc
    20881 accaccgatg ccgacaaaat gtatgtgccg aacgcgacgc atttcgggca cgatggaacg
    20941 cagttttgcc aattgttgtg tattcattct ttacgccatt aacttcttaa attcatacga
    21001 tgcaaaaggc atcgctacaa ttacgcccgg gcagcccggc tcacttcatt tgccactcgc
    21061 tcggtggcat ccggaatgga tgcagcgcgg gcgcgttctg ccatggttaa taaggtttct
    21121 cgcgaccacc cggccagggt gttggcgaca gcatccacgc taagctgtgg ctgctcgata
    21181 attttggctg cgcccgcttt ttccagcggt agcgcattcc agtattgctg gcggtcttta
    21241 tgttgaaacg gcacaaacaa cgccggtagt cctgccgcgg cgatttcact caccgttaaa
    21301 gcaccggagc ggcaaacgac gacatccgcc cacgcatacg ccgccgccat atcatcaata
    21361 aattcagtca ctttatgctg cggctgcccc gcttcggcat acgcctgttc aacggattgt
    21421 tgcgaacctt tgccgctctg atgccagata gtgactgaat cacccagttt cgcagcaacc
    21481 tgcggcattg tctggttaag aatgcgtgcg ccctgggaac cacccactac cagcacacga
    21541 accggacctt cacgtccagc caaacgttgc tgcggcaacg gtagcgccaa cacatcggta
    21601 cgcaccgggt tacccaccac ttccgcattg gggaaagcac ctggaaacgc ctgcatcact
    21661 ttggtggcaa tcttcgccag ccatttattg gttaagcccg caataccgtt ttgttcatga
    21721 agtacaaccg gaatgcctaa cgaccacgcg gccagaccac caggacctga tacgtagccg
    21781 cccataccga gcaccacgtc tggtttgtac gctttcataa tcgcccgtgc ctgacgccag
    21841 gcgttgaaga tacgcagcgg cgcagctatc agtgctttta taccttttcc acgcagacca
    21901 gagatacgaa tgaaatcaat ttcgatgcca tgttttggca ctaagtccgc ttccatacgg
    21961 tcggcagtcc ccagccagcg aacttgccaa ccctgagcca ttagatggtg cgcaaccgcc
    22021 agtcccggga atacatgtcc accggttccg cctgccatca ccattaatcg ctttccttga
    22081 ccactcatcg tgaacctcgt acaaacgcct gcgctttctc cagacgcgtt tcataatcaa
    22141 tacgcaacag catcatgatg gctgtcgaca taatcagtaa gctcgaacca ccgtaactga
    22201 tcagcggcaa tgtcagacct ttggtcggta acatccccgc cgccgcgcct acgttaacca
    22261 gcgcctggaa gctaaaccag atgccaatag aacaggcgag aaaaccggaa aaacggtggt
    22321 caatttctaa cgctttgcgg ccaatcgaca tcgcgcgaaa agcgacgaag aataccatta
    22381 aaagtgccag caccacaccg acatacccca gttcttcgcc gataatggcg aaaataaagt
    22441 cagtgtgcgc ttccggcaga tactccagtt tttgtaccga gttacctaaa ccttgtcccc
    22501 aaagttcgcc gcgaccaaac gccatcagcg attgcgttaa ctgatagccg ctgccaaagg
    22561 gatcttccca cgggttccag aatgcggtaa cacggcggat acggtacggt tcggcgagta
    22621 tcagcaacac aaccgctgaa atgcccatac cgataatggc aatgaactgc cacaatttcg
    22681 ctcccgccag gaacaacatc gccagcgtag tcacaaacaa caccaccacc gtaccaaggt
    22741 ctggctgtgc cagcagtaac actgccaaca ccagaatcac gcccatcggt ttcaggaagc
    22801 cgcgcaggtt attacgtact tcgtcgcctt tacgcaccag atagttggcg atataacaaa
    22861 acagcgacag ttttgtcagc tccgcaggct ggatacgcag caaaccgaga tcgatccaac
    22921 gcgatgcccc tttaaccgag ctacccacta ccagcacgat catcagcagg atgatagatc
    22981 cgagcagcat cgtggcactg tagcgttgcc agaactccat cggcagacgc agcgtaatga
    23041 tcgccagaat gaatgccagg atcagataga cgccatcacg cttcgcgaag aagaacggat
    23101 cgttggttaa gcgttgccct atgggcattg acgccgaggt caccatgata aagccaatcg
    23161 ccgcaaggcc gaaggtcagc cacagtaagg tgcgatcgta catgatcagg ctgtcggtat
    23221 ctttttcccg cgagcccatc acccagcctt ttagcgccgt ggagatccag accaggatac
    23281 tgaatcctgg caggcgcggc attttcaggc gagggagaga taaacgcatc aacctaactc
    23341 cttcgccaga cgggcaaact cattgcctcg ttgttcaaag ttcttgaact gatcaaggct
    23401 ggcacaggcc ggggagagca gaaccatatc gcccggctga acacgcggag ccagcaagcg
    23461 catcgcctgt tccatagttt cggtttgttc tgccacttcc gggcgtagcg ccgccagctg
    23521 cgcgccgtca cgaccgaaac aatacagacg tacgttatcg ccattcaggt aacgcgctag
    23581 tgggctaaag tccgccgatt taccatcgcc gcccagcaac aaatgcagcg tgccgtctac
    23641 gtgcaggcca ttcagcgccg cttccgtact gccgacgttg gtcgcttttg aatcgttaat
    23701 ccaacgtacg ccgttatgct ccagcacaac ttcaaagcga tgcggcagac cagtgaatgt
    23761 ggttaatgct ttcaggctgc tggcgcgcgg caaaccagct gcgtctgcca gtgccagtgc
    23821 tgccagcgca ttggtgtagt tatgctgccc ggaaagtttc atttctttca cgttcagcac
    23881 tttctcgcct ttcacccgca gccaggtttc gccctgctga tgattcaggt gatagtcacc
    23941 catgttgacg ccaaagctga cgcagcgttc atccgcaccg cgaatcggca ttgttaaggc
    24001 atcatcagca ttaaccacgc aaactttcgc gttttcgtaa atgcgcagtt ttgctgcacg
    24061 atactgttgt aaaccaaacg gatagcgatc catatgatct tcagtcacgt tcagaatggt
    24121 cgctgctacc gcctgtaagc tggaggtggt ttccagctgg aagctcgaca gttccagcac
    24181 gtacagttca cactcatcat ccagtagcat caacgcaggc aggccaatat tgccacccac
    24241 accaacgtta acccccgccg ctttcgccat ttcacccact agcgtggtga ccgtgctttt
    24301 gccgttagaa ccggtaatcg ccacaatcgg tgcttgtgct tcgcgacaga acagctcgat
    24361 atcgccaacg atttcgattc cggcatcagc ggcagcgctt aaggatggat gcgccagtgc
    24421 aataccggga ctggcgacaa tcagatctgc cgccatcagc cattcatcat tcagactgcc
    24481 cgtgtggcgt tctacggctt cgggtaattt atccaggcca ggcggtgtca tacgcgtatc
    24541 cataacgcgc ggcgtcacac cgcgagcgag gaaaaagtcc acgcaggaaa gcccggtgag
    24601 gcccaggccg ataatgacga catttttacc ctgataatca gccatgatta acgtaccttc
    24661 agcgttgcca gaccaatcag gaccagcatc agcgaaataa tccagaaacg cacaatgacg
    24721 cgcggttccg gccagccttt cagttcatag tggtgatgaa tcggtgccat gcggaaaata
    24781 cgttgtccgc gcagtttaaa ggagccgacc tgcaggatga cagaaagcgt ttctaccacg
    24841 aacacgcccc ccataatcac cagcaggaat tcctgacgta gcagtacggc gataatgcct
    24901 aacgcaccac ctaacgccag cgaacctaca tcgcccataa agacctgcgc cggataggtg
    24961 ttaaaccaca ggaagcccag tcctgccccg actatcgccg tgcagacaat gaccagttcc
    25021 cctgcgtgtc gcagatacgg tatatgcaag tagctggcaa agttcatatt gccggtcgcc
    25081 cacgccacca gcgcaaaacc accggcgaca aatacggtcg gcataattgc caggccgtcg
    25141 agaccatcgg tcaggtttac cgcgttgcca gtacccacaa tgacgaagta agccagcaga
    25201 atgtagaaca gccccagctg cggcatcaca tctttaaaga atgggaccac cagctgcgtt
    25261 gcgggcgtgt ctttgccggc aaggtacagg gcgaaggcga cacccagcgc aatgaccgac
    25321 atccagaaat acttccaacg agcgatcaac cctttggtgt ctttacgcac cactttgcga
    25381 taatcatcaa caaagccaat aacaccgtaa cctaccagca ccaccaacac gcaccagacg
    25441 tacggattgg acgggtaagc ccacagcagt acggagatca caatcgccgt caggatcata
    25501 atcccgccca tggtcggcgt accgcgcttg ctgaagtgtg attcaggacc gtcgttacgc
    25561 accacctgac caaaggaaag tttttgcaaa tgagcaatca tacgcgggcc catccacaat
    25621 gagatgaaca gcgcggtcag caggctgacg atggcgcgaa acgtcagata ggaaaagacg
    25681 ttaaagccgg aataatattt gaccaaatgt tcggccagcc aaactaacat gtcccattct
    25741 cctgtaaagc gcgtactacc tcttccatgg cggcactacg tgaaccctta actaaaatcg
    25801 taattacctg ttgctcagca atcagtgatt taagacgcgt aattaacgca gttttatcag
    25861 caaaatgttc gccaacgccg ctggcggtgc tgatagcatg gctttgttta cccacgctta
    25921 acacgcggtc aataccagca gcttttgccg cctcgcccac ctgtacatgg caggcttcgc
    25981 tttcagcgcc cagttccgcc atatcgccca ccaccagcac gcggtagccc ggcatttcag
    26041 ccagtacctg aacagcggcg gtcattgaac cgacattggc gttgtaggag tcgtcgagca
    26101 gcaactggtt ttctgccagt tggatgggga acagacggcc tggaacagct ttcagatttg
    26161 ccagccccgc tttgatagca tcaagcgttg cgcccacgga catggagagc gcagcggctg
    26221 ccagcgcatt cgcaatattg tgacgccccg gcaacggcag cagaacatcc acgctaccgg
    26281 ttggggtttg tagggtaaat tccgtaccgt gcgaggtcac atggatattg gtggcggtga
    26341 aatcgctgtt ggcggcattg ggtgagaaac gccacacttt gcgtgagcca attacgctct
    26401 gccagttcag ccagtcgttg ttgtcggcgt tcataatggc gataccgttt tccggcaggc
    26461 cgctaaagat ttcacctttc gctttcgcga cacccgcaag cgagccaaaa ccttccagat
    26521 gcgccgctgc caggttgttg accagcgccg cttccgggcg agtcagactc acagtccagg
    26581 caatttcgcc ctgatggttc gcgccaagtt caataactgc gtaatcgtat tccggcgtta
    26641 agcgcaacag cgtcatcggt acaccgatgt cgttgttgag attgcctgcc gtataaagcg
    26701 tgttgccgca ctggcttaaa atcgccgccg tcatctcttt aacggaggtt ttgccggagg
    26761 aacctgtcag agcaaccacg cgcgccggaa cttgctggcg aacccatgca gccagttcac
    26821 caaacgccag acgcgtatcc ttgacgatta actgcggcag gtcgatgtcc agcggacggc
    26881 taaccagtag tgcgcctgcg ccgccagctt tcgcctggtc ggcaaaatca tgagcatcga
    26941 aacgttcgcc tttcagggca acaaacaggc agcccggcgt cagttttcgg gtatcagtgg
    27001 ttacagcatc aaaggtgata tctgcacctt gcagttcacc gttgagaatg tcggtaagtt
    27061 ggctaagggt tacgctaatc atgcaatcac ccccagcaga cgcgccaccg tgacgcgatc
    27121 ggagtagtcc agacgctgat tgccaacaat ctggtaatct tcatggcctt tgcccgcgac
    27181 cagcaccaca tcgttctctt tagcctgcat aacggcgcaa gtcaccgctt cagcacggcc
    27241 ttccatcact ttggcatgtc cggcatctaa cattcccgcc agaatatcgt tgatgatggc
    27301 acgcggttct tcggtacgcg ggttatcgtc cgtcaccacc gccacgtcag caaactcttc
    27361 ggcaattgcg cccatcagtg gacgcttacc tttatcgcga tcgccaccac agccaaagac
    27421 acaccacagc ttgcccgcac agtgcagacg cgccgcctgt aaggcttttt ccagtgcatc
    27481 cggtgtatgc gcgtaatcca ccaccaccgt cggtttgcct ggcgcagtga acacttccat
    27541 acgtccgcaa accggttgca gacgcgcggc ggttttcaac agatcagcca atggatagcc
    27601 gagtgacaac agtgtcgcca gcgcgagcag caggttgctg acgttaaaag cgcccatcag
    27661 atggctttca atttcgccat cgccccaact tgagctaaag cgaatcgtcg caccgctgtc
    27721 gtgatagttc acgtcgatcg cttttaacca gcgtccgtga cagttcggat tgatatgatc
    27781 ttccattgat accgcaaccg cgtccggcag ttttgccagc cagcggcggc ccacttcatc
    27841 gtcggcgtta ataatcgcct gaccgcaatg atgttcagaa taaagcagcc atttcgcggc
    27901 ttcgtagtgt tccatatcac catgataatc aaggtgatcg cggcttaagt tggtaaagac
    27961 cgacgccgca aatttcaatg ccgccacacg gtgctgtacc agcccgtggg aggaaacttc
    28021 cattgcgcaa aacgtcgcgc cctgatccac cagccccgcc agctcgtgct gaacatcgac
    28081 tgccgaaccg gtagtatttt ctgtcgggat cactttcccc agcagaccgt taccaacggt
    28141 acccattacc gcgctggttt cgccaagcag ttggctccac tgcgccagca gctgggtagt
    28201 cgtggttttg ccgttggtgc ccgttacgcc tacgagacgt aaattgtcag agggttcatg
    28261 gtaaaagcgg cccgccagtg cagataaacg ctcattgagc tggctgagat agatgaccgg
    28321 tacgccgtgc atttcacgga tttcaccgtc ggtcgcctca tctttcgcct ctgcaataat
    28381 ggcagccaca ccttgcgcta tcgcctgcgg gatatatcga cgcccgtccg cctgatgacc
    28441 tactacagct acaaagagat cgcccgccgc agccacacgg ctgtcgagtg tcatctctcg
    28501 cagtgctcgc gaaggtgcgt ctggcaccca cggagcaaga aggtcgcgca aattacgatc
    28561 tgccacctgt cccctcgcct tgattaatca caaattcatt tttatcgccc gttgtcagcg
    28621 catccggctc gatgttcatg gtacgcaata cgccgcccat gatggcacca aagaccggcg
    28681 ctgaaacggc gccgccgtag tatttacccg cctgcggatc gttgataaca acaaccagcg
    28741 cgaagcgcgg ctgactcgca ggcgcaacgc ctgcggtata agcaatatat ttattgatgt
    28801 agcgaccgtc cggcccgacc tttttcgcgg taccggtttt aatggcgata cgatagcctt
    28861 taatcgccgc cttcacgccg ccgccgcccg gtagcgccac gctttccatc atatgcacca
    28921 cggtgcggac aatggattcc gggaagacac gttcaccggg aaccgggggg tcaactttgg
    28981 taatcgacag tgggcgataa atgccgtagc tgccgatagt tgcgtagact cgcgctaact
    29041 gtaacggtgt taccattagc ccgtagccga aagagaaggt ggccctctct atgtcagacc
    29101 accgttgttt ttgaggatat aagccactgc gttctccgac caaccccaaa ttggtcgctt
    29161 ttcccagtcc aaaacgtgag taagtatcta ctaacgctga ggacggcatc gctaacgcca
    29221 gcttggaaac accgacgtta ctcgacttct gtaatacccc ggtcagggtt aattcgctgt
    29281 agcgtgccac gtctttgatt tcgtggccgt taattcgata aggaatggta ttgagtaccg
    29341 agttttcccg caccacgcca cgttgcaacg cggtcatcac caccatcggt ttaaccgttg
    29401 agccaggttc aaacacgtcg gtgatggtac ggttacgcat cgcctctttc ggcgtgccgc
    29461 tcagattgtt agggttgtat gacgggctgt tagccatcgc cagcacttca ccggtgttga
    29521 catccaccag cacggcgcta ccagattcag ccttgttaaa ggccaccgca ttgttcagtt
    29581 cgcgataaac cagcgcctgc aggcgttcat caatactcag cgccaggttg tgcgcagcct
    29641 ggctgtcagt agaagaaata tcttcaatta cgcgaccata ccggtcttta cgcacaatgc
    29701 gctcacccgg ctgcccggta agccatttat cgaaactctt ctcgacgccc tcaatccctt
    29761 gactatcgac gttagtaaag ccaatgaggt gagcagtcac ttcgccggat ggataataac
    29821 ggcgagattc ttcacgcaga tgaatccctg gcagtttcag ttttttgatg tagtccgcca
    29881 tgtcagggtt tacctgacgc gccagataaa taaagcgccc tttcgggttg gcgttaatgc
    29941 gggctgaaag ctgatccagc ggaatattga gcgcgttagc cagcgccttc cagcggtcac
    30001 cgacgctgat accgccagcg tcatgcactt ctttcggatc agcccaaatc gcttttaccg
    30061 gcacgctcac cgctaacggg cgaccagaac ggtcagtaat catgccgcgg gaggtggaaa
    30121 cttgctgaac gcgaagagaa cgcatgtcgc cctctttcac cagcatatcc ggggagataa
    30181 cttgtaacca cgctacgcgt ccgagcagaa aagccagcgc caggagaata cagccgcata
    30241 acaacgcaaa acgccaactg ataaagttgg catgttcttc ctgacgtttt ggtttctgcg
    30301 ttttcgccgc tgctttcatg cgtcgcgttt atccttattt ttgcactacg atattttctt
    30361 gtgacggatc aacatgctgc atttgcagct tttccgtggc aatcctttcc acccggctat
    30421 ggtcgccgag cgcattctct tcaaggatca ggttgcgcca ttcaatgtct aaagcatctc
    30481 gctccagcac cagttgttcg cgctgagcgg tcagtaaacg ggtatggtgc gccgtggtta
    30541 ccacggtcac cgccgtcaaa ataatgcaaa tgaacaggca gagtggcagc ttcccaaatc
    30601 gcaaaagatc gtcaccgata acaccaggca atgcatggcg ctcgtggctt cccatcgatc
    30661 ctttaacttt gcttagagct tctgtcactc tgctgatcat gcgttcgtcc tctctgcaat
    30721 acgcagaact gaactacggg cacgaggatt ctcagccacc tcttcttcgc ccggcattaa
    30781 cttgcctaat gctcgcagct gacggccacc cagttttttg agctgctctt cagtcatcgg
    30841 taaccctgcc ggaacttgcg gaccgcggct gttttcacgc ataaaacgtt tcacaatacg
    30901 gtcttccagc gagtggaagc tgatgatcga aagccgccca cccggggcca gcacgttgag
    30961 cgagcttttt agcgcctgct ctatctcctc cagttcactg tttacccaaa tgcgcaccgc
    31021 ctggaaggta cgggtcgcgg gatgtttgaa tttgtctttc accggcgttg cggcagccac
    31081 gacttccgcc agttctttgg tgcgggtcat cggctgttcg cggttacgct cgacaatggc
    31141 gcgggcaatg cgtttggcaa aacgctcttc accataggtt ttcaataccc aggcgatatc
    31201 ggcttcttct gcggtttgta gccattcagc ggctgactgc ccacgggttg ggtccatacg
    31261 catgtccagc ggaccatcgc gcataaagga aaagccgcgt tcagcatcat caagttgcgg
    31321 tgaagagaca ccaagatcga ggagaatgcc gtcgatcttg ccgataagat cgcgctcggc
    31381 aacgtattcg cccagcgcgg aaaaaggtcc gtggatgatg gagaagcgcg gatcatcaat
    31441 agtcttcgca acggcgatag cctgcgggtc gcgatcgatc gccagcaaac gcccctcttc
    31501 gccaagctgc gagaggatca gacgtgagtg accaccgcga ccaaaagtcc catcaatgta
    31561 gatgccatca ggacggatat tgaggccatt aacggcttca tccagcagca ccgtagtatg
    31621 tttatagttt tccatcattt tatagagaca agtcctgcag tcgctccgat aagtctccgg
    31681 tagccaactg ctctgcgtcg atatcttcct tgacctgttg atgccaggtt gtttcatccc
    31741 acagctcaaa cttgttgaac tgtccaacca gcatcacttc ttttgtcagc ccggcatgtt
    31801 gccgcagtac tggcgcgatt aacaatcgac ctgcgccatc catctgacat tcgctggcat
    31861 gacctaacag taggcgctgc acacggcgct caaccgggtt catgctcgac agacgcgata
    31921 atttttgctc gataatttcc cattcaggca gggggtaaag cagcaggcac gggtgatgaa
    31981 tgtcaatggt gcaaaccatt tgaccggcag cgttctcaag cagctgttcc cgataacggg
    32041 taggcactga taagcgccct ttgctgtcga gattgactaa cgttgctccc cggaacatgc
    32101 cagcctcacc ccttattccc actttgcccc acaatttccc actgaaagga gtttacggag
    32161 cggaggaaaa gcttgtcaag ccagtcacaa ggcataccga ggtaaaaaac ccgatgttta
    32221 cggctaatat cagcgttgtt tattttacgt ccaacgtgcg cagcaaatta acgttatgaa
    32281 tagctgtaag aaaaaatccc tcatcgtcat caccgtttta aaatgactaa ctattttcca
    32341 cagcaaatat aaagtgtcag tttgcgacgc gagcggcatt ttaggacata tcttccccgg
    32401 ttaacagtcc ctgttgcgtc tgtgtagcgc ttgatgccag cgggtttgcg caaaagcaaa
    32461 gaaaagtgct tgttaaatcg gcaattcatc tctgcatgta acaaaataat gcaaaaacgc
    32521 gctgtcgata tcacttaatt attgtgaatt tcagctggtg agttaattaa cgggcgggtt
    32581 taaaagcatt tatcaataat agtcaggaat agtcttattt actttaagga tattgatgtc
    32641 cagtcccgta ctctacgcgc cagagggaaa ttcacctggc gcgtattttt gttcgcggct
    32701 tagctacggc tgagcacacc gcggcgatag agattacgtt taatgcgcgt taaaccaggt
    32761 tttggcttac gcggttcgtc caggcttgcc aggacaatct ccagcacacg ctctgcgaca
    32821 tcgcggtgac gttgagccac tgccagcacc gggcactgta agaagtcgag cagttcatta
    32881 tcgccaaagg tggcaattgc caggtcagaa ggcagtttgc cgtcgcgacg cagcgtgaca
    32941 tccattactc cttgcaacaa cgcaaacgac gttgtgaaca gcgcctgcgg catcggatgc
    33001 gtttccagcc atttttcaaa taactgggca gccgcctccc gctcatagct gttggcatac
    33061 aggaaatgca cttcgcgcgg atcatctttc caggcagtac ggaaaccttg ttcacgcagg
    33121 aagctgacag aaagctccgg cagcgcgcca agataaagca ccgtctcggc gggaaactta
    33181 cgtaactctt ccgccagcat ttcggcatca tcctgatcgg caccaaccac gctggtgaaa
    33241 tgttcacgat cgagggcgcg gtccagcgcg acaatcggga acgggtcgtt agcccagcgt
    33301 tgataaaaag gatgctcagg tggcaacgac gtcgaaacaa taatggcatc aacctgacgc
    33361 tgtaaaaggt gctcaatgca gcgcatttcg ttgtctggct gatcttctga gcaggcaatc
    33421 agcagttgat aaccccgttg ccgcgcctgg cgttcaagat agttagcgat gcgggtatag
    33481 ctggtgttct ccagatcggg gatcacaaga ccaatagaac gtgtgcgtcc agcacgaagc
    33541 ccagctgcca cggcattcgg gtggtaattg tgctcacgca cgacagccat gactttttca
    33601 acggttttgt cgctcacacg gtattgcttc gctttgccgt taataacata gcttgcagtg
    33661 gtccgcgaca ctcccgccag ccgagcgatt tcatccagtt tcacaattgc cccttgcgta
    33721 aaatgtaaaa gccatagcca ttgtactggt atgggttaaa ttcattaaca tctaagcgca
    33781 gaaaattaac tgcggcaact gcttttattc caggttccca ctgatttcgc aaaaaaagcc
    33841 caacgtgaca tgttgggctg caaattgcgc actgagatca ttctcaacgc attattttat
    33901 cgccgcgcga aagtccgacc acaccagagc gagcaacttc cacaattttc gccacatcgc
    33961 gaatcgatgc taaaaatgca tcaagcttat cgctggtgcc tgctaattga acggtataaa
    34021 gcgagggggt gacatcgata atttgcccac ggaatatttc cgtattacgt ttcacttcgt
    34081 cacgcccgta accgctggcc tgaattttca ccagcatgat ttcccgctca acatgcgcgc
    34141 cctgccccaa ctcactcacg cgcaagacat cgaccagctt gtgtaattgc ttttcgatct
    34201 gctcaagtac tttttcatcg cccacggtct ggatggtcat acgcgataat gtcggatcgt
    34261 cggttggcgc aacggtcagg ctttcaatgt tgtagcctcg ctgggaaaaa aggccaatca
    34321 cgcgggataa cgcgcctgat tcattttcga gtaagactga taatatccgg cgcataatca
    34381 ggttctctcc gttttgctta accacatttc atccattccg cccccgcgaa tctgcatcgg
    34441 gtagacgtgc tcactgccat cgacggtaac atcaacaaac accaggcgat tattgcgcac
    34501 ctgttccagc gcctcgctaa gtttgctttc cagctcatgc ggatgagaga tctggatccc
    34561 gacgtgacca taggcttccg ccagacggac gaaatcgggt agtgattgca tataagattg
    34621 tgaatgacgg ccggaataga tcatgtcctg ccactgtttc accatcccca gatagcggtt
    34681 attgagattc accaccagta cgggcaactc gtattgcaac gcggtagaca gttcctgaat
    34741 gttcatctga atactgccgt cgccagtgac gcaaaccacg gtttcttctg gcaacgccat
    34801 tttgacgccc agtgccgcag gtaaaccaaa acccatcgtg ccgaggccac cggaattgat
    34861 ccagcgacgc ggtttgtcga atggataata aagtgcagca aacatctggt gctgcccgac
    34921 atcggacgtc acgtaagcgt ctcccttcgt caaccgccaa agagtctcga tcaccgcctg
    34981 cggtttaatc ttttcactgt gagtgtcata tttcaggcac tgacgagcgc gccactgttc
    35041 aatttgctgc caccagtcgc ggatctcatc cagtggttga tgggcggatt cttgcgacaa
    35101 gagttcaagc atttgttcga ggacctggcg agcatccccc acaatcggga tatccgcagt
    35161 cacggtttta gaaatggaag taggatcaat atcgatatgc agaacagtgg catttgggca
    35221 gtactttgcc agattgttcg tcgttcggtc atcaaatcgt accccgacgg cgaaaatcac
    35281 atccgcgtta tgcatcgtca tattggcttc gtaggtaccg tgcattccca gcatgcccag
    35341 tgcctgacga tgcgttgccg gaaacgcccc cagccccatc aatgaggaaa caacgggcag
    35401 attcaacgcc tccaccgttt ctttcaactg ctgatggcag cccgccgtga ttgccccacc
    35461 gcctacgtag acaaccggtt tttttgccgc taccagcgtt tgcagagcgc gcttaatttg
    35521 ccctttatgt ccggtagtag tgggattgta agaacgcata ctgaccgact ccggccagac
    35581 atagggtaat ttgttcgccg gattaagaat atctttcggt aaatcaacga ctactggtcc
    35641 aggacgacca cttgccgcca gccagaaagc ctttttcagc acctgcggaa tgtcttccgt
    35701 ttgcttaacc agaaaactgt gtttaaccac tggtcgcgaa atccccacca tgtcgcactc
    35761 ctgaaaggca tcgtaaccta tcaacgaggt cgctacctgc ccggaaagga caactaatgg
    35821 aatggaatcc atataagcgg tggcgatgcc ggtaatcgca ttggtcgccc ctggacccga
    35881 tgttaccagc acgacgccga cttccccggt cgcgcgcgcc agaccatcgg ccatatgcac
    35941 tgccgcctgc tcatgacgaa ctaaaacatg atcgatacca cccacggtat gcaatgcatc
    36001 gtaaatatca aggactgcgc ctccgggata accgaatact tgtttaacgc cctgatcgat
    36061 aagcgatcgg acgaccatct cggctccaga caacatctcc atggcctgcc tcactgtttg
    36121 acggaaaaaa tgtgtaaaac acattaaccg tccgatcttg cttaagcaat tggcagccag
    36181 agacataaac gccgggtaat aagaataaac aggaggaaag gtgaaaagaa acgataaaat
    36241 cctccattac gaaggataaa aatgat
//
LOCUS       DAPGOF010000027        35272 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.027, whole genome shotgun sequence.
ACCESSION   DAPGOF010000027 DAPGOF010000000
VERSION     DAPGOF010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.027"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            159..1526
                     /gene="dcuC"
                     /locus_tag="RHV13_001190"
     CDS             159..1526
                     /gene="dcuC"
                     /locus_tag="RHV13_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HDV1240744.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPTKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERTGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKALDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(1570..2067)
                     /gene="sspB"
                     /locus_tag="RHV13_001191"
     CDS             complement(1570..2067)
                     /gene="sspB"
                     /locus_tag="RHV13_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HDV1240745.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDASIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(2073..2711)
                     /gene="sspA"
                     /locus_tag="RHV13_001192"
     CDS             complement(2073..2711)
                     /gene="sspA"
                     /locus_tag="RHV13_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HDV1240746.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(3106..3498)
                     /gene="rpsI"
                     /locus_tag="RHV13_001193"
     CDS             complement(3106..3498)
                     /gene="rpsI"
                     /locus_tag="RHV13_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDV1240747.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3514..3942)
                     /gene="rplM"
                     /locus_tag="RHV13_001194"
     CDS             complement(3514..3942)
                     /gene="rplM"
                     /locus_tag="RHV13_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDV1240748.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(4161..5288)
                     /gene="zapE"
                     /locus_tag="RHV13_001195"
     CDS             complement(4161..5288)
                     /gene="zapE"
                     /locus_tag="RHV13_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HDV1240749.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGGKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5482..5880
                     /locus_tag="RHV13_001196"
     CDS             5482..5880
                     /locus_tag="RHV13_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HDV1240750.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            6034..7401
                     /gene="degQ"
                     /locus_tag="RHV13_001197"
     CDS             6034..7401
                     /gene="degQ"
                     /locus_tag="RHV13_001197"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HDV1240751.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            7491..8558
                     /gene="degS"
                     /locus_tag="RHV13_001198"
     CDS             7491..8558
                     /gene="degS"
                     /locus_tag="RHV13_001198"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HDV1240752.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGSI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(8621..9559)
                     /gene="mdh"
                     /locus_tag="RHV13_001199"
     CDS             complement(8621..9559)
                     /gene="mdh"
                     /locus_tag="RHV13_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HDV1240753.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            9994..10464
                     /gene="argR"
                     /locus_tag="RHV13_001200"
     CDS             9994..10464
                     /gene="argR"
                     /locus_tag="RHV13_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HDV1240754.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            10829..11092
                     /gene="yhcN"
                     /locus_tag="RHV13_001201"
     CDS             10829..11092
                     /gene="yhcN"
                     /locus_tag="RHV13_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HDV1240755.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(11147..11419)
                     /locus_tag="RHV13_001202"
     CDS             complement(11147..11419)
                     /locus_tag="RHV13_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HDV1240756.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQAFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(11510..13477)
                     /gene="aaeB"
                     /locus_tag="RHV13_001203"
     CDS             complement(11510..13477)
                     /gene="aaeB"
                     /locus_tag="RHV13_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HDV1240757.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            complement(13483..14415)
                     /gene="aaeA"
                     /locus_tag="RHV13_001204"
     CDS             complement(13483..14415)
                     /gene="aaeA"
                     /locus_tag="RHV13_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HDV1240758.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            complement(14423..14626)
                     /locus_tag="RHV13_001205"
     CDS             complement(14423..14626)
                     /locus_tag="RHV13_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HDV1240759.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            14809..15738
                     /gene="aaeR"
                     /locus_tag="RHV13_001206"
     CDS             14809..15738
                     /gene="aaeR"
                     /locus_tag="RHV13_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HDV1240760.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            complement(15866..17311)
                     /gene="tldD"
                     /locus_tag="RHV13_001207"
     CDS             complement(15866..17311)
                     /gene="tldD"
                     /locus_tag="RHV13_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HDV1240761.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(17650..21450)
                     /gene="yhdP"
                     /locus_tag="RHV13_001208"
     CDS             complement(17650..21450)
                     /gene="yhdP"
                     /locus_tag="RHV13_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HDV1240762.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRVELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGDV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPAAVNEALSGSVAWDGKVGIELPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTQVEAQGREINATLAMRNNAPWLANIKYLYYNPSMAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDLTISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKTPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            complement(21518..22987)
                     /gene="rng"
                     /locus_tag="RHV13_001209"
     CDS             complement(21518..22987)
                     /gene="rng"
                     /locus_tag="RHV13_001209"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HDV1240763.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            complement(22977..23570)
                     /gene="yhdE"
                     /locus_tag="RHV13_001210"
     CDS             complement(22977..23570)
                     /gene="yhdE"
                     /locus_tag="RHV13_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HDV1240764.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKARAGVAQTAKDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            complement(23579..24067)
                     /gene="mreD"
                     /locus_tag="RHV13_001211"
     CDS             complement(23579..24067)
                     /gene="mreD"
                     /locus_tag="RHV13_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDV1240765.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            complement(24067..25170)
                     /gene="mreC"
                     /locus_tag="RHV13_001212"
     CDS             complement(24067..25170)
                     /gene="mreC"
                     /locus_tag="RHV13_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDV1240766.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            complement(25236..26279)
                     /gene="mreB"
                     /locus_tag="RHV13_001213"
     CDS             complement(25236..26279)
                     /gene="mreB"
                     /locus_tag="RHV13_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HDV1240767.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            complement(26584..28524)
                     /gene="csrD"
                     /locus_tag="RHV13_001214"
     CDS             complement(26584..28524)
                     /gene="csrD"
                     /locus_tag="RHV13_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HDV1240768.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            28676..29650
                     /locus_tag="RHV13_001215"
     CDS             28676..29650
                     /locus_tag="RHV13_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDV1240769.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            30628..31098
                     /gene="accB"
                     /locus_tag="RHV13_001216"
                     /gene_synonym="fabE"
     CDS             30628..31098
                     /gene="accB"
                     /locus_tag="RHV13_001216"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDV1240770.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            31109..32458
                     /gene="accC"
                     /locus_tag="RHV13_001217"
     CDS             31109..32458
                     /gene="accC"
                     /locus_tag="RHV13_001217"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDV1240771.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            32567..32809
                     /locus_tag="RHV13_001218"
     CDS             32567..32809
                     /locus_tag="RHV13_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HDV1240772.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            32799..34250
                     /gene="panF"
                     /locus_tag="RHV13_001219"
     CDS             32799..34250
                     /gene="panF"
                     /locus_tag="RHV13_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HDV1240773.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWILVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            34262..35143
                     /gene="prmA"
                     /locus_tag="RHV13_001220"
     CDS             34262..35143
                     /gene="prmA"
                     /locus_tag="RHV13_001220"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDV1240774.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGADFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSERLELYLPKDQ
                     PEAMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
BASE COUNT         8522 a         9374 c         8868 g         8508 t
ORIGIN      
        1 aataatcata agcgcatatt ttttaatgaa aaatcacctc acctacaatt aaaaacacga
       61 catccgcacc ataaatagac ttgcaaaaaa tataacatca ttgttttcaa tctgccgttt
      121 atgggattga ccgttttctt ttgacacgga gttcaacaat gttcggcata attatatctg
      181 tcatcgtatt aattacgatg ggctatttga tcctgaaaaa ctacaaacct caggtggtgc
      241 tggctgccgc aggtatcttc ctgatgatgt gcggtgtctg gttagggttc ggtggtgtac
      301 tcgatcccac caaaagcagc ggctacttga tcgtcgatat ttataatgaa atcctgcgca
      361 tgctgtccaa ccgcattgcc ggattggggc tgtcgattat ggcggtgggc ggttatgccc
      421 gctacatgga gcgcacagga gccagtcgcg cgatggtgag cttgttaagc cgcccgttaa
      481 aactcattcg ctcgccgtat attattctgt cggcaactta cgtcatcggc caaatcatgg
      541 cgcagtttat taccagcgcc tccggtctgg gtatgttgct gatggtcacc ttatttccga
      601 cgctggtgag tctgggagta agtcgcctct ctgcggtggc ggttatcgca accacaatgt
      661 ccattgagtg gggaattctg gaaacgaact ccatttttgc tgcccaggta gcgggaatga
      721 aaattgccac atacttcttc cactaccagc ttccggtcgc ctcttgcgtc attatctcgg
      781 tggcgatctc ccactttttc gtgcaacgcg cttttgacaa aaaagataaa aatatcaatc
      841 acgaacaggc agagcaaaaa gctctcgata atgtcccgcc gctctattac gccattttac
      901 ctgtgatgcc gttaatcctg atgctcggct cgctgttcct cgcccacgtc gggctgatgc
      961 agtcagaact gcatctggtg gtggtgatgt tactgagttt gacggtgacg atgtttgttg
     1021 agttcttccg caagcataac ttgcgcgaaa ccatggacga tgtgcaggcg ttttttgacg
     1081 gcatgggtac gcagtttgcc aacgtggtaa cgctggtggt cgcgggtgaa atatttgcga
     1141 aaggcttaac gacgattggc actgtcgatg cggttatcag gggtgcggag cattctggtc
     1201 tgggcggtat tggcgtgatg attattatgg cgctagtcat tgccatttgt gccattgtga
     1261 tgggctcagg caatgcgccg tttatgtcat ttgccagtct tattccgaat atcgcagccg
     1321 gactacatgt accagcggtt gtaatgatta tgccaatgca ttttgccacg acgctagcac
     1381 gcgcggtttc gccgattact gcggtggtag tcgttacgtc aggaattgca ggcgtttcgc
     1441 cttttgcggt ggtgaagcgg acagcgatcc ccatggcagt cggtttcgtg gtgaatatga
     1501 ttgccacaat cacgctattt tattaagcca ttaaaaagac aaaacaggcc gccagggcct
     1561 gtttttgttt tacttcacaa cgcgtaatgc cggtcgacca ccgcgtggtg gctgcggtgg
     1621 ctcatcgtca ggatgagtgt catcatcgtg atctggcttg tcgccatcaa taaccgacat
     1681 aacggtttcg ttgtctgccg atgcctcttc atcattcatg atgctggcat cttcatcgta
     1741 ggcagcttca ggttcaaaca tcgtgccagc gccattttca cgggcgtaga tagccagtac
     1801 ggcagccagc ggcacagaaa cctgacgcgg aatgccacca aagcgcgcgt taaagcgcac
     1861 ctcatcattc gccagttcca gattgccgac agcacgcggc gcaatgttga gtacgatttg
     1921 cccgtcacgc gcatattcca taggaacctg cacgccaggg agcgtcacat ccaccaccag
     1981 gtgcggcgtg agctggttat ccagcaacca ctcatagaat gcacgcagca gatagggacg
     2041 acgtggtgtt agctgtgaca aatccataca gattaactcc tgcccagacg catttcacgt
     2101 tctgcttcag ttaaagaagc aaggaaagag tcacgctcaa agacgcgggt catatagcct
     2161 ttcagctctt tcgcacccgg gccgctgaac tcgatgccca gttgcggcaa acgccacagc
     2221 agcggagcaa ggtagcaatc gaccaggctg aactcatcgc tcaggaagta cggcttctga
     2281 ccgaagaccg gcgcaatcgc cagcagttct tcgcgcagtt gcttacgtgc ggcatctgct
     2341 tcagaagctg aaccgttgat gatggtgttc atcagcgtgt accagtcttt ttcgatgcga
     2401 tgcatgtaca gacggctttc accgcgagct accgggtaaa caggcatcag tggcggatgc
     2461 gggaaacgct catccagata ttccataatg atgcgagatt cccacagggt cagctcacga
     2521 tccaccaggg tcggaacgct ctgattcggg ttgaggtcaa tcagatcctg aggcggattg
     2581 tccttttcca cgtgttcgat ctcgaaactt acacctttct cagccagcac aatgcggacc
     2641 tgatggctat agatgtcagt aggaccggaa aacagcgtca ttaccgaacg tttgttggca
     2701 gcgacagcca tgaaaacctc caggtatagt cagaattttt actgctacca gccaccaggt
     2761 ggccagtcag aagttgtgtt acccagtaag gaacgactct ctttgttcga aaatcaaaca
     2821 aaaaatgagc aatacccgac atttgggcag aaaattggat gatagtttac cagattttgc
     2881 gacctttgtg gtgagtcgat gccggaaatg gggaaaaaga gatgcgcttt agtctgaaat
     2941 agttgactta gacccttatt ggcgatgtgg tttttgtttt gcctgtctgt cagacggcag
     3001 caaaaagcaa ctttccattt tttacgctga ttcagaattt aaccataaaa aaacccgccg
     3061 aagcgggttt tttcgcaaat tgttttctgc cggagcagaa gccaattaac gtttggagaa
     3121 ctgcggacga cgacgtgctt tacgcagacc gactttctta cgttcaacct gacgagcgtc
     3181 acgagtaacg aagccagctt tacgcagttc agaacgcagg gactcgtcgt attccatcag
     3241 agcgcgggtg ataccgtgac ggatcgcacc agcctgacca gagataccac cacctttaac
     3301 ggtgatgtac aggtccagtt tctcaaccat gtcgaccagt tccagcggct gacgaactac
     3361 catacgggca gtttcacgac cgaagtactg ttccagagaa cgttggttga ttacgatttt
     3421 accgttgccc ggtttgatga aaacgcgagc tgcggaactt ttgcggcgac cagtgccgta
     3481 gtattgattt tcagccattg cctataatcc cgattagatg tcaagaactt gcggttgctg
     3541 tgccgcgtgg ttgtgctcgt tacccgcgta aactttcagt ttacggaaca tagcacgacc
     3601 cagcgggcct tttggcaaca tgcctttaac cgcgatttca atcacacgct caggacggcg
     3661 agcaatcatc tcttcaaagg tcgcttgttt gataccaccg atgtggccgg tgtggtgata
     3721 gtacactttg tcagtacgct tgttgccggt tacagcaact ttgtcagcgt tcagaacgat
     3781 gatgtaatca ccggtatcta cgtgcggagt gtattccgct ttgtgcttac cgcgcaggcg
     3841 acgagccagt tcagtagcca gacggcccag agttttaccg gtcgcgtcaa caacatacca
     3901 gtcgcgtttt acggtttctg gtttagctgt aaaagttttc attaaaagct tacccaataa
     3961 atagttacac gttggtgaac acccaaacgt cttcaattgt tgaggttcac acgacaaagt
     4021 ccggcaaacc taccccttcg aatagcctat gccagcacac aaaaagtttt gggaaaaaaa
     4081 ctttcttgta acgtggggtc gcaggattat agagaagtcg gggtcaaaga tcgacccctt
     4141 tttgtgattt gtgccaggtt ttaacccgct aaatgctcgc gcttcagata ctcttcgctt
     4201 tgcatctctt gcagacgtga caggcaacgc tggaactcaa atttaagtcg ctcgccctga
     4261 taaatttcat acagcggcac ttctgcactc accactaatt tgacatggcg ctcgtaaaac
     4321 tcatccacca gcgcaataaa gcgccgcgct tcgctctcca tcaaccgcgt cataactggt
     4381 acatcaaaca acatgaccgt atgaaagaga cgtgagagcg caatatagtc atgctgactg
     4441 cgggcgtcga cgcacagcgt agtaaaagag accgccagcg tctggttctc gacgcccatt
     4501 gtcgctaatg gccgatggtt gatttctaac gtcggtgaat tttctcgttt cccccccgcc
     4561 agcgccaacc atagtttatc catttgcgcc cgggtttcat cgtggagtgg cgaaagccac
     4621 agatgcgcct gagtgagtgt acgcagacga taatcaacac cagcgtccac gttcattaca
     4681 tcacaatgct gtttaatggc atcgattgca ggcagaaaac gcgcacgttg caagccattt
     4741 cgataaagtt cgtccggcgg aatatttgac gtcgctacca gggtaatacc gcgggcgaac
     4801 agggctttca tcaggccgcc aagtagcatg gcatcggtaa tatcagaaac aaaaaattcg
     4861 tcaaaacaga gcacgtcagt ttctgcttta aagcgatcag caataatttc cagcggatcg
     4921 gtctgcccct gtaaggcagt tagctcttca tgcacccgca gcataaaacg gtgaaagtgc
     4981 aggcgctgtt tccgctctcc cggcaggctt tgatagaaaa ggtccatcag ccaggttttc
     5041 ccgcgtccta caccgcccca catatataag ccacgcactg gcgtatgctt tgtgtcttcg
     5101 cgtttacccc acagcttacc gacccgcgcc attagcccac tcgtcctggg ggctggtggc
     5161 gtgctattga tgagttcctg ataaataatt tccaggcggc tgacggcctc tttttgaacg
     5221 tcgtcgggtt gatggctgcc ttcattgagc gccttcaggt attgcgatgt tggggtaacg
     5281 ctttgcatga tcttattgtt attccttgaa taatcggtgc gccgttgttc acggttgacg
     5341 aaaaaaaggc cgttctacac tacgcgatat gcagtcggga ttccacttct gtggaattaa
     5401 cggttatagt ggcataatca gccgcaggca tggagcctga agccaacacc ctacggaaac
     5461 aaaagacaac gggagatgtt catgacctgg gaatatgcgc taattgggtt agtcgtcggc
     5521 atcattattg gtgctgtggc catgcgtttt ggtaatcgta aactacgcca gcaacaggcg
     5581 ttgcagtacg aactggaaaa gaataaagct gaactggacg agtatcgcga agagctggtt
     5641 agccactttg cccgcagcgc ggaattactg gataccatgg cgcacgacta tcgccagctg
     5701 tatcagcaca tggcgaaaag ctccagcagc ctgctgccgg aactgtctgc tgaagcaaac
     5761 ccgttccgta atcgtctggc agagtctgaa gccagcaacg atcaggcacc ggtgcagatg
     5821 cctcgcgact attctgaagg cgcatccggc ctgctgcgta ctggcgcgaa gcgcgactaa
     5881 tttatttttc gggcgcagcc attgcgccct cctcttctct ccctccccga ctatcattta
     5941 atctggtgtc tcattgttag ccgtctgaaa attcaataac atcaaactgt tttgaatctc
     6001 ttttcttatc attcaggtac gagagcagga ataatgaaaa aacaaaccca gctgttgagt
     6061 gcattagcgt taagtgtcgg gttaactctc tcggcgtcat ttcaggccgt cgcgtcgatt
     6121 ccaggccagg ttgccgatca ggcccccctc cccagtctgg ctccaatgct ggaaaaagtg
     6181 cttccggcag tggtgagcgt acgggtggaa ggaacggcca gtcagggaca gaaaatcccg
     6241 gaagaattca aaaagttttt tggtgatgat ttaccggatc aacctgcaca acccttcgaa
     6301 ggtttaggct ccggtgtcat catcaacgcc agtaaaggct atgtgctgac caacaaccat
     6361 gtgattaatc aggcacagaa aatcagtatt cagctcaatg atgggcgcga gtttgatgca
     6421 aaactgattg gtagcgatga ccagagcgat atcgccctgt tgcaaattca aaacccgagc
     6481 aaattaacgc aaatcgctat tgccgactcc gataaattgc gcgtcggtga ttttgccgtg
     6541 gcggtcggta acccatttgg ccttgggcaa accgccacct ctggcattgt ttccgcatta
     6601 ggacgcagcg ggttgaatct tgaaggtctg gaaaacttta tccagacaga tgcttccatt
     6661 aaccgcggta actccggcgg tgcactgtta aaccttaacg gtgagttaat tggcatcaac
     6721 actgcaatcc ttgcgcctgg cggcgggagc gtcggaattg gatttgccat ccccagtaat
     6781 atggcgcgaa cactggcgca gcagcttatc gactttggtg aaatcaaacg cggtttgtta
     6841 ggcatcaaag gcaccgagat gagtgccgat atcgccaaag ccttcaacct tgacgtgcag
     6901 cgtggcgcgt ttgtcagcga agtgttgcca ggttctggct cggcgaaagc gggcgtcaaa
     6961 gcgggcgata ttattaccag cctcaacggc aaaccgctga atagctttgc tgagttgcgc
     7021 tctcgtatcg cgaccaccga gccgggcacg aaagtgaagc ttggcctgct gcgtaacggc
     7081 aaaccactgg aagtagaagt gacgctcgat accagcacct cttcgtcggc cagcgctgaa
     7141 atgatcacgc cagcgctgga aggtgcaacg ttgagcgatg gtcagctaaa agatggcggc
     7201 aaaggtatta aaatcgatga agttgtcaaa ggaagcccag ctgctcaggc tggcttgcaa
     7261 aaagacgatg tgatcattgg cgtcaaccgc gatcgggtga actcgattgc tgaaatgcgt
     7321 aaagtgctgg cggcaaaacc ggccatcatc gccctgcaaa ttgtacgcgg caatgaaagc
     7381 atctatctgc tgatgcgtta atgtcgtaaa ccgggcatca ggctgacgtg tgatgtccgg
     7441 ttaactcgtg gtatgctgct gccgttccct tttttaatga cgcctccatc atgtttgtga
     7501 agctcttacg ttccgttgcg attggattaa ttgtcggcgc tattctgctg gttgccatgc
     7561 cttcgctgcg cagccttaac ccgctttcca ctccgcaatt tgacagtacc gatgagacgc
     7621 ctgccagtta taatctggcg gttcgccgcg ctgcgccagc ggtggttaac gtttacaacc
     7681 gtggtttgaa caccaactct cacaaccagc ttgagatccg caccctggga tccggtgtaa
     7741 tcatggatca acgcggttat atcatcacca ataaacacgt catcaacgac gccgatcaga
     7801 tcatcgtcgc cttacaggat ggacgtgtat ttgaagcatt gctggtggga tctgactctc
     7861 taaccgatct ggcggtactt aaaattaatg ccactggcgg tttacctacc attccaatta
     7921 atgcacgtcg cgtaccgcac attggcgacg tggtactggc gatcggtaac ccgtacaacc
     7981 tcgggcagac cattacccag gggattatta gtgccacggg tcgaatcggt ctgaacccga
     8041 ccgggcggca aaacttccta caaaccgatg cttccattaa ccacggtaac tctggcggcg
     8101 cgctggtgaa ctcgctgggc gaactgatgg gcattaacac gctgtcgttt gataagagta
     8161 acgatggcga aacgccggaa ggtatcggct ttgcgattcc tttccagtta gcaaccaaaa
     8221 ttatggataa gctgatccgc gatggtcgcg tgatccgcgg ctacattggt atcggcggac
     8281 gcgagatcgc accactgcac gcgcagggcg gtagtataga tcaactgcaa gggatcgtgg
     8341 ttaatgaagt gtcacctgac ggcccggcgg cgaatgcggg tattcaggtc aacgatctga
     8401 ttatttcggt ggataacaaa ccggccatct ctgctctgga gacgatggat caggtggcag
     8461 aaattcgccc tggttcggtg atcccggttg tagtgatgcg tgatgataag cagttaacgc
     8521 tgcaggtcac cattcaggaa tatccggcaa ccaattgagt cgtgcgctca aaacaaaaaa
     8581 ccggagtctg tgctccggtt ttttattatc cgctaatcaa ttacttatta acgaactctt
     8641 cgcccagggc gatatctttc ttgagcgtat ccagcatacc ttccagcgcg ttctgttcaa
     8701 atgcgctcag ggtaccgata gatttacgct cttccacgcc gtttttaccc agcagcagcg
     8761 gttgagagaa gaaacgggcg tactgaccgt cgccttcaac gtaggcacat tcgacaacgc
     8821 cttgttcgcc ctgcagtgca cgaaccagag acagaccaaa acgtgcagct gcctggccca
     8881 tagacagggt tgcagacccg ccaccggctt tcgcttcaac cacctcagta cccgcgttct
     8941 ggatacgttt ggtcagatca gccacttcct gctcggtaaa actaacgcca ggaacctgtg
     9001 acagcagcgg cagaatggta acaccagagt gaccgccaat aaccggcact tcaacttcgc
     9061 ctggctgttt gcctttcagt tccgcaacaa aggtgttgga acgaatgata tccagcgtgg
     9121 taacgccgaa cagtttgttt ttgtcataaa caccggcttt tttcagcact tcagcagcaa
     9181 ttgcaactgt ggtgttaacc gggttagtga taataccaat gcacgctttc gggcaggttt
     9241 tcgcaacttg ctgtaccagg tttttcacga tgccggcgtt aacgttaaac aggtcggaac
     9301 gatccatacc cggtttacgc gctacacctg cagagataag aacgacatct gcgccttcca
     9361 gcgccggagt cgcatcttca ccagaaaaac ctttgatttt cacagcagta gggatatggc
     9421 tcagatcgac agccacaccg ggagtcactg gagcgatatc atacagagag agttctgaac
     9481 ctgaaggcag ttgggttttt aacagtagtg caagcgcctg gccaataccg ccagcagcgc
     9541 cgaggactgc gactttcatc ctaaactcct tattatattg ataaactaag atatgttgct
     9601 ccgctgccgc gaccttaatc cacaaaattg ccgtttacaa tgaccacatc tcaagaatgt
     9661 gtagtcacgc aagtttagcg tttatgcatt taattgccgt aatcaggaac ctaacttacg
     9721 taattaacag tcgcttatgc attagcgcaa cattccaaca ggtggtgaca atatacccta
     9781 ccgttcagcc aaaacaacat caatttgata acaattaatt tacttttaag cagaatttgc
     9841 atgccgtgac gcaggcatgt ttctcaataa cgaaatttga taaaatcccg ctctttcata
     9901 acattatttc agccttcttc agggctgact gtttgcataa aaattcatct gtatgcacaa
     9961 taatgttgta tcaaccacca tatcgggtga cttatgcgaa gctcggctaa gcaagaagaa
    10021 ctagttaaag catttaaagc attacttaaa gaagagaaat ttagctccca gggcgaaatc
    10081 gtcgccgcgt tgcaggagca aggctttgac aatattaatc agtctaaagt ctcgcggatg
    10141 ttgaccaagt ttggtgctgt acgtacacgc aatgccaaaa tggaaatggt ttactgcctg
    10201 ccagctgaac tgggtgtacc aaccacctcc agtccattga aaaatctggt gctggatatc
    10261 gactacaacg atgcagttgt cgtgattcat accagccctg gcgcggcgca gttaattgct
    10321 cgcctgctgg actcactggg caaagcagaa ggtattctgg gcaccatcgc tggcgatgac
    10381 actatcttta ccacccctgc taacggtttc accgtcaaag acctgtacga agcgatttta
    10441 gagctgttcg accaggagct ttaatctctg ccccgtcgct tctggcggcg gggaaaatgt
    10501 tgcttatccc tctcaatccc ctgctttccc ctgcgattca tttaacgaat agtgcgtttt
    10561 accgcgacat atcattcaca tggtgaatac atacggtaaa aaaagcacat tatgcaaaat
    10621 tcattatctg attgaaaaaa ctggtattta taataaataa ccgtgttttt aatacttttt
    10681 tgttattaaa attcacattt ttaacactta gtatcaactg aaacagttag cgcggtatta
    10741 attagcccaa taattagtgt atacttgatt ttgtgatatg ggtcacgaaa caaaggccca
    10801 gctaaaagat tatgtcgagg taaaaatcat gaaaatcaaa accactgttg ctgcattaag
    10861 cgtactttct gttctctctt tcggtgcatt cgctgccgac tccattgatg ctgcacaagc
    10921 acaaaatcgt gaagcaatcg ggaccgtatc cgtaagtggt gtggcgtctt cgccaatgga
    10981 tatgcgtgaa atgctgaaca aaaaagcgga agagaaaggc gcaacggcct accagattac
    11041 tgaagctcgt agcggtgaca cctggcacgc tacggctgaa ctgtacaaat aaaccctcat
    11101 cgtcttgtcc gacgatattg cccccggttc gggggctttt tttgcgctaa tgacgaacat
    11161 taaaacgcaa atgcccttcc agctcttcct ctgcctcatc aaacagcaat attaacgcgc
    11221 caaaacggcg acgcgttttc tctcccagat gaacaaattc aatctcaagt ggtagcggca
    11281 ggacatcgtt cattaacaca tcccatagtg agtcgagatc gcgtacctta tctttcgcca
    11341 gaccaaaggc ttggctgaag tcgcgataaa aatcctcctg actctcaatc tcatcaaaat
    11401 caaaggtata aatattcatc tgttgccacc gtcacgtttc gcggcgagtt ctgccgtgca
    11461 tcgctaagta tagccatgaa aaaccgacgc ttttggcgtc ggttttgact taactatcgg
    11521 tcaacgcatg ttgatactta tggagcatcc ccgccagccg atgaaccggt tccgtcacct
    11581 gcggtggcgc ttgccagatg cgtagctttt cctggtagat ttccagttct tccagcaact
    11641 gaccaaagta ccgacgacgt ttatcatcgc tacgggcaga tatcacatgg tctgctgtgc
    11701 ggcgcatttg tcggtgaaac gccgataaat cctcgttaac cgggatcggc gcatcacgga
    11761 ggcgctggtg ggcgataatc atcgtcagcg ccaggcgaaa tttcggcaaa tcccctggga
    11821 acttattcat cagcaaaaac agctgctgat aaagtgccgg gaggtggttc tctttacgac
    11881 gtgccacatt ggtagtcatc gcggaaacag cggcagaaac aaactgatta agcagtacac
    11941 gtccagtcct gtcgcgcgat ttatcccgca ccagcaaaat aacggtgaac gcgagcacac
    12001 agccgacgat ttgccctaat gcgctgtcga gaaactgact gaaatggaaa gtcatcgggt
    12061 tatccagcac gataatattt atggtgctgg ccagtgcccc catcgagccc agtcgccgtt
    12121 tctgtacttc tataccgagg aaaaatccca gcactgccag gctaatacac agcagcaaca
    12181 tgctctgttg ggtattaggg ataatcacca aaaagtagag caaccctaac ggcagcgcgg
    12241 ccagcgtccc gtagataaag tcgatcgcca ccatgcgtgg attcggcaaa cgcattgcca
    12301 gtgacgtcac gaccgcaatc atcaccattg caccactgcc ggaagtccag cccgtccaca
    12361 gccagaaaag cgttcccaga atgcaggaaa gtgtggttcg ccagaagtta accatcgcat
    12421 gatgacgttc ggctgactct actttgactt ccggttcgcc ttgcaggatc tcttcttcgg
    12481 tggcgttgat ttttgtgtta ctgataacgc cgcgcttgag aagctgataa cgcgttgccg
    12541 ccgcgaccca gctataaatg gtgacaggcg tttcccgttc cccggtccag gcgataactc
    12601 tccgcaggcg tttgagctgc ttgtggacgt cctgcgcggt ttctaccggc gtgtcaaaaa
    12661 attcgcggaa agtatcagtg atcaattccg ggcgcgtatt ctgaataaga taagtttcgc
    12721 aggattgggt aatcagcgtc agcgatagcg tattgatcgc ttttaaacgt cgattggccc
    12781 gcgcccagcg ggaagattcc atattcaggt tgctgcgcat gccttgtagc gccgtggtgc
    12841 gtcgcaccag gtcgccccag gctttatcga caacttcacc atcgccatgc ttgatacaga
    12901 gttgcattaa ttgatattgc gcgaccagca aactttccag ctctcgatcc acttcttgtt
    12961 tgatcgatcg cggagaaaag agcagatccg ccataatcgc acacacaata ccgataacga
    13021 tctcgctaca acgttcgacg gcaaactgcg gcgtaagaag cggttccggc tgaatggtga
    13081 tcacaatgat cagcgcggta taaccagcca gcccccacgc atacgagttt tctattcgta
    13141 ccagcgagga tatccaggta caaaaaccgg cccagataca gcacaccaga atcatcaata
    13201 atggtgcgcg gatcatcgca atgatgatca ccagtccggc aatacagcca ataaatgtgc
    13261 cgatgatgcg caaaaagcca cgatagcgaa tagcgccaga atacggttca cctcccgcag
    13321 caaaggccgg accggcggca acaatcgccg ctgtcagtac cgcccagcgt ggcgtttcca
    13381 gctggaagtg aaagccaaca aacagcgcca gtacaatggc ggtcgccagt tttaccgcaa
    13441 agcgaatatg ttggttagca atggagaaaa tacccatcgt gattaaccaa actcacgcag
    13501 gcgatgggcc attttacgga agaacgaatc ctggctttcg tcgcgatctt gtttgccagt
    13561 gaccaccact gtagcagtgg tgcccgcagg ccagatgttt tcttgctggt tgtcgagacg
    13621 aatacgaacc ggaacacgtt gcgcaagacg cacccattca agattagagt ctatagtcgc
    13681 catccctttg tcgtcacgcg tgctgctggc gttggtgacc cctgcggcaa cactatcaac
    13741 agtccctttc agcactttgt tactgccaag cggcgtgatc tctgcacgat accccggacg
    13801 caccccttcc agcttagttt cttccatata ggccagtaca tagaaggagt tctgtttcac
    13861 cagcgcaacc gccgttgatc ctcgagtaat aaactcaccg gtatagacgt tgaggttggt
    13921 cacccagcca tctgctggcg cgcggatcac cgtgcgttca agatccagtt ttgccagatc
    13981 gcgggtcgcc tgcgctttcg ctaactgatg cagaaccgtt tgtagtacgt tgttggcctg
    14041 gtcgatctct tcgcgagaca tcgcctgcac accgagacgg ttacgacgtc cggcctcctg
    14101 gcgtttctcc tgtgccagta cctgataata agcaacatcg gcttgcgctt cctcaagcgc
    14161 cttttgatag cgcggctggt cgatggtgaa cagtatctgt ccttttttca ccagctggtt
    14221 atcatgaaca ttcacctggg taatgagtcc agaaacgtcc ggcgcgatcg caacgacgtc
    14281 agcgctaaag cgcgcgtcac gcgtccaggg ggattcggtg taatagaccc aggcattaaa
    14341 aattgcgatg aaggccagaa tgactaatac gaccgtgatg gccgtacggg agaattttct
    14401 tattagtgtt ttcacttcaa cctcaaacga acagtcgcga tatcaaataa aacaagcagc
    14461 aatagagcgc ggtgttgaac aacgccggat gccagacaaa gtcgtagata cctgttggca
    14521 caagtacccg gcgcaccagc cagaaaatcg ccagtgataa aagcaattca aaaaatatcg
    14581 gtgggaagga cagcccaaac accacgataa cgggaaacag actcatgttg accttggttg
    14641 tatagagaga gcaggcgtta ttattttcag catctgtcgc cgcagagaag ggcatggaaa
    14701 gccgggcgag agcaacattg ctgtagattg atatttaata tattagcgta actgttatgc
    14761 tgttatctat attatgtgat ctaaatcact tttaagtcag agtgaataat ggaacgacta
    14821 aaacgcatgt cggtgtttgc caaagtagtt gaatttggct cttttaccgc cgccgccaga
    14881 cagctacaga tgagcgtttc gtccatcagt cagacggtgt caaaactgga agatgagttg
    14941 caggtaaagc tgttaaaccg tagcacacgc agcattggcc tgaccgaagc cggtagaatt
    15001 tactaccagg gctgccgtcg tatgcttcat gaagtgcagg atgttcatga gcaactgtat
    15061 gccttcaata acacccccat cgggacgcta cgcattggct gttcttcaac tatggcacaa
    15121 aatgttctcg ccgggctgac agccaaaatg ctgaaagaat acccaggttt gagcgtcaat
    15181 ctggttaccg gaattccagc ccccgacctg attgccgacg gtctggatgt ggtgatccgc
    15241 gtcggcgcgt tgcaggattc cagcctgttt tcccgccgtc ttggcgcgat gccaatggtg
    15301 gtgtgcgccg cgaaaagcta tctcacacaa tacggcatac cggaaaaacc cgccgattta
    15361 agtagccatt catggcttga atacagcgtg cggcccgaca atgaatttga actgattgca
    15421 ccggaaggga tctcgactcg cctgatcccg caagggagat ttgtgactaa tgatccgatg
    15481 acgctggtgc gctggctaac ggcgggtgcc gggatcgcct acgtgccgct gatgtgggtg
    15541 atcaacgaga tcaatcgtgg ggagctggag atcctgctgc cgcgttatca gtcagatcca
    15601 cgcccggttt atgcgttata taccgaaaaa gataagctgc cgctgaaggt acaggtcgtg
    15661 atcaactcgc tgacggatta ttttgttgag gtcggtaaat tgtttcagga gatgcacggg
    15721 cgcgggaaag agaagtaatt catattgtac tgttacgttg tacaaacctg tgccaacggg
    15781 ttcccctcac cctaacccca gaggagcgag gggaccgttc gtgcacgtag aaagattaat
    15841 tatccttctg aaaatactga aattattacg cagtaccgcc aacagtcagg ttatcgactt
    15901 tcaacgttgg ctggcccacg ccaaccggca aactttgccc ttctttaccg cagacaccca
    15961 cgccgttatc cagtttcagg tcgttgccaa ccatcgaaat ctgctgcatg gtttcgatac
    16021 cggaaccaat caacgttgcg cctttcaccg gcttcgttac tttaccgttt tcaatcagat
    16081 atgcttctga agtggagaaa acgaatttgc cggaggtgat atccacctga ccgccaccaa
    16141 agttcggtgc atagataccg tactcaacgg attcaataat ttcctgcggg gtcgatttac
    16201 ccggcagcat ataggtgttg gtcatacgcg gcatgggcag atgggcgtag gattcacggc
    16261 gaccgttgcc agtcggcgtc atccccatca aacgcgcgtt gagtttatcc tgcatgtagc
    16321 ctttcagaat gccgttctca atcagcacgt tgtactggcc tggcgtacct tcgtcatcaa
    16381 tcgccaccga acctcggcga tcgaccatcg tgccatcatc aaccacggta cacagttctg
    16441 aagccaccag ctccccgacc tgcccactaa atactgaagt gccacggcgg ttgaagtcgc
    16501 cttccagacc gtgaccaacc gcttcatgca acagcacgcc cggccaacct gcgccaagta
    16561 ctaccggcat ggtgcccgct ggtgcagcaa cggcagaaag attgaccagc gccatacgca
    16621 ctgcttcttt tgcccatgca tccgcacgga cttcgccgtc gagatcggca aggaagaact
    16681 cataaccaaa acgaccgccg ccgccactgg caccgcgttc gcgtttgcca tcttcttcga
    16741 cgagaacgct cacggaaaga cgcaccagcg gacggacatc cgccgctagc gtgccgtcgg
    16801 tggccgcaac cagaattaat tcatagacac cactgaggct ggcagtcact tcctgtacgc
    16861 gcttgtccgc ttcgcgagca accttatcga cgcgacgcag gatatccagc ttctcttcac
    16921 ggctcatgct ttgcagcgga tctaccgagg tatacaacgg gctatgctct accgcgccca
    16981 gcgtctgtac tttgccatca ccactatcac ggacgatggt gcgcgccgct tgcgcactct
    17041 gttccagcgc cagcaggctg atttggtcag cgtaagcaaa tccggttttt tcaccgctga
    17101 ttgcacgcac gccaacgccc tgatcgatgt tgtaagaacc atctttaata atgcggtctt
    17161 ctaaaaccca ggattcgtga tagctcgact gaaaatagag atcgccataa tcaaggcgac
    17221 gttcggccag ttgaccgagg atcgcgaaca agtcctgatg tttcaggccg ttcgccgcta
    17281 gcaattgttc acttaccagg ttcagactca tcgtttttgc tactcgttag ttactgcagt
    17341 agaggatttt ttacggctgc cggatgcggc gtgaacgccc tatccgacct acgattatgt
    17401 tccggtttgt aggcctgata agacgcacag cgtcgcatca ggcaacggct gtcggatgcg
    17461 gcgtaaacgc cttatccgac ctacggttat gttccgtttg taggcctgat aagacgcaca
    17521 gcgtcgcatc aggcaacggc tgccggatgc ggcgtaaacg ccctgtccgg cctacggtta
    17581 tgttctggct cgttggcctt tggcaacgat tatcctatga gattggggca attacgcgcc
    17641 ctcgtcaaat cattgcgctt tttctttacg cggttggcgc aacacttcgt tgatttgcgg
    17701 atcgtccagc ggacccgaaa tgtgatagcg caaaatggag actttgctcc acagcggccc
    17761 cagcacttta ctggctgcaa atactgccgc gccaacaatg gggttaaccg caaaagccgc
    17821 agccacgccc accgtcgcag aaatctctgg tgcgacaacc gcttccatat tcaggtcgcg
    17881 acgtaccaga tttaccgacc ctttcatggc gatatccgcc tccaggccat ccaccagcgt
    17941 gtcgtcggtg tgcataacgc cgtctttaat ccacgcggtg ctgcgaatgg agtcaaaata
    18001 gaacccttcg ccaaaagtgt ctctgaaatc aaaacgcagc ttacgcatca gggcatctac
    18061 gctcaataag cgcagcaatt gcccagcatg tccggtattg atttcggtaa tttcgccttt
    18121 acccagttga gtatgaatga tgccattcaa cgtcgcttca tctggctgcc agggtgtttt
    18181 acgccagtgt aaatcgtaat ccacattaaa tgacgactgc cgtatgggcg tcgtgacacc
    18241 aaaaaattct gcggcggcat caattttctg cccgcgcagt tttcctttca gcgaggtacg
    18301 ttcattcccc ggattattaa cccattcacc atcggcagta agccgcgaga aaccagtatc
    18361 aatcagtcca ttggtcagcg ttaacgtatc gccagaaatg gtgagatcac tgtcaatacg
    18421 accgaatttt tgcccccaga accagcactc tgtgcatcgt atttgtgcgt ccggccagcc
    18481 acggaagtta atgcgctccg ttgtcgggaa aggtgatgac ggtgttgaat caccacgagt
    18541 tttcgccatg ctcgggttgt aataaagata tttgatattc gccagccacg gcgcgttatt
    18601 acgcatcgcc agcgtggcgt tgatttcacg cccttgcgcc tcaacctggg tgccatttgc
    18661 cgtcggttgc gaaacaatac tcaggttatt ccattgctga tttcccagtg acaacatagg
    18721 cgtacgtaac gttatgtgtt gtgggaaact cgctgcacca ccgacactct ccgcagcgcc
    18781 tttctggaac agggccagcc actcggcacc attcatcggc ggcatattga gttcaacgcc
    18841 actttgttcc ggcaacggcg ggagcgtttt actgtctgcc gcccaaatag cacgatcgag
    18901 cgtcagcttt tgaccgagca accatcggct attgaaatga ttatctgcac cagcctgtcc
    18961 ggttaattca aagctgttga gattgccatc aaccttaacg tttaccgcta gtggttcacc
    19021 cgcaggtttg gctaacggtg aaggtaagtg actgctcaca ttcttcagat cgccgttcag
    19081 ctctacgtta taggtcgcgc cagcatgata aggcagctca atgcccactt taccatccca
    19141 cgccacgctg ccacttaatg cttcgttcac cgctgcaggc agaacgccgg ttttcgctgg
    19201 ttgccagtta ccgttgagat tcaccgcgac ctggtaggct tttgcccctt ctttggtgga
    19261 aaaatccacg ttcaacggct gattaaacca gcttgctgtc agtggttcac tttgcagatc
    19321 gccattgata aagctgaatt taccgctcaa atttttcagg gtgctgtcga gtggtttgat
    19381 aaacagactg ttattacgca gcgtcacatc acctttcgcg gttaccagtt cgccgttcag
    19441 cgggatatca agatgtaagc gagcattcac atcgccgtcg agctggagtt cttgcagggt
    19501 cgcccccaaa gaatctttca gcggtgtctc atcaaagtaa gggccaacgg ctttacccgg
    19561 acctttaatg tcagcgtcaa tcagcagttt ttctttcgag tagtcaggga tcactgcggt
    19621 gagattactc gcgcgcacgc cgcccagatt aacgccatcg gttttcatcc ataaaccgtc
    19681 gttaataaag tccagttcaa tatcaaggtt agttaatgca ggccagtccg gctggaaggc
    19741 aaacttcgcg ttgcgcagcg gcaccagcac ttcaaactga ccttcgttgt gtttataggg
    19801 gaagagttgc ggattgccac cataaaccag cgtcgcgtta tccgcttcac cgccctgaat
    19861 cgcgccactt aagtaatcaa ccaggtcttt acccatcaag ttttccggga aatagcgcca
    19921 ggcttgtgaa ccatcatcgg tactgatgcc agccagaata cccagccagg gttcatcgtt
    19981 agcaggttgc aggtaacgaa aaccgccgcg cgcatggacg gctttggctt taacgtcaat
    20041 attacgccca tccagctgga aacctttgtc attgttcagc cagcttatag ttgcctggcc
    20101 gtcggcgatt tccagtggcg cacggaatac cgtttcgtaa ggcatctttg cctgcttcat
    20161 cgacgcggta agcaaaccat tttcaacgct gccggaaagc gtcccggaga agtgttccgc
    20221 acccggtaat aatttccatt gcttccaggc cagatcgctc cacgatgcct gaaaacgggt
    20281 cttgtctgcc gcctgaagcg ggatatccag cgccagagtg ttaatcttgc cgctcggttg
    20341 tgtggagcgc caaacatcac ccagtgcagg tgaaagtttc gcggccagcg ggcgtatgcc
    20401 ctccaggcct gccagctcca gattactggc gcgaatccgg agttcgtcac tgcgtttatt
    20461 gtctttgccg ccaacgtcct gttccggtat ccaggccagc gtcaatgctc cgctcggcca
    20521 gggtttgccg tccatcgtga tccgtgtatc gggaatagag aactgccagc ccggattttc
    20581 acgcgtaata tgcgcggtca gattatccac cgacagcgta tgcgtttgct tctcgcccaa
    20641 ccagctggca ccgccctgtt tcagccagac gtcaccgccg gttacatcgc ctttgtcgat
    20701 cgtcatccag ccttcaaggg agaactgtgc cgtttccaga gcaatattgt cctgcatcca
    20761 tttaccgagc cacggcttca ggtcgatgtc atccgcctgt agccagacgc gaccattgct
    20821 taacaacccc tcatcatcgc gcaaatccat gcgcacctgc atcacgccgt gctgtccggt
    20881 aaggctggag aggcttacca ggccttccgc acggtgtcga cgtggatcgt tcagccaggt
    20941 gagttgtggg atcgccagct cggcgcgctg accggatggc gtcagaaaac tgacttcact
    21001 gtcgcggaga tcgaaatggt caaattgacg aagaaacaga tcgctgatgt gactggcttc
    21061 cagactgtca tcgctaccac cgctggtgat aggagtgttg gtgcgaaagc gcagctgcca
    21121 gaaagtgaga tcgcgaaact gccagcgcat atgtaacagg ctctgccaga catccagcgc
    21181 cagagtaacg cgtttaaccg aaaattcgcc gccatctttt agttctacac ggatgtcgtg
    21241 tgcttcaagc gtcgggccaa aattctgcca gctggctgag agctgactgg cttctaccgg
    21301 catgccagtc gcggattcta ttttgttgag gatttccgga cgccaggcgt caagatgcgg
    21361 taaagcaata cgcaggccgc taaccagcag ggcagcgatc acaacgagcg cggctccagt
    21421 aagcagtaaa atccccggca atcgcctcac ccgtcactcc ttgtctgcta aaaatgtgac
    21481 tcaaaaaccc tttgccggat ggcggcccag catctgttta catcattacg acgtcaaact
    21541 gctcctggtt atagagcggt tcaatttgta ctttaacctg tttgccaacg aaaatttcca
    21601 cttccgccag cgagtgtgac tcttcgcctt tcaaggcttc agctactgcc ggagaagcat
    21661 agaccaggaa acggtcggag tcgtaagcat ggtggacacg aacaatctcg cgcatgattt
    21721 catagcatac cgtttccacg gttttcaccg ttccgcgacc gtggcaggtt gggcattcgt
    21781 tacacagtac gtgctcaatg ctttcgcggg tgcgtttacg cgtcatctcc accagcccca
    21841 gcgccgaaaa accattaacg ctggttttca cccggtcttt gctcaacgcc tgctccagcg
    21901 agtgcagcac gcggcggcgg tgatcttcat tattcatatc gatgaaatca ataatgataa
    21961 tcccacccag attacgcaac cgtaactggc gagcgatagc ctgcgtcgct tcaatattgg
    22021 tattgaaaat ggtgtcgtcc agattgcgat gaccgacaaa cgctccggta ttgatgtcca
    22081 cggtggtcat cgcttcggtc tggtcgataa tgagataacc accggatttc agttctactt
    22141 tgcgttccag cgctcgctgg atttcgtttt cgacatcaaa gagatcgaaa atcggctggc
    22201 gtccggtgta atgctccagc ttgctggtca tctcgggaat gtactccgag gtgaactcaa
    22261 gtaatgcttc gtaagtcagg cgtgagtcaa cgcgaatgcg gtccagttcg gcgtcggcaa
    22321 aatcacgcag aacacgctgc gccagcgcca gttcgccgta cagctgataa cgggtctgcg
    22381 ggcgcttttt acgctccata actttggtcc agacgcgttt cagataagcg gcatcggagg
    22441 ccagttcagc ctcgccaacc ccttccgctg cagtacggat gataaacccg ccctgctcgt
    22501 cgcaatactc cgcgaccact tttttcaggc gttcacgttc tgattcgctt tcaatacgtt
    22561 gggaaacccc aacgtgagaa gcccctggca taaagaccag atagcgagaa ggcagcgtga
    22621 tatcggtggt caggcgcgcc cccttagtgc caagcggatc tttcaccacc tgcaccatca
    22681 gatcttgccc ctgacgaacc agttccgaga tgtcgcgcac cgtgaattgc ttttgttctt
    22741 cacccgccac acattcggtg tgcggcatga tgtcggaagc atgaagaaac gcggctttat
    22801 ccagcccaat atctacaaaa gccgcctgca tacccggaag tacacgactt acacgaccct
    22861 tgtagatatt gcctactatt ccgcgtcgcg cctcacgttc aatatgaatt tcctgcagaa
    22921 taccgccatc aatatacgcc actcgcgttt ccgaaggcgt tacgtttact aacaattcag
    22981 ccgtcatgtt tatccctttt ctcacgcagt gcgttaaaat tacttaataa ttcatacgtt
    23041 tcaaccagcg gtaagccgac tacggcgtga tagctgccat ttatcttcct gacaaaacag
    23101 ccacccagcc cctgaatacc gtatgcacct gctttatcta acggttcacc gctggcgaca
    23161 tagcccgcga tgtcttcgtc tgttaacgtt ctgaaagtca catcggtgac caccaggcaa
    23221 tcgagaatgt gctggctgtc ggccaacgcc actgccgtca tcacctgatg ggtctgaccc
    23281 gataatttgc gcaacatctg cgccgcatgc tctgcgtcgc gcggtttctc cagcacttct
    23341 ccgttcagga taacgatagt atccgcaccc agcaccggga gatccttcgc cgtttgcgcg
    23401 acacctgccc gtgctttctc gcgcgccaga cgcacaacat actgctgcgc gctctcctgc
    23461 ggctgacgct gctcctcaat gcccgtaaca atacgttcaa aggtcacgcc aagttgcgca
    23521 agtaactcct gacgacgcgg agaaccggaa gctaaataca gagaagtcat agaaaccttt
    23581 attgcactgc aaactgctga cggactttac gcatcagcaa gaaaatccac ggccagagca
    23641 ccccattgac tacactactc cagaacactt ccggtctgaa agagacgtta atcactaaaa
    23701 actctgccca gaaaacaata atatccacca ccagcgaaag caacatgacg accagcgcct
    23761 gctgccataa tgcgaggttg cggaaaagct ggtatttcag cgccaccagg taagcaatga
    23821 tgctcatcgc caatacgcgt acgccaagcg tcgagccgct gatcagatcc agtatggcac
    23881 ccatcacaaa acctgtgccc acatttacgc gatggggcaa ggccaggatc caatacaaca
    23941 agatgagtaa cacccagttt ggccggaaaa caatcaggtt atccggccag ggcatgattt
    24001 gcagcaaaag cgcaatgagg aaagagagcc agattaccca gcgtccctgg ctacgatagc
    24061 tcgccactat tgccctcccg gcgcacgcgc aggcggttga gcaccacttt gcggcggcgt
    24121 agccctttgt ggagagcgat tagcagcggg ctgtgtcggc gcagcaggcg cagtagctgc
    24181 atttcctgtc gtgggttgct gcggagtcgg ctgagcgatc cccgttgccg gttcaggtaa
    24241 ctttggcccc atcgcgtctg gcgaaggcaa tacctgcggc atcatctgca tcagacgttc
    24301 attagcaaca cgatgcacct cttccggcgt catcgggtta gcgccgttac gatctgcccc
    24361 ccacagcagc agcagataac gcaaacgttg cagccctgca gtcggacgcg cctggatcac
    24421 agtataagcg cgctgggtat cgagttttac ggaagagaca accgcgaccg gatagccttc
    24481 cgggaaacga ccgcccagac cggaggtcac cagcacatca ccaacacgaa tatccgtatt
    24541 cgctggcaga tgctcaagct gcaaatcatc cgtacaaccg ttaccggctg caattacgcg
    24601 gatatcgttg cgcagcacct ggattggcag cgcgtgggtc gcatcacaaa tcagcagcac
    24661 gcgactggtc agtttagcga cggccaccac ctgaccaaca acacctttgt cgctgatgac
    24721 cggctggcct tcataaacgc cattaacgct acctttatcg ataacaactt gatcgctata
    24781 aggatcgtta accgtggaga taacctgagt caccattttc tgctcatcct gacgcagcgg
    24841 ggaacccagc aactcgcgca gacgcgcgtt ctcctgtttg tattgtccaa gcatcagcag
    24901 ttcactgttt ttcagcaaca gttcctgacg taacgcccgg ttttcaagtt ctagttggtc
    24961 acgcgaggcc agcgtctgcg atacgccatc cagcaattca cgaggagcat tggaaacaaa
    25021 gtagaaagga ctgacggcgg tatccatata agtacggatt tgactgaacg tccccaggcg
    25081 gctgtcggca ataataatgc cgagcgccac cagcaccgcc agaataaggc gaatctgtag
    25141 cgacgggcca cggctaaaaa ttggcttcat aagttatgcg tattctcgta tcagaccagg
    25201 cagggtaaac agacgcttcc cctgcctgca tccgattact cttcgctgaa caggtcgccg
    25261 ccgtgcatgt cgatcatttc cagcgctttg ccgccaccgc gcgccacaca ggtcagcggg
    25321 tcttcagcaa caacgactgg aatgccggtt tcttccatta acaaacggtc aaggttacgc
    25381 agcaatgcgc caccaccggt gagcaccatg ccgcgctcgg agatgtcgga agccagttcc
    25441 ggcgggcact gttccagtgc aaccattacc gcgctcacaa taccggtcag cggttcctgc
    25501 agtgcttcga ggatttcgtt ggagttcagg gtaaaaccgc gtggaacacc ttctgccagg
    25561 ttacggccac gaacttcgat ttcacggact tcatcgcccg ggtaagccga accgatttcg
    25621 tgcttgatac gttctgcggt ggcttcaccg atcagagaac cgtaattacg acgcacatag
    25681 ttgatgatag cttcgtcgaa acggtcacca ccaatgcgca cggaagagga gtaaaccacg
    25741 ccgttcaagg agataacagc aacttcagtg gtaccaccac cgatatcaac caccatagaa
    25801 ccggtcgctt cagaaaccgg caggccagca ccaattgcgg cagccatcgg ttcttcaatc
    25861 aggaagactt cacgggcacc agcgccctgc gcggattcac gaattgcgcg gcgttcaacc
    25921 tgggtcgcgc caaccggtac acaaaccaga acgcgcgggc ttggacgcat aaagctgttg
    25981 ctgtgcactt gtttgatgaa gtgctggagc attttttcag tcacgaagaa gtcggcgata
    26041 acgccgtctt tcattgggcg aatggcagca atattgcccg gcgtacggcc cagcatctgc
    26101 ttcgcgtcat gacctactgc agctacgctt ttcggtgaac cggcacgatc ctgacgaatg
    26161 gccaccacgg aaggctcatt caatacgatg ccttgtcctt ttacataaat gagggtattc
    26221 gcagtaccca ggtcaatgga caagtcattg gaaaacatgc cacgaaattt tttcaacata
    26281 ctaagggata atcctgaaag ctggggcgga aaagaaaatc cgcttacttt accaaccaca
    26341 cgcagcagcg acaaggcgca aaaatcatct gctacggtga aaattagtgc agttcgtttc
    26401 ctttgttaca aatctctgcc tgagtccaga aaggcttaat gcatcagcag cattcctcgc
    26461 ctgtttgcaa ccgcgtaagg tcattcatct gcatatgtgc tgcaacaatc tggcgagcag
    26521 acaagcacac tcccatgaga cgcagcgcgc gttattctac gtgaaaacgg attaaacggc
    26581 aggttaaacc gagtatcttt gtgaatattt tttcacgtta gtatcaagtg gctgtgagga
    26641 cgcgaaaaaa tccccttgcc cgcctgtaac accgcgctga atcagggtct gccactcgct
    26701 tcgcgaacgc acaccggtgg cgtaaacctg ggtgctggtc cctgagcagg cttccaccag
    26761 gctttgaacc agcagctggt tctccgttcg cttctcaatg tttctgacca gccccggatg
    26821 gagcttgagt aactcaacat taagttcttt gatccaactg gtgcttacca gcgtcaaacc
    26881 agcctggttg acggctaccc gtacccctaa agcattcact aaacgaataa caggttgtaa
    26941 acgactgata tgttgaccta catcggcctc tgcaagttca ataattatgc gttttcgttg
    27001 tgatttttca cattgcatta acgtatcgcg cagccaacgc tgaaaacgcg ggcgaatcag
    27061 cgactcaacg gtaacctgaa tcgccaggtt ttcctctggc cagtaacgca atagtggaat
    27121 aagacggctg atttgcagac ggtcatactc ttccgataag ccaaactgca agaccatcgg
    27181 catatactcc gccgagctaa cctcttcatt accatcaaag atgcggcaca tgagttcgcg
    27241 atgatgaacc tgaccttcgc gagtaaccgc cggtttttga taaaggcgcg ggccgccgcg
    27301 actgagcatt tgctcgataa gcgtacgcca gcgaacatta ccgcgtcctt tttcaggcaa
    27361 cgagtcatcg taaatagccc agctattgcc gccctgcaat cccgcattac gcgtggcaga
    27421 ctctgcatgt tccattacct gctcggtatc ctgaccacta cgccaggcgc agataccaat
    27481 gtggatcata tcgtcgcgat cgagcatttt attgttcggc aaggtatcga cggctttgat
    27541 taactgaccg gcgatgctct ctgcctcttt taacgtccgg tgcggtaaca gcgcagcaaa
    27601 atcactgcgg tggtaacgcg ccagcagtgc gccagggtag cgcatcataa atgtcgacag
    27661 cagattcgtc agagtgaaga actgttcttc aacctggctg tgcccccagg tatcgctcaa
    27721 catattgaaa tccggcagac gaatcatcat cacgatcccg tgggtaccta ctttctcctg
    27781 gtcttccagt aacgttgcta actgattatc gaaaaagagt cggttattga ggccggtttt
    27841 cacgtcctgg gcggcataag agcggatcag cgtatcaaga cggctgtgtt gttcgcgtgc
    27901 gttctgaatt tcacgaagca gcgtatctag cgcactgctg gttctgggcg gccattcata
    27961 gatggttccc aacacattag agccacgctc accgtttaag atacgagtag cccgggtttc
    28021 cagcaattct tgcccggcaa gttgccgttg taaccagcgt accgccagga agagcataac
    28081 aatgataaag ccaatcgccc ccgtgagcgg cgcggtagtc attaaagaat ggaaatagtt
    28141 gcccatcgga tcctgataaa ccagacgcag cgacatcccc ggatgcttta tcaacggaac
    28201 actcagttcg cgaaacagat cgctggagcc aaccggacga taactaccat ttctggccag
    28261 ggtataaacc tgtttatcac catggagcag gtctacacga acgatatctg ccgacatcat
    28321 taattcggta atttgtggcc ttaatgtgct gaagtcattc gacacaaggt gggtatcaat
    28381 cgccgtcgcc accgcctgaa cgcgatgact aaacttatac tgaattgcgt tgtagaaact
    28441 tagcgaacag cccagcaaag tcacaaaaat tgttaacccg gtgagcagcg taacaaaggc
    28501 cgaaaatttc gtcgttaatc tcatccttgt gttaactccg atagtgagga agcgggcata
    28561 ctagcaaatc agatttatct cgcaatttat tgcgcttcat cggctttgct tttccattag
    28621 cgagtatagt cttcagaaat tattttccaa tccatcatgc acatgaggac cacttatgca
    28681 ggcgttactt ttagaacagc aggacggcaa aactctcgca tcggtacaga cacttgacga
    28741 aagtcgcctg ccggagggcg atgtcacggt tgatgttcac tggtcgagcc tgaactataa
    28801 agatgcgctg gcgattaccg gtaagggaaa aatcatccgt aattttccga tgattcctgg
    28861 gatcgatttt gccggaactg tacgcaccag cgaagatccg cgttttcatg ccggtcagga
    28921 ggtgttactc actggctggg gcgttggtga aaaccactgg ggtgggctgg cggagcaggc
    28981 gcgagtgaaa ggtgactggc tggttgccat gccgcaaggg ctggacgcgc gtaaagcaat
    29041 gattatcggt actgccggtt ttaccgccat gctgtgtgtg atggcgctgg aagatgccgg
    29101 tgttcgcccg caggacgggg agattgtcgt gacgggtgcc agtggtggcg tcggcagtac
    29161 cgccgtggcg ctgctgcata agttgggtta tcaggtcgtt gccgtttccg gtcgcgaaag
    29221 tacccatgaa tacctgaaaa gtttaggtgc cagtcgtatt ctccctcgtg atgagtttgc
    29281 cgaatcccgt cctctggaaa aacaagtctg ggctggggca attgacactg ttggcgacaa
    29341 agtgctggca aaagtgctgg cgcaaatgaa ttacggcggc tgcgtggcgg cctgtggtct
    29401 ggcgggtggt tttactctgc caaccacggt catgccattt attctgcgta atgtccgttt
    29461 gcaaggggtg gattcagtaa tgacgccacc agaacgccgc gcacaagcct ggcagcgact
    29521 ggtcgccgat ttaccggaat cattctatac ccaggcggca aaagagatat ctctgtcaga
    29581 ggcaccgaac tttgccgagg ccatcattaa taaccagatc cagggtcgca cgctggtgaa
    29641 ggttaactaa ccatttagca aggaataata agagagggaa ctcattttga catcattatt
    29701 caatcggtta acgggaaaag cggttagccg gacagctttc gtcgaacacc ttggtcagga
    29761 agttgtacaa catcatccaa actggaaagt catgatttcg actgaccaca aattgatgcg
    29821 cattgatact cctctaaaca gccattattg atatgcctcc gtcgcctgtt aggtttatgg
    29881 tgctttgcct gggcgacact acgcttaacc tgttactgat ttctggtccc atggggtgaa
    29941 taatctgatt ttatttgact acaaattaac cactcgaacc tatttaatgc tgagaattgt
    30001 cagtcggttg attttgcgtg ctttagcatt cacatctatc cagacgatgc agtgaaaatt
    30061 gggtaatccc cagcaaccgc tgcgtaatgt cgtctatctt gtcgcgatcc tggcatccct
    30121 acattatttg tggtctgtga agattatctc attgcagccc ctcatcttcg cagggctggc
    30181 tttacagctt ttcaccttac gttataagaa gttccgtcga tgatggcgct aatttcgtga
    30241 attgtgcggc ttgttgcaaa ttacacggtg ttgaaggtta tttacatgtt agctgttgat
    30301 tatcttccct gataagacca gtatttagct gccaattgct acgaaatcgt tataatgtgc
    30361 gacctcgtcc tccctgacac agtttttgcg ctgcggaaaa ggtgacattg gcgcaacgaa
    30421 ggtatatttt gttttttgcc ggaggatagc agcagatcgc tgcacaatgt ccgtcaagtc
    30481 taacattgac actctggggc aaaatagacc ggcgtcccgg cctgctggaa tttatcgcta
    30541 tgcatacagc tgtcggggca tacgctttac agacggcggt gaaacgcctg tcacaatcac
    30601 actaaacaaa gagtacggaa cccactcatg gatattcgta agattaaaaa actgatcgag
    30661 ctggttgaag aatcaggcat ctccgaactg gaaatctccg aaggcgaaga gtcagtacgc
    30721 atcagccgtg cagctcctgc cgcaagtttc cctgtgatgc aacaagctta cgctgcacca
    30781 atgatgcagc agccagctca atctaacgca gccgctccgg cgaccgttcc ttccatggaa
    30841 gcgccagcag cagcggaaat cagtggtcac atcgtacgtt ccccgatggt tggtactttc
    30901 taccgcaccc caagcccgga cgcaaaagcg ttcatcgaag tgggtcagaa agtcaacgtg
    30961 ggcgataccc tgtgcatcgt tgaagccatg aaaatgatga accagatcga agcggacaaa
    31021 tccggtaccg tgaaagcaat tctggtcgaa agtggacaac cggtagaatt tgacgagccg
    31081 ctggtcgtca tcgagtaacg aggcgaacat gctggataaa attgttattg ccaaccgcgg
    31141 cgagattgca ttgcgtattc ttcgtgcctg taaagaactg ggcatcaaga ctgtcgctgt
    31201 gcactccagc gcggatcgcg atctaaaaca cgtattactg gcagatgaaa cggtctgtat
    31261 tggccctgct ccgtcagtaa aaagttatct gaacatcccg gcaatcatca gcgccgctga
    31321 aatcaccggc gcagtagcaa tccatccggg ttacggcttc ctctccgaga acgccaactt
    31381 tgccgagcag gttgaacgct ccggctttat cttcattggc ccgaaagcag aaaccattcg
    31441 cctgatgggc gacaaagtat ccgcaatcgc ggcgatgaaa aaagcgggcg tcccttgcgt
    31501 accgggttct gacggcccgc tgggcgacga tatggataaa aaccgtgcca ttgctaaacg
    31561 cattggttat ccggtgatta tcaaagcctc cggcggcggc ggtggtcgcg gtatgcgcgt
    31621 agtgcgcggc gacgctgaac tggcacaatc catctccatg acccgtgcgg aagcgaaagc
    31681 tgctttcagc aacgatatgg tttacatgga gaaatacctg gaaaatcctc gccacgtcga
    31741 gattcaggta ctggctgacg gtcagggcaa cgctatctat ctggcggaac gtgactgctc
    31801 catgcagcgc cgccaccaga aagtggtcga agaagcacca gcaccgggca ttaccccgga
    31861 actgcgtcgc tacatcggcg aacgttgcgc taaagcgtgt gttgatatcg gctatcgcgg
    31921 tgcaggtact ttcgagttcc tgttcgaaaa cggcgagttc tatttcatcg aaatgaacac
    31981 ccgtattcag gtagaacacc cggttacaga aatgatcacc ggcgttgacc tgatcaaaga
    32041 acagctgcgt atcgctgccg gtcaaccgct gtcgatcaag caagaagaag ttcacgttcg
    32101 cggccatgcg gtggaatgtc gtatcaacgc cgaagatccg aacaccttcc tgccaagccc
    32161 gggcaaaatc acccgtttcc acgcacctgg cggttttggc gtgcgttggg agtctcatat
    32221 ctacgcgggc tacaccgtgc cgccgtacta tgactcaatg atcggtaagc tgatttgcta
    32281 cggtgaaaac cgtgacgtgg cgattgcccg catgaagaat gcgctgcagg agctgatcat
    32341 cgacggtatc aaaaccaacg ttgatctgca gatccgcatc atgaatgacg agaacttcca
    32401 gcatggtggc actaacatcc actatctgga gaaaaaactc ggtcttcagg aaaaataaga
    32461 ctgcaaaagc gtcaaaaggc cggattttcc ggcctttttt attactgggg atcgacaacc
    32521 cccataaggt acaatcctcg ctttcttcac ccatcaggga caaaaaatgg acactcgttt
    32581 tgttcaggcc cataaagagg cgcgctgggc gctggggctg acccttttgt atctggcagt
    32641 ttggttagta gccgcttact tacctggcgt tgcccccggt tttaccggct ttccgcgctg
    32701 gtttgagatg gcctgcatcc tgacgccgct gctgtttatt ggactgtgct gggcgatggt
    32761 gaaatttatc tatcgcgata tcccgctgga ggatgacgat gcagcttgaa gtaattctac
    32821 cgctggtcgc ctatctggtg gtggtgttcg gtatctcggt ttatgcgatg cgtaaacgga
    32881 gcaccggcac cttccttaat gagtatttcc tcggcagccg ctctatgggc ggtattgtgc
    32941 tggcgatgac gctcaccgcg acctatatca gtgccagttc gtttatcggc gggccaggag
    33001 ctgcttataa atacgggctg ggctgggtgt tactggcgat gattcagctt cctgcagtct
    33061 ggctttcact cggtattctc ggcaagaagt ttgcgattct tgcgcgccgc tacaatgcag
    33121 tgacgctgaa cgatatgctg tttgcccgct accagagtcg tcttctggtg tggctggcga
    33181 gtttgagttt gctggttgcg ttcgttggtg cgatgaccgt gcagtttatc ggcggtgcgc
    33241 gcctgctgga aaccgcggcg ggtattcctt atgaaaccgg gctgctgatt ttcggtatca
    33301 gcattgcgtt gtataccgcc tttggcggct ttcgcgccag cgtgctgaac gacaccatgc
    33361 aagggctggt gatgctgatt ggcaccgttg tgctgcttat tggcgtagta catgccgctg
    33421 gcggcttaag caacgcagta cagaccttgc aaaccatcga tccgcaactg gttacgccac
    33481 aaggcgcgga cgatattctg tcgcctgcct ttatgacgtc gttctggata ctggtgtgct
    33541 ttggcgttat aggcctgccg catactgcgg tgcgctgtat ctcttataaa gacagcaaag
    33601 cagtacaccg ggggatcatc atcggtacga ttgtagtcgc aatcctgatg ttcggtatgc
    33661 acctggccgg agcgttaggt cgtgcagtga tccccgatct caccgtaccg gatctggtga
    33721 tcccgacgtt aatggtaaaa gtgctgccac cgtttgctgc cgggatcttt ctggctgcac
    33781 ctatggctgc gatcatgtcg acaattaacg cccaattgct acaaagttcc gctacgatca
    33841 ttaaagatct ctatctgaat atccgtccgg atcaaatgca aaacgagacg cgtctgaagc
    33901 ggatgtcggc ggtaattacg ttagttctcg gcgcgttgct gctgcttgcc gcctggaagc
    33961 cgccagaaat gatcatctgg ctgaatttgc tggccttcgg tgggctggaa gccgttttcc
    34021 tgtggccgct ggtgctgggt ctttactggg aacgcgccaa cgccaaaggc gcgctaagtg
    34081 cgatgatcgt tggcggcgtg ctgtatgccg tactcgcgac gctgaatatt cagtacctgg
    34141 gcttccaccc tatcgtgccc tcgttactac taagtttgct ggctttcctg gtcggaaacc
    34201 gtttcggtac atccgtcccg caagctaccg ttttgactac tgataaataa agagttttgc
    34261 catgccttgg atccaactga aactgaacac caccggcgcg aacgcggaag atcttagcga
    34321 tgcgctgatg gaagcgggtg ccgtttctat cacttttcag gatacccacg atacgccagt
    34381 atttgagccg ctgccgggcg aaacgcgcct gtggggcgac accgatgtga ttggtctgtt
    34441 cgacgctgaa accgatatga acgacgtggt ggcgattctg gaaaaccatc cgctgctcgg
    34501 cgcagacttc gcgcataaaa tcgaacaact agaagataaa gactgggagc gcgaatggat
    34561 ggataatttc cacccgatgc gctttggtga acgactgtgg atctgcccta gctggcgtga
    34621 tgtgccggac gaaaacgccg tcaacgtgat gttagatcca gggctggcgt ttggtacggg
    34681 tacccatcca accacctctc tgtgcctgca atggctcgac agcctcgatt taaccggtaa
    34741 aacggtgatc gactttggct gcggttccgg cattctggct atcgcggcgc tgaaactggg
    34801 tgcagcaaaa gccattggta ttgatatcga tccgcaggcg attcaggcca gccgcgacaa
    34861 cgccgaacgt aatggtgttt ctgagcgtct ggaactctac ttaccgaaag atcagccaga
    34921 agcgatgaaa gccgacgtgg tggtcgctaa catccttgca ggcccattac gtgaactggc
    34981 accgttaatc agcgtcctgc cggtttcagg cggtttgctg ggcctttccg gtattctggc
    35041 aagccaggca gagagcgttt gtgaagctta tgccgacagc ttcgcactgg acccggtcgt
    35101 ggaaaaagaa gagtggtgcc gtattaccgg tcgtaagaat taaccttcgc atcgccgtag
    35161 ggtgacgcgg ggcgaagtgc gagcaagctc acaaaaggca cgtaaatttg ccgattattt
    35221 acgcaaattt gcgtgccaaa attttcattc ataaagaaaa attgagaact ta
//
LOCUS       DAPGOF010000028        35164 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.028, whole genome shotgun sequence.
ACCESSION   DAPGOF010000028 DAPGOF010000000
VERSION     DAPGOF010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35164
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.028"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(37..402)
                     /locus_tag="RHV13_001221"
     CDS             complement(37..402)
                     /locus_tag="RHV13_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDV1240775.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            complement(685..1671)
                     /gene="yqjG"
                     /locus_tag="RHV13_001222"
     CDS             complement(685..1671)
                     /gene="yqjG"
                     /locus_tag="RHV13_001222"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HDV1240776.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVEKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            complement(1741..2133)
                     /locus_tag="RHV13_001223"
     CDS             complement(1741..2133)
                     /locus_tag="RHV13_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDV1240777.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITGYAGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            complement(2319..2618)
                     /locus_tag="RHV13_001224"
     CDS             complement(2319..2618)
                     /locus_tag="RHV13_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HDV1240778.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            complement(2608..3012)
                     /locus_tag="RHV13_001225"
     CDS             complement(2608..3012)
                     /locus_tag="RHV13_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDV1240779.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            complement(3015..3320)
                     /locus_tag="RHV13_001226"
     CDS             complement(3015..3320)
                     /locus_tag="RHV13_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HDV1240780.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            complement(3358..3726)
                     /locus_tag="RHV13_001227"
     CDS             complement(3358..3726)
                     /locus_tag="RHV13_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HDV1240781.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            complement(3873..4256)
                     /gene="mzrA"
                     /locus_tag="RHV13_001228"
     CDS             complement(3873..4256)
                     /gene="mzrA"
                     /locus_tag="RHV13_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HDV1240782.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPQGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            complement(4260..4922)
                     /locus_tag="RHV13_001229"
     CDS             complement(4260..4922)
                     /locus_tag="RHV13_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDV1240783.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            complement(5268..6044)
                     /gene="exuR"
                     /locus_tag="RHV13_001230"
     CDS             complement(5268..6044)
                     /gene="exuR"
                     /locus_tag="RHV13_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HDV1240784.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            complement(6174..7472)
                     /gene="exuT"
                     /locus_tag="RHV13_001231"
     CDS             complement(6174..7472)
                     /gene="exuT"
                     /locus_tag="RHV13_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HDV1240785.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            7955..9367
                     /gene="uxaC"
                     /locus_tag="RHV13_001232"
     CDS             7955..9367
                     /gene="uxaC"
                     /locus_tag="RHV13_001232"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDV1240786.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            9382..10869
                     /gene="uxaA"
                     /locus_tag="RHV13_001233"
     CDS             9382..10869
                     /gene="uxaA"
                     /locus_tag="RHV13_001233"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HDV1240787.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            10952..11503
                     /locus_tag="RHV13_001234"
     CDS             10952..11503
                     /locus_tag="RHV13_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HDV1240788.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(11508..12752)
                     /gene="sstT"
                     /locus_tag="RHV13_001235"
     CDS             complement(11508..12752)
                     /gene="sstT"
                     /locus_tag="RHV13_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HDV1240789.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWFSKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(13151..14116)
                     /gene="alx"
                     /locus_tag="RHV13_001236"
     CDS             complement(13151..14116)
                     /gene="alx"
                     /locus_tag="RHV13_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HDV1240790.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRVG"
     gene            complement(14400..15386)
                     /locus_tag="RHV13_001237"
     CDS             complement(14400..15386)
                     /locus_tag="RHV13_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDV1240791.1"
                     /translation="MIRFAVIGTNWISRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQAQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARDNQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(15465..16157)
                     /locus_tag="RHV13_001238"
     CDS             complement(15465..16157)
                     /locus_tag="RHV13_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HDV1240792.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWSDVNVVPARNVGLLLGARPGNRYFTRRIDTAAELYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYDIDAIGFNAPDLNMKHGLYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(16234..16737)
                     /locus_tag="RHV13_001239"
     CDS             complement(16234..16737)
                     /locus_tag="RHV13_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HDV1240793.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            16822..17958
                     /gene="rlmG"
                     /locus_tag="RHV13_001240"
     CDS             16822..17958
                     /gene="rlmG"
                     /locus_tag="RHV13_001240"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HDV1240794.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            complement(18069..20087)
                     /gene="fadH"
                     /locus_tag="RHV13_001241"
     CDS             complement(18069..20087)
                     /gene="fadH"
                     /locus_tag="RHV13_001241"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HDV1240795.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYHNRMRFAVEVVSAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMLILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRTPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIAEFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPSVSYQKIDDDGLHVVINGEPQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(20610..22961)
                     /gene="ygjK"
                     /locus_tag="RHV13_001242"
     CDS             complement(20610..22961)
                     /gene="ygjK"
                     /locus_tag="RHV13_001242"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDV1240796.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGRVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTDQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKFNTITPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKTWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLNKYVASGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPATQ
                     YFYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(22978..24048)
                     /gene="ygjJ"
                     /locus_tag="RHV13_001243"
     CDS             complement(22978..24048)
                     /gene="ygjJ"
                     /locus_tag="RHV13_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HDV1240797.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTSAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASNDPSDQIFLMQYFYW"
     gene            complement(24182..25615)
                     /locus_tag="RHV13_001244"
     CDS             complement(24182..25615)
                     /locus_tag="RHV13_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDV1240798.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(25678..26127)
                     /locus_tag="RHV13_001245"
     CDS             complement(25678..26127)
                     /locus_tag="RHV13_001245"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HDV1240799.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDCEYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(26124..29216)
                     /gene="ebgA"
                     /locus_tag="RHV13_001246"
     CDS             complement(26124..29216)
                     /gene="ebgA"
                     /locus_tag="RHV13_001246"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HDV1240800.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKQLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSTIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDADGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNIMVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNLQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            complement(29400..30383)
                     /gene="ebgR"
                     /locus_tag="RHV13_001247"
     CDS             complement(29400..30383)
                     /gene="ebgR"
                     /locus_tag="RHV13_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HDV1240801.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            30602..30934
                     /locus_tag="RHV13_001248"
     CDS             30602..30934
                     /locus_tag="RHV13_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HDV1240802.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            complement(30976..32355)
                     /gene="ygjG"
                     /locus_tag="RHV13_001249"
     CDS             complement(30976..32355)
                     /gene="ygjG"
                     /locus_tag="RHV13_001249"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HDV1240803.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            32773..34293
                     /gene="aer"
                     /locus_tag="RHV13_001250"
     CDS             32773..34293
                     /gene="aer"
                     /locus_tag="RHV13_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HDV1240804.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGYT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTGKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYILCALVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(34447..35070)
                     /gene="nfeR"
                     /locus_tag="RHV13_001251"
                     /gene_synonym="yqjI"
     CDS             complement(34447..35070)
                     /gene="nfeR"
                     /locus_tag="RHV13_001251"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HDV1240805.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDRAAFDITQLD"
BASE COUNT         8522 a         9196 c         9200 g         8246 t
ORIGIN      
        1 gccggatgaa tcagacatcc ggcgagcaga tttgttttaa ggttccagtt ttggatcagg
       61 tccaaagcgg ttttcgcctg gcgtacccgc ctggcagtta aagacgatga taataagcca
      121 gccgataaac gggattaaga acagcagtgc ccaccacgcc gagcggtcgg tgtcgtgcag
      181 gcggcggaac tgaaccgccc accacggtaa aaacaccaga ataccgtaga ttgtcgtcag
      241 gatgccttcg ccaccagcgc gttgccagcc taacatttta tccagcagcc ccagcacgaa
      301 cgtaaagatg atgttgacca gaataaacat ccagtactct ttgcgccgcg cacgaccccg
      361 gaaaccgaca taatttttta gtactttcag ataccagtcc atttttgcct caattttgct
      421 caatcacttg tttttaaagc agcaaattaa gaatagacgt gaatgaaatg gcagtaaata
      481 ttatcgcgat gaattaataa ggagctgtga aggataaggg caagcgcaaa atagccagat
      541 gcgctacgct tatctggcct acgcggcctt tgcaacatat tgaatataaa agttttgtag
      601 gtttgataag gcgttcatgc cgcatccgac atgaacaaca tgcacttagc taattatcag
      661 aggcaccttg taaggtgcct ttttttaacc gaagcgaaca tctcgtccat gcggttcatc
      721 gagatcctgc cacggaccaa ttgaaataat ccccgtaggg ttgatggtct tatgactgcg
      781 gaagtaatga ttacggatat gatcgaaatt gactgtttcg gcaattcccg gcatctggta
      841 gatatcacgc aggaagccat acagattaag gtaatcgctg atgcggtgct tatcacactt
      901 gaagtgggtc acatacactg gatcaaaacg gaccagcgtg gtccacaggc gaatatcggc
      961 ttcggttagc tggttgccgg tcaggtaacg atgctgacct aaaatctgtt ccagtcgcgc
     1021 cagcgattca aacactttct ccaccgcttc gtcgtaggct tgctggctgg tggcaaaacc
     1081 agctttatac acgccgttgt taacggtgtc ataaatccag ccgttaagtt cgtcaatttt
     1141 cgtttgcagg gctggtgggt agtaatcacc agctttcgcg cccagcgcat caaacgcggt
     1201 attaaacatg cggatgattt ctgctgattc gttgctgacg atggtgtggt tctttttgtc
     1261 ccacagcacg ggaacagtaa ctcgtccgct gtagtgtgga tcggcgtgga gataaagctg
     1321 atacaaaaat tcatgttgat agagcgtgtc tccggttgct cccggaaaac tgtcatcaaa
     1381 ggtccagccg ttttccagca tcagcgggtt cactacggaa acggaaataa acggttccag
     1441 tcctttgagt ttgcgcatga tcagcgtgcg gtgcgcccac gggcaggcga gtgaaacata
     1501 gagatgataa cgatctttct ctgcgataaa accgcctgtg ccagtggggc caggagcgcc
     1561 atcggcagtg agccagttac gaaatgcgga agctgaacgt tgaaatttac cgccggtaga
     1621 tttggtatcg taccaggtgt catgccagac gccgtcaatc agttgaccca tttttctctc
     1681 ctccaatagc aaaaacgagg agcttcctcc tcgtttttta tcgttaagta tagcgcgtgc
     1741 ttaccacttt ttattcagca ggcggtcgat gctatacgcg cccggaccgg taattgccag
     1801 cagcaggaat ccgccagaaa ttgtcaggtt tttcatgaac atcagcgagt tgacgccttc
     1861 agcaaagttg ctgtgaaata aaaatgccgt cagcagcgta aagcccgcag taaacagggc
     1921 tgtggtgcga gtcaggaaac cgaacaggat tgccagacca ccaccaaact caagcagaat
     1981 caccagcggc agcataaaac ccgggacgcc catcgcttcc atatattgtt gggtacccgc
     2041 gtaaccagta atttttcccc agcctgcggt aataaacaga atcggcatta aaatgcgcgc
     2101 taccagtaca ccaacatctt ctaatttttt catcatactc tccactgcgc cactcaggct
     2161 gctgattatt tttttgtgca agggcgcggt attagcgtcg ttgctgtcga tggagagaat
     2221 cataaacgtg gtgaatgatg attgttagca aggaaaactg tcaaaaatct tcaaaaaatt
     2281 tgatgggtaa ggccggaatg gctccggcca gagggaagtt aaccgcgaag ctgttgctgc
     2341 ttgagggtcg ttttaaccag acgccaggcg ctccatacgc caaaaccgcg tctggcccag
     2401 cggaccagca tattaggatg gcgaatcgtc cagatcgcca tcacgctact gccaaccagc
     2461 gcccaggagc gcagacttag cagcatattc cagcgacgat cgtaagcgcc tgttgcctcc
     2521 agccattcac gacgactggc agaaagatcc agccgttgtt gctggatctg gctaagcagt
     2581 tgcgccttac gtcgttcacg ttcgacttta ctgctcacgg gactcctcct cgagcagctg
     2641 ccgatcgttt gccagctcat ggcgtgtatg gcgcagcaag gtggatttac gcgatttacg
     2701 taacgtccag ataccgccaa tcagtgccag tagcagcaac accacggtgg tggcaatcat
     2761 cgcattcagg cgatattgcg gatcaaccgc ccaaataatt agcaccatca ggctcataag
     2821 accaaatgca gcgaaaagca tcgtcaggcc cagcatcagt aaaagttgaa agagattagc
     2881 tttttcctct tccagctcca ccaccgccag ccgcagacgt gtttccacca tttcaaccat
     2941 gatggaaaca attcgctgcc cgatgcccag aacgctttta ccgggccctt gcgcgtgatg
     3001 agtgtccgcc ataattaacg acgcgacagc agaacgccga gcactacacc gattgcagcg
     3061 ccaatgccca cgccggtcca cggattttca cgcacatact catcagcacg cgccgccgcg
     3121 acacgggttt gtttggcgat ggcatcaccg gtttcaccca ggcgataacg gctctgtttc
     3181 agtgcctgct ccgctttgct acgaatctta ctcaactctt ctttcgactt ctcgccagat
     3241 gagctaagca cctcttccag cgtatcggaa agggatttca actcagcacg cagatgttcc
     3301 gtagtgtgtt ctttcgacat agttttctcc aggtgagtaa gtagtgacta ttgtgagtta
     3361 gtagtcgcgc gcttccagct ttttcagctc ttcctgcgct tctgccagtt tccgttcgcg
     3421 tttggcaatc ttatcggcat cgcctttttg cttcgcctcg gctaaatctt gctggcgttc
     3481 cgccacctca tctttctgct ttgcgatttt cttgtgatga tcggcacgca gctggctatc
     3541 tgaacagttg gcccggactt cactcagggc tttattcaga ccgtcaatac gattctggtt
     3601 ttggtgtttt tcggcatagc tgatctcctt aaggatattt tgctcctttt cctgacacag
     3661 ggtagtggca taactaccgg cactaagagc aaagagagaa acagctaaag cgatgcggta
     3721 tttcattctt gaaccttcca ttgtgattcg gacctattac aaaggaacga ttgtccagac
     3781 aacgaagcgg tagtgttaac caccgcctca ttaatcatag acagttacgg tgaaatcacc
     3841 aaagtgagtg atatgtttcg gataacagga agttatccga agcgatgaga gttatcccgt
     3901 aaccgggtca gccactgcat agcctgactg ttattgtcct gctgcgcaat gatgtagcct
     3961 tggggcaatg ttctgtcgag gaccgctttt gcagcggcgc tctggtcgct ggagtcaaag
     4021 gtaattaaca gagtgtcgtt tttgggggtg atacttttga aaggaatgcc atgagcgtca
     4081 aggtgatgcc agattgaaaa accgtctggc atcgttgtgc cttgatgaac ggcacgaatc
     4141 gccagcgtag actcttgctg gcgaaccgcg gaccaggcca gcaacagcgt gccgaccaga
     4201 agtaaaacag cgccggacca ggctagctgg cgaagcgaca tgcgaggtat ttgcatccct
     4261 tacccccgat ttccatattt ctttttccat aacacgacca gagaacctgc caggccaaac
     4321 accagcagca ccaccgggag cagcatcagg catgacatca gctggtcctc gtactttaaa
     4381 aataccggcg ttttgccgag catgtaaccc agagttgtca ggatcaatac ccacagcaga
     4441 ccgctcatcc agttgaaaaa ctgaaagcgc gcgttattca gccctgataa cccggcaatc
     4501 gtcggcagca gtgttctgac aaacgcaata aagcgaccaa ttaacagcgc cgataaaccg
     4561 tgtttatgaa aaagatggtg tgcgcgttga tgataatgcg cgggtaaatg agatagccag
     4621 ttttgtacgg tgcgggtatt gcccagccat cgcccctgaa tatagctgac ccagcagccg
     4681 aggctggcgg caacggtcag cagcagaatc gtttgcggat agcccatcgc gcctttcgca
     4741 atcaacacgc cgaccaatac cagtaaactg tcgcccggta aaaaggccgc cggaagcaag
     4801 ccgttttcaa ggaacaaaat tacaaacaag acaaaataca acatgccaat catcgatgga
     4861 ttggccaggg tttcaaaatc ctgcgcccac agggcttgca gcaattgggt caaaagttcc
     4921 attcattatt cctggtgaat cggttaacgc cacgctggtt ttaaaaagat gcactaccgc
     4981 aatgttattg ctgttttggt atgttctgcg ggtttgatac atcaacacag cctgaactat
     5041 tccctgttaa atcgtgagat aagaaagcat tcaaaaacat aaattcaaat tgaatctaat
     5101 tgtaacaaag gtcaacgttg ttcgttatca aaattacatg tccgtggagt ctgattttgc
     5161 tgaatgcagg ggagggtggc gagaggattt acacaggctg acactttata cttttcgctt
     5221 accgacgaga caaacaggcg cgtcgcagcg cctgtcggac aggtatttca tttactgcca
     5281 ctggtggccg tatcgagatg cactaccgga ttttcggcga acagataacg gtcggcattg
     5341 aactcgaagt cgtcgctggt ttcgttaaat aacatgatct tggtgttttc caggtgctgc
     5401 cacattgcca gcttagcagc atgaggatct ttgcgaatca gcgccttgag gatttgatcg
     5461 tggtcatcac accagttatc gacggtacgg gaatcaatgt gttcgtgcag ttttttccag
     5521 tacgggttat ggctacgctg ggtccacatt ttttcaacga tagccgccag ggcggagttc
     5581 tgcgtcgcca gggctacctg aatatggaac tgcaaatccc attcggaatc acggaagcat
     5641 tgttcgccgc gcgcctgctc ctggatggcc atcaatttca tgatgtcctg tttcgttacc
     5701 tgagttgctg cgaactcggc aatattactt tcgatgagtt ggcgagcctg gagtaactca
     5761 aacggaccgt aattggcgaa ttccatatta ttgtccgcag cctgctgatg gcgcggctgg
     5821 ttggaaacca cgtgaatacc tgaaccttta cgcacttcga catagccttc aacttccagc
     5881 atgatgatgg cttcacgaac taccgtacgg ctgacgttct tttcatcggc aataaagcgc
     5941 tcggcaggca gtttatcacc caccagatag acgccctgtt cgatgcgctc tttcaggtca
     6001 gcggcaagtt gttgatacaa acggcgtggt tcagtgattt ccatatgcgc tccaggcatg
     6061 atacggcaga ctatatttgt tataccactt ttgcgagttt agctctactc gcagtgatga
     6121 aaaagccgcc tgagaaggcg gctttgaagg gaaactaaaa cttacacgaa gcgttaatgt
     6181 tgcggtgcgg gatcgttatg tgtttcctgc gccacctcaa tcgccggttt gttctgcaat
     6241 acggtccaga taaccagcgc acccagcagg tcaaatactg ccagaactgc gaacagcggg
     6301 ctgaagccga tggtgtcagc cagtgcaccg acaaccagcg caaacagcgt acttgccagc
     6361 catgcggaca tcccggttaa accgttcgcc gtcgccactt cgttacgacc gaacacgtca
     6421 gaagagagcg taatcagcgc gccagacagt gcctggtgag caaaaccacc gatacacagc
     6481 agcataattg cgacatacgg gttggtgaac aaaccgatca tacccgggcc aatcatcagc
     6541 actgcaccca gcgttacgac catcttacgg gaaacgatca ggttcacacc aaaccaacgc
     6601 tggaacagcg gcggcaggta accaccgagg atacaaccga ggtcagcaaa cagcatcggc
     6661 atccaggcga acatcgcgat ctctttcagg ttaaagccgt aaactttaaa catgaacagc
     6721 gggatccacg cgttaaaagt accccaggcc ggttctgcca ggaaacgcgg cagcgcgata
     6781 ccccagaact gacggttacg caggatctga ccaacggaca ttttcttcgc cgtgctcacc
     6841 tggtgctggg cttcctgacc attaataata tagtcgcgtt cttcatcggt cagatgcttc
     6901 tggtcgcgcg gatgtttata gaaaatcagc catgccattg cccagataaa gctcaatgca
     6961 ccggagatga taaatgccat ctgccagctg tgcattacga ttgcccatac caccagcggc
     7021 ggcgcaatca tcgcaccaat cgaagaacct acgttaaagt agcctactgc gatggaacgc
     7081 tctttcgccg ggaaccattc ggagctggct ttcagacccg ccgggatcat cgctgcttcc
     7141 gcggcaccga ccgcaccacg agcaacagcc aggccacccc agctacctgc cagcgcggtt
     7201 gcaccacaga acacggccca cagcacagca aacattgcat aaccgatttt cgtacccagc
     7261 acatccagta cataacctgc taccggttgc atgaccgtat aagcagcaga ataggctgcg
     7321 atgatatagg aatactgttg ggtggagatg tttaactctt ccatcagagt tggcgcagct
     7381 gccgccacag tgttacgcgt caggtaacca agcacggtgc cgagcgtcac cagtgcgatc
     7441 atataccaac gtaacccttt aattttacgc atgtaaaacc tcatcgtttt gttatatccg
     7501 caggaagcgc ggccatcatc ccgccagagg ctgaagatgt tagttacgac cgggtaaccg
     7561 aaaaaaaacc ggcacgcccc gaacgttgcc atgaaaattt cgtgctaatt cggcttccgt
     7621 accggttaac ccgattcatc taacaaatga atgaatcggc aatttgcatt ccgtcgactt
     7681 atgatttgcg acggcagaaa gataacttgt catacaactt taaaaggtga gagccatcac
     7741 aaatgtgcga atatttgtag ggacattacc tgacgacagc gagaccagta ctggcgcggc
     7801 ctgcagcgag atttaccact ttgagagtaa tttttttaac tacgtttatt gatctaactc
     7861 acgaaaatat cttcggactc tggaaattgg tgtgataact ttgtcagcat cgcaccataa
     7921 gcaagctagc tcactcgttg agaggaagac gaaaatgact ccgtttatga ctgaagattt
     7981 cctgttagat accgaatttg cccgccgtct gtatcacgac tacgcaaaag accagccgat
     8041 tttcgattac cattgccatt tgccgccgca gcagattgcg gaagactatc gttttaaaaa
     8101 cctgtatgac atctggttga agggcgatca ctacaaatgg cgcgctatgc gtaccaacgg
     8161 tgtggctgag cgtctgtgta ctggtgatgc gtctgatcgt gaaaaatttg acgcctgggc
     8221 ggcgactgtt ccgcacacta tcggcaaccc gttataccac tggacgcacc tcgaactgcg
     8281 tcgtccgttc ggtatcactg gcaaattgct ttctccgtca actgccgatg aaatctggaa
     8341 cgaatgtaac gaactgctgg cgcaggataa cttctccgcg cgcggcatca tgcagcagat
     8401 gaacgtgaaa atggtcggca ccaccgatga cccgatcgat tctctggagc atcacgcaga
     8461 gatcgccaaa gacggctctt tcaccatcaa agtgctgccg agctggcgtc cggacaaagc
     8521 ctttaacatc gaacaggcga cctttaacga ctacatggcg aagctgggcg aagtttccga
     8581 taccgacatt cgccgctttg ctgacctgca aactgccttg accaaacgtc tggatcactt
     8641 cgccgctcac ggctgtaaag tgtctgacca tgcgctggac gtggtgatgt ttgccgaagc
     8701 gaacgaagcg gaactggaca gcattctggc gcgccgtctg gctggcgaaa ccctgagcga
     8761 gcacgaagtg gcacagttca aaactgcggt actggtgttc cttggtgccg aatatgcacg
     8821 tcgcggctgg gtacagcagt accacattgg cgcgctgcgt aataacaacc tgcgtcagtt
     8881 caaactgctg gggccggatg taggctttga ctccatcaac gaccgtccga tggcggaaga
     8941 gctgtctaag ctgctgagca agcagaacga agaaaacctg ctgccgaaaa ccattctcta
     9001 ctgcctgaac ccgcgcgata acgaagtgct gggcaccatg atcggtaact tccagggcga
     9061 aggtatgccg ggcaaaatgc agttcggttc cggctggtgg tttaacgatc agaaagacgg
     9121 tatggaacgt cagatgaccc aactggcgca gctcggtctg ttgagccgct ttgtcggtat
     9181 gctgactgac agccgtagct tcctgtcata cacccgtcac gaatacttcc gccgcattct
     9241 gtgccagatg atcggtcgct gggtggaagc gggcgaagca ccagcggaca tcaacctgct
     9301 gggcgagatg gtgaaaaata tttgctttaa caatgcgcgt gactacttcg ccattgaact
     9361 gaactaaggt ctggggttga tatgcaatac atcaagatcc atgcgctgga taacgtcgcg
     9421 gtcgctttag cagatttggc ggaaggcaca gaagtcagtg tcgataacca gactgttacg
     9481 ctgcgccagg atgttgctcg tggacataaa tttgcgttaa cggatatcgc aaaaggggcc
     9541 aacgtcatca aatatggcct gccgattggt tatgcattgg cggatattgc ggcgggggaa
     9601 cacgttcacg cccacaatac gcgcacgaat ctgagcgatc tggatcagta tcgctatcaa
     9661 cctgattttc aggatctgcc tgcgcaagcg gcagatcgtg aagtgcagat ctatcgtcgc
     9721 gctaacggcg atgtcggggt gcgtaacgag ctgtggatcc tgccaaccgt gggctgtgtc
     9781 aacggcatcg cgcggcagat ccagaaccgt tttctgaaag agaccaataa cgctgaaggt
     9841 accgacggcg tgttcctctt cagccacacc tacggctgct cacagctggg cgacgatcac
     9901 attaataccc gcaccatgct gcaaaacatg gtgcgccacc cgaacgcagg cgcagtgctg
     9961 gtgattggcc tgggctgtga aaacaaccag gttgccgcat tccgtgaaac gctgggcgat
    10021 atcgatcctg aacgcgttca tttcatgatc tgccaacagc aggatgatga gatcgaggcc
    10081 gggatcgagc atttgcatca gttgtataac gtgatgcgca acgataaacg cgagccaggc
    10141 aaactcagcg aactgaagtt tggtctggag tgcggtggtt ctgacggtct ttctggtatt
    10201 actgctaacc cgatgctggg gcgtttctct gactacgtga ttgctaacgg cggtactacc
    10261 gtactgaccg aagtgccgga gatgtttggc gcagagcagt tgctgatgga ccattgccgc
    10321 gacgaagcaa cgtttgaaaa actggtcact atggtcaacg acttcaaaca gtactttatt
    10381 gcccatgacc agccgatcta tgaaaaccca tcgccgggga acaaagcggg cggtatcacc
    10441 acgctggaag acaaatcact tggctgtacc cagaaagcgg gttccagcgt cgtggttgac
    10501 gtgctgcgtt acggcgagcg tctgaaaacg ccagggctga acttgttaag tgcgccgggt
    10561 aacgatgccg tagcgaccag cgccctggcg ggtgcgggct gccatatggt gctgttcagt
    10621 actggtcgtg gcacgccgta tggtggattt gtgccgacgg tgaaaattgc caccaacagt
    10681 gaactggcgg cgaagaaaaa acactggatc gactttgacg cgggtcagct gatccacggt
    10741 aaagcgatgc cgcagttgct ggaagaattt atcgacacca tcgttgagtt tgccaacggt
    10801 aagcaaacct gtaacgagcg taacgacttc cgcgaactgg cgatcttcaa aagcggcgta
    10861 acgctataaa gtcgaaatga gttgttaata aaaaacggcg tttcataatg tgaagcgccg
    10921 tttgcttttc catacctgct aaggatttat catgaccgcg tattggctgg cccagggcgt
    10981 gggtgtcatc gcctttctga ttggtatcac aacatttttc aatcgtgacg aacgtcgctt
    11041 caaaaagcag ctttcggtct atagcgccgt tattggcgta catttttttc ttctgggcac
    11101 ctatcccgct ggtgccagtg ccatccttaa tgccattcgt acattgatta ccttacgcac
    11161 gcgcagctta tgggtaatgg cgatttttat tgtgctgact ggcggaattg gcctcgcgaa
    11221 gttccatcat cctgtcgaac tattgccggt tatcggtacg attgtcagta cctgggcgct
    11281 gttccgctgt aaagggctca ccatgcgctg cgtaatgtgg ttttcaacgt gttgctgggt
    11341 gattcacaac ttctgggcgg ggtcgatagg cggcacgatg attgagggta gttttctgct
    11401 tatgaatggc ctgaatatca tccgtttctg gcggatgcag aaaaggggaa ttgatccgtt
    11461 taaagtagag aaaacccctc ccgccataga cgaaaggggt taaacaatta attacgcagg
    11521 gcgctatttg ccagacgatc gtcttctgcc tggcaagctg ccgcagtgaa tagcacgtca
    11581 gttgaagagt tcagcgcggt ttcgcaagag tcctgcaata cgccgatgat aaagccgacg
    11641 gcaaccacct gcatggcgat atcgttcgaa ataccgaaca tattacaggc cagtgggatc
    11701 agcagcagag acccccccgc cacgccggat gcgccacagg cacacagaga agccactacg
    11761 ctcaacagca gcgctgtggg cagatcgacc gggataccca gcgtattaac cgcagccagc
    11821 gtcaacacgg taatggtgat tgctgcgccc gccatattga tggtggcacc cagcggaata
    11881 gaaacggaat aggtatcgcg atccagattc agcttttcac acagcgccat attcaccgga
    11941 atgttcgccg cagagctgcg ggtgaagaag gcatacacgc cgctttcgcg caggcacagc
    12001 agcaccagcg ggaacgggtt acgacgaatt ttccaccaca ccagcaatgg gttaaccacc
    12061 agcgccacca gtaacataca gccaaccagc acgaccagca gttgcgcgta gccccacagt
    12121 gtggagaaac cggtggttgc cagggtagaa gaaaccagcc caaaaatacc gatcggtgcg
    12181 aagcgaatga ccagtttcac cataaaggtg acggcattcg acatatcgtt gaccaggttt
    12241 ttggtggttt cgttaccgtg acgcagtgcg aaaccgaggc caattgccca caccaggatc
    12301 ccgatgtagt tacctttcag cagcgcgtca atggggttgg aaaccatgct cattactagt
    12361 ccgcgcatca cttcgacaat gcctgacggc ggcgaaatat cacccgcgct actggacaag
    12421 tgcagggtag aagggaaggc aaagctgaag actactgcgg ccagagctgc agagaaggtg
    12481 cccagcagat agaggaacaa aataggacgg atattggttt tctgcccgtg ctggtggtta
    12541 gcaatagatg ccattaccag catcagcacc aggatggggg caacagcttt cagtgcgccg
    12601 acgaacaaag tacctaacag accaacagct tccgccgcgg gttttgagaa ccatgccaga
    12661 agaatcccca gaacaaggcc gaccaggatt tgttttacca ggctgccatg agccagacgc
    12721 cggaatagcc ccggtgaacg ttgcgtagtc atttttcgat cctttcattg tgttgtcgca
    12781 catccctggt gcgttctgtc gacgcatctt tcaggatgta acaaagtgtt tgcaaggtcg
    12841 agtataagga aagttacagc ttcgggaagc gaaaaatgct ggattttaaa gggaggtcat
    12901 gttttgcaac ttaatggtta catttatttg acataaaaat aaagccaacg tgcggcttcc
    12961 agactgagca ctacgtcaaa aaaggactcg gtcagtcccc tcgccccgtt tggggagagg
    13021 gttagggtga ggggaacagg ccagcatcgg tgcgaaaaat taccctcacc ccgaccctct
    13081 ccctggaagg gagagggaga aaacggtgct catgttgatg acagggtaca cggcttaggc
    13141 agattaaaaa ttatccaacc cgctgcttat catgccgata attcacccag gcgttgataa
    13201 taaacgtcat caccaggatg ccaaacacca cgcccagcga gacggcgatc ggaatatggt
    13261 agaagtcgac aatcagcatc ttgataccga taaacaccag aatcaccgcc aggccatatt
    13321 tgagcatcga gaaacgctct gctacgcccg ccagcaggaa atacatcgca cgcaggccga
    13381 ggatcgcaaa caggtttgag gtcagcacaa tgaacgggtc agtggtcacg gcgaagatag
    13441 ccggaatgct atccacggca aaaatcacgt cgctcaattc caccagaatc agtaccagca
    13501 tcagcggtgt ggcgtacagc aatccattct tacgcacaaa gaaatgctca ttgtcgatgg
    13561 tgtcggtcat gcgcaaatga ccgcgtagcc agcgcaccag cggcttgtcg ccaatgcctg
    13621 attcatcttc atgggcgagc gccatcttca cgccggtaaa cagcaggaag gcaccgaaga
    13681 tatacaggat ccagtcgaac tgcgaaatca gccagctgcc tgtgaagatc atgatggtac
    13741 gcagaacaat cgccccgagc acgccataca ccagcacgcg acgttgtaat gccgccggaa
    13801 cggagaaata gctgaacaac atcagccaga caaagacgtt atcgaccgcc agcgatttct
    13861 caatcagata acccgtgaga aaggccagtg cctgtggatc ggcaacggcg cgaccttcgg
    13921 tttgcaccag ataccaccag aaagcggcgt taaacagtaa cgacagcgtc acccagacca
    13981 gcgaccaggc cgcagcctgt ttcatggtca tggcatgtgc cccacgacgc ccctgcaaca
    14041 acaggtcgat agccagcata atggcgacaa caacagcgaa tccgccccat agcaacggcg
    14101 tgccgacagt attcatagaa gttccttaca cataaaaaaa acggccaacg tcggaagacg
    14161 tcagccgctg ctttttatgc atagacctcg ccttccggca aggtctcact tacaacaaaa
    14221 aaggaatacg tctgcgtatt cctttcgggt tgcccggtga ccggatgtgg tttttcacac
    14281 atcgtaatga cgatcgaccg gcaatgaagt tactcccctt tgcaggtaac aaagtatgac
    14341 agaccattca gtcagtcaat ggtctgttac atacctttta cactgtttta ctttggcaat
    14401 tatagtttta cgctatctgc cggaaaaatc accccagtct ggcggcggat ctcggtcagc
    14461 agtttggcgg tgatgcgact gaccgccagc cctggatgat ccaccagatg ctcatccacc
    14521 agggtagcga acagctctgc ttcgtagagc atggtattaa tatgctgcgg ctgggtgaga
    14581 tcctgcatct ggctgccacg cggcacgaag cacactttct ggcattcaga cagtttttca
    14641 atcaccagcg atcccgcttc gccctgaatc tcgcttgcca ggacagaatc actgaccttt
    14701 gagtgctgca aggtgacgct gaaatcaccg taatccatca ccaccacgcc atgggcatct
    14761 acaccacttg ccagtaaact ggcggttgcc tgcacgcttt tcggctcacc aaataacgct
    14821 accgccgacg ccaggcagta aaagccgata tccataattg aaccgttaga gaatgccgga
    14881 ttaaaggtgt tgggattctc accatccagg taacgttgat accgcgagga atactggcaa
    14941 tagttgaaaa agactttccg cagtttgcct actttcggca gcgcctggcg cagcaaatga
    15001 aagttcggca ggctggctgt tttaaatgcc tcaaacagca ccacctgatt atcccgcgca
    15061 caggcaatgg cggcatccac ttccgccaga ttcgacgcca gcggtttctc gcaaatcaca
    15121 tgaattttgt gactaaggaa aagttgtgcc tgggaaaagt gcagggaatt cgggctggca
    15181 atatacaccg cgtcaatggc atcgctttcc gccatcgctt ccagcgaggt aaacagatgc
    15241 tcgacagaaa aatcattggc gaagtgctga gcctgctcaa ggctgcggga ataaacggcg
    15301 gttaacttgt atttaccgct ctcatgggcg gcctcgacga actggcgaga gatccagttc
    15361 gtaccaatca cagcgaaacg tatcataacg cgtggatact ccataaaggg attctgtcgt
    15421 caatgtagca cgcctttttc atcttcgacg tgcgcagata cgcattattc tttcgccgga
    15481 cagccatgct cactaaacgg cccaatcatg accgatggcc cgagatattt cggctggcga
    15541 tggaggattt tggcgtcaat caccgcgctg acacgagcga gtttctcccg cagttgagta
    15601 tataaaccat gcttcatatt caggtctggc gcgttaaacc cgatagcgtc gatgtcgtac
    15661 tgttttgcca gccagatggc gcgttggtta tgaaattctt gcgagataat ggtgatatgg
    15721 ttttccccaa acaccttttt ggcacgcact accgaatcca gcgttgagaa tccggcatag
    15781 tcgcagaaga tcactttcgc cggtacgcct ttagcgatca aagcctgctg catacctgat
    15841 gcttcatcgt aatttttacg tccgttatcg ccactgacta acagccattt cacttttccg
    15901 gcgtggtaca actctgctgc ggtatcaata cgtcgggtaa agtagcgatt acccggcctg
    15961 gcccccagca ataagcccac gttacgcgcc ggaacgacgt tgacgtcgct ccaggtcagc
    16021 tgtttgctgg cgttgaccat tacgcgatcg gcgataaaaa tggtcgctcc tgccacaagt
    16081 aacagtaagc aggcaatctt tagagtccgg cgagaaaaac aaatacggag aagaaggcgg
    16141 gcaaatgcgc gcagcatggt cgttatcctt aacgaaaggg gggaggtcga ttttaggcgc
    16201 taaagaaaga aaagataacg cctgacatca ttttcagatt ttgtcctgcc caagcgacaa
    16261 ctgggttagc cacagtcggg tgtcgaactc aagctggtgg tactggggtt ccatatgaca
    16321 gcacaactgg taaaacgctt tgttatgctc tttctccttc aggtgcgcca gctcgtgcac
    16381 cacgatcatg cgcaaaaacg gttccggcgc gttgcgaaac acggtagcga cgcggatctc
    16441 tgcttttgct ttcagcttgc cgccctgcac acgagaaaca gcggtatgta acccgagcgc
    16501 gttttttagc acatggatct tattgtcata catcacttta ttgatcggcg gggcattacg
    16561 cagaaactga tttttcagat cctgagtata ttgccagagg gctttatcgg tggcgtagtc
    16621 gtgtgttccc ggatagcgtt ttgccagcac atcgcccaga cgctgttcgt taatcagtgt
    16681 tcgcacctgg gaaagtagct gctcgggata accctgaaga taagtaagat tgctcataac
    16741 cgcccacgaa aaagagaaaa agggtatact cacgcaccct tttcaggggc aatgtcgaaa
    16801 ttttatcatt caggagggcc gatgagccac ttagacaacg gtttccgttc actgacacta
    16861 caacgttttc cggcgacgga tgacgttaac ccgctacagg cgtgggaagc ggcggatgaa
    16921 tatttgctgc aacagttgga cgacacagaa atccgcggcc cggtgttgat cctgaatgat
    16981 gcctttggtg ctttaagctg cgcgctggcg gaacataagc cgtacagcat tggcgactca
    17041 tacatcagtg aactggcgac gcgcgagaat ttacgcctca acgggattga tgagtcgagc
    17101 gtgaagtttc tcgacagcac cgccgactac ccgcaacagc cgggtgtggt gctgatcaaa
    17161 gtgccgaaaa cactggcatt gctggaacag caactgcgtg cgctgcgcaa agtggtcacg
    17221 tcggatacac gtattattgc cggtgccaag gcccgtgaca ttcacacttc cacgctggaa
    17281 ctgttcgaaa aagtgctcgg cccgaccacc accacgctgg catggaaaaa agcgcgcctg
    17341 attaattgca ctttcaatga accgccgctg gccgatgcgc cgcagaccgt tagctggaag
    17401 ctggaaggta ctgactggac tatccacaac catgcgaatg tcttctcccg caccgggctg
    17461 gatattggcg cgcgcttctt tatgcaacat ctgccagaga atctcgaagg tgagattgtc
    17521 gatctcggtt gcggtaatgg cgttattggt ctgacgctgc ttgataaaaa tccgcaggcg
    17581 aaagtggtgt ttgtcgatga atcaccgatg gcggttgctt ccagccgttt gaacgttgaa
    17641 accaacatgc cagaggcgtt ggatcgctgc gaatttatga tcaacaacgc gctctccggc
    17701 gtggagcctt tccgctttaa tgctgtgctc tgcaacccgc cgtttcatca gcaacatgcg
    17761 ctgaccgata acgtcgcctg ggagatgttc caccacgccc gccgctgcct gaaaatcaac
    17821 ggcgagctgt atatcgttgc caaccgtcac ctggattact tccataaact gaagaaaatt
    17881 ttcggcaact gcaccaccat cgccacgaat aataaatttg tggtgctgaa agcagtgaag
    17941 ctggggcgtc gtcggtaaga ttgacagcta cctgccagat aaggcgtaaa cgccttatct
    18001 ggcctacgtt cagcacgtta cccgtaggcc gaataatatg cgtcgcatcc ggcgcttcaa
    18061 ataacgattt aaatctccag cgcaagccgt gtcccctggg caattgcccg tcgtgcgtcc
    18121 agctccatag ctacatcgca gccgccaatt aaatgcacag ttttcccgct atcaatcagc
    18181 ggttgcgcca atgcgcggtt tggctcttgc cctgcgcaga tcaccacatt gtccactgct
    18241 aatacctgcg gttcgccgtt gatcaccaca tgcagcccgt catcgtcgat cttctgataa
    18301 cttacacttg ggatcatttt cacgccgcgc gagagcaggg tggtgcgatg gatccagccg
    18361 gtggttttgc ctaacccctg tcccggtttg ctggctttgc gctggagcat cacgatctgc
    18421 cgtgggctac gagggatctg cattccctgc gggcttaagc caccagcctg ttgtaggcta
    18481 ctgtcgatcc cccactcatt acaaaactca gcgatattct ggctggtgga ttcgcccggc
    18541 tgacttaaat acatcgccgt atcaaaacca atcccgccac aaccgatgat ggcaactttg
    18601 ttacccaccg gcgctttgtc gcgcagtaca tcgagataac tcaatacctt cggatgatcg
    18661 atcccgtcga tgggcggagt gcgcggcacg atcccactgg cgaggatcgt ttcatcgaac
    18721 gcctgtaact gatccgccgt cacggtgtga ttgagtttta gcgtcacgcc cgtcacttcg
    18781 atcatccggt ggtaatagcg cagcgtttcg taaaactcct ctttgccggg gatctgtttg
    18841 gcgatattaa actgcccgcc gatctcgctg tgggcatcaa acaatgttac ctgatgccca
    18901 cgcgccgccg cgttaatggc aaacgccagc ccagcaggtc ccgcaccgac caccgccaga
    18961 ttttttttct gcacggcggg aaggattagc attttggttt cgtggcaggc gcgaggattc
    19021 accaggcacg aggtgacctt gccaacgaag atctgatcga gacacgcctg gttgcagcca
    19081 atgcaggtgt tgatctcatc ggctcgccct gattgcgctt ttgacagcag ctccgcatcc
    19141 gcaagaaacg gtcgcgccat cgataccata tcggcatcgc cgcgcgagag aatatcgtcg
    19201 gcaacctgcg gatcgttaat ccggttggtg gttaccagcg gcagcgagac gtggcctttc
    19261 agtttgcgcg tgacccagct aaatgcgccg cgcggcacgg gcgtggcaat ggtcggaata
    19321 cgtgcttcat gccagccaat gccggtgttg ataatggtcg cgcccgccgc ttcaatggcc
    19381 tgcgccagct ctaccgtttc ggcaaaagtc ccgccgtctt cgaccaggtc gagcatcgac
    19441 agtcggtaga taataatgaa gtcgttgcca acgcgttcgc gtaccgcact caccacttca
    19501 acggcaaacc gcatccggtt gtggtaatcg ccgccccact ggtcactgcg ctgattggtg
    19561 cgcagcgtca gaaattcgtt gatcaaatac ccttcggaac ccatcacctc tacaccgtcg
    19621 tatcctgcct cccgtgccag ttgcgcgcag tgggcgaaat cgtcgatcag ttgcaggatc
    19681 tcttcatggg tgagttcgtg aggaacgaaa cggttgatgg gggcctgcaa tgcggaggga
    19741 gcgaccagat gcggttggta gctgtagcgc ccggtatgca aaatttgcag ggctattttg
    19801 ccgccttcct gatgtaccgc ttcggtaatg gtgcgatggt gtgggatctg gctggcgtcg
    19861 ttgagcattg cgccaccttc catgccaacg cctgttaaat ctggtgcaat gccgccgcta
    19921 acaatcagcg ccacgccgtg acgggcgcgt tcggcataaa acgcagccag ccgttcagcg
    19981 ccgtccgggt attcctccag cccggtgtgc attgaaccca tcaacacgcg gttttttaac
    20041 gtggtaaaac ctaaatccag cggggcgaac agcgacgggt agctcataaa attgtccagt
    20101 atgtaaaata attgttatgt ggtcggatga gttctaattt agccgtcgcc ggagaaaagg
    20161 gaaaagggga gtgcggggtt tgtgatggga ttcaaaagga atacgcccgg tggtaacgcc
    20221 ggatgcgacg cttgccgcgt cttatccggc ctgggggatt gcacatgtag ggcggataag
    20281 gcgttcacgc cgcatccgcc agtggccgcg gtgcagatgc cggatacgac gcttgtcgcg
    20341 tcttatccag cctacgggaa cgaacatgta gggcggataa ggcgttcacg ccgcatccgc
    20401 cggcgcggtg cagatgccgg atgcgacgct tggcgcgtct tatccggcct acgggaacga
    20461 acatgtaggg cggataaggc gttcacgccg catccgccag tggcgcggtg cagatgccgg
    20521 atgcgacgct tgccgggtct tatccggcct ggggaacata catgtagggc ggataaggcg
    20581 ttcacgccgc atccgccagc cgttgcaaat tattgcttac ggaaaaaatc gttatacaac
    20641 atatacaaat gcgcggcact ccaggagaaa tttggtgcgc cttgctgcgc gccagtcagc
    20701 gggttgtaat tctcctgaat tggtccatcg gcggttaacc ctttggcgtg ccggaagaac
    20761 gtatccgcca gcttcagggc atcatcgcga taaccgtaac gctccatccc tttcagacca
    20821 aaccagaact gatccaccca tacgcgcccg cgccagtaga tatccgcgcc aaaggccggg
    20881 ttggttaacg ctgccgttcc cagcgggaca aaggtgttga actctttagg atcgagcatc
    20941 accttcacca ccgcgtcggc gttggcctgc gttgccgcac cgttaaacag cggcgaccag
    21001 ccttccggcc ctttaccgcg ctcaacaatc ggtttgcccg cgcagccgtt cgccagcggt
    21061 ttatcttcaa tacgcacgtc atagaagtac tgcgtagccg ggtcgaacat acaggtgttg
    21121 atgtagtccg cgagctgctg tgccaactgg cgatagcgct tagcctcttc cggtttaccg
    21181 agaatcgtcg ccatctccgc cagataatgg ttatcgctgt acatatagct ggcctgatcc
    21241 accgactcct gcaatagcga gtaccccagc aacgttccgt cctgactgcg gttttcggcg
    21301 aatttcaccg tccagtcgct acgtttgccg ccgcttgcaa catatttatt cagctgttct
    21361 ttgtcgataa acccaaagac ggcggcatca tcacgccccg attcccacga cgcagcgacc
    21421 tgtgccggaa tttccagact gtcgtactgg cctttctcca ccacgcgggc gtagttgttc
    21481 agcccagact gcgtctcttc tttatcgcct tttttcaccg taaacagcat ctcgccgctc
    21541 tcagtgttgt gggctttgtc gcgggtcgcg ccatattccg gcacgccgtt gccgttgtga
    21601 tcgcggttac gtaaccacca gtcgtgatag gccaccagtt tcgggtacat ctctgccagc
    21661 caggttttat cctgggtgac gttatacact tccatcaccg accaggcggc aaggctgggt
    21721 ttggtattac gttcgttcca gttgccgcca tcgccaccac gctcggggct aaggttccac
    21781 gctatcaggt cggggacaaa gcccacatcc tgcggacgca cgctgtcgcc gggctggatc
    21841 tgccaggaga agaccgcgcg gatattctct ttggcgatgt ccggattgaa atgcgccatc
    21901 gcaaacgcct gcttccaggt atcccacggc caggtctgat tgccggagaa ccagcgcccg
    21961 gtcaccgacg gtgtaatggt gttaaatttc accgcaccgc ccggtgagcg ccagttaccg
    22021 ttgagcgttt cgatcgcttt caccgcgacg cgcgtctgtt ccggcgtcgc atccggattg
    22081 gttaaccctt tcttcaggta ttcttcccag cgttgctgcg aggcggtgag ataaaacgcc
    22141 ggacgcgcca gaatatcgcg gatctgcatt tgctctttgc taacttcctg atcggtcagc
    22201 aggtgggagt aggtggtgta gagtgtggtc gaaccgttga tatgcgcctt actggtaaag
    22261 cgattgccgt tgatttcagt ctgcaccggc agggatttat gcacctgata ttctgattca
    22321 ccggaggtca gcagatccca agtggcgcgc actctgccaa aggtgacttt caggccatca
    22381 cgggtggcgc tgattttgcg ctggtagtca gggtattcgc cagcaatggt tttatcggaa
    22441 agcggtttcc cttctttcgc ttccagtttt tccagcagtt cgccatccca caccagatcc
    22501 agcggtttat tgctgatgat tttagtttcc agtagtgacg tgcgcggcgt ggcgaagcgc
    22561 agagtcattt cgacctgcac atcttttgct gtcagttttt gcaccagcgc gccgggaata
    22621 ctgtatgcct ccagcgtgaa gttgactttc ttgccgtcct gccagacggt caggcggtcg
    22681 aaattgctgg ccataaagtt gatgtattct tccgtcagca gcgcaacgcc cggaaagccg
    22741 cccatggtgt tcgggccgtc tggcaacaga tgaccatgcc aggctccgag atcgaaaaac
    22801 ggattaaaac gctggtgatc gtcgtaatcg taatccttca tgtactgcgg cgcaccagta
    22861 cggttaatca cgtttttata attgtcggcg ttagcggcat gggcggaaaa acttatcagc
    22921 agagcgcagg ttactggcgt taaaatagtt ttgattttca tgaaattaca gtccttatta
    22981 ccagtagaaa tattgcatca ggaagatttg gtcgctggga tcgttagagg catttttcat
    23041 gtagaagcgg ctatcaaaag ccgttgcgaa acgtccgccg ccatattcgt accagatacc
    23101 aaactgcatg aaagaggtgg tttcatcgtc agcgtggtca gggcgatgtt tggcgtaggt
    23161 gtaagcggtg gagagataaa cgccctttaa cgcttcatac atggcgctca ccatcatccc
    23221 gctttcactt cccggtacgt ccagcccgtc ggcacgtccg gtatggtgcc aggcgcgagc
    23281 ggcaaagtta ggggtaagca ggcccgtgag atagagttgt cctgtgccgt cggtaatttc
    23341 cagaccgttc atccaggtga tgccttcggc aaggtcatac tggatgtaac cgttcatcat
    23401 tgccggatag gtgtatttgt cgtcgtagcg atcgtacttg ccaaaatgta gccatgcttt
    23461 gctttcgtca tgatgtccgg caggtgtggc ggtgacgcta taacgaaagt tgccgcttaa
    23521 gttctgcacc ttcagcgcgg tcataatgtc gcgggtattg ggaatgacgt agcccaaatc
    23581 gggtgtgaag tcgccccacc attgcaggtc atccagcgag gaatcctgac gcagactgag
    23641 catgatttcg gtgccatcat tggtacgata accgccatag aaacggttaa tcccaccttc
    23701 gaaaccaccc cagctatggt caggcaccca ggcattgccg tcgtggtcgg cctgcacggt
    23761 ccagccttcg ccgtaaatca ggccgaacca ggagccgtac ttcatctcca gtccaccttc
    23821 aatataggtg ccgtcgctgt atttatgttt attctcaccc tctaaatcca tatatccacc
    23881 aacccctaat tcgccgtaaa aacgaaatga aggagaagat gtcgaggtaa ttgcggatgt
    23941 ttttgtgtcg tcatgttctg ccggacgtgc ttcttctcct gccgcagaaa aggcgtagca
    24001 aaacggcaga gcaagtaatg ctctgcatgg tgcagtgatt aatttcattg tgttaatcca
    24061 tgagctaaag aaaaagtaca gccttttatc gttgtaatta taaaaatata aaggcagaaa
    24121 ctattttccc tctcctcatc caggaagagg gaaaatgcct gctcattttt tattattctg
    24181 cttaatcaac attgctggct ggcgtcgctt caatatggcg ctcttcagcc gttaatccct
    24241 ttatatattt actgtatttc caccacgcaa agccaaggaa gataacaata ccgccgacgt
    24301 tataaaaaat gatggtcaga atattcgcgc cagtcgggaa tgtcgaagcg acaaacccta
    24361 cggcaaatat cgcaatcagc attgaaacaa cgataatacc ggtgcggcgg gagcccatac
    24421 ggaaatcgcg tggcaggtga tctaatttgg cgcgtaaatt caggtaagcc agcatgatga
    24481 ataacggcgg aagcatggac gctgcggcgg tcatattaat aatagtattc atcagatcct
    24541 gcacggtatt ggaaccgagc atcgggataa tcatcagcgg gatgacgatc aggaactgga
    24601 tccacgctgc gcgcgccgga acgccgtttt cgttaagttc gacggttttc ttaccaaaga
    24661 tgccttccgg gatttcggag aagaaaattt tcaccggcgt tgcggtccac atcagcaggg
    24721 aaccgaacat tgcggtaaag gacaccagac cgacaaagcg attcatcaac gcttccggca
    24781 gaccaaaata cgccgccatg ccgtggaata cctgcaccga tccgccggta aatttcaact
    24841 ctttgctgct gacgaagacg ttaatcagca ccgaggagac ggaatacagt acgccgataa
    24901 aaatcccggc gaggatgatc actttaacga acgatttcga accgcctttg acgtcgttaa
    24961 cgtacaccgc gacggactcc gcgccacctg cggccataaa gatccaggtg gtaacgccga
    25021 ggaacgccca gttgaagttc gggatcatcg cgtcaacggt gatcggatcg gcaggctgta
    25081 cgccgccaac cagtgccgta cctgccagta aaatgtagga gagcgttaac agcagcatca
    25141 gcgttgaagt gacggaggta attggcccca acattttcgc cccgttggtg gaaacccagg
    25201 tggagaaggc gaacagcacc atactgataa tggtggtggc aaccggcgtc ataatatatt
    25261 cgtatccgag gaaggcatac gaagcataag caataacgcg cggcaacagc gaggtgaaaa
    25321 agaacaggtt tacgaaccag taggtatagg cagtaataaa tgcccaacgt ccgcccagcg
    25381 aactttttac ccacgcgtag acaccggctt ctgagttttt atttaacgaa acaaattctg
    25441 cgatgatcag acagaaggga ataaaataaa aaatcgtcgc gaggaaaaac atcggtgccg
    25501 aggccagtcc aagctcaata ttattattga taacgttgtt aaagctgtaa acggcggcaa
    25561 aagtcagcga gagcaagccg aatttgccga ttgtattacg tttggtatca gacataacac
    25621 atctccgaaa atgagtaggg tagcggtggc ggcaaactcc ttttgccgcc gtagttgtta
    25681 tttgttatga aaataaccat cttcgatggt gactttgaga accacttttt tgaccgcgtt
    25741 attgcagata aaccgatatg cttcatggat atcgcaaata acgatttgcc cttcgtggac
    25801 ctcaacggtt tctccgcagc cttttaaata ttcacagtca gtttcatcac gataatattc
    25861 cacgacctgt aatgtctctt tcggcgcata ttcaattttt tgctgccctt gcaggtagta
    25921 atgcacttca aaatagcgtc gatgcccggt aaatagcgca tcggtcgcgg agtctgtctc
    25981 cacgcggtaa gtcaatgagt caccgatgga gtgggcgacg ccaggctgaa tgttgtcgat
    26041 attttcaatc gcttcaacgc agcgttgcca cttcttacca gaggcgtaaa tctggcggaa
    26101 ctgttctaag ttatcgatga tcctcattgc ttattctcgc tgtgcaaatt cgtggaaaag
    26161 aaccctgcgc cgaactcata cgacgccagg ctttgcgcgg tagcttctcc gccagaaacc
    26221 ggcagcaacg taaagccgta gctgaagtca cggaaccaga cgcgccagga gtccagcacc
    26281 tcgctgcccc aggagttgga gccgaggcca agcagctggt ggtcgagatt cagggtgatg
    26341 tcatcactgc gctgcagctc gttacagtgc tgggcagcgt ggatgttttc ctgggtatag
    26401 tgccaggcgc tgaagttaat tgggcgctgc ggaaccacca gcagaccgtt gccgtggcgg
    26461 ttagtcagtg ccgtccagcg gacatgctga aggttgccgt tgttctgcgg gaagggatag
    26521 ttctcgaaca tggcatcgac ggtgctgcgc cagatatcga tgatgttagc ctgctggctg
    26581 tcggcgtagt tttcgcccgg tccacgaccg taatacgcca cctgatcgta ttcgccgtta
    26641 atccccatgg tgaaaccgat gcacggaatg atgtgcggat agtcgccgta acgctcgccg
    26701 gaaagcgcca cgttaacctg gccatcggca gcgatgcgcc agatgtaggt gcagcgcatc
    26761 ccgaagtcaa acaccggcgg tgctataacc gtgcggctga tgatcaacac ttcaccatcg
    26821 ctctgttcta cggcaaagtc gcgcagatgt tcctgcatga tctgcaaatg attcggctgc
    26881 cacaaccctt cgtactcctg cttgtggttg tcgatcatcg gcttgaagaa gttgatcttt
    26941 ggctcgcggg tcagcagcga ctcgccattt acctgccagg atgtcggttt gccactcatt
    27001 tttgagaagg tgatcgcgaa gttgtagccg cgaacggtgc agctcaaacg atcgtcttcc
    27061 agcgtcagcg gacgcgcatt atttggtgcg aaaggcactg gctgcgcggt gttttccttc
    27121 agcgggaact gataagtggc gatagaatgc ccggcttcgc tgtagcgggt gcgggaatct
    27181 ttggtcacca taatgttgag gaacgcttcg cgggcgtcca gctgcggcag cgtgatctgc
    27241 aagggggctt cgctgttcgg cgcaacgtcg cgcagtttaa tctgctgcgt cgcgagcgtt
    27301 tcaccttcgg cgcgcacctc tgcgtgcagg gtgtagtcat caagcgtggt aaaccacagt
    27361 ttattttcga ctttcagctc gccgcgagtc agatccagcg cgtggatttt taccggcgcg
    27421 ataacctgtt tgtactcttt caggcccggt cccggcgtct gatcggaata gatcaaacca
    27481 tcaagacaga agttatagtt gttgggatag tcgccgtagt cgccgccgaa tttataccag
    27541 acattgccgt tgtcatcctg cgcctggatc ccgtggtcgc accactccca aacataatgt
    27601 ccctgaatac aatcgtgctt atagaagacg ttctggtact ccgtcagccc gcccggtccg
    27661 ttccccatcg catgagcata ttcacagatg atgcgcggct tcggatgcgg gtattcacca
    27721 aactcattca tcagcggcac gcgggtgtac atggtggaaa taatatcgac cacttcagca
    27781 tcgcgatctt cttcgtaatg caccagtcgc gtgtcatcca gcgccttcgc tgcgtggtac
    27841 atcgcgcgga tgttacagcc atagccggat tcattgccca gcgaccagat gatgatcgat
    27901 ggatggtttt tctgcgcgtg aatatggcga acaatgcgct cgacgtagac tttttcccac
    27961 tgcggatcgt cggtaatacg gctgatatcg ccgacattag caaagccgtg cgattcgacg
    28021 tcggtttccg ccatcacaaa caagccgtag atatcacaca gttcgtaaaa acgcggatcg
    28081 ttcgggtagt gagcggtacg cacggagttg atgttgtgtt gcttcatcaa ctggagatct
    28141 ttctcgacgc gatccattcc aacggcgcgg cctttgcgat gatcgttgtc gtgacggttg
    28201 acgccgtgca gcatcacata acggttattg atccagaaca gaccgtcgcg cactttgata
    28261 tcacggaagc caacgcgttg tggcaccact tccagaacgt tgccgtcggc gtctttcagc
    28321 gtcatgacca gatggtaaag ataaggggat tctgctgacc attgctgggg ctgttcgaca
    28381 gtaaaagcaa agctggcgct ggtcagtttt tcaattgcca aatgatcaat ggtgctgctg
    28441 tgcaccacgc gttcgccatc aaacagggta tattccagcg tcgtgacgac aggggaggct
    28501 gcgagatttt ccagcaccac ttcgcaggaa agcgtggcat cgcaataggc ttcgtcaaag
    28561 tcggtacgca cggtgaaatc gttaatatgc gttagttgtt ttccgaccag ataaacatcg
    28621 cggaagatcc ccgctgacca ccacatatcc tggtcttcca cgtaggtaga gtccgcccac
    28681 tgcatcacac gcacacacaa caggttgtcg ccggttttaa ccatcgcgct gatgtcaaac
    28741 tctgcggtca ggcgactgcc cttgctgaaa cccacatact gaccgttaac atagacttca
    28801 aaataggttt cgacgccgtc aaatttaatc agcgtctgtt taccctgcca gccgtcgctg
    28861 agggtgaaaa tacgttgata ggcaccggtt gggttatcgc tggggacaaa cggcacatcg
    28921 atggggaacg gaaaaccttc gtcggtatat tgcagtttgc cgtgaccttc catttgccac
    28981 atggcgggga cggtaatatg cccccagtca gccattaact cagaggtgaa ggcttctggt
    29041 acttgcagcg gatggtcaaa aaagtggaaa ttccactgac cgcttaaggg cagaaacagg
    29101 ctgctggttt cgcgggcaaa ggtacgcgct tgcgcaacag aatcatatga aaaaaagtac
    29161 gcacgcggcg caagtcggtt ttcgtgggtg agctgaatgt tttcccagcg attcataggg
    29221 cctcccggta gagagaaatc ggaaccattc atggtgatgg tagtttgtgt aattgctggg
    29281 gagtttagta aaatttttac taaaaagtag caggtaaatg catttacttt aactggatca
    29341 cgaaaataaa cggcagcact gtccggcaaa aacgactgcc ggactgtgtg taaggggatt
    29401 taacgggtcg tgccgcgcag ttttaatttg ctgggaacga agactaacag cggcagcgcg
    29461 cgaccatcgc gggctttttc atacaccagg ttaacgccct gacttcccat catttcggaa
    29521 tggatgcgca cggtggagag cggcggaaag gtaaatcgcg cggtggggat atcattaacg
    29581 ctgataagcg aaatatcctg tgggatgttc agaccacgtt catgaatcgc ccgcagtacg
    29641 ccgatagcaa tggaatcgga agcaacaaac agtgccttcg gatagtcttc ccgcgccagc
    29701 atctgttttg ccagttcata acccgacgaa ctggaaaaac cgccgcgcca gatatcctct
    29761 tcgcgtacta cttgtttcag acggccatat tccgcaaagg cgacctcacg aatatccgcc
    29821 ttgccaggct catcttcacc gccaataaaa ccaatacgat taacgccctg gttgatatag
    29881 aagtcgatga tttctttact gatgcgtgcc agatcgatat ccaccgcatc gtaaccgctg
    29941 ccgggttcgt gaaagtcgat aaaacagata ttgtcggtca acgcacaggc ggcggcgcgc
    30001 agggcgggcg tgggtttgcc gacaattaaa ataccggtga cgtttttaat gtctggtaat
    30061 ccgttgtgtt cataacagtt ggtcagttcg atcgccagct tttcgcactg agtttcaatg
    30121 ccgtggcgga tcgccagata gtaaggatcg ttgatctcca gctcctgctg gtagctgtag
    30181 atagccagaa tatggtgttg gttgactgca cctgtctgga gtttacgggc gctactggtc
    30241 ttgtactcca gcttttcggc gatctcgaga atgcgatgtt tcgtctcttc tttcacattc
    30301 aatgtcggat cgtcatttaa gaccctggat actgtcgcca gggatacgcc agcttcgatt
    30361 gcgatgtctt ttagtgttgc catgtttacc ttttttactt tccctgatgt gcgcttccat
    30421 tgtagaggaa gtgcttatcg ggtcacctat tttcagtaaa taaagcgcgt ttctttgacg
    30481 ctgtaacggt aacacagcgg ctcacgaatg tgatcacgcc tgcattctcc atttttagcc
    30541 tacaggattg agaaaaactt ttgctatagt ttagtaaaat tttactcgaa gggaggaagc
    30601 aatggaaacc gtggcttacg ctgattttgc acgtctggaa atgcgcgtcg gaaagattgt
    30661 ggaagtgaaa cgccatgaaa acgccgacaa gctgtacatc gtacaggttg atgtggggga
    30721 aaaaacactg caaaccgtga ccagcctggt gccgtactac agcgaagaag agttgatggg
    30781 aaaaacggtg gtggtcttat gcaatctgca aaaagcgaaa atgcgtggtg aaacgtcgga
    30841 atgcatgctg ttgtgtgcgg aaaccgatga cggcagtgaa agcgtgctgt taacgccgga
    30901 gcggatgatg cctgcgggcg tacgcgtcgt ataaaaagat cggatggcga cgtcgtatcg
    30961 ccatccgatt tgatattacg cttcttcgac acttactcgc atggccgcca gcgccttacg
    31021 cgccgctttg atcaccagtt cacactgttc aatggtcagt gtcagtggcg gttcaatgcg
    31081 gatcgttttg gcgttattga gcgttccggc caccagtacg cgctggcgga acatctcgct
    31141 ggcaaagtta tagccgattt cgttatcaac aaactcaatc gccattaaca tccctttacc
    31201 acgcgcttcc tgtaccagat cgggatattc ccgcgccagt tgacggaaac cgtccagcaa
    31261 catatcgcct ttttgttcag cctgagccgg taagttctgc tccagcaaca cattgatggt
    31321 cgccagcgcc gccgcacagg ccagcgggtt gccgccaaag gtggtggtat gcaggaacgg
    31381 gttgtcgaac agaactgaaa acacctcttc agtggcgatg gttgcgccaa tcggcatcac
    31441 gccgccgccg agggcttttg ccagacacag aatgtccggc tgaacgttct catgttcgca
    31501 ggcaaacatc ttgcccgtgc gccccatgcc cgtttgtact tcatcgagga tcatcaatgc
    31561 gccgaactca tcgcacagct tacgcacggc ggtgagatag cccggcggcg gcagaattac
    31621 gccaccttcg ccctgaatcg gttcgaggat caccgcagcc acatcatcac cggttttttt
    31681 gcactcatta agagccgtgc gcatggcttc gatattgcca aacggcacat gacggaaccc
    31741 tggcagcagt ggcataaacg gtttgcggaa ggtcgatttc gccgtggccg acagcgcgcc
    31801 aagtgattta ccgtggaacg cgccgctggt ggcaataaaa gtaaatttgc cacgcggcga
    31861 ctgataagcc ttcgccagct tcagcgccgc ttcaacggac tcggtgccgc tattacagaa
    31921 gaagctgtat ttcagtttac cgggcgttag cgcagcaagg gttttcgcca gcatcgcccg
    31981 taacggatcg agcagctcct ggctgtgcag tggttgtttc gcaagttgat tctgtacggc
    32041 ggaaaccaca actggattac ggtgccccac gttgaaaatt ccaaaacctc ccaggcagtc
    32101 gataaactcc tgtccctggg tgtcgacaag cgtatttaaa cctcccgctt gccactctac
    32161 ggctccgtaa tccccgccgg cggtaacaga tttgcgatac tctaaaaacc ccggattgac
    32221 atgctctttg aagtattcaa tcacctctcg gttaagtgct ttcatctcct catgatccag
    32281 cgttcgcttt tcaatgagat tcagggcgtg ggcgctgcac gctaaagccg atgcgctcga
    32341 aggtaacctg ttcaaaatat gctccggagg ctcgcgtatc acatgatacc gatttaagta
    32401 ttgcagggat tgcgccactc cggctgcgat cgcgaaaatc gggataaaca caaatgtggt
    32461 tatcatttgc gcaatattta atgctatttg ttaacatacg ttgttatttt gcacggcatt
    32521 tgtcgggtat ggatttgata attgctgcgc aaatgcacgt tttcagggca ataattgcct
    32581 gatttttggg caagataatt aacgttttcg tatagtaaca gagtgaaagt attttaaatc
    32641 aacgaattag atttgtgctt taatcgcaaa ttgcgatcta aatcaaatta atcggttaaa
    32701 gataaccgca gcggggccga cataaactct gacaagaagt taacaaccat ataacctgca
    32761 caggacgcga acatgtcttc tcatccgtat gtcacccagc aaaatacccc gctggcggac
    32821 gataccactc tgatgtccac taccgatctg caaagctata tcactcatgc taatgacact
    32881 tttgtgcagg tgagcggcta taccttgcaa gagttacagg ggcagccgca caacatggtg
    32941 cgtcacccgg atatgccaaa agcggcgttt gcggatatgt ggttcaccct gaaaaaaggg
    33001 gagccctgga gcggcatcgt gaaaaatcgc cgcaaaaatg gtgaccatta ttgggtgcgg
    33061 gccaatgcgg taccgatggt gcgcgaggga aaaatcagtg gttatatgtc gattcgtacc
    33121 cgggcgacgg atgaagagat cgcggcggtg gagccgctgt acaaagcgct gaacgccgga
    33181 cgtaccggta agcgtattca taaaggcctg gtggtgcgta aaggctggct gggtaaactt
    33241 ccttcattac cgcttcgctg gcgggcgcgt ggagtgatga ccctgatgtt tatcttgctg
    33301 gcggccatgc tttggtttgt tgctgccccg gtggtgacgt atatcctctg tgcgttagtg
    33361 gtattgttgg caagcgcctg ttttgaatgg cagattgtcc gcccgataga aaatgttgcc
    33421 cgtcaggcac tgaaagtggc gaccggggaa cgtaatagtg ttgagcatct gaatcgcagc
    33481 gatgagctgg ggctgacatt acgtgcggta gggcaacttg gcctgatgtg ccgttggcta
    33541 attaacgatg tctcaagcca ggtgtccagt gtcagaaacg gcagtgagac gctggcgaaa
    33601 ggcaccgatg aactgaacga acatacccag cagacagttg ataacgttca gcaaacggtg
    33661 gcgaccatga accaaatggc ggcgtcggtg aaacagaact ctgccacagc gtcggctgcc
    33721 gataaactgt caatcaccgc cagtaatgcg gcagtgcagg gcggggaggc gatgaccacg
    33781 gtgatcaaga caatggacga tatcgccgac agtacccagc gcattggcac cattacttcg
    33841 ctgattaacg atattgcgtt tcagaccaat attctggccc tgaatgcggc ggtggaagcg
    33901 gcgcgtgccg gcgaacaggg caaaggtttt gcagtggtgg cgggggaagt gcgtcattta
    33961 gccagccgca gcgccaatgc tgccaacgat attcgcaagc tgattgatgc cagtgctgat
    34021 aaggtgcaat ccggttcgca gcaggtacac gccgccggac ggacgatgga agatattgtg
    34081 gcacaggtga aaaacgtcac ccaattgatc gcccagatta gccattcaac gctggaacag
    34141 gccgatgggc tttccagcct gacccgtgca gtggatgagc ttaacctgat cacccagaaa
    34201 aatgccgagc tggtggaaga gagtgcgcag gtgtcggcga tggtgaaaca ccgcgccagc
    34261 cgactggaag acgcggtgac ggtgctccat taatcggtat gccggatccg gcggttggag
    34321 cacaatgcct gatgcgatgc tggcgcatct tatcaggcct acggggtatg tagcgggtgt
    34381 aggccggata aggcgcgtca agcgtcgcat ccggcaattg caccgcgcca ctggcggatg
    34441 cggcgattaa tccagttgcg taatatcaaa agcggcgcgg tcgatcaccg caatgatctt
    34501 ttttatttgt gcatcagtga tatcgctctg gttgacgcgt aaatccagca ccgctttaaa
    34561 attatccagc gcccgcttca tttgcgggtt ctggcgcagc gcagcgccaa cgcaacgcgc
    34621 tttgatgcgt tcttcaatca tctccacctg ttcgcggttt tcttccagcc actgcgcgcc
    34681 ttgttcggtc agtgcaatct gcttcttacc accttcctct tcgcggatgg taatcagcga
    34741 ctgctcctgc agaaaatcca gcgttgggta gatgacgccc gggcttgggg tgtaattccc
    34801 ctgggttaga ttctcaatcg ctttaatcaa ttcgtaaccg tggctgtcat cgcgcgagag
    34861 aatatccaga atcaccagac gtaattcacc gtgaccaaag aaacgttgcc gacgaccgcc
    34921 gccgcgaccg tggcgaccac cgcagcattg accgtgttca tgctggtgac cgtgtccgca
    34981 gtgctcgtgt tctgacttct cacctttgca gcagccctca tggcgtggct ggccttcatg
    35041 tttacaacac ccttcgtgat gatggctcat atcagcctcc ttttgcttaa ttagatatat
    35101 ctaatttata tctgatatga gcgctcttgc aagaataaaa atgattttta aatgtggact
    35161 catc
//
LOCUS       DAPGOF010000029        34974 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.029, whole genome shotgun sequence.
ACCESSION   DAPGOF010000029 DAPGOF010000000
VERSION     DAPGOF010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34974
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.029"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            109..705
                     /locus_tag="RHV13_001252"
     CDS             109..705
                     /locus_tag="RHV13_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV1240806.1"
                     /translation="MKKALLAAALVMASGSALAVDGGHIDFNGMVQSGTCKVGVVDTG
                     MHSVTTDGVVTLDTANVTDTFTEVSATAVGLLPKEFMISVECDPGAPKNAELTMGSAS
                     YANTSGTLNNNMNITVNGIAPAQNVNIAVHNMKNKAGAAEIKQVHMNNSSEVQELTLD
                     AEGKGQYVFNASYVKAPNSPAVTAGHVTTNALYTIAYK"
     gene            792..1517
                     /locus_tag="RHV13_001253"
     CDS             792..1517
                     /locus_tag="RHV13_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HDV1240807.1"
                     /translation="MFPLVKKTVSALFVSTLLASAPAFADIIISGTRIIYNADKKDVN
                     VRLENKGNRPLLIQNWLDTGDDNADPSQIKVPFTSTPPVSRVEPKRGQTVKVMYTGAT
                     ALPKDRESVYWFNVLEVPPKPKDAESDKNLLQLAFRTRIKLFYRPSGLQGEPAEAPAK
                     ITWKLNNAQLQANNPTPYYVSFNEVKLESGGKTYNVNSSMVAPFSQASFRVTGLPGSV
                     SSGKVIFKAINDFGGNIDGSATF"
     gene            1559..4159
                     /locus_tag="RHV13_001254"
     CDS             1559..4159
                     /locus_tag="RHV13_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HDV1240808.1"
                     /translation="MYQFTHQISRIPKKTLLAACCALFYSSNGAAADTVEYDSSFLMG
                     TGASTIDVKRYAQGNPTPPGLYNVRVFVNGQATSSLEIPFVDIGEASAAACITHKNLA
                     QLHIKQPEQPVTLLAREGEEEDCLDLAKSYEKADVRFDGSDQFLDLTIPQAYVLKNYG
                     GYVDPSLWESGINAATLAYTLNAYHTSSNNDNSDSAYGAFNSGINLGAWHFRARGNYN
                     WTTDNGSDFDFQDRYLQRDIPAIRSQIIMGDAYTTGETFDSVNVRGVRLYSDSRMLPS
                     ALASYAPTIRGVANSNAKVTVTQSGYKIYETTVPPGEFVIDDISPSGFGSELVVTIEE
                     ADGSKRTFTQPFSSVVQMQRPGVGRWDFSAGKVIDDSLRSEPNMGQASYYYGLNNLFT
                     GYTGIQFTDNNYLAGLLGVGINTSIGAFALDVTHSRAEIPDDKTYQGQSYRVTWNKLF
                     QDTGTSFNLAAYRYSTQDYLGLHDALVLIDDAKHLSADEDKNTMQTYSRMKNQFTVSI
                     NQPLKTTYEDYGSLFISGSWTDYWAANNSRTEYNVGYSKSVSWGSFSVNLQRSWNEDG
                     EKDDAMYVSVSVPIENILGGKRKSSGFRNLNTQLNTDFDGSHQLNVNSSGNTENNLVN
                     YSVNAGYSLDKNSGDLASVGGYLNYESGLGGISASASATSDNSQQYSISTDGGFVLHS
                     GGLTFTNNSFSSNDTLVLINAPGAKGARINNSNNEIDRWGYAVTSSVSPYRENRVGLN
                     IETLENDVELKSTSATTVPRSGSVVLTRFETDEGRSAVLNITAANGKSIPFAAEVYQD
                     DVMIGSMGQGGQAFVRGINDSGELIVRWYENNQTIDCKLHYQFPAQPQTLGSTNTLLL
                     NNLTCQVANH"
     gene            4173..4721
                     /locus_tag="RHV13_001255"
     CDS             4173..4721
                     /locus_tag="RHV13_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV1240809.1"
                     /translation="MKRILLTSALIGLGLPAVGSATDLNVDFTATVLATTCTITIVQD
                     GGPVVTGSNGEYSLTIPDVGLDKVITGDVAAQANFKLLASDCSNGYSKIFTTLTGASV
                     SGKLIVNEASSGATGVGMGIKRRDTADSTFFTPNNTDKFEWTTDEKANGVPLTVALRE
                     TTAGAGRTGAFQAKATFNFTYE"
     gene            4736..5347
                     /locus_tag="RHV13_001256"
     CDS             4736..5347
                     /locus_tag="RHV13_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV1240810.1"
                     /translation="MKLKVIATLIATVAVGASVSNNLAYAGTTSASLTVNSNLTMGTC
                     SAKILDTSDTVTDTVAFNNVYISELAAKSKVQPFKIRFSDCAGLPGKSAQLVLAPKGI
                     GCAGGMSNTAGFANKLNSTTEGGSSGAAVEVWTTTTPGGSGSKQFSCIQRNVVNVDLS
                     NASTTQPYDFDLSARMILAEGRTVNDALPGSFLSPTTFTITYQ"
     gene            5368..5958
                     /locus_tag="RHV13_001257"
     CDS             5368..5958
                     /locus_tag="RHV13_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV1240811.1"
                     /translation="MLCRHHKFVRFLGLVTALVTPFAYAGQDVNLTAQIVASTCQVEV
                     SNNGVVDLGTVTLDYFADNVTPTMDYAGGKTFNVNVVSCDNIQTTQSQMKLDFQPQAG
                     SLAQVNNQIFSNEYEQQATGAKNVGIVIFSAQPNQQTFNVRGTDGSSTAIYSVAPGNA
                     APSTWTFYSRMQRVNNALPPESGMVRSQVIVNVSYE"
     gene            5970..7082
                     /locus_tag="RHV13_001258"
     CDS             5970..7082
                     /locus_tag="RHV13_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV1240812.1"
                     /translation="MQRKGNKLLIQLCSAILLFFTTSWYALATECYIESNAAGAYDIV
                     INPTPFSIQSAMVTVPEEISFATWDVHIDLRGDKIGCKSLGSNTSAVHFLNTADASNI
                     STYTDSHGNGLLKTTVTGIVYSVELQCRNCGAAEDLSLMLPAQSGGDNHTSNSDTKWA
                     YEYSDDSWYLRFRFFWTPEYKPQNGVSEGFAIPGKIASWYIGNENQPWINFFVDTSSL
                     HFYVEEPTCQTVALAQDQGNVSGNQVTLGNNYVSEVKNGLTREIPFAIRAEYCYASKI
                     TVKLKAANKPSDATLVGKTTGSASGVAVKVHSTYEDSKVLLKADGSNTVDYNFAIWSN
                     NLLNLPFTAQLVPDGSGNAVGVGTFSGNATFSFTYE"
     gene            7217..8011
                     /gene="panB"
                     /locus_tag="RHV13_001259"
     CDS             7217..8011
                     /gene="panB"
                     /locus_tag="RHV13_001259"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HDV1240813.1"
                     /translation="MKPTTIASLQKYKQEKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITESLVIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            8023..8874
                     /gene="panC"
                     /locus_tag="RHV13_001260"
     CDS             8023..8874
                     /gene="panC"
                     /locus_tag="RHV13_001260"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HDV1240814.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRNVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(8970..9179)
                     /locus_tag="RHV13_001261"
     CDS             complement(8970..9179)
                     /locus_tag="RHV13_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000404125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1240815.1"
                     /translation="MEIKTRRETRQTLAQWFEEKGFQKGYQEELQKVRQEFAQRFLSK
                     GMSREDVAEVTTLPLTEIDKLINSN"
     gene            complement(9248..10120)
                     /gene="rpnC"
                     /locus_tag="RHV13_001262"
     CDS             complement(9248..10120)
                     /gene="rpnC"
                     /locus_tag="RHV13_001262"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HDV1240816.1"
                     /translation="MDAPNTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEERGRQEERQEFVLRLLNKGMSREDVAE
                     MANLPLAEIDKLIN"
     gene            10394..10774
                     /gene="panD"
                     /locus_tag="RHV13_001263"
     CDS             10394..10774
                     /gene="panD"
                     /locus_tag="RHV13_001263"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HDV1240817.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(10778..12007)
                     /locus_tag="RHV13_001264"
     CDS             complement(10778..12007)
                     /locus_tag="RHV13_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDV1240818.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQVRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMVQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(12071..12511)
                     /locus_tag="RHV13_001265"
     CDS             complement(12071..12511)
                     /locus_tag="RHV13_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDV1240819.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(12616..13386)
                     /locus_tag="RHV13_001266"
     CDS             complement(12616..13386)
                     /locus_tag="RHV13_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV1240820.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(13383..14309)
                     /locus_tag="RHV13_001267"
     CDS             complement(13383..14309)
                     /locus_tag="RHV13_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV1240821.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            14418..15080
                     /gene="can"
                     /locus_tag="RHV13_001268"
     CDS             14418..15080
                     /gene="can"
                     /locus_tag="RHV13_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HDV1240822.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(15121..15657)
                     /gene="hpt"
                     /locus_tag="RHV13_001269"
     CDS             complement(15121..15657)
                     /gene="hpt"
                     /locus_tag="RHV13_001269"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDV1240823.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            15863..18253
                     /gene="gcd"
                     /locus_tag="RHV13_001270"
     CDS             15863..18253
                     /gene="gcd"
                     /locus_tag="RHV13_001270"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HDV1240824.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPANGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLNQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(18455..20005)
                     /gene="cueO"
                     /locus_tag="RHV13_001271"
     CDS             complement(18455..20005)
                     /gene="cueO"
                     /locus_tag="RHV13_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HDV1240825.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            20171..20518
                     /locus_tag="RHV13_001272"
     CDS             20171..20518
                     /locus_tag="RHV13_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HDV1240826.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKSLGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            20624..21490
                     /gene="speE"
                     /locus_tag="RHV13_001273"
     CDS             20624..21490
                     /gene="speE"
                     /locus_tag="RHV13_001273"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HDV1240827.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            21506..22300
                     /gene="speD"
                     /locus_tag="RHV13_001274"
     CDS             21506..22300
                     /gene="speD"
                     /locus_tag="RHV13_001274"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HDV1240828.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(22338..22700)
                     /locus_tag="RHV13_001275"
     CDS             complement(22338..22700)
                     /locus_tag="RHV13_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HDV1240829.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(22876..25473)
                     /gene="acnB"
                     /locus_tag="RHV13_001276"
     CDS             complement(22876..25473)
                     /gene="acnB"
                     /locus_tag="RHV13_001276"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HDV1240830.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETNELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            25828..27585
                     /locus_tag="RHV13_001277"
     CDS             25828..27585
                     /locus_tag="RHV13_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HDV1240831.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVEPQSTQ
                     LAVSDAAAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSGQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVTQTVTAPVIPSN
                     TVSTASPVITEPATTVISIEPANPEVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGIATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDSNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAENYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQNQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRIQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(27656..29080)
                     /gene="lpdA"
                     /locus_tag="RHV13_001278"
     CDS             complement(27656..29080)
                     /gene="lpdA"
                     /locus_tag="RHV13_001278"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDV1240832.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(29288..31180)
                     /gene="aceF"
                     /locus_tag="RHV13_001279"
     CDS             complement(29288..31180)
                     /gene="aceF"
                     /locus_tag="RHV13_001279"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HDV1240833.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(31195..33858)
                     /gene="aceE"
                     /locus_tag="RHV13_001280"
     CDS             complement(31195..33858)
                     /gene="aceE"
                     /locus_tag="RHV13_001280"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HDV1240834.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(34019..34783)
                     /gene="pdhR"
                     /locus_tag="RHV13_001281"
     CDS             complement(34019..34783)
                     /gene="pdhR"
                     /locus_tag="RHV13_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HDV1240835.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT         8610 a         9247 c         8534 g         8583 t
ORIGIN      
        1 aatatattca cacaagtgat acctctcccc ctattacatc cttccccgac tttaagtaat
       61 atccgctcgc aatattaatt tcgtataatt tttgtaagga tgcatgtaat gaaaaaagca
      121 cttctcgcag ccgctttagt aatggcttct ggttccgccc tggctgtaga tggtggtcat
      181 atcgacttta acggtatggt acagtccggt acctgtaaag tgggtgtggt agataccggt
      241 atgcacagcg ttaccactga tggcgtggtt accctggata ctgcgaatgt tactgatact
      301 tttactgaag taagtgcaac tgctgttggt ttactgccga aagagttcat gatttctgtt
      361 gagtgtgatc caggtgctcc gaagaatgct gagttgacta tgggttctgc aagctacgcg
      421 aacaccagcg gtaccctgaa taacaatatg aacatcactg ttaacggtat tgcaccggct
      481 cagaacgtga acattgcagt tcataacatg aaaaacaaag ctggcgctgc tgaaattaag
      541 caggtccata tgaacaactc ttctgaagtt caggaactga cattagacgc agaaggtaaa
      601 ggtcagtacg tgtttaacgc atcttacgtt aaagcaccga acagcccggc tgttactgct
      661 ggtcatgtaa ccactaacgc actgtacacc attgcttata aataattaat ggtgtgttga
      721 gcataaggtt tgccgccagg cagaccttat tttcgctaat ttaatctttc tacagacagc
      781 aaggagtgct tatgtttccc ttggttaaaa aaacggttag tgcattattt gtaagtactc
      841 tgttagccag tgcgcctgca ttcgctgaca ttattatttc cggaactcgt atcatttata
      901 acgctgataa aaaagatgtt aacgttcgtc tggaaaataa agggaatcgc cccttactga
      961 ttcagaactg gttggatact ggcgacgaca atgccgatcc cagccagatc aaagtgccat
     1021 ttacctctac gccgcctgtt tcacgcgttg agccaaaacg cggtcaaacc gtaaaagtga
     1081 tgtacaccgg tgcaacagcc ctgcctaaag accgggaaag tgtctactgg tttaacgtgc
     1141 tggaagtgcc gcctaagccg aaagatgctg agtctgacaa aaacttatta caattggctt
     1201 tccgcacacg catcaaactc ttttatcgcc cgagcggttt acagggagaa ccggctgaag
     1261 caccggcgaa aatcacctgg aaattaaaca acgctcagtt acaggcaaat aacccaacgc
     1321 catattacgt gtcctttaat gaggtcaagc tggaaagcgg cggcaaaacc tacaacgtca
     1381 attccagcat ggtcgcgccg ttcagccagg caagctttcg cgtaaccggt ctaccaggaa
     1441 gcgtttcatc cgggaaagtg atctttaaag cgatcaacga ctttggcggc aacatcgatg
     1501 gttccgcaac gttctaaaaa ataaacggat tctttttaac atcgccagga atgcaacctt
     1561 gtaccagttt actcatcaaa taagccgtat cccgaaaaaa acgctacttg cggcctgttg
     1621 tgccctgttt tatagcagca acggtgctgc ggcagacacg gtggaatatg acagttcctt
     1681 cttaatggga actggcgcat caacgattga tgttaaacgt tatgctcaag gcaacccgac
     1741 acccccgggc ctctataatg tccgcgtgtt tgtaaacggt caggcgacgt ccagcttaga
     1801 aattccgttt gtggatattg gcgaagccag cgcagcggcc tgtattaccc acaaaaacct
     1861 ggcgcaactc cacattaagc aacctgaaca gcctgtcact ttactcgcca gagaaggtga
     1921 agaagaggat tgtctggatt tggcaaagtc atacgaaaag gcggatgtgc gctttgacgg
     1981 cagtgaccag tttctcgatc tgactattcc tcaggcctat gttctgaaaa actacggtgg
     2041 ttatgtcgac ccctcactgt gggagtcagg gattaacgct gccacactgg catataccct
     2101 gaacgcctat catacaagtt caaataacga caatagtgac agcgcctatg gcgcatttaa
     2161 ctcaggtatc aatttaggag cctggcactt ccgtgcgcgc ggtaactata actggacaac
     2221 agataacggc agcgatttcg atttccagga tcgttactta cagcgtgaca ttccggcaat
     2281 ccgttcacag ataattatgg gtgatgccta taccaccggt gaaacatttg actctgtcaa
     2341 cgtccgcggc gttcgcctgt acagcgatag ccgtatgctg ccttcggcgc tggccagtta
     2401 cgctccgacc atccgcggtg tagcaaactc caacgccaaa gtcacggtga cgcaaagcgg
     2461 ttataaaatc tatgaaacca ccgtaccacc cggtgaattt gttatagacg acattagccc
     2521 ctccggcttt ggtagcgaac tggtcgtgac cattgaagaa gcggatggtt ccaaacgcac
     2581 ctttacgcaa cccttctctt cggttgtaca aatgcaacgt cctggtgtgg gccgttggga
     2641 tttcagcgcg ggtaaagtca ttgatgacag tctgcgatcc gaacccaata tgggacaagc
     2701 ctcttattac tatggtctga ataacctctt cacgggttat accggtattc agttcaccga
     2761 taataactat cttgccgggc tgttaggtgt gggtatcaac accagcatcg gtgcctttgc
     2821 gctagacgtt acccattccc gtgctgaaat tccggatgat aaaacctacc aggggcaaag
     2881 ttatcgcgtg acctggaaca aattattcca ggataccggg acatcattta acctcgcggc
     2941 gtaccgctat tccacccaag attacctggg cctgcatgat gcgttagtcc tcattgacga
     3001 cgccaaacat ttgtctgccg atgaagacaa aaacaccatg cagacgtact cacgtatgaa
     3061 aaaccagttt accgtcagca tcaaccagcc gctgaaaacg acttacgaag attacggttc
     3121 actgtttatt tctggtagct ggacggatta ctgggcggcg aacaatagcc gcactgaata
     3181 taatgttggt tacagtaaaa gcgtttcgtg gggcagtttc agcgtcaacc tgcaacgtag
     3241 ctggaatgaa gacggcgaga aagatgacgc gatgtacgtc agcgttagcg tacctattga
     3301 gaatatttta ggtggcaaac gtaagtcttc tggtttccgc aatttaaata ctcagctcaa
     3361 taccgatttc gatggttcac atcagttgaa tgttaacagt tccggtaaca ctgaaaacaa
     3421 tctggtgaac tacagtgtca acgcaggtta tagcctcgat aaaaactctg gcgatttagc
     3481 ctctgttggt ggttatctca actatgaatc agggttaggc ggtatttccg cttcggcctc
     3541 ggccacttcg gataacagcc aacagtactc catctccacc gatggaggct ttgtattaca
     3601 cagtggtggt ttaacgttca ctaacaacag tttcagcagt aacgacacgc tggtgttaat
     3661 caacgcccca ggtgctaaag gcgcacgaat caataacagt aataacgaaa tcgatcgctg
     3721 gggatatgcc gtgacgtcct ctgtcagccc ctaccgtgaa aaccgggtag gtctgaacat
     3781 tgaaacactg gaaaacgatg ttgaactgaa aagtaccagc gccaccaccg taccacgtag
     3841 cggctccgtt gttttgaccc ggttcgaaac tgacgagggg cgttctgccg tgctgaatat
     3901 tactgccgcc aatggcaaat ctattccgtt tgctgcggag gtttaccagg atgacgtgat
     3961 gatcggcagc atgggccagg gtggtcaggc atttgtgcgc ggtattaacg acagcgggga
     4021 attaatcgtg cgttggtatg aaaacaacca aaccattgac tgtaagttgc actaccagtt
     4081 cccggcccag ccacaaacgc tgggaagtac caacacctta ttacttaata atcttacctg
     4141 tcaggtagca aatcactaat atggaaaatt ctatgaaaag gatactacta acatccgcgt
     4201 taataggcct gggtttaccg gcggttggct ctgcaacaga tcttaatgtc gattttacgg
     4261 ccaccgtatt agccaccacc tgcacgataa ctatcgtaca agatggtggc ccagtagtga
     4321 cgggtagtaa cggtgaatat agcctgacaa ttcccgatgt tggcctggat aaagttataa
     4381 ctggagatgt tgcagctcaa gccaacttta aattactggc ttccgattgc agcaatggtt
     4441 actcaaagat ttttaccaca cttacaggtg catctgtttc aggtaagttg attgtcaatg
     4501 aagcaagctc tggcgctact ggtgtgggta tggggattaa gcgtagagat accgcagact
     4561 caactttctt tacacccaat aatactgaca aatttgaatg gaccaccgat gagaaagcta
     4621 acggtgtgcc tctaaccgta gcactgcgcg aaacaacggc gggagcagga cgcaccggtg
     4681 cgtttcaggc aaaagccacc tttaacttca cctacgaata acagggacag ccaccatgaa
     4741 attaaaagtc atcgctacac tgattgccac tgttgccgtg ggtgccagcg ttagcaacaa
     4801 tctcgcttat gcgggtacga catccgcctc tttaaccgta aacagtaacc tgactatggg
     4861 tacctgtagt gcgaagatac tggataccag tgataccgtg accgataccg ttgcctttaa
     4921 caatgtttat atttctgaac tggcagccaa aagcaaggta caaccattta aaatccgttt
     4981 cagcgattgc gccggtcttc caggaaaaag tgctcaactc gtattggcac ccaaaggtat
     5041 cggttgcgct ggcggcatgt ctaacactgc cggctttgca aataaactta actccaccac
     5101 cgaaggtggt tcaagcggag cagctgtcga agtatggacg actacgaccc ccgggggaag
     5161 tggcagtaag caattcagtt gcattcagcg aaacgtcgtc aatgtcgatc tttctaatgc
     5221 cagcacaaca cagccttatg atttcgattt aagcgcgcgg atgattctcg ctgaagggag
     5281 aacggttaat gatgcattac cgggtagttt cctctctcct acgactttca caatcactta
     5341 tcagtaatag cggataagga tattagaatg ctatgcaggc atcataaatt tgtacgcttt
     5401 ttggggctgg taacagccct cgttacgcct tttgcctatg ctggccagga tgtgaatctc
     5461 accgcgcaga ttgtggccag tacctgtcag gttgaagtca gtaataatgg cgttgtcgat
     5521 ctcggcacag tgacgctgga ctattttgct gacaatgtga ccccgacaat ggattatgcg
     5581 ggtggcaaaa cgtttaatgt taatgtggtc tcttgcgata acattcaaac gacacagtcg
     5641 caaatgaaat tagattttca gccgcaggcg ggatcgcttg cgcaggtgaa taatcaaatt
     5701 tttagtaatg aatatgaaca gcaggctacc ggggcgaaaa acgtcggcat tgtgatattt
     5761 tcggcccagc cgaatcagca gacatttaac gtgcgaggaa cggatggttc ctcaacagca
     5821 atttattcgg tcgcgcctgg caatgctgca ccctcaacct ggacctttta ttcccgtatg
     5881 cagcgggtga ataatgccct tcctcctgaa tcgggaatgg tgagaagcca ggttattgtt
     5941 aatgtatctt atgaataata aggataatta tgcaaaggaa aggcaataaa ctgttaattc
     6001 agttatgcag tgcgatactg ctttttttta ccacatcctg gtatgcattg gcgactgaat
     6061 gttatataga gagcaatgct gcaggggctt atgatattgt tataaaccca actcccttca
     6121 gtatacagtc agcgatggtc acagtcccgg aagaaataag cttcgcgacc tgggatgttc
     6181 atattgatct tcggggcgat aagattggtt gtaaatcctt aggtagtaac acaagcgcgg
     6241 tacattttct taatactgct gatgccagta acatatctac ctacactgac agtcacggta
     6301 atggtttatt gaaaacaacc gttacgggta ttgtttattc agtagaatta caatgtagaa
     6361 attgtggagc cgcggaggat cttagtttga tgcttccagc acaaagcggt ggagataatc
     6421 atacaagcaa ctctgataca aagtgggcct atgagtatag tgacgatagc tggtatttac
     6481 gatttcgttt tttctggacc ccggaatata aaccccaaaa tggggtttca gaaggatttg
     6541 cgataccagg taaaatagcc tcctggtata taggcaatga gaatcaacca tggataaatt
     6601 ttttcgttga tacaagctcc ctgcattttt acgtggagga acccacctgt caaacagttg
     6661 ccctggcaca agatcagggt aatgtcagcg gcaaccaggt aacgcttggg aacaactatg
     6721 tttcggaagt gaaaaatggg cttacgcggg aaattccttt tgccatccgc gctgaatact
     6781 gttatgctag taagattacg gttaagttga aagcggcaaa taagcccagc gacgccactc
     6841 tggtaggtaa aacaacgggc tctgcttcag gcgtggctgt aaaagtgcat tcaacttacg
     6901 aagacagcaa agtattgtta aaagcagatg gtagcaacac ggttgactac aacttcgcaa
     6961 tctggtcaaa caacctgctg aatttgccct ttacggcaca gttggtaccg gatggcagtg
     7021 gtaatgcagt cggtgttgga acattttcag gaaacgcgac cttctccttt acctacgaat
     7081 aagtaaaacg tcattccctc ctcatcgcac cagagttctg ctctggtgcg aacgttttcc
     7141 cccacgaaaa gccattgccc ccttgttttc ggctgttaga atgcgatcaa tctgacttta
     7201 gccatcagga tacgttatga aaccgaccac tattgcttca ctacagaaat ataagcagga
     7261 aaaaaaacgc ttcgccacca tcaccgccta tgactacagc ttcgccaaac tgtttgctga
     7321 tgaagggctg aatgtgatgc tggtgggcga ctcgctgggc atgacggttc aggggcacga
     7381 ctccaccctg ccagttaccg ttgctgatat cgcctaccac actgccgccg tacgtcgcgg
     7441 cgcaccaaac tgcctgttgt tggctgacct gccgtttatg gcgtatgcca cgccggaaca
     7501 agcctttgaa aacgccgcaa cggttatgcg tgccggtgct aacatggtca aaattgaagg
     7561 cggtgagtgg ctggtcgaaa ccgtaaaaat gttgaccgaa cgtgccgttc ctgtatgtgg
     7621 tcacttaggt ttaaccccac agtcagtgaa tattttcggt ggctacaaag ttcaggggcg
     7681 cggcgatgaa gcgagcgatc gactgcttag cgatgcatta gccttagaag ccgctggtgc
     7741 acagttgctg gtgctggaat gcgtgccggt tgaactggca aaacgtatta ccgaatcact
     7801 ggtgatcccg gttattggca ttggcgcagg caacgtcact gacgggcaga tcctcgtgat
     7861 gcacgatgcg ttcggcatta ccggcggcca tatccctaaa tttgccaaaa atttcctcgc
     7921 tgaaacgggc gacatccgcg cggctgtgcg gcagtatatg gctgaagtgg agtccggcgt
     7981 ttatccgggc gaagaacaca gtttccatta aggagtgacg ttgtgttaat tatcgaaacc
     8041 ctgccgctgc tgcgtcagca aattcgccgc ctgcgtatgg aaggcaagcg cgtggcgctg
     8101 gtgcctacta tgggcaacct gcatgatggc cacatgaagc tggtcgacga agccaaagcc
     8161 cgcgccgatg tggtcgtcgt cagtattttc gttaacccga tgcagttcga ccgcccggag
     8221 gacctggctc gttacccacg gaccttgcag gaagactgcg aaaagctgaa caagcgcaac
     8281 gtggatctgg ttttcgcgcc ctcggtaaaa gagatctacc cgaacggtac tgaaacccac
     8341 acctatgttg atgttccagg cctttcgacc atgctggaag gcgcgagccg tccggggcat
     8401 tttcgcggtg tgtcgactat cgtcagcaag ctgttcaacc tggtccagcc ggatatcgcc
     8461 tgcttcggtg aaaaagattt tcagcaactg gcgctgatcc gcaaaatggt tgctgatatg
     8521 ggctttgata ttgagatcgt cggcgtgcca attatgcgcg ccaaagacgg tctggcactg
     8581 agttcccgta acggttatct gacggcagaa caacgcaaaa ttgcgcccgg tctgtacaaa
     8641 gttttaagtt cgattgccga caaattgcag gctggcgaac gggatctcga tgaaattatt
     8701 gccattgcag ggcaagaact gaatgaaaaa ggcttccgcg ccgatgatat tcagattcgc
     8761 gatgccgaca cattgctgga agtctctgaa accagcaaac gggcagtaat tctggtagcc
     8821 gcctggcttg gcgatgctcg cctgatcgac aacaaaatgg tcgaactggc gtaatactta
     8881 actggcgcta cggctgatgg cgccagttat taatttgccc tacgtcatgg cgatgacgtc
     8941 tgtaagtgat tattcatatc gctggcaatt tagttagagt taattagctt atcaatctct
     9001 gtaagaggta aagttgtcac ctctgcaacg tcttcccgag acatcccttt acttagaaaa
     9061 cgctgggcga attcctgtct taccttctgg agttcctctt gatatccctt ctgaaatccc
     9121 ttctcttcaa accactgcgc caacgtctgc ctggtttctc tccgtgtctt tatttccata
     9181 ggttccctta attatctggt cagttaacaa taacaattat ctggctataa ctgatgatgc
     9241 agacaactta gttaatcagc ttatcaatct cggcaagggg taaatttgcc atctctgcaa
     9301 cgtcttcccg agacattcct ttattcagaa gacgcaggac gaattcttgc ctttcctcct
     9361 gtcttcccct ctcttcaaac cactgcgcca gcgtcatcat agactcccct cccgtttccc
     9421 tgtctctcaa cacaccgtaa aacaaatccg cttgttcagt atgaccgcgt tgcagcatat
     9481 agttttgcat ggcaactaac tgacttccgc tagtgtaccc ttcgtcaatc agcgtgacca
     9541 gttgctccag caataacatt aagtcgcgct ggcgaatatg tttttgcagt agttcgagaa
     9601 tcgcaatccg ccgatgttgc atgatttcgt catccggtgt gatggtgata tccaccagcg
     9661 ggaaaggact gttatagacg cgtcgcgcca gctccggcga gtaaaacata tcaaaccagc
     9721 acattgagag cggataaggt gtggcctcgc cctgataaaa gagaatcggc actaccagcg
     9781 gtagtttgtc gtgatccgcc tccagatgcc gatgcatggc ggctatagaa taacgcatca
     9841 tgcgaaaggc cattttctta tccggcttgc tttggtgttc aatcacaaca tgcagataac
     9901 cgggattgcc ctgcatttgc acggaataga gcacgtccgt gctgtgtcct ttaaggctct
     9961 cttcaatgaa actccccgac tctaaatgaa gcgtgttgag gtcacaaagt tcgcgtaact
    10021 ccactggcaa atgtatctcc agaaagtcgc gagccgtctc cgcatgcatt aaaaattgtt
    10081 taaataccgc gtcatgcggt gtggtattcg gtgcatccat cttccctgtc ttccgtgttg
    10141 tcttgcgtga aggcagtatc cagtcaaggc gtgctatcgt cagccacctc tttattgttt
    10201 accgagcagc gttcagagaa atatcaattc gttacaggcg atacatggca cgcttcggcg
    10261 cggtacgcaa actgctgatg caaatgccgt cagatgagta gacactaaac aaaaatcggg
    10321 caatactgcg tgagaatttc ctcaaagcag gcaccgcctg cttcgttaac gacagggtag
    10381 aaaggtagaa gttatgattc gcacgatgct gcagggcaaa ctccaccgcg tgaaagtgac
    10441 tcatgcggac ctgcactatg aaggttcttg cgccattgac caggattttc ttgacgcagc
    10501 cggtattctc gaaaacgaag ccattgatat ctggaatgtc accaacggca agcgtttctc
    10561 cacttatgcc atcgcggcag aacgcggttc gagaattatt tctgttaacg gtgcggcggc
    10621 ccactgcgcc agtgtcggcg atattgtcat catcgccagc ttcgttacca tgccagatga
    10681 agaagctcgc acctggcgac ccaacgtcgc ctattttgaa ggcgacaatg aaatgaaacg
    10741 taccgcgaaa gcgattccgg tacaggttgc ttgattgtta tccctgcggc tggttactca
    10801 ccagccgcga catcgtctcc agcgaatccg ttcttaagat ataaagtcgt tttagtaaca
    10861 acggattatc ccccggtttt actttgcctt tcatggttgt caccgccagg tgaaatccgg
    10921 catcgtttgc cgccttcacg gctttgtcat taaagccgcc aaacggatac gaaagatacc
    10981 agacatgcgg attaaattgc gccagagcgc ggcgtgaacg tgcaaaatca aacagaatat
    11041 tgtgctcact acggctcagt aatatgggtc ggcgataacc atctacccga tgcaaaaaat
    11101 gggtatgtga ctggaaatca aatacatctc gaatttcgtt aagctcagaa acgctcataa
    11161 attgcagcga ttttgggttc cacttctgcg ggtgacgttt gatgcgtgag gtaacaataa
    11221 acgccgtcgc cttcatgcca tattgtttca acacaggata cgcatagcgg ctcaccgact
    11281 tgaggccatc atcaaaggta atcaccaccg ctcgcgcagg gagattgatc ttattcttca
    11341 cgtaaccttc cagctgcacc atgctcagtg tcgcgtatcc cctgtcgcgc agccaggcca
    11401 tctggttatt gaatgcgcgt accgaggtgg tcgtcgaagt atggcgaaaa cgggtgtttt
    11461 cttcgtcgcg cagaatatgg tgataagtta gcaccggaag gccattatcg ggttgggcat
    11521 ccagcgcgct gatataggcc agtcgatcgc caatacggac ctgataccag gtttgattta
    11581 acctgtcttt cagtttatgc aaaatcgggt agcgcaaatt gtccgccagt accccaaatg
    11641 gcgcactgcc cacactcggc gcgttataga ccggcgtatc tttccaggta actaagttct
    11701 gattactcag cggcttgttg aggtcaccca aaccgtcttc aactttttgt cgcccctgaa
    11761 ccggctcgag gtgcccttta tcgataaacc ctttaccaaa gccaaaatta aatgcgtaat
    11821 aacttgcggc agtgggctcc acggcaatga tttgtccggc ccgaatattc cccacggtca
    11881 ccatcttgtc gccaatttgc gcccagaccg cagcattttc gatggtttgc atataacggg
    11941 caggtaacgc ggcactgaca cttgcggtaa acagcatcag caggagaata acagcttgtt
    12001 tgtacatgaa aaaataaccg agggtgaaac aacgctttta ttgtagcaaa agcgccatca
    12061 gaatcgcggg ttaatactca tacaaattat gtttgaggac gaaaggcgga tttttttgtt
    12121 gttggtgcca aagactactc acctccggcg cacccagttc gacaatacgc tcgcgaaact
    12181 ctgaactggt catggtttca cggcaagcca tcatctgttc aattaacggt aacgtgacac
    12241 cagaaatcac ttcgcaacgg gagtgtttgt ggcttaataa tgaagccacg cgatacggtg
    12301 gcgctccggc tatatcggtt aagaagatga caccctcacc agagtccgct tcatgcagag
    12361 catcgcacat catgcggctg agcatattag aacttaatcc gcgccagaaa ttcacggccc
    12421 ggcactgaag aagcgcccca tgttttttct ccagcgcatc cagtatctct tgcgcccggt
    12481 catcgtgaca ggtaattacc caacctaaca tcgccttcct ccttagccga aaatcagtct
    12541 accctgacca ttgttgataa agggtgatag ccgtcaaaac tcctctcccc caaatccaga
    12601 ggagaggaaa tagccttagc tacgcaaacc acgtccacgt tggatcagcg accaacagat
    12661 caaataaaac gccacaataa agaccaccag tacgccaaaa gtagtgacca gcggaacatc
    12721 attgataccg aggaagccgt agcggaaacc actgatcata taaacgattg ggttcaggtg
    12781 cgacagccct tgccagaacg gcggcaacaa agtcagtgag taaaagaccc cacccaaata
    12841 cgtgagtggc gttaacacaa aggttggcac caggctgatg tcatcgaacg ttttggcaaa
    12901 cacaccgttc agcaaacccg caagggagaa caacaccgcc gtgagcacca gggttaaggc
    12961 aacgaatacc cacgaatgca cctgaaacgg cacaaaaaac agtgaaattg ccgtcaccag
    13021 aatgccaaca aacagaccac gcgccacgcc accgccgaca tatccggcaa taatgacgtg
    13081 agtcggaacc ggcgctacca gcagctcttc aatattacgc tggaatttgg caccaaaaaa
    13141 tgatgacgcg acgttagcgt aggcgttggt gatcaccgac atcatgatca gccccggtac
    13201 gatgaactgc atgtagctga agccatgcat atcgccaata cgcgaaccaa tcaggttacc
    13261 gaagataata aagtaaaggg tcatggtgat gactggcggc accagcgtct gcacccagat
    13321 acgcataaag cgatggatct ctttcgccca gatgcttttt agcgccaccc agtaaagatg
    13381 catcatgcgc gatctccttg tttttcatta accagtgaaa caaacagctc ttccagacgg
    13441 ttagctttgt tacgcatact taatacctga atgccctgct cacttaactg cgtaaatacg
    13501 ctgttgatcc cctgctcacg cagcacttca acttccagcg tcgcggtatc gaccagtcga
    13561 tactgatagc catcgagctt cggtaacgga cttttcggtg cgagatcgag aataaaggtt
    13621 tccgatttca gcttcgccag cagcgccttc atcgaggtat tttccaccag ctcaccgtgt
    13681 tgaataatgc cgatattgcg gcacagcatt tctgcttctt ccaggtagtg tgtggtgaga
    13741 atgatggtgg tgcctttgtc gtttaaatcc ttcaaaaagc cccacattga gcggcgaagt
    13801 tcaatatcca cgcctgcagt cggctcgtcg agaatcagta gtttaggttc atgcattaac
    13861 gcacgggcaa tcattaaacg gcgcttcatc ccgccagata acatgcgcgc acgttcgttg
    13921 cgttttcccc ataaatcgag ctgcttaaga tatttttcgc tgcggatgta cgcctctttg
    13981 cgctccacac cgtagtaccc tgcctgattc accacaattt gctgcacggt ttcaaacggg
    14041 ttgaagttaa attcctgcgg caccagtccc aactgacgtt tagcgttcac gacatccttc
    14101 tcgagatcgt aaccaaatac gctgacccgc ccggaggttt tatttaccag agagctgata
    14161 ataccgatag tggtcgattt cccggccccg ttcggcccga gaagcgcata aaaatcaccc
    14221 gcttcgacct gcaaatctat tccacgaagc gcctgaacgc cgcctggata ggttttttta
    14281 agctgttgaa gttccagtgc aatggtcata aatttttact taccttacgt tcttacactt
    14341 tatctgtggt ttaaatcgtc ccggagttgc cctatattag ccaaacgtaa ttatttggtt
    14401 acaggtcgtt aacctccatg aaagacatag atacactcat cagcaacaat gcactatggt
    14461 caaaaatgct ggtggaagag gatcccgggt tttttgagaa actggcacaa gcgcaaaagc
    14521 cgcgctttct atggattgga tgttccgaca gtcgcgttcc tgcagaacgt ttaaccggtc
    14581 ttgagccggg cgaactcttt gttcaccgta atgttgctaa cctggtcatt cacactgacc
    14641 tgaactgcct ttccgtggtt cagtatgcag tggatgtact cgaagttgaa cacattatta
    14701 tctgtggcca ctacggttgc ggcggcgtac aagccgccgt tgaaaacccg gaactggggc
    14761 ttatcaacaa ctggctgctg catatccgcg atatctggtt caaacatagc tcattgctcg
    14821 gcgaaatgcc gcaagagcgc cgtctggata ccttgtgtga actgaacgtc atggaacagg
    14881 tgtataacct gggccactcc accattatgc aatcagcgtg gaaacgcggg cagaaagtta
    14941 ccattcacgg ctgggcctac ggtattcacg acggcttgct gcgtgatctg gatgttactg
    15001 ccaccaaccg cgaaaccctt gagcaacgtt accgtcacgg gatttccaac ctcaagctga
    15061 aacacgccaa ccacaaataa aaatgccgtg ccggatgcaa cacatccggc aacttcacac
    15121 ttactcgtcc agcagaatca ctttgccaat atacggcaga tgacggtaac gctgtgcgta
    15181 atcaatgccg taacccacca caaactcatc cgggatcgag aaaccgataa attctaccgg
    15241 gacgttcact tcacgacggg acggtttatc cagcagcgta caaatcgcca gcgacttcgg
    15301 ttcgcgcagg cttaagatct cacgcacttt cgacagtgta ttccccgagt cgatgatatc
    15361 ttcaacaatc agcacgtcct tgccacggat atcttcatcc agatctttga ggattttcac
    15421 atcacgggtg gtggacatgc cgctaccgta gctggaggcg gtcataaagt cgacttcatg
    15481 agatacctga acttcacggc acaggtccgc cataaacata aatgagccac gcagcagacc
    15541 caccagcacc atatcgctgc cgctgtcttt gtaacgctca gtaatctgac gacccagttc
    15601 ggcgatacgc gctttaatct ccgcttcggg gatcattact tctacagtat gtttcatatc
    15661 tctaaccata tgatttaaaa acaaatcact tcgcacgggc aggttttggc accgcaacaa
    15721 agcgcaagac acgcagtata ccagtaaaac tattcattcg cggaatctgt taataaagct
    15781 cgaattgtga tgacggtcac acatgttaaa acctataatt aattgctact aaaatattaa
    15841 tgaattgaaa tggtgtctct ttatggcaat taacaataca ggctcgcgac gattactcgt
    15901 cacgctaaca gccctttttg cagcgctttg cgggctgtat ctactcattg gcggaggctg
    15961 gctggtcgcg attggcggct cctggtacta ccctatcgct ggccttgtga tgctcggcgt
    16021 cgcctggatg ctgtggcgca gtaaacgcgc cgcgctttgg ctatacgcag ccctgctgct
    16081 cggcaccatg atttggggcg tctgggaagt tggtttcgac ttctgggcgc tgactccgcg
    16141 cagcgatatt ctggtcttct tcggcatctg gctgatcctg ccgtttgtct ggcgtcgcct
    16201 ggtcattcct gccaacggcg cagttgccgc actggtggtg gcactgctga ttagcggtag
    16261 tatcctgacc tgggccggat ttaacgatcc gcaggaaatc aacggcacct taagcgccga
    16321 tgccacacct gcggaagcta tctcacccgt tgccgatcag gactggcctg cctatggtcg
    16381 caatcaggaa ggtcagcgct tttcacctct gaatcaaatt aacgccgata acgtccataa
    16441 tctgaaagaa gcctgggtgt tccgtactgg cgatgtgaag cagccgaacg atccgggcga
    16501 aatcaccaat gaagtgacgc cgattaaagt gggcgacacc ctttacctgt gtaccgctca
    16561 ccagcgcctg tttgcgctcg atgccgccag cggcaaagag aaatggcatt acgatcctga
    16621 gctgaaaact aacgagtctt tccagcacgt aacctgccgt ggcgtctctt atcatgaagc
    16681 caaagcagaa acggcttcgc cggaagtgat ggcggattgc ccgcgtcgta tcattcttcc
    16741 ggtcaatgat ggtcgactga ttgcgattaa cgctgaaaac ggcaaactgt gcgaaacctt
    16801 cgccaataaa ggcgtgctca atctgcaaag caatatgcca gacaccaaac cgggtctgta
    16861 tgaaccgact tcgccaccga ttatcaccga taaaaccatc gtgatggcag gttcagttac
    16921 cgataacttc tcaacccgcg aaacgtctgg cgtgatccgt ggttttgatg tcaacaccgg
    16981 ggagctgctg tgggcttttg atccgggcgc gaaagatccg aacgcaatcc cgtctgacga
    17041 acacaccttt acctttaact cgccaaactc gtgggcacca gcggcctatg acgcgaagct
    17101 ggatctggtg tatctgccga tgggcgtgac cacgccggat atttggggcg gtaaccgcac
    17161 accggaacag gaacgttatg ccagctcgat tctggcgctg aatgccacaa ccgggaaatt
    17221 ggcgtggagc taccagaccg ttcaccacga cctgtgggat atggatcttc cggcacagcc
    17281 gacgctggcg gacatcaccg ttaatggtca gaaagtgcca gttatttacg ctccggcgaa
    17341 aaccggcaac atttttgtgc tcgatcgtcg taatggcgaa ctggtggttc cggcaccgga
    17401 aaaaccggtt ccccaaggtg ctgccaaagg cgattacgtt acccctactc aaccattctc
    17461 ggaactgagc ttccgtccga cgaaagattt gagcggtgcg gatatgtggg gagccaccat
    17521 gtttgaccag ctggtgtgcc gcgtgatgtt ccaccagatg cgctatgaag gcattttcac
    17581 gccgccatct gaacagggca cgctggtctt cccgggtaac ctgggaatgt ttgaatgggg
    17641 cgggatttcc gttgatccaa atcgtgaagt ggcaattgcc aacccaatgg cactgccgtt
    17701 tgtttcgaaa ctgatcccac gcggtccggg taacccgatg gagcagccga aagatgccaa
    17761 aggcacgggt acggaatccg gcattcagcc acagtacggt gtaccgtatg gtgtcacgct
    17821 caacccgttc ctctcaccat ttggtctgcc atgtaaacag ccagcatggg gttatatctc
    17881 ggcgctggat ctgaaaacta atgaagtggt gtggaagaaa cgtattggta cgccgcagga
    17941 cagtatgccg ttcccgatgc ctgtaccggt gccgttcaat atgggtatgc cgatgctggg
    18001 cgggccaatc tccacggcgg gtaacgtgct gtttatcgcc gctacggcag ataactacct
    18061 gcgcgcttac aacatgagca acggtgaaaa actgtggcag ggtcgtttac cagcgggtgg
    18121 tcaggcgacg ccaatgacct atgaagtgaa tggtaagcag tatgtggtga tctccgcagg
    18181 cggtcacggt tcatttggta cgaagatggg cgactatatt gtggcttatg ccctgccgga
    18241 tgatgtgaag taagacttgc tcagattgct gacaacgtgc gcgttgttca tgccggatgc
    18301 ggcgtgaacg ccttatccag cctacaaaat tgtgcaaaat caatggattg cacaggtagc
    18361 gtaggcctga taagcgtagc gcatcaggca gatttgcgtt tgtcatcagt ttaatgcccg
    18421 gagagatccg ggcatatttc cgaatacggt ctttttatac cgtaaaccct aacatcatcc
    18481 ccgtatcttc atgctccagc agatggcagt gcgccatata agcatgttct ttcggtgcat
    18541 cgtgattaaa cttcaccagc acttcgctga cattaccttc taccttaacg gtatctttcc
    18601 agcccgcgcg atgagccgct ggcggtttgc cattttctga caagatacgg aactgcgtgc
    18661 cgtgaatatg gaacggatgc agcatcatgt cacccacgcc agagataacc caacgttcat
    18721 actgcccttt cgccgccgca aacatcggct tattcatatc aaacgcctga ccgttgattt
    18781 tattggcatg gtggaaatcg aacttcccgc cgtggttcat atgattcata ttgccgtgcc
    18841 ccatatggcc catcatctgg ctgtgatcca ttccggccat cgcctgatcg ccatatttct
    18901 ccattagcat ctgcatcccc atcatatcga gcatcgggtc catagaaagt tgcagcttgc
    18961 gtaccgtcag cccttccagc gaaggtaacg caggcaggct acttaatgtg tctggcaaag
    19021 caccggaggc actaatagca atcggctgaa tccgcattac cggatgcggc ttgtcaaacg
    19081 gtgcaatcgc catccccatc tggctgaccg gcagcgtcac caggtcaaag ggtttgttgt
    19141 cgttaacctc caccagcact tcaaaacgct cgcccatcag caccggcagc tcgttcacct
    19201 tcaccggttc aggtagcaga ccaccgtcgc tggcaatcac ataaagcggg cgattgtcgc
    19261 tggtggcgaa attgagcgag cgggcattac agccattgag caaacgcagg cgcagccaac
    19321 cacgcggggc agcgtgttgc gggtagattg caccgttggt cagcaacgta tcgccaaacc
    19381 agcccacggc ggcggtcatc acatccagtt gataatcaat ctgcccgtcg gcgctaaatt
    19441 tcttatcctg aacgatcacc ggaacatcat cgatacccca ctgttttggc agcattaatt
    19501 tcaggatctc gtcatcttca atcaccacca gaccagccag ccccatcgcc acctgtcgcc
    19561 cggtcttgcc atgttgatgt ggatggaacc agcaggtagc ggcaggttga tcaacgttca
    19621 acgtcaccga gcgcttgcca cctggcggaa taattccctg tgggccgccg tcgacttcac
    19681 ccggtacttc cagcccgtgc cagtgcaacg tcgtctcttc cgtcagttgg ttatagatat
    19741 caaccgttac cgctttgccg cgctgtaatt tcaccgccgg ccccaacaga ttgccgttat
    19801 agccccaggt agttgcagtt ttctcgccaa aggtagactg acctgcgcca atagttaact
    19861 gaatgcgatt acgggcatcg gtcgtgagca aatcagggat cggtaacgtt gggcgttccg
    19921 ccgcaaagac tgcgcggctc cacagcggca aggctgaagc cacacccagc gcgacggaat
    19981 atttcaagaa atcacgacgt tgcatagtta tttccttatt cttaagcagg cgaaacaaaa
    20041 tcaaacgttg agcatagtcc ttccccttac gggaaggtca agccgcagac acaatttaat
    20101 cgacaataat ggtcgtcgcc tcgctcgcag tgtgctaacg tttatcttct ttaagcccct
    20161 ggtagaagca atgaagacgt ttttcagaac agtgttattc ggcagcctga tggccgtttg
    20221 cgcaaacagt tacgcgctca gcgagtctga agccgaagat atggccgatt taacggcagt
    20281 ttttgttttt ctgaagaacg attgtggtta ccagaactta cctaacggac aaattcgtcg
    20341 cgcactggtc tttttcgccc agcaaaacca gtgggatctc agcaactacg acaccttcga
    20401 catgaaatcc cttggtgaag acagctaccg cgatctcagc ggtatcggca ttcccgtcgc
    20461 taaaaagtgc aaagccctgg cccgcgattc cttaagcctg cttgcctacg tcaaataatc
    20521 cctgatacct ttttgtagaa atattgaccg tgcatccgcg gtcaatgtta gctattatgt
    20581 tgcgcccttt ttttacgggt gttaacaaag gaggtatcaa cccatggccg aaaaaaaaca
    20641 gtggcatgaa acgctacacg accagtttgg gcagtacttt gcagtagata acgttctgta
    20701 tcatgaaaag accgatcacc aggatctgat catttttgag aacgctgcat ttggtcgcgt
    20761 aatggcgctg gatggcgtag tacaaaccac cgagcgcgac gagtttatct atcatgagat
    20821 gatgacccat gttccgctac tggcccacgg tcacgcgaaa catgtgctga ttatcggcgg
    20881 cggcgacggt gccatgctgc gtgaagtaac ccgacataaa aacgttgagt caatcacgat
    20941 ggtggaaatc gatgcgggtg tcgtgtcgtt ctgccgtcag tatctaccca accataacgc
    21001 cggtagctac gacgatccgc gctttaagct ggtgatcgac gatggcgtca atttcgttaa
    21061 tcaaaccagc cagacctttg atgtcattat ctccgactgc accgatccta tcggtcccgg
    21121 cgaaagcctt ttcacttcgg cattttatga aggctgcaaa cgttgcctga atcctggcgg
    21181 tatcttcgtc gcacaaaacg gcgtctgctt tttacagcag gaagaagcca tcgacagcca
    21241 tcgcaaactc agccattact tcagcgacgt tggcttttat caggcggcga tcccgaccta
    21301 ttacggcggt atcatgactt ttgcatgggc gacagataac gacgccttac gccatctctc
    21361 aaccgaaatt attcaggcgc gttttctcgc ctctggcctg aaatgccgtt attacaatcc
    21421 ggcaatccat acggcagctt ttgccttacc tcagtatctg caagacgcac tggcttcaca
    21481 gccgtcctaa ggagaagata agaaattgaa aaaactgaaa ctgcatggct ttaataatct
    21541 gaccaaaagt ctgagttttt gtatttacga tatctgctac gccaaaactg ccgaagagcg
    21601 cgacggttat attgcttata tcgatgaact ctataatgcc aaccgtctga ccgaaatcct
    21661 gtcagaaacc tgttccatta tcggggctaa tattcttaac atcgcccgcc aggattacga
    21721 accacagggt gccagcgtca ctattctggt gagtgaagaa ccggttgacc cgaaactcat
    21781 cgacaaaaca gaacaccccg gcccactgcc agaaacggtc gttgcccatc tcgataaaag
    21841 tcatatttgc gtacatacct acccggaaag tcatcctgaa ggcggtttat gtaccttccg
    21901 cgccgatatt gaagtctcta cctgcggcgt gatttctccg ctgaaggcgc tgaattacct
    21961 gatccaccag cttgagtccg atatcgtaac cattgattat cgcgtgcgcg gttttacccg
    22021 cgacattaac ggtatgaagc actttatcga ccatgagatt aattcgattc agaactttat
    22081 gtctgacgat atgaaggcgc tgtatgacat ggtggatgta aacgtctatc aggaaaatat
    22141 cttccatacc aagatgttgc ttaaagagtt cgaccttaag cactacatgt tccacaccaa
    22201 accggaagat ttaaccgaca gcgagcgcca ggaaattacc gctgcgctgt ggaaagaaat
    22261 gcgcgagatt tattacgggc gcaatatgcc agctgtttaa cggctctggc ggagctccca
    22321 ggctccgcca gatttattta cttctgctgc acgaaattgc ggtaagccgc cacgacctgc
    22381 agaaaatcct caacgccgca tagcgacagg ctttcttcgt cgtagtagtt catcccctct
    22441 tccatttcat cgccagcgaa ttccagttga ttggcgcgaa ccatcacctc ttcaccgtcc
    22501 atccacaggg tgtattcatg ccctgcccgt tgccaggacc gttcgctacc tttcagtgcg
    22561 tgcgctgctt gttccacttc atcaagcaag gccaggtttt ctttcacctc ttcattaaac
    22621 cagtgcccga ccacttcatg ccccatggac atacgcacct ttaccactcc ggtaatatcg
    22681 cgcagaaatt cgtaatccat agtgtgttcc tctgctcccg gcaatgcacc gaaatcgcat
    22741 tgcgccattg atgtaattat cgcagcctta ccggagaaaa acagcggaac ggcgatggtt
    22801 tagaaataaa aaaatgcccg ggaatctcat tcaccgggca ttgtgtcgtt tatgcgcagc
    22861 gcgtgcgctg actttttaaa ccgcagtctg gaaaatcacc ccatcggctt tctcggtgta
    22921 ctgagaaagc tggttgaagt tcagataacg gtaagtatca acggctgttt tatctacctg
    22981 cgccacgtag gtctggtact cttccggcgt cggcagtttg ccaatcagcg ccgcaacagc
    23041 agccagttcc gcagaagcca ggaagacatt cgcgccagta cccagacggt tcgggaagtt
    23101 acgggtagag gtggaaacca ccgttgcacc gtccgccaca cgcgcctggt tacccataca
    23161 cagggaacag ccagggatct cgatacgcgc accactctta ccgaagacgc tgtagtagcc
    23221 ttcttcggtc aactgtgcgg cgtccatacg ggttggcggt gccacccaca ggcgggtcgg
    23281 caactggcct ttatgcgcat ccagcagttt acccgcagca cggaagtgac cgatgttggt
    23341 catgcaggaa ccgataaaca cttcgtcgat cttctcaccc tgtaccgcag acagcggacg
    23401 cgcgtcatcc gggtcgttcg gagcacacag gattggctct ttaatatccg ccagatcgat
    23461 gtcgatcact gccgcgtatt ccgcatctgc atcggcttcc agcagctcag gattcgccag
    23521 ccatttttcc atgccctgaa tacgacgttc cagggtacga cgatcgccat aaccttccgc
    23581 gatcatccac ttcagcagga cgatgttaga gttcaggtat tcgatgatcg gttctttgtt
    23641 cagcttgata gtacaaccag cggcagaacg ctcagcggac gcatcggtca gttcaaatgc
    23701 ctgctcaact ttcagatccg gcagaccttc aatttccaga atgcgaccag agaagatgtt
    23761 tttcttgcct ttcttctcaa cggtcagcag accttgtttg atcgcataca gcgggatcgc
    23821 gtgtaccaga tcgcgcaggg tgatgcccgg ctgcattttg cctttgaagc gcaccagaac
    23881 ggattccggc atatcaagcg gcattacgcc agttgcggca gcaaacgcca ccagaccaga
    23941 acccgccggg aaagagatac cgatcgggaa acgggtatgg gagtcaccac cggtaccgac
    24001 ggtatccggc agcagcatac ggttcagcca ggagtgaatg acgccgtcac ccggacgcag
    24061 cgacacaccg ccacggttca taatgaagtc cggcagcgtg tggtgcgtgt tcacgtcaac
    24121 tggcttcgga tacgccgcgg tgtggcagaa agactgcatc accaggtcag ccgagaagcc
    24181 caggcacgcc aggtctttca gttcatcacg ggtcatcggg ccggtggtgt cctgggaacc
    24241 tacagaagtc attttcggtt cacagtacgc gcccggacga atgcctttca cgccacaggc
    24301 acggcctacc attttttgcg ccagcgagaa gccgcgatcg ctctcagcga catctttcgc
    24361 ctgacggaac acatcactgt gcggcagacc aagtgcttca cgcgctttgg tagtcaggcc
    24421 acgcccgata atcagcggaa tacggccgcc agcacgcact tcatcaatca gcacgtcggt
    24481 tttcagttcg aaggttgcca gcaactcgtt ggtttcgtgg ttacgcactt cacctttgta
    24541 cgggtaaacg tcaattacgt cgcccatgtt caggttagag acgtcgactt cgattggcag
    24601 tgcacccgcg tcttccatcg tgttaaagaa gattggtgca attttaccgc cgaggcacaa
    24661 accaccgccg cgtttgttcg gcacatgtgg aatatcatca cccataaacc acagtacgga
    24721 gttcgtggcg gatttacgcg atgaacccgt acccacaacg tcaccgacgt acgccagcgg
    24781 gaaacctttc tgttgcagag cttcgatttg cttgatcgga ccaacaacac caggctgatc
    24841 tggctcaata ccttcacggg cgtttttcag catcgccagc gcgtgcagtg ggatatccgg
    24901 gcgtgaccac gcatccggtg ccggagaaag gtcatcggtg ttagtttcgc cagtgacttt
    24961 gaagacggta acggtcagtt tttcagccag cgccgggcga ttaaggaacc attcggcatc
    25021 cgcccaggac tgcattacct gcttcgcata ttcgttgcct gctttcgctt tctcttctac
    25081 gtcatagaag ttatcgaaca tcagcagtgt gtgagaaagt gctttggcag cgatcggtgc
    25141 cagtttggca tcatccagcg cgtcgatcag cggatgaatg ttgtaaccac cctgcatggt
    25201 gcccagcagt tcgatggctt tttccggagt cagcagaggg gatttggctt cgcctttcgc
    25261 gatagcagcc aggaagcctg ctttgacata ggcggcttca tcgacgccag ggggaacacg
    25321 gttggttaac agatctaaca ggaattcttc ttcgcccgcg ggcgggtttt tcagcagctc
    25381 tacaagtgcg gccatttggt ttgcatccag gggtttgggc gcaatcccct cagcggcacg
    25441 ctcagctacg tgcttacggt attcttctag cacgacggtt ctcctcgctc tcattgtcat
    25501 agtgcggcag gcgattctct tcacgctcct gtgagacagc agtttgtagg gtaaatgccc
    25561 agtaccgcaa cgggcagaat agcaggattt tgacgatgtg ttaattcgtt tacaaaaaag
    25621 caacacaaaa aagtggctga atcgttaagg atggtctaga gattgtttcc ctccgtaaaa
    25681 acttcgttaa ttcgcatggt gataatcacc tttctcaacg aatcccaaga ttagtcaaaa
    25741 tttaaactac cgcctcttta tactcggatt cacagcacct gcgggtggca gttcgccgac
    25801 cattgcgatt tccttgagat ccgaattatg aaaatgactt tgccgtttaa accccatgtg
    25861 ctggcgctaa tttgcagtgc cgggctttgt gccgcctctg ccgggctata tataaaaagc
    25921 cgcacagtgg aagcgcctgt ggaaccgcaa tcgacacaac tggctgtgtc tgacgctgcc
    25981 gcggttacgc ttcctgcaac ggtttccgca cctcccgtaa cacccgccgt cgttaaatcc
    26041 gcattcagca ctgctcaaat agatcaatgg gttgcacctg tcgcgctata ccccgactct
    26101 ctgctttcac aagtgttaat ggcatcaacc tatccggcaa acgttgctca agcagtgcaa
    26161 tggtcacacg ataatccact taaacaaggc gatgctgcta ttcaggcggt atctggccag
    26221 ccgtgggacg ccagcgtcaa atcactggtg gcctttccac aattgatggc attgatgggc
    26281 gaaaacccgc aatgggtgca aaacctgggc gatgcttttc tggcgcagcc gcaggacgtg
    26341 atggactcgg tacagcgatt gcggcaactg gcgcaacaaa ccggctcgct gaagtcatca
    26401 accgaacaga aagttattac cacaacgaag aaagctgtac cggtaacaca gacagtcacg
    26461 gctcccgtca taccatccaa taccgtttca actgccagcc ccgtcattac agagcctgca
    26521 acaaccgtca tttccattga gcccgccaat cctgaggtgg tctatattcc caactacaac
    26581 ccaaccgtgg tttacgggaa ctgggccaat actgcgtacc cgccggttta tctgccacca
    26641 ccagccggag aaccgtttgt tgacagcttt gtgcgcggat tcggctatag catgggcatt
    26701 gctaccacgt acgcactatt cagcagcatc gactgggatg acgacgatca tgaccatcat
    26761 catcatgacg atgatgatta tcatcaccac gatggcggtc atcgtgacag taatggctgg
    26821 caacacaacg gcgacaacat caatatcgac gtcaacaatt tcaaccgtat caccggtgag
    26881 catcttactg ataagaatat ggcatggcgg cacaatccaa actaccgtaa tggtgtgccc
    26941 tatcatgatc aggatatggc aaagcggttt catcaaaccg atgtcaacgg cggaatgagt
    27001 gccacgcagc tacctgctcc aacacgcgac agccagcgtc aggcggcagc aagtcagttt
    27061 cagcaacgaa cacacgccgc ccccgtcatt acacgagata cccaacgtca ggcagcggca
    27121 cagcggttta atgaagctga aaactatggg agctatgacg acttccgcga cttcagccgt
    27181 cgccaaccac tgacccagca acaaaaggac gccgctcgtc agcgttatca gtcggcctct
    27241 cctgagcagc gccaggcagt ccgcgagaaa atgcagacta acccacagaa ccagcagcga
    27301 agagaggcag cgcgtgagcg tattcagtct gcaactcccg aacagcgcca ggtgtttaag
    27361 gaaaaagtac agcagcgccc actgaaccaa cagcaacgtg ataacgcccg ccagcgtata
    27421 caatcggcat cacctgaaca acgtcaggtt tttcgggaaa aagttcagga gagccgccca
    27481 caacgtctaa acgacagtaa ccatactgtc aggctgaata acgagcaacg gtcagcagta
    27541 cgcgaacgtc tctctgagcg cggagcaagg cgacaggaaa ggtaaattgc agacgtaaaa
    27601 aaagcggcgt ggttagccgc ttttttaatt gccggatgtt ccggcaaacg aacaattact
    27661 tcttcttcgc tttcgggttc ggcaggtcgg taatgctacc ttcgaacact tctgccgcca
    27721 ggcccacaga ctcgtgcaga gtcgggtgcg cgtggatggt cagtgcgatg tcttcagcat
    27781 cacaacccat ttcgattgcc aggccgattt cacccagcag ctcgccgccg ttggtaccga
    27841 caatcgcacc accgatcaca cggtgagatt ctttgtcgaa aatcagcttg gtcataccgt
    27901 ctgcgcagtc ggaagcgata gcacgaccag aagcagccca cgggaaggtg gcggtttcat
    27961 agctgatgcc tttctctttc gcttctttct cagtcagacc tacccatgca acttctggtt
    28021 cggtataggc gatggacggg ataactttcg gatcgaagta gtgtttctta ccggcgataa
    28081 cttcagcggc aacgtgacct tcgtgaacac ctttatgtgc cagcatcggc tgaccgacga
    28141 tatcgccgat agcaaagatg tgcggtacgt tggtacgcag ctgtttgtca acgcggatga
    28201 aaccacggtc gtcaacttcc acgccagctt tgcctgcgtc gaggttttta ccgttcggca
    28261 cacgaccaat cgctaccagc acggcgtcgt aacgctgcgg ttcagcgggt gcttttttgc
    28321 cttccatcgt cacataaata ccgtcttctt tcgcttcaac ggcggtaact ttggtttcca
    28381 gcatcaggtt gaatttcttg ctgatacgct tggtgaagac tttaacgatg tctttgtcag
    28441 ctgccgggat aacctggtcg aacatttcaa ccacgtcaat ctgtgaaccc agcgcgtggt
    28501 atacggtgcc catttccaga ccgatgatac cgccacccat taccagcagg cgttctggta
    28561 cttctttcag ttccagcgcg tcagtggagt cccagatacg cggatcttca tgcggaataa
    28621 acggcagttg aatcgggcga gaacccgctg caatgatcgc gttgtcgaag ttgatcacgg
    28681 ttttaccgtt ctcaccttca acttccaggg tgttagcccc ggtaaattta cccagaccgt
    28741 tgaccacttt gactttgcgg cctttcgcca taccagccag accaccggtc agctgattga
    28801 tcactttctc tttccaggta cgaatcttgt cgatatcggt tttcggttcg ccgaagacga
    28861 taccatgttc agccagcgct ttggcttctt cgataacttt tgctacgtgc agcagtgctt
    28921 tagaagggat acagccgacg ttcaggcaaa caccgccaag ggtgttgtaa cgttctacga
    28981 ttacggtttc cagacctaaa tcagcgcaac ggaaggcagc ggagtaacct gcggggcctg
    29041 ccccaagtac cacgacctga gttttgattt cagtactcat catgacctct atatatttat
    29101 ctccggcggg tctgacgttt tttttatgac gcccattatc caccgggtcg ttctatccgt
    29161 cggtatttta caaaattgtt aacaattttt aaacaacaaa cggcaaccga tttgtctatt
    29221 cgctaataac ctcaatacat tcatgagatt accagaaaaa agccggccgt tgggccggct
    29281 cttttactta catcaccaga cggcgaatgt cagacagcgt gttgttaatg atggtaatga
    29341 aacgggcacc atcagcaccg tcgatcacgc ggtggtcgaa ggagagagaa atcggcagca
    29401 tcagacgcgg cacgaactct ttaccattcc acaccggctc catcgcggac ttggaaacgc
    29461 cgaggatagc cacttccggc gcgttcacaa tcggcgcgaa gtgggtagta cccaggccgc
    29521 cgatgctgga gatggtgaag caaccaccct gcatttcgcc cgcagtcagc ttaccgtcac
    29581 gcgctttctt agaaatagtc atcagctcgc gagacagctc gatgatgcct ttcttgttga
    29641 cgtctttgaa taccggaaca accagaccgt tcggggtatc caccgccaca ccgatgttga
    29701 tgtatttctt cagggtcaga cgctgaccgt cttccgacag cgaactgttg aagcgaggca
    29761 tctgctcaag cgctgcagca acggctttca tgatgaagac aactggggtg atcttcacat
    29821 ccagcttacg tttcgccgct tcttcgttct gctgtttacg gaacgcttcc agctcggtga
    29881 tatcggtttt gtcgaagtga gtaacatgcg ggatcatcac ccagttacgg ctcaggttcg
    29941 caccagagat tttctggatg cggcccagtt ccacttcttc gatttcacca aacttgctga
    30001 agtccacctt cggccacggc agcatgcctg ggataccgcc gccagtcgct gccggagctg
    30061 cttctgcacg tttgatagct tctttcacgt aagcctgaac gtcttcgcgc aggatacgac
    30121 ctttacggcc agtgcccttc actttcgcca ggttaacgcc aaactcgcgt gccagacggc
    30181 ggatcagcgg agtcgcgtga acgtaagcgt cgttttcagc aaattcagat ttgccttccg
    30241 cttttgcagc cggagctgct gccggggctt cagcttttgc tgccggagcc ggcgctgccg
    30301 cttcctgttt cgcaggagct gcagcaggcg ctgcgccttc aacttcgaag atcataatca
    30361 gcgaaccagt tttcacttta tcgccaacgt tgactttcag ttccttcacg acacctgcaa
    30421 acggagccgg aacttccata gacgctttat cgccttctac ggtgatcagt gactgttcag
    30481 cggcaacttt gtcgcccact ttcaccatca cttcggtgac ttcaacttcg tcaccgccga
    30541 tatccggaac gttaacttct ttcacgccag ctgctggtgc aggagccgct gccggagccg
    30601 cttcctgttt agccgccgga gctgccgcgc ctgcttcacc cgcgacttcg aagaccataa
    30661 tcagcgagcc ggtagacact ttgtcaccca cgttcacttt gatctctttc acggtgccag
    30721 caaacggagc cggaacttcc atagaagcct tgtcgccttc tacggtgatc agcgactgtt
    30781 cagcttcaac tttatcgcca actttcacca ggatttcggt cacttcaact tcgtcgctgc
    30841 cgatatccgg aacgttaacg tcttttgccg ctgcagccgc tggtgctgct gccggagctg
    30901 cttctttctt ctcttctgcc tgagcaggtg cagcgtctgc tgcaccgtcg gcggaatcga
    30961 aaatcataat cagtgcgccg gtctgggttt tatcgccaac agagactttg atctctttaa
    31021 cgatacccgc ctgcggagac ggaacttcca tagaggcttt gtcgccttct acggtgatca
    31081 gcgactgttc ggcttcaact ttgtcgccca ctttgaccag gatctcggtg atttcaactt
    31141 catcagcccc gatgtccggt actttgattt cgatagccat tattctttta cctcttacgc
    31201 cagacgcggg ttaactttat ctgcatcgat gttgaatttg gcgattgcgt cagcaaccac
    31261 tttcttatcg atttcgccac gtttagccag ttcgcccagc gccgcaacca cgacataaga
    31321 agcatcaact tcgaagtggt gacgcaggtt ctcacggctg tcggaacgac cgaagccatc
    31381 agtacccagt acgcgatagt cgtcagccgg tacgtaagta cggacctgct cagcgaacag
    31441 tttcatatag tcggtagatg ccactgccgg cgcgtcgttc atcacctgag cgatatacgg
    31501 aacgcgcgga gtttccagcg gatgcagcat gttccagcgt tcacaatcct gaccatcacg
    31561 cgccagctcg gtgaaggagg tcacgctata aacgtcagaa cctacgccgt aatctttcgc
    31621 caggatctct gccgcttcac ggacgtgacg caggatagaa ccggagccca gcagctgaac
    31681 tttacctttg ctaccttcaa tggtttcgag tttgtagata cctttacgga taccttcctc
    31741 agcaccttcc ggcattgccg gcatgtgata gttttcgttc agcgtggtga tgtagtagta
    31801 aacgttctct tgtttttcac cgtacatacg ctccagaccg tcatgcatga tgacagcaac
    31861 ttcgtaagcg taagccgggt cgtaagagat acagttcggg atagtcagcg actgaatgtg
    31921 gctgtgacca tcttcgtgct gcagaccttc gccgttcagg gtggtacgac cggaagtacc
    31981 gccgatcagg aagccacgcg cttgctggtc gccagccgcc cagcacagat cgccaatacg
    32041 ctggaagccg aacatcgagt aatagatgta gaacggaatc atcggcagat tgttggtgct
    32101 gtaagaggtc gccgctgcca gccaggaaca acctgcgccc agctcgttga tcccttcctg
    32161 cagaatctgc cctttctcgt cttctttata gtaagcaacc tgctcgcggt cctgcggggt
    32221 gtactgctga ccgttcgggc tgtaaatacc aatctgacgg aacagacctt ccataccgaa
    32281 agtacgcgct tcgtcggcga tgatcggtac cagacgatct ttgatcgact tgttcttcag
    32341 catcacgttc agggcacgaa cgaaagcgat agtggtagag atctctttgc tctgctcttc
    32401 caacagcgcg ccgaagtctt gcaggctcgg cagctcaagc ttctcggtga agttcggctg
    32461 acggcttggc agataaccgt gcagtttctg acgctgagcg tgcagatagg tatgctcttc
    32521 agaaccttcc gggaaggtga tgtacggcag tttttcgata tctgcatcag acaccggcac
    32581 attgaaacgg tcgcggatat ggcgcacgcc gtccatgttc attttcttaa cctggtgcgc
    32641 gatgttttta ccttcagccg cgtcgcccat gccgtaacct ttaatggtat gagcaaggat
    32701 taccgtcgct ttacctttgg tttcctgcgc tttcttgaat gcagcgtaga ttttcttcgg
    32761 atcgtgacca ccacggttca gtgcccagat ctgctcatca gtccagtctg caaccagtgc
    32821 tgcggtttca ggatatttac cgaagaagtg ttcacgaacg tacgcaccat ctttcgattt
    32881 gaaggtctgg tagtcgccgt caacggtttc gttcatcagc tggatcagtt taccgctggt
    32941 atctttacgc agcagttcat cccaacggct accccacatc actttgatca cgttccagcc
    33001 agcaccttcg aagatgcctt ccagttcgtt gatgatcttg ccgttaccgg tgaccgggcc
    33061 gtcaagacgc tgcaggttac agttgataac gaaaaccagg ttatccagtt tttcacgggt
    33121 agcgatggtg atcgcacctt tggattccgg ttcgtccatt tcaccgtcac cgaggaacgc
    33181 gtaaacggtt tgtttagagg tatctttcag gccacggtgt tccagatatt tcaggaattt
    33241 agcctggtaa atagcaccaa tcggacccag acccatagat acggtcggga actgccagaa
    33301 ttccggcatc agtttcgggt gcggatagga agagaggcca ttgccgtgaa cttcctgacg
    33361 gaagttatcc agctgctcct gagtcagacg accttccagg aaagcacgag cgtaaacgcc
    33421 cggggagatg tggccctgga agtaaaccag gtcgccgcca tcctgctcgt tgcgtgcacg
    33481 gaagaaatgg ttaaagcaca catcataaat ggttgcggaa gactggaagg acgccatgtg
    33541 gccgcccagt tcgaggtctt ttttcgacgc acgcagaacc gtcatgatgg cgttccagcg
    33601 gatagctgaa cgaatacggc gttccagttc cagattaccc ggatactccg gttgttcttc
    33661 aacggggatg gtgttgatgt agttgctgat accggtgcct gcggctacgt tgacaccgcc
    33721 tttgcgggct tcagcaagca gttggtcgat cagatactga gcacgctcaa caccttcttc
    33781 acggatgacc gattcgatcg cctggagcca gtcgcgagtt tcgatcggat ccacgtcatt
    33841 tgggaaacgt tctgacatgg gttattcctt atctatctaa taacgttgag ttttctggaa
    33901 cctgtcccat tgaactctcg ccggaaagcc caataagaca ggttctacgt ttagttgccg
    33961 cgcttttata tgcgcttgat ttacaacatc ttctggataa tttttaccag aaaaatcact
    34021 aattctttcg ttgctccaga cgacgcagag aacgctcacg gcggctctct tcacggcttc
    34081 tgtcgagcaa aatttcttcg ataaaggcca gatggcgatg cgatgcttcg cgcgcttctt
    34141 ccggcttacc ggccataatc gcttcaaata tgcgggtgcg gtgactactc accagcggca
    34201 gcatctcgcg acgcgaatag agcaattcga agttctggcg gacattctgg gccaacatcg
    34261 gctccataca ccttagcaga tgaagcagaa ccacattgtg ggccgcttcg gtgacggcaa
    34321 tctgatactg gagtacggcg tttgattccg cgtccagatc gccagactgc tgcgccagtt
    34381 ctatggcgtg gtggagttca cggatgcgtt ccttgtcttc atcggtacta cgcagcgcgg
    34441 cgtaataagc ggcgatacct tccagggcgt gtcgtgtttc gagcaagtca tactgtgatt
    34501 caggatggtc ggagagcagc tctaccagcg gatcgctgaa gctttgccat aggctgctct
    34561 ggacaaaagt gccgccaccc tggcgacgaa gcaacaagcc cttcgcttcg agacgttgaa
    34621 tcgcctcacg caatgaggga cgggagacgt caaactgttt tgccagttcg cgttccggtg
    34681 ggagtttttc gcccgggcgg agagtgcctt cgaggatcaa aaactccagt tgctgctcaa
    34741 tcacatcgga gagttttggt tggcggattt tgctgtaggc catgagttcc tgtcttaagc
    34801 cacttgccga agtcaattgg tcttaccaat ttcatgtctg tgacgctaaa gtaacaaagt
    34861 attcacctta tgtccataca ggttttgatt gaaatcatga aactgtgcac attttaacaa
    34921 cttgacatat ataacgtttc aaagttgtaa ctatgcacaa atgtagactt tacg
//
LOCUS       DAPGOF010000030        34743 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.030, whole genome shotgun sequence.
ACCESSION   DAPGOF010000030 DAPGOF010000000
VERSION     DAPGOF010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.030"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..776
                     /locus_tag="RHV13_001282"
     CDS             <1..776
                     /locus_tag="RHV13_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HDV1240836.1"
                     /translation="YSFPAYLPRFSSTDKPAKLKFHQYARATLLSNRGRDHNCDGRRT
                     VEIHKLDLSDWQAFNKLAKKCNAYDGVTMNGDNSFGWNHEATLDNIHAQKYNKAYAGA
                     RLTAELKYLLQDVESFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVTLPAQMPNCWS
                     SELPTIDLYIDQLRTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSSSAAIYSGL
                     GFERVPQERDISCARMYLTPANHPDLWTQENGEWIYLRN"
     gene            complement(847..1296)
                     /gene="rplI"
                     /locus_tag="RHV13_001283"
     CDS             complement(847..1296)
                     /gene="rplI"
                     /locus_tag="RHV13_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDV1240837.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(1338..1565)
                     /gene="rpsR"
                     /locus_tag="RHV13_001284"
     CDS             complement(1338..1565)
                     /gene="rpsR"
                     /locus_tag="RHV13_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDV1240838.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(1570..1884)
                     /gene="priB"
                     /locus_tag="RHV13_001285"
     CDS             complement(1570..1884)
                     /gene="priB"
                     /locus_tag="RHV13_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HDV1240839.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(1891..2286)
                     /gene="rpsF"
                     /locus_tag="RHV13_001286"
     CDS             complement(1891..2286)
                     /gene="rpsF"
                     /locus_tag="RHV13_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDV1240840.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            2613..2888
                     /locus_tag="RHV13_001287"
     CDS             2613..2888
                     /locus_tag="RHV13_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDV1240841.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(3017..3703)
                     /gene="ulaF"
                     /locus_tag="RHV13_001288"
     CDS             complement(3017..3703)
                     /gene="ulaF"
                     /locus_tag="RHV13_001288"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HDV1240842.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(3703..4557)
                     /gene="ulaE"
                     /locus_tag="RHV13_001289"
     CDS             complement(3703..4557)
                     /gene="ulaE"
                     /locus_tag="RHV13_001289"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HDV1240843.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            complement(4567..5217)
                     /gene="ulaD"
                     /locus_tag="RHV13_001290"
     CDS             complement(4567..5217)
                     /gene="ulaD"
                     /locus_tag="RHV13_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDV1240844.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADISAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAAREFKRSIAELWG"
     gene            complement(5231..5695)
                     /gene="ulaC"
                     /locus_tag="RHV13_001291"
     CDS             complement(5231..5695)
                     /gene="ulaC"
                     /locus_tag="RHV13_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HDV1240845.1"
                     /translation="MKLRDSLAVNKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDGVEQFGPYFVIAPGLAMPHGRPEEGVKKTGFSLVTLKKPLEFNHDDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(5705..6010)
                     /gene="ulaB"
                     /locus_tag="RHV13_001292"
     CDS             complement(5705..6010)
                     /gene="ulaB"
                     /locus_tag="RHV13_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HDV1240846.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            complement(6026..7423)
                     /gene="ulaA"
                     /locus_tag="RHV13_001293"
     CDS             complement(6026..7423)
                     /gene="ulaA"
                     /locus_tag="RHV13_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDV1240847.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIACMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            7778..8842
                     /gene="ulaG"
                     /locus_tag="RHV13_001294"
     CDS             7778..8842
                     /gene="ulaG"
                     /locus_tag="RHV13_001294"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HDV1240848.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            8950..9705
                     /gene="ulaR"
                     /locus_tag="RHV13_001295"
     CDS             8950..9705
                     /gene="ulaR"
                     /locus_tag="RHV13_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HDV1240849.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(9702..10451)
                     /gene="yjfP"
                     /locus_tag="RHV13_001296"
     CDS             complement(9702..10451)
                     /gene="yjfP"
                     /locus_tag="RHV13_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDV1240850.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTLKCTASMMGSGYFTSLARSLFPPLIPETTA
                     QQNEFNNIVAPLAEWEATNHLEQLSDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KRLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            10633..10962
                     /gene="bsmA"
                     /locus_tag="RHV13_001297"
     CDS             10633..10962
                     /gene="bsmA"
                     /locus_tag="RHV13_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HDV1240851.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            11111..11386
                     /gene="yjfN"
                     /locus_tag="RHV13_001298"
     CDS             11111..11386
                     /gene="yjfN"
                     /locus_tag="RHV13_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HDV1240852.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(11503..13128)
                     /locus_tag="RHV13_001299"
     CDS             complement(11503..13128)
                     /locus_tag="RHV13_001299"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HDV1240853.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQCNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(13212..14375)
                     /locus_tag="RHV13_001300"
     CDS             complement(13212..14375)
                     /locus_tag="RHV13_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HDV1240854.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(14378..15016)
                     /locus_tag="RHV13_001301"
     CDS             complement(14378..15016)
                     /locus_tag="RHV13_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HDV1240855.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(15026..15424)
                     /locus_tag="RHV13_001302"
     CDS             complement(15026..15424)
                     /locus_tag="RHV13_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HDV1240856.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(15442..16101)
                     /locus_tag="RHV13_001303"
     CDS             complement(15442..16101)
                     /locus_tag="RHV13_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HDV1240857.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKVMGAMILNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGIDIPLTSLHII
                     G"
     gene            complement(16152..16850)
                     /locus_tag="RHV13_001304"
     CDS             complement(16152..16850)
                     /locus_tag="RHV13_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HDV1240858.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(16869..17270)
                     /locus_tag="RHV13_001305"
     CDS             complement(16869..17270)
                     /locus_tag="RHV13_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HDV1240859.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNSESALIIKMNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALS
                     LKSSLEDILLEITSLVDNALDLAEITEEYSH"
     gene            complement(17397..18128)
                     /gene="rlmB"
                     /locus_tag="RHV13_001306"
     CDS             complement(17397..18128)
                     /gene="rlmB"
                     /locus_tag="RHV13_001306"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDV1240860.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(18309..20750)
                     /gene="rnr"
                     /locus_tag="RHV13_001307"
     CDS             complement(18309..20750)
                     /gene="rnr"
                     /locus_tag="RHV13_001307"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDV1240861.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(20789..21214)
                     /gene="nsrR"
                     /locus_tag="RHV13_001308"
     CDS             complement(20789..21214)
                     /gene="nsrR"
                     /locus_tag="RHV13_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HDV1240862.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKSASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(21419..22717)
                     /gene="purA"
                     /locus_tag="RHV13_001309"
     CDS             complement(21419..22717)
                     /gene="purA"
                     /locus_tag="RHV13_001309"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDV1240863.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(22821..23018)
                     /locus_tag="RHV13_001310"
     CDS             complement(22821..23018)
                     /locus_tag="RHV13_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HDV1240864.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(23100..24104)
                     /gene="hflC"
                     /locus_tag="RHV13_001311"
     CDS             complement(23100..24104)
                     /gene="hflC"
                     /locus_tag="RHV13_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HDV1240865.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(24107..25366)
                     /gene="hflK"
                     /locus_tag="RHV13_001312"
     CDS             complement(24107..25366)
                     /gene="hflK"
                     /locus_tag="RHV13_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HDV1240866.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(25452..26732)
                     /gene="hflX"
                     /locus_tag="RHV13_001313"
     CDS             complement(25452..26732)
                     /gene="hflX"
                     /locus_tag="RHV13_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDV1240867.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(26809..27117)
                     /gene="hfq"
                     /locus_tag="RHV13_001314"
     CDS             complement(26809..27117)
                     /gene="hfq"
                     /locus_tag="RHV13_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HDV1240868.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(27203..28153)
                     /gene="miaA"
                     /locus_tag="RHV13_001315"
     CDS             complement(27203..28153)
                     /gene="miaA"
                     /locus_tag="RHV13_001315"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDV1240869.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEIDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(28146..29993)
                     /gene="mutL"
                     /locus_tag="RHV13_001316"
     CDS             complement(28146..29993)
                     /gene="mutL"
                     /locus_tag="RHV13_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDV1240870.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVVAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(30003..31340)
                     /gene="amiB"
                     /locus_tag="RHV13_001317"
     CDS             complement(30003..31340)
                     /gene="amiB"
                     /locus_tag="RHV13_001317"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HDV1240871.1"
                     /translation="MMYRIRNWLVATLLLLCAQVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVAKRVETPTVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(31359..31820)
                     /gene="tsaE"
                     /locus_tag="RHV13_001318"
     CDS             complement(31359..31820)
                     /gene="tsaE"
                     /locus_tag="RHV13_001318"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDV1240872.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(31792..33339)
                     /gene="nnr"
                     /locus_tag="RHV13_001319"
     CDS             complement(31792..33339)
                     /gene="nnr"
                     /locus_tag="RHV13_001319"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HDV1240873.1"
                     /translation="MTDHTMKKNPVSIPHTVWHADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVALIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            33338..34477
                     /gene="queG"
                     /locus_tag="RHV13_001320"
     CDS             33338..34477
                     /gene="queG"
                     /locus_tag="RHV13_001320"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDV1240874.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAMAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(34460..34513)
                     /locus_tag="RHV13_001321"
     CDS             complement(34460..34513)
                     /locus_tag="RHV13_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1240875.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         8380 a         9601 c         8510 g         8252 t
ORIGIN      
        1 tttattcatt ccccgcatat ttacctcgtt tttcgtccac cgacaaacca gcaaaactaa
       61 aatttcatca atacgcacgc gctacattac tgtcaaatcg tggaagagat cataattgcg
      121 atggcagacg tactgttgag atccacaaac tagatctctc agactggcaa gcattcaata
      181 agttagcaaa gaagtgtaat gcttacgatg gagtaacgat gaatggtgat aattcttttg
      241 gatggaacca tgaagctaca ttagataata ttcacgccca gaaatacaat aaagcttacg
      301 cgggtgcgag gttaaccgca gaactgaaat atttattgca agatgtcgaa tccttcgaac
      361 ccaacagtaa atacacaata cacgaagtgg ttttgggacc tggttatggt actccagact
      421 ataccggcca aacgatagga tacgttgtaa cccttcctgc ccaaatgcct aattgctggt
      481 catctgaact accaacaatc gatctttata tcgaccaact tcgcactgtt acaggcgttt
      541 ctaatgccct cggattcatc attgctgcat tgctaaacgc gtattctgat ttaccccatg
      601 atttgaaaat tggtctgcgt tccttatcaa gctcagccgc aatctactcg ggtttgggtt
      661 ttgagcgagt gccgcaggag cgagacatca gttgcgctcg tatgtatcta acgccagcaa
      721 accatcctga cttatggaca caagagaatg gtgaatggat ctacttgcga aactaatacc
      781 tgacgaaaaa tagaaagcaa aacgccgacc aatggtcggc gtttttacgt ctcgttgaat
      841 aacgaattat tcagctacta cgtttacgat cactttcgcg aatacttcgc tgtgaacctg
      901 gaagctcact tcgtgttcgc cagtggtacg cagaacgccg ttcggcagac gaacttcgct
      961 cttagccact tcaacgccag ctgcagttac agcgtcagcg atgtcgcgag taccgatgga
     1021 accgaacagt ttaccttcgt cgccagcttt agacgcgatg gtaacagttt ccagtgcatt
     1081 gattttctca gcgcgagcat tagcagctgc cagaacttca gccagtttag cttccagttc
     1141 agcgcgacgt gcttcgaaga attcaatgtt tttcttggta gctggaacag ctttaccctg
     1201 cggtaccagg aagttacgag catagcccgc tttaacgttt acctgatcac ccaggctacc
     1261 caggtttgct actttatcaa gcagaataac ttgcattacc ttatcctctc aaagtcgtat
     1321 taatggaccg tgaccgatta ctgatggcga tcagtgtacg gcagcaggga caggtagcga
     1381 gcgcgtttga tagcgcgagc cagctgacgc tggtattttg cacgggtacc ggtgatacgg
     1441 cttgggacaa tcttaccgct ttcggtgatg tagtttttca gcgtagcgat atctttatag
     1501 tcgatctctt gaacgccttc cgcggtgaaa cggcagaact tgcgacgacg gaaataacgt
     1561 gccatatggc tagtctccag aatctatcaa ttcaatctgc tcggcatgca aaaccatttt
     1621 gctcagtccg ttctttgcct tgtggcatga aatgaacccc tgaacggtta tgcgactgcc
     1681 gaccgttata ctgtgagtaa tggcctggtt ttcgtgtccg ctaacaataa cgggcatttg
     1741 acaccatgcc tgccggtgaa agccggcttc ctcctgcaca gaacgatgct caagcacgaa
     1801 ctggcagtga ggaattcctg atggactgac ctttcgaagg ggagtcctgc acacggtgcc
     1861 ggacaacacc agacggttgg tcatcagaaa ttactcttca gaatccccag cttcagcatc
     1921 atcagcggtt tcgtttgcga aatcatcgcg acgctcacgg cgctcgtctt tcgctttaac
     1981 catcggagat gcttcggtaa cagcgtgctt ggtacgcata accatgctgc ggataacggc
     2041 atcgttgaag cggaaggtag tttccagctc atcgatcact tcctgcggag cttcaacgtt
     2101 catcagaacg tagtgagctt tgtgcagttt gttgatcggg taagccagct gacggcggcc
     2161 ccagtcttcc agacggtgga tcttgccttc tgcaccagtg atggcagcag tgtagcgctc
     2221 gatcatgccc ggaacctgtt cgctctggtc aggatggacc ataaaaacga tttcgtaatg
     2281 acgcatcgaa ttgctcctta cggattattc agcctcctgt ctgggtcagc cgaatcccgg
     2341 ggaggcaagg aacgtgttaa aggtcggctg aaaaatgacg cgttatagta ctgacatacc
     2401 ccgctaaact caaggccagc cgacaaataa atctcatctt tcaccaaagt ggactaacac
     2461 gctgatttga agttcatcac acttcatcat ggtgtttttt tgaacaactg catcagaaat
     2521 ggtcaccatc aacttcctta tcattgatta aaattaaatc agaagatcat cacagaggag
     2581 agcacacaaa gtcagccgtc gaggaggtat ctatgttcag tcgtgtttta gcccttctgg
     2641 ctgtgctttt actaagtgca aatacatggg cagccattga aattaataac caccaggcca
     2701 gaaatatgga cgatgtgcaa agcttaggcg tgatttatat caatcataat ttcgccactg
     2761 aaagcgaagc acgtcaggca ttaaatgaag aaacagatgc gcaaggcgca acgtactacc
     2821 acgtaattct gatgcgggaa ccggggagta acggcaatat gcacgccagc gcggatattt
     2881 atcgctagca ccaggtataa ccaacgaaac attgccatag tttgctttgc ccccttcgca
     2941 gggggctttt tttgaaaaac ggctgcggaa tcagaccagt tatctcccga ataaggaaat
     3001 ttccgcagcg cgtgttctac ttctgcccgt aataagcgtt aggaccgtgt ttacgcatga
     3061 agtgtttatt catcaggaag ctgtcgatgt gattgagttg tgggttaata ctgcgggcaa
     3121 tccacgccat tttcgccact tcttccatca caaccgcgtt atgcaccgca tcgtgggcat
     3181 ctttccccca ggcgaacggt ccgtgctgat acaccacaat tcccggcgta tgcagcggct
     3241 cggcgttgcc cagcgtttcg ataatcactt tgccagtgtt cagttcatac tcaccctgca
     3301 cctcttcttc gcttaatccg cgcgtgcacg ggatgtcacc aaagaagtag tcggcatgcg
     3361 tagtgcctaa cgccgggatc gccagccccg cctgcgccca tgcggtggca tgagtggagt
     3421 gggtatggac aatgccgcca agcgacgggt aacgacggta gagttcgaga tgcgtcgcgg
     3481 tgtcggaaga cggacgatat tccccttcca ccaccttacc gctcatatcg accaccacca
     3541 tatcgtctgc tttcatggtt tcgtaggcga cgccgctggg cttgatcacc accagcccgc
     3601 gttcgcggtc gatagcgctg acgttgcccc aggtaaaggt caccagccca tagcgcggca
     3661 gatccatgtt ggcttcaaac acctgctgtt ttagcttttg cattatgccg cctccaccat
     3721 gcccgctttc gccatgcgcg ctttcaccca atcacgcgct ttcgccactt ctgccgccgg
     3781 gtcttccgcc gtttcgctcc acatctcaat caggtacggc ccgcaatagc cactctgttt
     3841 gagcgtttcg aaacaacgtt cgaaatccac tacaccttcg ccaaacggca cgtttttgaa
     3901 gacgccaggt ttggtgtctt tcacatgtac cgcgacgata tgcccgattc cggcctgcaa
     3961 ctccatctgc acatcgttgt cccacgccga caggttgccg atatccgggt agagctggaa
     4021 ccacggattg ttgagatagt gcgcgtagcc cagcgccttg ctgatggagt tcatcaacgg
     4081 ataatccatg atctccatcg ccagcgtcac ctgcgcgcgg cttgccatct caacgctctc
     4141 tttcaggccg tcacggaaac gacgacgcgt ttcgttattg gcttcctgat agtaaacgtc
     4201 atagcccgcc agctggatca cgcgaatacc gacatcctgg gcgaactgga tagctttacg
     4261 cataatctcc agcccctgcg cccgcaccgc gtcatcttca ctgcccagcg ggaaacgacg
     4321 atgagcagaa aggcacatgg acggcacgcg cacgccggtt tcaacaatcg cattgaccag
     4381 cgccagacgc tgctcgcggc tccagtcgag gcgcgacagg cgctcgtccg tttcatctac
     4441 cgacatctcg acaaaatcga agcccaacgt ttttgccagt tgcaggcgtt ccagccagca
     4501 ctccccagcg gggagcgctt tttcatagat gccaagcggg atttgtttgg acaacatatc
     4561 cgctccttag ccccacagtt cagcgatgga acgtttgaac tcacgtgcgg cttccaccgg
     4621 agaagcggca tcacggatac tacggcccgc gataaagacg tgaatcggaa tacccttgaa
     4681 cagcggcaga tcttccagcg ccaggcctcc ggtgacggtg actttgaagc ccatatcgga
     4741 aagacgtttg atcgcgctga tgtccgcttc gccccacgcc acgcctgcgg cctgcgcgtc
     4801 acggctgcgg tgataaacca cctgctgaat gcctgcatcg cgccactgtt gcgcctgttc
     4861 ccaggtccag taaccggtca gttcgatctg cacgtcgccg ttaaactctt ttgccacgtc
     4921 cagcgcgcct ttggcggtgt tgatatccgc acagcaaatt accgtcaccc agtcagcgtt
     4981 ggcttcgaag cacatacgcg aaaggatttt gcctgcatcg gcaattttgg cgtctgccag
     5041 tacgattttg tgcgggtaga gcgctttcag gtcacgaacc gcacgcacgc cttcgcccac
     5101 gcacagaatg gtgcccactt cgataatgtc gacttcttcg gcaatcaggc gagtggtttc
     5161 gtaggcgcta tccatagtct ggttgtccag cgcgacttgc aacatcggta atgacatgtt
     5221 caattccttc ttaagctgcc gcgttggtgc ggtcaatgag atccagtact tcctgctcgg
     5281 tacggcaggc gcgtaaacgg tcaaaattct cttcatcttc aaacaggttg acgatctgca
     5341 tgatgcccac ttcctggtga gtattggcat cgaccgccgc catggtgatg aggatatcca
     5401 ccggatcgtt gtcgtcgtgg ttaaattcca gcggcttttt cagcgttacc agagagaaac
     5461 ctgttttctt aacaccctct tccggccgcc cgtgcggcat cgccagcccc ggagcgataa
     5521 cgaaataagg gccaaactgt tccacgccat ccagaatcgc ctggtaatag cgcggctcga
     5581 caacgtccgc cgccaccagc agatcgacgc cgattttcac cgcgtcctgc caggtttcag
     5641 cttcagcctg caggcggatg gatttattta cagccagcga atcacgtaat ttcatggcgc
     5701 gtccttactt cacgtcctgc gggaaatgct ctttgatcac ttccagcagt ttcgggccaa
     5761 agtcggcagg agagagcatg ttgcgcacgc caaccacgta tttgttgccg gtcacggtga
     5821 tttcgcccgc aatgtgcgta gaagcgatga tgatatccgc gccactcaac tcgcttttgt
     5881 actcgccaac cgcgcagctg tttaccgtat ggtcaatgtt tgattgggtt aaaaactggt
     5941 ccactttcat cttcatgatc atggaactgc cttgtccgtt gccacacaca gccagaatac
     6001 gtacggtcat aatcaaaact ccttattaag cagactgttc tgccagttgt ttttctgcat
     6061 cttcttctgc gcgcagtgcg cggccagcga agaacatata agccagtgca attacaatga
     6121 tgacggccat acaggcgata ccgatggaga agaagccttg catcatcggc ggtgccagaa
     6181 tcgaccagtc cgccatgccc atccaggcac tcataccggt gagtttcacc gcccagacgc
     6241 aaccaaagat ttcgatcatt cccatcacca gacaaatctt cagcgctgca cgccagccgc
     6301 cgaagtggtt agcgaacacg ccgatggtgg cgttggagaa gaacatcggg ataaagcctg
     6361 gaataatcag aatggaagag ccgcaggcga ccaggatgcc aactgcaatc agctgaccga
     6421 tggtgcccca cataaagccc cagaccacgg cgttcggcgc gaagctatag atagccgcac
     6481 agtcaatcgc cagaaccgca cctgggatca ggcgctggga gataccgtta aatgcttcag
     6541 agagttccgc cacaaacatg cgcacgccct gagtgatgat gaagatcgcc accgcgaagg
     6601 agaaaccggt ttgcaggatg tagaccgtcc agtgcacttt gcccgccatc gcctgcacgg
     6661 tgtcgatacc gaaggagagc agaatggcgc caaagaagat ggtcatcaca atcgccgtgg
     6721 agacgatgtt gtcgtggaaa atgttcagcc agcccggcaa tttgaggtct tcaacgctct
     6781 cctctttttt gccgaggaac ggcgcaactt tataggcaat ccatgatgca aactgctgct
     6841 ggtgaccgat ggagaaaccg cagccgtccg tcacttcctg agtcggcttg tacatcatgt
     6901 tggaggtaat gccccagtag agcgaaacca gaatcgccgt gcagataatg gtcgtccaca
     6961 tggaatagcc gaaaataaac aacgtaacgg caatcagccc cgcctgctgg aacatgatat
     7021 gaccagtcag catgatggta cgaataccgg taatgcgacg cagcagcacg taacagatgt
     7081 tcagtgccag cgctaacaac accgcgtaac ccacccagct ataggcatcg cccatgcggt
     7141 cgatggttgc catcatcgac gcgtaagtat cggaaattgc gccgttaatg ccgtagactt
     7201 cagacatttt cgccaccacc ggtttgaagg tgctggtgag gatgccggac cctgcctgca
     7261 acaacatgaa accaattatg gttttaatcg tgcctttaat aataacgctg acacttttgc
     7321 gcagtaggat gtagcccaga caggtcacaa tacccagcaa caacggggca ttggtcatga
     7381 cctggttaaa aaacacggta aagatgttgt agaggatctc cataacgatc tcctgaagaa
     7441 gaggtaaccg ggtattcctc acacccggaa tgttgtgcac tcactctaat tttcaaaagt
     7501 aatcacaaca agattattta tgattaaatg tgacgcgaca cacaaataat tccataacga
     7561 aaatatggga ttcacacaac gaaacaatta atcatttaaa ttcattaagt taattgaatt
     7621 acacattcca ttaaatcttt ccacactcca aattcacaac aattcacccg gcaaaaagtc
     7681 aaaaaatggt cattgccttc ctgaaaaaat agtgccagga ttaatcaaaa ccaatcacat
     7741 attgattcga attgaactat catgaaggta aatggcgatg agtaaagtga aaagtatcac
     7801 ccgagaatcc tggatcctga gcactttccc ggagtggggt agctggctga atgaagaaat
     7861 tgaacaagag caggtcgccc ctggcacatt tgcgatgtgg tggctaggct gcaccgggat
     7921 ctggttgaaa tcagaaggtg gcaccaacgt ttgcgttgat ttctggtgcg gcactggcaa
     7981 acaaagtcac ggtaacccgt taatgaaaca gggtcaccag atgcagcgca tggctggcgt
     8041 gaaaaaactg cagccaaacc tacgcaccac cccgtttgtt cttgatccgt ttgcgattcg
     8101 ccagatcgac gcggtactgg cgactcacga tcacaacgat catatcgacg ttaacgtcgc
     8161 tgctgccgtg atgcagaact gtgctgatga cgtaccattc attggaccga aaacctgcgt
     8221 tgatttgtgg attggctggg gcgtaccgaa agagcgttgc atcgtggtca aaccgggcga
     8281 tgttgtaaaa gtaaaagaca ttgaaattca tgcgcttgac gctttcgacc gtactgcact
     8341 gatcaccctg cctgccgatc aaaaagcggc tggcgtactg ccggacggca tggacgatcg
     8401 cgcggtgaac tatctgttca aaacgcctgg cggctccctg tatcacagcg gcgactccca
     8461 ctactctaac tattatgcga agcacggtaa cgaacatcag atcgatgtgg cgttaggatc
     8521 gtatggtgag aacccgcgcg gtattaccga caaaatgacc agcgccgata tgctgcgtat
     8581 gggtgaagcc ctgaatgcga aagtggttat cccgttccac cacgacatct ggtcaaactt
     8641 ccaggccgat ccgcaagaga tccgcgtgct gtgggagatg aaaaaagatc gtctgaagta
     8701 tggcttcaag ccgtttatct ggcaggttgg cggcaaattt acctggccgc tggataaaga
     8761 caacttcgag taccactatc cgcgcggttt cgatgattgc ttcactattg aaccggatct
     8821 gccgttcaag tcattcctgt aatctgtcgt ttataccgga tagcgtggag aagttcgcgc
     8881 tatccggcgt agtatttcct ggctatttca aatatcatct aaaaaaatca aattttatcg
     8941 gaatagctca tgactgaagc acaaagacat caaatcctcc tggaaatgct cgcacaattg
     9001 ggctttgtga ccgttgagaa agtcgttgag cgtctgggaa tttcgcctgc cactgcgcga
     9061 cgcgatatca ataaacttga cgaaagcggc aaactgaaaa aagtgcgcaa tggcgcagaa
     9121 gctattaccc aacagcgccc gcgctggacg ccgatgaatc tgcatcaggc gcagaatcac
     9181 gatgaaaaag tacgtatcgc taaagcggcc tcgcagctgg ttaatccggg cgaaagcgta
     9241 gtcatcaact gcggctccac cgcgtttctg cttgggcggg aaatgtgtgg caagccagtg
     9301 caaatcatca ctaattatct accgctggca aattacctga tcgatcaaga acatgacagc
     9361 gtgatcatta tgggcggaca gtacaacaaa agtcagtcca tcactttaag cccgcagggc
     9421 agcgaaaaca gtctctatgc cgggcactgg atgtttacca gcggaaaagg gctgaccgca
     9481 gaagggttgt ataaaaccga tatgctgaca gcaatggcag agcagaagat gctgagcgtg
     9541 gtagggaaac tggtggtact ggttgatagc agtaagattg gcgaacgcgc gggaatgctt
     9601 tttagccgtg ccgatcaaat cgatatgctt atcaccggca aaaatgctaa cccggaaatc
     9661 ctgcaacaac tggaagcgca aggggtcagc attctgcgtg tttaaagatg ctggcggaaa
     9721 aatgtcacgg cagcatctaa cgcctcaggc gtaatgcggt gacgcacgcc tggctgccat
     9781 gaacaggtta gccgtttatc ccgtcccgtc tcgcttaagg cctgctgcaa acgtagtgat
     9841 tcgtcggcag gcacaacgtc atcgtcgagg ccatgccaca gcagtagagg tctgtcacta
     9901 agttgttcca ggtggtttgt cgcttcccac tctgccagcg gcgcgacaat gttattgaat
     9961 tcattctgct gtgctgtcgt ttcaggtatc agcggtggaa acagtgaacg ggcgagcgat
    10021 gtaaaatagc ccgatcccat catgctggcg gtacatttca acgtggggtg gcgagcggta
    10081 attcccagtg ccgtcatcgc gcccatcgac gcgccaccga ctgcaagacg gtcatcaagc
    10141 agccagtttt cttcggctat tgccgcacgt aaagtagtga attcctgcat actttgtagc
    10201 aagatttgcc agaattgatt taaccgccgc gctgcgtcgc cactaaaacg actaccgtga
    10261 tcgggcgcat ccggcatgat cacccgcaaa ccagcctgcg ccagcgcaac ggcaaaatag
    10321 ctatacacca gactggacga agtaaagccg tgataaaaaa tcacgcacgg taacggggta
    10381 tctttttgtc cgacaggata agcatgaaga acgggaatat ctgccagctc gcgtgattct
    10441 atttcaatca tctgtctctc ctttttgtct ggtatgacat gaagaaatcc ttagcacaag
    10501 tttctgctgg cggtattagt tcataatgtt gagatgtggg ttacgctttc gtcacgtttt
    10561 cattcgaaaa tcgcgtacca ggtaacaatt cgggaacatt cccccaaagt taaattaata
    10621 aggcactaca ctatggttag caggaaacgt aatagcgtta tttaccggtt tgccagttta
    10681 ttattggtgt tgatgttaag tgcctgtagc gcactgcaag gtacgccaca gccagcaccg
    10741 ccagtaacgg accatccgca agagattcgc cgcgaccaga cgcaagggtt acagcgaata
    10801 ggtagcgtaa gcacgatggt tcggggttct ccggatgacg cattagcaga aatcagagca
    10861 aaagctgtcg ctgcaaaagc tgattattac gttgtcgtca tggtggacga aaccatcgtg
    10921 acaggacagt ggtattcaca agccattttg taccgtaaat aaatcagaca acttacatag
    10981 tcagctttac attgctttgc gtccctatgc gtttacctgt gcacaataaa ttaccggctg
    11041 gaagcctggg cgtttattac cgcgatggaa tgccctgcaa catgtgggga aacgaaaaat
    11101 ggagctgacg atgaaacaat tacttgcctc accctcgctg caattagtga cttatcctgc
    11161 gagcgccacg gcacagtctg cagaattcgc cagtgctgac tgcgtgacgg gcctgaatga
    11221 aataggtcag atctcagtta gcaatatttc aggggatccg caggacgtgg aacgtatcgt
    11281 ggcgttaaag gccgatgaac aaggtgcgtc atggtaccgc attattacca tgtacgaaga
    11341 ccagcagcct gataactggc gcgtacaggc gatactgtat gcatgagtgt catctacagc
    11401 aaatgaacaa tgccggatgc ggcgcgaacg ccttgtccgg cctacaattt tcacatattt
    11461 catttagtta atcgaaacca gcgtcgcatc agtcgtatac gcttacacac acactccccc
    11521 cgtcgcccgc agcaataaat cattctggat ctgctctgac aaccgtacgc cgccgcgcgt
    11581 atccaacatc acctgacacc acgcctgcgc cattggcgga gaagcatatt tcaacatttg
    11641 cgcaccgcag cccagcagga atagctgatg agtaatctct cgccccagtt cttcagctgg
    11701 cttacgcagc tgctgctgta aacgacgaac cgcgcgatca aaatagcgat cctgcccttt
    11761 cacttccaca aatgcttccg acaataagtc gtatacgccc gcttgcttat tgagaacgcg
    11821 caacacatcc aggcacataa tattgccgga accttcccaa atactgttta ccggcatctc
    11881 ccggtaaagc cgcggcagct cactctcctc gcaataacca atgccgccca gcacctccat
    11941 cgcttcggcc acaaacggca tacctcgttt gcagatcaca aattttgccg caggcgtaaa
    12001 taaacgcgcc cacagggctt ctttggcatc ggcacgccgg tcccacgctc gcgcaagacg
    12061 aaacagcaac gccgtttgcc cttcaagctg aagtgccatg cgacttaaga catgacgcat
    12121 aaggggctgt tggatcaatg gattaccaaa aacatggcgt tgatgtgcat gataaatcgc
    12181 cagcgaaaat gcacggcgca tcatggcatg gctacccagg gcgcaatcaa aacgcgtcat
    12241 cccccccatt ttcaggatca gacgaattcc ttccccttcc tgccccaata accagccaat
    12301 ggcatcctga aactccactt cacaactggc gttagagcga ttacccagct tatctttcag
    12361 ccgctcgagg cgaatcgcgt tgcattgccc gtcaggcaaa aagcgcggca caaaaaagca
    12421 ggacagaccg cccgcggtct gcgccagcac cagatgcgca tcgctttgcg gcaccgagaa
    12481 aaaccattta tgccccacca gccgataaga gccatcctcc agacgctctg cgcgggtggt
    12541 gttgctcatg acatcggaac cgccctgctt ttccgtcatt cccatgccaa tcaacaaacc
    12601 gcgtttttgc ccacctggca ataagtgaga atcgtagcga tcgctcaaca gcggcgtggt
    12661 ccagtcctga aacggcgcgg gtaacatctg taacaacaat ggcgtggcgg caaaggtcat
    12721 cgttattgga cataacgacc cagcctcaac ctgcgcatgt aacataaaac gcgccgcgcg
    12781 cgccacaaat gcgccggagc gagcgtcttc ttcccaggca agattgtgca cccgattggt
    12841 acatagtgcc tgcatcagca ggtgccaggc ggggtgaaaa cgcacatcgt ccagacggcg
    12901 tccttgcgca tcgtagcgca ataattcagg cggattcaca ttcgccagcc gccccagttc
    12961 aagggattca gccgttccta actgctgacc aatactggcg agaaaatcgc tatcccagcc
    13021 agcaccttca cgcgttaccg cttcgcagag cgcgccatca gacaggtata aattactgtt
    13081 atttaagggt ataggttgat taaaaacagt gtgagtttgc cagtgcactg tgtctccctc
    13141 catcaatggc agtcaccatt agtatggtca ctgccatgga ttcctgacag aaagagattg
    13201 ctaaaacatt cttatccagc aatgtaatgc ggaacgaaac gtgaggtgtc tttggtgatc
    13261 agcgtgttat cttcacggat ccccattccg cacgcttcat catcgacaat ccagctaccg
    13321 atgagtgtgt agctatcgcc aaatcgcggc agaggttgaa acgcctggta gatcatcggt
    13381 tcatcggcgt aatcaccatc agcgtggtca acgacgttat tctgaccgtc gaaaatggtg
    13441 acgtttccgc cttcgcgcga gtagatcggt ttacgcacat agctttcgcc agcggcaatc
    13501 tgcggttttt cgccctcaaa ccaggatgca agaaggttag gatgaccagg gaagaagcgc
    13561 caaagcagcg gcattagccc tttattactc aagatacttt tccataacgg ctccacccat
    13621 tgctcgcgac gcttgcgcag cagcggaccg ttatcgtcac gcatcatcca ctccagcgga
    13681 tacagcttaa atgcacgctg gatgacatta tcatcaagat cggtcagtac accgccgacg
    13741 cccaaaccga gatcttcaat gtagataaac cgcgactcct gccctgcctg ctgggcgcag
    13801 tcctgcaagt acagcacggt agtcctgtct tcatcggtgt cctgacagca gcagaaataa
    13861 aacggctccc ggctataaag ctcgctaaag cgtgaaatca ggcgttcctg aatggcattg
    13921 tactgatcgg catcacgcgg aataatgccg ctgcgccggg catcctccag ccacaaccac
    13981 tggaaataag ccgactcata caacgaggtc ggcgtatcgg cgttgtactc cagcagcttc
    14041 accggcgcat tgccacacca ggcaaaatcc atccggccat acagcgaagg atcgcgtgcg
    14101 cgccagcttt cggcgatcac atcccagtac aacggcggga tcgccagttg cgtcaggatc
    14161 tcttcgtctt taaccgcgcg atcaaccacc tcaaggcaca tctgatgcag ttccgcagtc
    14221 ggtttttcga tctgctcttc aatctggcgc agagtaaaac ggtaagcccg actctcgtcc
    14281 caatagattt cattgtcgat gatatgaaag tcgaaaccgt tgttagcggc gatctggtcc
    14341 agatcccgtc gcacaggaac gttgtgtctc agcatgatta gcctccccaa tgcccacggg
    14401 cgctggaaga acgaccatag ccgccgcgcg aaacggttga cgcttttttg gtggtaaagc
    14461 cgcctgaaga gtaagactct tttttgccag aaccggagcg ccaggagtaa tcaccggaag
    14521 tgttgcgcca gacggggcgg gaagcaaatg aggatccgcc gctgttatag acgaacggct
    14581 catcgcgatc tttacgaata acccggctta acaaaaatcc agaaacgacc gggatccaac
    14641 tctgttcaac attgtcgtag tagcaatttt cgtacttaga ctgacaattc tgctgagtca
    14701 tattcttcgg aacatcggca taaaatgccg ctttggcgtt gttccagcca cgcgcgcaaa
    14761 tgtcggcatt attaccgtca tcaatacaat cctgcactgt cgaataaaac gttccgtcgc
    14821 cgtcgttatc gacatcgcca ctatcgccac aacctttcaa tacaaaaaaa gcggcaccgc
    14881 ccattagagc caacgttaag tatttttgtg cgtagcgatt gcgacgcggt tcaaaaggat
    14941 tattcggtct accaatgcga ctgatcgctc cgtgaccaat tttactgtta tttcttgatt
    15001 tgcgttttct ggccatgatc gtccattacc atgtcataca agctgcgtta aaaataccgc
    15061 cagccagcgc ggcggttccc ataaacattc ctgctgcggt attgtgatta ataatttttt
    15121 cgcttaatgc gggcatataa agtctgacac cagcaaaaac gagtaactga atcaccagtg
    15181 caatcccgcc ccaggcgaaa tagtctggaa tactcaccgc attgattgcc gcactggata
    15241 atgggataac gtaacctaac aatgtaccac tgaacgccag tgaagccgcg gtattattgt
    15301 ttttgattaa ctgccattcg ttgtgcggtg taattttaga atagataaaa agaaaaataa
    15361 tcaccatagc cacgccaata aaaaaatagg cgctaaaggc aagaagtgaa tccagtatat
    15421 gcattgtaaa cgtccttgta attaaccaat aatatgaagt gatgtcagcg ggatatcgat
    15481 acccaacgcg cgcgaaaata accactctgg ctcgccgaga tcgttaaaag attcctcacc
    15541 atttaacagc aaatattcgt aagtatcttc ggttacttgc cgctggtagc ccatggtaaa
    15601 attatggacc tcccatttgg catggttttg attttctact ttttccagca tgtagacggg
    15661 ttcgatattt cccggttctt cgctgttaaa aaatcgctgc cagcgttttt cctgccagtt
    15721 taagatcatt gcccccatca ctttggcgtt gatcgcctcg cgccagtgac tctctttact
    15781 gataccgtag ctctcttcat agacaaagag cttgatatca tcaatgtcat caatatcttc
    15841 gccgccggtg gtattgattt gcagaaattc atcgcctgaa gtgtagtagc ggaaaatctg
    15901 actaccgccg cccagatcga tgtggctgac agcggctacc gtaaattcct cacccggcag
    15961 cgcaatcagc aattcatctt ccagcaaacg aaacgctaac gtgtcgagcg taaagccact
    16021 attgagatga agtcccagcg gaccgcgagc gatagcaggc ttattatcct tgccaaacag
    16081 acgctggaaa aaaccagaca tattttatcc ttaaaatagt tgccagcctt ttagcgaggc
    16141 tggcaaaaaa attactcgcc ctgttggcgt tgcagtcttg ctaatacatc ctgggcgcta
    16201 cttttattgc taccgccaat tccggcttcc gccagctttt catcaagatc gcgaccgtct
    16261 gcgacttttt ccaactgtgc agcagcatcc aggcgagcct gacgttcggc ctgacgcgtt
    16321 tgcaggcgtt ttaaggattc tgccgctgtc gaaacgctgg aagatgcgcc aacggtagag
    16381 gttgttacag cctgttgtgc acgctgcatg gcttcagtgg ctttaacgac ttccatttgc
    16441 tgctcaaact gagcgatacg ctgcgctgtc gctgtaactg ctttttccac gccatcacgg
    16501 gagacttcca gattcgacaa cacttgctct tcagcggtaa tgagattctc aaggcgcgcg
    16561 atttcttcag caacttcgtt aatcaacgac ggattaacgt tcttgcttaa cgcttccagc
    16621 gcacgggctt ccagactggc tttgcgctca cgtaaatctt tcagcttatc gtgacttaat
    16681 ttcacccgcg ccagcagatc aacgcgagat tttccggctt tatcgagttc agctttagcg
    16741 tcacgaatat gctgttccag catgcgcacg ccctgggttt cttcaatgga ttcttccgcc
    16801 tgggagataa acgatttccc cagcgtaaat aaacttttta aaattcccat aaatacccgt
    16861 ccttgtaatt agtgtgaata ttcttctgta atttcagcca gatccaatgc gttatctacc
    16921 agcgaggtta tctcaagcag gatatcttca agagaagatt taagcgataa cgccccgaaa
    16981 acaatgtaat actcttcctg ttgcacgctg gagattccta ccgatgacaa cggcatcatc
    17041 ttctgatttc ttaataagaa ggtattaaat tcatctggat tgctgatact acttaccgga
    17101 caaataaagg tttcgataat catttggcgg gaagtaaaaa gaatattgat ttgcaaatcg
    17161 ccataatcat tcattttaat aattaatgcg ctctcgctat ttgtcacatc aatattttgt
    17221 tcaggtacag tttgcagcgc cgtcgccagc gccaacggat tccatgtcat atattactcc
    17281 atatagatta agtttaaata ttaataaaat gaatatttgc aatacgtaat tatcttacca
    17341 gctatagaca aaaaaaaacc atccaaatct ggatggcttt tcataattct gagaaattag
    17401 ctgcgctggc gcaccgcttc aaataagcaa attccggtcg caaccgaaac gttcagggaa
    17461 gaaacgcttc cagccatcgg gatgctgatc aactcatcgc aatgttcacg agtcaggcga
    17521 cgcatacctt caccttccgc acccatcacc agcgccaggc gtccggtcat tttgctctga
    17581 tagagtgtat gatccgcctc gcctgccgta ccgacgatcc agatattctc ttcctgcaac
    17641 atacgcatgg tgcgcgcaag gttagtcacc cgaatcagcg gaacgctttc tgccgcgccg
    17701 caggctactt ttttcgccgt agcgttgagc tgtgcggagc gatctttcgg cacaatcacc
    17761 gcatgaacgc ctgcggcgtc cgcactacgc aggcacgcgc cgaggttgtg cggatcggtt
    17821 acgccgtcga ggatcagcag gaacggttga tcgagcgaag cgatcagatc cggcagatcg
    17881 ttttcctgat actgacgtcc tggcttcacg cgggcgataa tgccctgatg cacggcaccg
    17941 tcgcttttct cgtcgagata ttggcggttt gccaactgga taaccacgcc ctgagactca
    18001 agggcgtgaa tcagcggtaa cagacgttta tcttcacggc cttttaaaat aaagacttcc
    18061 tgaaaacgtt caggggcgcg ctccagcagg gcctgcactg cgtggatgcc gtaaatcatt
    18121 tcgctcatta atgtactcgt tgtttacgtt ttgcctgatg cactacgttt atccggtcta
    18181 catgatccct gcaatatatt gaatttgcgt gattatgtag gccggataag gcgttcacgc
    18241 cgcatccggc atgaacaaag cgcattttgt cagcaatcta accctcttct gtttgaagag
    18301 ggtaatgatc actctgccac ttttttcttc gccgcacgct tcgctttggt cgctgcagct
    18361 attttctgcg ttttcgccga tggctttttc gcttttctcg cgtctttctt cgctgctttc
    18421 ggcttcgttt ttttctcacc gcggaaggcg ctgtctggct caaagtttac ctttttaccg
    18481 acctgacgac gcttgccgcc ttttttgcct gcatcgcctt ttttcgcttt ctcgcgcgcc
    18541 gttttaccga cgttgcgcgg tgcgcgttcg ctggagatca ggctaaagtc gattttgcgc
    18601 tcgtccatat taaccgcttc gacgcgaact tccacgcgat cgcccaggcg ataagtctgg
    18661 ccgctggatt cacccatcag gcgttgccct acctggtcaa agcgatagta gtcattgtcc
    18721 agcgaagaga catggaccag cccatcaatg aacaagtcgt ccagacggac gaagaagcca
    18781 aagccagtga cgctggaaat tacgccttta aagacgttac ctacctggtc gagcatgaag
    18841 tcacacttca gccagtcagc cacatcgcgc gttgcttcgt cggcacgacg ttccgccatc
    18901 gaacagtgct gacccagttg cagcatctct tccatcgaat aatggtagcc accggtttca
    18961 gtggtgttgc cctgatgccc ctgctctttc gccaacagat acttaatggc gcggtgcagc
    19021 gtcaggtcag gataacgacg aatcggcgaa gtaaagtgcg cataggactg caatgccagg
    19081 ccaaagtgac cacggttttc cggatcgtaa atcgcctgtt tcatcgagcg cagcagcatg
    19141 gtttgcagca tttctgcatc aggacggtcg gcaaccgact ccagcagctc cgcgtagtcg
    19201 cgtggttccg gcttgttacc gcccggcagt tccagcccca gctctgccag cactgaacgg
    19261 aaagaggtaa tcgcttcggt gctcggcttg tcgtgaatac ggaacagtgc cggttctttc
    19321 gctttctcaa cgaaacgcgc cgccgagata ttcgccagaa tcatgcactc ttcaattaac
    19381 ttgtgtgcgt cgttacgctg agtctgttcg atacgttcaa tacggcgttc agcgttgaaa
    19441 atgaacttcg cttcttcgct ctcaaatgag atcccgccgc gttcttcacg ggctttatcc
    19501 agcactttat agaggttatg caactcttcg agatgcttaa ccagcggggc gtactgctcg
    19561 cgcagatcct gatcgccctg cagaatatgc cagactttgg tgtaggtcag acgcgcgtga
    19621 gagctcatca ccgcttcgta gaatttgtag ccagtcaggc ggcctttcga cgaaaccgtc
    19681 atctcgcaca ccatacacag gcggtctacc tgcggattga gcgagcacag accgttagag
    19741 agcacttccg gcagcatcgg gataacctgc gaagggaagt acaccgacgt gccacggtta
    19801 cgcgcttctc tgtccagcgg cgttggcgga cgcacatagt agctgacgtc ggcaatcgcg
    19861 acccataaac gccagccgcc gccgcgtttt ttctcgcagt aaactgcatc gtcaaagtca
    19921 cgggcgtctt cgccatcaat ggtgaccagc ggtaaatcgc gcaaatcgac acggcccgct
    19981 tttgcttctt ccggcacttc ttctttcaac ccggcaacct gttgctcaac agcctgcggc
    20041 cagatgtacg gaatttcatg ggtacgcaga gcgatatcaa ccgccatgcc ggtgcccata
    20101 ttgtcgccca gcacttcgac gattttaccc accgctttgg tacggcgagt cgggcgttgg
    20161 gtcagttcaa caaccaccac aaagcccatc cgcgcgccca tgatctgatc gggcgggatt
    20221 aaaatatcga agctcagacg gctgtcgtca ggaaccacaa agccgacgcc cgcttcggta
    20281 aagtagcggc caacaatctg gctggttttt ggcaccagta cgcggacaat acgcgcttca
    20341 cgacgacctt tacggtcagc gcccagcggc tgcgccagca cctgatcgcc atgaatgcag
    20401 gttttcatct gctcgctgga gagatacaaa tcatctttac gcccttcaac ccgcagaaag
    20461 ccgtagccat cacggtggcc aataacggta cctttcacca ggtcgaggcg ttccggcagc
    20521 gcatagcact gacgacgagt gaagaccagt tgaccatcgc gctccatcgc gcgcaggcgg
    20581 cgacgcaggc cttcaagctg ctcttcgcct tcaatgtgca gttctaccgc cagctcatca
    20641 cggctggccg gtttttcacg tttggttaaa tgttcgagga taaattccct actagggatg
    20701 ggattcgcgt atttttcagc ttcgcgttcc tggaaaggat cttgtgacat ctcggttcct
    20761 ccgttgtcat ctctgatgaa gattttcgtc actccaccag caataattta taaagcggtt
    20821 gattctcttc aaccaaatcg gcaagcgtgt agttatccag ttccgtaaga aaactttgca
    20881 cggccttaga aagcgcctgt ttcaaccgac aggcaggtgt aatgtggcaa aactcactgc
    20941 tgcaattcac cagcgataag ggctccagct cgcgcaccac atcaccaata cgtatcgcac
    21001 tcgccgattt acccaggcga atgccgccat tttttccacg aacagcagtc acgtagccgg
    21061 cacgactaag ttgattgatt attttgacca tatgattacg ggagacgccg tagacgtcag
    21121 tcacttcaga aatactggtc atccgccctt ctggcaatga cgccatgtag atcagcgcac
    21181 gtaatccgta atcagtgaaa ctcgttaact gcacatcaac ctcaaaaggg aaatcgggaa
    21241 aaaatacatt tatattgatg ataaaccagc cacaagctgt gtcgctaatt tatttcagtt
    21301 cggggaagga aaaaagcgag gatttaacac tgtgtcggat agcgtaatgg cttaaccgac
    21361 acagcaatgt atcacactgt tagcccggca ggcaaaatat ctgccaggcg taccagaatt
    21421 acgcgtcgaa cggatcgcgc agaatcatgg tttcagtacg atccggaccg gtagagatga
    21481 tatcgatcgg cacaccagtc agctcttcaa tacgcttgat gtagttcagc gccgcctgcg
    21541 gcagaccgct acgatctttc acgccgaagg tggattcaga ccagcccggc atggtttcgt
    21601 aaatcggctc tacacctttc cagtcgtcag ctgccagcgg agtggtagtc acttcgcgac
    21661 catccggcat acggtaagcc acgcagagtt taacctcttt caggccatcc agaacgtcca
    21721 gtttagtcag gcagaagcca gacagggagt tcagctgtac cgcacgacga acggcaacgg
    21781 tgtccagcca gccggtacga cgacgacgcc ccgtagttgc gccgaattcg ttaccctgct
    21841 tgcagaggaa ctcgccagtt tcatcaaaca attcagtcgg gaacggacct gcacccacac
    21901 gagtggagta agctttgagg atacccagaa cgtaatcaac ataacgcggg cccaggccgg
    21961 aaccggtcgc cacgccacca gcagtggtgt tggaagaagt tacgtacgga taagtaccgt
    22021 ggtcgatatc cagcagcgta ccctgcgcac cttcgaacat gacgaaatcg ccacgctgac
    22081 gcgcctggtc gagcaggtca gaaacgtcaa ccaccataga agtcaggatg tcggcaacag
    22141 ccatcgtatc atccagaact ttctggtaat caaccgcttc agctttgtag tagttaacca
    22201 actggaagtt gtgatattcc atcacttctt tcagtttttc agcgaaggtt tctttgtcga
    22261 aaaggtcgcc aacacgcaga ccgcgacgtg ccactttatc ttcataagca ggcccgatac
    22321 cacgaccggt ggtgccgatc gctttcgcgc cacgcgcttt ctcacgcgcg ttatccagcg
    22381 caacgtgata atcaaggatc agcggacatg cttcagacag cagcagacgc tcacgaacgg
    22441 ggatgccacg gtcttccagt tctttcatct ctttcatcag cgcggccgga gacagcacaa
    22501 caccgttacc gatgatgctg gttacattct cgcggagaat acctgatgga ataagatgga
    22561 gaacggtttt ttcaccgttg attacgagag tatggcctgc gttgtgaccg ccctggtagc
    22621 gtacaacata tttagcccgt tcagtcagaa gatcgacgat cttaccttta ccttcgtcac
    22681 cccattgggt gcccagtacg acgacgttgt tacccatttt tcaaaatcac cgtttgctta
    22741 aaaatggatt ctaccatcgc tttttcagag ttacagcact ttttgcactc aaaaatgacc
    22801 tcgatcagtc tgttttttgt tcagccaatc gttttcctca acatgtagta gaccacaacg
    22861 cccgcaacca caagtccacc gccaaaacga cgtaaaatat tatcgggcaa attggtcatc
    22921 gcagagatca tcttcttcca tgccttcggg taaagcatcg gccctaaacc ttccagtacc
    22981 aaaaccaggg caagcgccag ccagattgtc gaattcattc attatcctta tagaaaaaga
    23041 aaaccaccga catccctgag gatgcggtgg ctttattgac ctgtaccgca gtcgttatat
    23101 taacgcgttg cggaagtcgg cgtcttcatg tagcggaaga aatcgctatc cgggctcatg
    23161 accatcacgt cctgattgcc agagaagctg ttctcataag cacgcaggct acggatgaat
    23221 gcgtagaagt ccggatcttt actgaatgca tcagcaaaca gtttggctgc ttcggcatcg
    23281 ccttcaccac gcatgatgcg gccctgacgc tcagcttctg ccagcgttct ggtcacttca
    23341 tagtcggcag tcgcgcgcag tttttccgct tcttcctgac cttgtgaacg gtgacgacgc
    23401 gctaccgctt cacgctcggc gcgcatacgg ttgtagatcg cttcagacac ttcggtcggc
    23461 aggttgatct gcttgatacg cacatcgaca acttcaatac ccagcgccgc catactgttc
    23521 gggttgatga ccggaacttt gcccttcgtc tcagccgtta cgcgctctgc cgcttcggca
    23581 atggcgttat ctgctgccgg ggtagtaact tcatcttctg tacccgcaga accggagttc
    23641 agcgcgtcac gtacttcgag ggtcagacga ccacgggaat cggtgacgat gtctttcacg
    23701 tccaggcgac caatttcaga acgcagacgg tcagagaact tacgtttcaa cagcacttcc
    23761 gcttgcgaaa tgtcgccacc acccgttgcc aggtagtaac ggctgaaatc gctgatgcgc
    23821 catttgatgt aagaatcgac gatcaggtct ttcttctctt tggtcacaaa gcggtcggcc
    23881 tggttgtcca tggtctgaat acgtgcgtcg agcattttca ccgtttcaat gaacggtatc
    23941 ttgaaatgca gaccaggctc ataaaccaaa ggtttgttgt catcgtcacg cagtacctta
    24001 ccaaaacgca gcgtaatacc gcgctcacct tctttgacga caaagacaga catgtaaagc
    24061 actaccagca cgatgatgat aatcgcgata actgacttac gcatcgttat tccccctgac
    24121 gctggtagtc gttacgctgc gcgttggcgc ggcgttggtc cataatatcg ccctgactgg
    24181 tggacgacgt gttgcttgct ccactggttg tggaggaaga ggctggcggc aaacgcagca
    24241 gattgctggc accgttatcg ctcttcgccg caggggcgtt accacctttc agcatctggt
    24301 ctaacggcag aaccatcagg ttgccacctt tatcgttaac cagcactttg cgggtgttac
    24361 ccaacacttt ttccatcgtc tcgatataca gacgctcgcg agtaatttcc ggcgcggctt
    24421 tatattccgg cagaagttta gcaaagcgcg ccacttcacc ctgagcttcc aggatggtct
    24481 gggccttgta cgcacgcgcc tcttcgagga tacgttgcgc ctgaccgttc gcacgcggct
    24541 gaacttcgtt ggtatacgct tctgcttcac gaatgtattg ctgttcgttt tcacgcgcgg
    24601 caatcgcatc gtcaaacgcc gcttttactt cttccggcgg acgagcagcc tggaagttga
    24661 cgtccagcag agtgatgccc atgtcatacg gacgaatcgt ctcttccagt tcgcgctggg
    24721 tatcgctacg aatcacggta cgaccttccg tcagaatgcg gtccatggtg tatttaccga
    24781 taaccccgcg cagggcgctg tcggtagcct gacgcaggct gtcatccggg ctggtcacgc
    24841 tatacagata tttttccgga ttggtaacgc ggtactgcac gttcatctca acgcgcacta
    24901 cgttctcgtc cgacgtcagc atcacaccgg aagcggccag ttcacgcacg gcttccacgt
    24961 ttaccggttt gacttcgtcg ataaacgtcg gtttccagtt cagacccggc tcaaccagat
    25021 ggctgaattt accaaagcgt gttaccacgc cgcgttcggc ttctttaatg gtatagaaac
    25081 cactggccgc ccagataatg acaatcgctg ccgctgcgat ggtaacgaca cgaccgccaa
    25141 gctgcgggcg cgggccttgc gatgaactgc caccgccaga tccggtgcct ttaccgccgc
    25201 ccagaccacc gagctttttg ctcagtttgc ggaagatatc atctaaatca ggtggccctt
    25261 gatcgcgacc gcctttgttt ccatttccct cagagttgcc gccaggtttg ctgcttcccc
    25321 acgggtcgcg gtcttgtccg ttattaccgg gctgattcca cgccatgttt gtgctccata
    25381 tttgttatgc ggtgatccct gttgtctttc gacgccagag gatatgactc cacgcttcag
    25441 acgctacgcc gttagatcag gtaatcgatc aacgccggtt cttgtttaca gaggcgacgc
    25501 cagtcaacga tcggcatacg aacttgcaga cttacgctgc cgtcctcctc catccactct
    25561 ttttctattg cctgaagctg ataaaaacga cttctcagac gcccttcctg cggtggcaga
    25621 cgcaatgtat gctgcgccac ctcgccggaa agccgctccg tcaaagcctg aaaaagctgt
    25681 ggtatccccg ctccggtctg tgcggaaagc cagacacgga tcggtttgtt ctcttcgtcc
    25741 cgatcaatac gcggttcgaa atcttccagc atatcgatct tgttcatcac cagcagggtt
    25801 gggatctcgt gagcgtcgat ctcttcaaga accgtattca ccgcttcgat gttttcttgt
    25861 acacgcacat ccgccgcatc aatcacatgc agcagcaatg tggcttgccg cgtctcttgc
    25921 aacgtggctt taaacgccgc caccagatcg tgcggcaggt ggcgaataaa ccctacggta
    25981 tctgcaagta cggtttcacc gacatctgca acgtcaatac gccgcaacgt cgggtcgagg
    26041 gtggcaaaca actggtctgc cgcgtagacc cgcgcttcgg tgatgcgatt gaaaagggta
    26101 gatttaccgg cgttggtata tcccaccagc gaaacagtag gaacgtcggc tttgatacgc
    26161 gattgccgcc cctgctcacg ctgcttttca actctttcca ggcgtgactg tatctgcacg
    26221 atgcgattac gcaacaaacg acggtcggtt tcgagctggg tttcacccgg accacgcaaa
    26281 cctatcccgc ctttctgtct ttcaaggtgg gtccagccac gcaccaggcg cgtagccaga
    26341 tggcgcagct gcgccagctc aacctgcaac ttaccctcat gggtacgcgc acgttgggcg
    26401 aaaatatcta aaataaggcc ggtgcggtcg ataacacgac actcgcacaa acgctccagg
    26461 ttacgctctt gcgccgggct cagggcatgg tcaaaaagaa cgaccgaagc acccgtcgct
    26521 ttgacagctt ccgcaatttc aactgcttta ccttcaccta caaaatactt tgggtgcggc
    26581 gctttacggc taccggtaat cacctgcaat gcttcgacac cggcggaaga gaccagagat
    26641 tcaaactcct ggaggtcttc catatctttg tcttgcgtaa aatagatgtg taccagtacc
    26701 gcctgctcac cagcatcata acggtcaaac aagcgtataa ccctctaaat agatcagcgg
    26761 ggaacgcagg atcgctggct ccccgtgtaa aaaaaacagc ccgaaacctt attcggtttc
    26821 ttcgctgtcc tgttgcgcgg aagtattctg cgcgctgcta ccatgatggt agttactgct
    26881 ggtaccgcca ccggcgttgt tactgtgatg agaaaccggg cgagacggga caacagtaga
    26941 aatcgcgtgc ttgtaaacca tctggctgac cgtgtttttc aacaggatca cgaactgatc
    27001 aaaagactcg atttgccctt gcagcttaat accattcacc aaataaatag aaactggaac
    27061 acgttcccga cgcagtgcgt tcaggaacgg atcttgtaaa gattgcccct tagccattct
    27121 ctcttttcct tatatgctta tttgtacttt gaacctttcg attctgaaaa aattgcgcac
    27181 gatacgtctc aattgtacac attcagcctg cgatagcacc aacaacctgt aatacttcgt
    27241 cacgcgcctg ttctggtttt tcactatcaa gccagtgaac cccttcccaa ccacgcagcc
    27301 aggttatctg ccgcttcgcc aactgtctcg tggcgcaaac acctcgataa accatttcat
    27361 cgtatgagat ttcgccttca aggtaagacc acatctggcg ataacccacg caacgaatgg
    27421 aaggcaaatc cgtatgcaaa tctcctcggg caaaaagcgc ccggacttct gcttcaaaac
    27481 ctgaagccaa catctgatga aaacgctgct caatgcgttg atggagcagt tcacggctcg
    27541 ccggggcgat ggcgaactga tgcacctgat acggtagagc gtctcctgac gtttgcgtca
    27601 gttccgttaa agttttaccc gaaatgaaaa aaacttccag tgcccgggaa agcctttgtg
    27661 gatcatttgg atgaatcctt gctgccgcaa ccggatcgat ctcctgaagt tgacgatgca
    27721 atgactccca accttgctct gccgcctgtt gctcaattct ggcccgtact tccgggtctg
    27781 ccgacggtag cggcgacaac ccttccagca atgccttgaa atacaacatc gtaccgccca
    27841 ctaacagtgg gatccgcccc gccgcggtga tatcggccat ttccgccagc gcatcgcggc
    27901 gaaaatcagc agccgagtaa gcctgcgacg gatcgcgaat atccagcaat cggtgcggcg
    27961 cggcgagtaa ctcttcagcg ttcggcttcg ccgtcccgat atccatccct ttgtaaataa
    28021 gggcagaatc aacgcttatc aactctactg gtaaaatttt acgcagctca atggctaacg
    28081 ccgttttacc ggaggccgtc ggccccatca aaaaaatcgc cttaggcagg ctcgccttac
    28141 tgatatcact catctttcag ggcttttatc gccggatgta aatcaacaga ttgtaacaga
    28201 ccacccggcg gcgttttcac aagttgcgga cataaccgtt ccacgtccgc cagcagggtt
    28261 atggcctgtg ccatggacca ctgcgcatgt tcgctcatca gatttcgtgc aatccactgc
    28321 gcaatattgc caggttcgaa tacggactgc ttcgccaggt agcctatcag ttcaggaatc
    28381 aagatttgta aattttgttg gcgtaagggt aaaggcacgg ccctgatggt cacatgctgt
    28441 gcatctgact ggaaatcaat acccaattcc gccagggcag actgcgcttt ttctaatgcc
    28501 gatttttctt cgccagaaac ttttagccgc aacggaataa gcagcggctg ggcgcaaacg
    28561 ggcgcttcac ccggcgtcag ttgcgcctga cgcagccaac gttctgccac tggcaaggct
    28621 aaaagtgaaa tgttgccgtc gcgctccagc aacgcacagt cggaatggac gatagtcagt
    28681 acccgaccaa aactctgact gttcgccgca agtgcaggtt cctgcggttc tggcgctttt
    28741 ggtttttgca tcggcgcggg cgtttgcaaa agctggcgat acacttcacc ttgctgtttc
    28801 tggtaacctg gctgcgcatt cggccagggg gcagccggac gactgcctga ggcgggcgca
    28861 ggagtgtaac gcggagctac cggctcacga gctgccggtt cagcaaaatg attgcgcccc
    28921 gccaccacgc ggttttccgg aatagcacgc ggtgcaggtt ggggttcatc gtccagcggt
    28981 agcggcgttt ccagctgctg ttgtagcacg ctcagcacgc cctgatagat aaagtcatgc
    29041 accagacgcg actgatgaaa acgcacttcg tgtttggcgg ggtgcacgtt aacgtccacc
    29101 tgatgcgggt cgatctccag atacaacaca aatgccggtt gctgatcggc ccccagtttg
    29161 tcttcgcagg cctggcggat cgcgtgattg atcaggcgat cgcgcatcat gcgaccgttc
    29221 acgtagcaat actgaatttc tgccagggcg ggcgtggtgt gattcggatc ggccacccag
    29281 ccgcgcaggg tgagatcgcc gtgttgccat tcaatcgcca gcgcttgttc gagaaaagcg
    29341 gtgccgcaaa tcgcgcctaa gcgccgttct ttttgcccgc cttccggcac tgcacggtac
    29401 tgacgcacaa ttttaccgtt atgcgacagg ttgatcgtga cgtcgaaacg cgccagcgca
    29461 atgcggcgga tgatctcatc aatgtggtta aattcggttt tctcggtgcg caggaatttg
    29521 cgccgcgccg gggtgttgta gaacagatcc agcacctcca gcgtcgtccc cacaggatgc
    29581 gccgccggtt ttaccgtcac gttcatatcg cgcccttcgg cataggcctg ccaggcttcc
    29641 tgctgttctg cggtgcgtga agtgagcgtc aggcgggaaa ccgaactgat actcgccagc
    29701 gcctcaccgc gaaagcccag gctgataatg gcttcgagat cgtccagaga ggcgatttta
    29761 ctggtggcat gacgagccag cgccagcgcc agctcatctt ttttgatacc gcagccgtta
    29821 tcacgaatgc ggataagttt cgccccaccg cgttcgatat caatatcgat acgcgtcgca
    29881 cctgcatcga ggctgttttc caccagttct ttgactaccg acgcaggtcg ctcgaccacc
    29941 tcacctgcgg caatctggtt cgccagttgt ggcggtaaga cctgaattgg catcaatcgt
    30001 ccttagtttg gcagcgtgcg atctggcgtc gtcaccgtac tggcagtttg tgccgttgca
    30061 ccctgcggcg cagattgcat cggatgcgca aggaaataat tgcgcaggcc tttgtaaatg
    30121 gcttctgcca gctgttgttg gtaatcgtcg ctcgccagca aacgttcttc gctgttgttg
    30181 ctgataaaac cggtttcgac cagtactgat gggatatccg gcgaacgcag aacgccaagg
    30241 ctggcgtgtt ctggtcgacg tttatgaatt tcgccaatgc gttgcaactg actgatcata
    30301 ctggtcgcta catcataccc tacccgctgg gaatgaccga actgtaaatc cagcaccgcc
    30361 tggcttaaat aggggtcaga ctgactgttc gccagcacat cgcccgctcc gcccagtagc
    30421 tctgactgtt tctcgtgctg ctccagccag ctggccattt cgctgttggc gcgacggtta
    30481 gagagcaccc atacggaagc gccagtcgca ctgcggttcg gtgcggcatc agcgtgaatc
    30541 gacacgagga aattggcgtt ttgcttacgt gccacatcgc tgcgccccat caccgagata
    30601 aagtaatccc cgtcacgggt taaaacgcct ttaaacatcg gatcgtcatt gagcaaagta
    30661 cgcaatttac gcgcgatggc gatggtgaca tttttctccc gcgtaccacc ggggccgata
    30721 gcgccagggt cctgaccgcc gtgtccggca tcaatagcga tgataatttt atcgccagtg
    30781 ttagccgtcg cgcgcgctgc cggacgcgtt accgtattac tgctgataac acccgtagtg
    30841 cggttacttt ccgttttaaa cggattgcgc gccggttcgc tgacgcgcgg tgcgacaacc
    30901 gtaggcgttt caacgcgttt cgcaaccaca ggcggcggag gaggcggtgg cggcgcatcg
    30961 gcgttaatcg taaagacgac agtgtaattg ctgccattct gccgcttcac cgcttcggtt
    31021 ttaccatttt cggtaagatc aaccaccagc cgtagcgttt gtgcatcttt aggcgttccg
    31081 gagcgaatcg ccttcaccag attattaccg ctgaacaaca acggcagtcc ctgaatcacg
    31141 cccgtttgtt tgatatcgag cgccacggtg cgtttgcttt gatggctaaa cgcataatca
    31201 ggatcgccaa taaaactcaa cgttatccgc gcctgttggt tgccgttaga aacctgaata
    31261 tcagagagcg tcgcggcacc cacctgcgcg cacagcagca gcaacgtcgc taccaaccaa
    31321 tttctgatgc gatacatcat cccgccacct ttcaaaggtt aaccggctaa acgcgccagc
    31381 aacaattcac ccgcagagga aaccgcactc acgcgcgcct cacggccttg tgcctgataa
    31441 tcaatgtgta tttcgacatc cgggtcagga agaacacctg taccttgttg tggccactcc
    31501 accaggcaga tggcatcgtt ggcaaaataa tcgcggatcc ccataaactc cagctcctcg
    31561 ggatcggcaa ggcggtacaa atcaaagtga taaaccatta agttgtcgag cgtatagggt
    31621 tcgaccagcg tataagtggg gcttttgaca ttaccctgat ggcccaaagc ctgtaaaaag
    31681 ccccggctaa aggtggtttt tcctgcgcct aaatcgccat acagatagat tacggttgcg
    31741 ccatcgcagg ctttcgctac ccgctcgccc aggtctaatg ttgcctgctc atcagggagc
    31801 ggaattactc gattcatcat ggtttttatc agtcacttcc gggttaacaa tacgctgtag
    31861 cgtggaaaag agatcggttg ccagcatccc gcgcgttcca aaacgcgccg ccagtacgtc
    31921 agctgccgca ccgtgcgcga cacagcctgc acaggctgca tcatacggcg acagtttttg
    31981 cccaagcaat gcgccaataa taccagagag cacatcgccc atgccgccgc ttgccatgcc
    32041 tgcatttccg gcatcaataa tgcctaaagc gtcaggatgg gcggcgacca cggttccggc
    32101 acctttcagc accgctacgc cgccataacg ttgtaccaga cgtttggcgc aatgtaagcg
    32161 gtcactttca atttcagcga cggaacagcc taacaaccgt gcggcctcgc caggatgcgg
    32221 cgtgatcacg cgattgtgac gcttatcggg attgattgcc agcaggttca atgcatcggc
    32281 atcccacaac atcggtttgc gaaaattctc aactttttgc agggcttttt tcccccactc
    32341 ttgctggccc agaccgggac caatcaccac cacatcggcc cattccaggc tttcggtaag
    32401 agagtccata gtcagttcat gcaccatcaa ttctggtcgt gcagtcagca gcggcgcaat
    32461 gttctcactg cgggtcagta ctcggactaa accagcacca gcacgtagcg ccgcttcccc
    32521 cgtcatacga atagcccccg ccgtgccgtg atcgccaccg ataatcacca gccgcccgtg
    32581 atcgccttta tgcgaagtcg ggcgacgcgg tttcagccag tgagaaagtt gttctgccga
    32641 aaaccgctga attttcgtct cctgacctgc cagccagcta tccagcccca gtgagtcaaa
    32701 atgcagttgt cctgtaacat cccgcgcttt tccagtgagc aagcctggtt tcagcgcaat
    32761 aaaagtgatg gtgtgatcgg cgttgatcac tgcgcctggc gtagcgccgg tttcagccag
    32821 caggccggaa gggatatcaa ccgccacaat cggcgcagga tgggaattag cgtggtcgat
    32881 taactggcta atggattcgc ggggtgcttg ctgcaagccg gtaccgagca gcgcatcaac
    32941 aatcagagct accgactcgg gccagacaat attcgaagca tggatctcgc ctcccgcgtt
    33001 taaccatgcc tcgcgtgcca gcgcggcctc ttccggcaac ggtttgtcgc tctcctgggc
    33061 caacaacgtg acctcaatgc cgaccgcttt ggccagtcgc gcgaccacgt agccatcgcc
    33121 gccgttatta ccatgaccgc acaacaccag ccagtggcgg gcgtcaggat acgccgaacg
    33181 acacacctgg aatgcggcct cgccagcgcg aagcatcagc tcatagagtg tgagccccag
    33241 cgcatctgcc gcctcgcgtt ctccgcggcg gatatcgtcg gcgtgccaga cggtgtgtgg
    33301 tatacttacg gggtttttct tcattgtatg gtccgtcatg tcagagcccc tcgatctcaa
    33361 tcagttagcg caaaaaatta aacagtgggg gctggaactg ggctttcagc aggtaggtat
    33421 taccgatacc gatctcagcg agtccgagcc caaactgcaa gcatggctgg acaaacaata
    33481 ccacggcgaa atggactgga tggcacgtca cggtatgctg cgcgctcgcc cccatgagtt
    33541 attgcccggt acgctgcgcg tgatcagcgt gcggatgaat taccttcctg ctaacgccgc
    33601 atttgccagc acgctgaaaa accccaaact cggctatgtt agccgttatg cgctgggccg
    33661 tgactatcac aaacttctgc gcaaccgact caaaaagctg ggcgagatga ttcagcaaca
    33721 ttgtgtttcg ctgaatttta gaccgtttgt cgattctgcg cctattctcg agcgcccgtt
    33781 agctgaaaaa gctgggctcg gctggacagg taagcactca cttatcctca atcgcgaggc
    33841 cggttcgttc ttctttttag gcgaattgct ggtcgatatt ccgctgcccg tggatcaacc
    33901 agtcgaggaa ggatgcggca aatgcgtggc ctgtatgacg atttgcccga ccggtgccat
    33961 cgtcgagcca tataccgtcg atgctcgccg ctgtatctct tatctcacca tcgaactgga
    34021 aggggcgatc ccggaagagt tgcgaccgtt aatgggaaac cgtatttacg gttgcgatga
    34081 ctgccagctt atctgcccgt ggaatcgcta ttcacaactc actacagaag acgatttcag
    34141 cccgcgtaag ccgctacacg caccggaact cattgagtta ttcgcctgga gcgaagagaa
    34201 gtttttaaaa gtcacggaag gttcggcgat tcgccgtata ggtcacctgc gctggctgcg
    34261 taatatcgcc gtggcattag gcaatgcgcc ctgggatgaa acgattctgg cggcgctgga
    34321 gagtcgcaaa ggtgagcacc cacttcttga tgagcacata gcgtgggcga tggcgcagca
    34381 aatcgagagg cgaaatgcgt gcatagtcga agtgcaattg ccgaaaaaac agcgtctggt
    34441 tcgggtgatt gaaaaagggt taccgcgtga cgcctgattc atccacagct tgtgaataaa
    34501 aataaaaacg cattgcaatt caagacgaat aaaatcgtca agtgatcgac tcgacaaaac
    34561 ggaatcttaa ttcttaactt atctatcaat aggttaaaaa aatactattc accgtgcgaa
    34621 gtttctttga aattaagatt acagcctgag gctgtggata actctgttta caagagtttg
    34681 tcagagacaa caaaaaccag caataacgca tcgcgtcgct gtggatattt tattgagaga
    34741 aga
//
LOCUS       DAPGOF010000031        34675 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.031, whole genome shotgun sequence.
ACCESSION   DAPGOF010000031 DAPGOF010000000
VERSION     DAPGOF010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.031"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(153..828)
                     /locus_tag="RHV13_001322"
                     /pseudo
     CDS             complement(153..828)
                     /locus_tag="RHV13_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(973..1581)
                     /locus_tag="RHV13_001323"
     CDS             complement(973..1581)
                     /locus_tag="RHV13_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HDV1240876.1"
                     /translation="MKNRLLILSLLVSVPVFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYIFEAVGPVKYTPLKQWIAHGEKDKYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRHYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(1765..2082)
                     /gene="metJ"
                     /locus_tag="RHV13_001324"
     CDS             complement(1765..2082)
                     /gene="metJ"
                     /locus_tag="RHV13_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HDV1240877.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            2359..3519
                     /gene="metB"
                     /locus_tag="RHV13_001325"
     CDS             2359..3519
                     /gene="metB"
                     /locus_tag="RHV13_001325"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HDV1240878.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            3522..5954
                     /gene="metL"
                     /locus_tag="RHV13_001326"
     CDS             3522..5954
                     /gene="metL"
                     /locus_tag="RHV13_001326"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HDV1240879.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPTSQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYDIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            6303..7193
                     /gene="metF"
                     /locus_tag="RHV13_001327"
     CDS             6303..7193
                     /gene="metF"
                     /locus_tag="RHV13_001327"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HDV1240880.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            7522..9702
                     /gene="katG"
                     /locus_tag="RHV13_001328"
     CDS             7522..9702
                     /gene="katG"
                     /locus_tag="RHV13_001328"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HDV1240881.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            9796..10701
                     /gene="yijE"
                     /locus_tag="RHV13_001329"
     CDS             9796..10701
                     /gene="yijE"
                     /locus_tag="RHV13_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HDV1240882.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAAVFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVMALALVSRKKKEAVSVKRI"
     gene            complement(10728..11345)
                     /locus_tag="RHV13_001330"
     CDS             complement(10728..11345)
                     /locus_tag="RHV13_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HDV1240883.1"
                     /translation="MKASLVLVSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFTRHDKTRPTSKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(11620..12723)
                     /gene="gldA"
                     /locus_tag="RHV13_001331"
     CDS             complement(11620..12723)
                     /gene="gldA"
                     /locus_tag="RHV13_001331"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HDV1240884.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVAVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(12734..13396)
                     /gene="fsa"
                     /locus_tag="RHV13_001332"
     CDS             complement(12734..13396)
                     /gene="fsa"
                     /locus_tag="RHV13_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDV1240885.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(13408..15909)
                     /gene="ptsP"
                     /locus_tag="RHV13_001333"
     CDS             complement(13408..15909)
                     /gene="ptsP"
                     /locus_tag="RHV13_001333"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDV1240886.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQCLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLRQHLLAGVSEGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLTEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLIGLGLDELSMSAPSIPAAKARMAQLDSRECRQLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            16218..17297
                     /locus_tag="RHV13_001334"
     CDS             16218..17297
                     /locus_tag="RHV13_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HDV1240887.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            17312..17632
                     /locus_tag="RHV13_001335"
     CDS             17312..17632
                     /locus_tag="RHV13_001335"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HDV1240888.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            17683..19980
                     /locus_tag="RHV13_001336"
     CDS             17683..19980
                     /locus_tag="RHV13_001336"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDV1240889.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASRKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPTFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTEETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFDQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFIELSKEIQDDIIRRTAHQL"
     gene            19946..20824
                     /locus_tag="RHV13_001337"
     CDS             19946..20824
                     /locus_tag="RHV13_001337"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HDV1240890.1"
                     /translation="MTSSAGQRISCNVVETRRNDAARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLNIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            20826..21167
                     /locus_tag="RHV13_001338"
     CDS             20826..21167
                     /locus_tag="RHV13_001338"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HDV1240891.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCLLEKWGISIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(21154..22005)
                     /locus_tag="RHV13_001339"
     CDS             complement(21154..22005)
                     /locus_tag="RHV13_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV1240892.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(22220..23953)
                     /locus_tag="RHV13_001340"
     CDS             complement(22220..23953)
                     /locus_tag="RHV13_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HDV1240893.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQEVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(24135..26786)
                     /gene="ppc"
                     /locus_tag="RHV13_001341"
     CDS             complement(24135..26786)
                     /gene="ppc"
                     /locus_tag="RHV13_001341"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HDV1240894.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(27384..28535)
                     /gene="argE"
                     /locus_tag="RHV13_001342"
     CDS             complement(27384..28535)
                     /gene="argE"
                     /locus_tag="RHV13_001342"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HDV1240895.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDTLRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYDAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEFLETRFIKPTRELITQIIHHFCWH"
     gene            28689..29693
                     /gene="argC"
                     /locus_tag="RHV13_001343"
     CDS             28689..29693
                     /gene="argC"
                     /locus_tag="RHV13_001343"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HDV1240896.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            29701..30477
                     /gene="argB"
                     /locus_tag="RHV13_001344"
     CDS             29701..30477
                     /gene="argB"
                     /locus_tag="RHV13_001344"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HDV1240897.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTRLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            30538..31911
                     /gene="argH"
                     /locus_tag="RHV13_001345"
     CDS             30538..31911
                     /gene="argH"
                     /locus_tag="RHV13_001345"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HDV1240898.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FARARLG"
     gene            32179..33096
                     /gene="oxyR"
                     /locus_tag="RHV13_001346"
     CDS             32179..33096
                     /gene="oxyR"
                     /locus_tag="RHV13_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HDV1240899.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(33079..34479)
                     /gene="sthA"
                     /locus_tag="RHV13_001347"
     CDS             complement(33079..34479)
                     /gene="sthA"
                     /locus_tag="RHV13_001347"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HDV1240900.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
BASE COUNT         7830 a         8762 c         9691 g         8392 t
ORIGIN      
        1 tatgcctatg aatatgatgt aaaatataat atatgtcatt tgagaaaaaa gagcagaatg
       61 aggtaaaaat ctttggctgt gtattcagtc tggtaatcat ccaaggaaag ctcatcagaa
      121 gggtgatgac tccaattagt agaacatata ttttcgataa aagcaccgat gactttttca
      181 tgaagttcta cagaacgcga gaaacagatg gttctgcggg ccagacgttt gattcgggtt
      241 ctcagggtca gattgttacg ctcaatgtgc tgagtaaaaa tcttaccagt cagatgcttc
      301 tcctttggta actctctagc gtaactaccc cattcgtcag tggtcaacat gccgatggta
      361 aaagggtaag cagcgccagc aattcacgac aggtttcatc agtccggggg ccaaaagtgt
      421 aggccagaac accaccagtt ttcgtgttgt acacgtacca gagccagtgc tgtcgcgctt
      481 tactgccgac aaagctccat tgctgactaa gtttgcagat gagtgccaca tcagcatgag
      541 cgaccgggga tgacgttatt cttcgtggcg agagtttttt aaagttcgga tgactgtatt
      601 aatgccgatt ttcagcgtac gggcagtatc gcgaaccccg gcaccgttga aagccatttc
      661 agtgatttgg tctttaacta ccggtttacg gggctcgtaa cagtaactga gctgaaatac
      721 ccggtgacag tcacggccgc ggaatcgatc atgtcttttt gggttctgcc cgtgacggta
      781 aacctgaacg gactggcaac gggggcaatg aaggttaact gtgaccatga aacaacctta
      841 aaagcgagta ttatatcctt aattcaacta attggaggca tcaccgttta ccggagcgac
      901 agcttgcgga aaggtcgtgc aactacaaag caacaaacag actacggcgg gaattatttt
      961 tttcatcatc cattaccacg aaaacagcgg ccactctttg gcaaccgtgg tgagttgtgg
     1021 cgcgtcgaaa acggcttgtg gtgaaactac cgtttcctca agcggaatat ttttaccgta
     1081 gcgttctttg agcttcgcct ggaccagcgg gttgctgaga tcaaactctt tcaacttctg
     1141 ctgttcgccc acgcgcattc ccagcgcgtt ctggtaaacc ttccacacca cttccgaaca
     1201 gtaatggcga tcgtcgctcc atgagaagct gaaatcatac ggtttaccga gataacgttt
     1261 tgccgtttgc gtcagttttt gctgttgttc aacactcagt ccgccttcaa cgcggcgaac
     1321 aacgtatttg tccttttcac catgggcaat ccactgcttg agcggggtgt atttcaccgg
     1381 gccgaccgct tcaaaaatgt agggcttttt gttacgcatc accagcatac cggtgtggct
     1441 gtaatcggaa tgggttgcca gttggatcgc tttactttgc gatgagcgag agatttgaaa
     1501 gatgatgtcg ccagtttgcg gctgccaggc aaagacaggt acagaaacca gcaggctgag
     1561 gatcagcagc ctgtttttca tagttaaacg tccatgtata aaaagcggtg ggtcgcagac
     1621 aacgtgctcg ttgtttatgc cggatgcggc gtgaacgcct tatccggcct acaagttcgt
     1681 gcaaattcaa taaattgcaa tatgacgtag gcctgataag cgtagcgcat caggcgattc
     1741 cactccgcgc cgctcttttc cgctttagta ttcccacgtc tccgggttaa tccccatctc
     1801 acgcatgatc tcttttgccg cttccgggat ttcgtcgctg cgctctttac gcagatcggc
     1861 atcatccggc aaaggttgcc cggtaaaggc atgcagaaac gcttcgcaca gcagctcgct
     1921 gttggtagcg tgacgcaggt tgttcacctg acgacgcgtg cgttcatcgg tgaggatttt
     1981 taacacctta agaggaatgg aaaccgtaat ctttttgact tgttcactct tcttgccgtg
     2041 ctcagcgtat gggctgatat attcgccgct ccattcagcc atgagatact taatcctctt
     2101 cgtcaataaa ttgagaccag accacacaat ttatgtgggt actgaccgta aacccgcata
     2161 gtttaccgta caggcgttac cgtgacatcg tgtaatgcac ctgtcggcgt gataatgcat
     2221 ataattttaa cggctatttg ggatttgctc aatctatacg caaagaagtt tagatgtcca
     2281 gatgtattga cgtccattaa cacaatgttt actctggtgc ctgacatttc accgaccaag
     2341 cccagggaac ttcatcacat gacgcgtaaa caggccacca tcgcagtgcg tagcgggtta
     2401 aatgacgacg aacagtatgg ttgcgttgtc cctcccatcc atctttccag cacctataac
     2461 tttaccggat ttaatgaacc gcgcgcgcat gattactcgc gtcgcggcaa cccaacgcgc
     2521 gatgtggttc agcgtgcgct ggcagaactg gaaggcggtg ctggtgcagt acttactaat
     2581 accggcatgt ccgcgattca cctggtaacg accgtctttt tgaaacctgg cgatctgctg
     2641 gttgcgccgc acgactgcta cggcggtagc tatcgcctgt tcgacagcct ggcgaaacgc
     2701 ggttgctatc gcgtgttgtt tgttgatcaa ggcgatgaac aggcattacg ggcagcgctg
     2761 gcagaaaaac ccaaactggt actggtagaa agcccaagta atccattgtt acgcgtcgtg
     2821 gatattgcga aaatctgcca tctggcaagg gaagtcgggg cggtgagcgt ggtggataac
     2881 accttcttaa gcccggcatt acaaaatccg ctggcattag gtgccgatct ggtgttgcat
     2941 tcatgtacga aatatctgaa cggtcactca gacgtggtgg ccggcgtggt gattgctaaa
     3001 gacccggacg ttgtcactga acttgcctgg tgggcaaaca atattggcgt gacgggcggc
     3061 gcgtttgaca gctatctgct gctacgcggg ttgcgaacgc tggtgccgcg tatggagctg
     3121 gcccagcgta acgcgcaggc gattgtgaaa tatctgcaaa cccagccgtt ggtgaaaaaa
     3181 ctgtatcacc cgtcgctgcc ggaaaatcag gggcatgaaa ttgccgcgcg tcagcaaaaa
     3241 ggctttggcg caatgttgag ttttgaactg gatggcgatg agcagacgct gcgtcgtttc
     3301 ctgggcgggc tgtcgttgtt tacgctggcg gaatcattag ggggagtgga aagtttaatc
     3361 tctcacgccg caaccatgac acatgcaggc atggcaccag aagcgcgtgc tgccgccggg
     3421 atctccgaga cgctgctgcg tatctccacc ggtattgaag atggcgaaga tttaattgcc
     3481 gacctggaaa atggcttccg ggctgcaaac aaggggtaaa aatgagtgtg attgcgcagg
     3541 caggggcgaa aggtcgtcaa ctgcataaat ttggtggcag tagtctggct gatgtgaagt
     3601 gttatttgcg tgtcgcgggc attatggcgg agtactctca gcctgacgat atgatggtgg
     3661 tttccgccgc cggtagcacc actaaccagt tgattaactg gttgaaacta agccagaccg
     3721 atcgtctctc tgcgcatcag gttcaacaaa cgctgcgtcg ctatcagtgc gatctgatta
     3781 gcggtctgct acccgctgaa gaagccgata gcctcattag cgcttttgtc agcgatcttg
     3841 agcgcctggc ggcgttgctc gacagtggta ttaacgatgc ggtgtatgcg gaagtggtgg
     3901 gccacggtga agtgtggtcg gcgcgcctga tgtctgcggt acttaatcaa caagggctgc
     3961 cagcggcctg gcttgatgcc cgcgagtttt tacgcgctga acgcgctgcg caaccgcagg
     4021 ttgatgaagg gctttcttac ccgttgctgc aacagctact ggtacaacat ccgggtaaac
     4081 gtctggtggt gaccgggttt atcagccgta acaacgccgg tgaaacggtg ctgctggggc
     4141 gtaacggttc cgactattcc gcgacacaaa tcggtgcgct ggcgggtgtt tctcgtgtaa
     4201 ccatctggag cgacgtcgcc ggggtataca gtgccgatcc gcgtaaagtg aaagatgcct
     4261 gtctgctgcc attgctgcgt ctggatgaag ccagcgaact ggcgcgtctg gcggctcccg
     4321 ttcttcacgc ccgtacttta cagccagttt ctggcagcga aatcgacctg cagctgcgct
     4381 gtagctacac gccggatcaa ggttccacgc gcattgaacg cgtgctggcc tccggtactg
     4441 gtgcgcgtat tgtcaccagc cacgatgatg tctgcttgat tgagtttcag gtgcccacca
     4501 gccaggattt caaactggcg cataaagaga tcgaccagat cctgaaacgt gcccaggtgc
     4561 gtccactggc ggttggcgtg cataacgatc gccagttgct gcaattttgc tacacctcag
     4621 aagtggccga cagtgcgctg aaaatcctcg acgaagcggg attacctggc gaactgcgcc
     4681 tgcgtcaggg gctggcgctg gtggcgatgg tcggggcggg tgtcacccgt aacccgctgc
     4741 attgccaccg cttctggcag caactgaaag gccagccggt cgagtttacc tggcagtccg
     4801 atgacggcat cagcctggtg gccgtactgc gcaccggccc gaccgaaagc ctgattcagg
     4861 gcctgcatca gtccgtcttc cgcgcagaaa aacgcatcgg cctggtattg ttcggcaaag
     4921 gcaatatcgg ttcccgctgg ctggaactgt tcgcccgcga gcagagcacg ctttcggcgc
     4981 gtaccggctt tgaatttgtg ctggcaggcg tggtggacag ccgccgcagt ctgttgagtt
     5041 atgacgggct ggacgccagc cgcgcgttag ccttcttcaa cgatgaagcg gtcgagcagg
     5101 atgaagagtc gcttttcctg tggatgcgcg cccatccgta tgatgattta gtggtgctgg
     5161 acgttaccgc cagccagcag cttgctgatc agtatctcga tttcgccagc cacggtttcc
     5221 acgtcattag cgccaacaaa ctggcaggag caagcgacag caataaatat cgccagatcc
     5281 acgacgcctt cgaaaaaacc gggcgtcact ggctatacaa cgccaccgtt ggtgcgggct
     5341 tgccgatcaa ccataccgtg cgcgatctga tcgacagcgg cgatactatt ttgtcgatca
     5401 gcgggatctt ctccggcacg ctctcctggc tgttcctgca attcgacggt agcgtgccgt
     5461 ttaccgagct ggtggatcag gcgtggcagc agggcttaac cgagcctgat ccgcgtgacg
     5521 acctctccgg caaagacgtg atgcgcaagc tggtgattct ggcgcgtgaa gcgggttacg
     5581 acatcgaacc ggatcaggtg cgcgtggaat cgctggtgcc tgcccattgc gaaggcggca
     5641 gcatcgacca cttctttgaa aacggcgatg aacttaacga gcagatggtg caacggctgg
     5701 aagcggcccg cgaaatgggg ctggtgctgc gctacgtggc gcgtttcgat gccaatggca
     5761 aagcgcgtgt gggcgtggaa gctgtgcgtg aagaccatcc gctggcatca ctgctgccgt
     5821 gcgacaacgt ctttgccatc gaaagccgct ggtatcgcga taacccgctg gtgatccgtg
     5881 ggcctggcgc tgggcgcgac gtcaccgccg gggcgattca gtcggatatc aaccggctgg
     5941 cacagttgtt gtaatttcag aaatttaata atgcccggta ctcatgtttt cgggtctatg
     6001 gtttctaatg aaatatattg aattatcata ggattaggcc ggatgaagcg tttacgacga
     6061 atccggcaag aagcaataag tacatggtta gtttatattt gcagtccggt ttgctttgca
     6121 taccggattt tctttttctt accatcctga agttttttca tcttccctga tttttcctca
     6181 ccatcattgg tcatttttcg gttgacgccc ttcggctttt ccttcatctt tacatctgga
     6241 cgtctaaatg gatagatgtg cacaacacaa catataacta caagcgattg atgaggtaag
     6301 gtatgagctt ttttcacgcc agccagcggg atgccctgaa tcagagcctg gcagaagtcc
     6361 aggggcagat taacgtttcg ttcgagtttt tcccgccgcg taccagtgaa atggagcaga
     6421 ccctgtggaa ctccatcgat cgccttagca gcctgaaacc gaagtttgta tcggtgacct
     6481 atggcgcgaa ctccggcgag cgcgaccgta cgcacagcat tattaaaggc attaaagatc
     6541 gcactggtct ggaagcggca ccgcatctta cttgcattga tgcgacgccc gacgagctgc
     6601 gcaccattgc acgcgactac tggaataacg gtattcgtca tatcgtggcg ctgcgtggcg
     6661 atctgccgcc gggaagtggt aagccggaaa tgtatgcttc tgacctggtg acgctgttaa
     6721 aagaagtggc agatttcgat atctccgtgg cggcgtatcc ggaggttcac ccggaagcaa
     6781 aaagcgctca ggcggatttg ctcaacctga aacgcaaagt cgatgccgga gccaaccgcg
     6841 cgattactca gttcttcttc gatgtcgaaa gctacctgcg ttttcgtgac cgctgcgtat
     6901 cggcgggtat tgatgtggaa attattccgg gaattttgcc ggtctcgaac tttaaacaag
     6961 cgaagaaatt tgccgatatg accaacgtgc gtattccggc gtggatggca caaatgtttg
     7021 acgggctgga cgacgatgcc gaaacccgca aactggtggg agcgaatatt gccatggata
     7081 tggtgaagat tttaagccgt gaaggagtga aagatttcca cttctatacg cttaaccgcg
     7141 ctgaaatgag ttacgcgatt tgccatacgc tgggggttcg acctggttta taaatagtgt
     7201 ggcttttgtg aaaatcacac agtgatcaca aattttaaac agagcacaaa atgctgcctc
     7261 gaaatgaggg cgggaaaata aggttatcag ccttgttttc tccctcatta cttgaaggat
     7321 atgaacctaa aacccttttt tataaagcat ttgtccgaat tcggacataa tcaaaaaagc
     7381 ttaattacga tcaatttgat ctacatctct ttaaccaaca atatgtaaga tctcaactat
     7441 cgcatccgtg gattaattca attataactt ctctctaacg ctgtgtatcg taacggtaac
     7501 actgtagagg ggagcacatt gatgagcacg tcagacgata tccataacac gacagccact
     7561 ggcaaatgcc cgttccatca gggcggtcac gaccagagcg cgggggcggg cacaaccact
     7621 cgcgactggt ggccaaatca actccgtgtt gacctgttaa accaacattc taatcgttct
     7681 aacccgctgg gtgaggactt tgactaccgc aaagaattca gcaaattaga ttactacggc
     7741 ctgaaaaaag atctgaaagc cctgctgaca gaatctcagc cgtggtggcc tgccgactgg
     7801 ggcagctacg ccggtctgtt tattcgtatg gcctggcacg gcgcggggac ttaccgttca
     7861 atcgatggac gcggtggcgc gggtcgtggt cagcaacgtt ttgcaccgct gaactcctgg
     7921 ccggataacg taagcctcga taaagcgcgt cgcctgttgt ggccaatcaa acagaaatat
     7981 ggtcagaaaa tctcctgggc cgacctgttt atcctcgcgg gtaatgtggc gctggaaaac
     8041 tccggcttcc gtaccttcgg ttttggtgcc ggtcgtgaag acgtctggga accggatctg
     8101 gatgttaact ggggtgatga aaaagcctgg ctgactcacc gtcatccgga agcgctggcg
     8161 aaagcaccgc tgggtgcaac cgagatgggt ctgatttacg ttaacccgga aggcccggat
     8221 cacagcggcg aaccgctttc tgcggcagca gctatccgcg cgaccttcgg caacatgggc
     8281 atgaacgacg aagaaaccgt ggcgctgatt gcgggtggtc atacgctggg taaaactcac
     8341 ggtgccggtc cgacatcaaa tgtaggtcct gatccagaag ctgcaccgat tgaagaacaa
     8401 ggtttaggct gggcgagcac ttacggcagc ggcgttggcg cagatgccat tacctctggt
     8461 ctggaagttg tctggaccca gacgccgacc cagtggagca actatttctt cgagaacctg
     8521 ttcaaatatg agtgggtaca gacccgcagt ccggctggcg caatccagtt cgaagcggta
     8581 gacgcaccgg aaattatccc ggatccgttc gatccgtcga agaaacgtaa accgacaatg
     8641 ctggtgaccg acctgacgct gcgttttgat cctgagttcg agaagatctc tcgtcgtttc
     8701 ctcaacgatc cgcaggcgtt caacgaagcc tttgcccgtg cctggttcaa actgacgcac
     8761 agggatatgg ggccgaaatc tcgctacatc gggccggaag tgccgaaaga agatctgatc
     8821 tggcaagatc cgctgccgca gccgatctac aacccgaccg agcaggacat tatcgatctg
     8881 aaattcgcga ttgcggattc tggtctgtct gttagtgagc tggtatcggt ggcctgggct
     8941 tctgcttcta ccttccgtgg tggcgacaaa cgcggtggtg ccaacggtgc gcgtctggca
     9001 ttaatgccgc agcgcgactg ggatgtgaac gccgcagccg ttcgtgctct gcctgttctg
     9061 gagaaaatcc agaaagagtc tggtaaagcc tcgctggcgg atatcatcgt gctggctggt
     9121 gtggttggtg ttgagaaagc cgcaagcgcc gcaggtttga gcattcatgt accgtttgcg
     9181 ccgggccgcg ttgatgcgcg tcaggatcag actgacattg agatgttcga gctgctggag
     9241 ccaattgctg acggtttccg taactatcgc gctcgtctgg atgtttccac caccgagtca
     9301 ctgctgatcg acaaagccca gcaactgacc ctgaccgcgc cggaaatgac cgcgctggtg
     9361 ggcgggatgc gcgtactggg tgccaacttc gatggcagca aaaacggcgt cttcactgac
     9421 cgcgttggcg tattgagcaa tgacttcttc gtgaacttgc tggatatgcg ttacgagtgg
     9481 aaagcgaccg acgaatcgaa agagctgttt gaaggccgtg accgcgaaac cggcgaagtg
     9541 aaatacactg ccagccgtgc ggatctggta tttggttcta actctgtcct gcgtgcggtg
     9601 gctgaagttt acgccagcag cgatgcccac gagaagtttg ttaaagactt cgtggcggca
     9661 tgggtgaaag tgatgaacct cgaccgtttc gacctgctgt aatctgacct tcttcagcga
     9721 ctgcctttca ggcagtcgct gaagtttctt taccggcgta tagtgtccac aggaaaacta
     9781 cacactggat ctctcatgtc tgccgcagga aagagcaacc cactggcaat cagtggcctg
     9841 gttgtgctca cacttatctg gagttatagc tggattttca tgaagcaagt caccagctac
     9901 atcggtgcct tcgactttac cgccttacgc tgcattttcg gcgctctcgt tttattcatc
     9961 gtccttttat tacgtggtcg cggaatgcgc ccgacaccgt ttaaatacac cttagccatt
    10021 gccctgttac aaacctgcgg gatggttggc ctggcgcagt gggcgctggt cagcggcgga
    10081 gcggggaagg tggcgatcct gagctatacc atgccgttct gggtggtgat tttcgccgcg
    10141 gtgtttctcg gtgaacgcct gcgacgtggg caatatttcg cgattctgat tgccgctttc
    10201 ggcttatttt tggtgttgca gccgtggcaa ctcgatttct cttcgatgaa aagtgccatg
    10261 ctggcaatcc tctccggtgt tagttggggg gcgagcgcga ttgttgctaa acgtctgtat
    10321 gcccgtcatc cgcgcgtgga tttattgtcg ttaacatcct ggcagatgct ctatgcggcg
    10381 ctggtgatga gtgtggtcgc tttactggtg ccgcaacgtg aaattgactg gcagcccacc
    10441 gtgttctggg cgctggccta cagtgcgatt ctggcgacgg cactggcgtg gagcttatgg
    10501 ttgtttgtat tgaaaaactt gcctgccagt attgccagct taagcacact ggccgttccc
    10561 gtttgcggcg tactcttttc ctggtggctg ctcggcgaga atccgggggc cgttgaaggt
    10621 agcggtattg tgctgattgt gatggcactg gcgctggtga gccgtaagaa aaaagaagcc
    10681 gttagtgtaa aaaggatctg aattttttct tcatgtgggg cgatcgctta tttaacaaaa
    10741 taacgataat gccccaccat gcgccagcta aacagcacat cttcttcctg cgcacctgcg
    10801 ccaatgttat gtatcaccag cggcgtaccg tcgcgggcga agccatctga caccacccca
    10861 atatgtgcca gcccgttatc taatcgccag gagacaatat cgcccgcttg atagtcactg
    10921 gggttcttgc tggtggggcg tgttttatcg tggcgggtaa accaggtttc cagattaggc
    10981 acccgacggt gatcgatgtt gctgtccggg cgctttaact tccatttttg cgggtactca
    11041 gcaaaattct tcgccatatc ttcgtgaacc agtttctgca aatcgacctt ctggctgcgc
    11101 aatgcgcgga tcaccacatc ggaacataca ccgcgttctt gcggaacatc accgccagga
    11161 taggtaagct gcatatacgc cggatcgtaa aatagcgtgc tgccgatttg ctgtctggca
    11221 ccgtctgcga tggcaaggtt ggtattggcc tggatttgta ccacggttgg tggaacggcg
    11281 ggagatttta aggagtggct ggtaaatgcc gttagcaggc tgaccagcac cagtgaagct
    11341 ttcatctgtt tcgtccgtga aaataagagt cacagattcg accttccggg ataacgattg
    11401 ttctgtagag aaaatgtcca gaagcggata aaaaattcct gaacaacgtg ggagtttcag
    11461 attactggca cagagtgccg gatgcggagt aaacgcctta tccggcctac aaaagcacgc
    11521 aaattcaata tattgcagag attgcgtagg cctgataagc gtagcgcatc aggcaatttt
    11581 gcgttcaaac tcccggacaa gccgggagtt tggagtaggt tattcccact cttgcaggaa
    11641 acgctgaccg tactggtcgg ctaccagcag agcggcgtaa acctgatctg gcgtcgcgcc
    11701 gccaggcatg ttgtggatgg tttcaccttc tgcacatgcc gcttctgcca caattcgcat
    11761 tttcgccggg acatcttctt taatatccag ttgagcgaga gttattggca aacctaccgc
    11821 atggctaagt gcagctacgg tttcgatttc ctcaaccggt gcgttttcca gaaccagctg
    11881 cgtcagcgta ccaaatgcca ctttttcacc gtgataatag tgatgcgcgt ccgggatagc
    11941 ggtcaggccg ttatgcactg cgtgcgccgc agccagacca ccactttcaa agcctacacc
    12001 gctcaaatag gtgttcgctt caatcacgcg ctccagcgcc ggagtcacta catgctgttc
    12061 ggcggcaagc atcgcttttt cgccttcttc cagcagagtg ttgtagcaca gttctgccag
    12121 tgccagcgcc gcctgggtac acttgccgcc cgccatagtg gtagcgccgc tacgggaaca
    12181 ggcgcgtgct tcaaaccagg ttgccagcgc atcgccgata cccgccgcta acagacgtgc
    12241 aggtgcgcca gcgacgattt tggtgtcgac aatgaccata ttcgggttat ttggcaacag
    12301 cagatagcgg tcaaactcac cctcatcggt gtagataaca gacaatgcgc tgcacggtgc
    12361 gtcggtagag gcgatagtcg gtgcgatcgc taccgcaaca cccatgaaat gtgccagtgc
    12421 tttggcagta tcgagagttt ttccgccacc gataccgaga attgcgccac actgcgcagt
    12481 ctccgcgatg ccacgcagac ggtcgatctc attttgcgaa cattcaccgc caaacggcgc
    12541 aatttctact accagtccag catctttaaa gcttttctcg acagtggatt gagcaaaacc
    12601 taaaacaaat ttgtcaccca ccactaacca gcgttctgcc agcggcttca ggtattcgcc
    12661 cagacgatta atcacatcag cgccctggat gtatttaccc ggtgattgaa taatgcggtc
    12721 cataattgct cctttagaga tgagtagtgc caaatgcggc attccagtcg tgttcgaact
    12781 tctctatagc tgactctacc gcaggggtgt tgagcatttg ttgcgctaca tctaagggca
    12841 gggtgatgga ttcacatcct gccagtaaac agtccagcgc ctgacgcggc gttttaaagc
    12901 tggctgccag caccatgctt tctggcgcgt gcatttctaa cagcgcttgc agctcctgaa
    12961 ccgtacgaat gccgtctccg ccctgggcat ctacgcggtt aacatacgga gcaacgtatt
    13021 ttgcccctgc cagcgcggcc agtaaccctt gtgcagcact gtacactgcg gtgccaagcg
    13081 tagtaatacc ctcttttttc agcatcttaa tggcggccag accttcggaa gtcaccggga
    13141 ttttcaccac aatgcccgga atggcgtcgc gcaggcgctt cgcttcttcc accataccct
    13201 gcgcgtcacg actcatagtc tgagcaaaca gaatgccctc atcaccaatc gctttttgca
    13261 gacgcggcag cacttcccag atggactcct tgctggcagc gataatgctc gggttagttg
    13321 tcaccccggc aatcgggaat atgcgtgcca gacgttcgac ttctgcgacg ttagcggtgt
    13381 ccagatacag ttccatgatg tttcctttta cagttccagt tcatgttgca gcaggctggc
    13441 gatagcgtcg gcagaggcgg cgttaaccag cgcgttacgg aattcttcgt gcatgatgcg
    13501 gcgagcgagg cgcgagaaaa tgcgcatgtg ctgatcgccc gcagcgtgtt tgttcagggt
    13561 taacatgatg atgaattgcg cttcatcatc gccccagcgc accggcgctt gcagacgcgc
    13621 tacgctgatg gtggactgct caatgtgttc tgatttgctg tggggaatgg caaaactaaa
    13681 gcccagaccg gtagagaaaa cggcctcgcg cgcccacaag tcggcttcca gtttacgcgg
    13741 atagcggcag cggcccgcca gcagcaggtt atcggtcatg cctttgagca cttcttcttt
    13801 gctgcgccag tcgctttcca gcgtgatgca ctcggcggtg accagcggtg cgtcctgttg
    13861 ggtcatgcgg aattgcgcca gcaggtgttc cacttccagc gaagtacggc aggccattgc
    13921 ctggttgagc aactggcggc actcacggct atcaagttgc gccattcgcg ctttcgccgc
    13981 cggaattgac ggtgcgctca tgctcagctc atccagccct aagccgatca gcaacggcag
    14041 cacggaacct ttcgctccca gttcaccgca cagaccaatc catttgccct ggcgatgcac
    14101 cgcctgcacg gcgtaatcga gcgcccgcaa gaatgccgga ttcaggctgt tgtagtgacg
    14161 agtaacctta gcgttatcgc gatccactgc cagcagatat tgcgtcaggt cattactacc
    14221 gatactaaag aaatcaatct cttcgcagca ttgatcgatg atgaacatca ccgacggcac
    14281 ttccagcata atgccgagct ggatcttctc atcaaacgga atgtgttcgt tacgcagttg
    14341 ctgtttggct tccgtcagtt tttctttcac ccataagatc tcttccattg aagagatcat
    14401 cgggatcatg attttcaggc tgccgtgagc ggaggcgcgc aggatcgacc gtagttgtgt
    14461 ggtaaataac gacgcgtact cttcataaat acgcacagcg cgataaccga ggaacgggtt
    14521 agcttctgcc ggaatgttca gataatcaac gggtttgtcg ccgccaatgt ccatagtgcg
    14581 cacaataatg ctgcgtccgt tggcggactc cagcgcctga caaaaaatgt tgtacagctc
    14641 gctttcgccc ggtgcactgg tgcgatccat atagagcatt tcagtgcgga acaaaccaac
    14701 gccttccgca ccattgccga atgcggcctg cgcttccacg gagtgagcga tgttagcggc
    14761 aatttcaatg cggataccgt cagcggtacg ggcttgttgg gtcagccaga cacgctgttg
    14821 ctcacgaagg gcgtcctgta cgcgggcttc ttgctgataa taacgagcta cggcttcccc
    14881 tggctcaacc acaatcgccc cggcgttgcc gtcgatataa atcgtttgat gctgccacgg
    14941 agtaagggca tcaatatcta caccaaccag tgtcggaatg ttgaacgaac gggcaagaat
    15001 tacggtatgt gaggtggtgc cgccactttt cagtaacaga cctttgaggt gatttttatc
    15061 cagttcgagg aactggctgg gggtcagttc atcagccata caaatggcgg gctgcgtcag
    15121 tttgcccggt gccgggaagc gttgctcacc gtagatttgc tggagtaact ggaagcagac
    15181 gtcgcgtacg tccagggcgc gttcttgcag gtatctgctg ctggaacggg cgaactcttc
    15241 gcaaaagtga ttcgcactgg cgacaatcgc ttcggcacag cttaatccct cgctgacgcc
    15301 tgccagtaag tgctgacgta aagaagtatc gccagccagg gatcggtgag cttccagtat
    15361 cgcgctggta gcaccgtcgc tatccagcag acgaaactct atgtttttca gtaccagcgt
    15421 caggccgttt tccagtgcgg attgctcggc gtcaacgtct ttggctgcgg gaagattact
    15481 cagcgcattg agatctaaag aagagatcgg cgtcagaatg ccgcctgcgc taccgctgca
    15541 cacggtgcgg gcgcggataa tttgcggatt cagattggtc agtgaaaccg gcagtggttc
    15601 cagttcgtca gatttaactt ccgccagcgg cgcatcgcag tgggggaatt catcgcgcag
    15661 ccattggctt aaacactggt gcgcttcctg ttcgtcggcc ccggaaatga gtaactggca
    15721 gttatcgccc gccagcgtgt cggtgccaat cagcgcaagg gcgcttttgg cgttgccctt
    15781 gcggtcagtg cgcaggttat gccactcaat ttgtgatgaa aaagtattac acagcgtttc
    15841 aacgtggctt gccggacgcg catgtacgcc gttaggtagc tcacaaataa attccacaat
    15901 cagggccatt gcctctctcc cggtacggtt ttctgtttac aggataagag cgcacggcaa
    15961 cggcctgcca tgtgacaaat ctgccaaaag ctggacaaat gtaatgtaac cgtcaatttg
    16021 cgacgcgtct cacaagacgc cgttctgcgg catgcttccg ggttatcgca agttatgagg
    16081 cggatcgcat ttttgtactg atattacaaa agtccagtaa atggcctttt tatccactgt
    16141 ttgctctgcc tgcgattgcc tattgtttcc tccatcaaaa atatggacat gggcttttgc
    16201 aagcccggga gcagcttatg aatgagttgg tgcagatcct gaaaaatacc cgtcagcatt
    16261 taatgacggg cgtttcacac atgattccct tcgtggtatc gggcggtatt ttgctggcgg
    16321 tttccgtcat gttgtatggc aaaggcgcag tgccggatgc cgtagccgat ccgaatttga
    16381 aaaaactgtt tgatatcggc gttgcgggtt tgacgctgat ggtgcctttc ctcgccgctt
    16441 acatcggtta ttccattgca gagcgttctg cgctggctcc gtgcgctatc ggtgcctggg
    16501 ttggtaacag ctttggtgcg ggcttctttg gtgcgctgat cgccgggatt atcggcggca
    16561 tcgtggtgca ttacctgaag aaaattccgg tgcataaagt tctgcgctcg gtgatgccta
    16621 tcttcattat tcctatcatc ggcacactga ttaccgcagg catcatgatg tggggcttgg
    16681 gcgagcctgt aggggcgttg accaacagcc tgactcagtg gcttcagggg atgcagcagg
    16741 gcagcattgt tatgctggcg gtgatcatgg gtctgatgct ggcgttcgat atgggcggtc
    16801 cggttaacaa agtggcctat gccttcatgc tgatttgcgt tgctcagggt gtttataccg
    16861 tggtggccat tgctgccgtg ggtatttgta tcccgccgct ggggatgggg ctggcgacgc
    16921 tgattggtcg taaaaatttc tccgcagaag agcgcgaaac cggtaaagcg gcgctggtga
    16981 tggggtgcgt tggggttact gaaggggcga ttcctttcgc cgcagccgat ccgttacgtg
    17041 taattccttc catcatggtt ggttcagttt gtggtgcagt aactgcggcg ctggtcggtg
    17101 cgcagtgcta tgcaggctgg ggtggtctga ttgtgctgcc ggtggttgaa ggtaagttgg
    17161 gttatatcgc agcagtggct gtcggggcgg ttgtgacggc agtctgcgtg aacgtgctga
    17221 aaagtctggc gcgtaaaaat ggatcttcta ctgatgaaaa agaagacgac ctggatttgg
    17281 attttgaaat taattaattg aggaacgagc catgacgaaa attattgcag taaccgcgtg
    17341 tccatctggt gttgcccata cttatatggc ggcagaggcg ctggaaagtg ccgcgaaagc
    17401 aaaaggctgg gaagtgaaag tagaaaccca ggggtcaatt ggtctggaaa acgaactgac
    17461 tgcggaagat gtggcgagcg ccgatatggt gattctgacc aaagatatcg gcatcaagtt
    17521 tgaagagcgt tttgctggca agaccattgt gcgcgtcaac atcagcgacg cggtaaaacg
    17581 cgctgacgcc attatgagca aaattgaagc tcacctggcg caaaccgctt aagttcttct
    17641 cccgctcgca agggcgggtt cgctttccca caggagttcc tcatgacgaa tcgtatctct
    17701 cgcctcaaaa ctgcactgtt tgccaatacc cgtgaaatct cgctggagcg tgcgctgctt
    17761 tataccgcca gccatcggca aaccgaaggc gaaccggtga tattgcgccg ggcgaaagca
    17821 acagcgtata tccttgaaca tgttgaaatt tcgattcgtg atgaagaact gattgccggt
    17881 aaccgcaccg taaaaccacg cgccgggatt atgtcgccgg aaatggaccc ttactggctg
    17941 ctgaaagagc tggaccagtt cccgacgcgt ccgcaggacc gctttgctat cagcgaagaa
    18001 gataaacgta tctaccgcga agagttgttc ccgtactggg aaaaacgttc gatgaaagat
    18061 ttcatcaacg ggcagatgac ggatgaagta aaagccgcga ccagcacgca gattttcagc
    18121 atcaaccaga cggataaagg ccaggggcac attattattg attacccgcg cctgctgaat
    18181 cacgggctgg gggagctggt ggcacagatg cagcaacatt gtcagcaaca gccggagaat
    18241 cacttttatc aggcagcgct gttactgctg gaagcctcgc ggaaacacat tttgcgttac
    18301 gccgaactgg cggaaacgat ggcggcaaac tgcacagatg cccagcgtcg cgaagagctg
    18361 ctgactattg cggagatctc ccgccataac gccgaacata agccgcagac gttctggcag
    18421 gcgtgccagt tattctggta catgaacatc attctgcaat acgaatccaa cgccagttcg
    18481 ctgtcgctgg ggcgcttcga ccagtatatg ttgccgttct atcaggcatc tttaacccag
    18541 ggcgaagatc cgacgttcct gaaagaacta ctcgaatctt tatgggtgaa atgcaacgac
    18601 atcgtgctgt tgcgctccac cagtagcgcg cgttatttcg ccggtttccc gaccggctat
    18661 accgcactgc tcggcgggtt aaccgagaac ggacgtagcg cggtgaacgt actttcgttc
    18721 ctctgtctcg acgcctatca aagtgtgcaa ttaccgcaac cgaaccttgg ggtgcgcact
    18781 aacgccttga tcgacacgcc gttcctgatg aaaaccgaag aaaccattcg cctcggcacc
    18841 ggtattccgc aaatctttaa cgatgaagtg gtggtgccag cgttcctcaa ccgtggcgtt
    18901 tcgctggaag atgcgcgcga ctattccgta gtgggctgtg tggaattatc tattcccggc
    18961 agaacctacg gcttgcatga catcgcgatg tttaatctgc tgaaagtgat ggaaatctgc
    19021 ctgcatgaaa atgaaggcaa tgctgcgctg acttatgaag gtttactgga gcagatccgc
    19081 gccaagatca gccactacat caccctgatg gttgagggca gcaatatttg tgatatcggc
    19141 catcgcgact gggcacctgt accgctgctc tcgtctttta tcagcgattg tctggaaaaa
    19201 ggtcgcgata ttaccgatgg cggcgcgcgt tataacttct ccggcgtaca ggggatcggt
    19261 atcgccaacc tgagcgattc tctccatgcg ttgaaaggga tggtttttga tcaacagcgt
    19321 ttaagttttg acgaattgct gtcggtatta aaagccaact ttgcaacgcc agaaggcgaa
    19381 aaagtccgcg ctcgcttaat taaccgcttt gagaaatacg gtaacgatat cgacgaggtg
    19441 gataacatta gcgccgaact gttgcgccac tactgcaaag aagtggaaaa ataccagaac
    19501 ccgcgcggcg gttacttcac gccgggatcg tataccgttt ctgctcacgt cccgttggga
    19561 tcggtggttg gcgcgacgcc agacggtcgt tttgccggag aacagctggc agacggcggc
    19621 ttgtcaccca tgctgggcca ggatgcacaa gggccaacag cggtactgaa gtcagtcagt
    19681 aagctcgata acacgctgct gtctaacggt acgttgctga acgtgaaatt cactccggcg
    19741 accctggaag gtgaagcggg attacgcaaa ctggccgact tcttacgggc gtttacccag
    19801 cttaagttgc agcatattca gtttaacgtg gtgaacgccg acacgttgcg ggaagcgcaa
    19861 cagcgcccac aagattatgc cgggctggtg gtgcgcgttg ccggatatag cgccttcttt
    19921 atcgaactgt cgaaggagat ccaggatgac atcatccgcc ggacagcgca tcagctgtaa
    19981 cgttgtggaa acgcgccgca atgatgcggc gcgcattttc aacattcagc gttattcact
    20041 gaatgacggc gagggcattc gtacggtggt cttttttaaa ggctgtccgc atctttgccc
    20101 gtggtgtgct aatccggagt cgatctccgg caaaatccag acggtacgca gagaggcgaa
    20161 atgtctgcac tgtgcgaaat gtttgcgtga tgcggatgaa tgcccctccg gggcgtttga
    20221 acggattggt cgcgatatca gccttgacgc gctggaacgg gaagtgatga aagatgacat
    20281 tttctttcgc acgtccggcg gcggcgtcac gctttctggc ggcgaagtgt taatgcaggc
    20341 ggagtttgct acccgttttt tacagcggct gcggttgtgg ggcgtgtcat gcgccattga
    20401 aactgccgga gacgcgccag ccagcaagct gttaccgctg gcgaaattgt gcgatgaagt
    20461 gttgttcgat ttaaaaatta tggacgcgac tcaggcgcgg gatgtggtga agatgaacct
    20521 gccacgcgtg ctggagaatc tgcgtttgct ggtgagtgag ggcgtcaacg tgatcccgcg
    20581 tttaccgctg atccctggtt tcacgctcag ccgggagaat atgcagcagg cgctggacgt
    20641 gctgatcccg ctgaatatca ggcagatcca tctgttaccg tttcatcagt acggcgaacc
    20701 gaaataccgc ttgctgggga aaacatggtc gatgaaagag gtgcccgcgc cgtcgtcagc
    20761 tgatgtggca acgatgcgcg aaatggcaga acgggccgga tttcaggtta ccgtgggagg
    20821 ttaaaatggc atacctggtg gcagtaaccg cctgcgtaag cggcgtggcg catacttata
    20881 tggcggcgga acggctggaa aagttgtgcc tgttagagaa gtggggcatc agcattgaaa
    20941 ctcagggcgc gctgggaacg gagaatcgtt tagcggacga ggatatccgc cgggcggatg
    21001 ttgcgctgtt aattacggat atcgagcttg ccggtgccga gcgatttgaa cattgccgct
    21061 atgtgcaatg cagcatctac gcattcctgc gtgagccgca gcgggtaatg agcgcggtgc
    21121 gcaaagtgct ttctgcgccg cagcaaaccc atcttattct ggagtagtcg gtttttctgt
    21181 cagttggctg tggtactgcc gacgatattc cgacggcgag cgttcggtgt ttttacgaaa
    21241 cagacggcag aagtagttgc tgtcgacaaa gccgcaggcg tgcgccactt cttttacctt
    21301 caggtcatag cctttcaata acgtcttagc gtgctccagt cgcgtgtgat tcaggtattc
    21361 gttaaaacca atggcccccg ttttttgaaa caggtgcgag agatagtttg gtgaaatata
    21421 gaatgcctgt gcgacagatt cgcgggtaag cgcggaggca tagcgttcgt cgatataatc
    21481 gcgaatagct tcgaatagtg cctggctgcg tgaggcggtc tggatttggc tgccaagcaa
    21541 atcgcggcag tggctaagca ggctggcgac gataagcctt gccgtttgct gctcctgcgg
    21601 ctgcatttgc atttcattga gcgtttgtag cagaaaagaa cctatgcgtg ggccgcgtcg
    21661 ggcgacgtgt tgcttcgcca gattttgata ttgtttgcca tcccattgca cgacgctgaa
    21721 gccgagttgc tgtttgccaa acagcacgct aaaggtagta gcgggggctt gccattgtgg
    21781 aaaattccag ccaccagcgg gaacgtacag cacatcgccg ggaactaacg ttgctccagc
    21841 gccggtatca acaaactcac cttccagcac aatttccagc cgtggaaaat cgacctgata
    21901 cgcaagatcg ggcaccgggc cgttactgct ggcaaagtaa atctggcgca gggacagcgg
    21961 gccgttgatc aggcgggaga gcagatagct gacgtcgtga tacatgttat tcccttaggt
    22021 atgtagacgg gaaaacagta actggcatta ttaaaaaagg ccacctgaaa agtgtggcct
    22081 gaggggagtt cgatgtaaac attgcggcag ttgtattgcc ggatgtggcg taaacgcctt
    22141 atccagtcta ccgccgcgat ctgtaggccg gataagacgc gtcaagcgtc gcatccggca
    22201 aatagcgcct ggctgataat tactgattac ccacctggtc gccatacggc agcgtgtcgt
    22261 aatcgatcag cgcgtttttc ttgtacgggt taccaatcca gcgggtagtt tctttgaact
    22321 gcggattcac cactgaacgg gttggatcgt aaccgtcgta agataagcct gctaaatctg
    22381 accaggtgtg gatcagttcc gcaaggctgt atttacgatc aacatcctgc gagaaatcac
    22441 gcggatgagt cgcttgccat ttttctgacg tccacagcag gaacggaatg gtgtacatat
    22501 gacgcgtcgg gttgtcctca ttacgcccct gggttttatg aggcggcgtg tcgtaaacct
    22561 cttcaccgtg gtcagagaag taaaccagga aaccgttcgg gtctgctgct ttaaagtctt
    22621 taatcaggct ggcaaccaca tgatcgttat acaggttagc gttgtcataa tcgttatatg
    22681 actccagctc ttccgcgttt aatcccggcg gaacatgatc ggtattgcca tcaaacttgc
    22741 cctgattttc cgggtagcgg tatttgtatt tgatatgcgt ccccagcaga tgaacgatga
    22801 tcagtttctt cggcgcaggg tcattcagca cttcctggaa cggcttcagc acgttggtgt
    22861 cgtattcacg cgcactctgc gtacgttgct ggttcatgta gtactgcttg tcggtctggc
    22921 gcgaaaatac cgtcagcatg gtattgcggg cggtcatcgt ctgctggttg gtgatccaga
    22981 aggttttata acccgcctgt ttcatcatgt tcatcagcga cggttgcgtc agatacagat
    23041 ccgggttctt ttcattggca aaggtcagcg cctgttgcag gatttcaatg gtgtacggac
    23101 gagacgtaac tacgttatta aacacggtta gattcggatc ggttttatgc agcgcatcca
    23161 gctccggcgt ggtttcacgc ggataaccgt acagactcat gcgtccacgc tgggttgact
    23221 cgccaatcac cagcactaag gtgcgcggtt cgttacccga ttcatctttg aaattagcca
    23281 gcggcggcaa ggcattattt tcattcagta gctttgttag cgagtttagt tgctgacgat
    23341 actgataata gccggtcagg aattgccacg gtgcggcagg ctccatacgc gaggccaggt
    23401 tatccaacgt tttctcaaac ggcttgtttt tgataaacgt attcatggcg atcggatgca
    23461 gaatcaagcc ataaagcagg gcaaaagaga caacataacg ccacggcttt ggaatataga
    23521 ccgggcgcag gcgtgtccac agcagaactg ccaccgccgt ataggccagc gcgataagca
    23581 caattttcag gctgaaatac tggcttaaat actcgctggc ttcgttggtg ttggtttcga
    23641 acatcacaaa cagaacgctc tgcgagaact cctgaccgta gatgacgtag tagcacagcg
    23701 ccgccagaga ggccgcccat agcaccacgc cgatcactgc ggcaataatt ttgatccgct
    23761 tcggaaagag gaataccgga attaaccaca gcgagctgaa taacagcgag tcgcgaatgc
    23821 cgttggtgcc actataacca ctgatgtaaa taatggcctg tagcagagta gagaaaaacc
    23881 aaaagtagag cagtgcccaa cccagggctt tccagctaaa aagaggttta gcctggactt
    23941 ctgtggaatg catagtaaga acctgtcttg aaaaaatatc gccgaatgta acgacaattc
    24001 cttaaggata tctgaaggga tatccagaat ttgaataaaa tgcagacaga aatatattga
    24061 aaacgagggt gttagaacag aagtatttca gaaaaccctc gcgcaaaagc acgagggttt
    24121 gcaggagagg aagattagcc ggtattacgc atacctgccg caatcccggc aatagtgacc
    24181 attaacgcct gctcgacgcg aggatccggt tcctggcctt ctttttctgc ctggcgggag
    24241 cggtgcagca actcggcttg caatacgttc agcgggtcgg tgtaaatatt ccgtagctga
    24301 atagactctg caatccacgg cagatcggcc atcagatggg aatcgttggc aatcgccagc
    24361 accactttga tgtcttcttc ttgcagattg cgtaactctt tacctaacgg ccacagtgct
    24421 ttgtctacca ggcgttggtc atagtattcc gccagccaca ggtctgcttt ggcgaagacc
    24481 atctccagca tgccgagacg cgtcgagaag aatggccaat cgcggcacat agcctccagc
    24541 tcgctctgtt tgccatcttc gaccactttt tgcagcgccg tacctgcacc cagccaggcg
    24601 gggagcatca ggcggttttg cgtccaggcg aaaatccacg gaatggcgcg cagtgactcg
    24661 acgccgccgg tcgggcgacg tttcgccgga cgtgaaccca acggcagttt gcccagttct
    24721 tgttccggcg tagcggagcg gaagtaaggc acaaaatctt tgttttcacg tacgtagcca
    24781 cggtagagat cgcaggagat gactgatagt tcatccataa tgcgacgcca gctctctttc
    24841 ggctcaggtg gtggcagcag gttagcttcc agaatcgccc cggtgtacag cgacaggctg
    24901 ctgacggtga tttctggcag accatattta aagcggatca tctcgccctg ttcagtcacg
    24961 cgcaggccac ctttcaggct tcctggcggt tgtgacagca gcgccgcatg agcaggtgcg
    25021 ccgccgcgac caatggaacc gccgcgaccg tggaacaacg tcagctcaat acctgctttt
    25081 tcgcaggttt tgattaatgc atcctgtgcc tgatattgcg cccaggacgc tgccatcacg
    25141 cccgcatctt ttgctgagtc ggaatagcca atcatcacca tctgtttgcc ctgaatcagg
    25201 ccgcgatacc agtcgatatt gagcagctgg gtcatgacat cgttggcgtt gttcaggtca
    25261 tcgagggttt caaacagcgg agcaaccggc atcgcaaacc cgatacccgc ttctttcagc
    25321 agcaggtgga cagccagcac gtcggacggc gttttcgcca tcgagatcac gtaggcggca
    25381 atggagcctt gcggtgcttc ggcaatcacc tggcaggtat cgagcacttc gcgcgtttcg
    25441 gcgcttggtt gccagttgcg cggcagaagc ggacgtttgg agttcagttc gcggatcagg
    25501 aacgcctgtt tgtcggcctc tgaccagctt tcgtagtcgc cgataccgag gtagcgggtc
    25561 agctcgccca gcgcttcggt atggcgggtg ctctcctgac ggatatcaat acggaccagc
    25621 ggtacgccga aacatttcac gcggcgcagg gtgtcgagca gatcgccgtt ggcgataata
    25681 cccatgccac acgcctgaag tgactggtag caagcgtaga gcggttccca cagttcttcg
    25741 ttttgtgtca gcaggccttc tggttttggc agttcttcgc ctttcaggcg cgcttccagc
    25801 catgcctgtg tcgccatcag gcgagaacgc aggtttttca tcagatagcg atacggttct
    25861 gcggcacctt cttcgccaac cagcgccagc agttcagggg tcgcttcaac catcgacagt
    25921 tcagaaacca gaacctgaat atctttcagg aacagatcgg tggctttcca gcggctgagc
    25981 agcaggacgt ggcgggtgat atcggcagtg acgttcgggt tgccgtcgcg gtcaccgccc
    26041 atccacgagg taaaacgtac cggaacaaat tcgacgggca gtttgtagcc gaggttctct
    26101 tccagttgtt cgttcagttc gcgcaggtaa ttcggtacgc cttgccacag gctgttttct
    26161 actacggcaa agccccattt ggcttcatct accgggcttg gacgcagctt acggatttca
    26221 tcggtatgcc atgactgggc gatcaactgg cgcaggcggc gcattagctg gttgtgttcg
    26281 tagtcagcga tatctttgtt atcgagctgt tttaaacagg cgttcacttc caccattttg
    26341 tggatcagtg tacgacgggt aatttcggtt gggtgagccg tgaggaccag ttccagcgac
    26401 agcgattcca ctgctttttt gatggtgtct tcgctcagtt ccggctggtt tttcagtttg
    26461 cgcagggtgc gggcgatcac ttccgggttg ctggcagctt cgcctttcgg cgaaatgctg
    26521 tggtattgct cggcggtgtt ggccaggttc aggaactgac taaacgcacg cgcaacgggc
    26581 agcagctcgt cgttcgacaa attttgtaag gtggtgagta actcctggcg gttagcatca
    26641 ttgccagcgc gtgaagattt ggacaactta cggatagttt ctacgcgttc aagaatgtgt
    26701 tctcccaacg catccttgat ggtttctccc agcactttgc cgagcatact gacattacta
    26761 cgcaatgcgg aatattgttc gttcatatta ccccagacac cccatcttat cgtttgatag
    26821 ccctgtatcc ttcacgtcgc attggcgcga atatgctcgg gctttgcttt tcgtcgtctt
    26881 ttataaagcc acgtaaaagc ggtgacgtca aatgctgcga aatcgcttca gcaaacgaat
    26941 aaatagcagg aatttacgtc attaaattca cgacgcttta aataagcgta acttatggga
    27001 atgttaaaaa atcgcctcaa gtaacaccaa aggtgtaggt cggataagat gcgcaagcat
    27061 cgcatccgac attattgcgg cactggagtt tggcaacagt gccggatgcg gcgcaagcgc
    27121 cttattcggc ctacggttgg gcatcgtttg agtcactgtc ggtcggataa gatgcgcaag
    27181 tatcgcattc gacattattg cggcactgga gtttggcaac agtgccggat gcggcgcgag
    27241 cgccttatcc ggcctacggt tgggcatcgt ttgagtcact gtaggtcgga taagatgcgc
    27301 aagcatcgca tccgacatta ttgcggcact ggagtttgac aacagtgccg gatgcggcgc
    27361 gagcgcctta tccggcctac gttttaatgc cagcaaaaat ggtgaattat ctgggttatc
    27421 agttcgcggg tgggcttgat aaaccgtgtt tccagaaatt catcaggttg atgagcctga
    27481 ttaattgagc caggccccaa caccagcgtc gggcataacg tttgaataaa cggcgcttcg
    27541 gtacagtagt tcaccacttc ggtctttgct ccgagcaatt tctcaactac ttcaaccagt
    27601 tgatgattcg gtgggcattc atagccaggg atcggcggat gcagctcgtc gaccgtcaga
    27661 cgacccggcc agcgttcgct caccggagcc aatgcatcgt tgagcaaacc attaagttca
    27721 ttgagtgtca tgccaggcag cggacgaata tccatatgca actcacagca agcgcaaata
    27781 cggttagaag cgtcgccacc gtgaatatgc ccgaggttga gcgtagggta tggcacggta
    27841 aacgcgtcgt agtgataacg ttctttcagg ttatcgcgca attgcaaaat atgcccgata
    27901 gcgtcgtgca ttagttcgat agcgttaact ccgcgtgctg gatcgctgga gtgccccgac
    27961 tggccctgaa tacggatggc gttagagata tgacctttat gtgcgcgtac cggttgtagt
    28021 gacgtcggtt cgccaatgat ggcgcaatcc gggcgcaggg cggtagtttc ggcaaaataa
    28081 cgcgctccgg ccatactggt ttcttcatca gcagtcgcca gaatgtagag cggttttttc
    28141 agtttcgtga cgtcgacatc gcgtagcgta tcaaggataa acgcaaaaaa gcctttcatg
    28201 tcggcggtgc ctaagccgta aagcttgccg tcatgctccg tcagtgtaaa cggatcgcgc
    28261 gtccagcgac cgtcatcaaa tggcaccgta tcggtatgcc ccgccagcaa caagccgcca
    28321 gccccctgtc cgatacttgc cagcatattg aatttgttgc gagttcctgg cacaggctgc
    28381 acttccacat tgaagcctaa atctttaaac cagtccgcca gcagagtgat taaatctgca
    28441 ttgctttgat cgagtgcctc ttccgtggcg cttattgaag gtgtggcaat cagagcacgg
    28501 taaatctcga taaatggcgg taatttgttt ttcattgttg acacacctct ggtcatgata
    28561 gtatcaatat tcatgcagta tttatgaata aaaatacact aacgttgagc gtaataaaac
    28621 ccaccagccg taaggtgaat gttttacgtt taacctggca accagacata agaaggtgaa
    28681 tagccccgat gttgaatacg ctgattgtgg gtgccagcgg ctacgctggc gcagagctag
    28741 tgacctatgt aaatcgccat ccgcatatga acataaccgc tttgactgtt tcagcgcaaa
    28801 gcaatgatgc gggaaagtta atctccgatt tgcatccgca gctaaaaggc atcgttgagc
    28861 tgccgttgca gccgatgtcg gatatcagcg agtttagccc aggggtggac gtagtgtttc
    28921 tcgccaccgc ccatgaagtt agccacgatt tagcgccgca gtttcttgaa gcgggctgcg
    28981 tggtgttcga cctttccggc gcgtttcgtg ttaacgacgc caccttctat gaaaaatatt
    29041 acggctttac ccatcaatac ccggaactgc tggaacaggc agcctacggt ctggcggagt
    29101 ggtgcggtaa taaattaaaa gaagcgaatt tgattgcggt gccaggctgt tatccgacgg
    29161 cggcacagct ggcgctgaaa ccgttgattg atgccgatct tcttgacctc aatcagtggc
    29221 cggtgatcaa cgccaccagc ggcgtgagcg gtgcaggacg taaggcagcg atttcaaata
    29281 gtttttgtga agttagcctg caaccgtatg gcgtctttac tcatcgccat caaccagaga
    29341 tcgccacaca cctcggtgct gacgttatct tcaccccaca tctgggcaat ttcccgcgcg
    29401 gcattctcga aaccattacc tgccgcctga aatcgggtgt gacccaggcg caagtcgcgc
    29461 aagtgttaca gcaggcgtat gcccataaac cgctggtgcg gctgtatgac aaaggcgttc
    29521 cggcgctgaa aaatgtcgtt gggctgccat tttgcgatat cgggtttgcc gttcagggcg
    29581 agcatttgat tattgtggcg accgaagaca acttactgaa aggcgcggcg gcacaagcgg
    29641 tacagtgcgc caatattcgt ttcggctatg cggaaacgca gtctcttatt taagggtgca
    29701 atgatgaatc cattaattat caaactgggt ggcgtactgc tggatagtga agaggcgctg
    29761 gagcgtctgt ttagcgcact ggtgaattat cgtgagtcac atcagcgtcc gctggtgatt
    29821 gtccacggcg gcggttgcgt ggtggatgag ctgatgaaag ggctgaatct gccggtgaaa
    29881 aagaaaaatg gcctgcgggt gacgcctgct gatcagatag acattatcac cggagcactg
    29941 gcgggaacgg caaataaaac cctgttggca tgggcgaaga aacatcagat tgcggccgta
    30001 ggtttgtttc tcggtgacgg cgacagcgtc aaagtgaccc ggcttgatga agagttaggt
    30061 catgttggac tggcgcagcc aggctcgcct aagcttatca actccttgct ggagaacggt
    30121 tatctgccgg tggtcagctc cattggcgta acagacgaag ggcaactgat gaacgtcaat
    30181 gccgaccagg cggcaacggc gctggcggca acgctgggcg cggatctgat tttgctctcc
    30241 gacgtcagcg gcattctcga cggcaaaggg caacgcattg ccgaaatgac cgccgcgaaa
    30301 gcagaacaac tgattgagca gggcattatt actgacggca tgatagtgaa agtgaacgcg
    30361 gcgctggatg cggcccgcac gctgggccgt ccggtagata tcgcctcctg gcgtcatgcg
    30421 gagcagcttc cggcactgtt taacggtatg ccgatgggta cgcggatttt agcttaagtt
    30481 ttgttggccg gaggcgcagc tttccggcat tgaatttcaa aataaggaaa cagagttatg
    30541 gcactttggg gcgggcgttt tacccaggca gcagatcaac ggttcaaaca attcaacgac
    30601 tcactgcgct ttgattaccg tctggcggag caggatattg ttggctctgt ggcctggtcc
    30661 aaagccctgg taacggtcgg cgtgttaacc gcagaagagc aggcgcaact ggaagaggcg
    30721 ctgaacgtgc tgctggaaga tgttcgcgcc aggccacaac aaatccttga aagcgacgcc
    30781 gaagatatcc atagctgggt ggaaggcaaa ctgatcgaca aagtgggcca gttaggcaaa
    30841 aagctgcata ccgggcgtag tcgtaatgat caggtagcga ctgacctgaa actgtggtgc
    30901 aaagataccg ttagtgagtt actgacggct aaccggcagc tgcaatcggc gctggtggaa
    30961 accgcacaaa acaatcagga cgcggtcatg ccaggttaca ctcacctgca acgcgcccag
    31021 ccggtgacgt tcgcgcactg gtgcctggcc tatgttgaga tgctggcgcg tgatgaaagc
    31081 cgtttgcagg atgcgcttaa gcgtctggat gtcagcccat taggctgtgg cgcgctggcg
    31141 ggaacggcct atgaaatcga ccgtgaacag ttagcaggct ggctgggctt tgcttcggcg
    31201 acccgtaaca gtctggacag cgtttctgac cgtgaccatg tgctggaact gctgtcggct
    31261 gccgctatcg gcatggtgca tctgtcgcgt tttgctgaag atctgatttt ctttaacacc
    31321 ggcgaagcgg ggtttgtgga gctttctgac cgcgtgactt ccggttcatc attaatgccg
    31381 cagaagaaaa acccggatgc gctggagctg attcgcggta aatgcggtcg ggtgcagggt
    31441 gcgttaaccg gcatgatgat gacgctgaaa ggtttgccgc tggcttacaa caaagatatg
    31501 caggaagaca aagaaggtct gttcgacgcg ctcgatacct ggctggactg cctgcatatg
    31561 gcggcgctgg tgctggacgg cattcaggtg aaacgtccac gttgccagga agcggctcag
    31621 cagggttacg ccaacgccac cgaactggcg gattacctgg tggcgaaagg cgtacctttc
    31681 cgcgaggcgc accatatcgt gggtgaagcg gtggtggaag ccattcgtca gggcaaaccg
    31741 ctggaagatc tgccgctcga cgagttgcag aaattcagcc cggtgattgg cgaagatgtc
    31801 tatccgattt tgtcgctgca atcgtgcctc gacaagcgtg cggcaaaagg cggcgtctca
    31861 ccgcagcagg tggcgcaggc gattgctttt gcaagggcgc ggttggggta agaacattta
    31921 tatgtacaaa tttgagcctg gcttatcgcc gggctttttt atggcaaaaa aaaagcggat
    31981 cctggagatc cgcaaaagtt cacgttggct ttagttattc gagttgagaa actctcgaaa
    32041 cgggcagtga cttcaagggt taaatgaggt gccgctccgt ttctgtgagc aattatcagt
    32101 cagaatgctt gatagggata atcgttcatt gctattctac ctatcgccat gaactatcgt
    32161 ggcgatggag gatggataat gaatattcgt gatcttgagt acctggtggc attggctgaa
    32221 caccgccatt ttcggcgtgc ggcagattcc tgccacgtta gccagccgac gcttagcggg
    32281 caaattcgta agctggaaga tgagctgggc gtgatgttgc tggagcggac cagccgtaaa
    32341 gtgttgttca cccaggcggg aatgctgctg gtggatcagg cgcgtaccgt gctgcgtgag
    32401 gtgaaagtcc ttaaagagat ggcaagccag cagggcgaga cgatgtccgg accgctgcat
    32461 attggtttga ttcccacggt tggaccgtac ctgctaccgc atattatccc gatgctgcac
    32521 cagacctttc caaagctgga aatgtatctg catgaagcac agacccacca gttactggcg
    32581 caactggaca gcggcaaact cgattgcgtg atcctcgcgc tggtgaaaga gagcgaagca
    32641 ttcattgaag tgccgttgtt tgatgagcca atgttgctgg ctatctatga agatcacccg
    32701 tgggcgaacc gcgaatgcgt accgatggcc gatctggcag gggaaaaact gctgatgctg
    32761 gaagatggtc actgtttgcg cgatcaggca atgggtttct gctttgaagc cggggcggat
    32821 gaagatacac acttccgcgc gaccagcctg gagacactgc gtaacatggt ggcggcaggt
    32881 agcgggatca ctttactgcc tgcgctggct gtgccgccgg agcgcaaacg cgatggggtt
    32941 gtttatttgc cgtgtattaa gccggaacct cgccgcacga ttggcctggt ttatcgtcct
    33001 ggctcaccgc tgcgcagccg ctatgagcag ctggcagagg ccatccgcgc aagaatggat
    33061 ggccatttcg ataaagtgtt aaaacaggcg gtttaagcca tttagcgcag ctacccgata
    33121 ggcttccgcc atcgtcgggt agttaaaggt ggtgttgacg aagtactcaa tagtgttgcc
    33181 gccacctttc tgttccataa tcgcctgacc gatatgaata atttcggcag cgcgctcgcc
    33241 aaagcagtga atgcccagaa tctcttttgt ttcccgatgg aacaaaattt tcagcgtgcc
    33301 cacgttcatg ccgacgattt gtgcgcgtgc cagatgtttg aactgggcgc ggcccacttc
    33361 atacggtact ttcatcgcgg tcagctgctg ttcggttttg cccacagagc tgatttccgg
    33421 gatggtgtaa ataccggtag ggatatcttc aatcagatgt gcggtggctt cacctttcac
    33481 cagcgcctgc gcggcaatgc gcccctggtc ataggccgcc gacgccaggc tcggataacc
    33541 aatcacgtcg cccaccgcgt aaacgtgcgg ctgcgcggtc tgatacatgc tgttgacctt
    33601 cagctgtccg cgactgtcag tttccagccc aatgttctgt aacgccagcg aatcggtatt
    33661 accggtacga ccgttggcat agagcaggca gtcagctttc agttttttac ccgacttcag
    33721 atgcatgatc acaccgtcgt cacagccttc gatcttctcg tactcttcgt tgtgacgaat
    33781 cactacgcca ctgttccaga agtgatagga gagagaatct gacatctctt gatcgagaaa
    33841 tgccagcagg cgatcgcggg tgttgatcag atccactttt acatccatac cgcggaagat
    33901 cgacgcatat tcacagccga tcactccagc accatagata agtacatggc gcggttcgtg
    33961 gtgcatgctg agaattgagt cgctgtcgta aatgcgtgga tgggtgaaat caacatctgt
    34021 tggatgatat ggacgagagc cgcaggcaat aacaaatttt tcagcggtta gtgtttcaac
    34081 gctgccgtcc gggcaatcca gcgccaacgt atgctcgtca acaaagcgag cgttcccctg
    34141 caatatttca cagtgattac gttcgtaaaa tccctgacgc atgcgcgttt gttgattaat
    34201 cacgttatcg gcatggttaa ggatatcggc aaaagaagag cggagcagtc gggaatggtc
    34261 gctgtatagt gggttttgat tgaattctat aatgcggctg acggcgtgac ggagagcttt
    34321 cgacgggatg gtgccccagt gggtgcaacc gccgccaaca ttttgataac gctcgataac
    34381 tgcgacgcgc gcaccttgct taaccaggcc cattgcagcg ccttcgccgc cggggccgga
    34441 acctattact atggcatcgt aatcgtagga atgtggcatg gtagggctta cctgttctta
    34501 tacataaaag caacagaatg gtaacatttt atcgcgggta agccaattga tccccgtcat
    34561 taatctggct atagcgtgag cggcctttgc tttgtctgcc tccttcttaa cttatttatt
    34621 cactattaat cgctttttct tatggctgat agttttatct tgccgaagcg tgctt
//
LOCUS       DAPGOF010000032        34600 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.032, whole genome shotgun sequence.
ACCESSION   DAPGOF010000032 DAPGOF010000000
VERSION     DAPGOF010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34600
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.032"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            359..580
                     /locus_tag="RHV13_001348"
     CDS             359..580
                     /locus_tag="RHV13_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1240901.1"
                     /translation="MGLWLKIFKIVVGENENYLKDVMMQLEEKNSEGKSVISKANGNP
                     VFKELFWKAIDEFNFPQEELNRLNQYRSL"
     gene            complement(728..2092)
                     /gene="mnmE"
                     /locus_tag="RHV13_001349"
     CDS             complement(728..2092)
                     /gene="mnmE"
                     /locus_tag="RHV13_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDV1240902.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGISEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            complement(2194..3840)
                     /gene="yidC"
                     /locus_tag="RHV13_001350"
     CDS             complement(2194..3840)
                     /gene="yidC"
                     /locus_tag="RHV13_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDV1240903.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            complement(3843..4100)
                     /gene="yidD"
                     /locus_tag="RHV13_001351"
     CDS             complement(3843..4100)
                     /gene="yidD"
                     /locus_tag="RHV13_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDV1240904.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            complement(4064..4423)
                     /gene="rnpA"
                     /locus_tag="RHV13_001352"
     CDS             complement(4064..4423)
                     /gene="rnpA"
                     /locus_tag="RHV13_001352"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDV1240905.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            complement(4440..4580)
                     /gene="rpmH"
                     /locus_tag="RHV13_001353"
     CDS             complement(4440..4580)
                     /gene="rpmH"
                     /locus_tag="RHV13_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDV1240906.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            complement(4810..4890)
                     /locus_tag="RHV13_001354"
     CDS             complement(4810..4890)
                     /locus_tag="RHV13_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1240907.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTRQPV"
     gene            5187..6590
                     /gene="dnaA"
                     /locus_tag="RHV13_001355"
     CDS             5187..6590
                     /gene="dnaA"
                     /locus_tag="RHV13_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDV1240908.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            6595..7695
                     /gene="dnaN"
                     /locus_tag="RHV13_001356"
     CDS             6595..7695
                     /gene="dnaN"
                     /locus_tag="RHV13_001356"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDV1240909.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            7695..8768
                     /gene="recF"
                     /locus_tag="RHV13_001357"
     CDS             7695..8768
                     /gene="recF"
                     /locus_tag="RHV13_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDV1240910.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            8797..11211
                     /gene="gyrB"
                     /locus_tag="RHV13_001358"
     CDS             8797..11211
                     /gene="gyrB"
                     /locus_tag="RHV13_001358"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDV1240911.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            11451..11849
                     /locus_tag="RHV13_001359"
     CDS             11451..11849
                     /locus_tag="RHV13_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HDV1240912.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQPVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMDLLKGKLFR"
     gene            11964..12776
                     /gene="yidA"
                     /locus_tag="RHV13_001360"
     CDS             11964..12776
                     /gene="yidA"
                     /locus_tag="RHV13_001360"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDV1240913.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(12822..13478)
                     /locus_tag="RHV13_001361"
     CDS             complement(12822..13478)
                     /locus_tag="RHV13_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1240914.1"
                     /translation="MKLNFKGFFKAASLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLKDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(13724..14788)
                     /gene="cbrA"
                     /locus_tag="RHV13_001362"
     CDS             complement(13724..14788)
                     /gene="cbrA"
                     /locus_tag="RHV13_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HDV1240915.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDNKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFATLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            14889..16103
                     /locus_tag="RHV13_001363"
     CDS             14889..16103
                     /locus_tag="RHV13_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HDV1240916.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSDKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPDGERVSFTYNDHVMHQLDSALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLGNRIACAHAQSGEVEYLTENHANLPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            complement(16105..16437)
                     /locus_tag="RHV13_001364"
     CDS             complement(16105..16437)
                     /locus_tag="RHV13_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HDV1240917.1"
                     /translation="MIRNVLLAFMICSGMALLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     misc_feature    16677..16749
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            16743..17156
                     /gene="ibpA"
                     /locus_tag="RHV13_001365"
     CDS             16743..17156
                     /gene="ibpA"
                     /locus_tag="RHV13_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HDV1240918.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            17268..17696
                     /gene="ibpB"
                     /locus_tag="RHV13_001366"
     CDS             17268..17696
                     /gene="ibpB"
                     /locus_tag="RHV13_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HDV1240919.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            17892..19553
                     /locus_tag="RHV13_001367"
     CDS             17892..19553
                     /locus_tag="RHV13_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HDV1240920.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(19550..20266)
                     /locus_tag="RHV13_001368"
     CDS             complement(19550..20266)
                     /locus_tag="RHV13_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDV1240921.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            20562..22178
                     /locus_tag="RHV13_001369"
     CDS             20562..22178
                     /locus_tag="RHV13_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDV1240922.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSTENVAITEAV"
     gene            22178..23500
                     /locus_tag="RHV13_001370"
     CDS             22178..23500
                     /locus_tag="RHV13_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDV1240923.1"
                     /translation="MTKFSVVVAGGGSTFTPGIVLMLLANQDRFPLRALKFYDNDGAR
                     QEVIAEACKVILKEKAPDIAFSYTTDPEVAFSDVDFVMAHIRVGKYPMRELDEKIPLR
                     HGVVGQETCGPGGIAYGMRSIGGVLELVDYMEKYSPNAWMLNYSNPAAIVAEATRRLR
                     PNAKILNICDMPIGIESRMAQIVGLQDRKQMRVRYYGLNHFGWWTSIEDLQGNDLMPQ
                     LRQYVSKHGYVPPQQDTHTEASWNDTYAKARDVQALDPDTLPNTYLKYYHFPDYVVQH
                     SNPEHTRANEVMEHREKQVFDACRAITAAGNSAAGKLEIDEHASYIVDLATAIAFNTQ
                     ERMLLIVPNNGAIHNFDDEAMVEIPCLVGHNGPEPLVVGDIPQFQKGLMSQQVAVEKL
                     VVDAWEQRSYQHLWQAITLSKTVPSASVAKAILDELLEANKAYWPELR"
     gene            complement(23497..24390)
                     /locus_tag="RHV13_001371"
     CDS             complement(23497..24390)
                     /locus_tag="RHV13_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDV1240924.1"
                     /translation="MNGKLQTSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            24557..26272
                     /locus_tag="RHV13_001372"
     CDS             24557..26272
                     /locus_tag="RHV13_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HDV1240925.1"
                     /translation="MNSLQILSFVGFTLLVAIITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIASVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            26269..27762
                     /locus_tag="RHV13_001373"
     CDS             26269..27762
                     /locus_tag="RHV13_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDV1240926.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDVGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARTEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYELGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDARPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(27809..28258)
                     /locus_tag="RHV13_001374"
     CDS             complement(27809..28258)
                     /locus_tag="RHV13_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1240927.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTFESIV
                     ADKTAEIKRGLVAGIKGEKITTVEKKEDVDVDKILNQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            28367..28714
                     /locus_tag="RHV13_001375"
     CDS             28367..28714
                     /locus_tag="RHV13_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HDV1240928.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            28704..29066
                     /locus_tag="RHV13_001376"
     CDS             28704..29066
                     /locus_tag="RHV13_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HDV1240929.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            29063..29560
                     /locus_tag="RHV13_001377"
     CDS             29063..29560
                     /locus_tag="RHV13_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDV1240930.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDTLQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDMTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     HPRRDCQACDWHRLCPHAREETPDNVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(29568..30752)
                     /gene="emrD"
                     /locus_tag="RHV13_001378"
     CDS             complement(29568..30752)
                     /gene="emrD"
                     /locus_tag="RHV13_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HDV1240931.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILIGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNGGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(31171..31260)
                     /gene="tisB"
                     /locus_tag="RHV13_001379"
     CDS             complement(31171..31260)
                     /gene="tisB"
                     /locus_tag="RHV13_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HDV1240932.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            31825..31923
                     /gene="ivbL"
                     /locus_tag="RHV13_001380"
     CDS             31825..31923
                     /gene="ivbL"
                     /locus_tag="RHV13_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HDV1240933.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            32029..33717
                     /gene="ilvB"
                     /locus_tag="RHV13_001381"
     CDS             32029..33717
                     /gene="ilvB"
                     /locus_tag="RHV13_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HDV1240934.1"
                     /translation="MASSGTSSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPLVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYKQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            33721..34011
                     /gene="ilvN"
                     /locus_tag="RHV13_001382"
     CDS             33721..34011
                     /gene="ilvN"
                     /locus_tag="RHV13_001382"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HDV1240935.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            34275..>34600
                     /locus_tag="RHV13_001383"
     CDS             34275..>34600
                     /locus_tag="RHV13_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001626294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1240936.1"
                     /translation="MASPINSGTMMSNPSPSTFLDKNRIIAAELNITNNEKKYSPGSN
                     FAKWMLQEIKRLIANIMSGSRSVNTEILNYFHPMPGTENNGNRTWMAATGENEYTVIE
                     QTGDKS"
BASE COUNT         8343 a         8778 c         9146 g         8333 t
ORIGIN      
        1 gacgggggag tcagacaaat ggacgaaggc atcaggctta tattctgcga tagtgaaagg
       61 gtatacggag attgttgccg cctggatgga gacagctgaa gtgatagcca gccattatga
      121 aaatgataaa gatgtagtga gagaactcct gtcgctgagc agaaataatg ctgtctgctc
      181 tttgcgtatt gcaagcttta agaaaatgag taaacaggtc attgacgtat atctgaatgc
      241 ggcaattcgc ctggcgttgc aacacgggtt cactttcgat gagattttgg agcagtttac
      301 ccgtgacttt gatgggaagt cattctctca tgtggtaact aacgaggatg atatacatat
      361 gggactctgg ctgaaaatat tcaaaattgt ggttggtgaa aatgaaaatt accttaaaga
      421 tgtgatgatg caactggaag aaaagaatag tgaaggcaaa tctgtaatca gtaaggcaaa
      481 tggcaatcct gtatttaaag aactattctg gaaagcaata gacgaattta actttccgca
      541 agaggagctt aatcggttaa accaatatcg ttctctgtaa agacaggact gcaagatgtt
      601 gaaagtcgct ccagccagta gcaaaggcag ttttggtgca ggatgctcat aaaaaacaag
      661 cgcagttccg ttctgatagt tagtttaccc agaacggaac agtgcccttt atatcaattt
      721 agacgactta cttaccaata cagaagctgg agaaaatccg ccccagcagg tcgtctgaag
      781 taaattcccc ggtgatttcg cttaagttct gctgtgccag gcgcaactct tccgccagta
      841 gttcgcctgc ccaggcacct aacagttgcg ctttaccttg ctgcaaatgc tccgctgcct
      901 gttccagcgc ctgtaggtgg cgacggcgcg ccaggaagcc gccttccatg ttggtgtcaa
      961 agcccatgct ctgtttgaga tggttacgca gcacgtccac gccttcgcca gtccttgcag
     1021 acagacgaat taacgcgtga ccgttcactt cacttattcc cagcgtttcg ccggtgatat
     1081 cggctttatt gcgcaccacg gtgatcggca gtttcgctgg cagacgggcg ataaattccg
     1141 gccagatctc tgccggatca acggcgtctg tcgtggtgcc atcgaccata aacagcacgc
     1201 ggtcggcctg ttcaatttcc tgccacgcgc gctcgatccc aatacgttca acttcgtcac
     1261 tggcttcacg tagcccggcg gtatcgatga tatgcagcgg cattccgtca atatggatat
     1321 gctcacgcag cacgtcacgc gtggttccgg cgatatcggt tacgattgcc gcttcacggc
     1381 ccgccagcgc gtttaacagg ctcgatttac cggcattagg acgtccggca atcaccacct
     1441 tcatcccttc gcgcaacaaa ctaccctgac gtgcttcagc acgcactgca tcgagatcgg
     1501 caatcacgtc attaagctgg gcttcaattt tgccgtcgga gaggaaatcg atctcttcat
     1561 cggggaaatc aattgccgct tcaacgtaaa tgcgcaagtg ggtgagggct tctaccagat
     1621 gattaacccg tgcggagaat gcgccttgca gcgagttaag tgccgaacgg gctgcctgtt
     1681 ccgaactggc gtcgataaga tccgcaatcg cctcagcttg ggctaagtcg agtttatcgt
     1741 taagaaacgc gcgttcggaa aactcaccag ggcgagcaat ccgcaggccg ggaatggtca
     1801 gaatgcgttt taacagcagg tcgaggatca ccggaccgcc atgaccttgc agttccagca
     1861 catcttcgcc ggtgaacgag ttcgggccag ggaaccatag cgcaatcccc tgatcgagca
     1921 cgctgccgtc ggcgtcttta aacggaagat aatcggcgta gcgcggctta ggcagtttac
     1981 ccagcacggt ttcggcaact tcacgggctt tgaagccgga gatgcgcagg atgccaacgc
     2041 cgccacgtcc cggaggcgtg gcctgggcta cgatagtgtc attatcgctc atgatgttcc
     2101 tgttgctttg tgtggcggat gcgcggtatc cgccctacga aaagaaaaaa ggcggtcaac
     2161 tgaccgccct tattttagcg aaaactcacc gaatcaggat tttttcttct cgcggctatg
     2221 caggccacgt ttttccagac cacggtaaat cagctgctgc tggataatgg ttaccaggtt
     2281 gctgacgata tagtacaaca ccagacctga cgggaaccac aggaagaaca cggtgaagat
     2341 gaccggcata aaggtcatga tcttctgctg catcgggtcg gtcacggtgg tcggcgacat
     2401 cttctgaatg aagaacatcg ttacgcccat caggatcggc aggatgtagt acgggtcctg
     2461 tgcagacagg tcgtggatcc acagtgcgaa cggcgcctga cgcagttcaa cggaacccat
     2521 cagcatgtag tacaacgcca ggaagattgg catctggatc agcagcggga agcagccgcc
     2581 cagcgggtta accttctcag ctttgtacag cgccatcatt tcctggctga tacgttgttt
     2641 gtcatcgccc agacgctcac gcattgcctg aatcttcggc tgcagcatac gcatcttcgc
     2701 catggaggtg tactgcgctt tggtcagcgg gtacatgatg ccacgaacga taaaggtgat
     2761 gatgataatg gagaagcccc agttacccac aaagctatgg atccatttca gcagtttgaa
     2821 cagcggctga gagatgaacc acaaccaacc gtaatcaacg gtcagatcca ggtgcggagc
     2881 aacagctgcc attttgtcct ggatttccgg gccaacccac agggtgctgt tcatcgcgcc
     2941 agtctgacca ggctgaacca gtaccggctg agatttatag ccgatagcgg cgatgccgtt
     3001 acccagatta gcggtataga agttgttggt accgtcgtta tgcgggatcc acgccgtcgc
     3061 gaaatactgt tgcagcatcg ccacccaacc acctttcgaa gagatgttca ggttttcgtt
     3121 atcggcaatg gtatcgaact tgtatttctc atacttctcg tcaggcgtgg agtacgccgc
     3181 gccacggaag gtgtgcagtg cgaagttgct gcttccggta tcgagatgcg gtggcagagt
     3241 gatggattgc ttcaactgac caaacgagga gatttccagc ggtttctcgc cagcgttctg
     3301 cacgttgtag ttgacgttga cagcgtaatc accacgtttc aggacaaacg ttttggtaaa
     3361 cgtgttgcct gccgcgtcgg tatacgtcat cggcacctgc agttcgtttt gaccttcagc
     3421 cagcacataa gcgtcttttt caacgttata cagcggacgc gggccgttag ccgggttatc
     3481 tgggccatca cgaccggtca gaccgctctg tgcctgataa ataaactgcg gtgaagtttc
     3541 cagcagctgg aacggctggg tagagttcag ctctttcggg taagccggca gcagggcctg
     3601 ctcaacatca ccaccacggg tgttgatggt cagatcaagc acgtcggtct taaccgagat
     3661 cagtttcccc tggccactgg ccggtacgcc ctggtcggcg gcgctacccg ctgcggtggt
     3721 cgttgtctgc gtggtctgtt gagcttgagg ttgcggattt ttatcctgct cccaggcttg
     3781 ccagatcatg aaagacacga acagcaaagc gatgactaaa agattgcgtt gcgaatccat
     3841 cgttagtgtt ctctggtatc aaatggtccg ggcgggacgg gatcgtcacc accagggtgt
     3901 aaagggtggc attttaatac gcgtttcacc gtcaaccaac tgccttttat cactccaaac
     3961 ctgcgcaatg cctcaattcc gtagcttgaa caggttggag tgaaacgaca atgcggcccg
     4021 agtagcggac taatcaggcg ttgatagacc cgaatgaggg ctatcaggac ccgcgagcca
     4081 ggcgacagtg gcggcgccat aatttttcca acgcttccga gagagcacgg ttatcgaggt
     4141 cggcaacccc ttttttcgcc accaccacga aatccatagc cgggagttca tgttggcgca
     4201 gacggaagct ttcacgcgtc agacgtttaa tccgattgcg ttcatgggcg cgtcgaacgt
     4261 ttttcttggc gactgtaaga ccgatacggg gatgccccag cgaattcagg cggccgagaa
     4321 tggtaatttg cggcgtgcca gcccgttgtg gctgctggaa gacgaatgtg aattgactgg
     4381 gagttaacaa gcgtaactcc ctgggaaatg cgagcttaac cactcagggg ttagctttat
     4441 tacttagaaa cggtcagacg agcgcggcct ttagcacgac gacgtgccag aacctgacga
     4501 ccatttttag tagccatacg agcacggaag ccgtgagaac ggttgcgctt cagtacagac
     4561 ggttgaaaag tgcgtttcat ggcgatttct acctaaactt gaataaattc aatggcttta
     4621 ttggatatcc gccgaaaaat gaaacgatgg acaccgaagc catgggtgat taaagaggcc
     4681 ggattgtaat aattgtacac tccggagtca attctctttc cttatttacc gcgcttttcc
     4741 gcaccttttc gcagggaaaa cgtacggcct cacaccagtg aaaaccagca tggcgcgccg
     4801 ggtggaggat tatacgggct gacgggtaaa gcgcaaggat cgtcctggat ctttattaga
     4861 tcgattaagc caatttttgt ctatggtcat taaattttcc aatatgcggc gtaaatcgtg
     4921 cccgcctcgc ggcaggatcg tttacactta gcgagttctg gatcatcctg tggataaatc
     4981 gggaaaatct gtgagaaaca gaagatctct tgcgcagttt aggctatgat ccgcggtccc
     5041 gatcgttttg caggatcttg atcgggcata taaccgcaga cagcggttcg tgcgtcaccc
     5101 tcaagcaggg tcttttcgac gtacgtcaac aatcatgaat gtttcagcct tagtcattat
     5161 cgacttttgt tcgagtggag tccgccgtgt cactttcgct ttggcagcag tgtcttgccc
     5221 gattgcagga tgagttacca gccacagaat tcagtatgtg gatacgccca ttgcaggcgg
     5281 aactgagcga taacacgctg gccctgtacg cgccaaaccg ttttgtcctc gattgggtac
     5341 gggacaagta ccttaataat atcaatggac tgctaaccag tttctgcgga gcggatgccc
     5401 cacagctgcg ttttgaagtc ggcaccaaac cggtgacgca aacgccacaa gcggcagtga
     5461 cgagcaacgt cgcggcccct gcacaggtgg cgcaaacgca gccgcaacgt gctgcgcctt
     5521 ctacgcgctc aggttgggat aacgtcccgg ccccggcaga accgacctat cgttctaacg
     5581 taaacgtcaa acacacgttt gataacttcg ttgaaggtaa atctaaccaa ctggcgcgcg
     5641 cggcggctcg ccaggtggcg gataaccctg gcggtgccta taacccgttg ttcctttatg
     5701 gcggcacggg tctgggtaaa actcacctgc tgcatgcggt gggtaacggc attatggcgc
     5761 gcaagccgaa tgccaaagtg gtttatatgc actccgagcg ctttgttcag gacatggtta
     5821 aagccctgca aaacaacgcg atcgaagagt ttaaacgcta ctaccgttcc gtagatgcac
     5881 tgctgatcga cgatattcag ttttttgcta ataaagaacg atctcaggaa gagtttttcc
     5941 ataccttcaa cgccctgttg gaaggtaatc aacagatcat tctcacctcg gatcgctatc
     6001 cgaaagagat caacggcgtt gaggatcgtt tgaaatcccg cttcggttgg ggactgactg
     6061 tggcgatcga accgccagag ctggaaaccc gcgtggcgat cctgatgaaa aaggccgacg
     6121 aaaacgacat tcgtttgccg ggcgaagtgg cgttctttat cgccaagcgt ctacgatcta
     6181 acgtacgtga gctggaaggg gcgctgaacc gcgttattgc taacgccaac tttaccggac
     6241 gggcgatcac catcgacttc gtgcgtgagg cactgcgcga cttgctggca ttgcaggaaa
     6301 aactggtcac catcgacaat attcagaaga cggtggcgga gtactacaag atcaaagtcg
     6361 cggatctcct ttccaagcgt cgatcccgct cggtggctcg tccgcgtcag atggcgatgg
     6421 cgctggcgaa agagctgact aaccacagtc tgccagagat tggcgatgcg tttggtggtc
     6481 gtgaccacac tacggtgctt catgcctgcc ggaagatcga gcagctgcgt gaagagagcc
     6541 acgatatcaa agaagatttt tcaaatttaa tcagaacatt gtcatcgtaa acctatgaaa
     6601 tttaccgtag aacgtgagca tttattaaaa ccgctacaac aggtgagcgg tccgttaggt
     6661 ggtcgtccta cgctaccgat tctcggtaat ctgctgttac aggttgctga cggtacgttg
     6721 tcgctgaccg gtactgatct cgagatggaa atggtggcac gcgttgcgct ggttcagcca
     6781 cacgagccag gagcgacgac cgttccggcg cgcaaattct ttgatatctg ccgtggtctg
     6841 cctgaaggcg cggaaattgc cgtgcagctg gaaggtgagc ggatgctggt gcgctccggg
     6901 cgtagccgtt tttcgctgtc taccctgcca gcggcggatt tcccgaacct cgatgactgg
     6961 cagagtgaag tcgaatttac cctgccgcag gcgacgatga agcgtctgat tgaagcgacc
     7021 cagttttcta tggcgcatca ggacgttcgc tattacttaa acggtatgct gttcgaaact
     7081 gaaggtgaag aactgcgcac cgtggcgacc gatggtcacc gtctggcggt ctgttcaatg
     7141 ccaattggtc aatctttgcc aagccattcg gtgatcgtgc cgcgtaaagg cgtgattgaa
     7201 ctgatgcgta tgctcgacgg cggcgacaat ccgctgcgcg tgcagattgg cagcaacaat
     7261 attcgcgccc acgttggcga ctttatcttc acctctaaac tggtggatgg tcgcttcccg
     7321 gattaccgcc gcgttctgcc gaagaatccg gacaagcatc tggaagcagg ctgcgatctc
     7381 ctcaagcagg cgtttgctcg cgcggcaatt ctctctaacg agaaattccg cggtgtgcgc
     7441 ctgtatgtca gcgaaaacca gctgaaaatt actgccaaca acccggaaca ggaagaagcg
     7501 gaagagatcc tcgacgttac ctacagcggt gcggagatgg aaatcggctt caacgtcagc
     7561 tatgtgctgg atgtcctgaa cgcgctgaaa tgcgaaaacg tccgcatgat gctgaccgat
     7621 tcggtttcca gcgtgcagat tgaagatgca gccagccaga gcgcggctta tgttgtcatg
     7681 ccaatgagac tgtaatgtcc ctcacccgct tgttgatccg cgatttccgc aacattgaaa
     7741 ccgcggatct cgctttatct cccggcttta actttctggt aggtgccaac ggcagtggca
     7801 aaaccagcgt gctggaagcc atctatacgc tcggccatgg tcgggcgttt cgcagtttgc
     7861 agattggtcg cgtcattcgc catgagcagg aggcgtttgt tctccacggg cgtttgcagg
     7921 gcgaagagcg cgagacggcg attggcttaa ccaaagacaa acagggcgac agcaaagtcc
     7981 gcattgacgg tactgacggg cataaggtgg cggaactggc gcacctgatg ccgatgcaac
     8041 tgataacgcc agaagggttt actttactca acggcggccc caaatacaga agagcattcc
     8101 tcgactgggg atgctttcac aacgaacccg gatttttcac cgcctggagc aatctcaagc
     8161 gattgctcaa gcagcgcaat gcggcgctgc gccaggtgac acgttacgaa cagctacgcc
     8221 cgtgggataa agaactgatc ccgctggcgg agcaaatcag tacctggcgc gcggagtata
     8281 gcgccggtat cgcggccgat atggctgata cctgtaagca atttctccct gagttttctc
     8341 tgactttctc tttccagcgc ggctgggaga aagagacaga atatgctgag gtgctggaac
     8401 gtaattttga acgcgatcgc cagctaacct acaccgcgca tggcccgcat aaagcggact
     8461 tacgcattcg cgccgacggt gcgccggtgg aagatacctt atcgcgtggg cagcttaagc
     8521 tgttgatgtg cgccttacgt ctggcgcaag gagagttcct cacccgtgaa agcgggcgac
     8581 ggtgtctcta cctgatagat gattttgcct ctgagcttga tgatgagcgt cgcgggctgc
     8641 ttgccagccg cttaaaagcg acgcaatcgc aggtctttgt cagcgcgatc agtgctgaac
     8701 acgttataga catgtcggac gaaaattcga agatgtttac cgtggaaaag ggtaaaataa
     8761 cggattaacc caagtataaa tgagcgagaa acgttgatgt cgaattctta tgactcctcc
     8821 agtatcaaag tcctgaaagg gctggatgcg gtgcgtaagc gcccgggtat gtatatcggc
     8881 gacacggatg acggcaccgg tctgcaccac atggtattcg aggtggtaga taacgctatc
     8941 gacgaagcgc tcgcgggtca ctgtaaagaa attatcgtca ccattcacgc cgataattct
     9001 gtctctgtac aggatgacgg gcgcggcatt ccgaccggta ttcacccgga agagggcgta
     9061 tcggcggcgg aagtgatcat gaccgttctg cacgcaggcg gtaaatttga cgataactcc
     9121 tataaagtgt ccggcggtct gcacggcgtt ggtgtttcgg tagtaaacgc cctgtcgcaa
     9181 aaactggagc tggttatcca gcgcgagggt aaaattcacc gtcagatcta cgaacacggt
     9241 gtaccacagg ctccgctggc ggttaccggc gagactgaaa aaaccggcac tatggtgcgt
     9301 ttctggccca gcctcgaaac cttcaccaat gtgaccgagt ttgaatatga aattctggcg
     9361 aaacgtctgc gtgagttgtc gttcctcaac tccggcgttt ccattcgtct gcgcgacaag
     9421 cgtgatggca aagaagacca cttccactat gaaggcggca tcaaagcgtt cgttgaatat
     9481 ctgaacaaga acaaaacgcc gatccaccca aatatcttct acttctccac cgaaaaagac
     9541 ggtattggcg ttgaagtggc gctgcagtgg aacgatggct tccaggaaaa catctactgc
     9601 tttaccaaca acattccgca gcgtgacggc ggtactcacc tggcaggctt ccgtgcggcg
     9661 atgacccgta ccctgaatgc ctacatggac aaagaaggct acagcaaaaa agccaaagtc
     9721 agcgccaccg gtgatgatgc gcgtgaaggc ctgattgcgg tcgtttccgt aaaagtgccg
     9781 gacccgaaat tctcctccca gaccaaagac aaactggttt cttctgaggt gaaatcggcg
     9841 gttgaacagc agatgaacga actgctggcg gaatacctgc tggaaaaccc aactgatgcg
     9901 aaaatcgtgg ttggcaaaat tatcgatgct gcccgtgccc gtgaagctgc gcgtcgcgct
     9961 cgtgaaatga cccgccgtaa aggtgcgctc gacttagcgg gcctgccggg caaactggca
    10021 gactgccagg agcgcgatcc ggcgctttcc gaactgtacc ttgtggaagg ggactccgcg
    10081 ggcggctctg cgaagcaggg gcgcaaccgc aagaaccagg cgattctgcc gctgaagggt
    10141 aaaatcctca acgtcgagaa agcgcgcttc gataagatgc tctcttctca ggaagtggcg
    10201 acgcttatca ccgcgcttgg ttgtggtatc ggtcgtgacg agtacaaccc ggacaaactg
    10261 cgttatcaca gcatcatcat catgaccgat gcggacgtcg acggctcgca cattcgtacg
    10321 ctgctgttga ccttcttcta tcgtcagatg ccggaaattg ttgaacgcgg tcacgtctac
    10381 atcgctcagc cgccgctgta caaagtgaag aaaggcaagc aggaacagta cattaaagac
    10441 gacgaagcga tggatcagta ccaaatctcc atcgcgctgg acggcgcaac gctgcacacc
    10501 aacgccagtg caccggcatt ggctggcgaa gcgttagaga aactggtatc tgagtacaac
    10561 gcgacgcaga aaatgatcaa ccgcatggag cgtcgttatc cgaaagcaat gctgaaagag
    10621 cttatctatc agccgacgct gacggaagct gacctctctg atgagcagac cgttacccgc
    10681 tgggtgaacg cgctggtcag cgaactgaac gacaaagaac agcacggcag ccagtggaag
    10741 tttgatgtcc acaccaatgc cgaacaaaac ctgtttgaac cgattgttcg cgtgcgtacc
    10801 cacggtgtgg atactgacta tccgctggat cacgagttta tcaccggtgg cgaatatcgt
    10861 cgtatctgca cgctgggtga gaaactgcgt ggcttgctgg aagaagatgc gtttatcgaa
    10921 cgtggcgagc gtcgtcagcc ggtagccagc ttcgagcagg cgctggactg gctggtgaaa
    10981 gagtcccgtc gcggcctctc catccagcgt tataaaggtc tgggcgagat gaacccggaa
    11041 cagctgtggg aaaccaccat ggacccggaa agccgtcgta tgctgcgcgt taccgttaag
    11101 gatgcgattg ccgctgacca gttgttcacc acgctgatgg gcgacgccgt tgaaccgcgc
    11161 cgtgcgttta ttgaagagaa cgccctgaaa gcggcgaata tcgatattta atggcgttaa
    11221 ccatgcgagc gtgcctgttg cgctacgctt atcaggccta cgagactcct gcaatatatt
    11281 gaatttgcat gtttttgtag gccggataag gcgttcacgc cgcatccggc aagaagctat
    11341 aagaaaaggg cggagatcat ctccgccctt tttatttctg caatccgcaa ttatcatccc
    11401 gcagttctat actttttggg tcaacacgta acgctttaca ggtgattatc atggggcttt
    11461 ttgatgaagt tgtcggcgcc tttctgaaag gcgatgcggg gaaatatcag gcgattttaa
    11521 gttgggttga ggagcagggt ggcattcagg tgctgctgga aaaactgcaa agtggcggct
    11581 taggggccat tctctcaacc tggctgagta atcaacaggg caatcaacca gttagcggcg
    11641 agcaactgga atcagcactc ggcacaaacg cggtgtccga tctcgggcaa aaacttggcg
    11701 tggataccag tacagcttcc agtttactgg cagaacaatt gccgaagatt attgatgcgc
    11761 tctcaccgca aggtgaagtg tcaccacaag ccaataacga tctgctttcc gcaggcatgg
    11821 atctgctaaa agggaaactc ttccgctaag caaaagggga gcacgcaacg cgcgttgcat
    11881 cccccttttc cccagggtgt tgtactgatt tttgagcgga atcgcgttag catgggtcag
    11941 gaaccaatct acctggggaa ctcatggcta tcaaactcat tgctatcgat atggatggca
    12001 cccttctgct gcccgatcac accatttcac ccgccgttaa aaatgcgatt gccgcagctc
    12061 gcgcccgtgg cgtaaatgtc gtgctgacga cgggccgccc ttatgcaggt gtgcacaact
    12121 acctgaaaga gctgcatatg gaacagccgg gcgactactg cattacttac aacggtgcgc
    12181 tggtgcagaa ggccgctgat ggtagcaccg tggcgcaaac cgctctcagc tatgacgact
    12241 accgtttcct ggaaaaactc tctcgcgaag tcggttctca tttccacgcc ctggaccgca
    12301 ccacgctgta caccgccaac cgtgatatca gctactacac ggtgcatgaa tccttcgttg
    12361 ccaccattcc gctggtgttc tgcgaagcgg agaaaatgga ccccaatacc cagttcctga
    12421 aagtgatgat gattgatgaa cccgccatcc tcgaccaggc tatcgcgcgt attccgcagg
    12481 aagtgaaaga gaaatatacc gtgctgaaaa gtgcgccgta cttcctcgaa atcctcgata
    12541 aacgcgttaa caaaggtacg ggggtgaaat cactggccga cgtgttaggt attaaaccgg
    12601 aagaaatcat ggcgattggc gatcaggaaa acgacattgc aatgattgaa tacgcaggcg
    12661 tcggtgtggc aatggataat gctattcctt cggtgaaaga agtggcgaac tttgtcacca
    12721 aatccaacct tgaagatggc gtggcgtttg ccattgaaaa gtatgtgctg aattaatctg
    12781 tgggcgggca aacagcataa acgcgtttgc ccgcttactg attattggtt tactgcatat
    12841 acaaccagaa attgcagcgg tgcagtaatg atgtccagcg taacagtcac tggcagtaat
    12901 gctgcggaaa caccatccgg aatgcctctg ggtgaatagt attcataaaa tcgcacttta
    12961 aatggatggt agaacgccat cacctttgag taatccatgt ttttattttt ctcatggatt
    13021 gtgcctttta ggttttccag tgacctggta cagtgttgaa caccacaggc aaaaccataa
    13081 tgcgttgcca gtgccttttc ttcttcgtta ttccagtagt agtaaagcga tattgtgccg
    13141 gtaaatttct ttttccccgc atttaaaaca aatcttgcgt cgtcagcgac ctgaatatta
    13201 tgcctgttaa gagctggatc cttgagcatc ttaacgatgt catcggcacc atcagtgata
    13261 aggtaatcca gcgattcccc tacaaaaaca tagccttttg ttccattact atcttttgcc
    13321 tgcgatagcc cagtgatggt gtctgactgc gattgatact ttcctgaact accctttgcg
    13381 gtatgggaaa ccagagcata cgagatacag cctgaaagca tcagcgccag tgggaataaa
    13441 ctggcagcct taaaaaatcc cttaaaattc aacttcatga ttaatccttt aaacgagtag
    13501 ctaagccgtt gaaagcatac tcttctttcc ctcctgttta gcgcctgatc tcccttttca
    13561 gatttctgcc cgatataaga ccttttctta tgaggtgttt ttttgtggac taatatctgt
    13621 tgcaaggtta tgggttcgcc ccggcactgg tttatatctc cgccgtcgcg ttgattggcg
    13681 cgctctctta catcctgctg gtgggcgatg tgaagcgcgt tggctaatcc ttcaactgtg
    13741 gaatatgtgc cacaccactg cgcataatcc actttcttaa agccggtgcg gtcaggcatc
    13801 ggctttttac tatcttgccg aagagtttta aacgcagttt gcgggtggcg cgccagtaag
    13861 cggtattgag cttctctggc tgcttcagta acaccgaacg cagaatgtct gcgctatcca
    13921 gcgcatagct aatcccctcc agcgagctgg cgctgataaa tcccgccgct tcaccaatca
    13981 gaaaggcgtt gtccttaccg cagacaaaat cctgccagcg tgagggaaac agcactgtgc
    14041 atttttcgct tttcaccgct ttgccaaact ggaactgaaa ggcgctcatt ttctctttca
    14101 gcgtcgcgaa acgcgtctga ccgtctttca ttggataggc accgccaaag ataaaataac
    14161 cgtctttgct gatactccat gagtaacagt cagttatcgc attatcaaag atgcaggagt
    14221 agaacggcac cggatgtttc tctgcgaacc actgctggat tgcgacatat ttacggattt
    14281 gatgatccgg gtagagatgt cgccgcacca tcgagttggc accatcggca ccgaccagat
    14341 agcgggcggt aatatgctgc tcccagccgt ctgcacgaaa aatgacatgc catttattat
    14401 cctcacgcca gattttccgg cacaggctat cgtggtaaac ctcaacgctg gcggggatca
    14461 gcgatttcat ccacaagtcg aaagcatggc gattaatatt gatatagctt cgctggtagt
    14521 tacgcgtgag cgatgcggcg acgtcgacgg ttttgacgct gaaaatctgc ggattagcga
    14581 tcacatcgac aggaagcgtc agcccatcgc gaataaaaga tcgctgcgcg tccggtgcca
    14641 gcagaccgcc acaaggtttg ctgaaacctt cagtaccatg ctggtgcttt ttatccagcg
    14701 cgatcacctg cattttgcct gctaactttc gtgccaacgc cgatcctgcc gggccgaggc
    14761 caataatcgc cacgtcgaaa tgttccatct tttcactcac attcatcacg atggcaaaag
    14821 actattgtct gaaagtgaag tccttgtgaa gtatgatggc gggaaccgtt ttgtatcagg
    14881 accacgccat gaaacagata acctttgctc cccgcaatca cctgctcact aataccaata
    14941 cctggacgcc cgacagccag tggctggtat ttgacgtgcg tccttctggg gcgtcgttta
    15001 ccggcgagac cattgagcgt gtgaatatcc ataccggcga ggtcgaggtt atctatcgcg
    15061 cgtcacaggg cgcacacgtc ggcgtggtga ccgttcatcc gaagtcagat aaatatgtct
    15121 ttattcacgg ccctgaaaat cctgatgaaa catggcatta cgatttccat catcgacgcg
    15181 gagtcattgc tgaaggcggc aaggtgagca atctcgatgc aatggatatt actgcaccgt
    15241 acaccccagg agcgctgcgc ggcggcagcc atgtacatgt ctttagcccg gacggtgaaa
    15301 gggtgagctt tacctataac gaccatgtaa tgcatcagct cgattcggcg ctggatttgc
    15361 gaaacgtcgg tgttgctgca ccgtttggcc cggtcaatgt acaaaagcaa catccgcgtg
    15421 aatacagcgg tagccactgg tgtgtgctgg tgagtaaaac cacgcccacg ccgcagcctg
    15481 gcagtgatga aatcaatcgt gcttatgaag aaggatgggt aggaaatcac gcgctggcat
    15541 ttattggcga cacactttcg ccaaagggcg agaaagtgcc ggagctgttt atcgttgagt
    15601 taccgcaaga tgaagctggc tggaaagcag caggtgatgc gccgttaagc ggaacggaaa
    15661 caaccctgcc cgcgccaccg cgtggcgtcg tgcagcgacg tttaaccttt acccaccatc
    15721 gggcttatcc ggggttagtc aacgtcccgc gccactgggt gcgctgtaat ccgcagggta
    15781 cgcaaatcgc gtttttaatg cgtgatgata acggcattgt gcaactgtgg cttatctcgc
    15841 cacagggcgg cgagccgcgc cagttaaccc ataacaaaac ggatattcag tctgcattta
    15901 actggcatcc gtcaggagaa tggttgggct ttgtgctggg taatcgaatt gcttgtgccc
    15961 atgcgcaaag cggcgaggtt gagtatttaa ccgaaaacca cgccaatcta ccttctgcgg
    16021 acgccgtggt cttctcgccg gatggtcagt ggctggcgtg gatggaaggc ggccagctgt
    16081 ggatcaccga aactgatcgc taaatcagtt atcaggcatt ctggccggtg gaataaccgc
    16141 attggtggct tgcgcgttgg cttcgctttt ttcaacgcgt gagcgcaccg agctgtcttt
    16201 gcggaacaca tcccacggca gtaaaagcgt gtccatcaca gcggtaaacg gcatatcgag
    16261 aatcgccagc gacttagtgc cccagttggt ttcatcatcg ccaatcatgg tggcgctggc
    16321 gcgggtgccg ggatacgttc cttctttccc tccggtatgg gacatcacgc tggagcatcc
    16381 tccgagtaat gccattccac tgcatatcat gaacgctaac aacacatttc ttatcataat
    16441 aaattcatct gttgatcgtg ggtgttggcc tgatgagtta tagcgatccc ttgctgaaaa
    16501 taacatcatc attacgtcgc tctgtggcgg caatcgcact ttaacgtttc gtgctgcccc
    16561 ctcagtctat gcaatagacc ataaactgca aaaaaaagtc cgctgataag gcttgaaaag
    16621 ttcatttcca gacccatttt tacatcgtag ccgatgagga cgcgcctgat gggtgttctg
    16681 gctacctgac ctgtccattg tggaaggtct tacattctcg ctgatttcag gagctattga
    16741 ttatgcgtaa ctttgattta tccccgcttt accgttctgc tattggattt gaccgtttgt
    16801 ttaaccactt agaaaacaac cagagccaga gtaatggcgg ctaccctccg tataacgttg
    16861 aactggtaga cgaaaaccat taccgcattg ctatcgctgt ggctggtttt gctgagagcg
    16921 aactggaaat taccgcccag gataatctgc tggtggtgaa aggtgctcac gccgacgaac
    16981 aaaaagagcg cacctatctg taccagggca tcgctgaacg caactttgaa cgcaaattcc
    17041 agttagctga gaacattcat gttcgtggcg ctaacctggt gaatggtttg ctgtatatcg
    17101 atctcgaacg cgtgattccg gaagcgaaaa aaccgcgccg tatcgaaatc aactaattcc
    17161 ctaaggccgc ctggcgcggc ctgacatctc catgctcgcc gtcagggagc atatgcgaat
    17221 cttcggattt gcaggtactt actcgcttct tagaaggaga aataactatg cgtaacttcg
    17281 atttatcccc actgatgcgt caatggatcg gttttgacaa actggccaac gcactgcaaa
    17341 acgccggtga aagtcagagc ttcccgccgt acaacattga gaaaagcgac gataaccact
    17401 accgcattac ccttgcgctg gcaggtttcc gtcaggaaga tttagagatt caactggaag
    17461 gtacgcgcct gagcgtaaaa ggcacgccgg agcagccaaa agaagagaaa aaatggctgc
    17521 atcaagggct tatgaatcag ccatttagcc tgagctttac gctggctgaa aatatggaag
    17581 tctctggcgc aaccttcgta aacggtttac tgcatattga tttaattcgt aatgagcctg
    17641 aacccatcgc agcgcagcgt atcgctatca gcgaacgtcc cgcgttaaat agctaactag
    17701 ctattctctt tgccccgcca ttctggtggg gctttttttg tctttcttct taaacaatgc
    17761 gttaaatttc gactgtttaa gatatttcgg cacgttcttt cccgtgataa tgtgcgctat
    17821 aaggcaaatc tgcttcaccg attcagccat aatccgtagc aattaaaatg ttatctcaag
    17881 agaaatggac gatgagtgat atagcattaa cggtcagtat tttggctttg gtggcagtcg
    17941 tcggtttgtt tatcggcaac gtcaaatttc gcggcatagg attaggtatt ggcggcgtgc
    18001 tgtttggtgg gatcatcgtc ggccattttg tttctcaggc ggggatgaca ttaagtagcg
    18061 atatgctgca tgttattcag gaatttggcc tgatcctgtt cgtttatact atcgggattc
    18121 aggtagggcc gggcttcttt gcctcattgc gcgtctccgg attacgcctc aacctgtttg
    18181 ctgttctgat cgtcatcatc ggtggtctgg ttaccgccat cctgcataaa ctgtttgata
    18241 ttccactgcc ggtagtgctg gggattttct ccggtgcggt taccaatacg ccagcgctgg
    18301 gggcagggca gcagattttg cgcgacctgg gtacaccaat ggaaatggtc gatcagatgg
    18361 ggatgagtta cgcgatggcg tatccattcg gcatttgcgg gattttgttc accatgtgga
    18421 tgttgcgggt tattttccgc gtcaatgtcg agacagaagc tcagcagcac gagtcttcac
    18481 gcaccaatgg cggcgcgctg atcaagacta tcaatattcg cgttgagaac cctaacctgc
    18541 atgatttagc cattaaagat gtaccgattc tcaacggcga caaaattatc tgctcgcgtc
    18601 tgaaacgcga agaaacccta aaagttcctt cgccagatac cattatccaa ctgggcgatt
    18661 tgctgcatct ggtgggtcag ccagcggatt tacataatgc gcaactggtg attggtcagg
    18721 aggtcgatac ttcgctgtcc acgaaaggca ctgatttgcg cgtcgagcgt gtggtggtca
    18781 ccaatgaaaa cgtgctcgga aaacgtattc gcgacctgca ctttaaagaa cgctatgacg
    18841 ttgttatctc gcgactgaac cgtgccgggg tcgaactggt cgccagtggc gatatcagcc
    18901 tgcagttcgg cgatatcctc aacctggtgg ggcgtccgtc cgcaattgat gccgttgcca
    18961 atgtgctggg gaatgcgcag caaaaactgc aacaggttca gatgctgcca gtgtttattg
    19021 gcatcgggct tggcgtattg ttaggttcta ttcccgtctt tgtgccggga ttcccggccg
    19081 cgttgaaact ggggctggcg ggcggtccgc tgattatggc gttgatcctc gggcgtatcg
    19141 gcagtatcgg caagctgtac tggtttatgc cgccaagtgc caacctcgcg ctgcgggagc
    19201 tggggatcgt gctgttcctc tctgtcgttg gtctgaaatc tggtggggat tttgtgaata
    19261 ccctggtcaa tggcgaaggg ctaagctgga ttggttatgg tgccctgatc accgccgttc
    19321 cgctgattac tgttggcatt ctggcgcgga tgttagccaa aatgaattac ctgaccatgt
    19381 gcgggatgct ggcaggttcc atgaccgatc ctccggcgct ggcgtttgct aataatcttc
    19441 atccaaccag tggtgcggcg gcgctctctt acgccactgt ctatccgtta gtgatgttcc
    19501 tgcgcattat caccccccaa ttactggcgg tgctcttctg gagtatcggt taactctttt
    19561 ccgggtggag tcgccgtaaa tggtactcca cctggtattc gctggcattt ctgaacatca
    19621 ctgaataatt caaaaactcc ccgctctcgc tatatgacaa cgaggtgatc cgcagcagtg
    19681 gcgtgtgttc cgctaccttc atttgccgcg ccagcagtct atcggcgagc accggcgtca
    19741 ggctttcata attaccgcca atcagaatcc cgcactcttg ttcaatatat tcaaacttcg
    19801 acccttccag atgttgcagc gaaaggttac ggaacagttt taccggcata tagctgtctt
    19861 ccagcatcag cggtttccct tccacaaaac gaacgcgacg ggagaagtag atctgctcgt
    19921 tgatttgaat ccgtaactgg ctggcaatgg ccggaggcgc aggcatgatt tcaaaaatca
    19981 gcacctggct ggtgaccgtt tttccctgac gttttaacac ctccaccagc ccggtcagac
    20041 tggcggtttg atgcagtaca tctttgcgca ccaggtaagt gccactaccg tggcggcgga
    20101 ccaccagccc ccacgctacc agcaggtcaa tggctttacg gatggtcatc cgcgataccg
    20161 caaactcttc cgccagcttt ttttcaccgg gaagaaggct gtttagcgtg aaatctgcgg
    20221 aattaagtcg aattcttaac cgctccgcaa tgcttttgta gatcatctct agatctcttt
    20281 tttgcctgca agcccgggta atgcgcccac aagttctgct taatcgattg aaaaaacatc
    20341 ttttttttaa agatgtgttc gatgctgtgt accagtgctc acagaggtct actttttcgc
    20401 gaaaacgtag atctctaacg cccaacgaaa agcatgaaag cgatcacgaa tcccattggg
    20461 tcgtcatgtt ctgccgatcg catcttccta tcctcgctcc aggcctgccg cataaccaat
    20521 caggcttcct acttacagaa ttgagaaaag aggatgtgga aatgctcagt caaattcaac
    20581 gctttggcgg cgcgatgttc acgccagtgc tgctgtttcc cttcgccggg attgtggtgg
    20641 gtcttgctat cttgctgcaa aacccgatgt ttgtcgggga atcactgacc gatccgaaca
    20701 gtttattcgc gcaaatcgta cacattattg aagagggcgg ttggacggta ttccgtaata
    20761 tgccgctgat ttttgctgtc ggtttaccca ttggccttgc taagcaagcg caggggcgtg
    20821 cttgtctggc ggtgatggtg agtttcctga cctggaacta tttcatcaat gcgatgggaa
    20881 tgacctgggg aagctacttc ggtgtcgatt tcactcagga cgcggtggca ggtagcggtc
    20941 tgacaatgat ggccgggatt aaaaccctcg ataccagcat tatcggcgca attatcattt
    21001 ccggcattgt gacggcgctg cataaccgtc tgttcgataa aaaactgccg gtgtttctcg
    21061 gcattttcca ggggacgtct tatgtggtga ttatcgcctt cctggtgatg atcccctgtg
    21121 cctggctgac attgctcggc tggccaaaag tacaaatggg gattgaatct ctgcaagcgt
    21181 tcctgcgttc ggcgggtgcg cttggggtgt gggtttacac cttcctcgaa cgtattctga
    21241 tcccaaccgg tttacatcac ttcatctacg gaccgtttat ctttggtccg gcagctgttg
    21301 aaggcggcat tcagatgtac tgggcgcagc atctgcaaga gttcagtctg agcgccgagc
    21361 cgctgaaatc gttgttcccg gaaggcggtt ttgccctgca cggtaactca aaaatctttg
    21421 gtgccgtggg catttcttta gcgatgtact tcactgccgc accggaaaat cgggtaaaag
    21481 tggcgggctt gctgattccc gcaaccttaa ccgccatgct ggtgggaatt accgaaccgc
    21541 tggaatttac cttcctgttc atttcaccgt tgctgtttgc ggtacacgct gtgcttgcgg
    21601 cctcaatgtc gaccgtgatg tatctctttg gtgtggtggg caacatgggc ggaggtctga
    21661 ttgaccaggt tttaccgcaa aactggatcc cgatgttcag caaccacgcg gatatgatgc
    21721 tgacccaaat cgccattggg ttgtgcttta ccctgctgta cttcgtggtt ttccgcaccc
    21781 tgattctgca attcaacatg tgcacgccgg gacgtgaaga tgcggaagtg aaactctact
    21841 caaaagccga atacaaagcc tcgcgaggcc aaaccaccgc ggcagagcca aaaaaagagc
    21901 tggatcaggc tgccggtatc ctgcaagccc tgggcggggt cggcaatatc tccagcatca
    21961 acaattgcgc gacgcgtcta cgtattgcac tgcatgacat gtcacaaacg ctggatgacg
    22021 aagtctttaa aaagctggga gcgcacggcg tcttccgtag tggcgatgcc attcaggtga
    22081 tcattggtct gcatgtatcc cagctgcgtg aacagctcga tagcttaatt aattctcatc
    22141 aatcaacaga aaatgttgcc attacggagg cagtataatg accaaattct cagtggttgt
    22201 cgcaggcggt ggaagcacct ttacgccagg catcgtgttg atgctcctgg cgaatcagga
    22261 ccgtttcccg cttcgtgcac tgaaatttta tgataacgat ggtgcgcggc aggaagtgat
    22321 tgccgaagcc tgtaaagtca tccttaaaga aaaagcgccg gatattgcgt ttagttacac
    22381 caccgatcct gaagtggcat tcagcgacgt tgattttgtc atggcgcaca tccgcgtcgg
    22441 caaatacccg atgcgcgaac tggatgaaaa aatcccgctg cgccacggcg ttgttggtca
    22501 ggaaacttgc ggacccggcg gaatagcgta cggcatgcgt tccattggcg gcgtcctgga
    22561 actggtggat tatatggaaa aatattcacc aaacgcctgg atgctcaact actccaaccc
    22621 ggcagccatt gtcgcagaag ccacgcgtcg tctgcgtccg aatgcgaaaa tcctcaacat
    22681 ctgtgacatg ccaatcggta ttgaaagccg gatggcgcaa attgttgggc tgcaagatcg
    22741 caaacagatg cgcgtgcgct actacggcct gaaccacttt ggctggtgga catcaattga
    22801 agatttgcag ggcaacgacc tgatgcccca gctgcggcaa tatgtctcta agcacggtta
    22861 tgttccaccg cagcaagata cacatactga agcgagctgg aacgacacct atgcaaaagc
    22921 gcgggatgtc caggcactgg acccggatac attgccgaac acctatctga aatattatca
    22981 cttcccggat tatgtcgttc agcattccaa ccctgaacat acccgcgcga atgaggtgat
    23041 ggaacatcgc gagaaacagg ttttcgatgc ttgccgcgcc attacggcgg caggaaattc
    23101 agcggcgggc aagctggaga ttgacgaaca tgcgtcatac atcgtcgatc tggcgacggc
    23161 aattgccttc aacactcagg agcggatgtt gctgattgtg cctaacaacg gggcaatcca
    23221 taactttgat gatgaagcga tggtcgagat cccgtgtctg gtcgggcaca acggaccaga
    23281 accactggtg gtcggcgata tcccgcagtt tcagaaaggg ttaatgagtc agcaagtggc
    23341 ggtggaaaaa ctggtcgtgg acgcctggga acagcgttca tatcagcacc tgtggcaggc
    23401 gattacgttg tcgaaaacgg taccgagcgc ctcggtcgcc aaagctattc tggatgagtt
    23461 gctggaggcc aacaaagcgt actggccaga gttacgttaa ttgtgtggac tggcatccgt
    23521 tgggtgctgg tccgtattct ttagcacggc tgacggcgca taaccaaatt tatgtttaaa
    23581 ggctttgctg aaatgaaagg agtcgaagaa gttgagcgta tcagcgactt cgccaaccga
    23641 gtttccttgt tgctttaata gcgatagcgc cagatccaga cgcgcatcga ggtaatattc
    23701 tttcggcgtc ttgccggtat agcgtaaaaa cagacgacgc aaccaggctt cgctacaggg
    23761 gatcgtggca gccatatcag ctacgctcca gcgttgttgc agactggcat gtaaagtggc
    23821 aattaatttt tcaatttgcc gccgttgtgg atctttttta ccatctgcgt acataagaca
    23881 aatccactga taaataattt tcgtcagaaa agcgactgcc agattatttt ttattgcttc
    23941 tggtgaagtt attaactcag caacttcggt gagttcctga ttataaattt cgccattata
    24001 aataacgctt tgctgaccaa cgggaatatc catcatactg gtgggggtaa attccatcca
    24061 gtactgttcc cagactaaac cttcacagtg ataagagtga atatccattg gctttaagaa
    24121 tataatacag ttgccatgca gtgttatttg ttcgccattc tttaaaagaa ttttcccaca
    24181 tccttgtacg gtatacaccg caacccatga gttggcgata agcggtttct tcttgtcccg
    24241 cggccagatc acccggtaat gctcgtcggc gtaggtatgc cagagcgaga tcagagaaat
    24301 tccgctggag atcacatttt cgttaatcaa taagggaata ttgtatggag tttcgttttt
    24361 tacatctgaa gtttgcaatt ttccattcat tttaatatcc ctccggcgaa ttatgctcac
    24421 aacctgaact taacagaaaa taatccgttc agggatacga cgtttgtatt ttaagaatct
    24481 gactgcctga cccgacgcat tttacctctc cctatattca tgcgtccggg acataaacat
    24541 aaataagggc tatgagatga attcgttaca aatcttgagt tttgtcggtt ttacgctgct
    24601 ggtggcgatc atcacctggt ggaaggttcg caaaacagat accggatcgc aacaaggcta
    24661 ttttcttgcc ggacgttcac taaaagcgcc ggttattgcc gcttcgttaa tgttaacgaa
    24721 tctttccacg gaacaactgg ttggtctttc cgggcaggcc tacaaaagcg gcatgtcggt
    24781 gatgggctgg gaagtcactt cagcggtgac gctgatcttc ctcgcgctaa tctttttacc
    24841 gcgctatctg aagcgcggca ttgccaccat ccccgatttt ctggaggaac gttatgataa
    24901 aacgacgcgt attatcatcg acttctgctt cctcattgcc accggcgtct gctttctgcc
    24961 gattgttctc tactccggcg cgttggcgct caacagcctg tttcacgtcg gggaatcgct
    25021 acagatttct cacggtgcgg ctatctggct attggtaatt ttgcttggtc tggcgggaat
    25081 tttgtatgcg gtgatcggcg gactgcgcgc aatggcagtg gcggactcca tcaacggtat
    25141 tgggctggtt atcggcgggt tgatggtgcc ggtatttggc ctgatcgcga tgggcaaggg
    25201 cagctttatg cagggcattg agcaaattac caccgttcac gccgagaaat taaactcagt
    25261 cggtggcccg accgatccct tgccgattgg cgcggcattt accggtttaa ttctggtgaa
    25321 caccttttac tggtgtacaa atcagggcat cgtgcaacgc acgctggcgt caaaaagcct
    25381 ggcggaaggg caaaaggggg cgctgttaac ggcggtgctg aaaatgctcg acccgctggt
    25441 actggtgctg ccagggttga ttgcgtttca tttgtatcag gatttaccga aagccgacat
    25501 ggcctacccg acgctggtca ataacgttct gccagtgcca ctggtgggtt tcttcggcgc
    25561 ggtgttattt ggtgcggtga tcagtacctt caacggcttt ctgaatagcg ccagtacgtt
    25621 attcagtatg ggtatttacc gtcgcatcat taaccagaat gccgagccgc agcagctggt
    25681 caccgtcggg cgcaaatttg gtttctttat cgctatcgct tcggtgctgg tagcgccgtg
    25741 gatcgccaac gcgccgcagg ggctgtatag ctggatgaaa cagctcaacg gcatttacaa
    25801 cgtgccgctg gttaccatca tcattatggg ctttttcttc ccgcgcatcc cggcgctggc
    25861 ggcaaaagta gcgatgggga ttggcataat cagctacatc accatcaact atctggtgaa
    25921 gttcgacttc catttcctct atgtgctggc ctgtacgttc tgcatcaacg tggtcgtgat
    25981 gctggtgatc ggttttatca aaccgcgcgc cacgccgttc accttcaaag atgcgtttgc
    26041 ggtggacatg aaaccgtgga aaaacgtcaa gatcgcgtca attggcatcc tgttcgcgat
    26101 gattggcgtc tatgccgggc tggctgaatt cggcggctac ggtacgcgct ggttagcgat
    26161 gatcagttat ttcattgccg ccgtagtgat tgtctacctg atttttgaca gctggcggca
    26221 tcgtcacgac ccagccgtaa cctttactcc cgatgcaaag gatagcctat gaaacgcccc
    26281 aattttctgt tcatcatgac cgatacccag gccaccaata tggtcggttg ctatagcggt
    26341 aagccgctga atacgcaaaa tattgatagt ctggcggcgg aaggtattcg ctttaactcc
    26401 gcctacacct gttcaccggt ttgtacaccg gcgcgcgccg ggctgtttac cggtatctac
    26461 gctaaccagt ccggcccgtg gaccaataac gtcgcgccag gcaaaaacat ttccactatg
    26521 gggcgctact ttaaggatgt cggctatcac acctgttaca tcggcaaatg gcatctcgac
    26581 ggtcatgact atttcggcac tggcgagtgt ccgccggagt gggacgctga ttactggttc
    26641 gatggagcga actatcttag cgaactgacg gaaaaagaga ttagcctgtg gcgcaatggc
    26701 ctaaacagcg tcgaagattt acaggcgaac catatcgacg aaaccttcac ctgggcgcac
    26761 cgcatcagca atcgggcggt ggattttctg caacagcccg cgcgcaccga ggaacccttc
    26821 ctgatggtgg tttcgtatga tgagccgcat cacccgttca cctgtccggt ggagtattta
    26881 gagaaatacg ctgattttta ctacgagctg ggcgagaaag cacaggatga cctggctaac
    26941 aaaccggaac atcaccgctt atgggcgcag gcgatgccat cgccagtcgg tgatgacggg
    27001 ctttatcacc atccgctcta ttttgcctgt aatgactttg ttgatgacca aatcggacgg
    27061 gtcatcaatg ccttaacgcc agagcaacgt gaaaatacgt gggttattta tacctccgat
    27121 cacggcgaaa tgatgggcgc acataagctg atcagtaaag gggcggcgat gtatgacgac
    27181 atcacccgca ttccgctgat catccgttcg ccgcaagggg agcggcgaca ggtcgatacg
    27241 ccagtcagtc atatcgattt actgccgaca atgatggcgc tggcagatat tgaaaaacca
    27301 gagattctgc cgggggaaaa tatccttgca gtgaaagagc cacgcggtgt aatggtggaa
    27361 tttaaccgct acgagattga gcatgacagc tttggcggtt ttattccggt gcgttgctgg
    27421 gtgacggatg actttaaact ggtgctcaac ctcttcacca gtgatgaact ttacgatcgc
    27481 cgtaatgatc caaatgaaat gcataacctg atcgatgata tccgttttgc cgacgtgcgc
    27541 agcaaaatgc atgacgcctt attggattac atggacaaaa ttcgcgatcc gttccgcagt
    27601 taccaatgga gcctgcgtcc gtggcgtaaa gatgcacggc cgcgctggat gggggcgttt
    27661 cgtccacgcc cacaagatgg ctattcgcca gtggtacgcg actatgacac cggcctaccg
    27721 acacaagggg tgaaggtgga agagaaaaaa cagaagttct gacgataatg cccgctgatg
    27781 atcacccggc gggcattatt caggctgctt aaaccagtga accgccggtc aggaaggaaa
    27841 agataaggaa tatcagcaaa atgctgcaca cgatgccgat accaaaaagc aaggtatgga
    27901 aagccagcgt cccgccgcca aaaaccagcg cgccgcgaat gccccagcga ttttccttac
    27961 gcataccgcc aataaatgcg caaagtagcg cagcaatggc cagtgcgata ccgctttgat
    28021 tgaggatttt atcgacatct acgtcctctt ttttctccac ggtggtaatt ttttcgcctt
    28081 ttattccggc gacaagacct cttttaattt cagccgtttt gtccgccacg atgctttcaa
    28141 acgttggcgg tggtgcggag aaagggccga atgaaaagtg gataatcccc atcattaaag
    28201 cgatggcacc gaacagcatg cccaatgaac tgattttatt ttgagctatg atacccatct
    28261 gacctccttg tagcggatgg caaaagcata tcaggaaacg ggcggacgaa attcttaaaa
    28321 aaaaccgcta aaggtagagc gttagtgaaa ttgcaaagga gcaacaatga aaatttcccg
    28381 cctcggagaa gcgcccgatt accgcttctc gctggctaat gagcgtactt ttctggcgtg
    28441 gatccgtact gcactaggat ttctggcggc gggggttggg cttgatcaac ttgcgccaga
    28501 tttcgccacg cccgtcattc gtgaactgtt ggcgttgttg ttgtgtctgt tttccggcgg
    28561 gttagcgatg tatggctacc tgcgctggtt acgcaatgaa aaagcgatgc gtctgaaaga
    28621 ggacctgcct tacactaaca gcttattaat catcagctta atattgatgg tcgtggcggt
    28681 gatcgttatg ggactggtgt tgtatgccgg atagccgcaa agccagacgc attgccgacc
    28741 caggactgca gccggagcgt acatcactgg cgtggtttcg taccatgctg ggctacggcg
    28801 cgttgatggc gttggctatc aaacacaact ggcaccaggc gggcatgtta ttctggattt
    28861 cgattggcat cctcgccatc gtggcgctga tcctctggca ctacacccgt aatcgcaatt
    28921 taatggatgt cacgaatagc gatttttccc aatttcacgt agtccgtgac aaatttttga
    28981 tctccctcgc ggtgttatct ctcgcaatac tgtttgctgt aacgcatata catcaactta
    29041 tcgtatttat tgagagagtc gcatgacagg aagtcaggtc attgatgctg aagaggacag
    29101 gcataaactg gttgtagagt ataaggatac tctgcaacct gccgattttt atcacaattt
    29161 caaacagcgg ggcatccgct ccgtacagct tattccccat cttgaatttg atgaccgggg
    29221 cgatatgacg gccgcttcgg tgacagcaga gctgtggggg aaatttttaa ttgccctgtt
    29281 tgagtgttgg gtgcgggcag atatcagtcg tatttcgatt gagctttttg atgccacttt
    29341 acaaaaatgg tgtggcagcg aaaacccgca cccgcgccgt gactgccagg cgtgcgactg
    29401 gcatcggctg tgtccgcacg ctcgggagga gacgccagac aacgtgcttt gtgcgggcta
    29461 tcaggcgttt tattcttact ccgcaccgca tatgcgggtg atgcgtgatc tgataaagca
    29521 gcatcgttct cccatggagc tgatgacgat gctgcggtga tgtgcgctta aacgggctgc
    29581 ccctgatgcg acatccgcgt cgccagcggt agccagcaca gcacgatcaa caatcccatt
    29641 aaggtcatca acaaccccag gctgccctga ccggtttgcg gcaacatcgc tgatagcgac
    29701 gccagcacgc cggaaccaat gttttgcaga ccaccgacca gcgcgccagc cgtgcccgcc
    29761 aggaagggga acggctccat cgcgccgctg gtcgccagcg gaaacagcat cccggcaccg
    29821 aaaaagaaca gcgcggcggg aacaagcagc gtccagacat tcatcacgcc aaaccagtcg
    29881 gggatccaca tcagcaagcc agccagcagg cagcagataa ccgactgcca catcaacgtt
    29941 gagaagcgtt tattaggacg cccggcaaac catgccccaa aaaatgccgc cggaatcggc
    30001 agaataaaca aaatactgac cgtcatactg ctcagcccta acaccgcgcc catcagcacg
    30061 ccggagcagg cttcaaaggc ggcaatcccg gccagaccgc caatcagcat cagcaaataa
    30121 cagttaaaac cgccgttacc gaaaagcgtt ttataactgg taagcaggcg cgtgcgcggt
    30181 gcatcgaccg gacgcgtttc cggcatccag cgggccatac tgaaggtcac accagcacaa
    30241 agaaccaaca aaaagagata acaggcgcgc cagttccaca tcgtatccag cagaccgccg
    30301 attagcggtg cgagcaacgg actaacaaga atccccatgt ttaacaggct gttagcatgg
    30361 cgcaactgtg tccgttcata taaatcacgc ggcaaagtac gcgccattac gccgccaacg
    30421 ccggtgccca tcccctgcat cgcgctggcg gcaattaaca ccgtcaaact ggaggtcgtg
    30481 accgcgacca gcgttgccag cataaaaatg gacattccga tgaggatcac cggtcggcgg
    30541 cccacgcggt cggaaatcgg gccataaaac agctgtgaga caccgtaagt cagcagataa
    30601 gcgcccatta cgctctgcac cgccccttca cggacgttga gatcgcgcgc catatcggca
    30661 atagctggaa tataaatggt ttgcgccatc tgaccgacgg ccacgagtaa taccaacatc
    30721 aataacaaat tgacgtttct ttgccttttc attatcacgg atgcttttat aaaaataatt
    30781 agaataagtc actacgcatt cccataaatg cgggagagga atctatcaca ttgataaatg
    30841 ttagccagat atacgccagg aatggggaat tgtttagcgg cagaatatgt aaacaaaagc
    30901 ggcaataaat gttgccggga gggattacgt gcgaactgta accctctccc tgaaagggcg
    30961 agggggaaac caccgttccc tttcagaaaa tgagctaaac attgcacggg acggtcccct
    31021 cgcccctttg gggagagggc tagggtgagg ggcacaggca gcgcaggtgc aaacattctg
    31081 ctggtagaag aagcccccaa ctccaacccg cacgctaaat ataaaagggg agcggttacc
    31141 cgctcccctt tggtgcgact tgaatctgaa ttacttcagg tatttcagaa cagcatcaag
    31201 cagttgcagt gctgcaacaa tgagtttgag gataagaatg gcgatatcca ccaggctcat
    31261 acgcgtctcc tgtggttcag gagcactgcc agctgacgta cctttccgct gcctgttgcc
    31321 agctgttgtg cacgcggtat ctcaaggaga aacgttgtgc ttgttgacat aacacagtgt
    31381 gctcacgcgg ggttaaggcg ctgacggcac cacccgtttc agccaggact tagtgcgccg
    31441 ggtaacgata cggcccccgc ataacacatt gcgtacagtg atattataga agtttactgt
    31501 ataaataaac agtaatattt agacaaaacg taaactgtga tcgatactca actatgtact
    31561 aaatacgtca aaattcgtgc cgaaattgcg cgttctgcgc ggaacaggta tactttcagt
    31621 gttgacataa tacagtgtgc tttgcggtta ccagccgcag gcggctgacg aaacctcgct
    31681 ccggcggggt tttttgttac ctgcaactca gtacaaaacg tgatcaaccc ctcaattttc
    31741 ccttgctgaa aaattttcca ttgtctcctc tgtaaagctg tgcttgtata aatattgtta
    31801 aacacaaaac caacaaggtc cccaatgact acttccatgt tcaacgcaaa actactacca
    31861 actgcgccat ccgccgcagt ggtcgtcgtg cgtgtggtgg tggtcgtcgg caatgcgccg
    31921 tagggactgg aacaacacac gattccaaaa ccccgccggc gcaaaccggg cggggttttt
    31981 cgtttaagca cctcccggaa agtcggccca gaagaaaagg actggagcat ggcaagttcg
    32041 ggcacatcat cgacgcgtaa gcgctttacc ggcgcagaat ttatcgttca tttcctggaa
    32101 cagcagggca ttaagattgt gacgggcatt ccgggcggtt ctatcctgcc tgtttacgat
    32161 gccttaagcc aaagcacgca aatccgccat attctggctc gccatgaaca gggcgcgggg
    32221 tttatcgctc agggaatggc gcgcaccgac ggtaaaccgg cggtctgtat ggcctgtagc
    32281 ggaccgggtg cgactaacct ggtgaccgcc attgccgatg cgcggctgga ttccatcccg
    32341 ctgatttgca tcactggtca ggttcccgcc tcgatgatcg gtaccgacgc cttccaggaa
    32401 gtcgatacct acggcatctc tatccccatc accaaacaca actatctggt cagacatatc
    32461 gaagaactcc cgcaggtcat gagcgatgcc ttccgcattg cgcaatcagg ccgccctggc
    32521 ccggtgtgga tagacattcc taaggatgta caaacggcag tttttgagat tgaagctcag
    32581 cccgcggtgg cagaaaaagc cgctgcaccc gcctttagcg aagaaagcat tcgtgacgcg
    32641 gctgcgatga ttaacgccgc caaacgcccg gtgctttatc tgggtggtgg tgtaatcaac
    32701 gcacctgcgc gggtgcgtga actggcggag aaagcgcaac tgcctactac catgacctta
    32761 atggcgctgg gtatgctgcc aaaagcgcat ccgctgtcgc tgggtatgct ggggatgcac
    32821 ggcgtgcgca gcaccaacta tattttgcag gaggcggatt tgttgattgt gctcggtgcg
    32881 cgttttgatg accgggcgat tggcaaaacc gagcagttct gcccgaatgc caaaattatt
    32941 catgtcgata tcgaccgcgc agagctgggt aaaatcaagc aaccgcatgt ggcgattcag
    33001 gcggatgttg atgacgtgct ggcgcagttg atcccgctgg tggaagcgca accgcgtgca
    33061 gagtggcacc agttggtggc ggatttgcag cgcgaatttc cgtgtccaat cccgaaagcg
    33121 tgcgatccgt taagccatta cggcctgatc aacgccgttg ccgcctgtgt cgatgacaat
    33181 gcaattatca ccaccgacgt aggtcagcat cagatgtgga ccgcgcaggc ttatccgctc
    33241 aatcgcccac gccagtggct gacctccggt gggctgggca cgatgggctt tggcctgcct
    33301 gcggcgattg gcgcagcgct ggcgaacccg gatcgcaaag tgttgtgttt ctccggcgac
    33361 ggcagcctga tgatgaatat tcaggagatg gcgaccgcca gtgaaaatca gctggatgtc
    33421 aaaatcattc tgatgaacaa cgaagcgctg gggctggtgc atcagcaaca gagtctgttc
    33481 tacaagcagg gcgtttttgc cgctacctat ccgggcaaaa tcaactttat gcagattgcc
    33541 gccggattcg gcctcgaaac ctgtgatttg aataacgaag ccgatccgca ggctgcattg
    33601 caagaaatca tcaatcgccc tggtccggcg ctgatccatg tgcgtattga tgccgaagaa
    33661 aaagtgtacc cgatggtgcc gccaggtgcg gcgaatactg aaatggtggg ggaataagcc
    33721 atgcaaaaca caactcatga caacgtgatt ctggaactca ccgttcgcaa ccatccgggc
    33781 gtaatgaccc acgtttgtgg cctttttgcc cgccgagctt ttaacgttga aggcattctc
    33841 tgcctgccga ttcaggacag cgacaaaagc cacatctggc tactggtcaa tgacgaccag
    33901 cgtctggagc agatgataag ccagatagac aagctggaag atgtggtgaa agtgcagcgt
    33961 aatcagtccg atccgacgat gtttaacaag attgcggtgt tttttcagta aggagcgaca
    34021 ttaaaccgct cggggggttt actgatgagc gatttttgat agatccgttc agatatatag
    34081 ggtgaaattg tgccagagac catctcaggc acaatttatt agccctgaac gttaatttaa
    34141 gtcgctgcac cagtaatttg ggggttaaca gttagggcta aaatttccag cgatggtgtt
    34201 aaattctgat tgctgtctat accaatggtc atgaccagca ttctattaat taatggttca
    34261 tcgtgaggta tattatggca agccctataa acagcggaac aatgatgtca aatcccagcc
    34321 cttccacctt tttggataaa aatagaatta ttgctgctga gcttaatatc acaaataacg
    34381 agaaaaaata ttcacctggc agtaatttcg caaaatggat gttgcaggaa ataaagcgct
    34441 taatagcaaa tattatgtcc ggttctcgca gtgttaatac cgaaattctt aactatttcc
    34501 accctatgcc aggcacggaa aataatggta atcgtacctg gatggcggca actggagaga
    34561 acgaatatac cgtaattgaa caaacaggtg acaaatcatt
//
LOCUS       DAPGOF010000033        34561 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.033, whole genome shotgun sequence.
ACCESSION   DAPGOF010000033 DAPGOF010000000
VERSION     DAPGOF010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.033"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            186..338
                     /gene="mokC"
                     /locus_tag="RHV13_001384"
     CDS             186..338
                     /gene="mokC"
                     /locus_tag="RHV13_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HDV1240937.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(442..1572)
                     /gene="dnaJ"
                     /locus_tag="RHV13_001385"
     CDS             complement(442..1572)
                     /gene="dnaJ"
                     /locus_tag="RHV13_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDV1240938.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1661..3577)
                     /gene="dnaK"
                     /locus_tag="RHV13_001386"
     CDS             complement(1661..3577)
                     /gene="dnaK"
                     /locus_tag="RHV13_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDV1240939.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            3954..4358
                     /locus_tag="RHV13_001387"
     CDS             3954..4358
                     /locus_tag="RHV13_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HDV1240940.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLFADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4384..5097
                     /gene="msyB"
                     /locus_tag="RHV13_001388"
     CDS             4384..5097
                     /gene="msyB"
                     /locus_tag="RHV13_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HDV1240941.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            5246..5812
                     /gene="satP"
                     /locus_tag="RHV13_001389"
     CDS             5246..5812
                     /gene="satP"
                     /locus_tag="RHV13_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HDV1240942.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5847..6434)
                     /gene="mog"
                     /locus_tag="RHV13_001390"
     CDS             complement(5847..6434)
                     /gene="mog"
                     /locus_tag="RHV13_001390"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HDV1240943.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARREVSE"
     gene            complement(6549..7502)
                     /gene="tal"
                     /locus_tag="RHV13_001391"
     CDS             complement(6549..7502)
                     /gene="tal"
                     /locus_tag="RHV13_001391"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDV1240944.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            7781..9211
                     /locus_tag="RHV13_001392"
     CDS             7781..9211
                     /locus_tag="RHV13_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HDV1240945.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVINIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSRDAWDDVSQE"
     gene            9281..10057
                     /gene="yaaA"
                     /locus_tag="RHV13_001393"
     CDS             9281..10057
                     /gene="yaaA"
                     /locus_tag="RHV13_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HDV1240946.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(10210..10506)
                     /locus_tag="RHV13_001394"
     CDS             complement(10210..10506)
                     /locus_tag="RHV13_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HDV1240947.1"
                     /translation="MKKMQSIVLALSLVLVAPMATQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAHHDHHGGHGPGKHHR"
     gene            complement(10720..12006)
                     /gene="thrC"
                     /locus_tag="RHV13_001395"
     CDS             complement(10720..12006)
                     /gene="thrC"
                     /locus_tag="RHV13_001395"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HDV1240948.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(12007..12939)
                     /gene="thrB"
                     /locus_tag="RHV13_001396"
     CDS             complement(12007..12939)
                     /gene="thrB"
                     /locus_tag="RHV13_001396"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HDV1240949.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCHELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(12941..15403)
                     /gene="thrA"
                     /locus_tag="RHV13_001397"
     CDS             complement(12941..15403)
                     /gene="thrA"
                     /locus_tag="RHV13_001397"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HDV1240950.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQIWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15429..15548)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15484..15549)
                     /gene="thrL"
                     /locus_tag="RHV13_001398"
     CDS             complement(15484..15549)
                     /gene="thrL"
                     /locus_tag="RHV13_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HDV1240951.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(15763..16449)
                     /locus_tag="RHV13_001399"
     CDS             complement(15763..16449)
                     /locus_tag="RHV13_001399"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HDV1240952.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAYLEPAT
                     RWVAHGSGDIIDNIKVFPTLVESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPTKSDTTADQHQLQALRERAMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(16849..16989)
                     /locus_tag="RHV13_001400"
     CDS             complement(16849..16989)
                     /locus_tag="RHV13_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1240953.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            17085..17801
                     /gene="arcA"
                     /locus_tag="RHV13_001401"
     CDS             17085..17801
                     /gene="arcA"
                     /locus_tag="RHV13_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HDV1240954.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(17861..19213)
                     /gene="creD"
                     /locus_tag="RHV13_001402"
     CDS             complement(17861..19213)
                     /gene="creD"
                     /locus_tag="RHV13_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HDV1240955.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPTGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKDVTTDDELRIWK
                     "
     gene            complement(19271..20695)
                     /gene="creC"
                     /locus_tag="RHV13_001403"
     CDS             complement(19271..20695)
                     /gene="creC"
                     /locus_tag="RHV13_001403"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HDV1240956.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPESGCITLS
                     AEVDHEHVTLKVLDTGSGIPYYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(20695..21384)
                     /gene="creB"
                     /locus_tag="RHV13_001404"
     CDS             complement(20695..21384)
                     /gene="creB"
                     /locus_tag="RHV13_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HDV1240957.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QLVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(21397..21870)
                     /gene="creA"
                     /locus_tag="RHV13_001405"
     CDS             complement(21397..21870)
                     /gene="creA"
                     /locus_tag="RHV13_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HDV1240958.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            22081..22950
                     /gene="robA"
                     /locus_tag="RHV13_001406"
     CDS             22081..22950
                     /gene="robA"
                     /locus_tag="RHV13_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HDV1240959.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(22947..23594)
                     /gene="gpmB"
                     /locus_tag="RHV13_001407"
     CDS             complement(22947..23594)
                     /gene="gpmB"
                     /locus_tag="RHV13_001407"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HDV1240960.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRINAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            23646..24158
                     /gene="yjjX"
                     /locus_tag="RHV13_001408"
     CDS             23646..24158
                     /gene="yjjX"
                     /locus_tag="RHV13_001408"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HDV1240961.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVY"
     gene            complement(24305..24631)
                     /gene="trpR"
                     /locus_tag="RHV13_001409"
     CDS             complement(24305..24631)
                     /gene="trpR"
                     /locus_tag="RHV13_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HDV1240962.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(24721..26658)
                     /gene="sltY"
                     /locus_tag="RHV13_001410"
     CDS             complement(24721..26658)
                     /gene="sltY"
                     /locus_tag="RHV13_001410"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HDV1240963.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTEGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            26869..28536
                     /gene="ettA"
                     /locus_tag="RHV13_001411"
     CDS             26869..28536
                     /gene="ettA"
                     /locus_tag="RHV13_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HDV1240964.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(28643..28810)
                     /locus_tag="RHV13_001412"
     CDS             complement(28643..28810)
                     /locus_tag="RHV13_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1240965.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(28844..30076)
                     /gene="nadR"
                     /locus_tag="RHV13_001413"
     CDS             complement(28844..30076)
                     /gene="nadR"
                     /locus_tag="RHV13_001413"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HDV1240966.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEEDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(30097..31479)
                     /gene="radA"
                     /locus_tag="RHV13_001414"
     CDS             complement(30097..31479)
                     /gene="radA"
                     /locus_tag="RHV13_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDV1240967.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(31528..32496)
                     /gene="serB"
                     /locus_tag="RHV13_001415"
     CDS             complement(31528..32496)
                     /gene="serB"
                     /locus_tag="RHV13_001415"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HDV1240968.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKTEVTIRHADLMGVFCILSGSLNQK"
     gene            32602..33246
                     /locus_tag="RHV13_001416"
     CDS             32602..33246
                     /locus_tag="RHV13_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HDV1240969.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVDVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            33274..34290
                     /gene="lplA"
                     /locus_tag="RHV13_001417"
     CDS             33274..34290
                     /gene="lplA"
                     /locus_tag="RHV13_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HDV1240970.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITEAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            34322..34471
                     /locus_tag="RHV13_001418"
     CDS             34322..34471
                     /locus_tag="RHV13_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1240971.1"
                     /translation="MIDLENQEREIINLMLSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLK"
BASE COUNT         8136 a         9401 c         8633 g         8391 t
ORIGIN      
        1 acagaatggg ttaaagagag cgatataata gcggcgggtg cttgaggctg tctgtctcag
       61 gcattagctg aacggcagat agagaaaagc cccgagtgat attttaccat caacccgagg
      121 ccccctatat gcagaacaca tgtagagtgc ctcttactga ccgtaaggtc aaggagaaga
      181 gagcaatgaa gcagcataag gcgatgattg tcgccctgat cgtcatctgt atcaccgccg
      241 tagtggcggc gctggtaacg agaaaagacc tctgtgaggt tcacatccga actggccaga
      301 cggaggttgc tgttttcacg gcttacgaat ccgagtaaga gcaacggcgg ggagtgatcc
      361 ccgccacttt acaggtgctc gcatatcttc aacgcaccct atttttaccc aggcctgccc
      421 acgggcaggc ttttggggag gttagcgggt caggtcgtca aaaaacttct tcacaccatc
      481 aaagaagctc tttgagcgcg ggctgttgtg ctcgccggtt gggccaccga agctttcttg
      541 cagctcttgc agcagctgtt tctgcttctc gttcaggcct accggtgttt cgacgacaac
      601 gcggcacagc aaatcaccct gtgcgccacc gcggacagac ttgacgcctt taccgcgcat
      661 acggaatagc ttaccggtct gggtttcgcc aggcactttc agtttgacgc gaccatcaag
      721 ggtcggtact tcgatttcgc cacccagcgc cgccatagcg aagttgatcg ggacttcgca
      781 atacaggttg ttgccttcac gctcgaaaat cgggtgctgt ttaacctgaa cctgaacgta
      841 cagatcgcct gccggtgcgc catgctcgcc cgcttcacct tcgcccgcaa gacggatgcg
      901 gtctccagtg tccacccctg ccgggatttt aacggacagc gttttgctgc gctcaacacg
      961 accatgacca tgacatttgt tgcacggatc tttgatcagc gtaccgcggc cctgacagtg
     1021 tggacaggtc tgctgcacgg caaagaaacc ctggcgcatc tgcacctgac cagaaccatg
     1081 acaggtcgga caggtctgcg gctgtgtacc tggttttgca ccgctaccgt ggcaaacgtc
     1141 acactcttcc agagtcggaa tgcggatctc tttggtcacg ccacgtacag cttcttcgag
     1201 ggtgagctcc atgttatagc gtaaatcagc accgcgcgcc gcacgttgac gaccacgtcc
     1261 gccgccaaaa atatcgccga aaacgtcacc aaaaatatcg ctgaagtctg cgccgccgcc
     1321 aaaaccgccg ccgcccatgc caccttgctc aaacgcagca tgaccatact gatcgtatgc
     1381 cgcacgtttt tgcgagtcgg tcagaacttc ataagcttcc ttgatctctt taaatttcgc
     1441 ctcggcctct ttgtcaccct ggttacggtc cgggtggtat ttcatggcca ggcgtttgta
     1501 ggcctttttg atttcacgct cttccgctgt tttggaaacg cctaaaatct cgtaataatc
     1561 ttgcttagcc atctttttta aattgcccct agatgaatgc acgggcggag aggaaattcc
     1621 ccttcgcccg tgtcagtata attacccgtt tatagggcga ttattttttg tctttgactt
     1681 cttcaaattc agcgtcgaca acatcgtcat ctttcgcgtt gtttgcagaa gcatcagcac
     1741 cggcagtctg ctgctgggca tgttgctgct gggcgatttc catcagtttc tgggaaacct
     1801 gtgccagttc ctgcattttc gcttcgatag cggctttgtc ttcacctttc agagcagttt
     1861 ccagtgcagt cagcgcagac tcgatagcag ttttgtcgtc agccggcagt ttgtcgcctg
     1921 cttcttcaac ctgcttacgg gtgctgtgca gcagatggtc gccctggttg cgagtctgta
     1981 ccagctcttc aaacttacgg tcagcttcgg cgttagcttc tgcgtcgcgt accattttct
     2041 ggatttcatc ttcgttcaga ccagaagacg ccttgatggt gatcttctgc tctttaccgc
     2101 tgtttttatc tttcgcggaa acgtgcagga taccgtcagc atcgatatcg aaggtaactt
     2161 cgatctgcgg catgccgcgc ggtgccgggt tgataccatc caggttgaac tgacccagag
     2221 atttgttatc agccgcacgt ttacgttcac cctgcagcac atggatggtt accgcagact
     2281 ggttgtcttc agcggtagag aacacctggc tgtgcttggt cgggatagtg gtgtttttcg
     2341 cgatcagcgt cgtcatcaca ccgcccatgg tttcgatacc cagagacagc ggggtaacgt
     2401 ccagcagcag tacgtctttt acgtcaccag tcagaacacc accctgaaca gcagcaccga
     2461 ttgctacagc ttcgtccggg ttaacgtctt tacgcggctc tttaccaaag aactcagcaa
     2521 ctttcttctg aaccattggc atacgagtct gaccaccaac gaggataacg tcgtcgatat
     2581 cagatacgga caggccagcg tcctgcagtg caactttcag cggctcaatg gaacggttta
     2641 ccagatcttc aaccaggctt tccagtttcg cacgagtcac tttgatgttc atgtgtttcg
     2701 gaccggtcgc gtctgcagtg atgtacggca ggttaacgtc ggtctgctga gcggaagaca
     2761 gttcgatttt cgctttttct gccgcttctt tcaggcgctg cattgccagc ggatcgttgc
     2821 gcaggtcaat gccctgatct ttcttgaatt cttcaaccag atagttgatc agacggctgt
     2881 cgaagtcttc accacccagg tgggtatcac cgttggttgc cagaacttcg aaggtttttt
     2941 cgccgtcaac ttcgtcgatt tcaataatag aaatatcgaa agtaccacca cccaggtcat
     3001 aaaccgcgat agtacggttg ccagtacctt tgtccagacc gtaagccagc gcagctgcgg
     3061 tcggttcgtt gatgatacgt tttacttcga gaccagcgat acggcctgcg tctttggttg
     3121 cctgacgctg agcatcgtta aagtatgccg gtacggtgat aacagcttca gttaccggtt
     3181 cacccaggta atcttcagcg gttttcttca tttttttcag cacttcagca gaaatctgcg
     3241 gcggtgccat tttctggcct ttaacttcga cccatgcgtc gccgttatca gcagcaataa
     3301 ttttgaacgg catgatggaa acatcacgct gtacttcttc gtcctggaag cggcggccaa
     3361 tcaggcgttt aatcgcaaac agggtgtttt gcgggttcgt cactgcctga cgtttagccg
     3421 gctgaccaac cagagtttca ccatcctggg tataggcaat gatagaaggc gtggtgcgat
     3481 cgccttcggc gttctccagc acacgaggag tggtgccatc cataatcgct acacaagagt
     3541 tggtagtacc caggtcgata ccaattattt tacccatcta aacgtctcca ctatatattc
     3601 ggtcatcatg tggttgtgag tctgtaatag gggcgaaacg tctggtttca actgcccaat
     3661 ttcatttttt tgcagactca ctcactgcgg ttgactacta aatggggtcg taacccacgt
     3721 catcaagggg gagatgcaaa aattttttgt gcttttgcgc agaaatttcg cgatatggca
     3781 ggacaagacg taaaattgac agcaatttta ttcatataaa gaatgaatcg cagtcaaggc
     3841 attcatcaaa aaattgtaat aaaaagaaaa gattacgtgc ctgaatcttc tctttatcag
     3901 cagtaaacta gtgggtattc atccccctac ctcttcccac taagagaatc cttatgaaat
     3961 ccgtttttac gatttccgcc agcctggcga ttagtctgat gctgtgctgc acggcgcagg
     4021 caaacgacca taaactcctc ggcgtcattg caatgccgcg taacgaaacc aacgatctgg
     4081 cgctgaaact tcctgtttgt cgcattgtga aacgcataca actctttgcc gaccatggcg
     4141 atttacagtt aagcggtgca tcggtttatt tcaaagccgc ccgtagcgcc agtcagagcc
     4201 tgaatattcc ttcagaaata aaagaagggc aaaccactga ctggatcaac attaacagcg
     4261 ataacgacaa taaacgctgc gtctcaaaaa tcaccttttc cggtcatacg gtgaactcat
     4321 cggatatggc cacgctgaaa attatcggcg acgactaacg ccagaatata gaagccacaa
     4381 aaaatgaatg ttaattacct gaatgattca gatctggatt ttctccagca ttgtagtgag
     4441 gaacagttag caaatttcgc ccggctgctc actcataatg aaaaaggcaa aactcgcctc
     4501 tccagcgtac tgatgcgcaa cgaactgttt aaatcgatgg aagggcatcc cgagcaacat
     4561 cgccgcaact ggcagctgat tgctggagaa ttacagcatt ttggtggcga tagtatcgcc
     4621 aacaaactgc gcggacacgg taaattgtat cgggccattt tgctcgatgt ttcaaagcga
     4681 ttgaagctga aagccgacaa agagatgtct acgtttgaaa ttgagcaaca gttactggaa
     4741 caatttctgc gtaatacctg gaagaaaatg gacgaggaac ataagcagga gtttctgcac
     4801 gcggtcgatg ccagggtgaa tgagctggaa gagctgctgc cgctactgat gaaagacaaa
     4861 ttattggcaa aaggcgtgtc gcatttgctt tccagccaac tgacccgcat tttacgcacc
     4921 cacgcagcaa tgagcgtgct tgggcatggt ttgctgcgcg gcgcgggtct gggaggccct
     4981 gtgggtgcag cattaaatgg ggttaaagcg gtcagcggca gcgcctatcg cgtgacgatt
     5041 ccagccgtac tgcaaatcgc ctgcctgcgc cggatggtta gcgccactca ggtctgatac
     5101 cggaaaccgg ggaaaatttt cattttcccc tcaaaagatc gtagacactg ccccactggc
     5161 tgattattat gccgcgccct gaaaacacta cagttattca gggaaattat ttcaccattc
     5221 attcgatgat gatttttgag gaattatggg caacactaag ttggctaatc cggcaccgct
     5281 gggcctgatg ggcttcggca tgaccaccat tctgcttaac ctgcacaacg tgggttattt
     5341 cgctctggac ggtattattc ttgccatggg cattttctac ggcggcatcg cgcaaatttt
     5401 tgccggtctg ctggagtaca aaaaaggcaa cactttcggt ttaaccgcat tcacctctta
     5461 cggttctttc tggctgacgc tggttgcgat tctgctgatg ccgaaactgg gtctgaccga
     5521 tgcgccaaat gcacagttcc ttggtgtcta cctgggtctg tggggcgtat ttacgctgtt
     5581 tatgttcttc ggcactctga aaggcgcacg cgtcctgcaa ttcgttttct ttagcctgac
     5641 cgtgctgttt gccctgctgg cgatcggtaa cattgccggt aacgccgcaa tcatccactt
     5701 tgccggctgg attggtctga tctgcggtgc cagcgcaatc tatctggcga tgggtgaagt
     5761 actgaacgag cagtttggtc gcaccgttct gccgattggt gaatcccact aattgttttg
     5821 agatcccccc gctcgggggg atttttttat tcgctaactt cgcgtcttgc actcttcggt
     5881 ctgaatgcag caaccacttc tggtgccgtt tcaacgtatg gcccttccag caactgaatg
     5941 cagtacggta cgctggcaaa aataccgtgt accacaacgt taccctcagc gtccttcaca
     6001 ccttccagcg tctctttaat agacttcggc tgaccaggta agttaaggat cagcgcctgt
     6061 ttgcgaatca cacccacctg acgcgaaagg atcgcagttg gtacaaaatg caggctgatc
     6121 tggcgcatct gttcaccaaa gccaggcatc tcgcggtccg ctactgccag cgtcgcatcg
     6181 ggcgttacgt cacgacgcgc cgggccagtt ccgcccgtgg tgagcaccag atggcaactc
     6241 atttcatcca ccagctcaca caacgtttgc tcgatgatcg cctgctcatc ggggattaag
     6301 cgggtttcca gttcaaacgg cgtggttagc gccgatgtca gccattcttc cagcgcaggg
     6361 atacctttat cctgataaac gccgctggat gcgcgatcag agatggaaac taagccaata
     6421 cgtaaagtat tcatcaagat ttttccgttt aaacagtctc gttaaacaga atgatacact
     6481 gcgaagggag tgacagacag gcttcagaag aggtagcgtg accgacttcc cggtcacgct
     6541 aagaatgatt acagcagatc gccgatcatt ttttccagtt tttcctggtc aatagcaaac
     6601 ttacggatac cttccgccag tttatctact gccattggat cctggttgtg ctgccacagg
     6661 aactcggact cagtgatacg cgccggacgc gctttcactt cgccggtgta agacagttta
     6721 cgttcgatag ccccttcgct ctcagccagc tctttcagca gtgccggtgc gatggtcaga
     6781 cggtcgcagc ctgccagttc cagaatttcg ccgatgttac ggaagcttgc gcccataacc
     6841 acggtttcat aaccgtgctc tttgtagtac tggtagattt cagatacaga aaccacgccc
     6901 ggatcttctg ccggagcgta ctctttctta tcggtattcg ctttgtacca gtcaagaata
     6961 cggccaacaa atggagagat cagaaacacg cccgcttccg cacaagcacg agcctgagcg
     7021 aaggagaaca gcagggtcag gttacagttg ataccttctt tttccagctg ttccgcagca
     7081 cggataccct gccaggtaga agccagtttg atcagaatac gatcgttgct aatacctgca
     7141 tcgttgtaga gtttgatcag gcgttttgct ttcgcaattg acgcttcggt gtcataggaa
     7201 agacgcgcat caacttcagt tgagatacgg cccggaacca gtttcaggat ttccagacca
     7261 atatttactg ccagtttgtc ggtcgcgtcc acgatctgct gcgcgcgatc gttgctctgc
     7321 tgtttcgccc aggcgacagc atcatcaatc aacttacgat attccggaat ctgcgctgcg
     7381 ttaagaatga gagaagggtt ggttgtggca tcctgcggtt gatacagctt cattgccgcg
     7441 atgtccccag tgtcggccac tacggtggtg tactgacgaa gggaggtcaa tttgtccgtc
     7501 atgatagtat ttctctttaa acagcttgtt agggggatgt aaccggtctg ccctgatgat
     7561 atcacgacgc aacgtcagcg caaccgccga caagacggtt atcgccaggc gagactgttt
     7621 cggatttctg acactgcgtt gatatcgccc gccattttta aacaaaacct catgtatgct
     7681 acgcagaagt tatcaagtac ctcgtagcgt atatacttct taaacaattg gtaacgttta
     7741 cacaggaaag tcatcgcgac cggcaataag agggatatgc atgccagatt ttttctcctt
     7801 tattaacagc gtcctttggg gatcggtaat gatttacctg ctcttcggcg caggttgttg
     7861 gttcactttt cgcaccggat ttgtgcagtt tcgctacatc cgccagtttg gcaaaagtct
     7921 taaaaatagc attcatccac agccaggcgg tttaacctca tttcagtcat tgtgtaccag
     7981 tcttgcggcg cgcgtgggta gcggcaacct ggccggcgtt gcgctggcta ttaccgccgg
     8041 tggacctgga gccgtcttct ggatgtgggt tgccgcgttt atcggcatgg cgacctcgtt
     8101 tgccgaatgt tccctggcac aactttataa agaacgtgac gtcaatgggc agtttcgtgg
     8161 cggaccggca tggtatatgg cgcgcgggct ggggatgcgc tggatgggcg ttctgttcgc
     8221 cgtctttttg ctcatcgcct acggcataat tttcagcgga gttcaggcaa acgctgttgc
     8281 ccgagccctg agtttttctt ttgattttcc cccgctggtg acaggcatta ttctcgctgt
     8341 ctttgctctg ctggcaatca ctcgcggtct tcatggcgtc gcccggctca tgcaggggtt
     8401 tgtcccgttg atggcgataa tctgggtact gaccagcctg gtgatttgcg taataaatat
     8461 cgggcaactt ccccacgtca tttggtctat ttttgagagt gcttttggct ggcaggaagc
     8521 ggcaggcggc gcggcgggat ataccttaag ccaggcgatt actaacggtt ttcagcgcag
     8581 catgttttcc aatgaggcgg gaatgggttc gacgccaaac gcggcagcgg cagcggcgtc
     8641 ctggcctccg catccggcag cgcaaggaat tgtccagatg attggcattt ttatcgacac
     8701 cctggtcatc tgtacggcaa gcgccatgct gatattactg gcgggtaacg gcacaaccta
     8761 catgccgctg gaaggtattc agcttatcca gaaggcgatg cgggtgctaa tgggttcctg
     8821 gggtgctgag tttgttaccc tcgtggttat tctgtttgcc ttcagctcca tcgttgccaa
     8881 ctacatttat gccgaaaaca atctcttctt tttacgcctg aacaacccta aagcgatctg
     8941 gtgtttgcgg atctgcacct tcgcaacggt catcggcggc accttgctaa gtcttccgct
     9001 gatgtggcaa ctggcagata tcataatggc ctgcatggct attaccaatt tgaccgccat
     9061 tttactgctc tcgcctgtgg ttcataccat tgccagtgat tatctacgcc agcgtaaact
     9121 cggcgtgcgc ccggtgtttg atccgttgcg ttatccggat atcggtcgcc agctttctcg
     9181 ggacgcgtgg gatgatgttt cgcaggagta atcacaacta tcgatcaact cattctcttt
     9241 ttttgctaaa gtcggcataa atttcctgca aggactggat atgctgattc ttatttcacc
     9301 tgcgaaaacg cttgattacc aaagcccgtt gaccaccacg cgatatacac tgccggagct
     9361 gttagacaat tcccagcagt tgatccatga agcgcggaaa ctgacgcctc cgcagattag
     9421 cacgctgatg cgtatcagcg acaaactggc aggtatcaac gccgctcgct ttcatgactg
     9481 gcagccagat ttcacgccgg agaatgcccg ccaggcgatt ctggcgttta aaggtgatgt
     9541 ctacaccggc ttgcaggccg aaaccttcag cgaagacgat ttcgattttg cccaacagca
     9601 tttgcgaatg ctttccggct tgtatggcgt actgcgcccg ctcgatttaa tgcagcctta
     9661 tcgtctggaa atggggatcc gtcttgagaa tgcccgaggg aaagatctgt atcaattctg
     9721 gggagatatc atcaccaaca agctgaacga ggcgctcgca gcacaaggcg ataatgtggt
     9781 gattaatctg gcgtcagatg agtattttaa atcggtgaag ccgaagaaat tgaatgccga
     9841 gattatcaag ccggtgttcc tcgatgagaa gaacggcaaa tttaagataa tcagcttcta
     9901 cgctaagaaa gcacgcggtc tgatgagtcg tttcattatt gaaaatcggc tgaccaaacc
     9961 agagcaactg actggtttta atagcgaagg ttacttcttt gatgaagctt cctccagcaa
    10021 tggcgaactg gtgtttaaac gctacgagca gcgttaagta tgtagtcaat gccggatgcg
    10081 gcgtgaacgc cttatccggc ctacaaaagc atgcagattc aatatattgc agaattcacg
    10141 taggcctgat aagacgcgcc agcgtcgcat caggcgctga atgccggatt gttacccggc
    10201 atttgtcatt tagcggtgat gtttgcctgg accatgaccg ccgtgatgat catgatgagc
    10261 tttcttatgg tgacgcggcg gtggcggcgg tccgtgtggg tgccagcgat tgcctcgcca
    10321 ttcataatgt tgtttccacc agccgtggtc gcgccagtga ccgccatccc agtaataacc
    10381 gcgattatca cgatcgccta tctgtaattt tactgacggg actaacgtaa tttccgcagc
    10441 ctgcgttgcc atgggagcga ccagaaccag ggaaagtgcg agtacgatag attgcatctt
    10501 tttcacaggt tactcctttt atcattgccc gttatgggtc gatatccgga gattacgcga
    10561 tgagaacggc ggttgttatt ctccgcaatc ctaatctcta agttaccgca tttattgaga
    10621 atttctcctt tttccctgtc gtttctctcc atattttctt cacaaaaaag ccgggctgca
    10681 taaaagcaaa cccggcctga ttgagataat gaatagatgt tactgatgat tcatcatcaa
    10741 tttacgcaac gcagcaaaat cggcgggcag attatgcgaa agcaagggta aatcggcacg
    10801 ttctgccagc tcttttggca gatccaacgt ttcaccgaga atcgcttcca cgctctcttt
    10861 aaatttcgcc ggatgcgcgg tgccgaggaa caagccatat tcgcctggat tcaactggtc
    10921 acgcagcgca cgataagcta cggcagcgtg cggctccgag gtgtagccca gttcttttaa
    10981 ctcacgcatt gtctgttgcg tggtttcatc atccacggct gcataaccca gctctttcag
    11041 ttgccagatt ttgcggcgga acaactcttc cacacgcggc cagttgttcg gctgactcac
    11101 gtccatcgcg ttggataacg tcgcctgagt cgctttgggt gaccactgac cgtcatgcag
    11161 gaaacgtggc acggtatcgt tcacgttggt cgcagcaata aaacgtttca ccggcagacc
    11221 gagtgacttc gccagcagac ccgccgtcaa atcgccgaag tttccgcttg gcaccgagac
    11281 aaccagctgg ttgcgcgcct cctgcggcag ctgcgcaaca gcttcaaagt agtagcaaat
    11341 ctgcgccagc aaacggctga tgttaatcga gttagccgag tttaacccca gcgccacttt
    11401 cagttcttca tcatcaaacg cctgcttcac cagcgcctga caggcatcga aatcgccgtc
    11461 gatggcaaca gtttcgatat tgccgcccaa tgtacagaac agtttttctt gcagtggact
    11521 gattttgcct cgtggataga ggataaccac tttcacattc ggtaaaccgt agaaagcatg
    11581 agccactgcc gctccggtat caccggaggt cgcggtcaga atggtcactg gcttatcgcc
    11641 cgcaatatgg gtcagcattt gtgccataaa gcgaccgccg aaatctttaa atgccagcgt
    11701 tggcccgtgg aacaattcca gacaaccgac atcgctttca acattggcga ccggagccgg
    11761 gaaggcaaac gccgcgcgca cgcgctcttc caggatttcc tgcgggattt catcaccaat
    11821 aaacgccgag aggatcttcg cactgcgggt gacaaaatcc agcttcagca tctcatcaat
    11881 ttcagtcagg ctgaattccg gcaggtcgtg cggaaaaaac agcccctgat ttttgcccaa
    11941 cccctgggtt acggcttgcg caaagctgac ctgctcattg tgatctttca gattgtagag
    12001 tttcatttag ttttccagta ctcgtgcgcc cgccgtatcc agccggcaaa tatgaacaaa
    12061 accttcctga ttttgcaggt agttcttacc caaccagtcg gcaacgcgct gggcggtatc
    12121 cggcttgtca cacagagcaa acaaggtcgg gccggagccg gagataccgc tcgctaccgc
    12181 gccgatttcc gcgaccgcct gccgcgcctg ccggaagcca ggcagtaacc gttcacggta
    12241 gggttctgcg ataacatctt tcatcagctt cgcggcaagc tcaggctgac gggaatagca
    12301 ggcgtgaatg aagcctgcca gatgtcgccc gtgcgcaatg caatcctggc ggcgatactg
    12361 cgccggtaaa atagcccggg cttctgccgt cgagacttta atccccggat acgccagcac
    12421 ccacagccac tcatcaaacc ctggcacttg ctggctgatg atgtcgtttt cttcgatcat
    12481 caactgcata ccaccaagaa aacacggtgc cacgttgtcg taatgaatgc tgccggagat
    12541 acgcccttcc agctcgccca tcagagccag caaacgagtg tcattaagcg gcttgccgca
    12601 gtgttcattc atcgccatca gcgccgcgac caccgaacag gcgctggagc ctaagcccga
    12661 accgatcggc atattctttt ccagagtcat cgccactgga atttgcttgc ccagctcatg
    12721 gcaaaaacgc tcccagcact gataaacgat attttcccgt ggttctgacg gcagcttatc
    12781 ggcaaagcgt ccgaggttgt tgagactgaa tgtctctgcc gcctcaaccg tgactacatc
    12841 tccgagcaat gcaccatcaa cgggtgtcac cgccgccccg agcacatcaa acccgacgct
    12901 catattggca ctggaagccg gggcataaac tttaaccatg tcagactcct aacttccatg
    12961 agagggtacg tagcagatcg gcaaagacac cggcagcggt aacgtcattg cccgcaccat
    13021 atccgcgcag caccaacggc agcggctgat aatagtggct atagaaggcc agggcgtttt
    13081 cgccattttt cactttgaac agcggatcat taccatccac ttcggcaatc ttcacgcggc
    13141 agacgccatc ttcatcaata ttgccaacat agcgcaaaac ttttccttca tcacgggcct
    13201 tcgccacgcg cgcggcaaag agatcgtcga gctgtgacag attcgccata aaagcggcaa
    13261 catcaccctc ggcgttaaac tctgcgggca gcactggttc aatttcaata tccgccaact
    13321 ccagttcacg tcccgtttca cgagcgagaa tcaatagttt acgcgctaca tccataccag
    13381 aaagatcatc tcgcggatcc ggttcggtat aacccatttc ccgcgccagc gtggtcgcct
    13441 cggagaaact catgccttcg tctaacttgc cgaagatata agaaagcgaa cctgaaagaa
    13501 tgccggagaa cttcatcaat tcatcacctg cattgagcag attttgcagg ttctcaataa
    13561 ccggtaatcc agccccaacg ttggtgtcat agaggaattt acgtcgcgat ttttccgccg
    13621 cataacgcaa ctgatggtag taatccatcg acgaggtgtt ggccttttta ttcggcgtga
    13681 caacgtggaa accttcgcgc aggaagtcgg catattgatc cgccactgcc tggctggagg
    13741 tgcagtcaac aatgaccggg ttcagcagat gatattcttt cacgaggcga attaagcgcc
    13801 cgagattaaa cggctctttg gcttgcgcca gttcttcctg ccagttttcc agattaaggc
    13861 catgcacatt ggtgagcaga gccttcgagt tagcaacacc gcagacacgt aagtcgatat
    13921 gtttattctt caaccagatt tgctgacgct tcagttgctc cagcagcgca ccgccaacgc
    13981 caccgacgcc aatcacaaac acttcgataa cctgatcggt attgaacagc atctgatgag
    14041 taacgcgcac gccagtggtc gcatcatcgt tatttaccac gacagagatt gagcgttcag
    14101 aagatccctg agcaatggcg acaatgttga tattggcgcg ggccagcgcg gcaaagaatt
    14161 tcgccgagat cccacgcaag gtgcgcatac catcacctac caccgagata atggccagcc
    14221 gttccgtcac cgccagcggc tccagtaagc cttctttcag ttccaggtag aactcttcct
    14281 gcattgcccg ttcagctcgc acacagtcgc tttgcggaac gcagaaactg atactgtatt
    14341 cggaagatga ttgcgtaatc agcaccacgg aaatacgggc gcgtgacatt gcagcaaaga
    14401 cgcgcgccgc catgccgacc attcctttca tccccgggcc ggaaacgctg aacattgcca
    14461 tgttattcag attggaaatg cccttgaccg gtaattcgtc ttcatcacgg ctggcaccaa
    14521 tgagcgtacc tggagcttga ggatttccgg tatttttaat caggcacggg atctggaact
    14581 gggcgatggg ggtaatggtg cgggggtgaa gaactttagc gccgaagtag gaaagctcca
    14641 tcgcttcctg gtaggacatc gatttcaaca acctcgcatc gggcacctga cgcgggtcgc
    14701 aggtatagac cccgtcaacg tccgtccaaa tctcgcaaca atcggcgcgt aaacaggcag
    14761 ccagcaccgc cgcggagtag tcggaaccgt tgcgtccaag taccaccagt tcgccttttt
    14821 cattaccggc ggtgaaacct gccatcagca ccatgtgatc agccggaata cgacttgccg
    14881 caatacggcg ggtggactca gcaatatcga cggtagattc gaggtaatgc cccactgcca
    14941 gtagtttttc gaccggatcg ataacggtaa cgttgtgacc acgcgcttct aacacgccgg
    15001 ccataatggc gatcgacatt ttctcgccac ggcaaatcag cgcagcgtta atgctatccg
    15061 ggcactgccc taacaaacta atgccatgca ggacatgttt tatttgggca aattcctggt
    15121 cgacgaaagt tttcaattgc gccagcggga atcccggctg ggcggcggcg agtcccgtca
    15181 gaagttcggc aaaaatacgt tcggcatcgc tgatattggg taaagcatcc tggccgctaa
    15241 tggttttttc aatcatcgcc accaggtggt tggttatttt ggcgggggca gagaggacgg
    15301 tggccacctg cccctgcctg gcattgcttt ccagaatatc ggcaacccgc agaaaacgtt
    15361 ctgcatttgc cactgatgta ccgccgaact tcaacactcg catggttgtt acctcgttac
    15421 ctttggtcga aaaaaaagcc cgcactgtca ggtgcgggct tttttctgtg tttcctgtac
    15481 gcgtcagccc gcaccgttac ctgtggtaat ggtgatggtg gtggtaatgg tggtgctaat
    15541 gcgtttcatg gatgttgtgt actctgtaat ttttatctgt ctgtgcgcta tgcctatatt
    15601 ggttaaagta tttagtgacc taagtcaata aaattttaat ttactcacgg caggtaacca
    15661 gttcagaagc tgctgtcaga gactcttttt ttaatccaca cagagacata ttgcccgttg
    15721 cagtcagaat gaaaagctga aaaatactta ctaaggcgtt ttttatttgg tgatattttt
    15781 ttcaatatca tgcagcaaac ggtgcaacat tgccgtgtct cgttgctcta aaagccccag
    15841 gcgttgttgt aaccagtcga ccagttttat gtcatctgcc actgccagag tcgtcagcaa
    15901 tgccatggct cgttcgcgta aagcttgcag ttgatgttgg tctgccgttg tgtcactttt
    15961 cgtcggttgt tgtattaatg ttgctaattg atagcaatag accatcaccg cctgccccag
    16021 attgagcgaa ggataatccg ccaccatcgg cacaccagta agaacgtcag ccaacgctaa
    16081 ctcttcgtta gtcagcccgg aatcttcacg accaaacacc agcgcggcat ggctcatcca
    16141 tgaagatttt tcctctaaca gcggcaccag ttctactggc gttgcgtagt aatgatattt
    16201 cgcccgactg cgcgcagtgg tggcgacagt gaaatcgaca tcgtgtaacg attcaaccag
    16261 tgtcgggaaa actttaatat tatcaataat atcaccagat ccatgtgcga cccagcgagt
    16321 ggctggctcc agatatgcct gactatcgac aatccgcagt tcgctaaacc ccatcgtttt
    16381 cattgcccgc gccgctgccc caatattttc tgctctggcg ggtgcgacca gaataatcgt
    16441 tatacgcata ttgccactct tcttgaccaa atgaccgcga acagggtgat cactgtcaac
    16501 ttattacgcg gtgcgaattt acaaaccctt aacgtaagtc gcagaaaaag ccctttactt
    16561 agcttaaaaa aggctaaact atttcctgac tgtactaacg gttgaattgt taaaaaatgc
    16621 tacatatcct tctgtttact taggataatt ttataaaaaa taaatctcga caattggatt
    16681 caccacgttt attagttgta tgatgcaact agttggatta ttaaaataat gtgacgaaag
    16741 ctagcattta gatacgatga tttcatcaaa ctgttaacgt gctacaattg aacttgatat
    16801 atgtcaacga agcgtagttt tattgggtgt ccggcccctc ttagcctgtt atgttgctgt
    16861 taaaatggtt aggatgacag ccgtttttga cactgtcggg tcctgaggga aagtacccac
    16921 gaccaagcta atgatgttgt tgacgttgat ggaaagtgca tcaagaacgc aattacgtac
    16981 tttagtcatg ttacgccgat catgttaatt tgcagcatgc atcaggcagg tcagggactt
    17041 ttgtacttcc tgtttcgatt tagttggcaa tttaggtagc aaacatgcag accccgcaca
    17101 ttcttatcgt tgaagacgag ttggtaacac gcaacacgtt gaaaagtatt ttcgaagcgg
    17161 aaggctatga tgttttcgaa gcgacagatg gcgcggaaat gcatcagatc ctctctgaat
    17221 atgacatcaa cctggtgatc atggatatca atctgccggg taagaacggt cttctgttag
    17281 cgcgtgaact gcgcgagcag gcgaatgttg cgttgatgtt cctgactggc cgtgacaacg
    17341 aagtcgataa aattctcggc ctcgaaatcg gtgcagatga ctacatcacc aaaccgttca
    17401 acccgcgtga actgacgatt cgtgcacgca acctgctgtc ccgtaccatg aatctgggta
    17461 ctgtcagcga agaacgtcgt agcgttgaaa gctacaagtt caatggttgg gaactggaca
    17521 tcaacagccg ttcattgatc ggccctgatg gcgagcagta caagctgccg cgcagcgagt
    17581 tccgcgccat gcttcacttc tgtgaaaacc caggcaaaat tcagtcccgt gctgaactgc
    17641 tgaagaaaat gaccggccgt gagctgaaac cgcacgaccg tactgtagac gtgacgatcc
    17701 gccgtattcg taaacatttc gaatctacgc cggatacgcc ggaaatcatc gccaccattc
    17761 acggtgaagg ttatcgcttc tgcggtgatc tggaagatta atcggcttta ccaccgtcaa
    17821 aaaaaacggc gctttttagt gccgttttta tttttcaacc ttatttccag atacgtaatt
    17881 catcgtccgt tgtaacgtct ttactggctt tcattttcgg cagtgaaaac gcataccagt
    17941 cgatattacg ggtcacaaac atcatgccgg ccagcgccac caccagcaca ctggttccca
    18001 acaacagcgc gctatcggca gagttgagca gtccccacat cacaccatcc agcaacaaca
    18061 gcgcgagggt aaacaacatg ctgttgcgcc agcctttcaa taccgcctgc aaataaatac
    18121 cgttcattaa cgccccaatc agactggcga ttatccatgc cacggtaaaa ccggtatgtt
    18181 cagaaagcgc cagcaagagc aaataaaaca tcaccaatga aagccccacc agcaaatatt
    18241 gcattgggtg taaacgttgc gcggtgagcg tttcaaaaac aaagaacgcc ataaaagtca
    18301 gcgcaatcag cagaatggca tacttagtcg cccggtcagt taattggtat tgatcggcgg
    18361 gtgtcgtcac tgcgacgcta aacgcgggga agttttccca gccggtatca ttgcctgaag
    18421 caaaacgctc accgagatta ttagcaaacc agctgctttg ccattgcgcc tgaaaacctg
    18481 actcactaac ttcccgtttg gctggtagaa aatcacctaa aaaactggga tgcggccagt
    18541 tgctggttaa ggtcatttcg ctattacgcc cggtaggcac cacagaaaga tcgccggtac
    18601 cacttaaatt caatgccata ttcagcttca ggttctgctt gcgccagtcc ccttcgggta
    18661 aagggatatg cacgccctgc ccgccttgct ctaacccggt gccgggttca atggtcagcg
    18721 ccgttccgtt aacttcaggc gctttcacca caccaatacc acgcgcatcc ccgacgctaa
    18781 tcacaataaa tggcttgcca aaggtgatat ttggcgcgtc gagttcgcta agacgcgaaa
    18841 catcgaaatc ggcttttagc gttaaatcac tgtgccagac ctgaccggta taaatcccta
    18901 tcttgcgttc ttccacgttc tgattgccat caaccatcaa tgactcaggt aaccaaaaat
    18961 ggataaaact tcgtttccgc tccacggttt tatcctcttc ctgcaccgta taaagctcgg
    19021 tcacaggaat agcgataagc ggcccaacga gtttttgcgg cccgctggta ctttggcgaa
    19081 tcgcatcttc cacatcgcta cggtaatcgg cacgttcgac aatcacctgc cgaatcagca
    19141 ttatcggaat caacaacagc aatactgcac caaacagggt ggtcattttc cagaacaggg
    19201 gggatttcaa catagtcttc tcctttgcaa tggcagcagg atacgaaggc tatgtgggaa
    19261 gaatttgaag ctatgtgaag tgacggtgaa gtcgaagcga ggccagcacg ccgccttcct
    19321 gcacgttgcg cagcgtgact tcgccgttaa acaaacgggc gacctcactg acgaacgcca
    19381 accccagacc gctgcttttt tgcccatttg cacgcggcaa agagtaaaag cgttcaaaaa
    19441 tacgtgacag cgcgtagtaa ggaataccac taccggtatc cagcacctta agcgtgacgt
    19501 gttcatgatc cacttcggcg cttagcgtga tgcaaccgct ctcgggagta aaatcgatgg
    19561 cgttatccag taaattcccc agcgcctgct ccagtaacgc cggttcagca gcaacgttaa
    19621 cctcagtagg cataacatgc aaagtgatgt ttttttctgc caactgcacg gtgcgcgctt
    19681 cgctgacgcg gcgaaataat gccgccacat caacagcagt cagaacgact tcctgacgat
    19741 tctccagtct tgcctggcgt agtaacgttt ccaccagcgc ctgcattcgc gcattttgcg
    19801 tcagaatgtt gtcagtaaaa cgagctacca cttccggcgg cggaccttcg cgtaaaattt
    19861 ccgccgcgcc acgaatcgcc gccagtgggc tttttagctc atgggttaac gcataaacat
    19921 actgctcaat atagtttttc ccttccagct tcacgcgcat actttccagc gcctgcgcaa
    19981 gcttacgcaa ctcgctactg ccgagttcag gaagaggaac gggcttattg tcagtgacgg
    20041 aatcggcata gcgagtgagc cgggcaatgg agcggttgat ccaccaaacc atgcctgcgc
    20101 caatcaccag tgcaatcccc agcaaaatgg cgctggccca taaaattcgc cgctcgctgc
    20161 gcttaatgac cggagccatc gccgcgttcg gtttgcctac gctcaaaacg ccaataagcc
    20221 gcgagccgtc cataatcggc gcggcaacat acatcaccga actttcggga tcggcaggat
    20281 tttgcaacgt gctgcgcgca ccatactgac cacgcaacgt tagccagacg tcattccagc
    20341 gcgaataatc ctgcccaacg gctttatttg ccgaatcgaa caatactttg ccctgcgcat
    20401 cggtcatata gacatgatat tcattgcgca ctttgttaat gccaccgata ttggcgcgaa
    20461 acgggcgatg ttgtagctga ttaaaggcct gcgccagttg cccatgcgtt gggtccccag
    20521 agagcaaatc gggacgcgcc agctccgcca gcaacgttgc ggtgtcgatc agcgtcccct
    20581 ccgttgctct tcgcacgccc ggtttaactt ctttgacaaa aatagccagt acgaaccagg
    20641 ccgccaccgc caccagtaaa aaatagccca gcaacaaccg catgccgata cgcattacag
    20701 gcccctcagg ctatatccca tgccgcgatg agtattaatc ggtgaaagat cggggttgat
    20761 ggcgcgcagc ttggcacgca acgttttaat gtgggtatcg acggtgcgat cgtaggtgtc
    20821 ctgcgcatct tcccatacgc tatccattag ttgctggcgg gaccagacgc ggcctggcga
    20881 cttgagtaac gtcttcagca ataaaaactc ataccgcgtc agcgttaatg gcgtgtcaaa
    20941 ccagctgatc tgcgccgcgg gttcattcaa ttcaaaatga ccaatacgga tgacgggaga
    21001 cggcgacgag aacttcttca cccgacgcag taaggtgcgc accctggcgc acacttcgcg
    21061 gggtgaaaac ggtttagcca catagtcgtc agcgccaatt tccagcccaa gcaggcgatc
    21121 gacctcttca cttcgggccg tcaggaacag tacaggtaac gccggatgga gcgccagtaa
    21181 ctggcggcac aattcaaagc cgctgatatc aggcaaacca acatcgagaa tcatgacgtc
    21241 gggaaccagc tggcgagctt tatccagcac cggcaagcct cgctcaaaga cctcgacggc
    21301 aaaaccttcc tgctgcaaca tgtagaccag cgtgtcggct atcccttgct catcttccac
    21361 taaccagacc gtttcccgtt gcataaatcg cctctgttat tgccgccacg gcatgacagg
    21421 aaccgcgcta atcgcgtttt tcggcgaacc ttctacaact ttgtcggagt aagccagata
    21481 agcgagcgcg ttgcgtttgg cgtcataaaa gcgcacgacc tgtaacgact taaagaccag
    21541 ggacgtgcgt tttttgaata ctacctcgcc ctgagctttg ccgtttttaa tacgatccga
    21601 cagttcaatc ggcccgactt gctgacaaga aatagccgca tcggaggtat cttccgccag
    21661 acccaatccc cctttaatac cacctgtttt cgcccggctc acataacagg tgacattttt
    21721 cacatcggga tcgtcaaagg cttccacaac aattttgtga tccgggccga tcattttaaa
    21781 tacggtgtcg accgaaccaa tctcttctgc atgagccaat ggccccagca ttatcaggct
    21841 taaagacagg atcaaatgct tgtatttcat attgttacca tttttatcta ttacacgagc
    21901 aattagttcg tcacggtaaa taacatattg attaagaaca aaatctcaat acttttattt
    21961 ccgtcaagac ctaaaacata ctctactaag aaaaaaacac tgaatgctaa aacagcaaaa
    22021 aatgctatta tccaattacc tgatgtcagg tgctcgttgt tgaaaggatg aggatatttt
    22081 atggatcagg ccggcattat tcgcgacctt ttaatctggc tggaaggtca tctggatcag
    22141 cccctgtcgc tcgacaatgt agcggcgaaa gcaggttatt ccaagtggca cttacagaga
    22201 atgtttaaag atgtcactgg ccatgctatt ggcgcgtata ttcgtgctcg tcgtttgtcg
    22261 aaatcggcgg tcgcactacg cctgactgcg cgtccgattc tggacatcgc actgcaatac
    22321 cgcttcgact ctcaacagac atttacccgc gcattcaaga agcagtttgc ccagactcct
    22381 gcactttacc gccgttctcc tgaatggagc gcctttggta ttcgcccgcc gctgcgtctg
    22441 ggtgaattca ctatgccaga gcataaattt gtcaccctgg aagatacgcc gctgattggt
    22501 gttacccaga gctactcctg ttcgctggag caaatctctg atttccgcca tgaaatgcgt
    22561 tatcagttct ggcacgattt tctcggcaac gcgccgacca ttccgccagt gctctacggc
    22621 ctgaatgaaa cgcgtccgag tcaggataaa gacgacgaac aagaggtatt ctataccacc
    22681 gcgttagccc aggatcaggc agatggctat gtactgacgg ggcatccggt gatgctgcag
    22741 ggcggcgaat atgtgatgtt tacctatgaa ggtctgggaa ccggcgtgca ggagtttatc
    22801 ctgacggtat acggaacgtg catgccaatg ctcaacctga cgcgccgtaa aggtcaggat
    22861 attgagcgat actacccggc agaagacgcc aaagcgggag atcgcccaat taatctgcgc
    22921 tgtgaactgc tgattccgat ccgccgttaa cgctgcagct catctaatgc aggggcgtcc
    22981 agatgcgaga tgtcccctgc ggtttcaacg acccagccag acgccagcca caggctttcc
    23041 tgataatcca cgcgcgaaat agaacagtta cgcagacgta agcggcgctc tgcccatgct
    23101 ggtaatccga gaatcgtact caccaggcat cccagtgcaa taccatgact taccagcagc
    23161 ggtcggcttc cctgcggtaa gtcccggcaa gattccagtg ccgcattgat gcgatcgctg
    23221 agctcctgca ttgactcgcc ttcaggaata cgcccgtcaa cggtgccatt gaccagctgc
    23281 cgacgccagt tctcttcttc ttcggtcaga gaatcgatat gtcttttttc cagcacaccc
    23341 atgtttaatt cacgcaggcg agaatcaaag atgatgtcac agccgcaggc ctgggcgatg
    23401 atttccgccg tacgccgggt gcgtcctaaa tcgctactga tgatatgcgt aatgccaagc
    23461 tctttggcac gcgtcgccac ctgcatcgcc tgttgctcac ctttggcggt cagcgggctg
    23521 tcagactggc cctgaatacg tcgctcggcg ttccactgcg tttcaccgtg gcggactagg
    23581 tatacctgta acatgctttt tttccgtata ctgctgtaac gttaattttc tgagtaatgc
    23641 tgattatgca ccaagttgtc tgtgcgacca ccaatcccgc taaaattcag gccattctgc
    23701 aggcatttca cgagatcttc ggcgaaggat cctgccatat tgcatccgtt gccgtcgaga
    23761 gcggtgtacc ggaacagccc tttggcagtg aggaaacgcg cgctggcgca cgaaatcggg
    23821 tagcaaatgc ccgccgttta cttccagagg ctgatttttg ggtggcgatt gaagctggca
    23881 ttgatggcga cagcactttc agttgggtgg tgattgaaaa cgccagccag cgcggcgagg
    23941 cgcgttcggc tactttgccg ttgcctgcgg tgattctgga aaaagtgcgt gaaggtgaag
    24001 cgcttggtcc cgtgatgtcg cgttatactg gtattgatga gattggtcgt aaggaagggg
    24061 cgatcggcgt gtttaccgcc gggaaactca ctcgcgccag cgtctatcac caggcggtga
    24121 tcctggcgct gagtccgttt cataatgccg tgtattaagc gcctgatgcg acgctgccgc
    24181 gtcttatcat gcctaccaaa catattgaaa ttacgggtat ttgtaggacg gataaggcgt
    24241 tcacgccgca tccggcattc ggtgcacgat gcctgatgcg ccacgtctta tcaggcctac
    24301 aaaatcaatc gcttttcagc aacacctctt ccagccactg gcgcagctcg acgggcgcgg
    24361 ctttcaggct gttagatcca cgcgtaatcg tcgcgatgcc tgcgccgagt tcatttttta
    24421 actcacgctg gctcatttcg ccgcgcaaca gctcttcgac aatacgcacg cgagtcccca
    24481 acgcttcgcg ctcatctggc gtcagcatca ggtttaacaa cggtaaatgg agatcgtttt
    24541 ggtaggcatt cttaagcagg tcgacaaaac gtaaccactc ctggtgacgc tgttctgcca
    24601 tcgctgctga atagggtgat tgttgggcca taatatgtcg ccattgttag cgggggaagc
    24661 aaaatgcctc ccccggttgt actcgctaaa gagtacgata gcatatcata aacgtgtgga
    24721 tcagtaacga cgtccccatt ccgtggcgct catcaacgtc ggtttatccc ccatgaaata
    24781 gcggtagtaa gcgtcataag ccagcacgtt cttcacataa ccgcgcgtct cggagaatgg
    24841 aatactctcg acaaatgcca ctgcgtcgat acgcccggcg ctgttgccaa gccaggttcg
    24901 cacccgccct ggtccggcgt tataagctgc tgaggagaaa atacgattat tgccaaattg
    24961 ctgataaaca tattgcaggt aactggtgcc aatgttgata ttcgtttccg gatccagcaa
    25021 ttgcccaggg ctgctataac caggaataga gaacatcttc accgtatggg tcgccgtgcc
    25081 cggcataatc tgcatcaggc cgctggcccc taccggtgat ttcactttcg gattccaggc
    25141 gctctcctga cgggcaatcg ccatcgcata gctttgcggg atctccttcc cactggtgta
    25201 acgtttgaaa agatcgttgt aagccagcgg gaatcgctct tccagatgat cccaaagctt
    25261 cccggcgatc gttgcctgaa cgctaagatc ccaccattgg ttgttgaaag cataccgcgc
    25321 cagttgagct tgctctgttt ttgacttgct cttcaccaga ttggcccact cgctacgcgc
    25381 ggtattatcg agattccagt acatcaactc gcgcacgcgc gccatctccg gcccctcagt
    25441 cagggtgctg tcaacgttct gcggcgcttt atcaatcttc agctcatact cttcgccgat
    25501 gcgttgcgct gcaaccatcg ggtagaaacc acgctgttgc atgagttgat gcaaaatctc
    25561 ttttgcttca gcttcacgtc cgcgttccag caataaatcc gcttgccagt aacgccattc
    25621 atctttctct ttcgcctcca tcggcaaacg cgccagccag gtattcaggc cgcgacgatc
    25681 gccagtgcca agcgccattc gcacacggcg ttcaataagc gaagtagatt gcgagcgcat
    25741 aatggcgtca tcgcgccatt tcgcctgctc gtcggtgaca tcgttgccca tcaaacgcca
    25801 ggcgacgata tcgcgcagct cctgaatctg atcttcatta agctgctgcg cctgggcaag
    25861 cgatgggatc atcagccgtg cattttcagc atcctgccgc gccacactgg caaacgccac
    25921 cgccgccatt tgacgggtaa aatcggtcgc tccggttgta cgcgcgaagg tcagtaccgt
    25981 attagggttg ttcgccagtg aaatgattgc cgaggcgata gtctggtaat cggcaggcat
    26041 ctgccctgcc agcactgtta ccaggccagt gttacccgct ttcatcgcca gacggatacg
    26101 ctctaaatac gccagcggat cttgtttacc tgacgcacgc cagacgctaa ataacttgtc
    26161 acaggcgtta ggctggctct tgccggttag ccacagctct ttcgcccctt gccaggcttc
    26221 ttcactctgc ccggtgttcc atttcgcata gtagtaatta cattgcgctt cggtagttcc
    26281 gggcttttcc gggctaaagg ctaacaagcc acgccagtct tcacgccgcg ccagttcatt
    26341 gacgaaacga gattgcagcg tgcgagcggg aggaagcgtg gggttagcgc gaacaaagtt
    26401 agtgaccgtc accgccggtt gattcatcag gtcatcggtg atctggcggt attccaggta
    26461 gggataaagc ggataatcct tcagtccagg catcatttgt tcgaccacat ccatttgtcg
    26521 attatcccag gcctgcttga tttgcgcgta acgactacgc tgctcatcca gtgagtcggc
    26581 tcgcgccacg ctgctgaccg tcagcagaca gacaccggca gccaacagcc gccaggtaac
    26641 ttgtttggct ttttccacaa gcgcatcctc taagtgtaaa tacatcaatg cagcgtcatg
    26701 ccgcgtaata gatttatgct aaccagtcat tgccgtttac gccacgttac ggacactttt
    26761 ttacttttac tgcgaaggtg atcgaccaac tgggattagg cggggaaaaa ggctacactt
    26821 cgcctttgaa aactcatcat cacgataaac atagaggcga agtccaacgt ggctcaattc
    26881 gtttatacca tgcatcgtgt cggcaaagtt gttccgccga aacgtcatat tttgaaaaac
    26941 atctctctga gtttcttccc tggggcaaaa attggtgtcc tgggtctgaa cggcgcgggt
    27001 aagtccaccc tgttgcgcat tatggcgggc attgataaag acatcgaagg tgaagcgcgt
    27061 ccgcagccag acatcaagat tggttatctg ccgcaggaac cgcagctgaa cccggaacac
    27121 accgtgcgtg agtccattga agaagcggtt tcagaagtgg ttaacgccct gaaacgcctg
    27181 gatgaagtgt atgcgctgta cgccgatccg gatgccgatt ttgacaagct ggccgctgaa
    27241 caaggccgtc tggaagagat cattcaggct cacgacggtc ataatctgaa tgtacagctg
    27301 gagcgtgcgg cggatgcgct acgtctgccg gactgggacg cgaaaatcgc taacctctcc
    27361 ggtggtgagc gtcgtcgcgt ggcgttgtgc cgcctgctgc tggaaaaacc agacatgctg
    27421 ctgctcgacg aaccgaccaa ccacctggat gccgaatccg tggcctggct ggaacgcttc
    27481 ctgcacgact tcgaaggcac cgttgtggcg attacccacg accgttactt cctcgataac
    27541 gttgcaggct ggatcctcga acttgaccgc ggtgaaggta ttccgtggga aggtaactac
    27601 tcctcctggc tggagcagaa agatcagcgc ctggcgcagg aagcttcaca agaagcggcg
    27661 cgtcgtaagt cgattgagaa agagctggaa tgggtacgtc aaggtactaa aggccgtcag
    27721 tcgaaaggta aagcacgtct ggcgcgcttt gaagaactga acagcaccga atatcagaaa
    27781 cgtaacgaaa ccaacgaact gtttattcca cctggaccgc gtctgggcga taaagtgctg
    27841 gaagtcagca acctgcgtaa atcctatggc gatcgtctgc tgattgatga cctgagcttc
    27901 tcgatcccga aaggagcgat cgtcgggatc atcggtccga acggcgcggg taaatcgacc
    27961 ctgttccgta tgatctctgg tcaggaacag ccggacagcg gcaccatcac tttgggtgaa
    28021 acggtgaaac tggcgtcggt tgatcagttc cgtgactcaa tggataacag caaaaccgtt
    28081 tgggaagaag tttccggcgg gctggatatc atgaagatcg gcaacaccga gatgccaagc
    28141 cgcgcctacg ttggccgctt taactttaaa ggggttgatc agggtaaacg cgttggtgaa
    28201 ctctccggtg gtgagcgcgg tcgtctgcat ctggcgaagc tgctgcaggt tggcggcaac
    28261 atgctgctgc tcgacgaacc aactaacgac ctggatatcg aaaccctgcg cgcactggaa
    28321 aacgccctgc tggagttccc tggctgcgcg atggttatct cgcacgaccg ttggttcctc
    28381 gaccgtattg ccacgcacat cctggactac caggatgaag gtaaagttga gttcttcgaa
    28441 ggtaacttta ctgagtacga agagtacaaa aaacgcacgc tgggcgcaga cgcgctggag
    28501 ccgaagcgta tcaagtacaa gcgtattgcg aagtaatgcg taaaatgccg gatgcggcgc
    28561 gaacgcctta tccggcctac aaaaccagca atttcaatac gttgtaaaac tgtaggcctg
    28621 ataagacgcg gtaagcgtca catcaggcat tgagcaccga atgccggatg cggcgtgaac
    28681 accttttccg gtctacaaca gccttagcat cctcaatttc gtggcgggtc acccgtgcag
    28741 gttttttcaa accttgttta atctcgacag cctcttccag agaggttttc aactcatcaa
    28801 aaaaactcat tttatacctc gcctttgagc agtttcatca cggttatctc tgctccccca
    28861 tcatctcccg caccagctcc acgcagcgca ggaaacggct gtcgtaatct tcctcttcaa
    28921 cccgcacaaa ttcgatattg ttctcttcga gcatctccac cagcaagttc tggaactctt
    28981 tgcgatccac cgaactgccg aggctgcgta aaccatccgc cacccacggc gtgttgttct
    29041 ccagcaggat caccagatca aaacggtatt catcaatcag cgcctgcacg aacggatgct
    29101 cgcgtccctc gtactttttg cagaacgcct gagtggtgac aaaatcggta tcgataaatg
    29161 ccactttatt ggcatatttc accgcaaaat caatgtattg agcgtggccc agcgcgattt
    29221 tatcgtagtc ggaatactgc aatgcgatct catcaccgcc gaggtgtgaa aagacataat
    29281 cgcgaccata ttcccacgca ctggtggtat tgaagatatt ggcaagtttg tttaccaggg
    29341 tggatttacc gctcgactcg ccgccaagga tcgccacggt acgcacaaag aacggcttca
    29401 cttccgtagg aatatattcc cagtagcgga acgggttttc gcggatctgt gcaccgctga
    29461 tactcataaa ggtacgtttc ggatcgacca gcaccgtttc gatccccaga tgttccatat
    29521 actgtggcgc atcggcttct tccgaggtgt agatcaggtc cggctgaatc cccttttcag
    29581 ccataaactt tttgatgccg ttgctccaca catcccagcc gtgcggatac ggctccatgc
    29641 cctcttcgtt gaaagcatga atgcgaatat ttttctgata tttaaaagtt tgcaataacc
    29701 agcgcagacg atccggtacg gtcggctgct gcgacatggc actgtcttcg aacaacgcgc
    29761 ggtcacgggt atcgtcaaaa cccataatga tatgcagctc gtcaacctgg ctacaggcgc
    29821 gctggataag gtagatatgt ccggtatgca gtgggtagaa cttaccgaat accacaccga
    29881 tcgttttctt ctgccgggga aactcaagcc ccaaaaaacg gtgcaacgcc tccagctttt
    29941 gcgcgctggg gcttttgatt ttggcattca gtaactggct taaataccct ttggtcatac
    30001 cgctggcatc agctacctgc tgtagcgtgc agccctgttg cttgatggca gttttcaggt
    30061 aatcaaatga cgacatatct ccctccgtat ttctcattat aagtcgtcga acacgctaag
    30121 cgcgtcggag agttttttaa cgccaaaaat ctgcatccct tccggcgctt ttttcggtac
    30181 gttagccgcc ggaacaatcg cccggcgaaa accgtgtttc gccgcttcag agattcgttc
    30241 ctgaccgctg ggcaccgggc ggatctcccc tgccagcccg acttcaccaa acaccaccag
    30301 atcctgcggc agcggtctgt cgcgcaggct ggaaaccatc gccagcagta acgctaagtc
    30361 ggcactggtt tcggttacct tcacgccgcc gaccacgttc acaaacacat cctgatcggc
    30421 catttgcaga ccaccgtgac ggtgcaagac agccagcagg attgccagac ggttttgttc
    30481 cagccccact gccacgcggc gcggattcgc catcatcgag tgatcgacca gcgcctgaat
    30541 ctccaccagc aacggacgcg ttccttccca caccaccatc acggagctgc cggaggtcac
    30601 ttcatctccg cgacttaaga aaattgccga agggttgctg acttcacgca gcccctgttc
    30661 ggtcatcgcg aagacgccca gctcattcac cgcgccgaag cggtttttat ggctgcgcaa
    30721 ggtgcgaaaa cgggagtcgg catcgccatc cagcagcacc gaacagtcga tacagtgttc
    30781 cagcacttta gggccagcca gcgaaccatc tttggtaacg tgaccgacca tgacaatcgc
    30841 cacgccgcgc gttttggcga aacgtgtcaa ataagccgcc gtttcacgta cctgcgccac
    30901 gctgcctggc gatgattgta catccgccat atgcatcacc tggatcgagt caatcaccat
    30961 cagcttcggt tgctcttctt cggcaatcag gcagatctgt tcgatgctgg tttccgacaa
    31021 catattgaga ttgtcagtcg gcagtccaag gcgatgagcg cgcattgcca cctgttgcag
    31081 cgactcttcg ccggtgacat acagcgtttt catctgctgg gccagtttgc acagcgtttg
    31141 caatagcaat gtggatttcc cggcacctgg gttaccgcca atcagaatgg cgcttcccgg
    31201 caccaccccg ccgcctaata cgcggtcgaa ctctttaaat ccggttgaaa aacgcggcag
    31261 ctcttcaagg ctgatatcgg agagtttctg gacttttgcc accccggcgc taccggcata
    31321 gccgctgaga cgctcgttac gcgccaccgt tggcgacgca gcaagacgca cctcggtgat
    31381 ggtgttccag gcatgacagg cactgcactg cccctgccag cgcggataat cggccccgca
    31441 ttcattacaa acaaaggcgc gtttgggagc ttttgccacg gtgtacctca ttaatgctgt
    31501 gccgcccgca ggatgacggg cgggcaatta cttctgattc aggctgccag agaggatgca
    31561 gaataccccc atcaggtcag cgtgacggat ggtgacttcc gtcttttcat tcacttttgg
    31621 cttggcatgg taggcaatcc ccagccctgc cgctttgatc atcggcaggt cattggctcc
    31681 atcgccaatc gccacggtct gcgccagcgg gatttcatac tcctgcgcga ggcgagtcag
    31741 agttttcgct ttgtactgcg cgtctacgat gtcgccgatc acattgccgg taaatttacc
    31801 gtccatgatc tccagttcat tggctaccac ggctgtcagg cgcagcttgt cgcgcaggta
    31861 ttcagcaaag aaagtaaagc cgccggaggc aatcgccact ttccagccca gcgtttccag
    31921 cttgagcacc agttgcgtta agcctggcat cagcggcaga ttttcacgca cctgttgcag
    31981 aatattggcg tcagcgcctt tcagcgtcgc cacacggctg cgcaggctgg cggtaaaatc
    32041 gagttcgccg cgcatcgccc gttcggttac ttccgccacc atctcgcccg ttccggccag
    32101 tttggcaatt tcatcaatac attcaatctg gatggcggtg gaatccatgt ccatcaccag
    32161 caaacccggc gtgcgcaggt gcgggatttt ccccagcggg gcgacatcca gttgcgcttc
    32221 atgggccagg cgcgtagccc gtgcggtgag tgaacctgcc agacgaatca cctgataatc
    32281 ttccacgcac caggcggcaa caatcaccat cgccgcaccc agtttgctct ggtattgggt
    32341 cagacgctgt ttatccagcc cacgaccata cagcagccag ccgctacgac ctgcgtggta
    32401 atccagtggc atcacttcat caccacttaa tgaaagaggc agacccggcc ataaagagac
    32461 atcttcaggc aggtcgcacc aggtaatgtt aggcattaag gctcctgtaa aatcgttcga
    32521 agcagggaaa ataacgcatg aggctacctt gtatccattg cttctggcaa cattaagtct
    32581 caaattttca aagggtggaa gatggctcgc acaaaactga aattccggct gcatcgggca
    32641 gtgattgtcc tgttctgcct tgccttgtta gtggcactga tgcagggggc gtcatggttt
    32701 agtcaaaacc accagcgaca gcgtaatcca cagctggaag aactggcccg caccctggcg
    32761 cgtcaggtga cgctgaacgt tgcaccgctg atgcgtaccg actcaccgga tgaaaaacgc
    32821 attcaggcga tcctcgatca gttaacggat gaaagccgta tcctcgacgc gggtgtgtat
    32881 gacgaacaag gcgatcttat cgcccgttct ggcgaaagcg tcgacgtgcg tgacaggctg
    32941 gcgctcgacg gtaaaaaagc aggcggctat tttaaccagc agattgtcga gccaattgcg
    33001 ggtaaaaacg gaccgctcgg ctatctgcgc ctgacactcg acacccatac gctcgccacc
    33061 gaagcccaac aggtggataa caccactaac attttacgcc tgatgttgct gctctcactg
    33121 gcaatcggcg tagtgctgac ccgcacgctg ctacagggta aacgcacccg ctggcagcaa
    33181 tcgcccttcc tgttaaccgc cagcaaaccg gtgccggaag aggaagaaag cgagaaaaaa
    33241 gagtgaccca ttactacaag aaaggaaatc gttatgtcca cattacgcct gctcatctct
    33301 gactcttacg acccgtggtt taacctggcg gtggaagagt gtatttttcg ccaaatgccc
    33361 gccacgcagc gcgttctgtt tctctggcgc aatgccgaca cggtagtaat tggtcgcgcg
    33421 cagaacccgt ggaaagagtg taatacccgg cggatggaag aagataacgt ccgcctggcg
    33481 cggcgcagta gcggtggcgg cgcggtgttc cacgatctcg gcaatacctg ctttaccttt
    33541 atggctggca agccggagta cgataaaacc atctccacgt cgattgtgct caatgcgctg
    33601 aacgcgcttg gcgtcagcgc cgaagcgtcc ggacgtaacg atctggtggt gaaaaccgcc
    33661 gaaggcgacc gcaaagtctc aggatcggcc tatcgcgaaa ccaaagatcg tggcttccac
    33721 cacggcacct tgctgctcaa tgccgacctt agccgcctgg caaactatct caatccggat
    33781 aaaaagaaac tggcggcgaa aggcattacc tcagtgcgtt cccgcgtgac caacctcacc
    33841 gagctgctgc cggggatcac ccatgagcag gtttgcgagg ccataaccga ggcctttttc
    33901 gcccattatg gcgagcgcgt ggaagcggaa atcatctccc cggacaaaac gccagacttg
    33961 ccaaacttcg ccgaaacctt tgcccgtcag agtagctggg agtggaactt cggtcaggct
    34021 ccggcattct cgcatctgct ggatgaacgc tttagctggg gcggcgtgga actgcatttc
    34081 gacgttgaaa aaggccatat cacccgcgcc caggtgttta ccgacagcct caaccccgcg
    34141 ccgctggaag ccctcgccgg gcgactgcaa ggctgcctgt accgcgcgga tatgctgcaa
    34201 caagagtgcg aagcgctgtt ggttgacttc ccggaccagg aaaaagagct acgggagttg
    34261 tcgacgtgga tagcgggggc ggtaaggtaa ctcgctgatt aaaataccaa aggaagggaa
    34321 tatgatcgat ctggaaaacc aggagcggga gattatcaat ttaatgttaa gccagcgtat
    34381 ttcctggcta gctgccgtgc gtatcaggca taaattatcg cttgctgaag taagtaaaat
    34441 gttaggtatc tcgattaatt ctctaaaata gatcgaaaag acggaacggt ttagctcaaa
    34501 catcaaaagc aaaatggcag aaatttatgg ttgtccacca gagttgctga tatgcccttc
    34561 c
//
LOCUS       DAPGOF010000034        34286 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.034, whole genome shotgun sequence.
ACCESSION   DAPGOF010000034 DAPGOF010000000
VERSION     DAPGOF010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.034"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(113..1054)
                     /gene="fabY"
                     /locus_tag="RHV13_001419"
                     /gene_synonym="yiiD"
     CDS             complement(113..1054)
                     /gene="fabY"
                     /locus_tag="RHV13_001419"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HDV1240972.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1099..1536)
                     /gene="dtd"
                     /locus_tag="RHV13_001420"
     CDS             complement(1099..1536)
                     /gene="dtd"
                     /locus_tag="RHV13_001420"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDV1240973.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(1533..2405)
                     /locus_tag="RHV13_001421"
     CDS             complement(1533..2405)
                     /locus_tag="RHV13_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HDV1240974.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2399..2998)
                     /gene="yihX"
                     /locus_tag="RHV13_001422"
     CDS             complement(2399..2998)
                     /gene="yihX"
                     /locus_tag="RHV13_001422"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HDV1240975.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3097..3882)
                     /locus_tag="RHV13_001423"
     CDS             complement(3097..3882)
                     /locus_tag="RHV13_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDV1240976.1"
                     /translation="MSLTELTGNPRHDQLLTLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(3916..4812)
                     /gene="yihV"
                     /locus_tag="RHV13_001424"
     CDS             complement(3916..4812)
                     /gene="yihV"
                     /locus_tag="RHV13_001424"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HDV1240977.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDAK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQVGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATGGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTQSFLSLFV"
     gene            4980..5876
                     /gene="yihU"
                     /locus_tag="RHV13_001425"
     CDS             4980..5876
                     /gene="yihU"
                     /locus_tag="RHV13_001425"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HDV1240978.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRSVLFGENGVCESLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            5900..6778
                     /gene="yihT"
                     /locus_tag="RHV13_001426"
     CDS             5900..6778
                     /gene="yihT"
                     /locus_tag="RHV13_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HDV1240979.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLSASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            6795..8036
                     /gene="yihS"
                     /locus_tag="RHV13_001427"
     CDS             6795..8036
                     /gene="yihS"
                     /locus_tag="RHV13_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HDV1240980.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWDYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            8174..9076
                     /locus_tag="RHV13_001428"
     CDS             8174..9076
                     /locus_tag="RHV13_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HDV1240981.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHANPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            9275..11311
                     /gene="yihQ"
                     /locus_tag="RHV13_001429"
     CDS             9275..11311
                     /gene="yihQ"
                     /locus_tag="RHV13_001429"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HDV1240982.1"
                     /translation="MDTPRPQLPDFEFHQNNDSFTLHFQQRLILTHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNKEGVQFLAYI
                     NPYVASDKDLCEEAAKRGYLAKDVAGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGISAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAQTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGETFHGGEITVEAPIGKPPVFYRADSEWAALFASLKNI"
     gene            11357..12742
                     /locus_tag="RHV13_001430"
     CDS             11357..12742
                     /locus_tag="RHV13_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1240983.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            12782..14185
                     /locus_tag="RHV13_001431"
     CDS             12782..14185
                     /locus_tag="RHV13_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1240984.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDIAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEEKTASVTL"
     gene            14252..14944
                     /gene="ompL"
                     /locus_tag="RHV13_001432"
     CDS             14252..14944
                     /gene="ompL"
                     /locus_tag="RHV13_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HDV1240985.1"
                     /translation="MKNINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSAGS
                     GGAVYLDVNYKFTPWFNLTVRNRFNHNNYSSTDLNGDLDNNDSYEIGNYWNFIITDKF
                     SYTFEPHYFYNINDFNSGNGTKHHWEITNTFRYRINEHWLPYIELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(15035..16300)
                     /locus_tag="RHV13_001433"
     CDS             complement(15035..16300)
                     /locus_tag="RHV13_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1240986.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(16402..17382)
                     /locus_tag="RHV13_001434"
     CDS             complement(16402..17382)
                     /locus_tag="RHV13_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HDV1240987.1"
                     /translation="MMTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(17390..18100)
                     /locus_tag="RHV13_001435"
     CDS             complement(17390..18100)
                     /locus_tag="RHV13_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HDV1240988.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KSRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(18318..20141)
                     /gene="typA"
                     /locus_tag="RHV13_001436"
     CDS             complement(18318..20141)
                     /gene="typA"
                     /locus_tag="RHV13_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HDV1240989.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            20514..21923
                     /gene="glnA"
                     /locus_tag="RHV13_001437"
     CDS             20514..21923
                     /gene="glnA"
                     /locus_tag="RHV13_001437"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HDV1240990.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            22097..23146
                     /gene="glnL"
                     /locus_tag="RHV13_001438"
     CDS             22097..23146
                     /gene="glnL"
                     /locus_tag="RHV13_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HDV1240991.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            23158..24567
                     /gene="glnG"
                     /locus_tag="RHV13_001439"
     CDS             23158..24567
                     /gene="glnG"
                     /locus_tag="RHV13_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HDV1240992.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            24679..24789
                     /locus_tag="RHV13_001440"
     CDS             24679..24789
                     /locus_tag="RHV13_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HDV1240993.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(25018..26391)
                     /gene="hemN"
                     /locus_tag="RHV13_001441"
     CDS             complement(25018..26391)
                     /gene="hemN"
                     /locus_tag="RHV13_001441"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDV1240994.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(26580..27086)
                     /gene="yihI"
                     /locus_tag="RHV13_001442"
     CDS             complement(26580..27086)
                     /gene="yihI"
                     /locus_tag="RHV13_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HDV1240995.1"
                     /translation="MKPSSPSRSKGHAKARRKTREELNQEARDRKRQKKRRGHAPGSR
                     AAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELLE
                     TDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQED
                     MMRLLRGN"
     gene            27668..28300
                     /locus_tag="RHV13_001443"
     CDS             27668..28300
                     /locus_tag="RHV13_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDV1240996.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(28682..31468)
                     /gene="polA"
                     /locus_tag="RHV13_001444"
     CDS             complement(28682..31468)
                     /gene="polA"
                     /locus_tag="RHV13_001444"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDV1240997.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            31832..32764
                     /locus_tag="RHV13_001445"
     CDS             31832..32764
                     /locus_tag="RHV13_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HDV1240998.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            complement(32805..34235)
                     /locus_tag="RHV13_001446"
     CDS             complement(32805..34235)
                     /locus_tag="RHV13_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HDV1240999.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSHFQMQISFDNGAPDLNIKPGQKVAFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT         8346 a         8771 c         8407 g         8762 t
ORIGIN      
        1 gaagccaacg aagaataaaa atttatagag ttcttattca atataaccga aatagccaag
       61 gactaatata aatgacctga atgagagagg cagcaccgcc cctctccatc agctactctt
      121 cttcgttccc gccctcttca tacgggccaa atggcttcgc gggcagaacg atatacgtgc
      181 cttcaaacac tgcacccggc gtctcgtcgc caaagatttc gacctgcatc tgcacccgtg
      241 cttttcgtcc gcgcgccaga cggtcgagat cgccgcttaa agcaccaagg tcggctaccg
      301 catggggttt accgctaatc ggtttgctgt agcggatatg cgcatccgcc agaataatcg
      361 ttccgcccag atggcgttca cgcagcatca gccagataag cccccaaccg gtgagcgtcg
      421 ccagcgagaa taaactcccg gcaaacagcg tatggtgcgg attctgatta ccggtttctg
      481 gcatggtggt gataaatttt tgcccggtat attgctgaat gcgcacgccc attttttcac
      541 taagcgggat atgttcgtac cacgcctgtt gcagctgcgc gcaccagtcg ccgcgatgca
      601 gaatgtcatc cagagtggcg acgggcttaa tcatcaaaaa atggcgaatc ggcgtggtgg
      661 ttggcgtggt gatttctccc tgattaacaa accccagctt ggcgaaaaac tccaccgcgt
      721 cttcacgggc gctacaggtc acgcgcttaa cgccttcctg acgcgccacc gactccaggg
      781 tcatcgccat cagcgtgcct aaccctttgt cctgcacgtc gggatgaacg gccataaagc
      841 gaatggacgc ttcattgtcg gcattaatat acagtcggcc taccgccacc agattaccct
      901 gctcgtcgac gaccatctgg tgatgcgcca tcgcatccca cgcgtcgcgt tccgaacctt
      961 ttggttgatg caggggctta cgcaacattt cccagcgaaa ctgatagtaa cgctctaatt
     1021 cttcttctgt ttgtggaacc cgaaggtgat acatagcgat actctctctt gtttcccgtg
     1081 acaaccctgg aagctggctc atacctgcaa ccagaatgtc acggggccat cattgaccag
     1141 cgatacctgc atatccgcag cgaagcgtcc tgtttgcgtg ttcatctctt gctgacggca
     1201 gcgttcgacg aaatagtcat ataacgcctc tgcgcgatcc ggtgatgcac ctttggagaa
     1261 acttggacgc atcccccgtt cggtatctgc ggcgagggta aactgggaaa ccaccagcac
     1321 actgccgccc gcctgttgca cgttgaggtt catcttgcct tcggcatcgc taaagatgcg
     1381 atagccgagc acacgctcgc acagacggtt tgctttctgt tcgtcgtcat ccttttcgac
     1441 acccaataac accaaaagtc ccgcgccaat ttcgcccgtc acttctccct ccacggtgac
     1501 gctggcacgg gttacgcgtt gaattaatgc aatcatggtt cgtcgtcttc ttcttgttca
     1561 gctgcttgtt ttagtttgcg gtattccccg agagtgacag taatttccgc gccaagcaag
     1621 acgatacacc atgtccagta gacccaaaca aagagaatgg ggatcaccgc cagcacaccg
     1681 taaatgagct gatatgacgg gaacatggtg atataaagcg cgaaaccctt ctttcctgct
     1741 tcgaacagga gcgcggcgac aaacgcgccg acaatcgcgt cgcggttagg tacgcggatg
     1801 gtaggaacaa tgctgtacag caaccagaag gagatccacg acaacagcag cggaaaaata
     1861 cgcagcacat tgtcgatgac agtattgaga tcgctcgccc agcgcaggga gagcaaatag
     1921 gaactgatcg ccagactggc ccctgccagc agcggcccca gcgttaaaat catccagtac
     1981 acggcgaacg agtaaatttt tggtcgcgct cgtttactgc gccagatggt attcaacgcg
     2041 ctatcgatgg agtacatcag caataacgcg gtgacgatca gcccgcacgc cccaacggcg
     2101 gtcatcttgt tggaattggc aacaaattgt tcgatatacc gctgaataac atcgccagta
     2161 gcaggcagaa agttggcaaa aataaagtga cgtaactgaa tgctgacgtc ggaaaacatg
     2221 ggaaaagcgg cgaaaagcgc aaaaacaacg gcaaccagcg gcactaatga gagcaacgac
     2281 acataggcaa ggttacctgc cagggttgtc atgttgtcct catcaatgcg ttgccagagt
     2341 agttttagcc aggcccatag tggacgggta cggtgcctgg ctttgtcctg aatggttttt
     2401 agcataacac cttcgcgaaa tagtccggga tggtggtttt atctttcacc agaatactgg
     2461 taatgcctag ctgattagct ccttctatat tatcggcgtt atcgtcgaaa aagaccgtat
     2521 cgctgggtga aaaaccttcc gcctgcaaaa catgctggta aattcgagct tcaggtttgc
     2581 gcatccccag atcttgcgac agatagatat ggtcagcagc atcacgaatt tccgggtatt
     2641 cttccggcca gaaggtggta tgcaggcggt tggtattgga aagcaccacc acgcgatgtc
     2701 cctgctcacg cagtttatgc atgatggcga tcacttccgg gcgcagcgcg acaaacaccg
     2761 cctgccagcc gtgagagaac tgctcgtagc ttagcggcag ggccatctca tgacacagcg
     2821 cctctgcgaa cgcttcgtcg ctaatttccc cacgctcatg ctgatgaaac gcctccccca
     2881 tgtggaaact cttcttaagc gtagccagtg gaacacgcgt taaatcgctc caggctccca
     2941 gcacacggtt aaagtcgata tcgacaatca cattacctaa atcaaagata tagagcattt
     3001 ttgcctcctt tcgcgccaca agaaattaac tgtagcggga aaggatgggt ttggctatgt
     3061 ggcaaggtat gaagccaggt caacggtcac aaaaatctac gcgtcttctt ggggaagaat
     3121 gatttcaatt tggctgtctt gtaagcgtga ttttagcgca gcgggcggca gctcgtcggt
     3181 aaagagcgcg gtgacctgtg ccacgttacc aatttcaacc gccgccgaag catgatactt
     3241 agtgtgatcg gcgaccagca ggatatttct cgcgtgcgcc atcatcgttt tcaccacgtt
     3301 agcttcgttt acatcaaact ccatcaacgc gccatcgctc tcgattgcgc ctacgcttgt
     3361 taccagataa tcagcacgaa aatcggccac aaaggacgcc gctgaagggc caatgatccc
     3421 gctattatga gagcgcaacg taccgccggg taccatcact tcaaagcgcg ggttgtggta
     3481 aagaatatgc gccacacgca ggctgttggt gattatccgc aaatgattat ggttaagtaa
     3541 cgcccgggca acatgctcaa cggtcgtacc aatggtgata aatattgttg aaccatcagg
     3601 aatatagtct gccaccgctt cggcaatcgc ttttttttcc tcggtttgcg aaacctcacg
     3661 ctgctcgaac gccgtattaa cgacgctgga agcccgaccc gcgccaccgt gatggcgcgt
     3721 aatcaggcct tgctcgctta atttacgaat atcccggcgc accgtctgcg tggagacatc
     3781 cagcagattt gccagctcat caatattcat atacccacgc tcggcgatca gcgtgaggag
     3841 ttggtcgtgc cgcgggttac cggttagttc ggtaaggctc atgaaaattc ctcgaaaaac
     3901 catcaccctg gcattttata caaaaagtga caaaaaagat tgggtttgat cacagtcagg
     3961 gatcccggct cgtccaccgg gacgcgtgca tttcaacgcc gctacaccgc tggcgaagcg
     4021 gactgactcc gctaaatccc cacctgttgc cagcgccacc gccaatgcgc cgtgaaaaac
     4081 atcacccgca cctgtggtat ctaccacatc aactttgaag gccggttgat gttgacgccc
     4141 gccattttcc agccagtcac agccagcgct gccctgggtc acatagacat gaccatttgt
     4201 gagcgtttgt gcctgtttta gcgcactggc catctctttc acgcccgtta agcgcgccag
     4261 acccggttct gaaaaggccg catgatcgct taatgccacc agctcactga tatcctgcgg
     4321 cgtaatgtcc ccgtccagaa cggtcatcac gcccacctgc cgggccagag tgaaggcttt
     4381 tttagcgccg tcgtgccagc gtacatctgc cagcacaaca tcccactgag agaaatcaat
     4441 ttcctccaac cactctgcgt caggcagcag gtccgggctg gggtagttaa tgattatccg
     4501 ctcgcctttg gcatccacca tgatggcgga ttgcgatgat ttcgcctggt tataccgttt
     4561 ggtgtaacgg gtgttaacgc cccaggattc cagctctgcc agcaggctgt tgcccgtgtc
     4621 gtcatcacct acgcgaccaa taaaatcaac ctgcgccccc agccttgccg ccgcaaccgc
     4681 tgccgtcgca gctggcccgc caccaacttc cgtataattt ctcgccacgt atttaccgct
     4741 ctccgtcggt aacccttcca cgtaatagat gcgatccatc acggttatac ctacacaagc
     4801 aacacgaatc atggtcattc cttaaacatt ttgggatgcg ttcattttaa tttcaccaat
     4861 tgtttaaaaa gtgacggctg tcaatttttt gactataaat tacaaatacg atcaaaaaca
     4921 gacaatcaaa gtgtgtcata aatgacaaaa agtgacatca ctgtattcag gagaaggtta
     4981 tggcagcaat cgcgtttatc ggtttagggc aaatgggttc gccaatggcg agcaatttat
     5041 tgcagcaagg gcaccaactt cgcgtctttg atgtgaatgc cgaggctgtg cggcatctgg
     5101 tagacaaagg cgcgactccc gccgccaatc cggcgcaggc cgctaaagat gccgaattta
     5161 tcattaccat gttgccgaat ggcgatctgg tgcgcagcgt gttgttcggt gaaaacggcg
     5221 tttgcgaaag cttatctacc gatgcgctgg tcattgatat gtcgaccatc catccgctgc
     5281 aaaccgataa attgattgcc gatatgcaag ccaaaggctt cagcatgatg gatgttccgg
     5341 taggccgtac ttctgcaaat gccattaccg gtactctgtt actgctggct ggcggcaccg
     5401 ctgaacaagt tgaacgtgcc acgccgatcc tgatggcgat gggcagtgag ttgatcaacg
     5461 ctggcggtcc gggcatgggg atccgcgtta agctcatcaa caactatatg agcatcgcgc
     5521 tcaatgcgct ttcggcagaa gccgccgttt tgtgcgaagc cctgaatctt cccttcgatg
     5581 ttgccgtcaa agtgatgagc ggtaccgccg ccggtaaagg ccacttcacc acttcctggc
     5641 caaacaaagt cctcagcgga gatctttctc ccgccttcat gatcgatctt gcccataagg
     5701 atcttggcat cgcccttgat gtcgccaacc agctgcatgt gccaatgccg ctgggggccg
     5761 cctcacggga ggtttatagc caggcgcgcg cagcgggtcg cggtcgccag gactggtccg
     5821 ccattctgga acaggtccgt gtcagtgccg ggatgactgc caaagtaaaa atgtaacgac
     5881 tggataaagg aataaatgaa tgaataagta caccatcaac gacattacgc gcgcctcggg
     5941 cggttttgcc atgctggcgg tcgatcagcg cgaagccatg cggatgatgt ttgccgcggc
     6001 tggggcaccc gctccggtag ccgatagcgt tttaactgat ttcaaagtta acgctgcaaa
     6061 ggccctctcg ccttatgcct cggcgattct ggtagatcaa caattctgct accgccaggt
     6121 ggttgagcaa aacgcgattg ccaaaagttg cgccatgatt gtcgccgccg atgagttcat
     6181 tcctggcaac ggtattccgg tagatagcgt ggtcattgac cgcaaaatca atccgctaca
     6241 gatcaaacaa gacggtggca aagccttaaa actgctggtg ctgtggcgta gtgatgaaga
     6301 tgcgcagcaa cgcctggata tggtgaaaga gtttaacgaa ctgtgccact cacacggcct
     6361 ggtaagcatt attgagccag tcgtccgccc accacgtcgt ggcgataaat tcgatcgcga
     6421 acaagcgatc atcgatgccg ccaaagagct gggcgacagt ggcgctgacc tctacaaagt
     6481 tgaaatgccg ctttacggta aaggtccgca acaagagctt ctcagtgctt cacaacgtct
     6541 gaatgaccat atcaatatgc cgtgggtgat cctctcttcc ggtgtcgacg aaaaactgtt
     6601 cccgcgtgcc gtacgcgtgg caatgacagc gggtgcatcg ggcttcctgg ctggtcgtgc
     6661 agtctgggca tcggtcgtcg gtttaccaga taacgagctg atgctgcgtg acgtttgcgc
     6721 accgaaatta caacaacttg gcgatatcgt cgacgaaatg atggctaaac gccgctaaga
     6781 tgaggataaa aggaatgaaa tggtttaaca ccctaagcca caaccgttgg ctggaacagg
     6841 aaaccgaccg catctttgat tttggtaaaa attccgtagt gccgactggt tttggctggt
     6901 taggcaataa agggcaaatc aaagaagaga tgggcaccca tctgtggatc acagctcgta
     6961 tgttgcacgt ttattccgtt gctgcggcga tgggtcgacc tggcgcttac gcgttggttg
     7021 atcacggtat taaagccatg aacggcgcac tgcgtgataa aaaatatggc ggctggtatg
     7081 cctgtgtgaa tgacgagggc gtggtggatg cctccaaaca gggctatcaa cacttctttg
     7141 ctctgctggg tgccgccagc gccgtcacaa cgggtcaccc ggaagcgcgc aagctgctcg
     7201 attacaccat tgaaattatc gagaaatact tctggagcga agaagagcag atgtgcctgg
     7261 agtcctggga cgaagccttc agcaaaactg aagagtaccg cggcggcaat gccaatatgc
     7321 acgcagtgga agctttctta attgtttatg acgtaacgca tgacaaaaaa tggctggatc
     7381 gcgcgattcg cgtggcttcc gtgattatcc acgacgtcgc aagaaataat cattatcgcg
     7441 ttaacgaaca cttcgatacc cagtggaatc cgctgccgga ttacaacaaa gataacccgg
     7501 cgcaccgctt ccgcgcgttc ggaggtacac caggccactg gatcgaatgg ggccgtttaa
     7561 tgctgcacat ccacgcggcc ctggaagccc gttgcgaaca accgccagca tggctgctag
     7621 aagatgccaa aggtctgttt aacgccaccg tgcgcgatgc ctgggcaccc gatggtgcgg
     7681 acgggattgt ttataccgtt gactgggaag gaaaaccggt ggtccgcgaa cgtgtgcgtt
     7741 ggcctatcgt cgaagcgatg ggtacggcct acgcgctcta caccgtcacc ggcgatcgcc
     7801 agtatgaaac ctggtatcag acatggtggg actactgcat caaatacctg atggactatg
     7861 aaaatggttc ctggtggcag gagctggatg cggacaataa ggtcaccacc aaagtctggg
     7921 acggcaaaca ggatatttat cacctgctgc attgcctggt gatcccgcgt atcccgttag
     7981 cgcccggcat ggctccagcg gttgcagcgg gtctgctgga tattaatgcg aaataactga
     8041 tctgaaaata gggtgcgggt tttcccctca ccctaaccct ctccccaaag gggcgagggg
     8101 accgtccggt gcagtttttg ggtttgacat cagcctcacg ggaggctgta tgtcgttaat
     8161 taaggtgcct tgtatgcaaa tcaccaatat gcattgcagt ggacagaccg tttcacttgc
     8221 cgcgggggat tatcacgcga ccattgtgac cgtcggtgct ggtctggctg aattaacctt
     8281 tcagggttgt catttagtca tcccgcacaa acccgaagag atgccgctgg cgcatctggg
     8341 taaagtgctt attccgtggc cgaaccggat cgccaacggc tgttaccgct atcaggggca
     8401 ggaatatcag ttacccatta atgaacatag ttcaaaagcc gctattcacg ggctgcttgc
     8461 ctggcgagac tggcaaatta gtgaattaac cgccaccagc gttaccctga cagcttttct
     8521 gccgcccagt tacggctatc cgtttatgct ggcttcacaa gttgtttatt cgctcaacgc
     8581 acacacgggt ctttccgttg agatcgcaag ccagaatatc ggcactgtcg cagcgcctta
     8641 tggcgtgggt attcatcctt atttaacctg caacttaaca tctgttgatg aatatctgtt
     8701 ccagcttccg gcaaaccagg tttatgccgt cgatgagcac gcgaatccga caacgcttca
     8761 ccacgttgat gagctggatt taaatttcac gcaggcgaaa aaaatagccg cgacaaaaat
     8821 cgaccacacc tttaaaaccg ctaatgattt gtgggaaatg accattaccc atccacagca
     8881 ggcgctttcg gtaagccttt gttccgatca accctgggtg caggtttata gcggtgaaaa
     8941 attacagcgt cagggactgg cagttgaacc tatgagttgc ccgcctaacg cctttaattc
     9001 aggtatcgac ttattattac tggaaccagg aaaaccgcac cgcctgtttt ttaatattta
     9061 cggacagaga aaataacttg tattgattag cagtcaaaat cattgaagaa catttcactc
     9121 gctaaaggca acattatgta tcttcaattt attaagttaa aaacagctca gcttctgtaa
     9181 agtttttata tatcagtttt atgagaatca ttttacataa gcctgtaata acaggaaggc
     9241 aacaatctcg ttctcaactc tatggagatt atttatggat acgccacgcc cacaattacc
     9301 agattttgaa tttcatcaga ataacgacag ttttacccta cattttcaac aacgtcttat
     9361 tttaacccat agcaaagata atccttgttt atggattggc tcaggtatag cggacatcga
     9421 tatgttccgc ggcaatttca gcattaaaga taaactacag gagaaaattg cgcttaccga
     9481 cgccatcgtc agccagtcac cggatggttg gttaatccat ttcagtcgtg gttctgacat
     9541 tagcgccacg ctgaatatct ctgccgacga tcagggccgt ttattgctgg aactacaaaa
     9601 cgacaacctt aaccacaacc gtatctggct gcgccttgcc gctcaaccag aggaccatat
     9661 ctacggctgc ggcgaacagt tttcctactt cgatctgcgc ggcaaaccgt tcccgctgtg
     9721 gaccagtgaa caaggcgttg gtcgcaacaa acaaacctat gtcacctggc aggccgactg
     9781 caaagaaaat gcgggcggcg actattactg gactttcttc ccacagccta cgtttgtcag
     9841 cacgcagaag tattactgcc atgttgataa cagttgctat atgaacttcg actttagtgc
     9901 cccggaatac catgaactgg cgctgtggga agacaaagca acgctgcgtt ttgaatgtgc
     9961 tgacacatac atttccctgc tggaaaaatt aaccgccctg ctgggacgcc agccagaact
    10021 gcccgactgg atttatgacg gagtaacgct cggcattcag ggcgggacgg aagtgtgcca
    10081 gaagaaactg gacaccatgc gtaacgcggg cgtgaaggtc aacggcatct gggcgcagga
    10141 ctggtccggt attcgtatga cctcttttgg caaacgcgtg atgtggaact ggaagtggaa
    10201 cagcgaaaac tacccgcaac tggattcgcg tatcaagcag tggaacaaag agggcgtgca
    10261 gttccttgcc tatatcaacc cgtatgttgc cagtgataaa gatctctgcg aagaggcggc
    10321 aaagcgcggt tatctggcaa aagatgtcgc cggtggcgac tatctggtgg agtttggcga
    10381 gttctacggc ggcgttgtcg atctcaccaa tccggaagcc tacgcctggt tcaaggaagt
    10441 gatcaaaaag aacatgattg aactcggctg cggcggctgg atggctgact tcggcgagta
    10501 tctgcccacc gacacgtacc tacataacgg catcagcgcg gaaattatgc ataacgcctg
    10561 gcccgcgctg tgggcgaaat gtaactacga agcccttgaa gaaacgggca agctcggcga
    10621 gatcctcttc tttatgcgcg ccggttctac cggtagccag aaatactcca ccatgatgtg
    10681 ggcgggtgac cagaacgtgg actggagtct ggacgatggc ctggcgtcgg ttgtcccggc
    10741 ggcgctatct ctggcaatga ccggacatgg cctgcatcac agcgacattg gcggttatac
    10801 caccctgttt gagatgaagc gcagcaaaga gctgctgctg cgctggtgcg atttcagcgc
    10861 cttcacgccg atgatgcgca cccacgaagg taaccgtcct ggcgacaact ggcagtttga
    10921 cggcgacgcg gaaaccatcg cccatttcgc ccgtatgacc accgtgttca ccaccctgaa
    10981 accttacctg aaagaggccg tcgcgctgaa tgcgaagtcc ggcctgccgg ttatgcgccc
    11041 gctgttcctg cattacgaag acgacgcgca gacctattcc ctgaaatatc agtacctgtt
    11101 aggtcgcgac attctggttg ctccggtaca cgaagaaggt cgcagcgact ggacgctcta
    11161 tctgccagag gataactggg tccacgcctg gaccggagaa accttccacg gtggagaaat
    11221 aactgttgaa gcgcccatcg gcaagccgcc ggtcttttat cgcgccgaca gcgaatgggc
    11281 tgcactgttc gcctcgttaa aaaacatcta atcatgcccg cccgtgggga aactcacggg
    11341 caaatggaga ataacgatga gtcacatcac aacggaagat ccagcaactt tacgcctgcc
    11401 ctttaaagag aaactctctt acggtattgg cgacctggcc tctaacatcc tgctggatat
    11461 cggtacgctt tatcttttga agttttatac cgacgttctg gggctgccag gcacctatgg
    11521 cggcattatc tttttgattt caaaattctt tactgcgttt accgatatgg gtaccggcat
    11581 tatgttggat tcccgacgca agatcggtcc aaaaggtaag ttccgtcctt ttattctgta
    11641 tgcgtcattc ccggtcacct tactggcgat cgctaacttt gtcggcacac cgtttgatgt
    11701 caccggtaaa acggtgatgg ccactattct gtttatgctt tacggactgt ttttcagcat
    11761 gatgaactgc tcctacggcg caatggttcc cgccattacc aaaaacccca acgaacgcgc
    11821 ctcgctggca gcatggcgtc agggcggcgc tacgctgggc ctgctgctgt gcacggtggg
    11881 attcgtgccg gttatgaatc ttatcgaggg taatcagcaa cttggctata tcttcgccgc
    11941 cacgctgttt tcactgtttg gtctgctgtt tatgtggatc tgctattcgg gcgtgaaaga
    12001 gcgttatgtc gaaacccaac ctaccaatcc ggcgcaaaag ccaggcctgt tgcaatcttt
    12061 ccgcgcaatt gccggtaacc gcccactgtt cattttgtgc attgccaacc tctgtacctt
    12121 aggggcgttt aacgtcaagc tcgccattca ggtctattac acccagtacg tacttaacga
    12181 tcccatcctg ttgtcatata tgggattttt cagcatgggc tgtattttca tcggcgtgtt
    12241 cctgatgcct ggcgcagtca ggcgttttgg taagaagaag gtctatatcg gcggcctgct
    12301 gatttgggtg ctgggcgatc tgctcaacta tttcttcggc ggcggttcgg tcagcttcgt
    12361 ggcgttctcc tgcctggcat tcttcggctc agcgtttgtt aacagcctga actgggcgct
    12421 ggtttccgac accgtcgagt acggcgagtg gcgtaccggc gttcgttcgg aaggcacggt
    12481 ctacaccggc ttcaccttct ttcgcaaagt gtctcaggcg ctggctggtt tcttccccgg
    12541 ctggatgctg acgcaaatcg gttatgtgcc gaacgtcgca caggctgacc acaccattga
    12601 agggttgcgc cagctgatct tcatctaccc aagcgcactg gcggtagtca ccattgtggc
    12661 gatgggctgc ttctacagcc tgaacgagaa gatgtatgtc cgcattgttg aagaaataga
    12721 agcccgtaaa cgcacggcgt aataataaac aacgccctgc ggggcgttat aaggagtgat
    12781 tatgtctgac cataatccac tgacattaaa actgaatctg cgggaaaaaa tcgcctatgg
    12841 tatgggcgac gtcggttcga atttaatgct ctgcatcggt actctgtatc tcctcaaatt
    12901 ttataccgat gaattaggga tgcctgctta ctatggcggg attatctttc tggtggcgaa
    12961 gttttttacc gcgtttaccg atatgctcac cggattttta ctcgactcgc ggaaaaatat
    13021 tgggcctaaa ggcaaatttc gtccctttat tttatatgca gcagttccgg cggcgttaat
    13081 tgcaacgctc cagtttatcg ccaccacctt ttgtttgccg gttaaaacga cgattgccac
    13141 cgcgctgttt atgatgtttg gactttcata cagtctgatg aactgctcgt atggtgcgat
    13201 gatcccggca attaccaaaa acccgaatga gcgcgcgcag ctcgcggctt accgccaggg
    13261 tggtgcaacc atagggctat tgatttgtac cgtggcgttt attccgttgc agtcgctttt
    13321 ttctgactca accgtcggtt atgcctgtgc ggcacttatg ttctccattg gcggctttat
    13381 ttttatgatg ctgtgctaca gaggcgtcaa agagcattat gtggacatag cgccaaccgg
    13441 acataaagcc agtattctca aatctttttg cgcgatattt cggaatccgc cattgctggt
    13501 tttatgcatt gctaacctgt gtactctggc ggcatttaat atcaaactgg cgattcaggt
    13561 ctattacacc cagtatgtgc tgaatgatat taatttattg tcgtggatgg gattcttcag
    13621 tatgggatgc atcctcgtcg gcgtattact ggtgccagta actgtaaaat gttttggtaa
    13681 aaaacaggtt tatttagctg gcatggtgct atgggcggtg ggtgatatac tgaattattt
    13741 ctggggaagt aactctttca ctttcgtcat gttctcttgt gtcgcctttt ttggcacggc
    13801 ctttgtaaac agcctgaact gggcactggt accagatacc gtagattacg gtgaatggaa
    13861 aaccggtatt cgtgccgaag gttctgttta taccggttat accttctttc gtaaaatttc
    13921 tgccgcactt gctggcttct tgccaggcat tatgctgacg caaattggtt atgttcccaa
    13981 catcgcgcaa agcgatgcga cattacaggg tttgcgtcag ctcattttta tctggccttg
    14041 tgcactggca attattgctg cattgacgat gggattcttt tacacactca atgaaaaacg
    14101 gtttgcgttg attattgagg aaatcaacca acgcaaaaat aaagagatgg cgacagaaga
    14161 aaaaacggct tccgtaacgt tataaatgtc aatagccgct atttccatcc tggtgggtgg
    14221 cggcctccct acgtttaaaa atggacttat tatgaaaaat attaatgcaa taattttatt
    14281 atcttctctg acctctgcct cagtattcgc tggcgcatac gtcgaaaaca gggaggcata
    14341 taatctcgcc tccgatcagg gcgaggttat gctacgtgtg ggttataact tcgatatggg
    14401 cgcgggtatt atgttaacca atacctacac ctttcagcga gaagatgaac ttaaacatgg
    14461 atataacgaa attgaaggct ggtatccgtt atttaaacca accgataaat taaccatcca
    14521 gcccggtggc ttaattaatg ataagagcgc cggttccggt ggtgctgttt atctggatgt
    14581 taactacaaa tttactccgt ggtttaacct gacagtgcgc aaccgtttta accataataa
    14641 ctacagctca accgatttaa acggtgatct ggataataat gacagctacg agattggcaa
    14701 ctactggaac tttattatta ccgataagtt ctcatatacc tttgagccgc attattttta
    14761 taacattaat gatttcaata gcggcaatgg cacgaagcat cactgggaaa tcactaacac
    14821 cttccgttac cgcattaacg agcactggct gccctatatt gaattgcgct ggttagatcg
    14881 caacgtggaa ccgtaccacc gcgagcaaaa ccagattcgt atcgggacga agtatttctt
    14941 ctgatgtgaa actgccggat gcggcgtaaa cgccttatcc ggcctacggt tgatacggtt
    15001 ttcgttggcc ggattaaaca catccggcca ttaattaagc ttcagccgcc agcatctctt
    15061 tcttacgttg gctaatacgc ttaaccagca ttacggaaac caccgcacca cagaatgcga
    15121 agcaggccat aatgccaaac acgattttgt agccaataat ccccggattt aaatccagaa
    15181 tgtagccata cagactgaag cagaacatcg ccggggcgta accaataaag ctacccaacg
    15241 ccatcgccgc gcctgtttta ttttcagcaa tttttgcttc gccgataggt gcaaaaaata
    15301 cggctcgctg tgtaaagact atcgcgccaa agcccagcgt acatgccatc cctaaataga
    15361 ccggcatact ttcgtgcggc agcataatca acagtacgag cgcagcggta ctgatgataa
    15421 aggtgtagca tagatattta ctcggcgatt tcaggatctt atctgaaatc atgccaccaa
    15481 tcggtccacc aatcattttc agacagtatt ggttaatgat gccgtaagcc cccaccagcg
    15541 caacgggcaa tagatagatg tttttcagga atggaatgaa gaatgtcagg ccacagtaaa
    15601 ccgcgtaaac gaagaagacg ttgaaagcga taagccagat ggttttgtct ctcagcaccg
    15661 aggtcatact ggtgttttgc gatgctccgt cttctttttt cacctcaacg gacggtgcct
    15721 cttctttgtc attcaggaca aagaaaataa taatgcctac ggcaatcaca ataagggaat
    15781 agaaccagat gcctgcttta aaacctaata agccactgcc aaaccaggta aatactgcca
    15841 acgcagaaaa tgccaccacg gtatcgacaa tgccacgccc tgtttcgaag aagccaaaca
    15901 accgaccttg ttgttcgcta ttgcccaatc gacttaccga cttgagcaag accggccagt
    15961 tcatcatgtc gcaagtaacg ccaaatagcg cccagacaaa gaggatgccc caataccccg
    16021 gcattgtcgt caaataaaca cccagtaatc ctgtcgcaat gagtgagaat gacatggtgt
    16081 atctgcgcgg tagtttatcg gcaaaataaa tagacagaaa aaagcccact gtggtgacaa
    16141 atgagtttac cgacatagca ttaccaattt gaccattggt caaatggaaa tattcctgca
    16201 tcgggatata aaacgcatct ttcagcgacg gtaatttata aattgtaccg ccacacagtg
    16261 ttaatagact aaataacgcc catttctttt tcgtgagcat aacatcccct aagcgatttt
    16321 taaaatcact gttttaaagg caataagata ccgaccattc atatagaatg gttttattaa
    16381 ctgcgtatga gttaaaactt attagtggtt cagaagatgg tttgcctctt gtttgatttg
    16441 ttgtagtacg ttacgcacat gatttatctg attaattgca tcttcttttt ctttacgtaa
    16501 ccgcgcatcc agtaaatgat tttctggtag gggtgtttca ctcatctggc gataagggat
    16561 ccacggatta tcatcttcgt cttcaaagcg gaacgccggc gcatagtaat caacctcttc
    16621 ttccaggcca tatgccgtca cctgcggctc atcatcacca aggcagataa ggtgcgttaa
    16681 taacgctttg aacggcatat caccctgacc tgaaatacag gctttatgac ccaggccacc
    16741 ctgttcttta acgatcaagg catctttaat atggacctgg gtaatatgcg gtgccatcgt
    16801 ttttaaggcg tcgatgggat gctcatttgc attgatcatg ttcgcaaaat caaacagtaa
    16861 ggaaagagac tccatctcac tttctttgac cagcgacacc aactcatgac tttttaaatc
    16921 ttcatgctgc tcgatcgtaa aagtcaggcc gctgtcctga tacgtttccc gtacataggc
    16981 aatgtcgtta gcgataatcg ataatacgtc gcgcaaatta ccttcataac gtggataaaa
    17041 acgtacggac gatgccccag ttttcaacgc gatggcgacg gcttcgtcaa tagatgcctt
    17101 atctgaggcg ctggtttcaa tatgaatatc aaggttcaga cggcgggctt tatctccaaa
    17161 ggcagagagt tctttatcgt ccatattacc aagagaaaaa cgctcgccat ccagaacatg
    17221 gattttgacg ccacgtagat tattttcact tgcgatatca agtaaatcag caggcaacac
    17281 tctttccagc cgcatattta aatgaaaggc ataagcatgt aaataaagag gaagagtgtc
    17341 gacacgttgt agaatctttc ttgcgttatt tattgtcatc ataaaacacc tacagattaa
    17401 tgatgaggtc tacggcctca aatcgccaat attcaatatc tatctggaca atattgcctt
    17461 gctggtcggc gcgatgcttt tcaattaaga tcgctggcat acccgcagaa ccgccaagat
    17521 acttgctgat atcgcccggc attctgacgg gtttgaaaat caattttttt actaccgtct
    17581 ctttttggta gcacttttcc cagactgcag aaaatgagtg gttttcaagt tgttcaataa
    17641 aacccggagc aacttcagga ttaatatatg tttcgtgata gaaaacttta tgtccttcca
    17701 gcgcccccca gccggtgatg cgatagagtt cagttgatgg tgtcactgca atcagtggcg
    17761 cgagcgtctc gggaatatcg ctggtacggc ttttctcggt aaacccccag gaaggttccc
    17821 gcccttgctc aattgcagcc cgctgaaagc tcgccgacag ctctggactg taattaaatc
    17881 gtggctgggt cacgaaccac cctttacgat ccttacggaa gattttggat tctgcctcga
    17941 ggttcaacaa cgcctggcgc agcgtcatac gtttaatgcc cagcagttct cccagctctc
    18001 tttctgaagg gagttttcca cccggtgtgg tgatgccatc tttcaaccac cgatccagtt
    18061 tctcttttgc attttctacg gtggattgat tctccgccat tgtttaatcc ttttggttta
    18121 aaccagatga agcatcttac gcttacgcca aaaaagaaag caattaacgg cgtatgactt
    18181 tgcagataat tgtgacaaag ctctctattg acgtaatcac agacttataa gggagatact
    18241 cgcattggtg tgcgctcacg acaggtaaaa aaaaaacctg ccagcgatgg caggtttttt
    18301 ataactaaaa aacgaaatta atcgtctttc ggtgcgcggt tggcgcggcg acgatcgttt
    18361 tccgtcaggt gacgtttacg aatacggata gaggtcggag tcacttctac cagttcgtca
    18421 tcatcgatga actccagagc ttgttccaga gtcatgcgga taggcggaac cagaacaacg
    18481 gcttcgtcag taccggaagc acgcatgttg gtcagtttct taccggtcag gcagtttaca
    18541 gtcaagtcgt tagagcggct atgaataccg ataatctgac cttcgtaaac ttctgcaccg
    18601 tgaccgagga acagcttacc gcgatcctgc agaccaaaca gcgcgaacgc gaccgcttta
    18661 ccctgaccgt tagagatcag tacaccgttc tgacgctgac ccacttcgcc cggacgtacg
    18721 tcgtcgtagt ggctgaaggt ggagtacagc agaccagtac cggaagtcat ggtcatgaac
    18781 tcagaacgga agccaatcag accacggctt gggatcacgt agtcgagacg tacgcggcct
    18841 ttaccgtctg gattcatgtt tttcaggtcg cctttacgtt cgcccagcgc ctgcattacg
    18901 gaaccctgat gctgttcttc aacgtccaga gtcacgtttt catacggctc ttgtttacga
    18961 ccatcgattt cacggaagat tactttcgga cgggataccg ccagttcgaa accttcacga
    19021 cgcatgtttt cgataagaac agacaggtgc agttcgccac gaccagaaac gcggaacgca
    19081 tcggcgtctt cggtttcttc tacgcgcagc gcaacgttgt gtaccagttc tttgttcaga
    19141 cgatccagga tctgacgaga cgttacgaac ttaccttctt taccgcagaa cggcgaggtg
    19201 ttaacgcaga agaacataga aacggtcggc tcatcaacgg agagtgccgg cagcgcttca
    19261 acgttttgcg tgtcgcaaac ggtgtcagaa atgttcagtt cgccaagacc cgtgatcgcc
    19321 acgatatcgc cagcttccgc cagatcggtt tcgatacgtt ccagaccgag gtggcccagc
    19381 actttaccga ctttcgcgtt gcgggttttg ccttcgctat cgatgatagt gacctgctgg
    19441 ttcggcttca ctttaccgcg cttgatgcgg ccaatgccga taacgccaac atagctgttg
    19501 taatcgagct gagaaatctg catctggaac ggaccgtcaa ggtcaacgtc cggcgcagga
    19561 acgtgatcaa caatcgcctg atacagcggg gtcatgtctt ccgccatatc ttcgtggtcc
    19621 agacccgcga taccgttcag cgcagaagcg taaacgatcg ggaagtccag ctgctcgtcg
    19681 gtcgcgtcga ggttaacgaa cagatcgaat acctgatcca caacccaatc aggacgcgcg
    19741 ccagggcggt caactttgtt gataacaaca atcggcttca ggccgtaagc aaacgctttt
    19801 ttggttacga agcgcgtttg cggcatcggg ccgtcaaatg cgtcaaccac cagcagcact
    19861 gagtctacca tggacattac acgttcaact tcaccaccga agtcggcgtg ccccggggta
    19921 tcaacgatgt tgatacggta atcattccat ttgatagcgg tgtttttcgc gaggatggta
    19981 atcccacgct ctttctccaa atcgttggag tccatcacgc gctcttgggt ttcggcacga
    20041 gagtcgaacg taccggattg ttggagcagc ttgtctacca gggtggtttt accatggtct
    20101 acgtgcgcga tgatggcgat attacgcaat ttttcgatca caactttgcc tcaggcatta
    20161 gaaatagcgc gttattgtac acggattaat cgcactacaa aacaggatca caaacatcct
    20221 ccgcaaacaa gtattgcaga gtccctttgt gatcgctttc acggagcata aaaagggtta
    20281 tccaaaggtc attgcaccaa tatggtgctt aatgtttcca ttgaagcact atattggtgc
    20341 aacattcaca tcatggtgca gcccttttgc acgatgttaa gcatgataac gccttttagg
    20401 ggcaatttaa aagttggcac agttttcgct ttatcttttt tacggcgaca cggccaaaat
    20461 aattgcagat ttcgttacca cgacgacaat gaccaatcca ggagagttaa agtatgtccg
    20521 ctgaacacgt actgacgatg ctgaacgagc acgaagtgaa gtttgttgat ttgcgcttca
    20581 ccgataccaa aggtaaagaa cagcacgtca ctatccctgc tcatcaggtg aatgctgaat
    20641 tcttcgaaga aggcaaaatg tttgacggct cctcgattgg cggctggaaa ggcattaacg
    20701 aatccgacat ggtgctgatg ccagacgcat ccaccgcagt gattgacccg ttcttcgccg
    20761 actccaccct gattatccgt tgcgacatcc ttgaacctgg caccctgcaa ggctatgacc
    20821 gtgacccgcg ctctattgcg aagcgcgccg aagattacct gcgttccact ggcattgccg
    20881 acaccgtact gttcgggcca gaacctgaat tcttcctgtt cgatgacatc cgtttcggat
    20941 catctatctc cggttctcat gttgctatcg acgatatcga aggcgcatgg aactcctcca
    21001 cccaatatga aggtggtaac aaaggtcacc gtccggcagt gaaaggcggt tacttcccgg
    21061 ttccaccggt agactcggct caggatattc gttctgaaat gtgtctggtg atggaacaga
    21121 tgggtctggt ggttgaagcc catcaccacg aagtagcgac tgctggtcag aacgaagtgg
    21181 ctacccgctt caataccatg accaaaaaag ctgacgaaat tcagatctac aaatatgttg
    21241 tgcacaacgt agcgcaccgc ttcggtaaaa ctgcgacctt tatgccaaaa ccgatgttcg
    21301 gtgataacgg ctccggtatg cactgccaca tgtcgctgtc taaaaacggc gttaacctgt
    21361 tcgcgggcga caaatacgca ggtctgtctg agcaggcgct gtactacatt ggcggcgtaa
    21421 tcaaacacgc taaagcgatt aacgccctgg caaacccgac caccaactct tataagcgtc
    21481 tggtcccggg ctacgaagca ccggtaatgc tggcttactc tgcacgcaac cgttctgcgt
    21541 ctatccgtat tccggtggtt tcttctccga aagcacgtcg tatcgaagta cgtttcccgg
    21601 atccggcggc taacccgtac ctgtgctttg ctgccctgct gatggccggt cttgatggca
    21661 tcaagaacaa gatccatccg ggcgaagcca tggacaaaaa cctgtatgac ctgccgccag
    21721 aagaagcgaa agagatccca caagttgcag gctctctgga agaagcactg aacgagctgg
    21781 atctggaccg cgagttcctg aaagccggtg gcgtgttcac ggatgaagcg atcgatgcgt
    21841 acatcgctct gcgtcgcgaa gaagatgacc gcgtgcgtat gactccgcat ccggtagagt
    21901 ttgagctgta ctacagcgtc taatagttga agttgtacta cccggcgcaa caacgccggg
    21961 atttagttgc cgtggaaact ttcagcccat ctctgcatgg gcttttttct ccgtcaattc
    22021 tctgatgctt cgcgcttttt atccgtaaaa agctataatg cactaaaatg gtgcaacctg
    22081 tttaggagac tgctttatgg caacaggcac gcagcccgat gctgggcaga tcctcaactc
    22141 gctgattaac agtattttgt taatcgatga caacctggcg atccattacg ccaaccccgc
    22201 cgcgcaacaa ctgctcgccc aaagctcccg caaattgttt ggtacgccgt taccggaact
    22261 gttgagctac ttctcattaa atatcgagct gatgcaagaa agtctggagg cggggcaagg
    22321 ttttaccgat aacgaagtga cgctggtgat cgacggtcgc tcgcatatcc tttctgtgac
    22381 ggcccagcgt atgccggacg gcatgatcct gctggagatg gcgccgatgg ataaccagcg
    22441 ccgcttaagc caggaacagc tacaacatgc tcaacaagtt gctgcccgtg atttagtgcg
    22501 tggcctggca catgagatta aaaatccgct tggcggttta cgtggcgcgg cgcagctact
    22561 cagcaaagca ttacctgacc cgtcactgct ggaatatacc aaagtgatta tcgaacaggc
    22621 ggaccggctg cgaaatctgg tcgaccgtct gttggggccg cagctgcccg gtacgcgcgt
    22681 gaccgaaagt attcacaaag tagctgaacg cgtggtgacg ctggtgtcga tggaactgcc
    22741 ggacaacgtg cggttgattc gtgattacga tcccagcctg ccggaactgg cgcacgaccc
    22801 ggatcaaatt gaacaggtct tgctgaatat tgtgcgcaat gcgctacagg cgctggggcc
    22861 ggaaggcggt gaaatcattc tgcgtacccg caccgcgttt caactgacct tacacggcga
    22921 acgctatcgg ctggcggcgc ggattgatgt ggaagataac gggccaggca ttccgcctca
    22981 tttgcaggat acgctgtttt acccgatggt cagcggccgc gaaggtggca ccgggcttgg
    23041 cttatccatc gctcgtaatt tgattgatca gcattcaggc aaaattgaat ttaccagttg
    23101 gccagggcat accgagttct cggtttacct gcctatcagg aaataaaggt gacgtttatg
    23161 caacgaggga tagtctgggt agtcgatgac gatagttcca tccgttgggt gcttgaacgt
    23221 gcgctcgctg gagcgggttt aacctgtacg acatttgaga acggcgcgga ggtactggag
    23281 gcgctggcga gcaaaacgcc ggatgtgctg ctttcagata tccgtatgcc gggaatggac
    23341 gggctggcgc tgctcaagca gattaaacag cgccatccga tgcttccggt catcattatg
    23401 accgcacatt ccgatctgga tgctgccgtc agcgcctatc aacaaggggc gttcgattat
    23461 ctgcccaaac cgtttgatat cgacgaagcc gtggcgctgg ttgagcgcgc catcagccat
    23521 tatcaggaac agcagcagcc gcgtaatatt cagcttaacg gcccaacgac cgatatcatc
    23581 ggcgaagcgc cagccatgca ggacgtgttc cgtattatcg gtcggctttc gcgttcttct
    23641 attagcgtgc tgattaacgg cgaatccggc accggtaaag aactggtcgc tcatgccctg
    23701 catcgccaca gtccgcgagc caaagcgccg tttatcgcgc tcaatatggc ggctatcccg
    23761 aaggatttga tcgaatccga actgtttggt cacgagaaag gcgcatttac cggcgcgaat
    23821 accattcgtc aggggcgttt tgaacaggct gatggcggta cattattcct cgatgaaatt
    23881 ggcgatatgc cgctggatgt gcagacgcgt ttgctgcgcg tgctggcaga tggtcagttt
    23941 taccgcgttg gcggctatgc gccggtgaaa gtggatgtgc ggattatcgc tgccactcac
    24001 cagaatctcg aacagcgggt gcaggaaggt aagttccgtg aggatctgtt ccaccgcctg
    24061 aacgttatcc gcgttcatct gccgccgctg cgcgaacgtc gggaagatat tccccgtctg
    24121 gcgcgccatt ttttacaggt tgccgcgcgc gaactgggcg tagaagcgaa gttgctgcat
    24181 ccggaaaccg aagctgctct gacgcgtctg gcgtggccag gcaacgtgcg ccagctggaa
    24241 aacacctgcc gctggctaac ggtgatggcc gccgggcagg aagtgttgat tcaggatttg
    24301 cccggcgaac tgtttgaatc aacggttgcg gagagtactt cgcaaatgca accggacagt
    24361 tgggcaacgc tgttagcaca gtgggcagat cgggcgctac gttccggtca tcaaaatctg
    24421 ctttccgaag cgcagccaga gctggagcgg acgttactga cgaccgcgtt gcgacatacg
    24481 caggggcata aacaggaagc ggcgcggcta ctcggctggg gccgcaacac tctgacgcgt
    24541 aagttaaaag agctggggat ggagtgattc acggcttgtg tgtaaagatt gattattgag
    24601 cgcaaattgc tggtattttg cgctttactg ttccgataag ttcagtatga tcttgcccgg
    24661 aaaacgggga gagtcattat gctggaatca ataattaatc tggtatcgag tggcgcagtt
    24721 gacagccaca caccgcaaac tgccgttgct gccgtgctgt gtgccgcgat gattgggctg
    24781 tttagctgaa gctcgttgtt tatgccggat gcggagaaag tcgtaggcct gataagacgc
    24841 gaccagcgtc gcatcaggca tcatgctcag atgcctgatg cggcgtaaac gccttatccg
    24901 gcctacaaaa gcaataattt caacaggttt tgttgcccag cgcatcaggc aatcttgttc
    24961 tcagcacatt gatggcgctt tcgttttcta ctttgtaaac gaagcgccat tcactattca
    25021 aatcacccgt gagaactgct gcatccgcgc tttctggcgc agataggtat caaagcacat
    25081 gcaaatgttg cggatcagca agcgaccttt cgccgtcacc tgtattccct tctcatccac
    25141 atccaccagc ccatcttttg ctaacggggc gagcagcttg agatcttccg caaagtaatc
    25201 agcgaagtgc aaatcccact gtttctcaat aggggcgtaa tccagacgga agttgcagat
    25261 gagcgactta atcacatcgc ggcgaataca gtcatcacgc gttagcgcaa taccacgcca
    25321 cagcgcattg ccttgttcat ccacttgctg atagtactgc ttcaactctt tctggttctg
    25381 cgcgtagcag tcgccaatca tgctgatggc ggaaacgccc atccccagca gatcggtatc
    25441 gccctgagtg gtgtagccct ggaaattacg atgcagcacg ccttcacgct gggccaccgc
    25501 cagctcgtca tccggacggg caaagtgatc cataccgata aactgatagc ccgattgcgt
    25561 caggaaggcg atggtttcct gcaggatatc gagtttttgc tgcggactcg gcaggtcagc
    25621 atctttgatt ttgcgctgag cagcaaaaat ggtcggcaga tgcgcatagt taaagacgct
    25681 cagacggtca gggtttagct ccgccacgcg ttttagggta aaggcgaaac tttccggcgt
    25741 ctgtttcggc aggccgtaaa tcaggtcgat gttggtggag gtaaaaccaa tctcacgcgc
    25801 atggttaagc agtgcaaaga tgaactcttc atcctgctcg cggttaacca gacgttgcac
    25861 ttctttgttg aagtcctgca cgcccatgct caggcgatta aagccttcgg cgcgtaaatg
    25921 atcgagtaca tccagttcga tttcccgcgg atcgacttcg atcgaaatct ccgcatcggt
    25981 attgaaatgg aagttttcgc gcagcagctt catcagacgg ctgatttgcg ctttattcag
    26041 atacgtcggc gttccgccgc cccagtgcaa ttggctgacg tgacgcccgg cgaacagcgg
    26101 tgcacgatgg acgatttctt gctccagcgc gtccagatac tgatcggcct tgtgctgctg
    26161 gcgagtaaca atcttattgc aaccgcagaa gtaacaaagc ttatggcaga acgggatatg
    26221 tacgtagaga gataatggac gctcaggata gcgcgccacg gcttgtaaaa acgcctgttc
    26281 gccgaagtct tctgaaaact ccagcgcggt cgggtacgag gtgtatcgtg gcccggaata
    26341 gttatatttc tggatcaggg ccagatccca gtcgatttgc tgtacagaca tgctcactcc
    26401 ttccgatggc gtctctggcg gctacggcgt accggcggat gcccgtttcg ggtcatcagc
    26461 cggttgcgca gccacttttg tcgccgcgac aaccgctgta gtttaacgaa taaccacacc
    26521 agataacata taaccggaag ggttatcagc agaacgggaa ggcccacggc ggcaatccgt
    26581 tagttgcccc gcagcagacg catcatgtct tcttgcttct cgtcttcttc ctcttcttcg
    26641 tcatcgtcat aagagaggcc gagtttctgc atcaactcat caatacgatc cagtttagca
    26701 tccacccagg attgctcttc ggcgctcagg gtttcgcctg cttccagacg ttccagcagc
    26761 gcatccagac gctcatccgt ttccagtaat tccaactccg cctgcggtga aagcataggt
    26821 ttctcactct tcggtttgtg ctgtttggtg actttttcag tcactcccaa tggaataggg
    26881 gttttactgc caatacgtgg atcttttggt gcgttctggc ctttgctgcc tgacgtggtg
    26941 tttccgcccg ctgcgcggct gcccggcgca tgaccacgac gttttttctg acgcttgcgg
    27001 tcacgagctt cctgattcag ctcttcgcgc gttttgcgac gcgcttttgc atgacctttg
    27061 ctgcgtgatg gtgaagatgg tttcataatg gttcgtcttt tagccgtttt attcagtata
    27121 gatttgcggc ggaatctaac agaaagcaag caaagaaaaa aggcgacaga ttactctgtc
    27181 gccttttttc ctgactcata acccttaacg ggtcttacaa tccttgcagg ccgccaacac
    27241 accctgtttt tccattagcc ggaacaatcc gtgttgattc catttccgtt taattacgtc
    27301 tccggacgtt tgtcttcctg acaatcctct gtcttcgcct cctggcgctc ctgaccctca
    27361 tcctgagtca ttgttcctgt tagcgtcctc gctctatggg tgttacttta cgcctttgct
    27421 ttaaacaaac aatcaaccgc catcagtatt tgcacatttt cagagtttta tataaaagca
    27481 attcactgat ttatatagct tatttgacat gcatcgcaga attttctctg ctatttcgca
    27541 taaagactat cggcaatgtc tcacaaagcg tatggctttt acttacaacg ctcacgccga
    27601 aaaatccgcc ttttattatg gttcaggtat aatcgccgca aagctttgac tgacggagac
    27661 gaccgctttg actaatttga attatcaaca gacgcatttt gtgatgagtg cgcctgatat
    27721 tcgccaccta ccttccgata ccggaattga agtggctttt gcaggccgtt ccaacgcagg
    27781 taaatccagc gcgctgaaca cgctgactaa ccagaaaagc ctggctcgta cctcaaaaac
    27841 ccctggacgc acccagctta tcaacctgtt tgaagtggct gacggcaagc gtctggttga
    27901 cttgcctggg tacggttatg cggaagtccc ggaagagatg aagcgcaaat ggcagcgcgc
    27961 gctcggcgag tacctcgaaa aacgtcagag cctgcaaggt ctggtggtgc taatggatat
    28021 tcgccatccg ctgaaagatt tggatcagca gatgattgag tgggcggtag acagcaatat
    28081 cgccgtgctg gtactgctga ccaaagcgga caaactggca agcggcgcac gtaaagcgca
    28141 attgaatatg gtgcgtgaag ctgtactggc gtttaacggt gatgtgcagg ttgaaacgtt
    28201 ttcttcgttg aagaaacaag gcgtggacaa gctgcggcag aaactggata cctggtttag
    28261 cgagatgcag cctgtagaag aaacgcagga cggcgaataa ttttcttgcc ttaatgcttg
    28321 tgccggatgt ggcgtatccg gcccgtaaat tcaatagatt acaattctac cgttggcctg
    28381 ataaacatag cgcatcaggc attacggatc ttttctttcg cccaataaaa aacgccccag
    28441 tcattactga ctggggcggc taaaatattc agccaaatcc gattacgtga agtaaaaggt
    28501 ctgaaagata gaacatctta cctctgtacc ctacgcgact aactttactc ttttttgttc
    28561 agttcagaaa gcactttttg taattttttt tcagttgttg cataggaatt tctaatagcc
    28621 atcacaaaac gtgacagctt atgttgctta cttacgaaaa aagggcatgt tcaggcgaat
    28681 cttagtgcgc ctgatcccag ttttcgccac tccccacttc caccagcaac ggcacatcca
    28741 gacgggtaca gttttccatc agttgatgaa tctgcttcgc gacggcatca acatcatctt
    28801 tatgaacttc aaataccagt tcatcgtgta cctgcatgat catacgtaca cgcggttgct
    28861 cagcctgtaa ccacgcatca acggcaatca tcgcccgttt gataatgtcg gcggcggttc
    28921 cctgcattgg cgcgttaatg gctgcacgtt cagccgctgc acgacgagca ccattgctgg
    28981 atttgatatc cggcagatac agacggcgtc cgtccagcgt ttcaacgtag ccctgctctt
    29041 tcgcctgagc acgggtgcgt tccatatact ccagcacgcc agggtagcgt tcgaagtaaa
    29101 ggtccatgta cttctgcgct tctttacgtg gaatgttcaa ttgccgcgcc agaccgaaag
    29161 cactcatgcc ataaatcaga ccaaagttga tcgctttcgc gctacggcgt tgctcgctgg
    29221 tgacggtttc cagtggcaaa ccaaacactt ctgccgccgt tgcccggtgg atatcttttc
    29281 cttccgcgaa tgcggtcagc aagcctttgt cacgcgagag atgcgccata atgcgcagtt
    29341 caatctgcga gtagtccgct gagacaatca cataatcctc tggcgcaata aacgcctggc
    29401 ggatacggcg accttcttcg ttacgtaccg gaatgttttg caggttagga tcggttgacg
    29461 ataaacgtcc cgttgcagtt actgcctggt gataagaggt atgcacacgc ccggttttcg
    29521 ggttgatcat cagcggcagc ttgtcggtgt aggtcgattt cagcttcgcc agaccacgat
    29581 actccagaat cacttttggc aacggatagt ccagcgccag ttcttccagt acctcttccg
    29641 acgttgacgg cgcgccaccc ggcgttttct tcagcggttt aatgccctgt ttttcaaaca
    29701 gaatggtttg taactgcttg gtggaagaaa ggttaaattc ctcacctgca atttcatgcg
    29761 ctttcttttc cagctcagcc agacgcaagg taagctcttc agaatgattg tgcagcactt
    29821 tcggatcgat cttcacaccg ttacgttcaa tgcgtgaaag caccggcacc agcggcattt
    29881 cgatattctc gaagacgttc aacggccctt tgtgtttttg cagatctggc cacattttca
    29941 gatgcaactg caaggtgaca tccgcatctt cggcggcgta acgtccggcc tcttcgaggg
    30001 caatctggtt aaaggtcagt tgatttttgc ctttacccgc aatctcttca aaagtgatgg
    30061 ttttgtgctt caaccaacgt tccgcgaggc tgtccatatc gtgacgcccg gcaacgctat
    30121 tgagaatgta ggactccagc atggtatcaa acgcaatccc acgcagctca atgccgtaat
    30181 tcgccagaat accgcgatcg tatttcaggt tttgcccgac cttcagcgcc ttttcatctt
    30241 ccagcagcgg ttttagcaac tcgagtgcac gctcgcgaga gatttgatcg ggcgcatcaa
    30301 gataatcatg agcaaccgga atatatgccg ctacgcctgg ctcgatagca aaagaaagcc
    30361 cgaccaggtt agcagagatg ttatcaaggc tgtcggtttc ggtatcaaat gcaaataccg
    30421 gcgctttttc cagcttcgca atccacgctt tcagtgtttc ttcatcaagg atggtgacgt
    30481 agttgtcata agaaatcacc gttgccgtca cttctggtgc ttcgtctgca acactggttt
    30541 cctgtggctt cgcggcgggt tttgcccctt tggcctgtaa ccatttgccc gcttcgacat
    30601 cagcagtcca gcgtttgaac tcatactttt tgaacagccc caacaactct tccgctgccg
    30661 gttgctgcac ttccagttgt tcacaggtca gctccagttc aacgtcggtt ttaatcgtcg
    30721 ccagctggta tgagagataa gcaacttctt tgttttgctc gagcttcgct gccattgttt
    30781 tcgcgccacg gaagctcaac ccagcaattt tttctggctc ggcatacagc gtatccagcc
    30841 cgccaagacc ttgcagcaat gcctgcgcgg ttttttcacc gacgcccggt acgccaggaa
    30901 tgttatcaga ggagtcaccc atcagcgcca ggaaatcgat gatcagttct ggcggcacgc
    30961 cgtacttatt caccacctct tccggtccga ggatggtatt cgtcatggta ttgataagcg
    31021 taatatttgg cgtcaccagc tgcgccatat ctttatcgcc agtgctgatc agcaccggac
    31081 gcccggcttt ttcggcttcg cgcgccagag taccgataac gtcgtccgct tctacgccag
    31141 aaaccgccag cagcggcagt cccatcgctt taaccatcgc gtgcaagggt tcgatttgtg
    31201 cacgcagatc gtccggcatt ggcgggcgat gtgatttgta atgttcaaac agttcatcac
    31261 gaaaggtttt tcccttggcg tcaaagacca ccgctgcatg cgtcggttta tattgcatga
    31321 tcagactgcg cagcatgttg aggacaccat acatcgcacc ggtcggctcg cctgcgctgt
    31381 tagtcagcgg aggaaacgcg tgatatgcgc gataaagata agatgaacca tctacaagga
    31441 taagtggatt ttgggggatc tgaaccataa tgtccgtgcc tgtttatcag attatgggta
    31501 aaggatgcca cagacaggat aaaaacatga gtttttcacc gcttttcgtg aaaatctttt
    31561 gtagatcttc tggatcgctc gcaaatcgtc atgtggataa ctttgtgcat aaattattta
    31621 catcgctttt aatttattat catgcttttt acagcatgat gtttgtcttt gtttttcatg
    31681 cagttaaaag taaagccatc cctgataatt gagataatgg gattttttct aatgtggata
    31741 gatatgaatt atttttctcc ttaaggatca tccgttattt gggtcgtttt ctgctaaaat
    31801 ctgcgccttg cgcttcttta gcgcactgtc tatggctaac cttttgaata aattcattat
    31861 gacgagaata ctcgctgcga taaccctttt gctgagtatc gtattgacca ttttggtcac
    31921 tattttttgt tccgtcccga tcatcattgc cgggattgta aaactattgc tacctgtgcc
    31981 agtcatttgg cgaaaggttt cgcgtttttg tgattttatg atgtattgct ggtgtgaagg
    32041 tctggcggta ttactgcatc tgaacccaca cctgcaatgg gaagttcacg ggctggaagg
    32101 gttaagtaag aagaactggt atctgcttat ttgtaaccac cgtagttggg cagatattgt
    32161 cgtactgtgc gtgctgtttc gtaagcacat tccgatgaat aaatatttcc tcaagcaaca
    32221 gctggcctgg gtgccatttc ttggcctggc gtgctgggcg ctggatatgc cgtttatgaa
    32281 gcgttattcc cgcgcttatt tattacgtca tccggagcga cgtggtaaag atgttgaaac
    32341 cactcggcgt tcttgtgaaa agtttcgcct gcatcccacc accattgtca atttcgttga
    32401 aggctcccgt ttcacgcaag aaaaacatca gcaaacccac tccacttttc aaaacttgtt
    32461 gccaccaaag gcggcaggca ttgcgatggc gctaaatgta ctgggtaaac aattcgataa
    32521 actgcttaat gttacgttgt gttatccgga caataaccgt cagccgttct tcgatatgtt
    32581 aagcggtaaa ctgacgcgga ttgtcgtaca tgtggattta caacctattg ccgatgagtt
    32641 acatggcgac tacatcaatg acaaaagctt taagcgccat tttcagcaat ggctgaactc
    32701 actgtggcag gaaaaagacc ggcttctgac gtcgttgatg tcatcacagc ggcaggacaa
    32761 gtaaattttg ctcgcctctg aatgagcaga ggcgagtgag tattttaatg aacaaaacgt
    32821 ccggcacgag acataaattc ttcttcgctc atctcctgtc cgttaaaaac aactttaccc
    32881 ggtgtataac gcagttgcag cgaggcggta ttgttgtcaa tcgtgattaa ctgaaacatc
    32941 tgacctaatg tcatcatccc gctgatttgt ttatccgcct gcttttgcgc tttttccgca
    33001 tccattcctt cagataaatt aagctgtttt gcggtttctg tgacgacatt aagcggtaac
    33061 ttcacatcaa aattgagcga tttgatatct ttgtttgtgg atgatgaaga tttggctggg
    33121 tcggcaatac tgatatccag attggcattt agttcgccga gtgcgttctt ccatcttacc
    33181 ggttgtttaa tggtcggctc acttttttgt aataacggta aatactcttt gaacaatgca
    33241 gcattcactt cttgcagagc aacttcatcg tttgctaact cagggtgtgc agcaagctgc
    33301 ttctgcatcg caatgttata ctggatgata aattggcgca ctgctgaggg atcaatagat
    33361 tcagcaatca atgaccactc accactaccg agttgctgat tcccttttgt taacttatca
    33421 agttcgtagg tcagattgac attgatataa tctttgtctt taacgcgatc cagcctggtt
    33481 tttgcaacga aagcatcgat ttgggcaacg tcttccccgt gattgataat gttaatctga
    33541 ttaaatttcg actcgctttc accaaccgga aatttctttt cttctaatcg cgccagagaa
    33601 tcaatggtaa aactttttgc tgttactttg ttcattggtg ataattgcag aacaatatct
    33661 tcgactttgc cttcaatatc caggttagac aatgaactac catcaccatt aaaagtaaac
    33721 tggccctcac caaaagaaaa ctttccatac tctgcagggg caacatctaa tgtcgtagag
    33781 aatgagccac caaacgcgaa tcgcaatgtc gcttccacgg gcgatttatt tttcgcggcg
    33841 ataaatagcg gttgtgtcag ttcattattc actaagttca cttttgccgc tgccagtgct
    33901 gggatgacat taccatgcat taacattgtg atgggcaacg gaccgtgctc aacatccaca
    33961 tcaaatgcaa ctttctggcc tggcttgata ttgagatcgg gtgcaccgtt atcaaatgaa
    34021 atttgcattt gaaaatggga attaaaaaag cctttgtcaa agtttttata acttaacgtc
    34081 atatcgtagg catgttcacc tttcttttta gcatcgttaa aatctttaat aaatttttcg
    34141 acaccaggct gaatttgcgt acctgtgtac catgtgccac caccccagat tacggccaac
    34201 gcaacaataa cacctgtagc tgacttacgt atcatataaa tattccctga taaaacagca
    34261 aaactttgaa tatccactta tgctga
//
LOCUS       DAPGOF010000035        34025 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.035, whole genome shotgun sequence.
ACCESSION   DAPGOF010000035 DAPGOF010000000
VERSION     DAPGOF010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34025
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.035"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            257..2962
                     /gene="malT"
                     /locus_tag="RHV13_001447"
     CDS             257..2962
                     /gene="malT"
                     /locus_tag="RHV13_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HDV1241000.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREDTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQDAVQHAQQLLKMMGYGV"
     gene            complement(3005..4021)
                     /gene="rtcA"
                     /locus_tag="RHV13_001448"
     CDS             complement(3005..4021)
                     /gene="rtcA"
                     /locus_tag="RHV13_001448"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HDV1241001.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAATEICGATVEGAELGSQRLLFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     TAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFSLIETDGVTR
                     VSIE"
     gene            complement(4025..5251)
                     /gene="rtcB"
                     /locus_tag="RHV13_001449"
     CDS             complement(4025..5251)
                     /gene="rtcB"
                     /locus_tag="RHV13_001449"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HDV1241002.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAEDLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            5440..7038
                     /gene="rtcR"
                     /locus_tag="RHV13_001450"
     CDS             5440..7038
                     /gene="rtcR"
                     /locus_tag="RHV13_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HDV1241003.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVSVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(7020..7778)
                     /locus_tag="RHV13_001451"
     CDS             complement(7020..7778)
                     /locus_tag="RHV13_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HDV1241004.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(7795..8625)
                     /gene="glpG"
                     /locus_tag="RHV13_001452"
     CDS             complement(7795..8625)
                     /gene="glpG"
                     /locus_tag="RHV13_001452"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HDV1241005.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENTADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(8670..8996)
                     /gene="glpE"
                     /locus_tag="RHV13_001453"
     CDS             complement(8670..8996)
                     /gene="glpE"
                     /locus_tag="RHV13_001453"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HDV1241006.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            9186..10691
                     /gene="glpD"
                     /locus_tag="RHV13_001454"
     CDS             9186..10691
                     /gene="glpD"
                     /locus_tag="RHV13_001454"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HDV1241007.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLNVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAVSDLGEDFGHEFYETE
                     LKYLVDHEWVRRADDALWRRTKQGMWLSAEQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(10906..12933)
                     /locus_tag="RHV13_001455"
     CDS             complement(10906..12933)
                     /locus_tag="RHV13_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001718984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDV1241008.1"
                     /translation="MQNRKWILSSLVMTFFGIPILTQFLAAVVAMLGVGLAGIIEVCN
                     ILITPTIYLLLNVFMLTLGAIIIFFSGRVWDGDSAPENREIAVWRQCFFLLPALLTLV
                     GWIIALHLADYQFRQMGAGWLANLMLPWLGVFLVSLVGGEYWWMVIIPVGAHISFSLG
                     YAWPTRYPLSGTSGLRCRNLLLFLLLLLGIVAGYQAHLYKQQNPGVGVRENIDTWAWR
                     PDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHTREYLEN
                     SRTPDAYNRIVKGDADIIFVAQPSGRQKKRAEESGVTLLYTPFAREAFVFIVNADNPV
                     NSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISPQ
                     ETEVASVMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINDITPTAENIR
                     NGKYAYIVDAFMVTRENATPETQKFVDWFLSPQGQQLVEDVGYVPLLVWPTVVALAAC
                     IITLSFLGEMLCSISLMIRRISLLQLQLGLLAEYTLLFCALCCVGWQFARITQSELKS
                     KLHYWLVFVPVYFWLLLWNIIFYVAPAKIALLESIRNFFLLVVWLPLNFSPFWPQPWT
                     DFIGPISAQLGFALGYYCQWRSKNRSQIRKWSEWGTCLSFVTLGMMAWALR"
     gene            complement(13062..15509)
                     /gene="glgP"
                     /locus_tag="RHV13_001456"
     CDS             complement(13062..15509)
                     /gene="glgP"
                     /locus_tag="RHV13_001456"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HDV1241009.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(15528..16961)
                     /gene="glgA"
                     /locus_tag="RHV13_001457"
     CDS             complement(15528..16961)
                     /gene="glgA"
                     /locus_tag="RHV13_001457"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HDV1241010.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(16961..18256)
                     /gene="glgC"
                     /locus_tag="RHV13_001458"
     CDS             complement(16961..18256)
                     /gene="glgC"
                     /locus_tag="RHV13_001458"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HDV1241011.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(18274..20247)
                     /gene="glgX"
                     /locus_tag="RHV13_001459"
     CDS             complement(18274..20247)
                     /gene="glgX"
                     /locus_tag="RHV13_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HDV1241012.1"
                     /translation="MTRLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIEG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYACACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(20244..22430)
                     /gene="glgB"
                     /locus_tag="RHV13_001460"
     CDS             complement(20244..22430)
                     /gene="glgB"
                     /locus_tag="RHV13_001460"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HDV1241013.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(22703..23806)
                     /gene="asd"
                     /locus_tag="RHV13_001461"
     CDS             complement(22703..23806)
                     /gene="asd"
                     /locus_tag="RHV13_001461"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDV1241014.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            23998..24591
                     /gene="yhgN"
                     /locus_tag="RHV13_001462"
     CDS             23998..24591
                     /gene="yhgN"
                     /locus_tag="RHV13_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HDV1241015.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(24637..25932)
                     /locus_tag="RHV13_001463"
     CDS             complement(24637..25932)
                     /locus_tag="RHV13_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDV1241016.1"
                     /translation="MDADSSDSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASEYKPLSNSEWGKFALWLKHQRISPADLLVAQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWIITRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAMEALKHQWLPVQVKPNQDMQSANSRLVENGASWSTEQAENVTFR
                     LPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAYTPVDFYQLFVAELAILAKESIS
                     IERLASCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(25839..27935)
                     /locus_tag="RHV13_001464"
     CDS             complement(25839..27935)
                     /locus_tag="RHV13_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HDV1241017.1"
                     /translation="MEKHAAELLLQRMLGNTTATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGLTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLQGEVDCLLVSPERLANQDFIETVLYPIADRIGLLVVDEAHCISDWGHDF
                     RPDYRRILDILRQLPANTPVLGTTATANNRVVEDIRQQLGDIVIQRGTLARESLALDA
                     LVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDAELVAEWLRKNGISAFAYYSGVTCE
                     GAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFVIHYQMPGSIVGYYQQV
                     GRAGRAIDSAVGILLCGGEDRAIHKFFRESAFPAEAQIHEILNVLSVNDGLTLRGIEQ
                     RTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDHERINHLMNQRKSELED
                     VESYITTKECKMQFLRRALDEPGAERCGKCSSCLQRPLLSPDIDSGLLHAANLFIKHA
                     DLPLNLNKQVAAGAFTQYGFKGNLPAGLQGSTGRILSRWGDSGWGKQVAQEKKTGRFS
                     DELVEACAEMVRQRWNPHPEPTWVCCVPSLRHLDLVPDFARRLAVKLGLPFIDAIEKV
                     VDNPPQKMQQNRFHQCQNLDGAFVITPPLMPGPALLVDDIVDSAWTLTVLTALLRQAG
                     CPTVYPLALASTSVKN"
     gene            complement(28075..29415)
                     /gene="gntU"
                     /locus_tag="RHV13_001465"
     CDS             complement(28075..29415)
                     /gene="gntU"
                     /locus_tag="RHV13_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HDV1241018.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(29419..29946)
                     /gene="gntK"
                     /locus_tag="RHV13_001466"
     CDS             complement(29419..29946)
                     /gene="gntK"
                     /locus_tag="RHV13_001466"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDV1241019.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(30085..31080)
                     /gene="gntR"
                     /locus_tag="RHV13_001467"
     CDS             complement(30085..31080)
                     /gene="gntR"
                     /locus_tag="RHV13_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HDV1241020.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(31304..31999)
                     /gene="yhhW"
                     /locus_tag="RHV13_001468"
     CDS             complement(31304..31999)
                     /gene="yhhW"
                     /locus_tag="RHV13_001468"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HDV1241021.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(32122..33159)
                     /locus_tag="RHV13_001469"
     CDS             complement(32122..33159)
                     /locus_tag="RHV13_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDV1241022.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLNEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            33493..33981
                     /gene="yhhY"
                     /locus_tag="RHV13_001470"
     CDS             33493..33981
                     /gene="yhhY"
                     /locus_tag="RHV13_001470"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDV1241023.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYYNTLQVPHPSDYMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALVRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYFMAR
                     VK"
BASE COUNT         8394 a         9267 c         8433 g         7931 t
ORIGIN      
        1 tttcatcagg aatgaagaag aaacccttgc ttaaatgaat ctgatgaaca taagggaaac
       61 cagtattcac gctggatcag cgtcgtttta ggtgagttgt taataaagat ttggaattgt
      121 gacacagtgc aaattcagac acataaaaaa acgtcatcgc ttgcattaga aaggtttctg
      181 gccgacttta taaccattaa ttacgaagcg caaaaaaaat aatatttcct cattttccac
      241 agtgaagtga ttaactatgc tgattccgtc aaaactaagt cgtccggttc gactcgacca
      301 taccgtggtt cgtgagcgcc tgctggctaa actttccggc gcgaacaact tccggctggc
      361 gctgatcacg agtcctgcgg gctacggaaa gaccaccctc atttcccagt gggcggcagg
      421 caaaaacgat atcggctggt actcgctgga tgaaggtgat aaccagcaag agcgtttcgc
      481 cagctatctc attgccgccg tgcagcaggc aaccaacggt cactgtgcga tatgtgagac
      541 gatggcgcaa aaacggcaat atgccagcct gacgtcactc ttcgcccagc ttttcattga
      601 gctggcggaa tggcatagcc cactttatct ggtcatcgat gactatcatc tgatcactaa
      661 tccagtgatc cacgagtcaa tgcgcttctt tattcgccat caaccagaaa atctcaccct
      721 ggtggtgttg tcacgcaacc ttccgcaact gggcattgcc aatctgcgtg ttcgtgatca
      781 actgctggaa attggcagtc agcaactggc atttacccat caggaagcga agcagttttt
      841 tgattgccgt ctgtcatcgc cgattgaagc cgcagaaagc agtcggattt gcgatgacgt
      901 ttccggttgg gcgacggcac tacagctaat cgccctctcc gcccggcaga atacccactc
      961 agcccataag tcggcacgcc gcctggcggg aatcaatgcc agccatcttt cggattatct
     1021 ggtcgatgag gttttggata acgtcgatct cgcaacgcgc cattttctgt tgaaaagcgc
     1081 cattttgcgc tcaatgaacg atgccctcat cacccgtgtg accggcgaag aaaacgggca
     1141 aatgcgcctc gaagagattg agcgtcaggg gctgttttta cagcggatgg atgataccgg
     1201 cgagtggttc tgctatcacc cgctgtttgg taacttcctg cgccagcgct gccagtggga
     1261 actggcggcg gagctgccgg aaatccaccg tgccgccgca gaaagctgga tggcccaggg
     1321 atttcccagc gaagcgattc atcatgcgct ggcggcaggc gatgcgctga tgctgcgcga
     1381 tattctgctt aatcacgcct ggagtctgtt caaccatagc gaactgtcgc tgctggaaga
     1441 gtcgcttaag gccctgccgt gggacagctt gctggaaaat ccgcagttgg tgttattgca
     1501 ggcgtggctg atgcaaagcc aacatcgcta cggcgaagtt aacaccctgc tagcccgtgc
     1561 tgaacatgaa atcaaggaca tcagagaaga caccatgcac gcagaattta acgctctgcg
     1621 cgcccaggtg gcgattaacg atggtaatcc ggatgaagcg gaacggctgg caaaactggc
     1681 actggaagag ctgccgccgg gctggttcta tagccgcatt gtggcaacct cggtgctggg
     1741 tgaagtgctg cactgcaaag gcgaattgac ccgctcactg gcgctaatgc agcaaaccga
     1801 acagatggca cgccagcacg atgtctggca ctacgctttg tggagtttaa tccagcaaag
     1861 tgaaattctg tttgcccaag ggttcctgca aaccgcgtgg gaaacgcagg aaaaagcatt
     1921 ccagctgatc aacgagcagc atctggaaca gctgccaatg catgagtttc tggtgcgcat
     1981 tcgtgcgcag ctgttatggg cctgggcgcg gctggatgaa gccgaagcgt cggcgcgtag
     2041 cgggattgaa gtcttgtcgt cttatcagcc acagcaacag cttcagtgcc tggcaatgtt
     2101 gattcaatgc tcgctggccc gtggtgattt agataacgcc cgtagccagc tgaaccgtct
     2161 ggaaaacctg ctggggaatg gcaaatatca cagcgactgg atctctaacg ccaacaaagt
     2221 ccgggtgatt tactggcaaa tgaccggcga taaagccgcc gctgccaact ggttgcgtca
     2281 tacggctaaa ccagagtttg cgaacaacca cttcctgcaa ggtcaatggc gcaacattgc
     2341 ccgtgcacaa atcttgctgg gcgagtttga accggcagaa attgttctcg aagaactcaa
     2401 tgaaaatgcc cggagtctgc ggttgatgag cgatctcaac cgtaacctgt tgctgcttaa
     2461 tcaactgtac tggcaggccg gacgtaaaag tgacgcccag cgcgtgttgc tggacgcatt
     2521 aaaactggcg aatcgcaccg gatttatcag ccattttgtc atcgaaggcg aagcgatggc
     2581 gcaacaactg cgtcagctga ttcagcttaa tacgctgccg gaactggaac agcatcgcgc
     2641 gcagcgtatt ctgcgagaaa tcaatcaaca tcatcggcat aaattcgccc atttcgatga
     2701 gaatttcgtt gaacgtctgc taaatcatcc tgaagtacct gaactgatcc gcaccagccc
     2761 gctgacgcaa cgtgaatggc aggtactggg gctgatctac tctggttaca gcaatgagca
     2821 aattgccgga gaactggaag tcgcggcaac caccatcaaa acgcatatcc gcaatctgta
     2881 tcagaaactc ggcgtggccc atcgccagga tgcggtacaa cacgcccagc aattgctgaa
     2941 gatgatgggg tacggcgtgt aagtttagcc ggataacgcg ccagatccgg cttacatctc
     3001 tgcatcattc aatgctcacc cgcgttacgc catctgtttc tatcaaacta aaccgcaccg
     3061 gcaagaaacg ctccaccacc gcgatattgg tcagcagatg gcatgaggga tgggcgaccg
     3121 taaattcccc cgcgcccgcc agcgccatcg gtagtaccag ttggtcagca agatattccc
     3181 ccaccgccgc cgtgcttgcc aggtagcgtt tcacctcttt caccaactgt gccgcaacca
     3241 cctcggcact gacgcgcttt tcaccgacga caaaaaagcg ttcggtgata ttttcacttt
     3301 cgacttcaag cgagacggta ttacccggcc cctggtcgcg cggcaggtta tgaatattct
     3361 gttcatgcag ggaaaaactc cccgccagcg tagcgatttc acgctcagca acatggcgcg
     3421 gcacaccagc taatagaact tctccacgca tctgcacaat gttcccgcgc tcgccaagtt
     3481 gcaaggtgtt aaacgatgcc acgggcgaga cttccgttgc caccacaccg cctccggcag
     3541 ggtaaaaacc gtggcgtaac agcgtggttt gctgatgaat tcctattttc gccagcagcg
     3601 gctccagcac ccggcggata aaatccgcag gcggcgccga cgggttatcg gtgccgccgc
     3661 tcacttcaac acgcgaaggt ccatcggcaa accacagcgc gggcagcacc gtttgcagca
     3721 ccagcgtaca acttccggcg ctaccgatag caaagcggta atcgccgccg cgcacggtgc
     3781 cgggccggaa gagcagacgc tgcgacccca gctccgcgcc ttccacggtt gctccgcaaa
     3841 tttccgtcgc cgcctttacc gcggtcagat gctggcgcaa cagccccggt ttcgcccgcc
     3901 cggcacgaat gctggtgatg gtaaatggct ggccggttat catcgacagg ctcagcgccg
     3961 agcgcaggat ctgcccgcca ccttcgccct gtgcgccatc cagcgcaatc atccttttca
     4021 tcatttatcc ttttacgcac accacctgac gcagggtata gataacttcc accagatcgc
     4081 tttgtgccgc catcaccgca tcaatatctt tatacgccat cgggatttcg tcgatcactt
     4141 cggcatcttt acggcattcc acatgcgcgg tggcacgaat ttgatcttcc acgctgaaca
     4201 gttttttcgc tttagttcgg ctcattaccc gcccggcacc gtggctgcac gaacagaacg
     4261 actcttcatt tcccagccca cggacgataa agctttttgc tcccatcgaa ccgggaataa
     4321 ttccatattg accagcacgc gcagacaccg cgcctttacg cgtcacgtag atctcttcac
     4381 caaagtgctg ttctttttgc acatagttgt ggtgacagtt gatctcttcc atcgccagcg
     4441 tttgtggctg tctgaccgtt ttctgcgtaa tgctctgcaa tgccgttacc acgttttcca
     4501 tcatcgcatc gcggttaagg ctggcaaaaa gctgcgccca ggccacggct ttcaggtaat
     4561 catcaaagta ttccgtacct tccataaagt acgccagatc acgcgacggc aacgtctcaa
     4621 gcgtttcctg catctctttt tgtgccagat cgataaagta agtcccgatg gcgttaccaa
     4681 ttccgcgtga accggagtgc agcataatcc acacctggtc cgactcatca aggcagattt
     4741 caataaagtg gttaccggtt cccagcgttc ccaggtgttt atagttattg gtattcagga
     4801 aacggggata tttttgcgtt aaccactgat aaccggcttc aagctcagcc catttagcat
     4861 cgacgttaac aggtggattt tcccaggcac ctttatcacg tttacaacgg ccagtggtac
     4921 gcccgtgcgg cacggccgtt tcaatcgcct gacgcagctc tgccaggttt tcaggcaggt
     4981 cttccgccgt taacgcggta cgcagcgcgt tcattccaca gccaatatcc acgcccaccg
     5041 ccgccggaat aatcgcccct ttggtcggga tcacgctacc aatggtggaa cctttaccca
     5101 ggtgtacatc aggcattacc gcaatatgtt tgaaaataaa cggcatcttc gccgtattaa
     5161 taagttgctg acgcgcatcg gcctctaccg gcacgccttt ggtccacatt tttaccgggg
     5221 cattttcagt ggtcagtaat tcgtaattca ttttgttttt ctcttttcgt tgtttgctgt
     5281 cctgataatt gcaaccgtcg tgacagaaaa ttaaataagc agcttaattt tttaattcat
     5341 tgttttaaaa aagattatat ctttacgtcc gtaaccggag atttcccgca aagccaattt
     5401 accgataatg aaatatcgtc ttttataagg atatctaaga tgcgtaaaac agtggctttt
     5461 ggctttgtcg gtaccgtact ggattatgcc gggcgcggca gtcagcgctg gtcaaaatgg
     5521 cgtccgacac tctgtttatg ccagcaagaa tcgttggtca tcgatcgact ggaattgttg
     5581 cacgacgccc gctcgcgctc gctatttgaa acgcttaaac gcgatatcgc cagcgtttcg
     5641 ccagaaacag aagtggtgag cgttgagatt gaactgcata acccgtggga tttcgaagag
     5701 gtctacgcct gcctgcatga tttcgcccgt ggttacgagt ttcagccaga aaaagaagac
     5761 tatttaattc acatcaccac cggtacccac gtcgcgcaga tttgctggtt tctgctggca
     5821 gaagcgcgtt acctgcccgc ccggctgata caatcttcac ctccgcgcaa aaaagaacag
     5881 ccgcgcggcc caggtgaagt aacgattatc gatctcgatt taagccgtta taacgccatc
     5941 gccagccgct ttgccgagga acgccagcaa acgcttgatt ttcttaagtc cggcattgcc
     6001 acgcgtaacc cccacttcaa ccgcatgatt gagcagatcg aaaaagtggc gatcaaatcc
     6061 cgcgcgccga ttctgcttaa cggtccaacc ggcgcgggca agtcatttct ggcgcgacgc
     6121 atcttcgagt taaaacaggc gcggcatcag tttagcggcg cgtttgtgga agtgaactgc
     6181 gccaccctgc gcggcgatac cgccatgtcg acgctgtttg gtcatgtaaa aggcgcgttt
     6241 accggggcgc gggaatctcg tgaaggttta ttacgcagcg ccaacggcgg aatgttgttt
     6301 cttgatgaga ttggcgaact gggcgcagac gaacaggcaa tgctgctgaa agccattgaa
     6361 gagaaaacct tttacccgtt tggcagcgat cgccaggtga gcagtgattt tcagcttatc
     6421 gccggaacgg tgcgcgattt gcgccagctg gttgccgaag gcaaatttcg cgaagatctg
     6481 tacgcgcgga tcaatctctg gaccttcacc ctgccgggtc tacgccagcg ccaggaagat
     6541 attgaaccga acctggatta tgaagtggag cgccacgcct cactcactgg cgacagcgtg
     6601 cgttttaaca ccgaagcgcg gcgcgcctgg ttggcttttg cgacctctcc ccaggcaaca
     6661 tggcgcggta actttcgcga actttctgcc agcgtcacgc ggatggccac ctttgccact
     6721 agcggacgca tcactctgga cgtggttgaa gatgagataa accgtctgcg ctataactgg
     6781 caggagagcc gcccctccgc gcttacggcg ttgctgggcg cagaggcaga aaacatcgat
     6841 ctcttcgacc gtatgcaact ggaacacgtt atcgctatct gccgccaggc aaagtcgctt
     6901 tccgcagccg gacgccagct ttttgacgtt tcgcgccagg gcaaagccag cgtcaatgac
     6961 gcggatcggc tacgcaaata cctggcgcgt tttggtctga cgtgggaagc cgtgcaggat
     7021 cagcacagct ccagttgaat atggtggtcc gtcagcacct gcatcacgct tactggcggc
     7081 ggggcgtcgg tgtagacggc atctaccatg ctgatgctgc ccatattgac catcgcgtta
     7141 cggccaaatt tcgagtgatc gacaaccagc ataacgtggc gcgagttctc aataatggcg
     7201 cgtttggtgc gaacttcgtg gtaatcgaac tccagcagcg agccgtcgct atcgatgccg
     7261 cttatcccca gaatgccgaa atcaaggcgg aactgggaga taaaatcgag cgtcgcttcg
     7321 ccaatgatcc cgccatcgcg gctgcgtaat tcgccaccgg cgagaataat gcggaaatct
     7381 tcttttacca tcaacgtgtt agcaacattg agattgttgg tgacaatacg caaattgctg
     7441 tgattaagca gcgcgtgcgc caccgcttcc ggcgtggtgc cgatatcgat aaacagtgtc
     7501 gagccattgg ggatttgctc cgccactttg cgggcaatcc tctctttttc ttcggtctgg
     7561 gtggctttac gatcgtgcca cggcgtgtta accgaactgg aaggcagcgc cgcaccgcca
     7621 tggtgacgca ggatcaggtt ttgctccgcc agctcattga ggtcgcggcg aatagtctgc
     7681 gggctgacgg agaagtgctc caccagctcc tcggtactga cataaccctg ctgtttaacc
     7741 agttcgataa taccgttgtg acgttgtgtt tgtttcattt ctaaatccct ggaattattt
     7801 tcgttttcgc gcattgagcg aatcaacaaa agccatcgct aaacccacgg ctaacccggc
     7861 gatgtgtgct ccgttcgcca tcgacatccc aaacaaatca aaccatccgg cgacaatcca
     7921 gatcagcgca aagataatta acccacgttg caggtaaatg ccactttgcg gatcgcgttc
     7981 gccacgtagc cagacgtagc ccatcagcgc atacaccacg ccagaaagcc cgccaaacca
     8041 cggcccgctg aatttttgct gcacatagcc gcttaacagg gcgctgataa gcgtaatgac
     8101 aattagctta ccgctaccga ggcgtttttc caccgcaccg ccgagatacc accaccagag
     8161 caggttaaag aggatatgca ttagtgagaa gtgcattaac gcgtgggtga agtaacgcca
     8221 gaactcaaat ttcagtgttg gatcgaatgg ccaggccagc cataacatga cttcctgatc
     8281 gccgagaatt tgcatggcga taaacaccac cacgcaggca atcatcatca cccaggtcac
     8341 cggacctgca cgttcacgca aggcggcaaa gaaaggataa cggcgataat gcaggccact
     8401 gccggtatga cccgcctgcc agctggccgc cagataacgc ggatctgccg tgttttcgag
     8461 aaaacgcgcc agctccgccc gcacgcgctc ggcctgggac tcatccgcca gccagacatc
     8521 gctttggtta tgttgttgaa tcgtgaggat aacaccctgc gttgccatgt aatcaacaaa
     8581 cgcctgcgcc acgcgggggt tagcaaaaga ggtaatcatc aacatcgttg ctgtcgctta
     8641 ttccacacaa aaggggacag tataaagcgt tacgcgccgt acgccacctc tgcgggaaac
     8701 tgacgctgcc aggcttcaaa gccgccgtca atgctataga ccacatcgta gccctgttgc
     8761 agcagatact gcgccgcgcc tttgctgcta ttgccgtgat aacacatcac catcaccggt
     8821 gtgtcaaagt cgttatcacg cataaacgcg cccagcgtgt cgttggttaa atggaaagcc
     8881 tgcaccgcat gtcccattgc gaaactctgt ggatcgcgaa tatcgaccag caccgcctct
     8941 ttttcctgca acttctggtg cgcgtcggca acgttaatac attcgaactg atccatgcgt
     9001 ctctctttct ttacaaacaa gtgggcaaat ttaccgcaca gtttacgtcg aagcggcaga
     9061 taaacgccat aatgttatac atatcactct aaaatgtttt ttcaatgtta cctaaagcgc
     9121 gattctttgc taatatgttc gataacgaac atttatgagc tttaacgaaa gtgaatgagg
     9181 gcagcatgga aaccaaagat ctgattgtga taggtggcgg catcaatggt gctggtatcg
     9241 cggcagacgc cgctggacgc ggtttatccg tgctgatgct ggaggcgcag gatctcgctt
     9301 gcgcgacctc ttccgccagt tcaaaactca ttcacggtgg cctgcgctac cttgagcact
     9361 atgaattccg cctggtcagc gaggcgctgg ctgaacgtga agtgctgctg aaaatggccc
     9421 cgcatatcgc cttcccgatg cgttttcgcc tgccgcatcg tccgcatctg cgcccggcgt
     9481 ggatgattcg cattggtctg tttatgtacg atcatctggg taaacgcacc agcctgccgg
     9541 gatcaactgg tttgcgtttt ggcgcaaatt cagtgttaaa accggaaatt aagcgcggat
     9601 tcgaatattc cgactgttgg gtagacgatg cccgtctggt gctcgccaac gcccagatgg
     9661 tagtgcgtaa aggcggcgaa gtgcttactc gtactcgtgc tacttctgct cgtcgcgaaa
     9721 acggcctgtg gattgtggaa gcggaagata tcgataccgg caaaaaatat agctggcaag
     9781 cgcgcggcct ggttaacgcc accggcccgt gggtgaaaca gttcttcgac gaggggatgc
     9841 atctgccttc gccttatggc attcgcctga tcaaaggcag ccatattgtg gtgccgcgcg
     9901 tgcataccca aaagcaggcc tacattctgc aaaacgaaga taaacgtatt gtgttcgtga
     9961 tcccgtggat ggacgagttt tccatcatcg gcaccaccga tgtggaatac aaaggcgatc
    10021 cgaaagcggt gaagattgaa gagagtgaaa tcaattacct gctgaatgtg tataacgcgc
    10081 actttaaaaa gcagttaagc cgtgacgata tcgtctggac ctactccggt gtgcgaccac
    10141 tatgtgatga tgagtccgac tcgccgcagg ctattacccg tgattacacc cttgatattc
    10201 atgatgaaaa tggcaaagca ccgctgctgt cggtattcgg cggtaagctg accacctacc
    10261 gaaaactggc ggaacatgcg ctggaaaaac taacgccgta ttatcagggt attggcccgg
    10321 catggacgaa agagagcgtg ctaccgggtg gcgccatcga gggcgaccgc gacgattatg
    10381 ccgctcgctt acgccgccgt tatccgtttc tgaccgaatc gctggcacgt cattacgcgc
    10441 gtacttatgg cagcaacagc gagctgctgc tcggcaatgc gggagcggta agcgatcttg
    10501 gggaagattt cggtcatgag ttctacgaaa cggagctgaa atatctggtc gaccacgagt
    10561 gggtccgccg cgccgacgat gccctgtggc gtcgcacaaa acaaggtatg tggctaagtg
    10621 cagaacaaca atctcgtgtg agtcagtggt tggtggagta tacgcagcag aagctgtcgc
    10681 tggcgtcgtg atttaatgta aggtggccag gtcagatttc aatctgacct gagactgatg
    10741 acaaaccaaa aaactgccag atgcgctacg cttatctggc ctacgttgtt tatacaatat
    10801 attgaatttg catgattttg taggcctgag aaggcgttca cgccgcatca ggcatgaaca
    10861 aagtgcactt tgtcaacaat ctgatctact ttttgagtca gagaattacc gtaaagccca
    10921 cgccatcata cccaacgtaa caaaactcag gcacgttccc cactcactcc acttccgtat
    10981 ctggcttcta tttttgctac gccactggca ataatatccc aacgcaaaac caagctgtgc
    11041 gcttattgga ccgataaaat ccgtccacgg ctgaggccaa aatggcgaaa aattcagggg
    11101 tagccagaca acaagcagaa agaaattgcg gatactttcc agcaacgcaa ttttcgctgg
    11161 cgcgacatag aaaatgatat tccacaggag caaccagaaa taaactggta caaataccag
    11221 ccaataatgc agtttgcttt tcaattcaga ttgagttatg cgagcaaatt gccaaccaac
    11281 gcaacataag gcgcagaaca aaagcgtata ttcagccaat aacccaagtt gtaattgcag
    11341 aagtgaaata cgcctgatca tgagcgagat tgagcacagc atttccccca gaaaactcag
    11401 cgtgataata caagcagcca gcgcaaccac cgtcggccac accaacaacg gcacataccc
    11461 cacatcctct accaactgtt gcccctgcgg acttaaaaac cagtcaacaa atttctgcgt
    11521 ttccggcgtg gcattttcac gcgtcaccat aaatgcatcg acgatgtacg catatttgcc
    11581 gttgcgaatg ttttccgccg tcggtgtaat atcgttaatc gctagcaatt ttatattttt
    11641 atcagcattc atttgcgtcg cgtaatagcg gaaggtatag cctattgcgt tgtttgtatt
    11701 acggtattcg gcaacaactt taatcattcc ctccatcacg cttgccactt cggtttcctg
    11761 cggcgagatc atgcggacat ttttcatgac ctgtgattgc atcaccgtct ggctgccaga
    11821 gtcttccggg cgttgccagg tctggatctc ctgatcgtta ccgccaacag tacgccagtt
    11881 ggtaattgca ccactgaaaa tgtcacgcac ctgttgttca gtcagggaat taaccggatt
    11941 atccgcattg acgatgaaaa cgaatgcttc acgggcaaat ggcgtgtata gcaaagtgac
    12001 gcccgattcc tcagcgcgtt ttttctgcct accggagggt tgcgccacaa aaataatatc
    12061 ggcatcacct ttaacaatcc tgttatatgc atcgggggta cgagagttct ccagatattc
    12121 cctggtgtgg aaatcttccg gtataacgct caacgcataa aaggcagagg cataaattgg
    12181 gtacgccgcc gtagcaccat cgagtcgcgg ccagttttgc gtaaactgaa tttgcggttt
    12241 accacgtaat ggtgtcagtt gattattgag tttatcgggt cgccaggccc aggtatcaat
    12301 attttcgcgc acaccgacgc cgggattttg ctgcttatat aaatgggcct gatacccggc
    12361 gacaatacca agtaagagaa gtagaaacag gagtaagtta cggcaacgta gtccggacgt
    12421 gccggataaa ggatatctgg tcggccaagc gtatcccagc gaaaaactga tatgcgcccc
    12481 aacgggaata atgaccatcc accagtactc accacccact aatgagacta aaaaaacgcc
    12541 caaccaggga agcataaggt ttgccaacca accagctccc atctggcgaa attgataatc
    12601 tgccagatgc agcgcgatta tccagccaac cagggttaat agcgcgggta agagaaaaaa
    12661 gcattgccgc cagacggcta tttctctgtt ttctggcgcg ctatcaccgt cccacacgcg
    12721 accagagaaa aatattatta tcgcccccag cgtcagcata aagacgttga gtagaagata
    12781 aattgttggc gtgataagga tattacaaac ttcaataata ccggcaagtc cgacacccag
    12841 catagcgaca accgccgcca aaaattgcgt cagtatgggg atgccaaaaa aagtcatcac
    12901 cagcgaggac aaaatccatt ttctgttttg cattattctt tccattcttt ctgaatggcg
    12961 aaattatact ccccgagtcc cctcgccccc tctggacact tttccgaaat aaaggcgaaa
    13021 aaaaacggga ccttgtggcc ccgttctgtt ttttagcgaa cttacaatct cactggatca
    13081 atatgccaga tatgatcagc gtactctttg atagtacggt cagaagagaa gtagcccata
    13141 ttggcaatgt tcagcatcgc ttttgcggtc cactcttcct gaagctcgta gagttcatcg
    13201 actttatcct gacaatcgac atagctgcga taatccgcca gtacctggta gtgatcgccg
    13261 aagttgatca gcgaatcaac cagatcgcga tagcgacccg gatcttccgg actgaataca
    13321 ccgctgccga tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc
    13381 ggtttgtagc cttgacgacg cagttcttcc acttcttccg ctgtgttacc aaagataaag
    13441 atattgtcag caccgacatg atccagcatc tcgacattcg caccgtccaa cgtaccgata
    13501 gtcagcgcac cgttaagcgc aaacttcatg ttactggtgc cggaagcttc cgtccctgcc
    13561 agcgaaatct gttcagacag atcggccgcc ggaatgatca actgcgccag gctaacgctg
    13621 tagttcggga tgaacacgac tttcagcttg tcgccaatct gcggatcgtt gttgatcact
    13681 ttcgctacgt cattgatcaa atgaataatg tgcttcgcca tgtaataggc cgaagccgcc
    13741 ttaccgccaa aaatattcac gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg
    13801 ttatagcggg taatcacatg caacacattc atcaattgac gtttgtattc gtgaatacgt
    13861 ttgatttgca catcgaacaa cgcctttgga ttcaccacca cattcagctg ctgggcgata
    13921 tactccgcca gacgcttttt attctccagc ttcgcctgat gcacagcgtg attaaccatt
    13981 gggaaatcac agtgttgttg cagctcatta agcaggctaa ggtcggtgcg ccagttacgg
    14041 cccaggtgtt cgtccagcac ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc
    14101 ggcgtcacac cgttggtgac gttggtgaaa cgacccggga agattttcgc aaagtcggca
    14161 aacaacgatt gcaccatcag attagagtgc agttccgata caccgttaac tttgtggctc
    14221 acaacaaccg ccagccaggc catacgcaca cgacgaccgt tggattcatc aatgatcgac
    14281 gcccgtccca gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag
    14341 tcgttgattt caaagatgat ctgcaggtga cgcggcagaa ttttacccag catatcaacc
    14401 ggccaggttt ccagcgcctc gctcatcagc gtgtggttag tgtaggagaa gacctgacaa
    14461 cacacctcaa acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag acgcatcatc
    14521 tcaggaatcg acagtaccgg atgggtatca ttgagatgaa tcgcgatttt atccgccagg
    14581 ttatcgtagg ttttatgcaa ctgataatgg cggcttaaaa tgtcctgaat ggtcgaggaa
    14641 accaggaagt attcctgacg caggcgcagc tcacgcccgg agtaggtgga gtcatccgga
    14701 tacagtacgc gagatacgtt ctcggagtgg tttttatctt ccactgccgc gaagtagtca
    14761 ccctggttga atttaccgag gttaatttcg ctactggctt gcgcactcca caaacgcagc
    14821 gtgttggtcg cgtcggtgtc gtaaccaggg attatctgat cgtaagcgac tcccagaatc
    14881 tcttcggttt caatccagcg cgttttttta ccttcctgct gaatgcgacc accaaaacgg
    14941 actttatagc gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc cagccagtag
    15001 tctggcgact ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag
    15061 cggatgccgt aaccgcgccc cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc
    15121 gccagacgtc ccaggccacc gttaccgagg cctgggtcat tttcttcatc aatcagctct
    15181 tcgagattta accccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac
    15241 aacatggcgt tggagagcgt acggccaatc aaaaactcca tcgacaggta gtaaacctga
    15301 cgagtttctt gcgacaactg ggcacggttt gaacgtaacc agcgctccac gagacgatcg
    15361 cgcacagcaa ataacgttgc gttcagccat tcatgtttat tggcgacgac cgggtccttt
    15421 ccaatcgtaa acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg
    15481 ggcgatgaat atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga
    15541 tagtaaagct cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc
    15601 tgacgttgca caaaccgcca cagtgaagga cgggaccaca gtacaaaagc acgtcgaata
    15661 gcccgtaata gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct
    15721 gcaaggttct caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact
    15781 aacggcagcg taccgtactt caatccataa agttgcgtta agccgcacgg ttcaaaacgg
    15841 ctgggcacca gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag
    15901 ccaatctgaa cgcccacctg gccggggtat tccgctgccg ccgcaaggaa accttcctgc
    15961 agcaccggat cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aaggcccggt
    16021 aaggcttcca gcaccaggtc gagacctttc tggctggtca gacggctcac cactgcaaaa
    16081 agcggcactt tatcgtcaac cttaagcccc attgcgattt gtaactggcg cttattttcc
    16141 gctttatctt ccaacgtatc gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga
    16201 ctccagattt tctcgtccac gccgttcagt acgccggaaa gacgcccttc acggtgacgc
    16261 tgttgcaaca gaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag
    16321 gttggactga ccgccgtaat gtgatcggca tagtacagac cggccttcag gaaagagatt
    16381 tgtccgttga attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc
    16441 atgtgatgtg cataaaacat gccttgatag gccaggttgt gcacagtaaa caccgacttc
    16501 gccggacgcc cgcgcgccgc cagatacgca ggcgcaaggc ctgcatgcca gtcgtgcgca
    16561 tgcaccacat caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac
    16621 cccagcagcg caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac
    16681 gggcttcccg gacgatcata gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg
    16741 taatgaccga acaacagcgt gatatgtccg gcgaaggtat cacgacggga tactacctgc
    16801 gcatcggtca cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg
    16861 ccgtctgcga tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta
    16921 agcagcggga acatctctga acatacatgt aaaacctgca ttatcgctcc tgtttatgcc
    16981 ctaacttccg tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac
    17041 gacgtgcatc ttcctctgcg ttttcaccaa tcaccatgcc ttccggaata acacaagcac
    17101 gatcgatgac gcagcggcgc agacggcacg agcgacctac ccatacttcc ggtaacaata
    17161 cggcggaatc aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca
    17221 ccaccaccga accggagatc acacaaccgc cggaaaccag tgagttaagg gtcatcccgt
    17281 ggctaccgga gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa
    17341 ttggccaatt gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgaggttcg
    17401 ctttccagta agcttccagc gtacccacat cgcgccagta cggctcggca tccgggtcgg
    17461 attgtacgca agagagcggg aacgggtgcg cataggccag accggcttcg gtgatcttgg
    17521 gaatcaaatc tttgccaaag tcgtggctgg agttctcatc gcgatcgtct tcttccagca
    17581 gttcatacag atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg
    17641 gatcgttcgg cattgacggc gggttagcag gtttttcaac gaattcgata attttatcgt
    17701 tctcatcaac cgccataacg ccaaatgcgg aggcttcttc aatcggtact ggcatacaag
    17761 caacggtgca acgtgcgcct ttttcgacgt gatcgataag catacgcgag tagtcttgct
    17821 tgtagatatg gtcgcccgcc aggatcacca cgtattccgc tttataacgg cggataatat
    17881 cgaggttttg ggtgaccgca tctgcggtgc cgcgatacca gttttcccct ttcattctct
    17941 gctgtgctgg cagcagatcg acaaactcgt tcatttcttc attgaagaat gaccagccgc
    18001 gctgaatgtg ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga
    18061 tcccggagtt gatgcagtta gacagcgcaa agtcgataat gcggaactta ccgccgaagt
    18121 gtacggccgg ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg
    18181 ccagtatcag ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct
    18241 tctctaaact aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg
    18301 tgcaggtccc tgccagacag ccgtaatcac tgggttatcc tctccagcga atgggggaat
    18361 ggcgtgccac tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc
    18421 gatcaaaaag cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc
    18481 cgtgcttaaa ggttgagcat atcgatttag ccaacggaca ttgccgtcgc cttcttccca
    18541 ccagcgattc tccaccaaag cgggaatacg cttgcgcaga tggattaacg cggcggtaaa
    18601 tgcggttaaa ccactgcttg cctgcgacca gtccaaccag gttaattggt tatcctgaca
    18661 gtaggcattg ttattgccat gctggctgtg accatgttcg tcaccggcca gtaacatcgg
    18721 cgtgccctgg gagagcaaca acgttgttaa cagggcatga atgctgtcgc gccgccgttc
    18781 aaccagatca agagtaccgc ctaacccttc tttaccatga ttgttactgt aattgttgtt
    18841 ggtcccgtcg cgattttctt ctccgtttgc ttcattgtgt ttatggttga agcaaacgca
    18901 gtcgcgaagc gtaaaaccgt catgtgcggt gacgagatta atcgcggcac tcggcagacg
    18961 accattacgt ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg cccccagagg
    19021 caaatcataa tgcagccaga aacgacgggc agcatcgcgg aaatgatcgt tccactcggc
    19081 aaacagcggc gggaaatttc ccacctgata accaccagga gcgatatccc acggttcagc
    19141 aattaacttc acctgcgaga gcaccgggca gttctggata gcggtaaaca acggcgcatc
    19201 ctgacggaac tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac
    19261 gtgacaggtt tctacccaat aacgcaggca ggcgcaggca taatccacca ccgccggatg
    19321 actcaaattg agcgtgttgc cacaaccggt ccagttgtga taatcgccgt cttctcttat
    19381 ccaataatag ctacggttgt cgatcccacg cagcgagaat aacgggccgt cgaggtccag
    19441 ttccgcacta tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc
    19501 tttgattgca tcgcgaaact catgcagcgc cgtttctggc gagcaggcat acgccggatg
    19561 cagcgcaaac atcgccaccg ggttgtaacc ccagtagtta cttagcccca tgcgttgcag
    19621 acgtggttca ctggcaaact gcgccaccgg cagcagttcc agcgcggtaa tgcccaactg
    19681 tttcaaatag ttgatcatca ccggatgccc gagggcttta taagtgccac ggatctcgac
    19741 cgggatctcc gggtgcaggt acgttaatcc tttaacatgg gcttcataaa tgatggtgct
    19801 gccccacggc gtgcgcggcg gggcatcatc ttcccagtca tagtgatcaa ccaccactac
    19861 gcatttcggc gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag
    19921 cagcgggtta tctttgaatt ccccttcaat ttgccgcgcg caaggatcaa tcaacaactt
    19981 cgccgggtta aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc gataaccata
    20041 acgcaaaccc gggcgcgcat ccggcagata accgtgccaa atgtcgccac tgtgccctgg
    20101 caagtcatag cgatgttcct ggccattggc gtcaaagaca cacagctcta cccgctcggc
    20161 atgagcggag aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc
    20221 gggtttgcca atggcgagtc gtgtcattct gcctcccgaa ccagccagat agtggccagc
    20281 ggtggtagtg tcaggcttag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt
    20341 accgtgccgc cattgcctgc attactgccg tgatagtgca tggaatcggt attgaggatt
    20401 tcacgccatt tgcctggctg attaatgccg aagcgataat catgacgcgg caccggcgta
    20461 aagttactgg caacgataat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc
    20521 gagcgttctt tgtcatccac caccagccat tcaaagccgt acgggtcaaa atccagttca
    20581 tgcatcgctt tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg
    20641 tggtgccagt tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc atggttccac
    20701 tcgcggccct gggcaaattc gttccccatg aacagcagtt tcttgcccgg aaatgcccac
    20761 atccagccat agtaagcgcg caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg
    20821 tcgagaatcg attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt
    20881 tcagtgtagt tgtagagcat cccgaaggtc agtttatcgt gatgatactg acgataaatc
    20941 gggtcgagct tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag
    21001 aagcccagac cgcccatatc ctgcggacga gaaacgccag ggaaatcggt agactcctca
    21061 gccattgtca ccgcaccgga aacctgctca ccaagaatac ggttggtatt acgcaagaat
    21121 tcaatcgctt caagattctc gcgcccgcca aattcgttcg ggatccactc cccctcttta
    21181 cggctgtagt cgcgataaat cattgacgcc accgcatcga cgcgcaacgc atcaatacca
    21241 aaacgttcaa tccagtaaag cgcgttaccg acaaggaagt tactgacttc acggcgacca
    21301 tagttgtaga tcagcgtgtt ccagtcctga tgatagcctt cacgcggatc gctgtgttca
    21361 tacaagttcg tgccatcaaa ttcggcaagc gcaaagtcat cggtcgggaa gtggcctggc
    21421 acccagtcga gaatcacgtt cagaccagct gcgtgtgcgg catcaatgaa ataacggaag
    21481 tcgtcgcgag taccaaaacg gcgggttggt gcatacaggc cggttggctg ataaccccaa
    21541 ctgccatcga agggatgctc gttaatgggc agtagttcga ggtgggtaaa gcccatccat
    21601 ttagcataag gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttgtcg
    21661 gtgtggcgac gccaggaacc caggtgaact tcataaatag agattggcgc atcaaactga
    21721 ttcgcttttt tgcgctcttc agtctgtaca accttttccg gcagcccgca aataagagac
    21781 gcggtttccg ggcgcatttg cgcttcgaag gcataagggt cggacttcag acgcaagttg
    21841 ccattggcat caatcatctc gtatttatag agctgaccgt tatgcgcccc agggataaac
    21901 agttcccaga tgccgctctc tttacgcagg cgcatcgggt gacggcgacc gtcccagtag
    21961 ttgaattgcc caaccaccga gacccgacgg gcgtttggag cccagacaga gaaacgcgta
    22021 cctgtgacgc catccatagt atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga
    22081 gtaccttcag ataatagcca ggcatccatt tcctggatta gcggaccaaa acggtaagga
    22141 tcatcaatca ggttttgctg accatgccag acaacagcca actgatagcg gaaaaaattc
    22201 ttacgtcgcg gaatgacgcc gctaaagaat ccccgtgagt cgagacactc cagttttgcg
    22261 agtttgcgcc cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca
    22321 cggacttcca gtcccgcggt ggttttatgc attcccagta cggaaaaagg atccgcaaaa
    22381 tggcctgcaa ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc
    22441 ctgttttatt gtgtcacccc atccaattgg atttttgact tctggttgtg acattttttt
    22501 gacctgaacg gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc
    22561 tgtgaatact catgtattca gccaccctta aagaatagcc aatgctctat ttaactcccg
    22621 gtaaatcatg aaacatctgc gcttactcct gtattacgca ctaacagggg cggcatcgcg
    22681 ccccagattt aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg
    22741 cggcccccca cagcagctgg tcgcccactg taaaggctga caggaactct ggtcccatat
    22801 tcagcttacg caggcggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta
    22861 gctcacgcat agtgatttcc cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag
    22921 ccagcagttc ttccacggtc ggaatagaca catctttttt caatttaata gtgaatgcct
    22981 ggctgtggca gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag
    23041 atgtgttgag gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt
    23101 tatcgagctg tttgtcgatc cacggaatca ggctacccgc caacggcacg ccaaagttat
    23161 ccaccggcag ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag
    23221 cagaggacgg ggtcgcgagt tcatctgcca catggccata cagatggccc atctgggtta
    23281 ataactcacg catatgtcgc gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca
    23341 cccaatcaac aagatcattg gcgaataaac cacccaacga catcaacatc aggcttacgg
    23401 tacagttacc gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct
    23461 gattgacggg gtcaagaatg atgatggcgt catctttcat gcgcagagac gatgctgcgt
    23521 caatccagta accttgccat ccgctttcac gaagctttgg atagatttcg ttggtataat
    23581 cgccgccctg acaggtcaca atgatatcga gggcctttag cgcctccaga tcaaaggcat
    23641 cctgaagtgt gccagtggtt ccgccaaaag acggcgcagc ctgaccaagc tgagaagtag
    23701 aaaagaagac agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa
    23761 cggagccgac cataccgcgc cagccgataa aaccaacatt tttcataagc gttttttcct
    23821 gcaaagatgt gtgctgtata aatgtgccgg tctcctcttg gcacatcttt caccatacaa
    23881 aaagcagcca aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac
    23941 ttatcctgct ttgttagcac gcagaaagtc cgcggcaatt atcagggaat ttgagttatg
    24001 aatgaaatca tttctgcagc agttttattg atcctgatta tggatccgct cggaaaccta
    24061 cctattttca tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg
    24121 cgagagttgc ttattgctct cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt
    24181 ctggcatttc tcagcctgcg tgcggaaacc gtctccattt ctggcggcat cattctgttt
    24241 ctgatcgcca ttaagatgat tttccccagc gcttcaggaa atagcagcgg gcttccggca
    24301 ggtgaagagc catttatcgt gccgctggca attccgctgg tcgccgggcc aactattctc
    24361 gccacgctga tgttgttgtc tcatcagtac ccgaatcaga tgggacatct ggtgattgct
    24421 ctgctgctgg cctggggcgg cacctttgtc atcctgctac agtcttcgct atttttacgt
    24481 ctactgggcg agaaaggggt gaacgcactt gaacgcctga tgggattgat tctggtgatg
    24541 atggcaaccc agatgttcct cgacggcatt cgaatgtgga tgaaggggta aggttcaaac
    24601 aggtgctgca cacaaccaga cgcggcacct gttttattac ctgaactgat aatgaccttc
    24661 gcccaatcgg ataacccttc cctcttcttc tgcgcggttc agccacacac taatttgttc
    24721 gatggttaaa ccagtacaag acgccagcct ttcaatactt atcgattcct ttgcaaggat
    24781 cgccagttcc gccacaaaga gttgataaaa atcgacaggc gtatacgcgg gcattactgc
    24841 gtaatttgcg tcatcttcat gcagcgaaaa cagttctggt tgagcgttac ggagtgctct
    24901 gtcatacatc agcccaggaa cgtctggcag tctgaacgtc acattctcag cctgttcagt
    24961 actccatgac gccccatttt ctactaatcg tgaattggct gattgcatat cctgatttgg
    25021 tttaacctgc acaggcagcc actgatgttt taatgcctcc atcgccccgg ttatcgttcc
    25081 accggtcatt cccgcacgta caaccattgc gctttcagca aggcaataaa tatatttatt
    25141 tcgcgccatc gcattgccga cggtgaaacg tacctctggg taaaacggcg aaatcaacac
    25201 caggttgcct gctataagcc cttcacgcca tttcactaac gtgctcgttt ttaacaagct
    25261 atcagctaat acgccaacgg cagttccccc ggcctccagt gctgacgcca ttgcacattc
    25321 atcaatacct cgcgcaccac cagagataac gcaaatcccc tgttgggcca gtttcgctgc
    25381 cagttgttgg gtatagcgca aatcgtcagt cggagcatct cgcgagccaa caatcgccat
    25441 accttccgct tgcagtaatg ctttattccc gcagccaaat aaaacgggag gtgcatccgt
    25501 tcgcaatcgg tttttcaagc gaacgggata atcagcatct ccgcgggtta tgatccataa
    25561 accggcgcgt tgccacttat ctaccgccaa cgccagacta tgaccgcgcg ccagcaagcc
    25621 aagaatacgc tcctgagaaa tacgcggatc gctccagcct gtaagtttct cttgcggttg
    25681 cgccaccaga agatcggcgg gactgatacg ttggtgcttc agccataatg caaacttccc
    25741 ccattcacta ttactaagag gtttatactc actcgccgcc gcgcgagaaa aatcgctggt
    25801 tagcaggaga gttgcttgtg cattggctga aagattcatc aattttttac cgaggtagaa
    25861 gcaagggcaa gaggataaac cgtcgggcaa cctgcctggc gtaacagtgc tgtcagaact
    25921 gtcagcgtcc atgcagaatc cacgatatcg tcaaccagca acgccggacc tggcatcaaa
    25981 ggaggggtaa tcacaaacgc cccgtcgaga ttttgacact ggtgaaaacg gttttgctgc
    26041 attttctgcg gtggattgtc cacgactttt tcaatggcat caataaaagg taagccaagt
    26101 ttcaccgcca gtcgccgggc aaaatcagga accaggtcga ggtgccttaa tgaaggaacg
    26161 cagcataccc aggttggttc aggatgcgga ttccagcgct ggcgaaccat ttccgcacat
    26221 gcctccacca gctcatcact gaaacgcccc gtttttttct cttgtgctac ctgctttccc
    26281 cacccggaat ctccccaacg agaaaggatt cgccccgtag atccttgtaa acccgcaggc
    26341 aagttccctt taaatccgta ttgcgtaaaa gccccagctg ccacctgttt attgagattt
    26401 aatggcaggt cagcatgttt aataaataaa tttgccgcat ggagtaaacc gctatcaatg
    26461 tcgggcgaca acaacggacg ctgaagacaa ctgctacatt taccacaacg ttcggcacct
    26521 ggctcatcga gtgcgcggcg cagaaattgc attttgcact ccttggtcgt gatatagctt
    26581 tcaacgtctt ccagctcact ttttctctga ttcatcaaat ggttaattcg ttcatggtca
    26641 ggagaaaaac tgacaattgt tctgcgccat aatttctcgg tatacaccac aggcgatgga
    26701 ttttcagcta ccaataattt cagtgctttt tctatttgcc cataacggag attcgtccgt
    26761 tgttcaatgc ctcgtaaggt aagaccgtca ttcacgctaa gtacgttgag tatttcatga
    26821 atttgcgcct ccgcaggaaa ggcgctttca cggaagaatt tatgaatagc gcggtcttca
    26881 ccaccacaaa gcaatatgcc aactgcggaa tctatagcac gcccggcacg ccccacctgt
    26941 tgatagtaac cgacaataga ccccggcatc tggtaatgaa taacaaaccc caaatccggt
    27001 ttgtcaaagc ccattcccag cgccgttgtc gcaaccagca ctttaatttt atttgccagc
    27061 aacgcctgct ccagatattc cctggcagta ttagaatctt ccgcgccttc acaagtcaca
    27121 ccgctgtagt aagcaaatgc actgatacca tttttcctta accactcggc gacaagttcg
    27181 gcatcgcgag tagtcagggt ataaacaatt cccgatttgg aaaactgagg gataaccgtt
    27241 gctaaccatg ccagacggga tgactgttca cctaagacta aggcatcaag cgccaaactt
    27301 tcgcgagcca gcgttccacg ctgaatcaca atatcaccca attgctgacg gatatcctca
    27361 acgacacggt tattcgctgt cgcggttgta cccagaacag gggtattcgc aggtagttgg
    27421 cgcaaaatat ctaatatacg tcggtaatcc gggcgaaaat cgtggcccca atcagagatg
    27481 cagtgcgcct cgtcgaccac aagcaagcca atacgatcgg ctataggata taaaaccgtt
    27541 tcgataaagt cctggtttgc caaacgttcc ggggaaacca gcaagcaatc gacctcacct
    27601 tgcagcaact tatcgctaat gcgctgccat tcttctctgt ttgtagaatt caacgtttca
    27661 gccgtaatac ctaaccgttc agcggcggca acctggttac gcatcagggc aagcaaagga
    27721 gagatgataa ttgtcaggcc agcgccgcga tcgcggaaga ttttactggc gatgaagtac
    27781 acggcacttt tcccccagcc agtgcgttgg acaaccagca gttttcgccg ttggttcacc
    27841 accgcgtcta tggcttccca ttgcccttcc ctgaaagtag cggtggtatt tcctaacatc
    27901 cgctgtaaaa gcaattcagc tgcgtgcttt tccatcttta tctcctgcga tctttaggca
    27961 gtgattagaa cggattattt gcaggtaaat ttgcgagaga gatcaaaaag atgaagagag
    28021 gagaaaagag aaatgaatta ctgcgagcaa gctcgcagta atttgtcgca gtaattaact
    28081 caacagctgg aacgcaatca tcccaacgat ggcaccgaca gtgccgagga tggtttccat
    28141 catggtccag gttttcagcg tttcggcttc ggtcgcgccg gtaaatttac cgaacaacca
    28201 gaaaccggcg tcgttaacgt ggctgacaac aatcgaacca ccggcgatac aaatcgacag
    28261 cgccgccatt tgcgcaccgg agtagttcag ttgttcaatg actggcatca ccagtcccac
    28321 cgccgttaaa caggcaacgg tggcagaacc ctgaatgatg cgcactgcag cggccagcac
    28381 gaagcaggtg atagcaatcg gcaggcccat gccggttaac gcttcgccca gtgccggacc
    28441 tacgccagag tcaaccagca cctgtttgaa cacgccgccc gcaccaatca ccagcagaat
    28501 gatccccgcc ggttgcagcg cgtgaccgca aatctccatc actttgtctt ttggcatgcc
    28561 ctgacgcatt gccaggccat aaatcgctac cagacaagca accagaatcg cggtaaacgg
    28621 atgaccaata aactcgaacc attcgtaagc ggtagagcct tccggcacaa aacgcgcggc
    28681 aatggttttc agccccacca gcaccagcgg cagcaggatc agcgacaggc tgaatccgaa
    28741 ggacggcatt ttgccttcgc cgagatgcgg ctcgctgatg tcgtcaggaa tatgcagctc
    28801 aacgtaacgg ctgatgaagt taccccacag cggcccggca ataatcattc ccggaattgc
    28861 cgcacacagg ccaatcagga tcatccagcc aaaatcggcg ttcatctgcg atgccagcag
    28921 cattggcgct ggccctggca ccaggaacgc cgccgctgcc gccacgcctg cgaataatgg
    28981 gattaccagc ttcaccaggt tcgtaccggt gtggcgcgcc attgagaaag caacactaat
    29041 cagcagaaca atcgccactt caaagaacag cggtagcgca cagaccagcc ccgcaaggcc
    29101 gatggcataa tgcgcgcggc tgtgaccgaa ggacttgagc attttgacgg caatctgatc
    29161 gactgcgccg gtttcatgta agatcttgcc aaacatagct cccagggcga caaccaccgc
    29221 caggaagccg agggtgcctc ccatcccttt ttccatcgtc gctgcgattt tatcgagcgg
    29281 cataccagaa aaaaggccag cccccatgga caccaccatc aaagccagga aagcgtgcat
    29341 acgcgccttc ataactaaaa acagcagcag taaaacagac cctactgctg ttaaaacaag
    29401 cgttaatgta gtcactactt atttgccttt tttaataacc tcaatggtgc ttgccacaac
    29461 accttccagc ggttgatcga tatctaccac cagtacatcg gtttcgtccg cacccggctc
    29521 ctgcagcgtt tcaaactgcg tcaccaacat ttgggtttta aagaaatggc ctttgcgcgc
    29581 tttcaggcgg ctttcaatca catcaaaatc gcctttcagg tagatgaaag agagattcgg
    29641 attaccttca cgcagcaagt cgcgatagtg ttttttcaat gcagaacaga cgatcagcga
    29701 cactttatta gtgcgctgca tagcaaacgc cgcgtcgttc agcgcctgca accacggttt
    29761 gcgatcgtcg tcattcagcg gttcgccaga cgccattttt tcgatattgc gccgtggatg
    29821 gaggaaatcg ccatcaagaa acgcggcatg aagttgatgc gccacttcac ttgcgaccgc
    29881 agatttgccg ctgcccgata cgcccatcaa gacgtaaatg tggtgatcat ggttagtcgt
    29941 gctcaaagtg gtgcccccac aatacaagaa ttaaaaatgt tacgggtaac tgttatcggt
    30001 aacattgtcc ggccggacaa tatcagaagc aatatccatc cgtgccttaa gtgtataagt
    30061 gtgagctact tcaaatttgt gggcttaaat agatccgccc ggtgacaagg tgaaacctaa
    30121 atctaacatt ttcggtgtca cagattcgcc acgaatacgc gccagcaggc gttcagcgcc
    30181 aatactgccc atccgctcac gcggcgtcag cacgctcgcc agtcgtggct ccatcacctg
    30241 accaatgtca tgaccgtgga aaccggcaat cgccatatcg tcaggaactt ttaaccccag
    30301 acgctgacat tcaaacgccg cgccgaccgc cagatcatca ttagtacaga acacgccatc
    30361 cagctgcgga tattcccgcc gcgcctggcg aatcagttca ataccggaag agtaagaaga
    30421 agattgctca accatcacgc tatacggcac caggcctgca tccagcatcg cctgttcgta
    30481 tcccttctgt ttgatgatag tacgttcgtc gagacgtgcg ccgagatagg caatgtggcg
    30541 atgcccgcgc gcaataatgg cggtagtcat ctggcgtgcc gcttcaaagt tatcaaaacc
    30601 gacggcgata tcgaggcatg gcgacttgct gtccatcagt tccaccacgg gaatccccgc
    30661 cacttcaatc atctttaagg tgcgcggcgt gtgcgtacgt tcagtgagga tcaggccgtc
    30721 gatattccag gagagcatgg attcgaggcg ttcttgctcc atttccggtt tataaccgta
    30781 gtgggccagc atggtctgat aaccgtgcgc gtcggtgacg ctttcgattc cgcgtaatac
    30841 ttccgcgaaa acctggttgg tgagagaagg taacaggacg ccaatcgccc ggctggtggc
    30901 gttagagagg atatcgggcg cacgattggg aatatagccc agttcatcaa gagccgcggc
    30961 aatcttgccg cgtagagcga cggaaacctg ctccgggttg cgtaaaaaac ggctgaccgt
    31021 cattttggtc acgcctacac ggtcagccac atcctgaagt acgggtcttt tctttttcat
    31081 cgtcctgaag gtacaaaaga gatagattct cttagtttaa cacggacgca tcagaacctt
    31141 cccggggaaa acagtgcttg tttacaatta ttagatatac actaaatcaa ttgcatcgca
    31201 ttgtgctaac gacgcggcaa aaccccggaa gcgtgcatca gcacgcgacc ggagcgagcg
    31261 aggaagcctg caaaaaggca ggcttcaaag atgccgttga gttttaaacc ggcggcagat
    31321 cgaacagtaa cacttcgctg tcgctatccg catggatgga gattgcctgc tcatcccaga
    31381 ttgccagacc atcgctggtc gaggctttca cgccgttaat ggtgacattg cctttcacca
    31441 cctggatcca gacgcggcgt tcagcggcaa tctgatgcac cgactgctca tctttcagca
    31501 acgcccagcg gtacagttcc atatcctgat gcactttcaa tgaaccatct cgcgcatccg
    31561 gcgagagcac cagctgtttg ccctgtacgg catcgaagcg acgctgttca taacgcggcg
    31621 taataccgtt ttcttcaggc atgatccaga tctgatacag atgcagacgc tcggtgctgc
    31681 ttgggttgta ctctgagtga cgaatacccg tacccgcact cataatctgg aactcacccg
    31741 ccggaacctg ctctttattg cccatgctgt cctggtgctc aacagtacct tccagcacgt
    31801 aggtcaaaat ttccatatct ttatgcgggt gagtgccgaa gccctgccct gcttcaatca
    31861 cgtcgtcgtt aatcacgcgc agcgcggaga agcccataaa gttcggatcg taatagttgg
    31921 caaaagagaa agtatgccag gagtccagcc agccatgatt tgcatgacca cgttcatttg
    31981 ctttgcgtaa gtagatcatt tgtattcgcc ccctgaatga tttcgatggg cttagtgtgg
    32041 acccgatctg cctgggatga tagagggtga aaattgaccc ctctgttcaa aaaaattgaa
    32101 ctattcgagg ggccatcaaa cttacttcgc gagagtcact gtggagggag aggcttgctc
    32161 gaatccgcgt tcaaggattt ccagattcgt aagaacttca gattccttga cgtaatttgg
    32221 cgcaccgtgg gtgattgttt gatacaacgc atcataaacg cgcccgtaat cgcccatctc
    32281 cggcttcatc tcttctctga ccgtcacgcc ctcgtcattg acatactcca gcacaccgac
    32341 cgaatcatcc gctgcgaatc ccggttcgcc cggcataata ttagccttca ggctggtttc
    32401 ctgctggtcg ataccgtatt taataaacga acctttctta ccgtgaacga taaatttcgg
    32461 ataatcgatt ttcaccagat ggctggtttt gacgatggct ttcaggtcgc cataaaacag
    32521 ttgcgcttcg aaagtatcgt caggattggc tttattgcgc aggctgcgga tgtcataagc
    32581 gacgtgatcc gggcgaccga acagagaaat aatctggtcc atcgtatgca caccaaggcc
    32641 atagaacgcg ccatcctgcg gcaacccagg tttggtttct gcgaccgggc ggtaatagtc
    32701 aaaatggctt tccacttcaa caatctctcc cagcttgccg ctttcaatcg cttttttcgc
    32761 cgtcaggaag caggaatcaa agcgacgatt ctgatatggc gtgacggtca gccctttgct
    32821 tttcgccagt gcaaacagct ctttcgcctg cgcaagtgtc ggagtgaacg gtttttcgac
    32881 cagcacattt ttcccggctt ccagcgcgcg cttcgcgtac tcgaagtggc tgtccgcatg
    32941 ggtgcagaca acaaccagct taacatcggg atcgtttagc acttcgttga gatcgctggt
    33001 gaaatggata tgagagtaaa tgggagcttg ttcttccggc tttgcatggc ggcgaaaaat
    33061 atgcgcgaca tgccagctat ccttgcggtt aagtacatac ggcagatggt aacgggtggt
    33121 gcttttgccg aagccaataa aggcgcagtt gatgaccatg atttcgtcct ttttaaggtg
    33181 gttatttaca ccttagcgca aagcggacgt ggttcctact ggagtgcgca taacgaacac
    33241 aagcacttcc cgagcataaa ttgagaacga aagatcaaaa aaaaagccag cacccggctg
    33301 gctaagtaat actggaagca atgtgagcaa tgtcgtgctt tcaggttctc cgcgagggtc
    33361 ttcctgatcg cgagacaata ataatcattc tcattcgcac ttgtccaaca ctttttgcaa
    33421 aaaaatgcat ttgactcgca ttcgaaagtc aatgatgttg aaagggacat ttaccccaaa
    33481 gaggacaaag gaatgagtga gatagtgata cgccatgctg aaacgcgcga ttacgaggcc
    33541 atcaggcaga ttcacgccca gccggaggtg tattacaaca cgctacaagt gcctcatcct
    33601 tccgattata tgtggcagga gcgactcgcc gatcgtcccg gcatcaagca actcgtcgcc
    33661 tgtattgatg gagacgtcgt gggccatctc accattgacg tgcaacagcg cccacgccgc
    33721 agtcatgttg ccgattttgg tatctgtgtc gactctcgtt ggaagaaccg cggcgtcgcc
    33781 agcgccctgg tgcgagagat gattgaaatg tgcgacaact ggttgcgggt agatcgtatc
    33841 gagctaacgg tgttcgtcga taacgcgcca gcaattaagg tctataaaaa attcggcttt
    33901 gagattgaag ggactggcaa gaagtacgca ttacgtaatg gtgaatatgt tgatgcgtat
    33961 tttatggcgc gggtgaagta atatagtgcc ctttttctga gatgggaaaa gggtgtcatt
    34021 aaaaa
//
LOCUS       DAPGOF010000036        33361 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.036, whole genome shotgun sequence.
ACCESSION   DAPGOF010000036 DAPGOF010000000
VERSION     DAPGOF010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.036"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..554)
                     /locus_tag="RHV13_001471"
     CDS             complement(<1..554)
                     /locus_tag="RHV13_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HDV1241024.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPAPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQ
                     ALPEELRLSPHRYLATNSPQGPWWL"
     gene            complement(714..926)
                     /gene="rpmE"
                     /locus_tag="RHV13_001472"
     CDS             complement(714..926)
                     /gene="rpmE"
                     /locus_tag="RHV13_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HDV1241025.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            1129..3327
                     /gene="priA"
                     /locus_tag="RHV13_001473"
     CDS             1129..3327
                     /gene="priA"
                     /locus_tag="RHV13_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDV1241026.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3483..4508
                     /gene="cytR"
                     /locus_tag="RHV13_001474"
     CDS             3483..4508
                     /gene="cytR"
                     /locus_tag="RHV13_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HDV1241027.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAATHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4600..5559
                     /gene="ftsN"
                     /locus_tag="RHV13_001475"
     CDS             4600..5559
                     /gene="ftsN"
                     /locus_tag="RHV13_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HDV1241028.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVTRAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5652..6182
                     /gene="hslV"
                     /locus_tag="RHV13_001476"
     CDS             5652..6182
                     /gene="hslV"
                     /locus_tag="RHV13_001476"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HDV1241029.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            6192..7523
                     /gene="hslU"
                     /locus_tag="RHV13_001477"
     CDS             6192..7523
                     /gene="hslU"
                     /locus_tag="RHV13_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HDV1241030.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7590..8516
                     /gene="menA"
                     /locus_tag="RHV13_001478"
     CDS             7590..8516
                     /gene="menA"
                     /locus_tag="RHV13_001478"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDV1241031.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8609..9094
                     /gene="rraA"
                     /locus_tag="RHV13_001479"
     CDS             8609..9094
                     /gene="rraA"
                     /locus_tag="RHV13_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HDV1241032.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(9179..9424)
                     /gene="zapB"
                     /locus_tag="RHV13_001480"
     CDS             complement(9179..9424)
                     /gene="zapB"
                     /locus_tag="RHV13_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HDV1241033.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9850..10695
                     /gene="glpF"
                     /locus_tag="RHV13_001481"
     CDS             9850..10695
                     /gene="glpF"
                     /locus_tag="RHV13_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HDV1241034.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10718..12226
                     /gene="glpK"
                     /locus_tag="RHV13_001482"
     CDS             10718..12226
                     /gene="glpK"
                     /locus_tag="RHV13_001482"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDV1241035.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            12456..13466
                     /gene="glpX"
                     /locus_tag="RHV13_001483"
     CDS             12456..13466
                     /gene="glpX"
                     /locus_tag="RHV13_001483"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HDV1241036.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGAIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13563..14309
                     /gene="fpr"
                     /locus_tag="RHV13_001484"
     CDS             13563..14309
                     /gene="fpr"
                     /locus_tag="RHV13_001484"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HDV1241037.1"
                     /translation="MADWVTGKVTKVQNWTEALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALRPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAVGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(14314..14742)
                     /gene="uspD"
                     /locus_tag="RHV13_001485"
     CDS             complement(14314..14742)
                     /gene="uspD"
                     /locus_tag="RHV13_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HDV1241038.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14769..15068)
                     /locus_tag="RHV13_001486"
     CDS             complement(14769..15068)
                     /locus_tag="RHV13_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HDV1241039.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCISKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(15280..15720)
                     /locus_tag="RHV13_001487"
     CDS             complement(15280..15720)
                     /locus_tag="RHV13_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDV1241040.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15821..16420
                     /locus_tag="RHV13_001488"
     CDS             15821..16420
                     /locus_tag="RHV13_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HDV1241041.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            16528..17295
                     /gene="tpiA"
                     /locus_tag="RHV13_001489"
     CDS             16528..17295
                     /gene="tpiA"
                     /locus_tag="RHV13_001489"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDV1241042.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(17350..18105)
                     /gene="cdh"
                     /locus_tag="RHV13_001490"
     CDS             complement(17350..18105)
                     /gene="cdh"
                     /locus_tag="RHV13_001490"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HDV1241043.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(18212..19201)
                     /gene="sbp"
                     /locus_tag="RHV13_001491"
     CDS             complement(18212..19201)
                     /gene="sbp"
                     /locus_tag="RHV13_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HDV1241044.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(19521..20483)
                     /gene="pfkA"
                     /locus_tag="RHV13_001492"
     CDS             complement(19521..20483)
                     /gene="pfkA"
                     /locus_tag="RHV13_001492"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDV1241045.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20664..21566)
                     /gene="fieF"
                     /locus_tag="RHV13_001493"
     CDS             complement(20664..21566)
                     /gene="fieF"
                     /locus_tag="RHV13_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HDV1241046.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21715..22215)
                     /gene="cpxP"
                     /locus_tag="RHV13_001494"
     CDS             complement(21715..22215)
                     /gene="cpxP"
                     /locus_tag="RHV13_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HDV1241047.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            22365..23063
                     /gene="cpxR"
                     /locus_tag="RHV13_001495"
     CDS             22365..23063
                     /gene="cpxR"
                     /locus_tag="RHV13_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HDV1241048.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            23060..24433
                     /gene="cpxA"
                     /locus_tag="RHV13_001496"
     CDS             23060..24433
                     /gene="cpxA"
                     /locus_tag="RHV13_001496"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HDV1241049.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETLKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(24539..25213)
                     /gene="yiiM"
                     /locus_tag="RHV13_001497"
     CDS             complement(24539..25213)
                     /gene="yiiM"
                     /locus_tag="RHV13_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HDV1241050.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(25362..26345)
                     /gene="kdgT"
                     /locus_tag="RHV13_001498"
     CDS             complement(25362..26345)
                     /gene="kdgT"
                     /locus_tag="RHV13_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HDV1241051.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(26605..27225)
                     /gene="sodA"
                     /locus_tag="RHV13_001499"
     CDS             complement(26605..27225)
                     /gene="sodA"
                     /locus_tag="RHV13_001499"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HDV1241052.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            27510..28544
                     /gene="rhaT"
                     /locus_tag="RHV13_001500"
     CDS             27510..28544
                     /gene="rhaT"
                     /locus_tag="RHV13_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HDV1241053.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNLLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(28541..29407)
                     /gene="rhaR"
                     /locus_tag="RHV13_001501"
     CDS             complement(28541..29407)
                     /gene="rhaR"
                     /locus_tag="RHV13_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HDV1241054.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSVSAGQPHWRLGSMGMAQARQIIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(29463..30299)
                     /gene="rhaS"
                     /locus_tag="RHV13_001502"
     CDS             complement(29463..30299)
                     /gene="rhaS"
                     /locus_tag="RHV13_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HDV1241055.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQEQDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            30587..32056
                     /gene="rhaB"
                     /locus_tag="RHV13_001503"
     CDS             30587..32056
                     /gene="rhaB"
                     /locus_tag="RHV13_001503"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HDV1241056.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESKTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQVLPACRFIINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            32053..33312
                     /gene="rhaA"
                     /locus_tag="RHV13_001504"
     CDS             32053..33312
                     /gene="rhaA"
                     /locus_tag="RHV13_001504"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HDV1241057.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSIHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYLGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
BASE COUNT         8141 a         8655 c         8675 g         7890 t
ORIGIN      
        1 agccaccacg gcccctgcgg actgtttgtc gccagataac gatgcggact taagcggagt
       61 tcttccggca gcgcctgcca gagtgcggcc agccggtgac cttcatccag tttcgccacg
      121 ccgccgcgca ccagccacag tgactcgctg cggctgtaac cgtcctcacc gggaaacagg
      181 tgctcaaaat acaggcttct gccgccgtta tcactgagta tcagtgtgtt atcgcgcagc
      241 tgtaagcgga tatccgcagg cattttccag ccggggccga ggctccccac cggcgcgggc
      301 gtttttgtcc ggtaactgct gtaggtgcgg gagagaatga acggcagcgg ggcgggcagg
      361 gcgatgtcgg tttcaccggg aaggaccttt gcaccgagca ggggattgac cggatggccg
      421 gacgtcaccc cgccggggca cactgaacag gccacgccgg tgggggcacc aatgcgcacc
      481 ccggctgaac cctgaacaat gctaccgcca tactgcgtca tgtcgccctg acgcgccgcc
      541 ggttttccgc tcatcgtaaa atccttttga aataaataag aaccagaagc ctagcgccag
      601 tcaacaacag gaaatgataa tgagttaaca ccccggaatt tttcagaatt tataaaaggc
      661 aaattacagg cacaaaaaaa gcgccgtgcg gcgctttttt cggaaatccg gtcttatttg
      721 ctgcccggga tgttgaaacg cttgttgaag cggtcaacac ggccaccggt agcaacatca
      781 cgctgtttgc cagtgaagaa cgggtggcac ttgctgcaca cgtcgaggtt caggtcatga
      841 ccaacggtgg agcggatttt cattacgtta ccgcaagagc agctagcagt aatttcttcg
      901 tatttcgggt gaatatcttt tttcatggga aaacctcggt ttaaggccgc gtcgctcttc
      961 cagccctaac gccagacacc acgcgatgtt aaaagtatag cttcaatacg atcatttcgt
     1021 acgaaggcgc gaaatcatac agaaattaac cagcgtatgc aaactgatcc gcactcttct
     1081 acggcaatgt gtatactaac ccaccgaatt tcaagtcagg atgatgctat gcccgttgcc
     1141 cacgttgcct tgcccgttcc gcttcctcgt acctttgact atctgctgcc agaaggcatg
     1201 acggttaaag ccgggtgtcg cgtgcgcgtg ccgtttggca aacagcagga gcgcatcggg
     1261 gttgtggtgt cagttagcga tgtcagtgag ctgccgctta acgagctgaa agcggtagtg
     1321 gaagtgctgg acgttgagcc ggtatttact cactccgtct ggcggttact gctctgggcg
     1381 gcagattact atcatcatcc gattggcgat gtgctgtttc atgccttgcc gattttactg
     1441 cgccaggggc ggcctgcggc gaacgcgccg atgtggtact ggtttgccac tgaacaaggc
     1501 caggcggtgg atctgaacag cctgaaacgc tcccccaagc aacaacaggc gctggcagcg
     1561 ttacggcaag gcaaaatctg gcgcgaccag gtcgccacgc tcgaatttaa tgatgccgcg
     1621 ttgcaggcgc tacgcaaaaa aggtctgtgt gatttagcaa gtgaaacacc agagtttagc
     1681 gactggcgaa cgaactatgc cgtttctggt gagcggttgc gattgaatac cgaacaggcc
     1741 accgccgttg gcgcaattca tagcgcggca gatacttttt ctgcctggct gctggcgggc
     1801 gttaccggtt ccggtaaaac ggaggtttat ctcagtgtac tggaaaacgt gctcgctcag
     1861 ggcaaacagg cgctggtgat ggtgccggaa atcggcctga caccgcaaac tatcgcccgt
     1921 tttcgtgaac ggtttaatgc gcccgtggaa gttctgcatt ccggtctgaa cgacagcgag
     1981 cgtctttcgg cgtggttgaa agcaaaaaat ggcgaggcgg cgattgtgat cggcacccgc
     2041 tccgcgctgt ttacgccgtt taaaaatctc ggcgtgattg tcattgatga agagcacgac
     2101 agctcctaca agcagcagga aggctggcgc tatcatgccc gcgacctggc ggtgtatcgt
     2161 gcgcacagcg agcaaatccc gattattctt ggctccgcaa cgcccgcgct ggaaacgtta
     2221 tgcaacgtcc agcagaaaaa ataccgcctg ctgcgcctga cccgtcgggc ggggaatgcg
     2281 cgcccggcaa ttcaacatgt gctggattta aaaggccaga aagtgcaggc ggggctggct
     2341 ccggcgttaa tcacccgtat gcgccagcat ttacaggcca acaatcaggt tatcctcttt
     2401 cttaatcgtc ggggttttgc gcctgcgctg ctgtgccacg actgcggctg gatagccgaa
     2461 tgcccacgtt gcgatcacta ttacacgctg catcaggcgc aacagcattt gcgctgtcac
     2521 cactgtgaca gtcagcgtcc agtaccgcgc cagtgccctt cctgcggttc cacgcacctg
     2581 gtacccgtgg ggctgggcac cgaacagctt gaacagacgc ttgcgccgct gttccctggt
     2641 gtgcccattt ctcgtatcga ccgagatacc accagccgca aaggggcgct ggaacaacaa
     2701 ctggcggaag tacatcgcgg cggtgcgagg attttgatcg gcacacaaat gctggcgaaa
     2761 ggtcaccact ttccggatgt gacgctggtg gcgttactgg atgtggacgg cgcgctgttt
     2821 tctgccgatt ttcgctcggc agagcgtttc gctcagcttt acacccaggt cgctggtcgt
     2881 gccgggcgtg cgggtaaaca aggcgaagtg gtgctgcaaa cgcaccatcc ggaacatcct
     2941 ctgttgcaaa cgttgctcta taaaggctac gacgcctttg ccgaacaggc actggctgag
     3001 cgacgcatga tgcagctgcc gccgtggacc agccatgtga ttgtgcgtgc ggaagatcat
     3061 aacaatcagc atgcgccgct gttcctgcaa caactgcgta atctgatcct ctccagcccg
     3121 ctggcagacg acaaattgtg ggttctcggt ccggttccgg cactggcacc taaacgtggc
     3181 ggtcgctggc gctggcagat attgttgcag cacccttccc gcgtgcgctt gcaacacatc
     3241 attaacggta cgctggcact catcaataca ataccggatt cccgcaaggt gaaatgggta
     3301 ctggatgttg atccgattga gggttaaacc gctcacgatg cgaggcggat cgaaaaattc
     3361 aatattcatc acacttttca tgaaaattct gtaaccgttt tcacgcgcta tctgctaaaa
     3421 atgttgccga tgtgaagtaa acatggatgt agtacgcctg acgtgccagg cgaggagtga
     3481 gtgtgaaagc gaagaagcag gaaactgccg cgaccatgaa agacgttgcc ctcaaggcaa
     3541 aagtctctac agcgactgtc tcccgagcat taatgaatcc cgataaagtc tcccaggcca
     3601 cccgtaatcg ggttgaaaaa gcggcccggg aagtgggtta tttaccgcag cctatggggc
     3661 gcaacgtcaa gcgtaatgaa tcccgcacca ttctggtgat tgtcccggat atctgcgatc
     3721 ccttctttag cgaaattatt cgcggtatcg aagttacggc agcaactcac ggatatctgg
     3781 tgctgattgg cgactgtgcg catcaaaatc agcaggaaaa aacctttatc gatttgatca
     3841 tcaccaagca aattgatggc atgttgctgc tgggttcaag gctgccgttt gatgccagca
     3901 ttgaggaaca gcgtaatctg ccgccgatgg tgatggcgaa cgaatttgca ccggaactgg
     3961 agctgcctac cgttcatatc gacaatctga ccgccgcatt tgatgcagta aattatttat
     4021 atgagcaagg gcataaacgg attggctgta tagccggtcc cgaagagatg ccgctgtgtc
     4081 actaccgcct gcaaggttac gttcaggcgc tgcgtcgctg cggcattatg gttgatccgc
     4141 aatacatcgc ccgtggcgac ttcaccttcg aagccggaag caaagcaatg caacagctgc
     4201 ttgatcttcc acaaccgcct actgctgtct tctgccatag cgatgtaatg gcactcggcg
     4261 cactttcgca ggcaaaacgc caggggctga aagtaccgga agacctttcc ataatcggtt
     4321 ttgataacat cgacctgacg caattttgtg atccgccgct gacaaccatc gcgcagccac
     4381 gttacgaaat cggtcgggaa gctatgctgt tattgcttga tcaaatgcag gggcaacacg
     4441 ttggcagtgg ctctcgttta atggactgcg aacttatcat ccggggatca acacgcgcgt
     4501 taccttaaag taaaccttta agactgccgt gactggtcaa aggcccgccg cttaagtaac
     4561 atggcgggct gacgaacgaa taaatacagc gaaacgatag tggcacaacg agattatgta
     4621 cgccgcagcc aaccggcacc ttcgcggcga aaaaagagca cctcacggaa aaagcaacga
     4681 aatctgcctg cggtttctcc cgctatggtc gctattgctg ccgccgttct tgtgaccttt
     4741 atcggtggtc tgtacttcat tacgcatcac aagaaagaag agtccgagac gctgcaaagc
     4801 cagaaagtga ccggaaacgg actaccacca aaaccagaag aacgctggcg ctacatcaaa
     4861 gagctggaaa gtcgccagcc gggtgtgcgt gcgcccacag aaccttctgc tggtggtgaa
     4921 gtgaaaacgc cggagcaact gacaccagaa caacgtcagc ttcttgaaca aatgcaggct
     4981 gatatgcgcc agcagccaac gcagctggtt gaggttccgt ggaatgaaca gacgccggaa
     5041 cagcgtcagc aaacgctaca gcgccaacgt caggcgcagc aattagcgga acagcaacgc
     5101 ctggctcagc agtcgcgtac gactgaacaa agctggcagc agcagacgcg tacgtcgcaa
     5161 gccgcgccgg tacaggcgca accgcgccag tctaaaccgg cttctactca gcagccgtac
     5221 caggatctgc tgcaaacccc tgcgcacacg accgcgcagt cgaagccgca gcaagctgcg
     5281 ccggttactc gtgccgctga cgcgccaaaa ccgacggcgg agaaaaaaga cgaacgccgc
     5341 tggatggtgc agtgcggttc gttcagaggc gcggaacagg cagagacggt acgtgctcag
     5401 ctggcgttcg aaggatttga ctcgaaaatc accaccaaca atggctggaa tcgtgtggtc
     5461 attggcccgg tgaaaggcaa agagaacgca gacagcaccc tcaatcggtt gaagatggcg
     5521 ggtcatacaa actgcattcg gctcgccgcc gggggttgaa accctcaaaa tcccccccat
     5581 ctataattgc attatgcccc gtacttttgt acggggtttg tactctgtat tcgtaaccaa
     5641 ggggtcagct cgtgacaact atagtaagcg tacgccgtaa cggccatgtg gtcatcgctg
     5701 gtgatggtca ggccacgttg ggcaataccg taatgaaagg caacgtgaaa aaggtccgcc
     5761 gtctgtacaa cgacaaagtc atcgcgggct ttgcgggcgg tactgcggat gcttttacgc
     5821 tgttcgaact gtttgaacgt aaactggaaa tgcatcaggg ccatctggtc aaagccgccg
     5881 ttgagctggc gaaagactgg cgtaccgatc gcatgctgcg caaacttgaa gcactgctgg
     5941 cagtcgcgga tgaaactgca tcgcttatca tcaccggtaa cggtgacgtg gtgcagccag
     6001 aaaacgatct tattgctatc ggctccggcg gcccttacgc ccaggctgcg gcgcgtgcac
     6061 tgttagaaaa tactgaactg agtgctcgtg aaattgctga aaaggcgttg gatattgcag
     6121 gcgacatttg catctatacc aaccatttcc acaccatcga agaattaagc tacaaagcgt
     6181 aaggatctcc catgtctgaa atgaccccac gcgaaatcgt cagcgaactg gataagcaca
     6241 tcatcggcca ggacaacgcc aagcgttctg tggcgattgc tctgcgtaac cgctggcgtc
     6301 gcatgcagct caacgaagag ctgcgccatg aagtgacccc gaaaaatatc ctgatgatcg
     6361 gcccgaccgg tgtcggtaaa actgaaatcg cccgtcgtct ggctaagctg gcgaatgcgc
     6421 cgttcatcaa agttgaagcg accaaattca ccgaagtggg ctacgtcggt aaggaagtgg
     6481 attctattat tcgcgatctg accgatgccg ccgtgaaaat ggtacgcgtc caggctatcg
     6541 agaaaaaccg ttatcgcgct gaagaactgg cagaagaacg tattctcgac gtgctgatcc
     6601 caccggctaa aaacaactgg ggacagaccg aacagcagca ggaaccgtcc gctgctcgtc
     6661 aggcattccg caaaaaactg cgtgaaggcc agcttgatga caaagaaatc gagatcgatc
     6721 ttgccgcagc accgatgggc gttgaaatta tggctcctcc aggcatggaa gagatgacca
     6781 gccagctgca gtctatgttc cagaacctgg gcggtcagaa gcaaaaagcg cgtaagctga
     6841 aaatcaaaga cgccatgaag ctgctgattg aagaagaagc ggcgaaactg gtgaacccgg
     6901 aagagctgaa gcaagacgct atcgacgctg ttgagcagca cgggatcgtg tttatcgacg
     6961 aaatcgacaa aatctgtaag cgcggcgagt cttccggtcc ggatgtttct cgtgaaggcg
     7021 ttcagcgaga cctgctgccg ctggtagaag gttgcaccgt ttccaccaaa cacgggatgg
     7081 tcaaaactga ccacattctg tttatcgctt ctggcgcgtt ccagattgcg aaaccgtctg
     7141 acctgatccc ggaactgcaa ggtcgactgc caatccgcgt tgaactgcag gcactgacca
     7201 ccagcgactt cgagcgtatt ctgaccgagc cgaatgcctc tatcaccgtg cagtacaaag
     7261 cactgatggc gactgaaggc gtaaatatcg agtttaccga ctccggtatt aaacgcatcg
     7321 cggaagcggc atggcaggtg aacgaatcta ccgaaaacat cggtgctcgt cgtttacaca
     7381 ctgttctgga gcgtttaatg gaagagattt cctacgacgc cagcgattta agcggtcaaa
     7441 atatcactat tgacgcagat tatgtgagca aacatctgga tgcgttggtg gcagatgaag
     7501 atctgagccg ttttatccta taatcgcgtt caatcatttt catcattgtt tgatggggct
     7561 gaaaggcccc atttttattg gcgcgtatta tgactgaaca acaaattagc cgaactcagg
     7621 cgtggctgga aagtttacga cctaaaaccc tccccctcgc ctttgctgca attatcgtcg
     7681 ggacggcgct ggcatggtgg caaggtcatt ttgatccgct ggtcgccctg ctggcactga
     7741 ttaccgccgg gctattacag atcctttcta acctcgccaa tgattacggc gatgcagtaa
     7801 aaggcagcga taaacctgac cgcattgggc cgctacgcgg catgcaaaaa ggggtcatta
     7861 cccagcaaga gatgaaacgg gcgctcatta ttaccgtcgt gctcatctgt ctctccgggc
     7921 tggcactggt tgcagtggca tgccatacgc tggctgattt tgtcggtttc ctgattcttg
     7981 gcgggttgtc gatcattgcc gcgatcacct acaccgtggg caatcgtcct tatggttata
     8041 tcggtctggg tgatatttcc gtactggttt tctttggctg gttaagcgtc atggggagct
     8101 ggtatttaca ggctcataca ttgattccgg cactgatcct tccggcgacc gcctgtggcc
     8161 tgctggcaac ggcagtactg aacattaata acctgcgtga tatcaatagc gaccgcgaaa
     8221 atggcaaaaa cacgctggtg gtgcgcttag gtgaagtgaa cgcgcgtcgt tatcatgcct
     8281 gcctgctgat gggctctctg gtatgtctgg cgctgtttaa tctcttttcg ctgcatagcc
     8341 tgtggggctg gctgttcctg ctggcggcac cattactggt gaagcaagcc cgttatgtga
     8401 tgcgggaaat ggacccggtg gcgatgcgac caatgctgga acgtactgtc aagggagcgt
     8461 tactgactaa cctgctgttt gttttaggga tattcctaag ccagtgggca gcataactga
     8521 caaatatcaa ttaacaattg atgattttgc caacagccta catagcgcga tatactgaaa
     8581 attctcgcag caactgaatg ttaagcctat gaaatacgat acttccgagc tttgtgacat
     8641 ctatcaagaa gatgttaacg tcgtggaacc gctgttctcc aactttggcg gacgggcgtc
     8701 gtttggcgga caaataatca cggtaaaatg tttcgaggac aacgggttgc tgtacgatct
     8761 gctcgaacag aatggccgtg gtcgtgttct tgtcgtcgat ggcggtggtt ctgttcgtcg
     8821 cgcactggtc gatgctgaac tggcgcgtct ggcagtacaa aatgaatggg aaggtctggt
     8881 tatttacggc gcggtgcgtc aggtagatga cctggaagag ttggatatcg gcatccaggc
     8941 gatggcggca attccggttg gtgccgctgg cgaaggcatt ggcgaaagcg atgtccgcgt
     9001 caattttggc ggtgtaacct tcttctccgg cgaccatctt tatgccgaca ataccgggat
     9061 tattctttca gaagatccgc tggatattga atgataagaa aggcaccgca aggtgccttt
     9121 tttctgcgtt acctgttggc ctacacagta aagaaattac gcgaaagatg aagcgtaatc
     9181 agacctcttc catgcgaccc agcagggcct gcagacgttc ctgccagccg ttctgctgtt
     9241 ctttcagatg gttgttctca cgctccagct cttcgcgctg atgctgggca ttttgaactt
     9301 cctgcgacag tgagttgttt ttttctttca gctcttcgat ttccatctgc aacagagtga
     9361 tggtatcaat cgcctgctgt acttttgctt ccagtttctc aaacacttct aatgacattg
     9421 tcatacctct cctgaattgc aaggcgttga tggataaaaa atcctcgtcc cgattaccgg
     9481 tgacgcctta ataaatacga gcgcacttta gttagctccg attgtatgaa gccgcgccat
     9541 cgctgtccag cggcacgcct tgcagattac ggtttgccac acttttcatc cttctcctag
     9601 tgacataatc cacatcaatc gaaaatgtta ataaatttgt tgcgcgaatg atctaacaaa
     9661 catgcatcat gtacaatcag atggaataaa tggcgcgata acgctcattt tatgacgaag
     9721 cacacacatt ttaagttcga tatttctcgt ttttgctcgt taacgataag tttacagcat
     9781 gcctacaagc atcgtggagg tccgtgactt tcacgcatac aacaaacatt aactcttcag
     9841 gatccgatta tgagtcaaac atcaaccttg aaaggccagt gcattgctga attcctcggt
     9901 accgggttgt tgattttttt cggtgtgggt tgcgttgcag cactaaaagt cgctggtgcg
     9961 tcttttggtc agtgggaaat cagtgtcatt tggggactgg gggtggcaat ggccatctac
    10021 ctgaccgcag gggtttccgg cgcgcatctt aatcccgctg ttaccattgc attgtggctg
    10081 tttgcctgtt tcgacaagcg caaagttatt ccttttatcg tttcacaagt tgccggcgct
    10141 ttctgcgctg cggctttagt ttacgggctt tactacaatt tatttttcga cttcgagcag
    10201 actcatcata ttgttcgcgg cagcgttgaa agtgttgatc tggctggcac tttctctact
    10261 taccctaatc ctcatatcaa ttttgtgcag gctttcgcag ttgagatggt gattaccgct
    10321 attctgatgg ggctgatcct ggcgttaacg gacgatggca acggtgtacc acgcggccct
    10381 ttggctccct tgctgattgg tctactgatt gcggtcattg gcgcatctat gggcccattg
    10441 acgggttttg ccatgaaccc agcgcgtgac ttcggtccga aagtctttgc ctggctggcg
    10501 ggctggggca atgtcgcctt taccggcggc agagacattc cttacttcct ggtgccgctt
    10561 tttggcccta tcgttggcgc gattgtaggt gcatttgcct accgcaaact gattggtcgc
    10621 catttgcctt gcgatatctg tgttgtggaa gaaaaggaga ccacaactcc ttcagaacaa
    10681 aaagcttcgc tgtaatatga ctacgggaca attaaacatg actgaaaaaa aatatatcgt
    10741 tgcgctcgac cagggcacca ccagctcccg cgcggtcgta atggatcacg atgccaatat
    10801 cattagcgtg tcgcagcgcg aatttgagca aatctaccca aaaccaggct gggtagaaca
    10861 cgacccaatg gaaatctggg ccacccaaag ctccacgctg gtagaagtgc tggcgaaagc
    10921 cgatatcagt tccgatcaaa ttgcagctat cggtattacg aaccagcgtg aaaccactat
    10981 tgtctgggaa aaagaaaccg gcaagcctat ctataacgcc attgtctggc agtgccgtcg
    11041 taccgcagaa atctgcgagc atttaaaacg tgacggttta gaagattata tccgcagcaa
    11101 taccggtctg gtgattgacc cgtacttttc tggcaccaaa gtgaagtgga tcctcgacca
    11161 tgtggaaggc tctcgcgagc gtgcacgtcg tggtgaattg ctgtttggta cggttgatac
    11221 gtggcttatc tggaaaatga ctcagggccg tgtccatgtg accgattaca ccaacgcctc
    11281 tcgtaccatg ttgttcaaca tccataccct ggactgggac gacaaaatgc tggaggtgct
    11341 ggatattccg cgcgagatgc tgccagaagt gcgtcgttct tccgaagtgt acggtcagac
    11401 taacattggc ggcaaaggcg gcacgcgtat tccaatctcc gggatcgccg gtgaccagca
    11461 ggccgcgctg tttggtcagt tgtgcgtgaa agaagggatg gcgaagaaca cctatggcac
    11521 tggctgcttt atgctgatga acactggcga gaaagcggtg aaatcagaaa acggcctgct
    11581 gaccaccatc gcctgcggcc cgactggcga agtgaactat gcgttggaag gtgcggtgtt
    11641 tatggcaggc gcatccattc agtggctgcg cgatgagatg aagttgatta acgacgccta
    11701 cgattccgaa tatttcgcca ccaaagtgca aaacaccaat ggtgtgtatg tggttccggc
    11761 atttaccggg ctgggtgcgc cgtactggga cccgtatgcg cgcggggcga ttttcggtct
    11821 gactcgtggg gtgaacgcta accacattat acgcgcgacg ctggagtcta ttgcttatca
    11881 gacgcgtgac gtgctggaag cgatgcaggc cgactctggt atccgtctgc acgccctgcg
    11941 cgtggatggt ggcgcagtag caaacaattt cctgatgcaa ttccagtccg atattctcgg
    12001 cacccgcgtt gagcgcccgg aagttcgcga agtgactgca ttgggtgcgg cctatctcgc
    12061 aggcctggcg gttggcttct ggcagaacct cgacgagctg caagagaaag cggtgattga
    12121 gcgcgagttc cgtccaggca tcgaaaccac tgagcgtaat taccgttacg caggctggaa
    12181 aaaagcggtc aaacgcgcga tggcgtggga agaacacgac gagtaatgtc aggcacctga
    12241 atcgattgcc tgatgctgat cgcctgatgc gacgcttacg cgtcttatca ggcctacgag
    12301 ccaattccat tttgtgtagg cgggataagg cgttcacgcc gcatccggca tcacgcagac
    12361 tctgaaaccg tgcgggggca accccgcaca catcaataat cccacccttc ccctgtgcta
    12421 cacttcgcgc cattccttac tgcttagagt ttgctatgag acgagaactt gccatcgaat
    12481 tttcccgcgt caccgaatct gcggcgctgg ctggctacaa atggttagga cgcggcgata
    12541 aaaacaccgc ggacggcgcg gcggtaaacg ccatgcgtat tatgctcaac caggtcaaca
    12601 ttgacggcac catcgtcatt ggtgaaggtg aaatcgacga agcaccgatg ctctacattg
    12661 gtgaaaaagt cggtactggt cgcggcgacg cggtagatat tgctgttgat ccgattgaag
    12721 gcacgcgcat gacggcgatg ggtcaggcta acgcgctggc ggtgctggca gtaggcgata
    12781 aaggctgctt cctcaacgcg ccagatatgt atatggagaa gctgatcgtc ggaccgggag
    12841 ctaagggcgc cattgacctg aatctgccgt tggcggataa cctacgcaat gttgccgcgg
    12901 cgctcggaaa accattgagc gaactaacgg taacgattct ggcaaaaccg cgacacgatg
    12961 ccgttatcgc agaaatgcag caactcggcg tacgcgtatt tgctattccg gatggcgacg
    13021 ttgcggcctc aattctcacc tgtatgccag acagcgaagt tgacgtgctg tacggtattg
    13081 gtggcgcgcc ggaaggcgtg gtttctgcgg cggtgatccg cgcattagat ggcgacatga
    13141 acggtcgtct gctggcgcgt catgacgtca aaggcgacaa cgaagagaat cgtcgcattg
    13201 gcgagcagga gctggcacgc tgcaaagcga tgggcatcga agccggtaaa gtattgcgtc
    13261 tgggcgatat ggcgcgcagc gataacgtca tcttctctgc caccggtatt accaaaggcg
    13321 atctgctgga aggcattagc cgcaaaggca atatcgcgac taccgaaacg ctgctgatcc
    13381 gcggcaaatc acgcaccatt cgccgcattc agtctatcca ctatctggat cgcaaagacc
    13441 cggaaatgca ggtgcacatc ctctgattga tttgatcgat tgagccttcc agtccttcgg
    13501 gactggaatt tttttgttcg gagaacgaag ataaggcaag tcaatcaaaa caggagaaaa
    13561 acatggctga ttgggtaaca ggcaaagtca ctaaagtgca gaactggacc gaagccctgt
    13621 ttagtctcac cgttcacgct cccgtgcttc cgtttaccgc cgggcaattc actaaacttg
    13681 gccttgaaat tgacggcgaa cgcgtccagc gcgcctactc ctatgttaac tcgcccgata
    13741 atcccgatct ggagttttac ctggtcaccg tccccgatgg caaattaagc ccacgactgg
    13801 cggcactgag accgggcgat gaagtgcagg ttgttagcga agcggcagga ttctttgtgc
    13861 tcgatgaagt gccagattgc gaaacgctat ggatgctggc aaccggtaca gcgattggcc
    13921 cttatttatc gattctacaa ctaggcaaag atttagatcg cttcaaaaat ctggtcctgg
    13981 tgcacgccgc acgttatgcc gccgacttaa gctatttgcc actgatgcaa gaactggaaa
    14041 aacgctacga aggaaaactg cgcattcaga cggtggtcag tcgggaaacg gcagcgggtt
    14101 cgctcaccgg acggataccg gcgttaattg aaagtggtga actggaaagc gcggttggcc
    14161 tgccgatgaa taaagaaacc agccatgtga tgctgtgcgg caatccacag atggtgcgcg
    14221 atacacaaca gttgctgaaa gagacccggc agatgacgaa acatttacgt cgccgaccgg
    14281 gccatatgac agcggagcat tactggtaag cggttactta tcgataaacg gcacgatgag
    14341 caaatccgca ctcatcttat tgatcatccc gcgatatgcc ggcatcaaac ggttgataaa
    14401 tgagtgatga tgaccacaga caaggaggtc gcactgctct ttttgcataa tttccagcag
    14461 tgtttccggc atttctccgc gttcaatacg cagttttgtc ttcggccatt gaatattttt
    14521 cgtcagttta tacagcttgt tatccgactt attcttcaac aattgaagaa tatcttctgt
    14581 tgcagggaag tagatacccg ggtacaactc gcttaagcca tcatcaatat gaattaacgt
    14641 caggtgagcg tcattatgtc tggcgagctc cagggcttta ttcaccagta aggcatcttc
    14701 ttcattcccg gaaattgcca cgccaatgtg tttataagcc atgtttaact ccttctaaag
    14761 caactccatc aagctaacga acgcagtgat aactcaaaaa taatcatctc ggcctggcat
    14821 gagaaggtga aggccgcatc aagctcaact tgcccctcca gatccttaat cttataggcc
    14881 acgctggccc cttcatctgc agaggatgca gcggcggcaa gctctttcag tttggtcata
    14941 aaggactctg cttcttcgcg ggtggcaaaa aagcgcgaaa acttactgat acagttgtcg
    15001 ttatcaatca ccgtaccgat atcaatcgca catcctttag tactgcattt atctacgaca
    15061 tctttcatag ggcacctctg tatattcgcc tctctgtcga ttacgactta agaataatgc
    15121 gccgttaagg gaagaacaaa caagtaatga tcttatggat taccattttt tttgatattg
    15181 cttaaggtga agagttcaga aggattttcc gggtattgtc atttgcatcg tggaaataac
    15241 aatcccggaa aaaaatagca gtaagagata tattaaaggt taattacttg atttattgtc
    15301 ggctttatac ttcacatcct gagtatcttt accatattta ttttcgcctt gtgtgccaac
    15361 aaacgcacca agatcgataa gcatcatcac cagaatcaac gtcgggacaa aacgccccac
    15421 cgcccattgc cagacacccg gtaaaatcgc ccagttaccc gccagtagca tccacgccac
    15481 aatcatcaga aatgcccatg ctccggaacg cccgcgatca tgcaagcgct taacggttac
    15541 tgccgctgtt ggccagagca agcacacaag gcaaaacgcc gcggtctgaa tatcgagtaa
    15601 atttttaccc gccagtgaga aaagcaccag catgcctgcg aaccacaggc ctatccaaat
    15661 ccagaaatca cggcgtccaa tacgcccttt aaatgagaat aaccattgct gtatggtcat
    15721 gtaagttcct tgatggttgt cttttccagg attctaccct tttgacaagg gcgacagata
    15781 tcgttttaat cggagccagt cataacaaaa ggtactgtca atgaagccag ggtgtacgct
    15841 gttttttctc ttatgttctg cattaaccgt tacaacaacg gcgcatgcac aaacaccaga
    15901 tacggcaacg accgcacctt atctgctggc tggagcccct actttcgatc tctccatcag
    15961 ccagtttcga gaagacttta acagccagaa tcccagcctg ccactgaacg aatttcgtgc
    16021 catcgacagc agtcccgaca aagccaatct cactcgtgct gcaagtaaaa ttaacgagaa
    16081 cttgtatgct tctacagcgc tggagcgcgg taccttaaaa atcaaaagca ttcaaatgac
    16141 ctggctaccc atccaggggc cagagcaaaa agccgcgaaa gcgaaagctc aggaatatat
    16201 ggcagcggtg atccgcacac tcaccccatt aatgaccaaa acacaaagcc agaaaaaact
    16261 gcagtcgcta ctaacggcgg ggaaaaacaa acgttattac accgagacag aaggtgcact
    16321 gcgttatgtt gtcgcggaca acggcgaaaa ggggctgacc ttcgctgttg aaccgattaa
    16381 gctggcgcta tctgaatcgc ttgaaggttt gaataaatga caaaaagcaa agcctttgtg
    16441 ccgatgaatc tctatactgt ttcacagacc tgctgccctg cggggcggcc atcttccttt
    16501 attcgcttat aagcgtggag aattaaaatg cgacatcctt tagtgatggg taactggaaa
    16561 ctgaacggca gccgccacat ggttcacgag ctggtttcta acctgcgtaa agagctggca
    16621 ggtgttgctg gctgtgcggt tgcaatcgca ccaccggaaa tgtacatcga tatggcgaag
    16681 cgcgaagctg aaggcagcca catcatgctg ggtgcgcaaa acgtggacct gaacctgtcc
    16741 ggcgcattca ccggtgaaac ctctgctgct atgctgaaag acatcggcgc acagtacatc
    16801 atcatcggtc actctgaacg tcgtacttac cacaaagaat ctgacgaact gatcgcgaaa
    16861 aaattcgcgg tgctgaaaga gcagggcctg actccggttc tgtgcatcgg tgaaaccgaa
    16921 gctgaaaacg aagcgggcaa aactgaagaa gtttgcgcac gtcagatcga cgcggtactg
    16981 aaaactcagg gtgctgcggc attcgaaggt gcggttatcg cttacgaacc tgtatgggca
    17041 atcggtactg gcaaatctgc aactccggct caggcacagg ctgttcacaa attcattcgt
    17101 gaccacatcg ctaaagttga cgctaacatc gctgaacaag tgatcattca gtatggcggt
    17161 tctgtaaatg cttctaatgc tgcagaactg ttcgctcagc cggatatcga cggcgcgctg
    17221 gttggtggtg cttctctgaa agctgacgcc ttcgcagtaa tcgttaaagc tgcagaagcg
    17281 gctaaacagg cttaagtctg acaggtgccg gatttcatat ccggcactta ctttccttaa
    17341 ctcttcgcct taacgcaaaa tctcacactg atgatcctga atttcctcgg ctgaagcacg
    17401 gttaagtgtc agtagatttc gttgtgtcgc cagcaacaca aacgaattat cactctgccg
    17461 taccatcgcc agcccgtagc ttcccatatg ttcccgcgcc tcaggtactt cttctgccag
    17521 catcataaat gggctgcgtt gtaccagttc gctttccgtt acccgacgcg ccaggtattc
    17581 atgcccgcgc aaaccacctg gcagtggcaa ccagcggctg ctgatgttcg ccagattgtt
    17641 atccagctgt tcgcgcacat caggacgaat acaagagata tgaatatgaa aatggttttg
    17701 cgtacgcccg gtgcgggagt tgatcgccaa agaaaccgcg cgatcgggaa ccggctggcc
    17761 gtattttttg ctcataaaat cacgcgcctg ccaggccaac caaaagaagt tcggcgttga
    17821 aggatcggtc aacaaaggac tttcagtacc gttaatacga tacgttggca tcaacagata
    17881 ttgcagtggg ccattaagat cttttaaaac cacgtatccg gcattgggtt tgacttccgc
    17941 acatggcgaa ggattttgat tttgctgctg attgggcaaa cattcctcaa ggacaatctt
    18001 acgtaatgta tccgactctt caccggttaa tttccagtaa ccaataccgg cagccacaac
    18061 ggcgataact atcatcacca aaaaaagaag acccgctttt ttcatctttt tttccctgta
    18121 cctcaaagag atgcaagggt aacgcaaaat cgtgacaaat aaaaaacccg gtcgcgagaa
    18181 accaccgggt ttgataatta tcctggggaa atcagcgttt gctgatctga tcgaacgtac
    18241 cgccgttagc aaaatgctct ttttgcgctt tcgtccagcc gccgaactct tcatcaatgg
    18301 tgaataactt cagctttgga aacgcatttt cgtacttttt cgccacctca gcgtcgcgcg
    18361 gacggtagta gtttttcgcg gcaatttcct gaccttctgg cgagtagaga tatttcaggt
    18421 aggcttccgc cacctcttta gtaccttttt tctcgaccac tttatcgacc accgacacgg
    18481 ttggctctgc gaggatagac tcactcggcg tgacgatttc gaatttatct ttccccagtt
    18541 cattcgctgc cagcagagct tcgttttccc aggcaatcag tacatcgcca attccgcgct
    18601 cgacaaaagt gttagtggag ccgcgcgcgc cagaatccag aacttcgacg tttttataca
    18661 gtgcccgaac aaaatcctgt gcttttgcct gatcgttgtt gttgtgatgc agcgcgtagc
    18721 cccaggctgc caggtagttc cagcgcgcgc caccagagct tttcggatta ggcgtgatca
    18781 ctgaaacacc cggtttaatc agatcgttcc agtcatggat ctgcttcgga tttcccttac
    18841 gtaccaggaa aacaatggtg gaagtgtacg gtgcggagtt atccggcaga cgtttgatcc
    18901 actctttatc aatccgcccg cgttccgcaa tcgcgtccac gtcataggcc agagccagcg
    18961 tgacaacatc agcttcaata ccgttgatta ccgacgtcgc ttgtttacct gaaccaccgt
    19021 gtgactgacg aatcaccacg ttatcaccag tttgctgttt ccagtgggcg ctgaatgcct
    19081 tgttgtactg ttcgtacaat tcgcgcgttg gatcatatga aacgttaaga agctgaatat
    19141 cctttgccat aacgctggtt gccgccagca aaaatgttaa ccctacgccc cacttgttca
    19201 tcgcccgact ctcttatgtt gtgttgtgat gagcaaagcg tgccagaagg ttaaccaaac
    19261 attaaagaat aaaaaaagat tggctataac ttgtgggtat atgttgaggg attaaaaagg
    19321 cggaagaaat ccgcctcata ttgctgacaa agtgcgcttt gtccatgccg gatgcggcgc
    19381 gaacgcctta tccggcctac aaaagtttgc aaattcaata aattgcagaa ttcatgtagg
    19441 cctgataagc gaagcgcatc aggcattttt gcttctgtca tcggtttcag gctaaaggaa
    19501 tctgcctttt tccgaaatca ttaatacagt tttttcgcgc agtccagcca gtcacctttg
    19561 aacggacgct tcatgttttc gatagcgtcg atgatgtcgt ggtgaaccag ctgttcgttc
    19621 tggataccta cacaacgacc gccgtaacct gccagcagca gatcgatagc gtaagcgccc
    19681 atacgggaag ccagaatacg gtcgtaaggc accggagaac caccgcgctg gatgtggccc
    19741 agcacagttg cgcgggtttc acgaccggtt tctttctcga tgaaatgcgc cagttcgtca
    19801 acatcacaca tatgttcggt aatcgccacg atcgcgtgtt ttttaccttt cgcgataccc
    19861 gctttgattt cgtttaccag gtcttcacgg ctgaattcaa cttccggaac cacaacgaat
    19921 tcacagccac cggcaatggc cgcagccagg gtcagatcgc cacaataacg gcccatcact
    19981 tccaccacgg aaatacgctg gtgagaagaa gaggtgtcac gcagacggtc gatcgcttct
    20041 acaacggtgc tcagcgcagt gaagaaaccg atagtgtagt cagtgccttt gatgtcgttg
    20101 tcgatagtgc ccggcagacc gatgcacggg aagcccattt cggtcagacg cattgcaccc
    20161 atgtaggaac cgtcaccgcc gataaccacc agcgcgtcga taccacgttt tttcaggttt
    20221 tcgatagcca cggcgcggat gttctcgtcg cggaattccg ggaagcgcgc agaaccgagg
    20281 aacgtaccgc cacggttgat catgtcagaa acgctgtaac ggtctagctg taccatacgg
    20341 tcttcataca gacccagata gccgtcataa atgcccatta cttccagacc ttctgtcagc
    20401 gcagaacgaa caaccccgcg aattgcggcg ttcatgcctg gcgcatcacc gccgcttgtc
    20461 aacacaccga ttttcttaat catgactacc tctgaacttt ggaatgcaaa ataaaatctg
    20521 ttgccggaag tcttcttgca catcgaagtg atccaacgaa tgtgcaaata gtataacaat
    20581 cacttcctgc tgaattgatt caggtcaggc caaatggcgg tattttatac acaaaatgcg
    20641 ggtctggctc tcttttatac tgattatgaa agcatagacc gtttaccctc cctgggtacg
    20701 acggaacagg ggtcctgatg gataattaca tccgatcccg gaaaacgccg taaaatagcc
    20761 tgctctacct gatccgccac catatgtgcc tgaaccaaag gcagagagtc ttccatttcc
    20821 aaatgaatct gaataaagcg ggtcggccct gactgccgcg tgcgaagatc gtgagcgccg
    20881 ctaacacccg gccaggaagt cacgatatca ataatttctt gccgttcctc atcaggcaat
    20941 gcgcgatcca gtaatgactg taccgcctca tatcccatgc gtaacgcgct atataaaata
    21001 tagatgccga ttcccaatgc aaacagagca tcggcgcgat gccagccgta ccaggacaac
    21061 cccagcgcca gcagaattgc gccgttcatc ataacatcag actggtaatg tagcatatca
    21121 gcccgcaccg cctggctttg cgtccgccgc accacccaac gctgaaacga gacaaggata
    21181 atcgtacaaa ttagcgccac aattgtcacg ataaccccga cgcctggatc tgtcatcggt
    21241 gttggagata tcagatgttg aatacccgtc aaaaacagga atagtgccga accggagata
    21301 aacatacttt gcgccagcgc cgcgagggac tctgctttac cgtgaccaaa cgagtgatta
    21361 tcgtcggcag gttgcaggga atatcgcacc accagtaaat tcgtcaacga cgcgccgata
    21421 tccaccagcg aatccaccag cgcggcgaga atactcaccg acccggtata ccaccatgca
    21481 aaaattttaa tcagcaatag caacgaagcc atcgccgtcg cagcaatcgc cgcccgactg
    21541 accagccgtc cataagattg attcataaat actcccgcta tcaactgacg ctagtataac
    21601 ggaagcaaat catctgcaat gcattaagca gcaggcaaat tgaggataaa aaaaaccccc
    21661 acatcatgtg ggggaagaca gggatggtgt ctatggcaag gaaaacaggg tttactactg
    21721 ggaacgtgag ttgctactac tcaatagctt caacgatgaa cttttttgcc attgcgtcac
    21781 gtcacgcaac tgctccattc gttgttgatg tttctcgttt aaaaccgctt gctgctccgg
    21841 cgttaacagg cgatacattt ggttgcggac ttttgccatc tcaacctgac gagcaatttg
    21901 ctcattcgcc attttttctg cctgtgcgcg cacagcgttt tcatcaaaat tttctgcggt
    21961 gacaaggcga tgcattgtct ccagttcgct aacattaaca ggaggctgtt cgtgccgggc
    22021 ctgttgcata agatctcgca tctgctgacg ctgatgttcg gttaaactta tgccgtcgaa
    22081 catatggctc tgcgtactgc gctgcgtaag ttcttcaccc ggatgccagt tatcgcctga
    22141 accgacttca gcagcgtggc ttaatgaact gactgccagc gttgaggcca tgacggcagc
    22201 ggtaactatg cgcatcattt gctcccaaaa tctttctgtc gcgattcaac gatagagagt
    22261 ttacgattca ggctgcaaac atgcgtcagg gggtgtaaaa caacgtaaag tcatggatta
    22321 gcgacgtctg atgacgtaat ttctgcctcg gaggtattta aacaatgaat aaaatcctgt
    22381 tagttgatga tgaccgagag ctgacttccc tattaaagga gctgctcgag atggaaggct
    22441 tcaacgtgat tgttgcccac gatggggaac aggcgcttga tcttctggac gacagcattg
    22501 atttactttt gcttgacgta atgatgccga agaaaaatgg tatcgacaca ttaaaagcac
    22561 ttcgccagac acaccagacg cctgtcatta tgttgacggc gcgcggcagt gaacttgatc
    22621 gcgttctcgg ccttgagctg ggcgcagatg actatctccc gaaaccgttt aatgatcgtg
    22681 agctggtggc acgtattcgc gcgatcctgc gccgttcgca ctggagcgag caacagcaaa
    22741 acaacgacaa cggttcaccg acactggaag ttgatgcctt agtgctgaat ccaggccgtc
    22801 aggaagccag cttcgacggg caaacgctgg agttaaccgg tactgagttt accctgctct
    22861 atttgctggc acagcatctg ggtcaggtgg tttcccgtga acatttaagc caggaagtgt
    22921 tgggcaaacg cctgacgcct ttcgaccgcg ctattgatat gcacatttcc aacctgcgtc
    22981 gtaaactgcc ggatcgtaaa gatggtcacc cgtggtttaa aaccttgcgt ggtcgcggct
    23041 atctgatggt ttctgcttca tgataggcag cttaaccgcg cgcatcttcg ccatcttctg
    23101 gctgacgctg gcgctggtgt tgatgttggt tttgatgtta cccaagctcg attcacgcca
    23161 gatgaccgag cttctggata gcgaacagcg tcaggggctg atgattgagc agcatgtcga
    23221 agcggagctg gcgaacgatc cgcccaacga tttaatgtgg tggcggcgtc tgttccgggc
    23281 gattgataag tgggcaccgc caggacagcg tttgttattg gtgaccaccg aaggccgcgt
    23341 gatcggcgct gaacgcagcg aaatgcagat cattcgtaac tttattggtc aggccgataa
    23401 cgccgatcat ccgcagaaga aaaagtatgg ccgcgtggaa ctggtcggtc cgttctccgt
    23461 gcgtgatggc gaagataatt accaacttta tctgattcgt ccggccagca gttctcaatc
    23521 cgatttcatt aacttactgt ttgaccgccc gctattactg ctgattgtca ccatgctggt
    23581 cagtacgccg ctgctgttgt ggttggcctg gagtctggca aaaccggcgc gtaagctgaa
    23641 aaatgctgcc gatgaagttg cccagggaaa cttacgccag cacccggaac tggaagcggg
    23701 gccacaggaa ttccttgccg caggtgccag ttttaaccag atggtcaccg cgctggagcg
    23761 catgatgacc tctcagcagc gtctgctttc tgatatctct cacgagctgc gcaccccgct
    23821 gacgcgtctg caactgggta cggcgttact gcgccgtcgt agtggtgaaa gcaaggaact
    23881 ggagcgtatt gaaaccgaag cgcaacgtct ggacagcatg atcaacgatc tgttggtgat
    23941 gtcacgtaat cagcagaaaa acgcgctggt aagcgagaca ttgaaagcca accagttgtg
    24001 gagtgaagtg ctggataacg cggcgttcga agccgagcaa atgggcaagt cgttgacagt
    24061 taacttcccg cctgggccgt ggccgctgta cggcaatccg aacgccctgg aaagtgcgct
    24121 ggaaaacatt gttcgtaatg ctctgcgtta ttcccatacg aagattgaag tgggctttgc
    24181 ggtagataaa gacggtatca ccattacggt ggacgacgat ggtcctggcg ttagcccgga
    24241 agatcgcgaa cagattttcc gtccgttcta tcgtaccgat gaagcgcgcg atcgtgaatc
    24301 tggcggtaca ggattggggc tggcgattgt tgaaaccgcc attcagcagc atcgtggctg
    24361 ggtgaaagca gaagacagcc cgctgggcgg tttacggctg gtgatttggt tgccgctgta
    24421 taagcggagt taaactccgc atttgtaggc aggataaggc gtttacgccg catacggcat
    24481 ttgagcagga tgcctgatgc gacgctgata gcgtcttatc aggcctacac tccgagggtt
    24541 attttcccca caaccgccgg gaaaaatctt cgatcttgcc gctcagtcgg cgcttttgca
    24601 tcgtccgcgt ccagcttttc gataacccgg cggcggagag taaacggtga tactgatcgt
    24661 catcaaacgg catatgccat gcgatggcgg cagcctcctg cacggtcaca tcgctgacac
    24721 gggaaaccaa ctccagcggc gcgtccgcag acactttccc tggcgctatc acgctataca
    24781 accatcccac tttacccgtg ttttgcatca actgcgctat atcgctgata tcaaaatggt
    24841 aattgagttt atagcaaggc gagcgcggct ggctgacctg aattaatgcc tctccccagc
    24901 ggaaaatatc gcccatataa acattacttt ccgtcagacc gtcggttgag agattttcgc
    24961 caaacgcagg cgccacaaac aactccgcct gttccggaaa ttcccgcgcc cagtagagat
    25021 aatgctcacg aggataatga cacagcgctc tgtctggtcc gccgtgaacc tttttctccg
    25081 cctgctcgtc accttccagc cccagctccg tcagcatcaa ctcaccatca acctggattt
    25141 tggcaattgc gctgggtttg ccttcgggat aagcctgaat cttgcctgtg tatacatcaa
    25201 ccggatatcg catctgttcc ctctccacga gaaatttccc cattagacca tatttatttt
    25261 atgtcaggcg tgctgttttc gcggtaagta tccaggcagg aactcaatag caaaccggac
    25321 gaacgttagt tcatccggcg gtggggatta aggaagcagg tttatttcac cgtacctaaa
    25381 atttcgattt tcgccgctct ggctttgact ttacgtgacc agatagaggt aagaattggc
    25441 accagaatcg aagtcacaat gactgccgtc gctaccagcg aagttgctgc cggagccatc
    25501 ggtttaaacg caggcaccat ttctgcaatc agcacaggtg tcgctaccgc ggcccctgcg
    25561 gaactggaag cggcaattcc ggcagtgcca tcgccaccgc caatcaattt atcggcgata
    25621 atcaacggaa taccggtcac gataattact gccacaccca acaggatccc cagcaaacct
    25681 gtctgggcaa ttacagtcaa atcaatggta ttgcctagtg cgaaggcaaa gaatggaatc
    25741 agcgtctgca ccgctttgct gaaaaattct cgtaattctg ggtcaaggtt cccaagggca
    25801 aagcccacca ggaacggtaa tactgcgccg acgaaaacat gcggttcaaa cgaggcaatc
    25861 ccggcagtgc ccagaataat catcgtcatg agcggcccgg actccaacga catcaacaca
    25921 aatgccccag cttcttcttt tgtgccgtac tgctgcatga tggaagcgta aagtccgccg
    25981 ttggtcatat ccatcgccgc caccagcgcc agcgttgaaa gtccggcaaa gaatccaact
    26041 tcaacaccat gttccggaat aatgcgcgag gcaattgccg caaccaccca cgcgacggca
    26101 attttagtta ccaccagcgt accggattta cgcagtaccg ttcccgtcgc gcttaatttt
    26161 attgacgccc ccatgcaaaa aaaccacacc gccagaatgg gcaccgtacc ggtaatcatc
    26221 ccgttggtga atgatccaaa atatttcccc gcccccggcg agaaggtgtg gcacagtgcg
    26281 ccaaggaata gcgggacgag catcatcccc cccgggattt tctcaatcga gcgttttatc
    26341 tgcatttcca tcacctgtcg gcatattttt aagggctgat gcgtaattcc tcaatggagc
    26401 atcagtgttg atggaaacga agatagacat tcatttaatc aataaaagtg atcaaaacca
    26461 catcaattga aacgctgttt tatttttatc ggatcattgt ttttattttg aaattgatca
    26521 caaaaaaacc accgccattg gcgatggttc attttttaag ctgatatgcg gcgtaaacgc
    26581 ctcattgcag cagacggcaa atgattattt tttcgccgcg aaacgtgccg ctgcttcgtc
    26641 ccagttcacc acgttccaga actctttgat gtagtccgga cggcggttct ggaatttcag
    26701 gtagtaagca tgttcccaca catccaggcc cagaatcggg aagccggaag cgccagaaat
    26761 agcttcaccc atcagcgggg agtcctggtt agcagtagaa accaccgcca gtttatcgcc
    26821 tttcagcacc agccatgccc agccggaacc aaagcgggaa gctgccgctt tttcaaattc
    26881 tgctttgaag ttatctacgg aaccgaaatc acgttcgata gccgctttca ggtcaccctg
    26941 cagggtggtg ccttttttca gacctttcca gaacaggctg tggttagcgt gaccgccagc
    27001 gttgttgcgc agtacggttt tcttgtctgc tggcagctgg tccagtttgg tgatcaactc
    27061 ttcaaccggc aggttggcaa attctggcag gctttccaac gccgcgttgg cgttgttgac
    27121 gtaggtctga tggtgtttgg tgtggtggat ttccatggtc tgcttatcga agtgcggttc
    27181 cagggcatcg taagcatacg gcagggatgg cagggtatag ctcatattca tctccagtat
    27241 tgtcgggcgg ccgattgtta atgccgcgta agcagttggt tcattatagt taattaaatg
    27301 atattgaaaa tgattatcaa tgccgtactt ttcgtaaggg tatggttttg caggaaaatg
    27361 cccgagatgt gaagcaaatc acccacttaa tgccgtgatt gccagtaaat cgacaacggc
    27421 ggcaacaggc gaaaggttaa tcgacagcac gatttttaca ctcatctcgt cggagatgtg
    27481 acgcgacgaa aaatgatgag gataagaaga tgagtaacgc gattacgatg gggatatttt
    27541 ggcatttgat cggcgcggcc agtgcagcct gtttttacgc tccgttcaaa aaagtaaaaa
    27601 aatggtcatg ggaaaccatg tggtcagtcg gtgggattgt ttcgtggatt attctgccgt
    27661 gggccatcag cgccctgtta ctaccgaatt tctgggcgta ttacagctcg tttagtctct
    27721 ctacgctgct gcctgttttt ctgttcggcg ctatgtgggg gatcggtaat atcaactacg
    27781 gcctgaccat gcgttatctc ggcatgtcga tgggaattgg catcgccatt ggcattacgt
    27841 tgattgtcgg tacgctgatg acgccaatta tcaacggcaa tttcgatgtg ttgattaaca
    27901 ccgaaggcgg acgcatgacg ttgctcggcg ttctggtggc gctgattggc gtagggattg
    27961 taactcgagc cgggcagttg aaagagcgca agatgggtat taaagccgaa gagttcaatc
    28021 tgaaaaaagg gctggtgctg gcggtgatgt gcggcatttt ctctgccggg atgtcctttg
    28081 cgatgaacgc cgcaaaaccg atgcatgaag ccgctgccgc acttggcgtc gatccactgt
    28141 atgtcgctct gccaagctat gttgtcatca tgggcggcgg cgcgatcatc aacctcggtt
    28201 tctgttttat tcgtctggca aaagtaaagg atttgtcgct aaaagccgac ttctcactgg
    28261 caaaaccgct gatcattcac aatctgttac tctcagcact gggcggattg atgtggtatc
    28321 tgcaattctt tttctatgcc tggggccacg cccgcattcc ggcgcagtat gactacatca
    28381 gttggatgct gcatatgagc ttctatgtat tgtgcggcgg tatcgtcggg ctggtgctga
    28441 aagagtggaa caatgcggga cgccgtccgg taacggtgtt gagcctcggt tgtgtggtga
    28501 ttattgtcgc cgctaacatc gtcggcatcg gcatggcgaa ttaatctttc tgcgaattga
    28561 gatgacgcca ctggctgggc gtcatcccgg tttcccgggt aaacaccacc gaaaaatagt
    28621 tactatcttc aaagccacat tcggtcgaaa tatcactgat taacaggcgg ctatgctgga
    28681 gaagatattg cgcatgacac actctgacct gccgcagata ttgattgatg gtcattccag
    28741 tctgctggcg aaattgctga cgcaaaacgc gctcactgca cgatgcctca tcacaaaatt
    28801 tatccagcgc aaagggactt ttcaggctag ccgccaaccg ggtaatcagc ttatccagca
    28861 acgtttcgct ggatgttggc ggcaacgaat cactggtgta acgatggcga ttcagcaaca
    28921 tcaccaactg cccgaacagc aactctgcca tttcgttagc aaacggcaca tgctgactac
    28981 tttcatgctc aagctgaccg ataatctgcc gcgcctgtgc catccccatg ctacctaagc
    29041 gccagtgtgg ttgccctgcg ctgacgctaa atcccggaat cgccccctgc cagtcaagat
    29101 tcagcttcag ccgctccggg caataaataa tattctgcaa aaccagatcg ttaacggaag
    29161 cgtaggagtg tttatcgtca gcatgaatgt aaaagagatc gccacgggta atgcgataag
    29221 ggcgatcgtt gagtacatgc aggccattac cgcgccagac aatcaccagc tcacaaaaat
    29281 catgtgtatg ttcagcaaag acatcttgcg gatatcggtc agccacagcg actgcctgct
    29341 ggtcgctggc aaaaaaatca tctttgagaa gttttaattg atgcgccacc gtggctacct
    29401 cggccagaga acgaagttga ttattcgcaa tatggcgtac aaatacgttg agaagattcg
    29461 cgttattgca gaaaaccatc ccgtccctga cgaatatcac gcggtgacca gttaaactct
    29521 cggcgaaaaa gcgtcgaaaa gtggttactg tcgctgaatc cgcagcgata ggcgatgtca
    29581 gtaacgctgg cctcgctgtg gcgtagcaga tgtcgggctt tcatcagtcg caggcggttc
    29641 aggtatcgct gaggcgtcag tcccgtttgc tgcttaagct gccgatgtag cgtacgcagt
    29701 gaaagagaaa attgatccgc cacggcatcc caattcacct catcggcaaa atggtcctcc
    29761 agccaggcca gaagcaagtt gagacgtgat gcgctgtttt ccaggttctc ctgcaaactg
    29821 cttttacgca gcaagagcag taattgcata aacaagatct cgcgactggc ggtcgagggt
    29881 aaatcatttt ccccttcctg ctgttccatc tgtgcaacca gctgtcgcac ctgctgcaat
    29941 acgctgtggt taacgcgcca gtgggaagga tactgtccgt cctgctcttg tggcagcaac
    30001 tgattcagcc cggcgagaaa ctgaaatcga tccggcgagc gatacagcac attagtcaga
    30061 cacagattat cggtatgttc atacagatgc cgatcatggt cgcgcacaaa acagaccgtg
    30121 ccaccggtga tggtataggg ctgcccatta aacacatgaa tacccgtgcc atgttcgaca
    30181 atcacaattt catgaaaatc atgatgatgt tcaggaaaat ccgcctgcgg gagccggggt
    30241 tctatcgcca cggacgcgtt accagacgga aaaaaatcca cactatgtaa tacggtcata
    30301 ctggcctcct gatgtcgtca acacggcgaa atagtaatca cgaggtcagg ttcttacctt
    30361 aaattttcga cggaaaacca cgtaaaaaac gtcgattttt caagatacag cgtgaatttt
    30421 caggaaatgc ggtgagcatc acatcaccac aattcagcaa attgtgaaca tcatcacgtt
    30481 catctttccc tggttgccaa tggcccattt tcctgtcagt aacgagaagg tcgcgaattc
    30541 aggcgctttt tagactggtc gtaatgaaat tcagcaggat cacattatga cctttcgcaa
    30601 ttgtgtcgcc gtcgatctcg gcgcatccag tgggcgcgtg atgctggcgc gttacgagcg
    30661 tgaatgccgc agcctgacgc tgcgcgaaat ccatcgtttt aacaatgggc tgcatagtca
    30721 gaacggctat gtcacctggg atgtggatag ccttgaaagt gccattcgcc ttggattaaa
    30781 caaggtgtgc gaggaaggga ttcgtatcga tagcattggg attgatacct ggggcgtgga
    30841 ctttgtgctg ctcgaccaac agggtcagcg tgtgggcctg cccgttgctt atcgcgatag
    30901 ccgcaccaat ggcctaatgg cgcaggcaca acaacaactc ggcaaacgcg atatttatca
    30961 acgtagcggc atccagtttc tgcccttcaa tacgctttat cagttgcgtg cgctgacgga
    31021 gcaacaacct gaacttattc cacacattgc tcacgctctg ctaatgccgg attacttcag
    31081 ttatcgcctg accggcaaga tgaactggga gtacaccaac gccacgacca cgcaactggt
    31141 caatatcaat agcgacgact gggacgagtc gctactggcg tggagcggtg ccaacaaagc
    31201 ctggtttggt cgcccgacgc atccgggtaa tgtcataggt cactggattt gcccgcaggg
    31261 taatgagatt ccggtggtcg ccgttgccag ccatgatacc gccagcgcgg ttatcgcctc
    31321 gccgttaaac ggttcccgcg ccgcttatct ctcctctggc acctggtcat tgatgggttt
    31381 tgaaagcaaa acgccattta ccaatgacac ggcgctggca gccaacatca ccaatgaagg
    31441 cggggcggaa ggtcgctatc gggtgctgaa aaatattatg ggcttatggc tgcttcagcg
    31501 agtgcttcag gagcggcaaa tcaacgatct cccggcgctt atcgccgcga cacaggtact
    31561 tccggcctgc cgcttcatca tcaatcccaa tgacgatcgc tttattaatc ctgaagcgat
    31621 gtgcagcgaa attcaggctg cgtgtcggga aacggcgcaa ccgatcccgg aaagtgatgc
    31681 tgaactggcg cgctgtattt tcgacagtct ggcgttgctg tatgccgatg tgttgcatga
    31741 gctggcgcag ctacgcggtg aagatttctc gcaactgcat attgtcggcg gcggctgcca
    31801 gaacacgcta ctcaaccagc tatgtgccga tgcctgcggt attcgggtga tcgccgggcc
    31861 tgttgaagcc tcgacgctcg gcaatatcgg catccagtta atgacgctgg atgaactcaa
    31921 caatgtggat gatttccgtc aggtcgtcag cactaccgcg aatctgacca cctttacccc
    31981 taatcctgac agtgaaattg cccactatgt ggcgcagatt cactctacac gacagacaaa
    32041 ggagctttgc gcatgaccac tcaactggaa caggcctggg agctggcgaa acagcgtttc
    32101 gcggcggtgg ggattgatgt cgaggaggcg ctgcgccaac ttgatcgttt acccgtttca
    32161 atacactgct ggcagggcga tgatgtttcc ggttttgaaa acccggaagg ttcgctgacc
    32221 ggtggtattc aggctactgg taattatccg ggcaaagcgc gtaatgccag tgagctacgt
    32281 gccgatctgg aacaggctat gcggctgatt ccggggccga aacggcttaa tttacatgcc
    32341 atctatctgg aatcagacac gccagtctcg cgcgaccaaa tcaaaccaga gcacttcaaa
    32401 aactgggttg aatgggcgaa agccaatcag ctcggtctgg attttaaccc ctcctgcttt
    32461 tcgcatccgc taagcgccga tggctttacg ctttcccatg ccgacgacag cattcgccag
    32521 ttctggattg atcactgcaa agccagccgt cgcgtttcgg cctattttgg cgagcaactc
    32581 ggcacaccat cggtgatgaa catctggatc ccggatggta tgaaagatat caccgttgac
    32641 cgtctcgccc cgcgtcagcg tctgctggca gcactggatg aggtgatcag cgagaagcta
    32701 aaccctgcgc accatatcga cgccgttgag agcaaattgt ttggcattgg cgcggagagc
    32761 tacacggttg gttccaatga gttttacctg gggtatgcca ccagccgcca gacggcgctg
    32821 tgcctggacg ccgggcattt ccacccgact gaagtgattt ccgacaagat ttccgccgcc
    32881 atgctgtatg tgccgcagtt gctgctgcac gtcagccgtc cggttcgctg ggacagcgat
    32941 cacgtggtgc tgctggatga tgaaacccag gcgattgcca gtgagattgt tcgtcacgat
    33001 ctgtttgacc gggtgcatat cggccttgac ttcttcgatg cctctatcaa ccgcattgcc
    33061 gcgtgggtca ttggtacacg caatatgaaa aaagccctgc tgcgtgcgtt gctggaacct
    33121 accgctgagc tgcgcaagct ggaagcggcg ggcgattaca ctgcgcgtct ggcactgctg
    33181 gaagagcaga aatcgttgcc gtggcaggca gtatgggaaa tgtattgcca acgtcatgat
    33241 acgccagcag gtagcgaatg gctggagagc gtgcgggcat atgagaaagc gattttgagc
    33301 cagcgtgggt aaacactgcc ggatgcggcg cgagcgcctt atccggccta caggtcggca
    33361 a
//
LOCUS       DAPGOF010000037        33125 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.037, whole genome shotgun sequence.
ACCESSION   DAPGOF010000037 DAPGOF010000000
VERSION     DAPGOF010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33125
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.037"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            428..649
                     /locus_tag="RHV13_001505"
     CDS             428..649
                     /locus_tag="RHV13_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HDV1241058.1"
                     /translation="MVNANPCARQEFIWRFYSCKKHHYHFVIAATEDEARSQLPDGPC
                     IFTARFSTNSRNPLSYWSLPFSADVQGGL"
     gene            646..1689
                     /locus_tag="RHV13_001506"
     CDS             646..1689
                     /locus_tag="RHV13_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015974252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HDV1241059.1"
                     /translation="MKKPLVTRNDIAEAIALHTACMPTREIPGAIANYFMITRRFYTR
                     TDKAVINKLLIAEIRDYLIEQGRLRYATVAAEMRKEAHRMTGNNLNVEKTAPVTSATP
                     APAVNIIPNTGDTIDSLTLLKMVNEARKLCGEPEVRNNKFIEKILDELEGEDGYTKSA
                     TVPPGGGTPMVVITMTYKQALRVAARESKAVRRSLIDKLEELQQANSPTPSIPQTLPE
                     ALRLAAELAEQKMQLEQQLVAAAPKVDFADRVSVANGILIGNFAKVVGLKQNALFSWL
                     RQNGILMAFGARKNVPRQQYINAGYFTVKEVVLDDENGYQIRLTPQLTGKGQQWLTRK
                     LLDAGLLKPVAIG"
     gene            1854..2654
                     /locus_tag="RHV13_001507"
     CDS             1854..2654
                     /locus_tag="RHV13_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001187871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HDV1241060.1"
                     /translation="MQQTFNADMNISNLHQNVDPSTTLPVICGVEITTDRAGRYNLNA
                     LHRASGLGAHKAPAQWLRTLSAKQLIEELEKETMQNCIVSFEGRGGGTFAHELLAVEY
                     AGWISPAFRLKVNQTFIDYRAGRLQPAIPKSLPEALRLAADLAEQKQRLEQKMLMDAP
                     KVEFAERVATASGVLIGNYAKVLGLGQNYLFTWLRDNGILIATGERRNVPKQEYISRG
                     YFTLKETVIDTSNGSRISFTTRITGKGQQWLMKRLLDAGVLVPVAATR"
     gene            2684..3529
                     /locus_tag="RHV13_001508"
     CDS             2684..3529
                     /locus_tag="RHV13_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein repL"
                     /protein_id="HDV1241061.1"
                     /translation="MLAKVTFLSCITMSDFTFSGYELACFVTHSGLSRSAGHILSQCA
                     NLAATTSEYFIHKPHRLIAAETGYSQSTVVRAFREAVNKGILSVEIVIGDHRERRANL
                     YRFTPSFLAFAQQAKNALIESKLKISSAATKVKAVLAKTLALFNFLSTPPCQNDTPSP
                     CQDDVAIKNKKSQVKKTKRSVSGGAGTTRLKKLTSWIAEAKAKADNLRLSKKRAQKHE
                     FKQKVEAAARKYAYLKNKRSPDIGGVSNFDNLPHCMTVNEALNAVLAKNKDNEQWGIP
                     AGFRG"
     gene            3580..3825
                     /locus_tag="RHV13_001509"
     CDS             3580..3825
                     /locus_tag="RHV13_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002433487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1241062.1"
                     /translation="MQSDFLQLAIAFAGYVCIGFCVYMFSRKMLVDIDRKEQAEEILV
                     WIFFGAVWPLGIMFAATFLLMWIFTLPGDFYRKKARH"
     gene            4007..4162
                     /gene="hokD"
                     /locus_tag="RHV13_001510"
     CDS             4007..4162
                     /gene="hokD"
                     /locus_tag="RHV13_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HDV1241063.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVTVF
                     TAYESER"
     gene            complement(4279..4788)
                     /locus_tag="RHV13_001511"
     CDS             complement(4279..4788)
                     /locus_tag="RHV13_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HDV1241064.1"
                     /translation="MGHNNTKGNRKFIKGRYTANAAKGERLVSSEFQLTFAGHEDISV
                     LVRTSQIPEMTREDVEDYGPNGVKFNQHGPIRNSGEIQVQCVETIEGDILQFIKDRIA
                     AKDYVDITMAATPESKSSGVNAVTKAATTIEMLDCKIYSDAIDFSTEDVTAAVRPSLR
                     IVYNWIEWD"
     gene            complement(4800..5381)
                     /locus_tag="RHV13_001512"
     CDS             complement(4800..5381)
                     /locus_tag="RHV13_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000035303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1241065.1"
                     /translation="MAPIPYGVYSQADGVSPFLKVTLTNSQYQVTGYISQGAAMNMAQ
                     NWEAPFTGMSMGSVAGAFSGFAQVGTETTSVARWNSLMVWEGGTPPTFTLPVTFIALF
                     DPFTEVSGAIAALSAMISPELKDASIGGRIPERVTLNIGRRINIIDVAIQDISFDLDA
                     PRDSNGHFLKNTVNLQLTGSSIYNSSDIVRAFQ"
     gene            complement(5417..6232)
                     /locus_tag="RHV13_001513"
     CDS             complement(5417..6232)
                     /locus_tag="RHV13_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1241066.1"
                     /translation="MSKNFFQSGAFLGNGLSRFALNSDPVQLMESARASAEPPTDPVI
                     NNNPEPAAQTNDNVPSAPAPEQILEGKDGKEWTVEQAHQMILEAANRSAMQNALSDAA
                     DAVFAWADSGDLTFDSLDGFVQAIAGISDDDDSEVTEEQDDAYNEAWANVADFLAACG
                     VDDDLIEALADDEDDDAAADVGASIAGLDSDDRDELEAAFVVAGTSDEMLTEAFKKVV
                     RNGEIKLIRKRLRKKRLTAAQKSALKKARRKAQTGAAKLARKKSMKLRRKRLG"
     gene            complement(6242..7831)
                     /locus_tag="RHV13_001514"
     CDS             complement(6242..7831)
                     /locus_tag="RHV13_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1241067.1"
                     /translation="MSQYSIQQSLGNASGVAVSPINADATLSTGVALNSSLWAGIGVF
                     ARGKPFTVLAVTESNYEDVLGEPLKPSSGSQFEPIRHVYEAIQQTSGYVVRAVPDDAK
                     FPIIMFDESGEPAYSALPYGSEIELDSGEAFAIYVDDGDPCISPTRELTIETATADSA
                     GNERFLLKLTQTTSLGVVTTLETHTVSLAEEAKDDMGRLCYLPTALEARSKYLRAVVN
                     EELISTAKVTNKKSLAFTGGTNGDQSKISTAAYLRAVKVLNNAPYMYTAVLGLGCYDN
                     AAITALGKICADRLIDGFFDVKPTLTYTEAISAVEDTGLLGTDYVSCAVYHYPFSCKD
                     KWTQSRVVFGLSGVAYAAKARGVKKNSDVGGWHYSPAGEERAVIARASIQPLYPEDTP
                     DEEAMVKGRLNKVSVGTSGQMIIDDALTCCTQDNYLHFQHVPSLMNAISRFFVQLARQ
                     MKHSPDGITAAGLTKGMTKLLDRFVASGALVAPRDPDADGTEPYVLKVTQAEFDKWEV
                     VWACCPTGVARRIQGVPLLIK"
     gene            complement(7892..9598)
                     /locus_tag="RHV13_001515"
     CDS             complement(7892..9598)
                     /locus_tag="RHV13_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1241068.1"
                     /translation="MSNLREYQNRIADIAKRSKAVLGWASTAQFGTDNQFIKDDAARA
                     ASILEAARKDPVFAGISDNATAQIATAWASALADYAAAHKSMPRPEILASCHQTLENC
                     LIESTRNSMDATNKAMLESVAAEMMSVSDGVMRLPLFLAMILPVQLGAATADACTFIP
                     VTRDQSDIYEVFNVAGSSFGSYAAGDVLDMQSVGVYSQLRRRYVLVASSDGTSKTATF
                     KMEDFEGQNVPIRKGRTNIYVNRIKSVVDNGSGSLLHSFTNAAGEQITVTCSLNYNIG
                     QIALSFSKAPDKGTEIAIETEINIEAAPELIPLINHEMKKYTLFPSQFVIAAEHTVQA
                     AYEAQREFGLDLGSLQFRTLKEYLSHEQDMLRLRIMIWRTLATDTFDIALPVNQSFDV
                     WATIIRGKFQTVYRDIIERVKSSGAMGMFAGADAASFFKQLPKDFFQPAEDYIQTPYV
                     HYIGTLFGNVKVYEVPAGICKNLTTENIQFSSMDVLCYVRDENPGKAGFVTGDAVPAI
                     PFQHPTTPALVNRTTLWGSAINDMHPRNGADYFTRVTLTMAKKGGLNFISGDTIDAGD
                     SE"
     gene            complement(9824..10825)
                     /locus_tag="RHV13_001516"
     CDS             complement(9824..10825)
                     /locus_tag="RHV13_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDV1241069.1"
                     /translation="MSKKNRPTIGRTLNPSILSGFDSSSASGDRVEQVFKLSTGRQAT
                     FIEEVIPPNQVESDTFVDQHNNGRDQASLTPKSLKSIRSTIKHQQFYPAIGVRRATGK
                     IEILDGSRRRASAILENVGLRVLVTDQEISVQEAQNLAKDVQTALQHSIREIGLRLMR
                     MKNDGMSQKDIAAKEGLSQAKVTRALQAASAPEELVALFPVQSELTFSDYKTLCAVGD
                     EMGNKNLEFDQLIQNISPEINDILSIEEMAEDEVKNKILRLITKEASLLTDKGSKDKS
                     VVTELWKFEDKDRFARKRVKGRAFSYEFNRLSKELQEELDRMIGHILRKSLDKKPKP"
     gene            complement(10842..12038)
                     /locus_tag="RHV13_001517"
     CDS             complement(10842..12038)
                     /locus_tag="RHV13_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDV1241070.1"
                     /translation="MSDSSQLHKVAQRANRMLNVLTEQVQLQKDELHANEFYQVYAKA
                     ALAKLPLLTRANVDYAVSEMEEKGYVFDKRPAGSSMKYAMSIQNIIDIYEHRGVPKYR
                     DRYSEAYVIFISNLKGGVSKTVSTVSLAHAMRAHPHLLMEDLRILVIDLDPQSSATMF
                     LSHKHSIGIVNATSAQAMLQNVSREELLEEFIVPSVVPGVDVMPASIDDAFIASDWRE
                     LCNEHLPGQNIHAVLKENVIDKLKSDYDFILVDSGPHLDAFLKNALASANILFTPLPP
                     ATVDFHSSLKYVARLPELVKLISDEGCECQLATNIGFMSKLSNKADHKYCHSLAKEVF
                     GGDMLDVVLPRLDGFERCGESFDTVISANPATYVGSADALKNARIAAEDFAKAVFDRI
                     EFIRSN"
     gene            complement(12597..13457)
                     /gene="repA"
                     /locus_tag="RHV13_001518"
     CDS             complement(12597..13457)
                     /gene="repA"
                     /locus_tag="RHV13_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HDV1241071.1"
                     /translation="MNQSFISDILYADIESKAKELTVNSNNTVQPVALMRLGVFVPKP
                     SKSKGESKEIDATKAFSQLEIAKAEGYDDIKITGPRLDMDTDFKTWIGVIYAFSKYGL
                     SSNTIQLSFQEFAKACGFPSKRLDAKLRLTIHESLGRLRNKGIAFKRGKDAKGGYQTG
                     LLKVGRFDADLDLIELEADSKLWELFQLDYRVLLQHHALRALPKKEAAQAIYTFIESL
                     PQNPLPLSFARIRERLALQSAVGEQNRIIKKAIEQLKTIGYLDCSIEKKGRESFVIVH
                     SRNPKLKLPE"
     gene            complement(13775..14167)
                     /locus_tag="RHV13_001519"
     CDS             complement(13775..14167)
                     /locus_tag="RHV13_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1241072.1"
                     /translation="MSDKVTVKQTINKATSIYKIEHITVGKPGSEQYRHAFELADQLG
                     LKHPDCIEHVFPTYADEQCTHVLTEEDFFSTEEREGVDRCIGVICSSVSYELFPNVHE
                     NGGIGYQFLYEGDELKCYEHGLLIESVE"
     gene            complement(14179..14319)
                     /locus_tag="RHV13_001520"
     CDS             complement(14179..14319)
                     /locus_tag="RHV13_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1241073.1"
                     /translation="MCRMPEFSSEYFDDLPAYILDTETMLMGINRKNRNVNDYNRAIS
                     GN"
     gene            complement(14345..14767)
                     /locus_tag="RHV13_001521"
     CDS             complement(14345..14767)
                     /locus_tag="RHV13_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ppfA"
                     /protein_id="HDV1241074.1"
                     /translation="MSFFSTLKTALSLKEKLAATGVLVLICALVGAGFAWERHQLKQA
                     MEKIGSLDQAVKERDKSIMDLNQTIETMNKAEQHFHSQEVKNESEQAKYADRQMERKA
                     EVQKQLVAAGNVRQRIPADTQRLLRESISEFNADADKG"
     gene            complement(14807..15595)
                     /locus_tag="RHV13_001522"
     CDS             complement(14807..15595)
                     /locus_tag="RHV13_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1241075.1"
                     /translation="MLEKDYQLSAYKKLAAAGGMKTPGAITSARNSANTAKLLAEELT
                     GLILDTIVYPDTITSYVSTIRTTATGLTNIGGLATQHADLLAGYADLSMLLQLDIGWD
                     VYCRANEREVSELPISIAIGDVTITKSLEDAVNALNTSSLVAAMGEINQTLNTGSGSS
                     SGSGSGGGTATPPPALTEEQIESLKVATEQFGVVFNQTTAPTTALQQQYERANESANV
                     AITAYNHAIGTALAEASANKASTASAIAALVPDSVLDELNKAAQ"
     gene            16058..16342
                     /locus_tag="RHV13_001523"
     CDS             16058..16342
                     /locus_tag="RHV13_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001177860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1241076.1"
                     /translation="MQMELISRKEFDSRVTSGELDNLQAIKVKEGFCLIGNQSGTNRV
                     FMLRRTDLKPFVWKNEIGPSSYAQTRGCHNLAFFYKDELSVVDIQGLQHV"
     gene            16335..17240
                     /gene="rdgC"
                     /locus_tag="RHV13_001524"
     CDS             16335..17240
                     /gene="rdgC"
                     /locus_tag="RHV13_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001702258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HDV1241077.1"
                     /translation="MFKHWKNITIYKLSREADLTDLEDKKKMILFTPCGSQDMAKFGF
                     VSPFGDNSEVIAMHGNGFILVEAKRETKILPPPVIQRAIQEKIEKLEQEQARKLKKTE
                     KDSLKDEVLHSLLPRAFSKFSVIQAIYDGSTKRIYINASSRQAEDMLALMRKSLGSLP
                     VVPLSVENPIELTLTDWVRDGSAPQGFQMGDAAELKAVLEDGGIARVKKQDLGSDEIS
                     THLEAGKLVTKLALDWQNRIKFTLDHNFSLTSVKFADELLEQNSDIDSEDVAQRLDAD
                     FFLLTSEISCLVDALVNALGGEAKQ"
     gene            17237..19255
                     /locus_tag="RHV13_001525"
     CDS             17237..19255
                     /locus_tag="RHV13_001525"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HDV1241078.1"
                     /translation="MKELCYGSVCSGIEAASIAWEPLGMRPAWFAEIESFPSAVLAHR
                     WPHVANLGDMTKLAKKVLAGEIESPDVLVGGTPCQAFSIAGLRGGLDDERGALTLKYV
                     ELANAIDDKRAESFLKPAVIVWENVPGVLSSADNAFGCFLAGLAGEDVPFEPGDRPES
                     GKSNAFWRWDGKTGCHVPKWPQCGCIYGPQRKVAWRILDAQYFGVAQRRRRVFVVASA
                     RTDLDPATVLFEFEGVRRNIAPSRGEGKETTRYTSDIAIRSCDDTNIVAMAHGQGGAE
                     IKTDNSAPTLTCNHEAPIVLLGDGRMRRLTPVECERLQGFPDGHTLIPTEKRKKVSSD
                     ELAYLRKNSPDLNEEEAAMLAADGPRYKAIGNSMAIPVMRWIGERITKAACRQNEGRE
                     TKERKVKPAAEFERSIFKWAGGKFGVLEQIFRYLPEGKRLIEPFVGGGAVFMNAGYQE
                     NLLNDVNADLINFYKTLQREAHSLITLAHRFFQDYNTQEGFLAVRNAFNKQVYDDLHR
                     AAAFLFLNRHCFNGLTRYNQAGEFNVGYGKYKTPYFPLQEMEAFLGAEGRSEFVCGDF
                     AAVIEAAGEGDVIFCDPPYEPLPNTEGFTNYSGHDFKFEEQKRLVSLLTDAHRRGAKV
                     LITNSGAPNIRELYHDSGFRVEHLFARRSVSCKGDTRGVAHDVLGILL"
     gene            19929..20004
                     /locus_tag="RHV13_001526"
     tRNA            19929..20004
                     /locus_tag="RHV13_001526"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19962..19964,aa:Asn,seq:gtt)
     gene            20007..20082
                     /locus_tag="RHV13_001527"
     tRNA            20007..20082
                     /locus_tag="RHV13_001527"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20040..20042,aa:Thr,seq:tgt)
     gene            21032..21859
                     /locus_tag="RHV13_001528"
     CDS             21032..21859
                     /locus_tag="RHV13_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HDV1241079.1"
                     /translation="MKKTLISAAIILGSLCLTGCDRVEPGNVGIKVNKLGDDKGIGEV
                     VGVGRYWTGLNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPAKVTTV
                     FQTYRKGVDDITDTDLRQKVADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQREQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPGVMELEAINKWNGTLPQYMTSNTAVPFVPVK"
     gene            21887..21962
                     /locus_tag="RHV13_001529"
     tRNA            21887..21962
                     /locus_tag="RHV13_001529"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21920..21922,aa:Ile,seq:cat)
     gene            22249..23613
                     /gene="dnaB"
                     /locus_tag="RHV13_001530"
     CDS             22249..23613
                     /gene="dnaB"
                     /locus_tag="RHV13_001530"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDV1241080.1"
                     /translation="MSASPLESMPNSLSAEQAVLGGLMLDNCRWDEVADRIVADDFYT
                     SAHREIFSEMERLLSHGKPIDLITLAEALEQNGKLERAGGFAYLAEMSKNTPSAANIC
                     AYADIVRERAVVREMISVANEIAEAGYAQDGRGSNELLDMAERRVFEIAEKRQKSGSG
                     PKDIASILDATVSRIEELFQRPHDGVTGLDTGFTDLNKKTAGLQPSDLIIVAARPSMG
                     KTTFAMNLVENAAVRNDKPVLVFSLEMPSHQLMMRSLASLARVDQTRIRTGQLNDEDW
                     ARVSGAMGILLDKQNIFIDDSSALTPTELRSRARRVYKENGGLSMIMIDYLQLMRVPE
                     LQDNRTLEIAEISRSLKALAKELQVPVVALSQLNRSLEQRADKRPVNSDLRESGAIEQ
                     DADLIMFLYRDEVYHPDSEMKGIAEVIIGKQRNGPIGTVRLAFNGQYSRFDNYAGADW
                     QEDY"
     gene            23613..24611
                     /locus_tag="RHV13_001531"
     CDS             23613..24611
                     /locus_tag="RHV13_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1241081.1"
                     /translation="MQWNEEKPMNILIIGRKFEAISDVKTYTEMWAYNLACAFSEAGV
                     TLQYHRPYSPGVESPEDYVEAVLTAATACSAKAILAPGLRYFTTVPREIGMQLCRRFS
                     GWVAQVYDGSMLDSAPVDITFTVRDDTWRYLDNPGRLERHNRFNKHVGWAANQELFHL
                     ETKTDDILRIFVDHAAFDVSGFDHSLSILMNLQRLTVPYEARTLTDDGLVTIDPGNIS
                     VTPYRRTPVPATEFAAELRKSDVFIVTHPESLGLTVLEAAMCGALILTPPDCLPPDRL
                     ELVNHMVIKSRIDWDEVIARVDRVKNAEKVQCHTWSAIAEKMLETFITQKPSRGNG"
     gene            complement(24658..25290)
                     /locus_tag="RHV13_001532"
     CDS             complement(24658..25290)
                     /locus_tag="RHV13_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000535207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HDV1241082.1"
                     /translation="MGSLTGKYRAVVISVDDPKGLMRTQIRVVGMMDGLPDASLPWAE
                     AILSNANTFSPFLPGDKVWVEFPYNGDSRWPLIIGYAQDASGGAPNVPPEASGQGEGY
                     VPPEVEGAPAQPSTSAKKDFISSRNGLMEIRTAGGAWAVTHLKSGTTIGFNEAGELYA
                     ISQGPAFISSAGNLDIKSGADVALKAGGSMAIEASGNLSIKAAQVSVDKA"
     gene            complement(25283..26299)
                     /locus_tag="RHV13_001533"
     CDS             complement(25283..26299)
                     /locus_tag="RHV13_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDV1241083.1"
                     /translation="MATKTTTAPETDSKRTQLFLQSVSIGQNEIPREMIVGCTYVEPG
                     ELSGPQLMLMVRDSTAYVVNKLGVKFGTILTVSLGDPEGHGGILFSEEFFVLKAPRKD
                     DTVLIYAFSNPVRLLKVPSTSAQYFVDKPPSAVVSSLAPGLKVNADSFRKTSTYHLNV
                     GEKPTKVLQEIARDTGSMCWASRGTINFKSMEKMANAAPSLTYESANPNTSGFTISQF
                     NILNADYEYQRRHNYRMASYDMTKGVVYSGNQEDPIKFTSNPDPTALANYNKFILPRL
                     DMLVEGNAALTPGTTLKIVVHNTAGDGELDESIPDKMIVMSVTHFEDRFRFVSRAQLG
                     VVNG"
     gene            complement(26301..27086)
                     /locus_tag="RHV13_001534"
     CDS             complement(26301..27086)
                     /locus_tag="RHV13_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate"
                     /protein_id="HDV1241084.1"
                     /translation="MILNNQEWLLAIFKKKGLTPTGKLEFATIDGIDSALAQALNEAF
                     DSQVVSFNDRTNQSFREFLKRTPRDRITLGTFSDVKEWLSSFEADRAGRKDTASAGPV
                     NKLAMPLVNLSRSPAFSIYEGELCRDNYDEGHVTNENDEIEALVSTIPFSLEYSLWIA
                     SDEKESLGMVTTALAFWLRMYASLGQASFTHTANVGGYEIPVTCYIEGQKSIAFQDLT
                     TGTADNRLFAVGLNLTVVAELPILAYMQQTTGTITVKAKILEE"
     gene            complement(27073..27801)
                     /locus_tag="RHV13_001535"
     CDS             complement(27073..27801)
                     /locus_tag="RHV13_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000896792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV1241085.1"
                     /translation="MAGFFDDMFEDTEPSQQVTGDNLPDTESDPDIPGEGSELIEEED
                     IDAEIETDGVNVGNIVDPVEDNHLPNLDHGLLSDSGVRHRYQGHAVFNNLVRMDWLKA
                     IKLDPDSFDAVLYRAIPYRDKNAPETASEIIEPNQRIYDYQDPELITALDCPDEMDAF
                     YALYDGSDNTGISDSALILRLAAVNVPVGSMLEWLEQLSDGTTIRRFWYIHKIFNYGT
                     ARVGSLFYCVPSRAFEGNFIGDSE"
     gene            complement(27805..29022)
                     /locus_tag="RHV13_001536"
     CDS             complement(27805..29022)
                     /locus_tag="RHV13_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV1241086.1"
                     /translation="MPTEYARDNLGRYQTDGLSAKDFNKVFDLIRKQQRQNRRNARRT
                     LTPRIMGMRNRELEAFLSLGKKKDGTYFTPEDIRSFNTSRQAHKTKFKSTVPGITYAQ
                     LVAQSTSIDIKRANNKVSDGTGIKAATFLGLKHNLALISVNASDESVHQHHRVRIRFE
                     EWDKAVEEIAEDGAKKARIAADLCKGRVSFDCDCGRHQYWYRYMATAGNYAVAPPKEY
                     AFPKIRNPDLTGVACKHVLHAMTRFQSPTWHKAIIIALEKAAEQVAFGDDKRKTTTYF
                     KGELAKSLARNRTTTTDQAKAAREYELYLKSQDALGKKLRAKDSATDNVRRLLKKART
                     TANRKNAELKASRVREAQARAEADALKKALQTQANNLIKFFMSQGMDKAAATAQARSI
                     LETQINEARKRKG"
     gene            complement(29032..29409)
                     /locus_tag="RHV13_001537"
     CDS             complement(29032..29409)
                     /locus_tag="RHV13_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000235789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1241087.1"
                     /translation="MATSITTTQSTRQYPLSRYDDRNIADPILRAELRKEVMLMCESN
                     DKNLTIYYVLPDEQYRPDLLAYRMWGIAELRWVVTLAAGLEDESQGMTVGKKLKLPPA
                     TWIREMIRHFQYDGQVIGTLSIA"
     gene            29556..29801
                     /locus_tag="RHV13_001538"
     CDS             29556..29801
                     /locus_tag="RHV13_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1241088.1"
                     /translation="MAERVDDAELSMNQLEALKDMAIDNIRKQAQVVSQVFTGKCRYC
                     NESIESGIYCDAECAQWHREEQAAKQRKYGMRPAGFD"
     gene            29804..30382
                     /locus_tag="RHV13_001539"
     CDS             29804..30382
                     /locus_tag="RHV13_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HDV1241089.1"
                     /translation="MLRFTEEEFQAFSERRNKGRSRPKTKKDPFLSLAPVKEVSPHAK
                     ALAALAKNPDLRDGNCEHFEQVFIFDYFERKHPDIYELLHATPNGGKRSKATAGKMKA
                     EGQKKGYPDMSLDKACGIYHGMRIELKEPNGKAPTKEQIAWMRRLREEGYYVVLAYGA
                     EQAITAILEYISLKKGEAIEHVLNGDKWLHAA"
     gene            30449..30604
                     /locus_tag="RHV13_001540"
     CDS             30449..30604
                     /locus_tag="RHV13_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="norphogenetic protein"
                     /protein_id="HDV1241090.1"
                     /translation="MSSKVNYESLASVMPRNEQETDAVVDPVIAEMNARLEAEFAAEN
                     EHTTQGD"
     gene            31106..31732
                     /locus_tag="RHV13_001541"
     CDS             31106..31732
                     /locus_tag="RHV13_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="norphogenetic protein"
                     /protein_id="HDV1241091.1"
                     /translation="MFPKNKFRGSDRVIIVGSGPSAANFVAPRGVPIIAVNGAIDWLN
                     RASYFFTLDPSPDNMRRVGRGRRRRGVCYCMALPDVKEREVRDGVLCFRRVAERGMEP
                     KNTNSPEWWAWRWSAHFGLCEDENEIASGNSAYGALNLAFHIGFKHVALVGVDATQEL
                     RVHSGGTPKNLSHLPLLFQSAREQIDVVSCGKMGGIPQMTLKEWLKNT"
     gene            31729..32406
                     /locus_tag="RHV13_001542"
     CDS             31729..32406
                     /locus_tag="RHV13_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDV1241092.1"
                     /translation="MMAPTIYHRIDGTKYRNVWVVGDLHGCYTRLMSELHRVDFDPAQ
                     DLLISVGDLIDRGTENVECLELLQMPWFRAVMGNHERLMIDALSPDGNVNNWLMNGGQ
                     WFFMLDTDQEILAWALVELVKRLPYIIELNTGQETIVIAHADYPDNEYQFGKEVPLFN
                     VVWARERISDSMDDIGGEISGADRFIFGHTPVKSPKTFWNQQYIDTGAVFCGNLTLMK
                     VKGDGAA"
     gene            32403..33104
                     /locus_tag="RHV13_001543"
     CDS             32403..33104
                     /locus_tag="RHV13_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1241093.1"
                     /translation="MKIALVFRSGGDYNASDVQWLVNQLPKGYEIICLTDLKRLHVPG
                     VKVVPLINQWQKCRGWWAKIELFRPDITDDLFYLDLDTVIAGDIRPILEHPPTSFTML
                     RDFYHPQYRGSGALWIPNSVKAHIWSSFWQDPEGWISRCVTTECWGDQGFLRKVMGDD
                     TPAFQDLYPGWFVSYKADVVEPGSKYASARYSRGNGALPKDCRIIFFHGKPRPREVSE
                     DWLPLISSFFERESE"
BASE COUNT         8510 a         7809 c         8172 g         8634 t
ORIGIN      
        1 cttaatttat gtatcgacta ttgatatcca tcttaaagtt acaatatccc tctgcattat
       61 ttttcatcac aaataatata tctattgcac gacactaatc tgctctaata aatttgtatt
      121 tttaagtcgc gaatgctatc ttttcacatc atattgacct tttaatcgtt caggcttata
      181 gtgccgccgt cgtagcaaat tctgcgaccg ggtttagcag cctgaatact tacgcggaca
      241 accgcagatt tccgatattg cggtattttt gtgtccgtaa accacgttac gcccgaatta
      301 tggtggggcg tgatggggag gcttcggcct gctggtttcg taagtgccag tctgctaacc
      361 ccgtcacgtc ctgccacctg tttagcagcg ggtcgcaggt tgtttatcaa cttacgaggc
      421 cgtaactatg gttaatgcca atccttgcgc acgccaagaa ttcatctggc gtttctattc
      481 ctgtaaaaaa caccactatc acttcgttat cgcagcaaca gaagacgaag cacgctctca
      541 attgcctgac ggcccctgca tttttactgc ccgtttttca actaactcgc gcaatccact
      601 tagttactgg agcctcccct tctctgccga cgttcagggg ggtttatgaa aaaacctctc
      661 gtcacccgta atgacatagc cgaagcgatc gccctgcata ctgcctgtat gccgacacgg
      721 gagatccccg gcgcaattgc caactatttc atgataacca gacgttttta tacccgaaca
      781 gataaggctg tgatcaacaa gctactgata gccgagatca gggattattt gattgaacaa
      841 ggacgtctac gttacgcaac agtggcagca gaaatgagaa aggaggcaca tagaatgacc
      901 ggtaataatt tgaatgttga aaaaacagca cctgttacgt cagctacgcc agcaccagcc
      961 gtgaatatca tccccaacac cggagacaca atcgacagcc tgacactgtt aaagatggtc
     1021 aatgaagcgc gtaagttatg tggggaacca gaggttcgga acaacaaatt catcgaaaaa
     1081 atactcgacg aattagaagg tgaggacggt tacacaaaaa gtgcaaccgt gccgccaggt
     1141 ggcggtacgc ctatggttgt cataaccatg acctacaaac aagccctgcg agtcgccgca
     1201 cgcgaatcaa aagccgtccg ccgttcgctg atcgacaaac tggaagaatt gcagcaggca
     1261 aactccccta ccccatcgat cccccaaaca ttaccagaag ctctacgcct ggctgccgag
     1321 ttagcagaac agaaaatgca gctggaacaa cagctggtgg ccgctgcccc taaagtcgat
     1381 tttgccgacc gggtatcagt ggctaatgga atcctgatcg ggaactttgc aaaggtcgtt
     1441 ggacttaagc aaaacgccct tttctcatgg ttgcgccaga acggcattct catggctttt
     1501 ggtgcgcgca aaaacgtacc gcgccagcag tatattaacg ccgggtattt cacagtgaaa
     1561 gaagtggtgc tggatgatga aaatggctac cagatacggc tgacgcccca attaacgggt
     1621 aaaggccagc agtggttaac tcgcaagcta cttgatgctg gtttgttaaa accagtagca
     1681 ataggttaac aaaagaaaaa aacctgccag caaactggca ggtttctgag cagatcgtcc
     1741 aacccgatct ggatcgagtc agaaaaattt gctctaataa atttcgtttt ctaagtgcaa
     1801 agaatcacca tttcgagctg gtgattgaag gttgatgcaa atttggagaa aaaatgcaac
     1861 aaacattcaa tgcggatatg aatatatcaa accttcatca aaatgtcgat ccttcaacca
     1921 ctctgcctgt tatttgtggt gttgaaatta cgaccgaccg cgctggccgt tacaacctta
     1981 atgctctaca cagagcgagc ggactcggtg cccataaagc gccagctcaa tggctaagaa
     2041 cgctgtcagc caaacagctc atcgaagagc ttgaaaaaga aactatgcag aattgcatag
     2101 tttcgttcga aggccgtggc ggcggcactt ttgcccatga attgctcgca gtggagtacg
     2161 caggctggat ttctcccgcg tttcggctga aggtaaacca gacatttatc gactatcgag
     2221 ccggaagatt acaacctgct attccgaaga gcctcccaga agctctccgt ttggctgctg
     2281 acctggcaga gcaaaagcaa cggctggagc aaaaaatgct gatggatgca cctaaagtcg
     2341 aattcgccga acgcgttgct accgccagcg gggttctaat cggcaactat gccaaagtgc
     2401 tcggcctggg ccaaaactat ctcttcacct ggttgcgtga taacggaatt ctgatcgcaa
     2461 ccggtgaacg caggaacgtc cccaaacaag aatacatatc ccgtgggtat ttcaccctta
     2521 aagaaaccgt gatcgataca agcaatggaa gcaggatttc tttcacgact cgtataaccg
     2581 gcaaaggtca gcagtggctg atgaagcgat tgcttgatgc tggtgtgctg gtacctgtcg
     2641 cggcaacgcg ctaacagacg tagtaagaac caccagcatt gtaatgctgg ctaaagtcac
     2701 tttcctgagc tgtataacga tgagcgattt tactttttct ggctatgaat tggcctgctt
     2761 tgtaacacac tccggtctat cccgtagcgc cgggcatatc ctgtcgcaat gtgcaaatct
     2821 cgcggcaaca accagtgaat acttcattca caagcctcac cgcctgatcg cggcagaaac
     2881 tggttatagc caatcaaccg tcgttcgtgc attccgtgaa gctgtaaaca aaggaattct
     2941 gtctgtagag attgttatcg gcgatcaccg tgaacgtcgc gctaacctgt accggtttac
     3001 accatccttt ttggccttcg cacaacaagc caaaaatgcg ctgattgaaa gcaaattaaa
     3061 gatctcttca gcggcaacca aggttaaagc tgttctcgct aagacattgg ctttatttaa
     3121 ttttttatcc acacccccat gtcaaaatga taccccctcc ccctgtcagg atgacgtggc
     3181 aataaagaat aagaagtcac aagttaaaaa aacaaaaaga tcagtttccg gcggtgccgg
     3241 aacgaccaga ctcaaaaaat tgacttcatg gatcgctgag gcaaaagcaa aggctgacaa
     3301 tctgcggtta tccaaaaaac gcgctcaaaa acatgagttc aagcagaagg tagaggcggc
     3361 agcgcggaaa tatgcttacc tgaagaacaa gcgttctcct gatattggcg gggtatcaaa
     3421 cttcgataat ctgccgcatt gcatgacggt aaacgaagct cttaatgcgg ttttagccaa
     3481 aaataaagat aacgaacaat ggggtatacc ggcaggattc agagggtgat agattgctct
     3541 aatctggagt cacctggcgt tttcagtttg aggtcggaga tgcaatctga ttttttacag
     3601 ttagcgatcg cttttgcagg atatgtttgt attggcttct gtgtatacat gttcagccga
     3661 aaaatgcttg tcgatatcga ccgcaaagaa caagcagagg agatcttagt atggattttc
     3721 tttggcgcgg tctggccatt agggatcatg tttgctgcaa catttcttct gatgtggata
     3781 ttcacccttc caggtgattt ctatagaaaa aaagccagac attgatacaa tcgttgcggg
     3841 tgcttgaggc tatctgcttc aggcattacc cgaaaagcag atagaagaaa gccccagata
     3901 acattacgcg tcctgcaaga cgcttaacat taatctgagg ccatatctat gcttagcata
     3961 cgtagattag cctcttaccg accaaaaggt caaggagaag caggctatga agcagcaaaa
     4021 agcgatgtta atcgctctga tcgtcatctg tttaaccgtc attgtgacgg cactggtaac
     4081 gaggaaagac ctctgcgagg tacgaatccg aaccggccag acggaggtca ctgtcttcac
     4141 agcctacgaa tctgaaaggt aagagacctg gtggggagag atccccgcca ctcttagtgt
     4201 gtcaggtatc ctcaatgcac cctttcatct ccaaataaaa aagctcccga aggagcttta
     4261 aaatacaagg gatgactctt aatcccactc aatccagttg tagacgatac gaagtgacgg
     4321 gcgcacagcg gcagtcacat cttcggtact aaagtcgatt gcatcactgt agattttgca
     4381 gtccaacatt tcaattgttg tagcagcttt tgtcacagcg ttaaccccgg aagatttgga
     4441 ttcaggggta gcagccatcg tgatatcaac atagtccttc gccgcaatgc gatccttgat
     4501 gaactgaaga atatcgcctt cgatagtctc cacgcactgg acctggattt ccccagagtt
     4561 tcgaattggt ccgtgctggt tgaacttcac accattcgga ccatagtcct ccacatcctc
     4621 gcgggtcatt tcaggaattt gcgacgtgcg aaccagtacg ctgatatctt catggcctgc
     4681 aaaagtgagc tggaattcag aagataccag tcgttcgcct ttggccgcgt tggcagtata
     4741 gcggccctta ataaatttac ggtttccctt agtgttattg tgccccatat aaaatccttt
     4801 tactggaacg cccgaacaat atcggagctg ttatatatcg aagaaccggt caactggagg
     4861 ttgacggtgt ttttcaggaa atgcccattg ctgtccctgg gcgcatcgag atcgaaactt
     4921 atgtcctgga tagcgacatc aatgatgttg atccggcgac caatgtttag cgtcacgcgc
     4981 tccgggattc gaccaccaat actggcatct ttaagttccg ggctaatcat cgctgacaat
     5041 gcggcgatag ctcctgaaac ctccgtgaat gggtcaaaca aagcgatgaa agttactggc
     5101 agcgtgaaag tcggcggtgt tcccccttcc caaaccatta agctgttcca acgggcaacc
     5161 gacgttgttt cagtaccaac ctgcgcaaaa ccactgaagg caccagcaac agatcccatg
     5221 gacataccgg taaacggcgc ttcccaattc tgggccatgt tcattgccgc cccctggctg
     5281 atatatccgg taacctggta ctgagagttc gttaaagtaa ctttcagaaa tggcgataca
     5341 ccgtcagcct ggctgtaaac cccataagga ataggtgcca ttcaagttaa aggccggagt
     5401 tctccggcct cctcctttag ccaaggcgct tacggcgcag tttcattgac tttttgcggg
     5461 caagttttgc cgcgccggtc tgggcttttc gacgcgcttt tttcagcgcc gatttttgag
     5521 ccgcagtcag acgtttttta cgcaggcgtt tacggatgag tttgatctca ccgttacgaa
     5581 caaccttctt aaatgcttca gtcagcattt catcagaagt gccagcaaca acaaacgccg
     5641 cttccagttc gtcgcggtcg tcgctatcta aaccagcgat agaggcacca acatcagcag
     5701 ctgcgtcgtc gtcttcatcg tcagccagtg cttcgatcag gtcatcatct acaccgcatg
     5761 ctgcgaggaa gtcagcaaca tttgcccatg cttcgttata ggcatcgtcc tgttcttctg
     5821 taacttcgga gtcgtcgtca tcagagatac cagcgatagc ctgaacgaaa ccatcaaggg
     5881 agtcgaaagt cagatcaccg ctatcagccc aggcgaaaac ggcgtcggcc gcatcactca
     5941 acgcattttg catagcactt cgatttgcag cttccagaat catctggtgc gcctgttcga
     6001 cggtccattc tttaccgtct ttcccttcca ggatttgctc aggagccggg gcagatggaa
     6061 cgttatcgtt agtctgtgcc gccggttccg gattattatt aataaccgga tctgttggcg
     6121 gttcggcgct tgctcgggca gactccatca gctgcacagg atcagagttc aaagcgaaac
     6181 gagacagtcc attccccaaa aatgccccgg attgaaaaaa gtttttgctc attgtattcc
     6241 cttacttaat aagcagcggt acgccctgga tacgacgggc tacgccagtc gggcagcagg
     6301 cccagactac ttcccattta tcgaattccg cctgcgtaac tttcagcaca tacggttctg
     6361 taccgtcagc atcaggatca cgaggagcca ccagagcgcc ggaggcgaca aagcgatcta
     6421 aaagtttggt catcccttta gtcaggccag ccgcagtaat accgtccggg ctatgcttca
     6481 tctgtcgggc taactggaca aagaaacggc tgattgcatt catcagggat gggacgtgct
     6541 ggaagtgcag atagttatcc tgcgtgcagc aagttaaagc atcgtcgatg atcatctggc
     6601 cagaggtgcc aacagatact ttattgagac ggcccttgac cattgcttct tcgtccgggg
     6661 tatcttccgg atacagcggt tgaattgacg cacgagcaat gacggcacgt tcttcaccag
     6721 ccggtgagta atgccaaccg ccgacatcag agtttttctt gacgccacga gctttcgccg
     6781 catacgccac gccagacaga ccaaagacca cacgggattg ggtccatttg tctttgcagg
     6841 agaacgggta gtgatagaca gcacagctta cataatcggt accaagtaaa ccggtatctt
     6901 caacagcaga gatcgcttcc gtgtacgtca atgtcggttt gacatcaaag aagccatcaa
     6961 tcaggcgatc tgcacagatt ttacctaatg cggtgatagc cgcattgtca tagcagccca
     7021 agccaagaac agcggtgtac atgtacggcg cattattcag cactttaacc gcacgcaggt
     7081 acgcagcggt tgagattttc gactgatcgc cgttggtacc gccagtgaac gccaacgatt
     7141 ttttgtttgt tactttcgct gtcgaaatca gctcttcatt aacaaccgcg cgcagatatt
     7201 tagaacgggc ttccagagcc gtaggcagat aacacaagcg gcccatgtca tctttcgctt
     7261 cttccgccaa agacacagtg tgtgtctcca gggtcgttac cacgccgagc gaagtcgtct
     7321 gggtcagttt taagaggaag cgttcattac ccgcgctgtc cgctgttgcc gtttcgatgg
     7381 ttaactcacg ggtaggtgaa atacacggat caccatcatc aacgtagata gcaaaggctt
     7441 cgccactatc aagttcaatt tcagaaccgt atggcaacgc actgtaagcc ggttcgcctg
     7501 attcatcgaa cataataatc gggaacttcg catcatccgg aacagcgcga acaacataac
     7561 cagacgtttg ctgaatagct tcgtatacat ggcgaattgg ttcgaactgt gagccggaag
     7621 acggcttcag cggttcgccg agaacatctt cgtaattgga ctcagtaacc gcaagaacag
     7681 taaacggctt gccacgcgca aatacgccaa taccagccca caagctgcta tttaatgcaa
     7741 caccggtaga taacgtcgca tcggcattga tcgggctaac cgcgacgccg gatgcattac
     7801 ctaatgactg ttgaattgaa tattgagaca taactttccc tgttatgcgc cccgcacggg
     7861 ggcgctatgt taaacggaga acttcccctg attactcaga gtcaccggca tcaatcgtgt
     7921 cgccgctaat gaagttaagc ccgccttttt tggccattgt cagcgttaca cgagtgaagt
     7981 aatcagcgcc gttgcgtggg tgcatatcgt tgatagccga accccacagt gtggtacggt
     8041 tgaccagcgc cggagtggtc ggatgctgga acgggatagc cgggacagca tcaccagtca
     8101 cgaagcctgc tttacccgga ttttcatcac ggacgtagca cagcacatcc atcgagctga
     8161 actgaatgtt ctctgtcgtt aagttcttac aaataccagc aggtacttcg tacactttca
     8221 cgttaccgaa cagggtaccg atgtagtgaa catacggagt ctggatatag tcttcggctg
     8281 gctggaagaa atccttcggc aactgtttga agaaagatgc tgcatcagca ccagcaaaca
     8341 tccccatcgc accagaagat ttaacgcgct caataatgtc gcgatataca gtctggaatt
     8401 tgccacgaat gatggttgcc catacatcaa aggactggtt aaccggcaga gcgatgtcaa
     8461 aggtgtcggt cgcaagagta cgccagatca tgatgcgaag acgcagcata tcctgttcat
     8521 gagacaggta ttccttcagg gtgcggaact gtagggaacc caggtccaga ccaaattcac
     8581 gctgtgcttc atacgccgcc tgtaccgtgt gctcagccgc gataacgaac tggcttggga
     8641 acagggtgta tttcttcatt tcgtggttga tcagcgggat cagctcagga gcggcttcaa
     8701 tattgatttc cgtctcaatt gcgatctcag tgcctttatc cggcgctttg gagaacgaca
     8761 gggcaatctg accaatgttg tagttcagag agcaggtaac agtgatttgc tcaccagcag
     8821 cattagtaaa cgagtgaagt aggctgccgg aaccgttatc aacaacagac ttaatacggt
     8881 taacgtagat gttagtgcga ccttttcgga ttggtacatt ctggccttcg aagtcttcca
     8941 tcttgaaggt tgcggttttg ctggtgccat cggagcttgc caccagcaca tagcggcgac
     9001 gtaattggct gtacacaccg acggattgca tgtccagaac atcaccagca gcataagaac
     9061 caaaagagga acctgccacg ttaaagactt catagatgtc ggactggtca cgcgtaaccg
     9121 gaatgaaggt acacgcatca gcggtagctg cccccaactg aacaggcagg atcatcgcga
     9181 ggaataaagg cagacgcata acaccgtcag aaacgctcat catctctgct gcgacggatt
     9241 ccagcatcgc tttattagtg gcatccatgc tattgcgggt ggactcaatc aggcagtttt
     9301 ccagcgtctg gtggcaggag gccagaattt ccggacgcgg catagattta tgtgctgcgg
     9361 cgtagtcagc cagtgcactt gcccacgctg tagcgatttg agcggtggca ttatcagaga
     9421 tacccgcaaa aaccgggtct ttacgtgcag cttcaaggat agatgcggca cgcgcggcat
     9481 catctttaat gaattggtta tcagtaccga actgcgcagt gcttgcccag ccaagcacag
     9541 ctttagagcg ttttgcgata tctgcaatac gattctggta ttcgcgtaag ttactcaatt
     9601 tactcttcct taaacacaag gcacttgtgt gaatcccttt tcggaagaga ttttattgaa
     9661 agtcacttgt tgactttctc gtgacaagca atttttttat ttttttcggg agtaggggag
     9721 gaaggtaaaa tccaaggtga aatcgtggcg atttcacctt gaaattttag atggatttac
     9781 tttaaaaaca gttggttaat agtgaaattt gaatggcgaa aatttaaggc ttcggctttt
     9841 tatcgaggct ctttctaagg atatgcccaa tcatcctgtc gagttcttcc tgtagctctt
     9901 ttgaaagtcg attaaactca taagaaaatg cacggccttt cacgcgcttc cttgcaaagc
     9961 gatccttgtc ctcaaatttc cataattcag taactacgga cttatcttta gaacctttat
    10021 ccgtgagtag tgaggcttcc tttgttatca agcgcaggat tttattttta acttcatctt
    10081 cggccatttc ttcaatggat aagatgtcgt ttatttccgg ggatatgttt tgaataagct
    10141 gatcaaactc taaattcttg ttccccattt cgtcgccaac agcacaaagc gttttgtagt
    10201 ccgaaaaggt taattccgac tgaacaggga aaagggcgac taattcttcc ggagcactcg
    10261 ctgcctggag agcacgcgtg accttcgcct gagacagccc ttctttggct gcaatatcct
    10321 tctgactcat cccatcattt ttcattcgca tcaaacgcag acctatttct cgaatgctgt
    10381 gctgcaatgc tgtctgaacg tctttcgcta aattttgcgc ttcctgaacg ctgatctcct
    10441 ggtccgtgac taaaacccgc aaccctacgt tctctaagat ggcagaagct cgacgccggg
    10501 aaccatccaa aatttcaatt ttccctgtag cccgtctaac acctattgca gggtaaaatt
    10561 gctgatgctt aatagtgctt cggatacttt ttaatgattt tggcgtaaga gatgcctggt
    10621 cacgcccgtt gttatgctga tcaacaaagg tatcgctttc tacctggttc ggaggtatta
    10681 cctcttcaat aaatgtggcc tggcgaccag ttgataactt gaatacctgc tcgactcgat
    10741 cgccagaggc tgaagaacta tcaaatccgc ttaatattga aggattaagg gttcgcccaa
    10801 ttgttggtct gtttttcttt gacatggggg tttcttactc ctcagttaga tctgataaat
    10861 tcaatacggt caaaaactgc tttagcaaaa tcttccgcgg caattcgcgc gttcttcaat
    10921 gcatcagcac taccaacata cgttgccggg ttagctgaaa taacagtgtc aaaagactcg
    10981 ccgcaacgtt caaaaccgtc aaggcgaggg aggacgacat cgagcatatc cccaccgaac
    11041 acttctttag ccaggctatg gcaatactta tgatctgcct tgttactcaa cttggacata
    11101 aaaccaatgt tagtcgcaag ctggcactcg cagccttcat ccgaaatgag ttttaccaac
    11161 tcaggaaggc gggcaacgta tttaagcgat gagtggaaat cgacagttgc aggcggcaga
    11221 ggtgtaaaca gtatattggc cgaggccaaa gcatttttca ggaaggcgtc aaggtgagga
    11281 ccactatcaa cgaggataaa gtcataatcg ctcttcagct tatcaatcac attttctttc
    11341 aggactgcat ggatgttctg acccggtaga tgctcattgc acagctctct ccaatcggat
    11401 gcaataaagg catcgtcaat cgacgcaggc ataacgtcaa ccccaggtac aacagaagga
    11461 acaataaact cctctaacag ctcttcacgg cttacattct gcaacatagc ctgtgcagat
    11521 gttgcgttta cgataccaat agagtgttta tggcttaaaa acatcgttgc tgaagattgc
    11581 ggatcaaggt caataaccag aattcttaaa tcttccatca gaagatgagg gtgggcacgc
    11641 attgcatgcg ccagagaaac cgtcgataca gtttttgaca caccgccttt aagattggag
    11701 atgaaaatca catacgcttc gctgtagcga tcccggtatt ttggcactcc gcgatgttca
    11761 tatatgtcaa tgatgttctg aattgacatc gcatatttca ttgaagagcc agcagggcgt
    11821 ttatcgaaaa cataaccctt ttcttccatt tcacttacgg catagtcaac gttcgctcga
    11881 gtcagtagag gcaattttgc cagtgccgct ttcgcataga cctggtaaaa ctcgttcgcg
    11941 tgtagctcat ccttttgcaa ctgtacttgt tcagtcagaa cattgagcat tctgtttgct
    12001 ctttgagcaa ccttgtgaag ctggctggaa tcactcatcg aaagtcatcc tttatgctgt
    12061 atttttgaat ttaattaaaa atgctgcata aaataataat gtatgcgtag atgcttgtac
    12121 atagcattct ctgcatgttt actcctttgc caagtgtgtc acaacatgct ttggcatcct
    12181 ccacgcataa aggacgcgga tttcttctat gttcaggttg tagcctgaat cattttggtg
    12241 atttcctgct tcaacgagca gccaggcttc actctctatc aactggtgag aacggcttgc
    12301 ccggcagctt cgatgtgtgc tggagggaga ataaaagtct aagatgtgcg atagagggaa
    12361 gtcgcattga attatgtgct gtggagggat cgctggtatc aaatgtgtgt gctggaggga
    12421 aagacagaga attacatgtg cactggaggg aaaaacggat ggacagatgt gtgctggagg
    12481 gaaagtctga gcaaactgcg gggcgtcccc ctccagcgca catcaaaaac gggaaattgg
    12541 acaagccttc ccggcagcac acattttttt gaatgcagct gccatccagc acacacttat
    12601 tcggggagtt tcagctttgg attgcgagaa tggacgatta caaaactttc ccggcctttc
    12661 ttctcaattg aacagtcgag atagccgatt gttttaagct gttctatcgc tttcttaatg
    12721 atacggtttt gctcgccaac agctgactgc aaagccaggc gctcacggat tcgcgcgaac
    12781 gatagcggca acgggttctg cggaaggctt tcgatgaaag tgtaaatggc ttgtgcagct
    12841 tctttcttcg gaagggcacg caaggcgtgg tgttgcaaca gaacgcgata atcaagctgg
    12901 aacagctccc acagcttcga atcagcctcc agctctatca gatcaaggtc agcatcaaaa
    12961 cgcccgacct tcagcagacc agtctgatag ccgcctttag catcttttcc gcgcttaaaa
    13021 gcgataccct tgttacgcaa gcgtccaagt gattcatgaa tggttaaacg cagtttcgca
    13081 tccagacgtt ttgaggggaa accacaggct ttagcgaatt cctgaaacga taactggatg
    13141 gtgtttgagg acaagccgta tttgctgaac gcgtagatga caccgatcca cgttttgaaa
    13201 tcagtatcca tatcgagtcg aggaccggtg attttaatat catcgtaacc ctcggcttta
    13261 gctatctcca gctgggaaaa cgctttggtg gcatcaatct ctttactttc tcctttgctc
    13321 tttgatggct tcggcacgaa tacccccaag cgcatcaacg ctacaggctg cacagtgttg
    13381 tttgaattaa ctgttagttc ttttgcctta ctttcaatgt ctgcgtaaag aatatcggag
    13441 ataaaagatt gattcatatt actttttccg aattatgtgg atagttttta taagcggtga
    13501 taactacccg ggctttcccg tcagcacaca tcctatatcc cgccagcaca cattagcaac
    13561 ccgtcagcac acatttttat cccgtcagca cacatcgttt tccctccagc acacatcgcg
    13621 atacacttct aagccagacg tggcgcggcc tgcaacgatc agggatctat atggatctaa
    13681 ttgggatctg tatggaccta attattggat ctacccagtg gataatgtgg ataagtgaaa
    13741 aaccggccaa catagccggt tggaaggaag ggtattattc tacgctttcg ataagaagac
    13801 catgttcata acatttaagc tcatcgcctt cgtacaggaa ttggtatcca ataccaccat
    13861 tttcatggac attagggaat aactcataac tcactgaaga gcaaatcaca ccaatgcaac
    13921 gatcaacgcc ttctcgttct tcagtgctga aaaaatcctc ttcggtaaga acatgagtac
    13981 attgctcatc agcataggtc ggaaatacat gctcgatgca atccgggtgt tttaaaccaa
    14041 gctgatcggc aagctcgaaa gcatgacggt attgttcaga tcctggcttg ccaacagtga
    14101 tgtgctcaat tttgtagatt gaagtcgctt tgttgatagt ttgctttact gttactttat
    14161 cagacataaa aatccctttt agttaccgct gatagcgcgg ttgtaatcat taacgttgcg
    14221 attcttcctg ttaatcccca tcagcatcgt ttctgtatcg aggatatacg ctggcagatc
    14281 atcaaaatat tcactgctaa actctggcat cctgcacata aatgcacttt ttggggcagg
    14341 gtggttaacc tttgtcggcg tcggcgttaa attcgctgat cgactcccgg agcaaccgct
    14401 gagtgtcagc aggaatacgc tggcgaacat tacccgccgc aacaagttgt ttctgaactt
    14461 cagcttttcg ttccatttgc ctgtcagcat acttggcttg ttctgattca tttttcactt
    14521 cctggctgtg aaaatgttgc tctgctttgt tcatcgtctc aatggtctgg ttaagatcca
    14581 ttattgactt atcacgttcc ttaacagcct gatcaagact gccaattttc tccatggctt
    14641 gctttagctg atgacgttcc cacgcaaacc cagcaccaac aagtgcgcaa atcagaacaa
    14701 gaacaccagt agcagcaagt ttctccttca aagacaaagc tgtttttaat gtagaaaaga
    14761 atgacatgtc ttcctcctga agaaaaatta tcaatgaagt cctttgttac tgtgccgctt
    14821 tgtttaattc atcaagaaca gaatcaggaa ccaaagcggc gattgcgctg gctgtgctgg
    14881 ccttatttgc tgatgcttcc gcaagcgcgg taccgatagc atggttataa gcagttatgg
    14941 ctacgttggc gctttcattc gctcgttcat actgctgttg taacgcagtt gtgggtgctg
    15001 ttgtctggtt gaaaacaacc ccaaactgtt cagttgctac tttcagagat tcaatttgct
    15061 cttctgttag tgctggtggg ggagtggcag tgccgccgcc tgaaccagag cctgacgagc
    15121 ttcctgagcc agtgttaagg gtctggttaa tctcccccat agcagcgact aaacttgatg
    15181 tattaagcgc gtttacagcg tcctcaagcg atttagtaat agtcacatca ccaatggcaa
    15241 tagagatcgg cagttctgaa acttctcgct cattagcacg acagtaaaca tcccaaccaa
    15301 tatcgagttg aaggagcatt gacagatcag cataaccagc caacaggtcc gcgtgctgag
    15361 ttgccagccc tccaatattc gttaaaccgg ttgcggttgt tctgatcgtt gaaacatagc
    15421 tggtaatagt gtcgggatag acaattgtat ccagaattaa tccggtcaat tcttctgcaa
    15481 gcagttttgc tgtgttagca ctgtttcgtg ccgatgttat ggcaccaggt gttttcatcc
    15541 caccggcggc ggccaatttt ttatatgcgg ataactggta gtctttttcc agcatgatat
    15601 ctcctaactt acctgaacca ggccgtctcc ggctgcaacg gtagagccgc atgaaacagg
    15661 atcaccaacg catacgatcc ctttcccgtt gacggtaaac catgccctgg ttgatatagc
    15721 ttgcccgccg tgcgtgctgt tcccatcggt atgctgtgca tattgcttac catcaactaa
    15781 cacttcgact ccgttgactt taagtagtgg ttcgctctct acgggaggcc tggatgggaa
    15841 tcctccgtgc cccgaacaaa tgctgtcttt tgttgcaata cttgccacgt catcaccaat
    15901 gatttgctct gattttcgtt attttaactt aggttatttg tggtctgtgt ggcgtttact
    15961 cattgcaaaa ttgctctaat aaatattgtt ttttatgtcg tgttttcggt accattcagc
    16021 catcgccctt caatgggcat ttgtttggag tcgtcagatg cagatggagc taataagccg
    16081 caaggagttc gatagccgtg taaccagcgg tgaactcgac aacttgcagg ctatcaaggt
    16141 gaaagaaggc ttttgcctca ttgggaatca gagcggaaca aatcgcgttt ttatgcttcg
    16201 ccgtacggat ttgaagccat ttgtctggaa gaacgaaatt ggtcccagct catacgctca
    16261 aacgaggggg tgccacaacc tggccttttt ctacaaagac gagctttctg tggttgatat
    16321 tcaagggtta caacatgttt aagcactgga aaaacattac tatttataaa ctttctcgtg
    16381 aggcggatct gaccgactta gaagataaaa agaaaatgat ccttttcacg ccatgcggta
    16441 gtcaggatat ggccaagttc ggttttgtat ctccatttgg tgataattcc gaagttatcg
    16501 ctatgcatgg aaatggtttt atccttgttg aagcaaagcg cgaaacaaaa attcttcccc
    16561 cgccggttat ccagcgagct attcaagaaa aaattgaaaa acttgagcaa gaacaagcgc
    16621 gtaaactgaa gaaaacagag aaggactccc tgaaagacga agttctgcat tctcttctgc
    16681 cacgggcttt ttcaaagttt tctgttatcc aggcgatcta cgacggttca actaaacgta
    16741 tctatatcaa tgccagctcg cggcaggcag aggatatgct cgcgcttatg cgtaaatctc
    16801 tgggttctct tcctgttgtt cccctaagtg ttgaaaatcc cattgaatta acgctaaccg
    16861 actgggtacg tgatggtagt gctccacagg gatttcaaat gggggatgcg gcagaactta
    16921 aggcagtgct tgaggatggc ggtattgccc gagtgaaaaa gcaggatttg ggaagcgatg
    16981 aaatttccac acacctggaa gctggcaagc tcgtcacaaa gttggcactc gactggcaga
    17041 accgcattaa atttacactg gaccataact tcagccttac cagcgtcaaa tttgcggatg
    17101 aattgcttga gcagaactct gatattgata gtgaagatgt agcgcagcga ctggacgcag
    17161 atttcttcct gttgaccagt gaaatttcgt gcctggttga tgctctggta aatgcccttg
    17221 gtggagaggc taagcagtga aagagctgtg ctatggatct gtttgcagtg gaattgaagc
    17281 cgcgagtatt gcctgggaac cgttgggtat gcgtcctgcg tggtttgctg aaatcgagtc
    17341 ttttccatct gccgttcttg cgcaccgctg gccccatgtc gccaaccttg gcgacatgac
    17401 aaaacttgcc aaaaaagtcc tggctgggga aatcgaatcc cctgatgtgc tcgtcggggg
    17461 aacaccttgt caggcattca gtatcgcggg attacgtggt gggcttgatg atgagcgtgg
    17521 cgcgctaact ttaaagtatg tggagcttgc aaatgcaatt gacgacaaac gggctgagtc
    17581 atttctcaaa ccagccgtta tcgtctggga aaatgtccca ggagtcttgt catcggcaga
    17641 taacgccttc ggatgtttcc ttgctggatt ggctggagaa gatgtgccat ttgaaccagg
    17701 tgatcgacct gaatcaggaa aaagcaacgc gttctggcgg tgggatggca aaaccggttg
    17761 ccatgttcca aagtggccgc agtgtggttg tatttatgga ccgcagcgaa aggtggcctg
    17821 gagaatcctt gatgcccaat acttcggagt ggcacaacga cgccgacgcg tgtttgttgt
    17881 cgcaagtgct cgaacagacc tcgatcccgc aacggtactt tttgagttcg aaggcgtgcg
    17941 ccggaatatt gcgccgagca gaggcgaggg gaaggaaact accagatata cttcagacat
    18001 cgctatcaga tcttgcgatg atacaaacat agttgccatg gcacatgggc aaggaggggc
    18061 tgagataaaa accgataatt cggcaccaac tttgacatgt aaccatgaag caccaattgt
    18121 attgctcggc gacggtagaa tgcgccgtct tacccctgtc gaatgtgaaa ggctgcaagg
    18181 ttttcctgat ggacatacat tgatccctac ggaaaagcgt aaaaaagttt cttcagatga
    18241 actggcatac cttcgcaaaa actctcctga tttgaacgaa gaagaggccg caatgcttgc
    18301 agctgacgga ccgcgttaca aagcgatcgg caatagtatg gcgataccag taatgcgctg
    18361 gattggcgag cggataacca aggctgcatg tcggcagaat gaagggcgtg aaacaaaaga
    18421 gcgaaaagtt aaaccagcgg cagaattcga acggtccata ttcaaatggg ctggtggaaa
    18481 atttggtgtt ctggaacaaa tctttcgcta tttgccagaa gggaagcgcc tgattgaacc
    18541 tttcgtcggt ggtggagctg tcttcatgaa tgccggatac caggaaaatc tgctaaatga
    18601 tgtgaatgct gacctgatta acttttacaa gactctgcaa cgcgaggcgc attcacttat
    18661 cactctggca catcgtttct tccaggacta caacacccag gagggattcc tggcagtacg
    18721 gaatgcgttt aacaaacaag tctatgatga tttacatcgc gcagcggcgt ttttgttcct
    18781 gaaccgacat tgttttaacg gattgacgcg ttacaaccag gccggtgagt tcaatgtcgg
    18841 ttatgggaag tataaaactc cgtatttccc attacaggag atggaagcct tcctcggtgc
    18901 ggaagggcgg tctgagtttg tatgcggtga ttttgcagcg gtgattgaag ctgccggaga
    18961 aggagatgtc atcttttgcg atccgccgta tgaaccgctt ccaaatacag agggattcac
    19021 gaactattcc ggtcatgact ttaagtttga agagcaaaaa cgcctggtgt ctctgttgac
    19081 ggatgctcat cgtcgaggtg caaaggttct cattactaac agtggcgcgc caaacatcag
    19141 agagctttat catgacagtg gcttcagagt ggaacatctt tttgccagac gttctgtgtc
    19201 ttgtaagggg gacactcgag gtgtagctca tgacgttttg ggtatattgc tctaataaat
    19261 ttattagtgt aatatcgcct caatgaatcg tgatttatag agcgatttag ctgttagccg
    19321 cgacaggcgc ggcggtaagc atggctgggc ctagtcctcc cagacaaacc accgagttgc
    19381 caggttgacc atgcgcctaa gtggcaacgc cgaagtgcgt tacgagcttc cagtttgccc
    19441 atcttcgggt gggcgttttt ttcagggttt tcgtcatggt tagcgacttt gcggcggttt
    19501 agaaactgac cattaaagta aatgcaaacg atgatctgat gatggtagcg gcctaagaaa
    19561 ccagatgcca cggggtatga gtcgtccccc gtcaaaaaaa tcgaccgcag agtgtccccg
    19621 tctgtgtatt agggaacggg gaggcacaac aggtaagggc gctggtgtga ttaaccagat
    19681 gaacgagaag gggccatctg ttggtcagcg tcctttcctg ttgcgttttc ttttcagcgt
    19741 aacagcggtg cttaacagca ctttgggtac agttccacga atttacgggt atatcccgtc
    19801 atgctgaagg cgctaatcac gctggaagcc agggttgtgc atcccctgtt accgaattgc
    19861 agccagggcg cggtgcgccg aaaagcatac ggaggtggaa gccctcgccg gagacgtacc
    19921 cggcaagtga tggtgtagct cagcggttag agcggttgac tgttaatcaa cgggtcgatg
    19981 gttcaaatcc atccaccatc gccaatgccg gtttagctca gttggtagag cgcctgcctt
    20041 gtaagcagga tgtcagcggt tcgagtccgt taatcggcac cagcacaaca ggtaagggta
    20101 ttttgcgacg tcggagatcg ccgagcttgg cagagggttc gaatccctac gaagtaccct
    20161 taccgttgtg atgaagtgca gctctttgaa gcaaccagaa gataagcatc tggcttcaca
    20221 acataaaccg caggaacgac caataaacgg tagtccgtat ggagaacacc ccgttgagga
    20281 agaggcctgg ccggaaccgt aaccggcact acaacgttga gaacactggc gtaacggggt
    20341 catatcccaa tctacgaata aatgttgcgt tgcagcgtga caaccagtgt tctcaacatt
    20401 gtggtgaatg cacaggctga tgtgccgcaa ctacagtagt gcgcgctttg cggggcttgc
    20461 tacaaccctg tgtcggagtt cagcaccgac catcacagtt tgattctctg gcatgagcat
    20521 aacgctgaaa taagtccagc ctggtgcggc ccgatcaccc gccgttagct ccacgaaacg
    20581 gagcacgtaa caggtaagag cattctcctg taacgggttc atatcccaat ctacaggtcc
    20641 accaagaatg ctctttccgt tgcggtgaat gcggctaagc gcacgcgggg aaatggttat
    20701 atcagtccat tcatttctcc ttgtttcccc gtccacggtg gataaccagc caaaggacac
    20761 cgggaggcac ccggcaccgc agctttttta ttcgttaaat aatggagtga gaggatgcag
    20821 aacaatccga acaaatgtcg aacgctatgg gtgcggttat atatttatgc cgtcctctgt
    20881 ttgattgtgt cactggttct gtatgtttgg cttttgccaa atatgatctc atctaacagc
    20941 acaatacttg tattgttggg agtccttctc gcgctcattt acccggcttt cgcagtagtc
    21001 ttttttcgtg aaaaaaccag gaaattaatt aatgaaaaaa acgttgatta gtgcagcgat
    21061 tattttgggt tctttatgtc tgaccggatg cgatcgggta gagccaggta acgtagggat
    21121 caaagtaaat aagctggggg atgataaagg tatcggtgaa gtagttggcg ttggtcgcta
    21181 ctggacaggc ttgaatactg aagtttatat cttcccgacc tttaagcaaa tgaagacata
    21241 cgatgagccg ttcagcttcc agatgagcga cggaacaacc attggttacc acatcggcgt
    21301 agcctacaag gttgatccag caaaagtcac aacggtattt cagacctatc gcaaaggtgt
    21361 agatgatatt actgataccg atctacgcca gaaggttgca gatgctctga accggttagc
    21421 cagcaaaatg accaccgaca aatttatcga cggtggcaaa tctgaattgc ttgatgcagc
    21481 ccttaaagac attcaggaag aaatgacgcc aatcggcatt caggtgatga gcctctctta
    21541 tgtaggtaag ccagaatacc cgccaacagt tattgacagt attaacgcca aagtcacggc
    21601 aaaccaaaaa accctgcaac gcgagcaaga ggtcaaacaa cgtgaagcag aggccaacat
    21661 gctgcgcgcg gaagctgccg gacaggctga tgctattcgc acaaaagccc aggccgaagc
    21721 tgatgccatt cgtttacgtg gtgaagctct gcgccagaac cccggtgtta tggagttgga
    21781 agcgatcaac aaatggaacg gtacattacc gcagtatatg accagtaata ccgctgttcc
    21841 gtttgttccg gtgaaataaa agcgtaagca aaattggcag taatccggcc ctttagctca
    21901 gtggttagag ctggcgactc ataatcgcac ggtcaccggt tcaagtccgg taggggccac
    21961 catatttggt tgtaacacgg cgtctggcac atgcgtcgtt agcggtctgg tgacgttaaa
    22021 aggggggaac cttgccccta gctcaggcaa cgaaccaggt agccggaatg tgcaagccac
    22081 cgtttgttgt ttctcgggta aagggattca ccatcctggc gattcggtgt gacagccggg
    22141 aagagtccgg cgcattaatc ctgattttct ggtgatgact catatcgtta ggagtgattt
    22201 gagtatgccg attatatctg acattcagca cgcctgggtg gagtgctaat gtctgcatcc
    22261 cctcttgaat ccatgccaaa ttcccttagt gcagaacaag ctgtacttgg tggcttaatg
    22321 cttgataact gccgctggga tgaagttgca gatcgtatag ttgctgatga tttttatacc
    22381 agtgctcatc gtgaaatttt cagtgagatg gagaggttat taagtcatgg caaaccgatt
    22441 gatttgataa cacttgctga agcacttgaa cagaacggta aattagaacg cgccggtggt
    22501 tttgcgtacc ttgcggagat gtcaaagaac acgcccagcg cggcaaatat ttgtgcttat
    22561 gcggatatcg ttcgtgaacg cgcggttgtt cgtgaaatga tttccgtcgc aaatgaaata
    22621 gccgaagctg gatatgcgca ggatggcagg ggcagcaatg aattgctaga tatggccgag
    22681 cgccgcgttt ttgaaatagc tgaaaaacga caaaagagcg gtagtggtcc aaaagatatc
    22741 gccagcattc tcgatgcaac ggtatctcgc atagaagagt tgtttcagcg accacatgat
    22801 ggtgtaacgg ggcttgatac gggatttacc gatctcaata agaagacggc agggcttcag
    22861 ccgtccgatc tcatcattgt cgccgcccgc ccatctatgg ggaagaccac gtttgcgatg
    22921 aatctcgtcg aaaatgccgc agttcgtaac gataagcccg tattggtttt tagccttgag
    22981 atgccgagcc accagctgat gatgcgctca ctggcttctc ttgcacgcgt tgatcagact
    23041 cgtattcgga cagggcaact taacgacgag gattgggcgc gggtttctgg cgcaatgggt
    23101 attctgttgg acaagcagaa tatttttatt gatgactcaa gcgccctgac acctacagag
    23161 cttcgttccc gcgctcgtcg tgtttataaa gaaaatggtg gtttgagcat gattatgatc
    23221 gactacctgc aacttatgcg cgtccccgag ctgcaagata accgaacgct ggaaattgcc
    23281 gagatttctc gctcactgaa ggcgttggcg aaggaattac aagtaccggt ggtggcattg
    23341 tcacaactta atcgatcgct tgaacagcgt gcggacaaac gaccggtaaa ttcagattta
    23401 cgtgaatcag gagcaattga gcaggacgca gacctgatca tgtttctgta tcgcgacgaa
    23461 gtttatcacc cggatagcga aatgaagggc attgccgagg taattatcgg aaagcaacga
    23521 aatggcccaa ttggcacggt gagattggct tttaacggcc aatactcacg gtttgataac
    23581 tatgctggtg ctgactggca agaggattat taatgcaatg gaatgaggaa aagccgatga
    23641 acatcctgat cattgggcga aaatttgaag ctatcagtga tgtgaaaaca tatacggaaa
    23701 tgtgggctta taacctggcc tgcgccttta gtgaggcagg ggtaacattg caataccatc
    23761 gtccatattc ccctggcgtc gaaagcccgg aggattatgt tgaagctgtg ttgactgctg
    23821 cgaccgcatg ttctgcgaag gccattttgg caccaggatt gaggtatttt actacggtac
    23881 ccagggaaat aggcatgcaa ctgtgtcgcc gattctctgg atgggtagcc caggtatatg
    23941 acggttctat gctggattcg gcaccagtcg atattacttt tactgtccgc gatgatacct
    24001 ggcggtacct ggataatccc ggcaggttag agcgtcataa tcgttttaac aaacatgttg
    24061 gatgggcagc gaatcaggag ctgttccatc tggaaaccaa aacagacgat attctgcgta
    24121 tttttgtaga ccacgctgca tttgatgtta gtggttttga tcactcctta agtatcctta
    24181 tgaaccttca gcgtctgacc gttccgtatg aggccagaac gttgactgat gacggattgg
    24241 ttaccattga tccggggaat atttcggtaa ctccatacag gcggacgccg gtgccagcaa
    24301 ccgaatttgc agctgaattg cgtaagagtg acgtttttat cgttacgcat cccgaaagcc
    24361 ttggattaac tgttcttgag gcggcaatgt gtggagcgtt gatattaact ccgcccgatt
    24421 gccttccgcc ggatcgcctg gagttggtga accatatggt tatcaagtcg cggattgatt
    24481 gggatgaggt tattgctcgc gttgatcgcg tgaaaaatgc tgaaaaggtc cagtgtcaca
    24541 cctggtcggc aattgcggaa aagatgcttg agacgtttat cacgcagaaa ccgtcgcgcg
    24601 gtaacggata aaaaattgaa cccgtcataa cagaaaagcc cgatcgccgg gcttttctta
    24661 agccttgtca acagagactt gagcggcttt tatggataga ttcccgctgg cctctatcgc
    24721 catacttccc cccgccttca gggcgacatc cgcgcctgac tttatatcga gatttcctgc
    24781 ggaagagatg aatgccggac cttgagaaat ggcatataac tccccggcct cgttgaaccc
    24841 gattgttgtt ccacttttca agtgcgtaac ggcccaggct ccgcccgccg tccggatctc
    24901 cattagtccg ttccgcgacg aaataaagtc ttttttggcg ctggttgatg gttgtgctgg
    24961 tgcaccttca acttcaggcg gtacatagcc ttcaccttgt cctgacgctt caggcggcac
    25021 attgggagcg ccaccggatg catcctgtgc ataaccgatt atcaatggcc atcgcgaatc
    25081 cccattgtag ggaaattcta cccatacttt atcgccgggc agaaatggtg aaaacgtgtt
    25141 tgcattggac aatatagctt ctgcccacgg caatgaggca tctggtaacc catccatcat
    25201 gccgacaaca cgtatttgtg tacgcatcag acctttaggg tcatcgacgc ttatcactac
    25261 agcccgatac ttccctgtca aactacccat tcaccactcc taactgtgca cggctgacaa
    25321 aacgaaagcg gtcttcgaaa tgagtcacgg acatcactat cattttgtca gggattgatt
    25381 catcgagttc tccgtcacct gccgtgttat gcacgacaat tttcagcgtc gtacccggag
    25441 ttagcgcggc atttccttcc accagcatat cgaggcgggg gagaatgaat ttgttgtagt
    25501 tcgccagcgc ggtaggatcg ggattgctcg taaatttaat ggggtcttcc tggttacctg
    25561 agtaaaccac acctttggtc atgtcataac tggccattct gtaattgtgg cggcgctggt
    25621 attcataatc ggcattcagg atgttgaact gactaattgt aaatccggat gtgttgggat
    25681 tggcggactc ataagtaagc gatggagcgg cgtttgccat tttttccata cttttaaaat
    25741 tgatcgtccc cctggatgcc cagcacatag aaccggtatc ccgggctatc tcctgcaata
    25801 ccttggtcgg tttttctcca acatttaggt ggtatgtgga tgtttttctg aatgagtcag
    25861 catttacctt cagaccaggg gcaagagagg aaactacggc tgatgggggc ttatcaacaa
    25921 aatactgtgc gctggtggac ggaactttta ataaccgcac cgggttacta aacgcgtaaa
    25981 tcagtacagt atcgtccttg cgcggcgctt taagaacaaa gaactcttcc gagaagagga
    26041 tgccgccatg accttccgga tcaccaagtg aaacggtcag tattgtccca aatttcaccc
    26101 ccagcttatt gaccacgtaa gccgttgaat ccctgaccat gagcataagc tggggaccag
    26161 atagctcccc gggttcgaca taggtacatc ctacgatcat ttcgcgaggg atttcgttct
    26221 gcccaattga aacagattgc aggaatagct gagtgcgttt tgaatcagtt tccggggctg
    26281 tggtggtctt tgtggccatc tcattcctcc agaattttcg cttttaccgt tatggtgccg
    26341 gtggtttgct gcatataagc caggatagga agctccgcca ctactgtgag gttcaatcca
    26401 accgcgaaca gcctgttgtc ggcggtgccg gtggtcagat cctgaaatgc gattgatttt
    26461 tgcccttcta tgtaacaggt aaccggtatc tcataaccgc cgacattggc agtgtgagtg
    26521 aaagatgcct gcccgaggct ggcatacatt cgtagccaga atgctaatgc agttgtaacc
    26581 atcccaagag attccttctc gtcactggct atccatagcg aatattccag tgagaaaggg
    26641 atagtcgata ccagggcttc aatctcatca ttttcattgg tgacatgccc ttcatcgtaa
    26701 ttatcccggc acagttcacc ttcataaatt gaaaatgcgg gagaacgaga cagattcaca
    26761 agcggcattg ccagcttatt taccgggcca gcagaggctg tatctttgcg cccggcgcga
    26821 tcggcttcaa atgacgacaa ccactccttc acatcactaa aagtgccgag cgttatgcga
    26881 tctcttggtg tgcgtttcag gaactccctg aatgactggt tagtgcgatc attaaagctg
    26941 acaacttgtg agtcgaatgc ttcgtttaaa gcctgtgcga gcgccgaatc aatgccatca
    27001 atagtggcaa attccagctt accggttgga gtaagacctt ttttcttaaa gatggccagt
    27061 agccattcct gattattcag aatcaccgat gaaattccct tcaaaggcgc gtgaaggcac
    27121 gcaataaaac aaactgccta ccctggcagt gccgtaattg aatattttat ggatgtacca
    27181 gaagcggcga atggttgtgc cgtctgacag ctgttccagc cattcgagca tagaacccac
    27241 tggcacatta acggcggcca accgaaggat taaagcactg tcgctaattc ccgtattatc
    27301 actgccgtcg tatagcgcgt agaaggcgtc catctcatcc gggcagtcga gggctgttat
    27361 cagttctgga tcctgatagt catatatgcg ttggttcggt tctattattt cagatgccgt
    27421 ttcaggtgca tttttgtctc tgtaaggtat tgcgcgatac agaaccgcat cgaatgagtc
    27481 agggtctagc ttgattgctt tgagccagtc catccgcaca aggttattaa aaactgcatg
    27541 accttgataa cggtggcgca caccagaatc actaagcagg ccgtgatcca gattgggaag
    27601 gtgattgtcc tccacaggat caacaatatt accaacgtta acaccatcgg tttcgatttc
    27661 agcatcaata tcttcctctt caatcagttc agaaccttcg cctggaatat ccggatccga
    27721 ttcggtgtcc gggaggttat caccagtcac ttgttgtgat ggttctgtgt cctcaaacat
    27781 gtcatcaaag aaaccagcca tcgattatcc tttccgttta cgggcttcgt taatttgtgt
    27841 ctcaagaatg cttcgcgcct gcgcagtggc agcggccttg tccattccct gactcatgaa
    27901 aaactttatg aggttgttcg cctgcgtttg cagggctttt ttgagagcgt cggcttcagc
    27961 gcgagcctgg gcttccctca cccgcgatgc ttttagttcg gcattcttcc tgtttgccgt
    28021 ggtgcgagct ttttttaaca accggcgaac gttgtccgtg gcgctatctt tggcgcgtag
    28081 ttttttgcct aatgcatcct gagatttcag atataactca tactcacgcg ccgctttagc
    28141 ctgatccgtc gttgttgtcc ggttgcgcgc gagcgattta gccagttcgc ctttgaaata
    28201 ggttgttgtc ttccgcttgt catcgccgaa ggctacctgt tcagctgctt tttccagggc
    28261 aataatgatg gccttgtgcc atgtgggaga ctgaaaacgc gtcatagcgt gcaacacatg
    28321 tttgcaggct acaccagtca gatcagggtt gcggatcttg gggaatgcat actcttttgg
    28381 cggcgcgaca gcatagttac cagcagtggc catataacga taccagtatt gatggcgtcc
    28441 acaatcacag tcgaaagata cccggccctt gcagagatcg gcagcgattc gggctttttt
    28501 cgcaccgtct tcagcaattt cctcaacggc tttatcccat tcctcaaatc gaattctgac
    28561 acggtgatgc tggtggaccg actcatccga ggcattaaca gatatcaatg caaggttgtg
    28621 ttttagcccg aggaatgtcg cggctttgat ccctgtgcca tcagaaacct tgttgttagc
    28681 gcgttttata tcaatgctgg tggactgcgc caccagctga gcataggtaa tgccgggtac
    28741 cgtgctcttg aatttggttt tatgagcctg ccttgaggtg ttgaaactgc gtatatcttc
    28801 gggcgtaaag taggtgccat ctttcttttt cccaaggctg aggaatgcct caagttcgcg
    28861 gttacgcatc cccataatcc ttggggtgag tgtacgtcgc gcgtttcgcc gattctgacg
    28921 ctgctgttta cggataagat cgaagacctt gttaaagtct tttgcactta atccatcagt
    28981 ctgatagcga ccaaggttgt cgcgagcata ttcagttggc attcatttcc cttacgcaat
    29041 ggataatgtc cctattacct ggccgtcgta ttggaaatgg cgaatcattt cgcggatcca
    29101 ggtggcaggt gggagtttta attttttgcc aacagtcata ccctgagact catcctcaag
    29161 cccggcggcg agcgtcacaa cccagcgtag ctctgctatg ccccacatac ggtaagccag
    29221 caaatccggg cgatattgct catcgggaag aacgtaataa atcgtcagat tcttgtcgtt
    29281 cgattcacac ataagcatca cctctttgcg tagctctgcc ctgagtattg gatcggctat
    29341 gttgcggtcg tcataccgcg acagaggata ttgccgggtg ctttgggttg tagtgattga
    29401 tgtagccata gtcagcctgc cagaaataga tgatggtgat tctaccgcta gtcatttgtt
    29461 gaatatttaa ctcaataaaa gaaaattatt agtgcaattc tgattgtgaa atgtatcatt
    29521 ctgcccttaa gtaggttctt cacgaggaaa caaaattggc agaacgtgtt gatgatgcag
    29581 agctgagcat gaatcagtta gaagctctca aagacatggc catcgataac atcagaaagc
    29641 aggcacaggt cgtgagccag gtatttacag ggaagtgtcg ttactgcaat gaatcgattg
    29701 aatcaggcat ttattgtgac gctgaatgtg cgcaatggca cagggaagag caggccgcaa
    29761 aacagcgtaa atatggcatg cgaccggcag gatttgactg attatgttgc gctttactga
    29821 ggaagagttt caggctttta gtgagcgtcg aaataagggg cggtccaggc caaaaaccaa
    29881 aaaggatcca ttcttatcgc ttgcgccggt aaaagaagtt tctccacatg cgaaggcact
    29941 tgcagcactt gcaaagaacc cagacctgcg cgacggaaat tgcgagcact tcgagcaggt
    30001 tttcattttt gattacttcg aacgcaagca ccctgacatc tatgagctgt tgcatgcaac
    30061 gcctaacgga gggaaacgtt caaaagcaac cgccgggaaa atgaaggctg aagggcagaa
    30121 aaaaggttat ccggacatga gtctcgataa agcatgcggt atttatcacg gcatgcgaat
    30181 tgagcttaaa gaaccaaatg gtaaagcccc gacgaaagag cagatcgcct ggatgcgcag
    30241 gcttagagag gaaggctact acgttgttct tgcgtatggt gcagaacaag cgattaccgc
    30301 catcctggaa tacataagtc ttaaaaaggg tgaggctatt gagcatgtat tgaacggcga
    30361 taagtggttg catgctgctt aaaataataa attaattagt gcatgtacgc tctttgtggt
    30421 agtgcacttt aacatcggga gaataatcgt gtcatccaag gttaattatg agtcgctggc
    30481 atcggtcatg ccgcgtaatg aacaggaaac agatgctgta gtggaccctg taatcgctga
    30541 aatgaatgct cgcctggagg ctgaatttgc agctgagaat gaacatacca cccagggcga
    30601 ctaggactgt tttttgtgtc ggtagcggtc cgtcactcac tcgtgaggac tgtgctgcta
    30661 tagaaaaaac tggctgttca atcatcgcgg ttaacaattc ctggcagatg ttcgatgaca
    30721 tttatgcttt atacgccggt gatttgtcat ggtggaagca atacggatcc accataccgg
    30781 gagggagatt ccgcaaagtg acagccaacc tggcggcggc gaaatcattt tcgttggagt
    30841 acaggcgata ttgtggaccg gcggaagggg taaatagcgg cgcgcaggct atcagtctgg
    30901 ctgctgaatc aggggctgaa gtagtggtat tagtcggcta tgactgttct ctgcaaaacg
    30961 gccttcattg gcatggcgcg caccctcaag cactacggaa tccaacgcag gtgtctattt
    31021 caaaatggca acagcagttc ctggataccc gcaaaaaaca cgcagattta catattttga
    31081 atgcaagtag gagcagtgca attcaatgtt tcccaagaat aaatttagag gcagtgatcg
    31141 cgttattatc gtcggcagtg gcccaagcgc cgcaaacttt gttgcgccgc gcggagtgcc
    31201 gattatagcg gtcaatgggg ccatcgactg gcttaaccgc gcttcttatt ttttcaccct
    31261 tgatccatcc ccagacaata tgcggcgcgt tggtcgtggc cgccgtcgcc gtggtgtttg
    31321 ttattgcatg gcactacccg atgttaaaga acgtgaagtc agagatggcg ttctgtgctt
    31381 ccgtcgtgtg gctgaacgcg gcatggagcc aaaaaatacg aattctcccg agtggtgggc
    31441 gtggcgctgg tccgcacatt tcggcctttg cgaagatgag aatgaaattg ccagcggcaa
    31501 tagtgcatat ggtgctctga acctggcttt ccatatcgga ttcaaacatg tagctctggt
    31561 gggcgttgac gctacgcaag aactacgcgt tcactccggc ggcacgccaa aaaatctaag
    31621 tcacctgcct ttgttattcc agtctgcgcg tgaacagatt gacgttgttt catgcgggaa
    31681 aatgggaggt attccgcaga tgactcttaa agaatggctg aagaatacat gatggcaccc
    31741 acaatttatc accgtatcga cggtaccaaa tacaggaatg tctgggttgt tggtgatctg
    31801 catggttgct acaccagact gatgtccgaa ctccatcgtg tggattttga cccggcgcag
    31861 gatttactga tatcggtcgg cgaccttatc gatcgcggta ctgaaaatgt cgaatgtctg
    31921 gaactattgc agatgccctg gttcagggca gtgatgggga accatgagcg gctgatgatt
    31981 gatgcgttaa gtccagatgg caacgtgaat aactggctaa tgaatggcgg acaatggttc
    32041 ttcatgctgg acactgatca ggaaatatta gcctgggcgc tggtggagct ggtaaagcgt
    32101 ctgccctata tcattgagtt gaacacgggg caagaaacta tcgttatagc ccatgccgac
    32161 tatccggata atgaatacca attcggtaag gaggtgccgc ttttcaacgt tgtctgggcg
    32221 cgcgagcgta tcagtgattc gatggatgat attggtggcg aaatttcggg cgcagatcgt
    32281 tttatctttg gtcacactcc ggtgaaaagc ccgaagacat tctggaatca gcagtatatc
    32341 gacactggtg ccgtattttg cggaaacctg acattgatga aagtgaaagg tgatggtgca
    32401 gcatgaagat tgctttagtt tttcgctctg gtggtgacta taacgcttcc gatgtgcagt
    32461 ggctggttaa tcaactgcca aaaggctatg aaattatttg cctgacagac ctgaagcgtt
    32521 tacatgtacc tggcgtcaaa gttgtcccat tgatcaacca gtggcaaaag tgccgtggct
    32581 ggtgggcgaa aatcgagttg ttccgaccgg atataaccga tgatctgttc tatctggatt
    32641 tggacacggt tattgccggt gatatacgcc caatcctaga gcatccacca accagcttca
    32701 ccatgcttag ggatttttac catccacaat atcgtggcag cggtgccctg tggataccaa
    32761 atagtgttaa agcgcatatc tggagttcat tctggcaaga tccggaaggt tggatttctc
    32821 gttgtgtcac tactgagtgc tggggtgacc aggggttctt acgaaaggtt atgggcgatg
    32881 atacaccagc atttcaggat ctgtatccag gatggtttgt aagttacaag gccgatgttg
    32941 tggaacctgg ttcaaaatat gcgagcgcgc gttactccag ggggaatggg gcattaccaa
    33001 aagactgccg aataatcttt ttccacggca aaccgcgacc tcgcgaagtg tcagaggatt
    33061 ggcttcccct tatcagctca ttttttgagc gagaatcaga ataatattgc tctaataatt
    33121 ccata
//
LOCUS       DAPGOF010000038        32760 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.038, whole genome shotgun sequence.
ACCESSION   DAPGOF010000038 DAPGOF010000000
VERSION     DAPGOF010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.038"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(94..3243)
                     /locus_tag="RHV13_001544"
     CDS             complement(94..3243)
                     /locus_tag="RHV13_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HDV1241094.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(3266..4459)
                     /gene="acrA"
                     /locus_tag="RHV13_001545"
     CDS             complement(3266..4459)
                     /gene="acrA"
                     /locus_tag="RHV13_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HDV1241095.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4601..5248
                     /gene="acrR"
                     /locus_tag="RHV13_001546"
     CDS             4601..5248
                     /gene="acrR"
                     /locus_tag="RHV13_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HDV1241096.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            5376..8738
                     /gene="mscK"
                     /locus_tag="RHV13_001547"
     CDS             5376..8738
                     /gene="mscK"
                     /locus_tag="RHV13_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HDV1241097.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRVKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLSDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(8950..9111)
                     /gene="rsmS"
                     /locus_tag="RHV13_001548"
     CDS             complement(8950..9111)
                     /gene="rsmS"
                     /locus_tag="RHV13_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HDV1241098.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDHEAEK"
     gene            complement(9125..9652)
                     /gene="priC"
                     /locus_tag="RHV13_001549"
     CDS             complement(9125..9652)
                     /gene="priC"
                     /locus_tag="RHV13_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HDV1241099.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFKTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            9722..10099
                     /locus_tag="RHV13_001550"
     CDS             9722..10099
                     /locus_tag="RHV13_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HDV1241100.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10252..10803
                     /gene="apt"
                     /locus_tag="RHV13_001551"
     CDS             10252..10803
                     /gene="apt"
                     /locus_tag="RHV13_001551"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDV1241101.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10932..12863
                     /gene="dnaX"
                     /locus_tag="RHV13_001552"
     CDS             10932..12863
                     /gene="dnaX"
                     /locus_tag="RHV13_001552"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDV1241102.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAAQQAPTVPLSETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFGAELDEESIRPI"
     misc_feature    12196..12260
                     /gene="dnaX"
                     /locus_tag="RHV13_001552"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            12916..13245
                     /locus_tag="RHV13_001553"
     CDS             12916..13245
                     /locus_tag="RHV13_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDV1241103.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13245..13850
                     /gene="recR"
                     /locus_tag="RHV13_001554"
     CDS             13245..13850
                     /gene="recR"
                     /locus_tag="RHV13_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDV1241104.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            13960..15834
                     /gene="htpG"
                     /locus_tag="RHV13_001555"
     CDS             13960..15834
                     /gene="htpG"
                     /locus_tag="RHV13_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HDV1241105.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            16015..16659
                     /gene="adk"
                     /locus_tag="RHV13_001556"
     CDS             16015..16659
                     /gene="adk"
                     /locus_tag="RHV13_001556"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDV1241106.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            16791..17753
                     /gene="hemH"
                     /locus_tag="RHV13_001557"
     CDS             16791..17753
                     /gene="hemH"
                     /locus_tag="RHV13_001557"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HDV1241107.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(17750..18709)
                     /gene="aes"
                     /locus_tag="RHV13_001558"
     CDS             complement(17750..18709)
                     /gene="aes"
                     /locus_tag="RHV13_001558"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HDV1241108.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPPWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADETLRDGAQFFTAQL"
     gene            18861..20165
                     /gene="gsk"
                     /locus_tag="RHV13_001559"
     CDS             18861..20165
                     /gene="gsk"
                     /locus_tag="RHV13_001559"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HDV1241109.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(20298..21974)
                     /locus_tag="RHV13_001560"
     CDS             complement(20298..21974)
                     /locus_tag="RHV13_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HDV1241110.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALDLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(22215..23435)
                     /gene="fsr"
                     /locus_tag="RHV13_001561"
     CDS             complement(22215..23435)
                     /gene="fsr"
                     /locus_tag="RHV13_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HDV1241111.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDSRHKD"
     gene            23653..25305
                     /gene="ushA"
                     /locus_tag="RHV13_001562"
     CDS             23653..25305
                     /gene="ushA"
                     /locus_tag="RHV13_001562"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HDV1241112.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYNNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(25342..25821)
                     /gene="ybaK"
                     /locus_tag="RHV13_001563"
     CDS             complement(25342..25821)
                     /gene="ybaK"
                     /locus_tag="RHV13_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HDV1241113.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(26025..26819)
                     /locus_tag="RHV13_001564"
     CDS             complement(26025..26819)
                     /locus_tag="RHV13_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HDV1241114.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            26957..27298
                     /locus_tag="RHV13_001565"
     CDS             26957..27298
                     /locus_tag="RHV13_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HDV1241115.1"
                     /translation="MKQATRKPTTPGDILLYEYPEQIALKINELVELLHVHRNSVSTL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(27715..30219)
                     /gene="copA"
                     /locus_tag="RHV13_001566"
     CDS             complement(27715..30219)
                     /gene="copA"
                     /locus_tag="RHV13_001566"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HDV1241116.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALI
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADFDEAQALRLAAALEQGSSHPLAR
                     AILDKASDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            30481..31413
                     /gene="glsA"
                     /locus_tag="RHV13_001567"
     CDS             30481..31413
                     /gene="glsA"
                     /locus_tag="RHV13_001567"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HDV1241117.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGKVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVTSVAKQLGYNVFKG"
     gene            31416..32708
                     /locus_tag="RHV13_001568"
     CDS             31416..32708
                     /locus_tag="RHV13_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDV1241118.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
BASE COUNT         7915 a         8676 c         8624 g         7545 t
ORIGIN      
        1 gagatcctga gttggtggtt caattactcc ttaatgttcg taggttatgc ataaaaaagg
       61 ccgcttgcgc ggccttagtg attacacgtt gtatcaatga tgatcgacag tatggctgtg
      121 ctcgatatct tcattcttgc ggctaaagcg gcggcgaacc accacaaaga ataccggaac
      181 gaagaagatt gccagtaccg ttgcggtcac catcccgccc attacaccgg tacctactgc
      241 gttctgcgcg ccggaaccag caccagtact gataaccagc ggcataacgc cgaggataaa
      301 cgccagcgag gtcatcagga tcggacgtaa acgcatccgc actgcatcaa gcgtcgcttc
      361 aatcagacct ttaccttctt tatccatcaa gtctttggcg aattcgacga taagtatcgc
      421 gttcttcgcc gacaacccaa tggttgtgag caggcctacc tggaagtaaa cgtcattggt
      481 caggccacgg aaggtggcag ccagcaacgc accgataacc cccagcggaa cgaccagcat
      541 aacggagaac ggaatcgacc agctctcgta cagcgccgcc agacacagga atacgacaat
      601 caacgaaatc gcgtacagtg aaggtgcctg gttgccggag aggcgttcct gataggacat
      661 ccccgtccag tcatagccaa caccggtagg cagtttgctc gccagttgtt ccattagctc
      721 cattgcttca ccggtacttt tacccggtgc cgcctggcct aagatttcca tggatggcag
      781 gccattgtaa cgttccagac gcggcgaacc gtactcccaa cgagaagagg agaatgccga
      841 gaacggcacc atctgaccat cagcagcacg aacataccag tcgccgatat catccggcag
      901 catacggtat ttcgcttctg acatgacgta aactttcttc acacgaccgc ggtcgataaa
      961 gtcgttcaca tagctaccgc cccatgcagc gcccagagtg gtgttaatgt cgttgataga
     1021 aacacccagc gcctgcgctt tttcctggtc gatatcaatc ttaaactgcg gggtatcttc
     1081 cagaccgttt ggacgtacgc tggtcaacat atcagggtgc ttcgctgctt ctgcaagcag
     1141 ctggttacgc gcctgagtca gtttttcgtg accaaggcca gcctggtcaa tcagctcaaa
     1201 gtcaaagccg gttgcagtac ccagttccac gattgcgggc aggttaaagg cgaaaaccat
     1261 cgcatctttg atttgcgaga aagcgcgtgt tgcacgcatg gtaatcgctt caactttgtt
     1321 ttcttcaccc ggacgatcgg cccagtcctt caaggaaacg aacgcaatac cggtattctg
     1381 accacgtccc gcaaagccga agccgttaac ggcgaacacc gactcaacgt tgttcttttc
     1441 tttggtcaga tagtaatgcg ttacctcatt gagcactttc tgtgtacgtt cctgcgttgc
     1501 acctgctggc agctgaacca tggtcataaa tacgccctgg tcttcatctg gcaagaagga
     1561 gcttggcaga cgcacgaaca gataggccat gccgaccacg atgatcagat acagcaccag
     1621 gtaacgcccc gtactgcgca gaataccgcc tacgctgtcg gtgtagtggt gcgtgctctt
     1681 ctcgaacatg cggttaaacc agccgaagaa gcctttttta ccttccccgt gatcgccttt
     1741 ggcaatcggt ttcagcatgg tggcacaaag agctggagtc aggatcaacg ccaccagtac
     1801 cgacagcgcc attgctgaaa caatggtaat agagaactga cgatagatag caccagtaga
     1861 accgccaaag aaggccatcg gtacgaatac cgccgacagt accatcgcga taccgaccag
     1921 agcgccctga atctgcccca tcgacttacg ggtggcttct tttggcggca aaccttcttc
     1981 cgccatcaca cgctcaacgt tttctaccac aacgatagcg tcatccacca acaggccgat
     2041 ggcgagcacc atcccgaaca ttgttagcgt gtttatcgag aagccaaagg cggcaaggac
     2101 ggcaaaggtc cccagcaata ccaccggtac ggcaatggtc ggaatcaacg tcgcgcggaa
     2161 gttctgcagg aacagataca taaccaggaa cacgaggatg atcgcttcga ccagcgtttt
     2221 aaccacttcg tgaatagaga ttttcacgaa cggcgtggtg tcgtacgggt aaacaatttt
     2281 cagacccgac gggaagaacg gttccatctt cgccagttca gcacggattg ccgcagcggt
     2341 atccagcgcg tttgcaccgg tcgccagctt gatccccaga ccggaagccg gttggccgtt
     2401 aaactctgcg atgatgtcgt agttctcacc acccagctca atcttcgcca catcacgcag
     2461 cagcacgcgg gaaccatcct gattcacttt cagcaggatt ttgccaaact cttcagtaga
     2521 ggtcagacgc gtctgagcaa taatagaggc gttaagctgt tggcctttca ccggcggcgt
     2581 accaccgagc tgacccgccg caacctgggc gttctgcgct ttgatggcgg taatgacatc
     2641 aaccggcgtt agctggaatt tgttcagctc attcgggttc atccagatac gcatcgcgta
     2701 ctgtgaaccg aacaactgaa catcacccac gcccgacgta cggctgatgg catctttcat
     2761 attcgccgct acgtagtcag agatatcctc ctgcgtcatg gtgccatcgg tgttgataac
     2821 gccgacaacc atcaggaagc tgctggatga tttctcaacg ctcacccctt gctgctgaac
     2881 ttcttgcggc agcaacggca tcgccagctg cagtttgttc tgcacctgaa cctgcgcgat
     2941 atccgcatca gtaccagact caaaggtcag ggtgatctgc acggtacccg tggagtcact
     3001 gttagaggac atgtacatca ggttatcgat accgttcata ttctgttcga taacctgtgt
     3061 caccgtgtcc tgcactgttt tcgcatcagc gccggggtag gaggcggaga tcgttactgc
     3121 cggcggtgca atcgtaggat attgcgccac cggcagtttg aggatcgcca gcccccctgc
     3181 caacatgatg ataatggcga tcacccacgc aaaaatcggg cgatcgataa agaaattagg
     3241 catgtcttaa cggctcctgt ttaagttaag acttggactg ttcaggctga gcaccgcttg
     3301 cggcttgctg gttattatca gcggtaactt cttgtgcttt tacctggaca ccaggacgca
     3361 ctttctgcag cccacttatt actacgcgat cgcctgcttt cagaccttct gtcaccagcc
     3421 acttatcgcc aatagcctgg cttgcaacga tcggacgggt ttccactttg tcatccgcgc
     3481 caactaccag tacggtggca tcgccacgcg gcgtacgggt tacgccctgt tgcgggacta
     3541 aaatagcgtt tggattaagc ccttcttcca gacgtgcgcg cacgaacata cccggcagca
     3601 gagtgtgatc cgggttcggg aagatagcgc gtagggtgat agacccagtg gtctgatcaa
     3661 cggtaacgtc agagaattcc agcgtaccgt cctgcgggaa cttaatgccg tcactggtga
     3721 tcagcgacac tttggctttg ccgttctctt gtttcagcgt gccattcgcc agttcctgtt
     3781 tcaggcgcag gaagtcgttg ctggactggg tcacatcaac gtagatcgga tcaagttgct
     3841 gcacggttgc cagcgcagtc gcctgaccgt tctgtaccaa tgcgccttcc gtcacgttcg
     3901 acttaccaat gcgaccgcta atcggagagg tgactttggt gtaagccaga ttgatccgcg
     3961 cagtttcaac ggcagctttc gccgcagtta ccgcagcatt cgcctgttgc gcatcagcca
     4021 gagcctgatc gtactcttgc ttactgatgt actgagtacc gagcagtttc tgataacgat
     4081 tcaccgtcaa ttgcgcgata ttggctgcag cctgggcttt cgccagatca cctttcgcac
     4141 tgtcgtatgt cgcctgatag gtcgcaggat caatctgata gagagagaca cctgcttcga
     4201 tgtcgctacc ttctttgaaa ttacgcttca ggataatccc gctaacttga ggacgaactt
     4261 ctgcgatccg gtaggcactg gtgcgacccg gaagctcggt tgtgatctgc agaggttcag
     4321 ttttgactgt tactacgcca acggcgggca tctgctggcc accttgttgg gcctgtttgt
     4381 cgtcacatcc tgttagggct aagctgcctg agagcatcag aacgaccgcc agaggcgtaa
     4441 accctctgtt tttgttcata tgtaaacctc gagtgtccga tttcaaattg gtcaatggtc
     4501 aaaagttaat aaacccattg ctgcgtttat attatcgtcg tgctatggta catacattca
     4561 caaatgtatg taaatctaac gcctgtaaat tcacgaacat atggcacgaa aaaccaaaca
     4621 agaagcgcaa gaaacgcgcc aacacatcct cgatgtggct ctacgtcttt tctcacagca
     4681 gggggtatca tccacctcgc tgggcgagat tgcaaaagca gctggcgtta cgcgcggtgc
     4741 aatctactgg cattttaaag acaagtcgga tttgttcagt gagatctggg aactgtcaga
     4801 atccaatatt ggtgaactag agcttgagta tcaggcaaaa ttccctggcg atccactctc
     4861 agtattaaga gagatattaa ttcatgttct tgaatccacg gtgacagaag aacggcgtcg
     4921 attattgatg gagattatat tccacaaatg cgaatttgtc ggagaaatgg ctgttgtgca
     4981 acaggcacaa cgtaatctct gtctggaaag ttatgaccgt atagaacaaa cgttaaaaca
     5041 ttgtattgaa gcgaaaatgt tgcctgcgga tttaatgacg cgtcgcgcag caattattat
     5101 gcgcggctat atttccggcc tgatggaaaa ctggctcttt gccccgcaat cttttgatct
     5161 taaaaaagaa gcccgcgatt acgttgccat cttactggag atgtatctcc tgtgccccac
     5221 gcttcgtaat cctgccacta acgaataacc ctgaatctga ctccaggatt tttcctggac
     5281 attttcgtcg ttgctattct ggttcactgc gtcgtgatat tcttgcggtt tgactttttc
     5341 aggtcgttct tcaggttcag aaaccttcat tcatcatgac tatgttccag tattacaaac
     5401 gatcacggca ttttgttttt tcagcattta ttgcttttgt ttttgtcttg ttatgccaga
     5461 acacggcgtt tgcgcgggcg tcatcgaatg gtgatctgcc gacaaaagcg gacctgcagg
     5521 cgcaacttga ctcactaaat aaacaaaaag atctttctgc tcaggataaa ctggtgcagc
     5581 aggatctgac agatacatta gccaccctcg ataaaatcga tcgcgtaaaa gaagagaccg
     5641 ttcagctacg gcaaaaagtc gctgaagcgc cggaaaaaat gcgccaggcg accgcggcgt
     5701 taacagcact tagcgatgtc gataacgacg aagaaacgcg caaaattctg agcacgcttt
     5761 cgttgcgcca gctggaaact cgcgttgccc aggcgctgga cgatttgcaa aacgcacaaa
     5821 acgatctggc gtcttataac agccagctgg tttcgttaca gacgcagccc gaacgcgtgc
     5881 aaaatgcgat gtataacgct tcgcagcagc tgcaacaaat tcgcagtcgt ctggatggga
     5941 ctgatgtcgg cgagactgcc ttacgtccca gccagaaagt gttaatgcag gctcagcagg
     6001 cgttgctgaa tgcggagatt gaccagcagc gtaaaagcct ggaagggaat accgtcttgc
     6061 aggatacctt gcaaaagcaa cgtgattacg tgacggcgaa cagcgcccgt ctggagcacc
     6121 agttacaact gttgcaagaa gcggtaaaca gcaagcgcct gactttaacc gaaaaaacgg
     6181 cgcaggaagc cgtctccccg gatgaagccg cacgtattca ggctaatccg ctggtgaagc
     6241 aggaactgga aattaaccag cagttaagtc agcgtctgat taccgcgact gaaaacggta
     6301 atcagttgat gcagcaaaac attaaagtca aaaactggct ggagcgggcg ctgcaatcgg
     6361 aacgcaatat taaagagcag attgccgtcc tgaagggcag cctgctgttg tctcgtatcc
     6421 tttaccaaca acaacaaacg ctgccctcgg cggatgaact ggaaaacatg accaaccgca
     6481 tcgcggattt gcgtctcgaa cagtttgaag ttaaccagca gcgtgatgca ctcttccaga
     6541 gcgatgcgtt cgtcaacaaa ctggaagaag gtcacaccaa cgaagtcaac agcgaagttc
     6601 acgatgcgtt attgcaagtg gttgatatgc gtcgcgaatt gctggatcaa ctcaacaaac
     6661 agttgggtaa ccagctgatg atggccatta acctgcaaat caaccagcag cagttaatga
     6721 gtgtgtcgaa aaacctgaaa tccatcctga ctcagcaaat cttttgggtg aacagtaacc
     6781 gtccaatgga ctgggactgg atcaaagcgt tcccgcaaag cctgaaagat gaatttaagt
     6841 cgatgaaaat tacggtgaac tgggaaaaag cctggcccgc cgtttttatc gctttcctcg
     6901 ctggtttgcc gctgctgttg attgccgggc tgatccactg gcgtctggga tggctgaaag
     6961 cgtatcaaca aaaactggct tccgctgtgg gttccctgcg taacgacagc cagctcaaca
     7021 caccaaaagc gatccttatc gacctgatcc gtgcactgcc ggtgtgcctg attattctcg
     7081 cggttggcct gattctgttg accatgcagc tcaacatcag cgaattgcta tggtcgttca
     7141 gcaaaaaact ggcgattttc tggctggtgt ttggcctgtg ctggaaggta ctggagaaga
     7201 acggcgttgc cgtgcgtcac ttcggcatgc cggagcagca gaccagccac tggcgtcggc
     7261 aaattgtccg catcagtctc gcgttgctgc ctatccattt ctggtctgtg gtggcagaac
     7321 tttctccgct gcatctgatg gatgatgtgc tggggcaggc gatgattttc ttcaacctgc
     7381 tgctgattgc cttcctggta tggccaatgt gccgcgaaag ctggcgtgat aaagagtcgc
     7441 acaccatgcg actggtcacc attaccgtgc tgtcgattat cccgattgca ttgatggtgc
     7501 tgactgccac gggttatttc tacaccacgc tgcgcctctc cgggcgttgg attgaaaccg
     7561 tttatctggt gatcatctgg aacttgctct accagacggt attgcgcggc ttaagcgtag
     7621 cggcgcggcg tatcgcctgg cgtcgtgcgc tggcgcgtcg gcagaatctg gtgaaagagg
     7681 gcgcagaagg tgccgaaccg ccggaagaac ccaccattgc actggaacag gttaaccagc
     7741 agacgctgcg tattaccatg ttgctgatgt ttgcgctgtt cggtgtcatg ttctgggcaa
     7801 tttggtccga tttgatcacc gtgttcagct atctcgacag cattacgctg tggcattaca
     7861 acggtactga agctggtgct gcggtggtga aaaacgtcac catgggcagt ctgttgtttg
     7921 cgattatcgc ctcaatggtg gcctgggcgt tgattcgcaa cctgcctggt ttactggaag
     7981 tgctggtgct ctcgcgactg aatatgcgtc agggcgcgtc gtatgccatt actaccatcc
     8041 ttaactacat cattattgcc gttggtgcga tgacggtgtt cggatcgctg ggcgtctctt
     8101 gggataaact ccagtggctg gccgcagcat tatccgtagg tcttggtttt ggtttacaag
     8161 aaattttcgg taacttcgtc tccggtttga tcattctgtt cgaacgtccg gtgcgtattg
     8221 gcgatacggt aaccattggt agcttctcgg ggacggtaag taagatccgt attcgtgcga
     8281 caacgattac cgatttcgat cgcaaagaag tgatcatccc gaacaaagcg tttgttaccg
     8341 agcgtctgat caactggtcg ttgagtgaca ctactacgcg tctggtgatc cgtctcggcg
     8401 tggcctatgg ctccgatctg gaaaaagtgc gtaaagtgtt actgaaggcg gcgactgagc
     8461 acccaagggt gatgcacgaa ccaatgccgg aagtcttctt tacggcattt ggtgccagca
     8521 cgttggatca tgagctgcgt ctgtatgtgc gtgaactgcg tgaccgtagt cgtactgtcg
     8581 atgagctgaa ccgtactatc gatcagctgt gccgtgaaaa cgacatcaac attgccttta
     8641 accagcttga agtgcatctg cacaacgaga agggcgatga ggtgacggaa gtcaaacgcg
     8701 actacaaagg cgacgatccg acgccagcgg tagggtaata accaaagggg gcgacattta
     8761 gttgccccga gattactgac aaagtgcgcg ttgttcatgc cggatgcggc gtgaccgcct
     8821 tatccggcct acgaaaccgc aagaattcaa tgtattgcag gagcggtgta ggcctgataa
     8881 gcgtagcgca tcaggcagtt ttgcgtttgc ccacaatctt aggggacatt tagcggcccc
     8941 atttatttct cacttttccg cctcatgatc gcgcgttaat ttcttttcat aatcgcgttt
     9001 tacaatatcc agcgcgcgca gcacggtgcg ggctgggatc tgattttctt ccagcagcac
     9061 aatcaaatcg acagccagtt tgacatcgtc aggggcattt tccagtgaca tactctctcc
     9121 attgctagcg ggttaagcgc gctagcctgt tttcgatttt ttccagcgca tggcggcagc
     9181 gtgccaggcg cgcttcatag gcttccactt cacgatgcag cgtttgctgt tccacgagat
     9241 cggtcacccg cgccaggcgg gctctgcgtt cggcaaccat ctcacgtagc cgccgctcaa
     9301 aatcctgatg ctgaatacgt ttacgctgcc agcgggcaat tttcggtggc gcactgtccc
     9361 actcgcgcaa tgaccaggcg gaggcttcac gcgcgatggc ttccagttgt gccgccagat
     9421 gttccgccag ccaggccact tgcggcagct gttgctgctc aactgcatga cgaagcgcag
     9481 ccagattatc gcccgcctcg tcgagacaag cctgtagtgt tgtcgcacga gtcttaaaaa
     9541 gatgcctgtc gaaacgagca cttagcgtgg cgaactgtga caccggggca caacgctgac
     9601 gcagcgtagc gagctgtcct tccagttttt ccagcagcag ggcggttttc acgataagac
     9661 ctcaatgaaa atgataattg ttatgctaaa gtagccactc tgtaagctga caacactcaa
     9721 tatgcaacga atcattttaa tcatcattgg ctggctggcg gtagtgctgg gcacgctggg
     9781 cgtggtatta ccggtattac cgacgacgcc gtttatcctg ctggcggcct ggtgctttgc
     9841 ccgttcttcc ccgcgctttc acgcctggtt gctgtaccgc tcatggtttg gcagctatct
     9901 acgtttctgg cagaaacatc atgcgatgcc gcgcggtgtc aaaccgcggg cgattttgct
     9961 tattttgctc acgtttgcca tttctctgtg gttcgtccag atgccatggg tgcgcatcat
    10021 gttgctggta attctcgcct gtttgctttt ctatatgtgg cgaattccgg tgattgatga
    10081 aaagcaagaa aagcactgaa gcacaacaat cgcagttgca attattgcgt acagccagta
    10141 cattctggcg ttttcgagca caggcgcagg cggtcaaagg ttaaacaact gttacttttg
    10201 atacgtttaa aacgcgccgt gagtaccacc gtaacaagca ggcatacact tatgaccgcg
    10261 actgcacagc agcttgagta tctcaaaaat agcatcaaaa gcattcagga ctacccaaaa
    10321 cccggcattc ttttccgcga tgtcaccagc ttactggaag acccgaaagc ttacgctctc
    10381 agcatcgact tgctggttga gcgttacaaa aatgcgggca ttaccaaagt tgtcggcacc
    10441 gaagcgcgtg gcttcttgtt tggcgctccg gtagctctgg gtctgggcgt tggttttgta
    10501 ccggtccgta aaccgggcaa actgccgcgt gaaactatca gtgaaactta cgatctggaa
    10561 tacggcaccg atcagctgga aatccacgtt gatgccatca aaccgggcga caaagttctg
    10621 gtggtggacg atctgctggc aaccggcggc actatcgaag cgaccgttaa actgatccgt
    10681 cgtctgggtg gtgaagtggc tgacgctgcg ttcattatca acctgttcga tctcggcggc
    10741 gaacagcgtc tcgaaaaaca gggcattacc agctacagcc ttgtcccgtt cccgggccat
    10801 taattatcgc cagtcttgtg ctgcccacgc tacggacagc acaagatgtg cattcagcct
    10861 cgccgttctg acggggctgt gttagcatta ccccttcgtg aatccacctt ccagcgtttc
    10921 agagcctgcc aatgagttat caggtcttag cccgaaaatg gcgcccacaa acctttgctg
    10981 acgtcgtcgg ccaggaacat gtgctgaccg cactggcgaa cggcttgtcg ttagggcgta
    11041 ttcatcatgc ttatcttttt tccggtaccc gtggcgtcgg aaaaacctct atcgcccgac
    11101 tgctggcgaa ggggctaaac tgcgaaaccg gcattaccgc gacgccgtgc ggcgtgtgcg
    11161 ataattgtcg tgaaatcgag caggggcgct ttgtcgatct gattgaaatc gacgccgcct
    11221 cgcgcaccaa agttgaagat acccgcgacc tgctggataa cgtccagtac gctccggcgc
    11281 gtggtcgttt caaagtttat ctcatcgacg aagtgcatat gctgtcgcgt cacagcttta
    11341 acgcactgtt aaaaactctt gaagagccgc cggagcacgt taagtttctg ctggcgacga
    11401 ccgatccaca gaaattgccg gtgacgattt tgtcgcgctg tttgcaattt catctcaagg
    11461 cgctggatgt cgagcaaatt cgtcatcaac ttgagcacat cctcaatgaa gaacatatcg
    11521 ctcacgagcc gcgggcgctg caattgctgg cgcgcgccgc tgaaggcagc ctgcgagatg
    11581 ccttaagtct gaccgaccag gcgattgcca gcggtgacgg ccaggtttca acccaggcgg
    11641 tcagtgcgat gctgggtacg cttgacgacg atcaggcgct gtcgctggtt gaagcgatgg
    11701 tcgaggccaa cggcgagcgc gtaatggcgc tgattaatga agccgctgcc cgtggtatcg
    11761 agtgggaagc gttgctggtg gaaatgctcg gcctgttgca tcgtattgcg atggtacaac
    11821 tttcgcctgc tgcacttggc aacgacatgg ccgccatcga gctgcggatg cgtgaactgg
    11881 cgcgcaccat accgccgacg gatattcagc tttactatca gacgctgttg attggtcgca
    11941 aagaattacc gtatgcgccg gaccgccgca tgggcgttga gatgacgctg ctgcgcgcgc
    12001 tggcgttcca tccgcgtatg ccgctgcctg agccagaagt gccacgccag tcctttgcac
    12061 ccgtcgcgcc aacggcagta atgacgccaa cccaggtgcc gccgcaaccg caatcagcgg
    12121 cgcagcaggc accgactgta ccgctctcag aaaccaccag ccaggtgctg gcggcgcgcc
    12181 agcagttgca gcgcgtgcag ggagcaacca aagcaaaaaa gagtgaaccg gcagccgcta
    12241 cccgcgcgcg gccggtgaat aacgctgcgc tggaaagact ggcttcggtc acggatcgcg
    12301 ttcaggcgcg tccggtgcca tcggcgctgg aaaaagcgcc agctaaaaaa gaagcgtatc
    12361 gctggaaggc gaccactcca gtgatgcagc aaaaagaagt ggtcgccaca ccgaaggcgc
    12421 tgaaaaaagc gctggaacat gaaaaaacgc cggaactggc ggcgaagctg gcggcagaag
    12481 ccattgagcg cgacccgtgg gcggctcagg tgagtcaact ttcgctacca aaactggtcg
    12541 aacaggtggc cttaaatgcc tggaaagagg agagtgataa cgcagtatgt ctgcatttgc
    12601 gctcctctca gcggcatttg aacaaccgcg gtgcacagca aaaactggct gaagcgttga
    12661 gcatgttaaa aggttcaacg gttgaactga ctatcgttga agatgataat cccgcggtgc
    12721 gtacgccgct ggagtggcgt caggcgatat acgaagaaaa acttgcgcag gcgcgcgagt
    12781 ccattattgc ggataataat attcagaccc tgcgtcggtt tttcggtgcg gagctggatg
    12841 aagaaagtat ccgccccatt tgatcgtaag cacagcttac gttcgtcatc cttaacgtga
    12901 ttgagagaga aacctatgtt tggtaaaggc ggtctgggta acctgatgaa gcaagcccag
    12961 cagatgcaag aaaaaatgca gaaaatgcag gaagagatcg cgcagctgga agtcaccggc
    13021 gaatctggcg caggtctggt aaaagtgacc atcaacggtg cacacaactg ccgtcgcgta
    13081 gagatcgacc cgagcctgct ggaagacgac aaagagatgc tagaagacct ggtggctgca
    13141 gcattcaacg acgcagcacg tcgtattgaa gaaacgcaga aagaaaaaat ggcctctgta
    13201 tcctccggaa tgcagctgcc gcctggcttt aagatgccgt tctgatgcaa accagcccgc
    13261 tgttaacaca gcttatggaa gcactgcgct gtctgccggg cgttggcccg aagtcggcgc
    13321 agcgtatggc gttcacgctg cttcagcgcg atcgtagcgg cgggatgcgt ctggcgcagg
    13381 cgctcacccg ggcgatgtcg gaaatcggcc actgcgccga ttgccgcacc ttcaccgaac
    13441 aggaagtctg taacatctgt tcgaatccgc gtcgtcagga aaacggtcaa atctgcgtgg
    13501 tggagagtcc ggcggacatt tacgccattg agcagacggg gcagttttca ggtcgttatt
    13561 ttgtgttgat gggacatctg tcaccgctgg acggcatcgg tccggatgat atcggccttg
    13621 atcgtctgga acagcgtctg gcagaggaaa aaatcactga agtgatcctc gccaccaacc
    13681 ccacggttga aggtgaagct accgctaact acattgccga gctttgcgcg caatatgacg
    13741 tggaagccag ccgaatcgct catggcgtac cggttggcgg cgaactggaa atggtcgatg
    13801 gcaccacgct gtcacactcc cttgccgggc gtcataagat tcgtttttaa gcaaacgaga
    13861 gcaggatcac ctgctctcgc ttgaaattat tctcccttgt ccccatcttt ccctcatcct
    13921 gtttttaacc ttaaaatggc attattgagg tagacctaca tgaaaggaca agaaactcgt
    13981 ggttttcagt cagaagtgaa acagcttctg cacctgatga tccattctct ctattccaat
    14041 aaagaaatct tcctgcgtga gcttatctct aacgcctccg atgcggcgga caagctgcgt
    14101 ttccgcgcgc tctctaaccc ggacctgtac gaaggtgatg gcgaactgcg cgttcgtgtc
    14161 tctttcgata aagacaagcg cacgctgacc atctccgata acggcgtggg gatgacccgc
    14221 gacgaagtga ttgaccatct ggggactatc gctaaatccg gtaccaaatc attcctcgaa
    14281 tccctgggtt ctgaccaggc gaaagacagc cagctgatcg gtcagtttgg tgttggtttc
    14341 tactctgcgt ttatcgtggc cgacaaagtg accgtgcgta ctcgcgcggc aggcgaaaaa
    14401 ccagaaaatg gcgtcttctg ggaatcggct ggcgaaggtg aatacaccgt tgccgacatc
    14461 accaaagaag atcgtggtac tgaaatcacc ctgcatctgc gtgaaggcga agacgagttc
    14521 ctcgatgact ggcgcgtgcg ttccatcatc agcaaatact ccgaccatat cgcgctgccg
    14581 gtagagatcg aaaaacgcga agagaaagac ggcgaaaccg ttatctcctg ggagaaaatc
    14641 aacaaagcgc aggcgctgtg gactcgtaac aagtcggaaa tcaccgatga agagtataaa
    14701 gagttctaca aacacatcgc ccacgacttt aacgatccgc tgacctggag ccacaaccgc
    14761 gttgaaggta agcaggagta caccagcctg ctgtacatcc cgtcccaggc tccgtgggat
    14821 atgtggaacc gtgatcataa acacggcctg aaactgtatg ttcagcgtgt gttcatcatg
    14881 gacgacgcag aacagttcat gccgaactat ctgcgcttcg tgcgtggtct gattgactcc
    14941 agcgatctgc cgctgaacgt ttcccgtgaa atcctccagg acagcacggt aacgcgtaac
    15001 ctgcgcaatg cgctgaccaa gcgtgtgctg caaatgctgg aaaaactggc gaaagacgac
    15061 gcggaaaaat accagacctt ctggcaacag tttggcctgg tactgaaaga aggtccggcg
    15121 gaagatttcg ctaaccagga agcgatcgcc aaactgctgc gttttgcttc tactcatacc
    15181 gattcttctg cgcagaccgt gtctctggaa gactacgttt cccgcatgaa agaagggcag
    15241 gagaaaatct actacatcac tgcagacagc tatgcggcag cgaagagcag cccgcacctg
    15301 gaactgctgc gtaagaaagg catcgaagtt ctgctgcttt ccgaccgcat cgatgagtgg
    15361 atgatgaact atctgactga gttcgacggt aaaccgttcc agtcggtgtc taaagttgac
    15421 gagtcgctgg aaaaactggc tgacgaagtt gatgagagcg cgaaagaagc ggagaaagca
    15481 ctgactccgt tcatcgaccg cgtgaaagct ctgctcggcg agcgcgtgaa agatgtccgt
    15541 ctgactcacc gtctgaccga tacgccagcg atcgtttcga ccgacgcgga cgaaatgagc
    15601 actcagatgg cgaaactgtt cgctgcggcg ggccagaaag tgccagaagt gaaatacatc
    15661 ttcgaactga acccggatca cgtactggtg aaacgtgcgg cagatactga agatgaagcc
    15721 aagttcagcg agtgggtaga actgctgctg gatcaggcgc tgctggcaga acgcggcacg
    15781 ctggaagatc cgaacctgtt tattcgtcgt atgaaccagc tgctggtttc ctgatgtaat
    15841 gccggatgac cttcgtgtca tccggcattt ttcttttcat tatctgcact ttccgcaaat
    15901 tatctcgcca ttaaccgttt cagcccctgg tgcctttctt gaggcaatcg cctgatggtg
    15961 gtatcgttta tcgctttttc aaaaaattcg acacatttta aggggatttt cgcaatgcgt
    16021 atcattctgc ttggcgctcc gggcgcgggg aaagggactc aggctcagtt catcatggag
    16081 aaatatggta ttccgcaaat ctccactggc gatatgctgc gtgctgcggt caaatctggc
    16141 tccgagctgg gtaaacaagc aaaagacatt atggatgctg gcaaactggt caccgacgaa
    16201 ctggtgatcg cgctggttaa agagcgcatt gctcaggaag actgccgtaa tggtttcctg
    16261 ttggacggct tcccgcgtac cattccgcag gcagacgcga tgaaagaagc gggcatcaat
    16321 gttgattacg ttctggaatt cgacgtaccg gacgaactga tcgttgaccg tatcgtcggt
    16381 cgccgcgttc acgcgccgtc tggtcgtgtt tatcacgtta aattcaatcc gccgaaagta
    16441 gaaggtaaag acgacgttac cggtgaagaa ctgactaccc gtaaagacga tcaggaagaa
    16501 accgtacgta aacgtctggt tgaataccat cagatgacag caccgctgat cggctactac
    16561 tccaaagaag cggaagcggg taataccaaa tacgcgaaag ttgacggcac caagccggtt
    16621 gctgaagttc gcgctgatct ggaaaaaatc ctcggctaat caagctgcag cagatctgct
    16681 ttcgggcagg tctgctcatc cacctcaggc aatacctctc acagcaatta gttcttcttc
    16741 ctcacttttc cgctacaatt atcaacaagt tgaatcgata agaggcggta atgcgtcaga
    16801 ctaaaaccgg tatcctgctg gcaaacctgg gtacgcccga tgcccccaca cctgaagcgg
    16861 taaaacgcta tctgaaacaa tttttaagcg acagacgcgt ggttgatacc tcacggttgt
    16921 tatggtggcc gttgctgcgc ggcgtgattt tgccgctgcg ctcgccgcgt gtggcgaagc
    16981 tgtatgcctc tgtctggatg gaaggtggct cgccgctgat ggtttacagc cgccagcaac
    17041 agcaggcgct ggcacaacgt ttaccggaga cgcccgtagc gctgggaatg agctacggct
    17101 cgccatcact ggaaagcgcc gtagatgaac tcctggcaga gcatgtagat catattgtgg
    17161 tgctgccgct ttatccgcaa ttctcctgtt ctacggtcgg tgcggtatgg gatgaactgg
    17221 cacgcattct ggcgcgcaaa cgtagcattc cggggatatc gtttatacgt gattacgccg
    17281 ataaccacga ttacattaat gcactggcga acagcgtacg cgcttctttt gccaaacatg
    17341 gcgaaccgga tctgctgctg ctctcttatc atggcattcc ccagcgttat gcagatgaag
    17401 gtgatgatta cccgcaacgt tgccgcacaa cgactcgcga actggcttcc gcactgggga
    17461 tggcaccgga aaaagtgatg atgacctttc agtcgcgctt tggccgggaa ccctggctga
    17521 tgccttatac cgacgaaacg ctgaaaatgc tcggagaaaa aggcgtaggt catatacagg
    17581 tgatgtgccc gggctttgct gcggattgtc tggagacgct ggaagagatt gccgagcaaa
    17641 accgtgaggt cttcctcggt gcgggcggga aaaaatatga atatattccg gcgcttaatg
    17701 ccacgccgga acatatcgaa atgatggcta atcttgttgc cgcgtatcgc taaagctgag
    17761 cggtaaagaa ctgagcgccg tcgcgaagag tctcgtcggc ggttttcatc atccgtgaat
    17821 aatgcaaaaa ggcgtgcagc gtgcctgggt agagtttgaa ctcacagggc tgctgatgcg
    17881 ccgctaacgt ctggtaaagc agacggctgt catccagcag cggatcgaac tccgccccgg
    17941 caataaaaca gggcggcact tcgcgagtga gatcattatt aaacagacag taatacggcg
    18001 actcgcggtc cgcgtcgttg cttaaatacg cctcttcata catctgcaaa tcctgttgcg
    18061 ttaagccatc ccagacaccg cccaacagac gacgagtcac ggaatcccgt aatccgtaaa
    18121 gcccatacca cagcaaaaca cccgcaattt taccgcaatc gatctgttta tcacgcaacc
    18181 acaacgcact ggcgagcgcc agcatggcac ctgcggaatc accggcaaag ccaatgcggg
    18241 acatattgat ttgataatcc tccgcctgct ggtggaaata acaacaggca gccacaattt
    18301 cctctatcgc ttgcggaaaa cgcgcttcag gtgaaagggt gtaatcaatg ccaatcaccg
    18361 tacattggct gtagcttgcc agcaggcgca tgatgcgatc atgggtatcg agattgccga
    18421 gaataaaacc gcctccatgc aaataaaata gcgttgccgg gctatctggc tgcggacaaa
    18481 agagacgtgt ttccacctgc ccatattttg ttggaaccat gtaagctctg gttgccattt
    18541 ctggagcgcc cgcattccag aatcggcgct caagcgtgta atactgtcgt tgctcagcaa
    18601 tcgttcccgt tgcgggccag ggcggtaaat ccggctgaag agtattcaca acggtcttca
    18661 tttcagcaga aataaggtcc agaacaggta atttgttttc cggcttcata caaaaactcc
    18721 tttcaaatta cgtcattgta aggaaccact gccatgaaaa tgcgatcccg cctgctgata
    18781 ttgaaactgg ctgcgtctcg cgcgctcccg tcagattgtg ttaacattcg ccgctcagtt
    18841 aaccacccgt aaaaacaacc atgaaatttc ccggtaaacg taaatccaaa cattacttcc
    18901 ctgtaaatgc acgcgatccg ctgcttcagc aattccagcc agaaaacgaa accagcgccg
    18961 cctgggtcgt aggtattgac cagacgctgg tcgatattga agcgaaagtg gatgatgaat
    19021 tcattgagcg ttatggatta agcgccgggc attcactggt gattgaggat gatgtagccg
    19081 aagcgcttta tcaggaacta aaacagaaaa acctgattac ccatcagttt gcgggtggca
    19141 ccattggtaa caccatgcat aactactcgg tgctcgcgga cgaccgttcg gtgctgctgg
    19201 gcgtcatgtg cagcaatatt gaaattggca gctatgccta tcgttacctg tgtaacacct
    19261 ccagccgtac cgatcttaac tatctacaag gcgtggatgg cccgattggt cgttgcttta
    19321 cgctgattgg cgagtccggg gaacgtacct ttgctatcag ccctggccac atgaaccagc
    19381 tgcgggctga aagtattccg gaagatgtga ttgccggagc ctcggctctg gttctcacct
    19441 cttatctggt gcgttgcaag ccgggtgaac ccatgccaga agcaaccatg aaagccattg
    19501 agtacgcgaa gaaatataac gtaccggtgg tgctgacgtt gggcaccaag tttgtcattg
    19561 cagagaatcc gcagtggtgg cagcaattcc tcaaagacca cgtttctatc cttgcgatga
    19621 acgaagatga agccgaagcg ttgaccggag agagcgatcc gttgttggca tctgacaagg
    19681 cgctggactg ggtagatctg gtgctgtgca ccgccgggcc aatcggcttg tatatggcgg
    19741 gctttaccga agacgaagcg aaacgtaaaa cccagcatcc gctgctgccg ggcgctatag
    19801 ccgaattcaa ccagtatgag tttagccgcg ccatgcgcca caaggattgt cagaatccgc
    19861 tgcgtgtcta ttcgcacatt gcgccgtaca tgggcgggcc ggaaaaaatc atgaacacca
    19921 acggagcagg ggatggcgca ctggcagcgt tgctgcatga cattaccgcc aacagctacc
    19981 atcgtagcaa cgtaccaaac tccagcaaac ataaattcac ctggttaact tattcatcgt
    20041 tagcgcaggt gtgtaaatat gctaaccgtg tgagctatca ggtactgaac cagcattcac
    20101 ctcgtttaac gcgcggcttg ccggagcgtg aagacagcct ggaagagtct tactgggatc
    20161 gttaagttat cgtcggttcg taggccagat aaggcgttca cgccgcatct ggcatttggc
    20221 tctcgatgcc tgatgcgacg ctggcgcgtc ttatcatgcc tacatatttt tcatatttta
    20281 catccggcaa ccaccgttta ccccgtcacc acttcacccg ccggtggcgt ttccagcagt
    20341 tccagcatag tacgggcaat ttcacgctcg cccatcacta cctgattcgc accacgttcg
    20401 gtgatatacg ccacttcatc gtcataatgg gcgcgggcaa taatctcaat atccggattt
    20461 ttcgcgcggg cagatgccac aatctcaccc gcttcataac cgttggggat cgtcaggatc
    20521 agccattttg cacattccag atgcgccagt tgcataattt cttcgttcgc tgcattgccc
    20581 aatactgcgc ggaccccgcg ctctcgcagc tcatcaacac gggttcgtga cgtctcaatc
    20641 accaccagcg gaatatcaga ggcgagcaat ttctccccca gcaggctgcc tacacgaccg
    20701 taacccacca gtagcgcatg gttgcaaata tccactggga tctgtttctc ttcttcgatt
    20761 gcctcttcca gcgtctgctc ttccagcgtt tcggtcttcg ccagatattt ctccagtaat
    20821 gcgaacagga ccgggttgag cataatcgac aaaatcgcac ctgccagtac caggttctgc
    20881 ccggcttgcg gcaacagatc cagcgccatg cccagtcccg ccaggataaa cgcgaactca
    20941 ccaatttgcg ccaggctggc ggcgatagtt aatgccgtac gttgggagtg accaaacagt
    21001 cgcaccagga aaaacgcggc taacgactta ccaaacagaa taattgccag cgtcgccagt
    21061 actgccagcg gttgctgaat cagaattaac ggatcaaaca acatcccgac ggagacaaaa
    21121 aacagcaccg caaacgcgtc gcgcaatggc agcgtatcgt gggcggcacg gtgactcagt
    21181 tcagactcgt tcagtaccat cccggcaaag aacgcaccga gtgcaaagga gacatcaaac
    21241 agctccaccg caccaaaggc aatccctaac gccagcgcca gcaccgacag ggtaaacagc
    21301 tcgcgagaac cggttgccgc gctgcgcgcc ataatccacg gcaccagacg gcgacctacc
    21361 agcatcataa tggcgataaa tgcgatcacc ttgccgatgg tgatccccat atcgactgca
    21421 agagtggcaa agcccacatc gccctgttcc atcattcctg ccactgcggg cagcaacacc
    21481 agcgtcagaa ccattaccag gtcttccaca atcaaccaac cgatggcgat ttgcccacgc
    21541 tgactgtcaa ttaattgccg ttcttcaagt gcgcgcagta acaccacggt actggcggtg
    21601 gaaagacata aaccgaacac gataccggtc attaacgacc agcccagcac ggcagagagc
    21661 gccataccca gcagcgtcgc cacggctatc tgggcgatcg caccgggaat ggcgatggcc
    21721 tttaccgcca tcaaatcctt cagcgaaaag tgcagaccca cgccaaacat caacagaatg
    21781 acgcccagtt cagccagttc cggcgcaagc ttggtatcgg caacaaagcc cggagtgaat
    21841 ggtcctgcca gcacacccgc taacagatat cccaccagag gagaaatacg tagtttattg
    21901 gccagcatgc cgaggataaa ggcgagcaca aggccgccaa caatggtggt gataagcggg
    21961 gtggcgtgat gcattccgtc tccttttcct ggtggttatt gtccattttt ggccgggaaa
    22021 accaaaatta caggtaatag tttatgacaa tttcattgat gatgttcatg aataattatt
    22081 gaattttgca gaaaaatgga attagctgca aaaaaaaaag tacggatcgg aaaacagaag
    22141 gcggtttaac agaggaaagg cttatggcga ctgtaaaacg atgcagccaa agtttgcctt
    22201 tggctgcaat gaaatcagtc tttatgccgg ctatcaggca ggaatatggt caacatcccc
    22261 aatagtggca ggaaagcaca gattttatag actaactcga tgctggtgtg atcggcgata
    22321 agccccagaa ctgccgctcc cagacctccc atgccaaaag caaaaccgaa aaagagtcca
    22381 gaaaccatac cgatacgtcc tggaagcagc tcctgagcgt agaccagaat ggcagagaat
    22441 gccgaagcga ggataaatcc aataatcacc gttaaaaccc ccgtccagtg caggctggcg
    22501 tagggtaaaa tcagcgtaaa cggcgcaacg ccgaggatag agccccaaat cacatatttc
    22561 ctcccaattt tatcccctac aggcccgccg atcaccgtac ctgccgcaac ggcaaacagg
    22621 aaggcaaaca gatgaagctg agcattctgg atagataatc cgaatttttg catcagataa
    22681 aaggtgtaat agctgctgat gctcgccata tagaaatatt tcgagaaaat gaggattaac
    22741 agaatactga ccgccagtac cactttgttg cgcggcagtg gattgataat cgtcgctttg
    22801 ggttttcctt tattcattcg gtgctgtgcc gagtaccaac ggctgatttg cgccaacacc
    22861 acgatcgcca gcagtgccgc aagcacaaac caggcaacgt tgcctttgcc ataaggcgcg
    22921 ataatcaccg ccgccagcaa ggggcccagg gaactgccaa agttgccgcc gacctgaaag
    22981 atagattgtg ccaggccatg ccgcccgccg gaagccatac gggccacgcg agaagattcc
    23041 ggatgaaaga ccgatgaacc ggtaccgacc agcgccgccg ccagcagaac tgcgccaaaa
    23101 ctgcccgcca gcgcaagcag caccagacca cttaaggtaa agcacatgcc aattggcaac
    23161 gaccacggca tcggatattt atcggtccag tagccgacca ctggttgcag tagcgaagag
    23221 gcgagctgga aggtgagggt tatcatgcca atctgcataa atgtcagaga aaattctgac
    23281 tgaagcagag gataaatcgc cagaatcagc gattggatca tgtcgttcag cagatgtgag
    23341 aggctgatag cacctaaaat accaaacgat gttcgggcct tggtcgttga cgcagccgcg
    23401 cccgccacag gctgggtttg ttcactcatt gccataggaa agtcactttt tcagggttgc
    23461 gatgtaaaga atgatcttat ttgtgattat tacccgacta acatacctgt atgcgccgcc
    23521 tgaaggaagt ctcaacgctg aatacagaat ttctaatctg gatgcagatt tatcttcacc
    23581 ggacgcagac ttgtctatga tgtcgcgtca tactattttt caacacgttg aaatcaggtc
    23641 agggagagaa gtatgaaatt attgcagcgg ggcgtggcgt tagcgctgtt aaccacattt
    23701 acactggcga gtgaaactgc tctggcgtat gagcaggata aaacctacaa aattacagtt
    23761 ctgcatacca atgatcatca tgggcatttt tggcgcaatg aatatggcga atatggtctg
    23821 gcggcgcaaa aaacgctggt ggatggtatc cgcaaagagg ttgcggctga aggcggtagc
    23881 gtgctgctac tttccggtgg cgacattaac actggcgtgc ccgagtctga cttacaggat
    23941 gccgaacctg attttcgcgg tatgaatctg gtgggctatg acgcgatggc gatcggtaat
    24001 catgaatttg ataatccgct caccgtatta cgccagcagg aaaagtgggc caagttcccg
    24061 ttgctttccg cgaatatcta ccagaaaagt actggcgagc gcctgtttaa accgtgggcg
    24121 ctgtttaagc gtcaggatct gaaaattgcc gttattgggc tgacaaccga tgacacagca
    24181 aaaattggta acccggaata cttcactgat atcgaatttc gtaagcccgc cgatgaagcg
    24241 aagctggtga ttcaggagct gcaacagaca gaaaagccag acattattat cgcggcgacc
    24301 catatggggc attacaataa tggtgagcac ggctctaacg caccgggcga tgtggagatg
    24361 gcacgcgcgc tgcctgccgg atcgctggcg atgatcgtcg gtggtcactc gcaagatccg
    24421 gtctgcatgg cggcagaaaa caaaaaacag gtcgattacg tgccgggtac gccatgcaaa
    24481 ccagatcaac aaaacggcat ctggattgtg caggcgcatg agtggggcaa atacgtggga
    24541 cgggctgatt ttgagtttcg taatggcgaa atgaaaatgg ttaactacca gctgattccg
    24601 gtgaacctga agaagaaagt gacctgggaa gacgggaaaa gcgagcgcgt gctttacact
    24661 cctgaaatcg ctgaaaacca gcaaatgatc tcgctgttat caccgttcca gaacaaaggc
    24721 aaagcgcagc tggaagtgaa aataggcgaa accaatggtc gtctggaagg cgatcgtgac
    24781 aaagtgcgtt ttgtacagac caatatgggg cggttgattc tggcagccca aatggatcgc
    24841 actggtgccg actttgcggt gatgagcgga ggcggaattc gtgattctat cgaagcaggc
    24901 gatatcagct ataaaaacgt gctgaaagtg cagccattcg gcaatgtggt ggtgtatgcc
    24961 gacatgaccg gtaaagaggt gattgattac ctgaccgccg tcgcgcagat gaagccagat
    25021 tcaggtgcct acccgcaatt tgccaacgtt agctttgtgg cgaaagacgg caaactgaac
    25081 gaccttaaaa tcaaaggcga accggtcgat ccggcgaaaa cttaccgtat ggcgacatta
    25141 aacttcaatg ccaccggcgg tgatggatat ccgcgccttg ataacaaacc gggctatgtg
    25201 aataccggct ttattgatgc cgaagtgctg aaagcgtata tccagaaaag ctcgccgctg
    25261 gatgtgagtg tttatgaacc gaaaggtgag gtgagctggc agtaatccga aagtgccgga
    25321 tgtttgcatc cggcacaatg cttaatcgcg gcgggcgata tcagcaaatt tggcatcgag
    25381 gatctttgcc agatcgcctg ccgccagttc gatatccagt ccgcgcttgc cgccggaaac
    25441 ataaatagtg gcaaattctt gtgcgggggc gtcgataatc gttggcagac gttttttctg
    25501 ccccagtggg ctaatccccc caaccaggta tcccgtcgaa cgctgcgcga ccatcggatc
    25561 ggccatctca actttcttgg cacccagcgc ttttgctact tttttaagat ccagttgacc
    25621 tgcgaccggc gtaacggcca cggcaaggtg tttcatatca ccgttcactg ccaccagcag
    25681 cgttttgtag acctgatccg gattcaaacc taattttttg acgacttcat cgccaaaatt
    25741 ggtttcagcc ggatcgtgct cgtaggtatg gatttgaaac gaaatcttgt ttttttcgag
    25801 taatttaact gcgggtgtca tggtaatttt cctgccttaa acaaaaaaac gaagcaagca
    25861 tacgcctgat ggcggtccaa aaaaaagagt catcttgcct aagagtattg gcaggatggt
    25921 gagattgagc gacaatcgag ttacaccgtc gcttaaagtg acggcataat aataaaaaaa
    25981 tgaaattcct ctttgacggg ccaatagcga tattggccat ttttttagcg caacatttgc
    26041 ggtaaattcc cttctccata caggtgtagt gcaccgaccg cgaccacata tcgccccggc
    26101 ggcatggcgc gtaatttatc ccgccaggcg agatttcgct gatgcatcag cacatcgtac
    26161 agcgactgac tgaacgtatt gggcagcgtt atttcattat tttgcggcgg cgcattcagc
    26221 caccagctca tcatttgttg cagcaaccgt gcgttggtat gccagtgggt cagcgtatcg
    26281 tccagcagcg ccagtccttt gtcagggagc tggagcaaca tggcaatctg gttttcagcc
    26341 ccttccagtt caatcacggg tttatgttgt tgcttcgccg cctgcaatag ctggtaatcg
    26401 ataccgtatt ctgcccgcag ccccagtttt tgtgcctgcg tcgcctgaag aaccatcgcg
    26461 atttgccaca gcggttgagt agaaaagagc gacggagaaa tgcccatctc ctggctaatg
    26521 tgctgcagat tttggagttg ctcctcgcta atgcgctctt ccagcgcctc gcaagtaggc
    26581 aaattagcaa aaggcgtatc gctggtggaa acatccgcct cgacgatcag cgcatcggcg
    26641 tttttgagct ttttgagcaa acgggtgggc aggggagcca tatcgtggct acccatatga
    26701 atactgccaa tcagatgaaa atggcgattg ccggggaggg tgatatcgat ggcaggccag
    26761 gtgtaatgat taccgcgcag cgcggcccaa agcgttttta cccggtacaa cagatccata
    26821 cgacctccct ttgtgaaata tcatgctagc gcgcggggag ggatggcgca aatggagttg
    26881 gggacttgtt taatgtgttt gtatgattca gtatgttctt gtatcgctat tcacagggaa
    26941 gcaacagtta aaaactatga aacaggcaac aagaaaaccg acgacacccg gagatattct
    27001 tctctatgaa tatccggaac agatcgcttt gaaaatcaat gagttagtag agttgctgca
    27061 tgttcatcgt aatagcgtca gtacactgat caataataat cgtaaactca ccactgaaat
    27121 ggcattccgt ctggcgaaag tttttgatac cacagtcgat ttttggctaa acctccaggc
    27181 ggcagttgat ctttgggaag ttgaaaacaa catgcgcacc caggaagaat tgggacggat
    27241 tgaaacagtg gctgaatatt tggcacgccg tgaagagcgt gcaaaaaagg tcgcgtaagg
    27301 ccaaaacatc gcttcatctc gtaggccgga taaggcgttc acgccgcatc cggcatccga
    27361 gcaccattgc ctgatgcgac gcttacgcgt cttatcaggc ctacaaacct gtgcctgaac
    27421 cgtaggccgg ataaggcgtt tacgccgcat ccggcatccg agcaccattg cctgatgcga
    27481 cgcttacgcg tcttatcagg cctacaaacc tgtgcctgaa ccgtaggccg gagaaggcgt
    27541 tcacgccgca tccggcatcc gagcaccatt gcctgatgcg acgcttacgc gtcttatcag
    27601 gcctacaaac ctgtgcctga accgtaggcc ggataaggcg ttcacgccgc atccggcagt
    27661 catgcgtcaa tgccaaatgc gccgccctaa agcagcgcat ccgcaatgac gtacttattc
    27721 cttcggttta aaccgcagca accggttggc gttactcact acggtaatcg acgagagcgc
    27781 cattgccgct ccggcaacta ccgggttaag cagtgttcca gtaaacggcc acaaaatacc
    27841 ggcggcgacc ggaataccga tactgttgta gataaacgcg ccgagcaggt tctgcttcat
    27901 gttgcgcagc gttgcgcggg aaatagcgag cgcatccgca acgcccatca ggctatggcg
    27961 catcagggta atcgccgcgg tttcaatggc aacatcacta ccgcccccca tcgcaatgcc
    28021 gacatccgcc tgggccagcg ctggcgcatc gttaatgccg tcgcccacca ttgccacctg
    28081 acgtccttca ctttgcagac gtttgatcgc ttcggcttta ccgtccggca gcaccccggc
    28141 tatcacctca tcaatcccgg cttctttggc gatcgcattg gcggtggttg ggttatcccc
    28201 ggtcaacatc accagacgat atcccgcttt gtgcaggcgt tgcagcgccg ccacgctatc
    28261 actacgcaat ggatcgcgta cagccagcag ggctaccgct ttcccgtcaa ccgccagcag
    28321 cacaggcgtt gccccttgcg atgcctgagc tgaaatatct gcttcaatcg ctttagtatc
    28381 aacttgctgg tcatttaaca gcgcctgatt gcccagcaat aacgcatgac cttcagcttc
    28441 accgctcacg cccagcccgc gcaatgtgcg gaaaccgttg acctgcggca gctgcatatc
    28501 gcttgcttta tcgaggatcg cccgtgccag cggatggctg gaaccttgtt ccagtgccgc
    28561 cgccagacgc agcgcctgcg cttcgtcaaa atcagcaaat gttttcactg cgacaacctg
    28621 cggcttccct tcggtcagcg tcccggtttt atcgaacacg acggtatcga gtgtactggc
    28681 gcgttgcagc gcgtcggcgt cccgcaccag cacgccaaac tcagccgccc gcccgacgcc
    28741 ggaaataatc gacatcggcg tcgccagccc cagcgcacac ggacaggcaa taatcagcac
    28801 cgtggtggca atcaccaggg tatagacaat ctgcggtgcc ggaccaaaga aataccagat
    28861 tgccgcactg atcagcgcaa tcaccactac taccggcaca aatacggctg agattttatc
    28921 cgccagctga ccgatttctg gcttgctgct ctgggcctgg cgcaccatac gaataatcct
    28981 tgataacgtg gtatggctgc caaccgcact ggcgcgaaac agaacgctac cgtcctgtac
    29041 cactgtcccg gcatggacgc tatcgccttc gcctttttgc tgcgggatag gttcgcccgt
    29101 cagcatcgct tcatccagcc atgcttcgcc ctgggtaatc tcgccatcta ctggcacgcg
    29161 gtcgcccgtc gtcaggcgca gtagcatgcc tggctgaact tccgccagcg gcacgctttt
    29221 ttcaccttcg tcagtaacca ggcgcgccgt cggcggagtt aaatcgagta atttctccag
    29281 cgcctttgaa gaacgctgac gtgcgcgcgc ttccagcata tgaccgagat taatcagacc
    29341 gataatcatc gcactggctt catagtaaag atgccgcgct tccatcggga accactgcgg
    29401 ccacaggttg acgctcatcg aatagagcca cgccacaccc gtacccagcg ccaccagcgt
    29461 atccatcgtc gccgcaccgt tcagcaggct tttccatgca ctgcggtaaa aatggccgcc
    29521 ggcgaaaacc atcactgcca gggttatcag gccgataacc aaccacaagc tgcggttgtc
    29581 agcggtgacc atcatgttat cgccgatcat cccccagacc attaccggga tacccaccgc
    29641 cagtgcgaca atcgcctgcc agcggaagcg cttcatcgta gcgacggcgg tttcctgctg
    29701 gcgctcgcgg cgtttagcgt catcttcaat cgcttccgcg ccgtagcccg ctttttccac
    29761 cgcctgcact aaatcttgtg gggaggcact gcccatcacc agcgcagtac gctccgccag
    29821 gtttacccgt gcctgagtga cgcccggtac gctttgcagc gcattttgta cgcgggtgac
    29881 acagctggcg cagctcatgc cgctcagcag caactgctgg ctgtcatcgt catcggcggt
    29941 cgctgccgga agcgcctcag aaaccgctgt cagtgcttcc gacgggattg atgactccgc
    30001 cagcggttta gcctttgggt ggcttacaga tgcgtcataa cccgcttgtt tgatggtttc
    30061 gatcagctgt tctgcactgg cagtcccggt aacgtgcgct tcagtgatag acacatccgc
    30121 ttgttccaca tccgggcgct gttcgagact ttctttcacg cgtttaacgc agtgaccgca
    30181 ggacaggccg tccagggtca ggtcgatagt ttgtgacata aaacactcct ttaagacagt
    30241 tttgactggc tgtgataaag gttaaacctt ccagcaaggg gaaggtcaag aaattaataa
    30301 accaggcgag taaaagtccg taaagattaa aaaattggct caatttgcat caggattaga
    30361 catttatctc tttgttttcc tgtagttaag ttgcgggtgc taagttaaat caggatgcct
    30421 caaaatcggc accggggtga ggaattacct cccgcatcta taaaaaggag ttaacaaaag
    30481 atgttagatg caaacaaatt acagcaggca gtggatcagg cttacaccca atttcactca
    30541 cttaacggcg gtcaaaatgc cgactacatt ccctttctgg cgaatgtgcc tggtcaactg
    30601 gcggcagtgg ctatcgtgac ctgcgatggc aaggtctata gtgcgggtga cagtgattac
    30661 cgctttgcgc tggaatccat ctcgaaagtc tgtacgttag cccttgcgtt agaggatgtc
    30721 ggcccgcagg cggtacagga caaaattggc gctgacccga ccggattgcc ctttaactca
    30781 gttatcgcct tagagttgca tggcggcaaa ccgctttcgc cactggtaaa tgctggcgcc
    30841 attgccacca ccagcctgat taacgctgaa aatgttgaac aacgctggca gcgaatttta
    30901 catatccaac agcaactggc tggtgagcag gtagcgctct ctgacgaagt caaccagtcg
    30961 gaacaaacaa ccaacttcca taaccgggcc attgcctggc tgctgtactc cgccggatat
    31021 ctctattgtg atgcaatgga agcctgtgac gtgtacaccc gtcagtgctc cacgctcatc
    31081 aatactgttg aactggcaac gcttggcgcg acgctggcgg cgggtggtgt gaatccgttg
    31141 acgcataaac gcgttcttca ggccgacaac gtgccgtaca ttctggccga aatgatgatg
    31201 gaagggctgt atggtcgctc cggtgactgg gcgtatcgtg ttggtttacc gggcaaaagc
    31261 ggtgtaggtg gcggtattct ggcggtcgtc cctggcgtga tgggaattgc cgcgttctca
    31321 ccaccgctgg acgaagaagg caacagtgtt cgcggtcaaa aaatggtgac atcggtcgct
    31381 aagcaactcg gctataacgt gtttaagggc tgatcatgat gaacacggaa ggtaataacg
    31441 gtaacaaacc tctcggtcta tggaatgtcg tttccatcgg tattggggca atggtggggg
    31501 cggggatctt cgcgctgctg gggcaggctg cgttgctaat ggaagcctcg acctgggtcg
    31561 cctttgcttt tggcggtatt gtggcgatgt tttccggtta tgcctatgcg cgcctggggg
    31621 cgagctatcc cagcaatggc ggtattatcg acttttttcg tcgcggatta ggcaacggcg
    31681 tcttttcgct ggcgctctcg ttgctgtacc tgttgacgct ggcggtgagc atcgccatgg
    31741 ttgcccgtgc ttttggcgct tatgccgtgc agtttttgca tgaaggcagc caggaggagc
    31801 accttatttt gctctacgcg ttggggatca ttgcggtgat gacgcttttc aactccttaa
    31861 gcaaccatgc ggtagggcgg ctggaagtga tcctcgtcgg cattaaaatg atgatcctgt
    31921 tattgctgat tattgccggt gtctggtcgc tgcaaccggc gcatatttcc gtctctgcgc
    31981 cccccagctc cggtgcgttc ttctcctgta tcgggataac cttccttgcc tatgcgggct
    32041 ttggcatgat ggcgaacgcg gcggataaag tgaaagatcc gcaggtcatt atgccacggg
    32101 cgtttctggt ggcgattggc gttaccacgt tgctttatat ctcgctggcg ctggttttgc
    32161 ttagcgatgt atcggcatta gagttagaaa aatatgccga taccgccgta gcgcaggcag
    32221 cttctccgct gctcgggcat gtgggttatg tgatcgtcgt catcggcgct ttactggcga
    32281 cggcttcagc catcaacgcg aacctgttcg ccgtgtttaa catcatggac aacatgggca
    32341 gcgaacgcga actgccgaag ctaatgaata aatccctgtg gcggcagagt acctggggca
    32401 acatcattgt cgtggtgttg attatgctga tgacggcggc attaaattta ggctcactcg
    32461 ccagcgttgc cagcgccacc tttttgattt gctatctggc ggtgtttgtg gtggcgatcc
    32521 gcctgcgaca tgatattcac gcctcgttgc cgattcttat cgtaggtacg ttggtgatgt
    32581 tgttggtgat cgttggcttt atctacagcc tgtggtccca gggcagccgt gcgttgatat
    32641 ggattattgg tgcaatttta ctcagcctta ttgtggcaat ggtcatgaag cgcaataaaa
    32701 ccgtataaca cctctctgcg cgcagtactg cctgtactgc gcctttgtct ttaattcaaa
//
LOCUS       DAPGOF010000039        32577 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.039, whole genome shotgun sequence.
ACCESSION   DAPGOF010000039 DAPGOF010000000
VERSION     DAPGOF010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.039"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(363..449)
                     /locus_tag="RHV13_001569"
     tRNA            complement(363..449)
                     /locus_tag="RHV13_001569"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(413..415),aa:Leu,seq:gag)
     gene            complement(464..796)
                     /gene="secG"
                     /locus_tag="RHV13_001570"
     CDS             complement(464..796)
                     /gene="secG"
                     /locus_tag="RHV13_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDV1241119.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(1024..2361)
                     /gene="glmM"
                     /locus_tag="RHV13_001571"
     CDS             complement(1024..2361)
                     /gene="glmM"
                     /locus_tag="RHV13_001571"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDV1241120.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(2354..3202)
                     /gene="folP"
                     /locus_tag="RHV13_001572"
     CDS             complement(2354..3202)
                     /gene="folP"
                     /locus_tag="RHV13_001572"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDV1241121.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(3292..5226)
                     /gene="ftsH"
                     /locus_tag="RHV13_001573"
     CDS             complement(3292..5226)
                     /gene="ftsH"
                     /locus_tag="RHV13_001573"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDV1241122.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(5326..5955)
                     /gene="rlmE"
                     /locus_tag="RHV13_001574"
     CDS             complement(5326..5955)
                     /gene="rlmE"
                     /locus_tag="RHV13_001574"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HDV1241123.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            6081..6374
                     /gene="yhbY"
                     /locus_tag="RHV13_001575"
     CDS             6081..6374
                     /gene="yhbY"
                     /locus_tag="RHV13_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDV1241124.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(6530..7006)
                     /gene="greA"
                     /locus_tag="RHV13_001576"
     CDS             complement(6530..7006)
                     /gene="greA"
                     /locus_tag="RHV13_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDV1241125.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            7254..8687
                     /gene="dacB"
                     /locus_tag="RHV13_001577"
     CDS             7254..8687
                     /gene="dacB"
                     /locus_tag="RHV13_001577"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HDV1241126.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(8727..9899)
                     /gene="cgtA"
                     /locus_tag="RHV13_001578"
     CDS             complement(8727..9899)
                     /gene="cgtA"
                     /locus_tag="RHV13_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HDV1241127.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(9915..10880)
                     /locus_tag="RHV13_001579"
     CDS             complement(9915..10880)
                     /locus_tag="RHV13_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDV1241128.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(11007..11264)
                     /gene="rpmA"
                     /locus_tag="RHV13_001580"
     CDS             complement(11007..11264)
                     /gene="rpmA"
                     /locus_tag="RHV13_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDV1241129.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(11285..11596)
                     /gene="rplU"
                     /locus_tag="RHV13_001581"
     CDS             complement(11285..11596)
                     /gene="rplU"
                     /locus_tag="RHV13_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDV1241130.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            11855..12826
                     /gene="ispB"
                     /locus_tag="RHV13_001582"
     CDS             11855..12826
                     /gene="ispB"
                     /locus_tag="RHV13_001582"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HDV1241131.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            13054..13332
                     /gene="sfsB"
                     /locus_tag="RHV13_001583"
     CDS             13054..13332
                     /gene="sfsB"
                     /locus_tag="RHV13_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HDV1241132.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(13380..14639)
                     /gene="murA"
                     /locus_tag="RHV13_001584"
     CDS             complement(13380..14639)
                     /gene="murA"
                     /locus_tag="RHV13_001584"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDV1241133.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(14694..14948)
                     /gene="ibaG"
                     /locus_tag="RHV13_001585"
     CDS             complement(14694..14948)
                     /gene="ibaG"
                     /locus_tag="RHV13_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HDV1241134.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(15108..15401)
                     /gene="mlaB"
                     /locus_tag="RHV13_001586"
     CDS             complement(15108..15401)
                     /gene="mlaB"
                     /locus_tag="RHV13_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HDV1241135.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGVTCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(15401..16036)
                     /gene="mlaC"
                     /locus_tag="RHV13_001587"
     CDS             complement(15401..16036)
                     /gene="mlaC"
                     /locus_tag="RHV13_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HDV1241136.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQRDAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(16055..16606)
                     /gene="mlaD"
                     /locus_tag="RHV13_001588"
     CDS             complement(16055..16606)
                     /gene="mlaD"
                     /locus_tag="RHV13_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HDV1241137.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(16611..17393)
                     /gene="mlaE"
                     /locus_tag="RHV13_001589"
     CDS             complement(16611..17393)
                     /gene="mlaE"
                     /locus_tag="RHV13_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HDV1241138.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(17401..18210)
                     /gene="mlaF"
                     /locus_tag="RHV13_001590"
     CDS             complement(17401..18210)
                     /gene="mlaF"
                     /locus_tag="RHV13_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HDV1241139.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            18420..19397
                     /locus_tag="RHV13_001591"
     CDS             18420..19397
                     /locus_tag="RHV13_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HDV1241140.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIIWLAMLYWLSPILVE"
     gene            19411..20397
                     /gene="kdsD"
                     /locus_tag="RHV13_001592"
     CDS             19411..20397
                     /gene="kdsD"
                     /locus_tag="RHV13_001592"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HDV1241141.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            20418..20984
                     /gene="kdsC"
                     /locus_tag="RHV13_001593"
     CDS             20418..20984
                     /gene="kdsC"
                     /locus_tag="RHV13_001593"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HDV1241142.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            20981..21556
                     /gene="lptC"
                     /locus_tag="RHV13_001594"
     CDS             20981..21556
                     /gene="lptC"
                     /locus_tag="RHV13_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HDV1241143.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            21525..22082
                     /gene="lptA"
                     /locus_tag="RHV13_001595"
     CDS             21525..22082
                     /gene="lptA"
                     /locus_tag="RHV13_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HDV1241144.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            22089..22814
                     /gene="lptB"
                     /locus_tag="RHV13_001596"
     CDS             22089..22814
                     /gene="lptB"
                     /locus_tag="RHV13_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HDV1241145.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            22862..24295
                     /gene="rpoN"
                     /locus_tag="RHV13_001597"
     CDS             22862..24295
                     /gene="rpoN"
                     /locus_tag="RHV13_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDV1241146.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            24318..24605
                     /gene="hpf"
                     /locus_tag="RHV13_001598"
     CDS             24318..24605
                     /gene="hpf"
                     /locus_tag="RHV13_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HDV1241147.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            24723..25214
                     /gene="ptsN"
                     /locus_tag="RHV13_001599"
     CDS             24723..25214
                     /gene="ptsN"
                     /locus_tag="RHV13_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HDV1241148.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            25260..26114
                     /gene="rapZ"
                     /locus_tag="RHV13_001600"
     CDS             25260..26114
                     /gene="rapZ"
                     /locus_tag="RHV13_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDV1241149.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            26111..26383
                     /gene="npr"
                     /locus_tag="RHV13_001601"
     CDS             26111..26383
                     /gene="npr"
                     /locus_tag="RHV13_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HDV1241150.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            26596..27228
                     /gene="yrbL"
                     /locus_tag="RHV13_001602"
     CDS             26596..27228
                     /gene="yrbL"
                     /locus_tag="RHV13_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HDV1241151.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDVAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALASREA"
     gene            complement(27225..27953)
                     /gene="mtgA"
                     /locus_tag="RHV13_001603"
     CDS             complement(27225..27953)
                     /gene="mtgA"
                     /locus_tag="RHV13_001603"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HDV1241152.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNEHNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSTPSGYMRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(27950..28603)
                     /gene="elbB"
                     /locus_tag="RHV13_001604"
     CDS             complement(27950..28603)
                     /gene="elbB"
                     /locus_tag="RHV13_001604"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HDV1241153.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGVGAAKNLS
                     NFASLGRECTVDRELKALAQAMHHAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(28833..31169)
                     /gene="arcB"
                     /locus_tag="RHV13_001605"
     CDS             complement(28833..31169)
                     /gene="arcB"
                     /locus_tag="RHV13_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HDV1241154.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(31265..32194)
                     /locus_tag="RHV13_001606"
     CDS             complement(31265..32194)
                     /locus_tag="RHV13_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDV1241155.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGSIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWIPPTE"
BASE COUNT         8095 a         8829 c         7857 g         7796 t
ORIGIN      
        1 acactgttag ttttttcttt ttaaatgatg cggccattgc gttctcagta attaaataac
       61 attatcaatg cgtctctaaa cattacgcag aacgtagcca tcagtagtta tactgacagg
      121 agcaggatgg tttatcgaga agtaaacata tccgtattcg taccggaact ggagttgaca
      181 caataaagtg ccaattatgt cagcagtagg gaaaagttta taacgaaggc gtaccggcac
      241 catcgtttca aggtaccagc tacgagtaaa gcaactggac gagatacaga tacctgacaa
      301 ccattcctca gaccaggacc aaaacgaaaa aagacgcttt tcagcgtctc ttttctggaa
      361 tttggtaccg aggacgggac ttgaacccgt aagccctatt gggcactacc acctcaaggt
      421 agcgtgtcta ccaattccac cacctcggca cggaaactac tttttagttc gggatatcgc
      481 tggtcggctt agccggagca gctggctgag tttgttcggt tttcgccggc gcactcaggt
      541 tttcccattc gctaccttta ttggttttgt tgctattgat gttacccagc accagactga
      601 tgatgaagaa taacgttgcc agcaacgccg tcatgcgggt catgaagtta ccagaaccac
      661 ttgaaccaaa cagcgtagcg gaagcgcctg ctccgaagga ggctcccata tcagcgcctt
      721 taccttgctg cagcatgatc agaccaacaa ggccaattgc cacaataagg aaaactacca
      781 aaagagcttc gtacataatc aacctgttcc ttgcggagtt gccgcgtacc aatgcttcaa
      841 ccaataaagc gggagttttt aatctttccc actgaagcgg gtgtgaatac taaccaaagc
      901 gaatgacctt cgcaagggca attttatcgc attgtatcaa ctgcggaaaa aaacagcaaa
      961 accgatgtgt tcgccgagaa aaaaggcgac aatcgccgcc tttttagcca gttatctaac
     1021 gctttaaacg gcttttactg catcggcgat gcggtgtgca aattcagtca cctgcgcttc
     1081 gtcttcgcct tccaccatca cgcgaattaa cggttcggtg ccggatttac gcaacagcac
     1141 acgtccacgg ctacccagcg ccgcttcaac ttctgcggtc acagctttga ctgattcatg
     1201 ctccagcgga tcgccgctac ctgcggtgta acgcacgttt accaggattt gcgggaacat
     1261 tttcatgccg ctgcaaaggt cgtgcaggct catatggtta cgcgccatcg ccgccagcac
     1321 ctgcaaacca gcaacgatgc cgtcaccggt agtagtttta tccagcagga tcacatgacc
     1381 ggaattctct gcaccgatac gccagccttt ctcctgcatt ttttccagta cgtagcggtc
     1441 acccactttc gcgcgcgcaa atggaatacc taactgtttc agcgccagtt caagccccat
     1501 gttgctcatc aatgtaccca cagcgccacc acgcagctgg ccctgacgaa gaccttcacg
     1561 cgcgatgata tacatgatct gatcgccatc gactttattg ccttcatggt caaccataat
     1621 cacgcgatcg ccgtcgccgt cgaaggcaat accgagatcc gctttttcag ccagcacacg
     1681 agcctggagc gcgcgaacgt cggtagcccc cacttcggca ttgatgttta caccgttcgg
     1741 ctcacaaccg atagcgataa cgttcgcccc gagttcacgc agcacgttcg gcgcgatgtg
     1801 ataagtcgca ccgtttgcac aatccaccac aatcttcagt tcactgaggc taagttcgtt
     1861 cgggaacgtg gctttgcaaa actcgatata gcgacccgcg gcatcaacga tacggctggc
     1921 tttacccagt tctgccgaat caacgcagct gatctccttt tccatttccg cttcgatggc
     1981 ctcttctacc gcatccggca gtttggtgcc gtcgatagag aagaatttaa tgccattatc
     2041 gtagaacggg ttatgcgatg cagatatcac aattccggcc tctgcgcgga aggtacgcgt
     2101 cagataagcc acggccggtg ttggcatcgg gccagtgaag agtgcggaaa ggcccgcagc
     2161 cgccagcccc gcttccagtg ctgactccag catatagcca gaaatacgcg tgtctttacc
     2221 aataataatc ttacgggagc cgtggcgcgc cagcacttta cccgcggccc aacccagctt
     2281 aagcacaaaa tcaggtgtga tcggcgcatc ccctacacga ccacgaatcc catcggtacc
     2341 gaaatattta cgattactca tagcgtttgt tttcctttgc agacagagtg gcttccacca
     2401 cccgcatcgc ttctacggtt tctttgacgt catgaacacg aatgatgtgc gcgccttgca
     2461 ttgcggcaat gaccgcacag gccagactac cgctcaggcg ctcggacggc cccacgttca
     2521 gcagctgccc aatcatcgat tttcgtgaca tacccaccaa cagcggcagg ttgaaatggt
     2581 gaaattcagc caggcgcgcc agtaatgaat agttatggga gagattttta ccgaaaccga
     2641 atccggggtc gagcagcaat ttctcttttg cgatacccgc ctgctcgcaa cgtgctattt
     2701 gctcaataaa gtagcgattc acttctgcaa agacatcgtc atacttcggg gcttcctgca
     2761 tggtttttgg atttccctgc atatgcatca gacaaaccgg taaaccggtt tctgcagccg
     2821 cctccagagc gccaggttcg gaaagggagc ggatatcgtt aataatgtga gcgccaactt
     2881 tcgctgactc acggatgact tctggtttgg atgtatcgac cgagatccag acttcgaagc
     2941 gttgagcaat tgcctcaacc acaggaataa cacgttgcaa ctcttcttca acgctaactt
     3001 ccgccgcccc tgggcgcgtg gactcgccac caacgtcaat gatcgtcgcg ccagcgttga
     3061 tcatcagatt cgcatgtttc accgcatcta tcagcgagtt atgcgtgcca ccatctgaaa
     3121 aggaatcagg cgtgacgttg aggatcccca ttacgtgagg atggctaagg tccagtgaag
     3181 taccctgggc aaagagtttc ataatgttat ccctggtatg aattgataag aaaaaacccc
     3241 ggagcacgcc ccggggtttt tggtacaaat acagtcatct tatgcgggaa cttacttgtc
     3301 gcctaactgc tctgacatgg tgttacccgg gttcggcgta cgcggttcat caaccggacg
     3361 aggagccttt ggactaccat tgtcgccaga attgttagaa gcgcctggtt cttcccagcc
     3421 cgctggcgga cgtacatcgc gacgtgccat caggtcatca atctgtggtg cgtcgatagt
     3481 ctcatatttc atgagagcat ctttcatcgc atgcagaata tccatattgt cggtcagaag
     3541 ctgacgcgca cgattatagt tacgctcaat cagtgctttc acttcctggt cgatgatacg
     3601 tgcagtttca tcggacatat gtttcgcttt cgctacgcta cggccgagga acacttcacc
     3661 ttcttcttcc gcgtacagca gtggacccaa tttctcagag aagccccact gagtcaccat
     3721 gttacgtgcc aggttggtcg caactttaat atcgttggac gcaccggtag aaacatgttc
     3781 cggcccgtag atgatctctt ctgccagacg accaccgtac agagtagaaa tctggctttc
     3841 cagtttctga cggctggcgc tgattgcgtc gccctcaggc aagaagaaag tcacacccag
     3901 cgcacgaccg cgtgggataa tcgtcacttt gtgcaccgga tcgtgttccg gcactaggcg
     3961 accgataatc gcatgacccg cttcgtggta agccgtcgat tctttctgcg cttccgtcat
     4021 caccatggag cgacgttccg cacccatcat gattttgtct ttcgctttct cgaattcaac
     4081 catcgacaca acgcgtttgt tgccacgagc agcgaacagt gccgcttcgt tcaccaggtt
     4141 cgccaggtca gcaccggaga aaccaggagt accacgggca atgattgccg cgtcgatatc
     4201 gggtgccaat ggtacgcgac gcatgtgaac cttcaggatc tgctcacgac cgcgaacatc
     4261 tggcaagcca accacaacct gacggtcgaa acggccagga cgcagcaggg ccgggtcgag
     4321 aacgtccgga cggttagtcg cggcgataac gatgatacct tcgttacctt cgaagccatc
     4381 catctcaacc agcatctggt tcagagtctg ttcacgttca tcgtgaccac cgcccagacc
     4441 tgcgccacgc tggcggccta cggcgtcgat ttcatcgata aagatgatgc acggtgccgc
     4501 tttcttcgcc tgttcgaaca tgtcacgaac acgggatgca cccacaccga cgaacatttc
     4561 tacgaagtca gaaccggaga tagtaaagaa cggaactttc gcttcgcctg caatcgcttt
     4621 cgccagcagc gttttaccgg tacccggagg accgaccatc aggacgcctt tcgggatctt
     4681 accgccgagt ttctggaagc ggctcggctc gcgcagatac tcaaccagtt cagcaacttc
     4741 ttcttttgct tcgtcgcagc ccgcaacgtc agcaaaggtc gttttgatct gatcttccgt
     4801 cagcatgcgc gctttgctct taccaaacga catggcacct ttgccaccgc cgccctgcat
     4861 ttgacgcatg aagaagatcc agacaccaat cagcaacagc atcgggaacc aggagatgaa
     4921 gatagaagcc agcaggcttg gttcttcagg cggttcaccg acaaccttga cgttcttggt
     4981 caacaggtta tccagtaatt tcggatcctg aaccggaatg taagtggtat aacggttact
     5041 atctttcttg gtaacgttga tttcacgtcc gttgatacgc gcttcacgaa cctggtcgtt
     5101 attgacctct tgtaggaagg tagagtaatc caccttacgg ccattagact cgctgggccc
     5161 aaagctctgg aatactgaca tcagcacaac ggcaatgacc agccagagta ttaggttttt
     5221 cgccatgtca ctcaagggat taacctctta ttacaactgt gttaaaaaca gcgtcaggat
     5281 actctatatc cagcatcttt caaactttcg tctgaaatct cccggttagg gtttacgccc
     5341 ggtcgctaca atatacactt cccgcgaacg tgcacgagaa gagtccggct tacgaacttt
     5401 gaccttcgta aacagggagc gaatttccct tagatactca tcgaaacctt cgccctggaa
     5461 caccttcact acaaaactgc cacctggcgc taatacatca cgacacattt ctagcgccag
     5521 ttccaccaga tacatggcac gggggatatc caccgccggt gttccgctca tgtttggtgc
     5581 catatcggac atgacaacct ggactttgct gtcgccaacg cgctccagca gtgctttcat
     5641 caccagttca tcacgaaaat cgccctgaag aaagtccaca ccaacgatag gatccatagg
     5701 taaaagatcg caagcgatga tgcggccttt gccgccaatt tgggtgacca catattgtga
     5761 ccaaccaccc ggagcagcac caaggtcgac aaccgtcatt cccggtttaa agagtttgtc
     5821 actttgctgt atttcatcaa gtttaaacca ggcacgggaa cgtaacccct ttttctgtgc
     5881 ctgttgaaca tatttatcgc taaagtgttc ctgaagccag cggctggagc tggcagaacg
     5941 cttcttacct gtcatttaac tttcccatgg ggataactca tcgtaaccaa ttgcgtaaat
     6001 ttttacgcgc ctatttggtg atatatggga gatggcggta gaatgacccg ttttcaatcc
     6061 caacgtaagc aaaaaatacg atgaatctga gtactaaaca aaaacagcac ctgaaaggtc
     6121 tggcacatcc gctcaagcca gttgttctgc ttggcagtaa tggtttgacc gaaggggtgc
     6181 tggccgagat tgaacaagcg ttagagcacc atgaactcat caaggtgaaa atcgccaccg
     6241 aagatcgcga aacaaaaacc ttgatcgtgg aagctatcgt gcgcgaaacc ggcgcctgta
     6301 atgtacaggt catcggtaaa acgctggtgc tttatcgccc aactaaagaa cgtaaaatct
     6361 cgctgccacg ctaagattat cctaaagtta cacacatttg ctgtgtaaaa cgaggggttt
     6421 tccgcaggca ggagagcaaa atgccacgct ctgttcgttg ataaaaggcc gcatagcggc
     6481 ctttttcctt tctttacaat acatcaacat cttgagtatt gggtaattct taaaggtatt
     6541 ccaccttaat tacttcaaat tctacttcgc cgcccggcgt tttgatgacc acaacatcat
     6601 cttcttcttt gccgatcagg ccacgagcaa taggcgagtt tacagaaatc aggttttgtt
     6661 taaagtcagc ttcgtcatcg ccaacgatgc gataagtctg ttcttcgtca gaatcgagat
     6721 tcagcaccgt tacggtagca ccaaaaataa cgcgcccatt gttgggcatt ttggtgacat
     6781 caatcacctg cgcgttcgac agcttggctt cgatgtcttt gatacggcct tcgcagaagc
     6841 cctgctgttc acgagctgcg tggtattcgg cgttttcttt caggtcgcca tgctcacgcg
     6901 cttccgcgat agcagcaatg atttcaggac ggcgcacaga tttcagaaaa tccagctctt
     6961 cgcgtaattt ttcagcgccg cgtaaggtca tcggaatagc ttgcatttgt tatacctctt
     7021 gaatattcct gatggggcaa gttttcaccc accccggctg ttcggcctgc ccggcataac
     7081 tcctcaccgg gaccagaagc aaaaaaatac cgacccgggt acaagtccca ggtcagctac
     7141 aattcacatt ttgatagtca ttttaccctg aagttcccga agggtcatcg tttactttat
     7201 agggcgttgc gccgtagtat gacggctcga ttccaggttg ttagcgcgag attatgcgat
     7261 tttccagatt tatcatcgga ttgaccagct gtatagcgtt cagtgttcag gccgcaaatg
     7321 ttgatgagta cattactcaa ctccccgctg gtgccaacct tgccctgatg gtgcaaaaag
     7381 tcggcgcgtc ggcccccgct attgattacc acagtcagca gatggcgctg cccgccagta
     7441 cccagaaagt gattactgcg ctggcggcgt tgattcaact cggccccgat tttcgtttca
     7501 ccacgacgct tgaaaccaaa ggcaatgtgg aaaacggcgt acttaagggt gacttagtgg
     7561 cgcgatttgg tgccgatccg acgttaaaac gtcaagatat tcgcaatatg gtcgcgactt
     7621 tgaaaaaatc tggcgtcaac caaatcgatg gcaatgtgtt gatagatacc tccattttcg
     7681 ccagccacga taaagccccc ggctggccat ggaatgacat gacacaatgc tttagcgctc
     7741 cgcctgccgc cgccatagtt gaccgcaact gtttctccgt ctcgctctac agtgccccaa
     7801 aacctggtga tatggctttt atacgcgtgg catcttatta ccccgttacg atgttcagcc
     7861 aggtacgcac cctcccccgt ggttctgccg aagcgcaata ctgcgaactg gatgtcgtac
     7921 caggcgacct gaaccgcttt acgctaacgg gatgcctgcc gcaacgttct gagccgctcc
     7981 cgttggcttt tgccgtgcag gatggagcca gctatgccgg ggcaattctg aaagatgagt
     8041 taaaacaggc aggtatcacc tggagcggaa cactgctgcg ccagactcag gttaacgaac
     8101 ctggaacggt agttgccagt aaacagtcgg ccccgttgca cgatctgctt aagattatgc
     8161 tgaaaaagtc ggacaacatg atcgccgata cggttttccg catgataggc catgcgcgct
     8221 tcaatgtgcc tggaacatgg cgggccgggt cggacgctgt gcgtcagatc ctgcgccagc
     8281 aagccggtgt cgatattgga aataccatta ttgccgatgg ttcagggctt tcgcggcata
     8341 acctgattgc ccccgccacc atgatgcagg tgctgcaata cattgcccaa cacgacaatg
     8401 aacttaactt tatctccatg ctgccgctgg cgggctatga cggctctttg cagtaccgtg
     8461 caggtctgca tcaggcgggc gtggatggca aagtctcagc gaaaaccggt tcgttgcagg
     8521 gggtatataa cctggcggga ttcattacca cagcgagcgg gcaacgaatg gcgtttgtac
     8581 aatatctttc tggctatgca gtagaaccgg cggatcagcg taatcgccgt attccgttag
     8641 tgcgttttga aagccgtttg tataaagata tttatcagaa caattagtca aaaagaaacc
     8701 ccggcacatg gccggggttt tgctgattaa cgcttgtaaa taaactcaac gccttcttcg
     8761 tcgtcttcgt cccaatcgtc atcccagtct tcatcatcct cttcagcaat ctcttcaagc
     8821 tgctggcgat gataatcatc ccacatgaat tcgactttct ctggctgttt cgcttcttca
     8881 gcctgcacga ccgggttttc aatgataaag gtcatcacat cccagcagag atctttcacg
     8941 cccagtccgc tcgccgcaga gatcagataa tatttatctt cccagcccag cgcttcagcg
     9001 atcgctttcg ctttctcttc ggcttctgcc ttatccagca gatcgatctt attgaacact
     9061 aaccaacgcg gtttcgccgc cagatcctgg ctgtattttt ccagctcgct gataataata
     9121 cgcgcgtttt caaccggatc ggtgccgtca atcggatcga tatcgatgag gtgcaacagg
     9181 acgcggcaac gttccaggtg cttcaggaag cgaatgccca gacctgcgcc ttccgcagcg
     9241 ccttcaatca gtcctggaat atcggcaaca acgaagctct tttcgttgtc cattcgtacc
     9301 acacccagac ttggcaccag agtggtaaac ggataatccg ccactttcgg tttagccgcc
     9361 gataccgcac gaataaaggt cgatttaccc gcgtttggca tccccaacat accgacgtca
     9421 gccagcagca tcagctccag cagcagctca cgcttatcgc ccggcgtacc gttagttttc
     9481 tgccgtggtg tacggttaac tgacgatttg aaacgggtat tgcccagacc gtgccagccg
     9541 cccttagcaa ccagcagacg ctgaccgtgt ttggtcatat cgcccatagt ttcaccagta
     9601 ccctggtcga ttacacgcgt acctaccggc actttaatcg tgacgtcttt accgcgctta
     9661 ccggtacagt cgcggcttgc gccgttctga ccgcgctctg cacggaaaga tttttcaaaa
     9721 cgataatcga taagcgtgtt caggttctcg tcggcttcca tccatacgtc accaccatca
     9781 ccgccatcgc cgccatccgg gccgcctttc ggaatatact tttcgcggcg gaagctcacg
     9841 caaccattac cgccatcacc tgcaacgacc agaatcgatg cttcatcaac aaacttcatt
     9901 ttattctccg taaatcattc gcctgcgcgg ggttgcgata ccaccgttgt atgcttacgt
     9961 aatccgcccc aaatacgatg accaatggcg gaatacatcg cgcccgcaac cacgacaaac
    10021 gcaccgagat aacctaaaag gtttaacatc ggtctggcga agaaatcggg ccaggccagt
    10081 gataaaagat ctgaaaaaaa cagcgtaaac agtggggtga gcgtgatgat cgcgctcacc
    10141 tgcgctgcct gccagcgagc catcgcttcc gccagggcgc catatcctac caaggtattc
    10201 agtccgcaaa aaattaaaca tgcgagctgc cagtggctaa gctgcgctat cactccaggc
    10261 tttgccagag ggaagagcgc aattgtacat aaagtgtaca ataaaaacag gatctgcggt
    10321 gaggccagcc gacgcaataa aaccttttgc gccacgccat aactcaccca aaccgtcgcc
    10381 gcaccgaccc caaagataac tccccaggtg taatcggtga gctttgtaaa tatctcgacc
    10441 agactggtgt taaaaaacat caccaggccg ctcaggagca tcaatgcccc tacaacctga
    10501 gtgctgcgca ttttctcttt caggataaat acgctggcaa ccatcatgcc aactggcgag
    10561 agttgcccaa tcacctgcga agcggtcgga ctcaggtatt gcaaggatga gctgaacagg
    10621 atgaagttcc caaacagccc ggcggtcgcc actgccaaca aaatcaacca gcgtggctta
    10681 cgaaacacgc gtaatggcgg caacctcttc ttcaccgcaa gaatggcacc caggccaata
    10741 ctcgccatca agaaacggta aaacacgatt gtcggaggtt ccatcacctc cagcacctgc
    10801 ttcattgcga ttggcaacgc cccccagcaa attgctgtgg tgagcgccaa aagaatgcca
    10861 atgcctgcct gctgcttcat accgtattcc ctgcaaagcg catttgcgga ttaacgtcgc
    10921 accatgcgca tttttaccgg ttatcgaatg taaaaagccc cgcaacgtgt tgcggggctt
    10981 tcatccgtta ccgggacgcg aaaaacttat tcagcttcga tgctgataaa tttacggttt
    11041 ttcgggcctt taacttcgaa tttcacttta ccgtctgctt tagcaaacag agtgtggtca
    11101 cgaccgcaac ctacgttagc gccagcgtgg aatttggtac cacgttgacg aacgatgatg
    11161 ctacccgcca gaacggattc gccaccgaaa cgcttaacgc ccaggcgttt agcttctgaa
    11221 tcgcgaccgt tacgtgtgga gccgccagcc tttttatgtg ccatttgaaa tctctcctca
    11281 ggtcttaggc gctgatgcca gtaattttca catcagtgaa ccactgacga tggccctgct
    11341 gcttacgata gtgtttacga cgacgaaact taacgatttt aactttctcg ccacgaccgt
    11401 gagcaacaac ttcagctttg attacgccgc catcaacgaa aggaacgccg attttgactt
    11461 cttcaccgtt tgcgatcatc agcacttcag cgaactcaac agtttcgcca gttgcgatgt
    11521 ccagcttttc caggcgaacg gtctgacctt cgcttactcg gtgttgttta ccaccacttt
    11581 ggaaaaccgc gtacatataa aactccgctt ccgcgcacac cttttcaatg attcagagtg
    11641 cgctataaat attcacaata gggcgcgaat attacgcaaa acgcacgcct ttgacaagtg
    11701 ctacagtcaa tacacgaaga aaaaaaacac aacttgtacg gtaacgttta tctgtgccat
    11761 tttttcagta caatcaccct atattcctaa ccataaaccc taagttgcct ttgttcacag
    11821 taaggtaatc ggggcgaaaa gcccggcttt tgcgatgaat ttagaaaaaa tcaatgagtt
    11881 aaccgcgcaa gatatggcgg gtgttaatgc ggcaatcctt gagcagctta attccgacgt
    11941 ccaactgatc aatcagttag gctattacat cgtcagcggc ggcggtaaac gtattcgtcc
    12001 gatgattgct gtactggctg cacgagctgt tggctatgag ggaaatgcgc atgtcaccat
    12061 tgctgccctg atcgagttta tccacacggc gactctgcta cacgacgacg ttgtggatga
    12121 atcagatatg cgcaggggta aagctaccgc caacgccgca tttggcaatg ccgccagcgt
    12181 gctggtaggc gattttattt atacccgcgc tttccagatg atgaccagcc tcggttcgct
    12241 caaagtgctg gaagtcatgt cagaagccgt aaacgtcatc gcagaaggtg aagttctgca
    12301 actgatgaac gttaacgatc cggacatcac tgaagaaaac tacatgcgcg ttatctatag
    12361 caaaaccgcg cgtctgtttg aggctgccgc gcagtgttcc gggattctgg ctggctgtac
    12421 gccggaggag gagaaaggcc tgcaggatta tgggcgctat ctcggcactg ctttccagtt
    12481 gatcgacgat ttactcgatt acaatgccga tggcgaacag ttaggtaaaa acgtcggcga
    12541 cgatctgaac gaaggtaaac cgacgctgcc gctgctgcat gcgatgcatc atggtacacc
    12601 agaacaggca cagatgatcc gtactgccat cgaacagggt aacggtcgcc atcttctgga
    12661 accggttctg gaagcaatga acgcttgtgg atctcttgaa tggacgcgtc agcgcgccga
    12721 ggaagaagca gacaaagcca tcgcagcgtt acaggtgctc ccggacaccc cttggcgaga
    12781 agcactcatc ggcctcgcgc acatcgctgt tcaacgcgat cgttaatccc ctcccctcat
    12841 cccgcgcagt gagcgggatg attccagtaa actccataag aatacttatt cagtgtaata
    12901 atgcctgaaa aaccagtgta tacatcttta tatattctga atattcacaa tctttacagg
    12961 aactttttag agcaatcggc catcaggagt atagtgatgc tcgacagaag aagtgttctg
    13021 aatgaaagcg actaacttaa ggagtgagga aagatggaaa gtaatttcat tgactggcat
    13081 cccgctgaca tcattgcggg tttgcgcaag aagggaacgt caatggcggc ggaatctcgc
    13141 agaaatggtt tgagttcctc aacgctggca aacgcattat cgcgcccatg gccgaaagga
    13201 gagatgatta ttgcgaaagc cctgggaact gacccctggg ttatctggcc atcacgctac
    13261 catgatccgc agacccatga gtttatcgac agaacgcagt tgatgcgtag ctacactaaa
    13321 ccgaaaaaat gagtggtctg gcggtagccc cgcgaacggg gctgccagct ctcagacgat
    13381 tattcgcctt tcacacgctc aatatttgca cctaaagcgc gcagtttgtc ttcaatgcgt
    13441 tcgtagccac gatcgatgtg ataaatacga tcaaccaccg tcgtcccttc cgcaatacag
    13501 ccagccagca ccaggcttgc tgatgcacgc agatcggttg ccataacctg tgcgccagaa
    13561 agtttttcaa caccgtgaca aataacggta ttgctttcga tttcggcgtg cgcgcccata
    13621 cggctcagct ctggcacatg cataaagcgg ttttcaaaga ccgtttcggt gatgaatcca
    13681 gtcccttctg ccaccaggtt caacagcgtg aactgggcct gcatatcggt cgggaatgcc
    13741 ggatgcggcg cggtacgtac gttaacagcc ttcggacgtt tgccatgcat atccaggcta
    13801 atccagtctt cgccgacttc gatgtccgct ccagcatcac gcagtttcgc cagcacggcg
    13861 tcgagagtat ctggctgcgc gttacggcag ataattttgc cgcgagaaat cgccgccgcc
    13921 accaggaaag taccggtttc gatacgatcc ggtagcacgc gatagacacc gccgcctaaa
    13981 cgttccacac cttcgatgac gatacgatcg gtgccctgac cgctaatttt cgcacccagc
    14041 gtaatcagga agttcgcggt atcgacgatt tccggttcac gcgctgcgtt ttcaataatc
    14101 gtggtgcctt ccgccagggt tgcagcacac atgatggtca ccgttgcgcc aacgctgact
    14161 ttatccatca cgatatgtgc gcctttcaga cgaccatcga cggaagcttt aacgtaacct
    14221 tcttccagtt tgatggtcgc gcctaattgt tcgaggccag aaatgtgtag atcaaccgga
    14281 cgcgcaccga tcgtacaacc gccaggtagt gaaacttgcc cctgaccaaa gcgcgctacc
    14341 agcggcccca gtgcccagat agaagcacgc atggttttaa ccagatcgta aggtgcgcag
    14401 aatacattaa cgtcgcgggc atcaatatgc acagaaccat tacgttctac tttcgcaccc
    14461 agctggctta gcagcttcat tgatgtatcg acgtctttca gtttcgggac gttctggatc
    14521 tctaccggtt cttccgccag tagtgcggca aaaaggatag gcagagcagc atttttagcg
    14581 ccggaaattg tgacttcgcc ctggagcttc gttggcccct gaacacgaaa tttatccatt
    14641 tagtttgttc tcagttaaca attcatatcc gctaccggcg aatcgcccat agctcaaaag
    14701 ccgttcagtt tgcgatcgcg cgcccactca gcaggggtat acgctttgat cgacacagca
    14761 tgaatgcggt tatccgcaat atattccatc agcggaccat agaccgtctg ctgtttttta
    14821 acccgactca tgccgtcaaa caactcaccc acggcaataa cctgaaagtg gctgccatcg
    14881 ccggaaacgt ggacttcctg gagggagaga gcgttcatca acacgctctg aatttcatta
    14941 ttttccatgg gatcttcaat catcagttaa taaaccagcg aaacatctta gagcaaagtt
    15001 gcgctggcat aaataagcaa aaagcctcgc tgataaatca gacaaggctc gacttgcagg
    15061 caggtttgcc ggacaggcgg ttaacgccat atccggcctg aaaaaattta acgaggcaga
    15121 acatcagcag gcaaattata caatttcgcc agggtataca ctttgtcgtt taccccctga
    15181 agcgtcacat tgttgccctg ctttttcgcc agatcgataa gatggagcag cagtgccagt
    15241 ccccccgtat ccacgcggga cacacggcta agatcgatgc aggtaacccc cttcaccgct
    15301 tcctcacgca tttcccaaag cggtagcaga acgtcctgat ccagctctcc ggataacgcc
    15361 agcgtgtcac ccgtctgcat ccagctcagt gactcgctca ttattttttc tcttccagag
    15421 tgattttctg ttgagaaatc gatttcagtt gcgcagtcag gccgtcgata cctttggtac
    15481 gcagcagcgt tccccactcg ttttgtttgg tggtgatcat actgacgcct tcagcaatca
    15541 tgtcgtaagc ctgccagttg cccgtctggg agtttttacg ccactggaag tccagacgca
    15601 ccggcgggcg accattcggg tcaataatgg taacgcgaat aggcacaatg gttttatcac
    15661 ccagcggctg ttctggcgca atctgatagg tttgaccgtg atacatcgcc agcgcctgac
    15721 cataagcctg cttcaggtac tcacggaaag cggcaaagta agcatcacgt tgagctgggg
    15781 tcgcactctt gtaatactgg cccagcacca gcgcaccggc gtatttcacc tgtacgtatg
    15841 gcaacagttc ctgatcaaca atggtacgca gataatccgg gttggcccga atttgcggtt
    15901 gctcattctt caggcgatcg aacgttttct gcgccgcctc gtccatcagc ttatacggat
    15961 tggtctggtc tgccgcggtt gccgcactca gcggtgcaat caccagcaaa gcgaccatca
    16021 ttaaacgttt aaacatgcgt cggttctcct gaaattattt cgttgtaccc acaggttcag
    16081 tggtttcatt attacctggc gcagcagctg gcgcatcgcc actattctta ttgtcatcgc
    16141 ctttactacc gtaaaggaac tgaccaatga gatcttccag caccatcgca gacttggtgt
    16201 cctgaattgt atcgccatcc ttcaggatag cagtccccag ttccgggtct tcaaaaccga
    16261 cgtttaatgc cagatattgt tcccccagca ggccggaagt acgaatgcta agcgaactgg
    16321 tatcaggaat gtggttataa cgttgttcaa tttccagcgt tacgcgcggc agataggttt
    16381 tcgggtccag cgtaatatcc gccacccggc ccacaacaac gccaccaatg ctgaccggag
    16441 agcgcgcttt caggccgcca atgttatcga acgtcgcata aagcgtgtag gtcggttcag
    16501 tacgtattga cgtcacgttc gccgccttca ggcaaacaaa cagcgccgcc agcagtgctg
    16561 ctaataaaaa gatacccacc caaatttcat tttttttcgt ttgcatgaac tcaattccca
    16621 aacatcaatg cggtcagcac aaaatccagc cccagaacag ccagagacga gtggacaacg
    16681 gtgcgagtgg ttgcccggct aatcccggca gacgtcggga tggcgtcgta gccgttaaac
    16741 aacgaaatcc acgtcaccgt gatggcgaac actacgctct taatcagaca gttgaccaga
    16801 tccatacgcc agtcgacggc attttgcatt gccgaccaga agaacccgct atcaatgcct
    16861 ttccaactga cgccgaccag cgatccgccc cagatcccca cggcgacgaa aataaccgtc
    16921 aacagtggta atgaaataac cccagcccag aaacggggag aaataacccg acgcagcgga
    16981 tccaccgcca tcatctccat actggagagt tgctctgtag cgcgcatcag gccgatttct
    17041 gcggttaacg ccgaaccagc acgcccggca aacaacagcg cggcaaccac cggccccagt
    17101 tcacgcagta gcgataacgc caccagcata cccagactgg tttccgcact ataagtggtc
    17161 agaaccagat aaccttgcag ccccaacacc attccgataa acacgccaga aactacaata
    17221 atcagcatcg acaggacgcc gacattatag agctggcgca ccagcagcgg cgcatgtttg
    17281 cgaaattccg gtttgccgac cagcgcattg aataacatta acccggcccg cccgaacgtt
    17341 ctcagggttt taatcccttt gtgtccgagc gacgccagcg catttaacag catgagtggc
    17401 ttaactccct ggtaaaagat cagcgtgata atcgccggca ggatagcgga acggaacagg
    17461 cccgtcagct atcccgtcca gaaactgacg tacgcgcgga tcaggattcg cctgcaacgc
    17521 ctgggcactg ccatgagcga caattttttt gtccgccagg atccaggcgt gatccgcaat
    17581 acttaacact tccggcacat cgtgagaaac caccacacaa gtcacgccca gcgcgctgtt
    17641 cagctcagaa atcagcttca ccagtacgcc catggtgatg ggatcttgcc caacaaaggg
    17701 ttcatcaaac atgatgagat ccggctccag cgcaatcgca cgcgccagcg ctgcacgccg
    17761 cgccatccca ccggaaagtt cagaaggcat tagcttagcc gctccacgca gccccacggc
    17821 ctccagcttc atcatcaccg tactatgcaa caacggcgcg ggaagttggg tatgttcgcg
    17881 cagtggatag gcgacgttgt caaatacgtt catatcagtg aacaacgccc cggactgaaa
    17941 taacatgctc atccgtttgc gcactgtata caggcgcgaa cgagacatcg ccggaatatt
    18001 ctcaccatcg aaaaggatct caccatgatc tggtgcgatt tgcccgccaa tcagacggag
    18061 tagcgtcgtt ttaccgatgc ccgatggccc catgatcgcc gtgatcttcc ctcgcggcac
    18121 ggtcagggaa atattatcga agatgcagcg attgccacgc gtaaaactga catcgcgcat
    18181 atcgactaaa ttcgccacag actgctccat aattcaccct tcgtcttgcg ttgattttct
    18241 aagcatggcg ctcaatttaa ccttgaaccc aacatattta cagaatatta cccgccgtgg
    18301 ttagcgaaag ctggcatttg ttttactttt tagccgcata aagtcaaaat taagaatccg
    18361 ttacggcttt ctgaaaatct tcagcggacc ggcgagtata cctgaagaaa ggacgttaga
    18421 tgcttttagc tacggcactg ttaattgttg gtttactttt ggtcgtttac agtgccgacc
    18481 gcctggtttt tgccgcgtct attctttgcc gaacctttgg catcccgccg ctgatcatcg
    18541 gcatgacggt ggtcagtatt ggtacatcgt taccagaaat catcgtctcg cttgccgcgt
    18601 ctctgcacga acaacgcgat ttagccgttg gtacagccct cggctcaaac attatcaata
    18661 ttttgctgat cctcggtctg gctgcgctgg ttcgtccttt taccgtccat tctgatgttc
    18721 tacgccgtga attaccctta atgttgttgg tcagcgtggt ggccggttcc gtactctatg
    18781 acggacaact tagtcgcagc gatggtatct ttctcctctt tctggctgtg ctatggctgc
    18841 tgttcattgt taaacttgca cgtcaggctg aacgtcaggg gactgacagc ctgaccagag
    18901 agcagcttgc agagctgccg cgtgacggcg gattgcccgt cgcgttttta tggctcggca
    18961 ttgcgcttat catcatgcca gtggccacgc ggatggtggt tgataacgcc acggtgctgg
    19021 cgaattactt tgccatcagc gagttgacga tgggcctgac ggcaattgct atcggaacca
    19081 gcctgccgga actggcaacc gcaatcgcgg gggttcgcaa aggtgaaaac gacattgctg
    19141 tcggaaatat cattggcgca aacattttta atattgtcat cgtgttgggt ttacccgcgc
    19201 tgataacgcc aggagagatt gatccactgg cgtacagtcg tgactacagc gtgatgttac
    19261 tggtgagtat tatttttgcg ttgctgtgct ggcggcgttc cccgcaaccg ggccgtggtg
    19321 taggggtatt attaactggc ggatttatca tatggctggc gatgttgtac tggttatcgc
    19381 caatactcgt tgaataactg gaaacgcatt atgtcgcacg tagagttaca accgggtttt
    19441 gactttcagc aagcaggtaa agaagtcctg gcgattgaac gtgaatgcct ggcggagctt
    19501 gatcaataca tcaatcagaa tttcacgctt gcttgtgaaa agatgttctg gtgtaaaggg
    19561 aaagttgtcg tcatggggat ggggaaatcg gggcacatcg ggcgcaaaat ggccgcaacg
    19621 tttgccagca ccggtacacc ttcatttttc gtccatcctg gtgaagccgc gcatggtgat
    19681 ttaggcatgg tcaccccaca ggatgtggta attgctatct ctaactcagg tgaatccagc
    19741 gaaatcacgg ccttaattcc agtgcttaag cgtcttcacg taccgttaat ctgcatcacc
    19801 ggtcgcccgg agagcagcat ggcgcgcgcc gcagatgtgc atctgtgtgt taaagtagcg
    19861 aaagaagcct gtccgttagg gctggcaccg accagcagca ccaccgccac gctggttatg
    19921 ggcgatgccc tcgctgtcgc gctgttaaaa gcacgcggct ttactgctga agattttgcg
    19981 ctctcacacc caggcggcgc actgggtcgt aaacttctgc tgcgcgtaaa cgatattatg
    20041 catacgggcg atgagatccc gcatgttaag aaaacggcca gtctgcgtga cgcgttactg
    20101 gaagttaccc gcaaaaatct tggtatgact gtcatttgcg atgacaatat gatgattgaa
    20161 ggcatcttta ccgacggtga tttacgccgt gtcttcgata tgggcgtgga tgttcgtcag
    20221 ttaagtattg ccgatgtgat gacgccgggg ggaatacgcg tgcgccctgg cattctggcc
    20281 gttgaggcac tgaacttaat gcagtcccgc catatcacct ccgtgatggt tgccgatggc
    20341 gaccatttac tcggtgtgtt acatatgcat gatttactgc gtgcaggcgt agtgtaaaga
    20401 ttcaaggata aacaacaatg agcaaagcag gtgcgtcgct tgcgacctgt tacggccctg
    20461 tcagcgccga cgttatagca aaagcagaga acattcgtct gctaatcctc gacgttgatg
    20521 gcgtactgtc agatggcctg atttatatgg gcaataacgg cgaagagctg aaagcgttca
    20581 atgttcgtga cggttatggc attcgttgtg cgctcacctc tgatattgaa gtcgctatca
    20641 ttaccgggcg aaaggctaaa ctggtagaag atcgttgtgc cacattgggg atcactcact
    20701 tgtatcaggg gcagtcaaac aaactgatcg cctttagcga tctgctggaa aaattggcga
    20761 ttgccccgga aaatgtggct tatgtcggcg atgatctcat cgactggccg gtaatggaaa
    20821 aagtgggttt aagcgtcgcc gtggccgatg cgcatccact gttgatcccg cgcgccgatt
    20881 acgtgacgcg cattgctggc ggtcgtggcg cagtgcgcga agtttgcgac ttattactcc
    20941 tggcgcaggg caaactggat gaagccaaag ggcaatcgat atgagtaaag ccagacgttg
    21001 ggttatcatt gtgctatcac tggcggttct ggtgatgatc ggcattaata tggccgaaaa
    21061 agacgatacc gcccaggtgg tcgtcaacaa caatgatccc acctataaaa gcgagcatac
    21121 ggacacgctc gtctataacc cagaaggggc gctaagctat cgattgattg ctcaacacgt
    21181 tgaatattat tccgatcagg ccgtttcgtg gtttacgcag ccggtactta ccacgtttga
    21241 taaggataaa atcccgacat ggtccgtaaa agcagataaa gccaagctga ccaacgaccg
    21301 gatgctctat ttatatggac acgttgaagt caacgcactc gtgccggact ctcaacttcg
    21361 cagaatcacg acggataacg cgcagatcaa tctggtgacg caggatgtta cctctgaaga
    21421 cctcgtcacg ttatacggaa caacatttaa ctccagcggt ctgaaaatgc gcggcaactt
    21481 acgcagcaag aacgccgagc tgattgaaaa ggttagaaca tcctatgaaa ttcaaaacaa
    21541 acaaactcag ccttaatctt gtgcttgcca gctcacttct ggccgccagc attccggcat
    21601 ttgccgtaac cggagacact gatcagccga tccacattga atcggaccag caatctcttg
    21661 atatgcaagg caacgtggtt acctttaccg gtaatgtcat cgtcacccag ggcaccatca
    21721 aaattaatgc cgacaaagtg gtcgttaccc gtccgggtgg cgaacaaggt aaagaagtga
    21781 ttgacggcta cggtaaaccg gcaacgttct accagatgca ggacaacggt aaacccgttg
    21841 aaggtcacgc ttcccagatg cactacgaac tggcaaaaga ttttgtcgtt ctgacgggta
    21901 atgcttatct gcagcaggtc gatagcaaca ttaagggcga taagatcact tacctggtga
    21961 aagagcagaa aatgcaggct ttcagcgaca aaggcaagcg cgtaaccacc gttctggtgc
    22021 cgtcgcagct gcaggacaaa aacaacaaag gccagacccc ggcacagaag aagggtaatt
    22081 aattcgttat ggcaacatta actgcaaaga accttgcaaa agcctataaa ggccgtcgcg
    22141 tggtagaaga cgtcagcctg accgtcaact ccggggaaat tgtcggtctg ctggggccaa
    22201 acggtgccgg taagaccacc actttctata tggttgttgg cattgtgccg cgcgatgcgg
    22261 gcaacatcat tattgatgat gacgatatca gtctgctgcc tctgcatgca cgcgcgcgcc
    22321 gcggtatcgg ctatctgccg caggaagcct ccattttccg tcgcctcagc gtttacgata
    22381 acctgatggc ggtactgcaa attcgtgacg acttgtctgc tgaacaacgt gaagaccgcg
    22441 cgaacgagct gatggaagag tttcacattg agcacctgcg tgacagcatg gggcagtcac
    22501 tctccggggg tgaacgtcgc cgtgtagaaa ttgcccgcgc actggctgcg aatccgaaat
    22561 ttattctgct cgacgaaccg tttgccgggg ttgacccgat ctcggttatc gacattaaac
    22621 gcatcattga gcacctgcgc gacagcggcc tgggcgtgct gatcactgac cacaacgtgc
    22681 gtgaaacact ggcggtttgt gaacgcgctt atatcgtcag tcaggggcat ttgatcgccc
    22741 acggcacgcc tacagaaatc ttacaagacg aacacgttaa gcgtgtatac cttggggaag
    22801 acttcagact ctgatagggt agaagtttgc gacgttttag caggagagta cgattctgaa
    22861 catgaagcaa ggtttgcaac tcaggcttag ccaacaactg gcgatgacgc cacagcttca
    22921 acaggcaatt cgtctgttgc agttgtcgac gctggaactt cagcaggagc tacagcaggc
    22981 gctggagagt aatccgctgc ttgagcaaat cgacactcat gaagaaatcg acacccgcga
    23041 aacgcaagac agtgaaacgc tggacaccgc cgacgcgctc gaacaaaaag agatgccgga
    23101 agagctgccg ctcgatgcca gttgggacac catttacacc gctggtacac catccggcac
    23161 cagcggtgac tacattgacg acgagctacc ggtctatcag ggcgaaacga cgcagacctt
    23221 gcaggattac ctgatgtggc aggtcgagct gacaccgttt tccgacactg accgcgctat
    23281 tgctacctct atcgtcgatg ccgttgatga caccggttat ctgactgtcc cgctggaaga
    23341 tattctcgaa agtatgggcg atgaagagat cgacatcgac gaggttgaag ccgtccttaa
    23401 gcggatccaa cggtttgatc cggtcggtgt agcggcaaaa gatctgcgtg actgcctgct
    23461 aatccaactc tcccaattcg ataaaaccac gccgtggctg gaagaggcca gactgatcat
    23521 tagcgatcat ctcgatctgt tagccaatca cgacttccgc actttaatgc gcgtcacgcg
    23581 tctgaaagaa gatgtgctga aagaagccgt caatctgatc cagtcgctcg atccgcgccc
    23641 cgggcagtcg atccagactg gcgaacctga gtatgtcatt ccagatgtgc tggtgcgtaa
    23701 gcataacggt cactggacgg tagaactcaa cagtgacagc attccgcgtc tgcaaatcaa
    23761 ccagcactac gcctcgatgt gcaataacgc gcgcaatgat ggtgacagcc agtttatccg
    23821 cagcaatctg caagatgcca aatggttgat caagagtctg gaaagccgta acgatacgct
    23881 actgcgcgtg agtcgctgta tcgttgaaca gcagcaagcc ttctttgagc aaggcgaaga
    23941 atatatgaaa ccgatggtac tggccgatat cgcccaggct gtcgaaatgc atgaatcgac
    24001 gatatctcgc gtgaccacgc aaaaatacct gcatagtcca cgaggcattt ttgaactgaa
    24061 gtatttcttt tccagtcacg tcaataccga gggcggcggc gaagcttcct ccacggcgat
    24121 tcgtgcgctg gtgaagaaat taatcgcggc ggaaaaccca gcgaaaccgt tgagcgacag
    24181 caagttaacc tctttgctgt cggaacaagg tatcatggtg gcacgccgca ctgttgcgaa
    24241 gtaccgagag tctttatcca ttccgccgtc aaaccagcgt aaacagctcg tttgacccaa
    24301 ccgataagga agacactatg cagctcaaca ttaccggaaa taacgtcgag atcaccgagg
    24361 cactgcgcga atttgttaca gccaaatttg ccaaacttga gcaatatttt gaccgaatca
    24421 accaggtcta tgttgttctg aaagtggaga aagtcaccca cacctcagat gcaacactgc
    24481 atgtaaacgg tggtgaaatt catgccagcg cggaaggtca ggacatgtac gctgccattg
    24541 atggcttaat tgataagctg gcacgccagt tgaccaaaca taaagataaa ctgaaacaac
    24601 actaattgtc cgggcaatta gcatgtgcat ggcggcctgt tgtgcggcac aacgggccat
    24661 ttgtacggtt aatgctccga gcctgttcca ctgtttgagt gggcaggttc ttaggtgaaa
    24721 ttatgacaaa taatgataca actctacagc ttagcagtgt tcttaacagg gaatgtacgc
    24781 gaagccgcgt ccactgtcag agcaaaaaac gcgccctgga aatcatcagc gagctggcgg
    24841 cgaaacaact tagcctgccg cctcaggtgg tttttgaagc tatcctgacg cgggaaaaaa
    24901 tgggcagtac cggtatcggc aatggtattg ccattccgca tggcaaactg gaagaagata
    24961 ctctgcgtgc cgttggcgtt ttcgttcagc tcgaaacgcc tatagctttc gatgccatcg
    25021 acaaccaacc ggtggactta ctttttgccc tgctggtgcc ggcagaccaa actaaaacgc
    25081 acctgcatac tctgtcgctg gtggcgaaac gtctggcgga caaaaccatc tgccgccgtt
    25141 tacgcgcagc ccagagcgat gaagagctgt atcaaatcat tacggatacc gaaggtactc
    25201 cggatgaagc gtagttattc ggtaatgtct cttttagacg ttgtgaggag aaacagtaca
    25261 tggtactgat gatcgtcagc ggacgttcag gttcaggtaa atctgtcgcc ctgcgtgcgc
    25321 tggaagatat gggtttttac tgcgtggata accttcccgt agtgttgtta cccgatctgg
    25381 ctcgaaccct ggccgatcgc gagatttctg ccgccgtcag cattgatgtt cgtaatatgc
    25441 cggagtcacc agaaatattc gaacaggcga tgagtaacct gcctgacgct ttctcaccgc
    25501 aactcctgtt cctggatgcc gaccgtaata ccttaattcg tcgttacagt gacacgcgcc
    25561 gactgcatcc gctttccagc aaaaacctgt cgctggaaag tgctatcgac aaagaaagcg
    25621 atctgctgga gcctctgcgt tcgcgagcgg atctgattgt cgatacctca gaaatgtccg
    25681 ttcacgagct ggctgaaatg ctgcgtaccc gtctgctggg taaacgtgaa cgcgaactga
    25741 ccatggtctt tgagtctttc ggcttcaaac acggtatccc tatcgatgca gattacgtct
    25801 ttgacgtgcg cttcttgccg aacccgcact gggatccgaa actgcgtcca atgacaggtc
    25861 ttgataaacc tgtcgccgcg ttcctcgacc gccacacaga agtacacaat tttatctacc
    25921 agacgcgaag ctatcttgag ctatggttac ctatgctgga aaccaacaac cgtagctact
    25981 tgacggtcgc cattggttgt accggcggga agcaccgttc ggtgtatatt gcagagcaac
    26041 tggcagacta cttccgctcg cgcggtaaaa acgtccagtc acgccatcgt acgctggaaa
    26101 aacgtaaacc atgaccgtca agcaaactgt tgaaatcaca aacaagctgg gcatgcatgc
    26161 ccggcctgca atgaagctgt ttgaattaat gcagggtttt gacgctgaag tgctgttacg
    26221 taatgacgaa ggcaccgagg ctgaagccaa cagcgttatt gcgctgctga tgttggattc
    26281 tgccaaagga cggcagattg aagttgaagc gaccggtcca caggaagagg aagcactggc
    26341 cgccgttatc gccctcttta actctggttt tgatgaagat taatcttcat cactttgaca
    26401 ttcaaatccc ttcaagctcc cccggcaata agaaaagcgc gtactgaaat cgttctcaat
    26461 caacgtcatt tgtacatttt gtgcgctttt cactttcaga agaaccttaa gaaaacctta
    26521 agaggcattg tttaggtttt gtttaagtta atcgaccata ctggagatcg tcagaaaata
    26581 tttccaggag atggcatgat tcgcttatct gaacaaagtc ccctgggcac cggaagacat
    26641 cggaaatgtt atgcgcatcc ggaagatgcc caacgctgta tcaagattgt ctaccatcgt
    26701 ggcgatggtg gcgataaaga gatccgtcgt gagttaaagt actacgcgca tcttggtcgc
    26761 cggttaaaag actggagtgg aataccgcgc tatcacggta ccgttgagac ggattgcgga
    26821 accggttatg tctacgatgt gattgccgat ttcgacggca agccttccat tacactgacc
    26881 gaatttgctg aacaatgccg ttacgaagaa gacgttgcac aactgcgcca gttactgaaa
    26941 cagctgaagc gttatttgca ggataaccgt atcgtgacga tgtcgctgaa gccgcagaat
    27001 atcctctgtc accgcatcag tgagtcagag gttatccccg tcgtctgcga caacattggt
    27061 gaaagcacat tgattccact ggcgacctgg tcgaagtggt gttgtctgcg taagcaagag
    27121 cggttatgga aacgatttat cgcccaaccg gcgctggcta ttgccctgca gaaagatctg
    27181 caacctcgtg agagcaaaac gctggccctc gcttcgcgcg aagcttaatc cagctggtgt
    27241 tgttgcataa acggctcacc gcctaactga tacatctgcc gtaaaatcca cgcctgacgg
    27301 ctacgcatgt agcccgatgg cgtggagact ttgaaacgaa gtggattagg taatacagct
    27361 gccagtaatg cagcttccga ccgagtaagt ttgctcgcgg gtttgtggaa atagcgttgt
    27421 gccgcagctt cgacgccaaa cacaccgtcg ccaaattcgg cgatattcag gtaaaccgtc
    27481 aggatacgct ttttgctcca gacagtttct atccccagcg ttaatccggc ttccagccct
    27541 tttcgaaccc agctacgccc atcccataaa aagagatttt tggctgtctg ttgagaaatc
    27601 gttgaagcac cgcgaatacg gttttcattg tgctcattgt gcgccagggc tttctcaatg
    27661 gaagcgacat caaagcccca gtgctcagga aatttctgat cttctgcggc aatcaccgcc
    27721 agtcccatcc acggcgagat ttgatccata ctgacccagt cagaatgtgc cacgtaacga
    27781 aaattgccat gcagccaggc gctgacctgt cgctcgacca ttaccgctga gaagggaaca
    27841 ggcgcaacgc taaacaacgc gatgcccccg ccccagaaaa cggcgaggac aaccattaac
    27901 cgcaaaagaa aacggcgaac gaaactaaac accgttaagc ggcttttact cattcagcca
    27961 gaaccagcac gcgggaaacc agcttatcaa tgccgctcgc cgcttctgca atgttctgcg
    28021 ccagcatata tgctggggtg gtgacaatct tattgtcttc atcaaccacg atatcatcga
    28081 caggacacgg cacatgctcc gcgcccatct cttccagcac ttctgcggta tcgatatcag
    28141 taccgatggt caaacgcagc gggaaatcga aaattttcgg cagcatcgcc ggggcaatac
    28201 acataaaacc aagcggtttt ccggcatgat gcatcgcttg tgccagcgcc tttaattcac
    28261 ggtcaacggt gcattcgcga ccaagactgg caaaattact taaattcttt gccgcgccaa
    28321 ccccccccgg cacaatcaac gcatccagtt cagcggcatc ggcctgggcc agaggacgga
    28381 tttcaccgcg cgttattcgt gccgcttcaa tcagcacatt gcgcgtttcc gtcatcgctt
    28441 cgccagttaa atgattgata acatcaacct gctgcttatc aggtgcaaag cagaccgcct
    28501 gcgcaccgct gcgtgaaata gctaacaacg tcaacaccgc ttcatgaatt tcagaaccgt
    28561 catagacgcc gcatccgctc agaattacgc caattttctt cattgtgatc atcctttcgc
    28621 aattgactga aacacattaa tttttttaat aaaaatggta cgcatcacac atttaactga
    28681 ttcacgtaac aaatcattta agttttgcta tcttaactgc gtgcggcctg aaaaacagtg
    28741 ctgtgccctt gtaactcatc ataataattt acggcgcagc caagatttcc ctggtgttgg
    28801 cgcagtattc gcgcaccccg gtctagccgg ggtcattttt tagtggcttt tgccacccac
    28861 gctttcagca cttctacgtc gtgacgccac tcttctttca tctcttcaat ccattcaccg
    28921 acgttatctt cccaggcagg aaggtccgga gactgaattt gctgacctag ctgttgcaga
    28981 tggcgtaacc ccactgaccc cgccgcacct ttaattttat gtccttcctc aacaatgcct
    29041 tttttatcct gcgccgtcag attcgactcc agcacgctga catagcccgg catcatcttc
    29101 tcaaacaccg ctaacccgtc ggtgatcagc ttcggtccta caagttcgag atactgttcc
    29161 agcatgggaa tatcgagcaa tgcttctgat ttactgttct cttctgtcgt caccgtactc
    29221 tcctcatcat cctgggtatc ccagaatttc ttgatcatcg cggttagcgc cggaacagaa
    29281 agcggcttac tcagcacatc atccattcca gcattgaggt actcttgctt gtctttcagc
    29341 acgttagcgg ttaaagccac cagcggcggt aaatcctcgc gcggataacg cttcgtcagt
    29401 tcacgagaga tatccagccc ggtcatatct ggcaactgaa tatccagcaa caccaggtcg
    29461 tattcgcccg gtttaaacat ctccagcgcc gccttgccgg tcatggcgac atcaacgctg
    29521 ttacctaatt tttccagcac agaacgcgcg acaatcacgt tcagttcaat gtcttccacc
    29581 agcagcacat tcagcgccgg taaaggcata tcgtcttcat caaacgcatc atcgacctct
    29641 tctgctaccg acggtgcgtg gatcgtcaac gtaaaggttg aacctttacc ctgttcgctg
    29701 gtaaccgtaa tatcgccgcc catatttttc gccagacgac gagaaacggc cagaccaata
    29761 ccggtgccgg tggcaggttt accgccatgg ctgtctttca cctggtaata catggcgaag
    29821 attttatcca gctcatcctg cggaatgccg ataccagaat cttccacttc aaaatgcagc
    29881 atatcgcctt catcgtagcg cacgcgcacg gtaacctggc cttgctgggt gaatttgacg
    29941 gcgttactga tgaggttcca caggatctgc cgtaaacgcg tcccgtcggt aatgacctga
    30001 tgtggtaatg gcagtgtagg ctccaggtta aagcgcaatc ctttttgttg cgcctgcaag
    30061 gcggagagat tttccagatc ggcaaggaag ctggtgaaat caaccggctg attatcaagc
    30121 tggaccttgc gccgttccat cttatccatg tcgataatat cgttgaagat attccccagc
    30181 gtgacggccg aaacatggat agttttgaga tatttttcct gctcggcggt gagttcggta
    30241 tccagtagga tgcggctcag accgacgata ccgttcagcg gtgtacgcaa ttcgtgactg
    30301 atggtggaaa taaacgtcgt tttgtcgcgg ctggcgcgtt caagcgcatc ctgataccgc
    30361 ttacgctcgg taatgtcgcg accaaagccc atcaaaccgt gacgtttacc cacgcggtcg
    30421 tagtacggca ctttacgaat ttcaaagcag gctttgcgtc cgtcgggata atccagccac
    30481 tgctcatagg tcagtgacac attatgacgg aacacttttt catcggtttc aatgactttt
    30541 gcggcggctt ccggtgagta aacatcagca ggtttcagat gaaccagttg tttttcgctt
    30601 tttccggtca gcagctccat cgcgcggtta cagccggaaa actctttatc ttcgttacga
    30661 taaaaaacca ggtcgggtga agcatcaagg aaggaacgta agaatgagga ttgctgctcg
    30721 agctgaattt gtgtctcttc gcgctctttg atttcaattt tcagttggcc gaaggtttcc
    30781 tgcagttctg cttccgcttt ttcacgaacg gcgatttcct gatttagctg ggcaatatta
    30841 tcttttaact gaacgttgag gctcaaatcg cgctcgcgca tctcctccag tttttgcacc
    30901 agccgtgaca gacgttgtcg tgactcctcc agttgctcga cgaccaccga tagaaagtag
    30961 accgcccacg gcgtaatcag caaaccaaag aagatagaac gaataacatc aatgctttcg
    31021 acctgaccat gcagcaccat ggttaccgcc atttgtacca caatggcaag aacgacgagg
    31081 gccagcgcca gcaacattga gaagcgcacc agacctaact tcatcatcag gtcaacataa
    31141 tactgcgcca gcagacgaat ttgcttcatt agggaattcc ttcacgacaa cctgccccaa
    31201 taatacccaa ttctgacagt tacgttataa attgtgtgag aaatgcggaa ttcccctcaa
    31261 cgacctactc cgttggaggt atccacggac gtcccagcgc cgatccctgt acgccatgtt
    31321 cattcagata gcgatccagc tcgaccattc ccgtccatcg attttcgcac cacagcggag
    31381 caagcagcgt cgggcgacgg gcgctggcgg aaatgcggtg gtagatcact tccggtggcg
    31441 tatggcgaat catttctccg gcagtgagcg tgtaatcctc cagttcaata ccgttcaaac
    31501 gccccgcttc ccaggctttc gccataatac tgcctttcac aatatgcagc ggatgcagct
    31561 ttatgccgtc cacgccggtt tcaaccactc gctccagcgt ttgcaaacat tcagcctgac
    31621 cttcgccagg caggccgaca attaagtggg aacacacctt cagcccgcgc tgacgtgcca
    31681 gctgggttgt acgctgataa caggcaaaat catggccgcg gttgatgcga tgcagggttt
    31741 tgtcgtgggc ggtttgtagc cccagctcca gccacacttc gtagccctgg tccttatatt
    31801 cgcaaagcag atccagcacc gcatccggca cgcagtccgg gcgagtacca acacacaaac
    31861 cgacaatatt ggcctggctc accgcctgct gatacataga acgcagcacc tgaacttccg
    31921 caaaggtgct ggtatacgcc tgaaagtagg ccagatagcg tttagcgcgg ttaactaaat
    31981 tcgcctggtg cgccagttgc tcggcaatgg aacgatgttg ctgcgcttca tcagcaaatg
    32041 aggcaacatt acagaatgtg cagccgccac gcccgatgct accgtcacgg ttagggcagc
    32101 taaaaccgcc gtgcagcgtc agcttatgca ccttttgccc ataacgacgg gtgagatcac
    32161 caccaaacat attgactaat ttctgtaact gcataatctg atagaccgcg ccttgaaaag
    32221 aggccaaagc ctgccatttt tagctcaatt cggcgatgac ctggatcaat cgtcccggcc
    32281 tgcttttacc aactgcataa tcaatcaaaa ttaccgaaat ttcatgcata atcacataaa
    32341 tcacttttgc ttatcttgtg tcagattttt ttatctcctt atggatttta ggcaaaaaca
    32401 gtagcatgaa acgtcattac caattaaggc agtataaaat gctggttttc tcgtcagttc
    32461 aaggcaggat aagggttaac acgccttaat gacagtcagg aattggctgt ttctctaacg
    32521 acgctccttt tagccttaat tagaaaatct attttaattt cagtcaatta ataaaga
//
LOCUS       DAPGOF010000040        32306 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.040, whole genome shotgun sequence.
ACCESSION   DAPGOF010000040 DAPGOF010000000
VERSION     DAPGOF010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.040"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(71..1831)
                     /locus_tag="RHV13_001607"
     CDS             complement(71..1831)
                     /locus_tag="RHV13_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HDV1241156.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRDTILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            1961..2653
                     /locus_tag="RHV13_001608"
     CDS             1961..2653
                     /locus_tag="RHV13_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HDV1241157.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMNAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            2852..3940
                     /gene="serC"
                     /locus_tag="RHV13_001609"
     CDS             2852..3940
                     /gene="serC"
                     /locus_tag="RHV13_001609"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HDV1241158.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTAADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVNRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            4011..5294
                     /gene="aroA"
                     /locus_tag="RHV13_001610"
     CDS             4011..5294
                     /gene="aroA"
                     /locus_tag="RHV13_001610"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDV1241159.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDAPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            5463..6227
                     /gene="ycaL"
                     /locus_tag="RHV13_001611"
     CDS             5463..6227
                     /gene="ycaL"
                     /locus_tag="RHV13_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HDV1241160.1"
                     /translation="MKNTKLLLAIAASAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            6400..7083
                     /gene="cmk"
                     /locus_tag="RHV13_001612"
     CDS             6400..7083
                     /gene="cmk"
                     /locus_tag="RHV13_001612"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDV1241161.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            7194..8867
                     /gene="rpsA"
                     /locus_tag="RHV13_001613"
     CDS             7194..8867
                     /gene="rpsA"
                     /locus_tag="RHV13_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDV1241162.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            9027..9311
                     /gene="ihfB"
                     /locus_tag="RHV13_001614"
     CDS             9027..9311
                     /gene="ihfB"
                     /locus_tag="RHV13_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HDV1241163.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            9518..11782
                     /locus_tag="RHV13_001615"
     CDS             9518..11782
                     /locus_tag="RHV13_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HDV1241164.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILLAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLASFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHIWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSAHWPLQGSNDKGNNHSCVVK
                     VDDGTNSILLTGDIEAPAEQKMLSRYWQQVQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYTWLDTPHQGQLTVDFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            11819..13567
                     /gene="msbA"
                     /locus_tag="RHV13_001616"
     CDS             11819..13567
                     /gene="msbA"
                     /locus_tag="RHV13_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HDV1241165.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     LERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            13564..14550
                     /gene="lpxK"
                     /locus_tag="RHV13_001617"
     CDS             13564..14550
                     /gene="lpxK"
                     /locus_tag="RHV13_001617"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HDV1241166.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     STGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLVQLTSLASGN"
     gene            14587..15819
                     /locus_tag="RHV13_001618"
     CDS             14587..15819
                     /locus_tag="RHV13_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HDV1241167.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRKTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDSVA"
     gene            15871..16053
                     /gene="ycaR"
                     /locus_tag="RHV13_001619"
     CDS             15871..16053
                     /gene="ycaR"
                     /locus_tag="RHV13_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HDV1241168.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            16050..16796
                     /gene="kdsB"
                     /locus_tag="RHV13_001620"
     CDS             16050..16796
                     /gene="kdsB"
                     /locus_tag="RHV13_001620"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HDV1241169.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKGLETVGDNFLRHLGIYGYRAGFIRRYVTWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAHEVPGTGVDTPEDLERVRAEMR"
     gene            16950..17843
                     /locus_tag="RHV13_001621"
     CDS             16950..17843
                     /locus_tag="RHV13_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HDV1241170.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNKTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(17820..18599)
                     /gene="elyC"
                     /locus_tag="RHV13_001622"
     CDS             complement(17820..18599)
                     /gene="elyC"
                     /locus_tag="RHV13_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HDV1241171.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGSSGEPRQE"
     gene            18735..19520
                     /gene="cmoM"
                     /locus_tag="RHV13_001623"
     CDS             18735..19520
                     /gene="cmoM"
                     /locus_tag="RHV13_001623"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HDV1241172.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            19517..20839
                     /gene="mukF"
                     /locus_tag="RHV13_001624"
     CDS             19517..20839
                     /gene="mukF"
                     /locus_tag="RHV13_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HDV1241173.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPPDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            20820..21524
                     /gene="mukE"
                     /locus_tag="RHV13_001625"
     CDS             20820..21524
                     /gene="mukE"
                     /locus_tag="RHV13_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HDV1241174.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            21524..25984
                     /gene="mukB"
                     /locus_tag="RHV13_001626"
     CDS             21524..25984
                     /gene="mukB"
                     /locus_tag="RHV13_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HDV1241175.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQSAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEVRAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     EQEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNNDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            26245..28092
                     /gene="ldtD"
                     /locus_tag="RHV13_001627"
     CDS             26245..28092
                     /gene="ldtD"
                     /locus_tag="RHV13_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDV1241176.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQLLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALSTPPLSVINQWQQALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPTPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            28273..28821
                     /locus_tag="RHV13_001628"
     CDS             28273..28821
                     /locus_tag="RHV13_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HDV1241177.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            28848..29495
                     /gene="gloC"
                     /locus_tag="RHV13_001629"
     CDS             28848..29495
                     /gene="gloC"
                     /locus_tag="RHV13_001629"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HDV1241178.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(29717..30907)
                     /gene="aspC"
                     /locus_tag="RHV13_001630"
     CDS             complement(29717..30907)
                     /gene="aspC"
                     /locus_tag="RHV13_001630"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HDV1241179.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRALTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(31092..32165)
                     /gene="ompF"
                     /locus_tag="RHV13_001631"
     CDS             complement(31092..32165)
                     /gene="ompF"
                     /locus_tag="RHV13_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HDV1241180.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKDNGVNSYGGNGDKTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQKGNK
                     TRLAFAGLKFADAGSIDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERAGIPERSNGDGVGGSISYEFEGFGIVGAYGAAD
                     RTDAQEAEFRGQGKKAEQWATGLKYDANNIYLAANYGETRNATPIEGGFANKTQDVLL
                     VAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVDYIINQ
                     IDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         7833 a         7878 c         8708 g         7887 t
ORIGIN      
        1 gcattaaaaa aacgccccgt aatataactc agacgaatca ttaagcctac attgcgtagg
       61 ctatttgatt ttattttgcc cagaatgctg ctttggcgcg ctgcagcttt tcgtaggcct
      121 tcaacagcga ctgatgtgca gcaaacgcgt gcagatcgct atctactggt tgcaagccat
      181 aaaacgccgc ttcgccgctc attgccgcac tggcggcttc cacggcatct gcgccgtaca
      241 tgcgaacaaa cgcattcaga tattgcagtg gctggcgatc ttcttcctgc gccagtaata
      301 acaacgtttg caggcagcga taatagttgg cgcgttccgg gctgaatacc gatgagttaa
      361 actccatcgt ccattcggtc cagaccagag cctgttccag atcgccacca gccagcgcca
      421 gcatggcttt taattcaccg atacgcaggg tgtaccaacc gttatccgat ccggtcgcca
      481 gacccaacag ctcacgcacg cgggtaaagt catcaaaacc ttcttcatcc agttgctcga
      541 tgaggttcag gtaatcttct ttttcccact cgctgcctgg tagcgagaga atggtgtcgc
      601 gcagatgact acccatactg ttattcgcta accacaaatc ttcagccgga taaatatcgg
      661 acatgccagg cacgataata cggcaagcat aaacgcccag atgctcgtaa tcggcaatat
      721 aaacttcttt atcttctttg ttgaagatag ccatcagcgt agcaaactct tcttctgtgg
      781 tgccggagaa attccagtcc acaaacggat aatcggcatc ctgcttgaac aggtcccagg
      841 agattaaacc gctggaatcg ataaagtgcg tttcgaggtt ggtatgttca gcaacttctt
      901 catcatcgaa ggttggcgga gtaaacacat ccaaatcttt caggccacga ccttgcagca
      961 gctcggtcac ggtacgttcc agtgctacgc caaaatcagg atgcgcaccg aaagaggcaa
     1021 agcatgtacc gttagcagga ttaaacagta ccacgcaaat caccggatac tggccgccaa
     1081 gcgaaccgtc ataagcgaag atcgggaaac cttctgcttc cagtgtttcg atcgcttcaa
     1141 ccaccgctgg gtaacgcgcc agcacgtctg ccgggatttc cggcaagctg atgctttcag
     1201 caataatgcg gtttttcacg tagcgttcga aaacttcgga caacccctga acgcgtgctt
     1261 cgttgcgggt attgccagcg gacataccgt tagaaacgta caggttacca atgatattca
     1321 tcggaatata aacggtctga ttgtcggact ggcgcgtaaa cggcagaccg caaataccac
     1381 gatcttcgtt accggattgt aggtcaatca gcatactgcc ggtcaattca ttctccggat
     1441 cgtaaaatgc gcgcagacgg tcatcgagca gcccttctgg cacatcgtca ttttcggtca
     1501 gtgggaacca tttttcgttg ggataatgca caaatggacc gttggcgatg gtttcgccca
     1561 gccagaaatc agcaaaaaag tagttggttg agagacgctc gaaatattca ccgagtgcag
     1621 aagccagcgc cgctttcttg gttgcgcctt taccgttggt aaaacacagt gcgcactctt
     1681 tgtcgcgaat atgtacagac cagacgttag gcacgggatt cagccaggag gcctcttcaa
     1741 tctgaaagcc gaggtctgaa agtttttgct ggaagcgagc gatggaatct tccagtgcgg
     1801 catctttgcc ggggataaat gtttgcgtca tgaaaatcac tttagtcgta cggaaagcgc
     1861 gcaataatac gggttttatc tcaaaggcgc tatcaccgcc gccatgccga tgaactgttg
     1921 actatgcttt tagcagataa accacgataa gagcataaaa atgaaggcgt tcgatctcca
     1981 ccgtatggca tttgataaag tgccttttga tttccttggc gaagttgcac tacgtagtct
     2041 gtataccttt gtactggttt ttttgttcct caaaatgacc ggaagacgcg gagtgcggca
     2101 gatgtcgctg tttgaagtgt taatcattct gacgctgggg tcagcggcgg gagatgtggc
     2161 gttttatgat gatgtgccga tggtcccggt acttatcgtc tttatcactc tggcgttgtt
     2221 ataccgcctg gtaatgtggt tgatggcaca cagtgaaaaa ctggaagatc ttctggaagg
     2281 taagccggtt gtcattattg aagatggcga gctggcctgg tcgaaactca ataactccaa
     2341 catgacggaa tttgagttct ttatggagct acgattgcgc ggtgtagagc agttggggca
     2401 agtacgtctg gcgattctcg aaaccaacgg gcaaatcagt gtctatttct ttgaagatga
     2461 caaggtgaaa ccgggtttac ttattttacc cagtgattgt acgcagcgtt acaaagtggt
     2521 gccggagtcg gcggactatg cctgcatccg ttgtagtgaa atcattcata tgaacgcggg
     2581 ggaaaaacaa ttatgtccgc gctgtgcaaa tccagaatgg acgaaggcaa gtcgggctaa
     2641 acgggtgacc tgacagcaaa aatatcggtt ttttggtaat aatccgagag attctgttgt
     2701 gtgacgcgtg ccgcattttt gccctcaacg gttttactca ttgcgatgtg tgtcactgaa
     2761 tgataaaacc gatagccaca ggaataatgt attacctgtg gtcgcaatcg attgaccgcg
     2821 ggttaatagc aacgcaacgt ggtgagggga aatggctcaa atcttcaatt ttagttctgg
     2881 tccggcaatg ctaccggcag aggtgcttaa acaggctcaa caggaactgc gcgactggaa
     2941 cggtcttggt acgtcggtga tggaagtgag tcaccgtggc aaagagttca ttcaggttgc
     3001 agaggaagcc gagaaggatt ttcgcgatct tcttaatgtc ccctccaact acaaggtatt
     3061 attctgccat ggcggtggcc gcggtcagtt tgctgcggta ccgctgaata ttctcggtga
     3121 taaaaccgcc gcagattatg ttgatgccgg ttactgggcg gcaagtgcca ttaaagaagc
     3181 gaaaaaatac tgcacgccta atgtctttga cgccaaagtg actgttgatg gtctgcgcgc
     3241 ggttaagcca atgcgtgaat ggcaactctc tgataatgct gcttatatgc attactgccc
     3301 gaatgaaacc atcgacggta tcgccatcga cgaaacgcca gacttcggca aagatgtggt
     3361 ggttgccgcc gacttctctt caaccattct ttctcgtcca atcgatgtta accgttatgg
     3421 tgtgatttac gctggcgcgc agaaaaatat cggcccggca ggcctgacaa tcgtcatcgt
     3481 tcgtgaagat ttgctgggca aagcgaatat cgcgtgtccg tcgattctgg attattccat
     3541 cctcaacgat aacggctcca tgtttaacac gccgccgaca tttgcctggt atctatctgg
     3601 tctggtcttt aaatggctga aagcgaacgg cggtgtagct gaaatggata aaatcaatca
     3661 gcaaaaagca gaactgctat atggggtgat tgataacagc gatttctacc gcaatgacgt
     3721 ggcgaaagct aaccgttcgc ggatgaacgt gccgttccag ttggcggaca gtgcgcttga
     3781 caaattgttc cttgaagagt cttttgctgc aggccttcat gcactgaaag gtcaccgtgt
     3841 ggtcggcgga atgcgcgcgt ctatttataa tgccatgccg ctggaaggcg ttaaagcgct
     3901 gacagacttc atggttgagt tcgaacgccg tcacggttaa tgccgaaatt ttgcttaatc
     3961 cccacagcca gcctgtgggg tttttatttc tgttgtagag agttgagttc atggaatccc
     4021 tgacgttaca acccatcgct cgtgtcgatg gcactattaa tctgcccggt tccaagagcg
     4081 tttctaaccg cgctttattg ctggcggcat tagcacacgg caaaacagta ttaaccaatc
     4141 tgctggatag cgatgacgtg cgccatatgc tgaatgcatt aacagcgtta ggggtaagct
     4201 atacgctttc agccgatcgt acacgttgcg aaattatcgg taacggcggt ccattacacg
     4261 cagaaggtgc cctggagttg ttcctcggta acgccggaac ggcaatgcgt ccgctggcgg
     4321 cagctctttg tctgggtagc aatgatattg tgctgaccgg tgagccgcgt atgaaagagc
     4381 gcccgattgg tcatctggtg gatgcgctgc gcctgggcgg ggcgaagatc acttacctgg
     4441 aacaagaaaa ttatccgcca ttgcgtttac agggcggctt tactggcggc aacgttgacg
     4501 ttgatggctc cgtttccagc caattcctca ccgcactgtt aatgactgcg cctcttgcgc
     4561 cggaagatac ggtgattcgt attaaaggcg atctggtttc taaaccttat atcgacatca
     4621 cactcaatct gatgaagacg tttggtgttg aaattgaaaa tcagcactat caacaatttg
     4681 tcgtaaaagg cgggcagtct tatcagtctc cgggtactta tttggtcgaa ggcgatgcat
     4741 cttcggcttc ttactttctg gcagcagcag caatcaaagg cggcactgta aaagtgaccg
     4801 gtattggacg taacagtatg cagggtgata ttcgctttgc tgatgtgctg gaaaaaatgg
     4861 gcgcgaccat ttgctggggc gatgattata tttcctgtac gcgtggtgaa ctgaacgcta
     4921 ttgatatgga tatgaaccat attcccgatg cggcgatgac cattgccacc gcggcgttat
     4981 ttgcaaaagg aaccaccacg ctgcgcaata tctataactg gcgtgttaaa gagaccgatc
     5041 gcctgtttgc gatggcaaca gaactgcgta aagtcggtgc ggaagtagaa gaggggcacg
     5101 attacattcg tatcactcca ccggaaaaac tgaactttgc cgagatcgcg acatacaatg
     5161 atcaccggat ggcgatgtgt ttttcgctgg tggcgttgtc agatgcacca gtgacgattc
     5221 ttgatcccaa atgtacggcc aaaacatttc cggattattt cgagcagctg gcgcggatta
     5281 gccaggcagc ctgaatgagc aacgggcaat aaatagccaa atctttcttt atcaaaacgc
     5341 cggcacattg ccggcgtttt ttttcggacc ttgtgagtca ttttgattaa tggtagcgtc
     5401 gcttgtcaat gtaagttgtt gatacataat atttatatat gattaatcaa cggatgattc
     5461 acatgaagaa tactaaatta ctgctggcga ttgcggcctc tgcagcatta ctgacagggt
     5521 gtcaaaatac ccacggtatt gataccaata tggctatcag ctccggttta aatgcctata
     5581 aagcagcaac attaagcgat gccgatgcaa aagcgattgc caatcagggc tgtgccgaaa
     5641 tggacagcgg caatcaagtc gcaagtaaat ccagcaagta cggtaaacgt ctggcaaaaa
     5701 tcgccaaagc attgggtaac aatattaatg gcacgccggt caactataag gtttatatga
     5761 ccagcgacgt caacgcatgg gcgatggcga acggctgtgt tcgtgtttac agtggcctga
     5821 tggacatgat gaacgataac gaaattgaag gcgttctggg ccatgaactg ggccacgtcg
     5881 cgttgggtca ctcgctggct gaaatgaaag cttcttatgc gatcgttgcc gcacgcgatg
     5941 ccatttcagc taccagcggt gtggcttccc agctttcccg ctcacaattg ggtgatatcg
     6001 cagaaggcgc tatcaatgcg aaatactccc gcgataaaga gtccgaagca gatgatttct
     6061 cctttgatct gttgaagaaa cgtggcatca gcacccaggg gctggttggc agctttgaaa
     6121 aactggccag cctggatggc ggtcgcaccc agtccatgtt tgactctcac ccaccatcaa
     6181 cagagcgtgc gcaacacatc cgtgatcgta tcgcctctgg taagtaaatc attgtcatct
     6241 ttcgggctgg tcttctgcca gcccgctata attgcgcaat aaatccccat ctgaatacag
     6301 acaaaactgg tttttgcaca caacgttaac gatttgtggc gtcggcgcgt ataatgcgcg
     6361 cggttatgtt aacggtacgc ctgttttaag gagataaaga tgacggcaat tgccccggtt
     6421 attaccattg atggcccaag cggtgcaggg aaaggcacct tgtgtaaggc tatggcggaa
     6481 gcgttgcaat ggcatctgct ggattcgggt gcaatttatc gcgtactggc actagcggca
     6541 ttacatcacc atgttgatgt tgcatcggaa gatgcgctgg tgccgctggc atcccatctg
     6601 gatgtacgtt ttgtgtcaac caatggcaat ctggaagtga tcctcgaagg ggaagatgtc
     6661 agcggcgaaa ttcgtactca ggaagtggcg aatgctgctt cacaagtcgc ggcattccca
     6721 cgcgttcgtg aagcattatt gcgtcgccaa cgcgcgtttc gcgaattacc aggcctgatt
     6781 gccgatggcc gcgacatggg aacggtggta ttccctgatg cgccagtgaa aattttcctt
     6841 gacgcctcct cggaagaacg tgcgcatcgc cgcatgctac agttgcagga gaagggcttt
     6901 agtgttaact ttgagcgcct tttggccgag atcaaagaac gcgacgaccg cgatcgtaac
     6961 cgagcggtag cgccactggt tccggcagcc gatgctttag tgttggattc caccacctta
     7021 agcattgagc aagtgattga aaaagcgcta caatacgcgc gccagaaatt ggctctcgca
     7081 taagcgaccg aatttgcagt acccccgttg caatggaatg acagcgggta tgttaaacaa
     7141 ccccatccgg catggagcca ggtggacgtt aaatataaac ctgaagatta aacatgactg
     7201 aatcttttgc tcaactcttt gaagagtcct taaaagaaat cgaaacccgc ccgggttcta
     7261 tcgttcgtgg cgttgttgtt gctatcgaca aagacgtagt actggttgac gctggtctga
     7321 aatctgagtc cgccatcccg gctgagcagt tcaaaaacgc ccagggcgag ctggaaatcc
     7381 aggtaggtga cgaagttgac gttgctctgg acgcagtaga agacggcttc ggtgaaactc
     7441 tgctgtcccg tgagaaagct aaacgtcacg aagcctggat cacgctggaa aaagcttacg
     7501 aagatgctga aactgttacc ggtgttatca acggcaaagt taagggcggc ttcactgttg
     7561 agctgaacgg tattcgtgcg ttcctgccag gttctctggt agacgttcgt ccggtgcgtg
     7621 acactctgca cctggaaggc aaagagcttg aatttaaagt aatcaagctg gatcagaagc
     7681 gcaacaacgt tgttgtttct cgtcgtgccg ttatcgaatc cgaaaacagc gcagagcgcg
     7741 atcagctgct ggaaaacctg caggaaggca tggaagttaa aggtatcgtt aagaacctca
     7801 ctgactacgg tgcattcgtt gatctgggcg gcgttgacgg cctgctgcat atcactgaca
     7861 tggcctggaa acgcgttaag catccgagcg aaatcgtcaa cgtgggcgac gaaatcactg
     7921 ttaaagtgct gaagtttgac cgcgaacgta cccgtgtatc cctgggcctg aaacagctgg
     7981 gcgaagatcc gtgggtagct atcgctaaac gttatccgga aggtaccaaa ctgactggtc
     8041 gcgtgaccaa cctgaccgac tacggctgct tcgttgaaat cgaagaaggc gttgaaggcc
     8101 tggtacacgt ttccgaaatg gattggacca acaaaaacat ccacccgtcc aaagttgtta
     8161 acgttggcga tgtagtggaa gttatggttc tggatatcga cgaagaacgt cgtcgtatct
     8221 ccctgggtct gaaacagtgc aaagctaacc cgtggcagca gttcgcggaa acccacaaca
     8281 agggcgaccg tgttgaaggt aaaatcaagt ctatcactga cttcggtatc ttcatcggcc
     8341 tggacggcgg catcgacggc ctggttcacc tgtctgacat ctcctggaac gttgcaggcg
     8401 aagaagcagt tcgtgaatac aaaaaaggcg acgaaatcgc tgcagttgtt ctgcaggttg
     8461 acgcagaacg tgaacgtatc tccctgggcg ttaaacagct cgcagaagat ccgttcaaca
     8521 actgggttgc tctgaacaag aaaggcgcta tcgtaaccgg taaagtaact gcagttgacg
     8581 ctaaaggcgc aaccgtagaa ctggctgacg gcgttgaagg ttacctgcgt gcttctgaag
     8641 catcccgtga ccgcgttgaa gacgctaccc tggttctgag cgttggcgac gaagttgaag
     8701 ctaaattcac cggcgttgat cgtaaaaacc gcgcaatcag cctgtctgtt cgtgcgaaag
     8761 acgaagctga cgagaaagat gcaatcgcaa ctgttaacaa acaggaagat gcaaacttct
     8821 ccaacaacgc aatggctgaa gctttcaaag cagctaaagg cgagtaattc tctgactctt
     8881 cgggattttt attccgaagt ttgttgagtt tacttgacag attgcaggtt tcgtcctgta
     8941 atcaagcact aagggcggct acggccgccc ttaatcaatg cagcaacagc agccgcttaa
     9001 tttgccttta aggaaccgga ggaatcatga ccaagtcaga attgatagaa agacttgcca
     9061 cccagcaatc gcacattccc gccaagacgg ttgaagatgc agtaaaagag atgctggagc
     9121 atatggcctc gactcttgcg cagggcgagc gtattgaaat ccgcggtttc ggcagtttct
     9181 ctttgcacta ccgcgcacca cgtaccggac gtaatccgaa gactggcgat aaagtagaac
     9241 tggaaggaaa atacgttcct cactttaaac caggtaaaga actgcgcgat cgcgccaata
     9301 tttacggtta agtttttact caaacttgaa cgcgagaaaa gcacctgtcg ggtgcttttt
     9361 tcatttctct aatcaggaac tggaagctgc ctcgcagagt tttgagcagt tttcaccctt
     9421 tcgttaaatt cttctgaatg tgcctcgggg aacgcaaaat tcccaccaga caaccgctca
     9481 acaaagttgc acactttcca taaacaggga ggggtgcatg aaaataacga cagtcggcgt
     9541 atgcataatt tgcggaattt ttccgttgct gattttgccc caattgcctg ggacattaac
     9601 ccttgcgttt ctgactctct tcgcctgcgt actggcattt atccctgtta aaaccgtccg
     9661 ttatatcgcg ctgacgttgc tgtttttcgt ttggggcata ttatcagcaa agcaaatttt
     9721 gttggcagga gaaaccttaa ctggcgcgac gcaggatgca attgttgaga tcactgctac
     9781 tgacggcatg accactcatt acggtcaaat tacttatcta caaggtcgac gtatattccc
     9841 tgcgccaggc cttgtgctgt atggcgaata tcttccgcaa gcggtttgtg ccggacaact
     9901 gtggtcaatg aaactcaaag ttcgtgccgt tcatggtcaa cttaatgatg gcggctttga
     9961 tagccagcgt tatgccattg cccagcatca gccgctcacc ggccgctttc tgcaggcaag
    10021 tgttattgaa ccgaattgta gcctgcgtgc acagtatctg gcgtcactac aaacaacgct
    10081 gcaaccctat ccgtggaatg cggttattct tggtttaggt atgggggaac ggttatccgt
    10141 ccctaaagaa atcaaaaata tcatgcgtga tactggaacg gcgcatttaa tggcgatatc
    10201 gggattgcac atcgcttttg cggcgttgct ggctgccgga ctcattcgca gtggacaaat
    10261 ttttctgcct gggcgctgga tccactggca aatgccatta attggcggaa tctgctgtgc
    10321 tgctttttat gcctggctga cgggaatgca acctcctgca ttgcgtaccg ttgtggcgct
    10381 tgctacgtgg ggaatgctta agttaagtgg gcgacagtgg agtggctggg atgtatggat
    10441 atgttgtctg gcggcaattt tgctgatgga tcctgttgcc attctctcgc aaagtttatg
    10501 gctctctgcc gctgcggtcg cggcattgat attttggtat cagtggtttc cctgtcctga
    10561 gtggcaactg ccgccggtat tgcgtgcagt tgtttccctc atccatctgc aactgggaat
    10621 cacacttctg cttatgcccg tgcaaatcgt catatttcat ggcattagtc tggcctcgtt
    10681 tattgcaaat ctattagcaa ttcccttggt gacatttatc acggttccgt tgatcctcgc
    10741 cgcgatggtt gtgcatttaa gcgggccgtt aatcctggag caagggttat ggtttcttgc
    10801 cgaccggtct ttggctttac ttttctgggg attaaagagt ttgccagaag ggtggatcaa
    10861 cattgctgaa cgttggcaat ggctatcatt ttccccatgg ttcttactgg tggtatggcg
    10921 attaaacgcc tggcgaacgt tgccagcaat gtgtgtggct gtaggcttgc tgatgtgctg
    10981 gccgctgtgg caaaaacctc gacctgacga gtggcaagtg tacatgcttg atgtcgggca
    11041 agggctggca atggtgatag ccagaaacgg caaagcgatt ctctatgaca cgggactggc
    11101 ctggcccgaa ggggatagtg ggcaacaact gattatcccc tggctccact ggcataatct
    11161 tgaaccggaa ggcgttattc tgagccatga acatctggat caccggggag ggctggattc
    11221 aatattgcat atatggccga tgttatggat cagaagtccg ttaaactggg aacatcatca
    11281 gccctgtgtg cgtggcgaag cgtggcaatg gcaaggattg cgtttcagcg cgcactggcc
    11341 tttacaaggt agcaacgata aaggaaataa ccattcctgt gtggttaagg ttgatgacgg
    11401 gacgaatagc attcttctaa ccggtgatat tgaagcccca gctgaacaaa agatgctaag
    11461 ccgttactgg cagcaagtgc aggcaacatt gcttcaggta cctcaccatg gcagtaatac
    11521 ctcatcatcg ttgccattaa ttcagcgagt gaatggaaaa gtggcactcg catcggcatc
    11581 gcgctataac gcatggcgac tgccctctaa caaagttaag catcgctatc aacaacaggg
    11641 ttatacgtgg cttgatactc ctcatcaggg gcaactaacg gtcgattttt cagcgcaagg
    11701 ctggcggatt agcagcctca gggagcaaat tttacctcgt tggtatcatc agtggtttgg
    11761 cgtgccagtg gataacgggt agaatatgcg gctatttcaa caaatgctgg ttttttgaat
    11821 gcataacgac aaagatctct ctacgtggca gacattccgc cgactgtggc caaccattgc
    11881 gccttttaaa gcgggtctga tcgtggcggg cgtagcgtta atcctcaacg cagccagcga
    11941 taccttcatg ttatcgctcc ttaagccact tcttgatgat ggctttggta aaacagatcg
    12001 ctccgtgctg gtgtggatgc cgctggtggt gatcgggctg atgattttgc gtggtatcac
    12061 cagctatgtc tccagctact gtatctcctg ggtatcagga aaggtggtaa tgaccatgcg
    12121 tcgccgcctg tttggtcaca tgatgggaat gccggtttca ttctttgaca aacagtcaac
    12181 gggtacgctg ttgtcacgta tcacctacga ttccgaacag gttgcttctt cctcttccgg
    12241 cgcactgatt actgttgtgc gtgaaggtgc gtcgatcatc ggcctgttca tcatgatgtt
    12301 ctattacagt tggcaattgt cgatcatttt gattgtgctg gcaccgattg tttcgattgc
    12361 gattcgcgta gtatcgaagc gttttcgcaa catcagtaaa aacatgcaga acaccatggg
    12421 gcaggtgacc accagcgctg aacaaatgct gaaaggccat aaagaagtat tgattttcgg
    12481 tggtcaggaa gtggaaacga aacgcttcga taaagtcagc aaccgaatgc gtcttcaggg
    12541 gatgaaaatg gtttcagcct cttccatctc tgatccgatc attcagctga tcgcctcttt
    12601 ggcgctggcg tttgttctgt atgcggcgag cttcccaagt gtcatggata gcctgactgc
    12661 cggtacgatt accgttgttt tctcttcaat gattgcactg atgcgtccgc tgaaatcgct
    12721 gaccaacgtt aacgcccagt tccagcgcgg tatggcggct tgtcagacgc tgtttaccat
    12781 tctggacagt gagcaggaga aagacgaagg taagcgcgtg ctcgagcgtg cgactggcga
    12841 cgtggaattc cgcaatgtca cctttactta tccgggacgt gacgtacccg ccttgcgtaa
    12901 catcaacctg aaaattccgg cagggaagac ggttgctctg gttggacgct ctggttcagg
    12961 taaatcaacc atcgccagcc tgatcacgcg tttttacgat attgatgaag gcgaaatcct
    13021 gatggatggt cacgatctgc gcgagtatac cctggcgtcg ttacgtaacc aggttgctct
    13081 ggtgtcgcag aatgtccatc tgtttaacga tacggttgct aacaacattg cttacgcacg
    13141 gactgaacag tacagccgtg agcaaattga agaagcggcg cgtatggcct acgccatgga
    13201 cttcatcaat aagatggata acggtctcga tacagtgatt ggtgaaaacg gcgtgctgct
    13261 ctctggcggt cagcgtcagc gtattgctat cgctcgagcc ttgctgcgtg atagcccgat
    13321 tctgattctg gatgaagcta cctcggctct ggataccgaa tccgaacgtg cgattcaggc
    13381 ggcactggat gagttgcaga aaaaccgtac ctctctggtg attgcccacc gcttgtctac
    13441 cattgaaaag gcagacgaaa tcgtggtcgt cgaggatggt gtcattgtgg aacgcggtac
    13501 gcataacgat ttgcttgagc accgcggcgt ttacgcgcaa cttcacaaaa tgcagtttgg
    13561 ccaatgatcg aaaaaatctg gtctggtgaa tcccctttgt ggcggctatt gctgccactc
    13621 tcctggttgt atggcctggt gagtggcgcg atccgtcttt gctataaact aaaactgaag
    13681 cgcgcctggc gtgcccccgt accggtagtc gtggttggta atctcaccgc aggcggcaac
    13741 ggaaaaaccc cggtcgttgt ctggctggtg gaacagttgc aacagcgcgg tattcgcgtg
    13801 ggggtcgtat cgcggggata tggtggaaag gctgaatctt atccgctgtt attgtcggca
    13861 gataccacca cagcacaggc tggtgatgaa cctgtgttga tttatcaacg cactgatgcg
    13921 cctgttgcgg tttctcccgt gcgttctgat gcggtaaaag ccattctggc gcaacaccct
    13981 gatgtgcaga tcatcgtaac cgacgatggt ttacagcatt atcgtctggc gcgtgatgtg
    14041 gaaattgtcg ttattgatgg tgtgcgtcgc tttggcaatg gctggtggtt gccggcgggg
    14101 ccaatgcgtg agcgagcggg gcgcttaaag tcagttgatg cggtaatcgt caacggcggt
    14161 gtcccccgca gcggtgaaat ccccatgcat ctgctgccgg gtcaggcggt gaatttacgt
    14221 accggtacgc gttgtgacgt tgctcagctt gaacatgtgg tggcgatggc agggattggg
    14281 catccgccgc gcttttttgc cacgctgaag atgtgcggcg tacaaccgga aaaatgtgta
    14341 ccgctggccg atcatcagtc tttgaaccat gcggatgtca gcgcgttggt aagcaccggg
    14401 caaacgctgg taatgacaga aaaagatgcg gtgaaatgcc gggcctttgc agaagaaaat
    14461 tggtggtatt tgcctgtaga cgcacagctt tcaggtgatg aaccagcaaa actgcttgtg
    14521 caactaacct cgctggcttc tggcaactag ttacgccgcg gcagcgttcg attgatggag
    14581 tcatgaatgt cgctgccgca cctctccctt gctgatgcgc gtaatcttca ccttgccgcg
    14641 caaggtctgt taaacaaacc ccgccgtcga gcgtcgttgg aggatattcc ggcaacgatc
    14701 tcccgcatgt ccttgctgca aatcgatacc atcaatattg ttgcccgtag cccatatctg
    14761 gtgcttttca gtcgtctggg aaattatcct gcccagtggc tggatgagtc tctggcgcgt
    14821 ggcgaattaa tggaatactg ggcgcatgaa gcctgcttta tgccacgcag cgactttcgt
    14881 cttattcgcc accgcatgct ggcacctgaa aaaatgggct ggaaatacaa agacgcctgg
    14941 atgcaggaac atgaggcgga aattgcacag ttaattcagc atattcatga taaggggccg
    15001 gtacgttcag ccgattttga gcatcctcgt aaaggtgcaa gcggctggtg ggaatggaag
    15061 ccgcataaac ggcatctgga aggtttattt actgccggaa aggtgatggt gattgaacgg
    15121 cgcaacttcc agcgcgttta tgatttaacc caccgtgtca tgcctgactg ggatgatgag
    15181 cgcgatctcg tttcgcaaac agaagcagaa atcatcatgc tggataacag tgcgcgtagc
    15241 ctgggaatat tccgcgaaca gtggctggca gattactatc ggctgaaacg tccggcactg
    15301 gcggcgtggc gcgaagcgag ggctgaacag cagcaaatca ttgctgtgca tgttgaaaaa
    15361 ttgggcaatc tttggctgca tgctgatttg ctgccgctac tcgagcgtgc gctggccgga
    15421 aagctcactg caacgcacag cgcggtactt tcgccttttg atcctgttgt ctgggatcgc
    15481 aaacgcgcag agcagctttt tgattttagc taccggctgg agtgctatac cccagcgccg
    15541 aaacgtcagt atggctattt tgttctgcca ttattacatc gtgggcaatt agttgggcga
    15601 atggatgcca aaatgcatcg caagacaggc atccttgaag ttatctctct gtggttacag
    15661 gaaggcatta aaccaacgac aatgctgcaa aaagggttac gtcaggcgat tactgatttc
    15721 gcgagctggc agcaggcaac gcgggtgaca ttaggacgct gcccgcaagg tctctttact
    15781 gattgccgca ctggctggga aatagactcc gtcgcataaa tgattatgat aagatttatg
    15841 cattcataac ccgatccatc tggaggaact atggatcatc gtctgcttga aatcattgcc
    15901 tgcccggttt gcaacggaaa actttggtat aaccaggaaa aacaagagct tatttgcaaa
    15961 ctggacaacc tggctttccc gctgcgcgat ggcattccag tgttgctgga aacggaagcc
    16021 cgcgtgctga ctgctgatga gagtaaatca tgagttttgt ggtcattatt cccgcgcgct
    16081 acgcgtccac gcgtctgccc ggtaaaccat tggttgatat taacggcaaa cccatgattg
    16141 ttcatgttct tgaacgcgcg cgcgaatcag gtgccgagcg catcatcgtg gcaaccgatc
    16201 atgaggatgt tgcccgcgcc gttgaagccg ctggcggtga agtatgtatg acgcgcgccg
    16261 atcatcagtc aggaacagaa cgtctggcgg aagttgtcga aaaatgcgcg ttcagcgacg
    16321 acacggtgat cgttaatgtg cagggtgatg aaccgatgat ccctgcgaca atcattcgtc
    16381 aggttgctga taacctcgct cagcgtcagg tgggtatggc gactctggcg gtgccaatcc
    16441 acaatgcgga agaagcgttt aacccgaatg cagtgaaagt cgttctcgac gctgaagggt
    16501 atgcactgta cttctcacgc gcaaccattc cgtgggatcg tgatcgtttt gcaaaaggcc
    16561 ttgaaaccgt tggcgataac ttcctgcgtc atcttggtat ttatggctac cgtgcaggct
    16621 ttatccgtcg ttacgtcacc tggcagccaa gcccgttaga acacatcgaa atgttagagc
    16681 agcttcgtgt tctgtggtac ggcgaaaaaa tccatgttgc tgtcgcgcat gaagttcctg
    16741 gcacaggtgt ggatacccct gaagatctcg agcgcgttcg cgctgaaatg cgctaatttc
    16801 acttcacgac actccagcca attttgggag gagtgtcgta ccgttacgat tttcctcaat
    16861 ttttcttttc aacaattgat ctcattcagg tgacatcttt tatattggcg ctcattatga
    16921 aagcagtagc ttttatgagg gtaatctgaa tggaacagct gcgtgccgaa ttaagccatt
    16981 tactgggcga aaaactcagt cgtattgagt gcgtcaatga aaaagcggat acggcgttgt
    17041 gggctttgta tgacagccag ggaaacccaa tgccgttaat ggcaagaagc tttagtacgc
    17101 ccggaaaagc ccgacaactg gcatggaaaa ccaccatgct ggcaagaagt gggactgtcc
    17161 gtatgccgac tatttatggt gtgatgacgc atgaagaaca ccccggccct gatgtcctgc
    17221 tactggagcg gatgcgtggt gtttcggtgg aggcaccagc ccgaacacca gaacgctggg
    17281 aacagctcaa agaccaaatc gttgaagcct tactggcctg gcaccgtcag gacagtcgcg
    17341 gttgcgtcgg cgcggtcgac aatactcagg aaaatttctg gccctcatgg taccggcaac
    17401 atgttgaagt gctatggacc acgctcaatc agttcaataa aaccggtctg acgatgcagg
    17461 ataagcggat cctgtttcgc actcgcgaat gtctcccggc attatttgaa ggctttaacg
    17521 acaattgtgt gttgattcac ggtaacttct gtttacgcag catgctgaaa gattcgcgca
    17581 gcgatcagtt actggcgatg gtcgggccgg gactaatgct ttgggcaccg cgagaatacg
    17641 aactgttccg actaatggat aattctctgg cggaagattt gctctggagt tacctgcaac
    17701 gcgcgccagt ggcggagtcg ttcatctggc ggcgttggtt gtatgtgtta tgggatgaag
    17761 ttgcgcaact ggttaatacc ggacgattta gtcggcgcaa cttcgatctg gcatcaaaat
    17821 cactcttgcc gtggctcgcc tgacgaacct ttcagccact gccagatgcg gccaagcgtt
    17881 tcatagccaa cacgatcgct atgcatcagc cagaccggag aagggattgc ccgttcccac
    17941 ggattaagcg gcgagtcgat ggccaactga ttcgccgggg ctggtagcgg atttaaccct
    18001 tcctgctgaa aaaagatcat cgcccgcggc agatgtgatg cggaggtcac cagcaggaaa
    18061 ggggcgtcac caatcgcctg tttcactgcc gcagcttctt cttcggtatc ttttggcaaa
    18121 tccagggtga taatttgctc gcgcggcacg cccagcgatt gcgcaactct ggcacctact
    18181 tccgctgtac tcaccgtatt ggtttttgct acgcctcccg tgaagatcag ttttgatccc
    18241 ggattttcgc gccataaacg gataccctca ttcaggcgtg gcagactgtt attgattaaa
    18301 ttagagctcg gtgcccactg cgggttccag gtataaccac ctccgagcac cacgatatag
    18361 tccacttttt gcgaattatt ccatgtcgga taggtgcttt cgatgggacg cagcaggcgg
    18421 tcggctaccg gttgcaaact caataacaag agtgccagcc acccaatgct aataaaaatc
    18481 ttcccggttt tttgaaaacg gctaaaccac agcagcgcca ggccagcgcc aataattaac
    18541 aacatcagcg gaagtggcag caacatattg ccaatcactt ttttcagtgt aaaaagcatc
    18601 ctttctggtt ccttttttaa ccatatagca agggatcgcc agcgatatta caccagacgg
    18661 gttcattctc gccgtggctg tgacaaaata gcgtctttat cagaaaacgt cttagccaac
    18721 cggtggagaa agcaatgcag gatcgcaatt ttgatgatat tgcggaaaag ttttcccgta
    18781 acatttacgg caccaccaaa gggcagcttc gacaggctat tctgtggcag gatctcgatc
    18841 gcgtgctggc ggaaatgggt ccgcaaaaac tgcgtgtgct ggatgcgggc ggtggagaag
    18901 ggcagaccgc aatcaaaatg gccgagcgcg ggcatcaggt cattttatgc gatctttctg
    18961 cgcagatgat cgaccgcgca aaacaggcgg cagaagcaaa aggtgtgagc gacaacatgc
    19021 aatttataca ttgcgccgct caggatgttg cttcgcattt ggaaacgccc gttgatctga
    19081 tattgttcca tgcggtgctc gagtgggtgg ctgatccccg cagcgtattg cagaccctgt
    19141 ggtcagtgtt gcgtccaggc ggcgtgttgt cgttaatgtt ctacaatgcg catggtttgt
    19201 tgatgcataa catggtcgcc gggaattttg attacgtgca ggcgggaatg ccgaaaaaga
    19261 aaaaacggac gctttcgcca gattatccac gcgacccggc gcaggtttat ctgtggctgg
    19321 aagaagctgg ttggcaaatt atgggtaaga caggcgttcg cgtgtttcat gattatctgc
    19381 gcgagaagca ccagcagcgc gactgctatg aagcattact tgaattagaa acgcgttatt
    19441 gccgtcagga accgtatatt accctggggc gttatattca tgtcaccgcg cgcaaaccgc
    19501 agagcaagga taaagtatga gtgaattttc ccagacagtc cccgaactgg ttgcctgggc
    19561 cagaaaaaat gacttctcca tctcgctgcc ggtagaccga ctctcttttc tgctggcggt
    19621 tgccacgctg aacggcgagc gtctggatgg tgagatgagt gaaggcgagc tggtggatgc
    19681 attccgccat gtgagtgatg cgtttgagca aaccagcgaa accatcggcg tgcgcgccaa
    19741 taacgcgatc aacgacatgg tgcgtcaacg tctgctgaac cgctttacca gcgagcaggc
    19801 agaagggaac gctatttacc gtctgacgcc gctcggcatc ggcattactg actattacat
    19861 ccgtcagcgc gagttttcta cgctgcgcct ttctatgcag ttgtcgattg tggcgggtga
    19921 gctcaaacgc gcagcagatg ccgccgaaga gggcggtgat gaatttcact ggcaccgtaa
    19981 tgtctatgcg ccactgaaat attcggtagc agaaattttc gacagtatcg acctgacgca
    20041 acgtctgatg gacgaacagc agcagcaggt gaaggacgat atcgcccagt tgctgaacaa
    20101 agactggcgg gcggcgattt ccagctgtga attgttgctt tcggaaactt ccggaacgct
    20161 gcgtgaattg caggatacac tggaagcggc aggcgacaaa ttgcaggcta atctgttgcg
    20221 cattcaggat gcgacgatga ctcatgacga tctgcatttt gtcgatcgtc tggtgttcga
    20281 tctgcagagc aaactcgacc gtattatcag ttggggccag caatccatcg acttgtggat
    20341 tggctacgac cgccacgtac acaaatttat tcgtaccgcg atcgatatgg ataaaaaccg
    20401 cgtctttgct cagcggttac gtcagtcggt acaaacctat tttgatgagc cgtgggcgct
    20461 aacttatgcc aatgccgatc gtctgctgga tatgcgtgac gaagagatgg cactgcgcga
    20521 tgaagaagtg actggggaac ttccgccaga tttggaatac gaagagttta acgagatccg
    20581 cgaacagctg gcggcgatca tcgaagaaca acttgctgtg tacaaaacca gacaagtgcc
    20641 gctggatctt ggtctggtgg tacgcgaata tctgtcacag tatccgcgtg cacgtcactt
    20701 tgacgttgcg cgcattgtta ttgatcaggc ggtacgtctt ggcgtagcgc aagcagattt
    20761 caccggactg ccagcgaaat ggcagccgat taatgattac ggagccaagg tacaggcgca
    20821 tgtcatcgac aaatattgaa caagtgatgc cggttaagct ggcgcaggcg ctggcgaatc
    20881 cgttatttcc ggcgctggac agcgccttac gttcaggccg ccatattggc ctcgacgaac
    20941 tggataatca tgcattcctg atggattttc aggaatatct ggaagagttt tacgcgcgtt
    21001 ataacgttga gcttattcgt gcaccggaag ggttcttcta tttacgtcca cgttccacca
    21061 cgctgatccc tcgttccgtc ttgtcggaac tggatatgat ggtcgggaaa atcctctgct
    21121 atctctatct cagcccggaa cggctggcga atgaggggat ttttactcaa caggaactgt
    21181 acgacgaact gctcaccctg gccgatgaag cgaaactgct gaaactggtg aacaaccgtt
    21241 caaccggttc agacgttgac cgtcagaagt tgcaggagaa agtacgttct tcgctcaacc
    21301 gtctgcgtcg tttaggcatg gtgtggttta tgggccacga cagcagcaag ttccgcatta
    21361 ccgaatcggt gttccgcttc ggagccgatg tgcgtgctgg cgacgatccc cgtgaagcac
    21421 agcgtcgcct gattcgtgat ggcgaagcaa tgccgattga aaatcatctg caactcaacg
    21481 atgaaaccga agagaatcag ccagatagcg gagaggaaga ataatgattg aacgcggtaa
    21541 atttcgctca ctgacgctga ttaactggaa cggctttttt gcccgaactt ttgaccttga
    21601 cgagctggtc acgacgcttt ctggcggtaa cggggcgggt aaatccacca ccatggcggc
    21661 gttcgttacg gcgctgatcc ccgacctgac cctgctgcat ttccgtaaca ctacggaagc
    21721 cggggccacc agcggttcgc gcgataaagg tctgcacggt aagctgaaag cgggtgtctg
    21781 ttattcgatg ctcgacacca ttaactcgcg ccaccagcgc gtggtggtcg gtgtgcgtct
    21841 gcaacaggtt gccggacgcg atcgtaaagt ggatatcaaa ccatttgcca ttcagggact
    21901 gccgatgtcg gtgcagccga cacagctggt gaccgaaact ctgaacgaac gccaggcgcg
    21961 cgtgctgccg cttaacgagc tgaaagacaa gctcgaggcg atggaaggcg tgcagtttaa
    22021 acagttcaac tccattaccg attaccactc gctgatgttc gatctgggca tcatcgcgcg
    22081 tcgtctgcgc tccgcatctg accgtagcaa attctatcgt ctgatcgaag cttcgctgta
    22141 tggcgggatc tccagtgcca ttacccgttc tctgcgcgac tacctgttgc cggaaaacag
    22201 cggcgtgcgt aaagcgttcc aggacatgga agcggcgctg cgtgaaaacc gcatgacgct
    22261 ggaagcgatt cgtgtcaccc agtcggaccg tgacctgttt aagcatctga tcagcgaagc
    22321 taccaactac gtggcggcgg actacatgcg tcacgccaac gagcgccgtg tccatctcga
    22381 caaagccctg gagtttcgtc gcgagctaca tacttcgcgt cagcaactgg cggctgagca
    22441 gtacaaacac gtcgatatgg cgcgtgagct ggcagagcac aacggtgccg aaggcgatct
    22501 ggaagcggat tatcagtcgg ccagcgatca cctgaacctg gtgcaaaccg cactgcgtca
    22561 gcaggagaag atcgaacgct acgaagcgga tctcgatgag ctgcagatcc gtctggaaga
    22621 gcaaaacgaa gtggtggcag aagccatcga acgccaggaa gagaacgagg ttcgtgcgga
    22681 agctgccgaa ctggaagtgg acgagctgaa aagccagctt gctgactacc agcaggcgct
    22741 ggacgtccag caaacgcgcg cgatccagta taaccaggcg attgctgcac ttaatcgtgc
    22801 caaagaactg tgccatctgc cggacttaac cgccgacagc gccgccgaat ggctggaaac
    22861 cttccaggcg aaagagcagg aagcgactga aaaaatgctc tctcttgagc agaaaatgag
    22921 catggcgcaa accgcgcaca gccagtttga gcaggcttat cagctggtgg tggcaatcaa
    22981 cggcccgctg gcgcgtaacg aggcgtggga tgtcgcccgc gaactgctgc gcgaaggggt
    23041 cgatcagcgt cacctggcag agcaggttca accgttgcgg atgcgcttaa gtgaactgga
    23101 acagcgtctg cgcgagcagc aagaagctga gcgtctgctg gcagatttct gcaaacgtca
    23161 gggcaagaat tttgatatcg atgaactgga agccctgcat caggagctgg aagcgcgtat
    23221 tgcctctctt tccgatagcg tgtctaacgc ccgcgaagag cgtatggcac tgcgccagga
    23281 gcaggaacag ctgcagtctc gcattcagag tttgatgcag cgtgcaccgg tttggctggc
    23341 agcgcaaaac agtctcaacc agttgagtga acagtgcggc gaagagttct cctccagcca
    23401 ggacgtcact gaatatctgc aacagttgct ggagcgtgag cgcgaggcga ttgttgaacg
    23461 cgatgaagta ggcgcgcgca aaaacgccgt cgatgaagag atcgaacgtt taagccagcc
    23521 tggcggctct gaagatcagc gtctgaacgc gctggcagag cgttttggtg gtgtgctgct
    23581 gtcagaaatt tatgacgacg ttagcctgga agatgcgcca tacttctcgg ctctgtatgg
    23641 tccgtcacgc cacgccatcg tggtgccaga tctgtcacag gtaaccgaac acctggaagg
    23701 cttgaccgat tgcccggaag atctctattt gatcgaaggg gatccgcagt cattcgatga
    23761 tagcgtgttc agcgttgatg agctggaaaa agcggtagtg gtgaaaatcg ccgatcgtca
    23821 gtggcgttat tcacgtttcc cggaagtgcc gctgtttggt cgtgctgcgc gtgaaagccg
    23881 tattgaaagc ctccatgccg agcgtgaagt gctttccgaa cgcttcgcca cgctctcctt
    23941 tgatgtacag aaaacccagc gtctgcatca ggcgttcagc cgctttatcg gcagtcatct
    24001 ggcggtggcg tttgagtctg acccggaagc agaaatccgt caactgaaca gccgtcgcgt
    24061 cgaactggag cgggcgttga gtaatcatga aaatgataac cagcagcagc gtattcagtt
    24121 tgagcaggcg aaagaaggcg ttacggcgct gaaccgcatt ctgccgcgtc tcaacctgtt
    24181 ggctgatgac agcctggcgg atcgcgtcga tgaaatccgc gaacgtctgg atgaagccca
    24241 ggaagccgcg cgttttgttc agcagtttgg caatcaactg gcgaaactgg aaccgatcgt
    24301 ttcggtattg cagagcgacc cggaacagtt cgaacagtta aaagaagatt acgcgtactc
    24361 tcagcagatg cagcgcgatg cccgtcagca ggcgtttgcc ctgacggaag tggtgcagcg
    24421 tcgtgcgcac tttagctatt ctgactcggc agaaatgctt agcggtaaca acgatctcaa
    24481 cgaaaaactg cgtgaacgtc tggaacaggc ggaagcggag cgtacccgcg cacgcgaagc
    24541 gttgcgcggt cacgcagcgc agttgagtca gtacaaccag gtgctggctt cgctgaaaag
    24601 ttcttacgac accaaaaaag agctactcaa cgatctgcaa cgtgaattgc aggatatcgg
    24661 cgtgcgtgct gatagcgggg cagaagagcg ggcacgtatt cgccgtgacg agttgcacgc
    24721 gcaactgagc aataaccgtt cgcgccgtaa tcaactggaa aaagccctta ctttctgcga
    24781 agcggagatg gacaacctga cccgcaaact gcgcaagctg gagcgggatt actttgagat
    24841 gcgcgagcag gtagtgaccg ccaaagcggg ctggtgtgcg gtgatgcgca tggtgaaaga
    24901 taacggcgtt gagcgccgct tacaccgtcg tgagctggct tatctctccg ctgatgattt
    24961 gcgttccatg tcggataagg cgttaggtgc gctgcgtctg gcggtggcgg ataacgaaca
    25021 tctgcgcgac gtgctgcgca tgtcggaaga tccgaaacgt ccggagcgta aaattcagtt
    25081 cttcgtggcg gtttatcagc atctgcgtga acgtattcgt caggatatta ttcgtaccga
    25141 tgatccggtg gaagctatcg aacagatgga gatcgaactt agccgtctga ccgaagaatt
    25201 aacctcccgt gaacagaaac tggcgatcag ttcccgcagc gtggcgaaca tcattcgcaa
    25261 aaccattcag cgcgagcaga accgtatccg tatgctcaac caggggttgc agaacgtatc
    25321 gtttggtcag gtgaacagcg tgcgcctcaa cgtgaacgtg cgtgaaacgc acgccatgct
    25381 gctggatgtg ctctctgaac agcatgagca gcatcaggat ctgtttaaca gcaaccgtct
    25441 gaccttctcg gaagcgctgg cgaaattgta tcagcgtctt aatccgcaga ttgatatggg
    25501 gcagcgcacg ccgcagacca ttggcgaaga actgctggat taccgcaact atctggaaat
    25561 ggaagttgag gttaaccgtg gttccgatgg ctggttgcgc gcagagtctg gtgcattgtc
    25621 gaccggtgag gcgattggta ccggtatgtc gattctggtg atggtggtac aaagctggga
    25681 agatgaatct cgccgcctgc gcggtaaaga tatctctcct tgccgcctgc tgttcctcga
    25741 tgaagcagcg cgactggatg ctcgttctat cgccacgctg tttgaattgt gtgaacgttt
    25801 gcaaatgcag ctcatcatcg cagcgccgga aaatatcagc ccggagaaag gcaccaccta
    25861 taaactggtg cgtaaagtct tccagaatac cgaacacgtt cacgtcgtcg gcctgcgagg
    25921 atttgcgccg caactccctg aaacgcttcc tggaagtgac gaagcgcctt ctcaggcgag
    25981 ttaaaatgaa ggcggcagca atgccgcctt ttctttttcc gaaaactccg tttctgcact
    26041 aaaaaaatgg cacataacgg cgcgcttttc tttaaacttc tttacattag gttatgtaaa
    26101 aacgtatcgg cgtttatata ctgaagataa gcctgatgag taacaggctt gctcgtcata
    26161 ctttcgtgag tattggcgtt gtacaggcaa gtcgtaaaat aacagcctgg ctattcagag
    26221 tatgataaaa acagggggca agggatgttg cttaatatga tgtgtggtcg tcggctgtcg
    26281 gcaatcagtt tgtgcctggc cgtaacattc gctccactgt tcaatgcgca ggccgatgag
    26341 cctgaagtaa tccctggcga cagcccggtg gctgtcagtg aacagggcga ggcactgccg
    26401 caggcgcaag ccacggcaat aatggcgggg atccagctat tgcctgaagg tgcggcagaa
    26461 aaagcccgca cgcaaatcga atctcaatta cccgcaggtt acaagccggt ttatcttaac
    26521 cagcttcaac tgttgtatgc cgcacgcgat atgcaaccca tgtgggaaaa ccgtgatgct
    26581 gttaaagcct tccagcaaca gctggcagag gtggcgattg ctggtttcca gccgcagttt
    26641 aataaatggg tagagttact gaccgatcct ggtgttaacg ggatggctcg cgacgtggtg
    26701 ctctctgatg cgatgatggg ctatctccat ttcattgcaa atattccggt caaaggcact
    26761 cgctggctat atagcagtaa accttatgcg ctttcaacgc cgccgctttc ggtgattaac
    26821 caatggcagc aggcgctgga taaaggtcaa ttgcctacgt ttgttgcagg actggcaccg
    26881 cagcatccgc aatatgcggc gatgcatgaa tcgttactgg ccttactcag tgacaccaaa
    26941 ccgtggcccc aactgaccgg caaagcaacg ttgcgcccag ggcagtggag taacgacgta
    27001 ccggcgttgc gcgaaatatt gcaacgcaca ggcatgttgg acggggggcc gaaaattact
    27061 ctacctggcg atgacacgcc aactgacgcg gtagtcagcc catccgctgt tactgttgaa
    27121 acagcagaaa ctaagccgat ggacaagcaa acgacgtctc gtagtaaacc tacgcctgcc
    27181 gttcgtgccg cctacgataa tgaactggtg gaagccgtta aacgttttca ggcatggcaa
    27241 ggattggggg cagatggtgc tattggcccg gcaacgcggg actggttaaa cgtaacgccc
    27301 gcccagcgtg ctggcgtgtt ggcactcaac atccagcgat tgcgcttgct gccaacagag
    27361 ctttctaccg ggatcatggt taacattccg gcctattcgc tggtctacta tcagaacggc
    27421 aatcaggtgc tggattcgcg agtcattgtc ggtcgccccg atcgcaaaac gccgatgatg
    27481 agcagtgcac ttaacaacgt agtggtaaac ccgccgtgga acgtacctcc aactctggca
    27541 cgcaaagata ttctgccgaa agtgcgcaac gatccgggat atctcgaaag ccatggctat
    27601 acggtgatgc gcggctggaa cagcagagaa gcgattgacc catggcaggt tgactggtct
    27661 acaatcacgg cctcgaattt accgtttcgc ttccagcagg ctccaggccc acggaactcg
    27721 ctggggcgct ataaattcaa tatgccgagt tcagaggcca tttatttgca tgacacgccg
    27781 aaccacaatc tgttcaagcg tgatacacgc gcattgagct caggctgtgt acgagtgaat
    27841 aaagcttccg atctggcgaa tatgctgttg caggatgcag gctggaatga caaacgtatt
    27901 tctgatgcgc tgaagcaggg tgatacacgt tacgtcaata ttcggcagtc gattccggtg
    27961 aatctctact acctgacggc ctttgttggt gcagatggtc gtacccagta tcgtacagat
    28021 atttacaatt atgatctgcc tgcgcgatcc agctcgcaaa tcgtatcgaa agcggaacaa
    28081 ttaatcaggt aaatgaagta gttcggggaa atcagttgtc gtaataatta gcatgattgg
    28141 gggcgattct ctgcagcccc cgtcactgct ggggttgagt catcttgacg tctgctttac
    28201 gggcggttaa ggtgcctctt gtgcgccaga agtgcatata aacgataaca ttgacctgta
    28261 gacttgatta tcatggacaa attcgacgct aatcgccgca aattgctggc gcttggtggc
    28321 gttgcactcg gtgccgccat cctgccgacc cctgcgtttg caacactctc taccccacgc
    28381 ccgcgcattt tgacactcaa taatcttcat accggagagt caatcaaagc ggagtttttc
    28441 gatggcagag gctatattca ggaagaattg gcaaaactta accatttttt ccgcgattac
    28501 cgcgcgaaca aaataaagtc catcgaccca ggattattcg accagttgta tcgcctgcaa
    28561 gggttgttag gcacgcgcaa accggtgcaa ctcatttccg gttatcgttc cattgatacc
    28621 aacaatgaac tacgcgcccg cagccgtgga gtagcgaaga aaagctatca cactaaaggc
    28681 caggcgatgg atttccatat tgaaggtatc gcgttaagca atattcgcaa agccgcgtta
    28741 tctatgcgcg caggtggtgt aggatattac ccacgtagta actttgtgca tattgatacc
    28801 gggccagcac ggcactggta gtaattgctt aacgaaacag gggcagtatg aactatcgta
    28861 ttattccggt caccgcattc tcccagaact gttcattgat ctggtgtgaa caaacccgcc
    28921 tggccgcact ggtcgatcct ggcggcgatg cggaaaaaat caaacaggaa gttgatgaca
    28981 gcggcctgac actgatgcag atcctgctga cgcatggtca tctggaccac gttggcgcag
    29041 cggcggaact ggcgcaacat tacggcgtgc cggtttttgg tccggaaaaa gaagatgagt
    29101 tctggctgca aggcttgcct gcgcaaagtc gtatgtttgg tctggaagag tgccagccgc
    29161 tgacgccaga tcgttggctg aacgaaggcg ataccatcag catagggaat gtgactttac
    29221 aggtgttaca ttgccctgga catacgccgg gtcatgtcgt gttttttgat gatcgggcaa
    29281 agctgctgat ttctggcgat gttattttca aaggcggagt agggcgcagt gacttcccgc
    29341 gtggcgatca taatcaactg atttcttcaa tcaaagataa attgttgcca ctgggggatg
    29401 acgtgacatt tattccgggt cacggaccat tatccacact tggttatgaa cgcctgcata
    29461 atcccttcct gcaagacgaa atgcccgtct ggtaaggcac ataaaaaagc ccgcttttaa
    29521 tgcgggcctg gatttctggc aaagtgcgct ttgtttatgc cggatgcggc acgagcgcct
    29581 tatccggcct acaaaatcgt gcaaattcaa aatattgcag gggacgcgta ggcctgataa
    29641 gcgtagcgca tcaggcaatg ttgcgtttgt catcagtctc agcccgcttt tcagcgggct
    29701 tcattgtttt taatgcttac agcactgcca caatcgcttc gcacagcgga gccatgttat
    29761 ctggtgtcat ccctgccaca tttacgcgac cagaagcaac agcatatacg ccaaactctt
    29821 cgcgcagacg cagcacttgt tcttttgtca ggccactgaa ggagaacatg ccgttctgtt
    29881 tgatgataaa gctgaagtcg cggtttgccc ctttttcctg cagcgtattg acgaacaact
    29941 gacgcatacg ctgaatacgc tggcgcatat cagtcagctc ttgttcccaa atcgcacgta
    30001 acgcatcgtt gctcaggatg gtggcaacaa cagaagcgcc gtgtgctggt gggttagagt
    30061 agttagcgcg aatcgccgct ttcatttggc tgaatgcgcg atcaacggtt tcactgtcag
    30121 cagcaaccag agtacaagcg ccaacacgct cgttgtacag gccaaagttt ttagagtagg
    30181 aactggcaac aatcagctct ttatgcatag ccgcgaaagc gcgcagtcct tcagcatctt
    30241 cttccagacc acgggcaaac ccctggtaag cgaagtcaaa cagcggtaac cagcctttct
    30301 caacggagag ttgtgccagt gtctgccatt gttccagcgt agggtcgata ccggttgggt
    30361 tgtggcagca gccatggaac agcactacgt cgccagcctg agcttcgttc aggctgttaa
    30421 tcagtgcatc gaagtcaagg gtgtggtttt ccgcatcata ataagcgtat tcacgaactt
    30481 ccagacctgc agagttaaag acgctcttat ggttcggcca gcttgggttg ctcacccaca
    30541 cacgcttaac gctggtattt tttgccagga aatcggcagc cacgcgtagt gcgccagtgc
    30601 cacccggagt ctgtgccgtg agagcacgtt tgtcattgat cagggcgcta cctttaccaa
    30661 acagcagttc ctgagtgcag cgaccaaatt cagggatgcc gtcaatgccg aggtaatttt
    30721 tggtggtttc attttcgagc agatactgtt cagccttttt cacgctggtc agtaccgggg
    30781 ttttgcccgt ctcatcttta tagacaccaa tcccgaggtt aattttgccg ggacgttcat
    30841 cggcacgaaa cagatcggcc aggcccagaa tcgggtcggc aggagcggcg gtaatgttct
    30901 caaacatgac gaggttccat tatggttaca gaagggaagt ccgctatcag ggtaacggga
    30961 gatttacaaa attccaacta ttactgatga aaacgcaggc tgtttttgca agacgtgaga
    31021 ttgctctgga aggcataaaa aaaacaggac caaagtcctg ttttttcggc atttaacaaa
    31081 gaggtgtgct attagaactg gtaaacgata cccacagcaa cggtgtcgtc tgaacctacg
    31141 cccagtttgt tgtcagaatc gatctggttg atgatgtagt caacataggt ggacatgttt
    31201 ttgttgaagt agtaggttgc gcccacttca aagtagttca ccagatcaac atcaccgata
    31261 ccttctacgt ctttcgcttt agatttggtg taagcgatgg acggacgcag accgaaatcg
    31321 aactggtatt gcgcaactaa cagaacgtct tgcgttttgt tggcgaagcc gccttcaatt
    31381 ggcgtagcgt tacgggtttc accgtagttc gctgccagat agatgttgtt cgcgtcatac
    31441 ttcagaccag ttgcccactg ttcagctttt ttaccctgac cgcggaattc ggcttcctgg
    31501 gcgtcggtac ggtctgctgc accgtaagca cctacgatac caaagccttc gaattcgtag
    31561 ctgatagagc caccgacgcc gtcgccgttg gaacgctcag gaataccagc acgttcgttt
    31621 ttgcccaggt actgaacggc aaagttcagg ccgtcaacca gaccgaagaa gttggagtta
    31681 cgataggtag caacgccgcc tacacgacca acgaagaagt catcgctgta tgccgtgtca
    31741 ccgccaaact caggcagcat atcggtgtaa cccagtgcgt cgtaaaccac accgtagtta
    31801 cgaccgtagt cgatagaacc cgcatcagcg aatttcagac ctgcgaatgc cagacgagtt
    31861 ttgttacctt tctgggcatc agcgccttca gagttgttac cctggaagtt atattcccac
    31921 tgaccgtagc cggtcagatc ggaattgatt tgtgtttccc ctttaaaacc aagacgggca
    31981 taagttttgt cgccgtttcc gccgtaactg tttacaccat tgtctttaga aaaataatgc
    32041 agaccgacag ctttaccgta cagatctact ttgttgccat ctttgttata gatttctgca
    32101 gcgtttgcag tacctgctac taacagagca gggacgatca ctgccagaat attgcgcttc
    32161 atcattattt attaccctca tggttttttt tatgacacct gccactgccg tcaataattt
    32221 ctgtcaataa aaatgtacgg aactattgat gagagtttgg tgtctttatg tgtctgcagg
    32281 catctttcca ttcaaactaa cgtttc
//
LOCUS       DAPGOF010000041        32265 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.041, whole genome shotgun sequence.
ACCESSION   DAPGOF010000041 DAPGOF010000000
VERSION     DAPGOF010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.041"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            93..1382
                     /locus_tag="RHV13_001632"
     CDS             93..1382
                     /locus_tag="RHV13_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HDV1241181.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            1379..2845
                     /gene="hpaE"
                     /locus_tag="RHV13_001633"
     CDS             1379..2845
                     /gene="hpaE"
                     /locus_tag="RHV13_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HDV1241182.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            2847..3698
                     /gene="hpaD"
                     /locus_tag="RHV13_001634"
     CDS             2847..3698
                     /gene="hpaD"
                     /locus_tag="RHV13_001634"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HDV1241183.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGIYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            3708..4088
                     /locus_tag="RHV13_001635"
     CDS             3708..4088
                     /locus_tag="RHV13_001635"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HDV1241184.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            4156..4959
                     /gene="hpaH"
                     /locus_tag="RHV13_001636"
     CDS             4156..4959
                     /gene="hpaH"
                     /locus_tag="RHV13_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HDV1241185.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            4970..5758
                     /gene="hpaI"
                     /locus_tag="RHV13_001637"
     CDS             4970..5758
                     /gene="hpaI"
                     /locus_tag="RHV13_001637"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HDV1241186.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            5881..7257
                     /gene="hpaX"
                     /locus_tag="RHV13_001638"
     CDS             5881..7257
                     /gene="hpaX"
                     /locus_tag="RHV13_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HDV1241187.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLIQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            7267..8157
                     /gene="hpaA"
                     /locus_tag="RHV13_001639"
     CDS             7267..8157
                     /gene="hpaA"
                     /locus_tag="RHV13_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HDV1241188.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPSVPHAFITESDADGHVLTVRED
                     LIWPLLEVLYPGTRETFGLPGICLSLADKPDELAALEHYWQLIERESVEQLPGREHTL
                     TLLAQAVFTLLLRNAKLDDHAASGMRGELKLFQRFNMLIESHFHQHWTVPDYANELHI
                     TESRLTDICRRFANRPPKRLIFDRQLREAKRLLLFSDNAVNNIAWQLGFKDPAYFARF
                     FNRLVGCSPSAYRAKKVPVT"
     gene            8408..9970
                     /gene="hpaB"
                     /locus_tag="RHV13_001640"
     CDS             8408..9970
                     /gene="hpaB"
                     /locus_tag="RHV13_001640"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HDV1241189.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            9988..10500
                     /locus_tag="RHV13_001641"
     CDS             9988..10500
                     /locus_tag="RHV13_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HDV1241190.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            10893..13043
                     /gene="btsT"
                     /locus_tag="RHV13_001642"
     CDS             10893..13043
                     /gene="btsT"
                     /locus_tag="RHV13_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HDV1241191.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            13093..13296
                     /locus_tag="RHV13_001643"
     CDS             13093..13296
                     /locus_tag="RHV13_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HDV1241192.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            13307..14263
                     /gene="yjiA"
                     /locus_tag="RHV13_001644"
     CDS             13307..14263
                     /gene="yjiA"
                     /locus_tag="RHV13_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HDV1241193.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDETPHSTMVFIGIQLPEDEIRAAFAGLRK"
     gene            complement(14309..15223)
                     /gene="mrr"
                     /locus_tag="RHV13_001645"
     CDS             complement(14309..15223)
                     /gene="mrr"
                     /locus_tag="RHV13_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine/cytosine-specific restriction
                     endonuclease Mrr"
                     /protein_id="HDV1241194.1"
                     /translation="MTVPTYDKYIEPLLRYLATKPEGAAARDVHEAAADALGLDDSQR
                     AKVITSGQLVYKNRAGWAHDRLKRAGLSQSLSRGKWCLTPAGFDWVASHPQPMTEQET
                     NHLAFDFVNVKLKSRPDAVDLDPKADSPDHEELAKSSPDDRLDQALKELRDAVADEVL
                     ENLLQVSPSRFEVIVLDVLHRLGYGGHRDDLQRVGGTGDGGIDGVISLDKLGLEKVYV
                     QAKRWQNTVGRPELQAFYGALAGQKAKRGVFITTSGFTSQARDFAQSVEGMVLVDGER
                     LVHLMIENEVGVSSRLLKVPKLDMDYFE"
     gene            15411..18923
                     /gene="hsdR"
                     /locus_tag="RHV13_001646"
     CDS             15411..18923
                     /gene="hsdR"
                     /locus_tag="RHV13_001646"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system
                     endonuclease"
                     /protein_id="HDV1241195.1"
                     /translation="MMNKSNFEFLKGVNDFTYAIACAAENNYPDDPNTTLIKMRMFGE
                     ATAKHLGLLLNIPPCENQHDLLRELGKIAFVDDNILSVFHKLRRIGNQAVHEYHNDLD
                     DAQMCLRLGFRLAVWYYRLVTKDYDFPVPVFVLPERGENLYHQEVLTLKQQLEQQVRE
                     KAQTQAEVEAQQQKLVALNGYIAILEGKQQETEAQTQARLAALEAQLAEKNAELAKQT
                     EQERKAYHKEITDQAIKRTLNLSEEESRFLIDAQLRKAGWQADSKTLRFSKGARPEPG
                     VNKAIAEWPTGKDETGKQGFADYVLFVGLKPIAVVEAKRKNIDVPGKLNESYRYSKCF
                     DNGFLRETLLEHYSPDEVHEAVPEYETSWQDSSGQQRFKIPFCYSTNGREYRAAMKTK
                     SGIWYRDVRDTRNMSKALPEWHRPEELLEMLGSEPQKQNQWFADNPGMSELGLRYYQE
                     DAVRAVEKAIVKGQQEILLAMATGTGKTRTAIAMMFRLIQSQRFKRILFLVDRRSLGE
                     QALGAFEDTRINGDTFNSIFDIKGLTDKFPEDSTKIHVATVQSLVKRTLQSDEPMPVA
                     RYDCIVVDEAHRGYILDKEQTEGELQFRSQLDYVSAYRRILDHFDAIKIALTATPALH
                     TVQIFGEPVYRYTYRTAVIDGFLIDQDPPIQITTRNAQEGVYLSKGEQVERISPQGEV
                     INDTLEDDQDFEVADFNRGLVIPAFNRAVCNELTNYLDPTGSQKTLVFCVTNAHADMV
                     VEELRTAFKKKYPQLEHDAIIKITGDADKDARKVQTMITRFNKERLPNIVVTVDLLTT
                     GVDIPSICNIVFLRKVRSRILYEQMKGRATRLCPDVNKTSFKIFDCVDIYSTLESVDT
                     MRPVVVRPKVELQTLVNEITDSETYKITEADGRSFAEHSHEQLVAKLQRIIGLATFNR
                     DRSETIDKQVRRLDELCQDAAGVGFNGFASRLREKGPHWSAEVFNKLPGFIARLEKLK
                     TDINNLNDAPIFLDIDDEVLSVKSLYGDYDTPQDFLEAFDSLVQRSPNAQPALQAVIN
                     RPRDLTRKGLVELQEWFDRQHFEESSLRKAWKETRNEDIAARLIGHIRRAAVGDALKP
                     FEERVDHALTRIKGENDWSSEQLSWLDRLAQALKEKVVLDDDVFKTGNFHRRGGKAML
                     QRTFDDNLDNLLDKFSDYIWDELA"
     gene            19124..20713
                     /gene="hsdM"
                     /locus_tag="RHV13_001647"
     CDS             19124..20713
                     /gene="hsdM"
                     /locus_tag="RHV13_001647"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system
                     methyltransferase"
                     /protein_id="HDV1241196.1"
                     /translation="MNNNDLVAKLWKLCDNLRDGGVSYQNYVNELASLLFLKMCKETR
                     QEAEYLPEGYRWDDLKSRIGQEQLQFYRNLLVHLGADNQKLVQAVFQNVNTTITQPKQ
                     LTELVSNMDSLDWYNGAHGKSRDDFGDMYEGLLQKNANETKSGAGQYFTPRPLIKTII
                     HLLKPQPREVVQDPAAGTAGFLIEADRYVKSQTNDLDDLDGDTQDFQIHRAFIGLELV
                     PGTRRLALMNCLLHDIEGNLDHGGAIRLGNTLGSDGENLPKAHIVATNPPFGSAAGTN
                     ITRTFVHPTSNKQLCFMQHIIETLHPGGRAAVVVPDNVLFEGGKGTDIRRDLMDKCHL
                     HTILRLPTGIFYAQGVKTNVLFFTKGTVANPHQDKNCTDDVWVYDLRTNMPSFGKRTP
                     FTDEHLQPFERVYGEDPHGLSPRTEGEWSFNAEETEVADSEENKNTDQHLATSRWRKF
                     SREWIRSAKSDSLDISWLKDKDSIDADNLPEPDVLAAEAMGELVQALGELDALMRELG
                     ASDEADAQRQLLEEAFGGVKE"
     gene            20710..22023
                     /locus_tag="RHV13_001648"
     CDS             20710..22023
                     /locus_tag="RHV13_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDV1241197.1"
                     /translation="MSKNWQICQLGDVLTLINGRAYKKEEMLTEGVPILRIQNLNGGN
                     KWFYSNLELQEDKYCYKNDLLYAWSATFGPYWSKWDHKLIYHYHIWKIETSPALNKQF
                     AYYALLNITEALKASAHGVAMPHITKAGMEAWEIQLPPISEQKIIAEKLDTLLAQVES
                     TKARLEQIPQILKRFRQAVLGAAVSGKLTEDWRDNSSLSGWIEGKLGEFIKKPSYGTS
                     SKSNKEGLIPVLRMGNLQGGKLDWTDLVYTSDTIEIEKYKLEYNDVLFNRTNSPELVG
                     KTAIYKSEQPAIYAGYLIRVQCLPDLNPDYLNYHLNSILGRQYCYSVKSDGVSQSNIN
                     AQKLIAYPITVPPLPEQHEIVRRVEQLFAYADTIEKQVNNALARVNNLTQSILAKAFR
                     GELTAQWRAENPELISGENSAAALLEKIKAERAASGGKKASRKKS"
     gene            22251..22592
                     /gene="symE"
                     /locus_tag="RHV13_001649"
     CDS             22251..22592
                     /gene="symE"
                     /locus_tag="RHV13_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HDV1241198.1"
                     /translation="MTDTYSIAQPFEAEVSPANNRHVTVGYASRYPDHAHIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAEESELMQSLRRVCKLSARKQKQVQEF
                     IGVIAGKQKVA"
     gene            complement(22635..28979)
                     /locus_tag="RHV13_001650"
     CDS             complement(22635..28979)
                     /locus_tag="RHV13_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDV1241199.1"
                     /translation="MTTRYFSSLIEQSLSRSTEATLSIMGVTNPQLREHLAQQMGADC
                     GKPGSFLASPVFQQMFGWKESNHTMRSLTEGNALLSKAVVDSLDNQSNGRYRFGADWK
                     PFTHQLASWKALLEKKHSVVVTSGTGSGKTECFMVPVLEDLYRELHENGNNPLVGVRA
                     LFLYPLNALINSQRERLDAWTRGFGTGIRYCLYNGNTEELHAKMKTVQDNRPNEVLSR
                     EKMRLEPAPILVTNGTMLEYMMVRQVDAPIIQQSKAQKSLRWIVLDEAHTYVGSQAAE
                     LALQLRRVMTAFGVTPDDVRFVATSATIAGSDAEKQLKKFLSELSGIPQERIDVLDGS
                     RVIPELEPSKNVSVPLDEIEQIPDLDLKDKNDNKIKGISPERFYALTHSPEARYIREM
                     LVKHPNPMKLDEMTERLNILTKQHHSQHEVLRWIDVCSGTQPNAKDPAFLKVRAHIFQ
                     RNTQGIWACVDKDCRLKHGTPLEKGWPFGYVYVNQRQNCDCGSPVYEVAFCNDCNEPH
                     LLARDKKGKLVQWENKGGDEFSLQDEVPVEHDATAEKVEKENSFQPPLIIAAGETSEA
                     GYTLQRLDRQTRRIGVINNDSIPLIINDVEQVCSASGCGYRGMSGKQPFRRALLGGPF
                     YVTNIVPTVLEYCQDFTSDEGKEGVGPDSLPGRGRRLITFTDSRQGTARMAVRMQQEA
                     ERSRLRGSVVEILSWHQRTQTSTAPNANADLEKLATRAKQAREQAEEYRSWGMPDQAK
                     LSQAQAEQLEQAYQFAIGGKAATTLVSRTWTEMVNELKDKADIRGPVLKYNYYLKPEV
                     FNENGGPLKLSEMLLFREFMRRPKRTNSLETQGLVQVGYLGLEKIHKLPMHWQERELT
                     LDDWRDFLKVTLDHYVRESNFTQLDDELKNWIGSRFSSKFVRNPESKDPEDNQNRRWP
                     QIRNGNVSHRLAKLLMLGAGFKTVNTATIDIINTWLKEAWAQLTGPLAVLKPDGNRFY
                     LPKEHMTFSLITDAWICPVTNKILDTAFKGLTPYLPTHISFEHLTQAQYDTFVAQKVT
                     MPEIWKLDRSQEDYAEGLAKARDWVSNDPLIAQLRSENVWTDINDRVVEGGFYYRTAE
                     HSAQQSSERLQSYEKMFKNGQLNVLNCSTTMEMGVDIGGITAVVMNNVPPHPANYLQR
                     AGRAGRSKESRAISYTLCKGNPHDQQVFANPLWPFETMIPAPMVAMNSARLVQRHVNA
                     LLLSDFLCNVIGETDKEKTSLDSLWFFGEDDGQSKCERFKIWLERPVLDIDTALERLV
                     KGTALHGARAEHLRDKTISAITFLQQRWLSVYRDLVTQERESQPQTPYRKRIELEKKR
                     HCGEYLLRDLAARTFLPGYGFPTDVVTFDNFTMEDYIREKLQKSRDKKDREDNVSRYK
                     GLPSRNLGVAIREYAPGAEIILDGRVFRSAGVSLHWHNINADTNEAQRLDCAWRCHKC
                     GTIGYEEGMSSSGMLFCSNSACGEKIIMDNRRQVLQPAGFVTDAHAPVTNNIETMKFV
                     PVVPAWVFVKAEPVPLPNPLMGYMASGADGHVFQQSLGEGGHGYALCLSCGRAESMLN
                     ENDAPKSMEAHYPPRPGKADRDSQNHRLICPGSTALMKNVTLGALARTDVFEMVLRKP
                     QKGEYLPDNTEEGRIVAMTLAVALRQALAGVLGISGAELGYSVRPVRLEDGQSVLAVQ
                     LYDVISGGAGFASSAPVHIEAILQGMVKQLGCRHCDTACSECLLDSQTRHDHDLLDRK
                     AALAWLGDDFTYYIGLPDEETFSLPDARYCPGAIGDTIRRAINEGAEKLTLWMTGAPN
                     EWDLYARQFRAAVQSYRLKDNVEVDLVIPAGVDDPDLLYELSQFTALGVRLCHVEQDL
                     QLPIVAQVTFADRVMTLASRSQQATIPGPEWHLNDELVVRSLGYKTVELNEFILPAKA
                     ANAVERVKDIQIHKQLNGPLSQFGQRFWDVLFNDHEEAQSLMNNTRITGVHYTDRYLQ
                     NPVALALLGSILRPLKTKLTDVAEVALDTLFKDKDRPGNRPFHDWMSIADFQDFADQW
                     FAAALGRPIELTVFDSPRDIPHHRKLTVTFEDGQVLKIRFDQGMGYWRINFASQWHYF
                     DFRDDVSFQLVKMAQACNEGNVANSEESWATDVLVEVSAS"
     gene            complement(28992..31196)
                     /locus_tag="RHV13_001651"
     CDS             complement(28992..31196)
                     /locus_tag="RHV13_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000635256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1241200.1"
                     /translation="MIVGSIKLDDSEIAIGEVPLTFIVDADQWLLQGQASCSVQSSDV
                     LIVLPRDNSTVAGFDGQSSAVNVLGLKALPVKGCQDVTVTANETYRIRTGREQISIGR
                     FALNGKRASWVCHPDETFIGVPKVISTLPDIQSIDVTRYLSGISIEQCHIQEMLGAQY
                     LSIRNSNNETLLRRKIGILPADFSIEIKGGMHANEGTIVITTQHPCVAKLKDKTLEAT
                     RKRTEGRTEIQLKAEGVPPAFITLQVLPNLAADPIDIELPFPAKGCLALDVNGRPLDK
                     NITLHDLLGSRAFLFSRNGEPTKYTLQLYLRSVSGLQAWHEWCYTALSDRPVELNLYS
                     LREHIENLISLEAGIDQVVEMRITGAGVVMAWQIRRYKYSLRYDYEKELLLSQSVNHR
                     AGQIPSPVIMLLSEPERKSIPLASRMSEGVPVGEYELSSIVNKNGPWLVVPKPGEEMA
                     FRPCFIRGESSLPVEESNIRSLQKATQLFNPQAEVNTITRVLGQMANDPAHSGWQFMR
                     SLYDQFGYLPLATFEVWRALVQHPQALAMSLFKFEMSAEYLSRIENEFPILWEFFPIF
                     EIKAASERFKLFLSQKGAPEETQKLLVTNMFQRLGLVFPTYADEIEKWLSNGYLPPSI
                     PESCVHGWYQELLREHSEARWPEYGCKRLYEWMMSQKNPVIGINPDADYRYSVAWLPV
                     FAAAVASGNASFESVFDRKPGAVFFLRQVRDFDSRWFKAIFQCSLLRYVAKK"
     gene            complement(31418..32265)
                     /locus_tag="RHV13_001652"
                     /pseudo
     CDS             complement(31418..32265)
                     /locus_tag="RHV13_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367038.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         7913 a         8614 c         8263 g         7475 t
ORIGIN      
        1 tacatcactt acttttgcca aactacgcaa tcgggaataa aagtaatcat taacatatta
       61 atgattaaaa tgatcgaatc cgaggagtgg taatgaaagg cactatcttc gccgtagcgt
      121 tgaaccatcg cagccaactt gatgcatggc aggaagcgtt ccagcaatcc ccctacaaag
      181 ccccgcctaa aactgcggtc tggtttatta aaccgcgcaa tacggtgatt ggttgcggtg
      241 aaccgattcc ctttccacag ggtgaaaagg tactgagcgg tgcgactgtt gcgctgattg
      301 tgggaaaaac ggcgacgaaa gtacgtgaag aagatgcggc agagtacatc gccggatatg
      361 cgctggctaa cgacgtcagc ctgccggaag agagctttta ccgcccggca atcaaagcaa
      421 aatgtcgtga tggattctgc cccattggcg aaaccgtggc tctcagcaat gtcgataatc
      481 tgaccatcta taccgagatc aacgggcgtc ctgccgatca ctggaatacc gccgatttac
      541 aacgtaacgc cgcgcagttg ctgagcgccc tgagcgaatt tgccacgctg aatccaggcg
      601 acgccattct gctcggcacg ccacaggcgc gcgtggaaat acagccaggc gatcgcgttc
      661 gtgttctcgc agaaggtttc ccgccgctgg aaaatccggt agtggacgaa cgtgaagtga
      721 ccacgcgcaa gagcttccca acgcagccac acccgcacgg cacgctgttt gccctcggcc
      781 tgaactacgc cgaccacgcc agcgaactgg aatttaagcc accggaagaa ccgctggtgt
      841 tcctgaaagc gccaaatacc ctcactggcg ataaccagac ctccgtgcgt ccaaacaata
      901 ttgaatacat gcactatgaa gcggagctgg tggtagttat tggcaaacag gcgcgtaacg
      961 tcagcgaagc cgatgccatg gattatgtcg cgggctacac cgtgtgtaac gactacgcca
     1021 ttcgcgacta tctggaaaac tactaccgcc ctaacctgcg ggtcaaaagc cgcgacggac
     1081 tgacgccgat gctttcaacc atcgtgccga aagaggcgat cccggacccg cataatctga
     1141 cccttcgcac cttcgtcaac ggcgagttac gccagcaagg caccaccgcc gatctgatct
     1201 tcagcgtgcc cttcctgatc gcctacttaa gcgaatttat gaccctgaat ccgggcgaca
     1261 tgatcgccac cggcacacca aaaggcttat ctgacgtggt gcctggcgat gaagtagtgg
     1321 tggaagtaga aggcgtgggc cgcctggtga accgaattgt gagtgaggaa acagcgaaat
     1381 gaaaaaagta aatcattgga tcaacggcaa aaatgttgca ggtaacgact acttccagac
     1441 caccaatccg gcaacgggtg aagtgctggc ggatgtggcc tctggcggtg aagcggagat
     1501 caatcaggcg gtagcggcag cgaaagaggc gttcccgaaa tgggccaatc tgccgatgaa
     1561 agagcgtgcg cgcctgatgc gccgtctggg cgatctgatc gaccagaacg tgccagagat
     1621 cgccgcgatg gaaaccgcgg acaccggcct gccgatccat cagaccaaaa atgtgttgat
     1681 cccacgcgct tcccacaact ttgaattttt cgcggaagtc tgccagcaga tgaacggcaa
     1741 gacctatccg gttgacgaca agatgctcaa ctacacgctg gtgcagccgg tgggcgtttg
     1801 tgcgctggta tcgccgtgga acgtaccgtt tatgaccgcc acatggaagg tcgcgccgtg
     1861 tctggcgctg ggcaataccg cggtactgaa aatgtcggaa ctctccccgc tgaccgctga
     1921 ccgcctgggt gagctggcgc tggaagccgg tattccggca ggcgtgctga acgtggtaca
     1981 gggctacggc gcaaccgcag gggatgcgct ggttcgtcat catgacgtac gtgccgtgtc
     2041 gttcaccggc ggtacggcca ccgggcgcaa catcatgaaa aacgccgggc tgaaaaaata
     2101 ctccatggaa ctgggcggta aatcgccggt gctgattttt gaagatgccg atattgaacg
     2161 cgcgctggac gccgccctgt tcaccatctt ctcgatcaac ggcgaacgct gcaccgccgg
     2221 ttcgcgcatc tttattcagc aaagcatcta cccggaattc gttaaacgct ttgccgaacg
     2281 cgccaaccgt ctgcgcgtgg gcgatccgaa cgatccgaat acccaggttg gcgcgcttat
     2341 cagccagcag cactgggaaa aagtctccgg ctatatccgt ctcggcattg aagaaggcgc
     2401 aaccctgctg gcgggcggcc cggataaacc gtccgacctg cctgcgcacc tgaaaggcgg
     2461 caacttcctg cgcccaaccg tgctggcaga cgttgataac cgtatgcgag tcgcccagga
     2521 agagattttc gggccggtcg cctgcctgct gccgtttaaa gacgaagccg aaggcttacg
     2581 tctggcaaac gacgtggagt acggcctcgc gtcgtacatc tggacacagg atgtcagcaa
     2641 agtgttacgc ctggcgcgtg gcattgaagc tggcatggtg ttcgtcaaca cccagaacgt
     2701 gcgtgacctg cgccagccat ttggcggcgt aaaagcctcc ggcaccgggc gtgaaggcgg
     2761 tgagtacagc ttcgaagtgt tcgcggaaat gaagaacgtc tgcatttcca tgggcgacca
     2821 tccaattccg aaatggggag tctgatatgg gtaagttagc gttagccgcc aagatcacgc
     2881 acgtaccgtc gatgtatctc tctgaactgc cagggaaaaa ccacggttgc cgccagggcg
     2941 cgatcgacgg gcataaagag atcagcaagc gttgccggga aatgggcgtc gataccatta
     3001 tcgttttcga tacccactgg ctggtcaaca gtgcttatca catcaactgt gcagaccatt
     3061 ttgaaggcat ctacaccagc aacgagctgc cgcattttat ccgcgatatg acctacaact
     3121 acgagggcaa cccggagttg gggcagctta ttgccgatga agccttaaag ctcggcgtgc
     3181 gggcaaaagc gcacaacatt cccagcctga aactggaata cggcacgctg gtaccgatgc
     3241 gctacatgaa tgaagacaag cacttcaaag tggtttccat ttcagctttc tgcactgttc
     3301 acgattttgc cgacagccgc aagctgggcg aggcgattct gaaagcgatc gaacagtacg
     3361 acggcaccgt ggcggtcctt gccagcggtt cgttatcgca ccgctttatt gacgatcagc
     3421 gagcggaaga agggatgaac agctacaccc gcgagttcga ccgccagatg gacgagcgtg
     3481 tggtgaagct gtggcgcgaa ggccagttca aagagttctg caatatgctg ccggagtacg
     3541 ccgactactg ctacggcgaa ggcaatatgc acgacacggt gatgctgctg gggatgctcg
     3601 gctgggataa atacgacggc aaggtggagt ttatcaccga gctattccca agctctggca
     3661 ccggtcaggt taacgctgtt ttcccgcttc ccgcgtaagg acgttttatg ccgcacttta
     3721 tcgtggaatg tactgaaaat attcgtgaag aagcccgctt acctgagctg tttgccagtg
     3781 ttaacactgc actggcggca acggggattt ttccgcttgg gggaatacgt agccgggcgc
     3841 actggatcga tacctggcaa atggctgatg gcaagcacga ttacgccttt gtgcatatga
     3901 cgctaaaaat cggcagcggg cgcagcctgg aaagccgtca ggaagtgggc gaaatgctgt
     3961 ttgatctgat taaaacgcac ttcgcctcac tgatggagag ccgttacctg gcgctttcat
     4021 ttgagattgc agagctgcac ccgacgctga attttaaaca aaacaacgtg cacgcgttgt
     4081 ttaaataaat ttgcaccagc ttccaggccg gatgataagg cgcagccgca tccggcaatg
     4141 ccacaggata tcgctatgtt cgataaacac acccacaccc tgatcgccca gcgtctggat
     4201 caggcagaaa aacagcgcga acagatccgc gcgatctcgc tggattaccc ggagatcacc
     4261 atcgaagacg cttacgcggt gcagcgtgaa tgggttcgac tgaaaatcgc cgaaggtcgc
     4321 acgctgaaag gccacaaaat cggcctgacc tcgaaagcga tgcaggccag ctcacaaatc
     4381 agcgagccgg attacggtgc cctgctggat gacatgttct tccacgatgg cagcgatatc
     4441 ccgaccgatc gctttatcgt gccgcgcatt gaagtggagc tggctttcgt gctggcaaaa
     4501 ccgctgcgtg ggccaaactg cacgctgttc gacgtctaca acgccacgga ctatgtgatc
     4561 ccggcgctgg agctgatcga cgctcgctgc cacaacatcg atccggaaac ccagcgcccg
     4621 cgcaaagtgt tcgacaccat ttccgataac gccgccaacg ccggggtgat cctcggcggt
     4681 cgtccgatca aaccagacga actggatctg cgttggatct ccgccctgat gtatcgcaat
     4741 ggcgtgattg aagaaaccgg cgtcgccgct ggtgtgctga atcatccggc aaacggcgtg
     4801 gcctggctgg cgaacaaact cgcaccgtat gacgtacaac tggaagccgg gcaaatcatt
     4861 ctcggcggtt cgttcacccg cccggttccg gcgcgtaagg gcgacacctt ccacgtcgat
     4921 tacggcaaca tgggctccat tagctgccgc tttgtttaag gagagaacga tggaaaacag
     4981 ttttaaagcg gcgctgaaag caggccgtcc gcagattgga ttatggctgg ggctgagcag
     5041 cagctacagc gcggagttac tggccggagc aggattcgac tggttgttga tcgacggtga
     5101 gcacgcgccg aataacgtgc aaaccgtgct cacccagcta caggcgattg cgccctaccc
     5161 cagccagccg gtagtacgtc cgtcgtggaa cgatccggtg caaatcaaac aactgctgga
     5221 cgtcggcaca caaaccttac tggtgccgat ggttcaaaac gctgacgaag cccgtgaagc
     5281 ggtacgcgcc acccgttatc cccccgccgg tattcgcggt gtgggcagtg cgctggcccg
     5341 cgcctcgcgc tggaatcgta ttcctgatta cctgcaaaaa gccaacgatc aaatgtgcgt
     5401 gctggtgcag atcgaaacgc gtgaggcaat gaagaactta ccgcagattc tggacgtgga
     5461 aggagtcgac ggcgtgttta tcggcccggc ggatctgagc gccgatatgg gttatgccgg
     5521 taatccacag cacccggaag tacaggccgc cattgagcag gcgatcgtgc agatccgcga
     5581 agcgggcaaa gcaccgggga tcctgatcgc caatgagcaa ctggcaaaac gctatctgga
     5641 actgggcgcg ctgtttgtcg ccgtcggcgt tgacaccacc ctgctcgccc gcgccgctga
     5701 agcgctggca gcacggtttg gtgcgcaggc taccgccgtg aagcccggcg tgtattaatg
     5761 ccggatgcgc tgcgcttatc cggcctacac tcgcaccaaa tcgtaggccg gataaggcgt
     5821 tacgccgcat ccagcaaaaa ccttgtcgta ccctacaaaa atcccattag aggaagaaaa
     5881 atgagcgaca cctcacctgc cataccggag agtatcgatc cggcgaatca gcataaagcg
     5941 ctgactgccg gacaacaggc ggttattaag aagctgtttc gccgcctgat cgtctttctg
     6001 ttcgtgctgt ttatcttctc gttccttgat cgcatcaaca tcggctttgc cggactcacg
     6061 atgggacgcg acctcggtct gagcgccacc atgtttggcc tcgctaccac cctgttctat
     6121 gccgcttatg tcatcttcgg cattcccagc aacattatgc tgagtattgt cggtgcgcgg
     6181 cgctggatcg ccaccatcat ggtgctttgg ggcatcgcct ctactgccac catgtttgcc
     6241 actggcccca ccagcttgta cgtactgcgt atactggttg gcattaccga agccggcttt
     6301 ctgcccggca ttctgctgta tttaaccttc tggtttccgg cctatttccg cgcccgtgcc
     6361 aacgccttgt ttatggtagc aatgccggta acgacagcgt tgggctcgat cgtttccggc
     6421 tacattttgt cgctggatgg cgtcatggca ttaaaaggct ggcagtggct gtttttgctg
     6481 gaaggcttcc cgtcggtatt actcggcgtc atggtgtggt tctggcttga tgactcaccg
     6541 gacaaagcta agtggctgac gaaagaagac aaaaaatgcc tgcaagagat gatggataac
     6601 gatcgtctga cgctgattca gccagaggga gccatcagcc accacgccat gcaacaacgc
     6661 agtatgtggc gggagatctt cactccggtg gtgatgatgt ataccctggc gtatttctgc
     6721 ctgaccaaca cacttagtgc gatcagcatc tggacaccgc agatcctgca aagttttaat
     6781 cagggcagca gtaatatcac catcggcctg ctggccgccg taccgcagat ttgtaccatt
     6841 ctcgggatgg tctactggag ccgtcactca gatcgccgcc aggaacgaag gcatcacacc
     6901 gcccttcctt atttgttcgc tgccgcaggt tggttactgg cttcggcaac tgatcacaac
     6961 atgatccaga tgctggggat cattatggct tcgaccggat cattcagcgc aatggcgatt
     7021 ttctggacaa caccggatca gtccatcagc ctgcgggcac gagcgatcgg tattgcggtg
     7081 atcaacgcca ctggcaacat tggttcagcg ttaagtccgt ttatgatcgg ctggttgaaa
     7141 gatttgaccg gcagctttaa cagtggattg tggtttgttg ccgcgctgct ggtgattggt
     7201 gcggggatta tctgggcgat tccaatgcag tcctcccgtc cgcgagcgac cccgtaagga
     7261 acgacgatgt gtgaccgtca gattgccaat attgatatca gcaaagagta cgatgaaagc
     7321 ctgggcacgg acgatgtgca ttatcagtcc ttcgcccgca tggcggcctt ttttggccgc
     7381 catatgctgc cacatcgcca cgaacagtac tttcagatgc atttcctcaa tagcggacag
     7441 attgagctac aacttgacga tcatcgctac tcggtggaag cgcccctgtt tgtcctgacg
     7501 ccgccgtcag tacctcatgc gtttattacg gaatctgacg ccgacggtca tgtgttgacg
     7561 gtacgggaag atctgatctg gcccctgctg gaagttcttt atccgggcac tagggaaacc
     7621 ttcggcctgc cggggatttg cctgtcactg gcagataaac ccgacgaact ggcggcgctg
     7681 gaacactatt ggcaactgat agagcgggaa tcggtggaac aactgcctgg acgggaacac
     7741 accctgacgt tactggcaca ggcagtgttc accctactgc tgcgtaacgc aaaactcgac
     7801 gaccatgccg ccagcgggat gcgcggagaa ttaaaactgt tccagcgttt taatatgctg
     7861 attgaaagcc attttcatca gcactggaca gtaccggatt acgctaacga actgcatatc
     7921 accgaatcac gactcacgga catctgccgt cgctttgcca accgtccgcc aaaacggttg
     7981 attttcgaca ggcagctgcg agaagccaag cggctgctgc tgttttctga taacgccgtg
     8041 aacaatattg cctggcaact cggttttaag gatccggctt attttgcgcg cttttttaat
     8101 cgcttagtcg gttgctcgcc cagtgcttat cgcgccaaaa aagtacctgt gacgtgaaaa
     8161 tcccttagcc ttaacgggaa accaggcacc acctgctatt cccctttctc cgcctggagc
     8221 aaggggattt agtaacgtca aaaatcgcta aaagcgacct cgatcacaaa accgtacccg
     8281 acctgaaaag taccagccat tgtcccaaaa gtctcttccc gtaatcgccc ctgcaagctt
     8341 caattgaata acaaaaacaa cacacaaaat taacaatcgt attccgatta atactgtaga
     8401 ggtcgacatg aaaccagaag atttccgcgc cagtacccaa cgtccgttca ccggggaaga
     8461 gtatctgaaa agcctgcagg atggtcgcga gatctatatc tatggcgagc gagtgaaaga
     8521 cgtcactact catccggcat ttcgtaatgc ggcagcgtct gttgcccaac tgtacgacgc
     8581 gctacacaaa ccggagatgc aggactctct gtgctggaac accgacaccg gcagcggcgg
     8641 ctatacccat aaattcttcc gcgtggcgaa aagtgccgac gacctgcgcc agcaacgcga
     8701 tgccatcgct gagtggtcac gcctgagcta tggctggatg ggccgtaccc cagactacaa
     8761 agctgctttc ggttgcgcac tgggcgcgaa tccgggcttt tacggtcagt tcgagcagaa
     8821 cgcccgtaac tggtacaccc gtattcagga aactggcctc tactttaacc acgcgattgt
     8881 taacccaccg atcgatcgtc atttgccgac cgataaagtg aaagacgttt acatcaagct
     8941 ggaaaaagag actgacgccg ggattatcgt tagcggtgcg aaagtggttg ccaccaactc
     9001 ggcgctgact cactacaaca tgattggctt cggctcggca caagtgatgg gcgaaaaccc
     9061 ggacttcgca ctgatgttcg ttgcgccaat ggatgccgat ggcgtgaaat taatctcccg
     9121 cgcctcttat gagatggtcg cgggtgctac cggctcgcca tacgactacc cgctctccag
     9181 ccgcttcgat gagaacgatg cgattctggt gatggataac gtgctgatcc catgggaaaa
     9241 cgtactgatc taccgcgatt tcgatcgctg ccgtcgctgg acaatggaag gcggttttgc
     9301 ccgtatgtat ccgctgcaag cctgtgtgcg cctggcagtg aaattagact tcattacagc
     9361 actgctgaaa aaatctctcg aatgtaccgg caccctggag ttccgtggtg tacaggccga
     9421 tctcggtgaa gtggtggcgt ggcgcaacac cttctgggca ttgagtgact cgatgtgttc
     9481 tgaagcgacg ccgtgggtca acggggctta tttaccggat catgccgcac tgcaaaccta
     9541 tcgcgtactg gcaccaatgg cctacgcgaa gatcaaaaac attatcgaac gcaacgttac
     9601 cagtggcctg atctatctcc cttccagtgc ccgtgacctg aacaatccgc agatcgacca
     9661 gtatctggcg aagtatgtgc gcggttcgaa tggtatggat cacgtccagc gcatcaagat
     9721 cctcaaactg atgtgggacg ccattggcag cgagtttggt ggtcgccacg aactgtatga
     9781 aatcaactac tccggtagcc aggatgagat tcgcctacag tgtctgcgcc aggcacaaag
     9841 ctccggcaat atggacaaga tgatggcgat ggttgatcgc tgcctgtcgg aatacgacca
     9901 gaacggctgg actgtgccgc acttgcacaa caacgacgat atcaacatgc tggataagct
     9961 gctgaaataa cgcagcagga ggttaagatg caattagatg aacagcgcct gcgctttcgt
    10021 gacgcgatgg ccagcctgtc ggcagcggta aatattatca ccaccgaggg cgacgccgga
    10081 caatgcggga ttacggcaac ggccgtctgc tcggtcacgg atacaccacc atcgctgatg
    10141 gtgtgcatta acgccaacag tgcgatgaac ccggtttttc agggcaacgg caagttgtgc
    10201 gtcaacgtcc tcaaccatga gcaggaactg atggcacgcc acttcgcggg catgacgggc
    10261 atggcgatgg aagagcgttt tagcctctca tgctggcaaa aaggtccgct ggcgcagccg
    10321 gtgctaaaag gttcgctggc cagtcttgaa ggtgagatcc gcgatgtgca ggcaattggc
    10381 acacatctgg tgtatctggt ggagattaaa aacatcatcc tcagtgcaga aggtcacgga
    10441 cttatctact ttaaacgccg tttccatccg gtgatgctgg aaatggaagc tgcgatttaa
    10501 gctccagccc cttcacgcat tcgtcgaagg ggcattttta ttgtgatctc aatcacatat
    10561 ccacccgatg aataattctt gcccaaccgc tcaccgccat tcctgaccgc ttacatccct
    10621 aaaataacca ctcagttatt taccttactt tacgcgcgcg taactctggc aacatcactg
    10681 caggatagcg gtcaatttac ctcctcaaac gcaacgcaaa cctagaacgg cttcggccta
    10741 tgattaacaa attactctat gaattttggt ttgcaggaag gcagcaagtg agtgaatccc
    10801 cgggagcttg caacagtaag tgacaggggt gaacgaacgc agctgccgca cctgtaagcc
    10861 aaaagacgac gagtaaaatg ccaggtttta ctatggatac gaaaaaacta ttcaagcaca
    10921 taccctgggt gattctcgga atcatcggtg cattctgcct cgcggtagtt gcattacgtc
    10981 ggggggagca cgtcagcgcc ctgtggatcg tggtcgcctc tgtgtcggtg tatctggtgg
    11041 cgtatcgcta ctacagtctg tacatcgccc agaaggtgat gaaactcgac cccacgcgcg
    11101 cgacgcctgc ggttattaac aacgacggtc tgaactacgt tccgaccaac cgttacgtgt
    11161 tgtttggtca ccacttcgcc gctatcgccg gtgctggtcc gctggttggt ccggttctcg
    11221 ccgcgcagat gggctaccta cctggcacgc tgtggctgct ggcgggggta gtactggccg
    11281 gtgcggtaca ggactttatg gtgctgttta tctcctctcg ccgtaacggc gcatctctcg
    11341 gtgagatgat caaagaagag atgggaccag taccggggac tatcgcgctg tttggctgtt
    11401 tcttaatcat gatcatcatc cttgccgtcc tggcgctgat tgtggttaaa gccctggccg
    11461 aaagcccgtg gggtgttttc accgtttgct caaccgtacc gattgcgctg tttatgggta
    11521 tctacatgcg ctttatccgt ccggggcgtg tgggtgaagt ctctgtcatt ggtatcgtgc
    11581 tgctggttgc ctctatctac ttcggtggcg tgattgctca cgatccgtac tggggtccgg
    11641 cactgacctt taaagacacc accattacct tcgcgctgat tggctatgcg tttgtttccg
    11701 cactgctgcc ggtgtggctg atcctcgcac cgcgcgacta tctggcaacc ttcctgaaaa
    11761 tcggcgttat cgtcggcctg gcgctgggta ttgtggtgct gaacccggaa ctgaaaatgc
    11821 ctgcgatgac ccagtacatt gacggtactg gcccgctgtg gaaaggcgct ctgttcccgt
    11881 tcctgttcat caccatcgcc tgtggtgcgg tatctggctt ccacgcgctg atctcttccg
    11941 gtacgacgcc gaaactgctg gctaacgaaa ccgacgcgcg tttcattggc tacggcgcaa
    12001 tgctgatgga gtccttcgtg gcgattatgg cgctggttgc tgcgtccatc atcgaaccgg
    12061 gtctttactt cgcgatgaac accccgcctg ctggccttgg cattaccatg cctaacctgc
    12121 atgaaatggg tggcgagaat gcgccgatca tcatggcgca gctgaaagac gttaccgcac
    12181 acgcggcagc gaccgtcagc tcctggggct tcgtgatttc gccagagcag atcctgcaaa
    12241 ccgcgaaaga cattggtgag ccttctgtcc tgaaccgtgc aggtggtgcg ccaacgctgg
    12301 cggtaggtat cgctcacgtg ttccacaaag tgctgccgat ggctgacatg ggcttctggt
    12361 atcacttcgg tattctgttc gaagccctgt tcatcctgac cgcgctggat gcgggtactc
    12421 gttctggccg ctttatgctg caagacctgc tgggtaactt catcccgttc ctgaaaaaaa
    12481 ccgactctct ggttgccggt atcatcggta ctgcgggctg tgtgggtctg tggggctacc
    12541 tgctgtatca gggcgtggtc gatccgctgg gcggcgttaa gagcctgtgg ccgctgttcg
    12601 gtatttccaa ccagatgctg gcagccgtag cgctggtact gggcaccgtt gtgctgatta
    12661 agatgaagcg cacccaatac atctgggtaa ctgttgttcc ggctgtatgg ctgcttatct
    12721 gcaccacctg ggcgctgggt ctgaaactgt tcagcaccaa cccgcagatg gaaggcttct
    12781 tctacatggc aagccagtac aaagagaaga ttgctaacgg tactgacctg acggcgcagc
    12841 agattgccaa catgaaccac atcgttgtga acaactacac caacgcaggc ctgagtattc
    12901 tgttcctgat tgtggtgtac agcatcatct tctacggttt caaaacctgg cttgcggtgc
    12961 gtaacagcga caaacgtact gacaaagaaa ctccgtacgt tccaatcccg gaaggcggcg
    13021 tgaagatctc ttcgcaccac taaccgtgtt tagccccgct tcggcggggc tttgttctta
    13081 tcagagtgaa ctatgtttgg taacttagga caggcaaaaa aatatctcgg ccaggcggcg
    13141 aagatgttga ttggcattcc agactatgac aactacgtcg agcatatgaa gaccaaccat
    13201 cccgacaagc cgtacatgag ctatgaagaa ttcttccgcg aacgccagaa cgcacgctac
    13261 ggcggcgatg gtaaaggcgg tatgcgctgt tgttaatgga gagagcatga acccgattgc
    13321 agttacccta ctcaccggtt ttttaggcgc gggcaaaacc accctgctgc gccatattct
    13381 taatgaacaa cacggctaca agattgccgt gattgaaaac gaattcggcg aagtctccgt
    13441 tgatgatcaa ttgattggtg atcgcgcaac gcagatcaaa acgctgacca acggctgcat
    13501 ctgctgttcg cgctccaacg agctggagga cgcgctactc gacctgctgg ataatctcga
    13561 caagggcaat attcagttcg atcgtctggt cattgaatgc accggcatgg ccgatcctgg
    13621 cccgattatt caaacctttt tctcccatga gattttatgc cagcgctacc tgctggacgg
    13681 cgtgattgcg ctggtggatg cggtacatgc cgatgagcag atgaaccagt tcaccatcgc
    13741 ccagtcccag gtgggctacg ccgaccgtat tctgctgact aaaaccgacg tcgcaggcga
    13801 agcagaaaaa ctgcgtgaac gcctggcgcg catcaacgcc cgtgcaccgg tctacaccgt
    13861 cacccacggc gacatcgacc tgggcctgct gttcaacacc aacggtttta tgctggaaga
    13921 aaacgtcgtc agcaccaaac cgcgtttcca ctttatcgcg gataaacaaa acgatatttc
    13981 gtcgattgtg gtggaactgg attacccggt agatatcagc gaagtttccc gggtgatgga
    14041 aaacctgctg ctggaatcgg cggataaact gctgcgttac aaagggatgc tgtggattga
    14101 cggcgaacct aaccgcctgc tgttccaggg cgtccagcgc ctctacagcg ccgactggga
    14161 ccgcccgtgg ggcgatgaaa caccgcatag cacgatggtg tttatcggga ttcagttgcc
    14221 ggaggatgag attcgggcgg cgtttgcggg gttgaggaag taaggggtta tgggccggat
    14281 aaggcgcagc cgcatccggc ctgatatttc actcaaaata gtccatatcc agtttcggca
    14341 ccttcaacaa acgtgaagaa acccctactt cgttttcgat cattaagtgc accaggcgtt
    14401 ccccatcaac caacaccata ccctcgacgg attgagcaaa gtcacgcgcc tgagacgtaa
    14461 atccagaagt ggtaataaac accccacgtt tcgccttttg cccagccagt gcgccgtaaa
    14521 atgcctgtaa ttctggcctg cctacagtgt tctgccaacg ttttgcctga acataaactt
    14581 tctccaggcc aagtttatca agcgatatca caccatcgat gccaccatct ccagtaccgc
    14641 caacacgctg caaatcatca cggtggccgc cataccccag gcgatgcaaa acatccagaa
    14701 caatgacttc aaagcgcgaa ggagaaacct gcaataagtt ttccagaacc tcatcagcca
    14761 ccgcatcacg aagctctttt agcgcctgat ctaaccgatc gtccgggctg ctctttgcta
    14821 gttcttcatg atcgggagag tcggctttcg gatctaaatc gacggcatcc ggccgtgact
    14881 taagtttgac attcacaaaa tcgaaggcca gatggttcgt ctcctgctcc gtcattggct
    14941 ggggatgaga cgcaacccag tcaaaacccg caggagtcag gcaccatttg ccacgcgaca
    15001 aactttgcga caacccggca cgttttaaac ggtcatgtgc ccagcctgca cgatttttat
    15061 aaacaagttg tccgctggta atgactttcg ctcgctggct gtcatccagt cctaatgcat
    15121 ccgcggcagc ctcatgaaca tcacgcgcgg ctgcaccttc cggttttgtc gccagataac
    15181 gcagaagagg ttcaatatat ttgtcatagg taggaaccgt catagtacat ccttgcagaa
    15241 tcaggtagat gtttttcggc tactataaca ctaccaaaat agacgaacac gttagaaatg
    15301 agtcagttgc tgtgactccg gtcattgtcc ggaaaggtac agaaagctaa gataagacgt
    15361 tatgggcctt aaatatttgg acaggcccac acagcaatgg attaataaca atgatgaata
    15421 aatccaactt cgaattcctg aagggcgtca acgacttcac ttatgccatc gcctgtgcgg
    15481 cggaaaataa ctacccggat gatcccaaca cgacgctaat taaaatgcgt atgtttggcg
    15541 aagccacagc gaaacatctt ggtctgttac tcaacatccc cccttgtgag aatcaacacg
    15601 atctcctgcg cgaactcggc aaaatcgcct tcgttgatga caacattctc tctgtatttc
    15661 acaaattacg ccgcattggt aaccaggcgg tgcacgaata tcacaacgat ctcgacgatg
    15721 cccagatgtg cctgcgactc gggttccgcc tggctgtctg gtactaccgt ctggtcacta
    15781 aagattatga cttcccggtg ccggtgtttg tgttaccaga acgtggcgag aacctctatc
    15841 accaggaagt gctgacgcta aaacaacagc ttgaacagca ggtgcgagaa aaagcgcaga
    15901 ctcaggcaga agtcgaagcg caacagcaga agctggttgc cctgaacggc tatatcgcta
    15961 ttctggaagg caaacagcag gaaacagaag cgcaaaccca ggctcgcctt gcggcgctgg
    16021 aagcacagct cgccgagaag aacgcggaac tggccaaaca gactgaacag gaacgtaagg
    16081 cttaccacaa agaaattacc gatcaggcca tcaagcgcac actcaacctt agcgaagaag
    16141 agagccgctt cctgattgat gcgcaactgc gtaaagcagg ctggcaggcc gatagcaaaa
    16201 ccctgcgctt ctccaaaggc gcgcgcccgg aacccggcgt caataaagcc attgccgaat
    16261 ggccgaccgg gaaagatgaa acgggtaaac agggctttgc ggattatgtg ctgtttgtcg
    16321 gcctcaaacc catcgcggtg gtagaggcga aacgtaaaaa tatcgacgtt cccggcaagc
    16381 tcaatgagtc gtatcgctac agtaaatgct tcgataatgg cttcctgcgg gaaaccttgc
    16441 ttgagcacta ctcaccggat gaagtgcatg aagcggtgcc ggagtatgaa actagctggc
    16501 aggactccag cggccaacaa cggtttaaaa tccccttctg ctactccacc aacgggcgcg
    16561 aataccgcgc agcaatgaag actaaaagtg gcatctggta tcgcgacgtg cgtgataccc
    16621 gcaatatgtc gaaagcctta cccgagtggc atcgcccgga agagctgctg gaaatgctcg
    16681 gcagcgaacc gcaaaaacag aatcagtggt ttgccgataa tcccggcatg agcgagctgg
    16741 gcctgcgtta ttatcaggaa gatgccgtcc gcgcggtgga aaaggcaatc gtcaagggtc
    16801 aacaagagat cctgctggcg atggcgaccg gtaccggtaa aacccgcacc gcaattgcca
    16861 tgatgttccg cctgatccag tcccagcgtt ttaaacgcat tctcttcctt gtggatcgcc
    16921 gttctcttgg cgagcaggcg ctgggggcgt ttgaagatac gcgtattaac ggcgacacct
    16981 tcaacagcat tttcgacatt aaagggctga cggataaatt cccggaagac agcaccaaaa
    17041 ttcacgttgc caccgtacag tcgctggtga aacgcaccct gcaatcagat gaaccgatgc
    17101 cggtggcccg ttacgactgt atcgtcgtcg atgaagcgca tcgcggctat attctcgata
    17161 aagagcagac cgaaggcgaa ctacagttcc gcagccagct ggattatgtc tctgcctacc
    17221 gtcgcattct cgatcacttc gatgcgataa aaatcgccct caccgccacc ccggcgttgc
    17281 atacggtgca gattttcggc gagccggttt accgttatac ctaccgtacc gcggttatcg
    17341 acggttttct gatcgaccag gatccaccaa ttcagatcac cacccgcaac gcacaggagg
    17401 gtgtttatct ctccaaaggc gaacaggtag agcgcatcag cccgcagggg gaagtaatca
    17461 acgacaccct ggaagacgat caggattttg aggtcgccga ctttaaccgt ggcctggtga
    17521 tcccggcgtt taaccgcgcc gtctgtaacg aactcaccaa ctaccttgac ccgaccgggt
    17581 cgcaaaaaac gctggtcttc tgcgtcacca atgcccatgc cgatatggtg gtggaagagt
    17641 tgcgtaccgc gttcaagaaa aagtatccgc aactggagca cgacgcgata atcaagatca
    17701 ccggtgatgc cgataaagac gcgcgcaaag tgcagaccat gatcacccgc ttcaataaag
    17761 agcggctgcc caatattgtg gtaaccgtcg acctgctgac gaccggcgtc gatattccgt
    17821 cgatctgtaa catcgtgttc ctgcgtaaag tacgcagccg tattctgtac gagcagatga
    17881 aaggccgcgc cacgcgcttg tgcccggacg tgaataaaac cagctttaag attttcgact
    17941 gcgtcgatat atacagcacg ctggagagcg tcgacaccat gcgtccggtg gtggtgcgtc
    18001 ctaaggtgga gctgcaaacg ctggtcaacg aaattaccga ttcagaaacc tataaaatca
    18061 ccgaagcgga tggccgcagt tttgccgagc acagccatga acaactggtg gcgaagctcc
    18121 agcgcatcat cggcctggcc acgtttaacc gtgaccgcag cgaaacgata gataaacagg
    18181 tgcgtcgtct ggatgagcta tgccaggacg cggcgggggt gggctttaac ggcttcgcct
    18241 cgcgcctgcg ggaaaaaggg ccgcactgga gcgccgaagt ctttaacaag ctgcctggct
    18301 ttatcgcccg cctggaaaag ctgaaaactg atatcaataa cctgaatgat gcgccgatct
    18361 tcctcgatat cgacgatgaa gtgttgagcg taaaatcgct gtacggtgat tacgacacgc
    18421 cgcaggattt cctcgaagcc tttgactcgc tggtgcaacg ttccccgaac gcgcaaccgg
    18481 cattgcaggc ggtgatcaat cgcccgcgcg atctcacccg taaagggctg gtcgaactcc
    18541 aggagtggtt tgaccgccag cactttgagg aatcttccct gcgcaaagcg tggaaagaga
    18601 cgcgtaatga agatattgcc gcccggttga ttggacatat tcgccgcgct gcggtgggcg
    18661 atgcgctgaa accgtttgag gaacgtgtcg atcacgcgct gacgcgcatc aagggcgaaa
    18721 acgactggag cagcgagcaa ttaagctggc tcgatcgttt agcacaggcg ctgaaagaga
    18781 aagtggtgct cgacgacgat gtcttcaaaa ccggcaactt ccaccgtcgc ggcgggaagg
    18841 cgatgctgca aagaaccttt gacgataatc tcgacaacct gctcgataaa ttcagcgatt
    18901 atatctggga cgagctggcc tgacacgtat acacttcatc cttcaggctg cctctgcgtt
    18961 ggctgcgctc gttcaccccg gtcacgtact tctgtacgct cccggggatt cactcacttg
    19021 ccgccttgat gcaacctgaa tgattttgtg tatattaccc tcggcaattt attcttatgc
    19081 ggcccaataa atttgggccg ctgcttaatt tacggaactc acaatgaaca ataacgatct
    19141 ggtcgccaag ctgtggaagc tgtgcgacaa cctgcgcgat ggcggcgttt cctatcaaaa
    19201 ctacgtcaat gaactcgcct cgctgctgtt tttgaaaatg tgtaaagaaa ccagacagga
    19261 agcggaatac ctgccggaag gctaccgctg ggacgacctg aaatcccgta tcggtcagga
    19321 gcagttgcag ttctaccgta acctgctggt acatctgggc gccgacaatc aaaagctggt
    19381 gcaggcggtg ttccagaacg tcaacaccac cattacccag ccaaaacagt tgaccgaact
    19441 ggtcagcaat atggattcac tggactggta caacggtgcg cacggtaagt cgcgcgatga
    19501 cttcggcgat atgtacgaag ggctgttgca gaagaacgcg aacgaaacca agtctggcgc
    19561 gggccagtac ttcaccccgc gtccgctgat taaaaccatt attcatctgc tgaaaccgca
    19621 gccgcgtgaa gtggtgcagg acccggcagc gggtacggcg ggctttttga ttgaagccga
    19681 ccgctacgtt aagtcgcaga ctaacgatct ggacgacctt gatggcgaca cacaggattt
    19741 ccagatccac cgcgcgttta tcggcctcga actggtgccc ggcacccgtc gtctggcgct
    19801 gatgaactgc ctgctgcacg atattgaagg caacctcgac cacggcggcg caatccgtct
    19861 gggcaacacc ctgggcagcg acggtgaaaa cctgccgaag gcgcatattg tcgccactaa
    19921 cccgccgttt ggcagcgccg caggcaccaa cattacccgc acctttgttc acccgaccag
    19981 caacaaacag ctgtgcttta tgcagcatat tatcgaaacg ctgcatcccg gcggtcgtgc
    20041 ggcggtggtg gtgccagata acgtgttgtt tgaaggcggc aaaggcaccg acatccgtcg
    20101 tgacctgatg gataagtgtc atctgcacac cattctgcgt ctgccgaccg gtatttttta
    20161 cgcgcagggc gtgaagacca acgtgctgtt ctttaccaaa ggcacagtgg cgaacccgca
    20221 tcaggataag aactgcaccg acgacgtgtg ggtgtacgac ctgcggacca atatgccgag
    20281 ctttggtaaa cgcacgccgt ttaccgacga gcatttgcag ccgtttgagc gcgtgtatgg
    20341 cgaagatccc cacggcttaa gcccgcgtac tgaaggggaa tggagtttta acgccgaaga
    20401 gacggaagtt gccgacagcg aagagaacaa aaataccgat cagcatctgg ctaccagccg
    20461 ctggcgcaag ttcagccgcg agtggatccg ctccgcgaaa tccgattcgc tggatatctc
    20521 ctggctgaaa gataaagaca gcattgacgc cgacaacctg ccggagccgg atgtattagc
    20581 ggcagaagcg atgggcgaac tggtacaggc gctgggtgaa ctggatgcgc tgatgcgtga
    20641 actgggggcg agcgatgagg cggatgcaca gcgtcagttg ctggaagaag cgtttggtgg
    20701 ggtgaaggaa tgagtaaaaa ttggcaaata tgccagttgg gtgatgtttt gaccctcatc
    20761 aatggcagag catataagaa agaagaaatg ctcacagaag gtgttcctat tctcagaatt
    20821 caaaatttga atggagggaa taaatggttt tattccaact tagagctaca ggaagataaa
    20881 tattgctata aaaatgatct cctatatgct tggtcagcaa catttggtcc atattggtcg
    20941 aagtgggatc ataaactaat ttatcactac catatttgga agattgaaac atcccctgca
    21001 cttaataagc aatttgctta ttatgcatta ttgaatatta cagaggcact taaagcatcg
    21061 gctcacggcg tggcaatgcc acatattacc aaagctggaa tggaggcttg ggaaatacaa
    21121 cttcctccaa tttcagaaca aaaaatcatc gctgaaaaac tcgatacgct gctggcgcag
    21181 gtagagagca ccaaagcacg tcttgagcaa atcccacaaa tcctgaaacg ttttcgtcag
    21241 gcggtgctgg gtgccgcggt aagtggaaaa ctaactgaag actggagaga taattcgagc
    21301 ctgtctgggt ggatagaagg caaacttggg gaatttataa aaaaaccaag ttatggaacg
    21361 tcatctaagt ctaataaaga aggactcatt cctgtattaa gaatgggcaa tcttcagggc
    21421 ggaaaattgg actggacgga tctagtctat acttcagata ccatagaaat tgaaaagtat
    21481 aaacttgagt acaatgatgt attattcaat cgtacaaata gtcctgaact tgtcgggaaa
    21541 acagccatat ataaatcaga gcaaccagct atttatgcag gttatttgat tagggttcaa
    21601 tgtttacctg atttaaaccc tgactatctt aactaccatt taaatagcat tttggggagg
    21661 caatattgct actcagtaaa atctgatggg gtcagtcaat caaatattaa tgcacagaaa
    21721 ttaattgcat acccaataac tgttcctcca ctccccgaac aacacgaaat cgttcgccgc
    21781 gtcgagcagc tcttcgccta cgcagacacc atagaaaaac aggtcaacaa cgccttagcc
    21841 cgcgttaaca acctgacgca atccatcctg gcaaaagcgt tccgtggtga actcaccgcc
    21901 cagtggcggg ccgaaaaccc ggaattaatc agcggagaaa acagcgccgc cgcgttgctg
    21961 gaaaaaatca aagctgaacg cgcagccagc ggaggtaaaa aagcctcgcg taaaaaatcc
    22021 tgatcattat tttctggcgc acctttccgg tgcgcctttt attatttcac gccaatcata
    22081 actcacataa atatatttaa atcattccag gaactgacca ttttattcta tttttagctg
    22141 gactttccct gtatttactg atgatatatc caggtattta gcgcggtgcg gatgtgcgcc
    22201 aacacacccg caccgctcat cacaatcact attatcggag aatagcagtt atgactgaca
    22261 cgtattctat tgcacaaccg ttcgaagcag aagtctctcc ggcaaataac cgtcatgtca
    22321 ccgtcggtta tgcgagccgc tacccggatc acgcgcatat tcccgccatc accctgaaag
    22381 gtcagtggct ggaagccgcc ggttttgcca ccggcacggc ggtagatgtc aaagtgatgg
    22441 aaggctgcat cgtcctcacc gctcaaccac ccgccgccga agagagcgaa ctgatgcagt
    22501 cgctgcgcag ggtgtgcaag ctgtcggcgc gtaaacaaaa gcaggtgcag gaatttattg
    22561 gggtgattgc cggtaaacag aaagttgcct gaatggaaaa acgctcccga caccacatcg
    22621 ggagcggcac atcatcagga ggcgctcacc tccaccagca catccgttgc ccagctctct
    22681 tcgctgttgg ccacatttcc ttcattgcag gcctgagcca ttttgactaa ctggaaagaa
    22741 acgtcatcgc ggaagtcaaa gtaatgccat tgcgatgcaa agttgatacg ccagtagccc
    22801 atcccctgat cgaagcggat cttcaaaacc tgaccatcct caaaagtcac cgtcagttta
    22861 cgatggtgag ggatatcgcg cggcgagtcg aacaccgtta gttctatcgg cctgcccagc
    22921 gcagcagcaa accactgatc agcgaaatcc tgaaaatccg ctatgctcat ccagtcatgg
    22981 aaaggccggt taccggggcg atctttatct ttaaacagcg tatccagcgc cacttcagcg
    23041 acatcggtca gctttgtttt tagcggtctc agaattgatc ccaacaacgc cagcgcaacc
    23101 gggttttgca gatagcgatc ggtatagtga acgccggtaa ttcgcgtgtt attcatcagt
    23161 gactgtgctt cttcatggtc gttaaacagc acgtcccaga agcgttgtcc aaactgcgaa
    23221 agtggtccat tcaactgttt gtggatctga atgtctttga cccgctcgac agcgttggcg
    23281 gctttagccg gcagaataaa ctcgttcagt tctaccgttt tataaccgag actacgcacc
    23341 accagttcgt cattcaggtg ccactccggc cccggaatcg ttgcctgctg gctgcgagaa
    23401 gccagcgtca tcacgcgatc ggcaaaggta acctgggcaa cgatcggcaa ttgcagatcc
    23461 tgttcaacat ggcacagccg cacacccagc gcagtaaatt gcgatagttc atacaacaga
    23521 tccgggtcat ccactcccgc aggtataaca agatcgacct cgacgttgtc ttttagccga
    23581 tagctctgaa ccgccgcgcg gaactgacgg gcgtataaat cccactcatt gggcgcgccg
    23641 gtcatccaca gggtgagctt ttcagcgcct tcgttaatcg cccggcgaat cgtatcgcca
    23701 atagcgccag ggcagtagcg ggcatccggc agcgagaatg tctcttcatc cggtaaaccg
    23761 atgtaatacg tgaagtcatc gccaagccag gccagcgccg ctttgcgatc cagcaaatcg
    23821 tgatcgtggc gagtctggga atcaagcagg cattcactac atgcagtatc acaatggcgg
    23881 caacctaact gtttcaccat tccctgcaaa atagcttcaa tgtgtaccgg tgcgctggag
    23941 gcaaaacctg caccaccgct gatcacatca taaagctgaa cggctaatac cgattgccca
    24001 tcctccagtc tgaccgggcg tacagaatag ccgagttctg cgccggagat acccagaacg
    24061 cccgctagcg cctgacgcag agcaaccgcc agagtcatag cgacaatccg cccctcttct
    24121 gtgttatctg gcagatactc gcctttttgc ggtttgcgca gaaccatttc aaagacatcg
    24181 gtgcgcgcca gtgcgcccag ggtgacgttc ttcatgagtg ccgtcgaacc tgggcaaatc
    24241 agacgatgat tctggctgtc gcggtccgct ttacccggac gcggtggata gtgagcttcc
    24301 atacttttcg gagcatcatt ttcattcagc atcgactccg cacggccaca gctcagacac
    24361 aatgcataac cgtgtccacc ttcccccaga ctttgctgga aaacatgccc atcagcccca
    24421 gaagccatat aacccattag cgggttaggc aacggtacgg gctctgcttt aacaaagacc
    24481 caggcgggga caaccgggac gaatttcatg gtttcaatat tgttcgttac cggagcgtgt
    24541 gcatcggtga caaacccggc aggctgcaac acctgacggc gattatccat gattattttt
    24601 tcgccgcagg cgctattgga gcaaaagagc atcccggaac tgctcatccc ctcttcatag
    24661 ccaatggtcc cgcatttgtg acagcgccag gcacaatcca gacgctgggc ttcattggta
    24721 tcagcattaa tattgtgcca gtgcagtgag acaccggcag agcggaagac acggccatca
    24781 agaatgatct ctgctcccgg tgcatattca cgaatggcaa caccgaggtt acgggaaggt
    24841 aatcctttat aacgggagac gttatcttca cggtcctttt tatcacgact tttttgcaat
    24901 ttttcgcgga tataatcttc cattgtgaag ttatcaaacg tcactacatc tgtcgggaag
    24961 ccgtatcccg gcaggaaagt tctggcagcc agatcgcgca gcagatactc tccacagtgt
    25021 cgtttcttct ccagctctat gcgcttacga taaggcgttt gtggctgcga ctcacgctcc
    25081 tgggtcacga gatcgcgata aacggagagc caccgctgtt gcaggaaggt gatcgcgctg
    25141 attgttttat cccgcagatg ttcagctctt gcaccatgca acgccgtacc tttcaccagc
    25201 cgttccagcg ccgtgtcgat atcaagaacc gggcgttcca gccagatttt aaagcgttcg
    25261 cattttgact gcccgtcatc ttcaccgaaa aaccagaggc tatcgaggct ggttttctct
    25321 ttatcggttt cgccaataac gttacataaa aagtcggaca gtaataatgc attgacgtga
    25381 cgttgtacca gtcgggcgga gttcatcgcg accatgggtg ccgggatcat ggtttcaaac
    25441 ggccagagcg ggttggcaaa aacctgttgg tcgtgtgggt tacctttgca tagggtgtaa
    25501 gagatcgcac gcgactcttt actacgcccg gctcgcccgg cgcgctgtaa atagttggca
    25561 ggatgtggcg gtacattgtt cattaccacc gctgtaatgc ccccgatatc aacccccatt
    25621 tccatggtgg tggaacagtt gagaacattt agttgcccgt tcttgaacat cttttcatag
    25681 ctttgcaggc gttctgacga ctgctgggca gagtgctcgg cagtgcgata gtagaaaccg
    25741 ccttccacga cacgatcgtt gatgtctgtc cagacgtttt ctgaccgcaa ttgtgcgatc
    25801 agcggatcat tgctgaccca gtcgcgtgct tttgccagac cctctgcgta gtcttcctgt
    25861 gaacgatcca gcttccagat ttccggcatg gtgactttct gcgcgacaaa agtgtcatat
    25921 tgcgcctggg taagatgctc gaacgaaata tgggtaggca gataaggggt taagccttta
    25981 aaagccgtat ccaggatttt attggttacc gggcaaatcc aggcatccgt gattaaagaa
    26041 aaagtcatat gctctttcgg taaataaaaa cggttgccat cgggtttcag tactgcaagc
    26101 ggtccggtaa gttgggccca tgcttctttc agccatgtat tgataatatc aatagtcgcc
    26161 gtattgacgg ttttgaatcc agcccccagc atcagtaact tcgctaaacg atgggatacg
    26221 ttgccattac gaatttgagg ccagcgtcta ttctgattat cttcaggatc ttttgattcc
    26281 gggttacgga cgaattttga tgaaaaacgg ctaccaatcc agtttttcag ctcatcatcc
    26341 agctgtgtga agttgctctc gcgaacgtaa tgatccaacg taaccttgag aaaatcgcgc
    26401 cagtcatcca gcgttaattc cctttcctgc cagtgcatag ggagcttatg tattttctcc
    26461 agtcccaggt aaccaacctg aaccagcccc tgtgtttcca ggctgttagt ccgtttaggg
    26521 cgacgcatga attcccggaa caacaacatt tcagaaagct ttagaggccc gccgttttca
    26581 ttaaacactt caggcttcaa atagtagtta tatttcagaa ccgggccacg gatatcggct
    26641 ttatctttaa gctcgttaac catttcagtc caggtgcggg atactagggt ggttgctgct
    26701 tttccgccga ttgcaaactg ataagcctgt tcaagctgtt cagcctgcgc ttgtgacaat
    26761 ttcgcctggt ctggcattcc ccagcttcga tattcttctg cctgctcacg ggcttgcttc
    26821 gccctggtcg ctaatttttc cagatcggca ttggcattcg gcgctgtaga cgtttgcgtc
    26881 ctttgatgcc agctgagaat ttcgactacg cttccgcgta agcgactgcg ttctgcttct
    26941 tgctgcatac gcaccgccat tcgggctgtc ccttgtcgac tgtctgtaaa ggtgatgagt
    27001 cgacgacctc gtcctggcaa cgaatctggt ccgacgccct ctttgccttc atcactggta
    27061 aagtcctgac aatactctag aacggtgggc acgatattag taacgtaaaa tggcccacct
    27121 aatagtgcac gccggaaggg ctgtttccca ctcatgcctc tgtagccaca gccactggca
    27181 ctacaaacct gttcaacatc attaataatc agcggaatgc tgtcattgtt aataacgcca
    27241 atacggcgcg tctggcggtc gaggcgttgt agggtataac cggcctcgct ggtctctcct
    27301 gcggcaataa tcaacggagg ctggaaactg ttctcttttt cgactttttc cgctgtagcg
    27361 tcatgttcaa caggtacttc atcctgcaaa gagaattcat cgccaccttt attttcccac
    27421 tggactagtt tgcccttttt gtcccgcgcc agaagatgag gttcattaca atcattacag
    27481 aacgccactt cgtaaacagg gcttccacaa tcgcaatttt gtcgctggtt cacatataca
    27541 tagccaaagg gccagccttt ttcgagcggt gtaccgtgct ttaacctgca atctttatcc
    27601 acacaagccc atattccctg ggtattacgc tggaagatat gcgccctgac tttaagaaat
    27661 gcgggatcct ttgcattagg ttgggtccct gaacagacgt cgatccaacg cagtacttca
    27721 tgctggctgt gatgctgttt tgtcagtatg tttaaccgct ctgtcatctc atccagcttc
    27781 attggattgg gatgtttgac caacatctcg cgtatgtaac gtgcttcggg ggagtgcgtg
    27841 agcgcataaa agcgctcagg acttatccct ttaattttat tatcattttt atcttttaga
    27901 tccagatcag gtatttgctc tatttcgtct aacggaacag aaacgttttt ggatggttcc
    27961 agttcaggaa tcacccgact tccgtccaga acatcgatgc gctcctgcgg gataccggat
    28021 aattcagaga gaaatttttt cagctgcttt tcggcgtcgc tgccggcaat ggtcgcagag
    28081 gtcgctacaa agcgcacatc atctggcgtc acgccaaacg ctgtcatcac acgacgcagc
    28141 tggagtgcca gttcagcggc ctgcgacccg acataggtat gcgcttcatc cagtacaatc
    28201 cagcgtagtg atttttgtgc tttggattgc tggataatcg gggcgtcgac ctggcgaacc
    28261 atcatatatt ccagcatcgt gccgttggtc accagaatcg gggctggctc cagacgcatc
    28321 ttttcacggg ataagacttc atttggtcta ttatcctgca ctgttttcat ttttgcgtgg
    28381 agctcttctg tatttccgtt atacaagcag tagcggattc cagtaccaaa accacgcgtc
    28441 caggcatcca gacgctcgcg ctgtgaatta atcaaggcgt tcaatggata gagaaaaagg
    28501 gctcggacac cgaccaacgg attgttgcca ttttcatgca gttcccggta aagatcttcc
    28561 agcaccggaa ccataaaaca ttccgtttta cctgaacccg taccactggt taccaccact
    28621 gaatgtttct tttccagcaa agctttccaa ctggctaact ggtgtgtaaa aggcttccag
    28681 tcagcaccaa atcggtaccg gccattactc tgattatcca gtgaatcaac cactgctttg
    28741 ctcaacaatg cgttgccttc cgtcaggctg cgcatcgtgt gatttgactc cttccatcca
    28801 aacatttgct ggaataccgg agaagcaagg aatgagcccg gtttcccaca atcagccccc
    28861 atttgttgcg cgagatgttc acgcaactgc gggtttgtaa cgcccatgat actgagcgtt
    28921 gcttcagttg aacgagacaa cgattgttca ataagtgacg aaaaatagcg tgtagtcatc
    28981 cttattcctt tttatttttt agcaacataa cgcaacaggc tgcactggaa gatggcctta
    29041 aaccagcggg aatcgaaatc cctgacttgc ctcaggaaaa atacggcacc gggtttacgg
    29101 tcaaatacag attcaaaaga agcatttccg cttgccaccg ctgcggcgaa gacgggtaac
    29161 caggccacgg aatagcggta atctgcatca ggattaatac caatcactgg atttttttgt
    29221 gacatcatcc attcatacag acgcttacag ccatattcag gccagcgagc ctcactgtgc
    29281 tcacgtaaga gctcctgata ccacccgtgg acacaagatt caggaataga cggagggaga
    29341 tatccgttac ttaaccattt ctcaatttca tcggcatatg tcgggaacac gagccccagc
    29401 cgttgaaaca tattagtaac cagtaatttt tgggtttcct ccggtgcacc tttttggctc
    29461 aggaacaatt tgaaacgctc agatgcagct ttgatctcaa agatggggaa aaactcccat
    29521 aaaataggga actcattctc aatacgactc aggtactctg ctgacatttc aaatttgaag
    29581 agcgacattg ccaatgcctg cggatgctgc accagtgcac gccatacttc aaaggttgcc
    29641 agtggtaaat acccgaattg atcgtacagg ctacgcataa attgccagcc ggaatgggca
    29701 ggatcgttag ccatctgtcc gagaacccgt gtgatggtat taacttctgc ctgtggatta
    29761 aaaagctggg tcgctttctg caatgagcgg atgttgcttt cttctaccgg taacgaagat
    29821 tcgccacgaa tgaagcaggg acgaaaagcc atctcttcac ccggtttagg gacaaccaac
    29881 cacggtccat ttttgttaac gattgagctc aactcatatt caccgacagg cactccctcg
    29941 ctcattctag atgctaaggg aatggatttc ctctcgggtt cacttaacag catgataacg
    30001 ggggaaggaa tctgcccagc acgatggttc actgactggc tgagtaatag ttctttttca
    30061 taatcatagc gcaggctgta tttatagcga cgaatttgcc aggccatgac cacacctgcc
    30121 cccgtaatac gcatctcaac aacctgatca atacctgcct caagtgaaat caggttctca
    30181 atatgttcgc gcagactata gagattcaat tcaacaggac gatcgctcaa cgccgtataa
    30241 caccattcat gccatgcctg taatccacta acggagcgca aatacaactg caatgtatat
    30301 tttgtcggct caccatttct ggagaacaga aacgctctgg aacccagcag atcatgcagc
    30361 gtaatgtttt tatccagagg acgtccgttt acatcaagag cgagacagcc tttagcggga
    30421 aaaggtaact caatgtcgat ggggtcagcc gccaaattag gcagaacttg taatgtaata
    30481 aatgccggag gtaccccttc cgctttaagt tgtatctctg ttcgcccctc agtacgctta
    30541 cgtgtagcct ccaatgtttt atctttaagt tttgctacac aaggatgctg tgtggtgatt
    30601 acgatagtgc cttcatttgc gtgcattcca ccttttatct caatactgaa atcagcaggc
    30661 aagataccga ttttgcgacg caagagcgtt tcattattac tattacggat agaaagatat
    30721 tgagccccca gcatctcttg tatatgacat tgctcaattg aaattccgct caggtaacgc
    30781 gttacatcaa tgctttgaat atctggtagt gtagaaatga cttttggtac accaataaat
    30841 gtctcatctg gatgacaaac ccagcttgca cgctttccat ttagagcaaa tcttccgatg
    30901 ctgatttgtt ctcttccggt gcgaatccga taagtttcat ttgctgtaac tgtaacgtcc
    30961 tgacatccct taacaggcag cgctttaagt cctaatacgt ttactgcact cgattggcca
    31021 tcaaaaccag caacagtact attgtcccga gggagcacaa tcagaacatc gctgctttgc
    31081 acactacagg aggcctgtcc ctgtaacagc cattgatctg catcaacgat gaaggttaac
    31141 ggcacctcac caatggcaat ttcactgtca tcaagtttaa tgctcccaac aatcatccct
    31201 ccagcacgag ccactaacga caaacctgcc gatggatttt gcctgacaaa tctcacttcg
    31261 cttttacgta atcgaactgt tgcctgtctg ttttccaggg aagcataagc tggcccaagg
    31321 ccagagactt cctcaccatc ttcacaaatg gcaagttcaa agcgcgtagt tgacggctcg
    31381 cttgttacag gaaaggaaac ttcgtcaggg agagatacta tcgccctaag ctcatctgga
    31441 tgcttttctg accaatagaa ttggcaacgc gtactcttat ttttttgtag tcgaggctta
    31501 gcctctacag aggctgcgca tagcagtcca ttcaaaaaat gcgttcctgt ttcatcatct
    31561 aacggtattg gaaactcgct tcgccaggta gggtgaacct tatcaagctg ttgtactggt
    31621 tctttgtgat ttattaggtt gtgagtaagt actaaggaat tgaggttatc agccatatga
    31681 ctgataagtt ctacagaagt atcttcgcta aaaacagttg gcagcgctga tttttctatg
    31741 acggctcttg ccagcgaaag ggcagaaaaa cctgactgct ttgcttgctc atattgtcca
    31801 aggatcctgg agaaaacagc cagaaagcga ctgtctgatt ctttgagtaa ccggaaaggt
    31861 aaaccacctt cactgaataa tgttccgaga aaattgcgtc gttcagattc ataaaatcga
    31921 attgggcgca gccagtaatc gtccatccct ttcggtacga tggtaccaag ctctgtggcg
    31981 gtaaatgaaa tccctatttt tttatatata gggtcccacg tccatccgca ttgccgttca
    32041 taatccctgc ggtaccactc agagcaaaaa agaacaaaac aggcagcaaa gtccttatta
    32101 catatattag attgtgattg accgactgca cggagtagct gcgttaattg gttatattca
    32161 tcattggttg catgatattc ataaagagga cggccatccg gttgtctcaa ccctctaaga
    32221 ttcacaaagt tacttagcca ggaagtacat tttacccatg aaatg
//
LOCUS       DAPGOF010000042        31877 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.042, whole genome shotgun sequence.
ACCESSION   DAPGOF010000042 DAPGOF010000000
VERSION     DAPGOF010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31877
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.042"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            47..122
                     /locus_tag="RHV13_001653"
     tRNA            47..122
                     /locus_tag="RHV13_001653"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:80..82,aa:Val,seq:tac)
     gene            126..201
                     /locus_tag="RHV13_001654"
     tRNA            126..201
                     /locus_tag="RHV13_001654"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:159..161,aa:Lys,seq:ttt)
     gene            348..423
                     /locus_tag="RHV13_001655"
     tRNA            348..423
                     /locus_tag="RHV13_001655"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:381..383,aa:Lys,seq:ttt)
     gene            457..532
                     /locus_tag="RHV13_001656"
     tRNA            457..532
                     /locus_tag="RHV13_001656"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:490..492,aa:Lys,seq:ttt)
     gene            563..688
                     /locus_tag="RHV13_001657"
     ncRNA           563..688
                     /ncRNA_class="other"
                     /locus_tag="RHV13_001657"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            966..2009
                     /gene="nadA"
                     /locus_tag="RHV13_001658"
     CDS             966..2009
                     /gene="nadA"
                     /locus_tag="RHV13_001658"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HDV1241201.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDHTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQSIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            2047..2766
                     /gene="pnuC"
                     /locus_tag="RHV13_001659"
     CDS             2047..2766
                     /gene="pnuC"
                     /locus_tag="RHV13_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HDV1241202.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(2763..3704)
                     /gene="zitB"
                     /locus_tag="RHV13_001660"
     CDS             complement(2763..3704)
                     /gene="zitB"
                     /locus_tag="RHV13_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HDV1241203.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPLMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(3818..4198)
                     /locus_tag="RHV13_001661"
     CDS             complement(3818..4198)
                     /locus_tag="RHV13_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HDV1241204.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHPDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            4514..5566
                     /gene="aroG"
                     /locus_tag="RHV13_001662"
     CDS             4514..5566
                     /gene="aroG"
                     /locus_tag="RHV13_001662"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HDV1241205.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            complement(5732..6484)
                     /gene="gpmA"
                     /locus_tag="RHV13_001663"
     CDS             complement(5732..6484)
                     /gene="gpmA"
                     /locus_tag="RHV13_001663"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDV1241206.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(6686..7726)
                     /gene="galM"
                     /locus_tag="RHV13_001664"
     CDS             complement(6686..7726)
                     /gene="galM"
                     /locus_tag="RHV13_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HDV1241207.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLLPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALTSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPLDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            complement(7720..8868)
                     /gene="galK"
                     /locus_tag="RHV13_001665"
     CDS             complement(7720..8868)
                     /gene="galK"
                     /locus_tag="RHV13_001665"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HDV1241208.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            complement(8872..9918)
                     /gene="galT"
                     /locus_tag="RHV13_001666"
     CDS             complement(8872..9918)
                     /gene="galT"
                     /locus_tag="RHV13_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HDV1241209.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESHDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            complement(9928..10944)
                     /gene="galE"
                     /locus_tag="RHV13_001667"
     CDS             complement(9928..10944)
                     /gene="galE"
                     /locus_tag="RHV13_001667"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDV1241210.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            complement(11206..12678)
                     /gene="modF"
                     /locus_tag="RHV13_001668"
     CDS             complement(11206..12678)
                     /gene="modF"
                     /locus_tag="RHV13_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HDV1241211.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDAGFYHYVLTKIN"
     gene            complement(12746..13534)
                     /gene="modE"
                     /locus_tag="RHV13_001669"
     CDS             complement(12746..13534)
                     /gene="modE"
                     /locus_tag="RHV13_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HDV1241212.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            13663..13812
                     /gene="acrZ"
                     /locus_tag="RHV13_001670"
     CDS             13663..13812
                     /gene="acrZ"
                     /locus_tag="RHV13_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HDV1241213.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            13979..14752
                     /gene="modA"
                     /locus_tag="RHV13_001671"
     CDS             13979..14752
                     /gene="modA"
                     /locus_tag="RHV13_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV1241214.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFIIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            14752..15441
                     /gene="modB"
                     /locus_tag="RHV13_001672"
     CDS             14752..15441
                     /gene="modB"
                     /locus_tag="RHV13_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HDV1241215.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            15444..16502
                     /gene="modC"
                     /locus_tag="RHV13_001673"
     CDS             15444..16502
                     /gene="modC"
                     /locus_tag="RHV13_001673"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HDV1241216.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(16503..17321)
                     /gene="ybhA"
                     /locus_tag="RHV13_001674"
     CDS             complement(16503..17321)
                     /gene="ybhA"
                     /locus_tag="RHV13_001674"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HDV1241217.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYRLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            17476..18471
                     /gene="pgl"
                     /locus_tag="RHV13_001675"
     CDS             17476..18471
                     /gene="pgl"
                     /locus_tag="RHV13_001675"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HDV1241218.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(18512..19465)
                     /locus_tag="RHV13_001676"
     CDS             complement(18512..19465)
                     /locus_tag="RHV13_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV1241219.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFS
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            19649..20701
                     /locus_tag="RHV13_001677"
     CDS             19649..20701
                     /locus_tag="RHV13_001677"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HDV1241220.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIIPSVGYGNINIEHPSGALDVHLTNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            20777..22210
                     /locus_tag="RHV13_001678"
     CDS             20777..22210
                     /locus_tag="RHV13_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HDV1241221.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            22393..24654
                     /locus_tag="RHV13_001679"
     CDS             22393..24654
                     /locus_tag="RHV13_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HDV1241222.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGIAALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAGNGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(24795..26078)
                     /locus_tag="RHV13_001680"
     CDS             complement(24795..26078)
                     /locus_tag="RHV13_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HDV1241223.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYYASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQRKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTVKPWGDAVISNRPFAGNTGSVD
                     DSDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(26230..26706)
                     /locus_tag="RHV13_001681"
     CDS             complement(26230..26706)
                     /locus_tag="RHV13_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HDV1241224.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(26765..28054)
                     /gene="bioA"
                     /locus_tag="RHV13_001682"
     CDS             complement(26765..28054)
                     /gene="bioA"
                     /locus_tag="RHV13_001682"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HDV1241225.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIIHPQQLQRLTAAVNRAVQDETFFCQ"
     gene            28141..29181
                     /gene="bioB"
                     /locus_tag="RHV13_001683"
     CDS             28141..29181
                     /gene="bioB"
                     /locus_tag="RHV13_001683"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HDV1241226.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMA
                     PDTDEYYNAAAL"
     gene            29178..30332
                     /gene="bioF"
                     /locus_tag="RHV13_001684"
     CDS             29178..30332
                     /gene="bioF"
                     /locus_tag="RHV13_001684"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HDV1241227.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFIHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     TCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTSAHEMQDIDRLLEVLHGNG"
     gene            30319..31074
                     /gene="bioC"
                     /locus_tag="RHV13_001685"
     CDS             30319..31074
                     /gene="bioC"
                     /locus_tag="RHV13_001685"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HDV1241228.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRQSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLVWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDQRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIVRE"
     gene            31067..31744
                     /gene="bioD"
                     /locus_tag="RHV13_001686"
     CDS             31067..31744
                     /gene="bioD"
                     /locus_tag="RHV13_001686"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HDV1241229.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQRNSSLQLDYETVNPYTFAEPTSPHIISAQEGRPIESSVMSSG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
BASE COUNT         7573 a         8010 c         8461 g         7833 t
ORIGIN      
        1 tacggtaggt tcggtggaag tagtttgtag tatccagcgc agtatcgggt gattagctca
       61 gctgggagag cacctccctt acaaggaggg ggtcggcggt tcgatcccgt catcacccac
      121 cactcgggtc gttagctcag ttggtagagc agttgacttt taatcaattg gtcgcaggtt
      181 cgaatcctgc acgacccacc agttttaaca tcaaactcag atgttaagcg tgaaggataa
      241 cgttgcgcca gcaacggccc gaagggcgag cgaagcgagt catcctgcac gacccaccaa
      301 tcttaaagat tggccccgag taaaaatctt tcaggtaaca cccgtatggg tcgttagctc
      361 agttggtaga gcagttgact tttaatcaat tggtcgcagg ttcgaatcct gcacgaccca
      421 ccatcctgaa tgattaaagc atcataatcc cgcaaggggt cgttagctca gttggtagag
      481 cagttgactt ttaatcaatt ggtcgcaggt tcgaatcctg cacgacccac caatgtaaaa
      541 aagcgcccta aaggcgcttt tttgctatct gcgatactca aagattcgaa cctgcagcag
      601 gtttgagttg agcgcagcga aacaacggag ccgctcgcgg cgacggcccg aagggcgagc
      661 gaagcgagtc atcctgcacg acccaccaat gtaaaaaagc gccctaaagg cgattttttg
      721 ctattcaggc atctctcaat ttcaattctt gaccctgatg acatactacg cgcttgctgc
      781 gtaaacaact ttacgtcgga ggaataagac gcaaagtgtg gcattcggca tctcatacgt
      841 aattcgagtg acttttcccc accattcgac tatcttgttt agcatataaa acaaacttca
      901 ccgataacag cgaatatcac gctaatgtcg gttttaacgt taagcctgta aaacgagatg
      961 gtaagatgag cgtgatgttt gatccagaca cggcgattta tcctttcccc ccgaagccga
     1021 cgccgttaag cattgatgaa aaagcgtatt accgcgagaa gataaaacgt ctgctaaaag
     1081 aacgtaatgc ggtgatggtt gcccactact ataccgaccc cgaaattcaa caactggcag
     1141 aagaaaccgg tggctgtatt tctgattctc tggaaatggc gcgcttcggt gcaaagcatc
     1201 ccgcttctac gttgttagtc gctggggtga gatttatggg agaaaccgcc aaaattctca
     1261 gtccggaaaa aacaattctg atgccgacac ttcaggctga atgttcactg gatctcggct
     1321 gccctgttga agaatttaac gcattttgcg atgcccatcc cgatcatact gtcgtcgtct
     1381 acgccaacac ttctgctgcg gtaaaagcgc gcgcagattg ggtggtaact tcaagcattg
     1441 ccgtcgaact tattgatcat ctggatagtt tgggtgaaaa aatcatctgg gcacccgaca
     1501 aacatctggg gcgttacgtg caaaaacaga cgggcgcaga cattttatgc tggcagggtg
     1561 cctgtattgt gcatgatgaa tttaagactc aggcgttaac ccgcttgcaa gaagaatacc
     1621 cggatgctgc catactggtg catccagaat caccacaagc tattgtcgat atggcggatg
     1681 cggtcggttc caccagtcaa ctgatcgctg ctgcgaaaac attgccacat cagcggctta
     1741 tcgtggcaac cgatcggggc attttctaca aaatgcagca ggcggtgcca gataaagagt
     1801 tactggaagc accaaccgca ggtgagggtg caacctgccg cagctgcgcg cattgtccgt
     1861 ggatggccat gaatggcctt cagtccatcg cagaggcatt agaacaggaa ggaagcaatc
     1921 acgaggttca tgttgatgaa aggctgcgag agagggcgct ggtgccgctc aatcgtatgc
     1981 tggattttgc ggctacacta cgtggataac gaataataag gcgtaacgtt acgctttggg
     2041 ggaaagatgg atttttttag tgtgcagaat atcctggtac atataccaat aggggcaggc
     2101 ggttatgatc tctcatggat cgaagcggta ggcacgatcg ccgggttgct gtgtattggc
     2161 cttgccagtc tggagaagat cagcaactac ttctttggcc tgatcaacgt caccttgttt
     2221 ggcattattt tctttcagat tcagctgtat gccagcctgc tattacaggt gtttttcttt
     2281 gccgcgaata tttacggctg gtatgcgtgg tcgcgacaaa ccagtcagaa cgaggcggag
     2341 ttgaaaattc gctggttgcc attgccgaag gcactcagct ggttggcggt ttgcgttgtt
     2401 tcgattggtc tgatgacggt atttatcaat ccggtgtttg catttttgac ccgcgtggca
     2461 gtcatgatca tgcaagcatt aggattacag gttgtgatgc ctgaactgca accggacgct
     2521 ttcccgttct gggattcttg catgatggtg ttatctatcg tggcaatgat tctgatgacg
     2581 cgtaagtatg tggaaaactg gctgttgtgg gtgattatta acgtgattag cgtcgttatt
     2641 tttgcacttc agggcgttta cgccatgtct ctggagtaca tcatcctgac ctttattgcg
     2701 ctcaacggca gccggatgtg gatcaacagc gcacgtgaaa gaggctcacg cgcgctgtcc
     2761 cattaatggt gatgatgtga atggccagac accccttcat tcaggtggca gtctggccca
     2821 tgacaaggtt gatattccat ctgaatggtg gcgtgctcaa tctgatagtg atccatcagg
     2881 tagtgttgga tctgatccaa caaggcatcg tgatcgtgtg gcgggatcac ctgcacatgc
     2941 agtgtcatca acggcttctc gcctaccatc catacatgta catggtgcac attgcggact
     3001 tccgggattt cccggcacat acgacgcttc agttcagcga tatccagcga taccggtgca
     3061 ccttcaagta attcattcac gctatctttc aacagtcgcc acgcgctacg cagaaccaga
     3121 agcgacacca gtatcgagag aatggggtca gcaggtgtcc agccggtcca gataataatc
     3181 aacgcggcaa taatagcccc aaccgaaccc agcaggtccc cgagcacatg cagtgccgct
     3241 gctcgcacgt tgaggttttt ctcttcgctg ccgtgatgga gtaaccagaa agaaagtata
     3301 tttgccagca acccagccac ggcaattgcc atcatcatgc cgccctcgac cggacgcggc
     3361 gtgcggaagc gttctatcgc ctcccagaca atcaaaatgg taattaccac cagggcgatg
     3421 gcattcacaa acgccgcgag cgtggtcagt cttagccaac cgaaagtgtg gcgaatggtg
     3481 ggaggacgac gtgaaaattg cacggcgagt agggcgaaaa gcagggcggc agtatcggtc
     3541 aacatatgac ccgcatcggc cagcaaagcc agagaaccag aaagaaaacc accaatgact
     3601 tctaccagca taaacccggc agtcacgccg aaagcataca acaagcgacg agcattatta
     3661 tcttctggca ggtgtgagga cgtgtgtgag tgtgagtgcg ccatgacgag tcatccctta
     3721 attattctca cttaacttta tgacatcata ctgcttttag aagggaaaaa ttaaaaggga
     3781 gagactccgc tctcccatta ttggctattt tgcagggtta ctgcgtggta ccgtcggttt
     3841 tggtatcgac atcattattg atgccatcac cggtttgtac ctttttattg atatccgggc
     3901 agcgaccatc tttgcacatg gtgttcttgt gctcttcatc tttggtcatg ccgtcattgt
     3961 tcattgaaga accatccgga tgcagcatag tgccgccaga accggtattt accccgttat
     4021 tgtcgacgtt atttggcgcg acattttcac gggcgtcagg ggctacctgg cccgcatcag
     4081 ctgcggcgtt tgcctggccg ttattggttt gcgctccgct atcggcagcc agtgcggcac
     4141 cgctggcaag gcttagagtg gcagtcagaa atagtgtggc cagttttgtc attttcatag
     4201 gatgctcctg ttatggtcgt tatgtcggat aacctcttcc aacagtgcat ttgcaggtga
     4261 atataaggca ttggtttaag atttcagcca ggttatgaaa cgcagcagag aatcttgaaa
     4321 taattaacaa acaaaggagt tacagttaga aattgtagga gagatctcgt ttttcgcgac
     4381 aatctggcgt ttttcttgct aattccagga ttaatccgtt catagtgtaa aaccccgttt
     4441 acacattctg acggaagata tagattggaa gtattgcatt cactaagata agtatggcaa
     4501 cactggaaca gacatgaatt atcagaacga cgatttacgc atcaaagaaa tcaaagagtt
     4561 acttcctcct gtcgcattgc tggaaaaatt ccccgctact gaaaatgccg cgaatacggt
     4621 tgcccatgcc cgaaaagcga tccataagat cctgaaaggt aatgatgatc gcctgttggt
     4681 ggtgattggc ccgtgctcaa ttcatgatcc tgtcgcggct aaagagtatg ccactcgctt
     4741 gctggcgctg cgtgaagagc tgaaagatga gctggaaatc gtaatgcgcg tctattttga
     4801 aaagccgcgt accacggtgg gctggaaagg gctgattaac gatccgcata tggataacag
     4861 cttccagatc aacgacggtc tgcgtatagc ccgtaaattg ctgcttgata ttaacgacag
     4921 cggtctgcca gcggcgggtg agtttctcga tatgatcacc ccacaatatc tcgctgacct
     4981 gatgagctgg ggcgcaattg gcgcacgtac caccgaatcg caggtgcacc gcgaactggc
     5041 atcagggctt tcttgtccgg tcggcttcaa aaatggcacc gacggtacga ttaaagtggc
     5101 tatcgatgct attaatgccg ccggtgcgcc gcactgcttc ctgtccgtaa cgaaatgggg
     5161 gcattcggcg attgtgaata ccagcggtaa cggcgattgc catatcattc tgcgcggcgg
     5221 taaagagcct aactacagcg cgaagcacgt tgctgaagtg aaagaagggc tgaacaaagc
     5281 aggcctgcca gcacaggtga tgatcgattt cagccatgct aactcgtcaa aacaattcaa
     5341 aaagcagatg gatgtttgtg ctgacgtttg ccagcagatt gccggtggcg aaaaggccat
     5401 tattggcgtg atggtggaaa gccatctggt ggaaggcaat cagagcctcg agagcgggga
     5461 gccgctggcc tacggtaaga gcatcaccga tgcctgcatc ggctgggaag ataccgatgc
     5521 tctgttacgt caactggcga atgcagtaaa agcgcgtcgc gggtaagggt taattgtcgg
     5581 atgcaccgcc agagtggcgt atccgatgaa tcaccatagc cctgataaga cgcgcagcgt
     5641 cgcatcaggc aatgtgctcc attgttagca acgacaaaca aaaaagccga ctcacttgca
     5701 gtcggctttc tcatttttaa acgaatgacg tttacttcgc tttaccctgg ttcgcaacgg
     5761 ctgctgcttt cgccgcgatc tcgtcagcat tacccagata atagcgtttc agcggtttga
     5821 agttctcgtc aaactcataa accagcggca cgccggtcgg gatattcagc tcaagaatct
     5881 cttcttcgct catgttatcg agatatttca ccagcgcacg taacgagtta ccgtgtgcag
     5941 cgatgattac gcgctcaccg ctcttcatac gcggtagaat agtttcattc cagtaaggga
     6001 tcacgcggtc aatggtcagc gccaggcttt ccgtcagcgg cagttctttc tcgctcagtt
     6061 tcgcgtaacg gggatcgtga cccggataac gctcatcatc tttagtcagt tccggcggag
     6121 tcactgcaaa accacgacgc cactgtttca cctgctcgtc gccatacttt tcagcagttt
     6181 ccgctttgtt cagaccctgc aacgcaccgt agtgacgttc gttcagtttc caggatttct
     6241 caacgggcag ccatgcctga tccagttcgt ccagcacatt ccacagggta tggatagcgc
     6301 gtttcagcac agaggtgtaa gcaaagtcaa agctgtaacc ttcctctttc agcagcttac
     6361 ctgctgcttt tgcttcgctt acgcctttct cagacagatc cacgtcgtac caaccggtga
     6421 aacggttttc tttgttccac tgactttcgc catgacgaac cagaaccagc ttagttacag
     6481 ccatatactt actcctcaaa tcatctttta atgataataa ttctcattat attgccgtga
     6541 cgaagcaaca gcaatgctta cgcataacca tagcgaaaat agtgtcgcag tgtaaggttg
     6601 ttgtgaatat tgagttgcaa atatgtcggt gtttgctggt gatttgaaca atatgagata
     6661 aagccctcat gacgagggct taacattact cagcaataaa ctgatattcc gtcaggctgg
     6721 aatactcttc gccaggacgc aggaagcagt ccggttgcgg ccattcaggg tggttcgggc
     6781 tgtccggcag aaactcgctt tccagcgcca gcccttgcca gtcggcgtaa ggttcggttc
     6841 cccgcgacgg tgtgccgccg aggaagttgc cggagtagaa ttgcagagcc ggagcggtgg
     6901 tgtagacctt cagctgcaat ttttcatctg ctgaccagac atgcgccgcc actttcttgc
     6961 catcgccttt ggcctgtaac aagaatgcgt gatcgtaacc tttcactttg cgctgatcgt
     7021 cgtcggcaag aaactcactg gcgatgattt tggcgctgcg gaaatcaaaa gacgttccgg
     7081 cgacagattt caggccgtcg agcggaatgc cgccttcatc aaccggcaga tattcgtccg
     7141 ccagaatctg caacttgtga ttgcgcacgt cagactgctc gccgtcaaga ttgaaataga
     7201 cgtgattagt catattcacc gggcaaggtt tatcaactgt ggcgcgataa gtaatggaga
     7261 tacggttatc gtcggtcaga cgatattgca ccgtcgcgcc gagattaccc gggaagccct
     7321 gatcgccatc atctgaagtc agggcaaaca gcacctgacg atcgttctgg ttcacaatct
     7381 gccagcgacg tttgtcgaac ccttccgggc cgccgtgcaa ttggttgacg ccctgacttg
     7441 gcaacagcgt cacggtttca ccgtcaaagg tataacggct attggcgatg cggttagcat
     7501 aacgaccaat agaggccccc agaaacgcgg cctgatcctg atagcattcc gggctggcac
     7561 agccgagcag cgcctcgcgg acgctgccat cggaaagcgg aatacgggct gaaagtaaag
     7621 tcgcacccca gtccatcagc gtgactacca tccctgcgtt gttacgcaaa gttaacagtc
     7681 ggtacggctg accatcgggt gccagtgcgg gagtttcgtt cagcactgtc ctgctccttg
     7741 tgatggttta caaacgtaaa aagtctcttt aatacctgtt tttgcttcat attgttcagc
     7801 gacagcttgc tgtacggcag gcaccagctc ttccgggatc agcgcgacga tacagccgcc
     7861 aaatccgccg ccggtcatgc gtacgccacc tttgtcgcca atcacagctt tgacgatttc
     7921 taccagagtg tcaatttgcg gcacggtgat ttcgaaatca tcgcgcatag aggcatgaga
     7981 ttccgccatc aactcgccca tacgtttcag gtcgccttgc tccagcgcgc tggcagcttc
     8041 aacggtgcgg gcgttttcag tcaggatatg ccgcacgcgt ttcgccacga ttgggtccag
     8101 ctcatgtgca acagcgttga actcttcaat ggtgacatcg cgcagagctg gctgctggaa
     8161 gaaacgcgca ccggtttcgc actgttcacg acgggtgttg tattcgctgc caaccagggt
     8221 acgtttgaag ttactgttga tgatgacgac agccacacct ttcggcatgg aaactgcttt
     8281 agtccccagt gagcggcagt caatcagcaa ggcatgatct ttcttgccga gtgcggaaat
     8341 tagctgatcc atgatcccgc agttacagcc aacaaactgg ttttctgctt cctgaccgtt
     8401 aagcgcgatt tgtgcgccgt ccagcggcag atgataaagc tgctgcaata cggttccgac
     8461 cgcgacttcc agtgaagcgg aagaacttaa cccggcaccc tgcggcacat tgccgctgat
     8521 caccatgtcc acgccgccga agctgttgtt acgcagttgc agatgtttca ccacgccacg
     8581 aacgtagttc gcccattgat agttttcatg cgcgacaatg ggcgcatcga gggaaaactc
     8641 gtcgagctga ttttcataat cggctgccat tacgcgaact ttacggtcat cgcgtggcgc
     8701 acagctgatc acggtttgat aatcaatcgc gcagggcaga acgaaaccgt cgttgtagtc
     8761 ggtgtgttca ccaatcaaat tcacgcggcc aggcgcctga atggtgtgag tggcagggta
     8821 gccaaatgcg ttggcaaaca gagattgtgt tttttctttc agactcattt cttacactcc
     8881 ggattcgcga aaatggatat cgctgactgc gcgcaaacgc tctgctgcct gttctgcggt
     8941 caggtctcgc tgggtctctg ccagcatttc ataaccaacc ataaatttac gtacggtggc
     9001 ggagcgcagc agaggcggat aaaagtgcgc gtgcagctgc cagtgttgat tctcttcgcc
     9061 attaaatggt gcgccgtgcc agcccataga gtaggggaag gagcactgga agaggttgtc
     9121 ataacgactg gtcagctttt tcaacgccag cgccaaatcg ctgcgctggg cgtcggtcaa
     9181 atcggtgatc cgcaaaacgt gggctttggg cagcagtagc gtttcgaacg gccaggcagc
     9241 ccagtaaggc acgacggcta accagtgttc ggtttcgaca acggtacggc taccgtctgc
     9301 cagctcgcgc tgaacataat ccaccagcat tggtgatttc tgttcggcaa aatattcttt
     9361 ttgcaggcgg tcttcgcgct cagcttcgtt aggcaggaag ctatttgccc aaatctgacc
     9421 gtgcggatgc gggttagagc agcccatcgc agcgcctttg ttttcaaaaa cctgcaccca
     9481 tgggtacgtt ttccccagtt ctgcggtttg ctcctgccag gttttgacga tttccgtcaa
     9541 tgctgcaacg ctgagctctg gcagcgtttt actgtgatcc ggtgaaaagc agatcacccg
     9601 gctggtgccg cgcgcgctct ggcaacgcat caaaggatcg tgactttctg gcgcatctgg
     9661 cgtgtcagac atcaaggccg caaagtcatt agtgaaaacg taagtcccgg tgtaatcggg
     9721 gtttttatcg cctgtcaccc gcacattacc tgcgcagagg aagcaatctg gatcgtgcgc
     9781 aggtaacacc tgtttggctg gcgtttcctg cgccccctgc caggggcgct tagcgcggtg
     9841 cggtgaaacc agaatccatt gcccggtgag cgggttgtag cggcgatgtg gatgatcaac
     9901 gggattaaat tgcgtcatgg tcgttcctta atcgggatat ccctgtggat ggcgtgactg
     9961 ccagtgccag gtgtcctgcg ccatttcatc gagtgtgcgc gttacgcgcc agttcagttc
    10021 acggtcggct ttgctggcgt ccgcccagta ggccggaagg tcgccctcgc gacgcggtgc
    10081 aaaatgatag ttaactggtt tgccgcaggc tttgctgaag gcattaacca cgtccagcac
    10141 gctgctgcct acgccagcac cgaggttgta gatgtgtacg cctggcttgt tcgccagttt
    10201 ttccatcgcc acgacgtgac cgtccgccag atccattacg tggatgtaat cgcgtacgcc
    10261 agtaccgtct tcggtcggat aatcgttacc aaaaatcgcc agcgagtcgc gacggcctac
    10321 agcaacctgg gcgatgtatg gcatcaggtt attcggaatg ccttgcggat cttcgcccat
    10381 atcgcccgac ggatgcgcgc caaccgggtt gaagtagcgc agcagggcaa tgctccagtc
    10441 cggctgggct ttttgcagat cggtgaggat ctgttccacc atcagcttgc ttttgccgta
    10501 agggctttgc ggtgtgccgg tcgggaagct ttcaacgtat ggaattttgg gctgatcgcc
    10561 ataaacggtg gcggaggagc taaaaataaa gtttttgacg ttagcggcgc gcatggcgct
    10621 aatcaggcgc agagtaccgt tgacattgtt gtcgtaatat tccagcggtt tttgtaccga
    10681 ttcgccaacg gctttcagcc cggcgaagtg gatcacggtg tcgatagcgt gatcgtgcag
    10741 gatctcggtc atcaacgctt cgttacggat atcgccttca acaaacgttg gatgtttgcc
    10801 gcctaaacgc tcgataacag gcagtacgct gcgcttactg ttacagaggt tatcaagaat
    10861 gatgacatca tgaccgtttt gcagtaattg cacacaggta tgacttccaa tgtaaccgct
    10921 accaccggtg accagaactc tcataattcg ctccattagg cttatggtat gaaataacca
    10981 tagcataaca aagatgcgaa aagtgtgaca tggaataaat tagtggaatc gtttacacaa
    11041 gaatttagcc gttttttatg tgcgattaag tgattataaa acagagggtt tatgaatgat
    11101 tgcgcttttt atctgaaaaa aagactcggt ttcatgcctg gatgcgtctg accgtgggcc
    11161 ggataacgcg tttacgccac atccggcacg gttagcacta ccgactcaat ttatttttgt
    11221 cagcacatag tgatagaacc cagcgtccgg cacgaactcc agtcgatggg taatacaagc
    11281 gggtgcatct tcagcatggt gcgaaacaaa caacaattgc gtttcacctt cgctaatcag
    11341 cacatcaaca aaacggcgga taagctggcg attgagcgga tcaagcccct gtagtggttc
    11401 atcgagaata agcaacgtcg gatgtttcac cagtgcgcgg acgatcagcg ccagacgctg
    11461 ctgtccccag gaaagactat ggaacggagc gtcagccgtg cgtttatcaa tgccgagaat
    11521 atccagccac tgctgcacca gtttttgctg gcgatccgaa acggcctgat aaatgccaat
    11581 cgaatcaaaa tagccagaaa gaatcacatt acgcacggta gtgctgaccc ggtaatccag
    11641 atgcaaacta ctgctgacgt aaccgatatg ctttttgatg tcccagatgg tttcgccgct
    11701 gccgcgacgt cggccgaaaa gcgtcaaatc gttgctgtaa ccttgcggat gatcgccagt
    11761 aaccaggctt aataacgtcg attttcctgc accatttggc ccgacaattt gccagtgttc
    11821 acctggattc acctgccagc taaggttatt aagaatgggg cgatcgttat aagaaaccac
    11881 gccattgttc agcacaatgc gcggttcgtt ggcgggtaag gcgtgacgtg ctgaaggttc
    11941 atccggctcc ggcagttgca caccttcaag ctgttcacta tgcgccagtt gcgcgacgag
    12001 tgcttgttgg agcagttcct ctttagcgcc cgtttccgct aacgtgcaat ccgccagcac
    12061 gccagcaaac tggacaaact ccgggatctc atcgaagcga ttgagcacca gtaccagagt
    12121 aataccggac tgatgtaacg aggcgagtcg ctcagccagc tgctgacgtg aggcaacatc
    12181 caggccatcg aacggctcat caagaatcaa caagtcaggc tccgacatca gcgcctgaca
    12241 cagcagggtt tttcgcgtct cgccagtgga aaggtattta aagcgtcggt cgaggagggc
    12301 ggtaataccg aactgctgcg ccagttgcat gcaacgcggt gcatccttta cttcatcctg
    12361 aatgatctca gccgtagtgc gtccggtgtc atcttcgcca gggccgagca tatcggtgtt
    12421 attccgctgc cattcgtcgc tgacgagttt ttgcaattgc tcgaaggaga gacgagttat
    12481 gtgggaaaac tggctttgcc gttcaccttt caaaagcgga agttcccccg ccagcgcgcg
    12541 ggccagggcc gatttcccgc ttccattcga accgacaaac gcccaactat cacccgcgtt
    12601 taacgttagc tgaggcaatt gcagcgtttt tgtgtcgcta agacgaaacg tgccttgcaa
    12661 aatttgcaac gatgacattt tatatcccat tttgtgcagc gattactgac agggatacgt
    12721 gtttcataac aaattgtcaa cacgcttagc acagcgtggc gataatcacg ctgtcggcat
    12781 taaagtaggc cgtgacattc tgtccttgct gaagagaagt cgcttcattt accggcactg
    12841 tggcgcacag tgtttgcccg tccggtagcg ccattaatac ttcgcactgc tctgcgccgc
    12901 gctcaatatg actaataata cccggtaatt ggttgtcagc gttttgcgcg accgcttcgt
    12961 cctgagtaat gcctacccac ggcgctttta gcaatatcaa cacttctttg ccttcatcca
    13021 gccccagacg cgcgccgctt tgtgcggtaa ttgcgacttt caggcgtgtt tttccgtcag
    13081 ccagtaagac atcaacatgc tgttgaacgt catcatgatc gcgggcggtg atggtaccga
    13141 accactggtt acgggcgctg gtttgcagtg aaaaacgtga gatcgcggcc agcaggctgt
    13201 tcagcggcag ggcgtcatcg tcacttaaca catcaaaggc tttttgctgg atttgcgcca
    13261 gtaagtcata gagctgaatc agtcgctgac catagcgggt cagtactgcg ccgccgccac
    13321 ctttaccgcc tgttgcgcgc tcgaccagaa tatgctcact taactgattc atctcgttaa
    13381 tggcatccca ggcgctttta tagctgatac cggcatcttt cgctccctgg ctaatggaac
    13441 cggaaagcgc aatgtgtttt agtagcgaaa tgcggcgcgg gtcggcgaat aatttttgtt
    13501 ggagcttaag ggtgagaagg atttcggcct gcataacaat gtcctggcaa aagtcttatt
    13561 gtgacggaaa acgaacgcca cgcaaagctg accgcacaaa aggggagtgc ttttctgtgc
    13621 ttagcggtta gaatagtctc atgactatat ctggagttga ccatgttaga gttattaaaa
    13681 agtctggtat tcgccgtaat catggtacct gtcgtgatgg ccatcatcct gggtctgatt
    13741 tacggtcttg gtgaagtatt caacatcttt tctggtgttg gtaaaaaaga ccagcccgga
    13801 caaaatcatt gattccctga atgcccgctt agtcgggcat tttctttttc tcaacttcct
    13861 gcttttcctg ccgatatttt ttcttatcta cctcacaaag gttagcaata actgctggga
    13921 aaattccgag ttattcgtta tattgtcgcc tacataacgt tacattaagg ggttaccaat
    13981 ggctcgtaaa tggttgaact tgtttgccgg ggcggcactc tctttcgctg ttgctggcaa
    14041 tgcactggca gatgaaggga aaatcacggt gttcgccgcc gcatcactga ctaacgcaat
    14101 gcaggacatt gctacgcagt ataaaaaaga gaaaggcgtg gatgtggttt cttctttcgc
    14161 ttcgtcatct actctcgccc gtcagattga agcgggtgcc cctgcggatc tgtttatctc
    14221 tgccgatcag aaatggatgg attatgcggt tgataaaaaa gcgatcgata cggcttcgcg
    14281 ccagacactg ctcggcaata gcctggtcgt tgtagcaccg aaagccagcg agcagaaaga
    14341 tttcatcatc gacagcaaaa ctaactggac ttcgctgctg aatggcggtc gcctcgcggt
    14401 tggcgatccg gaacatgtcc ctgctggcat ttatgcaaaa gaagcactgc aaaaactggg
    14461 cgcatgggat acgctctctc cgaaactggc cccagcggaa gatgttcgtg gggcgctggc
    14521 gttggtcgaa cgcaatgaag cgccgttggg cattgtttac ggttctgacg cagttgccag
    14581 caaaggggta aaagtggttg ccaccttccc ggaagattca cataaaaaag tggaatatcc
    14641 ggttgctgtt gtggaagggc ataacaatgc gacagtgaaa gcattttatg attatctgaa
    14701 gggaccgcag gctgcggaaa tctttaaacg ttacggattt acaaccaagt aatgatactg
    14761 accgatccag aatggcaggc agttttatta agcctgaaag tttcttccct ggctgtgctg
    14821 tttagcctgc cgtttgggat cttttttgcc tggttactgg tgcgatgcac gtttccgggc
    14881 aaagctctgc tcgacagcgt actgcatcta ccgctggtgt taccgcccgt ggtcgtcggt
    14941 tacttattat tagtttcgat gggacggcgc ggatttatcg gtgaacgtct gtatgactgg
    15001 tttggtatta ccttcgcctt tagctggcgc ggcgcggttc tcgctgccgc cgtcatgtcg
    15061 tttccgctga tggtgcgggc aattcgtctg gcgctggaag gggttgatgt caaactggaa
    15121 caggccgcaa gaacactggg ggccgggcgc tggcgcgttt tctttactat cacgttaccg
    15181 ctgacattac cgggcattat tgttggtacg gtactggctt ttgctcgttc tctcggtgag
    15241 tttggtgcaa ccatcacctt tgtgtcgaac attcctggtg aaacgcgaac cattccttct
    15301 gccatgtata ccctgatcca gacccccggc ggggaaagtg gagcggcgag actgtgcatt
    15361 atttctattg cactggcgat gatctccctg ttgatttcag aatggctggc cagaatcagc
    15421 cgtgaacggg cggggcgcta atcatgctgg agctgaattt ttcccagacg ttgggcaacc
    15481 attgcctgac cattaatgaa acgctgcccg ccaatggcat cactgctatc tttggcgtct
    15541 ccggtgccgg aaaaacttcg ctgattaacg ccatcagtgg actgacgcgc ccgcaaaaag
    15601 ggcggattgt cctcaatggg cgggtactaa atgatgccga aaaaggtatc tgcctgacgc
    15661 cggaaaagcg tcgcgttggc tatgtttttc aggatgcgcg gctgttcccg cattacaaag
    15721 tgcgtggcaa tctgcgctac ggcatgtcga aaagtatggt cgatcagttc gataagctgg
    15781 tggcgctttt aggcattgaa ccgttgcttg accgtttacc aggcagcctg tccggaggcg
    15841 aaaaacagcg cgtggcgatt ggtcgggctt tgctgacagc accagaattg ctgttgctgg
    15901 atgaaccgct ggcgtcactg gatattccgc gtaaacgcga actgttgcct tatctgcaac
    15961 gtctgacgcg ggaaatcaac atcccgatgt tgtatgtcag ccattcgctg gatgagatcc
    16021 tccatctggc agacagagtg atggtactgg aaaacggtca ggtgaaagcc tttggcgcgc
    16081 tggaagaagt ctggggtagt agcgtgatga atccgtggct gccgaaagag caacaaagta
    16141 gcattctgaa agtgacggta cttgagcatc atccgcatta cgcgatgacc gcgctggcgc
    16201 tgggcgatca gcatttgtgg gtcaataagc tggacgaacc gctgcaagct gcgctacgta
    16261 tccgcattca ggcttccgat gtttctctgg ttttacaacc gccgcagcaa accagcattc
    16321 gtaacgtatt gcgggcaaaa gttgttaata gttatgacga caacggccag gtggaagtgg
    16381 aactggaagt cggcggtaaa acgctgtggg cgcgtatcag cccgtgggcc agggatgaac
    16441 tggcgatcaa acctggcctg tggctgtacg cgcaaattaa aagtgtgtcg ataaccgcct
    16501 gattaaatca ggtggctata aatgaactgg gcaatgctgt cggtggtgtt atcaccaatc
    16561 acaatgttgg cgcgcgcttt taccgcgtca tcggcgttgc ccatcgccac gcctgtacca
    16621 gcggcttcca gcatactgat atcattaaag ttatcgccga acgccacgac gttttccatc
    16681 gaccagcctt gcgcctcaac ccatttcgtc aaacgtttac ctttgctgtt gccgccgcgt
    16741 gcaatatcaa cctgatcgtg ccaggaccat tcacactcca gtcccagttc atgttcgaca
    16801 tgcttaccaa aatgctgcaa ttgcggcagg tcatcgtgcg tcagggcgaa cttccatacg
    16861 gcgttaactt gttgcgccgt ttcagccaga gaagcgactt gtgtgaaagt cggacgctgt
    16921 tccggcggca gggtttgcgc ccagttagac gtgcgaatga catgcccggt cgggtgctca
    16981 tagaccattg catcatccac atacatcaga ccgtgaatgt ggtgttcatt cagcatctca
    17041 atgagttgca gggctttatt aacgggcatt gggtccgctt ccagcacggt ttttgcatga
    17101 taatcataca aataggtgcc attacagcaa atagcaggtg tatccagcgc cagcgcctga
    17161 taaaaaggat gaatagcgac gtgatggcga cctgtgacga tgattaatcg atagcctgct
    17221 tcgcgagcac gggccagggc ttctatcgat gaaggaagca gggtcttttt cggggtcaat
    17281 aaggtgccgt ctaagtcgag agcaatcacg cgtgtggtca tggcgtattc cagattaagg
    17341 ttaagaattt tcgtctgcgc gaatggtaca ccgataccac tatcggacaa aattctgcgt
    17401 tttaattcag cattcaccgc caaaagcgac taattttagc tgttacagtc agttgctaaa
    17461 tgcaaaggag cattcatgaa gcaaacagtt tatatcgcca gccctgagag ccagcaaatt
    17521 cacgtctgga acctgaatca tgaaggcgca ctgacgctga cacaggttgt cgatgtgccg
    17581 gggcaggtgc agccgatggt ggtcagcccg gacaaacgtt atctctatgt tggtgttcgc
    17641 cctgagtttc gcgtcctggc gtatcgtatc gccccggacg atggcgcact gacctttgcc
    17701 gcagagtctg cgctgccggg tagtccgacg catatttcca ccgatcacca ggggcagttt
    17761 gtctttgtag gttcttacaa tgcgggtaac gtgagcgtaa cgcgtctgga agatggcctg
    17821 ccagtgggcg tcgtcgatgt ggtcgagggg ctggacggtt gccattccgc caatatctca
    17881 ccggacaacc gtacgctgtg ggttccggca ttaaagcagg atcgcatttg cctgtttacg
    17941 gtcagcgatg atggtcatct ggtggcgcag gaccctgcgg aagtgactac cgttgaaggg
    18001 gccggcccgc gtcatatggt attccatcca aacgaacaat atgcgtattg cgtcaatgag
    18061 ttaaacagct cagtggatgt ctgggaactg aaagatccac acggcaatat tgaatgtgtc
    18121 cagacgctgg atatgatgcc ggaaaacttc tccgacaccc gttgggcggc tgatattcat
    18181 atcaccccgg atggtcgtca tttatacgcc tgcgaccgta ccgccagtct gattaccgtt
    18241 ttcagcgttt cggaagatgg cagcgtgttg agtaaagaag gcttccagcc aacggaaacc
    18301 cagccgcgcg gcttcaatgt tgatcacagc ggcaagtatc tgattgccgc cgggcaaaaa
    18361 tctcaccaca tctcggtata cgaaattgtt ggcgagcagg ggctactgca tgaaaaaggc
    18421 cgctacgcgg tcgggcaggg accaatgtgg gtggtggtta acgcacacta accgctgatt
    18481 tacccggcgc agtctctcct gcgccggtgt attaacctat ctcctgtaac gcatgtctct
    18541 ggcgttcgac gatattggtc cacaaattgt ctttatcgtc agtccacaaa ttaatcagca
    18601 aggcaaaaaa gcgttcagct gccggagaaa gcacggcatc tttgcggcga ataatcccca
    18661 atgtccgacg tataacgggt tccaccagtg ggataccaat aatcgaagaa tagggcgcat
    18721 ggggcattgc cagaccagga agcgctgaaa tccccagtcc tgcctccacc agtcctaatg
    18781 acgtcgaaag atgacgcact tcgtaaaacc agtccagctt ccagggcttg tcggctagct
    18841 gttgttctat caacagtcgg ttgccgctgg aggagcgtac gccaatcatt ttgtaaccca
    18901 ctaactcctg ccattctaca agctgctttt tggccaatgg atggtcacgc cgacaggcca
    18961 gcacgaacgg ctcgttgacc agtgggctaa aatcaatgga tgaatttgtg acgttgttca
    19021 tgtttatgcc aaagtctgat tcgttacata gcaccgattc catgcaatta ttcgtgcctt
    19081 gttccagaat ccgcacttta atattgggat acagctcatt aaatttaccg attgccagcg
    19141 gtaaaaaata aaatactgcg gtcggaatac acgctaacgt caccatacca cgatgataag
    19201 cattcatatc acgaatatta aaaagcgttt catcgaattt ctttattaat tcccgcgcct
    19261 ccgggagcaa cctttttcct gcttttgtta acgtaacctt gcgagttgta cgctcaaata
    19321 gttgaatatg taaatcttcc tccatctttt ttattcttcg cgttaatgca ggttgcgtaa
    19381 tgttgagtaa tttagcggca ttgttaaatg aactggactc tgccagtatg acaaatgcct
    19441 tcatacttga taattcatgc ttcattatta ctccggaaaa tggaagcgac gattttgggt
    19501 ggctggtcgt taaaaatttt aactgcattt agccaactta aattaatgaa aaaatgttat
    19561 taatcgttga gctaaagtca ttagagatgc tttgtcctta atgtaaccat atcgcaataa
    19621 gttatgtttt taaattgagg gcattattat gaaaaaaata ccctgcgtga tgatgcgagg
    19681 tggaacctcg aggggcgcgt tcctgttagc ggaacattta cccgaagatc aaacgcagcg
    19741 cgataaaata ttgatggcaa ttatgggttc cggtaacgat ctggaaattg acggtattgg
    19801 cggcggtaat ccgctgacta gtaaagtcgc cattattagc cgttccagcg atccgcgtgc
    19861 tgatgttgat tatctgtttg ctcaagttat cgtccatgag caacgtgtcg ataccacgcc
    19921 taactgcggc aatatgctgt ctggcgttgg cgcattcgcc attgaaaatg gtttgattgc
    19981 agcgacttcg ccagttaccc gtgtacgtat ccgcaacgtc aatacgggta cgttcatcga
    20041 agctgatgtg caaacgccaa atggtgttgt cgagtacgag ggtagcgcca gaattgacgg
    20101 cgtaccgggt actgccgcac cggttgcgct cactttcctg aatgccgctg gaaccaaaac
    20161 cggaaaagtt ttcccgactg ataatcagat tgattatttt gacgatgtcc cggtgacctg
    20221 tatcgatatg gcgatgccag tcgtcattat tccggctgaa tatctgggaa aaacaggcta
    20281 tgaattaccg gcggaactgg atgccgacaa agcattatta gcccgcattg aatctatccg
    20341 tctacaagcg ggtaaagcaa tgggcttagg tgatgtcagt aatatggtta tccctaaacc
    20401 tgtgcttatt tctccagcgc agaaaggcgg ggcaattaat gtgcgttatt ttatgccgca
    20461 ttcttgccat cgcgcgttgg cgataaccgg tgctattgct atttccagta gttgtgcatt
    20521 ggaaggcacc gtcacccgac aaatcatccc ttctgtagga tatggcaata tcaatattga
    20581 acaccccagt ggtgcgctcg acgttcattt aactaatgaa ggtcaggatg ccacgacgtt
    20641 acgcgcatct gttattcgga cgaccagaaa aatattttcc ggtgaagttt atcttccctg
    20701 aaaagattcg ttgtcaggat aaggacaatc aataaaggac ttctgtatga gtcatacaga
    20761 aagaacagga ttttaaatga ataagaaatc gttatggaag ctaattctca tattagcgat
    20821 cccatgtatt attggcttta tgccagctcc ggcaggatta agcgaactgg cgtgggtgct
    20881 ttttggtatt tacctggcgg ccattgtggg gctggttatc aagcctttcc cggaacctgt
    20941 cgtactgtta attgccgttg ctgcctccat ggtggtggtc ggtaacttat ccgacggtgc
    21001 gtttaaaacc accgccgtat taagcggtta ctcttcaggt actacctggc tggtgttctc
    21061 ggcgtttacc ttaagcgccg catttgtgac caccggttta ggtaaacgta ttgcctatct
    21121 gctgattggt aaaatcggta acaccacgct gggtctgggt tacgttacgg tattcctcga
    21181 tctggtactg gctccggcaa caccgtctaa caccgcgcgt gcgggcggca ttgtgttacc
    21241 gatcatcaac agcgtggcgg tggctttggg gtccgaaccg gaaaaaagtc cgcgtcgtgt
    21301 cggacattac ctgatgatgt ccatttacat ggtcaccaaa accaccagct atatgttctt
    21361 taccgcaatg gcggggaaca ttctggcgct gaaaatgatc aacgacattc tgcacctgca
    21421 aattagctgg ggtggatggg cgctggccgc cggattgccg ggcatcatta tgctgctggt
    21481 caccccgctg gtgatttaca ccatgtatcc accagaaatt aagaaggtgg ataacaaaac
    21541 catcgctaaa gcgggccttg ccgaactggg accgatgaaa atccgcgaaa aaatgctgct
    21601 cggtgtcttt gtgctggcgc tgctgggctg gattttcagt aagtctctgg gggttgatga
    21661 atccaccgtg gcaatcgttg ttatggcgac tatgctgctg ctgggtatcg ttacctggga
    21721 agacgtggtt aaaaataaag gcggctggaa taccttaatc tggtacggcg gtattatcgg
    21781 cttaagctcc ttattatcga aagttaaatt tttcgaatgg ttagctgaag tctttaaaaa
    21841 taacctggca tttgatggtc acggtaacgt tgctttcttc gttattattt tcctcagcat
    21901 catcgtgcgt tatttcttcg cttccggtag tgcctatatc gttgctatgt taccggtatt
    21961 tgccatgctg gcgaacgtct ccggcgcacc gttaatgtta accgcgctgg cactgttgtt
    22021 ctccaactcc tatggcggca tggttactca ctatggcggc gcggcaggtc cggtcatctt
    22081 tggcgtgggt tataacgata ttaaatcctg gtggttggtc ggtgcggtac tgacgatatt
    22141 aaccttcctg gtgcatatca ccctcggagt gtggtggtgg aatatgctga tcggctggaa
    22201 catgctgtaa atatactcgt catacttcaa gttgcatgtg ctgagtctgc gttcgctcac
    22261 cccagtcact tacttatgta agctcctggg gattcactcg cttgtcgcct tcctgcaact
    22321 cgaattattt agagtatatc catttattat ctttttgcgc acttcacggt gcgcagatat
    22381 ctggagcatt taatgatcaa gttatctgaa aaaggcgtgt ttctcgccag taataacgaa
    22441 ataattgccg aagaacattt caccggcgaa attaaaaaag aagaagccaa aaaaggcact
    22501 attgcctggt ctattctctc ttctcataat acgtccggaa atatggataa acttaaaatt
    22561 aagtttgatt cattagcctc tcacgatatt acctttgttg gtatagtaca gaccgctaaa
    22621 gcgtccggta tggaacgttt cccgctgccg tatgtgctga ccaactgcca taactcactt
    22681 tgcgccgtcg gcggcaccat taacggtgat gaccatgttt ttggtttatc ggcggctcag
    22741 cgttatggcg gtatttttgt gcctccgcat attgcggtca tccatcaata tatgcgtgag
    22801 atgatggcag gtggcggcaa aatgatcctc gggtcagaca gtcacacccg ttacggtgca
    22861 ttagggacaa tggcagtcgg tgagggcggc ggcgagttgg taaaacaatt gcttaatgac
    22921 acctgggata tcgactatcc gggagttgtt gcggtgcatc tgaccggaaa accagcgccg
    22981 tatgtggggc cgcaggatgt ggcgctggct atcatcggtg ccgtgttcaa aaacggctac
    23041 gtcaaaaaca aagtgatgga attcgtaggt cccggtattg ctgcgctctc taccgatttc
    23101 cgtaacagtg ttgacgttat gaccactgaa acgacctgtt taagttctgt ctggcaaacc
    23161 gatgaagaag tgcataactg gctggcgctg cacggtcgcg gccaggatta ctgccagctt
    23221 aaccctcaac cgatggcgta ctacgatggc tgcatcagcg ttgatttaag cgccatcaaa
    23281 ccaatgattg cgctgccgtt ccacccgagc aacgtgtatg aaatcgacac gctgaaccag
    23341 aacctgaccg acattctacg tgagattgaa attgagtccg agcgcgtggc gcacggtaaa
    23401 gccaaactct cgctgctgga taaagtggaa aatggtcgcc tgaaagtgca gcaggggatt
    23461 atcgcgggct gttctggcgg taactacgaa aacgtcatcg cggcggcgaa tgcactgcgc
    23521 ggtcaatcct gtggcaatga caccttctcg ctggcggttt acccgtcatc acagccggtg
    23581 tttatggatc tcgccaaaaa aggtgtggta gcagatttga ttggcgcagg cgcaatcatc
    23641 agaaccgcgt tctgcggccc atgctttggc gcgggcgata cgccaatcaa caacggttta
    23701 agtattcgcc acaccacgcg taacttcccg aaccgcgaag gctctaagcc agctaatggg
    23761 cagatgtcag cggtggcgtt gatggacgct cgttctatcg ctgcgactgc gggaaacggt
    23821 ggctatttaa cttctgccag cgaactcgat tgctgggaca acgtgccgga gtacgccttc
    23881 gatgtaacgc cgtataaaaa ccgtgtttat cagggctttg tgaaaggggc aactcagcaa
    23941 ccgctgattt acggaccgaa cattaaagac tggccggaat taggtgcgct gactgacaat
    24001 atcgtcctca aagtgtgctc gaagatcctc gacgaagtga ccaccaccga cgaactgatc
    24061 ccttccggtg aaacctcttc ttatcgttca aatccgattg gtctggcgga gtttaccctg
    24121 tctcgccgcg atcccggtta tgttggcaga agtaaagcga ctgctgagct ggaaaatcag
    24181 cgtctggcgg ggaatgtcag cgagctgaca gaggtgtttg cgcgcattaa gcagattgct
    24241 ggtcaggagc atattgatcc gctgcaaact gaaattggca gcatggtcta tgcggtgaaa
    24301 ccaggcgatg gttctgcgcg tgaacaggcg gcgagctgcc agcgtgtgat tggcggtctg
    24361 gcgaatattg ccgaggagta cgcgactaaa cgctatcgtt ctaacgtcat caactggggg
    24421 atgttaccgc tgcaaatggc ggaagtgcca acctttgaag tgggggatta catttacatc
    24481 cctggcatta aagcggcgct ggataatccg ggtacgacgt ttaaaggtta tgtgatccat
    24541 gaagatgcgc cggtaacgga aattacgctc tatatggaaa gtctgactgc tgaagagcgc
    24601 gagattatca aggcgggtag tttgattaac ttcaataaaa accgtcagat gtaaaaagcg
    24661 ccatgtgaat gtaggtcgca ttcggcactt attgtcggat gcgatgctgg cgcatcttat
    24721 ccgacctaca aatcgcatcg aaccgtaggc cggataaggc gtttacgccg catccggcaa
    24781 atagttaata gctcttactt cttcgcctct gcaaccactt tgctacccac gccgcggtta
    24841 ttgtattccc acatgcggtt gtagttagtg tcattcagat tgcgctgtat ttcgtcgcta
    24901 tcgtcaacgc tgccggtatt acccgcaaac ggacgattag agatcaccgc atcgccccac
    24961 ggtttaaccg tgttaaaacc ttcgttgatg gcgctatcac ggatcaccac ctgaccgttg
    25021 gtattggcat caacatccag cgagcggccc agttgcgcca cgccatcacc ggaagcattg
    25081 aaacggctgt ttacggcgag gaaaccgtaa tagatgttgg acagcgtagc cggtgcaaac
    25141 acatacgctt cttgctgagt acgtgagttc accacgcgga attcggtgtt atcgaacacc
    25201 actgcgccgc gaccagaaac gatatccaca tccccttcaa tgtagctgtt ggtcaccagc
    25261 gtacgcggct gacgattcgt ttccagacgg ttctgcacac cgctgttggt gacaaagaag
    25321 gtgttctgac gaccgagaat gttaacgttg ttaatctgta cctggtcacc gtcagtacgc
    25381 agtgccaccg ccggatggtt acctgcatct acgctatcgc ccagcgtgtt ttcgatggtc
    25441 agattttgca gttgcaggcc attgttttgt gaccagaaga ccgcagaaca cagaacaccg
    25501 atactgtcgc tgcgtttgcg ctggcagcta tcgtacatat accacgctgg tttacctggc
    25561 atatatttgc cgcgcgggtt gacgtcgtga cgccagtcgg cagggctcat gcccccatca
    25621 agggaaagcc caatcttcac atcaatcggt ttttcacctg taccgtacag agtaattcca
    25681 cctggagcag cagggacata caccgttccc tgatactcac caggcatcac ggcaatatac
    25741 tggcgcttgt tggtgcgctt gataattgcc gcatctaccg ccgcctgaat cgtggtatgc
    25801 gttacacctt gagtacccgc cgggccgaca acaaagtcag gttgcgcagg cagggttatc
    25861 ggggacggat tccacgctgc agcacctggt gtcagggatg cataatagtg ttgagcatcg
    25921 aaattctgcg cttcttttgc cgacagaatc gggcgagaag aggtaccagg cgcagtttga
    25981 tcagaaggac gttgatcggg tggtgttgag ctacaggcgg tcagcgtcac gccaaaagcc
    26041 aatgccagcg ccagacggga aactgaaaat gtgttcacag gttgctccgg gctatgaaat
    26101 agaaaaatga atccgttgaa gcctgctttt ttatactaac ttgagcgaaa cgggaaggta
    26161 aaaagacaaa aagttgtttt taataccttt aagtgatacc agatagcatt gcgccatctg
    26221 gcagagtgat taactaaaca tcgcggtaat cgatgcgctt gccagagagt ggaaatgaac
    26281 gttaaacccg accatcgcgc cgctggctcc ttcatcgaca tcaatacgct ctacatccag
    26341 cgcgtgaacg gtaaaaatgt agcgatgggt ttcgcctttc ggcggcgctg cgccatcgta
    26401 tccggtttta ccaaaatcgg tacgcgtctg caaaacgccg tctggcattg ctaccagacc
    26461 agagccaaac ccttgcggta atacgcgggt atcagcgggt aaattaacaa ctacccagtg
    26521 ccaccagccg gagccggttg gcgcatccgg gtcgtagcag gtgacaacaa aacttttcgt
    26581 tcccgcagga acatcatccc acgccagatg cggtgaaata ttatcgccat cgtaacccat
    26641 gccgttaaag acatgacgat gcggcaactt atcgccatcg cgcagatcgt tactgatgag
    26701 tttcataaac cctcctttct tgtttgcaga aagtgtagcc agaaacccac acgctgactt
    26761 cccgttattg gcaaaaaaat gtttcatcct gcaccgcacg attaaccgct gcggtcagac
    26821 gctgcaactg ttgcgggtga ataatatagg gcggcatcag gtaaatcagt ttgccaaaag
    26881 gccggatcca gacaccctgt tcgacaaaga atttttgcag cgccgccata ttcaccggac
    26941 gagttgtttc gactacgccg attgccccca gcacgcgcac atcggcaacc atttcggcat
    27001 cacgggcggg ggcaagttgc tcgcgcagct gtacttcaat agccgccacc tgttgctgcc
    27061 agtcgccaga ttcgagaatc gccaggctgg cgtttgcagc cgcacaggcc agcggattgc
    27121 ccataaaagt tggcccgtgc ataaagcagc cggcttcgcc gttactgatg gtttctgcaa
    27181 cctcgcgcgt ggtgagtgtg gcggaaaggg tcattgtgcc gccggttaag gctttaccga
    27241 ggcacaaaat gtccggcgcg atttctgcat gttcacaggc aaacagtttg ccggtacgac
    27301 caaatccggt ggcgatctcg tcggcaatca gcaagatacc ttcgcgatcg cacattttgc
    27361 ggattcgttt taaccattcc ggatggtaca tgcgcatccc gcctgcgccc tggacaatcg
    27421 gttcaatgat caccgccgcg atttcatgac gatgcgccgc catcaggcgg gcaaagccca
    27481 ccatatcgcg ctcatcccat tcaccatcca tgcggctttg cggggcggga gcaaacaagt
    27541 tttctggcag atagcctttc cacaggctgt gcattgagtt gtccggatcg cacaccgaca
    27601 tcgcgccaaa ggtatcgcca tgataaccat tgcggaaggt cagaaaacgc tggcgcgctt
    27661 cgcctttggc ctgccagtac tgcaacgcca ttttcatcgc cacttccacc gctacggagc
    27721 cggagtccgc gagaaaaacg cattccagcg gttgcggcgt catcgccacc agtttgcggc
    27781 acagctcaat ggctggcgca tgagtgatac cgccaaacat cacatgcgac atggcatcaa
    27841 tttgcgtctt catcgccgca ttaagctgcg ggtgattgta gccgtggatc gccgcccacc
    27901 aggacgacat accgtcaacc aggcgtctgc catcagacaa aattagctcg caaccttcgg
    27961 cgcttgccac cggataaacc ggtaacgggg aggtcatgga tgtgtatggg tgccagatat
    28021 ggcgttggtc aaaggcaaga tcgtccgttg tcataatcga cttgtaaacc aaattgaaaa
    28081 gatttaggtt tacaagtcta caccgaatta acaacaaaaa acacgttttg gagaagcccc
    28141 atggctcacc gcccacgctg gacattgtcg caagtcacag aattatttga aaaaccgttg
    28201 ctggatctgc tgtttgaagc gcagcaggtg catcgccagc atttcgatcc tcgtcaggtg
    28261 caggtcagca cgttgctgtc gattaagacc ggagcttgtc cggaagattg caaatactgc
    28321 ccgcaaagct cgcgctacaa aaccgggctg gaagccgaga ggttgatgga agttgaacag
    28381 gtgctggagt cggcgcgcaa agcgaaagcg gcaggatcga cgcgtttctg tatgggcgcg
    28441 gcgtggaaga atccccacga acgcgatatg ccgtacctgg aacaaatggt gcagggggta
    28501 aaagcgatgg ggctggaggc gtgtatgacg ctgggcacgt tgagtgaatc tcaggcgcag
    28561 cgccttgcga acgccgggct ggattactac aaccataacc tcgacacctc gccggagttt
    28621 tacggcaata tcatcaccac acgtacttat caggaacgcc ttgataccct ggaaaaagtg
    28681 cgcgatgccg ggatcaaagt ctgctcaggt ggcattgtgg gtttaggcga aacggtaaaa
    28741 gatcgcgccg gattattact gcaactggca aacctgccga cgccgccgga aagcgtgcca
    28801 atcaacatgc tggtgaaggt gaaaggcacg ccgcttgccg ataacgatga tgtcgatgcc
    28861 tttgatttta ttcgcaccat tgccgtagcg cggatcatga tgccgacctc ttacgtacgc
    28921 ctttctgccg gacgcgagca gatgaacgaa cagactcagg cgatgtgctt tatggcaggc
    28981 gcaaactcga ttttctacgg ttgcaaactg ctaaccacgc cgaatccgga agaagataaa
    29041 gacctgcaac tgtttcgcaa actggggcta aatccgcagc aaactgccgt gctggcaggg
    29101 gataacgaac aacagcagcg tctggagcag gcactgatgg ccccggacac tgacgaatat
    29161 tacaacgcgg cagcactatg agctggcagg agaaaatcaa cgcggcgctc gatgcgcggc
    29221 gtgctgccga tgccctgcgt cgccgttatc cagtggcgca aggagccgga cgctggctgg
    29281 tggcggatga tcgccagtat ctgaactttt ccagtaacga ttatttaggt ttaagccatc
    29341 atccgcaaat tatccgtgcc tggaagcagg gggcggagca atttggcgtc ggtagcggcg
    29401 gttccggtca cgtcagcggt tatagcgtgg cgcatcaggc gctggaagaa gaactggccg
    29461 agtggctggg ctattcgcgg gcactgctgt ttatctctgg ttttgccgct aatcaggcgg
    29521 tcattgccgc gatgatggcg aaagaggacc gtattgttgc cgaccggctt agccatgcct
    29581 cattgctgga ggctgccagt ttaagcccgt cgcagcttcg ccgttttatc cataacgatg
    29641 tcgctcatct ggcgcgactg cttgcttccc cctgtccggg gcagcaactg gtggtgacag
    29701 aaggcgtgtt cagcatggac ggcgatagtg cgccactggc ggaaatccag caggtaacgc
    29761 aacagcacaa tggctggttg atggtcgatg atgcccacgg cacgggcgtt atcggggagc
    29821 aggggcgcgg cacctgctgg ttgcaaaagg taaaaccaga attgctggta gtgacttttg
    29881 gcaaaggatt tggcgtcagt ggggctgcgg tgctttgctc cagtacggtg gcggattatc
    29941 tgctgcaatt cgcccgccat cttatctaca gcaccagtat gccgcccgct caggcgcagg
    30001 cattacgtgc gtcactggcg gtcattcgca gtgatgaggg tgatgcacgg cgcgaaaaac
    30061 tggcggcact cattacgcgt tttcgtgccg gagtacagga tttgccgttt acgcttgctg
    30121 attcatgcag cgccatccag ccattgatcg tcggtgataa cagccgtgcg ttacaactgg
    30181 cagaaaaact gcgccagcaa ggctgctggg tcacggcgat tcgcccgcca accgtacccg
    30241 ctggtactgc gcgactgcgc ttaacactaa cctccgcgca tgaaatgcag gatatcgacc
    30301 gtctgctgga ggtgctgcat ggcaacggtt aataaacaag ccattgcagc ggcatttggt
    30361 cgggcagccg cgcactatga gcaacacgca gagttacagc gccagagtgc tgacgtctta
    30421 ctggcaatgc tcccacagcg taaatacacc cacgtactgg acgcggggtg tgggccaggc
    30481 tggatgagcc gccactggcg agagcgtcac gcgcaggtga cagcgttaga tctctcgccg
    30541 ccaatgcttg cgcaagcccg ccataaagat gccgcagatc attatctggc gggagatatc
    30601 gaatccctgc cgttagcgac tgcgacgttc gatcttgtat ggagcaatct cgcggtgcag
    30661 tggtgcggta atttatcaac ggcactccgc gagctgtatc gggtggtgcg ccccggaggc
    30721 gtggtcgcgt ttaccacgct ggtgcaggga tcgttacccg aactgcatca ggcgtggcag
    30781 gcggtggacg agcgcccgca tgctaatcgc tttttaccgc cagatgaaat cgaacagtcg
    30841 ctgaacggcg tgcattatca acatcatatt cagcccatca cactatggtt tgatgatgcg
    30901 ctcagtgcca tgcgttcgct gaaaggcatc ggtgccacgc atcttcatga agggcgcgac
    30961 cagcgaatat taacgcgttc acagttgcag cgattgcaac tggcctggcc gcaacaacag
    31021 gggcgatatc ctctgacgta tcatcttttt ttgggagtga ttgttcgtga gtaaacgtta
    31081 ttttgtcacc ggaacggata ccgaagtggg gaaaactgtc gccagttgtg cacttttaca
    31141 agccgcaaag gcagcaggct atcggacggc aggttataaa ccggtcgcct ctggcagcga
    31201 aaagaccccg gagggtttac gtaatagtga cgcgctggcg ttacagcgca acagcagcct
    31261 gcagctggat tacgaaacgg taaatcctta caccttcgca gaacccacct caccgcacat
    31321 catcagcgcg caagagggca ggccgataga atcatcggta atgagctccg gattacgcgc
    31381 gcttgaacaa caggctgact gggtgttagt ggaaggcgct ggcggctggt ttacgccgct
    31441 ttctgacact ttcacttttg cggattgggt aacacaggaa caactgccgg taatactggt
    31501 ggttggcgtg aaacttggct gtattaatca cgcgatgttg acggcacagg cgatacaaca
    31561 cgccgggctg actctggcgg gttgggtggc gaacgatgtc acgcctccgg gaaaacgtca
    31621 cgctgaatat atgaccacgc tcacccgcat gattcccgcg ccgctgctgg gagagatccc
    31681 ctggcttgca gaaaatccag aaaatgcggc aaccggaaag tacataaacc ttgccttgtt
    31741 gtagccatcc tgtatttggt taaattgcga gcgagatcgc gtcttcgatt gactgcaatt
    31801 taaccaatta aattctaaaa taatcacgaa aaaaatttta cttccgcatc atgcggcgaa
    31861 tgtgggaatt gcccagg
//
LOCUS       DAPGOF010000043        31672 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.043, whole genome shotgun sequence.
ACCESSION   DAPGOF010000043 DAPGOF010000000
VERSION     DAPGOF010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31672
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.043"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..4483
                     /gene="sslE"
                     /locus_tag="RHV13_001687"
     CDS             <1..4483
                     /gene="sslE"
                     /locus_tag="RHV13_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
                     /protein_id="HDV1241230.1"
                     /translation="GGSGSSSDTPPVDSGTGSLPEVKPDPTPNPEPTPEPTPDPEPTP
                     EPTPDPEPTPEPEPEPVPTKTGYLTLGGSQRVTGATCNGESSDGFTFKPGEDVTCVAG
                     NTTIATFNTQSEAARSLRAVEKVSFSLEDAQELAASDNKKSNAVSLVTSSNSCPADTE
                     QVCLTFSSVIESKRFDSLYKQIDLAPEEFKKLVNEEVENNAATDKAPSTHTSPVVPAT
                     TPGTKPDLNASFVSANAEQFYQYQPTEIIRSEGRLVDSMGNGVVGVNYYTSSGRGVTG
                     ENGKFNFSWGETISFGIDTFELGSVRGNKSTIALTELGDEVRGANIDQLIHRYSQAGK
                     NDEREVPDVVRQVFAEYPNVINEIINLSLSNGEALSEGDQTFERTNEFLEQFESGQAK
                     EIDTAICDSLGGCNSQRWFSLTARNVNEGQIQGVINKLWGVDKDYKSVTKFHVFHDST
                     NFYGSTGNARGQAVVNISNAAFPILMARNDKNYWLAFGEKRAWDKNELAYITEAPSIV
                     QPENVTRETATFNLPFISLGQVGKGKLMVIGNPHYNSILRCPNGYSWNGSVNKDGQCT
                     LNSDSDDMKNFMENVLRYLSNDRWLPDAKSNMTVGTNLDTVYFKKHGQVTGNSAAFGF
                     HPDFAGISVEHLSSYGDLDPQDMPLLILNGFEYVTQVGGDPYAVPLRADTSKPKLTQQ
                     DVTDLIAYLNKGGSVLIMENVMSNLKEESASGFVRLLDAAGLSMALNKSVVNNDPQGY
                     PDRVRQQRATGIWVYERYPFVDGKPPYTIDETTKEVIWKYQQDNKPDDKPKLEVASWQ
                     EEVEGKQVTRYAFIDEAEFKTKESLEAAKAKIFEKFPGLQECKDSTYHYEINCLEYRP
                     GTKVPVTGGMYVPQYTQLNLSADTAKAMVQAADLGTNIQRLYQHELYFRTNGRKGERL
                     SSVDLERLYQNMSVWLWNKIEYRYENDKDDELGFKTFTEFLNCYANDAYAGGTKCSAD
                     LKKSLVDNKMIYGESSGKAGMMNPSYPLNYMEKPLTRLMLGRSWWDLNIKVDVEKYPG
                     AVSAEGEKVTESISLYSNPTKWFAGNMQSTGLWAPAQKEVTIESKANVPVTVTVALAD
                     DLTGREKHEVALNRPPRVTKTYSLDASGTVTFKVPYGGLIYIKSDSKEEQSAEFTFTG
                     VVKAPFYKDGAWKNAPNSPAPLGELESDAFVYTTPKKNLEASNYKGGLKQFAEELDTF
                     ASSMNDFYGRDGENGNHRMFTYKALTGHKHRFTNDVQISIGDAHSGYPVMNSSFSTNS
                     TTLPTTPLNDWLIWHEVGHNAAETPLTVPGATEVANNVLALYMQDRYLGKMNRVADDI
                     TVAPEYLEESNGQAWARGGAGDRLLMYAQLKEWAEKNFDIKKWYPEGSLPAFYSEREG
                     MKGWNLFQLMHRKARGDEVGKTKFGGRNYCAESNGNAADTLMLCASWVAQTDLSEFFK
                     KWNPGANAYQLPGVSEMNFEGGVSQSAYETLAALNLPKPQQGPETINQVTEHKMSVE"
     gene            4810..5619
                     /gene="pppA"
                     /locus_tag="RHV13_001688"
     CDS             4810..5619
                     /gene="pppA"
                     /locus_tag="RHV13_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase PppA"
                     /protein_id="HDV1241231.1"
                     /translation="MLFDVFQQYPAAMPILATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGEMPSAQSKISLALPRSHCPHCQQTIRVRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWGLAVMILSAWLIAASVIDLDHQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGGW
                     VGALSLPNVALIASCCGLIYAVITKRGSTTLPFGPCLSLGGIATLYLQALF"
     gene            5685..6095
                     /gene="gspS2"
                     /locus_tag="RHV13_001689"
     CDS             5685..6095
                     /gene="gspS2"
                     /locus_tag="RHV13_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HDV1241232.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCASHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGTTVKMTIISEAGAQTTQTPDAFLTSYQRQMCADPTVKLM
                     ITEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            6113..7072
                     /gene="gspC"
                     /locus_tag="RHV13_001690"
     CDS             6113..7072
                     /gene="gspC"
                     /locus_tag="RHV13_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HDV1241233.1"
                     /translation="MARVVFRDARIYLIQWLTKIRHTLSQRQSLNTDQEHLRKIARGM
                     FWLMLLIISAKMAYSLWRYFSFSAEYTAVSQSVNKPTRADAKAFDKNDVQLISQQNWF
                     GKYHPVAAPVKQPEPAPVAETRLNVVLRGIAFGARPGAVIEEGGKQQVYLQGETLGSH
                     NAVIEEINRDHVMLRYQGKIERLSLAEEERSTVAVTRQKAISDEAKQAVAEPAVSAPV
                     EIPTAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFKEGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARHDISIALR"
     gene            7102..9162
                     /gene="gspD"
                     /locus_tag="RHV13_001691"
     CDS             7102..9162
                     /gene="gspD"
                     /locus_tag="RHV13_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HDV1241234.1"
                     /translation="MFWRDITLSVWRKKTTCLKTKKRLLPLVLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREVVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWASKDAGLM
                     QFANGTQIPIGTLGAAISQAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDEQGLSLMPHTAQPVLPAQNQALPPEVRAFLNA
                     GRTR"
     gene            9162..10655
                     /gene="gspE"
                     /locus_tag="RHV13_001692"
     CDS             9162..10655
                     /gene="gspE"
                     /locus_tag="RHV13_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HDV1241235.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSQQLTAQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCP
                     DCRQAAPATDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            10655..11878
                     /gene="gspF"
                     /locus_tag="RHV13_001693"
     CDS             10655..11878
                     /gene="gspF"
                     /locus_tag="RHV13_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HDV1241236.1"
                     /translation="MALFYYQALERNGRKNKGMIEADSARHARQLLRAKELIPVNIEA
                     RMNASSGGILQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPASTRTLIAM
                     SDALQASGVYWLAGLLGLLVLGQRLLKNPAMRLRWDKTLLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            11895..12350
                     /gene="gspG"
                     /locus_tag="RHV13_001694"
     CDS             11895..12350
                     /gene="gspG"
                     /locus_tag="RHV13_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HDV1241237.1"
                     /translation="MNSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            12354..12917
                     /gene="gspH"
                     /locus_tag="RHV13_001695"
     CDS             12354..12917
                     /gene="gspH"
                     /locus_tag="RHV13_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HDV1241238.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATDSESPAKKAAQDF
                     LTRFAQFKDRAVIEGQTLGVLIDPPGYQFMQRRQRQWLPVSATRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYS
                     AAQNACWAVKLAHDGALSLSQCDERMP"
     gene            12914..13285
                     /gene="gspI"
                     /locus_tag="RHV13_001696"
     CDS             12914..13285
                     /gene="gspI"
                     /locus_tag="RHV13_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HDV1241239.1"
                     /translation="MKRGFTLLEVMLALAIFALAATAVLQIASGALSNQHVLEEKTVA
                     GWVAENQTALLYLMTREQRAVRHQGESDMAGSRWYWRTTPQNTGNALLQAVDIEVSLH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            13282..13887
                     /gene="gspJ"
                     /locus_tag="RHV13_001697"
     CDS             13282..13887
                     /gene="gspJ"
                     /locus_tag="RHV13_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HDV1241240.1"
                     /translation="MRRTCAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVAGH
                     DQKLNLMQQTMSFLTHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHDGYLERLAWPLTDAAGSVKPTMQKLIPADSLRLQFYDG
                     TRWQENWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQLS"
     gene            13884..14861
                     /gene="gspK"
                     /locus_tag="RHV13_001698"
     CDS             13884..14861
                     /gene="gspK"
                     /locus_tag="RHV13_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HDV1241241.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNVLAQPTTASRPLALQQLIALISRLNVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMTRQQININ
                     TLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            14858..16036
                     /gene="gspL"
                     /locus_tag="RHV13_001699"
     CDS             14858..16036
                     /gene="gspL"
                     /locus_tag="RHV13_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HDV1241242.1"
                     /translation="MSSMLEIFFPLCASDTIRWQRRTPDVEHGIWPDVADERLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVARKQIPTILPALLEEELNQDIDELHFALLKIDQQQ
                     ATVAVIHQQHMHNIAQWLQENGITRATVAPDWMSIPCGYMAGDAQRVICRINECRGWS
                     AGWALAPVMFRAQLNEQDLPISLTVVGIAPEELSAWAGADAERLTVTALPAITTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EKQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLSDIEM
                     RGFTFDQKRQTLHLQLRAANFASFDKLRSALATDYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            16038..16574
                     /gene="yghD"
                     /locus_tag="RHV13_001700"
     CDS             16038..16574
                     /gene="yghD"
                     /locus_tag="RHV13_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HDV1241243.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLAQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLFWQPLSERIEQQETMLQQLVAMNTRLKRAAPDIIAARKSATTAPAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIEPVVFNDLLKWLNALDEKYALRVTQIDVS
                     AAEKPGMVNVQRLEFGRG"
     gene            complement(16723..16798)
                     /locus_tag="RHV13_001701"
     tRNA            complement(16723..16798)
                     /locus_tag="RHV13_001701"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16763..16765),aa:Phe,seq:gaa)
     gene            complement(16904..17611)
                     /locus_tag="RHV13_001702"
     CDS             complement(16904..17611)
                     /locus_tag="RHV13_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDV1241244.1"
                     /translation="MVIGPFINASAILLGGMLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNFVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWLA"
     gene            18009..20144
                     /gene="speC"
                     /locus_tag="RHV13_001703"
     CDS             18009..20144
                     /gene="speC"
                     /locus_tag="RHV13_001703"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HDV1241245.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGIDAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLVE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGDVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(20194..21450)
                     /gene="nupG"
                     /locus_tag="RHV13_001704"
     CDS             complement(20194..21450)
                     /gene="nupG"
                     /locus_tag="RHV13_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HDV1241246.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(21652..22731)
                     /gene="mltC"
                     /locus_tag="RHV13_001705"
     CDS             complement(21652..22731)
                     /gene="mltC"
                     /locus_tag="RHV13_001705"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HDV1241247.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(22796..23071)
                     /locus_tag="RHV13_001706"
     CDS             complement(22796..23071)
                     /locus_tag="RHV13_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HDV1241248.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(23099..24151)
                     /gene="mutY"
                     /locus_tag="RHV13_001707"
     CDS             complement(23099..24151)
                     /gene="mutY"
                     /locus_tag="RHV13_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDV1241249.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYLLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            24312..25031
                     /gene="trmB"
                     /locus_tag="RHV13_001708"
     CDS             24312..25031
                     /gene="trmB"
                     /locus_tag="RHV13_001708"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDV1241250.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            25031..25357
                     /locus_tag="RHV13_001709"
     CDS             25031..25357
                     /locus_tag="RHV13_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HDV1241251.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            25541..26260
                     /locus_tag="RHV13_001710"
     CDS             25541..26260
                     /locus_tag="RHV13_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HDV1241252.1"
                     /translation="MMRKMLLAAALSVTAITAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            26436..27482
                     /gene="ansB"
                     /locus_tag="RHV13_001711"
     CDS             26436..27482
                     /gene="ansB"
                     /locus_tag="RHV13_001711"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HDV1241253.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKTVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            27599..28606
                     /locus_tag="RHV13_001712"
     CDS             27599..28606
                     /locus_tag="RHV13_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HDV1241254.1"
                     /translation="MKKQWIVGTALLMLMTGNVWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKELDKKMAAAQKAADAYWGKDANGKQMTREDAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(28662..29963)
                     /locus_tag="RHV13_001713"
     CDS             complement(28662..29963)
                     /locus_tag="RHV13_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HDV1241255.1"
                     /translation="MDSYIAITLFGCFFVLVFIGMPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMIGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKQGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQSCKA
                     FLDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILE
                     SVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGI
                     FMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISG
                     VKIQHLIKPLLPFYAALLVALMMITYIPQISLFIPQLLGLM"
     gene            complement(29963..30466)
                     /locus_tag="RHV13_001714"
     CDS             complement(29963..30466)
                     /locus_tag="RHV13_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDV1241256.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGLVMMFYALCFINQSIQQLK
                     QHAQEAS"
     gene            complement(30511..31497)
                     /locus_tag="RHV13_001715"
     CDS             complement(30511..31497)
                     /locus_tag="RHV13_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDV1241257.1"
                     /translation="MKMRSFTRSLVCASLLALVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQMSKGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKDQAHFNKVVFGEVGKEIMDSTKDKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQQQILETAAKKSEAYQQKLWEKI
                     DADTRAQAKAMGGEIVKVDKAPFRAAVQPLFDDFKKDPKQAALLEKFDNAAQ"
BASE COUNT         7990 a         7793 c         8580 g         7309 t
ORIGIN      
        1 cggtggttcc ggatcttcct ccgatacgcc gcctgtagat tctggaacag ggtctttgcc
       61 ggaagtgaaa cctgatccaa caccaaaccc ggagccgacg cctgagccaa cgccggaccc
      121 agaacctacg ccggaaccga cacctgatcc tgagccaaca ccagaaccgg agccagaacc
      181 tgttcctacg aaaacgggtt atctgaccct gggcggaagc cagcgggtaa ctggtgctac
      241 ctgtaatggt gaatccagcg atggctttac atttaaacct ggcgaggacg ttacttgcgt
      301 ggcgggtaac acgacaattg ccaccttcaa cactcagtca gaagctgcgc gtagcctgcg
      361 tgcggttgaa aaagtgtcgt ttagtcttga ggacgcgcaa gaactggcgg cttccgacaa
      421 taagaaaagc aatgcggttt cgctggtaac gtccagtaac agctgtccgg cggatacaga
      481 acaggtttgc ctgacgttct cctcggtgat agagagtaaa cgtttcgact cgctgtataa
      541 gcaaatcgat ctggcaccgg aagagttcaa aaagctggtc aatgaagagg tggaaaacaa
      601 tgccgcgacc gataaagcgc catccactca tacttcaccg gtcgtgcccg ccaccactcc
      661 gggaacaaaa ccggatctaa acgcttcctt cgtgtcggct aacgcggaac agttttatca
      721 gtatcaacct actgaaatta ttcgctccga aggccgactg gtagatagca tgggcaatgg
      781 tgtggttggc gtaaattact acaccagctc aggccgtggc gtaactggcg aaaacggcaa
      841 attcaatttc agctggggcg aaaccatctc ctttggtatc gacacctttg aactgggctc
      901 agtacgcggc aataagtcga ccattgcatt gactgagttg ggcgacgaag ttcgcggcgc
      961 gaatatcgat cagcttattc atcgttattc ccaggccgga aaaaatgatg agcgtgaagt
     1021 gccggacgta gtgcgccagg tctttgccga atatcccaac gtgatcaacg agattatcaa
     1081 tctctcgtta tccaacggcg aggcattgag cgaaggcgat caaacctttg agcggacaaa
     1141 cgaatttctt gagcagtttg aatccgggca ggctaaagag attgatacgg cgatttgtga
     1201 ctcccttggg ggctgcaact ctcagcgttg gttctcgttg acagcacgca atgttaacga
     1261 aggtcagatt cagggcgtta tcaacaagct gtggggtgtg gataaagatt acaaatcggt
     1321 gacgaaattc cacgtcttcc atgactctac caacttctat ggcagcactg gtaatgcgcg
     1381 cggtcaggca gtggtaaata tctccaacgc ggcattcccg attctgatgg cgcgtaatga
     1441 taaaaactac tggctggcct tcggcgaaaa acgcgcctgg gataaaaatg agctggcgta
     1501 cattactgaa gcgccttcca ttgtgcaacc agagaacgtg acacgcgaaa ctgcgacctt
     1561 caacctgccg tttatctcgc tggggcaagt cggcaaaggc aagctgatgg ttatcggtaa
     1621 tccgcactac aacagcattt tgcgttgccc gaacggttac agctggaacg ggagcgttaa
     1681 taaagacgga cagtgtacgc tcaacagcga ctcggatgac atgaagaact tcatggagaa
     1741 cgtgttgcgc tatctgtcaa atgatcgctg gttgccggat gcaaaatcca atatgaccgt
     1801 gggtactaac ctggacacgg tgtatttcaa aaaacacggg caggttacag gaaacagtgc
     1861 tgcgtttggc ttccatccgg attttgcggg tatctctgtt gagcatttaa gtagctatgg
     1921 cgatctcgat ccgcaggaca tgccactgct gatcctcaac ggctttgagt atgtgactca
     1981 ggtggggggc gatccctatg cagtgcctct gcgtgcagat accagcaaac cgaagctgac
     2041 ccagcaggat gtgaccgatc tgatcgccta tctgaacaaa ggtggctcgg tgctgatcat
     2101 ggaaaacgtg atgagcaatc ttaaggaaga gagcgcgtct ggctttgtgc gtctgctgga
     2161 tgccgcaggc ctgtcaatgg cactgaacaa gtcggtggtg aataacgatc cgcaggggta
     2221 tccggatcgc gtacgccaac aacgcgcaac gggtatttgg gtctatgaac gttatccgtt
     2281 tgttgatggt aaaccgccgt ataccattga tgaaacaacg aaagaagtta tctggaaata
     2341 ccagcaagac aacaagcctg atgataagcc gaaactggaa gttgccagct ggcaggagga
     2401 agttgagggc aaacaggtaa cgcgttatgc ctttattgat gaggcggagt ttaaaacaaa
     2461 agagtctctg gaggctgcaa aggcaaaaat ctttgagaag tttcctggat tacaggagtg
     2521 taaggactcg acatatcact acgaaatcaa ctgtctggaa taccgtcctg gtacgaaagt
     2581 tccggtaacc ggtggcatgt atgttccgca gtatacgcaa ctgaatctca gcgccgatac
     2641 tgcgaaagcg atggtgcagg cggcggattt aggcaccaat attcagcgtc tgtatcagca
     2701 cgagctttac ttccgtacca atggtcgcaa aggtgagcgt ctgagcagcg tcgatctgga
     2761 acgtctgtac cagaacatgt cggtctggct gtggaataaa attgaatatc gctatgaaaa
     2821 cgacaaggat gacgagctgg gctttaaaac gttcaccgag ttcctgaact gctacgccaa
     2881 tgatgcttat gcaggcggca ccaagtgttc tgcagatctc aaaaaatcgc tggtcgataa
     2941 caagatgatc tacggtgaaa gtagtggcaa agcgggcatg atgaacccga gctatccgct
     3001 caactatatg gaaaaaccgc tgacgcgcct gatgctgggg cgttcctggt gggatctgaa
     3061 catcaaagtt gatgtcgaga agtatccggg agcggtatcg gctgaaggtg agaaagttac
     3121 tgaaagcatc agcctgtact cgaacccgac caaatggttt gcgggtaaca tgcagtctac
     3181 tggcctgtgg gctccggctc agaaagaggt caccattgag tctaaggcga acgttcctgt
     3241 gactgtcacc gtggcgctgg ccgacgatct gaccggacgc gagaagcatg aagttgcgct
     3301 gaaccgtccg ccaagagtga ctaaaacata ctctctggat gctagcggca cggtgacgtt
     3361 caaggttcct tatggcggtc tgatttatat caagagcgac agtaaagagg agcaatcagc
     3421 tgaatttacc tttaccggtg tagtaaaagc gccgttctat aaagatggcg catggaaaaa
     3481 cgctccgaac tcccctgcgc cgctgggcga gctggaatca gacgcgttcg tctacaccac
     3541 gccgaagaag aaccttgagg ccagcaatta caagggcggt ctgaaacaat tcgctgagga
     3601 actggatacc tttgccagct cgatgaatga cttctatggc cgtgatggcg aaaacggtaa
     3661 ccaccggatg tttacctata aagcattgac gggacataaa catcgtttca caaacgatgt
     3721 gcagatctcc atcggtgatg cgcactcggg ttatccggta atgaacagca gcttctcgac
     3781 gaacagcacc acgctgccga cgacgccgct gaacgactgg ctgatctggc atgaagtcgg
     3841 tcataacgcc gcagaaacac cgctgactgt accgggtgca actgaagtcg cgaacaacgt
     3901 gctggcgctg tacatgcagg atcgttatct cggcaagatg aaccgtgtcg ctgacgatat
     3961 taccgtcgca ccggaatatc tggaggagag caacggtcag gcatgggcgc gcggcggtgc
     4021 gggtgaccgt ctgctgatgt acgcacaact gaaggaatgg gcagagaaaa actttgatat
     4081 caagaaatgg tatccagaag gctctctgcc agcgttctac agcgagcgtg aagggatgaa
     4141 aggctggaat ctgttccagt tgatgcatcg taaagcacgc ggcgatgagg ttggtaaaac
     4201 caagtttggc ggtagaaatt actgtgccga atccaacggt aacgctgccg acacgctgat
     4261 gctgtgtgcc tcctgggtcg cccagacgga tctttcggag ttctttaaga aatggaatcc
     4321 gggtgcgaat gcttaccagt tgccgggggt gagcgagatg aacttcgagg gcggtgtgag
     4381 ccagtcggct tacgagacgc tggcggcgct taatctgccg aaaccgcagc aagggccgga
     4441 aaccattaat caggttaccg agcataagat gtcagtcgag taagccagta ggccggataa
     4501 ggcgctcgcg ccgcatccgg catgatataa ggcgcaccgg gtcaacacat atgcccgatg
     4561 cgcaagctta tcgggcctac agagagggaa agctgcgttt atttgcgttg gcagacacct
     4621 gtaggccgga taaggcgctc gcgccgcatc cggcatgata taaggcgcat cgggttaaca
     4681 catatgcccg atgcgccatc ttatcgggtc ttcagggaaa gccgcgatta cttgcgttgg
     4741 cagataccag taggccggat aaggcgtttt acgccgcatc cggcaagtta caacaaacaa
     4801 cctttaacca tgctttttga tgtttttcag caataccccg cggcgatgcc catactggct
     4861 accgtcggag gattaatcat cggtagtttt ttgaatgtgg tgatttggcg ttaccccatc
     4921 atgctgcgcc aacaaatggc ggagtttcac ggtgaaatgc cgagtgcgca gtcaaaaata
     4981 agcctggcgc tgccacgctc gcactgtccg cattgccagc agactatccg cgttcgtgac
     5041 aatattccgc tgctctcctg gctgatgctc aaaggtcgct gccgcgattg tcaggcgaaa
     5101 atcagcaagc gttatccgct ggtggagtta ttgacagcac tcgctttttt gctggcgagt
     5161 ctggtctggc cagaaagtgg atgggggctg gcggtgatga tattatccgc ctggctgatt
     5221 gccgcgagcg tcatcgacct cgatcaccaa tggctgcccg atgttttcac tcagggcgta
     5281 ttgtggacag ggctgattgc ggcatgggcg caacagagtc cgctcacgct acaagatgca
     5341 gtcaccggcg tcctggtggg atttatcact ttttactcgc tgcgctggat agccggaata
     5401 gttctgcgta aagaagcatt aggcatgggc gatgtattat tgttcgctgc tttaggtggc
     5461 tgggtggggg cgttgtcgct gcccaatgtt gctttaatcg catcatgctg cggcctgata
     5521 tatgccgtta ttacaaaaag aggatcaacc acactgcctt ttggaccgtg tttaagtctg
     5581 ggcggtatag caacacttta tctacaggca ttgttttaat gataaccacg tcattatcga
     5641 agtgacattt taactcttat taataacctt agagattatt taccatgtcg ataaaacaga
     5701 tgccagggag ggtattaata tcgctattgt tgagcgttac aggattatta agtggctgcg
     5761 ccagccataa tgaaaacgcc agtttactgg cgaaaaaaca ggcgcaaaat atcagccaaa
     5821 acctgccgat taaatctgcg ggatatacct tagtgctggc gcaaagtagc ggcacgacgg
     5881 taaaaatgac cattatcagc gaagcgggtg ctcagaccac gcagacgcct gatgcctttt
     5941 taaccagcta tcaacgacaa atgtgcgctg acccgacggt gaaattaatg atcaccgagg
     6001 gaattaatta cagcataacg attaatgata cacgtacagg taaccagtat cagcggaaac
     6061 tggatcgcac tacctgtgga atagtcaaag cataacgtcg ggtagatata aattggcgcg
     6121 ggttgttttt cgtgacgcac gaatttatct cattcagtgg ctgacaaaaa ttcgtcacac
     6181 tcttagccag agacaatctc ttaatacaga ccaagagcat ctgcgcaaaa ttgctcgcgg
     6241 gatgttctgg ctgatgctgc ttattatttc tgcaaaaatg gcgtattcac tctggcgcta
     6301 tttctccttt tctgcggaat atacggcggt ttctcaatcg gtgaataaac cgacccgtgc
     6361 ggatgcaaaa gcgttcgata aaaatgacgt gcaattaatc agccagcaaa actggtttgg
     6421 caaatatcat cccgtcgccg caccggtaaa acaacccgaa cctgcacctg tggccgaaac
     6481 gcgtcttaat gtggtgctgc gcgggatcgc ctttggtgcc agacccggcg cggttattga
     6541 agaaggtggc aaacagcagg tctatttgca gggtgaaacg ctgggctcgc acaacgcagt
     6601 gattgaggaa atcaaccgcg accatgtgat gctgcgctat cagggaaaaa tagagcgtct
     6661 gagtctggct gaagaggagc gttccacggt tgctgtgacc cgccaaaaag ccatcagtga
     6721 cgaagcaaag caagctgttg ctgaacctgc tgtcagtgcg ccagttgaga tcccgactgc
     6781 cgtgcgtcag gcactggcga aagatccgca gaaaattttt aactatatcc agcttacgcc
     6841 tgtgcgtaag gagggaattg tcggttatgc agtgaagccg ggggcagatc gttctctgtt
     6901 cgatgccagc ggtttcaagg aaggcgatat cgccattgcg ctaaatcagc aggatttcac
     6961 tgatccacga gcaatgattg ctctgatgcg gcagttacct tcaatggatt ccattcaact
     7021 tacggtttta cgcaagggtg cgcgccacga catttccatc gcgctgcgct aaccgcattt
     7081 aatccaggag aatcattcat cgtgttttgg cgtgatatta cgttgtcggt ctggcgtaag
     7141 aagacaactt gcctcaaaac aaaaaagcgt ttactgccgc tggtgttggc agcggcatta
     7201 tgcagttcac cggtctgggc ggaagaagcc actttcaccg ctaattttaa agataccgac
     7261 ctgaaatcgt tcatcgaaac cgtcggcgct aaccttaata aaaccatcat tatggggccg
     7321 ggcgtacagg ggaaagtgag tattcgcacc atgaccccgc tcaatgaacg ccagtattac
     7381 cagctattcc ttaacctgct ggaagcacag gggtatgccg tcgtaccgat ggaaaacgac
     7441 gtgctgaagg tggtgaaatc cagcgctgcg aaagtcgagc cactgccgct ggtcggtgaa
     7501 ggcagcgaca actacgcggg cgatgaaatg gtcaccaaag tagtgccggt acgtaatgtg
     7561 tcggtacgcg agctggcacc gattctgcgc cagatgatcg acagcgcagg ctcaggcaac
     7621 gttgttaatt acgatccctc caacgtgatt atgctcaccg gacgcgcctc cgttgtggag
     7681 cgcctgacgg aagtgatcca gcgcgtggat cacgcgggta atcgcaccga agaggtgatc
     7741 ccgctggata acgcctcggc ttcggaaatc gcccgcgtgc tggaaagcct gacgaaaaac
     7801 agcggcgaga accagcctgc gacgctgaaa tctcaaattg tcgccgacga acgcaccaac
     7861 agtgtgattg tcagtggtga cccggccacg cgggacaaaa tgcgccgcct gatccgtcgc
     7921 ctggactcag aaatggagcg cagcggcaac agccaggtgt tctatctcaa atacagcaaa
     7981 gccgaagatc tggtcgatgt actgaagcag gtcagcggca cgctcacggc ggctaaagaa
     8041 gaggcggaag gcacggtcgg tagcgggcgt gaggttgtct ccatcgccgc cagcaaacac
     8101 agtaatgccc tgattgttac tgcgccgcag gacatcatgc agtctctgca aagcgtgatt
     8161 gaacaactgg atattcgccg tgctcaggtg cacgtcgagg cgttgatcgt ggaagttgcc
     8221 gaaggtagca atatcaattt cggcgtgcag tgggcgtcga aagatgccgg attaatgcag
     8281 tttgctaatg gtacgcagat ccctatcggt acgctgggcg cagccatttc tcaggcgaaa
     8341 ccgcagaaag gctcgacggt gatcagtgaa aacggcgcta ccaccattaa tccggatacc
     8401 aacggcgatc tctccacgct cgcccagctt ctttccggct ttagcggtac ggcggttggt
     8461 gtggtgaaag gcgactggat ggcgctggtg caggcggtta aaaacgactc cagctctaac
     8521 gtgctctcca cgccgagcat caccacgctg gacaaccagg aagccttctt catggtgggt
     8581 caggacgttc cggtattaac cggctctacc gttggctcca ataacagcaa tcctttcaat
     8641 acagtagaaa ggaaaaaagt cggcatcatg ctgaaagtca cgccgcagat taacgaaggt
     8701 aacgcggtac agatggtgat tgagcaggaa gtctcgaagg tggaaggaca gaccagcctc
     8761 gacgtggtgt ttggtgagcg caaactgaaa accaccgtgc tggctaacga tggtgagcta
     8821 attgtgcttg gcggtctgat ggacgaccag gcgggagaaa gcgtggcgaa agtgccgctg
     8881 cttggcgata tcccattgat tggtaacctg tttaaatcga cggcggataa aaaagaaaaa
     8941 cgtaacctga tggtgtttat ccgtccgacc attctgcgtg acggtatggc ggcagacggc
     9001 gtgtcgcagc gcaaatataa ctatatgcgc gccgaacaga tctatcgtga tgagcaaggc
     9061 ttaagcctga tgccgcacac cgcgcagccg gtactgccag cgcaaaacca ggccttaccg
     9121 ccggaagtcc gtgcgttcct taatgccggg agaacgcgtt aatggtgcct gtagcacagg
     9181 aaaccaccgc caacaccgtg cgtctgccct acagtttcag ccgtcggttt agcctggtgg
     9241 catggtgcga agcgtcgctg gagatcctcc acgttcatcc gctatcgctc tctgttttgc
     9301 aggagctgca gcgggggctg aacgcgccct ttacgctgcg gcaaatcgac gaggccgaat
     9361 ttgaacagcg gctgaatgcg gtctggcagc gggactcttc cgaggcccgc cagctaatgg
     9421 aagatctcgg ttctgctgag gactttttta ctctcgctga agaactgccg gaaacggaag
     9481 atctgctgga aagtgacgac gatgcgccga tcatcaaact gatcaacgcc atgctggcag
     9541 aggcgattaa agaaggcgct tcggatattc acatcgagac gtttgaaaag agtctggtga
     9601 tccgttttcg cgttgacggc acattacatg aaatgctgcg ccctgggcgc aaactggcct
     9661 ctctgctggt atcgcgtatt aaggtgatgg cacggctgga tatcgccgaa aagcgcgtac
     9721 cgcaggatgg acgtattgcg ctgctgctgg gcggtcgggc gattgacgtg cgtgtctcca
     9781 ccatgccttc tgcctggggc gagcgcgtgg tgctgcgact gctggacaaa aaccaggccc
     9841 gcctgacgct ggagcgtctg gggcttagcc agcaactgac cgcgcagttg cgccagctgt
     9901 tacacaaacc gcacggcatc tttctggtga cggggccgac gggttccggc aaaagcacca
     9961 cgctgtacgc cggattgcag gagctgaaca accattcgcg caacattctc acggttgaag
    10021 accccatcga atacatgatt gaagggatcg gtcagacgca ggttaacacc cgcgtcggca
    10081 tgaccttcgc ccgtggcctg cgcgcgattt tgcgtcagga cccggatgtg gtgatggtcg
    10141 gtgaaatccg cgataccgaa accgcagaaa ttgccgtcca ggcatcgctt acagggcacc
    10201 tggtactttc cacgctgcat accaacacgg cggtgggggc gatcacgcgt ttgcaggata
    10261 tgggcgtgga gcctttcctg ctctcttcca gtctgactgg cgtgatggcg cagcgactgg
    10321 ttcgtacgct gtgccccgac tgccgccagg ccgcgcctgc cactgacgaa gaaaaacgcc
    10381 tgctgggaat taccgacgcc cgtaccgtca ctctgtacca tccgcagggc tgccccgcct
    10441 gtaatcacaa aggttttcgc ggacggactg ccatccatga gctgatcgtg gtggatgcca
    10501 cattgcgtga tttgatccac cgtcaggccg gggagctgga gctggaacgt tatgtccgac
    10561 aacactctgc gggtatccgc agtaacggca ttgagaaagt gctcgccgga gaaacctctc
    10621 tcgatgaagt tctgcgggta accatggagg cgtaatggca ctgttttact atcaggcgct
    10681 ggagcgtaat ggtcgcaaaa acaaaggcat gattgaggcg gattccgcgc gtcatgcccg
    10741 tcagttgttg cgcgctaaag agcttatccc tgtgaacatt gaagcccgga tgaatgcttc
    10801 gtcaggggga atattgcagc gtcggcggca cgcacatcgt cgcgtggcgg cggcagatct
    10861 ggcgctgttc acgcgccaac tggcaacgtt agtacaggca gcaatgccgc tggaaacctg
    10921 cttacaggcg gtcagtgagc aaagtgaaaa actgcatgta aaaagcctcg gaatggcgct
    10981 gcgcagccgg attcaggaag gttacaccct gtcggacagc ctgcgcgaac atccccgcgt
    11041 ctttgattcc ctgttttgtt cgatggtggc tgccggagaa aaatccgggc atctcgacgt
    11101 ggtgcttaat cgcctggcgg attacaccga acaacgccag cgcctgaaat cacgcctgct
    11161 gcaggccatg ctctatccgc tggtactgct ggtggtggca acgggcgtgg tcactatttt
    11221 gctgacggca gtggtgccga aaatcatcga acagtttgat catctcggac acgcgctgcc
    11281 cgcctctacc cgcacgctta ttgccatgag cgacgcgtta caggccagcg gcgtgtactg
    11341 gctggcaggc ctgctggggc ttctggtgct ggggcaacgg ctactcaaaa atcctgcgat
    11401 gcgcctgcgc tgggataaaa ccttgctgcg cctgcctgtg acggggcgtg ttgcgcgcgg
    11461 gctgaatacg gcgcggtttt cccgcacatt aagcattctc accgccagca gtgttccgct
    11521 actggaaggc attcagaccg ctgccgctgt gtcggcaaat cgctatgtcg agcaacaact
    11581 gctgctggcg gcagatcgcg tccgcgaagg aagcagtctg cgcgccgcgc tggcggagtt
    11641 gcgcctgttc cccccgatga tgctgtacat gatcgcctcc ggcgaacaga gcggcgaact
    11701 ggaaaccatg cttgaacagg ccgcagttaa ccaggagcgg gaatttgata cccaggtggg
    11761 gctggcgttg gggctgtttg agccggcgct ggtggtgatg atggcgggcg tggtgctgtt
    11821 tatcgtcatc gccatcctcg agccgatgct gcaactgaac aatatggttg gaatgtaatt
    11881 tacggagtta tcacatgaat tcgttatccc gcacacaaaa accacgggca ggttttaccc
    11941 tgctggaagt gatggtggtg attgttattc ttggcgtcct ggcaagtctg gtggtgccta
    12001 acctgttggg caataaagag aaagcggatc ggcaaaaagc catcagcgat atcgtggcgc
    12061 tggaaaacgc actggatatg taccgactgg ataacgggcg ttatccgacc acagaacagg
    12121 ggcttgaggc gctgatccag caaccggcca atatggcgga ttcccgcaac taccgtaccg
    12181 gtgggtacat taaacgactg ccaaaagatc cgtggggcaa tgattatcag tatctcagtc
    12241 ctggtgaaaa aggactgttt gatgtttaca ccctgggggc ggatggtcag gaaaatgggg
    12301 aaggcgctgg cgcagatatc ggtaactgga atttgcagga gtttcagtaa gcagtgcctg
    12361 aacgcggatt cacacttctg gaaatcatgc tggtgatttt ccttatcggc cttgccagtg
    12421 cgggcgtggt acagacgttt gcgaccgatt ctgaatcgcc cgcgaaaaaa gcggcgcagg
    12481 attttctgac tcgctttgcg cagtttaaag acagggcagt gatcgaaggg cagacactcg
    12541 gtgtactaat cgacccgccg ggataccagt ttatgcagcg tcgtcagagg cagtggttgc
    12601 ccgtttctgc gaccaggtta tcggcacagg ttacggtgcc gaaacaggtg cagatgctgt
    12661 tacaacccgg aagtgatatc tggcagaagg aatatgcgct ggagctgcaa cgtcgtcgcc
    12721 tgacgctgca cgatattgaa ctggagttgc aaaaagaggc gaaaaagaag acgccgcaga
    12781 tccgtttttc gccttttgaa cccgccacgc cgtttacgct gcgcttctac tcagcggcgc
    12841 aaaacgcatg ttgggcggta aaactggcac acgatggcgc gttatccctc agtcaatgtg
    12901 atgagaggat gccatgaagc gcggatttac cttgctggaa gtgatgctcg cgctggcgat
    12961 ttttgcgctg gctgccacgg cggtgttaca gattgccagc ggtgcgctga gtaatcagca
    13021 cgttcttgag gagaaaacgg tagcaggctg ggtggctgaa aaccagaccg cgctgctcta
    13081 cctgatgacc cgtgaacaac gggcggtcag gcatcagggc gagagcgata tggctggaag
    13141 ccgctggtac tggcgaacca caccgcagaa tacaggcaac gcgctgctcc aggcggtgga
    13201 tattgaagtc agccttcacg aagacttttc gtcggtaatt cagtcacgac gcgcctggtt
    13261 tagcgccgtg ggaggccaac agtgagaagg acttgcgctg gtttcacgtt actggaaatg
    13321 ctggtggcaa ttgccatttt tgcctcgctg gcactgatgg cgcagcaggt gacaaacggc
    13381 gtcacacgcg tgaatagtgc cgtcgccgga cacgatcaaa aactaaacct catgcagcaa
    13441 acgatgagtt ttctgaccca cgatctgacc caaatgatgc cgcgtccggt aagaggcgat
    13501 cagggccagc gagaaccagc gttactggcg ggcgctggcg tgctggcgtc tgagagtgaa
    13561 ggaatgcgct ttgtacgcgg tggcgtggtt aatccgttga tgcgtctgcc acgcagcaat
    13621 ctgctcaccg tcggttaccg cattcatgac ggttatctcg aacggttagc ctggccgctg
    13681 accgatgccg caggcagcgt gaagccgaca atgcaaaagt tgattccggc cgattcgctc
    13741 cgtttgcagt tctatgatgg tacgcgctgg caggagaact ggtcgtcagt acaggcgatc
    13801 cctgtggcgg tgcgcatgac cctgcattcg ccgcaatggg gcgagattga acgcatctgg
    13861 ttgttacgcg ggccgcaatt atcatgatca cctcaccacc aaaacgcgga atggcactgg
    13921 ttgtggtgct ggtattgctg gcggttatga tgctggtaac catcacgctt tccgggcgga
    13981 tgcagcaaca acttgggcga acccgcagcc agcaggagta ccagcaggcg ctgtggtaca
    14041 gcgccagtgc agaaagcctg gcgctgagtg cactcagtct gagcctgaaa aatgaaaagc
    14101 gcgtgcatct ggcacagccg tgggcttccg gccctcgttt tttcccactg ccgcaggggc
    14161 aaatcgccgt cactctgcgt gacgcacagg cgtgctttaa cctgaatgtc ctcgctcagc
    14221 cgacgacggc gtcgcgtccg ctcgcgttac aacaactgat tgccctgatc tcgcgcctca
    14281 atgtgcctgc ttatcgggcc gaactgatag ccgaaagcct gtgggagttt attgatgaag
    14341 accgcagcgt gcagacgcgt ctgggccgtg aagacagcga gtatctcgcc cgttcggtgc
    14401 cgttttacgc cgctaatcaa ccgctggctg atatcagcga gatgcgcgtg gtgcagggaa
    14461 tggacgccgg actttatcaa aaactgaaac cgctggtctg tgcgctgccg atgacccgcc
    14521 agcaaatcaa catcaacacc ctggacgtca cgcaaagcgt gattcttgag gcgctgtttg
    14581 acccgtggtt aagccctgta caggcgcggg cgttattaca acaacgtccg gcgaagggct
    14641 gggaagatgt cgatcagttt cttgcacagc cgctacttgc tgacgtcgat gagcgtacta
    14701 aaaaacagct aaaaaccgtc ctgagcgtgg acagcaatta cttctggctg cgttcagata
    14761 tcaccgtgaa tgagattgaa ctgacgatga actcgttaat tgtccgcatg ggcccacaac
    14821 acttttcggt tctctggcat cagacaggag aaagtgagtg agttccatgc ttgagatttt
    14881 tttcccgctt tgcgcctccg acaccatccg ctggcagcgc cgtacacccg acgtggagca
    14941 cggtatctgg cctgacgttg ctgacgaacg tctccagcaa tggctgcaaa ctgatgcgat
    15001 tcgactctac attcctggcg aatggatcag cgtctggcag gttgaactgc ctgatgttgc
    15061 ccgcaagcag ataccgacta ttctgcctgc cttattggaa gaagagctga atcaggatat
    15121 cgacgaactg catttcgccc tgttgaaaat cgaccagcaa caggcaaccg tggcagtgat
    15181 tcaccagcag catatgcaca acattgcgca gtggttgcag gaaaacggta tcacccgcgc
    15241 taccgtcgcg ccggactgga tgtctattcc gtgtggctat atggctggcg atgcgcaacg
    15301 ggttatctgc cgcatcaatg agtgccgggg atggagcgcc gggtgggcgc tggctccggt
    15361 catgttccgc gcacagctca atgagcagga cttaccgatt tcactaaccg tggtcggcat
    15421 tgcaccggaa gaactatctg catgggctgg tgcggacgcc gaacgcctga ccgttacggc
    15481 tctgccagcc attaccactt atggtgaacc ggaagggaac ctgctaacag ggccgtggca
    15541 gcctcgcgtc agctaccgaa aacagtgggc gcgctggcgg gtgatgattc tgccgatatt
    15601 gctgattctg gttgcgctgg cagtggaacg gggcgtgacg ttatggagcg tcagcgaaca
    15661 ggtggcgcaa agccgcaccc aggcggaaaa acagttctta acactgttcc cggagcagaa
    15721 gcggattgtg aacttacgct ctcaggtgac gatggcgctg aaaaaatatc gcccgcaggc
    15781 cgacgatacc cggctgctcg ccgagttgtc agcgatagcc agcaccctga aatcagcgtc
    15841 actttccgac atcgaaatgc gtggtttcac ctttgatcaa aaacgccaga cgcttcacct
    15901 ccagttgcgg gcagcaaact ttgccagctt cgacaaactg cgtagtgcac tggcaaccga
    15961 ttatgttgtg caacaggacg cgttacagaa agagggtgat gcggtttccg gcggcgtaac
    16021 gttgcggagg aaataacatg ttacgcgata aatttattca ctattttcag caatggcgtg
    16081 aacgccagtt aagccgtggc gaacactggc tggcacaaca cctggcgggg cgttctccgc
    16141 gtgaaaaggg catgttactg gcagcggtgg tgttcctgtt tagcgtcgga tattacgttc
    16201 tcttctggca gccgttgagc gaacggatag aacaacagga gacgatgttg cagcagctgg
    16261 tggcaatgaa cacccgactg aagcgcgccg cgccggatat tatcgcagcg cgaaaatccg
    16321 ccacgacagc acctgcgcag gtatcgcggg tcatcagtga cagtgcttcc gcgcactcgg
    16381 tggtcatcaa gcggatagcc gagcgtgggg agaatattca ggtctggata gaacctgtgg
    16441 tgtttaatga cctgctgaaa tggttaaacg cactggatga aaaatatgcg ctgcgggtga
    16501 cacaaattga tgtcagtgct gctgagaagc ctgggatggt gaatgtgcag cggctggagt
    16561 ttggacgggg gtaaagtatc aggagattaa tatcaataaa attactctcg ttaacgtggc
    16621 gagagtaatt cccccagact ttataagcta atttttttcg tgaaggttat tgtcagatcc
    16681 aaaagcaaaa acccgccttg tgggcgggtt cttaagaatt agtggtgccc ggactcggaa
    16741 tcgaaccaag gacacgggga ttttcaatcc cctgctctac cgactgagct atccgggcaa
    16801 cggggcgcat taaacctgat tcgcctcgtc tcgtcaatca aatttcttca atttactgca
    16861 gactgcacaa tctatcatca ctttgccgat attgcacgca ttgtcaggca agccaggcgg
    16921 tccaggcggc ggaaagcggc attgccagaa ggagtgctgg aagcatgttg accaccggga
    16981 acattttaat gccacagatg cgtaatccgg ttgccagcag caataaaccg cctacggcgc
    17041 tgaagtctgc catcatcgac ggtgtggtca gcggtaatat cagcgcggca gcccacgcca
    17101 gcgttaactg gatgatcagt aatgggatac taatcaccga taccgcaata cccagcgagc
    17161 aggcgaagat catcgccgta aagaaatcaa gaaatgactt ggcgattaaa atactcggat
    17221 cgccggtcat cccttcgttc atcgccccga agatcccggt gccgctggcg caaaacagga
    17281 caataatcgc gacaaaattc tgaatgaaag attcatgcgc tggcttctta cgtgagtgac
    17341 gaaacagatt ttgtgctttg gcgaccgctg tattgacgcc tttttccagc agacaaattt
    17401 cgccgattaa tgctccgagt aaggttgcta aaaccatcgc tggaaggttg gcgcatttca
    17461 ccaccagtaa aataccaatc cccagcgatg ccagaccaaa aattgatgtc atggagacgc
    17521 ggatacgttc cggtaagcgt tggctaagca gtgcaccgag catgccaccc agtaatattg
    17581 cacttgcgtt gataaaaggg ccgatgacca caagagttcc tgttaactgt attgtttgat
    17641 tttcttatta tgaacttttt gccttgttga tggcttgtct ggtcgcaaag tcctggcttg
    17701 cacgtcttag cgaaaggtgc catgattgcg cgaattttct cctctctgta cggagtttgc
    17761 ccgatgcacg ccatctcctt acattctctc gcttatcgcc gtttcgcgcg aaacgtttcc
    17821 ctttctatgt tactgctcat gcggtgaagt taacgcacgc tcactgcagg acaacagtaa
    17881 aatcagagcg tttctgcttt tactgatgtc tggcggtcgg agctggtgac cagtttgacc
    17941 catatctcat ggggcagggt tttacacctt gtccggtatt cttacttccc cgaaacgggt
    18001 ttgcgcttat gaaatcaatg aatattgccg ccagtagtga actggtatcc cgactttcta
    18061 ctcatcgccg cgtggtggcg ttgggagata ctgattttac ggacgtcgcg gcagtcgtca
    18121 ttaccgctgc ggatagtcgc agtggcattc ttgcgttgct taagcgcacc ggttttcatc
    18181 taccggtgtt tttgtattcc gaacatgctg ttgaattacc tgcgggcgtt acggcggtaa
    18241 tcaacggcaa cgagcagcag tggctggagc tggaatccgc agcctgtcag tatgaagaga
    18301 atttgctgcc accgttttat gacacgctga cgcagtacgt tgagatgggc aacagcacct
    18361 ttgcttgccc tggacatcaa catggtgcgt tttttaaaaa gcatcctgcc ggacgccatt
    18421 tttacgattt ctttggtgag aacgtctttc gcgccgatat gtgtaatgct gacgtaaaat
    18481 tgggcgatct gcttattcat gaaggatcgg cgaaagatgc gcagaaattc gcagccaaag
    18541 tctttcatgc cgataaaacc tattttgtgc tgaacggcac atcggcagcg aataaagtgg
    18601 tgacgaatgc gctgttaacg cgtggcgatc tggtgctctt cgaccgtaac aaccataagt
    18661 cgaatcatca cggcgcgctg attcaggcgg gggcgacgcc ggtctatctg gaagcttcac
    18721 gtaacccgtt tggtttcatt ggcggtattg atgcgcactg ttttaatgaa gagtatctgc
    18781 gccagcaaat tcgcgacgtt gcgccagaaa aagctgagtt gccgcgcccg tttcgcctgg
    18841 cgattattca gctgggaacc tatgacggca ctgtctataa cgcccgtcag gtgatcgata
    18901 ccgttgggca tctgtgtgat tacattctgt ttgattccgc gtgggtcggt tatgaacaat
    18961 ttatcccgat gatggcggat agctcgccgc tgctgttaga acttaacgaa aacgatccgg
    19021 ggatttttgt gacccagtcg gtgcacaaac agcaggctgg attctcacag acgtcgcaga
    19081 tccataaaaa agataaccat atccgcggac aggcgcgttt ttgcccgcat aagcggttga
    19141 ataacgcctt tatgctccat gcttctacca gccctttcta tccgctgttt gctgcactgg
    19201 atgttaacgc caaaattcat gaaggggaga gtgggcgtcg gctgtgggct gagtgcgttg
    19261 agttgggtat tgagtcgcgc aaggctattc ttgcgcgctg taagctgttc cgcccgttta
    19321 tcccgcccgt tgttgatggc aaattgtggc aggattatcc gacatcagtg ttagccagcg
    19381 accgccgttt tttcagtttt gagccggggg cgaagtggca cggctttgaa ggatatgccg
    19441 cggatcagta ttttgttgat ccgtgcaagc tgttactcac tacaccaggt atcgatgccg
    19501 aaaccggcga atatagcgac tttggcgttc cggcgacgat tctggcgcac tatctgcgtg
    19561 agaacggcat tgtgccggag aagtgcgatc tcaactccat tctgttctta ttaactccgg
    19621 cggaaagcca cgagaagctg gcacaactgg tggcgatgct ggcgcaattt gaacagcata
    19681 ttgaggatga ctcgccgctg gttgaggtgt tgccgagcgt ttataacaaa tatccggtgc
    19741 gctatcgcga ctacaccctg cgccagttgt gtcaggagat gcacgatctg tatgtcagtt
    19801 tcgacgtcaa agacctacaa aaagcgatgt tccgccagca gagtttcccg tcagtggtga
    19861 tgaaccccca ggatgcgcat agcgcttata ttcgcggtga cgtggagttg gtgcggattc
    19921 gtgatgccga agggcgaatt gcggcagaag gggcgttgcc ttatccacct ggcgtgcttt
    19981 gcgtggtacc cggggaagtc tggggtgggg cggttcaacg ttatttcctt gcactggaag
    20041 aaggggtgaa tttgttgccg ggattttcgc cggagctgca aggtgtttat agcgaaaccg
    20101 atgcggatgg catgaaacgg ttgtacggtt atgtgttgaa gtaagaataa aaaaaacggg
    20161 tcgccttctg gcgacccgtt tttctttgcg taattagtgg ctaaccgtct gtgtacctgt
    20221 cgggacacga acgtgtttat atttgaacat cgccatgaac gcgaaggcca gaaccacgga
    20281 gtaaccagcg aaaatcaacc atacggtctg ccagtcggta atgccgtttt gggtgtacat
    20341 ctcaaccact ttaccgctca cgatgccgcc gaggatacag ccgaagccgt tggtcatcat
    20401 caggaacatc ccttgcgcac tggcgcgaat cgccgggcta acttcttttt cgacaaacac
    20461 cgaaccagag atgttgaaga agtcgaacgc acagccgtaa acaatcatcg acagaaccag
    20521 cagtacggtg ccgaacggag tcgggtcgcc gtaagcaaac agcgcaaaac gcaggatcca
    20581 cgccacgata ctgatcatca ttacgttctt aatgccgtag cggcttaaga agaacgggat
    20641 ggtcagaatg aacagggttt cagagatctg cgaaatcgac atgatgattg acgcatgctg
    20701 cacaataaag ctgctggcaa acatcggatc tttgtcgaag ctgtgcagga aggtattacc
    20761 gaacatgttg gtaatctgca gttccgcgcc cagcagcatt gagaagatga agaagattgc
    20821 catacgcttg tttttaaaca gcgcgaatgc atcgaggccc agcagggttg tccagctctg
    20881 attcgcttgc tgtttagcaa ccggaatatg cggcagagtc agggtaaaca gaaccagaat
    20941 ggcggaaagt gctgcgccaa tatacagctg catgtggctt aattcgaagc cagacaggct
    21001 caccacccac attgccatga taaagccgat ggtgccccag atacggattg gcgggaagtc
    21061 agtaacgata tccatcccgg cattttgcag gcgatagtaa gagatggtgt tgattaaccc
    21121 aagcgttggc atataagcaa acgagttaat caatatcaca aggaacatcg cttccggcgt
    21181 cgtgacctgt gccgccatga acagcgtgat agcgccaatg gtgtggcaaa tggcatatac
    21241 ccatttcgca cttaaccatt tgtcggccac aatccccagc agcgcaggca taaagaccgc
    21301 ggcgataccc agtgagctat aaactgcgcc aatagaagca ccgtcaaact tcagggtaac
    21361 aaacatatag gaaccgaggg tcgtcagcca acttccccac agacagaact gcagaaaaga
    21421 gaggattttc agctgcagct taagattcat gttaatttcc tcacatcgtg atgcggatga
    21481 gtgtttgaaa aagcacattt gaccgtgcga gatgatggga ttctatcaac ttcgcggact
    21541 ggttttttgt tacctgtggc aaataattgc aaacattccc aatttgcaaa acgaaattgt
    21601 gatgtggata acatttttac ccctgagcat cgtcaggggc ggttaatgga attatcggcg
    21661 gcggtaggat ttttgcgcgg tattcacttt ataaagatag cggcgagatt ccgcagaggg
    21721 atgacgggtc gtcagcgtct gataaacatc gcctggcgtc atggtgttaa taatattagc
    21781 cgcctgaatc ttgtcattcg aaaagactct cagcacgctg cctgcgccac cgttataggc
    21841 ggtgatgacg gcataacgcc gtgacgttgg gttatcaatt ccgccgagat aaacattgtt
    21901 cagcatcgcc agatacgcgg tgccggtatc aatattgctg gcaggatcaa acaagaaact
    21961 gcggctcggc gtgccggatt ttccctgcga gcggaacaca tctttcccgg cagtatgttg
    22021 taccacctgc attaatccca gcgcatcgga acggctgacc gcatacgggt taaaggaaga
    22081 ttcggtctgc ataattgcca gaatcagcga ctcatcaacg ccatatttcc gtgacgcctg
    22141 gcggaccatg ccgagatatt tgtgcgcacg tttatcaagg tggttcggta ccatgttaat
    22201 ggtgacgctg tagatgatac gcagcccgtt actgcggctt tgcagacggt ttttcagcag
    22261 ataatccgcg aagttgcttg cgcgaccttc ccagcgaatc ggctgcccgg tgttgtccac
    22321 cacctgaccg taaaggaaag gttctttcga aatcgtaata tcatcaacgt cggaatagag
    22381 atcgaccgaa ctcggatcgt cacccatcaa taacgttttg ataattgccc ggcgcaaatg
    22441 cgcggcaggt tctgtcccgg cgatggtttc gatagtaatc gtaccgtcat cgaagttgat
    22501 gtggctgcgg gtctgatatt gatcggtgta tttcacgtag tccttaggac cggcgatcac
    22561 cacctctttg aatccccaga tgttctcaat attgtgggca aattgcccca tcagaatatc
    22621 aaaaccgttg gtgtctttga cccaggcttc gttataggta tcgccttttt tggtcgtcga
    22681 acaggagatg agcaacggcg caatcaaagc cagcgcgaga tattttttca tcattccggg
    22741 tgcgtgttgt gtttgttttt atgcaagttg ccggaggcgt gctccggcac tgtttttatt
    22801 ttttatcttc cggcgtatag ccctcgatat gcacctcttt accctcgaac aggaagttga
    22861 ccatctcctg ctccagcagc ttgcggtgct cggcattcat catgttgagt ttcttttcat
    22921 taatcagcat ggtttgtttg tgctgccact gcgcccaggc ttctttggag atctcgttat
    22981 agatgcgttt tcccagttcg ccggggtaca gctgaaaatc ctgaccttct gcttcacgtt
    23041 gcaggaaagt acaaaaaatc gttctgctca taaatcatcc tctttatcga ctcacgcgct
    23101 aaaccggcgc gccagtgcgt aactgctgta acaaacgctc cacgggagcc gccaggccaa
    23161 ctgacggcgg ttgcgctaag ttataccaga gcgcattgcc ttcatccatg cagccggtga
    23221 atgacgacac gggaagccac ataggcacaa tatctaagtg gaaatggctg aaggtatgcc
    23281 gaaacgcggt cagctgcgtc aggttatcgg cagcaatctg ccgttgcgcc agccactgcc
    23341 gcaaactttc ttcgtcggca aactgcggga aacagtataa accgccccac aatccgctcg
    23401 gcggacgctg cgccagcaat acttcatctt cgtgctgtaa caggagtaag tagccggtgc
    23461 gctccggcag cgtctgtttc ggttttttgc ccggataaag cgaccagcta ttgttggtgg
    23521 cggcaataca tccgttttgt agcggacaga gcgaacattt cggcttcgaa cgcgtgcaaa
    23581 tcatcgcgcc caaatccatc atcgcctgat taaaccgttc cacgccgacc gcgggcgtca
    23641 cctgctcgct taaactccat aatttattct cgacctcttt tttcccaggc cagccgctta
    23701 cagcatagca gcgcgccagc acccgtttga cgttaccgtc gagaatcgga aagtgcttac
    23761 ccagagaaag cgagagaatc gcgcctgcgg tggaacgccc gacgcccggc agtgctgcaa
    23821 cttcctcaaa ggtttccggg aatttaccgc cgtgtaaggt cgccacttgt tgtgccgctt
    23881 tatgcagatt gcgcgcgcgg gcgtaatagc caagcccggt ccacaagtgg agaacttcgt
    23941 cgagcggcgc attggcgaga tcggtcaccg tcgggaagcg cgccataaag cgttcaaaat
    24001 aggggataac ggtcgcaacc tgagtttgtt gcaacatcac ttctgagagc catactttgt
    24061 agggcgtctt gtcaatttgc cagggcagag tttttcgccc gtatttatcg taccagtcca
    24121 gaacctgggc tgaaaattgc gacgcttgca tggtcaccga attcactgtt gttgggggta
    24181 agattgcagc acagcgacgg cagggtgtaa accggaactt tccgcagcca cggccttatc
    24241 atgcttgcat ccggcaacta actttggata atgcccgttt tcagaacact ttcacaagcg
    24301 actaaatctt tatgaaaaac gacgtcattt caccggaatt tgatgaaaac ggccgcccac
    24361 tgcgccgtat ccgtagtttt gtgcgccgcc aggggcgact gaccaaaggc caggaacatg
    24421 cgctggaaaa ctactggccg gtgatgggcg ttgagttcag cgaagatatg ctggatttcc
    24481 ccgcgctttt tggccgtgaa gcgccggtga cgcttgagat tggttttggc atgggggcgt
    24541 cgctggtggc aatggctaaa gatcgccccg agcaggactt cctcggcatc gaagtgcatt
    24601 caccgggcgt tggtgcgtgc ctggcttctg cgcatgaaga gggcttaagc aacctgcgcg
    24661 tgatgtgtca cgatgcggtt gaagtgctgc ataaaatgat tcctgacaat tcattgcgca
    24721 tggtgcagct ctttttccct gacccgtggc acaaagcgcg ccataataaa cgccgtatcg
    24781 ttcaggtgcc gtttgccgaa ctggtaaaaa gcaaactgca gctggggggc gtattccata
    24841 tggcgaccga ctgggaacct tatgcggaac atatgcttga agtgatgtct tctattgacg
    24901 gttataaaaa cctgtcagag agcaatgatt acgtaccgcg tccggcatca cgtccggtga
    24961 cgaaatttga acaacgtggt catcgtcttg gtcacggagt atgggactta atgttcgaga
    25021 gggtgaaata atggcaaaga accgtagccg tcgtctgcgt aaaaaaatgc acatcgacga
    25081 attccaggaa ttaggatttt cggtggcatg gcgattcccg gaaggtacat cggaagaaca
    25141 gattgataaa accgttgatg attttattaa cgaggttatc gaaccgaaca aactggcctt
    25201 tgacggcagc ggttatctgg cctgggaagg tctgatctgc atgcaggaaa tcggcaaatg
    25261 caccgaagaa catcaggcga ttgtgcgtaa gtggctggaa gagcgcaaac tggatgaggt
    25321 acgcaccagc gaacttttcg acgtttggtg ggactaagaa agcatacggg cgatgacaaa
    25381 tgcaaaactg cctgatgcgc tacgcttatc atgcctacaa agatgcacga tcgagtaggc
    25441 gggataaggc gtttacgccg catccggcat ggaaaacgcg tactttgtta tcaatctggg
    25501 gccagcaaat gctggcctga tttgttcttg agggaagact atgatgcgca aaatgctgct
    25561 ggcggcagca ctttcagtga cggcaattac cgctcacgcc gactaccagt gcagcgtcac
    25621 gccgcgtgac gatgtgattg tcagcccgca aaccgtgcag gtgaagggcg aaaacggcaa
    25681 tctggtgatc acgccagacg gcaacgtgat gtataacggt aagcaatatt ctctgaatgc
    25741 cgcccagcgc gagcaggcga aggattatca ggctgaacta cgcagcacac tgccgtggat
    25801 tgatgaaggc gcgaaaagcc gcgtcgaaaa agcccgtatt gctctggata aaattatcgt
    25861 tcaggagatg ggcgaaagca gcaaaatgcg cagccgtctg accaaacttg atgcgcagct
    25921 gaaagagcag atgaaccgca ttatcgaaac gcgcagcgat ggcctgacgt ttcactataa
    25981 agccattgat caggttcgcg ccgaaggcca gcaattagtg aatcaggcaa tgggtggaat
    26041 tttacaggac agcattaatg aaatgggcgc gaaagcggtg ctgaaaagcg gcggcaaccc
    26101 attacagaat gtgctgggaa gtctgggcgg tctgcaatcc tcaatccaaa ccgagtggaa
    26161 aaagcaggaa aaagatttcc agcagtttgg caaagatgtt tgtagccgcg ttgtgactct
    26221 ggaagatagc cgcaaagccc tggtcgggaa tttaaaataa tcctctattt taagacggca
    26281 taatactttt ttatgccgtt taattcttcg ttttgttacc tgcctctaac tttgtagatc
    26341 tccaaaatat attcacgttg taaattgttt aacgtcaaat ttcccataca aagctaaggg
    26401 ataatgcgta gcgttcacgt aactggagga atgaaatgga gtttttcaaa aagacggcac
    26461 ttgccgcact ggttatgggt tttagtggtg cagcattggc attacccaat atcaccattt
    26521 tagcaaccgg cgggaccatt gccggtggtg gtgactccgc aaccaaatct aactacacag
    26581 cgggtaaagt tggcgtagaa aatctggtta atgcggtgcc gcaactgaag gacattgcga
    26641 acgttaaagg cgagcaggta gtgaatatcg gctcccagga catgaacgat aatgtctggc
    26701 tgacactggc gaaaaaaatt aacaccgact gcgataagac cgacggcttc gtcattaccc
    26761 acggtaccga cacgatggaa gaaactgctt acttcctcga cctgacggtg aaatgcgata
    26821 agccggtggt gatggtcggc gcaatgcgtc cgtccacgtc tatgagcgca gacggtccat
    26881 tcaacctgta taacgcggta gtgaccgcag ctgataaagc ctccgccaac cgtggcgtgt
    26941 tggtagtgat gaacgacacc gtgcttgatg gccgtgacgt caccaaaacc aacaccaccg
    27001 acgtagcgac cttcaagtct gttaactacg gtcctctggg ttacattcac aacggtaaga
    27061 ttgattacca gcgtaccccg gcacgtaagc acaccagcga cacgccattc gatgtctcta
    27121 agctgaatga actgccgaaa gtcggcattg tttataacta cgctaacgca tccgatcttc
    27181 cggctaaagc actggtagat gcgggctatg atggcatcgt gagcgctggt gtgggtaacg
    27241 gcaacctgta taaaaccgta tttgacaccc ttgcgaccgc cgcgaaaaac ggcactgcgg
    27301 tcgtgcgttc ttcccgcgta ccgacgggcg ctaccactca ggatgccgaa gtggatgatg
    27361 cgaaatacgg cttcgtcgcc tctggcacgt tgaacccgca aaaagcgcgc gttctgctgc
    27421 aactggctct gacgcaaacc aaagatccgc agcagatcca gcagatcttc aatcagtact
    27481 aatcacctcg ccccggtatc gtgccggggc tttttcactt cagacttact tccattgcca
    27541 atttttatta ccctgatgat aatcaccgga ataaattatt ccgcgcgagg gttttcgggt
    27601 gaaaaagcaa tggattgttg gcacggcact gcttatgttg atgactggta atgtctgggc
    27661 agatggcgaa ccgccaactg aaaatatctt aaaagatcaa ttcaaaaagc agtatcacgg
    27721 cattctcaag cttgatgcca tcaccttaaa aaatcttgat gctaagggta atcaggccac
    27781 ctggtcagcg gaaggcgatg tctcttccag tgacgatctc tatacctggg tcggtcagtt
    27841 ggcagattac gaactgctgg aacagacctg gacgaaagat aaaccggtaa aattctcggc
    27901 aatgttaacc agtaaaggaa cgcccgcgtc tggctggtcg gtgaactttt actcttttca
    27961 ggcggcagcc agcgatcgtg gtcgggtggt cgacgatatc aaaacgaata ataaatacct
    28021 gatcgtgaat agcgaagatt tcaattatcg ttttagtcag cttgagtcag cgttgaatac
    28081 ccagaagaac tcgattcctg cgctggagaa ggaagtgaaa gagcttgata agaaaatggc
    28141 agcggcgcag aaagcagcgg atgcatattg gggaaaagat gcaaacggca agcaaatgac
    28201 ccgcgaagac gcctttaaaa aaattcacca acagcgtgat gagtttaata aacagaacga
    28261 tagcgaggcg ttcgcggtta aatatgacaa agaggtttat caaccggcga ttgcggcatg
    28321 tcataaacag agtgaagagt gttatgaagt gccgattcag cagaaacgag atttcgatat
    28381 caacgaacaa cggcgacaga cttttctgca atcacaaaaa ctcagccgta aattgcagga
    28441 tgactgggta acgctcgaaa aagggcaata tccgttaacg atgaaagttt cagaaataaa
    28501 cagtaaaaaa gtcgcgattt tgatgaaaat tgacgatatc aaccaagcta atgaacgctg
    28561 gaaaaaagat accgaacaac tgcggcgcaa tggggtgatt aagtaagttt gatatagtgc
    28621 ctttgccgga taatataaaa agttatccgg caaattattt tttacattag ccctagtagc
    28681 tgtggaatga acaaggatat ttgcggaatg taagtgatca tcatcaacgc tactagcaat
    28741 gcagcataaa atggcagcaa tggtttgatt aaatgttgaa tcttcacacc cgagatagaa
    28801 caaccaacaa atagcgcact acccaccggc ggtgttaaca gcccaataca aaggttggcc
    28861 accatcataa taccgaaatg tactgggtcc attcccagct cttgtgcaat cggcaagaag
    28921 atcggtgtaa agattagtac agccggtgtc atgtccataa agatgccgac aattaataag
    28981 acgatattaa tcagcagcaa tatgatcagt gggttatcag aaatccccat caatgcgtcg
    29041 ctgatcatat aaggaatatc agcattagtc attgcccagg acatgcccac ggaaaaaccg
    29101 atcaacagta atacaatcga agtggtaaca accgactcca gaatcagttt gggtaaatgg
    29161 cgccatttta cttcccggta aatcagcacc gagagaataa atgtgtagac aacggcaatt
    29221 gctgacgctt cagttgcggt aaaaatgccg cccagaatgc cgcccatcac aatgaacacc
    29281 agaagcaaac ttggtaaggc atccagaaac gctttacagg actgagcaaa ggttgctctt
    29341 tctgaaagcg ggtagccacg gcgtttagca ataatgccgc agactatcat taccgcaaga
    29401 cccattaaga tccctggcaa atatcccgcc ataaatagcg atgctacaga aacgccgcca
    29461 gctgtcaggg aaaacacaat taacacgttt gagggcggta tcagtaatcc agtaatacaa
    29521 gatgagacgt ttacagcggt agaaaatgcc ggatcataac cttgtttggt ctgaatggga
    29581 ttcagtgtgc caccaacggc agcagctgcc gcaacggctg aaccagaaat ggaaccaaac
    29641 atcatattcg ccaggacgtt cacatgacca agtgaaccag gaacccgacc aatcattacc
    29701 tgggcgaggt taatcaaacg tatcgcaata ccgccgctgt tcatcagcgt tccggcaaag
    29761 atgaaaaagg ggattgccaa tagggcaaag ttatccagtc cattagccag tcgctgcgaa
    29821 acagtgattg ccgcgatatc ccagggaaac atcaaaagca ttgaagctac agtggcaata
    29881 ccgattgaga acgaaatggg cataccaata aagaccaaca caaaaaaaca gccaaacagt
    29941 gtaatcgcga tataactatc cattaactgg cctcctgcgc atgttgtttc agttgttgaa
    30001 tgctttgatt aataaaacac aaggcataaa acatcatcac caatccactt aatggcagaa
    30061 tgatatatac gtatcccatc ggtatttgca tcgcggcaga tacctgtgat gttgccagcg
    30121 ttttatcaat taatttcagg ccaccagtca caatgaccga accggcaaaa cctaaaataa
    30181 gaacgttaat gactatacta agaagtaatt gcttgcgctt attcagggcg agagcaaata
    30241 aatcgataga gagatgtctc tgcgcgccga cggtatatgc agcacctaat aaccctaccc
    30301 agatcatcaa aaatcgtgcc agctcatcgg ttaatgtact tggctggtta agtacgtagc
    30361 ggctaaatac ttgccagact acgcatacaa ccagagcaag catgacggcg acagaaaacg
    30421 cggcaatgac gcggtctacc gcaagtttca ggcggttaaa tatcattgta agcatcctgg
    30481 aaaatcaggg ccagagactg gcccggaata ttattgagca gcgttgtcga acttctctaa
    30541 caaagcagct tgttttggat cttttttgaa gtcgtcaaac agaggctgaa ctgcggcacg
    30601 gaatggtgct ttatccactt ttacaatttc accccccata gctttcgcct gagcacgtgt
    30661 gtcagcgtca attttttccc acaatttttg ttgataagct tcggatttct tcgctgctgt
    30721 ttcgaggatt tgttgctgtt ctggggttaa tttattccat gttttggtgg agataaccaa
    30781 aaagtcagga attgaggcgt gttcatcttc agaaaaaact ttggcaattt caatatgtcg
    30841 agtctgcacc caggaaggca cgttattttc tgcgccatct actacacctt gctgcatcgc
    30901 agtataaact tcgccaaatg aaattggagt gggtgaacca cccatcagtt caatcatttt
    30961 gatggtggtc ggacttggct gaacacgaat tttcagccct ttaagatctt ctggcttagt
    31021 aatcggtttt ttcgcataga aactgcgtgt acccgctaca taagctgata aagcaaaaaa
    31081 gcctttgtct tttgttgagt ccataatttc tttaccaact tcaccaaaaa caactttgtt
    31141 gaagtgagcc tgatctttaa ataagaacgg caaattatag atagcataaa tattatcaaa
    31201 agactccaga tcactggcgg aacctttggt catatccaga gcgccatttt gcaacaactc
    31261 catagtttct cgcgcattac ccatctggct gctgggataa atacggatca ccatattacc
    31321 ttttgacatc tgttttacct ctttcgccag ctcttcaaaa ctctgatgta caacatgact
    31381 tctttccagg ttgtgtgcta actttaacgt cactttttct gcggcgttta cgccagtgct
    31441 aaccagagcc agcaaggatg cgcagactaa tgagcgggta aatgacctca ttttcataaa
    31501 aaaactccct gttgaaggat ggaaattggt tgatatctaa ttgtgcttat cagtgctttg
    31561 tgcgacaaat tggttaacct gtctgacaac ttgtctatgt ttatcacaac ttgaaataaa
    31621 gtcagaaatt aaatattaaa tttgtgcgcc aggttgtaca tgtttataat ca
//
LOCUS       DAPGOF010000044        31567 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.044, whole genome shotgun sequence.
ACCESSION   DAPGOF010000044 DAPGOF010000000
VERSION     DAPGOF010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31567
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.044"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            65..1447
                     /locus_tag="RHV13_001716"
     CDS             65..1447
                     /locus_tag="RHV13_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HDV1241258.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISIAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKNVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            1457..3775
                     /gene="yicI"
                     /locus_tag="RHV13_001717"
     CDS             1457..3775
                     /gene="yicI"
                     /locus_tag="RHV13_001717"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HDV1241259.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACTWYADKLNGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CHMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     EVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYAW
                     HEGTAFHLFNLQDGHETVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(3828..5537)
                     /locus_tag="RHV13_001718"
     CDS             complement(3828..5537)
                     /locus_tag="RHV13_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HDV1241260.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDIRRPSLA
                     LTANSSTVNIRELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSAE
                     LVQ"
     gene            complement(5658..7049)
                     /gene="xanP"
                     /locus_tag="RHV13_001719"
     CDS             complement(5658..7049)
                     /gene="xanP"
                     /locus_tag="RHV13_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HDV1241261.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            7329..8534
                     /gene="gltS"
                     /locus_tag="RHV13_001720"
     CDS             7329..8534
                     /gene="gltS"
                     /locus_tag="RHV13_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HDV1241262.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDISLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            complement(8703..10784)
                     /gene="recG"
                     /locus_tag="RHV13_001721"
     CDS             complement(8703..10784)
                     /gene="recG"
                     /locus_tag="RHV13_001721"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDV1241263.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNITFGGRRMMTCQISDGSGILTMRFFNFNAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIERDMALNVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIANGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(10790..11479)
                     /gene="trmH"
                     /locus_tag="RHV13_001722"
     CDS             complement(10790..11479)
                     /gene="trmH"
                     /locus_tag="RHV13_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HDV1241264.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITEEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(11486..13594)
                     /gene="spoT"
                     /locus_tag="RHV13_001723"
     CDS             complement(11486..13594)
                     /gene="spoT"
                     /locus_tag="RHV13_001723"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HDV1241265.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(13613..13888)
                     /gene="rpoZ"
                     /locus_tag="RHV13_001724"
     CDS             complement(13613..13888)
                     /gene="rpoZ"
                     /locus_tag="RHV13_001724"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDV1241266.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(13943..14566)
                     /gene="gmk"
                     /locus_tag="RHV13_001725"
     CDS             complement(13943..14566)
                     /gene="gmk"
                     /locus_tag="RHV13_001725"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDV1241267.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            14824..16505
                     /gene="ligB"
                     /locus_tag="RHV13_001726"
                     /pseudo
     CDS             14824..16505
                     /gene="ligB"
                     /locus_tag="RHV13_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
     gene            complement(16502..17119)
                     /locus_tag="RHV13_001727"
     CDS             complement(16502..17119)
                     /locus_tag="RHV13_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HDV1241268.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(17411..18233)
                     /gene="dinD"
                     /locus_tag="RHV13_001728"
                     /pseudo
     CDS             complement(17411..18233)
                     /gene="dinD"
                     /locus_tag="RHV13_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
     gene            complement(18455..19318)
                     /locus_tag="RHV13_001729"
     CDS             complement(18455..19318)
                     /locus_tag="RHV13_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDV1241269.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRSHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            19445..20161
                     /gene="rph"
                     /locus_tag="RHV13_001730"
     CDS             19445..20161
                     /gene="rph"
                     /locus_tag="RHV13_001730"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDV1241270.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALNKLVASGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            20227..20868
                     /gene="pyrE"
                     /locus_tag="RHV13_001731"
     CDS             20227..20868
                     /gene="pyrE"
                     /locus_tag="RHV13_001731"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDV1241271.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(20905..21501)
                     /gene="slmA"
                     /locus_tag="RHV13_001732"
     CDS             complement(20905..21501)
                     /gene="slmA"
                     /locus_tag="RHV13_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HDV1241272.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(21608..22066)
                     /gene="dut"
                     /locus_tag="RHV13_001733"
     CDS             complement(21608..22066)
                     /gene="dut"
                     /locus_tag="RHV13_001733"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDV1241273.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(22044..23264)
                     /gene="coaBC"
                     /locus_tag="RHV13_001734"
     CDS             complement(22044..23264)
                     /gene="coaBC"
                     /locus_tag="RHV13_001734"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HDV1241274.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDMICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            23436..24104
                     /gene="radC"
                     /locus_tag="RHV13_001735"
     CDS             23436..24104
                     /gene="radC"
                     /locus_tag="RHV13_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDV1241275.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            24321..24557
                     /gene="rpmB"
                     /locus_tag="RHV13_001736"
     CDS             24321..24557
                     /gene="rpmB"
                     /locus_tag="RHV13_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDV1241276.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            24578..24745
                     /gene="rpmG"
                     /locus_tag="RHV13_001737"
     CDS             24578..24745
                     /gene="rpmG"
                     /locus_tag="RHV13_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDV1241277.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            24843..25652
                     /gene="mutM"
                     /locus_tag="RHV13_001738"
     CDS             24843..25652
                     /gene="mutM"
                     /locus_tag="RHV13_001738"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HDV1241278.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(25691..26170)
                     /gene="coaD"
                     /locus_tag="RHV13_001739"
     CDS             complement(25691..26170)
                     /gene="coaD"
                     /locus_tag="RHV13_001739"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDV1241279.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVELAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(26178..27455)
                     /gene="waaA"
                     /locus_tag="RHV13_001740"
     CDS             complement(26178..27455)
                     /gene="waaA"
                     /locus_tag="RHV13_001740"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HDV1241280.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSSSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            27868..28926
                     /gene="rfaQ"
                     /locus_tag="RHV13_001741"
     CDS             27868..28926
                     /gene="rfaQ"
                     /locus_tag="RHV13_001741"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HDV1241281.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            28923..30047
                     /locus_tag="RHV13_001742"
     CDS             28923..30047
                     /locus_tag="RHV13_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDV1241282.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVEANCGEAIVEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            30040..30837
                     /gene="rfaP"
                     /locus_tag="RHV13_001743"
     CDS             30040..30837
                     /gene="rfaP"
                     /locus_tag="RHV13_001743"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HDV1241283.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKSMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            30853..>31567
                     /locus_tag="RHV13_001744"
     CDS             30853..>31567
                     /locus_tag="RHV13_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,3-galactosyltransferase"
                     /protein_id="HDV1241284.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVNFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRAQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAIVSRLTY
                     MDQDILNLILLGKVKFIDAK"
BASE COUNT         7458 a         7837 c         8440 g         7832 t
ORIGIN      
        1 cacagggatg gtttacaaaa tgcgttatcg gtctcagcac ccctatagca tcaaggaaaa
       61 gcagatgaag agtgaagtgt tgtccgttaa agagaaaatt ggttatggca tgggagacgc
      121 cgccagccac attattttcg ataacgtaat gttatatatg atgttctttt ataccgatat
      181 ttttggcatt cctgccggat ttgtcggaac catgtttttg gtcgctcgtg cactggatgc
      241 gatttccgat ccttgcatgg ggttgttggc cgatcgaacg cgctctcgct ggggtaaatt
      301 tcgtccgtgg gtactgtttg gcgcactgcc attcgggatc gtctgtgtac tggcctatag
      361 cacgccagat ctcagtatga acggcaaaat gatctatgca gcaattactt acaccctact
      421 taccttactt tataccgtcg tcaatatccc ttactgcgca ttgggtggtg taatcaccaa
      481 tgacccgact cagcgtatct cgctgcaatc ctggcgtttt gtgctggcga ccgcgggagg
      541 catgctttct acggttctga tgatgccact ggttaattta attggtggtg ataataaacc
      601 actcggtttc cagggcggta tcgcggtcct ttctgtggtg gcattcatga tgctggcatt
      661 ctgttttttc accaccaaag aacgcgttga agcaccacct acaacgacgt ctatgcggga
      721 agatttacgt gatatctggc aaaacgacca gtggcggatt gtcggtttac taaccatttt
      781 caatatcctg gcggtgtgcg tacgcggtgg ggcgatgatg tattacgtca catggatttt
      841 gggtacgccg gaagtgtttg tcgcttttct caccacttat tgcgtgggta acctgattgg
      901 ttccgcactg gcgaaaccgc tgaccgactg gaaatgtaaa gtcactatct tctggtggac
      961 gaacgccctg ctggcagtga ttagcatcgc gatgttcttt gttcctatgc aggccagtat
     1021 cactatgttt gtcttcatct tcgtgattgg tgtgttgcat caactggtga cacctatcca
     1081 gtgggtaatg atgtccgata ccgttgacta cggcgagtgg tgcaatggta aacgcctgac
     1141 cgggatcagt tttgctggca cgctgtttgt gctcaaactg gggttggcct tcggcggcgc
     1201 tcttatcggc tggatgctgg cttatggcgg atatgatgcg gcagaaaaag cgcagaacag
     1261 cgccacgatt agcatcatta ttgcgctatt cacgattgtt ccggcgatct gttatttgct
     1321 gagcgcgatt atcgctaaac gctactactc actcacgacg cacaatctga aaaacgttat
     1381 ggaacagctg gcccagggca aacgccgttg ccagcaacaa ttcacctctc aagaagtgca
     1441 gaactaagga acggcaatga aaattagcga tggaaactgg ttgattcaac ctggcctcaa
     1501 tttgattcac ccgcttcagg tgttcgaggt tgaacagcag ggtaatgaaa tggtggtcta
     1561 tgccgccccc cgtgatgtac gtgaacgtac ctggcagctt gacacgcctt tatttacgct
     1621 gcgctttttc tccccacagg aaggtattgt cggggtgcgg attgagcatt ttcagggggc
     1681 gctgaataac ggccctcatt atccgctcaa tattttgcag gacgtgaagg tcacaatcga
     1741 aaacacagaa cgttatgctg agtttaaaag tggcaactta agcgcgcgtg tcagcaaagg
     1801 tgagttctgg tcactcgatt ttctgcgcaa cggtgaacgt attaccggta gtcaggtgaa
     1861 aaataatggc tacgtgcagg acacgaataa tcaacgaaat tatatgtttg agcggcttga
     1921 tcttggcgtt ggcgaaacag tttatggtct gggagagcgc tttactgccc tggtgcgcaa
     1981 tggtcagacg gtagaaacct ggaaccggga cggcggcaca agtactgaac aggcgtataa
     2041 aaatatcccg ttctacatga ctaaccgtgg ttatggggta ctggtcaatc acccccagtg
     2101 tgtctctttt gaagtgggat cggagaaagt ctccaaagtg cagttcagcg ttgagagcga
     2161 atatctcgaa tactttgtta tcgacggtcc gacgccgaaa gcggtacttg atcgttatac
     2221 ccgttttact ggtcgtccgg cgctgccgcc cgcatggtcc ttcggcctgt ggctaaccac
     2281 ttcatttacc accaactacg acgaagcgac ggtaaacagc tttatcgatg gtatggcgga
     2341 acgcaatctg ccgctgcatg ttttccactt tgactgtttc tggatgaaag ccttccagtg
     2401 gtgcgatttt gagtgggacc cgctgacttt cccggacccg gaagggatga tccgccgcct
     2461 gaaagcgaaa gggctgaaaa tctgcgtctg gattaacccc tatattgggc aaaaatcccc
     2521 cgtctttaaa gagttacaag agaaaggtta tttactcaaa cgcccggacg gttcgctgtg
     2581 gcagtgggat aaatggcagc caggtctggc gatttatgac tttaccaatc cggatgcctg
     2641 cacatggtac gccgacaaac tgaacggtct ggtcgcgatg ggcgttgatt gctttaagac
     2701 cgactttggc gaacgtatcc caaccgatgt tcagtggttt gacggttccg atccgcagaa
     2761 aatgcataac cattatgcgt acatctacaa cgaactggtg tggaacgtgc tcaaggacac
     2821 cgttggtgag gaagaagctg tcttgtttgc ccgctcggcc tccgtcggtg cgcagaaatt
     2881 cccggtacac tggggtggcg actgttacgc taactacgaa tcaatggcgg aaagcctgcg
     2941 cggtggtttg tctattggcc tttccggttt tggtttctgg agccatgata tcggtggatt
     3001 tgagaacacc gcgccagcgc atgtttacaa acgctggtgc gcgtttggtt tgctctccag
     3061 ccatagccgt ttacacggta gcaaatctta tcgtgtgccg tgggcctatg atgatgagtc
     3121 ctgtgatgtg gtgcgcttct tcacgcaact gaaatgccac atgatgccgt atctgtatcg
     3181 tgaagctgcg cgtgcgaacg cgcggggtac gccgatgatg cgggccatga tgatggagtt
     3241 cccggacgat ccggcttgtg attaccttga ccgtcaatac atgctaggcg acaacgtgat
     3301 ggttgctccg gtgttcactg aggcgggcga ggtgcagttc tacttgccgg aaggtcgctg
     3361 gacacacctg tggcacaacg atgagctcga cggtagtcgc tggcataaac agcagcacgg
     3421 cttcctgagt ctgcccgttt atgtgcgtga taacacccta ctggcgctgg gcaacaacga
     3481 tcaacgtccc gattacgcgt ggcacgaagg cacggcattc cacctcttta atctgcaaga
     3541 cgggcatgaa accgtctgtg aagtgcccgc tgctgatggt tcggtgatct ttactttaaa
     3601 agcagcacgt actggcaaca cgattactgt gactggtgcg ggcgaggcga agaactggac
     3661 gctgtgcttg cgcaatgttg taaaagtaaa tggtctgcaa ggcggttcgc aggctgaaag
     3721 tgagcagggg ctggtggtga cgcctcaagg gaatgcgctg acaattacgt tgtaatctat
     3781 tgatggaatg ccgggtacgt ctgagtcgta cccggcgaga ttgatgttta ctgcaccagc
     3841 tctgccgacg taacttcacc gctggaaacg acgcccgcat tcatggtttg agtcacttgc
     3901 tgctttgcgg cgttcacggc atgcaattgc ccgctaaccg taggtttcag cgggacattg
     3961 gcctgaatat cgccactggc ggtaagctga atattgccgt cgccagtcaa cggtaattca
     4021 ggccatcccc actgttgcaa aatattcacc ggcacaccgc gtccattcag gctgatatgg
     4081 gtctgacgtt gtggcgtttg tgaaacactg gcggtggctt ccagaatgcc tttttcagta
     4141 aatgcactca gttcgcggat attcaccgtg ctgctgttgg cggttagcgc cagcgatggg
     4201 cgacgaatat caacacgatt aaacgtcgcg gcggcggcat tcagattcgc cgagccactc
     4261 cagacgcccc atttatgatc ggtaaccagc gtcagattgg caccgtaacc atcgagcgcg
     4321 gtgagctgcc acgggaagtc agggtcgata tcaatgatca gattgcggct ggcgctaaat
     4381 ctcttcagtt gcaggctgtt taaccagccg ggtgtcgttt ccatccacaa ctgttgccag
     4441 tttttcggca aggtatattc cagcccggca attgccgcat catcaaggat caacgttttc
     4501 ccgtcacgca gccagttccc tgacgttctg accatgcccc cttcccagcg gctggtgaac
     4561 tggcgcagcg ctacgccctg cggggaaaat tccgcgttta taatcgggtc aaataaatgc
     4621 agcgaaccat aaatgaactc gctagcgttc atcgacagtt tgccttcctg tgtctgccag
     4681 tcatctttac tgaaggtcat gttgcgcaag ctgagatcga ggtcggtcac cgcccagtcc
     4741 ggaccttgca gacgagcatc gatcacttcc aggcgaccaa tctgcaacga cgggacagag
     4801 cgtaatggcg caaagaagtc ggttagcgat ttttcgcttt gtagacggat atccgccatg
     4861 cgcagatttt ccacttgcca gctgccgtcg gcgttacgct gcgcgtttcc ggttaatgtc
     4921 ccgcgcgcga tgtcggcacc caggttagtc agcgtaacgc gatcgttatc aatactgcct
     4981 tcaatcagta cattggtggc aggaacatca ttgagcgaaa gcgatccggc actaaactga
     5041 atctgcgcct tcgtacccag cactttaccg gcttctggtg accacggaac cacgccgcca
     5101 tttacccgct gcgcgctcag tttccattcg ctattcgggc tattaaacgc catatcacgc
     5161 agttgcagac gatcggcttt gaacggtagc ggcgcggtct ggtcggtgag attcagcgtc
     5221 ccgttttcca gcaggatggt atcgacatgg cgtggttcgg tcagttgccg actacttagc
     5281 gcaatgtcga cactttttgc caccagggtc gcgggctggc catcacggcc aaacgtgacg
     5341 ttctccagca cgatatgaga tggcgcggaa aaacggtgat ccatcgcccc gaaggccaga
     5401 tgatagtcgc tattctcgga aacccatgcg ctgatatgtt ctgctcccca gcgggtttgc
     5461 agaagaaaat agaggccagc gatcaccact aacagagcga tgagaatgta gagaagcagc
     5521 ttcccaataa atttcatggt cttccatccc gtgaaatgca cataagggag ttatgcacga
     5581 tttacgcgca atcctcaagg cgggaatggt gaaagaggtg agactgcggc ggtaattatc
     5641 ttaccgccgc cagtgaatta ctgtttttct ggtgggaaaa tcagattcag cacgatggca
     5701 gtaataccgc ccgcggcgat cccggaggag agcaggtttt tcagccattc aggggcaaac
     5761 tgcaaaatca gcggctgctg agacacgccc agaccaaccg ccagcgacag ggcgataatc
     5821 agaatcgccc gacggttcag tggctcacga gaaacgatac gcacaccgga ggcggcgatg
     5881 gtgccaaaca ttacaagcgt tgcgccgccc agaaccggtt ctggaatgtg ttgtacaaaa
     5941 ccgctcactg ccgggaacag acccagcacg atcaacatca gcgcgacgac aaaaccgaca
     6001 tagcggctgg caacaccggt caactggatc actccgttgt tctgcccgaa gcaggagttc
     6061 gggaaggtgt taaacaccgc cgaaacaaac gagttcaggc cgtttgccag cacgccgcct
     6121 ttcaggcgtt tcatgtacag cggaccggac actggctgtt cggaaacgtc agaggtcgcc
     6181 gtgatatcgc caatggtttc cagcgaagtg atcataaaga ccagcatcag cggcaacagc
     6241 agactccatt caatgccaag accgtaatag agcggcgttg gcaccataat cagttcttgc
     6301 gtcatcggtt cgttgctttc tggcaacatg cccataaacc acgccagcgc atatccggcc
     6361 gccatcgcaa tcaccagtga ggccacgcgt aagtaagggt tacgttgacg gttaagcagg
     6421 ataattaagg ctaagaccac gcctgccagc agcagatttt tcggtgcgcc gaaggtgtta
     6481 tcgctcatgg ctgcgtaacc gccgccaatt gaggtcagcc ccacctgaat tagcgacaga
     6541 ccgataatca tcaccacaac gccagaaacc agcggcgtaa taatgcggcg cgccagatgc
     6601 agaacacggg agatcaccat ctcggtgcaa cttgccagca tcaacgtgcc gaacaaagcc
     6661 gccatcatgg taggaacatc agcaccaccg gttttcagcg cggtaccgcc cataatcagc
     6721 ggggcaacaa agttgaagct ggtgccctga atagacaaca gcccggagcc aaccggaccc
     6781 caggccttaa tttgaataat cgatgccaca ccggaggcaa acagcgacat actaataatg
     6841 tgttgcgtgt cttgtgccgg taaacccagc gcctggcaga ttaatagcgc tggcgtgatc
     6901 accgcaacga acatcgccag caaatgctga caggctgcaa acagggtttg aggaagcggc
     6961 ggacgatctt caagacggta aatcagttcg ctgttttgag tctgcgcaac cggttgcgca
     7021 ttttctgact cgagggtgga aacagacatc gaaaacaatc ccgtggtagc aaagcgggca
     7081 ttttagctga ccgaaagaga aaagcaaacg tttgccacat cctcatctaa atatttaaat
     7141 gagataaatg cactttttta tataactttt ggtatttttc tgcttaaaat ccatgccatt
     7201 tgttcattaa cagctgagaa tttgacccag gaaagcatga ctcgataccc gtcatatttc
     7261 atgaagttgc cactgcgcga tgaagtatga cgagtatgaa agagtgatgc ggatacaaag
     7321 gagtaactat gtttcatctc gatactttag caacgcttgt tgccgcaacg ctgacgttgc
     7381 tgctcgggcg taagttggtc cattccgtct cctttttgaa gaaatacacc ataccggaac
     7441 ctgttgcggg tggtttgttg gtggcgctgg cgctactggt actgaaaaaa agcatgggct
     7501 gggaagtcaa ctttgatata tccctgcgcg atccgttaat gctggctttc ttcgccacca
     7561 ttggcctgaa cgccaacatt gccagtttgc gtgccggtgg gcgtgtggtt ggcatcttct
     7621 tgattgtggt tgttggtctg ttggtgatgc aaaatgccat tggcattggg atggctagcc
     7681 tgttagggct tgatccgctg atggggctgt tggccggttc tattacgctt tccggcggtc
     7741 acggtacggg agctgcatgg agtaaattgt tcattgaacg ttatggcttc accaatgcga
     7801 cggaagtggc tatggcctgt gcaacgttcg gtttggtgct gggcggcttg attggcggtc
     7861 cggtagcgcg ctatctggtg aaacactcca ccacgccgaa cggtattccg gatgaccagg
     7921 aagtcccgac ggcgtttgaa aagccggatg taggccgcat gatcacctcg ttggtgctga
     7981 ttgaaactat cgcgctgatt gctatctgcc tgacggtggg gaaaattgtt gcgcaacttt
     8041 tggctggcac tgcttttgaa ctgccgacct tcgtctgtgt actgtttgtt ggcgtgattc
     8101 tgagcaacgg tctgtcaata atgggctttt accgcgtctt tgagcgtgcg gtatccgtgc
     8161 tgggtaacgt aagcttgtcg ttgttcctgg cgatggcgtt gatggggctg aaactgtggg
     8221 agctggcttc gctggcgctg ccgatgcttg cgattctggt ggtacagacc atcttcatgg
     8281 cgttgtatgc catcttcgtt acctggcgca tgatgggcaa aaactacgat gcggcagtgc
     8341 tggctgcggg tcactgtggt tttggcctcg gtgcaacgcc aacggcaatc gccaacatgc
     8401 aggcgatcac tgaacgtttt ggcccgtcgc acatggcgtt tttggtggtg ccgatggtcg
     8461 gtgcgttctt tatcgatatc gtcaatgcgc tggtgattaa gctgtatttg atgttgccga
     8521 tttttgccgg ttaaccgatg aagcggcggt agaagtgccg ccgcaacaaa gacaaatgcc
     8581 tgatacgctt cgcttatcaa gcctgcgtat tgattaataa tttattgaat ttgtagggtg
     8641 atgaatcgca tccggcagga aggtagggta acctgaaatg gcggtcttct cactgccgcc
     8701 ttttacgcat tcgagtaacg ttccgtctcc ggcatccagc gttctatcag ggcttttgcc
     8761 tgttgtgggt aacgttcgtg aatatggcgt gccaggcgct gaacttccgg gatcatcgcc
     8821 tgatcgcgca gtaaatctgc cactttaaat tcagcattac ccgtctgacg agtgcctaac
     8881 aattcgccag ggccgcgaat ctccagatct ttttgtgcaa tcacaaaccc gtcgttgctg
     8941 tcgcgcagca cttgcaggcg aatttgcgcc gttttggaaa gcggcgtttt gtagagcagc
     9001 acacagtgag aagccactgc gccacgacct acacgcccgc gtagctggtg caactgcgcc
     9061 agacccagac gctccgggtt ttcgataatc atcagactgg cgttaggcac atcaacgccg
     9121 acttcaataa cggttgtggc aaccagcagg tgtagctcac cttgtttaaa cgacgccatc
     9181 accgcctgtt tctcggcagg tttcatccgc ccgtgtacca ggccaacatt caactctggt
     9241 agcgccagtt tcaactcttc ccaggtagct tccgccgcct gcgcttccag caattccgac
     9301 tcttcaatca acgtacaaac ccagtacgcc tgacgacctt cagtcatgca ggcgtggcgc
     9361 acgcggtcaa tgatgtcggt acggcgagta tcaggaatag cgaccgtagt cactggcgtg
     9421 cggcctggcg gcagttcatc aatgaccgag gtgtcgagat cggcatacgc agtcatcgcc
     9481 agcgtgcggg ggatcggcgt ggcggtcatg atcaactgat gcggatggaa gccctgctgc
     9541 tggcctttct cccacaatgc cagacgctga tgcacgccaa aacgatgctg ttcgtcgata
     9601 atcaccagcg ccagaccgtt aaactgcacc tgttcctgga agatggcgtg cgtaccaaca
     9661 atcatctgca cctgaccgtt ggcgatggct tcctgctgag acagtcgcgc tttacctttc
     9721 tgcttaccgg cgagccagcc cacttcgata ccgagcggtg caaaccagtt gcggaagtta
     9781 ttggcgtgct gttcggcgag taattcggtt ggtgccatca atgccacctg tttgccgtgg
     9841 gcaatcgcac gcagcgcggc gagggcagcg accagcgttt taccggaacc tacatcgccc
     9901 tgtaccaggc gcatcatcgg cacattcagc gccatatcgc gctcgatctc cgccaccacg
     9961 cgagcctgag cgcccgttgg cttgaacggt aaggcggcaa gaagtttatt tttcagcgtg
    10021 tcattggcgc tcagcggctg ggcatggaaa cgttgcgctc cggcgcgtaa ggctaacatg
    10081 ctgaggttat gcgccagcag ttcttccaga atcagacgac gttgcgccgg atgctgcccg
    10141 gtttccagat cgctaagttg cagcgtcggc ggcgggcggt gcaaagtgcg caacgcttcc
    10201 ggtagcgtca tcattccttg tgacagttcc ggcggcagga gttcttcaat ggcgcaggtg
    10261 tcgagcagat ccagcgcctg gtcggttaat ttacgcagcg tggcctgctt tacgccttcg
    10321 gttgttggat aaaccggcgt gagcgtttcc tgcaactctg gcgtactgag atcgccctgc
    10381 acgcggtatt ccgggtggat catctccgca ccatatttac cgcgctttgc ttctccatag
    10441 gccagcacgc ggcggcccgt tgccaggcta tttttcattg ccgcgttgaa gttgaaaaag
    10501 cgcatggtga gaatgccgga accgtcgctg atctggcagg tcatcatccg gcggccgccg
    10561 aaggtgatat tgcaattcag cacttcgcct tccaccgtgg cataaacgcc cggtagtagt
    10621 tctccgatgg ggtagagatg ggtgcgatct tcgtagcgca ggggaaggtg taacagtaga
    10681 tcctgcacgg tatgcaggtt gattttcgcc agtttgttac taagtgctgc gccaacgccc
    10741 gttagggaac tgagcgggac agcatctaac aggcgacctt tcatggcact taccctgcag
    10801 cctgcatagt agcccaccag tcggcatcag cttcgatctc gccttgctga ttgacgtggg
    10861 gataaggcag gcctttgcgt tttgcgactt tcgccagcac cggatagccg ccttcaaaca
    10921 acaggcgttg ttgctctgct tccggcaaca tgctgttttc acgcaggtac atgcctgcat
    10981 tttgccgctg acgctgggct tcgtaaagaa tgagggctga ggcaacggaa acattcagcg
    11041 actgcaccat gccgatcatc ggaatgatga tgtcctgatc cgccaaggcc aacgcttcct
    11101 cagtgatgcc cgttttctcc tgacccatca aaatgcaggt cgggcgtgtg taatcaattt
    11161 cgcggaaatc gacagcgtta tcagaaagat gggttgccag aatctgcatg ccctggcctt
    11221 tcagatgagc gacggcatcg ccaatggtgc ggtgcgtttt cacctgcacc cagctgttac
    11281 tacccgccgc cgccgaagcc atggtgcgca tccggctgcc aggccagaca gcatgaactt
    11341 catgtacgcc aacggcatct gcggtacgaa taatcgcaga aacgttatga ggtttgtgga
    11401 cctgctccat gcagacggtc agatcaggct gccgcctggc gagcatttcg cagatgcgtg
    11461 cataacgtgt tgggttcata aaacattaat ttcggtttcg ggtgacttta atcacgtctg
    11521 gcatcacgcg gattttgcgc atgatattcg ccagatgcac acggtcacga gcggtcaaac
    11581 gaataaaggc gctgtagacg cgaccatctt tctcttccgt attcaaactt tgaatattcg
    11641 aagtcgtggt gttaattgcc gccgtcaggt ttgccagcgc gccctgatga ttgaacatct
    11701 ccaccttgat ttcggtgatg aactcctgcg ccgtctcttt atcccattcc acagccataa
    11761 acttctctgg ctctttctgg tagccacgga tgttacggca ggattcatgg tggatcacca
    11821 gacctttacc ggggctgacg tgggcgataa tcgggtcgcc aggaataggg cggcagcatt
    11881 tcgcaaaggt gatcagcacg ccatcggcac ctttaatggg cagatgtccg tgactttggg
    11941 ttgccggtgg aatggaggcg tccccatgtt gcagattttt cgcgaccacc acgctcattg
    12001 cgttaccaag tccgatttct gccagcagat cgtcaagcgt tgccagcttc atacgatcca
    12061 gctcgcgctg aatattttcc tgcgggattt cattgagctt acggctgcca cccaacgcat
    12121 ggttgagcag acgacggccc agactaacgg aatcatcacg cttgaggttt ttcagcaact
    12181 gacgaatttt ggcgcgcgct ttcgagctga cgacaaagtt cagccacgcg gcattcgggc
    12241 gagcgcccgg tgcggtaatg atttcaacgg tttgaccgct agtaagcggc tgcgacagcg
    12301 ggtaaggctg gcggtcgacg cgtgcgccca cgcaggcatg accgatatcg gtatgcactg
    12361 cataagcgaa gtcgacgggc gttgcaccgg caggcagctc gacaatgcgc ccttccggtg
    12421 tgaaaacgta aatctcatcc gggaagagat cggatttaac gctctcgata aattcaaacg
    12481 agctaccggc actctgttgc agctccagca ggctttgcat ccagcgctgg gcgcggattt
    12541 gtgcggtagt actggtttcg ccgtgctctt tataagccca gtgcgcggca acacccatct
    12601 ccgccatctg gtccatatct tcggtacgga tctggacctc aaccggcaca ccgtgcgggc
    12661 cgatcatcga ggtgtgcaaa gactgatagc cgttcgcttt tggaatggcg atatagtctt
    12721 tcacgcggcc cggacgcggc ttgtacaggc tgtgcatctg gcccagcacg cgataacagg
    12781 tgtcagaatc attgacgatc acgcggaaag cgtagatgtc catgattgag tgaaaacgct
    12841 gctctttgag caccattttg cagtaaatcg aataaagatg cttttcgcga ccactgacgc
    12901 ggcacggtat tcccgcttcc tgcaaacgcc cttcgatttc agaaagaatc ttctggatca
    12961 tctctttacg gttgccgcgc gcggctttca ccacttcttt gattacgcga taacggttgg
    13021 gatacagcgc ctcaaaaccc agctcttcga gttcggtttt aatgtggtgg atacctaaac
    13081 ggtgcgccag cgggctgtaa atttcgagag tttcacgggc gatgcggcga cgtttgtccg
    13141 ggcgaagtga gcccagcgtg cgcatgttgt gggtacggtc ggcaagtttg atgaggatga
    13201 cgcggatatc ctgcaccatc gccataatca tcttgcgaaa gttttcggcc tgcgcctctt
    13261 tcttatcgcg gaacttgagt ttatcaagtt tcgacacccc ctctaccagc tcggcgacgc
    13321 ttttaccaaa aagctgttcc atatcctggt aggtggcggg agtatcttca atcacgtcat
    13381 gcagcagcgc cgccatcagc gtttcatagt cgagtttcat ctcggccaga atgcaggcaa
    13441 ccgctaccgg gtgcgtgata tagggttcac cgcttgaacg tgtttgcccc tcgtgagcat
    13501 cacgtgcaac gagatacgcc tgccgcagac gcttgatttg gtcttccggc aggtaggttt
    13561 gaatcagttg attcaggctt tcaaacagat acaagggcga cccgctttgt gattaacgac
    13621 gaccttcagc aatagcggta acggcttgta attcagcggc ttcctgctct tgctgttcct
    13681 ggcgttcgcg aacgtcgagg atctggttgt tgatcagacc ttcttcgatt tcgcgcagcg
    13741 cgattacagt ggttttatcg ttttcttccg gtaccagcgg atcctttccg cctacctgca
    13801 tctgacgagc gcgacgcgcg gcgaccagta ccaggtcaaa acggttacca attttctcta
    13861 cagcgtcctg aacagttacg cgtgccatac ttaaaaagct ccacaggtga agaaatgact
    13921 gggcatgata ctgaaatcag gttcagtctg ccaacaattt gctgattaaa gcgtcatgac
    13981 gctgcttttg gcggctcatg cgcagacgtt cggcgcgaat aatggtcttc aaatcggtca
    14041 acgcggtatc gaagtcatca ttcacaatca gataatcata ttcggcgtaa tggctcattt
    14101 ctgcaacagc ttgcgccata cgctttgcaa tgacctcttc gctgtcctga ccgcgaccgc
    14161 gtagacggcg gtccagttca attttggacg gcggtaaaat aaagatactc cgcgcgtgcg
    14221 gcatcttctg gcgaatttgc tgcgcgccct gccagtcgat atcgagaaaa acatcgacac
    14281 cggtcgccag tacttgctca atggcctcac gcgaagtgcc atagtaatta ccaaaaactt
    14341 ctgcgtgttc gaggaacgca tctctgctaa tcatttcttt aaattcatca tgattaacaa
    14401 agaaataatg ttcaccgtgg acttcaccag gacgcggttg gcgtgtggtg tgtgaaacag
    14461 aaacctgggt gtcatacaac ggttgggttt ttaataaagc ctgaatcagg ctggatttac
    14521 ccgcgccact gggggcagaa acaatataaa gcgtgccttg agccatgagt atctttcgta
    14581 tgtgattagc gaaataaagc ctacatacga gcttattata cacggcgcgg ccatgtgacg
    14641 tagcccttgc tcacactttt tgcgcaaagt tgttgcgttt tgcgtagctg tttccgcatt
    14701 tgcctgtgat ttactgcaat aaagacgatt ctcaggaagc gtcctcgcag actccgtttt
    14761 tgccactcgg ctttgccttc atcacgttgt atatctgcct gactatctac agcggaggtg
    14821 atgatgaaag tatggatggc gatattaata agtatcttgt gctggcaatc atctgtgtgg
    14881 gcggtctgtc cggcctggtc gccagccaga gcacaggaag aaatttcccg cctgcaacag
    14941 caaataaaac agtgggacga tgactactgg aagaaggaaa aagtgaggtg gaagacggtg
    15001 tttacgatca gttaagcgcc cgtcttacgc agtggcaacg ctgctttggg agcgagcccc
    15061 gcgacgtcat gatgccgcct ttaaatggtg cggttatgca tccggttgcc catacgggtg
    15121 tacgcaaaat ggtggataaa aacgcattaa gtctgtggat gcgagaacgt agcgatcttt
    15181 gggtgcagcc aaaagttgat ggcgttgcgg taaccctggt ttatcgggac gggaaactga
    15241 acaaagcaat cagtcgcggt aatggcctga aaggcgagga ctggacgcag aaagttagct
    15301 taatttccgc tgtgccgcaa accgttagcg ggcctttagc caacagtacg cttcaggggg
    15361 aaatatttct ccagcgcgag gggcatatcc aacaacaaat ggggggaata aatgcccgcg
    15421 caaaagttgc tggcctgatg aagcgccagg ggaatagcga cacgctaaac tcactggctg
    15481 tttttgtctg ggcatggccg gatggaccgc agttaatgac cgatcgttta aaggagctgg
    15541 ctaccgcagg ttttactcta acgcagaggt atacccgtgc ggttaaaaat gctgatgagg
    15601 ttgcgcgcgt acgcaatgag tggtggaaag cggaattacc cttcgtcacc gatggcgtag
    15661 ttgtacgagc ggcgaaagag ccagaatccc gccattggct accgggccag gcagagtggc
    15721 tggtggcctg gaaatatcaa cctgtagctc aggttgccga agtgaaggcg attcagtttg
    15781 cggtgggtaa gagcggtaaa atatcggtgg ttgcgtcact cgcacctgtc atgctggatg
    15841 ataaaaaagt ccagcgggtg aatattggtt ccgtcaggcg ctggcaggag tgggatattg
    15901 cgcctggtga tcagatcctc gtcagccttg ccggtcaggg tattcctcgc attgatgatg
    15961 tggtgtggcg cggtgcagaa cgtacaaaac cgacaccgcc agaaaaccgc tttaactcgt
    16021 tgacctgcta ctttgcttct gatgtttgtc aggaacagtt catttcacgc ttagtctggc
    16081 tggggtcaaa acaggttctg gggctggatg gcattggtga ggccggctgg cgcgcgctgc
    16141 atcagactca tcgctttgag catatctttt cctggctttt attaacgcca gagcaattac
    16201 agaacacgcc ggggatcgcg aaaagtaaaa gtgcgcagct atggcatcag tttaatctgg
    16261 ctcgtaagca gccttttact cgctgggtga tggcaatggg aataccgcta acccgggcgg
    16321 cgcttaatgc cagtgatgaa cggtcctggt cgcaactttt atttagcacg gagcagttct
    16381 ggcagcagct gccggggact ggatcggggc gcgccagaca ggttattgaa tggaaggaaa
    16441 atgcgcaaat caagaagctg ggcagttggc tggctgccca gcagatcaca ggttttgaac
    16501 cttagtgtcc ttcgtggctg tagtaaaaga ccggtaatcc cagcttaagg cgtagcgcca
    16561 gtaagcgggc aaaaaagccg aataccaggg tggagataat taccacatca tggttagaaa
    16621 catagtgttg cagcgcgatg tacagcacgg cggaggcaaa tgagacaccg gcatacaact
    16681 ctttctggaa taccagtggg atacgtttac agaacatatc gcgcaaaacg ccgccaaaca
    16741 cgccggtggt taccgccgcg acaacggcaa taattgggcc gtgccccata tccagtgcaa
    16801 cctgtgcgcc gatgatagaa aagaccacca gtccaagcgc atcgagcacc agaaacactt
    16861 tgcgcaggta aggcattaca ggagcaacga tagtggtgag tactgcggcg gtagcgacga
    16921 taatcacata ttccgggtgt ttgacccagc cgagcggata gtggcccagc agaatatcgc
    16981 gcactgaccc tccgccaatt gcggtggcgg tcgcaataat aattacgcca aatgtatcca
    17041 tgcgccgtcg tccggccgcc agcgcccccg tcatggcttc ggcagtaatg ccaaccaaat
    17101 acaaaatgtg taacagcatg gttctctccc taaaaatctg tctggcaggg tagcgatttg
    17161 tgcgcattgt cacgattgag attttttaag gtgaggagaa attgataact tgtagttatg
    17221 tgaaatcgct attttctgta gcggagatgc attcttctga cttcttgtct gagaaaatgc
    17281 ataagaaaaa ataagttaaa attgaggggt ggaaaagaaa ggtgcaatgt ttagccattg
    17341 tgttcaccgc acctggactt tggcttacgc atcgggtgtc tcaatcaaga aaaagagttt
    17401 ttagtttctg ctatttcttc tctgtaattt ttacactgtt tcccaactgc ttgatgctga
    17461 cctgcggggt cggcaactcc tcaggcatgg ttccgccaag ttcctgaatg gtttgtctca
    17521 ccttgcgacc cacgtcaaag tgtttggtat tagcttgctg ttttgaattc acgccatccc
    17581 gcttgagttt ttcttctgtt tgggtagctc gaaagagatt agccgccagt tctgttgaac
    17641 ccatatgatc caggatcttc tgactctttt tcagctcctt ccgctgataa atagctttct
    17701 gatctaatcc accatacagc cccggtaacc atgattctga aatatggcga aatcgatagc
    17761 tgctgctacc ccagcttgct gcgctgcctc aactaattgt ttgttatgtt ctttcaattc
    17821 atttctcaga aaaagacgct tttcgtcttc acgaagttgc ctgaaagctt catcgtcagc
    17881 cagctactgc cgtcgggtct ggatagcaaa ataagtttgt cctgccgcaa taaccggttt
    17941 cgcagggtcg ccgttttgta ctaccaggta acaggcatag cgggagagat gaacatcttc
    18001 aagttctcgt tgagccctga acctaataca accattttgg tggtttcaac gaaatggtca
    18061 gatgcagctt gattgctggc ttcgcacgct tgagtcgcgc gggtcagtac cttttgaaaa
    18121 ttgcaccagt cccgataatt cagtaacggt gccaattctc tcgctgacca aaattcttgc
    18181 ccttcagtgc cataatgccg tatttcttca aaaggttgat gatgttcgtt cattgcccac
    18241 tccatgtgat atcgattgca aagaaagtaa gtctataact gtatttatat acagttgtga
    18301 gtatctcacg ttctcagtgt gctgggcaaa aaaacatcgc aaaatctgga gcaggcacaa
    18361 aaaacagaat tgaaagttgc ggggaagaaa aatgccgctc tgtttgagtg ggcggcattt
    18421 gtgaaggctg aaaagtaaga attggcgtta cgagttattc aatattctga atctgctcgc
    18481 gcatctgctc aatcaacact ttcagctcaa tcgcggagtt tgtcacttcg gcattgatag
    18541 acttcgacgc aagagtgttc gactcgcggt tgaactcctg catcataaag tccagacgac
    18601 gaccaacggc ttcttttttc ttcagaatgt tgtaggtctc tttgacatgc gcttcgaggc
    18661 ggtccagttc ttcggcaacg tcaattcgtt gtgccagcag aaccaactcc tgctccagac
    18721 ggttgttttc caactgcacc tgagcatctt ccagcttcgc gaccaagcgt tcacgctgcc
    18781 attgcaggat ttccggcata tgggagcgga ctttgaccac ttcggcggtg acgccttcca
    18841 gacgctgctc gatcaatgct ttcagtgcct gaccttcggt ttcgcgcgcg acgataaagt
    18901 cgtccagcgt accatccaac gccgcgagaa tttcagcggc aatcgcgtcg agatcctgct
    18961 cctgggctgc catcacgccc ggccagcgta gaatatcaac cgggttgatt tccccttcgt
    19021 cactctgcat ttttacccag ttcgcggcag ttaccagctg tttagccagt ttttcattga
    19081 ggatcagctc accttgtgcg ctaacatctg gctcatagcg cagggtacat tccactttac
    19141 cgcgcgtcag gcgagaacga atacgctcgc gaacgacagg ttcaaggcta cggaactgct
    19201 ccggcagacg aaagtaagtt tccagataac gctggtttac cgagcgcatt tcccaggttg
    19261 cgctccccca ttcacccttg atttcacgcc gggcgtaggc ggtcatactg cggatcatag
    19321 acgttcctgt ttataaaagg agaggtggaa ggattatagc catcgatgcc ttgtcaggat
    19381 aggaataacc gccggaagtc cgtataatgc gcagccacat ttgtttcaag ccggagattt
    19441 caatatgcgt ccagcaggcc gtagcaataa tcaggtgcgt cccgttaccc tgactcgtaa
    19501 ctatacaaaa catgcagaag gctcggtgct ggtcgaattt ggcgatacca aagtattgtg
    19561 taccgcctct attgaagaag gcgtgcctcg cttcctgaaa ggtcagggcc agggctggat
    19621 caccgcagaa tacggcatgc tgccacgttc tacccacacc cgtaacgctc gtgaagcagc
    19681 gaaaggtaag cagggtggac gcacaatgga aatccagcgt ctgatcgccc gtgctcttcg
    19741 tgcggcagta gatttgaaag cgctgggtga gttcaccatt acgctggact gcgacgtgct
    19801 ccaggctgat ggcggcacgc gcaccgcttc gattaccggg gcatgcgttg cactggcaga
    19861 tgcgctgaat aagctggttg cgagcggcaa gctgaaaacc aacccgatga aagggatggt
    19921 agccgcggtt tctgtcggta ttgttaacgg cgaagcaatt tgcgatctgg aatacgttga
    19981 ggactctgcc gctgaaacgg acatgaacgt ggtgatgacc gaagacgggc gcattattga
    20041 agtacagggg acggcagaag gcgagccgtt cacccacgaa gagttactca ccttgttggc
    20101 gctggcccga gggggaatcg aatccattgt agcgacgcag aaggcggcgc tggcaaactg
    20161 atttttaagg cgactgatga gtcgcctttt ttttgtctgt agaaaagtaa gatgaggagc
    20221 gaaggcatga aaccatatca gcgccagttt attgaatttg cgcttggcaa acaggtatta
    20281 aagtttggcg agtttacgct gaaatccggg cgcaaaagcc cctatttctt caacgccggg
    20341 ctgtttaata ccgggcgcga cctggcactg ttaggccgtt tttacgctga agcgttagtg
    20401 gattctggca ttgagttcga tctgttgttt ggccctgctt acaaagggat cccgattgcc
    20461 accactaccg ccgtggcgct ggcggagcat catgaccttg acctgccgta ctgctttaac
    20521 cgcaaagaag caaaagacca cggtgaaggc ggcaacttgg ttggtagcgc gttacaaggg
    20581 cgtgtgatgc tggtggatga tgtgatcacc gccggaacgg cgattcgcga gtcgatggaa
    20641 attattcagg ccaatggcgc gacgcttgct ggcgtattga tttcgctcga tcgtcaggaa
    20701 cggggtcgcg gcgagatttc ggcgattcag gaagttgagc gtgattacaa ctgcaaggtg
    20761 atctctatca tcaccctgaa agacctgatt gcttacctgg aagagaagcc ggaaatggcg
    20821 gaacatctgg cggcggttaa ggcctatcgc gaagagttcg gcgtttaaag aaactcgccg
    20881 gatgaaaagt catccggcgt catattactg caactgcgcg gcaattagcg gccagcgggc
    20941 gtcaaaatca tccgtcgggc ggtatttaaa ctcgctgcgg acaaagcgtg acagcatacc
    21001 ttcacagaag gccaggatct ggcttgccag cagggtttca tcggtggtgt aaccttcacc
    21061 ctcacgcatt ctcttttcac gcaatacctg acgcagctgc gcttcaatac gctcgaaaag
    21121 ttggttgatg cgcccttgca ggcggtcttg ttcaaacatc agcgcatgcc cggtaaggat
    21181 gcgggtcagg ccaggattac gctcaccaaa accgaggagc aacaacacga tcagacgcag
    21241 gcgcgctgtg gtgtctttct catctttcag aatcaaattg atgcgagtaa tcaggctatc
    21301 ttcgataaac tcaatcaggc tatcgaacat gcgggtctta ctggggaagt ggcgatacag
    21361 tgccgcttcg gaaacgccga cagaggcggc cagttttgcc gtcgtgatac gttggcttcc
    21421 atcgctggat tccagcatca gcgccagaga ctgaagtatt tcttcgcgac ggttcctttt
    21481 cgcagtttgt ttttctgcca tgttacaaaa tacccctgaa aataagcact tgccaggcgg
    21541 cacccacgct atgaccgcaa acgaaatgtt tgcggctatg ttatgacgtt attcggatgc
    21601 gtatgtgtta ctgacgacca gagtgaccaa agccgccttc accgcggtcg gtggcgtcga
    21661 aatcttccac cagattaaat tcagcctgta ctaccggaac aaaaatcatc tgggcgatgc
    21721 gttcgccagg ttgaatggtg aagctgtcct gaccacggtt ccacacggaa atcatcaact
    21781 ggccctgata gtcggaatcg atcaatccta ccaggttacc aagcacgata ccgtgcttat
    21841 gtcccaatcc ggagcgcggc agcatcattg ccgccagtga aggatcggca atatgaatcg
    21901 ccagcccggt cggaaccagc gtagtgtcac ccggagccag ttctacggcg tcgtcgagac
    21961 aggcacgcag gtcaagtccg gcagagccag aggtggcata agtcgggagc ggaaattcct
    22021 tcccaacgcg cgggtccaga atcttaacgt cgattttttt catcataacg ggtcacgatc
    22081 tcgtcgagta ataattggcc aaggagctct ttgcgctcaa gcggtaagac tttatctccg
    22141 tcctgccaga aaaggtgtaa tgcgttgttg tcgctgttaa atccttgagt tggctgggaa
    22201 acatcgttcg cgcagatcat atcaaggttt ttacggatac gtttttgccg ggcgtattct
    22261 tccacattat ttgtttcggc ggcaaatcca acgacatagg gtcgatggtc ttttagtgcg
    22321 gcaacgcctg cgacgatatc ggggttttta accattttta ttgttaattc atcaccctgc
    22381 gtggcctgct ttttgatttt ttctggggcc acggtagctg cgcgataatc cgccacggcg
    22441 gcgcagccga taaaaatatt ttgctgctgt acagaagcat tcacggcggc ttccatttcc
    22501 agcgccgtca tcacatcaac acgtttaaca aacggtggcg tcggtagtga aaccggacct
    22561 gataccagcg tgacgttcgc gccacgacgg gcagcggcgg cggcgatagc aaaacccatc
    22621 ttgccggagc tgtgattaga gatataacgc accggatcga gcggttcacg cgtcgggccg
    22681 gcggtaatca taatgttcag atgtttcagg tcgttgacgg gcgaaaaatg cgccaccgcc
    22741 atatccacaa tggttaacgg atcgagcatt cgcccaggac cgatatcacc acaagcctga
    22801 ctgccactgt ctggccccca gatgagcaaa ccacgggaag caagcacctc taaattatgc
    22861 tgcgtggcag cggcacggta catctgctgg ttcatggcgg ggagcacggc tacaggcgca
    22921 ggtgtagcca gacaaatcgt cgataccagg tcattcgcca ttccggcagc aacacgggca
    22981 atcaaatctg ccgtggcagg ggcgagaatc actaaatcag cccatttacc cagctcaata
    23041 tggcccatag cggcttcggc tgccgggtca agcagactgt cggaaacggg atacccagaa
    23101 accgcctgca agctaagtgg ggtgataaag gcttttgccg cttcggtcat ggctacgcgg
    23161 acgtcggccc cgcgatcgcg caaacgacgc accagttcag gggttttata ggcagcaata
    23221 ccgccgctaa cgccgagaac gattttttta ccggccaggc tcatgatgat ttttcctgtt
    23281 gggtgacacc agaagttggc gattttatca caatactttt gttgtcgtgc cttcacctga
    23341 gaatcacttt gcgaggcgct ttccaggatt gaaaactggc cgtcgattta acggaacggc
    23401 tatgacagga tgcgagcacc acaaaggagg tgaaggtgaa aaacaatgca cagctgttga
    23461 tgccgcgcga aaaaatgctg aagtttggta ttagcgcctt aacggatgtc gagctgctgg
    23521 cgctatttct gcgtaccgga acgcgcggta aagatgtatt aaccctggca aaagagatgc
    23581 tggagaattt cggctctctt tatggcttgt taacctctga atatgaacaa tttagtggtg
    23641 ttcatggaat tggcgtggcg aaatttgccc agttaaaggg gattgctgaa ttggcgcggc
    23701 gttactacaa cgtgcggatg cgtgaagaaa gccctttact cagcccggag atgacgaggg
    23761 aatttttaca aagccagctc acgggtgagg agcgggagat ctttatggtg atctttctcg
    23821 actcacaaca ccgggttata acgcatagcc gtcttttttc cggcacgcta aaccatgttg
    23881 aagtccatcc tcgggaaatt atccgcgaag cgataaaaat aaacgcctcg gcgctgatcc
    23941 ttgcacataa tcacccttcg ggttgtgctg aacccagtaa agcggataaa ctcattactg
    24001 aacggataat aaagagttgt cagttcatgg atttacgcgt gctcgaccat atcgtgattg
    24061 ggcgtggaga gtatgtttct tttgccgaac gcggctggat ttaacccgct atgcgcgatc
    24121 cttagggatc tttgtctgtt cgggacttga gcacatcgct gagtcagcgt atactacgcc
    24181 acctttgaga atctcgggtt tggcatttgg gcctggcaat cgagagttca catagaactg
    24241 cgatgaccgg gctgtaaagc ctgacgaggc gccaataccc catacgaagc tcgagctaat
    24301 ttgatttttg gagaatagac atgtcccgag tctgccaagt tactggcaag cgtccggtga
    24361 ccggtaacaa ccgttcccac gcactgaacg cgactaaacg ccgtttcctg ccgaacctgc
    24421 actctcaccg tttctgggtt gagagcgaga agcgttttgt caccctgcgc gtatctgcta
    24481 aaggtatgcg tgtaatcgat aaaaaaggca tcgatacagt tctggctgaa ctgcgtgccc
    24541 gtggcgaaaa gtactaagta cttagaggaa ataaatcatg gctaaaggta ttcgtgagaa
    24601 aatcaagctg gtttcttctg ctggtactgg tcacttctat accactacga agaacaaacg
    24661 tactaagccg gaaaaactgg aactgaaaaa attcgatcca gttgttcgcc agcacgtgat
    24721 ctacaaagaa gcgaaaatca aataatttcc gctttgatgt aacaaaaaac ctcgctccgg
    24781 cggggttttt tgttatctgc ttgcccccat attgactgca tctgttcatt cctggagatg
    24841 ctatgcctga attacccgaa gttgaaacca gccgccgcgg catagaaccg catctcgttg
    24901 gtgcaaccat tcttcatgcg gtggtgcgca acggacgctt gcgctggccg gtttcagaag
    24961 agatctaccg attaagcgat caaccagtgc ttagcgtgca gcggcgggct aaatatctgc
    25021 tgctggagct gcctgagggc tggattatca ttcatttggg gatgtctggc agcctgcgca
    25081 tccttccaga agaacttccc cctgaaaagc atgaccatgt ggatttggtg atgagcaacg
    25141 gtaaagtgct gcgctacacc gatccgcgcc gctttggtgc ctggctgtgg accaaagagc
    25201 tggaagggca taatgtgctg gcccatcttg gaccggagcc gcttagcgac gatttcaacg
    25261 gtgagtacct gcatcagaag tgcgcgaaga aaaaaacggc gatcaaaccg tggctgatgg
    25321 ataacaagct ggtggtaggg gtagggaata tctatgccag cgaatcactg tttgcggcgg
    25381 ggatccatcc ggatcggctg gcgtcatcac tgtcgctggc agagtgtgaa ttgttagctc
    25441 gggtgattaa agcggtgttg ctgcgttcga ttgagcaggg tggtacaacg ctgaaagatt
    25501 ttctgcaaag tgatggtaaa ccgggctatt tcgcgcagga attgcaggtt tacgggcgaa
    25561 aaggcgagcc ctgtcgggtg tgcggtacgc cgattgtggc gactaaacat gcgcagcggg
    25621 caacgtttta ttgtcggcag tgccagaagt aattcatgcg cgccggatgg cataccatcc
    25681 ggcataaacg ctacgctaac ttcgccatca gcgcctgatg gacattctcc ggcaggaaat
    25741 gggtgacatc gccctgatgg cgcgccacct ctttcaccaa cgatgaagag ataaacgacc
    25801 actctttcga cggcatcaga aacacacttt ccagttccgg cattaagtgg cgattcatat
    25861 gcgccagctg catttcatat tcaaaatccg ccaccgcacg caggccacga atcagcaccg
    25921 tagcgtgttg attacgggcg aagttcgcca tcaaatcact aaacccgacc acttccacgt
    25981 tccccagatg cgcggttgcc tgctgtgcca gctcaacacg ctcttccagg gtaaacatcg
    26041 gttttttact ggggctggcg gcaatcgcca gaataacgtg atcgaacatt tgcgtggcgc
    26101 gcgtcacgat atcgatatga ccattggtaa tgggatcgaa agtacccgga taaatcgccc
    26161 gtttttgcat aacaacctca atgcgttttc ggtggcaggt aaggttccag cagttgaaga
    26221 agacgctgta gcgcgccctg gttttgatac agtacttcaa cggcatgacg gccataaaaa
    26281 ctacggtaat cggcgtcggt gagtaaagag gaaacctctt ttgcaagcgt agtggcatcg
    26341 gtaacggtaa tcagcccgct tgcctgttcc agccgcgcgc aaatgtcttt aaagttaaaa
    26401 gtatgcggcc ccatcaatac cggaattgcg tgtgcggcag cttccagcgg attatgccca
    26461 ccacgttcaa ccagtgaacc gccaacaaag gcgagatcgg caatgccgta cagtaacatc
    26521 agctcgccca tcgtatcgcc aaccacaacc tgcgtgctgg aggaagggac ttcccctgaa
    26581 gagcgtgtga tatagcttag tccagcctgg cggacaaggt taatcgcatc cgggaagcgt
    26641 tccggatgac ggggtaccag gatgagcagt aaattcggga attgctgtaa caatgcctga
    26701 tgtgcggcga tcaccacact ctcttcgcct tcgtgagtgc tggtggcaat ccataccggg
    26761 cggtgtggtg cccactggcg gcgcagcgtc acggctttag cagccaactg cggcgttaca
    26821 gaaatatcga atttcaggct accggtaacg gttacctggt tgttttttgc gcccagcgcc
    26881 acaaaacgtg caccatcttc ttcattttgg gcggcaatca gcgtaatacg acgcagcaag
    26941 cgacggacga atttacccag tttggcataa cctgcggccg agcgggcaga gagtcgcgcg
    27001 ttagcgatca ccagcggaat tttacgttta tgtagcgccg caatcaggtt aggccatagt
    27061 tcggtttcca taatcaacac cagtttaggg tcgactttat tcaggaaacg gttgagtgca
    27121 tcgggcagat catacggcag ataaacgtgc tgaacatcct tcccgaaagc cgattgtacg
    27181 cgctccgaac cggttggcgt catggttgtt acggtaatcg gtaaatcagg ataacgatga
    27241 cgcagcgcgc gcaccaacgg gatcgccgcc agagtttcac cgacggagac ggagtgcagc
    27301 ataatgccgc ctggttttag cggatggcgg taaaaaccgt aacgttcacc ccagcgtttt
    27361 cgataggccg gagccttacg tccgcgcacc cagagccgta tccagatcag cggctgaata
    27421 aggtagagaa gggcggtgta aagcaattcg agcatagtaa atagctgact tatggatgtg
    27481 ctggggattc tatgtattta gctgtggctt taccattact tttcccgttt ttgacttaaa
    27541 tagcaattta cccgccaatt cttgctgaac atctatactt tttttgtatt tttatgcgat
    27601 tcattgattt ctgctgtaca tgcaggaagg aatcgcagag caacagtgaa agaatatggt
    27661 tgctaatgat tatatttgat tgatttcttt attgaaaatc aacaatgtgc ttaaaaatca
    27721 ttcgggtcgg attgccgcga aagaagtgat acactagcgc gtcaatatca gtgcggtatc
    27781 agtattcagg tagctgttga gcctggggcg gtagcgtgct ttttctgctt caccttaatc
    27841 ggataatctc aacaaaaaga gttacttgtg gataagccat ttcgaagaat tttgctcatt
    27901 aagatgcgtt ttcatgggga tatgttatta actactcccg tcattagttc gctgaaaaaa
    27961 aattaccctg acgcaaaaat cgatgtgctg ctttatcagg acaccatccc gatcctgtct
    28021 gaaaatccag agattaacgc gctctacggc ataaaaaata aaaaagcaaa agcctcagaa
    28081 aaaattgcca acttttttca tctcatcaag gtattacgtg ccaataagta tgaccttatc
    28141 gtcaatctta ccgatcaatg gatggttgct atactggttc gcttattaaa tgcccgtgtg
    28201 aaaatttccc aggattatca tcatcggcag tctgcttttt ggcgtaaaag tttcacccat
    28261 ttggtgccgt tgcagggtgg aaatgtggtg gaaagtaact tatccgtgct gacaccattg
    28321 ggacttgatt cgttggtgaa gcagacaacc atgagttacc cgcctgcaag ctggaaacgt
    28381 atgcgtcgcg aacttgatca cgctggtgtt ggacaaaatt atgtggttat ccaacctacg
    28441 gcgcggcaaa tcttcaaatg ctgggacaac gccaagtttt ccgctgtgat tgatgcctta
    28501 catgctcgtg gttatgaagt cgttctgacg tccggcccag ataaagacga tctggcctgc
    28561 gtcaatgaaa ttgcgcaggg atgccagacg ccaccagtaa cggcgctggc tggaaaggtg
    28621 accttcccgg aacttggtgc gttaatcgat catgcgcagc tgtttattgg cgttgattcc
    28681 gcaccggcgc atattgccgc tgcagttaat acgccgctga tatcgctgtt tggtgcgaca
    28741 gaccatattt tctggcgtcc ctggtcaaat aacatgattc aattctgggc gggagattac
    28801 cgggaaatgc caacgcgcga tcagcgtgac cgaaatgaga tgtatctttc ggttattccg
    28861 gcggcagatg tcattgctgc tgtcgataaa ttactgccct cctccacgac aggtacgtcg
    28921 ttatgatcgt tgctttttgt ttatataaat attttccctt tggcggtttg cagcgcgatt
    28981 ttatgcgtat tgctcagaca gtcgccgccc gaggtcatca tgttcgggtt tatacccagt
    29041 cgtgggaggg cgaatgccct gatgtatttg aactgatcaa agtgccggtt aaatcgcata
    29101 ccaatcacgg gcgcaatgca gagtattttg cctgggtgca aaaacattta cgcgaacatc
    29161 ccgtcgataa agtcgttgga ttcaacaaaa tgccggggct ggacgtttat tatgccgctg
    29221 atgtttgtta tgccgagaaa gtagcgcagg aaaaaggctt tttctatcgc ctgacgtcac
    29281 gttatcgcca ttatgccgcc tttgagcggg caaccttcga acagggcaag ccgacacagc
    29341 tgctgatgct gacagataag caaatcgccg atttccagaa acattatcag actgaagcgg
    29401 agcgttttca tattctgcca ccggggattt atcctgatcg taaatatagc cagcagccag
    29461 ctaatagccg tgaaatcttc cgtaagaaga atggaataac cgaacaacaa tatttattgt
    29521 tgcaggtcgg ttcagacttc acgcgtaaag gtgtcgatcg ttccattgaa gcacttgctt
    29581 cgttaccgga ttcgctgcgc cacaacacat tgctatatgt tgttgggcag gataaaccgc
    29641 gaaaatttga ggcgctggca gaaaaacgcg gcgtgcgcag taatgttcac ttcttctcgg
    29701 ggcgcaacga tgtctcggaa ttaatggcgg cggcggattt attactgcat cctgcctacc
    29761 aggaagcggc gggaattgtg ctgctggaag cgattactgc aggattaccg gtactaacaa
    29821 cagccgtttg tggctatgcg cattatattg tcgaggccaa ttgcggcgag gctattgttg
    29881 agccattccg ccaggaaaca ttgaatgaga ttttacgcaa agcgttaacg caatcttcat
    29941 tgcgccaggc ctgggcggaa aatgcgcgac attatgctga tacacaagat ttatacagtc
    30001 tgccagagaa agcggcggat atcataacgg gtggtctgga tggttgaact taaagagccg
    30061 tttgccacgt tatggcgcgg taaagatcct tttgaggaag ttaaaacctt gcagggtgag
    30121 gtatttcgtg aactggaaac tcgccgtact ctgcgctttg aaatggcggg caaaagctat
    30181 tttctcaaat ggcatcgcgg cacgaccctg aaagagataa tcaaaaattt actctcattg
    30241 cggatgccag tattaggcgc agaccgcgaa tggaatgcga ttcatcgact gcgggatgtc
    30301 ggcgttgata ctatgtatgg ggtggcattt ggcgaaaaag gcatgaatcc gctgaccaga
    30361 acttcattta ttattaccga agatctgaca ccaaccataa gtctggaaga ttactgtgct
    30421 gactgggcga ctaaccctcc agatgttcgc gtaaagagta tgcttattaa gcgtgtcgcg
    30481 acgatggtgc gcgatatgca tgctgcgggc attaaccacc gtgactgtta tatctgtcat
    30541 ttcctgctgc acttgccttt ttccggtaag gaagaggagt taaaaatttc ggtaattgac
    30601 ctgcaccggg cgcagcttcg cacgcgcgtt ccacgtcgtt ggcgcgataa agatcttatt
    30661 gggctttatt tttcttcgat gaatatcggc ctgactcagc gggatatctg gcggtttatg
    30721 aaagtgtatt ttgccgcccc gcttaaagac attctcaagc aggaacaagg actgctgtcg
    30781 caagcagaag caaaagccac aaaaatcagg gaaagaacga ttcgaaaatc gttgtaattt
    30841 ccggagagaa atatgagtgc ccactatttt aatccacaag agatgatcaa taagacaatc
    30901 atcttcgatg aaaggccagc ggcgtcagtg gcatcatcat tccatgttgc ttatggcatt
    30961 gataaaaact ttctttttgg ttgtggtgtt tcaatcacgt cagttttgtt acataacaac
    31021 gacgtgaatt ttgttttcca cgtttttatt gatgatatcc ctgaagccga tatccagcgt
    31081 ttagcccaat tggcgaaaag ctatcgtacc tgtatccaga tccatctggt aaattgtgaa
    31141 cggcttaagg cattaccgac gaccaaaaat tggtctattg ccatgtattt ccgttttgta
    31201 attgcagatt actttattga tcaacaagat aagatccttt acctggatgc tgatatcgcc
    31261 tgtcagggaa acttaaagcc gctgataaca atggatcttg ccaataacgt tgctgctgtt
    31321 gttactgaac gcgatgctaa ctggtggtcg ttacgtgctc aaagtctgca gtgtaatgaa
    31381 cttgaaaagg gttactttaa ttcaggtgtc ctgttaatta atacactagc gtgggcgcag
    31441 gagtccgttt ctgctaaagc gatgtcgatg cttgctgata aagccatcgt ttcccgttta
    31501 acctatatgg atcaagatat ccttaatctt atcctgttag ggaaagttaa attcattgat
    31561 gctaaat
//
LOCUS       DAPGOF010000045        31081 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.045, whole genome shotgun sequence.
ACCESSION   DAPGOF010000045 DAPGOF010000000
VERSION     DAPGOF010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31081
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.045"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            169..984
                     /locus_tag="RHV13_001745"
     CDS             169..984
                     /locus_tag="RHV13_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1241285.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEHHMPLLRIFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            1397..1642
                     /locus_tag="RHV13_001746"
     CDS             1397..1642
                     /locus_tag="RHV13_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1241286.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMVGIRQIRLIT"
     gene            complement(1659..2330)
                     /locus_tag="RHV13_001747"
     CDS             complement(1659..2330)
                     /locus_tag="RHV13_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HDV1241287.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            2477..2752
                     /locus_tag="RHV13_001748"
     CDS             2477..2752
                     /locus_tag="RHV13_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDV1241288.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            complement(2853..4439)
                     /gene="prpR"
                     /locus_tag="RHV13_001749"
     CDS             complement(2853..4439)
                     /gene="prpR"
                     /locus_tag="RHV13_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HDV1241289.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPPMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKIPAPRLLTPQQALEKFKGDK
                     TAAANYLGISRTTFWRRLKN"
     gene            4678..5568
                     /gene="prpB"
                     /locus_tag="RHV13_001750"
     CDS             4678..5568
                     /gene="prpB"
                     /locus_tag="RHV13_001750"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HDV1241290.1"
                     /translation="MSLHSPGKAFRAALSKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARSQVK"
     gene            5913..7082
                     /gene="prpC"
                     /locus_tag="RHV13_001751"
     CDS             5913..7082
                     /gene="prpC"
                     /locus_tag="RHV13_001751"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HDV1241291.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            7116..8567
                     /gene="prpD"
                     /locus_tag="RHV13_001752"
     CDS             7116..8567
                     /gene="prpD"
                     /locus_tag="RHV13_001752"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HDV1241292.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTGMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRARLEQMPVNEYLDLYVI"
     gene            8607..10493
                     /gene="prpE"
                     /locus_tag="RHV13_001753"
     CDS             8607..10493
                     /gene="prpE"
                     /locus_tag="RHV13_001753"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HDV1241293.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            11105..12364
                     /gene="codB"
                     /locus_tag="RHV13_001754"
     CDS             11105..12364
                     /gene="codB"
                     /locus_tag="RHV13_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HDV1241294.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
     gene            12354..13637
                     /gene="codA"
                     /locus_tag="RHV13_001755"
     CDS             12354..13637
                     /gene="codA"
                     /locus_tag="RHV13_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HDV1241295.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(13770..14669)
                     /gene="cynR"
                     /locus_tag="RHV13_001756"
     CDS             complement(13770..14669)
                     /gene="cynR"
                     /locus_tag="RHV13_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414872.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HDV1241296.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDMLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAQHHPLAVHEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISPAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALEECADVGGNESR"
     gene            14779..15438
                     /gene="cynT"
                     /locus_tag="RHV13_001757"
     CDS             14779..15438
                     /gene="cynT"
                     /locus_tag="RHV13_001757"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HDV1241297.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            15469..15939
                     /gene="cynS"
                     /locus_tag="RHV13_001758"
     CDS             15469..15939
                     /gene="cynS"
                     /locus_tag="RHV13_001758"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HDV1241298.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARQVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            15945..17126
                     /gene="cynX"
                     /locus_tag="RHV13_001759"
     CDS             15945..17126
                     /gene="cynX"
                     /locus_tag="RHV13_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HDV1241299.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSALLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAALTPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSAREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLLLA
                     LALQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLLALDHSVQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFSQLWVKE
                     A"
     gene            complement(17229..17840)
                     /gene="lacA"
                     /locus_tag="RHV13_001760"
     CDS             complement(17229..17840)
                     /gene="lacA"
                     /locus_tag="RHV13_001760"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HDV1241300.1"
                     /translation="MNMPMTERIKAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
                     EKRESLIKEMFATVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSVVIKDIPPNVVAAGVPCRVIREINDRDKQYYFRDYKVESSV"
     gene            complement(17906..19159)
                     /gene="lacY"
                     /locus_tag="RHV13_001761"
     CDS             complement(17906..19159)
                     /gene="lacY"
                     /locus_tag="RHV13_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HDV1241301.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            complement(19211..22285)
                     /gene="lacZ"
                     /locus_tag="RHV13_001762"
     CDS             complement(19211..22285)
                     /gene="lacZ"
                     /locus_tag="RHV13_001762"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HDV1241302.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATHFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGEADRVTLGLNVENPKLWSAEIPNIYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTSATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGRTIEVTSEYLFRHSDNELLHWTVALDGKPLASGEVPMNVAPQGKQVIE
                     LPELPQPESTGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSAPHAIPQ
                     LTTSETDFCIELDNKRWQFNRQSGFLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLDDAVLITTVHAWQHQGKTLFISRKT
                     YRIDGSSQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGSHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLLWCQK"
     gene            complement(22408..23490)
                     /gene="lacI"
                     /locus_tag="RHV13_001763"
     CDS             complement(22408..23490)
                     /gene="lacI"
                     /locus_tag="RHV13_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HDV1241303.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISIVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            complement(23567..24514)
                     /gene="mhpR"
                     /locus_tag="RHV13_001764"
     CDS             complement(23567..24514)
                     /gene="mhpR"
                     /locus_tag="RHV13_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator MhpR"
                     /protein_id="HDV1241304.1"
                     /translation="MIFYCALSIGRVFSATIKTCPNVHQVHHVVLTIEMSINMQNNEQ
                     TEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLHRTTVRRLLETLQEEGYVRRS
                     PSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLREVVWPTDVSTLDVDAMVVRE
                     TTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQERKELIEMLASRPGDDYQLAR
                     EPLKLQAILARARKEGYGQNYRGWDQEEKIASIAVPLRSEQRVIGCLNLVYMASAMTI
                     EQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHLR"
     gene            24591..26255
                     /locus_tag="RHV13_001765"
     CDS             24591..26255
                     /locus_tag="RHV13_001765"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HDV1241305.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDDVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGVSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LNTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLFDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            26257..27201
                     /gene="mhpB"
                     /locus_tag="RHV13_001766"
     CDS             26257..27201
                     /gene="mhpB"
                     /locus_tag="RHV13_001766"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxyethylcatechol 2,3-dioxygenase"
                     /protein_id="HDV1241306.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            27219..28085
                     /gene="mhpC"
                     /locus_tag="RHV13_001767"
     CDS             27219..28085
                     /gene="mhpC"
                     /locus_tag="RHV13_001767"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HDV1241307.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            28095..28904
                     /gene="mhpD"
                     /locus_tag="RHV13_001768"
     CDS             28095..28904
                     /gene="mhpD"
                     /locus_tag="RHV13_001768"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HDV1241308.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQFDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMVSL
                     GEPLCAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            28901..29851
                     /gene="mhpF"
                     /locus_tag="RHV13_001769"
     CDS             28901..29851
                     /gene="mhpF"
                     /locus_tag="RHV13_001769"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HDV1241309.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            29848..30861
                     /gene="dmpG"
                     /locus_tag="RHV13_001770"
     CDS             29848..30861
                     /gene="dmpG"
                     /locus_tag="RHV13_001770"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HDV1241310.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAVDKLGWQHGADLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     DNK"
BASE COUNT         7132 a         8159 c         8551 g         7239 t
ORIGIN      
        1 aataatatca aagtagcagt aaaacctata acgtaaattc aaattgttaa attaactccc
       61 tccagtacac aatacttcac acgttagtta tgagcgattt ctgatagtgc ctggtttaat
      121 cagagcttta ttatctgcga cgtttatttt tatttaagag agatcgcgat gatatcatta
      181 aaagctccgc acaataattt gatgccatat acgcaacaaa gcatacttaa tacggttaaa
      241 aacaatcagt taccagagga tatcaaaagc tccctggttt cctgtgtcga tatattcaag
      301 gttttgatta aacaatatta tgattatcct tatgattgtc gtgatgattt agtcgatgac
      361 gacaaactca ttcatctcat ggctgccgta cgagactgtg agtggtcaga cgataacgca
      421 ctcaccataa atgtgcagtt taatgatttc cctggatttt atgactggat ggattaccct
      481 gatcacccgg ttaagtttgt ttttcgcata cttgaaaatc aaaaagggac agtttgggta
      541 tatgaccagg atgatgcgtt tcttgatatt aaagcaaacg tgcaggctgg acgctttacc
      601 gggcttaaga aattagtgca gtttatcgat tcagtgcgaa cagattgcaa atgtattctt
      661 cttgaacacc atatgccttt gctgagaatt ttccctaaag ggaaagagtg tatgcatgtg
      721 gaaaaatggc ttcgcgaaat gtcgtctatt ccggagacag acgcgcccat taagcaggca
      781 cttgcgcatg gattattatt acacttaaaa aatatctacc ctgtttttcc tgaatcactg
      841 gtgatgttat tgctctctgt tctggacgta aaaacataca gagatgatgc gcgattaaat
      901 gaatggatat ctaatagagt ccaggaatta ggtgacagat attatcctgt aaataagcac
      961 gttaaaatcc gctacactct ttagttcaga aagggttcca cctgcaagtt gttattccac
     1021 cagagagtga atcgcaatgc aggtaatcat ttcatctgta tatccgtatg ccaaatggca
     1081 ttccaccgcc tttttcgccg atattttgtt gtgatcggcg acgcttcgct taaaaaagca
     1141 ccagtagtgg tttcgcagcc atgcggtgta taaaaaatga tctcatgcag atgttttgtg
     1201 aatgtgtggg ttgacattca tatgaaaaaa atcataattc catcatgttt gtgtgggatc
     1261 ttttctgtac cttacgcatc gcactcaagc ttgacagaaa atatgctgta aggctcatat
     1321 caaaaaccgc cattagctca tcaggaagag cagacgacaa ccaggattgt tgtatggtac
     1381 ggggttcgag gcctcgatgg cggtccaact tttcaaaact cttctgaatc agatcccctt
     1441 attatcatcc ctgcaaagtg gaacattgcc attgtttggc tattcaggac gggtcagacc
     1501 aatgaaaaaa gcccacacag gggagagtgg gctgaaatgg gaagctaaag actcaagtaa
     1561 acttatcgga aataaggacc acgcattacg ggggctatca tcgccgatgg tcgggattcg
     1621 gcaaatcagg ttgattacgt agagagtaaa ttatctgctc accgctgcgt cacaccttcg
     1681 taaatcaggc gtagcgcgaa tacaccaata attgcaccaa taacccgact ggcaacgcgt
     1741 tgcatacgcc cataagcacg acgcacagcg ggcaaagaaa acgcctgact aagaaaaact
     1801 cgccagataa ttgatgcgag cacaatcccc gcccaggcca ttaaacgtgc ccatgttggt
     1861 gtttcggcat ttaatgttac tgagaaaata ctaataaaaa ataaaacggt ttgtgggtta
     1921 gagagatcgg taattaatcc acggcgaaaa aagacatacc agggggcgct aatcggttgt
     1981 tgtagtgtgc tcatttgcgg tgttgactgg cggcgcatgc tgcaccacgc aaaccataag
     2041 agataagcgc cgccgacgat tctgataagc gaaaaaatct cctcacactg cgtaattagc
     2101 gttgcaagac caaacaaacc caacccggaa taaaatgcat cgcccagcgc cacgcccagc
     2161 ccggtcagca cccctgcgcg tcgaccggaa gccaggctgg tttgtactac cacaaagaga
     2221 ttggctcccg gattaaaaaa agtaatcacg aacagtccta cggtcaggta aacggcgtgc
     2281 aaaggatcca tagtgatttc atccataaat aagtgaagta acttcatcat atttctacca
     2341 aaaataatcg ggtgcgagag agatcacaaa gtgtcttatt tccggttact ggcgtttatg
     2401 ccttgactga actaattatt aatcaaccca ataatgtggg tgggtgatag tgtgataaca
     2461 actctggagc cgtaatatga aaataatctc taaaatgtta gtcggtgcgt tagcgtttgc
     2521 cgttaccaat gtctatgccg ctgaattgat gactaaagcg gaatttgaaa aagtcgaatc
     2581 gcaatatgaa aaaataggtg atatttcaac cagtaatgaa atgtcgaccg cagatgcgaa
     2641 agaggatttg atcaaaaaag cggatgaaaa aggggctgat gtgttggtac tgacctccgg
     2701 tcaaactgac aataagatcc acggcacggc aaatatttat aagaagaagt aattctgaac
     2761 cctatgtaaa catccccgat gcgtaagttt atcggtgatc atcatctatt gaaatttatg
     2821 ccggataaag cgttcgcgct gcattcggca gttcagtttt tcagccgccg ccagaacgtc
     2881 gtccggctga tgcctaaata attcgccgct gctgttttat cgcctttaaa tttctccagt
     2941 gcctgttgtg gtgtcagtaa gcgtggagcg ggaattttcg ccgactcgcg cgccagctcc
     3001 ggcagtagca gctgcaaaaa ctgcggcgtt aaatccggcg tcggttccac acttaaaaac
     3061 agcgccagtc gctccatcat attgcgcagt tctcgaatat tgcccggcca gtcgtagtgc
     3121 accagcacgg tttcgcttgc ctgtaatccc tggcgtaatg cggcagaaaa cggggcggag
     3181 agcgccgcca gagacacttt caaaaagctt tccgccagcg gaagaatatc cgccacccgc
     3241 tcgcgcagtg gtggcaattg cagacgcaaa atactcagcc gataaaacag gtcacggcga
     3301 aactgccctt gccgcatatc ttcttccaga ttgcagtgag tggcgctaat gacccgtaca
     3361 tccaccggaa caggctgatg cccgccgacg cgggtgacct ctttttcttc cagcacccgc
     3421 agcagccggg tctgcaaagg tagcggcatt tcgccaatct catccaaaaa cagcgtgcca
     3481 ccgtgggcga tttcaaacag cccggcacga cctccgcgtc gcgagccggt aaacgcccct
     3541 tcctcatagc caaacagctc cgcttccagc agcgattcgg caatcgcccc gcagttgacg
     3601 gcaacaaacg gatgcgactt tttgccctgt cgcgcatcgt ggcgggcaaa atattcccga
     3661 tgaattgcct gggccgccag ctctttgccc gtccccgttt ccccctcaat caacaccgct
     3721 gcactggagc gggcatacag caaaatagtc tgccgtactt gttccatcgg tggtgattga
     3781 ccgagcatat cgcccagcac gtaacgagtt ctcagggcgt tgcgggtggc atcgtgagtg
     3841 ttatggcgta acgacatgcg cgtcatatcc agcgcatcgc tgaacgcctg gcgcacggtg
     3901 gcggcggaat agataaaaat tccggtcatt ccggcttctt ctgccagatc ggtaatcagc
     3961 cccgcaccga ccaccgcttc ggtgccgtta gcttttagct cgttaatctg cccgcgtgcg
     4021 tcttcctcgg taatgtagct acgttgatcg aggcgcaaat taaaggtttt ttgaaacgcc
     4081 accagtgccg gaatagtttc ctgataagtg acaacgccga tagaagaggt gagttttccg
     4141 gcttttgcca gtgcctgtaa cacatcgtag ccgctcggtt taatcaaaat aactggcact
     4201 gataggcggc ttttcaggta cgcgccgtta gagccagcag cgatgatggc gtcacagcgt
     4261 tcgtttgcca gtttcttgcg gatgtaggtc actgcttttt caaagccaag ctgaataggg
     4321 gtaatgttcg ccaggtgatc aaactcgagg ctgatatcgc gaaacagctc gaacaggcgc
     4381 gttacagata ccgtccagat aaccggtttg tcgtcattca gccgtggtgg atgtgccata
     4441 gcgcaccgca aagttaagaa accgaatatt gggtttagcc ttgtttcata attgttgcaa
     4501 tgaaacgcgg tgaaacattg cctgaaacgt taactgaaac gcatatttgc ggattagttc
     4561 atgactttat ctctaacaaa ttgaaattaa agatttaatt ttattaaggc aattgtggca
     4621 caccccttgc tttgtgttta tcaacgcaaa taacaagttg ataacaaagg atgggctatg
     4681 tctctacact cgccaggtaa agcgtttcgc gctgcactgt ctaaagaaaa tccattgcag
     4741 attgttggca ccattaacgc caaccatgcg ctgttggcgc agcgtgccgg atatcaggca
     4801 atttatctct ccggcggtgg cgtggcggca gggtcgctgg ggctgcccga tctcggtatt
     4861 tctacccttg atgatgtgct gaccgacatt cgccgtatca ccgacgtttg ttcgctgccg
     4921 ctgctggtgg atgcggatat cggttttggt tcttcggcct ttaacgtggc gcgcaccgtg
     4981 aaatcgatga ttaaagctgg tgcggcagga ttgcatattg aagatcaggt tggtgcgaaa
     5041 cgctgcggtc atcgtccgaa taaagcgatc gtctcgaaag aggagatggt ggatcggatc
     5101 cgcgcggcgg tggatgcgaa aaccgatcct gattttgtga tcatggcgcg caccgatgcg
     5161 ctggcggtag aggggctgga tgcggcgatc gagcgtgcgc aggcctatgt tgaagcgggt
     5221 gccgagatgt tgttcccgga ggcgattacc gaactcgcca tgtaccgcca gtttgccgat
     5281 gcggtgcagg tgccaatcct cgccaacatt accgaatttg gcgccacgcc gctgtttacc
     5341 accgacgaat tacgcagcgc tcatgtcgcg atggcgctgt acccgctttc agcgtttcgc
     5401 gccatgaacc gcgccgccga acatgtctac aacgtcctgc gccaggaagg cacgcagaaa
     5461 agtgtcatcg acaccatgca aacccgcaac gagctgtacg aaagcatcaa ctactaccag
     5521 tacgaagaga agctcgacga cctgtttgcc cgtagccagg tgaaataaaa aacgcccgtt
     5581 ggttgtattc gacaaccgat gcctgatgcg ccgctgacgc gacttatcag gcctacgggt
     5641 gtactgaact gtaggtcgga taagacgcat ggcatcgcat ccgacaaccg atgcctgatg
     5701 cgtcgctgac gcggcttatc aggcctacgg ggtgaactga actgtaggtc ggataagacg
     5761 catggcgtcg catccgacaa ccgatgcctg atgcgccgct gacgcgactt atcaggccta
     5821 cgggtgtact gaactgtagg tcggataaga cgcatggcgt cgcatccgac aacaatctcg
     5881 accctacaaa tgataacaat gacgaggaca atatgagcga cacaacgatc ctgcaaaaca
     5941 gtacccatgt cattaaaccg aaaaaatctg tggcgctttc tggcgttccg gcgggcaata
     6001 cggcgctctg caccgtgggt aaaagtggca atgacttgca ttaccgtggc tacgatattc
     6061 ttgatctggc ggaacattgc gaatttgaag aagtggcgca tctgctgatc cacggcaaac
     6121 tgccgacccg tgacgaactc gccgcctaca aaacgaaact gaaagccctg cgcggtttac
     6181 cggctaacgt gcgtaccgtg ctggaagcct taccggcggc gtcacacccg atggatgtta
     6241 tgcgcaccgg cgtttccgcg ctcggctgca cgctgccaga aaaagagggg cataccgtct
     6301 ctggcgcgcg ggatattgcc gacaaactgc tggcgtcact tagctcgatt cttctctact
     6361 ggtatcacta cagccataac ggcgaacgca tccagccgga aaccgatgac gactccatcg
     6421 gcggtcactt cctgcatctg ctgcatggcg aaaagccatc gcaaagctgg gaaaaggcga
     6481 tgcatatttc gctggtgctg tacgccgaac acgagtttaa cgcctccacc tttaccagtc
     6541 gggtgattgc gggtactggc tctgatatgt attccgcgat tattggcgcg attggtgcac
     6601 tgcgcgggcc gaagcacggc ggggcgaatg aagtgtcgct ggagatccag caacgctacg
     6661 aaacgccgga cgaagccgaa gccgatatcc gcaagcgggt ggaaaacaaa gaagtggtca
     6721 ttggttttgg gcatccggtt tataccatcg ccgacccgcg ccaccaggtg attaaacgtg
     6781 tggcgaagca gctctcgcag gaaggcggct cgctgaagat gtacaacatc gccgatcgcc
     6841 tggaaacggt gatgtgggag agcaaaaaga tgttccccaa tctcgactgg ttctccgctg
     6901 tttcctacaa catgatgggc gttcccaccg agatgttcac accactgttt gttatcgccc
     6961 gcgtcaccgg ctgggcggcg cacattatcg aacaacgtca ggacaacaaa attatccgtc
     7021 cttccgccaa ttatgttgga ccggaagacc gccagtttgt cgcgctggat aagcgccagt
     7081 aaacctctac gaataacaat aaggaaacgt acccaatgtc agctcaaatc aacaacatcc
     7141 gcccggaatt tgatcgtgaa atcgttgata tcgtcgatta cgtgatgaac tacgaaatca
     7201 gctccaaagt ggcctacgac accgcacatt actgcctgct cgacacgctc ggctgcggtc
     7261 ttgaagctct cgaataccca gcctgtaaaa aactgctggg gccaattgtc cccggcaccg
     7321 tcgtacctaa cggcgtgcgc gtccccggaa ctcagttcca gctcgacccc gtccaggcgg
     7381 catttaacat cggcgcgatg atccgttggc tcgatttcaa cgatacctgg ctggcggctg
     7441 agtgggggca tccttccgac aacctcggcg gcattctggc aacggcagac tggctttcgc
     7501 gcaacgcggt cgccagcggc aaagcgccgt tgaccatgaa acaggtgctg accggaatga
     7561 ttaaagccca tgaaattcag ggctgcatcg cgctggaaaa ctcctttaac cgcgtcggcc
     7621 tcgaccacgt tctgttagtg aaagtggctt ccaccgccgt ggtcgccgaa atgcttggcc
     7681 tgacccgcga ggaaattctc aacgctgttt cgctggcgtg ggtggacggt cagtcgctgc
     7741 gcacctatcg ccatgcgccg aacaccggca cgcgtaaatc ctgggcggcg ggcgatgcca
     7801 cttcccgcgc ggtacgtctg gcactgatgg cgaaaacggg cgaaatgggt tacccgtcag
     7861 ccctgactgc gccggtatgg ggcttctacg acgtctcctt taaaggtgaa tcgttccgct
     7921 tccagcgtcc gtacggttct tacgtcatgg agaatgtgct gttcaaaatc tccttcccgg
     7981 cggagttcca ctcccagacg gcagttgaag cggcgatgac gctctatgaa cagatgcagg
     8041 cagcaggcaa aacggcggcg gatatcgaaa aagtgaccat tcgcacccac gaagcctgta
     8101 ttcgcatcat cgacaaaaaa gggccgctca ataacccggc agaccgcgat cactgcatcc
     8161 agtacatggt ggcgatcccg ctgctattcg ggcgcttaac ggcggcagat tacgaggaca
     8221 acgttgcgca agataaacgc atcgacgccc tgcgcgagaa gatcaattgc tttgaagatc
     8281 cggcgtttac cgctgactac cacgacccgg aaaaacgcgc catcgccaat gccattactc
     8341 ttgagttcac cgacggcacg cgctttgaag aagtggtggt ggagtacccc attggtcatg
     8401 ctcgccgccg tcaggatggc attccgaagc tggtggataa attcaaaatc aatctcgcgc
     8461 gccagttccc gactcgccag cagcagcgca ttctggaggt ttctctcgac agagctcgcc
     8521 tggaacagat gccggtcaat gagtatctcg acctgtacgt catttaagta aacggcggta
     8581 aggcgtaagt tcaacaggag agcattatgt cttttagcga attttatcag cgttctatta
     8641 acgaaccgga gcagttctgg gccgagcagg cccggcgtat tgactggcag acgcccttta
     8701 cgcaaacgct cgatcacagc aatccgccgt ttgcccgttg gttttgtgaa ggccgaacca
     8761 atttgtgcca taacgctatc gatcgctggc tggagaaaca gccagaggcg ctggcgctga
     8821 ttgccgtctc ttcggaaaca gaagaagagc gcacctttac ctttcgccag ctgcatgacg
     8881 aagtaaacgc ggtggcgtca atgctgcgct cactgggcgt gcagcgtggc gatcgggtgc
     8941 tggtgtatat gccgatgatt gccgaagcgc atattaccct gctggcttgc gcgcgcattg
     9001 gcgctattca ctcggtggtg tttgggggat ttgcctcgca cagcgtggcg gcgcgaattg
     9061 atgatgctaa accggtgctg attgtctcgg ctgatgccgg agcgcgcggt ggcaaaatca
     9121 ttccctataa aaaattgctc gacgatgcga taagtcaggc gcagcatcag ccgcgccacg
     9181 ttttgctggt ggatcgcggg ctggcgaaaa tggcgcgcgt cagcgggcgg gatgtcgatt
     9241 tcgcgtcgtt gcgccatcaa cacatcggcg cgcgggtgcc ggtggcgtgg ctggaatcca
     9301 acgaaacctc ctgcattctc tacacctccg gcacgaccgg caaacctaaa ggcgtgcagc
     9361 gtgacgtcgg cggatatgcg gtggcgctgg cgacctcgat ggacaccatt tttggcggca
     9421 aagcgggcgg tgtgttcttt tgtgcttcgg atatcggctg ggtggtgggg cattcgtata
     9481 tcgtctacgc gccgctgctg gcggggatgg cgactatcgt ttacgaagga ttaccgacct
     9541 ggccggactg cggcgtgtgg tggaaaatcg tcgagaaata tcaggttagc cggatgtttt
     9601 cagcgccgac cgccattcgc gtgctgaaaa aattccctac cgctgaaatt cgcaaacacg
     9661 atctctcgtc gctggaagtg ctctatctgg ctggagaacc gctggacgag ccgaccgcca
     9721 gttgggtgag caatacgctg gatgtgccgg tcatcgacaa ctactggcag accgaatccg
     9781 gctggccgat tatggcgatt gctcgcggtc tggacgacag gccgacgcgt ttgggtagcc
     9841 ccggcgtgcc gatgtatggc tataacgtgc agttgctcaa tgaagtcacc ggcgagccgt
     9901 gcggcgtcaa cgagaaaggg atgctggtgg tggaagggcc attgccgccg gggtgtattc
     9961 agaccatctg gggcgacgat gaccgctttg tgaagacgta ctggtcgctg ttttcccgcc
    10021 cggtgtacgc cacctttgac tggggcatcc gcgatgctga cggttatcac tttattctcg
    10081 ggcgcactga cgatgtaatt aacgttgccg ggcatcggct ggggacgcgc gagattgaag
    10141 agagtatctc cagtcatccg ggcgttgccg aagtggcggt ggttggggtg aaagatgcgc
    10201 tgaaagggca ggtggcggta gcgtttgtca ttccgaaaga gagcgacagt ctggaagacc
    10261 gagacgtggc gcactcgcaa gagaaggcga ttatggcgct ggtggacagc cagattggca
    10321 actttggccg cccggcgcac gtctggtttg tctcgcaatt gccaaaaacg cgatccggaa
    10381 aaatgctgcg ccgcacgatc caggcgattt gcgaaggacg cgatcctggg gatctgacga
    10441 ccattgatga tcctgcgtcg ttggatcaga tccgccaggc gatggaagag taaggtattg
    10501 tcggatgcgt cgcgtggcgc atccgacacc atgcgccgat gcctgatgcg acgctggcgc
    10561 gtcttatcat gcctacaggc ctgaaccgta ggtcggataa ggcgctcgcg tcgcatccga
    10621 caccccgcgc cgatgcctga tgcgacgctg gcgcgtctta tcatgcctac aggtctgaac
    10681 cgtaggtcgg ataaggcgct cgcgccgcat ccgacaccgt gcgcagatgc ctgatgcgac
    10741 gctggcgcgt cttatcatgc ctacggacct gaaccgtggg tcggataagg cgctcgcgcc
    10801 gcatccgaca ccgtgtgccg atgcctgatg cgacgctgac gcgtcttatc atgcctacag
    10861 gcctgaaccg taggtcggat aaggcgctcg cgccgcatcc gacaccatgc gccgatgcct
    10921 gatgcgacgc ttaccgcgtc ttatcatgtc tgcccactgt ttttacaccg ataattttcc
    10981 ctcacctttt tgcactcatt catataaaaa atatatttcc ccacgaaaac gattgctttt
    11041 tatcttcaga tgaatagaat gcggcggatt ttttgggttt caaacagcaa aaagggggaa
    11101 tttcgtgtcg caagataaca actttagcca ggggccagtc ccgcagtcgg cgcggaaagg
    11161 ggtattggca ttgacgttcg tcatgctggg attaaccttc ttttccgcca gtatgtggac
    11221 cggcggcact ctcggaaccg gtcttagcta tcatgatttc ttcctcgcag ttctcatcgg
    11281 caatcttctc ctcggtattt acacttcatt tctcggttac attggcgcaa aaaccggcct
    11341 gaccacccat cttcttgctc gcttctcgtt tggtgttaaa ggctcatggc tgccttcact
    11401 gctgctgggc ggcacccagg tcggttggtt tggcgtcggt gtggcgatgt ttgccattcc
    11461 ggtgggcaag gcaaccgggc tggatattaa tttgctgatt gccgtctccg gtttactgat
    11521 gaccgtcacc gtcttctttg gcatttcggc gctgacggtt ctttcggtga ttgcggttcc
    11581 ggcgatcgcc tgtcttggcg gttattccgt gtggctggcc gttaacggca tgggcggcct
    11641 ggacgcctta aaagcggtcg ttcccgcaca accgttagat ttcaatgtcg cgctggcgct
    11701 ggttgtgggg tcatttatca gcgcgggtac gctcaccgct gactttgtcc gctttggtcg
    11761 caatgccaaa ctggcggtgc tggtggcgat ggtggccttt ttcctcggca actcgttgat
    11821 gtttattttc ggtgcagcgg gcgctgcggc actggggatg gcagatatct ctgatgtgat
    11881 gattgctcag ggcctgctgc tgcctgcgat tgtggtgctc gggctgaata tctggaccac
    11941 caacgataac gcgctctatg cgtcgggttt aggtttcgcc aacatcaccg ggatgtcgag
    12001 caaaaccctt tcggtgatta acggtattat cggtacggtc tgcgccttat ggctgtataa
    12061 caattttgtc ggctggctga ccttcctttc ggcagctatt cctccggtag ggggcgtgat
    12121 cattgccgac tatctgatga accgtcgccg ctatgagcac tttgcgacca cgcgtatgat
    12181 gagtgtcaat tgggtggcga ttctggcggt cgccttgggg attgctgcag gccactggtt
    12241 accgggaatt gttccggtca acgcggtatt aggtggcgcg ctgagctatc tgatccttaa
    12301 cccgattttg aatcgtaaaa cgacagcagc aatgacgcat gtggaggcta acagtgtcga
    12361 ataacgcttt acaaacaatt attaatgccc ggttaccagg caaagagggg ctgtggcaga
    12421 ttcatctgca ggacggaaaa atcagcgcca ttgatgcgca atccggcgtg atgcccataa
    12481 ctgaaaacag cctggatgcc gaacaaggtt tagttatacc gccgtttgtg gagccacata
    12541 ttcacctgga caccacgcaa accgccggac aaccgaactg gaatcagtcc ggcacgctgt
    12601 ttgaaggcat tgaacgctgg gccgagcgca aagcgttatt aacccatgac gacgtgaaac
    12661 aacgcgcatg gcaaacgctg aaatggcaga ttgccaacgg cattcagcat gtgcgtaccc
    12721 atgtcgatgt ctccgacgca acgctgactg cgttgaaagc gatgctggaa gtgaaacagg
    12781 aagttgcgcc gtggattgat ctgcaaatcg tcgccttccc gcaggaaggg attttgtcgt
    12841 atcccaacgg tgaagcgttg ctggaagagg cgttacgctt aggggcagat gtagtggggg
    12901 cgattccgca ttttgaattt acccgcgaat acggcgtgga gtcgctgcat aaaaccttcg
    12961 ccctggcgca aaaatacgac cgtctcatcg acgttcactg tgatgagatc gatgacgagc
    13021 agtcgcgctt tgtcgaaacc gttgctgccc tggcgcaccg tgaaggcatg ggcgcgcgag
    13081 tcaccgccag ccacaccacg gcaatgcact cctataacgg ggcgtatacc tcacgcctgt
    13141 tccgcttgct gaaaatgtcc ggtattaact ttgtcgccaa cccgttggtc aatattcatc
    13201 tgcaagggcg tttcgatacg tatccaaaac gtcgcggcat cacgcgcgtt aaagagatgc
    13261 tggagtccgg cattaacgtc tgttttggtc acgatgatgt cttcgatccg tggtatccgc
    13321 tgggaacggc gaatatgctg caagtgctgc atatggggct gcatgtttgc cagctgatgg
    13381 gctacgggca aattaacgat ggcctgaatt taatcaccca ccacagcgcc aggacgttga
    13441 atttgcagga ttacggcatt gccgccggaa acagcgccaa cctgattatc ctgccggctg
    13501 aaaatggatt tgatgcgctg cgccgtcagg ttccggtacg ttattctgta cgtggcggta
    13561 aggtgatcgc cagcacacaa ccggcacaaa ccaccgtata tctggagcag ccagaagcca
    13621 tcgattacaa acggtgatcg gctgggctgt gtgttgacaa agcgagcgta gtttatgccg
    13681 gatgcggcgt gaacgcctta tccggcctac gtagagcact gaactcgtag gcctgataag
    13741 cgtagcgcat caggcaattg tgtcagtggc taccgtgatt cattcccgcc aacatccgcg
    13801 cattcctcca acgccatgtg caaaaatgcc ttcgcggcgg ctgtctgcca gctatttttc
    13861 cgccgcaaca aaaccgccgt tctctccagt agtggcgggg caggagaaat agctttaagc
    13921 ccgtcatgtt gtgtggcaat cgctgctggt aacaatgtgg aaagggaagt gcggcgaatc
    13981 agctccagaa ccgcgctaat tgagttcgcc tcaatgacca cctgtggatg cagccccgct
    14041 ttctcgcagt agtggtcaat ttgctctctg gtggcaaatt ccgcgctgag cagtaccagt
    14101 ttttcatcat gcaagcgact caacgccacc tgttcatgga ctgccagcgg atgatgttgc
    14161 gccacgacta acgctaaact ttctgtcagt aaaggaattg cctccagctc cggcgaatgc
    14221 acaggcgcga aggcaatccc aacgtccaac tcgtcgcggc aaagcatatc ctcgattttc
    14281 tcctgcgaca tttcctgtag ctggagcgtg atgccgggat agcgcgcata gaaatccgcc
    14341 attaaggggc cgataaagta gctcgtaaag gtgggggtga cggcgatacg cagcgatcct
    14401 cgcgtcagat cggcaacatc atgaatcgcc cgtttacccg cccccagttc ctgtaacgcc
    14461 cggctggcgt actgtcgcca gacttctcct gcatcagtga gacgaatcgt tcgcccgcta
    14521 cggtcaaaca gcggcacgcc taaactctcc tctaactggc gaatctgctg ggaaagcgca
    14581 ggctgagaga cgtgcaacgc actggcggca cgggtgaagc tgccatgttc agccacggca
    14641 agaaaataat tgatatgtcg agagagcatt cgccaaccta taagtaaatc caatagaact
    14701 catcataaat gagactttta ccttatgaca atcggcgagt agtctgcctc tcattccaga
    14761 gacagacaga ggttaacggt gaaagagatt attgatggat tccttaaatt ccagcgcgag
    14821 gcatttccga agcgggaagc cttatttaaa cagctggcga cacagcaaag cccgcgcacc
    14881 ctttttatct cctgctccga cagccgtctg gtccctgagc tggtgacgca acgtgagcct
    14941 ggcgatctgt tcgttattcg caacgcaggt aatattgtcc cttcctacgg gccggaaccc
    15001 ggtggcgttt ctgcttcggt ggagtatgcc gtcgctgcgc ttcgggtatc tgacattgtg
    15061 atttgcggtc attccaactg tggcgcgatg accgccattg ccagctgtca gtgcatggac
    15121 catatgcctg ccgtctccca ctggctgcgt tatgccgatt cagcccgcgt cgttaatgag
    15181 gcgcgcccgc attccgattt accgtcaaaa gctgcggcga tggtacgtga aaacgtcatt
    15241 gctcagttgg ctaatttgca aactcatcca tcggtgcgcc tagcgctcga agaggggcgg
    15301 atcgccctgc acggctgggt ctacgacatt gaaagcggca gcatcgcagc ttttgacggc
    15361 gcaacccgcc agtttgtgcc actggccgct aatcctcgcg tttgtgccat accgctacgc
    15421 caaccgaccg cagcgtaacc ttatttttaa accatcagga gatgcaccat gattcagtcg
    15481 caaattaatc gcaatattcg tcttgatctt gccgatgcca ttttgctcag caaagctaaa
    15541 aaagatctct catttgccga gattgccgac ggcaccggtc tggcagaagc ctttgtgacc
    15601 gcggctttgc tgggtcagca ggcgcttcct gccgacgccg cccgccaggt cggggcgaag
    15661 ctggatctcg acgaagacgc cattctactg ttacagatga tcccactgcg tggctgcatt
    15721 gatgaccgta ttccaaccga cccaacgatg tatcgtttct atgaaatgtt gcaggtgtac
    15781 ggcacaaccc tgaaagcgtt ggttcatgag aagtttggcg atggcattat cagcgcgatt
    15841 aacttcaaac tcgacgttaa gaaagtggcg gacccggaag gtggcgaacg tgcggtcatc
    15901 accttagatg gtaaatatct gccgaccaaa ccgttctgac agccatgcgt aaccatcaaa
    15961 agacgttcac gatgctgctg gttctggtgc tgattggtct taatatgcga ccactgctca
    16021 cctccgtcgg gccactgcta ccgcaattgc gccaggcaag cggaatgagc tttagcgtgg
    16081 ctgccctgtt gaccgctctg ccggtggtta ccatgggcgg gctggcgctg gccggaagct
    16141 ggcttcatca gcatgtcagc gaacgtcgca gcgtcgccat cagtttgtta ctgattgccg
    16201 tcggtgcatt gatgcgtgag ctttaccccc aaagtgcgct gctgcttagc agcgcactgc
    16261 tcggtggggt ggggatcggc atcattcagg cggtgatgcc ttcagtgatt aaacggcggt
    16321 ttcagcagcg cacgccactg gtgatggggc tgtggtccgc ggctctgatg ggcggcggtg
    16381 gacttggtgc cgccctgacg ccctggttag ttcaacatag cgaaacctgg tatcaaacac
    16441 tcgcctggtg ggcgctgcct gccgttgttg cgctctttgc ctggtggtgg caaagcgccc
    16501 gcgaggtcgc ctcttcccac aagacaacaa ccactccggt tcgcgtggtt ttcactcctc
    16561 gcgcgtggac gctgggtgtt tacttcggtc tgattaacgg tggttacgcc agcctgattg
    16621 cctggttacc cgctttctat attgagattg gtgccagcgc gcagtacagc ggttccttgc
    16681 tggcattgat gacgcttggg caagccgcag gagctttgct gatgcctgct atggctcgcc
    16741 atcaggatcg gcgcaaactt ttgctgttgg cactggcgtt acaactggtg ggtttctgcg
    16801 gctttatctg gctgccgctg caattgccgg tattgtgggc gatggtgtgt ggtttaggtc
    16861 tgggcggcgc atttccgctc tgtttgctgc tggctctcga tcactctgtg caaccggcta
    16921 ttgctggcaa gctggtggcg tttatgcagg ggatcggttt tatcatcgcc gggcttgccc
    16981 cgtggttttc tggcgtgctg cgtagtatca gcggcaatta cctgatggac tgggcgtttc
    17041 atgcgctgtg cgtcgttggg ctgatgatta taaccctgcg ttttgcacca gcacgttttt
    17101 cgcagctgtg ggtcaaagag gcatgatgcg acgcttgttt ctgcgctttg ttcacgccgg
    17161 atgcggccta cgtagatcac tgaatttgta ggcctgataa gcgaagcgta tcaggcaatt
    17221 tttataattt aaactgacga ttcaactttg taatctctga aataatattg cttatcccgg
    17281 tcgtttattt cgcgaataac ccggcaagga acgccagcag ccacgacgtt tggtggaatg
    17341 tcttttatga cgacactacc cgcgccaata acagaattat ccccgatggt gacgcctgga
    17401 ttaatgacca catggcttcc aatccagaca ttattgccaa tcgttatcgg aaaagagtac
    17461 atctcaccgt tttttctcaa ttcatggtgt acagggtgtc ccgtaacgga aagagtaaca
    17521 ttgggtgcaa tcaatacgtt atcaccgatc gttaccgtgt agtcgtctac aatggttaaa
    17581 ttgaaatttg cataaaaatt gcggcctata tggatgttgg aaccgtaaga gaaataaacg
    17641 ggtggttcta cccaggcgtt ttcccctacc gtggcaaaca tttctttaat caggctttct
    17701 cttttttcaa cttctgatgg atgcgagtga ttaaactcat acattaacgt ttttccacga
    17761 agtctttttt ccggtaagcc ttcgcacata tcggtaaata gcttgcctgc ttttattctt
    17821 tcggtcattg gcatgttcat tgcgatcatt ccgttatgat atgttggtcg gataaggcgc
    17881 tcgcgccgca tccgacatta attgcttaag cgacttcatt cacctgacga cgcagcaggg
    17941 aaagcgggcc ggggccgcta agcgtgaaca cggaaattaa ggtgaagccc agcgccacta
    18001 gacccagcac cagataagcg ccctggaaac cgatgctttc atacatatta cccgccagta
    18061 cagacataaa aatcatcgcc agttgcttaa agaagcagaa acagaccaga taaatcgtcg
    18121 ctgaaaaacg cacttcaaac tggctggtaa tatatttaaa gcagcccacc agcaggaacg
    18181 gtacttcaaa catatgcagc gttttcagaa taaccacttc cagcgctgag gtggcgaacg
    18241 atgagccaat aatacgtaca gacataatag tgccagccag cagcagggcg tttttcccac
    18301 cgatgcgatt aatgatcagt ggcgcaaaga acataattga ggcgttaagt aattcgccca
    18361 ttgtcgttac gtagccaaat acccgcgtcc cctgttcacc ggtggcaaag aaagaagtga
    18421 agaaattagc aaactgttgg tcaaaaacat cgtaggtgca ggaaacgcca ataacataca
    18481 gtgacaaaaa ccacagtttt ggctgtctga acagttccag cgccagttta aggctaaatg
    18541 ccgaatggtt ggcacctacc gcattggcaa ccgtggcgga agagggcgca tccgttttgg
    18601 cgaaaaagag taaaacggcg aggatgagtg cacagccaga acccagccag aaaacaaact
    18661 gattattgat ggtgaacatg atgccgacaa tcgaggcaca cagcgcccag ccaacacagc
    18721 caaacatccg cgcgcgacca aattcgaaat tactgcgacg gctgactttc tcgataaatg
    18781 cctctactgc gggcgcaccg gcgttaaaac aaaagccaag ataaatacca ccaacaatcg
    18841 atcctactaa aatgttgtat tgtaacagtg gcccgaagat aaaaataaag aacggcgcaa
    18901 acatcactaa catgccggta ataatccaca gcaggtattt gcgcagcccg agtttgtcag
    18961 aaagcagacc aaacagcggt tggaataata gcgagaacag agaaatagcg gcaaaaataa
    19021 tacccgtatc acttttgctg atatggttga tgtcatgtag ccaaatcggg aaaaacggga
    19081 agtaggctcc catgataaaa aagtaaaaga aaaagaataa accgaacatc caaaagtttg
    19141 tgttttttaa atagtacata atggatttcc ttacgcgaaa tacgggcaga catggcctgc
    19201 ccggttatta ttatttttga caccagagca actggtaatg gtagcgaccg gcgctcagct
    19261 ggaattccgc cgacactgac gggctccagg agtcgtcgcc accaatcccc atatggaaac
    19321 cgtcgatatt cagccatgtg ccttcttccg cgtgcagcag atggcgatgg ctggtttcca
    19381 tcagttgttg ttggctgtag cggctgatgt tgaactggaa gtcgccgcgc cactggtgtg
    19441 agccataatt caattcgcgc gtcccgcagc gcagaccgtt ttcgctcggg aagacgtacg
    19501 gggtatacat gtctgacaat ggcagatccc agcggtcaaa acaggccgca gtaaggcggt
    19561 cgggatagtt ttcttgcggc cctaatccga gccagtttac ccgctctgct acctgcgcca
    19621 gctggcaggt caggccaatc cgcgccggat gcggtgtatc gctcgctact tcaacatcaa
    19681 cggtaatcgc catttgacta cttccatcaa tccggtaggt tttccggctg ataaataagg
    19741 tttttccctg atgctgccat gcgtggacag tggtaatcag caccgcgtcg tcaagcgtat
    19801 ctgccgtgca ctgcaacaac gccgcttcgg cctggtaatg gcccgccgcc ttccagcgtt
    19861 cgacccaggc gttagggtca atacgggtcg cttcacttac gccaatgtcg ttatccagcg
    19921 gtgcgcgggt gaactgatcg cgcagcggcg tcagcagttg tttttcatcg ccaatccaca
    19981 tctgtgaaag aaagcctgac tggcggttaa attgccaacg cttattatcc agctcgatgc
    20041 aaaaatccgt ttcgctggtg gtcagttgcg ggatggcgtg gggcgcggag gggagtgtca
    20101 cgctgaggtt ttccgccaga cgccactgct gccaggcgct gatgtgtccg gcttctgacc
    20161 atgcggtcgc gttcggttgc actacgtgta ccgttagcca gagttgcccg gtgctctccg
    20221 gctgcggtag ttcaggcagt tcaattacct gtttaccttg tggagcgaca ttcataggta
    20281 cttcaccgct tgccagcggc ttgccatcca gtgccaccgt ccagtgcagg agctcgttat
    20341 cactatgacg gaacaggtat tcgctggtca cttcgatggt tcgcccggat aaacggaact
    20401 ggaaaaactg ctgctggtgt tttgcttccg tcagcgccgg atgcggcgtg cggtcggcaa
    20461 agaccagacc gttcatgcag aactggcgat cgttcggcgt atcgccaaaa tcaccgccgt
    20521 aagccgacca cgggttgccg ttttcatcat atttaatcag cgactgatcc acccagtccc
    20581 agacgaagcc gccctgtaaa cgggggtact gacgaaacgc ctgccagtat ttagcgaagc
    20641 cgccaagact gttacccatc gcgtgggcgt attcgcaaag gatcaacggg cgcgtctctc
    20701 caggcaacga aagccatttt ttgatggacc atttcggcac agccgggaag ggctggtctt
    20761 catccacgcg cgcgtacatc gggcaaataa tatcggttgc ggaggtgtcg gctccgccgc
    20821 cttcatactg caccgggcgg gatggatcga cagatttaat ccagcgatag agtgcgtcgt
    20881 gattagcgcc gtggcctgac tcattcccca gcgaccagat gatcacactc gggtgattac
    20941 gatcgcgctg caccattcgc gttacgcgtt cgctcatcgc gggtagccag cgcggatcat
    21001 cggtcagacg attcattggc accatgccgt gggtttcaat attggcttca tccaccacat
    21061 acaggccgta gcggtcgcac agcgtgtacc acagcggatg gttcggataa tgcgaacagc
    21121 gcacggcgtt aaagttattc tgcttcatca gcaggatatc ctgcaccatc gtctgctcat
    21181 ccatgacctg accatgtaga ggatgatgct cgtgacggtt aacgccgcga atcagcaacg
    21241 gcttgccgtt cagcagcagc aggccatttt caatccgtac ctcgcggaaa ccgacatcgc
    21301 aggcttctgc ttcaatcagc gtgccgtcgg cggtgtgcag ttcaaccacc gcacgataga
    21361 tattcgggat ttcggcgctc cacagtttcg ggttttcgac gttcagacct agtgtgacgc
    21421 gatcggcctc accgccacgc tcatcgataa tttcaccgcc gaaaggcgcg gtgccgctgg
    21481 cgacctgcgt ttcaccctgc cacaaagaaa ccgtcacccg cagctcatcg cgcagctcgc
    21541 cgtacatctg aacttctgcc tccagtaccg cgcggctgaa atcatcatta aagtgagtgg
    21601 caacatggaa atcgctgatt tgtgtggtcg gtttgtgcag caacgagacg tcacggaaaa
    21661 tgccgctcat ccgccacata tcctgatctt ccagataact gccgtcactc cagcgcagca
    21721 ccatcaccgc gaggcggttt tctccggcgc gtaaaaatgc gctcaggtca aattcagacg
    21781 gcaaacgact gtcctggccg taaccgaccc agcgcccgtt gcaccacaga tgaaacgccg
    21841 agttaacgcc atcaaaaata attcgcgtct ggccttcctg tagccagctt tcatcaacat
    21901 taaatgtgag cgagtaacaa cccgtcggat tctccgtggg aacaaacggc ggattgaccg
    21961 taatgggata ggtcacgttg gtgtagatgg gcgcatcgta accgtgcatc tgccagtttg
    22021 aggggacgac gacagtatcg gcctcaggaa gatcgcactc cagccagctt tccggcaccg
    22081 cttctggtgc cggaaaccag gcaaagcgcc attcgccatt caggctgcgc aactgttggg
    22141 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
    22201 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
    22261 ccagtgaatc cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
    22321 atttcacaca acatacgagc cggacgcata aagtgtaaag cctggggtgc ctaatgagtg
    22381 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
    22441 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
    22501 cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg
    22561 gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg
    22621 tttgatggtg gttaacggcg ggatataaca cgagctgtct tcggtatcgt cgtatcccac
    22681 tatcgagata tccgcaccaa cgcgcaaccc ggactcggta atggcgcgca ttgcgcccag
    22741 cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg
    22801 catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg
    22861 aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga
    22921 acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac
    22981 gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga
    23041 gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg
    23101 gtcatccagc ggatagttaa tgatcagccc acttacgcgt tgcgcgagaa gattgtgcac
    23161 cgccgcttta caggcttcga cgccgcttcg ttctaccatc gataccacca cgctggcacc
    23221 cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag
    23281 actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg
    23341 gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga
    23401 aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc
    23461 tgcgacatcg tatagcgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg
    23521 gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtcaa cgtaaatgca
    23581 cgccgcttcg ccttccggct accagaatag cctgcgattc aaccccttct tcgatctgtt
    23641 ttgctacccg ttgtagcgcc ggaagatgct tttccgcggc ctgttcaatg gtcattgcgc
    23701 tcgccatata caccagattc agacagccaa tcacccgttg ttcactgcgc agcggtacgg
    23761 cgatagaggc gatcttctcc tcctgatccc agccgcggta gttctgtccg taaccctctt
    23821 tgcgcgcgcg cgccagaatg gcttgcagct ttaacggttc tcgtgccagt tgatagtcat
    23881 caccggggcg ggaggctaac atctcgatta attccttgcg ttcttgttcc gggcaaaagg
    23941 ccagccaggt caggcccgag gcggttttca gaagcggcaa acgtcgcccg accattgccc
    24001 ggtgaaaaga taagcggctg aaacggtggg tggtttcgcg taccaccatt gcatcaacat
    24061 ccagcgtgga cacatctgtc ggccatacca cttcgcgcaa cagatcgccc agcagtgggg
    24121 ccgccagtgc agaaatccac tgttcgtcac gaaatccttc gcttaattgc cgcactttga
    24181 tggtcagtcg aaaactatca tcggaggggc tacggcggac atatccctct tcctgcagcg
    24241 tctccagcag tcgccgcaca gtggtgcgat gcaggccgct gagttccgcc agcagcccga
    24301 cgctggcacc gccatcaagt ttatttaaca tatttaataa cattagaccg cgggttaagc
    24361 cgcgcacggt tttgtattcc gtctgctcat tgttctgcat attaattgac atttctatag
    24421 ttaaaacaac gtggtgcacc tggtgcacat tcgggcatgt tttgattgta gccgaaaaca
    24481 cccttcctat actgagcgca caataaaaaa tcatttacat gtttttaaca aaataagttg
    24541 cgctgtactg tgcgcgcaac gacattttgt ccgagtcgtg aggtactgaa atggcaatac
    24601 aacaccctga catccagcct gctgttaacc atagcgttca ggtggcgatc gctggtgccg
    24661 gcccggttgg gctgatgatg gcgaactatc tcggccagat gggcattgac gtgctggtgg
    24721 tggagaaact cgataagttg atcgactacc cgcgtgcgat tggtattgat gacgaggcgc
    24781 tgcgcaccat gcagtcggtc ggcctggtcg atgatgttct gccgcacact acgccgtggc
    24841 acgcgatgcg ttttctcacc ccgaaaggcc gctgttttgc tgatattcag ccaatgaccg
    24901 atgaatttgg ctggccgcgc cgtaacgcct ttattcagcc gcaggtcgat gcggtgatgc
    24961 tggaaggggt gtcgcgtttt ccgaatgtgc gctgcttgtt ttcccgcgag ctggaggcct
    25021 tcagtcagca agatgacgaa gtgaccttgc acctgaaaac ggcagaaggg cagcgggaaa
    25081 tagtcaaagc ccagtggctg gtggcctgtg atggtggggc aagttttgtc cgtcgcaccc
    25141 tgaatgtgcc gtttgaaggt aaaactgcgc caaatcagtg gattgtggta gatatcgcca
    25201 acgatccgtt aagtacgccg catatctatt tgtgttgcga tccggtgcgc ccgtatgttt
    25261 ctgccgcgct acctcatgcg gtacgtcgct ttgaatttat ggtgatgcca ggagaaaccg
    25321 aagaacagct gcgtgagccg caaaatatgc gcaagctgtt aagcaaagtg ctgcctaatc
    25381 cggacaatgt tgaattgatt cgccagcgtg tctacaccca caacgcgcga ctggcgcaac
    25441 gtttccgtat tgatcgcgta ctgctggcgg gcgatgccgc gcacatcatg ccggtgtggc
    25501 aggggcaggg ctataacagt ggtatgcgcg acgcctttaa cctcgcatgg aaactggcgt
    25561 tggttatcca ggggaaagcc cgcgatgcgc tgctcaatac ctatcaacaa gaacgtcgcg
    25621 atcacgccaa agcgatgatt gacctgtccg tgacggcggg caacgtgctg gctccaccga
    25681 aacgctggca gggtacgtta cgtgacggcg tttcctggct gctgaattat ctgccgccag
    25741 taaaacgcta cttcctcgaa atgcgcttca agccgatgcc gcaatattac ggcggtgcgc
    25801 tggtacgtga gggcgaagcg aagcactctc cggtcggcaa gatgtttatt cagccgaaag
    25861 tcacgctgga aaacggcgac gtgacgctgt tcgataacgc gatcggcgcg aacttcgcgg
    25921 taattggctg gggatgcaat ccactgtggg ggatgagcga cgagcaaatc cagcagtggc
    25981 gcgcgttggg cacacgcttt attcaggtgg tgccggaagt gcaaattcat acggcacagg
    26041 ataaccacga cggcgtgcta cgcgtgggcg atacgcaagg tcgtctgcgt agctggttcg
    26101 cacaacacaa tgcttcgctg gtggtgatgc gcccggatcg ctttgttgcc gccaccgcca
    26161 ttccgcaaac cctgggcaag accctgaata aactggcgtc ggtgatgacg ctgacccgcc
    26221 ctgatgccga cgtttctgtc gaaaaggtag cctgatatgc acgcttatct tcattgtctt
    26281 tcccactcgc cgctggtggg gtatgtcgac ccggcacaag aggtactcga tgaggtcaat
    26341 ggcgtgattg ccagcgcccg cgagcgtatt gcggctttct cccctgaact ggtggtgctg
    26401 tttgcgccag atcactacaa cggctttttc tatgacgtga tgccaccgtt ctgtttaggc
    26461 gttggagcga cggcaattgg tgatttcggc agtgcggcag gagagctgcc cgtgcctgtg
    26521 gagctggcgg aggcctgtgc gcatgccgtc atgaagagcg ggatcgatct tgccgtttct
    26581 tactgtatgc aggtggacca cgggttcgcc cagccactgg agttcctgct cggcgggctg
    26641 gataaggtgc cagttctgcc tgtgttcatc aacggtgtcg ccacgccgct gcccggtttc
    26701 cagcgtaccc gcatgttggg tgaagccatt ggacgtttca ccagcactct caataaacgc
    26761 gtgctgttcc tgggttccgg tgggctttcc catcagccgc cggtgcccga actggcgaaa
    26821 gccgatgccc atatgcgcga ccgtctgttg gggagcggga aagatttacc cgccagtgag
    26881 cgcgaattgc gtcagcaacg ggtgattagc gccgctgaga agtttgttga ggatcagaga
    26941 acgctgcatc cgctcaaccc gatttgggat aaccagttca tgactttgct ggagcaggga
    27001 cgcatacagg aactggatgc cgtcagtaac gaagagcttt ccgccattgc cggaaagtcg
    27061 acacatgaaa tcaaaacctg ggtcgccgct tttgccgcta tttctgcgtt tggcaactgg
    27121 cgtagcgaag ggcgttatta ccgcccaatc ccggagtgga ttgccggatt tggctcgtta
    27181 agcgccagaa cagagaactg aatatgcagg agaagatgat gagttatcag ccacaaaccg
    27241 aagccgccac cagccgtttt ctgaatgtag aagaagcggg taaaacgctg cgcatccatt
    27301 ttaatgactg cggacaaggc gacgaaaccg ttgtcctgct gcatggttcc ggcccgggtg
    27361 ctactggctg ggcgaacttc agccgcaata tcgatccgct ggtagaggcg ggctatcggg
    27421 tgatcctgct ggattgtccg ggttggggca agagcgattc gatcgttaat agtggttcgc
    27481 gatcggatct taatgcacga atcctgaaaa gcgtggtgga tcaactggat atcgccaaaa
    27541 tccacctgct gggcaactcg atgggcggcc atagttctgt ggcgttcacc cttaactggc
    27601 cggagcgcgt cggcaaactg gtgctgatgg gcggcggtac gggcggcatg agtttgttta
    27661 cgccgatgcc aaccgaaggt attaagcgac tgaatcagct ttatcgtcag ccgactatcg
    27721 aaaacctgaa gctgatgatg gatatcttcg ttttcgatac cagcgatttg accgacgccc
    27781 tgtttgaagc gcgcctgaat aatatgctgt cgcgccgcga tcacctggaa aacttcgtta
    27841 agagcctgga agctaatccg aaacagttcc cggattttgg cccacgtctg gcggaaatca
    27901 aagcgcaaac cctgattgtc tgggggcgca acgaccgctt tgtgccgatg gatgcgggtc
    27961 tgcgtctgct gtccggcatt gccggttctg aactgcatat cttccgcgac tgtggtcact
    28021 gggcgcagtg ggaacatgcc gacgctttca atcaattggt gctgaatttc ctcgcacgcc
    28081 cttaaggaat ggtcatgacg aagcatactc ttgagcaact ggcggcggat ttacgccgcg
    28141 ccgcagagca gggcgaagcg attgcgccgc tgcgcgatct gattggtatc gataacgctg
    28201 aagcggccta cgccattcag cacataaatg tgcaatttga cgttgcgcag gggcgtcgcg
    28261 tggtggggcg taaagtgggc ctgacacatc cgaaagtgca gcaacaactg ggcgttgatc
    28321 aaccggattt cgggacatta tttgccgaca tgtgttatgg cgataacgaa atcattcctt
    28381 tttcccgtgt tctgcaaccc cgcattgaag cggagatcgc actggtgttg aaccgcgatt
    28441 tacccgcaac tgatatcacc ttcgacgaat tgtataacgc cattgaatgg gtacttccgg
    28501 cgctggaggt ggtggggagc cgcattcgcg actggtcgat tcagtttgtc gataccgtgg
    28561 cagataacgc ttcctgcggg gtgtatgtca tcggcggtcc ggcgcaacgt ccggcggggt
    28621 tagacctgaa aaactgcgcc atgaagatga cgcgtaataa cgaagaggtt tccagcgggc
    28681 gcggcagcga atgcctggga catccgctta atgccgccgt ctggctggca cgcaaaatgg
    28741 tcagcctggg tgaaccgctg tgcgccggag atatcattct taccggggca ttaggtccga
    28801 tggtggcggt gaatgcgggc gatcgttttg aagcccatat tgaaggcata ggttcagttg
    28861 ctgcgacatt ttcaagcgca gccccaaaag gaagtctgtc atgagtaagc gtaaagtcgc
    28921 cattatcggt tctggcaaca ttggcaccga tctgatgatt aaaattttgc gtcacggtca
    28981 gcatctggag atggcggtga tggtcggcat tgatcctcag tccgacggtc tggcgcgtgc
    29041 cagacgtatg ggcgtcgcca ccacccatga aggggtgatc gggctgatga acatgcctga
    29101 atttgctgat atcgacattg tatttgatgc gaccagcgcg ggcgcgcatg tgaaaaacga
    29161 tgccgcttta cgcgaagcga aaccggatat tcgcttaatt gacctgacgc ctgctgccat
    29221 cggcccttac tgcgtgccgg tggttaacct cgaggcgaac gtcgatcaac tgaacgtcaa
    29281 catggtcacc tgcggcggcc aggccaccat tccaatggtg gcggcagttt cacgcgtggc
    29341 gcgtgttcat tacgccgaaa ttatcgcttc tatcgccagt aaatctgccg gacctggcac
    29401 gcgtgccaat atcgatgaat ttacggaaac cacttcccga gccattgaag tggtgggcgg
    29461 cgcggcaaaa gggaaggcga ttattgtgct taacccagca gagccaccgt tgatgatgcg
    29521 tgacacggtg tatgtattga gcgacgaagc ttcacaagat gatatcgaag cctcaatcaa
    29581 tgaaatggct gaggcggtgc aggcttacgt accgggttat cgcctgaaac agcgcgtgca
    29641 gtttgaagtt atcccgcagg ataaaccggt caatttaccg ggcgtggggc aattctccgg
    29701 actgaaaaca gcggtctggc tggaagtcga aggcgcagcg cattatctgc ctgcctatgc
    29761 gggcaacctc gatattatga cttccagtgc gctggcgaca gcggaaaaaa tggctcagtc
    29821 actggcgcgc aaggcaggag aagcggcatg aacggtaaaa aactttatat ctcggacgtc
    29881 acgttgcgtg acggtatgca cgccattcgt catcagtatt cgctggaaaa cgttcgccag
    29941 attgccaaag cactggacga tgcccgcgtg gattcgattg aagtggccca tggcgacggt
    30001 ttgcaggggt ccagctttaa ctatggtttc ggcgcacata gcgaccttga atggattgaa
    30061 gcagcggcgg atgtggtgaa gcacgccaaa atcgcgacgt tgttgctgcc aggaatcggc
    30121 actattcacg atctgaaaaa tgcctggcag gctggcgcgc gggtggttcg tgtggctacg
    30181 cactgtaccg aagctgatgt ttccgcccag catattcagt atgcccgcga actcggaatg
    30241 gacaccgttg gttttctgat gatgagccat atgaccacgc cggagaatct cgccaagcag
    30301 gcaaagctga tggaaggcta cggtgcgacc tgtatttatg tggtggattc tggcggtgcg
    30361 atgaacatga gcgatatccg tgaccgtttc cgcgccctga aagcagtgct gaaaccagaa
    30421 acgcaaactg gcatgcacgc tcaccataac ctgagtcttg gcgtggcgaa ctctatcgcg
    30481 gcggtggaag agggctgcga ccgaatcgac gccagcctcg cgggaatggg cgcgggcgca
    30541 ggtaacgcgc cgctggaagt atttattgcc gccgtggata aactgggttg gcagcatggg
    30601 gccgatctct atgcgttaat ggatgccgct gacgacctgg tgcgtccgtt gcaggatcga
    30661 ccggtacgag tcgatcgcga aacgctggcg ctgggatacg ctggtgttta ctccagcttc
    30721 ctgcgtcact gtgaaacggc ggcggcgcgt tatggcttaa gcgcggtgga tattctcgtt
    30781 gagctgggca aacgccggat ggttggcggc caggaggata tgatcgtcga cgtggcgctg
    30841 gatctgcgcg acaacaaata atattgactg ccgagaacgt gcattttgta tgcccatccc
    30901 agcacgactg ccggatgcgg cgtgaacgcc ttatccggcc tacggatggc gcgagaattt
    30961 gtaggcctga taagacgcgt tagcgtcgca tcaggcatct gcgcacgact gccagatgcg
    31021 gcgcaaacgt cttgtccggc ctacggatgg cgcgggaatt tgtaggcctg ataagacgcg
    31081 t
//
LOCUS       DAPGOF010000046        30723 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.046, whole genome shotgun sequence.
ACCESSION   DAPGOF010000046 DAPGOF010000000
VERSION     DAPGOF010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30723
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.046"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            245..628
                     /gene="secE"
                     /locus_tag="RHV13_001771"
     CDS             245..628
                     /gene="secE"
                     /locus_tag="RHV13_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDV1241311.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            630..1175
                     /gene="nusG"
                     /locus_tag="RHV13_001772"
     CDS             630..1175
                     /gene="nusG"
                     /locus_tag="RHV13_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDV1241312.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1334..1762
                     /gene="rplK"
                     /locus_tag="RHV13_001773"
     CDS             1334..1762
                     /gene="rplK"
                     /locus_tag="RHV13_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDV1241313.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1766..2470
                     /gene="rplA"
                     /locus_tag="RHV13_001774"
     CDS             1766..2470
                     /gene="rplA"
                     /locus_tag="RHV13_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDV1241314.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2762..3259
                     /gene="rplJ"
                     /locus_tag="RHV13_001775"
     CDS             2762..3259
                     /gene="rplJ"
                     /locus_tag="RHV13_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDV1241315.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3326..3691
                     /gene="rplL"
                     /locus_tag="RHV13_001776"
     CDS             3326..3691
                     /gene="rplL"
                     /locus_tag="RHV13_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDV1241316.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4011..8039
                     /gene="rpoB"
                     /locus_tag="RHV13_001777"
     CDS             4011..8039
                     /gene="rpoB"
                     /locus_tag="RHV13_001777"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDV1241317.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8116..12339
                     /gene="rpoC"
                     /locus_tag="RHV13_001778"
     CDS             8116..12339
                     /gene="rpoC"
                     /locus_tag="RHV13_001778"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDV1241318.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            12552..13091
                     /locus_tag="RHV13_001779"
     CDS             12552..13091
                     /locus_tag="RHV13_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-shock protein C"
                     /protein_id="HDV1241319.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(13501..14634)
                     /gene="thiH"
                     /locus_tag="RHV13_001780"
     CDS             complement(13501..14634)
                     /gene="thiH"
                     /locus_tag="RHV13_001780"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HDV1241320.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14631..15401)
                     /gene="thiG"
                     /locus_tag="RHV13_001781"
     CDS             complement(14631..15401)
                     /gene="thiG"
                     /locus_tag="RHV13_001781"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HDV1241321.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15403..15603)
                     /gene="thiS"
                     /locus_tag="RHV13_001782"
     CDS             complement(15403..15603)
                     /gene="thiS"
                     /locus_tag="RHV13_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HDV1241322.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINHQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15587..16342)
                     /gene="thiF"
                     /locus_tag="RHV13_001783"
     CDS             complement(15587..16342)
                     /gene="thiF"
                     /locus_tag="RHV13_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HDV1241323.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16335..16970)
                     /gene="thiE"
                     /locus_tag="RHV13_001784"
     CDS             complement(16335..16970)
                     /gene="thiE"
                     /locus_tag="RHV13_001784"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDV1241324.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16970..18865)
                     /gene="thiC"
                     /locus_tag="RHV13_001785"
     CDS             complement(16970..18865)
                     /gene="thiC"
                     /locus_tag="RHV13_001785"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HDV1241325.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTQPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGLFTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18946..19046)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19099..19575)
                     /gene="rsd"
                     /locus_tag="RHV13_001786"
     CDS             complement(19099..19575)
                     /gene="rsd"
                     /locus_tag="RHV13_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HDV1241326.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19670..20443
                     /gene="nudC"
                     /locus_tag="RHV13_001787"
     CDS             19670..20443
                     /gene="nudC"
                     /locus_tag="RHV13_001787"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HDV1241327.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELFEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20483..21547
                     /gene="hemE"
                     /locus_tag="RHV13_001788"
     CDS             20483..21547
                     /gene="hemE"
                     /locus_tag="RHV13_001788"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HDV1241328.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSAPYHQ"
     gene            21557..22228
                     /gene="nfi"
                     /locus_tag="RHV13_001789"
     CDS             21557..22228
                     /gene="nfi"
                     /locus_tag="RHV13_001789"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HDV1241329.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPTLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22271..22861
                     /locus_tag="RHV13_001790"
     CDS             22271..22861
                     /locus_tag="RHV13_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HDV1241330.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            23048..23320
                     /gene="hupA"
                     /locus_tag="RHV13_001791"
     CDS             23048..23320
                     /gene="hupA"
                     /locus_tag="RHV13_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HDV1241331.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23333..24028
                     /locus_tag="RHV13_001792"
     CDS             23333..24028
                     /locus_tag="RHV13_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HDV1241332.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24030..24455)
                     /gene="zraP"
                     /locus_tag="RHV13_001793"
     CDS             complement(24030..24455)
                     /gene="zraP"
                     /locus_tag="RHV13_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HDV1241333.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24693..26069
                     /gene="zraS"
                     /locus_tag="RHV13_001794"
     CDS             24693..26069
                     /gene="zraS"
                     /locus_tag="RHV13_001794"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HDV1241334.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLLEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQHEAIFTPYFTTKAEGTGLGLTVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            26066..27391
                     /gene="zraR"
                     /locus_tag="RHV13_001795"
     CDS             26066..27391
                     /gene="zraR"
                     /locus_tag="RHV13_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HDV1241335.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIAGTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27388..28677)
                     /gene="purD"
                     /locus_tag="RHV13_001796"
     CDS             complement(27388..28677)
                     /gene="purD"
                     /locus_tag="RHV13_001796"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDV1241336.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDDVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACESKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADDEQVVTNGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28689..30278)
                     /gene="purH"
                     /locus_tag="RHV13_001797"
     CDS             complement(28689..30278)
                     /gene="purH"
                     /locus_tag="RHV13_001797"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDV1241337.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDVIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPSLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7241 a         7955 c         8404 g         7123 t
ORIGIN      
        1 aaagttctga gctaattgcc gataacattt gacgcaatgc gcactaaaag ggcatcattt
       61 gatgcccttt ttgcacgctt tcgtaccaga acctggctca tcagtgattt tctttgtcat
      121 aatcattgct gagacaggct ctgttgaggg cgtataatcc gaaaagctaa tacgcgtttc
      181 gatttggttt gcctcgcgat cgcggggtga aaatgtttgt agaatacttc tgacaggttg
      241 gtttatgagt gcgaataccg aagctcaagg aagcgggcgc ggcctggaag cgatgaagtg
      301 ggtcgttgtg gtggcattgc tcctggtggc gattgtcggc aactaccttt atcgcgacat
      361 tatgctgccg ctgcgtgcgc tggccgtagt aattctgatt gctgcagcgg gtggtgtcgc
      421 gctgttaacg acaaaaggta aagctaccgt tgcttttgcc cgtgaagcgc gtaccgaagt
      481 ccgtaaggtc atttggccga ctcgccagga aacattgcac accacgctga ttgtggctgc
      541 ggttaccgca gtaatgtcac tgatcctgtg gggactggat ggtattctgg ttcgcctggt
      601 atcctttatc actggcctga ggttctgaga tgtctgaagc tcctaaaaag cgctggtacg
      661 tcgttcaggc gttttccggt tttgaaggcc gcgtagcaac gtcgctgcgt gagcatatca
      721 aattacacaa catggaagat ttgtttggtg aagtcatggt accaaccgaa gaggtggttg
      781 aaatccgtgg cggtcagcgt cgcaaaagcg aacgtaaatt cttccctggc tacgtcctcg
      841 ttcagatggt gatgaacgac gcgagctggc acctggtgcg cagcgtaccg cgtgtgatgg
      901 gcttcatcgg cggtacttcc gatcgtcctg cgccaatcag cgataaagaa gtcgatgcga
      961 ttatgaaccg cctgcagcag gttggtgata agccgcgtcc gaaaacgctg tttgaaccgg
     1021 gtgaaatggt ccgtgttaat gatggtccgt tcgctgactt caacggtgtt gttgaagaag
     1081 tggattacga gaaatctcgt ctgaaagtgt ctgtttctat cttcggtcgt gcgaccccgg
     1141 tagagctgga cttcagccag gttgaaaaag cctaacccag cgatcaaaaa agcgacgatt
     1201 taatcgttgc acaaggcgcg agattggaat acaatttcgc gccttttgtt tttatgggtc
     1261 ttgcccgtaa aacgattttt tatatcacgg ggagcctctc agaggcgtta ttacccaact
     1321 tgaggaattt ataatggcta agaaagtaca agcctatgtc aagctgcagg ttgcagctgg
     1381 tatggctaac ccgagtccgc cagtaggtcc ggctctgggt cagcagggcg taaacatcat
     1441 ggaattctgc aaagcgttca acgcaaaaac tgattccatc gaaaaaggtc tgccgattcc
     1501 ggtagtaatc accgtttacg ctgaccgttc tttcactttc gttaccaaga ctccgccggc
     1561 agcagttctg ctgaaaaaag cggctggtat caagtctggt tccggtaagc cgaacaaaga
     1621 caaagtgggt aaaatttccc gcgctcagct gcaggaaatc gcgcagacca aagctgccga
     1681 catgactggt gccgacattg aagcgatgac tcgctccatc gaaggtactg cacgttccat
     1741 gggcctggta gtggaggact aagaaatggc taaactgacc aagcgcatgc gtgttatccg
     1801 cgagaaagtt gatgcaacca aacagtacga catcaacgaa gctatcgctc tgctgaaaga
     1861 gctggcgact gctaaattcg tagaaagcgt ggacgtagct gttaacctcg gcatcgacgc
     1921 tcgtaaatct gaccagaacg tacgtggtgc aactgtactg ccgcacggta ctggccgttc
     1981 cgttcgcgta gccgtattta cccagggtgc aaacgctgaa gctgctaaag ctgcaggtgc
     2041 tgaactggta ggtatggaag atctggctga ccagatcaag aaaggcgaaa tgaactttga
     2101 cgttgttatt gcttctccgg atgcaatgcg cgttgttggc cagctgggcc aggttctggg
     2161 cccgcgcggc ctgatgccaa acccgaaagt gggtactgta acgccgaacg ttgctgaagc
     2221 ggttaaaaac gctaaagctg gccaggttcg ttaccgtaac gacaaaaacg gcatcatcca
     2281 caccaccatc ggtaaagtgg actttgacgc tgacaaactg aaagaaaacc tggaagctct
     2341 gctggttgcg ctgaaaaaag caaaaccgac tcaggcgaaa ggcgtgtaca tcaagaaagt
     2401 tagcatctcc accaccatgg gtgcaggtgt tgcagttgac caggctggcc tgagcgcttc
     2461 tgtaaactaa tgcctttacg tgggcggtga ttttgtctac aatcttaccc ccacgtatgc
     2521 taacgaaagt tagcagctaa aaagatttgt tcgttggagc ctggcctatc caggcctccg
     2581 tcgaagaccg caggagtttc gcaagaaact taatcccctg cgtagacggt gacagaacgc
     2641 taagattatt cttttatatt ctggcttgtt tctgctcacc gtaattaaga cgctctctcc
     2701 gtttggagga gtgaagtgag ttccagagat tttctctggc aaacatccag gagcaaagct
     2761 aatggcttta aatcttcaag acaaacaagc gattgttgct gaagtcagcg aagtagccaa
     2821 aggcgcgctg tctgcagtag ttgcggattc ccgtggcgta actgtagata aaatgactga
     2881 actgcgtaaa gcaggtcgcg aagctggcgt atacatgcgt gttgttcgta acaccctgct
     2941 gcgccgtgct gttgaaggta ctccgttcga gtgcctgaaa gacgcgtttg ttggtccgac
     3001 cctgattgca tactctatgg aacacccggg cgctgctgct cgtctgttca aagagttcgc
     3061 gaaagcgaat gcaaaatttg aggtcaaagc cgctgccttt gaaggtgagc tgatcccggc
     3121 gtctcagatc gaccgcctgg caactctgcc gacctacgaa gaagcaattg cacgcctgat
     3181 ggcaaccatg aaagaagctt cggctggcaa actggttcgt actctggctg ctgtacgcga
     3241 tgcgaaagaa gctgcttaat cgcagttatc tttttaacgc attcgcttac gtataaactt
     3301 attctgatat tcaggaacaa tttaaatgtc tatcactaaa gatcaaatca ttgaagcagt
     3361 tgcagctatg tctgtaatgg acgttgtaga actgatctct gcaatggaag aaaaattcgg
     3421 tgtttccgct gctgctgctg tagctgtagc tgctggcccg gttgaagctg ctgaagaaaa
     3481 aactgaattc gacgtaattc tgaaagctgc tggcgctaac aaagttgctg ttatcaaagc
     3541 agtacgtggc gcaactggcc tgggtctgaa agaagctaaa gacctggtag aatctgcacc
     3601 ggctgctctg aaagaaggcg tgagcaaaga cgacgcagaa gcactgaaaa aagctctgga
     3661 agaagctggc gctgaagttg aagttaaata agccaaccct tccggttgca gcctgagaaa
     3721 tcaggctgat ggctggtgac tttttagtca ccagcctttt tgcgctgtaa ggcgccagta
     3781 gcgtttcaca ctgtttgact actgctgtgc ctttcaatgc ttgtttctat cgacgactta
     3841 atatactgcg acaggacgtc cgttctgtgt aaatcgcaat gaaatggttt aagcgtgata
     3901 gcaacaggca ttgcggaaag tgttccattt tccggtcaac aaaatagtgt tgcacaaact
     3961 gtccgctcaa tggacagatg ggtcgacttg tcagcgagct gaggaaccct atggtttact
     4021 cctataccga gaaaaaacgt attcgtaagg attttggtaa acgtccacaa gttctggatg
     4081 taccttatct cctttctatc cagcttgact cgtttcagaa atttatcgag caagatcctg
     4141 aagggcagta tggtctggaa gctgctttcc gttccgtatt cccgattcag agctacagcg
     4201 gtaattccga gctgcaatac gtcagctacc gccttggcga accggtgttt gacgtccagg
     4261 aatgtcaaat ccgtggcgtg acctattccg caccgctgcg cgttaaactg cgtctggtga
     4321 tctatgagcg cgaagcgccg gaaggcaccg taaaagacat taaagaacaa gaagtctaca
     4381 tgggcgaaat tccgctcatg acagacaacg gtacctttgt tatcaacggt actgagcgtg
     4441 ttatcgtttc ccagctgcac cgtagtccgg gcgtcttctt tgactccgac aaaggtaaaa
     4501 cccactcttc gggtaaagtg ctgtataacg cgcgcatcat cccttaccgt ggttcttggc
     4561 tggacttcga attcgatccg aaggacaacc tgttcgtacg tatcgaccgt cgccgtaaac
     4621 tgcctgcgac catcattctg cgtgccctga actacaccac agagcagatc ctcgacctgt
     4681 tctttgaaaa agttatcttt gaaatccgtg ataacaagct gcagatggaa ctggtgccgg
     4741 aacgcctgcg tggtgaaacc gcatcttttg acatcgaagc taacggtaaa gtgtacgtag
     4801 aaaaaggccg ccgtattact gcgcgccaca ttcgccagct ggaaaaagac gacgtcaaac
     4861 tgatcgaagt cccggttgag tacatcgcag gtaaagtggt tgctaaagac tatattgatg
     4921 agtctaccgg cgagctgatc tgcgcagcga acatggagct gagcctggat ctgctggcta
     4981 agctgagcca gtctggtcac aagcgtatcg aaacgctgtt caccaacgat ctggatcacg
     5041 gcccatatat ctctgaaacc ttacgtgtcg acccaactaa cgaccgtctg agcgcactgg
     5101 tagaaatcta ccgcatgatg cgccctggcg agccgccgac tcgtgaagca gcggaaagcc
     5161 tgttcgagaa cctgttcttc tccgaagacc gttatgacct gtctgcggtt ggtcgtatga
     5221 agttcaaccg ttctctgctg cgcgaagaaa tcgaaggttc cggtatcctg agcaaagacg
     5281 acatcattga tgttatgaaa aagctcatcg atatccgtaa cggtaaaggc gaagtcgatg
     5341 atatcgacca cctcggcaac cgtcgtatcc gttccgttgg cgaaatggcg gaaaaccagt
     5401 tccgcgttgg tctggtacgt gtagagcgtg cggtgaaaga gcgtctgtct ctgggcgatc
     5461 tggataccct gatgcctcag gatatgatca acgccaagcc gatttccgca gcagtgaaag
     5521 agttcttcgg ttccagccag ctgtctcagt ttatggacca gaacaacccg ctgtctgaga
     5581 ttacgcacaa acgtcgtatc tccgcactcg gcccaggcgg tctgacccgt gaacgtgcag
     5641 gcttcgaagt tcgagacgta cacccgactc actacggtcg cgtatgtcca atcgaaaccc
     5701 ctgaaggtcc gaacatcggt ctgatcaact ctctgtccgt gtacgcacag actaacgaat
     5761 acggcttcct tgagactccg tatcgtaaag tgactgacgg tgttgtaact gacgaaattc
     5821 actacctgtc tgctatcgaa gaaggcaact acgttatcgc ccaggcgaac tccaacctgg
     5881 atgaagaagg ccacttcgta gaagacctgg taacttgccg tagcaaaggc gaatccagct
     5941 tgttcagccg cgaccaggtc gactacatgg acgtatccac ccagcaggtg gtatccgtcg
     6001 gtgcgtccct gatcccgttc ctggaacacg atgacgccaa ccgtgcattg atgggtgcga
     6061 acatgcaacg tcaggccgtt ccgactctgc gcgctgataa gccgctggtt ggtactggta
     6121 tggaacgtgc tgttgccgtt gactccggtg taactgcggt agctaaacgt ggtggtgtcg
     6181 ttcagtacgt ggatgcttcc cgtatcgtta tcaaagttaa cgaagacgag atgtatccgg
     6241 gtgaagcagg tatcgacatc tacaacctga ccaaatacac ccgttctaac cagaacacct
     6301 gtatcaacca gatgccgtgt gtgtctctgg gtgaaccggt tgaacgtggc gacgtgctgg
     6361 cagatggtcc gtccaccgac ctcggtgaac tggcgcttgg tcagaacatg cgcgtagcgt
     6421 tcatgccgtg gaatggttac aacttcgaag actccatcct cgtatccgag cgtgttgttc
     6481 aggaagaccg tttcaccacc atccacattc aggaactggc gtgtgtgtcc cgtgacacca
     6541 agctggggcc ggaagagatc accgctgaca tcccgaacgt gggtgaagct gcgctctcca
     6601 aactggatga atccggtatc gtttacattg gtgcggaagt gaccggtggc gacattctgg
     6661 ttggtaaggt aacgccgaaa ggtgaaactc agctgacccc agaagaaaaa ctgctgcgtg
     6721 cgatcttcgg tgagaaagcc tctgacgtta aagactcttc tctgcgcgta ccaaacggtg
     6781 tatccggtac ggttatcgac gttcaggtct ttactcgcga tggcgtagaa aaagacaaac
     6841 gtgcgctgga aatcgaagaa atgcagctca aacaggcgaa gaaagacctg tctgaagaac
     6901 tgcagatcct cgaagcgggt ctgttcagcc gtatccgtgc tgtgctggta gccggtggcg
     6961 ttgaagctga gaagctcgac aaactgccgc gcgatcgctg gctggagctg ggcctgacag
     7021 acgaagagaa acaaaatcag ctggaacagc tggctgagca gtatgacgaa ctgaaacacg
     7081 agttcgagaa gaaactcgaa gcgaaacgcc gcaaaatcac ccagggcgac gatctggcac
     7141 cgggcgtgct gaagattgtt aaggtatatc tggcggttaa acgccgtatc cagcctggtg
     7201 acaagatggc aggtcgtcac ggtaacaagg gtgtaatttc taagatcaac ccgatcgaag
     7261 atatgcctta cgatgaaaac ggtacgccgg tagacatcgt actgaacccg ctgggcgtac
     7321 cgtctcgtat gaacatcggt cagatcctcg aaacccactt gggtatggct gcgaaaggta
     7381 tcggcgacaa gatcaacgcc atgctgaaac agcagcagga agtcgcgaaa ctgcgcgaat
     7441 tcatccagcg tgcgtacgat ctgggcgctg acgttcgtca gaaagttgac ctgagtacct
     7501 tcagcgatga agaagttatg cgtctggctg aaaacctgcg caaaggtatg ccaatcgcaa
     7561 cgccggtgtt cgacggtgcg aaagaagcag aaattaaaga gctgctgaaa cttggcgacc
     7621 tgccgacttc tggtcagatc cgcctgtacg acggccgcac tggtgaacag ttcgaacgtc
     7681 cggtaaccgt tggttacatg tacatgctga aactgaacca cctggtcgac gacaagatgc
     7741 acgcgcgttc caccggttct tacagcctgg ttactcagca gccgctgggt ggtaaggcac
     7801 agttcggtgg tcagcgtttc ggggagatgg aagtgtgggc gctggaagca tacggcgcag
     7861 catacaccct gcaggaaatg ctcaccgtta agtctgatga cgtgaacggt cgtaccaaga
     7921 tgtataaaaa catcgtggac ggcaaccatc agatggagcc gggcatgcca gaatccttca
     7981 acgtattgtt gaaagagatt cgttcgctgg gtatcaacat cgaactggaa gacgagtaat
     8041 tctcgctcaa acaggtcact gctgtcgggt taaaacccgg cagcggattg tgctaactcc
     8101 gacgggagca aatccgtgaa agatttatta aagtttctga aagcgcagac taaaaccgaa
     8161 gagtttgatg cgatcaaaat tgctctggct tcgccagaca tgatccgttc atggtctttc
     8221 ggtgaagtta aaaagccgga aaccatcaac taccgtacgt tcaaaccaga acgtgacggc
     8281 cttttctgcg cccgtatctt tgggccggta aaagattacg agtgcctgtg cggtaagtac
     8341 aagcgcctga aacaccgtgg cgtcatctgt gagaagtgcg gcgttgaagt gacccagact
     8401 aaagtacgcc gtgagcgtat gggccacatc gaactggctt ccccgactgc gcacatctgg
     8461 ttcctgaaat cgctgccgtc ccgtatcggt ctgctgctcg atatgccgct gcgcgatatc
     8521 gaacgcgtac tgtactttga atcctatgtg gttatcgaag gcggtatgac caacctggaa
     8581 cgtcagcaga tcctgactga agagcagtat ctggacgcgc tggaagagtt cggtgacgaa
     8641 ttcgacgcga agatgggggc ggaagcaatc caggctctgc tgaagagcat ggatctggag
     8701 caagagtgcg aacagctgcg tgaagagctg aacgaaacca actccgaaac caagcgtaaa
     8761 aagctgacca agcgtatcaa actgctggaa gcgttcgttc agtctggtaa caaaccagag
     8821 tggatgatcc tgaccgttct gccggtactg ccgccagatc tgcgtccgct ggttccgctg
     8881 gatggtggtc gtttcgcgac ttctgacctg aacgatctgt atcgtcgcgt cattaaccgt
     8941 aacaaccgtc tgaaacgtct gctggatctg gctgcgccgg acatcatcgt acgtaacgaa
     9001 aaacgtatgc tgcaggaagc ggtagacgcc ctgctggata acggtcgtcg cggtcgtgcg
     9061 atcaccggtt ctaacaagcg tcctctgaaa tctttggccg acatgatcaa aggtaaacag
     9121 ggtcgtttcc gtcagaacct gctcggtaag cgtgttgact actccggtcg ttctgtaatc
     9181 accgtaggtc catacctgcg tctgcatcag tgcggtctgc cgaagaaaat ggcgctggag
     9241 ctgttcaaac cgttcatcta cggcaagctg gaactgcgtg gtcttgctac caccattaaa
     9301 gctgcgaaga aaatggttga gcgcgaagaa gctgtcgttt gggatatcct ggacgaagtt
     9361 atccgcgaac acccggtact gctgaaccgt gcaccgactc tgcaccgtct gggtatccag
     9421 gcatttgaac cggtactgat cgaaggtaaa gctatccagc tgcacccgct ggtttgtgcg
     9481 gcatataacg ccgacttcga tggtgaccag atggctgttc acgtaccgct gacgctggaa
     9541 gcccagctgg aagcgcgtgc gctgatgatg tctaccaaca acatcctgtc cccggcgaac
     9601 ggcgaaccaa tcatcgttcc gtctcaggac gttgtactgg gtctgtacta catgacccgt
     9661 gactgtgtta acgccaaagg cgaaggcatg gtgctgactg gcccgaaaga agcagaacgt
     9721 ctgtatcgct ctggtctggc ttctctgcat gcgcgcgtta aagtgcgtat caccgagtat
     9781 gaaaaagatg ctaacggtga attagtagcg aaaaccagcc tgaaagacac gactgttggc
     9841 cgtgccattc tgtggatgat tgtaccgaaa ggtctgcctt actccatcgt caaccaggcg
     9901 ctgggtaaaa aagcaatctc caaaatgctg aacacctgct accgcattct cggtctgaaa
     9961 ccgaccgtta tttttgcgga ccagatcatg tacaccggct ttgcctatgc agcgcgttct
    10021 ggtgcatctg ttggtatcga tgacatggtc atcccggaga agaaacacga aatcatctcc
    10081 gaggcagaag cagaagttgc tgaaattcag gagcagttcc agtctggtct ggtaactgcg
    10141 ggcgaacgct acaacaaagt tatcgatatc tgggctgcgg cgaacgatcg tgtatccaaa
    10201 gcgatgatgg ataacctgca aactgaaacc gtgattaacc gtgacggtca ggaagagaag
    10261 caggtttcct tcaacagcat ctacatgatg gccgactccg gtgcgcgtgg ttctgcggca
    10321 cagattcgtc agcttgctgg tatgcgtggt ctgatggcga agccggatgg ctccatcatc
    10381 gaaacgccaa tcaccgcgaa cttccgtgaa ggtctgaacg tactccagta cttcatctcc
    10441 acccacggtg ctcgtaaagg tctggcggat accgcactga aaacggcgaa ctccggttac
    10501 ctgactcgtc gtctggttga cgtggcgcag gacttggtgg ttaccgaaga cgattgtggt
    10561 acccatgaag gtatcatgat gactccggtt atcgaaggtg gtgacgttaa agagccgctg
    10621 cgcgatcgcg tactgggtcg tgtaactgct gaagacgttc tgaagccggg tactgctgat
    10681 atcctcgttc cgcgcaacac gctgctgcac gaacagtggt gtgacctgct ggaagagaac
    10741 tctgtcgacg cggtaaaagt acgttctgtt gtatcttgtg acaccgactt tggtgtatgt
    10801 gcgcactgct acggtcgtga cctggcgcgt ggccacatca tcaacaaagg tgaagcaatc
    10861 ggtgttatcg cggcacagtc catcggtgaa ccgggtacac agctgaccat gcgtacgttc
    10921 cacatcggtg gtgcggcatc tcgtgcggct gctgaatcca gcatccaggt gaaaaacaaa
    10981 ggtagcatca agctcagcaa cgtgaagtcg gttgtgaact ccagcggtaa actggttatc
    11041 acttcccgta acaccgaact gaaactgatc gacgaattcg gtcgtaccaa agaaagctac
    11101 aaagtgcctt acggtgcggt actggcgaaa ggcgatggcg aacaggttgc tggcggcgaa
    11161 accgttgcaa actgggaccc gcacaccatg ccggttatca ccgaagtaag cggttttgta
    11221 cgctttactg acatgatcga cggccagacc attactcgtc agaccgacga actgaccggt
    11281 ctgtcttcgc tggtggttct ggattccgca gaacgtaccg caggtggtaa agatctgcgt
    11341 ccggcactga aaatcgttga tgctcagggt aacgacgttc tgatccctgg taccgatatg
    11401 cctgcgcagt acttcctgcc gggtaaagcg attgttcagc tggaagatgg cgtacagatc
    11461 agctctggtg acaccttggc gcgtattccg caggaatccg gcggtaccaa ggacatcacc
    11521 ggtggtctgc cgcgcgttgc ggacctgttc gaagcacgtc gtccgaaaga gccggcaatc
    11581 ctggctgaaa tcagcggtat cgtttccttc ggtaaagaaa ccaaaggtaa acgtcgtctg
    11641 gttatcaccc cggtagacgg tagcgatccg tacgaagaga tgattccgaa atggcgtcag
    11701 ctcaacgtgt tcgaaggtga acgtgtagaa cgtggtgacg taatttccga cggtccggaa
    11761 gcgccgcacg acattctgcg tctgcgtggt gttcatgctg ttactcgtta catcgttaac
    11821 gaagtacagg acgtataccg tctgcagggc gttaagatta acgataaaca catcgaagtt
    11881 atcgttcgtc agatgctgcg taaagctacc atcgttaacg cgggcagctc cgacttcctg
    11941 gaaggcgaac aggttgaata ctctcgcgtc aagatcgcaa accgcgaact ggaagcgaac
    12001 ggcaaagtgg gtgcaactta ctcccgcgat ctgctgggta tcaccaaagc gtctctggca
    12061 accgagtcct tcatctccgc ggcatcgttc caggagacca ctcgcgtgct gaccgaagca
    12121 gccgttgcgg gcaaacgcga cgaactgcgc ggcctgaaag agaacgttat cgtgggtcgt
    12181 ctgatcccgg caggtaccgg ttacgcgtac caccaggatc gtatgcgtcg ccgtgctgcg
    12241 ggtgaagctc cggctgcacc gcaggtgact gcagaagacg catctgccag cctggcagaa
    12301 ctgctgaacg caggtctggg cggttctgat aacgagtaat cgttaatccg caaataacgt
    12361 aaaaacccgc ttcggcgggt ttttttatgg ggggagttta gggaaaaagc atttgtcaga
    12421 atatttaagg aatttctgaa tactcataat caatgtagag atgactaata tcctgaaact
    12481 gactgaacta attgagtcaa actcggcaag gattcgatac tattcctgtg taactttctt
    12541 aaggaacgag aatgaaacag gaagtggaaa agtggcgacc ttttggacat ccggatggtg
    12601 atattcgtga tttatcattt cttgatgctc atcaggctgt ctacgttcag catcatgagg
    12661 gcaaagagcc tttagagtat cgcttttggg ttacctactc tcttcactgc ttcacaaaag
    12721 attatgaaca tcagacgaac gaagaaaaac aatcgttaat gtaccacgcg cctaaagaat
    12781 ctcgtccctt ctgccagcac cgttataact tagcgcgcac acacttaaaa agaactattt
    12841 tggcgctgcc agaaagcaac gttattcatg ccgggtatgg tagctatgcc gtgattgagg
    12901 tggacttaga cggaggagat aaggcatttt actttgttgc gttcagggct ttcagggaaa
    12961 agaaaaaact ccgtttgcat gtaactagcg cttatcccat ttctgaaaaa cagaaaggta
    13021 aatcagtgaa atttttcacc attgcctaca acttattgag aaataagcag cttcctcagc
    13081 cctcaaaata acaaaaccca ccttaaggtg ggtttcgcca gagaattatc tctggtattc
    13141 agaacgccat taccggactt tgccttgacc ttgcgataat cgcaggttgc gggatgtctg
    13201 aatttcttca gtctgctgca tcctggaaga tgagaacatg tgttcttatt ttcgtctcta
    13261 tcatagttga gtatttactc tcttacaatc agatctcttt cattgctcaa caggcgatgg
    13321 cttcagactt tgcattacgg aatttttaag aaaggcaggg cgaaacgagg aagaagcttt
    13381 tcgccctgga caacattcct gctgacggca ctaccataaa aaaacacaac gctttcaaca
    13441 accttgcgtt tttcatcttc cgtagcggct cggaataaat ttacgttctt acattccgtc
    13501 tcatagtctt tgcgaggcgc gtcccagata gctgtcccag tctttccata ccggctgcaa
    13561 accctgagcg gttaacgcgg cagcaaccgc ttccggtctg cgatcgtcgt gcggtgagaa
    13621 ctgttccagc tcggggtgat tatcggcata gccacctggc tgcgttttcg agaaggcgct
    13681 gacgttattg atcgccagcg gaataacgcg atcgcgaaac cacggtgatt cccgcgtgga
    13741 gagtgacagt tcaatctccg gtgcaagcag tcggaaggcg cagatggttt gcactaactg
    13801 gcgttcatcc ataatcgacg caggctcaat gccgccagta cacgggcgca ggcgcggaaa
    13861 ggagacagag taacggcttt gccagtaatg ctgttgcagc catagcaaat gttcggcaac
    13921 catatagcag tcaacgcgcc agttgtcgga aaggccaatt agcgcgccga ggcctatctt
    13981 atcaatcccc gcacgcccca gccgatccgg cgtttccagc cgccagaaga agtcctgttt
    14041 tttgcctttc agatgatggc gggcataagt cgcctcgtga tatgtctcct gataaaccat
    14101 cacgccatcc agaccaagct gctttaactc ggcgtattcc gtctccgcca gcggttgcac
    14161 ttccatttgt agtgaagaga actgttcacg cagggcgggg agatgacgac gaaagtaatc
    14221 catccccact ttcgcctgat gttcaccagt gactaaaagc agatgttcaa agcccatctc
    14281 ccgtatagcg gcgctttccc tggcaatatc cgcttcgtcc agcgttttgc gcttgatgcg
    14341 attactcatg gaaaatccgc agtacgtgca gtcgttagcg caaagattgg aaagataaag
    14401 cgggacgtag aaactaactg tgttgccaaa tcgctgacgg gtcagacgct gcgcccgttg
    14461 ggccagttgt tccagatagc cactggcggc tggcgataac agcgccatca tatcgtcgcg
    14521 ggtgagttgc gaggcattta gcgcccgttc tacgtcagca gccgttttgc cgttgatacg
    14581 caggcggatg tcgtcccagt ccagttgtcg ccagcgatcg ctgaaggttt tcatgccgat
    14641 gcctccagaa atccagtcag cgggctggtg gcatgagcaa aatgactgcg gctgcccggt
    14701 ccggactgac gtgccagcag gcctgcttct accgccagac gaaatgcctt cgccatgttg
    14761 acgggatcgt ccgcgacggc aattgccgta ttcactaaca ccgcgtcggc ccccatttcc
    14821 agcgcctgcg cggcatggct gggaacgccg atgccagcat cgacaaccac cggcactgtg
    14881 gcctgctgga taataatctc cagcatggcg cgggtttcca gtccctgatt cgagccaatc
    14941 ggcgcgccga gcggcatcac cgctgcacag ccgacttctt ccagacgttt acacaatacc
    15001 ggatcggccc cgcagtaagg cagcacgaca aatccctgtt gtaccagcgt ttcggcggct
    15061 ttcagggttt cgatgggatc gggcaacagc cagcgggcgt cagggtgaat ctccaatttt
    15121 agccagtttg tgcctaacgc ttcacgtgcc agatgggcgg cgaaaatggc ttcttccgct
    15181 gttttcgccc cggaagtatt cggtagcagg gccacacccg ccgcgataag cggttcgagg
    15241 atagcgtcgt tgtgctggcg caagtcgaca cgtttcatcg ccagtgtcac cagctggctg
    15301 ccggaagcgc ggatcgcctc caccatcagt tgtgaagaag cgaatttccc tgtgccggta
    15361 aacagatgtg aatcaaacgt tttgtccgca atacgtaaca tctcaacccc ctgcaataac
    15421 ctgaaaaagc aggatctggt cgccatcctg cacgatatgt tgcgtccact gctcacgcgg
    15481 gacgatttga tgattaatcg ccagagccgc gcccgcttgt cgttggtcga gttgctccag
    15541 tagttcgtga acagtttgcc cggcggcgca ctgcatcggt tgatcgttaa acaggatctg
    15601 cattgcttcc tccgcatacc gggcaaccac tggcgcggcg caacgccagg ctgcgccact
    15661 ggctcgactt accgtcgaac agtcggagtt ctcccgcagg cgtctctata ccgcttaata
    15721 acttaatggc ttccagtgcc tgcaaagtgc ccataacccc gaccaccggg ccaaccacgc
    15781 ccgccgtgcg gcagttgcgt tctggctcct ggttatctgg ccacaggcag cggtaacacc
    15841 cctgctccca gggcggcgtc agtaccatca actgaccgcc aaatccgacc gcgctggcgg
    15901 tgataagcgg cgtgttgagt gccacgcagg cggcattaat ctcctggcga gtcgccatat
    15961 tgtcggtaca gtcgagcacc acatcggctt gtgcaaccgc atcttttaac gcctcacccg
    16021 ttaaccgttg ttgtaatgct gtcagttgaa tatcgggatt caactgtgtc agtcgctgat
    16081 ggctgacctg cgatttcggg cgatcgatat cttcagtggt aaagaggatt tgtcgttgca
    16141 gattgcttaa atgcacatcg tcgtcatctg ccagtaccag cgtcccgacg ccagcgcccg
    16201 ccaggtatag cgcagcaggt gtacccagcc cgccaagacc gataatcagc acctggctgt
    16261 cgagcagttt ttgctgcccg tcaagagcga tatcgtcgag caggatttgg cggctataac
    16321 gcataaaatc acggtcattc atcgccaact cctgcaattt ccagcaactg tgccgttgcc
    16381 aaacgccagt ctgcggcttg agtaatggcg ctgacgacgg cgatactgcc gacacccgtt
    16441 gctatcaccg caggcgcacg cgccagactg ataccgccaa tcgccacggt gggataatcc
    16501 gccaggcgct caacatgccg tgccagctgt tccagcccct gcggtgcaga aggcatctgt
    16561 ttggtttgcg tcgggaacac atgtcccagc gcgatataag aggggcgtgc tgccagcgcg
    16621 acgtcgattt ccatatcgtc atgtgtcgaa acgcccagcc gcaggcctgc cgcgcggatg
    16681 gcattgagat cggtggcttg caaatcttcc tgccccaaat ggacgccata cgcctgatgc
    16741 ttgatcgcca gccgccagta atcgttgata aacaatcgcg cgttatagcg gcggcccagc
    16801 gcaattgccg ccacgacatc ggcttccacc tcttcatcgc gccgatcttt gatgcgtagc
    16861 tggagagtac gtacgcctgc atccaacaga cgttcgatcc actgtacgct gtccaccacc
    16921 gggtacagtc ctaaacgaaa aggtacagga ggaaaatcag gctgatacat cacgcttcct
    16981 ccttacgcag gtagatttct ccgcctctgg cacggaagtt ctccgacata tccgccattc
    17041 ccatttcaat agtttgcgtg gcggcgtaat cacgcacttc ctggctgatt ttcatcgagc
    17101 aaaatttcgg tccacacatg gagcaaaaat gggcgacttt gccggactct tggggcaggg
    17161 tttcatcgtg ataagcgcgg gcggtaaacg ggtcgagggc cagattaaac tggtcttccc
    17221 agcgaaattc gaagcgggct ttcgacatgg cgttatcgcg aatttgcgcg cccggatgcc
    17281 cttttgccag gtcagcggcg tgggcggcaa tcttataggt gataagcccc tgcttaacat
    17341 cttctttatt aggcagaccc agatgctctt ttggcgttac gtaacagagc atcgcgcagc
    17401 caaaccagcc aatcatcgcc gcaccaatcc ccgacgtgaa gtggtcatag cccggcgcaa
    17461 tatcggtagt tagcggcccc agagtgtaaa acggcgcttc gtggcagtgc tctaactcct
    17521 cggtcatatt gcggcggatc atctgcatcg gcacgtggcc tgggccttca atcatcacct
    17581 gcacatcata ttcccaggcg atttttgtca gttcgcccag cgtatgcagc tcggcaaact
    17641 gcgcttcatc gttggcgtcc tgaatagaac cggggcgcag accgtcgccc agcgacagcg
    17701 aaacgtcata agcggcacag atttcacaaa tttcgcggaa gtgttgatag aggaaatttt
    17761 cctgatgatg ggagaggcac catttcgcca taatcgaacc gccgcgcgag acgataccgg
    17821 tcaggcgttt cgcggtcatc ggcacatagc gcagcagcac ccccgcatgg atagtgaagt
    17881 aatccacacc ttgctcggcc tgttccagca gcgtgtcgcg gaacgcttcc caggtaagat
    17941 cttcggcgat cccgttaacc ttctccagcg cctggtagat cggcactgta ccgatcggca
    18001 ctgggctgtt acgcaaaatc cactcgcggg tttcgtgaat atagcgaccg gtggagagat
    18061 ccatcaccgt atccgctccc cagcgcgtgg accataccag cttttccact tcttcttcga
    18121 tggaagaggt gaccgctgag ttgccgatat tggcgttaac ttttaccagg aagttacggc
    18181 caataatcat cggctccgat tccggatgat taatgttggc cgggataatc gcacgtccgg
    18241 cagcaacttc atcacggaca aattccgcag tgatattttc cggcagacgt gcgccaaagc
    18301 tcattcccgg atgctggtgg cgtaaaactt cgctacggat gcgctcgcgg cccatattct
    18361 cgcggatggc gatgaattcc atttctggtg tgatgatgcc ctggcgggcg tagtgcagtt
    18421 gggtgacgcg gcgtcctgtt ttggcgcgtt ttggcgtgaa taggccgcta aaacgtagct
    18481 cgtcgaggcc atcatctgcc agtcgcgctt tagtgtaatc ggaactgcgc acggtaagtt
    18541 cttcggtatc gccgcgcgca tcgatccacg gctggcgtag ttttgccagc ccttgctgca
    18601 cgttaatggc gatctgcgga tcgccatacg ggccggaggt gtcgtagacc ggaatcgctt
    18661 cgttttcttc gtactgcggc tgttctttgc taccgccaat tagcgtcggg ctaagctgga
    18721 tctcacgcat cggcacgcgc acgccgggtt gtgtgccagt gatataaatg cgttttgagt
    18781 tgggaaaggc ggtgccttcc agggtgtcga taaaatgttg ggcccgggcg cgttgttcgc
    18841 ggcgggtcag ttttgttgca gacatagctc attccaaaaa gttaaggacg tggcttgtca
    18901 gacgacggat gaagcaagag acgatcgccg caaaggcgat gcaatagcag attaactctt
    18961 gttcccttcg caggtattag cctgatcagg ttccgcggat cccgaataaa cggtctcagc
    19021 cagttaaggc actccgacaa gaaattagcc cccacaaagg gggcgttgaa tgtaaattac
    19081 gcgttaacag cgcagaactc aagcaggatg tttgacgcgg gcagcgtcaa gcaccagcag
    19141 aatcagctta tcttccagca caaagcgcgc ttccagcgcc tcgccgatgt cagataaaac
    19201 ctgttgaaac tcaaggtaat tatcatgatc gatggcagtt tccaggctgg aatcgtagta
    19261 atccataatc tgttgggtat tggcttcgag ttgcggccaa atcttcgcgg cgcgcgcgag
    19321 ttgcccgttg ccttccagct tatgaagaat gcgctcataa atactgaaat gcccggcaga
    19381 caagtaatcg accaggctct gacaaaaatc atcaagggct ttttcgttta gcctcatgta
    19441 cgattctttg ccaggcttaa tgccaaccag attgtagtaa gccacgagca gatgcttacg
    19501 tacatgtagc cagcgatcaa ccagtttgtt acttcctctg acgcgctccg tcaggttatc
    19561 gagctggtta agcatgattg actccgcaag tttgtattca aaaactgctc agtgagaaat
    19621 gtaaaaacca tgttaaacat gccagtgatg caaaggtagt gcaagagcta tggatcgtat
    19681 aattgaaaaa ttagatcacg gctggtgggt cgtcagccat gaacaaaaat tatggttgcc
    19741 gaagggagaa ttgccatatg gcgaagcggc aaatttcgat cttgtgggtc agcgcgcact
    19801 acaaatcggc gaatggcagg gggaacctgt ttggttagta caacagcagc ggcgtcacga
    19861 tatggggtcg gtacgtcagg tcattgatct cgatgttggg ctgtttcaac tggccggacg
    19921 aggcgtacaa ctggcggagt tttaccgatc gcataaatac tgtggttact gcgggcatga
    19981 aatgtatcca agcaaaaccg aatgggcgat gctgtgcagc cattgccgtg agcgttacta
    20041 cccgcaaatc gccccctgca ttattgttgc catccgtcgc gatgattcga tcctcctcgc
    20101 ccagcatacc cgccatcgta acggtgtcca tacggtactt gccggattcg tcgaagtggg
    20161 cgaaaccctc gagcaggcgg tcgcgcggga agtgatggaa gagagcggaa ttaaagttaa
    20221 aaacttgcgt tatgtgactt cccagccgtg gccgtttcct cagtctttaa tgaccgcgtt
    20281 tatggcggaa tatgacagcg gcgacatcgt gatcgacccg aaagaattgt tcgaggcgaa
    20341 ctggtatcgc tatgacgatt tgccgttact cccgccgccc ggcaccgtag cgcgccgtct
    20401 gatagaagat acggtggcga tgtgtcgggc agagtatgag tgatgataca ctgactgcct
    20461 gacgcactaa ggaacagcca aaatgaccga acttaaaaac gatcgttatc tgcgggcgct
    20521 gctgcgccag cccgttgatg tcactccagt atggatgatg cgccaggcgg gtcgctatct
    20581 accggaatat aaagccacgc gtgcccaggc gggcgatttt atgtcgctgt gcaaaaacgc
    20641 cgagctggcg tgcgaagtga ctttgcaacc gctgcgtcgc tacccgctgg atgcggcgat
    20701 cctcttttcc gatatcctca ccgtgccgga cgcgatgggg ttagggctct attttgaggc
    20761 cggagaaggt ccgcgtttta cctcaccagt cacctgcaaa gctgacgtcg ataaactgcc
    20821 aattccggac ccggaagatg agctgggtta cgtgatgaac gcggtgcgta ccattcgtcg
    20881 cgaactgaaa ggcgaagtgc cgctgattgg tttttccggc agcccgtgga cgctggcgac
    20941 ctacatggtg gaaggcggca gcagcaaagc gttcaccgtg atcaaaaaaa tgatgtatgc
    21001 cgatccgcag gcgctgcatg ctctgctcga taaactggcg aaaagcgtca ctttgtatct
    21061 gaatgcgcag attaaagccg gtgctcaggc tgtgatgatt ttcgacacct ggggcggcgt
    21121 gcttaccggg cgcgattatc aacagttctc gctttattac atgcataaaa ttgttgatgg
    21181 tttactgcgt gaaaacgacg gtcgccgcgt accggtcacg ctgtttacca aaggcggcgg
    21241 acagtggctg gaagccatgg cagaaaccgg ttgcgatgcg ctgggcctcg actggacaac
    21301 ggacatcgcc gatgcgcgcc gccgtgtggg caataaagtc gcgttgcagg gtaatatgga
    21361 tccgtcgatg ctgtacgcgc cgcctgcccg cattgaagaa gaagtggcga caatccttgc
    21421 cggtttcggt cacggtgaag gccacgtctt taacctcggc cacggtatcc atcaggatgt
    21481 gccgccagaa catgctggcg tgtttgtgga ggcagtgcat cgcctgtctg cgccttatca
    21541 tcagtaagga gtggttatgg atctcgcgtc attacgcgct caacaaatcg aactggcttc
    21601 ttctgtgatc cgcgaggatc gactcgataa agatccaccg gatctgatcg ccggagccga
    21661 tgtcgggttt gagcagggcg gagaagtgac gcgagcggcg atggtgctgc tgaaatatcc
    21721 cactcttgag ctggtggagt ataaagtggc acgcatcgcc accaccatgc cttacattcc
    21781 tggttttctc tccttccgcg aatatcctgc gctgctggcc gcgtgggaga tgctgtcgca
    21841 aaaacctgat ttagtgtttg tcgatggtca cgggatctcg catcctcgcc gtcttggcgt
    21901 cgccagccat tttggtttgc tggtggatgt gccaacgata ggcgtggcga aaaaacgact
    21961 atgcggtaaa ttcgaaccgc tctccagcga accgggcgcg ctggccccac tgatggataa
    22021 aggcgagcag ctggcctggg tctggcgcag caaagcgcgc tgtaacccgt tgtttatcgc
    22081 taccggccat cgggtcagcg tggacagcgc gctggcgtgg gtacaacgct gcatgaaagg
    22141 ctatcgtctg ccggagccaa cgcgctgggc ggacgcggtg gcctcggaac gtccggcgtt
    22201 cgtgcgctat acagcaaatc agccctaatt caggtaaact gcgggtaatt tccgtatttg
    22261 agaactcaac atgttacaaa acccaattca tctgcgtctg gagcgcctgg aaagctggca
    22321 gcatgtcact ttcatggctt gcttatgcga acgcatgtac cccaattacg ccatgttttg
    22381 ccagcaaacc ggttttggcg atgggcaaat ttaccgccgt attctcgatc tcatctggga
    22441 aacgctgacc gtcaaagacg caaaagtaaa tttcgacagc caactggaga aatttgaaga
    22501 agcgattcct tcagccgacg atttcgatct gtacggcgtt tatccggcaa tcgatgcatg
    22561 cgtggcgtta agtgaactgg tccattcgcg tttgagtggt gaaacgctcg aacacgcggt
    22621 ggaagtgagt aagacctcca tcacgaccgt tgcgatgctg gaaatgactc aggctggtcg
    22681 cgaaatgagc gatgaagagc tcaaagaaaa cccagctgta gagcaagaat gggatattca
    22741 gtgggaaata ttccgacttt tagctgagtg cgaagaacgc gatatcgagc tgataaaagg
    22801 ccttagggca gacctgcgtg aggcgggtga gagcaatatt ggtataattt ttcagcaata
    22861 agaccagaaa acgtgattta acgcctgatt tgtcgtacct ggagtcttcc ctttcgcccc
    22921 ccgtctggtc tacatttggg gggcgaaaaa aagtggctat cggtgcgtgt atgcaggaga
    22981 gtgctattct ggcatttccg tcgcactcga tgcttagcaa gcgataaaca cattgtaagg
    23041 ataacttatg aacaagactc aactgattga tgtaattgca gagaaagcag aactgtccaa
    23101 aacccaggct aaagctgctc tggagtccac tctggctgca attactgagt ctctgaaaga
    23161 aggcgatgct gtacaactgg ttggtttcgg taccttcaaa gtgaaccacc gcgctgagcg
    23221 tactggccgc aacccgcaga ccggtaaaga aatcaaaatt gccgcagcta acgtaccggc
    23281 atttgtttct ggcaaggcac tgaaagacgc agttaagtaa gattgcgtgg cagtgaacag
    23341 ttttaacgaa ggggtggttt cacccctttt gtctttctgg cgtcgatcat tgatgctggc
    23401 tggcgctctg tttctcactg cctgtagcca caactcttca cttcctccct ttaccgccag
    23461 tggatttgct gaagaccagg gcgcggtacg tatctggcga aaagacagcg gcgataatgt
    23521 gcatctgctt gccgtgttta gcccgtggcg cagtggcgat accacgacgc gagagtatcg
    23581 ctggcagggc gataacctca cgctcatcaa tatcaatgtt tacagcaaac cgccggtgaa
    23641 cattcgcgcg cgttttgacg atcgcggtga tctgagcttt atgcaacgtg aatccgatgg
    23701 ggaaaagcag cagctttcta acgaccaaat cgatttatac cgttatcgtg ctgatcagat
    23761 ccgccagatt agcgatgcct tacgtcaggg gagagtcgtg ctgcgccagg ggcgctggca
    23821 tgcgatggaa cagaccgtga ccacctgcga agggcaaacc attaaacctg atttagattc
    23881 gcaggcgata gcgcatatcg agcgccgcca gagccgctct tctgttgatg tcagcgtggc
    23941 atggctggaa gcgcccgaag gttcgcaatt actgttagtg gcaaactctg atttctgtcg
    24001 ctggcaaccc aacgagaaaa cgttctgatt taccagtggc ccatacccat atgaccgcca
    24061 ccaccgcagc cgccgtagcc catgcccatt ccggctccgc gcggaatacc cgcttcagcc
    24121 atcgcgatat ctcgtttcac ccgtaactca tctaacgact gacgcaaatt ctccatctct
    24181 ttggcgaccg cgttaatttt gctgctatcc ggtgggttcg cggctaacag ggcattgtat
    24241 tcataacgct tcgtcaccag ttgctgttgc agtgcgctgc tttgagcgta aaagtcatta
    24301 tggattttct gccacgctgt ctgttgttcg ctggtcaaag gcgcggcatt ttgctgccac
    24361 ataccgtgtc cgccgtgagc aaatgcagat gtcgatccca tcgccattgc tgaaagcgcc
    24421 atcattacca gggcaatttt cgtgttccgt ttcatggtta atcctccagt ggttgtctta
    24481 cttcgggtat tgcatcttcc gtgccaacga tgaaacgctg atatgacggg taatctggca
    24541 tgataaacga gtaaaaatga ctcgcctgct gcgggtagcg agtcattttt actcattgaa
    24601 acttaagcct ttgtgttaca gcgcagggta agcgctgata aaagatggca tgatttctgc
    24661 tgtcagaaag ggatgagcag gcaaagaaga agatgcgttt tatgcaacgt tctaaagact
    24721 ccttagctaa atggttaagc gcgatcctcc ccgtggtcat tgttgggctg gtagggttgt
    24781 ttgcggtgac ggtgattcgc gattatgggc gcgagactgc cgccgccaga caaacgctgc
    24841 tggaaaaagg cagtgtactt attcgcgctc ttgaatccgg ctcgcgcgtc ggcatgggga
    24901 tgcgcatgca tcatgcgcag cagcaggcct tactggaaga aatggccggg cagcctggag
    24961 tacgttggtt tgcggtcacg gatgaacaag gaacaatcgt gatgcatagc aactccggca
    25021 tggtgggaaa acagctttat tccccgcagg aaatgcagca gttacatcct ggagatgaag
    25081 aagcgtggcg gcggatcgat agcgcagacg gcgagcctgt tctggaaatt tatcgccagt
    25141 ttcaaccgat gtttgctgct ggaatgcacc ggatgcgcca tatgcagcag tatgccgcaa
    25201 caccacaagc aattttcatc gctttcgacg ccagtaatat tgtgagtgcc gaagatcgtg
    25261 agcagagaaa caccctgatt atcctcttcg ccctggcgac ggtcttgctg gcaagcgtat
    25321 tgtcattctt ctggtatcgc cgctatctgc gctcgcgcca gcttctacaa gatgaaatga
    25381 agcgcaaaga gaagctggtg gcgctggggc atcttgcggc aggcgttgcc cacgaaatcc
    25441 gtaacccact ttcctcgatt aaaggactgg cgaaatactt tgccgagcgc gcgcctgcag
    25501 ggggagaagc gcatcaactg gcgcaggtga tggcgaaaga ggccgaccgt ttaaaccgcg
    25561 tggtaagcga gttgctggaa ctggttaagc caacgcatct ggctttgcag gcggtggatc
    25621 tcaacacgct gattaaccac tcattacagc tggtaagcca ggatgcaaac agccgggaga
    25681 tccagttacg ctttaccgcc aacgacacat tactggaaat tcaggccgat ccggacaggc
    25741 tgactcaggt cctgttgaat ctctatctca atgctattca ggcgattggt cagcatggcg
    25801 tgattagcgt gacggccagc gaaagcggcg cgggcgtgaa aatcagcgtt accgacagcg
    25861 gtaagggaat tgcggcagat cagcatgaag ctatcttcac tccgtacttc accaccaaag
    25921 ccgaaggcac cggattgggg ctgacggtcg tgcataatat tgttgaacaa cacggtggta
    25981 caattcaggt cgcaagccag gagggaaaag gctcaacgtt caccctctgg cttccggtca
    26041 atattacgcg taaggaccca caaggatgac gcacgataat atcgatattc tggtggtgga
    26101 tgatgacatt agccactgca ctattttgca ggctttactg cgcggctggg gctataacgt
    26161 cgcgctggcg aacagcgggc gacaggcgct ggagcaggtg cgggaacagg tttttgatct
    26221 tgtgctttgc gatgtgcgaa tggcggagat ggacggcatc gccacgctga aagagatcaa
    26281 agcgttaaat ccggcgattc cggtactgat tatgaccgcg tactccagcg tcgagacggc
    26341 ggtagaagcg ttgaaaaccg gggcgctgga ttatctcatc aagccgctgg atttcgataa
    26401 cctacaggcg acgctggaaa aggcgctcgc gcatacgcac agtattgatg ctgaaacacc
    26461 tgcggtgact gccagccagt tcggtatggt cggtaaaagc ccggcgatgc aacacctgct
    26521 cagtgaaatc gccctcgtcg cgccatcgga agccacggtg ctgatccacg gcgattccgg
    26581 caccggtaaa gagctggtcg ccagggcgat tcacgccagt agcgcacgta gcgaaaaacc
    26641 gctggtaacg ctcaactgtg cggcactcaa cgaatccttg ctggaatctg aattgttcgg
    26701 tcacgaaaaa ggggcgttta ctggagccga taaacgccgg gaagggcgct ttgttgaggc
    26761 ggacggcggc acgctgtttc tcgatgaaat tggcgatatc tcgccgatga tgcaggtgcg
    26821 tctgctgcgt gcgattcagg agcgcgaagt tcagcgtgtc ggtagcaacc agactatctc
    26881 ggttgatgtc cggctgattg cggcgaccca tcgcgatctt gccgcagagg tgaatgccgg
    26941 gcgttttcgc caggatctct actatcgcct gaatgtggtg gcgattgaag taccatcgct
    27001 gcggcaacgg cgggaagata tccctctgct ggctggccat tttctgcagc gctttgccga
    27061 gcgtaatcgc aaggcggtaa aaggttttac gccccaggcg atggatctgt tgattcatta
    27121 cgactggccg ggaaatattc gtgagctgga aaacgcggtg gaacgggcag tggtgctgct
    27181 gaccggggaa tatatttccg aacgcgagct gccgctggcg attgccggta cgccgatccc
    27241 gctggggcaa agtcaggata ttcagccgct ggtggaagtg gaaaaagagg tgattctggc
    27301 ggcactggag aaaacgggcg gcaacaaaac tgaagccgcc cgtcagttag ggatcacgcg
    27361 taaaacgtta ttggcaaaac tgtcgcgtta gttctgctcg cgttcgatag cacgccagcc
    27421 gatatctttc cggcagaagc agtcgtccca atggatatcg gtcattaagg cataggcgcg
    27481 tttctgcgct tctgccacgg tatgacccag cgcggtgacg cacagtacgc gcccgccgtt
    27541 ggtcactacc tgctcgtcat ccgccagttt tgtgcccgcg tggaacactt tgccgcctgc
    27601 cacttcttcc agcggcaggc cgtggatcac gtcaccggtg cggtaatcac ccggatatcc
    27661 acccgcagcc atcaccacgc cgagagaagc gcgttcatcc cactcggacg ttttctcgtc
    27721 cagtttgctt tcacaggccg ccaggcagag ctcaaccaga tcggacttca tgcgcagcat
    27781 aatcggctgg gtttccggat cgccaaagcg gcagttaaat tcgataacct tcggattgcc
    27841 ctgtttgtcg atcatcaggc ccgcgtagag aaaaccggtg taggtgttgc cttccgccgc
    27901 catgcctttc acggttggcc agataatacg ttccatggtg cgctgatgaa cgtcatcggt
    27961 tactaccggc gcgggggagt aagcgcccat cccgccggtg tttggtccgg tatctttatc
    28021 gcctacgcgt ttgtgatcct ggctggtagc catcggcagc acatgctcgc cgtccaccat
    28081 cacgataaag ctcgcttctt cgccatcgag gaactcttcg ataacgatgc gatgacccgc
    28141 gtcgccaaaa gcgttgcccg ccagcatatc gtgaacagcc gcttccgctt cttccagcgt
    28201 catcgccacg ataacgcctt tcccggcagc cagaccgtca gctttaatga cgattggcgc
    28261 gcctttctca cgcagatacg ccagcgctgg ttctacctcg gtgaagttct ggtattccgc
    28321 cgtgggaatc ttatgacggg ccaggaaatc tttggtaaac gcttttgagc cttccagttg
    28381 cgccgcacct gcggttgggc cgaagatttt cagcccggca gcgcggaagg tatcgaccac
    28441 gcctttcacc agcggcgctt ccgggccgac gatggtcaga tcaatctttt cgttttgtgc
    28501 gaaatccaac agcgccggga tatcggtcac gccgatagca acgttttgca gcgcgggttc
    28561 cagcgcagtg cctgcattac ccggagcaac aaaaacagtt tcaaccagcg gcgactgcgc
    28621 cgctttccag gccaacgcgt gctcgcgccc gccgttacca atcactaata ctttcatcta
    28681 ttgctccatt aatggcggaa gtggcgcata tcggtgaaga gcatcgcaat accgtgttca
    28741 tcggcggcgg caatcacttc gtcatcacgg atagaaccgc caggctggat tacgcaggtc
    28801 acgcccgcag cggcggcggc atcaataccg tcgcggaacg ggaagaacgc gtcagaagcc
    28861 atcgaggaac ctttcacttc caggccttca tcggcagctt taataccggc gattttcgcg
    28921 gagtacacgc ggctcatctg gcccgcgcca ataccgatgg tcatattgtt tttggcatag
    28981 acgatagcat tggatttcac gaatttcgcc actttccagc agaacagcgc atcacgcagt
    29041 tcctgttcgg tcggctgacg ttgggtgacg acgcgcagtt cttccgcacc gaccatgccc
    29101 aggtcacgat cctgaaccag cagaccgccg ttcacgcgtt tgaaatcaag actcggaaca
    29161 cgctcgcccc actgaccgca ggtcagaacg cgtacgttct gcttggcggc ggtgattttc
    29221 agggcttctt cgctggcgga aggcgcaata atcacttcga caaactgacg agaaatgatg
    29281 gcctgtgcgg tttccgcatc cagctcgcgg ttaaaggcaa tgatgccgcc gaatgcggag
    29341 gttgggtcgg ttttgtacgc gcgatcgtaa gcatcaagaa tggaattgcc gatagccacg
    29401 ccgcaagggt tggcgtgctt cacaatcaca catgccggct cggcgaactc tttcacgcac
    29461 tccagcgccg catcggtatc ggcgatgttg ttataagaga gggctttacc ctgaacctgg
    29521 gttgcggtgg caacggaggc ttcttttaca ttctcttcta tatagaaggc agcctgctgg
    29581 tggctgttct cgccgtaacg catatcctgc ttcttaatga agttcaggtt cagcgtgcgt
    29641 gggaagcgac cggcggcttc tttgctttca ccgtggtaag ccggaaccat gctgccgaag
    29701 tagttggcaa tcatgctgtc gtaggcggca gtgtgttcga aggctttgat ggcgaggtcg
    29761 aaacgggttg caagcgtcag cgatccttcg ttgtcatcca tctctttaat aatgacatcg
    29821 tagtcgctgc tcttcaccac gatggcgaca tctttatggt tcttggcggc ggagcgcacc
    29881 atggtcgggc cgccgatatc gatgttctca accgcatctt ccagcgagca accttcacgg
    29941 gccacggtct gggcgaacgg atacaggtta acaaccacca tatcgatagg ctggatctga
    30001 tgttcttcca taatggcatc gtcctggccg cgacggccca gaatgccacc atgtactttc
    30061 ggatgcaggg tcttcacgcg tccatccatc atctccggga aaccggtgta atcggaaact
    30121 tcggttaccg gcagaccttt ttctgctaac agacgggcag tgccccctgt agacagcagc
    30181 tccacaccgc gtgcggaaag tgcctgggcg aattcgacga taccggcttt gtcagaaaca
    30241 ctgagcagag cgcggcggac tggacgacgt tgttgcatgg taaatcccct ggatttgact
    30301 attacagaga gcgttagctg aatttttcgc gaaaaactca gctaacgccc ctaacggggc
    30361 atccttattt ttcgcccgca ttgtaacgaa aacgtttgcg caacgctcgc gaatttttct
    30421 ctttcaatgg tgatcaccat tttgacagtg gttaccgtgg gaaaaataca gaaattacat
    30481 tgatgattgt ggataactct gtgcgtaaaa aggtataaag cggggttttg ctggggaatg
    30541 cagcagtcag tcatttttct gcaatttttc tattgcggcc tgcggagaac tccctataat
    30601 gcgcctccat cgacacggcg gatgtgaatc acttcacaca aacagccggt tcggttgaag
    30661 agaaaaatcc tgaaattcag ggttgactct gaaagaggaa agcgtaatat acgccacctc
    30721 gcg
//
LOCUS       DAPGOF010000047        30665 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.047, whole genome shotgun sequence.
ACCESSION   DAPGOF010000047 DAPGOF010000000
VERSION     DAPGOF010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30665
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.047"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            91..1674
                     /locus_tag="RHV13_001798"
     CDS             91..1674
                     /locus_tag="RHV13_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDV1241338.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            1749..2087
                     /locus_tag="RHV13_001799"
     CDS             1749..2087
                     /locus_tag="RHV13_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDV1241339.1"
                     /translation="MRKKLAFLDTSLDDLRAFPESSRQEIGYQLDRIQQGLNPYDWKS
                     FSTIGPGVREIRTRDADGIYRVMYVAKFEEAVYVLHCFQKKTQTTSQSDIDLAKRRYK
                     ELVQERKNEN"
     gene            2077..2367
                     /locus_tag="RHV13_001800"
     CDS             2077..2367
                     /locus_tag="RHV13_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDV1241340.1"
                     /translation="MKIETFDSVWDAVSDTPEQAENMRIRAELVTIINNWIEQQGFSQ
                     AQAASALGVTQPRISELARGKIQIFSIDKLITMMAHAGLHIQRIEIQYPHAA"
     gene            complement(2420..4273)
                     /gene="asmA"
                     /locus_tag="RHV13_001801"
     CDS             complement(2420..4273)
                     /gene="asmA"
                     /locus_tag="RHV13_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HDV1241341.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(4295..4876)
                     /gene="dcd"
                     /locus_tag="RHV13_001802"
     CDS             complement(4295..4876)
                     /gene="dcd"
                     /locus_tag="RHV13_001802"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HDV1241342.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(4968..5609)
                     /gene="udk"
                     /locus_tag="RHV13_001803"
     CDS             complement(4968..5609)
                     /gene="udk"
                     /locus_tag="RHV13_001803"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDV1241343.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5927..9244
                     /locus_tag="RHV13_001804"
     CDS             5927..9244
                     /locus_tag="RHV13_001804"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDV1241344.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGGLVVLLTAGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRMLLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNIESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMMAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQKFRRSFS
                     DPALSHALDRRVFHEFFQLAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAESVQKLRLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(9283..10140)
                     /gene="alkA"
                     /locus_tag="RHV13_001805"
     CDS             complement(9283..10140)
                     /gene="alkA"
                     /locus_tag="RHV13_001805"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HDV1241345.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVAEDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDEL"
     gene            10274..11626
                     /gene="yegD"
                     /locus_tag="RHV13_001806"
     CDS             10274..11626
                     /gene="yegD"
                     /locus_tag="RHV13_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDV1241346.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEESKIALSSTAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(11638..13578)
                     /gene="yegI"
                     /locus_tag="RHV13_001807"
     CDS             complement(11638..13578)
                     /gene="yegI"
                     /locus_tag="RHV13_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HDV1241347.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTKNHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQQLKKCTVSAMHVYPGHLADCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAITPADRQAVLTKMAAKRHRLESTLTVGATELQRFRLQAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(13575..14336)
                     /gene="pphC"
                     /locus_tag="RHV13_001808"
     CDS             complement(13575..14336)
                     /gene="pphC"
                     /locus_tag="RHV13_001808"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HDV1241348.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDILATDIVLTIRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(14333..14992)
                     /locus_tag="RHV13_001809"
     CDS             complement(14333..14992)
                     /locus_tag="RHV13_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDV1241349.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMNGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(15213..15272)
                     /gene="ibsA"
                     /locus_tag="RHV13_001810"
     CDS             complement(15213..15272)
                     /gene="ibsA"
                     /locus_tag="RHV13_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HDV1241350.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(15545..15601)
                     /locus_tag="RHV13_001811"
     CDS             complement(15545..15601)
                     /locus_tag="RHV13_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HDV1241351.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(15873..15929)
                     /locus_tag="RHV13_001812"
     CDS             complement(15873..15929)
                     /locus_tag="RHV13_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HDV1241352.1"
                     /translation="MMKLLIIVVLLVISYPAY"
     gene            16208..17455
                     /gene="mdtA"
                     /locus_tag="RHV13_001813"
     CDS             16208..17455
                     /gene="mdtA"
                     /locus_tag="RHV13_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HDV1241353.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLIGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNVV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLSSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            17455..20577
                     /gene="mdtB"
                     /locus_tag="RHV13_001814"
     CDS             17455..20577
                     /gene="mdtB"
                     /locus_tag="RHV13_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HDV1241354.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEELQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVTAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            20578..23655
                     /gene="mdtC"
                     /locus_tag="RHV13_001815"
     CDS             20578..23655
                     /gene="mdtC"
                     /locus_tag="RHV13_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HDV1241355.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLSVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQTALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLNWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            23656..25071
                     /locus_tag="RHV13_001816"
     CDS             23656..25071
                     /locus_tag="RHV13_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1241356.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLLLMPNYTMQTRRFDLSGFLLLAVGMAVLTLVLDGSK
                     GTGLSPLTIAGLVAVGVVALVLYLLHARNNHRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMALIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            25068..26471
                     /gene="baeS"
                     /locus_tag="RHV13_001817"
     CDS             25068..26471
                     /gene="baeS"
                     /locus_tag="RHV13_001817"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HDV1241357.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLQISAGQRDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            26468..27190
                     /gene="baeR"
                     /locus_tag="RHV13_001818"
     CDS             26468..27190
                     /gene="baeR"
                     /locus_tag="RHV13_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HDV1241358.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAARYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            27381..27713
                     /locus_tag="RHV13_001819"
     CDS             27381..27713
                     /locus_tag="RHV13_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HDV1241359.1"
                     /translation="MAGWFELSESSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            27922..28218
                     /locus_tag="RHV13_001820"
     CDS             27922..28218
                     /locus_tag="RHV13_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HDV1241360.1"
                     /translation="MTYTDVRFLLTGGRKMARTMTVDLGDELREFIESLIESGDYRTQ
                     SEVIRESLRLLREKQAESRLQALRDMLAEGLSSGEAQPWEKDAFLRKVKAGIRK"
     gene            28220..28516
                     /locus_tag="RHV13_001821"
     CDS             28220..28516
                     /locus_tag="RHV13_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDV1241361.1"
                     /translation="MRIIKLMPKANEDLEGIWYYSYHHFGEPQADRYVEHLSDVLQIL
                     SNNNIGTPRPELGEGIFVLPFERHVIYFLQSPGEIIVIRILNQNQDATRHLHWS"
     gene            28619..29980
                     /gene="yegQ"
                     /locus_tag="RHV13_001822"
     CDS             28619..29980
                     /gene="yegQ"
                     /locus_tag="RHV13_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HDV1241362.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPIAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(30310..30627)
                     /locus_tag="RHV13_001823"
     CDS             complement(30310..30627)
                     /locus_tag="RHV13_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1241363.1"
                     /translation="MKKIVAISLISIFIVSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
BASE COUNT         7154 a         8125 c         8041 g         7345 t
ORIGIN      
        1 aaatagtgct gcaacattgc atttttgccc cgatttatcc atgatcgaat tgtgacattt
       61 gtcatacaac gaataggttt tgtacttact atggaatgga ttgccgatcc gtctatctgg
      121 gccgggttaa tcacgctgat tgtgatcgaa ctggtcctcg gcattgataa cctggtcttt
      181 attgccatcc tcgccgaaaa actaccgccg aagcagcgcg accgcgcacg ggttaccggg
      241 ctgctgctgg cgatgttaat gcgcctgtta ctgctggcgt caatctcctg gctggtcacc
      301 ctgacgcaac cgctgttcag cttccgctcg tttaccttta gcgcccgcga cttaatcatg
      361 ctgtttggtg gtttcttcct gttgttcaaa gccacgatgg agctgaacga acggctggaa
      421 gggaaagaca gcaataatcc cacacaacgc aaaggtgcga agttctgggg ggtggtgaca
      481 caaattgtgg tactggacgc catcttctca ctcgactcag tgattaccgc cgtcgggatg
      541 gtcgaccatt tactggtcat gatggccgcc gtggttatcg ctatcagcct gatgttgatg
      601 gccagcaagc cgttaacgca atttgttaac agtcacccga ccatcgttat tctctgctta
      661 agcttcctgc tgatgattgg ctttagcctg gtggcagaag gtttcggctt cgtcattccg
      721 aaaggctacc tgtacgctgc cattggtttc tcggtgatga tcgaggcgct caatcagttg
      781 gctatcttta accggcgacg ttttctttcc gctaaccaga cgctacgcca gcggactacc
      841 gaagcggtaa tgcgcctgct tagcgggcaa aaagaagatg cggaactgga cgccgaaacc
      901 gcgtccatgc tggtggatca tggtaaccag cagatcttta atccgcagga acggcggatg
      961 attgagcggg tacttaatct taaccagcgt actgtcagca gcattatgac gtcgcgccac
     1021 gatattgagc atatcgatct caacgcgccg gaagacgaga tccgccagtt gctggagcga
     1081 aatcagcata cgcggctggt ggttaccgac ggtgatgacg cagaagattt gctcggtgtt
     1141 gttcacgtta tcgacctgct acaacagtca ctgcgtggcg aaccgctcaa cctgcgggtg
     1201 ttgattcgcc agccgctggt gttcccggaa accttgccgt tgttacctgc cctggagcag
     1261 ttccgtaatg cccgcacgca ctttgctttt gtggtggatg agtttggctc ggtggaaggg
     1321 attgtgacat taagtgacgt cactgaaacc attgccggta acttaccgaa cgaagtggaa
     1381 gagatcgacg cccgccatga tattcagaag aatgcggacg gttcctggac ggcgaatggt
     1441 catatgccgc tggaggatct ggtgcaatat gtgccgctgc cgctggatga aaaacgtgaa
     1501 tatcacacca ttgccgggct gttgatggaa tatttacagc gtattccaaa acctggcgaa
     1561 gaagttcagg tgggggatta tctgcttaaa acgttgcagg tggaaagcca tcgcgtgcag
     1621 aaggtgcaga ttattccgct acgtaaggat ggcgagatgg agtacgaggt gtgataaaac
     1681 atcaaatgcg caggttcaaa ctgcgcattt gatttataca aaatttagta taatctaagg
     1741 atggagtaat gagaaaaaag ctggcttttc ttgataccag tcttgacgat ttacgcgcat
     1801 ttccagaatc gtctcgacag gaaattggct atcaactcga caggatccaa caagggttaa
     1861 acccatacga ctggaaatct ttttcaacta tcggccctgg tgtgcgagag attcgtactc
     1921 gcgatgctga tgggatctat cgcgtcatgt atgtcgcaaa atttgaagaa gcggtttacg
     1981 tattgcattg ttttcaaaag aaaacacaga ctaccagtca atcggatatt gatttagcca
     2041 aacgacgcta taaagaactt gttcaggagc ggaaaaatga aaattgaaac ctttgatagt
     2101 gtgtgggatg cggtgagtga cacccctgaa caagcggaaa atatgcggat ccgtgccgag
     2161 cttgtaacga tcattaataa ctggattgaa caacaagggt ttagccaggc tcaggcagct
     2221 tctgctttag gggtcactca acctcgcata tctgaacttg ccagaggtaa gatccagatc
     2281 tttagtatcg ataaattgat caccatgatg gctcatgcag gactacatat tcaacgtatt
     2341 gaaattcaat atccacatgc tgcttagtgc tataaataca gatggcgaca aattgacgcc
     2401 gccatctgca taacttgtcc tacatcttct ccagcaactt cttcacatct ttgccattgc
     2461 gggaatcttt attccgctcg gcccagtcat tcaggcgacg tttcgcttcg tcctgtagat
     2521 gtttgcgcag taactgatcc acttgcagac tgtaattgag ttgctgccag ttgccataaa
     2581 cccgcagcgg tactggcgtt tctttgagga aatcaatcag tttgctttcc ccgttccagc
     2641 cacccacgac ccgaatatca aactgggtgt cgcaggtttg atctgccaga ttcaacatgc
     2701 cttcccctgt cagcgccagc acaggcgatt gaccttgcat gtcgtttaac gtcacgacgc
     2761 catccttcaa cgtcaaatcg gtggtaaagc ggtcaagacg cgttacgtta tcgaagtttt
     2821 cagcggcctt cacatcacca ccattacgtt ctaccgcttg ctgaatcatc tgctggaagt
     2881 tcatcccttc catgcgcgtg tcggtcattt cgacatgtgc ctgtccttgc cagttgtggc
     2941 ggaatgcgtc ggcatctatg tcagcaccgg agaaatcacc agccagtgac atttttccgg
     3001 tcaacgaaat cggatagtta aacgccttca gaatggtacc aatctcaacg ttttccagcc
     3061 gtggctggaa gtttatccgc ggatttattg atgtcgcgtc cagcgtgccc ggcagtgaaa
     3121 cctgtccacc gttaagcttg ccctgcagtt gagtaatttc cagcaaaccc gacttgttgg
     3181 tcatttgcgt ggcaacatct gtaaaattca ttccgcgcca gcgcacgtta ctggcctgca
     3241 acaaaatatc agccgtaaag ccttgcagtc cctgatatgc cggttcatca atacgcgaag
     3301 aaatgaccgg gcgcggcaat gttgattggc tctgcccctg ctgcgcggca ccgttttcac
     3361 cattcgctgt ttcattaagc gggatgaggt tgtcaagatt cagttgcggg aattgcagtc
     3421 tcagctgcca ttcaggtttc tctgtcagcg tgacctgtgc ttgcccgctc agcgtgctgt
     3481 cattggcggt caagctgatt tggttaaatg aaaggcgttt atgtgactcc tgccactggg
     3541 cctggaaact cccctgcccc tgaatacctt gttttggtaa atctgcaccc tgcaactgcc
     3601 agttaatttg ttcaatagcc gccgttaaat catgcggata atcagaagca tctaccgtac
     3661 cgttaaggga tatcgtgaga tcgcgctgat cgcgattaac ccgcccggag aactcaaatg
     3721 aaccacgatg ttggggatct tgttccattt gcaggcgaat attgcggatt gtcacctgct
     3781 cgtcatcttc atgctggaac accagcacgc tgtccgccac cttaagactg gatatatcaa
     3841 acgaccatcc gcgatcgtct gacagatccg gcaaggtatt gtcgcgcggt gcaaccggag
     3901 cgtcttcact gcgcaccgct tccgtctgcg gcgtcagttg aatcactgcc ccttttagca
     3961 tcacctgctt aacgctcagt tgatgactca gtagtggtaa aagcgccacg tccagacgca
     4021 tgttgtcggc gcgaaccagt ggctggcttg cgccctgggc ggtgagagac attcgcccgg
     4081 agaggatact aagctgcggc cagacgtgcc aacgcagtgg cccgtcgagc tgcaattgat
     4141 aaccgctacg tgcagcaact tgcttgacca tatagtcgcg gaaatcattc ggattcacca
     4201 gcaacactaa cgcagataac ccggccacca gcacgaccag gagtatcatc agcgtcgtca
     4261 gaaatcgtct catggtatcc tcaatgggcc tgaattagtc tttatcgatt cggctggcta
     4321 ccgcgccctg ctggttgcga tatttcgcat cttcacggcg gttgtaaggt cgcgccgccg
     4381 ggccggaaag cggctcaaag ctcagcgcac caattaacat gcccggacgc agcgccagcg
     4441 gcagcttacc ggagttgtag aactccagca caatgcaacc agaccagccc ggatcgatac
     4501 ggtgcgcggt gacgtgcacc atcagcccca gacgcgccaa agaggaacgc ccgtccagcc
     4561 agcccaccag atcggctggc agcgtcaccg actccagcgt caccgccagc gccagctctc
     4621 ctgggtgaag atagaacgcc tcgccctcgt cgagaacgat ctcatcgctc atcacgcggt
     4681 caagcgcggc gctcacttca tctttgggac cgctcagatc gataaacgct gccgtgtgac
     4741 cacggaaggt acgaaattta ttgcccaggc gtacatccac cgtcgcgccg ttaatacgct
     4801 ccactggcgg acgtgggttg atcgacaaac ggccttcatc aagccaggct tcaatatctc
     4861 ggtcacacag acgcatggca ttttctcctt tcgcgcatca ctcccttaac gccaattacg
     4921 tcaagggcat accaggttat cactgaacgg tacacaattt atcaagctta ttcaaagaac
     4981 tgacttattt tcgctttcaa tatatcgatc gcgatgcggt ttttcccgcc gcgcggcacg
     5041 ataatgtccg catattgttt agaaggctca atgaattgca ggaacatcgg gcgcacggtt
     5101 ttttgatatt gcgccatcac ggaatccatt gaacgcccac gctcgttaac gtcacgcttg
     5161 atgcggcgca tcaggcagat atccagcggg gtatcaacga aaatggagaa gttaagttcg
     5221 tcacgcaaac gcgcatccgt cagcaacaaa atgccttcga gaatgatgac cttcttcggc
     5281 tcaaccgtca ccgtttcttt catacgcgta tgttcaacat agctgtaaac cggcaggtca
     5341 attgccgagc cgcgtttcaa cgcttgtaaa tgctcaagca acaggctgtg atccatcgcg
     5401 ctgggatggt cgtagttggt cttaacgcgt tcttccatcg acagatggct ttgatctttg
     5461 taatagcagt cttcgggaat tacgccgatg tgttcatcac cgacttgctc acgtaattca
     5521 cgataaaggg tactggcaat aagactcttg ccggaagccg atgcgccagc gataccgata
     5581 atgacgcact gatgagactg atcagtcata tatttagcga cctgattaac ctggatgtta
     5641 ggaagggggc gacgaagcgc caaacgcggc aattataggg atttcatccg tctgatacca
     5701 gtcgaatagc gttgccgcgc gctcagagtt aattgttgac aaagaattcc cggtggcaaa
     5761 ttacgttgat cagttttatg taaggtaaaa aatgttatac gcagttgcgc aaattatccg
     5821 cctttacgtc actttatgag caattcgcat ataaaatgta aaacttttgt actagcataa
     5881 acacagaaac gaatactggc gacctggtct tgcggataaa gcggtaatga gcaaacaatc
     5941 acagcatgta ttaattgccc tgccccaccc gctgcttcac ctggtcagtt taggtttagt
     6001 ctcgtttatc tttacccttt tctcgcttga gctttcgcag tttggcaccc aactcgcccc
     6061 actgtggttc ccgacgtcca tcatgatggt ggcgttttat cgccatgccg ggcgcatgtg
     6121 gccgggaatt gcgctgagct gttcgctggg aaatatcgcc gcatccatcc tgcttttttc
     6181 caccagctcg ctgaacatga cctggacgac catcaatatt gttgaagccg tggtcggggc
     6241 agtgctgcta cgtaaattgc tgccgtggta taaccccttg caaaatctgg ctgactggct
     6301 gcgtctggca ctcggcagcg ccattgttcc gcctctgttg ggggggggtc tggttgtcct
     6361 gctgacggcc ggagacgatc ctctcagggc atttttgata tgggtactgt cagaatccat
     6421 cggcgctctg gcactggtgc cgctgggatt gttatttaaa ccacactatc tgctgcgcca
     6481 tcgcaaccca cggttgcttt ttgagtcgct gctcacgtta gccatcacac tgacgttaag
     6541 ctggctttcg atgctgtatc tgccgtggcc ttttactttc attattgtgc tgttgatgtg
     6601 gagcgccgtg cgcctgccac gaatggaagc ctttttgatc ttccttacca cggtgatgat
     6661 ggtgtcgctg atgatggccg cggatccctc cctgcttgct acgccgcgta catacctgat
     6721 gagccatatg ccgtggctac cgtttttgct gatcctgctg cccgccaaca tcatgacgat
     6781 ggtgatgtat gcctttcgtg cggaacgcaa acacatttcc gaaagcgaaa cccgttttcg
     6841 gaacgccatg gaatattccg ctatcggtat ggcgttagtg ggcaccgagg gacaatggct
     6901 gcaaaccaac aaagcgctct gccagtttct cgggtacagt caggaagagc tgcgcggact
     6961 cacctttcag caactgacct ggccggagga tctcaataaa gatctccaac aggttgaaaa
     7021 gctgataagc ggtgaaataa acacctattc aatggaaaaa cgctactaca accgcaatgg
     7081 cgatgttgtc tgggcgttgc ttgccgtctc actggtgcgc cacacggatg gcacgccgct
     7141 ctattttatc gctcagattg aagacattaa cgagctaaaa cgcaccgaac aggtgaatca
     7201 gcaacttatg gagcgcatca cgctggctaa cgaagcgggc gggattggca tctgggagtg
     7261 ggagttgaag ccgaatattt ttagctggga taagcggatg ttcgagctgt atgaaattcc
     7321 tccacatatc aaaccgaact ggcaggtgtg gtacgagtgc gtgctgccgg aagatcgcca
     7381 gcatgccgaa aaagtgattc gtgattcgtt gcaatcacgc tcgcccttta aactggaatt
     7441 tcgcattacc gtaaaagacg gtattcgcca tatccgcgcc ctcgccaacc gggtactgaa
     7501 taaagaaggc gaagtcgaac gcctgctcgg cattaatatg gatatgactg aggttaaaca
     7561 gcttaacgag gcattgtttc aggaaaaaga gcgcctgcac ataacgctgg actccatcgg
     7621 cgaagccgtg gtctgtattg atatggcaat gaaaattacc tttatgaatc cagtggcgga
     7681 gaagatgagc ggctggacgc aggaagaagc attaggtgtt ccgctcctga cggtgttgca
     7741 tattaccttt ggcgacaatg gtcccctgat ggagaacatt tacagtgccg acacctcacg
     7801 ttccgcgatt gaacaagatg tggtgttgca ctgccggagc ggcggcagct acgacgtgca
     7861 ttacagtatt acgccgttaa gtactctgga cggcagcaat attggttcgg ttctggtgat
     7921 tcaggacgtt accgaatcac gcaaaatgct gcgccagctg agctacagcg cctcccatga
     7981 tgcactgacg catctcgcca atcgcgccag ttttgagaag caactgcgta tgctgctgca
     8041 aacggtaaac agtacacatc agcgacatgc cctggtgttt atcgatcttg atcgctttaa
     8101 agcggtgaat gacagcgccg ggcatgcggc gggcgacgct ttgctgcgcg aactggcgtc
     8161 gttgatgctg agtatgctgc gctccagcga cgtgctggcg cgactcggtg gtgatgaatt
     8221 tggtctactg ttgccagact gtaatatcga aagtgcgcgt tttatcgcta cacgcattat
     8281 cagtgccgtg aatgattatc actttatatg ggaaggccgt gtgcatcggg taggtgccag
     8341 tgccgggatt accttgattg atgacaacaa tcatcaggcg gcagaagtga tgtcgcaggc
     8401 tgatatcgcc tgttatgcct ccaaaaatgg tggacggggc cgggtgacgg tttacgaacc
     8461 acagcaagct gccgcacata gcgaacgggc ggcgatgtcg cttgatgaac agtggcggat
     8521 gattaaagag aatcagttga tgatgatggc ccacggtgtc gcttcgccgc ggatcccgga
     8581 agcgcgtaat ttgtggctga tttcgcttaa actctggagt tgcgaaggcg agattattga
     8641 tgaacaaaaa tttcgtcgta gcttcagcga tccggcactt agccatgctc ttgaccgacg
     8701 ggtattccac gaatttttcc agctggccgc aaaagcggtt gccagtaaag gcataagcat
     8761 cgccctcccc ctttccgttg ccggtttgag tagcgccacg ctggtgaatg atctgcttga
     8821 gcagctggaa aatagccctc taccaccacg gttattacac ctgattattc cggctgaagc
     8881 gattttagat cacgcagaaa gcgtgcaaaa actgcggctg gcgggatgtc ggatagtgct
     8941 cagccaggtg ggccgcgatc tgcaaatctt caactcgctg aaagcgaata tggcagatta
     9001 cctgctactt gatggtgagt tatgcgccaa cgtgcagggt aatttgatgg atgagatgct
     9061 gattacgatt attcaggggc acgctcagcg actcgggatg aaaaccatcg ccgggccagt
     9121 cgttttaccc ttagtgatgg atacgctttc tggcatcggc gtcgatctga tttatggtga
     9181 tgtgattgcc gatgcccaac cgctggattt gctggtgaat agcagttatt tcgcgattaa
     9241 ctgagggagc gtttgcccat tgcctgatgc gacgtatcag gcctacaatt cgtccgttcc
     9301 gtctggttgc cagccttccg tataccagat atgcaacagc gcataagaac gccagggctt
     9361 ccagcgctcg gcataacggc ggatttgtgc cggtgtcatc cccggaaatc gctgtttaat
     9421 cagataatca tccggcagaa aaacatcttt cgcctgccag ccacgcaaag cgaaataatt
     9481 cgccgtccag cgcccgatac ccggaaaagt ttgcagcgtt ttcatcgcct gctccacatc
     9541 gcccggaatt gtcattggta agctgccctc cagcgccgca tttgccagat gaatcagtgc
     9601 ctctgcccgt ttcaacggca tgcctaacgc ttttaatgcc tgcgggtcgg ctgctgccag
     9661 ccgctgaggc gtggggaagc agatatactc cggaaaatca tccagccgtt cgccataaag
     9721 ctgtacaact ttggcagtca attttgccgc catcgccacg ctcaccagtt ggcctaaaat
     9781 cgcccgcaca ccctgctcaa aagcatcaat acagccgggt aaacgcaatc cgggccgcgc
     9841 cgcgcctaac ttgcccaacg caccgttaac aatctgcggg ttacattgca gatcaaacag
     9901 gcggctcatt ttcgccagac actcagcggc aacgggttct aaacctgcac ttaaatttat
     9961 gtgcagagta tggcgggcta tatccggaat agcagtcacc acgccgcgat attcgcccac
    10021 cgccagacta cgggcataat aatcctcagc gaccgtttcc acaccactca ccgcacgggc
    10081 ggcgagaaat cccaacatcc acgaccagtc atacggcggc tgccagttca gggtatacat
    10141 cgcatctcct tattcatccg ctttcagcat aaacgttatt cagacgctac gctttgcttt
    10201 catattccgg ttgtcgcgac ggcaacattt cgctaaagtc acgccccttc ttcaccggca
    10261 tggggattaa ttcgtgttta ttggttttga ttacggtaca gcaaactgtt cagtggcggt
    10321 catgcgtgac ggtaaaccgc aattactgaa aatggaaaac gacagcacgc tgctgccttc
    10381 aatgctttgc gcgccaacgc gtgaagcagt aagtgaatgg ctgtaccgcc atcatgatgt
    10441 tccggcagac gacgatgaaa cgcaggcgct gttacgtcgg gcgattcgtt ataaccgcga
    10501 agaagatatc gatgttacgg cgaaaagcgt gcagttcggt ctttcctcac tggcacagta
    10561 cattgatgat ccagaagaag tgtggtttgt gaaatcacca aaatcgttcc tcggtgccag
    10621 cggcttaaaa ccgcagcagg tagcgctgtt tgaggatctg gtctgcgcaa tgatgttgca
    10681 cattcgccag caggcgcagg cgcagctgcc agaagcgatt actcaggcgg tgattggtcg
    10741 cccgatcaac ttccaggggc tgggcggcga tgaagcaaac gcccaggcgc aagggattct
    10801 ggaacgcgcg gcgaagcgtg ccggattcag ggacgtggta ttccagtacg agccggtcgc
    10861 ggctgggctg gattacgaag ccaccttgca ggaagaaaaa cgggtgctgg tggtggatat
    10921 cggcggtggt acgactgact gttcattgct gctgatgggg ccgcagtggc gttcgcgtct
    10981 cgatcgtgaa gccagcctgc tgggtcacag tggttgccgt attggcggta acgatctgga
    11041 catcgcactg gcgtttaaaa acctgatgcc gttactgggc atgggtggcg aaaccgaaaa
    11101 aggcatcgcc ctgccgattc tgccgtggtg gaatgcggtt gccatcaacg acgtacctgc
    11161 gcagagtgat ttctacagta gtgccaacgg tcgtctgctt aacgatctgg tacgcgatgc
    11221 ccgcgaaccg gaaaaagtgg ccctgctaca gaaagtctgg cgtcagcgtt taagctatcg
    11281 cctggtgcgt agcgcagaag agagcaaaat tgctctttcc agcactgcgg aaacccgcgc
    11341 ctcactgccg tttatcagcg atgaactggc tacgctgatt agccagcaag ggctggaaag
    11401 cgccctcagc cagccactgg cgcggattct ggaacaggtg caactggccc tggataacgc
    11461 ccaggaaaaa ccggacgtta tctacctgac cggcggtagc gcccgttctc cgctgattaa
    11521 aaaagcgctg gcagaacagt tgccgggcat tccgattgca ggcggcgatg actttggctc
    11581 cgtcaccgcc gggctggctc gctgggcgga agtggtattt cgttaaaaga aaatggctca
    11641 gcagcggctt aaatctgcct gcgcctgggc cagtttttct gctgcctgac gtaacggttc
    11701 catcaacggc atggtccgtg ctggagcttg aaggcggaat cgctgcaact ctgtcgcgcc
    11761 gacagtcaac gtcgattcca gccgatgtcg tttggcggcc attttagtca gtaccgcttg
    11821 tctgtctgca ggcgttatcg cttcattcgg cctgaacaca aaacggcgtt cacaactcgc
    11881 tttccagtcg ataaccgcct gggtcagatg gtcgccaaac cctttcactt gcttaacgcc
    11941 acgacgggta acatctgcgg ctgtttcaat accaaaagac cgtaacgccg ctttacgcgc
    12001 agggccaacg ccgggaatag aggcaacatc aataaaaaat ccctccagaa acttctgctt
    12061 ctgccgttcc cttgcagtgt catgaagtgc tgccagagcg cgtttctctt cttccggtaa
    12121 cccgagaatt tcgtccttca ttttttcgag catcgtccgt ttggcgataa aaccttccag
    12181 gccgcctacc tgctggatct ggcggactaa atggtcatag tccattttcg cacgattaaa
    12241 tgcctcacgg cgttgctgaa cttctgcttt gtacgctttg cttgtcagac tgccaataat
    12301 ccagatagcc gccagcacag gaaccaaaat aatataacgc ggttctgcct gaaggccgca
    12361 aagcagcagc gataatgctg acagtgcgat ctcaagcaga atgatgtatt cgcgccgtaa
    12421 caggcctaaa ggaagcggcc tgccagtcgg ttggaaatga tcgggtaatg gcagttgcaa
    12481 tgctggcggt gctactgacg ccatcaccat cgcccagact tttgccagca caaaatcccc
    12541 gccggtggta atgacctctt cgccgagatc aataaaatag ataacacctt gattatccag
    12601 cgcacaccat gggcagtcag ccaaatgacc gggataaaca tgcattgccg aaacggtaca
    12661 tttctttaac tgttggcgca gagaatcaag tgccgctacc cacgctttag ccgtcggacg
    12721 cccggttgcc acgccacttt ccgtgaatgc ctgctgaaac atggcttcaa catcactcgg
    12781 taacatcgat agcggaatcg atcgtggcgg cggttttaaa ccacgtcgct gattatctga
    12841 cgcgtaggca taacggaaat gggtaatatc cgtctccagc gcattacccg catcagagat
    12901 aagcggcaca ccagaataag gatgccggcc accaaacaag acgtgaaaaa tcagcaacgc
    12961 aaggccaaaa ttatcgtgat tttttgtgcg ttcaaagccg acaaatgacg acaaggtttg
    13021 tagctctggc ggcgtaaaat gcgacacgcc gacttcgcat aaatgcagtg tgccattggc
    13081 gttaatctga aaggagtcgc tatcaatcag taccactttg ctgtcgcgac ctaccataaa
    13141 actgttctgg tttacgtcac ccacgacgtg cccgtgctcg tgaaccgtag caaaagatga
    13201 agcaatattg cgcgcaacat agagtagaaa atcccacgca caatgagggt aacgttgacg
    13261 acgatgtgcc gggctataga tcatatgaat cggttcttta ccagaaactt ttggcatcat
    13321 aaaaccgata acttttccgc ctcgtccacc gtgaagcgtt gcctgcggcc aggcgacata
    13381 attcaacaat tgcgcgtcag ctgtcgcagc cataaaggca agtttgtcct gttttaaggc
    13441 gggtggcggc gtgtgataaa tcttggcgac gctatcgaca aactcattga tatcataaac
    13501 cgcgccttca ccgcctttgc ccagttcacg gcctggcgta acgcactcac cagtagcagt
    13561 atataaagtg ggtttcatgg taaccacagc gccagtgcca acgttttatc atcatccgta
    13621 cgctcgttta ccgccgggct ggacaaaaac tgctttaaca attcaggtaa taaatcgagt
    13681 tgttcctgcg ttgctgacgc cagtccattg aaaaacgggg taaagaaagg tacatgagga
    13741 gaattatcca gcatatttaa cgccagtcgc tggataccat ccgtaaatgc tgcaacttta
    13801 tgcacacgct cagtgctggt gaaagtttcc agtctggaaa cggcatcttc atcggtaatg
    13861 aagtgcgtca tattggcata ttcacccacc atcggcgtaa gcggcaattg cagaccgtga
    13921 ccaaaatcca ccaccacgcc gccatcgcca atctgcataa tcaaggtgcc attagccgat
    13981 gaaatcaatc ccagaaacgt gcaggcaaaa tcgcgaactg ctaattcttt ggcttccgcc
    14041 tcggcaaaaa gtcgctgacg aatagtcagc acaatatccg tggcgaggat atcattaagc
    14101 cccaattccc caccctgcac tttttgcgac atataagcca tcgcttcatt gacggcgagc
    14161 atcgcgcctt cgccaccctg cgagacgctg cctgcaccat cagcaagaaa catcaccagt
    14221 aatggttgct gatcgtttaa ccaggcaacc tgcatctggc aggcatcctg acaaggtaaa
    14281 tcagcgctaa tgtgcgacgt tccaacagcg gatgcataaa ccagacgcca gctcacactg
    14341 acgtccagcc ttttggcgct tccagcacaa cttccgttcc cggcgtggag cgggaaaccg
    14401 aacgcagcga actggataac cagctaaaca gttcacggaa ttgtaatcct tgcagaggca
    14461 aaggctgacg aacgctgatt tgtgccagcg tcttcatatc cgccccctga acaccgatgg
    14521 aaaagaaagc aaacctttta tcttcttccc cccgaaacac tttgttggct gcagcctgcc
    14581 actcatcggt tggtgcacca tcggtaatca ggaaaatcca cgggcgataa tacgaaatac
    14641 catttgcacg atactcacgt ttccgctcct ctaccatatc cagggctttg gtaatggccg
    14701 cacccattgg tgtatcgccc tgggcaaaca ggatgggcgg aaaaaaatta gcggcgctgg
    14761 taaagggttg ttccacatgc accgggccga acgtcacaat gccgagttcc actcttttta
    14821 aagccagcgg atcagcaagc agttcatcac gaaaggtaac caatccggcg ttaagttcat
    14881 taataggtct accgttcata gaaccagaaa catccagcag caaaatgcag gggcaacgtg
    14941 gttccgggtt actggcaaaa tcgctggtgg caaacgtgat ttgttcgctc ataagaaatc
    15001 ctttcttaga gaattatcga ggtccggtat cataccgact gcaataatga aataagagca
    15061 aacgaagaaa ctttcagaat taggcccggc ggcgcagaaa attttgctat tcgcctcgca
    15121 agaacggaga gccgtcgatt gacactaagg gcggagtgac ataatttcag gagtgagggt
    15181 tagggagagg tttccccctc cccctggtgt tcttagtaag cctggaagct aatcactaag
    15241 agtatcacca gtatgatgac gtgcttcatc ataacccttt ccttattaaa agccctcttc
    15301 tccgggagag gctttcccgt ttcagcgtcc cgctgaaatc atcggcttac ctcctttcgc
    15361 cctgaatgca gtctatcgct aacgcgtgaa taaaatagtt tcctctgtga ttattgcgta
    15421 acaactcgca aagaatattt gctggcagga tcgcagacta caaagcctgc ggattgacaa
    15481 tcttatcgtg aaggcatact ttcaggagtg agggtagagc ggggtttccc ccgccctggt
    15541 agtcttagta agcggggaag cttatgacta agagcaccac gatgatgagt agcttcatca
    15601 tgaccctttc cttatttaag gccccttcct cgggaggggc tttcccgttt cagcgtcccg
    15661 ctgaaatcgt cggcttacct cctttcgcca tgcacttagt ctatcgctga cttgcagata
    15721 aaatagtttc cagtgtgatt attgcgtaac gtctcgcaaa gaatttttgc tggcaggatc
    15781 gcagacggca aagcctgcgg attgacaatc ttatcgtgaa ggcatacttt caggagtgag
    15841 ggtagagcgg ggtttccccc gccctggtag tcttagtatg ctgggtagct aatcactaag
    15901 agcaccacga tgatgagtag cttcatcatg accctttcct tatttaaggc cccttcctcg
    15961 ggaggggctt tcccgtttca gcatccggct gaaatctgcg gcttacctcc tttcgccctg
    16021 tatgtagtct atcgctaacg cgtagataaa atagtttcct gtgttattac tggatgcgtg
    16081 ctcgcaaatg tgcccgccat tcagacgatt cccgatagtg tttcataatt cctccatttt
    16141 tctcccttat tggctggcta cactagtatc attccgcgaa acgtttcagg aagagaaact
    16201 cttaacgatg aaaggcagtt ataaatcccg ttgggtaatc gtaatcgtgg tggttatcgc
    16261 cgccatcgcc gcattctggt tctggcaagg ccgcaatgac tcccagagtg cagccccagg
    16321 ggcgacgaaa caagcgcagc aatcgccagc gggtggtcgc cgtggtatgc gttccggccc
    16381 attagccccg gttcaggcgg cgaccgccgt agaacaggca gttccgcgtt acctcatcgg
    16441 gcttggcacc attaccgccg ctaatactgt tacggtgcgc agccgcgtgg acggtcaact
    16501 gatggcgtta catttccagg aaggccagca ggtcaaagca ggcgatttac tggcagaaat
    16561 tgaccccagc cagttcaaag ttgcattagc acaagcccag ggccaactgg caaaagataa
    16621 agccacgctt gctaacgccc gccgtgatct ggcgcgttat cagcaactgg caaaaaccaa
    16681 tgtcgtctcc cgtcaggagc tggatgccca acaggcgctg gtcagtgaaa ccgaaggcac
    16741 cattaaggct gatgaagcaa gcgtcgccag cgcgcagctg caactcgact ggagccgcat
    16801 caccgcacca gtcgatggtc gcgttggtct caagcaggtt gatgttggta accaaatctc
    16861 cagtggtgat accaccggaa ttgtggtgat cacccagacg catcctatcg atttagtctt
    16921 taccctgccg gaaagcgata tcgctactgt agtgcaggcg caaaaagccg gaaaaccgct
    16981 ggtggtagaa gcctgggatc gcaccaattc gaagaaatta agtgaaggca cgctgttaag
    17041 cctcgataac caaatcgatg ccacaaccgg tacgattaaa gtgaaagcac gctttaataa
    17101 tcaggatgat gcgctgtttc ccaatcagtt tgttaacgcg cgcatgttag tcgacaccga
    17161 acaaaacgcc gtagtgatcc caacggccgc cctgcaaatg ggcaatgaag gccattttgt
    17221 ctgggtgctg agtagcgaaa acaaggtcag caaacatctg gtgacgccgg gcattcagga
    17281 cagtcagaaa gtggtgatcc gtgcaggtat ttctgcgggc gatcgcgtgg tgacagacgg
    17341 cattgatcgc ctgaccgaag gggcgaaagt ggaagtggtg gaagcccaga gcgccactac
    17401 tccggaagag aaagccacca gccgcgaata cgcgaaaaaa ggagcacgct cctgatgcag
    17461 gtgttacccc cgagcagcac aggcggcccg tcgcgcctgt ttattatgcg tcctgtggcc
    17521 accacgctgc tgatggtggc gatcttactc gccgggatta tcggttatcg cgccctgccc
    17581 gtttcggcgc tgccggaagt ggactatccg accattcagg tggtcacgct ctacccaggt
    17641 gccagcccgg atgtcatgac ctctgccgtt accgcgccgc tcgaacgcca gttcgggcag
    17701 atgtctggcc tgaaacagat gtcgtcgcaa agttccggcg gtgcgtcagt tatcactttg
    17761 cagttccagc taacattacc gctcgatgtc gccgagcagg aagtgcaggc cgcgattaac
    17821 gctgcgacca acttgttgcc gagcgatctg cctaacccgc cggtttacag caaagtgaac
    17881 ccggcagatc cgccgatcat gacgctcgcc gtcacctcaa ccgccatgcc gatgacccag
    17941 gtagaagata tggtggaaac ccgcgtcgcg cagaaaatct cgcagatttc cggtgtcgga
    18001 ctggtgacgc tttccggcgg tcagcgtccg gctgttcgcg tcaaacttaa cgcccaggcg
    18061 attgccgccc tcggcctgac cagcgaaacc gtgcgcaccg ccattaccgg agctaacgtc
    18121 aactcggcaa aaggtagcct cgacggccct tcccgtgcgg tcacgctttc cgctaacgac
    18181 cagatgcaat ccgccgaaga gtatcgccag ctaatcatcg cctaccagaa cggcgcaccg
    18241 attcgtctgg gcgatgtcgc aaccgtagag caaggagcag aaaacagctg gctcggcgcg
    18301 tgggcgaaca aagaacaggc cattgtgatg aatgttcagc gccagcccgg tgctaacatt
    18361 atctccaccg ccgacagcat ccggcagatg ctgccacagc tcaccgagag tctgccgaaa
    18421 tcggtgaagg tgacagtgct ttccgatcgc accaccaata ttcgcgcatc cgtcgatgat
    18481 actcagtttg aattgatgat ggctatcgcg ctggtagtca tgatcatcta cctgtttttg
    18541 cgcaatattc cggcgaccat cattcccggt gttgctgtac cgctgtcgtt aatcggcact
    18601 tttgcggtta tggtgtttct cgatttttca atcaataacc tgacactgat ggcgttaact
    18661 atcgccaccg gattcgtggt cgatgacgcc atcgtagtga tcgaaaacat ttcccgctat
    18721 atcgaaaaag gcgaaaaacc gttggcggcg gcgctcaaag gcgcgggtga aatcggcttt
    18781 accattatct cgctgacctt ctcgctgatt gcggtgttga ttccactgct gtttatgggc
    18841 gatatcgtcg ggcgactgtt ccgcgaattt gctattaccc tggcggtagc gattttgatc
    18901 tcagcggtgg tgtcgctgac cctgacaccg atgatgtgcg cgcggatgct cagccaggag
    18961 tcgctgcgta aacagaaccg attctcccgt gcctcggaaa aaatgttcga caggataatc
    19021 gccgcctatg gtcgtggact ggcgaaggtg ctgaatcatc cgtggctgac cttaagcgtg
    19081 gcgctcagca cgctgctgct tagcgttctc ttatgggtgt tcattccgaa aggtttcttc
    19141 ccggtacagg acaacggcat tattcagggc actttgcagg caccgcaatc cagctccttt
    19201 gccaatatgg cccagcgaca acgccaggtc gcggacgtga ttttgcagga tccggcagtg
    19261 caaagcctga cctcatttgt tggcgttgat ggcactaacc cgtcgctgaa tagtgcacgt
    19321 ttacaaatca acctcaaacc gttggatgaa cgtgatgatc gggtgcaaaa agtcatcgcc
    19381 cgtctgcaaa cggcggtaga taaagtgccg ggcgtcgatc tcttcctgca accaacgcag
    19441 gatctgacta ttgatactca ggtcagccgc acccagtacc agtttacctt gcaggccacc
    19501 tcactggatg cgctcagtac ctgggtgcca cagttgatgg aagaactcca gcaactgcca
    19561 cagctttctg atgtctccag cgactggcag gacaaagggc tggtggcgta tgtcaatgtt
    19621 gatcgcgaca gcgccagccg tctggggatc agcatggcgg atgtcgataa cgccctgtac
    19681 aacgcgtttg gtcagcgact gatttccact atttatactc aggctaacca gtatcgcgta
    19741 gtgctggagc acaacaccga aattaccccg ggcctcgcgg cgctggatac cattcgcctg
    19801 accagtagcg acggcggcgt ggtgccgtta agctcaattg ccaaagttga acagcgtttt
    19861 gcgccgcttt ccatcaacca tctggatcag ttcccggtaa cgaccatctc ctttaacgtg
    19921 ccggataact attcgctggg cgatgcggtg caggcgatta tggacaccga aaagacgctg
    19981 aatctaccgg tggatatcac cacgcaattc cagggcagca ccctcgcctt ccagtcggcg
    20041 ctgggcagca ctgtctggct gattgtcacg gcggtggtgg cgatgtatat cgtgctcggc
    20101 attctgtacg agagctttat tcacccgatc accattctct cgacgctacc caccgcagga
    20161 gtcggcgcac tgctggcgtt aatgattgcc ggtagcgaac tggatgtgat tgcgattatc
    20221 ggcattattt tgctgatcgg tatcgtgaag aagaacgcca tcatgatgat cgacttcgcg
    20281 ctggctgctg agcgcgagca aggcatgtcg ccgcgcgatg ccatctacca ggcttgtctg
    20341 ttgcgttttc gtccgatcct gatgaccact ctggcggctc tgcttggcgc gctgccgctg
    20401 atgttgagta ccggggtcgg cgcggaactg cgtcgtccgt taggtatcgg catggtcggc
    20461 ggcctgattg tcagccaggt gctgacgctg tttaccacgc cggtgattta tctgctgttc
    20521 gaccgcctgg cattgtggac caaaagtcgc tttgctcgcc atgaagagga ggcgtaagtg
    20581 aagttttttg ccctcttcat ttaccgtccg gtggcgacga ttttactgtc ggttgccatt
    20641 accctgtgcg gcatactggg cttccgtatg ctgtcggtcg ccccgctgcc gcaggtcgat
    20701 tttccggtga ttatggtcag cgcctcgctg cccggtgcgt caccagaaac aatggcgtct
    20761 tccgttgcca cgccgctgga gcgctcactt gggcgcattg ccggagtcag tgaaatgacc
    20821 tccagcagtt cgctcggcag cacgcgtatt attttgcagt ttgattttga ccgggatatc
    20881 aacggcgcgg cgcgcgatgt gcaggcggcg atcaacgctg cacaaagttt gctgcccagc
    20941 gggatgccca gccgtccgac ctatcgcaaa gctaacccgt cggatgcgcc aattatgatc
    21001 ctcacgctga catccgatac ttattcgcag ggtgaactgt acgattttgc ctcgacgcag
    21061 ctggctccga cgatttcgca aatcgacggt gtcggtgatg tcgatgttgg cggcagctcg
    21121 ctgcccgccg tacgcgtcgg gctgaatccg caggcgctgt ttaatcaggg cgtctcgctg
    21181 gacgacgtac gcaccgccat cagcaatgcc aacgtgcgta aaccgcaggg ggcgctggaa
    21241 gatggcactc accgctggca gatccagacc aatgatgagc taaaaaccgc cgccgaatat
    21301 cagccgttga ttattcatta caacaacggc ggcgcggttc gtctgggcga tgtggcgacg
    21361 gtgaccgact cggtgcagga tgtgcgcaac gccgggatga ccaacgccaa accggctatt
    21421 ttgctgatga tccgcaagct gccggaagcc aatattatcc agacggtaga cagcatccgg
    21481 gcaaaattac cggagttgca ggaaaccatt ccggcggcga ttgatctgca aattgctcag
    21541 gatcgctccc ccaccattcg cgcctcgctg gaagaagtcg agcaaacgct gattatctcg
    21601 gtggcgctgg tgattctggt ggtgttttta ttcctgcgct cgggtcgcgc cactattatt
    21661 cccgccgttg cggtgccggt ttcgctgatt ggtacgtttg cggcgatgta cctgtgcgga
    21721 ttcagtctca ataacctttc gttaatggcg ctcaccatcg ctactggttt cgtggtggat
    21781 gacgccatcg tggtgctgga aaacattgcc cgtcatctgg aagcggggat gaaaccgcta
    21841 cagaccgcgc tacaaggtac tcgcgaagtc ggctttacgg tgctgtcgat gagtctgtca
    21901 ctggtggcgg tgttcctgcc gctgctgttg atgggcggat tgccgggccg actgttacgc
    21961 gaatttgccg tgacgctttc tgtcgccatt ggtatttcgt tgctggtttc tctgacatta
    22021 acgccaatga tgtgtggctg gatgctgaaa gccagcaagc cgcgcgagca aaagcgactg
    22081 cgtggttttg gtcgcatgtt ggtagccctg caacaaggct acggcaagtc actgaactgg
    22141 gtgctcaatc atacccgtct ggtgggcgtg gtgctgcttg gcaccattgc gctgaatatc
    22201 tggctgtata tctcgatccc gaaaaccttc ttcccggagc aggacactgg cgtgttgatg
    22261 ggcgggattc aggcggatca gagtatttcg tttcaggcga tgcgcggcaa attgcaggat
    22321 ttcatgaaaa ttatccgtga cgatccggca gtggataatg tcaccggctt taccggcggt
    22381 tcacgagtta acagtgggat gatgtttatc accctcaagc cacgcgacga acgcagcgaa
    22441 acggcgcagc aaattatcga ccgtctgcgc gtaaaactgg cgaaagaacc gggggcgaat
    22501 ctgttcctga tggcggtaca ggatattcgc gttggtgggc gtcagtcgaa cgccagctac
    22561 cagtacacgt tgttatccga cgacctggcg gcactgcgcg aatgggagcc gaaaatccgc
    22621 aaaaaactgg cgacgttgcc ggaactggcg gacgtgaact ccgatcagca ggataacggc
    22681 gcggagatga atctggttta cgaccgtgac accatggcac ggctgggaat cgacgtacaa
    22741 gccgccaaca gtctgttaaa taacgccttc ggtcagcggc aaatctcgac catttaccag
    22801 ccgatgaacc agtataaagt ggtgatggaa gtggatccgc gctataccca ggacatcagt
    22861 gcgctggaaa aaatgttcgt tatcaataac gaaggcaaag cgatcccgct gtcgtatttc
    22921 gctaaatggc aaccggcgaa tgccccacta tcggtgaatc atcagggatt atcggcggcc
    22981 tcgaccattt cgtttaacct gccgaccgga aaatcgctct cggacgccag tgcggcgatc
    23041 gatcgcgcaa tgacccagct tggtgtgcct tcgacggtgc gcggcagttt tgccggcacg
    23101 gcgcaggtgt tccaggagac gatgaactcg caggtgatcc tgattatcgc cgccatcgcc
    23161 acggtgtata tcgtgctggg tatcctttac gagagttacg tacatccgct gacgattctc
    23221 tccaccctgc cctcggcggg cgttggagcg ctgttggcgc tggagctgtt caatgccccg
    23281 ttcagcctaa tcgccctgat agggatcatg ctattaatcg gcatcgtgaa gaaaaacgcc
    23341 attatgatgg tcgattttgc gcttgaagcc caacggcacg gtaacctgac gccgcaggaa
    23401 gctattttcc aggcctgtct gctgcgtttt cgcccgatta tgatgaccac cctggcggcg
    23461 ctgtttggtg cgctgccgct ggtattgtcg ggcggcgacg gctcggagct gcggcaaccc
    23521 ctggggatca ccattgtcgg cggactggta atgagccagc tccttacgct gtataccacg
    23581 ccggtggtgt atctcttttt cgaccgtctg cggctgcgtt tttcgcgtaa acctaaacaa
    23641 acggtaaccg agtaaatgac agatcttccc gacagcaccc gttggcaatt gtggattgtg
    23701 gctttcggct tctttatgca gtcgctggac accaccatcg taaacaccgc ccttccctca
    23761 atggcgcaaa gcctcgggga aagtccgttg catatgcaca tggtcattgt ctcttatgtg
    23821 ctgaccgtgg cggtgatgct gcccgccagc ggctggctgg cggacaaagt cggcgtgcgc
    23881 aatattttct ttaccgccat cgtgctgttt actctcggtt cactgttttg cgcgctttcc
    23941 ggcacgctga acgaactgtt gctggcacgc gcgttacagg gcgttggcgg cgcgatgatg
    24001 gtgccggtcg gcagattgac ggtgatgaaa atcgtaccgc gcgagcaata tatggcggcg
    24061 atgacctttg tcacgttacc cggtcaggtc ggtccgctgc tcggtccggc gctcggcggt
    24121 ctgctggtgg agtacgcgtc gtggcactgg atctttttga tcaacattcc ggtggggatt
    24181 atcggtgcga tcgccacatt gctgttaatg ccgaactaca ccatgcagac gcggcgcttt
    24241 gatctctccg gatttttatt gctggcggtt ggcatggcgg tattaaccct ggtgctggac
    24301 ggcagtaaag gtacaggttt atcgccgctg acgattgcag gcctggtcgc agttggcgtg
    24361 gtggcactgg tgctttatct gctgcacgcc agaaataacc accgcgctct tttcagtctg
    24421 aaactgttcc gcactcgtac cttttcgctg ggcctggcgg ggagctttgc cggacgtatt
    24481 ggcagtggca tgttgccctt tatgacaccg gttttcctgc aaattggcct cggtttctca
    24541 ccgtttcatg ccggactgat gatgatcccg atggtgcttg gcagcatggg aatgaagcga
    24601 attgtggtac aggtggtgaa tcgctttggt tatcgtcggg tactggtagc gaccacgctg
    24661 ggtctgtcgc tggtcaccct gttgtttatg actaccgccc tgctgggctg gtactacgtt
    24721 ttgccgttcg tcctgttttt acaagggatg gtcaactcga cgcgtttctc ctccatgaac
    24781 accctgacgc tgaaagatct cccggacaat ctggcgagca gcggcaacag cctgctgtcg
    24841 atgattatgc aattgtcgat gagtatcggc gtcactatcg ccgggctgtt gctgggactt
    24901 tttggttcac agcatgtcag cgtcgacagc ggcaccacac aaaccgtctt tatgtacacc
    24961 tggcttagca tggcgttgat catcgccctt ccggcgttca tctttgccag agtgccgaac
    25021 gatacgcatc aaaatgtagc tatttcgcgg cgaaaaagga gcgcgcaatg aagttctggc
    25081 gacccggtat taccggcaaa ctgtttctgg cgattttcgc cacctgcatt gtcttgctga
    25141 tcagtatgca ctgggcggtg cgtatcagtt ttgagcgtgg ctttattgat tacatcaagc
    25201 atggtaatga acagcgatta caactgttaa gtgatgcgct tggcgagcag tatgcgcagc
    25261 atggcaactg gcgcttcctg cgcaacaatg atcgctttgt ctttcagatc ctgcgttcat
    25321 ttgaacacga taattcggaa gataaacccg gcccgggtat gccaccgcac ggctggcgta
    25381 cccagttctg ggtggttgat caaaacaaca aagtgctggt tggtccgcga gcgccgattc
    25441 cacctgacgg tacacggcga cccattctgg tcaacggtgc ggaagttggc gcggtgatcg
    25501 cctcccccgt tgagcggtta acgcgcaata ctgatatcaa tttcgataaa caacagcggc
    25561 aaaccagctg gttgattgtc gctctggcaa cgttactcgc ggcgctcgcc acctttctgc
    25621 tggcgcgcgg tttactggca ccggtaaaac gacttgtcga tggcacgcac aaactggcgg
    25681 cgggcgattt cactacccgc gtaacgccca ccagtgaaga tgaactgggc aaactggcgc
    25741 aagacttcaa ccagcttgcc agcacactgg agaaaaacca gcaaatgcgg cgcgatttta
    25801 tggccgatat ttctcacgaa ctgcgtacgc cattagcggt gctgcgcggt gaactggaag
    25861 ccattcagga tggcgtgcgt aaattcacgc cggagacggt ggcgtcttta caggcggagg
    25921 tcggtacact gaccaaactg gttgacgatc tccatcagtt gtcgatgtct gatgaaggcg
    25981 ctctcgccta tcaaaaagca ccggtagatt tgatcccact gctggaagtg gcgggcggcg
    26041 catttcgcga acgattcgcc agtcgtggcc tgaaactgca attttccctg ccagacagta
    26101 ttaccgtatt tggcgatcgc gaccgtttaa tgcagttatt caataactta ctggaaaaca
    26161 gcctgcgcta cactgacagc ggcggcagcc tgcaaatctc tgccgggcag cgcgacaaaa
    26221 cggtgcgcct gacctttgcc gacagtgcgc caggtgtcag tgacgatcag ctacaaaaat
    26281 tgtttgaacg tttttatcgc accgaaggtt cccgcaaccg tgccagcggc ggttccgggc
    26341 tggggctggc gatttgcctg aacattgttg aagcacataa tggtcgcatt attgctgccc
    26401 attcgccttt tggcggggta agcattacag tagagttacc gctggaacgg gatttacaga
    26461 gagaagtatg accgagttac caatcgacga aaacacaccg cgtattttga tcgtggaaga
    26521 tgaaccgaag ctggggcagt tgctcattga ttatctgcgt gctgcgcgct atgcgccgac
    26581 gcttatcagc cacggcgatc aggtactgcc gtatgtgcgc cagacaccac cggatctgat
    26641 cctgttagat ctgatgctcc ctggcaccga tggcctgacg ctgtgccggg aaattcgtcg
    26701 tttttctgac attccgatcg tgatggtgac ggcaaaaatc gaagagatcg atcgcctgct
    26761 ggggctggag attggcgcag atgattatat ctgtaagccg tacagcccac gggaagtggt
    26821 agcgcgcgtc aaaaccattt tgcgccgttg caaaccgcag cgcgagttgc agcaacagga
    26881 tgctgaaagc ccgttgatta tcgacgaagg tcgttttcag gcttcatggc gcggtaaaat
    26941 gcttgacctg acacctgcgg aatttcgtct gttgaaaacg ctctctcatg aaccaggaaa
    27001 agtgttctcc cgcgagcaat tgctcaatca tctttatgac gactaccgcg tagtaaccga
    27061 ccgcaccatt gacagccaca ttaaaaacct gcgccgcaag ctggaatctc tcgacgccga
    27121 acagtcattt atccgcgccg tttacggcgt cggttaccgc tgggaagccg acgcctgccg
    27181 catcgtttag ttttagcgac attattttgt tagccggaga tggcgttcag acccgatccg
    27241 gcaacattat cccacgcatg gtcagcaaac tgacattatc tctccgccct ggcaggcatc
    27301 agaaaatcgc tcatacttta atcggtaaac agcaccttta gatgctgttt tcgatacaca
    27361 atttcaatca aggagtcatt atggctggtt ggtttgaact cagtgagagc agtgataatc
    27421 agttccggtt tgtgctaaaa gcgggcaatg gtgagactat cctcaccagc gagctgtata
    27481 cctcaaaagc ctctgcggaa aagggcatcg cgtcggtgcg tagcaacagc ccgcaagaag
    27541 aacgctatga gaaaaaaacg gcaagtaacg gcaaattcta tttcaatctg aaagccgcta
    27601 atcatcaaat tatcggctcc agccagatgt acgccaccgc gcaatctcgt gaaaccggaa
    27661 ttgcctccgt taaagccaat ggcacaagcc agacggtgaa agacaatacg taattacgat
    27721 gccgggcgtg ggttgttggc gtccggcttt atatccaaat aacgcttagt tattaataat
    27781 taagagtaac cgccgccatt aaaatagtgg gcactgacga gcactcgttg tgctttcttt
    27841 gtttaccatc ccatacttat cctcctgaac atgttcatgt gccaaaatat cttctaatac
    27901 tttcgtgacg ctatccggtt tatgacatac actgatgtaa gatttttact tacaggcggg
    27961 cgtaaaatgg ccagaacgat gacggttgat ctcggcgatg agttgcgtga atttatcgag
    28021 tccttaattg aatccggcga ctatcgcacc caaagcgaag tgatccgtga gtcactgcga
    28081 ttacttcgag aaaagcaagc agagtctcgc ctgcaagcgt tacgtgacat gctcgcagaa
    28141 gggttgagta gtggtgaagc tcagccgtgg gaaaaggacg cgttcttacg gaaggtgaaa
    28201 gcagggataa ggaaataatg tgcgtattat taaactcatg ccaaaagcca atgaggattt
    28261 agaaggcatc tggtattaca gctatcacca tttcggcgaa ccacaagccg acagatatgt
    28321 ggaacatctt tcagacgttc tccagattct gagtaacaat aatataggta cgcccagacc
    28381 tgaattaggc gaaggtattt ttgttcttcc ttttgaacgc cacgtcattt atttcctgca
    28441 atcaccaggg gaaattatcg tcattcgtat ccttaaccaa aaccaggacg ccaccagaca
    28501 tttacactgg agctaaaccc gccgattgcg ctacaatgcc cgcccttaaa gtgggggcac
    28561 tcccctaacc gcttcatcag gtgaagcgga tctgacctgt catcagaacg agagaattat
    28621 gtttaaaccg gaactccttt ccccggcggg aacgctgaaa aatatgcgtt acgctttcgc
    28681 ttatggcgca gatgctgttt atgcgggcca gccgcgttat tccctgcgtg tgcgcaacaa
    28741 cgaattcaac cacgaaaatc ttcagctcgg catcaatgaa gcccacgcgc tggggaaaaa
    28801 gttttatgtc gtggtcaaca ttgcaccgca caacgccaag ctgaaaacct ttatccgtga
    28861 cctgaaaccg gtggtggaaa tggggccgga tgcgctgatt atgtccgatc cagggctgat
    28921 tatgctggtg cgtgagcact tccctgaaat gccgatccac ctttcggtgc aggctaacgc
    28981 cgtgaactgg gcgacggtga aattctggca gcaaatgggc ctgacccgcg tgatcctctc
    29041 tcgcgagctg tcgctggaag agattgaaga gatccgcaat caggtgccgg atatggagat
    29101 cgagatcttc gttcacggcg cgctgtgcat ggcctactcc ggtcgctgcc tgctctctgg
    29161 ctatatcaac aagcgcgacc cgaaccaggg cacctgcacc aacgcctgcc gctgggagta
    29221 caacgtccag gaagggaaag aagatgatgt tggcaacatc gtacacaagt acgagccgat
    29281 tccggtgcaa aatgttgagc cgacgctggg tatcggcgca ccaaccgaca aagtgtttat
    29341 gatcgaagag gcccagcgtc cgggcgagta tatgaccgcg tttgaagatg agcacggcac
    29401 ttacatcatg aactcgaaag atctgcgcgc catcgcccat gtagaacgcc tgaccaaaat
    29461 gggcgtgcat tcgctgaaaa tcgaaggtcg taccaaatct ttctactatt gtgcacgcac
    29521 cgcacaggtt taccgcaaag ctatcgatga cgccgctgcg ggaaaaccgt tcgataccag
    29581 cctgctggaa actctggaag gtctagcgca tcgtggctat accgaaggtt tcctgcgtcg
    29641 tcatactcac gacgattatc agaactacga atacggttat tcagtttctg accgccagca
    29701 gtttgttggt gagtttaccg gtgagcgcaa gggggacctc gcggcggtag cggtgaaaaa
    29761 taaattctcc gttggcgaca gccttgagct gatgacgccg caaggcaaca ttaattttac
    29821 ccttgagcac atggaaaacg ccaaaggcga agctatgccg atagcaccag gcgatggtta
    29881 tactgtgtgg ctcccggtcc cgcaggatct tgagctcaat tacgcgctgc tgatgcgtaa
    29941 tttctccggg gaaaccacgc gtaatcccca cggtaagtga ttaatttcga ttatttttcc
    30001 cggatggaaa attcttagaa accgatcaca tacagctgca tttattaagg ttatcatccg
    30061 tttcgctgaa aaacataacc cataaaatgc tagctgtacc aggaaccacc tccttagcct
    30121 gtgtaatctc ccttacacgg gcttattttt tatgcataag ccctatccct ggtcaccgtc
    30181 ttccattgac cacatcgata gaatctccct tcatagcacg atgcctttca cgtaacggca
    30241 tcgtgctcgc acaggttccg gctaagcaca accagaacgc gcatgtttga cgcttaccaa
    30301 aaaatattct cactctccac atttgaatgt cagacgagcg acgccatgta atcctgcacc
    30361 ttctgtcttc aggtcaacta tctgcatttt tttgccctga gtaacacaga aatgggctgc
    30421 atcatttttt actatatttt ctgcaccaga tattctgccc ctggctaaag aagcttcggc
    30481 ttcggtgtag tattggttat cgagtttacg ctgaatatta cttttatatg caagaccaaa
    30541 tttaccgata cttgtctcat cattatgcac agcacaacca gacacaataa aaatactaat
    30601 taatgatata gcaactatct ttttcatctc accttccccc attaaatacc aacgacactc
    30661 tctag
//
LOCUS       DAPGOF010000048        29574 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.048, whole genome shotgun sequence.
ACCESSION   DAPGOF010000048 DAPGOF010000000
VERSION     DAPGOF010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29574
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.048"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..487)
                     /gene="sulA"
                     /locus_tag="RHV13_001824"
     CDS             complement(<1..487)
                     /gene="sulA"
                     /locus_tag="RHV13_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HDV1241364.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLPPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LK"
     gene            706..1335
                     /gene="sxy"
                     /locus_tag="RHV13_001825"
     CDS             706..1335
                     /gene="sxy"
                     /locus_tag="RHV13_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HDV1241365.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     NGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(1298..3460)
                     /gene="yccS"
                     /locus_tag="RHV13_001826"
     CDS             complement(1298..3460)
                     /gene="yccS"
                     /locus_tag="RHV13_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HDV1241366.1"
                     /translation="MAYMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(3470..3916)
                     /locus_tag="RHV13_001827"
     CDS             complement(3470..3916)
                     /locus_tag="RHV13_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HDV1241367.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TRLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            4039..6093
                     /gene="helD"
                     /locus_tag="RHV13_001828"
     CDS             4039..6093
                     /gene="helD"
                     /locus_tag="RHV13_001828"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HDV1241368.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQHRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVLIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(6125..6583)
                     /gene="mgsA"
                     /locus_tag="RHV13_001829"
     CDS             complement(6125..6583)
                     /gene="mgsA"
                     /locus_tag="RHV13_001829"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDV1241369.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(6679..7341)
                     /locus_tag="RHV13_001830"
     CDS             complement(6679..7341)
                     /locus_tag="RHV13_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HDV1241370.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            7514..7927
                     /locus_tag="RHV13_001831"
     CDS             7514..7927
                     /locus_tag="RHV13_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDV1241371.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(7972..8289)
                     /gene="hspQ"
                     /locus_tag="RHV13_001832"
     CDS             complement(7972..8289)
                     /gene="hspQ"
                     /locus_tag="RHV13_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HDV1241372.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(8347..9537)
                     /gene="rlmI"
                     /locus_tag="RHV13_001833"
     CDS             complement(8347..9537)
                     /gene="rlmI"
                     /locus_tag="RHV13_001833"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HDV1241373.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGPVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCNQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            9632..9910
                     /gene="yccX"
                     /locus_tag="RHV13_001834"
     CDS             9632..9910
                     /gene="yccX"
                     /locus_tag="RHV13_001834"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDV1241374.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(9907..10236)
                     /gene="tusE"
                     /locus_tag="RHV13_001835"
     CDS             complement(9907..10236)
                     /gene="tusE"
                     /locus_tag="RHV13_001835"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HDV1241375.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(10327..10986)
                     /gene="yccA"
                     /locus_tag="RHV13_001836"
     CDS             complement(10327..10986)
                     /gene="yccA"
                     /locus_tag="RHV13_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HDV1241376.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(11193..11280)
                     /locus_tag="RHV13_001837"
     tRNA            complement(11193..11280)
                     /locus_tag="RHV13_001837"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11244..11246),aa:Ser,seq:tga)
     gene            11706..12824
                     /gene="hyaA"
                     /locus_tag="RHV13_001838"
     CDS             11706..12824
                     /gene="hyaA"
                     /locus_tag="RHV13_001838"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HDV1241377.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            12821..14614
                     /gene="hyaB"
                     /locus_tag="RHV13_001839"
     CDS             12821..14614
                     /gene="hyaB"
                     /locus_tag="RHV13_001839"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HDV1241378.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPTTWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            14633..15340
                     /gene="hyaC"
                     /locus_tag="RHV13_001840"
     CDS             14633..15340
                     /gene="hyaC"
                     /locus_tag="RHV13_001840"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HDV1241379.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            15337..15924
                     /locus_tag="RHV13_001841"
     CDS             15337..15924
                     /locus_tag="RHV13_001841"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HDV1241380.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            15921..16319
                     /gene="hyaE"
                     /locus_tag="RHV13_001842"
     CDS             15921..16319
                     /gene="hyaE"
                     /locus_tag="RHV13_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HDV1241381.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            16316..17173
                     /locus_tag="RHV13_001843"
     CDS             16316..17173
                     /locus_tag="RHV13_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HDV1241382.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVNALLNELQHQLSQRQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            17307..18851
                     /gene="appC"
                     /locus_tag="RHV13_001844"
     CDS             17307..18851
                     /gene="appC"
                     /locus_tag="RHV13_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HDV1241383.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            18863..19999
                     /gene="appB"
                     /locus_tag="RHV13_001845"
     CDS             18863..19999
                     /gene="appB"
                     /locus_tag="RHV13_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDV1241384.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARDDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            20012..20104
                     /gene="cbdX"
                     /locus_tag="RHV13_001846"
     CDS             20012..20104
                     /gene="cbdX"
                     /locus_tag="RHV13_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HDV1241385.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            20184..21482
                     /gene="appA"
                     /locus_tag="RHV13_001847"
     CDS             20184..21482
                     /gene="appA"
                     /locus_tag="RHV13_001847"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HDV1241386.1"
                     /translation="MKAILIPFLSLLIPLTPQSTFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLTKKGCPQP
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVSRSRATPLLDLIMTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(21602..23782)
                     /gene="etk"
                     /locus_tag="RHV13_001848"
     CDS             complement(21602..23782)
                     /gene="etk"
                     /locus_tag="RHV13_001848"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HDV1241387.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDMVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGSFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(23802..24248)
                     /gene="etp"
                     /locus_tag="RHV13_001849"
                     /gene_synonym="yccY"
     CDS             complement(23802..24248)
                     /gene="etp"
                     /locus_tag="RHV13_001849"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HDV1241388.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(24236..25375)
                     /locus_tag="RHV13_001850"
     CDS             complement(24236..25375)
                     /locus_tag="RHV13_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HDV1241389.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(25421..27517)
                     /locus_tag="RHV13_001851"
     CDS             complement(25421..27517)
                     /locus_tag="RHV13_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HDV1241390.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQLEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGKSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(27517..28263)
                     /locus_tag="RHV13_001852"
     CDS             complement(27517..28263)
                     /locus_tag="RHV13_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HDV1241391.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAVKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(28260..28904)
                     /locus_tag="RHV13_001853"
     CDS             complement(28260..28904)
                     /locus_tag="RHV13_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HDV1241392.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALTIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLHIAGRDV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(29011..29316)
                     /locus_tag="RHV13_001854"
     CDS             complement(29011..29316)
                     /locus_tag="RHV13_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1241393.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
BASE COUNT         7159 a         7525 c         7791 g         7099 t
ORIGIN      
        1 tttttagccc ggaaagttgt ctcgttgcgt gagaggatgc gcttaccgga cgcataataa
       61 accccatcgc gttaccttca tttgccgcat caacaagttc agcatgctct tcttcagtca
      121 aatcatctgc caaccaaccg atcaccacac tgtaattgcc cgtgcgtaaa gcgcgaacca
      181 ttgactccac agtgtggcaa ggggggagct ggctaatctg cattactttc gttaagggta
      241 gcccagatgc ctgaacccat tcccgactca gtttttgttg cggtgttaac cagagttgcc
      301 agcgcgattg ctgaccgagt tgctgtaaca atggcaacag tagaagttgc gtcatcatgg
      361 gctgatcttc gcgatagaca acttcactga taagcccggc tgtagtgttt tccgtagaga
      421 cacgcgcaat tttacttgct gcggatgaga acgacgaaga acgatgtgca tagcctgaag
      481 tgtacataat caatccagcc cctgtgagtt actgtatgga tgtacagtac atccagtgac
      541 aacaaagatc aaccctattt tcggaaagag cctcgcaaat tttgtcgtta gtgacgggaa
      601 aacataaatt aatcttgccc cttaagaata agttgcctat tttcgtattt aacggatccg
      661 ttaatgtgaa tcattctttt atgttatgat tttaaaagga attttatgaa aagcctctcc
      721 tataagcgga tctataaatc acaagaatac ctggcaacgt tgggcacaat tgaataccga
      781 tcattgtttg gtagttacag cctgaccgtt gacgacacgg tatttgcgat ggtttctaat
      841 ggtgagttgt atcttcgggc ttgtgagcaa agtgcacagt actgtgtaaa acatccgcct
      901 gtctggctga catataaaaa gtgtggccga tccgttaccc tcaattacta tcgggttgat
      961 gaaagtctat ggcgaaatca actgaagctg gtgcgtctgt cgaaatattc tctcgatgca
     1021 gcgctgaaag agaaaagcac gcgcaatacc cgggaaagac tgaaagattt gcccaatatg
     1081 tcttttcatc tggaagcgat tctcggggag gtggggatta aggatgtacg ggcgttacgt
     1141 atacttgggg caaaaatgtg ttggttgcga ctgcggcagc aaaacagtct ggtgacagaa
     1201 aagattctgt ttatgcttga aggtgccatt atcggcattc atgaagctgc gctcccggtg
     1261 gcacgccgcc aggagcttgc agaatgggct gactctctta cgccgaaaca ggagtttcct
     1321 gcggaacttg agtaatctgg cgttgcagac gaccaatctc aggcagtaat gcaatcaata
     1381 acccaacttg ttgtacgacc agaggttctt tgctgtctgc ccgtggttca agttgctgca
     1441 tccgctgttt caggccagct aatgcctgat tgacgcgttc ttcatcagca ggttgatgat
     1501 gtaacgcgtc atcaacatag caaactgcgt catcaagaaa cgccagaatt tcaggattag
     1561 ttaactgctc ccggtgagca ccgagggctg agatatagct ggtaaacgta tggttaaggc
     1621 acagcaaccg aaacgcggct tcgcgaattt gcggggtaac gttcggttcg ctggacatat
     1681 ttgataccac cgacgccagt tcagcatcac ggttgtgtgc atcgcggcgg gcaatacgat
     1741 acgccagacg gttatcacgc ccctgatggt attgctccag tatggcatcg agatagcgac
     1801 agttggcctc tgtggcgcgt tcgagcatgc gcggcagatt gcgaaactgc cagtcaggcc
     1861 agatgtagct cactgccgcc cacgcaatgg cacaaccaat cagcgtatcg attacgcgag
     1921 gtaacgctac ttcaaaacct tcacccagta agttaaaaca cagtagcacc aaaagtgtga
     1981 tgaacatcgt tgcatgagcg tattgcacgt tacggaaggc aaaaaatagc acgccggtaa
     2041 taaccagcag caccagctgc ccttccagtg atggcacaaa ccacagcaca ggaatgccaa
     2101 tggcgatacc taccagcgta ccaataatcc ttaacttcag gcggtggcgc gtggcgttat
     2161 agtttggctg gcagacaaac aaacttgtca gcaagatcca atacccgtga tgcattccgg
     2221 ttatttgaat gatggcgtag ccgaagcaca acaccagcga cattcttacc gcatgacgga
     2281 agagggcgga ttccggcgtg aagtgacggc taagacgcag ccagatatca ctcaatccgt
     2341 gcgggctgtc atcagcgagc tcattttcgt cattattatg gggcagtgcc tgggcctgtt
     2401 ctgattcaat tgttgccagt tgggcatcaa tggcgcgtaa attgttcagc aaaaatccca
     2461 gtgttttgag taaatcggca ggcgcgccgt tatcgcgcat ccgctccagg gcagcatcaa
     2521 tatgcgtaaa agcgcgctca aaatgcggat catgttgata aggctgacgc agcaaaatac
     2581 agcgtgacag ttgctggcac gcctggccct gcatcgacat cagccgctga aaacggaaca
     2641 gcacgtcgct gtggcgaaaa tgttcacgca atgtttgata ctgaatatga gaagagctgg
     2701 cacgctcgtg aatatcctgt gcgacaaagt aataatgcag cgtgcgacgc gttccccgtt
     2761 gaccacgatc gccacgtaag cgggtcagca gcgagagttt cgtctgattc aatgtcgcca
     2821 tcagctgacc gttggcgaga gccaaatcgt acagcggtgc ctggctttca tcttcaatat
     2881 caggatcaaa catgcgcgac ttgagctcaa gataacgcgc cagttgttca tagcaacgcg
     2941 ccaggttgtc ctgcagcggg cggaccggga acagcagatg accaataagt gtcaggacgt
     3001 tgtaccagac ggcaccggcc agcagataca tcggctgctg ataccagtgc tcatacagtg
     3061 atgttcccaa cattgtgtaa atggcgatca gcaatgcacc gaaggcaatt gttgcatagc
     3121 gttgacccag accgccgagc aaaatgaagc cgctggtaga gagcgttaag ccaatcgcaa
     3181 atagccaggg ccagggaaac agcaattcta ctgaggccga ggcgataaaa aagcagaaca
     3241 gcgtaatgat gaggttacgc aaacgtcccg ccagtcggtc atcgagatcg gtcagcgccg
     3301 ctgccaccat ccccagcgtc agcggaatcg tcagttttac atcacccagc caccacggaa
     3361 acgctgtggt tccacaaagc gcaataaaaa tacgcgcgta atacagccag gcgctgttcc
     3421 aggtatagcg tttgagcaaa ggacttagca tataggccat attagtggat tattcaaaac
     3481 gacgacgtgc attggcttca cgcgcagctt gcgctgtttc taccgatacc acgcgacgac
     3541 caaccggcca tagtgcaata gcggcaattt taaagttcgc aataccgaca ggaatgccaa
     3601 tgatggaaat acattgtgcg atgcccgttg caatgtgcat caggcataac caccagccaa
     3661 agaaaatcaa ccagaaaata ttcaataccg taccgccagt attcagcagc acatttttgc
     3721 cagccgggtt cagttcatcg acatgaatag cttcattgcc ataaggcacc agagacagtc
     3781 tagtgatctc ccagcaggat cgtgtcagcg gtaaggtaaa aatcagcaca atactgacca
     3841 gagtcgccaa cagccagccc agagtggtgg caaatccgcc aagcacaaag ttcagaatgt
     3901 tcaaaacggt acgcataaaa cctcgcttta ctgtggtttt cagtaatggg cggcaattgt
     3961 aacgtttttt tgggctggag cacgttttct ctgacggtta cactgataag aaataatttc
     4021 gtgtggatct acagagtcat ggaactgaaa gcgacaacgc ttggaaaacg tctggcacag
     4081 cacccttacg atcgggcggt gatcctcaat gccgggatta aagtctccgg cgatcgccac
     4141 gaatacctta ttcctttcaa tcaattactg gcgattcact gtaagcgcgg tctggtatgg
     4201 ggcgagctgg agtttgtact gccggacgaa aaagtggtgc gtctgcacgg caccgaatgg
     4261 ggcgagacgc agcgttttta ccatcatctt gatgctcact ggcggcggtg gagtggcgag
     4321 atgagcgaaa ttgcgtctgg tgttttacgc cagcaactgg atttgattgc cacgcgcact
     4381 ggagaaaata aatggctgac gcgtgagcaa acctctggcg ttcagcaaca aatccgccag
     4441 gctttgtcgg cgttgccgtt gccggttaat cgactggaag aattcgataa ctgccgtgag
     4501 gcgtggcgta aatgtcaggc ctggttgaaa gatattgaaa gcgctcggtt gcagcataac
     4561 caggcgtata ccgaagccat gcttaccgag tatgcggatt ttttccgcca ggtcgagtct
     4621 tcaccgctga atccggcgca ggcccgggca gtcgttaatg gcgagcattc tctgttagtg
     4681 ctggcaggtg caggaagcgg aaaaacgtcg gtgctggtgg cccgtgcagg ctggttgctg
     4741 gcgcgtggtg aagcgtcccc tgagcaaatt ttattactgg cgtttggtcg caaagccgct
     4801 gaagagatgg acgagcgtat tcgcgaacgg ctacataccg aagacattac cgcacgcacg
     4861 tttcatgcgc tggcgctgca tattattcag cagggcagca aaaaagttcc gatagtcagc
     4921 aaactggaaa atgataccgc tgcccgtcat gaactcttta ttgctgagtg gcgcaagcaa
     4981 tgcagcgaaa agaaagcgca ggcgaagggc tggcggcaat ggctgacgga agaaatgcag
     5041 tggtcagtgc cagaaggtaa cttctgggat gatgaaaaat tacagcatcg tcttgcctca
     5101 cgcctcgatc gttgggtaag tctgatgcgg atgcacggtg gtgcacaggc agaaatgatt
     5161 gccagtgcac ccgaagagat tcgcgatctg ttcagtaaac gtatcaagtt gatggctcca
     5221 ttattaaaag cctggaaagg tgcgctgaag gcagaaaacg ctgtcgattt ttcgggcctt
     5281 attcaccagg cgattgtgat tctggagaaa ggtcgcttta tcagcccgtg gaagcatatt
     5341 ctggttgatg aatttcagga tatctcgccg cagcgggcag cgttgttagc ggcattacgc
     5401 aaacaaaaca gtcagacgac gttgttcgcc gttggtgatg actggcaggc gatttaccga
     5461 ttcagcggtg cgcaaatgtc gctcaccacc gctttccatg aaaactttgg tgaaggagat
     5521 cgctgtgatt tagacacgac ttaccgtttt aacagtcgta tcggtgaggt ggcaaaccgg
     5581 tttattcagc agaacccagg ccagctgaaa aagccgctaa acagcttaac caatggagac
     5641 aaaaaagccg tcacgttatt ggatgagagt caacttgacg ctttgctgga taagctctct
     5701 ggttatgcca aaccggaaga gcgcattctg atcctggcgc gttaccatca catgaggcct
     5761 gccagcctgg aaaaagcggc aacacgctgg ccgaagttgc aaatcgactt tatgaccatt
     5821 catgccagca aagggcaaca ggcggattac gtcctcatcg ttggcttgca ggagggaagt
     5881 gatggttttc cggctgcggc gcgggagtcg attatggaag aggcgctact gccaccggtt
     5941 gaggatttcc cggacgctga agaacggcgg ttaatgtacg tggcgctgac ccgggcacgc
     6001 catcgggtat gggcactgtt taacaaagag aatccctctc cctttgtgga aatactgaaa
     6061 aatctggatg tgccggtggc gagaaaaccg taagaaacag gtggcgtttg ccacctgtgc
     6121 aatattactt cagacggtcc gcgagataac gctgataatc ggggatcaga atatcgaccg
     6181 cgtcgttgaa atgcggcgac tggataatga agtctgccgt tgccacgttg gtggcgaccg
     6241 gaatgttcca taccgtcgcc agacgcagca aggctttcac gtcaggatcg tgcggcacgg
     6301 catttagtgg atcccagaag aaaatcaata catcaatttt cccttctgag atcaatgcgc
     6361 caacctgctg gtcacccccc attgggccac tcaacatcgc gttgacgttc atgccggtcg
     6421 cgcgggaaat taagttaccg gtagtgcctg ttgcatacag tacgtgttgt tccagtaacg
     6481 gttgatgccg ttccacccag ctcatcagca tttgtttgca gtgatcgtgt gccaccagcg
     6541 caatatgttt ccgcgcaggt aaagtgcgag tcgtcagttc cataatgtac atccgtagtt
     6601 aactttccta aagattactg taagcactta tcgctgcaag ataaagaccg aaaaagcctg
     6661 cgcacaggca caaaaatctc aggaagatgg ttgtttttcc gcccattgca ggaaagtatt
     6721 tcgcgtttgt gggtcagcca gtttaaacca atacttcagc cgttgttctg tgagcacctg
     6781 agactgcggt ggaatagcat ccagctcgga aacacccgaa agcagcgtac tgtcatctgc
     6841 catcatcgtg gcaaattgcg gtagtgaagc gcgtttcgcg gatttgttat agcgttcaac
     6901 ctcgacctca taatcaatcg tttttgccgt tgaggtaatg gcgagaatat ccagctttac
     6961 cggaatcggc gtcgcatcgc catccaacaa ttcaaggcgc ggcgcggcat caaaatggtt
     7021 agcttcccgc tcattctcca ggcgaggcag gcgaaaattg acctggttga tgagctgggt
     7081 attaaaactg accaccagcg gtggggagat atacagccgt tcttcactat tggaaagatg
     7141 aatcgtcttc tcaacgcgaa acactaactg atgcggtcca ttatccagtt caatgctgtc
     7201 cgcaccgcgc agcagagagc tggaaacctt cttgccgtcg agtaccagaa gatcgacatc
     7261 ggttgaaagc cgcagcgtag tggcaaaaac ggataccggc aaacataatg caatcaaggt
     7321 ggtcacgatg ccggttttca taggtagctc ctgtcaaaag accgcccgaa taataatgtg
     7381 tcaaaatttt tctttaatca tacttactaa catatagaca tatttcccgc ttttgctctc
     7441 attcattcgt attagctgca tggttggcat gtcggctttg tcgtacactt tgttaaacag
     7501 tctggaggaa ataatgaaag aaaccgatat tgcaggcatt ttaacgtcca ctcacaccat
     7561 tgcgctggtg ggggcaagcg acaaacccga tcgtcccagc tatcgcgtga tgaaatatct
     7621 actcgaccag gggtatcacg tcattccggt ttcgccaaaa gttgccggca aaacgctgct
     7681 gggccagcag gggtacggca cgctggcaga cgttccggaa aaagtcgata tggtcgatgt
     7741 tttccgtaat tcagaagcgg cgtggggcgt agcgcaggaa gctatcgcca ttggcgcgaa
     7801 aaccctctgg atgcaactgg gggtgattaa tgagcaagcg gcagtactgg cacgggatgc
     7861 cgggttgaat gtggtgatgg atcgctgccc ggctattgag atccctcgcc tgggcctggc
     7921 caaataaaaa atccccggaa ggcaaaaacc ttccggggat ttgttcaggg attagttacg
     7981 cagacgcggg gcctggagtt gtttgcggat ggtctgcgcc agctcatcca tggatggctg
     8041 ttcaggatgt tcatcctgca attcgctact tagctgggct tcagccaggt aggtatgaac
     8101 cggtaggccg ttatcgtcct ccatcaccac gtgataccac ggagcggcgc gaagctcgtc
     8161 attcaccgcc agctcatcag gcgacggttc agagagcgaa taaaccgggt cgatatccac
     8221 gaccactccg agataaccta acagggaatg gcggacctgc tggccgatac cgaatttgct
     8281 ggcaatcata gtcacctccc gggaaatctt tacctttgat ataagggtaa aattccacat
     8341 ttcaagttac atgacgcgac aggcaaaccc tttcagatat agcccttccg gataggtagc
     8401 gatcaccgga tgatcggccg cctgacggaa ctgctctata aattgtacat cacgaccggc
     8461 atcaattgcg gcatccgcga tgattttctg aaataaatcg ctggtcatca gaccggaaca
     8521 ggagaaagtc aggagaatac cgccttcatt cagcaactga atcgccagca tgttgatgtc
     8581 tttatagcca cgacacgcgc ccatcaactg gcttttattc tcaacaaact tcggcgggtc
     8641 catcacgata acgtcaaatt tttcaccgcg atcgcgataa gtacgcagca atttaaagac
     8701 atcatcacgg acaaactcag ccttgctcag atccagtttg ttcagctcaa cgttctgccg
     8761 tgcaatatcc agcgcttcct gggaggtatc aacgctgaca acctggttgc aaccgcccat
     8821 cagtgccgat acggcgaaac caccggtata ggagaaacag ttcagtacgc gtttattttc
     8881 aacgtagcgg cgggtagcca ggcggctgtc tcgctggtcc aggtagtagc ccgttttgtg
     8941 tccgtgctgt atgtccacca gcagcttcat gccatgttct tcaatcggca gcagggcagg
     9001 cggcaactcg ccggtgacgg ggccctgggt cagctccatc ccttcttttt tacgtaccgc
     9061 gacgtcgctg cgatcgtaaa tcgcacattc tgggtacagc gtttgcaggg cactaattaa
     9121 tgccgcgcgc tgatattctg cgccagcact cagcagttgc agcaccagaa aattaccgaa
     9181 acgatcgata gtaatacccg gcaggccatc agattcaccg gcgattaaac gatagctgtc
     9241 gaggccatct ttttgcgcca gccagtcacg ccatttttgt gcttgttgca aacggcggga
     9301 aaaaaaagca atgtcgatag actcagacgg gtcaaacgtc cagacgcgcg cccggatttg
     9361 cgaagctggc gaataagcgc cgcgtgctaa ccattttccc tgatgatcaa caatatcgat
     9421 ggtttcaccg aggctggctt taccttccat gcgggcaacg gccccggaaa agacccacgg
     9481 atggcgacga agtaatgatt tttcgcgccc tttggctaac actaaacgta cactcataat
     9541 ttacttttct gtcgatgcaa agaaatgggc gtcattgtcc ggagttttcc atggatttgc
     9601 aacgcgtcca ttaaggataa ggagagcagc aatgtcgaaa gtctgcataa ttgcctgggt
     9661 ttacgggcga gttcagggcg taggatttcg ctacaccaca cagtacgaag cgaaaagact
     9721 gggtttaacc gggtacgcca aaaatcttga cgatggcagc gtggaagtgg ttgcctgcgg
     9781 tgaagaaggg caggtagaaa aattaatgca gtggttaaag agtggcggcc cgcgttctgc
     9841 gcgtgtagaa cgggtgctta gcgagccgca tcatccctcg ggggaattaa ccgattttcg
     9901 tatccgttaa atacatttta ccggttttgg caggccagca attttggtgg cttgctttgc
     9961 cgggcctttc gggaacagtc ggtataaata gcggctgtta cctttttctt caccaaattt
    10021 attcgccatc gcttttacca gcatacgaat cgccggagac gtattaaatt ccaggtagaa
    10081 gtcacgcaca aaacgcacca cttcccagtg ttctggcgac agcgaaatcc cttcgttctc
    10141 tgcaatcacc actgccagcg gctcactcca ctggctgctt tctttgagat agccttcggt
    10201 atccgtttct atctctttac cttcgaagat cagcataatt attactacct taatcagact
    10261 gccggcagtg taacaaaaaa caaagccccg cataaagcga ggctatgaaa gtgttagcgg
    10321 gtgagattaa tcgcggctgg cgaagcccag aatgctcagc aggctgacga agatgttgta
    10381 cagcgaaaca tacaggctaa ccgtggcacg aatatagttc gtttcaccgc catgaatgat
    10441 gttgctggtt tcaaacaaaa tagcgccaga ggagatcaaa atgaagaccg cgctgatcgc
    10501 cagatgcaga gcaggcagtt gcaggaagat attagcaacc ataccaatca gcaccaccac
    10561 aatacccgcc atcagcatac cgccgaggaa cgacatatct ttgcgggtgg tcagcacata
    10621 tgcagagcag cagaagaaca ctaacgccgt tccgcccagt gccatagcga ttacgtcacc
    10681 cattccggca gacagatagg tgttcagaat tggtccgagg atataaccca gaaaaccggt
    10741 aaaggcgaat gcggagataa tcccggtcgg tttattcgcc gttttatagg tcaggaacat
    10801 caaaccatac atacccacca gcgtcagaat cagacccgga gatggcagca tcagcaccgt
    10861 actggcagtt gcggtaatcg ccgaaaaggc cagcgtcagg ctcagcagaa aataggtatt
    10921 acgcagcacc ttatgggtgc taagcagtga tgtacggtca tgtgaagaac taacaatacg
    10981 atccattgag tcactctcta tgacagatgt aattaattaa gcagcataat gataatgcgt
    11041 aagggcaccc agaagtttta cccatcttta cgcatttgat ctggaacagg tttaacagcg
    11101 gattatcagg tcattaagca aatataacgc cctgagaatt tcgacaggca aaagaaaaag
    11161 gggttagcat ttagctaacc ccttatctta tctggcggaa gcgcagagat tcgaactctg
    11221 gaaccctttc gggtcgccgg ttttcaagac cggtgccttc aaccgctcgg ccacacttcc
    11281 ggaatgacgc gcactataaa catcccgatg cggcgtgtaa acccctaatt tgtttgtttg
    11341 cctgaaaaac agccaaaagt gcattgatag cgtgaaataa cagcagattg atcatttcat
    11401 caccatgaat tccttctctt ttactcgttt agcaaccggc taaacatccc accgcccggc
    11461 aaaaagaaaa ataggtccat ttttatcgct aaaagataaa tccacacagt ttgtattgtt
    11521 ttgtgcaaaa gtttcactac gctttattaa cattactttc tggcgacgtg cgccagtgca
    11581 gaaggatgag ctttcgtttt cagcatctca cgtgaagcga tggtttgcct tgctacaggg
    11641 acgtcgcttg ccgaccataa gcgcccggtg tcctgccggt gtcgcaagga gaagagacgt
    11701 gcgatatgaa taacgaggaa acattttacc aggccatgcg gcgtcagggc gttacccggc
    11761 gcagctttct caaatattgt agtctggctg ccacgtcgct gggattaggc gcgggaatgg
    11821 caccaaagat tgcctgggcg ctggagaaca aaccgcgcat tccggtggta tggatccacg
    11881 gtctggaatg cacctgctgt accgaatctt ttatccgctc cgctcaccca ctggcgaagg
    11941 acgtcatcct ttccctgatt tccctcgatt atgacgatac tttgatggct gccgccggaa
    12001 cccaggcgga agaagtcttt gaagacatca tcacgcaata caatggcaaa tatatcctcg
    12061 cagtagaagg taatccgccg ctgggcgagc aggggatgtt ctgtatcagc agcggtcgac
    12121 cgtttattga gaaactcaaa cgtgccgctg ccggagccag cgcgattatc gcctggggaa
    12181 cctgcgcgtc ctggggctgc gtgcaggccg cgcgacctaa tccgacacag gcaacgccta
    12241 tcgacaaagt catcaccgac aaacccatta tcaaagtacc tggctgcccg ccgatcccgg
    12301 atgtgatgag cgccatcatt acttacatgg tgacctttga tcgcttgcca gatgtcgaca
    12361 gaatgggccg tccgttgatg ttctatggtc agcgaatcca cgataaatgc tatcgccgcg
    12421 cccacttcga cgccggagag ttcgtgcaga gttgggatga tgacgctgcc cgcaaaggtt
    12481 actgcctgta caaaatgggc tgcaaagggc ctaccaccta taacgcctgt tcctccacac
    12541 gctggaatga tggcgtttct ttcccaatcc agtctggtca cggctgcctg ggctgtgcgg
    12601 aaaatggttt ctgggatcgc ggttcgttct acagccgcgt ggtcgatatt ccacaaatgg
    12661 gtactcattc caccgccgat accgtcggtt taaccgcgct tggtgtggtg gcagcggctg
    12721 ttggtgtgca cgcagtcgcc agcgccgttg accagcgcag acgtcataat cagcaaccta
    12781 cagaaaccga acatcagcca ggcaatgagg ataaacaggc atgagcactc agtacgaaac
    12841 tcagggatac accatcaata atgccggacg ccgcctggtg gtcgacccga ttacgcgcat
    12901 tgaaggccac atgcgctgcg aagtgaatat taacgatcag aatgtaatca ccaatgccgt
    12961 ctcctgcggc accatgtttc gcgggctgga gatcatcctg caagggcgcg acccgcgcga
    13021 tgcgtgggcg ttcgttgaac gtatctgcgg cgtctgtact ggcgtacacg ctctggcttc
    13081 ggtttacgcc atcgaagatg ccatcggtat taaagtgccg gacaacgcca atatcatccg
    13141 caacattatg ctggcaacgc tctggtgcca cgatcatctg gtgcacttct atcagcttgc
    13201 cgggatggac tggatcgatg tgttggatgc gctgaaagcc gacccgcgga aaacctccga
    13261 actggcgcaa agtctctcct cttggccgaa atcatcccct ggctatttct tcgacgtaca
    13321 aaatcgcctg aagaaattcg ttgaaggcgg ccagttgggg atcttccgca atggctactg
    13381 ggggcacccg cagtacaaac tgccgccaga agccaacctg atgggctttg cccactatct
    13441 cgaagctctc gatttccagc gtgaaattgt caaaatccac gcggtctttg gcggtaaaaa
    13501 cccgcatcca aactggattg tcggcgggat gccttgcgcc attaacattg acgaaagcgg
    13561 cgcggtcggg gcggtcaata tggaacgcct gaacctggtg cagtcgatta tcacccgcac
    13621 ggcagacttc attaacaacg tgatgatccc cgacgcctta gccattggtc agttcaacaa
    13681 accgtggagc gaaatcggca cgggtctttc tgataaatgt gtcctcagct acggcgcatt
    13741 cccggatatt gccaacgact ttggcgagaa aagtctgctg atgcctggcg gcgcggtgat
    13801 taacggcgac ttcaacaatg tgctgccagt ggatttggtt gatccgcagc aggtccagga
    13861 gtttgtcgac cacgcctggt atcgatatcc caacgatcag gtcgggcgtc atccattcga
    13921 tggcattacc gacccgtggt acaaccccgg cgatgtcaaa ggcagcgata ccaacattca
    13981 gcagctgaat gaacaggaac gctactcgtg gatcaaagcg ccgcgctggc gcggtaacgc
    14041 gatggaagtg gggccgctgg cacgcacgtt aatcgcttat cacaaaggcg atgctgcgac
    14101 cgttgagtcg gtcgatcgca tgatgtcggc gctgaacctg ccgctttccg gtattcagtc
    14161 aacgttgggc cgcattttgt gccgcgcgca cgaagcacag tgggccgcag gtaagttgca
    14221 gtatttcttc aacaagctga tgaccaatct caaaaacggc aatcttgcca ccgcctccac
    14281 ggaaaaatgg gaacccacaa cctggccgac agagtgccgt ggtgtcggct ttaccgaagc
    14341 gccgcgcggg gcgttaggcc actgggccgc cattcgcgat ggcaagattg atctctacca
    14401 gtgcgtggtg ccgaccacct ggaacgccag cccgcgcgat cccaaagggc agattggcgc
    14461 ttatgaagcg gcgctgatga acaccaaaat ggcgatcccc gagcaaccgc tggagatcct
    14521 gcgtactctg cacagctttg acccgtgcct cgcctgttca acacacgtgc tgggcgacga
    14581 cggtagcgag ctgatctccg tgcaggtgcg ttaacagcga aggagaatca tcatgcaaca
    14641 gaaaagcgac aacgttgtca gccactatgt ctttgaagcg ccagtccgca tctggcactg
    14701 gctgacggtg ttatgcatgg cggtgttgat ggtcaccgga tactttatcg gcaagccgct
    14761 accttccgtc agcggcgagg cgacgtatct gttctatatg ggctacatca ggttaattca
    14821 cttcagcgcc gggatggttt ttaccgtggt cttgctgatg cggatctact gggcttttgt
    14881 tggcaatcga tactcccgcg agctgtttat cgtgccggta tggcgtaaaa gctggtggca
    14941 gggcgtgtgg tatgaaatcc gctggtatct gtttctggca aaacgtccga gtgccgatat
    15001 aggccataac cccatcgccc aggcggcgat gttcggctat ttcctgatgt cggtctttat
    15061 gatcatcacc ggctttgcgc tgtacagcga acacagccag tacgctattt ttgcgccgtt
    15121 ccgttatgtg gtggaatttt tctactggac gggaggcaac tcaatggaca ttcacagctg
    15181 gcatcggctg gggatgtggc tgattggcgc gtttgtgatc ggtcatgtct atatggcgct
    15241 gcgtgaagac atcatgtcgg acgacacggt gatctccacc atggtcaacg gctaccgtag
    15301 ccacaaattt ggcaaaataa gtaacaagga gcgttcatga gcgagcaacg cgtggtggta
    15361 atggggctgg gcaacctgct gtgggccgat gaaggtttcg gcgtgcgggt ggcggaacgg
    15421 ttgtatgccc attaccactg gcccgagtat gtggagattg tcgatggcgg tactcaggga
    15481 ctgaacttgc tggggtatgt cgaaagcgcc agccatctgt tgattctcga tgccattgac
    15541 tacgggctgg aacctggaac gctgcgaacc tatgccggag aacgtattcc ggcttatctc
    15601 agcgcgaaga aaatgagcct gcaccagaac agtttctccg aagtgctggc gctggcagat
    15661 atccgcggac atctgccagc acatattgcc ctcgtcggtc tgcaacccgc aatgctcgac
    15721 gactacggcg gcagcctgag cgaactggca cgggagcaac tgcccgcagc ggaacaggcg
    15781 gcgctggcgc agctggctgc atggggaatt gtgccgcaac cggctaatga atcgcgctgt
    15841 ctcaattatg actgtctgtc gatggaaaat tacgaaggcg ttcgcttgcg ccagtacagg
    15901 atgacactgg aggagcaggg atgagcaacg acacgccatt tgatgcgttg tggcaacgaa
    15961 tgctggcgcg cggctggacg ccggtcagtg aatcccgtct tgacgactgg cttacgcaag
    16021 cgccagacgg cgtggtgtta ttaagcagtg acccgaaacg cacgccagag gtcagcgata
    16081 atccggtaat gattggcgaa ttactgcgcg agtttcccga ctatacatgg caggtggcga
    16141 ttgctgacct tgagcagagc gaagccatcg gcgatcgctt tggcgtcttt cgctttcccg
    16201 ccactttagt gtttaccggc ggaaactatc gcggcgtgct gaatggtatt cacccgtggg
    16261 cggaactgat aaacctgatg cgtgggcttg tcgaaccaca acaggagcgt gcctcatgag
    16321 cgaaactttt ttccatctgc tggggccagg aacgcaaccg aacgatgaca gtttcagcat
    16381 gaatccactg ccgatcacct gtcaggtgaa tgatgaaccg agtatggcgg ccctggagca
    16441 atgtgctcac agcccgcagg tgaatgcgct gttaaacgag ttacaacatc aactaagtca
    16501 acgccagccg ccgttgggcg aggtgctggc agtcgatctg ttaaatctca acgccgacga
    16561 tcgtcacttt atcaatacgc ttctcgggga aggggaagtg tcagtgcgca ttcagcaggc
    16621 tgacgacagt gaaagtgaaa tacaggaggc gatcttctgc ggattatggc gggtgcgcag
    16681 acgtcgcggc gaaaagttgc tggaggacaa actggaagct ggctgcgcgc cgctggcgtt
    16741 gtggcaggcg gcaacgcaaa atctcttgcc gacagattcg ctgttaccgc cgcccattga
    16801 tggcctgatg aatggcctac cgttggcgca tgagttactg gcgcatgtac gtaaccccga
    16861 cgcgcagccg cacagcatta atctgacgca attacccatc agcgaggctg atcggctttt
    16921 tctctcacgt ctctgtgggc cgggaaatat tcagattcgt accattggct atggcgagag
    16981 ctatatcaac gccacggggt tacgccatgt ctggcattta cgctgtacgg acaccttaaa
    17041 aggcccgtta ctggaaagtt atgaaatctg cccaataccg gaagtggtgc tggcagcgcc
    17101 agaagatttg gtcgactctg cgcagcggct tagcgaggta tgtcagtggc tggcggaagc
    17161 tgcaccgacg taaaaagacg gtaagtatcg ctttcaggct tatgaatatc gcaatcggcg
    17221 aatacctctg gtcgtagagt ttcaggataa agagggagat ctaccattaa cgggttattt
    17281 ttctctcttc gcctacagga gtgcgcatgt gggatgtcat tgatttatcg cgctggcagt
    17341 ttgctctgac cgcgctgtat cactttttat ttgtacccct taccctgggg ctgatttttt
    17401 tgctggctat catggaaacc atttacgtgg tcaccggcaa aacaatctac cgcgatatga
    17461 cgcgcttctg gggtaagctc ttcggtatca actttgctct tggcgtggct accggcctga
    17521 ccatggagtt tcagtttggt actaactggt cattttattc caactatgtg ggcgatattt
    17581 ttggcgcacc gctggcgatg gaagcattaa tggccttctt cctcgaatcc acctttgtcg
    17641 ggcttttctt cttcggctgg cagcggctga ataaatacca gcacctgctg gtgacgtggc
    17701 tggtggcgtt cggttcaaat ctctctgcat tgtggatatt gaatgccaac ggttggatgc
    17761 aatacccgac cggtgcgcat tttgatatcg acaccctgcg tatggagatg accagcttca
    17821 gcgagctggt ctttaacccg gtcagccagg tgaaatttgt gcacaccgta atggcgggct
    17881 acgtaaccgg ggccatgttt attatggcga tcagcgcctg gtatttactg cgcggacggg
    17941 agcgcgatgt cgcattacgc tcgtttgcca tcggttccgt cttcggtact ctggcgatta
    18001 tcggtacgct gcaacttgga gacagttctg cgtacgaagt cgcgcaagta cagccggtaa
    18061 aactggcggc gatggaaggg gagtggcaaa cggaacctgc acctgcaccg ttccatgtgg
    18121 ttgcctggcc ggaacaggat caagagcgta acgcctttgc tatcaaaatt cccgcgctgc
    18181 tagggatcct cgccactcac tcattagata aacccgtgcc gggtctgaag aatttgatgg
    18241 ctgaaaccta cccacgcttg caacgcggac gtatggcctg gctgttaatg caggaaatat
    18301 cgcaaggcaa tcgtgagccg catgtgttgc aggcattccg ggaactggaa ggtgacctgg
    18361 gctacggcat gttgctctcc cgctatgcgc cggatatgaa tcatgtcaca gccgcacagt
    18421 accaggcggc gatgcgtggt gcaatacctc aggttgcgcc ggtattctgg agtttccgca
    18481 tcatggtggg ctgtggttcc ctgctgctac tggtgatgct gattgcgctt gtccagacgc
    18541 tgcgtggcaa aatcgaccag catcgctggg tgctgaaaat ggcgctctgg agtttgccgt
    18601 tgccgtggat tgcgattgaa gccgggtggt ttatgaccga gtttggtcgt cagccgtggg
    18661 cgatacagga catcttaccg acatactccg cgcactccgc cttaactaca ggacaactgg
    18721 ctttctcact gatcatgatc gtagggcttt acaccctgtt cttaatcgcc gaagtctacc
    18781 tgatgcagaa atatgcccgt ctggggccga gcgcgatgca gagtgaacaa ccgacgcagc
    18841 aacaggggta aaggagaaaa tcatgtttga ttatgaaaca ttgcgcttca tctggtggct
    18901 gctgattggc gtgatcctgg tggtctttat gatctccgac ggatttgaca tggggattgg
    18961 ctgtttgctg ccgctggtgg cgcgtgatga tgatgaacgc cggatagtga ttaatagcgt
    19021 tggtgcgcac tgggaaggca atcaggtctg gttgatcctc gcaggtgggg cattatttgc
    19081 cgcctggccc agagtgtatg cagcggcgtt ttccggcttt tatgtggcga tgatcctggt
    19141 gctgtgctcg ctgttcttcc gcccgctggc ctttgattat cgcggaaaaa tcgccgatgc
    19201 acgctggcgt aaaatgtggg atgccggtct ggtcatcggc agtctggtgc cgccggtagt
    19261 cttcggtatc gccttcggca acttgttgct cggcgtgccg tttgccttca caccgcaatt
    19321 acgcgtggag tatctcggca gcttctggca actgctgacg ccattccctt tattgtgcgg
    19381 attgctcagc cttgggatgg tgattttgca aggtggcgtc tggttacaac tgaaaactgt
    19441 tggtgtgatt catctgcgtt cacagctggc gactaaacgc gctgcactgt tggtgatgct
    19501 gtgctttttg ctggcgggtt actggctgtg ggtcggtatt gatggctttg tactgctcgc
    19561 ccaggatgct aacggtcctt ccaatccgtt aatgaaactg gtggcagtgc tacctggtgc
    19621 ctggatgaat aattttgtcg agtcgcccgt tttgtggatc ttcccgctgc tggggttctt
    19681 ctgcccattg ctgacggtga tggcgattta tcgtggtcgc ccgggttggg gatttttgat
    19741 ggcgtcattg atgcaatttg gcgtgatttt cacggcaggc atcacgctgt tcccctttgt
    19801 catgccgtca agcgtgagtc caatctccag cctgacgttg tgggacagca cttccagtca
    19861 gctgacgctg agcattatgt tggtaatcgt gctgatattt ttgcccattg tgttgctcta
    19921 cactctctgg agctattaca aaatgtgggg gcgcatgacg acagaaactc tccgccgtaa
    19981 cgaaaacgag ctgtactaag gagcaggaac aatgtggtat ttactttggt tcgtcggcat
    20041 tttgttgatg tgttcgctct ccacccttgt gttggtatgg ctggacccgc gattgaaaag
    20101 ttaacgaacg taagtctgat gcggcgcatt agcgtcgcat caggcaatcc ataatgtcgg
    20161 atatgaaaag cggaaacata tcgatgaaag cgatcttgat cccattttta tctcttctga
    20221 ttccgttaac cccgcaatct acattcgctc agagtgagcc ggaactgaag ctggaaagtg
    20281 tggtgattgt cagtcgtcat ggtgtgcgtg caccaaccaa ggccacgcaa ctgatgcagg
    20341 atgtcacccc agacgcatgg ccaacctggc cggtaaaact gggttggctg acaccgcgcg
    20401 gtggtgagct aatcgcctat ctcggacatt accaacgcca gcgtctggta gccgacggat
    20461 tgctgacgaa aaagggctgc ccgcagcctg gtcaggtcgc gattattgct gatgtcgacg
    20521 agcgtacccg taaaacaggc gaagccttcg ccgccgggct ggcacctgac tgtgcaataa
    20581 ccgtacatac ccaggcagat acgtccagtc ccgatccgtt atttaatcct ctaaaaactg
    20641 gcgtttgcca actggataac gcgaacgtga ctgacgcgat cctcagcagg gcaggagggt
    20701 caattgctga ctttaccggg catcggcaaa cggcgtttcg cgaactggaa cgggtgctta
    20761 atttcccgca atcaaacttg tgccttaacc gtgagaaaca ggacgaaagt tgttcattaa
    20821 cgcaggcatt accatcggaa ctcaaggtga gcgccgacaa tgtctcatta accggtgcgg
    20881 taagcctcgc atcaatgctg acggaaatat ttctcctgca acaagcacag ggaatgccgg
    20941 agccggggtg gggaaggatc actgattcac accagtggaa cacgttgcta agtttgcata
    21001 acgcgcaatt ttatttacta caacgcacgc cagaggtttc ccgcagccgc gccaccccgt
    21061 tgttggattt gatcatgaca gcgttgacgc cccatccacc gcaaaaacag gcgtatggtg
    21121 tgacattacc cacttcagtg ctgtttatcg ccggacacga tactaatctg gcaaatctcg
    21181 gcggcgcact ggagctcaac tggacgcttc ccggtcagcc ggataacacg ccgccaggtg
    21241 gtgaactggt gtttgaacgc tggcgtcggc taagcgataa cagccagtgg attcaggttt
    21301 cgctggtctt ccagacttta cagcagatgc gtgataaaac gccgctgtca ttaaatacgc
    21361 cgcccggaga ggtgaaactg accctggcag gatgtgaaga gcgaaatgcg cagggcatgt
    21421 gttcgttggc cggttttacg caaatcgtga atgaagcacg cataccggcg tgcagtttgt
    21481 aatgcataaa aaagagcatt cagctacctg aatgctctga ggctgatgac aaacgaagaa
    21541 ctgtctaatg cgtagaccgg aaaaggcgtt cacgccgcat ccggtcactt taccttcagt
    21601 tttactcttt ctcggagtaa ctataaccgt aatagttata gccgtaactg taagcggtgc
    21661 tggcgcgttt aatcacacca ttgaggattg cgcctttaat attgacgcct gcctgttcca
    21721 gacgctgcat tgacaagctc acctctttgg cggtgttcaa gccaaaacgc gcaaccagca
    21781 ggctggtgcc gacagagcgc cccacgaccg cagcatcgct caccgccagc atcggcggcg
    21841 tatcaacaat caccagatcg taatggtcgt tcgcccattc cagtaattga cgcatccgat
    21901 cgcgcatcag cagttcagac gggttaggtg gcacctgacc gcgagtaatc acatcaaagc
    21961 ttcctttgcc aaaatgctgg atcactttgt tgagctcatc tttacctgcc agatattccg
    22021 acaagccatg ttcattactc acggtaaaca ggttatgcga ataaccacgg cgtaagtcgg
    22081 catcaataaa taacactttt tgatcggact gggcgatcac cgctgccaga gttgaactga
    22141 caaatgtttt accactgtct ggcgtcgcac cggtgatcat cagaatgtta ttctcagtct
    22201 ccatcatagc gaaatgcaga ctggttcgta gcgcacgtac ggcttccaca gcagaatccg
    22261 ccgggttatc caccgccagg aaggggatat ttttagtacg atggcgctgc tgattagaaa
    22321 ataaattttt cttacgcaga cgggtacgtt tatccagcca ctcggacatc gggatggtgg
    22381 cataaacgct gatgccgtgc tcttccagtt gttccggggc ttctacacca cgacgcaaca
    22441 tcgcacgcgc cagcacggca cccaccgaaa taaacaggcc aagaataaaa ccaagcacca
    22501 cattcaacgc ttttttcggt ttcactggct gcggctgagt gactgccggg tcgataatcc
    22561 gcacgttacc aatcgcactg gatttcgaaa tactcaactc ctgctggcgg ttaagtaatt
    22621 gcagatatac cgcacggccc gcttctacgt cacgacttaa acgcaacact tcctgttggg
    22681 tggaaggcat tgccgatacc cgcttattca ggcgtttgcg ttcttgctcc agcgtctggc
    22741 gtttttccag cagcgcacga taagttgggt gatctttctt atacagctgg gagatctctg
    22801 cctcgcggaa ggtcagctca ttgagttgat tatcaacgtt cacaatctgc tcaagaacgg
    22861 ctttggcttc caggttaagg tcaaccgaat cgcgctgctg gcgataaacg ttgagttttt
    22921 cttccgcctg gtccagctcg ctgcgcactt caggcaactg gcgctgtaag aattcaaggc
    22981 tttgtgaatc ctgcgccgcc tggcgagcga tattctgttg caaatagttg ttagcgatgc
    23041 tgttcagtat acgggtaatc aactggggat catcaccggt catggtaagt tccagcatcc
    23101 cgctttcttt actgcgttcg ctaacggtaa aggtttcctg caacgcgtta atcgcttcca
    23161 gttcggtacg ttggctcagg acaaactgtg ttcctggttt ggccttaata tccgcgatag
    23221 tcagcgcaac gccatctttt tccagacgct ggccgaccat accattgacg gtgaactctt
    23281 caccttccag cgtatagtgg ccgttttccc caaccgtgag tgtcagttgc tgatcctgac
    23341 cattcagttg tggaatatgc atccagctga tcgccagctc acctggtttc tctttggtta
    23401 atcttgccca gccgcgaccc acaatcggaa aatacttctg ctcaaccatg tcgcgcagat
    23461 tcagttcagc aatggtttta ccgagaatca tgcgcgattg cagcagttgg atctccggtg
    23521 cagactcggg cgatgagtta gggatcatat cgctcaggcc gctgagaatg gcgttgccct
    23581 gtttctgctc aacctggacc agagtatctg cctgataaat tggcgtgctt aacagcgagt
    23641 aagcgacagc gatcagggtg aataacgcgg tcacgctgat aataaactta cggtgatccc
    23701 ataactcgcc gaccagacga agcagatcga tctcattttc ctgagtgctg cctggtggcg
    23761 tattcatatt tttagttgtc atcgtatttt ataaactcaa attaccggct gaggcgcttc
    23821 gcccattcct gactggcgcg ctccaacata ccgtagacat gttcaaatgc gtcctgactt
    23881 ttacgatagg gatccgggat ctctttctgt tccagccatt gcccaaacag cattgtcttc
    23941 ccgcggacct cgggagcgat tgcggtaacc tgagcaatat gttccgactc cattgccaga
    24001 atcagatcgt aatttcgtgc catctcagca gtgagcttac gtccggcatg tccttcaaga
    24061 gatacgccat gattagcggc aacatctgcc gctgtcgcat ctgcagggtg ttttaccaga
    24121 ccatgaacac ctgccgattt gacttttacg cccggcagac gtttacgcag caagcgctcg
    24181 ccaatgggcg aacggcaaat gttgccggta caaaccacca ggattgagtt aaattttagt
    24241 tgggccatgt ttgaatccac cgtgaagtct ctgtcaggtc atgcaccccg gaaatcgtcg
    24301 gtaccagcag ggaaatcaca cggttccaac gagccagcgg cgcggtagtg acataaacaa
    24361 tgtcgtaagg ttcaagctgg aattccgttc cgagtatcat tgccgatgcg tccttcgcat
    24421 tcagctgata aatattggcg attttgccat tctgcttgtt ttgcgttgca cgaataacaa
    24481 aaatccccgt cgcatcagcg acatcctgat tcataccctc agcgttaccc agcgcttccg
    24541 ccagggtcat accgctgcgg tccattttca gcgtgctctg tttacccact tcacccatca
    24601 cgaagacttt cagatcatcg ttgcgcggaa taaataaaat atcgcccggg tgcagcagtt
    24661 tgttctggcg caagtcgcca cgctgcatca gcgcatagag gttcactttg gtctttacgc
    24721 cattctgggt gagcaccacg ttgcgccagt ctgcatctgc ggtcagaccg cccgcggcgt
    24781 taatcgcatc catgatggtc agaggaatat tggtaatcgg ctgctgaccc gatttgctga
    24841 cttcgccggt gacgtacgct ttttgcgagc ggaacgccgc gacgctgaca tcgacttgcg
    24901 ggctttcaat gactgaatcc agacgcgctg taatctcgtt gcgtacctgg gtaagtgttt
    24961 tcccggccac tttcaggcga ccaatatagg ggtagaaaat cgcaccatcg gcgttaaccc
    25021 agttgccggt atcactggcg ctacggtatt gccctgcggg ggtggttagt tccgggtgat
    25081 cccagaccgt aaccatcaac acatcaccaa tgccgatacg atattcatag ttcgcgattt
    25141 gctgctccag ctccgggtta gcctgcgaca taatcggttt ggcgcgcagc tgatcaatca
    25201 atcctggcgt gacgggatag atattgacca ttttgtccag gtcatactga ttgtcaggta
    25261 gttcaatgac atccttgtta ctggtcgata aattctgacc aggaacaagg gtacatgctg
    25321 tcaaagacag aacagccaat gtcagtaccg aaaacttaaa aatgttcttt ttcattggtt
    25381 tatatttatc agtcaatgta atgttatgaa ttacgtcagg ttattgtcca accggtactt
    25441 ctcgctctgc cgtcattgga tagagctggt atttgcgtga cagcatttgt ccaccatcac
    25501 gcgtcaatgg tgtccacgaa accaccgcgc ggttgcggtt aggtccaatg gtcatcaagt
    25561 cgaacggaat cgagatataa aaacctttac taaagccgcc ttcgccgtag tcatctttag
    25621 atacgttact gattgccgcc cataccccta ccgccacgcc gctgtcaaag cgtttggcga
    25681 cgtcgatcgt tgcgccttta tcttttgcca gatattgccc aacgctaagt ttcatcagta
    25741 cgccattgag cgtcggcggg ttccagtaag ccgtcacgaa accagttggc gtggaataat
    25801 cggtgaagcg catcatgttg tcccagtcac gttgcttcac gtagttaacg tccacaccca
    25861 gcgcccagct ggcatctagc gggcgataaa gcagctcgga accgacaccg gcgtacatcg
    25921 tttccagata accgccatac acctggccat agaaaccatt gcctaagtcg gcaaagtagt
    25981 tcgcctgcaa gttgttgaga taaacgtcat tgcgaacgta atcacggata tgcgtgcgca
    26041 cgcggggcag ggtagagtcc gcgggtaata gcgaagactt aaacttgtcg tagttattgt
    26101 aaatattggt gaaaataccg ccatcaagca gcaggtggtc agtaaaccag tagcgggcgc
    26161 tggacatcag ccccagctgg aacatataga aatcttccgg gccgcccagc gattgtgaaa
    26221 gtgttgggtt gaaagagtag ctaaagcgat cttcacgaat acggtagccc cgaccaaagg
    26281 cagaaagatc ttctgcttca acacgttgtt gttgcaattg ctctgattta ccaggcgctg
    26341 taccagccag ctgtttgcgc aggctggcta cgtcggtttc ggtagtcacc atcgccatat
    26401 gctcgcgctt ttgcgtcacg ctaatcttct caacgccttg cggcaggtta ttcaccagaa
    26461 tccggttggc acgatcgacc gcttcacgag agtcacggta tttgtattgc tgaccagaca
    26521 tatacagcgt cttatcgcgt agctgaattt ctggcgcgtc aaagcccgcg ttatacttca
    26581 gggcggtaag ttggtttgct accgtggtgt actgcaatcc ttcagattca ggcgcaggtt
    26641 gatatgccgg ttttggcgta tcgcgcaggg cagggcgcag atcgttgaaa ttggtccgta
    26701 aagtgaaacc aaacatcaac gtgttaccgc gttcataact caggttgagg tctgcccagc
    26761 tggcagcgcg ataaactgca ccgacgttga aatggcttgc ctgaggcagt ttgccagcga
    26821 aatcattctg gtaattgttg ccgtcgtatt cgagtttcag acgcagggga ttccacggcg
    26881 tttgatactc aatgccgcca aagatggagg ccgggccacg aaagatatcg ctaaagctga
    26941 tatcgcccgc atcgtgagac tctgcgcgat gacaatattt atcgctcacc cggcaaaacg
    27001 ggttggtaat attgcccgca ttaccggcgt agccccatgc catcccgagg gtgaaatcaa
    27061 atggccccgc ttgcttgctg gccaccagat actcaccgtc aaacagaccc gtaccagcaa
    27121 tatcacgttt accaaacgcc acttgcggta gccagtaacc ttcttcccac aggcgaagtt
    27181 taaaatcgaa tgatttgtct ttgtatgact gatcgccgct gaaatcttcc aactggctat
    27241 atttgcgtgt gcgcacatcc gtataacgaa tggtgccttc cagccatggg aacagcgcca
    27301 cggacgtgga gtagaaacgg tattgatcgt tatcccggta gttgacgctg aattcaccct
    27361 ccggtgcgat gcgtgcattt ggcatctgca acaatcctgt gccgccaaag tccgattgcg
    27421 acgaacccag cggatccggg taggttaata cttcagcatg acaggcactg gagacggcaa
    27481 tggccaggca gcttaaaaga taagaattct tcttcatcag tcaggaacac gttgcgttaa
    27541 aacagacaca atctggtcat ttaaatcagc atatttttcg ggcagtacat gcgcagaaaa
    27601 gcctaaccaa agctggctgc caggcggtgg ttccacatgt cttttattcc acagggcaac
    27661 aggggcgacg acagtctcac cctcaggagt aatcaccata acgttattct tgtccgcgcc
    27721 tgcaagacgg gggtgatctt gcagataatc ggtaacgcta cggcctgcct gccagggcaa
    27781 ttgcccagcc ccagaaaccg cacccagcag agtaatagtc accggacgct gtacggtata
    27841 gagcgtatag tccccgacca gcgggggatt gctattctca tccactcgta caaaatcagg
    27901 gtccagtttg accggtaagc gaccagtgat attgagattg agtaactgct ggcgcacgga
    27961 tttaatcgtt gccgccacat cgtcgtcagc ttctgcttcc caggaagcaa gctgtgccat
    28021 gacatgttgg tagtctttaa gcgctttagc tttcaccgcg gagtcggtca gcagggcacc
    28081 aggccaccag aggcgatcgc gcagttgcgg ttgtgtcacc aactggacaa cattttccac
    28141 ggggccaacg gaaagtgtct gttgttcgcc aggcagataa atagtcaccg ttccttgcgc
    28201 aaaggcatgg ggtgtcatta cgtaaagtac gctggcaatg aaatacgact gtaatttatt
    28261 catggtgcgg gtttaagaaa cgtcatttct acgggaataa cccctgcgcc taacatttgc
    28321 cgactttgac gaacctggcc agtagccgaa tcgacccaga atgtgttttg ccactgtttt
    28381 tctggtagcg tggaagtcac ctcttcctgc cagacattac acagcacatc gcgacccgca
    28441 atatgcagcg tctctaatcc agcaaaggaa aaagtggaac tcagggtcgc agagcgaaaa
    28501 tggttgtctt cactccagag aatatcgcgc gtccagcggc tgccgtcttt tattgtcagc
    28561 gcgttacgca gagggtcctg cccggaattt gtcacttcca gcagattatt attaagtttg
    28621 acggtcttta aaagacgtcc attatgagta accagcatgg cgttatcctg ggataaccat
    28681 ttgctttgtt cttgttctat atatcccagt accacaaaga ttcgttgccc ttcattaagg
    28741 cgtaaataca tcgtggaata aggcaacgcc tggatctgct gatccgctac ggtgatatcc
    28801 tgattatcga aaaggctggc gcgaaatgta tcgaccatac tttgctggct gtgcgtacat
    28861 cccgcaagaa atagtgcgaa aatcgataaa ataagagggc gcacctttaa tccttgaaag
    28921 gttaattaaa aaaatgcaaa tctctaaagc ccggtacgaa tatcgcaacc gggtattatt
    28981 taactttaat ttcgacgcgt ttaagcagaa ttagcgcgta gtggacgtgg tggttgaggt
    29041 gttatgagaa ccgccatcgc cactgtcatt tgctgcaact actgccaacg tcgccacacc
    29101 aacagccgtt gccacaccga caactgtcgc cgtgccgaca gcgccagcag ccacgccacc
    29161 agtggtagca gccgtcgtgg ttgcaccggt agtggtcgtt gtggtgccag tagtacccgt
    29221 cgttgcctcg gtcccgtttg ctgcttcagg ggcagcaaac gcagctggcg tcgtcaacat
    29281 gaaggccata aggattgcag aaagcttgtg tttcataact tttcctttat tcatcgcatg
    29341 gacaatacgt acgcttgaga tcaggattgt ctactttgag tataacatcc ttgtgaaatc
    29401 tccctttcct cataccactt gcactatata cagcgggtga gaaaggcgcg cagagggaaa
    29461 tataagattg ttaactattc ccctctggaa atacgacttg tgagcataag ataaatctta
    29521 gcaaaataca aattaaagat aagaaaattg ttaagaaagc tattaactat tgtt
//
LOCUS       DAPGOF010000049        29097 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.049, whole genome shotgun sequence.
ACCESSION   DAPGOF010000049 DAPGOF010000000
VERSION     DAPGOF010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29097
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.049"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(143..1618)
                     /gene="ampG"
                     /locus_tag="RHV13_001855"
     CDS             complement(143..1618)
                     /gene="ampG"
                     /locus_tag="RHV13_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HDV1241394.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LFGGILMQRLSLFRALMIFGILQGASNAGYWLLSVTDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWPTFYLFSVAAA
                     IPGLILLLVCRQTLEYTRVNDNFIPRTEYPSGYAFAMWTLAAGFGLLAVWLLLLTMDA
                     LDLTHFSFLSALLEVGVLVALCGVVLGGLLDYLALRKTHLT"
     gene            complement(1662..2240)
                     /locus_tag="RHV13_001856"
     CDS             complement(1662..2240)
                     /locus_tag="RHV13_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDV1241395.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            2545..2862
                     /gene="bolA"
                     /locus_tag="RHV13_001857"
     CDS             2545..2862
                     /gene="bolA"
                     /locus_tag="RHV13_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HDV1241396.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            3206..4504
                     /gene="tig"
                     /locus_tag="RHV13_001858"
     CDS             3206..4504
                     /gene="tig"
                     /locus_tag="RHV13_001858"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDV1241397.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNQLKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            4750..5373
                     /gene="clpP"
                     /locus_tag="RHV13_001859"
     CDS             4750..5373
                     /gene="clpP"
                     /locus_tag="RHV13_001859"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDV1241398.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            5499..6773
                     /gene="clpX"
                     /locus_tag="RHV13_001860"
     CDS             5499..6773
                     /gene="clpX"
                     /locus_tag="RHV13_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HDV1241399.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            6961..9315
                     /gene="lon"
                     /locus_tag="RHV13_001861"
     CDS             6961..9315
                     /gene="lon"
                     /locus_tag="RHV13_001861"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HDV1241400.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            9524..9796
                     /gene="hupB"
                     /locus_tag="RHV13_001862"
     CDS             9524..9796
                     /gene="hupB"
                     /locus_tag="RHV13_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HDV1241401.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            9988..11859
                     /gene="ppiD"
                     /locus_tag="RHV13_001863"
     CDS             9988..11859
                     /gene="ppiD"
                     /locus_tag="RHV13_001863"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDV1241402.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDVAAKVKHEKALDAYYALQQKIS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            12010..12381
                     /locus_tag="RHV13_001864"
     CDS             12010..12381
                     /locus_tag="RHV13_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDV1241403.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTTVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            12487..12885
                     /gene="fadM"
                     /locus_tag="RHV13_001865"
     CDS             12487..12885
                     /gene="fadM"
                     /locus_tag="RHV13_001865"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HDV1241404.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFHWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(12937..13632)
                     /gene="queC"
                     /locus_tag="RHV13_001866"
     CDS             complement(12937..13632)
                     /gene="queC"
                     /locus_tag="RHV13_001866"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HDV1241405.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLK"
     gene            complement(13697..15397)
                     /locus_tag="RHV13_001867"
     CDS             complement(13697..15397)
                     /locus_tag="RHV13_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HDV1241406.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSADGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPQLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            15497..16315
                     /gene="cof"
                     /locus_tag="RHV13_001868"
     CDS             15497..16315
                     /gene="cof"
                     /locus_tag="RHV13_001868"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HDV1241407.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            16468..16926
                     /gene="decR"
                     /locus_tag="RHV13_001869"
     CDS             16468..16926
                     /gene="decR"
                     /locus_tag="RHV13_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HDV1241408.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            16956..18728
                     /locus_tag="RHV13_001870"
     CDS             16956..18728
                     /locus_tag="RHV13_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HDV1241409.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGSGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            18721..20502
                     /locus_tag="RHV13_001871"
     CDS             18721..20502
                     /locus_tag="RHV13_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HDV1241410.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            20683..21021
                     /gene="glnK"
                     /locus_tag="RHV13_001872"
     CDS             20683..21021
                     /gene="glnK"
                     /locus_tag="RHV13_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HDV1241411.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            21051..22337
                     /gene="amtB"
                     /locus_tag="RHV13_001873"
     CDS             21051..22337
                     /gene="amtB"
                     /locus_tag="RHV13_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HDV1241412.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(22386..23246)
                     /gene="tesB"
                     /locus_tag="RHV13_001874"
     CDS             complement(22386..23246)
                     /gene="tesB"
                     /locus_tag="RHV13_001874"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HDV1241413.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKDTVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            23464..24036
                     /locus_tag="RHV13_001875"
     CDS             23464..24036
                     /locus_tag="RHV13_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDV1241414.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(24067..24378)
                     /locus_tag="RHV13_001876"
     CDS             complement(24067..24378)
                     /locus_tag="RHV13_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HDV1241415.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            24541..24637
                     /gene="ffs"
                     /locus_tag="RHV13_001877"
     ncRNA           24541..24637
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="RHV13_001877"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            24757..25110
                     /locus_tag="RHV13_001878"
     CDS             24757..25110
                     /locus_tag="RHV13_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HDV1241416.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(25152..26702)
                     /gene="pdeB"
                     /locus_tag="RHV13_001879"
     CDS             complement(25152..26702)
                     /gene="pdeB"
                     /locus_tag="RHV13_001879"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HDV1241417.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRGNKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(26866..27336)
                     /locus_tag="RHV13_001880"
     CDS             complement(26866..27336)
                     /locus_tag="RHV13_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HDV1241418.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(27452..28003)
                     /gene="maa"
                     /locus_tag="RHV13_001881"
     CDS             complement(27452..28003)
                     /gene="maa"
                     /locus_tag="RHV13_001881"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HDV1241419.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(28174..28392)
                     /gene="hha"
                     /locus_tag="RHV13_001882"
     CDS             complement(28174..28392)
                     /gene="hha"
                     /locus_tag="RHV13_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HDV1241420.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(28418..28792)
                     /gene="tomB"
                     /locus_tag="RHV13_001883"
     CDS             complement(28418..28792)
                     /gene="tomB"
                     /locus_tag="RHV13_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HDV1241421.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT         7308 a         6980 c         7849 g         6960 t
ORIGIN      
        1 gaaaaataga atttataaat ggcgccaata atttccaaac gctaagccgc acaaaagaac
       61 aaatattaat aagcgatgct gatgtttatt tagccgtaat aattacgccg ggtgattact
      121 acagctaaat aatattcaca gtttacgtca gatgcgtttt tcgtagcgcc aggtaatcca
      181 gtaaaccacc aagcacgaca ccgcacaggg cgactaaaac gccgacttcc agtagagcag
      241 acaggaagga gaagtgggtc aaatccaggg catccatcgt taacagcaat agccatacgg
      301 ccaaaagacc gaagcccgcc gcaagggtcc acatggcgaa ggcataacct gatggatact
      361 cggtacgtgg gataaaattg tcattgaccc gcgtgtactc cagcgtctgt ctgcagacca
      421 gcagcagaat aagtcctggt atggcggcgg caacagagaa cagatagaac gtcggccagc
      481 cgtgagcttc cacaaaccaa ccagctactg gtcctacata aacccgtcct acagcagaaa
      541 gcgccgagag tagagcaaac tgggtggcgg aaaatgactt attacacaaa gtcattaaca
      601 gcgctacaaa cgccgatgta cccatcccac cacacaggtt ttcgaaaaag acggccgcgc
      661 ccatgctgta gagatgctta tcagtcacgg aaagcagcca gtaacctgcg ttggacgcgc
      721 cttgtaaaat accgaaaatc atgagcgcac ggaacaatga caggcgctgc atcaaaattc
      781 cgccgaacaa tgcgccgacg atggtcgcca gcagaccgag cgttttgtta actacaccca
      841 cttcgccggc atcaaatccg acgccgcgaa tcaaaaaggt ggttgtcagg ctcatggcga
      901 aagcatcgcc cagcttgtac agaacgatta acagcagaat cagccacgca ttatttcggc
      961 caaagaaatc tcgcagaggt gcaacaaccg cttgttccag cgtttttggc acaggaatgg
     1021 tgtcggtcgg ttctggtgca agcaacgtcg cgataataca agggatcaac agcgccgcca
     1081 tcaaccagta catgccctgc cagcccagcc atttatctgc cagccacagg gctaagccgc
     1141 cggaaaccag catccctaaa cggtaaccca gcacgctgat tgccgcgccc gcaccacgtt
     1201 cttctgccgg gagcacatcg gttttccacg catcgaagac aatatcctgg gaggccgagc
     1261 aaaaagcgat caccaccgcc agtgccgcca tccagcggag ttgggtgcca ggttcgagaa
     1321 aacccatcgc cgcaatggcg actaataaca ggatttgcgt ggcgagcagc caaccgcgcc
     1381 gccgcccaaa aaatgggggc gtgtagcggt ccatcagcgg tgaccagagg aatttaaaaa
     1441 cgtaagcctg gcctaccaga gagaagaaac caatggtttt gagatcaata ttctcgaccg
     1501 tcatccaggc ctgtaaggtg ccggatgtca gggcgagcgg tagcccggaa gcaaaaccca
     1561 ggatcagcaa tatggctgaa cgcggctgtt gaaaaatacg taaatattga ctggacatgg
     1621 gcgtctacag gcccggcttg caccgggcca gagggcagaa attaacgcgc gttctgcttg
     1681 atgaattcgt gaatgctggt gtcctgagac atatcagcga tggtatcggt cagcacactg
     1741 ttaaccgcat cggcgatatt tttgttggag gcctggaacg caccttcaac gttgtagctg
     1801 gcacgatagt ttttggtcat tttgttgcca ttctgcgcgg tagcgatgat ggcgatatcc
     1861 gctttggtag cgatgttgta gcgcacgttg ccctgggaca cgtcagcata cagttggcta
     1921 acgatgattt gcagattaac cgggccattc ggaccaacca tgtaaccacg cgcggtcatc
     1981 tgtttttcca gcacttcttg cagcaggaaa cgcagatcgc gggaggcggt cagggtaacg
     2041 atttgattat cgcgggtgac ttttgccagc gcctgatcgg tacgctgatc ggcaccatta
     2101 atgcttacgg tgacgcccat caggcttgga tcctgctgtg gcagtgtaat cgtcggggaa
     2161 acttcaatag ttgttggcgg ttttgcgcat cctgcaagca taaacagagc aactaacggg
     2221 aagaggattt ttttgaacat gttcgggctc tcagagactc ttaagcgtgt ttggtaaaaa
     2281 ttcccgccat cataacattg ccaacggcga ggggaagtag gtaaggcatg taaattcatc
     2341 atgttgacga aataatcgcc cctggtaaaa gaaacgctga tgcgaggcct gtgtttcaat
     2401 ctttaaatca gtaaacttca tacgcttgac ggaaaaacca ggacgaaacc taaatatttg
     2461 ttgttaagct gcaatggaaa cggtaaaagc ggctagtatt taaagggatg gatgacatct
     2521 cagcgttgtc ggaggagata tttcatgatg atacgtgagc ggatagaaga aaaattaagg
     2581 gcggcgttcc aacccgtatt cctcgaagta gtggatgaaa gctatcgtca caatgtccca
     2641 gccggctctg aaagccattt taaagttgtg ctggtcagcg atcgttttac gggtgaacgt
     2701 tttctgaatc gtcatcgaat gatttacagt actttagcgg aggaactctc tactaccgtt
     2761 catgcgctgg ctctgcatac ttacactatt aaggagtggg aagggttgca ggacaccgtc
     2821 tttgcctctc ctccctgtcg tggagcagga agcatcgcgt aaaaacgcat ttgcaactgt
     2881 cggcgctttt ccagtatgtt gctaaagatt ttatgaaaaa cggcctgcgg gccgttttgt
     2941 tttgtctgga ttttgcgctt tttgcccagc attcagacga aaattgcccg ggaattgtga
     3001 aaaaatacgc gacagcgcgc aataaccgtt ctcgactcat aaaagtgatg ccgctataat
     3061 gccgcgtcct atttgaatgc tttcgggatg attctggtaa cagggaatgt gattgattat
     3121 aagaacatcc cggttccgcg aagccaacaa cctgtgcttg cggggtaaga gttgaccgag
     3181 cactgtgatt ttttgaggta acaagatgca agtttcagtt gaaaccactc aaggccttgg
     3241 ccgccgtgta acgattacta tcgctgctga cagcatcgag accgctgtta aaagcgagct
     3301 ggtcaacgtt gcgaaaaaag tacgtattga cggcttccgc aaaggcaaag tgccaatgaa
     3361 tatcgttgct cagcgttatg gcgcgtctgt acgccaggac gttctgggtg acctgatgag
     3421 ccgtaacttc attgacgcca tcattaaaga aaaaatcaat ccggctggcg caccgactta
     3481 tgttccgggc gaatacaagc tgggtgaaga cttcacctac tctgtagagt ttgaagttta
     3541 tccggaagtt gaactgcagg gtctggaagc gatcgaagtt gaaaaaccga tcgttgaagt
     3601 gaccgacgct gacgttgacg gcatgctgga tactctgcgt aaacagcagg cgacctggaa
     3661 agaaaaagac ggcgctgttg aagcagaaga ccgcgtaacc atcgacttca ccggttctgt
     3721 agacggcgaa gagttcgaag gcggtaaagc gtctgatttc gtactggcga tgggccaggg
     3781 tcgtatgatc ccgggctttg aagacggtat caaaggccac aaagctggcg aagagttcac
     3841 catcgacgtg accttcccgg aagaatacca cgcagaaaac ctgaaaggta aagcagcgaa
     3901 attcgctatc aacctgaaga aagttgaaga gcgtgaactg ccggaactga ctgcagaatt
     3961 catcaaacgt ttcggcgttg aagatggttc cgtagaaggt ctgcgcgctg aagtgcgtaa
     4021 aaacatggag cgcgagctga agagcgccat ccgtaaccgc gttaagtctc aggcgatcga
     4081 aggtctggta aaagctaacg acatcgacgt accggctgcg ctgattgaca gcgaaatcga
     4141 cgttctgcgt cgtcaggctg cacagcgttt cggtggcaac gaaaaacaag ctctggaact
     4201 gccgcgcgaa ctgttcgaag aacaggctaa acgccgcgta gttgttggcc tgctgctggg
     4261 cgaagttatc cgcaccaacc agctgaaagc tgacgaagag cgcgtgaaag gcctgatcga
     4321 agagatggct tctgcgtacg aagatccgaa agaagttatc gagttctaca gcaaaaacaa
     4381 agaactgatg gacaacatgc gcaatgttgc tctggaagaa caggctgttg aagctgtact
     4441 ggcgaaagcg aaagtgactg aaaaagaaac cactttcaac gagctgatga accagcaggc
     4501 gtaatttacg cagcataacg cgctaaattc gcacaaaggc ccgtcaccgc caggtggtgg
     4561 gctttttttt gtcatgaatt ttgcatggaa ccgtgcgaaa agcctctttc ggtgttagcg
     4621 taacaacaaa agattgttat gcttgaaata tggtgatgcc gtacccataa cacagggact
     4681 agctgataat ccgtccataa ggttacaatc ggtacagcag gttttttcaa ttttatccag
     4741 gagacggaaa tgtcatacag cggcgaacga gataactttg caccccatat ggcgctggtg
     4801 ccgatggtca ttgaacagac ctcacgaggt gagcgctctt ttgatatcta ttctcgtcta
     4861 cttaaggaac gcgtcatttt tctaactggc caggttgaag accacatggc taacctgatt
     4921 gtggcgcaga tgctgttcct ggaagcagaa aacccagaaa aagatatcta tctgtacatt
     4981 aactctccag gcggggtgat tactgccggg atgtctatct atgacaccat gcagtttatc
     5041 aagcctgatg tcagcaccat ctgtatgggc caggcggcct cgatgggcgc tttcttgctg
     5101 accgcagggg caaaaggtaa acgtttttgc ctgccgaatt cgcgcgtgat gattcaccaa
     5161 ccgttgggcg gctaccaggg ccaggcgacc gatatcgaaa ttcatgcccg tgaaattctg
     5221 aaagttaaag ggcgtatgaa tgaacttatg gcgcttcata cgggtcaatc attagaacag
     5281 attgaacgtg ataccgagcg cgatcgcttc ctttccgccc ctgaagcggt ggaatacggt
     5341 ctggtcgatt cgattctgac ccatcgtaat tgatgccaga ggcgcaactg tgccgctata
     5401 cttatccagg gcggcacaac gctgtaagcg gcttgcgcct gagaatggca tttgcgtcgt
     5461 cgtgtgcggc acaaagtaca aagaagaggt tttgacccat gacagataaa cgcaaagatg
     5521 gctcaggcaa attgctgtat tgctcttttt gcggcaaaag ccagcatgaa gtgcgcaagc
     5581 tgattgccgg tccatccgtg tatatctgcg acgaatgtgt tgatttatgt aacgacatca
     5641 ttcgcgaaga gattaaagaa gttgcaccgc atcgtgaacg cagtgcgcta ccgacgccgc
     5701 atgaaattcg taaccacctg gacgattacg ttatcggcca ggaacaggcg aaaaaagtgc
     5761 tggcggtcgc ggtatacaac cattacaaac gtctgcgcaa cggcgatacc agcaatggtg
     5821 tcgagttggg caaaagtaac attctgctga tcggtccgac tggttccggt aaaacgctgc
     5881 tggctgaaac gctggcgcgc ctgctggacg tcccgttcac catggccgac gcaaccacgc
     5941 tgaccgaagc cggttatgtg ggcgaagacg ttgaaaacat cattcagaag ctgttgcaaa
     6001 agtgcgatta cgacgtacag aaagcgcagc gcgggattgt ctacatcgat gaaatcgaca
     6061 agatttctcg taagtcagac aacccgtcta ttacccgtga cgtttccggt gaaggcgtac
     6121 agcaggcact gttgaaactg atcgaaggta cggtagctgc tgttccaccg caaggtgggc
     6181 gtaaacatcc gcagcaggaa ttcttgcagg ttgatacctc taagatcctg tttatttgtg
     6241 gcggtgcgtt tgccggtctg gataaagtga tttcccatcg tgtagaaacc ggctccggca
     6301 ttggttttgg cgcgacggta aaagcgaagt ccgacaaagc aagcgaaggc gagctgctgg
     6361 cgcaggttga accggaagat ctgatcaagt ttggtctgat ccctgagttc attggtcgtc
     6421 tgccggttgt cgcaacgttg aatgaactga gcgaagaagc tctgattcag atcctcaaag
     6481 agccgaaaaa cgccctgacc aagcagtatc aggcgctgtt taatctggaa ggcgtggatc
     6541 tggaattccg tgacgaggcg ctggatgcta tcgctaagaa agcgatggcg cgtaaaaccg
     6601 gtgcccgtgg cctgcgttcc atcgtagaag ccgcactgct cgataccatg tacgatctgc
     6661 cgtccatgga agatgtcgaa aaagtggtta tcgacgagtc ggtaattgat ggtcaaagca
     6721 aaccgttgct gatttatggc aagccggaag cgcaacaggc atctggtgaa taattaacca
     6781 ttcccataca attagttaac caaaaagggg ggattttatc tcccctttaa tttttcctct
     6841 attctcggcg ttgaatgtgg gggaaacatc cccatatact gacgtacatg ttaatagatg
     6901 gcgtgaagca cagtcgtgtc atctgattac ctggcggaaa ttaaactaag agagagctct
     6961 atgaatcctg agcgttctga acgcattgaa atccccgtat tgccgctgcg cgatgtggtg
     7021 gtttatccgc acatggtcat ccccttattt gtcgggcggg aaaaatctat ccgttgtctg
     7081 gaagcggcga tggaccatga taaaaaaatt atgctggtcg cgcagaaaga agcttcaacg
     7141 gatgagccgg gtgtaaacga tcttttcacc gtcgggaccg tggcctctat attgcagatg
     7201 ctgaagctgc ctgacggcac cgtcaaagtg ctggtcgagg ggttacagcg cgcgcgtatt
     7261 tctgcgctct ctgacaatgg cgaacacttt tctgcgaagg cggagtatct ggagtcgccg
     7321 accattgatg agcgagaaca ggaagtgctg gtgcgtactg caatcagcca gttcgaaggc
     7381 tacatcaagc tgaacaaaaa aatcccacca gaagtgctga cgtcgctgaa tagcatcgac
     7441 gatccggcgc gtctggcgga taccattgct gcacatatgc cgctgaaact ggctgacaaa
     7501 cagtccgttc tggagatgtc cgacgttaac gaacgtctgg aatatctgat ggcaatgatg
     7561 gaatcggaaa tcgatctgct gcaggttgag aaacgcattc gcaaccgcgt taaaaagcag
     7621 atggagaaat cccagcgtga gtactatctg aacgagcaaa tgaaagctat tcagaaagaa
     7681 ctcggtgaaa tggacgacgc gccggacgaa aacgaagccc tgaagcgcaa aatcgacgcg
     7741 gcgaagatgc cgaaagaggc aaaagagaaa gcggaagcag agttgcagaa gctgaaaatg
     7801 atgtctccga tgtcggcaga agcgaccgta gtgcgtggtt atatcgactg gatggtacag
     7861 gtaccgtgga atgcgcgcag caaggtcaaa aaagacctgc gtcaggcgca ggaaatcctt
     7921 gataccgacc attatggtct ggagcgcgtg aaagatcgaa tccttgagta tcttgcggtt
     7981 caaagccgtg tcaacaaaat caagggaccg atcctctgcc tggtagggcc gccgggggta
     8041 ggtaaaacct ccctgggtca gtccatcgcc aaagccactg ggcgtaaata tgtccgtatg
     8101 gcgctgggcg gcgtgcgtga tgaagcggaa atccgtggtc accgccgtac ttacatcggt
     8161 tctatgccgg gtaaactgat ccagaaaatg gcgaaagtgg gcgtgaaaaa cccgctgttc
     8221 ctgctcgatg agatcgacaa aatgtcttct gacatgcgag gcgatccggc ctctgcactg
     8281 cttgaagtgc tcgatccaga gcagaacgta gcgttcagcg accactacct ggaagtggat
     8341 tacgatctca gcgacgtgat gtttgtcgcg acgtcgaact ccatgaacat tccggcaccg
     8401 ctgctggatc gtatggaagt gattcgcctc tccggttata ccgaagatga aaaactgaac
     8461 atcgccaaac gtcacctgct gccgaagcag attgaacgta atgcactgaa aaaaggtgag
     8521 ctgaccgtcg acgatagcgc cattatcggc attattcgtt actacacccg tgaggcgggc
     8581 gtgcgtggtc tggagcgtga aatctccaaa ctgtgccgta aagcggttaa gcagttactg
     8641 ctcgataagt cattaaaaca tatcgaaatt aacggcgata acctgcatga ctatctcggt
     8701 gttcagcgtt ttgactatgg acgcgcggat aacgaaaacc gtgtcggtca ggtaaccggt
     8761 ctggcgtgga cggaagtggg cggtgacttg ctgaccattg aaaccgcgtg cgttccgggg
     8821 aaaggcaaac tgacctatac cggttcgctc ggtgaagtga tgcaggagtc cattcaggcg
     8881 gcgttaacgg tggttcgtgc gcgtgcggaa aaactgggga tcaaccctga tttctacgaa
     8941 aaacgcgaca tccacgtcca cgtaccggaa ggtgcgacgc cgaaagatgg tccgagtgct
     9001 ggtattgcta tgtgcaccgc gctggtttct tgcctgaccg gtaacccggt tcgtgccgat
     9061 gtggcaatga ccggtgagat cactctgcgt ggtcaggtac tgccgatcgg tggtttgaaa
     9121 gaaaaactac tggcagcgca tcgcggcggg attaaaacag tgttaattcc gttcgaaaat
     9181 aaacgcgatc tggaagagat tcctgacaac gtaattgccg atctggacat tcatcctgtg
     9241 aagcgcattg aggaagttct gactctggcg ctgcaaaatg aaccgtctgg catgcaggtt
     9301 gtgactgcaa aatagtgacc tcgcgcaaaa tgcactaata aaaacagggc tggcaggcta
     9361 attcgggctt gccagccttt ttttgtctcg ctaagttaga tggcggatcg ggcttgccct
     9421 tattaagggg tgttgtaagg ggatggctgg cctgatataa ctgctgcgcg ttcgtacctt
     9481 gaaggattca agtgcgatat aaattataaa gaggaagaga agagtgaata aatctcaatt
     9541 gatcgacaag attgctgcag gggctgatat ctctaaagct gcggctggcc gtgcgttaga
     9601 tgctattatt gcttccgtaa ctgaatctct gaaagaaggg gatgatgtag cactggtagg
     9661 ttttggtact tttgccgtta aagagcgtgc tgcccgtact ggccgcaacc cgcagaccgg
     9721 taaagagatc accatcgctg ccgctaaagt accgagcttc cgtgcaggta aagcactgaa
     9781 agacgcggta aactaagcgt tgtccccagt ggggatgtga cgaagttcaa gggcgcatct
     9841 actgatgtgc cttttttatt tgtattcggt gactttctgt gtcttgtggg ctgacaattg
     9901 cccccgtttc ttgtcacaat aggcctttgc gcgcatcgat acgttgcgtg aggtacacag
     9961 tcatctacag cggagtgttg ttacaccatg atggacagct tacgcacggc tgcaaacagt
    10021 ctcgtgctca agattatttt cggtatcatt atcgtgtcgt tcatattgac cggcgtgagt
    10081 ggttacctga ttggcggagg caataactac gccgcaaaag tgaatgacca ggaaatcagc
    10141 cgtgggcaat tcgagaatgc cttcaacagc gagcgtaatc gcatgcagca acagctgggc
    10201 gatcaatact ccgagctggc agcgaacgaa ggctatatga aaaccctgcg tcaacaggtg
    10261 ctgaatcgtc tgatcgacga ggcgctgctg gatcagtacg cacgtgagct gaaactgggt
    10321 atcagcgatg agcaggttaa acaggcgatt ttcgcgaccc cagccttcca ggttgacggc
    10381 aaatttgata acagccgcta taacggtatc ctcaaccaga tggggatgac cgccgatcag
    10441 tacgcccagg cgctgcgtaa ccagctcact acccaacagc tgattaacgg cgttgctggt
    10501 accgatttta tgctgaaagg tgaaaccgac gagctggcgg cactggtagc ccaacaacgc
    10561 gtggtgcgtg aagcgactat cgatgttaac gcgctggcgg cgaagcagcc tgtgaccgaa
    10621 caggaaattg ccagctacta cgaacaaaac aaaaataatt tcatgacgcc ggaacaattc
    10681 cgcgtgagtt acatcaagct ggatgccgca acgatgcagc aaccggttag cgatgcggat
    10741 atccagagct actacgatca gcatcaggat caattcaccc agccgcagcg tacccgctac
    10801 agcatcatcc agaccaaaac tgaagatgaa gcgaaagcgg tacttgatga gctgaataaa
    10861 ggcggtgatt ttgctgcatt agccaaagaa aaatctgccg atattatctc tgctcgtaac
    10921 ggcggcgata tgggttggtt agaagatgcc actatccccg acgaactgaa aaatgctggt
    10981 ctgaaagaga aaggccaact gtctggtgtc atcaaatctt cggtcggttt cctgattgta
    11041 cgtctggacg acattcagcc agcgaaagtg aagtcgttag acgaagtgcg tgacgatgtc
    11101 gcggcgaaag tgaaacacga aaaagccctc gatgcgtact acgctctgca acagaaaatt
    11161 agcgatgcgg caagcaacga caccgagtct ctggccggtg cagagcaagc agccggcgtt
    11221 aaagccactc agacgggttg gttcagcaaa gataacctgc cggaagagtt gaacttcaag
    11281 ccggttgccg acgctatctt taacggcggt ctggtaggtg aaaacggcgc gccgggcatc
    11341 aactctgaca tcatcaccgt agacggcgac cgcgcattcg tgctgcgcat cagcgagcac
    11401 aaaccggaag cggtgaaacc gttggcagat gttcaggaac aagttaaggc actggttcag
    11461 cacaacaaag ctgaacaaca ggcgaaagtg gatgctgaga aactgctggt tgatttgaaa
    11521 gccggcaaag gtgcggaagc tatgcaggct gccggtctga aatttggcga gccgaaaacc
    11581 ttaagccgtt ccggtcgtga cccgattagc caggcggcgt ttgcactgcc actgccagcg
    11641 aaagacaaac cgagctacgg tatggcgacc gatatgcaag gcaatgtggt tctgctggcg
    11701 ctggatgaag tgaaacaagg ttcaatgccg gaagatcaga aaaaagcgat ggtgcagggt
    11761 atcacccaga acaacgcaca aatcgtcttt gaagctctga tgagtaacct gcgtaaagag
    11821 gcgaaaatca aaattggcga tgcgctggaa cagcaataat cctgaagccg cctcgcaaaa
    11881 aaatgctttg cagctgtaac aagaaaaggt cgctttcgcg gccttttcca tttctgaaca
    11941 ttgccatttg tttactgttt tcactcccgt taaggtgatt ccactgttaa caaacaagga
    12001 gaaaacagta tgaaacacgg aattaaagca ctgctcatta ccctgtccct ggcctgtgcc
    12061 ggaatgtctc atagcgcgct ggcggcagct tctgtggcga aaccgacaac ggtagaaacc
    12121 aaagcggaag ctcctgcagc acaaagtaaa gcagcagtac cggcgaaagc cagtgacgaa
    12181 gaaggcaccc gggtcagcat taataatgcc agcgcggaag agttagcccg cgcgatgaat
    12241 ggcgttggcc tgaagaaagc gcaggcgatt gtcagttatc gcgaagagta cggtccgttt
    12301 aaaacggtgg aggatctaaa gcaggtgccg gggatgggca attcgctggt ggaacgtaat
    12361 ctggcggtat taaccctgta attaatttgc atagtggcaa ttttttgcca gactgaagag
    12421 gtcataccag ttatgacctc tgtacttata acagttttta taaaaacaac gtaaggttat
    12481 tgcgctatgc aaacacaaat caaagttcgt ggatatcatc tcgacgttta ccagcacgta
    12541 aacaacgccc gctaccttga atttctcgaa gaagcccgct gggatgggtt ggaaaatagc
    12601 gacagttttc actggatgac ggcccataac atcgctttcg tggtggtgaa tatcaatatt
    12661 aattatcgtc gtccggcggt attaagcgac ttgttaacca ttacgagcca gttacagcaa
    12721 ttaaacggta aaagcggcat cttaagccag gtcattacac tggagccgga agggcaggta
    12781 gtagcggatg cgcttattac ctttgtttgt attgatctta aaacgcagaa agcattagct
    12841 ctggaagggg aattgcgcga aaagctggag cagatggtta agtaaacgtt ttgtggtgcc
    12901 ggatgctcaa gccgcatccg gcgacacccg gaataattac ttcaacccgg ttttctgctt
    12961 catcgctgcc atcaccgtcg gtttatcggc cagataatga ttcaaaccgt tggcgcgtaa
    13021 attacatgcc gcacaatgac cgcaaccgtc gcctttaatg ccgttatagc aggtcaacgt
    13081 ttcgttacgg actaaatcca gtttgccgta ataatctgcc agcgcccagg tttccgcttt
    13141 atcaatccac atcagcggcg tttcaaaacg aatatctttc gccatgccca aactgacggc
    13201 atggtttagt gctttcacaa actcatcgcg gcaatccggg tagccggaga aatccgtttc
    13261 gcagacgccg gtaattacgg cttctgcttt tacctgatac gcatatattg ccgccagcgt
    13321 caggaacaaa atattacgcc ctgggacaaa cgtattcggg atgccatcgg cttcaggttc
    13381 ataatcaggc accggaatgc tgtcacgcgt caggctgctg accgccagct cgttgagcag
    13441 cgttacatca agaaccttat gcgcgcgtgc ccccagtttc agcgccagtt cgcgtgccac
    13501 gtcgatttct gcgcgatggc gctgaccgta atcgaacgtc acgcaatgga cttcatcata
    13561 ttgttgtaat gcctgcacca gacaggtggt ggaatcctgg cctccactga acacaacgac
    13621 agcacgtttc atagataatc ctgcttgaac aataaaagcg ttatggtaac gcctgcgatt
    13681 aacccggacc agctattcat tgcgacggcg cgggaagcca ggcttcggta aattcaaacc
    13741 agccgcgcgg tgtcagtcgc acaccgttca cgccgggagg ggcactaatg cgatagtgat
    13801 agttgaatag cggcgtcagc gtcgcgtctg ccattaactg gctaaaaacc gctttcaggg
    13861 cattaaatcg gttttcctca tcaggcatta cttgcaccgc gtccagtgtc gattgcaaat
    13921 gggcatatgc tggagcgtcg aaaacatgtg gccacagtgg atcgcaacgt agccattgct
    13981 ccagagtata ttccggtgct tcgccaatta atctgtcgcc catcatgagg tctgcgtgtg
    14041 ccagtagggt cgtgtcgtcc cagttttttg cgttatgaaa aataattgtg agttcacagc
    14101 cttccgctgc cagtgtcgcc tgtaggcgtt ctgccatggt atgaagttct atcggtaggt
    14161 gataaaccag cgtcaaggtt ttcggtagtt tgacttcatc cggtacttgc caatgcggaa
    14221 tagtccagcc tggcagtaat gcatgactgg cggtgatcag gttttctccg acttctaacg
    14281 tttgtaataa accggattga tgaataatgg agattacttt tcgcgcctgc cagagggaga
    14341 gttggggact tttgcgcaac gttaaatagc aaaaacctaa actgatgcca ctactgacct
    14401 ggctgacccg ttgcagctcc tccggtttgc cgatggtgat ttgcacggga tgccgacaac
    14461 tggttcccaa atctttttcg aaaagcggcg gagttatcca gtactcaacc gctttaagca
    14521 gcggatgacg taaatggtaa taatcatggc tttccaggcg caccagctcc gctgtgaatt
    14581 gtgttaagcg aaaaggaccc gtgccgatca gtgggaattg cggatgcgcc agatggctgc
    14641 aatagctcgc cagccggtgc gcaagccagt aatcagggcg atgtaaaaag aaggtcagac
    14701 actgcggatg ggtgacctca atacgcttca cgctaataaa taattgatcc agtgctggca
    14761 gttgtaacag catcaataat cgctggtgta agtgtgaggc ttttactgca tcgccgttat
    14821 gccagtgtag ggttgaacga agataaaagt cccagcgtaa cccgtcagca gaggtttccc
    14881 agtgatgcgc taaatcgcca atcgggcgct gggtattatt atcgaagcgg gtcaggccgg
    14941 aaaatatctg tccggcgaga tgctgctcgg cacggccggg caaaaagccc ggttgtagcg
    15001 gttcgagcgg gcgatagtag ggaatacgca acgtgggcgt atcgttttgc cattgtccgc
    15061 ccataaacgg ctgtaacaga gtgcgcagct cacctggggc cagttgcgcc agctccagca
    15121 catcttgctg ctttccggtt tccagtgcct gttccatcat cgcattgcgt agcgattctg
    15181 gcgtgaccag aaagcgtagt tgtccgcgct ttccgcgtcc tgactgcgcc tgccactcca
    15241 gccatcccgc ctcctgtgcc tgacgcaaca gcgtacgaac atggcgttcg ctgcaaaaac
    15301 agcgttcggc cagttcgctg acggtgacgg tttgcggctt tccggctgaa ggttgccaca
    15361 gacgttgata ctggttaaga cggttgagca gtcgcatata aacccggaac aatattattt
    15421 aactattcac tattacttcc gtatatatca ggtgatactc aatcaccatt aaccgtgtca
    15481 cagagtggag aaagaaatgg ctcgtctggc agcatttgat atggatggaa ctttattgat
    15541 gcctgaccat catttaggtg agaaaaccct ctctactttg gcgcgactgc gtgaacgcga
    15601 cattaccctc acttttgcca cggggcgtca tgcgctggag atgcagcata ttctcggggc
    15661 gctatcgctg gatgcatatt tgattaccgg caacggaacg cgcgtgcatt ctctggaagg
    15721 tgaactttta catcgtgatg atttacctgc ggatgtcgcg gagctggtgc tgtatcagca
    15781 atgggatacc cgagccagca tgcatatctt caatgacgac ggttggttta ccgggaaaga
    15841 gatccctgcg ttgttgcagg catttgtcta tagcggtttt cgttatcaaa taatcgatgt
    15901 caaaaaaatg ccactcggca gcgtcaccaa gatctgcttc tgtggcgatc acgacgatct
    15961 tacacgcttg cagatccagc tatacgaagc attaggcgag cgtgcacatt tgtgtttttc
    16021 cgccacggat tgcctcgaag tgctgccggt gggctgcaat aaaggcgctg cattgacggt
    16081 gctgacccaa catttaggtt tatcgttgcg cgattgcatg gcctttggtg atgcgatgaa
    16141 cgatcgcgaa atgttaggca gcgtcggtag cggatttatt atgggcaatg cgatgccgca
    16201 actacgcgcg gagctcccgc atttaccggt gattggacat tgtcgaaatc aggctgtctc
    16261 tcactatttg acgcactggc tggactatcc acatctacct tattcccccg aataacgaga
    16321 tcccttccag caccgggcaa ttgcccggtt ttttttgcgt tgaatttgtc attttgtgcc
    16381 gtggtgttta aaccgcacag aataaattgt cgcgatttca cctttaaaat agaattaaaa
    16441 gagaaaaaaa ttctctgtgg aagggctatg ttagataaaa ttgaccgtaa gctgctggcc
    16501 ttactgcagc aggattgcac cctctctttg caggcactgg ctgaagccgt taatctgaca
    16561 accacccctt gctggaagcg cctgaaacgg ctggaggacg acggtatcct tatcggcaaa
    16621 gtcgccctgc tggatccgga aaaaataggc ctcggcctga ccgcctttgt gctgataaaa
    16681 acgcaacatc acagcagcga atggtattgc cgctttgtca cggtggttac cgaaatgcca
    16741 gaagtgctgg ggttctggcg catggccggt gaatacgatt atctgatgcg cgtccaggtt
    16801 gccgacatga aacgctacga cgagttttat aagcgtctgg taaacagcgt gccggggctg
    16861 tcggacgtca cttccagctt cgcgatggaa cagattaaat acaccacttc tttacccatc
    16921 gaataaatat ccagaatcag gtcaggacac aacgcgtgcg attatttgct caattaagct
    16981 ggtatttccg tcgggaatgg cgtcgctatc tcggggctgt cgccttgctt gtcattatcg
    17041 cgatgctgca actggttcca ccaaaagtgg ttggtattgt tgtcgatggc gtgacagaac
    17101 aacactttac taccgggcag atcctgatgt ggatcgccac catggtgctg attgccgttg
    17161 tggtttatct cctgcgttac gtctggcggg tattgctgtt tggtgcgtct tatcaactgg
    17221 ctgttgaact gcgtgaagat tattaccgtc agctaagccg gcagcatcct gagttttacc
    17281 tgcgtcatcg cacgggtgat ctcatggctc gtgcgaccaa tgacgtcgat cgcgtcgtgt
    17341 ttgccgccgg agaaggggtg ctgacgctgg tggattcact ggtgatgggc tgcgctgtgt
    17401 tgattatgat gtctacgcaa attagctggc agttgacctt atttgccctg ttgccgatgc
    17461 cagtgatggc gatcatgatc aagcgcaacg gcgatgcctt gcatgaacgc tttaagctgg
    17521 cacaggcggc gttttccagt cttaatgacc gcacccagga aagcctcacc agtatccgca
    17581 tgatcaaagc ctttggtctg gaagatcgcc agtcggcgtt atttgccgcg gatgccgaag
    17641 ataccggcaa aaaaaacatg cgggtggcgc gtattgatgc tcgtttcgac ccgacaatct
    17701 atatcgcgat tggtatggcg aacttgctgg cgattggcag cggtagttgg atggtggtgc
    17761 agggcagttt aacgctgggc cagctcacca gttttatgat gtatttaggt ctgatgattt
    17821 ggccaatgct ggcgctggca tggatgttta acattgtgga acgtggtagt gctgcgtaca
    17881 gccgtattcg tgcgatgctg gcggaagcgc cggtggtgaa cgatggtagc gaacccgtgc
    17941 cggaagggcg tggcgaactt gatgtaaata ttcaccagtt cacgtatccg cagactgacc
    18001 atcctgcgct ggaaaacgtc aatttcgccc tgaaacccgg tcagatgctg ggtatctgcg
    18061 ggccgactgg ttccggcaaa agtaccctgt tgtcgctcat tcagcgtcat ttcgacgtca
    18121 gcgaggggga tattcgcttt catgatattc ctctgacgaa gttacaactc gatagctggc
    18181 gtagccgcct ggcggtagtt agccagacac cgttcctttt ttccgacacc gtagcgaaca
    18241 acatcgcgtt gggttgcccg aatgccaccc agcaagagat tgagcatgtc gcgcggttag
    18301 ccagcgtaca tgacgatatt ttgcgtttac cgcaaggtta cgatacagag gtgggcgagc
    18361 gcggcgtgat gctttccggc gggcaaaaac agcgtatctc tattgctcgt gcgttattag
    18421 tcaacgcgga aatcctcatc cttgatgatg cgctttcggc ggtggacgga cgcacagagc
    18481 accagatcct gcataacctg cgtcagtggg gacagggaag aacggtaatc atcagtgccc
    18541 atcgcctttc ggcactgacg gaagccagtg aaattattgt gatgcagcac ggacatatcg
    18601 cccagcgtgg caatcatgat gagctggcgc aacaaagcgg ctggtatcgc gatatgtatc
    18661 gctatcaaca actggaggcg gcgctcgacg acgctccgga aattcgcgag gaggccgtcg
    18721 atgcgtagtt ttagccaact gtggccgact ctcaagcgtc tgttagcgta cggttcgccg
    18781 tggcgtaaac cgctggggat tgcggtcctg atgatgtggg ttgcggcggc ggcagaagtc
    18841 agtgggccgc tgcttatcag ctattttatc gacaatatgg tagcgaaaaa taacctgccg
    18901 ttgaaagtgg ttgcagggct ggctgcggca tatgttgggc tacaactgtt tgccgctggg
    18961 ctacattacg cgcagtcgct gctgtttaat cgggcggcag taggcgtagt gcaacagttg
    19021 cgtaccgacg tgatggatgc tgcgttacgc cagccattaa gcgagtttga tacccaaccc
    19081 gtcgggcagg tgatttcccg cgtcactaat gacactgaag tgatccgcga tctctacgtt
    19141 accgtagtgg caactgtcct gcgtagcgcc gcactggtgg gcgcgatgct ggtggcgatg
    19201 ttcagcctcg actggcgaat ggcgctggtg gcgataatga ttttcccggt ggtgctggtg
    19261 gtaatggtga tataccagcg ttacagcacg ccgattgtcc gtcgtgtgcg cgcctatctg
    19321 gcggatatca acgacggctt taacgaaatc atcaatggca tgagcgttat ccagcagttt
    19381 cgtcagcagg cgcgatttgg cgaacgtatg ggggaggcca gtcgttcaca ctatatggcg
    19441 aggatgcaaa ccctgcgcct cgacggtttt ctgctgcgtc cgctgctgag tctgttttca
    19501 tcgctcattc tttgtggctt gttgatgctg tttggcttct ccgccagcgg caccattgaa
    19561 gtgggtgtgt tgtatgcgtt tatcagctat cttgggcgac ttaacgaacc cttaatcgaa
    19621 ctgaccacgc agcaggcgat gctgcaacag gctgttgttg ctggtgagcg cgtgtttgag
    19681 ctgatggacg gaccgcgcca gcaatatggc aatgatgatc gcccgttaca gagtggcacc
    19741 atcgaagtcg ataacgtgtc atttgcttat cgcgatgaca atctggtgct aaagaacatt
    19801 aatctctctg tgccttcgcg caattttgtg gcgctggtcg ggcataccgg cagtggcaaa
    19861 agcaccctcg ccagtttatt gatgggctat tacccgctaa cggaaggtga gattcgcctt
    19921 gatggtcgtc cattaagttc gctaagtcac agcgcgctgc gccagggcgt ggcaatggtg
    19981 cagcaagatc cggtggtgct ggcggatacc ttcctcgcca acgtgacgct ggggcgtgat
    20041 atctccgaag aacgcgtctg gcaggcgctg gaaaccgtgc aactggcgga gctggcgcgc
    20101 agcatgagtg atggtattta tacgccgctg ggcgagcagg ggaataatct ctccgtcggg
    20161 caaaagcaac tgctggcact ggcacgcgtg ttggtggaga cgccgcaaat cctgatcctt
    20221 gatgaggcaa ccgccagcat tgactccggt actgaacagg cgattcaaca tgctctggcg
    20281 gctgtgcgtg aacataccac gctggtggtg attgctcacc gcttatcgac cattgttgat
    20341 gccgacacca ttctggtgct tcatcgtggg caagccgtgg agcagggcac tcaccagcaa
    20401 ctgctggcgg cccagggacg ctactggcag atgtatcaac tgcaacttgc gggcgaagag
    20461 ctggcagcca gcgtgcgtga agaagaatca ttgagcgcct gaatagcgca atatttcatc
    20521 gttggtgcaa aaatgtaacg cactgtgcac tgtcatagtg cgttttcatt ttcaatcttc
    20581 ttaacttcct gctctctttc tcgtttttca tttctggcac accgcttgca ataccttctt
    20641 cgtgtagcag aaccattacc gaattctgac cggaggggat ctatgaagct ggtgaccgtg
    20701 ataatcaaac cattcaagct ggaagacgtt cgtgaagcgt tatcttccat tggtattcag
    20761 ggcctgaccg tcaccgaagt gaaaggtttc gggcgtcaga aagggcatgc cgagctgtac
    20821 cggggggcgg aatacagcgt caacttcctg ccaaaagtaa aaattgatgt ggcgattgct
    20881 gatgaccaac tcgatgaagt gatcgatatc gtcagtaagg cggcttacac cggaaaaatt
    20941 ggcgacggca aaatcttcgt cgctgaattg caacgcgtca ttcgtattcg taccggcgaa
    21001 gccgacgaag cggcgctgta atctctggca cacagcaaca ggaacgaaaa atgaagatag
    21061 cgacgataaa aactgggctt gcttcactgg cgatgctgcc gggactggta atggctgcac
    21121 ctgcggtggc cgataaagcc gacaatgcgt ttatgatgat ttgtactgcg ctggtgctgt
    21181 ttatgactat tccggggatt gccctgtttt acggtgggtt gattcgcggc aaaaacgtgc
    21241 tgtcgatgct gacgcaggtg acggtgacgt ttgcactggt ctgtattctc tgggtggttt
    21301 acggttactc gctggcgttt ggtgagggca acaacttctt cggcaacatt aactggttga
    21361 tgctaaaaaa catcgaactg acggcggtga tgggcagcat ttatcagtat atccacgtgg
    21421 cgtttcaggg ctcgtttgcc tgcattaccg tcggcttgat cgttggggcg ctggcggaac
    21481 gaatccgctt ctcagctgtg ttgattttcg tggtggtatg gctgacgctc tcttacattc
    21541 cgattgcgca tatggtgtgg ggcggtggtt tgctggcttc tcacggtgcg ctggatttcg
    21601 cgggtggcac ggtggtgcat attaacgccg caatcgccgg tctggtgggc gcgtatctga
    21661 taggcaaacg cgtgggcttc ggcaaagagg cgtttaaacc gcacaacctg ccaatggtct
    21721 tcaccggtac tgccattctc tatattggtt ggtttggctt taacgccggg tcagcgggca
    21781 cggcgaatga aatcgcggca ctggcatttg tgaatactgt ggtcgcaacg gcggcggcaa
    21841 ttcttggctg gatcttcggt gaatgggcgc tgcgtggtaa gccttctctg cttggagcgt
    21901 gttctggcgc gattgccggt ctggtcggcg tgacgccagc gtgtggctac attggggttg
    21961 gcggcgcgtt gattatcggc gtggtagctg gtctggcggg cttgtggggc gttactatgc
    22021 tcaaacgctt gctgcgggtg gatgatccct gcgatgtctt cggtgtgcac ggcgtttgtg
    22081 gcattgtcgg ctgtatcatg actgggattt ttgccgccag ttcgctgggt ggcgtgggct
    22141 tcgctgaagg tgtgacgatg ggccatcagt tgctggtaca gctggaaagc atcgccatta
    22201 cgatcgtctg gtccggtgtg gtggcattta ttggctacaa actggcggat ctgacggttg
    22261 gtctgcgtgt accggaagag caggagcgag aagggctgga tgtcaacagt cacggcgaga
    22321 atgcctataa cgcgtaacaa gcactgcaaa aaacagccgg acggttttca cctccggcta
    22381 tttttttaat tgtgattacg catcacccct tcctgaacgg tcgaggcaac cagtacgccg
    22441 tcctgggtat aaaactcacc gcgcacaaag ccacgtgcgc tggacgccga ggtgctctcc
    22501 acgctataca gcagccattc attcaaatta aacgggcgat ggaaccacat ggaatggtca
    22561 atggtggcaa tctgaatccc cggttcgaga aaaccgatgc cgtgcggctg tagagctacc
    22621 ggcaggaagt taagatcaga agcgtaaccg agcagatact gatgaacgcg caggtcatcc
    22681 ggcacgctac catttgcgcg gatccacacc tgacgatgtg gttctgcgac gtgacctttc
    22741 agtgggttat gaaactccac cggacggact tccagcggac gatcgcagat gaatttatct
    22801 ttaagcactg gcggcagcag gtgcgccagc gattgggcga tttgcgtttc cgaaggcagg
    22861 ccatcaggcg ctggcgcgga cggcattgtt ttttgatgtt cgaaacccgc ttctggtgcc
    22921 tggaaagagg ccgtcatata aaaaatcggt ttgccgtttt gaatagcagc aacccggcgg
    22981 gcgctgaagc tgttaccgtc acgcagcgtt tcgacatcat aaataatcgg cttcttacta
    23041 tcgccagggc gaagaaagta gctgtgaaac gaatgtacca gccgctcttc agggacggtg
    23101 tcttttgcag catacaaggc ctgacccacg acctggccgc caaacacctg gcgtaaacct
    23161 aaatcttcac tctggccgcg aaagagtcct tcctcaattt tttccagatt taacaatgtc
    23221 agtaaatttt ttagcgcctg actcatataa ctctccagta acaaagctgc cgcagcaagc
    23281 caaagtgagt tgagtataac gcaaatttgc tactggtccg atgggtgcaa tggtctgaat
    23341 tacgggctta ttacaggcag aaatgcgtga tgtgtgccac acttgttgac gttactattt
    23401 tgttaaccac tcttccggcg aggaaagtta gcccgctggt gcattgataa taaggagaaa
    23461 tgaatgaaac tcgtgcacat ggccagtggt ttagcggttg cgattgcgtt ggcggcttgc
    23521 gcagataaaa gcgcggatat tcagacgcca gccccggctg caaatacgtc tatttcagca
    23581 acacaacaac ctgctatcca gcaaccgaat gtctccggta ccgtctggat ccggcagaaa
    23641 gtcgcactgc cgcctgatgc tgtgctgacc gtgacacttt ctgacgcgtc gttagccgat
    23701 gcaccgtcaa aagtgctggc gcagaaagcg gtgcgtaccg aaggtaaaca gtcaccattc
    23761 agctttgttc tgccatttaa tccggcagat gttcagccga acgcgcgtat tctgttgagt
    23821 gcggcgatta ccgtgaatga caaactggta tttattaccg ataccgttca gccggtgatc
    23881 aacaagggcg gaactaaagc cgacctgaca ttggtgccgg tgcagcaaac cgccgtgccg
    23941 gttcaggcca gcggtggtgc aacgactacc gtaccttcga cttcaccaac tcaggtgaat
    24001 ccgtcttcgg cagttccagc tcctacgcaa tattaagcga cgttaaccct ctccgcccgg
    24061 agagggttag tagttccagc gataacgctg caagtcgatt tgcccgcttc ccgataccat
    24121 cacaccttct gccagtaatg cctgtcgctg acgctgtaaa tccggtccgg ttagcgaaat
    24181 tgtgccgtgg cgattaacca cccggtgcca gggtaaggtg ctgccttcag ggagacgctt
    24241 taacacaccg cccacctggc gcgcggcgcg gggcgatccc gccagtttcg ccacatcacc
    24301 gtaagtggtg acatagcctt cgggaatagc ggcgacgatt tgccagacgc gttggggaaa
    24361 tgaatcttct ttttccatct tttcttcctg aggtaatttt tcagcataat ctggaaaaac
    24421 gcccgagtga agtcgcattg cgcaagaaac cagcatctgg cacgcgatgg gttgcaatta
    24481 gccggggcag cagtgataat gcgcctgcgc gttggttctc aacgctctca atgggggctc
    24541 tgttggttct cccgcaacgc tactctgttt accaggtcag gtccggaagg aagcagccaa
    24601 ggcagatgac gcgtgtgccg ggatgtagct ggcagggccc ccacccattt ctgcctccca
    24661 ccgtttcgtc aaaaaacccc aacatggcta aactttaacc acaactgacg tcgcaagaat
    24721 tgtctggctg cgcagtacgc ttcggaggta tgtctgatga agtatgttga tggttttgtg
    24781 gttgccgttc ctgccgataa aaaggatgcc tatcgggaaa tggccgctaa ggctgcgcca
    24841 ttgtttaaag agtttggcgc gcttcgtatt gtcgaatgct gggccagcga tgtaccggat
    24901 ggcaaagtga ccgattttcg tatggcggtg aaagcggaag agaatgaaga ggttgtcttt
    24961 agctggattg aatacccttc aaaagaggtc cgcgacgctg ctaatcaaaa gatgatgtcg
    25021 gacccacgga tgaaagagtt cggcgagtcc atgccgtttg acggcaagcg aatgatctat
    25081 ggcggattcg agtcaatcat cgacgaatag gtagcgtgac gggctgtgcg gcgtgccgtc
    25141 agcccaaaaa ttcacaaatg ttgctcggcc cagcgtaaga aatcttcttt cggtaatgcc
    25201 ttgctgtaga gccagccctg accgtagtgc acgccatgct ggcgtagcca ctcttcttgt
    25261 ttactggttt cgattccctc cgctaccatt ttcagtttca gtgtttttgc catttcgatg
    25321 atatgcggcg tgacattttt atattccagc gcatcaacga aagatttatc gatcttcaga
    25381 atgtcgacat ccagatcctg taaataactt aaacttgaat atcccgtacc aaaatcatca
    25441 agataaattt catggcccgc ctcccggtag cgagaaatta tcggggcgct ggttttcgga
    25501 tcggcaaact cgcgttcagt gagttcaagc gcgatctgtc tgggattaac ctgatagtga
    25561 ttgatcattt cacgcagcaa ttgcgggatt ttttccgagg tgagcacggt ggattcaaga
    25621 ttgatcgaaa tatgctgctg tggatgctga cgcagccagt cgcccatatc ttcaaagacg
    25681 cttcttataa tcagtagcgt caatggctca gaaaggcccg tttgctgtgc cagcggaata
    25741 aaactatctg gtgacaacca actaccgtct gtctgcggcc agcgcgccag tgcctcagca
    25801 ccgacaattt tgccattggc taaggagaca atcggctgat agtgcacgca aatatcacga
    25861 ttttcgatag catcctgcag ccgatgatgc ggtgactgga tacggcgcaa aatacgcagc
    25921 acaaacatcg ctgccagcag gccaatcacc aacccgagcg gtaaccaaat aaagacttgc
    25981 cgatgccagc ctttctgcaa cattttcgtt gaagcccagg tgatgatcga aatattcatc
    26041 tccgggaagg gcaggatatc gtaaatgatt ccattatttt cgatatgctc accgggtgtt
    26101 ttttgtagcc tggtaataat tccctgagca atttcatcgc tgctggttat gacaacgtta
    26161 tgggcattgc caataatggc ggcatcaatt tgccatgagc tatagggaat gacatcaata
    26221 aaggaagcgg ggtcgatcat gacgacataa tgtgccgttc ccatggcgac catgtaacgg
    26281 ataatgccta aatcgttatg cgatgttaac cagacacgat aaccatcttt cgaaatttta
    26341 cctggctcgg ggaaggtatc gggcggactt tcatgctcca gagacgaaca ctggggaacg
    26401 ttgttatcga tataaaccac ttcctgaata tagcgataac tgtaagatac ccgacgcatt
    26461 tccatgagat gggcttcgct acaggcagcg ccttgccatc tttccagctc ttgcagcgca
    26521 tctttccctt gtgtcgccac cttattgccc cgaatagcga cgcgggagga ataggtatcc
    26581 agctcttcaa taaacgatgt ttctacctgc tgatgggcca gccagatgct taagccgaca
    26641 ggcagcaata ctgaaagaat cagtactccc gaaataaggc cgaccagatg tcgtgttctc
    26701 accagcatat ccttattaac acagtgtagg gaaagtatat ctgattgtgg aaaaaggctg
    26761 gcaggagata aggaggtaga gctggtattt catgaagtga tgggtggaaa cgatcattgt
    26821 catttgacca ggcgcagagc tttacgcctg gtatgtaacc ctaacttaag atgttgattc
    26881 ggcgcgagcg agggtaaaaa tatcgtaaaa agacagttca cctttacgga cgatcatttt
    26941 ttgcagttgt tccttatgaa catccgcgac acgcggagag ttcaagatgg cttcaaccag
    27001 cgcggcaggt acaaagcgcg tgttgtacca ttcgccgtta tagcaaacgc ggaaatccag
    27061 cacgtcgata tcttcatagc ggtagcgtgg gtagacatcg aaaaagaaga aaccattaag
    27121 caggataaat aatacaacca gtagccagac agagcctgac agcgtttcgg actgcaacat
    27181 caccgccagg gtggcaaaaa aagcaacgta cataccgata aacagccccg gatgtttacg
    27241 gataaaactg atactaaagc ggggtttgtt gtcgcgtttt tcgcgggtat tcagagactc
    27301 aatcgtttcg gtcagcaggc gttgtatttc ggtcatcgtt tgccttcgtg gtatctgcaa
    27361 ctttccaggg aacaccctat tttaacgtgg gaatagtaga ggggaagtaa cagatttacc
    27421 acaattttgc ataacagttg cgaaaaaccg attacaattt tttaattatt ctggctggat
    27481 taccgcccac gacaacgttg tccgggacat cttttgtgac aactgcacct gaggctacca
    27541 cgacgttatc accaatggtc acaccagggt taatgaccgc gcgtccgcca atccagacgt
    27601 tattaccgat ggtgacgggt ttccccagtt cagcaccgct attacgtgct acagggtcga
    27661 tgggatgtgt tgccgtgtag atatgaacgc ctggtgccaa catacagtta tcaccgatgc
    27721 gaatagggca gacatcaagc atcacgcaat cgaagttggc gaaaaaatta ttaccgagaa
    27781 aaatgttata gccatagtca cagcgaaacg ttggctcaat ataagcctct gtcacctgac
    27841 cgaatagatc agcgagaatt tgctggcgta atgtgtgctc ttccgccagg gaatgattgt
    27901 atcggtgaat aagctgacga gcgcgcaggc gatcgcgaga taacgtctca tctgccgagc
    27961 gatacaactc accagcaatc atcttttctt tttctgtgct cattactgac ctccggttgc
    28021 tgaaaatgcg gcaacacagt aagacgaaaa gtcagtgaag agaacgttcc cattacatct
    28081 gtgtgataaa gatcacatag ggtatgtctt catggtgaaa agtataaaat tcttaataaa
    28141 cagccggtta tagctccgaa agcgaattat ttattagcga ataaatttcc atactgagga
    28201 agggatcttg tcgtatagtt tattcatggt caattcggcg aggcggtgat ctgcggctga
    28261 gtaaaatacc gccagttcat tatctgataa ttcgtattta tttttctcga taacacgctc
    28321 cagcgtgtca attgtctggc aacgacgtaa acgcattaaa taatcggttt tcgttaaagg
    28381 tttttcggac atacttctac ccatggttgt aataattcta acaagataaa ctcgcaggat
    28441 tctctttcgt cgcattgaca aaacaacgga atagtcgatt acctgacttc cgccatttct
    28501 gaagatcctg catattaata ccataactgc tgaacaacat aaaggtgtca tccagatatt
    28561 cgtcgatctg ctcaatgagc ttattgtctt cattatactt aattttgtaa ttaagtgcga
    28621 aggtcgcaat atgctcaatc agttcattca actggaggtt gatcgccgag gttgggtcgt
    28681 ttacccagcc atgattgctt tcttcaaggt ttgcaaggca gtcatgatac agggtttcac
    28741 agagaaactt aagctgtgcg atatcatgtc ttttgggtga gtattcatcc ataacgcgtc
    28801 cccttcttag cggttgaact aacggacacc tttcgggatg gaaaaaactt actgacctgg
    28861 acttgccctc tcgtttgtta gcttaactat aagccactct ttgcaggttt tcatcgcatt
    28921 attacgaaaa attacaatta ggaaggaagc gggacaggac tcactttttc atactagcac
    28981 taacccgcag caggtaaaag cagtttgctc gaactattca taacatttca ggattatcgc
    29041 cagatgaaat tggtgacact aataccagga ttgctctgaa tatgacgtaa taaccga
//
LOCUS       DAPGOF010000050        28335 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.050, whole genome shotgun sequence.
ACCESSION   DAPGOF010000050 DAPGOF010000000
VERSION     DAPGOF010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.050"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(10..2994)
                     /locus_tag="RHV13_001884"
     CDS             complement(10..2994)
                     /locus_tag="RHV13_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011846847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HDV1241422.1"
                     /translation="MPRRQILSSEEQERLLVIPDDEIILTRMCFLNEPDIALINKHRR
                     PANRLGFAVLLCYLRGPGFIPDKSSAPHNGVVSRVASRLKLQPDLWPEYASREQTRWE
                     HLTELYRYLELSPFSRSMQKDCIRHLHPYAMRTDKGFMLAEEMLSWLHNNNVIFPSVE
                     VIERTLAEVVTLANRSVFSTLTAQLEKQHKSALDSLLISEGEQPSRLAWLLQPPGKIN
                     GKNVLQHIDRLNSIAALGLPDGIALSVHQNRLLKLAREGRKMSSRDLAKFTDVRRYAT
                     LVCIITEARATLTDEVIDLHERILGSLFSRAKRTQAERLQQTGKLIQSKLKQYVTVGQ
                     ALLNARESGEDPWTAIEDVLPWQEFINSVEETRFLSRKGNFDALHLITEKYSTLRKYA
                     PRMLSALQFMATPAAQALSDALDTITEMYRKQLRKVPPSAPTGFIPESWRKLVLTPSG
                     IDRKYYEFCVLNELKGALRSGDIWVKGSRRYKNFDDYLIPTAEFEKSRHNDQLQLAVQ
                     TDSQAYLQARMTLLASRLEEVNAMALAGDLPDVDISDKGVKITPLENSVPSGVSPFAD
                     LVYGMLPHPKITEILEEVDSWTGFTRHFAHLKNNNVRPKDGRLLLTTILADGINLGLT
                     KMAESCPGATRSSLEGIQAWYIRDETYSAALAELVNAQKERPLAAFWGDGTTSSSDGQ
                     NFRVGSHGRYAGQVNLKYGQEPGVQIYTHISDQYSPFYAKVISRVRDSTHVLDGLLYH
                     ESDLEITEHYTDTAGFTEHVFALMHLLGFAFAPRIRDLHDKRLFIHGKAERYPGLQSV
                     ISTTCLNIKDIESHWDEVLRLATSIKQGTVTASLMMKKLASYPKQNGLAKALREIGRI
                     ERTLFMLDWFRDPGLRRRVQAGLNKGEARNALARAVFLHRLGEIRDRGLENQSYRASG
                     LTLLTAAITLWNTVYIERAIESLKRKGIPINEQLVSHLSPLGWEHINLSGDYVWRNNL
                     KLGSGKYRSLRTVDTALYKKQS"
     gene            3154..3738
                     /locus_tag="RHV13_001885"
     CDS             3154..3738
                     /locus_tag="RHV13_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDV1241423.1"
                     /translation="MSRVFAYCRVSTLEQTTENQRREIEAAGFAVKPQRLIEEHVSGS
                     VAASERPGFARLLDRMENGDVLIVTKLDRLGRNAMDIRKTVEQLAVSDIRVHCLALGG
                     VDLTSPAGKMTMQVISAVAEFERDLLLERTHSGIARAKAAGKRFGRPSALNEEQQLTV
                     IARINAGNSISAIAREFNTTRQTILRVKAGQHSS"
     gene            4130..5608
                     /locus_tag="RHV13_001886"
     CDS             4130..5608
                     /locus_tag="RHV13_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HDV1241424.1"
                     /translation="MLLSSTRKDWLGNVRGDVLAGIVVALALIPEAIAFSIIAGVDPQ
                     VGLYSAFCIPLVMAFLGGRPAMISSATGAMALLMITLVKDHGLQYLLAASALTGVLQL
                     AAGYLKLGSLMRYVSRSVVTGFVNALAILIFMAQLPELTNVTWHVYALTAAGLGIIYL
                     FPYLNKTIPSPLICIIVLTAISMWLHLDVRTVGDMGKLPDSLPVFLIPDIPLNLDTLM
                     IILPYSAGLAVVGLLESMMTATIVDDMTDTPSDKNRECKAQGVANICTSFIGGMAGCA
                     MIGQSVINVKSGGRGRLSTLTAGVVLLLMVVFLREWVSKIPMAALVAVMIMVSIGTFS
                     WRSISNLRSHPLSTSVVMLATVVVVVATDNLAFGVLTGVLIASLNFATKVARFMAISS
                     ELEDNSRTYTVAGQVFFASSDRFMSYFDFREAVERVVINVTHAHFWDITSVSALDRVV
                     IKFRREGTEVEIKGMNDATRTIVDRFGVHDKPEEVEKLMGGH"
     gene            5627..6454
                     /locus_tag="RHV13_001887"
     CDS             5627..6454
                     /locus_tag="RHV13_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDV1241425.1"
                     /translation="MNNTVIACVDGSPSTRPVCEYAAWAAAKLGTPLALLHVLEKSET
                     PAVFDLTGAIGIDSQERLTEELVRVEGERSRLLMAQGKAVLRDCAERLKQAGQTDVQL
                     LQKHGALDDILAELGEVRLMVLGRRGTQNQVGSHLESVIRLQKKPVLIVPETFMPPAR
                     VMFAYDGSSESRKNLSRLTISPLLSGLTCYIVMVNGDTLALQDAQAVLQEAGITTEAR
                     LLEDESVTGALCRYADANDIDLMVIGAYGHSRLRRFFIGSHTTEMLSESHQPLLMLR"
     gene            complement(6525..7721)
                     /locus_tag="RHV13_001888"
                     /pseudo
     CDS             complement(6525..7721)
                     /locus_tag="RHV13_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549953.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(7933..8574)
                     /locus_tag="RHV13_001889"
     CDS             complement(7933..8574)
                     /locus_tag="RHV13_001889"
                     /inference="COORDINATES: protein motif:HMM:NF012655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDV1241426.1"
                     /translation="MARPTLHDKSETLGRAIHVFWRKGFSNTSIKDLEMALDMRPGSI
                     YASFDSKAGLFTQALSVYATHQADKTRSVIQAQQNPLDGLLTLLCCFAQEKPDGQPSY
                     ACFLVKSLLEMENDEGEMKNLIRQHLLTMESIFSDAFSDAISMGYVADPDRPEHAPSR
                     LARMLQTKIIGIRIMSLQLAQNDLLRELTDDCAQFIEELKKSPDTEILVNDGL"
     gene            8732..9439
                     /locus_tag="RHV13_001890"
     CDS             8732..9439
                     /locus_tag="RHV13_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006686258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HDV1241427.1"
                     /translation="MTDDSDTPETKGILFFDVNETLLDTASLKEYAGKRLANRQDLTD
                     VWFTSLLHYSLVETVTGSWHRFGDIAEAVLEMTAERYGISLAESDEPLSAVLASLPAH
                     PDVARGLSALSQQGFTLVALTNSSAELAESQLRSACIDGYFSKTLSVEELCKYKPDLS
                     VYNWAVHLMGRPASECMLIAAHGWDVGGAKLAGMKTAFIARKGQTLYPLAPKPDFVAR
                     DIFALSQIINSLTGENI"
     gene            9436..9987
                     /locus_tag="RHV13_001891"
     CDS             9436..9987
                     /locus_tag="RHV13_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008937815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDV1241428.1"
                     /translation="MKDFTLHTPENAPGKSKVLLENIKKRAGFVPSLYAVMAESPELV
                     EAYLKLHELFLHTSFNNDEKTVVWQAINVEHNCHYCVPAHTAIAHSMKVSDDIINALR
                     DETPLQDSRLEVLRSFTLAVIRKRGEVTENDVETFLAAGFTRKNILEVILCAAQKTIS
                     NYTNHIADTPLDAPFSKYEWKKK"
     gene            10186..10713
                     /locus_tag="RHV13_001892"
     CDS             10186..10713
                     /locus_tag="RHV13_001892"
                     /inference="COORDINATES: protein motif:HMM:NF019265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDV1241429.1"
                     /translation="MGTKATKSLIESAQRGDIKAMSALLATTQADARRYARRHCHASD
                     VDDAVQESLLIISRKVTALKVAAAFSSWLFTVIRRECQKLQRMMFRHVALDHDIAETL
                     LQERSPDSLRLDLARALRSLPAHYLDVILMRDFEELTIAEISSQLNVSVGAVKSRLHR
                     ARELIRESLISTEGY"
     gene            complement(10791..11435)
                     /locus_tag="RHV13_001893"
     CDS             complement(10791..11435)
                     /locus_tag="RHV13_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015584597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDV1241430.1"
                     /translation="MFDRYLLKKSSNIREVCAVTLQRRGVKADWISWFGFGIGMMAVP
                     LLIAHAYYWALLCIVFNRMADLLDGAVARLDGPTDRGAFLDITLDFLFYSAIPLGFAL
                     AIPANNALPAAILIYSFIGTGCSFLAFAVIAAKRGMESTAYPSKGFYYLGGLTEGSET
                     VLVLLLMCLFPGWFPLIAYGFSALCCITVVTRVVAGVSLFKNNYGKVKQTGAGI"
     gene            complement(11428..12069)
                     /locus_tag="RHV13_001894"
     CDS             complement(11428..12069)
                     /locus_tag="RHV13_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003832512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDV1241431.1"
                     /translation="MLSVRNCTIHGGDNCLLQNQCFDVAPGEILTLMGASGTGKSTLL
                     GWMIGELPPDFFGSGSLWLNGTRRDGLPTEKRRIGILFQDDLLFPHLSVGQNLALALP
                     AQLKGKRSRREYIENCLIDAELGEFYQRDPATLSGGQRARVSVLRSLIAEPEALLLDE
                     PFSRLDEHLRGRFRQFVFSHIQQRGIPALLVTHDEQDIPPDGRVKKLEERADV"
     gene            complement(12071..13686)
                     /locus_tag="RHV13_001895"
                     /pseudo
     CDS             complement(12071..13686)
                     /locus_tag="RHV13_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004860869.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(13659..14846)
                     /locus_tag="RHV13_001896"
     CDS             complement(13659..14846)
                     /locus_tag="RHV13_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDV1241432.1"
                     /translation="MLQKAGWKMWVPILALGMSLQAIADSRWNEIKQQAQGQTVYFNA
                     WGGDTAANNYLRWVATEMKQHYAITVERVPLADTADAVRRIQTEASAGRQQKGSIDLL
                     WVNGENFSTLKQAGLLYGGWAERLPNWRYVNLTLPVREDFSVPTEGAESPWGSAQLTF
                     IADRRQLSQPVSDPQALLTLAREQPGRLSYPRPPDFTGMAFLEQLLLSLSPTPQLLKQ
                     PPDVKTFALVTAPLWHYLDQLHPSLWRQGKDFPASAARMDRMLADGVLKISLTFNPAH
                     AASLIANGQLPDSAYTFGFSKGMVGNVHFVAIPVNANAKAGAQVLANFLLSPRAQIRK
                     ADPRYWGDPSVLDSDKLPPELRRTMHDISPSAGMVVPVLSEPNAGWSAALEAEWLRRY
                     GVH"
     gene            complement(14878..15948)
                     /locus_tag="RHV13_001897"
     CDS             complement(14878..15948)
                     /locus_tag="RHV13_001897"
                     /inference="COORDINATES: protein motif:HMM:NF019604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDV1241433.1"
                     /translation="MKTFGTLLIGAGHAHLEVLRHWVDVGAPFGDVALLSPSPDAWYS
                     GMLPGLLAGRYQSEQCRIPLSPLCLAAGVTLMQGKMIALDAQTGRVSLEDGRLLQGEC
                     VSLNTGAALRSPHNLSSVMDVLPVKPFADFLIRLAHWQRHPGSLAIIGGGAAGVELAL
                     AMSNQTPSLTLFCSDMLLGSHAPGVRQRAHYHLKRAGVQVQEHCPIDAIQGNGLLSGQ
                     QIVWEGNRVLLASGPAPASWWQSSGLSCDDRGFIQITTTLQSASHPHVFATGDCATLP
                     DTPKSGVYAVRQGAVLEENLRRAARQESLRPFRPQSRAVALLADGQGGAIMSWAGLSA
                     EGKWAGVWKDILDRRFIQRHSL"
     gene            complement(15945..18089)
                     /locus_tag="RHV13_001898"
     CDS             complement(15945..18089)
                     /locus_tag="RHV13_001898"
                     /inference="COORDINATES: protein
                     motif:HMM:NF014860.1,HMM:NF019604.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013097034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDV1241434.1"
                     /translation="MNLGKGLLLVAIFILAGLFLFYSSGHILSLAGIKNSQAALNHWV
                     VNRPLLSGCLFFLLYVLITALSLPGAVLMTLLAGALFGLTVGTLLASFASTLGATLAL
                     LMSRFLLRGWIQQRFARRLMAIDTNIHQGGASYLFALRLVPIFPFFLINLAMGLTRLP
                     VSTFWWVSQLGMLPGTLVYVNAGRALGQLESLTDIISPGIIGAFVLLGLLPLISRRVI
                     NHLNARKRDKPWPKPRHFDRNIIVIGAGSGGLVSAYIAAAVKAKVTLIEKGPMGGDCL
                     NTGCVPSKALIRAARLAHDLTKASALGFRHVAGEVDFSAVMERVQQVVQRIEPHDSVE
                     RYTQLGVEVISGAATVTSPWHVTVNGRQLSARSLIIATGARPVIPVIPGIEHIAPLTT
                     ENIWMLREKPQRLLILGGGPIGCELAQAFQRLGCAVTMVVRGERLLKREDRLAAEVIM
                     DSLRADGVDLRLAHQAQRFVCEGHKQWLECRNKVDGSDVVLPFDRVLLALGRRANVSG
                     FGLETLSLKIRDDGTLETDDFLATRFPHIFAVGDVTGPYQFTHAAAHQAWYAAVNALF
                     GSFKRFPVDYRIMPRTTFTDPEVAHVGLNEQEAQQAGVAYETTCFNLAELDRAITEDA
                     AHGYIQVLTAPGKDRILGATIVGEQAGELITEYVSAMKQGYGLNKILGTIHVYPTLSE
                     ANKYVAGEWKRNHAPQRVLSWLARFHQWSLGR"
     gene            complement(18381..18884)
                     /locus_tag="RHV13_001899"
     CDS             complement(18381..18884)
                     /locus_tag="RHV13_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008454705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDV1241435.1"
                     /translation="MKWAAHCRGWRTSLQQGTARYASEVVAILARLGLAATFWLSGQT
                     KVDGLHINILGGEPLQLGWPHITAGTLALFRNEYRLPVLPPEIAAVMAASAEHILSFL
                     LVIGLATRLSAAGILSMTLVIEVFVYPDAWPTHALWATSSLFLITNGSGHLSLDWLIF
                     RGINRQK"
     gene            complement(18881..19681)
                     /locus_tag="RHV13_001900"
     CDS             complement(18881..19681)
                     /locus_tag="RHV13_001900"
                     /inference="COORDINATES: protein motif:HMM:NF021364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="HDV1241436.1"
                     /translation="MQTITCDPSEPDYESAFGYPLLRPELEAPLGLRTWNGSDPAQRY
                     GIYRNNVMVSLTEALADNFPTIRTQVGEAFFTAMSACYIRQHLPDSPVLAFYGSNMPA
                     FIAAFEPLRDYPWLSDLARMDMAFIRASHAGDAAAPVYSSLPEELMMLKVVLHPSLQL
                     MCSSWAIFSLWMRDRDGDQGMETEFVDPAQPQSVMLFRPHLDVMVQPIQLASARFVDA
                     LMAGKTLMKALEDGLDCDAAFEPTLILRGLIHRGLVLNFSHDVNGETL"
     gene            complement(19653..20540)
                     /locus_tag="RHV13_001901"
     CDS             complement(19653..20540)
                     /locus_tag="RHV13_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011144487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HDV1241437.1"
                     /translation="MTAINPMPALYDGHPARAINHHLPLRAGTGLKPQHFSTIIDDQP
                     DIGFFEVHAENYLVAGGPLHHYLQRIRRHYPLSIHGVGMSIGGQAPLNRAHLEEVSRL
                     VQQYQPAAFSEHLAWSTHQTHFLNDLLPLPYTKETLLRVCSHIDQVQEKLKRPILLEN
                     PATYVEFAASTWSETAFLTEILSRTGCGLLLDINNLHISSINHHYDAVTMLQELPTEH
                     VGEIHLAGHTQSLDSMGAPLLIDSHDCQVSDPVWALYKTALRHTGPVATIIEWDSNIP
                     AFSVLMEEVKRADYHLRSL"
     gene            complement(20572..20868)
                     /locus_tag="RHV13_001902"
     CDS             complement(20572..20868)
                     /locus_tag="RHV13_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HDV1241438.1"
                     /translation="MKKHTAAVVALALGSVLASASTTAFAADKPAMEKCFGVALKGKN
                     DCKAGAGTTCAGTAKNDYQGNAFKMVPTGTCLKTDSSTSPTGKGQLQAFTEKAS"
     gene            complement(21202..22779)
                     /locus_tag="RHV13_001903"
     CDS             complement(21202..22779)
                     /locus_tag="RHV13_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HDV1241439.1"
                     /translation="MRQNLPVTGRNLELPKDANILSTTSPQSHITYVNPDFIKISGFT
                     EEELLGQPHNIVRHPDMPPAAFEHMWSTLKSGRSWMGLVKNRCKNGDHYWVSAYVTPI
                     AKNGSIVEYQSVRTKPEPEQVLAAEKLYAQLRSGKAARPKLAASFSVKILLLIWGSII
                     SSAMAAGMLTDTSISSLLLATLMSGSLSSVSVLAILSPLGRLVERARNISNNPLSQSL
                     YTGRTDEFGQIEFALRMMQAETGAIVGRIGDASNRLSEHTRGLLKDIESSNVLTVEQQ
                     AETDQIATAVNQMVASIQEVASNAQHAADAAGRADTETASGQRLVAHTSQSITALEGE
                     IRQATQVIHELEGQSNEISKVLDVIRGIAEQTNLLALNAAIEAARAGEQGRGFAVVAD
                     EVRSLAARTQQSTTDIQSMISALQERAQSAVTVMEQSSRQAHTSVAHAEEAATALDGI
                     GQRVNEITDMNAQIATAVEQQGAVSEDINRSIINIRDAADTNVQTGQNNLQSAKSVAQ
                     LTSALSELAKQFWEKRG"
     gene            23073..23138
                     /locus_tag="RHV13_001904"
                     /pseudo
     CDS             23073..23138
                     /locus_tag="RHV13_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229646.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            23213..24892
                     /locus_tag="RHV13_001905"
     CDS             23213..24892
                     /locus_tag="RHV13_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HDV1241440.1"
                     /translation="MATDTPRIPEQGVATLPDEAWERARRRAEIISPLAQSETVGHEA
                     ADMAAQALGLSRRQVYVLIRRARQGSGLVTDLVPGQSGGGKGKGRLPEPVERVIHELL
                     QKRFLTKQKRSLAAFHREVTQVCKAQKLRVPARNTVALRIASLDPRKVIRRREGQDAA
                     RDLQGVGGEPPAVTAPLEQVQIDHTVIDLIVVDDRDRQPIGRPYLTLAIDVFTRCVLG
                     MVVTLEAPSAVSVGLCLVHVACDKRPWLEGLNVEMDWQMSGKPLLLYLDNAAEFKSEA
                     LRRGCEQHGIRLDYRPLGQPHYGGIVERIIGTAMQMIHDELPGTTFSNPDQRGDYDSE
                     NKAALTLRELERWLTLAVGTYHGSVHNGLLQPPAARWAEAVARVGVPAVVTRATSFLV
                     DFLPILRRTLTRTGFVIDHIHYYADALKPWIARRERWPSFLIRRDPRDISRIWVLEPE
                     GQHYLEIPYRTLSHPAVTLWEQRQALAKLRQQGREQVDESALFRMIGQMREIVTSAQK
                     ATRKARRDADRRQHLKTSARPDKPVPPDTDIADPQADNLPPAKPFDQIEEW"
     gene            24895..25803
                     /locus_tag="RHV13_001906"
     CDS             24895..25803
                     /locus_tag="RHV13_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003155746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniB family NTP-binding protein"
                     /protein_id="HDV1241441.1"
                     /translation="MDEYPIIDLSHLLPAAQGLARLPADERIQRLRADRWIGYPRAVE
                     ALNRLEALYAWPNKQRMPNLLLVGPTNNGKSMIVEKFRRTHPASSDADQEHIPVLVVQ
                     MPSEPSVIRFYVALLAAMGAPLRPRPRLPEMEQLALALLRKVGVRMLVIDELHNVLAG
                     NSVNRREFLNLLRFLGNELRIPLVGVGTRDAYLAIRSDDQLENRFEPMMLPVWEANDD
                     CCSLLASFAASLPLRRPSPIATLDMARYLLTRSEGTIGELAHLLMAAAIVAVESGEEA
                     INHRTLSMAVYTGPSERRRQFERELM"
     gene            25800..27017
                     /locus_tag="RHV13_001907"
     CDS             25800..27017
                     /locus_tag="RHV13_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HDV1241442.1"
                     /translation="MKPAPRWPLHPAPKEGEALSSWLNRVALCYHMEEPDLLEHDLGH
                     GQVDDLDTAPPLSLLALLSQRSGIELDRLRCMSFAGWVPWLLDSLDDQIPDALETYAF
                     QLSVLLPRLRRKTRSITSWRAWLPSQPINRACPLCLSDPENQAVLLAWKLPLMLSCPL
                     HGCWLESYWGVPGRFLGWENADAEPRTASDAIAAMDQRTWQALTTGHVELPRRRIHAG
                     LWFRLLRTLLDELNTPLSACGTCAGYPRQVWEGCGHPLRAGQSLWRPYETLNPIVRLQ
                     MLEAAATAISLIEVRDISPPGEQAKLFWSEPQTGFTSGLPTKAPKPEPINHWQRAVQA
                     IDEAIIEARHNPETARSLFALASYGRRDPASLERLRATFVKEGIPPEFLSHYLPDAPF
                     ACLKQNDGLSDKF"
     gene            27079..27702
                     /locus_tag="RHV13_001908"
     CDS             27079..27702
                     /locus_tag="RHV13_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003155741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDV1241443.1"
                     /translation="MLIGYMRVSKADGSQSTNLQRDALIAAGVSLAHLYEDLASGRRD
                     DRPGLAACLKALREGDTLIVWKLDRLGRDLRHLINTVHDLTARSVGLKVLTGHGAAVD
                     TTTAAGKLVFGIFAALAEFERELISERTVAGLISARARGRKGGRPFKMTAAKLRLAMA
                     SMGQPETKVGDLCEELGITRQTLYRHVSPKGELRPDGVKLLSLGSAA"
     gene            complement(27907..28239)
                     /gene="qacG2"
                     /locus_tag="RHV13_001909"
     CDS             complement(27907..28239)
                     /gene="qacG2"
                     /locus_tag="RHV13_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacG2"
                     /protein_id="HDV1241444.1"
                     /translation="MKNWLFLATAIIFEVIATSALKSSEGFTRLVPSFIVVAGYAAAF
                     YFLSLTLKSIPVGIAYAVWSGLGIVLVTAIAWVLHGQKLDMWGFVGVGFIISGVAVLN
                     LLSKASVH"
BASE COUNT         6793 a         7760 c         7338 g         6444 t
ORIGIN      
        1 tatcccacgc taagactgtt ttttgtacaa agcggtatcg actgtacgta atgagcggta
       61 ttttccggat cccagcttaa gattattacg ccagacgtaa tctccactca gattgatatg
      121 ttcccagccc aggggagaaa gatgagagac cagttgctca tttatcggga taccctttcg
      181 ttttagtgac tcaatagctc tttctatata taccgtgttc cacaacgtga tcgctgctgt
      241 cagtaatgtc agcccgctgg cgcgataact ctgattctcc agcccacgat cccttatttc
      301 acccagacgg tgcaaaaaga ccgctcgcgc aagggcatta cgggcctcac ccttattcag
      361 ccccgcctgc acgcgtcggc gcagaccggg atcacggaac cagtccagca taaatagtgt
      421 ccgttcgatg cggccaatct ctctcagcgc tttggcaagt ccattctgtt ttgggtaact
      481 ggctaacttt ttcatcatca gtgatgcggt gactgtcccc tgcttaatcg aggttgccag
      541 gcgcaatacc tcatcccagt gcgactcaat gtctttgata ttcaggcagg ttgttgatat
      601 gacagactga agccccggat agcgctcggc ctttccatga ataaacagcc gcttgtcatg
      661 aagatcacgg atccttggcg caaaagcgaa tcccagcagg tgcatcaggg cgaaaacatg
      721 ttcagtgaag cctgcggtat cggtgtaatg ctcggtaatt tccagatcgc tttcatggta
      781 cagcaggcca tcaagcacgt gggttgagtc gcgcacccgg ctgatcactt tggcgtagaa
      841 cgggctgtat tggtctgaga tatgcgtata aatctgcacg cccggctcct gaccatattt
      901 aagattgacc tgaccggcat aacgtccgtg actgcctacc cgaaagttct gcccgtctga
      961 cgacgatgtt gtcccgtcgc cccagaatgc ggccagaggc cgctctttct gtgcgttgac
     1021 cagctcggcc agtgccgctg aataagtttc atccctgatg taccatgcct gaataccttc
     1081 gagtgacgat cttgtggccc cagggcagga ttccgccatt tttgtcagcc caaggttgat
     1141 gccgtcagcc agaatggtgg tcagcaacag tcttccgtct tttggtctga cgttattatt
     1201 tttgaggtgc gcgaagtgac gcgtaaatcc cgtccagcta tcaacttctt ccagtatctc
     1261 cgtaattttc ggatggggaa gcatgccata gaccaaatct gcaaagggcg aaacacctga
     1321 aggaacgctg ttctccagtg gagtgatttt tacgccttta tctgagatat ctacatcggg
     1381 caaatcaccg gcaagcgcca tcgcgttaac ttcttccagc cgagatgcaa gaagagtcat
     1441 acgggcctga aggtatgcct ggctatcggt ctgaacggcc aactgtaact ggtcattatg
     1501 tcgggatttc tcaaactcag cagtcgggat gagataatca tcaaaatttt tgtagcggcg
     1561 cgatcctttt acccagatat caccggaacg taatgcaccc ttgagttcat tcagtacgca
     1621 aaactcgtag tacttgcggt cgatgcctga aggcgtgagc accagttttc gccagctttc
     1681 agggataaac cctgttggcg ctgatggcgg cactttacga agttgtttac ggtacatttc
     1741 cgttatggtg tccagcgcat cgctgagcgc ctgcgccgca ggtgtcgcca tgaactgcaa
     1801 tgctgacagc atacgcgggg catatttacg caacgtactg tatttttcgg tgatcagatg
     1861 aagcgcgtcg aaattgccct tacgggaaag aaaccgcgtt tcttccacgc tgttgatgaa
     1921 ttcctgccag ggaaggacat cttctattgc agtccaggga tcttccccgg attctctggc
     1981 gttaagtaac gcctgcccga cggtaacgta ctgcttcagc ttgctctgaa taagctttcc
     2041 cgtttgctgg agccgttcgg cctgcgtgcg ttttgccctg ctgaacagac tacccaggat
     2101 acgctcgtgc agatcaatca cttcgtcagt cagggtggcc ctggcctctg ttattataca
     2161 aaccagcgta gcgtaacgtc tgacatcggt gaattttgcc aggtccctgc tgctcatttt
     2221 ccggccctca cgcgccagtt taagcaacct gttctggtga acggaaagtg caataccatc
     2281 aggcaacccc agcgcagcga tggaattaag ccggtcgata tgttgcagca catttttacc
     2341 gtttatttta cccggaggct gtagcagcca tgccagacgg gaaggttgtt caccctctga
     2401 tatgagcagg ctgtcgagtg ctgatttatg ctgcttttcc agttgcgcgg taagtgtcga
     2461 aaataccgat ctgttagcga gcgtgacgac ttcggcaagc gtccgttcga tcacttcaac
     2521 agaagggaaa ataacattat tgttatgtag ccagctgagc atttcttccg ccagcataaa
     2581 tcctttgtca gttcgcatgg cataggggtg cagatggcgg atacagtctt tttgcattga
     2641 ccggctgaac ggggatagtt ccaggtagcg ataaagttcg gtcagatgtt cccagcgggt
     2701 ttgctctctg gatgcatatt ccggccataa atcaggctga agtttcagtc gggaagcaac
     2761 cctggataca acgccattgt gaggagcact gcttttgtcc ggaataaatc caggtccacg
     2821 cagataacag agtaatacag caaagcccag gcgattcgct ggccgtcggt gcttattaat
     2881 gagcgcaata tccggttcgt tcagaaaaca cattcgggtc agaataattt catcatctgg
     2941 tataaccagt aagcgttcct gctcttcact gctcagtatc tgtcgccgtg gcataaatgc
     3001 ttccctcgcg tatagttttc atctgttatt tgttcgctgt gaccgaaacc ggtcggtttc
     3061 gtacaccagt ctgaaaatat gtccgaaagt aagcgaattt tatttcgtac aggcgctata
     3121 attcggacac cctttgagta cggaaagttt cctatgtcac gagtttttgc ttactgccgg
     3181 gtatccactc tggaacagac cacagaaaac cagcgtcgtg aaattgaggc agctggtttc
     3241 gctgttaaac ctcagcgact tattgaggaa catgtcagcg gctcagtggc tgccagtgaa
     3301 cggccaggat ttgccaggct gcttgaccgt atggaaaatg gcgatgtgct tattgtcacc
     3361 aaacttgacc gcctcggacg caacgcgatg gacatcagaa aaacggtaga gcaactggct
     3421 gtttcagata ttcgggttca ctgcctggct cttggcggtg tggatctgac cagccccgcg
     3481 gggaagatga ccatgcaggt tatttctgca gtagcggaat ttgagcggga tctgttgctc
     3541 gagcgcacgc actcaggtat tgccagggca aaagcagctg gaaagcggtt tggtcgtcct
     3601 tctgctctga atgaagagca gcaacttact gtgattgcac gtattaacgc agggaacagt
     3661 atcagcgcta ttgcccggga atttaatacc acccggcaaa ccatacttcg ggtaaaggca
     3721 ggacagcatt catcctgaat gtaaatgtag ctacgacttt acaatttgct atgtgccaga
     3781 catttcaaag aaaaatacct gaatcgaggg ttgaggagga tttatgtact gccccaaaat
     3841 ttgaccttgc ctgccattag tttatagtat tgagcggctt tggtctgatc ttggttcaga
     3901 tctgccccct ttcatttgcc tcgggcaaca gttcaggcaa taggtacact ctcgctttgg
     3961 agaatctgac aaccgttatg cgtattacgc caccctaagc ttccccttcc tccgtcgtac
     4021 tgagcgagcc tcagaacgca ctcaataaca tcccgtttgc ggatgtgttc taccgtacat
     4081 aaaaatattg ccctcaaggg cttactgtat ctaaaactga gaaattacta tgttgctgtc
     4141 ctcgacgcgt aaggactggc tgggtaacgt ccgtggtgac gtactggccg gtatagttgt
     4201 cgcgctcgcg cttattcccg aagcgatcgc cttttcaatc atcgccggag ttgacccgca
     4261 ggttgggctc tactctgcgt tctgtattcc cttggttatg gccttccttg gtggccgccc
     4321 ggcaatgata tcgtccgcta caggcgcgat ggcactgcta atgatcacac tggtaaaaga
     4381 ccacggcctg cagtatctgc tggcggcctc ggcgctgacc ggcgtgctgc aactggcggc
     4441 cggctacctg aaactgggta gcctgatgcg ctacgtctcc cgctcggtgg tcactggttt
     4501 cgtcaacgcg ctggccatcc tcatatttat ggcacagttg ccggagctca ccaacgttac
     4561 ctggcacgtc tatgcactga ccgctgcggg cctgggcatc atttatctct tcccgtatct
     4621 gaacaaaacc attccctcgc cgctgatctg catcatcgtg ctgaccgcca tttccatgtg
     4681 gcttcacctg gacgtgcgta ctgtgggtga catgggcaag ctgccggaca gcctgccggt
     4741 gtttctgatc cctgatattc cgcttaacct cgacacgctg atgattattc tgccgtactc
     4801 tgcggggctg gccgttgtgg gtcttctgga gtcgatgatg actgccacca tcgtggatga
     4861 catgaccgac acgccaagcg acaagaaccg cgaatgcaag gcacagggtg tggccaacat
     4921 ctgcacctcg tttattggtg gcatggcagg gtgcgcaatg attggccagt cagttatcaa
     4981 tgttaaatcg ggtggacgcg gacgactttc aacactgacc gccggcgtag ttctgctttt
     5041 gatggtggta ttcctgcgtg agtgggtctc caaaataccg atggcagcgc tggtcgcggt
     5101 gatgattatg gtttccatcg gtaccttctc ctggcggtct atcagcaacc tgcgcagcca
     5161 tccgctgtca accagtgtgg tgatgctggc aaccgtagtg gtagtggtgg caactgataa
     5221 cctagcgttt ggtgtactca ccggcgtgct gatagcctcg ctgaacttcg ccactaaagt
     5281 agcccgcttt atggcgatct cttctgaact ggaggacaac tcccgtacct acactgtagc
     5341 tggccaggtc ttttttgctt catccgatcg ctttatgagc tacttcgact tccgtgaagc
     5401 cgttgagcgt gtggtgatta acgtgacgca tgctcatttc tgggatatta cctccgtgag
     5461 tgcgctggac agagtagtca tcaagtttcg gcgcgaaggc acggaagtgg agattaaggg
     5521 tatgaatgac gcgacccgca ccatcgttga ccgcttcggg gttcatgaca agcctgaaga
     5581 agtggaaaaa ctgatgggcg gccattaatt tactggagaa agcatcatga ataacaccgt
     5641 tattgcctgc gtcgacggct cgccgtcaac ccgtccggtc tgtgagtatg ccgcctgggc
     5701 ggccgctaag cttggtacgc cgctggcact gctgcacgta ctggagaaga gtgagactcc
     5761 cgccgtgttt gacctgaccg gggccattgg tattgacagc caggagcgtc tgactgaaga
     5821 actggtccgc gtcgagggtg agcgcagccg gctgctgatg gcgcagggca aagccgtgct
     5881 gcgcgactgc gcggagcggc tgaagcaggc cggacaaacg gacgtacagc tgctgcaaaa
     5941 gcacggcgca ctggacgata ttctggctga gctgggggaa gtccgcctga tggtgctggg
     6001 tcgccggggt acgcaaaatc aggtggggag ccatctggag agcgtcattc gcctgcagaa
     6061 aaaaccggtg ctgattgtgc cggagacgtt catgccgccg gcacgggtga tgtttgcgta
     6121 tgacggcagc agtgagagcc gtaaaaacct gtcgcgtctg acgatcagcc cattactaag
     6181 cggccttacc tgttatattg tgatggtcaa cggtgacact ttagccctgc aggatgcgca
     6241 ggctgttctt caggaagctg gtatcacaac ggaggcccgc ctgctggaag acgagtccgt
     6301 gaccggcgcc ctgtgccgct acgcggacgc taatgatatc gacctcatgg taattggggc
     6361 ctatgggcat tcccggttac ggcggttctt tattggcagc cacaccacag aaatgctgtc
     6421 agaaagccat cagcccctgc tgatgctgcg atgaaacaga agggcctgat atcaggccct
     6481 tcttcatagg gaaagaggat gatgtgcatc acggaaaagt cgcattattg cggtgcatca
     6541 gtcctgatat cggtggccag caggctggcc ccaagaatgt agaccaggca gaacaacgcc
     6601 tgtagatgca gggtgacatg gttatctatc aggacgccca tgcttaccgg agatgccccg
     6661 cgggcaatca cggtacctgc cccaacagct gagcgcaccc tggctagctg cgccggacca
     6721 aacaactgca cccagagagc ggtggcaagc gttgaggcaa tagccgacga tacgccgatc
     6781 ataatcaggt aaaaaggcgt tacccacaga ccgttgctga gccacagcgc caacatggct
     6841 agggactgtg gcatcagtaa ccacggcaac agcctgcgtg gtgaaagacg gtcaatcagc
     6901 gggccaaact acagcaacga taccgcttgg gtaatggcgt aggcaataaa ccataccgcc
     6961 acccacgaca gcacacagcc cttctcctgc gccagcctcg cttggtggaa gaagaagccg
     7021 gttgtgataa agggggaggc cagcacgaca gacagcgcta gcagcaggtt acggtttctc
     7081 cataccgatg cctctttttc cttctcatca cccccgtttt ctgaacgtgc gcagcggctt
     7141 atctttgccg ctgggcaaaa acatcaacgc tacccccacg ccagcaacaa gcataccggc
     7201 attgatgccc caggcaaccc tccatcccgt cagcgcgctt atctgcagcg caatcacggg
     7261 aaaaacggcc tgcgccagcg agagtcccag cgcaatggcg ccgagggcct tgcctgcatc
     7321 aagggggaac tgacgcgccg ttgcggtcag tgcggttgta ccatcagtcc ctgaccgccg
     7381 aggcgcagaa agtaaaaccc gatgcagagc atccacgcag actgtgcgcc agaaatcagc
     7441 aggcaggccg tcagtaacag aagggtgact gccagcgtga agcggctggt cgtggtgtag
     7501 tcgatcatcc gtccggccca gatcagcgtg atggcgctca tcaccgttcc gatcgcgtac
     7561 aagccgccca ggctgccgtc ggtgagccca aaggcctagc gaaaatcttg cccgtaaagt
     7621 gacacaaaat aggtctgtcc aaagctggac atagctatca gcagcgcacc gaacgtaagg
     7681 tgagaagcgt tatgaagcat aaaacgaaaa agatgacgca tgagcttttt taacctgaaa
     7741 taagttgccc tggatatacc ggttttctcc atcaccattt taataggcgt ttcatgccgg
     7801 ataagctcca gtgcggcgga agatttctga tgaggtttcc gcccaaatct tacgcctgcc
     7861 gccattgcga tgactctgcc ttcgtttaat tgtgatctgc tctaaaagtc cgaattattc
     7921 aacacagcca cgctaaaggc catcatttac aagtatctca gtatccggac tctttttaag
     7981 ttcttctata aactgggcgc agtcatctgt cagttctcgt agcagatcat tttgtgcaag
     8041 ttgtagggac attattctga taccgataat tttggtttgc agcatgcgag ccaggcgcga
     8101 cggagcatgc tctggcctgt caggatccgc gacatatccc atcgaaatcg catcgctaaa
     8161 agcatcactg aaaatactct ccattgtcag caaatgctgt cttattaagt ttttcatttc
     8221 accctcgtcg ttctccattt cgagtaggct tttcaccaaa aaacaggcgt aagagggctg
     8281 gccgtctggt ttttcctgtg caaagcagca gagtagcgtc agaaggccgt ccagcgggtt
     8341 ctgctgcgcc tgaataacag acctggtttt atctgcctgg tgtgtcgcat agactgacag
     8401 agcttgcgta aacaaacctg ctttgctgtc gaaagaggca taaatacttc caggacgcat
     8461 atccagtgcc atctcaaggt cttttattga tgtgttgctg aaacctttac gccagaacac
     8521 atgaattgcc ctgcctaacg tttccgactt gtcatgtaat gttggtcttg ccatttatct
     8581 cactgaatta tataaaataa tataattatt gagtgaacgc tcaggttttt attgagtgat
     8641 cactcaaatt catctatctt aaacctactc tatttgcaga gtgcagtaaa tatccgtttt
     8701 tataaaagtc atcatccgga ccggagctca tatgacagac gatagtgaca cgccagaaac
     8761 gaaaggaatt ctcttttttg atgtgaatga aacccttctg gatactgctt cgctcaaaga
     8821 gtatgcagga aagaggcttg ccaacaggca ggaccttacc gacgtctggt tcacctcttt
     8881 gttacactat tcacttgttg aaaccgttac cggttcctgg caccgctttg gcgatattgc
     8941 agaggctgtg cttgagatga ctgccgagcg gtacggcata tctttagctg aatctgacga
     9001 gccactttcg gcagtgcttg cttcattacc tgcacatcct gatgtagcca ggggattatc
     9061 agcactgagc caacagggct ttacactggt cgccctgacc aactcatctg ctgaactcgc
     9121 tgagagccaa ctccgttcag cctgcatcga tggctatttc agtaagaccc tcagtgttga
     9181 agagttgtgt aaatacaaac ccgacctgag cgtttacaac tgggcagtcc atttgatggg
     9241 aagaccggcc tcagaatgta tgctgattgc agcacatggc tgggatgtag gaggagcaaa
     9301 acttgcgggg atgaaaaccg catttatagc cagaaaaggg caaactctct atccccttgc
     9361 acccaaacca gactttgttg ccagagatat tttcgccctt tcgcaaatca ttaattcgtt
     9421 aactggagag aatatatgaa agattttact ttacacacgc cggaaaatgc accgggaaag
     9481 agcaaggtgt tacttgagaa cataaagaaa cgtgcaggct tcgtaccctc gctctacgcg
     9541 gtaatggccg agtcgccgga gttggtggaa gcttatctta agctccatga attgtttctc
     9601 cataccagtt tcaataatga cgagaaaacc gtggtgtggc aggcaattaa tgtagagcac
     9661 aactgccatt attgtgtgcc tgctcatacg gcaattgcac attcaatgaa agtcagtgat
     9721 gacatcataa atgcattgcg ggatgaaaca ccgcttcagg atagccgcct tgaagtcctg
     9781 aggtcgttca ctcttgcggt aatacgcaag cgcggtgagg tcactgagaa tgatgtggaa
     9841 accttccttg cagcagggtt cacccgtaaa aatattcttg aggtcattct gtgcgccgct
     9901 cagaagacaa ttagcaatta cacaaaccat attgctgaca caccactaga tgcacctttc
     9961 agtaagtatg aatggaagaa gaaataattt ttcattttac ggcctgcagt atcaatgcag
    10021 gctctttact ttttaaatgt ttgttacgtt gcagaacatt gattatgctg attatgaaaa
    10081 taaccaatag agtataccta tatgactgaa gggactgact acgtgctttt aaatctttaa
    10141 accagaggga ttgcaatata ttggttaata ctaccgggag agtgtatggg caccaaagcc
    10201 acgaaaagtt tgattgaatc agcacagaga ggcgacataa aagcaatgtc tgcgttgctt
    10261 gccaccactc aggctgatgc ccgtcgctat gcccgccgac actgccatgc aagcgatgtc
    10321 gacgatgctg tacaggagtc attgctgata atttcgcgta aggtcactgc ccttaaagtc
    10381 gcagctgcct tttcctcgtg gttgtttacc gtcatcagga gggagtgcca aaaacttcag
    10441 cgaatgatgt tccggcatgt cgctctggat cacgatattg ctgaaactct cctgcaggaa
    10501 agaagcccgg actcacttcg cctagacctg gccagagctc ttaggtcttt gcctgctcat
    10561 tatctcgacg tgattctcat gcgagatttt gaagagttaa ctatagccga aatatcttcc
    10621 caactaaatg taagtgttgg tgctgttaaa agtcgtttgc atcgggcacg cgagctcatt
    10681 cgtgaatccc tgataagcac tgagggctat tagcatcact tttgagttgc tcaaagattt
    10741 attatcatga ctcgctagcc tgaatggcta gctctatgat tgtgatcata tcagatgcca
    10801 gcgccggtct gtttgacctt tccgtagttg ttcttaaaca atgaaacacc agcgacaacc
    10861 cgagtcacta cagtgatgca acaaagcgct gaaaaaccat acgctatcag cggaaaccag
    10921 ccaggaaaca gacacattaa cagtagcacc aagaccgttt cgctcccttc agtcaggccg
    10981 cctaaatagt agaacccttt gcttggatac gcggtactct ccatcccccg cttggcggcg
    11041 ataacggcaa aggcaagaaa gctgcacccg gtcccaataa agctgtaaat caaaatggcc
    11101 gcaggcaagg cgttatttgc cggtattgcc aaagcaaacc cgagtggaat ggcggagtaa
    11161 aacagaaagt ctaaagtaat atccagaaat gcaccgcgat ccgttgggcc atccaaccgg
    11221 gctacagcac catccagcaa gtcggccata cggttgaaca caatacacag cagcgcccag
    11281 taataagcat gagcaataag caatggcacc gccatcatcc cgatgccaaa gccaaaccaa
    11341 ctgatccaat cagcctttac accacggcgt tgcaatgtga ctgcgcacac ttcacgaatg
    11401 ttactgcttt ttttaagcaa ataacggtca aacatcagct ctctcctcaa gctttttcac
    11461 gcgtccatcc ggtggaatat cttgttcgtc atgagtaacc agcaacgcgg gaatgccacg
    11521 ctgttgaata tgactgaaaa caaattggcg aaaacgcccg cgtaaatgct catccaaacg
    11581 actaaaaggt tcatctaaca gcaatgcctc aggttcggcg atcagagacc gcagaacgct
    11641 caccctggcg cgttgcccac cggataacgt cgcgggatct cgctgataaa actcacccag
    11701 ttcagcatct ataaggcagt tctcaatata ttctctgcgc gaccttttac ctttcagctg
    11761 agccggtaat gccaatgcca gattttgccc tacactcaaa tgaggaaata acaggtcatc
    11821 ctgaaacaaa ataccaatac gccgcttttc tgttggcaat ccatctctcc gagtgccgtt
    11881 taaccagagg cttcctgaac cgaaaaaatc cggaggtaac tcaccaatca tccagccgag
    11941 caaggtggac ttacccgtac ccgacgcgcc catcaaggtt aatatctcgc cgggagccac
    12001 atcgaaacat tggttttgca ataagcaatt atcaccgccg tgaatagtac aatttctgac
    12061 agacaacata ttagcgtaat cctcgtcgat aatggccaac cagccggcta agcgacacag
    12121 ccataaaaaa gacgcagcct ggcagaagca gctgtagcaa agcatgaacg gctagtgccc
    12181 gcggatcgcc actgccactc agcgccacgg tttcagtcgt gaccgtggca aacctccccg
    12241 ctccggcata gagcgttggc aaatattggg caacgctgac agaaaaaccg atcgcgactg
    12301 cggcgagcag tggccgagaa agtaagggcc actttaccct taagagcaca cgccaatcgg
    12361 ataaccctaa agaacgggcg gctaccagca aacgagtgtc aagatgccga tacgccggct
    12421 ccagtatcag caacatataa ggaactaccc acaataaatg actccatacg acaccagaaa
    12481 atgttccgtc cagccctaat ctcaatagta cctcgtactg gcccatcgcc aggggtaaag
    12541 cgggcagcaa tagcggcaac cagagaaact gcccccgtcg cacatccccc cattcaagcc
    12601 aggcaaccac aataatcaaa ccacacaaac tggccacgcc acctagtgta aacgtagtga
    12661 gtaacgacgc gtaatcccct cgtgaccaac ctaccactga ccatacctca ggcaataacc
    12721 gagggtaaaa ccaactacca gccactgacc aaagcaacaa taacagcagg accagataac
    12781 cgatggttgt catcatcatc gccaacccgc tcaagagtcg tttagctgtc ccaccatacc
    12841 gtcggccggt tatgggtaat ggacaatagt gcaacaaacg ccaaaaggcg tagccgcaca
    12901 aggataatgc tgccaatatc gcagtcaaca acaggacggc gatcgtccct ttagcctgaa
    12961 tctgaatatc aggctcgttc agccattgcc atgccaacac agccagggtt ggcggattgc
    13021 caggcccaag aataatcgca gtgtccacta aggaaatgct atatgccgtc acggccaaca
    13081 gcgcccatgc cagctgcggt aacaattgcg gcaaaaggat cacgaaggcg ctgtgccatc
    13141 gtccataccc cagagtgtgg gcgatggtga gttggcgctg taaatccgtg cgtttaagta
    13201 gagctcccgc tatccatagc agaaaccagc tctctttcag cgccagtatg atacccagac
    13261 caatagcatg aggatcccca tcggccaacg agcctaaccc caatcgtgcc aaccatcccg
    13321 tcggggcaaa caggaacagg cttcccgtag caaaagcgac atgcggaacg gcaagtagca
    13381 tcggcaaacg acgttgataa cgttgccacg cttttcccgg gtaaattccg acaatcacca
    13441 ataagcaaag cagcaaagca ccgacgctgc cgatcagcga tgaagtcaac gtggcgatca
    13501 gtgcttgtcg ccattgcccg tcgagccaga atgccaacca gctatcgccg tgaaaagcgg
    13561 gggataacat caaccagagg cctggcagca agggggaaaa gatcaaactg acccccccca
    13621 tgcgatccct ctgccgaatg tagaacgtag taagggcatc aatgtacgcc gtaacgcctc
    13681 agccattccg cctcaagagc ggcggaccac cccgcattcg gctcactcaa cacggggaca
    13741 accatccctg ccgagggaga gatatcgtgc attgtccgcc gtaattctgg cggtagttta
    13801 tcgctatcca acacactagg atcgccccag taacgcggat cggctttgcg gatctgcgca
    13861 cgaggcgaca gcaggaagtt ggccaatacc tgagcgcccg ctttggcatt ggcattaact
    13921 ggaatggcga caaaatggac attccccacc atccctttac tgaaaccaaa ggtgtaggcc
    13981 gaatcaggta attgcccgtt ggcaatcagg ctggcggcgt gggcaggatt aaacgtaaga
    14041 gaaattttta acacgccatc ggccagcata cgatccatac gtgctgccga tgcgggaaaa
    14101 tctttacctt gccgccacaa cgagggatgc agttgatcga gataatgcca tagcggggca
    14161 gtgactaagg caaatgtttt aacatcggga ggttgtttta ataactgcgg tgtcgggctg
    14221 agtgacaaca acagttgttc caaaaaggcc ataccggtga agtccggtgg ccgtggataa
    14281 ctcagtcggc ctggctgctc tcgggcaaga gtcagtaatg cctgtggatc gctgacgggt
    14341 tgtgataact gccgccgatc ggcaatgaat gtcaattgtg cgcttcccca gggagactca
    14401 gccccttctg tcggaacgga aaaatcctca cgaaccggca gagtcaaatt gacatagcgc
    14461 cagttgggga gacgctctgc ccaaccgccg taaagcaacc cggcctgctt tagtgtgcta
    14521 aaattttctc cattaaccca cagtagatct atcgagcctt tttgctgccg tccagcagat
    14581 gcctcggttt ggatacgacg cacggcatct gccgtatcag ccagcggtac ccgttctact
    14641 gtgatggcat agtgttgttt catttctgtc gcaacccagc gcagatagtt attggctgca
    14701 gtatcacccc cccaggcgtt aaaatagacg gtctgtcctt gcgcctgctg ttttatttca
    14761 ttccaacggc tatccgctat cgcctgtaac gacataccaa gcgccagtat aggcacccac
    14821 attttccatc ctgccttttg caacattcca tccaccctgt tgaaattacg ttgacagtca
    14881 tagactgtgc cgttggatga atcgccggtc gagaatatct ttccataccc cggcccattt
    14941 cccttctgcg cttaaaccag cccaactcat tatggcgccg ccttgaccat cggccagcag
    15001 tgccactgct ctagattgtg gcctgaaggg cctcaatgat tcctgccgtg ctgcgcggcg
    15061 caaattttcc tccaagactg ccccttgtcg caccgcatat acgccacttt taggcgtatc
    15121 ggggagcgtt gcgcaatcac ccgttgcaaa aacatgtgga tgcgatgcac tttgtagcgt
    15181 ggtagtgatt tgaatgaatc cacgatcatc acaggataat ccggaagatt gccaccagga
    15241 tgcgggcgca ggcccgctgg ccaagagtac ccgattaccc tcccaaacaa tttgttgtcc
    15301 actcaacaga ccgtttccct ggattgcatc tattgggcaa tgttcctgca cttgcactcc
    15361 cgcccgtttc aggtggtagt gcgcacgttg gcgtaccccg ggagcatgcg agcctaacag
    15421 catatctgaa cagaacagag tcagtgacgg cgtctgattc gacatagcca gtgccagctc
    15481 cacacccgcg gcgcccccgc caattattgc cagggatccc ggatgccgct gccagtgtgc
    15541 aaggcgaatc aaaaagtcgg caaagggttt caccggcaat acgtccatta cggatgaaag
    15601 attgtgcgga gaacgcaatg ccgcgccggt attcaaggac acacattccc cctgtagtaa
    15661 acgtccatct tccaaactaa cgcggccagt ctgcgcatcc agtgctatca tcttcccctg
    15721 catcaacgtc actcctgctg cgagacaaag agggcttaat ggaatacgac actgttcgga
    15781 ctgatagcgt ccagcaagta atcccgggag catgcctgaa taccaggcat cgggcgaagg
    15841 tgacagtaaa gcaacatcgc cgaacggcgc gcccacgtca acccagtggc gtaatacctc
    15901 caaatgagca tgaccggcac caatcagcag cgtcccaaag gttttcatcg ccctaaactc
    15961 cattggtgaa aacgcgctaa ccagctcaac acgcgttgag gtgcatgatt gcgcttccac
    16021 tctccggcta catacttatt tgcttcactc agcgttgggt aaacatgaat agtgcccaat
    16081 attttattta gcccataacc ctgtttcatg gctgagacgt attcggtgat aagttcacca
    16141 gcttgctccc ccacgatagt cgcacccaaa atgcgatctt ttccaggtgc agtcagcacc
    16201 tgaatatagc cgtgggctgc atcttctgta atggccctgt ccagctcagc cagattaaaa
    16261 cacgtggttt cataggccac accggcctgt tgagcctctt gctcattaag ccctacatgc
    16321 gcgacttccg gatcggtgaa cgtggtccgc ggcataattc ggtaatccac gggaaagcgt
    16381 ttgaatgagc caaacaaggc attcaccgcg gcataccacg cctgatgtgc agccgcgtgg
    16441 gtgaactgat acggaccagt gacgtctccg actgcaaaga tatgcggaaa acgggtggcc
    16501 agaaagtcat cggtttcaag cgtaccgtcg tcccgaattt tcaacgacag tgtctccagc
    16561 ccgaagccac tcacgttggc ccggcggccc agagccagta acacccggtc gaatggcagc
    16621 acaacgtcac taccatcaac cttattacga cattccagcc attgcttatg cccttcacaa
    16681 acgaaacgct gtgcctgatg tgccaatcgc agatccacac cgtcggcccg caaactatcc
    16741 atgattactt cagcggccaa tcggtcctca cgcttgagca gacgttcacc tctgaccacc
    16801 atcgttacgg cacaccctaa acgctggaaa gcctgagcaa gttcgcagcc tatcggcccc
    16861 ccccccagaa tgagcagtcg ttgcggtttc tcacgcagca tccagatatt ttccgtcgtc
    16921 agcggcgcga tgtgttctat accgggaata acaggaataa ccggtcgagc gccggtggca
    16981 atgatcagag agcgggcact taactgccgc ccgttcaccg tgacatgcca aggtgatgtc
    17041 acggtggcag cccccgagat gacttccaca ccaagctgag tatagcgttc cactgaatca
    17101 tgtggctcta tgcgctgcac cacctgctgg actctttcca ttaccgcaga aaagtccact
    17161 tcgccagcaa catgccggaa tcccaatgcg gatgcctttg tcaggtcgtg cgccagccgg
    17221 gccgcacgga ttaatgcttt ggaaggtaca cacccggtat taagacaatc tccgcccata
    17281 ggtcctttct cgatcaatgt gacctttgct ttcactgccg ccgcgatata ggcggacact
    17341 aatccaccgg aacctgcgcc gatcacaatg atgttacggt cgaaatgacg tggttttggc
    17401 catggtttgt cacgtttacg ggcatttaaa tgatttatta ccctgcgaga aattagcggg
    17461 agtagcccca gcaagacaaa agcgccaatt atcccggggg agataatatc tgtcagcgat
    17521 tctaattgcc ccagagcccg tcctgcattg acgtacacca acgtaccggg caacatgcct
    17581 aactgactca cccaccaaaa cgttgaaaca ggcaatcgag tgagccccat tgccaaattg
    17641 attagaaaga atggaaatat cggtaccagc cgcagtgcaa acaggtagga agccccccct
    17701 tgatgaatat tcgtgtcaat tgccatcagg cgtcgggcaa aacgctgctg tatccatcca
    17761 cgtagcagga atcggctcat cagtaaagcc aatgtggcac ccaatgtaga agcaaaggaa
    17821 gccagtagag ttccgaccgt taaaccaaat agtgcaccag ccaacaaagt catcagcact
    17881 gcgccgggta aggaaagtgc tgtgatcagc acatataaca gaaagaacaa gcaccccgat
    17941 agcagcggtc tgttaaccac ccagtggttt aatgcagcct ggctattctt tattccggcc
    18001 aggctcagaa tgtggcctga tgagtaaaat agaaaaagcc cggccaaaat aaatatggca
    18061 accaataata atcccttgcc taaattcata tgctgcaccc tttgtcaaag taaacttaca
    18121 gagccttata aactgcatta aaatcaattt catagtgaaa tacaccgacc attaaaagag
    18181 ctcaatgtat aacaactttc gatttgcaaa cggccctctt aaaagtctgg ttgccgaaaa
    18241 acgggtatgc atgctttttg tgatggcagc gtaggtccag ttgctctgcg gatcggtgaa
    18301 gggtttactc agtcatgttt cgcctttgag tatcagtaaa tcagtcatgt cgtccacccc
    18361 ccctgtggcg gcgtagcggt tcacttctgg cgattaatcc cacggaatat caaccagtcg
    18421 agtgacaggt gaccggaccc gtttgttata agaaaaagag atgaagttgc ccagagggca
    18481 tgcgtcggcc aggcatcggg gtacacaaac acctcgatca ccagtgtcat gctcagaata
    18541 ccggcggcgg aaaggcgagt cgccagcccg atgacaagca ggaatgacag tatatgctcg
    18601 gcactagccg ccatgactgc cgcaatctcg ggaggcagga cgggcagacg atattcattg
    18661 cggaacaaag ccaaggtacc cgctgtaata tgcggccatc ccagttgcag gggctcgcct
    18721 cccagaatat tgatatgcag tccgtcaacc tttgtttgtc ccgacaacca gaaggtggcc
    18781 gccagtccaa gtctggcgag tatcgcaacg acctcactgg catagcgcgc agttccttgc
    18841 tgcagggagg tgcgccatcc gcggcaatgt gcagcccact tcataaagtc tctccgttaa
    18901 catcgtgtga aaaattcaaa accagtccgc gatgtatcag cccccgcaga atgagtgtgg
    18961 gttcaaaagc tgcatcacag tccagaccgt cctccagtgc cttcattagc gttttacctg
    19021 ccatcagtgc atcaacgaat cgggcactgg ccaactgtat tggctggacc ataacatcca
    19081 ggtgcgggcg aaacagcatg acactctgcg gctgagcagg atcaacaaat tcagtttcca
    19141 ttccctggtc cccgtcgcgg tcacgcatcc acaaagaaaa gatggcccag gatgagcaca
    19201 tcagctgcag agatggatgc agtacgacct taagcatcat gagttcttcc ggcagagaag
    19261 aatacaccgg tgcagccgca tccccggcat gcgaggcgcg aataaatgcc atatccatcc
    19321 gcgccaggtc gctaagccaa ggataatctc tcagcggctc aaaggccgca ataaacgccg
    19381 gcatgttgct accatagaac gccagtaccg gtgagtctgg caggtgttgc cggatatagc
    19441 aggcggacat ggcggtaaaa aaggcctccc cgacctgagt acgtatcgtc gggaaattat
    19501 ccgcaagggc ttcggtcagt gaaaccataa cgttattacg ataaattcca taacgctgtg
    19561 ccggatcgct gccattccag gtccgtaatc ctagtggtgc ttcaagttct ggcctcagta
    19621 acggatagcc gaaggctgat tcataatcag gctcagaggg atcgcaggtg atagtctgca
    19681 cgcttaacct cctccatcag tactgaaaat gcgggaatat tgctgtccca ttcaatgatc
    19741 gtggcaaccg ggccggtatg acgcagtgct gttttataaa gcgcccagac tgggtcggat
    19801 acctgacagt catgactgtc tatcagcaat ggtgccccca tactgtccag agattgggtg
    19861 tgaccagcca gatgtatttc ccccacatgc tcagtgggaa gttcctgtaa cattgtaacg
    19921 gcatcataat ggtgattaat gctggaaata tgaagattgt taatatcaag cagcaggccg
    19981 catccggtcc ggctgaggat ctcggttaaa aaagcagtct cggaccaagt cgatgcagca
    20041 aactcaacat aagtagccgg attttccaac aaaatcggac gcttcagctt ctcctggacc
    20101 tgatctatgt ggctgcacac acgcaggagt gtttctttgg tgtatggcaa gggcagtagg
    20161 tcattgagaa aatgtgtctg atgtgttgac catgccagat gctccgaaaa agcggccggc
    20221 tgatattgtt ggaccaacct ggaaacttcc tccagatggg ctctgttcag gggggcctgc
    20281 cctccaatag acatgccaac accatgaata gacaacgggt agtgtctgcg aatacgttgc
    20341 agatagtggt gaagcggtcc gcccgcaacg agatagtttt cggcatggac ctcaaaaaaa
    20401 cctatatccg gctggtcatc aattattgtt gaaaaatgct ggggtttgag gccagttcct
    20461 gcgcgcaggg ggagatgatg gtttatcgcc ctggcagggt gaccatcata aagagctggc
    20521 atcgggttta tggcagtcat tctggtggcc tccctgagtg acgggttatt atcaggacgc
    20581 tttttcggta aaggcctgta actgaccttt accggttggc gatgtggatg agtcagtttt
    20641 caggcaggta ccggtaggta ccattttgaa tgcatttccc tgataatcgt ttttggctgt
    20701 tcctgcacag gtcgtacctg cccctgcctt acagtcattt tttcctttca gggctacgcc
    20761 aaaacatttc tccatagccg gtttgtcggc ggcaaaggct gtcgtcgatg cggaagccag
    20821 tacgctcccc agcgccaggg caactacagc agcggtgtgt tttttcatgt cagtgttcct
    20881 atgaatgtca ctttgtgacg ggataaaatg gccccattaa acgtatcggg gagccttttg
    20941 actcggcagt gcctccatga aaagacactc gctgagaacc tgattactca ctgtcgacta
    21001 tccgataacg aagggggaac cgcagaattc ggaaaaaatc gtacgctaag ctaacggtgt
    21061 tctcgtgaca gctctttgac taggctttct aaggccattc tgatagccct gacttcctga
    21121 aaagccatgg ctaaaatttg tgcggctaaa agggataacc gatggtaaag taagttatcc
    21181 ctgtcgagat actgaaaagc gttatcctcg tttttcccaa aactgttttg ccagttcgct
    21241 cagagcgcta gttaactgag cgacagattt cgcactttgc aaattattct gcccggtctg
    21301 tacattggta tcagcagcat cgcgtatatt gataatactg cggtttatgt cttcacttac
    21361 tgctccctgc tgctcgaccg cagtcgctat ttgcgcgttc atgtcggtaa tttcgttaac
    21421 gcgttggcca attccatcaa gagctgtagc tgcttcctct gcgtgagcta cactcgtgtg
    21481 cgcttgccga ctactttgct ccatgactgt aacagcggat tgcgctcgct cttgtagagc
    21541 gctgatcatg ctttgaatat ccgttgtcga ttgctgtgtg cgagcagcaa gactgcgaac
    21601 ctcatcggcg acaacagcaa aaccacgccc ctgctcacca gcacgcgcgg cctcaattgc
    21661 tgcgttgagt gccaacaaat tcgtttgctc ggcgatccct cgtataacgt caagaacttt
    21721 tgatatctcg ttactttgac cttcaagctc atgaataacc tgagtggctt gcctaatttc
    21781 accttcaagg gcagtgattg actggcttgt gtgggctacc agacgctggc cagatgccgt
    21841 ctcagtgtct gctcttccgg ccgcatctgc agcatgctgt gcattgctcg caacctcttg
    21901 aatgcttgcc accatttggt ttactgccgt tgctatttga tctgtctctg cctgctgctc
    21961 aactgtaagt acattgcttg actcaatatc ctttagtagg cctcgggtgt gttcgctaag
    22021 ccgatttgat gcatcaccta tgcgacctac tatggcgcct gtttcagctt gcatcattcg
    22081 taaagcaaac tctatttggc caaactcatc ggtgcgccca gtgtagaggg attgacttaa
    22141 tgggttattg gaaatattcc tggctctttc aaccagtctt ccaagaggag agagaatagc
    22201 caaaacacta acagagctta agcttcctga cattaaagtg gctaacaata agctgcttat
    22261 tgatgtatca gtaagcatgc cggcagccat tgcgcttgat ataatactac cccatatgag
    22321 caagagtatt ttcacggaaa agctagcagc caatttcggc ctcgcggcct tcccgcttct
    22381 caattgagca tataattttt ccgcagccaa aacctgctca ggttcaggct tggtccttac
    22441 agactggtat tcaacaatcg aaccattctt agctattggc gttacataag cacttaccca
    22501 atagtggtcg ccatttttac agcgattttt tactagcccc atccatgagc ggccagattt
    22561 taatgtactc cacatatgct caaatgcagc aggcggcata tctgggtgtc ttacgatgtt
    22621 gtgaggctgg cctaatagtt cttcctcagt gaaaccactg attttaatga agtcaggatt
    22681 aacgtacgtg atatggcttt gaggggaggt agtcgaaaga atattggcat cttttgggag
    22741 ttctaagttt cgacccgtca ctggtaaatt ctggcgcata agaacctcaa gggttggctg
    22801 ttttatttta ttgttttcgg cattaagccc aatttcttga gcgttacgat aaagctagca
    22861 tggaaacgat aggtgcaagc aagttaaggg ttgcatcgcg catgtcaatc taggctatac
    22921 cctaacttga tgtcaggcag ggccgcgccg cttcgtcaga atagagtctg ctttcccatt
    22981 ttttgacaca tgcccgcgaa ggttatagat ttcagcctga cagaaatggg ctttgaggca
    23041 caacggaaca gaaagtgcac ttaagccgcc ttgtcatttt cagaagacga ctgcaccagt
    23101 tgattgggcg taatggctgt tgtgcagcca gctcctgaca gttcaatatc agaagtgatc
    23161 tgcaccaatc tcgactatgc tcaatactcg tgtgcaccaa agcgaggtga gcatggcgac
    23221 ggacacccca cggattccag aacaaggcgt ggccactctg cctgatgagg cttgggagcg
    23281 tgcgcgccgt cgtgcggaga tcatcagtcc gttggcgcag tcggagacgg tcgggcacga
    23341 agcggccgat atggcggctc aggcgctggg cttgtctcgg cgccaggtat acgttctgat
    23401 ccggcgtgcc cggcaaggca gcggcctcgt gacggatctg gtgcccggcc agtccggtgg
    23461 aggtaaaggt aaggggcgct tgccggaacc ggtcgagcgc gtcatccacg agctactgca
    23521 aaagcggttc ctgaccaagc agaagcgcag cctagcggcc tttcaccgcg aagtcactca
    23581 ggtgtgcaag gctcaaaaac tgcgagtgcc ggcgcgcaat accgtggcct tacggatcgc
    23641 tagccttgac ccgcgcaagg tcatccgccg gcgggaaggc caggatgccg ctcgtgacct
    23701 acaaggtgtg ggcggcgagc ctcctgccgt gaccgcgccg ctggagcagg tgcagataga
    23761 ccatacggtc atcgacctga tcgtggtcga tgaccgcgac cggcaaccta ttggccgccc
    23821 gtacctgacc ctcgccatcg acgtgttcac ccgctgcgtg ctcggcatgg tcgtcacgct
    23881 ggaagcgccg tctgccgttt cggttggcct gtgcctcgtg catgtcgcct gcgacaagcg
    23941 cccttggctg gaaggactga acgtggaaat ggattggcag atgagcggca agcccttgct
    24001 gctctaccta gacaacgcgg ccgagttcaa gagcgaggcc ctgcgccggg gttgcgagca
    24061 gcatggcatc cggctggact atcgcccgct gggacagccg cactatggcg gcatcgtgga
    24121 acggatcatc ggcacggcga tgcagatgat tcacgacgaa ctgccgggaa cgaccttctc
    24181 caaccctgac cagcgcggcg actacgattc cgaaaacaag gccgccctga cgctgcgcga
    24241 gctagagcgc tggctcacat tggcggtcgg cacctaccac ggttcggtgc acaacggcct
    24301 gctccaaccg ccggccgcgc gctgggccga ggccgtggcg cgtgtcggcg taccggccgt
    24361 cgtcacacgc gctacttcgt tcctggtcga ttttctgccg atcctccggc gcacgctgac
    24421 ccgcaccggc tttgtcatcg accacatcca ctactacgcc gatgcgctca agccgtggat
    24481 tgcgcggcgt gaacgctggc cgtcctttct gatccggcgc gatccgcgcg acatcagccg
    24541 tatctgggtc ctggaaccgg agggacagca ttacctggaa attccctacc gtaccttgtc
    24601 gcatccggct gtcaccctct gggaacaacg gcaggcgctg gcgaaactgc ggcagcaagg
    24661 gcgcgaacag gtggatgagt cggcgctgtt ccgcatgatc ggccagatgc gtgagattgt
    24721 gaccagcgcg cagaaggcca cacgcaaggc gcggcgtgac gcggatcgcc gccagcacct
    24781 caagacatca gctcggccgg acaagcccgt tccgccggat acggatattg ccgacccgca
    24841 ggcagacaac ttgccacccg ccaaaccgtt cgaccagatt gaggagtggt agccgtggac
    24901 gaatatccca tcatcgacct gtcccacctg ctgccggcgg cccagggctt ggcccgtctt
    24961 ccggcggacg agcgcatcca gcgccttcgc gccgaccgct ggatcggcta tccgcgcgca
    25021 gtcgaggcgc tgaaccggct ggaagccctt tatgcgtggc caaacaagca acgcatgccc
    25081 aacctgctgc tggttggccc gaccaacaat ggcaagtcga tgatcgtcga gaagttccgc
    25141 cgcacccacc cggccagctc cgacgccgac caggagcaca tcccggtgtt ggtcgtgcag
    25201 atgccgtccg agccgtccgt gatccgcttc tacgtcgcgc tgctcgccgc gatgggcgcg
    25261 ccgctgcgcc cacgcccacg gttgccggaa atggagcaac tggctctggc actgctgcgc
    25321 aaggtcggcg tgcgcatgct ggtgatcgac gagctgcaca acgtgctggc cggcaacagc
    25381 gtcaaccgcc gggaattcct caacctgctg cgcttcctcg gcaacgaact gcgcatcccg
    25441 ttggttgggg taggcacgcg cgacgcctac ctagccatcc gctccgatga ccagttggaa
    25501 aatcgcttcg agccgatgat gctgccggta tgggaggcca acgacgattg ctgctcactg
    25561 ctggccagct tcgccgcttc gctcccgctg cgccggcctt ccccaattgc cacgctggac
    25621 atggctcgct acctgctcac acgcagcgag ggcaccatag gggaactggc gcacttgctg
    25681 atggcggcgg ccatcgtcgc cgtggagagc ggcgaggaag cgatcaacca tcgcacactc
    25741 agcatggccg tttacaccgg acccagcgag cggcggcggc aattcgagcg ggaactgatg
    25801 tgaagcctgc gccgcgctgg ccgctgcatc ccgccccgaa agaaggcgag gcgctgtcct
    25861 catggctcaa ccgcgtggcc ctttgctatc acatggagga gcccgacctg ctggagcacg
    25921 atcttggtca cggccaggtc gatgacctgg acaccgcgcc accactctcg ctgctggcgt
    25981 tgctttccca gcggagcggc atcgagctgg accggctgcg ctgtatgagt ttcgccggat
    26041 gggtgccttg gctactggac agccttgatg accagattcc agacgccttg gaaacctatg
    26101 cgtttcagct ctcggtgttg ctgccaagac tccgccgtaa gacgcgatcc atcacgagct
    26161 ggcgtgcctg gctgcccagc cagccgataa accgcgcctg tccgctctgc ctgagcgatc
    26221 cggagaacca agccgtactg ctcgcgtgga agctgcccct gatgctgagc tgcccgctgc
    26281 atggctgctg gctggaatcc tattggggcg tgccagggcg gtttctcggc tgggagaacg
    26341 ccgacgccga accgcgcacc gccagcgacg cgattgcggc gatggaccag cgtacctggc
    26401 aggcactgac aaccggtcac gtggagctgc cgcgccgacg catccacgcc ggattgtggt
    26461 ttcgacttct tcgcacgctg ctcgatgagc tgaacacccc gctttccgcg tgcggaacct
    26521 gcgcggggta tccccgccaa gtctgggaag gctgcgggca tccgctgcgt gctgggcaaa
    26581 gtctgtggcg accgtatgaa accctgaatc cgatagtacg gttacagatg ctggaggcgg
    26641 cggcaacggc aatcagcttg attgaggtga gggacatcag cccgccaggc gagcaggcaa
    26701 agctattctg gtccgagccc caaaccgggt tcaccagtgg cctgccgacg aaagcgccga
    26761 agcccgagcc catcaatcac tggcagcgtg cagtccaggc catcgacgag gccatcattg
    26821 aagcgcgaca caaccccgag acggcacgct cgctgttcgc gttggcttcc tatggtcggc
    26881 gcgatcccgc ttccttggaa cggttgcgcg ccaccttcgt gaaggaaggc atcccgccgg
    26941 aatttctgtc acattacctg cctgatgcac cctttgcatg tcttaaacaa aatgacgggt
    27001 taagtgacaa attttgacgg atagagcttt ccggctcaca ctgtcacata atcgaacgta
    27061 tacgtgacgg gtgaaaaggt gctgatcggc tacatgcggg tatcgaaggc ggacggatcc
    27121 cagtccacca atttgcaacg cgatgcgctc atcgccgctg gtgtgagcct tgcgcacctt
    27181 tacgaggatc tggcctcggg caggcgcgat gatcgcccag ggttggctgc ttgcctgaag
    27241 gcgcttcgtg aaggggacac gctgatcgtg tggaagctcg atcggcttgg ccgtgatctg
    27301 cgccacctga tcaacaccgt gcacgaccta actgcgcgta gcgtgggcct gaaggtcctg
    27361 accggtcacg gtgcggcggt cgacacgacg actgccgccg gcaagcttgt gttcggtatt
    27421 tttgccgcgc tggccgagtt cgagcgtgag ttgatttccg agcgaacagt cgctggactt
    27481 atctcggcgc gcgctcgcgg caggaaaggg gggcgcccct tcaagatgac cgccgccaag
    27541 ctacgcctgg cgatggccag catggggcaa ccggaaacca aggtgggcga tctctgcgaa
    27601 gaactcggga ttacccggca gacgctctac cggcacgtgt cgcccaaggg cgaactgcgg
    27661 ccagacggcg taaagctgct ctccctcggt tcagccgcat aaatggaggc gacctggaac
    27721 ggggcgctgt tcagtgcggc aacgatccga ttaccggtgt cgacccagag cagccgtaga
    27781 gcttttggga aagctgtcgt tcaacgtttg acgtgagggg ccgccgtagc ggcgaagccg
    27841 cgaagggaac ccgcaagcgc agcttgtggg cggtccctct cgacggaatg gttagatgcg
    27901 accgttttag tgaacacttg ccttagatag caagttgagc acagcaacgc cgctgataat
    27961 gaagccgaca ccaacaaatc cccacatatc tagtttttga ccatgcaaaa cccatgcaat
    28021 cgcagtgacc aagacgatcc cgaggcccga ccaaactgcg taggcgattc caacaggaat
    28081 cgatttgagt gtcagcgaca ggaaataaaa agcagcagcg tatcccgcta cgacgataaa
    28141 agacggtact aacctagtaa agccctcact agacttgagc gcagaggttg caatgacctc
    28201 aaaaataatg gccgtagcca gaaataacca atttttcaaa atatttctcc atggagttcc
    28261 gcgaagaaat tttaggttcg atttaagaaa aaaaacagtc ttgttgctgg ccgaaatttg
    28321 tgcgcacagc aaagc
//
LOCUS       DAPGOF010000051        28253 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.051, whole genome shotgun sequence.
ACCESSION   DAPGOF010000051 DAPGOF010000000
VERSION     DAPGOF010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.051"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..356)
                     /locus_tag="RHV13_001910"
     CDS             complement(<1..356)
                     /locus_tag="RHV13_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HDV1241445.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTRE"
     gene            complement(427..2541)
                     /gene="fusA"
                     /locus_tag="RHV13_001911"
     CDS             complement(427..2541)
                     /gene="fusA"
                     /locus_tag="RHV13_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDV1241446.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2638..3108)
                     /gene="rpsG"
                     /locus_tag="RHV13_001912"
     CDS             complement(2638..3108)
                     /gene="rpsG"
                     /locus_tag="RHV13_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDV1241447.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3205..3579)
                     /gene="rpsL"
                     /locus_tag="RHV13_001913"
     CDS             complement(3205..3579)
                     /gene="rpsL"
                     /locus_tag="RHV13_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDV1241448.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3705..3992)
                     /gene="tusB"
                     /locus_tag="RHV13_001914"
     CDS             complement(3705..3992)
                     /gene="tusB"
                     /locus_tag="RHV13_001914"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HDV1241449.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIILIDYTDFVRLTVKHSSQMAW"
     gene            complement(4000..4359)
                     /gene="tusC"
                     /locus_tag="RHV13_001915"
     CDS             complement(4000..4359)
                     /gene="tusC"
                     /locus_tag="RHV13_001915"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HDV1241450.1"
                     /translation="MKRIAFVFSTVPHGTAAGREGLDALLATSALTDELAVFFIADGV
                     FQLLPGQKPDVVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4359..4745)
                     /gene="tusD"
                     /locus_tag="RHV13_001916"
     CDS             complement(4359..4745)
                     /gene="tusD"
                     /locus_tag="RHV13_001916"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HDV1241451.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4745..5467)
                     /locus_tag="RHV13_001917"
     CDS             complement(4745..5467)
                     /locus_tag="RHV13_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDV1241452.1"
                     /translation="MSRSLLTNETSELDLLDQRPFEQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5634..6446)
                     /gene="fkpA"
                     /locus_tag="RHV13_001918"
     CDS             complement(5634..6446)
                     /gene="fkpA"
                     /locus_tag="RHV13_001918"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDV1241453.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKASVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6667..6885
                     /locus_tag="RHV13_001919"
     CDS             6667..6885
                     /locus_tag="RHV13_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HDV1241454.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6934..7524)
                     /gene="slyD"
                     /locus_tag="RHV13_001920"
     CDS             complement(6934..7524)
                     /gene="slyD"
                     /locus_tag="RHV13_001920"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDV1241455.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7619..7819)
                     /locus_tag="RHV13_001921"
     CDS             complement(7619..7819)
                     /locus_tag="RHV13_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HDV1241456.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7829..9634)
                     /gene="kefB"
                     /locus_tag="RHV13_001922"
     CDS             complement(7829..9634)
                     /gene="kefB"
                     /locus_tag="RHV13_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HDV1241457.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9634..10185)
                     /gene="kefG"
                     /locus_tag="RHV13_001923"
     CDS             complement(9634..10185)
                     /gene="kefG"
                     /locus_tag="RHV13_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HDV1241458.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10316..12229
                     /locus_tag="RHV13_001924"
     CDS             10316..12229
                     /locus_tag="RHV13_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV1241459.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVNNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQADEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12229..13251
                     /locus_tag="RHV13_001925"
     CDS             12229..13251
                     /locus_tag="RHV13_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDV1241460.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PYWQRLELPDGDFVDLAWSENPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13245..13463
                     /locus_tag="RHV13_001926"
     CDS             13245..13463
                     /locus_tag="RHV13_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HDV1241461.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13517..14386
                     /locus_tag="RHV13_001927"
     CDS             13517..14386
                     /locus_tag="RHV13_001927"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HDV1241462.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14441..14845)
                     /locus_tag="RHV13_001928"
     CDS             complement(14441..14845)
                     /locus_tag="RHV13_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HDV1241463.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15147..15779
                     /gene="crp"
                     /locus_tag="RHV13_001929"
     CDS             15147..15779
                     /gene="crp"
                     /locus_tag="RHV13_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HDV1241464.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15830..17920
                     /locus_tag="RHV13_001930"
     CDS             15830..17920
                     /locus_tag="RHV13_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDV1241465.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17987..19207)
                     /gene="argD/dapC"
                     /locus_tag="RHV13_001931"
     CDS             complement(17987..19207)
                     /gene="argD/dapC"
                     /locus_tag="RHV13_001931"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HDV1241466.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     YQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQFKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19293..19856)
                     /gene="pabA"
                     /locus_tag="RHV13_001932"
     CDS             complement(19293..19856)
                     /gene="pabA"
                     /locus_tag="RHV13_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HDV1241467.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPDSLPACFEVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19888..20490)
                     /locus_tag="RHV13_001933"
     CDS             complement(19888..20490)
                     /locus_tag="RHV13_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HDV1241468.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20480..20647)
                     /locus_tag="RHV13_001934"
     CDS             complement(20480..20647)
                     /locus_tag="RHV13_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HDV1241469.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRSHYER"
     gene            complement(20752..21324)
                     /gene="ppiA"
                     /locus_tag="RHV13_001935"
                     /gene_synonym="rotA"
     CDS             complement(20752..21324)
                     /gene="ppiA"
                     /locus_tag="RHV13_001935"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HDV1241470.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21595..22776
                     /gene="tsgA"
                     /locus_tag="RHV13_001936"
     CDS             21595..22776
                     /gene="tsgA"
                     /locus_tag="RHV13_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HDV1241471.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVAKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            23038..25581
                     /gene="nirB"
                     /locus_tag="RHV13_001937"
     CDS             23038..25581
                     /gene="nirB"
                     /locus_tag="RHV13_001937"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HDV1241472.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25578..25904
                     /gene="nirD"
                     /locus_tag="RHV13_001938"
     CDS             25578..25904
                     /gene="nirD"
                     /locus_tag="RHV13_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HDV1241473.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            26030..26836
                     /gene="nirC"
                     /locus_tag="RHV13_001939"
     CDS             26030..26836
                     /gene="nirC"
                     /locus_tag="RHV13_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HDV1241474.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGAISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26855..28228
                     /gene="cobA"
                     /locus_tag="RHV13_001940"
     CDS             26855..28228
                     /gene="cobA"
                     /locus_tag="RHV13_001940"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDV1241475.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6810 a         7599 c         7166 g         6678 t
ORIGIN      
        1 tgctcacgag tctgcggcat cgggccgtca gtcgcagcaa ctaccaggat cgcgccgtcc
       61 atctgagcag caccggtgat catgttttta acatagtcgg cgtgccccgg gcagtctacg
      121 tgtgcgtagt gacgggtcgg ggtgtcatat tcaacgtgag aagtgttgat ggtgatacca
      181 cgagcttttt cttccggcgc gttatcgatc tggtcgaatg cacgagcagc accgccgtag
      241 gttttagcca ggacggtggt gattgcagcg gtcagagtag ttttaccgtg gtcaacgtgg
      301 ccgatagtac caacgttaac gtgcggtttt gtacgttcaa atttttcttt agacacggct
      361 atattcctta ctatagtgct ctccccttca ggagagagca cgggactttg gtattaaccc
      421 ttaggcttat ttaccacggg cttcaattac ggcctgagca acgttactcg gcgcttcatc
      481 atacttcagg aattccatag tgtatgatgc acgacctttg gtcagagaac gcagctgagt
      541 tgcgtatccg aacatttcag acagcggtac ttcagcgtgg atcttaacgc cagtaacttc
      601 agattcctga cctttgagca taccacgacg acggctcaag tcaccgataa cgtcaccggt
      661 gttctcttcc ggagtttcta cttcaacctt catgatcggc tcaagcagaa ctggtttcgc
      721 tttcttaaag ccttctttaa aggcgataga agcagccagt ttaaacgcca gttcagagga
      781 gtcaacgtca tggtaagaac cgaagtgcag acgaataccc atgtctacta ccgggtagcc
      841 tgccagcgga cctgctttca gctgttcctg gataccttta tcaacggccg ggatgtattc
      901 gccagggatt acaccacctt taatgtcgtt gatgaactcg tagcctttcg ggtttgaacc
      961 cggctccagc gggtacatgt cgataacaac atgaccatac tgaccacgac caccagactg
     1021 tttcgcgtgt ttaccttcaa catcggtaac tttctggcgg atagtttcac ggtaagcaac
     1081 ctgcggttta cctacgttcg cttcaacgtt gaattcacgc ttcatacggt caacgatgat
     1141 gtcgaggtgc agttcgccca tacccgcgat gatggtctgg ttagattctt cgtcagtcca
     1201 tacacggaaa gacgggtctt ctttagccag acggcccaga gccagaccca ttttttcctg
     1261 gtcagctttg gttttcggtt caactgcgat ggagattacc ggctcaggga attccatacg
     1321 ttccagaatg atcggcgcat ccgggtcaca cagggtgtca ccagtggtta cgtctttcag
     1381 accgatagca gcagcgatgt cgcccgcgcg aacttctttg atctcttcac gtttgttagc
     1441 gtgcatctga acgatacgac cgaaacgctc acgtgcagct ttcacggagt tcagtacggt
     1501 atcaccagag ttaaccacac cggagtaaac acggaagaag gtcaggttac ctacaaacgg
     1561 gtcggtagcg attttgaacg ccagtgcaga gaacggctcg tcatcacttg cgtgacgttc
     1621 agccggagta tctttaccgt cgtccaggat accgttgatc gcaggtacgt caaccgggga
     1681 tggcaggtaa tcaattaccg catccagcat cgcctgaaca cctttgttct tgaacgcaga
     1741 accacaggtt accaggatga tttcgttgtt cagaacgcgc tgacgcagag cacctttgat
     1801 ttctgcttca gtcagttctt caccacccag gtatttttcc atcagctctt cagaagcttc
     1861 agctgcggat tcgatcaggt tctggtgcca ttcgttagcc agttcaacca tgtctgccgg
     1921 gatatcttcg tattcgaagg ttacgccctg gtcagcgtcg ttccagttga tagctttcat
     1981 tttcaccagg tcaacaacac cggtgaaatg ttcttcagca ccaatcgcca gctgcagcgg
     2041 aaccgggttc gcgcccagac gggttttgat ctggttaaca actttcagga agttcgcacc
     2101 catgcggtcc attttgttaa cgaacgcaat gcgcggaact ttatatttgt ttgcctgacg
     2161 ccatacggtt tcagactgcg gctgaacacc accaactgcg cagtaaacca ttaccgcacc
     2221 atcgagaaca cgcatggaac gttctacttc gattgtgaag tcaacgtgcc ccggggtgtc
     2281 gatgatgttg atgcgatgcg gctcatactg cttagccata ccagaccaga atgcagtagt
     2341 cgcagcggaa gtgatggtaa taccacgttc ctgctcctgc tccatccagt ccatggttgc
     2401 agcgccgtca tgaacttcac cgattttatg gtttacaccg gtgtagaaca gaatacgttc
     2461 ggtagtagtg gttttaccgg cgtcgatgtg cgcactgata ccgatgttac ggtagcgtgc
     2521 gatgggtgtt gtacgagcca tttgtttcct cgtttatctt ttaggcgttc aatttaagta
     2581 gcccaaagcg ggctgcttac tggaagcgcc cgcctggtga ctaaaactcc gaagggatta
     2641 ccaacggtag tgtgcgaacg ccttgttggc ttcggccata cggtgaacgt cttcacgttt
     2701 cttaactgca gtacctttgt tttctgcagc atcagaaagt tcgttcgcca ggcgcagagc
     2761 catggattta tcaccgcgtt tacgagcagc ttcaacgatc caacgcattg ccagagcatt
     2821 acgacgaacc ggacggactt caactggtac ctgataagta gaaccaccaa cgcggcgaga
     2881 cttaacttct acagtcgggc gcacgttttc gagagctact tcgaatgctt ccagttcaga
     2941 tttaccagag cgctgagcca gggtctccag cgcgctgtat acgatagatt cagcagtaga
     3001 ttttttacca tctaccatca ggatatttac aaatttagcc agcagttctg atccgaactt
     3061 cggatccggc agaattttac gctgaccaat gacgcgacga cgtggcatgg aaatactccg
     3121 ttgttaattc aggattgtcc aaaactctac gagtttagtt tgacatttaa gttaaaacgt
     3181 ttggccttac ttaacggaga accattaagc cttaggacgc ttcacgccat acttggaacg
     3241 agcctgctta cggtctttaa cgccggagca gtcaagcgca ccacgtacgg tgtggtaacg
     3301 aacacccggg aggtctttaa cacgaccgcc acggatcagg atcacggagt gctcctgcag
     3361 gttgtgacct tcaccaccga tgtaggaagt cacttcgaaa ccgttagtca gacgaacacg
     3421 gcatacttta cgcagcgcgg agttcggttt tttaggagtg gtagtatata cacgagtaca
     3481 tacgccacgt ttttgcgggc atgcttccag cgcaggcacg ttgcttttcg caactttgcg
     3541 agcacgtggt ttgcgtacca gctggttaac tgttgccatt aaatagctcc tggttttagc
     3601 ttttgcttcg taaacacgta ataaaacgtc ctcacacaat atgaggacgc cgaattttag
     3661 ggcgatgccg aaaaggtgtc aagaaatata caacgatccc gccatcacca ggccatctgg
     3721 ctggagtgct taaccgtaag tctgacgaaa tcagtatagt caatgagaat gatgtcgttc
     3781 gaaatttgac cagtcaaacc gcgggcaata aggtcttcgt tcagggcata gaccttaatg
     3841 ggggcattac gcagactttc aaggtagcgg ttaccgtcaa ctgcggcagt tacgccatct
     3901 tgcaataata gcagctcgtc gccttcactg agcagacgca gcagcgcagc aaaatccgtc
     3961 agccagggtg aacgatgtaa tgtgtgcagc ataaacgcct caaaacctca aaataacatc
     4021 gtagttggcg agttcgcggc gtaaggcatc tgcttcgagc ggcgtggctt cgacaacaaa
     4081 gggggtctgc ggatctaacc cgcgttcgcg cagtgaagcc gcacaaaccc agcactgttc
     4141 aatgtcgtac agacccaaca atttaaaagt ggcaatgtaa tcacgcgcca gcactacatc
     4201 gggcttttgt cctggcagta gctgaaaaac gccatcagca ataaagaaga cggccagctc
     4261 gtcagttaac gcggaagttg ccagtaaagc atctaaacct tcccgcccgg ccgcggtacc
     4321 atgaggcaca gtagaaaaaa caaacgcaat tcgtttcatc agaactgtac caccctgtca
     4381 caggtcagcg aggcttccgc cagcgcgcca agtccgctta aggtaaatcc cggctgaagg
     4441 tttgacgaag ccagccccag tcttccggcc tccgtttcat caacaacgcc acggcgtaat
     4501 gctgccgcta cgcagatatt cagcgccaca ccatgttgcg cattcaactg ttgccagccg
     4561 cgcacaaggt caaattcgtc gcttgccggt gaggtcagtt ggttagcgtt atagacccct
     4621 tcccgataga aaaagacgct gcttaactta tggccttctg ctatcagcgc ctgcgcaaac
     4681 tgaaaagcac tactcgcctg ttgcgtaccg tatgctggcc cggtcaccac gatggcaaaa
     4741 cgcattactt atcttgcccc tggaaatcac cgctcttgaa ctggcggatg tagagataga
     4801 cagtgtgttt ggagatgttc aggcggtcag caacctggtt gatcgcatct ttaatatcga
     4861 agatcccttt ctcgtagaga ttcagcacga tctgacgatt tttggcgtta ttagaaacat
     4921 tgcgatcggc attcacttct tcgatggtga actccagcgt ttgggtaacc agatcttcaa
     4981 cagaagaggc aaagttgacg cttgaaccga catccggggt ttctggcggc acaaaggtgc
     5041 tcataatctg cgagaaggga acatcaagat tcatattgat gcacagcaga ccaattacac
     5101 gctgttcgcg gttacgaatc gcgatagtca gggacttcat taatacgccg cttttggcgc
     5161 gagtaaagta gcatttagaa acgctgctat ccgctcccgt catatcgtgc agcatacgta
     5221 gcgccaggtc agtaattggc gaaccaatct tccggcctgt atgttcaccg ttagcaatgc
     5281 gaatggctga acattttaga tcctgcaaag agtgcaaaac gatttcacag tgggagccaa
     5341 taagcatcgc taacccgtcc accaccgctt cgtaggattt cagaatatca aaatcggtct
     5401 gctcgaaagg acgttgatcc agtaaatcca actcactggt ttcgttggtt aaaagcgacc
     5461 tggacatgaa aaaaaacact ccttttcagg agcctgtcgt taacttttca gggcaggctc
     5521 attaatgatg cgggtaacta aattaataca gcggaggttc cgctttccag cactaattat
     5581 atccggcctg taataaaaaa accgccgcct ggtcaggcgg cggttcttaa tgcttatttt
     5641 ttagcagaat ctgcggcttt cgcatcagct tccggctttg catcagcctt cggcgctggt
     5701 ttcacatcca gcagctctac gtcaaacacc agggtagaat tcggtgggat ccccggtaca
     5761 ctcgctttgc cgtaagccag ttctggtgga ataaccagtt tgatcttacc gcctttcttg
     5821 atgttcttca gaccttctgt ccaacctggg ataacaccgt ccagacgaaa agagagcggt
     5881 tcaccacggg tgtaagagtt gtcgaactct ttaccgtcga tcagcgtacc tttgtagttc
     5941 actacaacag tatcgctgtc tttcggtgct tcgcctttac cggcttctac tacctgataa
     6001 accagaccag ttgaagaggt tttcacacct ttctctttgg caaatttctc gcggtactct
     6061 ttaccttttg cttcgttatc agccgcgtct ttttccatct tcgcctgagc agaagacttc
     6121 acgcgagctt cgaatgcttg cagagtctgt tcgatctctt gatcggagag tttgctctta
     6181 tcagcaaatg catcctgaac accagcgatc agctgatctt tatccagttt gatgcccagt
     6241 ttttcttgtt cttttagaga gttttccatg taacgaccca gcgaggcacc cagtgcataa
     6301 gctgatttct gatcgtcatt tttgaacgct gctttgctgt cagcagttgt agcaggtttt
     6361 gcagcttcag cagcaaaagt gattggtgca tgcagggcaa cggccattgt ggtcgccagc
     6421 agcgttactt taaacagtga tttcatccat atctccagga tcggggcatc tcaccccagg
     6481 gttaactatt atcagaaggg tactataaag cgttgtcgaa caaatctaca tacagacacg
     6541 ccctattatc atctattttc agactctttt tgtttaaatt agtttcgatg accgcgaaat
     6601 gagtgctgtc tcgggcagca aagttaagta gaatccgcgg caaccattcg acaaaagagg
     6661 tgaatcatgc aggatttatc attggaagca cgcctggcag agctggagag ccgactggct
     6721 tttcaggaaa tcaccattga agaactgaac gtcacggtga ccgctcatga aatggagatg
     6781 gcgaaactgc gcgatcatct gcgtctgctg accgagaagt taaaagccag ccagccgtcg
     6841 aacattgcgt cgcaggctga agaaacgcca ccgccacatt attgaggcgt aaaaaaagcg
     6901 gggattcccc gcttttttgt caccttttcg gtattagtgg caaccgcaac cgccgttgcc
     6961 tttaccgcca cagcagcctt cgccaccgtg ttcatgaccg tgatcgtggc catgaccgcc
     7021 gcagcaaccg tcgtggtcgt gatcgtggtg atgatcgtgc gcgccgtgaa cgtgaccatg
     7081 agccagttct tcttcggtcg cttcacgaat cgccacaacc tcaacgttga atttcaggtt
     7141 ctgaccggcc agcatgtggt taccatcaac cactacgtga tcgtcttcaa ccgcagtgat
     7201 ttcaaccggt accggaccct ggtcggtttc agccaggaaa cgcataccta cctgcagttc
     7261 atcaacgccc ataaatacgt ctttaggaac acgttgcacc aggttttcgt cgtactgacc
     7321 gtaagcgtcg ttcgcgccaa cagcgacatc aaatttgtcg ccaacttcat gaccttccag
     7381 cgccgtttcc aggccagaga tcagggaacc gtgaccatgc aggtagtcca gcggcgcact
     7441 caccggagac tcatcaacca acacaccgtc ttctgtacgt acctgatagg ccaggctgac
     7501 caccaggtct tttgctactt tcatgatatc tcctgagcat gggaagaata gtggcgcaga
     7561 ttgtagcgga attctgcagc cgtgtactca ctagcttaaa aaaactcggc gcatatcgct
     7621 agtccggatg aaaaatcccg atcacttgct catctttgcg aacgtgatcg cgggcttctt
     7681 tgtctgcttc tcgcatctgg tgtccgcact taacgcattc aacaatatca atattatttt
     7741 cgcgccacat cgccattgaa tcctgcgcct gacaggccgg gcattttgcg cccgcaataa
     7801 aacgttttcg gattgccatc tttaccctct actcaaattc atcccagcca tccagctggc
     7861 gtcgttcttg ttgcatttca cgctggaaaa tctcttccag ttcgcgtcgg gcttccctgg
     7921 cgcgagaaat ttgtacggta tcggcatgca ttgggatgag ctctcgcagc atccgcatat
     7981 ccaggcggcg aaaatgcagt tgcgcccgct gtgcctgatg cggatgcatg ccaagcgtga
     8041 ccagcgtctt gcgccccagc tctaacgcac tggagaatgt ttcacgggaa aactgcgtca
     8101 cccctgcctg taataactca tgcgcttcca cacgtccgcg cgctcgcgca agaatatgca
     8161 aatgcggaaa gtgctgctgg catatttcca ccagcttcat ggtgtcttcc ggctcgttac
     8221 aggtaatgac gatagactca gcggcctctg cacccgcaga acgtaaaaga tctacctgcg
     8281 tggcgtcgcc gtaataaact ttgtagccat atttgcgcat caggttaacg gcgctgatat
     8341 cccgctccag cacagtaatg cgcattttat ttgccatcaa caaacgacca atcacctgcc
     8401 caaaacgtcc aaagcccacg acaatgacct ggggtttatc atcgttgacc cacggttttt
     8461 cgtcttcttc ttccggtccg ttaaactggc gggatagcca tttatccacc agcttcatca
     8521 gcagcggcgt ggtcatcatg gaaagcgtca ccgtcaccag caacagcgcc atctggtcgc
     8581 cctggaataa gcgttgtgaa gaagcggtag aaaagaggac aaaggcaaac tcaccgccct
     8641 gactcaacac gccagcaaac tgcatccgct ctgagctacg cacgccatac aatcgcgcca
     8701 gcagatacag cacgagaatt ttcaccgcca ccagcacaac cacgcttatc actacccaca
     8761 acagatgggt ataaagcacc ccgaggttga gcgacatgcc gacagagata aagaacaaac
     8821 cgagcagcaa gcctttgaag ggatcgatag ccgtttccag ttcatggcga tattcacttt
     8881 ccgccagcag cacgcccgca ataaatgtac cgagcgccat cgacagcccc aacgcatcca
     8941 taaacaatgc ggaacccaat accagcagca gcgtcgcggc ggtgaacact tcccgcacgc
     9001 cagaagctgc aataaaacgg aataccggac gcaataaata gcgcccgcca atcagcatgc
     9061 cgacaaacgc cagcaccttc atgccaatct tcatccagtc gaaatgttcg tctgccgaac
     9121 ccgccaacaa cggcactaac gccagtgccg ggatcaccgc cagatcctga aacagcagta
     9181 ccgaaaaccc gagctgcccg gattcgctgc gattcatccc tttctcacgc atcaattgca
     9241 acgccattgc agttgaagac atcgcaaggc caatgccacc gacaaccgcc gcctgccagg
     9301 cgaaatccgt cagcatcaat aatcccgcca gcaacgccgc gcttaacagc acctgtgccg
     9361 cgcctacgcc aaaaatcgaa cgccgcagtt gccaaagttt ggagggattc aactcaagac
     9421 cgatgataaa catcaggaat accacgccga gttccgaaaa gtggaggatc tcatcgacgt
     9481 cgctaataaa ccccagcccc cacgggccaa ttgcaatccc tgccagcaaa tatcccaaca
     9541 cagcgccaat acccagccgc gatgccaacg gcaccgcagc caccgccgcg aagagaaaca
     9601 gcactcctgc aagtaaaaaa tcggaacctt ccatcagcgg cctcctggag acagcggatt
     9661 tgccagccag tcaccgtagg ctctggcgtg gctcgccagc tcctgtgcgc tttgccgtct
     9721 cgcccagtaa ataatgatgg gacttaacca atgcatccgg cacatgcccg ccgccagttc
     9781 aaaggggcgc aggacatcgc tcatcgggta gcgattcaac gcgtcataac ggtaagcact
     9841 ttccggctcg ccggtggtaa tcacactacg ccagtacttt cccgccagtt ggtttcctcc
     9901 cggcccgctg gcaaaaccac ggcttaatac ccggtccagc cactctttca gtagcgccgg
     9961 gcagctatag gtataaagag gatgctgaaa gacaatcacc tcgtgctcgc gcagtaatgc
    10021 ctgctcacgg gggatatcaa taaaaaaatc gggatagtgc gcgtaaaggt cgtgcacggt
    10081 aacattgctg agctgcgtgg ccggtttaag cagtacccgg tttgccaccg agtcctgaga
    10141 ttccggatgg gcatacagca gcaaaacttt cgctggctga gacatcatcc ccctcccggt
    10201 atggtttttg tgtatagtcg ctgttttggg ctaccattgc gcccggtgcg gcagctcgcc
    10261 catacattac attatcataa tgataagtta acatagtctg aacatacggc accttatgat
    10321 tgttttctcc tcgttacaaa ttcgtcgcgg cgtgcgcgtc ctgctggata atgccaccgc
    10381 caccatcaac cctgggcaga aagtcggcct ggtgggtaaa aacggctgtg gtaaatctac
    10441 cctgctggca ttgctgaaaa atgaaatcag cgccgacggc ggcagctaca cctttccggg
    10501 aagctggcaa ctggcgtggg tgaatcagga aacgccggcg ttaccgcaag cggcgctgga
    10561 atatgtcatt gacggcgacc gtgaatatcg tcaactagaa gcgcagctac acgacgccaa
    10621 cgaacgtaac gacgggcacg ccattgcgac cattcatggc aagctggatg ctattgacgc
    10681 atggagtatt cgctcccgtg ccgccagcct gctgcacggc ctcggtttca gcaatgaaca
    10741 actggagcgc ccggtaagtg atttttccgg gggctggcgt atgcgtctta accttgccca
    10801 ggcgctgatt tgccgttcag acttgctgct gctcgacgaa ccgactaacc acctcgatct
    10861 cgatgccgtt atctggctgg aaaaatggct gaagagctat cagggcacgc tgatcctgat
    10921 ctctcacgac cgcgacttcc tcgatccgat cgtcgataaa attattcata tcgaacaaca
    10981 aagcatgttc gagtacaccg gcaactacag ttcgtttgaa gtacagcgcg ccacccgtct
    11041 ggcgcagcaa caagcgatgt acgaaagcca gcaggaacgc gtagcgcatc tgcaaagtta
    11101 tatcgaccgt ttccgtgcca aagccaccaa agcgaagcag gcccagagcc gcattaagat
    11161 gctcgaacgt atggagctga ttgccccggc gcacgtcaac aatccgttcc gctttagctt
    11221 ccgcgcgccg gaaagtctgc caaatccgtt actgaagatg gaaaaagtca gcgcgggcta
    11281 tggcgatcgc attattctcg actcaattaa attgaatctg gtccccggct cgcgcattgg
    11341 tctgttaggc cgcaacggcg cgggtaaatc gacattaatc aaactgttag ctggcgaact
    11401 tgcgcctgtc agcggtgaaa ttggtctggc gaaagggatc aagctcggct acttcgccca
    11461 gcatcaactt gaatacctgc gtgccgacga atcgcctatt caacatctgg cacgtttagc
    11521 gccgcaggag ctggaacaaa aactgcgtga ctacctcggc ggctttggtt tccagggcga
    11581 taaagtaacc gaagaaacgc gccgcttctc aggtggggaa aaagcccgcc tggtgctggc
    11641 attaattgtc tggcagcggc cgaatctgct gctgctcgac gaaccgacta accaccttga
    11701 cctcgacatg cgtcaggcac tcaccgaagc attaatcgag tttgaaggcg cgctggttgt
    11761 cgtctcgcac gaccgtcatt tgctgcgttc caccactgac gatctctacc tggttcacga
    11821 tcgtaaagtc gaaccgttcg acggcgatct ggaagattat caacagtggt tgagcgacgt
    11881 acaaaaacag gaaaaccagg ccgacgaagc gccaaaagag aacgcgaaca gcgcccaggc
    11941 acgtaaagat cagaagcgtc gggaagcgga gctgcgtgcg caaacccagc cactgcgtaa
    12001 agagattgcc cgtctggaaa aagagatgga gaagctgaac gcgcaactgg cgcaggcaga
    12061 agagaaactc ggcgacagcg aactgtatga ccagagccgt aaagcggagt tgaccgcctg
    12121 cctgcaacag caagccagcg ccaaatccgg cctggaagag tgcgaaatgg catggctgga
    12181 agcccaggag cagcttgagc agatgttgct ggaaggccaa agcaactgat ggcgcagata
    12241 acgacgaccg atgccaatga attcagcagc agtgctgaat tcacccctat gcgcggcttt
    12301 agcaattgtc atctgcaaac catgctgccg cgtctgtttc gtcgcaaggt gaaattcacc
    12361 ccgtactggc agcggctgga gttgcccgac ggcgattttg tcgatctcgc gtggagtgaa
    12421 aaccctgcac aggcgcggca taaaccgcgt ttagtggtat ttcacgggct ggaaggcagt
    12481 ctcaatagcc cttacgccca cggtctggtt gaggcggcgc aaaagcgcgg ctggctgggt
    12541 gtggtgatgc attttcgcgg atgcagcggt gaaccaaacc gtatgcaccg catttaccat
    12601 tcgggcgaaa ccgaagacgc cagttggttt ttacgctggc tgcaacgcga atttggtcat
    12661 gcgccaacgg ctgccgtcgg ctattcgctc ggcggtaata tgctggcctg tttgctggca
    12721 aaagaaggca atgatctccc ggttgatgcg gcggtgattg tctctgcgcc gtttatgctg
    12781 gaagcctgta gctatcatat ggaaaagggc ttttcccgcg tttatcagcg ttacttgctg
    12841 aacctgttaa aagccaatgc cgcgcgcaag ctggcagcct accccggaac actgccgatt
    12901 aatctcgcgc agttaaaatc ggtacgtcgc atccgtgaat ttgacgatct catcaccgcc
    12961 agaattcacg gctacgctga cgctatcgac tattatcgtc agtgtagcgc catgccgatg
    13021 ctgaaccgga tcgccaaacc gacgctgatt attcacgcta aagacgatcc gtttatggat
    13081 catcaggtga tcccgaaacc ggaaagtctc cccccgcagg tggagtatca actgactgaa
    13141 catggcggtc atgttggctt tattggcggt acattacttc atccgcaaat gtggctggag
    13201 tcacgcattc ctgactggtt aacaacgtat ctggaggcga aatcatgttg attccgtggc
    13261 aagacctctc ccccgaaacg ctggaaaatt taattgaaag ctttgtgtta cgtgaaggca
    13321 ccgattatgg tgaacatgag cgtacacttg aacagaaagt cgccgacgtc aaacgccagc
    13381 tacagtgcgg agaagcggtg ctggtatggt cagagctgca cgaaacggtc aatatcatgc
    13441 cgcgcagtca gtttcgcgaa taacctgcaa ccgtcggcgg acgagataat aatgatagct
    13501 aagtcatgga gttactatgt ctgccaaaca tccggtcatt gcggtaacag gatccagcgg
    13561 cgcggggacc accaccacca gcctcgcgtt tcgtaaaata ttcgcccagt taaatctgca
    13621 tgcagccgag gtggaaggcg acagttttca ccgttacacc cgcccggaaa tggacatggc
    13681 gatccgcaaa gcgcgcgacg ccgggcggca tatcagctac ttcggccccg aagcgaacga
    13741 cttcggcctg ctggaacaaa ccttcattga atacggtcag agcggcaaag ggaaatctcg
    13801 caaatatctg catacctacg atgaagccgt accgtggaat caggtaccgg gaacattcac
    13861 cccctggcaa cctttaccag aacccactga tgtactgttt tatgaaggtt tacacggcgg
    13921 cgtagtcacg ccacagcata acgttgcgca gcatgtggac ttactggtcg gcgtggtgcc
    13981 tatcgttaac cttgagtgga ttcaaaaact gatccgcgac accagcgagc gcgggcactc
    14041 acgagaagca gtgatggact cagtagtgcg ttcaatggaa gactatatca actacatcac
    14101 accgcagttt tcccgcaccc atcttaactt ccagcgcgtt cccaccgtcg acacttcaaa
    14161 cccgttcgcg gcaaaaggta tcccgtcgct ggatgaaagc tttgtggtga tccattttcg
    14221 taatctggaa gggatcgatt tcccctggct gctggcgatg ttgcaaggct cattcatttc
    14281 ccacatcaat acgttagtgg tgcccggcgg caaaatgggt ctggcaatgg aattaattat
    14341 gctgccgctg gtgcaacgat tgatggaagg aaagaaaatc gagtaactct gctattacgc
    14401 cggataaaat actatccggc ttcacaacgg gatagttaaa tcacgcggca accacttcat
    14461 acgagtgagt aatattcacc gctttttcca gcatcaacgc cactgaacaa tatttctcgg
    14521 cagagagatc aaccgcacgc gcaaccgctg cgtctttcag gtcgcgaccg gtgacgataa
    14581 aatgcagatt aatgtgcgta aacaggcgtg gtgcctcttc gcggcgttca gaggtcaatt
    14641 ttacttcaca atcgaccaca tcctgacgcc ctttttgcag gatcgaaacc acatcgatgg
    14701 cactgcaacc acccgccgcc atcaacacca tttccatcgg acttggtgct ttatcgcctg
    14761 agttgccgtc cattaaaatc tgatgaccag aggcggattc gcccagaaaa gttaaccctt
    14821 cgacccactt cactcgcgct tgcatttttg ctactccact gcgtcaattt tcctgacaga
    14881 gtacgcgtac taaccaaatc gcgcaacgga aggcgacctg ggtcatgctg aagcgagaca
    14941 ccaggagaca caaagcgaaa gctatgctaa aacagtcagg atgctacagt aatacattga
    15001 tgtactgcat gtatgcaaag gacgtcacat taccgtgcag tacagttgat agccccttcc
    15061 caggtagcgg gaagcatatt tcggcaatcc agagacagcg gcgttatctg gctctggaga
    15121 aagcttataa cagaggataa ccgcgcatgg tgcttggcaa accgcaaaca gacccgactc
    15181 tcgaatggtt cttgtctcat tgccacattc ataagtaccc atccaagagc acgcttattc
    15241 accagggtga aaaagcggaa acgctgtact acatcgttaa aggctctgtg gcagtgctga
    15301 tcaaagacga agagggtaaa gaaatgatcc tctcctatct gaatcagggt gattttattg
    15361 gcgaactggg cctgtttgaa gagggccagg aacgtagcgc atgggtacgt gcgaaaaccg
    15421 cctgtgaagt ggctgaaatt tcgtacaaaa aatttcgcca attgattcag gtaaacccgg
    15481 acattctgat gcgtctgtct gcacagatgg cgcgtcgtct gcaagtcact tcagagaaag
    15541 tgggcaacct ggcgttcctc gacgtgacgg gccgcattgc acagactctg ctcaatctgg
    15601 caaaacaacc agacgctatg actcacccgg acggtatgca aatcaaaatt acccgtcagg
    15661 aaatcggtca gattgtcggc tgttctcgtg aaaccgtggg acgcattctg aagatgcttg
    15721 aagatcagaa cctgatctcc gcacacggta aaaccatcgt cgtttacggc actcgttaat
    15781 cccgtcggag tggcgcgtta cctggtagcg cgccattttg tttcccccga tgtggcgcag
    15841 actgatttat caccccgata ttaactatgc actgcgacaa acgctggtgc tatgtttgcc
    15901 cgtggccgtt gggttaatgc ttggcgaatt acgattcggt ctgctcttct ctctcgttcc
    15961 tgcctgttgc aatattgcgg gccttgatac gcctcataaa cgttttttca aacgcttaat
    16021 cattggtgcg tcgctgtttg ccacctgtag cttactgaca cagctgctac tggcaaaaga
    16081 tgttcccctg ccctttttgc tgaccggatt aacgctggta cttggcgtca ctgctgagtt
    16141 ggggccattg cacgcaaaat tgcttcccgc atcgctgctg gcagccattt ttaccctcag
    16201 tctggcggga tacatgccgg tctgggaacc gttgctcatc tatgcgttgg gcactctctg
    16261 gtacggattg tttaactggt tttggttctg gatctggcgc gaacaaccgc tgcgcgagtc
    16321 actaagtctg ctgtaccgtg aactggcaga ttattgtgaa gccaaataca gcctgcttac
    16381 ccagcacacc gaccctgaaa aagcgctgcc gccgctgctg gtgcgccagc aaaaagcggt
    16441 cgatctaatt acccagtgct atcagcaaat gcatatgctt tccgcgcaaa ataatactga
    16501 ctacaagcgg atgctgcgta ttttccagga ggcgctggat ttacaggaac atatttcggt
    16561 cagtttgcat cagccggaag aggtgcaaaa gctggtcgag cgtagccatg cggaagaagt
    16621 tatccgctgg aatgcgcaaa ccgtcgccgc tcgcctgcgc gtgctggctg atgacattct
    16681 ttaccatcgc ctgccgacgc gttttacgat ggaaaagcaa attggcgcac tggaaaaaat
    16741 cgcccgccag catccggata atccggttgg gcaattctgc tattggcact tcagccgcat
    16801 cgcccgcgtg ctgcgcaccc aaaaaccgct ctatgcgcgt gacttactgg ccgataaaca
    16861 gcggcgaatg ccgttacttc cggcgctgaa aagttatctg tcactaaagt ctccggcgct
    16921 acgcaatgcc ggacgactca gtgtgatgtt aagcgttgcc agcctgatgg gcaccgcgct
    16981 gcatctgccg aagtcgtact ggatcctgat gacggtattg ctggtgacgc aaaatggcta
    17041 tggtgcaacc cgtctgagga ttgtgaatcg ctccgtggga accgtggtcg ggttaatcat
    17101 tgcgggcgtg gcgctgcact ttaaaattcc cgaaggttac accctgacct tgatgctgat
    17161 taccaccctt gccagctacc tgatattgcg caaaaactac ggctgggcga cggtcggttt
    17221 tactattacc gcagtgtata ccctgcaact gttgtggctg aacggtgagc agtacatcct
    17281 tccgcgtctt atcgatacca ttattggttg tttaattgct ttcggtggta ccgtctggct
    17341 gtggccgcag tggcagagcg ggttattgcg taaaaacgcc catgacgctt tagaagccta
    17401 tcaggaagcg attcgcttga ttctgagcga ggatccgcaa ccaacgccac tggcctggca
    17461 gcgaatgcgg gtaaatcagg cgcataacac tctgtataac tcattgaatc aggcgatgca
    17521 ggaaccagcg ttcaacagcc attatctggc agatatgaaa ctgtgggtaa cgcacagtca
    17581 gtttattgtt gaacatatta atgccatgac cacgctggcg cgggaacacc gggcattgcc
    17641 acctgaactg gcacaagagt atttacagtc ttgtgaaatc gccattcagc gttgtcagca
    17701 gcgactggag tatgacgaac cgggtagttc tggcgatgcc aatatcatgg atgcgccgga
    17761 gatgcagccg cacgaaggcg cggcaggtac gctggagcag catttacagc gggttattgg
    17821 tcatctgaac accatgcaca ccatttcgtc gatggcatgg cgtcagcgac cgcatcacgg
    17881 gatttggctg agtcgcaagt tgcgggattc gaaggcgtaa tgcaggctaa accatcggat
    17941 aaggtgttca cgccgcatcc gacatctttt gcctgatgcg tcttatttac gccccaacca
    18001 ccttcgccac cgcgtgggcg aaacgttgca tcccttcatc aatatccgca tcttccacca
    18061 ccagcgacgg cgcaaaacgc atcacatccg gtccggcatt cagcaccatt acgccagcct
    18121 ctgcgcccgc atacaggaaa tcacgcgccc gacctttgaa ctgtggctta agctccgcgc
    18181 caatcaacag ccccataccg cgaatatcgc taaacacatc gtactgctga tcgatcttct
    18241 gcaaatggtc aacaaaacgc tggcgtttcg cctgaatgcc ttccagcact tcaggggtat
    18301 tgatgatatc aaacgccgcg cccgctactg cacaggccag aggattaccg ccgtaggtgg
    18361 aaccgtgaga accaggatga aacgcagaag caatttccgc cgtggtcagc atggcgctaa
    18421 tcgggaagcc gccgcctaac gctttcgcag aggtcagaat atccggcgtc acgccgtagt
    18481 gcatgtaagc aaacaaatca ccggtccgcc ccatcccgca ctgcacttca tcaaacacca
    18541 ataatgcctg atattgatcg cacagctcgc gcaagccctg caaaaactct ggcgtcgcag
    18601 ccgtcacgcc gccctcgccc tggatcggct caaccaccac cgcacaggtg tgatcatcca
    18661 tcaccgcttt cactgcgtgg agatcgttaa agggaacgtg gatgatgtct gccggtttcg
    18721 gcccaaagcc gtcggaatat tttggctgcc cacccaccga aacggtaaac agcgagcgac
    18781 catgaaaagc gttatggaag gcaataattt tggttttgaa cgggctatga cgcacgcagg
    18841 cgtaatggcg tgccagttta aaggcggttt cgttagcttc cgtgccggag ttcataaaca
    18901 ccacgcgttc ggcaaacgtc gcctcaatca gtttacgccc aagacgcagc gccggttcat
    18961 tggtgaaaac gttactgata tgccacagag tttcgccctg ggtttttaac gcgttcacca
    19021 gcgcaggatg gcaatggccc aacgccgtaa ctgcaatgcc acccgcgaaa tcgacatact
    19081 ccttgccttg ctgatcccag attcggctgc cctgaccttt taccggaata aactctgccg
    19141 gagcataaat cggcaggatc acttcatcga aagtcgcgcg tgtaattgct gtttgttcaa
    19201 ttgccatctc atgatcaccc tgttacgcat aaataaatgt gaaattataa tcacaaaata
    19261 tgcataaaaa atcattgaat ggcaatcaga aatcagcgat gcaggaaatt agccagcagt
    19321 tgatgtcctt gttcgctaag aatactttct ggatggaact gcacaccttc cagatcccac
    19381 tggcgatggc gaatccccat aatctcgcgg gtttcgctcc aggccgtcac ttcaaagcac
    19441 gctggtaacg agtcaggttc cactaccagc gaatgatagc gtgtcacggt aagtggattt
    19501 gccagccccc gaaatacgcc ctcaccgtta tgtgtaatcg gcgaggtttt gccgtgcatg
    19561 acctttgcgg cgcgcacaac tttaccgcca aatgcctgcg ccattgcctg atgaccgagg
    19621 cagacgccaa gaatcggcaa gcgcccggca tagtggcgaa taacgtcaag ggagatcccg
    19681 gcttcatctg gcgtacaggg gccaggtgag atgacaattt tttgtggttt aagggcgtcg
    19741 atatccgcca gcgtcaacgc atcgttgcgc ttaaccagca catccgcccc cagttcacaa
    19801 aagtactggt agaggttcca ggtaaaagaa tcgtagttat ctataagcag gatcatggcg
    19861 gctccggtac gaaagaaccg cgctatttta ctcagattcc ccggcttcgc ttaccacttt
    19921 gctaaatatc atctgcaacg cggtgagatc ccccattgcc ccactctgat ttgcctgatt
    19981 ccaggcctct ttttcgatac cctgccagct cagttgatat ccggcatgaa tcgccagttg
    20041 ctcgaagaag atccgctgtg ccagaccact tcccacccgg aagggatgca gcacgttgat
    20101 ttcacaatag tagtgcgcca accgctcgac gaacttcgct ttctccaggc caaccagata
    20161 cccttcctcc tccagatcct gcatcagggc attgccctct ttttcgatat aagcaaagtg
    20221 gcagaacggc gtatcaccct gataaatatc aacttcacgc agttgccctg cccagtcgaa
    20281 aatatcctga tacagctggc gatggatagt tcgcaaatgc ggtaaaccgc gcaccagcgg
    20341 accaagctca atggtcgcag cacgcagcgc cgtcatttcg taagcggcct gttccagccg
    20401 ttgttgctgg cggatgttca gccggttacg catgatatca aggcctggat aaagatacgg
    20461 atcgcgccct tcgccgaatt tatcgctcat agtgactcct cagctcttca aggcgcgcta
    20521 aagcctctgc ggcagtaaga gtgactaaag gcatctcgac gccttcaaga cggcgactgg
    20581 cctgaaaatt acgattacgc tgaagctccc agagacggga cttttgctta tcggtgagtt
    20641 tcttcacttg atgcctccct gaacgtgtcc atttgccaca agtataagcg gcaaatccgg
    20701 gttacgccgg gagagcagaa gcgcgggcaa gattgcccgc gcgaaaaatc attacggcag
    20761 gactttagcg gaaaggataa ctaccggttt tgacggcaca ttctggtacg gaccaacgtc
    20821 atgagtcggc acctgggaaa tcttatcggc aacgtccatg cctttcacca ctttaccaaa
    20881 taccgcgtaa ccgaaatcac gctgaccatg gtcaaggaag gcgttatcgg caacgttgat
    20941 aaagaactgg ctggtggcgc tgtctttgtc agcggtacgc gccatcgcga tggtaccacg
    21001 cgtgttgcgc agcccgttat cggcttcatt tttgattggc gggtttggtt ttttctgctg
    21061 catctgctcg gtgaaaccgc cgccctgaat cataaagcca ggaatgacgc ggtgaaaggt
    21121 agtgttgtta taaaaaccgc tgttcacata atcgacaaag ttttgcacag acactggcgc
    21181 tttttgttta tccagctcca gttcgatgtt accagctgag gttgtcaaca atacgtgcgg
    21241 gtcccctttc gctgccattg ctgcgggaga aagagcagaa agagcgaaaa cagcagccat
    21301 cgccgccagg gtcgatttga acatgagatt tccttaacaa acagagcaga aaaaaagcaa
    21361 gtgaagagat tctaaatagc cttcaagaac aaggccatcc ctttacctaa ttttacgtat
    21421 ctgaaatata tgtaacctga cgatcacttt ttattccgtg atcaaaatca cctcttaaaa
    21481 tgcaatttag caaccgattg caataaaaca tttaaacaga tcacaaaatc acctaaaatc
    21541 gcccgctcgc ggcatatccc gctatgccat tttatttttg aacgcaggcc aatcatgact
    21601 aacagcaatc gcatcaagct cacatggatt agctttctct cctacgcact gactggtgcg
    21661 ttggttattg tcaccgggat ggtgatggga aatatcgccg attatttcaa tctgcctgtt
    21721 tccagtatga gtaacacctt caccttcctc aacgccggca ttttaatctc tatcttcctc
    21781 aacgcctggc tgatggaaat cgtcccgttg aaaacgcagt tacgttttgg ctttctcctg
    21841 atggtgctgg cggttgccgg tttgatgttc agccacagcc tggcgctgtt ctcggcggcg
    21901 atgttcattc tcggggtggt cagcggcatc accatgtcga ttggtacatt cctggtaaca
    21961 caaatgtatg aagggcgtca gcgcggttcc cgcctgttat ttaccgactc cttcttcagt
    22021 atggctggga tgattttccc aatgatcgcc gcgtttctac tggcgcgcag cattgagtgg
    22081 tactgggttt atgcctgcat cgggctggtg tatgtcgcta tttttattct gactttcggc
    22141 tgtgagttcc cggcgctggg caaacatgcg ccaaaaacgg atgctccggt agcgaaagaa
    22201 aagtggggga tcggcgtact gtttctctct gttgcggcgc tgtgctacat cctcggtcag
    22261 ttaggtttta tctcctgggt gcctgagtat gccaaaggcc tgggcatgag cctgaacgac
    22321 gcgggcacgc tggtgagtaa cttctggatg tcatacatgg tcggcatgtg ggcgttcagc
    22381 tttattcttc gcttctttga tttgcaacgc attctgaccg tactggctgg tctggctgcg
    22441 attctgatgt acgtctttaa caccggaaca ccggcacata tggcgtggtc aattctcgcc
    22501 ctgggcttct tctccagcgc gatctatacc accatcatca ccctgggttc acagcagacc
    22561 aaagtaccgt cgccaaaact ggttaacttt gtcctgacct gcgggaccat cggtactatg
    22621 ttgacctttg tggttaccgg cccgattgtt gaacatagcg gtccgcaggc ggcactgctt
    22681 acagcaaacg gtctgtacgc tgtcgtcttt gtgatgtgct tcctgttagg tttcgtcagc
    22741 cgtcaccgtc agcataacac cctgacctct cattaattgc tcatgccgga cggcactatc
    22801 gtcgtccggc cttttcctct caaaccccgc tacgtgcatc tatttctata aacccgttca
    22861 ttttgtctat tttttgcaca aacatgaaat gtcagacaat tccgtgactt aagaaaattt
    22921 atacaaatca gcaatatacc ccttaaggag tatataaagg tgaatttgat ttacatcaat
    22981 aagcggggtt gctgaatcgt taaggtaggc ggtaatagaa aagaaatcga ggcaaaaatg
    23041 agcaaagtca gactcgcaat tatcggtaac ggtatggtcg gccatcgctt tatcgaagat
    23101 cttcttgata aatctgatgc ggccaacttt gatattaccg ttttctgtga agaaccgcgc
    23161 atcgcttatg accgcgtaca cctctcgtct tacttctctc accacaccgc cgaagagctg
    23221 tcgctggtgc gcgaaggctt ctacgagaaa cacggcatca aagttctggt cggcgaacgc
    23281 gctatcacca tcaaccgtca ggagaaggtg attcactcca gcgccgggcg caccgttttt
    23341 tatgacaagc tgatcatggc gaccggttcc tacccgtgga tcccgccaat caaaggttct
    23401 gatactcagg actgctttgt ctatcgcacc attgaagacc tcaacgccat tgaatcctgc
    23461 gcccgtcgca gcaaacgcgg tgccgttgtt ggtggcggcc tgttaggtct ggaagccgct
    23521 ggcgcgctga aaaacttagg tattgaaacc cacgttatcg aatttgcccc tatgctgatg
    23581 gcggaacagc ttgatcagat gggtggcgag cagctgcgtc gcaaaatcga aagtatgggc
    23641 gtgcgcgttc acaccagcaa aaacaccctt gagattgtgc aggaaggtgt cgaagcgcgt
    23701 aaaaccatgc gttttgccga cggcagcgaa ctggaagtcg actttatcgt cttctctacc
    23761 ggtatccgtc cgcgcgataa gctggcaacc cagtgtggtc tggacgttgc tccgcgtggg
    23821 ggtattgtca ttaatgattc ctgccagact tccgatccgg atatctacgc catcggtgaa
    23881 tgcgcaagct ggaacaaccg tgtatttggt ctggtagcac caggctacaa aatggcgcag
    23941 gtcgccgttg accatattct cggtagcgaa aacgcctttg aaggtgctga ccttagcgcc
    24001 aagctgaaac tgctgggcgt agacgtaggc ggtattggtg atgcgcacgg tcgcacacca
    24061 ggcgcacgta gctacgttta cctcgacgaa agcaaagaga tctacaaacg ccttattgtc
    24121 agcgaagaca acaaaaccct gctcggtgcg gtactggtgg gcgataccag cgactacggc
    24181 aacctgctgc aactggtgct gaatgctatc gagctgccgg aaaacccgga ttctctgatt
    24241 ctgccggcgc actcgggtag cggtaagccg tctatcggtg ttgataaact gccggacagc
    24301 gcgcaaatct gctcctgctt cgacgtcacc aaaggtgatc tgattgctgc catcaacaaa
    24361 ggctgccaca ccgttgcggc gctgaaagct gaaaccaaag cgggtactgg ctgcggtggc
    24421 tgtatcccgc tggttactca ggtactgaac gcggaactgg cgaaacaggg catcgaagtt
    24481 aacaacaacc tgtgcgaaca ctttgcttat tcgcgtcagg aactattcca tttgatccgc
    24541 gttgaaggca ttaaaacctt cgaagaactg ctggcgaaac acggcaaagg ttacggttgt
    24601 gaagtttgta aaccgaccgt cggttcgctg ctggcctcct gctggaacga atacattctg
    24661 aagccggaac atactccgct gcaggattct aacgacaact tcctcgctaa catccagaaa
    24721 gacggcacct actcggtgat cccgcgctct ccgggcggtg aaatcacccc ggaagggctg
    24781 atggcggtag gtcgtatcgc gcgtgaattt aatctctaca ccaagatcac tggctcccag
    24841 cgtctggcga tgtttggcgc acagaaagac gatctgccgg agatctggcg tcagctgatt
    24901 gaagccggct tcgaaaccgg tcatgcctat gcgaaagcac tgcgtatggc gaaaacctgc
    24961 gtgggtagca cctggtgccg ctacggcgtt ggcgacagcg tcggcctcgg cgtggaactg
    25021 gaaaaccgct acaaaggcat ccgtacgccg cacaaaatga agttcggtgt ctccggctgt
    25081 acccgtgaat gttcagaagc tcagggtaaa gacgtgggta ttatcgccac tgaaaaaggc
    25141 tggaacctgt atgtttgcgg taacggcggc atgaaaccgc gtcatgcgga tctgctggcg
    25201 gcggatatcg atcgcgaaac gctgatcaaa tatctcgacc gcttcatgat gttctacatc
    25261 cgtactgccg acaaactgac gcgtaccgca ccgtggttag aaaacctcga aggcggcatc
    25321 gattacctga aagcagtgat cattgacgac aaactggggc tgaacgcaca tctggaagaa
    25381 gagatggcgc gcctgcgtga agcggtagtg tgtgagtgga ctgaaacggt caatacgccg
    25441 tctgcgcaga ctcgcttcaa acacttcatc aacagcgaca agcgtgaccc gaacgtacag
    25501 atggtgccag agcgcgaaca gcaccgtccg gcaactccgt atgaacgtat cccagtaact
    25561 ctggtggagg acaacgcatg agccagtgga aagacatctg caaaatcgat gacatcctgc
    25621 ctgaaaccgg cgtctgcgcg ctgttaggtg acgagcaggt cgcgattttc cgcccgtatc
    25681 acagcgatca ggtgtttgcg atcagcaaca tcgacccgtt cttcgagtcc agcgtgctgt
    25741 cacgcggact gattgcggaa caccagggcg agctgtgggt cgccagcccg ctgaaaaaac
    25801 agcgttttcg cttaagcgac ggcttgtgca tggaagacga acagttttcc gtcaaacatt
    25861 acgaagcgcg agtgaaagac ggcgtggtgc agctgcgcgg ttaatgtttt aacgggaggc
    25921 gcaatgcctc ccctttttgc atggtcctgt aataatcttc ggtgtattgc aggacatttt
    25981 ttaaactttt tgttttattt tttgttttta ttttttaaag gataatcaaa tgtttacaga
    26041 cactattaat aagtgtgcgg ctaacgctgc gcgcattgca cgcctgtcgg caaataaccc
    26101 gctcggcttt tgggtcagct ccgccatggc gggcgcgtat gtgggtcttg ggatcatcct
    26161 gattttcacg ctcggtaatt tgctcgatcc atccgtacgc cctttggtga tgggcgcgac
    26221 ctttggtatc gctttaacgc tggtgattat cgccggttct gaactgttca ccggacacac
    26281 catgttcctc acctttgggg tgaaagcggg cgccatcagc cacgggcaaa tgtgggcaat
    26341 cctgccgcaa acctggctgg gtaacctggt cggttccgtc ttcgttgcca tgctctatag
    26401 ctggggtggc ggtagcctgc tgccggtaga taccagcatc gttcactccg tcgcgctggc
    26461 taaaaccact gcaccggcaa tggtactgtt cttcaaaggt gcattgtgta actggctggt
    26521 ttgtctggca atctggatgg cgctgcgcac tgaaggggcg gcgaaattta tcgcaatctg
    26581 gtggtgtctg ctggcattta tcgcgtccgg ctacgagcac tccatcgcta acatgacgct
    26641 gttcgcgctc tcctggttcg gcaaccacag cgaagcctac acgctggcgg gtattggtca
    26701 taacctgctg tgggtgacgc tgggtaatac tttatcgggt gccgtattca tgggattggg
    26761 ttattggtat gctacgccga aagcgaatcg tccggttgcg gacaaattta atcaaactga
    26821 aacggctgcc ggttaattac taaggggttt ttacgtggat catttgccta tattttgcca
    26881 attacgcgat cgcgactgtc tgattgtcgg cggtggtgat gtcgcggaac gcaaagcaag
    26941 gttgctgtta gacgcaggcg ctcgcttaac ggtgaatgca ttagcgttta ttccacagtt
    27001 caccgcatgg gcagatgcag gcatgttaac cctcgtcgaa gggccatttg atgaaagcct
    27061 tctcgacacc tgctggctgg cgattgcagc gacggatgat gacgcgctta accagcgcgt
    27121 cagcgaagcc gctgaagctc gtcgcatctt ctgtaacgtg gtcgatgcgc cgaaagctgc
    27181 cagcttcatt atgccgtcga ttattgaccg ctcaccgctg atggtggcgg tttcctctgg
    27241 cggtacttct ccggttctgg cgcgcctgtt gcgcgaaaaa ctcgaatcac tgctgccgct
    27301 gcatctgggc caggtagcga aatacgccgg gcagttacgc ggtcgggtaa aacaacagtt
    27361 cgccacgatg ggtgaacgcc gccgcttctg ggaaaaactg ttcgttaacg accgtctggc
    27421 gcagtcgctg gcgaacaacg atcagaaagc cattactgaa accaccgaac agttaatcaa
    27481 cgaaccgctc gaccatcgcg gtgaagtggt gctggttggt gcaggtccgg gcgatgccgg
    27541 gctgctgaca ctgaaaggtc tgcaacaaat tcagcaggca gatgtggtgg tctacgaccg
    27601 tctggtttct gacgatatta tgaatctggt acgccgcgat gcggatcgtg ttttcgtcgg
    27661 caaacgcgcg ggataccact gcgtaccgca ggaggagatt aaccagatcc tgctgcggga
    27721 agcgcaaaaa ggcaaacgcg tggtgcggct gaaaggcggc gatccgttta tttttggccg
    27781 tggtggcgaa gagctggaaa cactgtgcaa cgcgggtatt ccgttctcgg tggttccggg
    27841 tattaccgca gcttctggtt gctcagccta ttcgggtatt ccgctcacgc atcgcgatta
    27901 tgcccagagc gtgcgcttaa ttaccggaca cttaaaaacc ggtggcgagc tggactggga
    27961 aaacctggcg gcagaaaaac agacgctggt gttctatatg gggctgaatc aggctgcgac
    28021 tattcagcaa aagctgattg aacacggtat gcctggcgaa atgccggtgg caattgtcga
    28081 aaacggaacg gcagtcacgc agcgcgtgat tgacggtacg ctcacgcaac tcggtgaact
    28141 tgctcagcaa atgaacagtc catcgctaat tattattggt cgggttgttg gcctgcgcga
    28201 taaactgaac tggttctcca accattaatt taacccgggc cagagaattc tgg
//
LOCUS       DAPGOF010000052        28049 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.052, whole genome shotgun sequence.
ACCESSION   DAPGOF010000052 DAPGOF010000000
VERSION     DAPGOF010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28049
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.052"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(116..922)
                     /gene="trpA"
                     /locus_tag="RHV13_001941"
     CDS             complement(116..922)
                     /gene="trpA"
                     /locus_tag="RHV13_001941"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HDV1241476.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRPALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKAFVQPMKAATRS"
     gene            complement(922..2115)
                     /gene="trpB"
                     /locus_tag="RHV13_001942"
     CDS             complement(922..2115)
                     /gene="trpB"
                     /locus_tag="RHV13_001942"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HDV1241477.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(2127..3488)
                     /gene="trpCF"
                     /locus_tag="RHV13_001943"
     CDS             complement(2127..3488)
                     /gene="trpCF"
                     /locus_tag="RHV13_001943"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HDV1241478.1"
                     /translation="MMQTVLAKIVADKAIWVEARKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLILVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(3489..5084)
                     /gene="trpD"
                     /locus_tag="RHV13_001944"
     CDS             complement(3489..5084)
                     /gene="trpD"
                     /locus_tag="RHV13_001944"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HDV1241479.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(5084..6646)
                     /gene="trpE"
                     /locus_tag="RHV13_001945"
     CDS             complement(5084..6646)
                     /gene="trpE"
                     /locus_tag="RHV13_001945"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HDV1241480.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQSLSGNGEALLTLLDNALPAGVENEQLPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNMLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTETAPPLPVVSVPYMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(6668..6761)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(6738..6782)
                     /gene="trpL"
                     /locus_tag="RHV13_001946"
     CDS             complement(6738..6782)
                     /gene="trpL"
                     /locus_tag="RHV13_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HDV1241481.1"
                     /translation="MKAIFVLKAWWRTS"
     gene            6919..7800
                     /gene="yciV"
                     /locus_tag="RHV13_001947"
     CDS             6919..7800
                     /gene="yciV"
                     /locus_tag="RHV13_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HDV1241482.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            7797..8417
                     /locus_tag="RHV13_001948"
     CDS             7797..8417
                     /locus_tag="RHV13_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDV1241483.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            8445..10340
                     /locus_tag="RHV13_001949"
     CDS             8445..10340
                     /locus_tag="RHV13_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HDV1241484.1"
                     /translation="MAGKFRCILLLIAGLFVSSLSYAENTEIPSYEEGISLFDVEATL
                     QPDGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGIPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNNWPF
                     EIYKTRFSLQFSNIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESRDPFYREDFTVL
                     YRWPHALLSNAPAPQTTNIFSHLLLPSTSSLLIWFPCLFLVCGWLYLWKRRPQFTPVD
                     VIETDVIPPDYTPGMLRLDAKLVYDDKGFCADIVNLIVKGKIHLEDQSDKNQQTLIRV
                     NEGVTRNNAVLLPAEQLLLEALFRKGDKVVLTGRRNRVLRRAFLRMQKFYLPRKKSSF
                     YRPDTFLQWGGLAILAVILYGNLSPVGWAGMSLVGDMFIMICWLLPFLFCSLELLFAR
                     DDDKPCVNRVIITLFLPLICSGVAFYSLYINVGDVFFYWYMPAGYFSAVFLTGYLTGM
                     GYIFLPKFTQTGQQRYAHGEAIVNYLARKEAATHSGRRRKGETRKLDYALLGWAVSAN
                     LGREWVARITPSLTAAVSAPEIARSGVLFSLQMHLSLGANTSLLGRSYSGGGAGGGAG
                     GGGGGGW"
     gene            10553..11425
                     /gene="rluB"
                     /locus_tag="RHV13_001950"
     CDS             10553..11425
                     /gene="rluB"
                     /locus_tag="RHV13_001950"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HDV1241485.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(11465..12055)
                     /gene="cobO"
                     /locus_tag="RHV13_001951"
     CDS             complement(11465..12055)
                     /gene="cobO"
                     /locus_tag="RHV13_001951"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HDV1241486.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADPSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(12052..12810)
                     /locus_tag="RHV13_001952"
     CDS             complement(12052..12810)
                     /locus_tag="RHV13_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HDV1241487.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCRSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVIQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            13030..14079
                     /gene="sohB"
                     /locus_tag="RHV13_001953"
     CDS             13030..14079
                     /gene="sohB"
                     /locus_tag="RHV13_001953"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HDV1241488.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(14115..14366)
                     /locus_tag="RHV13_001954"
     CDS             complement(14115..14366)
                     /locus_tag="RHV13_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HDV1241489.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            14746..17343
                     /gene="topA"
                     /locus_tag="RHV13_001955"
     CDS             14746..17343
                     /gene="topA"
                     /locus_tag="RHV13_001955"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDV1241490.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            17553..18527
                     /gene="cysB"
                     /locus_tag="RHV13_001956"
     CDS             17553..18527
                     /gene="cysB"
                     /locus_tag="RHV13_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HDV1241491.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            18821..18985
                     /locus_tag="RHV13_001957"
     CDS             18821..18985
                     /locus_tag="RHV13_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HDV1241492.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            18988..19155
                     /locus_tag="RHV13_001958"
     CDS             18988..19155
                     /locus_tag="RHV13_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1241493.1"
                     /translation="MVGQEQLESSPLCQDSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            19269..19364
                     /locus_tag="RHV13_001959"
     CDS             19269..19364
                     /locus_tag="RHV13_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1241494.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            19528..22203
                     /gene="acnA"
                     /locus_tag="RHV13_001960"
     CDS             19528..22203
                     /gene="acnA"
                     /locus_tag="RHV13_001960"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HDV1241495.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHELAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VVRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIVDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(22267..22857)
                     /gene="ribA"
                     /locus_tag="RHV13_001961"
     CDS             complement(22267..22857)
                     /gene="ribA"
                     /locus_tag="RHV13_001961"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HDV1241496.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            23027..23791
                     /gene="pgpB"
                     /locus_tag="RHV13_001962"
     CDS             23027..23791
                     /gene="pgpB"
                     /locus_tag="RHV13_001962"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HDV1241497.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLTQRICGPLTPPAEENREIAQREQES"
     gene            23940..24248
                     /gene="lapA"
                     /locus_tag="RHV13_001963"
     CDS             23940..24248
                     /gene="lapA"
                     /locus_tag="RHV13_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HDV1241498.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVAPHSSVAKE
                     "
     gene            24255..25424
                     /gene="lapB"
                     /locus_tag="RHV13_001964"
     CDS             24255..25424
                     /gene="lapB"
                     /locus_tag="RHV13_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HDV1241499.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLNNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            25617..26354
                     /gene="pyrF"
                     /locus_tag="RHV13_001965"
     CDS             25617..26354
                     /gene="pyrF"
                     /locus_tag="RHV13_001965"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDV1241500.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDAALAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPYGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            26354..26680
                     /gene="yciH"
                     /locus_tag="RHV13_001966"
     CDS             26354..26680
                     /gene="yciH"
                     /locus_tag="RHV13_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HDV1241501.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(26806..27024)
                     /gene="osmB"
                     /locus_tag="RHV13_001967"
     CDS             complement(26806..27024)
                     /gene="osmB"
                     /locus_tag="RHV13_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HDV1241502.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(27293..28042)
                     /locus_tag="RHV13_001968"
     CDS             complement(27293..28042)
                     /locus_tag="RHV13_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDV1241503.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
BASE COUNT         6871 a         6847 c         7396 g         6935 t
ORIGIN      
        1 acgttcgaca ggggtaagcg aaacggtaaa aagataaata taaaatgaat ttaggatttt
       61 tccggcttcg ttaaagaaag ttaaaatgcc gccagcggaa ctggcggctg ggggattaac
      121 tgcgcgtcgc cgctttcatc ggttgtacaa aagctttcag tgccgccagc attttctctg
      181 gctcattaat atgttgctcg atgattttaa caatggccga accagaaatc gcgcccgcag
      241 ctcctgcatc aatcgctgct tttacctgat ccggggcgga aataccaaat ccctgcaatg
      301 gaggtgcagc gttgtactct ttcagcttcg caaccagatg attgaggggt aacgcggggc
      361 ggttttctgc gccggtcacg cccgctcgtg acagcaaata ggtgtaacca cgaccgtaag
      421 aggctatctg gcgcagcagg tcgtcatcgg catttggcgg gcagatgaag ataggtgcga
      481 cattatgacg caacgcggcc tggcggaagg gcgcggactc ttcaactggc acatcggcaa
      541 ccagcaccga atcgacgccg actttttcgc actgggcata aaactcatca atgcctttgt
      601 taaacaccag attggcatac atcaacaggc caatgggaat ggtcgggtgt ttctggcgaa
      661 tcagtgccag catttcaaaa cattgtgccg gagtcacacc tgccgcaaag gcgcgcagag
      721 tggcgttttg aatggtcggg ccatccgcca gtgggtcgga gaaggggata cctaactcca
      781 gcgcgtcagc accggcttca attagcgtat cgataatttt caatgactgc tcaatgcccg
      841 gatccccgag cgtgacgaaa ggaacgaatg cgccttcttt gcgctccttc aactgggcaa
      901 acagagattc gtagcgttcc atcagatttc ccctcgtgct ttcaaaatat cgtgaacggt
      961 gaagatgtct ttatcgccgc gaccggaaag gttaaccacc agtagctgct ctttttccgg
     1021 gttttcgcgc atcattttca gtgcatgggc cagcgcgtgg gaggattcca gcgccgggat
     1081 aatcccttcg tgcaggcaga gcgttttgaa ggcttccagt gcttcatcat cggtaattga
     1141 cacgtaatca gcgcgtccag tgctgttaag atacgcgtgt tgcgggccga cggacgggaa
     1201 atccagcccg gcagaaatgg agtaagattc ttcaatctgc ccatcttcgg tttgcatcat
     1261 cggcgctttc ataccgaaat agatgcccac gcgaccatgt tttaacggtg cgccgtgctc
     1321 gccggtttct ataccatgac cgccaggctc cacgccaatc aggccgacat tggtttcgtt
     1381 gatgaaatca gcaaacatgc cgatggcatt cgaaccgccg ccaacacagg cgataacggc
     1441 atccggcagg cgaccttctc tttccagaat ctgcgctttg gtttcttcgc caatcatccg
     1501 ctgaaactca cggacaatgg tcggataagg atgtgggccg gctgcggtgc ccagcatgta
     1561 gtgcgcggtt tcgtaactgc cggaccagtc gcgtagcgcc tcattacagg catctttcag
     1621 cgtcgcggaa ccgctatgca ccgggatcac ttccgcaccc attaaacgca tacgaaaaac
     1681 gttaggcgac tggcgctcaa cgtctttggc ccccatataa atacggcatt tcaggccgag
     1741 cagggcgctg gcaagggcag aggccacgcc atgctgaccg gcaccggttt cggcgatgat
     1801 ttcggtttta cccatccgct tcgccagcaa agcctgaccg agtacctggt tggttttatg
     1861 cgcgccgccg tgcagtaaat cttcacgctt caggtacagc gtggtgttcg tgccagccgt
     1921 tatgttctgg catttggtca gtgcggttgg acgcccggca tagtttttca gtaggtcgtt
     1981 gaactgggct tgaaattcag gatctttttg cgcgctgaca aaagcttctt ccagctggcg
     2041 cagagcaggc atcaggattt gtggcacgta catgccgcca aactcaccaa aataggggtt
     2101 aagtaatgtt gtcattgttc ctttccttaa tatgcgcgca gcgtctggaa aaccgaggcc
     2161 aaaagacgtg cgtctttgat gcccggttgc gactctacag cagaattaaa atcaagtccg
     2221 gcgcagccgg tttgtgccgc ttccacgcag ttatctgcgc ctaagccccc cgccagcaga
     2281 acgttgccaa gcgattgacc atttaatagt gaccagtcga aacgttgccc gcttccaccc
     2341 tggccgttgt ctaaaacata tttatcaacg tgctgaagct cgcgggcggg cagggtttca
     2401 ccgacgctta atgctttcca gatggcaacg tgtgctggca gagcttcacg cagcgtatcg
     2461 atatacagct gatcttcatt accatgcagt tgcactgccg ccagcgataa caccttagct
     2521 ttgtccacca catcggcaat atcgtgattg cggaacacgc caacatactg caacggtgcc
     2581 gcagccatca cttcctgcgc ctgttcaacg ttgacgcaac gcggtgatgt cgcaaccaaa
     2641 atcaacccac cgtaaatcgc gcccgcgtca taagctgctt tagcatcttg cccacgcgtc
     2701 aggccacaca ctttattctc acccagcaac acccgacgca cggcggcgtg caaatcgtca
     2761 tgggccatca acgccgaacc aatcagaaaa ccgttagcga agtggcttaa ctcgcgcacc
     2821 tgagcgtaag tattgatgcc tgattcgctg attaccgtca cgttgtgccc cagtttcggc
     2881 gcaagctcgc gggtacggtt gagatcaatc gacaaatcac gcagatcgcg gttgttgatg
     2941 ccaacgacct ttgcccccaa tgcaatggcg cgctccagct cctcttcatt actgacttcg
     3001 gtcagcaccc ccatctccag actgtgagcg acggctgcaa gctggcgata ttggtcgtca
     3061 tccagtactg aaagcattaa taagcaggca tcggcctggt aatagcgcgc cagatagatc
     3121 tggtaagggt cgataatgaa gtctttacat aaaatcggct gcggggcgat ttggctgacg
     3181 atggggagga aatcaaagct cccctgaaaa tatttctcat cagtcagcac cgaaattgcc
     3241 gaagcgtaat gtttataaat ggcggcaatg cgtgctggat cgaaatcatc acggatcacg
     3301 ccttttgacg gcgacgcttt cttgcactcc agaataaacg ccgtgcgcgc accctgtagc
     3361 gcatcataaa aatgtcgcgt gctcggctga acctcattct gaaaactggc cagcggttgc
     3421 tgctgtttgc gggcttctac ccaaatcgcc ttgtctgcga cgattttcgc taaaacggtt
     3481 tgcatcattt accctcgtgc cgccagtgcg gtgactctgt cgtaagcgga accactgcgc
     3541 agtacctcaa gaacggtttg cgcattggct tgcagatctt catggccatg caggcgcatt
     3601 aacatggcga cgttcgcagc gacggctgct tcatgggcgg cgtcgccttt accttgtaac
     3661 aaacgtgtta aaatgtcacg gttttcttcc ggtgttccgc ctgccagttg ctcctggtgg
     3721 tagggcgtca ggccaaaatc ttcagcggtc aattgatagc tcttaatttc gccgtcatgc
     3781 agctcggcaa cgattgtcgg cgcgtgtaat gaaacttcat ccatcccgcc gctgtgcacc
     3841 accgccgcgc gttgataccc cagcacgcgc aaggtttcgg caatcggcag caccagttcc
     3901 ggactataaa caccaattaa cgccagcggc ggatgcgccg ggttaatcaa tggccccagc
     3961 acattgaaca gggtgcgggt tttcagttgc tggcgaaccg gcatcgcgtg acggaatccg
     4021 gtgtgatact tcggcgcaaa gaggaaacaa acacctaact catccagcgc ctggcgcgat
     4081 ttatcggcgt tcatatcaag attaataccg aacgccgcca gcagatcgga cgaaccagat
     4141 ttactggaga cgctacggtt gccgtgtttc gccactttca gcccacaggc cgcggcgaca
     4201 aacgcactgg cggtagaaat attgatactg ttgctgccgt caccgccagt accgacgata
     4261 tcagcaaaca gataatccgg gcgcgggaac ggcgctgcgt tttccagtag cgcggttgct
     4321 gccccggcga tctcgttcgg gtgctcgccg cgaattttca tgctcaccag cgccgccgcc
     4381 agttgttccg gcttcagctc gccacgcacc accgctgaaa acagctggtg gctttcttgt
     4441 tggctaagcg tctgcgcctg atacagtttt tccagaatcg gttgcagcgt gttggctggc
     4501 tctagcttct gctgcgccca ggccagcgtt tgttccagca ggcgagcgcc ctgggtggtg
     4561 agaatggatt ccggatggaa ctggaatcca caaacgcgat ccgcatcgtg acgtactgcc
     4621 atcaccatgc cattaaaatg ggcgttgatg gttaaacctg ccggaatgtt actgccaacc
     4681 agcgagtgat aacgcgccac cggcaatggg tttgttaatc cggcaaacat cgcctgaccg
     4741 tcgtgttcaa tgctggaggc tttaccgtgg agaatttcgc ccgcctgacc gacatagccc
     4801 ccgtaagctt cgacaatcgc ctgatgaccg aggcaaatgc caataatcgg cagcttaccg
     4861 cgcaggcggg taaggagttc tggcatacaa ccggcttcac tcggcacacc tggccccgga
     4921 gaaagcatca gcaccggatt gctcatcgtc gccaggcgtt caattaaggt ttgcgccgga
     4981 atatggttgc ggtaaatcac cacgttatgc ccattgctgc gcaactgatc tgccaggtta
     5041 taagtaaaag agtcgatatt atcgagcagc agaatgtcag ccatcagaaa gtctcctgtg
     5101 catgatgcgc ggtggcgata gcgcgcagta cagcgcgagc tttattacgg gtttcgtcgg
     5161 cttccgactg cggaacagaa tcaaggacta cgccagcacc ggcttgcacg gtggcgatac
     5221 cgttttccac cagcgccgag cggatcacaa tgcaggtgtc gagatcgcca tgcgcggtga
     5281 aataacctac cgcgccgccg tagctgccgc ggcgacgacc ttcagcctcg gcaattaact
     5341 gcatagcgcg tactttcggc gcaccgctta acgtacccat attcatacag gcgcggtaag
     5401 cgtgcagggc gtcaagatcg tggcgcagct caccgaccac gcgagagacc aggtgcatca
     5461 cgtaagagta acggtcaact ttggtaagat cggcgacgta gcggctgccg ggggtgcaaa
     5521 tgcgtgccag gtcattacgg gcgagatcca ccaacatcag atgttcagaa agctctttat
     5581 gatcggtacg catttccagt tcgatgcggc tgtcgaggtc tctgtccagt gaaccatcgg
     5641 cacgacgacc gcgcgggcgt gttccggcaa tcgggtagat ctcaatctgg cggctggtgg
     5701 cgtcatactt gagcgaactt tccggcgacg cgccaaacag ggtgaaatca ttatcctgca
     5761 taaaaaacat gtacgggctg ggattactct ttttcagcac gtaataggcc gccagcggtg
     5821 acgggcaggg cagagagaaa cggcgagatg gcaccacctg gaaaatttct ccggcgcgaa
     5881 tcgctttttg caacaaacgc actacgccac cgaactcttc atcgctctgg ttacattcac
     5941 aacgcatata cggcacggaa accaccggca gcggtggcgc ggtttcggtc agttgctggc
     6001 gaagatcgtt caggcgagca gtgagacgct gtttttcttc ttcattcgga gcaaacagac
     6061 tggcctgaat gcgggtgctt tttttctgat ggtcaatcac catcagcgtt tcagcgagat
     6121 aaaaacagaa atcagggcag ttattttccg ctgacagttg cggtaaatct tcaaaccccg
     6181 ccacaaggtc ataagagaac aggccgccga agaacattgc ttctcgttct tccttcggta
     6241 cattcaacat attctgcaat aagcggaaag cgtcaaaaac cgaaagggag cataagcgag
     6301 cgtcttcatc cagcagtgga ctgacagggg ggaagcgcag cacacggcag tttggtaatt
     6361 gttcattttc cacacccgca ggcagggcgt tatccagtag tgtcagcagg gcttcgccgt
     6421 tgccggaaag ggactggatt gtgacagtgt cacctaatgc tgtaatgcgc agcgcactgt
     6481 ctaccagtag caggcttttt aaatcatctt tgctgtcgat atctgcggat tccagcagca
     6541 gcgttgccgg acgatcccca cacaactggt gaaaaagcgc ggtgggattg tcgcgataag
     6601 cgccttcgca ggttagcagt tcgagagtcg gtttttgtgt ttgcattgtt attctctaat
     6661 tttgttcaaa aaaaagcccg ctcattaggc gggctgggta tctgattgct ttacgcatgg
     6721 tgaatacact gcccgtttca ggaagtgcgc caccaagctt tcagtacgaa aattgctttc
     6781 attgtcgata ccctttttac gtgaacttgc gtactagtta actagttcga tgattaattg
     6841 tcaacagctc atttcagaat atttgccaga accgttatga tgtcggcgca aaaacattat
     6901 ccagaacggg agtgcgcctt gagcgacacg aattacgcag tgatttacga cctgcacagc
     6961 cataccacag cttccgatgg ctgcctgacg ccagaagcat tggtgcaccg tgcagtcgag
     7021 atgcgcgtcg gcaccctggc gatcaccgac catgacacca cagctgcaat cgcgccagca
     7081 agagaggaaa tttcacgttc gggactggcg ctgaatctta ttcccggcgt ggaaatttcc
     7141 acggtctggg aaaatcatga gattcatatt gtcgggctga atattgatat tactcatccg
     7201 ctaatgtgtg agttcctcgc gcagcagaca gaacggcgca atcagcgggc gcagctcatt
     7261 gccgaaagac ttgaaaaagc gcaaatacct ggggcgctgg aaggcgcgca acgactggcg
     7321 cagggcggcg cggtgacgcg cggtcatttt gcgcgtttcc ttgttgagtg cggcaaagcc
     7381 agttcaatgg cggatgtctt taaaaagtat ctggcgcgcg ggaaaaccgg atatgttccg
     7441 ccacagtggt gtacaataga acaagctatt gatgtcattc atcattctgg cggtaaggcg
     7501 gtgctagctc atcctgggcg gtacaatctt agtgctaaat ggctgaaaag attggttgcg
     7561 cattttgccg aacaccacgg cgacgcgatg gaagtcgcgc agtgccagca atcgcccaat
     7621 gaacgcaccc agctggcgac acttgcgcgt cagcatcatt tatgggcatc acaaggatct
     7681 gattttcatc agccatgccc gtggatcgaa ctgggtcgta aactctggct gcccgcaggc
     7741 gttgaaggcg tctggcagct atgggaacag ccgcagaaca ccacagagag ggaattatga
     7801 gccagttttt ttatattcat cctgataacc cacagcaacg tctgatcaac caggcggtgg
     7861 agatcgtgcg taaaggcggg gtgattgttt atccaactga ttccggctat gcgctcggct
     7921 gtaaaattga agataaaaac gccatggagc gtatttgtcg tattcgccag ctgccggacg
     7981 gtcacaactt taccctgatg tgtcgcgatc tttctgaact ctcgacctat tcatttgtcg
     8041 ataacgttgc gtttcgttta atgaaaaaca acacgccggg caactatacc ttcatcctga
     8101 aggggacgaa ggaagtgcca cgccgtctgt tgcaggaaaa acgcaaaacc atcggtatgc
     8161 gtgtgccttc taacccgatt gcgcaggcgt tgcttgaagc actgggcgaa ccgatgcttt
     8221 ccacttcgct aatgctgcca ggcagcgaat ttaccgaatc cgatcccgaa gaaattaaag
     8281 accgtctgga aaaacaggta gatttgatta ttcacggcgg ctatctcggg cagaaaccga
     8341 caacggttat tgatcttacc gatgatacgc cggtcgtggt gcgtgaaggc gtaggtgatg
     8401 tgaagccttt cttataacaa attgcgttcc acggatggaa gactatggca gggaagtttc
     8461 gctgcatttt gctgttgata gcagggctgt ttgtatcatc tctaagttat gcagaaaaca
     8521 cggagatccc ttcttatgaa gaagggatct cgctctttga tgttgaagcc actctgcaac
     8581 cggatggggt gctcgacatc aaagagaaca ttcattttca ggcgcgaaat cagcagatta
     8641 agcacggatt ttatcgtgat ttaccacgac tctggatgca gcctgatggg gacgctgcac
     8701 tgctgaacta tcatattgtt ggcgtcaccc gtgatggtat tcctgaaccc tggcatcttg
     8761 actggcatat cgggttaatg agtattgtcg tgggcgataa acaacgtttc ttgcctcaag
     8821 gcgactatca ttatcaaatt cattatcagg ttaaaaatgc tttcctgcgt gagggggatt
     8881 ctgatctgct aatttggaac gtgaccggta acaactggcc gtttgaaatt tataagaccc
     8941 gtttttctct ccagttctct aatattgcgg gtaatccatt tagcgaaatc gatctcttta
     9001 ccggagaaga gggcgacaca tatcgtaatg gccgcatcct tgaggacgga agaattgaat
     9061 cccgcgatcc gttttatcgt gaagatttca cggtcctcta ccgctggcct cacgctttac
     9121 ttagcaatgc cccggctccg caaacgacga atattttcag ccatcttctt ttaccctcca
     9181 cgtcatcgtt gttaatttgg tttccgtgtc tgttcctggt ttgtggatgg ttatatctct
     9241 ggaagcgcag gccgcaattt acgccggtag atgtgattga aaccgatgtc attccgccag
     9301 attacacgcc cggcatgtta cgtctcgatg cgaagctggt ttacgacgat aaaggttttt
     9361 gtgccgatat cgtaaatctg atagtgaaag gaaaaattca tctggaagat cagtctgaca
     9421 agaaccagca aaccctgatt cgtgttaatg aaggcgtgac cagaaataat gcggtattac
     9481 tgcccgcaga acagttatta ctggaagcgt tatttcgtaa aggcgataag gtcgttctta
     9541 cggggagacg caacagagtc ttacgcaggg catttttacg gatgcagaaa ttttatctgc
     9601 cgcgtaaaaa gtcttcgttt taccggcctg atacgttttt gcaatggggt ggactggcaa
     9661 tattggcggt cattctctac ggtaacctga gtcctgtagg ctgggcagga atgagtctgg
     9721 tgggcgatat gtttattatg atctgctggc ttcttccttt tttattttgt tcccttgagc
     9781 ttttgtttgc ccgcgatgat gacaagcctt gcgttaatcg tgtcatcatc actttgtttt
     9841 taccgctgat ttgttcaggc gtggcctttt attctctcta tatcaatgtc ggagatgtat
     9901 tcttttactg gtatatgcca gcgggttatt ttagcgctgt tttcctgacc ggttatctca
     9961 ctggtatggg gtatattttt ctgccaaagt ttacccaaac tgggcagcaa cgttatgccc
    10021 acggtgaagc tatcgttaac tatcttgcgc gtaaagaggc ggcaacacac agtgggcgtc
    10081 ggcggaaagg ggaaacacgg aaactggatt acgcgttgct aggttgggca gtctcagcaa
    10141 accttggaag agaatgggta gcacgtatca ccccatcact cacagcggct gttagcgccc
    10201 cggaaattgc ccgtagtggc gttttgttct cattacagat gcacctgagt ctgggggcca
    10261 ataccagttt gttggggcga agttattccg gtggtggtgc tggcggcggc gcgggtggcg
    10321 gaggcggtgg tggctggtaa ttaagctgat gttaatcgaa acaagcttta accataaaga
    10381 tagtgtagta tgttgcgcct caaagccagg ccagttaaaa cgtcgagtcg tttacttaag
    10441 gcctgaagag ttcaaacaga ctttactatc agtgagtcag atgtaagttc tgactcactg
    10501 ttgcacaaaa gcatatcaga cgcctgggaa ggcgacacca aaaggaagct ctatgagcga
    10561 aaagctacag aaagtgctgg cgcgtgccgg ccacggttct cgccgtgaaa tcgaatctat
    10621 tattgaagcc ggtcgtgtga gtgttgatgg caaaattgct aaactcggcg atcgcgttga
    10681 agttacccct ggattgaaaa tccgtatcga tggtcacctg atttcggtac gtgagtccgc
    10741 tgaacaaatt tgtcgcgttc tggcctatta caaaccggaa ggtgagttgt gtacccgtaa
    10801 cgatccggaa ggacgtccaa cggtgtttga ccgtctgcca aaactgcgtg gcgcacgctg
    10861 gattgccgtg ggtcgcttgg acgttaatac ctgtggtctg ctgctgttca ccaccgatgg
    10921 tgaactggca aaccgtttaa tgcacccaag ccgtgaagtt gaacgtgaat atgccgtgcg
    10981 tgtatttggt caggttgacg acgcgaaact gcgtgatttg agtcgtggcg tgcagttgga
    11041 agatggtccg gcagctttta aaaccatcaa gttcagcggc ggcgaaggga tcaaccagtg
    11101 gtacaacgtg actctgaccg aaggccgtaa ccgtgaagtt cgtcgtctgt gggaagcggt
    11161 tggtgtgcag gtgagccgcc tgatccgtgt tcgttacggt gatatcccac tgccaaaagg
    11221 tctgccacgc ggtggctgga cggagctgga cctcgcccag actaactatc tgcgcgaact
    11281 ggtggagcta ccgccagaaa ccagctctaa agtcgctgta gaaaaagacc gtcgtcgcat
    11341 gaaggcgaat cagattcgtc gagcggtgaa acgtcacagt caggtgagcg gcggtcgtcg
    11401 ttctggcggg cgtaataacg gttaatcaaa gaatgccgga cgtcatatcc ggcattttta
    11461 cagattaata atcgatccct atctgcgctt ttacaccggc atcaaacgca tgtttgatgg
    11521 ggcgcagctc actcaccgta tccgccagtt caagaatatc ccgatgacag cctcgacccg
    11581 tgataatcac cgtctgttga tgtggacgtt catttaacgc ctgcaccact tcttccagtg
    11641 gcaaatagtc atacgccacc atataagtca gctcatcaag caaaaccata tccagtgaag
    11701 gatcggcaag catccgcttt gcatgttgcc agacttcacg gcaggcagcg gtatcagact
    11761 cgcgattttg tgtatcccag gtaaagcccg ttgccatcac ctgaaactca acgccatgtg
    11821 gctccagcag attgcgttcg ccattaggcc aggtgccttt aataaactgc acgacgccca
    11881 ctttttttcc gtgaccaact gcgcgtgttg ccgtaccaaa tgccgcggtg gttttgcctt
    11941 ttccattgcc ggtaaagacg atgataatac cgcgttcatc ctgggcctgg gccacacgag
    12001 catctacttt ttctttcact cgctgctgac gctgttggta gcgttcatca ctcattggga
    12061 aattcctggt ttacggcccg gttgggcgtc aaaggtcatg ccggttttac ggcggctgtc
    12121 atcgcccatc agccagaggt agagcggcat gatatcagcg ggtgttttaa gtttctgtgg
    12181 atcttcggtc gggaaggcgc tggcacgcat tgcggtgcgc gtaccgcctg ggttaatgca
    12241 gttgacgcgc aggcgctgct gatattcatc ggccagtacc tgcatcatcc cttcggtggc
    12301 aaatttcgac gctgcatatg cgccccagtt ggctcgtccc tgacgtccaa cgcttgatga
    12361 agtaaagacc agtgaaccgg cgtccgattt gagtaataaa ggaagcagtg cctgggtgag
    12421 cataaaggtg gcattaacgt tgacctgtat tacgtcctgc cagacctgtg gattttgttc
    12481 gctcattgga caaacatcgc cgagcaatcc ggcattatgc aaaacaccat ccagacgcgg
    12541 ataattaacg gcaatgcgct gtgccagttg ttggcaatct tcggacctgc aggtcagcaa
    12601 atcgagaata aaccactgtg gctgacgccc agtttcttcg tttatgtggc tggctacctg
    12661 acgtaatttt tcttcattac ggcccaacag aatcactgtc gcaccatagc gtgcatacgt
    12721 catcgcggct tcacgaccaa taccatcact ggctcccgtc accaggataa tgcgatcatt
    12781 gagtaaatct tgttttggct ggtaatgcat ggctactcct caacgacgtt gtctgtcgcg
    12841 ctgccagtgt actttatgac tttggggctt tatgcctgaa acagaagggc atttcaatca
    12901 gcctggggcg ataacaccgc aaaatggata ctttgtcata ctttcgctgc aataacatct
    12961 ctgcgagacg gcttaacatg cctgttgtaa actgtgagcc aaagcgttgt ttaaccaagg
    13021 tggggactcg tggaattgtt gtctgaatat ggtttgtttt tggcgaaaat cgttaccgtt
    13081 gtgctagcga ttgcggcgat tgccgccatt attgtcaatg ttgctcaacg taataaacgc
    13141 cagcgtggcg agttacgggt caacaatctc agcgaacagt ataaggagat gaaagaagaa
    13201 ctggccgcgg cgctgatgga ctcacatcag caaaaacagt ggcacaaagc gcagaagaaa
    13261 aagcataagc aagaagcgaa agcagcaaaa gcgaaagcca aactgggaga ggtggcaact
    13321 gacagtaaac cccgcgtctg ggtgctggat tttaaaggca gcatggacgc ccatgaagtg
    13381 aactcgctac gtgaagagat aacggctgta ctagcagcat tcaaaccgca ggatcaggtt
    13441 gtactacgtc tggaaagccc tggtggcatg gtgcatggtt acgggttggc ggcttcgcag
    13501 ctgcagcgtc tgcgtgataa aaacattcct ttaactgtta cggtagacaa agtcgctgcc
    13561 agcggcggtt acatgatggc ctgtgtggcg gacaaaattg tttccgcacc gtttgctatt
    13621 gtgggttcca ttggggtggt ggcgcaaatg cccaacttta accgcttcct gaaaagcaaa
    13681 gatattgata tcgaacttca caccgccggg cagtataagc gtacgctgac cttgctgggt
    13741 gaaaataccg aagaagggcg ggagaaattc cgcgaagagc tgaacgaaac gcatcagtta
    13801 tttaaagatt ttgtgaagcg tatgcgtccg tctctggata ttgaacaggt ggcaacgggt
    13861 gaacactggt acggacaaca ggcggtagag aaaggcctgg ttgatgaaat caacaccagt
    13921 gatgaagtta ttcttagcct gatggaaggc cgagaagtgg tcaatgtacg ctatatgcag
    13981 cgtaaacgac tcattgaccg attcaccggc agcgcggcag agagcgccga tcgattattg
    14041 ctacgctggt ggcagcgtgg gcaaaagcca ttgatgtaaa agacaaacgc gagggtaaga
    14101 cctcgcgttt tgctttaatc aaccagatga tatttttctg aaagcacatg ggccaggtgt
    14161 ttgaacatat taaacaccgc ggtgcttttg gctgttggca atccttgttc atctaaaaag
    14221 tagtcgccgg taaataccag cacgccatta cgctgtgtga cgccggtggc ttcaatccct
    14281 gcgagcgtat cctcatgctc acgaatgatt ttgttggcct ctttcaacag cgtttcgcgg
    14341 tcgatgggtt gtgtctcttt gttcattatt tactccttaa acaaggacat tagtctacgc
    14401 caggcatggc ttgcagacaa atataccacg ctggtggcaa gagcgcctta ctggcaactt
    14461 tggattttgc atgctaataa agttgcgtat cggattttat caggtacagt gtgacgcttt
    14521 cgtcaatctg gcaatagatt tgcttgacat tcgaccaaaa ttccgtcgtg ctatagcgcc
    14581 tgtaggccaa gacctgttaa ctcagtcacc tgaattttcg tgaacagagt cacgacaagg
    14641 ggttgatatc cgcagagagc gagtccatat cggtaactcg ttgccagtgg aaggtttatc
    14701 aacgtgcgac gcattcctgg aagaatcaaa ttaggtaagg tgaatatggg taaagctctt
    14761 gtcatcgttg agtccccggc aaaagccaaa acgatcaaca agtatctggg tagtgactac
    14821 gtggtgaaat ccagcgtcgg tcacatccgc gatttgccga ccagtggctc agctgccaaa
    14881 aagagtgccg actctacctc caccaagacg gctaaaaagc ctaaaaagga tgaacgtggc
    14941 gctctcgtca accgtatggg ggttgacccg tggcacaatt gggaggcgca ctatgaagtg
    15001 ttgcctggta aagagaaggt cgtctctgaa ctgaaacaac tggctgaaaa agccgaccac
    15061 atctatctcg caaccgacct tgaccgcgaa ggggaagcca ttgcatggca cctgcgggaa
    15121 gtgattggtg gtgatgatgc gcgctatagc cgagtggtgt ttaacgaaat tactaaaaac
    15181 gcgatccgcc aggcatttaa caaaccgggt gagctgaata ttgatcgtgt taatgcccag
    15241 caggcgcgtc gctttatgga ccgcgtggtg gggtatatgg tttcgccgct gctatggaaa
    15301 aagatcgctc gtggtctgtc tgccggtcgt gtgcagtcgg tggcagtccg cctggtggtc
    15361 gagcgtgagc gtgaaattaa agcgttcgtg ccggaagagt tctgggaagt cgatgccagc
    15421 acgaccacgc catctggtga agcgttggcg ttgcaggtga ctcatcagaa cgacaaaccg
    15481 ttccgtccgg tcaacaaaga acaaactcag gctgcggtaa gtctgctgga aaaagcgcgc
    15541 tacagcgtgc tggaacgtga agacaaaccg acaaccagta aacctggcgc tccttttatt
    15601 acctctacgc tgcaacaagc tgccagcacc cgtcttggat ttggcgtgaa aaaaaccatg
    15661 atgatggcgc agcgtttgta tgaagcaggc tatatcactt acatgcgtac cgactctact
    15721 aacctgagtc aggacgcggt aaatatggtt cgcggttata tcagcgataa ttttggtaag
    15781 aaatatctgc cggaaagtcc gaatcagtac gccagcaaag aaaactcaca ggaagcgcac
    15841 gaagcgattc gtccttctga cgtcaatgtg atggcggaat cgttgaagga tatggaagca
    15901 gatgcgcaga aactgtacca gttaatctgg cgtcagttcg ttgcttgcca gatgacccca
    15961 gcgaaatatg actccacgac gctgaccgtt ggtgcgggcg atttccgcct gaaagcacgc
    16021 ggtcgtattt tgcgctttga tggctggaca aaagtgatgc ctgcactgcg taaaggcgat
    16081 gaagatcgta tcttaccggc agtcgataaa ggcgatgctc tgacgctcgt tgaactgaca
    16141 ccagcccagc actttaccaa accgccagcc cgtttcagtg aagcatcgct ggttaaagag
    16201 cttgaaaaac gcggtatcgg tcgtccgtct acctatgcgt cgatcatttc gaccattcag
    16261 gaccgtggct atgtgcgtgt agaaaaccgt cgtttctatg cggaaaaaat gggcgaaatc
    16321 gtcaccgatc gcctggaaga gaatttccgc gagttaatga actacgactt caccgcgcag
    16381 atggaaaaca gcctcgacca agtggcaaat cacgaagcag agtggaaagc tgtactggat
    16441 cacttcttct cggatttcac tcagcagtta gataaagctg aaaaagatcc ggaagagggg
    16501 ggtatgcgtc cgaaccagat ggttctgacc agcatcgact gcccgacctg tggtcgcaaa
    16561 atggggattc gcacagcgag caccggggta ttccttggct gttctggcta tgcgctgccg
    16621 ccgaaagagc gttgcaaaac caccattaac ctggtgccgg aaaacgaagt gctgaacgtg
    16681 ctggaaggcg aagacgctga aaccaacgcg ctgcgcgcaa aacgtcgttg cccgaaatgc
    16741 ggcacggcga tggacagcta tctcatcgat ccgaaacgta agttgcatgt ctgtggtaat
    16801 aacccaacct gcgacggtta cgagatcgaa gagggcgaat tccgcattaa aggttatgac
    16861 ggcccgatcg ttgagtgtga aaaatgtggt tctgaaatgc acctgaaaat ggggcgtttc
    16921 ggtaaatata tggcctgcac caacgaagag tgtaaaaaca cgcgtaagat tttacgtaac
    16981 ggcgaagtgg ctccaccgaa agaagatccg gtaccattac cggagctgcc gtgcgaaaaa
    17041 tcagatgcct atttcgtgct gcgtgacggt gctgccggtg tgttcctggc ggccaatacc
    17101 ttcccgaaat cgcgtgaaac gcgtgcgccg ctggtggaag agctgtatcg cttccgcgat
    17161 cgtctgccgg aaaaactgcg ttatctggcc gatgcgccgc agcaggatcc ggaaggtaat
    17221 aagaccatgg ttcgctttag ccgtaaaacc aaacagcaat atgtctcttc ggaaaaagac
    17281 ggaaaggcga ctggctggtc agcattttat gttgatggca aatgggttga aggaaaaaaa
    17341 taacctttaa ttctgtcagg tttttataaa caaagggtcg cgaaagcggc ccttttttat
    17401 tgcatattat tttttcttca cacctataca ctaaggctat aaatgatata gtggttatag
    17461 ttagcacctt ttttattatt aaatcgtatt agtcacccgc ctggtgtgac gaaaaaacga
    17521 tgttctgatg gcgtctaagt ggatggttta acatgaaatt acaacaactt cgctatattg
    17581 ttgaggtggt caatcataac ctgaatgtct catcaacagc ggaaggactt tacacatcac
    17641 aacccgggat cagtaaacaa gtcagaatgc tggaagacga gctaggcatt caaatttttt
    17701 cccgaagcgg caagcacctg acgcaggtaa cgccagcagg gcaagaaata attcgtatcg
    17761 ctcgcgaagt cctgtcgaaa gtcgatgcta taaaatcggt tgccggagag cacacctggc
    17821 cggataaagg ctcactgtat atcgccacca cgcataccca ggcacgctac gcattaccaa
    17881 acgtcatcaa aggctttatt gagcgttatc ctcgcgtttc tttgcatatg caccagggct
    17941 cgccgacaca aattgctgat gccgtctcta aaggcaatgc tgatttcgct atcgccacag
    18001 aagcgctgca tctgtatgaa gatttagtga tgttaccgtg ctaccactgg aatcgggcta
    18061 ttgtagtcac tccggatcac ccgctggcag gcaaaaaagc cattaccatt gaagaactgg
    18121 cgcaatatcc gttggtgaca tataccttcg gctttaccgg acgttcagaa ctggatactg
    18181 cctttaatcg cgcagggtta acgccgcgta tcgttttcac ggcaacggat gctgacgtca
    18241 ttaagactta cgtccggtta gggctggggg taggggtcat tgccagcatg gcggtggatc
    18301 cggtcgccga tcccgacctt gtgcgcgttg atgctcacga tatcttcagc cacagtacaa
    18361 ccaaaattgg ttttcgccgt agtacttttt tgcgcagtta tatgtatgat ttcattcagc
    18421 gttttgcacc acatttaacg cgtgatgtcg ttgatgcggc tgtcgcattg cgctccaatg
    18481 aagaaattga ggtcatgttt aaagatataa aactgccgga aaaataattt cttgcgttat
    18541 tttcggcacc ttttttgtag cgaaggtgcc gaaatatatt ttcttttgtt acttatttgt
    18601 atatcgtgca caatatttta tccctcatat ttatagggta aattacctgt aaagtagtgt
    18661 ctaattaccc gcctcgcaaa ctccagtttt caaatatcta tttcaggtca aaagattgaa
    18721 ttattcactg aagacgattc gtaaattcgc tggatttttg actaaagttt ctttaggatt
    18781 tgtctcatcg tatgattaat tacaccaatc gttcaacgtc ttgctgataa gtgatggcga
    18841 ttatatgagg ttagcaatgc cttctggaaa tcaggaaccc cgccgcgatc ctgaattaaa
    18901 acgtaaagcc tggctggcgg tttttcttgg ttctgcactt ttctgggtgg ttgtcgcact
    18961 gctgatttgg aaagtgtggg gataactatg gttggtcagg agcaactgga gtcgtcacca
    19021 ttatgccagg atagtgacaa tgaaccagaa acgaaacggg aatgttccgt cgttattcca
    19081 gacgactggc aactaacatc gcagcagcaa gcctttatag aactgtttgc tgaagatgat
    19141 cagccgaaac aataattatc atcattctta ttacccattt ttaatgaatt aaagggcttt
    19201 taatacaccg cagcaataac aggttgagtt atctcaacac aaagtaataa ccgttaaggg
    19261 tgtagcctat gatcaacaca aatatgaaat attggtcctg gatgggcgcg ttttctctgt
    19321 cgatgctctt ctgggccgaa ctcctctgga tcattactca ctgatccttg accccgctgc
    19381 ggcggggttt tcatttgctt tgccacaagc tttctcctct tttatcaatt tgggttgtta
    19441 tcaaatcgtt acgcgatgtt tgtgttatct ttaatattca ccctgaagag aatcagggct
    19501 tcgcaaccct gtcattaagg aggagctatg tcgtcaaccc tacgagaagc cagtaaggac
    19561 acgttgcagg ccaaagataa aacttaccac tactacagcc tgccgcttgc tgccaaatca
    19621 ctgggcgata tcacccgtct acccaagtca ctcaaagttt tgctcgaaaa cctgctgcgc
    19681 tggcaggatg gtaactcggt taccgaagag gatatccacg agctggcggg atggctgaaa
    19741 aatgcccatg ctgaccgtga aattgcctac cgcccggcaa gggtgctgat gcaggacttt
    19801 accggcgtac ctgccgttgt tgatctcgct gcaatgcgcg aagcggttaa acgcctcggc
    19861 ggcgatactg caaaggttaa tccgctctca ccggtcgacc tggtcattga ccactcggtg
    19921 accgtcgatc gttttggtga tgatgaggca tttgaagaaa acgtacgcct ggaaatggag
    19981 cgcaaccacg aacgttatgt gttcctgaaa tggggaaagc aagcgttcag tcggtttagc
    20041 gtcgtgccgc caggcacagg catttgccat caggttaacc tcgaatatct cggcaaagca
    20101 gtgtggagcg aattgcagga cggtgaatgg attgcttatc cggatacact cgttggtact
    20161 gactcgcaca ccaccatgat caacggcctt ggcgtgctgg ggtggggcgt tggtgggatc
    20221 gaagcagaag ccgcaatgtt aggccagccg gtttccatgc ttatcccgga cgtagtgggc
    20281 ttcaaactta ccggaaaatt acgtgaaggt attaccgcca cagacctggt tctcactgtt
    20341 acccaaatgc tgcgcaaaca tggcgtggtc gggaaattcg tcgaatttta tggtgatggt
    20401 ctggattcac taccgttggc ggatcgcgcc accattgcca atatgtcgcc agaatatggt
    20461 gccacctgtg gcttcttccc aatcgatgct gtaaccctcg attacatgcg tttaagcggg
    20521 cgcagcgaag atcaggtcga gttggtcgaa aaatatgcca aagcgcaggg catgtggcgt
    20581 aacccgggcg atgaaccgat ttttaccagt acgttagaac tggatatgaa tgacgttgaa
    20641 gcgagcctgg cagggccaaa acgcccacag gatcgcgttg cactgcccga tgtaccaaaa
    20701 gcatttgccg ccagtaacga actggaagtg aatgccacgc ataaagatcg ccagccggtc
    20761 gattatgtta tgaacggaca tcagtatcag ttacctgatg gcgctgtggt cattgctgcg
    20821 ataacctcgt gcaccaacac ctctaaccca agtgtgctga tggccgcagg cttgctggcg
    20881 aaaaaagccg taactctggg cctcaagcgg caaccgtggg tcaaagcgtc gctggcacca
    20941 ggctcgaaag ttgtttctga ctatctggca aaagcgaaac tgacaccgta tctcgacgaa
    21001 ctggggttta accttgtggg atacggttgt accacctgta ttggtaactc tgggccgctg
    21061 cccgatccta tcgaaacggc aatcaaaaaa ggcgatttaa ccgtcggtgc agtgctgtcc
    21121 ggcaaccgta actttgaagg ccgtatccat ccgctggtta aaaccaactg gctggcctcg
    21181 ccgccgctgg tggttgccta tgcgctggcg ggaaatatga atatcaacct ggcttctgag
    21241 cctattggtc atgatcgtaa aggcgatccg gtttatctga aagatatctg gccatcggca
    21301 caagaaattg cccgtgcggt agaacaagtc tccacagaaa tgttccgcaa agagtacgca
    21361 gaagtctttg aaggcactgc agagtggaaa gaaattaacg tcgtacgatc cgatacctac
    21421 ggatggcagg aggattcaac ctatattcgt ttatcgcctt tctttgatga aatgcaggca
    21481 acaccagcac ctgtggaaga tattcacggt gcgcggatcc tcgcaatgct gggggattca
    21541 gtcaccactg accatatctc tccggcgggc agtattaagc ccgacagccc ggcaggtcga
    21601 tatctacaag gtcggggtgt tgagcggaaa gactttaact cctacggttc gcggcgtggt
    21661 aaccatgaag tgatgatgcg cggcaccttc gccaatattc gcatccgtaa tgaaatggtg
    21721 cctggcgttg aaggggggat gacgcggcat ttacctgaca gcgacgtagt ctctatttat
    21781 gatgctgcga tgcgctataa gcaggagcaa acgccactgg cggtgattgc cgggaaagag
    21841 tatggatcag gctccagccg tgactgggcg gcaaaaggtc cgcgtctgct tggaattcgt
    21901 gtggtgattg ccgaatcgtt tgaacgaatt caccgttcga atttaattgg catgggcatc
    21961 ctgccgctgg aatttccgca aggcgtgacg cgtaaaacgt tagggctaac cggggaagag
    22021 aagattgata ttgtcgatct gcaaaatcta caacccggcg cgacggttcc ggtgacgctt
    22081 acgcgcgcgg atggtagcca ggaagtcgta ccctgccgtt gtcgtatcga caccgcgacg
    22141 gagttgacct actaccagaa cgacggcatt ttgcattatg tcattcgtaa tatgttgaag
    22201 taacaagtat ttgcttgccg gttattttgc ttccggcaag caaaagaatt acacaatgca
    22261 agagggttat ttgttcagca aatgacccat tttctcggct ttggtatcga gataatgttc
    22321 gttattgggg ttacgaccta caatcaacgg tacgcgttca acaatattaa ttcctgcttc
    22381 ggtcagaatt tcgacttttt tcgggttatt ggttaacaag cggacttcat tgacgccaag
    22441 aagtttgaac atatcagcgc aaagagtgaa gtcgcgctca tcagcggcga agcctaactg
    22501 gtggttagcc tctacagtat cgtaaccttg gtcctgcagt gcgtaagcgc ggattttatt
    22561 cagcagacca atgttacgac cttcctgacg gtgatacagc aaaataccac ggccttcctc
    22621 ggcaatttgc gtcaatgccg cttcgagctg gaagccacaa tcgcagcgca agctgaacag
    22681 ggcgtcaccg gtcagacatt cggaatggac gcgcgcaagt accggggtat gcccggaaat
    22741 atcgccatag actagcgcga catgatcgtg tccggttgcc agttcttcaa atcccaccat
    22801 caggaaatcg ccccatgggg ttggcagttt ggcttctgcc acacgtttaa gctgcatgaa
    22861 attctccaga taatgctggt tctgtattgg cttattttgc cataacgaga aggggttcac
    22921 ctaatcacga cgtgtcgatc gttcacggaa tggcacaaat ctgtcaattt ttcctggaac
    22981 tggcattttc agttatgatt gtgggactta tcaaaaagga gaggccatgc gttcgattgc
    23041 ccgacgtacc gcagtgggag ctgcactatt gcttgttatg ccagtagccg tatggatttc
    23101 tggctggcgt tggcaacctg gagaacaaag ttggctacta aaagcggctt tttgggttac
    23161 tgaaactgtc acccagccct ggggcgtcat tacacatttg attttattcg gctggtttct
    23221 ctggtgtctg cgttttcgca ttaaggctgc ctttgtatta tttgccattc tggcggcggc
    23281 aatccttgtg ggacaaggcg ttaaatcctg gatcaaagac aaagtccagg aaccaagacc
    23341 ttttgttatc tggctggaaa aaacacatca tattccggtt gatgagttct acactttaaa
    23401 gcgagcagaa cgcggaaatc tagtgaaaga acagttggct gaagagaaaa atattccaca
    23461 atatttgcgt tcacactggc agaaagagac ggggtttgcc tttccttccg gtcacacgat
    23521 gtttgctgcc agttgggcac tgctggccgt tggtttgctg tggccgcgtc ggcgaacgtt
    23581 aaccattgct atcttgttgg tctgggcaac gggagtcatg ggaagccgcc tgctgctcgg
    23641 gatgcattgg ccacgcgatc tggtggtagc tacgttgatt tcgtgggcgc tggtggcggt
    23701 ggcaacgtgg cttacgcaac gaatttgtgg gccattaaca ccgcctgcgg aagaaaatcg
    23761 cgaaatagcg caacgagaac aagaaagtta acgctggttg attttccgaa tttagccctt
    23821 aaatcatcaa caatgcgcgt ggatgccatt tcgcagacgg cgcgaagatg gtactttaaa
    23881 gggctattgc ggtaagttga ccataattta ttcgctctaa ccacataacg ggaagtaatg
    23941 tgaaatattt actcattttc ttactggtgt tagcgatctt cgtgatttcg gtcacgttgg
    24001 gtgcgcagaa cgatcaacag gtgacgttta attatctgtt agcgcaaggg gagtaccgta
    24061 tttccacatt gctggcggta ttgtttgctg cgggttttgc tatcggttgg ttgatttgtg
    24121 gcctgttctg gctgcgagtt cgtgtttccc tggcgcgcgc tgaacgtaaa ataaagcgac
    24181 tggaaaacca gctttcaccc gcgactgacg tggctgtagc gccgcactcg tcagtggcga
    24241 aggaataact ttctatgctg gagttgttgt ttctgctttt gcctgtagcc gctgcctatg
    24301 gctggtatat gggccgcaga agtgcgcaac aaaacaagca agatgaagcc aaccgcttgt
    24361 cgcgtgatta cgtagcgggg gttaacttcc tgcttaataa tcaacaggat aaagcggtag
    24421 acctgtttct cgatatgctt aaagaggata caggcaccgt tgaagcccac cttacgctcg
    24481 gaaacctgtt ccgttcgcgt ggcgaagttg atcgcgctat tcgcatccat cagaccctaa
    24541 tggaaagcgc ctcgctgacc tatgaacagc gtctgttggc gattcaacaa ctggggcgtg
    24601 attacatggc cgctgggtta tacgaccgcg cggaagacat gttcaatcag ctgaccgatg
    24661 aaactgactt ccgcattggt gcgctgcaac agttgctaca aatctaccag gctaccagcg
    24721 agtggcagaa agcaattgat gttgccgaac gcctggtgaa gctgggtaaa gataaacagc
    24781 gcgtcgaaat tgcccatttc tactgtgagt tagccctgca gcatatggcc agcgacgatc
    24841 tcgatcgtgc catgaccttg ctaaaaaaag gggcggcggc agataaaaac agcgcccgcg
    24901 tatccatcat gatgggacgc gtgtttatgg cgaaaggaga atacgccaaa gccgtcgaaa
    24961 gtttgcaacg cgtgatatcc caggacagag aactggtcag cgaaacgctg gaaatgttgc
    25021 aaacctgcta tcagcagttg ggtaaaactg ccgaatgggc agaattcctg cagcgcgcgg
    25081 tggaagagaa caccggcgcc gatgctgaat tgatgcttgc ggatatcatc gaagcgcgcg
    25141 acggtagtga ggctgcacag gtctatatta cgcgccagct tcagcgtcat ccgaccatgc
    25201 gtgtgttcca taagttaatg gattaccact taaatgaagc ggaagaaggg cgtgccaaag
    25261 agagcctgat ggtgctgcgt gacatggttg gcgaaaaggt acgtagtaag cctcgttatc
    25321 gctgccagaa atgtggtttt accgcataca ccctctactg gcattgtccg tcttgtcggg
    25381 cctggtcaac cattaaaccg attcgcggtc ttgatggcct gtaatttttt aaaaaaaccg
    25441 actttagtta caacatacta gttattgatg tcccattgtg ctccggcaat gacggcgcag
    25501 aaaaagcctg ccaggggaga aatcgcaact gttaattttt tatttccacg ggtagaatgc
    25561 tcgccgttta cctgtttcgc gccacttccg gtgcccatca tcaagaaggt ctggtcatga
    25621 cgttaactgc ttcatcttct tcccgcgctg ttacgaattc tcctgtggtt gttgcccttg
    25681 attatcataa tcgtgatgcc gcgctggcct ttgtcgacaa gatcgaccca cgcgattgtc
    25741 gtctgaaggt cggcaaagag atgtttacat tgtttgggcc acagtttgtg cgcgaacttc
    25801 aacagcgtgg ttttgatatc tttcttgacc tgaaattcca cgatatcccc aacactgcag
    25861 cgcacgctgt cgctgctgca gctgacttag gcgtgtggat ggtgaatgtt catgcctctg
    25921 gtggggcgcg tatgatgacc gcagcgcgtg aggcactggt tccgtatggc aaagatgcac
    25981 cgcttttgat tgctgtgaca gtgttgacca gcatggaagc cagcgacctg gccgatcttg
    26041 gcgtgacact gtcacctgca gattatgcag aacgtctggc ggcactgacg caaaaatgtg
    26101 gccttgatgg tgtggtgtgt tctgctcagg aagctgtgcg ctttaaacag gtattcggtc
    26161 aggagttcaa actggttaca ccgggcattc gtccgcaggg gagtgaagca ggtgaccagc
    26221 gccgcattat gacgccagaa caggcgttgg cggctggtgt tgattatatg gtgattggtc
    26281 gcccggtaac gcaatcggta gatccagcgc agacgctgaa agcgatcaac gcctctttac
    26341 agcggagtgc atgatgagtg attccaacag ccgtctggtc tactcaacag aaaccgggcg
    26401 tattgatgag cccaaagcgg ccccagtacg ccctaaaggc gatggtgtgg tgcgtattca
    26461 gcgtcagacc agtggacgta aaggtaaggg cgtttgcctg attaccggtg tcgatctcga
    26521 tgatgccgaa ctgacaaaac ttgcagcgga actgaagaaa aaatgcggct gcggcggagc
    26581 agttaaagat ggagttattg aaatccaggg cgataagcgt gatttattaa aatcactact
    26641 tgaagcgaaa gggatgaagg taaaactcgc aggcggttaa cataaaaagc cacggatata
    26701 tccgtggctt tcgaatattt tactgtgtgt attattcagt tatttcgttc tgaacagaat
    26761 caagcgtatt ataattgcga gtcgataatc gacatttatt tacgattatt taccgacctg
    26821 gtgaccaata acaccaccga cagctgcgcc acctaatgta cccaacgtac tgccatcggt
    26881 cagtactgca ccgcctaatg cccctgcacc cgcgccgatt gcggtgttgc ggtcccgttt
    26941 agaccagtta gaacaggcac tcagagacat tgccagggta attgccagaa cagccgcggt
    27001 catttttttg ctcgttacaa acataatact ctctcctgaa tttatgattc acggaagtaa
    27061 gctctctata actataatag ctaagaataa gcctgatgaa attattccgt gaaatccgct
    27121 cgcgcgcagg gttatatcac gcaggtgata atgacttcct gttatatcgc tgataataat
    27181 tttatatctt gagagtgtta ataacaggta aatagtctta attatcaacc aggaatcatc
    27241 ttagagcgga tgatttgcca aactgcaaat catccgtaga gaagggaaat ggttaaatgt
    27301 caacgacgtg aacggtgagt ttactgtgct caagatgcat cagatcgctg gcgcgtattt
    27361 tcgaatcggt aatcacgcga ttgaatcgct caacgggacc gatggagtat gaatgtacag
    27421 caccaaattt cgagctgtca gtcaggacta tcgcttcgca ctctttttcc agcacggcat
    27481 tcaccacatc ggtacgcatc atatcgcgac cggtgaatcc agtttcaggt tgccagccat
    27541 caataccaat aaatgctttg ctgaaatgta cctgttggat gcactggcgt gtcaacgggc
    27601 caaccatact ttcgcttttt ttctggtaca cgccaccgag caaaataact tcacaaggcg
    27661 cgtctttcag caaatgcgcg atgtagctgc tgaccgtgat gatagtgaca tttttcttct
    27721 gctcccccag agtccgggcg agcagggcat tgctgctgcc attttcgata aagatggttt
    27781 cgcccggttg aaccagtgac gcggcaaact cggcgagttc acgcttcagc gtatagttgc
    27841 tcatcatccg ggtttcgacg tcatcactat caagcgaaac tgcaaaacca tgtgcacggc
    27901 ggaggtaact cagtttttcg agggtgttga gatcctggcg aatggtaact tcagaaactc
    27961 cagtggcttt tgccagatcg gttacgctaa cctgaccctg gtcaatgacc atctgtagaa
    28021 tagtttgttg tcgggaattc atcgctgta
//
LOCUS       DAPGOF010000053        27770 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.053, whole genome shotgun sequence.
ACCESSION   DAPGOF010000053 DAPGOF010000000
VERSION     DAPGOF010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.053"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            19..1374
                     /locus_tag="RHV13_001969"
     CDS             19..1374
                     /locus_tag="RHV13_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HDV1241504.1"
                     /translation="MARFQFKNRKNNGLIFLISFMVMGEAAIAAPLPQWANAPAVTPV
                     AQLSLQESILRAFARNPDVTQQAAQIGIGEAQIDEAKSAWYPHVGLTGNAGPSRQTDS
                     SGRLDNNVSYGITLTQLVYDFGKTNNDINLQTAARDSYRFKLMATLTDVAEKTATAYM
                     EVSRYQVLCDAAQRNIHSLENVYNMAALRANAGLNSSSDELQAQTRIAGMRSTLEQYQ
                     AQMASAKAQLAVLTGVQPEAIAAPPAELAEQPVSLKNIDYQSIPLVLAAENLRQSAQY
                     GVEKTKAQYWPTLSIQGGKTRYQTSDRSYWDDQLQLNVNAPLYQGGAVSAQVQQAEGQ
                     QKISASQVEQAKLDVLQRASVAYANWTGARGREEAGLAQSESAHKTRDVYQNEYKLGK
                     RSLNDLLTVEQDVFQAQSAEINANYDGWVAAVNYAAAVNNLIPLAGIKQGLYNDLPDL
                     K"
     gene            1483..3372
                     /locus_tag="RHV13_001970"
                     /pseudo
     CDS             1483..3372
                     /locus_tag="RHV13_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243339.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            3393..22258
                     /locus_tag="RHV13_001971"
                     /pseudo
     CDS             3393..22258
                     /locus_tag="RHV13_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612540.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            22262..24424
                     /locus_tag="RHV13_001972"
     CDS             22262..24424
                     /locus_tag="RHV13_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HDV1241505.1"
                     /translation="MKKNAQSVEAWLEAMIAVARYYRLDFSQENVRATVNWERDSKRE
                     ELLTDMARQLGMGLRLVEFSADSLNPWRLPLIAVFDNQQIGVITRRDNYDNISVQFSG
                     DEGLETTLNIADIEDKIVELALLRPLSAIPDARVDDYIRPYQANWFWSLSLKDWRRYG
                     DIMLASLVANVLALAAMIFSMQVYDRVVPAQSYPTLWVLFAGVMMAILFEFCMRMVRT
                     HLSDMIGKRADLRISDRVFGHALRLKNNVRSKSTGSFISQIRELESVRELITSTTIGA
                     VADLPFFLLFVFILWMIGGWLVLVVLLALPLLVIPGLLVQRPLARLANEGMRESAVRN
                     ATLVEAVQSIEDIKLLRAEQRFQNQWNHTNDVASSISMKQRFLTGLLLTWTQEVQSIV
                     YVVVLLVGCFMVMNGDMTTGALVGTTILASRTIAPLSQISGVLSRWQQAKVARNGLDE
                     LMKRPVDQPEHGKLVHKAVLHGNYQFSNAVFYYDEEEKIADVAIGKLNIQAGEKIAIL
                     GRNGAGKSTLLQMLAGMRIAQQGQVLLDNISIGQLDPADLRRDMGLLSQTGRLFFGSL
                     RENLTMGMPEASDEDIERALTLSGALPFVQKQKNGLNYMIQEGGFGLSGGQRQTLLLA
                     RLLISQPNIVLLDEPSASLDEMAEAHLIEQLKQWIGHRTLIIATHRTAMLQLVDRIIV
                     MDQGRIVMDGAKEAILREQGEPTARRVVLQEKNKGSAA"
     gene            24421..25596
                     /locus_tag="RHV13_001973"
     CDS             24421..25596
                     /locus_tag="RHV13_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HDV1241506.1"
                     /translation="MNGQLQYNRGLTEPRLPRSALAVRVTAVMLLCFLGWAWYFQLDE
                     VTTGSGTVEPSGREQVVQSLEGGILYHLDVKVGDIVEQGQPLAQLNRTKTESDVQEAM
                     SRLYAALATSARLRAEVSNKPLVFPDELNKFPELIESETALYNTRRDGLNKATTGLTQ
                     GISLVNRELAMTQPLVKQGAASSVEVLRLQRQANELENKLSDVRTQYYVQAREELAKA
                     NAEVETQRSVIRGREDSLTRLNFTAPVRGIVQDIDVTTVGGVIAPGGKLMTIVPLDEQ
                     LLIEAKISPRDVAFIHPGQKSLVKITAYDYSIYGGLPGEVAVISPDTVQDEVRRDVYY
                     YRVYIRTFSNHLENKSKQQFPIFPGMVATVDIRTGKKSVLDYLLKPFNKAQEALRER"
     gene            25593..26000
                     /gene="cueR"
                     /locus_tag="RHV13_001974"
     CDS             25593..26000
                     /gene="cueR"
                     /locus_tag="RHV13_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HDV1241507.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     NQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(26204..26626)
                     /locus_tag="RHV13_001975"
     CDS             complement(26204..26626)
                     /locus_tag="RHV13_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1241508.1"
                     /translation="MMNSSIKSFSLLAVILLAGCSSPASRIADCQAQGVSHDTCYLAE
                     QQRQTAILSASEAQAFKNAEAAQHAQAAKKAIYKGFGMTFRMSSKNFAYLNDSLCAID
                     EDNKDATVYQSGLYNVIVYHHTGKVALMKEGQFVGYLK"
     gene            complement(26712..27728)
                     /locus_tag="RHV13_001976"
     CDS             complement(26712..27728)
                     /locus_tag="RHV13_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA family autotransporter adhesin"
                     /protein_id="HDV1241509.1"
                     /translation="MKTVNVALLALIISATSSPVVLAGDTIEAAATELSAINSGMSQS
                     EIEQKITRFLERTDNSPAAYTYLTEHHYIPSETPDTTQTPTVQTDPDAGQKTVAATGD
                     VQTTARYQSMINARQSAVTDAQQTQITEQQAQIVATQKTLAATGDTQNTAHYQEMINA
                     RLAAQNEANQRTTTEQGQKMNALTTDVAVQQQNERTQYDKQIQSLAQESAQAHEQIDS
                     LSQDVTQTHQQLTNTQKRVADNSQQINTLNNHFSSLKNEVDDNRKEANAGTASAIAIA
                     SQPQVKTGDVMMVSAGAGTFNGESAVSVGTSFNAGTHTVIKAGVSADTQSDFGAGVGV
                     GYSF"
BASE COUNT         6329 a         8080 c         8188 g         5173 t
ORIGIN      
        1 gaaggaagtg acttatctat ggcgcgattc cagtttaaaa accgtaaaaa taatggactt
       61 atttttttaa taagttttat ggtgatggga gaagctgcaa ttgctgcacc gctgccacaa
      121 tgggcgaacg ccccagcggt aacgccagtt gcccaattat ccttacagga aagtatatta
      181 cgcgcctttg cgcgaaatcc cgacgtcact caacaggcgg cgcagatagg cattggcgaa
      241 gcgcaaattg atgaagccaa aagtgcctgg tatccgcacg ttggattaac cggcaacgcg
      301 gggccgtccc gacaaacgga ctccagcggc aggcttgata acaacgtttc gtatggcata
      361 accctgacac aactggtgta tgactttggt aaaactaaca acgatatcaa tctgcaaact
      421 gccgcccgtg acagctaccg ctttaaattg atggctacct taaccgatgt tgcagagaaa
      481 acggcgactg cctatatgga agtcagtcgt tatcaggttt tgtgcgatgc ggctcaacgc
      541 aatattcact cgctggaaaa cgtctacaac atggcggcat tgcgcgctaa cgctggcctg
      601 aactcatcgt cggatgaatt acaggcccag acgcgtattg ctggaatgcg ctcaacgctg
      661 gagcaatatc aggcgcagat ggcgagcgcc aaagcgcaac tggcggtgct cactggcgta
      721 cagccggagg cgatagccgc gccacctgct gaattagccg agcagccggt atcgctgaag
      781 aatattgatt accagtccat cccgctggtg ctggcggcag aaaacttacg ccagtcagca
      841 cagtacggcg tggaaaaaac gaaagcgcaa tactggccaa cgctcagtat tcaggggggt
      901 aaaacgcgct accagaccag cgaccgctcg tattgggatg atcagctaca actgaacgtt
      961 aacgcgccgc tgtatcaggg cggcgcggtt tctgcccagg tgcaacaggc cgaagggcaa
     1021 caaaaaatct ctgcctcgca ggtcgaacag gcaaaactgg atgtgctgca acgagcgtct
     1081 gtggcatatg caaactggac cggcgcacgg ggtcgcgaag aagccggttt agcgcaatcc
     1141 gaaagtgcgc acaaaacgcg agatgtgtac caaaatgaat ataaattggg taaacgcagt
     1201 ctgaatgatc tattaaccgt cgagcaagat gtctttcagg cgcaatcggc tgaaataaat
     1261 gccaattatg atggctgggt cgccgccgta aattatgccg ctgcggtgaa taaccttatt
     1321 ccattagcgg gaattaaaca gggcttatac aacgacttac ccgacttgaa ataatcctgc
     1381 cattattaat atcaggtatg cctcgttgtt aagtgattac ctttgtctta tttagataaa
     1441 tggactcgct aaggatgtgc gccatttatc aggaatgcca ttatgagcct gattattgat
     1501 gttatttcgc gtaaaacatc cgtcaaacaa acgctgatta atcctggcga cgtcacggtt
     1561 gttatttatg agccttccgt ggtgcaggtt catgctcagg cctctgccgt tgcgcgttac
     1621 gtccgtgaag gaaatgacct gctgatctat atgcaggacg gcacggtgat ccgctgcaac
     1681 ggttatttcc tgcaagcggc gaatacagct gaacaatcgg aactggtgtt tgccgatggt
     1741 caacagctaa cccatatcac ctttgccgat actgctgcgg gtggattagc ccccgtagaa
     1801 ctgactgccc agaccactgc gattgaaagc attgcgccat ttcttgatac cgttgctcag
     1861 accagcgcct tcccgtgggg gtggctggcg ggggcggcgg taggtggtgg cgcgcttggt
     1921 gcactgctgg caagcggtgg agatggcgac tcgaaaacag aagtgattaa taaccctacg
     1981 ccacctgctg agcctggcaa cgccacacca tcatttttag ttaccgataa tcagggcgat
     2041 cagcgcggca ttctcgccac caatgacatc accgatgaca ctacgccaac ctttagcggc
     2101 agcgggcagg cgggggcgac tattcagatt aaagacagta acggcaatac tattgccagt
     2161 actcaggtag acaacaacgg tcactggagt gtctcgttac ccacgcaaag tgcaggtgaa
     2221 catacctggt cagtggtgca aattgtcggc agtaccatca ctgacgccgg ttcgataacg
     2281 ttaaccatcg acaatagtca ggccagcgtg caggttgcca ccaccgcagg cgataacatt
     2341 attaacgcca gcgaacaggc cgccgggttt acgctttctg gcaccagtag tcatctggca
     2401 cagggaacag aactcaccgt cacacttaac ggcaaaacct acacgaccag cgtaggcgct
     2461 aacggtgcct ggagcgtgca ggtgccgacc gccgatgcac aggcgttagg cgaagggaat
     2521 caggcggtgc tggtcagtgg gaaagacgcc acaggcaata cggtcaccgg cgcgcagcta
     2581 ctaacggtcg atacccaacc gccaacgctt gccatcaaca ccatcgctca ggacaacatt
     2641 atcagtgctg cggaacataa cgcctcgctg gtactgagcg gcacgtcgaa tgcagaagcg
     2701 gggcaaaccg taacactgac cgtcaacggg aaaagccata cagcacccgt cggtagcgac
     2761 ggaacctggc aagtgacgct gcctgccacg gaagtccagg cactggcgga gggtaattac
     2821 gctgtcaatg ccagtgtcag cgatcgggca gggaacacca ccagccacag cgcgaatttc
     2881 acggtagaca cctcagcacc cgtggtcagt gttaataccg tggcgggcga cgatattctt
     2941 aataatgccg agcaggccgt cgcgcagatc atctccggac aagttagcgg tgcttctcct
     3001 ggcgatacgg taacggtgaa attgggcact catgtcctga cgggcatcgt gctggcagat
     3061 ggcagctgga atgtggcgct ggacccggcg gtaacccgca cgctggatcg cggagccaat
     3121 acgattttcg tcaccgtgac agatgctgca ggaaatactg gcgcggcgtc tcgagcaatc
     3181 acgctggtcg gtgtttctcc gttgatcacc attaacaccg tctccggcga tgacattatc
     3241 agtggcgcag aaaaaggtgc gccactgacc cttaccggta gcactcaaca ggctgagaca
     3301 ggacaaaccg tcacagtaac cctggctgga cagagtttta ccactaccgt gcaggccgat
     3361 ggctcctgga gtctgaggta cctgccgccg cgatgggaaa tctgcctgac ggcgcggtgg
     3421 cgattaccgc ttctgtgacg gatctcagcg gcaataccgg caacacctcc cgcaccatta
     3481 ccgtcgacag ccaggccccg gccttaagca ttgatccact gaccgctgat aacatcatta
     3541 acgccgccga aagcgggcag gatctgccca tcaccggcac caccgacgcc cagccagggc
     3601 agacggtaac ggtgacgctt aatgggcaga cgtatcaggg cgtcgtgcag ccagacggca
     3661 cctggagcgt gaccgttccc gccgccaacg ttgacgcgct ggctgacggt aacgccacgg
     3721 tcaccgccag cgtgaacgat gtcgccggta acccgaccag cgtctcacgc gtggcgctgg
     3781 tcgatgccac gccgccggtg gtaaccatta atccggtggc gaccgataac gtcatcaaca
     3841 cgccggaaca tgctcaggcg caaatcatca gcggcacggt tactggcgct caggcgggcg
     3901 atatcgtcac cgtgacgctg aataatgtgg attacaccac ggtggtggat ggttccggca
     3961 actggagtct gggcgttccg gcctcggtgg tcagtgggct ggcggacggc agttatcctg
     4021 tcagcgtctc ggtaaccgac aaagccggaa acacgggcag ccagtcactg actgtcacgg
     4081 tcaataccgc agcgcccctt atcggcatta acagcattgc gggcgatgat gtgattaatg
     4141 ccagcgaaaa aggggccgat gtccagatta ccggcaccag cgatcagcca gcgggtacag
     4201 ccatcaccgt gacgctgaac gggcaaaatt atacggcgac gaccgacgcc tccggcaact
     4261 ggagcgtcac cgttccggca tcggcggtga cagcattagg ccaggccaac tacacggtaa
     4321 cggcggcggt aaccagcagt atcggcaaca gcgccaccgc cagccataac gtgctggtcg
     4381 acagtgcgct ccccggcgtg accattaacc cggtggcaac cgacgatatt attaacgccg
     4441 ccgaagcggg cgtggcgcaa tccatcagcg ggcaggttac tggagccgcc gttggcgaca
     4501 cggtaacgat tacgctgggc ggcaatactt acaccaccac cgtgcaggcc aatttaagct
     4561 ggagcgtgag cgttccggcg gcagatattc aggcgttggg caatggcgat ttaacggtta
     4621 atgcctcggt caccaatcaa aacggcaata ccggcagcgg cacgcgtgat atcaccatcg
     4681 acgctaatct gcccggcctg cgggtcgata cggtggcggg cgatgatgtg gtcaatatca
     4741 tcgagcatgg gcaggcgctg gtgattaccg gcagcagctc ggggctggct gaaggtacgc
     4801 cgcttaccgt cacgattaat aatgtggaat acaccaccgc ggtgcaggcc gatggtagct
     4861 ggagcgtggg cgtaacggcg gcgcaggtta gcgcctggcc tgcgggggcg gttaatattg
     4921 ccgtatccgg ggaaagtagc gccggaaatc cgataagcat tacgcatccg gtaacggtgg
     4981 atctcactcc ggcagcgatc accatcaaca ccatcgccac ggacgatgtg attaacgccg
     5041 cagaaaaagg cgctgattta accctttccg gcaccaccac taacgtagaa cccggtcaaa
     5101 ccgtcaccgt cacctttggc gggaaaaatt acactgccag cgtagcgagc gatggtagct
     5161 ggactgccac cgtacccgcc gccgatctgg cgtcattacc cgagggcagc gcctccgcac
     5221 tggccagcgt cagcaatatc aacggcaata gcgcctcggc ggtgcacaac tacagcgtcg
     5281 acagcagcgc gccaaccatc attatcaata ccgtcgccag cgacaatatc gtcaacgcca
     5341 gcgaagccga tgcgggcgtg acggtgagcg gcagtaccac cgccgaagcg gggcagattg
     5401 ttacggtaac gctcaatagc ccgaccgtgc agacctatca ggcaacggta caggccgatg
     5461 gcagctggag catcaatatt ccggcggcag atctcgcagc cttgaccgat ggcagccata
     5521 ccctgacggc gacggtcaat gacaaagcgg gcaacccggc gagcaccacg cataatctgg
     5581 cggtggatct caccgttccg gtgctgacca tcaacaccat tgcgggcgat gacattataa
     5641 acgccaccga acacgggcag gcgctggtga tttccggttc cagcactggc ggagaagcgg
     5701 gagatgtcgt caccgtcacg ctaaacagta aaacctacac cacgacgctg gatgcctccg
     5761 gcaactggag cgtcggtgtt ccggcggcgg atgtcaccgc gctgggtagc ggcccgcaaa
     5821 ccgtgactgc gaccgttacc gatgccgccg gtaacagcga tagtgagacg cacacgctca
     5881 ccgttaacct taccgcgccg accattggca tcaatatcat cgccaccgat gacataatta
     5941 acgccagcga aaaaggcgcg gatctgcaaa tttccggtac cagcaatcag ccagcaggca
     6001 ccacaataac ggtgacgctg aacgggcaaa attacactgc caccacagat gcttccggca
     6061 actggagcac tacagtgcca gcttccgctg tcggtgcgct gggcgaagcc agctacacgg
     6121 tgacggcgaa cgtcaccgac agcgcgggca acagcaattc cgccagccat aacgtgcagg
     6181 tgaataccgc gctgcctggc gtcaccctta atccggtagc gagcgacgat attatcaacg
     6241 ctgccgaatc gggcgtggcg cagaccatca gcggccaggt cacgggcgcg gcggcgggcg
     6301 atacggttac cgtaacgctt ggcgggaaaa cctacaccgc taccgtgcag gggaatttaa
     6361 gctggagcgt gaacgttccg gctgcggata ttcaggccat cggtaatggc gacctgacgg
     6421 ttaacgcctc ggtcaccaac ggcgttggca ataccggcag cggttcgcga gatattacta
     6481 tcgacgccaa tttgcctggc ctgcgcgtcg ataccgtggc gggcgatgat gtggtcaata
     6541 gcatcgagca cgctcaggcg ctggtgataa ccggtagcag cagcgggctg gcggcgggcg
     6601 cggcgctgac ggtagtcatt aacacggtca cttatgctgc aacagtatta gccgatggca
     6661 catggagcgt tggcgtgccg gcagcagacg tgggcaactg gcctgcgggt acagtgaata
     6721 tcattgtaag cggcactaac actgcagaaa cgactacgac tatcacctat ccagtcaccg
     6781 tcgatctggc agctgtggca atttccatta acaccgtttc cggcgacgat gtgattaatg
     6841 ccgctgaaaa aagtgcagat ttaacccttt ccggcagcac ctccggcgtg gaagtggggc
     6901 aaacggtcac cgttaccttt ggcgggaaaa cctacaccgc taccgtagcg ggcgatggta
     6961 gctggacaac caccgtgcct gccgccgatc tcagcgtgtt acgcgacggc gacgccacgg
     7021 tacaggccag cgtcagcact attaacggca acacggcttc ggcaacgcac gcctacagcg
     7081 ttgatgccac ggcaccgacg cttgccatta ataccatcgc caccgacgat attctgaacg
     7141 ccgccgaagc gggcaatccg ctaaccatca gcggcagcag taccgccgaa gcggggcaga
     7201 cggtaaccgt cacacttaat ggcgtgactt acagtggcac cgttcaggcg gacggtagct
     7261 ggagcgtcag cgtaccgaca acagatctca gcaatctgac cgccagccag tacacggtca
     7321 gcgcctcggt aagtgataaa gcaggcaacc cagcgtccac cactcacggg ctggcggtgg
     7381 atctcaccgt tcccgtatta accatcaaca ccgtctccgg cgatgacatt attaacgccg
     7441 ccgaacacgg gcaggcgctg gtgatctccg gttccagcac tggcggcgaa gcgggtgatg
     7501 tcatcaccgt cacactaaac agtaaaacct acaccaccac gctggacgct tccggcaact
     7561 ggagcgtcgg cgttccggcg gcagacgtta ctgcccttgg cagcggtccg taaaccatca
     7621 ctgtggcaat taccgatgcg gctggcaaca gcgatgacgc cagccgcacg gtcaccgtaa
     7681 atctcgccgc gccaaccatt ggcatcaaca ccatcgccag cgatgacgtg attaacgcca
     7741 cggaaaaagg tgcggacctg caaattaccg gcaccagtaa tcagcccgca ggcaccacca
     7801 ttacggtgac gctgaacggg caaaattaca ccgccaccac agatgcttcc ggcaactgga
     7861 gcgccacggt gccagcctcg gcagttagcg cattgggtga agctaactac acggtaaccg
     7921 cgagcgtcac cgacacggca ggcaacagta attccgccag ccataatgtg ctggtcaaca
     7981 gcgccttgcc tgccgttacc attaacgcgg tggcgactga cgatattatt aacgcagccg
     8041 aatcgggaaa tgcgcaaacc atcagcgggc aggtgacggg cgcagcgcag ggggatacgg
     8101 ttaccgttac gctgggcggc aacacctaca ccgccacggt gcaggctaat ttaagctgga
     8161 gtgtcagcgt tccggcggcg gatattcagg cgctgggtaa cggcgacctg acggttaatg
     8221 cctcggtcac caatggcgtg ggcaacaccg gcagcggctc gcgcgatatc acgatcgacg
     8281 ccaatctgcc tggcctgcgg gtcgataccg tggcgggcga tgatgttatt aacagcattg
     8341 agcacaatca ggcgctggtg atcaccggca gcagcagcgg attaacggcg ggaacggcgt
     8401 taacggtcga gattaataac gttacttatg gcgcgacggt attagccgac ggcacgtgga
     8461 gcctcggtgt tccggcggta gacgtcagca actggccagc gggtacggtg aatatcacgg
     8521 taagcggcac caacagtgcc ggaacaacct ccaccattac ccatccggtc accgtcgatc
     8581 tggctggggt tgccatcacc attaacacac tctccggcga tgatgtgatc aacgccgttg
     8641 aaaaaggcga aacgctggtc gtaagcggca gcaccagcgg tgtcgaatcc gggcagacgg
     8701 tgaccgtcac ctttggtggc aaaaattaca ccactaccgt ggaagccaac ggcagctgga
     8761 cggtgaatgt gccgcctgcc gatctcgctg cgctgccgga tggcgcgggc aacgtgcagg
     8821 cgagcgtcag caacatcaac ggcaataacg cccaggcgga tcgcgcgtac agcgttgatg
     8881 ccaccgcgcc gcttgtgacc atcaacatca tcgccagcga cgatattctt aatgtgagcg
     8941 aagcgggcgc ggggatcacc atcagcggta ccaccacggc gcaggccggg cagacgctga
     9001 ccgtcacgct caataacaac acgtaccaga ccaccgttca ggccgatggc acctggagcg
     9061 tggatgttcc ggcagcggat ttaagcgggc tgaccgccag cagctatacc gtgaccgcca
     9121 cggtgagcga caaagcggta acccggcaag cgccgaccac gcgctggcgg tagatatcac
     9181 tgcgccggat ctcaccatta acaccgtcgc gggcgatgac attatcaacg ccatcgaaca
     9241 tggtcaggcg ttggtggtca gcggcaccag tacgggcgcg gcggcgggtg atgtggtaac
     9301 cgtcacactg aacggtaaaa actacaccac cacgctggat gcctccggta actggagcgt
     9361 gggcattccg gcggcggatg tcacggcgct ggcgaccggt agccagacca tcaccgccag
     9421 cctgagcgat cgcgcgggca acagcgacag cacgactcac gatgtgaccg ttgatcttag
     9481 cggcccgacg ctgaccatta acaccgtctc cggcgatgac attattaata atgcggagaa
     9541 aacccaggat ctgaccatca gcggcggctc cagcggtctg gcgactggca ccacggtcac
     9601 cgtgatgttg aacggcctgg cgtacagcgc caccaccgat agttccggca actggagcgt
     9661 taccgtgcct gcttcggcgg tcggcgcact gggtgaggcg gtgtatcaga ttagcgccag
     9721 cgccaccgac agcgcgggca acagcggcag caccacccat acggtgaatg tcgaatcgct
     9781 cctgcctggg gtgatcatta ataccgttgc gggcgatgac attatcaacg ccgctgaaat
     9841 cgttgtggcg cagaccatca gcggtcaggt cacgggaacg gcggttgccg ggaatacggt
     9901 gattgtcacc attggcggca atcaatataa cgccaccgtg cagtcagatt taagctggag
     9961 cgtcagcgta ccggcgaacg ttttgcaggc gctgggtaac ggtgaactga ccatcagcgc
    10021 ctcggtgacc aattccgcaa ataataccgg caccgcgacg cacgatatcg tgatagatgc
    10081 caacctgcca ggtctgcgcg tcgataccgt ggcaggcgat gatgtgatta acagcattga
    10141 gcacactcag gcggtggtgg ttaccggcag tagcaccggg ctggcagcgg gcgcggcgtt
    10201 gacggtggtt atcaatggcg tcacctacgg cgcaacggta ctcgccgatg gcagctggag
    10261 cgtgggcgtt tcggcggcgg acgtgggtaa ctggcctgcg ggaacggtca atattgccgt
    10321 ctctggcacc aacaccgccg gaaccacaac cagcattagc catccggtca cggtcgatct
    10381 cgctgccgtg gcgatcacca tcaacaccct ttccactgat gatgtgatta acgccgccga
    10441 aaaaggcagc gatttgcaac tctccggcac cacctccggc gtggaagcgg ggcagactat
    10501 taccgttatt ttcggtggta aaagttacac caccacggtt gccgcggata atacctgggg
    10561 gctgacaatc cctgtagccg atgttgcaac cttgccagac ggcgcagcaa acgtccaggc
    10621 cagtgtcagc aatgtcgcag ggaacagcgc ccaggcaacg catgcttaca gcgttgatgc
    10681 cactgcgccc tccgttacca tcaacaccat cgccacggac gatattctta acgccgccga
    10741 agcgggatcg gcgctgacca tcagcggcac cagcacggcg gaagccgggc agacggtgac
    10801 cgtaacgcta aacggcgtta attacagcgg caatgtgcag gcagacggga gctggagcgt
    10861 cagcgtacca accggcgatt tagctagctt taccgccagc tcgtataccg ttaacgcctc
    10921 ggtcagcgac aaagccggaa atccggcctc ggcgacgcat aacctgacgg tggaccttgc
    10981 cgctccggtc gtcaccatca acacggtggc gggcgatgac atcatcaacg ccacggaaca
    11041 cggacaggcg cagattatca gcggctcggc aacgggcgcg actaccggta atacggtttc
    11101 cgtgacgatt ggcacgacca cctataccac cgtgctggac gccaacggca actggagcat
    11161 cggcgtgcct gccagcgtga tttccgcact ggcgcagggc gatgtaacca ttaccgccac
    11221 ggtcaccgac tccgcaggca acagcggcac ggcctcgcac actgtcaccg tggcgctcgg
    11281 cgctccggtg ctcgccatta acaccattgc cgtcgatgac atcatcaacg ccacggagaa
    11341 aggcgcagat ctggcgattt ccggcaccag cgaccagcct gcgggcacgc agattaccgt
    11401 tacgcttaac gggcaaaatt acaccaccac tgccgatgct tccggtaact ggagcgtgac
    11461 cgttccggcg tcacgggtga gcgccctcgg tgaagccact tacacggtga ccgccgccgc
    11521 tactgactct gatggcaaca gtggttccgc cagccataac gtgcaggtta ataccgcgct
    11581 gccgggcgtc accattaacg tggtggcaac ggacgatatc attaacgctg ccgaagcggg
    11641 cgcgggccag agcatcagcg ggcaggtgac gggcgcggca gcgggcgaca cggtgaccgt
    11701 cacgctcggc ggggcgactt acactgccac ggtgcaggcg aatttaagct ggagcgtcga
    11761 tgttccggcc tccgcgctac aggcgttggg caacggtgag ctgaccattt cggcttcggt
    11821 aacgaacagc gtaggcaata ccggcaacgg cacgcgtgaa atcaccatcg acgcgaatct
    11881 gccaggtctg cgggtcgata ccgtggcggg cgatgacgtg gtcaacatta tcgagcacgg
    11941 gcaggcgctg gtgattaccg gcagcagctc cgatctggca gcgggcagca acgtcacgct
    12001 gaccataaac gggcaaacct atgttgcggc ggtgctggcg gatggctcat ggagcgtcgg
    12061 tgttccggcg gcagatgtca gcgcctggcc tgcggggacg gtcacgatta cagccagcgg
    12121 caacaccacc gccggaaacc cggtgagcgt tacgcatccg gtgactgtcg atctctcggc
    12181 ggtggcggtc agcatcaacg ccattaccgc cgatgatgtg attaacgccg ccgaaaaagg
    12241 cgcggcgtta acgctctccg gcagcacctc tggcgtcgaa gccgggcaaa cggttaccgt
    12301 cacctttggc ggcaaaactt acagcgccac ggtggctgct aacggttcct ggagcacctc
    12361 ggttccggcg gcagatatgg cggccctgcg cgatggcgat gccagcgcgc aggccagcgt
    12421 cagcaatgtt aacggcaaca gcgccaccac tacccacgct tacagcgtcg atgccaccgc
    12481 gccaacggtg accattaaca ccattgcggg cgacgatatt cttaacgctg ccgaagccgg
    12541 cgcggcgctg accatcactg gcagcagcac ggcggaagcg gggcagacgg tgaccgtcac
    12601 cctcaatggc acaaactata ccggcaccgt acagacggac ggcagctgga gcgtcagcgt
    12661 gccgccagcc gacttaagcg ccctgaccgc cagcaactac accgtgaacg cggcggtgag
    12721 cgacaaagcc ggaaacccgg cctcggttaa tcacaacctg acggtggata cttctgttcc
    12781 ggtcgtcacc atcaacacgg tggcaggcga tgatgtgatc aacgcgacgg aacacgccca
    12841 ggcgcagatc atcagcggct ccgccaccgg tgcggcaacc ggtagcaccg tgacagtgac
    12901 tatcggcaca aataccttta ccacggtgct ggatgccagc ggcaactgga gcatcggcgt
    12961 tccggcaagc gtcgtctcgg cactggcgaa cggcacggtg accattaacg ccagcgtcac
    13021 tgatgccgga ggaaacagcg gcagcaccac ccatcaggtg acggtcaata ccgggctgcc
    13081 gaccattacc tttaacgcta tcagcggcga taacgtcctg aacgccgatg aaaaaggcca
    13141 gccgttgacc attagcggta gcagtacggg gctggcgaca ggcgcacagg tcaccgtcac
    13201 gctcaacggt cataactaca gcgctaccac cgacgcggcg ggcaactgga ccttaaccgt
    13261 tccggtcagc gatctggcgg ctctaggcca ggccaactat acggtcagcg ccagcgccac
    13321 cagtgcggca ggcaacaccg ccagcagcca ggcgaattta ctggtcgaca gcggcctgcc
    13381 aggcgtcacc attaacaccg tagcgggcga tgatattatc aacgccgccg aagcgggtgc
    13441 cgatcaaacc atcagcgggg tggtgacccg cgccgccgct ggcgatacgg tcaccgtgac
    13501 gctgggcggc aacacttaca cagcggaggt tcagcccgat tttagctgga gcgtcagcgt
    13561 tccggcagcc gatctccagg cgctgggcaa tggcgatttg accattaccg cctcagtcac
    13621 caacgctaat ggcaacactg gcagcggtac gcgggatatc accattgatg ccaacctgcc
    13681 tgggctgcgg gtggataccg tcgcgggcga tgatatcgtc aacagtatcg agcacgggca
    13741 ggcgctggtg atcaccggcg gcagtagcgg cctgaatgca ggtgctgtgc tgacggttac
    13801 catcaacagt gtggcgtatt ccgccacggt acaggcggac gggagctgga gcgtcggtat
    13861 tccggcggca aacgtcagcg cctggccagc gggggcatta accgttgagg tagacgggca
    13921 aagcagcgcc aataacccag tcagcgtcag ccatccgttc accgtcgatt taacggcggt
    13981 ggcaatcagc atcaacaccg ttgccagcga cgacgtgatt aacgccgcag aaaaaggcac
    14041 caatctgact ctttccggca gtaccagcgg gattgagagc gggcaaaccg tcaccgtcac
    14101 ttttggcggt aaaacctaca ctgcaagcgt cgccgccaac gggagctgga gtgtaaacgt
    14161 tccggcggca gatctggcaa ctctgccaga cggcgcggcg aacgtgcagg ccagcgtcag
    14221 cagcgcgagc ggcaacagtg cctcggtgac tcatgcgtat agcgttgacg ccagcgcgcc
    14281 gacgctcacc attaacacca tcgccagcga cgatatcctt aacgccacag aggcgggaaa
    14341 tccgctcacc atcagcggca ccagcaccgc cgaaaccggg cagacggtga ccgtcaccct
    14401 taacggcgca acctacaccg gcaatgtgca ggcggacggt agctggagcg tcagcgttcc
    14461 cacttcagcc ttaggcgcgc tgaccgcaag caattacacc gtcagcgcca cggtcaacga
    14521 caaagcgggc aaccccggca gcgccagcca caatctggcg gtagatacca ccgcgccggt
    14581 tctcaccatt aacaccgtgg cgggcgatga catcatcaac gacgccgaac acgcgcaggc
    14641 gctggtgatc tccggcacca gcacgggcgg ggaagcgggc gatgtggtga gcgttgtgct
    14701 caacggcaaa acctacacca ccacgctgga tgcctccggt aactggagcg ttggcgttcc
    14761 ggcggcagat gttacggcgc tgggtagcgg tgcgcagacc atcaccgcca gcgtcagcga
    14821 tcgggcaggc aacagcgatg acgccagccg caccgtcacc gtcagcctca gcgcgccagt
    14881 gattagcatc aacaccatcg cgggcgatga tgtgatcaac gcggcggaaa aaggatctga
    14941 tctggcgctt tccggcacca gcgatcagcc agcgggcacg gcgatcaccg tcacgctgaa
    15001 cggacaaaac tacagcgcca ctaccgacgc ctccggcaac tggagtgtca ccgtgcctgc
    15061 ctcggcggtc agtgcgctgg gcgaagccac ctacagcgtg acggcgagcg tcaccaacgc
    15121 tcagggtaac agcagcaccg ccagccataa cgtgcaggtc aataccgcgc tgccgggcgt
    15181 caccattaat ccggtggcga cggacgatat tatcaacgct tcagaggcag gcagcgcgca
    15241 aactatcagc ggccaggtaa ccggtgcggc ggcggacagc accgtcaccg ttgaactggg
    15301 cggtaaaact tacaccgcca ccgtgcagcc agatttaagc tggagtgtga gcgtgcctgc
    15361 cgccgactgg caggcgctgg gtaacggtga gttaacggtt aacgcctcgg tgaccaacgc
    15421 cgttggcaac accggtagcg gcacgcgcga tatcaccatc gatgccagtc tgcctggcct
    15481 gcgggtggat accgtcgcgg gtgatgatgt ggtcaatatc atcgaacaca gtcaggcgca
    15541 ggtgatcacc ggtagcagct ccggctttgc cgcaggtaca gcgctgacgg tggtgataaa
    15601 taatcaaact tacgccgcga cggtgctggc gaatggtacc tggagcgtcg gcgttccggc
    15661 ggcggatgtc agcaactggc ctgcgggaac gctgaatatt accgttagcg gggcgaacag
    15721 tgccggaacg caaaccagca ttacccatcc gctgaccgtt gatctcacta ccgtcgccgt
    15781 cagcatgaac agcatcacca gcgatgatgt gattaacgcc gccgaaaaag gcgcagcgtt
    15841 aacgctctcc ggcagcacgt ccggcgtcga agcggggcaa accgtcaccg tcacctttgg
    15901 cggcaaaacc tacacctcca cggtggcggc taacggtagc tggagtacca ccgttccggc
    15961 tgcggatatg gcggcgctgc gtgacggcga tgccagcgcc caggtgcggg tgactaacgt
    16021 caacggcaac agcgccacgg caacgcacga atacagcgtc gatagcgccg cgccaacggt
    16081 caccatcaac accattgcca gcgataacat catcaacgcc agcgaagcgg cggcgggcgt
    16141 aacggtgtct ggtaccagca cggcggagac ggggcagacg ctgaccgtca cgcttaacgg
    16201 cactaactac cagaccacgg tgcaggcgga cggcagctgg agcttaacgc tgcccgccag
    16261 cgacttaacc acacttgcca ataacggcta caccctgacc gccacggtca gcgatctggc
    16321 gggtaaccct ggcagcgcca gcaaaggtgt gaccgtcgat accactgcgc cggtgatcag
    16381 ttttaacacc gtggcgggcg atgatgtgat taacaacgtc gaacacactc aggcgcagat
    16441 tatcagcggc accgccacgg gcgcggtggc gggcgaccgc ctggtggtga ccatcgccgg
    16501 gcagcagtat gtcaccagca ccgatgccag cggcaactgg agcgtcggcg tgcctgccag
    16561 cgtgatttcc ggccttgcgg atggcacggt gaccatcagc gccaccatta ccgatagcgc
    16621 aggcaacagc agcacgcaga cgcacaacgt acaggtgaac acggcggcag tgtcgctttc
    16681 ggtcagcacc atcagcggcg ataatattat taacgccgcc gaagcgggca gtgcgctgac
    16741 cctgagcggc actggcacta atttcgcgac aggtacagtg gtgaccgtgt tacttaacgg
    16801 caaaggctac agcgccacca ttcagagcaa cgggagctgg agcgtgaacg tgcctgcggc
    16861 ggatgttgcg gcgctcagtg atggcaccag ctacacggtt agcgcctccg ctcaggacag
    16921 tgccggaaac agcgccacgg cctcgcgcag cgtggcggtg gatctcaccg cgccggttat
    16981 cagcattaac accgtttcga cggacgaccg actcaacgcc gccgaacagc agcagccgtt
    17041 aacccttaac ggctcgacca gcgcggaagt agggcagacg gtcaccgtga cctttggcgg
    17101 caaaacctat accgccacgg ttgccgccaa tggtacctgg gcgctgaacg tgcctgcggc
    17161 ggatctggcc gcgctcgggc agggtgcgca gaccattacc gccagcgtga acgatcgcgc
    17221 gggtaacccc ggtcaaacca cgcacgccct gaccgtcgac accgttgcgc caacggtgac
    17281 catcgccacg gtggcgggtg acgatattat caataacgcc gagcagcttg ccgggcagac
    17341 catcagcggc accaccaccg ccgaagtggg ccagacggtg accgtcacct ttaacgggca
    17401 aacctggacc gcaacggtcg gcagcggcgg aagctggtcg gtgtttattc cggcgcagca
    17461 gtttgccgga ttaagcgacg gcagctacac cattagcgcg acggttagcg atcaggccgg
    17521 aaaccccggc agcgccagcc gtggcgtgac gctgaacggc gacgtgccca ccgtcaccat
    17581 caacaccttt gcgggcgacg atgtggtgaa tgcagcggaa cacggctcat cgctggtgat
    17641 cagcggcacc accaccgcac ccgtcgggca gacgctgacc ctgaccttaa acggcaaaac
    17701 ctacaccacc acggtgcaga cgggcggtag ctggagctac accctcggca gcgccgatgt
    17761 caccgcgctg gcggacggca acgcctacgt gattaacgcc tcggtgagca atgccattgg
    17821 caacaccggt agcagtaatc acaccattac cgtcgatctc agcgctccgg cgatgggcat
    17881 taatatcgat tccctgcaag ccgacactgg ccttagcgcc agcgacttta tcaccagcgt
    17941 cagcccggtg gtggtcaacg gctcgctcac cgccgcgctt gccagtaacg agacggctca
    18001 aatcagtatc gacggcggtt taacctggac cacgcttacc gttaccggca cgacctggcg
    18061 ctataacgac agccgcacgc tgaccgatgg taactatctc tatcaggtgc gggtgattga
    18121 cgcggcgggc aacgtcggtg ccactgacag ccagaacgtg gtgatcgaca ctaccgcgcc
    18181 agatccggca gtgaaaacca tcgccatcag cgcgatcacc actgataccg gattgatcac
    18241 taacgatttt gtcaccagcg acacgacgct tgcggtgagc ggcacgctgg gggcgacgct
    18301 ttctgccggt gagttcgcgc aaatcagcat tgatggcggc accacctggc agaatctttc
    18361 ggtaagcggt ttgacctgga gctatcttga cgggcgtacg ctgaccgatg gcaactacaa
    18421 ctaccaggtg cgggtgatcg acaccgccgg aaatatcggc gcgaccgcca gccagatagt
    18481 cactgtggat accaccgcgc cgctggcgag caaaaccatc gccattgcga gcatcagcga
    18541 tgacaccggg ttaagcagca gcgattttgt tacacgcgac accacgctga ccgtgcgcgg
    18601 cacgctcggc gcggcgcttg ccgccgatga acgggcgcaa atcagcctcg acggcggcgt
    18661 cacctggact acgttaaccg ttatcggcac cagctggagc tatgccgatg gtcgcacgct
    18721 tactgacggc acctggaact acacggtgcg ggtggtggat ctggcgggga acgttgggca
    18781 gaccgcaacg caaaacgtgg tggtcgacac caccagcccg gaagcggcga aaagtatcac
    18841 cattaccggc atcagcgatg acaccggaac cagcagcagc gattttatta ccagcgacac
    18901 cacgcttacc gtgcgcggcg tgttgggcgc ggcgctcggc gctaatgagt tcgcgcaaat
    18961 cagtaccgac aacggcgcaa cctgggtgaa cgtgaccgtt gccgcagacg gccttaactg
    19021 gagttacgtt gacgggcgca cgctcaccaa cggcaccacc acctggcagg tgcgggttgt
    19081 cgatctggcg ggcaacgttg gcgcaaccgg cagccagtcg gcgcagattg acaccgttaa
    19141 cccggcgcag gtgctcacca tcgccagcat tagcaccgac acggggagtt cggcaactga
    19201 ctttatcacc agcgatacca cgctcacgct gactggttcg ctgggggcgg ggcttgccag
    19261 cggcgaagtg gcgcagatta gccttgatag cggcgcgacc tggatcaccc tcaccactaa
    19321 cggcacgcag tggacttaca ccgacggccg cacgctgacc gacggcagct acgtttatca
    19381 ggtgcgggtg ctggatctgg cgggtaacac cggcccggtg gtatcaaaaa cggtggtagt
    19441 tgatacgatt aaccccaccg ccacgccaac tattgtgtcg tataccgatg atgtcgggca
    19501 gcggcaggga acgttaagca gttcgcaggc cactgacgat accacgccgc tgttaaacgg
    19561 cgtgctctct gcgccgcttg ccagcggtga agtggtctat ctctaccgta acggcctgct
    19621 gttaggtgcg gtgacgatgg tcggcgcgct gaactggacc tacagcgaca gcgggctggt
    19681 tagcggtgcc tatacctaca gtgcgcgggt agtggatctg gcggggaata tcacctcctc
    19741 cagtgatttt gtcctgacgg tcgatacctc tattccgacc acgctggcgc aaatcaccag
    19801 ccagaccacg cgcgatacta cgccgattat cagcggggtg atcaccgccg cgcttgccag
    19861 tgggcagtat gttgaagtgg tgatcaacgg caaaacctac acctctgaac cgggcggcgc
    19921 ggtagtggtc gatccggcgc acaacacctg gtatgtacag ttgccggata acgatgggct
    19981 gacagtttcc gcgaccgcct ataccgttac tgcgcaggta aaaagttcgg cgggtaacgg
    20041 caataacgcc aatattagca acggcacggt gacggttaac gcggcgattg attacacacc
    20101 gacctggacc accgccagca aaaccaccgc ctgggggttg acctacggcc tcgactcgca
    20161 cgggatgtgg acggtgctgg caaaccagca ggtaatgcaa tcgactgacc cactcacctg
    20221 gtcgaagacc gcgctgacgc tgtatcagag cggcaacaac tacgccacca gctccatcgc
    20281 cgattacgac cgtaacggca cgggcgatct gtttatcact cgtgatgact acggtacagg
    20341 ctatatcaac ggctttacca ataacggcga cggcaccttt tccagcgcta ttcaggtcac
    20401 cgtcggcacc ctgacgtggt acggctcgat tgtggcattt gataaagagg gcgacggcta
    20461 tctcgacttc tggattggcg acgcgggcgg gccggactcc aacaccttcc tgtggaacaa
    20521 cgcaggtacg ctggtaggta actccaccac gtcgaacagc ggcggtagcg ccacggtggg
    20581 cggggcggtg acggggtatc tttcgctcaa cgaaggttct ggcgtcgatc tgaacaatga
    20641 cggcaggatc gacctggttc agcacaccta caacctgaac aactattaca cgctatcttc
    20701 gctcatcaac caggggaatg ggacgtttgt ctgggggcag aacaccacca ataccttctt
    20761 gagcggggcg ggtagtggcg ctatgagcag cagcgtttcc atgacctggg ccgatttcga
    20821 tggtgacggc gatatggatc tcttcctgcc cgccagccag ggaagagcta actacggctc
    20881 gctgttattc aacaccaacg gcgtactggg tagcccggtg gcggtgggcg caacggcaac
    20941 cacctacgcc agccagttta gcctggcggt ggactggaac catgacggcc tgatggatat
    21001 cgcccgtatc gcccagaccg gacaatcgta tctttacacc aacgttggtg gtgccagcaa
    21061 ctggacacaa tcagccctcg gcggcagcca gagcggtacc accagcggcg tggcggcaat
    21121 ggactacgac tgggacggcg cggtggatgt gctggtgacc aaacagtcgg gcagcgtgtt
    21181 cctgatccgc aacaccaaca cggtgagcta cggcacttcg ctacacctgc gcatcaccga
    21241 tcccaacggc attaacgtct attacggcaa taccgtgaag ctgtacaact cggcgggagt
    21301 gctggtcgcc acgcaaatta ttaacccgca gtcgggtatg ggggttaacg acacctcggc
    21361 gctggtcaac ttctacgggc tgaatgccgg agaaacctac aacgcggtgc tgatcaaatc
    21421 caccggcacc accgccagca atatcgacca gacagtcaat accacctggg gcggtttaca
    21481 ggcgacagat gccacccacg cttacgacct cagcgccgaa gcaggtacgg cgagcaataa
    21541 cggcaagttc gtcggcacgg gctataacga caccttcttt gccaccgcag gcaccgacac
    21601 ttacgacggt tccggcggct gggtgtacag ctccggcacc ggaacgtggc tggcgaacgg
    21661 cgggatggac gtggttgatt tccggctttc gacggtgggc gtgaccgcga acttaagcag
    21721 cactgccgcg caggccaccg gctttaacac ctcgacattt accaatatcg aaggtatttc
    21781 cggctcgaat tttaacgaca ctctgaccgg cagcagcggc gataaccaac tggaagggcg
    21841 cggcggcaac gacacgctca acatcggcaa cggcggccac gacaccttgc tctataaact
    21901 gctcaacgcc agcgacgcca ccggcggcaa cggatcggat gtggtgaatg gttttacggt
    21961 aggcacctgg gaaggcaccg ccgacaccga tcgcattgat atccgcgagc tgttgcaggg
    22021 cagcggctac accggcaacg gcaaagccag ctacgtcaac ggtgtggcga cgctggatgc
    22081 gcaggccgga aacatcggtg attttgtcaa agtcatccag agcggcagcg acaccatcgt
    22141 gcagatcgac cgcgacggca cgggcggcac ttttgcggca actaacgtgg tcacgctgac
    22201 gggcgtgcat accgacctcg ccactttgct ggcgaatcat cagttgatgg tggtgtagat
    22261 aatgaaaaaa aatgcccagt ccgtggaagc ctggctggag gcgatgatcg ccgtggcgcg
    22321 ctattatcgc cttgattttt ctcaggagaa cgtgcgcgca accgtcaact gggagcgcga
    22381 cagcaagcgg gaagagttgc ttaccgatat ggcgcggcag ttggggatgg ggctgcggtt
    22441 ggtggagttt tccgccgact cgcttaaccc ctggcggctg ccgctgatcg cggtgtttga
    22501 taatcaacag attggcgtga tcacccgccg cgacaattac gacaatatca gcgttcagtt
    22561 cagcggtgac gaagggctgg aaaccacgct taatatcgcg gatattgaag acaaaatcgt
    22621 cgaactggcg ctgctgcgcc cgctcagcgc catccctgac gcgcgggtgg atgactatat
    22681 tcgcccgtat caggcgaact ggttctggag tctgtcgcta aaagactggc ggcgctatgg
    22741 cgatattatg ctggcctcac tggtggcgaa cgtgctggcg ctggcggcga tgattttctc
    22801 gatgcaggtt tacgaccggg tggttccggc gcaatcctac ccgacgctgt gggtgctgtt
    22861 cgccggggta atgatggcga tcctgtttga gttctgtatg cgcatggtgc gcacgcatct
    22921 ctcggatatg attggcaagc gggcggatct gcgcatttcg gaccgcgttt ttggtcatgc
    22981 gctacggctg aaaaacaacg tgcgatcgaa atccaccgga tcgtttatct cacagatccg
    23041 cgagctggaa tcagtgcggg agcttattac gtccaccacc atcggcgcgg tggcggacct
    23101 gccattcttc ctgctgtttg tttttatttt gtggatgatc ggcggctggc tggtgctggt
    23161 ggttctgctg gcgctgccgt tattggtgat ccccggcctg ctggtgcaac gcccgctggc
    23221 gcggctggcg aacgaaggga tgcgcgagtc ggcggtgcgt aatgctacgc tggtggaagc
    23281 cgtgcagtcg attgaagaca ttaagctgct gcgcgccgaa cagcgttttc agaatcagtg
    23341 gaaccacacc aacgacgtgg cctcgtccat cagcatgaag caacgttttc tcactgggct
    23401 gctgctcacc tggacccagg aagtgcaatc catcgtctac gtggtggtgc tgctggtggg
    23461 ctgctttatg gtgatgaacg gcgacatgac caccggtgcg ctggtcggga cgacgatcct
    23521 ggcttcgcgt accatcgcgc cgctgtcgca gatatccggc gtgctctcgc gttggcagca
    23581 ggcaaaagtg gcgcgcaacg gccttgatga actgatgaag cgccccgtgg atcagccgga
    23641 acacggcaaa ctggtgcata aagcggtgct gcacggcaat tatcagttca gcaacgcggt
    23701 gttttattac gacgaagaag agaaaattgc cgatgtggcg atcggcaaac tcaacattca
    23761 ggccggagag aaaatcgcta ttctcgggcg caacggcgcg ggtaagtcaa cgctgctgca
    23821 aatgctcgcc ggaatgcgta tcgcccaaca ggggcaggtg ctgctggata acatcagcat
    23881 cgggcaactt gatccggcgg atctgcgccg cgacatgggg ctactcagcc agaccgggcg
    23941 actgttcttt ggttcgctgc gggaaaacct caccatgggg atgccggagg ccagtgatga
    24001 agatatcgaa cgcgcgttaa ccctcagcgg ggcgctgccc tttgtgcaga agcagaaaaa
    24061 cggcctcaac tacatgattc aggaaggggg atttggcctc tctggtggtc agcggcaaac
    24121 gctgctgctg gcgcggctac tcatctctca acccaatatc gtgctgctcg acgaacccag
    24181 cgcctcgctg gacgaaatgg cagaagcgca tttaatcgaa caactgaaac agtggattgg
    24241 gcatcgcacg ctgattatcg ccactcaccg cacggcgatg ctgcagctgg tcgacaggat
    24301 aatcgtgatg gatcaggggc gcattgtgat ggacggcgcg aaagaggcca ttttgcgtga
    24361 acagggcgag cccacggcgc gacgggtggt gttgcaggag aagaataagg ggtctgccgc
    24421 atgaacggac aactgcaata taaccgtggc ctgactgaac cccgcctgcc gcgttcggcg
    24481 ttggcggtac gcgttaccgc tgtgatgctg ctctgttttc ttggctgggc gtggtacttc
    24541 cagcttgatg aagtcaccac cggcagcggc acggtagaac cctccgggcg cgaacaggtg
    24601 gtgcagtcgc tggaaggcgg aattctgtat catctcgacg tcaaggttgg cgatattgtt
    24661 gaacagggcc agccgctggc gcagcttaac cgcaccaaaa cggaatccga cgtccaggaa
    24721 gcgatgtcgc ggctgtacgc ggcgctggca acctcggcgc ggctgcgggc ggaagtgagt
    24781 aataaaccgc tggtcttccc tgacgaactg aataaattcc ccgaacttat cgagtcggaa
    24841 acggcgttgt acaacactcg ccgcgacggg ttgaacaaag ccaccaccgg gctgacgcag
    24901 gggattagcc tggtcaaccg tgaactggcg atgacccagc cgctggtgaa acagggggcg
    24961 gcgagcagcg tcgaagtgct gcgcctgcaa cgccaggcca atgaactgga aaacaaactc
    25021 agcgacgtgc gcacgcaata ctacgttcag gcgcgcgaag agttagcaaa agccaatgcg
    25081 gaagtggaaa cccagcgttc ggtgatccgt ggacgggaag actctctcac caggctcaac
    25141 tttactgcgc cggtgcgagg cattgttcag gatattgatg tcaccacggt tggcggcgtc
    25201 attgcccccg gtggtaaact gatgaccatc gtgccgctgg atgagcaact gctgattgag
    25261 gcaaaaatct ccccgcgcga tgtggcgttt atccatccgg ggcaaaaatc gctggtaaaa
    25321 ataactgcct acgattactc catctacggc ggcttgccgg gggaagtggc ggtgatttca
    25381 ccggataccg tgcaggatga agtgcggcgc gatgtttatt actatcgggt gtacattcgc
    25441 actttcagca accatctgga aaacaaaagc aaacagcagt ttccgatatt ccccggtatg
    25501 gtggcgacgg tcgatatccg caccggcaaa aaaagcgtgc tggattatct gttgaaaccc
    25561 tttaacaaag cacaggaggc gttgcgcgaa cgatgaacat tagcgatgta gcaaaaatta
    25621 ccggcctgac cagcaaagcc attcgcttct atgaagagaa ggggctggtg acgccgccga
    25681 tgcgcagcga aaatggttat cgaacctaca cgcagcagca tctcaacgaa ttgaccttac
    25741 tgcgccaggc gcgccaggtg ggctttaacc tggaagagag cggcgagctg gtaaatctgt
    25801 ttaacgaccc gcagcgtcac agtgccgacg tcaaacgtcg tacgctggag aaggtggcgg
    25861 agatcgaacg gcatatcgaa gaactgcaat ctatgcgcaa ccagctactg gcgctggcga
    25921 atgcctgccc cggtgatgac agcgccgact gcccgattat cgaaaatctc tctggctgct
    25981 gtcatcatcg ggcggggtga aaatcagtga atccccgttt gcaccgatag aatatgctgc
    26041 aaactttcta tcataatgcg ccccgactga aataagcctt cctggatttc gttgttaaca
    26101 ttcacagcgt gaaccaggga ggctaataat actacttgca agaaggcaaa aataaacgca
    26161 ccgataatca taatatgcgt caggggtatt ttcctttgct ccttcatttt aaataaccca
    26221 caaactggcc ttctttcatt aaggcgactt ttcctgtgtg atgataaaca atgacgttat
    26281 atagacctga ctgataaaca gtggcatctt tattgtcttc atcaattgca cataatgaat
    26341 cattgagata agcaaagttt ttactgctca ttctaaaggt catgccaaat cctttataaa
    26401 tggctttctt tgccgcctgg gcgtgttgtg cggcttctgc atttttaaat gcctgcgctt
    26461 cggaagcact taaaatagcc gtctggcgct gctgttctgc gagataacag gtgtcatggc
    26521 tgacgccctg cgcctggcaa tctgcgatgc gggaagcggg tgagctacag ccagccagta
    26581 atataaccgc cagcagggaa aacgatttaa tggagctatt catcattgtt aaccttaaat
    26641 aatgtacaaa ttaatgaaga ggtgaaaacc ggcaagcttc cttgctctta tttatattga
    26701 ggattgaaat attagaacga atatcccacg ccgacacccg cgccgaaatc agattgtgta
    26761 tctgcagaga caccggcttt aatcacggta tgcgttccgg cattaaaaga tgttcctaca
    26821 gacaccgcag attcaccgtt gaaggttccc gctcccgctg acaccatcat cacgtcaccg
    26881 gttttaacct gtggttgtga ggcgatagcg atggcagatg cagttcccgc attggcttct
    26941 ttacgattgt catcaacttc gttttttagc gaactgaaat ggttgttgag cgtgttaatt
    27001 tgctggctgt tatctgcaac ccgtttttgg gtgttggtta actgttggtg cgtttgggtt
    27061 acgtcttgtg acaggctgtc aatttgttca tgtgcctggg cagactcctg cgccagactt
    27121 tgtatttgtt tatcgtattg agtcctttca ttttgctgtt gtactgccac atcggttgtc
    27181 agcgcattca ttttctgccc ttgttcagtg gtagtgcgct gattagcctc attttgagcc
    27241 gccagtctgg cattaatcat ctcctgataa tgcgcggtat tttgcgtatc tccagtcgcg
    27301 gcgagcgttt tttgtgtggc tacgatctgc gcctgttgct ctgtaatttg cgtttgctgg
    27361 gcgtcagtta ccgcagactg tcgggcgttg atcatgctct gataacgagc agttgtctgt
    27421 acatcacctg tagcggcaac ggttttttgt cctgcgtcag gatctgtctg gacagtggga
    27481 gtctgagtgg tatcaggtgt ttcagaaggg atgtagtgat gttcagtcaa ataggtatac
    27541 gcagcggggc tgttgtctgt gcgttctaaa aagcgggtaa tcttctgctc aatctccgat
    27601 tgcgacatgc cagagttaat ggctgaaagc tctgttgccg ccgcttcaat ggtatcacca
    27661 gctaaaacaa cagggctgga tgttgctgaa attatgagtg ccagtaaagc tacgtttaca
    27721 gttttcattc attttctctt gttcaaaata agtcgtatta atgtttctat
//
LOCUS       DAPGOF010000054        27546 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.054, whole genome shotgun sequence.
ACCESSION   DAPGOF010000054 DAPGOF010000000
VERSION     DAPGOF010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.054"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(91..1341)
                     /gene="icd"
                     /locus_tag="RHV13_001977"
     CDS             complement(91..1341)
                     /gene="icd"
                     /locus_tag="RHV13_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HDV1241510.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGEAIIENM"
     gene            1513..2166
                     /gene="rluE"
                     /locus_tag="RHV13_001978"
     CDS             1513..2166
                     /gene="rluE"
                     /locus_tag="RHV13_001978"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HDV1241511.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2176..2637
                     /gene="nudJ"
                     /locus_tag="RHV13_001979"
     CDS             2176..2637
                     /gene="nudJ"
                     /locus_tag="RHV13_001979"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HDV1241512.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2691..3797
                     /gene="mnmA"
                     /locus_tag="RHV13_001980"
     CDS             2691..3797
                     /gene="mnmA"
                     /locus_tag="RHV13_001980"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDV1241513.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3833..4474
                     /gene="hflD"
                     /locus_tag="RHV13_001981"
     CDS             3833..4474
                     /gene="hflD"
                     /locus_tag="RHV13_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HDV1241514.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4478..5848
                     /gene="purB"
                     /locus_tag="RHV13_001982"
     CDS             4478..5848
                     /gene="purB"
                     /locus_tag="RHV13_001982"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDV1241515.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVASFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6016..6687
                     /gene="phoP"
                     /locus_tag="RHV13_001983"
     CDS             6016..6687
                     /gene="phoP"
                     /locus_tag="RHV13_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HDV1241516.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6687..8147
                     /gene="phoQ"
                     /locus_tag="RHV13_001984"
     CDS             6687..8147
                     /gene="phoQ"
                     /locus_tag="RHV13_001984"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HDV1241517.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSTPKDE"
     gene            8223..9344
                     /gene="roxA"
                     /locus_tag="RHV13_001985"
                     /gene_synonym="ycfD"
     CDS             8223..9344
                     /gene="roxA"
                     /locus_tag="RHV13_001985"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HDV1241518.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVEPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9490..10719)
                     /gene="pepT"
                     /locus_tag="RHV13_001986"
     CDS             complement(9490..10719)
                     /gene="pepT"
                     /locus_tag="RHV13_001986"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDV1241519.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELAIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRKS"
     gene            10969..12105
                     /gene="potA"
                     /locus_tag="RHV13_001987"
     CDS             10969..12105
                     /gene="potA"
                     /locus_tag="RHV13_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HDV1241520.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            12089..12946
                     /gene="potB"
                     /locus_tag="RHV13_001988"
     CDS             12089..12946
                     /gene="potB"
                     /locus_tag="RHV13_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HDV1241521.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            12943..13737
                     /gene="potC"
                     /locus_tag="RHV13_001989"
     CDS             12943..13737
                     /gene="potC"
                     /locus_tag="RHV13_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HDV1241522.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            13734..14780
                     /gene="potD"
                     /locus_tag="RHV13_001990"
     CDS             13734..14780
                     /gene="potD"
                     /locus_tag="RHV13_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HDV1241523.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            14838..15299
                     /locus_tag="RHV13_001991"
     CDS             14838..15299
                     /locus_tag="RHV13_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HDV1241524.1"
                     /translation="MSQDSKVFFRIFFGIGLVLILISVVIFYNQFTYSKDAIHTEGVI
                     VDTVLHSSHSHRTGKNGSWYPVVAFRPTPDYTLIFNSSIGSDFYEDSEGDKVNVYYSP
                     GHPEKAEINNPWVNFFKWGFIGIMGVIFIAVGLLISMPSSKKSRRKRKSRP"
     gene            15296..16081
                     /locus_tag="RHV13_001992"
     CDS             15296..16081
                     /locus_tag="RHV13_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDV1241525.1"
                     /translation="MKKIIILLSLLILLPLTVTSKPLIPIMKTLFTDVTGTVPDAEEI
                     ARKAELFRQQTGIAPFIVVVPGVNEASFRQNGKAMLAHASSSLSDVKGSVLLLFTTRE
                     PRLIMITNGHVESSMDDKHLGLLVENHTLAYLHADLWYQGINNALAVLQAQILKQPTP
                     PLTYYPHPGQQQENDPPGSTTTLGLFAWAVAFIVFTAFFNYTTRLNYALKFAVAMTIA
                     NMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLLTSKR"
     gene            complement(16218..17039)
                     /gene="cobB"
                     /locus_tag="RHV13_001993"
     CDS             complement(16218..17039)
                     /gene="cobB"
                     /locus_tag="RHV13_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDV1241526.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWIGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(17055..17966)
                     /gene="nagK"
                     /locus_tag="RHV13_001994"
     CDS             complement(17055..17966)
                     /gene="nagK"
                     /locus_tag="RHV13_001994"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HDV1241527.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQLEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(17995..19239)
                     /gene="lolE"
                     /locus_tag="RHV13_001995"
     CDS             complement(17995..19239)
                     /gene="lolE"
                     /locus_tag="RHV13_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HDV1241528.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDHVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(19239..19940)
                     /gene="lolD"
                     /locus_tag="RHV13_001996"
     CDS             complement(19239..19940)
                     /gene="lolD"
                     /locus_tag="RHV13_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HDV1241529.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLDHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(19933..21132)
                     /gene="lolC"
                     /locus_tag="RHV13_001997"
     CDS             complement(19933..21132)
                     /gene="lolC"
                     /locus_tag="RHV13_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HDV1241530.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            21394..22467
                     /locus_tag="RHV13_001998"
     CDS             21394..22467
                     /locus_tag="RHV13_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HDV1241531.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMLTASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            22595..26041
                     /gene="mfd"
                     /locus_tag="RHV13_001999"
     CDS             22595..26041
                     /gene="mfd"
                     /locus_tag="RHV13_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDV1241532.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLENSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKHW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            26185..27147
                     /gene="ldtC"
                     /locus_tag="RHV13_002000"
     CDS             26185..27147
                     /gene="ldtC"
                     /locus_tag="RHV13_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HDV1241533.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(27230..27487)
                     /gene="bhsA"
                     /locus_tag="RHV13_002001"
     CDS             complement(27230..27487)
                     /gene="bhsA"
                     /locus_tag="RHV13_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HDV1241534.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         6707 a         7138 c         6979 g         6722 t
ORIGIN      
        1 aaatatgctc gatatgctat taataatatc cacataaata aaacaacggg cgtgttatac
       61 gcccgtttca atatttaact catggagaga ttacatgttt tcgatgatcg cttcaccaaa
      121 ctctgaacat ttcagcagtt tagcgccttc catcagacgt tcgaagtcat aggttacagt
      181 cttcgcgttg atcgcgcctt ccataccttt aacaatcagg tctgcggctt cagtccaacc
      241 catgtggcgc agcatcatct ctgcagagag aataatagag cctgggttta ctttgtcctg
      301 accggcatat ttcggcgcag taccgtgggt ggcttcaaac agggcgcatt cgtcaccgat
      361 gtttgctcca ggggcgatac cgataccgcc aacctgcgct gccagagcgt cagaaatgta
      421 gtcaccgttc aggttcatac aggcgataac atcatattca gccggacgca gcaggatctg
      481 ttgcaggaat gcatcagcaa tcacgtcttt aatgacgatc tctttgccgg tattcgggtt
      541 tttaactttc agccacgggc cgccgtcgat cagttcaccg ccaaattctt cacgcgccag
      601 ctggtagccc cagtctttaa acgcgccttc ggtgaacttc atgatgttgc ctttgtgcac
      661 cagggtcaca gagtcacgat cgttggcaat tgcgtattcg atcgctgcac gaaccagacg
      721 tttggtgcct tcttcagaac acggcttaat accgataccg caatgttccg ggaagcgaat
      781 tttcttcacg cccatctctt cacgcaggaa tttaatcact ttctcagcgt cggcagagtc
      841 agctttccat tcgatacctg cataaatgtc ttccgagttt tcacggaaga taaccatatc
      901 ggtcagttca gggtgtttaa ccgggcttgg agtgccctga tagtaacgta ccggacgcag
      961 gcagatgtag agatccagtt cctggcgcag ggcaacgttc agagagcgaa taccgccacc
     1021 aacaggagtg gtcagcggac ctttaatggc aacgcgatat tcacgaatca gatcaagagt
     1081 ttcagcaggc agccagacgt cctgaccata aacctgtgtg gatttttcac cggtgtaaat
     1141 ttccatccag gagattttac gctcgccttt ataggctttc tcgactgcag cgtcgaccac
     1201 tttcagcatg gctggggtta catctacacc gattccatca ccttcaatgt aagggataat
     1261 cggattttca ggaacgttga gtttgccgtt ttgcagggtg atcttcttgc cttgtgccgg
     1321 aacaactact ttactttcca ttcacctctc cttcgagcgc tactggtttg ctcgtctgcc
     1381 accacgttgc atatgcgttt gcgtcctgcg atacggatgc tttagagcaa ttttttgtta
     1441 atgatttgta attggcttgt caatactacc ctattgtttg ccgtcatgaa agatgcgcgt
     1501 tattaggcta taatgcggca attcataatc tctgaaaata ccatgcaaaa aacttctttt
     1561 agaaatcacc aggttaagcg attcagctcg caacgttcta ccaggcgtaa acctgaaaac
     1621 cagcccacgc gtgtgatcct gttcaataaa ccctacgatg ttcttccgca gttcaccgat
     1681 gaagccggac gcaaaacatt aaaagaattc atcccggttc agggcgttta tgcagcaggt
     1741 cgtcttgacc gcgatagcga agggttactg gtgctgacca ataacggcgc gttgcaggcg
     1801 cgtttaaccc agccgggcaa acgcaccggg aaaatctatt atgtgcaggt ggaaggtatt
     1861 cccacacaag acgcacttga agccttgcgc aatggcgtaa ccttaaatga tggccctacc
     1921 ctgcccgccg gcgcggagct ggttgacgaa cctgcgtggt tatggccgcg gaatccacca
     1981 attcgtgaac gcaaaagtat tcccaccagc tggctgaaga tcaccttata tgaaggacgt
     2041 aatcgccagg tgcgccgcat gaccgcccat gttggtttcc ccacgctgcg actgattcgc
     2101 tatgcgatgg gtgattactc tttggataat cttgctaatg gcgaatggcg agaagtgaca
     2161 gattaaggat gcaaaatgtt taaaccgcac gttaccgttg cttgcgtggt gcacgcagaa
     2221 ggcaaatttt tagtcgttga agagacgatt aatggtaaag cgttatggaa ccaaccggcg
     2281 gggcatctgg aagcggatga aaccttagtg gaagccgccg cccgtgagct gtgggaagaa
     2341 accggcatca gcgcgcagcc gcaacacttt attcgtatgc atcagtggat tgcgccagat
     2401 aaaacgccgt ttttgcgttt cctctttgcc attgagcttg agcaaatatg cccgacccag
     2461 cctcatgaca gcgatatcga ctgctgtcgt tgggtcagcg ccgaagaaat tttaaaggcg
     2521 tcaaatcttc gttcgccgct ggtggcggaa agtatccgtt gttatcaaag cgggcaacgc
     2581 tatccgctgg agatgattgg cgattttaac tggcctttta caaagggtgt catctaaaag
     2641 gctgcgtgat agaatacgcc gccttgaagt tcaatgtcgt gagtgatcca atgtctgaaa
     2701 ccgcaaaaaa agtaatcgtc ggcatgtccg gcggtgtcga ttcctccgtt tctgcctggc
     2761 tgttgcaaca acagggatat caggttgaag gcctgtttat gaagaactgg gaagaagacg
     2821 acggtgagga atattgcaca gcggcagcgg atctggctga tgcccaggct gtctgcgaca
     2881 agctcggcat tgaactgcac accgttaact ttgctgccga gtactgggac aacgtcttcg
     2941 aactgttcct tgccgaatat aaagccggtc gcacgccgaa tccggatatt ctgtgcaaca
     3001 aagagatcaa atttaaagcc ttcctcgaat ttgccgccga agatttaggt gccgattata
     3061 tcgctaccgg tcattacgta cgtcgggccg atgtcgatgg caagagccgc ctgctgcgtg
     3121 gtctggacag caataaagac cagagctact tcctttatac gctcagccat gagcagattg
     3181 cgcaaagcct gttcccggtc ggcgaactgg aaaaaccgca ggtgcgtaag attgctgaag
     3241 atcttggtct ggtcaccgcg aagaaaaaag actctaccgg catttgcttc attggcgaac
     3301 gtaaattccg cgagttcctg ggccgttatc tcccggcgca accgggcaaa atcattaccg
     3361 tcgatggcga tgaaattggt gagcaccagg ggctgatgta tcacactctc ggtcagcgta
     3421 aaggtctggg tatcggtggc accaaagaag gtaccgaaga accgtggtat gtggtggaca
     3481 aagacgtcga aaacaacatt ctggttgttg ctcagggcca tgaacacccg cggctgatgt
     3541 ctgtcgggtt gattgcccag cagttgcact gggtcgatcg cgaaccattc accggcacta
     3601 tgcgttgcac ggtaaaaacc cgctatcgcc agaccgatat cccttgcacc gtcaaggcgc
     3661 tggacgatga tcgcattgaa gtgattttcg atgaaccggt tgccgccgtg acgccgggcc
     3721 agtctgccgt cttctataac ggtgaagtgt gcctcggtgg cggtattatt gagcagcgtc
     3781 tgccgctgcc ggtctgatta ttgtctttaa ttacaacaag gaagcagtga acgtggcaaa
     3841 gaattactat gacatcaccc tcgccctggc cggtatttgt cagtcggcac gcctggtgca
     3901 acaactcgct caccaggggc attgtgatgc cgatgcgcta cacgtctcac tcaacagtat
     3961 tattgatatg aaccccagct cgacgctggc ggtttttggc ggtagcgaag ccaacctgcg
     4021 cgtcgggctg gaaaccctgc tcggcgtgct caatgccagc agtcgccagg gcttaaacgc
     4081 tgagttaacc cgctacacac tcagcttgat ggtgcttgag cgcaaactct cctcagcgaa
     4141 aggcgcgctc gacactctgg gcaaccggat caacggcctg caacgccagc tcgaacactt
     4201 cgatttacag tccgaaacgc tgatgagcgc gatggctgct atctatgttg atgtgatcag
     4261 cccgcttggc ccgcgcattc aggtcaccgg ttcccctgct gtactgcaaa gcccacaagt
     4321 gcaggcgaaa gttcgcgcaa ccctgctggc aggcattcgc gccgccgtgc tctggcacca
     4381 ggtcggcggc ggacgtctgc aactgatgtt ttctcgtaat cgcctgacca ctcaggcaaa
     4441 acaaattctt gctcatttaa ccccggagtt gtgatctatg gaattatcct cactgaccgc
     4501 cgtttcccct gtcgatggac gctacggcga taaagtcagc gcgctgcgcg ggattttcag
     4561 cgaatatggt ttgctgaaat tccgtgtaca agttgaagta cgttggctgc aaaaactggc
     4621 cgcgcacgca gcgatcaagg aagttcctgc ttttgctgcc gacgcaatcg gttaccttga
     4681 tgcaattgtc gccagtttca gcgaagaaga tgcagcacgc atcaaaacca tcgagcgtac
     4741 tactaaccac gacgttaaag cggttgagta tttcctgaaa gaaaaagtgg cggagatccc
     4801 ggaactgcac gcggtttctg aattcatcca ctttgcctgt acttcggaag atatcaataa
     4861 cctctcccac gcattaatgc tgaaaaccgc gcgtgatgaa gtgatcctgc cgtactggcg
     4921 tcaactaatt gatggcatta aagatctcgc cgttcagtac cgcgatatcc cgctgctgtc
     4981 tcgtacccac ggtcagccag ccacgccgtc aaccatcggt aaagagatgg caaacgtcgc
     5041 ctaccgtatg gagcgccagt accgccagct taaccaggtg gagatcctcg gcaaaatcaa
     5101 cggcgcggtc ggtaactata acgcccacat cgccgcttac ccggaagttg actggcatca
     5161 gttcagcgaa gagttcgtca cctcgctggg tattcaatgg aacccgtaca ctacccagat
     5221 tgaaccgcac gactacattg ccgaactgtt tgattgcgtt gcgcgcttca acaccattct
     5281 gatcgacttt gaccgtgacg tctggggtta tatcgccctt aaccacttca aacagaaaac
     5341 catcgccggt gagattggtt cttccaccat gccgcataaa gttaacccga tcgacttcga
     5401 gaactccgaa gggaatctgg gcctttccaa cgcggtattg cagcatctgg caagcaaact
     5461 gccggtttcc cgctggcagc gtgacctgac cgactccacc gtgctgcgta acctcggtgt
     5521 gggtatcggt tatgcgctga ttgcgtatca atccaccctg aaaggcgtga gcaaactgga
     5581 agtgaaccgt gaccatctgc tggatgaact ggatcacaac tgggaagtgc tggctgagcc
     5641 aatccagaca gttatgcgtc gctatggcat cgaaaaaccg tatgagaagc tgaaagagct
     5701 gactcgcggt aagcgcgttg acgccgaagg catgaagcag tttatcgacg gtctggcgtt
     5761 gccagaagaa gagaaagccc gcctgaaagc gatgacgccg gcaaactaca ttggtcgcgc
     5821 catcaccatg gttgatgagc tgaaataaac tcgtatcagt gccggatggc gatgctgtcc
     5881 ggcctgctta ttaagattat ccgcttttta ttttttcact ttacctcccc tccccgctgg
     5941 tttatttaat gtttaccccc ataaccacat aatcgcgtta cactatttta ataattaaga
     6001 cagggagaaa taaaaatgcg cgtactggtt gttgaagaca atgcgttgtt acgtcaccac
     6061 cttaaagttc agattcagga tgctggtcat caggtcgatg atgcagaaga tgccaaagaa
     6121 gccgattatt atctcaatga acatttaccg gatattgcga ttgtcgatct cggattgcca
     6181 gacgaggacg gtctgtcact gattcgccgc tggcgtagca atgatgtttc actgccgatt
     6241 ctggtattaa ccgcccgtga aagctggcag gacaaagtcg aagtattaag tgctggtgct
     6301 gatgattacg tgactaaacc atttcatatt gaagaggtga tggcgcgaat gcaggcatta
     6361 atgcggcgta atagcggcct ggcttcacag gtcatttcgc tccccccgtt tcaggttgat
     6421 ctctctcgcc gtgaattatc tattaatgac gaagtgatca aactgaccgc gttcgaatac
     6481 accattatgg aaacgttgat acgtaataat ggcaaagtgg tcagcaaaga ttcgttaatg
     6541 ctacaactct atccggatgc ggagctgcgg gaaagccata ccattgatgt actgatggga
     6601 cgtctgcgca aaaaaattca ggcacaatat ccgcaagaag tgattactac cgttcgcggc
     6661 cagggctatt tgttcgaatt gcgctgatga aaaaattact gcgtcttttt ttcccgctct
     6721 cgctgcgggt acgttttctg ttagcaacgg cagcggtagt actggtgctt tcgcttgcct
     6781 acggaatggt cgcgctgatc ggttatagcg tcagtttcga taaaactacg tttcggctgt
     6841 tacgtggcga gagcaatctg ttctataccc ttgcgaagtg ggaaaacaat aagttgcatg
     6901 tcgagttacc cgaaaatatc gacaagcaaa gccccaccat gacgctaatt tatgatgaga
     6961 acgggcagct tttatgggcg caacgtgacg tgccctggct gatgaagatg atccagcctg
     7021 actggctgaa atcgaatggt tttcatgaaa ttgaagcgga tgttaacgat accagcctct
     7081 tgctgagtgg agatcattcg atacagcaac agttgcagga agtgcgggaa gatgatgacg
     7141 acgcggagat gacccactcg gtggcggtaa acgtctaccc ggcaacatcg cggatgccaa
     7201 agttaaccat tgtggtggtg gataccattc cggtggagct aaaaagttcc tatatggtct
     7261 ggagctggtt tatctatgtg ctctcagcca atctgctgtt agtgatcccg ctgctgtggg
     7321 tcgccgcctg gtggagttta cgccccatcg aagccctggc aaaagaagtc cgcgaactgg
     7381 aagaacataa ccgcgaattg ctcaatccag ccacaacgcg agaactgacc agtctggtac
     7441 gaaacctgaa ccgattgtta aaaagtgaac gcgaacgtta cgacaaatat cgtacaacgc
     7501 tcaccgacct gacccatagt ctgaaaacgc cactggcggt gctgcaaagt acgctgcgtt
     7561 ctctgcgtag tgaaaagatg agcgtcagtg atgctgaacc ggtaatgctg gagcaaatca
     7621 gccgcatttc acagcaaatt ggctactacc tgcatcgtgc cagtatgcgc ggcgggacat
     7681 tgctcagccg cgagctgcat ccggtcgccc cactgctgga caatctcacc tcggcgctga
     7741 acaaagtgta tcaacgcaaa ggggtcaata tctctctcga tatttcgcca gagatcagct
     7801 ttgtcggtga gcagaacgat tttgtcgagg tgatgggcaa tgtgctggat aatgcctgta
     7861 aatattgcct cgagtttgtc gaaatttctg caaggcaaac cgacgagcat ctctatattg
     7921 tggtcgagga tgatggaccc ggtattccat taagcaagcg agaggtcatt ttcgaccgtg
     7981 gtcaacgggt tgatacttta cgccctgggc aaggtgtggg gctggcggta gcccgcgaaa
     8041 tcaccgagca atatgagggt aaaatcgtcg ccggagagag catgcttggc ggtgcgcgga
     8101 tggaggtgat ttttggtcgc cagcattcta cgccgaaaga tgaataaata tgtccatact
     8161 tcacgcatta cgttaagcat ccgttataat cggttgcaga taccagcctg tggatgctta
     8221 acatggaata ccaactcact cttaactggc ccgattttct tgaacgtcac tggcagaaac
     8281 gcccggtggt gttaaaacgc ggctttaata attttattga cccgatctct ccagacgagt
     8341 tggcgggtct ggcgatggaa agcgaagtcg acagtcgact ggtcagtcac caggatggca
     8401 aatggcaggt cagccacggt ccgttcgaaa gctacgatca tctcggtgaa actaactggt
     8461 cattgttagt gcaagcagtg aaccactggc atgagccgac cgccgcgctg atgcgaccgt
     8521 tccgtgaact accggactgg cgtattgatg atctgatgat ctctttttct gtacctggcg
     8581 gcggcgtcgg cccgcatctc gatcagtacg acgtgtttat cattcagggt accggacgtc
     8641 gtcgctggcg agtgggcgaa aaactgcaaa tgaaacagca ctgcccacat ccagatctgt
     8701 tacaggtcga gccgttcgaa gccatcatcg atgaagagct ggagcctggt gatattcttt
     8761 atattccgcc aggattcccg catgaaggct acgctctgga aaatgcgatg aactattccg
     8821 tgggttttcg cgcgccaaat acgcgggaat tgattagtgg atttgccgat tatgtgctgc
     8881 aacgtgaact gggcggcaac tactacagcg atcctgatgt tccacctcgc gctcatcctg
     8941 cggatgttct gccgcaagag atggataaac tgcgtgagat gatgctcgaa ttgattaacc
     9001 agccggaaca ctttaagcaa tggtttgggg agtttatatc tcagtcacgt catgaactgg
     9061 atatcgcgcc gccagagccg ccttatcagc cggatgaaat ctacgatgcg ctgaaacaag
     9121 gtgatgtgct ggtgcgcctg ggtggtctgc gcgtattgcg cattggcgac gacgtgtatg
     9181 ccaatggtga gaagatcgat tccccgcacc gtccggcact ggatgcactc gccagcaaca
     9241 ttgcgctgac tgcggagaat tttggcgatg cgctggaaga tccgtcattc ctcgcgatgc
     9301 tcgcggcgct ggtcaatagc gggtattggt tcttcgaagg gtaagtttgg atttaagccg
     9361 gatgtggcgc tgattgcgcc ttatcgtgcc tacatgcact gaacgcctgt aggcacgata
     9421 agtatgtgga ccagtgtcaa tataacggtt tcatcccggt aattggcagt ccacatacgc
     9481 atcgggcaat tacgacttcc gttgcgccgt taactcggca atacggacga tcacctgcac
     9541 cgctttttcc ataccttcca gagtcacaaa ctcatgctta ccatgatagt tgtaaccgcc
     9601 agtgaacagg ttcgggcacg gtaatcccat aaacgacaac tgcgcgccgt cggtaccacc
     9661 gcggatcggt ttcagttccg gttcaatatc gcagtcacgc atcgcctgct gggcgatatc
     9721 gagaatatgc ggatgctcaa ccactttctc gcgcatattg tagtaactgt cttcaatcgc
     9781 caattcaatg tagcaatcag gatgtaaccc tttgcccact tttttggcga tctccatcat
     9841 tttacgttta cgcgcttcaa actgtttacg gtcgaaatca cggatgatgt agtgcatatc
     9901 ggcccgttca acggtgcctt tcatgctcgc caggtgatag aaaccttcat agccttctgt
     9961 catttccggg ctttcatccg ccggaacttc cgcatgaata cgtgccgcca gcgacagcgc
    10021 atttaccatc actcctttcg ccgtgcccgg atgaacattg ttaccgacaa ttttgatatt
    10081 gaccgatgcg gcgttgaagt tttcaaactc cagttcgcct acgccaccac catcaacagt
    10141 gtaagcccag cgggcatcga aggcatcaac atcaaaatgt ttcgcccctt tgcccacttc
    10201 ttcatccggg gtaaaggcga cgcgaatatc accatgcgga atgtttttct gttgcaatac
    10261 cgccagcgcg gtcatgattt ctgcaatacc tgctttgtca tcggcaccta acaaggtttt
    10321 accatcggtg gtaatcagcg tctgacccag tagctgatgc agcaccggga acataaccgg
    10381 tgataaaact tcatcgccga tacccagcgc aatatcgcca ccgcgatagt tttcaacaat
    10441 ttgcggattc acatttttgc cgctgcaatc cggtgaggta tccacatgag aaataaagcc
    10501 aatcgccggg atatcgccag ggacgttagc cggtaacgtc gccatcaaag tgcccttctc
    10561 acttaaggtc acattgataa gccccatctc ttcgagctgc tctttcagca gatgcaataa
    10621 cttccattgg ccttccgtgc tgggaacctg tctcacccct gcttttgatt gggtatccag
    10681 agacacgtag ttcaaaaatc gctcaagtag tttatccatg tagtcaccct cactttttgt
    10741 gacaacatta ttaagaagca agaaaagaca aatattgcgt caggtcactt ttacccctgc
    10801 aagcgggaat atttatcagc attaccttaa tgaatataaa gctaagccag taattcacaa
    10861 caaagattgg cgattcaagc ggtttgccgt agaattaccg ccctcattaa gagtcaccaa
    10921 ggtggttaac cacaaacccc gcatcggtaa gccatccgtt gcgtttacat gggacagagt
    10981 aaaaaattga ataaacaacc gagttcgctt tcaccgctgg tgcaattggc gggaattcgc
    11041 aaatgctttg atggtaaaga ggtcattccc cagctggatc tgactatcaa caatggcgag
    11101 ttcctcacgc tgcttggccc ttctggctgc ggtaaaacaa ccgttcttcg cctgattgca
    11161 ggtctggaaa ctgttgattc cggacgcatc atgttggata acgaggacat cacccacgtt
    11221 ccggcggaaa accgctatgt gaacactgtt ttccaaagct acgcactttt cccccacatg
    11281 accgtgttcg aaaatgtggc ctttgggttg cgcatgcaaa aaacccccgc tgctgaaatt
    11341 acgccccgcg tgatggaagc cctgcggatg gtgcagctgg aaaccttcgc tcaacgcaaa
    11401 ccgcatcagc tctctggtgg tcaacagcaa cgcgtcgcca ttgctcgcgc ggtggttaac
    11461 aagcctcgtc tgttgttgct ggatgagtcg ctctcagcgc tggattacaa actgcgtaag
    11521 caaatgcaga acgagctgaa agcgttacag cgtaagcttg gcattacatt cgtcttcgtg
    11581 actcacgatc aggaagaagc actcaccatg tcagacagga ttgtggtgat gcgcgatggt
    11641 cgcattgagc aagacggcac gccgcgtgaa atctacgaag agccgaaaaa cctgtttgtt
    11701 gccgggttca ttggcgaaat caatatgttt aacgccactg tcatcgaacg tctcgacgag
    11761 cagcgcgtac gcgccaacgt tgaaggccgc gaatgtaata tctacgttaa cttcgccgtt
    11821 gaaccggggc aaaaactgca tgttctgctg cgcccggaag acttacgtgt tgaagagatt
    11881 aacgacgaca accacgctga agggctgatt ggttacgttc gcgagcgtaa ctacaaaggc
    11941 atgacgctgg agtcggttgt tgaactggaa aatggcaaga tggtgatggt cagcgaattc
    12001 tttaatgaag acgatcctga ctttgaccac tctctcgacc aaaaaatggc cattaattgg
    12061 gtagaaagct gggaggtcgt actggctgat gaagaacaca agtaagttcc agaatgtagt
    12121 gattgtcact attgtcggtt ggcttgtgtt gtttgtcttt ctgcccaacc tgatgatcat
    12181 tggcaccagc tttttgaccc gcgacgacgc cagtttcgtc aaaatggtct ttacgctgga
    12241 taactacacg cgtctgctcg atccgctcta ttttgaagtg ctattgcact cgctgaatat
    12301 ggcgctgatc gccactctcg cctgcctggt gctgggctac ccgtttgcct ggtttctggc
    12361 gaagctgcca cacaaggtgc gtccgctgct gctgtttctg ctgattgttc ccttctggac
    12421 caactcactg attcgcatct acgggctgaa aattttcctc agcaccaaag gctatctcaa
    12481 cgagtttttg ctctggctgg gcgttatcga cacaccaatc cgtatcatgt tcacgcccag
    12541 tgcggtgatt atcggtctgg tttacattct gctaccgttt atggtgatgc cgctgtactc
    12601 cagtatcgaa aaactggata agccgttgct tgaggcggcg cgcgatctcg gtgccagcaa
    12661 gttacagact tttatccgca tcattattcc actgacgatg ccggggatta ttgccggatg
    12721 tctgctggtg atgctgccag cgatgggcct gttctatgtg tccgacctga tgggcggtgc
    12781 gaaaaacctg ctgatcggta acgtcatcaa ggtacagttc ctcaatattc gtgactggcc
    12841 gtttggtgca gccaccagca ttacgctgac tatcgtaatg ggcctgatgt tgctggttta
    12901 ctggcgcgct tctcgtctgc tgaataaaaa ggtggaactc gaatgatcgg tcgactgctt
    12961 cgcggcggtt ttatgaccgc tatctacgcg tacctgtata tcccaatcat tattttgatt
    13021 gtgaactcct ttaacagctc gcgctttggc atcaactggc agggttttac cactaaatgg
    13081 tatagcttgc tgatgaacaa cgacagcctg ttacaggcag cgcagcattc actaacaatg
    13141 gcggtgtttt cggcgacgtt tgctacgctt atcggttcac tgacggcagt tgcactgtac
    13201 cgttatcgct ttcgtggtaa gccgttcgtt agcggaatgc tgtttgtggt aatgatgtca
    13261 ccagatatcg tgatggcaat ttcgctgctg gtgctgttta tgctgctggg tattcagctt
    13321 ggcttctggt cgctgctgtt ctcgcatatc accttctgcc tgccgtttgt ggtggtgacg
    13381 gtatattcgc gcctgaaagg ttttgacgtg cggatgctgg aagcggcgaa agatctcggt
    13441 gccagtgaat ttaccattct gcgaaaaatc attctgccac tggcaatgcc tgcggtggcg
    13501 gcaggctggg tgttaagctt taccctgtcg atggacgatg tggtagtttc ttcgttcgtc
    13561 accggaccaa gctatgaaat tctgccgtta aaaatttatt cgatggtcaa agtcggcgta
    13621 tcgccggaag ttaacgcgct ggcaaccata ttactggtgc tgtcgctggt gatggttatt
    13681 gccagccagc ttattgctcg tgataaaacg aaaggtaaca caggggacgt taaatgaaaa
    13741 aatggtcacg ccacctgctc gcggcgggtg ctctggcact gggcatgagc gccgctcacg
    13801 ccgatgacaa caatacgctg tatttctaca actggaccga gtacgtgccg ccaggactgc
    13861 ttgaacagtt caccaaagaa accggtatta aggttatcta ttcgacttac gagtcgaacg
    13921 aaaccatgta cgccaagctg aaaacgtaca aagacggtgc ctatgacctg gtggttcctt
    13981 caacctatta cgtcgataaa atgcgtaaag aagggatgat ccagaagatc gacaagtcga
    14041 agttaacgaa cttcagcaat ctcgatccag acatgctcaa taagccgttt gacccgaata
    14101 acgattactc cattccgtat atctggggtg cgacggcgat tggcgttaac ggtgatgcgg
    14161 tggatccgaa atctgtcacc agctgggccg atttgtggaa gcctgagtac aaaggcagcc
    14221 tgctgctgac cgacgatgcc cgtgaagtgt tccagatggc gctacgtaag ctgggctact
    14281 ccggtaacac caccgatccg aaagagattg aagctgcata taacgagctg aaaaaactga
    14341 tgccaaacgt cgcagcgttt aactccgata acccggctaa cccgtacatg gaaggcgaag
    14401 ttaacctcgg catgatctgg aacggttctg ctttcgttgc acgccaggcg ggtacgccta
    14461 ttgacgtggt gtggccgaaa gaaggcggca tattctggat ggacagcctg gcgatcccgg
    14521 caaatgccaa aaacaaagaa ggcgcgctga aattgatcaa cttcctgctg cgcccggatg
    14581 tggcaaaaca ggttgctgaa actatcggtt atccaacgcc aaaccttgcg gcgcgtaagc
    14641 tgttaagtcc agaagtggcg aacgataaga cgctctaccc ggatgctgaa accattaaaa
    14701 atggcgaatg gcagaatgac gttggcgcag ccagcagcat ttatgaagag tattatcaga
    14761 agctgaaagc aggacgttaa ttcagcaagc gggcggtggt attaccgccc gttttttagt
    14821 tagcaaggat gtgactaatg agtcaggaca gtaaagtttt ctttcgtata ttcttcggta
    14881 tcggtctggt actgatcctc atttctgttg ttatctttta taaccagttc acctatagca
    14941 aagacgcgat tcataccgaa ggcgtcattg ttgataccgt cttgcactcc agtcattctc
    15001 atcgcacggg taaaaatggt tcgtggtatc ccgtcgttgc ctttcgaccg acgccagatt
    15061 acacgctaat tttcaattcg agtattggca gcgattttta tgaagacagc gaaggcgata
    15121 aagttaatgt ttattattct cccgggcatc ctgaaaaggc tgagataaat aacccgtggg
    15181 ttaatttttt taaatggggg tttatcggca ttatgggcgt tatttttatc gccgtcggtt
    15241 tattaatcag tatgccctcc tctaaaaaat cacgcaggaa acggaagtcc cgtccatgaa
    15301 aaaaatcata atactgctca gtttactgat acttctgcct ctgacggtaa ccagtaagcc
    15361 cttaatccca ataatgaaga cactttttac ggatgttaca ggtactgtac cggatgctga
    15421 ggagattgcg cggaaagcgg agctttttcg tcaacaaaca gggattgcgc cgtttatcgt
    15481 ggttgtacct ggcgttaatg aagcaagctt caggcaaaat ggcaaagcga tgctggcgca
    15541 tgcgtcatct tcattgagtg atgtaaaagg aagtgttctg ttacttttta ctacacgcga
    15601 gccacggtta attatgataa ccaacggcca tgttgaaagc agcatggacg ataagcatct
    15661 cggccttctg gtagaaaatc acacgctggc ttatttacat gcggatctct ggtatcaggg
    15721 aatcaataat gcattggctg ttttacaagc acagatatta aaacaaccga cgccgccact
    15781 gacatattat ccgcatcccg gacaacagca ggagaatgat cctcccggca gcaccacgac
    15841 attaggcctt tttgcctggg cggttgcatt tatcgtcttt actgcatttt ttaactacac
    15901 gacgcgcctt aattatgcat taaaatttgc agtggcgatg actattgcca atatgggcta
    15961 tcaggcttta tgcctgtata tcgataatag ctttgcgatt accagaatat cgccactctg
    16021 ggcagggctg attggtgtct gtacgtttat tgccgcgttg ttattgacga gtaaacggta
    16081 aaagagatag taaaatgcgt gtgtattatg ccggatgcga cgcgaacgcc ttatccggcc
    16141 cacaaccccg caaattcaat taattgcgtc cccttgtagg cctgataagc gtagcgcatc
    16201 aggcaatgct tcctgcttta taatcccttc agcaactttt caacaaattc tggcaccacc
    16261 tggcttgccg ggccgtaata tttctcggca aattcattac caacctgact cggttcaaga
    16321 ttcagttcca cggtgtgcgc gccatgcagt ttcgcttcgt gaacaaaccc agccgccgga
    16381 taaacatgcc ccgaagtacc aatggcaatg aaaatatcgg ccatcgacaa cgccatataa
    16441 atttcatcca tgccgagtgg catttcgcca aaccacacta cgtgcgggcg caagggtgcc
    16501 gggaactggc agcaatggca tttatcttct ggggtaacgt ctccgatcca gtcgagaacc
    16561 tgaccacttt gtgaacaacg aactttcagc agttccccat gcatatgaat cacattggta
    16621 ttacctgcgc gttcatgcag gttgtctata ttctgcgtca ccagcaaaaa gcgatcgccg
    16681 agagcatctt gcagtttagc cagcgcaaga tgcgcggcgt taggctgaat ttctggctgc
    16741 tgcagctgtc gacgacgggc gttataaaac gcttgcacca gttcaggatc gcgatcgaaa
    16801 ccttccggcg ttgccacatc ttcaacccga tgttcttccc acaggccatc tgcggcgcga
    16861 aaggtacgaa tacctgattc cgcagaaatt cctgcccctg tcagtacgag tactcttggt
    16921 ttttccattg cttccggcac cactttatct ctgaaaaaaa tacgctgacg caaacgctcg
    16981 cgcaggcggc gtttattttt acgaaaacga cttaaccgat gaccccgacg cgacagcata
    17041 gcaacctctg ttgtttaatc ggttagatgt aggaaggccg caccacgcat tcctcccgca
    17101 tcaccgtggc gcgcacgttc aatgcgcgga acacgagcta caggtaagag atgacgaggc
    17161 agcctgtccg ccagttgcgt tgtgattgcc gggaaattcg ataagccacc accaatgacg
    17221 accaggtcag ggtcaacaat ggtcaggata tttcccagac aaaccgctaa taaatccaga
    17281 taacgctcaa cgtgcgccct tgcctgctca tcgccttgat cataaagcgc aataatttcg
    17341 ggagcctgca acggttgatg ataatagtgt tgatacagcc acgcaaaacc gcgaccagac
    17401 agataatttt caatgcagcc atgctgacca cagccgcagc ggcgtaacgg gaaatccaac
    17461 cccatcatgg ttaacgcatc aaccggcaga cgcatatggc caaactcgcc ggtaatgtag
    17521 cttttaccgg taatcggttt gccgttgaaa atcagcccgc cgccaacgcc ggtgccgaga
    17581 atcaacccca tcaccagtgg atattgcgta aattcatcat cccaggcttc tgaaagggca
    17641 aaacagttgg catcgttatc aaggcgtaca tcgcgatcaa gacgtgcgct caggtcggca
    17701 cgcagcggtt taccgctggc agcggggaca ttggcggcat acagcgtacc atcttctgtt
    17761 tccggcattc ccggaatacc gatgccgaca gaacctttac agccaaaacg ttggtcagct
    17821 tcagctacca gctcacacac tgcatctaaa aatgcgtcat agctgtcacg cggtgtcggc
    17881 acccgctttt ccaactgcaa ctgccgaccg ctatcaaaca cgccaagcgc aatttttgtt
    17941 ccaccaatat caaacccgta atacattgcc gctccttgtt ttaatgtact gcctttactg
    18001 gccgctaagg actcgcgcag ggtcaatatt actggcgcgc cgcgccggat accaacttgc
    18061 caaaagactc agcaacaatg ctgtgaccag tacgtagaag acgtccagcc agtgcaattc
    18121 cgatggcagg aaatcaataa aatagatatc gctggagagg aactgatgac cgatcaactt
    18181 ttcaatccac tcaataatcg gggtaagttg cagtgaaacc actacgccga taatcacacc
    18241 acacaggctg ccgaacagcc ctgccagcaa tccataccag acaaagatgg cgcgaattaa
    18301 accatctttc gcccccagcg ttcttaatac tgcgatatcg ccactcttgt ctttcaccgc
    18361 catcactaag gtggagacga tgttgaaaca ggccacgcca atcaccagta ccatcgccag
    18421 atacataatg gcgcggatca tttggatatc gcgatacata tagccgtaag taccaatcca
    18481 gcttttaata taaacatagc tgttggtcac ttcacccgca tcgcgtacca gcttattggc
    18541 gttgaaaaca tccgtcattt taagggcaat acctgacacg ctggaaccca tatcaagata
    18601 ttgttgggca tccgccagcg ggatcatggc aaaactgtga tcgagttgac cactcaactg
    18661 caaaataccg gcaacgtgca aacgcacacg ttttggctgc atcagtttat gctcgggatt
    18721 cgagttgggg atcataatcg acacccaatc cccctgcttc actttcagcg catccgccac
    18781 gcctttgccg atgataattt gctgttcgcc cgctttaaaa ttgcgccacg catcgccctg
    18841 gacaaacgag ggtaatgcgc tcagacgctg ttcctgttgc gggttaacgc ccttcacctg
    18901 gattgcgcga agattcgcgc cactttccac cagcccggtg aaattgatat acggcgcggc
    18961 agcggcaata cccggcactt tttgcacgtg atccagtgct tcctgccagt tagtccacgg
    19021 ctgatccacc gcctcgattt caccatgcgg caccaccgcc agaatacggt tgttcagttc
    19081 gcgttcaaag ccgttcatcg cgcttaagcc gacgatcaat accgccacgc cgagggcaat
    19141 gccaatggta gaaatgacgg agatcagcga caccatgccg ccgcgccgcc gtccgcgact
    19201 aaaacgcagg ccaattaata acgataaagg catcgccatt actccgcccc catcaggctc
    19261 agttccgccg tcagacgacc atcacgcatt tctaactggc ggctcatacg tttcgccagt
    19321 tgcaggtcgt gagtaaccac caggaaggcg gtgccctgca agcgattcaa ttccccaagc
    19381 aactggaaga tgctgtcggc gttacgcgca tcgaggttac cggtaggttc atccgccagt
    19441 accaggcgcg ggttattcac cagcgcacgg gcaatcgcca cacgctggcg ttcgccgcca
    19501 gaaagttcag atgggcggtg attcgcacga tgatccagcc ccaccgcttt taacatctca
    19561 agtgcacggc tgttgatttc agcgggcttt ttcttgccaa tcagcagcgg catagccacg
    19621 ttttccaggg cggtaaaatc cggcagcagg tggtgaaact gataaataaa gcccagcttc
    19681 tggttgcgca gctctgcttt cgccgccgaa gacagtttgc tcatcggttg accgttaaag
    19741 atcacatcgc cggaggttgg cgtatccagc ccgcccaaca agtgcagcaa ggtgctttta
    19801 ccggaaccag agctaccgac gatcgccatc atttcacctt cgccgacgct gaaactgaca
    19861 ttgtgcagca catcggtttg cacactgcct tcctgatagc gtttgcacag gttgtcgcat
    19921 tgcaacagga tcttattcat aacgtaaagc ctcagcgggt tgagtggcgg cagcgcgcca
    19981 tgaagggtaa agcgtagaca gcagcgcgat agccatcgcc accagcgcaa taacaatgac
    20041 ctgtaaaggt tcgatagcga ccggcagcgc cgcgccatca agcaggacgc cgattatcgg
    20101 catcagatta tttaactggc tggcaagcag ggcgccaagc gccgctccga ggatcgcacc
    20161 gataatcccg gcgctggccc cttgcaccat aaagaccatc atgatttgtc gcggagttaa
    20221 gccttgcgtt tgcaggatcg ctacttcgcc ctgcttctcc attaccatca gccctagtga
    20281 ggtaataatg ttaaacgccg caacggcgac aatcaggctc agcagtaaac ccatcatatt
    20341 tttttccatg cgtacggcct ggaacaactc gcctttacgg tcacgccagt cctgccattt
    20401 gctgccttca ggcagttttt gctgacttaa agagtcaact ttcagcggct catccagcca
    20461 caaacgccag ccggtaatat tgcctgccgg ataacgcatt agacgcgagg catcctcaat
    20521 attcaccagc atttcatagc catcaacttc actgttagcg gcaaaagtac caatcacatt
    20581 gaacaggcgc tggcttggaa tacgccccat cggcgtgaac tggctggcag atggcaccat
    20641 cacgcggatt tgatcaccgc gattgacgcc caactgtgag gcaagctgtt caccgaggat
    20701 gacattatat ttccccggct cgaggtcagt ttgtttcaca ttgaccagat acggcgttag
    20761 tggatctttt tgcgccggat cgatacccag catcaccccg accgccacgc tgcgcgcgct
    20821 ttgcagtacc acatcaccgg tagtaatagg tgcgacgcga ttaacgccgt ccagtttgac
    20881 tgccgtttcc gggagttgct gcgggttaag agagccatgc tcagaagaga gaattgcctg
    20941 tggcatcagg ccaaggatgt tgttttgcag ctcgcgctca aagccgttca tcactgacaa
    21001 tactgtgacc agcgccatca ccccgagggt aatgccgatg gtagaaagcc aggagacgaa
    21061 acgaccgaag cgatccgctg cacgcccacg catgtaacgc aggccaataa atagagcgac
    21121 aggttggtac atgaaatccg tctggttgct gtagcaaaga cacgagtata taagcgaaag
    21181 caagatgggt aaacgtctga actgtaagtg atcgtcaggg ctaatctgaa aagcccccgt
    21241 aaatcaatcg gctaaattgc catgttttat catcaatctt gccgcctgat cttaatctga
    21301 aaaatcatcc aatacagaca gatacccatt acacctcggg tcataacccg caggatcaac
    21361 cgcatgtaaa ttaaaaaaga gcgtgttgtc ctgatgaaac aaaaagagct atggattaac
    21421 cagatcaaag ggttatgtat ttgtctggtc gtgatttatc actcggtcat taccttttat
    21481 ccgcatatga ccacttttca gcatccgtta tcggaagtcc tgagcaaatg ctggatctat
    21541 ttcaatcttt accttgcacc ctttcgtatg ccggtttttt tctttatatc cggctatttg
    21601 attcgccgct atatcgacag cgtaccgtgg ggaaattgtc tcgataaacg gatctggaac
    21661 atcttctggg tgctggcgct ttggggcgtg gtgcagtggc tggcgttaag tgcgctgaac
    21721 cagtggctgg cacctgagcg cgatttaagc aacgcttcca atgccgcgta tgccgattcc
    21781 accggtgagt tcctgcacgg gatgctcacc gccagcacca gcttgtggta tctgtatgcg
    21841 ttaattgtct atttcgtggt atgtaaaatt tttagccgtc tggcgctgcc actattcgcc
    21901 ttgtttgtac tgctgagtgt ggcggttaat ttcgttccca cgccgtggtg gggaatgaac
    21961 agtgtgatcc gcaatttgcc ttattacagc cttggcgcat ggtttggcgc aacaataatg
    22021 acctgtgtta aagaggtgcc gttgcgccgc catctgctga tggcttcttt gcttaccgtt
    22081 ctggcggtcg gtgcctggtt gtttactatc tcgctgctgt tgtcgctggt atcgattgtg
    22141 gtgatcatga agctgtttta tcaatacgag caacgattcg gcatgcgctc caccagcctg
    22201 ctgaatgtga ttggctccaa caccattgct atctacacca cccatcgcat tctggttgaa
    22261 atattcagct taactctgct tgcgcaaatg aacgcagcac gctggtcgcc gcaagtcgaa
    22321 ctgacactcc tgctggttta tccctttgtc agtttgttca tctgtactgt tgcgggcttg
    22381 ctggtaagaa aactttcaca gcgtgcattc agcgatctgt tgttctcccc gccttcactg
    22441 cccgctgccg tcagttactc ccgctaagcc atcgccccag ccggggcgat tcaatttgct
    22501 aaaccatgtc gattacggat aatgaacagc attgcttatc agagatatgc ccccatatgt
    22561 tgaggcatat cctaacgaga atctgacaac cgttatgcct gaacaatatc gttatacgct
    22621 gcccgtcaaa gcgggtgagc agcgtctgct gggcgagtta accggcgctg cctgcgcgac
    22681 gctggtggcg gaaattgccg aacgtcacgc cggtccggtg gtactcattg caccagatat
    22741 gcaaaatgct ctgcgtttgc atgatgaaat cagccagttc accgatcaga tggtgatgaa
    22801 tctggcggac tgggaaaccc tcccttacga cagtttttcg cctcatcagg acattatctc
    22861 ctcgcgcctt tccacccttt accagctacc gacgatgcag cgtggcgtac tgattgttcc
    22921 ggtgaatacg cttatgcagc gcgtttgccc acacagtttt ctccacggtc atgcgctggt
    22981 gatgaaaaaa ggtcagcgcc tgtcacgaga tgcattacga acccaactgg acagcgccgg
    23041 ttatcgccat gttgaccagg tgatggagca cggcgaatac gccacgcgcg gcgcgttgct
    23101 ggatctcttc ccgatgggga gtgagctgcc ttatcgtctt gatttctttg atgatgaaat
    23161 cgacagcctg cgggtgtttg acgtcgacag ccagcgcact ctggaggaag tagaagcgat
    23221 caatctgctg cccgcgcacg aatttccgac cgataaagcg gcaattgaac tgttccgcag
    23281 ccagtggcgc gataccttcg aagtgaagcg cgatccggaa catatttacc agcaagtgag
    23341 taaaggtaca ttgcctgccg ggatcgagta ctggcagccg ctattcttca gcgaaccgct
    23401 gccgccactg ttcagttatt tccctgccaa taccttgctg gtgaatactg gcgatctgga
    23461 aaacagtgcc gaacgtttcc aggctgatac actggcgcgt tttgagaatc gcggcgtcga
    23521 tccgatgcgc ccactgttgc caccacaatc gctctggctg cgggtggacg agctcttctc
    23581 agagctgaaa cactggcccc gagtgcagct aaaaactgaa catttaccga caaaagccgc
    23641 gaatgccaat ttaggtttcc agaaactgcc agacctggcc gttcaggcac aacaaaaagc
    23701 gccgctggat gcgctgcgta agttcctcga gactttcgac ggtccggtgg tgttctcggt
    23761 agaaagtgaa ggtcgccgtg aagcgctggg tgaactgctc gcacgaatta aaattgctcc
    23821 gcaacgcatt atgcgtcttg atgaagccag cgaccgtggg cgttatctga tgattggcgc
    23881 tgccgaacat ggttttgtcg ataaggtgcg taacctggcg ctgatttgcg aaagcgatct
    23941 gctcggtgaa cgcgttgccc gccgtcgtca ggactctcgc cgcaccatca accccgatac
    24001 actgatccgc aacctcgcgg agctgcatat tggtcagccg gtagtccatc tggagcacgg
    24061 cgtcggtcgt tatgccggaa tgaccacgct ggaagctggc ggcattactg gcgagtacct
    24121 gatgctcacc tatgccaacg acgccaaact gtatgttccg gtgtcgtcac tgcatctgat
    24181 tagccgttac gcaggtggcg cggaagaaaa cgccccgctg cataaacttg gcggtgatgc
    24241 gtggtcacgc gcgcggcaga aagcggcgga aaaagtgcgt gatgtggcgg cggaattgct
    24301 ggatatctac gcgcaacgcg ccgccaaaga gggcttcgcg tttaaacacg atcgtgagca
    24361 gtatcagttg ttctgcgaca gcttcccgtt tgaaaccacg ccggatcagg cgcaggccat
    24421 taatgcggtg cttagcgaca tgtgtcagcc actggcaatg gatcgtctgg tgtgcggcga
    24481 tgttggcttt ggtaaaacag aagtggcgat gcgcgccgct ttcctggcag tagataacca
    24541 caagcaggtg gcggtgctgg tgcctaccac ccttctcgcg cagcagcatt acgacaactt
    24601 ccgcgaccgt ttcgccaact ggccggtacg tatcgaaatg atctcccgtt tccgcagcgc
    24661 caaagagcag acgcaaatcc ttgcggaagt ggcggaaggg aaaatcgata ttctgatcgg
    24721 tacgcacaaa ctgctgcaaa gtgacgtcaa gtttaaagat ttaggcctgc tgattgtcga
    24781 tgaagaacac cgcttcgggg tgcgtcataa agagcgcatt aaagcgatgc gcgcgaacgt
    24841 ggatattctg acgcttactg caacgccgat cccacgtacg ctgaatatgg caatgagcgg
    24901 aatgcgtgac ctgtcgatta tcgccacgcc gcccgcccgt cgtctggcag ttaaaacctt
    24961 tgtccgtgag tatgacagcc tggtggtccg ggaggcgatc ctgcgtgaaa ttttgcgcgg
    25021 aggacaggtt tattatctct acaatgatgt ggaaaacatt cagaaagccg ccgaacggct
    25081 ggcagaactg gtgccagaag cgcggatcgc catcggtcac gggcagatgc gcgagcgcga
    25141 actggaacgg gtgatgaatg atttccatca tcaacgtttc aacgtgctgg tttgtacaac
    25201 cattatcgaa accgggatcg acatcccgac agccaacacc attatcattg aacgcgcgga
    25261 tcacttcggt ctggcgcagc tgcaccagtt acgcggtcgc gtcggacgtt cgcatcatca
    25321 ggcatatgca tggttgctga caccgcatcc aaaagcgatg actaccgatg cgcaaaaacg
    25381 tcttgaagcg attgcctcgc tggaagatct cggggcaggt tttgcgctgg caacgcacga
    25441 tctggagatt cgcggcgcgg gtgaactgct tggcgaagaa caaagcggct caatggaaac
    25501 catcggtttc tcgctgtata tggagttgct ggaaaacgcc gtcgatgcac tgaaagccgg
    25561 acgcgagccg tcgctggaag atctcaccag ccagcaaaca gaagtcgagc tgcggatgcc
    25621 gtcgctattg ccagatgatt tcattcctga cgtgaatacg cgtctgtcgt tctacaaacg
    25681 tattgccagc gccaaaacgg aaaacgaact ggaagagatc aaagtcgagc ttatcgatcg
    25741 cttcggcctg ctgccggatc cggcgcgtac cctgctggat attgcccgtc tgcgccagca
    25801 agcgcagaaa ctggggatca ggaagctgga aggtaatgag aaaggcgggg tgatcgaatt
    25861 tgccgagaag aatcacgtta atccggcctg gttgattggt ttgctgcaaa aacagccgca
    25921 gcattaccgc cttgatggtc cgacgcgcct gaaatttatt caggatttga gtgagcggaa
    25981 aacacgtatc gaatgggtac gccagtttat gcgtgaactg gaagagaacg cgatcgctta
    26041 atggtaaatc gggggcgttt ctgcgccccc atctgcaaca tttacaaatt ctttgcactt
    26101 ccctgcacta tccgacactg tcaccatcca taattcaggc ttatctgttt attacaataa
    26161 ccttatattt attatggatt tttggtgatg atcaaaacgc gtttttctcg ctggctaacg
    26221 ttttttacgt tcgccgctgc cgtggcgctg gcgctaccgg caaaagccaa cacctggccg
    26281 ctgccgccag cgggcagtcg tctggttggc gaaaacaaat ttcatgtggt ggaaaatgac
    26341 ggtggttctc tggaagccat cgccaaaaaa tataacgtcg gctttctcgc tctgttacag
    26401 gctaaccccg gcgttgatcc ttacgtaccg cgcgcgggca gcgtgttaac gatcccgttg
    26461 caaaccctac ttccagacgc gccgcgcgaa ggcattgtga tcaacattgc ggagctgcgt
    26521 ctctattact acccgccggg taaaaattcg gtaaccgtgt atccaatagg tattggtcag
    26581 ttaggtggtg acacgctgac accgacaatg gtgaccaccg tttcagacaa acgtgcaaac
    26641 ccaacctgga cgccaacggc aaacatccgc gcccgttata aagcacaggg aattgagttg
    26701 cctgcggtag tgccggctgg accggataac ccaatgggcc atcatgcgat tcgtctggcg
    26761 gcctatggcg gcgtttattt gcttcatggt acgaacgccg atttcggcat tggcatgcgg
    26821 gtaagttctg gctgtattcg tctgcgggat gacgatatca aaacactctt tagccaggtc
    26881 accccaggca ccaaagtgaa tatcatcaac actccgataa aagtctctgc cgaaccaaac
    26941 ggtgcgcgtc tggttgaagt acatcagccg ctgtcagaga agattgatga cgatccgcag
    27001 ctgctgccaa ttacgctgaa tagcgcaatg caatcattta aagatgcagc acaaactgac
    27061 gctgaagtga tgcaacatgt gatggatgtc cgttccggga tgccggtgga tgtccgccgt
    27121 catcaagtga gcccacaaac tctgtaaatc ctggataaaa aaaatccccg cgacacggca
    27181 tgtcgcgggg aaaaaatcag cagtagcagg cattaatgag ggttaatgct tatttataaa
    27241 ttactgctgt tccatggagg gtattcggac cggttacaga agtaatacgg aaagattttg
    27301 cgcccatctc atccgctttt tgcgccagct gctcttccag cgatcccaga tttgtccccg
    27361 cgttagcact gattgtaccg actttttgtt ggccttctgg cgttgactga acttcgacag
    27421 ccgcaaagct ggcaaatgac atggagctta aaatcgccgc agcgatgagg gtttttacgt
    27481 ttttcataat agtggcctta tgcagatgaa tgacgtcgca agcaattact taacgatcga
    27541 taacta
//
LOCUS       DAPGOF010000055        26814 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.055, whole genome shotgun sequence.
ACCESSION   DAPGOF010000055 DAPGOF010000000
VERSION     DAPGOF010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.055"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            346..561
                     /gene="essD"
                     /locus_tag="RHV13_002002"
     CDS             346..561
                     /gene="essD"
                     /locus_tag="RHV13_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HDV1241535.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            561..1058
                     /gene="rrrD"
                     /locus_tag="RHV13_002003"
     CDS             561..1058
                     /gene="rrrD"
                     /locus_tag="RHV13_002003"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HDV1241536.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMLGKTYTKAECKALLNKDLATVARQINPYIKVDIPETMRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREIC
                     LWGQQ"
     gene            1275..1457
                     /gene="rzoD"
                     /locus_tag="RHV13_002004"
     CDS             1275..1457
                     /gene="rzoD"
                     /locus_tag="RHV13_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HDV1241537.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(1548..1841)
                     /gene="iss"
                     /locus_tag="RHV13_002005"
     CDS             complement(1548..1841)
                     /gene="iss"
                     /locus_tag="RHV13_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HDV1241538.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQEKTVDAAKICGGAENVVKTETQQTFVNGFLGFITLGIYTPLEARVYCSQ"
     gene            complement(2201..2395)
                     /locus_tag="RHV13_002006"
     CDS             complement(2201..2395)
                     /locus_tag="RHV13_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HDV1241539.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIQSDDG"
     gene            2785..3330
                     /locus_tag="RHV13_002007"
     CDS             2785..3330
                     /locus_tag="RHV13_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HDV1241540.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            3305..5230
                     /locus_tag="RHV13_002008"
     CDS             3305..5230
                     /locus_tag="RHV13_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HDV1241541.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            5227..5433
                     /locus_tag="RHV13_002009"
     CDS             5227..5433
                     /locus_tag="RHV13_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family protein"
                     /protein_id="HDV1241542.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            5430..7031
                     /locus_tag="RHV13_002010"
     CDS             5430..7031
                     /locus_tag="RHV13_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDV1241543.1"
                     /translation="MKTSSIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPSES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAACKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            7012..8331
                     /locus_tag="RHV13_002011"
     CDS             7012..8331
                     /locus_tag="RHV13_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HDV1241544.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPGDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTDVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            8341..8673
                     /locus_tag="RHV13_002012"
     CDS             8341..8673
                     /locus_tag="RHV13_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HDV1241545.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGTAVGILAVDADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            8729..9754
                     /locus_tag="RHV13_002013"
     CDS             8729..9754
                     /locus_tag="RHV13_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HDV1241546.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSHSELETAVKDLGEAVSYKGMYGDTAIVVYSGQYVENDVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDVDAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDA
                     FVSVQLA"
     gene            9796..10191
                     /locus_tag="RHV13_002014"
     CDS             9796..10191
                     /locus_tag="RHV13_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HDV1241547.1"
                     /translation="MTKDELIARLQVLGEQLNRDVSLTGTKEELVLRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQPDTVIDTAALVTVVALVTLHTDALHATRDEP
                     VAFVLPGTAFRVSAGVAAEMTEHGLARMQ"
     gene            10203..10556
                     /locus_tag="RHV13_002015"
     CDS             10203..10556
                     /locus_tag="RHV13_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HDV1241548.1"
                     /translation="MADSDNLFDAAIARADETIRGYMGTSATMTSGELSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWVDRISPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            10568..11146
                     /locus_tag="RHV13_002016"
     CDS             10568..11146
                     /locus_tag="RHV13_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV1241549.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIRVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            11143..11538
                     /locus_tag="RHV13_002017"
     CDS             11143..11538
                     /locus_tag="RHV13_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV1241550.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFNGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            11546..12286
                     /locus_tag="RHV13_002018"
     CDS             11546..12286
                     /locus_tag="RHV13_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV1241551.1"
                     /translation="MPVPNPVMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGELAAVAEITVTAS"
     gene            12302..12724
                     /locus_tag="RHV13_002019"
     CDS             12302..12724
                     /locus_tag="RHV13_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HDV1241552.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFLVAMSLWHNHPQKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNNAPEQTEDAGPAEPVSAGKCSTVS"
     gene            12706..13140
                     /locus_tag="RHV13_002020"
     CDS             12706..13140
                     /locus_tag="RHV13_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HDV1241553.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            13133..15712
                     /locus_tag="RHV13_002021"
     CDS             13133..15712
                     /locus_tag="RHV13_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDV1241554.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTETDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLTALVNAGVRGGEQFEAISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDSVLDIGRPDTAQGMLEKAEKAFDE
                     ADKKWQWYQSRSHRRGKTSAFLANLRGAWEDRANAQLGLSAATLQADLEKAREMAAKD
                     WAESEASRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAK
                     KDYEATLKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDL
                     WKAESQFAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQERLNALAQ
                     QADKFAQQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNPLALNNVMSEQKKT
                     WAAEDQLRRSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQN
                     WRSFTRSVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFA
                     TGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMAD
                     SRSQASGTFEQNNHVVINNDGTNGQIGPAALKAVYDMARKGARDEIQTQMRDGGLFSG
                     GGR"
     gene            15709..16038
                     /locus_tag="RHV13_002022"
     CDS             15709..16038
                     /locus_tag="RHV13_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV1241555.1"
                     /translation="MKTFRWKVKPGMDVASVPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEATVLESFLEEHGGWKSFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            16038..16736
                     /locus_tag="RHV13_002023"
     CDS             16038..16736
                     /locus_tag="RHV13_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDV1241556.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDMQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            16742..17485
                     /locus_tag="RHV13_002024"
     CDS             16742..17485
                     /locus_tag="RHV13_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV1241557.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLRAEMQGEIVALVHSHPGGLPWLSETDRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLSGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDSELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            17422..18054
                     /locus_tag="RHV13_002025"
     CDS             17422..18054
                     /locus_tag="RHV13_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HDV1241558.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDVSTSGLTAQLHETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGS
                     FFTAGATLAAWGAAIGAGGMTGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQN
                     TYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            18114..21611
                     /locus_tag="RHV13_002026"
     CDS             18114..21611
                     /locus_tag="RHV13_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HDV1241559.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITW
                     TITSANIDRLRVTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFSIRMRRMTPDSITDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQNCDQSVPDGFGGTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPIWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIKPGHDYYFYVRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLTEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKRLTAPTITSGGNP
                     PAFSLTSDGRLTAKNADISGSVNANSGTLNNVTINQNCTIKGMLEATQVRGDFVKAVS
                     KAFPKKVGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIFNGIAYDDPGSGNNPGGT
                     RYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGRGSVTLEFKIFQKGNQGAGN
                     ITDCTVIVTKKAASGISIR"
     gene            21682..22281
                     /locus_tag="RHV13_002027"
     CDS             21682..22281
                     /locus_tag="RHV13_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family protein"
                     /protein_id="HDV1241560.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKKHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            22346..25372
                     /locus_tag="RHV13_002028"
     CDS             22346..25372
                     /locus_tag="RHV13_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV1241561.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHATDAADSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSVAATSAGAAKTSETNAAASEQSA
                     ATSASTATTKASEAATSARDAAASKEAAKSSETNASLSASSAASSATAAGNSAKAAKT
                     SETNARSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQATAAARSASAAKTSETNAKASETRAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSAKAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYDLASGKYTAQDATTA
                     QKGIIQLSSATNSTSETLAATPKAVKAANDNAEKRLQKDQNGADIPGKDTFTKNIGAC
                     RAFGGSVSTTTGNWTTAQFIEWLDSQGAFNHPYWMCKGSWSYGNNKIITDTGCGNIHL
                     AGAVIEVMGIKSAMTIRITTPTTSSGGGTTNAQFTYINHGTDYSPGWRRDYNSRNKPT
                     ASEIGALPSGGTAVSSVNLASKGRVAALTDNTQGAAGLELYEVYNNGYPTTYGNIIHL
                     KGMTAVGEGELLIGWSGTSGAHAPAFIRSRRDTTDANWSPWAQLYTSAHPPAEFYPVG
                     APIPWPSDTVPSGYVLMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVL
                     SQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTNNIGAHTHSVSGTAASAGNHTH
                     SVTGASAVSQWSQNGSVHKVVSAASVNTSAAGAHTHSVSGTAASAGAHAHTVGIGAHT
                     HSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            25369..26271
                     /locus_tag="RHV13_002029"
     CDS             25369..26271
                     /locus_tag="RHV13_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HDV1241562.1"
                     /translation="MITLILSAPVTEMAEAFKRVFANADNVNIVGKPFETIREFDCMV
                     SAANSFGLMDGGVDAAITTFFGSQLQARVQNHILREYLGEQPVGSAFVIETGHNYHPW
                     LVHAPTMRVPLTIDGTDAVYNATWAALLAIFQHNKNATTDRKIKTVVFPAMGAGCGQV
                     PFESVARQMKQAWDNFNKKTESINWEYAQSRQSAVFGTYAYCPGNSVCRYADTKYIGC
                     GDYRTYCSRSGQVCINPEHQADDVLIQHQANNRFRPGSHIHRINPENPVGNVISGAHS
                     HGSSIVIGAHTHTLNKQYSVSDIK"
     gene            26280..>26814
                     /locus_tag="RHV13_002030"
     CDS             26280..>26814
                     /locus_tag="RHV13_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDV1241563.1"
                     /translation="MDFRMSEQPRTITIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDESSWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEFQKWNGTAWVKDTEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEKE
                     TLLLEAWKKYRVLLNRVD"
BASE COUNT         6396 a         6692 c         8072 g         5654 t
ORIGIN      
        1 ggttcaattt aaatcggcta aaaataacac tggaaattat ttgttggtta tttgttgaga
       61 tttgcttatg tatttgtagt ggtgttttca atactcggta gcattctcgc aaatatcatt
      121 ttgtggttta cgtacgtaaa aaattggtta tgctgttaag agtggttact tcgtcacaca
      181 gcttaaaccc gccgtcgagc gggtttttcc attttttgag tctcgatatt agctgataac
      241 ccaatacctg agttattcac tgactccgag tctgttacgt ttctgctttt ttgcgatacg
      301 ttgtattccc tcaatttaca cccgctttgt ctgcgaggtg gggttatgaa atccatggat
      361 aagttaacaa cgggtgtcgc ctatggcacc tcagcaggta gtgccggtta ctggttttta
      421 cagctgctcg ataaagtcac gccctcacag tgggcagcaa taggtgtgct gggtagcctg
      481 gtatttggcc tgctgacgta cctgacaaac ctttatttca agattaaaga agataagcgc
      541 aaggctgcga gaggtgaata atgcctccat cattacgaaa agccgttgct gctgctattg
      601 gtggcggagc aattgctata gcatcagtgt taatcactgg cccaagtggt aacgatggtc
      661 tggaaggtgt cagctacata ccatacaaag atattgttgg tgtatggact gtatgtcacg
      721 ggcatacagg aaaagacatc atgctcggta aaacgtatac caaagcagaa tgcaaagccc
      781 tcctgaataa agaccttgcc acggtcgcca gacaaattaa cccgtacatc aaagtcgata
      841 taccggaaac aatgcgcggc gctctttact cattcgttta caacgtgggt gctggcaatt
      901 tcagaacatc gacgcttctt cgcaaaataa accagggcga tatcaaaggc gcatgtgatc
      961 agctacgtcg ctggacatat gctggcggta agcaatggaa aggtctcatg actcgtcgtg
     1021 agattgagcg tgaaatctgt ttgtggggtc agcaatgaac agagtaaccg cgattatctc
     1081 cgctctggtt atctgcatca tcgtctgcct gtcatgggct gttaatcatt accgtgataa
     1141 cgccattacc tacaaagccc agcgcgacaa aaatgccaga gaactgaagc tggcgaacgc
     1201 ggcaattact gacatgcaga tgcgtcagcg tgatgttgct gcgctcgatg caaaatacac
     1261 gaaggagtta gctgatgcga aagctgaaaa tgatgctctg cgtgatgatg ttgccgctgg
     1321 tcgtcgtcgg ttgcacatca aagcagtctg tcagtcagtg cgtgaagcca ccaccgcctc
     1381 cggcgtggat aatgcagcct ccccccgact ggcagacacc gctgaacggg attatttcac
     1441 cctcagagag aggctgatca ctatgcaaaa acaactggaa ggaacccaga agtatattaa
     1501 tgagcagtgc agatagagct gcccatatcg atgggcaact catgcaatta ttgtgagcaa
     1561 tacacacgcg cttccagcgg agtataaatg cctaaagtaa taaaaccgag aaatccattt
     1621 acgaatgttt gctgggtttc tgttttaaca acattttctg cgccgccaca aattttggct
     1681 gcatcaacag ttttctcctg tccaattccc gaaacgaaga aatgatgggt gatggtttcc
     1741 tttggtgtta ctgctgtcgg tttgtttcca acagtaaacg tctgttgagc acatcctgta
     1801 ataagcattg ccagagcggc agaaaacaac atttttttca tcttattatc ctgcattgtt
     1861 aaaaacggca gaatcctatg tgacaacaat taaacgatag ttaaatggat tgatgaaaat
     1921 taaaactata taggtggatg ctcagcctat tggaggaggg gggcactcag aatcctgtgg
     1981 aatgaaataa accgctctat ctgtccatta cccttttagc tgcgctgtat cgtcgccgta
     2041 ttcccgcatt aaccatgacc gtagcccgac ggggaattcc ttctgcgtga gtgtgcggga
     2101 ataatcaaaa acgatgcaca ccgggtttta ctgtgctgac agacgcaggg ttaccctcat
     2161 agtcgctttt ccggtgcgat ggtggaagaa accgggatgt tcatccatca tcactttgga
     2221 ttgatgtata tgctctcttt tctgacgtta gtctccgacg gcaggcttca atgacccagg
     2281 ctgagaaatt cccagaccct ttttgctcaa gagcgatgtt aatttgttca atcatttggt
     2341 taggaaagcg gatgttgcgg gttgttgttc tgcgggttct gttcttcgtt gacatgaggt
     2401 tgccccgtat tcagtgtcgc tgatttgtat tgtctgaagt tgtttttacg ttaagttgat
     2461 gcagatcaat taatacgata cctgcgtcat aattgattat ttgacgtggt ttgatggcgt
     2521 agatgcacgt tgtgacatgt agatgataat tattatcatt tttgcgggtc ctttccggcg
     2581 atccgacagg ttacggggcg gcgacctcgc gggttttcgc tatttatgaa aattttccgg
     2641 tttaaggcgt ttccgttctt cttcgtcata acttaatgtt tttatttaaa ataccctctg
     2701 aaaagaaagg aaacgacagg tgctgaaagc gagctttttg gcctctgtcg tttcctttct
     2761 ctgtttttgt ccgtggaatg aacaatggaa gtcaacaaaa agcagctggc tgacattttc
     2821 ggtgcgagta tccgtaccat tcagaactgg caggaacagg gaatgcccgt tctgcgaggc
     2881 ggtggcaagg gtaatgaggt gctttatgac tctgccgccg tcataaaatg gtatgccgaa
     2941 agggatgctg aaattgagaa cgaaaagctg cgccgggaag ttgaagaact gcggcaggcc
     3001 agcgagacag atctccagcc agggactatt gagtacgaac gccatcgact tacgcgtgcg
     3061 caggccgacg cacaggagct gaaaaatgcc agagactccg ctgaagtggt ggaaaccgca
     3121 ttctgtactt tcgtgctgtc gcggatcgca ggtgaaattg ccagtattct cgacgggatc
     3181 cccctgtcgg tgcagcggcg ttttccggaa ctggaaaacc gacatgttga tttcctgaaa
     3241 cgggatatca tcaaagccat gaacaaagca gccgcgctgg atgaactgat accggggttg
     3301 ctgagtgaat atatcgaaca gtcaggttaa caggctgcgg cattttgtcc gcgccgggct
     3361 tcgctcactg ttcaggccgg agccacagac cgccgttgaa tgggcggatg ccaattacta
     3421 tctcccaaaa gaatccgcat accaggaagg gcgctgggaa acactgccct ttcagcgggc
     3481 catcatgaat gcgatgggca gcgactacat ccgtgaggtg aatgtggtga agtctgcccg
     3541 tgtcggttat tccaaaatgc tgctgggtgt ttatgcctac tttatagagc ataagcagcg
     3601 caacaccctt atctggttgc cgacggatgg tgatgccgag aactttatga aaacccacgt
     3661 tgagccgacc atccgcgata ttccgtcgct gctggcgctg gctccgtggt atggcaaaaa
     3721 gcaccgggat aacacgctca ctatgaagcg tttttccaat ggtcgtggct tctggtgcct
     3781 gggcggtaaa gcggcaaaaa actaccgtga aaagtcggtg gatgtggcgg gttatgatga
     3841 acttgctgcc tttgatgagg atattgaaca ggaaggctct ccgacgttcc ttggcgacaa
     3901 acgtattgaa ggctcggtct ggccaaagtc catccgtggc tccacgccca aagtgagagg
     3961 cacctgccag attgagcgtg cagccagtga atccccgcat tttatgcgtt ttcatgttgc
     4021 ctgcccgcac tgcggggagg agcagtatct taaatttggc gacaaagaga cgccgtttgg
     4081 cctcaaatgg acgccggatg acccctccag cgtgttttat ctctgcgagc ataatgcctg
     4141 cgtcatccgc cagcaggagc tggactttac tgatgcccgt tatatctgcg aaaagaccgg
     4201 gatctggacc cgtgatggca ttctctggtt ttcgtcatcc ggtgaagaga ttgagccgcc
     4261 ggacagtgtg acctttcaca tctggacggc gtacagcccg ttcaccacct gggtgcagat
     4321 tgtcaaagac tggatgaaga cgaaagggga tacgggaaaa cgtaaaacct tcgtgaacac
     4381 cacgctcggt gagacatggg aagcgaaaat tggcgaacgt ccggatgctg aggtgatggc
     4441 ggagcggaaa gagcattatt cagcgcccgt tcctgaccgt gtggcttacc tgaccgccgg
     4501 tatcgactcc cagctggacc gctacgaaat gcgcgtatgg ggatgggggc cgggtgagga
     4561 aagctggctg attgaccggc agattattat gggccgccac gacgatgaac agacgctgct
     4621 gcgtgtggat gaggccatca ataaaaccta tacccgccgg aatggtgcag aaatgtcggt
     4681 atcccgtatc tgctgggata ctggcgggat tgacccgacc attgtgtatg aacgctcgaa
     4741 aaagcatggg ctgttccggg tgatccccat taaaggggca tccgtctacg gaaagccagt
     4801 ggccagcatg ccacgtaagc gaaacaaaaa cggggtttac cttaccgaaa tcggtacgga
     4861 taccgcgaaa gagcagattt ataaccgctt cacactgacg ccggaagggg atgaaccgct
     4921 tcccggtgcc gttcacttcc cgaataaccc ggatattttt gatctgaccg aagcgcagca
     4981 gctgactgct gaagagcagg tcgaaaaatg ggtggatggc aggaaaaaaa tactgtggga
     5041 cagcaaaaag cgacgcaatg aggcgctcga ctgcttcgtt tatgcgctgg cggcgctgcg
     5101 catcagtatt tcccgctggc agctggatct cagtgcactg ctggcgagcc tgcaggaaga
     5161 ggatggtgca gcaaccaaca agaaaacact ggcagattac gcccgtgcct tatccggaga
     5221 ggatgaatga cgcgacagga agaacttgcc gctgcccgtg cggcactgca tgacctgatg
     5281 acaggaaaac gggtggcaac ggtacagaaa gacggacggc gagtggagtt tacggccact
     5341 tccgtgtctg acctgaaaaa atacattgcg gagctggaag tgcagaccgg catgacacag
     5401 cgacgcaggg gacctgcagg attttatgta tgaaaacgtc ctccattccc acccttctgg
     5461 ggccggacgg catgacatcg ctgcgtgaat atgccggtta tcacggcggt ggcagcggat
     5521 ttggtgggca gttgcgggcg tggaacccac cgagtgaaag tgtggatgca gccctgttgc
     5581 ccaactttac ccgtggcaat gcccgcgcgg acgatctggt acgcaataac ggctatgccg
     5641 ccaacgccat ccagctgcat caggatcata tcgtcgggtc ttttttccgg ctcagtcatc
     5701 gcccaagctg gcgctatctg ggcatcgggg aggaagaagc ccgtgccttt tcccgcgagg
     5761 ttgaagcggc atgtaaagag tttgccgagg atgactgctg ctgcattgac gttgagcgaa
     5821 aacgcacgtt taccatgatg attcgggaag gtgtggccat gcacgccttt aacggtgaac
     5881 tgttcgttca ggccacctgg gataccagtt cgtcgcggct tttccggaca cagttccgga
     5941 tggtcagccc gaagcgcatc agcaacccga acaataccgg cgacagccgg aactgccgtg
     6001 ccggtgtgca gattaatgac agcggtgcgg cgctgggata ttacgtcagc gaggacgggt
     6061 atcctggctg gatgccgcag aaatggacat ggataccccg tgagttaccc ggcgggcgtg
     6121 cctcgttcat tcacgttttt gaacccgtgg aggacgggca gacccgcggt gcaaatgtgt
     6181 tttacagcgt gatggagcag atgaagatgc tcgacacgct gcagaacacg cagctgcaga
     6241 gcgccattgt gaaggcgatg tatgccgcca ccattgagag tgagctggat acgcagtcag
     6301 cgatggattt tattctgggc gcgaacagtc aggagcagcg ggaaaggctg accggctgga
     6361 ttggtgaaat tgccgcgtat tacgccgcag caccggtccg tctgggaggc gcaaaagtgc
     6421 cgcacctgat gccgggggac tcactgaacc tgcagacggc tcaggacacg gataacggct
     6481 actccgtgtt tgaacagtca ctgttgcggt atatcgctgc cgggctgggt gtctcgtatg
     6541 agcagctttc ccggaattac gcccagatga gctactccac ggcacgggcc agtgcgaacg
     6601 agtcgtgggc gtactttatg gggcggcgaa aattcgtcgc atcccgtcag gcgagccaga
     6661 tgtttctgtg ctggctggaa gaggccatcg ttcgccgcgt ggtgacgtta ccttcaaaag
     6721 cgcgtttcag ctttcaggaa gcccgcagcg cctgggggaa ctgtgactgg ataggctccg
     6781 gtcgtatggc catcgatggt ctgaaagaag tacaggaagc ggtgatgctg atagaagccg
     6841 gactgagtac ctacgagaaa gagtgcgcaa aacgcggtga cgactatcag gaaatttttg
     6901 cccagcaggt ccgtgaaacg atggagcgcc gtgcagccgg tcttaaaccg cccgcctggg
     6961 cggctgcggc atttgaatcc ggactgcgac aatcaacaga ggaggagaag agtgacagca
     7021 gagctgcgta atctcccgca tattgccagc atggctttta atgagccgct gatgcttgaa
     7081 cccgcctatg cgcgggtttt cttttgtgcg cttgcaggcc agcttgggat cagtcgcctg
     7141 acggatgcag tatccggcga cagcctgact gccggagagg cacccgcggc gctggcgtta
     7201 tccggtgatg atgacggacc acgacaggcc cgcagttatc aggtcatgaa cggcatcgcc
     7261 gtgctgccgg tgtccggtac gctggtcagc cggacgcggg cgctgcagcc gtattcggga
     7321 atgaccggtt acaacggcat tatcgcccgt ctgcaacagg ctgccagcga tccgatggtg
     7381 gacggcattc tgctcgatat ggacacaccg ggcgggatgg tggcgggagc atttgactgt
     7441 gctgacatca tcgcccgtgt gcgtgacata aagccggtat gggcgctggc caacgacatg
     7501 aactgcagtg caggtcagct gcttgccagc gccgcctccc ggcgtctggt cacgcagacc
     7561 gcccggacag gctccatcgg cgtcatgatg gctcacagta attacggcgc tgcgctggag
     7621 aaacagggcg tggaaatcac gctgatttac agcggcagcc ataaggtgga tggcaacccc
     7681 tacagccatc taccgggtga tgtccgggag acactgcagt cccggatgga tgcaacccgc
     7741 cggatgtttg cgcagaaggt gtcggcatat accggcctgt ccgtgcaggc tgtgctggat
     7801 accgaggctg cagtgtacag cggtcaggag gccattgatg ccggactggc tgatgaactt
     7861 gtcaacagca ccgatgcgat caccgttatg cgtgatgcac tggatgcacg taaatcccgt
     7921 ctctcaggag ggcgaatgac caaagagact caatcaacaa ctgtttcagc cactgcttcg
     7981 caggctgacg ttactgacgt ggtgcaagcg acggagggcg aaaacgccag cgcggcgcag
     8041 ccggacgtga acgcgcagat caccgctgcg gttgcggcag aaaacagccg cattatgggg
     8101 atcctcaact gtgaggaagc tcacggacgc gaagaacagg cacgtgtgct ggccgaaacc
     8161 cccggtatga ccgtggaaac ggcccgccgc attctggcag ctgcaccaca gagtgcacag
     8221 gcgcgcagtg acactgcgct ggatcgtctg atgcaggggg caccggcacc actggctgca
     8281 ggtaacccgg catctgatgc cgttaacgat ttgctgaaca caccagtgta agggatgttt
     8341 atgacgagca aagaaacctt tacccattac cagccgctgg gcaacagtga cccggcacat
     8401 acggcaaccg cgcccggcgg attgagtgcg aaagcgcctg caatgacccc gctgatgctg
     8461 gacacctcca cccgtaagct ggttgcgtgg gatggcacca ccgacggtac tgccgttggc
     8521 attctggcgg ttgatgctga ccagaccagc accacgctga cgttctacaa gtccggcacg
     8581 ttccgttatg aggatgtgct ctggccggag gctgccagcg acgagacgaa aaaacggacc
     8641 gcgtttgccg gaacggcaat cagcatcgtt taaccttacc cttcatcact aaaggccgcc
     8701 tgtgcggctt tttttacggg atttttttat gtcgatgtac acaaccgccc agctgctggc
     8761 ggcaaatgag cagaaattta agtttgatcc gctgtttctg cgtctctttt tccgtgagag
     8821 ctatcccttc accacggaga aagtctatct ctcacaaatt ccggggctgg taaacatggc
     8881 gctgtacgtt tcgccgattg tttccggtga ggttatccgt tcccgtggcg gctccacctc
     8941 tgaatttacg ccgggatatg tcaaacccaa gcatgaggtg aatccgcaga tgaccctgcg
     9001 tcgcctgccg gatgaagatc cgcagaatct ggcggacccg gcttaccgcc gccgtcgcat
     9061 catcatgcag aacatgcgag acgaagagct ggccattgct caggtcgaag agatgcaggc
     9121 agtttctgct gtgcttaagg gcaaatacac catgaccggt gaagccttcg atccggttga
     9181 ggtggatatg ggccgcagtg cggcgaacaa catcacacag tccggtggta cggagtggag
     9241 caagcgtgac aagtccacgt atgacccgac cgacgatatc gaagcctatg cgctgaacgc
     9301 cagcggcgtg gtgaatatca tcgtgtttga tccgaaaggc tgggcgctgt tccgttcctt
     9361 caaagccgtc aaggagaagc tggatacccg tcgcggctct cattccgagc tggagacagc
     9421 ggtaaaagac ctgggcgaag cggtgtccta taaggggatg tatggcgata cggcgatcgt
     9481 cgtgtattcc ggacagtacg tggaaaacga cgtcaaaaag aacttcctgc cggacaacac
     9541 gatggtgctg gggaacactc aggcacgcgg tctgcgcacc tatggctgca ttcaggatgt
     9601 ggacgcacag cgcgaaggta ttaacgcctc tgcccgctac ccgaaaaact gggtgaccac
     9661 cggcgatccg gcgcgtgagt tcaccatgat tcagtcagca ccgctgatgc tgctggctga
     9721 tcctgatgcg ttcgtgtccg tacaactggc gtaatcatgg cccttcgggg ccattttctc
     9781 tctgtggagg agtccatgac gaaagatgaa ctgattgccc gtcttcaggt gctgggtgag
     9841 caactgaacc gtgatgtcag cctgacgggg acgaaagaag aactggtgct ccgtgtggca
     9901 gagctggaag aggagcttga tgacacggat gacgctgccg gtcaggacac atctgtcagc
     9961 ccggaaaatg cgctgaccgg acatgaaaat gaggtggtat cagcgcagcc ggataccgtg
    10021 attgatacgg ctgctctggt cacggtcgtg gcactggtga cgctgcatac tgatgcactt
    10081 cacgccacgc gggatgagcc tgtggcattt gtgctgccgg gaacggcgtt tcgtgtctct
    10141 gccggtgtgg cagccgaaat gacagaacat ggcctggcca gaatgcaata acgggaggcg
    10201 ctgtggctga ttccgataac ctgttcgatg ctgccattgc ccgcgccgat gaaacgatac
    10261 gcgggtacat gggaacgtca gccaccatga catccggtga gctgtccggt gctgtgatac
    10321 gtggtgtttt tgatgaccct gaaaatatca gctatgccgg acagggggtg cgcgttgaag
    10381 gctccagccc gtccctgttt gtccggactg atgatgtgcg gcagctgcgg cgtggagaca
    10441 cactgaccat cggcgaggaa aacttctggg tggaccggat ttcgccggat gatggcggaa
    10501 gctgtcatct ctggcttggg cgtggcgtgc cgcctgccgt taaccgtcgc cgctgaaagg
    10561 gggatgtatg gccataaaag gtcttgagca ggccgttgaa aacctcagcc gtatcagcaa
    10621 aacggcggtg cctggtgccg ccgcaatggc cattaaccgc gttgcgtcat ccgcgatatc
    10681 gcagtctgcg tcacaggttg cccgtgagac aaaggtacgc cggaaactgg taaaggaaag
    10741 ggccaggctg aaaagggcca cggtcaaaaa tccgcaggcc agaatcaggg ttaaccgggg
    10801 ggatttgccc gtaatcaagc tgggtaacgc gcgggttgtc ctttcccgcc gcagacgtcg
    10861 taaaaagggg cagcgttcat ccctgaaagg tggcggcagc gtgcttgtgg tgggaaaccg
    10921 tcgtattccc ggcgcgttta ttcagcaact gaaaaatggc cggtggcatg tcatgcagcg
    10981 tgtggccggg aaaaaccgtt accccattga tgtggtgaaa atcccgatgg cggtgccgct
    11041 gaccacggcg tttaaacaga atattgaacg gatacggcgt gaacgtcttc cgaaagagct
    11101 gggctatgcg ctgcagcatc aactgagaat ggtaataaag cgatgaaaca tactgaactc
    11161 cgtgcagccg tactggatgc actggagaag catgacaccg gggcgacgtt ttttaatggt
    11221 cgccccgctg tttttgatga ggcggatttt ccggcagttg ccgtttatct caccggcgct
    11281 gaatacacgg gcgaagagct ggacagcgat acatggcagg cggagctgca tattgaagtt
    11341 ttcctgcctg ctcaggtgcc ggattcagag ctggatgcgt ggatggagtc ccggatttat
    11401 ccggtgatga gtgatatccc ggcactgtca gatttgatca ccagtatggt ggccagcggc
    11461 tatgactacc ggcgcgacga tgatgcgggc ctgtggagtt cagccgatct gacttatgtc
    11521 attacctatg aaatgtgagg acgctatgcc tgtaccaaat cctgtaatgc cggtgaaagg
    11581 tgccgggacc accctgtggg tttataaggg aagcggtgac ccttatgcga atccgctttc
    11641 agacgttgac tggtcgcgtc tggcaaaagt taaagacctg acgcccggcg aactgaccgc
    11701 tgagtcctat gacgacagct atctcgatga tgaagatgca gactggactg cgaccgggca
    11761 ggggcagaaa tctgccggag ataccagctt cacgctggcg tggatgcccg gagagcaggg
    11821 gcagcaggcg ctgctggcgt ggtttaatga aggggatacc cgagcctata aaatccgctt
    11881 cccgaacggc acggtcgatg tgttccgcgg ctgggtcagc agtatcggta aggcggtgac
    11941 ggcgaaggaa gtgatcaccc gcacggtgaa agtcaccaac gtgggccgtc cgtcgatggc
    12001 agaagatcgc agcacggtga cggcgacaac cggcatgact gtgacgcctg ccagcacctc
    12061 ggtggtgaaa gggcagagca ccacgctgac cgtggcattc cagccggaag gcgcaaccga
    12121 caagagcttc cgtgcggtgt ctgcggataa aacaaaagcc accgtgtcgg tcagtggtat
    12181 gaccatcacc gtgaaaggtg ttgctgcagg caaggtcaac attccggtcg tatccggtaa
    12241 tggtgaactt gctgcggttg cagaaatcac cgtcaccgcc agttaatccg gggagtcagc
    12301 gatgttcctg aaaaccgaat catttgaaca taacggtgtg accgtcacgc tttctgaact
    12361 gtcagccctg cagcgaattg agcatctcgc cctgatgaaa cggcaggcag aacaggcgga
    12421 gtcagacagc aaccggaagt ttactgtgga agacgccatc agaaccggcg cgtttctggt
    12481 ggcgatgtcc ctgtggcata accatccgca gaagacgcag atgccgtcca tgaatgaagc
    12541 cgttaaacag attgagcagg aagtgcttac cacctggccc acggaggcaa tttctcatgc
    12601 tgaaaacgtg gtgtaccggc tgtctggtat gtatgagttt gttgtgaata atgcccctga
    12661 acagacagag gacgccgggc ccgcagagcc tgtttctgcg ggaaagtgtt cgacggtgag
    12721 ctgagttttg ccctgaaact ggcgcgagag atggggcgac ccgactggcg tgccatgctt
    12781 gccgggatgt catccacgga gtatgccgac tggcaccgct tttacagtac ccattatttt
    12841 catgatgttc tgctggatat gcacttttcc gggctgacgt acaccgtgct cagcctgttt
    12901 ttcagcgatc cggatatgca tccgctggat ttcagtctgc tgaaccggcg tgaggctgac
    12961 gaagagcctg aagatgatgt gctgatgcag aaagcggcag ggcttgccgg aggcgtccgc
    13021 tttggcccgg acgggaatga agttatcccc gcttccccgg atgtggcgga catgacggag
    13081 gatgacgtaa tgctgatgac agtatcagaa gggatcgcag gaggagtccg gtatggctga
    13141 accggtaggc gatctggtcg ttgatttaag tctggatgcg gccagatttg acgagcagat
    13201 ggccagagtc aggcgtcatt tttccggtac ggaaactgat gcgaaaaaaa cagcggcagt
    13261 cgttgaacag tcgctgagcc gacaggcgct ggctgcacag aaagcgggga tttccgtcgg
    13321 gcagtataaa gccgccatgc gtatgctgcc tgcacagttc accgacgtgg ccacgcagct
    13381 tgcaggcggg caaagcccgt ggctgatcct gctgcaacag ggggggcagg ttaaggactc
    13441 cttcggcggg atgatcccca tgttcagggg gcttgccggt gcgatcaccc tgccgatggt
    13501 gggggccacc tcgctggcgg tggcgaccgg tgcgctggcg tatgcctggt atcagggcaa
    13561 ctcaaccctg tccgatttca acaaaacgct ggtcctttcc ggcaatcagg cgggactgac
    13621 ggcagatcgt atgctggtcc tgtccagagc cgggcaggcg gcagggctga cgtttaacca
    13681 gaccagcgag tcactgacgg cgctggtgaa tgccggtgtg cgtggtggtg agcagtttga
    13741 ggcgatcagc cagagtgtgg cgcgtttctc ctctgcatcc ggcgtggagg tggacaaggt
    13801 cgctgaagcc ttcgggaagc tgaccacaga cccgacgtcg ggactgacag cgatggcacg
    13861 tcagttccat aacgtgacgg cggagcagat tgcgtatgtt gctcagttgc agcgttccgg
    13921 agatgaagcc ggggcattgc aggcggcgaa cgaggccgca acgaaagggt ttgatgacca
    13981 gacccgccgc ctgaaagaga acatgggcac gctggagacc tgggcagaca ggacagcacg
    14041 ggcattcaaa tccatgtggg attcggtgct ggatattggt cgcccggaca ctgcccaggg
    14101 aatgctggag aaagcagaaa aggcttttga tgaggcggac aaaaaatggc agtggtatca
    14161 gagccggagc caccggcgcg gtaaaacctc agcatttctt gccaatctcc ggggagcatg
    14221 ggaggacaga gcgaatgcgc aacttgggct ttcagccgcc acgttgcagg ccgatcttga
    14281 aaaggccaga gagatggcag caaaggactg ggccgagtct gaggcatcac ggctgaaata
    14341 taccgaagag gcgcagaagg cttacgaacg gctgcagacg ccgctggaga aatataccgc
    14401 ccgtcaggaa gaactgaaca aggcactgaa agacgggaaa atcctgcagg cggattacaa
    14461 cacgctgatg gcggcggcga aaaaggatta tgaagcgacg ctgaaaaagc cgaaacagtc
    14521 cggcgtgaag gtgtctgcgg gcgatcgtca ggaagacagt gctcatgctg ccctgctgac
    14581 gcttcaggct gaactccgga cgctggagaa gcatgccgga gcaaatgaga aaatcagcca
    14641 gcagcgccgg gatttgtgga aggcggagag ccagttcgcg gtactggagg aggcggcgca
    14701 acgtcgccag ctgtctgcac aggagaaatc cctgctggcg cataaagatg agacgctgga
    14761 gtacaaacgc cagctggctg cacttggcga caaggttacg tatcaggagc gcctgaacgc
    14821 gctggcgcag caggcggata aattcgcaca gcagcaacgg gcaaaacggg ccgccattga
    14881 tgcgaaaagc cgggggctga ctgaccggca ggcagaacgg gaagccacgg aacagcgcct
    14941 gaaggaacag tatggcgata atccgctggc gctgaataac gtcatgtcag agcagaaaaa
    15001 gacctgggcg gctgaagacc agcttcgcag gagctggatg gcaggcctga agtccggctg
    15061 gagtgagtgg gaagagagcg ccacggacag tatgtcgcag gtaaaaagtg cagccacgca
    15121 gacctttgat ggtattgcac agaatatggc ggcgatgctg accggcagtg agcagaactg
    15181 gcgcagcttc acccgttccg tgctgtccat gatgacagaa attctgctta agcaggcaat
    15241 ggtggggatt gtcgggagta tcggcagcgc cattggcggg gctgttggtg gcggcgcatc
    15301 cgcgtcaggc ggtacagcca ttcaggccgc tgcggcgaaa ttccattttg caaccggagg
    15361 atttacggga accggcggca aatatgagcc agcggggatt gttcaccgtg gtgaatttgt
    15421 cttcacgaag gaggcaacca gccggattgg cgtggggaat ctttaccggc tgatgcgcgg
    15481 ctatgccacc ggcggttatg tcggtacacc gggcagcatg gcagacagcc ggtcgcaggc
    15541 gtccgggacg tttgagcaga ataaccatgt ggtgattaac aacgacggca cgaacggtca
    15601 gataggtccg gctgctctga aggcggtgta tgacatggcc cgcaagggtg cccgtgatga
    15661 aattcagaca cagatgcgtg atggtggcct gttctccgga ggtggacgat gaagaccttc
    15721 cgctggaaag tgaaacccgg tatggatgtg gcttcggtcc cttctgtaag aaaggtgcgc
    15781 tttggtgatg gctattctca gcgagcgcct gccgggctga atgccaacct gaaaacgtac
    15841 agcgtgacgc tttctgtccc ccgtgaggag gccacggtac tggagtcgtt tctggaagag
    15901 cacgggggct ggaaatcctt tctgtggacg ccgccttatg agtggcggca gataaaggtg
    15961 acctgcgcaa aatggtcgtc gcgggtcagt atgttgcgtg ttgagttcag cgcagagttt
    16021 gaacaggtgg tgaactgatg caggatatcc ggcaggaaac actgaatgaa tgcacccgtg
    16081 cggagcagtc ggccagcgtg gtgctctggg aaatcgacct gacagaggtc ggtggagaac
    16141 gttatttttt ctgtaatgag cagaacgaaa aaggtgagcc ggtcacctgg caggggcgac
    16201 agtatcagcc gtatcccatt caggggagcg gttttgaact gaatggcaaa ggcaccagta
    16261 cgcgccccac gctgacggtt tctaacctgt acggtatggt caccgggatg gcggaagata
    16321 tgcagagtct ggtcggcgga acggtggtcc ggcgtaaggt ttacgcccgt tttctggatg
    16381 cggtgaactt cgtcaacgga aacagtgacg ccgatccgga gcaggaggtg atcagccgct
    16441 ggcgcatcga gcagtgcagc gaactgagcg cggtcagtgc ctcttttgta ctgtccacgc
    16501 cgacggaaac ggacggtgcc gtttttccgg gacgtatcat gctggccaac acctgcacct
    16561 ggacctatcg cggtgatgag tgcggttatc acggtccggc ggtcgcggat gaatatgatc
    16621 agccgacgtc cgatatcacg aaggataaat gcagcaaatg cctgagcggc tgtaagtttc
    16681 gcaataacgt cggcaacttt ggcggcttcc tttccattaa caaactttcg cagtgaatcc
    16741 catgacagag acagaatcag cgattctggc gcacgcccgg cgatgtgcgc cagcggagtc
    16801 gtgcggcttc gtggtgagaa caccggaggg ggaaagatat tttccctgcg tgaatatctc
    16861 cggtgagccg gaggcgtatt tccggatgtc gccggaggac tggctgcggg cagagatgca
    16921 gggtgagatt gtggcgctgg tccacagcca ccccggtggt ctgccctggc tgagtgagac
    16981 cgaccggcgg ctgcaggtgc agagtgattt gccgtggtgg ctggtttgcc ggggggcgat
    17041 tcacaagttc cgctgtgtgc cacatctttc cgggcggcgc tttgagcacg gggtgacgga
    17101 ctgttacacg ctgttccggg atgcttacca tctggcgggg attgagatgc cggattttca
    17161 tcgcgaggat gactggtggc gtcacggtca gaatctctat ctggataatc tggaggccac
    17221 agggctgtat caggtgccgt tgtcatcagc acaaccgggc gatgtgctgc tgtgctgttt
    17281 tggttcatcg gtgccgaatc atgccgccat ttactgtggt gacagcgagc tgctgcacca
    17341 tattcctgaa caactgagca aacgagagag gtacaccgac aaatggcagc gacgcacaca
    17401 ctccctctgg cgtcaccggg aatggcacgc atctgccttt acggggattt gcaacgattt
    17461 ggccgccgca tcgaccttcg tgtgaaaacg ggggctgaag ccatccgcgc actggccaca
    17521 cagctcccgg cgtttcgtca gaaactgagc gacggctggt atcaggtacg gattgccggg
    17581 cgggacgtca gcacgtccgg gttaacggcg cagttacatg agactctgcc tgacggcgct
    17641 gtgattcata ttgttcccag agtcgccggg gccaagtcag ggggcgtatt ccagattgtt
    17701 ctgggggctg ccgccattgc cggatcattc tttactgccg gagccaccct tgcagcatgg
    17761 ggggcagcca ttggggccgg tggtatgacc ggcatcctgt tttctctcgg tgccagtatg
    17821 gtgctcggtg gtgtggcgca gatgctggca ccgaaagcca gaactccccg tacacagaca
    17881 acggataacg gtaagcagaa cacctatttc tcctcactgg ataacatggt tgcccagggc
    17941 aatgttctgc ctgttctgta cggggaaatg cgcgtggggt ctcgcgtggt ttctcaggag
    18001 atcagcacgg cagacgaagg ggatggtggt caggttgtgg tgattggtcg ctgatgaaaa
    18061 acgtttatgt gaaaccgcct ccgggcggtt ttgtcgttta tggagcgtga ggaatgggta
    18121 aaggcagcag taaggggcat actccgcgcg aagcgaagga caacctgaag tccacgcagt
    18181 tgctgagtgt gatcgatgcc atcagcgaag ggccgattga aggtccggtg gatggattaa
    18241 aaagcgtgct gctgaacagt acgccagtgc tggacagtga ggggaatacc aacatctccg
    18301 gcgtcacggt ggtgttccgg gccggtgagc aggagcagac accgccggag ggatttgaat
    18361 cctccggctc cgagacggtg ctgggtacgg aagtgaaata cgacacgccg atcacctgga
    18421 ccatcacgtc ggcaaacatt gaccgactgc gtgttacctt cggcgtgcag gcactggtgg
    18481 aaaccacctc aaagggggac aggaatccgt cggaagtccg cctgctggtt cagatacagc
    18541 gtaatggtgg ctgggtgacg gaaaaagaca tcaccattaa gggcaaaacc acctcgcagt
    18601 atctggcctc ggtggtggtg ggtaacctgc cgccgcgccc gttcagtata cggatgcgca
    18661 ggatgacgcc ggacagcatc acagaccagc tgcagaacaa aacgctctgg tcgtcataca
    18721 ccgaaatcat cgatgtgaaa cagtgctacc cgaacacggc actggtcggt gtgcaggtgg
    18781 actcggagca gttcggcagc cagcaggtga gccgtaatta tcatctgcgc gggcgtattc
    18841 tgcaggtgcc gtcgaactat aacccgcaga cgcggcaata cagcggtatc tgggacggaa
    18901 cgtttaaacc ggcatacagc aacaacatgg cctggtgtct gtgggatatg ctgacccatc
    18961 cgcgctacgg catggggaaa cgtcttggtg cggcggatgt ggataaatgg gcgctgtatg
    19021 tcatcggcca gaattgcgac cagtcggtgc cggacggctt tggcggcacg gagccgcgca
    19081 tcacctgtaa tgcctggctg accacacagc gcaaggcgtg ggatgtgctc agtgatttct
    19141 gctcggcgat gcgctgtatg ccgatatgga acgggcagac gctgacgttc gtgcaggacc
    19201 gaccatcaga taaggtgtgg acctataacc gcagtaatgt ggtgatgccg gatgatggcg
    19261 cgccgttccg ctacagcttc agcgccctga aggaccgcca taatgccgtt gaggtgaact
    19321 ggattgaccc ggataacggc tgggagacgg cgacagagct tgtggaggac acgcaggcca
    19381 ttgcccgtta cggtcgtaac gtcacgaaga tggatgcctt tggctgtacc agtcgggggc
    19441 aggcgcaccg cgccgggctg tggctgatta aaacggaact gctggaaacg cagaccgtgg
    19501 acttcagcgt gggtgcggaa gggcttcgcc atgtaccggg ggatgtcatt gaaatctgcg
    19561 atgatgacta tgcgggtatc agcaccggcg ggcgcgtgct ggcggtgaac agccagaccc
    19621 ggacgctgac gctcgaccgt gaaatcacgc tgccatcctc cggtaccacg ctgataagcc
    19681 tggttgacgg aagtggcaat ccggtcagcg tggaggtcca gtccgtcacc gacggcgtga
    19741 aggtaaaagt gagccgtgtt cctgacggcg ttgccggata cagcgtgtgg gggctgaagc
    19801 tgccgacgct gcgccagcga ctgttccgct gcgtgagtat ccgtgagaac gacgacggca
    19861 cgtatgccat caccgccgtg cagcatgtgc cggaaaaaga ggccatcgtg gataacgggg
    19921 cgcactttga cggcgaccag agcggcacgg tgaatggtgt cacgccgcca gcggtgcagc
    19981 acctgactgc cgaagtcacc gcagacagcg gggaatatca ggtgctggcg cgctgggaca
    20041 caccgaaggt ggtgaagggc gtgagcttcc tgctccgtct gaccgtaaca gcggacgacg
    20101 gcagtgagcg gctggtcagc acggcccgga cgacggaaac cacataccgc ttcacacaac
    20161 tggcgttggg gaactacagg ctgacagtcc gggcggtaaa tgcgtgggga cagcagggcg
    20221 atccggcatc ggtatcgttc cggattgccg caccggcagc gccgtctcgg attgagctga
    20281 caccgggcta ttttcagata accgccacgc cgcatcttgc ggtttatgac ccgacggtac
    20341 agtttgagtt ctggttctcg gaaaaacgga ttgcggatat caggcaggtt gaaaccagcg
    20401 cgcgttatct tggtacggca ctgtactgga tagccgccag tatcaatatc aaaccgggcc
    20461 atgattatta tttttacgtt cgcagtgtga acaccgttgg caaatcggca ttcgtggagg
    20521 ctgtcggtca gccgagtgat gatgcatcag gctatctgga ttttttcaaa ggcgagatag
    20581 ggaaaaccca tctggctcag gagctgtgga cgcagattga taacggtcag cttgcgcctg
    20641 acctgactga aatcaggacg tccataacgg atgtcagcaa tgaaataaca cagaccgtca
    20701 ataagaaact ggaagaccag agtgcagcga tccagcagat acagaaggtt caggttgata
    20761 caaataataa cctgaacagc atgtgggcag tgaagctgca gcagatgcag gacggacgcc
    20821 tttatattgc gggtatcggt gccggtattg agaacacccc tgacggcatg cagagtcagg
    20881 tgctgctggc agcagacagg attgcgatga ttaatcctgc gaatggcaac acaaagccga
    20941 tgtttgttgg tcagggcgat cagatattca tgaatgaagt gttcctgaaa cgcctgacgg
    21001 cccccaccat taccagcggc ggtaatcctc cggcattttc cctgacatca gacgggagac
    21061 tgacggcgaa aaatgcggat atcagtggca gtgtgaatgc gaactcagga acgctcaaca
    21121 atgtcacgat taaccagaac tgtacgatta agggcatgct ggaggcgacc caggtcagag
    21181 gagatttcgt taaagctgta tcaaaagcct tcccgaaaaa agtcggtacg tggggtaaca
    21241 cggaaacacc aaacggtacg gttacagtca ccatcagcga tgatcataac tttgaccgcc
    21301 agattattat tccgcccatt atttttaacg gtatagcgta tgacgatccg gggagcggaa
    21361 ataacccagg aggcacgcga tacacgggtt atggttttga agttcgcaaa aacggcgtat
    21421 taatcgcatc cagagaaact aaaggggcca ttcccggtag ttacagtgca gttattgata
    21481 tgccgagtgg caggggaagc gtcactctgg agtttaagat tttccagaaa ggcaatcagg
    21541 gggcaggcaa tatcaccgac tgtacggtga ttgtgaccaa aaaagccgct tccggcatca
    21601 gtattcgttg aaatatttat aaccccaata aagggcgtca ggaatgacgc cttttttatt
    21661 gcagaaaagc gagaggtaat tatgcgtaaa gtttgtgcag caattttgtc cgcagccatt
    21721 tgtctggccg tatccggtgc gcctgcatgg gcgtctgaac atcagtccac gctgagcgcg
    21781 gggtatcttc atgcctcgac gaacgtcccc ggcagcgatg atctgaacgg gattaacgtg
    21841 aaataccgtt atgagtttac ggacacactg gggctggtga cgtcattcag ctatgcagga
    21901 gacaagaatc gccagctgac ccgttacagc gatacccgct ggcatgaaga ttccgttcgt
    21961 aaccgctggt tcagcgtaat ggcggggccg tctgtgcgcg tgaatgaatg gttcagcgcg
    22021 tatgcgatgg cgggtgtagc ttacagccgt gtgtcgactt tctccgggga ttatcttcgc
    22081 gtaactgaca acaaggggaa aaagcacgat gtgctgaccg gaagtgatga cggtcgccac
    22141 agcaacacgt ctctggcgtg gggggctggc gtgcagttta acccgaccga atccgtggcc
    22201 attgatattg cttatgaagg ctccggcagt ggtgactggc gcactgacgg tttcatcgtg
    22261 ggtgtcggtt ataaattctg attagccagg taacacagtg ttatgacagc ccgccggttc
    22321 aggcgggctt ttttgtgggg tgaatatggc agtaaagatt tcaggtgtac tgaaagacgg
    22381 cacaggaaaa ccggtacaga actgcacaat ccagctgaaa gcaaaacgta acagcaccac
    22441 ggtggtggtg aacacgctgg cctcagaaaa tccggatgaa gccgggcgtt acagcatgga
    22501 cgttgagtac gggcagtaca gcgttattct gttggtggag ggattcccgc cgtcacatgc
    22561 cgggaccatc accgtgtatg aagattcccg acccggtacg ctgaatgatt ttctcggtgc
    22621 catgacggag gatgatgccc gtcctgaggc actgcgccgt tttgaactga tggtggaaga
    22681 ggtggcgcgt aacgcgtccg cggtggcaca gaacacggca gccgcgaaga agtcagccag
    22741 cgatgccagc acatcagccc gtgaggcggc aacccatgcg actgatgctg cagattcagc
    22801 acgtgcagcc agcacgtcag ccggacaggc cgcttcgtcg gctcagtcag cgtcttccag
    22861 cgcaggaacg gcatcaacaa aggccactga agcctcaaaa agtgctgccg ctgcagagtc
    22921 ctcaaaaagc gtggcagcta ccagtgccgg tgcggcgaaa acgtcagaaa cgaatgccgc
    22981 agcgtcagaa caatcagccg ccacttctgc atccaccgcg accacgaaag cgtcagaagc
    23041 tgccacctca gcccgggatg cggcggcctc aaaagaggca gcgaaatcat cagaaacgaa
    23101 cgcatccttg agcgccagta gcgcagcttc ctcggcaacg gcggcaggaa attccgcgaa
    23161 ggcggcaaaa acgtccgaga cgaacgccag gtcttctgag acggcagcgg gacagagcgc
    23221 ctcagctgcg gcaggctcaa aaacagcggc tgcgtcgtct gccagtgccg cgtcaacaag
    23281 tgccgggcag gcctcagcca gtgccaccgc cgccggaaaa tcggcagaaa gcgccgcatc
    23341 atccgcttca acagccacaa cgaaggctgg cgaagccact gagcaggcca cggcagcagc
    23401 gaggtcagct tccgcagcga agacatccga aacgaacgcg aaagcgtcgg aaacccgtgc
    23461 agaatcctca aaaacggcag cggcatcgtc cgccagttcg gcggcgtcat cggcatcatc
    23521 ggcgtctgct tcaaaagatg aggcgaccag acaggcgtca gcagcgaagg gcagcgccac
    23581 gacggcatcc acgaaggcga cagaggctgc tggtagtgcg aaggcagcag ctcagagcaa
    23641 aagtacggcg gaatctgcag caacgcgcgc tgagacagcg gcaaaacggg cagaggatat
    23701 tgcatccgcc gtggcgcttg aggatgcgag cacgacgaaa aaggggatag tacagctcag
    23761 tagtgcgact aacagcactt ccgagtcact ggcggcaaca ccaaaagccg ttaaggccgc
    23821 gtatgacctg gctagcggga aatacaccgc acaggatgca acgacagcac agaaagggat
    23881 aatccagcta agcagcgcga ccaacagcac gtctgaaacg ctggcggcaa cgccaaaggc
    23941 agtaaaagca gccaatgaca atgctgagaa acgtctgcag aaagatcaga acggtgcgga
    24001 tatccctggc aaagacacct ttacgaaaaa tattggtgcc tgccgtgcct tcggtgggtc
    24061 agtaagcaca acaacaggaa actggacgac tgcacagttt atcgagtggc tggattctca
    24121 gggagcattt aaccatccat actggatgtg caagggttcc tggtcttatg gcaataataa
    24181 aatcattact gatactggct gcggtaatat tcatctcgcc ggagctgtca ttgaagtaat
    24241 ggggataaag tcagcgatga cgatccgcat taccactccg accacatcca gcggtggcgg
    24301 aacaaccaac gcccagttta cctatattaa tcacggaaca gattattcac ctggctggcg
    24361 aagggactat aactccagaa ataagccaac ggcatcagag atcggggcgc taccgtcagg
    24421 tggaacagca gtatcatcag ttaatctggc ttcaaaaggt cgggtagccg cgctgacaga
    24481 caatacgcag ggggcagcag gtcttgagtt atacgaggtg tataacaacg gatatccaac
    24541 aacgtatgga aatatcattc acctgaaagg gatgacagcc gttggcgaag gtgagttact
    24601 catcggctgg agtggtacaa gcggtgctca tgctccggca tttattcgtt cacgacggga
    24661 tacgaccgac gcaaactggt cgccgtgggc gcagctttac acctcggctc atcctcctgc
    24721 agagttttat ccagtcggtg caccaatccc gtggccatca gataccgttc cgtctggtta
    24781 tgtcctgatg caggggcaga cttttgacaa atctgcatac ccgaaacttg cagccgctta
    24841 tccgtcaggc gtgatccctg atatgcgtgg ctggacgatt aagggcaaac ctgccagtgg
    24901 tcgggccgta ttgtctcagg aacaggacgg cattaaatcg cacacccaca gcgccagcgc
    24961 atccagtacg gatttgggga cgaaaaccac atcgtcgttt gattacggca ctaaatccac
    25021 gaataacatc ggggcgcata cgcacagtgt gagcggtaca gccgcaagtg ccggaaacca
    25081 tactcatagt gtcacaggcg catcagcagt cagccagtgg tcacaaaatg ggtcagtaca
    25141 taaggtagtg tctgcggcca gtgtgaatac aagtgctgca ggagcgcaca ctcatagtgt
    25201 cagcggcaca gctgcatctg caggtgctca cgcacatact gtcggtattg gtgctcatac
    25261 gcactcggtt gcgattggct cacatggaca caccatcacc gttaacgctg ctggtaacgc
    25321 ggaaaacacc gtcaaaaaca tcgcatttaa ctatattgtg aggcttgcat gattacgctc
    25381 attctttctg caccagtaac cgaaatggct gaagcattta agcgggtatt tgcaaatgca
    25441 gataatgtga atattgtcgg aaagccattt gaaacaatca gagaatttga ctgcatggta
    25501 agtgcggcaa atagtttcgg cctgatggat ggcggtgttg atgccgccat taccacattc
    25561 ttcggttctc agctacaggc ccgcgttcag aatcatattc ttcgtgaata tctcggcgag
    25621 cagcctgtag gttctgcatt tgttattgaa acggggcata attatcaccc ctggctggta
    25681 catgcgccaa caatgcgtgt tccgttgaca attgacggaa cagacgctgt atataacgca
    25741 acttgggcag ctctacttgc tatctttcag cacaataaaa acgcaacgac agacaggaaa
    25801 ataaagacgg tggtattccc tgcaatggga gccggatgtg gtcaggtgcc gtttgaaagc
    25861 gttgcccggc agatgaagca ggcatgggat aactttaata aaaaaacaga atcaattaac
    25921 tgggaatacg cacaatcccg ccagtcggca gtatttggca catatgcata ctgtccgggt
    25981 aattctgttt gccgttatgc ggatactaaa tatattggat gcggcgatta tcggacgtat
    26041 tgctcacgtt ccgggcaggt ctgtattaac cctgaacatc aggctgatga tgtactgata
    26101 caacatcagg ctaataaccg gtttcgccct ggttcgcata tacaccggat taatccagaa
    26161 aatcctgtag gtaatgtcat ctctggcgca catagccacg gaagtagcat cgttattggt
    26221 gctcacaccc atacgctcaa taaacaatat tctgtctctg atattaagta gaggtgaaca
    26281 tggatttcag aatgagtgaa caaccacgga ccataacaat ttataatctg ctggccggaa
    26341 ctaatgaatt tattggtgaa ggtgacgcat acattccgcc tcatacaggt ctgccagcaa
    26401 acagtaccga tattgcaccg ccagatattc cggctggttt cgtggctgtt ttcaacagtg
    26461 atgagtcatc gtggcatctc gttgaagatc atcggggtaa aacggtttat gacgtggctt
    26521 ccggcgacgc gttatttatt tctgaactcg gctcattacc ggaaaatgtc acctggttat
    26581 ccccggaagg ggagtttcag aagtggaacg gcacagcctg ggtgaaggat acggaagcag
    26641 aaaaactgtt ccggatccgg gaggcggaag aaacaaaaaa cagcctgatg caggtagcca
    26701 gtgagcatat agcgccgctt caggatgctg tggatctgga gatcgcaacg gagaaagaaa
    26761 ccttgttgct ggaagcctgg aaaaagtatc gggtgttgct gaaccgtgtt gata
//
LOCUS       DAPGOF010000056        26754 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.056, whole genome shotgun sequence.
ACCESSION   DAPGOF010000056 DAPGOF010000000
VERSION     DAPGOF010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.056"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(29..2899)
                     /locus_tag="RHV13_002031"
     CDS             complement(29..2899)
                     /locus_tag="RHV13_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HDV1241564.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(2896..3675)
                     /locus_tag="RHV13_002032"
     CDS             complement(2896..3675)
                     /locus_tag="RHV13_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HDV1241565.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAITDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANDIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(3726..5054)
                     /gene="ssnA"
                     /locus_tag="RHV13_002033"
     CDS             complement(3726..5054)
                     /gene="ssnA"
                     /locus_tag="RHV13_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HDV1241566.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDITLL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDTLA"
     gene            complement(5057..8155)
                     /gene="ygfK"
                     /locus_tag="RHV13_002034"
     CDS             complement(5057..8155)
                     /gene="ygfK"
                     /locus_tag="RHV13_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HDV1241567.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVHGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVADHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(8477..9055)
                     /gene="mocA"
                     /locus_tag="RHV13_002035"
     CDS             complement(8477..9055)
                     /gene="mocA"
                     /locus_tag="RHV13_002035"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HDV1241568.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     DHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            9158..9928
                     /gene="yqeC"
                     /locus_tag="RHV13_002036"
     CDS             9158..9928
                     /gene="yqeC"
                     /locus_tag="RHV13_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HDV1241569.1"
                     /translation="MKSIVDPSALFIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKCSCCVITVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            9976..11601
                     /locus_tag="RHV13_002037"
     CDS             9976..11601
                     /locus_tag="RHV13_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HDV1241570.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKSRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(11822..12754)
                     /gene="arcC"
                     /locus_tag="RHV13_002038"
     CDS             complement(11822..12754)
                     /gene="arcC"
                     /locus_tag="RHV13_002038"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDV1241571.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     VSFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(12802..14187)
                     /gene="hyuA"
                     /locus_tag="RHV13_002039"
     CDS             complement(12802..14187)
                     /gene="hyuA"
                     /locus_tag="RHV13_002039"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HDV1241572.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(14240..15451)
                     /locus_tag="RHV13_002040"
     CDS             complement(14240..15451)
                     /locus_tag="RHV13_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HDV1241573.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(15509..16705)
                     /gene="dpaL"
                     /locus_tag="RHV13_002041"
     CDS             complement(15509..16705)
                     /gene="dpaL"
                     /locus_tag="RHV13_002041"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HDV1241574.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(16763..17950)
                     /gene="ygeW"
                     /locus_tag="RHV13_002042"
     CDS             complement(16763..17950)
                     /gene="ygeW"
                     /locus_tag="RHV13_002042"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HDV1241575.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEQRTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            18429..20207
                     /locus_tag="RHV13_002043"
     CDS             18429..20207
                     /locus_tag="RHV13_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HDV1241576.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(20247..20726)
                     /gene="xdhC"
                     /locus_tag="RHV13_002044"
     CDS             complement(20247..20726)
                     /gene="xdhC"
                     /locus_tag="RHV13_002044"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HDV1241577.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(20723..21601)
                     /gene="xdhB"
                     /locus_tag="RHV13_002045"
     CDS             complement(20723..21601)
                     /gene="xdhB"
                     /locus_tag="RHV13_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HDV1241578.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPTLCAAASSIAG
                     PQIRNVATYGGNICNGATSGDSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(21612..23909)
                     /gene="xdhA"
                     /locus_tag="RHV13_002046"
     CDS             complement(21612..23909)
                     /gene="xdhA"
                     /locus_tag="RHV13_002046"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HDV1241579.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNM
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            24324..25079
                     /locus_tag="RHV13_002047"
     CDS             24324..25079
                     /locus_tag="RHV13_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDV1241580.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            25158..25231
                     /locus_tag="RHV13_002048"
     tRNA            25158..25231
                     /locus_tag="RHV13_002048"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25190..25192,aa:Gly,seq:ccc)
     gene            25465..>26754
                     /locus_tag="RHV13_002049"
     CDS             25465..>26754
                     /locus_tag="RHV13_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1241581.1"
                     /translation="MKGKSALTLLLAGIFSCGTCQTTGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLQNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTPD
                     QIKRMQDIISNPKRLDGIPGQMNAVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAINTGTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTS
                     SVALVTSGLERLSNLLSKKDSVFVSDLLREAKVNELDETLSTTRLNHLIDKGYERITL
                     QLDLGGESPGYLEKDKHYREADAALLNVIYPANLSKINTRRKEQVLKIVKKLAGPYGI
                     KRYEKDNYQSANFWFNDIKTDTDQNSHVKREKSFIPST"
BASE COUNT         6483 a         6546 c         6871 g         6854 t
ORIGIN      
        1 acaggccgcc gaagcggcca tttattattt atattttttc cagcgcagtg agtattttct
       61 ccggtgtgaa atgccattcg cgtaaccaga tgccgcatgc atcgtgaatt gcggtagcaa
      121 tcgccggagc tgcgccattt acaccgattt ccgagatcga tttcgccccg aacgggccga
      181 ctttatcgtc gctcggcacc agcacagcgc ggaaatcacg cggaatgtca ccaattttcg
      241 gtgcgccgta actgcgtaaa tcacgcgtta acgggtgacc ttcggcgtca taaatgatct
      301 cttcgctcat actgtggccg atagctcgca gggtagcacc gtagatttgt cccaatgcta
      361 attccgggtt gaccggtgta ccgcagtcca gcaaggcgta gaatttatcc aggcggattt
      421 cacccgtacg cgtgttgacg gcaacttcag cgaagtttgc gccatacggg aaggcgaaat
      481 caggcgtgat ataactgcca gttcccacca gtgaaccaaa gccggtgccg gtttcgcctt
      541 tatgggcaat atccccgaaa ctaacttcgc ctttcttgcc gcgcacgacg cccggcgttg
      601 ccagttgaac atctgccact gcctcaccca acatttgcgc gccgtggaac agaattttct
      661 cccgtagatt ttccgctgcc aaacgcgccg cgttacccga gaagcaagta ccggacgagg
      721 catatgcgcc tttatcaaac aacgcatgat cggtatcacc ggagataaca tgcacgtcct
      781 gtggtgggca gtgcagcact tctgctgcca gtttcgtcac cacagtatcc agaccagtac
      841 caatatccgc accgccagaa tgaacaataa aggtgccgtc cgattccagt ttgatcatgc
      901 agttagcctg atcgatatcc ggaatacccg atttctgcat gataatggcg acgccgcgac
      961 cgatgtgcca gtcaccattt tgcggtttcg gtgaagacca ttggatcatc tcgcgaccct
     1021 gacgcaggat ctcttccagt gcacagctgg cggcggaagg aacggaggtc ggcgctttac
     1081 cttcaccgat tgcaccgaga attttcagct cttgcccttc gtgtacccgg ttacgttcga
     1141 taatttccag ttggtcgatc tgtaactgtt cagcaagttc cgctaatgcc atggtgatag
     1201 cgaagttacc tttcggtgcg ccataaccct gataagcacc atttgggcaa atgttgctgt
     1261 agtaggtggt gacctggaaa tcgacgttat cgcacggata taacggcagc gacagcgccg
     1321 gtccgttaca cggtacggtg agtgagtggt tgccgtaagg gccagtgttg gcgcggaaat
     1381 ccatcttcac tgccgtcagg cgaccatctt ttttcgctcc cagtttgacg gtgactttcg
     1441 cgacgtgacg agaggtgtta gcaataaact cttcttcacg ggtgtagcgg aacagtaccg
     1501 gacgcccggt cacgcaggtt gcccaggcgc acacttcttc cagcaggatg tcctgtttgg
     1561 aaccaaaacc gccgccaact cgctctttaa tgacatgaac tttatgctgt ttcatgccca
     1621 cgaggcgcgc gacctggcgg cgtaagtgcc atggtacctg ggtggaggcg tggataacca
     1681 gacgatcgcc gtccatccgg gtaaagcaga tatgtgtttc agtcgggcac tgctgtgcct
     1741 gcgttgagtt ataggttcgc tcaatgatca catcggcatc ggcaaagcct ttgtccatat
     1801 cgccaatatg accatgaata ctggcggcga tatttttgcg agggcgagaa ccgatcggga
     1861 agttgatgat catatgctcg ccgcgctggg ctgcatggct gttatcgtct tccagagtat
     1921 ctggcgcacc agcaacatac accaccggtt catcgtgcac gacaggcgca tcttccgcca
     1981 ttgcttcgtc gatcgacatt accggcttaa gcacttcata ttcaacgtcg atgagcttca
     2041 gtgcttcgag cgcaatttct tcactttctg cgacgaccgc agcaacacga tcgccgacgt
     2101 gacgcatttt cttgccgaac atacggcggt caagcggtga cggttccggt gcgctctgac
     2161 cccccggggt atagtagata tccgggcaat tcaggtgagt aataacgtga acgacgcccg
     2221 gtaaggcttc agctttgctg acatccagat gagtaatcag tgcgtgagcg tgtgggctac
     2281 gtaacatttt aatgacgcag gcgtcagccg ttacgcggtc ttcaacatag cagggtttcg
     2341 cctgcaccat tttcgcggca tcagttttag gataatgctt gccaatgact tcaaggtcgt
     2401 cacggaaagt cggagcgata tcaatggtgg cctgcggatt atttttacgt gcaaccgcca
     2461 gttcaatgac ctgatagtat tgttgccagc ctgcatcgcg gctgaacaaa ccagaaagcg
     2521 cgtcgtcgat ctcttcgcgg gtaggtgcgg cgatgcgatc gagaagatcg gtgataatca
     2581 gagctgcagc tggatcgtta taaccagact gcaccacgcc aacatcaacc attgcctgtt
     2641 gaaccagact taactcgttc catttgccca gagattctgc ggtacgaata tctgccttct
     2701 ctaactgtgc ggcaataagc aaggacgcgt taacgatatt accgttaaag attattgcgt
     2761 cagaaccggc aaacccgaag ccatcatcac tgttgcgtac agagtgcatt cccatgttaa
     2821 acaacagctt ttgcacgttt tcgcctggat taacggttag ctcctgaggc gcgccattta
     2881 aagtaaagtg gatgatcata cggcttcctc cccagcctgt tggcagtcgg catacaggtc
     2941 ggctaccaga actcccgtga tatagcgttt ataggctacg ctgccccgca aatcttcctg
     3001 cgggaaaatg tcgttggcga cagcctgttc aagtgcattg ccttccagat tttgtttttc
     3061 gacatcgtgc agacgcagtg ctttactggc gacgccatcc agcgcaattc gcataccgtc
     3121 gtggtctgtt attgcaacgg ctgccgtcac gacggttaaa cctgcctgag aacggctaat
     3181 tttgcgggtc gcacaggtgc gatacggatc tttaatgata atttcggtta gcaggcgatc
     3241 gcatgggcag gccaggtagt cctcgattga cagcgtttcg ccgttgccaa aaaccagttc
     3301 agcatccagt gccagcagga cgggaagcag caccgactct tcctggcggg cggcgatttc
     3361 accaccaatg gtcgactgat tacgaacatg gcgtgagtaa acaaaaccga gggcttcacg
     3421 cagcgctgca ggaataaatc gcgcatcacg cagtggctgc aagcgagaca ttgcgccaat
     3481 ccgcagcgca ccgttatccc agtcaaccca gtccagttcc agatcctgta aggagatggc
     3541 aatcttttta tcggtacggg ttggtgtagc attgagtttg ctgcccccgg cgaaccagac
     3601 ggcttcatcc tggtagcggc gcttcagttc cagcgcctgt tcgacagagt cgggcctgaa
     3661 aaattgttca atcatcttct ttccttattt aaccctcttc cctgcgggaa gagggcatct
     3721 gtcatttatg ccagcgtatc catccgacgc cacatactgg cagcggcttt tctggcttgt
     3781 gcataaatgg aatcgcaatc gaagttaaac tgacggtctt catagaccat cacaccgttg
     3841 accatcacgc tgtgaacgct gcctgagccc ataccgaaag cgatatgccc ggcgatattg
     3901 tctgccagca gcggcgtcgg tgagttgtaa tcgcaaatgg ttaaatcagc tttgtaaccg
     3961 gcttccagaa gcccaaattt cgcgccaaag ttgcggctca tcagttcgtt accgttagtc
     4021 agggctttgg caaaactgtc aggccacagc ggaccacccg catcgcgatg tttaaagaag
     4081 gcaaatttca tctcttcaaa catgtccgaa ccaatgccgt ccgttcccaa cgccagattg
     4141 cggatgtcgc taaggtgatg gttgtagccg acatggttgt tcatgtttga acgggcgtta
     4201 tgcaccagga acgcatcgcg ctgattgagt agggtgatgt catctttcga caagtacagc
     4261 ccatgagcga ccagcgtttt gctatcgatg agatcgaatt gcgctagtcg tgccagcagg
     4321 tctttgccgt accagtggtg actgtaggaa acgtcgtaaa ggtcttccgc agcgtgaata
     4381 tgcaaaccac ggcctgtggc tttcacggct tcacgtagca tctccagacc ggcatccggc
     4441 acggtaaacg gcgcgtgagc accgatatgt gcttccacca gatacggctc ggttgctgct
     4501 ttcttcgcct catcaatctg acgggcgaag cggatgtttt cttctacacc ttcctgcaac
     4561 tctttgatgc cgttgttacg gtcagtagtt tcaaaacagg tcatcgcgcg caggccaact
     4621 tttaaaaatg cgtcgcgcaa tgtggagagc gacccgccga tatacgccgg agaggcatgg
     4681 tgatcgataa ccgatgtaca tccgctctta atcgcttcca gggaacaaat cagtccgctg
     4741 taatagagcg actcttcatc aagggcgcga tcgagccgcc accagagatt tttcagcgtt
     4801 gagatgaaat ccgggcaggg ggcgatgttt gccataattc cgcgggaaag ccccgagtaa
     4861 aaatggttgt gcgagcagac aattcccggc atcacaatcc gaccatgcat ctctttgtag
     4921 ctggcatcgg ggtagcgttg cgtcagggca tcgccgatag cgacaatcac gtcattttcg
     4981 atagcgatat caacgccttc ctgcactttc gccgggtgta actgcactgc ggtgacattc
     5041 ttcagaatca acatgattac acctccacgc ggcccagcag ataatgatga tgctggtgga
     5101 catggctgat gatgcggcac atatcgttca gctccggtgg tacgttgttg aactgacctt
     5161 cgctgtcgat gtttaacacc cagctttggt tattcagacg tactcgtacc cggcaatctt
     5221 ccacaaggaa gcctgggttg ctgctgttat caaagtcttg cgccaggctg aagacggtga
     5281 ttttgtcttt gtatggttta ccgttccacg gacagaactg agcgcagttg ccgcattcgt
     5341 tacagtaagc gtcgaggtgc agcgtctgga atctattctg gaagcctggg accgcaatgg
     5401 agacgttggc gcggttcggg cagacatcca cacacttgct gcaaacgtag ttacattcga
     5461 ggcagcgagc ggcttcctgg gcgacaaacg cgtcacgatc gtcactgttc accagagtga
     5521 tagagatatc gcctttacgt tgatagattt ccgccggatt gacgttgttc cagtatttat
     5581 cgttctggtg ggaacggata ttttcccgac taaggatggc atcggtcgcc cgacgcgcgg
     5641 ttccgacagc agcgacaatg gaggatggtc cgcgctgtac gtcgccgatc ataaagacgt
     5701 cagtcagacg agtttcgcca ttgtggtcga cgtctggcca gccgtttttg tccagcggca
     5761 cgcccatcgc attcagggct tcagtatcct gctgttcacc aatggcggtg atcaggctgt
     5821 ctacatgcag tgttactgtt tcgttggttt caaccggacg gcgacggcct ttctcatcgg
     5881 gttcgccaag cgacataacg cgcaaggtta aggtgccatc agcatcgaaa cgttccggat
     5941 tattcaggaa acggaactcc acgccgtcgt gcaacgcttc ttcatactct tcgcgccatg
     6001 cgggcatctc ttgcagtgaa cgacggtaaa cgatcgttgc tttttctacg cctggaacgc
     6061 gtaacgccgc acgagcgcag tccattgcgg tgttacccgc cccgacaacg accacatgtt
     6121 tgcccagctt gagcgctgta cccttgttgt attcacgcag gaaggggagt gatttccaaa
     6181 cattttggtt gtcacccgcc agtttcacac cgctattttt atcagtgccg gtggcaatca
     6241 gaacatagtg gaagccctga ttttttaact gttcaacggt caaatcgggt gagcagccat
     6301 actcaaattt cacgccgtga tcggcaacaa aatcgatatc gtgctgaatt aactcagccg
     6361 gaattcggaa ctgaggaatg atatttttca ccacgccgcc cgcattggct tcgcgttcaa
     6421 acagcgtaac cggatggccc gctctggcaa ggaagtaacc tgctgccaga cccgccggac
     6481 ctgcaccaat cacggcaacc ggatggcgtg aaccagaacc ggctggtttg tgccagcgtt
     6541 gcttatattc atcccaacct ttttccagcg cgactttttt cagttcgcgg atattcagcg
     6601 cactgtcgta atccaggcgg gtacagttgt attggcactg gtgatcgcaa atatgaccgg
     6661 taatggcggg cagagcgttg cgttggtata tgagttccag cgcgtcggca tagcggtgtt
     6721 cgccaagcag acggatgtat tccggaatat cttgcttgat agcgcaggca gtaacacagg
     6781 gggcaacgta gcaatcggtc agcggcaggt cttctgccac ttcaatacgc tcttctggct
     6841 tccagtgttt ctgggtgtat tccatggtta acgcatctgc tgccagtctg ttcagtcgtt
     6901 cgacgtcaac atggtcaagc ccccaggcgt cggagccttc cagctcgcgc atgcaggcac
     6961 ttaagcgcag atagccgcct ggtttcagca ggtcggttgc catagtaata gggcgaatac
     7021 ctgtatcaaa aatatcgcgg atagtcagct gactggcacc accggaataa gaaatgggca
     7081 gtttgccgtc aaaggcgcga gagagaactg ctgcaacatt gatggagagc gggaacagcg
     7141 cacggcctga catatacatc tcttcaccag gcagtgcgcc tttattgttg atggtgccga
     7201 gagtgttagt cagttttacg ccaaagccga gtgatttttc ttttgccagt gccatcaggc
     7261 gttccagcat ttccagtgct tgcgtcagct tgaggtcgtg atcaaatgac tcttctttta
     7321 agcctatgta accgaaaccg cagacatcga ggatctcacg aacacgcgcg tatcccagta
     7381 aggtcgggtt aagtttcaca aaggtgttga gccctttttc ttccagcatg tagcggcaaa
     7441 tggcttcgat ttcatgcgga ggacagccgt gcatggtgga gagggtgacg ccatgcacca
     7501 tactggtggg gatgcgagcg ggtaaggctt gcaagctttc gcgtttttcc tgcaatccgt
     7561 ggcgagctaa aaatgcgtca tcctggagta atttattcag cgtatcgcga tattgcgcga
     7621 atttcggatg gtcagatgcg tccatcatat tgtcgatgaa ctgttgcatt ggcggttgct
     7681 taataccttc gaggttgtaa ccgacgctca tattaaagat gaacgattta ccggaatcag
     7741 aaggttggaa catcgcttcg agaaggtgca gggcaaacca ggctttgagg tattcatccc
     7801 aggctttaag cagggtaaat tcggtagacc attcggtgtt aaagcactcg tcttcggcat
     7861 caatacaggg cttttccagc tccaggcggt caagaatttg gacggttttt agttcgatga
     7921 aacgtccgcc agtcagccag gaagtgacaa tattttgcgc gagctgcgtg tgcgggccag
     7981 cggcagggcc gacgggagtg gcacaggttt caccgaagac gctaacagtt ttacctttta
     8041 caggtgagta aaactgttgc tcgggaatac caaagattga gcgttgttgt tggtattcat
     8101 caaatatgcg cgtcaaaagt tcctcaaacg gaatgggacg cataatatcc cccataaccc
     8161 tctccttagc ttatacagat atcattgagt gaccggtgtt gagaaatccg gtggtttagg
     8221 gggatagagc aacaatcgca ccactcttgc caggtttggc gggaaatata aaaaaatgtg
     8281 aggcggctcg aaacttaagg gctttttgtg aatgaactca caggtgccaa atgagaatta
     8341 aaatcactcc tgaaaattat tattgccagc ccatttatca ctttttctct tttctcaaaa
     8401 tcagtgattc cttatcatta tgataaagag tgctggaggg attaaatatt tgaacgcaat
     8461 cgtttaacta cgttttttaa attttagtat accttttttg cgctgtaata aagtcgtccg
     8521 gagtatcaat atctaaaatt atttctgcat tttctatttc gacggaataa tgatctccca
     8581 taaggagtgc ctgacgcata ttggtgacat tgggtcgctg gattgcctgc atcaggcatg
     8641 gttttgacac taaaatcgga tggccaggga tgccattatg gagtggcagg attgcgccat
     8701 catttcgtaa cgaccagatt tttctgaaaa tatcgatggt gagggttggc atgtcaccgt
     8761 gggtgagaaa acaatgttct gtttgcactg cgggtactgc ggccttgact gatgttagta
     8821 aaccctgggc ataatctggg ttgtgaataa tagtgatatt gctctggttc gcataacgtt
     8881 cgtgcagttc attaccacga tagccggtga ctaaaataat tcggctacaa aactgcaacg
     8941 catttttgat acttgtatca agaattgttc cctgttgcca gggtaacatc attttccatt
     9001 gccccattct tgatgataat ccggcagcgg taattataca gtcgatggct gacatagtgg
     9061 ttccattatt tataggggct aataacgcct gataattgcg ttacttcctg atggattaag
     9121 ttggggataa tttatcccag agaggtcata aagactcgtg aaaagtatag ttgacccatc
     9181 ggcattattc attgatttag gtgcgcagaa acgtcccact gtgatttctg ttgttggtgc
     9241 tggggggaaa accagcttgc ttttttggct ggcagaactg ctccaggcta gcggcaggcg
     9301 tgtgttaatc actacgacga cacatatgtt tatgccaacg tcacactggc ccgtggtttt
     9361 ctgtcgtgat cccgccatgc ttcctcatgc gtctcttaca tcccccattt cattttgttt
     9421 tcacagctgg aaagcgaacc agggaaaagt gcagggattt acgccagaag cgattgatgc
     9481 actggtgcaa cgaccagagt gtgacgtaat tctcattgaa gcagatggct cgcgtggaat
     9541 gccgttaaaa gcccctgatg agcacgaacc ttgcataccc aaatgcagtt gctgcgtgat
     9601 tactgtgatg ggaggacata ctttgggcgc gaaagtgagc acagaaaatg tccatcgctg
     9661 gtcgcagttt gctgatatta ccgggttaac acctgatgca accttgcaac tgagcgatct
     9721 cgttgcgctg gttcgccacc ctcagggggc gtttaaaaac gtaccgcaag gttgtcggcg
     9781 agtctggttc attaaccgtt tttctcaatg tgagaatgcg attgcacaaa gcgagctcct
     9841 tcaaccgctg caacaacatg acgtagaggc aatctggctg ggcgatatac aagaacatcc
     9901 tgcaatcgcg cgcagatttg tgaattagcg gcccgggtat tccgggttac tgaaaggata
     9961 tccattgagg ttggtatgaa tattttcaca gaggctgcaa aactcgaaga gcaaaattgt
    10021 ccgtttgcga tggcgcaaat tgttgatagc cgaggctcga ctccccgcca ttctgcacaa
    10081 atgttagtgc gcgctgatgg ttctatcgtc ggtacaattg gtggcggaat ggttgagcgg
    10141 aaggtgattg aagagtcgct tcaggcattg caggaacgta agtcgcgatt attccatgga
    10201 cgtatggctc gtaacggtgc ggatgctgtc gggtcagatt gtggaggtgc aatgtcagta
    10261 tttatcagcg tccatggtat gcgtccacgt ctggtgttga ttggcgcggg gcatgtcaac
    10321 cgggcgatag cccagagtgc ggcgctatta ggatttgata ttgccgttgc cgatatttat
    10381 cgcgaaagcc tcaatcctga actattccct ccatcaacca cgcttctcca tgctgagtcg
    10441 tttggtgcag cagtggaagc actggatatt cgccctgata attttgtcct gattgccacg
    10501 aataatcagg atcgtgaagc cctcgacaaa ctcattgaac agcccattgc atggttgggg
    10561 ttgctggcaa gtcgccgcaa ggttcagctt ttcctgcgtc aattgcgtga gaaaggcgtg
    10621 gctgaagaac atattgcccg tttacatgcg cccgttggtt acaacatagg tgcggaaacg
    10681 ccgcaggaga tcgccatcag cgtgctggca gaaatattac aggtgaaaaa taacgcgccg
    10741 ggtgggctga tgatgaaacc ttctcatcct tccggacacc agctggtggt gattcgcggt
    10801 gcgggagata tcgccagtgg tgtggcgcta cgtctgtatc atgcgggttt taaagtgatc
    10861 atgctggaag tggaaaaacc gacagtgatt cgttgtaccg tggcgtttgc ccaggccgtg
    10921 ttcgatggcg aaatgacggt cgaaggcgtc actgctcgcc tggcaaccag ctctgcggaa
    10981 gcgatgaaac ttaccgaacg cggattcatc cctgtgatgg tagatcccgc ctgttcattg
    11041 cttgatgaac tgaaaccgct ttgcgtggtg gacgctattc tggcgaaaca gaatttggga
    11101 acgcgggcag atatggcacc agtaacaatc gcgcttgggc cgggctttac tgcagggaag
    11161 gattgtcatg cggtaattga aacaaatcgc gggcactggc tcggtcaggt gatttactct
    11221 ggttgtgcgc aggagaatac cggtgttcct ggcaatatta tggggcatac cacccgacgg
    11281 gtgatccgtg ctcctgctgc aggcattatg cgatccaacg tgaaattagg cgatctggtg
    11341 aaagagggcg atgtgattgc ctggattggt gagcatgaaa ttaaagcacc gttgacgggg
    11401 atggtgcgtg gcttgttgaa cgacggcctg gcagtggtcg gtggttttaa aattggtgat
    11461 atcgatcctc gtggtgaaac ggctgatttc accagcgttt ctgataaagc ccgggcgatt
    11521 ggcggcggcg tacttgaggc gttaatgatg ttgatgcatc agggcgtgaa agcgacaaaa
    11581 gaagtgctgg aagtggctta aatgaaaatg gccggatggt attactcacc cggcctgaga
    11641 ctgatgacaa acgccaaatt gcctgatgcg ctacgcttat caggcctaca agtgcattgc
    11701 aatctattga atttgcgatg ttttgtagga cggataaggc gctcgcgccg catccggcat
    11761 caacaaagcc cacttacttg acaatctttg gccggatggt attacccacc cggccttaaa
    11821 attactgaca aatcaccgtt ccggttttgc cttcaatccc ttctttcgct ttgctcaata
    11881 cggtaatcag tgcttcacga cctgcgcgag agcgagcaaa cgagacggca gcttccactt
    11941 taggcagcat agaacctttc gcaaaatgac cttcttcgat aaagcgttca gcatcactta
    12001 atgacaggcg atcgagccac tgttcattct ctttaccgaa gttaatggcc actttttcta
    12061 ccgcagtcag aatgatcagc atatcggcat caatcatttc tgctaaacgg gcgctggccc
    12121 agtctttatc gataaccgcg ctggcaccac gcagatggtt accttcacga ataacaggga
    12181 taccaccacc gccaacggtg atcaccacct ggcctgcatc taccagagct ttaaccgttt
    12241 ctttttcaat gatatcaacc ggttttggcg aggcaacaac acggcgatag ccgcgaccag
    12301 catcttcttt cagagtgtaa ccctgttttg tcagttgctc tgcttcttgc tcggtaaaga
    12361 acgaaccgat tggcttggtc gggttgagga atgccggatc gttagcgtcg acttcgacct
    12421 gagtaaccag cgttgctaca ggtttattaa tgccccgaga aagcagttct tctcttaaag
    12481 cgttttgtag atcgtaacca atataaccct ggcttaatgc aacacagaca gacatcggca
    12541 gcattggcga gtgcgcttca gtttttgcgg cagcttcaaa ggcctggtta atcatgccca
    12601 cctgcggccc gttaccatga gtgacgataa cttcatgtcc ctgggcaatt aaatcaacaa
    12661 ttgcctgaga agtaattttt accgctttca tttgcccggc cagatcatcg cccagcgcat
    12721 tcccgcccag ggcgagaaca attttcttac tcatttttcc tcactcatca ttcttgattg
    12781 tgtatagatc cctggcagtg gttagagcac gggagggaca aacggtttgc gtcgcaggaa
    12841 acgacctcgc ccggctttgc ctgtaaaggt gccgtcacag aaaatcgttt caccacggga
    12901 taaggttctg acaatcgcgc cctgacaggt aaaaccctcc catggcgagt agtcggcgtt
    12961 gtcgtggaga tggcgatgct gaatttgttg gctctgacgt gggtcgataa tcaccacgtc
    13021 gccatcggaa ccgggcgcta ataatccttt ttgcggccac aggccaaaca gcctggcggg
    13081 cattgcgctg gttaattcaa caaagcgttc cggtgttata cgtcccgtca tcacgccact
    13141 ggaaaacagt aactgcatgc ggttttccac accgggtaag ccatttgggc agcgactgaa
    13201 atcgccttta gaaatttgca ggcgttgagc catcgagaag gtgcagtgat cggtcgccac
    13261 cacgtcaatc gcaccatcgc tgatgccaca ccacagtttg tcctgctcgc gtacgttacg
    13321 cagcggtggg ctaagaatga acttcatgcc atcttctgta tcgtaactgc gttcgtccaa
    13381 caggagatat tgtgggcagg tttcaaccca gactggctgg tgattcgcac gggcaagacg
    13441 cagataatcc agacctaagc cgttagacag gtgcacgata tagagcgggg cgttaccggc
    13501 aatttgtgcc aggttaatca tgcgggcgat ggcttccgct tcgcattcca gagggcgact
    13561 caaggcatga tagcgcggcg cggttaaccc tgcggcgata aattccgccc gcttgctggc
    13621 gatagccgca tcattttccg ggtgcacggt ggtcagcgcg ccggattcat gcaagcggcg
    13681 taatgcctgc aaaacctcgt catcgttgag tttgtattga taggttaaat agagtttaaa
    13741 actgctcagt ccttcctcga ccatcatcgg gatttcgtcg aggattgcgt gattaatgtg
    13801 ctggatcaca ccgtgaaagc tgtaatcgat gaccgcttta tgggcggcat aaccacgata
    13861 aacctccagt tgatggcgta accgacagcc gtttgggcca aatcccatat ggtcaataat
    13921 ggttgttgta ccgccacacg cagctgcgcg ggtaccggta aaaaaatcat cacaactgcg
    13981 cgcgatgccg acatcaatat tgaaatgcgt atggacatcc acgccgccag ggaaaacgta
    14041 acagccagtg gcatcaattt cttcatacgg gagctgcggc gaaatattgt tgcccaactg
    14101 gcgaacaatc ccgctttcaa tcagcaaatc ctgtttggct tgtccatctg cgttaacgac
    14161 agtgccgttt ttgatcaata cgcgcatagc aaactccaaa aaccctccgg cgaaccggag
    14221 gggaataagt gaagagtaat tattcggttg ccagccagct taacgggatt gcagcgtaca
    14281 tcgcggcaca ggtcaccagg tgagatttcc aggttttttc gttaggtgca tgcgcttcag
    14341 gttctttacc agggccaaag ccgatcaccg gaatgccgtg acggcccatg atagatacgc
    14401 cgttagttga gaaggtccac ttatcaacaa ccggcgcttt gccaaacagc ccttcgtagg
    14461 cattcaccag tgctttaacg gtgaagtgat cttcttccac tttccaggtc gggaagtagc
    14521 attcggttgg gtaaaccagg ccagtccagg acggacggtc gtagttgtac atagaaacaa
    14581 ccgcgttagc tttctgtact gcaggcaggg cgcggatttc gtccagcgcg ccttcccagg
    14641 tttcgcccca ggtcagacgg cggtcaatag agacggcgca gctatctgct acagcgcaac
    14701 ggcttgggga tgtgaagaag atttcagaaa cggtgagggt gcctttgccg aggaattcgt
    14761 cataacccag acgttgggag agttcttgta attcgccaag aatcggaccc attttgaaaa
    14821 tggcgttgtc accgcgttct ggcgcagaac cgtggcagct aacaccctga acatcaatac
    14881 gaatttccat acgaccgcgc tgaccacggt atatctggca gtcggttggt tcggtactga
    14941 ccacaaattc cgggcgaatg ccggattgtt caataatgta ctgccagcac agaccgtcgc
    15001 agtcttcttc ctgcacagta ccggtaacca gcagggtata ttcatcttcc agaccgaggt
    15061 ctttaatgat tttaccggca taaaccatag atgccatgcc gccttcctgg tcggaagtac
    15121 cgcgaccacc gattagctca tcagtttcca tgccttcgta cggatcgaag tcccagtttt
    15181 tgatgttgcc aatgccgacg gtatcgatat gagcgtccat tgccaccaga cgcgggccgt
    15241 ggccgatata accgagaacg ttgcccatcg ggtcgatttc aactttatcg aagccgactt
    15301 tttccatctc ttctttaata cgatgtacta cgcgtttttc gtcgcagctt tcactgggaa
    15361 tagcaaccat gtcgcgcagg aagcgagtca tgtccgcctg gtaatctttt gctttttcaa
    15421 gaatcagttt gaatggaata ttcttagcca ttatttttct ccagattttt tactccccgt
    15481 tttgcaggga gtagttccaa tagggtgatt aaggtgctac agcgtgtttg ccttcccaga
    15541 caacttcgcg gtagtgcttc acgtcggtgt cgccttcagt actgataacc agcactacgg
    15601 catctttgtt cagcgccagt ttttccatca ggctttgacg ttgcgggtga taatgaaccg
    15661 ctgcgagaac gcccaaaccg acagcgccgg attcaccgga gatgatgcgc gggtcgttgc
    15721 cgtacggatt acccagcacg cgcataccta atgcggcaac gctgtcctgg caggagatga
    15781 attgggtggc gcagttacgt aggatttccc agcccagcgg gttaggttcg ccacaggcca
    15841 ggcccgccat gatggtggcc atatcaccgc caacgttgac gatgtcgcct ttgacgccgg
    15901 agcgataaat acagtcagct ttgtcaggtt caacaataat gctgtgcaga ttttgcgggc
    15961 tgtagacgtc gaccagataa cccagcacac caccggccat tgctccgaca ccggcttgca
    16021 gcagaacgtg cgtcggggtt acgcccattt cacgcatttg ctcgacggct tcatctgcca
    16081 gggttgcgta gccttgcatg atccaggttg ggattttggt gtaaccttcc catgccgtgt
    16141 cctgtaccac ttcccagccg tgctgctgcg cgtgttgcat ggtcaggcga acggtatcgt
    16201 catagttcat atccgtgacg atgcactcgg caccgaggtt cagaatggcg tcaacgcgtt
    16261 cctgagcaga acctttcggc atgtaaatca ccgcattctg tccgagttgc tgtgctgccc
    16321 acgccacacc gcgcccgtgg ttgccgtcgg tggtggtcgc gaaagtcatt ttttcgccga
    16381 tggcattttt caggtgctca aatgacagcg tttcgatatc aagatgatat ttttcacaca
    16441 ataattgagc gatggcgtac gcaccgccaa gcattttgaa ggcgttcaga ccgaatcgtt
    16501 ttgattcgtc tttgacgaga attttcttca caccaaaaag gtttgcgaga tcgtccagcg
    16561 cacaaagcgg tgttgggcga taaccagcta ttttctggtg gaactggcgc gccagtttgg
    16621 cctggctttg cgaaaagagc ggtgatgttt cgccgttgaa aaatttgtta tcggcgatat
    16681 caatcttcaa tgagaaaacg gacatatcct atccttttgg cctctggaaa tagagggccg
    16741 accagctgat cggcccgagg ggttatttca cgcgttcttg cgcttctttc agcagttgct
    16801 cgagcagtgc acctggtttg gcgtatttac gggacaggat catcgcggcg atgatataag
    16861 gcttccagct ggcttctttg taggtagcga tacggtattt ttcgaatacg ccttcagtca
    16921 cttccccttc tttgcaggat acgccgctga tatcagctgg caggcagtgc atgtacaggg
    16981 cttcgccatc acgggtcagt tccatcatct cttcagtaca atgccagtct ttgtgttgcg
    17041 cgttctgtgc cagacactgt ttttccagtg cttttaagcc ttcgtgatcg ttagcgcgca
    17101 gcaattcggt acgctgttcc atcactttgt aaggtgccca tgacttcgga taaacgatgt
    17161 ctgcatcttt gaacgcttct tccatgctgg tgacctgacg gaagctaccg ccagatgctt
    17221 tagcattgtt tttcgcgact tctactacat ccgggatcag gtcgtagcct tccggatggg
    17281 ccagggtgac atccataccg aagcgagtca tcagaccgat gatgccttgt ggtacagaga
    17341 gcggtttgcc atagcttgga gagtaggccc aggtcatggc gattttttta cctttcaggt
    17401 tttccagtga accaaagtgt tcacgtaacc acgcgaggtc agccattgac tgagtcgggt
    17461 ggtcaatatc gcattgcagg ttcactaaag ccggacgctg tggcagtaca ccctgcttgt
    17521 aaccgtcatc aagtgcagcg ccaacttcac gcatataggc gttgcctgcg cccagataca
    17581 tatcgtcgcg aataccaata gcgtcggcgc agaaggagat catattggcg gtttcacgca
    17641 cggtttcgcc gtgagcgatt tgtgatttgc cttcgtcgag atcttgttgt gcaaggccga
    17701 gcaggttaag cgcggaagca taagagaagc gggtacgggt ggagttgtcg cggaatacgg
    17761 aaatacctaa tccactatta aagactttgg ttgagatgtt ttcagcacgc agtgctttta
    17821 atgctgcggc aacgtccagt acttgtttca gttcatctgg cgtctgttcc cacgttaaca
    17881 aaaaatcttt ctcgtgaagg tgagaggtca gcgaattgat atccttaatc agctcattaa
    17941 cagttttcat cataaagtcc cggtagtgga atgacaggca aagtggttta tttcactgtg
    18001 acttgccaaa gtgattggtg ttttcacagc tatacctggg actaacaata agtgtgccag
    18061 aattatccgc gcggctgcaa aagactaatt tgacggataa tgtgtggggt tgatcacaaa
    18121 ttgatgccag cttctggttg tttgtaaaat acgaaaacgt ttggcttttt ttatcgcgtt
    18181 atttatttgc tgctaagtat cagaaataag ataaaggggc aggctatctc tcttcagttt
    18241 gataaactca ccgccgtaat tctcatattg atatagaaat gtgatatctc ttccataatt
    18301 tcattagaaa atgataaaaa tcttatgttt ttatcaaagc caatttcgcc tgagttaata
    18361 tcatcatgat ctgtgaacca tcaacgtctt caatgatcga cttttaagta agtctggaag
    18421 gtaaagctat ggagcttgct actacgcagt cagtattgat gcaaattcaa ccgacaattc
    18481 agcgttttgc cagaatgctt gccagcgttt tgcagcttga ggttgagatc gttgatgaaa
    18541 acttgtgtcg cgtcgccgga acgggcgcgt atgggaagtt tcttggtcgc cagttgagcg
    18601 gcaactcacg cctgctccgc cacgtcctgg aaacgaaaac tgaaaaagtt gtgacacagt
    18661 ctcgcttcga tcccctttgc gaaggttgcg atagtaaaga aaattgccgc gaaaaagcat
    18721 ttctgggtac gcctgtcatt ttacaggatc gttgtgttgg ggtgataagt ttgattgccg
    18781 ttacccacga gcaacaagag catatcagtg ataatttacg cgaattttct gattatgttc
    18841 gccatatatc caccattttt gtttcgaaac ttctggagga tcaggggcca ggagataaca
    18901 tcagtaaaat attcgcgacc atgatcgata atatggatca gggcgtatta gttgttgatg
    18961 atgaaagtcg ggttcagttt gttaatcaga ctgccttaaa aacacttggt gttgtacaaa
    19021 ataatattat tgggaaacct atccgtttca gaccattaac atttgagagt aattttactc
    19081 atggacatat gcagcatatt gtttcgtggg acgataaaag tgaattaatc attggtcaat
    19141 tgcataacat tcagggccga caattatttt taatggcatt tcaccaatcg cataccagtt
    19201 tttctgtagc aaatgcacct gatgaaccac atattgaaca attggttggc gagtgccgtg
    19261 ttatgcggca attaaaacga ctcattagcc gtattgcacc cagcccatcc agcgttatgg
    19321 tggttggtga aagcggcacg ggtaaagaag tcgtcgcccg agcaatccat aagttgagcg
    19381 gaagacggaa taaacccttt attgctatca actgtgccgc gattccggag cagcttctgg
    19441 aaagcgaact gttcggttat gttaaaggcg catttactgg cgcttctgcc aacggtaaaa
    19501 cagggttgat tcaggcggcg aatacgggca cgctgtttct cgatgaaata ggtgatatgc
    19561 cattaatgtt gcaggctaaa ttactgcgcg ctattgaggc gcgtgaaatt ctgccgattg
    19621 gtgccagtag cccaatacaa gtcgatattc gcatcatttc tgcaactaat cagaatttgg
    19681 cccagttcat tgccgaaggt aaattccgcg aagatctctt ctaccgactt aatgttatcc
    19741 cgataactct gccaccgctg cgtgaacgtc aggaagatat tgaactattg gtgcattact
    19801 ttttacatct gcatacccgt cgtctgggat cggtttatcc tggcattgct cccgatgtcg
    19861 tcgaaatatt gcgtaagcat cgttggcccg gaaacctgcg cgagttaagc aatttgatgg
    19921 aatatctggt taacgtagtt ccttcaggtg aagttatcga cagcacgcta ttgccgccaa
    19981 atctgctgaa taatggcaca acggagcaaa gtgatgtaac agaggtcagt gaggcgcacc
    20041 tgtcactcga tgatgcgggc ggcacggcgc tggaggagat ggaaaagcaa atgatccgcg
    20101 aggcgctttc acgtcataac agcaagaagc aagttgctga tgaactgggc atcggcattg
    20161 ctacgctcta tcgcaagatt aagaaatatg agttgttaaa cacataacct cagtgaatcc
    20221 ggcctgaatt caggccggat atccttttac ttcgttttct cgcaatccag aactgtattt
    20281 acaatcatct gataccccgt gcagcgacaa agatttcccg ccagtccgcg acgaatttcc
    20341 gtaatggtta atggcttctc gcgtggtttc gccagcattg ccgtggtagc cataatcagg
    20401 ccaggcgtac aaaacccgca ctgcactgcg ccggatttcg cataagcctg ctgaacatga
    20461 gaaagttttc cgcctttcgc ttcaccttcc agcgtgcgga tctcttttcc ttcagcccag
    20521 gcggcaaggt ataagcaact gtctattgct gtgccgtcga ccaacaccgt acaggcacca
    20581 cattcaccca cgcagcaccc ttgtttgaca cttagcagtc cttgttcgcg gagtaattcc
    20641 gagagcggcg tgcctggtgc ggcgtgaagc tgaaaaggca tcccgttaat ggtgcattcg
    20701 atggtaattg tttcgctgtg attcattgca attttccccc cgccgcggcg acggcttcgc
    20761 taatcacttt tttggtcatc gtctggatga gatgcagacg aaactcttta ctggcccgcc
    20821 atgaagaacg cggggcgaca tcttgcagga cagattcgct gatagcttcc agcgtttgca
    20881 ggtttaatgg cgcattttgt gcagtctgtt cggcatgttg gcagcgaatc ggcgttggcg
    20941 cggcaacacc aaatgccaga cgtaattcgc tgaaattgcc gttatccagt cggcaatgtg
    21001 cggcgcagcc aatcgttgaa atatccattg cgtcgcgcat ggcatattta aaatgcgcgc
    21061 tgcccgcgtg ttctttcggc tggggtggaa aatgaaaggc gacaaggatt tcgtcatgct
    21121 caagagacac tttgcctggc ccagtgtgaa agccattaat cgggacgaaa cgaacaccgc
    21181 gtggggagtg gatctccagt ttcgcgtcat aaatgagcgt tggcgtggca gaatctccgc
    21241 tggtggcacc gttgcaaata tttccaccgt aggtagcgac gttacggatc tgcggtccag
    21301 caatggacga ggccgcagca cataacgtcg ggagatgacg ttgagttata ggatcttcta
    21361 ttagctgggt aaatgtcgtt gcagagccga tacgtagcga gccatcttcc gccagcgtaa
    21421 ttccccgcag ctccgccaga ttatgaatat caacaatatg gcgataacgg tcattgtgat
    21481 ggtggagctg aatcagtacg tcagtgccac cggcgagcag tttggcctgc gggttgtcag
    21541 ccagcaggtt gatggcatcg gcaagggttg ctgcgcgatg gtaagaagca aaatcaaaca
    21601 tgatgttatc ctcaaatcaa tcctgccaga tgaaactctt catataaccg ttttggcgtc
    21661 agcggcagtg tattgattgc aacaccggta gccatcttca ccgcgttacg aatagcagcg
    21721 gcaacaggaa ttattggtgg ctcacccagt gacttatgtc cgtatgcgga ttgcggctca
    21781 ttgatttcga cgaacgcgct ttccagttgt ggcagatccg gcatggtcgg cattttgtaa
    21841 tccagcagat tggggttacg gaccacgccg cttttcgcat cgatgatcat ctcttcaaat
    21901 agcgcccagc caatgcccat tcccattccg ccgtgtacct gaccttctgc cagcagcgga
    21961 tttagaatat gccccgaatc atgaacgttg aggatgcggt tgatggtgac tttgcacagc
    22021 gcaatatcga ccgtcagatc aacaaaggta cagccgaacg ccggtgggtt agtggtggtt
    22081 ttgatggagc tttcagcaga gagctgcccg ccgcgttcag ggtggtagaa agcgtccatc
    22141 gccaaatctt ttaacgacat taacggctct tccggtcgtt caaccagcac gatatggcct
    22201 tttatcaggg tcatattcat cgctgactga tgtagcatga ctgcggcgtg agcgatgatt
    22261 ttctctttta ataacagtgc cgcactgcgc agcgcaggcg cggcaacata gctctggcgt
    22321 gaggcaaatg cgccgggatc gaacggcgta acgtcggtat cttgtgttga aataacgcga
    22381 acgtcgctga ccggaacccc cacggtttct gccaccattt gcgagaagac ggtgtcggca
    22441 ccctgaccga tttccgtcgc gccgctttgc acgttgatgg ttccatcctg attcatcaga
    22501 aggcgcgcgc ctgctatttc tacgccgaca ggccaggtat tagaggtgta gctaaaacag
    22561 gcgacgccaa cgccgcggcg taaattgcct tgctggttct ggcattctgc acggcgtttt
    22621 tcccattcaa agattttccg gcctttttca agacactccg gcaaccctgc gctgtaaata
    22681 cgtttgcccg tgagcggatt agcatctcct tcgcgggcgg cgttgcgtaa acgaatttca
    22741 acaggatcaa tacctaacgc tgtcgcggcg tcatcaagca tagactcaac ggcaaatacg
    22801 acttgtggcg cgccataacc acgcatcgca ccagccgagg ggaggttggt atagcaggtc
    22861 tttgaactgt aagcgtaggc acaacgagga taaaggtaag cgactttatt cccccccgca
    22921 gaagcgatgg agtgcccgtg agatgcataa gcgccggtgt tagacagaac atccagacta
    22981 taacctttca atgttccgtc gcggttcacg cccatttgcc cgtcaatagt aaaagcgtgg
    23041 cgggtacggg ttgcgaggaa acactcttca cggctaaggg aaactttcac cggaatgccg
    23101 ccaagcttgc tggtcaggaa tgccgccatt ggctcttcca gtacatcctg tttattacca
    23161 aaaccgccac cgacaaatgg tttgatgact cgtacgcatg accagggaat atccagcgcc
    23221 tgaccaacca cgcggcgaac aatgtgcggg atctgggtgc tggaaacgat ggtaattcgc
    23281 gagtcatcct ccatccacgc cagcgatgtt acgctttcca tgtgacaatg ttgaataacg
    23341 ggggtctgat agtgcccctg tacctggtag tcggcggcat cgattgtttg ttggacatta
    23401 cccgtcgaca tcgtgctttg tttcagtaaa ttgccaccgt tatggattgg tgcagcgtct
    23461 tctgccagcg ccgcttctgg cgtggtgata acgggtaatt cttgccactc aatgctgacc
    23521 aattgcgccg ctttttctgc cgtgagttca tcgcgggcca cgacgatggc aacggcgtca
    23581 ccatgatgac gaacatggcg agttagcagt gcgcgatcgg cggtatcgcg cttgttttcg
    23641 tcaagtgtcc aggcatgccc agctgtagca aatggaatat caggcacatc ttcccaggta
    23701 aaaatcgcca gtacgcccgg caaacttctg gcttgttcat cattaatact tacggcataa
    23761 ccatgtgcga tagggctacg tacatatttc gcataacaca tgcccgccat aacataatcg
    23821 tcagtatatc gtgcccgccc ggtgacctta gcaatggcat cgacgcgcat gcatgattca
    23881 cccgtagcgg ttgcttcccg cgcttccata gaaatcccct cgatttgaac atttggttct
    23941 tatggcaaag atttagtcta agcaggcaag atttacgcca gaaacgacga taagcgttag
    24001 gggaggtagg aagtgtgatg acgttaaaaa aacacagata tgatacgaaa caggactctg
    24061 aataggggca gtgatacgta tcaatatgag tggcgataaa atcaatgtga taaattggga
    24121 ttattacgca tcaggatggg aaatagggta aaaagtcagg aagttcgata cctctacagc
    24181 gaatattttg ctgatgatac ttccctttgc caaatgaaat taacctcttt ctctattacc
    24241 acgtttttcc agaagcaaga gattgggtat caagaggctg gctgctatga taaggcgtct
    24301 tgttttttaa gcgaggaaag attttgagtg cgggacgcct gaataaaaaa tctctgggta
    24361 tcgtgatgtt gttatcggtt ggactgcttt tggcgggctg ttcgggtagc aaatcatccg
    24421 atacaggaac gtattccggc tccgtttaca ccgtgaaacg gggggatacg ctatatcgta
    24481 tttcgcgcac cacgggaacc agcgtaaaag aactggcgcg actgaacggc atttcccccc
    24541 cttacaccat tgaagttggt cagaaactaa aactgggtgg ggcgaaaagt agcagtagta
    24601 cacgtaaatc aaccgccaaa tcaacgacca aaaccgcatc ggttacaccg tcatcagcgg
    24661 taccgaaatc ttcctggccg ccagtagggc aacgttgttg gttatggcca acgacaggga
    24721 aagttatcat gccgtattcg acagcagatg gcggcaataa agggattgat atctcagctc
    24781 cacggggtac acctatttac gccgcgggtg caggaaaggt ggtgtatgtg ggcaaccagc
    24841 tgcgtggcta cggtaatctc atcatgatta aacacagtga agattacatt acggcttacg
    24901 cccataatga cacgatgctg gtaaataatg ggcaaagcgt gaaggctggg caaaaaatcg
    24961 ccactatggg gagcacggat gcggcatctg ttcgcctgca tttccagatt cgttaccgtg
    25021 caacggcaat tgatccgcta cgttacttgc cgccgcaggg cagcaagcca aaatgctgat
    25081 ggcgaattaa tcagcagtca gcagcgcggc acttgctaag gagagcgtaa ggtttataat
    25141 gccttacgca tctcgaagcg ggcgtagttc aatggtagaa cgagagcttc ccaagctcta
    25201 tacgagggtt cgattccctt cgcccgctcc aatttatctt cacctcatca acctgccatt
    25261 tttgttgtat ctgattttct atattatctt aattcaatcg gttcagactt ttcgcaatcg
    25321 ccgtaacact tccccatttt tttgtaaata aaaagcgttt acgcaatgca atccttttat
    25381 aacaacccag gtaaaatacc ttattttcaa gttgttaaat aagtggttat ctacgctttt
    25441 aacaaacgat tgatggaata ttttatgaaa gggaaatctg cattaactct tctgctcgct
    25501 ggtatatttt cgtgcggcac atgccagacg acaggggcag aagtgacgag tgaatctgta
    25561 tttaacattc ttaattccac gggggcagca acagataaaa gttatttatc gttaaatcca
    25621 gataagtatc ctaattatcg tcttctgatt cactcagcca aattacaaaa tgaaataaag
    25681 agccactata ccaaagacga aattcaagga ttgctaacat taacagaaaa cacacgaaag
    25741 ttaacgttga cggagaaacc ctggggaacg tttattcttg cttctacttt cgaagatgac
    25801 aaaacagcgg cagaaaccca ctatgatgct gtctggctga gggatagttt atggggctat
    25861 atggcattag tatcagacca gggaaatagt gtagccgcaa aaaaggttct gctcaccctg
    25921 tgggattata tgtctacgcc cgaccagatt aagcggatgc aggacatcat tagcaaccca
    25981 aaacgactgg atggtattcc cgggcaaatg aatgctgtgc atatccgatt tgacagcaac
    26041 tcccccgtga tggcagatgt gcaggaagaa ggcaagccgc agctgtggaa ccataaacaa
    26101 aatgatgcgt taggtcttta tcttgatctc cttatacaag caatcaatac tggtactatt
    26161 aacgctgaag attggcaaaa aggtgaccga ttaaaatcgg ttgcgctgct gattgcttat
    26221 ctggataaag ccaatttcta tgttatggaa gattccggag cgtgggagga agatgcccga
    26281 ttaaacacat cttccgttgc actggtgacc tctggactgg aacgtttgtc aaacctactc
    26341 tcgaagaaag actctgtttt tgtctctgat ttactacgcg aagctaaagt caatgaactg
    26401 gatgaaactc tttcaacaac gaggttgaac cacctcatag ataaaggtta tgaaagaata
    26461 acgttacagc ttgatttagg gggagaatcc ccaggttatc tggaaaaaga taaacattac
    26521 agagaagccg atgcggcttt acttaatgtt atttatcctg caaatctttc aaagataaac
    26581 accagaagaa aagagcaagt tctaaaaata gttaaaaaac tagcaggtcc gtatggtata
    26641 aaaagatacg agaaggataa ttatcagtcg gcaaattttt ggttcaatga tattaaaacc
    26701 gataccgatc aaaattcgca tgtaaaaaga gaaaagagct tcattccatc aaca
//
LOCUS       DAPGOF010000057        26394 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.057, whole genome shotgun sequence.
ACCESSION   DAPGOF010000057 DAPGOF010000000
VERSION     DAPGOF010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.057"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(152..805)
                     /gene="ribB"
                     /locus_tag="RHV13_002050"
     CDS             complement(152..805)
                     /gene="ribB"
                     /locus_tag="RHV13_002050"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HDV1241582.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(909..1057)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1179..1478
                     /gene="ubiK"
                     /locus_tag="RHV13_002051"
     CDS             1179..1478
                     /gene="ubiK"
                     /locus_tag="RHV13_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HDV1241583.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELEARNNSVADLQSPPAIPPIDKAE"
     gene            complement(1513..1713)
                     /gene="glgS"
                     /locus_tag="RHV13_002052"
     CDS             complement(1513..1713)
                     /gene="glgS"
                     /locus_tag="RHV13_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HDV1241584.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQTRELELEH"
     gene            1982..2611
                     /locus_tag="RHV13_002053"
     CDS             1982..2611
                     /locus_tag="RHV13_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1449 family protein"
                     /protein_id="HDV1241585.1"
                     /translation="MILFANYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGDKVLIICRLSTTRYLAENNPWPQIL"
     gene            2638..4299
                     /locus_tag="RHV13_002054"
     CDS             2638..4299
                     /locus_tag="RHV13_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HDV1241586.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNAKIAAFEA
                     ERRREAEQTRILAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGGTAGDASAGSVSGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            4538..4597
                     /locus_tag="RHV13_002055"
     CDS             4538..4597
                     /locus_tag="RHV13_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HDV1241587.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            4914..4973
                     /gene="ibsE"
                     /locus_tag="RHV13_002056"
     CDS             4914..4973
                     /gene="ibsE"
                     /locus_tag="RHV13_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HDV1241588.1"
                     /translation="MMKLVIILVVLLLLSFPTY"
     gene            complement(5093..6526)
                     /gene="hldE"
                     /locus_tag="RHV13_002057"
     CDS             complement(5093..6526)
                     /gene="hldE"
                     /locus_tag="RHV13_002057"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HDV1241589.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGILAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(6574..9414)
                     /gene="glnE"
                     /locus_tag="RHV13_002058"
     CDS             complement(6574..9414)
                     /gene="glnE"
                     /locus_tag="RHV13_002058"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HDV1241590.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SISAHPEWLTELESQPPQADEWQHYVAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTVALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAHNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERELVRASWQKWLVEE"
     gene            complement(9437..10738)
                     /gene="ygiF"
                     /locus_tag="RHV13_002059"
     CDS             complement(9437..10738)
                     /gene="ygiF"
                     /locus_tag="RHV13_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HDV1241591.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVLPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLHHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            10980..11600
                     /locus_tag="RHV13_002060"
     CDS             10980..11600
                     /locus_tag="RHV13_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDV1241592.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            11664..12902
                     /gene="cca"
                     /locus_tag="RHV13_002061"
     CDS             11664..12902
                     /gene="cca"
                     /locus_tag="RHV13_002061"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HDV1241593.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETENALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PAKWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPE"
     gene            complement(13083..13904)
                     /gene="bacA"
                     /locus_tag="RHV13_002062"
     CDS             complement(13083..13904)
                     /gene="bacA"
                     /locus_tag="RHV13_002062"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDV1241594.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTSGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(13995..14363)
                     /gene="folB"
                     /locus_tag="RHV13_002063"
     CDS             complement(13995..14363)
                     /gene="folB"
                     /locus_tag="RHV13_002063"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HDV1241595.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            14468..15085
                     /gene="plsY"
                     /locus_tag="RHV13_002064"
     CDS             14468..15085
                     /gene="plsY"
                     /locus_tag="RHV13_002064"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDV1241596.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(15098..16030)
                     /gene="ttdR"
                     /locus_tag="RHV13_002065"
                     /gene_synonym="dan"
     CDS             complement(15098..16030)
                     /gene="ttdR"
                     /locus_tag="RHV13_002065"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HDV1241597.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVRVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            16237..17148
                     /gene="ttdA"
                     /locus_tag="RHV13_002066"
     CDS             16237..17148
                     /gene="ttdA"
                     /locus_tag="RHV13_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HDV1241598.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIVPDGDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            17145..17750
                     /gene="ttdB"
                     /locus_tag="RHV13_002067"
     CDS             17145..17750
                     /gene="ttdB"
                     /locus_tag="RHV13_002067"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HDV1241599.1"
                     /translation="MKKILTTPIKAEDLQDIHVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            17798..19261
                     /gene="ttdT"
                     /locus_tag="RHV13_002068"
     CDS             17798..19261
                     /gene="ttdT"
                     /locus_tag="RHV13_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HDV1241600.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFTVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLIPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLAITGLLWMPVVLL"
     gene            complement(19304..20317)
                     /gene="tsaD"
                     /locus_tag="RHV13_002069"
     CDS             complement(19304..20317)
                     /gene="tsaD"
                     /locus_tag="RHV13_002069"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDV1241601.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     DVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            20555..20770
                     /gene="rpsU"
                     /locus_tag="RHV13_002070"
     CDS             20555..20770
                     /gene="rpsU"
                     /locus_tag="RHV13_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDV1241602.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            20881..22626
                     /gene="dnaG"
                     /locus_tag="RHV13_002071"
     CDS             20881..22626
                     /gene="dnaG"
                     /locus_tag="RHV13_002071"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDV1241603.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            22821..24662
                     /gene="rpoD"
                     /locus_tag="RHV13_002072"
     CDS             22821..24662
                     /gene="rpoD"
                     /locus_tag="RHV13_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDV1241604.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(24741..25247)
                     /gene="mug"
                     /locus_tag="RHV13_002073"
     CDS             complement(24741..25247)
                     /gene="mug"
                     /locus_tag="RHV13_002073"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HDV1241605.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEISKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLSIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            25372..25447
                     /locus_tag="RHV13_002074"
     tRNA            25372..25447
                     /locus_tag="RHV13_002074"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25405..25407,aa:Ile,seq:cat)
     gene            complement(25500..26264)
                     /gene="nfeF"
                     /locus_tag="RHV13_002075"
     CDS             complement(25500..26264)
                     /gene="nfeF"
                     /locus_tag="RHV13_002075"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HDV1241606.1"
                     /translation="MNNTPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHEDGVASSWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFETEQFDPQRVRAAAYWHAK"
BASE COUNT         6468 a         7174 c         6670 g         6082 t
ORIGIN      
        1 tgatcataat gaaaaagaat gcctgatatt aatgaaattt tctcatagaa agagaagcgg
       61 aattaacatc ttgcatagca gggaaaaata aaaaccgaag cctgctacaa cttcggtttc
      121 ttgattaagg cagtaattta agcagcggtt ttcagctggc tttacgctca tgtgcctgac
      181 ggtatgccac caggtcttca atagtcacga gcgccatatt gtgtttattg gcaaactcaa
      241 tacactctgg tgcacgcgcc atcgtgccat cgtcattagt cagctcacac agcacaccag
      301 ccggtttaaa gcctgccagc gtcatcagat caatagtcgc ttcagtatga ccgccacgcg
      361 tcagtactcc acctgcctgc gcgcgaagtg ggaaaacgtg gcccgggcga ttcagatcag
      421 aaggttttgc gccatcggca atcgctgcgc gaacggtcgt aatacggtca gcggcagaaa
      481 caccggtagt cacaccctca gctgcttcaa tggtcacggt aaaaccggtg ccataggcgc
      541 tggtgttatt ttctaccatc attggcagat caagttgttt acggcgatct tcagtaatgc
      601 acaggcaaac aataccgcta ccgtggcgaa tggtcagcgc catctgctca acggtcatgg
      661 tttctgccgg gaagatcata tcaccttcgt tttcacggtc ttcatcatca agcaccatta
      721 caccgcgtcc ttcacgcagc gcagccagtg cattttcaac acgttcgaaa ggcgtaccaa
      781 aagaggaaag tagcgtctga ttcatggtaa aaaaacctca ctaaaattat ggttaccaga
      841 atcagggcag tcttaggagt ggcggcatat agccaaaata acgtgagcgg gtccatgccc
      901 gacagaatcg ttactctctc ccatccggac tctaaccgtc ggccccggaa ttacaccgga
      961 tctgctgtcc tttgagttcg cacccaaagc gctcgcgggc tttcaactga gttgatttac
     1021 cgccggtggg gaatttcgcc ccgccctgag aataagcgga ttcactataa cgctaatgat
     1081 tagcggcagc aacgcatagc ttcacataat tctggtttat gacttaccct tatcgcacta
     1141 caatggcact caacacctat cagtacaggg aaactacaat gattgacccg aaaaaaattg
     1201 agcaaatcgc tcgccaggtt cacgaatcaa tgcctaaagg aatcagggag ttcggggaag
     1261 atgtggagaa aaaaatccgc caaaccctac aagcgcagct gacgcgtctc gatctggtga
     1321 gccgcgaaga attcgacgta cagacgcaag tcctgttacg gactcgtgaa aaactggcgt
     1381 tactggagca gcgcatcagt gaactggagg cgcgcaataa ttcggtagca gatcttcaat
     1441 ctcctccggc tatcccgcct attgataaag cagaataaag caacacaaaa acgggccaga
     1501 cggcccgtaa tatcagtgtt ctaattcaag ttctctggtt tgcatcatct gttggcaata
     1561 ccaggcataa cgtgcgcaaa accaggttct atgttcttca tccatgttac cagtaacggc
     1621 cagaatatcg accgggcgac cttctgcgtg cattctggca aaactacgcg ccaggaaatc
     1681 gaaattattt aaagaattaa gactatgatc cataagcaca tcctccggtt atcgcatacc
     1741 ttttaaggcc atatgctttt tctgatttca gtattcccca atcagtgtag tgcctttaaa
     1801 cataacgtgc gtaaatatat tgtccccgat cacacttttt agtgaaaaat aaaaaaccat
     1861 attaaaagac tttctcttcc agaacttctc taacatgtgt tattattaac aaagacaaat
     1921 gtaagcaagg aaactatccc aaagtaaata ttgcgtcact aaatggacat tggagtgaca
     1981 tatgatttta ttcgccaact ataacacccc ttatcttttc gctatttcct ttgtactgtt
     2041 gatcggcctt ttagaaattt tagcgcttat ctgcggtcac atgctctccg gtgctcttga
     2101 tgcacatctt gaccattatg attccataac cacaggtcat attagccagg cacttcatta
     2161 tctcaatatc ggaagattgc cagctctcgt cgttctctgc ttgctggctg gtttttttgg
     2221 tcttattggc atcctgttgc agcatgcctg catcatggtc tggcaatcac cactgtcgaa
     2281 cctgttcgtg gttcctgtca gtttactgtt tacgattatt gcggtgcatt ataccggtaa
     2341 aatagttgcg ccctggattc cacgcgacca tagttcagct attacagaag aagaatatat
     2401 tggtagcatg gcattaatta ccggtcatca agccacttca ggtaatccct gcgaaggaaa
     2461 actcaccgat caatttggcc aaatacatta tttactactg gagcctgaag aaggaaaaat
     2521 tttcactaaa ggagacaaag tgttaattat ttgccgactt tctacaacgc gatatttggc
     2581 ggaaaacaat ccctggcccc aaatattgta atttattaat caaaaggaaa tttaataatg
     2641 gatgatattg ttaattctgt gccctcctgg atgtttaccg cgattattgc cgtatgcatt
     2701 ctgtttatta ttggaattat tttcgccagg ctctatcgtc gcgcttcggc agagcaagct
     2761 tttgttcgta ctggtttagg tgggcaaaaa gtggtaatga gcggtggcgc aatcgtgatg
     2821 ccgatctttc atgaaataat ccccatcaat atgaatactc tgaagctgga agtcagccgc
     2881 tcaaccattg atagcctgat tacgaaagat cgtatgcgcg tcgatgtagt agtcgctttc
     2941 tttgtgcggg taaaaccttc agtagaaggg attgccaccg ctgcccaaac gttggggcaa
     3001 cgcacgctat caccagaaga tttacgtatg ttggttgaag ataaattcgt cgatgccctc
     3061 cgtgcaacag ctgcacaaat gaccatgcat gagttacagg atacccgcga gaacttcgtg
     3121 cagggagtac aaaataccgt agctgaagat ctgtcgaaaa acggcctgga actggaaagc
     3181 gtttcactta ccaactttaa ccagacgtcg aaagaacatt tcaatccgaa caatgccttt
     3241 gacgccgaag gtttaaccaa actgactcag gagacggagc gccgtcgccg cgagcgtaac
     3301 gaagttgaac aggatgtaga agttgcggtg cgtgagaaaa accgtgatgc actttcgcgc
     3361 aagttggaga ttgaacaaca agaagcgttt atgacgcttg agcaggagca gcaggttaaa
     3421 acccgtaccg ctgagcagaa tgcgaaaatt gcggcttttg aagctgaacg tcgtcgtgaa
     3481 gcagagcaga cgcgaattct ggctgaacga cagattcagg aaacagaaat cgaccgcgaa
     3541 caggccgtcc gctcaagaaa agttgaagct gaacgtgaag ttcgcattaa agagatcgaa
     3601 cagcagcagg tcaccgaaat cgctaaccag acgaaatcga tcgctattgc cgccaaatcg
     3661 gaacaacagt cccaggcaga agcgcgtgct aaccttgcac tcgcagaagc agtaagcgcc
     3721 caacaaaacg ttgaaaccac tcgccagact gccgaagccg atcgtgccaa gcaggtggcg
     3781 ctgattgccg cagcgcagga tgcggaaacc aaagcggttg aactgaccgt gcgggcgaaa
     3841 gcagagaaag aagcggcaga gatgcaggcg gcggcgatcg ttgagttagc tgaagctacg
     3901 cgtaaaaaag gtctggcgga agcagaagcg caacgtgcgc tgaacgatgc tatcaacgta
     3961 ctttctgatg aacaaaccag ccttaaattc aaactggcat tattgcaggc gctgcctgcg
     4021 gtaattgaga aatccgttga gccgatgaag tcgatcgatg gtattaagat catacaggtc
     4081 gatggactga atcgtggtgg tactgcgggt gatgcgagcg caggtagcgt tagtggagga
     4141 aaccttgcag agcaggcatt gtctgccgcc ctttcttacc gcacacaggc accgctgatt
     4201 gactccttgc tcaatgaaat tggcgtttca ggcggctcgc tggcggcatt gacttcaccc
     4261 ttaacctcaa caactcccgt cgcagaaaac gtagaataaa ttttcaacta tgaacatccc
     4321 tggcccaaaa agccggggat tttttatatc tgcgttccgc taaaaactgc aaatgatcag
     4381 gttgttgcaa cgttttgcgc gaccgctcgt ggaaggcaaa actgccacag ggaaagcatt
     4441 gcgcgggatc cggcactcat caacaatcgg gaagtcagca aggggctgaa acgggaaagc
     4501 ccctcccgaa gaaggggcct tgtataagga aagggttatg atgaagctcg tcatcatact
     4561 gattgtgttg ttactcgtaa gtttcgcagc ttattaacag ccaatcagag gggggagaaa
     4621 tcctccctca cccttgttcc tttactttag gttgaaaaaa aatcagcgtc aagaaggttt
     4681 cattaacaca tatccctggc ccaaaaagcc ggggattttt tatatctgcg ttccgctaaa
     4741 aggtgcaaat gctcaggacg ttgcagcgtt ttgcgtgacc gctcggggaa ggcaaaactg
     4801 cttcagggaa agcgttgcgc ggggtccggc gctcatcaac aatcggggag tcagcaaggg
     4861 gctgaaacgg gaaagcccct cccgaggaag gggccttaca gaaggaaagg gttatgatga
     4921 agctcgtcat catactggtt gtgttgttac tgttaagttt cccgacttac taacaactca
     4981 tcagaggggg gagaagtcct cccttaccct tattccttta ctctaggttg aaaaaacaac
     5041 agcgtcaata ggcctgccat gtacgaagcg agatctgtga accgctttcc ggttagcctt
     5101 ttttatcctg ttggatcttc ttgataatgt tagtggtcga gcaaccgtct tcaaagttga
     5161 gcaccaacac ttcgccgccg ttggcccaga cttctttact cccggcgatc tcttccggtt
     5221 tgtagtcacc gcctttcacc agcaggtctg gcaggatccc ggcgatcaga cgctgcggcg
     5281 tgtcttcttc aaacgacact acccagtcga ccgcttccag tgcgcccagc acaatcatac
     5341 gctgttcgag tgggtttacc gggcgggaat cccctttcag ccgtttggtg gaggcatcgc
     5401 tgttgacggc aacaatcaag cggtcaccca gcttgcgggc atttgccaga taagagacgt
     5461 gcccggcatg taggatatcg aagacaccgt tggtcattac cactttttca ccacgtttac
     5521 gtgctgccgc tacagccagc ttcagttcct cttcggtcat cacgccaaag ccggtatctg
     5581 cgcgtccacg tacagcgttt tccagctcga tcggcgaaac cgtggacgtc cccagtttgc
     5641 cgaccaccac accagccgcc gcattggcaa agaagcaggc ttcttccagc gaattacccg
     5701 ctgccagcgt tgccgccagg atgccaatca ccgtgtcgcc cgcaccggta acgtcataca
     5761 cttcctgcgc ctgggttggc atatgcagcg gcgctttacc cggttgcagc agcgacatac
     5821 cctgttcgga acgggtcact aacagagccg agagttcgta atcggcaatc agtttcatgc
     5881 cgcgctcaac aatctcttct tcggtcttac atttaccgac aacagcttca aattccgaga
     5941 gattcggcgt taacagcgta gcgccgcggt agcgctcaaa atcggtacct tttggatcaa
     6001 tcagcaccgg aacacccgct ttacgcgcca gttggatcat ctgctgtacg cttgccagcg
     6061 cacctttggc gtagtcagaa agcaccagcg cgccaatcga actcagcgcc tgattaatcc
     6121 gctcgtgcag cggctgcgga tcaacacctt cgaaaccttc ttcaaaatcc agacggatca
     6181 gctgttggtt gcgggaaagt acccgtaatt tggtgatggt cggatgcgtc ggtacagaaa
     6241 cgaagtcgca tttgacgttg acgtcggcca gagatttact cagcgcgcgc gctgcatcgt
     6301 caatgcccgt caacccgacc agacgtgcat tagcaccgag agaagcgata ttcatcgcca
     6361 cgttagccgc gccgcccggg cgttcttcga tggtattcac tttaaccacg ggcaccggcg
     6421 cttccggcga gatacgactg gtggggccgt accagtaacg atccagcatc acatcaccaa
     6481 ccaccatcac tcctgcacgt tcaaactctg gcagcgttac tttcattcct gtctcctgag
     6541 agattcaaaa tttgcgcgcg ataataccat acttcattct tccaccagcc acttctgcca
     6601 gcttgcccgc accagttcac gctctgcggt gaagcaatcc tccgacacat ggcccggcaa
     6661 ttcctgtaat gccagatgat gaagttcatc gcgaagcgta gtgtaagcac gggtcagcgc
     6721 catcgcttcc tgctcttcca taatgtcgtt atgcgccagc agttccagaa tgcgcacgtt
     6781 gtctgaccag cgcgttaact tcggtttttc atgagcgtag cgcaacacca gatattgggt
     6841 aataaattcg atatcggtaa ttcccccttc atcagctttg atatcaaagc gatcgcgatg
     6901 tttattgccg agatgagcgc gcattttctc gcgcatttcc cgcacttccg tttgcagagt
     6961 tttaccttca cgcggcagcg tcataatctc gcgacgcact gcgtcaaagt gcgcggtgag
     7021 ctgcggatcg ccgtacacta cacgcgcacg caccagcgcc tgatgctccc acgtccaggc
     7081 ctcgtttttc tgataatcgg caaatgcttc tgcggatgtc accagcattc ccgccgcccc
     7141 ggacggacgc agtcgagcat ccacttcata caaaatgccg gaagaggtac gcgtactgaa
     7201 cagatgcata atgcgttgcg ccagacgcag ataaaactgc cgcccgtcga tttcccgctc
     7261 accgtcagtc atcgcatcca ttgggcaatc atggaggaag ataaggtcaa gatcggaact
     7321 gtagcctaac tcccagccgc ccagcttgcc gtagccgacc actgcaaaac cacgcccttc
     7381 gcgttcgttc aggtgattcg gcttaccgta gcgggcaacc atttgaaccc acgcctgctg
     7441 aacgacggca tctatcatgg cttccgccag ccaggttaag tgatcgctca ctttcatcac
     7501 cggtagcgta ccggcgatat ccgctgcggc gatgcgtaac agctgcgcct gtttgaactg
     7561 acgcagcgcc tcaagctgtt gctcttcgtc atcttccggc acgcgcagca aatactggcg
     7621 caactcatcg cggtaggcat cggtcgccgt cggctggtaa agggtgtttg gatcgagcaa
     7681 ttcatccagc aataatggat aacgcgccag ctggctggca atcatcggcg acgctgcaca
     7741 cagagaaatc aaatgtttaa gcgccgcggg gaattcactg agcaattcta aataggtggt
     7801 gcgggtaaca atccccacca gcaaggcggt aatgcgcgac agcgtaacgg cagcgtcttc
     7861 acgcgcacag acatcactta gcagatgcgg catcagatgg tcgagcacct gacgtcctcg
     7921 cggcccgatg gtgcgcttat ccagctcttt gcggaaatcg gcaatcagcg ttagcacctg
     7981 tttgcgatca tcctcgctaa gatgcgccag cactggcgtg gtgtcatctt cctgcaacgc
     8041 atcctgccac agctcacgcc actgttccga cagcgactct tcctgagttt cactttcatc
     8101 gtcgccaatc aattcattaa acacccggcg cacattggtc atatgtgcgg tcagcgccac
     8161 ggtcaattgc ggccagtcag caaagtccat cgcccacgcc agccgcgcac ggttaagctc
     8221 atcagaagga agcgtctggg tttgttcgtc gttaatgctt tgcagcaggt tttccagacg
     8281 ccgcaggaac agatacgcca ctcgcaattg ttcagcatcg ttttcagaaa gcagatgcag
     8341 cgcggcaatg acgctgagcg ttggcagtaa agagcgcgat tgcagcgacg gttcacgccc
     8401 accgcgaatg agctggaaca cctggacgat aaattcaatt tcgcgaatgc cacccgcgcc
     8461 aagtttgatg ttgtcggtca aaccacgtcg acgcacttca cgggcaatca tccctttcat
     8521 gttgcgcagc gactgaatca cgctgaaatc gatgtaacga cggaaaacaa acgggcgcag
     8581 catcgcacgc aactcgttag catagacgcc ttcgctatcg cccataatcc gcgccttgac
     8641 catcgcgtaa cgctcccagt cgcgcccctg ctcctggtaa taatcttcca acgcggcaaa
     8701 gctcagcacc agcgggccac tttcgccaaa cggacgcagc cgcatatcca cgcgatagac
     8761 gaagccatcc tgcgttggtt gatccagcac tttaatcagc cgctgcccca tgcgggtaaa
     8821 aaactgcgcg ttatccagtt cccggcgtcc accctgcgta caaccatgtt ccggccaggc
     8881 aaaaatcaga tcgatatcag aggagaaatt cagttccccg ccgcccagct tacccatgcc
     8941 taaaatcagc agcggttgcg cttcgccctg cgcattgcac ggcgttcccc actcgcggca
     9001 gcaggcgtca tatagccagt cacgcgccgc aacaatcagc gtctccgcca gatagctgag
     9061 ctgctgcaat atgctctctt cagtaaccag tgccagcgtt tgcgcccagg cgatacgtac
     9121 catgatgcgc cgacggaaca gccgcagctc gcgcattaac ccggcttcgt cactcacatt
     9181 actgagcgcc tcctgcaacc atgcaacgta atgctgccat tcgtcggcct gcggcggttg
     9241 gctttccagt tccgtcagcc actctggatg cgcacttatg ctgtcctgca caaaatcact
     9301 gaaagtaagt actgacttcg cctgtgcact aagtgattcc tcggctaaag gctctggcag
     9361 ccgctcaaca acggtctgcc agtactgctg taacggtgaa gagagcggct tcataagcga
     9421 ttttatcctt gcctgattaa cgttttccgc tgtgcaacca gaacggttcc tgattgtttg
     9481 cctcattacg gaaatgttca atttcgatgc gttgcccggt cgcaatagcg tgatgcagcc
     9541 cctgccagtt ctccagccag gcttgcgcga cgacaggatc atagtaaccc gccagcaaca
     9601 gtattgagtc aatatcacgc gtcaggcgtg gcaactggtc acggtagcga tcgcctaacg
     9661 gctggcagaa aacgcttttc agttcagcgg catggcggga aagatggata tcggcaaagc
     9721 gtttgaagga gtcgctgatt ttgccctgcg ctttggcatc taaaaatggc tgccatgctt
     9781 tgcttaccaa ccattcggtc aacgccagct tcgccattgc cgtttcggta gagtagaccg
     9841 ccgtcacggc agacaccgca gaagcaatgg tcgcctcgca ttgagtcagc agatcacgta
     9901 agtgagtgct cgctttacgc ggcacaatac caccgaacag catcagcgta tgacggacca
     9961 ggctaatggc tgccagcacc tgttctttcg ccgcatcgtt gccgcgtacc cacagttctt
    10021 catgatactg ccattgcgct aacgccagct cgagcgccgc ttccagcccc tgttccacat
    10081 cggcttttgc cgcaacatgc aaaatggtgg tcggtttgat ttcacgcgcc ggattgccct
    10141 gcgccagatg atagccacgc gccgctttgc tcaggctgcc ctggcgtaat ccggtttgcg
    10201 ataccagttg gttcgccagt ttcagcaccg cgcgcgtgtc gccgctaagc agttccagtt
    10261 ccagctcaca gataggttca gcaaattccc ccgctttcac ctccccctga tcgagagcga
    10321 tttcaattcg gctgccatcg accgccacca gccatttttc gcgataaaaa tcggtgctga
    10381 acagcggtag tacgcgggaa gcgagatcgg cgggcaattc gccgttcggc cagacttccg
    10441 tcggtaactg cgccaggtcg agcgtcggtt cgctcaacgc cacgttatat tccgggcgct
    10501 ggtgtaagcc gcctgtcact cttcctgcaa ctttcatggt catctcatag cgaccgtttt
    10561 cgccacgaat acgtaagccc atatcgtgcc cacgcagcca gttatccggc gtttcgtagt
    10621 aaatattcag caactgcacg gggtcatggt gctcgccgcc cagcgtattg agatggtcac
    10681 gcaacgcctc aacggcactg tgattaacaa taaactttaa ttcgatttcc tgagccatgg
    10741 cctttactta tcggttatgt cacataagtg acgatgaacg gcgaacttaa tgcgatcttt
    10801 tttgtcagta gatagtattt tgcgccaaat tgccatgcaa cgggcaattt gacgggcgta
    10861 aaagtttgaa gcagtggcaa gacctgacac agaggattcc gattgatgtc gaatgctttg
    10921 cgtagcgaca ctgatgccac tactatcgtt ccactttcca tgacaataac gacagcctga
    10981 tgccaaaatt acgcctgatc ggattaactt tactcgcact tagcgcgact gccgtctcac
    11041 acgctgaaga aacgcgctat gtttctgacg aactgaatac ctgggtccgt agcggtccgg
    11101 gagatcatta tcgcctcgtg ggcacggtta acgccggcga ggaagtgacc ttattacaaa
    11161 ctgacgccaa caccaattat gcccaggtga aagacagctc tggccgtacc gcctggatcc
    11221 cgttgaaaca acttagcact gaaccaagcc tgcgctcccg tgtgccagat ctggaaaatc
    11281 aggtcaaaac tctgaccgat aaactcacca atatcgataa cacctggaat cagcgcaccg
    11341 cagaaatgca gcaaaaagtg gcgcagagcg acagcgtgat caacgggtta aaagaagaaa
    11401 atcagaaact gaaaaacgag ctgattgtcg cgcagaaaaa ggtcgatgcc gccagcgtac
    11461 agctggatga caaacagcgc accatcatca tgcaatggtt tatgtatggc ggtggcgtgc
    11521 tggggcttgg cttgctgctc ggtctggtac tgccgcacct gatcccaagc cgcaaacgca
    11581 aagatcgctg gatgaactaa atcgccttct tcgttgcact gtcttattat taggaattgt
    11641 gtatttgacg aagggatgat ggcgtgaaga tttatctggt cggtggtgct gttcgggatg
    11701 cattgttagg gctaccggtc aaagacagag attgggtagt ggtcggcagt acgccacagg
    11761 agatgctcga cgcgggctac cagcaggtag gccgcgattt tcctgtgttt ctgcatccgc
    11821 aaacgcatga agagtatgcg ctggcacgta ccgaacggaa atccggctcc ggttacaccg
    11881 gttttacctg ctatgccgca ccggatgtca cgctggaaga tgatcttaag cgtcgcgatc
    11941 tgaccattaa tgcgctggcc caggacgata acggcgagat tatcgacccg tacaacggtc
    12001 tgggcgatct gcaaaatcgt ctgttgcgcc atgtttcccc cgcttttggc gaagatccgt
    12061 tacgcgtact gcgcgtggcg cgttttgctg cgcgttatgc ccacctcggt tttcgtattg
    12121 ccgatgaaac tctgacgctg atgcgcgaga tgacccatgc gggtgaactg gaacacctga
    12181 cgcctgaacg ggtatggaaa gagacagaaa acgccctcac cacccgcaat ccacaggtgt
    12241 tcttccaggt actgcgcgat tgcggcgcac tgcgcgtttt attcccggaa attgacgccc
    12301 tgtttggtgt tccggcccct gccaagtggc atccggaaat cgacacgggt attcatacct
    12361 taatgacgct ctcaatggcg gcgatgctga gtccgcaggt cgatgtccgt ttcgcaactt
    12421 tatgccacga tctcggtaaa ggactgacgc cgccagaact ctggccgcgt catcatggtc
    12481 atggcccggc gggtgttaag ttagtggaac aactctgcca gcgtctacgc gtgccaaatg
    12541 aaattcgcga tttagccaga ctggtggctg agtttcacga tctcatccac accttcccaa
    12601 tgctgaaccc aaaaaccatc gtcaaattat ttgattccat cgatgcctgg cgtaaaccgc
    12661 agcgtgtcga gcaactggcg ctgaccagcg aggctgacgt gcgcggcaga accggttttg
    12721 aatcagcgga ctacccgcaa ggccgttggt tgcgcgaagc ctgggaagtg gcgcagtcag
    12781 tgccgacaaa agcggtcgtt gaagcgggat ttaaaggcgt ggagattcgc gaggagctga
    12841 cccgacggcg gattgcggcg gtagccagct ggaaggaaca acgttgccca aagcctgaat
    12901 gagtattggg cattttgccg gatgcggcgt aaacgcctta tccggctaca tgtcaacgac
    12961 agttgtaggc ctgataagac gcgcaagcgt cgcatcaggc gttgattgcc ggatgcggcg
    13021 tgaacgcctt attcggccta cgcaatcgtg gacaacgcca ggcatccgac actattcctc
    13081 aatcaaaaga acacgacata caccgcagcc gccacaataa agcgataaat ggcgaacggg
    13141 ataaacgaaa tgcgcttaat caattgcagg aaggttttaa tcgctatcag cgccaccaca
    13201 aaagcggtga taaacccaac ggcaaacatc gggatatcgc cgcttgtcag gaagccccag
    13261 cttttgtaga gatcgagcgc cgttgcgccc atcatcatcg gcaccgccag caggaacgaa
    13321 aactcggaag cagcgtaacg gctcaccccc atcagcatcc cacctgaaat ggtcgccccg
    13381 gaacgggaga aacccggcca cagcgccaga cactggaaac agccaatcat aaacgcctga
    13441 cgataggtca tatcatcaag acccggcgca cgcggctctt tcggcttcag gcattcggcg
    13501 gcaatcagca acaaaccgcc aacgaccagc gcatacatca catttatcgg gttaaacaat
    13561 gacttaatcg tgtcgtggaa caacagcccc agtaccaccg ccggaatcat ccccagcaaa
    13621 atgtggatca gcgttaaacg acctttgctt tctccttcgt gctgcaacgg gcggccaaag
    13681 tggatgccaa tcaggccaaa cagacgccgc cagaacatca ctactaccgc cagaattgat
    13741 cctaactgga tcacaacttc aaaggttttc gccgtgtcgc cctcaaaccc caacaagtga
    13801 ccgacaataa tcatatggcc cgtgctggat accggcagaa attctgtcaa tccttcgacc
    13861 acacccaata ttgccgctat cagcagcgag tgcatatcgc tcatcaataa acccctaaat
    13921 tattaaaatg taccgcttgt ccgaactact gcagtatgac caggttataa ccgtttggtt
    13981 taacagctgt aaaattaatt attttctttc agattattgc cacgctcaat gattacgcca
    14041 acattcgccg cccgcgccac tgcgcctggc ttgctgagtt tgatacgcac ccacggcgag
    14101 ttgaagcgtg ctaacagcag ctccgccacc tcttccgcca cgcgctccac cagcgcaaaa
    14161 cgtgccccct cgacgtggct gaccaccgtt tctgcaatgt cagcgtagct gaggcaatcc
    14221 gccacatcat cacttttcgc cgctttacgg ttatcccacg ccatttcgat atcgaacact
    14281 aacttctgtt cgatggtctg ttcccagtcg taaacaccaa tagtggtgat taccgaaagt
    14341 tgctctataa atacaatatc catcacgtcc tgcctgcttt ttggctaacc ggataccact
    14401 tccggcgaaa tgtgcgtatt atccacagat tcatcgttga acacgaattt tcaaaacgga
    14461 acagcttatg agtgcaatcg cgcctggaat gatcctcatc gcgtacctct gcggctccat
    14521 ttccagtgcc attctggttt gccgcttgtg tgggctgccc gatccgcgaa ccagcggctc
    14581 cggcaatcca ggcgcaacca atgtgttacg tatcggtggc aagggagcag ccgtagcagt
    14641 actgattttc gacgttctga aaggaatgtt gcctgtctgg ggcgcgtatg aattaggtgt
    14701 cagccccttc tggctaggct taattgccat cgccgcctgt cttggacaca tctggcccgt
    14761 tttcttcgga tttaaaggag gaaaaggcgt tgctaccgct tttggagcaa tcgcaccaat
    14821 tggctgggat ctcaccggag taatggcggg aacctggtta ctgaccgtgc tattgagcgg
    14881 atactcgtcg ctgggagcga ttgtcagtgc actgattgct ccgttttatg tctggtggtt
    14941 taagccacaa ttcaccttcc cggtttcgat gctctcttgc ctgatcctgc tgcgtcatca
    15001 tgacaacatc cagcgtctgt ggcgtcgtca ggagactaaa atctggacga aattcaaaag
    15061 aaagcgcgaa aaggatcccg agtgatttct ggtggatcta catgacctga tagccttcat
    15121 cgggcttgcc cagccgttgc tggcaccatg ccgccagaaa ttccacgcag acacgtaatt
    15181 tcatgctgcg atagagcggc tcccggtaaa cagcccagat attggcgctt tgtgcatact
    15241 ccggcaatac tcgcaccagt ttgccactct ccagaaacgg caacacatcc cactcggaac
    15301 gcagcataat ccctttgccc tccagcgccc attgcagcac aatctcgccg ctattggagg
    15361 aaagatgccc gctaaccttc accgattttt tctcctgacc gttccccaac tcccatattc
    15421 cgtgggtcat atcgcgctct ttggtcacca gacagtcatg acgacttaat tcctgtaggg
    15481 attgtggctg cggatatttt tgcagatact caggtgctgc gcataatatt cttttatttt
    15541 tcgttaacag atgcgcaata taataatcag gaatttcgtc attaatacga atatccagat
    15601 caatattatc ctgcactaaa tcaatttgtc gatcgaacaa ttcaaaatgc acctgtaact
    15661 caggataatt gcgcatcagt tcggtaatgg ctggcgcaat atggctgcgc ccaaaaccaa
    15721 aactgcaacc aatacgaatc atcccctccg ggcgcgtttt gatttgcgtg acgtcatcga
    15781 ccagtcgctg atattgggtg aggatttcca gcgcatgttc gtaacaacgt tggccacttt
    15841 cagtcagcgc cacgccgcga gcggagcggt tcagcagcgt tgttgccagc gtattttcaa
    15901 ggatttggat ccgcttggtg acaaatgcag gcgtttgccc aagcgtcgcc gctgcagcac
    15961 taaaactccc gctgtggacg atttctacca gtacctgcaa gtctttggct aaaggccagc
    16021 tattcagcat cgttaattat ccgcagttgt gataagcgca gtgtatttcg caaaacattg
    16081 ccattccctg ctctggcgca atattcattc ttattttcgc gttgattcac gaactgttaa
    16141 ttacatattc acggtggcaa aaaatataaa accacatttt tagtggtagt ttgtggcggt
    16201 gaatttttcc agacaaatac aaaaactgga gttgccatga tgagcgaaag taataagcaa
    16261 caggcagtga ataagttgac ggagattgtc gctaacttta ccgccatgat ttctacccga
    16321 atgcctgatg acgtggtgga taaactaaaa cagctaaagg atgccgaaac gtcgtcgatg
    16381 gggaaaatca tctaccatac gatgttcgac aacatgcaaa aagcgattga cctgaatcgt
    16441 cctgcctgtc aggacaccgg ggagattatg ttcttcgtta aagtcggttc ccgcttccca
    16501 ctgcttggcg agctgcaaag catactcaaa caagccgtgg aagaggcaac cgtcaaagcg
    16561 ccactacgtc acaatgcggt agaaattttt gacgaagtga acacaggcaa aaataccggc
    16621 agcggcgtac cgtgggtcac ctgggatatc gtccccgacg gtgacgatgc ggaaatcgaa
    16681 gtttacatgg caggcggcgg ctgcacgcta ccaggccgct cgaaagtgtt aatgccgtca
    16741 gaaggctacg aaggcgtggt gaaattcgtc ttcgaaaata tctccaccct cgccgtaaac
    16801 gcctgtccac cggtactggt gggcgtgggc atcgccacct cggtggaaac cgccgccgta
    16861 ctctcgcgta aagccatttt gcgcccgatt ggcagccgcc accccaatcc aaaagcggca
    16921 gagctggagc tacgcctgga agaaggactc aaccgtctgg ggattggtcc acaagggctg
    16981 actggcaaca gttcagtgat gggcgtgcat atcgaatctg ccgcccgcca tccgtcaacc
    17041 atcggcgttg ctgtttctac cggctgctgg gcgcatcgtc gcggcacact gctggttcat
    17101 gccgatctca cctttgaaaa tctgtctcac acccggagcg cgttatgaaa aagatcctga
    17161 caaccccgat caaagctgaa gatctgcaag atattcacgt cggcgatgtg atctatctga
    17221 ccggtacgct ggtgacctgc cgtgacgttt gtcaccgccg attgatcgaa ctgaaacgtc
    17281 cgatccctta cgatctcaac ggcaaagcga ttttccacgc tggccccatc gtgcgcaaaa
    17341 acggcgacaa atgggagatg gtctccgtcg gtccgacaac cagtatgcgt atggaaagtt
    17401 ttgaacgtga atttatcgaa cagaccggcg tgaaactggt ggtcggcaaa ggcggaatgg
    17461 ggccgctgac cgaagaaggc tgtcagaaat tcaaggcgct acatgtgatt ttcccggcag
    17521 gctgcgcggt gctggcggca acccaggtag aagagattga agaagtgcac tggacagaac
    17581 tcgggatgcc ggagtcactg tgggtctgcc gggtcaaaga gttcggcccg ctgattgtct
    17641 ctattgatac ccacggcaac aacctgatag ccgaaaacaa aaagctgttc gccgaacgcc
    17701 gcgatcccat cgtggaagag atctgcgagc acgtccatta catcaaataa ccctcccgga
    17761 gaggctcgcc cctctccttt ttcgcaggca taacacgatg aaaccttcca ctgaatggtg
    17821 gcgatacctt gcgccgctgg cggtcatcgc cattattgct ctaattccgg ttcccgcagg
    17881 gctggagagt catacctggc tctactttac cgtttttact ggcgtgatcg ttggactgat
    17941 cctcgaaccc gtgccgggtg ccgtggtggc gatggtgggt atctccatca tcgccatact
    18001 ctctccctgg ctgctgttca gccccgaaca gctcgcccag ccaggcttta aattcactgc
    18061 aaaatccctc tcgtgggcgg tttccggttt ttctaattcg gttatctggc tgattttcgc
    18121 cgcctttatg tttggcacag gctatgaaaa aaccgggctg gggcgacgta tcgcgctgat
    18181 tctggtgaaa aagatggggc atcgcacgct gtttcttggc tatgcggtga tgttctccga
    18241 gctgatcctc gcacctgtca caccgtccaa ctcggcgcgt ggtgcgggga ttatctatcc
    18301 catcatccgt aacctgccgc cgctctatca atcacaacca aacgacagca gttcgcgcag
    18361 tattggctcg tacatcatgt ggatggggat tgttgccgac tgcgtgacca gcgccatttt
    18421 cctgacggcg atggctccta acttgctgtt aattggactg atgaaaagcg catctcacgc
    18481 cacgctgagt tggggcgact ggttcctcgg gatgttgccg ctcagtattt tactggtcct
    18541 gctgattccc tggctggctt acgtgttgta cccgcctgta ctgaagtcag gcgatcaggt
    18601 gccgcgctgg gcagagacgg aactgcaggc aatgggcccg ctctgttcgc gtgaaaaacg
    18661 gatgctgggg ctgatggtag gcgcgctggt gctgtggatt ttcggcggtg attatatcga
    18721 tgccgcgatg gtcggttaca gcgtggtggc gctgatgctg cttctgcgca ttatcagttg
    18781 ggacgacatt gtcagtaata aagcggcgtg gaacgttttc ttctggctgg cctcgcttat
    18841 caccctcgcg accggactca acaacaccgg ttttattagc tggtttggca aactgttagc
    18901 aggcagctta agcggttatt cgccgacgat ggtgatggtg gcgttgattg tggtgtttta
    18961 tctgctgcgc tacttttttg ccagcgccac ggcgtatacc tccgctctcg caccgatgat
    19021 gattgccgct gcgctggcga tgccggaaat cccgctgccg gtgttctgcc tgatggttgg
    19081 tgcggcaatt ggtctgggga gcattcttac accatacgcc accggcccca gtccgattta
    19141 ctacggcagt ggttatctgc caacggcgga ttactggcga ctgggggcga tttttgggct
    19201 gatattcctc gtattgctgg cgattaccgg cttactgtgg atgcccgtgg tgttgcttta
    19261 agtaaggacg gtgccggaag taactctccg gcacccgata gttttacgca gccggtaact
    19321 ccgccagcgg ccagcgcgga cgcacgctaa cgccgagatc cgccgtcgcg cctgctttaa
    19381 accgcaccat tccggcatag gcgatcatcg cgccgttatc agtacaaaat tccggacgcg
    19441 cgtagaacac ttcgccgcgg cgttttttca tcatttcagc cagcttcgcc cgtaacgtgc
    19501 ggttagcact cacgccgccc gccatgacca gtcgcttaaa gcccgtctga tccagcgcac
    19561 gcttacactt aatcatcaac gtatcgacca ccgcatcttc aaaggcgcgg gcgatatcag
    19621 cacgcgtctg gtcgtcggtg ccgttgtcac gaatggtatt tgccgcgaag gttttcaggc
    19681 cggagaagct gaaatccagc cccggacggt cggtcatcgg acgcgggaag acaaagcgcc
    19741 cggcagtacc ctgagccgcc attttcgaca gtaacggccc gccaggataa tccagcccca
    19801 gcagcttcgc ggttttatca aacgcttcac cggcggcatc atcgatagac tcgccgagca
    19861 gctcgtactg accaatgcca gtcacgctga ttaactgcgt atgaccgccg gaaaccagca
    19921 gcgcgacaaa cggaaattcc ggggggttat cttccagcat cggcgctaac agatgccctt
    19981 ccatatggtg tacagggatc gccggaacgt cccaggcaaa cgccagagaa cgccccacgg
    20041 tcgcgccaac cagtagcgcg ccgactaatc cagggcctgc ggtataggcc acagcatcaa
    20101 tgtcttttgc cgttaaacca gactccttta gcgccgcctg gatcaacggt acggttttac
    20161 gcacatgatc gcgggaggcc agctcaggca caacgccccc gtagtcagcg tgcaatttca
    20221 cctgactata caattggttg gctaacaaac ctttttcatc gtcgtaaatg gcgatgccgg
    20281 tttcatcgca ggaagtttca atacccagta cacgcatgac ttgttttacc tcgctttatt
    20341 accgcgcagt gtaggaccaa tgcgggttga tgtaaaactt tgttcgcccc tggagaaagc
    20401 ctcgtgtata ctcctcaccc ttataaaagt ccctttcaaa aaaggccgcg gtgctttaca
    20461 aagcagcagc aattgcagta aaattccgca ccattttgaa ataagctggc gttgatgcca
    20521 gcggcaaacc gaattaatca aaggtgagag gcacatgccg gtaattaaag tacgtgaaaa
    20581 cgagccgttc gacgtagctc tgcgtcgctt caagcgttcc tgcgaaaaag caggtgttct
    20641 ggcggaagtt cgtcgtcgtg agttctatga aaaaccgact accgaacgta agcgcgctaa
    20701 agcttctgca gtgaaacgtc acgcgaagaa actggctcgc gaaaacgcac gccgcactcg
    20761 tctgtactaa ttccccgaga gcgttgctct ccgatcagac cgagttgtag ttgtaaggcc
    20821 gtgcttccga aaggaatgcg cggcttattt tcgtttatga attgctaaaa atcggggcct
    20881 atggctggac gaatcccacg cgtattcatt aatgatctgc tggcacgcac tgacatcgtc
    20941 gatctgatcg atgcccgtgt gaagctgaaa aagcagggca agaatttcca cgcgtgttgt
    21001 ccattccaca acgagaaaac cccgtccttc accgttaacg gtgagaaaca gttttaccac
    21061 tgctttggat gtggcgcgca cggcaacgcg atcgacttcc tgatgaacta cgacaagctc
    21121 gagttcgtcg aaacggtcga agagctggcg gcaatgcaca atcttgaagt gccatttgaa
    21181 gcaggtagcg gccccagcca gatcgagcgc catcagcggc aaacgcttta tcagttgatg
    21241 gacggtctga atacgtttta ccaacaatct ttacagcaac ctgttgccac gtctgcgcgc
    21301 cagtatctgg aaaaacgcgg attaagccac gaggttatcg cccgctttgc gattggtttt
    21361 gcgccccccg gctgggacaa cgtcctgaag aggtttggcg gcaatccaga aaatcgccag
    21421 tcattgattg atgcgggcat gttggtcact aacgatcagg ggcgcagtta cgatcgtttc
    21481 cgcgagcggg tgatgttccc cattcgcgat aaacgcggtc gggtgattgg ttttggcggg
    21541 cgtgtgctgg gcaacgatac ccccaaatac ctgaactcgc cggaaacaga cattttccat
    21601 aaaggtcgcc agctttacgg tctttatgaa gcgcagcagg ataacgctga acccaatcgt
    21661 ctgcttgtgg tcgaaggcta tatggacgtg gtggcgctgg cgcaatacgg cattaattac
    21721 gccgttgcgt cgttaggtac gtcaaccacc gccgatcaca tacaactgtt gttccgcgcg
    21781 accaacaatg tcatttgctg ttatgacggc gaccgtgcag gccgtgatgc cgcatggcga
    21841 gcgctggaaa cggcgctgcc ttacatgaca gacggccgtc agctacgctt tatgtttttg
    21901 cctgatggcg aagaccctga cacgctggta cgaaaagaag gtaaagaagc gtttgaggcg
    21961 cggatggagc aggcgatgcc gctttccgca tttctgttta acagcctgat gccgcaagtt
    22021 gatctgagta cccctgacgg gcgcgcacgt ttgagtacgc tggcactgcc attgatatcg
    22081 caagtgccgg gcgaaacgct gcgaatatat cttcgtcagg aattaggcaa caaattaggc
    22141 atacttgatg acagccagct tgaacgatta atgccaaaag cggcagagag cggcgtttct
    22201 cgccctgttc cgcagctaaa acgcacgacc atgcgtatac ttatagggtt gctggtgcaa
    22261 aatccagaat tagcgacgtt ggtcccgccg cttgagaatc tggatgaaaa taagctccct
    22321 ggacttggct tattcagaga actggtcaac acttgtctct cccagccagg tctgaccacc
    22381 gggcaacttt tagagcacta tcgtggtaca aataatgctg ccacccttga aaaactgtcg
    22441 atgtgggacg atatagcaga taagaatatt gctgagcaaa ccttcaccga ctcactcaac
    22501 catatgtttg attcgctgct tgaactgcgc caggaagagt taatcgctcg tgagcgcacg
    22561 catggtttaa gcaacgaaga acgcctggag ctctggacat taaaccagga gctggcgaaa
    22621 aagtgattta acggcttaag tgccgaagag cgatcgggaa gcccccgaca gccgcactga
    22681 gaggcagcgg caaatatata agtacgccct cgtaattatc gttggcggta aacaaccgtt
    22741 ggatttcagc gttaacggct gaaggacatc gggtcaatcg cccaacacca acctcatgaa
    22801 ataagtgtgg ataccgtctt atggagcaaa acccgcagtc acagctgaaa cttcttgtca
    22861 cccgtggtaa ggagcaaggc tatctgacct atgccgaggt caatgaccat ctgccggaag
    22921 atatcgtcga ttcagatcag atcgaagaca tcatccaaat gatcaacgac atgggcattc
    22981 aggtgatgga agaagcaccg gatgccgatg atctgatgct ggctgaaaac accgcggacg
    23041 aagatgctgc cgaagccgcc gcgcaggtgc tttccagcgt ggaatctgaa atcgggcgca
    23101 cgactgaccc ggtacgcatg tacatgcgtg aaatgggcac cgttgaactg ttgacccgcg
    23161 aaggcgaaat tgacatcgct aagcgtattg aagacgggat caaccaggtt caatgctccg
    23221 ttgctgaata tccggaagcg atcacctatc tgctggaaca gtacgatcgt gttgaagcag
    23281 aagaagcgcg tctgtccgat ctgatcaccg gctttgttga cccgaacgca gaagaagatc
    23341 tggcacctac cgccactcac gtcggttctg agctttccca ggaagatctg gacgatgacg
    23401 aagatgaaga cgaagaagat ggcgatgacg acagcgccga tgatgacaac agcatcgacc
    23461 cggaactggc tcgcgaaaaa tttgcggaac tacgcgctca gtacgttgta acgcgtgaca
    23521 ccatcaaagc gaaaggtcgc agtcacgctg ccgctcagga agagatcctg aaactgtctg
    23581 aagtattcaa acagttccgc ctggtgccga agcagtttga ctacctggtc aacagcatgc
    23641 gcgtcatgat ggaccgcgtt cgtacgcaag aacgtctgat catgaagctc tgcgttgagc
    23701 agtgcaaaat gccgaagaaa aacttcatta ccctgtttac cggcaacgaa accagcgata
    23761 cctggttcaa cgcggcaatt gcgatgaaca agccgtggtc ggaaaaactg cacgatgtct
    23821 ctgaagaagt gcatcgcgcc ctgcagaaac tgcagcagat tgaagaagaa accggcctga
    23881 ccatcgagca ggttaaagat atcaaccgtc gtatgtccat cggtgaagcg aaagcccgcc
    23941 gtgcgaagaa agagatggtt gaagcgaact tacgtctggt tatttctatc gccaagaaat
    24001 acaccaaccg tggcttgcag ttccttgacc tgattcagga aggcaacatc ggtctgatga
    24061 aagcggttga taaattcgaa taccgccgtg gttacaagtt ctccacctac gcaacctggt
    24121 ggatccgtca ggcgatcacc cgctctatcg cggatcaggc gcgcaccatc cgtattccgg
    24181 tgcatatgat tgagactatc aacaaactca accgtatttc tcgccagatg ctgcaagaga
    24241 tgggccgtga accgacgccg gaagaactgg ctgaacgtat gctgatgccg gaagacaaga
    24301 tccgaaaagt gctgaagatc gccaaagagc caatctccat ggaaacgccg atcggtgatg
    24361 atgaagattc gcatctgggg gatttcatcg aggataccac cctcgagctg ccgctggatt
    24421 ctgcgaccac cgaaagcctg cgtgcggcaa cacacgacgt gctggctggc ctgaccgcgc
    24481 gtgaagcgaa agttctgcgt atgcgtttcg gtatcgatat gaacaccgat cacacgctgg
    24541 aagaagtggg taaacagttc gacgttaccc gtgaacgtat ccgtcagatc gaagcgaagg
    24601 cgctgcgcaa actgcgtcac ccgagccgtt ctgaagtgct gcgtagcttc ctggacgatt
    24661 aatcggtagg ccggatcagg cgttacgccg cacccggcac ctgtcccttt gcacaaacgc
    24721 caccttttcg gtggcgtttt ttatcgccca cgcaccacca gcgcctggtc cagctcacga
    24781 tacgcttcaa ccagtttctc cagtgaaacg cgacttaaac cgctgggatt tggcagcacc
    24841 caaatctgcg tcgaaccaat gctgagcgtt tgtttccccc actgtgcacc gcgctggctg
    24901 aatccctgtt catatgcttg tttgcccaga atcgccaacg cttgcggctg ataatcttca
    24961 attttttcaa tcagcttacg cccacctgcg tgtagctctt gctttgaaat ttcattggct
    25021 tgcaccgttg gcctgtctac cagtttggtg acgccacaac gataatccag cagatgctgt
    25081 gcctcctgcg gcttcaactg acggtcggta aacccggcct gatatatcac cttccagaag
    25141 cgatttgccg gatgagcaaa gggaaaacca gtcccggcgg atgaaagccc agggttgata
    25201 ccgcaaaaca cgacccgtaa ccctggagcc aaaatatcct caaccatatt tactcctttc
    25261 taacatcacc aggaaaagta taacggattg aaaatacatt gtttataaaa acagcaggcg
    25321 cgcggtaatg gctggattgc gacacggagt tactttataa tccgctacca tggcccctta
    25381 gctcagtggt tagagcaggc gactcataat cgcttggtcg ctggttcaag tccagcaggg
    25441 gccaccagat atagcaaagg ctgacgagaa atcgtcagcc tttttctttt acatatcagt
    25501 tactttgcgt gccagtaggc cgctgcacga acccgctgtg ggtcaaactg ttccgtttca
    25561 aagcggcggc ttaaattctt aacgactttg ccttcgccgg ttatccagat gaagtaatca
    25621 tcggcaggga tttgcatctg cgccagacgc gcatctaccg cctgctcatc atgtgccagc
    25681 cattcgatat taaaaccatc aaggtgcgcg agataatcct gacaggcgtt atcccgcacg
    25741 ctaaccagcg cgctaacttg cggtttaacg gcaagtttgc tcaacgtttc caggcggcgg
    25801 cgcagtgcgg gcattccgga ttcatcgcag acatacagct gatacgcgta atcttccggc
    25861 accaccagcg aaccgcgcgg acctgccacc gtgagtttat cgcccggttg ggcctgcatt
    25921 gcccagctac tggcgacgcc gtcttcgtga ataaagaaat caatcgccag ttcatggcgt
    25981 agttcgtcat acaacggcgt atagtcacgc gacggtgggc gtggtccttc cggccagacg
    26041 atgccctctt ccgttaccgt tggcggcaca aagtgagcgt caggttgagg aaagaagagt
    26101 ttgctgtgat cgtcaaagcc gcgcgatgta aaaccgtcca gcgcctcgcc gccaaggaca
    26161 atgcgctgaa aaccggcgct gatacgctca acgcgtagca cagtcagttc acggaagcgc
    26221 agatcattgc gaacgcgctg cgggtagcgg ggggtgttat tcattgttat cgccttcgtg
    26281 atggtaatca gatatatcta aataaaactc gcaaatgata atgattgtta atcatgataa
    26341 atgcaagcga tttgtagaac tgatatgtct atagtctgat aagacgaacc gcct
//
LOCUS       DAPGOF010000058        26119 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.058, whole genome shotgun sequence.
ACCESSION   DAPGOF010000058 DAPGOF010000000
VERSION     DAPGOF010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26119
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.058"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            198..392
                     /gene="bfd"
                     /locus_tag="RHV13_002076"
     CDS             198..392
                     /gene="bfd"
                     /locus_tag="RHV13_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HDV1241607.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            465..941
                     /gene="bfr"
                     /locus_tag="RHV13_002077"
     CDS             465..941
                     /gene="bfr"
                     /locus_tag="RHV13_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HDV1241608.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGVPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(938..1405)
                     /locus_tag="RHV13_002078"
     CDS             complement(938..1405)
                     /locus_tag="RHV13_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDV1241609.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            1784..2095
                     /gene="rpsJ"
                     /locus_tag="RHV13_002079"
     CDS             1784..2095
                     /gene="rpsJ"
                     /locus_tag="RHV13_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDV1241610.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2128..2757
                     /gene="rplC"
                     /locus_tag="RHV13_002080"
     CDS             2128..2757
                     /gene="rplC"
                     /locus_tag="RHV13_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDV1241611.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2768..3373
                     /gene="rplD"
                     /locus_tag="RHV13_002081"
     CDS             2768..3373
                     /gene="rplD"
                     /locus_tag="RHV13_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDV1241612.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3370..3672
                     /gene="rplW"
                     /locus_tag="RHV13_002082"
     CDS             3370..3672
                     /gene="rplW"
                     /locus_tag="RHV13_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDV1241613.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3690..4511
                     /gene="rplB"
                     /locus_tag="RHV13_002083"
     CDS             3690..4511
                     /gene="rplB"
                     /locus_tag="RHV13_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDV1241614.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4528..4806
                     /gene="rpsS"
                     /locus_tag="RHV13_002084"
     CDS             4528..4806
                     /gene="rpsS"
                     /locus_tag="RHV13_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDV1241615.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4821..5153
                     /gene="rplV"
                     /locus_tag="RHV13_002085"
     CDS             4821..5153
                     /gene="rplV"
                     /locus_tag="RHV13_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDV1241616.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5171..5872
                     /gene="rpsC"
                     /locus_tag="RHV13_002086"
     CDS             5171..5872
                     /gene="rpsC"
                     /locus_tag="RHV13_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDV1241617.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5885..6295
                     /gene="rplP"
                     /locus_tag="RHV13_002087"
     CDS             5885..6295
                     /gene="rplP"
                     /locus_tag="RHV13_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDV1241618.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6295..6486
                     /gene="rpmC"
                     /locus_tag="RHV13_002088"
     CDS             6295..6486
                     /gene="rpmC"
                     /locus_tag="RHV13_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDV1241619.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6486..6740
                     /gene="rpsQ"
                     /locus_tag="RHV13_002089"
     CDS             6486..6740
                     /gene="rpsQ"
                     /locus_tag="RHV13_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDV1241620.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6905..7276
                     /gene="rplN"
                     /locus_tag="RHV13_002090"
     CDS             6905..7276
                     /gene="rplN"
                     /locus_tag="RHV13_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDV1241621.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7287..7601
                     /gene="rplX"
                     /locus_tag="RHV13_002091"
     CDS             7287..7601
                     /gene="rplX"
                     /locus_tag="RHV13_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDV1241622.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7616..8155
                     /gene="rplE"
                     /locus_tag="RHV13_002092"
     CDS             7616..8155
                     /gene="rplE"
                     /locus_tag="RHV13_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDV1241623.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8170..8475
                     /gene="rpsN"
                     /locus_tag="RHV13_002093"
     CDS             8170..8475
                     /gene="rpsN"
                     /locus_tag="RHV13_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDV1241624.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8509..8901
                     /gene="rpsH"
                     /locus_tag="RHV13_002094"
     CDS             8509..8901
                     /gene="rpsH"
                     /locus_tag="RHV13_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDV1241625.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8914..9447
                     /gene="rplF"
                     /locus_tag="RHV13_002095"
     CDS             8914..9447
                     /gene="rplF"
                     /locus_tag="RHV13_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDV1241626.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9457..9810
                     /gene="rplR"
                     /locus_tag="RHV13_002096"
     CDS             9457..9810
                     /gene="rplR"
                     /locus_tag="RHV13_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDV1241627.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9825..10328
                     /gene="rpsE"
                     /locus_tag="RHV13_002097"
     CDS             9825..10328
                     /gene="rpsE"
                     /locus_tag="RHV13_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDV1241628.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10332..10511
                     /gene="rpmD"
                     /locus_tag="RHV13_002098"
     CDS             10332..10511
                     /gene="rpmD"
                     /locus_tag="RHV13_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDV1241629.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10515..10949
                     /gene="rplO"
                     /locus_tag="RHV13_002099"
     CDS             10515..10949
                     /gene="rplO"
                     /locus_tag="RHV13_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDV1241630.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10957..12288
                     /gene="secY"
                     /locus_tag="RHV13_002100"
     CDS             10957..12288
                     /gene="secY"
                     /locus_tag="RHV13_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDV1241631.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12320..12436
                     /gene="rpmJ"
                     /locus_tag="RHV13_002101"
     CDS             12320..12436
                     /gene="rpmJ"
                     /locus_tag="RHV13_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDV1241632.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12583..12939
                     /gene="rpsM"
                     /locus_tag="RHV13_002102"
     CDS             12583..12939
                     /gene="rpsM"
                     /locus_tag="RHV13_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDV1241633.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12956..13345
                     /gene="rpsK"
                     /locus_tag="RHV13_002103"
     CDS             12956..13345
                     /gene="rpsK"
                     /locus_tag="RHV13_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDV1241634.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13379..13999
                     /gene="rpsD"
                     /locus_tag="RHV13_002104"
     CDS             13379..13999
                     /gene="rpsD"
                     /locus_tag="RHV13_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDV1241635.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14025..15014
                     /gene="rpoA"
                     /locus_tag="RHV13_002105"
     CDS             14025..15014
                     /gene="rpoA"
                     /locus_tag="RHV13_002105"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDV1241636.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15055..15438
                     /gene="rplQ"
                     /locus_tag="RHV13_002106"
     CDS             15055..15438
                     /gene="rplQ"
                     /locus_tag="RHV13_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDV1241637.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15545..15913
                     /locus_tag="RHV13_002107"
     CDS             15545..15913
                     /locus_tag="RHV13_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HDV1241638.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGM
                     STDFLRGDYADKLLNKINDN"
     gene            15924..16349
                     /gene="zntR"
                     /locus_tag="RHV13_002108"
     CDS             15924..16349
                     /gene="zntR"
                     /locus_tag="RHV13_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HDV1241639.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16405..16623
                     /gene="arfA"
                     /locus_tag="RHV13_002109"
     CDS             16405..16623
                     /gene="arfA"
                     /locus_tag="RHV13_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HDV1241640.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGVC"
     gene            complement(16620..17030)
                     /gene="mscL"
                     /locus_tag="RHV13_002110"
     CDS             complement(16620..17030)
                     /gene="mscL"
                     /locus_tag="RHV13_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDV1241641.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17160..18536)
                     /gene="trkA"
                     /locus_tag="RHV13_002111"
     CDS             complement(17160..18536)
                     /gene="trkA"
                     /locus_tag="RHV13_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HDV1241642.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18558..19847)
                     /gene="rsmB"
                     /locus_tag="RHV13_002112"
     CDS             complement(18558..19847)
                     /gene="rsmB"
                     /locus_tag="RHV13_002112"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDV1241643.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNTSDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRINRTHHSRDSWLALLGEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19893..20840)
                     /gene="fmt"
                     /locus_tag="RHV13_002113"
     CDS             complement(19893..20840)
                     /gene="fmt"
                     /locus_tag="RHV13_002113"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDV1241644.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            complement(20855..21364)
                     /gene="def"
                     /locus_tag="RHV13_002114"
     CDS             complement(20855..21364)
                     /gene="def"
                     /locus_tag="RHV13_002114"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDV1241645.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21494..22618
                     /gene="dprA"
                     /locus_tag="RHV13_002115"
     CDS             21494..22618
                     /gene="dprA"
                     /locus_tag="RHV13_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDV1241646.1"
                     /translation="MVDTEIWLRLMSVSSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLAKHGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLATSLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22590..23063
                     /locus_tag="RHV13_002116"
     CDS             22590..23063
                     /locus_tag="RHV13_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HDV1241647.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23092..23634
                     /locus_tag="RHV13_002117"
     CDS             23092..23634
                     /locus_tag="RHV13_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HDV1241648.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23639..24211
                     /gene="tsaC"
                     /locus_tag="RHV13_002118"
     CDS             23639..24211
                     /gene="tsaC"
                     /locus_tag="RHV13_002118"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HDV1241649.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24216..25034
                     /gene="aroE"
                     /locus_tag="RHV13_002119"
     CDS             24216..25034
                     /gene="aroE"
                     /locus_tag="RHV13_002119"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDV1241650.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFV
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQTLSMGELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KIPFLAWCEQRGSKRTADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25031..25288
                     /locus_tag="RHV13_002120"
     CDS             25031..25288
                     /locus_tag="RHV13_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HDV1241651.1"
                     /translation="MNQAIQFPDREEWVENKKCVCFPALVNGMQLTCAISGDSLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25264..25818)
                     /locus_tag="RHV13_002121"
     CDS             complement(25264..25818)
                     /locus_tag="RHV13_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDV1241652.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLIIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6714 a         6181 c         6769 g         6455 t
ORIGIN      
        1 aaagttctgg gctaattaca cgttaattag ttttgaattg aaaagggcgc ttcggcgccc
       61 tttttgctgc ccgtaatttg agctcgaaat atttagtcgt aattttgctc gctgaactat
      121 ggcgcaaata aaactgtgct attgtaatca taactattct catttacact ttgcgcagaa
      181 tttgaacggg agcaatcatg tacgtttgtt tgtgtaatgg tgtaagtgac aaaaaaattc
      241 gccaggcagt ccgtcagttt catccgcaat ctttccaaca attgcgtaag tttattcctg
      301 tgggaaatca atgtggtaag tgcattcgcg ccgcgcgcga agtgatgcag gacgagttga
      361 tgcagatgcc agaatttaaa gagattgctt gaagcccacg cttatttttt gacaaccagc
      421 tagccccatc tacgctctaa tagtggaagc ggagggacta taaaatgaaa ggtgatgtta
      481 aaatcataaa ttatctcaat aaactattgg gaaatgagct tgtcgcaatc aatcagtatt
      541 ttctccatgc ccggatgttt aaaaactggg ggctgatgcg tctcaatgac gttgaatacc
      601 atgaatccat cgatgagatg aaacacgctg ataagtatat cgagcgtatt ttgtttttag
      661 aaggagtccc caacttacag gatttaggca aactggggat tggtgaagat gtcgaagaga
      721 tgctgcaatc cgacctgcga ttagagctgg aaggcgcgaa ggatctgcgt gaggctatcg
      781 catatgccga cagcgttcat gactatgtta gccgcgatat gatgatcgag atccttgctg
      841 atgaagaagg tcatatcgac tggctcgaaa ctgaactgga tctcatcggt aaaatcggcc
      901 tacaaaatta cctgcaatca caaattaaag ttaaagatta acggcctatc agcaaactat
      961 cccagcctat aacgaaaccc gcagccgcca gaaatggccc aaaaggcagc gggtttttta
     1021 atgattcttt tcctctcatg agcagaccaa taactacggc tccgcaagcg aatgaggcag
     1081 caaggaaaac cagtcgtggt aagaaggccc atgaatgcca ggcgccaagg gcggcgagaa
     1141 acttcacatc accatagcct aatccttctt tatggcgcag tatgcgatac ccccagtaaa
     1201 tgacagcgaa tgtgccgtaa ccaataattg ccccccatag cgcatcggct aaaccatcag
     1261 gatgacaaac ttgatagaac aaaagacctg accagagtaa cgggcacgta aatctgtcag
     1321 gtaacaagcc atgctttgca tcccagaaaa aaagtagggc tgataaacaa gcgtacaaaa
     1381 ttagaaaggg aagggtagtg tccataaaac atcctctgta attggagtat caccatcgaa
     1441 taactccgga tgctcagcaa cagagagagc gctgatttgt taacctgatt ccggtgaaaa
     1501 acgctatctt cacaacctta caaatttcgt ggaatgcagc tcgcaatctg cgcattgcga
     1561 ttaatttcta cccgatgctt gcgtcaggct cagatttgcg tataatgcgc gggcttgtcg
     1621 tagttgacag caggttcaat ctgaacctca gttagccgaa tttggctacc taacaatgct
     1681 cccaatcggg gagctacgta agaacggtta cactctccca tcaatcgtaa tgggtctgag
     1741 gagtaatcat tttcgtttat aaaataattg gagctctggt ctcatgcaga accaaagaat
     1801 ccgtatccgc ctgaaagcgt ttgatcatcg tctgatcgat caagcaaccg cggaaatcgt
     1861 cgagactgcc aagcgcactg gtgcgcaggt ccgtggtccg atcccgctgc cgacacgcaa
     1921 agagcgcttc actgttctga tctccccgca cgtcaacaaa gacgcgcgcg atcagtacga
     1981 aatccgtact cacttgcgtc tggttgacat cgttgagcca accgagaaaa ccgttgatgc
     2041 tctgatgcgt ctggatctgg ctgccggtgt agacgtgcag atcagcctgg gttaatcagg
     2101 tcattgagcg attgagaggt tgaaacaatg attggtttag tcggtaaaaa agtgggtatg
     2161 acccgtatct tcacagaaga cggcgtttct atcccagtaa ccgtaatcga agttgaagca
     2221 aaccgcgtta ctcaggttaa agacctggct aacgatggct accgtgctat tcaggtgacc
     2281 accggtgcta aaaaagctaa ccgtgtgacc aagcctgaag ctggccactt cgctaaagct
     2341 ggcgtagaag ctggccgtgg tctgtgggaa ttccgcctgg ctgaaggcga agagttcact
     2401 gtaggtcaga gcattagcgt tgaactgttt gctgacgtta aaaaagttga cgtaactggc
     2461 acctctaaag gtaaaggttt cgcaggtacc gttaagcgct ggaacttccg tacccaggac
     2521 gctactcacg gtaactcctt gtctcaccgc gttccgggtt ctatcggtca gaaccagact
     2581 ccgggcaaag tgttcaaagg caagaaaatg gcaggtcaga tgggtaacga acgtgtaacc
     2641 gttcagagcc ttgacgtagt acgcgttgac gctgagcgca acctgctgct ggttaaaggt
     2701 gctgtcccgg gtgcaaccgg tagcgacctg atcgttaaac cagctgtgaa ggcgtaagga
     2761 gatagcaatg gaattagtat tgaaagacgc gcagagcgcg ctgactgttt ccgaaactac
     2821 cttcggtcgt gatttcaacg aagcgctggt tcaccaggtt gttgttgctt atgcagctgg
     2881 tgctcgtcag ggtactcgtg ctcagaagac tcgtgctgaa gtaactggtt ccggtaaaaa
     2941 accgtggcgc cagaaaggca ccggccgtgc gcgttctggt tctatcaaga gcccgatctg
     3001 gcgttctggt ggcgtgacct ttgctgctcg tccgcaggac cacagtcaaa aagttaacaa
     3061 gaagatgtac cgcggcgcgc tgaaaagcat cctgtccgaa ctggtacgtc aggatcgtct
     3121 gatcgttgtc gagaagttct ctgtagaagc gccgaaaact aagctgctgg cacagaaact
     3181 gaaagacatg gctctggaag atgtgctgat catcaccggt gagctggacg aaaacctgtt
     3241 cctggctgcg cgcaacctgc acaaggttga cgtacgcgat gcaactggta tcgacccggt
     3301 tagcctgatc gccttcgaca aagtcgtaat gactgctgat gctgttaagc aagttgagga
     3361 gatgctggca tgattcgtga agaacgtctg ctgaaggtgc tgcgtgcacc gcacgtttct
     3421 gaaaaagcgt ctactgcgat ggaaaaatcc aacaccatcg tactcaaagt tgctaaagac
     3481 gcgaccaaag cagaaatcaa agctgctgtg cagaaactgt ttgaagtcga agtcgaagtc
     3541 gttaacaccc tggtagttaa agggaaagtt aaacgtcacg gacagcgtat cggtcgtcgt
     3601 agcgactgga aaaaagctta cgtcaccctg aaagaaggcc agaatctgga cttcgttggc
     3661 ggcgctgagt aagtcggagg agtaatacaa tggcagttgt taaatgtaaa ccgacatctc
     3721 cgggtcgtcg ccacgtagtt aaagtggtta accctgagct gcacaagggc aaaccttttg
     3781 ctccgttgct ggaaaaaaac agcaaatccg gtggtcgtaa caacaatggc cgtatcacca
     3841 ctcgtcatat cggtggtggc cacaagcagg cttaccgtat tgttgacttc aaacgcaaca
     3901 aagacggtat cccggcagtt gttgaacgtc ttgagtacga tccgaaccgt tccgcgaaca
     3961 tcgcgctggt tctgtacaaa gacggtgaac gccgttacat cctggcccct aaaggcctga
     4021 aagctggcga ccagattcag tctggcgttg atgctgcaat caaaccaggt aacaccctgc
     4081 cgatgcgcaa catcccggtt ggttctactg ttcataacgt agaaatgaaa ccaggtaaag
     4141 gcggtcagct ggcacgttcc gctggtactt acgttcagat cgttgctcgt gatggtgctt
     4201 atgtcaccct gcgtctgcgt tctggtgaaa tgcgtaaagt agaagcagac tgccgtgcaa
     4261 ctctgggcga agttggcaat gctgagcata tgctgcgcgt tctgggtaaa gcaggtgctg
     4321 cacgctggcg tggtgttcgt ccgaccgttc gcggtaccgc gatgaacccg gtagaccacc
     4381 cacatggtgg tggtgaaggt cgtaactttg gtaagcaccc ggtaactccg tggggcgttc
     4441 agaccaaagg taagaagacc cgcagcaaca agcgtactga taaattcatc gtacgtcgcc
     4501 gtagcaaata attttagagg ataagccatg ccacgttctc tcaagaaagg tccttttatt
     4561 gacctgcact tgctgaagaa ggtagagaaa gcggtggaaa gcggagacaa gaagcccctg
     4621 cgcacttggt cccgtcgttc aacgatcttt cctaacatga tcggtttgac catcgctgtc
     4681 cataatggtc gtcagcacgt tccggtattt gtaaccgacg aaatggtcgg tcacaaactg
     4741 ggtgaattcg caccgactcg tacttatcgc ggccacgctg ctgataaaaa agcgaagaag
     4801 aaataaggta ggaggaagag atggaaacta tcgctaaaca tcgccatgct cgttcttctg
     4861 ctcagaaggt tcgccttgtt gctgacctga ttcgcggtaa gaaagtgtcg caggctctgg
     4921 atattttgac ctacaccaac aagaaagcgg ctgtactggt caagaaagtt ctggaatctg
     4981 ccattgctaa cgctgaacac aacgatggcg ctgacattga cgatctgaaa gttacgaaaa
     5041 ttttcgtaga cgaaggcccg agcatgaagc gcattatgcc gcgtgcaaaa ggtcgtgcag
     5101 atcgcatcct gaagcgcacc agccacatca ctgtggttgt gtccgatcgc tgagactctg
     5161 gagactagca atgggtcaga aagtacatcc taatggtatt cgcctgggta ttgtaaaacc
     5221 atggaactct acctggtttg cgaacaccaa agaattcgct gacaacctgg acagcgattt
     5281 taaagtacgt cagtacctga ctaaggaact ggctaaagcg tccgtatctc gtatcgttat
     5341 cgagcgtccg gctaagagca tccgtgtaac cattcacact gctcgcccgg gtatcgttat
     5401 cggtaaaaaa ggtgaagacg tagaaaaact gcgtaaggtc gtagcggaca tcgctggcgt
     5461 tcctgcacag atcaacatcg ccgaagttcg taagcctgaa ctggacgcaa aactggttgc
     5521 tgacagcatc acttctcagc tggaacgtcg cgttatgttc cgtcgtgcta tgaagcgtgc
     5581 tgtacagaac gcaatgcgtc tgggcgctaa aggtattaaa gttgaagtta gcggccgtct
     5641 gggcggcgcg gaaatcgcac gtaccgaatg gtaccgcgaa ggtcgcgtac cgctgcacac
     5701 tctgcgtgct gacatcgact acaacacctc tgaagcgcac accacttacg gtgtaatcgg
     5761 cgttaaagtg tggatcttca aaggcgagat cctgggtggt atggctgctg ttgaacaacc
     5821 ggaaaaaccg gctgctcagc ctaaaaagca gcagcgtaaa ggccgtaaat aaggagcgtc
     5881 gctgatgtta caaccaaagc gtacaaaatt ccgtaaaatg cacaaaggcc gtaaccgcgg
     5941 tctggcgcag ggtacggatg ttagcttcgg cagcttcggt ctgaaagctg ttggccgtgg
     6001 tcgtctgact gcccgtcaga tcgaagcagc acgtcgtgct atgacccgtg cagttaagcg
     6061 tcaaggtaag atctggatcc gtgtgttccc ggacaaaccg atcactgaaa agccgctggc
     6121 agtgcgtatg ggtaaaggta aaggtaacgt ggagtattgg gttgccttga ttcagccggg
     6181 taaagtcctg tatgaaatgg acggtgttcc ggaagagctg gcccgtgaag cattcaagct
     6241 ggcagcagcg aaactgccga ttaaaaccac ctttgtaact aagacggtga tgtaatgaaa
     6301 gcaaaagagc tgcgtgagaa gagcgttgaa gagctgaaca ccgagctgct gaacctgctg
     6361 cgtgagcagt tcaacctgcg tatgcaggct gcaagtggcc agctgcaaca gtctcacctg
     6421 ttgaagcaag tgcgtcgcga tgtcgcacgc gttaagactt tactgaacga gaaggcgggt
     6481 gcgtaatgac cgataaaatc cgtactctgc aaggtcgcgt tgttagcgac aaaatggaga
     6541 aatccattgt tgttgctatc gaacgttttg tgaaacaccc gatctacggt aaattcatca
     6601 agcgtacgac caaactgcac gtacatgacg agaacaacga atgcggtatc ggtgacgtgg
     6661 ttgaaatccg cgaatgccgt ccgctgtcca agactaaatc ctggacgctg gttcgcgttg
     6721 tagagaaagc ggttctgtaa tacagtacac tctctcgata cgaataaacg gctcagaaat
     6781 gagccgttta ttttttctac ccatatcctt gaagcggtgt tataatgccg cgccctcgat
     6841 atggggattt ttaacgacct gattttcggg tctcagtagt agttgacatt agcggagcac
     6901 taaaatgatc caagaacaga ctatgctgaa cgtcgccgac aactccggtg cacgtcgcgt
     6961 aatgtgtatc aaggttctgg gtggctcgca ccgtcgctac gcaggcgtag gcgacatcat
     7021 caagatcacc atcaaagaag caattccgcg tggtaaggtc aaaaaaggtg atgtgctgaa
     7081 ggcggtagtg gtgcgcacca agaagggtgt tcgtcgcccg gacggttctg tcattcgctt
     7141 cgatggtaat gcttgtgttc ttctgaacaa caacagcgag cagcctatcg gtacgcgtat
     7201 ttttgggccg gtaactcgtg agcttcgtag tgagaagttc atgaaaatta tctctctggc
     7261 accagaagta ctctaaggag cgaatcatgg cagcgaaaat ccgtcgtgat gacgaagtta
     7321 tcgtgttaac cggtaaagat aaaggtaaac gcggtaaagt taagaatgtc ctgtcttccg
     7381 gcaaggtcat tgttgaaggt atcaacctgg ttaagaaaca tcagaagccg gttccggccc
     7441 tgaaccaacc gggtggcatc gttgaaaaag aagccgctat tcaggtttcc aacgtagcaa
     7501 tcttcaatgc ggcaaccggc aaggctgacc gtgtaggctt tagattcgaa gacggcaaaa
     7561 aagtccgttt cttcaagtct aatagcgaaa ctatcaagta atttggagta gtacgatggc
     7621 gaaactgcat gattactaca aagacgaagt agttaaaaaa ctcatgactg agtttaacta
     7681 caattctgtc atgcaagtcc ctcgggtcga gaagatcacc ctgaacatgg gtgttggtga
     7741 agcgatcgct gacaaaaaac tgctggataa cgcagcagca gacctggcag caatctccgg
     7801 tcaaaaaccg ctgatcacca aagcacgcaa atctgttgca ggcttcaaaa tccgtcaggg
     7861 ctatccgatc ggctgtaaag taactctgcg tggcgaacgc atgtgggagt tctttgagcg
     7921 cctgatcact attgctgtac ctcgtatccg tgacttccgt ggcctgtccg ctaagtcttt
     7981 cgacggtcgt ggtaactaca gcatgggtgt ccgtgagcag atcatcttcc cagaaatcga
     8041 ctacgataaa gtcgaccgcg ttcgtggttt ggatattacc attaccacta ctgcgaaatc
     8101 tgacgaagaa ggccgcgctc tgctggctgc ctttgacttc ccgttccgca agtaaggtag
     8161 ggttactaaa tggctaagca atcaatgaaa gcacgcgaag taaaacgcgt agctttagct
     8221 gataaatact tcgcgaaacg cgctgaactg aaagcgatca tctctgatgt gaacgcttcc
     8281 gacgaagatc gttggaacgc tgttctcaag ctgcagactc tgccgcgtga ttccagcccg
     8341 tctcgtcagc gtaaccgctg ccgtcaaaca ggtcgtccgc atggtttcct gcggaagttc
     8401 gggttgagcc gtattaaggt ccgtgaagcc gctatgcgcg gtgaaatccc gggtctgaaa
     8461 aaggctagct ggtaattgtc accaattgaa tcacgggagt aaagacagat gagcatgcaa
     8521 gatccgatcg cggatatgct gacccgtatc cgtaacggtc aggccgcgaa caaagctgcg
     8581 gtcaccatgc cttcctccaa gctgaaagtg gcaatcgcca acgtgctgaa ggaagaaggt
     8641 tttattgaag attttaaagt tgaaggcgac accaagcctg aactggaact tactctgaag
     8701 tatttccagg gcaaagctgt tgtagaaagc attcagcgtg tcagccgccc aggtctgcgc
     8761 atctataaac gtaaagatga gctgccgaaa gttatggcgg gtctgggtat cgcagttgtt
     8821 tctacctcta aaggtgttat gactgatcgt gcagcgcgcc aggctggtct tggtggcgaa
     8881 attatctgct acgtagccta atcggaggaa aaaatgtctc gtgttgctaa agcaccggtc
     8941 gttgttcctg ccggcgttga cgtaaaaatc aacggtcagg ttattacgat caaaggtaaa
     9001 aacggcgagc tgactcgtac tctcaacgat gctgttgaag ttaaacatgc agataatacc
     9061 ctgaccttcg gtccgcgtga tggttacgca gacggttggg cacaggctgg taccgcgcgt
     9121 gccctgctga actcaatggt tatcggtgtt accgaaggct tcactaagaa gctgcagctg
     9181 gttggtgtag gttaccgtgc agcggttaaa ggcaatgtga ttaacctgtc tctgggtttc
     9241 tctcatcctg ttgaccatca gctgcctgcg ggtatcactg ctgaatgtcc gactcagact
     9301 gaaatcgtgc tgaaaggcgc tgataagcag gtgatcggcc aggttgcagc ggatctgcgc
     9361 gcctaccgtc gtcctgagcc ttataaaggc aagggtgttc gttacgccga cgaagtcgtg
     9421 cgtaccaaag aggctaagaa gaagtaaggt aacactatgg ataagaaatc tgctcgtatc
     9481 cgtcgtgcga cccgcgcacg ccgcaagctc caggagctgg gcgcaactcg cctggtggta
     9541 catcgtaccc cgcgtcacat ttacgcacag gtaattgcac cgaacggttc tgaagttctg
     9601 gtagctgctt ctactgtaga aaaagctatc gctgaacaac tgaagtacac cggtaacaaa
     9661 gacgcggctg cagctgtggg taaagctgtc gctgaacgcg ctctggaaaa aggcatcaaa
     9721 gatgtatcct ttgaccgttc cgggttccaa tatcatggtc gtgtccaggc actggcagat
     9781 gctgcccgtg aagctggcct tcagttctaa ggtagaggtg taagatggct cacatcgaaa
     9841 aacaagctgg cgaactgcag gaaaagctga tcgcggtaaa ccgcgtatct aaaaccgtta
     9901 aaggtggtcg tattttctcc ttcacagctc tgactgtagt tggcgatggt aacggtcgcg
     9961 ttggttttgg ttacggtaaa gcgcgtgaag ttccagcagc gatccagaaa gcgatggaaa
    10021 aagcccgtcg caatatgatt aacgtcgcgc tgaataacgg cactctgcaa caccctgtta
    10081 aaggtgttca cacgggttct cgcgtattca tgcagccggc ttccgaaggt accggtatca
    10141 tcgccggtgg tgcaatgcgc gccgttctgg aagtcgctgg ggttcataac gttctggcta
    10201 aagcatatgg ttccaccaac ccgatcaacg tggttcgtgc aactattgat ggcctggaaa
    10261 atatgaattc tccagaaatg gtcgctgcca agcgtggtaa atccgttgaa gaaattctgg
    10321 ggaaataaac catggcaaag actattaaaa ttactcaaac ccgcagtgca atcggtcgtc
    10381 tgccgaaaca caaggcaacg ctgcttggcc tgggtctgcg tcgtattggt cacaccgtag
    10441 agcgcgagga tactcctgct attcgcggta tgatcaacgc ggtttccttc atggttaaag
    10501 ttgaggagta agagatgcgt ttaaatactc tgtctccggc cgaaggctcc aaaaaggcgg
    10561 gtaaacgcct gggtcgtggt atcggttctg gcctcggtaa aaccggtggt cgtggtcaca
    10621 aaggtcagaa gtctcgttct ggcggtggcg tacgtcgcgg tttcgagggt ggtcagatgc
    10681 ctctgtaccg tcgtctgccg aaattcggct tcacttctcg taaagcagcg attacagccg
    10741 aagttcgtct gtctgacctg gctaaagtag aaggcggtgt tgtagacctg aacacgctga
    10801 aagcggctaa cattatcggt atccagatcg agttcgcgaa agtgatcctg gctggcgaag
    10861 taacgactcc ggtaactgtt cgtggcctgc gtgttactaa aggcgctcgt gctgctatcg
    10921 aagctgctgg cggtaaaatc gaggaataag tagcagatgg ctaaacaacc gggattagat
    10981 tttcaaagtg ccaaaggtgg cttaggcgag ctgaaacgca gactgctgtt tgttatcggt
    11041 gcgctgattg tgttccgtat tggctctttt attccgatcc ctggtattga tgccgctgta
    11101 cttgccaaac tgcttgagca acagcgaggc accatcattg agatgtttaa catgttctct
    11161 ggtggtgctc tcagccgtgc ttctatcttt gctctgggga tcatgccgta tatttcggcg
    11221 tcgatcatta tccagctgct gacggtggtt cacccaacgt tggcagaaat taagaaagaa
    11281 ggggagtctg gtcgtcgtaa gatcagccag tacacccgct acggtactct ggtgctggca
    11341 atattccagt cgatcggtat tgctaccggt ctgccgaata tgcctggtat gcaaggcctg
    11401 gtgattaacc cgggctttgc attctacttc accgctgttg taagtctggt cacaggaacg
    11461 atgttcctga tgtggttggg cgaacagatc actgaacgag gtatcggcaa cggtatttca
    11521 atcattatct tcgccggtat tgtcgcggga ctcccgccag ccattgccca tactatcgag
    11581 caagcgcgtc aaggcgacct gcacttcctc gtgttgctgt tggttgcagt attagtattt
    11641 gcagtgacgt tctttgttgt atttgttgag cgtggtcaac gccgcattgt ggtaaactac
    11701 gcgaaacgtc agcaaggtcg tcgtgtctat gctgcacaga gcacacattt accgctgaaa
    11761 gtgaatatgg cgggggtaat cccggcaatc ttcgcttcca gtattattct gttcccggcg
    11821 accatcgcgt catggttcgg gggcggtact ggttggaact ggctgacaac aatttcgctg
    11881 tatttgcagc ctgggcaacc gctttatgtg ttactctatg cgtctgcaat catcttcttc
    11941 tgtttcttct acacggcgtt ggttttcaac ccgcgtgaaa cagcagataa cctgaagaag
    12001 tccggtgcat ttgtaccagg aattcgtccg ggagagcaaa cggcgaagta tatcgataaa
    12061 gtaatgaccc gcctgaccct ggttggtgcg ctgtatatta cctttatctg cctgatcccg
    12121 gagttcatgc gtgatgcaat gaaagtaccg ttctacttcg gtgggacctc actgcttatc
    12181 gttgttgtcg tgattatgga ctttatggct caagtgcaaa ctctgatgat gtccagtcag
    12241 tatgagtctg cattgaagaa ggcgaacctg aaaggctacg gccgataatt ggtcgcccga
    12301 gaagttacgg agagtaaaaa tgaaagttcg tgcttccgtc aagaaattat gccgtaactg
    12361 caaaatcgtt aagcgtgatg gtgtcatccg tgtgatttgc agtgccgagc cgaagcataa
    12421 acagcgccaa ggctgatttt ttcgcatatt tttcttgcaa agttgggttg agctggctag
    12481 attagccagc caatcttttg tatgtctgtg cgtttccatt tgagtatcct gaaaacgggc
    12541 ttttcagcat ggaacgtaca tattaaatag taggagtgca tagtggcccg tatagcaggc
    12601 attaacattc ctgatcataa gcatgccgta atcgcattaa cttcgattta tggcgtcggc
    12661 aagacccgtt ctaaagccat cctggctgca gcgggtatcg ctgaagatgt taagatcagt
    12721 gagctgtctg aaggacaaat cgacacgctg cgtgacgaag ttgccaaatt tgtcgttgaa
    12781 ggtgatctgc gccgtgaaat cagcatgagc atcaagcgcc tgatggatct tggttgctat
    12841 cgcggtttgc gtcatcgtcg tggtctcccg gttcgcggtc agcgtaccaa gaccaacgca
    12901 cgtacccgta agggtccgcg caaaccgatc aagaaataat cggggtgatt gaataatggc
    12961 aaaggcacca attcgtgcac gtaaacgtgt aagaaaacaa gtctctgacg gcgtggctca
    13021 tatccatgct tctttcaaca acaccatcgt gactatcact gatcgtcagg gtaacgcgtt
    13081 gggttgggca acagccggtg gttccggttt ccgtggttct cgcaaatcca ctccgtttgc
    13141 agctcaggtt gcagcagagc gttgcgctga cgccgtgaaa gaatacggca tcaagaatct
    13201 ggaagttatg gttaaaggtc cgggtccagg ccgcgaatct actattcgtg ctctgaacgc
    13261 cgcaggtttc cgcatcacta acattactga tgtgactccg atccctcata acggttgtcg
    13321 tccgccgaaa aaacgtcgcg tataacgcct cgttttccag gtttgttgga gaaagaaaat
    13381 ggcaagatat ttgggtccta agctcaagct gagccgtcgt gagggcaccg acttattcct
    13441 taagtctggc gttcgcgcga tcgataccaa gtgtaaaatt gaacaagctc ctggccagca
    13501 cggtgcgcgt aaaccgcgtc tgtctgacta tggtgtgcag ttgcgtgaaa agcaaaaagt
    13561 tcgccgtatc tatggtgtgc tggagcgtca gttccgtaac tactacaaag aagcagcacg
    13621 tctgaaaggc aacaccggtg aaaacctgtt ggctctgctg gaaggtcgtc tggacaacgt
    13681 tgtataccgt atgggcttcg gtgccactcg tgcagaagca cgtcagctgg ttagccataa
    13741 agcaattatg gtaaacggtc gtgttgttaa catcgcttct tatcaggtta gtccgaatga
    13801 cgttgtaagc attcgtgaga aagcgaagaa gcagtctcgc gtgaaagccg ctctggagct
    13861 ggctgagcag cgtgaaaagc caacctggct ggaagttgat gctggcaaga tggaaggtac
    13921 gtttaagcgt aagccggagc gttctgatct gtctgcggac attaacgaac acctgatcgt
    13981 cgagctttac tccaagtaaa gcttagtacc aaagagagga cacaatgcag ggttctgtga
    14041 cagagtttct aaaaccgcgc ctggttgata tcgagcaagt gagttcgacg cacgccaagg
    14101 tgacccttga gcctttagag cgtggctttg gccatactct gggtaacgca ctgcgccgta
    14161 ttctgctctc atcgatgccg ggttgcgcgg tgaccgaggt tgagattgat ggtgtactac
    14221 atgagtacag caccaaagaa ggcgttcagg aagatatcct ggaaatcctg ctcaacctga
    14281 aagggctggc ggtgagagtt cagggcaaag atgaagttat tcttaccttg aataaatctg
    14341 gcattggccc tgtgactgca gccgatatca cccacgacgg tgatgtcgaa atcgtcaagc
    14401 cgcagcacgt gatctgccac ctgaccgatg agaacgcgtc tattagcatg cgtatcaaag
    14461 ttcagcgcgg tcgtggttat gtgccggctt ctacccgaat tcattcggaa gaagatgagc
    14521 gcccaatcgg ccgtctgctg gtcgacgcat gctacagccc tgtggagcgt attgcctaca
    14581 atgttgaagc agcgcgtgta gaacagcgta ccgacctgga caagctggtc atcgaaatgg
    14641 aaaccaacgg cacaatcgat cctgaagagg cgattcgtcg tgcggcaacc attctggctg
    14701 aacaactgga agctttcgtt gacttacgtg atgtacgtca gcctgaagtg aaagaagaga
    14761 aaccagagtt cgatccgatc ctgctgcgcc ctgttgacga tctggaattg actgtccgct
    14821 ctgctaactg ccttaaagca gaagctatcc actatatcgg tgatctggta cagcgtaccg
    14881 aggttgagct ccttaaaacg cctaaccttg gtaaaaaatc tcttactgag attaaagacg
    14941 tgctggcttc ccgtggactg tctctgggca tgcgcctgga aaactggcca ccggcaagca
    15001 tcgctgacga gtaaccggat cacaggttaa ggttttactg agaaggataa ggtcatgcgc
    15061 catcgtaaga gtggtcgtca actgaaccgc aacagcagcc atcgccaggc tatgttccgc
    15121 aatatggcag gttcactggt tcgtcatgaa atcatcaaga cgactctgcc taaagcgaaa
    15181 gagctgcgcc gcgtagttga gccgctgatt actcttgcca agactgatag cgttgctaat
    15241 cgtcgtctgg cattcgcccg tactcgtgat aacgagatcg tggcaaaact gtttaacgaa
    15301 ctgggcccgc gtttcgcgag ccgtgccggt ggttacactc gtattctgaa gtgtggcttc
    15361 cgtgcaggcg acaacgcgcc gatggcttac atcgagctgg ttgatcgttc agagaaagca
    15421 gaagctgctg cagagtaatc tgaagcaacg taaaaaaacc cgccccggcg ggttttttta
    15481 tacccgtagc atccccactt atctacaata gctgtactct ttttgttcat cccctggagt
    15541 atttatgtgg ttacttgacc agtgggcaga gcgtcatata gcagaagcgc aagcgaaagg
    15601 ggagtttgat aacctggcag gtagcggcga accattgata ctggatgatg attctcatgt
    15661 gccaccggaa ttacgtgcgg ggtatcgctt gctgaagaat gccggttgct taccgccaga
    15721 acttgagcaa cggagggaag caattcagct tctggatatt ctcaaaggta tccgtcacga
    15781 tgatccgcaa tatcaagagg ttagccgtcg attgtcatta ctggaattga agctgcgaca
    15841 agctggaatg agtaccgatt ttttacgcgg cgattatgct gacaagttgt tgaataaaat
    15901 caacgataac tagtggagta tgtatgtatc gcattggtga gctggcaaaa atggcggaag
    15961 taacacccga cacgattcgt tattacgaaa aacagcagat gatggagcat gaagtgcgta
    16021 ctgaaggtgg gtttcgcctg tataccgaaa gcgatctcca gcgattgaaa tttatccgcc
    16081 atgccagaca actaggtttc agtctggagt cgatccgcga gttgctgtcg atccgtatcg
    16141 atcctgaaca ccatacctgt caggagtcaa aaggcattgt gcaggaaaga ttgcaggaag
    16201 tcgaagcacg gatagccgag ttgcagagta tgcagcgttc cttgcaacgc cttaacgatg
    16261 cctgttgtgg gaccgcccat agcagtgttt attgctcgat tcttgaagcc cttgaacaag
    16321 gggcgagtgg cgttaagagt ggctgttgat tttttgcact gacaggatta cactcgcgcc
    16381 gttaaataac caactggagt ttttatgagc cgatatcagc atactaaagg gcagataaag
    16441 gataatgcga tagaagcatt actacatgat cccttattcc gacagcgagt agagaaaaat
    16501 aagaaaggga aaggcagtta catgcgaaaa ggaaaacatg gcaatcgggg gaactgggag
    16561 gccagtggca agaaagtaaa tcactttttt accactggcc ttctgctttc aggcgtttgt
    16621 taagagcggt tattctgctc tttcagcaaa tcacgaattt ctgtcagtaa tacttcttct
    16681 ttagttggtg ctggtgcggc tgctggttct tcttttttcc gattcagttt gttgattagc
    16741 ttaatcgcca taaagatggc aaaggccaca atcagaaaat caaagacgtt ttgaatgaag
    16801 acaccgtaat gcatcacaac agcagggata tccccctgcg catcgcgtag cgtgacagca
    16861 aactgtttaa agtcgatccc gccaattaat aagcccagag gtggcatgat gatatcggca
    16921 accagtgaag atacaatctt cccgaatgcc gcaccgataa tgacacccac tgccaaatcc
    16981 accacgttcc cgcgcatcgc aaattcgcga aattctttaa taatgctcat gttattctcc
    17041 ctatgaagcc gacaaccata agtctaacaa atgttaagcc attttcctgc caggaatgaa
    17101 tttaattagt ccttgataat caaagggaaa taataaggcg tcattagacg ccttattaat
    17161 tacaagaaga aagggcttgg ctggaagagt ctttcgacgt cggtaataaa ttttttatct
    17221 gtgaggaaca taattacgtg atcgccttgc tcaatgcgca gattgtcatt ggcaatcatc
    17281 acgtcgtttc cacgtaccac cgctccaata atcgttcctg gcggtagctt gatttcatca
    17341 atgactctgc cgacaacgcg tgaggtgctt tcatcaccgt gagcaacggc ttcaatagct
    17401 tctgctacgc cgcggcgcaa tgaggaaaca ccaacaatat ctgcttttcg cacatggcta
    17461 agcaacgcag aaatagttgc ttgttgtggt gaaatcgcaa tatcgatcac gctcccctga
    17521 accagatcca cataagcgcg acgctggatc aataccatca cctttttcgc acccatacgt
    17581 ttggcaagca tggcggacat gatattggcc tcgtcatcgt tggtgacggc aataaacaga
    17641 tcaacttgat cgatatgttc ttcggccagc agttcttgat ccgacgcatc accaaaaaag
    17701 acgatcgtat tctgcaactt ttccgccagt tcggcagcgc gctgctgatt acgttcgatg
    17761 agtttaacgc tgtaatcttt ttccagacga cgcgccagcc ctgcaccgat attaccgcca
    17821 ccaaccagca tgatccgctt atacggtttt tccagtcgct gtaattcact catcaccgcg
    17881 cggatatgct gtgaagcggc aataaagaac acttcatcac cagcctcaac aatggtcgaa
    17941 ccttgcggac gaatggggcg atcgtggcgg aaaattgctg ccacacgagt atcgatatgt
    18001 ggcatatgtt cgcgcatggt cgaaagtgca ttaccaatca gcgggccgcc ataataggct
    18061 ttaaccacag cgaggctgac tttaccctca gcgaagttca ccacctgcaa tgcgccggga
    18121 tactcaatca gtcggtaaat attatcgata accaactgct ctggtgcgat cagatgatca
    18181 atcggtacag catctgaatg aaatagctta tcggcatcgc gtacgtagtc tggtgagcgg
    18241 atacgagcga tgcgattagg ggtgttgaaa agtgagtagg ctacctggca ggcaaccata
    18301 ttggtttcat ctgaactggt tacagcaacc agcatatcgg cgtcgtcggc acctgcctcc
    18361 cgcaatacgc gtggatgaga gccatgcccc tgcacgactc gcaggtcaaa tttatcctgc
    18421 aaggttcgca gacgctcacc gttggtatcg acaacagtga tatcgttgtt ctcgccaacc
    18481 aggttttccg ccagtgtgcc gccaacctgg ccggcaccca gaatgataat tttcatcagt
    18541 cgcgacccgt tatctcatca ctttttgatt agcttagcgt aaaagaagcc gtcgccctct
    18601 tcggcaccag gcagattttg tttacccggt tgctctggtg ttcctgtttc gcaaagttcg
    18661 gcatcagcgg tacgttgcaa aaaggcttta atctgcaggc tattctcttc cggtaacacc
    18721 gaacaggtgg catagaccag ggttccaccg gattttaaat gcggccaaat ggcgtcgaga
    18781 atttcagact gcaattgcgc gagttccggg atatcgcgat cgcgacgtaa ccatttaata
    18841 tccggatggc gacgaatcac accggttgct gaacaaggcg catctaataa aatgcgatca
    18901 aactgttgct cgccacacca ttgggaaggg taacggccat caccttgttt cacggttgcc
    18961 ttcataccaa ggcgttttaa attgtcgtaa acgcgagaga ggcgctgttc gtcaatatca
    19021 accgcaacaa cctgcgcttc tggtgccact tcaaggatat gcgttgtttt accgccgggg
    19081 gcggcacaaa gatccagaat gtgttcaccg ttttgtggcg caagccaggt catgcaacct
    19141 tgtgctgatg catcctgaac ggtaacccat ccgtcttcaa aaccaggtag cgcatgaaca
    19201 ggtgcaggtg tttccagccg tacagcatca gggtaatccg catgcgggaa acctttcatt
    19261 cctgcttcac ccagcaatgc aagccagctg tcgcgggaat gatgcgtacg attaatacgc
    19321 agccacattg gcggacgctg gttattggct tcgacgatgg attgccactg ctctggatac
    19381 gctttttgca gacgcttcag caaccaggaa ggatgcagat aacgtgcatc actggtatta
    19441 aactcggcta ataactcttc ttgctgacgc tggaactggc gtaatacacc gtttatcaac
    19501 cctttaagtt gcggacgctt aattgcgata gcgccttcaa ccgtttcagc cagcgcagca
    19561 tgaggtggaa tgcgggtata aagcagttga tacaaaccaa ccataatcag gtaatgcaca
    19621 gtgcgctgtt tgccggtcat cggacgggcc attaacttat taatcagcca gtctaactgc
    19681 gaaagcgtac gcagtacgcc aaagcacaac tcttgaagaa gtgctttgtc tttatcggaa
    19741 actttttgct ggagcggtgg cagaatgttg cttaatgatt gcccttgctc gacgacttgt
    19801 tcaacggcct gggccgccat gctacgtaaa ttacgttgtt ttttcataag tataaaaatg
    19861 cccggcaaga ccgggcttag aagagtggac tatcaggcca gacggttgcc cggaacaaac
    19921 cattcccgac gagaattcag gaggtcttgc gcgctcatcg ctttcttacc cgcaggttgt
    19981 aacgagagca ggttcaggat tccatcacca gtcgcaacct gaatgccttg tttgttggct
    20041 tcaaggatcg ttcctggtgc agcgttggtt gccgtatcaa tgaccgacgc tttccagact
    20101 ttaaccggct gtccttcaat ttccagccag ctcattggcc atggattgaa agcgcgaata
    20161 cagcgttcaa gctgcgctgc cgaaagtgac cagtcaatac gcgcttcttc tttactcaac
    20221 ttttcggcat aagtgacaag agtttcgtcc tgaacttctg gtttcgccgt gccgtctgcc
    20281 agttgtttca acgtggtgat aagcccttgt gggccaagct ctgccagctt gtcgtacagc
    20341 gtaccactgg tatcttctgc agtaatcggg caggagagct tatagagcat gtcaccggtg
    20401 tctaaaccaa catccatttg cataatggtc acaccagttt ctgcatcacc cgcccatagt
    20461 gagcgttgga ttggtgcagc accgcgccag cgtggcagca gtgaaccatg aacgttgata
    20521 cagccaagac gcggcatctc cagcactgct ttaggcagaa ttaagccata ggcgacgacg
    20581 accataacat cagcctgcag atcggcgacc agttgctggt tttcttgtgg acgcagggaa
    20641 acaggttgaa aaacgggcag acctttttcc tcagccagaa ctttaatcgg gctgggcatc
    20701 agttttttac cgcgtcctgc cggtcggtct ggctgggtga acacgccaac gacgttatga
    20761 ccagaagaca acagcgcgtc gagatgacgc gctgcaaagt caggtgtacc cgcaaaaata
    20821 atacgtagtg attctgacac gttagttctt atccttaagc ccgggctttc agacgatcca
    20881 gtttttcaac tttctgacga atacgttgtt gtttcagcgg tgacagataa tccataaaca
    20941 gtttgccgac caggtgatcc atctcatgct gaatacagat ggctaacaga ccatctgctt
    21001 ccagttcaaa tggtttaccg tcgcggtcca gggcgcgaat tttaactttc tctgcgcgtg
    21061 gcactaaagc acgttgttca gggatcgaca ggcatccttc ttcaatgcct gtttcgccgc
    21121 ttttttctaa aagctccgga ttgattaaca ccagccgttc gtcacggttt tccgaaacat
    21181 caataacaat gatacgttga tggatatcaa cctgggttgc cgccaggcca ataccttctt
    21241 ctgcgtacat cgtctcgaac atatcatcga cgatacgctg aatttctgca ttcacttctt
    21301 ctaccggttt agcaactttg cgaagccgct cgtccggaat atgtaacact tgcaaaactg
    21361 acataaatct ccagagatgt gttcaggagt tagaaagatt atttcttcta ttctagacaa
    21421 atccccctct gattgacagc atcactgacc aatcgcaaag attgctaagg ctgcttatgg
    21481 cagggagata aggatggtcg atacagaaat ttggctgcgt ttaatgagcg tcagcagctt
    21541 gtacggcgat gatatggtcc gtatcgctca ctggctggca aaacagtcgc atattgatgc
    21601 ggttgtattg cagcaaacag ggcttacatt gcggcaggca caacgctttc tttcatttcc
    21661 acgaaagagt atcgaaagct cactttgttg gttggagcaa cccaaccatc atttaatccc
    21721 tgcggacagc gaattttatc ctcctcaact tctggcgacg acagattacc ccggcgcact
    21781 gtttgttgaa ggagaactgc acgcgctgca ttcatttcag cttgccgtag tggggagtcg
    21841 ggcgcattca tggtatggcg agcgatgggg acgattattt tgcgaaactc tggcgaagca
    21901 tggagtgaca attacgagtg gactggcgcg tggaatcgat ggtgtggcgc ataaagctgc
    21961 cttacaggta aatggcgtca gcattgctgt attggggaac ggacttaata ccattcatcc
    22021 ccgccgccat gcccgactgg ctaccagttt gcttgaacat ggtggggcac ttgtctcgga
    22081 atttcccctc gatgttcctc cccttgctta caatttccca cgaagaaatc gcattatcag
    22141 tggtctaagt aaaggtgtac tggtggtgga agcggctttg cgcagtggtt cgctggtgac
    22201 agcacgttgt gcgcttgagc aggggcgtga agtttttgcc ttgccaggac caatagggaa
    22261 tccgggaagc gaagggcctc actggttaat aaaacaaggt gcgattcttg tgacggaacc
    22321 ggaagaaatt ctggaaaact tgcaatttgg attgcactgg ttgccagacg cccctgaaaa
    22381 ttcattttat tcaccagatc agcaagacgt ggcattgcca tttcctgagc tcttggctaa
    22441 cgtaggagat gaggtaacac ctgttgacgt cgtcgctgaa cgtgccggcc aacctgtgcc
    22501 agaggtagtt actcaactac tcgaactgga gttagcagga tggatcgcag ctgtacccgg
    22561 cggctatgtc cgattgagga gggcatgcca tgttcgacgt actaatgtat ttgtttgaaa
    22621 cctatattca cacagaagct gagttgcgtg tggatcaaga caaacttgaa caggatctta
    22681 ccgacgcagg atttgatcga gaagatatct acaatgccct gctttggctg gaaaagcttg
    22741 cggattatca ggaaggactg gcagaaccga tgcaactggc ctctgatcca ctctccatgc
    22801 gtatttatac ccctgaggag tgtgaaagac tggatgccag ctgccgtggt tttctgcttt
    22861 tccttgagca aattcaggtg cttaaccttg aaacccgtga aatggtgata gagcgagtgc
    22921 tcgcgctgga taccgcagag ttcgacctgg aagacctgaa atgggtaatc ctgatggtgt
    22981 tgttcaatat tccgggctgc gaaaatgcgt accagcaaat ggaagaatta ctctttgaag
    23041 tgaatgaagg tatgctgcat taatcatttc tttaattcag cataagttgt tatggcgaaa
    23101 tcagcactgt tcacggtgcg taataatgag tcctgcccaa agtgcggggc tgaactggtt
    23161 attcgatccg ggaaacacgg tccgtttctt gggtgctcac agtatccggc gtgtgactac
    23221 gtccgtcctc tgaaatcttc agcggatggg catatcgtca aagttctgga ggggcaggtt
    23281 tgccctgcat gtggcgcaaa tctggtatta cgccagggac gctttggtat gtttattggt
    23341 tgcagtaact acccagaatg cgaacatacc gaacttatcg ataaaccgga cgaaacagca
    23401 attacctgtc ctcaatgtcg gacgggccat ctggtccagc gccgctcccg ttatggcaaa
    23461 acatttcact cctgtgatcg ctaccctgag tgtcaatttg ctattaactt caaaccaata
    23521 gccggagagt gccctgagtg tcattatccg ctactcatcg aaaagaaaac cgcgcagggt
    23581 gtaaaacact tttgtgccag taaacaatgt ggaaagccgg tttcggcgga ataataacgt
    23641 gaataataac ctgcaaggag acgctatcgc agctgcgata gatgtcctca atgaagaacg
    23701 tgtcatcgcc tatccaacgg aagctgtttt cggtgtcggc tgcgatcctg atagcgaaac
    23761 agcagtgatg agactgttgg agttaaaaca gcgtccggtt gataaggggc tgattttaat
    23821 cgcggcaaat tacgagcagc ttaaacccta tattgatgac accatgctga ctgatgcgca
    23881 gcgtgaaacc attttttccc gctggccagg tcctgtcacc tttgtctttc ccgcgcctgc
    23941 gacaacaccg cgctggttga cgggccgctt tgattcgctt gctgtacgag tcaccgacca
    24001 tccgttggtg gttgctttgt gccaggctta tggtaaaccg ctggtttcta ccagtgccaa
    24061 cttgagtgga ttgccacctt gtcgaacagt agacgaagtt cgcgcacaat ttggcgcggc
    24121 gttcccggtt gtgcctggtg aaacgggggg gcgtttaaat ccttcagaaa tccgcgatgc
    24181 cctgacgggt gaactgtttc gacaggggta acataatgga aacctatgct gtttttggta
    24241 atccgatagc ccacagcaaa tcgccattca ttcatcagca atttgctcag caactgaata
    24301 ttgaacatcc ctatgggcgc gtgttggcac ccatcaatga tttcgtcaac acactgaacg
    24361 ctttctttag tgctggtggt aaaggtgcga atgtgacggt gccttttaaa gaagaggctt
    24421 ttgccagagc ggatgagctt actgaacggg cagcgttggc tggagcagtt aataccctca
    24481 agcgtttaga agatggacgc ctgctggggg acaataccga tggtataggc ttgttaagcg
    24541 atctggaacg tctgtctttt atccgtcctg gtttacgtat tctgctcatc ggcgctggtg
    24601 gagcatctcg cggcgtacta ctgccacttc tttccctgga ctgtgcggtg acaatcacta
    24661 atcgaacggt atcccgcgcg gaagagttgg ctaaattgtt tgcgcacact ggcagtattc
    24721 agacgttgag tatgggcgaa ctggaaggtc atgagtttga tctcattatt aatgccacat
    24781 ccagtggcat cagtggtgat attccggcga tcccgtcatc gctcattcat ccaggcattt
    24841 attgctatga catgttctat cagaaaggaa aaattccttt tttggcatgg tgtgagcagc
    24901 gaggctcaaa gcgtactgct gatggtttag gaatgctggt ggcacaggcg gctcatgcct
    24961 tccttctttg gcatggcgtt ctgcctgatg tagaaccagt tataaagcaa ttgcaggagg
    25021 aattgtccgc gtgaatcagg ccatccagtt tccggataga gaagagtggg tcgagaataa
    25081 aaaatgtgta tgttttcccg ctctcgtgaa tggcatgcaa ctgacatgtg cgatctctgg
    25141 cgatagcctg gcgtatcgct ttactggaga tacgccagaa cagtggttag cgagttttcg
    25201 tcagcatcgc tgggacctgg aagaagaagc ggaaaactta attcaggaac aaagtgaaga
    25261 tgatcaaggt tgggtctggt taccctgatc caggtattcg tctttccatt tcacgtaatt
    25321 attcgcggaa taacgtaacc cagccttctc ttcatcactt aacgggcgga tctgtttgac
    25381 gggactaccg agatacagat atccgctctc cagccgttta ttttgtggga ccaggctacc
    25441 cgcaccaatc atcacatcat cttctactat tgcgccatca agtaaaattg agcccatccc
    25501 aaccaaaact cgattgccaa tggtgcagcc gtggagcatc accttgtgac caacagtgac
    25561 atcttcgcca atgattaatg ggttgccatc tgggttgtac gaagatttat gagtgacatg
    25621 caacatactg ccatcctgga tattggtgcg tgctccgatc tgtacataat gtacatctcc
    25681 acgaatcaca acgagcggcc agatccccac atcatcagcc agacgaacgt caccaatcac
    25741 gacactgcta tcgtcgatca ttacgcgctg accgatttgt ggaaaaagat cgcggtatgg
    25801 gcgtaaaaca tcagacatac ttacctcagc aataaatgat ttactaatga ctttgggggc
    25861 attattggcc ttgtgcaagt cttttagtat gcaaaaaagc accgttttgt gtgcgaatgc
    25921 agcaaaaagg gtgaaaaaac aacaaacaga aaaaaagatc aaaaaagtac ttgtgcaaaa
    25981 aattgggatc cctataatgc gcctccgttg agacgacaac gtgaaacact tcacaagatg
    26041 gtcgtaacaa cgaagagaaa aaatcctgaa attcagggtt gactctgaaa gaggaaagcg
    26101 taatatacgc cacctcgcg
//
LOCUS       DAPGOF010000059        26077 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.059, whole genome shotgun sequence.
ACCESSION   DAPGOF010000059 DAPGOF010000000
VERSION     DAPGOF010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26077
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.059"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..1786)
                     /gene="espL1"
                     /locus_tag="RHV13_002122"
     CDS             complement(<1..1786)
                     /gene="espL1"
                     /locus_tag="RHV13_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HDV1241653.1"
                     /translation="MSQNDIIIRTHYKSPHRMHIDSDIPTPSSEPINQFARQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFTINFATSPIHAENILITLHYKKEII
                     SLLLETTPIKANHLRSILDYIEQEQLTAENRNHCMKLSKKIHREKTIQPTVNLNGSAF
                     FSQSPSDAIFCRHLSLQYALDSLGNGEGKVNLIKHYSSVESIQQHVPLVRDAEFRALL
                     RHPPAGSRVIASKDFGFALDIFFCRMMANNVSHMSAILYIDNHTLSVRLRIKQSAYGQ
                     LNYVVSVYDPNDTNVAVRGTHRTARGFLSLDKFISSGPDAQTWADRYVRNCAIAILPL
                     LPEGVPGAIFTGIATRMPFAPIHPSAMLLIMATGQTQQLITLFRQLHILPEKEIIEII
                     TAQNSVGTPALFLAMMNGHTDNVKIFMQEIQSLVDNHIIHEDNLVKLLQTKSANETPG
                     LYISMLYGFDEIIDIFLNALTTPIAQELLNKKLVMSILAMKIHDGEPGLYAAMENNHP
                     LCVTRFLSKINGIAFKYKLSKANIMDLLKGATAQGTPALYIAMSKGNEDVVLSYISTL
                     GAFAKKHSFSQHQLFTLLAAKNHDNMSAV"
     gene            2309..4237
                     /gene="thrS"
                     /locus_tag="RHV13_002123"
     CDS             2309..4237
                     /gene="thrS"
                     /locus_tag="RHV13_002123"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDV1241654.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            4241..4783
                     /gene="infC"
                     /locus_tag="RHV13_002124"
     CDS             4241..4783
                     /gene="infC"
                     /locus_tag="RHV13_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDV1241655.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            4880..5077
                     /gene="rpmI"
                     /locus_tag="RHV13_002125"
     CDS             4880..5077
                     /gene="rpmI"
                     /locus_tag="RHV13_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDV1241656.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            5130..5486
                     /gene="rplT"
                     /locus_tag="RHV13_002126"
     CDS             5130..5486
                     /gene="rplT"
                     /locus_tag="RHV13_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDV1241657.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    5603..5727
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            5609..5653
                     /gene="pheM"
                     /locus_tag="RHV13_002127"
     CDS             5609..5653
                     /gene="pheM"
                     /locus_tag="RHV13_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HDV1241658.1"
                     /translation="MNAAIFRFFFYFST"
     gene            5936..6919
                     /gene="pheS"
                     /locus_tag="RHV13_002128"
     CDS             5936..6919
                     /gene="pheS"
                     /locus_tag="RHV13_002128"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDV1241659.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            6934..9321
                     /gene="pheT"
                     /locus_tag="RHV13_002129"
     CDS             6934..9321
                     /gene="pheT"
                     /locus_tag="RHV13_002129"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDV1241660.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQYKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVAEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            9326..9625
                     /gene="ihfA"
                     /locus_tag="RHV13_002130"
     CDS             9326..9625
                     /gene="ihfA"
                     /locus_tag="RHV13_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HDV1241661.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            9726..10706
                     /gene="btuC"
                     /locus_tag="RHV13_002131"
     CDS             9726..10706
                     /gene="btuC"
                     /locus_tag="RHV13_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HDV1241662.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRALL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            10769..11320
                     /gene="btuE"
                     /locus_tag="RHV13_002132"
     CDS             10769..11320
                     /gene="btuE"
                     /locus_tag="RHV13_002132"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HDV1241663.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKFAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            11320..12069
                     /gene="btuD"
                     /locus_tag="RHV13_002133"
     CDS             11320..12069
                     /gene="btuD"
                     /locus_tag="RHV13_002133"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HDV1241664.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEIRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLDDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSTLCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            12147..12611
                     /locus_tag="RHV13_002134"
     CDS             12147..12611
                     /locus_tag="RHV13_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDV1241665.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            12858..13571
                     /gene="ydiV"
                     /locus_tag="RHV13_002135"
     CDS             12858..13571
                     /gene="ydiV"
                     /locus_tag="RHV13_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HDV1241666.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGVDTAEILAQITPFDFHAL
                     LGCLWPAVPINQITTLVQR"
     gene            13634..15070
                     /locus_tag="RHV13_002136"
     CDS             13634..15070
                     /locus_tag="RHV13_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HDV1241667.1"
                     /translation="MTLSFITRWRDELPATYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQQLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYCLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLNDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(15074..15265)
                     /gene="hemP"
                     /locus_tag="RHV13_002137"
     CDS             complement(15074..15265)
                     /gene="hemP"
                     /locus_tag="RHV13_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HDV1241668.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(15397..16443)
                     /gene="aroH"
                     /locus_tag="RHV13_002138"
     CDS             complement(15397..16443)
                     /gene="aroH"
                     /locus_tag="RHV13_002138"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HDV1241669.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGSQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(16600..17433)
                     /gene="ppsR"
                     /locus_tag="RHV13_002139"
     CDS             complement(16600..17433)
                     /gene="ppsR"
                     /locus_tag="RHV13_002139"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HDV1241670.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            17766..20144
                     /gene="ppsA"
                     /locus_tag="RHV13_002140"
     CDS             17766..20144
                     /gene="ppsA"
                     /locus_tag="RHV13_002140"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HDV1241671.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPSEFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(20201..21847)
                     /gene="fadK"
                     /locus_tag="RHV13_002141"
     CDS             complement(20201..21847)
                     /gene="fadK"
                     /locus_tag="RHV13_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HDV1241672.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(21904..22197)
                     /locus_tag="RHV13_002142"
     CDS             complement(21904..22197)
                     /locus_tag="RHV13_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HDV1241673.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(22194..23483)
                     /locus_tag="RHV13_002143"
     CDS             complement(22194..23483)
                     /locus_tag="RHV13_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDV1241674.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(23539..24477)
                     /locus_tag="RHV13_002144"
     CDS             complement(23539..24477)
                     /locus_tag="RHV13_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HDV1241675.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(24497..25261)
                     /locus_tag="RHV13_002145"
     CDS             complement(24497..25261)
                     /locus_tag="RHV13_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HDV1241676.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIIIDNDSPEAIAELAEHLKKALN"
     gene            25577..>26077
                     /locus_tag="RHV13_002146"
     CDS             25577..>26077
                     /locus_tag="RHV13_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDV1241677.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKN"
BASE COUNT         6496 a         6214 c         6801 g         6566 t
ORIGIN      
        1 gaacagctga catgttgtca tgatttttag cagccaacag tgtaaataac tgatgttgac
       61 taaaagaatg tttttttgca aaagcaccca gcgtcgatat ataagataac acgacgtctt
      121 cattaccctt gctcatggcg atgtataatg caggggttcc ctgtgctgta gcgcctttta
      181 ataaatccat gatgttagct ttgctcaact tgtatttaaa ggcgatgcca ttaattttag
      241 agaggaaccg tgtgacacac aaagggtgat tattttccat tgcggcgtat aatcctggct
      301 caccatcgtg tattttcatg gctaaaatac tcatcaccag ttttttgttt aaaagctctt
      361 gtgctatagg agtggttaat gcattcagaa agatatcgat tatttcatcg aatccataca
      421 acatggagat ataaagtcca ggtgtttcgt tagcactttt agtttgcaac aatttaacca
      481 gattatcttc atgaatgatg tgattatcta ccagtgactg aatttcttgc ataaatattt
      541 ttacgttgtc agtatgtccg ttcatcatag ccaaaaataa agcaggtgta ccaacgctat
      601 tctgcgcagt tattatttca atgatttctt tttcagggag tatgtgcaac tgtctgaata
      661 gtgtaataag ctgttgagtc tggcctgtgg ccattattaa caacattgcc gatggatgta
      721 taggggcaaa tggcattcgt gtcgcgatac ccgtgaaaat agcccctgga actccctcag
      781 gtaatagggg cagaatagca attgcacagt tgcgaacata cctatcagcc caggtctgag
      841 catcgggacc tgaactgatg aacttatcaa gcgagagaaa gccccgcgct gtcctgtggg
      901 tgcctctgac ggcaacgttg gtatcgttcg ggtcgtaaac ggacactaca tagtttaatt
      961 gcccatacgc tgactgcttt attcgtagcc ttactgacaa agtatgattg tctatatata
     1021 aaatcgcaga catatgactg acattgtttg ccatcattcg acagaagaaa atatctaaag
     1081 cgaagccaaa atccttactc gcgataacgc gactccctgc aggaggatgg cgaagtaatg
     1141 ctctgaactc cgcgtcccgg actaagggga catgctgttg tatggattca acggaggagt
     1201 aatgtttaat caggttgact ttgccttctc catttcccag tgaatcaagg gcgtattgca
     1261 atgacagatg gcgacaaaaa atagcgtcag aaggagattg cgaaaaaaat gcactaccat
     1321 tgagatttac tgttggttgt atagtttttt ctctatggat ttttttagac agtttcatac
     1381 aatggttacg attttcggca gttaactgtt cctgttcaat ataatccagt atgcttcgca
     1441 aatggttagc tttaataggc gtggtttcca gtaataagga aatgatttct tttttatagt
     1501 gtaatgtaat gagtatattt tctgcatgga tgggtgaagt ggcaaagtta atggtaaaaa
     1561 gggcagtgga ataacaattt tgtgatattt ttcgacagtt tgcggtaaat tcctgagtaa
     1621 cacagtatga cagcatcgaa cttaagtcag aggtatcaag tagggtgatg agttggcgcg
     1681 caaattgatt aataggctct gatgaaggcg ttggtatgtc gctatcgatg tgcattctat
     1741 gaggagactt ataatgagtt ctgataataa tgtcgttttg actcataatc tattcgcctt
     1801 atatatgtct ggaggttttg aggatgatga ttaattatta tcgccagaac gaaatgtgca
     1861 cgaaaaattg ctataagaat tgtgtcactt ttatcattgt gatatgacca gttcgtcatt
     1921 aatctcatga tcgtttaaat agtaccgcca aatcatgcaa cgaactttgc tggattcagt
     1981 ttatgctgct gtaaatccgc tcgcagtaaa cctttcagac gctccgtgat gttatcagtt
     2041 gttctttaag cgttttgctg gtgtactcac tacaaacgaa ttgcgaatcg aatcaatgtg
     2101 aaacggaaag gtacaatctc ccttttttta gtgttaacat cgctcaaccg ggtggaaggt
     2161 gagaggttaa tgtctttcat ttgtgtacct gattgtgaac caatttagca tttgttggct
     2221 agatggtttc gcaatgaact gttaataaac aaatttttct ttgtatgtga tctttcgtgt
     2281 gggtcaccac tgcaaataag gatataaaat gcctgttata actcttcctg atggcagcca
     2341 acgccattac gatcacgctg taagccccat ggatgttgcg ctggacattg gtccaggtct
     2401 ggcgaaagcc tgtatcgcag ggcgcgttaa tggcgaactg gttgatgctt gcgatctgat
     2461 tgaaaacgac gcacaactgt cgatcattac cgcgaaagac gaagaaggtc tggagatcat
     2521 tcgtcactcc tgtgcgcacc tgttagggca cgcgattaaa caactttggc cgcataccaa
     2581 aatggcaatc ggcccggtta ttgacaacgg tttttattac gacgttgatc ttgaccgcac
     2641 gttaacccag gaagatgtcg aagcactcga gaagcggatg catgagcttg ctgagaaaaa
     2701 ctacgacgtc attaagaaga aagtcagctg gcacgaagcg cgtgaaactt tcgccaaccg
     2761 tggggagagc tacaaagtct ccattcttga cgaaaacatc gcccatgatg acaagccagg
     2821 tctgtacttc catgaagaat atgtcgatat gtgccgcggt ccgcacgtac cgaacatgcg
     2881 tttctgccat catttcaaac taatgaaaac ggcaggggct tactggcgtg gcgacagcaa
     2941 caacaaaatg ttgcaacgta tttacggtac ggcgtgggca gacaaaaaag cacttaacgc
     3001 ttacctgcag cgcctggaag aagccgcgaa acgcgaccac cgtaaaatcg gtaaacagct
     3061 cgacctgtac catatgcagg aagaagcgcc gggtatggta ttctggcaca acgacggctg
     3121 gaccatcttc cgtgaactgg aagtgtttgt tcgttctaaa ctgaaagagt accagtatca
     3181 ggaagttaaa ggtccgttca tgatggaccg tgtcctgtgg gaaaaaaccg gtcactggga
     3241 caactacaaa gatgcaatgt tcaccacgtc ttctgagaac cgtgaatact gcattaagcc
     3301 gatgaactgc ccgggtcacg tacaaatttt caaccagggg ctgaagtcgt atcgcgatct
     3361 gccgctgcgt atggccgagt ttggtagctg ccaccgtaat gagccgtcag gttcgctgca
     3421 tggcctgatg cgcgtgcgtg gatttaccca ggatgacgcg catatcttct gtactgaaga
     3481 acaaattcgc gatgaagtta acggatgtat ccgtttagtc tatgatatgt acagcacttt
     3541 tggcttcgag aagatcgtcg tcaaactctc cactcgtcct gaaaaacgta ttggcagcga
     3601 cgaaatgtgg gatcgtgctg aggcggacct ggcggttgcg ctggaagaaa acaacatccc
     3661 gtttgaatat caactgggtg aaggcgcttt ctacggtccg aaaattgaat ttaccctgta
     3721 tgactgcctc gatcgtgcat ggcagtgcgg tacagtacag ctggacttct ccttgccgtc
     3781 tcgtctgagc gcttcttatg taggcgaaga caatgaacgt aaagtaccgg taatgattca
     3841 ccgcgcaatt ctggggtcga tggaacgttt catcggtatc ctgaccgaag agttcgctgg
     3901 tttcttcccg acctggcttg cgccggttca ggttgttatc atgaatatta ccgattcaca
     3961 gtctgaatac gttaacgaat tgacgcaaaa actatcaaat gcgggcattc gtgttaaagc
     4021 agacttgaga aatgagaaga ttggctttaa aatccgcgag cacactttgc gtcgcgtccc
     4081 atatatgctg gtctgtggtg ataaagaggt ggaatcaggc aaagttgccg ttcgcacccg
     4141 ccgtggtaaa gacctgggaa gcatggacgt aaatgaagtg atcgagaagc tgcaacaaga
     4201 gattcgcagc cgcagtctta aacaattgga ggaataaggt attaaaggcg gaaaacgagt
     4261 tcaaacggcg cgccctaacc gtatcaatgg cgaaattcgc gcccaggaag ttcgcttaac
     4321 aggtctggaa ggcgagcagc ttggtattgt gagtctgaga gaagctctgg agaaagcaga
     4381 agaagccgga gtagacttag tcgagatcag ccctaacgcc gagccgccgg tttgtcgtat
     4441 aatggattac ggcaaattcc tctatgaaaa gagcaagtct tctaaggaac agaagaaaaa
     4501 gcaaaaagtt atccaggtta aggaaattaa attccgtcct ggtacagatg aaggcgacta
     4561 tcaggtaaaa ctccgcagcc tgattcgctt tctcgaagag ggtgataaag ccaaaatcac
     4621 gctgcgtttc cgcggtcgtg agatggcgca ccagcaaatc ggtatggaag tgcttaatcg
     4681 cgtgaaagac gatttgcaag aactggcagt ggtcgaatcc ttcccaacga agatcgaagg
     4741 ccgccagatg atcatggtgc tcgctcctaa gaagaaacag taaggccttc aagcagcact
     4801 gtctgtggag ccttcgggct tcacaggctt tgttcgcctt tgtttcgttt attaacaatg
     4861 cgaagtggaa gttattaaaa tgccaaaaat taagaccgta cgcggtgctg ctaagcgctt
     4921 caaaaaaacc ggtaaaggtg gttttaagca caagcacgct aacctgcgtc acattctgac
     4981 caaaaaagcg accaaacgta aacgtcacct gcgtccgaaa gccatggttt ccaaaggcga
     5041 tctgggcctg gtaatcgcgt gcctgccgta cgcataagcc gttaacgttt ttaacttttt
     5101 aattagaata tagatacagg agagcacata tggctcgcgt aaaacgtggt gttattgcac
     5161 gcgcacgtca caagaaaatt ttgaaacaag ctaaaggcta ctacggtgcg cgttctcgcg
     5221 tataccgcgt tgccttccag gctgttatca aagctggtca gtatgcttac cgtgaccgtc
     5281 gtcaacgtaa gcgtcagttc cgtcaactgt ggattgcgcg tatcaacgca gcagcacgtc
     5341 agaacggtat ttcttacagc aaattcatca atggcctgaa aaaagcctct gttgaaatcg
     5401 accgtaagat cctggctgat atcgcagtat tcgacaaagt agcgttcacc gctctggttg
     5461 aaaaagcgaa agcagctctg gcataagcca gttgaaagag ggagctagtc tccctctttt
     5521 cgtttcaacg ccatcaaaac attgactttt atcgccgtag ccttttcaat aaaggtcttt
     5581 tgaagagtac caaaaggtaa cgcaagcaat gaatgctgct attttccgct tcttttttta
     5641 ctttagcacc tgaatccagg aggctagcgc gtgagaagag aaacggaaaa cagcgcctga
     5701 aagcctccca gtggaggctt tttttgtatg cgcgttttga aaaataaaaa gccagtctat
     5761 caggcgtaat tgtcgcagcc agtctggata cggccagtgc ggagaaaccg gagcgtactt
     5821 aagtacgtga ggatttcgag cactgcccgg gaccaaaatg gcaagtaaaa tagcctgatg
     5881 ggataggctc taagtccaac gaaccagtgt caccactgac acaatgagga aaaccatgtc
     5941 acatctcgca gaactggttg ccagtgcgaa ggcggccatt agccaggcgt cagatgttgc
     6001 cgcgttagat aatgtgcgcg tcgaatattt gggtaaaaaa gggcacttaa cccttcagat
     6061 gacgaccctg cgtgagctgc cgccagaaga gcgtccggca gctggtgcgg ttatcaacga
     6121 agcgaaagag caggttcagc aggcgctgaa tgcgcgtaaa gcggaactgg aaagcgctgc
     6181 actgaatgcg cgtctggcgg cggaaacgat tgatgtctct ctgccaggtc gtcgcattga
     6241 aaacggcggt ctgcatccgg ttacccgtac catcgaccgt atcgaaagtt tcttcggtga
     6301 gcttggcttt accgtggcaa ccgggccgga aatcgaagac gattatcata acttcgatgc
     6361 tctgaacatt cctggtcacc acccggcgcg cgctgaccac gacactttct ggtttgacgc
     6421 tacccgcctg ctgcgtaccc agacctctgg cgtacagatc cgcaccatga aagcccagca
     6481 gccaccgatt cgtatcatcg cgcctggccg tgtttatcgt aacgactacg accagactca
     6541 cacgccgatg ttccatcaga tggaaggtct gattgttgat accaacatca gctttaccaa
     6601 cctgaaaggc acgctgcacg acttcctgcg taacttcttt gaggaagatt tgcagattcg
     6661 cttccgtcct tcctacttcc cgtttaccga accttctgca gaagtggatg tcatgggtaa
     6721 aaacggtaaa tggctggaag tactgggctg cgggatggtg catccgaacg tgctgcgtaa
     6781 cgttggcatc gacccggaag tttactctgg tttcgccttc gggatgggga tggagcgtct
     6841 gactatgttg cgttacggcg tcaccgacct gcgttcattc ttcgaaaacg atctgcgttt
     6901 cctcaaacag tttaaataag gcaggaatag attatgaaat tcagtgaact gtggttacgc
     6961 gaatgggtga acccggcgat tgatagcgat gcgctggcga atcaaatcac tatggcgggc
     7021 ctggaagttg acggtgtaga accggttgcc ggtagcttcc acggcgtggt cgttggtgaa
     7081 gtggttgagt gtgcgcagca tccgaacgct gacaaactgc gtgtgacaaa agtgaatgtc
     7141 ggcggcgatc gcctgctgga catcgtctgc ggtgcgccaa actgccgtca gggcctgcgt
     7201 gtggcggtag cgaccattgg tgctgttctg ccgggtgatt tcaaaattaa agcggcgaaa
     7261 ctgcgtggcg aaccgtctga agggatgctg tgctccttct ctgaactggg aatttctgac
     7321 gatcacagcg gcattatcga actgcctgca gatgcgccga ttggcaccga tatccgcgaa
     7381 tacctgaaac tcgatgacaa caccatcgaa atcagcgtaa cgccaaaccg tgccgactgt
     7441 ttaggtatca ttggtgttgc gcgtgacgtt gccgtgctga atcagctgcc gctggttgaa
     7501 ccggaaatcg ttccggttgg tgcgaccatc gacgacacgc tgccgattac agtcgaagcg
     7561 ccggaagcct gcccgcgtta tcttggccgt gtggtaaaag gcattaacgt taaagcgcca
     7621 actccgctgt ggatgaaaga aaaactgcgt cgttgcggga tccgttctat cgatgcagtt
     7681 gttgacgtca ccaactatgt gctgctcgaa ctgggccagc cgatgcacgc tttcgataaa
     7741 gatcgcattg aaggcggcat tgtggtgcgg atggcgaaag agggtgaaac gctggtgctg
     7801 cttgatggca ctgaagcgaa gctgaatgct gacaccctgg ttatcgccga ccacaacaag
     7861 gcgctggcga tgggcggcat ttttggtggc gaacactctg gcgtgaatga cgaaacgcaa
     7921 aacgtcctgc tggaatgcgc tttcttcagc ccgctgtcta tcaccggtcg tgctcgtcgt
     7981 catggcctgc atactgatgc gtctcaccgt tatgagcgtg gcgttgatcc ggcactgcag
     8041 tacaaagcga tggaacgtgc gacccgtttg ctgatcgaca tctgcggtgg tgaggctggc
     8101 ccggtaattg atatcaccaa cgaagcaacg ctgccgaagc gtgcaaccat cactctacgt
     8161 cgtagcaaac tggatcgcct gatcggccat catattgcgg atgagcaggt aactgacatt
     8221 ctgcgtcgtc tcggctgcga agtggccgaa ggcaaagacg agtggcaggc agttgcgccg
     8281 agctggcgtt tcgacatgga gattgaagaa gatctggtcg aagaagtcgc gcgtgtttac
     8341 ggctacaaca acatcccgga tgagccggta caggcaagcc tgattatggg tactcaccgt
     8401 gaagctgacc tgtcgctcaa gcgcgtgaaa acgctgctca acgacaaagg ctatcaggaa
     8461 gtgatcacct acagcttcgt tgatccgaaa gtgcagcaga tgatccatcc aggcgttgaa
     8521 gccttactgc tgccaagccc gatctctgtt gaaatgtcag caatgcgtct ttctctgtgg
     8581 accggcctgc tggcaaccgt ggtgtacaac cagaaccgtc agcagaaccg tgtgcgcatt
     8641 ttcgaaagcg gtctgcgttt cgtaccagat actcaggcac cgttgggcat tcgtcaggat
     8701 ctgatgttag ccggtgtgat ttgcggtaac cgttacgaag agcactggaa cctggcaaaa
     8761 gagaccgttg atttctatga tttgaaaggc gatcttgaat ccgttctcga cctgaccggt
     8821 aaactgaatg aggttgagtt ccgtgcagaa gcgaatccgg cactgcatcc ggggcaatcc
     8881 gcagcgattt atctgaaagg tgaacgtatt ggttttgttg gagttgttca tcctgaactg
     8941 gaacgtaaac tggatcttaa cggtcgcact ctggtgttcg aactggagtg gaacaagctc
     9001 gcagaccgcg tggtgcctca ggcgcgcgag atttctcgct tcccggcgaa ccgtcgtgac
     9061 atcgcggtgg tggtcgcaga aaacgttccc gcagcggata ttttatccga atgtaagaaa
     9121 gttggcgtaa atcaggtagt tggcgtaaac ttatttgacg tgtaccgcgg taagggtgtt
     9181 gcggaggggt ataagagcct cgccataagc ctgatcctgc aagataccag ccgtacactc
     9241 gaagaagagg agattgccgc taccgtcgcc aaatgtgtag aggcattaaa agagcgattc
     9301 caggcatcat tgagggattg aacctatggc gcttacaaaa gctgaaatgt cagaatatct
     9361 gtttgataag cttgggctta gcaagcggga tgccaaagaa ctggttgaac tgtttttcga
     9421 agagatccgt cgcgctctgg aaaacggcga acaggtgaaa ctctctggtt ttggtaactt
     9481 cgatctgcgt gataagaatc aacgcccggg acgtaacccg aaaacgggcg aggatattcc
     9541 cattacagca cggcgcgtgg tgaccttcag acccgggcag aagttaaaaa gccgggtcga
     9601 aaacgcttcg cccaaagacg agtaatctga tctaactaaa aaggccgctc tgcggccttt
     9661 tttcttttca ctgtcgaaga gtcaccgtaa aatcaacgcc atgacacttc agcagaacgg
     9721 ataccatgct gacacttgcc cgccaacaac agcgacaaaa tattcgctgg ttattatgcc
     9781 tgtcagtttt gatgctgctg gcgcttctct taagcctttg cgccggtgaa caatggatct
     9841 cgccaggtga ctggtttact cctcgtggcg aactgttcgt ctggcaaatt cgcctgccac
     9901 gtacgctggc tgtattgctg gttggtgcgg cgctggctat atccggcgct gtaatgcagg
     9961 cgttgtttga aaatcctctg gcagaacctg gactacttgg cgtctctaac ggcgcaggcg
    10021 tggggcttat cgccgcggta ttgcttgggc aagggcaact ccccaactgg gcgctagggc
    10081 tgtgtgcgat tgctggcgcg cttatcatca ctttaatact cttacgtttc gcccgtcgtc
    10141 atctttcgac cagtcggtta ttgctggctg gcgttgcatt agggattatc tgtagcgcac
    10201 taatgacgtg ggctatctac ttttccacct ctgttgattt acgtcagctg atgtactgga
    10261 tgatgggcgg ttttggcggc gtagactggc ggcaaagctg gctgatgctg gcattgatcc
    10321 ccgtgttgtt gtggatctgt tgtcagtcca ggccgatgaa tatgttagca cttggcgaga
    10381 tctcggcgcg gcaactgggt ttacccctgt ggttctggcg caatgtgctg gtggcagcga
    10441 ccggctggat ggttggcgtc agtgtggcgc tggcgggtgc tatcggcttt attggtctgg
    10501 tgatcccaca tattcttcgg ttgtgtggtt taaccgatca tcgcgcatta cttcccggct
    10561 gcgcgctggc aggggcgagc gcattgctgc tggccgatat tgtagcgcgc ctggcattag
    10621 ctgccgcaga gctgcctatt ggcgtggtca ccgcaacgtt gggtgcgccg gtgtttatct
    10681 ggttattgtt aaaagcagga cgttagccac aaaaagacgg tctatgatta aaagctaaga
    10741 tttttactga ccacacccag gagaaacgat gcaagattcc attctgacga ccgtagtgaa
    10801 agatatcgac ggtgaagtga ccacgctgga gaagttcgcc ggtaatgtgc tgttgattgt
    10861 caatgtcgcc tcaaagtgtg gcttaacgcc gcaatatgag cagttggaga atattcagaa
    10921 agcctgggcc gatcgaggtt ttgtggtgct gggattcccg tgcaaccagt ttctggaaca
    10981 agaaccgggc agcgatgaag agattaaaac ttactgtacc accacatggg gggtgacgtt
    11041 cccgatgttc agtaagattg aagttaacgg cgaaggacgc catccgctgt atcaaaaatt
    11101 gattgccgca gcgccgaccg cagtcgcgcc ggaagagagc ggattctatg cccgtatggt
    11161 cagcaaaggt cgtgcaccgc tgtaccctga tgatatttta tggaattttg agaaattttt
    11221 agttggcagg gacggattgg tcatccagcg tttttccccg gatatgacgc cggaagatcc
    11281 cattgtgatg gaaagcatta aactggcgtt ggcaaaataa tgtctattgt gatgcagtta
    11341 caggatgttg cggaatctac ccgcctgggg ccgctttctg gcgagattcg ggctggggag
    11401 atcctgcacc tggtggggcc gaatggcgcg ggtaagagta ccttactggc acgaatggcc
    11461 ggaatgacca gcggtaaggg aagcattcag ttcgcggggc aaccactgga agcatggtcc
    11521 gcaacaaaac tcgcgctgca tcgcgcctat ctttcacaac agcagacgcc gccgtttgca
    11581 atgccggtct ggcactacct gacactgcat cagcacgata aaacgcgtac cgaactactg
    11641 gatgatgtcg caggggcgct ggctcttgat gacaaactcg gtcgtagcac caatcaactt
    11701 tccggcggtg aatggcaacg cgtacgtctt gctgcggtgg tgttgcaaat cacaccacaa
    11761 gccaatcccg caggccaatt gctgcttctt gatgagccga tgaacagtct tgatgttgcg
    11821 caacaaagtg cgttagacaa aattctgagc acgctgtgtc agcaaggact ggcgattgtg
    11881 atgagcagtc acgatctcaa ccacacgttg cgtcatgcgc atcgggcgtg gttgctaaaa
    11941 ggtggaaaaa tgctggccag tggacgcaga gaagaggtgc tcacgccgcc aaatctggcg
    12001 caggcctatg ggatgaattt tcgccgtctg gatatcgaag gtcacagaat gctgatttcg
    12061 accatctgat ttcattagta tcttgaaaat cgggtaatta cgacgctaaa ttaatgcaag
    12121 aataaaaaac agaggaacca tacggaatgc gtttctgcct tattttgatc acagcactgt
    12181 ttctggccgg gtgtagccac cataaagcac cgccgccaaa tgccagactt tctgattcga
    12241 ttaccgttat tgccggtttg aacgaccagc tacaaagctg gcatggcacg ccgtatcgtt
    12301 atggtggcat gacgcggcgc ggtgtggact gttcgggatt tgtggttgtg acgatgcgcg
    12361 atcgtttcga tttgcagctg ccccgagaaa ccaaagaaca agcctctatc ggcacgcaaa
    12421 ttgataaaga cgagttgctg cctggtgacc tggtcttttt caaaacgggt tccggacaaa
    12481 atggtttgca tgtaggtatt tacgatacca acaaccaatt tatccacgcc tctaccagca
    12541 agggagtgat gcgttcctca cttgataatg tctattggca gaaaaatttc tggcaggcga
    12601 gacgaatcta gtatgtcgaa aattggacgg cgatgataca tcgccgtcca tttactccag
    12661 aatacagtag tattttgcag acaaaaattc cgtaaatgga ttattcgttt ataatcagga
    12721 taatctaaaa aaaggacccc tgaaaaagtg gttaacatgg aacaaatgaa agttactcct
    12781 tatgtaattg ttatgtctta tggcctcatc ccttttagcc ggatactgaa aaacatcctt
    12841 cgagagggac ggttaccatg aagatttttt tggagaatct ttatcattct gattgctatt
    12901 tcctgccgat aagagataac cagcaagacc ttgtcggcgt tgagttaatt acccatttct
    12961 ccagtgaaga tggcacggtg cgtatcccga cgagtcgggt tatagcgcaa cttaccgagg
    13021 agcaacattg gcaattattt tcagagcagt tggaattact aaaatcatgc caacattttt
    13081 ttattcaaca taaactcttt gcctggctta atttaacgcc acaagtggct acattattat
    13141 tagagaggga taattacgca ggcgaactat taaaatatcc gtttattgaa ctgttgatta
    13201 atgagaatta cccgcatctc aatgaaggaa aagataatcg cgatcttttg tccttatcgc
    13261 aaatgtatcc ccttgtgtta ggtaatttgg gagcaggcaa tagcacaatg aaagcggttt
    13321 ttgatggtct tttcacccgc gtcatgctgg ataaaagttt tattcagcaa caaatcacgc
    13381 atcgctcctt cgaaccgttt attcgtgcga tccaggcgca aatttcccct tgttgcaatt
    13441 gcatcatcgc ggggggcgtt gatacggcag aaatattggc acagattacc cccttcgatt
    13501 tccatgctct cctgggatgc ctgtggcctg ccgttcctat aaatcagata acgacgttgg
    13561 ttcagcgata accctcctgt ttgctggtgt ttaagacgag agtaaccgtc tacactatca
    13621 aacaggagga tctatgaccc tgtcttttat tacccgctgg cgcgatgaat tgccagcaac
    13681 ctatacagca ctttccccta cgcctttaaa taatgcccgg ctgatttggc ataataccga
    13741 actcgctaac acgctgagta ttccatcgtc gctgtttaaa aatggcgcag gtgtctgggg
    13801 cggcgaaact ctactgcctg gtatgtcacc actggcgcag gtttacagtg gtcatcagtt
    13861 cggcgtctgg gcgggccaac tgggtgatgg gcgcggcatt ttacttggcg aacaacagct
    13921 tgctgatggc actacaatgg actggcatct gaaaggtgct ggcctgacgc cttattcgcg
    13981 aatgggtgat ggacgggcgg ttttacgttc gacgatacga gaaagtctcg ccagtgaggc
    14041 gatgcattat ctgggcattc cgacgacccg cgcgttaagt atcgtcacca gcgattcgcc
    14101 agtgtatcgg gaaacggtgg agccaggcgc gatgctgatg cgtgtggcac caagtcatct
    14161 gcgctttggt catttcgaac atttttacta tcgccgcgag ccggaaaagg ttcgtcagtt
    14221 ggctgacttt gccattcgtc attactggtc acatcttgaa gatgatgagg acaaatactg
    14281 tctctggttt aacgatgttg tcgcacgtac cgcatcgtta attgcccaat ggcagacggt
    14341 cggctttgca catggggtga tgaataccga caacatgtcg ctgctgggac tgacgcttga
    14401 ttacgggccg tttggttttc ttaatgatta cgaacccggt tttatttgta atcactcgga
    14461 tcatcaaggg cgttacagct ttgacaatca acctgctgtc gcgttgtgga atttacagcg
    14521 tctggcgcag acattgtcac catttgttgc cgtagatgcc ctgaatgagg ccctggacag
    14581 ctatcagcag gttttgttga cgcattatgg acaacggatg cggcagaaac tgggcttcat
    14641 gacggagcaa aaagaggata acgcgctact gaatgaatta ttcagtctga tggcgcgaga
    14701 gcgcagcgat tatacccgca catttcgcat gctgagtctg accgagcagc acagcgcggc
    14761 gtcaccgcta cgtgatgagt ttattgatcg tgcggcattt gatgactggt ttgcccgtta
    14821 tcgagggcgt ttgcaacaag acgaggttag cgatagtgag cgtcagcaac tgatgcaaag
    14881 cgtgaaccca gctctggtat tgcgcaactg gttggcgcaa cgggcgattg aagcggcaga
    14941 aaagggtgat atgacggaat tgcaccgcct gcatgaggcg ttgcgaaatc ctttcagcga
    15001 cagagatgat gactatgtca gtcgtccacc tgactggggt aaacggctgg aagtcagctg
    15061 ctcgagttaa aggctacttg gtcaacagca gcttgccagc ctgagtttta cggagcagat
    15121 attcttgccc gtcatgatcg ataatcagtt tgccatccgg acctaacagt gtctggctgg
    15181 aaattcgccg aataggctgc ggggaagcgg tcttgtgatt ggcatccgtt tcaggcgtga
    15241 gttttctgct atccgtataa cgcataaaaa ttaataataa ttgatataac aatgattctt
    15301 attatcaata ttatggctga ccagagcaag cagtaatgtg ttgagaagac aacaagttgc
    15361 gcagaaatgc ccattccgtc aggaatgggc acgcattcag aagcgggtat ctaccgcaga
    15421 ggcgagtttt tcgaccaggc gttcggtatc ttcccagccc agacacgggt cggtaatgga
    15481 ttgaccgtaa gtgagcggct gactgccgac gattttttgc gttccttcgc gcaggaaact
    15541 ttccgccata attccagcaa tcgccgtaga gccattgcgg atttgctgac aaatatcctc
    15601 acaaacttct aactggcgac ggtgctgctt ctggcagtta ccgtggctga aatccaccac
    15661 cagatgttca ggtaaatcaa actcgtgcag cgtatcgcag gctgcggcga tatcatcggc
    15721 atgataattc ggttttttgc cgccacgcat aataatgtgg ccatacgggt tgccgctggt
    15781 ctgatagatg gtcatctgac catttttgtc tggcgagagg aacatatggc tggcgcgggc
    15841 tgcgcggata gcatccacag caatccgcgt gttgccatcg gtaccatttt taaaacctac
    15901 cggacaggag agtgccgaag ccatttcacg gtggatctga ctttcggtag tacgtgcgcc
    15961 aatcgcgccc caactgatta aatcagcaat aaactgaccg gtcaccatat cgaggaactc
    16021 agtcgcggtt gggacgccca gctcatttac ctgtaaaagt aatttgcgcg ccagctccag
    16081 accgtgattt acccgatagc taccgtttaa atctggatcg gagattagtc ctttccagcc
    16141 gacaacagtt cgtggttttt caaaataggt gcgcattacg atttccagcc gtgactggta
    16201 ctggttgcgc agcgactgca gacgggtggc gtactccatt gcagcggtga gatcgtggat
    16261 cgagcagggg ccaataatga ccaacagtcg cttatcttca ccattcagta ttttttcaat
    16321 tctgcggcgg gagtcggtga catgggtggc gacgccaggc gttacgggat accgtagcgc
    16381 gagttcggcg ggcgttacca ggctctcaat acgcgcagta cggagttcgt cagttctgtt
    16441 cattacaagt ctcagaaatt tgttgcttct acggcaggac tgccgggaag tgatgcgcat
    16501 cacgataaac caatcgctgc tgatttcaag tgcgaggtgt gtcacacctc gccgggtggt
    16561 tagcatgata acaaaaaaat aagctaatgc actagttctc tagtacattc ggcgactaag
    16621 gcccatgata tcgaggatct tggtggcaat ctcttctacc gaataattgg tactgttaat
    16681 ccacgggatc tgatttttac ggtacaaggc ttccacttcc gcgacttcca tcctgcactg
    16741 acgaagcgag gcatagcgac tgttctcccg acgttcctcg cgaatcgccg ccagacgttc
    16801 cgggtcgata gtcaggccga acaatttatg ctgaagcggt ttgagcgacg cgggtagcac
    16861 cagattatcc atatcgtcgg caataaaggg gtagtttgcc gcgcggatac caaactgcat
    16921 tgccagatac agactggtgg gggttttacc acagcgagaa acaccgagca ggatcacctg
    16981 agcctgatcc agattgcgca acgaaatgcc gtcatcgtgg gcgagggtgt aatcaatcgc
    17041 cgcaatgcgc gcatcatatt tattgagatt attagggtta aggccatggg tacgatgagc
    17101 aatcggcgtt ggatccagtt tcatctcttg ttgtagcggg gcaaccagcg cctgaacgat
    17161 atcctggcaa aagccttcac tttgcaagat gatggcgcga atctccggca acacgatgga
    17221 gtagaagacc agcgggcgca cgcctgtctg gtgataaatt gcgtcaatct gatccttcac
    17281 tgcccgtgca cggctctcat tttcgacaaa cggcagcgtg atgctgctga tagtgacggg
    17341 aaattgtgac attactgcgt gtcctaatac ctccgcagtt attgccgtac catcagaaat
    17401 ataaaaaacg tggcgatcaa cagcattatc cattttgttc ttcccgtgat gcagacataa
    17461 ttctatgaaa gcataaatta aaaacgcaca gaagcgtaga acgttatgtc tggtttataa
    17521 aatgaacctt caattttatt ttttatgaaa acagcatttc atttttatgg tttcgtttat
    17581 accgatggtt tatgtggaaa ttgtcgaaga gagcagattt gcgcaacgct gggatcagtc
    17641 ttaaaaagta aaaaaatata tttgcttgaa cgattcaccg tttttttcat ccggttaaat
    17701 atgcaaagat aaatgcgcag aaatgtgttt ctcaaaccgt tcatttatca caaaaggatt
    17761 gttcgatgtc caacaatggc tcgtcaccgc tggtgctttg gtataaccaa ctcggcatga
    17821 atgatgtaga cagggttggg ggcaaaaatg cctccctggg tgaaatgatt actaaccttt
    17881 ccggaatggg tgtttccgtt ccgaatggtt ttgccacaac cgccgacgcg tttaaccagt
    17941 ttctggacca aagcggcgta aaccagcgca tttatgaact gctggataaa acggatattg
    18001 acgatgttac tcagcttgcg aaagcgggcg cgcaaatccg ccagtggatt atcgacactc
    18061 ccttccagcc tgagctggaa aacgccatcc gcgaagccta tgcacagctt tccgccgatg
    18121 acgaaaacgc ctcttttgcg gtgcgctcct ccgccaccgc agaagatatg ccggacgctt
    18181 cttttgccgg tcagcaggaa accttcctca acgttcaggg ttttgacgcc gttctcgtgg
    18241 cagtgaaaca tgtatttgct tctctgttta acgatcgcgc catctcttat cgtgtgcacc
    18301 agggttacga tcaccgtggt gtggcgctct ccgccggtgt tcaacggatg gtgcgctctg
    18361 acctcgcatc ttctggcgtg atgttctcca ttgataccga atccggcttt gaccaggtgg
    18421 tgtttatcac ttccgcatgg ggccttggtg agatggtcgt gcagggtgcg gttaacccgg
    18481 atgagtttta cgtgcataaa ccgacactgg cggcgaatcg cccggctatc gtgcgccgca
    18541 ccatggggtc gaaaaaaatc cgcatggttt acgcgccgac ccaggagcac ggcaagcagg
    18601 ttaaaatcga agacgtaccg caggaacagc gtgacatctt ctcgctgacc aacgaagaag
    18661 tgcaggaact ggcaaaacag gccgtacaaa ttgagaaaca ctacggtcgc ccgatggata
    18721 ttgagtgggc gaaagatggc cacaccggta aactgttcat tgtgcaggcg cgtccggaaa
    18781 ccgtgcgctc acgcggtcag gtcatggagc gttatacgct gcattcacag ggtaagatta
    18841 tcgccgaagg ccgtgctatc ggtcatcgca tcggtgcggg tccggtgaaa gtcatccatg
    18901 atatcagcga aatgaaccgc atcgaacctg gtgacgtgct ggtcactgac atgaccgacc
    18961 cggactggga accgatcatg aagaaagcat ctgccatcgt caccaaccgt ggcggtcgta
    19021 cctgtcacgc ggcgatcatc gctcgtgaac ttggcattcc ggcggtagtg ggctgtggtg
    19081 atgcaacaga acggatgaaa gacggtgaga acgtcactgt ttcttgtgcc gaaggtgata
    19141 ccggttacgt ctatgcggag ttgctggaat ttagcgtgaa aagctccagc gtagaaacga
    19201 tgccggatct gccgttgaaa gtgatgatga acgtcggtaa cccggaccga gctttcgact
    19261 tcgcctgtct gccgaacgaa ggcgtgggac ttgcgcgtct ggaatttatc atcaaccgta
    19321 tgattggcgt ccacccacgc gcactgcttg agtttgacga tcaggaaccg cagttgcaaa
    19381 acgaaatccg cgagatgatg aaaggttttg attctccgag tgaattttac gttggtcgtc
    19441 tgactgaagg gatcgcgacg ctgggtgccg cgttttatcc gaagcgcgtc attgtccgtc
    19501 tctctgattt taaatcgaac gaatatgcca acctggtcgg tggtgagcgt tacgagccag
    19561 atgaagagaa cccgatgctc ggcttccgtg gcgcgggccg ctatgtttcc gacagcttcc
    19621 gcgactgttt cgcgctggag tgcgaagcag tgaaacgtgt gcgcaacgac atggggctga
    19681 ccaacgttga gatcatgatc ccgttcgtgc gtaccgtaga tcaggcgaaa gcggtggttg
    19741 aagaactggc gcgtcagggg ctgaagcgtg gcgagaacgg gctgaaaatc atcatgatgt
    19801 gtgaaatccc gtccaacgcc ttgctggccg agcagtttct cgaatatttc gacggcttct
    19861 caattggctc aaacgatatg acacagctgg cgctcggtct ggatcgtgac tcaggcgtgg
    19921 tgtctgaact gttcgatgag cgcaacgatg cggtgaaagc actgttgtcg atggcgattc
    19981 gtgccgcgaa gaaacagggc aaatatgtcg ggatttgcgg tcagggtccg tccgaccacg
    20041 aagactttgc cgcatggttg atggaagagg ggatcgatag cctgtctctg aacccggaca
    20101 ccgtggtgca aacctggtta agcctggccg aactgaagaa ataaaataaa tacccggcgg
    20161 cgttttgtcg ccgggtttat gtgatccccg aagatgaaac ttattcaatc tcttcacaga
    20221 catcctgagt taaccgccgc ataatatctt ttcttaacaa aaacttttgt attttacctg
    20281 aggcagtgcg cggtagtttt tcgattacca cgatatgttc aggatattta tattttgcga
    20341 cccgtttacg gctaaaaaaa gccaccacct cttccagcga taatgaatga tgcggcgctt
    20401 tcagcacgac ataagcgcat gatcgttcac ctaaacgttc atcgggcatt gcaaccacac
    20461 aggcatcgtg aattttagga tgctgcaata aaatatcttc cacttcacgg ctgctaatat
    20521 tttcgccgcc gcggacaata atatcttttt tgcgtccggt aatttttata tagccagctt
    20581 catccatacg gcagagatcg ccgctgtaat accagccttc ttcatccagc gcacgggcgg
    20641 ttaattcagg ttcatcaaaa tatcccataa acacattggg gccacgcgag gcttcttcac
    20701 cttcgcaacc tggcggtaag gtcttgcgtg cgtcatcgac cactttaatc tctacacctg
    20761 cggcagcgta accatcggtg tgcataaagc gcgacaaagg atcatcgaga ttcaccaccg
    20821 catgcggcga actttctgtg gaaccataaa cacttaataa tttaatgccg cgctgctggc
    20881 attcacgcgc cacttttttg ggaattgtgg taccgccgca aagaaagaag cgcagcgctg
    20941 aaaggtctgc gggttgtttc tctagtaaat tcaaaagatc atagacaaac ggcgttgcgc
    21001 cgagcataca agtgcaacgc tgctgctcaa gcagcgcgag acacgcatca ggagtgaaaa
    21061 tatctaacaa cacgctgcga gcgccaatta agaatggtgc cgttacgcca tgcagaaagc
    21121 ccgttgcgtg accaagtggc gcaggcatca taaagacatc ctgccaggtc agattcagcc
    21181 gcgcgcaata agcccgctca ctggcgagaa tattgttatg cgttagcatc acgccctttg
    21241 gcagaccctc ggttccggag gtaaacagca ccgcagctaa ttcatcgccg tgggtcgtta
    21301 tcgccgtggt cagtggggta ttgtcggcga taatctgact taatgagagg gaagaggtgg
    21361 cgggagccag tttgtccacg ccgacaattt gttgtagttg tggaagctga ttttgcagcg
    21421 gcaggattaa atctaccgga cgcgtttgtt taaacaacgt cggtgcaaag aacatttttg
    21481 cctgacactt attaagcacc cataccagtt ctgcttcccg ccaggaaggc aacagcggta
    21541 cggaaaccgc accgattttc aggcaggcaa gatagataac ggtaaattca caccagcctg
    21601 gcagttgaaa tgcgatgcga tcgcctgatt caataccctt cgctaacatc cagtttgcca
    21661 gacagctcgc ggcgtgatcg agtgcactat aggtgtacga tgctccatga ttatcgacca
    21721 cggcaatttt gtctggcatc gcacgagcgg tctgctgcca gtaatcggcc agcgaagcat
    21781 cgccccatag cccttgctga cgatacgccg cacgacgttg ttcgttaaac gttaatgtca
    21841 ctttcatttt ggactctcga aacagaacat caggcccgag atgcgggcct gtgggatgca
    21901 tgattagccg tagcgaaaat caataccgaa ggtgcctgcg ggatattgcc actgttcgag
    21961 aatagtgtta ccgcacagca cccgacaggt gccgcactcc agacaaccgg cggaatcaaa
    22021 atgaatgttg ccggcatcat cctgcttata aagtccggca gggcaggctt tcattaattt
    22081 acggaattca ttgatatcgg gatttgccgc caaaatgata tgcgggtgtc cctcatcaac
    22141 atggaattta ttgacgccta atttgatgtc gacgttaacc gtagcgttct ggctcatagc
    22201 gcggttgctc ccttaatgcc atctttcagc aagttgatca gcccaatttt cttcgcatgt
    22261 cccatgatca ttttgcgtac cggctggttt ggtttgccgt caatggtgaa catctcgttc
    22321 atgatgtcgg cgaccattcg tgggtattgg ctaaacaggc gcgggttttc catcagcgcc
    22381 gggatcttgc gaaaatgctg catatcacgc atgacgcagc tttgttccag ctcacgtttg
    22441 tattgcgcca gactgctggc ggagaaatcc gcgcgttctt tggcggcgat caccgttgtg
    22501 gcggcagcct gagccgatgc aatggctaaa tccatgccgc ggaccgtaaa acccaaattc
    22561 aggcagaagc ctgcggcgtc accaacgatc atcacgccct cgttaaccag ttgcggcacc
    22621 attgccagac cgccttccgg caccatatgc gcggaatatt caagcagttt gccgccgcta
    22681 atcagcgggc gaatggcggg gtgttgttta aaatcttcca gcatttgcgg cacgcttttt
    22741 tgcgcatggg cgatatcacc caatccacaa accagcccca aggatatgga atccttgttg
    22801 gtatagagaa atcccccgcc catcaggccg tcagaagggg aaccggcaaa cagccaggcg
    22861 gcaccttcct cgcccgtaat attaaagcga tcgttgatct gttctggtgt gaggccaata
    22921 acctctttaa caccaacagc gtaatgatgc ggatcggaag cgggaaccat tcccagcgag
    22981 cggccaagca tcgagttaac gccatcagcc agaatcacca cattcgcttc gagaatatca
    23041 tccccagcct gcacgccagt gaccttgttt ccttcacgaa ccagcgcgtc gacgcgaacg
    23101 cctgggataa actgcgcgcc cgcctgctcg gcttgttcca tcaaccacgg atccagacga
    23161 ttacgcaata cggtataaga tgcgtgttgc ggaacatctg gctgctcgcg gtgaaaatcg
    23221 agggtaacgg cgctttcttc ggttaagaag gagattttct cgcgtgtgac cttgcgttct
    23281 accggcgctg atactgcaaa gcctggaatg attgcttcaa gtgtgtgggc ataaagacgc
    23341 ccgccggtca tgtttttaca tccggcactg tcgccgcgtt ctatcaccag gacatccagc
    23401 ccggctcgtg ccatgaccag tgcggcaacg ctaccagcaa cgcccgcacc gaccacaatg
    23461 gcatcaaatt tgtcatccga catgcgttat gctcccttcc cgctgcgttt acagcgggag
    23521 atgtggttga aaggggaatt agcggctcaa ctggctaatc agggcaggga cgactttgta
    23581 gatatcgccc accagaccgt agtcggcata gttgaagatt ggcgcatttt tatctttatt
    23641 gatggcgaca atcacttttg cgccgttgcc gccaaccata tgctggatct gcccggagat
    23701 ccccagcgtc aggtagagat cggatttcag caacacgccg gagacaccga tataacgttc
    23761 acgctccatc cagttctcgc cttcggcaat tggacgtgaa cagccgactt cagcattcag
    23821 caccgccgcc agttcgtgga ccattttcag gtcatcctgc gccgccagtc cacgaccgac
    23881 gccaaccaca cgttttgctt tgctcaggtc cacgctgctt gcggctttcg cacggcgttc
    23941 gcgacagaga atttcatgac gcggagcaac ataaggtacc gtttctgtcg ggcactgatg
    24001 agaggtatca ctggtgcacg gttcctgaac accgggtgca agggtaatga tcgccagcgg
    24061 gctgttgatc ttttcctgag cgaacgctaa cccgccatac atccggtgtt cggcgcaaat
    24121 gtgaccatcg acaatatcca ccgccgtggc atcgttcacc agcgccgcat tcagttgcac
    24181 acttaaccgt gctgccagag ctttaccacg tttcgtcgcg gccaacagca acatagcggg
    24241 gtgtttatct ttcagcaggg cagcaatgct ttcggcgtaa ttttcagtgc gttgcagcgc
    24301 gtcgttttgc tcaagaacat aaatacattt tggaccataa ggcataactg cctgcgcctg
    24361 gtcggtattt tgtacaatgg catacacctg ttggccccat tgctgagcgc cgccaaacag
    24421 ttcagcataa cgttcaggat tatcgctaaa gacccagacg ctgtttaatt gactcatcgt
    24481 ttcttctccg taggcttcag ttcagggctt tcttcagatg ttccgccagc tcggcaatgg
    24541 cctccggcga atcgttatcg ataatgatgt gcttacgttc tgtttgcggc ggtacgcgaa
    24601 tgccgaccag ttcagcaagt ggcgcgctct ggctccagtc aatatcactt gcctgccact
    24661 gatttaccgg ttttttaccc gcgccgagaa tggctttcat cgaaggaatg cgtggcacgt
    24721 taatatcgga ggtgacgcag agcacggctg gaacagagag ttcaataact tcaacatcat
    24781 cttcaagcgt gcgttcaatc accagtgtat tgccctgacg ctgaatagca ctcactgcat
    24841 taatcaccgg aagttgcaga atttctccga ccagcaagcc aacctgctgg gcataaaggt
    24901 cgccggaacc ttcaccaaag atcagtaaat cgaagccgat cttttcaact gctgccgcca
    24961 gcgcttttgc ggtatcgaga ggcagtgcat gttcaagttg cgcatcctgc accaaataca
    25021 ggctgtgcgg cccgcgggat agcacgtctt tgcgcacttt cgagttctgc aacaatgagc
    25081 cgccaacggt cagcgcggct atctcatcgt catctgttgc gagctggctt gcagcttcaa
    25141 tggcattgag atcgaactgg ctgattttgg cgtcggcatt gtcgaaattc agggtgtatt
    25201 ctggagtgac aacaatgtcc tgttcttcag gcaccagctt aaagcaggtt attattttca
    25261 tgatttctcc tgtgagtcgc atacggaacc atctccgatg gaaggtaaaa atggcctgaa
    25321 aatagtgtgc gatagcggtg cggggttttc gacggagcaa gctgttttcc ttgccaacaa
    25381 tcttgcaaaa aaaatgcgat cgctaaaaca aaagtgcaat ttgcggcaat atagttattc
    25441 catgaccgta caatcttgca ttcagtgtaa ataatcttgt ttagctctcg ctaaacgcgg
    25501 ttgtataacc gcaatacacg ccgtatttta tccggcatat attgtttttg cttgctcatg
    25561 gaatagggag gttgtcatgt atcaacgctg ttttgataac gccagtgaaa cgctgtttgt
    25621 cgccggtaaa acgccacggc tttcacgttt tgcatttagc gatgatccaa aatgggagtc
    25681 cggacatcac gttcatgaca atgaaaccga gctgatttac gtcaagaaag gggtggcaag
    25741 gtttaccatc gattcttcgt tatatgtcgc acatgcggat gacattgtgg tgatagaacg
    25801 cggcaggctg catgcggtgg cctctgacgt taacgatccg gcaacgacgt gtacctgtgc
    25861 gctgtacggc tttcagtttc agggggctga ggaaaatcag ctactgcaac cgcattcttg
    25921 tccggtaatt gccgcggggc agggaaaaga agtcataaaa accttattta atgagctaag
    25981 tgtgattttg ccgcaaagta aaaatagcca aacatcttcg ttatgggatg catttgccta
    26041 tacgttagcc attctttact acgaaaattt taaaaat
//
LOCUS       DAPGOF010000060        26066 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.060, whole genome shotgun sequence.
ACCESSION   DAPGOF010000060 DAPGOF010000000
VERSION     DAPGOF010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26066
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.060"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(212..2785)
                     /gene="clpB"
                     /locus_tag="RHV13_002147"
     CDS             complement(212..2785)
                     /gene="clpB"
                     /locus_tag="RHV13_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDV1241678.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2915..3646)
                     /gene="pgeF"
                     /locus_tag="RHV13_002148"
     CDS             complement(2915..3646)
                     /gene="pgeF"
                     /locus_tag="RHV13_002148"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HDV1241679.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTDEPYDSKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAEASTAFIQHGDKYLADIYQLARQRLASVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3643..4623)
                     /gene="rluD"
                     /locus_tag="RHV13_002149"
     CDS             complement(3643..4623)
                     /gene="rluD"
                     /locus_tag="RHV13_002149"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HDV1241680.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKLCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4758..5495
                     /gene="bamD"
                     /locus_tag="RHV13_002150"
     CDS             4758..5495
                     /gene="bamD"
                     /locus_tag="RHV13_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HDV1241681.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5772..6113
                     /gene="raiA"
                     /locus_tag="RHV13_002151"
     CDS             5772..6113
                     /gene="raiA"
                     /locus_tag="RHV13_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDV1241682.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6216..6341
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6217..6264
                     /gene="pheL"
                     /locus_tag="RHV13_002152"
     CDS             6217..6264
                     /gene="pheL"
                     /locus_tag="RHV13_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HDV1241683.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            6363..7523
                     /gene="pheA"
                     /locus_tag="RHV13_002153"
     CDS             6363..7523
                     /gene="pheA"
                     /locus_tag="RHV13_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HDV1241684.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7566..8687)
                     /gene="tyrA"
                     /locus_tag="RHV13_002154"
     CDS             complement(7566..8687)
                     /gene="tyrA"
                     /locus_tag="RHV13_002154"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HDV1241685.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHITEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8698..9768)
                     /gene="aroF"
                     /locus_tag="RHV13_002155"
     CDS             complement(8698..9768)
                     /gene="aroF"
                     /locus_tag="RHV13_002155"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HDV1241686.1"
                     /translation="MQKDALNNVHITDEQVLMTPAQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            9978..10343
                     /locus_tag="RHV13_002156"
     CDS             9978..10343
                     /locus_tag="RHV13_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HDV1241687.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASAIRLN"
     gene            10493..11011
                     /locus_tag="RHV13_002157"
     CDS             10493..11011
                     /locus_tag="RHV13_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HDV1241688.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            11001..12227
                     /gene="dgcN"
                     /locus_tag="RHV13_002158"
     CDS             11001..12227
                     /gene="dgcN"
                     /locus_tag="RHV13_002158"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HDV1241689.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSAA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            12243..12725
                     /locus_tag="RHV13_002159"
     CDS             12243..12725
                     /locus_tag="RHV13_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDV1241690.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(12802..13149)
                     /gene="rplS"
                     /locus_tag="RHV13_002160"
     CDS             complement(12802..13149)
                     /gene="rplS"
                     /locus_tag="RHV13_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDV1241691.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(13191..13958)
                     /gene="trmD"
                     /locus_tag="RHV13_002161"
     CDS             complement(13191..13958)
                     /gene="trmD"
                     /locus_tag="RHV13_002161"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDV1241692.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQNEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(13989..14537)
                     /gene="rimM"
                     /locus_tag="RHV13_002162"
     CDS             complement(13989..14537)
                     /gene="rimM"
                     /locus_tag="RHV13_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDV1241693.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(14556..14804)
                     /gene="rpsP"
                     /locus_tag="RHV13_002163"
     CDS             complement(14556..14804)
                     /gene="rpsP"
                     /locus_tag="RHV13_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDV1241694.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(14941..16302)
                     /gene="ffh"
                     /locus_tag="RHV13_002164"
     CDS             complement(14941..16302)
                     /gene="ffh"
                     /locus_tag="RHV13_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDV1241695.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            16469..17260
                     /locus_tag="RHV13_002165"
     CDS             16469..17260
                     /locus_tag="RHV13_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HDV1241696.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            17281..18567
                     /locus_tag="RHV13_002166"
     CDS             17281..18567
                     /locus_tag="RHV13_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDV1241697.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSILLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(18622..19215)
                     /gene="grpE"
                     /locus_tag="RHV13_002167"
     CDS             complement(18622..19215)
                     /gene="grpE"
                     /locus_tag="RHV13_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDV1241698.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            19338..20216
                     /gene="nadK"
                     /locus_tag="RHV13_002168"
     CDS             19338..20216
                     /gene="nadK"
                     /locus_tag="RHV13_002168"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HDV1241699.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            20302..21963
                     /gene="recN"
                     /locus_tag="RHV13_002169"
     CDS             20302..21963
                     /gene="recN"
                     /locus_tag="RHV13_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDV1241700.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IVEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPETLPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQHYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            22112..22453
                     /gene="bamE"
                     /locus_tag="RHV13_002170"
     CDS             22112..22453
                     /gene="bamE"
                     /locus_tag="RHV13_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HDV1241701.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(22515..22805)
                     /locus_tag="RHV13_002171"
     CDS             complement(22515..22805)
                     /locus_tag="RHV13_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HDV1241702.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQKGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(22795..23232)
                     /gene="ratA"
                     /locus_tag="RHV13_002172"
     CDS             complement(22795..23232)
                     /gene="ratA"
                     /locus_tag="RHV13_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HDV1241703.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            23403..23885
                     /gene="smpB"
                     /locus_tag="RHV13_002173"
     CDS             23403..23885
                     /gene="smpB"
                     /locus_tag="RHV13_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDV1241704.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            24100..24462
                     /gene="ssrA"
                     /locus_tag="RHV13_002174"
     tmRNA           24100..24462
                     /gene="ssrA"
                     /locus_tag="RHV13_002174"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            24666..25907
                     /locus_tag="RHV13_002175"
     CDS             24666..25907
                     /locus_tag="RHV13_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDV1241705.1"
                     /translation="MARKTKPLTDTEIKAAKPKDADYQLYDGDGLTLLIKSSGSKLWQ
                     FRYYRPLTKQRTKQSFGAYPAVSLSDARKLRAESRVLLAKDIDPQEHQKEQVRNSQEA
                     KTNTFLLVAERWWNVKKASVTEDYADDIWRSLERDVFPAIGDISVTEIKAHTLVKAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LPQLMQTMRTANISQSTRCLFMWQLLTITRPAEAAEARWDEIDFEAKEWKIPAARMKM
                     NRDHTVPLSDGALAILAMMKSLSGGREFIFPSRIKPTQPMNSQTVNAALKRAGLGGVL
                     VSHGLRSIASTALNEQGFPPDVIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANL
                     IKAADSIPTVCNEVAGLRLVG"
BASE COUNT         6660 a         6832 c         6148 g         6426 t
ORIGIN      
        1 cggaattctc aggagaaccc cgctgacccg gcggcgtgtt tgccgttgtt ccgtgtcagt
       61 ggtggcgcat tatagggagt tattccggcc tgacaagcga aaaatataaa aactttatcg
      121 ttcgctcact tttcaggcaa aacatcttaa atatagtctt ttccgtctaa cttatagaca
      181 aaaacgagcc ccgaagggct cgttttatca tttactggac ggcgacaatc cggtcttcat
      241 taacttccag gcgaatcact ttacccggaa ccaattcacc agacagtatt tgctgtgcca
      301 gcgggttttc gatctgctgc tgaattgcac gtttcagagg acgtgcacca tagaccggat
      361 cgtaaccgtt ctcgctcagc agtttcagcg cctcgtcaga aatgtggatt tcataaccac
      421 gttcttccag acgtttatac agacgtttca actgaatctg cgcaatcgac gcaatgtgct
      481 gttcacccag cggatggaag accaccactt catcgatacg gttaatgaat tccggacgga
      541 agttatggct aaccacaccg agcaccagct ctttcatgtg cgcataatcc agttcaccga
      601 agcgttcctg aatcagatct gaaccgaggt tagaggtcat aatgacaacc gtattacgga
      661 agtcgaccgt tctcccctgc ccgtcagtca gacgcccatc atccagtacc tgcaacagaa
      721 tgttgaagac atccggatgc gctttttcca cttcatccag caggataacg gaatacggac
      781 gacgacgcac agcttcggtc aggtagccac cttcttcata accaacatat cccggaggcg
      841 caccaaccag acgagacacc gagtgtttct ccataaactc ggacatatcg atacggacca
      901 tcgcctcgtc gctatcaaac ataaagttcg ccagcgcctt acaaagctca gttttcccca
      961 caccagttgg gccgaggaac aggaatgaac caatcgggcg atttggatcc gccagccccg
     1021 cacggctacg acgaatagcg ttagataccg catcaaccgc ttcgttctga ccaataacgc
     1081 gatggtgcag ttcttgctcc atacgcagca gtttttcgcg ctcgctttcc atcatgcgag
     1141 aaaccggaat ccccgtccaa cgcgccagca cttcagcaat ttcggcgtcg gtcactttat
     1201 tacgcaacag acgcatagtt ttgccttcga gctgcgttgc ggcttccagt tgcttttcca
     1261 gttccgggat tttgccgtat tgcagttcag acatccgcgc caggtccccc acacggcgag
     1321 cctgttcaat agcgattttc gcctgttcca gttccgcttt aatggtctgc gtaccagaaa
     1381 gcgatgcctt ctctgctttc cactcttctt ctaactcgga gtactgacgt tctttgtcgc
     1441 tcagttcttc gttgagcata tccagacgtt ttttactggc ttcatcagac tctttcatta
     1501 acgcctgttg ttccagtttg agctggatga tacggcgatc gagtcggtcg agttcttctg
     1561 gttttgagtc aatctgcata cgaatgctgg atgctgcttc atcgatcagg tcgatggctt
     1621 tatccggcag ctgacggtca gcaatgtagc gatgagacaa cgtcgccgct gcaacaattg
     1681 ccgggtcagt aatttgcaca tggtggtgca attcgtaacg ttctttcagg ccacgcagaa
     1741 tcgcaatggt atcttcaaca gaaggctcgg caacaaacac tttctggaaa cgacgttcca
     1801 gcgcagcatc tttttcgatg tactggcgat attcgtcaag cgtcgtggca cctacgcagt
     1861 gcaattcacc acgcgccagc gccggtttca gcatgtttcc ggcgtccatt gcgccatcgg
     1921 ctttacccgc gccgaccatg gtatgtaatt cgtcgataaa caggatgacg ttgccttcct
     1981 gtttggcaag atcgttaagc acgcctttta aacgttcttc aaactcaccg cgatatttcg
     2041 ccccagccac cagcgcgccc atatccagcg ccagtacgcg gcggcctttc aacccttccg
     2101 gcacttcgcc gttgataata cgctgcgcca gaccttcaac gatggcagtt ttaccgacgc
     2161 cgggttcacc aatcagtacc gggttatttt tagtacgacg ttgcagcacc tgaatggtac
     2221 ggcgaatttc ttcatcacga ccaatcaccg gatcgagttt gccttgttcg gctcgttcgg
     2281 taagatcgat ggtatatttt ttcaaagcct gacgttggtc ttcagcacct tgatcgttca
     2341 cgctttcacc tccacgcatt tgttcaatcg cttgagtaat gttggcggtg gtcgcccctg
     2401 ctgctttcag gatgtcggcc agcgtgccgc gagactcaag tgccgccaga acgaacagtt
     2461 ctgacgagat aaagttatca ccacgttttt gcgccagctt gtcgcaaaga ttaagaacgc
     2521 gcaccagatc ctgtgatggc tggacatcac caccagtacc ttcaacctgc ggtaaacgat
     2581 ttaatgcctg attgatatct gtgcgcaact ggccagcatt tatgccagcg gatgttaata
     2641 aaggactaac cgaaccccct tcctgattca gcagggcgct cattaaatga agtggttcga
     2701 taaattggtt gtcgtgcccg agtgcaagtg attgggcatc ggcaagagca agctggaatt
     2761 tattagtaag acgatccaga cgcataactc ctcccataac ggatcaaagt tgctactgga
     2821 gattaaatga ggtcatccct caattattca aggttattga ccagattaat gtgaaaagaa
     2881 aatcacgcgt accggatcgt cttgattctt taggttatat cagccaaatg aaacttgcca
     2941 tacgaccggt ggtcttgtcg cgacgataag agaagaaagt ctcattttcc gtatatgtac
     3001 aacggtcgcc gccgaaaatt tgttcaacac caacgctcgc cagacgctgc cgggcaagct
     3061 gataaatgtc cgccagatac ttatcaccat gctgaatgaa agctgtactc gcttcggcgt
     3121 ctactgccat aaacgcctcg cgaacctccg cccccacttc aaacgcgcgt ggaccaattg
     3181 ccggccctaa ccaggcgaga atattttctg gattatcagc aaaacaggaa actgtctctt
     3241 ccagcacgcc tgcgcacagt ccacgccagc cagcatgagc ggcggcgact tccgttcccg
     3301 ctcgattgca aaacagcaca gggaggcagt cggcagtcat cactgcgcaa accgtgccgg
     3361 gcgtattgct ataagaggca tctgctcgtt ttgagtcata aggttcgtca gtaagcttaa
     3421 gcacatcttt gccgtgtacc tgctcaagcc agaccggttt agaaggcaaa ttgcccgcag
     3481 caaaaagtcg cttgcgattc tcctcaacgt gatccgggtt atcgccacaa tgggcaccga
     3541 ggttgagcga gtcatacggg ggcaagctca cgccgccgat acgagtagag ctacaggccg
     3601 caacaccttt tggcagcggc cactgcggga caatcagctt actcataacc agtccacttc
     3661 atctttatgc tcttcgaaat cggcgcgcat cacctcaatc agctccacca tatcttgtgg
     3721 aataggcgca tgccattcca tttcgatacc ggagatcggg tgataaagac gcagcatggt
     3781 tgcatgaagc gcctggcggt caaacttacg cagcgtggag ataaacgctt ccgaagcacc
     3841 ttttggcgga cgcggacggc caccataaac cggatcgccc accagcggat gagtgatatg
     3901 ggccatatgc acgcggatct ggtgcgtacg tccagtttcc agacgcaacc gcagacgcgt
     3961 gtgcacacgg aagtgttcca tgatgcgata gtgagtcacc gccggtttgc ccatcggatg
     4021 caccgccatg tgagtacgtt tggtcgggtg gcgactgatt ggctcatcca ccgtaccgcc
     4081 agcggtcata tgaccaatcg ccaccgcttc gtactcacga gtaatttcac gcctttgcaa
     4141 agattcgact aaacgcgtct gagccggaac ggtttttgcc acaaccatca ggccagtggt
     4201 gtctttatcc agacgatgga cgatgcccgc acgcggtaca tcggcaatag gcggataata
     4261 atgaagcaac gcattcagta ccgtgccatc cgggttaccc gcgccaggat gaaccaccag
     4321 gtcgcgcggt ttattaatga cgatgatgtc ttcatcttca tagacgatat ccagcgggat
     4381 atcctgcggt tcaaaacgcg cttcttcttc aatctctgcg ttgatggcaa cctgctcgcc
     4441 acccaatact ttttctttcg gcttatcaca aagtttgccg ttaaccagca cgcgctggtc
     4501 gaggatccat tcttttattc gcgaacgtga ataatccggg aacatttcgg ccaaagcctg
     4561 atctaagcgt tgtccgagtt ggttttcgga caccgttgca gtgagctgta ctcgttgtgc
     4621 catatatact gcttcttcgt ttatcgttgg ggttttacgg ctttgccgtt taatatagtg
     4681 tgctattgta gctggtctta accgggagca ggaacagaga atctcccgta ttacattttg
     4741 aggaaagtca aaacgtcatg acgcgcatga aatatctggt ggcagccgcc acactaagcc
     4801 tgtttttggc gggttgctcg gggtcaaagg aagaagtacc tgataatccg ccaaatgaaa
     4861 tttacgcgac tgcacaacaa aagctgcagg acggtaactg gagacaggca ataacgcaac
     4921 tggaagcgtt agataatcgc tatccgtttg gtccgtattc gcagcaggtg cagctggatc
     4981 tcatctatgc ctactataaa aacgccgatt tgccgttagc gcaggctgcc atcgatcgtt
     5041 ttattcgcct taacccgacc catccgaata tcgattatgt catgtacatg cgtggcctga
     5101 ccaatatggc gctggatgac agtgcgctgc aagggttctt tggcgttgac cgtagcgatc
     5161 gcgatcctca acatgcacga gctgcgttta gtgacttttc caaactggtg cgcggctatc
     5221 caaacagtca gtacaccacc gatgccacca aacgtctggt attcctgaaa gatcgtctgg
     5281 cgaaatatga atactccgtg gccgagtact atacagaacg tggcgcatgg gttgccgtcg
     5341 ttaaccgcgt agaaggcatg ttgcgcgact acccggatac ccaggctacg cgtgatgcgc
     5401 tgccgctgat ggaaaatgca taccgtcaga tgcagatgaa tgcgcaagct gaaaaagtag
     5461 cgaaaatcat cgccgcaaac agcagcaata cataacagaa acctgaaaca caaaacggca
     5521 gcccttgagc tgccgttttt ttattctgtt agttgtgaat gtgaaactga agcgatttag
     5581 tcactatcga tctcatcaaa tatggctcgc tttgagatat tcctcaagta aaaaaacacc
     5641 tcttcctgcg atttctcaca aaaaagattc gttgacaaaa agtgacaaaa ttatgagatt
     5701 tccatcacac attttgacat caggaacggt atgctgaatt caccaagacg ggaagacaag
     5761 aggtaaaatt tatgacaatg aacattacca gcaaacaaat ggaaattact ccggccatcc
     5821 gccaacatgt cgcagaccgt ctcgccaaac tggaaaaatg gcaaacacat ctgattaatc
     5881 cacatatcat tctgtccaaa gagccacaag ggtttgttgc tgacgccaca atcaatacac
     5941 ctaacggcgt tctggttgcc agtggtaaac atgaagatat gtacaccgca attaacgaat
     6001 tgatcaacaa gctggaacgg cagctcaata aactgcagca caaaggcgaa gcacgtcgtg
     6061 ccgcaacatc ggtgaaagac gccaacttcg tcgaagaagt tgaagaagag tagtccttta
     6121 tattgagtgt atcgccaacg cgccttcggg cgcgtttttt gttgacagcg tgaaaacagt
     6181 acgggtactg tactaaagtc acttaaggaa acaaacatga aacacatacc gtttttcttc
     6241 gcattctttt ttaccttccc ctgaatggga ggcgtttcgt cgtgtgaaac agaatgcgaa
     6301 gacgaacaat aaggcctccc aaatcggggg gcctttttta ttgataacaa aaaggcaaca
     6361 ctatgacatc ggaaaacccg ttactggcgc tgcgagagaa aatcagcgcg ctggatgaaa
     6421 aattattagc attactggca gagcggcgcg aactggccgt cgaggtggga aaagccaaac
     6481 tgctctcgca tcgcccggta cgtgatattg atcgtgaacg cgatttactg gaaagattaa
     6541 ttacgctcgg taaagcgcac catctggacg cccattacat tactcgcctg ttccagctca
     6601 tcattgaaga ttccgtatta actcagcagg ctttgctcca acaacatctc aataaaatta
     6661 atccgcactc agcacgcatc gcttttctcg gccccaaagg ttcttattcc catcttgcgg
     6721 cgcgccagta tgctgcccgt cactttgagc aattcattga aagtggctgc gccaaatttg
     6781 ccgatatttt taatcaggtg gaaaccggcc aggccgacta tgccgtcgta ccgattgaaa
     6841 ataccagctc cggtgccata aatgacgttt acgatctgct gcaacatacc agcttgtcga
     6901 ttgttggcga gatgacgtta actatcgacc attgtttgtt agtctccggc acgactgatt
     6961 tatccaccat caatacggtc tacagccatc cgcagccatt ccagcaatgc agcaaattcc
     7021 ttaatcgtta tccgcactgg aagattgaat ataccgaaag tacgtctgcg gcaatggaaa
     7081 aggttgcaca ggcaaaatca ccgcatgttg ctgcgttggg aagcgaagct ggcggcactt
     7141 tgtacggttt gcaggtactg gagcggattg aagcgaatca gcgacaaaac ttcacccgat
     7201 ttgtggtgtt ggcgcgtaaa gccattaacg tgtctgatca ggttccggcg aaaacgacgt
     7261 tgttaatggc gaccgggcaa caagccggtg cgctggttga agcgttgctg gtactgcgca
     7321 accacaatct gattatgacc cgtctggaat cacgcccgat tcacggtaat ccatgggaag
     7381 agatgtttta tctggatatt caggccaatc ttgaatcagc ggaaatgcaa aaagcattga
     7441 aagagttagg ggaaatcacc cgttcaatga aggtattggg ctgttaccca agtgagaacg
     7501 tagtgcctgt tgatccaacc tgatgaaaag gtgccggatg atgtgaatca tccggcactg
     7561 gattattact ggcgattgtc attcgcctga cgcaataaca cgcggctttc actctgaaaa
     7621 cgctgtgcgt aatcgccgaa ccagtgctcc accttgcgga aactgtcaat aaacgcctgt
     7681 ttatcgccct gctccagcaa ctcaatcgcc tcgccgaaac gcttatagta acgtttgatt
     7741 aacgccagat tacgctctga cgacataatg atgtcggcat aaagctgcgg atcctgagca
     7801 aacagtcgcc cgaccatcgc cagctcaagg cggtaaatcg gcgaagagag cgccagaagt
     7861 tgctcaagct gaacattttc ttctgccagg tgcagcccgt aagcaaaagt agcaaagtgg
     7921 cgcagtgcct gaataaacgc catattctga tcgtgctcga cggcgctaat acgatgcaac
     7981 cgagcgcccc agacctgaat ttgctccaga aaccattggt atgcttccgg cttacgtcca
     8041 tcacaccaga ccacaacttg ctttgccagg ctaccgctgt ccggaccgaa cattgggtgt
     8101 agacccaaca ccgggccatc gtgcgccgcc agcatggcct gtaatggtcc atttttcacc
     8161 gatgccagat caaccagaat acaatctttc ggtaaaggcg gtaatttgcc gataacttgc
     8221 tcagtaatgt ggattggcac actaacaatc accattccgg catcggcaac aatatcagcc
     8281 gctcgatccc agtcatgttg ctccagaatc cgcacctgat aacccgagag tgtcagcatc
     8341 ttctcgaaca gacgtcccat ctgaccgcca ccgccgacga taaccaccgg acgcagtgac
     8401 ggacaaagcg ttttaaatcc tttgtcgttt tcactggagt aagattctcg catcacccga
     8461 cgcaaaacat cctcaatcag atctggcggt acacccagag cttccgcctc tgcacgacgc
     8521 gaggccaaca tagatgcctc gcgctccgga acataaatag gcagtccaaa gcggcttttc
     8581 acctcaccca cttcagcaac cagttccaga cgcttcgcta ataaattcag cagcgcttta
     8641 tcgacttcat caatttgatc gcgtaatgcg gtcaattcag caaccataat aaacctctta
     8701 agccacgcga gccgtcagct gcccgttcag atcctgatga atttcacgca gcaaggcatc
     8761 ggtcatttcc cagctaatgc aggcatcggt tacggataca ccgtatttca tttcactgcg
     8821 cggttgctcg gaagactgat tgccctcgtg gatattactt tcgatcatca gaccaataat
     8881 tgagcgattg ccatctttga tttgagcaac cacggattct gccaccgcag gctgacggcg
     8941 ataatcttta ttggaattac cgtggctgca atctaccatc agagacgggc gcagtcccgc
     9001 ctgttccatc tctttttcac attgcgcaac atccgcaggg ctatagttcg gcgctttacc
     9061 accgcgcagg atcacatgtc cgtccggatt cccctgagtt tgtagcaacg caacctgccc
     9121 tgcctggtta atgccaacaa aacggtgcgg ctgggcggcg gcgcgcatag cgttaattgc
     9181 tgttgccaga ctgccgtcgg tgccgttttt aaaaccaacc ggcatggaaa gcccggaggc
     9241 catttcacgg tgagtttgcg attccgttgt acgagcacca attgctgacc agctaaacag
     9301 atcgcccagg tattgcgggc tattcggatc taacgcttcc gtcgccagtg gcagtcccat
     9361 attcaccagc tcaagcagca atttacgcgc gatctgcagc ccggcttcta catcaaaaga
     9421 gccatccata tggggatcgt taattaaccc tttccagccg acagtggtac ggggtttttc
     9481 aaaatagacg cgcattacca gatagaggct atcgctgacc tctgcggcaa gggctttaaa
     9541 tcgacgagca tattccagag cagtttccgg atcatgaatg gaacaaggac cacatactac
     9601 cagcagacga ggatcgcgcc cggcgataat atctgaaatg gttttacgcg agtcagcaat
     9661 ctgggcttct tgttgcaggc ttaatggaaa agcggccttc agttgtgccg gagtcattaa
     9721 aacctgttcg tcggtaatat gtacgttatt cagcgcgtct ttttgcatga tggcgatcct
     9781 gtttatgctc gtttgcgata gttgatcctc agcgaggatg acgtaacgat aacacataaa
     9841 gtaaagtttt caatccatat ttcgtacatt tttctttaca taagcaattt attcgcgctt
     9901 tcaacccttg attctgtata gataaattta caccaccttt gaaaacaatc cgctatgctt
     9961 tgaaaaagga gaaagaaatg atgaaaaagt ttatcgcccc cttgttggct ttactggtta
    10021 gcggatgcca gattgatcct tatactcacg cgccaacctt gaccagcacc gactggtatg
    10081 atgttggtat ggaagatgcg atatcgggca gcgccataaa agatgacgat gcatttagcg
    10141 attcacaggc ggatcgcggt ctatacctta aaggatatgc cgaaggacaa aagaaaactt
    10201 gccagaccga ttttacttat gcccgaggac tttccggtaa aagctttcct gccagctgca
    10261 acaatgttga aaatgccagc caactgcatg aagtatggca aaaaggggct gatgaaaatg
    10321 ccagcgcaat acgattaaat taattaaaac cccacaaata aattaaacat aagattttac
    10381 tcattatgtt ttatttctca caatgatgac gaccgcatga aataatgata ttttaatgag
    10441 cattctgaaa aaatatcgat gcatttcgag cgaagatggt ggggatccct gaatgcgttt
    10501 ttctcaccga ctgttcttac tgctgattct cctgctgacg ggagcaccaa tactcgctca
    10561 ggagccttct gacgttgcga aaaacgtgcg tatgatggtt tccggtattg tcagttacac
    10621 ccgctggcct gcgctatcag ggccaccaaa gttatgtatc ttttcatctt cacgttttag
    10681 taccgcccta caagagaatg ccgcgacatc tttgccttat ctgcctgtca tcattcacac
    10741 tcaacaagag gcgatgattt caggctgcaa tggtttttat tttggcaacg agtcaccgac
    10801 attccagatg gaattaaccg aacaatatcc gtcaaaggcg ttattattaa ttgccgaaca
    10861 aaataccgag tgcattattg gtagcgcctt ttgtctgatc atccacaata atgacgtcag
    10921 atttgccgta aacctggatg ccttatcgcg tagcggtgta aaggtcaacc cggatgtctt
    10981 aatgctcgca cggaagaaaa atgatggata acgataattc tcttaataag cgccccacgt
    11041 ttaaaagagc attacgcaac atcagtatga ccagcatatt tatcactatg atgctgatct
    11101 ggttgctgct ttccgtgacc tcggtgctga ccctgaaaca gtacgcgcaa aaaaacctgg
    11161 cactgacagc agcaacaatg acttacagtc tggaagcagc tgtcgttttt gccgatggcc
    11221 ctgcagcaac tgaaacactg gcagcgctgg gccagcaagg gcaattttca actgcagaag
    11281 tacgtgataa gcagcaaaat attctggcat cctggcatta cacccgtaag gatccaggcg
    11341 atactttcag caatttcata agccactggc tcttccctgc tcccatcatt cagccgattc
    11401 gtcacaatgg cgaaaccatt ggtgaagtac gcttaaccgc tcgcgacagt tcaatcagcc
    11461 attttatctg gttttcgctc gccgtactga ccggttgtat tctgctggca tcaggaatcg
    11521 caattaccct cacccgccat ttgcacaatg gcctggtgga agcactgaaa aatatcaccg
    11581 atgtcgtaca tgatgtgcgt tccaaccgca atttttcccg acgagtttcg gaagaacgta
    11641 tcgctgagtt tcaccgcttc gctctcgact tcaacagtct gctggatgaa atggaagagt
    11701 ggcagcttcg tttacaggcc aaaaatgcgc agcttctacg taccgcgcta catgacccat
    11761 taaccgggct ggctaaccgc gcagcgtttc gtagcggcat caacacgttg atgaacaatt
    11821 ccgctgcccg aaaaacgtcg gcgttactat ttcttgatgg cgataatttc aaatatatca
    11881 atgatacctg gggtcatgcg acgggcgatc gagtcttgat tgaaatcgca aaacggttag
    11941 ctgaatttgg cgggctacga cataaagcat accgcctggg cggcgatgaa ttcgcgatgg
    12001 tgctctatga tgtacagtcg gaatctgaag tgcagcagat atgctcagca ctgacacaaa
    12061 tctttaatct cccgtttgat cttcataatg gccatcagac caccatgaca ttaagcattg
    12121 gttacgcgat gaccattgag cacgcctctg cggaaaaatt acaagagctt gccgatcaca
    12181 atatgtatca ggccaaacac cagcgtgccg aaaagctggt gagataacaa ggatatatcg
    12241 atatgataaa gcacctggta gcacccctga ttttcacctc actaatactg actggctgcc
    12301 agtcccctca gggaaagttt actcctgagc aagtcgccgc tatgcaatct tatggattta
    12361 ctgaatcggc cggcgactgg tcgctgggct tatcagatgc cattctgttc gcaaaaaatg
    12421 actacaaatt gctcccggaa agccagcaac agatccaaac catggcagcc aaattggcct
    12481 cgacagggct aacacatgcc cgtatggatg gacacaccga taactatggt gaagacagtt
    12541 acaacgaagg cttatcattg aaacgggcga atgtcgtggc cgatgcatgg gctatgggtg
    12601 gacaaattcc acgcagcaat ctcaccacac agggtttagg aaaaaaatat cccatagcca
    12661 gtaacaagac cgcccagggc cgcgccgaga accgccgcgt cgcagtggtg attacaaccc
    12721 cttaaaacac gagcaaacag acaaaaaaag ggccagccaa ttggccagcc cttcttaaca
    12781 ggatgtcgct taagcgaaat cttagttaag acgctctttg atacgagcag ccttaccagt
    12841 acgctcacgc aggtagtaca gtttagcttt acgaacagca ccacgacgtt tgacagaaat
    12901 gctgtcaact accggagagt gagtctggaa gacacgctca acgccttcgc cgttggaaat
    12961 tttacgaaca gtgaatgcag agtgcagacc gcggttacga atagcgataa ccacgccctc
    13021 gaatgcctgc agacgttttt tggaaccttc aacaacccat actttcactt ccacggtatc
    13081 acccggacgg aaggaaggta cgtcctgctt catctgctct tgttcaagtt gcttaataat
    13141 gttgctcata atttaatctc ttatcctggg taaactgata tctcgggggc ttacgccatc
    13201 ccatcatgtt tatgttgctg ttgtgcgtgt tccgttttga actccgccag caaccttgct
    13261 tgctcttcag tcagagccag gttttccaga agttcaggtc ttctaagcca ggtacggccc
    13321 agcgactgtt tcaaacgcca gcgacgtatc tcggcatggt tgcccgacag taacactggc
    13381 ggaacttcca tcccttctaa cacctcaggc cgcgtatagt gcgggcaatc cagcaatcct
    13441 tcagcaaagg aatcttccgt tgccgaggct tcatgtccca gtactcccgg aataaaccgg
    13501 gaaacggagt caatcagcgt cattgctggt aactcaccac cactgagaac gtaatcgccg
    13561 attgaccatt cttcgtcaat ttcgttttgg atcacgcgct catctatacc ttcgtagcga
    13621 ccgcacacca gaatcaattt ttgattcgtt gccagttcgc tgacgcccgc ttgatcaagc
    13681 ttgcgtccct gtggtgacag ataaatcacc tttgcgcctt cacccgccgc ggcttttgct
    13741 gcatgaatgg cgtcccgcaa gggttgcacc atcattaaca tccccggtcc gccgccgtaa
    13801 ggacgatcgt ccacggtacg gtgccggtca tgcgtgaagt cgcgaggact ccagctctgg
    13861 atgctcagca ggccattttt aactgcccgg ccagttaccc cgtaatcggt aattgcgcgg
    13921 aacatttcag gaaacaggct aattatgcca atccacatag cgccgtcttt taccgtttat
    13981 ccggtggttt aaaaaccagg atcccaatct acttcgattg aacgagtagt gagatcgact
    14041 ttcttgataa cctgcccatc gaggaacggt acgagacgtt ccttgatacc aaacgcatct
    14101 ttcaggtttg ccttaatgac gagaacgtca ttagatccgg tttccatcat atcgacgact
    14161 ttaccgagat cgtagccttc agtggttact acctggcagc ccatcaggtc tttccagtag
    14221 tagtcaccct cttcaagctg aggcagctgc gatgaatcca cgacaatttc acaattcgtc
    14281 agcaggttcg ccgcatcacg atcgtcaacg cctttcagct tgatgatcat gtcctgattg
    14341 tggtgcttcc agctttccag ctggacttgc tgccactgac ccgccttctg gataaaccag
    14401 ggctgatagt caaaaatgct ttcggcgtct tcggtggaag aaaacactct gagccaccca
    14461 cgaataccgt aagacgaacc catttttccc aaaacgatgg gatcaacagg tgcttgcgcg
    14521 gtgagttgtt tgctcatcat gaccaccgtg acagattaag ctgctttgtt tacttctttg
    14581 atcagcgcag caacgcgatc agaaatagtt gcgccctggc caacccagtg agcgatgcga
    14641 tccagatcca ggcgagtgcc ttcttctttt tcgctagcga ttgggttgaa gaaaccaacg
    14701 cgctcgatga agcgaccgtt gcgtgcatta cggctgtcag cgacaacaac ctggtagaac
    14761 ggacgctttt tagcgccgtg acgtgctaaa cgaatagtta ccataacatc ctcttgtgtg
    14821 aataaaacaa ccggacccca tcgaggaacg gagtccggtg tcatattaaa agcccgaaaa
    14881 ttttactgat ttctggagaa aatgcaatca gcagttaata actctgtcgc ataaggtcat
    14941 ttagcgacca ggaaatcccg gcggcatcat ccccttcatg cttctcatca tcttcgccat
    15001 tccgcccttc ttcattttct tcatcatgcg ctgcatgtcg tcgaactgtt tcagaagacg
    15061 gttaacgtcc tgcacctgca taccgcaacc ggcagcaata cggcgtttac gcgaaccttt
    15121 gatgatttct ggcttagcgc gctctttcat cgtcatcgag ttgatgatgg cttccatacg
    15181 caccagcact ttatcgtcca tctgcgactt gacgttatcc gggatctgcc ccatgcccgg
    15241 cagcttgccc atcaggctag ccatgccgcc catatttttc atctggcgca gctgctcaag
    15301 aaagtcgttg agatcgaagc cgtcaccttt tttcagcttg ctggctaatt tctctgcctg
    15361 cgcgcggtca actttgcttt cgatatcttc gatcagcgac agtacgtcgc ccatgccgag
    15421 aatacgcgac gcgatgcggt ccggatggaa cggctccagc gcctcagttt tctcgccaac
    15481 accgaggaac ttgatcggtt tgccagtgat gtgacgaata gagagcgccg caccgccgcg
    15541 ggcatcgccg tccactttgg tcaacactac gccggtaagc ggtaacgctt cattgaatgc
    15601 ttttgccgta ttggccgcat cctgaccggt catggcgtca accacaaaca gggtttcaac
    15661 cgggttaatc gacgcatgga cttgtttgat ctcgtccatc atcgcttcgt caacgtgcag
    15721 acgaccagcg gtatccacca gcagcacgtc gtagaatttc agtttggctt ctttcagcgc
    15781 cgcgttaacg atatctaccg gcttctgacc aacatcagaa gggaagaaat caacgcccac
    15841 ctgctcagcc agcgtctcaa gctgtttgat tgccgccggg cgatagacgt cggcagaaac
    15901 caccaacact ttcttcttgt gcttctcgcg caggaactta ccgagtttac caacgctggt
    15961 tgttttaccg gcaccttgca ggcccgccat cagcacgacc gctggcggtt gcgcagccag
    16021 gttcagggtc tggttctctt cgcccatcgc cgcaaccagt tcgttacgga cgattttgac
    16081 gaactcctgc cccggcgtca ggctcttatt aacttcatga ccaaccgctt tctcttttac
    16141 gcgattgata aactcacgca ctaccggcag agcgacgtcc gcctccagca gcgccatgcg
    16201 cacttcgcgc agcgtatctt ttacgttgtc ttcagtgagg cgtccacggc cactgatatt
    16261 gcgcagcgtg cgcgacaaac gatcggttaa attatcaaac attgtctctc gcctggggtg
    16321 gaaacggttg gccgcaatcg cgacacatca tcagtatttt gccgcagtat aacatgaagg
    16381 cgtctttgtt gttatgcaac ggttggagca gcgttcacct gacgctatac tgcttctctt
    16441 tcttattgct caaactgtcg acattactat gcccgttttt gctctgctcg cgcttgtcgc
    16501 ctactccgtc agtcttgcgc tgattgttcc cggtctgctg caaaaaaacg gcggctggcg
    16561 gcgcatggct attatttctg cggtcattgc gctggtctgc cacgcaatcg ctctggaagc
    16621 ccgcatcctg cccgacggtg atagcggaca aaacctcagc ctgctgaacg ttggttcatt
    16681 ggtcagtttg atgatctgta cggtaatgac cattgtggct tctcgcaatc gtggctggtt
    16741 gctgctaccc attgtctatg cctttgcgct tatcaacctg gcgctggcga ccttcatgcc
    16801 caatgaatac atcacccatc tggaagcaac gcctgggatg ctggtgcaca ttggcttatc
    16861 gctcttttcc tatgccacgc taattatcgc cgccctgtac gcgctgcaac tggcgtggat
    16921 tgattaccaa ctgaagaaca agaagctggc gtttaaccag gaaatgccgc cattgatgag
    16981 tatcgagcgt aaaatgttcc acatcacgca gattggcgtg gtgctgctaa cgctcacgct
    17041 ttgcactggc ctgttctaca tgcacaactt atttagcatg gaaaatatcg acaaggctgt
    17101 gctctctatc gtggcgtggt ttgtctatat tgtgctgctg tggggacatt atcatgaagg
    17161 atggcgtgga cgccgcgtcg tctggtttaa cgttgcgggt gcagtcattc tgacactggc
    17221 ctacttcggc agccgaattg tccagcagtt aatcagctaa acccagaaaa ggagtttccc
    17281 ctggaacaca tttctactac tacgttgatc attattctga tcatcatggt ggtcatttca
    17341 gcctattttt ccgggtccga aaccggaatg atgaccctga accgctatcg tctgcgacat
    17401 atggcgaaac agggtaatcg ctcggccaaa cgcgtcgaaa aattgctgcg taagccagac
    17461 cgcctaataa gcctggtgtt aatcggcaat aacctggtca atattcttgc ctccgcgctc
    17521 ggcactattg ttgggatgcg tttgtacggc gatgcgggcg tggcaattgc gactggtgtg
    17581 ctgacctttg tggtgctggt ttttgccgag gtgttgccga aaactatcgc tgcgctatac
    17641 ccggaaaaag tcgcttaccc gagtagtatt ctgttagcac cgctgcaaat tttgatgatg
    17701 ccgctggtct ggttgctgaa tgctatcacc cgtatgctga tgcgcatgat gggtatcaaa
    17761 accgatatcg tggttagcgg ctctttgagc aaagaagagt tgcgcactat cgtgcacgaa
    17821 tcgcgctcac aaatttcccg tcgcaatcag gatatgctgc tgtcggtgct cgatctggaa
    17881 aaaatgaccg ttgatgacat catggtgccg cgcagtgaaa ttatcggtat tgatatcaac
    17941 gatgactgga aatctattct gcgccaactc tcccactcac ctcacgggcg catcgtgctc
    18001 taccgtgatt cgctggacga cgccatcagt atgctgcgag tacgtgaagc ctggcggttg
    18061 atgtctgaga aaaaagagtt caccaaagaa accatgctgc gcgccgcgga cgagatctat
    18121 tttgtgccgg aaggtacgcc gctcagcacg cagttggtga agtttcagcg caacaaaaag
    18181 aaagtcggcc tggtcgtcaa cgagtatgga gacattcagg ggctggtgac ggttgaagat
    18241 attctggaag agattgtcgg cgatttcact acgtcgatgt cgccaacact tgccgaagag
    18301 gtcacgccgc aaaacgacgg ttcggtgatt attgatggca ctgccaacgt gcgggaaatc
    18361 aacaaagcct ttaactggca tctaccggaa gatgatgccc gcacggttaa tggcgtcatt
    18421 cttgaggcac tggaagagat tcctgtcgca ggcacccgcg tgcgtattgg cgagtacgat
    18481 atcgatattc tcgacgtaca ggacaatatg attaagcagg taaaagtttt tcctgtgaaa
    18541 ccgctgcgcg agagtgtggc ggagtaacga aaacggcccg acattcgcat gcagggccgt
    18601 gaattattac gaaaacagaa attacgcttt tgctttcgct acagtaacca tcgccgcgcg
    18661 aatcgtacga ccgttcagcg tataaccttt ctgcataata cccagtacgt tacctggcgc
    18721 aacgtcatca gattccacca ttgcgatggc ctgatgcaca ttcgggtcca gcggcacgtt
    18781 agtttcggcg atcacttcaa caccaaactt acgcacaaca tccagcatcg acttcagcgt
    18841 cagctcaatg ccttcaacca tcgcagacat atccgggtta gctttatcag ccacttccag
    18901 cgcacgatcc aggctatcaa tcaccggcag caattcgttg atgaatttct ccagcgcgaa
    18961 tttgtgggct ttttcaatat ccagttcagt acgacgacgc aggttttcca tttcggcttt
    19021 tacacgcaaa atgccgtcac gttcacgggt ctgggcttca gccagctgag cttcgagatt
    19081 cgcaattttt tcatcgcgcg gatccacctg ctcagcagaa gcttctggct caactgcctc
    19141 aatctcttcg tgctgatcca tgataatttc ttccggggct tgcccctcag gcgttttctg
    19201 ttctttacta ctcatgaatt tctccgcgtt tttttcgcat tcatctcgct aacttcgctt
    19261 attatgggga tcagtttcag ggtttcaagg gaagcactca cattgtcatc aatcttcgca
    19321 acaaggacct cggaaaaatg aataatcatt tcaagtgtat tggcattgtg ggacacccac
    19381 ggcaccctac tgcactgaca acacatgaaa tgctctaccg ctggctgtgc acaaaaggtt
    19441 acgaggtcat cgttgagcaa caaatcgctc acgaactgca actgaagaat gtgaaaactg
    19501 gcacgctcgc ggagattggg caactagctg atctcgcggt agtcgttggt ggcgacggta
    19561 atatgctggg cgcggcgcgc acgctcgccc gttacgatat taaagttatt ggaatcaacc
    19621 gtggcaacct gggtttcctg actgaccttg accccgataa cgcccagcaa caattagccg
    19681 atgtgctgga aggtcactac atcagcgaga aacgtttttt gctggaagcg caagtctgtc
    19741 agcaagattg ccagaaacgc atcagcaccg cgataaatga agtggtactt catccaggca
    19801 aagtggcgca tatgatagag ttcgaagtgt atatcgacga gatctttgcg ttttctcagc
    19861 gatctgatgg gctaattatt tcgacgccaa caggctccac cgcctattcc ctctctgcgg
    19921 gcggtcctat tctgaccccc tctctggatg cgattaccct ggtgcccatg ttcccgcata
    19981 cgttgtcagc acgaccactg gtcataaaca gcagcagcac gatccgtctg cgtttttcgc
    20041 atcgccgtaa cgacctggaa atcagttgcg acagccagat agcactgccg attcaggaag
    20101 gtgaagatgt cctgattcgt cgctgtgatt accatctgaa tctgattcat ccgaaagatt
    20161 acagttattt caatacatta agcaccaagc tcggctggtc aaaaaaatta ttctaatttt
    20221 acgccagcct ctttactgta tataaaacca gtttatactg tacacaataa cagtaatggt
    20281 ttttcataca ggaaaacgac tatgttggca caactgacca tcagcaactt tgctatcgtt
    20341 cgtgagcttg agattgattt tcatagcggc atgaccgtaa taactggcga gaccggcgcg
    20401 ggtaaatcta ttgcaataga tgccctcggt ctttgtctcg gtggtcgcgc tgaagccgac
    20461 atggtgcgta ccggcgctgc tcgcgctgac ctgtgcgccc gtttttctct gaaagatacg
    20521 ccagcggctc tgcgctggct ggaagaaaac cagcttgaag acgggcatga atgtttgctt
    20581 cgtcgcgtga tcagcagcga tggtcgctcc cgtggtttca tcaacggtac agctgttcct
    20641 ctgtcacaac tgcgcgaact gggtcagttg ctgattcaga tccatggtca gcacgctcat
    20701 cagttactca ccaaacccga gcaccaaaag ttcctgcttg atggctatgc caatgaaacc
    20761 tctctactcc aggaaatgac cgcacgttat cagttgtggc atcaaagctg ccgtgacctc
    20821 gcgcatcatc aacagttaag tcaggaacgc gctgcccgtg cggaactgct gcaataccaa
    20881 ttaaaagaac ttaacgaatt taatccgcag cccggagagt ttgagcaaat cgacgaagag
    20941 tacaaacgtc tggcgaacag cggtcaattg ctgaccacca gccagaatgc attggcatta
    21001 atggccgacg gtgaagacgc aaacctgcaa agtcagcttt acacggctaa acaactggtg
    21061 agcgaattga ttggcatgga cagcaaactg tccggcgtac ttgatatgct ggaagaagct
    21121 accatccaga ttgttgaagc cagcgacgaa ctgcgccact actgcgatcg tctggatctc
    21181 gatcccaacc gactgtttga acttgaacag cgcatctcaa aacagatttc gctggcacgt
    21241 aaacatcacg tcagccctga gacattgcca cagtattacc agtcgctact ggaagaacag
    21301 cagcaactgg acgatcaggc cgactcacaa gaaacgctcg cgctggcggt aacgaaacat
    21361 catcagcagg cactggaaac ggcgcgcgca ttacaccagc aacgccagca ttatgcagaa
    21421 gaacttgcac agctgatcac cgacagtatg catgcgctct caatgccgca tgggcagttt
    21481 acgattgatg tcaaatttga cgagcatcac ctgggcgctg acggcgccga tcgtattgag
    21541 tttcgggtaa ccaccaaccc aggtcagcca atgcagccta ttgccaaagt cgcatccggt
    21601 ggtgaattgt cccgcatcgc attggcaatt caggtcatca cggcgcgtaa aatggaaacc
    21661 ccggcactga tttttgatga agtggatgta gggattagcg gtccaacagc ggcagttgtc
    21721 ggcaaactgc tgcgtcaact cggcgaatca actcaggtga tgtgtgttac ccacctgcca
    21781 caagtcgcgg gatgtggtca tcaacactat tttgtcagca aagaaaccga tggtgcgatg
    21841 acagaaacgc atatgcaatc cctgaataaa aaagcgcggt tacaagagct ggcgcgcctg
    21901 cttggtggca gtgaagtcac acgtaacaca ctggcgaatg cgaaagaact gcttgcggcg
    21961 taaacttttt tcctgcttca cggtcagagt aaacagcaaa acgccgtaag accggaaagc
    22021 aaaaggtttt aaagtgatga aaggtctatt atcatcggca tattacagat gagccacgta
    22081 ctgctcgggc ccgaaaagga atcaaatcac tatgcgctgt aaaacgctga ctgctgcagc
    22141 agcagtacta ttgatgttga ccgcaggctg ttccactctg gagcgagtgg tttaccgtcc
    22201 tgacatcaac caggggaact atctgaccgc taacgacgta tccaaaatac gtgttggcat
    22261 gacgcaacaa caagttgcgt acgcattggg tacaccgctg atgtccgatc catttggtac
    22321 gaatacctgg ttctatgtct tccgccagca accaggtcat gaaggtgtaa cgcagcaaac
    22381 gctgacgctg acctttaaca gtagcggtgt gttgaccaat attgataaca aacctgcgct
    22441 gagtggtaac taataataaa gttgctctca aagacgttaa aaaaggtgct caatgagcac
    22501 cttttttctg tctgttattt attcgctgat ttttctgctc gttgcctgcg aagctctttc
    22561 ggatcggcaa tgagagggcg ataaatctcc acccgatcgc catcatgcac actatcgctt
    22621 agttttgccg gacggctgta aatgccgact ttgtttttag ttaaatcgat atcggtacgc
    22681 aattccagca agccactggc gcgaatagct tcttcaaccg tcgcgccctt ctgcagcgtc
    22741 actcgctgca ggtactgctt ctcaggtagc gcataagcca cctcaacggc aattttacct
    22801 ggcactgtaa acctctttcg cacgaaccgt aaaagcctgg accatattag acgccagctc
    22861 tttaaacacg cgaccaaagg cgagctcaat caacttattg gtaaactcaa agtcgagatg
    22921 aaactcgata cgacacgcct cctggctcag cggcgtaaac ttccatccac caatcaattt
    22981 cttgaatggc ccatccacca gattcataag aatactttgg ttactggtca actggttgcg
    23041 ggtagtaaac gttttgctga tcccagcctt agagacatct accgccgcag tcatctgccc
    23101 aggagtggac tccagaatcc gacttccggt acaacccggc aaaaactgag gataagactg
    23161 aacgtcattc actaactgat acatttgctc cgcgctgtag ggtaccagtg cggtccggct
    23221 aatctgaggc ataacaattt ccatcaacaa aaatccaaca aataatatca tttatcctgc
    23281 tgtaaaaaaa acgctatccc ggcgctgggt aacatcgggt tcatgctaag atagagcctt
    23341 gtcccccgca ggattgatat ggggtgtttt cgatttcaga ttaccgatga ttcacgacgc
    23401 ttatgacgaa gaaaaaagca cataaacctg gttcagcgac catcgcgctt aacaagcgcg
    23461 cccgtcacga atactttatc gaagaagagt tcgaagcggg acttgccctg caaggctggg
    23521 aagttaaatc cctgcgcgca ggaaaagcca atatcagcga cagctacgtc cttctgcgtg
    23581 acggagaggc atttctgttt ggcgctaaca tcacgccaat ggccgtggcc tccacgcatg
    23641 tggtgtgcga ccctacccgt acccgcaagt tacttctcaa ccagcgcgaa ctggactcat
    23701 tgtacggtcg cgtcaatcga gaaggctata ccgtagtggc gctctccctg tactggaaaa
    23761 atgcctggtg caaagtgaaa atcggcgtcg ccaaaggtaa gaaacagcac gataaacgtt
    23821 cagatatcaa agagcgcgaa tggcaggtgg ataaagcgcg tatcatgaaa aacgcccacc
    23881 gttaaacctg cactccaatt attgaccagt tcctcaccgc gcctccctct ccggcggcgc
    23941 gaatgaacat cttattggct atcacatccg acacaaatgt tgccatccca ttgcttaatc
    24001 gaataaaaat caggctacat gggtgctaaa tctttaacga taacgccatt gaggctggtc
    24061 atggcgctca taaatctggt atacttacct ttacacattg gggctgattc tggattcgac
    24121 gggatttgcg aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa aagccgcaaa
    24181 aaatagtcgc aaacgacgaa aactacgctt tagcagctta ataacctgct tagagccctc
    24241 tctccctagc ctccgctctt aggacgggga tcaagagagg tcaaacccaa aagagatcgc
    24301 gtggaagccc tgcctggggt tgaagcgtta aaacttaatc aggctagttt gttagtggcg
    24361 tgtccgtccg cagctggcaa gcgaatgtaa agactgacta agcatgtagt accgaggatg
    24421 taggaatttc ggacgcgggt tcaactcccg ccagctccac caaaattctc catcggtgat
    24481 taccagagtc atccgatgaa gtcctaagag cccgcacggc gcaagccctg cgggcttttt
    24541 tgtatctgca atttgtcccg cgaagtccga agagaactaa ttaaatccga accttttagg
    24601 cccattgata ggcccaacga aaagctctat tgttttcgtt gggcctaaac gcatggagac
    24661 tccccatggc aagaaaaacc aagccgttaa ccgatacgga aatcaaagcc gccaaaccta
    24721 aagatgccga ttaccagctg tatgatggtg acgggcttac tctgttaatc aagtccagtg
    24781 gtagtaagct ctggcagttc cgttactatc gacctctgac aaagcagcga accaagcaga
    24841 gcttcggtgc ctaccctgcc gtctcacttt ctgatgcgcg taaactcaga gccgaatctc
    24901 gagttttatt ggcaaaagat attgatcctc aggaacatca gaaagaacag gtgaggaatt
    24961 ctcaagaggc caaaaccaac accttcttgt tagttgccga gcgttggtgg aatgtgaaga
    25021 aagccagcgt gacagaggac tatgccgacg atatctggcg ctcgcttgag agagatgttt
    25081 ttccagcaat cggtgatatc agtgtcactg agattaaggc tcatactctg gttaaagcag
    25141 ttcagccggt tcaggctaga ggtgcattag aaactgttcg ccgtctttgt cagcgtatta
    25201 acgaagtcat gatttatgcg cagaacacgg gcctgattga tgcggttccc agcgtaaata
    25261 tcggaaaagc gttcgagaag cctcagaaaa agaacatgcc gagcatccgg cctgatcaac
    25321 tgcctcaact gatgcagacg atgcgaacag caaacatcag ccaatcaaca cgatgcctgt
    25381 tcatgtggca gcttcttact attacccgtc ctgccgaagc cgctgaagct cgatgggatg
    25441 agatcgattt tgaagctaaa gaatggaaaa ttcctgcagc tcgaatgaag atgaaccggg
    25501 accatacggt tccattgtct gatggagctc tcgctattct ggcaatgatg aagtcactca
    25561 gtggtggccg agaatttatc tttcctagtc gaatcaaacc aacccagccg atgaatagcc
    25621 aaacagtgaa tgcagcactc aaacgtgctg gattgggagg cgttctcgtt tctcatggct
    25681 tgcgttctat cgccagtacc gcccttaatg aacaaggctt cccacctgat gttattgaag
    25741 cggcgctggc tcatgtggac aaaaatgaag tacgtcgcgc ttacaatcgc agcgattatc
    25801 ttgagcagag acgcccgatg atgcagtggt gggcaaacct tataaaagcg gcagatagta
    25861 ttcctaccgt ttgtaacgaa gtagcaggtc ttagattggt tgggtagtgg atttaaaatc
    25921 tcaagtgatt atccttagaa aagataatca cttgagataa ttgaaatttt tattggatta
    25981 aggcaaaacc atgctacatt aattaagata gtttaaaata gcatttatat agatatatgt
    26041 tacactttct aaaaaaaatc gcgaaa
//
LOCUS       DAPGOF010000061        25780 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.061, whole genome shotgun sequence.
ACCESSION   DAPGOF010000061 DAPGOF010000000
VERSION     DAPGOF010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25780
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.061"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(88..168)
                     /gene="shoB"
                     /locus_tag="RHV13_002176"
     CDS             complement(88..168)
                     /gene="shoB"
                     /locus_tag="RHV13_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HDV1241706.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(590..970)
                     /gene="acpS"
                     /locus_tag="RHV13_002177"
     CDS             complement(590..970)
                     /gene="acpS"
                     /locus_tag="RHV13_002177"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDV1241707.1"
                     /translation="MAILGLGMDIVEIARIEAVIARSGDRLARRVLSDNEWVIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVINMHVTLADERHYACATVIIES"
     gene            complement(970..1701)
                     /gene="pdxJ"
                     /locus_tag="RHV13_002178"
     CDS             complement(970..1701)
                     /gene="pdxJ"
                     /locus_tag="RHV13_002178"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HDV1241708.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(1713..2441)
                     /gene="recO"
                     /locus_tag="RHV13_002179"
     CDS             complement(1713..2441)
                     /gene="recO"
                     /locus_tag="RHV13_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDV1241709.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQAFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(2453..3358)
                     /gene="era"
                     /locus_tag="RHV13_002180"
     CDS             complement(2453..3358)
                     /gene="era"
                     /locus_tag="RHV13_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDV1241710.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(3355..4035)
                     /gene="rnc"
                     /locus_tag="RHV13_002181"
     CDS             complement(3355..4035)
                     /gene="rnc"
                     /locus_tag="RHV13_002181"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDV1241711.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(4308..5282)
                     /gene="lepB"
                     /locus_tag="RHV13_002182"
     CDS             complement(4308..5282)
                     /gene="lepB"
                     /locus_tag="RHV13_002182"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDV1241712.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(5298..7097)
                     /gene="lepA"
                     /locus_tag="RHV13_002183"
     CDS             complement(5298..7097)
                     /gene="lepA"
                     /locus_tag="RHV13_002183"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDV1241713.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(7295..7774)
                     /gene="rseC"
                     /locus_tag="RHV13_002184"
     CDS             complement(7295..7774)
                     /gene="rseC"
                     /locus_tag="RHV13_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HDV1241714.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(7771..8727)
                     /gene="rseB"
                     /locus_tag="RHV13_002185"
     CDS             complement(7771..8727)
                     /gene="rseB"
                     /locus_tag="RHV13_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HDV1241715.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(8727..9377)
                     /gene="rseA"
                     /locus_tag="RHV13_002186"
     CDS             complement(8727..9377)
                     /gene="rseA"
                     /locus_tag="RHV13_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HDV1241716.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(9410..9985)
                     /gene="rpoE"
                     /locus_tag="RHV13_002187"
     CDS             complement(9410..9985)
                     /gene="rpoE"
                     /locus_tag="RHV13_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HDV1241717.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(9982..10137)
                     /gene="rseD"
                     /locus_tag="RHV13_002188"
     CDS             complement(9982..10137)
                     /gene="rseD"
                     /locus_tag="RHV13_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HDV1241718.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            10393..12015
                     /gene="nadB"
                     /locus_tag="RHV13_002189"
     CDS             10393..12015
                     /gene="nadB"
                     /locus_tag="RHV13_002189"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HDV1241719.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGEESYHLTREGGHSHRRILHAADATGREVETTLVSKALNHPNIRVLERN
                     NAVDLIISDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYKKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(12000..12737)
                     /gene="trmN"
                     /locus_tag="RHV13_002190"
     CDS             complement(12000..12737)
                     /gene="trmN"
                     /locus_tag="RHV13_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HDV1241720.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTDDSVMIDAVELESEAATQAQENVAQSPWLERINVHT
                     ADIQQWVTQQTARFDLIISNPPYYEQGVECATPQREQARYTTSLDHQTLLTCAAECIT
                     EEGFFCVVLPEQIGNSFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            12869..14203
                     /gene="srmB"
                     /locus_tag="RHV13_002191"
     CDS             12869..14203
                     /gene="srmB"
                     /locus_tag="RHV13_002191"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HDV1241721.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKGRVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(14236..15117)
                     /locus_tag="RHV13_002192"
     CDS             complement(14236..15117)
                     /locus_tag="RHV13_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV1241722.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEHSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEDCFLPG"
     gene            15220..15807
                     /gene="eamB"
                     /locus_tag="RHV13_002193"
     CDS             15220..15807
                     /gene="eamB"
                     /locus_tag="RHV13_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HDV1241723.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATTHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQT
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWIVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLIYCAVRIFY"
     gene            complement(15863..16246)
                     /gene="grcA"
                     /locus_tag="RHV13_002194"
     CDS             complement(15863..16246)
                     /gene="grcA"
                     /locus_tag="RHV13_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HDV1241724.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            16551..17240
                     /gene="ung"
                     /locus_tag="RHV13_002195"
     CDS             16551..17240
                     /gene="ung"
                     /locus_tag="RHV13_002195"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDV1241725.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQHGETPI
                     DWMPVLPAESE"
     gene            complement(17288..18325)
                     /locus_tag="RHV13_002196"
     CDS             complement(17288..18325)
                     /locus_tag="RHV13_002196"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HDV1241726.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            18532..18951
                     /gene="trxC"
                     /locus_tag="RHV13_002197"
     CDS             18532..18951
                     /gene="trxC"
                     /locus_tag="RHV13_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HDV1241727.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            19020..19718
                     /gene="tapT"
                     /locus_tag="RHV13_002198"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             19020..19718
                     /gene="tapT"
                     /locus_tag="RHV13_002198"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HDV1241728.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            19750..22410
                     /gene="pat"
                     /locus_tag="RHV13_002199"
     CDS             19750..22410
                     /gene="pat"
                     /locus_tag="RHV13_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HDV1241729.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQQLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEDTCQKLRDALPGHV
                     AISNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQALIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAKAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMTWPQA
                     RIHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            22524..23879
                     /gene="pssA"
                     /locus_tag="RHV13_002200"
     CDS             22524..23879
                     /gene="pssA"
                     /locus_tag="RHV13_002200"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HDV1241730.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            23925..24248
                     /locus_tag="RHV13_002201"
     CDS             23925..24248
                     /locus_tag="RHV13_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HDV1241731.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(24245..25543)
                     /gene="kgtP"
                     /locus_tag="RHV13_002202"
     CDS             complement(24245..25543)
                     /gene="kgtP"
                     /locus_tag="RHV13_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HDV1241732.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
BASE COUNT         6238 a         6814 c         6434 g         6294 t
ORIGIN      
        1 tatagtatct gcccagacac ttatttatag ttattaaagg cgcgtccgat tggttcaccg
       61 gacgcacctt aagtacgttt ccttgtgtta taagaacaga aggatcagct gtaaaacagc
      121 aatgatgatt ttgatgaccc gtttaatcag gtatcggcaa tcagtcattc gtttttcctt
      181 aaacaaggaa tgcagccgtt cagattagcc cttacatctc cccaaaactg aacgtgcgag
      241 ttattggaat cgagtgctgc accccacacc agagctttga cgacaccact cgtttcaatg
      301 ggggaattca gtggcatggt gtaaagcaca gcaaaattgc caataacgaa accgatttta
      361 atgtacttat caaaaagcgg tcacgttaat cttttcatca atgtcgattt cttcgtaaag
      421 gctcgcgttt tttatgtcag agattgcaag ttgcttgtac aggtcaacga aattgatcca
      481 taatcatttt cgttggaatc gtgtgctgca caaaattaaa gttaaaaagt aaaacccccg
      541 ttccttacca gttcgggggt tttactttat aaagagaacg gtattatttt taactttcaa
      601 taattaccgt ggcacaagca tagtggcgct catcggcaag cgttacatgc atatttataa
      661 cgccgagctt ttccgccagt tttaatgcct caccccatag ccgtagccgt ggtttgccaa
      721 gctcatcgtt aaatacttca aactgattaa acgccagacc attgcggatc ccggtgccaa
      781 acgcttttgc cgccgcttct ttcacagcaa aacgcttcgc cagaaaacgc accggctggt
      841 ggtgcgtttt ccagataacc cattcgttat cgcttaatac gcggcgtgcc aggcgatcac
      901 cggatcgggc gatcaccgct tctatgcgag cgatctccac aatatccata cctaaaccta
      961 atattgccat tagccacgcg cttccagcat cagacgcttc atttctgcca ccgcatcttt
     1021 cagtccggtc atcactgcac gaccaataat ggcatgaccg atattcagtt cgtgcatctc
     1081 agggatggcg gcaatggctt ttacgttgtg ataggtcaga ccgtgtccgg cgttaacttt
     1141 caaaccgaga ctagcggcaa aggtcgcggc tttggcgata cgcgccagct cttgcgcctg
     1201 ttcggcgtca gttttggcat cagcatagca accagtgtgg atctcgataa acggtgcgcc
     1261 aacctctgcc gcagctttga tctgctcttc atcggcgtcg ataaacagag aaacctgaat
     1321 cccggcatcc gccagacgtt tgcaggcatc gcgcattttg tcacgctgcc ctgcaacgtc
     1381 caggccgcct tcggtcgtga cttcctgacg cttttccggt accaggcagc aaaaatgtgg
     1441 cttcgtctca acggcgatcg ccagcatctc ttcggtcact gccatctcca gattcatgcg
     1501 ggtatccagc gtctgacgca ggatgcgcac atcgcggtca gtaatgtggc ggcgatcttc
     1561 acgtaaatgc acggtaatgc cgtctgctcc cgcctgctca gcaataaacg ccgcctgcac
     1621 cggatccggg taagcagtac cgcgtgcgtt acgcagcgtt gcaatatggt caatattgac
     1681 gcctaacagt aattcagcca tgacaatcct catcattcat aatgtgtttt caccgttcgc
     1741 ttaggcataa actgccggaa cagttccctg ctctttaatg gtttaccgcc aagatacggc
     1801 ttaagcgcca tgcgggtaaa gcgtttcgcg gcgcgtagtg tgtctgcgtc aggaaattcc
     1861 cgtgcgttta acgcttttaa ctgccttccg gtgaacgttt tattgtcgat aacgacgctt
     1921 gcgataaacc ctttttcttc gcgataacga tacgtcatgg tgtcatctac cggctcgccg
     1981 ctacccgcgc aatgggtaaa attgacgcca tagcccagat gcccgagcag tgccagttca
     2041 aagcggcgca gcgcgggttc tggcgtacca gtggcacctg caagagactg aatgcagtgc
     2101 aagtaatcga aaaagagttc agagaagcgc gtctcgtatt ccagtacgcg ggagagaagt
     2161 tcgttgatgt acagaccgct gtaaagcgtg ataccgctta atggcagcgc cagcgagaca
     2221 gcttcagcac tgcgcagcgt tttgacttcg ccacgcccgc cgaaacgtag caagagaggg
     2281 gtgaaagcct gtaatgcacc tttcagggta gagcgtttag agcgtgcgcc tttggcaacc
     2341 agacgcacgc gacccgattc ctccgtgaag acgtccagca tcaggctggt ttcgctccac
     2401 gggcgactat gcaggacaaa tgcgcgctgc cagccttcca tcggagttac tcttaaagat
     2461 cgtcaacgta accgagactg cgcagtgcgc gttcgtcgtc ggcccaaccg gatttcactt
     2521 ttacccacag ttcaaggtgg acaggcgctt cgaacatttc ctgcatgtct ttacgcgctt
     2581 caatcccgat ggttttgatc ttggcccctt tgttgccaat aaccatcttc ttctgccctt
     2641 cacgctcaac gagaatcaaa ccattgatgt cataaccacc gcgttcgtta gagacgaaac
     2701 gttcgatctc cacggtcacg gagtacggca gttcagcgcc gaggaaacgc atcagttttt
     2761 cgcggatgat ttcagacgcc ataaaacgct gtgagcgatc ggtgatgtaa tcttccggga
     2821 agtgatgagt cgcttcaggc agatgcttac gcacgattgc cgcaatagtg tcaacattca
     2881 gcccggtttc ggcagagatc ggcacgatat cgaggaagtt catctggctt gccaggaact
     2941 gcaggtgcgg cagcagatcg gctttctcct gcacgttgtc cactttgttc accgcgagga
     3001 ttaccggcgc tttgccttcg cgcagtttgt tgagcaccat ttcgtcgtcc ggcgtccagc
     3061 gggtgccttc aacgacaaaa atcaccagct caacatcgcc aatagagctg ctcgccgctt
     3121 tgttcatcag gcggttaatg gcgcgttttt cttccatatg caggccaggg gtatcaacgt
     3181 agatcgcctg atacgcgcct tcagtatgga tccccacaat gcggtgacga gttgtctgcg
     3241 ccttgcggga ggtgatggag attttctgcc ccagcagttt gttcaacaat gtggatttgc
     3301 caacgttcgg acgtccgacg atggcaataa atccgcagta acttttatcg atgctcattc
     3361 cagctccagt tttttcaacg cctgttcggc ggcagcctgc tcagccttac gacggcttga
     3421 acctgtgcca accaccggtt cactcaggcc gctgacctgg cagtggattg taaattcctg
     3481 atcgtgcgct tcgccacgga cctgaactac cagataagtc ggcagcggca gatgacgacc
     3541 ctgcaaatat tcttgcaagc gcgttttcgg atctttttgt ttatcgcctg ggctaatttc
     3601 gtccagacga gtttgatacc agttgaggat taatttctcg acggtttgaa tatcactgtc
     3661 gaggaatacg ccaccaatta atgcttcgac ggtgtcggcg agaattgact cacgacgaaa
     3721 tccaccgctt ttaagttcac ctggtcctaa acgtaagcac tcgcctaact caaattcgcg
     3781 cgccagttcc gccagcgtat tgccacggac cagcgtggcg cgcatccggc tcatatcgcc
     3841 ttcatccaca cgagggaaac ggtgataaag cgcattggcg ataacgtagc tcagaataga
     3901 gtcgcctaaa aattctaaac gctcgttatg tttgctgctg gcactacgat gagttaatgc
     3961 ctgctgcaac agttcctgat gattaaaagt gtagcccagc ttccgttgaa gccgattaat
     4021 tacgatgggg ttcatgcgat accaataaat aaatgcgtca acaattcagc acacgaaaca
     4081 gacctgatat acatggctct gctaactgct tcgctgcagt ttcagtttag cggtatatgg
     4141 ggaccaacgc tgtttcgtgt gccgtggcaa cctggaggtg ccaaccttaa acttcggggg
     4201 aatattctat acacaacgac gggggatgtc gttagccacg ggagatttat ctcataaata
     4261 attcacgttg tcgccataac ggcgacaacg tgaacgaaga tgagctatta atggatgccg
     4321 ccaatgcgac ttaagcgcac accagtcggc cattcgcctt cttgcttatc gaagctcatc
     4381 cagatagccg ttgcccgacc gaccagattc gcttcaggca caaaacccca gtaacggctg
     4441 tccgcgctgt tgtcgcggtt gtcgcccatc atgaagtatt gtcccggcgg aacaatccag
     4501 gttgccagtt gttggcctgg ctgctggtaa tacatcccca cctgatcctg cgcaatcggc
     4561 actgtcagaa tgcggtgcgt cacatcaccc agtgtctctt tacgctcgga aagacgaatt
     4621 ccattttctt tggtttcgtt tttcggcact tcaaagaatc cgctggtcgc ttccccacca
     4681 ttacggcgtg agaaggtctg aacgaaatcg ctcggttcca cgtttgagta ggtgaccggc
     4741 agcgcgtttt cacacgcctg gccggaactg catcccggtt gaatcgtcag ctcttttgag
     4801 accggatcgt aagtgacttt atcgcccggt aaacccaccg cgcgcttgat gtaatcaagc
     4861 tttggatctt ccggatattt aaagaccacg atatcgccgc gtttcggatg accggtttcg
     4921 atcagcgttt tctggtagat aggatcttta atgccataag caaacttctc taccagaata
     4981 aaatcaccga ttaacagagt cggcatcatc gaacctgacg ggatctggaa cggttcataa
     5041 ataaacgaac gcacaatcaa tacgatagcc agtaccggaa aaacagaagc accggtttcc
     5101 agccagccag gcttcggcgc aacctttttc aacgttgctt tatccagtga gtcaccggca
     5161 gccgcctgcg ccgctgcctg acgttcccgc cgtttaggtg cgaaaaagaa tttatccacg
     5221 caccataaaa tgcccgtcac cagtgtggca atcaccagaa tcagggcaaa catattcgcc
     5281 atgccaactc ctaagggtta tttgttgtct ttgccgacgt gcagaatggc gaggaacgct
     5341 tcctgcggca gctcgacgtt accgatctgc ttcatgcgtt tcttaccttc tttctgcttc
     5401 tgcagcagct ttttcttacg gctgatatcg ccgccataac atttagccag tacgttttta
     5461 cgcagctgtt tcacggtgga tcgcgcaata atgtgcgtac caatcgctgc ctgaatggcg
     5521 atatcaaact gctggcgtgg gatcagatct ttcatcttct ccaccaactc gcgaccgcgg
     5581 ttttgcgaat tatcacggtg ggtgatcaac gccagcgcat caacacgttc accgttgatt
     5641 aatacgtcta cacgtaccat gtcggacgcc tggaagcgtt tgaagttgta atccagagac
     5701 gcataaccac gcgaggtaga tttcaggcga tcgaagaagt cgagcaccac ttccgccatc
     5761 gggatctcgt acgtcagcgc cacctgatta ccgtggtaaa ccatattggt ctgcacgccg
     5821 cgtttttcta cgcacagcgt aataacgttg ccgagatatg cctgcggcag cagcatgtga
     5881 cactctgcaa tcggctcgcg cagttcgtaa atgttattta ccgcaggcag cttggatggg
     5941 ctgtcgacgt agataacttc ccttgacgtg gtttcaactt catacactac ggtcggcgca
     6001 gtggtgatca gatccagatc gtattcacgt tccagacgct cctggatgat ctccatgtgc
     6061 agcaggccaa ggaagccgca gcggaaacca aagcccagcg cgctggagct ttccggctca
     6121 tagaacagtg aggcatcgtt caggctgagt ttacccagcg cgtcacggaa ggcttcatag
     6181 tcgtcggaac ttaccgggaa cagaccggcg tatacctgcg gtttgacttt cttaaagcca
     6241 ggcagcgcct tttctgccgg attacgcgcc agcgttaagg tatcgccgac tggagcgccg
     6301 tggatatctt taatcgcaca tacgagccag cctacttcgc cacatttcag ttcagtgcgg
     6361 tcaacctgtt tcggcgtgaa gatgcccaga cggtcggcgt tataggtctg cccggtactc
     6421 atgactttca ctttgtcgcc cttacgcagg gtgccgtttt taatacggat aagtgaaaca
     6481 acgcccaggt agttgtcgaa ccatgagtcg ataattagtg cctgcaacgg gccttccgga
     6541 tcgccttccg gcggcggaat gtcgcgcacc agacgttcga gaacgtcctg cacgccaacg
     6601 ccggttttcg ctgaacagcg caccgcgtca gtggcgtcga tgccgacgat atcttcaatt
     6661 tcttccgcca cgcgttcagg atcggctgcc ggcaggtcaa tcttgttcag taccggcacc
     6721 acttcgagat ccatttccat ggcggtgtag cagtttgcca gggtttgcgc ttccacgccc
     6781 tgcccggcgt caaccaccag caatgcacct tcacaggcag ccagcgaacg ggaaacttca
     6841 taggagaagt ctacgtggcc cggggtgtcg ataaagttaa gctgataggt ttcgccgtca
     6901 gacgctttgt agtccagcgt cacgctttgc gctttgatgg taatgccacg ctcacgctca
     6961 agatccatgg aatcgagaac ctgcgcctcc atttcacggt cagacaggcc accgcagatc
     7021 tggataatac ggtcagacag cgtcgattta ccgtggtcaa tgtgagctat gatcgaaaag
     7081 ttacgtatat tcttcataaa gtatgattat tgtgccttaa tgcccggtta accaggcttt
     7141 tagaagtcgc tgttctgagc ttaacgtctg tattaataga aacgccgcat tctacactac
     7201 aacattgagg cgaggaaatg ttcataccgt atggattgtg gtatctggaa acgtcctcgc
     7261 atttgttatg caaaatgcaa caaagccagt gaaatcactg gctcgcgtct tccgaagatg
     7321 tttcaaatcg cacaaggcca ggcggcaatg ccacgcttaa gatgatcggt tgccattctg
     7381 cccgggcagc aaacttacgc gagtagccac gtgcaatcag gaacccgcca ataccgccga
     7441 ggaccgcacc acataatgct gcgacgtcag aagcaaagag tagctgaaat agcgaagcga
     7501 tgaggaataa tcccaccagc ggcgacatat aaaccagtaa tgcggagcta agcaggctgc
     7561 cttcggcgat ccccaattcc actttttgcc ccggcactaa cggctcatca cagggtacga
     7621 caatggtatg cgtggtttgc gggccaagtt tattcaacac gcggctaccg caaccggcgc
     7681 gtgaagcgca gctgctgcat gaggctttaa catcacaact gaccagcgcc tgcccgtttt
     7741 gccaggagac gacggtagcc cactctttga tcattgcgct gccccgaact taatattctc
     7801 ggcaatgcgt ttcgccgttt gcggcggcag ttcaccgaca atggtgattt cggcgttatc
     7861 acgtacgctt gtactgacgg ttctgcgtcc ggtgcgcaac atctgatcgg tgctcgatgg
     7921 cgtagcgcgg ttaacgttta ccgagaagct gaataatccg tcggaataga gacgtgattc
     7981 gataggcatg ttgtccatcg tcggtagcgg acgtcgacta ctggaaactt cgctaaaacc
     8041 ctgtggcaac caggttggcg tccagctgaa tttagctttt tcacctacag gaacagaaag
     8101 caacggcggc aaatttgcct tcgccagcgt ctgcatactg ctgctgatat cctgattgac
     8161 gttaaaagca atcacgcgaa attgttccag cgtttcacca tcgcgatcaa gaagatcaac
     8221 ccgcatcggt aatttcgatt cggtgtccat ccacacgatg tagctgtagc gtgtaccatc
     8281 tcgggcaacc acgcgaatga cttcgcaaag acgatcagca atacgcgtgc gcccgacgga
     8341 gataaagtcg tagtaaggag aaaggcgttt gaaatcggta tagatgagcg atggcagaga
     8401 atcaacaatg taatcgccat taagcgtgaa cggttcaagt cccggttcaa aatagctgat
     8461 ttcattgccg cgctgtacca cttcccggcg cgggccatcc atttgcaaca attgtgcaag
     8521 aggacggtta tcgaggcgtg catgtcgata acgcagagac tcaacaccct gtttattgat
     8581 gctgatgaat gacagctcgt aattcagtga ctgactggcc aggttcatct gctgtaataa
     8641 cgccccggac gcgggagtgg ccgaggcgtt agcagagaat aacaggctac ctgtcactaa
     8701 tgacatggca aaccaaagtt gcttcattac tgcgattgcg ttcctaaagt ttgaattcct
     8761 ggcacctgta cagcggcttg ctgggtttgt gcctgctcaa actgaagctg ttcagagtgg
     8821 agtcggcgtt gcagttcgta atcctgcaac attgcattaa tgcgacgacg ctgctcctgt
     8881 acctgctgct gttgaccatt gtttgcggtc gcttcagaag gtactcccag gctaaccggg
     8941 ctggctttac ccatcatcgg cagtgtatta aataccggcg tttcgggctg ctgggacgtt
     9001 tcagattgtc cattatagtg ctggacgcca acgataactg caagcgatac gcatgcggct
     9061 acgcccattt gggtaagctg tgccgcccac ggacgtactt tctgccagaa tggcattttc
     9121 tgccattgat gcggcgcagg ctgggcttcc gggatcaatg tcgccggttg acgtactggc
     9181 tcttcttcaa tggcggccat cacgcgtgaa gagatatcga aatggagcac ctcgggagta
     9241 tcaccccgca ttgagtcacg gattaagtga tagctttccc aggttttctg catttctggg
     9301 ttatgagcca gttcgttaag cagctcacta tccagcgttt cgccatccat taaagcggaa
     9361 agttgttctt tctgcatgcc taataccctt atccagtatc ccgctatcgt caacgcctga
     9421 taagcggttg aactttgtta tcaatagctt ccctcgctcg gaagatacgt gaacgcaccg
     9481 tacctaccgg acaatccatg atagcggcta tctcttcata gctcaggcca tccagctccc
     9541 gcaaggttat tgccatgcgt aaatcttccg ggagggactc aatagttcgg aaaactatct
     9601 gtctcagttc ttctgacaac attaagttct cagggttcga aatttctttc aacgcgccgc
     9661 cactttcgaa gttttcagct tcaatggcat ccacatcact ggaaggtgga cgacgcccct
     9721 gagcaaccag gtaatttttc gctgtattta cagcaatccg atacagccat gtataaaaag
     9781 cgctatctcc ccggaacgaa tccagcgcac gataggcttt aataaaagct tcttgtacca
     9841 catcgggaac atcacccgac ggcacatagc gggaaaccag actcgccact ttatgctgat
     9901 agcgcactac cagtaagtta aaggctttct gatctccctt ctggacccgt tcaaccagga
     9961 cctggtccgt taactgctcg ctcatccgag gtaaagtctc cccaaaccaa atttccacgc
    10021 gctatcgaaa cgccactcca ttagctgcaa tttgagcaag caaagggtta gagtgtctcg
    10081 tttttgtaaa gttccgtaac gcatctgttt ttgtttgtca tgctgtagac ggatcattat
    10141 ctatcattat aagtctacag aatctgaaca tcgcattatc tgtgtagaaa tgcccattta
    10201 actgcctgaa gagtaaccca acggcctttt tatttcacca cctaatcctc caccagccag
    10261 taacttctct ttttctcgcc gccctgcgtc agcgtgttta gccactgtaa caaatattaa
    10321 aatagcaggt gtttattcgc acaacatgat gctatgctga ccaaaccgtg tttagtaaat
    10381 taaacaaaga aaatgaatac tctccctgaa cattcatgtg acgtgttgat tatcggtagc
    10441 ggcgcagccg ggctttcact ggcgctacgc ctggctgacc agcatcaggt catcgttcta
    10501 agtaaaggcc cggtaacgga aggttcaaca ttttatgccc agggcggtat tgccgccgtg
    10561 tttgatgaaa ctgacagcat tgactcgcat gtggaagaca cattgattgc cggggctggt
    10621 atttgcgatc gccatgcagt tgaatttgtc gccagcaatg cacgatcctg tgtgcaatgg
    10681 ctaatcgacc agggggtgtt atttgatacc cacattcagc cgaatggcga agaaagctac
    10741 catctgaccc gtgaaggtgg acatagtcac cgtcgtattc ttcatgccgc cgacgcaact
    10801 ggtagagaag tagaaaccac gctggtgagc aaggcgctga accatccgaa tattcgcgtg
    10861 ctggagcgca acaacgcggt tgatctgatt atttctgaca aaattggcct gccgggcacg
    10921 cgacgggttg ttggcgcgtg ggtatggaac cgaaataaag aaacggtgga aacctgccac
    10981 gcaaaagcgg tggtactggc aaccggcggt gcgtcgaagg tttatcagta caccaccaat
    11041 ccggatattt cttctggcga tggcattgct atggcgtggc gcgcaggctg ccgggttgcc
    11101 aatctcgaat ttaatcagtt ccaccctacc gcgctgtatc acccacaggc acgcaatttc
    11161 ctgttaacag aagcactgcg cggcgaaggc gcctatctca agcgcccgga tggcacgcgt
    11221 tttatgcccg attttgatga gcgcggcgaa ctggccccgc gcgatattgt cgcccgcgcc
    11281 attgaccatg aaatgaaacg cctcggcgcg gattgtatgt tccttgatat cagccataag
    11341 cccgccgatt ttattcgcca gcatttcccg atgatttata aaaaattgct cgggctgggg
    11401 attgatctca cacaagaacc ggtaccgatt gtgcctgctg cacattatac ctgcggtggt
    11461 gtaatggttg atgatcatgg gcgtacggac gtcgagggct tgtatgccat tggtgaggtg
    11521 agttataccg gcttacacgg cgctaaccgc atggcctcga attcattgct ggagtgtctg
    11581 gtctatggct ggtcggcggc agaagatatc accagacgta tgccttatgc ccacggcgtc
    11641 agtacgttac cgccgtggga tgaaagccgc gttgagaacc ctgacgaacg ggtagtaatt
    11701 cagcataact ggcacgagct acggctgttt atgtgggatt acgttggcat tgtgcgcaca
    11761 acgaagcgcc tggaacgcgc cctgcggcgg ataaccatgc tccaacaaga aatagacgaa
    11821 tattacgccc atttccgcgt ctcaaataat ttgctggagc tgcgtaatct ggtacaggtt
    11881 gccgagttga ttgttcgctg tgcgatgatg cgcaaggaga gtcgtggcct gcattttacg
    11941 ctggattacc cggaactgct cacgcattcc ggcccatcga tcctttctcc cggcaatcat
    12001 tacataaaca gataaaaagc ctgggttagc gccgtatacg cttcggaata gttctggtcg
    12061 ggcccacgaa tgactaagcg atcgctaaag cattctcccg cctgcgggga gaatgccagc
    12121 agcacccgat gcggcagtcg cgcttcgttt tccgccacat ccgtccgcaa acgtaaatgc
    12181 cagcccatac ttaatgccag ttcagtaaaa ctattaccaa tctgctctgg cagcaccacg
    12241 cagaaaaatc cctcttcggt aatgcactcc gccgcgcaag taagtaaagt ctggtgatca
    12301 agtgaggtgg tgtaacgcgc ctgttcccgc tgcggcgtcg cgcactccac tccttgctca
    12361 taatacggcg ggttgctgat gattaaatca aagcgcgcgg tctgctgtgt tacccactgc
    12421 tgaatatccg ccgtatggac gttaattctc tctaaccacg gggactgggc gacattttcc
    12481 tgcgcctgag tggcagcttc actttccagc tcaacggcat caatcatcac gctgtcatcg
    12541 gttcgctgcg ccagcattaa tgccagcaac ccgctccccg caccgatatc aaggcagcgc
    12601 tttacccccg ccaccggtgc ccatgcgccc agtaaaatgc catccgttcc cactttcatc
    12661 gcacagcgat catgagcaac aaaaaactgt ttaaaagtaa atccattacg acgaagcacg
    12721 gatgtagact gtgacatgaa aataaaacct tgcaggaaaa acggcgatag caccgggtga
    12781 gaacaatacc cgagaagcga tatccataca aacagatgaa gattgcagcc gtaacgtcta
    12841 taatcagcgc cccacacaga ggtagaacat gactgtaacg actttttccg aacttgaact
    12901 cgacgaaagc ctgctggaag ccctccagga taaaggtttc actcgcccga ccgccattca
    12961 ggctgccgcc attccgcctg cgctcgatgg ccgtgatgta ctcggttctg cgccgacagg
    13021 caccggtaaa acggcggcgt atctgctgcc agcgttgcag cacctgctcg atttcccgcg
    13081 taagaaatcc ggtccgccgc gtattttgat cctcacccca actcgcgagc tggcgatgca
    13141 ggtggccgat catgcccgcg aactggcgaa acatacgcat ctggatatcg ccaccatcac
    13201 cggcggcgta gcctatatga accacgcgga agtgttcagc gaaaatcagg acatcgtggt
    13261 cgccacgacc ggacgtctgc tgcaatacat aaaagaagag aacttcgatt gccgcgcggt
    13321 tgaaacgctg atcctcgacg aagcagaccg tatgctggat atgggcttcg ctcaggatat
    13381 cgaacatatt gctggcgaaa cgcgctggcg taaacagacc ctgctctttt cggcaactct
    13441 ggaaggcgat gcgattcagg actttgccga gcgtctgctg gaagatccgg tggaagtttc
    13501 tgccaatccg tccacccgtg agcgtaaaaa aattcatcag tggtattacc gcgccgatga
    13561 tcttgagcat aaaaccgcgt tgctggtgca tctgttaaaa cagccggaag cgacccgctc
    13621 aattgtgttt gtgcgtaagc gtgaacgtgt gcatgagctg gcaaactggc tgcgcgaagc
    13681 gggcatcaac aactgctatc tcgaaggtga gatggtacaa ggcaagcgta acgaagcgat
    13741 caagcgtttg accgaaggtc gcgtaaacgt actggttgca accgatgttg ctgcgcgcgg
    13801 tatcgacatt cctgacgtca gccacgtctt taacttcgat atgccgcgca gtggcgatac
    13861 ttatttgcac cgtatcggac gtaccgcgcg cgccggtcgt aaaggcaccg caatttcgct
    13921 ggtagaagcc catgaccatc tgctgctggg caaagttggt cgctatattg aagagccaat
    13981 taaaggtcgc gtaattgatg agttacgccc gaaaacgcgt gcaccaagcg agaagcagac
    14041 cggcaagcca tcgaagaaag tactggcaaa acgcgccgag aagaaaaaag ctaaagagaa
    14101 agagaagccg cgggtgaaaa aacgccatcg cgataccaaa aatattggta agcgccgtaa
    14161 accaagcgga acgggcgtgc caccgcaaac gacagaagag taatctcaat gccgggttta
    14221 agcccggcat tatttttatc ccggcaaaaa acaatcttca acacactgaa taaaagtgtg
    14281 catcgccggg ctaaccgctt ttccggcatg gtgagcgcac attgccgtaa tggtctgcga
    14341 gtgttcgcca aaaggcaatt caattaattc tccgctctca agctcttttt ccaccgcaaa
    14401 gcggggcaag taactgacac cgatattcgc cgcgacacaa cgtttgatac tttcaatgct
    14461 taacagctca atggtgtttt ccaccgtgat ccgccgctgg cgcagcgtgc tctcaaatat
    14521 ctgccggaat acacattgcg gttcgttgat aataaagcta caggcgttat gtcttcccgg
    14581 ctcagtaaaa tcgacatctg caatttgcgg tgaagctacc agcaccagcg attgctcacc
    14641 caattctcgt cggttcaggg catcatcatt ccctacgcga taaaaaaccc cgacatcggc
    14701 ctcatcattc aatagcgcat cacggatcac gtaacagttc agagactgca acgataaacg
    14761 cacttttggg gcgcgttgcc gaaaacgctg caacacctgc ggcattcggt aagagagcaa
    14821 cgtttcgccc gaaacaacgc gtagttcccc gtccggatcc gactcctttt tggcagcctc
    14881 acgaagggta tccatcactc tggtgagttc ataaatgtgc ggcagcagct ttttaccttc
    14941 gcgagtgagg cacatccgtc ggccaatttt ctcaaataac tgtaccgaga actcctgctc
    15001 aagctgctga atatgaaaag tcaccgtcga ttgtgtacag cacaattttt gcgaagctcg
    15061 caaaaaggaa ccctcttcca ccacggtttt aagcgtaata aaacggcgca gatccataac
    15121 cccaaaccta tcgaaaatat cgaatttaga atgtaaaaac attcattttt ttaaatgttc
    15181 cgtgtcgggt actgtctacc aaaacagagg agataacaag tgacaccgac ccttttaagt
    15241 gctttttgga cttacaccct gattaccgcc atgacgccag gaccgaacaa tattctcgcc
    15301 cttagctcag ccacaacgca cggatttcgt cagagtaccc gcgtgctggc agggatgagt
    15361 ctgggatttt taatcgtcat gttgctgtgt gcgggcattt cgttttcact ggctgttatc
    15421 gacccggcag ctgtgcatct gttgagttgg gcgggggcgg catatattgt ctggctggcg
    15481 tggaaaatcg ctaccagtcc aacaaaggaa gacgggcttc agacaaaacc aatcagtttt
    15541 tgggccagct ttgctttgca gtttgtgaac gtcaaaatca ttttgtacgg tgttacggca
    15601 ctgtcgacat ttgttctgcc gcaaacacag gcgttaagct ggatagttgg cgtcagcgtt
    15661 ttgctggcaa tgattgggac gtttggtaat gtgtgctggg cgctggcggg gcatctgttt
    15721 cagcgattgt ttcgccagta tggtcgccag ttaaatatcg tgctggcgct gctgctgatc
    15781 tattgcgcgg tacgcatttt ctattaatga aaaaaagcgg aagaggtcgc cctcttccgc
    15841 ttagtaactt gctacttaag ccttacaggc tttcagtaaa ggtacgagcg ataacgtcgc
    15901 gctgctgttc cggagtcaga gagttaaagc gaactgcata accggataca cggatggtca
    15961 gctgcggata tttttccgga tgcttaactg catcttccag agtttcacga cgcagaacgt
    16021 taacgttcag gtgctgacca ccttcaacgc gaacttctgg tttcacttct actggaactt
    16081 cacggtactc aatgtcaccc agtttgctta ctgcaaccac ttcgtcttct gcataacctg
    16141 cttttgcaac gatgcaacgt gcttcgcctt tttcgctgtc cagcagccag aaagagttca
    16201 gcagatcgtc gttagcggct ttagtaatct ggatacctgt aatcatgtga tgcctccccg
    16261 gcaaaattat ttgatttgtt cagcctgtcg cggccaattg gtaaaaccat tgttgcttga
    16321 gtgtatatat actcctcaaa cacccttgaa tctttgattt aaatcaataa aaaccacaca
    16381 tcaagtgtgg tcgcaaatgg attttattgt tttacatcaa cttatgcggg tgtgaaattt
    16441 taccaattta catttttttg cactcgttta agtctaaaaa atgagcatga ttttgttctg
    16501 tagaaagaag cagttaagct aggcggattg aagattcgca ggagagcgag atggctaacg
    16561 aattaacctg gcatgacgtg ctggctgaag agaagcagca accctatttt cttaataccc
    16621 ttcagaccgt cgccagcgag cggcagtccg gcgtcactat ctatccacca caaaaagatg
    16681 tctttaacgc gttccgcttt accgagttgg gtgacgttaa agtggtgatt ctcggccagg
    16741 atccttatca cggaccggga caggcgcatg ggctggcatt ttccgttcgc cccggaatcg
    16801 ccactcctcc gtcactgctg aatatgtata aagagctgga aaatactatt ccgggcttca
    16861 cccgccctaa tcatggttat cttgaaagct gggcgcgtca gggcgttctg ctactcaata
    16921 ctgtgttgac ggtacgcgca ggtcaggcgc attcccacgc cagcctcggc tgggaaacct
    16981 tcaccgataa agtgatcagc ctgattaacc agcatcgcga aggcgtggtg tttttgttgt
    17041 ggggatcgca tgcgcaaaag aaaggggcga ttatagataa gcaacgccat catgtactga
    17101 aagcaccgca tccgtcgccg ctttcggctc atcgtggatt ctttggctgc aaccattttg
    17161 tgctggcaaa tcagtggctg gaacaacatg gcgagacgcc gattgactgg atgccagtat
    17221 taccggcaga gagtgagtaa atttgcggga aaatgccgga tggcagagtt gccacccggc
    17281 tgatttatca ggctttattc tgacgccacc attcgccaag aagaacgccg gttgcgacag
    17341 agatattcag cccggcaacg ttgccagtac catcaatctt cacgcgcaga tcgttcggat
    17401 cgcgtgcggc atccggtaac ccttcatatt cctgacccag caccagtacc attttcgctg
    17461 gcagactggt tttgaacagc ggtttaccct gctcgctgga agttgtcact acggtgtaac
    17521 ccgcctgacg gaaatcatcc agcacgttaa caatgttgtc gccagtaatc ggctgaacgt
    17581 gctctgcgcc gccttctgcg gtacggatag ccgcccccga ctccagcagt gccgcatcct
    17641 gcaccacaac acctttcacg ccaaagtgcg cgcagctgcg catcatgcca cccaggttat
    17701 gcgggttaga ttcgttttcc agtgccagaa cacaatcctg cgcgcctgcc tggcttaccc
    17761 actgctgcac ggttgtgcca ttacgctttt tgatcaggaa gcaaacgcca ccgtgatgtt
    17821 ccgtgcctga cgcttttgtc aattccgctt catctaccac atggtacgct ttgcggtttg
    17881 ctgccatcca gcgcaaggct tctttaaaac gtggcgttac actctggata aaccaggcgc
    17941 gaacaatcgc ttccggacgg ctctggaaca gtgcctgaca ggcgttttcg ccgtagacgc
    18001 gggtttcttc cgcacgctga cgacgcaaca cttccggatc aataaaactt ttaccactga
    18061 tgccaccgtg atcggccttt tccggcgtct catcacccgg cgcgcgggaa accgtgcgcc
    18121 acggcgaatc ttcccacttg cggtcacggg gctgactctg tttgtcatcg cgggcggggc
    18181 gacggccacc gtcagcacga gattttcctg gacgcccgcc ccctttcccg gtacgcgggt
    18241 tgtgggtacg tttatcagaa tcatcatcac tgcggacata catcactttg accttgccgc
    18301 ttttaccttt catttcatcg ttcatgcttt tctccaccag cgctgcgcga agcgcgcaga
    18361 ttacccgaag tccgcgcggt tcgccatgat ttcgtaccaa agcctgcgac tatcatacct
    18421 attgaataaa acagattgtt gtctggaaca atgtccccga taatatgtaa catattagaa
    18481 acataccggc gttgttgccg ataagtctcc ttactcatcc cgaggttagt tatgaatacc
    18541 gtttgtaccc attgtcaggc catcaatcgc attcccgacg atcggatcga agatgcggca
    18601 aaatgcggac gctgcggtca cgacttgttt gacggagagg tgattaatgc gaccggtgaa
    18661 acgctcgaca aattgctgaa agatgatcta ccagtggtga tcgacttctg ggcaccgtgg
    18721 tgcggcccct gccgtaattt cgcaccaatt tttgaagatg tcgcgcaaga gcgtagcggt
    18781 aaagtgcgct ttgtgaaagt gaataccgaa gctgaacgtg aattgagcag tcgctttgga
    18841 attcgtagta taccgacgat catgattttc aaaaacggtc aggttgtcga catgcttaat
    18901 ggcgcagtac cgaaagcgcc gttcgatagc tggctgaacg aatctcttta atcttaccgg
    18961 ggcgcatctt gtgccccgtt ttctcctctg cgacaatggc gttttttcga cgctctctta
    19021 tgaccgaaaa cgctgttctc cagttacgcg ccgagcgtat tgcgcgcgca acacgtcctt
    19081 ttcttgcccg cggtaatcgc gttcgtcgct gccaacgctg tcttttgcca gagaaattat
    19141 gtctctgttc gacaattacc ccagcacaag caaaaagtcg cttctgtttg ctgatgttcg
    19201 acaccgagcc aatgaagccc agtaataccg ggcgtctgat tgctgatatt ttgcctgata
    19261 ccgttgcgtt tcaatggtcg cgtaccgaac cctcgcagga tttgctggat ctggtacaaa
    19321 acccggatta tcagccaatg gtggtctttc ccgcctcgta tgctgatgag caacgggaag
    19381 tgatcttcac accacctgcc ggtaagccac cgctgtttat catgctcgat ggtacctggc
    19441 cggaagctcg caagatgttt cgtaaaagtc cgtatctgga taatcttccc gtcatttccg
    19501 tcgatctttc ccggctttct gcctatcgcc tgcgtgaagc ccaggctgaa ggccaatatt
    19561 gtactgccga ggtagccatc gcactgttag atatggccgg cgataccggg gcggcagcag
    19621 ggttaggcga gcacttcacc cgctttaaaa cgcgctatct ggcaggaaaa acgcaacatc
    19681 tgggtagcat cacagcagaa cagttagaaa gcgtttaaaa tcattcggtc acttctgcgg
    19741 gagaccggta tgagtcagcg aggactggaa gcactactgc gaccaaaatc gatagcggta
    19801 attggcgcgt cgatgaaacc caatcgcgca ggttacctga tgatgcgtaa cctgctggcg
    19861 ggaggcttta acggaccggt actcccggtg acgccagcct ggaaagcggt gttgggtgtg
    19921 ttggcctggc cggatattgc cagcttgccc tttacacccg accttgcggt tttatgtacc
    19981 aatgccagcc gtaatcttgc tcttctggaa gagctcggcg agaaaggctg taaaacctgc
    20041 attattcttt ccgccccggc atcgcaacac gaagatctcc gcgcctgcgc cctgcgccat
    20101 aacatgcgcc tgcttggacc aaacagtctg ggtttactgg ctccctggca aggtctgaat
    20161 gccagctttt cgcctgtgcc gattaaacgc ggcaagctgg cgtttatttc acagtcggct
    20221 gccgtctcca acaccatcct cgactgggcg caacagcgtg agatgggctt ttcctacttt
    20281 attgcgctcg gcgacagcct ggatatcgac gttgatgaat tgcttgacta tctggcacgc
    20341 gacagtaaaa ccagcgccat cctgctctat ctcgaacagt taagcgacgc gcgacgcttt
    20401 gtttcggcgg cccgtagtgc ctcgcgtaat aaaccgattc tggtgattaa aagcggacgt
    20461 agcccggcgg cacagcaact gctcaacacg acggcaggaa tggacccggc atgggatgcg
    20521 gctattcagc gtgccggttt gttgcgggta caggacaccc acgaactgtt ttcggcggta
    20581 gaaaccctta gccatatgcg cccgctgcgt ggcgaccggt tgatgattat cagcaacggt
    20641 gctgcgcctg ccgcgctggc gctggatgcc ttatggtcac gcaatggcaa gctggcaaca
    20701 ctaagcgaag acacctgcca gaaactgcgc gatgcactgc caggacatgt ggcaatctct
    20761 aacccgctcg atctgcgcga tgacgccagc agcgagcatt atgtcaaaac gctggacatt
    20821 ctgctccata gccaggattt tgatgcgctg atggttattc attcgcccag cgccgctgct
    20881 cccgcaacag aaagtgcgca agcattaatt gaagcggtaa agcatcatcc ccgcagcaag
    20941 tatgtttctc tgctgacgaa ctggtgcggc gagcactcct cgcaagaggc acgacgttta
    21001 ttcagcgaag ccgggctgcc gacctaccgt accccggaag gaaccatcac tgcttttatg
    21061 catatggtgg agtaccggcg taatcagaag caactacgcg aaacgccggc gttgcccagc
    21121 aatctgactt ccaataccgc aaaagcgcat cttctgttgc aacaggcgat tgccgaaggg
    21181 gctacgtcgc tcgataccca tgaagttcag cccatcctgc aagcgtatgg catgaacacg
    21241 ctccctacct ggattgccag tgatagcacc gaagcggtgc atattgccga acagattggt
    21301 tatccggtgg cgctgaaatt gcgttcgccg gatattccac ataaatcgga agttcagggc
    21361 gtcatgctgt acctgcgtac agccaatgaa gtccagcaag cggcaaacgc tattttcgat
    21421 cgcgtaaaaa tgacctggcc gcaggcgcgg atccacggtc tgttggtgca aagtatggct
    21481 aaccgtgctg gcgctcagga gttgcgggtt gtggttgagc acgatccggt ttttgggccg
    21541 ttgatcatgc tgggtgaagg cggtgtggag tggcgtcctg aagatcaagc cgtcgtcgca
    21601 ctgccgccgc tgaatatgaa cctggcccgc tatctagtta ttcaggggat caaaagtaaa
    21661 aagattcgtg cgcgcagtgc gctacgccca ttagatgttg caggcttgag ccagcttctg
    21721 gtgcaggttt ccaacttgat tgtcgattgc ccggaaattc agcgtctgga tattcatcct
    21781 ttgctggctt ctggcagtga atttaccgcg ctggatgtca ctctggatat cgcgccgttt
    21841 gaaggcgata acgagagtcg gctggcagtg cgcccttatc cgcatcagct ggaagaatgg
    21901 gtagaattga aaaacggtga acgctgcttg ttccgcccga ttttgccaga agatgagcca
    21961 caacttcagc aattcatttc gcgggtcacc aaagaagatc tttattaccg ctactttagc
    22021 gagatcaacg aatttaccca tgaagattta gccaacatga cgcagatcga ctacgatcgg
    22081 gaaatggcgt ttgtagcggt acgacgaatt gatcaaacgg aagagatcct cggcgtcacg
    22141 cgtgcgatct ccgaccctga taacatcgat gccgaatttg ccgtgctggt tcgctcggat
    22201 ctcaaagggt taggcttggg tcgacgctta atggaaaagt tgattaccta tacgcgagat
    22261 cacggactac aacgtctgaa tggtattacg atgccaaaca atcgtggcat ggtggcgctg
    22321 gcccgcaagc tcgggtttaa cgttgatatc cagctcgaag aggggatcgt tgggcttacg
    22381 ctaaatcttg cccaacgcga ggaatcatga gtaaggtact ggaaatgttg accacttaat
    22441 cgggactggt gttattattg cccgcttatg tcgtctgcat tgcacagagg acccttcaat
    22501 gaacagagaa gaaatgcact gtgatgttgt caaaatttaa gcgtaataaa catcaacaac
    22561 accttgccca actacccaag atttctcaat cagttgatga tgtcgatttc ttttacgctc
    22621 ccgccgactt ccgggagacg ctgctggaaa aaatagccag cgcgaagcag cgcatttgca
    22681 ttgtcgccct gtatctcgaa caggatgacg gtggcaaagg cattctgaac gcgttgtatg
    22741 aagctaaaag gcagcgtccg gaactggatg tgcgggtgct ggtcgactgg catcgtgcac
    22801 aacgtggacg cattggcgct gcggcatcta acactaacgc tgactggtac tgccgcatgg
    22861 cgcaggaaaa tccgggcgta gatgttccgg tttatggcgt tccaatcaat acccgtgaag
    22921 cccttggtgt tctgcacttt aaaggcttta tcatcgacga tagcgtactt tatagcggtg
    22981 ccagcctgaa cgatgtttac ctgcatcagc acgataaata tcgctacgac cgttatcatc
    23041 ttatccgtaa ccgtaagatg tcagacatta tgtttgaatg ggttacacag aatattatga
    23101 atggccgcgg cgttaatcgt ctggatgatg ttaatcggcc aaaaagcccg gaaatcaaga
    23161 acgatattcg tctgttccgc caggagctgc gtgatgccgc ttatcatttc cagggcgatg
    23221 ccgacaacga tcagctttcc gtaacgccgc tagtggggct ggggaaatcg agtctgttga
    23281 acaagaccat tttccatctt atgccttgtg ccgagcagaa actaaccatc tgtacgccat
    23341 acttcaacct gccagcaatc cttgtgcgca atattatcca gttgctgcgc gaagggaaaa
    23401 aggtcgaaat tattgttggt gataaaaccg cgaacgactt ctacattccg gaagatgaac
    23461 ctttcaagat aattggcgca ttgccttatc tctatgagat caatctgcgt cgtttcctga
    23521 gccgtttgca gtattacgtc aatactgacc agctagtggt tcggttatgg aaagatgacg
    23581 acaacaccta tcacctaaaa gggatgtggg ttgatgataa gtggatgttg atcaccggta
    23641 ataacctgaa cccgcgcgcc tggcgtctgg atctggaaaa cgccattttg atccacgatc
    23701 cacaactgga gcttgcgccg cagcgagaga aagaactgga gctgatccgc gagcatacca
    23761 ccatcgttaa gcactatcgc gatctgcaaa gtattgccga ttatccggtg aaggttcgta
    23821 aactcatccg ccgtttgcgc cgtatccgca tcgaccgatt aattagccgc atcctgtaat
    23881 cacaaccccg tcctgtacgg ggtttgtttt ttggaggcca cgttttgcgt attctttttg
    23941 tctgttcgct attgttactt tctggatgca gccatatggc taacgatagc tggagcgggc
    24001 aggataaagc tcaacacttt atcgcctcgg cgatgctttc cgccgccgga aatgaatatt
    24061 cacagcatca ggggatgagc cgggatcgca gtgccatgtt tggattgatg ttctctgtca
    24121 gtttgggagc gtcaaaagag ctttgggata gccgccccga agggagcggc tggagctgga
    24181 aggatttggc ctgggatgtc gccggtgcaa gcaccggcta taccgtctgg caactgaccc
    24241 gtcactaaag acgcatcccc ttccctttgc gatgtagcat caaagaaacc agaaacgcca
    24301 ccacggccat caaggtcaca taccagaaga aggctgtttc cattcctatt gatttcagcg
    24361 acaacgctac gtactccgcc gaaccaccaa atatagcatt agcgaccgca tatgacagac
    24421 caacgcctaa tgcgcgaacc tgtgccggga acatctcagc cttcagtatt ccactgattg
    24481 atgtataaaa actcactatc agcagggcac acatcaccag accaaaagcg gcataaggcg
    24541 aggaaacgtt ttgcaatgct gagagaatag gaacggtaaa aatggctgcc agcgaaccga
    24601 aacataacat tgaggtacgg cgaccaatct tatccgacag cgcgccaatg agtggttgaa
    24661 taagcatgaa tacaaacaat gcggcagtca taatgccact cgccacgttg gcatgcattc
    24721 ccgcagtatt taccagatac ttctgcatat aagtggtgaa ggtatagaaa caaagggagc
    24781 ccgcagcggt aaaaccgaga accatgatga atgcacggcg attgcgccat aatcctttca
    24841 gagatccagc ttcttttaaa gcgcgcgttt cttgttgcga agtttcatct aactgacgac
    24901 gtaaccacaa cgccacaaca gctaacacag ctcctaacgc gaaaggaata cgccatcccc
    24961 actctctgag tgcagcgtct tccatggtgt gttgtaaaac cacgacaacc agtagggcta
    25021 gcagttgtcc gccgatcaac gtcacatact gaaatgatgc gtaaaaacct ttgcgccctt
    25081 caacggcaac ttcactcata taggtggcgc tggtgccata ttctccgcca acggataatc
    25141 cctgaaataa acgcgcgaga agcaataatg ccggagccca cgtaccgatt gtttcataac
    25201 caggcaaaca ggcgattacc agtgagccga aacacatcat acacaccgat agcagcatcg
    25261 attttttgcg accatgttta tcggctatgc ggccaaatag ccaaccgcct attgggcgca
    25321 tcaggaatcc cgcagcaaaa acacctgctg tttgtagtag ttgagtcgtc gtgttcccgg
    25381 aagggaagaa gatgtgggca aagtagagtg aacagaacga gtagacatag aaatcgaacc
    25441 actcgaccag attacctgaa gaggccccca caatcgccca aatgcggcga cgagtatcac
    25501 tacttgtcag tttgctgtct gccgttacag tactttcagc catgccatta tgtctcctgc
    25561 cgtaatccga tgcttttgtc ggtcgcttct gtttatttct tttggtaaag aaaatgttat
    25621 acatttgttg cgtatcatta tgcaacctta accatgaatt tagttagcag gaaaatggtt
    25681 attgaggagc ttaaggataa atttctggta aggaggacac gtatggaagt aggcaagttg
    25741 gggaaaccgt atccgttgct gaatctggca tatgtgggag
//
LOCUS       DAPGOF010000062        25677 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.062, whole genome shotgun sequence.
ACCESSION   DAPGOF010000062 DAPGOF010000000
VERSION     DAPGOF010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25677
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.062"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            137..1459
                     /locus_tag="RHV13_002203"
     CDS             137..1459
                     /locus_tag="RHV13_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HDV1241733.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(1693..3753)
                     /locus_tag="RHV13_002204"
     CDS             complement(1693..3753)
                     /locus_tag="RHV13_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HDV1241734.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(3823..4590)
                     /gene="pdeH"
                     /locus_tag="RHV13_002205"
     CDS             complement(3823..4590)
                     /gene="pdeH"
                     /locus_tag="RHV13_002205"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HDV1241735.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHDLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            4822..5751
                     /gene="kdgK"
                     /locus_tag="RHV13_002206"
     CDS             4822..5751
                     /gene="kdgK"
                     /locus_tag="RHV13_002206"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HDV1241736.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(5847..7343)
                     /locus_tag="RHV13_002207"
     CDS             complement(5847..7343)
                     /locus_tag="RHV13_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDV1241737.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLAITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(7564..8850)
                     /gene="dctA"
                     /locus_tag="RHV13_002208"
     CDS             complement(7564..8850)
                     /gene="dctA"
                     /locus_tag="RHV13_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HDV1241738.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(9033..11021)
                     /gene="hmsP"
                     /locus_tag="RHV13_002209"
     CDS             complement(9033..11021)
                     /gene="hmsP"
                     /locus_tag="RHV13_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HDV1241739.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIERIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(11103..14576)
                     /gene="bcsC"
                     /locus_tag="RHV13_002210"
     CDS             complement(11103..14576)
                     /gene="bcsC"
                     /locus_tag="RHV13_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
                     /protein_id="HDV1241740.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAVQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWSSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDTAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            complement(14558..15664)
                     /gene="bcsZ"
                     /locus_tag="RHV13_002211"
     CDS             complement(14558..15664)
                     /gene="bcsZ"
                     /locus_tag="RHV13_002211"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HDV1241741.1"
                     /translation="MNVLRSGLVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(15671..18010)
                     /gene="bcsB"
                     /locus_tag="RHV13_002212"
     CDS             complement(15671..18010)
                     /gene="bcsB"
                     /locus_tag="RHV13_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HDV1241742.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYIKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(18021..20639)
                     /gene="bcsA"
                     /locus_tag="RHV13_002213"
     CDS             complement(18021..20639)
                     /gene="bcsA"
                     /locus_tag="RHV13_002213"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HDV1241743.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGTSRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            complement(20636..21388)
                     /gene="bcsQ"
                     /locus_tag="RHV13_002214"
     CDS             complement(20636..21388)
                     /gene="bcsQ"
                     /locus_tag="RHV13_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HDV1241744.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKSSGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            complement(21400..21588)
                     /locus_tag="RHV13_002215"
     CDS             complement(21400..21588)
                     /locus_tag="RHV13_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HDV1241745.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDILALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            21861..23432
                     /gene="bcsE"
                     /locus_tag="RHV13_002216"
     CDS             21861..23432
                     /gene="bcsE"
                     /locus_tag="RHV13_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HDV1241746.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            23429..23620
                     /gene="bcsF"
                     /locus_tag="RHV13_002217"
     CDS             23429..23620
                     /gene="bcsF"
                     /locus_tag="RHV13_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HDV1241747.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            23617..25296
                     /gene="bcsG"
                     /locus_tag="RHV13_002218"
     CDS             23617..25296
                     /gene="bcsG"
                     /locus_tag="RHV13_002218"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HDV1241748.1"
                     /translation="MTQFTHNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSIFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQSELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(25382..25489)
                     /locus_tag="RHV13_002219"
     CDS             complement(25382..25489)
                     /locus_tag="RHV13_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDV1241749.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT         5939 a         7163 c         6562 g         6013 t
ORIGIN      
        1 gaagaaaaat cactggccta aacattatcc cctttttgcc tggtttttga ccatttccgc
       61 gatttgttac acattgaaat atcacttttg ctgtgcgtaa tatggctatt cgttagccaa
      121 aaaataagaa aagattatgc aagcaacagc cacaacactc gaccacgagc aagaatacac
      181 gccgatcaac tcgcgtaata aagtccttgt cgcctctctc attggcacgg ccattgagtt
      241 cttcgacttt tatatttacg ccactgcggc cgttattgta tttccgcata tcttcttccc
      301 gcagggcgat cctacggcag caacgctaca gtcgctcgcc accttcgcca tcgccttcgt
      361 cgcacgcccc attggctctg ccgtatttgg tcattttggc gatcgcgttg ggcgtaaagc
      421 gacgctggtc gcctcgttgc taacgatggg gatttcgacc gtggtgattg gtctgctgcc
      481 gggctatgcc acgattggta ttttcgcccc gctgctgctg gcgctggctc gatttggtca
      541 gggtctgggc ttaggcggtg aatggggcgg cgcggcgctg ctggcgactg aaaacgcccc
      601 accgcgcaaa cgtgcactgt atggctcctt cccacagctg ggcgcaccga ttggcttctt
      661 ctttgccaac ggcactttct tgctgctttc ctggctactg accgacgagc agtttatgag
      721 ctggggctgg cgtgtgccat ttatcttctc ggcggtgctg gtcattatcg gcctgtatgt
      781 tcgcgtgtcg ctgcatgagt cgccggtgtt tgagaaagtc gctaaagcga aaaaacaggt
      841 gaagatcccg ctgggtacgc tgctgaccaa acatgttcgc gtaaccgtac tgggtacgtt
      901 cattatgctg gcaacctata cgctgtttta catcatgacg gtctactcta tgaccttcag
      961 taccgccgcc gcgccagttg ggcttggcct gccgcgtaac gaagtgttgt ggatgctgat
     1021 gatggcagtt attggttttg gcgtgatggt gccagtcgcc ggattactgg ctgatgcctt
     1081 tggtcgccgt aaaagcatgg taatcatcac cacgctgatc atcctgttcg cgctgttcgc
     1141 ctttaacccg ctgctcggtt ctggcaaccc gattctggtt tttgccttcc tgctgctggg
     1201 gttaagtctg atgggactga ccttcgggcc gatgggcgcg ctgttaccag agctgttccc
     1261 gacagaagtg cgttacaccg gagcatcgtt ctcttacaac gtagcgtcga ttctcggggc
     1321 ttccgttgca ccatatatcg cagcctggtt gcaggctaac tacgggttag gtgcggtggg
     1381 gttatatctg gcggcgatgg ctggcttgac gttaatcgcc ctgctgctga cccatgagac
     1441 gcgacatcag tcgttgtaag tatcgtgtgg gcagccaccc tcaccccagc cctctccctg
     1501 aaagggagag ggagaaaagc ggagcgagat gttatatcgc gaacataaca gccctccccc
     1561 agaaagtgaa cggaaaagcg gtactcgttg tttcaacacg cacaaaacag tcccctcgcc
     1621 cctttgggga gagggttagg gtgaggggaa cacgctgctc ccgaactcac cagcaaacac
     1681 aatccaacct catcacttct tcatctgcga caatatcgtc ctacactgat tagcctcccc
     1741 ttcggaaggt gagatcagtg ccagtaacgc cgccgccggt gttaccagcg ttgccagtgc
     1801 cgcagcaaca gcaccacggg caatcaacgg cccggctttc accccagcct gcggattttt
     1861 aaacgtccca cgcacataca gcggcgaacg cagtgtgata atccgaatcc ctttactctc
     1921 cggatcaata gtcaaatcca gctgttcgga agcaaaactt gccgtgccgg taacgttaat
     1981 caacgcgttc tcagtatcga aagcaaaaat ctgcgggcgc gccacgccgt tggcaatatt
     2041 cagattcgcc gccgcgcagt tcacccgcac ctcatcatca ccaaatatcg caccgacaat
     2101 gtagttgccg acattcagcc caacaatctc catcaggttg cggctcacca gcccgtcatt
     2161 catcaacagt ttcaggttgc cgttactgtt gcctaaaagt gccgccaccg agttaccgct
     2221 accgcgcagt tctgcgtcac cgttcatttc ccccagcgtc ttctgcatca gttccacatc
     2281 gggcatcagt tctttcagtt tcagtcgacg agcctgaata tctgcccgcc cctgcatcgg
     2341 ctttttatcg ccttccagat gaatattcgc cgcaatgctg ccacccgcca tgccaaattt
     2401 cagcggttgc aggcgcaggt cagcattttt gaggatgata tgagtagaaa gatcgctaat
     2461 cggcaggcta ctgccatgct caatgcgccg ccctttgaag cgaacatcgg catccatgac
     2521 atcccattta tcggtttcga agcggtcata aggcagcact ttgcccgcag gctgaacgct
     2581 tttttcgccc ttcttctgtt cagaccgttt cgacttttct gcccctttcc cggaatcaac
     2641 gccaatcaac ggtcccaggt ccgccagccg caattgccgc gactcgacat caccttccag
     2701 ttttggtcgt ggctttccgg tggtgtagac cagagaaccg tggatatcgc tatcgccaat
     2761 tcgcccatta aaaccgcgat aatcaaagac cgacgatttt tcagtgtcga ttttcgctac
     2821 cagccgacca tccgtttcaa acggcggggt atcgggcagc agaacgcccg tcagttcata
     2881 gagatcaccc agtgaatcgc cagaaaattt aagccgtaaa tcgacaccgc ccatcttcat
     2941 tgggtcattc acgacgccat caaaagcaac acgggtgtta ccagagcgga aatcagcctg
     3001 taccggaaac ggcgtccctt cgccacgcag cgccagcata ccgcctattt ttcccgtacc
     3061 cgtgagcggt tcaccgttat aacgtccctg cgccttcagg ccaaaaacgt aatcgcccac
     3121 cttttcttta tccgctttac ctttcgatcc agtaacttcg ctgaacggca gcggcttgcc
     3181 taacggatca acaaaaatct ccagatccgc tttgcttact ttgtcatcaa tggcgatccg
     3241 cccttgatcg aaaagaatat tatccagccg aaacgaccat gccgacggct ttgcattcgc
     3301 gtctttgttg tcatcgttgg caagattaaa cgtccagtta ttgttctttt cagagaggcg
     3361 aatcaggcgt gcgtcgggct tttccagttt gatccacggt agccagaccg ttttggtcag
     3421 cagcgccaac ggtgccagcg ttgcctctac gcgtggcaaa tgcaccatcg tgacttcggg
     3481 aatatccggt ggattgccaa gaatgatgtc ttccgcgtgt acatggggcc acggcaccca
     3541 gctgcgccag ccagtttctt gtttttgccg ctcccacaca acgcccagat cgccacggat
     3601 agcgaacgga cgattcaact ccgcagagac tttctggttg atggtcggtt tgagtcgatt
     3661 ccagtcaaaa gttgcaatca aaatgatcgc cacgacaatc aacaacaaga aagcccctga
     3721 aatcgcagcg gttattttgc ctgccttgct catcgttcgc ttcctcatat tcttcctgtg
     3781 ccagtcctaa agatagtcca gccaggcgga aaatgaggca gcttatagcg ccagaaccgc
     3841 cgtattcagc gtttctatcg gtgccgggcg tgaaagaaac cagccttgtg cggcgaatgc
     3901 gggcgagttc tgaacatcac gccactcttc cggcgtttct acgccctcga caatcacccc
     3961 gcgacaatag cgattcatta gatgtaaaag ctgagaaaag agtgtgcggc cttccggcga
     4021 ctgacgcagc atcacaaaca gttctcgcgc gattttgatg tagtcataac gcacttcact
     4081 tagtgcagag aaatttgcca tcccggtacc aaaatcatcc agccacagcg ggccaaattc
     4141 acacatcgag gcaaaggttg aatctttcgg cagacggata tgctccacca gttcgaaacg
     4201 cagccaggga agacgctcaa tctggcgcag gatttttggt tgctgacgca gggcgatgag
     4261 cgtagggcca tcaatattga ccgatgccag caggtcgtgc tctacaaaga agtcggcttt
     4321 ttgcgccagc aaatcaatct gctctttcac aacctccatc cgatggctga cggtgatttc
     4381 agtaaaatag cgatccggcg gcaggcgttg cgaagggttc aagggatgcg tgaccaccgt
     4441 taatagctcc acggccatta accgtccgca tgtttgatag atcggctgcc aggtgtaagc
     4501 acgctcacac tgcaaccaaa aacgccgttc ctgcaagctc tcgatgcttg cttcagggtt
     4561 gcttattcgc tggataacct gccttatcat caaagatgtc ctgtgtttaa aagtgatgct
     4621 gcccggactc gacaaagatt atcggcgcgc aagggcagaa ctttaccgcc tggccggggt
     4681 aaatttcctc actccacaac ggaatctttc aggaatacgt tctggctcaa aaaaataatg
     4741 gaacactgtt ttaatatggt tgaccagcaa accaccacag cgcaaactaa cgctaatttt
     4801 ttacagatca ggttcacgac tatgtccaaa aagattgccg tgattggcga atgcatgatt
     4861 gagctttccg agaaaggcgc ggacgttaag cgcggtttcg gcggcgatac cctgaacact
     4921 tccgtctata tcgcccgtca ggtcgatcct gcggcattaa ccgttcatta cgtaacggcg
     4981 ctgggaacgg acagttttag ccagcaaatg ctggacgcct ggcacggcga gaacgttgat
     5041 acttccctga cccaacggat ggaaaaccgt ctgccgggcc tttactacat tgaaaccgac
     5101 agcaccggcg agcgtacgtt ctactactgg cggaacgaag ccgccgccaa attctggctg
     5161 gagagcgagc agtctgcggc gatttgcgaa gagctggcga atttcgatta tctctacctg
     5221 agcgggatta gcctggcgat cttaagcccg accagccgcg aaaagctgct ttccctgctg
     5281 cgcgaatgcc gcgccaaagg cggaaaagtg attttcgaca ataactatcg tccgcgcctg
     5341 tgggccagca aagaagagac acagcaggtg taccagcaaa tgctggaatg cacggatatc
     5401 gccttcctga cgctggacga cgaagacgcg ctgtggggtc aacagccggt ggaagacgtc
     5461 attgcgcgca cccataacgc gggcgtgaaa gaagtggtgg tgaaacgcgg ggcggattct
     5521 tgcctggtgt ccattgctgg tgaagcgtta gttgatgttc cggcggtgaa actgccgaaa
     5581 gaaaaagtga tcgataccac cgcagctggc gactctttca gcgccggtta tctggcggta
     5641 cgtttgacgg gcggcagtgc ggaaaacgcg gcgaaacgcg ggcacctgac cgcaagtacc
     5701 gttattcagt atcgcggcgc gattatcccg cgtgaggcga tgccagcgta aacgcagcat
     5761 atgcaaaagg ctggataaag tgttacccct ctatccagct catccataaa tacctgatgg
     5821 ttgtgctaac tcatcaccat caggcattac tgtgcaggtg gaatatccgt cacttcagga
     5881 tgtacgtcat ccgtggcgac agaggtggtc gcagcggcgg tagatggggc catgatttga
     5941 tcccaggccg cctgcaacgc tttcatgtta aattccggct cgcctttcgg ctgcagcagt
     6001 attaacgcca tatcattcga caactgctga cgcagatcct gatttaacat ctccacagtc
     6061 aggctgttaa ggaaatcctg ccgcagtttc tgatactgct ccggcgcgat atcgacgacc
     6121 tgattttgca acgaacgcat ccgctggccc atcagaatat cggtatcagc ccgtgcatag
     6181 gcggcaaaca gtttctgcag ctccagtttc ttttgcgcca ctaacgcatt gaactcttct
     6241 tccggcagac ctttatcgcg aaccttcgcc agttcacgcg ccaccagatt aaggttgctg
     6301 ttcagcttgt cgtttggtga ttcgatgttg atggcacact gcgcacgcag atacagcaca
     6361 cggcagtcaa atccaagacc gatgtctttg ctgttactgg cgcttaacgc ttgctgaaca
     6421 tgccagaaca gcgcctcgcg ggccaggtcc gcacgccagt agcgcagcag tgcggctgat
     6481 tcacgaatcg gctgccacgg cgtatcccac atgatagata accggtcctg acgcaccgcg
     6541 tcagtcataa tactcaccgc ttccgcacgc agcggagaaa gcgtcggcac cggagctggc
     6601 gtttcacgtt tgcctttcag ttcgccaaac gttttgttta tttggtcgac aaccgagcgc
     6661 gcatccacgt ttcccaccac cagtagcgtc attgcatccg gggtgtacca tttctgatag
     6721 aaatctttaa ttttttccgc ttcaacgggt tgtttcagcg gatcggcagg atcgtgacct
     6781 aacaaggttg accctttcag acgatagcgc caccagccct ctttagtatc ggcaggccaa
     6841 gttgccacca tgtcctgact ttgcagcgcg tggttgatgg tttctggtgt gatggccaat
     6901 ttgccagtgg catttgccag ataagagagc gcttctttca gcaaatcgtt acggttattg
     6961 ggcaaactca gattaaacag cgtggtgtca taagagacaa ttaccggcgg catcgggcgt
     7021 ttagggtcga tcccctgctg ccacaatgaa cgcgcctgtg ctgcgtcaag gccaccgctt
     7081 tgcgttagcg caatacgagg gatggcgtga ctgtaaccgc tctgttgtgt actttcggcg
     7141 agcgaaccgg tattaaccag caggcgaatt tcaacacgat cgctgggacg ctggggggtg
     7201 gtcagcactt gccactgtaa accgttggaa agcgtcccct gttgccatgc tggatcaggc
     7261 tggagcgcat ctgcctgcac atagccagca gtggccatca tcagcaaacc gcccgctaaa
     7321 agtcgaattt ttgtgccctg catgtgaacc cctgatcaac tatcctggta ataaaaaaac
     7381 tgcccgttgt cggtcagtct aaatgacgtt aaaaacactt cgtgttagac cgcaagaaca
     7441 tgaaaatgtc acggaagaag tgaaataaac ctgaactcac ccaggtggta agttcaggca
     7501 caggggtcaa ttatgcgcaa acacccgcac tcggggaagg gagtgcgggc ataagtgatg
     7561 agattaagag gataattcgt gcgttttgcc atccggcgca cgattattca gcacatcgtc
     7621 cagttttttg tggtccagtt ctttcaccca cttagcaacg acaatggtcg ctacgccgtt
     7681 accgaccagg ttagtcagcg cacgagcttc tgacataaag cggtcgatac cgaggatcag
     7741 cgccagaccc gctaccggca aatggcccac cgcagagagc gtcgccgcca gcacgataaa
     7801 gccactaccc gttacccctg ccgccccttt agaagaaagc agcaacacga ttaacagcgt
     7861 gatttggtgg acgatatcca tctgactgtt agtggcttga gcgataaaca ccgccgccat
     7921 tgtcaggtat atcgatgtgc catcaaggtt aaacgagtag cctgtcggga tgaccagccc
     7981 caccaccgat ttacggcagc cgagtttctc catcttgtcg agcatacgcg gcagcgccga
     8041 ctcggaagat gaagtcccca gtacaatcag cagttcttca cggatgtagc ggataaattt
     8101 gaagatactg aaaccagtcg ctttagcgat tgaacccaat accagcacca caaacaggat
     8161 acaggtaatg tagaaacaga taatcagctg ccccagttgc accagtgtgc cgacgccgta
     8221 tttaccgatg gtaaacgcca ttgccccgaa cgcaccaata ggtgccagac gcatgatcat
     8281 attgatgatg ccgaagatga cctgcgagaa actttcgatg acgttaaaaa tcagttggcc
     8341 tttgctgccc agacggtgga gcgcaaaacc aaacagtacg gcaaacagca gcacctgcag
     8401 aatgttaccg ctggcaaatg cgccaatgac gctcgccggg atgacatcca taatgaaggc
     8461 gacaatgccc tggtctttcg cctgatcggc gtaaaccgct accgctttcg catcaagcgt
     8521 tgccggatcg acgttcattc cggcaccagg ctgcacgacg ttaacgatga taagaccaat
     8581 aatcagcgcg atggtactga caatttcaaa gtaaagcagt gcgactgcgc cggtacgacc
     8641 gaccgccttc atgctttcca tgcccgcaat gcccgttacg acggtacaaa agatgacagg
     8701 agcgatgatc atcttaatga gcttaacgaa gccgtcgcca agcggtttca tttgctcgcc
     8761 tatttcagga tagaaatggc caaggagaat accaatggct atcgctgtca ggacctgaaa
     8821 gtaaaggctt ttaaacagag aggttttcat agggtgtcct ttagtaaaac cacaggtctt
     8881 gtaaggttat ggggtacctg cggccttaaa ataacaccca gacaacatca cagaaatgta
     8941 cctggatcat aattgaaaca aaaaggttaa aaagtttgag ctggctcgca caaaccagca
     9001 ctttttaaag ttttgtaatc agtttggggt agctactttt cttccaggta actctcttcg
     9061 aagatttcaa tagggagtgg acgagcaaaa aggaagccct gggcaatacc aacgcccgct
     9121 tttgccagcc agtcgcgttg tgcttcagtc tccacgcctt cggcaatcat ttgtaagttc
     9181 aggctctgcg ccagcatgat aattgcagca atcatgctgc tatcttccgg caagccttca
     9241 acaaacattt tgtcgatttt cagaacgtcg attggcaacg atttcatatg ctgcagctga
     9301 cgcagccctg cgtagcccat gccgaaatca tccagcgcca cccgaactcc agcattgcgc
     9361 agcggacgga ggattgccac cgcagcatga gggtcgtcaa tacgtcggct ttctgtcact
     9421 tccagaatca gtgttcccgg ctgaatgcga tagcgggtta acagttccag catatccgcc
     9481 accatattcg ggtgcatcag ttgcagcgca gagaggttta ccgacaaggg cagcataatg
     9541 ccgcgctctt gccaggctgc aagcaatcga caggactctt ccagcaccca gtgaccgacg
     9601 gtaaccatca gcccacagca ctcaatgcgc tcgattaagc catccggcag gtcccaactg
     9661 ccatccggtt gctggatacg cagtaacact tccgcactga ccagtttacc gctggtcatc
     9721 tcgacctgtg gctgtaacca aatagcaaac tgatgatttt ccagtgcatt aaggatatcg
     9781 ctctcttccg tcaaccgctt ctgggcggct tccatctgct gcggatcaaa gaactgaatc
     9841 tgattcttgc ctttatggcg agcggtaaat gccgcagaaa tagcgcgact gtaaagctgt
     9901 tcggcggtga gatcgccgta gaacatcgcc acgccaatgc tacagtgcgg acggagttga
     9961 atacgttcaa tcggcaggcg ctcgctcatg atagtgagca cttgctgacc taaggtgatt
    10021 gcgtgccacg gttcctgtac accattggca atgacagcaa agtcataacc gctaatctgc
    10081 gcgaggatca tacgtggcga cagtaccgat ttgagttttt ccaccagcgt cagcagcaga
    10141 atttctcgtt gcgcctcttt cagcacaccc gcagtatcac gcagggtttc acaggtgatg
    10201 atcatcagcg cggtggtttg tttacgcgcg acaacctgct ccagcatctc catcagcaag
    10261 gctttgttcg gcaaatccga cactgggaag cgcatcgcat tttcgttctg ttcttcataa
    10321 tggcgctgca gcaattgctg gttgaggttg taactgcgca ccaacatacc gatttcatca
    10381 tcctgatgca gacgcggtaa tgccagttgg tgaccaacaa gctcctgggc tgggatggcg
    10441 ttaagttcgc gagcaatatt gcgtaacgga tgcaaaatca ggcggttaat gcaccagcta
    10501 atcgccaccg tcaatattaa cgacaaaagt aagtaaatgg tcactaacgt tgagagggtg
    10561 ctcatcacga acttatacat acggaaggaa tccgcctgta gcaccagata ggcaattggc
    10621 tgcggatttg ccggacgttc gagcgagtaa acgcccagcg agatttgaac cggtagctca
    10681 aacaggcgag taaccattac cggcaccggg cgctctggaa taaaactttt acgcagcgcc
    10741 tggaactggt tgggcagcac tacatcggca cggctgacca cgcctgccgg tttaatgctg
    10801 gcaagaatag cttccgcttc gggaatatcg cctttcaaaa tagctgaaga taagggttcg
    10861 cggacagagc gagcaatgct ttccagttgc gtagccgtgt tatagcgatt ctgctggacc
    10921 agatggaaca gcaaaacggt gcaaaaaata aaaacgaaca ccaggacaac ggctgccacc
    10981 attgccatct gcttgattgt taacgagcga cttacgcgca aattgtctcc ccccggaaat
    11041 cccagattgc cgcccgagta tacccgatcg cagcggcatt aagagaggcg ctatctgaaa
    11101 acttaccagt cggcgtaagg tatcagcggc tgcggcggta aatccatgtc accctgccat
    11161 ccggcggcgg aataacgtac gtagagcagg aaatggctgg gcgcgtaatc tttcgcctgc
    11221 tggatatcaa ttgccgtgcc aacaaaccag ttggaagtaa cacgtcgttc aagtaatgcc
    11281 cgcgccgtgt agccgaagcc ctgactgctg ccgccatcgt tggattgccg cgcagcttct
    11341 tcctgccagt cggtcgggat cagattcatc agcggataac gcggcatggt tttggtgcgt
    11401 gaatgcgacc acgagccaga cgcccccagc tcccacgacc agttttccgt gcgctcccgc
    11461 cacatcaccg gtatggcaaa cgacaggtat tcctgcggac tgtagtaacc gccctgaccg
    11521 agtgagtagc cactcagatc tttgtcgtaa tgccagatca tgttgttcag gccgattgtg
    11581 acgcggcgat tgttctggtt aatgacctta tagtaatagc ccgtcatcca gcgcacgcgc
    11641 cagttatctt cgacattttt accggttaac tggtcgccac taagcgatgc ccagacgccg
    11701 tttgcttcac ctttatcgta gctcaggctt agccccacgc cgtcggcacg cacgccgccc
    11761 cattttttcc cggtattgct cggggagtct ttttgcccac caaaggccag caaagaactg
    11821 gagatgggac gacggtgggc gttaacggtg taacccagcg gcccgatatc atcgctgtaa
    11881 ctgatgccgc cgaccacatc caccacgttg aagcccatcg gcgtggtgcc gatatcccag
    11941 ctccagacgt catttcgcca gccgaccgcc acgctggcac cggaatccga ctggctgcgg
    12001 ttgccgctac agtcctgtaa tgtacaggtg ccccagttgt catcccattt accatcggca
    12061 ttcgtggaga aactgccgac gttcatattg acgaaatcac tgcgaaagaa catccgcccg
    12121 tcagaatacg gcgcatccac ctgcaacatg gtagtgtgcg ctttcagatc ggagtaacca
    12181 ccggtgccgc tcgaacccca gtaatcatgc tcgagggtga cgttaagatc ctgctggcga
    12241 tagaggtccg ccgcatcgct gcgcacgccg cgtttcagcc agtcatcttt ctcgtcgtta
    12301 cgggtcagtc gggtaaaggt gtcgttatcc tgcggacgcg tcgtagtcac accggatgcg
    12361 accatggcgt ctttgtaggt ttccagcgcc tgctttggat cgcccgcctg cgcttcaaac
    12421 ttcgcaccgt cgcgcagcac catcgcgctt tccatcgacg gtggctgaga ttttgcctgc
    12481 gggatcaact tattaaacgt ctgctgcgcc gctgcggtat cgccaagctg cgcctgcgcc
    12541 agcgccacgc gccgctgtgt gttcagcgag gcgttatcgg tagcgggcag tttcgccagc
    12601 tggctacgtg ccgctgctgt gtcaccggca gcaatatcca cttccgtcaa ccccagaatg
    12661 gcatcggcgt tagttggctc ccgcgtcagg acattctgat atgcagcgcg ggcggcggtg
    12721 taatcacgtc gctgctgcgc ccagtcagcc agcgtgaggt caatacgcgt ggaaggtggt
    12781 tgctgacgca gcatcgcttc cgcttcagct tctttgccgc tttctcgcag gcgattagcg
    12841 gtttccagca cctgatcgct ttgcagtcga ttaaccagct cctgaatatt gctgctccac
    12901 tgcgcacgcg gcaggctgtt gatatgcgcc agcgccgctc tgtcctggtc atgaccagag
    12961 aggtacagcc cgtaagcgta aacctgctcc gggtcgttcg gcttctgctg cgccagattg
    13021 cgcattaacg tatcggcctg gctgcgttgt ccggcctgcc agagatcctg cgaaagtcgg
    13081 taagtaatcc atacgctgcc ggggtccagc gccagtcgtt gccgctgaag tgctgccgcc
    13141 tgcgcccatt tgccctggtt ttccagtgct tctgcctgct gtgccagacg gtcgttttgc
    13201 aggctgcgtt cgatatcatc aatgctacgc cgctgactgg cagagagcga ggcgataaac
    13261 gcttcagctt tttctggcga ttgctggcgg taaatatttg ccagcccgcg cacggcgtta
    13321 gtgttgccgc tgtccatacg caaggtctgc tgataataac gttcggcggc gggataatct
    13381 tttcgcgcca tcgccacatc gcccagcccc agcactgcat aactgtcggt gttatcgaca
    13441 ttacgcgcct gctggaacag gcgttctgcc cggtcaggat tattggcttt cagcgcagca
    13501 tcgccctgct ggatcgccag ccagtagcgg tttactttca gcagactgtt ccatttgtcg
    13561 ttgttgctgc tgtgcgggtc cagtgcgagg gctttttcca gattcgccac tgcattggcg
    13621 cgatcgcctt tctgagaata cgcctggccc agcgccccca gagcttcact gtctttcggg
    13681 ttcgcccgca ccgcctgttg tagttcggga atggctttac ccgccatacc agagtccacc
    13741 gccgctaaac cttgcgcacg agcgcggaaa gcaggatcgg ccagctgttt ttgctgttct
    13801 gccagttgcg attgcacagc cgccacgcta tcgccatcac taaagatcga gagatatttt
    13861 ttcagcgccg acacactggc atcactgacg ggcatgtctt taatctgccc gtaccagatt
    13921 ttagaggccc cttcgcgccc ggcgttcgat tttgccatct gttccaggac ggcaaaacct
    13981 tcgtcacggc gatcgctact aaacagcaat agcgccagat tgttttgcag gcccgtattg
    14041 cccggtgcat ccgcattgat gcgttttaac tgattaatcg cttcgccacg gcgagccgga
    14101 attttcgcca ccgtactcca gtactcgaca gcaatgtcac cttccggcgg cgcaccgttg
    14161 aacagcttgt tgtaactcgc cacagcttct tccgcatgac cggtagtcgc ctgcaatctt
    14221 gcctgttgca gtgcctgacg accatccggc gtggaaagta gcatcgtagt ccgcgacgat
    14281 ttatacgcat ttgaactcgg cgctaactgc gacagccgat cgagctgttt ttgcgcgcca
    14341 tcaatatcgc cctgacgtaa caaagaacgg aaacgggcgg caacgacgtc cgggttattc
    14401 ggatcaataa gttccagccg atataacgac tgctgtacca ggtcttcacg atgggtcgct
    14461 tcgcctaacc gaacttgctc cagcaactgt tgctgagcgg ttggtgctgc ctcgaccatc
    14521 ggcatgacgg ccagaccgag ggaaagcgtg aatatgttta gtgtgaattt gcgcattcct
    14581 ggccccagtc aggtaataac tcaccttttg tcgagaagcg gaaacggtgt tgatcccagc
    14641 cttgtccgaa cagggtcagc acatagttgt aataggcatc gctgccggga aagttatcgg
    14701 ccacgcgctg gcgctgaacg gcctgcgcat cgcggttttg taaaaagggc agcatggcgg
    14761 cagaaaaacc gaccggtcct ttaccctgcg ctttccccgt agccacatcc actttttccg
    14821 gcggataacc gtttttctca gtgaatgtcg ccatcggttt aaaccggttg agcatccgcg
    14881 ctttctgcgg atcgctgtca ggcatcatgc ctacccacat gtaaacgcgg atggcgtcgt
    14941 agctgctgat caatgttttt tcggctttta gctgccagcc tttgtctttc tcataacgca
    15001 cccagtctgg cgaaaagcct ttcggggcgg tttccagcaa taaacgttga ttggtttcgc
    15061 gcagcgtagt ccacggcgcg ccaaagcggg tgaaatactg cgccagcgtc ggcggcaggt
    15121 agctggggtt aaaacgccag ctgttatcct cagcaaaacc cactttgccc ggtaacaaca
    15181 tggaacccag cccaggcacc gtcaccactt cctcccgcgc aatacgtttt agcaacgcgc
    15241 tgccgatgtc ggtataacgc tgctctttcc acaaacgacc cgcctccaac aacgaccagg
    15301 ccatccagac atcaccatcg gaggccgaat tgctgtccag cacttcccac ttactgttct
    15361 ctttcttgcc ccacagccag gcgggcaaac gttcttttaa agaaccctga gcaagattgt
    15421 tctgcgtcca gtcgagaata ttatcgaaag ctgcacggtc gttagccgcc agggcaaaga
    15481 acatgccgta actttgccct tcagaggtgg tgattttgcg cgcgtcgctg gggtcgatga
    15541 cgcgcccttc ctgactgatg taatcctttt taaactgctc ccaggcaggc caggtacagg
    15601 ctgcctgaac actaaaggca gccaacagca gcatcgtcac gagtccacta cgcaacacat
    15661 tcatcttcaa ttactcgtta tccgggttaa gacgacgacg actaataatt cgcagcagac
    15721 gccacagtac ccatgccagc aatatcacac tgatagccgc cagcaccgcc agcagaatcg
    15781 gatggtttgc cagcgcatac cacaagcgct cgaaccacgg cagatgacct acgtaataaa
    15841 cgtcgccaac acgtaggctg ttgataccgg actcgcggat caccgcgacc gaaccgaaca
    15901 tggtggcgcg tttgccgcta tcgttcaccg catcgttaag catttcataa ccgcgtgggc
    15961 tatctgccag cagcgcaatc acgctgcgct ggtcgttata tggcgactgg aagccaatca
    16021 ccgccgccat cgcaccggaa gaggtcagcg ttgactgggt ttctgccgcg cgatcgctct
    16081 cgtccggcac aatgccgggg aacggggtct ggcgcatcgg tgttttcacc cagctttcgg
    16141 tcgcctgcac caataggtcg atctgcttat cgtctttcag tttgtccggg ataccaccga
    16201 tgatcatgat gtcggcatct ttgccctgaa tggtgctgcc atcatcggtc accgtcagat
    16261 taatcgccgg gaagcccgtc tgtgcgccga taaaaccaac agtattcagc aaggtttcca
    16321 tctgtgcttc gttaggcgtt ttcggcatca cggtgatggt ttgcgacaga tccgccatcc
    16381 ggctgaacgg gaagcccgcg ttagcaaagg cgcgtagatc cggcatcggg atgaagtggt
    16441 aatacttcga gaagtcgatg gtggaatcgt caccaatcac cacatgattc tgcaccggct
    16501 ggaaggtaat acagttatcc accgaaccgc ccggcatcgg gttcatatac tcaaagtcga
    16561 agcgcagctg gttggtcgcg cccagtttca gcgccggaat agagacatcc gttttgccat
    16621 ccagcaaacc ttgtaatacc ggaatccgca gcagcaggcg attcgcctcc tgtttgctgc
    16681 tcaggttgaa ggattgcagg aactggttat tcaggctgat atccatccgc gaactgtctt
    16741 tcaccggcgg catggtgtag cggtaattaa tatccatatc aatgccggta ctgcgcatca
    16801 ggtagagatc tggcggcagg tttagcgaaa cattaatcgc tgctggctca agaccgctgg
    16861 attgtaactg ttcttcatag gttttcagtt caccaaaggt gaccggacga tcggtacgca
    16921 cccagttcgg tgcatcgtac ggcttacgcg gtagcagcgg tttcacttca ttcactacca
    16981 cgctttcacc acggaacaga atgttaccct gagcgatacc tttcgctgcc tgcaacaggt
    17041 ctttgtcgtc acgaccaaac accaccagca gtttgatgta aggattctgc ggatggttaa
    17101 tcatctcaat caccggggct tttaccgccg gatgatcgcg caggaagtcc ggccgtttgt
    17161 cgttggttgc aaagacaatg gcattgcgat ccggcaattg gttatagagc accgggaagt
    17221 tctgcccacg ccagccagaa cgcgaaccaa accacgaggc gacaatggca gatgcttgtt
    17281 gcagcccaac atccggcgca cccgcaaaga ccatcggcaa ggtgttagta cggttatcgc
    17341 gcgggtcaaa gaatggcacc gggaagtgtg acaggtcatt cttcacattc agggtctgat
    17401 aggtcagatc cagtccactg ctccgcccaa catccagcca aagcgtggtg ctggccgggt
    17461 tttcgcacac gtcctgataa tggccgacaa actccagccg cacacggttg aagtcggtaa
    17521 taaacagtgg attaatgggc atttgcgcca gcgttttttt acccaactgt tctttggtca
    17581 ctggcagcac gcccatcagt tcatcattga gataaacctt taactgcgac tggacaggca
    17641 gtaacgatgg cgatggcgtg tattcgaggt tgagcatcgc cttcgtcacc acttcatcgc
    17701 tgcgcatacc aaactcaatg ctgccgttcg ggttaatgcc acgtagcacc atgctgcccg
    17761 gcggcggcgc aatttgcgca aaggtcagct tcacatcacg cgaaggaccg ttctgcgcca
    17821 cgactggcgc atcagcgccc tgcacgcccg gcatcacttg ccccacctgc ggattttgtt
    17881 ccgtctgggc ggctacagct ggctcagcat tgatcagtgg ttgcgttgct ggcgtcgcct
    17941 gcgtcatgaa agaggggaac gcactcatcc ccatagccac tgcacaaatc cagaatagtt
    18001 ttcttttcat cgcgttatca tcattgttga gccaaagcct gatccgatgg ttgtgccgtt
    18061 tcgctccgct ccgggcggcg cggaataaac gatacaaccc aggaaaccag agaagtcagc
    18121 acacggaata tgcccttcac cgaagaaggc gcaaactccg ccagatggcg gtagccacgg
    18181 aagccgagct tcagaatatc cagcagactt tccagcggct tatcttccgg atagctgtcc
    18241 tgccagagcg cccatgtatc cgcacgggca aacgtacact gcacaaaatc gatatgttgc
    18301 tgggtggtga gcggcattaa tttcagccca acttcattac ccatcacgcg cgccacctgg
    18361 gtcgggaaga cgtattcctg ctgaccacgt ttaagcaaca gattcacttt ctgcccttcc
    18421 agaatctgcg cctgaccgtt gatcttgatc cccaaaccac cgtcggagaa atcctgaacg
    18481 gtacacgaga agaggtgacc atcttcgcgg gcaattgccg cgggcatcgt catctccacg
    18541 cggtgcgatc ggcgtacctg tttgctttct accgataccg caactgcgcc gccaagaaca
    18601 atcaggttgt agaataccca caccatactg acgaccacgg tgagcatctc ggttggcggg
    18661 ccatagaagt agcgccagat acctaccgca acgcccacca ggttgagcag aacaaggaag
    18721 atgtagggcc gcgagatcac ccagtcgacg tactcttctt ccaccagtcc acctttggcg
    18781 gtgacgttaa atttgccttt gtgcgggtta atcagcgcca ccagcgtcgg tggtgcgata
    18841 taccacgcca gcaccgtttc gtagatttca ctccagaaag agtggcgata tttgccctgg
    18901 atcttggagt tggtcaggct ggcatggatc atatgcggca gtacgaacag ggcgatcatc
    18961 aacgctggcg catagatgat gtaggcatga agcagcagga acgccagcgg cgcagtcagg
    19021 aagatcagcc gtggaatgcc cgacaagaag tggaacatgg cgttgacgta acatagccgc
    19081 tgagcaaact tcagcccttt accggtgagc gggttatcga gacggaagat ttgcaccatc
    19141 ccgcgcgccc agcgaatacg ctgaccgata tgcgccgaca gactttcggt cgccagcccc
    19201 gccgcctgcg gaatacgcat atacgcggag gtatagccac gacggtgcaa acgcagagaa
    19261 gtatgcgcat cttcagtcac agtttcgaca gcaatgccgc caatttcatc cagcggctta
    19321 cggcgaatca ccgcacagga accgcagaag aaagtggcgt cccacatatc gttgccatcc
    19381 tgcaccagac catagaacag cgtgccttcg ttcggtgttt tacgaaaacg ccccaggttg
    19441 cgttcaaacg ggtccggcga gaagaagtgg tgtggagtct gcatcatcgc cagctgtttc
    19501 tctttcagga accagcccat ggtcatttgc aggaacgatc gcgttggtac gtggtcgcag
    19561 tcgaaaatcg acacgaactc gcctttggca tatttcagcg cattgttgat gttgcccgct
    19621 ttcgcatgtt catgagtggt acgggcgata tatttcaccc ccacgttttg cgcaaactgg
    19681 cgaaactctt ccctaccgcc gtcatcgagg atccagatgt tcagcttgtc tttcggccag
    19741 tcgataccca gcgaggcgta aatagtattt ttcaccacgt tgagatcttc gttgtaagtc
    19801 gggacaaaga tatccaccga cggccacagc gacatatctt tcggcaatgg caccggctga
    19861 cgattcagcg gccatactac ctggaagtag ccgagcacca gcacaatcca cgcgtacgtt
    19921 tcagcgaaga gcagaataag cccgcacacc aggctgaccg gatcgtccca gttcagcgta
    19981 gaggtgtaac gccaccagat ataacggcaa gaaacggtca gcgacagcac aatcaacatt
    20041 agcgccgaga agcgccccgg catccgccgt acgatcagcg ctacacccca cagcagcatc
    20101 aggaagataa actgcgccag cgggttaaac ggttgagtaa cgcagattaa cgccagaatc
    20161 agcgagaagg tgacgatgat accgaggatc aaccgccgcg ccccggcact caaatgaccg
    20221 agctcttttt tctcatcaag atgctgtgtt ttatggctaa cgcgctcagg cagctcgttc
    20281 atccattgat ggtaacgtcc acgaatattc tgcagacctg aaaatgcccg cctgcgcggt
    20341 ttcggcgttt ctttgcgcga tgtaccgatc agtaaccagc atgtttgaat gagataacgg
    20401 accgggtcca gcggacgcgg acgcgaggcg ttgatatgcg gatacaggtt tttatgttct
    20461 gcgcgaatac gctgccagcg cggatgctcc agcggaataa aaatccaggc caggatcatc
    20521 cagaaacagc cgagcgtcgc gctgaaagcc gacgcaccgt gacgacgata atcgcgataa
    20581 cgcccgataa gccgcgcgtt gaccggcggg ataagcaacc accgggtcag gatactcatg
    20641 atgcactccc gactggcgtt ttcagcccgg aatagttcaa caggcaccag ttcgccagcg
    20701 tcaatatctc ttcagccgcc agcgcatcac tgcgatattc acctactggt tgcttagccg
    20761 ccaggcattc agccatcgct tcatcacgat gaatgagcat cggcagtaat cggcgctggc
    20821 tttgcaacca aagctggtaa atatcgtcct gaacctgact gccaatacgg aagtcattaa
    20881 tcaaaatatg tgcgccatcc ggcagcgctt gctgatgcag tcggatatgg cagttggcat
    20941 ccacattgac gattgccagc gagtgatcgc acaaactcag cagctggtgg gttatctgcg
    21001 aggcatcacg cggtaagtcg attaaaatcc actggtaacg cccgctggat tttagttgct
    21061 gtaagccgga gcaaatatcg ctcagccggg tttgccagtg ctgtggattt tcttgttctt
    21121 caatggataa ctgaccaaaa ggcagcaaat cgagctgcga ggtgtagcgc aacccagcgt
    21181 cacgccagtc ctggccatcc agcatagctc tggcccagcc ctgacggtgg gtaaaatcaa
    21241 cattaaatga caggcgcaac aagttgtccg ggcaggcatc gaccaccagg acattttctc
    21301 ccaacatttg taatgaccag gctaatgcgg cagtgatggt tgttgtcccc acgcctcccc
    21361 gcaccccctg caatcccagt acggccattc accaatcccc tacttttgtt gcgcaaactc
    21421 tgccagcaac ggccagcgtt ttaatgccgc ggccaactgt tcacgttggg aaatatcggc
    21481 ataatcaata tcaggcagtg aaaatgcctg ctttaacgcc aaaatatcat tctggaagat
    21541 atagcctatc gcgggatcag gcagagtatc tggttcattg ttattcattt tgcttgatcc
    21601 ctatgaaata accgtaccgt tcatgagaca attctaaccg tgagcatttc gccggtttat
    21661 tgtttacggc gagacgggaa tttgctcact ttttgacttt ccgttacgca acagaaaaag
    21721 ttaaaaacga aaagactaaa atcacatgtc ttgtgacctg ctaaattgat tcacatgcta
    21781 aatctgataa gttttaattt caatggtagg tttatttctt agctttcgct agtaaactga
    21841 taatcagaca aaatggtgac atgagggaca ttgtggaccc tgtattctct atcggtatct
    21901 catcattatg ggatgagctg cgacatatgc cagcaggcgg cgtctggtgg tttaacgtcg
    21961 atcgccatga agatgctatc agtctggcga atcaaacaat tgcatcccag gctgaaaccg
    22021 cacacgtcgc ggtcattagc atggacagcg atccggcgaa aatctttcaa ttagatgatt
    22081 ctcaagggcc ggaaaaaata aaattatttt caatgctaaa tcatgaaaaa ggtctatact
    22141 atttggcccg tgatttgcag tgttctattg atccccataa ttaccttttt attcttgttt
    22201 gcgcaaataa cgcatggcaa aacattcctg ccgagcggct gcgctcatgg ttggataaaa
    22261 tgaataaatg gagcaggtta aaccattgtt cgcttttggt aattaatccc ggaaataata
    22321 acgataaaca attttcattg ttgcttgagg aataccgttc actttttggt cttgccagtt
    22381 tgcgttttca gggcgaccaa catttgctgg atattgcctt ctggtgcaac gaaaaagggg
    22441 tcagcgcccg tcagcagctt agcgttcagc aacaaaatgg tatctggaca ttagttcaaa
    22501 gcgaagaggc ggagatccaa ccacgcagcg acgaaaaacg cattctgagt aatgttgctg
    22561 tactggaagg tgcgccgccg ctatcggaac actggcaact gttcaacaat aacgaagtcc
    22621 tgttcaatga agcccgtacc gctcaggcgg cgacggtggt cttttcttta cagcaaaatg
    22681 cgcaaatcga gccactggcc cgcagcattc atactctgcg tcgccagcgc ggtagtgcga
    22741 tgaaaatcct cgtacgggaa aataccgcta gcctgcgcgc caccgatgaa cgtttgttat
    22801 tggcctgcgg tgcaaatatg gttatcccgt ggaatgcgcc actctcccgc tgtctgacga
    22861 tgatcgaaag cgtgcaaggg cagaagttta gtcgctatgt gccggaagat atcactacct
    22921 tgctgtcaat gacccagccg ctcaaactgc gtggtttcca gaagtgggat gtgttctgta
    22981 atgccgtcaa caacatgatg aataaccctc ttttgcctgc ccacggtaaa ggtgttctgg
    23041 ttgccctacg tccggtaccg ggtatccgcg ttgagcaagc cctgacgcta tgtcgcccta
    23101 accgtaccgg cgatatcatg accattggcg gtaatcggct ggtgctgttt ctctcattct
    23161 gtcggattaa cgatctggat accgcgttga atcatatttt cccattgcct actggcgaca
    23221 ttttctccaa ccgtatggtc tggtttgaag atgatcaaat cagtgccgag ctggtgcaga
    23281 tgcgcctgct tgccccagaa caatggggca tgccgctgcc tttaacgcaa agttctaaac
    23341 cggtcatcaa tgccgagcac gatggtcgcc actggcgacg aataccagaa cccatgcgac
    23401 tgttagatga tgctgtggag cgctcatcat gatgaccatc agcgatatca ttgaaattat
    23461 tgtcgtttgc gcactgatat ttttcccgct gggctatctg gcgcggcact ctttgcgacg
    23521 cattcgcgac accttacgtt tgttctttgc taaacctcgt tatgttaaac cggctgggac
    23581 gttacgccgc acggaaaaag ccagggcaac caaaaaatga ctcaatttac gcataatacc
    23641 gccatgcctt cttccctctg gcaatactgg cgcggccttt ccggctggaa cttctatttt
    23701 ctggttaagt tcggcctgtt gtgggcggga tatcttaact tccatccgct cctcaatttg
    23761 gtgtttgccg cgtttctgct gatgcccatt ccgcgctaca gcctgcatcg cttgcgccac
    23821 tggattgccc tgccgatcgg ctttgctttg ttctggcatg acacctggtt gcctggcccg
    23881 gaaagcataa tgagccaggg ttcgcaggtg gcggggttca gtaccgatta tttaatcgac
    23941 cttgtcacac gctttattaa ctggcagatg attggggcca tttttgtttt attggtggcc
    24001 tggttattcc tgtcacaatg gattcgcatt accgtttttg tggttgccat tctgctgtgg
    24061 ctgaacgtac ttaccctggc gggaccaagt ttctccttgt ggccagccgg tcaaccgacg
    24121 accactgtaa caacgacggg tggtaacgca gcggcaaccg ttgcggcgac gggtggcgca
    24181 ccggtagtgg gtgatatgcc cgcacaaact gcaccgccaa caacggcgaa ccttaacgcc
    24241 tggctgaata atttctataa cgcggaggcg aaacgtaaat cgatcttccc gtcttcgctg
    24301 cccgctgatg ctcagccatt tgaactactg gtgattaata tttgttcgct ttcctggtcg
    24361 gatattgaag ccgccgggtt gatgtcgcat ccgctgtggt cgcatttcga tattgagttc
    24421 aagaacttta actccgccac ctcctacagt ggcccggcgg cgatccgttt actgcgcgcc
    24481 agctgcgggc agacttcgca cactaatctg tatcaaccgg caaataacga ctgctatctg
    24541 tttgataacc tgtcgaaact gggctttacc cagcacctga tgatgggaca taacggccag
    24601 ttcggcggtt ttttgaaaga agttcgcgaa aatggcggca tgcagagcga attgatggat
    24661 caaacaaatc tgccggttat tttgctgggc tttgatggtt cgccggttta tgacgatacc
    24721 gccgtgctta accgctggct ggatgttacc gaaaaagata aaaacagccg tagtgccacg
    24781 ttctacaaca cgcttccact gcatgacggc aaccattatc cgggcgtcag caaaacagcg
    24841 gattacaaag cgcgggcgca gaaattcttt gatgaactgg acgccttctt tactgaactg
    24901 gagaaatcgg gtcgtaaagt gatggtggtc gtggtgccgg aacacggtgg cgcgctgaag
    24961 ggcgacagaa tgcaggtatc tggcctacgt gatatcccta gcccgtctat caccgacgtc
    25021 cccgttgggg tgaaattctt cggcatgaag gcaccacatc agggggcacc gattgtcatc
    25081 gaccaaccga gcagcttcct ggctatctcc gatctggtgg ttcgcgttct tgatggcaag
    25141 attttcaccg aagacaatgt tgactggaaa aaactcacca gtgggttgcc acaaacagca
    25201 ccggtctccg agaactcaaa tgcagtagtt attcaatacc aggataaacc gtacgttcgc
    25261 ctgaacggcg gcgactgggt gccttacccg cagtaagaac gaaaaaggcc gcagagtttc
    25321 acccctgcgg cctggtccgg ggcaaattgc cattacggca gcctgacgcc cgcatgacac
    25381 gttacttccg tttgttcagc cagttcacga tcatactggc aagaatgcca gcaatgaccg
    25441 gagccgctaa atcatgccag aaggccatgc ccagctctgc gagcgtcata tagccgcctg
    25501 tgttgtaatg acaacgtttc gcggctattc ttgtattgtt tggatgcaag aatggccccc
    25561 gtgatgttgt caggtgcata cctgcaacgt gcgggggttt tctctctcca gcaaccaatg
    25621 ccaccaggga ttaagccccc gcaacattgc gcctcaccgg aacccttccg gcttgcc
//
LOCUS       DAPGOF010000063        24875 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.063, whole genome shotgun sequence.
ACCESSION   DAPGOF010000063 DAPGOF010000000
VERSION     DAPGOF010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24875
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.063"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            218..1654
                     /gene="mdtQ"
                     /locus_tag="RHV13_002220"
     CDS             218..1654
                     /gene="mdtQ"
                     /locus_tag="RHV13_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HDV1241750.1"
                     /translation="MNRDSFYPAIVCFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            1707..2468
                     /locus_tag="RHV13_002221"
     CDS             1707..2468
                     /locus_tag="RHV13_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV1241751.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(2598..3176)
                     /locus_tag="RHV13_002222"
     CDS             complement(2598..3176)
                     /locus_tag="RHV13_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDV1241752.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            3346..3933
                     /locus_tag="RHV13_002223"
     CDS             3346..3933
                     /locus_tag="RHV13_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HDV1241753.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            4107..5039
                     /gene="pbpG"
                     /locus_tag="RHV13_002224"
     CDS             4107..5039
                     /gene="pbpG"
                     /locus_tag="RHV13_002224"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HDV1241754.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKMLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(5077..6792)
                     /gene="dld"
                     /locus_tag="RHV13_002225"
     CDS             complement(5077..6792)
                     /gene="dld"
                     /locus_tag="RHV13_002225"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDV1241755.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            6988..9285
                     /gene="bglX"
                     /locus_tag="RHV13_002226"
     CDS             6988..9285
                     /gene="bglX"
                     /locus_tag="RHV13_002226"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HDV1241756.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKHE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGKFELL"
     gene            9536..10453
                     /gene="osmF"
                     /locus_tag="RHV13_002227"
     CDS             9536..10453
                     /gene="osmF"
                     /locus_tag="RHV13_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HDV1241757.1"
                     /translation="MPLSKVWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDEKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            10460..11617
                     /gene="yehY"
                     /locus_tag="RHV13_002228"
     CDS             10460..11617
                     /gene="yehY"
                     /locus_tag="RHV13_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HDV1241758.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAGLVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            11610..12536
                     /gene="yehX"
                     /locus_tag="RHV13_002229"
     CDS             11610..12536
                     /gene="yehX"
                     /locus_tag="RHV13_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HDV1241759.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGEIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPSGTLHFQDLLVEA"
     gene            12541..13272
                     /gene="yehW"
                     /locus_tag="RHV13_002230"
     CDS             12541..13272
                     /gene="yehW"
                     /locus_tag="RHV13_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HDV1241760.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(13253..13360)
                     /locus_tag="RHV13_002231"
     CDS             complement(13253..13360)
                     /locus_tag="RHV13_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HDV1241761.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(13420..14151)
                     /gene="mlrA"
                     /locus_tag="RHV13_002232"
     CDS             complement(13420..14151)
                     /gene="mlrA"
                     /locus_tag="RHV13_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HDV1241762.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIHDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGYDIFPLGI"
     gene            14373..16058
                     /gene="btsS"
                     /locus_tag="RHV13_002233"
     CDS             14373..16058
                     /gene="btsS"
                     /locus_tag="RHV13_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HDV1241763.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            16055..16774
                     /gene="yehT"
                     /locus_tag="RHV13_002234"
     CDS             16055..16774
                     /gene="yehT"
                     /locus_tag="RHV13_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HDV1241764.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            16821..17291
                     /locus_tag="RHV13_002235"
     CDS             16821..17291
                     /locus_tag="RHV13_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HDV1241765.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(17332..17793)
                     /locus_tag="RHV13_002236"
     CDS             complement(17332..17793)
                     /locus_tag="RHV13_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HDV1241766.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(17918..19921)
                     /locus_tag="RHV13_002237"
     CDS             complement(17918..19921)
                     /locus_tag="RHV13_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HDV1241767.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     DALIATFSDGVRTQLANGQALKEAQCSCGANGMCRHRVMLVLSYQRLCATTQSTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCTPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSNDPFANDEG
                     NACRQYVQQLSQALWLGGISQPLIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGAGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAARRSAEGELLFATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNQLTLSL
                     PASASAPYAVERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVASLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAIGQAELLANDLTA
                     VGFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(19918..21054)
                     /locus_tag="RHV13_002238"
     CDS             complement(19918..21054)
                     /locus_tag="RHV13_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDV1241768.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARQIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            complement(21047..23326)
                     /locus_tag="RHV13_002239"
     CDS             complement(21047..23326)
                     /locus_tag="RHV13_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1241769.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDFPGDALNRQREAFMARWIAWAVQQNNGDVLVVCGGWHAPALAKMWRECPQDIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQSWCWQWGLQQAGEQLLKT
                     VLTRLRQHHLPASTADMAAAHLHSMALAQLRGHKLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNSEERWKLTRPLSQHAAL
                     IEAVCFGATLQEAARHKLEADMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVNLSGVSLSAAVALLERHSQANHAPALDRGAVLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFNSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            complement(23337..24425)
                     /locus_tag="RHV13_002240"
     CDS             complement(23337..24425)
                     /locus_tag="RHV13_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDV1241770.1"
                     /translation="MSPQNNHLQRPPAAVLHADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDNAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT         5809 a         6352 c         6775 g         5939 t
ORIGIN      
        1 tcctttattt gtgagtgctg aataagtata ggaaaaaacg aagcccttcg ctgtcattta
       61 taagtgatta agataaatcg gagaatatca ttcacttagt gtatattttt ctggatatgt
      121 cgttttattg ctactgatgt attgccacgc gaaactaaaa aatatatcgc aacacacggt
      181 aatctccgcg caccttttct tacctgatta ttcccccatg aatcgtgatt ccttttatcc
      241 agccatcgtc tgttttccgc tgttactgat gctggccggg tgtgcgccta tgcatgaaac
      301 ccgccaggcg ttaagccagc aaacgcccgc tgcacaagtt gacaccgcat tacccacggc
      361 gctgaaaaat ggttggccag acagccaatg gtggctggag tatcacgata atcaactcac
      421 ttcattaatt aacaatgcac tacagaacgc accggatatg caggtggctg aacagcgcat
      481 tcagctagct gaagcccagg cgaaggcagt tgccactcag gatggtccgc agatcgactt
      541 ttcggcggat atggagcggc aaaaaatgtc ggcagaaggc ttaatggggc cgtttgctct
      601 gaacgatccg gccgcaggta cgaccggccc gtggtacacc aacggtactt ttggcttaac
      661 ggcgggctgg catctcgata tctggggaaa gaatcgggcg gaggttactg cccgcctggg
      721 tacggttaaa gcacgggcgg cggaacgcga gcaaacccgc caattgctgg ctggcagcgt
      781 agcccgcctg tactgggagt ggcaaaccca ggcggcgtta aacacggtct tgcagcaaat
      841 agaaaaagag cagaacacca ttatcgcgac cgatcgccag ctatatcaga acgggattac
      901 ttcttcagtt gaaggtgtgg aaaccgatat taatgccagc aaaacccggc agcagctcaa
      961 cgatgtcgcg gggaaaatga aaattattga ggcacggtta agcgcactta caaataacca
     1021 gacaaagtca ttgaagctta aaccggtcgc gttgccgaaa gtggcaagcc agcttcctga
     1081 tgaactgggg tactccttac tggcccggcg ggcagatttg caggcggcgc actggtacgt
     1141 tgagtcatcg ctaagcacca ttgatgcggc aaaagcggca ttttatcctg acatcaacct
     1201 gatggccttc ctgcaacagg atgcgttgca cttaagcgat ctgttccgtc attccgcgca
     1261 gcaaatgggc gttacggcag gcctgacgct acccattttc gatagtggtc gtcttaacgc
     1321 caatctcgat atcgcaaaag ccgaaagcaa cttgtctatc gccagctaca acaaagcggt
     1381 ggttgaagcg gtgaatgacg tggcgcgggc agccagtcag gttcagacac ttgcggagaa
     1441 aaaccagcat caggcgcaaa ttgagcgcga tgccttgcgt gtggtaggtc ttgcgcaggc
     1501 gcgctttaac gcgggcatca ttgctggttc ccgcgtcagc gaagccagaa tccccgcgct
     1561 gcgtgagcgg gccaatggcc tgttattgca agggcagtgg ctggatgctt ccattcaact
     1621 cactggtgcg ttgggcgggg gttacaaacg ctgatgatat gcgccttcta tacttaacgt
     1681 ttattcagcg ttaagtggag aactcgatgg cacaggttgc gattattacc gcctccgatt
     1741 cggggatcgg caaagagtgc gcgttattac tggcgcagca agggtttgat attggcatta
     1801 cctggcactc agatgaagaa ggggcaaaag ataccgcgcg tgaggtagtt agccacggcg
     1861 tacgtgcgga gatcgtgcag ctggatctcg gtaaactgcc agaaggtgcg caggcgctgg
     1921 agaaactcat tcaacggctg gggcgtattg atgtgctggt gaataatgcg ggcgcaatga
     1981 ccaaagcgcc gtttcttgat atggctttcg atgagtggcg caagattttt accgttgatg
     2041 tcgatggtgc atttttatgc tcacaaattg cggcccgcca gatggtgaaa caagggcagg
     2101 gcggtcgcat catcaacatt acgtcggtac atgaacatac gccgctgccg gatgccagcg
     2161 cttacacagc cgctaaacat gcgctcggcg ggttaaccaa aacgatggcg ctggagctgg
     2221 tcaggcataa gattttggtg aacgcagtcg cgcctggggc gatcgccacg ccaatgaatg
     2281 gcatggatga cagcgacgtg aagcccgacg cggagccttc gattcctctg cggcgttttg
     2341 gcgcaacgca tgagattgcc agcctggtag cgtggctgtg ctcagagggc gcaaattaca
     2401 ccaccgggca gtcgttgata gtggatggcg gctttatgtt ggcgaatccg cagttcaacc
     2461 cagaatagcg cagtgaggtg cgtggttatt tcaatgggta aaattttggt aggcatgata
     2521 agacgcacaa gcgtcgcatc aggcatggtg caccggatgt cggatgcggc gcgaacgcct
     2581 tacccagggc aatggtttta cgcctgatga tccggctttt tcttcccgcg ccgtttcagc
     2641 caccagcgca cgcccaccac cagaaccacg accagaatca accagaccca gtgcttcaaa
     2701 tgctggtcga gattgtgcaa ccacggcgca atcacctgac caccagcgta accaatagtg
     2761 gtaaaaatca acgcccaggc aaatgcgccg agaatattca gcggcagaaa gattttcggc
     2821 ggcagctggc tggcaccaat cagcgtcggg ccaatcaccc gaaagccata cataaagcgc
     2881 gtaccaatga caaacagata cggatggcgt tggataagtt tctgcgcccg ctcaatttta
     2941 tcctgatgtt tcgagaaacg gcgtaacagc ttgccgccaa accgccgccc gcacagatag
     3001 agcacctggt cgccaatcat gccgccaagc gccacagaaa gtaccaccag cgggaacttt
     3061 aatagcccct gatgcgccgc aacgcctccc agcaaagtca cggtttcacc ttccgccagg
     3121 ctaccgatca ccagcgcggc ataaccatat tgtgagataa gtgtattgag atccataggc
     3181 cagtctgtct ccctttggtt gccagtaagc atacaccctg tgaatcattc acgctgaaaa
     3241 aggacgttca tcagacagtg aaaattacat gggcgaacaa aaaataatca caagctgact
     3301 tatacttgaa ctgtcaggtt catgaactca caacaagggg gcggtatgag ccatgtctgg
     3361 ggactgtttt cccatcccga tcgtgaaatg caggtgatta atcgcgaaaa cgaaacgatt
     3421 tctcaccatt acacccacca cgttttgctg atggcggcga ttccggtgat ttgcgccttc
     3481 attggcacta cacagattgg ctggaatttt ggcgatggca ctatcctgaa gttatcctgg
     3541 tttaccggac tggcgctggc tgtcttgttt tacggcgtta tgctggctgg ggtcgcggtg
     3601 atggggcggg tcatctggtg gatggcgcgc aattacccgc agcgtccgtc acttgcgcac
     3661 tgcatggtct tcgcgggcta cgttgcaact ccgctgtttt taagcggtct ggtggcgctt
     3721 tatccgctgg tctggttgtg tgcgctggtc ggcacggtgg cgctgtttta caccggctac
     3781 ctgctttatc tcggtattcc gagcttcctg aatatcaaca aagaggaagg ccttagcttc
     3841 tccagttcga cgctggcgat tggcgtgctg gtactggaag tgctgctcgc ccttaccgtt
     3901 atactatggg gctacggtta tcgtttgttc tagaaagttc tttgacgttg cattgctggc
     3961 gtaaatgtca gcaatgcagc attttctcac gattctcctt tgacgatcgc actttttgct
     4021 cgttatgatg gcgtcgctag cctcagtaaa tccttactcc ggcggtgcgc aacccgtgcg
     4081 cgtgaaccac tatctgaatg ctcatcatgc cgaaatttcg agtttcttta tttagcctgg
     4141 ccctgatgct ggctgtgcct ttggcaccgc aggccgttgc gaaaacggca gccgctacca
     4201 ccgcttcaca accggaaatt gcctccggta gcgcgatgat tgttgatctg aataccaaca
     4261 aagtgatcta ttcgaaccac ccggatctgg tgcgtccgat tgcgtctatc agcaaattaa
     4321 tgaccgcgat ggttgtgctg gatgcacgac tgccgctgga tgaaaaacta aaagtggata
     4381 tcagccagac gccggagatg aaaggggtct attcgcgcgt acgactgaat agcgaaatca
     4441 gccgtaaaga tatgctgttg ctggcgctga tgtcttcaga aaaccgcgcg gcggcaagcc
     4501 ttgcgcacca ttatcccggt ggttacaaag cctttattaa ggcaatgaat gcgaaagcga
     4561 aatcgctcgg aatgaacaac acgcgctttg ttgaacctac cggattgtcg gtgcataacg
     4621 tttcaactgc ccgtgactta accaaaatgc tcattgccag caaacagtat ccgttgatcg
     4681 ggcagttaag tactacccgg gaagatatgg caaccttctc taacccgacg tatacgctgc
     4741 cgttccgcaa tactaatcat ctggtgtatc gcgataactg gaatattcag ttaaccaaaa
     4801 ccggctttac caatgcggcg ggccattgtc tggtgatgcg tacggttatc aataataaac
     4861 cggtggcgct ggtagtgatg gacgcgtttg gcaaatatac ccattttgcc gatgccagcc
     4921 gcctgcgtac ctggattgaa accggtaaag tgatgcctgt gccggcagca gcgttgagct
     4981 ataaaaaaca aaaagccgcc caaatggcgg cggcggggca gacggcacag aacgattaag
     5041 tgaattcgga tggcgatact ctgccatccg taatttttac tccacttcct gccagttttt
     5101 ccgtttactg gttttaccga tccccggatt catgctgttg gtcggatcgt tctcgcgata
     5161 gaacttctgc aacgtctccg gtgctttata caaatgaccg acgttatgct cggcagggta
     5221 ttgcgcgccg cgctgctgta gcagttccag catctgttct tttaacgcat gcacatccac
     5281 gccttttttc actatgtaat cctgatggaa gacatagcac ataaaatggc cgtaatagag
     5341 cttgtgcacc agctggctgt cgatctccgg cggtaaatgc tcataccact cggtgtcgtt
     5401 acgccgcaga gcgatatcca acgccagaat gtcttcgact tcatcggaat gcaccgcctg
     5461 ataacgaatt gctgcgcccg cagcggcgaa acggtgtaaa aacgctttgc tgccttcctc
     5521 cggcgtacag acaaagaaat cgccttcggc ctgtttgaaa taatccacca accacgattt
     5581 ggcttcgccc acgccatcgc ccgccatttt taacagcaga tgatgctcgt atttatcgcg
     5641 ccagtttttc atgcgcggcg gtaaatggct ggggaacagg tgaccgaatt tttgcatcgc
     5701 acggtcagta aaatgcggac ggaagaattt cactttctcc agcatcgcat cggtgcgtcc
     5761 cttgagatta aagaagaacg gcatcttgtc ggtgcctaac ttatcaatca tcaggaaggt
     5821 gtctttgccg tatttttccg caatatcgta gatatcccgg tgcatatatt ccccggcaac
     5881 cggcagattt tcgaagttag ccagaatatg acggcggatt tcggtcagca cttccggctg
     5941 gttggtgccg atataaaaca cctgctgatt tttttccgct tcgaaggtat ctagacgtac
     6001 cgcaaagacg gccagcttcc cggcgcaacc agaagattca aataaccgat ccggatcggc
     6061 gttataacgt gcgggcgtgt cggcttcaat atcacgaacg cggtggacat agtcataatc
     6121 gtgggcgtga cgaccatcgt gacgcacatc gtcatctttg atgcgatcat catccagctt
     6181 gctaaggatc tgctccggcg tttcgcccag atcaatcccc agatggttca ccagcgtcag
     6241 tttgccgtct tcatttatac gcgcgaataa cgacatttcg gtatacgccg ggccacgttg
     6301 caccagcgag ccgccggagt tgttacaaat accgccgata accgatgcgc ctatacacga
     6361 cgatccaatc accgagtgcg gttcgcgtcc cagcggtttg agggcttttt ccagcgaata
     6421 gagtgtggtg cccggatagg ccagcacctg ttcgcccttg ccaagaacgt gcagcttgtc
     6481 gagacgcagg gtgctgatga tcacgatatc gcgatcatag tcgttaccgt ttggcgtcga
     6541 tccttcggtc aggcctgtat tggcagcctg catcagaata attttgtcgg cggtgacaca
     6601 ggctttcagc acccgccaca attccagtag tgagccaggg aaaacgacag ccagcgcgtc
     6661 gccctgacca gaacggaagc ccttgcgata gcgggccgtt tttgcgggat cggtgagcag
     6721 gtgtgaatga ccgaccagac gggcaagttc attcaaaaag gctttattat cagttgttgt
     6781 catggaagac attttccact ccttgtggtg gcgaaaaaat atcacaaact agaatagcgc
     6841 cttgtatgac aattcagcga gtgaatattt tgaaaattca gacaataaac tgagcattct
     6901 ttttctctct ccatcatgct tatggcaaac tacggttttt gcaccatttt cgctgcctcg
     6961 tcgggcaaca gaggaagaaa aatccatatg aaatggctat gttcagtagg aatcgcggtg
     7021 agtctggccc tgcagccagc actggcggat gatttattcg gcaaccatcc attaacgccc
     7081 gaagcgcggg atgcgttcgt caccgaactg cttaagaaaa tgacagttga tgagaaaatt
     7141 ggtcagctgc gcttaatcag cgtcggcccg gataacccga aagaggcgat ccgcgagatg
     7201 atcaaagacg gtcaggttgg ggcgattttc aacaccgtaa cccgtcagga tatccgcgcc
     7261 atgcaggatc aggtgatgga attaagccgc ctgaaaattc ctcttttctt tgcttacgac
     7321 gtgctacacg gtcagcgcac ggtgttcccg attagcctcg gtctggcctc gtcttttaac
     7381 ctcgatgcag tgaaaacggt cggacgtgtc tctgcttatg aagcggcaga tgatggcctg
     7441 aatatgacct gggcaccgat ggtcgatgtc tcgcgcgatc cgcgctgggg acgtgcttcc
     7501 gaaggttttg gcgaagatac gtatctcacc tcaacaatgg gtaaaaccat ggtggaagcg
     7561 atgcagggta aaagcccggc agatcgctac tcggtgatga ccagcgtcaa acactttgcc
     7621 gcatacggcg cggtagaagg cggtaaagag tacaacaccg tcgatatgag tccgcagcgc
     7681 ctgtttaatg attatatgcc gccgtacaaa gcggggctgg acgcaggcag cggcgcggtg
     7741 atggtggcgc tgaactcgct gaacggcacg ccagccacct ccgattcctg gctgctgaaa
     7801 gatgttctgc gcgaccagtg gggctttaaa ggcatcaccg tttccgatca cggtgcaatc
     7861 aaagagctga ttaaacatgg cacggcggca gacccggaag atgcggtgcg cgtggcgctg
     7921 aaatccggaa tcaacatgag tatgagcgac gagtattact cgaagtatct gcctgggttg
     7981 atcaaatccg gcaaagtgac gatggaagag ctggacgatg ctgcccgcca tgtactgaac
     8041 gttaaatatg atatgggatt gtttaacgac ccatacagcc atttggggcc gaaagagtct
     8101 gacccggtgg ataccaatgc cgaaagccgc ctgcaccgta aagaagcgcg tgaagtggcg
     8161 cgcgaaagcc tggtgttgct gaaaaaccgt ctcgaaacgt taccgctgaa aaaatcggcc
     8221 accattgcgg tggttgggcc actggcggac agtaaacgtg acgtgatggg cagctggtcc
     8281 gcagccggtg ttgccgatca atccgtgacc gtactgaccg ggattaaaaa ttccgtcggt
     8341 gaaaacggta aagtgctgta tgccaaaggg gcgaacgtta ccagtgacaa aggcattatc
     8401 gatttcctga atcagtatga agaagcggtc aaagtcgatc cgcgttcgcc gcaagagatg
     8461 attgatgaag cggtgcagac ggcgaaacaa tctgatgtgg tggtggctgt agtcggtgaa
     8521 gcacagggga tggcgcacga agcctccagc cggaccgata tcactattcc gcaaagccaa
     8581 cgtgacttga ttgcggcgct gaaagccacc ggtaaaccgc tggtgctggt gctgatgaac
     8641 gggcgtccgc tggcgctggt gaaagaagat cagcaggctg atgcgattct ggaaacctgg
     8701 tttgcgggga ctgaaggcgg taatgcaatt gccgatgtat tgtttggcga ttacaacccg
     8761 tccggcaagc tgccaatgtc cttcccgcgt tctgtcgggc agatcccggt gtactacagc
     8821 catctgaata ccggtcgccc gtataatgcc gacaagccga acaaatacac ttcgcgttat
     8881 tttgatgaag ctaacggggc gttgtatccg ttcggctatg ggctgagcta caccactttc
     8941 accgtctctg atgtgaaact ttctgcgccg accatgaagc gtgacggcaa agtgactgcc
     9001 agcgtgcagg tgacgaacac cggtaagcac gagggggcca cggtagtgca gatgtacttg
     9061 caggatgtga cggcttccat gagtcgccct gtgaaacagc tgaaaggctt tgagaaaatc
     9121 accctgaaac cgggcgaaac tcagactgtc agcttcccga tcgatattga ggcgctgaag
     9181 ttctggaatc aacagatgaa atatgacgcc gagcctggca agttcaatgt atttatcggc
     9241 actgattccg cacgcgttaa gaaaggcaag tttgagttgc tgtaatgctt tagcaagggc
     9301 gcgacgtttg cgccctttgc ctgatttcat atcttaactt gctgaatttt cgccctttgt
     9361 aggccggata aggcgctcgc gccgcatccg gcaggaaacg ccgggcaaaa tatgcccttt
     9421 aagtatttat ctgattctgc aactgaatcc ttccgctcag gctaatcccg ccattatcaa
     9481 ctatgctttt ctcttaattc gctgaaaaca acagtaaaag aatgaggaaa gcatcatgcc
     9541 actctcaaag gtctgggcag gttcactggt tatgttggca gccgtgagcc tgccgctaca
     9601 ggcggcttcc cccgttaaag tcggttcaaa aatcgatacc gaaggcgcgc tactcggcaa
     9661 catcatcttg caggtgctgg aaagccacgg cgtaccgacg gtgaataaag tgcaactcgg
     9721 aacgactcct gtggtgcgcg gggcgattac ttccggtgaa ctggatatct atccggaata
     9781 taccggtaat ggcgctttct tctttaaaga tgaaaacgat gcggcatgga aaaacgcgca
     9841 gcaaggttac gagaaagtca aaaaactcga tgcagagcaa aacaagttaa tctggctgac
     9901 gcccgcacca gcgaataaca cctggaccat cgccgtgcgt caggatgtgg cagagaaaaa
     9961 caaactcact tcgcttgccg acctgagtcg ttatctgaaa gagggcggca ccttcaaact
    10021 ggcagcctcg gcagagttta tcgaacgcgc cgatgcgtta cccgcgtttg aaaaagccta
    10081 cgactttaaa ctcgatcagg atcagttact gtcactggct ggcggcgaca cggcggtaac
    10141 gattaaagcc gctgcccagc aaacttctgg cgttaatgcc gcaatggctt acggcactga
    10201 cggtccggtc gcggcgctgg ggctgcaaac cttaagcgat ccgcaaggcg tgcaacctat
    10261 ctacgcgcct gcaccagtgg tgcgtgagtc ggtgttgaaa gagtatccgc aaatggcaca
    10321 gtggctacag ccagtcttcg ccagcctcga tgaaaaaaca ttgcagcaac tgaatgccag
    10381 cattgcagtg gaaggactgg atgccaaaaa agtggctgcc gactacctga aacaaaaagg
    10441 gtggacgaag taatttcccg tgacttatct acgtattaat cctgttctgg cgctgctgct
    10501 gttgctgacg gcaatcgcag cggcgctgcc gtttatcagt tacgcgccta atcgtttagt
    10561 ctcgggtgaa gggcgtcatc tctggcaact gtggccgcaa acgatctgga tgctggtggg
    10621 cgttggttgc gcctggctga cagcctgttt tattcccgct aaaaaaggca gcatttttgc
    10681 actcattctg gcgcaattcg tcttcgtatt gctggtgtgg ggagcaggaa aggcggcgac
    10741 ccaactggct caaaatggca gtgcgctggc gcgtaccagc ctcggcagtg gtttctggct
    10801 ggccgcggcg ctggcattgc tggcctgtag tgatgccatc cgccgaatct ccacgcatcc
    10861 gctgtggcgc tggttgttgc atatgcagat tgccattatt ccgctgtggt tgctgtactc
    10921 cggcacgctt aacgatctct cactaatgaa agaatacgcc aaccgtcagg atgtgtttga
    10981 cgacgcgctg gcacaacatc tgacgttgct gtttggtgcg gtgctgcctg cgttagtgat
    11041 tggtgtgccg ttgggcatct ggtgctactt ttccaccgct cggcaggggg cgattttttc
    11101 tctgctcaat gtcattcaga ccgtgccttc ggttgcgctc tttggcctgc tgattgcgcc
    11161 gcttgccgga ctggttacgg cctttccgtg gctggggaag cttggcatag caggaaccgg
    11221 aatgacaccc gcactgattg cgctggtgct ctatgccttg ctgccgctgg tgcgcggcgt
    11281 ggtagtcggc ttgaaccaga tcccgcgcga tgtgttggag agcgccagag cgatgggcat
    11341 gagcggagcg cagcgattcc tgcatgttca gttaccgctg gcgttaccgg tatttctgcg
    11401 cagcctgcgg gtggtgatgg tgcaaactgt aggcatggcg gtgattgcgg cgttaatcgg
    11461 cgcaggcggt tttggtgcgc tggttttcca ggggctgcta agcagcgcca ttgatttagt
    11521 gttgctgggg gtgatcccgg taattgttct ggcggtgctg atcgatgcgc tgttcgattt
    11581 actaatcgca ctgttgaagg tgaaacgtaa tgattgaatt tagccatgtc agcaaactgt
    11641 tcggcgcaca aaaagccgtt aacgatctca atctcaattt tcaggaaggg agtttttcgg
    11701 tgctgattgg cacatctggc tccggcaaat ccaccaccct gaaaatgatt aaccgcctgg
    11761 tggaacatga cagcggagag atccgctttg ccggagaaga aattcgctcg ctgccagtac
    11821 tggagttgcg ccgccgtatg ggctatgcca ttcaatctat tggcctgttt ccccactgga
    11881 gcgtggcaca aaacattgcc accgtgccgc aattacaaaa atggtcgcgg gcgaggattg
    11941 acgatcgtat cgacgaatta atggcgctac tggggctgga gtcaaatttg cgtgagcgtt
    12001 atccacatca gctttccggt ggtcagcagc aacgtgtggg agtagcgcgc gcactggctg
    12061 ccgatccgca agtcttactg atggatgaac cttttggcgc actggacccg gtaacgcgcg
    12121 gcgcgttgca acaagagatg acgcgcattc accgtttgct ggggcgtacc attgtgctgg
    12181 tcactcatga tattgatgag gcgctacggc tggcagaaca tctggtattg atggatcacg
    12241 gtgaagtggt gcagcagggc aatccgctga cgatgctgac tcgtccggcg aatgattttg
    12301 tccgccagtt ttttggacgt agtgaactgg gtgtgcgcct gctttcgtta cgtagtgtgg
    12361 cggattacgt gcgtcgcgaa gaacgggcag aaggtgaggc actggcagaa gagatgacgc
    12421 tgcgcgatgc gctctccctg tttgtcgcgc ggggatgcga ggtgctgccg gtggtgaaca
    12481 cgcagggcca gcctagcggc acgctgcatt ttcaggatct gctggtggag gcgtaagcgt
    12541 atgaagatgt tgcgcgatcc gctgttctgg ctcattgctt tgtttgtggc actgattttc
    12601 tggctgcctt acagccagcc gctgtttgct gccttgttcc cacaactgcc acgacccgtt
    12661 tatcagcaag aaagttttgc agctctggca ctggctcatt tctggctggt gggaatttcg
    12721 agtctgtttg cggtgatcat tggtactggt gccggaattg cagtcactcg cccgtggggc
    12781 gcggaatttc gcccactggt agaaactatt gccgccgttg ggcagacttt tccgcctgtc
    12841 gcagtgctgg cgattgctgt tccggtgata ggctttggtc tgcaaccagc gattatcgcc
    12901 ttgatccttt atggtgtgct gcccgtcctg caggcgacac ttgccgggct gggagcgatt
    12961 gatgccagcg tgacagaagt tgcgaaaggt atgggaatga gtcgaggtca gcgactgcgt
    13021 aaggtcgagc taccgctggc ggctccggtg attctggcgg gcgtgcgaac ttcggtgatt
    13081 atcaacattg gtacggcgac gatcgcctca acggtagggg ccagcacgct gggtacgccc
    13141 atcatcatcg ggcttagcgg atttaatacc gcgtatgtga tccagggggc gttactggtg
    13201 gcactggcgg cgatcatcgc agaccgcctg tttgaaaggc tggtgcaggc gcttagccag
    13261 cacgcaaaat aaaggtataa cctgcgagca tgacgccacc aattccgcct aacgccataa
    13321 acaggaacag ggcgatgacc ccaattttag ctatgcgcat aatgcactcc ttatgttaac
    13381 gaaaggattg tacagtaaag cgcatttgtt aacgaatcat taaatgccga gtgggaaaat
    13441 atcatagcct tgttcttgcc aactggtgag ttgctgttgt tgggcggagg ttcgattttc
    13501 accgcaccac accagcaatg tacggccttc gaacagttca gggcgtagtt gattgagcga
    13561 gtgggcgagg acatcaatgc gccatccttg ttgactggca atccagccct ccagccacag
    13621 acgggtggta tcgtgaatat tccagccaac caccagcgca tctttaccct gttttttacg
    13681 tgccgaagcc agacagatgg cgatgtagtt gatcagtacg ccgtcgagga tcgccagcag
    13741 cgcctggaga gtcggttgtt ggcactgaag ccgtcggcgc agaggaataa acaaatgcgt
    13801 ggtgagcgtc tgggcggggt aatcctgacc gcgctctttg atccacgttc gcaggctatg
    13861 cagattgccg ctttgcaggt aggtcagtaa tgtttcttgc tgatcgcgcc agccgttctg
    13921 cacatcaaca ttttcattac tgagcagcat tttaactttg ctgacctgca cgccgttgtc
    13981 gatccagcgt ttgatctcgc ggatacggtc aatatcggca tcgttgaaca gccgatgacc
    14041 gccgtctgtc cgttgcggtt tcagcaatcc gtaacgcctc tgccacgcgc gtaacgtgac
    14101 agggttaata tcacaaagca acgccacttc accaattgtg taaagcgcca tcgtctcacc
    14161 cttgctcgcg aggtcccggt ttaactttag acgcagtttt gcgaaccagg tagttttgcc
    14221 cgttttttgt gcatttatag ggtgatttta tttttgccag gcgattttga gtgatcgtac
    14281 tcacgaattc tcatttttct gcaagagttc aaagaaagtt aaacgcaggc aatgtatgtt
    14341 acgcgtttta aagggaagtg tggtttgcgg gtatgtacga ttttaatctg gtgttgctgc
    14401 tgcttcagca gatgtgcgtt tttttagtca ttgcgtggtt aatgagtaaa acgccattat
    14461 tcataccgtt aatgcaggtc acggttcgtc tgccgcataa atttctctgc tacatcgtct
    14521 tttccatctt ctgcatcatg ggcacctggt ttgggttgca cattgacgat tctattgcca
    14581 atacccgtgc gataggcgcg gtaatgggcg gcttactcgg cggtccggtc gtcggtgggc
    14641 tggttgggct gaccggtggc ttacatcgat attcgatggg gggcatgacc gcgttaagtt
    14701 gcatgatctc gaccatcgtt gaaggattac tcggtggcct ggtacacagc atcctgatcc
    14761 gccgcgggcg cactgataaa gtctttaacc ccattaccgc cggtgccgtc acgttcgtcg
    14821 ctgaaatggt gcaaatgctg atcatccttg cgatcgcccg accttatgaa gatgcggtgc
    14881 gtctggtgag taatattgct gcgccaatga tggtcaccaa taccgtcggc gcggcgctgt
    14941 ttatgcgtat attgctcgat aaacgcgcga tgtttgaaaa atacacttcg gctttttctg
    15001 ccactgcgct gaaagtggcg gcctcgacgg aaggcatttt gcgccagggg tttaacgaag
    15061 tgaacagcat gaaagtggca caggtgctgt atcaggagct ggatattggt gcagtcgcga
    15121 ttaccgatcg agagaaattg ctggccttta ccggaattgg tgacgaccac catttacccg
    15181 gcaaaccgat ttcttcgact tacaccttaa aagcgattga aaccggtgaa gtggtctacg
    15241 ctgatggcaa cgaagtacct tatcgttgct ctttgcatcc gcaatgcaaa ctggggtcga
    15301 cgctggtaat tccgttgcgt ggtgaaaatc agcgggtgat gggcaccatc aaattgtatg
    15361 aagccaaaaa ccgtttattt agttcaatca accgcacgtt gggtgagggg attgcgcaat
    15421 tgctttcggc gcagatcctt gccgggcaat atgagcggca aaaagcgatg ctcacccagt
    15481 cagagatcaa actgcttcac gcccaggtga atccccattt tttgtttaat gcgcttaaca
    15541 ccattaaagc ggtgatccgc cgcgacagcg aacaggccag ccagctggtg cagtatcttt
    15601 ccactttttt ccgcaaaaac ttaaagcggc cttcggagtt tgttactctc gccgacgaaa
    15661 ttgaacatgt gaatgcttat ctgcaaattg aaaaggcgcg cttccagtcg cggttgcagg
    15721 tcaacattgc tattccgcaa gaattatccc agcagcaatt gcccgcgttt accctgcaac
    15781 cgatagtgga aaacgccatt aaacatggga catcacaact gctggataca gggcgagtgg
    15841 caatcagcgc ccgacgtgag gggcaacatt taatgctgga gatcgaagac aatgccggtt
    15901 tgtatcaacc ggtaaccaat gccagtgggc tggggatgaa tctggtggat aagcgtttac
    15961 gtgaacggtt tggcgatgac tatgggataa gcgtcgcctg tgagcctgat agttacaccc
    16021 gaataacgtt acgactacca tggagggacg aggcatgatt aaagtcttaa ttgtcgatga
    16081 tgaaccgtta gcacgggaga acctgcgcgt atttttgcag gagcagagcg atattgaaat
    16141 cgttggtgag tgttcaaacg ccgtggaagg gatcggcgcg gtgcataaac tgcgcccgga
    16201 tgtgctgttt ctcgatatcc agatgccgcg catcagtggt ctggaaatgg tggggatgct
    16261 cgacccggaa catcgtccgt atattgtttt tctcaccgcg tttgacgaat acgctattaa
    16321 agcctttgaa gaacatgcct ttgactatct gctgaagcca attgatgaag cgagactgga
    16381 gaaaacgctg gcgcgtttgc gtcaggaacg cagcaagcag gatgtttcgc tgttaccgga
    16441 aaatcaacag gcgctgaaat ttatcccttg tacggggcat agtcggattt atttgctgca
    16501 aatgaaagat gtggcatttg tcagcagtcg gatgagcggt gtctacgtta ccagccacga
    16561 agggaaagag ggctttaccg aattgacatt acgtaccctg gaaagtcgta caccactact
    16621 gcgctgccat cgtcagtatc tggttaacct cgcgcattta caggagattc gtctggaaga
    16681 taacggccag gccgagttga ttttgcgtaa tggcttaacc gtgccggtca gccgccgtta
    16741 tctgaaaagc ttaaaagagg cgattggcct gtaaaagact gctaaaatgg ctttttgcct
    16801 catcaacacc tgaaggcctc atgctaagta acgatattct gcgcagcgtg cgctacattt
    16861 tgaaagccaa taataatgac ctggtgcgta ttctggcgct gggtaatgtc gaagccaccg
    16921 cggaacagat cgccgtctgg ctacgtaaag aagacgaaga gggttttcag cgttgtccgg
    16981 acattgtttt gtcgtcattc ctcaatggcc tgatttatga aaaacgcggc aaggatgagt
    17041 ctgctccggc actggagccg gaacgtcgca ttaataacaa catcgtgctg aaaaaattac
    17101 gcatcgcgtt ttcgctgaaa accgatgaca ttctggctat cctcaccgaa cagcagttcc
    17161 gcgtttcgat gccggaaatt acggcgatga tgcgtgcacc ggatcataaa aacttccgcg
    17221 aatgcggcga tcaattttta cgttattttc tgcgtggact ggcagcacgc cagcatgtga
    17281 agaaaagcta agacgggtat ggcggccatg cgaaacatgg ccgccgacag attatttcac
    17341 ttctttaaaa ccagcggctt tcatcaccag ttccatttgc gccatagtga tacctttttt
    17401 ggcatcttca gcagaaacgt tgattcctga aataccctgc agggctttaa aatccacttt
    17461 ttccatatcg atagtcacgt tttcctgcgc gtaggtatcg gtataggtta atttttcttc
    17521 aacacccgcg atgtttttgt atttggcgct taacggctca agtgtcttgg cagcgtcttc
    17581 tttggttgtt gcaccaatag aggcaaattg aattttggtt tctgaagatt gcttaagcac
    17641 cttgtcacct ttgtagacat aggtaatggc aatttcagtg ccgttcagat tggcgctgaa
    17701 tttcttcgat tcttctttgt caccgcagcc agcaagagag aaaaccagaa cagatgcaac
    17761 aacaagggaa aacagcttat tgaaagcctt catgtaaaac tccattttat ttaatcaagg
    17821 aactggtgac tctcaccagg ggctatatag gatatgccta ataccgtggc gtgagcagtc
    17881 cggaactgga gtagaactct tagtaaaaag cactatttca tccttgttgc tgaagcatgg
    17941 ggaataattg ttcgcacagc aaaacaccgt tattcattgc ttctacccgt gcctcgcttt
    18001 ctgtattacg aaattgtccc aatacatgtg ccagccgata aaaacccacc gcggtgaggt
    18061 cattcgccag caactctgcc tgaccaatag cactctgttc ctgatagcgc cagccgttat
    18121 ggagcagttg aataagtaat gcctggcagc gcatcagcaa ctgatgagca gtagacggca
    18181 caggcagaac gctggcagaa ggtagtgatg ccaccacagg cgcagtttct gcgtccagcg
    18241 cccaggcacg agtctttgtc atcattacct gtggttccag tgtcaattgc ccatcaacaa
    18301 aactgacaaa gccagaaacc agacacacgg ggtcgtctgt ttgttgcaaa agcgccgcca
    18361 tgcgttcaac ggcataaggc gcgctggctg aggctggcag tgataacgtc agctgattat
    18421 cttcaccttc gccgctgatt acctgcgcat ccagcgtttg gcggctgcta tcccaaccga
    18481 gtgaaataca ctcagcgacc ggcagaataa ataagttatc gacctgatta agaggccgta
    18541 tgcaggcggg gggacgctgg cgtaaatatt cccgcaaagc cacaatgccc ggctggcgca
    18601 acggcgcgct caacatttgc caggcatctg gcgacagtgg aacaacacta ctcaagcggt
    18661 tacgggtcgc gaacagcagt tcgccttcgg cactgcgacg cgctgcttgt gaaacaattt
    18721 gcccacctgc cagtgcgcca gcctgaaaac taaatagccg acgcgtcgct gccggtgagt
    18781 tttcctgttc acttcgcggc caactgcgcg aaaggtgcaa aatactgccg gtgtcgggat
    18841 cggtaaacca gatgcgtaaa ccataatgct caatatcctg ccagcaacgc atacctaaag
    18901 acaccagccg cagatgatca agctttgctt ctccggcaat gcctgcgcca acgaccgtgc
    18961 gccacggcac aggaggaact tcaccaacac tgtcgcgccg ggccatctct tgtgcgcaat
    19021 ttaatcgact gtttaatgcc gcaagctgac gtaagcattc tccggcatga tagtggctgg
    19081 cgcgggcgtg gaaggcatca acgcttgccc gtagctgtcg tagtgattca ctcacccatc
    19141 gccagttgca gcgttccgcc gcctgctgcg cgcgactaaa agcggcctcg tagtgaataa
    19201 gcggctggct gatgccgccc agccataatg cctggcttaa ttgctgaaca tattgacgac
    19261 acgcgttacc ctcgtcgttg gcaaacggat cgttagatga tgtgacgtgt tcgctgcgca
    19321 tctgccagat taaatgagtc aattccgctt gctgcgcttt ggcctggaca aaggcctgta
    19381 ctgccagtac gacatgttcg caaagtgtgc cttcaataca atcacaacgg gcgaaacgaa
    19441 tgctgctacg ggaataaaaa cgcacatcgc tcatcggtaa gcgggcagag ggaatttcgc
    19501 ccggcgtaca gaacaactca atggtgatgc ctttagcgac cagcgcctgt gcgcgtttgc
    19561 gggtagcatc gggaagggta gccagttctt ccagccagat tgccggatcc cactcttctt
    19621 ctttttccgt agactgagtg gtggcacaaa gtcgttgata acttaacacc agcatcacgc
    19681 gatgacggca cataccgttg gccccgcaac tgcactgagc ctctttcagt gcctggccgt
    19741 tcgccagctg ggtacggaca ccgtcactga aggtggcgat taaagcgtcg ttctcatggc
    19801 tgatctccgg gacgttgcca ttttccagtt ccttaagact gcgcttaaca aaaccggcat
    19861 tgcttaacgc cgtcagggcc tgcggtgtca gttctaataa ttccggacgt agtgaattca
    19921 tgactgaaga ttctccgcaa gccatgatgc cagctcgccc ggcgtcatgg cggctatttg
    19981 tgcgccgaca ttaaccagcg cctgggccgt atcgcggtca tagcaaggtg ttgcggtgct
    20041 atcgagcgct gccagtccca gcactttgat gccgctctgg acacactttt tcacctgatg
    20101 cgtcagtaat gatgatgaac ccccttcgta aaaatcgctc acgaggataa tgacgctttt
    20161 cgctggttgt tcaataagtt gccgaccata ctccacggca ctggcgatat tggtcccgcc
    20221 gcccaactgt actttcatta ataactctac cggatcggca acgtctgccg tgagatcaac
    20281 gacgcttgtg tcaaacgcca ccagatgggt acgaatgccg ggtaactgcc acaaacaggc
    20341 cgccatcacc gcagagtgga tcaccgaatc gaccatcgat ccgctttgat caaccagtaa
    20401 gaccagttgc cattgttcgc tttggcgttt aatgcggctg ttaaagcggg gggattcgat
    20461 atacaacttg ccgtgttgcg ggtgccagtg ttgcaggttg gcgcgcagag tacttttgaa
    20521 atcaaagttt cgcgccagtg gaataaatga acggcgacgg cgatcgcgga caccagaaaa
    20581 agcctggcga acttcctttg ccagtcgcgc cataatttct tcaacaacct ggcgcactat
    20641 ctggcgggcg gcagccagta cttcgggatt catcagatgt ttggtgtgca aaacggcgcg
    20701 tagcaggctt tcggaaggct gcatacgttc cagcacgtcg agatttgtca ccacatcttc
    20761 aatgccgtag cgcagtacgg catcgctttc cagccgctca atcacctgtt gcggaaacag
    20821 cgtgtgaata ctgttgatcc actcaggggt ggtgagattt gagccaccta atccaccgga
    20881 gcgttcacca cgctggagcc gttcaggatc gcgcccatac agccactcca gcgcgtggtc
    20941 tatctgccgg gcgttgtcat ccagcccaca aagcgtcgtt tctgccgctt cgccaagaat
    21001 taatcgccag cgttgtagct cacgggtggt cagaagatcg ttcagttcag acatggaaaa
    21061 ctccccagtg ttgcagcgat gccagtgcct gctgttcgag ttgttgatga tgtgcaatgg
    21121 cttgcggtgg acaatgcaac ggcatttgca gtgccgaaac gggaagttgc gccagttgat
    21181 aatgctcaag cacctgatgc gccagcgtcc cgcgttctcg tggcggtagc caggccattg
    21241 ccgcgcgtaa atcgggcagg gcattggtaa aatcggcatc acttagttga tttaaatgac
    21301 tgttgaaacc ggcgataaat gccggctgac aggccagttg atgacgggct aatgccagca
    21361 aaccgtgcag cgcctcgccg gattgtgccg gggataactg cgccagcatc gtcagcgccg
    21421 cttcggcact ggcgttggga tgttccagac gcattagtgc gccaagaacc gcgccgcgat
    21481 ccagcgccgg ggcatgattt gcctgactgt ggcgctccag taaggctacc gccgcagaaa
    21541 gcgaaacgcc ggataaatta acgccgctat gtagatcgcg cagaatatgg caaagcgctt
    21601 gccagctatg caggtgagcg tgaaactcct tttcatctgg tctgccgtta gattcacaca
    21661 gccacagcgt gcgatcgata gccgcgcata acgtcgtttg taatatctgc gcgccttgca
    21721 taccgctaat ttcatccagc cgccataagg catatagcac ttccagcgcc tggcccattt
    21781 cggcaaattg attttcctgg gcgattaata atgtgagttg ctccagtaat tgttgactga
    21841 aggacgccag acccgctaac gccgcctggc taagacatgt ggtgatactg ccgattccac
    21901 ccgcgtccag catatcggct tctaatttat ggcgtgcggc ttcctggagt gtggcaccaa
    21961 agcagacggc ctcaatcaat gccgcatgct ggctaagcgg gcgggttaat ttccagcgtt
    22021 cttcactgtt gcctgcaagt gtcagtgtgc ttccctgctg gcgtacaatc cctgggatct
    22081 ccagtattgc cagccgatgt aacacctgac tttgcgctag tccattgggg ttaaagcgat
    22141 ttaaggtaag ctccgccgga agagagattg cggtacgttc cagttcgcag gtgacatctt
    22201 ttggcagagg cggttgtggc gtagaagggg caagtttacc gaatccgtca cccgctaatg
    22261 tgtctatcaa cgttagcaga atcggatcgg tgtcgggatg aataacgccg cgatagctcc
    22321 acggcaacgg cgcattcagg gcttctttaa tcagcgagcc tgctatggca tccagccagt
    22381 cagtgcgtaa cggtagttta tgtccgcgca actgtgccag cgccatcgaa tgcagatgag
    22441 cggcagccat atccgcggtc gaagcaggca agtggtgttg gcgcaaacgg gtaagaaccg
    22501 tttttagcag ttgttctccc gcctgctgta agccccactg ccagcaccag ctttgccaga
    22561 ccggggcagg cattcctgaa aggtatcctg ccagcacatc aaggcgcttt tcactgtagg
    22621 gtgtgagata acaacctgta acggcatctg ccagcgaggg caattctggc gtgttaatgt
    22681 cctgcgggca ttcgcgccac atttttgcca gtgccggagc gtgccagcca ccgcagacga
    22741 ctaacacgtc gccattattt tgctgcaccg cccatgcaat ccagcgagcc ataaaggctt
    22801 cgcgctgacg attgagtgca tcgccgggga aatcgccccg cagttgggca aaatagtgcg
    22861 ccagtgcaga gggtaatgca gtttgctggc tttcatcttc gaacaagtga tcccacaggg
    22921 tatcgctgtt atccatgcgg gtggcacgca gcagcaaggt ctggctttcc tcttgcgtat
    22981 caggcgaatc gtcctcttct tcactctgtg cccagcaagg caaatcgatg aagtaagttt
    23041 gtgcctgaat acgacgagcg gcttgtagcg cctgccactc cggcgaaaat tcagcaaatg
    23101 gcgtccaggc accacgcccg ggggctgcac cgtcctgata ctggcaataa ctgtaaatag
    23161 ctaccggaag ctggtgggct aaaaacagtt cgtctacccg gtcattaaaa tcagccgggc
    23221 cttcaatcaa cacgtatcgt ggccgctggc tttcgattaa cgatttcacc agccgggcgc
    23281 aggccggact atgatgccgg atgccgacaa ttaacggctc gctcatgcat ctttcctcac
    23341 ggcaggcggt ggcgggcttg ataataagcc tgccagtgag cttctttatg tgtggcgaca
    23401 cgctgttcga agtaacggcg cagacgcgcg cgatcttctt cgttatcttt gacgatggtt
    23461 ccggcaatac agtccaccag gtcggctggc tcacctgcgc gattcgctaa aaaccaggcg
    23521 cggacgccca cagcgtgagc cacattcacg gcttcagcgg tggacataat tgccgtcagt
    23581 gtatccattg acgttttctt ttcgccgttg gcacgcagat cgcggaaggt gcggaccagt
    23641 aactccagta ccgcgtcagg cactttatgc ggaataccgc tatgggccag caaacgcgcc
    23701 gaagcactgg cgacaagctc cagctcctgg gcgaaatcca taatcggaaa gacagtttca
    23761 aaatcgaagc gacgctttag cgcagcactc atttcattga cgccgcgatc gcgggtattg
    23821 gcagtggcaa taatattaaa gccttctcgc gcatagagct gactggcttc accagtgagt
    23881 tccggcaccg tcatcaccct gtcagaaagc atacctaaca gacagtcttg cacttccagc
    23941 ggtgtacgcg ttatctcttc aaaacggacg attttgccgt cgcgcattcc ctggtaaagc
    24001 ggggcaggga ccagcgcttc cgttgatggg ccatgattga taagcagagc gtagttccac
    24061 ccgtacttga tttgatcttc ggtagtagat gctccgccct ggatggttaa cccggcatcg
    24121 ccgctaatag cggttgccag taattcagac aggagagatt ttgcggtgcc aggttccccc
    24181 accaacatca aaccgcgccc ggtagcaaga gtgactaaca tacgttcgac agcggaaggg
    24241 ctaatcacca ctttacggct gatattttgc gcgttatcgc caaggataaa agcacgggcc
    24301 gcaggcaaac ttaactgcca gccgggcggg caaggtgcgt tatcattttg ttttaatttt
    24361 gccagttcat cggcgtgtaa cacagcagca ggcggacgct gcagatgatt attctgcggt
    24421 gacatatatt catccctgaa atgtattaat gaaataaacg atattttacg cgcaataaat
    24481 gtttggcgca ttagttgttt gagcgaaaaa tatctggaaa gcagatcaca gtaaataaaa
    24541 aaacctgctg gtgaggcagg ttcttttatt tactattttc aggcctaaca ccacagcctg
    24601 tgcaacatcg tgttccaacg aacgaattat aaccacggct ggaccccgtt gcaattaaga
    24661 taatgagatt tataatccag accgttggga cgttgcgccg gatcgaacct atttcaagtc
    24721 ctggctaccg gacgggtggt gctgcaggtg gtgccctgat ccggagtgca acatcgtggt
    24781 ccaacatacg taaaccagta tcgacaggca gtgtaagggt aaggtgctgg tgttgcgctc
    24841 cttaataagg agagcggatg gactcgctga cacaa
//
LOCUS       DAPGOF010000064        24756 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.064, whole genome shotgun sequence.
ACCESSION   DAPGOF010000064 DAPGOF010000000
VERSION     DAPGOF010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.064"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..112
                     /locus_tag="RHV13_002241"
     CDS             <1..112
                     /locus_tag="RHV13_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP pyrophosphate phosphatase"
                     /protein_id="HDV1241771.1"
                     /translation="DEVIIINKITPKKIATEEIATKQSTAEEIIANTIIE"
     gene            complement(130..317)
                     /locus_tag="RHV13_002242"
                     /pseudo
     CDS             complement(130..317)
                     /locus_tag="RHV13_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(530..1189)
                     /gene="rluA"
                     /locus_tag="RHV13_002243"
     CDS             complement(530..1189)
                     /gene="rluA"
                     /locus_tag="RHV13_002243"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HDV1241772.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(1201..4107)
                     /gene="rapA"
                     /locus_tag="RHV13_002244"
     CDS             complement(1201..4107)
                     /gene="rapA"
                     /locus_tag="RHV13_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HDV1241773.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            complement(4271..6622)
                     /gene="polB"
                     /locus_tag="RHV13_002245"
     CDS             complement(4271..6622)
                     /gene="polB"
                     /locus_tag="RHV13_002245"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HDV1241774.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAVKIG
                     RALVQHVNAWWAETLQKQQLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            complement(6697..7392)
                     /gene="araD"
                     /locus_tag="RHV13_002246"
     CDS             complement(6697..7392)
                     /gene="araD"
                     /locus_tag="RHV13_002246"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDV1241775.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPAIGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            complement(7561..9063)
                     /gene="araA"
                     /locus_tag="RHV13_002247"
     CDS             complement(7561..9063)
                     /gene="araA"
                     /locus_tag="RHV13_002247"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HDV1241776.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            complement(9074..10774)
                     /gene="araB"
                     /locus_tag="RHV13_002248"
     CDS             complement(9074..10774)
                     /gene="araB"
                     /locus_tag="RHV13_002248"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HDV1241777.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRADVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDTAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            11113..11991
                     /gene="araC"
                     /locus_tag="RHV13_002249"
     CDS             11113..11991
                     /gene="araC"
                     /locus_tag="RHV13_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HDV1241778.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            12077..12841
                     /locus_tag="RHV13_002250"
     CDS             12077..12841
                     /locus_tag="RHV13_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDV1241779.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLVTAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(12928..13626)
                     /gene="thiQ"
                     /locus_tag="RHV13_002251"
     CDS             complement(12928..13626)
                     /gene="thiQ"
                     /locus_tag="RHV13_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HDV1241780.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASALLGITG"
     gene            complement(13610..15220)
                     /gene="thiP"
                     /locus_tag="RHV13_002252"
     CDS             complement(13610..15220)
                     /gene="thiP"
                     /locus_tag="RHV13_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HDV1241781.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGDWS
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVTALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            complement(15196..16179)
                     /gene="thiB"
                     /locus_tag="RHV13_002253"
     CDS             complement(15196..16179)
                     /gene="thiB"
                     /locus_tag="RHV13_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HDV1241782.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPVVKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEKLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     regulatory      complement(16210..16309)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            16551..17120
                     /locus_tag="RHV13_002254"
     CDS             16551..17120
                     /locus_tag="RHV13_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HDV1241783.1"
                     /translation="MKVRNPEQISISASNTTKDPGLTNSQIIRMVNLVQKTKNMNIFE
                     ELWETLRNLFRSDKHSQTAARQILKDAFYFQNSDDYSKYFTGAVDGKARDQLTHWLIK
                     FNELKEHARDPENMAAKASLSPKDTLCVSFFIGDEAIFTLKLQLKENTRTGCIDLSNA
                     YFNGVVICGIDCLEVDMSNAETNNSRWYD"
     gene            complement(17349..19007)
                     /gene="sgrR"
                     /locus_tag="RHV13_002255"
     CDS             complement(17349..19007)
                     /gene="sgrR"
                     /locus_tag="RHV13_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HDV1241784.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDPE"
     gene            19096..19227
                     /gene="sgrT"
                     /locus_tag="RHV13_002256"
     CDS             19096..19227
                     /gene="sgrT"
                     /locus_tag="RHV13_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HDV1241785.1"
                     /translation="MRQFYQHYFTETAKLCWLRWLSVPQRLTMLEELMQWEGSHSDY"
     gene            complement(19335..19940)
                     /gene="leuD"
                     /locus_tag="RHV13_002257"
     CDS             complement(19335..19940)
                     /gene="leuD"
                     /locus_tag="RHV13_002257"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HDV1241786.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMR"
     gene            complement(19951..21351)
                     /gene="leuC"
                     /locus_tag="RHV13_002258"
     CDS             complement(19951..21351)
                     /gene="leuC"
                     /locus_tag="RHV13_002258"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HDV1241787.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKTAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMVIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            complement(21354..22445)
                     /gene="leuB"
                     /locus_tag="RHV13_002259"
     CDS             complement(21354..22445)
                     /gene="leuB"
                     /locus_tag="RHV13_002259"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HDV1241788.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     IELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            complement(22445..24016)
                     /gene="leuA"
                     /locus_tag="RHV13_002260"
     CDS             complement(22445..24016)
                     /gene="leuA"
                     /locus_tag="RHV13_002260"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDV1241789.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            complement(24109..24195)
                     /gene="leuL"
                     /locus_tag="RHV13_002261"
     CDS             complement(24109..24195)
                     /gene="leuL"
                     /locus_tag="RHV13_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HDV1241790.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
BASE COUNT         5629 a         6618 c         6624 g         5885 t
ORIGIN      
        1 tgatgaggta attattatta ataaaatcac tcccaaaaaa attgctaccg aagaaattgc
       61 tactaaacaa agtactgctg aagaaattat tgccaacaca attattgaat gatccagaat
      121 aagctaaggt tgaaggggct ggaacgcccc ttcaacctta gcagtagcgt aggatgattt
      181 cacaattaga aagacctgca tgatgagcga gagaagaggc tcgtgacgca aggcgtcgtg
      241 cacgacccgg atcaccgaga tgggcatcgc caaccagact gctaattagc ccatgaataa
      301 caatcagaaa ggaccataac agacccgata aaatgaaata gtagagacga tcaacgggtg
      361 aagaaaaagt tcaaaaaatc gctgtggagc agaaagggaa ttaccgaatg gaaagcgtag
      421 ccacacgcaa caactgaaag cagtttggca gaaacaaaaa atccccggac tcggggattt
      481 atgtacaaga ggcagccctt aggatgaggg tataaacgta caggaaaggt taaaaatccg
      541 ctggcgcttt aaacgtcatg ctattgccat acgccggatg ggtaatcgtc aacatctctg
      601 catgtagcaa caaacgtggt gccatcgctc tcgcttccgg tgatgcataa aaacgatcgc
      661 cgagaatcgg atgacccagc gccagcatat gcacacgcaa ttgatgcgaa cgcccggtaa
      721 tcggttttaa caccactctt gccgtgttat ccgccgcata ctccaccact tcatattccg
      781 tctgcgcagg tttacccgtt tcgtaacaga ctttctgttt cgggcggttt ggccagtcgc
      841 aaatcagtgg cagatctacc agaccttccg caggggatgg atgcccccag acgcgggcca
      901 catactgctt tttcggctcg cgctcgcgga actggcgttt taactcccgc tccgcggctt
      961 tggtcagcgc cactacaatc acgccgctgg tagccatatc cagacgatgc accgattctg
     1021 cctgcggata atcacgctga atgcgcgtca tcacgctgtc tttgtgctct tccagacgac
     1081 ccggcactga caacaaaccg ctcggcttgt tgaccaccat aatatggtca tcctgataca
     1141 ggataaccaa ccagggttcc tgcggtggat tgtagttttc catccccatt ttcggctccg
     1201 ttactgatgc gttacaacga tcaaacgcag ggcatccaga cgccaacctg cctgatccag
     1261 gctttccatt acctgctgac ggttgctctc aatggcggtc agttcgtcgt cacgaatgtt
     1321 cgggttcact gcgcgcagcg cttccagacg agacagctcg gcagacagtt tttcgtcggc
     1381 ttcgttacgt gcggcatcaa tcagtgcacg ggcagatttc tcgatctgtg cttcacccaa
     1441 ctgaaggata gcgtgaacat cctgctgcac ggcgttaacc agtttgctgc cggtgtgacg
     1501 gttaaccgcg ttaagctggc ggttaaaggt ttcaaactcc acctgggccg ccaggttgtt
     1561 gccgttttta tccagcagca tacgtaccgg cgtcggtggc aggaagcggt tgagctgcaa
     1621 ctgcttcggc gcctgggctt caaccacata aatcagttcc accaacagcg tacctaccgg
     1681 caacgctttg ttttttaaca gtgaaatcgt gctgctaccg gtatcgccag aaaggatcag
     1741 atccagaccg ttgcggatca gcggatgctc ccaggtaata aactgtgcat cttcacgcgc
     1801 cagcgccact tcacgatcaa aggtgatggt gatgccatct tccgacaggc cagggaagtc
     1861 cggcaccagc atatgatcgg acggcgtcag cacgatcatg ttgtcgccgc gatcgtcctg
     1921 attgataccg ataatatcga acaggttcat ggcgaaggcg atcaggttgg tatcgtcatc
     1981 ctgctcttca atgctttctg ccagtgcctg ggctttttcg ccaccgttgg agtggatttc
     2041 cagcaggcgg tcacgaccct gttccagctg tgctttcagc gcttcatgtt gctcgcggca
     2101 gtttttgatc agatcgtcaa agccttcggt ttgatccggt gaagccagat agttaatcag
     2161 atcgttgtat acgctatcgt aaatagtgcg tccggtcggg caggtgtgct caaatgcatc
     2221 cagaccttcg tgataccagc gcaccagcac cgactgagcg gttttctcca gataaggcac
     2281 atggatctga atatcgtgcg cctggccgat acgatccaga cgaccaatac gctgctccag
     2341 tagatccggg ttgaatggca ggtcaaacat caccatgtgg ctggcgaact ggaagttacg
     2401 tccttcagaa ccgatttctg agcacagcag tacctgtgcg ccggtgtctt cttcggcaaa
     2461 ccaggcggca gcgcggtcac gttcgataat cgacatacct tcgtggaaca ccgcagcgcg
     2521 aataccttca cgttcgcgca gtacctgctc cagttgcagc gcagtggcag ctttggcgca
     2581 gatcaccagc actttctgag agcgatggct ggtcaggtag cccatcagcc actcaacgcg
     2641 cggatcgaag ttccaccagg tggcgttatc accttcaaat tcctgataaa tacgctccgg
     2701 gtagagcata tcgcgagcac gatcttccgc acttttacgt gcgcccataa tgccggagac
     2761 tttaatagcc gtctgatact gcgtcggtag cggcagctta atggtgtgca gctcgcgttt
     2821 cgggaatcct ttcacaccgt tacgcgtgtt acggaacagc acgcggctgg tgccgtggcg
     2881 atccatcagc atcgaaacca gctcctgacg ggcgctctgg gcatcttcgc tgtcgctgtt
     2941 tgctgcttgc aacagcggct cgatatcctg ctcgccgatc atctcgccaa gcatgttcag
     3001 ttcgtcattg ctcagtttgt tacctgccag cagcatggca acggcgtccg caaccggacg
     3061 ataatttttc tgctcttcaa cgaactgcgc aaaatcgtgg aaacggttcg ggtccagcag
     3121 acgcagacgg gcgaagtggc tttccatccc cagctgttcc ggggtcgcgg tcagcagcag
     3181 aacgcccggc acgtgctctg ccagttgttc aatggcctga tattcacggc ttggcgcatc
     3241 ttcgctccac accaggtgat gcgcttcatc gaccaccagc aggtcccatt cggcttcaca
     3301 gagatgttcc aggcgctgtt tgctacgacg ggcaaaatcc agcgagcaaa tcaccagctg
     3361 ttcggtgtcg aacgggttgt aagcatcgtg ctgagcttcg gcataacgct catcatcaaa
     3421 tagcgcaaag cgcaggttga aacggcgcag catttctacc agccactgat gctgtaaggt
     3481 ttccgggacg ataattagca cacgttcagc agcgccagag agcagttgct gatgcaggat
     3541 catcccggct tcaatggttt tccctaaacc cacttcgtca gccagcagga cgcgcggcgc
     3601 gtggcggcga ccaacatcat gagcgatgtt gagctgatgc gggatcaggc tggtacgctg
     3661 accgcgcagg ccgctatacg gcatacggaa ctgttcgctg gaatacttac gcgcgcgata
     3721 acgcagcgca aagcggtcca tacggtcaat ctgcccggca aacagacggt cctgcggttt
     3781 gctgaacacc agtttgctat caaggaaaac ttcacgcagg gctacgccgg actcttcagt
     3841 atccaggcga gtaccgatat aggtcagcaa gccattttct tcttttactt cttcgacttg
     3901 catctgccag ccgtcatggc tggtaatggt atcaccaggg ttgaacatta cgcgggtcac
     3961 gggggaatca ctgcgtgcgt acagacggtt ttcaccagta gatgggaaaa gtaaagtgac
     4021 agttcgcgca tccaccgcga caacggttcc aagtcccaat tcgctttctg tatcgctgat
     4081 ccagcgttga ccaagtgtaa aaggcatatg tgttcggctc tatatcttta attgcaggca
     4141 ataaccaccc gctaccgtgc ttatgaggta gtggtgttat tcaggtccag gaatggaaag
     4201 ggcgctatgg tactggatgg caaagcattc gtcacgcatc aaaatggtat ctggcgaact
     4261 ctttttttgc tcaaaatagc ccaagttgcc cggtcataag tgtagcaaaa ttatcctcaa
     4321 taaaagggag tattccctcc gccacgggtt gtagctggcg ggtcagatag tgttcgtaat
     4381 ccagtggtga acgttggtag tccagcggct ccgggccgtt ggtggtccat acgtacttaa
     4441 tggtgccgcg gttctgatat tgcaaggggc gaccgcgctt ttggttttct tcatcggcaa
     4501 gacgagcggc gcgtacatga ggcggaacat tacgctgata ttcgctcagc ggacggcgaa
     4561 ggcgtttacg gtaaaccagc cgcgcatcca gttcacccgc catcagtttg tcgatggttt
     4621 cgcggacgta ctcctgatat ggctcgttgc ggaagatacg caggtaaagc tcctgctgaa
     4681 actgctgggc cagcggcgtc cagtccgtac gcacggtttc cagcccttta aacaccatcc
     4741 gctgtttgtc gccctcctga atcagcccgg cataacgctt tttactgccg gtatcggctc
     4801 cgcgaatggt tggcatcaga aaacggcaga aatgggtttc atactccagt tccagtgcgc
     4861 tggtcagctg ttgtttttgc agcgtttccg cccaccaggc gttaacgtgc tgcaccagtg
     4921 cacgaccgat tttcaccgct tcttcttccg aatgtgcgcc tttcagccag acaaaagtcg
     4981 agtcggtatc tccgtagatc acgtcgtagc cctgcgcttc aatcaacgct ttggtttgcc
     5041 gcatgatctg atgaccacgc atggtgatcg acgatgccag ccgcggatcg aagaagcggc
     5101 aggcggtggt gccgagcacg ccataaaagg cattcatgat gattttcagc gcctgcgaca
     5161 gcggtttgtt accctggcgt ttggcttcat cgcgcccgtg ccagatgtta gtcacaatct
     5221 ccggcaggca atgtttttct cgtgagaacc aggcatcgag aaaaccttcg gtactgtgct
     5281 ctggatcagg ctgcgccatg ccttccacca gcccgacggg atcaatcaga aaggtgcgga
     5341 tgatcgacgg gtacaggctt ttatagtcca gcaccagcac tgaatcataa agccctggcc
     5401 gtgaatccat cacgtagccg ccagggctgg cgtgcggtgg cacttcgccg agattaggcg
     5461 cgacataacc agcgcgatgc attcgcggaa aatagagatg accaaacgct gccaccgaac
     5521 cgccgtgccg atccaccggc aggccgttca ccgttgctcg ttcgagtaaa aatggcatga
     5581 tttcagtttt gtgaaagatc tgcgtcacca gctcgcaatc tttcaggtta taagttgcca
     5641 gcgcaggttt atcttcggcg aaacggcggt caatttcgtc cattcgatcc cacgggttat
     5701 cgatagattt tccttcgcct aatagctcct gagcgacagt ttccagcgag aatgaagaga
     5761 aattccagaa cgcggatttc agcgcctcga taccgtcgat aattagccga cctttagcct
     5821 gggcaaaaaa gacgccgttt ttaaagccgt gctcgcgcca ctccagctcg ctattatcgc
     5881 gcccaagacg cagcggaaga cggtaacgct cggcatgttt ttgcagcatt cgcagatcga
     5941 actgcaccac gttccagccg atgatcacat cgggatcgtg gtttgcaaac caggcgttga
     6001 gtttttccag cagcagcggg cggctggcga cgtattccag ttcgaaatca agcgaggagg
     6061 cgtcgccatt ctccggcccc agcatataaa cgatgcgctg cccgcagcct tccaggccga
     6121 tgcagtacag ctcaccgtgg cgggtggttt caatatctat agaaacccac ttgagcggcg
     6181 gacgatagtc gggatgcggt ttcagacggg cattaacgat agtgccattg tgcatatcac
     6241 cctcgaccca caccggtgag gtgataaacc gctccatcag atagcgttct ggcgggcgca
     6301 catcggcctc gtagacggta acgccacctt cacgcaggcg cttttcgtaa ttcatcaatt
     6361 ggcgatgggc gcgacagtaa aggccataca ccggctggcg gtgaaaatcc tttaacgcca
     6421 gcggtgtcag gcgaaagcct tgttcaccct gcaaaatatg ctgagcgcgg ggaacctgat
     6481 cggcgggaat aaacgccacg gactcttgcg gtgcaagcgt aacctgcaac ggcccgttgt
     6541 ccgtcgccag ccagaaggag acttctgtcc cttgcggggt gtcccgccag tgtcgggtta
     6601 agataaaacc tgcctgcgcc acgctgaaaa tccatcaaaa aaccaggctt gagtatagcc
     6661 tggtttcgtt tgattggctg tggttttata cagtcattac tgcccgtaat atgccttcgc
     6721 gccatgctta cgcagatagt gtttatccag cagcgtttgc tgcatatccg gtaactgcgg
     6781 cgctaactga cggcagaata tccccatata agcgacctct tccagcacga tggcgttatg
     6841 caccgcatct tcggcatttt tgccccatgc aaacgggccg tgggaatgga ccagaacgcc
     6901 gggcatttgc gctgcatcga taccctgttt ttcaaaggtt tctacgatga cgttaccggt
     6961 ttcccactca tattcgccgt tgatttctgc gtcggtcatt ttgcgggtgc agggaatggt
     7021 gccgtagaaa tagtcggcgt gggtggtgcc gattgctgga atcgactgac ccgactgcgc
     7081 ccagatggtg gcgtggcgcg agtgcgtatg cacaatgccg ccaatggagg ggaatgcctg
     7141 atagagcagc cggtgagttg gcgtgtcgga ggagggcttt ttcgtacctt caaccacttc
     7201 accggtttcg atgctaacca cgaccatatc gtcagcggtc atgacgctgt aatcgacgcc
     7261 ggaaggtttg atcacaaaga cgccgcgctc gcgatcaacg gcgctgacgt tgccccatgt
     7321 gagcgtgacc aggttgtgtt ttggcagcgc caggttggct tctaatacct ggcgtttgag
     7381 atcttctaac atgttgactc ctgagaggtc gtttcccctc accccggccc tctccccaaa
     7441 ggggcgaggg ggaaaacaat gccggatgcg ctttgcttat ccggcctaca aaatcgcagc
     7501 gtgtaggcca gataagacgc gtcagcgtcg catcaggcgt tacatacctg atgtggctac
     7561 ttagcgacga aatccgtaat acacttcgtt ccagcgcagt gcgtctttaa acgctggcag
     7621 gcgggtgtcg ttatcaatca ccgtgatttc aatgtcgtgc atctcggcga actggcgcat
     7681 atcgttgagg ttcagcgcat ggctgaagac ggtatggtgc gcgccaccag cgaggatcca
     7741 cgcttcggaa gcagttggca gatccggttg cgctttccac agcgcattcg ccaccggcag
     7801 tttcggcagg gagtgcggtg ttttcaccgt gtcgatacag ttaaccagca gacggtaacg
     7861 atcgccgaga tcaatcaggc tggcgacaat cgctggaccg gtttgggtat tgaagatcag
     7921 tcgggcagga tcgtccttac caccaatacc gagatgctga acgtcgagga tcggtttctc
     7981 ttctacggca atcgacgggc agacttccag catatgggag ccgagcacca ggtcattacc
     8041 tttctcgaag tgataggtgt agtcctccat aaaggaggtg ccgccctgca gaccggttga
     8101 catcaccttc atgatgcgaa gcagggcggc ggttttccag tcgccttcgc ccgcaaagcc
     8161 gtaaccctgc tgcatcagac gctgtacggc cagacctgga agctgtttca gaccgtgcaa
     8221 atcttcaaag gtggtggtga acgcgtggaa gccaccttgt tccaggaaac gcttcatccc
     8281 cagctcaata cgcgccgctt ccagcacgtt ctgtcgtttt tcgccgtgga tttgtgtggc
     8341 aggcgtcatg gtgtagcagc tttcgtactc atcgaccagc gcattaacat cgccgtcgct
     8401 gatggagttc accacctgca ccagatcgcc aaccgcccag gtattgacgg agaaaccgaa
     8461 cttgatctgt gcggcaactt tatcaccatc ggtgaccgct acttcacgca tgttatcgcc
     8521 aaaacggcag actttcagat gacgggtatc ctgtttagag accgcctggc gcatccagga
     8581 gccgatacgc tcatgggctt gtttatcctg ccagtgaccg gtaaccacgg catgttgctg
     8641 acgcatacgc gcgccaatga agccgaactc gcgaccgcca tgtgcagtct ggttcaggtt
     8701 cataaagtcc atatcgatac tgtcccacgg cagcgccgcg ttaaactggg tgtggaattg
     8761 cagcaacggt ttgttgagca tggtcaggcc gttgatccac attttggccg gggagaaggt
     8821 gtgcagccac accaccagac cagcgcaacg atcgtcgtaa ttcgcgtcgc ggcaaatagc
     8881 ggtgatttca tccggcgtgg tgcccagcgg tttcaacacc agtttgcagg gcagtttcgc
     8941 ttccgtattc agcgcattaa caacgtgctc ggcatgttgg gtgacctgac gcagggtttc
     9001 cgggccatac agatgctggc tgccaatgac aaaccacact tcataattat caaaaatcgt
     9061 cattatcgtg tccttataga gtcgcaacgg cctgggcagc ctgtgccggg gcggaagttg
     9121 gaagatagtg ttgttcggcg ctcatcgccc attgctgata gcggcgataa agctgttcaa
     9181 agcgttgtgc ctgttcgctg cgcggttgca gggttttctc taccgcactg gccatttttt
     9241 gctgggctga tgggatgtct gcgtgcactt tcgcggcgac ggcagcaaaa atcgccgcac
     9301 cgagcgcaca gcactggtca gaggcaacaa tttgcagcgg gcgattcagc acgtcgcagc
     9361 aggcctgcat aatgacctgg tttttccgcg cgatgccgcc cagcgccatc acgttattga
     9421 cggcgatccc ctgatcggta aagcactcca tgattgcgcg tgcgccaaag gcggtggcag
     9481 caatcaaacc gccgaacagc agcggagcgt cggtagcgag gttaagatcg gtaatcaccc
     9541 ctttcaggcg ttggttagcg ttcggtgtgc ggcggccgtt aaaccagtcg agcaccaccg
     9601 gcaggtgatc cagagacgga tttttggccc atgcttcggt cagcgccgga agcagttgtt
     9661 tctggctggc gttgatttgc gctttcagtt ccggatgctg ggcggcaagc tgttccagcg
     9721 gccagccgag tacgcgacca aaccaggcgt agatatcacc aaacgccgat tggcctgctt
     9781 ccagaccgat aaatccaggt accacgctgc catcaacctg accgcaaata cctttcactg
     9841 cccgctcgcc aacgctctgt ttgtcggcaa tcagaatgtc gcaggtggaa gtaccgataa
     9901 cttttaccag tgcgttaggc tgtgcgcctg cgccaactgc gcccatatgg cagtcaaacg
     9961 cgccgccgga aatcaccacg ctttcaggca ggccgagacg ctgcgcccat tccgggcata
    10021 aggtgcccac cggaatatcg gcagtccagg tgtcagtgaa cagcggggaa ggcaaatggc
    10081 gattgaggat cgggtccagc tcatcaaaga aactggctgg cggcaagcca ccccagcttt
    10141 cgtgccatag cgacttatgc ccggcgctgc aacgtccgcg acgaatatcc tgcgggcggg
    10201 tggtaccgga aagcagagct ggcacccagt cgcacagctc aatccacgat gcggcagatt
    10261 gcgccacggc ggtgtcctgg cgagtcacat gcaggatttt tgcccagaac cattcgctgg
    10321 aataaatacc gccaatgtag cgggagtagt caacgttgcc cggcgcgtgg cacaaacggg
    10381 taatctcttc cgcttcttca accgcagtgt ggtctttcca caatacgaac atcgcgttcg
    10441 ggttttcggc aaactccggg cgcagcgcca gcacgttacc gtcggcatca atcggtgcgg
    10501 gcgtcgagcc ggttgtgtca acgccaatcc cgaccacatc tgcgcgctgt tcgacgctaa
    10561 gctctgcaag cacggttttc agtgccgctt ccattgactc aatgtagtca cgcggatgat
    10621 gacggaactg gttattcggg gcatcacaaa attgcccttt ttgccaacgg ggataccact
    10681 ctacgctggt ggcgatctct tcaccgctgg cgcagtccac cgccaaagct cgcacagaat
    10741 cactgccaaa atcgaggcca attgcaatcg ccatcgtttc actccatcca aaaaaacggg
    10801 tatggagaaa cagtagagag ttgcgataaa aagcgtcagg taggatccgc taatcttatg
    10861 gataaaaatg ctatggcata gcaaagtgtg acgccgtgca aataatcaat gtggactttt
    10921 ctgccgtgat tatagacact tttgttacgc gtttttgtca tggctttggt cccgctttgt
    10981 tacagaatgc ttttaataag cggggttacc ggttgggtta gcgagaagag ccagtaaaag
    11041 acgcagtgac ggcaatgtct gatgcaatat ggacaattgg tttcttctct gaatggcggg
    11101 agtatgaaaa gtatggctga agcgcaaaat gatcccctgc tgccgggata ctcgtttaat
    11161 gcccatctgg tggcgggttt aacgccgatt gaggccaacg gttatctcga tttttttatc
    11221 gaccgaccgc tgggaatgaa aggttatatt ctcaatctca ccattcgcgg tcagggggtg
    11281 gtgaaaaatc agggacgaga atttgtttgc cgaccgggtg atattttgct gttcccgcca
    11341 ggagagattc atcactacgg tcgtcatccg gaggctcgcg aatggtatca ccagtgggtt
    11401 tactttcgtc cgcgcgccta ctggcatgaa tggcttaact ggccgtcaat atttgccaat
    11461 acgggtttct ttcgcccgga tgaagcgcac cagccgcatt tcagcgacct gtttgggcaa
    11521 atcattaacg ccgggcaagg ggaagggcgc tattcggagc tgctggcgat aaatctgctt
    11581 gagcaattgt tactgcggcg catggaagcg attaacgagt cgctccatcc accgatggat
    11641 aatcgggtac gcgaggcttg tcagtacatc agcgatcacc tggcagacag caattttgat
    11701 atcgccagcg tcgcacagca tgtttgcttg tcaccgtcgc gtctgtcaca tcttttccgc
    11761 cagcagttag ggattagcgt cttaagctgg cgcgaggacc aacgtatcag ccaggcgaag
    11821 ctgcttttga gcaccacccg gatgcctatc gccaccgtcg gtcgcaatgt tggttttgac
    11881 gatcaactct atttctcgcg agtatttaaa aaatgcaccg gggccagccc gagcgagttc
    11941 cgtgccggtt gtgaagaaaa agtgaatgat gtagccgtca agttgtcata attggtaacg
    12001 aatcagacaa ttgacggctt gacggagtag catagggttt gcagaatccc tgcttcgtcc
    12061 atttgacagg cacattatgc aagcattgct ggaacacttt attacccaat ctaccgtgta
    12121 ttcattgatg gcggtggtgt tggtggcctt tctggagtcg ctggcgctgg tcggtttgat
    12181 tctacccggt acggtgctga tggcggggct gggagcgctg attggcagcg gcgagttaag
    12241 tttctggcac gcctggctgg cagggattgt cggttgtctg ctgggcgact ggatctcctt
    12301 ctggctgggc tggcgtttta aaaagccgtt gcatcgctgg tcatttctga agaagaacaa
    12361 agcattactc gataaaactg aacatgcgtt gcatcaacac agcatgttca ccattctggt
    12421 cggtcgtttt gttggcccga cgcgtccgct ggtgccaatg gtggcgggaa tgctggatct
    12481 gccggtggct aaatttatta cgccgaatat tatcggctgc ctgctgtggc cgccgtttta
    12541 cttcctgccg gggattctgg cgggcgcggc gatcgatatt cctgccggaa tgcagagcgg
    12601 tgagtttaaa tggttgctgc tggtgacggc ggtgtttttg tgggttggtg gctggctgtg
    12661 ctggcggtta tggcgcagcg gcaaagcaac cgatcgtttg agtcattatt tgtcccgcgg
    12721 tcgtttgttg tggctgacgc cgttgatttc tgccatcggc gtggtggcgc tggtggtgtt
    12781 aattcgccat ccgttaatgc cggtgtatat cgatattttg cgtaaagtgg ttggagttta
    12841 gggcatagtc ttgtgtgggg cgcttgatgc ggacaaaacg cacccgtagg tcggataagg
    12901 cgttcacgcc gcatccgaca gtgcatatta acctgtaatt cccaacagcg ccgaagcact
    12961 cgccttaccg ctcaacaatt cttcggtctt accctgccag gcgatgcgcc cgtcggcgac
    13021 tactaccgag cgcgtggcaa tccgcgccgc atcttccaca ctgtgcgaca ccatcaatag
    13081 cgtcattttt tgctgctggc agctcgtgct caccagcgtc aacatctcct ggcgtagcgc
    13141 cggatcgagc gcagagaacg gttcatcgag caataaaatc ggctgctcgc gtaccagaca
    13201 acgcgctaac gccacgcgct gtcgctgacc gccggaaagc tcgcccggta accgcgccat
    13261 taaattatca atccccatct gacgggcaat cgcgtgcatt ttctcctgct gtgccgcatt
    13321 cagtttcaat cccggattta gccccagccc gatgttctgc gcgaccgtca ggtggctgaa
    13381 caggttgttc tcctgaaaca gcatcgacac cggacggcgt gacggcggcg tcgtggtgtg
    13441 atcaacgcca tcgatagtca gcgaaccgct ggctggcgtc agaaaaccgg caatcaaatt
    13501 cagcagggta cttttacccg cgccgcttgg cccgaggatc gccacctgct cgccgcgttc
    13561 tactgttaag ctaaaacgca tcggcaaatg gtggtaaagc caggtgatat cagtcagttt
    13621 taacatttcg ccccggtagt ttttcaatca cggtaaacag cagaaaacag agtagcagca
    13681 gaattaacgc ggtgaccgcg ccgtcctggc tgcgatagga gccaatttgc tggtagagat
    13741 aaaacggcag ggtacggaaa tcatcgttac cgaacaacgc caccacgcca aaatcaccaa
    13801 tcgacagcac gcaggcaaag gccagcgcct gcgccagtgg acgtttcagg gcgcgcagct
    13861 ccaccacttt taagcgtgac cagccttcaa tccccagcga ctgacataac atgctgtagc
    13921 gggcggtgat atcgcgcatc gggttttcca gcactttcag ggcgtagggg atcgccatta
    13981 acgcattggt gaaaatcaca atgccgtcag cagattgtgg caggccaatg gtgttgttga
    14041 gcagtaaaaa gaagcccgtt gccagcacaa tccccggcat agcgagaatc aacatgccgc
    14101 tcatctccag cgcctggccc gccagcattt tttgccgcgc ccgaagttcg cgactgctcc
    14161 atagcagcat catggtcagc actacgcaca atacacctgc cgccagcgca atacgcaacg
    14221 aggtccacag cgcctgccac agcaccggtt gtgccagcac ttccggcaac tggcgattta
    14281 ccccatcgac gatcaccgcc agcaacggtg gcagcaacag cagcagcgcc agcacaatta
    14341 acaccgtgtc gcaaatgcgg ctatgcagac gatcgtccgg gtcgcgccag ccttgcagca
    14401 gcgtggtgcc tggcgcaatg gccttactca atcgctgact caacagtacc agcccaaggc
    14461 aacacaccat ctggatcagc gccagcatcg ccgcgcgggc aggatcgtag tcgtaactca
    14521 gcgcctgata gattgccagc tcgatagtgg ttgcctgcgg accgccgccc agcgataaca
    14581 cggtggcgaa gctggcgaaa cagagcataa agataagcgc ggtaaccggc gggatttgtc
    14641 gccgtaacca cggccattcg acgaagcgga aaaaatgcca gccgcgcatc ccaagctggg
    14701 cggcaagctg acgctgctcg cctggaatgt tttccagtgc ctggagtaat aagcggctcg
    14761 ccatcggtag attaaaaaat acgtgcgcca gcaaaatacc ttgcaggccg tagggcgaaa
    14821 aggtccactc cagaccgagc gattggcaga gcgatgccag ccagccctgg cggccataga
    14881 cgctaagaat gccgaaaaca gcaaccagca ccgggaggat caaggtcatt gcgcacagac
    14941 gcaacagcgc cagccgaccc ggaaagcgcc tgcgatagag cgcgcgggcg aggaatatcg
    15001 cgggtacgac agagagtagc gctgagagaa acgcctgcca gaaggagaag cgcaccacat
    15061 gccacaggta gctgtcctgc cagactgccg accagtcacc ctgcggcgcg ttccaccaca
    15121 gggcgagaaa cgccgccagc gcaaccgcta ccaccagcgt ggcggcgctt acacccggaa
    15181 ttaaccagcc gggaattaac ggctgacggc gcgttgccat tcgctaatcc atgcctgacg
    15241 ttgtgccgcc acttcggctg gcgtgaactc cagcgtggtt gcgggtttgg tcaatttttc
    15301 aaaaccggca ggcagcgtga cgtttgccac cggatacatc cagttgccgg ttgggatcgc
    15361 attctggaaa gccggagaaa ccataaactg gaggaatttt tgcgccagct ccggctgctt
    15421 gctggcagcg gtgcgagcgg cgacttccac ttgcagatag tgaccttcgc tgaagttcgc
    15481 ggcggcgtag ttatctttct tctcttcgag aatgtgataa gccggagagg tggtgtaact
    15541 cagtaccaga tcgctttcac cttttaaaaa caggccgtag gcttcgctcc agcctttggt
    15601 gaccgtgacc gttttcttcg ccagtttctg ccaggcttgt ggggcgtcat cgccatagac
    15661 tttttgcatc catagcaaca gacccagccc cggtgtactg gtgcgcggat cctgataaat
    15721 cacccgccag ttttgatcgc tctcaaccag ttctttcagg ctttgtggcg ggtttttcag
    15781 tttgttcttg tcataaacga aggcgaagta gccataatca aacggtacga aagtgtcatt
    15841 attccagccg ccgggaacgt taacggcatc cgctgccaca ccgcttttgg caaacagtcc
    15901 ggttttactg gcggcgtcta acaggttgtt atccagcccc agcaccacat cggctttact
    15961 gtttttgcct tccatccgta gacggttgag aagcgaaacg ccatcttcca gcgccaccag
    16021 tttcagttcg caattacagt cggcttcaaa ggctttttta accaccggac caggccccca
    16081 gtcggcggcg aaggaatcgt aggtataaac agtcagaacg ggtttagcga aaacgggcgc
    16141 tgtgcacagc aacagcaggg gaagacattt ttttaacact ttgcacctca aaaaagagtg
    16201 gcaaaggact tgagaaggag cctcaaatcc cttcgccggc gttatccgga tcaggttcga
    16261 cgggtatttt ctcagcgcac gcgtccgcgt ggcaccccgt tgagaacggc gttagtgtag
    16321 tgattttgtt atcaaccagc aatccataga gaaggcgtat taaaaaagcc aatattcgtt
    16381 cagctatcaa gagcgtattt gcatagctgg agagcgttga gccgaaagaa tcccttagct
    16441 acagtcagaa tcggtaacgt aacagtttat tgcgtgaatg agcgattttt gacagtattc
    16501 ttattcagga cgcttattct gagtttttaa ctcagagtgg agctatcagc atgaaagtaa
    16561 gaaacccaga acagattagt atctctgcta gtaataccac taaagatcca ggccttacta
    16621 attctcagat tatcaggatg gttaacttgg tccaaaagac caagaatatg aatatctttg
    16681 aagagctatg ggagacgtta cgcaatctct ttcggtcaga taaacattct cagactgcgg
    16741 caaggcaaat tctgaaggat gcattttatt tccagaatag tgatgactac tcaaaatact
    16801 ttaccggggc tgtcgacggg aaggcgcgtg atcagttaac tcattggctg ataaaattta
    16861 atgaactcaa agaacacgca agagatccgg aaaatatggc cgcgaaggcc tcactctcgc
    16921 caaaagatac gttatgcgtc agttttttta ttggcgatga ggcgatattt actctgaagt
    16981 tgcagctgaa agagaacacc aggaccgggt gcattgacct gagtaatgct tactttaacg
    17041 gtgttgtcat ttgtggtatt gactgtctcg aggttgacat gagcaatgct gaaacgaaca
    17101 atagcagatg gtatgactga cctgcgcgag gttaacctga aaaagctgac ctttccggcc
    17161 agaacacggc tagaatgcgc cgttattgat taactcagaa tatgaaagag gggctggaat
    17221 gccccttctt taatcttatt tgcgacgcta atcagacgat gcataaaaca gagaaaatat
    17281 aaaagtaatg tattttatca ttccgtctga atataaaaaa tgcgctcatg accgccaaaa
    17341 ggcgcaagct actctggatc cggcggcgca aaccacgctg atttaaaatc aaaccagccg
    17401 agggtgttca tgcgcaggcc gcgcatactg cgttgtccct gaatgatcag ccagtggtgg
    17461 ataagtggca ccattgcttt gctggcgacc agttgttgac accagttcgc caggttcatt
    17521 tcgccattgc gccagcgggc ggcgtcggct tgccagtcga ttggaataca gtgttggagc
    17581 agcggtacct cgcacaggtg cgcgaacagc gaaaaatcca gcggcagcgt aaagttggcg
    17641 ctgttaagcc agatatcgct ctcgatctct ccttcatgcc actgatcgta gctgatctct
    17701 ttgatttcca gtgtgacctg gtggcttgcc agaatctgct gcatgatccc ggcaatcacc
    17761 cgatgctcac tgtgatcctg ataaaaggtc agggtgaggc tttccagacc agccggtttt
    17821 tcgctcttta tggtgcgggc atgatgccaa cgggggagca gtccataagc cgggaaccac
    17881 agttgctggt actgttcctc agcgaaatag accagattag ttggagaaag cacatagctt
    17941 acccagtccc tgacttgctg attcgccccg cgatgggtgc ggctatcgaa cagtaaatag
    18001 tagcaacctt cctccaggcg gctttcaatc tctttttcct cgccctgtgg accttttagc
    18061 atcagccctc cggctggctc gtcggcaatt tccggcagaa cccagacgtt aacttcatcg
    18121 attaatgccc ggtaaccgaa gaagtcatcg aatgcctgaa ttttcagttg attggtgctg
    18181 ttgcgaatca ccgcatacgg accggtgccg atgggatggc tggcaaagtt actgagggtt
    18241 tcccattcgc gcggcaggat catcgccgga acttgtccca gcagtaacgg caaccagcga
    18301 tccggctgcg tgaggtggat atccagcgtc cagggcgtcg gcgacacaat gtcagcaata
    18361 tgcgaataga gcggcagcgt attgattcgt tttaaagagg cgatcacgtc gtccatttcc
    18421 agttcacgac catgatgaaa atggactcct ggacgcaaaa agaaacgcca gtgaagcggt
    18481 gatatttgct gccagtggtg ggcgatgtct gcttccagtt ccccattttc ctcatttatg
    18541 cgcgttagcg aactgaagat ttgccgggcg atatgggttt cggaacggcg caatgcgctg
    18601 ccaggtagca gattacgcaa cggacgatag tagagcacgc gcaggatgtg ccgcccctgg
    18661 cggaagctgc ggcccagatg agaaaccagc atttgcctca cagtcgcttt gtcgccaact
    18721 aactgcacta gttggtcgat acggtcctgc tccagcaggt cttccgcccg ctgttgctga
    18781 agcgccagcc cggtatagag gaatgtcaga cgcgagcgtt taccacgccc gacttccgct
    18841 tcccacgtca gccagccgcg atcctgcatg gtgttgagca gggtgcgcat atgacgacgc
    18901 gagcagctca ataacgctgc cagttcgttg agcgttgtgt cctgcgattt accctcgcag
    18961 cattgccaca ggcggatgaa ctgttgttgc agacgagcag atggcataaa aggggaactc
    19021 ctgcgcaaaa gacagcaatt ttattttccc tatattaagt caataattcc taacgatgaa
    19081 gcaaggaggt gccccatgcg tcagttttat cagcactatt ttaccgagac agcgaagttg
    19141 tgctggttgc gttggttaag cgtcccacaa cgattaacca tgcttgaaga actgatgcag
    19201 tgggagggga gtcattctga ctactgattt gcggacatca tgtgtgactg agtattggtg
    19261 ttaatcgcca cgccagcagt gattatctgc tggctttttt ctttttggcg attgagtacc
    19321 ggacggtgta caaactaacg cataaacgcg ggttgcttcg cttcataagc cgcaatggcg
    19381 tcgtcgtgct gcaaggtaag cccaatgctg tccagaccgt tcatcatgca gtggcggcgg
    19441 aaggcatcaa tggtaaagcg ataggttttc tcacccgctt tcacctcttg cgcttccagg
    19501 tctacatcaa aatgaatccc cggattggct ttcaccagcg caaacagctc atccacttct
    19561 gcgtcgctta atttcaccgg cagcagctgg ttgttaaagc tattgccgta gaagatgtca
    19621 gcaaaactcg gcgcaatcac caccttaaaa ccgtaatcgg tcaatgccca cggcgcgtgc
    19681 tcacgcgagg aaccgcagcc gaagttttct cgtgccagca aaatggaagc gccctgatac
    19741 tgcgggaagt tcagcacgaa gtccgggttt ggctgttggc ctttttcatc cagaaaacgc
    19801 cagtcgttaa acagatgcgc gccaaaacca gtacgggtca ctttctgcaa aaactgtttc
    19861 gggatgattg catcggtatc gacattggcg gcatccagcg gaaccaccag gcctgtgtgt
    19921 ttgataaatt tctctgccat ggtgtgctcc ttatttaatg ttgcgaatgt cggcgaaatg
    19981 tccggtcacc gcagcggcgg cagccattgc cgggctgacc agatgcgtgc gcccgccgcg
    20041 cccctggcgg ccttcaaagt tacggttgct ggtggaggca caacgttcgc ccggattcag
    20101 ccggtcgttg ttcatcgcca gacacattga gcagccaggc aagcgccatt caaaaccggc
    20161 ttcaataaag attttatcca gaccttccgc ttccgcctgg gcttttaccg ggccagagcc
    20221 gggaaccacc agtgcctgca cgcctggcgc gacttttcgc cctttggcga tttccgctgc
    20281 cgcgcgtaaa tcttcaatgc gcgagttggt acatgaaccg ataaacactt tgtcgatagc
    20341 cacttcggtc agcggaatac cgggtttcag gcccatatag gccagcgctt tttctgctga
    20401 cgcacgttca accggatcgg caaacgaagc gggatcggga atattgtcgt tcacggaaat
    20461 cacctggcct ggattggtgc cccaggtgac ctgcggcgaa atctcttctg cttgcagagt
    20521 gacaacggta tcgaaagttg caccttcgtc ggtttgcagg gttttccagt aggcaactgc
    20581 atcgtcgaaa tctttgcctt tcggcgcatg caaacggcct ttgacatagt taaaggtggt
    20641 ttcgtccggt gcaaccagac cggcttttgc gcccatttcg attaccatat tgcacagggt
    20701 catacgacct tccatgctta aatcacggat tgcttcgccg caaaactcca ccacatgccc
    20761 ggtgccgcct gcgctaccgg ttttaccgat aattgccagc acgatatctt ttgcggtaat
    20821 gcccggcgcg gttttgccct ggacttcaat tttcatggtt tttgcgcggc cctgtttcag
    20881 ggtttgcgtt gccagtacgt gttcaacttc ggaagtgccg ataccaaagg ccagtgcgcc
    20941 aaacgcgccg tgggtggcgg tatgcgagtc gccgcagaca atggtcatcc ccggcaaggt
    21001 cacgccctgt tccggcccca ttacgtggac gatcccttga taggggtgat tcaggtcata
    21061 cagttcgaca ccaaattctt tgcagttttt gatcagttcc tgcatctgga tacgcgccat
    21121 ttcaccgcag gcattaatgt ctttggtctg ggtagagacg ttgtgatcca tggtagcgaa
    21181 ggttttgccc ggctgacgta ccgggcgacc gtgggcgcgc agaccatcga acgcctgcgg
    21241 tgaggtcact tcatgcacca ggtggcggtc gatatataac agtggggttt cgttttcggc
    21301 ttcgtacaca acgtgagcgt cgaacaattt ttcgtataac gtcttagcca tgattacacc
    21361 ccttctgcta catagcgggc aatgatatcg cccatttcat cggtactaac ggcggcagcg
    21421 ccacgggcta aatccccggt gcgaatgcct tcttctaatg cgcggttaat ggcgcgttca
    21481 atggcggaag ccgcatcatc ggcatccagg ctgtaacgca gcagcagtgc cagcgacaga
    21541 atttgcgcaa tcgggttggc gatgttttta cctgcgatat ctggtgccga gccgcccgcc
    21601 ggttcataca gtccaaaacc ttgctcgttc aggctggcgg aaggcaacat ccccatcgag
    21661 ccagtgatca ttgcgcactc gtcagacaga atgtcgccaa acaggttgga gcacagcaga
    21721 acgtcaaact gagatggatc tttaatcagc tgcatggtgg cgttgtcgat gtacatatgc
    21781 gccagttcga tatccgggta ttccgtggcg atctcgttga cgatttcacg ccataaaata
    21841 gaggattgca gcacgttagc tttgtcgatt gaggtgactt tgtggcgacg cttgcgagca
    21901 gattcaaacg cgatgcgggc gatacgttca atctcaaaac ggtgatacac ctcggtatca
    21961 aacgcttttt catattgtcc gctaccttcg cggccttttg gctgaccgaa atagatgccg
    22021 ccggtcagtt cgcgcacgca caggatgtcg aagccgttag cggcaatgtc agcacgcagc
    22081 ggacagaatg cttccagccc ctgatacagt tttgccggac gcaggttgct gaataatttg
    22141 aagtgcttac gcaaaggcaa cagtgcgccg cgttctggtt gctggtccgg tggtaaatgt
    22201 tcccacttcg ggccgcctac cgagccaaac agcacggcat cggcttgctc acaaccttca
    22261 accgtcgcag gcggcagcgg ctgcccgtgg ttatcaatgg ctgcgccgcc tacatcgtaa
    22321 tggctggtgg tgatgcgcat cgcaaagcgg ttgcgcacgg catccagcac tttcagcgcc
    22381 tgggtcatta cttccggacc aattccgtct cccggcaata cggcaatatg gtaattcttc
    22441 gacatcacac ggtttccttg ttgttttcgt tgtgttgagc tttgcgttgc aactcttttt
    22501 cgacttctgc ggcgcgccag atattgttca gcacgtgcac catggctttg gcggaggact
    22561 cgacaatatc ggtggccagg ccgacgccgt ggaagcggcg accgttgtaa tttgcgacga
    22621 tatctacctg acccagcgca tctttaccgt ggcctttggc ggtcaggctg tatttcacca
    22681 gttcgacgtt atagtcagtg atgcggttta tcgcctgata gacggcatcg accggaccgt
    22741 taccgttggc ggcttctgct ttgacttctt cgccgcaggc cagtttgacg gcggcagtgg
    22801 caatatcgtt agaacctgac tgcacgctga agtaatccag acggaaatgc tccggctctt
    22861 cttgctgctt accgatgaag gccagcgcct ccagatcgta atcaaacacc tgaccttttt
    22921 tgtccgccag cttcaggaag gcgtcgtaca aattgtctaa attatattca ctttctttat
    22981 accccatctc atccatgcga tgtttcaccg ccgcacgccc cgaacgagag gtcagattca
    23041 gctggatttg gttcagacca atagattctg gtgtcatgat ttcgtagttt tcgcggtttt
    23101 tcagcacgcc atcctggtgg ataccggagg agtgagcgaa tgcgccgctg ccaacaatgg
    23161 ctttgtttgc cgggatcggc atattacaaa tctggctaac taactggctg gtgcgccata
    23221 tctcctggtg attaatggcg gtgtggacgt tgagaatatc cttacgaact ttaatcgcca
    23281 tgatgacttc ttccagcgaa cagttaccgg cacgctcgcc gatcccgttc attgcacctt
    23341 ccacctggcg tgcaccggca tgtaccgccg ccagtgagtt gccgaccgcc aggcctaaat
    23401 cgtcgtgggt atgtacggaa ataatggctt tgtcgatgtt aggtacgcgt tcatacagac
    23461 cgctgatgat tccggcgaac tcaaacggca tggtgtagcc cacggtgtcc ggaatgttga
    23521 tggtggtggc accggcatta atcgccgctt cgaccactcg cgccagatcg gcaatggggg
    23581 tacgcccggc atcttcgcaa gaaaattcaa catcatcggt gtaattacgg gcgcgtttca
    23641 ccatatagat agcgcgttcg atcacttcat ccagcgtgct gcgcagcttg gtggcgatgt
    23701 gcattggcga agtggcaata aaggtatgaa tacggaaggc ttcggcgact ttcagggatt
    23761 cggccgccac gtcgatatct ttctccacgc agcgagctaa cgcgcatacg cggctgtttt
    23821 taacctggcg ggcgatggtt tgcactgatt caaaatcgcc cggcgaagag acggggaaac
    23881 cgacttccat cacgtcaaca cccatacgct caagggccag cgcaatttgc agtttttctt
    23941 tcacactcaa gcttgcctgt aacgcctgtt caccgtcgcg caatgtggta tcgaaaataa
    24001 tgacttgctg gctcatggtt tgggtccttg tctcttttag agcgcctcgc ttcgggcata
    24061 aaaaaacccg cgcaatggcg cgggtttttt gtttgactgc gtgctggctt aatgctggat
    24121 gccgctcact cgtctaccgc gcaaagaaga tgcgtttagt agtagtagac cgataaagcg
    24181 aacgatgtga gtcattaaat cagctccaga tgaatgcgat atgcttttag agttactgga
    24241 tacaaaaacg gatgtcaacc ctgacgcaat aaaaacgtcc cgccagcatg agttctgcat
    24301 ccgtaaaatt agctaattgt gctgcgctca cgataatcat cgatattaat ttctgtttaa
    24361 tcgcacacac ttttcatgga ttgacatatt tcagcatgat tttttgcatc taatcaatga
    24421 actatgaggg gataaagctt acttgtcacc cttcttatcc tgatgatttg tcttaaatta
    24481 tttatttcgc gaatattttt atattcattt ggtgaatatt attgttcgat taatgttaag
    24541 gattaacgta ttaaatatat aaattaatca ttaaattcgt acgtttaatc ccttttccaa
    24601 atgattgagt attcgcggta gttatgatta gattgtttac gcaacaaaaa cattatggat
    24661 tattatgctg tggtaaatgc cgctttccac ggcaatggat tctgttttta taagaacccg
    24721 tatctttatg ttttcctaat tttactcatt ttcctt
//
LOCUS       DAPGOF010000065        24615 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.065, whole genome shotgun sequence.
ACCESSION   DAPGOF010000065 DAPGOF010000000
VERSION     DAPGOF010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.065"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            431..1570
                     /gene="wza"
                     /locus_tag="RHV13_002262"
     CDS             431..1570
                     /gene="wza"
                     /locus_tag="RHV13_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HDV1241791.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            1576..2019
                     /gene="wzb"
                     /locus_tag="RHV13_002263"
     CDS             1576..2019
                     /gene="wzb"
                     /locus_tag="RHV13_002263"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HDV1241792.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            2022..4184
                     /gene="wzc"
                     /locus_tag="RHV13_002264"
     CDS             2022..4184
                     /gene="wzc"
                     /locus_tag="RHV13_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HDV1241793.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            4362..5201
                     /gene="wcaA"
                     /locus_tag="RHV13_002265"
     CDS             4362..5201
                     /gene="wcaA"
                     /locus_tag="RHV13_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HDV1241794.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            5204..5692
                     /gene="wcaB"
                     /locus_tag="RHV13_002266"
     CDS             5204..5692
                     /gene="wcaB"
                     /locus_tag="RHV13_002266"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HDV1241795.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            5689..6906
                     /gene="wcaC"
                     /locus_tag="RHV13_002267"
     CDS             5689..6906
                     /gene="wcaC"
                     /locus_tag="RHV13_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HDV1241796.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIVLFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEDVLQLVQLSKPEIAQAIFGTTLAGFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            6881..8098
                     /gene="wcaD"
                     /locus_tag="RHV13_002268"
     CDS             6881..8098
                     /gene="wcaD"
                     /locus_tag="RHV13_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HDV1241797.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGVGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            8109..8855
                     /gene="wcaE"
                     /locus_tag="RHV13_002269"
     CDS             8109..8855
                     /gene="wcaE"
                     /locus_tag="RHV13_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HDV1241798.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQADDISFEWIVVDGGSN
                     DGTREYLENLNSIYNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            8871..9419
                     /gene="wcaF"
                     /locus_tag="RHV13_002270"
     CDS             8871..9419
                     /gene="wcaF"
                     /locus_tag="RHV13_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HDV1241799.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            9445..10566
                     /gene="gmd"
                     /locus_tag="RHV13_002271"
     CDS             9445..10566
                     /gene="gmd"
                     /locus_tag="RHV13_002271"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HDV1241800.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            10569..11534
                     /gene="fcl"
                     /locus_tag="RHV13_002272"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             10569..11534
                     /gene="fcl"
                     /locus_tag="RHV13_002272"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HDV1241801.1"
                     /translation="MSKQRIFIAGHRGMVGSAIKRQLEQRGDVDLVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            11537..12016
                     /gene="gmm"
                     /locus_tag="RHV13_002273"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             11537..12016
                     /gene="gmm"
                     /locus_tag="RHV13_002273"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HDV1241802.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEDLILPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            12013..13236
                     /gene="wcaI"
                     /locus_tag="RHV13_002274"
     CDS             12013..13236
                     /gene="wcaI"
                     /locus_tag="RHV13_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HDV1241803.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            13239..14675
                     /gene="cpsB"
                     /locus_tag="RHV13_002275"
     CDS             13239..14675
                     /gene="cpsB"
                     /locus_tag="RHV13_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HDV1241804.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            14868..16238
                     /gene="cpsG"
                     /locus_tag="RHV13_002276"
                     /gene_synonym="manB"
     CDS             14868..16238
                     /gene="cpsG"
                     /locus_tag="RHV13_002276"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HDV1241805.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDATKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKEKTLGELVRDRMVAFPASGEINSKLAQPAEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            16293..17687
                     /gene="wcaJ"
                     /locus_tag="RHV13_002277"
     CDS             16293..17687
                     /gene="wcaJ"
                     /locus_tag="RHV13_002277"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HDV1241806.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            17689..19167
                     /gene="wzxC"
                     /locus_tag="RHV13_002278"
     CDS             17689..19167
                     /gene="wzxC"
                     /locus_tag="RHV13_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HDV1241807.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGIVVCVAVFLLSDLI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLMVEVKRQFCRSEKMKMLLRAG"
     gene            19347..20627
                     /gene="wcaK"
                     /locus_tag="RHV13_002279"
     CDS             19347..20627
                     /gene="wcaK"
                     /locus_tag="RHV13_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HDV1241808.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPQAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRMLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDGGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLNEAVSRERQTGMQMVQSVLERIGEAK"
     gene            20624..21844
                     /gene="wcaL"
                     /locus_tag="RHV13_002280"
     CDS             20624..21844
                     /gene="wcaL"
                     /locus_tag="RHV13_002280"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HDV1241809.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFHADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVDMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            21855..23249
                     /gene="wcaM"
                     /locus_tag="RHV13_002281"
     CDS             21855..23249
                     /gene="wcaM"
                     /locus_tag="RHV13_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HDV1241810.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINNYNPHD
                     WIASFKQAFSEGQTVVVPAGLVCDNINTGIFIPAGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLNV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSNILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNEKGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            23424..24317
                     /gene="galF"
                     /locus_tag="RHV13_002282"
     CDS             23424..24317
                     /gene="galF"
                     /locus_tag="RHV13_002282"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HDV1241811.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
BASE COUNT         6052 a         6126 c         6698 g         5739 t
ORIGIN      
        1 attgaatgac acttaatata attcttaaaa atagccaatt gccgaattgt taccttgcct
       61 gctattccgt tagctgtaac acttcctcct gcattattgg agagccaata ttcaattacc
      121 ataatgtcac cttatattcg gaagttaatt tccgcaataa ccctttctca tcaacgattg
      181 tatgcagaga gggtttgaaa aattaagcta aatcagtgta ttggtagcta aaaagccagg
      241 ggcggtagcg tgtctggatg cctgaaagac cagtctgaat tattctgtca atagcctgcg
      301 gatgaaatca atttttttag gactgatgcc agttaaattt gtattcaatt aactgcaagg
      361 taaatgtgca tggcgacatt gctattaata gtgcacagga taattactct gccaaagtga
      421 taaataaaca atgatgaaat ccaaaatgaa attgatgcca ttattggtgt cagtaacctt
      481 gataagcggt tgcacagtac ttccgggcag caatatgtcg acgatgggca aagacgtcat
      541 caaacagcag gacgctgatt tcgatctcga caaaatggtg aatgtttatc cgttgacccc
      601 gcgcctgatt gatcaattac gtccacgacc caacgtagcg cgccccaata tgacgctgga
      661 aagtgagatc gcgaattacc agtatcgcgt cgggccgggg gacgttctga atgtcaccgt
      721 ctgggatcac ccggaactca ccacgccagc cggtcagtac cgcagctcca gtgacaccgg
      781 caactgggta cagcctgacg gcactatgtt ttacccgtat atcggcaagg ttcacgtagt
      841 cgggaaaacg ctcgctgaaa tccgcagtga tattaccggg cgcttagcga cgtacatcgc
      901 tgacccgcag gtggacgtta atatcgccgc cttccgctca caaaaggcct atatctccgg
      961 acaggtgaat aaatccggtc aacaggcgat caccaacgta ccactgacca ttctcgacgc
     1021 catcaacgcc gcaggtggcc tgaccgacac cgctgactgg cgcaacgtgg tgctaacaca
     1081 caatggtcgt gaagaacgca tttctttgca ggcgctgatg caaaacggcg acctcaatca
     1141 gaaccgactg ctttatcccg gcgatattct ctacgtgccg cgtaatgatg atctgaaagt
     1201 gtttgtgatg ggtgaagtga agaaacagag caccctgaaa atggacttta gcggcatgac
     1261 cctgactgaa gccctgggca atgccgaagg tatcgacatg accacctcca acgccagcgg
     1321 catctttgtc attcgtccgc tgaaaggcga gggcgggcgt aatggcaaga ttgccaatat
     1381 ctaccagctg gatatgtccg atgcgacgtc gctggtgatg gcgacagaat tccgcctgca
     1441 accttatgac gtggtgtatg tcaccaccgc cccggtttcc cgctggaacc gtctgatcaa
     1501 tcagttgctg ccaactatta gcggtgtccg ttacatgacg gatacagcca gcgacattca
     1561 taactggtaa tcgtcatgtt taacaacatc ttagttgtct gtgtcggcaa tatttgccgt
     1621 tccccgacgg cggaacgctt actgcaacgt tatcacccgg agctgaaagt ggagtccgct
     1681 ggactcggcg cgctggtcgg taagggcgct gatcctaccg ctatcagcgt cgccgcagaa
     1741 catcaactgt ctctggaagg tcactgtgca cggcaaatca gccgccgcct gtgtcgcaac
     1801 tatgacctga ttttgaccat ggaaaagcgc catatcgaac gcttatgcga gatggcacct
     1861 gagatgcgcg gcaaagtgat gctgtttggt cactgggata acgaatgtga aatccccgat
     1921 ccgtatcgca aaagccggga aacgtttgca gcggtgtaca cattacttga acggtctgcc
     1981 cgccagtggg cgcaggcatt gaacgcagag caggtataag aatgacagaa aaagtaaaac
     2041 aacatgccgc tccggtaacg ggcagtgatg aaatcgatat tggtcgcctg gtcggcaccg
     2101 tcattgaagc gcgctggtgg gtgattggca tcaccgctgt atttgccctc tgtgctgtgg
     2161 tttacacttt cttcgccacg ccgatttata gtgccgacgc actggtacaa atcgaacaaa
     2221 acagcggcaa ttcgttagtg caggacattg gctcggcatt agccaacaaa ccgcctgcat
     2281 cggacgccga gatccagttg attcgttcgc gtctggtgct tggtaaaacg gtggacgatc
     2341 tagacctcga tattgcggtg agcaaaaaca cgttcccgat tttcggtgcg ggctgggatc
     2401 gtttgatggg acgtcagaac gagacggtga aagtgaccac ctttaaccgc ccgaaagaga
     2461 tggcggacca ggtgtttacg cttaatgtgc tggatgacaa aaactacact ctgagcagtg
     2521 atggcggctt tagcgcccgt gggcaagcgg gccagatgct gaaaaaagaa ggcgtcacgc
     2581 tgatggttga agccattcac gcccgcccgg gcagtgagtt taccgtcacc aaatactcca
     2641 cgttggggat gatcaaccaa ctgcaaaaca gcctgacggt aacggagaac ggcaaagacg
     2701 caggcgtact gagcctgact tataccggtg aagatcgcga acagatccgc gacattctta
     2761 acagcatcgc ccgtaactat caggaacaaa atattgagcg caaatcggcg gaagcgtcga
     2821 aaagcctcgc tttcctcgcg caacagttac cggaagtacg tagccgcctc gatgttgccg
     2881 aaaacaaact gaatgccttc cgtcaggata aagattctgt tgatctgccg ctggaagcga
     2941 aagcggtgct cgattcgatg gtgaacatcg acgcgcagtt gaacgaactg acctttaaag
     3001 aggcggaaat ctccaagctg tacaccaaag ttcaccccgc gtaccgcacg ctgctggaga
     3061 aacgtcaggc gctggaagac gaaaaagcca aacttaatgg tcgcgtaacg gcgatgccga
     3121 aaacccagca ggaaattgtc cgtctgaccc gcgatgtcga gtctggtcag caggtctata
     3181 tgcaactgct gaataaagag caggagctga aaatcaccga agccagcacc gtcggcgatg
     3241 tgcgcattgt tgacccggca atcacccagc caggtgtact gaaaccgaag aaagggctga
     3301 ttatccttgg ggcgattatt cttggcctga tgctctctat cgtgggtgtg ctgctgcgct
     3361 cgttgtttaa tcgcggcatc gaaagcccgc aggtgctgga agaacacggt atcagcgtct
     3421 atgccagcat cccgttgtcg gaatggcaga aagcgcgcga tagcgtcaaa accatcaaag
     3481 ggattaaacg ctataaacag agccagctac tggcggtggg gaatccaacc gatctggcga
     3541 ttgaagccat ccgcagtctg cgtaccagtc tgcacttcgc gatgatgcag gcgcagaaca
     3601 atgtgttgat gatgaccggg gttagcccgt caatcggtaa aacctttgtc tgcgccaacc
     3661 tggcggcggt gatcagccag accaataaac gcgtgttgtt gatcgactgc gatatgcgca
     3721 aaggctacac ccacgagctg ttgggcacca ataacgtcaa tggcctgtcg gaaattctga
     3781 ttggtcaggg cgatattact accgcagcta aaccgacctc tattgccaaa tttgacctga
     3841 tcccgcgcgg tcaggtgccg ccaaatcctt ctgaactgtt gatgagcgaa cgctttgccg
     3901 aactggtgaa ctgggcgagt aaaaactatg acctggtgtt gattgatacg ccgccgattc
     3961 tggcagtgac cgatgcggca attgttggtc gtcatgtcgg aaccacatta atggtggcgc
     4021 gttatgcggt caacacattg aaagaagtgg aaaccagtct gagccgcttt gagcaaaacg
     4081 gtattccggt gaaaggggtg attctgaact ccatcttccg ccgcgccagc gcgtatcagg
     4141 attatggcta ttacgaatac gaatataagt cggatgcgaa ataacgacat ccgctgtcgg
     4201 atgcgatgct gacgcatctt atccgaccta ccttcacgcc gcatccggca agcaaaccag
     4261 ctccgtaggc cggataaggc gctcacgccg catccggcaa cagaacccgt aaacaagctt
     4321 ataaaccggc agaacatctc gatactccgg gagaacaacc tatgaaagac aatccgctga
     4381 tctcaatcta tatgccgacc tggaaccgcc aacaactggc gattcgggcg ataaaatcgg
     4441 tcctgcgcca ggactacagc aactgggaga tgatcatcgt ggatgattgc tccacctcct
     4501 gggaacaact gcaacagtac gtcaccgccc tcaacgatcc gcgtattacc tacattcaca
     4561 acgacattaa cagcggggcg tgcgcggtac gtaaccaggc gattatgctg gcgcaagggg
     4621 aatacattac cgggatcgat gacgatgatg aatggacgcc caaccgcctg agcgtcttcc
     4681 tcgcccataa acagcaactg gtcactcacg cctttttgta cgctaacgac tacgtatgcc
     4741 agggagaagt ttattcccaa ccggcaagct taccactgta tcccaaatcg ccgtactcac
     4801 gccgcctgtt ctataaacgc aatatcattg gcaatcaggt ttttacctgg gcgtggcgtt
     4861 tcaaagagtg cttgttcgat accgaactga aagcagcaca ggattacgac atcttcctgc
     4921 ggatggtggt ggagtatggc gaaccgtgga aagtggaaga ggcgacgcag atcctgcata
     4981 tcaatcacgg tgaaatgcag atcacctcgt caccgaaaaa attctccggg tacttccact
     5041 tttaccgcaa gcacaaagat aaatttgacc gtgcaagcaa aaaatatcag ctgtttaccc
     5101 tctaccagat ccgcaataaa cgtatgacct ggcgcacttt gctgacgctg ctttcggtgc
     5161 gtaacggcaa acgtctggct gatggtattc gggggcgcta aagatgctgg aagatctgcg
     5221 cgccaacagc tggagtttac gtccgtgctg catggttcta gcctatcgcg tcgctcattt
     5281 ttgttcggtg tggcgcaaaa agaacgtcct caacaatctg tgggcggccc cgctgctggt
     5341 gctgtatcgc attatcaccg aatgcttttt cggttatgaa atccaggccg ccgcgaccat
     5401 tggccgccgc tttactatcc atcacggtta cgccgtagtg atcaataaaa acgtggtagc
     5461 gggggatgat ttcaccattc gccacggcgt cactatcggc aatcgtggtg ccgataacat
     5521 ggcatgtcca catattggca acggcgtcga actcggtgcc aacgttatta ttcttggtga
     5581 tatcacgctt ggtaacaacg tcaccgtggg tgcgggcagc gtggtgctcg actctgtccc
     5641 ggacaacgcg ctggtggtgg gagaaaaagc gcgagtgaag gtaattaaat gaatattttg
     5701 caatttaatg tgcgactggc ggaaggcggg gcagcaggtg tggcgttaga tctccaccag
     5761 cgtgcgctgc aacaggggct agcgtcacat tttgtctacg gttacggcaa aggcggcaaa
     5821 gagagcgtca gccatcaaaa ctatccgcag gtcatcaaac atacgccgcg gatgactgca
     5881 atggcgaata ttgtcctgtt tcgtctgttt aatcgcgatc tgtttggcaa tttcaatgag
     5941 ttatatcgca ccattactcg cacaccgggt ccggtggtcc tgcattttca tgtgctgcac
     6001 agctactggc taaatcttaa gagcgtggtg cgcttttgcg aaaaagtgaa aaaccataaa
     6061 ccggacgtca ctctggtctg gacgctgcac gaccactgga gtgttaccgg acgctgcgcc
     6121 tttaccgacg gttgcgaagg ctggaaaaca ggctgccaga aatgcccgac cttaaataat
     6181 tatccgccgg tgaagattga tcgggcgcac cagctggtgg cgggcaaacg ccagttattc
     6241 cgtgagatgc tggcgctggg ctgtcagttt atttccccca gccagcatgt ggctgacgct
     6301 ttcaatagcc tgtacggtcc agggcgttgc cggattatca ataacggtat tgatatggca
     6361 accgaagcga ttctggcgga tctgcctccg gtgcgcgaaa cccagggtaa gccgaaaatc
     6421 gcggtggtgg cgcatgacct gcgttacgac ggcaagacta accagcaact ggtgcgtgag
     6481 atgatggcgc tgggcgacaa aatcgaactg catacctttg gtaagttctc gccgttcacc
     6541 gctggtaacg tggttaatca cggctttgaa accgacaaac gtaagctgat gagcgcgctc
     6601 aatcagatgg atgcgctggt attcagttct cgcgtcgata actacccgct gattttgtgt
     6661 gaggcgctat cgattggcgt gccggtgatt gccacccata gcgacgcggc gcgggaagtg
     6721 ctgcaaaaat ccggcggtaa aaccgtcagc gaagaagacg tgctgcaact ggtgcagtta
     6781 agcaaaccgg aaatcgcgca ggcgatattt ggtaccacgt tggccgggtt cagccaacgt
     6841 agccgcgccg cctacagtgg acaacagatg ctggaggagt atgtcaactt ctatcagaat
     6901 ctgtagctac ctgctgctgc cgctgatcta cctgctggtt aacgtcaaaa tcgcccagct
     6961 tggtgaaagt ttccccatca ctatcgtcac ttttttacct gtcttgttac tgctgttttt
     7021 agaacgcatc agcgttaaaa aattgatgat tgccttaggg gttggcgcgg ggctcacggc
     7081 gttcaactat ctgtttggtc agtcgttgga tgccagtaaa tacgtcactt caaccatgct
     7141 gtttgtctat attgtgatca ttattggcat ggtgtggagt attcgcttta aaacgatttc
     7201 gccacacaac catcgcaaga tattacgttt cttttatctg gtggtcgggc tggtggtggc
     7261 gctggcggcg gtggagatgg cgcaaattat cctcaccggt ggcagcagta ttatggagtc
     7321 gatttcgaaa tatctgattt atagcaacag ctatgtactg aatttcatta aattcggcgg
     7381 caagcgcacg acagcacttt atttcgaacc ggcatttttc gctctggcat taatctcaat
     7441 ttggctcagc atcaaacagt ttggtatcaa aacgcctaaa acagatgcta tgattctcgc
     7501 agggataata ttatccggat cgttttcagg ggttatgacc tttatcctgt tttatttgct
     7561 ggagtgggca ttccaatatc tgaataaaga ggcgattaag aaaaagttac cgttagcatt
     7621 gatttctctg gctgtattcc tggttggtgt ggtaatcgcg tttccttata tttccacccg
     7681 tctgggcgat ttaggtacgg aaggatcgtc atcgtattat cgtattgtcg gtccgctggt
     7741 gatggtcggt tattctttga cccatattga cggtgtagtc agatttggct cactttatga
     7801 atatgtcgca tcattcggaa tatttaacgg tgcggatgtc ggaaaaacca tagacaatgg
     7861 tttgtatctg ctgattattt atttttcctg gttcgcggtg tttttatcgc tgtggtacat
     7921 ggggaaagtg ataaaaatga tgatcaacgc ttttggtgat aaccgcaatt ttcgcgtgca
     7981 attatatctt tttactccgg tttcgctgtt ttttaccggt tcgatattta gcccggaata
     8041 tgcattttta atcgtctgtc cgtttatttt gcgaaaagcg ttaaatatta cgaggtaaga
     8101 ataagaacat gttgcttagc ataatcactg tcgcgtttcg taacctcgaa gggatagtca
     8161 aaacacatgc ctcgctggcg catctggcgc aggcggacga tatcagcttc gaatggattg
     8221 ttgtcgatgg cggttccaac gacggtaccc gtgagtatct ggagaatctc aatagtatct
     8281 ataacctacg ctttgtcagc gagccagata acggtatcta cgacgccatg aacaagggca
     8341 ttgcgatggc acaaggcaag ttcgcgttgt ttctcaactc gggcgatatt tttcatcagg
     8401 atgccgcaaa ttttgttcgt aagttaaaaa tgcaaaaaga taacgtgatg atcaccggcg
     8461 atgcgctgct ggattttggc gacgggcata aaattaagcg tagtgctaaa ccgggttggt
     8521 atatttatca cagcctgccc gccagccatc aggcgatatt tttcccggta tccggtttga
     8581 aaaaatggcg ttatgacctg gaatataaag tttcttctga ttacgcgctg gcagctaaaa
     8641 tgtataaagc aggttatgca tttaaaaaac tcaatggcct ggtgtctgaa ttttccatgg
     8701 gtggggtatc aaccaccaat aatatggaat tgtgtgctga cgccaaaaaa gtccaacggc
     8761 agatattaca tgtgcctggc ttctgggcag aattatcctg gcatttacgc caacgtacta
     8821 cctcaaagac gaaagcctta tataacaaaa gctgaatata agggaaaacg atgcaggatt
     8881 taagcggttt ctcggtgccg aaagggttcc ggggcggcaa cgctattaaa gtgcaattat
     8941 ggtgggcagt acaggcaaca atatttgcct ggtcgccaca agtattgtat cgctggcggg
     9001 cttttttatt acgtttattc ggagcaaaaa taggaaaaaa cgtagttatt cgtccgtcag
     9061 taaaaattac ctatccgtgg aaattaacct taggtgatta cgcgtgggtc ggcgatgacg
     9121 tcaatttata taccctcggt gaaataacca ttggcgcaca ttcggtgata tcgcaaaaaa
     9181 gttatttatg caccggtagc cacgaccatg caagtcaaca tttcaccatt aacgccacgc
     9241 ctattgtgat tggcgagaaa tgctggctgg caaccgatgt ctttgttgcc ccaggcgtca
     9301 caatcggcga cggcactgtc gtgggtgcac gaagcagtgt ttttaaatcg cttccggcaa
     9361 atgtggtttg ccgggggaat cccgcagtgg tgatacgcga acgcgttgaa actgaataaa
     9421 ttcaaaaata cagaggaata agacatgtca aaagtcgctc tcatcaccgg tgtaaccgga
     9481 caagacggtt cttacctggc agagtttctg ctggaaaaag gttacgaggt gcatggtatt
     9541 aagcgtcgcg catcgtcatt caacaccgag cgcgtggatc acatttatca ggatccgcac
     9601 acctgcaacc cgaaattcca tctgcattat ggcgacctga gtgatacctc taacctgacg
     9661 cgcattttgc gtgaagtaca gccggatgaa gtgtacaacc tgggggcaat gagccacgtt
     9721 gcggtctctt ttgagtcacc agaatatacc gctgacgtcg acgcgatggg tacgctgcgc
     9781 ctgctggagg cgatccgctt cctcggtctg gaaaagaaaa cccgttttta tcaggcttcc
     9841 acctctgaac tgtacggtct ggtgcaggaa attccgcaga aagaaaccac gccgttctac
     9901 ccgcgatctc cgtatgcggt cgccaaactg tacgcctact ggatcaccgt taactaccgc
     9961 gaatcctacg gcatgtacgc ctgtaacggt attctcttca accatgaatc cccgcgccgc
    10021 ggtgaaacct tcgttacccg caaaatcacc cgcgcaatcg ccaatatcgc ccaggggctg
    10081 gagtcgtgcc tgtacctcgg caatatggat tccctgcgtg actggggcca tgccaaagac
    10141 tacgtaaaaa tgcagtggat gatgctgcaa caggaacagc cggaagattt cgttattgct
    10201 accggcgttc agtactccgt acgtcagttc gtggaaatgg cggcagcaca gttgggcatc
    10261 aaactgcgct ttgaaggcac gggtgttgaa gagaagggca ttgtggtttc cgtcaccggg
    10321 catgacgcgc cgggcgttaa accgggtgat gtgattatcg ccgttgatcc gcgttacttc
    10381 cgtccggcag aagttgaaac gctgctcggc gacccgacca aagcgcacga aaaactgggc
    10441 tggaaaccgg aaatcaccct cagagagatg gtgtctgaaa tggtggctaa tgacctcgaa
    10501 gcggcgaaaa aacactctct gctgaaatct cacggctacg acgtggcgat cgcgctggag
    10561 tcataagcat gagtaaacaa cgcattttta tcgctggcca tcgtgggatg gtcggttctg
    10621 ccatcaagcg gcagctcgaa cagcgcggtg atgtggatct ggtattacgt acccgcgacg
    10681 agctgaacct gctggacagc cgcgccgtgc atgatttctt tgccagcgaa cgcattgacc
    10741 aggtctatct ggcggcggcg aaagtgggcg gcattgttgc caacaacacc tatccggcgg
    10801 atttcatcta ccagaacatg atgattgaga gcaacatcat tcacgccgcg catcagaacg
    10861 acgtgaacaa actgctgttt ctcggatcgt cctgcatcta cccgaaactg gcaaaacagc
    10921 cgatggcaga aagcgagtta ttgcagggca cgctggagcc gaccaacgag ccttacgcca
    10981 ttgccaaaat cgccgggatc aaactgtgcg aatcttacaa tcgccagtac ggacgagatt
    11041 accgctcggt catgccgacc aacctgtatg gcccgcatga caacttccac ccgagtaatt
    11101 cgcatgtgat cccagcattg ctgcgtcgct tccacgaggc gacggcacag aatgcgccgg
    11161 acgtggtggt gtggggcagc ggtacaccga tgcgtgaatt cctgcacgtc gatgatatgg
    11221 cggcggcgag cattcatgtg atggagctgg cgcacgaagt ctggctggaa aacacccagc
    11281 cgatgctgtc gcacattaac gtcggcacgg gcgttgactg cactatccgc gagctggcgc
    11341 aaaccatcgc caaagtggtg ggttacaaag gtcgggtggt ttttgatgcc agtaaaccgg
    11401 atggtacgcc gcgcaaactg cttgatgtga cgcgcctgca tcagcttggc tggtatcacg
    11461 aaatctcact ggaagcgggg cttgccagca cttaccagtg gttccttgag aatcaagacc
    11521 gctttcgggg gtaatgatgt ttttacgtca ggaagacttt gccacggtag tgcgctccac
    11581 accgcttgtc tctctcgact ttattgtcga gaacagtcgc ggcgagtttc tgcttggcaa
    11641 aagaaccaac cgcccggcgc agggttactg gtttgtgccg ggagggcgcg tgcagaaaga
    11701 cgaaacgctg gaagccgcat tcgagcggct gacgatggcg gaactggggc tgcgtctgcc
    11761 gataacagca ggccagtttt acggtgtctg gcagcacttt tatgacgata acttctccgg
    11821 cacggatttc accactcact atgtggtgct cggttttcgc ttcagagtcg ctgaagaaga
    11881 tctgatactg ccggatgagc agcatgacga ttaccgctgg ctgacgccgg acgcgctgct
    11941 cgccagtaac gatgttcacg ctaacagccg cgcctatttt ctcgctgaga agcgtgccgg
    12001 agtacccgga ttatgaaaat actggtctac ggcattaact actcgccgga gttaaccggt
    12061 atcggcaaat acaccggcga gatggtggaa tggctggcgg cacaaggtca tgaggtgcgg
    12121 gttattaccg caccgcctta ctacccgcag tggcaggtgg gtgagaacta ttccgcctgg
    12181 cgctacaaac gagaagaggg ggccgccacg gtgtggcgct gcccgctgta tgtgccaaaa
    12241 cagccgagca ccctgaaacg cctgttgcat ctcggcagtt ttgccgtcag cagtttcttt
    12301 ccactgatgg cgcaacgtcg ctggaagccg gatcgcatta tcggcgtagt gccaacgctg
    12361 ttttgcacgc cgggaatgcg cctgctggcg aagctctctg gtgcgcgtac cgtgctgcat
    12421 attcaggatt acgaagtgga cgccatgctg gggctgggcc ttgccggaaa aggcaaaggc
    12481 ggcaaagtgg cacagctggc aacggcgttc gaacgtagcg gactgcataa cgtcgataac
    12541 gtttccacga tttcgcgttc gatgatgaat aaagccatcg aaaaaggcgt ggcggcggat
    12601 aacgtcatct tcttccccaa ctggtcggaa attgcccgtt ttcaacatgt tgcagacgcc
    12661 gatgttgatg cccttcgtaa ccagcttggc ctgccggata acaaaaaaat cattctttac
    12721 tccggcaata ttggtgaaaa gcaggggctg gaaaacgtta ttgaagctgc cgatcgtctg
    12781 cgcgatgaac cgctgatttt tgccattgtc gggcagggcg gcggcaaagc gcggctggaa
    12841 aaaatggcgc agcagcgtgg actgcgcaac atgcaatttt tcccgctgca atcgtatgac
    12901 gctttacccg cactgctgaa gatgggcgat tgccatctgg tggtgcaaaa acgcggcgcg
    12961 gcagatgccg tattgccgtc gaaactgacc aatattctgg cggtaggcgg taacgcggtg
    13021 attaccgcag aggcccacac agaactgggg cagctttgcg aaacctttcc gggcattgcg
    13081 gtttgcgtag aaccggaatc ggtcgaggcg ctggtggcgg ggatccgtca ggcgctcctg
    13141 ctgcccaaac acaacacggt ggcacgtgaa tatgccgaac gcacactcga taaagagaac
    13201 gtgttacgtc aatttataaa tgatattcgg ggataattat ggcgcagtcg aaactctatc
    13261 cagttgtgat ggcaggtggc tccggtagcc gcttatggcc gctttcccgc gtactttacc
    13321 ccaagcagtt tttatgcctg aaaggcgatc tcaccatgct gcaaaccacc atctgccgcc
    13381 tgaacggcgt ggagtgcgaa agcccggtgg tgatttgcaa tgagcagcac cgctttattg
    13441 tcgcggaaca gctgcgtcaa ctgaacaaac tcaccgagaa cattattctc gaaccggcag
    13501 ggcgtaacac tgcacctgcc attgcactgg cggcgctggc ggcaaaacgt catagcccgg
    13561 agagcgaccc gttaatgctg gtattggcgg cggatcatgt gattgccgat gaagacgcgt
    13621 tccgtgccgc cgtgcgtaat gccatgccat atgccgaagc gggcaagctg gtgaccttcg
    13681 gcattgtgcc ggatctacca gaaaccggtt atggctatat tcgtcgcggt gaagtgtctg
    13741 cgggtgagca ggatacggtg gcctttgaag tggcgcagtt tgtcgaaaaa ccgaatctgg
    13801 aaaccgccca ggcctatgtg gcaagcggcg aatattactg gaacagcggt atgttcctgt
    13861 tccgcgccgg acgctatctc gaagaactga aaaaatatcg cccggatatt ctcgatgcct
    13921 gtgaaaaagc gatgagcgcc gtcgatccgg atcttgattt tattcgtgtg gatgaagaag
    13981 cgtttctcgc ctgcccggaa gagtcggtgg attacgcggt catggaacgt acggcagatg
    14041 ctgttgtggt gccgatggat gcgggctgga gcgatgttgg ttcctggtct tcattatggg
    14101 agatcagcgc ccacaccgcc gagggcaacg tttgccacgg cgatgtgatt aatcacaaaa
    14161 ctgaaaacag ctatgtgtac gccgaatctg gcctggtcac caccgtcggg gtgaaagatc
    14221 tggtagtggt gcagaccaaa gatgcggtgc tgattgccga ccgtaacgcg gtgcaggatg
    14281 tgaaaaaagt ggtcgagcag atcaaagccg atggtcgcca tgagcatcgg gtgcatcgcg
    14341 aagtgtatcg tccgtggggc aaatatgact ctatcgacgc gggcgaccgc taccaggtga
    14401 aacgcatcac cgtgaaaccg ggcgagggct tgtcggtaca gatgcaccat caccgcgcgg
    14461 aacactgggt agtggtcgcg ggaacggcaa aagtcaccat tgatggtgat atcaaactgc
    14521 ttggtgaaaa cgagtccatt tatattccgc tgggggcgac gcactgcctg gaaaatccgg
    14581 ggaaaattcc gctcgattta attgaagtgc gctccggctc ttatctcgaa gaggatgatg
    14641 tggtgcgctt cgcggatcgc tacggacggg tgtaaacgtc gcatcaggcg ctgtttgtcg
    14701 gatgcggcgt gaacgcctta tccgacctac ggttcgggtt tgtagtcctg ataagacgcg
    14761 gcagcgtcgc atcaggcaat gaatgcgaaa ccgagatgta aataacgaca aaaacaaaat
    14821 tggccgtttc ggtcagggcc aactattgcc tgaaaaaggg taacgacatg aaaaaattaa
    14881 cctgctttaa agcctatgat attcgcggga aattaggcga agaactgaat gaagatattg
    14941 cctggcgcat tggtcgtgcc tatggcgaat ttctcaaacc gaaaaccatt gtgttaggcg
    15001 gtgatgtccg tctcaccagc gaaaccttaa aactggcgct ggcaaaaggt ttacaggatg
    15061 cgggcgtcga tgtgctggat atcggcatgt ccggcaccga agagatctat ttcgccacgt
    15121 tccatctcgg cgtggatggc ggcattgaag ttactgccag ccataatccg atggattaca
    15181 acggcatgaa actggtgcgc gaaggggctc gcccgatcag cggcgatacc ggactgcgcg
    15241 atgtccagcg tctggcagaa gccaacgact ttcctcccgt tgatgcaacc aaacgcggtc
    15301 gctatcagca aatcaatctg cgtgacgctt acgttgatca cctgttcggt tatatcaatg
    15361 tcaaaaacct cacgccgctc aagctggtga tcaactccgg gaacggcgca gcgggtccgg
    15421 tggtggacgc cattgaagcc cgctttaaag ccctcggcgc gcccgtggaa ttaatcaaag
    15481 tacacaacac accggacggc aatttcccca acggtattcc taacccgctg ctaccggaat
    15541 gtcgcgacga cacccgcaat gcggtcatca aacacggcgc ggatatgggc attgcctttg
    15601 atggcgattt tgaccgctgt ttcctgtttg acgaaaaagg gcagtttatc gagggctatt
    15661 acattgtcgg cctgctggca gaagcattcc tcgaaaaaaa tcccggcgcg aagatcatcc
    15721 acgatccacg tctctcctgg aacaccgttg atgtggtgac tgccgcaggt ggcacgccgg
    15781 taatgtcgaa aaccggacac gcctttatta aagaacgtat gcgcaaggag gacgccatct
    15841 acggtggcga aatgagcgct caccattact tccgtgattt cgcttactgc gacagcggca
    15901 tgatcccgtg gctactggta gccgaactgg tgtgcctgaa agagaaaacg ctgggcgaac
    15961 tggtacgcga ccggatggtg gcgtttccgg caagcggtga gatcaacagc aaactggcgc
    16021 aacccgctga ggcgattaac cgcgtggaac agcattttag ccgtgaggcg ctggcggtgg
    16081 atcgtaccga tggcatcagt atgacctttg ccgactggcg ttttaacctg cgctcctcca
    16141 acaccgaacc ggtggtgcgg ttgaatgtgg aatcgcgcgg tgatgtgccg ctgatggaag
    16201 cgcgaacgcg aactctgctg acgttgctga acgagtaatg tcggatcttc ccttacccca
    16261 ctgcgggtaa ggggctaata acaggaacaa cgatgacaaa tctaaaaaag cgcgagcgag
    16321 cgaaaaccaa tgcatcgtta atctctatgg tgcaacgctt ttcagatatc accatcatgt
    16381 ttgccggact atggctggtt tgcgaagtga gcggcctgtc attcctctac atgcacctgt
    16441 tggtggcact gattacgctg gtggtgttcc agatgctggg cggcatcacc gatttttatc
    16501 gctcatggcg cggtgttcgg gcagcgacag aatttgctct gttgctacaa aactggacgt
    16561 taagcgtgat tttcagcgcc ggactggtgg cgttcaacaa tgatttcgac acgcaactga
    16621 aaatctggct ggcgtggtat gggctgacca gcatcggact ggtggtttgc cgttcatgta
    16681 ttcgtattgg ggcgggctgg ctgcgtaatc atggctataa caagcgcatg gtcgccgtag
    16741 cgggggattt agccgccgga caaatgctga tggagagctt ccgtaatcag ccgtggttag
    16801 ggtttgaagt ggtgggggtt taccacgacc cgaaaccggg cggcgtttct aacgactggg
    16861 cgggtaacct gcaacagctg gtcgaggatg ctaaagcggg taagattcat aacgtctata
    16921 tcgcgatgca aatgtgcgac ggcgcgcgag tgaaaaaact ggtccatcaa ctggcggaca
    16981 ccacctgttc ggtgctgctg atccccgatg tctttacctt caacattctc cattcacgtc
    17041 tcgaagagat gaacggcgtt ccggtggtgc cgctgtatga cacgccgctt tccggggtta
    17101 accgcctgct aaaacgtgcg gaagacattg tgctggcgac gctgatcttg ctgctgatct
    17161 ccccggtgct gtgctgtatt gcgctggcgg tgaaactcag ttcacctggg ccggttattt
    17221 tccgccagac tcgctatggc atggatggca agccgatcaa agtgtggaag ttccgttcca
    17281 tgaaagtgat ggagaacgac aaagtggtga ctcaggcgac gcagaacgat ccgcgcgtca
    17341 ccaaagtggg gaactttctg cgccgcacct cgctggatga actgccgcag tttatcaatg
    17401 tgctgaccgg ggggatgtcg attgtcggtc cacgtccgca cgcagtagcg cataacgaac
    17461 agtatcgcca gctcattgaa ggctacatgc tgcgccacaa agtgaaaccg ggcattaccg
    17521 gctgggcgca gattaatggc tggcgcggcg aaaccgacac gctggagaaa atggaaaaac
    17581 gcgtcgagtt cgaccttgag tacatccgcg aatggagcgt ctggttcgat atcaaaatcg
    17641 ttttcctgac ggtgttcaaa ggtttcgtta acaaagcggc atattgatat gagcttacgt
    17701 gaaaaaacca tcagcggcgc gaagtggtcg gcgattgcca cggtgatcat catcggcctc
    17761 gggctggtgc agatgaccgt gctggcgcgg attatcgaca accaccagtt cggcctgctg
    17821 actgtgtcgc tggtgattat cgcgctggca gatacgcttt ctgacttcgg tatcgctaac
    17881 tcgattattc agcgaaaaga aatcagtcac cttgaactca ccacgttgta ctggctgaac
    17941 gtcgggctgg ggatcgtggt gtgcgtggcg gtgtttttgt tgagtgatct catcggcgac
    18001 gtgctgaata acccggacct ggcaccgttg attaaaacat tgtcgctggc gtttgtggtg
    18061 atcccccacg ggcaacagtt ccgcgcgttg atgcagaagg agctggagtt caacaaaatc
    18121 ggcatgatcg aaaccagcgc ggtgctggcg ggcttcactt ttacggtggt tagcgcccat
    18181 ttctggccgc tggcgatgac cgcgatcctc ggttatctgg tcaatagtgc ggtaagaacg
    18241 ctgctgtttg gctactttgg tcgaaaaatt tatcgccccg gtctgcattt ctcgctggcg
    18301 tcagttgcgc cgaacttacg ctttggtgcc tggctgacgg cggacagcat catcaactat
    18361 ctcaacacca acctttcaac gctggtgctg gcgcgtattc ttggcgcggg cgtggcaggg
    18421 ggatacaacc tggcgtacaa cgtggccgtc gtaccgccga tgaagctgaa cccaatcatc
    18481 acccgcgtgt tatttccggc attcgccaaa attcaggacg acaccgaaaa gttgcgcgtg
    18541 aacttctaca agctgctgtc ggtggtgggg attatcaact ttccggcgct gctcgggctg
    18601 atggtggtgt cgaataactt tgtaccgctg gtctttggtg agaagtggaa cagcattatt
    18661 ccggtgctgc aattgctgtg tattgtgggt ctgctgcgct ccgtgggtaa cccgattggt
    18721 tcgctgctga tggcgaaagc gcgggtcgat atcagcttta aattcaacgt attcaaaaca
    18781 tttctgttta ttccggcgat tgttattggt gggcagatgg cgggcgcaat cggcgtcacg
    18841 ctcggtttcc tgctggtgca gattatcaac accattctga gttacttcgt gatgattaaa
    18901 ccggtactcg gttccagtta tcgccagtac atcctgagtt tgtggctgcc gttttatctc
    18961 tcgctgccga cgctggtggt cagttatgcg ctgggcattg tgctgaaagg gcaactggcg
    19021 ctggggatgc tgctggcggt gcaaatagcc gcgggcgtgc tggcgtttgt ggtgatgatt
    19081 gtgctgtcgc gccatccgct gatggtggaa gtgaagcgtc agttttgtcg cagcgaaaaa
    19141 atgaaaatgc ttttacgggc ggggtgagtt gctgttttcg gttccgacaa cggttgccgg
    19201 gtgcagcgta aaacgcctta tccatcctac ggttctgtgc tcgggacctg taggcctgat
    19261 aagacgcgcc agcgtcgcat caggcaaaaa gcggcaacga acacaacaaa cacagcctca
    19321 cctggcatca aatttatgag gaaaaaatga aattacttat tctgggcaac cacacttgcg
    19381 gcaatcgtgg cgacagtgcc atcttgcgcg gcttacttga tgccatcaac atcctcaatc
    19441 cacaagccga agtggacgtg atgagccgct atccggtcag ttcttcctgg ctgctcaacc
    19501 gcccggtaat gggcgatccg ctgttcctgc aaatgaaaca acacaacagc gcggcgggcg
    19561 ttgtcgggcg cgttaaaaaa gtcctccgtc gccgctatca acaccaggta ttgctctcac
    19621 gcgtcaccga cactggcaag ctgcgtaata tcgccatcgc ccagggattc accgacttcg
    19681 tgcgcatgct ctcaggttac gacgccatta ttcaggtcgg aggatcgttt tttgtcgatc
    19741 tctacggcgt gccgcagttt gaacatgcgc tttgcacgtt tatggcgaaa aagccgctgt
    19801 ttatgattgg tcacagcgtc ggccccttcc aggacgagca atttaaccaa ctggcgaact
    19861 acgtttttgg tcactgtgac gcgctgatcc tgcgcgaatc ggtcagcctt gatttgatga
    19921 aacgcagcaa tatcaccact gcaaaagttg aacatggcgt cgataccgcg tggctggtcg
    19981 atcaccacac agaagatttc accgccagct atgccgtcca acactggctg gacgttgccg
    20041 cacaacagaa aacggtggca attaccctgc gcgaactggc accgtttgac aaacgcctcg
    20101 gcaccactca acaagcgtat gaaaaagcct ttgccggggt ggtcaatcgc attctcgacg
    20161 gaggctatca ggtcattgcg ctctccacct gtacgggcat cgacagctat aacaaagacg
    20221 accgcatggt agcgctcaat ctgcgccagc acatcagcga tcctgcccgt taccacgtag
    20281 tgatggatga actcaacgat ctggaaatgg gcaaaattct cggggcctgt gaactcaccg
    20341 tcggtacgcg cctgcactcc gccattatct cgatgaattt tgccactccg gcaattgcca
    20401 tcaactacga acataaatcc gccgggatca tgcaacaact ggggctgcca gagatggcga
    20461 ttgatatccg tcatttactg gacggcagct tgcaagcgat ggttgcggat accttaggcc
    20521 agcttccggc gctgaacgca cgacttaacg aagccgtcag tcgcgaacgt cagacaggaa
    20581 tgcagatggt gcagtctgtg cttgagcgca tcggggaggc gaaatgaagg tcggcttctt
    20641 tttactgaaa tttccgctgt cgtcggaaac tttcgtcctc aaccaaatta ccgcgtttat
    20701 tgatatggga tttgaggtgg agattgtcgc gctgcaaaaa ggcgatacag agaacaccca
    20761 cgcggcatgg acgaaataca accttgccgc cagaacccgc tggttacagg acgaaccaac
    20821 gggcaaagtg gcgaagctgc gccaccgcgc cagccagacg ttacgcggca ttcatcgtaa
    20881 aaatacctgg caggcgctca acctcaaacg ctatggtgcc gagtcgcgga acctgatttt
    20941 gtctgccatt tgcggtcagg tcgcaacacc atttcatgcc gatgtgttca tcgctcattt
    21001 tggcccggcg ggggtaaccg cagcaaaact acgggaactg ggggtcattc gcggcaaaat
    21061 tgccactatc ttccacggta ttgatatctc cagtcgggaa gtgctcaacc actacactcc
    21121 cgaatatcag caactgttcc gccgtggcga cctgatgcta ccgataagtg atttgtgggc
    21181 cggaaggctg caaaaaatgg gctgcccgag ggaaaaaatt gccgtatcgc gcatgggcgt
    21241 ggacatgacg cgctttagcc cgcgtccggt gaaagcgccc gcaacgccgc tggaaatcat
    21301 atccgtcgca cgcttaaccg agaaaaaagg tctgcatgta gcgattgaag cctgccgtca
    21361 gttgaaagag cagggcgtgg catttcgcta tcgcatcctc ggcattggcc cgtgggaacg
    21421 acgcctgcgt accctcatcg aacaatatca actggaagat gtggtagata tgccgggctt
    21481 taaaccgagc cacgaagtga aagcgatgct cgacgacgcg gatgtcttcc tgttgccatc
    21541 ggtaacgggc gcggatggcg atatggaagg cattccggtg gcgctaatgg aagcgatggc
    21601 ggtcggcatt ccggtggttt ctactctgca tagcggaata ccggaactgg tggaggccga
    21661 taaatccggc tggctggtgc ctgagaacga tgctcgcgca ctggcgcaac gactggcggc
    21721 gtttagccaa ctggacaccg acgaactggc tccggtcgtc aaacgtgctc gcgaaaaagt
    21781 cgaacacgat tttaaccagc aggtgattaa tcgggaactc gccagcttgt tacaggcttt
    21841 atagaggaga acgcatgcca tttaaaaaac tctcccgacg caccttcctg acggcaagct
    21901 cggcgcttgc cttcctccat acccctttcg ctcgcgcact tcccgcccga caaagcgtta
    21961 acattaacaa ctacaaccct cacgactgga tcgcctcatt taaacaagcc ttcagcgaag
    22021 ggcaaacggt cgtcgtgcct gctggattgg tttgtgacaa tatcaacacc ggcatcttca
    22081 ttcctgcagg taaaacgtta cacatccttg gaagcctgcg cggcaacggc agagggcgat
    22141 ttgtcttaca ggacggcagc caggtgacag ggggggaggg cggcagtatg cataacatca
    22201 ccctggatgt gcgcgggtct gactgcacca ttaaagggct ggcgatgagc ggctttggcc
    22261 cggtaacgca gatttatatc ggcggcaaaa acaaacgggt catgcgtaac ctgaccatcg
    22321 ataacctgaa cgttagccac gctaattacg ccatcttacg ccagggattt cataaccaga
    22381 ttatcggtgc caacatcacc aattgtaagt tcagtgattt acagggggac gccatcgaat
    22441 ggaacgtggc aattaacgac agtaatattt tgatttccga ccatgtcatt gagcgcatca
    22501 actgtactaa cggaaaaatc aactggggca tcggcatagg ccttgcgggg agtacttatg
    22561 ataataacta cccggaagac caggcagtga aaaactttgt cgtggcgaat atcacgggat
    22621 cggattgtcg gcagttgata catgttgaaa atggtaaaca ttttgttatt cgtaatatca
    22681 aagcccgcaa tatcacaccg gatttcagta agaaagcagg tattgataac gccacggtcg
    22741 ctatttacgg ttgtgacaat ttcgtgattg ataatattga aatgattaat agcgccggga
    22801 tgttaatcgg ctatggggta attaaaggca aatatctctc gataccgcaa aatttccgag
    22861 tgaataatat tcaactggat aatacccacc ttgcttacaa attgcgcggt atccaaatct
    22921 ccgccggcaa tgccgtctcc tttgtggcac tgactaacat tgagatgaag cgtgcctcgc
    22981 ttgagctaca caataaaccg caacaccttt ttatgcgaaa tatcaaggtg atgcaggaat
    23041 cctcagttgg acccgcattg agcatgaact tcgacatgcg caaagacgtt cgaggcgtct
    23101 ttatggcgaa aaaagaaaca ctgctgtcac ttgcaaatgt tcatgcggtg aatgaaaaag
    23161 gacaaagctc cgtcgatatc gacaggataa atcaccatat tgttaatgtg gaaaagatta
    23221 actttagatt gccggaacgg agggagtaga tttgcgacca ttcctggaaa aatggagcca
    23281 tacttaggaa caatgctaca gcaatccaca acgaagcggc gtaacatcac aagtaattca
    23341 gtaatcaatt cagggtaatt gatgctggcg aaaaaaatcg aacaagctat aattcagcaa
    23401 ccattttaca ggtggatgaa ataatgacga atttaaaagc agttattcct gtagcgggtc
    23461 ttgggatgca tatgttgcct gccactaagg cgattcccaa agagatgcta ccgatcgtcg
    23521 acaagccaat gattcagtac attgttgacg agattgtggc tgcagggatc aaagaaatcc
    23581 tcctggtaac tcacgcgtcc aagaacgcgg tcgaaaacca cttcgacacc tcttatgaat
    23641 tagaatctct ccttgagcag cgcgtgaagc gtcaactgct ggcggaagtg cagtccatct
    23701 gtccgccggg cgtgaccatt atgaacgtac gtcagggcga acctttaggt ttaggccact
    23761 ccattttatg tgcacgacct gccattggtg acaatccatt tgtcgtggta ctgccagacg
    23821 ttgtgatcga cgacgccagc gccgacccgc tgcgctacaa ccttgctgcc atgattgcac
    23881 gattcaacga aacgggtcgt agccaggtgc tggcaaaacg tatgccgggt gacctctctg
    23941 aatactccgt cattcagacc aaagaaccac tggatcgtga aggtaaagtc agccgcattg
    24001 ttgaattcat cgaaaaaccg gatcagccgc agacgctgga ctcagacatc atggccgttg
    24061 gtcgctatgt gctttctgcc gatatttggc cggaacttga acgcactcag cctggtgcat
    24121 ggggacgtat tcagctgact gatgccattg ctgaactggc gaaaaaacag tccgttgatg
    24181 ccatgctgat gacaggtgac agctacgact gcggtaaaaa aatgggttat atgcaggcat
    24241 ttgtgaagta tggactacgc aacctaaaag aaggggcgaa gttccgtaaa gggattgaga
    24301 agctgttaag cgaataatga aaatctgacc ggatgtaacg gttgataaga aaattataac
    24361 ggcagtgaag attcgtggcg aaagtaatat gttgcgaata ttcctgccgt tgttttatat
    24421 aaacaatcag aataacaacg agttagcaat aggattttag tcaaagtttt ccaggatttt
    24481 ccttgtttcc tgagcggatt ggtaagacaa ttagcgtttg aatttttcga gttaagcgcg
    24541 agtgggtaac gctcgtcaca tcgtaggcat gcatgcagtg ctctggtagc tgtaaagcca
    24601 ggggcggtag cgtat
//
LOCUS       DAPGOF010000066        24237 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.066, whole genome shotgun sequence.
ACCESSION   DAPGOF010000066 DAPGOF010000000
VERSION     DAPGOF010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24237
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.066"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..557
                     /locus_tag="RHV13_002283"
     CDS             <1..557
                     /locus_tag="RHV13_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HDV1241812.1"
                     /translation="SDLKETTSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPS
                     TQVGQKVLDKILNYTNTEMKEAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYE
                     VVPVALVVAGTQMATGHRTMDTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPM
                     AFENIKQVIDDMATDATMFDVNKK"
     gene            complement(575..1081)
                     /gene="tnpA"
                     /locus_tag="RHV13_002284"
                     /pseudo
     CDS             complement(575..1081)
                     /gene="tnpA"
                     /locus_tag="RHV13_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            1089..2297
                     /locus_tag="RHV13_002285"
     CDS             1089..2297
                     /locus_tag="RHV13_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV1241813.1"
                     /translation="MKRLQAFKFQLRPGGQQKCEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTAVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRC
                     AYCGHTAKENRLSQSKFRCHVCGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQ
                     EPTEMIQATA"
     gene            complement(2337..3551)
                     /gene="ydcO"
                     /locus_tag="RHV13_002286"
     CDS             complement(2337..3551)
                     /gene="ydcO"
                     /locus_tag="RHV13_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HDV1241814.1"
                     /translation="MLRCLYAITHEVTMRLFSVPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGASTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDCQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLLAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNRY"
     gene            3604..4140
                     /gene="sutR"
                     /locus_tag="RHV13_002287"
     CDS             3604..4140
                     /gene="sutR"
                     /locus_tag="RHV13_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HDV1241815.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQSLNCGEGARFAADVTHIYR
                     NGGEQIVHFHSLIHYPRS"
     gene            4213..6174
                     /gene="rlhA"
                     /locus_tag="RHV13_002288"
     CDS             4213..6174
                     /gene="rlhA"
                     /locus_tag="RHV13_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HDV1241816.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLEQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVLIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTIYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(6266..6496)
                     /locus_tag="RHV13_002289"
     CDS             complement(6266..6496)
                     /locus_tag="RHV13_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HDV1241817.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            6718..6894
                     /gene="hicA"
                     /locus_tag="RHV13_002290"
     CDS             6718..6894
                     /gene="hicA"
                     /locus_tag="RHV13_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HDV1241818.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKVRFHGMRSVMPRHPCDEIK
                     EPLRKAIQKQLGLS"
     gene            6940..7356
                     /gene="hicB"
                     /locus_tag="RHV13_002291"
     CDS             6940..7356
                     /gene="hicB"
                     /locus_tag="RHV13_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HDV1241819.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            7435..8841
                     /locus_tag="RHV13_002292"
     CDS             7435..8841
                     /locus_tag="RHV13_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDV1241820.1"
                     /translation="MKKYQQLAEQLREQIASGIWQSGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGHIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQEYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVAL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            9086..10231
                     /locus_tag="RHV13_002293"
     CDS             9086..10231
                     /locus_tag="RHV13_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDV1241821.1"
                     /translation="MSKTFARSSLCALSMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALITNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLQDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            10249..11262
                     /locus_tag="RHV13_002294"
     CDS             10249..11262
                     /locus_tag="RHV13_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV1241822.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            11263..12204
                     /locus_tag="RHV13_002295"
     CDS             11263..12204
                     /locus_tag="RHV13_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV1241823.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            12194..12988
                     /locus_tag="RHV13_002296"
     CDS             12194..12988
                     /locus_tag="RHV13_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV1241824.1"
                     /translation="MHSERAPFLLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFGVAAQRSDILDSVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            13010..14434
                     /gene="patD"
                     /locus_tag="RHV13_002297"
     CDS             13010..14434
                     /gene="patD"
                     /locus_tag="RHV13_002297"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HDV1241825.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTAPSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(14531..14626)
                     /gene="yncL"
                     /locus_tag="RHV13_002298"
     CDS             complement(14531..14626)
                     /gene="yncL"
                     /locus_tag="RHV13_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HDV1241826.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            14821..14994
                     /gene="ortT"
                     /locus_tag="RHV13_002299"
     CDS             14821..14994
                     /gene="ortT"
                     /locus_tag="RHV13_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HDV1241827.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            15080..15313
                     /locus_tag="RHV13_002300"
     CDS             15080..15313
                     /locus_tag="RHV13_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HDV1241828.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(15314..15763)
                     /locus_tag="RHV13_002301"
     CDS             complement(15314..15763)
                     /locus_tag="RHV13_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDV1241829.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(15760..16278)
                     /gene="mddA"
                     /locus_tag="RHV13_002302"
     CDS             complement(15760..16278)
                     /gene="mddA"
                     /locus_tag="RHV13_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HDV1241830.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            16459..17496
                     /gene="curA"
                     /locus_tag="RHV13_002303"
     CDS             16459..17496
                     /gene="curA"
                     /locus_tag="RHV13_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HDV1241831.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            17694..18359
                     /gene="mcbR"
                     /locus_tag="RHV13_002304"
     CDS             17694..18359
                     /gene="mcbR"
                     /locus_tag="RHV13_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HDV1241832.1"
                     /translation="MPGMGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALTVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(18395..20497)
                     /locus_tag="RHV13_002305"
     CDS             complement(18395..20497)
                     /locus_tag="RHV13_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDV1241833.1"
                     /translation="MKIFSVRQTVLPALLALSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTSVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDDASKVSLIFNSVDIKADDPGGLTEAEWKANPQHAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            20739..21800
                     /locus_tag="RHV13_002306"
     CDS             20739..21800
                     /locus_tag="RHV13_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HDV1241834.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(21913..23412)
                     /gene="ansP"
                     /locus_tag="RHV13_002307"
     CDS             complement(21913..23412)
                     /gene="ansP"
                     /locus_tag="RHV13_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HDV1241835.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGDVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVIFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIELVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     LLLPWNAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IASLPIIGLLLVVGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            23679..>24237
                     /locus_tag="RHV13_002308"
     CDS             23679..>24237
                     /locus_tag="RHV13_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HDV1241836.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFFDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQLFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNATNISAIA"
BASE COUNT         6179 a         5956 c         6268 g         5834 t
ORIGIN      
        1 actctgattt aaaagaaaca acctcggcta caggtaaacc ggttttacgt tgggtagacc
       61 cgagttttga tcaaagcaaa tatgacagca tcgtctggaa cccaatcact tattatccgg
      121 taccgaaacc ttcgacccag gtagggcaga aagttctgga taaaattttg aactatacca
      181 acaccgaaat gaaagaagcg atagcgcagc gtaaaccact ggttaccacc gctgggccgc
      241 gtagtctgat tttccgtggg gccattaccg gtgtagatac cagcaaagaa gggctgcaat
      301 tctatgaagt ggttcctgtt gcattagtgg ttgcggggac gcaaatggct acaggccacc
      361 gtaccatgga tactcgcctc tattttgaag gtgagctgat tgatgcagcg actaataaac
      421 cggttatcaa agtcgttcgt cagggcgaag gtaaagacct gaataacgaa agtacgccaa
      481 tggctttcga aaatattaaa caagttattg atgacatggc gaccgatgcc accatgtttg
      541 acgttaacaa aaagtagttc cagacgcgcc atcgttagat ggcgcttttt atccggtgcg
      601 ccgtaaaacc ccatccttca gggcggggat ataaggcgcg gttttccacc tgaccaggtg
      661 tttgctgttg ttcaatgtat tggcggatga cggatattgg cgcacctccg cagctactgg
      721 caaaatagcc aggactccac aaaacgcctt tgtagtaata cctgactgca atatctggtc
      781 gatctcgtcg cagtaaccta cccgatacgc ctttgaggct gtttaccaga ctggatatcg
      841 ccagtttggg aggttagtta attaacaaat ggacgtgatc tggttcgcca tccatttcaa
      901 ccagttcagc ttcaaaataa gcacatacat ttgaaaagta agtgtgtagt ttttctgtcg
      961 cgtcatggtc aaaaatctgg cgtcggtatc tggtgacaaa gaccaggtga acatgcatca
     1021 ggaaaacaca atgccttcca cgccggatat cggtttcttt tttcacagac caaagtatca
     1081 tttgacctgt gaaacgatta caggcattta aattccagtt aagaccaggt ggtcaacaga
     1141 agtgtgaaat gaggcgcttc gcaggcgcat gtcgtttcgt tttcaatcgt gctctggcac
     1201 gtcagaatga gaatcatgag gccggtaata aatacatccc ttacgggaaa atggcttcct
     1261 ggctggttga gtggaaaaat gccactgaaa cgcaatggct taaagattct ccctcacagc
     1321 cattgcaaca gtcactgaaa gaccttgagc gggcttacaa aaacttcttc cggaagcggg
     1381 ctgcttttcc ccgattcaaa aagcggggac agaatgatgc attccgctac ccgcagggcg
     1441 ttaagctcga tcaggaaaac agccgtattt ttctgccgaa actgggctgg atgcgctacc
     1501 ggaatagccg tcaggtcacg ggtgttgtga aaaatgtcac tgtcagccag tcctgcggta
     1561 agtggtacat cagtattcag acagaaagtg aagtatcaac tccggttcac ccttcagcat
     1621 caatgatcgg gctggatgct ggcgtggcta aactcgccac gctgtcagat ggcacagtct
     1681 ttgagcctgt aaacagtttt cagaaaaacc agaagacgct ggcgagactt cagcgacagt
     1741 taagccgcaa ggtcaaattc agcaacaact ggcagaagca gaaacgcaaa atacagcgac
     1801 tgcattcctg tatcgcaaat atccgcaggg actaccttca caaagtcaca acggccgtca
     1861 gcaaaaacca cgcaatgata gtcattgagg atttgaaggt cagcaacatg tcaaagtcag
     1921 cagcgggtac ggtcagccag ccggggcgca atgtccgggc aaaatcaggt ttaaaccgtt
     1981 cgatactgga tcagggctgg tatgaaatgc gccgccagct tgagtacaag cagctctggc
     2041 gtggcggtca ggtgcttgct gttccgccag cgtatacaag ccagcgttgc gcgtactgtg
     2101 gtcatacagc gaaagagaac cgcctgtcac aaagtaaatt cagatgccat gtatgtggat
     2161 atacagcgaa cgccgatgta aatggcgctc gtaacatttt agcggcgggg cacgccgttc
     2221 ttgcctgtgg agagatggtg cagtcaggcc gcccgttgaa gcaggaaccc accgaaatga
     2281 ttcaggcgac agcctgaacg tagcagggat ccacgtcctt cagggcgtgg aggatgtcaa
     2341 tatcggtttc tgtcagcgat taaattcaac accacgtaac aaacgcctcc ggcaattaat
     2401 ccccagaacg ctgaaccaat cccgaccagc gttaatccac ttgccgttac cagaaatgcc
     2461 accaccgccg cgtcgcgctc acgctcatta tgcagcgcct gatacaaact gccgccaatg
     2521 gtacttaaca gcgccagacc tgccagcatc tggatccaac ttacgggcag ggcagccatc
     2581 atcccggtaa tggcactacc aaacagacct gcgagcaaat agaaaatgcc tgcaacggca
     2641 gcggccagcc aacgttgatc tttatccgga tgcgcttccg ggctttggca aatagctgcg
     2701 gtgattgccg caataccgac ggaataaacg ccgaaagggg aaaaaaccag tgccagcaat
     2761 ccagtaaata caattaatgg cgaaacagga gccgaatatc cggctgcttt cattgctgcg
     2821 atacccggtg cgttttgcga tgccatcgtc accagaaaaa gggggagtgc aacgctcagg
     2881 ctgtgagcaa acgaaaaatc aggggtaata taagtgggga gaacgggttt aaagacaaca
     2941 tcagttgtga caacgtcacc ttgcgcgata acgatcacga tcccaataat catcgcggca
     3001 attaccgcat agcgcggcgc aacggccttg gttgccagcc ataccagcaa catacttcca
     3061 cacaacgtaa attgacagtc cagactggca aacgcctgta aaccaaagcg taataaaatc
     3121 ccggcaagca ttgccgccgc aagcgagtgc ggaataatgc gcatcagacg agcaaagagt
     3181 cccgttatgc cgcagaggac tattaacgcg ttggtgacaa taaaaacgcc gatggcttcg
     3241 ttaagtgtta gtccctgcaa tccggtgacc aacaaagccg cgccaggcgt tgaccatgcg
     3301 gtgagaacag gtacgcgata ccataatgtc agagtcagcg tactgacgcc cattgccagc
     3361 cccagcgccg tcatccagcc agagatttgt gcagtggagg ctccggcgac aatcgctgct
     3421 tgccagatta ttgccgctga actggcgtag ccaattaata ccgccagaaa ccccgccagt
     3481 agcgtgggtg gaggaacaga aaacagacgc atagtcacct cgtgcgttat agcgtacagg
     3541 caccgtagca tttgtccgtt ataacgcaca agtgataaac ttccgttttg ccggaggagt
     3601 cgcatggaaa atctcgctcg ctttttatcc accacactta aacaactacg tcagcagcgc
     3661 ggctggagtc tttcgcgact ggcagaagcg acgggcgttt ctaaagcaat gttaggacag
     3721 attgagcgta atgagtccag cccgacggtc gcgacattat ggaaaattgc cactgggttg
     3781 aacgtgccct tttcgacatt tatttctccg ccacagtccg ctacgccttc agtttatgat
     3841 ccacaacagc aggcgatggt gatcacttcg ctgtttcctt atgatccaca actctgcttt
     3901 gaacacttct cgattcagat ggcacccggt gcaataagcg aatcgacgcc ccatgagaag
     3961 ggggtaatcg aacatgtcgt cgtcattgat ggacaacttg atctgtgcgt tgatggtgaa
     4021 tggcagagcc ttaattgtgg ggaaggcgct cgatttgctg cagacgtcac gcatatctat
     4081 cgtaatggtg gggagcaaat cgtacatttt cattccctca tccattaccc gcgcagttaa
     4141 gcgggaaaac tatttcgcaa cgtcgcgctt gtggctaaaa tagccgccat ttttcagcta
     4201 ctggataaga atgtgaccgt atcttctcat cgacttgaac tgttaagccc ggcacgcgat
     4261 gccgccattg cccgcgaagc tattttgcac ggtgccgatg ctgtttatat cggcggccct
     4321 ggttttggtg cccgtcataa tgccagtaat agcctgaaag atattgccga gctggtgccg
     4381 tttgcccatc gttatggtgc aaaaattttc gtcacgctta acaccatttt gcatgatgat
     4441 gagctggaac ccgcgcaacg gctgattact gacctctacc agaccggtgt cgatgcgctg
     4501 attgttcagg atatggggat tctggaactt gatattccgc cgattgaact gcacgccagt
     4561 acgcagtgcg acattcgtac agtagaaaaa gcgaagttcc tctctgatgt tggcttcacg
     4621 cagattgtgc tggcgcgaga gctgaatctt gagcagatcc gcgcgattca ccaggccacg
     4681 gacgcgacca ttgaattctt tattcatggg gcactgtgcg tggcctattc gggtcagtgc
     4741 tacatttctc atgcgcaaac agggcgtagc gccaaccgtg gcgattgctc gcaggcgtgc
     4801 cgtttgccat acacattgaa agacgatcag gggcgggtgg tttcctatga aaaacatctg
     4861 ctgtcgatga aagataacga tcaaactgcc aacctcggcg cgctgattga tgctggtgta
     4921 cgctccttca agattgaagg gcgttacaaa gatatgagct acgtgaagaa tatcaccgcc
     4981 cattatcgcc agatgctgga tgccattatt gaagaacgtg gcgatctgac gcgcgcttca
     5041 tcaggtcgta ctgaacattt ctttgttcca tcgacggaaa agactttcca tcgtggtagc
     5101 acagattatt ttgtgaatgc ccgtaaaggc gatattggcg cgttcgattc gccgaaattt
     5161 atcggcctgc cggtgggtga agtattgaaa gtggcgaaag atcatctcga tgttgccgtt
     5221 accgagccac tggcaaatgg cgatggcctg aacgtgttga ttaaacgtga agtcgtcggt
     5281 tttcgtgcca atacggtcga gaaaaccgga gaaaatcagt accgcgtctg gcccaatgaa
     5341 atgccagcag atttgcacaa aattcgcccg catcacccac taaaccgtaa tcttgatcat
     5401 aactggcagc aggcactgac aaaaacctcc agcgaacgtc gggtggcggt agacattgaa
     5461 ctgggcggct ggcaggaaca actgattctg accctcacca gtgaagaggg ggtcagcatc
     5521 acgcatacgc tggatgggca gttcgacgaa gccaataacg ccgaaaaagc aatgaacaat
     5581 ctgaaggatg gtctggcaaa actggggcaa accatctatt acgcccgcga tgtgcaaatt
     5641 aatttgccgg gggcgctgtt tgtaccaaac agtctgttaa accagttccg ccgcgaagcc
     5701 gccgacatgc tggatgctgc acgtcttgcc agttaccagc gcggcagccg taaaccggtt
     5761 gctgatcctg cgccggttta tccgcaaacg catctgagtt tcctcgcgaa cgtttacaac
     5821 cagaaagcgc gtgaatttta tcatcgctat ggtgtgcagc tgattgacgc ggcgtatgag
     5881 gcacatgaag agaagggcga agtcccggtg atgatcacca agcattgtct gcgctttgcc
     5941 tttaatctgt gcccgaaaca ggcgaaaggc aatatcaaaa gctggaaggc gacgccaatg
     6001 caactggtta acggcgatga agtattaacg ctaaagtttg attgccgccc atgcgagatg
     6061 cacgtcattg gcaaaatcaa aaatcacata ctgaaaatgc cgttaccggg aagcgtagtg
     6121 gcatccgtaa gtccggatga gctgctgaaa acattgccga agcgaaaagg gtaaaacgcc
     6181 agttttctgg ttactcacaa cttattgaat ctgcatgata ttgcctgccg ggtaaggcgt
     6241 tacgccgcat ccggcatcaa acgactcaac gttgtcccgc ttctggtttg cgcgattttt
     6301 gccagtaaaa atgctcgcgc aaaccttccg ccgactcttc cgccacagca cgcaattcat
     6361 cgctgtccgc ttcatgacgc agctgatgat ccacattctt tacccacaca aattcatgtc
     6421 ctttgtgccc tgccatgagt tgtcctgaga acagcgcaca cgttaataag acaaccgata
     6481 acgccttcgt aaacatcctg ccaccttttt tttacctttt gccgctatga tgccgatcgt
     6541 ttcttgaggt tattattcag ttttgcaaat tagcgcaaag aaattctgga atcttccttc
     6601 ctgattttgc attgcattct gccgttgcgg caatttagtg ctattttcga gcaaattaca
     6661 cacggaggta aacactaatg tttactttgt tgatatactc agcggcaggg aggcgatgtg
     6721 aaacaaagcg agttcagacg ttggctcgaa tctcagggcg tcgatgtagc gaatggcagc
     6781 aaccatttga aagtcaggtt tcatgggatg cgcagtgtca tgccgcgtca cccctgcgat
     6841 gagattaaag aaccattgcg taaagcaatc cagaaacaac tcggtttgag ttaatcgcca
     6901 attaaaaagg ttaatgacat gcgagagaca gtcgaaatta tgcgttatcc cgtcactctt
     6961 acacccgcgc cggaaggcgg ttatatggtt tcttttgtgg atatccctga agcgttgacc
     7021 cagggcgaaa ctgtcgctga agcgatggaa gcggcaaaag atgctttact gaccgcattt
     7081 gatttttatt ttgaagataa cgagcttatc ccgttacctt cgccattaaa tagtcacgat
     7141 cattttattg aagtaccttt gagcgtcgcc tctaaggtat tgctgttaaa tgctttttta
     7201 cagtcagaaa tcactcagca agagttagcc aggagaattg gcaaacctaa acaggaaatt
     7261 actcgtttat ttaacttgca ccatgcgaca aaaatcgacg ccgtccagct cgcggctaag
     7321 gcgcttggca aagagttatc actggtgatg gtttgataac agttaatgaa aagttgtcat
     7381 ttttaacaac tgatatagac tgccgaatca tctgcacata attacgattc gataatgaaa
     7441 aaatatcagc agcttgcaga acaattacgc gagcagattg cgtctggtat ctggcaatcc
     7501 ggcgatcgtt tgccttcgtt gcgtgaccag gtggcgcttt ctggcatgag ctttatgact
     7561 gtcagccatg cctatcagtt gctcgaaagt cagggacata ttatcgcgcg accgcagtcg
     7621 ggttattacg ttgcgccaca ggcaataaaa atgccgaaag cgccagtcat tccagtcact
     7681 cgagatgaag cagtcgatat caacacctat atttttgata tgttgcaggc cagtcgcgat
     7741 ccgtcggtag ttccgtttgc ctcggccttt cccgacccgc gacttttccc cctccaacaa
     7801 ctaaaccgct cgctggcgca ggtaagcaaa accgccacgg cgatgagcgt gattgaaaac
     7861 ttgccgccag gaaacgcaga actgcgtcag gctattgccc gtcgctatgc cttacagggc
     7921 atcaccattt ctcctgatga aattgtcatt actgccgggg cgttagaggc attaaacctc
     7981 agtttgcaag cggtaactga accgggcgat tgggtgatag tagagaatcc ttgtttctac
     8041 ggcgcgttgc aggcgctgga gcggctacgg ctgaaggcgt tatcggtggc gacggatgtt
     8101 aaagaaggga tcgatcttca ggcgctggaa ctggcgttgc aggagtatcc ggtgaaagcg
     8161 tgctggctga tgactaatag ccagaatcca ctcggattta ccttaacgcc gcaaaaaaaa
     8221 gcacaactgg tggcgttgct caatcagtac aacgtagcgc tgattgaaga tgacgtttac
     8281 agcgaacttt attttggacg ggaaaaaccg ctgcctgcga aagcgtggga tcgccacgat
     8341 ggcgttttgc attgctcttc gttttcgaaa tgtctggtgc ctggttttcg tattggttgg
     8401 gtcgccgccg gaaaacatgc acgtaaaatt caacgattgc agttgatgag tacgctttcc
     8461 accagctcac cgatgcagct tgcgctggta gattaccttt ccacgcgccg atacgacgcc
     8521 catcttcgtc gcctgcgtcg ccagcttgcg gaacgtaaac aacgtgcctg gcaggcactg
     8581 ctgcgttatc tgcctgcgga agtgaaaatt catcataatg acagcggcta ttttctctgg
     8641 ctggagctcc ccgaaccgtt agatgccggc gaattaagcc tggcggcact gacgcatcat
     8701 atcagtattg cgccgggtaa aatgttttct accggtgaaa actggtcacg tttttttcgt
     8761 tttaataccg cgtggcagtg gggagaacgt gaagaacagg cggtaaaaca attaggcaaa
     8821 cttattcaag aacggctgta atagcgttta atttaattcc tcttagattg ggtaatatga
     8881 atttcgaata gcagtcatat ttcctaactc cttgactata ctccagaaga taaccttaca
     8941 gacggcataa tgcgcggtag ctcacaacct gattaaattt tctcaggggc gaaggtgtgc
     9001 ctgcaagccg ccgtctatgg ttaaacaagg agatattttt acggcacggc ggctgaacaa
     9061 ttaattacga caggagtaag accttatgag caagacattt gcccgcagca gcctgtgtgc
     9121 gctcagcatg acaataatga ccgctcacgc cgccgaaccg cctaccaatt tagataaacc
     9181 ggaagggcga ctggatatta tcgcctggcc gggatatatc gaacgcggac aaactgataa
     9241 acaatacgac tgggtaacgc aattcgaaaa agagacaggc tgcgcggtga atgttaaaac
     9301 cgccgcgact tccgatgaaa tggtcagtct gatgaccaaa gggggttacg atctggttac
     9361 ggcatccggc gatgcctcgc tgcgtttgat tatgggtaaa cgcgtgcagc cgattaatac
     9421 cgccttgatt accaactgga aaacgctcga tccgcgcgtg gttaaaggcg actggtttaa
     9481 cgttggcggc aaagtttacg gtacacctta ccaatggggg ccgaacctgc tgatgtacaa
     9541 cactaaaacc ttcccgacgc cgccggatag ctggcaagtg gtttttgttg agcaaaatct
     9601 gcaggacggc aagagcaata aaggccgcgt tcaggcttat gatggcccta tctacattgc
     9661 ggacgctgcg ttgttcgtta aagccactca gccgcagttg ggcatcagcg atccgtatca
     9721 actcaccgaa gaacagtacc aggcggtgct gaaagtgctg cgcgctcaac atagtttgat
     9781 ccatcgctac tggcatgaca ctaccgtgca aatgagcgat ttcaaaaacg agggtgtggt
     9841 tgcttccagt gcatggccct atcaggccaa cgccttgaaa gccgaaggcc agcctgttgc
     9901 taccgttttc ccgaaggagg gtgttaccgg ttgggctgat accaccatgc tgcatagcga
     9961 agcgaaacat ccggtttgcg cctacaaatg gatgaactgg tcattaacgc caaaagtgca
    10021 gggcgatgtg gcggcctggt ttggctcgtt accggtagtg ccggaagggt gtaaagccag
    10081 tccgttatta ggcgaaaaag gttgtgaaac caacggtttt aactatttcg acaaaatcgc
    10141 cttctggaaa acgcctatag cagaaggggg caagtttgtt ccctacagtc gctggacgca
    10201 ggattacatt gccattatgg gcggtcgcta acttcgctgg ggtgctttat gacgtacgca
    10261 gtggagtttg acaacgtctc gcggttgtac ggtgacgtgc gcgcagtaga tggcgtcagt
    10321 attgcgataa aagatggtga gttcttctct atgctggggc cgtccggctc cggcaaaacc
    10381 acctgcctgc gcctgattgc tggcttcgaa cagctttccg gcggggctat ctctatcttt
    10441 ggtaaacccg ccagcaatct gccaccgtgg gagcgggacg tgaatactgt ctttcaggac
    10501 tacgcgctat ttccgcatat gtcgattctt gacaatgtcg cctatgggct gatggtcaaa
    10561 ggcgtgaata aaaagcagcg gcacgcaatg gcgcaagagg cgctggagaa agtggcgttg
    10621 gggtttgtac agcaacgtaa accgtcacaa ctttctggtg gtcagcgcca gcgggttgct
    10681 atcgccagag cattggtgaa tgaaccgcgc gtattgctgt tggatgaacc gctcggcgca
    10741 ctggatctca aattgcgtga gcagatgcag ctggaactga aaaaactgca acagtctctc
    10801 ggtatcactt ttatcttcgt tacccacgat cagggcgaag cattatcgat gtccgatcgt
    10861 gtggcggttt tcaataatgg acgcattgag caggtcgatt ccccgcgcga tctctatatg
    10921 cgcccgcgca cgccgtttgt tgccgggttc gttggtacat cgaatgtttt tgatggactg
    10981 atggcagaga aactttgtgg catgacggga agcttcgccc tgcggccgga acatatccgc
    11041 ctcaacaccc ctggtgaact gcaggccaat ggcacgatcc aggcggtgca atatcagggc
    11101 gcggcaactc gttttgaact gaaattgaac ggcggtgaaa aactgctggt gagtcaggcc
    11161 aatatgacag gcgaagaact gcctgccacg ctcacgcccg gacaacaggt gatggtttcc
    11221 tggtcgcgtg atgtgatggt gccgctggtt gaggagaggt gaatggcgat gaacgtattg
    11281 caatcacctt cacgtccagg tctgggtaag gtgtccggtt tcttctggcg gaatccgggg
    11341 ctggggctgt ttttgctgct gcttggcccg ctaatgtggt ttggcattgt ctatttcggc
    11401 tcgctgctaa cgctgttatg gcagggcttt tatacctttg acgatttcac catgtcggta
    11461 acgccggaac tgacgctggc gaacatccgt gcgctgttta atccggcgaa ttacgacatc
    11521 attctccgca cgctgactat ggctgtggca gtcactatcg ccagcgccat tctggctttt
    11581 ccaatggcgt ggtatatggc gcgctatacc agcgggaaaa tgaaagcgtt tttttatatt
    11641 gcggtaatgt tgccgatgtg ggcgagctac attgttaaag cctatgcctg gacgttattg
    11701 ctggcaaaag atggcgtggc tcagtggttt ttacaacatc ttgggctgga accactgctg
    11761 acagcgttcc ttacattacc tgcggtgggc ggcaatacgc tgtcaacttc cgggctgggg
    11821 cgctttctgg tgtttctcta tatctggctg ccgttcatga tcctgcccgt gcaggcggcg
    11881 cttgagcgtt tgccgccgtc attgttgcag gcgtcagctg atctcggcgc acgtccacga
    11941 caaacctttc gctatgtggt gctgccgctg gcgatcccgg gtattgccgc tggctctatc
    12001 tttaccttct cactcacact gggcgatttt atcgtcccgc agctggttgg cccgccagga
    12061 tattttatcg gcaatatggt ttattcccag cagggggcga ttggcaatat gccgatggcg
    12121 gcggcattca ccctggtgcc gattgttctt atcgcactgt acctggcgtt cgtgaaacgt
    12181 ctgggagcgt tcgatgcact ctgaacgcgc accgtttctc ctcaaactgg cggcctgggg
    12241 cggcgttgtt ttcctacatt ttcccatcct gataatcgcc gcctatgcgt ttaacactga
    12301 agatgcggca tttagttttc caccgcaagg cctgacgctg cgctggtttg gcgtggcagc
    12361 acagcgtagt gatattcttg attccgtgac actgtcactt aaagtggcag cgctggcgac
    12421 attaatcgcg ctggtgttag ggacgctggc agctgccgcg ctgtggcgac gagacttttt
    12481 cggcaaaaac gccatttcgc tgttactgct gctgcccatt gcgctgccgg gcattgtcac
    12541 tggtctggcg ttattaaccg cctttaaaac catcaatctg gagccgggat ttttcaccat
    12601 cgtggtcggt catgcgactt tttgcgtagt ggtggtgttt aacaatgtca tcgcccgttt
    12661 tcgccgcacc tcctggagtc tggttgaggc gtcaatggat cttggggcca atggctggca
    12721 aaccttccgc tacgtagtgt tgccgaatct cagttcggcg ttactggcag gaggaatgct
    12781 ggcgtttgcc ttgtcgttcg atgaaatcat cgttacgacc tttacggcag gtcatgaacg
    12841 aacgttaccg ttgtggttgc tcaatcagct tgggcgaccg cgtgatgtac cggtaaccaa
    12901 cgtggtggca ctgctggtta tgttggtaac aaccttgccg atcctggggg cctggtggct
    12961 aacccgcgaa ggcgacaatg gtcaataacc actgatacag gaaaatgcta tgcaacataa
    13021 gttactgatt aacggagaac tggttagcgg cgaaggggaa aaacagcctg tctataatcc
    13081 ggcaacgggg gacgttttac tggagattgc cgaggcatcc gcagagcagg tcgatgctgc
    13141 tgtgcgcgcg gcagatgcag catttgccga atgggggcaa accacgccga aagtgcgtgc
    13201 ggaatgtctg ctgaaactgg ctgatgttat cgaagaaaat ggtcaggttt ttgccgaact
    13261 ggagtcccgt aattgtggca aaccgctgca tagtgcgttc aatgatgaaa tcccggcgat
    13321 tgtcgatgtt tttcgctttt tcgcgggtgc ggcgcgctgt ctgaatggtc tggcggcagg
    13381 tgaatatctt gaaggtcata cttcgatgat ccgtcgcgat ccgttggggg tcgtggcttc
    13441 tatcgcaccg tggaattatc cgctgatgat ggcagcgtgg aaactggctc cggcgctggc
    13501 ggcagggaac tgcgtagtgc ttaaaccatc agaaattacc ccgctgaccg cgttgaagtt
    13561 ggcggagctg gcgaaagata tcttcccggc aggcgtgatt aacgtactgt ttggcagagg
    13621 caaaacggtg ggcgatccgc tgaccggaca tcctaaagtg cggatggtgt cgctgacggg
    13681 ctctatcgcc accggcgagc acatcatcag ccataccgcg ccctccatta agcgtactca
    13741 tatggaactt ggtggcaaag cgccagtgat tgtttttgat gatgcggata ttgaagcagt
    13801 ggtcgaaggt gtacgtacat ttggctatta caatgccgga caggattgta ctgcggcttg
    13861 tcggatctac gcgcaaaaag gcatttacga tacgctggtg gaaaaactgg gtgctgcggt
    13921 ggcaacgtta aaatctggtg cgccggatga cgagtctacg gagcttggac ctttaagctc
    13981 gctggcgcat ctcgaacgcg tcagcaaggc agtagaagag gcgaaagcga cagggcatat
    14041 caaagtgatc accggtggtg aaaagcgcaa gggtaatggc tattactatg cgccgacgct
    14101 gctggctggc gcattacagg acgatgccat cgtgcaaaaa gaggtatttg gtccagtagt
    14161 gagtgttacg cccttcgaca acgaagaaca ggtggtgaac tgggcgaatg acagccagta
    14221 cggacttgca tcttcggtat ggacgaaaga tgtgggcagg gcgcatcgcg tcagcgcacg
    14281 gctgcaatat ggttgtacct gggtcaatac ccattttatg ctggtgagtg aaatgccgca
    14341 cggtgggcag aaactttctg gttacggcaa ggatatgtca ctttatgggc tggaggatta
    14401 caccgtcgtc cgccacgtca tggttaaaca ttaaggataa tattgcagat cgtaagagta
    14461 tgagatgatc ttgcgtactg tcagagcact gatttctctg acagtacgtg ctgcgtgcaa
    14521 aatcactaca tcaaataaac cagccaaatc tcatagagat aagagtaaac aaaccaacag
    14581 cagcaaagac atttatcagt actacagttc tactggaaac attcattttt gccacctttt
    14641 atcattgccc ctatgtgtat agtacgggtt aagaaaatcc gcaataatac tcattctgaa
    14701 gatggataag ggcaagttgc tgtttgatga ttttttctca aaccggtgaa aaaacttgat
    14761 gcacgtcaaa aaatgacgca tatttgcgcg cgttttattc atctggctgg acgcccgtac
    14821 atgtctctct atcaacatat gcttgttttt tatgcggtta tggcagcaat cgcatttctt
    14881 atcacctggt ttctttctca cgataagaaa cgcatccgtt tcttaagcgc ctttctggtg
    14941 ggggcaacat ggccgatgag tttcccggtg gcgctgttgt tttcactgtt ttaaatctta
    15001 ctcactggct tgacggcacc acgcagactt atatcatttg gatgaatcga taaatttcac
    15061 aagtggctaa ggagaaagta tgtcgcatct ggatgaagtc atcgcgcgcg tggatgccgc
    15121 gattgaagag agcgtcattg cccatatgaa cgaattatta atcgccctga gcgatgacgc
    15181 ggagttaagt cgggaagatc gctacaccca gcagcagcgt ttgcgcaccg ctattgctca
    15241 tcacggtcgc aagcataaag aagatatgga agcgcgccat gaacagttaa ccaaaggcgg
    15301 caccatcctg tgattaaaat gagcgtctgg ctaccaacca ggcgccaacc acaagtaaaa
    15361 ttgcgccaca aattgggccg aacaacgccc gtagtggtac gccatgactg cggaagatat
    15421 ccagaaccag accgccaatc agctggctgg cgaccagtac tgcaatagtt gtcgccgctc
    15481 ccacattctg atatccgctg atactggcga agacaaaaaa cgatcccagc aagccaggta
    15541 taagtgtcca ccagcgcacg ctggacacca gttcgccaaa ccccgccata ccttgtttaa
    15601 accataaaat agaaacaaag agcacaatcc ccaccagtga gttcagcaac atggcgatga
    15661 gaatggtaga ggaggtctgg gtgatgcgca ccatcagcgt gttctggact accagaccaa
    15721 ttccggcggc aatcaaaaag gcaagggtga gcgactgatt catccaatcg cgtccggttc
    15781 agtgcgttcg tcgagttgca actgcataaa tgtcagatcc agccagcgcc caaatttagt
    15841 gcccacctgc ggcatttgtg cggtgacgac aaatcccagc gactggtgca gatgcagcga
    15901 ggcctgattt tgcgattcga tcccggcgac catgacatgc ttcccgcaat cccgcgcttc
    15961 atcaatcaat cggcttaaca atttacgccc cagacctttg ccctgatgat cgggatggac
    16021 ataaaccgaa tgttccacgg tatggcggaa accatcgaaa ctacgccagt cgccaaatga
    16081 ggcatatccc gtcactacgc catcttcttc acttaccagc accggataac ctgctatggt
    16141 ccgcgcttca aaccaggcaa tgcggttatc tgcatcaacc gtttggtcat tccagatagc
    16201 cgccgtatac aacacggcgt ggttataaat ttccgcaatg gcagcacaat cggctttgcg
    16261 ggcaaaacgg atggacatgt ctggcctcga taaaataatg atgatgatgt tatcaatact
    16321 atcgaaaaca gcccgaagca acggattcct cgatgaaatt attgcgtgtt gcagagggga
    16381 tgtgacggct gcaaacaaaa ccaatcccgt gagcctgcta ctattgatgg attcatgcca
    16441 aaagcacagg agagcattat ggggcaacaa aagcagcgta atcgacgttg ggttctggcc
    16501 tcgcgtccac atggcgcacc ggttccggag aatttccgtc ttgaagaaga tgatgtcgcc
    16561 acaccgggtg aaggacaggt gttactgcgc acagtttatt tgtccctgga cccgtatatg
    16621 cgtggacgta tgagcgatga gccatcttat tcaccgcctg ttgatattgg cggcgtgatg
    16681 gtcggcggca cggtgagccg tgttgtggag tcgaatcatc ctgattatca gcctggcgac
    16741 tgggtgctgg gctacagtgg atggcaggac tatgacatat ccagtggtga tgatctggtg
    16801 aaacttggcg atcatccgca aaatccatcg tggtcgctgg gtgtgctggg gatgccaggc
    16861 tttaccgctt atatggggct gctggatatc ggtcagccta aagagggcga aacgttggtg
    16921 gtagctgcgg cgacaggtcc tgtgggagca acggtggggc aaatcggcaa acttaaaggt
    16981 tgcagggtgg tgggggttgc cggtggcgcg gaaaaatgcc gccatgctat cgaggtgctg
    17041 ggcttcgatg tttgtcttga tcaccacgcg gatgattttg ccgaacaact ggtgaaagcg
    17101 tgcccaaaag gtattgatat ctattatgaa aacgtgggcg gtaaggtatt tgatgcagtg
    17161 ctaccattac ttaatacatc tgcgcgcatt cccgtttgtg gcttagtgag cagctataac
    17221 gctacagagc taccacccgg tccggatcgt ttacccctgt tgatggcaac ggtgctgaaa
    17281 aaacgtattc gcttgcaagg gtttattatc gctcaggatt atggtcaccg catccatgag
    17341 tttcagaagg agatggggca atgggtgaaa gaggataaaa tccactacca cgaagatatt
    17401 actgatggtt tagagaatgc gccacagacg tttatcggcc tgctgaaggg taaaaacttc
    17461 ggcaaagtgg tgatccgcgt cgcgggtgat gattaaagtt tcaacggcgg cgtaagccgc
    17521 cataaaaatc caggcaaaaa gcttattttc agctttaatt aactaaccac gtcattgttc
    17581 tgctgctaat aacaacaaag gtgggtggtt tatgattatt cactttaata caccaggtga
    17641 actccttctg ccgtgcaggc agggttggac agaaaacacg cgaaggaatt aaaatgcctg
    17701 gaatgggaaa aatgaaacat gtcagtttga ctctgcaggt tgagaacgac ctgaaacatc
    17761 agcttagtat tggcgcatta aaacctggcg cacgcctgat tactaaaaat ctggcggagc
    17821 aattaggtat gagtattaca cctgtgcgtg aagcattatt acgtctggtt tcggtgaatg
    17881 cgctttctgt cgcacctgca caagcattta cagttccgga agtggggaaa cgtcaattag
    17941 acgaaatcaa tcggatccgc tacgaactgg aattaatggc agttgctctg actgttgaaa
    18001 acctcacccc gcaagacctg gcggaactcc aggaattgct cgagaaatta cagcaggcgc
    18061 aagaaaaggg cgacatggaa caaatcatta atgtaaacag gctatttcgc ttagcgattt
    18121 atcatcgctc aaatatgccc atcctgtgtg agatgattga gcaactgtgg gtcaggatgg
    18181 ggcctggttt acattatctc tatgaagcga ttaatccagc ggaattacgg gagcatatag
    18241 aaaactatca tctgttactc gctgcgttaa aagcaaaaga caaagaggga tgcagacatt
    18301 gtcttgctga aattatgcaa caaaatattg ctattttata ccagcaatac aatcgttaaa
    18361 gtaaatgccc ggcaaatacc gggcatcaag gtaattactc aaatctccac gcaatattca
    18421 tgccgacacc ataatttcgt ccgggcgaag gttcgtaata tcgcccgttt gactcattga
    18481 caatgacaga accaacgtat tctttatcga ataaattatc gacacgacca aataaatcca
    18541 cagttaaatt gtggtaattg tatttatacc cggtgaataa gccgacgaga gtataagacg
    18601 gcgcttttgc cgtattttca tcatctgcca taatatcgcc catataacgc gcttccgtgc
    18661 ctgcatacca accatcttcc ggtacataac ctatcgacgc aaagcccata ttacgggcga
    18721 tccctggcat ccgattaccg ttacaatcct gttcattgca aacattgctg cgataggtcg
    18781 catcaagcca ggtccaggac gcgtttacgc gaaaatctcc tgcgaaacgt tgatcccatg
    18841 ccagttcagc gccttgacga cgggtctttc cggcattttt gtaagtcgta cgcccaccgc
    18901 tactgctatc gacaacaatt tcatcatcag tgtcggtctg aaacaatgcg agactaagca
    18961 gcccatcacc aatacgcgtt ttactgccga tctcaattgt atcgttggtg gatggtttta
    19021 aaccaaagtt cataccgctt tgcccatcag cacgataaga cagctcatta atcgtcggcg
    19081 tttcaaaacc tcgcccggct gccagataga tattccaggc atcggtcatt gcatatttta
    19141 acgaaccggc aggtagccat ttatgataac tggcatcacc gctgtcatcg ccgttacccg
    19201 gagtaacgta atggtcgttg gaatcaaacc acacggagct gtagcgcacg ccagcatcca
    19261 gcgacagttt ttcgctcagc tgccactgcg tctgtaaata gggatcgatg ttccacatca
    19321 gattgcgttc gtcgcgacgc aactcacctt tttgcccgta ctccggcatg ccgctattca
    19381 ggcggaagtt attgtagccc ttgcggtttt cactcatgtt ttcgtagttc aggccggtag
    19441 tgaacgtgac cggaacgccc agttcaccac ggtgtgtcca gcggctgtct atcccctggt
    19501 aatggcgttg cagggtaatc acgccgcccg catgtgacgg gttaagttgt ggtgccatgg
    19561 gtattgactg gtactgggtc gtttctcgct ctccggcata catcatcaca ctcatatcat
    19621 cttgcgcgct caggctacgt tcatagcgca acccagcctg agtttgcttg atggtttttc
    19681 gcgtgtcgta ctgttcagcg cgtggcgcgt gttgcggatt cgccttccat tctgcttcgg
    19741 ttagcccacc tgggtcatct gctttgatat ccacgctatt gaaaatcagg cttactttgc
    19801 tggcgtcatc aatgcgtacg cccagtttgg cattggctaa atttttctgt gcgccgctat
    19861 ggtcacgata gccgtgggtc gtaaaacgcg tggttgagac ggtgtaatcg acatcgccgg
    19921 gctgtgtgcc gtcgcccgtt gcacccgttg ctttgagccc atagcgccag ctgccaaaac
    19981 tgccgtagta actgctggct tcaatggttg gtggctgttg cccggtctgg gtggtgacat
    20041 tcattacacc gccagacgcg ttgccataca gggcagagaa ggggccacgc agcacttcca
    20101 cattttgcac actgcttaaa tcgatgttgg atgtttgccc ttgcccgtcg ggcatggtag
    20161 cgggaatacc gtccacatac aggcgaatac cgcgaattcc gtaagtggag cgggagccaa
    20221 atccgcgaat cgacagctgt aaatcttgcg catagttctg ccgattttgt acctgcaaac
    20281 caggcacgct ggtcagtgat tcggacaagt taatgcgcgg tgttgccagg cgcatctcct
    20341 cgccatccac cacgcttact gctgctgggg tatccagttc tgaaaccacc tgcggtgcgg
    20401 cactgacaat catggtctgt tcatcagcgg caaaaacaac gggggaaagg gcaagtagtg
    20461 cgggcaaaac ggtctgtcgg acggaaaaaa tcttcatgaa aaaagccagg ttaagaatgg
    20521 gaaaacgccg ttatggtaat gaaattgtaa atttatggaa aacgaaacgg cacgatacgt
    20581 taagtaattg agaaaattgt agtcgtaacg gcaagaaatg ctccacattt gagaaaataa
    20641 tgattaccat tcccatttat aacaagagcg taacgatgat tacgcttagc gaagcattgt
    20701 gaagcagcaa aaatatcggt tcatcaaagg gagtcgtcat gcatttacgt catctgtttt
    20761 catcgcgcct gcgtggttca ttactgttag gttcattgct tgttgcttca tcattcagta
    20821 cgcaggccgc agaagaaatg ctgcgtaaag cggtaggtaa aggtgcctac gaaatggctt
    20881 atagccagca agaaaacgca ctgtggctcg ccacttcgca aagccgcaaa ctggataaag
    20941 gtggcgtggt ttatcgtctt gatccggtca ctctggaagt gacgcaggcg atccataacg
    21001 atctcaagcc gtttggtgcc accatcaata acacgactca gacgttgtgg tttggtaaca
    21061 ccgtaaacag cgcggtcacg gcgatagatg ccaaaacggg cgaagtgaaa ggccgtctgg
    21121 tgctggatga tcgtaagcgc acggaagagg tgcgcccgct gcaaccgcgt gagctggtag
    21181 ctgatgatgc cacgaacacc gtttacatca gtggtattgg taaagagagc gtgatttggg
    21241 tcgttgatgg cgagaatatc aaactgaaaa ccgccatcca gaacaccggt aaaatgagta
    21301 ccggtctggc gctggatagc aaaggcaaac gtctttacac cactaacgct gacggcgaat
    21361 tgattaccat cgacaccgcc gacaataaaa tcctcagccg taaaaagctg ctggatgacg
    21421 gcaaagagca cttctttatc aacattagcc ttgataccgc caggcagcgt gcatttatca
    21481 ccgattctaa agccgcagaa gtgttagtgg tcgatacccg taatggcaat attctggcga
    21541 aggttgcggc accggaatca ctggctgtgc tgtttaaccc agcgcgtaat gaagcctacg
    21601 taacgcatcg tcaggcaggt aaagtcagtg tgattgacgc gaaaagctat aaagtggtga
    21661 aaacgttcga tacgccgact catccgaaca gcctggcgct gtctgccgat ggcaaaacgc
    21721 tgtatgtcag tgtaaaacaa aaatccacta aacagcagga agctacccag ccggacgatg
    21781 tgattcgtat tgcgctgtaa tagatttggc gagggtgaaa acggagagac ttgcctgact
    21841 tttttcacgc cgtatccggc actttcgtca gtaatctgaa tttgcccggc acgtagccgg
    21901 gcaattttgc aattaactcg ccgtttcagg cttaaacaca atttcctgtt tttcttcatc
    21961 ttcctcgacg actggcgcag tgctgtgaat ttcggcaacg cgtttgcgca caccaaacca
    22021 acccacaacc agcagaaggc caataatcgg cagcgaagca atggtataag tcccgttcgg
    22081 gtaatcgaac gccatcagca ccagtacgct cagcaaaaac aataacgtca gccaggaagt
    22141 gaagggggca ccggggagtt taaaactgac atctgccgct ttaccttctt taatagcctt
    22201 acgcagacgc atctggcaaa cgatgataaa cgcccatgaa gcgatgattc ccagcgacgc
    22261 gaagttcaac acaatctcaa atacgcgtga cggcaccaga tagttgagga atacgccgac
    22321 gacatacaca accagtgtcg ccagaatccc ggcatacggc acatgctgac ggctcatttt
    22381 cgccataaaa ctcggtgcgg aaccgcccat cgccattgag cgcagaatac gcccggtgca
    22441 gtacaggcct gagttcaggc tggagagtgc agcggtcagc accacaatat tcataatgct
    22501 gccgatatac ggcacgccaa gtttagagaa gaaagtcaca aaaggactct gcccggcctg
    22561 gtatgcattc cacggcagca atagcaccag cagaaccacc gaacccacat agaacagacc
    22621 gatacgccaa ataacactgt tgatcgcttt cggcaccatc gtctgtggat ctttacattc
    22681 accagccgca gtacctacca gttcaatcga tgcaaaggca aaaaccaccc cctggataag
    22741 caccagtgca ggcagcagcc cgtgcgggaa gaaaccgcca ttatcggtaa ttaaatggaa
    22801 ccctgtggta ttaccatcca gcggctgacc actaccgagg aacaccgtac ccaccaccag
    22861 gaagatcaca atagcgagca ctttaatgag tgcaaaccag aactccatct ctgcgaacca
    22921 tttcacaccg atcatgttca tggtgcctac gatggtcagc gcagcgagcg caaagaccca
    22981 ctgcggcacg tcgccaaacg ccccccagta atgcatatac agcgctacag cggtgatatc
    23041 gacaatcccc gtcatcgccc agttgatgaa atacatccag ccagcaacat aagcggcttt
    23101 ctcacccaaa aactcacggg cataagagac aaaactgcca ctggaagggc ggtgtaacac
    23161 cagctcaccc aatgcacgta gaataaaaaa tgaaaacagg ccgcagatca aatagaccag
    23221 tgccagtgcg ggccccgcca tttgcagtcg ggctcctgca cctaaaaaca agccggtgcc
    23281 aatcgcgccg ccaatggcga tcatctgtac ctggcgattg cccatcgctt tgtgataccc
    23341 ctcttcgtgg gcattaagcc agcggcgttt cgcggcgtgt tgatctgaag tgtcggtgtc
    23401 gtgtttactc attgctctcc ctgattgctt taatgaaaaa gtcatataag ttgccatgaa
    23461 caatgtttat tcatcctggc gatgatagtt atcaccatta ttctttatgg gtataaaact
    23521 atcggcgcag catcctaccc gttatcgata aacgatgcaa aacatcccct tacaatcctg
    23581 aaggggaata atacaactga cgaaaaaatg acaaatcctt ttgctggtta acctgtgtac
    23641 tgtcctacac ttaatcttta aaagattgtg aggggcatat gattaaggtg tatggcgtac
    23701 ccggatgggg ctctacaatc agtgagctga tgctaacact ggctgatatt ccttatcagt
    23761 tctttgatgt cagtggtttt gaccacgagg gaacttcacg cgagttattg aaaaccctga
    23821 acccgttgtg tcagattccg acactggcgc tggaaaatga cgagattatg acggagacag
    23881 cggcgattgc attgatggtt ctcgatcgtc gcccggacct tgcgccaccg gttgggcgcg
    23941 ccgaacgtca gctgtttcaa cggctattgg tctggctggt tgctaatgtc tatccaacat
    24001 tcactttcgc cgattaccct gagcgttggg ctcctgacgc acctgaacag ttaaagaaga
    24061 atgttattga atatcgaaaa tcgctttata tctggttgaa ttcgcagctc actgctgaac
    24121 cttatgcgtt tggtgagcaa ctaacgctgg tggactgtta tctttgcact atgcgcacat
    24181 gggggcctgg gcatgaatgg tttcaggata atgccacgaa tatcagtgcg attgctg
//
LOCUS       DAPGOF010000067        23962 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.067, whole genome shotgun sequence.
ACCESSION   DAPGOF010000067 DAPGOF010000000
VERSION     DAPGOF010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.067"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(57..1247)
                     /gene="bcr"
                     /locus_tag="RHV13_002309"
     CDS             complement(57..1247)
                     /gene="bcr"
                     /locus_tag="RHV13_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HDV1241837.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(1275..1970)
                     /gene="rsuA"
                     /locus_tag="RHV13_002310"
     CDS             complement(1275..1970)
                     /gene="rsuA"
                     /locus_tag="RHV13_002310"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HDV1241838.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            2119..3879
                     /locus_tag="RHV13_002311"
     CDS             2119..3879
                     /locus_tag="RHV13_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDV1241839.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRHHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAATRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            4004..4288
                     /gene="rplY"
                     /locus_tag="RHV13_002312"
     CDS             4004..4288
                     /gene="rplY"
                     /locus_tag="RHV13_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HDV1241840.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(4427..5434)
                     /gene="yejK"
                     /locus_tag="RHV13_002313"
     CDS             complement(4427..5434)
                     /gene="yejK"
                     /locus_tag="RHV13_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HDV1241841.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            5616..5843
                     /locus_tag="RHV13_002314"
     CDS             5616..5843
                     /locus_tag="RHV13_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HDV1241842.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            5863..7623
                     /gene="pbgA"
                     /locus_tag="RHV13_002315"
     CDS             5863..7623
                     /gene="pbgA"
                     /locus_tag="RHV13_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HDV1241843.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFAAYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            7698..7774
                     /locus_tag="RHV13_002316"
     tRNA            7698..7774
                     /locus_tag="RHV13_002316"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7732..7734,aa:Pro,seq:ggg)
     gene            complement(7876..10467)
                     /gene="yejO"
                     /locus_tag="RHV13_002317"
     CDS             complement(7876..10467)
                     /gene="yejO"
                     /locus_tag="RHV13_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HDV1241844.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVVLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGISSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGAANDPTIKGGRLIVEKDGIAVFAAIEKGGLLEVKDGG
                     LAFAVDQKAGGAIKASTRVMEVFGTNRLGQFEIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTRVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMDEPVTRS
                     SVTASAENFTTLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTAMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVLKVDRFANTIHGKMSNGATAFGDYSSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTRGVYQAGIRSSFTPTLSGHLSVSYGNGAGVELPWNTQ
                     AGVVWTF"
     gene            10787..11434
                     /gene="narP"
                     /locus_tag="RHV13_002318"
     CDS             10787..11434
                     /gene="narP"
                     /locus_tag="RHV13_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HDV1241845.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLHEREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(11645..12697)
                     /gene="ccmH"
                     /locus_tag="RHV13_002319"
     CDS             complement(11645..12697)
                     /gene="ccmH"
                     /locus_tag="RHV13_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HDV1241846.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVYYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRSDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(12694..13251)
                     /gene="dsbE"
                     /locus_tag="RHV13_002320"
     CDS             complement(12694..13251)
                     /gene="dsbE"
                     /locus_tag="RHV13_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HDV1241847.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(13248..15191)
                     /gene="ccmF"
                     /locus_tag="RHV13_002321"
     CDS             complement(13248..15191)
                     /gene="ccmF"
                     /locus_tag="RHV13_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HDV1241848.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(15188..15667)
                     /gene="ccmE"
                     /locus_tag="RHV13_002322"
     CDS             complement(15188..15667)
                     /gene="ccmE"
                     /locus_tag="RHV13_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HDV1241849.1"
                     /translation="MNIRRKNRLWIACAVLAGLVLTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(15664..15873)
                     /gene="ccmD"
                     /locus_tag="RHV13_002323"
     CDS             complement(15664..15873)
                     /gene="ccmD"
                     /locus_tag="RHV13_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HDV1241850.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(15870..16607)
                     /gene="ccmC"
                     /locus_tag="RHV13_002324"
     CDS             complement(15870..16607)
                     /gene="ccmC"
                     /locus_tag="RHV13_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HDV1241851.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(16649..17311)
                     /gene="ccmB"
                     /locus_tag="RHV13_002325"
     CDS             complement(16649..17311)
                     /gene="ccmB"
                     /locus_tag="RHV13_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HDV1241852.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(17308..17925)
                     /gene="ccmA"
                     /locus_tag="RHV13_002326"
     CDS             complement(17308..17925)
                     /gene="ccmA"
                     /locus_tag="RHV13_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HDV1241853.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLARATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(17944..18546)
                     /gene="napC"
                     /locus_tag="RHV13_002327"
     CDS             complement(17944..18546)
                     /gene="napC"
                     /locus_tag="RHV13_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HDV1241854.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(18556..19005)
                     /gene="napB"
                     /locus_tag="RHV13_002328"
     CDS             complement(18556..19005)
                     /gene="napB"
                     /locus_tag="RHV13_002328"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HDV1241855.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(19002..19865)
                     /gene="napH"
                     /locus_tag="RHV13_002329"
     CDS             complement(19002..19865)
                     /gene="napH"
                     /locus_tag="RHV13_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HDV1241856.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(19852..20547)
                     /gene="napG"
                     /locus_tag="RHV13_002330"
     CDS             complement(19852..20547)
                     /gene="napG"
                     /locus_tag="RHV13_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HDV1241857.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(20554..23040)
                     /gene="napA"
                     /locus_tag="RHV13_002331"
     CDS             complement(20554..23040)
                     /gene="napA"
                     /locus_tag="RHV13_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HDV1241858.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQDVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(23037..23300)
                     /gene="napD"
                     /locus_tag="RHV13_002332"
     CDS             complement(23037..23300)
                     /gene="napD"
                     /locus_tag="RHV13_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HDV1241859.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(23290..23784)
                     /gene="napF"
                     /locus_tag="RHV13_002333"
     CDS             complement(23290..23784)
                     /gene="napF"
                     /locus_tag="RHV13_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HDV1241860.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
BASE COUNT         5860 a         6810 c         5920 g         5372 t
ORIGIN      
        1 tgtggttgac atgcatcaac aaaggaagcc ttttagcttc ctcgttgtgc aatagatcac
       61 cgttttttcg gccgactggc gtacagacag aagagaatgg agctggttgc gcagaatgca
      121 attgaccaaa tcatcggcca tgcagagtta aaggtcgcaa gagaaagcaa tgcgccaaca
      181 attgccccta tgccaaaacg gaaggttcct gccagcgaag atgccgttcc cgccatatgg
      241 ggaaactcat caagaatgac cgccatcgca ttggatgaca ccatcgacac gcagcccaca
      301 aacgccgcaa cgccaaccac cagcgaccag aatcccagcc ccagcagcgc actgatgacc
      361 atccacgctg ccataataaa ttgtatccac aaccccgagc ggaacatatt taacgcgcca
      421 atgcggcgaa cgaagcggct attaaagatg gtcatcacga acaggaagac aatgtttagt
      481 gcaaagtaat aaccaaagtt ttccggcgcg acgtggttaa tttcaatgta gacaaacggt
      541 ccggcgctta agaacgagaa catcccggca aagctgaaac cactggccag catataactc
      601 aggacacgct tatgacggaa cagcgccgca aagttaccaa tagtggtacg aatgtgaaaa
      661 ggctggcgac gctccggtgg taaggtttct ttgatcagaa agaagatcat tgccgaagcc
      721 agaatcgccg ccacggcgag gatccagaag atgtaatgcc agctcagcca caccagcacc
      781 cagccgccaa ctatcggtgc catcagcggt gcaatggttg tcaccagcat gacaaacgac
      841 atcatccgcg agaactcttc tttcgggtag atatcacgca tcaaagcgtt gataaccacg
      901 ctggccgcag ccgccgccag cccgtggaag aaacgcatca caatcagctg atcgatggtt
      961 tgtgccaacg cacacgccac cgcggcggcg gcaaacacca gcgtaccgcc aagaaccacc
     1021 ggcttacgcc cgaagctgtc tgccatcggc ccgtagatta actgccccaa cgcaaagccc
     1081 agaatataag tactgagggt catctgcgta ctgcccgccg gtacgccaaa ctgcgctgaa
     1141 attaccggta gcgcgggcag atacatatca atcgacagcg gcatcaacat ggccagcagg
     1201 ccaaggataa aaacaatagc aaacgacgaa tgctgtcggg tggtcacaac gggctcctga
     1261 aagtcattga aaaattagac gacgctggca atttcttctt cagttaacgg acgatattca
     1321 ccgggggcta aatcagcatc cagcgtaatg ccgccaatac gttcacgatg cagctcaacc
     1381 acatggttac ccacagcggc gaacatgcgt ttcacctgat gataacgccc ttcgctgatg
     1441 gtcagacgaa cctgcgttgg ggtaatcact tccagcaccg ccggcttagt gagatctttt
     1501 tcgttatgca gctgcacgcc tttagcaaat tgctctgccg tatcgtcagc tacgggcgat
     1561 tccagcgtca ccagataggt cttctcgcaa tgatggcgcg gagaagtaat gcggtgcgac
     1621 cactgaccat catctgtcat tagtaccaga ccggtggtat cgatatccag ccgccctgcg
     1681 gcgtgcagtt tccacgccac tggttcatcc agaaaataga gcaccgttgg gtgatcaggg
     1741 tcgtccgtgg agcaaacata gccctgaggc ttattgagca tgaagtaacg tggaccgtgt
     1801 tgctgcgcca gcgggttgcc atcataagcg acatcatgtt caggaagcag tttgaacgct
     1861 gcattacgga cgatttcgcc atcgacggtg acacgattgc cgcggatttc acgcccggca
     1921 atagcacggc taacgccgag ttgctgtgcg ataaatttat caagtcgcat gtgtgtgatt
     1981 ttgcctgtaa aaacggaggt cgggcattga cccgaaaatc tgaactgttg tctgcccagt
     2041 atagcggtct aataacttcc ctcaagggaa aaagaaccgt ggcatactat tagcagaata
     2101 atctacctac gcgagatcat gatttttaca ctccgcccat atcagcaaga agccgtggat
     2161 gccacgctca accattttcg tcatcataaa acccctgccg ttatcgtgct gcccaccggt
     2221 gcaggtaaaa gcctggtgat agcggaactt gcgcggctgg ctcgtggtcg cgtgctggtg
     2281 ctggcacacg ttaaagaact ggtggcgcaa aaccatgcaa agtatcaggc gctggggctg
     2341 gaagccgata tttttgccgc cgggctaaag cgcaaagaga gccatggtaa agtggtattt
     2401 ggcagcgtgc agtcggtcgc ccgtaatctt gatgcctttc agggtgaatt ttcgctgttg
     2461 attgtcgatg aatgccaccg tattggtgac gatgaagaga gccagtatca gcaaatcctc
     2521 actcacctga caaaagtgaa tccccactta cgcctgctgg ggctgactgc cacgcctttt
     2581 cgactgggca aaggctggat ctaccagttc cattatcacg gcatggtacg cggcgatgag
     2641 aaagcccttt tccgtgactg catttatgaa ctgccgctgc gttatatgat taaacacggc
     2701 tatctgacgc cgccagaacg actggatatg ccagtagtgc aatacgattt cagccgcttg
     2761 caggcacaga gtaacgggct attcagcgaa gccgatctca accgcgagct aaaaaaacaa
     2821 caacgtatta ccccgcacat catcagccag attatggagt ttgctgcaac gcgcaaaggg
     2881 gtgatgattt tcgccgccac cgttgaacac gcaaaagaga ttgtgggatt actacccgcc
     2941 gaagatgctg cactgattac tggcgacacc cccggcgctg agcgcgatgt gttaattgaa
     3001 gattttaaag cccagcgttt tcgctatctg gtcaacgtcg cagtactgac caccggattt
     3061 gacgccccgc acgtcgatct tatcgccatt ctgcgcccta ccgaatcagt gagtctttac
     3121 caacaaattg tcgggcgcgg tctgcgtctc gctccgggca agactgattg tttaattctt
     3181 gattatgcgg gtaatcctca cgatctctac gcgccggaag ttggtacacc gaaaggcaaa
     3241 agtgacaacg tcccggtaca agttttctgc cctgcctgcg gttttgccaa caccttttgg
     3301 gggaaaacga ccgctgacgg gacattgatt gaacactttg gtcgtcgttg tcagggatgg
     3361 tttgaagatg acgacggtca tcgcgaacag tgtgatttcc gtttccgttt taaaaattgc
     3421 ccgcaatgta acgcggaaaa cgatattgcc gcccgccgct gccgcgaatg tgacacggta
     3481 ctggttgatc cggatgatat gttaaaagcg gcgctacgac tgaaagatgc gctggtatta
     3541 cgctgtagcg gcatgtcttt gcagcatggg cacgacgaga aaggcgaatg gttgaaaatc
     3601 acctattacg atgaagacgg tgcggatgtg agtgagcgtt tccgtctgca aacgcccgcc
     3661 cagcgaactg ccttcgagca gctttttatc cgcccgcata cgcgcacacc gggcatccct
     3721 ctgcgctgga tcaccgccgc cgatatcctc gcccagcaag ccttattgcg acacccggat
     3781 tttgtcgttg cccgcatgaa aggtcagtac tggcaagtgc gtgaaaaagt gttcgattac
     3841 gaaggtcgtt ttcgtcgggc gcacgaatta cgcggttaat ggtagttttg attgatgtat
     3901 aaggcgattg agtatagaat ctcgcccgct tttgcatacg caaagcagat cacttacctg
     3961 ttgctgggtc gcctgtagca ggattaattt aagagagaaa gaaatgttta ctatcaacgc
     4021 agaagtacgt aaagagcagg gtaagggtgc gagccgccgc ctgcgtgccg ctaacaagtt
     4081 cccggcaatc atctacggtg gcaaagaagc accgctggct atcgagctgg atcacgacaa
     4141 agtcatgaac atgcaagcta aagctgaatt ctacagcgaa gttctgacca tcgttgttga
     4201 cggtaaagaa atcaaagtta aagctcagga cgtacagcgt cacccgtaca aaccgaagct
     4261 gcagcacatc gacttcgttc gcgcttaatt gctgaataag ttgtaaaaaa ccccgctccg
     4321 gcggggtttt ttgtatctgc agattatgcc tgattacggt attgctattt tttgcaggcc
     4381 agataaggcg ttcacgccgc atctggcatt cagaacacaa aaccgattaa ttgccgccgg
     4441 acgtccggcg ctgcaattga tcgcgcaaat tcggcggcgt gcctttaata gtcagggtat
     4501 cggtcgccgg atcccagaaa atccgttcgc ccaacagcat cgcatcaaaa ttaatagtca
     4561 agccaccgcc gctaccggca aacttcgtca actgacgcag tgtgctacgg tctgccggga
     4621 agctctcttc cagttcgtag cctttttcag ccgcaaactc cgtgaagctc acttcgctga
     4681 cgcctgccag ctctttcgac agcgattcca gttcgatctc ttcgcctgcc tgcagctgct
     4741 cattacagta gctataaacc tgctggcgca cgttctgtcg ctctgcttta tccagttgtg
     4801 cttcggcagt gaaatcatca accgcttgca acagcccacg gttttgcgct ttcgcgttta
     4861 acccttcgct ggccccgagg aaatccataa agaaatccgc cactttgcgc cctactcgcc
     4921 ctttcaggaa agtgagatag cgggtggact ctggattggt ttcccattcg gttaaatcga
     4981 tacgcgcaac aatatccgca tgattgatat caagataatg ggttgggttg atgtcgagat
     5041 tttcgttaac gcgcatactg ctcaggttgc tcaggactgc caccagcaga tactccaccg
     5101 ccagataacg gtagtggcaa aacagcacga atccaccgtc agcaaaagga tatttcgcta
     5161 attcgtcacg caggcgaccg gttgccgcgc ggctaaatgc cagaaagtcc tcttcgccct
     5221 gacgctgtaa gcgcagcgtc tgcgccagtt cactctcttc gctaaacaag ccgtaagctt
     5281 tatttttggc gctatagacc cgatgcagtt ctgccaccat ctcaacgacg gtttctgtcg
     5341 gttccagcaa tgaatcgcgc aacaccagct caaggttctg ctcatcacgc ttgataagct
     5401 ggtgcagagc aatctggttg atatccagac tcatgataaa ctctccttta agaccgggcg
     5461 gtattcaacc accgccggtt aagcgacgca ataaaagatg gcggtcattt tacagaaggc
     5521 agcgtaacga gaatgattta acgcataaaa aagcagaaaa aaaaccgttg ctacggtaat
     5581 atgttgccct ttcatcaact aactgatttc gatttatgcc acaaatttcc cgctacagtg
     5641 atgaacaggt tgaacaattg ctcgcagagc tgctcaacgt actggaaaaa cataaggctc
     5701 cgaccgacct ttccctgatg gtgttaggca acatggtcac taaccttatc aacaccagca
     5761 ttgccccggc ccaacgccag gcaattgcca actcttttgc ccgcgcctta cagtcctcta
     5821 tcaacgaaga caaagcgcac taagggaaac agataacagg ttatggtaac tcatcgtcag
     5881 cgctaccgtg aaaaagtctc ccagatggtc agttgggggc actggtttgc actgttcaat
     5941 attctgcttt cgctcgtcat tggcagccgt tacctgttta tcgccgactg gccgaccacg
     6001 cttgctggtc gcatttattc ctacgtaagc atcatcggcc atttcagctt cctggtgttc
     6061 gccgcctact tgctgatcct cttcccgctg acctttatcg tcggctccca gaggctgatg
     6121 aggtttttgt ccgtcattct ggcaacggcg ggaatgacgc tattactgat cgatagcgaa
     6181 gtctttactc gtttccatct ccatcttaat cccatcgtct ggcaactggt tatcaaccca
     6241 gacgaaaatg agatggcgcg cgactggcag ctgatgttca tcagcgttcc ggttatttta
     6301 ttgcttgaac tggtgtttgc gacgtggagc tggcaaaagc tgcgcagcct gacgcgtcgt
     6361 cgacgcttcg cgcgcccgct agccgcattc ttatttatcg cctttatcgc ctcgcatgtg
     6421 gtgtatatct gggccgatgc caacttctat cgcccgatca ccatgcagcg cgctaacctg
     6481 ccgctttcgt acccgatgac ggcgcgacgt tttcttgaga agcatggcct gcttgatgcg
     6541 caggagtatc aacgccgtct tattgagcaa ggtaatccag acgccgtttc cgttcagtat
     6601 ccgttaagcg aactgcgcta tcgcgatatg ggcaccgggc agaatgtgct gttgattact
     6661 gtcgatggcc tgaactactc acgcttcgag aagcagatgc ctgcgctggc aggttttgct
     6721 gagcaaaata tttcgttcac gcgccatatg agctccggca acactacaga caacggcatc
     6781 tttggcctgt tctatggcat ctcgccgagc tatatggacg gcattctgtc gacccgtacg
     6841 cctgcggcat taattactgc gcttaatcag caaggctatc agctggggtt attctcatca
     6901 gatggcttta ccagcccgct gtatcgccag gcattgttgt cagatttctc gatgccgagc
     6961 gtacgcaccc aatccgacga gcagaccgcc acgcagtgga tcaactggct gggccgctac
     7021 gcacaagaag ataaccgctg gttctcgtgg gtctctttca atggtactaa cattgacgac
     7081 agcaatcagc aggcttttgc acggaaatat agccgggcgg caggcaatgt cgatgaccag
     7141 atcaaccgcg tgctcaatgc actgcgtgat tctggcaaac tggacaatac ggtagtgatt
     7201 atcactgccg gtcggggtat tccactgagc gaagaggaag aaacctttga ctggtcccac
     7261 ggtcatctgc aggtgccatt agtgattcac tggccaggca cgccggcgca gcgtattaat
     7321 gcgctgactg atcataccga tctgatgacg acgctgatgc aacgcctgct acatgtcagc
     7381 acacctgcca gcgaatattc gcaaggtcag gatttgttca accctcaacg ccgtcattac
     7441 tgggttaccg cagcggataa cgatacgctg gcaattacca ccccgaaaaa gacgctggtg
     7501 ctgaacaata acggtaaata ccgcacttac aacttacgtg gtgaaagagt gaaagatgaa
     7561 aaaccacagt taagtttgtt attgcaagtg ctgacagacg agaagcgttt tatcgctaac
     7621 tgattaatta taaatcagtt agcgaaatat cttacttgca atcggtgtgg aaaacggtag
     7681 tattaacagc cacgagtcgg cacgtagcgc agcctggtag cgcaccgtca tggggtgtcg
     7741 ggggtcggag gttcaaatcc tctcgtgccg accaaaaatc ccaaaaaaaa ccaaccgtaa
     7801 aggttggttt ttttatatct gcatttaatt cgataaacag accgcgacac atcacggcct
     7861 gtttattgtc tgttatcaga acgtccagac cacaccagcc tgggtattcc acggcaattc
     7921 tacccctgcg ccattgccat agctgactga caaatgaccg cttaacgtcg gggtaaacga
     7981 tgaccttatc ccagcctgat aaacgccacg ggttccagcc acatcattat taaatttgcc
     8041 atcgtcatta actttcacct ggttagaatc ggcgtattcc tgacgcacgg cggctttcag
     8101 ccagggttcc agcgtcgtac cgttttgcag gtccatgtga tagcttaccg ccgttcccgc
     8161 ttcagcgcgt aatatccggg tatttcccac atccgcgcgc atgccgtttg ataacgtgta
     8221 gtcctgacca tctgtggtaa agccggtaaa ggcgagatag ggtctaacac tccacaatcc
     8281 gtcaacccaa cggaacccgc tctcgacatg agcacctgcg ccgttactac tgtaatcgcc
     8341 aaacgctgtt gccccattac tcatcttgcc atggatggtg ttggcaaaac ggtcaacttt
     8401 cagcactcca tcaacatagg caccgttctg atgctcccaa ccggcataag cccccagggt
     8461 atagctatcg atattacctt ttccgccgcg atcaaaacca atatcagaat gagagtaacc
     8521 aaagaacaag ccgcgaattg tactgctttc ttcacgggag aaacggctat cgataccgag
     8581 cgtcaggccc gtcaatgttt gctcaaaacc agctcccgca tcagtggtca cgttgttgcg
     8641 ggtgttaatt gccgaactcc acatcgccgt atcgtaacta acgcctttta cgctaccaag
     8701 acgctcacgc acggtgtcca gttctgcatc aaataccagc ggttgtgcgg ccgccatatt
     8761 cagcacatca gtggttgaag gggtaatttg cgcgcgattc tctgccaaac tccagctatg
     8821 gttgccatta tccagcaagg tatattcata cgtaccgata tcaacaacgc ctccggcatt
     8881 gcccaacgta aatgcagcat caccgccgcc cgttgttacc agtgtaaggc tatctcctgc
     8941 tgccgggctg gcaccggtgt ccgtcacgaa tattttgaaa tcacctgttg cctgaccggt
     9001 gacgttgagc tgatcgctct gatgattcgc catatcggta cgcatataaa aattgccgtt
     9061 tcccgacagg gtattggtgg tcaacgtagt gaaattttcc gcacttgccg tcacagatga
     9121 acgagtcact ggctcatcca tcataatcac atttgcgcca gacatcgcaa ggttggctaa
     9181 gttaaggcgg gtgttttggt tcgtcgtagt gatatcggca atgatggtcc atgcgctatt
     9241 ttcgagcgaa acatccgctt tgctggtatc cacggcacca tgcgttctaa aagaagcgcc
     9301 ctcagaaacc actaccttac ccaccaacat agcgggtgca taatttattt gcggcttcat
     9361 gacctcaaga atgccatcat tccctctgac tgaaacgtta accattgtgc cagcccagac
     9421 attagcgcgg ccattgttga taaccatatt ttcagagatg gctttagagg aatacgttat
     9481 acttccattc tgatctgatc gaccgagatc atataccgca tttgcctgca ctctcgtacc
     9541 agtatccttt cccagcgatt gcatagtggc atgctcatcg aggatagtgt caatggcctg
     9601 cgtgtcctcc ataacaataa gcccagaacc atcatccagt tcataatttt ttgacacacc
     9661 atcttttata ctaaattggc ctttactgtt tgtaccactc acttccagcg cattcgttga
     9721 gacaattaat tttccgcctg cttttttatc aacgccagtt gcagtcccgc catccataac
     9781 ctccagtaag ccgccactat ctacagtagt attataagcg aagtcatttt cttcaactcg
     9841 caaacttccg ccgttttcca acagcatatt gttagcaata ccattcttga tttcgaactg
     9901 accgagacgg tttgttccga atacctccat gacccgcgtg cttgctttaa tagcaccgcc
     9961 tgctttctga tctaccgcaa atgctaatcc cccgtcttta acctccagta atcctccctt
    10021 ttcgatagcg gcaaagacgg caatcccatc tttttcaaca attaagcgcc cgcctttaat
    10081 cgtgggatca ttcgctgcgc cataaacctg tagcacacca ttattgaggg tagtctggtt
    10141 tgccgcgcca cccatttgaa caatttgata accatcattt aatacggagt attctgcggt
    10201 gccattcatt tcaatgtact gatacccgcc gttaatttca gtattactac ttataccaaa
    10261 ttctttaata tgctgttctc cgccaggatt gattttcgta ttattggcgg tcccataaac
    10321 cagctggata tctttttcca ggacaacacc atcaactgtc tcaccatact caactgttga
    10381 tgccaatgct atgggtgaat ataacgctgt agccaccaga acagatattg cggaacgaca
    10441 aagagaaaca gaaccagatt gatgcattga gctttcatcc tatgaaatta attgctgtta
    10501 aaagcattgg gtacagaaaa tacccatagc tccatacccg gagtcagttt ttaaaaactg
    10561 tttaaagaaa tgcacaagta ttgtgattga ttttttagtt gtttttcttg atgagaagct
    10621 gatgcaaaat tccgtcttta taatgaaaat gatgccaaag cgaacggcaa ggttgtagtt
    10681 ttcactacat gtccatacat aaaatggggt aacattcacg cgcctggtag cgataccaac
    10741 gctacgctca aacataatga ttctaataaa acctcaggag actactatgc ctgaagcaac
    10801 gccttttcag gtgatgattg tggatgatca tccacttatg cgacgcggtg ttcgtcagtt
    10861 actggagctt gatcctggct ttgaagtggt cgccgaagcg ggcgacggcg cgagtgctat
    10921 cgatctggct aatagactgg atatcgacgt gatcttgctg gatctcaata tgaaaggtat
    10981 gagtggcctg gataccctca atgccttgcg cagggatggc gttaccgcgc aaattattat
    11041 cctgaccgta tcagatgcct ccagcgatgt ctttgcgctg atagacgcag gcgcagacgg
    11101 ttatctgttg aaagacagcg acccggaagt gttgctggaa gcgattcgtg ccggagcgaa
    11161 aggcagcaaa gtctttagcg aacgcgtcaa tcagtactta catgaacgtg aaatgtttgg
    11221 cgcggaagaa gatcccttca gcgtgctgac ggagcgcgag ctggatgttc tgcacgagct
    11281 ggcacagggg ctgtcaaata aacagattgc ctcggtgttg aatatttccg agcagacagt
    11341 aaaagtacat attcgcaatc tgctgcgcaa actcaatgtc cgctcacgcg tggcggccac
    11401 cattctgttc ctgcaacaac gcggggcaca ataaaaatag cccgatggat ttatcatcgg
    11461 gctgagattt atgacaaacg caaaactgcc tgatgcgcta cgcttatcag gcctacgcgg
    11521 atcgatcaat ttattgaatt cacacgattg tgtaggccgg ataaggcgtt cacgccgcat
    11581 ccggcataaa ctaagcgcac tttgtcatca gtctggcctg atggaatcac catcgggcca
    11641 ctttttattt actctcctgc ggcgacaaat gttgcatcgc ctgcgcgata ctacgttcaa
    11701 tcaccgcacg gcgagtatcg ttggcaggta agagtttcaa catcatctcc cacgcggcaa
    11761 cggcttcgcc aaatcgctgc tgctcaaagg cattaaacgc atacatgctt aacacacgga
    11821 tattgctgtg gtcgcttctc accaactggc gcagcagttc gccgccgagg cggttgtcgt
    11881 tgggatcaga tgaacgtgtc aacgcttcag cgtatcccag tgcggcatcg ctgtttttcg
    11941 gatcgaggcg atacgcagta gcgtatgcat cggtggcgat actggcgttc cccagcgcca
    12001 tgccaacgcg gcccaacata atccaacctt ctatatctcc cggatttttt tgcagttgag
    12061 tacgcatccc cagcgcaagg cgcgacatct cttcttcgtt gagcggatcg gctttcggat
    12121 ccagcgccct gtccagcaac gccggagcct gtgccgtggc ctgctgccag attttcacct
    12181 gctgataatt gccagtctgg tagtagctga cgccagccac aattaacgcc accacaatac
    12241 ccggcagata aacaacatat ccggcacgct taccttccgg cacgctttgt tcaggaaacg
    12301 cttccggcac cacgcgtacc cgacgccgcg agcgggcgta tatgacccaa ccgccaatgc
    12361 caatagccac tacgggaagc acccacagca gcacggtcag cggcgttaac ggcggatcgt
    12421 aagtgacgaa gttgccgtaa cgcgccacca tataatagac aatctctttc ttacttttgc
    12481 cttcctgcat cagttcatac actttctgac gcaggtcggt ggcaatcatc gagttggaat
    12541 cggcaatgct gttgttctgg catttcgggc aacgcagttc ttcagtgagc tgacggaact
    12601 gttgttcctg tgcttcatct ttaaactgca acacatcgat ggtcgccagc gctgagccgg
    12661 agatcatcag catcagcacg cccaataaaa acctcattgt gcggcctcct tactgtattt
    12721 ctcccacagc ggcttgatct cttcttccca gacgcgagga ttcagatcgc ccgcatggcg
    12781 atagcgaatg ataccgttgc cgtcaataag aaacgtttct ggcgcgccat agacaccgag
    12841 atccagccct aacatgccgt cgccatcaaa caggcttagc gcgtaaggat tgcccagctc
    12901 tttcagccag ctgattgcct tctggcgatc gtctttatag ttcatgccga ccacgcggat
    12961 gccctgcgca gaaagctgat tcagatattg atgttccgca cggcaggtcg gacaccaggt
    13021 cgcccagacg ttaagcagca caggtttgcc ctgagtcagc acatccgcct gataaaactg
    13081 ccccggattg tccagtgatt cgagacgaaa cttcggcaca ggcttgccaa tgagcgccga
    13141 ttccagattg gtcggatcat ccccttcggc attacgcgcc agctgccaca gcagcgccgc
    13201 ggcaatcgcc aggaagataa tcaacggaat taacaatact ttgcgcttca tacagcctcc
    13261 ggcgcagttt tttgcgggct cacgcgctta cgatagcgag gatcaaacag acacagcagt
    13321 ccgcccaacg ccatcatcag cccgcccgcc caaatccagc gaacaaacgg tttgtagtaa
    13381 agacgcacag cccacgcgcc gttttccagc tcttcaccga gcgcggcgta caggtcacgc
    13441 gtgatgccgc cgtcaattgc cgcttcggtc atcatcgacc cggcagtgtt gtaataacgt
    13501 ttttccgcat acagcaccgt ttccggttta ccctctcgcg ttacgccaat agtcgccaca
    13561 ccgccgcgcc agttcgggcc agtcacctct ttgacatcac ggaaggtgaa gcgatattca
    13621 tgaatatcga cgctatcgcc ggacttcatg cgcacatcac gctcaacgct ataattctgg
    13681 ctaaaggcaa tgccaacaat tgtcactgcc agccccaggt gagccgccac catcccccaa
    13741 taactgaagg tggttttcgt gccgcgtgaa atacgtagcg cagcttccgc aattgccagc
    13801 accgcaatcc agcaggccat tgccaggccg agcaccgtca tcgccaccac tttgctttcg
    13861 aacagccacg gcaacagcag agacagcacc agcgttgaga tgaaggcgat aatcaataaa
    13921 ttgcggattt tacgtgggcg atcgcgcccc cagcgcacca gaggaccgac accaagcagc
    13981 agcgcaaacg gcaccatcag ccaggtaaac atggtgttga agaacggttc gccaatcgaa
    14041 atactgccca gtcccagttg cttgtgcacc agcggcagca gcgtccccag taataccacc
    14101 agcatcgcgg cgaccagcaa aacgttgttc gctaacagca gagattcccg cgaccacagc
    14161 gcattgttta cgcgtgagcg aactttgtgt ccacgcgcgg caaacagcag cagcgaacca
    14221 ccaatcacca gtaccataaa ggcgaggata aacataccgc gtgccggatc agaggcaaac
    14281 gcgtgtaccg ataccagcac gccggaacgc accaggaaag tccctagcag acacaacgag
    14341 aaggcactga ttgccagcag taatgtccac gctttgaagc tggcgcgttg ttcagtgacc
    14401 gccagtgagt gcatcagcgc agtccccacc agccacggca taaacgaggc gttttctacc
    14461 ggatcccaga accaccagcc accccagccg agttcgtaat aggcccatgc ggaaccgagc
    14521 acaatgccga gcgtcaggaa aatccacgcc gccagcgtcc acgggcgagt aaaacgcgca
    14581 taagtgctgt ccagacgccc gctcagcaaa gaagcaatgg caaaagcaaa cgccaccgag
    14641 aaacccacgt accccatata gagcagaggc ggatggaaga tcagccccgg atcctgtaac
    14701 agcgggttaa gatcgcgccc ttcaatcggg aagttcggca gcgtgcgtga gaatgggtta
    14761 gaggtaaaga gaatgaacag caaaaagccg acgctgacca tccccattat cgccagtacg
    14821 cgggccacaa tatccagcgg aatacgctga ctaaaaatcg ccaccgcaaa ggtccagccg
    14881 ctcatcagca gcacccacag cagcagtgag ccttcatgtg cgccccaggt agccgccacg
    14941 cgataccaca ccggaagctg ggtattggag ttgctggcaa cataggtgac ggtaaagtcg
    15001 ttgaccacga aagcattgac cagcaccaga aatgcgccag ccacagacat aaacagcagc
    15061 caggcaaaca agcgggaaga cgccatcatg cgcgcatctc cgcgcgccac gccccatagc
    15121 ggatacacgg acagcagcag cgcaatccct aacgccaggc acagcagccc gtttccaatt
    15181 tccggcatca tgatgctggg tccttataaa cactcgccgg gcgacggtgg ttagcttcca
    15241 tcgctttctc aacttctggc ggcgtgtagt tttcgtcgtg tttcgccagc acttctttcg
    15301 cgaggatatg attgcctttt tccagctcgc cctgcaccac aacgccctgc ccttcacgga
    15361 acagatccgg caaaatgcct tcgtaagaga catccactga gccttcagcg tcgtaaatgg
    15421 tgaaggtcac tttcagcgaa tttggatcgc gctgcacact acccggcatc accatcccgc
    15481 caacgcgcag acgctgaccg acttccggca tttgctgagt ttcacgcttg ccgtagagaa
    15541 tttcccccgg cgtataaaag agatcgatat tcgagcgcag cgcatatagc accagaccga
    15601 tagtcagcac cagccctgcc aacacggcac aggcaatcca caagcggttt ttacggcgaa
    15661 tattcatgca gcctcctgct gttgcgcagc acgtaaacgc gcctcacgcg cccgctgttg
    15721 cgccacgcca cgcagaattg cgcgatgttg catcaccgag tgcacgacca aaaccaccag
    15781 cggaataacg gtcatcacca ccgccagcca gacaaaaaag gcataaccgc ccattgcgaa
    15841 aaattcattc caggaagcaa atgcaggggt catttacggc ctcttttcag tatcagttca
    15901 ctcacccacg gacggcgttt ttccatcagc aaaatcaaat tacgcatccg catcagcgtc
    15961 agcgtggcag acaggagcag gaagccaaaa atcgaccagc gcagcggcga acgcatcgcc
    16021 ggatcgatac tttgctgcat ccgcgttgat ccctgatgca gggtgttcca ccactccacg
    16081 gagtaatgaa taatcggcag attcaccacg ccaatcagca ccaggatacc tgccgcacgg
    16141 cccgccagac ggcggtcgtc gaaggcgtgc cacagggcaa tcacaccaac atacagaaac
    16201 agcagcacca gttcagaagt cagacgtgca tcccataccc accaggtgcc ccacatcggt
    16261 tttccccatg cagagccggt aaccagggca ataaaggtaa acacggcacc aatgggggcc
    16321 atcgccgcca ccgccaggtt ggccattttc atctgccaga caaggccaat aaacgctgcc
    16381 actgccattg atgcataaat gcccatcgac cagatcgccg caggcacatg caggtagata
    16441 atgcggtagc tatttccctg ctgataatca gccggagcaa agccgaatcc ccagatccag
    16501 ccgacggtaa gcacgaccac actggcaatt gccagccacg gtataaacca gccacagatt
    16561 tgatacagcc gtggtgggat cgccagttga tgcagtgttt tccacatagt ttcgatacca
    16621 gactcgaaca aaaatcagta atccagcgtt attgaatgct gattcgtaac gctgccgccg
    16681 tcgcaaaagg acttaatgtc gcggtgcctg ccagcaacgc gcctaaaatt gccagatacc
    16741 cgtcaacagg caaatgcatg gaagccgcgt ccatcgcggc ggtggcaaag atgagtaatg
    16801 ggatagtcag cggtaacacc agtatgctga gcagcacacc accgcgctta agtcccacag
    16861 tcagcgccac gcccggtgca ccgagaaagc caagcgtagg cgttcccagc agcagcgtca
    16921 gcgccatcac ttgccagcca taaacatcca ttcccagtag cattgctacc agtggcgaaa
    16981 ggatgagtaa cggcagaccg gttaccatcc agtgcgccat caccttcgcc agcacaacgg
    17041 cgggcaaggg taacggcaac aacatcaatt gttcaagact gccgtcctgc aaatcgtcac
    17101 ggaacagtcg ttccagcgcc agcaaggatg aaagcagcgc tgcaacccag ataatgcccg
    17161 gtgcaatacg cgccagcagt tgcggctccg gaccgatact gagcggaaaa agggtaatta
    17221 caatcaggaa gaaccacagc gggttggcga tttcggcgct atggcgaaac gctacgcgca
    17281 gctcaagacg gaaaatgcgc cagaacatca tgcggccctc gtttgcgtca gtgaaatgcg
    17341 gcgaatttta ctttcagcaa cgttgagcgg ctggtgggta gtcagaatca caatcccccc
    17401 ctgctccgta tgctgcgcca tacgctgggt cagacgatcg acgccgttga cgtcaatcgc
    17461 ggtaaaaggc tcgtcgagga tccataacgt ggcacgggcc agccacagac gcgctaaagc
    17521 aacgcggcgt tgttgcccgg cagaaagttg atttaccgga atatcttcga atccggcaag
    17581 cccggcctgc gccagggctt ccagacattg tgcggtatcg ccatcgcgat gataaaagtg
    17641 cagattttct aacgccgtca gccgggtttt gatccccggc tgatggccta tccataacag
    17701 gttttgatgg tagctgtcgc gtacctgatg caagggctgc ccttgccaga gaacctcgcc
    17761 agcgtcaggg cgagacaacc ccgtcagcaa acggagaagc gttgtcttcc ccgcgccgtt
    17821 gctaccggtg atttgtaccc actctcctgc gttcagcgta aatgacaagc cactaaataa
    17881 ggttcgttca tcccgctcac aaagtaactc tctggcttca agcataccca cgcaataacc
    17941 ctgttaaaaa cctggctcga cttcacgcat atcgggcagc ttgtgcgcta tccctttatg
    18001 gcaatcaata caggtttgcc catctttcac cgcctggtca tgcatcttcg cggcaaccga
    18061 tttctgggcg gttgtatcca tatactcgaa gttgtgacag ttacggcact cctgcgagtt
    18121 attgtccttc atgcgccgcc actcattctg tgccatcgtc agacgatgag cttcaaattt
    18181 ctgcggcgtg tcaataacgc caaaaatttt accatacagc tctttacttg ctttgagctt
    18241 gcgtatcatc ttcggcacaa actcgtgcgg aacgtgacaa tccggacagg tcgcacggac
    18301 gccgctacgg ttgttgtagt gcacggaatc catgtattcc tgatacaccg tgttgcgcat
    18361 ttcgtggcag ctaatgcaga actcttcggt attggctttt tccatcccgg tgttaaaacc
    18421 accccagaag acgatgccgc caacaaaacc gatcaacagc agcgtcccca gcgccagacg
    18481 gctgggggta cgccaccatt tccacaggcg cttaatcaga ccaggcttac ggtcagaatt
    18541 tcccataata acctcttatt tcccgtaacc ttttgatggg gtaaaggtat tccccacgat
    18601 tggcgcggta tcggcctgcg gtacgtgaca ttgcagacag aaataacgac gcggagccac
    18661 ttctgcgccc actttgccgt cgctgtccat aaagtgagta ggactgatac gcggcgcgcc
    18721 agtggtgcga tagctttcga caccgtggca ctgcaagcag cggttggtat tggtcgttac
    18781 ctgataacct tcaacgctat gcgggatcat tggcggctga ttcacatagt tcagcggcat
    18841 ccgatcctgc tcttttggca tccgaatggc cccttcctgc gtcccggata cttccggcga
    18901 ctggctaaaa tcgacgccat tagccgccca aaccgcgccg cttaccacca gggccagcat
    18961 cgccgtccat tgacacagcg ctttcttcag gtcatggctt ttcatgattt cgctcccgaa
    19021 ctccatcgtg tagttattgt aaatacatcc tcagaacaga catccacgca gcgaccgcac
    19081 gtcatgcaat cgcggctggt gacctgcacc gggctttgtt catccagcac cggggcacgt
    19141 agcacatgcg gttccgggca aacatgaaaa caatccatac agcggttaca tttctgccga
    19201 tcgctggccg caacggtaat cacacctttg ctacccagca cgccatacag cgcgccaacc
    19261 ggacaaatgt gcccgcacca gccgtgttca acgaccagta gatcaaataa aaacagcgcg
    19321 agaataagca gcgcgccgct gccgaagccc attaccaggc tacgccccat cagggaaacg
    19381 gggttgatcc attcccagat gagcgtgcca gttaaggccg atcccaccag aatgaccacc
    19441 aacagcacgt agcgaatgtg gcgagggatc gtcgcagact gattgaggtc aaagcgcctg
    19501 cgtaaccagt tcgctaagtc ggtaatcggg ttcagcgggc agacccagct gcaaaataat
    19561 cgctttcccg ccagggcgta gagcacggtg ataatcaccg caccggtcaa cgccaccgtg
    19621 gcaggcagat gaccactggc aagactttgc agcgtcatca gcggatcggt cagcggaacg
    19681 gtgtcgaata acaggctact gctgtagttg ccgtgcaaga tccacacacc aaaccacgga
    19741 ccgctcaaaa acatccccag cacgaagaac tgacaaaggc gacgtaacac cagccaacgg
    19801 tgactgcgcc accagccttt tttctccagc gcctcgcgcc cggcgtcacg tttacgattt
    19861 gccattgttc ccctccagcc agccgaagcg gtaatggtga cctaactccc ctttcgccag
    19921 tgacaacggt aacaccttga ttgccggttg ttccagcacg cacacttttt cgcacttacc
    19981 gcagccggta caggcgtcgc tgtgaactgt cggcagaaag cgggcgtgct taccggtacg
    20041 cgtgttgcgc tccagctcca gggtgatggc ctcatcaatt ttcgggcatt cgcgataaca
    20101 aacatcgcag cgcagcccct gaaagttgag acagttttcc tggtctacca gtaccgccaa
    20161 gcccatccgc gcgtcgtcga tcgattcgat ctcacgatcc agcgcaccgc tcgggcacac
    20221 tttggcgcac ggaatgtcct cacacatttc gcaaggaata tcccgtgcga caaaatatgg
    20281 cgtgcccgcc gacagaccag aggccagcgt cgccagtttt aaggtgtcgt aagggcaagc
    20341 ctgaacacac tgaccacaac gcacacaggc actggcaaag gcgttctcgt ttatggctcc
    20401 gggcgggcgc aaccgcacgc cagatgcgcg tgcggtttgc tgttgtaacc ccagcgccac
    20461 acccacggca gccagcccgc ctgctgtgcg aacgacatcg cgcagaaagc ggcggcgacc
    20521 attttgaggt ttcgctgacc gggacataat ggcttacacc ttctccagtt tgaccgcgca
    20581 cttcttgaag tccgtctctt tcgagagcgg atcggtcgcg tccagcgtca gtttgttaac
    20641 cagctgtgcg gcgtcgaaga acggcatgta caccaggccc tgtggcggac ggttacgacc
    20701 gcgcgtttca acaatcgaga tgacttcgcc acggcgagaa accactttca ctttgtcgcc
    20761 acggcgcaga tcgcgcgctt tcgcatccag cgggtgaata aacaggaccg cttccgggaa
    20821 ggcgcggtgc agttccggca cacggcgagt catactgccg gtgtgccagt gctccaggac
    20881 gcgtccggta gagagccaca ggtcgtactc ttcatccggt gcttccgccg caggttcgaa
    20941 cggcagtgcg aagatcaccg ctttgccatc cggtttaccg tagaacttat agccttcgcc
    21001 cgctttcacg tacgggtcgt taccttcgct gtaacgccac tgcgtttctt taccgttcac
    21061 caccggccag cgcagaccgc gtgctttgtg gtagtcatcg aacggtgcca gatcgtgacc
    21121 gtgaccgcga ccaaaccatg cgtactcttc gaacagccct ttttgcagat agaagcccag
    21181 ctcgcgggat tcatcgttca gctgatcttc cgccagttcg gataccggga atttgctcac
    21241 ttcgggggtg gcatacagaa cttcgtacag cgttttgcca cgcagttccg gtttcttcgc
    21301 cagcagctct tccggccata cttcttcagt tttgaagcgg cgggagaact ggactaactg
    21361 ccagagatcc gatttcgctt cgcccggtgc ctgtacctgc tgacgccaga actgagtacg
    21421 gcgttcggcg ttaccgtaag cgccctcttt ctctacccac attgcggtcg gcaggatcaa
    21481 gtcggcggcc agcgcactga ctgtcggata cggatcggag acgatgatga agttgcgcgg
    21541 atcgcgccag cccggcatac gctcttcatt aatgttcggc ccggcctgca tgttgttggt
    21601 acacatggtc cagtaaacat tcagcttgcc gtctttcagc gcacggtctt gtgccaccgc
    21661 gtgcagaccg attttcgccg gaatggtacc gctcgggata ttccacttct tctcgcagat
    21721 atcacgatgt ttctcgttgg tcaccaccat gtccgcaggc agacggtgag caaaggtgcc
    21781 cacttcacgc gctgtaccac acgcagaagg ctgcccggtc agggagaacg gaccgcaacc
    21841 cggctgggaa attttgccgg tcagcaggtg caggttgtag accaggttgt tggcccacac
    21901 gccacgggta tgctggttga agcccatcgt ccagtaggag atgactttct tgttcggatc
    21961 ggcatacagc tgcgccagtt gttccagctg atcttttggt acgccagtca tttcagcggt
    22021 tttttccagc gtatactcag caacgaaggc tttgtaatct tcaaagctca tcggttcgga
    22081 ggcgtcagaa cccggattct tcgctgcttt ttccagcgga tgggtcggac gtaaaccgta
    22141 gccgatgtcc gtcgcccctt tgcgcaggtt aacgtgtttg ctaaagaagt cctgatttat
    22201 cgcattgttt tgaatgatat agttggcgat gtagttcagg atcaccaggt cagattgcgg
    22261 cgtaaagatg atgccgttat ccgccagctc gaagctacga tgctggtagg ttgaaagcac
    22321 cgccacggtg acgtcctggt tagagagacg acggttagtg atgcgtgacc agaggatcgg
    22381 gtgcatctcc gccatgtttg cgccccacag cacaaacgcg tcagcctgct cgatgtcgtc
    22441 atagcagccc atcggctcat ccataccaaa ggtacgcata aagccaacta ctgccgacgc
    22501 catacagtga cgcgcgttcg ggtcgatgtt gttcgaacgg aagcccgctt taaacagctt
    22561 ggacgcggca taaccttccc agatagtcca ctgaccagaa ccgaacatac cgatcgattc
    22621 cggccctttt tctttcaggg cggttttgaa tttctcttcc atcacatcaa aggcttgatc
    22681 ccaggtgatt ggggtaaatt cgccttcttt gtcatattta ccgtttttca tacgcagcag
    22741 cggctgcgtc aaacggtctt taccgtacat gatcttgggc aggaaatagc ccttaatgca
    22801 gttcaggcca cggttaaccg gtgcgtccgg gtcgccctga caggccacca cacgcccctg
    22861 ctgcgttccg accagaacgc cgcaaccagt accgcagaaa cggcacggcg ctttgtccca
    22921 tttaatggct tcctgctgac caacaacggc gcgggcaacg cccggcacgc tgagaccggc
    22981 agccgccgca gcggccgcaa cggcgttagc tttcataaag ctacgacgac tgagtttcat
    23041 ggtgtttcct caccttgctc ttcctgctgg tgataaacca gcgacaccgc cagcacgccc
    23101 tctacgttgc gtactgactc aatggtttgg atcagcgttt cgctgtcttc tgcttccacc
    23161 accacaatca actgaccgct cggcgcgtcg ctgacagcaa cttcacagcc gggaaaggcg
    23221 ttcagttggg tgctgatgtc tgaaattcgt tcgcttttgg cctgcacgac caggctgcaa
    23281 acttgccagt tagtgtgcat ggagatactc cgcagttatg gctgataccg gacagctggc
    23341 tgcacatgct ccacatccgt tgcaaagttg gctattaagt tgcggctggt agatcccgga
    23401 gagcgtcggg cgaaagataa ttgccattgg ctcacagcta tcctgacagc ggcggcattc
    23461 aactgactga tacgccaggc acgcgtcccc aatggtgaac tgcaaatccc aggccctggt
    23521 gtggcgcgga gaaaatagcg attcggggca agcctgcgcg caggcgtaac agaagctgca
    23581 ctcgttattt ttgaaattaa cgctcggata gccgcccgcg ccgcgttgca gaatgttgtt
    23641 ttcacaagca ttaatacagg cgtcgcagcg ggtgcaatgg gtcagaaaat gagattcatc
    23701 accggaccag ggcggacgga taccgttact ggctttgcgc cagcgaccag tgagtatgcc
    23761 ccgacgggat gcatcaatct tcacattgac cttccatcat taacgcgctc tgaaaattga
    23821 gagcgaccaa ataaaccgca taattaataa gccattttta tagccgctaa gatattaaag
    23881 gatgtgtcaa agatgcatac cccgatcgag gtaaaacctg tagcaggatc aaaagagtgg
    23941 cgggaagcgt ggcaaaaacg gg
//
LOCUS       DAPGOF010000068        23904 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.068, whole genome shotgun sequence.
ACCESSION   DAPGOF010000068 DAPGOF010000000
VERSION     DAPGOF010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23904
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.068"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            26..3778
                     /gene="yfaL"
                     /locus_tag="RHV13_002334"
     CDS             26..3778
                     /gene="yfaL"
                     /locus_tag="RHV13_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HDV1241861.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQVWSIADGQWLVFSDMINNASGGAVFLQQGAEFTLSPENETGMTLFAN
                     NTVSGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAIDLRVTNA
                     VFRNNIANDGKGGAIYTINNDIYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSSAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQN
                     QAELNVGSTQQTFVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLAGAQSMALTGDIVVDAGAVLSLEGDAADLAALQDDPQSIVLNGGMLDLSDFSTWQS
                     GTSYKDGLEVSGSSGTVIGSQDVVDLAGGNDMHIGGDGKDGVYVVIDAGDGQVSLAND
                     NQYLGTTQIASGTLMVSDNSQLGDTHYNRQVLFTDKPQESVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADILPYASSLWVGDGATFVTGADQDIQSIDAISSGTIDISDGTVLR
                     LTGQDTSVDLDASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLADVTVNGDLTNT
                     SGAVSLQNGVVGDTLTVNGDYTGGGTLLFDSELNGDDSVSDQLVMNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPSPPEPDPTPDPDPTPDPDPTPDPAPTPAYQPVLNAKVGGYLNNLRAANQAF
                     MMERRDHAGGDGQTLNLRVIGGRYHYTAAGQLAQQEDTSTVQLSGGLFSGRWGTDGEW
                     MLGIVGGYSDNQGDSRSSMTGTRADNQNHGYAVGLTSSWFQHGKQKQGAWLDNWLQYA
                     WFSNDVSEHEDGVDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAA
                     NRARVSQSQGDDIQTRLGLHSEWRTAVHVIPTLDLNYYHDPHSTEIEEDGSTISDDAV
                     KQRGEIKVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(3919..4641)
                     /locus_tag="RHV13_002335"
     CDS             complement(3919..4641)
                     /locus_tag="RHV13_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HDV1241862.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNTFKLGPGVDVNYMLHTQNK"
     gene            4788..7415
                     /gene="gyrA"
                     /locus_tag="RHV13_002336"
     CDS             4788..7415
                     /gene="gyrA"
                     /locus_tag="RHV13_002336"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HDV1241863.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7564..9252
                     /locus_tag="RHV13_002337"
     CDS             7564..9252
                     /locus_tag="RHV13_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HDV1241864.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKLNNETIPVYQLRYNGNNALMFATYQDKMVVFSSTDMLFK
                     DDQQDTEATAIASDLLSGKKRWETSFGLEERTAGKTPVRQRIVVSANLLGFGYQRLMP
                     SFAGVRFEMSNDGWHSFLALNDESASVDASFDFTPVWSSMPAGASFCVAVPYSHGIAE
                     EMLSQIGQEKDKLNGALDGAAGLCWYEDSKLQTPLFVGQFDGTAEQAQLLGKLFTQNI
                     GAHESKAPEGVLPVSQTQQGEAQIWRREVSSRYGQYPKAQAVQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            9249..9872
                     /locus_tag="RHV13_002338"
     CDS             9249..9872
                     /locus_tag="RHV13_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HDV1241865.1"
                     /translation="MRNGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            9896..14410
                     /locus_tag="RHV13_002339"
     CDS             9896..14410
                     /locus_tag="RHV13_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HDV1241866.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSHSRQNVTQALPELQLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGKQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGKELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVDVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFDKPGNYNLTLRDKDGLILAGLSHAVSGKGSM
                     SHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPGSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGDNALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKTEDFSEEDPRDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQAAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGEGEYWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKTAQRSNIPVTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            14411..16060
                     /locus_tag="RHV13_002340"
     CDS             14411..16060
                     /locus_tag="RHV13_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HDV1241867.1"
                     /translation="MNWRRIVWLLALVTQPTLAEETPLQLALRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFANGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQAMAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            16065..16841
                     /locus_tag="RHV13_002341"
     CDS             16065..16841
                     /locus_tag="RHV13_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HDV1241868.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPVEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGAGVIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            17116..19965
                     /gene="rcsC"
                     /locus_tag="RHV13_002342"
     CDS             17116..19965
                     /gene="rcsC"
                     /locus_tag="RHV13_002342"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HDV1241869.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLESSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(20147..20797)
                     /gene="rcsB"
                     /locus_tag="RHV13_002343"
     CDS             complement(20147..20797)
                     /gene="rcsB"
                     /locus_tag="RHV13_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HDV1241870.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(20814..23486)
                     /gene="rcsD"
                     /locus_tag="RHV13_002344"
     CDS             complement(20814..23486)
                     /gene="rcsD"
                     /locus_tag="RHV13_002344"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HDV1241871.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         5816 a         5825 c         6529 g         5734 t
ORIGIN      
        1 tatataaaat gatggtcaga ttaatatgcg gattatcttt ctacgcaagg agtatttatc
       61 tctactcccg tcaatgattg catctctttt ctctgctaac ggtgtcgcgg cggccattga
      121 tttatgccag ggatatgata tcaaagcgag ttgtcacgcc agcaggcaaa gcctttcagg
      181 cattacgcag gtctggagta tcgccgatgg gcaatggctg gttttttcgg atatgatcaa
      241 taatgccagc ggtggggccg tatttttgca acaaggagcg gaatttacat tatcaccaga
      301 aaatgaaact ggaatgactc tgtttgccaa taacaccgtt tcaggagaat ataataacgg
      361 cggggcaata tttgctaaag aaaactcaac gctgaatctt acggatgtta ttttttctgg
      421 taacgtcgca ggcggctatg gtggcgcaat ctattcttct ggtactaacg ataccggtgc
      481 catcgattta cgtgtcacta acgccgtgtt tcgcaataac atcgctaatg acggcaaagg
      541 tggtgcaatt tataccatca ataatgatat ctatttaagt gatgatgttt ttaacaataa
      601 ccaggcatat acatcaacaa gttacagtga tggcgatggc ggcgcaatcg atgtcacaga
      661 taataatagc gacagcaagc atccttcagg ttatacgata ataaataaca ctgcctttac
      721 aaataacact gccgaaggtt atggcggggc gatatatacc aatagcgcga cggctcccta
      781 tcttattgat atttctgttg atgacagcta cagccagaac ggaggcgtgt tagtcgatga
      841 gaacaatagc gcagcaggct atggagatgg tccttcctct gcggcgggtg gctttatgta
      901 tctcggctta agtgaagtta catttgatat tgccgacgga aaaacgctgg ttattggcaa
      961 tacagagaat gacggagctg ttgactctat tgctggtacc gggttaatca ccaaaacagg
     1021 ttccggcgat ctggtactta atgcagataa caatgacttt actggtgaga tgcagattga
     1081 aaacggtgaa gttaccctgg gccgcagcaa ctccctgatg aatgtcggcg atacgcattg
     1141 ccaggacgat ccgcaagact gctacggtct gacgataggg agtattgata agtaccagaa
     1201 tcaggcagag ctaaatgttg gctccaccca acaaaccttt gtgcactcat tgacgggctt
     1261 tcagaatggc actttaaata tcgatgctgg tggcaatgtt actgttaatc aaggcagttt
     1321 tgctggcacc atcgaaggtg ctggtcagct caccattgcg caaaacggca gctatgtgct
     1381 ggcgggggcg cagtcgatgg cgctgaccgg cgatatagtg gtggatgctg gtgcggtgct
     1441 ttcgctggaa ggcgacgcgg cagatcttgc cgctctccag gacgatccgc agtcgatcgt
     1501 gttaaacggc ggtatgctcg atctctctga tttctccacc tggcagagcg gtacatcata
     1561 caaagatggc cttgaagtca gtggcagcag cggaacggtt atcggcagtc aggatgtggt
     1621 agatcttgca ggcggaaacg atatgcatat cggcggcgac gggaaagatg gcgtctacgt
     1681 ggtgatcgat gcgggtgacg ggcaggtcag cctggcaaat gacaatcaat acctcggcac
     1741 aacgcaaatc gcttccggta cgctgatggt gagcgacaat tcgcagcttg gagataccca
     1801 ttataaccgc caggttctct ttaccgataa gccacaagaa agcgtgatgg agattactgc
     1861 caatgtcgat actcgctcta caacgactga gcatgggcgt gatattgaaa tgcgcgccga
     1921 cggtgaagtg gcagttgatg cgggggtaga cacgcagtgg ggcgcactga tggctgacag
     1981 cagcgggcag catcaggatg agggtagcac attgacgaaa acgggggcgg gtacgctgga
     2041 gctgaccgcc agcggtacaa cgcagtcggc ggtacgtgtc gaagaaggca ccctgaaagg
     2101 tgatgttgct gatatccttc cttatgcttc gtcactgtgg gttggtgatg gggcaacgtt
     2161 cgttactggc gcggatcagg atattcagtc aattgatgct atttccagcg gcactatcga
     2221 catcagcgat ggtacggttt tgcgcctgac cgggcaggat acttccgtcg accttgatgc
     2281 ctcactgttt aacggcgatg ggacgctggt gaatgccacc gatggcgtga cgttgacagg
     2341 tgagcttaat accaaccttg aaactgacag cctgacctat cttgccgacg tgacggttaa
     2401 tggtgatctg accaatacgt ccggtgcggt tagcctgcaa aatggcgtcg ttggcgatac
     2461 gctgacggta aacggtgatt ataccggcgg cggtacgcta ctgttcgaca gcgaattaaa
     2521 cggcgatgac tcggtaagcg atcaattggt gatgaacggt aatactgctg gcaacacaac
     2581 tgtggtggtt aactccatta cagggattgg tgagccgaca tcgacaggca ttaaagtggt
     2641 tgatttcgca gctgatccca cgcagtttca aaacaatgca cagttcagtc tggctggcag
     2701 tggctacgtc aacatgggag cgtatgacta cacgctagtg gaagataaca acgactggta
     2761 tctgcgatcg caagaagtaa cgccgccatc gccacctgag ccagacccga ctcccgatcc
     2821 tgatcccacg ccggatcctg acccaacccc cgacccggcc cctacgcctg cttaccagcc
     2881 ggtgctgaat gccaaagttg gcggttatct caataacctg cgggcggcaa atcaggcgtt
     2941 tatgatggag cgacgcgatc acgctggtgg cgatggtcag acgctgaatt tacgtgttat
     3001 cggcggacgt tatcattaca cggcggcagg gcaactggct cagcaggaag acacttctac
     3061 ggtgcagctt agcggcggct tgtttagcgg gcgctggggc acggatggcg agtggatgct
     3121 tgggattgtt ggtggctaca gcgataacca gggcgacagc cgctcgagta tgaccggaac
     3181 tcgcgccgat aaccagaacc acggttatgc ggttgggctg acctcaagct ggtttcagca
     3241 cggtaagcag aagcaggggg cctggctgga taactggttg cagtacgcgt ggtttagcaa
     3301 tgatgtttct gaacatgaag atggcgtgga tcattaccat tcgtcgggga ttatcgcctc
     3361 gctggaagcg gggtatcagt ggttaccggg gcgtggtgtg gtgattgaac cgcaggcgca
     3421 ggtgatttat cagggcgtgc agcaggatga ttttaccgcc gctaaccgtg cgcgcgtgtc
     3481 acaatcgcag ggtgatgata ttcagacgcg gctgggttta cacagcgaat ggcgtaccgc
     3541 tgttcatgtc ataccaacat tagatctgaa ttattatcac gatccccatt cgacggaaat
     3601 tgaagaggat ggcagcacta tcagtgacga tgcggtgaag caacggggtg aaataaaagt
     3661 gggagtcacg ggcaatatca gtcagcgagt gtccctgcgc ggcagcgtgg cgtggcagaa
     3721 agggagtgat gattttgccc agacggcagg gtttttgtcg atgacggtga aatggtaatc
     3781 gctgtctgac catcactatt gccggatgcg gcgtaaacgc cttatctggc ctacggagag
     3841 catttgtagg cctgataaga cgcggcaagc gtcgcatcag gcattggcgc ggttgccgga
     3901 tgaatccaaa caacaacctc acttattctg cgtgtgcaac atatagttca catccacacc
     3961 ggggccgagt ttaaaagtat tagtgatcgg gttgtaatgt agcccggtta tatgtcgctc
     4021 tttaaggctt gtctgatcta cccagccaag cagctctgcc ggtttaataa acttcttcac
     4081 atcatgcgtg cctttgggca ccatgcgcaa aatatattcc gcgccaacca ccgccatcag
     4141 ccatgacttg ccgttgcggt taagcgtcga gaaaaagaca tcgccgcctg gtttcaccag
     4201 ttgcgcacag gctctgacca ctgactgcgg atcgggtacg tgctccagca tctccatgca
     4261 ggtcaccaca tcatactgcc cggcatgttt tgccgcgtgc tcttccacgg tttcctgcac
     4321 gtaatccacc tgaatgccgc tttccagtgc gtgcagtttt gccacctgca atggctcaaa
     4381 tcccatatcc agaccggtca ccgtcgcgcc ttcgcgcgcc atactctcgg ccagaatgcc
     4441 gccgccacaa ccgacatcga gcaccttttt gccaaataaa ccgccagcac gctcggcaat
     4501 atagcccaga cgcagcgggt taatgcggtg cagcggtttg aactcacctt ccagatccca
     4561 ccagcgggag gcgacggctt caaatttagc gatctcttcg tggtctacgt tatggtttac
     4621 cggcgatttt tcggcattca ttggcacttc tactccgtaa ttggcaagac aaacgagtat
     4681 atcaggcatt ggatgtgaat aaagcgtata ggtttacctc aaactgcgcg gctgtgttat
     4741 aatttgcgac ctttgaatcc gggatacagt agagggatag cggttagatg agcgaccttg
     4801 cgagagaaat tacaccggtc aacattgagg aagagctgaa gagctcctat ctggattatg
     4861 cgatgtcggt cattgttggc cgtgcgctgc cagatgtccg agatggcctg aagccggtac
     4921 accgtcgcgt actttacgcc atgaacgtac taggcaatga ctggaacaaa gcctataaaa
     4981 aatctgcccg tgtcgttggt gacgtaatcg gtaaatacca tccccatggt gactcggcgg
     5041 tttatgacac gatcgtccgt atggcgcagc cattctcgct gcgttacatg ctggtagacg
     5101 gtcagggtaa cttcggttcc atcgacggcg actctgcggc ggcaatgcgt tatacggaaa
     5161 tccgtctggc gaaaattgcc catgaactga tggccgatct cgaaaaagag acggtcgatt
     5221 tcgttgataa ctatgacggc acggaaaaaa ttcccgacgt catgccaacc aaaattccta
     5281 acctgctggt gaacggttct tccggtatcg ccgtaggtat ggcaaccaac atcccgccgc
     5341 acaacctgac ggaagtcatc aacggttgtc tggcgtatat cgatgatgaa gacatcagca
     5401 ttgaagggct gatggaacac atcccggggc cggacttccc gacggcggca atcatcaacg
     5461 gtcgtcgcgg tatcgaagaa gcttaccgta ccggtcgcgg caaggtatat atccgcgccc
     5521 gtgctgaagt ggaagttgac gccaaaaccg ggcgtgaaac cattatcgtc cacgaaattc
     5581 cgtatcaggt gaacaaagcg cgcctgatcg agaaaattgc ggaactggta aaagaaaaac
     5641 gcgtggaagg catcagcgcg ctgcgtgacg agtctgacaa agacgggatg cgcatcgtga
     5701 tcgaagtgaa acgcgatgcg gtcggtgaag tggtgctcaa caacctctac tcccagaccc
     5761 agttgcaggt ttctttcggt atcaacatgg tggcattgca ccatggtcag ccgaagatca
     5821 tgaacctgaa agacatcatc gcggcgtttg tccgtcaccg ccgtgaagtg gtgacccgtc
     5881 gtactatttt cgaactgcgt aaagctcgcg atcgtgctca tatccttgaa gcattagccg
     5941 tggcgctggc gaacatcgac ccgatcatcg aactgatccg tcatgcgccg acgcctgcag
     6001 aagcgaaaac tgcgctggtg gctaatccgt ggcagctggg caacgttgcc gcgatgctcg
     6061 aacgtgctgg cgacgatgct gcgcgtccgg aatggctgga gccagagttc ggcgtgcgtg
     6121 atggtctgta ctacctgacc gaacagcaag ctcaggcgat tctggatctg cgtttgcaga
     6181 aactgaccgg ccttgagcac gaaaaactgc tcgacgaata caaagagctg ctggatcaga
     6241 tcgcggaact gttgcgtatt cttggtagcg ccgatcgttt gatggaagtg atccgtgaag
     6301 agctggagct ggttcgtgaa cagttcggtg acaaacgtcg tactgaaatc accgccaaca
     6361 gcgcagacat caacctggaa gatctgatca ctcaggaaga tgtggtagtg acgctctctc
     6421 accagggcta cgttaagtat cagccgcttt ctgaatacga agcgcagcgt cgtggcggga
     6481 aaggtaaatc tgccgcacgt attaaagaag aagactttat cgaccgactg ctggtggcga
     6541 acactcacga ccatattctg tgcttctcca gccgtggtcg cgtctattcg atgaaagttt
     6601 atcagttgcc ggaagccact cgtggcgcgc gcggtcgtcc gatcgtcaac ctgctgccgc
     6661 tggagcagga cgaacgtatc actgcgatcc tgccagtgac cgagtttgaa gaaggcgtga
     6721 aagtcttcat ggcgaccgct aacggtaccg tgaagaaaac tgtcctcacc gagttcaacc
     6781 gtctgcgtac cgccggtaaa gtggcgatca aactggttga cggcgatgag ctgatcggcg
     6841 ttgacctgac cagcggcgaa gacgaagtaa tgctgttctc cgctgaaggt aaagtggtgc
     6901 gctttaaaga gtcttctgtc cgtgcgatgg gctgcaacac caccggtgtt cgcggtattc
     6961 gcttaggtga aggcgataaa gtcgtctctc tgatcgtgcc tcgtggcgat ggcgcaatcc
     7021 tcaccgcaac gcaaaacggt tacggtaaac gtaccgcagt ggcggaatac ccaaccaagt
     7081 cgcgtgcgac gaaaggggtt atctccatta aggttactga acgtaatggt ttagtggttg
     7141 gcgcggtaca ggtagatgac tgcgaccaga tcatgatgat caccgatgcc ggtacgctgg
     7201 tacgtactcg cgtttcggaa atcagcatcg taggccgtaa cacccagggc gtgatcctca
     7261 tccgtactgc ggaagatgaa aacgtagtgg gtctgcaacg tgttgctgaa ccggttgacg
     7321 aggaagatct ggataccatc gatggcagtg ccgcggaagg ggacgatgaa atcgctccgg
     7381 aagtggacgt tgacgacgag ccagaagaag aataatttta cttcttcatg ccaaaaggga
     7441 gctatctccc ttgtttgaat tgaaaagtcc aggctgcaaa gtctgggctt ttgtcgtatt
     7501 agggcacggt aaagtttggc tgtgcccgta aaaaatggct ggctatacac aaggaatgtt
     7561 gcaatgagtg gtgaaaaaaa ggcgaaaggc tggcggttct atggtcttgt aggttttggc
     7621 gcaatagcac tgctttccgc tggcgtctgg gcgttgcaat atgctggcag tgggccagaa
     7681 aaaacgttgt cgccgctggt ggtgcacaac aatctgcaaa tcgatctcaa tgagccggac
     7741 ctctttctcg acagcgactc tctgagccag cttcccaaag atctcctcac cattccgttt
     7801 ctccacgatg ttctgagcga agatttcgtt ttctattatc agaatcatgc cgatcgtctg
     7861 ggcattgaag gcagcattcg tcgtattgtc tatgaacacg atctcacgct gaaagataag
     7921 ctcttttcgt cactcttaga tcagcccgcg caggcagcgc tgtggcacga taaacaaggc
     7981 catctttcac attatatggt gctgatccag cgtagtggct taagcaagct gctggagcca
     8041 ttgctgtttg ccgctaccag cgacagccag ttaagcaaaa cggaaatcag tagcatcaag
     8101 ctaaataatg aaaccattcc tgtttatcag ttgcgctata acggcaataa cgccctgatg
     8161 ttcgcgactt atcaggacaa gatggtggtt ttttccagta cggatatgct gtttaaagat
     8221 gaccagcagg ataccgaagc cacggcaatc gcaagtgatt tgttaagtgg taagaaacgc
     8281 tgggaaacca gttttggcct tgaagagcgt actgccggaa aaacaccagt acgccagcgc
     8341 atcgtggtca gtgcaaattt gctgggtttt ggctaccagc gattaatgcc ttcttttgcc
     8401 ggtgtgcgct tcgaaatgag caacgacggt tggcatagct ttctggcgtt aaatgatgaa
     8461 tccgctagtg tggatgccag tttcgatttt acgccggtat ggagcagtat gcctgccggg
     8521 gccagcttct gcgtggctgt gccttattca cacggtattg ctgaagagat gctctcacaa
     8581 atcggtcagg aaaaagataa gttgaatggg gcattagatg gcgcagccgg gttgtgttgg
     8641 tatgaggatt caaaactgca aaccccgcta tttgttggcc agtttgatgg cactgccgaa
     8701 caggcgcaat tgcttgggaa actgtttacg caaaatattg gcgcgcacga aagcaaagcg
     8761 ccagaaggtg ttttgccggt aagccagact cagcagggcg aagcgcaaat ctggcgtcgc
     8821 gaagtgagtt cccggtacgg tcagtatccg aaagcgcagg cggtgcaacc cgatcaatta
     8881 atgtcggatt attttttccg cgtgtcgctg gcgatgcaaa acaaaacgct gcttttctcc
     8941 ctcgatgaca cgctggttaa taacgccctg caaacactga ataaaacccg cccggcaatg
     9001 gtggatgtaa tacccactga tggcatcgtt ccgctctata tcaatccaca aggcgtggcg
     9061 aaactgctgc gtaacgaaac gttgaccagt ctgccgaaga atctcgaacc ggttttttat
     9121 aacgccgcac aaactttatt aatgccgaag ctggacgctt tatctcaaca accgcgttat
     9181 gtcatgaagc tggcccagat ggaacccggt gccgcctggc agtggctacc cataacctgg
     9241 cagccgctat gaggaacggg ctgctggcgc tgatttgctg gctgtgttgt gttgttgccc
     9301 atagcgaaat gctgaatgtc gaacaatccg ggctgtttcg cgcctggttt gtacgcattg
     9361 cacaagaaca gctccgccag gggccaagtc cacgctggta tcagcaggat tgtgcgggcc
     9421 tggtgcgatt tgcggcgaac gagacgctga aagttcacga cagtaaatgg ctaaaaagta
     9481 acggtttatc tagccagtat ttgccgccag agatgacgct aacacctgaa cagcgtcaac
     9541 tggcgcaaaa ctggaatcag gggaacggga aaaccggccc ctacgtgacc gcgattaatt
     9601 tgattcagta caacagccag tttattggcc aggacataaa ccaggcgctg cctggcgata
     9661 tgattttttt cgatcagggc gatgcccagc acttaatggt ctggatgggg cgttacgtca
     9721 tctaccacac cggaagcgcc acgaaaactg acaacggaat gcgcgcagtc agtctgcaac
     9781 aacttatgac atggaaggac acccgatgga tacccaacga ttccaatccc aatttcattg
     9841 gcatttatcg tttaaatttt ctggcgcgat agcagcgtgc ttgtccctgt ctcttgtggg
     9901 aacagggctt gctaatgctg atgattcgct tccttccagt aactatgcgc cgcccgcagg
     9961 gggaacattc tttttgcttg ctgacagcag ttttagcagc agtgaagagg cgaaagtgcg
    10021 actggaagcg ccggggcgtg attatcggcg ctatcagatg gaagagtacg gcggcgtgga
    10081 cgttcgcctg tatcgtattc ctgacccgat ggcatttttg cgccagcaga aaaacctgca
    10141 tcgcattgtg gtgcaaccgc aatatttggg cgacgggctg aacaatacgc taacctggct
    10201 gtgggataac tggtacggca aatctcgccg cgtgatgcag cgtactttct cttctcattc
    10261 acggcagaat gtgactcagg cgttacccga attacagctc ggcaatgcca ttattaaacc
    10321 ttcccgttat gtacagaaca accagttttc accgctgaaa aaatatccac tagtggaaca
    10381 gttccgttac ccattatggc aggctaaacc ggtcgagccg cagcaagggg taaaactgga
    10441 aggcgcatcc agcaatttca tctcgccgca gccgggtaac atctatattc cgctaggcaa
    10501 gcaagagccg ggactgtacc tcgtcgaggc gatggttggt gggtatcggg cgacgacggt
    10561 ggtgtttgtt tccgataccg tggcgcttag caaagtgtca ggcaaagagc ttctggtgtg
    10621 gaccgcgggt aaaaaacagg gtgaagcgaa gcccggctca gagatcttgt ggactgacgg
    10681 tcttggcgtg atgacccgcg gtgtgaccga tgacagcggt accttgcagt tacaacatat
    10741 atcgccagaa cgttcataca ttctgggtaa ggatgctgaa ggcggcgttt ttgtctccga
    10801 gaacttcttc tacgaaagcg aaatctacaa cacccgcttg tatattttta ccgatcgccc
    10861 gctatatcgc gcaggcgatc gtgtcgatgt taaagtgatg ggccgcgagt tccacgatcc
    10921 gttgcattca tcccccatcg tcagcgcccc ggcgaagctt tcggtgctgg acgctaacgg
    10981 cagtctgttg caaaccgtcg atgtcacgct ggatgcgcgc aatggcgggc agggaagttt
    11041 ccgcctgcca gaaaatgccg ttgccggagg ttatgagtta cgtcttgctt accgcaatca
    11101 ggtctatagc agcagttttc gcgtggcaaa ctacatcaag ccacatttcg agattggttt
    11161 agctctcgac aaaaaagagt tcaaaactgg cgaagcggtc agcggcaaac tgcaactgct
    11221 ctacccggat ggcgagccgg taaaaaatgc ccgcgtgcag ttaagtttgc gcgctcagca
    11281 attatcaatg gtcggtaacg atttgcgtta tgccggacgt ttccccgtgt cgctggaagg
    11341 cagtgaaacg gtgtccgacg ccagcggtca tgtggcgtta aatctccccg ccgccgataa
    11401 accgagccgc tatttgttaa ccgtctccgc cagtgacggc gcggcgtatc gcgtcaccac
    11461 caccaaagag atcctcattg aacgcggcct ggcgcattac tctttaagta ccgccgcaca
    11521 atacagtaat agcggcgagt cggttgtgtt ccgttatgcc gcgctggaat cttcaaaaca
    11581 ggttcctgtt acgtatgaat ggttgcgtct cgaagaccgc acgagccata gcggagagct
    11641 accgtctggc ggcaaatcct ttaccgtcaa tttcgataaa cctggcaact acaatctgac
    11701 gttacgcgat aaagacggct taattctcgc cgggttaagc catgcagtca gcggtaaggg
    11761 cagcatgtcg catactggta cggtagatat cgtggcagat aaaacgctgt accagcctgg
    11821 cgaaaccgcg aagatgctga ttaccttccc ggagccaatt gatgaagcat tattgacgct
    11881 ggaacgcgat cgcgttgaac agcagtcgct gctatcgcat ccggcaaact ggctgacgtt
    11941 acaacgttta aacgatactc agtatgaagc ccgtgttcca gtgagcaatt cctttgcgcc
    12001 taacatcact ttttcggtgc tgtatacccg taacggtcag tacagttttc agaacgccgg
    12061 gatcaaagtt gccgttcccc agctggatat ccgggtgaaa acggacaaaa cccattacca
    12121 gcctggtgaa ctggtcaatg tcgaattaac ctcgtcgctg aaaggtaaac ctgtttctgc
    12181 gcagctaacg gtaggcgtgg tcgatgaaat gatctacgcg ctgcaaccag aaatcgcgcc
    12241 gaatatcggc aaatttttct atccgctggg gcgtaacaat gtgcgtacca gctccagtct
    12301 gtcgtttatc agctacgacc aggcgctctc cagcgagccg gttgcgcctg gcgcgactaa
    12361 ccgcagcgag cggcgagtaa aaatgcttga acgtccacgg cgtgaagagg tggataccgc
    12421 ggcatggatg ccgtcactca caaccgataa acaaggcaaa gcgtatttca cgttcctgat
    12481 gcctggttcg ttaacccgct ggcgtatcac cgcgcgtggg atgaacggcg acgggctggt
    12541 cgggcagggg cgtgcttatc tgcgttcgga aaaaaatctc tacatgaagt ggagtatgcc
    12601 aacggtgtat cgcgtgggcg acaaaccggc ggcaggactg tttatcttca gtcagcagga
    12661 taatgaaccg gtggcgctgg tgactaaatt tgcaggcgct gagatgcgcc agacgctgac
    12721 gctgcacaaa ggggcgaatt atatttcgct gacgcagaac attcagcaat ctggcttgtt
    12781 aagtgcagaa ctgcaacaaa atgggcaagt gcaggacagc attagcacaa aactgtcttt
    12841 tgtggataac agctggcccg ttgaacagca gaaaaatgtc atgctcggtg gtggcgataa
    12901 cgcgctgatg ttgcccgagc aggcgagcaa tatccggcta caaagtagtg aaacgccgca
    12961 ggagattttc cgcaacaatc ttgatgcgtt agtcgatgaa ccgtggggtg gggtgatcaa
    13021 caccggtagc cgactgatcc cgctcagtct cgcctggcgt tcgcttgccg atcatcaaag
    13081 tgccgccgct aacgacattc gtcagatgat tcaggataac cgtctgcggc tgatgcaact
    13141 ggcggggccc ggagcgcgct ttacctggtg gggtgaagat ggcaatggtg acgccttcct
    13201 tacggcatgg gcatggtacg ccgactggca ggccagccag gcgctcggcg taacgcaaca
    13261 accggaatac tggcagcata tgctcgacag ttatgccgag caggcagata acatgccgtt
    13321 attgcatcgg gcgctggtgc tggcgtgggc acaggagatg aatctgccgt gcaaaacgtt
    13381 gttgaaaggg ttggatgaag ctatcgcccg gcgcggaact aaaactgaag atttctctga
    13441 ggaagacccc cgcgatatca atgacagcct gatcctcgat acaccggaat ctccactggc
    13501 agatgcggtg gcaaacgtct taaccatgac tttgctgaaa aaagcgcagt tgaagtccac
    13561 ggtgatgcca caggttcagc aatatgcgtg ggataaagcg gcaaacagca atcagccgct
    13621 ggcgcacacg gttgtgctgc tcaatagcgg gggcgacgct acccaggcgg ctgctatttt
    13681 aagtggtttg accgctgagc aatctactat tgagcgcgcg ctggccatga actggctggc
    13741 gaaatatatg gcgacaatgc ctccagttgt tttgcctgcg cctgcgggcg catgggccaa
    13801 acataagtta actggagagg gtgaatactg gcgttgggtt ggtcagggcg tgccggacat
    13861 tctctctttt ggtgacgaat tatcgccgca aaatgtgcag gtccgctggc gtgaaccggc
    13921 aaaaacggct caacgaagta acattccggt gaccgttgaa cgccagttgt atcggcttat
    13981 ccccggtgaa gaagagatga gctttactct gcagccggtg accagcaatg agattgacag
    14041 cgatgcgctg tatctcgatg aaattacgct taccagcgag caggatgcag ttctgcgcta
    14101 tggtcaggtg gaagtaccgc tcccgccggg agccgacgtt gagcgcacaa catggggcat
    14161 ttcagtcaat aaacccaacg ccgcgaaaca gcaggggcaa ttgctggaaa aagcacgtaa
    14221 tgaaatgggc gaactggcct atatggtgcc ggtgaaagaa ctgacgggaa cggtcacttt
    14281 ccgccatttg ctgcgcttct cgcaaaaagg gcaattcgtt ctgcctcctg ctcgttatgt
    14341 gcgttcctat gcacctgcac agcaaagtgt tgcggcaggg agcgaatgga ccgggatgca
    14401 ggtgaaataa gtgaactggc gcagaatcgt ctggctattg gcgttggtta ctcagccaac
    14461 gcttgcggag gagacgccgt tacaactggc gctgcgcggt gcgcagcacg atcaactgta
    14521 tcagctgtcg tcatcaggcg tgaccaaagt ctcagcatta cctgactcgc tcaccacgcc
    14581 gttagggagt ttgtggaaac tgtatgttta cgcctggctg gaggataccc atcaaccgga
    14641 gcagccctac cagtgtcgcg gaaactcccc ggaagaggtt tattgttgtc aggctgggga
    14701 gagcataacg cgcgataccg cgctggtacg ttcctgtggg ctctattttg cgccgcagcg
    14761 gttgcatatt ggcgcagatg tgtgggggca atactggcaa cagcgtcagg cacctgcgtg
    14821 gttagcgtct cttactacgc tgaagccaga aacctcggta acggttaaat cgctgcttga
    14881 ttcattagcg acgttacctg cgcaaaacaa agctcaggaa gtcttactgg atgtggtgct
    14941 ggacgaggcg aaaatcggtg tcgcatccat gctcggtagt cgggttcggg tgaaaacctg
    15001 gagctggttt gccgatgata aacaggaaat ccgtcagggt ggttttgccg gctggttaac
    15061 cgatggtaca ccgctgtggg ttactggcag tggcaccagc aaaacggtac tgacccgtta
    15121 tgcgacggtg cttaatcggg ttttgccagt gccgacacaa gttgccagtg ggcaatgtgt
    15181 agaggtggag ctatttgccc gctatccgct gaagaaaatc acagcggaaa agagtacgac
    15241 ggcagtaaaa cccggcgtac ttaatgggcg ctatcgtgtg acatttgcca atggtaacca
    15301 cattacgttt gtcagccacg gtgagacgac cttactgagc gaaaagggca aactgaaatt
    15361 gcagtcgcat ctcgatcgtg aagagtacgt tgccagagtt ctggatcgcg aggcgaaaag
    15421 tacgccccct gaggcagcga aagccatgac cgtggcgatt cgcacttttt tacagcaaaa
    15481 cgccaatcgg gaaggcgatt gcctgaccat ccctgacagc agcgccacgc agcgtgtttc
    15541 ggcttcgcca gctaccacgg gggcgcgaac gatgaccgcc tggacgcagg atctcatcta
    15601 tgcgggcgat ccggttcatt atcacggcag tcgcgctacc gaagggacgc tttcctggcg
    15661 gcaagccatg gcccaggcgg ggcagggaga gcgttacgac cagatactcg cgtttgctta
    15721 tcccgacaac agccttagcc gctggggagc gccacgctca acctgtcagt tattacccaa
    15781 agcgaaagcc tggctggcga aaaaaatgcc gcagtggcgg cgtatattac aaggcgagac
    15841 ggggtacaac gaaccagacg tgtttgcggt ctgtcgttta gtctcaggtt tcccctatac
    15901 cgatcggcag cagaaacggc tgtttattcg taacttcttt acgcttcagg atcggctgga
    15961 tttaacccat gagtatctgc acctggcctt tgatggttat cccaccgggc ttgatgagaa
    16021 ctatatcgaa acgctgaccc gacaattatt aatggactaa tgctatgcga aagatttttc
    16081 ttccgttgtt actggtggcg ctttcgcctg tcgctcacag tgaaggtgtg caggaagtcg
    16141 agattgacgc accgctctct ggctggcatc cggtagaggg cgaagatgcc agtttttcac
    16201 aatcgatcaa ttacccggcg tcgtccgtca acatggcaga tgatcaaaat atctcagcgc
    16261 agatccgcgg caaaataaag aattatgctg cggcgggtaa agttcagcag ggccggctgg
    16321 tggtcaacgg tgccagtatg ccgcagagaa ttgaatccga tggttcattt gcacgccctt
    16381 atattttcac tgaaggcagc aacagcgtgc aggtcatcag cccggatggg caaagccgac
    16441 aaaaaatgca gttttactca acgccgggcg ctggagtgat tcgcgcacgt ttacggctgg
    16501 ttctctcgtg ggatacggac aataccgacc tcgatcttca cgttgtcacg cccgatggcg
    16561 aacacgcctg gtacggtaac accgtgctga aaaacagtgg tgcactggat atggatgtca
    16621 cgacggggta cggacccgag attttcgcca tgccagcgcc agttcacgga cgctatcagg
    16681 tgtatatcaa ctactatggc ggacgcagcg aaacggaatt aaccaccgcc caactgacgc
    16741 tgatcaccga tgaaggatcg gtcaatgaga aacaggaaac atttattgta ccgatgcgta
    16801 atgctggcga gctgacgctg gtgaaaagtt ttgactggtg atccatccct tcacacaacc
    16861 tggctctttc ttttttcaga taagactgct gggcgatctg ccgacgccag gatataacca
    16921 ttaacaagcg tcggcagatt ggtcatttct tatttttcgt actgagaact gtccatttac
    16981 tccggctttc ctggttaagg caatggtttc taagcgatac gcattattgc aatggtgaca
    17041 gaacattgcg gtaacacgct tttaccgcta ccttaaccac actccatcgg tcacctgagg
    17101 cggagcttcg cccctttgaa ataccttgct tcttttcgta caaccctgaa agcctcgcgc
    17161 tacatgttca gagcattggc gttagtgctc tggctgttga ttgctttttc atccgttttt
    17221 tacatcgtta atgcgttaca tcagcgagaa tcggaaattc gtcaggaatt taatctgagt
    17281 tccgatcagg ctcagcgctt tattcaacgc acctctgatg tgatgaaaga gctgaagtac
    17341 atcgccgaaa atcgcttatc ggcagaaaac ggtgtgcttt ccccgcgtgg acgagaaacg
    17401 caggcggatg tgcctgcgtt tgaaccgctg tttgccgact ccgattgttc cgcaatgagt
    17461 aacacctggc gaggttctct ggagtcattg gcgtggttta tgcgctactg gcgcgataat
    17521 ttttctgcgg cttacgatct caaccgggta tttttaatcg gcagcgataa cctctgcatg
    17581 gccaatttcg gtctgcgtga tatgccagtg gaacgcgata ccgcgttgaa agcgttgcat
    17641 gaacgcatca ataaatatcg aaatgcacca caagatgata gcggcagtaa cctctactgg
    17701 atcagcgaag gtccgcgccc tggcgtcggg tatttttacg cgttgacgcc agtttatctg
    17761 gcgaaccggt tgcaggcgct tttgggtgtc gagcagacca tccggatgga gaactttttc
    17821 ttaccgggta cgttgccgat gggggttacc attcttgatg aaaatggtca taccctgatt
    17881 tcgcttaccg gaccagaaag caaaattaag ggcgatcctc gctggatgca ggaacgctcc
    17941 tggtttggct atacggaagg tttccgggag ctggtgctga agaaaaatct gcctccctca
    18001 tcgctaagca tcgtttattc ggtgccggtt gataaggtgc tggaacgcat tcgcatgttg
    18061 atccttaacg caattttgct gaatgtgctt gccggagctg cattgtttac tctcgcgcgg
    18121 atgtacgagc ggcgtatttt cattccggcg gaaagcgacg ccctgagact ggaagaacat
    18181 gagcagttta accgcaagat tgtcgcctcc gcgccagtgg gtatctgtat tttgcgtacc
    18241 gctgatggcg tcaatatttt aagtaacgaa ctggcgcata cctatctcaa tatgcttacg
    18301 catgaggacc gccaacggct gacacaaatt atctgtgggc agcaggtcaa ttttgttgat
    18361 gtcctgacca gcaacaatac caatctgcaa atcagcttcg tccattcgcg ctatcgtaat
    18421 gaaaacgtgg ccatttgtgt gttggtggat gtttcttcgc gcgtgaagat ggaagagtcg
    18481 ttgcaggaga tggcacaagc agcggaacag gcgagccagt caaaatcgat gttccttgcc
    18541 accgtcagtc atgagctgcg aacgccgctg tatggcatta tcggtaacct ggatctgttg
    18601 caaaccaaag agttaccgaa aggcgtcgat cgactggtga cggcaatgaa caactcttcc
    18661 agcctgttgt tgaaaattat cagcgatatt ctcgatttct cgaagattga atcggaacag
    18721 ttgaagatcg aaccgcgtga gttttcaccg cgtgaagtga tgaaccacat caccgccaac
    18781 tatttaccgc tggtggtacg caagcagtta ggcttgtact gctttattga accggatgtg
    18841 ccagtggcct taaatggcga cccgatgcgt ttacagcagg tcatctccaa cctgttgagt
    18901 aacgccataa aattcaccga taccggctgt atagttttgc atgtccgcgc tgatggcgat
    18961 tatctctcta tccgtgttcg cgataccggc gtggggattc cggcgaaaga agtggtgcgc
    19021 ttgtttgatc ccttcttcca ggtcggaacg ggcgtacagc gaaacttcca ggggaccggt
    19081 ctgggtctgg cgatttgtga aaaactgatc agcatgatgg acggcgatat ctcggtagat
    19141 tcagaaccgg gaatgggcag tcagtttacc gtgcgtattc cgttgtatgg cgctcagtac
    19201 ccgcagaaaa aaggcgtgga agggttgagt ggtaaacgct gctggctggc ggtccgcaat
    19261 gcgtcgctct gtcagttcct ggaatccagt ttgcagcgca gcggcatcgt cgtaacaaca
    19321 tacgaagggc aggaaccgac tcccgaagat gtgttaatca ctgatgaggt agtgagtaaa
    19381 aaatggcagg gcagagcggt agtgaccttc tgtcgtcgac atattggtat tccgctggag
    19441 aaagcgccag gggagtgggt acacagtgtg gctgctccgc atgagctacc ggcattgttg
    19501 gcgcgtattt atttaatcga gatggagagc gacgatcctg ctaacgctct gccgtcgacg
    19561 gacaaagcgg tcagcgataa tgacgatatg atgattctgg tcgtggatga tcatccgatt
    19621 aaccgacgtc tgctggcaga tcagttggga tcgttgggct atcaatgtaa aaccgcgaat
    19681 gatggcgtcg atgcgcttaa tgtacttagc aagaatcata ttgatatcgt gcttagcgac
    19741 gtcaacatgc caaatatgga tggttaccgc ttgacgcaac gcattcgtca gttgggactg
    19801 acgttaccgg taatcggagt aactgctaat gcgttggctg aagagaagca gcggtgtctg
    19861 gagtccggta tggacagctg cctgtcgaag ccggtaacgc tggatgtgat aaaacagacg
    19921 ctgacggtat atgccgagag ggtcaggaaa tcgcgggaat cgtaggacgg ataaggtgtt
    19981 tatgccgcat ccggcattca gtgcaaatgc cagatgcgac gctgacgcgt cttatctggc
    20041 ctactttaat gtaatctgtt gaaataatgg gaatcgtagg ccggataagg cgtttacgcc
    20101 gcatccggca atcggtgcta acgcgtctta tctggcctac agatgattag tctttatctg
    20161 ccggacttaa ggtcacagaa gagagataat tcagcagggc gatatcgttc tcgacaccca
    20221 gcttcatcat cgcagatttc ttctggctac tgatggtttt aatactgcgg ttcagctttt
    20281 tagcgatctc ggtcaccagg aagccttccg caaacaggcg cagaacttca ctctcttttg
    20341 gcgagagacg cttgtcaccg taaccaccag cactgatttt ttccaacagg cgagaaacgc
    20401 tttccggggt aaatttcttc cctttctgca gcgcggcgag agctttcggc agatcggtcg
    20461 gtgcaccttg tttcagcacg atcccttcga tatccagatc caataccgca ctaagaatcg
    20521 ccgggttgtt gttcatggtc agaacaataa tcgacaggct tgggaaatgg cgcttgatgt
    20581 acttaattaa ggtaatgcca tcgccgtact tatcgccagg catggagaga tcggtaatca
    20641 acacatgcgc atccagtttc ggcaggttgt tgatcagtgc tgtagagtct tcaaattcgc
    20701 cgacaacatt cacccactca atttgctcaa gtgatttgcg aataccgaac aagactatcg
    20761 gatggtcatc ggcaataatt acgttcatat tgttcatgta ataggctacc ttgctacagc
    20821 aagctcttga cataactgtc aatgtcgctg atgtattttt ctattcctgg aacatccttc
    20881 tcacgaatca gatgttccag cgtttcacat aactgcttgc cgggtaccag atttagcatg
    20941 gcaaatacgc ctttaagacg atgagccgtt tgggctaacg cagcaaagtc actggttgct
    21001 gcttcagtat acagcctctt aacatcatcc ggtactgtgt ctacaaagag cgcataatag
    21061 ccgctggcat ggagttgcgc attttcatct ccgcccagag gcgattctgt cacctcttcc
    21121 tgcgccagtt gcacttcaat taattgtaag accgcttcct gcatagcgtt gctcatattg
    21181 aagttgacgc acaattgacc aggcccaatt tcccgtacgc cagactcatc atcgcttaaa
    21241 agcaagccag aggcagtaag attagacgga ttatccgtta aaaagatatc ataatcttga
    21301 ctaattaatc tttcatcggg tgtgatacag gttgcacccc aattttctaa ctggcgagtg
    21361 acaatattcc gaatttctgc cgaagtaaca tccaccatta cgcagacatc atccagtaaa
    21421 cgctcttctt cctcttcaac ttccgggtca gctgcgagca ttttgatatg cacagagtag
    21481 cgtgtaccaa gcccatcccg cgttttgatg tttaaatgac cgcccagttt acgtgccagt
    21541 tgatcgctca gccagaatgc cagcgggtcc gccttgccat agcgatcgtt ttgggtctgg
    21601 ttgataaacg ggaagtgcaa attatccatt tcatgaatgc ttacgccttc tccggtgtcc
    21661 agaatgcgga acgtcaggcg gtcttcggag gactcatcct gatcaacctc aagggtgatt
    21721 tttcccaatt gcgttgaggt cacggcatat tgcatcagta gcagcaaaat acgtcgtaag
    21781 gcatcgcgat cgccacggcg catatcgtgt gctttcagat gattgttaat cagcagttgc
    21841 agacctttac gcttgatggc aggcaacact gaaggcacaa cttcatcaat taaatcctgc
    21901 acggagaaca gcaccgtctc acttttccag ctatcgtccg caagcatgtt cgctaactgt
    21961 atttcatcga ccagccgcac cagcacatct gcctgattcg ccagttgttt gctttccggg
    22021 gcgttgagtt tagccgcgct ctccgccagg gactgtgcag gttctttcag cgcatcgcca
    22081 atgtttttca taaaggtcat ccgcccctgc tggtttttct catacagacg ctgcgcctgc
    22141 ttgagtttct tgtttaccag cacttcgcga tcctgatcgc gaataatgaa aatttgtgtg
    22201 cgcggcgcga cctggctgcg gaacatgcgg atctcataca gctcgttatt gatcgtcgcc
    22261 tgaataatcc cctgatgctg ttccgccatg gtggtgatgt tttgcagatt caaatgcggc
    22321 agcaaatgat cggcaatttt gttacttatg acagtgcggt tcgattcctg atcgtgaacc
    22381 agcaggccga gcggcagcag tgagactatc tcttcattga ttgcccgtaa aatgcgcaat
    22441 tcgttattga ccgccgtgtt gggtagactt tctgtactgc gactggagaa atggcggaat
    22501 gtggtatagc caaataacgc cagcgccagc aaaccgatgt tcagcagcag tggcagcaga
    22561 atgttttgca acgtatccag caataaggtg ccataaggaa cctgccagac cagtcgcata
    22621 tcggtagagt tgagtgccga ggagatttca atcttcgtac tgttaaagtg gatactgacg
    22681 ctatccgtcc cttctttctc attatcattg tctcccgttg ccgtcgcgtc tggctcaagg
    22741 cggaaactgt ccagcggcat acccggtggg atcaaatcat taatcggcag atcaaaagcc
    22801 acgaccgttg ccagatgtcc cggctggttg aacgtggtac gcaaggtaaa gtaatgaccg
    22861 ttctgccagg ccaggcggcg catgttagaa aaactttcgc gttcatcgag ggcgttggcc
    22921 tgttgcaaca tctctgcacg acgtgaatca acaatgtcac tgacggtcga ttctttaaat
    22981 ccggaggtga gatctttgag gggcagggtt gagatcagta ccagactgtt atcctgacca
    23041 ttcaggtaat acattgacca cggtacattt tctgcgcccc acaatgtgtc cagataggtg
    23101 gacatccgtt gagtcatctc aagcgttgag ttgtcgtgag agccaaagat taacgcttcc
    23161 gttttgcggc gcggtttttc cagatagtag acatcctgtt tcaggcgcgt ctcttgtaaa
    23221 ccttcgccgg aggaggggga ggtcgtcgcg gcaatgttgt cgtagatctg ccaggtcacg
    23281 taacgccagg tatcgacgcg cttttggata gcgtgggtaa tgtcgacaat ctggtaactt
    23341 ttatctttca gccaggcgtt aacggcgctt tgtaccatta cacccatcgt caccagtaac
    23401 acaatgatca acagtaaaaa gaagcgggta atgctccccg gtaggagtga aaagcgggtc
    23461 gtggccgttg tctctttctg acgcattcgt gtttatgacc tgttaaaact tcgcgaagtg
    23521 atagggcagt ataaagggta cagtgtgaaa ttccagactc ttacctgatt gatgattctc
    23581 tattctcctg tgatatttat catcctgtat ggggggaaat agatgtacaa gcgccatttt
    23641 gctgtacaaa taacctacga aaagcccaac gaaattaatt gcaaataaac gaaaattgct
    23701 ggaaattatg ctgatgttaa ttatttgtga aatagttaac aagcgttata gtttttctct
    23761 ggtagcacag aataatgaaa agtgtgtaaa gaagggtaaa aaaaaccgga tgcgaggcat
    23821 ccggttgaaa taggggtaaa cagacattca gaaatgaatg acggtaataa ataaagttaa
    23881 tgatgatagc gggagttatt ctag
//
LOCUS       DAPGOF010000069        23776 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.069, whole genome shotgun sequence.
ACCESSION   DAPGOF010000069 DAPGOF010000000
VERSION     DAPGOF010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.069"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..3878)
                     /gene="entF"
                     /locus_tag="RHV13_002345"
     CDS             complement(<1..3878)
                     /gene="entF"
                     /locus_tag="RHV13_002345"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HDV1241872.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLPQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQITNIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRRQLPPPASLSPAPLPVR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPKLATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKKLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYQFSYREMREQVVALANLLRERGVVPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDHLPRF
                     ADVPDLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTSV
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLSQLPLSANGKLDRKALPLPELKAQAPGRAP
                     KAGSETIIAAAFSSLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYI
                     DPQWSIIGIQSPRPNGPMHTAANLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGGQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFEKIGPIIRATLN"
     gene            complement(3875..4093)
                     /locus_tag="RHV13_002346"
     CDS             complement(3875..4093)
                     /locus_tag="RHV13_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family protein"
                     /protein_id="HDV1241873.1"
                     /translation="MAFSNPFDDPQGVFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANYTQLQEAQ"
     gene            complement(4096..5298)
                     /gene="fes"
                     /locus_tag="RHV13_002347"
     CDS             complement(4096..5298)
                     /gene="fes"
                     /locus_tag="RHV13_002347"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HDV1241874.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5541..7781
                     /gene="fepA"
                     /locus_tag="RHV13_002348"
     CDS             5541..7781
                     /gene="fepA"
                     /locus_tag="RHV13_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HDV1241875.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            7956..8576
                     /gene="entD"
                     /locus_tag="RHV13_002349"
     CDS             7956..8576
                     /gene="entD"
                     /locus_tag="RHV13_002349"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HDV1241876.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(8698..8850)
                     /gene="hokE"
                     /locus_tag="RHV13_002350"
     CDS             complement(8698..8850)
                     /gene="hokE"
                     /locus_tag="RHV13_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HDV1241877.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGESLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(9201..9353)
                     /gene="hokE"
                     /locus_tag="RHV13_002351"
     CDS             complement(9201..9353)
                     /gene="hokE"
                     /locus_tag="RHV13_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HDV1241878.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            complement(9540..9731)
                     /locus_tag="RHV13_002352"
     CDS             complement(9540..9731)
                     /locus_tag="RHV13_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1241879.1"
                     /translation="MFLFFGDGELMVPEAIWLLLQCDKLWQSWLVAKNFDDWGAQNKT
                     GSASVDQMRSEKSETRLTV"
     gene            9806..10924
                     /locus_tag="RHV13_002353"
     CDS             9806..10924
                     /locus_tag="RHV13_002353"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HDV1241880.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNE
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRSLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERTFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRALTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            10990..11238
                     /locus_tag="RHV13_002354"
     CDS             10990..11238
                     /locus_tag="RHV13_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HDV1241881.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTTFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            11303..11671
                     /locus_tag="RHV13_002355"
     CDS             11303..11671
                     /locus_tag="RHV13_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDV1241882.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            11765..12418
                     /gene="nfsB"
                     /locus_tag="RHV13_002356"
     CDS             11765..12418
                     /gene="nfsB"
                     /locus_tag="RHV13_002356"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HDV1241883.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            12526..13773
                     /gene="ybdG"
                     /locus_tag="RHV13_002357"
     CDS             12526..13773
                     /gene="ybdG"
                     /locus_tag="RHV13_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HDV1241884.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(13841..15217)
                     /gene="pheP"
                     /locus_tag="RHV13_002358"
     CDS             complement(13841..15217)
                     /gene="pheP"
                     /locus_tag="RHV13_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HDV1241885.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(15319..18462)
                     /gene="cusA"
                     /locus_tag="RHV13_002359"
     CDS             complement(15319..18462)
                     /gene="cusA"
                     /locus_tag="RHV13_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HDV1241886.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLGEVKNALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQVGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMQMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(18474..19697)
                     /gene="cusB"
                     /locus_tag="RHV13_002360"
     CDS             complement(18474..19697)
                     /gene="cusB"
                     /locus_tag="RHV13_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HDV1241887.1"
                     /translation="MKKIALIIGSMIAGGIISAVGFTWFDRAEPPAEKTSTAERKVLF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLSATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(19713..20045)
                     /gene="cusF"
                     /locus_tag="RHV13_002361"
     CDS             complement(19713..20045)
                     /gene="cusF"
                     /locus_tag="RHV13_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HDV1241888.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISASGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(20069..21451)
                     /gene="cusC"
                     /locus_tag="RHV13_002362"
     CDS             complement(20069..21451)
                     /gene="cusC"
                     /locus_tag="RHV13_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HDV1241889.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNADWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLKITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            21608..22291
                     /gene="cusR"
                     /locus_tag="RHV13_002363"
     CDS             21608..22291
                     /gene="cusR"
                     /locus_tag="RHV13_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HDV1241890.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            22281..23729
                     /gene="cusS"
                     /locus_tag="RHV13_002364"
     CDS             22281..23729
                     /gene="cusS"
                     /locus_tag="RHV13_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HDV1241891.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQGGEVYLLSGPTMMMPGHGHGHMEHSNWRMISLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASIISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELQFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PGEAIVVRCQTVEHQVQVIVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIVLPERG"
BASE COUNT         5718 a         6499 c         6049 g         5510 t
ORIGIN      
        1 ctgtttagcg ttgcgcgaat aatcggccca attttttcaa acgcccctgg agagataata
       61 tccacatgcg cacaatcctg acgatagata tccagctccg caatccacgg cgaccaggcg
      121 cgttcaggac tcataccttc ctgaagcgtg cgttcagcaa caaacaacgt cgctttaccg
      181 tcaaacggta cgctatgagc agtcgtcagc aggcgcacag catcagcgta gttgccttca
      241 atggtggtaa acaactccgt tgaagtactt ccctgctgtg ctgccaggaa ggcctcgcgt
      301 tcgcggttaa tctccgccag cacttccggg tccagaccat tagcttcttt ttcctgccag
      361 ttttgcgttt ctggcggcca ggtatccagc aagccaagaa atgccacctg tccgccacgg
      421 gcacgcagcc gcgccgcaat accctgcgcc agcgtaccgc caagtgaata acccagcagg
      481 taataagggc cgtgcggttg ttgttcaagt aaagttgcca gatgcgcttc gcagacttca
      541 tccagatttg ccgcggtatg catggggcca ttggggcgcg gcgactgaat gccgataatc
      601 gaccattgtg gatcgatata acgcgagagc acgctaaact gccaggcaaa accggatgcc
      661 ggatggaaac aaaacagcgt cgggccatta ccttcacgca acggcagaat ggtttcgaat
      721 cccatacgcc gggagctgtc ctcttcacca tcaataatcg ttgccagttt ggcgacagtt
      781 gacgcgacca tcacctgccc cggcgtcacc tgacgggcaa actgccgact taactgcgct
      841 gccagtttca ttgccagtag cgaatgaccg cccagcgcaa agaaatcagc atcggcatcc
      901 tgcacgtcac accccagcaa cgacgagaat gccgcggcga taatcgtttc actgcccgct
      961 ttcggcgcac gccctggcgc ttgtgccttc agttcaggca acggtaaggc tttgcgatcc
     1021 agcttgccgt tggcgctaag tggtaactgt gaaagttgca gcagaacgac cggcaccata
     1081 tgcggcggca atgtttcacg aagctgcgcc tgcaatgcgc tggtatccaa cggcaggccc
     1141 gattgcgaca ccagatagcc caccaactga cgcgcatcac caccagtggc tgccgcctgg
     1201 ttaatcacac aggcgtgggt aacggcttgt tcgacatccg gcagcgcctg catcacgcga
     1261 tcgatttcgc ccagttcgat acgctgcccg cgaattttta gctgatcgtc actgcgcccg
     1321 aggtactcca ccgcgccgtt atccagccag cgggcaacgt ctccggtacg gtacatccgt
     1381 tcgccaggag caaaaggatc ggcaataaaa cggctggcgg tcagatcggg tcgtccaaga
     1441 tacccctgcg ccagttgaat gccggtgaga tagagatcac ccgccacacc cggcggcacc
     1501 ggatgcatca tcgcatcaag aatacgcaga cccgtattcc ataccggata accaatcggc
     1561 acactgctgc cgcgcacctg tgccagttcc tcgccaaaag ccggatacca gctgacatct
     1621 accgccgctt ccgtcgggcc atatagatta tgcaacggca cgctggttaa ctgttgccat
     1681 tcgcggcata aatcagccgg taaggcctca ccactacaga aaacctgttt caacgtcgcg
     1741 caactctggc gagcggtttg cggcgtcagc gaggcaacaa atgccgccag catcgacggc
     1801 acaaagtgcg tggtcgttac gccatattcg gcaaagaatt gctgcatagc gagcgggtcg
     1861 cggtgcgctt ccggttcagc catcactagt ttagccccgg caataaacgg ccagaaaaac
     1921 tcccacaccg agacatcaaa actgcacggc gttttttggg caacgacatc ttcacctgta
     1981 agtggataat gattttgcat ccacaatagg cggttaacga tagccgtctg cccgaccatt
     2041 acccctttcg gcctgccggt ggagccagag gtaaagatga tataagccgt gtgatgcggt
     2101 tgtgaaagtt gcagcggcgc actgccctgc ggtgtaagcg gggcgttata gcaaaggttg
     2161 gttaaatctg gaacatcggc aaagcgcggc agatgatcgt cggtggtgat taacagcgac
     2221 ggacgcgcat cttccagcat cattttcagg cgatcgtccg gataaccggt atccagcggt
     2281 agccaggccg cgccagcttc aactatcgca tgtagcgcca gggtcaaaaa gaccgagcgc
     2341 ggtaatgcca ccgccacgct gtcccctggt acaacgccgc gctcacgcag cagattcgcc
     2401 agcgccacca cctgctcgcg catttcccga tagctgaact ggtaacgcgc atctgccagc
     2461 gccggagcat ccggtgtttt tgccgcttgt tctgccacca gcgcgctaag cgtggtttct
     2521 ggaatctcaa cctgagtggc gttgatctgc gccagctgcg catactcacc tggcagcata
     2581 atatcgacat cgccgcacaa cagcgccgga tccgcggcga attgggcgat cagctttttc
     2641 aggcgttcag catgctggat taacgttggc tcatcgtaac gctgtttatt ggcgaggatc
     2701 tcaatactca aatcaccgtg ttcatccggg aacagggcca gttcaaggtc attaaccgga
     2761 ccggttgcca gggtatgggt ttgcgcctga acaccaggaa tatccagttg gtaatcaaat
     2821 accttgatat tgagtaccgg accaaacagc ggttcatcac ctgccgctcg cccgctgtca
     2881 cggacaattt gttcggcatc gtaacgttga tggcgacgca tttttttcag ttgtgctgcc
     2941 agtcgggttg ccagtttcgg cagcgtttct tgcgccgcaa tgtgaatacc caacggcaaa
     3001 acgttgagca cgggtccggt agccgtcagc gccgccgagc ccagtcgacg cataaagata
     3061 aatccggcgg cgtagtccat acgattgcac aatcgcccca gccacaaggc tgccagcgca
     3121 agggctaaat cggtacgctg cacacctgaa agttgcgtag ccagctggcg gaattccccg
     3181 tcggtaaatt ccagtttcag gcgcagaata tctgccgagg cgctgcgcac cggtaaaggt
     3241 gccggagaaa gtgacgcggg cggtggcagt tggcgacgct gttccaccca gaatgccgca
     3301 tcacgctgcc aggcttcgct ttcgcggtat tgctggtact cttccactac atcagcgaaa
     3361 ggcgtaaacg gcgaagcagg cgttggttcg ccacgcagca atgcgcagta aatgttggtg
     3421 atctggcggg taatggccgg aaaactgaag ccatcgacca gtaaatggtg ataacgctga
     3481 taccagtacc agcggttatc tgccacctgt atcagctggt gaaagaccag tggtttaccg
     3541 ctatcgacgc gcaaatcttg ttgcaaatcc gcctgcatta atgcctgcgc agtaccgtgc
     3601 ggatcaatat tggttcgcag gtcgataatt tctggcagtt cgaatatcag cgcatcatcg
     3661 acccattgcc agacttcgcc gttatcttcc gtaaaacgca tccgcaacgt atctgcttgc
     3721 ggtagtccgg caaccaccgc gcgggccagt aatggcgcat caacctctcc ggttaactca
     3781 acgtaatgcg ccacgctcca ggcggagggt aaatccgaca gtttttctgc catccagatg
     3841 ccgggctgtg cggcgaccaa aggtaaatgc tggctcattg tgcctcctgc aactgggtgt
     3901 aattcgccgg tgtcagagta cgccagtggg cttcaagcca ctgctggcag gactcttgtg
     3961 actgcggctg acacacaatg tcccagcctg ccggtaaggc gcattgttgc ggccacagac
     4021 tgaattgccc ctgcgcattg cgcaaaatgt aaaacactcc ctgtggatca tcgaagggat
     4081 tactgaatgc catattcaac tcctgtcatg gaaaagtggt tgccagagat cgattaaccc
     4141 ctgcatcaag ccaccgcgcc aacaaagcgc atcatgtccg ccgtcaacct gacgccagaa
     4201 aatggattct tttatagggt gcaattgcgc atacagcgcc tgattggccc gcatgatcat
     4261 cggctcgcga atacccgctt ccagcacaat gcgcagacct tcggcgctaa cttcaccagc
     4321 ttttagcttt tcaagtaaca cgccctcttg ctgcccgccc cgatgcggcc accagtacga
     4381 tcctgactgg cttaatacac agccaaagcg ttcaggccag tgcagtccgg catacagcgc
     4441 ggacagccca ccaaaactct gcccggcgac cacggtgcga tcggcgcgat cgctaaaagg
     4501 ggcaatagct ttcaccaggg gtaataactc ttgctgtact gccagccaga aatccgcatt
     4561 acacggcagt tcgtgggcgc ggtgcgtggt gtcgatagcg tcgatcaaca catacacggc
     4621 gggaggaagt tgctgacgat gggtcagcga agtcagcaca ggccagacgg gcatactttg
     4681 cgcccaaaat tcgccatcga gcaaaactgc cagcgggcgt tcttctgctg ttacatcgcc
     4741 agtggtaaaa atccatacac gacgtgaatt tttcaaccgt tcacttttcc agataatttc
     4801 tttggctggt atttctggcg cttgcggaca atcccatccc ggttgcagag gcgcttgcgg
     4861 catttcgagt gcagaaacag cgtgccctcg cccgcctttc cagctttgtg gattcagcgg
     4921 atcggctatt gcctggggta atagttttcg ccagccttca cgtaattcga ggcgatcggg
     4981 ggatggtgca gaaaaaatgt catcgcgttc ggtgggaata aagcagtagc tgccgcgcca
     5041 gttggcattg agttgtgtcg tccactgcca gacatcagta cctgcaattc gctgcatcga
     5101 ctggggctgg ctgttctgat gatgatcggt cacaccagtg atgtagaccc atacgcgctt
     5161 tatcgtcgag tattcttcag aaccttgggg atcacgccac cagaaagtga cctcaaacat
     5221 ttcgtcattc agacgctgcc attccgggcc atgtttcgac tgccaccagc tctcacttcc
     5281 tacttttaac gccgtcacac cataacccca tgtttactgt gcaatttttc attgattgca
     5341 gaaatatatt gataatatta ttgataacta tttgcatttg caatagcgta atggcgcgcc
     5401 gtgggaagcg cggacattaa ttaaccaact gcactgcgtg tctttcagga tcaaaggttt
     5461 tcgcggtagc gggatgcgtc gtgttgatga cgaccatgcc cgacagttgc aattcgtggc
     5521 aaaaatgcag gaataaaaca atgaacaaga agattcattc cctggccttg ttggtcaatc
     5581 tggggattta tggggtagcg caggcacaag agccgaccga tactcctgtt tcacatgacg
     5641 atactattgt cgttaccgcc gccgagcaga acttacaggc gcctggcgtt tcgaccatca
     5701 ccgcagatga aatccgcaaa aaccccgttg cccgcgatgt gtcggagatc atccgtacca
     5761 tgccaggcgt taacctgacc ggtaactcca ccagtggtca gcgtgggaat aaccgacaga
     5821 ttgatattcg cggtatgggt ccggaaaaca cgctgatttt gattgacggc aagccggtaa
     5881 gcagccgtaa ctcggtgcgt cagggctggc gtggcgagcg cgatacccgt ggtgatactt
     5941 cctgggtgcc acctgaaatg attgaacgta ttgaagttct gcgtggtccg gcagctgcgc
     6001 gttatggcaa cggcgcggcg ggcggcgtgg ttaacatcat taccaaaaaa ggcagcggcg
     6061 agtggcacgg ctcctgggac gcatatttca atgcgccaga acataaagag gaaggtgcca
     6121 ccaaacgcac taacttcagc ctgaccggcc cgctgggtga tgaattcagc ttccgtttat
     6181 atggcaatct cgacaaaacc caggctgacg cgtgggatat caaccagggc catcagtccg
     6241 cgcgtgccgg aacgtatgcc acgacgttac cagccgggcg cgaaggggtg atcaacaaag
     6301 atattaatgg cgtggtgcgc tgggacttcg cgcctctgca atcactcgaa ctggaagcgg
     6361 gttacagccg ccagggtaac ctgtatgcgg gcgataccca gaacaccaac tctgacgctt
     6421 acactcgctc gaaatatggc gatgaaacca accgcctgta tcgccagaac tactcgctga
     6481 cctggaacgg tggctgggat aacggcgtga ccaccagcaa ctgggtgcag tacgaacaca
     6541 cccgtaactc gcgtattccg gaaggtctgg cgggcggtac cgaagggaaa tttaacgaaa
     6601 aagcgacaca ggatttcgtc gatatcgatc ttgatgacgt gatgctgcac agcgaagtta
     6661 acctgccgat tgatttcctc gttaaccaga cgctgacgct gggtacggag tggaatcagc
     6721 agcggatgaa ggacttaagt tccaacaccc aggcactgac cggaacgaat accggtggcg
     6781 ctattgatgg cgtgagtgcc accgaccgta gcccgtattc aaaagcagaa attttctcgc
     6841 tgtttgccga aaacaacatg gagctgactg acagcaccat cgtaacgccg gggctgcgtt
     6901 tcgatcatca cagtattgtc ggcaataact ggagcccggc gctgaacata tcgcaaggtt
     6961 taggcgatga cttcacgctg aaaatgggca ttgcccgcgc ctataaagcg ccgagcctgt
     7021 accagactaa cccgaactac attctctaca gtaaaggtca gggctgctat gccagcgcgg
     7081 gcggctgcta tctgcaaggt aacgatgacc tgaaagcaga aaccagcatc aacaaagaga
     7141 ttggtctgga gttcaaacgc gacggctggc tggcgggcgt cacctggttc cgtaacgatt
     7201 atcgcaataa gattgaagcg ggctatgtgg ctgtagggca aaacgcagtc ggcaccgatc
     7261 tctatcagtg ggataacgtg ccgaaagcgg tggttgaagg tctggaagga tcgttaaacg
     7321 taccggttag cgaaacggtg atgtggacca ataacatcac ttatatgctg aagagtgaaa
     7381 acaaaaccac gggcgaccgt ttgtcgatca tcccggagta tacgctgaac tcaacgctga
     7441 gctggcaggc acgagaagat ttgtcgatgc aaacgacctt cacctggtac ggtaagcagc
     7501 agccgaagaa gtacaactat aaaggtcagc cagcggttgg accggaaacc aaagaaatta
     7561 gtccttacag cattgttggg ctgagcgcga cgtgggatgt gacgaagaat gtcagtctga
     7621 ccggcggcgt ggacaatctg ttcgacaaac gtttgtggcg tgcgggtaat gcccagacca
     7681 cgggcgattt ggcaggggcc aactatatcg ccggtgccgg ggcgtatacc tataacgagc
     7741 cgggacgtac gtggtatatg agcgtaaaca ctcacttctg atgctaatgt cagattgttg
     7801 acaaagtgcg cgtcgttcat gccggatgcg gcgtgaacgc cttatccggc ctacaaaaat
     7861 cttgccaatt caatatattg caggaccatg taggcctgat aagcgtagcg catcaggcag
     7921 ttttgcgttt gtcatcagtc tcgaatatgg tcgatatgaa aactacgcat acctccctcc
     7981 cctttgccgg acatacgctg cattttgttg agttcgatcc ggcgaatttt tgtgagcagg
     8041 atttactctg gctgccgcac tacgcacaac tgcaacacgc tggacgtaaa cgtaaaacag
     8101 agcatttagc cggacggatc gctgctgttt atgccttgcg ggaatatggc tataaatgtg
     8161 tgcccgcaat aggcgagcta cgccaaccgg tgtggcctgc ggaggtatac ggcagtatta
     8221 gccactgcgg ggctacggca ttggccgtgg tttcccgtca accgattggc gttgatatcg
     8281 aagaaatttt ttctgcgcaa acagcgaccg aattgacaga caacattatt acaccagcag
     8341 aacacgaacg actcgcagac tgcggtttag ccttttctct ggcgctgaca ctggcatttt
     8401 ccgccaaaga gagcgcattt aaggcaagtg agatccaaac tgatgcaggt tttctggact
     8461 atcagataat tagctggaat aaacagcagg tcatcattca tcgtgagaat gagatgtttg
     8521 ctgtgcactg gcagataaaa gaaaaaatag tcataactct gtgccaacac gattaattga
     8581 caacatctgg tacgattcgc ccgcagccat cactgactgc gggcgaaagt gtaaagcagg
     8641 tgccttacca ctctgacctg acaaccggat atgcggggat tgctccccgc acaacggcta
     8701 cttcttcggt tcgtaagcga gaacagcctt aaactcaata ttacgttcct ttacggtgaa
     8761 ctcacacagc gactctccga ccagaagcgt aaatcccagc accgtcaaac acagcactat
     8821 gactgccaca agggcatatt tcgtcagcat gtttatattg cctccatgcg aagaagggac
     8881 taccatccga gttgttgagg ctcggagtgg cagccccagg attgatagaa atatctcctg
     8941 gggcttcatc tttccaaacc tcagagttaa cctgaaaccg gaaagcttca ggcacccgcc
     9001 gttatcttac ccttccttag caaaatagct gtttatttat acagtgaatc ctgtttcgga
     9061 cttctgtcgg caaaactcat cattgctctt aaaacgaaat cgcccgcagc catcactgac
     9121 cgcgggcgaa agtgtaaagc aggtgcctta ccatcctgac ctgacaaccg gatatgcggg
     9181 gattactccc cgcacaacgg ctacttcttc ggttcgtaag cgagaacagc cctgaactca
     9241 atattacgtt ccttcaccgt aaactcacac agcgagtctc cgaccagaag cgtaaatccc
     9301 agcactgtca aacacagcac tatgactgcc acaagggcat atttcgtcag catgtttata
     9361 ttgcctccat gcgaagaagg gactaccatc caaattgttc aggtttgggt aggtagcccc
     9421 agggttgata gaaatatctc ctggggcttc atctttccag acctcagagt taacctgaaa
     9481 ccggaaagct tcaggcaccc gcagttatct tacccttcct caataaaata gctgtttatt
     9541 tatacagtga gtcttgtttc ggacttctca ctcctcattt gatcaactga agctgagcca
     9601 gttttattct gcgcgcccca atcatcaaaa ttcttcgcca ccagccagct ctgccataac
     9661 ttgtcacatt gcaaaagtag ccaaattgct tcaggcacca tcagttcacc atcaccaaaa
     9721 aaaagaaaca ttccagaact tccgcatctc gcccagccgc tccgacttct atactgaata
     9781 gaaaacgcca acataagaga aacctatgcc attacccgat tttcatgttt ctgaaccttt
     9841 taccctcggt attgaactgg aaatgcaggt ggttaatccg ccgggctatg acttaagcca
     9901 ggactcttca atgctgattg acgcggttaa aaatgagatc acggccggag aggtaaagca
     9961 cgatatcacc gaaagtatgc tggagctggc gacggatgtt tgccgtgata tcaaccaggc
    10021 tgccgggcaa ttttcagcga tgcagaaagt cgtattgcag gcagccgcag accatcatct
    10081 ggaaatttgc ggcggtggca cgcacccgtt tcagaaatgg cagcgtcagg aggtatgcga
    10141 caacgaacgc tatcaacgaa cgctggaaaa ctttggctat ctcatccagc aggcgaccgt
    10201 ttttggtcag catgtccatg ttggctgtgc cagtggcgat gacgccattt atttgctgca
    10261 cggcttgtca cggtttgtgc cgcactttat cgccctttcc gccgcgtcgc catatatgca
    10321 gggaacggat acgcgttttg cctcctcacg accgaatatt ttttccgcct ttcctgataa
    10381 tggcccgatg ccgtgggtca gtaactggca acaatttgaa gccctgtttc gcagtctgag
    10441 ttacaccacg atgatcgaca gcattaaaga tctgcactgg gatattcgcc ccagtcctca
    10501 ttttggcacg gtggaggttc gggtgatgga taccccgtta acccttagcc acgcggtaaa
    10561 tatggcgggg ttaattcagg ctaccgccca ctggttactg acggaacgca cgttcaaaca
    10621 tcaggagaaa gattacctgc tgtataaatt caaccgtttc caggcctgtc gctatgggct
    10681 ggaaggcgtc attaccgatc cgcacactgg cgatcgtcga gcgctaacgg aagatacctt
    10741 gcgattgctg gaaaaaatcg ccccttccgc acataaaatt ggtgcatcga gcgcgattga
    10801 ggccctgcat cgccaggtcg tcagcggtct gaatgaagcg cagctgatgc gcgatttcgt
    10861 cgccgatggc ggctcgctga ttgggctggt gaaaaagcat tgtgagatct gggccggtga
    10921 ctaagccggg aattgcctta cagaagaata tccctgacaa tggtgtttta acttacattt
    10981 aacaatgtta tgaaacaccc tttagaaacc ttgaccaccg cagcaggcat tttgctgatg
    11041 gctttcctct cttgcctgct gctgcccgcc cccgcactgg ggctgacgct ggcacaaaaa
    11101 ctggtgacca cgttccatct gatggatctt agtcagcttt acactttatt gttttgtctg
    11161 tggtttttag tgctgggcgc tattgagtat tttgttctgc gctttatctg gcgacgctgg
    11221 ttctcgctgg cggattaaac gtcgattatc gccgtacttc gcggcatact ttgcttattc
    11281 tccttcgcct aaaggaacgt ttatggataa gcaatcactg cacgaaacgg cgaaacgcct
    11341 ggcccttgag ttaccctttg tcgagctttg ctggcctttt ggcccggagt tcgatgtttt
    11401 taaaattggc ggcaaaattt ttatgctgtc gtcggagctg cgcggcgtcc cctttatcaa
    11461 tctgaagtcc gatccacaaa aatccctgtt aaatcagcag atatacccga gcattaagcc
    11521 agggtatcac atgaataaaa agcactggat ttcggtgtat cccggcgagg aaatctccga
    11581 agcgttactt cgcgatctga tcaacgattc gtggaatctg gtggttgatg gtctggctaa
    11641 acgcgatcaa aaaagagtgc gtccaggcta aagcggaaat ctatagcgca tttttctcgc
    11701 ttaccatttc tcgttgaacc ttgtaatctg ctggcacgca aaattacttt cacatggagt
    11761 ctttatggat atcatttctg tcgccttaaa gcgtcattcc actaaggcat ttgatgccag
    11821 taaaaaactt accccggaac aggccgagca gatcaaaact ctcctgcaat acagcccatc
    11881 cagcaccaac tcccagccgt ggcattttat tgttgccagc acggaagaag gtaaagcgcg
    11941 tgttgccaaa tccgctgccg gtaattatgt gttcaacgaa cgtaaaatac ttgatgcctc
    12001 gcacgtcgtg gtgttctgtg caaaaaccgc gatggacgat gcctggctga agctggttgt
    12061 tgaccaggaa gatgctgatg gccgctttgc cacgccggaa gcgaaagccg cgaacgataa
    12121 aggtcgcaag ttcttcgctg atatgcatcg taaagatctg catgatgatg cagagtggat
    12181 ggcaaaacag gtttatctca acgtcggtaa cttcctgctc ggcgtggcgg cgctgggtct
    12241 ggacgcggta cccatcgaag gttttgacgc cgccatcctc gatgcagaat ttggtctgaa
    12301 agagaaaggc tacaccagtc tggtggttgt tccggtaggt catcacagcg ttgaagattt
    12361 taacgctacg ctgccgaaat cacgtctgcc gcaaaacatc accttaaccg aagtgtaatt
    12421 ctctcttgcc gggcatctgc ccggctattt cccctcagat tctcctgatt tgcataaccc
    12481 tgtttcagcc gtcatcatag gctgctgttg tataaaggag acgttatgca ggatttaata
    12541 tcccaggttg aagatttagc gggtattgag atcgatcaca ccacctcgat ggtgatgatt
    12601 ttcggtatta tttttctgac cgccgtcgtg gtgcatatta ttttgcattg ggtggtactg
    12661 cggaccttcg aaaaacgtgc catcgccagt tcacggcttt ggttgcaaat cattacccag
    12721 aataaactct tccatcgttt agcttttacc ctgcagggga ttatcgtcaa tattcaggcg
    12781 gtattctggc tgcaaaaagg caccgaagcg gcagatattc tgactacctg cgcgcagttg
    12841 tggatcatga tgtatgcgct gctttcagtc ttctcgttgc tggatgttat tttgaatctg
    12901 gcgcagaaat tcccggcggc atctcagtta ccgctgaaag ggatatttca ggggattaaa
    12961 ctgatcggcg cgattctggt cggcattttg atgatctcgc tgctgattgg tcagtcgcca
    13021 gcgattctga tcagcggtct tggtgcaatg gctgccgtgc tgatgttggt atttaaagat
    13081 ccgattcttg gtctggtggc aggtattcag ctttccgcga acgatatgct gaaactgggc
    13141 gactggctgg agatgccgaa atacggcgcg gatggcgcag tgatcgatat tgggttaacc
    13201 accgtcaaag tgcgtaactg ggacaatacc attaccacta ttcccacctg gtctctggtc
    13261 tctgactcct ttaaaaactg gagcgggatg tcagcatctg gcgggcgacg tattaagcgc
    13321 agtatcagta ttgatgtcac cagtattcgt tttcttgatg aagacgaaat gcaacgtctg
    13381 aataaagcgc atttgttaaa gccttattta accagccgcc atcaggaaat taatgagtgg
    13441 aatcgccagc aaggttctac ggagtcggta ttaaacctgc gccgaatgac caatattgga
    13501 acctttcgtg cctatctgaa cgaatatctg cgtaaccatc cgcggattcg taaagatatg
    13561 actttaatgg tacgccaact ggctccaggt gataacggtt taccgctcga gatctatgcg
    13621 tttaccaaca ccgtggtgtg gctggaatat gaaagcattc aggctgatat attcgatcac
    13681 atatttgcga ttgtcgaaga gtttggtctg cgacttcatc agtcgccaac cggcaatgat
    13741 attcgctctc tggcgggtgc atttaagcag taattaaaaa aaccgctctc atcgaatgga
    13801 tgagagcgga catcgttttg tccgatgtag cgtgaatgcc ttatttccga cgcagcgttt
    13861 taaatgccac aaacaggaat acaatccaca ccggcagcag gatcgctgac aagcgcatat
    13921 catccatcgt gcacatcagc agcaaaatca tgccgaggaa ggcaatgcaa agatagttgc
    13981 cgaacggata aagcagcgcc ttaaactgtg tttcacgtcc ctgacgtcgc atcgccgcgc
    14041 gaaaacgcag atgcgccagg cagatcataa tccagttcaa cagcagcgtt gctaccacca
    14101 gcgccatcag cagaccaaac gctttttgcg gcagcagata gttgattaac accaccagcg
    14161 aagtgatcgc tccggaaagc atcagcgagt taatcggcac accgcgacgg ctgacgcgag
    14221 tcaaaaactt cggcgcatta ccctgcacag aaaggccaaa cagcatgcgg ctgttagagt
    14281 aaaccccgct gttatacact gacagcgatg cgaccagaat gacgaagttc agcgcagaag
    14341 ctaccacgtt gctgtcgaga ttatggaaaa tcatcacaaa cgggctactg ttggatttca
    14401 cttccaccca cggatagagc gccagtaaaa ccaccagtga accgatgtaa aacagcagga
    14461 tgcgatacac cacctgattt accgcttttg gaatgctttt ttccggatcg cgcgcttcag
    14521 cggcagtaat cccaatcagc tccagaccgc cgaaggagaa cataattacc gccagcgaca
    14581 aaatcagccc attccagccg gtggcgaaga aaccaccgta gcgccagagg ttgtcgatac
    14641 tggctttctc gccgccgtga ccagaaaaca gcagccacag gccaaagccg atcataccga
    14701 tgatcgccag cactttaatc agcgcaaacc agaactcggt ttcgccatat aagcgcacgt
    14761 tcaccaggtt aacggcgttg atgataataa agaaggcggc agcccaaatc cacgttggaa
    14821 catccgggaa ccagtactgc atatagatgc ccgcagcggt cagctctgcc attcccacca
    14881 gcacgaacat tacccagtag ttccagccag agaggaagcc cgcaaacggt ccccagtatt
    14941 tataggcaaa gtgggcaaat gaaccggata ccggctcttc gaccaccatc tcgccgagct
    15001 ggcgcataat caggaaagcg atgatcccgg cgacgccgta gcccagcaat acagccggac
    15061 ccgccatctg aatcgccggg ccaatgccaa gaaacagacc agtaccaatt gcgccaccca
    15121 gcgcaatcag ttgaatatga cggttatgta atccgcgatg aagcgtcggc tcttgattcg
    15181 acgcagtatc ttccgatacg gttgacgcgt ttttcacgcc tttcccctgt gtgtcttttt
    15241 tgttgagggg caccttttaa catttagtgc ccatcgtcgc aagacacaat ccacacggtt
    15301 aaacggggta tcctgctttt atttccgtac ccgatgtcgg tgcagccaca tcagcttata
    15361 cgccgccggg ataataaaca gcgacagcaa aggtgcggtg atcatgccgc caatcatcgg
    15421 cgcggcaatc cggctcatca cctctgatcc tgcccccgtt ccccacagaa tcggcagcag
    15481 accggcgata atcaccgcca ccgtcatcgc tttcgggcgc acgcgcagga ccgcgccgtg
    15541 atataacgcc tcatccagct tctgctcgct gaatgtttgt ggattattca acgacggctc
    15601 ggcctctatg gcgtgacgta aatacatctg catcaccacg ccaaattcgg cggcgacccc
    15661 ggcgagggcg ataaagccag tgcccgtcgc cacagaaaga tgaaagccca tccaccacag
    15721 caaccagatg ccgcccacca gcgcaaacgg tacgctgctg ataatcagca acgcttcgcc
    15781 cacccgacgg aacgccagat acaacagcac gaagataatc atcaacgtca tcggcaccat
    15841 gagtttcagc ttatggttgg cgcgctccag tagctcgaac tgcccggaga atgccacgct
    15901 ggtgcccggt tttaactgca ctttttcagc tatcgctttt tgcaaatcgt gaaccaccga
    15961 caccatgtca cgatcgcggg catcgatata aatccagctc gtcgggcgcg cattctcggt
    16021 tttcagcatc gacggtccgg tagagacttt aacgtcggct acgtctgcca gggtgatttg
    16081 ctgcttcatc ggcgtcagga tcggcaactg gcgcagtgcc tgcggactat cgcgccagct
    16141 ttgcggataa cgcagattaa tgggataacg ggcaatccct tccaccgttt cgccaaccat
    16201 cgccccgccc accgcagaag tcacaaacaa ctgcacatcc gccaccgtca taccgtaacg
    16261 cgcggctttt tcacggttaa tctcaacgtt gatatagcgc ccaccttcca gacgctcggc
    16321 aagcgcagaa gccacgcctg gcaccgttcg tgccacctct tcaatttgct ccgccatcgc
    16381 gtcgatatcc gccagcacag tgccggaaac ttttatgccg atagggcttt taatgccggt
    16441 tgagagcata tcgatacggt tacgaattgg cggcacccac agattcgcca gccccggcag
    16501 acgcacggtg ttatccagtt cctcaatgat tttgtccatt gtcatgcctg gccgccactg
    16561 ctcctgcggc ttaagctgga tggtcgtttc caccatctcc agcggagcag agtcggtggc
    16621 ggtttccgct tttccggttt tgccaaatac ccgcgccact tcaggtacgc tcataattag
    16681 cttgtcggtt ttttgcagca tactcgccgc ctctgctgcg gaaatccccg gcaacgtcga
    16741 tggcatatac aacaagtcgc cttcattgat ctgcggtaaa aattccccgc caactttatt
    16801 gagcggccag agaaccgtca gcaccgaaag cgctgccacc aacagcgtgg ttttcggcca
    16861 gtgcagtact ttcagcaata gaggatgata aacacgaatc aaaaagcgat tgagcgggtt
    16921 actgctttcc ggcggaattt tgccacggat ccagtaaccc atcaggatag ggatcactac
    16981 gatcgccagc agcgccgcac ccgccatcgc ataggtttta gtgaacgcca gcgggccaaa
    17041 cagacgccct tcctgccctt ccagggtgaa gatcgggata aacgacagcg tgataatcag
    17101 cagactgata aacagcgccg gccccacttc aacagatgca tcggtaatca cctgccagcg
    17161 cgttttatta tccagcgtgg cgtcaggatg ctggtgctgc cactcttcca gccgtttatg
    17221 tgcattctcg atcatgacga tagcggcatc gaccatcgcc ccgacggcaa tcgcaatgcc
    17281 acccaacgac ataatattgg cattcagccc ctggaagtgc atgacaataa aagcaataca
    17341 caaccccagc ggcaacgaaa taatcgccac cagcgccgag cgcacatgcc agagaaacag
    17401 cgcacagact accgccacca caataaactc ttccagcaac ttgccgctga ggttgtcgat
    17461 ggcccggtca atgagctggc tgcgatcgta tgttgtaact atctccacgc cttccggcag
    17521 actacttttc agcgtttcca gtttgtcctt cacggcggcg atcacttctc gggcgttttt
    17581 gccggagcgt aggatcacca ccccgcccgc aacttcgcct tcgccgttca gttcggcaat
    17641 gccccggcgc atctccgggc caacctggac cttcgcaaca tcacgcagat aaacgggcac
    17701 gccattttca ctggctttta aaacgatgtg attaaagtcg tcgagcgttt gcagatagcc
    17761 gctggcgcgc accatatatt ccgcttccgc cagttcgatc gacgaaccgc ccgcttcctg
    17821 gtttgaagca tccagcgcat ttttcacttc gccgagactg atgccatact gcgccaggcg
    17881 ctgaggatcg ataaccacct gatactcttt caccacaccg cccaccgacg ccacttccgc
    17941 aacgtcaggg atggttttta gctcatattt gagaaaccag tcctgcaatg agcgtaaatc
    18001 ggcaagatcg tgcttaccgc tgcgatccac cagtgcatat tcatagatcc agccaacacc
    18061 cgtggcatct ggccccagct cggcgctgac tcctgcaggc agcttaccct gtacctggtt
    18121 gaggtactcc agcacccgcg aacgcgccca gtacggatcg gtgccatctt cgaaaatgac
    18181 atacacataa gagtcgccaa actgcgagaa accacgcaca gtcttcgcgc caggcaccga
    18241 caacatggtg gtagttagcg gataagtcac ctgattttca acgatttgcg gtgcctgacc
    18301 gggatagctg gttttaataa tcacctgcac atcggagaga tccggcaacg catccactgg
    18361 cgtgttaatg atggtccagg tgccccagat actcagaaat aacgcgccca tcagcaccag
    18421 aaaacggttc gccaccgagc gacgaataat ccattcaatc attggttatt ccctcaatgc
    18481 gcatgggtag cactttcaga gcgcatccgc tccagtgcgc cagaaatatt ggcttcggaa
    18541 tcaatcagga acaggccgct ggaaaccacc ttttcacctt ccgccagacc agagcgtaat
    18601 gcggtgacac cttgcgatgc ctggaaaacg gcaacgcgtt ttggtacaaa gcgcccgtcg
    18661 gcatcaacgg taatcacccg ctgttcgctg ccggtatcaa tcagcgcttg tgacggaatg
    18721 agcagcatcg gttcgctggc ggtattgagt tgcaaccagg cgttcattcc cggttttagc
    18781 gcctcgtcgg cgttgtcgac ttccagacgc agctgcaacg tgcgggtcgc ggcatccacg
    18841 ccaggtagca gcgtccattt gcggatggtg agtgttttat ccggtcgtgc cggaacggtg
    18901 agcgtaaact gcgaggcatc tttcaccagc caggcgatgg actccgggat cgcagcagtg
    18961 acccacaccg ggtccatacc ctgaattttc gcgaccacgt tatctttggc gatattcatc
    19021 cccgcacgca gatcaaacgc ggtgatcacg ccatcaatgg gcgctttgag cgtaaagcga
    19081 gtctggattt tttgcgtggc gctcaggcgg cgaatatccg cctccggcat tccagccagt
    19141 cgcagccgct caagaatgcc ttcagtctgg gtcgccgtac cgccggtttc gcgcagcagt
    19201 aaatactcac tctgcgcttc cacccagtcg ggaatggtca ggtcgagaag cggtgtgccc
    19261 ttttgcactt tatcgcccac ggtaagcgga tacaccttgt cgataaaccc ggcagcgcga
    19321 gcctgcacaa tggcatactg atactcgttg taactgacat tcgccgggaa actctgggca
    19381 aaagtcagcg gcccgcgcgt gacggtagcc gttttcaccc ccagattctg cgtctgagtc
    19441 gggtcaatgc gcacaccaga cgcagaactc tcttcatcgg catatttcgg caccagatcc
    19501 atatccataa acggcgattt acctggttta tcgaaccgcg tattgggata catcgggtcg
    19561 taccagaata agactttacg ttctgcggtc gacgtttttt ctgcgggcgg ttcagcccgg
    19621 tcaaaccagg taaaacctac cgcagaaata ataccgcccg cgatcatgct gccgataata
    19681 agcgcgattt ttttcatctc attaaacctg ggttactggc tgactttaat atcctgtaat
    19741 aaagaaaggt tgccctgctg gacaaaatta aacgccactt tgtcgccggt tttaatttca
    19801 ctcattttcg tctgcggggt gatggtaaag cgcatggtca tctccggcca gttcacggca
    19861 gcaatcggat cgtgatggat ggttattttt ttgctttcca gatcaatacc ttttaccacg
    19921 ccggaggcgc taataacctg tggttgtgct tcgctcatgg tttcatgatg atgttcgtta
    19981 gcctgggcat taaagccaat aacggtaaac agactgaaca ttgcgacttg cagtgctttt
    20041 ttcatttatt ctctcctgga gttaaaaatt actgctgcca accgccccct aaggcggtat
    20101 ataaagaaat ttcgttaacc tgacgggcat aattcaggtc aagtaaagtt tgtcgggttg
    20161 caaataaaga acgctcggca tccagcactt ccagataact taccgcgccg tgctgatata
    20221 atgcccgcgc ccgttgcaaa gtaattttca gcgacgccag ataacgctgc tgggcgctaa
    20281 tttgatcgtt caggctttga cgtaatgcca gcgcatctgc cacttcttta aaggcgttct
    20341 ggattttctg ttcataattc accaccgact gctgctggcg aatttcggcg atatccagat
    20401 tggcctggtt gcgtccggca ttaaaaatgg ggatctcaat tttgggtata aaattccaca
    20461 tcccgctgct ggcgttaaat aatgacgaca gatcgctgct ggcggttgat attccgctgg
    20521 tcaggcttat agatgggaaa aatgccgcgc gtgcggcccc aatattggca ttagccgcca
    20581 ttaacgcgtg ttcagcttcc ataatatccg ggcgctgcaa taagatttgc gacgacaagc
    20641 ccgccggtaa tttaacgctt tgcaggctgt cgctgtttac tgtctgcgct tgcggcagct
    20701 tgccgtagct tcccaataac agttgcaatg cattattcgc ctgtgccagt tccccctgac
    20761 gtttagcgat gtcactgcgg gtactttcta tcaccccgcg agcctgttcc agcgccagaa
    20821 cattgctgct accggtcaac agttgttttt cgacaaacgc atatgactgc tgataattac
    20881 gcagcgtttc ttcggctatt tgcaattgcg catacgccag ttgctgattg aaatagcttt
    20941 gcgcgacatt agaaaccagc agaatatgca ccgcgcgctg agcttcctca gtggctaaat
    21001 aattttgtcg ctcggcttcg ctcatgttct ttaagcgccc gaaaaaatcg aggtcaaagc
    21061 tggcgttaag gccagtagaa aactcccgcg tcgtggctga atcgccttta agattgccgc
    21121 tccagctgcc gctgccctcg ccattgagct gtgggtagcg gtcggcatcg gtcagacgat
    21181 attgcgcccg tgcctcctgc actttcagcg tcgccatgcg caaatcccgg ttattcacca
    21241 gcgcctcgct aatcagcgtc ttcacctgat tatcaacaaa aaaggtgcgc cagtccgcgt
    21301 tctgatagtt atctgctgcg ttaaccaggc cgttctggct gagtgagaac tgctgcggta
    21361 cgggcattgc cgggcgctga taatccggtg ccagtgaaca accggttagc gcaagggcca
    21421 cacaaaatgg cagaagttta caaggagaca taggctcata atttctggtg attttatacc
    21481 gccaacttta ctcgccaggc tctgattttc cggtgacagg aaaatgacaa aattgtcatt
    21541 ttgccaataa gcgattgcca tctgatcccg ctactctaga attgcccggg caacatgtgg
    21601 aggaaatatg aaactgttga ttgtcgaaga tgaaaagaaa accggagaat acttgaccaa
    21661 agggttaacc gaagccggtt ttgtggtcga tttggccgac aacgggctga atggctacca
    21721 tctggcgatg accggagatt atgacctgat aatcctcgat attatgctgc cggacgtgaa
    21781 cggctgggat atcgtgcgca tgttacgttc cgccaataaa gggatgccga ttctgttgct
    21841 taccgcgctt ggcaccattg aacatcgcgt caaggggctg gagttggggg cagatgacta
    21901 cctggtgaag ccattcgctt ttgctgaact gctggcgcgg gtgcgcacat tactgcggcg
    21961 cggggcggcg gtgattatcg aaagtcagtt tcaggttgcc gatttgatgg tcgatctcgt
    22021 cagccgcaaa gtcacccgca gcggtacgcg catcactctg accagtaaag agtttactct
    22081 gctggagttc ttccttcgcc atcagggcga agtgctgccc cgctcgctta tcgcctcgca
    22141 ggtatgggac atgaatttcg acagcgacac taacgccatt gatgtggcgg tgaagcggct
    22201 gcgcggcaaa atcgacaacg actttgagcc gaagctaatt cagaccgtgc gcggcgtggg
    22261 ctacatgctt gaggtgccgg atggtcagta agccatttca gcgcccgttt tcgctggcaa
    22321 cccgcctgac cttttttatc agcctggcca ccatcgcggc gtttttcgcc tttgcatgga
    22381 tcatgatcca ctcagtaaaa gtgcattttg ccgagcagga tattaatgat ttaaaagaga
    22441 ttagcgccac ccttgaacgg gtactaaatc accctgacga aacgcaagcc cgacgcttaa
    22501 tgacgctgga agatatcgtc agtggttatt ccaacgtgtt gatttccttg gcagatagcc
    22561 acggcaaaac ggtgtatcac tcccccggtg cgccggatat ccgcgagttt acgcgtgacg
    22621 ccatacccga taaagacgct cagggtggcg aggtgtatct cctttccggc ccgacgatga
    22681 tgatgccagg ccacggtcac gggcatatgg aacacagcaa ctggcggatg atcagtttgc
    22741 cagttggccc actggttgac ggcaaaccga tttatacgct ctacatcgcg ctttcgatcg
    22801 attttcatct tcattacata aatgatttga tgaataaact tattatgacc gcatcgatca
    22861 tcagcatcct gatcgtcttt atcgtgctat tggcggtaca taaaggtcac gcgccgatcc
    22921 gcagcgtcag ccgtcaaatc cagaatatca cctcgaaaga tctcgacgtt cgccttgacc
    22981 cgcagaccgt gcccattgag ctggaacagc tggtactgtc gttcaaccat atgatcgagc
    23041 gtattgagga tgtctttacc cgccagtcca atttctcagc ggatatcgct cacgaaattc
    23101 gcacaccgat taccaatctc atcacgcaaa cggaaatcgc cctcagccag tctcgcagcc
    23161 agaaggagct ggaagatgtg ctctactcta atctcgaaga gctgacgcga atggcgaaaa
    23221 tggtcagcga tatgctgttt ctcgctcagg ccgataacaa ccagctaatc cccgaaaaga
    23281 aaatgctcaa cctggcggat gaagtcggca aagtgttcga ttttttcgag gctttagcgg
    23341 aagatcgcgg cgtggagctg cagtttgttg gcgacgagtg ccaggttgca ggcgatccgc
    23401 tgatgctgcg tcgggcatta agcaacctgc tttctaatgc cctgcgttat acgccacccg
    23461 gagaggctat tgtagtgcgc tgccagacgg tcgagcatca ggtgcaagtt atcgtcgaaa
    23521 accccggtac tcccattgcg cccgagcact taccgcgatt gtttgaccgt ttctaccgcg
    23581 ttgacccttc ccgccagcga aaaggcgaag gtagcggcat tgggctggcg atagtgaaat
    23641 cgattgttgt cgcacataaa ggcacggttg cagtaacgtc agatgcgcgg gggacaaggt
    23701 ttgtgattgt gttgccggag agggggtgat tattaagttc aaattcaggg cgagggttta
    23761 cggcgttttt cgctaa
//
LOCUS       DAPGOF010000070        23448 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.070, whole genome shotgun sequence.
ACCESSION   DAPGOF010000070 DAPGOF010000000
VERSION     DAPGOF010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.070"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            150..689
                     /locus_tag="RHV13_002365"
     CDS             150..689
                     /locus_tag="RHV13_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HDV1241892.1"
                     /translation="MELLMSNVSGIGDAYYWSVFKIAEAFGLHRDTVKKRLLAANTPV
                     AATVRGNPVYALQHVGPALFSVKHEAADSVHDPSRMEPKERKDWYQSENERIKLEKEQ
                     RKLIPVDEVVIVYSSMRKAVVQVLETIPDVLERDCALTPQAVGVVQQAIDDLRYTLQE
                     KSYEACAAELIPDEEGESL"
     gene            698..2797
                     /locus_tag="RHV13_002366"
     CDS             698..2797
                     /locus_tag="RHV13_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015987600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HDV1241893.1"
                     /translation="MGFSSARNLGRDISAGFSPPRRMPISEAVKKFMRVPKGAGNSVP
                     WDPELTPYIIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWIVYTIVCDPSDMLVVQ
                     MTEDKAREHSKKRLDRTFRSSAAVKKRMSPRRNDNNVHDKTFRDGSFLKIGWPSVNIM
                     SSSDYRFVALTDYDRFPENIDSEGDGFSLASKRTTTFMSAGMTLVESSPGRDICDSKW
                     RRKSPHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPAMDAMTGYRNEPDPFKASE
                     AAYLLCPHCSGIITAEKKRELNSAGVWLREGQVIDRNGNVSGEPRRSRIASFWMEGPA
                     AAYQTWAQLVYKLLTAEQEYEATGSEETLRAVINTDWGLPYLPRASMEQRKSELLEQR
                     AEPVPSRSVPDGVNFLVATVDVQAGRHRRFVVQVTGYGSRGERWIIDRYNITQSLRSD
                     CDGESQRIDPASYPEDWDVLLTDVFHKSWPLASDPSQQMRLMAMAVDSGGEDGVTDNA
                     YKFWRRCRRDGLGKRIYLFKGDSIRRAKLISRTFPDNTGRTGRRAQAAGDVPLWLLQT
                     DALKDRVNNALWRDSPGPGYVHFPDWLGSWFYDELTYEERSSDGKWSKPGRGANEAFD
                     LMVYAEALVILHGYEKIRWPDAPEWASRETWLECVPDSTEPSPSPEPVPTPVKKQKRK
                     KTVTDDVNPWLTSGGWL"
     gene            2794..3006
                     /locus_tag="RHV13_002367"
     CDS             2794..3006
                     /locus_tag="RHV13_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1241894.1"
                     /translation="MNQNDIEAMIQRYTEAEMAVLDGKSVTFNGQQMTMENLSEIRQG
                     RQEWERRLAALITRRRGHPGYRLARF"
     gene            3006..4514
                     /locus_tag="RHV13_002368"
     CDS             3006..4514
                     /locus_tag="RHV13_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDV1241895.1"
                     /translation="MAILDDVIGVFSPRWKAARLRSRAVIQAYEAVKTTRTHKARRES
                     RTADQLSQYGAVSLREQARYLDNNHDLVIGVFDKLEERVVGKNGIIVEPHPVLRNGAI
                     ARDLAAEIRTRWSEWSVSPEVTGQFTRPMLERLMLRTWLRDGEVFAQMVSGRINSLTP
                     SAGVHFWLEALEPDFIPMTSDESNRLNQGVFVDDWGRPEKYLVYKSRPVSGRQMETKE
                     VDAERMLHLKFVRRLHQMRGTSLLSGVLIRLSALKEYEDSELTAARIAAALGMYIRKG
                     DGQSYETDGNDSKENERELTIQPGIIYDDLKPGEEIGMVKSDRPNPNLETFRNGQLRA
                     VAAGSRLSFSSTARNYNGTYSAQRQELVESTDGYLILQDWFIGAVTRPMYRAWLKQAV
                     ASGVIRLPRDLDRSSLYTAVYSGPVMPWIDPVKEAEAWKIQIRGGAATESDWVRAGGR
                     NPDDVKRRRKAEIDENRKLDLVFDTDPASDKGGSSAATKRQEPQHTDDQSEE"
     gene            4459..6486
                     /locus_tag="RHV13_002369"
     CDS             4459..6486
                     /locus_tag="RHV13_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HDV1241896.1"
                     /translation="MPQRNDRSRSTPTTSPKNNSWFRMQAGHQSDADIYIYDEIGFWG
                     VTAKQFISDLNALGDITHINLHINSPGGDVFEGIAIFNALKTHGASITVYVDGVAASM
                     ASVIAMVGNPIIMPENSFMMIHKPFGFTGGDAEDMRTYADLLDKVEAVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAQGFADQVTPAVKAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRVQDDEPAASRTPVQAAAPVVDENSIRAQVLAEQKARVNGINDLFAMFG
                     GRYQMLQAQCLADPECSLEQAREKLLNEMGRESTPSNKNTPAHIYAGNGNFVGDGIRQ
                     ALMARAGFEKTERDNVYNGMTLREYARMSLTERGIGVSGYNPMQMVGAAFTHSTSDFG
                     NILLDVANKAILQGWEDAPETYEQWTRKGQLSDFKIAHRVGMGGFSALRQVREGAEYK
                     YVTTGDKQATIALATYGELFSITRQAIINDDLNMLTDVPMKLGRAAKSTIADLVYAIL
                     TSNPKISTDNVSLFDKAKHANVLESAAMDVASLDKARQLMRVQKEGERHLNIRPAFVL
                     VPTAMESVANQVIRSSSVKGADINAGIINPVKDFATVIAEPRLDDNSQTTFYLAASKG
                     SDTIEVAYLNGVDTPYIDQMEGFSVDGVTTKVRIDAGVAPVDHRGLVKCTA"
     gene            6573..6896
                     /locus_tag="RHV13_002370"
     CDS             6573..6896
                     /locus_tag="RHV13_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HDV1241897.1"
                     /translation="MAKNFVEEGKTVAIVASAAISSGDLVQVGDVFAVALTDIPQGET
                     GDGMTEGVFMLPKLKTDDMKTGKKVYLKSGKVQLTNSGSDPLVGVVWADAGTSAEEVP
                     VKLNV"
     gene            6889..7164
                     /locus_tag="RHV13_002371"
     CDS             6889..7164
                     /locus_tag="RHV13_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HDV1241898.1"
                     /translation="MSDPFSRLAARMDAITVRKMGKTASINDVDMTVIPGETLAELNA
                     LSGPAVSLVVFSSGYRPRRGDRVVYDGQQWTVTRHERFNGKPMIFIE"
     gene            7176..7754
                     /locus_tag="RHV13_002372"
     CDS             7176..7754
                     /locus_tag="RHV13_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV1241899.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLGTARVRLARR
                     GGKLQYRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKVPLSGPLTQ
                     AFEDARDRIIAAEMPKQLGYALKQQLRLWLTR"
     gene            7751..8152
                     /locus_tag="RHV13_002373"
     CDS             7751..8152
                     /locus_tag="RHV13_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV1241900.1"
                     /translation="MNRHTQIRQAVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDIPALSGLID
                     TLIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            8164..8907
                     /locus_tag="RHV13_002374"
     CDS             8164..8907
                     /locus_tag="RHV13_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV1241901.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGKGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVS
                     VTGLTVAPASASVKVGATTTLTFTVKPDGASDKAISVHSTDPQTATVTLNGLVATVKG
                     VKQGSVSIVGMTSDGDFVAVAAVAVSAAG"
     gene            8968..9354
                     /locus_tag="RHV13_002375"
     CDS             8968..9354
                     /locus_tag="RHV13_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HDV1241902.1"
                     /translation="MFLKQGTFNYEKQSVVLSELSGLQRIEYLAFVQQRTAKFDAEEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASVITTWSYDALGAGAEM
                     VLSLSGMGVIENAGDLEHEVLTPEKS"
     gene            9363..9692
                     /locus_tag="RHV13_002376"
     CDS             9363..9692
                     /locus_tag="RHV13_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HDV1241903.1"
                     /translation="MQFVMRLAREFRRADWRRMLSEMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            9664..12729
                     /locus_tag="RHV13_002377"
     CDS             9664..12729
                     /locus_tag="RHV13_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDV1241904.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTTQAASSAATAASVHAQTVEKNAQAHERMAREVEKTRQRMEALSQK
                     MREEQAQAMALAEAQDKAAAAFYRQIDSVKQASAGLQELQRIQQQIRQARNSGGIGQQ
                     DYLALISEVTAKTRVLTQAEEEATRQKVAFIRQLKEQATRQNLSSSELLRAKAAQLGV
                     SSAAEVYIRKMEQAGKATHSLGLKSAAARQEIGVLIGELARGNLGALRGSGITLANRA
                     GWIDTLMSPKGMMLGGVIGGIAAAVYGLGKAWYDGQKEGEEFNRQLSLTGHYAGVTAG
                     QLWTLSRAISGNGITQHAAAGALAQVVGSGAFRGNDIGMVARAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNELHFLTATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     RRTADIDNNLNALGSTLKYLSDLWSRFWDAAMNIGREDSLDEQISALQEKVSRAKRLP
                     WTASSSQVEYDQQRLNELQKKKRQKDLQDAKEQAEQNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINSMQYADQAVRDAAIQRENERYEKALASGKKKTREPRNDEATRLLL
                     QYSQQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLA
                     RKDELIQALTLLDVKQQELQKQTALNELKKKTIQLTSQLAEEERAQRQQHDLDIATVG
                     MGDQQRQRYQVQLSLRQKYQQQLEQLRRDSEQKGTYNTDDYRKAEQALTESLNRQLNE
                     NRRYWQQLEVVQGNWKNGVLRAFQNFTVDADNTAGTAEQVFSSAFSNMGNGLATFVTT
                     GKLNFKSFTSSVLSDMAKILAQATMMKSIKGIGSVLGFDLSSLSLNANGGIYQSADLS
                     RYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADIGGSGMAMFAPQ
                     YNIEINNDGTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            12729..13058
                     /locus_tag="RHV13_002378"
     CDS             12729..13058
                     /locus_tag="RHV13_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV1241905.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSIY
                     SVTIRVRKCEHPSLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            13068..13766
                     /locus_tag="RHV13_002379"
     CDS             13068..13766
                     /locus_tag="RHV13_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDV1241906.1"
                     /translation="MQDISQETHHETTRLTQSAQVVLWEIDLTVVGGERYFFCNEQND
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            13772..14515
                     /locus_tag="RHV13_002380"
     CDS             13772..14515
                     /locus_tag="RHV13_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV1241907.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLSAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGIYNDLAAASICV"
     gene            14452..15054
                     /locus_tag="RHV13_002381"
     CDS             14452..15054
                     /locus_tag="RHV13_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HDV1241908.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALAMQIPAFRQKLSD
                     GWYQVRIAGRDTGETELSARLNEPLANGAVIHIVPRLAGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKPKTPRTQTTDNGKQNTYFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            15115..18594
                     /locus_tag="RHV13_002382"
     CDS             15115..18594
                     /locus_tag="RHV13_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HDV1241909.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSDGNTNIAGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     AITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQNCDQSVPDGFGGTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWEAATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFMLRLTVTADDGSERLVSTARTTETTYRFRQ
                     LALGRYTLTVRAVNAWGQQGDPASVSFRIAAPAAPSQIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRPSDDASGYLDFFKGEIGKSHLAQELWTQIDNGQLAPDLAEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKYLTAPTITSGGNP
                     PAFSLTPDGKLTAKNADISGSVNANSGTLNNVTINENCQIKGKLSANQIEGDIVKTVG
                     KAFPRDSRAPERWPSGTITVRIYDDQPFDRQIVIPAVAFSGAKHEREHTDIYSSCRLI
                     VKKNGAEIYNRTALDNTLIYSGVIDMPAGHGHMTLEFSVSAWLVNDWYPTASISDLLV
                     VVMKKATAGISIS"
     gene            18662..19261
                     /locus_tag="RHV13_002383"
     CDS             18662..19261
                     /locus_tag="RHV13_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HDV1241910.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEQQATLSAGYLHARTNAPG
                     SDNLNGINVKYRYEFTDTLGLITSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGMAYSRVSTFSGDYIRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAVDVAYEGSGSGDWRTNSFIVGVGYKF"
     gene            19326..22724
                     /locus_tag="RHV13_002384"
     CDS             19326..22724
                     /locus_tag="RHV13_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HDV1241911.1"
                     /translation="MAVRISGVLKDGAGKPIQNCTIQLKARRNSTTVVVNTVASENPD
                     EAGRYTMDVEYGQYSVSLLVEGFPPSHAGIITVYEDSKPGTLNDFLGAMTEDDVRPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSAGDAGTSAREAATHATDAAGSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSASAAKTSETNAAASQQSA
                     ATSASTATTKASEAATSARDASASKEAAKSSETSAASSASSAASSATATANSAKAAKT
                     SETNARSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQASAAARSASAAKTSETNAKASETSAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKSSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSETLAATPKAVKAAYDNAEKRLQKDQNGAD
                     IPDKGRFLNNINAVSKTDFADKRGMRYVRVNAPAGATSGKYYPVVVMRSAGSVSELAS
                     RVIITTATRTAGDPMNNCEFNGFVMPGGWTDRGRYAYGMFWQYQNNERAIHSIMMSNK
                     GDDLRSVFYVDGAAFPVFAFIEDGLSISAPGADLVVNDTTYKFGATNPATECIAADVI
                     LDFKSGRGFYESHSLIVNDNLSCKKLFATDEIVARGGNQIRMIGGEYGALWRNDGAKT
                     YLLLTNQGDVYGGWNTLRPFAIDNATGELVIGTKLSASLNGNALTATKLQTPRRVSGV
                     EFDGSKDITLTAAHVAAFARRATDTYADADGGVPWNAESGAYNVTRSGDSYILVNFYT
                     GVGSCRTLQMKAHYRNGGLFYRSSRDGYGFEEDWAEVYTSKNLPPESYPVGAPIPWPS
                     DTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVLSQEQDGI
                     KSHTHSASVSSTDLGTKTTSSFDYGTKSTNNTGAHTHSLSGSTNAAGNHSHRDGRRFN
                     PSVFKDTYQYGYTSSGQNTWGVQGSVGMSTGWLANTSTDGNHSHSLSGTAASAGAHAH
                     TVGIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            22724..23305
                     /locus_tag="RHV13_002385"
     CDS             22724..23305
                     /locus_tag="RHV13_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDV1241912.1"
                     /translation="MAFRMSEQPRTIKIYNLLVGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIREAEETKNSLMQAANEHIAPLQDAVDLEIATEEE
                     NSLLEAWKKYRVLLNRVNTTTAPDIEWPVAPIG"
BASE COUNT         5720 a         5559 c         7051 g         5118 t
ORIGIN      
        1 atttccatgg cggcggcagc atgtttggta atttattgat aattaaaagt tatttctctt
       61 ttcacctgta caatattttt ttctccctgt cattagacca gtttgcaatt aattgaaata
      121 tataaataaa cctgattttc acctgccaga tggagttgct tatgtcaaat gtgagcggga
      181 tcggtgatgc ttattactgg agtgttttta aaatcgccga ggcctttggg cttcaccggg
      241 acacagtaaa aaaacggctc ctcgcggcca acactcctgt ggctgcgact gtcaggggga
      301 accccgttta cgccctgcag catgtcgggc ccgccctgtt tagtgtgaag catgaggcag
      361 cagactctgt tcatgatcca tcccgtatgg agccgaaaga gagaaaggac tggtaccagt
      421 ctgaaaatga aaggatcaag ctggaaaagg agcagcgaaa actcatccca gttgatgaag
      481 tagtcatcgt ctattcgtcc atgagaaagg ctgtcgtcca ggttctggag acaattccgg
      541 atgttcttga acgcgattgc gccctgactc ctcaggccgt cggcgttgta cagcaggcca
      601 ttgatgacct gcgatacact cttcaggaaa aatcctacga ggcttgtgct gctgaattaa
      661 ttcctgatga ggaaggagag agtctctagg aggaataatg ggtttttcat cagcccgaaa
      721 tttgggaagg gacatatcgg caggattttc cccaccacgt cgcatgccga tttcggaggc
      781 tgttaaaaaa ttcatgcgtg ttcccaaggg ggctggtaac tcggtgccat gggatcctga
      841 actgacaccc tacatcattg agcccatgaa ctgcctggca tcgcgtgaat acgatgcggt
      901 gatttttgtt ggtcctgcgc gaacagggaa gaccattggt ctgatcgatg gatggattgt
      961 ctataccatc gtttgcgatc cttcggacat gctcgttgtg cagatgaccg aagataaggc
     1021 ccgcgagcat tctaaaaagc gccttgacag aacgttcaga agcagtgcgg cggtaaagaa
     1081 aagaatgagt ccacgtcgta acgacaataa tgtccatgat aagacgttca gggatggctc
     1141 gttccttaaa attggttggc cctcggtcaa tattatgtcg tcgtcggatt accggtttgt
     1201 cgccttaacc gattacgacc gttttccgga gaatatcgac agcgagggtg atggtttctc
     1261 cctggcctca aaacgtacca ccacatttat gtccgccggg atgactctgg tggagagctc
     1321 gccgggacgt gacatctgcg acagcaaatg gcgacgtaag tcgcctcatg aagcgccacc
     1381 gacgactggt attctttccc tttacaatcg tggtgaccgc cgccgctggt actggccatg
     1441 tccgcactgc ggtgaatatt ttcagccagc tatggatgcc atgaccggct accgtaatga
     1501 accggatccc tttaaagcca gtgaggcggc gtatctactt tgcccgcact gcagcggcat
     1561 tatcactgcg gagaaaaagc gtgagctcaa tagtgcagga gtctggttgc gtgaaggtca
     1621 ggtcattgat cgtaacggca acgtttccgg tgaaccgcgc cgctcccgta tcgccagttt
     1681 ctggatggaa gggccagctg ctgcgtatca gacctgggcg caactggttt acaaattact
     1741 gactgcagaa caggagtatg aagcgacagg aagcgaagaa acactcaggg cggttatcaa
     1801 caccgactgg ggattgcctt atcttcctcg tgccagcatg gagcaacgaa aaagtgaact
     1861 gcttgagcag cgggcagagc cagttccttc ccgcagtgtt ccggatggcg ttaatttcct
     1921 tgtggcgaca gtggatgtgc aggcgggacg tcatcgccgt tttgtggttc aggtaacggg
     1981 ctatggcagc cgtggcgaac gctggattat tgatcgttac aacatcacgc agtcattgcg
     2041 cagtgactgc gacggggaga gccagcgaat tgatccggcc agctatccgg aagactggga
     2101 tgtcctgctg acggatgttt ttcataaaag ctggccgctg gcctccgatc cttctcaaca
     2161 aatgcgactg atggcaatgg cggtggactc cggcggtgaa gacggggtca ctgataatgc
     2221 ctataaattc tggcgtcgtt gccgtcgtga tggccttggt aaacgtattt acctgtttaa
     2281 gggcgacagc atccggcgcg caaaactgat cagccgtaca ttccctgata acaccggacg
     2341 aacgggccgc cgggcgcagg ccgcaggtga tgtgccgctc tggcttcttc agacggatgc
     2401 cctgaaagac cgggtgaata acgcgttatg gcgtgactcg ccaggtccag gctatgtgca
     2461 tttccctgac tggctgggga gctggtttta cgacgaactg acgtatgaag agcggagcag
     2521 tgacgggaaa tggagtaagc cgggtcgcgg tgccaacgaa gcttttgacc tgatggtgta
     2581 tgccgaggct ctggtcattc tgcatggata cgaaaagatc cgctggccgg atgcaccgga
     2641 gtgggcgagc cgggaaacct ggctggagtg tgtcccggac agtaccgaac cgtcaccctc
     2701 accggaaccg gtacccacgc ctgttaaaaa acaaaaacgg aagaaaacag taactgacga
     2761 tgttaacccc tggctgactt ccggaggatg gttatgaatc agaatgatat tgaagccatg
     2821 attcagcgtt atacggaagc tgaaatggcg gtgctggacg gaaaatccgt cacttttaat
     2881 ggtcagcaga tgaccatgga aaacttatct gagatccggc aggggcggca ggagtgggag
     2941 cgccgccttg cggctctgat tacacgacga cgggggcatc ccgggtaccg gctggcgagg
     3001 ttctgatggc aattcttgat gatgtgattg gcgttttttc accaagatgg aaagcggcaa
     3061 ggctgcgttc ccgtgcggtg atccaggctt atgaggccgt aaaaacgacg cggacacata
     3121 aagcccgacg ggagagccga actgccgacc agttaagcca gtacggggcc gtgtcgttac
     3181 gtgagcaggc ccgttacctt gataacaacc acgatctggt cattggtgta tttgacaagc
     3241 tggaagaacg ggtggtgggg aaaaacggga ttattgtcga gccacatccg gtattacgca
     3301 atggggccat tgcccgtgat ctggcagcgg agattcgcac ccgatggagt gaatggtctg
     3361 tcagtccgga ggtcaccggg cagtttaccc gtccgatgct ggaacgtctg atgctgcgta
     3421 cctggctgcg cgatggtgag gtgtttgccc agatggtttc cgggcgcata aacagcttga
     3481 cgccttctgc cggtgttcat ttctggctgg aggcgctcga gccggacttt attcccatga
     3541 ccagtgatga gagcaacagg ctgaatcagg gcgtgtttgt tgatgactgg gggcgtcccg
     3601 aaaaatatct ggtgtataaa agccgtcccg tatccgggcg gcagatggaa accaaagaag
     3661 tggatgcaga gcgaatgctg catcttaaat ttgttcgccg tctgcaccag atgcgcggga
     3721 cgtctttgtt gtccggtgtg ctgatccgcc tcagtgctct gaaagagtat gaagattctg
     3781 agctgactgc agcaaggatc gccgctgctc tggggatgta catccggaaa ggcgacggac
     3841 agagctatga aacggatggt aatgacagca aggagaatga acgcgagctt accattcagc
     3901 caggcattat ttacgacgat ctgaaacccg gcgaagaaat cggaatggtg aagtcggatc
     3961 gccccaatcc taaccttgaa acttttcgta atggtcagtt gcgtgccgtg gctgcgggca
     4021 gtcgtctgag tttttccagt acagcgcgca actataacgg cacttacagc gcccagcgtc
     4081 aggagctggt tgaatctact gatggctacc tgatcctgca ggactggttt attggtgccg
     4141 tcacccgccc gatgtaccgt gcctggctga aacaggctgt ggcatccggt gttatcaggc
     4201 taccccgcga tctggaccga tcttcactgt ataccgcggt gtattccgga ccggtgatgc
     4261 cgtggattga ccctgttaag gaggctgagg cctggaaaat tcagattcgt ggtggagcgg
     4321 cgacagaatc agactgggta cgtgcaggtg gtcgtaatcc ggatgatgtc aaacgtcggc
     4381 gcaaggccga aattgatgaa aaccgcaagc tggatctggt atttgatacc gatccggcca
     4441 gtgataaagg aggcagcagt gccgcaacga aacgacagga gccgcagcac accgacgacc
     4501 agtccgaaga ataattcctg gttcaggatg caggcaggtc accagagtga cgcggatatt
     4561 tatatttatg acgagattgg tttctggggt gttacagcga agcagtttat cagtgatctg
     4621 aatgcactgg gcgatatcac ccacattaat ctccatatta attcaccggg tggcgatgtc
     4681 tttgaaggca tcgccatttt taatgcactg aaaacacatg gtgcgtccat taccgtttat
     4741 gtcgacggtg tggcggcgtc aatggcgtcg gtcattgcga tggtgggaaa cccgatcatt
     4801 atgccggaaa actccttcat gatgattcat aaaccatttg gctttacggg cggtgatgcg
     4861 gaggacatgc gcacctatgc cgacctgctc gataaagttg aggcggttct gttacccgct
     4921 tatgcacaga aaaccgggaa aaccaccgat gaaattgctg ccatgctggc ggatgagacc
     4981 tggatgtccg gtgccgaatg tctggcacag ggatttgctg atcaggtgac accagccgtt
     5041 aaggcaatgg catgtattca gtcaaaacgt acagaggaat ttaaaaagat gccggaatcc
     5101 attcgaaaca tgattactcc gccacgcaac agtgctccac gcgtacagga tgatgaacct
     5161 gcagcctccc ggacgccagt gcaggcagca gcacccgtgg tggatgaaaa cagtatccgt
     5221 gcgcaggtac tggcagagca aaaagcgcgt gtaaacggta ttaatgatct gtttgccatg
     5281 tttggcgggc gttatcagat gctgcaggcc cagtgtcttg ccgatcctga atgttcgctg
     5341 gagcaggccc gtgaaaagct gttgaacgag atggggcgcg agtccacgcc atccaataaa
     5401 aataccccgg ctcatattta tgccggtaac ggtaattttg tgggggacgg gatccgccag
     5461 gcgctgatgg cgcgtgccgg atttgaaaaa accgaacgtg ataatgtcta caacgggatg
     5521 accctgcgtg aatatgcccg tatgtcacta actgaacggg gtattggggt ttccggttat
     5581 aacccgatgc agatggtcgg tgcggcgttc acacacagta cgtctgactt cggtaatatt
     5641 ctgctggatg ttgcgaacaa agccattctg cagggctggg aagatgcccc tgaaacctat
     5701 gaacagtgga cgcggaaagg tcagttgtct gattttaaaa ttgcccatcg tgtgggtatg
     5761 gggggcttca gtgctctgcg tcaggtgcgt gaaggggcgg aatataaata cgtcaccacc
     5821 ggagataaac aggccactat tgcactggcg acctatggcg agctgttcag tatcacccgt
     5881 caggccatta tcaatgatga tctgaatatg ctgaccgatg tcccgatgaa actgggccgt
     5941 gcggcgaaat ccactattgc cgatctggtt tatgccattc tgacgtctaa cccgaaaatc
     6001 tccacagata atgtaagtct gttcgataaa gcgaaacatg caaacgtact ggagagcgct
     6061 gcaatggacg tggcatcgct ggataaagcc cgccagttga tgcgcgttca gaaagagggg
     6121 gagcgtcatc tgaatattcg tcctgcgttc gtactggtac cgacggcgat ggagtctgtt
     6181 gctaaccagg tcattcgctc ctcaagtgtc aagggggctg acattaacgc cggtattatt
     6241 aacccggtga aagattttgc gaccgttatt gcagagcctc gtcttgatga taacagccag
     6301 accaccttct acctggctgc gtcaaaaggc tccgatacga ttgaagtggc ttatctcaac
     6361 ggtgtggata cgccatatat tgatcagatg gagggcttca gtgtggatgg cgtgacaacg
     6421 aaagtgcgta ttgacgccgg tgtcgcgcca gttgatcacc gcggtctggt gaaatgtacg
     6481 gcgtaaacgt cgcagacaac aactctgatg gcccgtaagg gctttttttg tacctgaaat
     6541 cagcccctga acggggctgt gcggagacag ttatggcaaa gaattttgta gaagaaggaa
     6601 aaacggtggc gattgttgcc agtgcagcca tcagcagcgg agatctggtg caggtgggcg
     6661 atgtttttgc ggtggcgctg accgatattc cacagggtga aacaggcgac ggcatgaccg
     6721 aaggtgtgtt tatgctgcct aagctgaaaa cggatgacat gaaaacgggt aagaaggttt
     6781 atctgaagtc cggaaaagtt cagctgacta acagcggctc tgatccgctg gtcggggttg
     6841 tctgggcaga tgccggaacc agtgcagaag aagtgccggt aaaactcaat gtctgatccc
     6901 ttttcccggc tggcagcgcg tatggatgct atcacggtta gaaagatggg aaagacagcc
     6961 tcgattaatg atgtcgatat gactgtgatc ccgggagaaa cactggcaga gctgaatgct
     7021 ctgtccggac ctgcggtctc tctggtggtg ttttcttcgg gataccgccc acggcgcggg
     7081 gatcgcgttg tttatgacgg acaacaatgg acggtcacac ggcatgaacg ttttaacggt
     7141 aagccaatga tctttattga gtaaagaggt gtgggatgaa ggggcttgag aatgccatcc
     7201 gtaatctgaa cagccttgat acccgtatgg tgccacaggc cagcgcatgg gcgataaacc
     7261 gtgtggcaca gaaagcggtc tcggtcgcca cccggcaggt tgccgggaat accgttgcgg
     7321 gagataacca ggtgaaaggg atccccctga aactggtacg tcagcgtgtc cgggtgttta
     7381 aagccagtcc gtcaggaaaa atgacggcca ggatccgcgt taaccggggc aatctgcccg
     7441 ctattaagct ggggacagcc cgggtcagac tggcccggcg tggtggaaaa ctgcagtacc
     7501 gtggcagtgt gctgaaggtg ggtaaatatc ttttccggga tgcgtttatt cagcaactgg
     7561 cgaatggtcg ctggcatgtg atgcggcgta ttgatggcaa aaatcgttac cccattgatg
     7621 tggtgaaagt cccgctgtcc ggaccgctga cacaggcatt tgaagatgcc cgcgaccgca
     7681 ttattgctgc ggaaatgccg aaacagctgg ggtatgcact gaaacaacaa ctgaggttat
     7741 ggctgacccg atgaaccgac atacacaaat ccgccaggcc gtactggcac gccttcggga
     7801 acagtgtgga gacagcgcca cgttttttga cgggcttccg gcatttattg atgcgcagga
     7861 actgcctgcc gtggcggtgt ggctgagtga tgctcagtac accggaaaaa tgacggatga
     7921 agatgactgg caggctgttc tgcatattgc tgtcttcatc cgggcacagg caccggattc
     7981 agagctggat atgtggatgg agagcaccat tttcccggcc ctgaatgata taccggcact
     8041 ttccggactc atcgacaccc tgatccctct cggttttaac tatcaacgtg ataatgagat
     8101 ggccacctgg gcgatggcgg aaatcacgta ccagatcacg tacacgaatt aaaggaggtg
     8161 gcaatgacca caccaaatcc actggcaaaa acgaaaggtg cgggaacgac gttctggatg
     8221 tacaccggca agggcgatgc gtttgcgaac cctttatcgg acactgactg gctgcgtctt
     8281 gcgatggtga aggatctgca acctggcgaa atgaccgctg atgcagaaga tgacacttat
     8341 ctcgatgatg aagatgcaga ctggaaaacg acaacccagg ggcagaaatc cgttggtgat
     8401 acttcggcga cgctggcctg gcgtccgggt gacagcgggc agaaaaaact ggttcagttg
     8461 ttcgactccg gtgaagtctg cgcgtttcgt atcaaatatc ccaacggcac tgttgatgtt
     8521 ttccgtggct ggctgagctc actgggtaaa accattgcct caaaagacgt gatgacccgc
     8581 acagtgaaaa tcagcggtgt ggggcgtccg tatctggcag aggaaggcac tgaaacagtg
     8641 agcgttaccg ggctgacggt ggcaccggca tctgccagtg taaaagtggg agcaaccacc
     8701 acgctgacct ttacagtaaa acctgacgga gccagtgaca aagcgatcag tgtgcattcg
     8761 acagatccac agactgccac ggtgaccctg aacgggcttg tggccacggt gaaaggcgtg
     8821 aagcagggca gtgtcagcat tgtgggcatg acttctgacg gcgattttgt ggcagtggct
     8881 gcggtggctg tcagcgccgc aggttaacag gacgatactc atcatttgcc ccggttatcc
     8941 ggggcttttt tgcaggtgga gaacatgatg tttctgaaac agggcacgtt taattatgaa
     9001 aagcagtccg tggtgctcag tgagctgtcc gggctgcaga gaattgaata tctggcgttt
     9061 gttcagcagc gaacggcaaa gtttgatgcc gaagagggag aactgccgga ggctgaacga
     9121 cagattgctt ttctgcggat ggggatggat atcaatgcct ggctggtttc ccgctcactg
     9181 tggaatgcgg aacagtctca ggatgttgag acgctttgcg catccgttat tacaacatgg
     9241 tcgtatgatg ccctgggagc gggggcggag atggttctgt cgctgagcgg tatgggagtc
     9301 attgagaatg ccggggattt ggagcatgag gtgctgacgc cggaaaagtc ctgacgcggg
     9361 aaatgcagtt tgtcatgcgg cttgcccggg agttccggcg ggcagactgg cggcggatgc
     9421 tgtcggaaat gtcggccact gagcttggtg agtggggcga ttatttccgg atgcagagct
     9481 tcagtgatgt gtggatggat gcgcagtttg cctcgctgaa ggcattgatc gtgagaatgg
     9541 tgtccggtag cagtgatgct gcggtggctg atttcagcct tttaccggaa gagaacggga
     9601 taccggagcg aacggacgaa gaactgatgc atcttgggga aggtatttcc ggaggtgtgc
     9661 gttatggacc agatagccaa cctggtcatt gatttgggga ttgatgcggc agagtttaaa
     9721 aatgaaattc cccgtatcaa aaaccttctg aatggtgcag ccagcgatgc agaacggtct
     9781 tctgcccgta tgcagcgttt tatggagcgt cagactcagg ccgcccggca gacaacgcag
     9841 gcagcttctt cggctgcaac agccgcatcc gtccatgcgc agacggtgga gaagaacgca
     9901 caggctcatg aacgcatggc ccgcgaggtg gagaaaaccc gccagcgcat ggaggcactg
     9961 agccagaaaa tgcgcgagga acaggcgcag gccatggctc tggcggaggc tcaggataaa
    10021 gcggctgctg cgttttatcg tcagattgac agtgtgaaac aggccagtgc ggggctgcag
    10081 gaattacagc gtattcagca gcagatccga caggccagaa acagtggcgg gattggtcag
    10141 caggattatc tggcgctgat ttctgaggtt actgcgaaaa ctcgtgttct tacacaggct
    10201 gaggaagagg ctacccgaca gaaagtggcg tttatccgtc agcttaaaga gcaggcaacc
    10261 cgccagaatc tttcttcttc tgagttgctt cgtgctaagg ctgcccagct gggggtaagc
    10321 agtgctgcag aagtgtatat ccgcaaaatg gagcaggcag gaaaagccac gcattcgctg
    10381 ggtctgaaaa gtgcagcggc ccgccaggag ataggcgttc tgataggtga actggctcgc
    10441 ggcaatttag gtgcgctgag gggatccggg ataacgctgg ctaaccgtgc cggatggata
    10501 gacacactga tgtcaccgaa aggcatgatg ctgggcgggg ttattggcgg tattgccgcg
    10561 gccgtctatg gtctgggtaa agcctggtat gatggtcaga aggaggggga agaatttaac
    10621 cgccagttgt cgctgacggg gcattatgcc ggagtcactg ccgggcagct gtggacgctc
    10681 agtcgtgcta tttccgggaa tggtatcacg caacatgctg cagccggtgc gctggctcag
    10741 gtggtgggga gtggtgcatt tcgtggaaac gatatcggta tggtggcgag agctgccgca
    10801 cagatggagc gatcggttgg ccagtcggtc agcgatacca taaatcagtt taagcggctg
    10861 aaggatgatc ctgtaaatgc cgcgaaggct ctggacaatg agctgcattt tcttactgcc
    10921 actcagcttg agcagatacg cgtccttggg gaacaggggc ggtccagtga tgcggcacgg
    10981 atagccatgt ctgcactggc agaggaaacc ggtcggcgta ctgcggatat tgataataac
    11041 ctcaatgcgc tgggcagtac gctgaagtat ctgtctgatt tatggagtcg tttctgggat
    11101 gcggccatga atattggtcg tgaagactcg ctggatgaac agatttccgc tttacaggag
    11161 aaagtgtcgc gggcgaaaag actcccctgg acggcatcat cttctcaggt tgagtacgat
    11221 cagcagcgtc ttaacgagct tcagaagaaa aaacgccaga aggatttgca ggatgcaaaa
    11281 gagcaggcag agcagaatta tcaggagcaa cagaaacgcc gtaatgctga aaatgctgca
    11341 ctgaaccgga tgaatgaaac ggaagcagca cgacatcagc gtgaaattgc gcgtattaat
    11401 tccatgcagt acgccgatca ggctgtcagg gatgcggcga tacaacgtga aaatgaacgt
    11461 tacgagaaag ccctggcatc cggtaagaaa aaaacacgcg aaccccgtaa tgatgaggcc
    11521 acccggttat tgctgcagta cagtcagcaa caggcacagg tggaaggaca gattgctgct
    11581 gccagacagt cagcaggcat tgccacggaa aggatgacag aagcgcataa acagcttctg
    11641 gctctgcagc agcgcatcag cgacctggac gggaaaaaac tgacggcaga tgaaaagagt
    11701 gtgctggccc gtaaagatga actgattcag gcactgacgc tgctggatgt aaaacagcag
    11761 gagcttcaga aacagacggc actcaacgag ctgaagaaaa aaacaattca gctgaccagt
    11821 caactggctg aagaagagcg cgctcagcgt cagcaacatg acctggatat cgccacggtg
    11881 ggtatgggtg atcagcagcg gcagcgatat caggtacaac tgagtcttcg ccagaaatac
    11941 cagcaacagc tggagcagtt gaggcgggat agtgagcaga aaggaacata taacacggat
    12001 gactacagaa aggccgagca ggcgctgacg gagagcctga accgacaact gaatgagaat
    12061 cgccgttact ggcaacagct tgaagttgtg cagggtaact ggaaaaacgg agtcctgcgt
    12121 gcatttcaga attttaccgt ggatgcagat aatacggcag gaacagcaga acaggtgttc
    12181 tcgtcagcct tcagcaacat gggaaatggc ctggcaactt ttgtcactac cggcaaactc
    12241 aatttcaaat ccttcacctc ttctgtgctg tcagatatgg cgaaaatcct ggcgcaggca
    12301 accatgatga aatcgataaa agggattggc agtgtactgg gatttgatct cagcagcctt
    12361 tccctgaatg ccaatggggg gatttatcag tctgctgatt tgagtcgtta cagtggcacg
    12421 gtggttaacc gtccgacgtt ttttgctttt gcaaaaggcg cgggtgtgat gggggaagcg
    12481 ggacctgaag ccattctgcc actgcgtcgt ggtgctgacg gtaagctggg ggttgtggcg
    12541 gatattggtg gttcaggtat ggcgatgttt gccccgcagt acaacatcga gatcaataac
    12601 gatggcacga acgggcagat aggtccggct gccctgaagg tggtttatga cctcgggaaa
    12661 aaagcggcag cggactttat gcaacagcag gcccgtgatg gtggtcggtt aagtggagca
    12721 tatcggtaat ggagacgttt cactggaaag tgcgcccgga tatgaatgtg gtatcagagc
    12781 cgaaagtggt gacagtgaag ctgggcgatg gttatgaaca gcgtcgtgcg gcgggactga
    12841 ataaccagtt gtcgatttac agcgtgacga tacgtgttcg taaatgtgaa cacccatctt
    12901 taaaagcctt tctggaacgg cacggtggcg tccgcgcatt tcagtggacg ccaccttatg
    12961 actggaagcc gatcagggtg gtttgtcgta aatggtcggc aagcgtgggg gcgctgtggg
    13021 taaccataac ggcagatttt gaacaggtcg tggcatagga ggccctgatg caggatattt
    13081 cacaggaaac acatcatgag acgacacgcc tcactcagtc agcccaggtg gtgctctggg
    13141 aaatcgatct gacagtggtc ggtggtgaac gttatttttt ctgtaatgag cagaacgata
    13201 aaggtgagcc ggtcacctgg caggggcgac agtatcaggc gtatcccatt caggggacgg
    13261 gatttgaact gaacggcaaa ggcagtgctg cccgtccgac actgacggtt tctaacctgc
    13321 acggcatggt caccgggatg gcggaagacc tgcagagtct ggtcggagga acggtggtcc
    13381 ggcgtaaggt ttacgcccgt tttctggatg cggtgaactt cgtcaacgga aacagtgacg
    13441 ccgatccgga gcaggaggtg atcagccgct ggcgcatcga gcagtgcagt gaactgagcg
    13501 cggtgagtgc ctcttttgta ctgtccacgc cgacggaaac ggacggtgcc gtttttccgg
    13561 gacgtatcat gctggccaac acctgcacct ggacctatcg cggtgatgag tgcggttatc
    13621 acggtccggc ggtcgcggat gaatatgatc agccgacgtc cgatatcacg aaggataaat
    13681 gcagcaaatg cctgagcggc tgtaagtttc gcaataacgt cggcaacttt ggcggcttcc
    13741 tttccattaa caaactttcg cagtgaatcc catgacagag acagaatcag cgattctggc
    13801 gcacgcccgg cgatgtgcgc cagcggagtc gtgcggcttc gtggtgagaa caccggaggg
    13861 ggaaagatat tttccctgcg tgaatatatc cggtgagccg gaggcgtatt tccggatgtc
    13921 gccggaggac tggctgagtg cagaaatgca gggagagatt gtggcgctgg tccacagcca
    13981 ccccggtggt ctgccctggc tgagtgaggc cgaccggcgg ctgcaggtgc agagtgattt
    14041 gccgtggtgg ctggtctgcc ggggggcgat tcataaattc cgctgtgtgc cgcatctcac
    14101 cgggcggcgc tttgagcacg gggtgacgga ctgttacacg ctgttccggg acgcttacca
    14161 tctggcggga attgagatgc cggattttca tcgcggggat gactggtggc gtcacggtca
    14221 gaatctctat ctggataatc tggaggccac agggctgtat caggtgccgt tgtcatcagc
    14281 acaaccgggc gatgtgctgc tgtgctgctt tggttcatcg gtgccgaatc atgccgccat
    14341 ttactgtggt gacggcgagc tgctgcacca tattcctgaa caactgagca aacgagagag
    14401 gtataccgac aaatggcagc gacgcacaca ctccctctgg cgtcaccggg catggcgcgc
    14461 atctgccttt acggggattt acaacgattt ggccgccgca tcgatctgcg tgtgaaaacg
    14521 ggggccgaag ccatccgggc gctggccatg cagatcccgg cgtttcgtca gaagctgagc
    14581 gacggctggt atcaggtacg cattgccggg cgtgatacag gtgaaacgga attatcagcc
    14641 cgtcttaatg aaccgctggc aaatggtgct gtgatccata tcgtgccgcg tctggcggga
    14701 gccaaaagtg gcggtgtgtt tcaggctgtg ctgggggcgg ctgttatggc ggttgctata
    14761 tggatgccgg gggtaggaat tatggcgagt aatctgctgt tttctctcgg tgccagtatg
    14821 acgcttggcg gtgttgcaca gatgctggcc cctaaaccca aaaccccccg cacacagaca
    14881 acggataacg gcaaacagaa cacctatttt tcttcactgg ataacatggt tgcccagggc
    14941 aatgttctgc ctgttctgta cggtgaaatg cgcgtggggt cacgcgtggt ttctcaggag
    15001 atcagcacgg cagacgaagg ggatggtggt caggttgtgg tgattggtcg ctgatgcaaa
    15061 atgttttatg tgaaaccgcc tgcgggcggt tttgtcgttt atggagcgtg aggaatgggt
    15121 aaaggaagca gtaaggggca taccccgcgc gaagcgaagg acaacctgaa gtccacgcag
    15181 ttgctgagtg tgattgatgc catcagtgaa gggccgattg aaggtccggt ggatggatta
    15241 aaaagcgtgc tgctgaacag tacgccggtg ctggacagtg atgggaatac caacatcgcc
    15301 ggtgtcacgg tggtgttccg ggctggtgag caggagcaga ctccgccgga gggatttgaa
    15361 tcctccggct ccgagacggt gttgggtacg gaagtgaaat acgacacgcc gatcacccgc
    15421 gccatcacgt cggcaaacat cgaccgactg cgctttacct tcggtgtgca ggcactggtg
    15481 gaaaccacct caaaggggga ccggaatccg tcggaagttc gcctgctggt tcagatacag
    15541 cgtaatggtg gctgggtgac ggaaaaagac atcaccatta agggcaaaac cacctcgcag
    15601 tatctggcct cggtggtggt ggataacctg ccgccgcgcc cgtttaatat ccggatgcgc
    15661 agaatgacgc cggacagcac cacagaccag ctgcagaaca aaacgctctg gtcgtcatac
    15721 accgaaatca tcgatgtgaa acagtgctac ccgaacacgg cactggtcgg cgtgcaggtg
    15781 gactcggaac agttcggcag ccagcaggtg agccgtaatt atcatcttcg cgggcgcatt
    15841 ctgcaggtgc cgtcgaacta taacccgcag acgcggcaat acagcggtat ctgggacgga
    15901 acgtttaagc cagcatacag caacaacatg gcctggtgtc tgtgggatat gctgacccat
    15961 ccgcgctacg gcatggggaa gcgtctcggt gcggcggatg tggacaaatg ggcgctgtat
    16021 gtcatcggcc agaattgcga ccagtcggtg ccggatggct ttggtggcac ggagccgcgc
    16081 atcacctgta atgcctggct gaccacacag cgcaaggcgt gggatgttct cagtgatttc
    16141 tgctcggcga tgcgctgtat gccggtatgg aacgggcaga cgctgacgtt cgtgcaggac
    16201 cgaccgtcgg ataaggtgtg gacctataac cgcagtaatg tggtgatgcc ggatgatggc
    16261 gcgccgttcc gctacagctt cagtgccctg aaggaccgtc ataatgccgt tgaggtgaac
    16321 tggattgacc cggataacgg ctgggaggcg gcgacagagc ttgtggagga cacgcaggcc
    16381 attgcccgtt acggtcgtaa cgtcacgaag atggatgcct ttggctgtac cagccggggg
    16441 caggcacacc gcgccgggct gtggctgatt aaaacggaac tgctggaaac gcagaccgtg
    16501 gacttcagcg tgggtgccga agggcttcgc catgtaccag gtgatgtcat tgaaatctgt
    16561 gatgatgact atgcggggat cagcaccggc gggcgcgtgc tggcggtgaa cagccagacc
    16621 cggacgctga cgctcgaccg tgaaatcacg ctgccatcct ccggtaccac gctgataagc
    16681 ctggttgacg gaagtggcaa tccggtcagc gtggaggtcc agtccgtcac cgacggcgtg
    16741 aaggtaaaag tgagccgtgt tcctgacggc gttgccggat acagcgtgtg ggggctgaag
    16801 ctgccgacgc tgcgccagcg cctgttccgc tgcgtgagta tccgtgagaa cgacgacggc
    16861 acgtatgcca tcaccgccgt gcagcatgta ccggaaaaag aagccatcgt ggataacggg
    16921 gcgcactttg acggcgacca gagcggcacg gtgaatggtg tcacgccgcc agcagtgcag
    16981 cacctgactg ccgaagtcac cgcagacagc ggggaatatc aggtgctggc gcgctgggac
    17041 acgccgaagg tggtgaaggg ggtgagcttt atgcttcgcc tgaccgtaac agcggacgac
    17101 ggcagtgagc ggctggtcag cacggcccgg acgacagaaa ccacataccg cttcaggcag
    17161 ctggcgctgg ggcgttacac gctgacggtc cgggcggtaa atgcgtgggg acagcagggc
    17221 gatccggcat cggtatcgtt ccggattgcc gcaccggcag cgccgtcgca gattgagctg
    17281 acacccgggt attttcagat aaccgccacg ccgcatcttg cggtttatga tccgacggta
    17341 cagtttgagt tctggttctc ggaaaagcgg attgcggata tcaggcaggt tgaaaccagc
    17401 gcgcgttatc ttggtacggc gctgtactgg atagccgcca gtatcaatat caggccgggc
    17461 catgattatt atttttacgt tcgcagtgtg aataccgttg gcaaatcggc attcgtggag
    17521 gctgttggtc ggccgagtga tgatgcttcc ggctatctgg attttttcaa aggcgagata
    17581 gggaaaagcc atctggctca ggagttgtgg acgcagattg ataacggtca gcttgcgcct
    17641 gacctggctg aaatcaggac gtccattacg gatgtcagca atgaaatcac acagaccgtc
    17701 aataagaaac tggaagatca gagtgcagcg atccagcaga tacagaaggt tcaggttgat
    17761 acaaataata acctgaacag catgtgggct gtgaagctgc agcagatgca ggacggacgc
    17821 ctttatatcg cgggtatcgg tgccggtatt gagaatacgc cagcaggaat gcagagtcag
    17881 gtgctgctgg cggcagacag gattgcgatg attaatcctg caaatggcaa cacaaaaccg
    17941 atgtttgttg gtcagggtga tcagatattc atgaatgaag tgttcctgaa atatctgacg
    18001 gctcccacca ttaccagcgg cggtaatcct cctgcatttt ccctgacacc ggacggaaag
    18061 ctgaccgcta aaaatgcaga tatcagtggc agtgtgaatg cgaactccgg gacgctcaac
    18121 aacgttacga taaatgagaa ctgtcagatt aaagggaaac tgtcagccaa ccagattgaa
    18181 ggcgatattg tcaaaacagt gggtaaggct ttcccgcggg actcccgggc accggagcgg
    18241 tggccatcag ggaccattac cgtcaggatt tatgacgatc agccgtttga ccggcagatt
    18301 gttattccgg cggtggcatt cagcggtgct aaacatgaga gagagcatac tgatatttac
    18361 tcctcatgcc gcctgatagt gaagaaaaat ggtgctgaaa tttataaccg taccgcgctg
    18421 gataatacgc tgatttacag tggtgttatt gatatgccag ctggtcacgg ccacatgacg
    18481 ctggagtttt cggtgtcagc atggctggta aatgactggt atcccacagc aagtatcagc
    18541 gatttgctgg ttgtggtgat gaagaaagcc accgcaggca tcagtatcag ctgaatttta
    18601 taacccatat acgggcgcca gaaatggcgc cttttttatt gcagaaaagc gagaggtaat
    18661 tatgcgtaaa gtttgtgcag ccattttgtc cgcagccatc tgtctggccg tatccggtgc
    18721 gcctgcatgg gcgtctgagc agcaggccac actgagcgca gggtatcttc atgcccgtac
    18781 gaacgctccc ggcagcgata atctgaacgg gattaacgtg aaataccgtt atgagtttac
    18841 ggacacgctg gggctgatta cgtcattcag ttatgccaac gctgaagatg agcaaaaaac
    18901 gcattacagc gatacccgct ggcatgagga ttccgtgcgt aatcgctggt tcagcgtgat
    18961 ggcggggccg tctgtgcgcg tgaatgaatg gttcagcgcg tatgcgatgg cgggtatggc
    19021 ttacagccgt gtgtcgactt tctccgggga ttatatccgc gtaactgaca acaaggggaa
    19081 aacgcacgat gtgctgaccg gaagtgatga cggtcgccac agcaacacgt ctctggcgtg
    19141 gggagctggc gtgcagttta acccgaccga atccgtggcc gttgacgtcg cttatgaagg
    19201 ctccggcagc ggtgactggc gtaccaacag tttcatcgtt ggtgtcggtt ataaattctg
    19261 attagccagg taacacagtg ttatgacagc ccgccggttc aggcgggctt ttttgtgggg
    19321 ggaatatggc agtacggatt tcaggtgtac tgaaagatgg cgcaggtaag ccgatacaaa
    19381 actgcaccat tcagctaaag gccaggcgca acagcaccac ggtggtggtg aacacagtgg
    19441 cctcagaaaa cccggatgaa gccgggcgtt acacaatgga cgtcgagtat ggtcagtaca
    19501 gcgtcagtct gttggtggag ggattcccgc cgtcacacgc cgggattatc accgtatatg
    19561 aggactcaaa gccgggcaca ctgaatgatt tcctcggtgc aatgacggag gatgatgtcc
    19621 ggccagaggc actgcgccgt tttgaactga tggtggaaga ggtggcgcgt aacgcgtccg
    19681 cagtggcaca gaacacggca gccgcgaaga agtcagccgg cgatgccggc acatctgccc
    19741 gtgaggcggc aacccatgcg actgatgctg caggctcagc acgcgcagcc agcacgtcag
    19801 ccggacaggc cgcttcgtcg gctcagtcag cgtcttccag cgcaggaacg gcatcaacaa
    19861 aggccactga agcatcgaaa agtgctgctg ctgcagagtc ctcaaaaagc gcggcagcta
    19921 ccagtgccag tgccgcgaaa acgtcagaaa cgaatgcggc agcgtcacaa caatcagcag
    19981 ccacttctgc atccaccgcg accacgaaag cgtcagaagc agccacctca gcccgggatg
    20041 cgtcagcttc aaaagaggcg gcaaaatcat cagaaacgag cgcagcctcg agcgccagca
    20101 gcgcagcttc ctcggcaacg gcgacagcaa attccgcgaa ggcggcaaaa acgtcagaga
    20161 cgaacgccag gtcttctgaa acggcagcgg gacagagcgc ctcggctgcg gcaggctcaa
    20221 aaacagcggc tgcgtcgtct gccagtgcag cgtcaacaag tgccgggcag gcctcagcca
    20281 gtgccaccgc cgccggaaaa tcggcagaaa gcgccgcatc gtctgcttca acagccacaa
    20341 cgaaggctgg cgaagccact gaacaggcca gcgcagcagc gaggtctgct tccgcagcga
    20401 agacatccga aacgaacgcg aaagcgtcgg aaaccagcgc agaatcctca aaaacggctg
    20461 ccgcatcgtc cgccagttcg gcggcgtcat cggcatcatc tgcgtctgct tcaaaagatg
    20521 aggcgaccag acaggcgtca gcagcgaaga gcagcgccac gacggcatcc acgaaggcga
    20581 cagaggcagc tggcagtgcg acggcggcag ctcagagcaa aagtacggcg gaatccgcgg
    20641 caacgcgcgc cgagacagca gctaaacggg cagaggatat tgcatccgcc gtggcgcttg
    20701 aggatgcaag tacgacgaaa aaggggatag tacagctcag cagcgcgacc aacagcacgt
    20761 ctgaaacgct ggcggcaacg ccaaaggcag taaaagcagc ctatgacaat gcagagaaac
    20821 gtctgcagaa agaccagaac ggcgctgata tacccgataa gggacgcttc ctgaacaaca
    20881 ttaacgcggt cagtaaaaca gactttgctg ataagcgtgg tatgcgttat gtgcgggtta
    20941 acgctcctgc aggtgcaaca tctggaaaat attatcctgt tgttgttatg cgttctgctg
    21001 gctcagtaag cgaactggca tcaagggtca ttatcaccac ggcaacgcga accgcaggcg
    21061 atccgatgaa taactgcgag tttaacggat ttgttatgcc tggtggctgg actgacaggg
    21121 ggcgttatgc ttatggaatg ttctggcaat atcaaaacaa tgaacgagcc atccactcaa
    21181 taatgatgag taataagggc gatgatttgc gctctgtgtt ctatgttgat ggcgctgctt
    21241 tccctgtttt tgcgtttatc gaagatggcc tgtcaatatc cgcacctggt gctgatctcg
    21301 ttgttaatga tacgacctat aagtttgggg caacaaatcc ggcgactgaa tgtatcgcgg
    21361 cggacgttat ccttgatttt aagagtgggc gtggttttta tgagtcccat tcgttaatcg
    21421 ttaacgataa cttgtcgtgc aaaaaacttt ttgccacaga cgaaattgta gcgcgtggtg
    21481 gtaatcagat tcgaatgata ggtggggagt atggggcatt atggcgtaat gatggcgcta
    21541 aaacttacct gctgcttacc aatcaaggtg atgtttatgg tggatggaat acattaagac
    21601 cgtttgctat tgataacgca accggcgaac tggttattgg aaccaagctg tctgcaagtc
    21661 tgaacggtaa cgcattgaca gcaacaaagc tgcaaacgcc aagacgggtt tctggtgttg
    21721 agtttgatgg ttccaaagat attactttaa ccgccgcgca tgtggctgct tttgccagaa
    21781 gggcaacgga tacatatgcc gatgcggatg gtggcgttcc ctggaatgcc gaatcaggcg
    21841 cttataatgt cacccgctct ggcgacagct atattctggt taacttctat accggagtcg
    21901 gaagttgccg gaccttgcag atgaaggcgc attacagaaa tggtggtctg ttctaccgtt
    21961 cttcaagaga cggttatggt tttgaggaag actgggcaga agtttatacc tcgaaaaatc
    22021 ttccaccaga aagctaccca gtcggcgcac caatcccgtg gccatcagat accgttccgt
    22081 ctggttatgc cctgatgcag gggcagactt ttgacaaatc tgcttacccg aaacttgcgg
    22141 ccgcttatcc gtcaggcgtg atccctgata tgcgtggctg gacgattaag ggcaagcccg
    22201 ccagtggtcg tgccgtattg tctcaggaac aggacggcat taaatcgcac acccacagcg
    22261 ccagcgtatc cagtacggat ttgggtacga aaaccacatc atcgtttgat tacggcacta
    22321 aatccacgaa taacactggt gcgcatactc atagtttaag tggcagcacg aatgcagctg
    22381 gtaatcacag ccatagagat ggccgtcgat ttaaccccag tgtttttaaa gatacttatc
    22441 aatatggtta tacaagctca ggtcaaaata cctggggtgt acaaggttca gtaggtatgt
    22501 ctacggggtg gttagctaat accagtacag atggtaatca tagccactca ctgtccggca
    22561 cagcagcatc tgcaggtgca cacgcgcata ctgtcggtat tggtgctcat acgcactccg
    22621 ttgcgattgg ttcacatgga cacaccatca ccgttaacgc tgctggtaat gcggaaaaca
    22681 ccgtcaaaaa catcgcattt aactatattg tgaggcttgc ataatggcat tcagaatgag
    22741 tgaacaacca cggaccataa aaatttataa tctgctggtc ggaactaatg aatttattgg
    22801 tgaaggtgac gcatatattc cgcctcatac aggtctgcca gcaaacagta ccgatattgc
    22861 accaccagat attcctgctg gctttgtggc tgttttcaac agtgatgagg catcgtggca
    22921 tctcgttgaa gaccatcggg gtaaaacggt ttatgacgtg gcttccggcg acgcgttatt
    22981 tatttctgaa ctcggctcat taccggaaaa tgtcacctgg ttatccccgg aaggggagtt
    23041 tcagaagtgg aacggcacag cctgggtgaa agatgcagaa gcagaaaaac tattccggat
    23101 ccgggaggcg gaagaaacaa aaaacagcct gatgcaggca gcgaatgagc atattgcgcc
    23161 acttcaggat gctgtagatc tggaaatcgc aacggaggaa gaaaactcgt tgctggaagc
    23221 ctggaaaaag tatcgggtgt tgctgaaccg tgttaataca acaactgcac cggatattga
    23281 atggccagta gcacctatag ggtaaattcg taatgattac ctaaatacgt tattcttttg
    23341 ttcaaaaagt gattttgcta taggtaggga gtatgttctt ggattaagaa taaaaaatcg
    23401 ccatgaaaga gcttcatggc gagtacataa acgtacaaaa tgtgatga
//
LOCUS       DAPGOF010000071        23198 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.071, whole genome shotgun sequence.
ACCESSION   DAPGOF010000071 DAPGOF010000000
VERSION     DAPGOF010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.071"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1054
                     /locus_tag="RHV13_002386"
     CDS             <1..1054
                     /locus_tag="RHV13_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDV1241913.1"
                     /translation="IHKPINGSTLIQTKSNDVSIFYIFRQLSFICLTSLALFCYGKDN
                     ILDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNHTATWGINYTK
                     ILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYISRGI
                     EVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDFC
                     VMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDILARLEGEVFGLLFTELN
                     SAQAKIIAERMRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTRNISLVMTEADKAL
                     REAKSEGGNKVIIHHI"
     gene            1235..2725
                     /locus_tag="RHV13_002387"
     CDS             1235..2725
                     /locus_tag="RHV13_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDV1241914.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYLDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKLINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIASQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            2768..3271
                     /gene="yeaK"
                     /locus_tag="RHV13_002388"
     CDS             2768..3271
                     /gene="yeaK"
                     /locus_tag="RHV13_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HDV1241915.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGADFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     INFRRTA"
     gene            complement(3272..3376)
                     /locus_tag="RHV13_002389"
     CDS             complement(3272..3376)
                     /locus_tag="RHV13_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1241916.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            3546..3992
                     /locus_tag="RHV13_002390"
     CDS             3546..3992
                     /locus_tag="RHV13_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HDV1241917.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(3949..4770)
                     /gene="nimR"
                     /locus_tag="RHV13_002391"
     CDS             complement(3949..4770)
                     /gene="nimR"
                     /locus_tag="RHV13_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HDV1241918.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RAMVEMMAKEPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIVALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSLQSAKPDPRQ"
     gene            4867..6048
                     /gene="nimT"
                     /locus_tag="RHV13_002392"
     CDS             4867..6048
                     /gene="nimT"
                     /locus_tag="RHV13_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HDV1241919.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLIYYVIIGWLPAILISHGYNEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6103..6450
                     /locus_tag="RHV13_002393"
     CDS             6103..6450
                     /locus_tag="RHV13_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HDV1241920.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGDVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6472..6726)
                     /gene="yoaF"
                     /locus_tag="RHV13_002394"
     CDS             complement(6472..6726)
                     /gene="yoaF"
                     /locus_tag="RHV13_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HDV1241921.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            6909..7934
                     /gene="dgcP"
                     /locus_tag="RHV13_002395"
     CDS             6909..7934
                     /gene="dgcP"
                     /locus_tag="RHV13_002395"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HDV1241922.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            7967..8065
                     /locus_tag="RHV13_002396"
     CDS             7967..8065
                     /locus_tag="RHV13_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HDV1241923.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8068..8142
                     /gene="yoaJ"
                     /locus_tag="RHV13_002397"
     CDS             8068..8142
                     /gene="yoaJ"
                     /locus_tag="RHV13_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HDV1241924.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8201..8449)
                     /locus_tag="RHV13_002398"
     CDS             complement(8201..8449)
                     /locus_tag="RHV13_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDV1241925.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(8597..8779)
                     /locus_tag="RHV13_002399"
     CDS             complement(8597..8779)
                     /locus_tag="RHV13_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HDV1241926.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(8783..9142)
                     /gene="yeaR"
                     /locus_tag="RHV13_002400"
     CDS             complement(8783..9142)
                     /gene="yeaR"
                     /locus_tag="RHV13_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HDV1241927.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(9315..9953)
                     /gene="leuE"
                     /locus_tag="RHV13_002401"
     CDS             complement(9315..9953)
                     /gene="leuE"
                     /locus_tag="RHV13_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HDV1241928.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NSEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(10080..11003)
                     /gene="dmlR"
                     /locus_tag="RHV13_002402"
     CDS             complement(10080..11003)
                     /gene="dmlR"
                     /locus_tag="RHV13_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HDV1241929.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            11106..12191
                     /gene="dmlA"
                     /locus_tag="RHV13_002403"
     CDS             11106..12191
                     /gene="dmlA"
                     /locus_tag="RHV13_002403"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HDV1241930.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            12442..14052
                     /gene="yeaV"
                     /locus_tag="RHV13_002404"
     CDS             12442..14052
                     /gene="yeaV"
                     /locus_tag="RHV13_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HDV1241931.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            14084..15208
                     /gene="yeaW"
                     /locus_tag="RHV13_002405"
     CDS             14084..15208
                     /gene="yeaW"
                     /locus_tag="RHV13_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
                     /protein_id="HDV1241932.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEVLTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            15264..16229
                     /gene="yeaX"
                     /locus_tag="RHV13_002406"
     CDS             15264..16229
                     /gene="yeaX"
                     /locus_tag="RHV13_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HDV1241933.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(16283..17398)
                     /gene="rnd"
                     /locus_tag="RHV13_002407"
     CDS             complement(16283..17398)
                     /gene="rnd"
                     /locus_tag="RHV13_002407"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HDV1241934.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(17480..19165)
                     /gene="fadD"
                     /locus_tag="RHV13_002408"
     CDS             complement(17480..19165)
                     /gene="fadD"
                     /locus_tag="RHV13_002408"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HDV1241935.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(19370..19951)
                     /gene="yeaY"
                     /locus_tag="RHV13_002409"
     CDS             complement(19370..19951)
                     /gene="yeaY"
                     /locus_tag="RHV13_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HDV1241936.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(19991..20686)
                     /gene="tsaB"
                     /locus_tag="RHV13_002410"
     CDS             complement(19991..20686)
                     /gene="tsaB"
                     /locus_tag="RHV13_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDV1241937.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(20744..22654)
                     /locus_tag="RHV13_002411"
     CDS             complement(20744..22654)
                     /locus_tag="RHV13_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HDV1241938.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCNDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            22786..23130
                     /locus_tag="RHV13_002412"
     CDS             22786..23130
                     /locus_tag="RHV13_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDV1241939.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         5664 a         5917 c         5660 g         5957 t
ORIGIN      
        1 tatccataaa ccaattaacg gcagtacact gatccagaca aagtcgaatg atgtttctat
       61 tttctatatt ttccgccaac tcagttttat ttgtttaacc tcgctggcgc tcttttgtta
      121 tggaaaagac aacatccttg acaacaataa gaaaaaaact ggcattctgt tgctggcgct
      181 gattcctttt ttagtttttc cccttctggc acacaatctg agcagttata acgctgacta
      241 ttctttgtat gtcgtcgatt actgtccgga caaccatact gcgacctggg gaatcaacta
      301 tacaaaaata ttggtttgtc tgtgggcatt tttactgttc tttattatca tgcgtacacg
      361 attagccagc gaactatggc cgttaatagc attattatgt ctggcatcgc tatgctgcaa
      421 cttacttcta ctgactctgg atgagtataa ttataccatc tggtatatca gtcgcgggat
      481 tgaagtctcc agtaaactgt ttgttgtgtc ttttctgatt tataacattt ttcaggaact
      541 gcaactctcc agcaaactgg cagttcatga tgtgctgacc aatatttata atcggcgcta
      601 ctttttcaac agcgtagagt cattattgtc gcgacctgtt gttaaggact tctgtgtcat
      661 gctggttgat attaatcagt tcaaacgcat caatgcccaa tggggacatc gtgtgggtga
      721 taaagtgctg gtttcaattg tcgatattat ccagcaaagc atccgccccg atgatatttt
      781 agcgcgactg gagggtgagg tgtttggctt gctatttacc gaactcaata gtgcccaggc
      841 aaaaatcatt gcggaacgta tgcgtaaaaa tgtcgaactc ctgaccggct ttagtaacag
      901 atatgatgtt cctgaacaaa tgaccatcag tattggcacg gttttttcaa cgggtgacac
      961 gcgtaatatc tcgcttgtca tgacggaagc agataaagcc ttacgcgaag cgaaaagcga
     1021 ggggggcaac aaagtgatta ttcatcatat ttaagtgcaa aaatattcag agccatgctt
     1081 tttgcgtggc ttttgcatac aatttattac tattacccct aaatttcccc tccccagcgg
     1141 cgcagagatg agtataatta gcgcccctgt gccaggccgc aatcgaactt tatctggttt
     1201 tctcgtttca ctaaccgaag gagtgccatt tatcatgaaa ttgcaccata gaatgctccg
     1261 gcattttatc gccgcaagtg tcattgtgct gacatcttcc ttccttattt ttgaacttgt
     1321 cgccagcgac agagcaatga gtgcctatct gcgctatatc gtgcagaaag cagactcctc
     1381 ctttctttat gataagtatc agaatcagag tattgccgcg catgtgatgc gcgctctcgc
     1441 tgctgagcag tcggaagtgt cgccagaaca gcggcgcgcc atctgcgagg cttttgagtc
     1501 tgccaataat acccatggct taaacctgac tgcccataaa tacccgggct tacgcggcac
     1561 actacaaacc gcatccactg actgcgacac aattgtggaa gctgcagcac tattacccgc
     1621 ttttgatcag gcagtggaag gcaaccgcca ccaggatgat tacggttcag gtcttgggat
     1681 ggccgaagag aaatttcact attatctcga tctcaatgac cgctatgtct atttttatga
     1741 gccggttaat gttgaatact ttgcgatgaa taactggtcc ttcctgcagt caggaagtat
     1801 tggcatcgat cgcaaagata ttgaaaaggt atttaccggg cgtaccgtat tatcgagcat
     1861 ttacctggat cagcgtacta aacagaacgt gatgagtttg ctgacgccgg tatatgtcgc
     1921 agggcagcta aaagggattg tgctgctgga tattaacaaa aacaatctgc ggaatatctt
     1981 ttacactcat gaccgccctc tcctctggcg ttttctcaat gtcacgctaa ccgatacaga
     2041 ttcggggcgc gacattatca tcaaccagag cgaagataat ctgttccagt atgtcagtta
     2101 cgtccatgac ttaccgggcg gcattcgtgt ctcgttatcc attgatattc tttactttat
     2161 cacgtcttcg tggaaaagcg ttctgttctg gattttgacg gcgttaattt tgctgaatat
     2221 ggtgcggatg cacttccgct tataccaaaa tgtgtcgcga gaaaatatta gtgatgcgat
     2281 gactggactg tataatcgca aaattttaac ccctgaactg gagcagcggt tgcagaaact
     2341 ggtgcaatcc ggttcttcgg tgatgtttat tgcaattgac atggacaagt taaagctaat
     2401 aaatgacacc ctcggtcatc aggaagggga tttagcgatt acgttgttag ctcaggcgat
     2461 taaacaatcg attcgtaaaa gtgattatgc catccgactc ggtggcgatg aattctgcat
     2521 cattcttgtc gattcgacgc cgcaaattgc atcacaactg cctgaacgta tcgaaaaacg
     2581 tctgcaacat atcgcgccgc agaaagagat cggcttctct tccggtattt acgcgatgaa
     2641 agaaaacgat acgttacatg atgcgtataa agcttccgat gagcgtttat atgtcaataa
     2701 gcagaacaaa aacagccgtt catgataacc ttctgtggtt gtttgcttgt aatctcagga
     2761 gcatgaaatg actgaaatgg ctaaaggaag cgtgacgcat cagcggttaa tcgctttatt
     2821 atcacaagaa ggtgcagact tccgcgtcgt tacccatgaa gcggtaggta aatgtgaagc
     2881 ggtgtcagaa attcgtggca ccgctttagg ccagggggct aaggcactgg tatgtaaagt
     2941 caaaggcaat ggcgtaaatc aacatgttct ggcgatcctc gctgcggatc aacaggccga
     3001 tctgagccaa ctcgccagcc atataggcgg attacgcgct tcgttggcca gcccggcgga
     3061 agtcgatgaa ctcacaggct gtgtcttcgg cgcgatcccc cctttcagct tccatccaaa
     3121 actcaaactg gttgccgacc ccctactctt cgaacgattt gatgaaatcg ccttcaatgc
     3181 aggcatgctg gataaatccg ttattttgaa aaccgcggat tatttgcgca ttgcgcaacc
     3241 agaactgatc aatttccgcc gcactgcgta actagcctgt ccgttcaata agcagaacgg
     3301 acaaaaccag cacaacagca atggcaaaaa acgatgacgt gataatcagt gtctcgacga
     3361 acatttgatc gttcatagca tgcccccggg agatagctgt tttcacgtta ttgttagcgt
     3421 gcacaaatgg cagtttgatg acagttcgcc tgatttttta tgcaaaaaac gtaaagattt
     3481 tctacttcct tcctgcagca agcgtaaagt aagcaggctt attatttttt ggcaaggaaa
     3541 ccacgatgtt tgatgtcact ttgctgatcc tgctcgggtt agctgcgctg ggctttatca
     3601 gtcataacac cactgtcgcc gtttcaattc tggtgttgat cattgtccgc gtcacaccgt
     3661 taagcacctt tttcccgtgg attgaaaaac aggggctaag tatcgggata atcattctga
     3721 cgattggcgt tatggccccc atcgccagcg ggacgctacc gccctcaacc ttgattcact
     3781 cgtttctgaa ctggaaatcg ctggtggcaa ttgcagtagg cgttattgtc tcctggctgg
     3841 gtgggcgcgg cgtgacgtta atgggcagcc agccgcaact ggttgctggg ctgctggtgg
     3901 gcactgtttt aggcgtagcg ctgtttcgcg gcgtaccggt tggcccgctt attgccgcgg
     3961 gtctggtttc gctgattgta gggaaacagt agttaatccc gcaatatatc ggcctggcgt
     4021 ttgccccagg cctttcttaa acatagtaat aaaggccgtt gttgaatcat accccagcgt
     4081 atgagcaact ttctgtacag tatcgccttt cactaatccc tgaagcgcca caatcagctg
     4141 taactgctgt cgccactggc gaaaactcaa ccccgtctct tttactatca gtcgggcaag
     4201 attgcgttca ctcatcgcaa aaaagccagc ccattgcccc aatgaccccc actcgacggg
     4261 ctctttcgcc atcatctcca ccatcgcgcg gattttagga tgagaagaaa cgggtaggtg
     4321 cagctgttgt tgtggctgct gagggagttc atcaaacagg acctgaatta atctgcgggt
     4381 catcggctca gcacgctgag tcgtcgtcct gtttgccagg gttaaaatta attcccggca
     4441 taagggcgat attttcaatg tgcaacaggt tgtcggcatt gtcactgcgc tgggttcgat
     4501 aaacagaaaa cagagttcag cgttagctgt cacttgattg ctatgttcca ccccgcccgg
     4561 tatccaaacc gcatattgtg gcggcaccat ccacaaagca ttttccaccg tacaggtaat
     4621 tgcaccatgt agcgccagta tcagctgtcc tttgcggtgc tgatgtacag gactggttaa
     4681 ctcatccgtt ccggcgtgaa tacaaaatgc gaccgcggct tcatggtgtc ggtcgggttc
     4741 atagccattg agattcaacc tgtgcatcat tttgtccgaa tttagcgata atttgtcatt
     4801 ttagcttgat tcaacataac aataaaaacg gtaaggtaca gcctcgtttg taacaatgag
     4861 aagcatatga cctgttcaac ttcattaagc ggcaaaaaca ggattgtcct tatcgctggc
     4921 attctgatga ttgccactac attacgcgtc accttcaccg gcgcagcacc gttactggat
     4981 acgattcgtt ccgcttactc gctgacgact gcgcaaaccg gcttattgac caccctgcca
     5041 ttattggcct ttgcgctaat ctcacctctg gctgccccgg tggcgcgacg ttttggtatg
     5101 gaacgtagcc tgtttgccgc gttacttttg atctgtgcag gtatcgcaat tcgctctctc
     5161 ccttcgcctt acttattatt tggcggtaca gcggtcattg gcggtgggat tgcattaggt
     5221 aatgtcttac tgccaggatt aattaaacgc gatttccctc attccgtcgc cagacttacc
     5281 ggcgcatatt ccctgacaat gggagctgcg gcggccctgg gatcggctat ggtagtgccg
     5341 ctggctttga atggttttgg ctggcaaggc gcgttgctca tgctgatgtg ttttcctctg
     5401 ctggctcttt ttttatggct gccacagtgg cgaagccaac aacatgcaaa tttgagtacc
     5461 tcgcgcgcct tacatactcg gggtatctgg cgttcgccgc ttgcctggca ggtaacattg
     5521 tttcttggga tcaactcact gatctattac gtaattattg gctggcttcc ggcgatcctc
     5581 atcagtcacg gctataacga agcacaggcg ggttcactgc atggtttgct gcaactagcc
     5641 acagcagctc ccggtttgct gatccctctt ttcttacatc atgtgaaaga ccagcgtggt
     5701 attgcagcgt tcgttgcctt gatgtgcgca gtgggcgcgg ttgggctctg cttcatgcca
     5761 gcgcacgcga tcacctggac tctgcttttc ggttttggtt ccggcgcaac aatgatactg
     5821 gggttgacgt tcattggtct gcgggctagt tctgcgcatc aggcggcggc actctcgggg
     5881 atggcacaat ccgtcgggta tttgttggca gcctgtgggc cgccgctgat gggtaaaata
     5941 cacgatgcta acggtgactg gtccgtacca ctaatgggtg ttgcaatact ttcactactg
     6001 atggcgattt tcggactttg cgccgggaga gacaaagaaa ttcgctaata tccggtgcta
     6061 tagtgacgtg acaaatcatg cgtgaaaggg agaacaaaca cgatgaatat tcagtgcaaa
     6121 cgcgtttatg atccggcaga acagagcgat ggttatcgcg tactggtcga ccgcctctgg
     6181 ccgcgcggta tcaaaaaaac cgatttagcc cttgatgagt gggataaaga aatcacgccg
     6241 tcaacggaac tgcgcaaagc ctttcacggc gatgtcgtcg attttgcaac ctttcgcgag
     6301 caatatcttg cagaactggc acaacacgag caagaaggaa agcggctggc ggacatcgcc
     6361 aaaaaacagc cgctgaccct gctctactca gcaaaaaaca ccacgcagaa ccatgcgctg
     6421 gtgctggccg actggctacg tagcttgtga ttttagtgca gcatccggcg gttatttttc
     6481 accagccgga tggtcccgtc gccacaatgc ccattcatca atcgtttcac cgccgggtaa
     6541 tttgcaattg ttgctgaccc cttgcgctgt ctgcactgga atgagcgtcc cgcccttctg
     6601 ctggcaatag accgacgccg gatttgccat accaatctgc ggcggtttgg gtgcttctgg
     6661 ctgagaaggg gttgaacaac cagccaggac cagcaagcaa ggcagaacaa aactgataat
     6721 tttcatttat tgatctcaca tatgtatcca agcttagagt atcgcggtat cgttttgttt
     6781 ttcagcacta tttttattac attcactcaa aacatattac gccttgtttc atctttgttg
     6841 atgatgtttt atcatgcctg caaagattaa ataatcagca tttacccgcc gtatcctgga
     6901 gttgtttcgt gtcagatcag attatcgccc gcgtctcgca atcccttgcc aaagaacagt
     6961 cactggaaag tctggtccga cagcttctgg agatgctgga aatggtcact gatatggaat
     7021 caacctacct gaccaaagtg gatgtcgaag cgcgcctgca gcatattatg tttgcccgta
     7081 acagccagaa aatgcacatc ccggagaatt ttaccgtctc gtgggattac tcgttatgca
     7141 aacgcgccat tgatgaaaac tgctttttca gcgatgaagt ccccgaccgt tggggtgact
     7201 gtattgcggc acgcaatctt ggcatcacca catttctgag cacgccaatt cacttaccgg
     7261 atggatcatt ctatggcacg ctttgcgccg ccagcagtga gaagcgccag tggagtgaac
     7321 gcgcggaaca ggttttacag ttattcgccg gactgattgc acaatatatt caaaaagagg
     7381 cgttggttga acagctgcgc gaagccaatg ctgcgctgat tgcgcaatcg tataccgact
     7441 cgttaaccgg gctaccgaat cggcgggcga tttttgaaaa tctgacgaca ctgttttccc
     7501 tcgcccggca tcttaaccat aagataatga tcgcgtttat cgatctggat aacttcaaat
     7561 taatcaatga tcgttttggt cataatagtg gcgatctgtt tctcattcag gttggcgagc
     7621 gccttaatac gctccagcaa aatggcgaag ttattggtcg tctcggtggt gatgagtttt
     7681 tggttgtttc actaaacaac gagaatgcgg atatttcgtc gctgcgagaa cgtattcaac
     7741 agcaaatacg tggagaatat cacttaggtg atgttgattt gtattatccc ggtgccagtc
     7801 ttggcatagt agaagtcgat cctgaaacga ccgatgcaga cagtgccctg catgctgccg
     7861 atatcgcgat gtatcaggag aaaaaacaca aacagaaaac accttttgtc gcgcatccag
     7921 cgctacattc ctgaggcgta ttcacatcct tttgattggt gataacatgc gaatcggtat
     7981 tatttttccg gttgtaatct tcattacagc ggtcgtattt ttagcatggt tttttattgg
     8041 cggctatgct gccccgggag cataaagatg aaaaaaacaa cgattattat gatgggtgtg
     8101 gcgattattg tcgtactcgg cactgagctg ggatggtggt aacgtcacct ctaaaaaata
     8161 gcaaaggctg cctgtgtgca gcctttgtgc aatttaagcg ttaactttta atcttcctgt
     8221 agataaatag cacgacaatc gcaccaataa cggcaaccac gaagctgcca aaattgaagc
     8281 catcgacttt accaaagcca aacagcgtgc tgatccaacc gccgactacg gcaccgacta
     8341 tccccagcag gatagtcata aagaatccac caccatcttt acctggcatg atccacttcg
     8401 ccagaatacc ggcaataagc ccaaaaataa tccatgacag aatgcccatt gtttcctcac
     8461 ttatctgttt tgcattagcg ggttagtcgc tgataaaaag catagcacaa catcgggagg
     8521 gcaagatttg tgacgagcat cacggaggat ttttttgcga tggcgcagaa attgcgccat
     8581 caacgatcag tgataattac caaccacaaa catcatgttc gttttccgtg tcataagaac
     8641 gcacggtatt caccagatct tttatcactt cagccgccac ttctggcacc agcaaagtca
     8701 tcggcgtctc tgtttcataa tcgacagaaa cgccattgct gttattggtg acggtcacgg
     8761 tatacgttgc tttacccatg attcatttcc cgttatgaat gactttccgt tgttgcgcac
     8821 cttccatcag gacttcagga gccacgaaga agtcaatgtt gaaataagta tcgtcagtca
     8881 tggcttcaat gttgtgccac ttttctggag ggaacaccgc aaactgcccc gcttcgataa
     8941 ggatcacctg atcaggctct gcactgtgtt catcagcgta gccgagatat ttgaccgccc
     9001 catgcataac ggaaaggcgt gggtaaaccc ccgggcgcgt tcctttatca agatgacgtt
     9061 cgaatatgcc ggcaggcgca gtttgtttat tccagaaagg cgttgagcgc gtatgaatat
     9121 aattctgtgg gatttgcagc atccttttcc ctccttcggt gaatgcgctg aaaacggttt
     9181 attccagccg tttcagggta cgcccgataa tttgcatttt aaataccatt tattggttac
     9241 tttttagcac catatcagag aagaatcagg gaggattata gatgggaagc ccgcgacaac
     9301 gcgggctgaa agcatcagga ttgcagcgtc gccagtcggg cagcgaaacc cacgaacatc
     9361 aaaccaatca gtgagttgcc aactttagcc agtttctttt tggtacgtat gtactgcgtg
     9421 acaaaagcac cagatataat caggaagctc aaatagcaga aactcaccag ttccagcgtc
     9481 gtcgcaagaa taaagaatga aattcccgta tgtggggcat taacatcgat aaactgtacg
     9541 aaaaacgaca catagaacaa aatggctttc ggattagtca ggctcaaaat taacgcgcgt
     9601 ttaaaaatag caccgtattg gggctcatcg gatttggcct cgctattttt acccttcagg
     9661 gtcgcgtaaa gaattttact ccccagatag agcaaataaa acgcaccaag ataacgtaca
     9721 atgttgaata atatcggggt ggtcttaatt aatgtcgcca ctccagccca tgccagaaac
     9781 atcaataccg catcgccaat aaatacaccg caggccgcaa gataaccgcc tttcataccg
     9841 ctactgacgc tatttttgag tacaaacagg gtatttggcc ctggcaccaa cacaataaaa
     9901 atggccccaa ccagataggt ccagtaattc agaaccccgt attcagcgaa cacattaacc
     9961 tctttaatta tctttcgatc atgcgcgatt aaaggtgaat atgctaacca atctgtagcg
    10021 gcttagaaag gagaaaatca ggttttaacc tgatatcaac ccgataattg aatcattaat
    10081 caggcatgct ccagtgaaaa attcgggtag tgctcggcaa aatactggcg taaaaattct
    10141 accgttatcc gcactttcgc tgacgtcgcc agccttgaaa cataaacggc ccagacgttc
    10201 gctggctggt aatattccgg tagcacttgc actaaatgac cactggcaat gttttcgcta
    10261 acatcccacc aggaacgcag agcaatcccc tgcccatcga gacaccactg atggacaatt
    10321 tctccatgat tagacgacag tgggccggtg actttaatcg catggtggcc ttctttattg
    10381 cgtaattgcc agacaccaaa aggatgatcg cgctctttaa tgaccaggca cggcaatgca
    10441 gaaagatcgg ttaaatgttt cggcgcgcca tgttgcgcaa taaattccgg cgacgcgcag
    10501 agaatgcggt aattggtcgc cagtttgcgg gcgattaaat taggggcgat gtcatcgcca
    10561 atgcgaatat cgagatcgac accttcattg accaaatcga ccagccggtc ttccacatca
    10621 aaacgtaatt ccagttgcgg atacgccttc gccagcgctg ataacgccgg agccaccacc
    10681 tgtcgtccaa aaccaaagct gctgataata cgcagcatcc cctgtggcac ctgacgcacg
    10741 tcagaaagtt cgtccatcat ctgaccgaca tcctgcaaaa tccgctgcgc ccattcataa
    10801 atccgctctc cttcttcggt aatggtgacg cggcgggtgg tgcggtgtaa caacaccacg
    10861 tttagcgttt gctccagcaa ggcgatgcgc ttgctgacga acgccggtga aacgcccagt
    10921 tcttccgcca cggcggcaaa accagcccga cgagccacca gcataaagac gcgcaaatca
    10981 ttcagcagcg gtaaattatt catgattcgt gttttatgtt tcaccagtta cggggattaa
    11041 ttccttttca gtcaattata ggatggtgat gttgtcaatt ttgatggtca ggaagtgaga
    11101 acccaataat gaaaacgatg cgtattgctg cgatcccggg agacgggatt ggcaaagaag
    11161 tccttcctga agggattcgc gtgttacagg ctgccgctga gcgctggggc ttcgccttga
    11221 gttttgagca aatggagtgg gcgagctgcg agtattacag ccatcacggt aaaatgatgc
    11281 cggacgactg gcatgagcaa cttagccgtt tcgacgccat ctattttggt gccgtcggct
    11341 ggccggacac cgttccggac catatttcgt tgtggggttc gctgctgaaa tttcgtcgtg
    11401 aattcgacca gtacgtcaac ctgcgcccgg ttcgtctctt tcctggcgtt ccctgcccgc
    11461 tggcgggaaa acagcctggc gacatcgatt tttacgtggt cagggaaaac accgaaggcg
    11521 aatattcctc gctcggcggt agagtgaatg aaggtacaga gcatgaagtc gtcattcagg
    11581 aatcggtatt tacgcgtcgt ggtgtcgatc gcattttgcg ttatgccttc gaacttgcgc
    11641 aaagccgccc acgtaagacg ctaacttctg ccactaaatc aaacggttta gccatcagca
    11701 tgccgtactg ggatgagcga gtggaagcaa tggccgagaa ttacccggag atccgctggg
    11761 acaagcagca tattgatatt ctctgcgcgc gttttgtgat gcagccggaa cgcttcgatg
    11821 tggtggtggc gtccaatttg tttggcgata tcctttccga tcttggcccg gcctgcaccg
    11881 gcaccattgg cattgcccca tccgccaacc tgaatccgga acgcactttc ccgtcgctct
    11941 tcgagcctgt ccacggttcc gcgccggata tctacgggaa aaatattgct aaccctatcg
    12001 ccacaatttg ggccggtgca atgatgctcg attttctcgg caatggcgat gagcgtttcc
    12061 agcaagcgca taacggtatt ctggcagcga ttgaagaagt gattgctcac gggccgaaaa
    12121 caccggatat gaaaggcagt gccaccacgc cacaggttgc cgacgcgatt tgcaaaatta
    12181 ttttgcgtta aggtcaaacc agtttatttg aaccgcgtca ctgacgcggt ttttttattc
    12241 gttcttttca gttaataacc tgcgtcattt caccttttat tgtttccgtt tcgtgtttta
    12301 tggctttccg tattcttaat tgtttaattt atgtaacatg caaataatca atttgttaaa
    12361 cacaacatac aactcattac cctgcaaata attacaactg gcaaaagcca gagacgggct
    12421 tttatgccca ggagatgact catgatgagc aatgttaaga aaaaagatgt gccgctgata
    12481 agcatcagcc tggtggccat tcttttcatc gcagctgcat taagcctttt cccacaacaa
    12541 tcggccgacg cggccaacgc aatatacact tttgttacgc gtacgttagg ttccgcagta
    12601 caggtattgg ttttgctggc aatgggactg gtgatttatt tagccaccag taaatacggc
    12661 aatattcgtc ttggcgaagg aaaaccggaa tacagcacgc tctcctggct gtttatgttt
    12721 atttgtgccg gtttaggttc ttctacgctt tattgggggg ttgctgaatg ggcctattat
    12781 tatcaaaccc ctggattaaa tatcgcaccg cgttcacaac aggcactcga atttagcgtc
    12841 ccttactcct tcttccactg gggcatcagc gcctgggcaa cttatacgct ggcctcatta
    12901 atcatggctt atcactttca tgtgcggaaa aacaaaggtc tgagtctttc cggcattatt
    12961 gctgctatta ccggcgttcg cccgcaaggc ccgtggggaa aactggtcga tttgatgttc
    13021 ctgatcgcca ctgtcggtgc gctgaccatt tcccttgttg ttaccgcagc aacctttact
    13081 cgtgggcttt ccgcgctgac cggtttaccc gataacttca ccgtgcaggc atttgtgata
    13141 ctgctttccg gcggcatttt ttgcctaagc tcgtggattg gtatcaacaa cggtttgcaa
    13201 cgtcttagca aaatggttgg ctggggcgcg ttcctgctgc cattactggt gctgattgtc
    13261 ggcccaaccg aatttattac caacagcatc atcaatgcca tcggcctgac cacgcaaaac
    13321 ttcctgcaaa tgagcttatt caccgatccg cttggcgatg gttcatttac ccgcaactgg
    13381 accgttttct actggctgtg gtggatctca tacacacctg gcgtagcaat gtttgtcacc
    13441 cgcgtttccc gcggtcgtaa gattaaagaa gttatctggg gactgatcct cggcagcacc
    13501 gtcggttgct ggttcttctt cggcgtaatg gaaagctatg ccattcacca gtttatcaat
    13561 ggtgtaatca acgtcccaca ggtgctggaa acactgggcg gcgagacagc tgtgcagcaa
    13621 gttctgatgt cgttgccagc cggtaaattg ttcctcgccg catacctggg cgtgatgatt
    13681 attttccttg cctcgcatat ggatgcagtg gcctacacca tggcggcgac cagtacgcgt
    13741 aatctccagg aaggtgacga tcctgaccgt gggctgcgtc ttttctggtg cgtggtgatc
    13801 actctgatcc cgctttccat cttgtttacc ggtgcttcgc tggaaacgat gaaaaccacc
    13861 gtcgtgctca cagcccttcc cttcctcgtc attttactgg tgaaagtcgg cggatttatt
    13921 cgctggctga aacaggatta cgccgacatt ccggctcatc aagttgaaca ttatctcccg
    13981 cagacaccgg ttgaagccct ggaaaaaaca ccagtgctcc ctgcgggaac cgtattcaaa
    14041 ggcgacaact gagcgccatc atcctaacga taaaggtatc cctatgagca atctgagccc
    14101 tgactttgta ctacccgaaa atttttgcgc taacccgcaa gaggcgtgga ccattcctgc
    14161 ccgtttttat accgatcaga acgcgtttga acacgaaaaa gagaacgtct tcgccaaaag
    14221 ctggatttgc gtcgctcaca gcagcgaact ggcgaatgcc aatgattatg tgacgcgtga
    14281 gatcattggc gaaagcatcg tgctggtacg cggtcgtgat aaggttttgc gcgcgttcta
    14341 taacgtgtgt ccgcaccgtg gtcatcagtt gttgagcggt gaaggaaaag caaaaaatgt
    14401 gattacctgc ccgtatcacg catgggcatt caaactcgat ggcaatctgg cccatgcacg
    14461 taactgcgaa aacgtcgcca atttcgatag cgacaaagcg caactggttc cggtgcgtct
    14521 ggaagaatat gccggattcg tcttcatcaa catggacccc aacgccacca gcgtagaaga
    14581 tcagttaccc ggtctgggcg cgaaagtgct ggaagcctgc ccggaagtcc acgatctgaa
    14641 actggcggcc cgctttacca cccgcacgcc tgccaactgg aagaacattg tcgataacta
    14701 tctcgagtgc tatcactgtg gtccggcgca tccaggtttc tccgactccg tacaggttga
    14761 tcgttactgg cacaccatgc acggtaactg gacgctgcaa tacggtttcg ccaaaccgtc
    14821 cgaacagtcg tttaaatttg aagagggtac ggatgcggca ttccacggtt tctggctgtg
    14881 gccgtgcacg atgctgaacg tcaccccgat caaagggatg atgacggtca tttatgaatt
    14941 cccggtggat tctgaaacta ccctgcaaaa ctacgatatt tacttcacca atgaagtgtt
    15001 aaccgacgag caaaaatcgc tgattgagtg gtatcgcgat gtgttccgtc cggaagattt
    15061 acgtctggtt gaaagcgtac agaaagggct gaaatcgcgt ggctatcgtg gtcaggggcg
    15121 catcatggcc gacagtagcg gtagtggcat ttccgaacat ggtatcgccc atttccataa
    15181 tctgctggcg caggtgttta aggactaatg acatcggcgg cggtattttc cgccgctggg
    15241 ctgatttttg atggagtaca gcaatgtcag actatcaaat gtttgaagta caggtgagtc
    15301 aggtggaacc gcttaccgaa caggtgaaac gcttcacgct ggtggcaacc gatggcaaac
    15361 cgctgcctgc gttcaccgga ggaagtcaca tcattgtgca gatgagcgat ggtgataacc
    15421 agtacagcaa tgcgtattca ctactgagtt cgccgcatga cacctcttgt tatcagattg
    15481 ccgttcggct ggaggaaaac tcgcgcggcg gttcccgctt tttgcatcag caggtaaaag
    15541 tgggcgatcg gttaacgatt tcaacgccta ataacctgtt tgcgctaatt ccctcagcca
    15601 gaaagcatct gtttatcgcg ggcggtattg gtatcacccc tttcctgtcg cacatggcag
    15661 agctgcaaca cagcgacgtc gactggcagc tacattactg ctcgcgaaat ccagaaagtt
    15721 gcgcatttcg tgatgagcta gtccagcatc cgcaggctga gaaagtccat ttgcatcatt
    15781 catcaaccgg aacacgactg gaattagcgc gattattggc ggatatcgaa cctggcacac
    15841 acgtttatac ctgtggcccc gaggcgctaa ttgaagcggt aagaagtgaa gctgcgcgtc
    15901 tggacatcgc cgccgatacg ctgcactttg agcaatttgc tatcgaagac aaaaccggcg
    15961 atgcatttac cctggtgctt gcccgttccg gaaaagagtt tgtggtgccg gaagagatga
    16021 ctattttgca ggttattgaa aataataaag ccgcgaaagt ggaatgttta tgtcgtgaag
    16081 gggtatgcgg aacctgcgaa acagcaatac tggaaggtga agctgaccat cgggatcaat
    16141 attttagcga tgaagagcgt gccagccagc aaagtatgtt gatctgttgt tcgcgtgcga
    16201 agggtaaacg cctggtgttg gatttgtagt ttgccgaagc cggatgtggc gctgagcgcg
    16261 cccagtccgg cttcggaaga ttttactgcg gatattcctg caataaattg tgtaatgctt
    16321 ccgccatcag ctcaccacgc cagccggaaa tcagctccgg caaattgttc tgcggtttca
    16381 gtttccagtg ccagttcagc agttggttga tttgccgacg cgatgccagc aattcggcgc
    16441 tgatcttatg cgtttcgctc acgtcagtaa tcagcgactt aatcgcttta aacgctttac
    16501 gataacccgg catgtccatc aggttaagca tcggctgcgg taaggcatct tccggcaatg
    16561 tctgcgcttt ttccaccagc gccagcagcg ttttaccgtg aaagcggatt tcgctaccgg
    16621 ataaacccag gctgtccagt tcgcctaaac ttcccggcat ataacgcgct accgaccaca
    16681 aatgctcttc acgcacgaca aagttcaccg ccagatcgcg ctctcgcgcc ttgcgcagtc
    16741 gccagtcggc taacagttgc agacaggcca gttggcgtgt gcgtaattgc caggcattgg
    16801 tgatatcacg ccaggcatct tccggcgcaa cgacttcctg acgacgcatt tgcatcaggc
    16861 ggcattcatc cagcgccgca ggtagccagc cggaggcctc cgtttctacc ataagcttgg
    16921 cggtgatcgg taacagatac cagacatccg ccgctgcgta ttcacactga cgttcggtca
    16981 gcggtctggc cagccagtcg gtgcgcgatt cactcttgtc cagcgtaacg ccggaatact
    17041 cttccaccat ggaagcgaaa ccccatgaca tcgggcgtcc gcagaaggca gcaaggattt
    17101 gcgtgtcaat caagggttgt ggtaattcgc caaagacatt gaggaacact tccagatctt
    17161 cactgcctgc atggagaaat tttgtgatgg acggatcgcg caggatcgct ttcagcggtg
    17221 accagtcggt gatcccgagt ggatcgatta gcgccagatg ctcgccatcg aaaagttgaa
    17281 tcaaccccag ctgcgggtaa taagtgcgcg tacgaacaaa ttcagtatcc agggctatcg
    17341 ccggaaaggc acggacggct tcacacaaag aagccagcgc atcgtccgtg gtaatcattt
    17401 ggtaattcaa attgttttct ctttagtggg cgtcaaaaaa aacgccggat taaccggcgt
    17461 ctgacgactg acttaacgct caggctttat tgtccacttt gccgcgcgct tcgtcacgta
    17521 attctcgtcg caaaattttt ccgacgttag atttcggtaa ctcatcacga aactccacca
    17581 gcttcggtac tttgtatccc gtgagctgac ggcggcaaaa agtcaccagt gactcttcgg
    17641 taagcgatgg atcttttttc actacgaaga ttttcaccgc ttcaccactg gagccggaag
    17701 gtacgccaac agccgcgacc tcctgtacgc caggatgctg catgacgaca tcttcaatct
    17761 cgttgggata gacgttaaaa ccggaaacca gaatcatgtc ttttttacga tcaacaatgc
    17821 gcaggaatcc ttcttcatcc attaccgcga tgtcgccggt gtgtaaccag ccatttttga
    17881 tgatttcatc ggtagcatcg ggacgctgcc agtaacccag catcacctgc ggtcctttga
    17941 cacaaagctc acccggttga cctggtggta cttcattatc atcatcatcc accagtttgg
    18001 cttccgtcga cggcaccggc aaaccgatgc taccactatg ataatcaata tcatatgggt
    18061 taacgctgac cagcggagca cactcggtaa ggccatagcc ttccagcaga tactgtcctg
    18121 tcagtttcac ccaacgctct gccaccactt gctgcactgg catccctccg cctgcggaaa
    18181 gatgcagact ggagaaatcc agctgctgga actctttatt gttcagcaac gcattgaaca
    18241 aggtgttaac gcccgtgata gcggtaaacg gatatttcgc taactctttt accaaccctg
    18301 gaatatcgcg cgggttagtg ataagcaggt tctgcccacc cagttcgata aacagcaggc
    18361 agttaatggt cagggcaaaa atgtgataca gcggcagtgc cgttactacc agttctttac
    18421 ctggatgcag caacggccca taggtggcgt tgacctgttc caggttcgcc agcatattgc
    18481 ggtgagtcag catcgcgcct ttcgccacac ctgtggtgcc gccggtgtat tgcaggaagg
    18541 ctaaatcttc cggcaccagt tcgggtttga cgtactgcat ccggtagccg ttatgcagtg
    18601 cgctacgaaa tgaaatggca tctggcagat ggtatttcgg caccaaacgc ttgatgtatt
    18661 taacaacgaa attgactacc gtgccttttg ccgtagatag ctgatcgccc atacgggtca
    18721 gaattacgtg ctgaacggcg gttttatcaa ccactttttc cagtgtgtga gcaaagttag
    18781 acacgataac aatcgccgat gcgccgctat cgttaagctg atgctcaagc tcacgcgggg
    18841 tatacaacgg gttaacgttt acgacgatca tcccggcacg caaaatgcca aacagcgcca
    18901 ccggatattg caataaatta ggcatcatca acgcaacgcg atcgcctttc ttcagcccca
    18961 acccttgttg caaataagcg gcaaacgcgc gactgcgttc ttccagcttg cggaaggtca
    19021 ttacctcccc catattcaca aacgcaggct gatcggcgta gcgcgcgacc gactgctcaa
    19081 acatatctac cagagattga taacggtcag ggttgatctc cgtcggaacg tccgcgggat
    19141 aacggttaag ccaaaccttc ttcaattctt cacctctaaa atgcgtgttc gtcgtcatcg
    19201 caaccccaaa tgatatacat gccgttaaca taatattaac tcatcatacc agcttgataa
    19261 ttacccaacg aaaaggttgc gaagcgcgtc actatttatt tttatcttta ccgtaagaat
    19321 gcagaaacag cggaccagcc gctgtttctt tttctttaaa aacaagcaat tattcagtta
    19381 caactgtttg tactctcgcg gggccgggat tataccagcc ccatccgccg tagccatagg
    19441 gccagccacg accgccataa aaccagggat caatcggctg aggcggcata atcacctgct
    19501 gggttaaatg ccaacgtttg taacccgtta cttgcatcac cataaattta tagggcgtat
    19561 tgccgatttt gccgtcaaca gcaccagtga ttggcccgac taccgtaacc agttgtccac
    19621 ggaaatccac cgggtccaga aaaccgttca catcggcata aatgcgaccg cgagaaggtt
    19681 ctcccagcgt cggtctggct ccgctgtcca gcggtacggt agcaatttcc aggcgggttt
    19741 tcccttgctg gttttgtacc gcaaccactt tgccaccaaa gcgtgcctcc tgaccaacgt
    19801 acagctgcgg cgcactcatc acccgaacta aatcttgttg cggcgtggga ctgctgcctt
    19861 taatggcgtc cggcacagtg acacaaccgc tcagcattag cgcaaacgta cctgccagta
    19921 tgcctttgat aacattcttt tgaaccgcca tgatgcgact cctttttttc tcagggcata
    19981 ctcttaagat tcattctttg cccggaagtt tcttccatgc gacgttgtta cgtaaataaa
    20041 ccggttcggc atgttccacc gccaccgttt taccctcggc aaacatctga caagcaatcg
    20101 gcagcatatc ttctgcagcg ggcagtaaaa cttctccgtc gcgcaaaacc agtccgctct
    20161 ctttaccgag atccggccag gcttgccagc ctgtacctac cgtcacccat tccccggaaa
    20221 gctgttgcat tcgttcatga acgagttcgg gtttgagtac ggcttcggtt tcttcaccgt
    20281 gccagatacc gttttcatca cgctgatatt cggcccagta aacttcgccc attcgcgcgt
    20341 caatggctga cagcacgcgt gttgcgccgt ttttgcgcca cgccccttgc gccatcgtca
    20401 ttagcgtgga gacgccaatc atcggtaatt ccgcgccaag cgccagccct tgcgcgatgc
    20461 caataccaat gcgcacgccg gtaaagctac cggggccgcg cccgtaagcc agagcgttaa
    20521 tatcagtcag ggaagttccg ctggtggtca ggatatcctg caccatcggt aagattcgtt
    20581 gagtatgttc acgagggcaa agctcaaaat gagcgttgac agtaccgtcg ttccacaggg
    20641 cgacagagca ggcctctgtc gcggtatcga tagccagaat tcgcatgggt cttcgtgctt
    20701 agatcaataa aaaggcgcgc atcataccat actccgtaac aaattacctg gaggatggta
    20761 tcgcaaggaa acgaaccgca cgggcaatgt cacgggtgcg tggcgcgggc ggcagactgg
    20821 cgagaaacgt cgcgccgtaa ggacgcatca ccagccggtt gtcacaaatt accaaaaccc
    20881 cgcgatcgtc ggcgtcgcga atcagtcgcc cgactccctg cttgagagta atcaccgcat
    20941 ccggcagttg tacttcatcg aacgggtcgc caccgcgcaa acggcaatct tccatgcgcg
    21001 cttttaacag tggatcatcc ggcgaggtaa acggcaattt gtcgataatc accaatgaca
    21061 atgtatcgcc acgcacgtcc accccctccc agaaactact ggttgccaca agaagcgcgt
    21121 taccggcact gacaaattgc tgcaacagtt gccctttgct ggtttccccc tgcaataata
    21181 cgggaagcgt catggtagcg cggaactgct cggccagatc gcgcatcatg gcgtgcgagg
    21241 tacaaagcat aaaacagcga ccgttgttag cttcgatgat cgggcgcagc atagccgcca
    21301 gttgccgcgc ggaacccggt tgattggttt gcggcaggtt gcgcggcaca cagagtaacg
    21361 cctggcggct gtaatcaaac gggcttggca atagcaacga ctccgcctgt tctatcccaa
    21421 gccgcgaggt gaaatgatgc agatcgtcgt tcaccgacag cgttgctgag gtgaagatcc
    21481 agctaccggg cttttgcgcc attaactctt tgaatttatc cgccacgctg agcggcgtga
    21541 gagccagagt aaaatggcgc gaagtgcatt cgtaccagta gctgtagccc ggctgattga
    21601 tctcttttag ccgcttcagc cgtgtgcgat acaacgtggc gcgctcaaat gccgcatcca
    21661 gcaaggcgga acgccccagt gacagtttcg ccacgtcatt acaaagttcc agggtgtcat
    21721 cgagcagtaa aaatgcccgc tgaatttgcg gattagctaa cagctcacgc aggttaccgc
    21781 gataacctgg ttcaccgagt tgcagacgaa aatcctgcgc actctgggca agacgatcag
    21841 cgcacttttg taactgctgg gtgtctttta attcggtgcg gtaggcgatg gtgatgtctt
    21901 ttgccaggtc gagcagttgt cgactggaga gtgactgacc aaaatactgg ctggcaatgt
    21961 ccggtagctg gtgggcttcg tcgaagatca tgacgtccgc ttccgggatc aactcgccaa
    22021 atccactctc tttaaccacc atatccgcca gaaagagatg atggtttacc accaccacat
    22081 cggcgtccat cgctttttta cgtgctttga ccacaaagca atctttatac atcgggcagt
    22141 cgctgccaag acagttgtcg ttggtgctgg tgaccagcgg ccacgcctgt gaatcttccg
    22201 ccacgctgac gcaggtgctg atatcaccat cgactgtttg attagaccag gagcgcagca
    22261 ggatcacatc acttaagatt tgtaccggca gatcgccccc cgccagcgcc tgctgttcga
    22321 gacgctcgag gcagaggtag tttgagcgcc ctttcagcag cgccacgttg cccgtatatt
    22381 tcaatgcctt tgagactgtt ggcaaatcgc ggctgtagag ctgatcctgc aacgcttttg
    22441 agccggtcga gataatgact ttctttttcg cccgcagcgc aggagccagg taagcgtagg
    22501 ttttgcccgt accggttcct gcttccacca ccagcggctg gcctttttct atcgcctggg
    22561 tgacggctac cgccatctgt cgctgtggtt ctcgcggctt aaagcctggt atcgctttcg
    22621 ccagctgacc gtctggtgca aaatcgtccg tcacactacc ccctgttgat ttgaacaggg
    22681 attatgtcag gatgagggcg ctttcgccag ttgaagtggt gacggcgacc tcacattgtg
    22741 gcagtctttg cagcacgaaa atggaaggat aatgaggaca aaatgatgac tatcgttcgt
    22801 atcgatgctg aagcccgctg gtctgatgtg gtaatccaca acaacacgct ctactacact
    22861 ggtgtaccgg aaaacctcga cgccgatgcc tttgagcaaa ccgccaacac gctggcgcag
    22921 attgacgccg tgctggaaaa acagggcagc aataaatcga gtattctgga tgccaccatt
    22981 ttcctggccg ataaaaacga cttcgcggcg atgaataaag cgtgggatgc ttgggttgtc
    23041 gcgggtcatg cgccggtgcg ctgcacggta caagcgggtt tgatgaaccc gaagtataaa
    23101 gttgaaatta agattgtggc tgcggtgtaa gctttatcga agcaaaataa gtcagacgat
    23161 aatttatcga taatactggt cggttttaca taaatcga
//
LOCUS       DAPGOF010000072        23132 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.072, whole genome shotgun sequence.
ACCESSION   DAPGOF010000072 DAPGOF010000000
VERSION     DAPGOF010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23132
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.072"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            22..582
                     /locus_tag="RHV13_002413"
     CDS             22..582
                     /locus_tag="RHV13_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDV1241940.1"
                     /translation="MQGHRIGYVRVSSFDQNPERQLEQTQVSKVFTDKASGKDTQRPQ
                     LEALLSFVREGDTVVVHSMDRLARNLDDLRRLVQKLTQRGVRIEFLKEGLVFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKALSDEQAATLRQRATA
                     GEPKAQLAREFNISRETLYQYLRTDD"
     gene            586..3552
                     /locus_tag="RHV13_002414"
     CDS             586..3552
                     /locus_tag="RHV13_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs1 family transposase"
                     /protein_id="HDV1241941.1"
                     /translation="MPRRLILSATERDTLLALPESQDDLIRYYTFNDSDLSLIRQRRG
                     DANRLGFAVQLCLLRYPGYALGTDSELPEPVILWVAKQVQAEPASWAKYGERDVTRRE
                     HAQELRTYLQLAPFGLSDFRALVRELTELAQQTDKGLLLAGQALESLRQKRRILPALS
                     VIDRACSEAIARANRRVYRALVEPLTDSHRAKLDELLKLKAGSSITWLTWLRQAPLKP
                     NSRHMLEHIERLKTFQLVDLPEGLGRHIHQNRLLKLAREGGQMTPKDLGKFEPQRRYA
                     TLAAVVLESTATVIDELVDLHDRILVKLFSGAKHKHQQQFQKQGKAINDKVRLYSRIG
                     QALLEAKESGSDPYAAIEAVIPWDEFTESVSEAELLARPEGFDHLHLVGENFATLRRY
                     TPALLEVLELRAAPAAQGVLAAVQTLREMNADNLRKVPADAPTAFIKPRWKPLVITPE
                     GLDRKFYEICALSELKNALRSGDIWVKGSRQFRDFDDYLLPAEKFAALKREQALPLAI
                     NPNSDQYLEERLQLLDEQLATVTRLAKDNELPDAILTESGLKITPLDAAVPDRAQALI
                     DQTSQLLPRIKITELLMDVDDWTGFSRHFTHLKDGAEAKDRTLLLSAILGDAINLGLT
                     KMAESSPGLTYAKLSWLQAWHIRDETYSAALAELVNHQYRHAFAAHWGDGTTSSSDGQ
                     RFRAGGRGESTGHVNPKYGSEPGRLFYTHISDQYAPFSTRVVNVGVRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGETKLYVPQGVQAYPTLRP
                     LIGGTLNIKHVRAHWDDILRLASSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNSLARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERATQGLVEAGKPVDGELLQFLSPLGWEHINLTGDYVWRQSR
                     RLEDGKFRPLRMPGKP"
     gene            complement(3604..3744)
                     /locus_tag="RHV13_002415"
     CDS             complement(3604..3744)
                     /locus_tag="RHV13_002415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1241942.1"
                     /translation="MKDENTPKIGAYCGLDGQNCQPCIVSRVLLIIVVGYALFYLFAA
                     LK"
     gene            complement(3776..4243)
                     /locus_tag="RHV13_002416"
     CDS             complement(3776..4243)
                     /locus_tag="RHV13_002416"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1241943.1"
                     /translation="MNRYLKSMAAGFIATIVLSALMVMKSSMGIMPELNPVGMLSGMA
                     HSMMGISAGLNIGWLAHFMIGTVLWGLLFALLYNRLPGQSATKKGLMFSVLAWLLMML
                     IPMPMAGAGFFGITLGIMAPVMTLILHLIWGAVLGYTYGRMTTSPLTQGHPAE"
     gene            4441..4917
                     /locus_tag="RHV13_002417"
                     /pseudo
     CDS             4441..4917
                     /locus_tag="RHV13_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003935408.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            5048..6052
                     /locus_tag="RHV13_002418"
     CDS             5048..6052
                     /locus_tag="RHV13_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS5075 family transposase"
                     /protein_id="HDV1241944.1"
                     /translation="MENIALIGIDLGKNSFHIHCQDHRGKAVYRKKFTRPKLIEFLAT
                     CPATTIAMEACGGSHFMARKLEELGHFPKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTESQQAMRALHRVRESLVQDKVKTTNQMHAFLLEFGISVPRGAAVISR
                     LSTLLEDSSLPLYLSQLLLKLQQHYHYLVEQIKDLESQLKRKLDEDEVGQRLLSIPCV
                     GTLTASTISTEIGDGKQYASSRDFAAATGLVPRQYSTGGRTTLLGISKRGNKKIRTLL
                     VQCARVFIQKLEHQSGKLADWVRELLCRKSNFVVTCALANKLARIAWALTARQQTYEA
                     "
     gene            complement(6329..7717)
                     /locus_tag="RHV13_002419"
     CDS             complement(6329..7717)
                     /locus_tag="RHV13_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1241945.1"
                     /translation="MSQSPYDDEFRAIRYIQLRGQDIANAHETINSDIESLKAQLTGL
                     ISGTELDEAEHLALKEHHLREMTPSDTAMHSTGLKTIYSEANQRVCGDIGLATILSTD
                     DLAVVDARIQNHIKEFNDRYALDAWDYAIACGCGLIASMLDLLCVRAPPKPTVSFTAE
                     VDGIFNKQVQKAFNAILPEDLSTKLSDLFPIGAPDSSISSDLVGAAGGVLSPTNHRLR
                     ALSHDPVLGIIFGIKDMLNGTCTVVQNGQIVVYPSSKGVTDETNIFRLIARMFGHLAS
                     DVNAPSAKGNRGMGLPAPFMGLLRMLEGIPVGSSNFGKQIEYMYVNGYDFRQFIVTSI
                     PMSIMEVLMRVFYVAKQVSLGKGAFGETLLDTMPLRLNPRFRMMLALGYGTSSAVNAG
                     KMYITGNILNANYASWMGLAWNGFHSLKWSLYQRHLKLWAGIEKAELERLQNNIDSIE
                     ALTIRAGNLPVK"
     gene            complement(7722..8219)
                     /locus_tag="RHV13_002420"
     CDS             complement(7722..8219)
                     /locus_tag="RHV13_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015958327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1241946.1"
                     /translation="MDATETEELEKIRKKAMDEVTSALQALENKFPGITEGAAALAGS
                     GVGAAASFGALYTLGTTGVSAAGITSGLATAGSIVGGGMVAGIAVLAAPVAVLGIGGY
                     AIVKHKKNAKLTAALSQAIQKLYEVQERLMSNAEYFKAEIAGIKATIDMLTKKAPKGS
                     LVAVR"
     gene            complement(8422..9078)
                     /locus_tag="RHV13_002421"
     CDS             complement(8422..9078)
                     /locus_tag="RHV13_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HDV1241947.1"
                     /translation="MFENYVCKIYVSNDPHFSSHLEATAFGFDNGQQQKILTAAHVVT
                     NALGKIYPVSNTLKLYVKFLNHQGLVNEEPVLVDFILNEANDRADFKDGIPFVDSAEI
                     VLPAGIQQPVSSYFKVLAPAAGMGTLGVGYPMNETTISTFPGEVSGIWPLNCSNHSPQ
                     HLTTRFVIAHFNTDGCSGGPYVVSENDEHFVIGSLVGIMSGTCPDSNPHMSVQSATDF
                     "
     gene            complement(9737..10129)
                     /locus_tag="RHV13_002422"
     CDS             complement(9737..10129)
                     /locus_tag="RHV13_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000341068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1241948.1"
                     /translation="MDDKEQFTNLVAKHASGLTEEQLAGYDACSLDGECVTPSYEVFR
                     GYRTRHTLDEFLEMAISLNAIHPDEYLTDMLLKPHEVIGALADEGDQLNNATPVYFFP
                     DTGVYAAAVSETRVLDAWLCWPCYPANW"
     gene            11050..12036
                     /locus_tag="RHV13_002423"
     CDS             11050..12036
                     /locus_tag="RHV13_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1241949.1"
                     /translation="MTTQSSSIQYAYALDGEGTLTHIGAALRSHTYTCPGCKSPLTPV
                     MGEFNAKHFRHSEECCALETYLHKCGKEAFFYRYQQALSREMPISLELERRVACNGSR
                     LALVRDEARQCVKSVPARYNLTQFFDQAELEKHDKVTGLRPDVMLYDTTGERRCYVEI
                     CVTHPCSQDKIEAGIPILEFKVQSASDIQMLLTGAYSIKEKILRVFNWLPPFQSVDTC
                     SGVCSVGNVDMSVWSLSGSGRLNEQTMPLAEVDLTINSDVNTWPRSLGAAELADNLRA
                     FIRHADPHSLFPNCIMCEQAGRWEDGYLQCHSKAKIVPYTEARQCANYKVKA"
     gene            12033..14072
                     /locus_tag="RHV13_002424"
     CDS             12033..14072
                     /locus_tag="RHV13_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020803887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDV1241950.1"
                     /translation="MTKRLTWEQKSIVSHDTGHALVKAVPGSGKTTTLVKRVERLVKT
                     GTDPRSILILMYNKSAQVSFTENLKTALMSSVIPEIRTFHSLALKIVGYGERQQIIKK
                     KDLITPSDYRYEQLVKQAYRYGFDHEANYIDPNEIENFELFIARCRAAAVTPVDAAND
                     PTFSNIKREFIHAYGRYCELLEENSLRTFDDCLIEAVALLRNDSSLGAHFKHIIVDEY
                     QDVNLIQHDMTRLLSKSDTSVMAVGDVNQCIYEWRGARPDFIGGLFERHYPNTKVFQL
                     SCTFRFGHELSLMANSVIRRNSTKLTKLCVSHPSTPKTEVRLHFDNCLSKVLSNLSVS
                     SGTQAILSRTKANLAEAEIALRLCGLPYRYLNGSSALHTRTEIGILVVGVLLSVYGDL
                     RLLENHPNKQAIVYGFLKEAGFRWQKGQFKAALSGLMAPHADLWSALGQLFEGAQYQK
                     DRLGRLATICQKDGEETPAIDVLRRLSMEGFIDSVGSEGVTRTGSNDQQRGVVRIGEL
                     LDSSKIDSRTFLNLILNPGEAATDCDPFILSTLHGSKGLEWDNVILIGLNEQEFPGGK
                     PDDVYSVRTSMNTPPAEEEIEEERRLFYVGITRTKQQLNLVVPLDEGLARWLKNRWDS
                     TPKKSPIATRFVYEAGWTACAVTSDAIYNSTVEKQKADFSKFHQWYLRDLQRLKV"
     gene            complement(14075..14329)
                     /locus_tag="RHV13_002425"
     CDS             complement(14075..14329)
                     /locus_tag="RHV13_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1241951.1"
                     /translation="MLALGVSYPPKSGWIERLIGTEVSDEQYERFLGHSTSKQAEQIL
                     RGEQPAKGLQYAKRAKKLASERKATIDLDNEHLSEIEKYR"
     gene            complement(14455..14689)
                     /locus_tag="RHV13_002426"
                     /pseudo
     CDS             complement(14455..14689)
                     /locus_tag="RHV13_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016162084.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
     gene            15315..15656
                     /locus_tag="RHV13_002427"
     CDS             15315..15656
                     /locus_tag="RHV13_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1241952.1"
                     /translation="MAKVQGLFVGYRKFAVDRDWLRQQEEQRYRDRQRQFDEWSRKWV
                     TVTRLKETRLWTDGAIRRWLGEPQQQGKYKVFPVEAVLAAEKLNEFRLWLKPRLEKKR
                     AQHHHFLIPFL"
     gene            15748..15939
                     /locus_tag="RHV13_002428"
     CDS             15748..15939
                     /locus_tag="RHV13_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1241953.1"
                     /translation="MEGIETLSLQLDENETMALAQLVKRLSWSDLRGCAVSDEEAWVM
                     KSAIEKLQQALREEGYAPR"
     gene            complement(16180..16635)
                     /locus_tag="RHV13_002429"
     CDS             complement(16180..16635)
                     /locus_tag="RHV13_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000374058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1241954.1"
                     /translation="MDRIKYLKWIAEESPSTAQQLVAWLNRARHYTPDMKEHQAGVQI
                     QEKGIVVGLRQSTNRYHGDCLTIHVVRLPEEIQNKGWFKSFLKLCCESNPWCDVVIED
                     VKNPYLLSFCKKLNFTVLDEFYPNTYIVNTDAIMSLPIPPLGRYETYLY"
     gene            complement(16726..17967)
                     /locus_tag="RHV13_002430"
     CDS             complement(16726..17967)
                     /locus_tag="RHV13_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDV1241955.1"
                     /translation="MNLQSGQNIPLQQSAIRLNLQYPTKSGFKGEPDTCLFLLNAQGK
                     VSGDSDFIFYNNLSSPEGAVKLVTGSQQSSIEIALDRVPANISKIAITVVIDGEDTIS
                     GLSSLSMQAQGIAEFQAETQGRSEKAIILGEVYRHNGAWKLRALGQGFNGGLEPLAIS
                     YGVDVAQPAPQPAKPARISLEKKLETRSPRLVSLAKKASVSLTKNKLDTLEAAVAFVL
                     DASGSMSGQFSKGNVQSVLDRIAVLAAQFDDDGEMDVWGFGEKHKKYPNVTLDNLDTY
                     IQSIRGAGKRSAWENLPGLGGTNNEPPVMEEIVDYFKDSKIPVYVVFITDGGISKTRA
                     IKDAIRRSANYPIFWKFVGLGGSSYGILKNLDDFTDRRVDNTHFFAMDDFGSISDEKL
                     YDNLLEEFRPWIDETKRLGIL"
     gene            complement(18395..18970)
                     /locus_tag="RHV13_002431"
     CDS             complement(18395..18970)
                     /locus_tag="RHV13_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDV1241956.1"
                     /translation="MAVSLVKGGNVSLTKEAPTMNVAMVGLGWDARVTDGQGFDLDAS
                     VFAVGEDGKVLSDAHFIFFNNKTSPDGAVEHQGDNRTGEGDGDDEQVKIDLTKVSADI
                     KKLVFAVTIYDAEARKQNFGMVSNSFMRVYNNDNGTEIARFDLSEDASTETAMVFGEL
                     YRHGAEWKFKAVGQGFAGGLAALASQHGVNI"
     gene            complement(19039..19617)
                     /gene="terD"
                     /locus_tag="RHV13_002432"
     CDS             complement(19039..19617)
                     /gene="terD"
                     /locus_tag="RHV13_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerD"
                     /protein_id="HDV1241957.1"
                     /translation="MSVSLSKGGNVSLSKAAPSMKNVLVGLGWDARSTDGQDFDLDAS
                     AFLLASNGKVRGDSDFIFYNNLTSSDGSVTHTGDNRTGEGDGDDESLKIKLDAVPSEV
                     DKIIFVVTIHDAQARRQSFGQVSGAFIRLVNDDNQTEVARYDLTEDASTETAMLFGEL
                     YRHNGEWKFRAVGQGYAGGLASVCAQYGINAS"
     gene            complement(19666..20706)
                     /gene="terC"
                     /locus_tag="RHV13_002433"
     CDS             complement(19666..20706)
                     /gene="terC"
                     /locus_tag="RHV13_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerC"
                     /protein_id="HDV1241958.1"
                     /translation="MVSTHIGFPTETVIVFIALSVGAIFIDLFMHRDDKPISLKSAAL
                     WSVFWVVVAMAFAGFLYIHHGAEVASLFVTGYALEKVLSVDNLFVMMAIFSWFAVPDR
                     YRHRVLYWGIIGAIVFRGIFVAIGTSLLSLGPYVEVVFAIIVAWTAVMMLKSGDDDDE
                     IEDYSQHLAYRMVKRFFPIWPKLRGHAFLLNQKEVDAELAKPENSDVTIGRGKKAALY
                     ATPLFLCVAVVELSDVMFAFDSVPAIIAVSREPLIVYSAMMFAILGLRTLYFVLEALK
                     QYLVHLEKAVIVLLFFIAAKLGLNATDHIWHHGYSIAATTSLYVVLGVLALGILASVM
                     FPGKPESEEKGS"
     gene            complement(20729..21184)
                     /locus_tag="RHV13_002434"
     CDS             complement(20729..21184)
                     /locus_tag="RHV13_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HDV1241959.1"
                     /translation="MSFFDKVKGAINSGRDELTRQVGRFKNKKFMQGTVAVCARIAVS
                     SDGVSSEEKQKMMGFLRSSEELKVFDTNEVIEFFNKLVSSFDFDVEIGKGETMKYILA
                     LKDQPEAAQLALRVGIAVAKSDGNFDQDEKLASREIAIALGFDPAEFGL"
     gene            complement(21207..22364)
                     /locus_tag="RHV13_002435"
     CDS             complement(21207..22364)
                     /locus_tag="RHV13_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDV1241960.1"
                     /translation="MNLTPGGNAPVPAQELRVRITSGGQVDASAFRLYADGKVQGDAD
                     MVFYGQPRNDDGTVSLVSEGQYSTFTVALNRLKPDVQKIAFTVTCDGGQTVSGLRNLS
                     IDVEQGATGLVSGSVELSGRQEAALILGEFYRRNNDWKFRFVAQGFNGGLKPLAEHFG
                     VNIADEPAPAAPTPPVVTPPPVETKISLSKVSLTKEKPAISLTKRDNFGEIRINLNWH
                     RGSGKSGFAGMFGSKGIDLDLGAFVELQDGYKSVIQALGNAFGDYRDEPYVQLKGDDR
                     TGDVSDGEWLHINGREWKHIREVLIYAFIYEGVPSWDKTDGVVTIHVPDQPPIETRLT
                     EGENRRTLCAIARLVNENGAIKVERINQYFKGQDEMDRAFGWGFRWSAGSK"
     gene            complement(22364..22945)
                     /locus_tag="RHV13_002436"
     CDS             complement(22364..22945)
                     /locus_tag="RHV13_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance-associated protein TerZ"
                     /protein_id="HDV1241961.1"
                     /translation="MVSLVKNQTVSLSKESSALSQLHFGLGWDPVKKKGLLGGLFGGN
                     DSIDLDAGCVLMDSTGKTIDTIWFRKLESTCGAVVHSGDNLTGEGDGDDEVINVNLSR
                     LPANVEYLAFTVNSFRGQSFNDVENAFCRVVDQTGKELARYKLTEQGSHTGIVISSLR
                     RNNGNWDFTALGHACRGRTIDDMHSDIVSAVIR"
BASE COUNT         5679 a         6068 c         5863 g         5522 t
ORIGIN      
        1 gccttcaacc aaggagacat cgtgcagggg caccgcatcg gctacgtccg ggtcagcagc
       61 tttgaccaga acccggaacg ccagctggaa caaacccagg tgagcaaggt gttcaccgac
      121 aaggcatcgg gcaaggacac ccagcgcccc cagctcgaag cgctgctgag cttcgtccgc
      181 gaaggcgata cagtggtggt gcacagcatg gatcggctgg cccgcaacct cgatgacctg
      241 cgtcgcttgg tacagaagct gactcagcgc ggcgtgcgca tcgagttcct gaaggagggc
      301 ctggtgttca ctggcgagga ctcgccgatg gccaacctga tgctgtcggt gatgggggcc
      361 ttcgctgagt tcgagcgcgc cctgatccgc gagcggcagc gtgagggcat cgccttggcc
      421 aagcagcgtg gcgcgtaccg gggccgcaag aaagccctgt ccgatgagca ggctgctacc
      481 ctgcggcagc gagcgacggc cggcgagccc aaggcgcagc ttgcccgcga gttcaacatc
      541 agccgggaaa ccctctacca gtacctccgc acggacgact gacacatgcc gcgtcgcttg
      601 atcctctcgg ccacggagcg ggacaccctg cttgcgctgc cggaaagcca ggatgacctg
      661 atccgctact acaccttcaa cgactccgac ctgtcgctga tccgccagcg acgcggcgac
      721 gccaaccgcc tcggcttcgc cgtgcagctc tgcctgctgc gctaccccgg ttacgcgctg
      781 ggaaccgaca gcgagctgcc cgagccggtc atcctgtggg tggcgaagca agtccaggcc
      841 gagccggcga gctgggcaaa gtacggcgag cgcgacgtga cccgtcgcga gcatgcccag
      901 gaactgcgca cctacctgca actggccccg ttcggcctgt ccgacttccg cgccctggtg
      961 cgcgagctaa ccgagctggc ccagcagacc gacaaaggct tgctgctggc cggtcaggcc
     1021 ctggagagcc tacggcagaa acgacgcatc ctgccggcgc tgagcgtgat tgaccgggcc
     1081 tgctcggaag ccattgcgcg agccaatcgg cgggtctacc gcgccctggt cgaaccactc
     1141 acggactcgc atcgggccaa gctggacgag ctgttgaagc tcaaggccgg cagcagcatc
     1201 acctggttga cctggctgcg ccaggcaccg ctgaaaccca actctcggca catgctggaa
     1261 cacatcgagc ggctgaagac atttcagttg gtggacttgc ccgaaggcct gggccggcac
     1321 atccaccaga accgcctgct caagctggcc cgcgagggtg ggcagatgac gcccaaagac
     1381 ctcggtaagt tcgagccgca gcgccgctac gcgaccctgg ccgccgtggt gctggagagc
     1441 accgcgaccg tgatcgatga gctggtcgat ctgcatgacc gcatcctggt caagctgttc
     1501 agcggcgcga agcacaagca tcagcagcag ttccagaagc agggcaaggc gatcaacgac
     1561 aaggtgcgcc tgtactccag gatcggccag gcgctgctgg aagcgaagga aagcggcagc
     1621 gacccctatg ccgccatcga ggcggtgatt ccctgggacg agttcaccga gagcgtcagc
     1681 gaggccgagc tgctggcccg gccggaaggc ttcgaccacc tgcacctggt cggcgagaac
     1741 ttcgccaccc tgcgccgtta cacgccggcc ttgctggagg tgctggaact gcgcgccgcg
     1801 ccggccgcgc aaggcgtgct ggcagccgtg cagaccctgc gtgagatgaa cgccgacaac
     1861 ctgcgcaagg tgccggccga tgcacccacg gccttcatca agccgcgctg gaagccgctg
     1921 gtgatcaccc cggaaggcct cgaccggaaa ttctacgaaa tctgcgccct gtccgagctg
     1981 aagaacgccc tgcgctccgg cgacatctgg gtcaagggct cgcggcagtt ccgcgacttc
     2041 gacgactacc tgctgccggc cgagaagttc gccgcactca agcgcgagca ggccctgccc
     2101 ctggcgatca acccgaacag cgaccagtac ctggaagagc gtttgcagct gctggacgag
     2161 cagttggcca ccgtcacccg cctggccaag gacaacgagc tgcccgatgc catcctcacc
     2221 gagtcagggc tgaaaatcac cccgctggat gcggcggtgc cggatcgggc gcaggcgctg
     2281 atcgaccaaa ccagccagtt actgccgcgc atcaagatca ccgaactgct gatggacgtg
     2341 gacgactgga cgggcttcag ccgccacttc acccacttga aggacggggc cgaggccaaa
     2401 gacaggacgt tgctgctgtc cgcaatcctc ggtgatgcga tcaacctcgg gctgaccaag
     2461 atggccgagt cgagccccgg cctgacctac gccaagctgt cctggctgca agcctggcac
     2521 atccgcgacg aaacctattc ggcggccttg gccgagctgg tcaaccacca gtatcgccac
     2581 gcctttgccg cccactgggg cgacggcacg acctcatcct ccgatggcca gcgcttccgc
     2641 gcgggtggcc ggggcgagag caccgggcac gtcaacccga agtacggtag cgagccggga
     2701 cggctgttct atacccatat ctccgaccag tacgcgccgt tcagcacccg cgtggtgaat
     2761 gtcggcgtcc gcgattccac ctatgtgctc gacggcctgc tgtaccacga gtccgacctg
     2821 cggatcgagg agcactacac cgacacggcc ggcttcaccg atcacgtctt tgccctgatg
     2881 cacctgctag gcttccgctt cgcgccgcgc atccgcgacc tcggcgaaac caagctgtac
     2941 gtgccgcagg gcgtgcaagc ctacccgacg ttgcgcccgc tgatcggcgg caccctgaac
     3001 atcaagcacg tgcgtgccca ctgggacgac atcctgcgcc tggccagctc gatcaagcag
     3061 ggcaccgtca ccgcctcgct gatgctgcgc aagctcggca gctacccgcg ccagaacgga
     3121 ctggccgtgg ccctgcgcga gctgggccgg atcgagcgca cgctgttcat cctggactgg
     3181 ctgcaaagtg ttgaactgcg ccgccgcgtg catgccggcc tgaacaaagg tgaggcgcgc
     3241 aactcgctgg ccagggcggt gttcttcaac cgccttgggg aaatcaggga tcggagcttc
     3301 gagcagcagc gctaccgggc cagcggcctc aacctggtga cggcggctat cgtgctgtgg
     3361 aacacggtgt acctggaacg cgccacccag gggttggtcg aggccggcaa gccggtggac
     3421 ggcgagctgc tgcaattcct gtcgccgctg ggctgggagc acatcaacct aaccggcgat
     3481 tacgtctggc ggcagagccg cagactggaa gacgggaagt ttcggccctt acggatgccc
     3541 ggaaaacctt agcgtacgat tttttccgaa ttctgcgggc tccccacgaa tggctattac
     3601 ctgttacttc agggcggcaa aaaggtaaaa aagtgcatat cccacgacaa tgatgagcaa
     3661 taccctggag acaatgcagg gttggcaatt ttgtccatcc agtccgcagt aggcaccgat
     3721 ttttggagtg ttttcgtctt tcatagttta atccttgtca ggtatccctc cctgtttatt
     3781 cggcagggtg tccctgcgtt aacggggaag ttgtcatacg tccgtaagtg taaccaagga
     3841 cagcccccca aatcagatgc agtattagtg tcatcaccgg cgccataata cccagtgtta
     3901 tgccgaagaa tccggcaccg gccatgggca tcggtattaa catcatcaac aaccaggcca
     3961 gtacggagaa cattagtcct ttctttgtgg cactttgccc cggcaaccgg ttatacaaca
     4021 gcgcaaaaag cagcccccag agtaccgtgc cgatcatgaa atgagcgagc caaccaatgt
     4081 tcagccccgc agaaataccc atcatggagt gagccatacc gctgagcatg ccgacggggt
     4141 tgagttcggg catgatgccc atagaacttt tcatcaccat cagagcagac agcacgatgg
     4201 ttgcgatgaa gcctgctgcc atggatttca gataacgatt catatttttc ctctatcttt
     4261 agggaagtgg ttactccgga tttaagggct ggacgatgtc gcgccatacc attacccaat
     4321 aagagggggg aacaagggaa aaggattcgg ggagaagtaa aaaaaatgaa aattgaagtg
     4381 atggtgatca agaattatct tcttgtgttc cgcttggcgg gcgggaaaac gcactgccac
     4441 aaggtgctgc gtctggcaag ttctataaag tagggaacgg tatcagcatc actgatgatg
     4501 aaaaagctgg caagtcatcc gaaacagaat ggtctggcaa aggcgctgag agagattagg
     4561 cgtatcgaaa ggtgactggt tcagggatcc ttcactgcgt cgacgtgcac aggcggggct
     4621 gaataaaggt gaggcgcgca atgcccttgc ccgagcgttt ttttacaccg gctgggcgaa
     4681 atcagagatc ggggggtgga gattcattac tatcgagcca gcagtatgac gctgctgatt
     4741 gcggcgatct cactgtggaa tacggtatat atagaaagag cgataaattc tttgagacgg
     4801 aaagcgatac cgattaatga gcaactgatt acccatttgt ccccgctggg atgggaacat
     4861 atcaacctga gaggcgatta cgtctggcga aaaaaaactc aaactgggac agagtaaata
     4921 tcgcgcctta cgctcagtgg atagtagcct atacaataaa caggcttagc gtaggatatt
     4981 taatgagatg gtcactccct ccttcccagt actatgctga ggacaggctt tcattcggag
     5041 aaccatcatg gaaaacattg cgcttattgg tatcgatctg ggtaagaact ctttccatat
     5101 tcattgtcag gatcatcgtg ggaaggccgt ttaccgtaaa aaattcaccc gaccaaagct
     5161 aatcgaattt ctggcgacat gcccggcaac aaccatcgcg atggaagcct gtggcggttc
     5221 tcactttatg gcacgcaagc tggaagagtt agggcatttt ccaaagctga tatcaccgca
     5281 atttgtccgc ccattcgtta aaagcaacaa aaatgacttc gttgatgctg aagctatctg
     5341 tgaagcagca tcacgtccat ctatgcgttt cgtgcagccc agaaccgaat ctcagcaggc
     5401 aatgcgagct ctgcatcgtg tccgtgaatc cctggttcag gataaggtga aaacaactaa
     5461 tcagatgcat gcttttctgc tggaatttgg tatcagcgtt ccgcgaggtg ctgccgttat
     5521 tagtcgactg agtacccttc ttgaggacag tagtttgcct ctttatctca gccagttact
     5581 gctgaaatta caacagcatt atcactatct tgttgagcag attaaagatc tggaatctca
     5641 gttgaaacga aagttggacg aagatgaggt tggacagcgc ttgctgagta ttccctgcgt
     5701 tggaacgctg actgccagta ctatttcaac tgagattggc gacgggaagc agtacgccag
     5761 cagccgtgac tttgcggcgg caacagggct ggtaccccga cagtacagca cgggaggtcg
     5821 gacgacattg ttagggatta gcaagcgggg caacaaaaag atccgaactt tgttggttca
     5881 gtgtgccagg gtattcatac aaaaactgga acaccagtct ggcaagttgg ccgactgggt
     5941 cagggagttg ttgtgtcgga aaagcaactt tgtcgtcacc tgtgctctgg caaacaagct
     6001 ggccagaata gcctgggcac tgacggcgcg acagcaaact tacgaagcat aaaggcagaa
     6061 atacaccagt ttaaacaatc attcatctgg ttttgcgaat actgatattg atgatactaa
     6121 cggcccaccg gcctgttgag gaacctgtaa aacggaaagg ctcattgaag ccgtatattt
     6181 tctggaggtt catcaggcgc ggaactcatc gaggcgcggg aataaaatcc cattcagacg
     6241 ccggatagat tcaagcaagc caacttgtcg tcaaaatcgg tgttgcaaaa acgggagtga
     6301 ccatagattc cgttttccaa gcggacccct acttgactgg caagtttcct gctctgatgg
     6361 tcaatgcctc gatgctgtct atattgttct gaagccgttc cagttctgcc ttttcaatac
     6421 cggcccaaag ctttaagtgt cgctgataaa gggaccactt gagtgagtga aaaccattcc
     6481 aggccaaccc catccaggag gcgtaattcg cattgagaat attgccggtg atatacattt
     6541 tacctgcgtt aacagcactg gaagttccat aacccagggc aagcatcatc cggaagcgtg
     6601 gatttagccg caacggcatg gtatccagta aggtctcccc aaaagctcct tttcccagcg
     6661 atacctgttt cgccacatag aaaacccgca tcaaaacttc cattatgctc atcggaatac
     6721 ttgtcacgat aaactgacga aagtcatacc cgttgacata catgtactct atttgtttgc
     6781 cgaagttaga actccccaca gggatcccct caagcatacg aagtagcccc ataaaaggag
     6841 ccggtaaccc catgccacgg tttccttttg ctgagggggc attaacatcc gatgccaggt
     6901 gtccaaacat tctggcgatg agcctgaaaa tattggtttc gtctgtaacg cctttactgg
     6961 aaggataaac cacgatctgt ccattctgaa ccaccgtaca ggtcccgttc agcatatcct
     7021 ttataccgaa aataatgccc agtacgggat cgtgtgacaa agctcttaag cggtgatttg
     7081 tgggagacaa aacgccaccg gccgcaccca ctaaatcgct gctgatcgaa ctgtctggtg
     7141 cccctatagg gaacaaatct gagagttttg tactcaggtc ctccggcaaa atggcattga
     7201 aggctttctg cacctgttta ttgaaaatac catccacttc tgccgtaaag ctcaccgtag
     7261 gttttggcgg ggctctgacg caaagtaaat ccagcatgga tgctatgagc ccgcatccgc
     7321 aggcaatagc ataatcccag gcgtcgagtg cgtagcgatc attaaattct ttaatatgat
     7381 tctggatccg ggcatctaca acagccaagt catccgtgga gagtattgtg gccagtccaa
     7441 tatcaccgca tacacgctgg ttagcctcgc tatatatagt cttgagacca gtagaatgca
     7501 tggcagtatc tgaaggggtc atttctcgca gatgatgttc tttaagcgcc agatgctccg
     7561 cttcatcaag ttcagtgccg ctgattagcc cggtcaactg tgccttaagg ctctctatat
     7621 cactgttaat tgtctcgtga gcgttcgcga tatcctgacc acggagctga atataacgga
     7681 tggccctgaa ctcatcatca taaggagact gactcatgtg tttacctcac cgcgaccagg
     7741 ctaccttttg gagctttctt agtaagcatg tcgattgttg ccttgatgcc agcaatttct
     7801 gccttaaaat attccgcatt cgacatcaac ctttcctgaa cttcgtagag cttttggatg
     7861 gcctgactaa gggcggctgt cagtttcgca ttttttttgt gtttcactat cgcgtacccg
     7921 ccaatcccta acacagctac aggtgcggcc aataccgcaa tgcctgcaac cataccgcca
     7981 ccgacgatag accctgctgt agcgagcccg gaggttatgc ctgctgctga cactcccgtt
     8041 gtaccaagcg tatagagagc accgaaggaa gccgctgcgc ctacgcctga ccccgccaaa
     8101 gctgctgcgc cctcagtaat acccgggaac ttattttcaa gcgcctgaag agcacttgta
     8161 acttcatcca ttgccttctt gcgaattttt tcgagctctt cagtttcggt agcgtccatt
     8221 acaggcctcg tattcataac ttgctgtgtg ataggtgaaa aagagcacca taaaagtgat
     8281 gaagaaatca tgacttacta ttgtggcact ttcgagttca tgtttgtcag tttcaatgct
     8341 gttgctgtta gtaagggcag acgccatatc gggcagaccc aaaataacta tcgtgccagc
     8401 gtcattttgg aactgacttt gctaaaaatc agtagcggac tgaacagaca tgtgtggatt
     8461 tgaatctggg caggttccag acattattcc aaccagactt ccaatgacaa aatgctcgtc
     8521 attctcggag acgacatagg gtccaccaga gcaaccatcg gtgttgaaat gtgctattac
     8581 aaagcgagtt gtgagatgtt ggggagaatg atttgagcaa tttaaaggcc aaatgcctga
     8641 aacttcacca gggaatgtcg aaattgttgt ctcattcatt ggatatccga cacccaatgt
     8701 acccatgcca gctgcgggag ccaggacctt aaagtaacta cttacaggct gttgtattcc
     8761 agctggtaat acgatttctg cactgtcaac aaacggaatg ccgtctttaa agtctgccct
     8821 gtcattagcc tcattgagaa tgaaatcgac tagaacaggc tcttcgttca ccagtccttg
     8881 atggttaaga aatttgacgt aaagtttaag tgtattgctg acgggataaa tttttccaag
     8941 agcattggta acaacatggg ctgcggtgag aatcttttgc tgctgaccat tatcgaaacc
     9001 aaacgcagtt gcttcgagat ggctactaaa atgaggatca ttgcttacat agattttaca
     9061 aacgtagttt tcaaacatta tttcctcctt ttgccggtac ctaatcagtt acacggctgc
     9121 atgttataaa caactaaact cgcttcctga tatttgagtt tgtatgtttt gccaggcacc
     9181 agctcacctc tgaaacacgg ttagtctcct ccatgacatg gttatggcga tggtcagaaa
     9241 ctgttatcga cgaaaggctg gaattccttt aatggcatta tccatttcaa gtcgtatgtc
     9301 ctgaacggga gcgtatattt ttatgattcc agatcttaac cctgaaacat tccccgtatc
     9361 aaaactgacc cgttacagat aagtcaccac cggtgctgtc gattaattta gtgaattgca
     9421 tgtcctcatc ccataaaggg tagggcctac ccccggcatc gagcaaacct gaccaccctg
     9481 cccttattaa gaccccacat gcggcaatgg tgccctgtcc acgccgcccg cgaactttga
     9541 ggaaatgggg agaaaataaa aagaaaacaa aaagttatcg gctgaagtac cggcgcagat
     9601 cgggaatcag gcgaagcctg acagcacgtt agggcctggc agggggcttg agggatgagg
     9661 acatgcaatt gtgataacct tcccgccacg gggtcggtta aattcgtatc ctgccggtgc
     9721 aattccaacg gccgcgttac cagttcgccg ggtagcatgg ccagcaaagc caggcatcga
     9781 gcacccgggt ttcactgacg gccgctgcat agacgccggt atccgggaag aaataaaccg
     9841 gggtggcgtt gttcagctgg tcgccttcat cggccagagc gccgatcacc tcatgaggct
     9901 taagcagcat atccgttaaa tattcatccg ggtggatggc attcagcgat atggccatct
     9961 ccagaaattc atccagggta tggcgggtac gatacccccg gaaaacctcg tatgaaggcg
    10021 tgacgcattc accatccagg gaacaggcat cgtaaccggc cagctgctct tcggtgagtc
    10081 cggaggcatg ctttgccaca agattcgtaa attgctcttt atcgtccatc acaatcctta
    10141 cgctgtaggg gcctgtttgt gttatcactg cttccggttt ttacttcgca caacccggca
    10201 ggtatccggt cgcccgggcg tcctttgcgg tcttgttcgc ccttcgcctg aattacgtca
    10261 aatccctttc actacccggc cgcagggtga cacctcccct tttctccgga agcgaaagag
    10321 gaagaacagt ttaacagccc ggatcctcaa taattttcgc gtaacggact aaataaccgg
    10381 tttatgcagc ttctgataac cacagaccgc gatagaataa aacgtctggt gaggaaccgg
    10441 agtgttatgt aagcgggggg tttaactggg tatctgtgta gtcctattta gccagatatc
    10501 ccgaataaaa acctcaacga ttaggtaaac gaaatggatg gattctggtc gatgttctgt
    10561 ttgtttgtcg tggtagtttc actgcataaa gccctttctg tagcagcagc acgagcagac
    10621 gcagcaaaat taaatatgct tctcagaatg agaaagttta aaaagtagct ttcaagacat
    10681 ctgagaattc ttttaaatcc tcagaggaag ttatatgttt aggctatttg caaactaagg
    10741 ttggaattcc atcattctag ccttagatct ttgatcaagt gaagttgctc acttggttgg
    10801 attttaccgt ttcctgacgg cttttcagaa gcgagggctg gttggaatgc cttccagcta
    10861 gaagtcttgc agatgctctc cgggcattaa ctcattgtcg aatctgcacc agtatcacta
    10921 ggttgttgag cgggcgatta gctttttcac tgagccgctg tacactcgac tgcttcaaga
    10981 agcactactt accatttgcc tgccgtaagg tctgttctta gctggatttt tttgggagga
    11041 tacgcagtca tgacaactca atccagctca atacaatacg cttacgcgct agacggcgag
    11101 ggcaccctca cgcacattgg cgctgccctg cgctcacata cttacacctg cccaggttgt
    11161 aagagccccc tgaccccggt aatgggcgag ttcaacgcta agcactttcg ccattcggaa
    11221 gaatgctgtg cccttgaaac ctatcttcac aagtgcggca aagaagcatt cttctatcgc
    11281 taccaacaag ctctcagccg tgaaatgcct attagccttg agctggagcg ccgggttgcc
    11341 tgtaatggtt ctcgtttagc cttggttaga gacgaagcac gtcagtgcgt aaaatccgtg
    11401 cctgcacgct acaacttgac tcaatttttc gatcaggcag agctggaaaa acacgataag
    11461 gtcacagggc tgcgaccaga cgtgatgttg tatgatacga ctggcgaaag gcgttgctac
    11521 gtagagattt gtgtcacaca cccctgctct caagacaaga tcgaagccgg tatcccgatt
    11581 cttgagttta aggtccaatc tgcctctgat attcagatgt tgcttaccgg ggcctactcg
    11641 atcaaggaga agatcctgag agtattcaac tggcttcccc cgtttcaatc tgtggacacc
    11701 tgtagcggcg tctgctcagt aggtaacgtg gatatgtctg tctggagttt aagtggttcc
    11761 ggccgactca acgaacaaac gatgccgctc gctgaagtgg atttaacaat caattctgac
    11821 gtgaacacat ggcctagatc gttaggggcc gctgagctgg ctgataactt acgagctttt
    11881 atccgtcacg ccgatcctca ttcactattt cccaactgca ttatgtgcga gcaagcaggc
    11941 cgatgggagg atggctatct gcaatgtcac agtaaggcaa agatcgttcc ctacaccgag
    12001 gcccgtcagt gcgccaatta caaggttaag gcatgaccaa aagactgact tgggagcaga
    12061 aaagtattgt cagccatgac accggacatg cgttggtaaa agccgtacct ggtagcggga
    12121 aaacaaccac acttgtaaag cgcgttgagc ggctggtcaa gacgggaact gatcctcgct
    12181 ccattctgat cctgatgtac aacaagtcgg cgcaggtgag cttcacggag aacttgaaga
    12241 cggcactgat gtcgagcgtg attccagaaa tccgcacgtt ccacagcttg gccttaaaga
    12301 ttgttggtta cggagaacgc caacagataa tcaagaagaa agacctcatt actccaagcg
    12361 actaccgcta tgagcaactg gtaaagcaag cctaccgcta tggctttgac catgaggcga
    12421 attatattga ccccaatgaa attgaaaact tcgagctgtt tattgctcgc tgccgggcgg
    12481 cggccgtaac tcctgttgat gctgccaatg atccaacgtt cagcaacatc aaacgggagt
    12541 ttattcacgc ctatggccgc tattgtgagc tgctggagga aaacagtctg cgaacctttg
    12601 atgactgttt gattgaggct gtcgcactgt tgcgtaatga cagcagcctt ggtgcccact
    12661 tcaagcacat cattgttgat gaatatcagg acgttaacct gatacagcat gacatgaccc
    12721 gcttgctatc taagtcggat acgtcggtta tggctgttgg tgatgttaac cagtgtattt
    12781 atgagtggcg tggtgctcga ccagacttta tcggcggact gtttgaaagg cactacccga
    12841 atacgaaggt gtttcaactg tcatgcacgt tcagatttgg ccatgagctt tccttgatgg
    12901 ctaactcggt aattcggcgc aactctacaa agctgaccaa gctttgtgtc agtcatccta
    12961 gcacccccaa aacagaggtc agattgcact ttgataactg cctatccaag gtgctatcaa
    13021 acctctcggt gagcagtgga acacaagcca tattgtcccg cactaaggcg aaccttgctg
    13081 aggcggaaat tgctttacgc ctgtgtgggc taccttatcg ctacctcaat ggttcaagcg
    13141 cattacacac ccgcaccgaa atcgggatac tggtagttgg cgtcttgctg tctgtgtacg
    13201 gcgatctgcg gctactggaa aaccatccca acaaacaagc gattgtttac ggctttctga
    13261 aggaggctgg ttttagatgg cagaaagggc aattcaaagc ggcgctcagt ggattgatgg
    13321 ctccgcacgc cgacctatgg tctgctctag gtcagttatt cgagggcgca caataccaaa
    13381 aagatcggct gggcaggctc gccactatct gccagaaaga cggagaggaa acgcctgcta
    13441 tcgatgtgtt acgacgcctg agtatggagg gtttcattga tagtgttggc tctgagggtg
    13501 taactcgcac tggatcgaac gaccagcaac gaggggtcgt gaggatcggg gagctacttg
    13561 actccagtaa gatcgattcc cgcacgtttt taaaccttat cttgaatcca ggcgaggctg
    13621 ctactgactg cgatcccttc atcctttcca cactgcatgg ttccaagggg ctggagtggg
    13681 ataacgtaat actgatcggg ctgaatgagc aggaatttcc tggcggcaaa cccgatgatg
    13741 tttacagcgt tcgcacatcg atgaacaccc ctccggccga agaggaaatc gaggaggaac
    13801 gtcggctgtt ttatgtcggt atcactcgca caaaacagca gctaaatctg gtggtccctc
    13861 ttgatgaagg gcttgcgcgg tggctcaaaa accgctggga tagcaccccg aagaagtcac
    13921 cgatagctac tcgcttcgtc tatgaggctg gctggactgc ttgtgcggtt accagtgatg
    13981 cgatctataa cagcacggta gagaaacaga aggctgactt cagcaagttt caccaatggt
    14041 atctaagaga tcttcagcgg ctaaaagtct agggctaccg atacttttcg atttcagaca
    14101 ggtgctcgtt atccagatca attgtggctt ttctttcaga agcgagcttc ttcgctcgct
    14161 ttgcatactg aagccccttg gctggctgct ctccgcgtag gatctgttca gcctgcttgc
    14221 tcgtgctgtg ccccagaaag cgttcgtact gctcgtcaga tacctcagtg ccgatcagtc
    14281 gttcgatcca gccgcttttc ggcgggtaac ttacgcccag cgcaagcaac tgccttttct
    14341 gtacccacta tttccggcgc ggccggcttc aatcaaggct ttggttagaa ctacagccat
    14401 ttggggactc tcatacacaa acctgaacca tgcgggtctg attggcggtc aagatagcag
    14461 cgcatcgaaa tcaacactga actctgcttc taagaactca atcgtggctg gttggctatc
    14521 gagcgatgct tcccgtgtag actccagtgc gcactccaat cgacgcagca gagtaaacgg
    14581 caaaatgatc ttgccgaagt cggtatgctt gaaatccccc ccacaggtct tccgcgttct
    14641 tccaaatgaa atcagcttgc gaagcagccg aacctacgaa ctctgccatc ttggcctcca
    14701 accaactttt gcacgaaacg gcacatcttc caaaagaggg ttgtcaacca agttttgcac
    14761 aaaacggact ttgatccatt tctaactaaa atctacaatc caacctcaga ttgcaaatag
    14821 cctatgttta ttacagatgg tatggttcgt gtttttgttt attcgatggc aactgtactc
    14881 tttctgccgc ttggcttgtt gaaaagcacg tggtggtttt caccattgtt tgcgatagct
    14941 gctattgatt acttaatgtt tctgcgtcga caaggctagt tgctgaacct catacctcag
    15001 agcaatctgt atgtttttta caccactggt taaaaggtca ggctacgttc ctgacgtttt
    15061 ttaaaaaaca gttttatgtt tgatgcgtta ctggtctgtc tggtaaccca gtggtacatt
    15121 accattatta aggctggctg gcggtggtgc atcatgctta aacgaccctc tctgagcgta
    15181 ctttccgaca gtcctgtgca ccgcaccact tacccggcag gcctatgtct tggttattct
    15241 tcgttgtacc tttttcttca gctttatccc tgacatgtct gtctgaacag ttctaagcaa
    15301 accggaatgt tgtcatggcc aaagtacaag ggctctttgt tggataccga aaattcgctg
    15361 ttgatcgcga ctggcttcgt cagcaggaag aacaacgtta tcgggatcgc cagcgacaat
    15421 ttgatgaatg gtccaggaaa tgggtcactg taaccaggct caaagagact cgtttatgga
    15481 ctgacggagc catcaggcga tggttgggtg aacctcagca gcagggtaaa tataaagttt
    15541 tccccgttga agcggttttg gctgctgaga agttgaatga gtttcggctc tggcttaaac
    15601 cccgactgga aaagaaacgt gcccagcacc accacttttt aattccattt ctgtaaagta
    15661 gaaacaacgg cagttccggc cgcattcaag gcttcggcat ctggtcagaa ataaagcgcc
    15721 atttttcagt ataagggagg ttagatagtg gaaggtattg agacactgag tttgcagctt
    15781 gatgagaatg aaactatggc ccttgctcaa ttagttaaac gtctgagctg gagcgatctt
    15841 cgtggctgtg ctgtgagtga cgaagaagcc tgggtaatga aaagcgcaat tgaaaaatta
    15901 caacaggcgt taagggaaga aggttatgcg cctcgatgag gctggcttcg ttctttattc
    15961 cgataacaaa tttgcttaag gactaactat gagccagcac tggtatgagg tgaaaagcaa
    16021 tcagggaatt gttcaggtga ttgttgggag aagatattaa tgtaatgcgt ttctgcatta
    16081 catcaagcag attagctttt tctggtcaaa atagacgatg tgatttttct ttagccgaag
    16141 tccatccgct ccccacgcaa ccggtaaagg ttcgcaggtt taataaagat aggtttcgta
    16201 tctccctaag ggtgggatag gtaaactcat aatggcatct gtgtttacta tgtaagtatt
    16261 cgggtaaaat tcatctaata cagtaaagtt tagtttctta caaaagctta ataaatatgg
    16321 gtttttcacg tcttctatta caacatcgca ccagggattc gattcacagc aaagcttcag
    16381 aaaagactta aaccatccct tgttttgtat ttcttccgga agccgtacca catgtatggt
    16441 cagacaatct ccatgataac gattagtact ttgtctaagc cctacaacaa tccccttttc
    16501 ttgaatctgt acacctgcct gatgctcttt catgtcgggc gtatagtgcc ttgctctgtt
    16561 taaccaggcc accagctgct gagccgtact tggtgattct tcagctatcc atttcaggta
    16621 cttgatgcgg tccatatgcg cctccctgta cgatttccaa tgaacaaagg ctctacagta
    16681 ttaagctgct gtagagctca ttcaaccaag ctcaaactgt gcgtattaaa ggatgcctaa
    16741 cctttttgtt tcatcgatcc acggtctgaa ttcttccagt agattatcat ataacttttc
    16801 atcgctaatc gaaccgaaat catccatggc aaagaagtgg gtgttatcaa cccggcggtc
    16861 agtaaagtca tccagatttt tgaggatacc gtagcttgaa ccacccaggc cgacaaactt
    16921 ccagaagatg gggtagttgg cagaacgccg gattgcatct ttaatcgccc gggtcttgct
    16981 gatcccgcca tcggtaataa atacaacata caccgggatt ttcgagtcct taaagtagtc
    17041 gactatttct tccatcacag gaggctcgtt gtttgtccct ccgaggcccg gcaggttctc
    17101 ccaggctgaa cgctttccag ccccgcgaat ggactgaata tatgtgtcca ggttgtccag
    17161 tgtgacgttt ggatacttct tatgcttctc tccaaatccc cagacatcca tttcaccgtc
    17221 gtcgtcgaac tgggcggcaa gaacggcgat gcggtccagc acagactgaa cgttaccctt
    17281 actgaactgg ccactcattg agccggatgc gtcaagtaca aatgcaaccg ctgcctcaag
    17341 ggtgtccagt ttatttttag taagactgac cgaggctttt ttagcgaggc ttacaaggcg
    17401 cggagatctg gtttccagtt tcttttccag actgatacga gcaggctttg ctggctgcgg
    17461 agctggctgt gcgacatcaa cgccatagct aatggccaga ggctcaagac caccgttgaa
    17521 accctgcccg agcgcacgaa gcttccaggc gccattgtgc cgatatactt cacccaggat
    17581 aattgctttc tcactgcggc cctgagtctc agcctggaac tcagcgattc cttgagcctg
    17641 catgctcaac gaactgagcc cactgatggt atcttcacca tcaatgacaa ctgtgattgc
    17701 aattttactg atgttcgcag gaacacgatc cagtgctatc tcaatgctgg actgctgaga
    17761 accggtaacg agctttactg ccccttcagg agaagacaga ttattgtaaa agataaagtc
    17821 agaatcgccg ctgacctttc cctgagcatt aagcaggaac aggcaggtat cgggttcgcc
    17881 tttaaagccg gatttggtag ggtactgaag attcagcctg atcgcagatt gctgaagtgg
    17941 tatgttttgt ccggattgta gattcacatt aactctcctg atttttgatt attttaaagc
    18001 gcagcgttta tataggttcc cctggcttag ttcaggacgc tggatttctg gtgaggcgta
    18061 tatatggtgt gacaccatat ttcatcacgt tgaagtttct atacctgttg aattggaatt
    18121 tccattggtc gggtatttta tcttccagaa cccgtgcctt cataagtcgc aaacgtaaca
    18181 tatatcagag gggcatgctg ttgatataag gcacggaata caggctttaa ccagcaacca
    18241 gagaaaaatc cgtttcctgc ctttaaaagg ctcacgtcag aattaacatc tctctgcttt
    18301 ctgagattcc cgaacccttt ctgccccccc ctcctgatag cggatcgcaa ccaaaaaaaa
    18361 ccccggcatt gccggggtat gattcagcgt tattttagat gttaacgccg tgctgggagg
    18421 caagagccgc caggccaccg gcaaagccct gaccgacagc tttaaacttc cactcagcgc
    18481 catgacgata cagttcaccg aagaccatag cggtttcggt tgaggcatct tcagacagat
    18541 cgaaacgggc aatttccgtg ccgttgtcgt tgttgtaaac gcgcatgaag ctgttgctca
    18601 ccatgccgaa gttttgttta cgcgcttctg catcatagat ggtaacggca aacaccagtt
    18661 ttttgatatc tgctgagact ttggtcagat cgattttgac ctgctcatcg tcgccgtcgc
    18721 cttcaccggt acggttgtcg ccctggtgct ctaccgcgcc atcagggctg gttttgttat
    18781 tgaagaaaat gaaatgcgca tctgacaaca ctttaccgtc ttcgcctact gcgaacacgg
    18841 aagcgtccag gtcaaaaccc tgaccatcgg ttacacgggc atcccagcct aggccaacca
    18901 tagcaacatt catggttggt gcttctttgg tcagagatac attgccgcct tttacgagag
    18961 aaactgccat ttttagctcc tgcaaacagt tgaatgaggg ctgcattaac agccccgggt
    19021 agaaaagtta ctttttgatc aggacgcgtt aatgccgtac tgagcacata cagatgccag
    19081 accaccagca taaccctgac ctactgcgcg gaatttccac tcaccattgt ggcgatacag
    19141 ctcgccgaac agcatggcag tctcagtgga cgcatcttcg gtcagatcgt agcgagcaac
    19201 ttcagtctgg ttatcgtcat taaccagacg aataaacgca ccggatacct gaccaaagct
    19261 ctggcgacga gcctgagcat cgtggatggt cacaacgaag atgatcttgt caacttcaga
    19321 cgggacggcg tccagtttaa ttttcagcga ttcatcatca ccatcgccct caccggtgcg
    19381 gttatcgccg gtgtgcgtta cggaaccgtc ggatgacgtc aggttgttat agaagatgaa
    19441 atctgaatcg ccgcgcactt tgccgtttga ggccagcagg aatgctgaag catccaggtc
    19501 aaagtcctga ccgtctgttg aacgcgcatc ccagccaagg cccaccagga cgtttttcat
    19561 tgacggagct gctttactta gggagacgtt cccgcctttg gaaagagaaa cactcataaa
    19621 ataacctctt cgattagtaa ttgttcaggt taacacttaa ggggattaac tccccttttc
    19681 ctcagattca ggtttgcccg ggaacatgac gcttgcgaga atgcccagcg ccagtacacc
    19741 cagtacaaca tacaggctgg ttgttgccgc gatgctgtaa ccatgatgcc agatgtgatc
    19801 ggtcgcattc aggccgagtt ttgccgcgat gaagaacagc agcacgataa cggccttctc
    19861 cagatgaacc aggtactgtt tcagtgcctc aaggacaaag tacagagtac gcaggcccag
    19921 gatagcaaac atcatggcac tatagacgat aagcggttca cgactgacgg caatgattgc
    19981 cggtaccgag tcaaacgcga acattacgtc cgagagttca accacagcca cacacaggaa
    20041 cagcggggtc gcatacagcg ccgctttttt accacggcca atggtgacat cgctgttttc
    20101 tggtttcgcc agttcagcat ccacttcctt ctggttaagc aggaaggcat gccctctgag
    20161 cttcggccag atagggaaga agcgtttaac catgcggtaa gccagatgct gggagtaatc
    20221 ctcaatttca tcatcgtcat caccgctttt aagcatcatg accgctgtcc aggcaacgat
    20281 aatagcgaag acaacttcaa catacggccc cagactcagc aggctcgtac cgatggcgac
    20341 aaagatgccc ctgaagacaa tggcaccaat gatcccccag tagagaacac ggtggcgata
    20401 acgatccgga acggcgaacc aggagaagat ggccatcatg acgaacaggt tatcgaccga
    20461 cagcactttc tccagcgcat aacccgtgac aaacagactg gcaacctcag caccgtggtg
    20521 gatgtagagg aaaccggcaa atgccatcgc aaccacaacc cagaatacgg accagagcgc
    20581 cgcactcttc agcgaaatag gcttgtcatc acggtgcata aacaggtcaa taaagatggc
    20641 accgactgaa agcgcaataa aaacaatgac cgtttcagtc gggaagccga tgtgtgtgga
    20701 aaccatattt tacccttagg gatgtggatc agaggccaaa ttcagccggg tcgaagccca
    20761 acgcgatagc gatctcgcgg gaggccagtt tctcgtcctg atcgaagtta ccgtcacttt
    20821 tcgcaacggc aataccaaca cgtaaggcca gctgagcggc ctcaggctga tctttcagcg
    20881 ccaggatgta tttcatggtt tcgcccttgc cgatttcaac atcgaaatcg aagcttgaaa
    20941 ccagtttatt gaagaactca atcacctcat tggtatcgaa gaccttcagc tcttctgaag
    21001 agcgcagaaa gcccatcatt ttctgctttt cttccgaact tacgccgtca ctcgatacgg
    21061 caatacgggc acatacagca acggtgccct gcatgaattt tttgtttttg aaacggccaa
    21121 cctggcgggt cagttcgtca cggcctgagt taatggcacc tttaactttg tcgaaaaagc
    21181 tcatacctgt ttcctttgtt gctgtgttat ttagaaccgg cgctccagcg aaatccccag
    21241 ccaaatgccc ggtccatttc gtcctggcct ttgaagtact ggttaattcg ctcgacttta
    21301 attgcgccgt tttcgtttac gagtctggca atggcgcaca atgtgcgacg gttttcacct
    21361 tcggtcaggc gggtctcaat gggcggttga tccggcacat gaatggtgac cacaccatca
    21421 gtcttgtccc agctgggaac accttcataa ataaaggcgt aaatcagcac ttcgcggata
    21481 tgcttccatt cacgtccatt gatatgcagc cactcgccgt ctgatacatc acccgtccgg
    21541 tcatcgccct tgagctgaac atacggttca tcgcgataat caccgaatgc attaccgagc
    21601 gcctgaatga ccgatttata cccgtcctga agctcaacaa aggcccccag atccaggtcg
    21661 attcctttgg agccgaacat gcctgcaaag cctgatttgc cgctgccccg gtgccagttc
    21721 aggttgatcc ggatttcacc gaagttatcc cgtttggtca ggctgattgc aggcttctct
    21781 tttgtcagcg ataccttgct caggctgatt tttgtctcaa ctggcggagg ggttacaacc
    21841 ggaggagtcg gggctgcggg agccggttcg tcggcaatat ttacgccgaa atgctctgcc
    21901 agcggtttaa gtccaccgtt gaacccctgc gcaacgaagc ggaatttcca gtcattatta
    21961 cgacggtaaa attcacccag aattaaggct gcttcctgac ggccgctcag ttcgacactg
    22021 ccgctaacca ggccggtagc cccttgctcc acgtcgatgg aaagattacg aagaccggac
    22081 accgtctgcc caccgtcaca ggtcacggtg aacgcaattt tctggacatc aggtttcagc
    22141 cggttgagcg cgacagtaaa ggttgagtac tggccttcac tgaccaggct gacggtacca
    22201 tcatcgttac gtggctgacc ataaaacacc atgtccgcat caccctgcac cttcccgtcg
    22261 gcatacaggc gaaacgccga agcatccacc tggccgcccg aggtgatccg gacccgcaac
    22321 tcctgggctg gaacgggggc attgcccccc ggcgtcaggt tcattagcga ataaccgctg
    22381 aaacgatatc ggagtgcata tcgtcaattg tgcggccgcg acaggcatgg ccaagggcgg
    22441 taaagtccca gttgccgtta ttacggcgca gcgaagaaat aacgatcccg gtatgtgagc
    22501 cctgctcagt cagcttgtag cgagccagct ctttgccggt ttgatcgaca acgcggcaga
    22561 acgcgttttc gacgtcattg aacgactgac cacggaagct gtttaccgta aacgccaggt
    22621 attcaacgtt tgcaggcagt cgggagaggt tgacattaat cacctcatcg tcaccgtcac
    22681 cttcaccggt caggttatcg ccactgtgca caacagcgcc gcaggtggat tccagtttgc
    22741 ggaaccagat ggtgtcaatt gttttaccgg tgctgtccat taatacgcag ccagcatcga
    22801 gatcgattga gtcgttgcct ccaaacaatc ctccaagaag accttttttc ttaaccggat
    22861 cccaaccgag accgaaatga agctggctta atgcagatga ttctttgctg agtgataccg
    22921 tctggttctt aactaatgaa accatgtttt attccttaaa atggtaatga ccgagacaat
    22981 caaaggtata tttagtaact gacgaagaaa atcatatcat gatgaagttc tctgacaagg
    23041 gaaattttgg tagttttctg taaaaacaca aaaagaaatc cttttcaggg tgttaatttt
    23101 ctaagtttaa tttaaatatc aatgtgttat ca
//
LOCUS       DAPGOF010000073        22933 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.073, whole genome shotgun sequence.
ACCESSION   DAPGOF010000073 DAPGOF010000000
VERSION     DAPGOF010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22933
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.073"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(6..1655)
                     /gene="actP"
                     /locus_tag="RHV13_002437"
     CDS             complement(6..1655)
                     /gene="actP"
                     /locus_tag="RHV13_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HDV1241962.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1652..1966)
                     /locus_tag="RHV13_002438"
     CDS             complement(1652..1966)
                     /locus_tag="RHV13_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HDV1241963.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFASILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2167..4125)
                     /gene="acs"
                     /locus_tag="RHV13_002439"
     CDS             complement(2167..4125)
                     /gene="acs"
                     /locus_tag="RHV13_002439"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HDV1241964.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINAPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGNIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4518..5954
                     /gene="nrfA"
                     /locus_tag="RHV13_002440"
     CDS             4518..5954
                     /gene="nrfA"
                     /locus_tag="RHV13_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HDV1241965.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            5999..6565
                     /gene="nrfB"
                     /locus_tag="RHV13_002441"
     CDS             5999..6565
                     /gene="nrfB"
                     /locus_tag="RHV13_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HDV1241966.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6562..7233
                     /gene="nrfC"
                     /locus_tag="RHV13_002442"
     CDS             6562..7233
                     /gene="nrfC"
                     /locus_tag="RHV13_002442"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HDV1241967.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7230..8186
                     /gene="nrfD"
                     /locus_tag="RHV13_002443"
     CDS             7230..8186
                     /gene="nrfD"
                     /locus_tag="RHV13_002443"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HDV1241968.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8206..9924
                     /locus_tag="RHV13_002444"
     CDS             8206..9924
                     /locus_tag="RHV13_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HDV1241969.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSTLFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRAQLPALLAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVMLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            9917..10300
                     /gene="nrfF"
                     /locus_tag="RHV13_002445"
     CDS             9917..10300
                     /gene="nrfF"
                     /locus_tag="RHV13_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HDV1241970.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10297..10893
                     /gene="nrfG"
                     /locus_tag="RHV13_002446"
     CDS             10297..10893
                     /gene="nrfG"
                     /locus_tag="RHV13_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HDV1241971.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALTLDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11235..12548
                     /gene="gltP"
                     /locus_tag="RHV13_002447"
     CDS             11235..12548
                     /gene="gltP"
                     /locus_tag="RHV13_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HDV1241972.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12738..13427)
                     /locus_tag="RHV13_002448"
     CDS             complement(12738..13427)
                     /locus_tag="RHV13_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDV1241973.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13521..15668)
                     /gene="fdhF"
                     /locus_tag="RHV13_002449"
     CDS             complement(13521..15668)
                     /gene="fdhF"
                     /locus_tag="RHV13_002449"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(15249..15251),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HDV1241974.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15866..17332)
                     /gene="mdtP"
                     /locus_tag="RHV13_002450"
     CDS             complement(15866..17332)
                     /gene="mdtP"
                     /locus_tag="RHV13_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HDV1241975.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDMLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATLFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17329..19380)
                     /gene="mdtO"
                     /locus_tag="RHV13_002451"
     CDS             complement(17329..19380)
                     /gene="mdtO"
                     /locus_tag="RHV13_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HDV1241976.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQVLNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAITEGQCWQSDWRISESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMAADAFTNPDYMRCAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDLLHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19380..20411)
                     /gene="mdtN"
                     /locus_tag="RHV13_002452"
     CDS             complement(19380..20411)
                     /gene="mdtN"
                     /locus_tag="RHV13_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HDV1241977.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQASDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIHAGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20430..20705)
                     /locus_tag="RHV13_002453"
     CDS             complement(20430..20705)
                     /locus_tag="RHV13_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1241978.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRIIQRANIKLAFVGIIYTALFALYAMLFWLAFF"
     gene            complement(20914..22899)
                     /gene="yjcS"
                     /locus_tag="RHV13_002454"
     CDS             complement(20914..22899)
                     /gene="yjcS"
                     /locus_tag="RHV13_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HDV1241979.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKVYYRADDYKFDINAAAPETV
                     NPSLWRQSQINGISGLFKVTDKMYQVRGQDISNITFVEGEKGIIVIDPLVTPPAAKAA
                     LDLYFQHRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPHNAQGNVGNGLGVTLATGDPSIIAPTKTIVRTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVKALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSEKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQPQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         5163 a         6052 c         6219 g         5499 t
ORIGIN      
        1 agagattaat gcgcgcggcc ttgctcaacg ccaaagccgg tctgggagcg gataaactgc
       61 gcacggaaca gttcacgctc acgcgcgcct tccgctgagt tatcggttgc cgagaagaac
      121 cagatgccga ggaatgccac ggtgatagag aacagcgccg ggtattcata cgggaagatg
      181 gctttttcgt gaccgaggat ctgtacccaa atcgtcgggc cgaggatcat cagtaccact
      241 gcggtaatta gccccagcca gccgcccatc attgcgccac gcgtagtcag tttcgaccag
      301 tacatagaaa gcagaatgat cgggaagtta cagctcgccg cgatggcaaa cgccagcccc
      361 accataaagg cgatgttctg attctcaaac agcacgccga ggataatcgc aatcacaccc
      421 aaaatcagga cggtgatttt cgatacccgc agttcttcac gttcggtcgc gccttttttg
      481 aagacgttag cgtacaggtc atgcgaaacc gccgatgcgc ccgccagcgt cagacccgca
      541 accaccgcaa gaatggtggc aaaagcaact gctgaaataa aaccgaggaa caggttgccg
      601 cccactgcat tcgccaggtg aacggccgcc atgttgttac caccaatcag atggcccgcc
      661 gcgtctttat attccggatt tgcaccgacc agcatgatcg cgccaaagcc gataataaag
      721 gtcaggatat agaagtagcc cataaacccg gtggcgtaga acacgctctt acgtgcttcg
      781 cgggcatcgc tgaccgtaaa gaagcgcatc agaatgtgcg gcaagccagc cgtaccaaac
      841 atcaatccaa gccccagcga cagcgcggag atcggatctt tcaccagccc tcccggtttc
      901 atgatgtcga cacctttcgg gtgtaccgcc atcgcttcgc tgaacagatt attgaagcta
      961 aagccgacgt gtttcatcac cataaaggcc ataaagctgg caccgaacag caacagcacg
     1021 gctttaataa tttgcaccca ggtggtcgcc agcatgccgc cgaacaggac atacatcatc
     1081 atcagcacgc cgaccagcac caccgcaatg tgatagttaa ggccaaacag cagctcgatc
     1141 agtttacctg cgcctaccat ctgagcgata aggtaaagcg ccaccaccac cagagaacca
     1201 caggccgaaa gaatacgaat cggcccttgt ttcagacgat aagaggctac atcggcaaag
     1261 gtgtagcgtc ccaggttacg cagacgttcg gcaatcagga acaaaatgat cggccagccc
     1321 accaggaagc ccagcgagta aattaagccg tcatagccgg aggtaaacac cagtgcggag
     1381 atccccaaaa atgaggcggc ggacatatag tcaccggcaa tcgccagccc gttctggaag
     1441 cctgtgatat taccgcctgc cgtgtagtag tcgctacgag aacgtacgcg ttttgatgcc
     1501 cagtaagtaa tgccgagcgt aaacacgacg aaaatcagga acataataat cgcctgccag
     1561 ttcgttggct ggcgctctac ggccccgcta atagcatccg cggcgttagc tgcgaaaggg
     1621 agtgtggcgg caagcgccgt cagaactctt ttcatgatgc ttgtacctca tgcaggactt
     1681 cgttattaag acggtcgaat tcgccgttcg cccgccagat gtagataccg gtgagaacaa
     1741 aggagatcac aatcactcca acaccaatcg gaataccgcg tgtgacgctg gtgttcggat
     1801 tcagcggggt gcccaaccag ccgggcgcga aggcgatcag taaaataaag ccgatataaa
     1861 ctgccagcat aataatcgac aggatggagg caaaccgttg ccgtttttcg actaactccc
     1921 tgaaatgcgc attgtcttct atccgctgat aaatcgtacc attcatcaca gattctccag
     1981 aggtaatgta gggattgttt taattcccgc tcccttatgg gagaaggtta acgctcgggt
     2041 aacccttgcc gaatgtaggc cggataaggc gttatacgcc gcatccggca atcaatgcct
     2101 gatgcgacgc tgtcgcgtct tatcaggcct acaaaccgtt accgactcgc atccggcaat
     2161 tgtgggttac gatggcatcg cgatagcctg cttctcttca agcagcttct cgactacgcc
     2221 aggatcggca agcgtcgagg tatcgcccag gttgctggta tcgcccgccg caattttgcg
     2281 cagaatacgg cgcataattt tgccggagcg ggttttaggc agggagtcgg tccagtgcag
     2341 cacgtctggc gtcgccagcg ggccaatctc tttacgcacc cagttgcgga cttctgcgta
     2401 cagttctggt gacggttcct ccccgtgatt aagcgtgacg taggcgtaga tcgcctgacc
     2461 tttaatattg tgcggaatac ctactacggc ggcttcggca atcttcggat gcgccaccag
     2521 cgccgactca atctccgccg tccccagacg gtgaccggag acgttcagca cgtcgtccac
     2581 gcgcccggtt atccagtaat agccatcttc atcgcgacgc gcgccgtcgc cgctgaaata
     2641 catatttttg aaggtggaga aataggtctg ctcaaaacgt tcgtgatcgc caaacagcgt
     2701 acgcgcctga cccggccagg agtcggtgat caccagactg ccttcggtgg ccccctccag
     2761 cgggttacct tcgttatcga ccagcgccgg ttgcacgccg aagaacggac gtgttgccga
     2821 accggctttc agctcggtag cgccaggcag cggggtgatc atgaaaccgc cggtttcggt
     2881 ctgccaccag gtatcgacca ccggacattt ctcgttgccg atttttttcc agtaccactc
     2941 ccacgcttcc gggttaattg gctcgcccac ggaaccgaga atgcgcagcg acgaacggtc
     3001 ggtgccttcg atcgctttat cgccttccgc catcagcgcg cgaatcgccg tgggcgcggt
     3061 atagagaata ttgacctgat gcttgtccac cacctgtgcc atacgggcag gcgtcggcca
     3121 gttcggtacg ccttcaaaca tcagcgtggt cgcgccgcag gccagcgggc cgtacagcaa
     3181 ataactgtgt ccggtcaccc agcccacatc ggcggtgcac cagtagatat cgcccggatg
     3241 ataatcaaag acatatttaa aggtcagcgc cgcgtacacc agataaccgc cggtagtgtg
     3301 cagtacgcct tttggttttc cggtagaacc ggaggtatag agaataaaca gcggatcttc
     3361 ggcgttcatc tcttccgcct ggtgctgatc gctggcttgc tcaaccaggt cgtgccacca
     3421 caggtcgcgc ccttcctgcc agtcgatgtt tccgccagta cgcttcagta ccaccacatg
     3481 ctctacgctg gtgacgttcg ggttttttag tgcgtcatca acgtttttct tcagcggaat
     3541 actacgcccg gcgcgcacgc cttcgtcgga agtgatcacc aaccgtgagt tagaatcgat
     3601 aatgcgcccg gcaaccgctt ccggcgagaa gccaccgaaa attaccgaat gcaccgcgcc
     3661 aatacgggcg caggccagca tcgcaaccgc ggcttccggc accatcggca tataaatcgc
     3721 caccacatca ccttttttaa tgcccagctc gagcagggta ttggcgaagc ggcagacgtc
     3781 gcggtgcagc tctttatagc tgatatgttt gctctggctg gcgtcgtcgc cttcccagat
     3841 gatggcggta cgatcgccgt tttcttgcag atggcggtca aggcagtttg ccgccagatt
     3901 cagcgtgccg tcctcgtacc atttaatgga cacattacct ggggcaaagg aggtgttttt
     3961 caccttctgg tacggtttga tccagtcgag aatttttccc tgttcgcccc agaaggtatc
     4021 aggtgcgttt atagattgtt gatacatcgc ctcgtactgc tgagggttta tcaggcaacg
     4081 gtctgcgatg ttggcaggaa tggtgtgttt gtgaatttga ctcatgcttt tgttctcctt
     4141 gtaggatgtt aataatatgt gtcataagcg ttaaatgtag gggtattggc agttttgttt
     4201 agtatttggg cgacagatca cgcaaaagta gaattgtgca aataaacggc agggtaattt
     4261 ttgaaggtca gcaacaaaaa ttgattaatt ctttgaggaa catgcagtta tgcatgctgt
     4321 tgaaaaagag gaagatactg actaactcta aagtggtatt ttacatgcac ttacaattga
     4381 ttaaagacaa cattcacagt gtggttattt gttacacata ggggcgagca atgtcatgac
     4441 agtgtaggtg cggttactgt cgtgaaaaac aataaaaacc gccattgcaa caatggcgca
     4501 attcggatga agcccctatg acaaggataa aaataaacgc acgccgtatc ttcagcttat
     4561 tgattccttt tttctttttc acttctgttc acgctgaaca aacggctgct cccgcaaaac
     4621 ctgtaactgt ggaagcgaag aatgaaacct ttgccccgca gcatcccgat caatatctct
     4681 cctggaaagc cacctcggaa cagtcagagc gtgttgacgc cctggcggaa gatccacggc
     4741 tggtgatcct gtgggcgggg tatcccttct cgcgcgacta caacaagccg cgtggacatg
     4801 cctttgctgt gaccgatgtg cgtgaaaccc tgcgtaccgg tgcgccgaaa aacgctgaag
     4861 atggtccgct accgatggcg tgctggagtt gtaaaagccc ggatgtggcg cgtctgatcc
     4921 agaaagacgg cgaagatggc tacttccacg gtaaatgggc gcgcggcggc ccggaaatcg
     4981 tcaacaactt aggttgtgcc gactgccata acaccgcctc accagagttc gccaaaggca
     5041 aaccggagtt aaccctttcc cgtccgtatg cggctcgcgc gatggaagcc attggtaaac
     5101 cttttgagaa agccggacgt ttcgaccagc aatcgatggt ttgcggtcag tgccatgtgg
     5161 agtattactt cgacggcaaa aacaaagcgg ttaaattccc gtgggatgac ggcatgaaag
     5221 tcgaaaatat ggagcagtat tacgacaaaa ttgccttctc tgactggact aactccctgt
     5281 cgaaaacgcc aatgctgaaa gcgcagcacc cggaatatga aacctggaca gcgggcattc
     5341 acggtaaaaa caacgtgacc tgtatcgact gccatatgcc aaaagtgcag aacgccgaag
     5401 gcaaactcta caccgaccat aaaattggta atccgtttga taacttcgcc cagacttgtg
     5461 cgaactgcca tacccaggac aaagctgctt tgcaaaaagt ggtcgcggaa cgtaagcagt
     5521 cgattaacga cctgaaaatc aaggttgaag atcaactggt tcacgctcac ttcgaagcga
     5581 aagcggcgct ggatgcaggc gcgacggaag ccgaaatgaa gccgattcag gacgatatcc
     5641 gtcatgccca gtggcgttgg gatctggcga tcgcttccca cggcattcat atgcacgcac
     5701 cggaagaagg tctgaggatg ctcggtacgg cgatggataa agcggcggat gcgcgcacca
     5761 aactggcgcg cctgctggcg accaaaggca tcacccatga aatccagatc ccggatatct
     5821 cgaccaaaga gaaagcccag caggccattg gcctgaacat ggaacaaatc aaggccgaga
     5881 aacaggactt catcaaaacg gtgatcccgc agtgggaaga acaggcacgt aaaaatggtc
     5941 tgttaagcca ataaccccgt tccgcctcgc aaggggcgga aaacacaatg gagtgaatat
     6001 gagcgtatta cgttcgttat taactgctgg ggtgctggcg tcgggcctgt tgtggagcct
     6061 gaacggaatt accgctaccc ctgcggcgca ggcatctggt gatcgctacg aagttaccca
     6121 gcagcgtaac ccggatgccg cctgtctgga ctgtcataag ccagataccg aaggtatgca
     6181 tggcaaacat gcttccgtta tcaacccgaa taacaaactg ccggtcacct gcaccaactg
     6241 tcacggccag ccatcaccgc aacaccgcga aggggtgaaa gatgtgatgc gctttaacga
     6301 gccgatgtac aaggttggag agcagaacag cgtctgtatg tcctgtcatc tgccggaaca
     6361 gttgcaaaaa gcgttctggc cgcacgatgt ccacgtaacc aaagtggcgt gcgccagctg
     6421 ccattccctg catccacagc aagatacgat gcagacgtta agtgacaaag gacggattaa
     6481 gatttgcgtc gattgccaca gcgatcagcg caccaatccg aactttaacc cggcgtctgt
     6541 tccgttgctt aaggagcagc catgacctgg tctcgtcgcc agtttctcac cggcgtcggc
     6601 gtgctggcag ccgtcagcgg caccgcagga cgcgtggtgg cgaaaacgtt aaatatcaat
     6661 ggggtgcgtt acggcatggt gcatgacgag tcgttatgca tcggctgtac cgcctgtatg
     6721 gatgcttgtc gggaagtgaa caaggtgccg gaaggcgtct cgcgcctgac gattattcgt
     6781 agcgagccgc agggcgaatt tcctgatgtg aaatatcgct tcttccgtaa gtcttgccag
     6841 cactgcgatc atgcgccgtg cgttgacgtc tgcccgaccg gtgcgtcttt tcgtgatgcc
     6901 gctagcggga ttgtcgatgt taatcctgat ctctgcgtcg gttgtcagta ctgcatcgcc
     6961 gcctgtccgt accgcgtgcg ctttatccat ccggtcacga agacggcgga caaatgcgat
     7021 ttctgccgta agaccaattt gcaggccggt aagctgcccg cgtgcgttga ggcttgcccg
     7081 acgaaggcgc tgacgtttgg caatctggac gatcctaaca gtgagatttc gcaactgctg
     7141 cgccagaagc ctacttaccg ctacaagctg gcgctgggaa ccaaaccgaa gctgtaccgc
     7201 gtaccgttta aatacgggga ggtgagccaa tgacgcagac ttccgcattt cattttgaat
     7261 cgctggtgtg ggactggccg attgccatct acctgttttt gattggtatt tctgctggtc
     7321 tggtgacgct ggctgtgctg ttacgtcgct tctacccgca ggcgggcggt gcagacagca
     7381 cgttgctgcg caccacgctg attgtcggac ctggcgcggt gatcctcggt ctgttgatcc
     7441 tcgtcttcca cctgacaaga ccgtggacct tctggaagct gatgttccac tacagtttta
     7501 cctcggtgat gtcgatgggg gtgatgctgt ttcagcttta catggtggtg ctggtgctgt
     7561 ggctggcgaa aatctttgaa catgatttgc ttgccctgca acaacgctgg ttgccgaagc
     7621 tggggatcgt gcaaaaggtt ctgagcctgc taacgcccgt tcatcgcgga ctggaaacgc
     7681 tgatgctggt gttggcggtg ctgctggggg cttataccgg ctttctgctg tcggcgctga
     7741 aatcgtatcc gttcctcaat aacccgatcc tgccggtgct gttcctcttc tccggcatct
     7801 cgtccggtgc ggcggtggcg ctgatcgcca tggcgatacg ccaacgcagt aacccgcatt
     7861 ccacggaagc gcagtttgta caccgtatgg aaatccccgt ggtatggggt gaaatcttcc
     7921 tgctggtggc gttttttgtc ggtctggcct tgggcgatga cggtaaagtg cgtgcgctgg
     7981 tggcggcatt gggtggcggt ttctggacgt ggtggttctg gcttggtgtc gccgggctgg
     8041 ggttgattgt accaatgttg ctcaaaccgt gggtcaatcg cagttccggc attcctgccg
     8101 tgctggcggc gtgtggggcc agcctggtcg gcgtgttgat gttgcgattt ttcattctct
     8161 acgccgggca gttaacggtg gcgtaagcca gaaaagaggt ggtttctgga cgtattcctt
     8221 cctgaagtcg gttttctggc attgttgtta agtctcgggg tcaacgtgtt gaccccgttg
     8281 acggccttcg cgggagtgcg gttgcgctgg cctgccatga tgcgactcac ttgcatcggc
     8341 attctggcac agttcgcgct cctgctgctc gcctttggcg tactgacgta ttgttttctc
     8401 atcagcgatt tctcggtcat ttatgtcgcc caacatagct acagcctact gtcgtgggaa
     8461 ctcaaactgg cggcggtatg gggtggtcat gaaggttcgc tgctgctttg ggttctgctg
     8521 ctttccgcct ggagcacgct gtttgcctgg cattatcggc agcaaaccga tccgctattt
     8581 ccgctgacgc tagccgtttt atctctcatg ctcgccgcac tgctactgtt tgtggtgctg
     8641 tggtccgatc ccttcgtgcg gatatttcca ccagcaatcg aaggccgaga tctcaatccg
     8701 atgctgcaac atcccggtct tatctttcat ccaccgctgc tttaccttgg ctatggcggt
     8761 ttgatggtag cggcgagcgt ggcgctggcg agtctactgc gcggcgagtt tgatggtgcc
     8821 tgcgcccgaa tttgctggcg ctgggcgtta cctggctgga gtgcattaac agcggggatc
     8881 atcctcggtt cctggtgggc ctattgcgaa ctgggctggg gcggctggtg gttctgggat
     8941 ccggtggaaa acgcctcttt attaccctgg ctttctgcca ctgcgctgct gcacagttta
     9001 tccctgacac gccagcgggg gattttccgc cactggtcgc tgttgctggc gatagttact
     9061 ctgatgctgt cgctgctggg caccttaatt gtccgttctg gcattctggt ttcggttcat
     9121 gcgttcgctc tggataacgt tcgcgccgtg ccgttgttca gcctgtttgc actgattagc
     9181 cttgcgtctc tggctctgta tggctggcga gcgcgggacg gtggcccggc ggtgcgtttt
     9241 tcggggttat cgcgggaaat gttaatcctc gctacgctgt tgctgttttg cgcagtgcta
     9301 ctgatcgtgc tggtgggaac gctttatccg atgatttacg gcctgctggg ctggggacgc
     9361 ctctccgttg gcgcaccgta ttttaaccgc gcgacgttac cgtttggtct gttgatgttg
     9421 gtggtgattg tgctggcgac gtttgtctct ggtaaacgcg cgcagcttcc agcgctgctg
     9481 gcgcatgcgg gcgtgctgtt atttgccgca gggatcgttg tctccagcgt cagccgtcag
     9541 gaaatcagcc tgaatttaca gccgggtcag caggtgatgc tggcaggata caccttccgt
     9601 tttgagcgcc tcgatctaca agccaaaggc aattacacca gcgaaaaagc gatagtggcg
     9661 ctgtttgacc atcagcaacg cattggtgaa ttaacgccgg agcggcgttt ttacgaagca
     9721 cgtcgtcagc aaatgatgga accgtcaatt cgctggaacg gcatccatga ctggtatgcg
     9781 gtcatgggtg aaaaaaccgg agcggatcgt tacgcttttc gcttgtatgt acaaagcggt
     9841 gtgcgctgga tctggggggg aggattgttg atgattgcgg gcgcattgtt aagcggatgg
     9901 cgggggagga agcgcgatga ataaaggact tctcacgcta ctgctgttat ttacctgttt
     9961 tgcgcgcgct caggttgtag acacctggca atttgccaat ccgcaacaac agcaacaggc
    10021 gttaaatatt gccagccagt tacgttgtcc gcagtgccaa aatcagaact tactggaatc
    10081 caatgcgccg gtggcggtca gtatgcgcca tcaggtttac agcatggtgg cggaggggaa
    10141 gaacgaagtc gaaatcattg gctggatgac cgaacgctac ggagattttg ttcgctataa
    10201 cccaccgtta acgggtcaga cgctggtgtt gtgggcgctg ccagtggtgt tgttactgct
    10261 gatggcactg atcctctggc gagtgagggc gaagcgatga aacagcctca aataccggtg
    10321 aaaatgctga caacactcac gatattgatg gtattcctct gtgtcggcag ttatctgtta
    10381 agcccaaaat ggcaggcggt acgtgcggag tatcagcgtc agcgcgatcc gctacatcag
    10441 tttgccagcc agcaaacccc ggaagcgcag cttcaggcat tgcaggataa aatccgcgct
    10501 aatccgcaaa acagcgaaca gtgggcgtta ctgggcgagt actatctgtg gcaaaacgat
    10561 tacagcaatt cgctgctggc gtaccgtcag gcgttgcaac tacgtggtga gaacgctgaa
    10621 ctgtatgcgg cgctggcgac ggtgctttat taccaggcca gccagcatat gaccgcccag
    10681 actcgcgcaa tgatcgacaa agccctcacg ctggactcta acgaaatcac cgctctgatg
    10741 ctgctggctt ccgatgcgtt tatgcaggcg aactacgcgc aagccatcga actatggcaa
    10801 aaagtgatgg atctcaactc accccggatc aaccggacac agctggtcga gtcgattaat
    10861 atggcgaaat tgttgcagcg gagatcggat taatcatctg gtgatgaaga ttatgttatt
    10921 gagctgcata aaaaataatc gaatgaacat gtgccaaaaa taatcactaa tcagtattat
    10981 tgcagattaa acaaataaaa atctttccat aacaaatggt tattcattaa tcctgccata
    11041 aactcgcgtt attatgcatt aatgcagcga aaagctctgt tgttaaaggg ttgcgcaaca
    11101 taccgcgcaa atgatactga tcataagcgt taaaaaaatc tacaaaccaa cgcaacacaa
    11161 ttcatgccct ggcagtatgt cacgttctcg cgtttctgaa cggggaacgg cgctccattg
    11221 aggaagttat tctgatgaaa aatataaaat tcagcctggc ctggcagatt ctgtttgcta
    11281 tggtgctggg cattctcctg ggaagctacc tgcactacca tagcgacagc cgcgactggc
    11341 tggtcgtcaa tttgctctct ccggcgggtg atatcttcat ccatctgatc aaaatgattg
    11401 ttgtgccgat tgtgatctcc acgctggtgg tgggtatcgc gggtgttggt gatgccaaac
    11461 agctcgggcg tattggcgcg aaaaccatta tctacttcga ggtgatcacc accgtcgcca
    11521 tcattttggg gatcactctg gcgaacgtct tccagcccgg tgccggggtg gatatgtcgc
    11581 agttggcgac cgtcgatatc tcgaaatatc agagcactac ggaagcggta caaagcagtt
    11641 cccacggcat tatgggcacg attttgtcgc tggtgccgac gaacattgtg gcgtcgatgg
    11701 cgaaaggcga aatgctgccg atcatcttct tctcggtgct gtttggtctg gggctttctt
    11761 ccctgcccgc gacgcatcgt gaaccgctgg tgaccgtgtt ccgctccatc tctgaaacca
    11821 tgtttaaagt gactcacatg gtgatgcgtt atgcgccggt gggtgtgttt gcgctgattg
    11881 cggtgacggt ggctaacttt ggtttctctt ctctgtggcc actggcgaaa ctggtgctgc
    11941 tggtgcattt cgccattctg ttcttcgcgc tggtagtgct gggaattgtg gcgcgcctgt
    12001 gcgggttaag cgtctggatc ctgattcgta ttctgaaaga tgagctgatt ctggcgtact
    12061 ccactgccag ctctgaaagc gtactgccgc gaattattga gaagatggaa gcctacggag
    12121 caccggcgtc gatcaccagt ttcgtggtgc cgaccggtta ctcttttaac cttgatggtt
    12181 cgacgctgta ccaaagtatt gccgctattt tcatcgctca gctgtatggc attgacctgt
    12241 ccatctggca ggaaatcatt ctggtgctga cgctgatggt gacctcgaaa gggattgctg
    12301 gcgtgccggg cgtgtcgttt gtggtgttgc tggcaacgct gggtagcgta ggtattccgc
    12361 tggaaggtct ggcgtttatt gctggtgttg accgtatcct cgacatggcg cgtactgcgc
    12421 tgaacgtggt gggtaatgcg ctggcggtgc tggtgattgc caagtgggaa cacaaatttg
    12481 accgcaaaaa ggcgctggct tatgagcgtg aagtgctggg caaatttgat aaaactgcgg
    12541 atcaataatt gaagattacc ggggttatcc cccggcaatg tgtggatgcc tgatgcgacg
    12601 ctggtgcgtc ttatcaggcc tacgtcagac agcgcaatag cctgatttag cgtgattttg
    12661 taggtcggat aaggcgttta cgccgcatcc gacatcaacg cctgatgcga cgcttaacgc
    12721 gtcttatcag gcctacctta accgttagtt aatttttcaa actcttcaca cccggtatca
    12781 aacccttcca tacagctcag gttcaaccag tgcagcgcct tttgtttgtt cttctcgata
    12841 aaaccttctt caccgtttaa gaacatcatt cccgcccagt actcggaata accggttcgg
    12901 gaaattgcag agctgcgttt gaaataccag gttgccttgt catcgtcggc cttaatgccc
    12961 acgccgttgg cgtaaatcaa accaagcagc atttgggcat cgacggcaga gtcgttctcc
    13021 agatcttccg aggcgttttc taacagcgaa atcgcctttg gataatccgg tttacccgct
    13081 tgagtattta ccagaatatg cgccagcgtt acttcacctt ctttactgcc cgcttgcgcc
    13141 gctttctctg caagaacttt tgcttgtgga tagtccagac tgaccggatt ggtgattttg
    13201 atctgcgcca gcagcgcgca ggcatcagca tcaccgctgt cggcggcttt ctgtgcccaa
    13261 tattcggctt tgctcaaatc gccggagcta aaccagctgt cggcaagaaa atattgtgcg
    13321 cgtcggtccc cggcctctgc tgcctttaaa tactggctgc caggctcgga gtcgttcgcg
    13381 tgggcaaaga atgtcaaaaa caacatcaat gcgataattt ttttcatttt gataatacga
    13441 ttgggtaggg agtaaccagt ataaagagtt cgaatggata aaaaaacagc ctccgaaagg
    13501 aggctgtaga aaggactgta ttacgccagt gccgcttcgc gcaggcgagt tttcaacttg
    13561 ttgtactcgt caatcacgta ctgctcggcg gcacgctgat cggcgatcgg ctcgacgcgc
    13621 acggcgcagt atttgtattc cggcgttttc gtaatcgggc ttaagttttc ggtaaccagc
    13681 tcgttacagg caccaatcca ccactggtag gtcatgtaaa tcgccccttt gttcggacga
    13741 tcgctgacct gcgcacgggt gataattttg cctttacgcg agtgcaccca aaccaatgcc
    13801 tcatcttcaa tacccagacg tttggcgtct tcggtattga tttgtgcgta gccaggttca
    13861 tcagccagcg ccgccagtgc cgcacagtta ccggtcatcg aacggcaaga gtagtgacca
    13921 acttcacgca ccgttgacag taccatcggg tactcgtcgg tgagtttgtc gattggcgct
    13981 acccagtcgc aggtgaagaa ctgcgccaga ccgttcgggg tatcaaactt ctctttaaac
    14041 agataagaag tcccctgatc ggcatctgaa gtatcgcggc aaggccactg aatgaagccc
    14101 agttcgccca ttttctcgta agtcgcaccg tagaaatccg ggcacagatg acgcaactca
    14161 tcccagatct cctgggtgtt gttgtagtgc atcggataac ccatacgggt ggcgatttca
    14221 ctgatgattt gccagtccgt tttcagatcc catttcggtt caactgcttt gaagaaacgc
    14281 tggaagccac ggtcagccgc agtaaacacg ccttcatgct cgccccacga cgttgacggt
    14341 aaaataacat ccgccgccga cgcggttttg gtcataaaga tgtcctgaac gatgaccagt
    14401 tccagatctt caaaggcttt acgtactgcc gacagctccg cgtcagtttg tagcggatct
    14461 tcgcccataa tgtacgcggc acgcacttcg ccatgcgctg cgcggtgcgg cagctcgctg
    14521 atgcgataac cggtatgagc aggcaggctt tccacgcccc aggctttggc gaatttctcg
    14581 cggttagccg gatctttcac gtactggtat cccggatacg tatccggcag cgcgcccata
    14641 tcgcaggcac cctgcacgtt gttctgacca cgaaccgggt ttacacccgc atgcggctta
    14701 ccgaggttac cggtcagcat cgcgaggctg gtcagagaac gcacggtttc cacgccctgg
    14761 tagaactggg ttacacccat gccccacagg atggcggcgc ttttcgcctg ggcatacatc
    14821 cgtgccgcct gacgaatctc actggcgctg acgccggtga tatcttcaac cgactccggc
    14881 gtgtagcctt caacgatttt acgatactct tcaaagcctt ctgtacgtga agcgacgaac
    14941 gctttgtcgt acagattttc ttcaataatg acatggccca tcgcattcaa cagcgcgatg
    15001 ttcgagccgt ttttcagtgc aatgtgcatg tcagcaatgc gcgcggtttc aattttgcgc
    15061 ggatcgcaga cgataatttt cgccccgtta cgtttagcgt taattacgtg attcgctacg
    15121 attgggtggg aatccgccgg gttgtacccg aaaacgaaca ctaaatcggt attatcaatt
    15181 tcgttaatag cattgctcat tgcgccatta ccgaccgatt ggtgcagacc tgcaaccgat
    15241 gggccgtgtc agacacgagc gcagcagtca acgttattgg taccaataac ggcgcgcgca
    15301 aatttttgca ttacatagtt ggtttcgtta cccgtaccac gcgaggagcc ggtcgtctgg
    15361 atggcatccg gaccgtactt ctctttgatg gcgctcaggc gctcggcaac gtaattcagt
    15421 gcctcatccc aggaaacagg ttcgagtttg ccgccacgct ggcgacggat catgggggtt
    15481 ttcaggcgcg gggtcaggat ctgggtatcg ttaatgaaat cccagccata ataacccttc
    15541 agacacaggg taccctggtt ggttttcccc tgcgctgcct ccgcccggac gattttgccg
    15601 ttatcgacga ccaggttgat tttgcaacct gatgcgcaat aggggcaaac cgtgacgact
    15661 tttttcatcg gtctcgctcc agttaatcaa atcacgcata cgcgctctcg actacagtat
    15721 gcatctttta tgccacattt tatttggggt cattccctga tattacgggc actatttatt
    15781 caaaactctg acgaaaaaca ggctgtcgtc agttttgacg tgacgaaacg aaataccgcg
    15841 tgacagccat cacgcggcag acattttatt ttttctcgac gacgggagcc gcctgatacc
    15901 cgccgcccag cgatttcatc aactgaatgc tttggatcac ccggcggctg tccagcatca
    15961 gtaatgacat ctcttcggca agtactggca atcgggcttc agtggcctgt aagcggctgg
    16021 ttaagccgcg ctgataggcg gcctcggcag cgcgctgggt gaagagcgtt gcttccacgc
    16081 gttcagcctg catttctcgc tcgtcgttga gcgtttgcag acgcgtgccg ttgacggcaa
    16141 cgtcacgcac cgcgttcagt actgactggt tgtaacgttc aatcatcatg ttgctggcgg
    16201 cgcgcgtacc ttcgagattg gcattcaacc gtccaccgtc aaacagcggc aatttcagac
    16261 ccgggatgaa gttgaactgg cgactggttt ttttgaataa ggtatccagg tggatggagt
    16321 ccagaccgaa aaacgctttg atatcaaagc ttgggtagaa cagcgcccgc gcggaatcca
    16381 cctgatctaa tgacgcctga acataccagc gcatggcttg cagatccggg cgtctggcga
    16441 gcaactcata agagagtgtc gccggaatgc cggtctggac tcgtggtaat gccaccggtt
    16501 tgatctccgg catatcgctg gctcccgcgc caatcaatgc gcgcagagat tctcgcgttt
    16561 cggtgatttg ccctttgacg gcagcaattt gtttatcgac cgccagaatc tgtgcccgcg
    16621 cgccgtggaa aggtacttgc gcttccagac cgtgcgccac tttactttgg tgcgctttca
    16681 ccgcgtaatc aatcacatcg cgagtttgtt ctaacatatc gagcatctga tagctggcct
    16741 gcatactgta ataaagctgc gctacgcccg tggtcagcga tagctctact gctgcggttt
    16801 ctgccagcgc ggcattatgc gcgccaatgg cggcggcaac cgctgagcga tgcacacccc
    16861 acaaatcaag atccagtccg gcaaacaaac ctactgtggc ttccgtatag tacggcccgt
    16921 ccatacccag tgctggcgca tccatcgcat aagggcttaa aaagccgttc gccgagacgc
    16981 gttggcggtt gagcatccct aacgctgcga cctgtaattg tgaaccggca tctaacaaat
    17041 cggcctggga ctgcgctttt tcttcccgca gtttcgcttc ggcgagggtg tgtgaaccac
    17101 ttagcgtccg ttggatcagc gcatccagct gcgggtcatt gagttgtttc caccactgcg
    17161 cctgcggcca gccggagctg gcaagatgaa tatcatcggc cagtttgatt tgttccggtt
    17221 tgagctgttg atgaggcgca gaatccttgc gtaccagggc acagccggaa atcagcgtcg
    17281 tgctgccgag aatgctgcac aacagcagac gtgaaagttg acgattgatc attgcgtggc
    17341 tccttgtgcc tgttccgtgg cgggcgttaa tgccggggct gtccagtctg gcaagcgggc
    17401 gaaaagctgg cagacgtgtt gctcctgttt taataaggtg ggcaggatgg cctgcgaggc
    17461 gggtgtctct tcaagcgtta tttgaggaga acggctgagt gcggttgcca gaccggcagc
    17521 gtatttttcc agagcgtcgg caaactgacc tgctctgtcc ggctgtagtg cgttatccag
    17581 cgcctgcgcc gagttccagg tggcatccca ggcgtgaaga agatcgcggc cctgacgaat
    17641 aaccgtttgc gaacgttcaa tcagtaaggc gcgttcttcg ctgtccagtt gacgctccag
    17701 cgccacgcgt tggcacatct cttcacaggc attaaacgcc gcatgcagac ctatacgaat
    17761 ttgcagataa gtgcgcagag ccgtgacttc ctgctggcgt gggatccgca ttactttact
    17821 tagcataccc agcgcaccgg ccagtttttg cggcagcgtg cgcgcttcac tttcaggcca
    17881 gacaaaggtg taaatcaccg cagacaccac ggtaccaatg atgataccca gggcgcgatc
    17941 gcgaatttcc accaggtcgt acaccggacc aaaaacgttt tcgagcgtgg cgagcgcgaa
    18001 ggtgaccacc atctgtgtgc cgatataaga agagcgttca gagctggtgg caatccatgc
    18061 gcccaacagg aaaatcggtg ccagcacaaa cagcaattcg acaatattgt ccagccaggg
    18121 catgaccagt agcgtgaata acagcgccag aatcgcgccg caaaaggccc cgccaaaacg
    18181 cagcaccatc ttctggtacg atgaaccgac attcggatta gcgacgatca cgcaggtcag
    18241 catacaggtg tgaatgcctt cccagtccac gccgctgtag aaggtgtaac agatcaaaca
    18301 ggcgagcagc gtttttaccg cgcagcgcat atagtctgga ttggtaaaag catcggcggc
    18361 cattgatggc ggtttggctg cgggcgttgg cggcgtattc gggtccatct gaccaagctg
    18421 taacagtgtc tggcagatat tctccaggtt acattcccgt gccgccatcg cttcactttc
    18481 actgatccgc cagtcgcttt gccagcactg accttcggta atggcatgct gcagcttgtt
    18541 gatttctgaa gctaattttt gtcggaattc aataattgcc tgagaatcag caaaagaggt
    18601 gggatcgtag cgattcagcg tcgagtaaat gtaggttacc gttgccacgc agctttgcca
    18661 ccatgcactc tgggttcgcc agttggcatc gtccgcgagg caaaagacat tgagtttttg
    18721 cagcgccagc gcctctcttt caatccgcgt ttcgggtagc ggtgcgaggc tgtccgtcag
    18781 gtggctaatg gcatcatcaa gccgatcatt aagcacctga tgcatttgcg aaatggcacg
    18841 actgggaaac cacagcacgc cgattaacgt catcagcaag gttggataga ggccaacaac
    18901 gatacaccac agcgttaagc gcacgaccac ttccggatag tcgagcatgg cggggaaggt
    18961 ttgcccgtaa atagcgacaa tggcgacggc gaaaaagacc agccccaagc gatgggtgcg
    19021 catcaaaaac atgcagccca tcaggatcgg tccggcgatg atcaagcgga tcaacggttc
    19081 gccgtatgac catttgtaga tcaaaaacag gctgccgatc tccagcaccg tggcaaccac
    19141 aaacaagatc gcgacaaatt tggtgtaaaa cgcgttcgac tgaataccgt aaaacagcac
    19201 tgccagcgat aacgccacaa aagggatctc aaaagtcatc gagatcagaa tcaccagcag
    19261 gcagcctacc cagagttgca cggtctgcgg cacgcgacct ggtcgccgct cgcttaactc
    19321 ttcatgaaag aacgccagca gcctgaccac cggtaatggc agggagttga gcgcgctcat
    19381 tattgtggct caaggttagc gactgccgaa gcgccgatgc ggaacatttc cgggtcaggt
    19441 ttatcaacca tgattttgac cggaaaacgc tgggcaacgc ggacccagtt aatagaacgt
    19501 gatactttcg gcagaccgcc cagcaccagg ccgccgtcat ccggcagtac gccgtagcca
    19561 atagaatcca ctttaccttc aaaggttttg ccgctgtcgc tcatcaggcg aatggttgca
    19621 ggcgtaccag catgaatgtt tttcaaatcg gtttcgcgga agttagcgat aacgtaccag
    19681 tgacgagtgt cgattagggt aaaaataggg cgcatggcag aagcaaattg cccgacggag
    19741 gttttgaggg aaatgacccg gccatcaaac ggcgcgcgaa cggtcgccat ttccagatgc
    19801 agtttggtca gggcaatatc cgcttcgacc gccgcacgtt gcgccaccag tgcatcgacg
    19861 ccgctgacgg cgctggcggc tgactgtgcc tgtaacaata cggcattaag atcggcttct
    19921 gccgcgcgct gcgccgttct tgcgcggtca acatcttctg cagaaacaaa accttctttc
    19981 agcagcggct cggtgcgacg taatgtatcg ctggcctgtt tcgcggcagc acgggctttt
    20041 tctaccgtgg cattaaccga gtcggcacca aactgttgcg cgtcaacgct acgctgggtg
    20101 agcataattt gtttatccag cgccgcgagg gaggcttcag ctttcgccag attggcttcg
    20161 tacgggcgcg ggtcaatgcg gaacagcaaa tcgccctgtt tgactgcctg gttgtcggtg
    20221 accgccagtt ctacaatgcg gccgctgact tccggcacca catcaatggt atctgctgac
    20281 gcgtaagcgt cattagttga tggcgcactg tctacgcgcc agataacgaa aacaagggca
    20341 accagcgcca acgcaaccac taacagagca gggaatttac tgcgaggagc ttttttcggc
    20401 gtactttcca tgagcgcgtc tcaaatagat tagaaaaatg ccagccagaa cagcatggcg
    20461 tagagagcaa aaagggcggt ataaataatt ccgacaaatg ccagcttgat atttgcccgc
    20521 tgaataattc gcctcgtgat gagcgtcaaa ataaggctgg caatggcgca gaaaaaccag
    20581 ctgggataat aagcgccgat caccgggatt gccggagata atgagcatcc cgaaaccatg
    20641 aagacgggaa ttatccaggc tgtttttttg agttgcatcg ccatgcgaga gagaactgtt
    20701 ggcatattaa atacatcctg tctgctgcga acaatatctg tagtgaatat caatatccat
    20761 tgaatgcgtc agtatggcta ttttcttgat gaataaaaat agctttattc agccaccgca
    20821 ttcataactt atatatttta tttattaatt ggctgtatat atttttaaaa taaatgtttt
    20881 tgggggcttc agcgccccca aaagcgagtg acattaattt ggggttacga tattcaccca
    20941 caaatcgaaa ttatccagac aggcgataat ttcttccagt ttcgcgccat tgccaataat
    21001 tttggctttc ttcgctttca ccagatccgc catttttgcc tgtccggtca gcacggcgtg
    21061 cagatcttca cggctgatgt agaaagaggc gtcggcttgt ggttgcagtg ttttacggta
    21121 gttaagcacg ctatcgttca gcgtcaggtt gaggttatcg ccgttgctca tattgaagtt
    21181 caggctgata tttttacccg cggctttttc gctatcgagg cgaacggaca taaagtcgaa
    21241 cagcatttcg accgacattc cgcgaatggt gtccggggaa ccggtggtgc cgtggctgaa
    21301 cttatgcacc ccttcgcgca gctctttcgc gccggtcagg tagaaaccgc gccatgtggc
    21361 ggactcggcc tgatagccca gctgttcaaa gttattcgct tgcagattct tcgcgacctg
    21421 gtcacccggg ttggcggcaa tcacctgttt cagcagttct gccgaccagc ggtaatcacc
    21481 ttgcttgttc gcttcttgcg ccaggttgat gacacgggca gaaccgccca gcgctttcac
    21541 gtaacgtttg cccatctcta cctgaccata cggatgcagg ttagccgggt taccgtcgta
    21601 atagccaaga tagaagttat acaccgcgcg ggcgttgtgg ctgacagaac cgtaataacc
    21661 gcggctggcc cagttattgg caagcgcagg cggcagctta atcatgtcgc cgatttcatt
    21721 catggtgtag ccctggttcg ccagatgcag ggtttgatcg tgaatatact tgatggtatc
    21781 gcgatattta ccaatataat cattgatatg cttattgccc cagaccggcc aggtgtgcgg
    21841 cataaacagt acttccgcgt cgttacccca catatccagc gtttcgttca ggtactctgt
    21901 ccacttgctg gtatcgcggg ttttcgcgcc gcgcagagtg tagaagttgt gcagggtatg
    21961 cgtggcgttc tcggcggtac acagggcttt cagggccgga atatagaagt gcatttcggc
    22021 tggggcttcg ctgcctgggg tcatcaggaa gtcaaactcc aggccgtcga taatcatctt
    22081 ctcgccagtt ctgacgatag ttttggttgg cgcaataatg ctcgggtcgc cggttgccag
    22141 cgtcacgcca aggccattac ccacgttgcc ttgcgcgttg tgcggcagta acagaccgta
    22201 agagtagagc gcacggcggc tcatgatgtt acccgccagc acgttttcgc tgatggcttc
    22261 gtccataaag ccagcagggg caatcacctg aactttgccg gatttaacat cggcttcaga
    22321 gataatacct ttcacgccac catagtggtc agtgtggctg tgagtgtaaa taacggcaac
    22381 aatcggtttt tgcggacgat gctggaagta aagatcaagt gcggcttttg cggcaggcgg
    22441 cgtcaccagc gggtcgataa caataatgcc tttctcgccc tcaacgaacg taatgttaga
    22501 gatatcctgg ccgcgcacct gatacatttt atcggtgact ttgaacaggc cagaaatgcc
    22561 gttgatctgc gactgacgcc acaggctggg gtttacggtt tccggcgcag cggcattaat
    22621 atcaaatttg taatcgtctg cgcggtagta aactttaccg ttcgcatcac gcagaatacc
    22681 ttcatccagc agcggggcga taaatccacg ctgggcatcg tcaaaatcct gacgatcgct
    22741 aaacggtaat gatttggcgt aattctgatt aacctgttgg gtatattgag tggcggcttt
    22801 cgcttcctct ttcgcgttag cggtatttac catcatgccg ctggcggcag ttaacgcaat
    22861 aacaatcctg ctcaaacgga ataaccgaga gttattcata taaatctcca tttatcattt
    22921 atgtcgtaaa tat
//
LOCUS       DAPGOF010000074        22546 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.074, whole genome shotgun sequence.
ACCESSION   DAPGOF010000074 DAPGOF010000000
VERSION     DAPGOF010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.074"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            36..578
                     /locus_tag="RHV13_002455"
     CDS             36..578
                     /locus_tag="RHV13_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV1241980.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGSVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTNFHINYY"
     gene            661..1362
                     /locus_tag="RHV13_002456"
     CDS             661..1362
                     /locus_tag="RHV13_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDV1241981.1"
                     /translation="MKTCITKGVVTVSLTAILLSCSSTWAAGKGGVGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            1387..3987
                     /locus_tag="RHV13_002457"
     CDS             1387..3987
                     /locus_tag="RHV13_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HDV1241982.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQACVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQATLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSHGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            3978..5048
                     /locus_tag="RHV13_002458"
     CDS             3978..5048
                     /locus_tag="RHV13_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV1241983.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            5060..5602
                     /locus_tag="RHV13_002459"
     CDS             5060..5602
                     /locus_tag="RHV13_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV1241984.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            5610..6125
                     /locus_tag="RHV13_002460"
     CDS             5610..6125
                     /locus_tag="RHV13_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV1241985.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVLLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            6091..6828
                     /locus_tag="RHV13_002461"
     CDS             6091..6828
                     /locus_tag="RHV13_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HDV1241986.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEVSTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            6939..7949
                     /gene="pyrD"
                     /locus_tag="RHV13_002462"
     CDS             6939..7949
                     /gene="pyrD"
                     /locus_tag="RHV13_002462"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HDV1241987.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            8123..8665
                     /gene="zapC"
                     /locus_tag="RHV13_002463"
     CDS             8123..8665
                     /gene="zapC"
                     /locus_tag="RHV13_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HDV1241988.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVAHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8662..9771)
                     /gene="ycbX"
                     /locus_tag="RHV13_002464"
     CDS             complement(8662..9771)
                     /gene="ycbX"
                     /locus_tag="RHV13_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HDV1241989.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTAHITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            10015..12123
                     /gene="rlmKL"
                     /locus_tag="RHV13_002465"
     CDS             10015..12123
                     /gene="rlmKL"
                     /locus_tag="RHV13_002465"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HDV1241990.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLLVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            12135..14042
                     /locus_tag="RHV13_002466"
     CDS             12135..14042
                     /locus_tag="RHV13_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV1241991.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRQVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKNEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            14172..15425
                     /gene="pqiA"
                     /locus_tag="RHV13_002467"
     CDS             14172..15425
                     /gene="pqiA"
                     /locus_tag="RHV13_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HDV1241992.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCGTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            15430..17070
                     /gene="pqiB"
                     /locus_tag="RHV13_002468"
     CDS             15430..17070
                     /gene="pqiB"
                     /locus_tag="RHV13_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HDV1241993.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFASNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            17067..17630
                     /gene="pqiC"
                     /locus_tag="RHV13_002469"
     CDS             17067..17630
                     /gene="pqiC"
                     /locus_tag="RHV13_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HDV1241994.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVAVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            17886..18053
                     /gene="rmf"
                     /locus_tag="RHV13_002470"
     CDS             17886..18053
                     /gene="rmf"
                     /locus_tag="RHV13_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HDV1241995.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(18123..18641)
                     /gene="fabA"
                     /locus_tag="RHV13_002471"
     CDS             complement(18123..18641)
                     /gene="fabA"
                     /locus_tag="RHV13_002471"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HDV1241996.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18710..20470)
                     /locus_tag="RHV13_002472"
     CDS             complement(18710..20470)
                     /locus_tag="RHV13_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HDV1241997.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEEYEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIVSLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20656..21108
                     /gene="matP"
                     /locus_tag="RHV13_002473"
     CDS             20656..21108
                     /gene="matP"
                     /locus_tag="RHV13_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HDV1241998.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(21185..22237)
                     /gene="ompA"
                     /locus_tag="RHV13_002474"
     CDS             complement(21185..22237)
                     /gene="ompA"
                     /locus_tag="RHV13_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HDV1241999.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKH
                     FTLKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQG
                     LSERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRV
                     EIEVKGIKDVVTQPQA"
BASE COUNT         5777 a         5296 c         5913 g         5560 t
ORIGIN      
        1 attatcatat ttacgaagag atcaggatga taacgatgaa aaaaagtgta ttgacggcgt
       61 ttataactgt ggtatgtgca acgtccagcg ttatggctgc tgatgataat gctatcacgg
      121 atggctcagt aacatttaat ggtaaagtta ttgctccagc ttgtaccctg gtagctgcga
      181 cgaaagattc agtagtcaca ttgccaaatg tcagcgccac caagttgcaa actaatggag
      241 cagtctctgg cgttaaaact gatgtaccaa ttgccttaga aggctgtgat gttactgtaa
      301 ctaaaaatgc gacgtttacc tttagtggta cggctgacgg cgtgcaacct acagcatttg
      361 ccaaccaggc taccaccgat gcagcgacga atgttgctct acaaatgtat ctgccagatg
      421 gttctacatc ggtaactccc ggtaccgaaa cgtcgaatat tcagttagcg gattctgctg
      481 agcaaacagt gacctttaaa gttgattata tcgctacggg gaaagcgact tctggtaatg
      541 tgaatgcggt aacaaatttc catattaact attattaata gaactcatta attgttttat
      601 taattagtac ccctctagtg ttctggaggg gatattcata ttttttaaga gtgactattt
      661 atgaaaactt gcataacaaa gggagttgtg accgtaagtt taacggcaat attactcagc
      721 tgttcgtcca catgggcggc aggtaaaggc ggggttggac ttgcagcaac acgtcttgtt
      781 tattcggaag gtgaagagca aatttcactg ggtgtacgta acaccagtcc ggatgttcct
      841 tatctgattc agtcatgggt gatgacacca gataataaaa aatcagcgga ctttattatt
      901 acaccaccgt tatttgtgct gaatccggca aatgagaatc tgttacgcat tatgtacatt
      961 ggtgcgccgt tggcgaaaga cagagaaacc cttttcttca ctagcgtacg ggcagtccct
     1021 tcaacaacga agcgggaaga gggaaatacc ctgaagattg ccacacaaag cgtcatcaaa
     1081 cttttctggc gaccaaaagg tttagcgtat cccttaggcg aggctccggc gaaactgcgt
     1141 tgcacttcgt cagctgacat ggttacggtc agtaacccaa caccttattt cattaccctg
     1201 acagacctga aaataggtgg aaaagtagtt aaaaatcaaa tgatttcccc ctttgataaa
     1261 taccaattct ctctgccaaa gggggccaaa aatagcagcg taacgtatcg aaccatcaat
     1321 gactacgggg cggaaacgcc gcaactcaac tgtaaatcgt aagccgtctt cagttaagag
     1381 agcgagatgt atagaactca tcgacaacac agcctgttaa gctctggtgg agtgccatcg
     1441 tttattggtg ggctggtggt gtttgtgtcg gcagcgttca atgcacaagc tgaaacctgg
     1501 ttcgatcctg cttttttcaa agatgatccc tcaatggtgg ccgatttgtc tcgtttcgaa
     1561 aaaggacaaa aaataacgcc aggggtttat cgtgtcgata tcgttctgaa tcagacaatt
     1621 gtagatacgc gcaacgtcaa ttttgttgag ataacgccag agaaggggat tgccgcctgt
     1681 ttgacgactg aaagcctgga tgcaatgggt gttaatactg atgcgtttcc ggcttttaaa
     1741 caactggaca aacaagcgtg tgtgccattg gcggagatta ttccggatgc cagcgtaacg
     1801 tttaatgtga ataaactccg tctggaaatt tcagtaccgc aaatcgccat caaaagtaat
     1861 gcccgtggtt atgtcccccc tgaacgttgg gatgaaggga tcaacgcgct attactggga
     1921 tattcgttta gcggggctaa cagtattcat agcagcgcag acagtgattc tggcgacagc
     1981 tattttctga atttaaacag tggcgttaat ttaggcccat ggagattgcg caacaattca
     2041 acatggagtc gcagtagtgg ccaaaccgca gaatggaaga atctcagcag ctatttgcag
     2101 cgggcggtta ttccactaaa aggcgaactg accgtaggtg atgattatac tgcaggcgat
     2161 tttttcgata gtgtcagctt tcgtggtgtg cagctggcgt cagatgacaa catgctgcca
     2221 gacagcctga aagggtttgc gcctgtggtg cgtggtatcg ccaaaagcaa tgcccagata
     2281 acgattaagc aaaatggtta caccatttac caaacttatg tatcgcctgg tgcttttgaa
     2341 attagtgatc tctattccac gtcgtcgagc ggtgatttgt tagttgaaat caaagaagcg
     2401 gacggcagcg tcaatagcta cagcgtaccg ttttccagcg tgccattact ccagcgtcag
     2461 gggcgaatca aatacgcggt gacactggcg aaatacagaa ccaatagtaa tgaacagcag
     2521 gagagcaaat ttgcccaggc cacgttgcag tggggcggac cgtggggaac gacatggtat
     2581 ggtggtggac aatatgctga atattaccgt gccgccatgt ttggtctggg atttaacctt
     2641 ggcgatttcg gagcaatttc gttcgatgcg acccaggcga agagtacgct ggcagaccaa
     2701 agcgaacata aaggtcagtc atatcgtttt ctgtatgcca aaacgctcaa ccaattgggc
     2761 actaattttc aattgatggg ctatcgctat tcgacgtcgg gtttctacac cctttccgac
     2821 accatgtata aacatatgga tggctacgaa tttaatgacg gtgatgatga agatacgccg
     2881 atgtggtcgc gttattacaa tttgttttac accaaacgtg gcaaactgca ggtcaatatc
     2941 tcccagcaat taggcgagta cggttcgttt tatttaagtg gtagccagca aacttactgg
     3001 cataccgatc aacaggatcg gctattacag tttggctaca acacgcaaat taaagatctc
     3061 tcgctggggg tttcctggaa ctacagtaag tcccgtggtc aacctgatgc tgatcaggtg
     3121 tttgcactaa atttttccct gccgctcaat ctgttgctcc ccagaagtaa tgatagctat
     3181 accaggaaaa aaaattacgc ctggatgacc tctaacacca gtatcgataa cgaagggcac
     3241 attacacaaa acctgggttt aacggagaca ctactcgatg acggtaacct gagctacagc
     3301 gtgcaacagg gatataacag cgaggggaaa acggctaatg gtagcgccag catggactac
     3361 aaaggggcgt ttgcagatgc ccgagtgggc tacaactaca gcgataacgg cagtcaacaa
     3421 caactgaact acgctctttc aggcagttta gttgcccatt cacatggcat taccctgggg
     3481 caatcgctgg gggaaactaa cgtgctgatt gcagcaccag gcgcagagaa tactcgtgtg
     3541 gcgaacagca ccgggctgaa aactgactgg cgcggatata ccgttgtccc ctatgccact
     3601 tcttatcggg aaaatcgaat cgcacttgat gcggcatcgt taaaacgtaa cgtggatctt
     3661 gaaaatgcag tagtcaacgt ggttcccacc aaaggggcgt tggttctggc ggagttcaat
     3721 gcccatgcgg gagcaagggt attaatgaaa acatcaaagc agggtatacc gctgcgtttt
     3781 ggcgcgatag cgacgctgga cggcatacag actaatagcg gcattattga tgatgatggc
     3841 tcgctctata tgtccggttt gcccgctcag ggagcgataa ctgtacgctg gggcgaagct
     3901 cccgatcaaa tttgtcatat cagttaccag cttaccgaac aacaaattaa ctctgcgatt
     3961 acgcgaatgg atgccatatg cagataatct ttggagaaaa atgcgtgtta ttactacgac
     4021 tattttttgc cgccgtctta atgctatggt gcgctcaaac tgctgcttat agcgggcagt
     4081 gtcataccac tcaggggaat ccgtatattg gcgtcaattt tggcgttaaa actctggagg
     4141 aagaagaaaa tacggctgga gtagttaaag acaaatttta tcagtggaac gaatcgaatg
     4201 attattatgt ttcctgtgat tgcgataaag acaatgtcag aagtggccga tgggcattcg
     4261 ccgcggattc accgttagtc tatttaggcg acaactggta caaaattaat gactatcttg
     4321 ccgccaaagt tttattgcag gttaaaggca gttctcctac tgcggttcct ttcgaaaacg
     4381 tgggcacagg ggcagataca cgatggcata tttgcgatcc cggcggtcaa cgtttaggtg
     4441 gccagggggc tagcggtaat agcggtagct tttccctgaa aatattgcag ccgttcgttg
     4501 gttcggtcgt cattcctcct atggcgctgg cgcgattatt tgaatgctac aacatacccg
     4561 caggtgattc ctgcacgact acaggtacac cggttttagt gtattacctg tctggtacta
     4621 tcaattcact tggctcatgt tccgtcaatg ccggagaaac aatcgaggtc gatctgggcg
     4681 acgtatttgc ggctaacttt cgtgttgtag ggcataagcc tcttggggcc agaacggcag
     4741 aacttgcaat tccagtcagg tgtaacacgg gaaacgcggg gttagttaac gtcaacctga
     4801 gtttgacggc aaccacagac cccagctatc cccaggcgat taagacgtca cgtcctggcg
     4861 tgggcgtggt ggtgaccgat agccagaaca acattatttc ccctgctggt ggaacattac
     4921 cgctctctat tcctgatgat gcagacagta tcgcgcgaat gaatgtctat ccagtcagca
     4981 cgacaggtgt accaccagaa accgggcgat ttgaagccac ggcaacggtg agaataaatt
     5041 ttgattaacg cgtgaacgta tgaagaaaaa aacgatattt cagtgcgtta tcttgttctt
     5101 tagcattctt aacatccatg tcgggatggc tgggcctgaa caagttagta tgcatattta
     5161 tgggaatgtg gtcgatcagg gctgtgatgt cgccaccaaa agtgcattac aaaatattca
     5221 tattggtgat tttaatatca gtgattttca ggctgcgaat accgtaagca ctgctgctga
     5281 tttgaatatc gatatcaccg gttgtgccgc tggtattact ggcgcggacg tcctttttag
     5341 cggcgaggct gacacccttg cgccgacact gctcaaacta actgacacag gcggaagcgg
     5401 tggtatggca acggggattg ccgtgcaaat tcttgatgcg caaagtcagc aagaaatccc
     5461 gctcaatcag gtccagcctc ttacgccctt aaaagccggg gataacacac tcaaatatca
     5521 acttcgttat aagtccacaa aggcgggagc aacgggcggt aatgcgacgg cggttctcta
     5581 ttttgatctg gtttaccagt gaaggagtga tgttgaaacg gataatctgg attctgttct
     5641 tactgggatt aacggggggc tgtgagctat ttgcccatga tggcacggtc aacattagcg
     5701 gatcgtttcg ccgtaataca tgcgtgctgg cacaggatag caagcaaatc aatgtgcagt
     5761 taggcgatgt ctcgctgaca cgtttttttc atgggaatta tggcccggag aaatctttca
     5821 tcattaattt gcaggattgc ggaacagatg tcagcacggt tgacgtcacc ttttcaggaa
     5881 cccccgacgg agtgcagagc gaaatgctgt ccattgaaag tggtacggat gcagccagtg
     5941 ggctggcaat tgcgattctg gatgatgcga agatattgat tccgcttaac caggcgagta
     6001 aagactacag cctacatagc ggtaaggtgc tattaacttt ttatgcgcaa ttgcgacctg
     6061 tcaatagcga tgtgcagtcg ggtaaagtga atgccagcgc gacatttgta cttcattatg
     6121 actaatacgt ggaacagatt agcgctcttg attttcgccg ttttatcgct gctggtggcg
     6181 ggtgaattgc aggcaggagt ggtggtcggc ggaacgcgat ttatctttcc ggcagacaga
     6241 gaatcgatat ctattttact gactaatacc agtcaggaat cctggcttat taacagcaaa
     6301 atcaatcgcc caacgcgttg ggccgggggg gaagtgtcga cagtgccagc actattactg
     6361 gccgctccgc cacttattct cctgaagccc ggtactacag gcacgttgcg cttgctgaga
     6421 acggaaagcg acatcttgcc tgtggatcgc gaaacgctat ttgagttaag cattgccagc
     6481 gtgccatccg gcaaagttga aaatcagagc gtaaaagtgg cgatgcgctc ggtatttaaa
     6541 ctgttctggc gacccgaagg tttgccgggc gacccgctgg aagcttatca acaattacgc
     6601 tggacacgga attcgcaggg tgtacaactc actaacccaa cgccttacta cattaacctg
     6661 attcaggtga gcgtaaatgg taaagcgtta agtaatgcgg gagttgtgcc gcctaaaagc
     6721 cagcgtcaga caagctggtg tcaggcaatc gcaccgtgtc atgtcgcctg gcgggcgata
     6781 aatgattacg gcgggttgag tgcaaagaag gagcaaaatc tgccctgaaa caggttcgga
     6841 aaacgtttgc gttttttttg ccgcaggtca attccctttt ggtccgaact cgcacataat
     6901 acgcccccgg tttgcacacc gggaatccag gagagttcat gtactacccc ttcgttcgta
     6961 aagccctttt ccagctcgat ccagagcgcg ctcatgagtt tacttttcag caattacgcc
     7021 gtattacagg aacgccgttt gaagcactgg tgcggcagaa agtgcctgcg aaacctgtta
     7081 actgcatggg cctgacgttt aaaaatccgc ttggtctggc agccggtctt gataaagacg
     7141 gggagtgcat tgacgcgtta ggcgcgatgg gatttggatc gatcgagatc ggtaccgtca
     7201 cgccacgtcc acagccaggt aatgacaagc cgcgtctctt tcgtctggta gatgccgaag
     7261 gtttgatcaa ccgtatgggc tttaataatc ttggcgttga taacctcgta gagaacgtaa
     7321 aaaaggccca ttatgacggc gtcctgggta ttaacatcgg caaaaataaa gatacgccag
     7381 tggagcaggg caaagatgac tatctgattt gtatggaaaa aatctatgcc tatgcgggat
     7441 atatcgccat caatatttca tcgccgaata ccccaggatt acgcacgctg caatatggtg
     7501 aagcgctgga tgatctctta accgcgatta aaaataagca aaatgatttg caagcgatgc
     7561 accataaata tgtgccgatc gcagtgaaga tcgcgccgga tctttctgaa gaagaattga
     7621 tccaggttgc cgatagttta gttcgccata atattgatgg cgttattgca accaatacca
     7681 cactcgatcg ttctctggtt cagggaatga aaaattgcga tcaaaccggt ggcttaagtg
     7741 gtcgtccgct tcagttaaaa agcaccgaaa ttattcgccg cttgtcactg gaattaaacg
     7801 gtcgcttacc gatcatcggt gttggcggca tcgactcggt tatcgctgcg cgtgaaaaga
     7861 ttgctgcggg tgcctcactg gtgcaaattt attctggttt tatttttaaa ggtccgccgc
     7921 tgattaaaga aatcgttacc catatctaat tatttcttcg aattcctctt tataagataa
     7981 tcagggcttt attttcagcc ctggttgttt tatattcatc gctgttgctt atttagacat
     8041 tttgtacttt tattgttgag gttattaagc gaagcgacaa tggattgtgt tgctgcggtt
     8101 ttatagggta ggggagaggc agatgcgaat taaaccagac gataactggc gttggtatta
     8161 cgatgaagag cacgatcgaa tgatgctcga tttagccaat ggtatgctat ttcgctcacg
     8221 ttttgcgcgc aagatgttga ctccagatgc cttttctccg gcaggctttt gcgttgacga
     8281 cgccgcgctc tatttctctt ttgaagaaaa gtgccgcgat tttaatttat ctaaagaaca
     8341 aaaagccgaa ctggtgctga atgcactggt agcaattcgc tatctcaaac cgcaaatgcc
     8401 gaaaagctgg cattttgttg cccatggtga aatgtgggtg cccatgccgg gcgatgcggc
     8461 ctgcgtttgg ttgagcgata ctcacgagca ggtcaatttg ctggtcgttg aatctggcga
     8521 aaatgccgca ctatgcctgc tggcacaacc ctgcgttgtt attgcgggtc gcgcgatgca
     8581 gttgggcgac gccattaaaa tcatgaacga caggctgaaa ccgcaggtta atgttgacag
     8641 cttcagcctc gaacaggcag tctaacgcgc caacttaagt gcagtcttcg gtacacagct
     8701 acagcaaaga atggtgccat catcgcccat tgctgatttt ttcagcggcg tgacttcgcc
     8761 ttctaaaagc tgaacacggc aacttccaca aatgcccgcg cggcaagaat aagggatacg
     8821 aattccctga ttttctaatt gctccagcaa cacttgttgg ttatttccac gaaatgcctg
     8881 tccctgccag tcaatatcta catttgcgtc cggttgttgc gtgatgtggg cggtatcatc
     8941 agcggcagct gcgccgtaaa tttttgccgg agccgttgcc agaatttcca cctcatcgcc
     9001 aacgcgaatc acgccgctat tacgggcaat taaattctga ccaaaatcga catcgccgtt
     9061 atcctgggca gtgcggaaag attgcaatgt ttttaatggt tcgcctgccg gatgtttttg
     9121 ccctttttct gggctgacgg tggtgaaaat acagcggcta caaggtttaa ccacatcaaa
     9181 caccacatca ccaatgcgaa tcactttcca gcgatcttct tcccaggctg acgcgccgga
     9241 aaccaccaga ttggggcgga attgctccat ttttacactg gccggacaac gttgttggag
     9301 atcacgtaac gaggcttcgt tagcaagaag gtaaggatag ccatcagcaa atgacagagg
     9361 tacagtgttg tggcgtttca cgcgccgggt catttgtggc cccacccagc gtaattgcac
     9421 ttcgcgggag aaaaatccac ttagccattt gttgatcgcg tctggcgcaa ttcgcgcggt
     9481 aaaatgtgtg ccccaaactt cggttggtgc gtcttgtgtg gcgaaatcag caaaacgaac
     9541 atatgcgcta ctgccatctg gtgcggtgag atgcaagcca tcatgcacag gtgaaggagt
     9601 aaaccgtacc atctggggaa actggcgagc ggtaataaac gtaccgtcag gttccgtgat
     9661 cataaagatg cgatcgaagg ccagaccact gacatcagcc agagcatgtg taagaccaat
     9721 gccgcgcatc gatttaacag gatgaataaa aagccgggtt aatgtcgcca cggtgcggtc
     9781 ctcaaatgaa aataagccct caactttatg acatgacgcg cttattagct ataatgcgca
     9841 acaattttct tagcgcctgt cccattaggc tattttattt gtcagtttgg ccctgggttg
     9901 tgctcgaaat cctcacgtac ttgtgtacgc tccggtttct ccgcgctgtc catgtccaga
     9961 ctgactgcaa caattacgcc tactgcggta ggctcttaga gtaaaagtga cgatatgaat
    10021 tctctgtttg ccagtacggc ccgtgggctg gaagagctgt taaaaactga actggaaaac
    10081 ctgggggccg ttgaatgcca ggtggttcag ggtggggtcc atttcaaggg cgacacacgg
    10141 cttgtttacc agagcctgat gtggagccgc ctggcctcgc gtattatgtt gccgctgggc
    10201 gagtgtaagg tttacagcga tttagacctc tatctcggtg ttcaggcgat caactggaca
    10261 gagatgttta atcctggcgc gaccttcgct gtccacttca gtggtttgaa tgacaccatc
    10321 cgcaacagtc agtacggcgc gatgaaagtg aaagacgcga tcgtcgatgc tttcacgcgg
    10381 aaaaatctgc cgcgtccaaa tgttgatcgc gatgcgccgg atatccgcgt taacgtctgg
    10441 ctgcataaag aaaccgccag tatcgctctt gatctgagtg gtgatggttt acatctgcgt
    10501 ggctatcgcg atcgtgctgg tattgcgccg atcaaagaaa ccctggcagc cgctattgtg
    10561 atgcgatccg gctggcagcc aggaacaccg ctgctcgatc cgatgtgtgg ttccggtacg
    10621 ttgctgattg aagcggcgat gctggcgacc gatcgcgcac caggcttgca ccgtgggcgt
    10681 tggggcttta gcggctgggc gcagcatgat gaagctatct ggcaggaagt gaaagcggaa
    10741 gcgcaaactc gcgcccgtaa aggcctggct gagtatagct ctcattttta cggttcggac
    10801 agcgacgcac gggtgattca acgtgcacgc actaacgccc gtcttgcggg gattggtgaa
    10861 ctgatcacct ttgaggtgaa agatgtcgcg caactgacca atccgctgcc gaaagggccg
    10921 tacggcacag tgttgagcaa cccgccatac ggggaacgtc tggacagcga accggcgctg
    10981 attgcgctgc atagcctgct gggtcggatc atgaaaaacc agtttggtgg ctggaatctc
    11041 tctttgttta gtgcctcgcc ggatctgcta agctgcttgc agctgcgtgc agacaaacag
    11101 tacaaggcga aaaacggccc gctggactgc gtacagaaaa attaccacgt tgccgaaagc
    11161 actccagaca gcaaaccggc gatggtagcg gaagactacg ccaaccgcct gcgtaagaac
    11221 ctcaaaaaat tcgagaagtg ggctcgccag gaaggaattg aatgttaccg cctgtatgac
    11281 gccgatctac cggaatataa cgttgccgtt gaccgttatg ccgactgggt ggtggtgcag
    11341 gagtatgcgc cgccaaaaac tattgatgct cacaaagcgc gtcagcgtct gttcgatatt
    11401 atcgctgcaa ccatttcggt actggggatt gcgccaaaca aactggtgct gaaaacccgt
    11461 gaacgccaga agggcaaaaa tcagtaccag aaactgggcg agaagggcga gtttcttgaa
    11521 gttaccgaat ataacgctca cttgttggtg aacctgacgg attacctcga taccggtctg
    11581 tttctcgatc accgtatcgc ccgtcgtatg ctcggtcaga tgagcaaagg caaagatttc
    11641 ctcaacctgt tctcttatac cggcagcgcc accgtgcatg cgggattagg cggtgcacgc
    11701 agcaccacca ccgtggatat gtcgcgtact tatctggagt gggcagaacg caacctgcgt
    11761 ttgaatggcc tgaccgggcg tgcgcatcgc ctgattcagg ccgattgcct ggcgtggctg
    11821 cgtgaggcaa atgaacagtt cgatctgatc tttatcgatc cgccaacctt ctctaactca
    11881 aaacgaatgg aagatgcgtt tgatgttcag cgcgatcatc tggcgctgat gaaagatttg
    11941 aaacgtctgc tgcgtgcagg tgggacgatc atgttctcga acaataaacg tggcttccgt
    12001 atggatctcg acggcctggc aaaactggga ctgaaagcac aagaaattac gcaaaaaacg
    12061 ctctcccagg atttcgcccg taaccgccag atccacaact gctggctgat taccgcagcc
    12121 tgaaaggaat agtaatgtca ttaatcagta tgcatggcgc atggctgtcg ttcagcgacg
    12181 cgccgcttct cgataacgca gaactgcata tcgaagataa cgaacgtgtt tgtctggtgg
    12241 gccgcaacgg cgcaggcaaa tcgacgttaa tgaaaatcct caaccgtgaa caagggctgg
    12301 atgacggtcg cattatttac gagcaggatt tgattgtagc gcgtctgcaa caggatccgc
    12361 cgcgtaacgt tgagggtagt gtttatgatt tcgttgccga aggcattgaa gaacaagcgg
    12421 aatacctgaa acgctatcac gatatttcgc gccaggtgat gaacgacccg agcgagaaaa
    12481 atctcaacga actggcgaag gttcaggaac agctggatca ccacaacctg tggcagctgg
    12541 aaaaccgcat caacgaagtg ctggcgcaac tggggttaga tcctaacgtt gcgctgtcgt
    12601 cgctttccgg cggctggttg cgtaaagcgg cattaggacg cgcgctggtg agcaatccgc
    12661 gcgtattgct gctggacgaa ccgactaacc acctggatat cgaaaccatc gactggctgg
    12721 aagggttcct caaaaccttc aatgggacga ttattttcat ctcccacgac cgttcgttta
    12781 tccgcaatat ggcgacgcgc attgttgatc tcgatcgcgg caagctggtg acctatccgg
    12841 gcaattacga tcagtacctg ctggaaaaag aagaagccct gcgcgtggaa gaactacaaa
    12901 atgccgagtt cgatcgcaaa ctggcgcagg aagaggtgtg gatccgccag gggatcaaag
    12961 cacgccgtac ccgtaatgaa ggccgcgtac gtgccctgaa agcgatgcgg cgcgagcgtg
    13021 gcgaacgtcg cgaagtgatg ggtaccgcaa agatgcaggt ggaagaggcc agccgctccg
    13081 gtaagatcgt tttcgaaatg gaagacgttt gctaccaggt taacggtaag caactggtga
    13141 aagatttttc tgcccaggtt ctacgtggcg acaaaattgc cctgattggt ccgaatgggt
    13201 gcggcaaaac cacgctgctg aaactgatgc tcggtcagct tcaagcggac agcgggcgta
    13261 ttcacgttgg caccaaactg gaagtggctt atttcgatca gcaccgcgcg gaactggatc
    13321 ccgataaaac ggtgatggat aaccttgccg aaggtaagca agaggtgatg gttaacggca
    13381 agccacgcca cgttttgggc tatttgcagg acttcctgtt ccatccgaaa cgggcgatga
    13441 cgccagtacg tgcgctttct ggcggtgagc ggaaccgctt gctgctggcg cgtttgttcc
    13501 tcaaaccaag caacttattg attcttgacg aaccgaccaa cgatcttgat gtcgaaacgc
    13561 tggaactgct ggaagaactg atcgacagct accagggcac ggtattgctg gtaagccacg
    13621 atcgtcagtt tgtcgataac accgttacag aatgctggat cttcgaaggc ggcggtaaaa
    13681 ttggtcgtta tgtcggcggt tatcatgatg cccgtggtca gcaagaacag tatgtggcgc
    13741 ttaaacaacc tgcggtgaaa aaaaacgaag aagccgctgc gccaaaagca gaaactgtaa
    13801 aacgcagcag tagcaaacta agctataaat tgcagcgcga actggagcag ctaccgcaat
    13861 tgctcgaaga tctggaggcg aagctggaag ccctacagac gcaggtggcg gatgcttcct
    13921 tcttcagtca gccgcatgag cagacgcaaa aagtgctcgc tgatatggct gccgcagaac
    13981 aggaactgga gcaagccttt gaacgctggg agtatcttga agcgttaaaa aatggtggct
    14041 gatcgcaaaa tagtcgattt aaacagagac agctgacaac ggtagaattg cctgatgaac
    14101 tgggtttatc aggcatgaaa accaacactt tgttagtcat ctgtaaaata gcgcatcatt
    14161 aaggagtacc aatgtgcgaa catcatcatg ccgcgaagca catcctgtgc tcgcagtgtg
    14221 acatgctggt ggcgttaccg cgccttgagc atggtcagaa agcggcatgt ccccggtgtg
    14281 gcacaacgtt aaccgtggcg tgggatgccc ctcggcagcg tcctaccgcc tatgcgttgg
    14341 ctgcactgtt catgctgttg ctgtccaact tgtttccttt tgtgaatatg aacgttgcag
    14401 gagttaccag tgaaattaca ttactggaaa ttcccggcgt gcttttttct gaggactacg
    14461 ccagcctcgg cacctttttc ctgttgtttg tgcaactggt tcccgcgttt tgtctgataa
    14521 ccattctgtt actggtgaat cgcgcggaat taccggtccg tttaaaagag cagctggcac
    14581 gggtgctttt tcaactcaaa acctggggaa tggcggagat tttcctcgcg ggtgtgctgg
    14641 tcagtttcgt taaactgatg gcttacggca gcattggtgt aggcagcagc tttctcccat
    14701 ggtgtttatt ttgtgtcctg caactgcgag cttttcagtg cgttgatcgt cgctggttat
    14761 gggacgatat cgccccgatg ccagaactgc gccagccgct aaaaccaggc gtcacgggga
    14821 tacgtcaggg gctgcgttcg tgctcctgtt gtacggcaat ccttcctgct gatgaacccg
    14881 tgtgcccgcg ctgtggtact aaagggtacg ttcgacgtag aaacagcctg caatggacac
    14941 tcgcgctgct tgttacgtcc atcatgctgt atcttccggc taatattttg cccatcatgg
    15001 tgacggattt attaggctcg aagatgccat cgacgattct cgctggggtc atcctgttat
    15061 ggagcgaagg ctcttatccc gtcgctgcgg tgatcttcct tgccagtatt atggtgccaa
    15121 cgttaaagat gatcgccatc gcgtggctgt gttgggatgc caaagggcat ggcaagcgcg
    15181 acagtgaaag aatgcatttg atttatgaag ttgttgagtt tgtaggccgc tggtcgatga
    15241 ttgacgtttt cgttatcgcg gtgctctcgg cgctggtgcg tatgggaggt ttaatgagta
    15301 tttatccggc aatgggtgca ttaatgtttg ctttagtcgt cataatgaca atgttttctg
    15361 ctatgacgtt tgacccgcgt ttgtcgtggg atcgtcaacc tgaatcagag catgaggagt
    15421 cctgacagta tggaatctaa taatggggaa gccaaaatcc agaaagtgaa gaactggtct
    15481 cccgtgtgga tatttcctat cgtcacggcg ctcattgggg cctgggttct tttttatcat
    15541 tacagccatc agggaccgga agtgaccttg atcaccgcga atgcggaagg aattgaaggt
    15601 ggcaaaacca ccattaaaag ccgtagcgtt gacgtcggcg tggttgaaag cgccacactc
    15661 gctgatgatt tgacacacgt tgaaatcaaa gcgcggctga attccggtat ggaaaaattg
    15721 ctgcataaag ataccgtctt ttgggtggtg aaaccgcaga ttggtcgaga agggattagc
    15781 ggtctgggaa ccctgctgtc tggggtttat atcgaactgc agccaggcgc gaaaggcagc
    15841 aaaatggata aatacgattt gctggactcg ccaccgttgg ccccgcctga tgcgaaaggt
    15901 atccgtgtga ttctcgatag caaaaaagcc gggcagctct cgccaggaga tccggtgctg
    15961 ttccgtggct atcgggtagg ttcggttgaa accagcacct tcgatacgca aaaacgcaat
    16021 atcagttatc aactgttcat caatgcacct tatgaccgac tggtgaccag caatgttcgc
    16081 ttctggaaag atagtggcat tgcggttgat ctgacgtcag cgggaatgcg tgtggagatg
    16141 ggctcattga caacgctgct gagtggcggt gtcagctttg atgtgccgga aggtctggat
    16201 ttagggcagc cagtggcacc gaaaacagct ttcgttttgt atgatgatca gaagagcatt
    16261 caggattcgt tgtacaccga tcacattgat tatctgatgt tctttaaaga ttcggtacgc
    16321 ggtctgcaac cgggagctcc ggtagaattc cggggtattc gcctgggtac cgtaagcaaa
    16381 gtgccattct ttgcgtcgaa tatgcgtcag acatttaacg atgattaccg tattccggtg
    16441 ctgattcgta tcgagccaga gcggctgaaa atgcagcttg gcgaaaatgc ggatgttgtt
    16501 gagcaccttg gcgaattgtt gaaacgtggt ttacgcggat cgctgaaaac cggaaacctg
    16561 gtcactggcg cactgtatgt tgatctcgat ttctatccaa atacgcctgc aataaccggt
    16621 attcgtgaat ttaatggtta tcagattatc ccgaccgtta gcggcggcct ggcgcaaatc
    16681 cagcaacgac tgatggaagc gttggataag atcaacaaac tgccattgaa tccgatgatt
    16741 gaacaggcaa ccagtacgct ttctgaaagt cagcgcacaa tgaaaaacct gcaaacgacg
    16801 ctggatagca tgaacaagat cctcgccagc cagtcgatgc agcagttgcc gacggatatg
    16861 cagtcaacgt tgcgtgaatt gaatcgcagc atgcagggct tccagcctgg ctccgcagcc
    16921 tacaacaaga tggtggcgga tatgcagcgc cttgatcagg tgttgcgaga actgcaaccg
    16981 gtgctgaaaa cgctcaatga gaagagtaac gcgctggtat ttgaagcgaa ggacaaaaaa
    17041 gatccagagc cgaagagggc gaaacaatga aaaagtggct agtgacgatt gcagcactgt
    17101 ggctggccgg atgcagctcc ggcgaaatta ataaaaacta ttaccagtta cctgtggtgc
    17161 agagcggtac acaaagtacc gccagccagg gcaatcgcct gttatgggta gagcaggtcg
    17221 ctgtacctga ctatctggcg gggaatggtg tggtttatca aaccagtgat gtgaagtatg
    17281 tgattgccaa caacaacttg tgggccagcc cgttggatca acagttgcgc aacaccctgg
    17341 tagccaacct gagcacgcaa ctgcccggct gggtggttgc ctcccagcct ctgggaagcg
    17401 cccaggacac gctcaatgtt accgtaacgg agtttaacgg tcgctatgat ggcaaggtca
    17461 ttgtcagtgg tgagtggctg ttgaaccacc agggacaact gatcaaacgt ccgttccgtc
    17521 tggaaggagt gcaaactcag gatggttacg atgagatggt taaagtgctg gccggtgtct
    17581 ggagtcagga agccgcttct attgcacaag agataaagcg tctaccttaa ttataaagat
    17641 ttgtaaatat aaccgtctcc ggtatgttgc ctgaggcggt ttttttgttt ctaacgtgcg
    17701 gaaaaatttg ttcctcttca cattttttgt acaaccgaca tgcccgtgta gctcacaaat
    17761 atgacagtgg cgtgaatttt gcgcattgac ggcagttatg attcgcggta ttgcttaact
    17821 gtgattgcac atttagtaat cactgttttc ttttccacca gaaaccagta tgagggaaac
    17881 gaggcatgaa gagacaaaaa cgagatcgcc tggaacgggc acatcaacgt ggttatcagg
    17941 ccggcatcgc cggacgctca aaagaaatgt gtccctatca gacgctgaat caaaggtcac
    18001 aatggctggg aggctggcga gaagccatgg cggacagggt agtaatggcc tgattctgtc
    18061 tctttaaaaa gaaacctccg cattgcggag gtttcgcctt ttgatactct gtctgatgat
    18121 aatcagaagg cagacgtatc ctggaacaga ccgactttca ggtcgctggc ggtatagatc
    18181 agacgaccat caaccagcac ttcgccatct gccagaccca taatcagacg acgattaaca
    18241 atgcgtttaa agtgaatacg gtaggtcact tttttcgctg tcggcagtac ctgaccagtg
    18301 aatttcactt cgccaacgcc cagcgcgcgg cctttacctt cgccgcccag ccagccgagg
    18361 tagaacccta ccagctgcca cattgcgtcc aagcccaggc atcccggcat aaccggatcg
    18421 ccaatgaagt ggcatccgaa gaaccacaga tccggattga tatccagttc tgcttcaaca
    18481 taccctttgt cgaagttacc acccgtttcg gtcattttga ccacacggtc catcattagc
    18541 atgttcggtg ctggcaattg cgggccttta gcgccaaaca gttcaccgcg accagaggca
    18601 agaaggtctt cttttgtata ggattcgcgt ttatctacca tgttctctgt aagccttatt
    18661 ttattgaagc acgcaggata gctaacacgt gtacgctgaa caagtccgat cagttcggaa
    18721 taaaccagtt cagccaacgt aatggccatg gaaaacggtg acgtccttcc tgttgcgatg
    18781 cttgcgcgat acgttcctgg atggtttgca tcagcgtcgt ttggccttcg ccatcccaca
    18841 ccagatttaa taataacggc agtgcgtcag tcacatcgtc tactgcccag atggtgaatt
    18901 tgccttcttc taccgctttc accagttcac tgtgaagact taaatggcga acgttagctg
    18961 tggggataat gacaccttgt ttacccgtta actcacgttg ctggcaaata gcaaagaagc
    19021 cttcgatttt ctcatttaaa ccaccgaccg gctgggcgcg accgaactga tcgactgaac
    19081 ctgtgatagc gatactctga ttcaccggca catcagcgag ggcgcttatc agggcgcaga
    19141 gttcagccat cgaggcacta tctccatcaa cttcactgta tgactgctca aatgtcagcg
    19201 atgctgagaa ggggatctgt tgctcaagct gtagttccga catcaggaac gcctgcatga
    19261 tcatcattcc tttcgcatgg atattgccgc caagctccgc tttgcgttcg atgtcggtga
    19321 attcaccatc gccaatatgc acaacgcagc taatgcgaga aggttcgcca aaagcgcgtg
    19381 gatgacccgg aaattcaatg accgaaaggg cgttaatttg cccgatgcgt tcgccttcgg
    19441 tttcaatcag gatttgctcc tgaaggatct catcctgcat acgttcagcg aggaaacctt
    19501 cgcgccattc acgctgctgc agcattaagt ttagctgctc gccggagaag gtgtcgccat
    19561 cacacaggga ggcgacctct ttacactggc ggaggatcca ctgcgggcta agcggaagtg
    19621 tttcttgttc accggtgtag cgtgctgctt cgcggataag taccggccag gcatccgctc
    19681 ccggggcagg taagtgatta tgtctggcgg taaatgtcac ccagcgacac cactggctta
    19741 ctgactccgc atcgacaatc tgcagagtat cttcaaattc gctataaata gcctgctctg
    19801 aaagctctgg ctccatctcc tggaaatcag ccaatgattc gcgttcgcct accagaatga
    19861 ctttcagctt caatggcatc gaaggcacag agacggggag agggcgcgac tcatcaaacg
    19921 caacccagtc aaaacgctcg cggttaacga tatttttcag ccgcatccac agcagaggtt
    19981 gcgccagcag tgtacgcaaa gagacgatga gaataccgcc atttgcctga tgcaccagac
    20041 caggctgcag ggtaatgtcg ccattaaact ggcgcaggca gccaaagagt tgctccgctt
    20101 ctacccagtc ggcagcgaca acttgcgtta aagtcgcaaa attatcatct gcactcactg
    20161 cgtgacgtaa gcggatggag tggccggaaa cctcatagtg atcgcccacc agttggcctg
    20221 catcgctttg tagcgtacgc gcggcatcgg caataagatt cagatactca tactcttccg
    20281 gggccttagc cagcataaaa gaggaggatg ctcgcgtatg cagcaactgc tccagcgcaa
    20341 attgcagtcg cggttgagta tcactgaata aaggatcgtt ttcgtcaatc aaatgtggct
    20401 gagcaaatat ttcctgatag ctatcggtat caggaaccag gtcacgccat gcaagtttcg
    20461 taatggtcaa agttgatgtt ttttagtctg ttgtcaaagc cgcgattata ccgtaaccgg
    20521 cactacagca cacgtagaaa gcaccgacaa tactcatggc atgggcgtta aagctcacag
    20581 gatggagatt cttttcttca ctggcctaaa aagctgatat tctgtaaaga gttacacggt
    20641 aacattgaga tcgctatgaa atatcaacaa cttgaaaatc ttgaaagcgg ttggaaatgg
    20701 aagtatctgg tgaagaagca tcgcgaaggg gagttaatta cccgttacat agaagccagt
    20761 gccgcccagg aagccgtcga tgtgttgctc tcgctcgaaa atgaacccgt gctggtaaat
    20821 ggctggattg acaagcatat gaatccggaa ctggtcaatc gcatgaagca aaccattcgg
    20881 gccagacgga agcgccattt taatgcggaa caccagcata cgcgcaaaaa gtcgatcgat
    20941 ctggaattta ttgtctggca acgtctggct ggtcttgcgc agcgtcgcgg taaaacgctt
    21001 tctgaaacga ttgttcagct gattgaagat gcggaaaaca aagagaaata cgcgaataaa
    21061 atgtcttctt tgaagcagga tctgcaggca ttgctgggta aggaataact gacgaaactc
    21121 agttcaattt actaaaggtg aaaaaaaaac cccgcagcag cggggttttt taccagacga
    21181 gaacttaagc ctgcggctga gttacaacgt ctttgatacc tttaacttcg atctctacgc
    21241 gacgatccgg agccaggcag tcgatcagtg cagcacgctg tttcacgttg tcgcaggtgt
    21301 tgccagtaac cgggttggat tcgcccatac cacgtgcgga gatcttgtct gccgggatac
    21361 ctttggagat caggtaatca acaacagact gagcacggcg ctcggacaga ccctggttgt
    21421 aagcgtcaga accgatgcgg tcagtgtaac ccagaacaac tacggaaccg tctttcggat
    21481 ccaggttgct cagctggctg tacagctgat ccagagcagc ctgaccttcc ggtttcaggg
    21541 ttgctttgtt gaagttgaac agaacgtcag acttcagagt gaagtgcttg gtctgtactt
    21601 ccggtgccgg agctggagcc ggagcaacta ccggagctgc ttcgccctga ccgaaacggt
    21661 aggaaacacc caggctcagc agaccgttgt ccggacgagt accgatggtg tttgcgtcac
    21721 cgatgttgtt ggtccactgg tattccagac gggtagcgat ttcaggagtg atcgcgtact
    21781 caacaccacc tgcgaaaacc ggagaaacgc cagtgtcgtg gtctttaaag gatgcgccac
    21841 caggtacgtt agccttggtg tctgcacgcc ataccatacc acccagacga gtgtagatgt
    21901 ccagatcgtc agtgattggg taacccagtt tagcggtcag ctgaacgccc tgagctttgt
    21961 atgcgccgtt gatgttgtcg cctttgtacg gcatacgacc taaccagtcg taacccattt
    22021 caaaaccaac atacgggtta acctggtaac caccaaaagc acctgcaccc agttggtttt
    22081 cgtgggtcgg accattgtta ggaataaaac cagtgtcatg gtactgggac cagcccagtt
    22141 tagcaccagt gtaccaggtg ttatctttcg gagcggcctg cgctacggta gcgaaaccag
    22201 ccagtgccac tgcaatcgcg atagctgtct ttttcatttt ttgcgcctcg ttatcatcca
    22261 aaatacgcca tgaatatctc caacgagata acacggttaa atccttcacc gggggatctg
    22321 ctcaatatta actctaccga tatcttcggc ttatgccgag cacccctggc gatgtaaagt
    22381 ctacaacgta gttgaaaact tacaagtgtg aactccgtca ggcatatgaa aaaaaagtct
    22441 tgtataaggt acgtttaatc ttttttgtca gcgacaattt acagaaaaaa aacgcggaaa
    22501 ccgcttcaga caagcctccg caaggaaaat tagtcacgac tgaaag
//
LOCUS       DAPGOF010000075        22502 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.075, whole genome shotgun sequence.
ACCESSION   DAPGOF010000075 DAPGOF010000000
VERSION     DAPGOF010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.075"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..159)
                     /locus_tag="RHV13_002475"
     CDS             complement(<1..159)
                     /locus_tag="RHV13_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDV1242000.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHSLTVDN"
     gene            complement(169..720)
                     /gene="ybcL"
                     /locus_tag="RHV13_002476"
     CDS             complement(169..720)
                     /gene="ybcL"
                     /locus_tag="RHV13_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HDV1242001.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEVK"
     gene            complement(889..1221)
                     /gene="emrE"
                     /locus_tag="RHV13_002477"
     CDS             complement(889..1221)
                     /gene="emrE"
                     /locus_tag="RHV13_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HDV1242002.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(1555..1869)
                     /gene="fliE"
                     /locus_tag="RHV13_002478"
     CDS             complement(1555..1869)
                     /gene="fliE"
                     /locus_tag="RHV13_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HDV1242003.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            2084..3742
                     /gene="fliF"
                     /locus_tag="RHV13_002479"
     CDS             2084..3742
                     /gene="fliF"
                     /locus_tag="RHV13_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HDV1242004.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVIVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESQAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            3735..4730
                     /gene="fliG"
                     /locus_tag="RHV13_002480"
     CDS             3735..4730
                     /gene="fliG"
                     /locus_tag="RHV13_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HDV1242005.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            4723..5409
                     /gene="fliH"
                     /locus_tag="RHV13_002481"
     CDS             4723..5409
                     /gene="fliH"
                     /locus_tag="RHV13_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HDV1242006.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            5409..6782
                     /gene="fliI"
                     /locus_tag="RHV13_002482"
     CDS             5409..6782
                     /gene="fliI"
                     /locus_tag="RHV13_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HDV1242007.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTTLISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            6801..7244
                     /gene="fliJ"
                     /locus_tag="RHV13_002483"
     CDS             6801..7244
                     /gene="fliJ"
                     /locus_tag="RHV13_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HDV1242008.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            7241..8368
                     /gene="fliK"
                     /locus_tag="RHV13_002484"
     CDS             7241..8368
                     /gene="fliK"
                     /locus_tag="RHV13_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HDV1242009.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPSAKGEPLVSEILADAQQADLLIPVDETPPVINDEQSTSTPLTTAQ
                     TMTLAAVAGNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPTEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDMLPVPVSLQGRVTGNSGVDIFA"
     gene            8473..8937
                     /gene="fliL"
                     /locus_tag="RHV13_002485"
     CDS             8473..8937
                     /gene="fliL"
                     /locus_tag="RHV13_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HDV1242010.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            8942..9946
                     /gene="fliM"
                     /locus_tag="RHV13_002486"
     CDS             8942..9946
                     /gene="fliM"
                     /locus_tag="RHV13_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HDV1242011.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            9943..10356
                     /gene="fliN"
                     /locus_tag="RHV13_002487"
     CDS             9943..10356
                     /gene="fliN"
                     /locus_tag="RHV13_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HDV1242012.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            10359..10724
                     /gene="fliO"
                     /locus_tag="RHV13_002488"
     CDS             10359..10724
                     /gene="fliO"
                     /locus_tag="RHV13_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HDV1242013.1"
                     /translation="MNNHATVQSSAPVSTAPLLQVSGALIAIIALILAAAWLIKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            10724..11461
                     /gene="fliP"
                     /locus_tag="RHV13_002489"
     CDS             10724..11461
                     /gene="fliP"
                     /locus_tag="RHV13_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HDV1242014.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            11471..11740
                     /gene="fliQ"
                     /locus_tag="RHV13_002490"
     CDS             11471..11740
                     /gene="fliQ"
                     /locus_tag="RHV13_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HDV1242015.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            11748..12533
                     /gene="fliR"
                     /locus_tag="RHV13_002491"
     CDS             11748..12533
                     /gene="fliR"
                     /locus_tag="RHV13_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HDV1242016.1"
                     /translation="MLQVTSEQWLSWLNLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            12823..13446
                     /gene="rcsA"
                     /locus_tag="RHV13_002492"
     CDS             12823..13446
                     /gene="rcsA"
                     /locus_tag="RHV13_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HDV1242017.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(13490..13678)
                     /gene="dsrB"
                     /locus_tag="RHV13_002493"
     CDS             complement(13490..13678)
                     /gene="dsrB"
                     /locus_tag="RHV13_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HDV1242018.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            13841..14068
                     /gene="yodD"
                     /locus_tag="RHV13_002494"
     CDS             13841..14068
                     /gene="yodD"
                     /locus_tag="RHV13_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HDV1242019.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            14366..15181
                     /locus_tag="RHV13_002495"
     CDS             14366..15181
                     /locus_tag="RHV13_002495"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HDV1242020.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLTRLREANVPVI
                     LCSSKTSAEMLYLQKMLGLQGLPLIAENGAVIQLAEQWQEIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(15178..16872)
                     /gene="yedQ"
                     /locus_tag="RHV13_002496"
     CDS             complement(15178..16872)
                     /gene="yedQ"
                     /locus_tag="RHV13_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HDV1242021.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRDAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(17043..17225)
                     /locus_tag="RHV13_002497"
     CDS             complement(17043..17225)
                     /locus_tag="RHV13_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HDV1242022.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(17304..18221)
                     /locus_tag="RHV13_002498"
     CDS             complement(17304..18221)
                     /locus_tag="RHV13_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HDV1242023.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            18394..19314
                     /gene="yedA"
                     /locus_tag="RHV13_002499"
     CDS             18394..19314
                     /gene="yedA"
                     /locus_tag="RHV13_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HDV1242024.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(19303..19773)
                     /gene="vsr"
                     /locus_tag="RHV13_002500"
     CDS             complement(19303..19773)
                     /gene="vsr"
                     /locus_tag="RHV13_002500"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HDV1242025.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALSERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(19754..21172)
                     /gene="dcm"
                     /locus_tag="RHV13_002501"
     CDS             complement(19754..21172)
                     /gene="dcm"
                     /locus_tag="RHV13_002501"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HDV1242026.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISGCFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(21239..21934)
                     /locus_tag="RHV13_002502"
     CDS             complement(21239..21934)
                     /locus_tag="RHV13_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HDV1242027.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSSAG"
     gene            complement(21974..22339)
                     /locus_tag="RHV13_002503"
     CDS             complement(21974..22339)
                     /locus_tag="RHV13_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDV1242028.1"
                     /translation="MEKCDFYHIIVLSLNFPGYLKMEYGSTKMEERLSRSPGGKLALW
                     AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVGAL
                     LGSIAWYTRPRPARSRRYE"
BASE COUNT         5521 a         5920 c         5708 g         5353 t
ORIGIN      
        1 attatcaaca gttagactat gcgtctccca attgataact ttttttccct gacggatacg
       61 acataaagct ggcataagag gcttaaccct gtgaatctca tgatgtttat gcatccgtat
      121 ttcttcaatc tttaggttag tcctatcttt tgccagcata ctcttatcct actttacctc
      181 ataaacgggt gttatctcag cggttgcaat tttattggca ttaagcatat aaccaactaa
      241 cgctccgctg gaattagaat ccacaggaat cttatcagtt tttagagccc atattttaaa
      301 ctggtaatga tggggtttat ctcctttggg aggacatgct ccaccaaatc ccgcatagcc
      361 aaaatcattt cggccttgga cagcaccagt cggaagtttt gtaccatcac gtcttcctgc
      421 atcagcgggc aaatatgtta ccgttgctgg aatattagca acagtccagt gccaccatcc
      481 actgcctgta ggcgcatctg gatcatacac agttacggca aagcttttgg tgccttctgg
      541 agcaccagac caggttaatg agggcgatgt attacccccc tcacacccaa atccagaaaa
      601 tacatgagac gttgtaagtt gctctcctgt ttttatttca ttactagtga cctgaaatgc
      661 tgcggcctgc gcagaaaatg ttatgaatgc caatacactt gaaacgataa gttttttcat
      721 aaaaacctct ttgttgtgac ctatcgttat tttatttgat gtgccttgat ctcattatgc
      781 aaaaaggcgc aatgatcgcg caaatgcaat cataattata aagttgcccc gaacattagc
      841 cataatatgc acaaatcaga ttcctgttat ctcaaggatg attatttttt aatgcggtgc
      901 acttcgtgac aacaagttaa tgactaacac accggcacaa atcaacatca tgcctatgat
      961 ggctggcagg tccagtcgtt gaccaaaaaa tccccaggaa agcaaactaa tcagaacgat
     1021 accgactcct gaccagatag cataagcaat ccctgtaggt atataagcca gtgtctgagc
     1081 taataaccag aatgatgcac aatagcaaat aattgtgcca acagagggcc ataaccgtgt
     1141 aaacccctct gaaaacttca ttaaggttgt accaatgacc tctgcaagta ttgcgccacc
     1201 aagataaata taagggttca tagcatattc tttcctgttc atactggaga gaattgtact
     1261 acagtttgaa cacaactcac cgttttcatc ctggtgtatg tgtatccagg gggctctggc
     1321 gtggcaagag ataaaggcag gaacaataat gatcagaagg aaccccaggc taacaaaaga
     1381 gttcactctt cctatgagca aagcgaagac aaaacatgtt atcgcgtata cagatcgtgg
     1441 atggcagaaa actttcagga tcaggtatcc attttaaacc agaaaatgag tgaatttgcc
     1501 ccatccatgc cccacgcggt gagatcggat gtaatcaaca acaggttaca aaacctacac
     1561 ctgcatgctc atcacttcct gatacgccgc caccagctta ttacgcacct gaatccccat
     1621 ttgcatagaa actgaggctt tttgcatatc ggtcatcaca tcgtttaacg ccacgccggg
     1681 ttcaccgaga gtgaattttt ctgcctgcgt gcgggcggct gtttgtgtat cgcttatgcg
     1741 atcgagagcg gcgtgcagct gcccggcaaa actaatggtc ggttgcggca gtgattcctg
     1801 cgcacgcgca ctcatcgccg tagcctgtaa ctggctgata accccttcaa tcccctgtat
     1861 cgctgacatt ttcatctcct ggataatttc tgatagcaaa gcctaccagt aagtcaataa
     1921 gacaaaggcg ctaaatagca acaaaaaaac gggtttattg gcggatagaa aaaaacgaaa
     1981 gcacaaataa tgggagcgtc aatttttcga gtttgctgac ccgggagtga gtcttgttcc
     2041 actttgccaa taacgccgtc cataatcagc cacgaggtgc gcgatgaatg cgactgcagc
     2101 ccagacaaaa tctcttgagt ggcttaatcg cctgcgtgcg aatccgaaaa ttccattgat
     2161 tgttgccggt tccgcggcag tggcggtcat tgtcgcactg atcctgtggg cgaaagcccc
     2221 cgactaccgc acattattca gcaatctttc cgatcaggat ggtggcgcaa ttgtcagcca
     2281 actgacgcaa atgaatattc cttaccgctt cagcgaagcc agcggcgcta ttgaagttcc
     2341 ggcagataaa gttcacgagc tgcgtctgcg cctggcgcaa caaggtttgc ccaaaggcgg
     2401 cgcggtcggt ttcgaactgc tcgatcagga aaagtttggt atcagccagt tcagcgaaca
     2461 ggtgaattat cagcgggcgc tggaaggcga gctttctcgt accatcgaaa ctatcggccc
     2521 ggtaaaaggg gcgcgcgtac atctggcaat gccgaaaccg tctttattcg tccgtgaaca
     2581 aaaatcccct tctgcatcgg tgacggtaaa tctgttaccc ggccgcgcac tcgatgaagg
     2641 gcaaattagc gccattgtgc atctggtttc cagcgcggtg gctggtctgc cgccggggaa
     2701 cgtcacgctg gtggatcagg gcggacatct gttaacccag tccaacacca gcgggcgcga
     2761 tcttaatgac gctcagttga aatatgccag cgatgtcgaa ggccgtattc agcggcgtat
     2821 tgaagcgatc ctgtcgccta ttgtcggcaa cggtaatatt cacgcccagg ttacggcgca
     2881 gctggacttc gccagtaaag aacaaacgga agaacagtat cgccctaatg gtgatgaatc
     2941 tcaggcggcg cttcgttcac gccagcttaa tgagagcgag caaagcggtt ccggttatcc
     3001 gggcggcgta ccgggggcgt tgtcgaatca accggcacct gcgaataacg cgccaatcag
     3061 cacgcctccg gcaaatcaaa ataaccgcca gcagcaggcg agcaccacca gcaatagtgg
     3121 gccgcgtagc acacagcgga atgaaaccag taactacgaa gtcgatcgca ccattcgtca
     3181 taccaaaatg aacgtgggcg atgtgcaacg tctgtcagtc gcggttgtgg tgaattacaa
     3241 aaccttgcca gatggcaaac cgttgcctct cagcaacgaa cagatgaagc aaattgaaga
     3301 tctgacccgc gaggcgatgg gcttttctga aaaacgcggt gattcgctca atgtcgttaa
     3361 ctcgccgttc aatagcagtg acgaaagcgg cggagaactg ccattctggc aacagcaagc
     3421 gtttatcgat cagttacttg ctgccggtcg ctggttgctg gtactgctgg tggcgtggct
     3481 gctgtggcgg aaagcggtac gtccgcagct aacacgtcgc gctgaggcga tgaaagctgt
     3541 acagcaacag gcgcaggccc gcgaggaagt ggaagatgcg gtggaagtcc gcctgagcaa
     3601 agacgaacaa ctacaacaac ggcgcgctaa ccaacggctg ggtgcagaag tcatgagcca
     3661 gcgtatccgt gaaatgtctg ataacgatcc gcgcgtggtg gcgctggtca ttcgccagtg
     3721 gataaataac gatcatgagt aacctgacag gcacagataa aagcgtcatc ctgctgatga
     3781 ccattggcga agaccgggcg gcagaggtgt tcaaacacct ctcccagcgc gaagtgcaaa
     3841 ccctgagcgc tgcaatggcg aacgtcacgc agatctccaa caagcagcta accgatgtgc
     3901 tggcagagtt tgagcaagaa gctgaacagt ttgccgcact gaatatcaac gccaacgatt
     3961 atctgcgttc ggtattggtc aaagctctgg gtgaggaacg tgccgccagc ctgctggaag
     4021 atattctcga aactcgcgat accgccagcg gtattgaaac gctcaacttt atggagccgc
     4081 agagcgccgc cgatctgatt cgcgatgagc atccgcaaat tatcgccacc attctggtgc
     4141 atctgaagcg cgcccaggcc gccgatattc tggcgttgtt cgatgaacgt ctgcgccacg
     4201 acgtgatgtt gcgtatcgcc acctttggcg gcgtgcagcc agccgcgctg gcggagctga
     4261 ccgaagtact gaatggcttg ctcgacggtc agaatctcaa gcgcagcaaa atgggcggcg
     4321 tgagaacggc agccgaaatt atcaacctga tgaaaactca gcaggaagaa gccgttatta
     4381 ccgccgtgcg tgaattcgac ggcgagctgg cgcagaaaat catcgacgag atgttcctgt
     4441 tcgagaatct ggtggatgtc gacgatcgca gcatccagcg tctgttgcag gaagtggatt
     4501 ccgaatcgct gttgatcgcg ctgaaagggg ccgagcagcc actgcgcgag aaattcctgc
     4561 gcaatatgtc gcaacgtgcc gccgatattc tgcgcgacga tctcgccaac cgtggtccgg
     4621 tgcgtctgtc gcaggtggaa aacgaacaga aagcgattct gctgattgtg cgccgccttg
     4681 ccgaaactgg cgagatggta attggcagcg gcgaggatac ctatgtctga taatctgccc
     4741 tggaaaacct ggacgccgga cgatctcgcg ccaccacagg cagagtttgt gcccatggtt
     4801 gagccggaag aaaccatcat tgaagaggcc gaacccagcc ttgagcagca actggcgcaa
     4861 ctgcaaatgc aggcccacga gcaaggttat caggcgggga ttgccgaagg tcgccagcaa
     4921 ggtcataagc agggctatca ggaaggactg gcccaggggc tggagcaagg tctggcagag
     4981 gcgaagtctc aacaagcgcc aattcatgcc cggatgcagc aactggtcag cgaatttcaa
     5041 actacccttg atgcgcttga tagtgtgatt gcttcgcgcc tgatgcagat ggcgctggag
     5101 gcggcacgtc aggtgatcgg tcagacgcca acggtggata actcggcact gatcaaacag
     5161 atccaacagt tgttgcagca agaaccgtta ttcagcggta aaccacagct gcgcgtgcac
     5221 ccggatgatt tgcaacgtgt ggatgatatg ctcggcgcta ccttaagttt gcatggctgg
     5281 cgcttgcggg gcgatcccac cctccatcct ggcggctgta aagtctccgc cgatgaaggc
     5341 gatctcgacg ccagtgtcgc cactcgctgg caagaactct gccgtctggc agcaccagga
     5401 gtggtgtaat gaccacgcgc ctgactcgct ggctaaccac gctggataac tttgaagcca
     5461 aaatggcgca gttgcctgcg gtacgtcgct acgggcgatt aacccgcgct accggactgg
     5521 tgctggaagc caccggatta caattgccgc tcggcgcaac ctgtgtcatt gagcgccaga
     5581 acggcagcga aacgcacgaa gtagaaagcg aagtcgttgg ctttaacggt caacggctgt
     5641 ttttaatgcc gctggaggaa gtggaaggtg tcctgcccgg cgcgcgtgtt tatgccaaaa
     5701 acatttcggc tgaaggactg caaagcggca agcagttgcc gctcggtccg gcgttattag
     5761 gtcgcgttct ggacggcagc ggtaaaccgc tcgatggcct gccctccccc gatacgacgg
     5821 aaaccggtgc gctgattacc ccaccattta acccgttgca acgtacaccg attgaacatg
     5881 tgctggacac cggcgtgcgc ccaatcaatg ccctgcttac cgttgggcgt gggcagcgta
     5941 tggggctgtt tgccgggtcc ggcgttggta aaagtgtgct gctggggatg atggcacgtt
     6001 acacccgcgc cgatgtcatt gtcgtgggtt tgattggtga acgtgggcgc gaagtaaaag
     6061 attttattga gaatatcctc ggtgccgaag ggcgtgcacg ctccgtggtg attgccgctc
     6121 cggcggatgt ctctccgctt ctgcgaatgc aaggtgccgc ctatgccacg cgcattgccg
     6181 aagattttcg cgatcgtggt cagcatgtgt tgctgattat ggactccctc acccgctacg
     6241 cgatggccca gcgtgaaatt gcgctggcga ttggcgaacc acccgccacc aaaggttatc
     6301 caccgtcggt gtttgccaaa ttaccggcac tggtcgagcg tgccggaaat ggcattagcg
     6361 gcggcggatc gattactgca ttttataccg tgctcactga aggcgatgac cagcaggatc
     6421 cgattgccga ctccgcgcgg gcgatcctcg acggtcacat tgtgctgtct cgccgactgg
     6481 cggaagccgg gcactatccg gctatcgata ttgaagcgtc gatcagccgc gcaatgacga
     6541 cgttgatcag cgagcaacat tacgcgcgag tgcgcacctt caaacagctg ttgtcgagtt
     6601 ttcagcgtaa ccgagatctg gttagcgtcg gcgcgtatgc caaaggcagc gatccgatgc
     6661 tcgataaagc catcgccctg tggccgcagc tggagggcta tttgcaacaa ggcatttttg
     6721 aacgcgcgga ctgggaagcg tctctccagg ggctggagcg tattttcccg acagtatcat
     6781 aacccaggag ataacggcag atggcagaac atggtgcgct ggcgaccctg aaagatctgg
     6841 cagaaaaaga ggtagaggat gccgcgcgcc tgctgggtga aatgcgtcgc gggtgtcagc
     6901 aggcggaaga acagctcaaa atgctgatcg attatcagaa tgaatatcgc aataacctca
     6961 acagcgatat gagtgccggg atgaccagca accgctggat caactatcag cagtttatcc
     7021 agacgctgga aaaagccatt actcagcatc gccagcaact taatcagtgg acgcagaaag
     7081 ttgacattgc cctgaacagc tggcgagaaa agaaacaacg tttgcaggcc tggcagacac
     7141 tgcaggaacg gcaatccacg gcggcactgc ttgcagaaaa ccgcctcgat cagaaaaaga
     7201 tggatgagtt cgcccagcgc gccgccatga ggaaacctga atgattcgct tagcgccctt
     7261 gattaccgcc gacgttgaca ccaccacatt gcctggcggc aaagccagcg atgctgcaca
     7321 agattttctc gcgttgttga gcgaagcatt agcaggcgag acaacaaccg acaaagcggc
     7381 cccccagttg ctggtggcaa cagataagcc ctcggcaaaa ggcgagccgc tggtcagcga
     7441 gattcttgcc gacgcgcaac aagcggattt actgatccct gtggatgaaa caccgcctgt
     7501 catcaacgac gaacaatcca catcaacacc gttaaccacc gctcagacga tgacgttggc
     7561 tgcggtggct ggcaacaata cggcaaaaga cgaaaaagcg gatgatctga atgaagacgt
     7621 caccgcaagc ctgagtgccc tttttgcgat gttgccgggt tttgacaata cgcccaaagt
     7681 gactgatgcg ccgtcaaccg tgttaccgac agagaaacca acgctcttca caaaactgac
     7741 ttctgcgcaa ctcacaacag cacagcctga tgacgccccc ggcacaccag ctcagccatt
     7801 aacaccgctg gtagcagaag cccagagtaa agcggaagtc atcagcacac cttcaccggt
     7861 gaccgctgcc gccagcccgc taatcactcc acaccagaca cagccactgc ccaccgtcgc
     7921 cgcacctgtt ttgagtgcac cgctgggttc tcacgaatgg caacaatcat taagccagca
     7981 tatttcgctg ttcacccgcc aggggcaaca aagtgcagag ttgcgactgc acccacagga
     8041 tttaggtgaa gtgcaaatct ccctcaaagt ggatgataac caggcgcaaa tccagatggt
     8101 ttcaccacat cagcacgtac gcgccgccct ggaagcagcg ctgccggtac tgcgtacgca
     8161 gctggccgaa agtggcattc agttagggca aagcaacatc agtggcgaaa gctttagtgg
     8221 tcagcagcag gccgcttccc agcaacagca aagccaacgc acagcaaacc atgaacctct
     8281 ggcgggggaa gacgacgata tgcttccggt tcccgtctct ttacaagggc gcgtaacagg
     8341 caacagcggc gttgatattt tcgcctaacg tcagaggtag caccgtaatc cgcgtctttt
     8401 ccccgctttg ttgcgctcaa gacgcaggat aattagccga taagcagtag cgacacagga
     8461 agaccgcaac acatgactga ttacgcgata agcaagaaaa gcaagcgatc gctttggatc
     8521 ccgattctgg tattcattac cctcgcggcc tgtgccagcg caggttacag ctactggcat
     8581 tcgcatcagg ttgccgctga cgacaaagcg cagcaacgcg tcgtgccctc accggtcttc
     8641 tatgcgctgg ataccttcac ggtcaatttg ggcgatgcgg atcgcgtgct ttatattggc
     8701 ataaccctgc gcctgaaaga tgaagctacc cgctcgcggc tgagtgagta tttgccggaa
     8761 gtccgtagcc gtttgctgtt actgttttcg cgtcaggatg ctgccgtact ggcgacagaa
     8821 gaaggcaaga aaaacctgat tgccgagatt aaaaccacac tttccacccc gcttgttgcc
     8881 gggcaaccga aacaggatgt caccgacgtg ctgtataccg cttttattct gcgataacga
     8941 catgggcgat agtattcttt ctcaagctga aattgatgcg ctgttgaatg gcgacagcga
     9001 agtcaaagac gaaccgacag ccagtgttag cggcgaaagt gacattcgtc cgtacgatcc
     9061 gaatacccaa cgacgggttg tgcgcgaacg tttgcaggcg ctggaaatca ttaatgagcg
     9121 ctttgcccgc cattttcgta tggggctgtt caacctgctg cgtcgtagcc cggatataac
     9181 cgtcggggcc atccgcattc agccgtacca tgaatttgcc cgcaacctgc cggtgccgac
     9241 caacctgaac cttatccatc tgaaaccgct gcgcggcaca gggctggtgg tgttctcgcc
     9301 gagtctggtg tttatcgccg tggataacct gtttggcggc gatggacgct tcccgaccaa
     9361 agtggaaggt cgcgagttta cccataccga acagcgcgtc atcaaccgca tgttaaaact
     9421 ggcgctagaa ggctatagcg acgcatggaa ggcgattaat ccgctggaag ttgagtacgt
     9481 gcgttcggaa atgcaggtga aatttaccaa tatcaccacc tcgccgaacg acattgtggt
     9541 taacacgccg ttccatgtgg agattggcaa tctgaccggc gaatttaata tctgcctgcc
     9601 attcagcatg atcgagccgc tacgggaatt gttggttaac ccaccgctgg aaaactcgcg
     9661 taatgaagat cagaactggc gcgataacct ggtgcgccag gtgcagcatt cacagctgga
     9721 gttggtcgcc aactttgccg atatctcgct acgcctgtcg cagattttaa aactgaaacc
     9781 cggtgacgtc ctgccgatag aaaaacccga ccgcatcatc gcccatgttg acggcgtccc
     9841 ggtgctgacc agtcagtacg gcactctcaa cggtcagtat gcgttacgga tagaacattt
     9901 gattaacccg attttaaatt ctctgaacga ggaacagccc aaatgagtga catgaataat
     9961 ccggccgatg acaacaacgg cgcaatggac gatctgtggg ctgaagcgtt gagcgaacaa
    10021 aaatcaacca gtagcaaaag cgctgccgac gcggtgttcc agcaatttgg cggtggtgat
    10081 gtcagcggaa cgttgcagga tatcgacctg attatggata ttccggtcaa gctgaccgtc
    10141 gaactgggcc gtacgcggat gaccatcaaa gaactgttgc gtctgacgca agggtccgtc
    10201 gtggcgctgg acggtctggc gggcgaacca ctggatattc tgatcaacgg ttatttaatc
    10261 gcccagggcg aagtggtggt cgttgccgat aaatatggcg tgcggatcac cgatatcatt
    10321 actccgtctg agcgaatgcg ccgcctgagc cgttagtgat gaataaccac gctactgtgc
    10381 aatcttccgc gccggtttct actgcgccac tgctgcaggt gagcggcgca ctcatcgcca
    10441 ttattgccct gatcctcgct gctgcctggc tgataaaacg gttgggattt gctcctaaac
    10501 gcactggcgt taacggtctg aaaattagcg ccagtgcttc actgggcgcg cgtgaacggg
    10561 ttgtggtggt cgatgtggaa gatgcacggc tggtgctcgg cgttaccgca gggcaaatca
    10621 atctgctgca taaacttccc ccttctgcac caacggaaga gataccgcag accgattttc
    10681 agtcggtcat gaaaaatttg cttaagcgta gcgggagatc ctgatgcgtc gtttgttgtc
    10741 tgtcgcacct gtccttctct ggctggttac gcccctcgcc ttcgcgcaac tgccgggtat
    10801 caccagccag ccgctgcctg gcggtggaca aagctggtcg ctcccggtgc agacgctggt
    10861 gttcatcacc tcgttgacgt ttattccggc aattttactg atgatgacca gtttcacccg
    10921 catcatcatt gtttttggtt tattgcgtaa cgcgctggga acgccctccg cgccacctaa
    10981 ccaggtattg ctggggctgg cactattttt gacctttttt attatgtcac cggtgatcga
    11041 caaaatttat gtcgatgcgt accagccatt tagcgaagag aaaatatcaa tgcaggaggc
    11101 gctggaaaaa ggggcgcagc cgctgcgtga gtttatgctg cgtcagaccc gtgaggcaga
    11161 tttagggttg tttgccagac tggcgaatac cggcccgttg cagggacctg aagccgtgcc
    11221 gatgcgcatt ttgctcccgg cctacgtgac cagcgagttg aaaaccgcat ttcagatagg
    11281 ctttacgatt ttcatccctt ttttgattat cgacctggtg atagccagcg tgttgatggc
    11341 attggggatg atgatggttc ccccagccac cattgctctg ccctttaaac tgatgctgtt
    11401 tgtactggtg gatggctggc aattgctggt cggttcgctg gcgcagagct tttacagcta
    11461 gagaggcaaa atgacacctg aatcggtcat gatgatgggg actgaagcga tgaaagtcgc
    11521 gctggcactg gctgccccgc tattgttggt agcgttggtc acgggcctta tcatcagtat
    11581 tttgcaggcc gccacgcaga ttaacgaaat gacgctgtcg tttattccga aaatcatcgc
    11641 cgtatttatc gccattatta ttgccggacc gtggatgctc aatctgttgc tggattacgt
    11701 ccgcaccttg ttcactaacc tgccgtatat catcgggtag ccgtactatg ttgcaggtga
    11761 caagcgaaca atggctatcc tggttaaacc tgtacttctg gccgttactg cgcgtgctgg
    11821 cgctgatctc caccgcgccg attctgagcg aacgcagcgt accgaaacgg gtaaaactgg
    11881 gtctggcaat gatgatcacg ttcgccattg ccccatcatt acctgccaac gatgttcctg
    11941 ttttttcgtt ctttgctctg tggctggccg tgcagcagat cctgatcggc attgcgcttg
    12001 gttttaccat gcaatttgcc tttgccgctg tacgaaccgc tggcgaaatt atcggtctgc
    12061 aaatggggct gtcatttgcg acgtttgtcg atccggccag ccatctcaat atgcccgttt
    12121 tagcgcgtat catggatatg ctggcgttac tgctgttcct gacatttaac ggtcatttat
    12181 ggttgatttc actgctggtc gatacctttc acaccctgcc aattggtggc gaaccgttga
    12241 acagcaatgc gtttctggca ctcaccaaag cagggagttt gattttcctt aacgggctga
    12301 tgctggcatt accgctcatt actctgctgc tgaccctgaa tctggcatta ggcttactta
    12361 atcgtatggc cccgcaatta tccatttttg ttattggatt tccattaact ctgactgtcg
    12421 gtatctcttt aatggcggca ttaatgccgt taattgcacc tttttgcgaa catttattca
    12481 gtgagatgtt taatttgctg gctgatatta ttagtgaatt gccattaata taattccgta
    12541 atgtttatca tgttatccta aggattatcc gaaaaataat acctacgaac atcttccagg
    12601 atactcctgc ggcgaaatat ttgttttaag ctcactcaca tatcgcaaca tttactttac
    12661 tttaagacaa ttccaggcaa attatacaac actttacggg atagtaagtc cgcctgaaaa
    12721 atcgcgagag tggcgcatta ggtgacccat gttgttccgt ttagtcatga tgaaatattc
    12781 aggtaagggg aattatcgtt acgcattgag tgagggtatg ccatgtcaac gattattatg
    12841 gatttatgta gttacacccg actaggttta accgggtatc tgttgagtag aggggttaaa
    12901 aaaagagaaa tcaacgacat tgaaaccgtt gatgaccttg ccatagcttg tgattcacag
    12961 cgcccttcag tggtgtttat taatgaggac tgtttcatcc atgatgcttc taacagtcag
    13021 cgcatcaagc acatcattaa tcaacatccc aatacgttat ttatcgtttt tatggcaatt
    13081 gccaatgttc attttgatga atatctattg gtcagaaaaa atttattgat cagttctaaa
    13141 tcgattaaac cggaatctct cgacgatatc cttggcgata ttctgaaaaa agagacaacg
    13201 ataacctctt ttttaaatat gccgacgtta tcattgagcc gaaccgaatc gagtatgttg
    13261 cgaatgtgga tggcaggtca gggaaccatt caaatctctg accaaatgaa tatcaaagcc
    13321 aagaccgttt catctcataa aggtaatatt aaacgtaaga tcaaaacgca taataaacag
    13381 gttatctacc atgtcgtccg actgacggat aatgtgacta atggtatttt tgtcaacatg
    13441 cgctaacaca ttctgactgg tggtttccca ccagtcaggc tgaataagat tactctgctt
    13501 tctccacaaa gataccgtcc tgatgccctg cttcattaaa gaaccagatg ccgagcgggt
    13561 agtcttccag cgaaaccagg tacattgtgc cttcactaaa ctcctcaact gccagtacca
    13621 cgccaggacg acgcggaccg ccatccgttt tgactgttac ccgatcattc accttcatcg
    13681 ttttcctcct gtggctttgt gccagtgtag aacaatttcg tttttctggc agcgccaggc
    13741 gcgcgcgagt gctgattttc tcgacggtct atacttaaga gatgccaggc ggacttaacg
    13801 actggcggca acaacagagt aacggttgcg aggaaagatg atgaaaaccg caaaagagta
    13861 cagcgatacc gcaaaacgtg aggtcagcgt cgatgtcgat gccctgctgg cggcgatcaa
    13921 tgaaattagc gaaagcgaag ttcatcgcag ccagaacgat tctgaacacg ttagcgtcga
    13981 tggacgtgaa tatcatacat ggcgtgaatt ggcggatgcc ttcgaactgg atattcatga
    14041 cttcagcgtc tctgaagtga atcgttgaat gcacaataaa aaaatcccga ccctgagggg
    14101 gtcgggatga aacttgctta agcaagaagc acttaaaaaa ttcgttacac caggaaatct
    14161 gatgtgttca tcaccttatc cgcaattttt ttcgctgaca agaaaatatt cgccatatga
    14221 atgattaacg ctacttatga atagaaatgt gacgcacgtc accccttggt ggctctggcg
    14281 tcagcgggat cgctcccgaa aaaatcatct ttgataattt gtgctgtttt gagaatcatc
    14341 ctggtaaggg gagtactgcg cgacaatgtt ttcaattcaa caaccactac tggtttttag
    14401 cgatcttgat ggcaccctgc tggacagtca tagttatgac tggcaaccgg cagccccctg
    14461 gctcacccgt ttacgcgaag caaatgttcc agtcattctc tgtagcagta aaacatcagc
    14521 ggaaatgctg tacttgcaaa aaatgttggg gctacaaggt ttaccgctga ttgcagagaa
    14581 tggcgcagtg atccagcttg ctgaacaatg gcaggagata gacggttttc cacgcatcat
    14641 ctcaggtatt agccatggcg aaatcagcca ggttttaaat acgctacgcg agaaagaaca
    14701 ttttaaattc acgacttttg atgatgtcga cgatgcaacc atcgccgaat ggacgggatt
    14761 aagccgtagc caggcggcgc tgacgcagct tcatgaggcg tcggtaacgc taatctggcg
    14821 cgacagtgac gagcgtatgg cacaatttac cgctcgtctg aacgaactgg gcttacagtt
    14881 tatgcagggt gcgcgcttct ggcacgtact ggatgcctct gccggaaaag atcaggctgc
    14941 caactggatt atcgcgacct atcaacaatt gtcaggcaaa cgcccaacca cacttggcct
    15001 gggcgatggg ccaaacgatg cgcccttact ggaggtaatg gattacgcgg tgattgtgaa
    15061 agggctaaac cgtgaagggg tgcatctgca tgatgaggat ccggcccgcg tctggcgaac
    15121 gcagcgtgaa ggaccggaag gatggcgtga agggctggac cattttttct ccgcccgtta
    15181 agcgttatcg ctcgcgaata cccgattacg cccagcctgt ttagcgagat aaagccgacg
    15241 gtcggccagt gactggagtt gttcaaaatc ataatcaccg gtttcctcgc tgctacttac
    15301 ccccaacgag gcactgatgc gtatcgtcgt actcttagca atcaacatct ctttttcatt
    15361 taacttcagg cgaatacgtt ctgcgacttc cgcagcctcc gtcagactcg cgcctggcag
    15421 aatcacacaa aactcctcac caccgacccg cccggcaacg tcctgcgcac gcaaggaact
    15481 gctaattaat ccggcagcat gagaaagaac acggtcgccc gcctgatgac caaagcggtc
    15541 attaatcgct ttaaaatggt caaggtcgac ctgaatgaca gaaaaaggat gttggtgcgt
    15601 ctgacacaat ttagcgagcg gacgggcttt ttcgaacagt gcgccacgat tatataaacg
    15661 ggttaaggtg tcgtgccacg cctgccactg caacgagctt tgcagaacat acatgttgct
    15721 gaccatccgg cgaatcacat accaggagat gagtaacatg gtggtaaaga gcgcccacag
    15781 cagggttaat gcaatgctga tactgccgaa atcgccgcgc acgccttcgc ttagcgtatg
    15841 gacgcgcacc agcacaccgt cgaaatggtc cagacgttcc cagctaacat agcgactgtc
    15901 catacgaatg ccgccccgcg tgtcatgttc catcgcctgc gccagcaagg ccagttcacg
    15961 aggatcaaaa atattccctg ttggatggtc aggattggaa gaggtcaaaa atctcagctt
    16021 actgtcatag agctgatact caccatcgag gtttttatcg atggcgtttc ttaaaaattg
    16081 ctgcatagta cgtacgggaa tactcatccc cagcacgcca taccagtagt tattactgtc
    16141 taccggaaca ctgacggtaa cctgcggttc agtattgctg gcgtgttccg gttgcgaagt
    16201 gaaccagcgt accgcgcggt gacgattttc tcgttgtgaa tggccgataa accagggttg
    16261 ggtgacatag ccgtaataac gcgttggaac attgcgcgta aacaaggtcg gctgcgtcga
    16321 aacgtaaaat ccggcacgcg agacatacat cgcctgttca accatcgacg aggagttgtg
    16381 cgccagtcgc agcaagtaac caacttccag tgcagcggta atttcattgt cgaggctttc
    16441 attttcgcga gacaggagat ttccctcgct gactaatgca tccgatacgc cattgactgg
    16501 cagggtgcgt cgtcggttga gttcgatttg ccaggcgtgc tcgtcgcgat gctgttcgaa
    16561 ctcggttaca gcatcacgca gagaggtgaa atccagtggc gctacgagag cttcgcgcat
    16621 gccattacgc aaaaagatca gtttgtcgac gttatactgc aaatgcttat cgagcgcgtt
    16681 ggctacgttt tccagatgat tacgctggct ggagatatag gcatcttcca gcaccactac
    16741 ttcacgccag gtgagcaagg tggaaaaaag caataccacg ataaagcaga gattaacgac
    16801 atgaccaggc cccaggcggc gtgcgagttt tttcaaccag ctctggtttt ccatttttgt
    16861 ctcgtgctgc accctgacga ctcccaacct gcttttttat gattctggca taacctggcg
    16921 atagcgtacc gcaaaacagc gataatttta taatggtggc atgataagac gcgactatca
    16981 ttcattgcag gcattggcac accaggccgg aagtgatccg gcctggtgtt aatgataccc
    17041 tttcaaactt cttccgcaga acgactcccc tcccccggaa caagctcggt ttcatgaaaa
    17101 gcttcccgct tgaccccgta accgtcatac caacgacact caaccatacc gctggagtat
    17161 ccggtgacaa tcatccgcgg gccgccctct ttaaccgtaa cttcctcact aaccataaag
    17221 ctcatactcg cctccttttt tcgagtgaaa cttgttcacc ttagttgaag atggcgaatt
    17281 ttgcataata gcctgtgcga gtattaatgt gcctgaccgc gcattttcgc tacggctttc
    17341 actcccagca ccacgatgcc gccgatgata aatccaagaa tcagatttaa aacagtcggt
    17401 aatatcatcg ccaccactgc actttgctgc ccggcgaaat gttcaatggc gtgatgcagc
    17461 ggcgcaatac catgtaccac aatcccgccg ccgacgagga acatcgccag cgtgccgaca
    17521 atcgataacg ctttcatcag ccagggcgcg ataatcaata atcctttacc taatgcctgc
    17581 atcagcgcgc tggatttttc cgccagccaa taccccaggt catcaatctt cacgataacc
    17641 cctaccagac cgtagacgcc cacggtcact accagcgcga tgcctgaaag caccagcacc
    17701 tgattaagca acggcgcttc ggccacaatc cccagcgtga tggcgacgat ttcggcagac
    17761 aatataaaat cggtacgaat cgcccctttt attttgtcct tttcaaactt cagcggatcc
    17821 tgcgccgcca gcttctccag acgctgctgg ctctgcgccg gatcttcttt atgtttacgc
    17881 gcctccagca tatgcagcac tttctctact ccttcaaagc agagaaacgc gccaccaatc
    17941 atcaacagag gcgtaatcgc ccacgggata aacgcactga tgatcagcgc cagcggcacc
    18001 agaatcactt tattaatcag cgatcctttc gccacgcccc agaccacggg aagttcccgg
    18061 ttggcccgca cgcctgaaac ttgttgcgca ttgagcgata agtcatcccc taatacaccg
    18121 gcggttttct tcgccgccag tttgcccatc acggagatat cgtccagtag tgtggcgata
    18181 tcatcgagca acgtcagtaa actactcccg gccaaaagca ttccctctca tttttattgt
    18241 tgaagggtgt aagtatgaag caaaaacggc agctctgata cttgcttcac aggtcaacat
    18301 ttttttaaca atcaagtgca attatatctc tcgccagcag aatgattttc acgtttacta
    18361 tgaaccacct ttttatttcc accgtgaggg attatgcgtt tccgccagtt gttaccgctt
    18421 tttggcgcgc tgtttgcgtt gtatatcatt tggggctcaa cctattttgt cattcggatt
    18481 ggcgtggaaa gctggcctcc gttaatgatg gcgggcgttc gattcctggc agccggtatt
    18541 ttattgctgg catttttgct actgcgcgga cacaaactcc ccccgctacg tccgctgctc
    18601 aatgccgcgc tgattggcct gttattgctg gctgtcggta atggcatggt gacggttgcc
    18661 gaacatcaaa atgttccttc cggcatcgcc gccgtagtgg ttgcaaccgt gcccctcttt
    18721 accctgtgct tcagccgcct gtttggcatt aaaacgcgca aactggaatg ggtgggtatt
    18781 gccattgggc ttgccggaat catcatgctc aatagcggtg gaaatttaag cggcaatccg
    18841 tggggcgcga ttctgatttt aatcggctcg attagctggg cgtttggctc agtttatggc
    18901 tcgcgcatta ccttacctgt agggatgatg gcgggtgcga ttgagatgct ggcggcaggc
    18961 gtggtgttaa tgatcgcgtc gatgattgcg ggtgaaaaac tgacggcgct cccttccctt
    19021 tcaggcttcc ttgcggtcgg ctatctggcg ctgtttggtt cgattatcgc catcaacgct
    19081 tatatgtatt taatccgtaa tgtcagtccg gctctcgcca ccagctacgc ttacgttaac
    19141 ccggtggtcg cggtcttgct gggtacggga ctgggtggag aaacactgtc gaagattgaa
    19201 tggctggcgc tcggcgtaat tgtcttcgcg gtggtactgg taacgttggg aaaatatctc
    19261 ttcccggcaa aacccgtagt tgcgccagtt attcaggacg catcaagcga gtaaatgaat
    19321 cccctgcgtg tcgatctgcg cgctggcacc ttcgccgcag atccactctt ccagacgctc
    19381 gctaagcgct tcatccgtca gcttctcgcg cccacgtaac gcgcactccc agacaatcaa
    19441 tacgcgccag ccgagttcct gcaagcgact gatatcgcgg cgatcgcgct caacattttt
    19501 acctatcttc tccagccaga actcggttcg agtcgcaggc actttaaaca gatagcagtg
    19561 atgatgatgc cagaagcagc cgtgggtaaa tatcacgcag cgatattcat caacgacaaa
    19621 atccgggcgt ccgggcagac tggcgtcctg aacgcgaaac gccaggccct gcccggttaa
    19681 caaactggcg aggcgcttct caatcgccgt atcacgcgtg gcaatcgcgc gcatattttt
    19741 gctgcgcgtg gccttatcgt gaacgtcggc catgttgtgc ctcttgctga cgcaacgcca
    19801 ccgcctgttt gatttttggc tcaagcagtt ttgccaccgc ggcaaagact ggcacgacca
    19861 ccgagttacc gaactggcga taggcctgag tgtccgaaac cggaatgcgg aatttcgctt
    19921 ctcccggcgc ttcaaaaccc attaagcgcg cgcattcccg aggcgttaac cgacgtggac
    19981 gatgttgctg attcagcgga tcgtcaaagt ctttttcacc cgtggccata tcccatccgc
    20041 gatcgattaa aatttccgcg ccatctttgt aataacgcgc agacagcgta cgcgtgacgc
    20101 tttgcggatt gttcggataa accattccat aaccgaagcc gttaccgcgc gcctgatgtt
    20161 ttttcgcata acgatagagg tacttccaca gcaccggcgt caggatatat ttcgcctcga
    20221 ccatcgggtc cagcagctgc gccagcgtca ctcgctgcgc agggaaacat ccgctgatat
    20281 cacgcagggt aaaatcggct ttcagattca gatcgcgacg aaaacccacc agcacgatgc
    20341 gttcacggtg ctgcggcaga aaatgtttac catcgatgat tttcggatcg tccggcccgt
    20401 tatcttctgc atcagccacg tcatagccca gttcgtccag cgtctgcatg atgatgcgga
    20461 acgttttacc ctggtcgtgg cttttcaggt ttttgacgtt ttcgagcaca aacatcgccg
    20521 gacgacgcgc gtcgataatg cgcaccacat caaagaacag cgtgccctga gtatcgcagg
    20581 caaaaccgtg cgcccgcccg agcgagttct ttttcgatac gccagccagc gaaaatggct
    20641 gacaagggaa accggccagt aaaacatcgt gttcaggaat gtgttgacga atatgttccg
    20701 ccgccgcctc atcactcacg ccttctttat ggctgagggt gatgtcgcgg atatcttcat
    20761 taaaatgatg cgtcgccggg tcgcaataat gattggcttt ataagtgcgt accgcatgtt
    20821 tgttccattc gctggtaaac acgcattgtc cgccaatcga ttcaaaaccg cgacggatgc
    20881 cgccaattcc ggcaaataga tcgataaagc gaaacgcata atgcggatga tgtgccggtg
    20941 gtttgggtaa caacgtttgc agatgggcga actctttctc acttaaacgg tgccatgccg
    21001 agtcattcgc cagcgcacgt tttaaaattg ccgcgctcca gtgattctca cccacaccat
    21061 taagctgcgc caccagcgtt ttaacatcat aaatttgcag cagtttctcc agcattgcct
    21121 gtgcggcatt cccggtgctg tatgaatcgg ttactgatat attttcctgc atagatttca
    21181 ccggccatct gagatggccg acaggttaac ataattacag ccatttaggg ccacgcgctc
    21241 agccagcgga tgaaaacgca tctattacct tgtgatcgac accttcattc tcccccgcca
    21301 gttcggcact gagcttcgcc ataaactcga ctaaaaaatg cgcgttgtgc tgcgccagct
    21361 gcttgcctct tgcggtttgc atggtttgcg gtagcttcag caacttagtc tggaaatgat
    21421 ccagcgcgta gcgtttatca tcaagcgggc gatgctgtgc gaacggatct tcaccatcaa
    21481 acagcgccac gcccaatgcc cctgaaacgg caaacacgcg cgccaggcca atcgccccca
    21541 gggcttccag ccgatccgca tcctggacga ttttagcttc cgttgttaag ggggcgattt
    21601 gcgcgctgaa actgtgagcg gcaatggcat gacaaacggc ctcgattttc tccgccggaa
    21661 attgctcgaa ctcttcacgg agcaggcgac gcgtctcttc tgccgccagg attgaagaac
    21721 gctgcctttg cggatgattt ttcgccaggc tcacaatatc gtgaaaataa caggcggtca
    21781 aaatcaccag catatcgaca tcgtcatctg ccgccagctt ctgtgccgta gcccagacgc
    21841 ggcgaaaatg acacacgtca tgggccgcgt cctggtgctg atgatggttt ttcaaccagt
    21901 tttcgaactg tgcctgccag tgttgtaagt ccatacgcgc tccgttgcca aagagtcgcc
    21961 accttagcag tttttattca tagcgtctgc tacgtgcggg acgaggtcgg gtgtaccagg
    22021 caatgctgcc taacaatgcc ccgaccagag ccataaataa aaatccgacc agtgccccta
    22081 accactttcc cgcggtagag cctaaatcgc tttcaaatcc cgacacgggg gcatcgggga
    22141 tccggctcat cacccagatg taacgcgcca ttgcccagac gaaatagcca caccatgtat
    22201 agaaagccca caaagccagt ttgccgccag ggctgcgaga gagtctctct tccatctttg
    22261 tcgaaccgta ttccattttg agataacccg gaaaattcag acttagtact attatgtgat
    22321 aaaagtcaca tttttccaca ttggatgagc cagtcaagac tcaatcattt tttgaacaaa
    22381 tagataaatt ttgcgcaacg gcagtgaata caggaaaaat cgttttgagc gattttattt
    22441 atctttgata tcggctttat taattcattt aatcaatata ttagcactga ttacaattat
    22501 ac
//
LOCUS       DAPGOF010000076        22044 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.076, whole genome shotgun sequence.
ACCESSION   DAPGOF010000076 DAPGOF010000000
VERSION     DAPGOF010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22044
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.076"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            170..505
                     /locus_tag="RHV13_002504"
     CDS             170..505
                     /locus_tag="RHV13_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HDV1242029.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            870..1313
                     /gene="yjdN"
                     /locus_tag="RHV13_002505"
     CDS             870..1313
                     /gene="yjdN"
                     /locus_tag="RHV13_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HDV1242030.1"
                     /translation="MPLSPYISFAGNCADAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAMPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            1446..2234
                     /gene="phnC"
                     /locus_tag="RHV13_002506"
     CDS             1446..2234
                     /gene="phnC"
                     /locus_tag="RHV13_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HDV1242031.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRAHTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYNGSSQQFDNERFDHLYRSINRVEENAKAA"
     gene            2259..3275
                     /gene="phnD"
                     /locus_tag="RHV13_002507"
     CDS             2259..3275
                     /gene="phnD"
                     /locus_tag="RHV13_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV1242032.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLSFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPSDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLAFFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3381..4160
                     /gene="phnE"
                     /locus_tag="RHV13_002508"
     CDS             3381..4160
                     /gene="phnE"
                     /locus_tag="RHV13_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HDV1242033.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            4181..4906
                     /gene="phnF"
                     /locus_tag="RHV13_002509"
     CDS             4181..4906
                     /gene="phnF"
                     /locus_tag="RHV13_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HDV1242034.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGIAEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4907..5359
                     /gene="phnG"
                     /locus_tag="RHV13_002510"
     CDS             4907..5359
                     /gene="phnG"
                     /locus_tag="RHV13_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HDV1242035.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQTRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5356..5940
                     /gene="phnH"
                     /locus_tag="RHV13_002511"
     CDS             5356..5940
                     /gene="phnH"
                     /locus_tag="RHV13_002511"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HDV1242036.1"
                     /translation="MTLETAFILPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLSAPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAVPRTTHVEVC"
     gene            5940..7004
                     /gene="phnI"
                     /locus_tag="RHV13_002512"
     CDS             5940..7004
                     /gene="phnI"
                     /locus_tag="RHV13_002512"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HDV1242037.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPYFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            6997..7842
                     /gene="phnJ"
                     /locus_tag="RHV13_002513"
     CDS             6997..7842
                     /gene="phnJ"
                     /locus_tag="RHV13_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HDV1242038.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     VIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            7839..8597
                     /gene="phnK"
                     /locus_tag="RHV13_002514"
     CDS             7839..8597
                     /gene="phnK"
                     /locus_tag="RHV13_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HDV1242039.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYGMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8708..9388
                     /gene="phnL"
                     /locus_tag="RHV13_002515"
     CDS             8708..9388
                     /gene="phnL"
                     /locus_tag="RHV13_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HDV1242040.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            9385..10521
                     /gene="phnM"
                     /locus_tag="RHV13_002516"
     CDS             9385..10521
                     /gene="phnM"
                     /locus_tag="RHV13_002516"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HDV1242041.1"
                     /translation="MIINNVKLVLENEVVHGSLEMQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARKIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDQSNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            10521..11078
                     /gene="phnN"
                     /locus_tag="RHV13_002517"
     CDS             10521..11078
                     /gene="phnN"
                     /locus_tag="RHV13_002517"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HDV1242042.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRQREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGKEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPICLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            11065..11499
                     /gene="phnO"
                     /locus_tag="RHV13_002518"
     CDS             11065..11499
                     /gene="phnO"
                     /locus_tag="RHV13_002518"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HDV1242043.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLHD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            11501..12259
                     /gene="phnP"
                     /locus_tag="RHV13_002519"
     CDS             11501..12259
                     /gene="phnP"
                     /locus_tag="RHV13_002519"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HDV1242044.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACARARRSPHYRRQPCSGVA
                     KFNEAITLIDAGRHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRNHCDLNTVLALNEVIRSPRV
                     ILTHISHQFDAWLMENALPPGFEAGFDGMEIGVA"
     gene            complement(12302..12631)
                     /locus_tag="RHV13_002520"
     CDS             complement(12302..12631)
                     /locus_tag="RHV13_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242045.1"
                     /translation="MKRHSTLFFFTLLTLTTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12700..14961)
                     /locus_tag="RHV13_002521"
     CDS             complement(12700..14961)
                     /locus_tag="RHV13_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDV1242046.1"
                     /translation="MAHPGRHFFASARGRLLLLNLLVVAVTLMVSGVAVMGFRHASQM
                     QELVQQQTVDDMTGSLNLARDTANVATAAVRLSQVVGALEYKGEAERLQETQRALKSS
                     LAQLANAPLAQQEAGLVTRIITRSNELQTSVGGMLERGQRRHLERNALLSSLYQNLSY
                     LRHLQKVTHAQDDILLNEMNRLIVAAIATPAPQAIIHQLVGVMSALPTHSDTPLVNTL
                     LNDFNRELRKLAPLSAALEQSDLAISWYMFHIKALVAILNGDINRYAEQVATLSGQRV
                     AQSYQDLRSGERVIMIFALLAVVITAFAGWYIYRNLGTNLTAISRAMSRLAHGEADVT
                     FPALQRRDELGELARAFNVFARNTESLERTSRLLKEKTTQMEIAQTERQGLEEALLHS
                     QKLKAVGQLTGGLAHDFNNLLAVIIGSLELVKPDSPDAPRIQRALQAAERGALLTQRL
                     LAFSRKQSLHPQAVELKTLLEELSELMRHSLPATLALEIEAQSPAWPAWIDISQLENA
                     IINLVMNARDAMEGQTGIIKIRTWNQRVTRSSGQRQDMVALEVIDHGSGMSQAVKSQV
                     FEPFFTTKQTGSGSGLGLSMVYGFVRQSGGRVEIESAPGQGTTVRLQLPRALTAAKNL
                     SPAAVEQASVSGDKLVLVLEDEAAVRQTICEQLHLLGYLTLEASSGEQALDLLAASAE
                     IDIFISDLMLPGGMSGAEVVNAARKLYPHLTLLLISGQDLRPSHNPALPDVALLRKPF
                     TRAQLAQALGQEN"
     gene            15075..16595
                     /locus_tag="RHV13_002522"
     CDS             15075..16595
                     /locus_tag="RHV13_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDV1242047.1"
                     /translation="MNSTPVLEMRNIAKAFGKFYALKGVDLTVYPGEIHALMGENGAG
                     KSTLMKVLAGAYTATSGEILIDGKPFHIRTPKDALSAGITLIYQEMQLAPNLSVAENI
                     FLGSELSHGGLVQRKEMLVQAQKVIDRLGAQFNASDKVMTLTIAEQQQVEIARALHRN
                     SRILVMDEPTAALSSRETHRLFELIMRLRDEGMAIIYISHRMAEVYELSDRVSVLRDG
                     QYVGSLTRDNLNAGELVRMMVGRPLSDLFNKERDIPLGKARLNVHHLTDGGKVQPSSL
                     LVRSGEIVGLAGLVGAGRSELAQLIFGVRKATGGMIEVDGEPVVIHSPREAIDLGIGF
                     LTENRKEQGLFLEMAAAENITMATLERDARWGMLNRKKAQTISDDAIKLLNIRVPHAQ
                     VRAGGLSGGNQQKLLISRWVAIGPRILLLDEPTRGVDVGAKSEIYRIMNEMARKGVAI
                     LMISSELPEIVGMSDRVYVMREGSIAGELNGKNITQENIMTLATGVNDAHSQAVTS"
     gene            16592..17566
                     /locus_tag="RHV13_002523"
     CDS             16592..17566
                     /locus_tag="RHV13_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HDV1242048.1"
                     /translation="MTTSAKTSAKKMLMSDLMQTVGILPILILIVAVFGFIAPNFFTE
                     SNLLNITRQASINIVLAAGMTFIILTGGIDLSVGSILGTTAVAAMVVSLSPEFAVLSI
                     PAALMLGLLLGLFNGALVAFAGLPPFIVTLGTYTALRGAAYLLADGTTVINSNINFEW
                     IGNNYLGPVPWLVVIALAVIVVCWFILRRTTLGVHIYAVGGNMQAARLTGIKVWLVLL
                     FVYGMSGLLSGLGGIMSASRLYSANGNLGMGYELDAIAAVILGGTSFVGGIGTITGTL
                     VGALIIATLNNGMTLMGVSYFWQLVIKGAVIIIAVLIDKYRTRHHQSA"
     gene            17585..18526
                     /locus_tag="RHV13_002524"
     CDS             17585..18526
                     /locus_tag="RHV13_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDV1242049.1"
                     /translation="MRLKPLVTALCAGALLAAAPFAQAKELKSIGVTVGDLANPFFVQ
                     ITKGAELEARKLAGDNVKVTLVSSGYDLGQQVSQIDNFIAANVDMIILNAADSKGIGP
                     AVKRAKDAGIVVVAVDVAAEGADATITSDNTQAGEMACKYITDRLKGKGNVVIINGPP
                     VSAVQNRVEGCQTEFKRHPDIKVLSDNQNAKGSREGGLEVMTGLLAANPKIDGVFAIN
                     DPTAIGADLAAKQAQRNEFFIVGVDGSPDAEEALKRENSLFVATPAQDPQVMAAKAVE
                     IGYDILQGKPAPIEPVLIPVTMIDKNNVGNYKGWTVK"
     gene            18581..19264
                     /locus_tag="RHV13_002525"
     CDS             18581..19264
                     /locus_tag="RHV13_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HDV1242050.1"
                     /translation="MKRSAINDILGHTRQFFSQHDVHLPPFASFSPAQWQQLDTAAWE
                     EVFDLKLGWDVTAFGRNNFAAHGLTLFTLRNGSAKGMPYVKCYAEKIMHVRDAQVTPM
                     HFHWRKREDIINRGGGNLIVELWNADSNEQTADSDITVVIDGCRQKHTAGTQLRLSPG
                     ESICLPPGLYHSFWAETGFGDVLVGEVSSVNDDDHDNHFLQPLDRYNLIDEDEPAQLV
                     LCNEYRQFR"
     gene            19279..20139
                     /locus_tag="RHV13_002526"
     CDS             19279..20139
                     /locus_tag="RHV13_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose 1,6-bisphosphate aldolase"
                     /protein_id="HDV1242051.1"
                     /translation="MPLISLAEGLAHAREHHYSLGAFNVLDSHFLRALFAAARQERSP
                     FIINIAEVHFKYISLDSLVETVKFEAARHAIPVVLNLDHGLHFESVVQALRLGFSSVM
                     FDGSTLGYEENVRQTREVVKMCHAVGVSVEAELGAVGGDEGGALYGHADESLFTDPQL
                     ARDFVDRTGIDALAVAIGNAHGKYRGEPKLDFPRLDAIRQQAAIPLVLHGGSGISDAD
                     FRRAIELGIHKINFYTGMSQAALAAVEARMAHRQPMYDEFAELLLAVEAAISDTVAQQ
                     MRIFGSAGQA"
     gene            20136..21137
                     /locus_tag="RHV13_002527"
     CDS             20136..21137
                     /locus_tag="RHV13_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HDV1242052.1"
                     /translation="MMERKGIIAAGNMLVDHVHQIIQWPERGWLAEITHSERSTGGAP
                     LNVLLTLAKMRTGLPLQAVGLIGQDSDGDYIMAMLEQYHVNRQHVQRTTFAPTSMSQV
                     MTDPSGQRTFFHSPGANRLLDLPAFDQLDNTMKIFHLGYLLLLDSLDMPDDVYGTRSA
                     RLLAQMRDTGFETSLDLVSRKGDPRYQPLVLPALRHLDYLVINELEAGEFSGLEIRLP
                     NGEPHIAHIAAAARELLDAGVRQRVVIHCPEGAWGETPEQGGVWIPSQKLEQREIIGS
                     VGAGDAFCAGFLYGCHERWTLTESIKLAHACARASLLCANAIDGAKTLEELQTEMSD"
     gene            complement(21134..21841)
                     /locus_tag="RHV13_002528"
     CDS             complement(21134..21841)
                     /locus_tag="RHV13_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDV1242053.1"
                     /translation="MKPVVLVVDDDTAICALLQDVLSEHVFTVSVCHTGQEAILRIEG
                     DPDIALVVLDMMLPDTNGLRVLQQIQKLRPTLPVVMLTGMGSESDVVVGLEMGADDYI
                     CKPFTPRVVVARLKAVLRRVGALAVNDEKSAGLSFNGWYLDTMRCQLHNPLQQHVELT
                     QGEYGLLLALAQNARRVLSREQLLAFTHSDSVEVFDRTIDVLIMRLRRKIELNPHQPM
                     LIKTLRGLGYVFAADVH"
BASE COUNT         4815 a         6501 c         6232 g         4496 t
ORIGIN      
        1 aggcaaaaag attattttct ttttgcgttt cctttcaaat gaaaacgatc gtcgtctaaa
       61 atcagcagta cccccgacaa actcagggat tttgtgtata attgcggcct ttttcggcaa
      121 tctgccgttt tttggcgctt ttgccctgct gacttttgag gaaatccaca tgtcattacc
      181 acactgccca aaatgcaact ccgaatacac ctacgaagat aacggcatgt acatctgccc
      241 ggaatgtgcc tacgaatgga acgacgcaga acctgcacag gaaagcgacg agctgatcgt
      301 taaagatgct aacggcaatc tgctggctga cggcgacagc gttaccatca ttaaagatct
      361 gaaggtgaaa ggtagctctt cgatgctgaa aattggcacc aaagtgaaaa acatccgcct
      421 ggttgaaggc gatcataaca tcgattgcaa aatcgacggt tttggtccga tgaaactgaa
      481 atctgagttc gtgaaaaaga actgattgta ttgtgatcgg taagccggat aaggcgctcg
      541 cgccgcatcc ggcaacggtg ccagatgcct gatgcgacgc ttgcgcgtct tatcaggcct
      601 acaaattccc gcaccctccg taggccggat aaggcgttta cgccgcatcc ggcaacagtg
      661 ccagatgcct gatgcctgat gcgacgcttg cgcgtcttat caggcctaca aattcccgca
      721 ccctccgtag gccggataag gcgttcacgc cgcatccggc aacagtgccg actgcctgat
      781 gcgacgctcg cgcgtcttat caggccgcct ctcatctgta taaatttcga actacactta
      841 acaggcttct cttcactgag gtcaccatca tgccgttaag tccctacatc tcttttgccg
      901 gtaactgtgc cgacgcgatt gcttattatc aacgcacatt gggcgcggaa ctgctctata
      961 aaatcagctt cggcgaaatg ccaaaatcag cgcaggacag cgccgagaac tgcccttccg
     1021 gaatgcaatt tcccgatacc gccatcgctc atgccaacgt gcgcattgcc ggaagcgaca
     1081 tcatgatgag cgatgccatg ccgtcaggaa aagccagcta ctccggcttt acgctggtgc
     1141 tcgattcgca acaggtcgaa gaaggaaaac gctggtttga taaccttgca gctaacggaa
     1201 aaatcgaaat ggcctggcag gaaactttct gggcgcatgg atttggcaaa gtcaccgata
     1261 aatttggcgt accgtggatg attaatgtcg tcaaacaaca accaacgcaa taatccgccg
     1321 ggaggcccgc cctcccgcac tgtcatcgaa ttcccgttaa ctcttcatct gttagtcact
     1381 tttaattaac caaatcgtca caataatccg ccacgatgga gccacttttt tagggaggct
     1441 gcatcatgca aacgattatc cgtgtcgaga agctcgccaa aaccttcaat cagcatcagg
     1501 cgctgcacgc ggttgatctg aatatccatc atggtgaaat ggtggctctg ctggggccgt
     1561 ccggttccgg caaatccacc cttttacgtc atttaagcgg tttgattacc ggcgataaat
     1621 ccgccggtag ccatatcgag ctactgggcc gcactgtcca gcgcgaaggc cgtctggccc
     1681 gcgatatccg caaaagccgc gcccataccg gctacatctt ccaacaattc aacctggtga
     1741 accgcctgag cgtactggag aacgtgctga ttggcgcgct cggcagcacg ccgttctggc
     1801 gcacctgttt tagttggttc acccgcgagc agaaacagcg cgcattacag gcgctgaccc
     1861 gcgttggcat ggtgcatttt gcccatcagc gcgtatccac cctctccggc ggccagcagc
     1921 agcgtgtagc gattgcccgc gcgctgatgc agcaggcgaa ggtgattctc gccgacgaac
     1981 cgatcgcctc gctggacccg gaatcggcgc gcatcgtgat ggataccctg cgcgacatca
     2041 accagaacga cggcatcacc gtggtcgtca cgctacatca ggtggattac gccctgcgct
     2101 actgcgaacg catcgtcgcc ctgcgtcagg ggcacgtctt ttacaacggc agcagccaac
     2161 agtttgataa cgaacgcttt gatcatctct atcgcagcat caaccgcgtc gaagagaacg
     2221 cgaaagctgc ctgacatccc catcattgag gaaaacgaat gaacgctaag ataattgcct
     2281 cgctggcctt caccagcatg ttcagcctca gcaccctgtt aagcccggca cacgccgaag
     2341 aacaggaaaa ggcgctgaat ttcggcatta tttcaacgga atcacagcaa aacctgaaac
     2401 cacaatggac gccgttttta caggatatgg agaagaagct tggcgtgaag gtcaacgcct
     2461 tctttgcccc ggactatgcg ggcattatcc agggaatgcg tttcaacaaa gtggatatcg
     2521 cctggtacgg caacctgtcg gcgatggaag cggtggatcg cgccaacggt caggtcttcg
     2581 ctcagaccgt cgcggcggac ggatcgccgg gttactggag cgtgttgatc gtcaacaaag
     2641 acagtccgat caacaacctg aacgatctgc tggcgaagcg gaaagatctc agcttcggca
     2701 atggcgatcc taactccacc tctggcttcc tcgtccccgg ctactacgtc ttcgccaaaa
     2761 acaatatctc cgccagcgac ttcaagcgca ccgtcaacgc cgggcatgaa accaacgcgc
     2821 tggccgtcgc caacaagcag gtggatgttg ccaccaacaa caccgaaaac ctcgacaagc
     2881 tgaaaacctc cgcgccggag aagctcaaag agctgaaagt gatctggaag tcgccgctga
     2941 tcccaagcga tccgatcgtc tggcgcaaga atctgtccga aaccaccaaa gacaagatct
     3001 acgacttctt tatgaactac ggcaagacgc cggaagagaa agcggtgctg gaacgcctgg
     3061 gctgggcgcc gttccgcgcc tccagcgacc tgcaactggt gccgattcgc cagctcgcat
     3121 tctttaaaga gatgcagggc gtaaaaagca ataaaggact gaatgagcag gacaagctgg
     3181 caaaaaccac cgagattcag gcgcagctgg atgacctgga ccgcctgaac aacgcgttaa
     3241 gcgcgatgag ttcggtgagt aaagcggtgc agtaaatcgt aggtcggata agacgccccg
     3301 gcgtcgcatc cgacaatgtg caggcgttga tgccggatgc ggtgcaagca ccttatccgg
     3361 cctacagacc ggagccaaac atgcaaacca tcaccatcgc cccacccaag cgcagctggt
     3421 tctcgcttct gagctgggcc gttgtactcg ccgtgctggt cgtctcgtgg cagggcgcgg
     3481 aaatggcccc gctcacgctg attaaagacg gcggcaacat ggcaaccttc gctgccgact
     3541 tcttcccgcc cgatttcagc cagtggcagg attacctcac cgaaatggcc gtcacgctgc
     3601 aaatcgccgt ctggggcacc gcgctggcag tggttctgtc catccccttc ggcctgatga
     3661 gcgccgaaaa cctggtgccg tggtgggttt accagcctgt tcgccgcctg atggacgcct
     3721 gccgcgccat taacgaaatg gtcttcgcca tgctgttcgt ggttgccgtc ggcctcggcc
     3781 cattcgctgg cgtactggcg ctgtttattc acaccaccgg cgtactctcc aaactgcttt
     3841 ccgaagcggt ggaagcgatt gaacccggcc cggtggaagg cattcgcgcc accggcgcca
     3901 acaaactcga agagatcctc tacggcgtgc tgccgcaggt gatgccgctg ctgatctcct
     3961 attcccttta tcgcttcgaa tccaacgtcc gctcggcgac cgtcgtcggc atggtcggcg
     4021 cgggcgggat cggcgtcacc ctgtgggaag cgattcgcgg tttccagttc cagcaaacct
     4081 gcgccctgat ggtgctcatc atcgtcacgg tcagtctgct ggatttcctc tctcaacggt
     4141 tgcgtaagca ctttatctga ttagcgaggc attgatagct atgcacttgt ctacacatcc
     4201 gaccagctac ccaacacgct atcaggagat agccgcaaaa cttgagcagg agctgcgtca
     4261 acactaccgc tgcggcgact atcttcccgc cgagcagcag ctggcggcgc gctttgaggt
     4321 aaatcgccac accctgcgcc gcgccatcga ccaactggtg gaaaaaggct gggtacagcg
     4381 ccgtcagggc gtcggcgtgc tggtgctgat gcgcccgttc gattacccgc tcaacgccca
     4441 ggcgcgtttt agccagaacc tgctggacca gggcagccat cccaccagcg aaaaactgct
     4501 gtcggtgctg cgcccggcgt ctggtcacgt ggcagacgcg ctgggtatcg ctgagggcga
     4561 gaacgtcatc cacctgcgca ctctgcgtcg ggtcaacggc gtcgcgctct gtttaatcga
     4621 ccactacttc gcggatctca ccctctggcc gacgctgcag cgcttcgaca gcggctcgct
     4681 gcacgatttt ctgcgcgagc aaacggggat tgcactgcgc cgcagccaga cgcggatcag
     4741 cgcccgccgc gcccaggcca aagagtgcca gcgccttgaa atcccgaata tgtcgccgct
     4801 gctgtgcgtg cgcaccctta accaccgtga cggtgaaagc agcccggcgg agtactccgt
     4861 cagcctgacg cgcgccgaca tgattgaatt cactatggag cactgaatgc acgcagatac
     4921 cgcgacccgc cagcactgga tgtccgtact ggcgcacagc caaccggctg aactggcagc
     4981 ccgcctgaac gcgctgaaca tcaccgccga ctatgaagtg atccgcaccg ccgaaaccgg
     5041 cctggtacag attcaggcgc gaatgggcgg caccggcgaa cgcttttttg ccggcgacgc
     5101 cacgctgacc cgcgccgccg tacgcctgac tgacggcacg ctcggctaca gctgggtgct
     5161 ggggcgcgac aaacagcacg ccgaacgctg tgcgctgatt gacgcgctga tgcagcaaac
     5221 ccgccatttt caaaacttat cagaaaccct tattgccccg ctggacgctg accgtatggc
     5281 acgcattgcc gcacgccagg ccgaagtgaa cgccagccgg gtcgacttct ttacgatggt
     5341 tcgcggagac aacgcatgac cctggaaacc gcttttattc ttcccgtgca ggatgcccag
     5401 cacagttttc gtcgcctgtt aaaggccatg agcgagccgg gcgtgattgt cgccctgcat
     5461 cagctcaaac gcggctggca gccgctgaat atcgccacca ccagcgtgct gctgacgctg
     5521 gccgataacg acacgccggt gtggctttcc gcgccattaa gtaacgatat cgtcaaccag
     5581 agcctgcgtt ttcataccaa cgcgccgctg gtcagccagc cggaacaggc gacctttgcg
     5641 gtaacggatg aggcgatttc cagcgaacag cttaacgccc tttccaccgg caccgccgtt
     5701 gcgccggaag cgggtgcaac gctgatttta caggtcgcca gcctgagcgg cgggcgaatg
     5761 ttgcgtctca ccggcgcggg cattgccgaa gaacgaatga tcgccccgca gctgccggag
     5821 tgcattctgc acgaactcac cgagcgcccg cacccgttcc cgctcggcat cgacctgatc
     5881 ctgacctgcg gcgagcgcct gctggctgtt ccgcgaacca ctcatgtgga ggtgtgctga
     5941 tgtacgttgc agtgaaaggg ggcgagaagg cgatcgacgc cgcccacgcc ctgcaagaga
     6001 gccgacgccg gggcgatacc gatttgcctg aactgagcgt cgcccagatt gaacagcagc
     6061 ttaatcttgc ggtggatcgc gtgatgaccg aaggcggcat tgccgaccgc gaactggcgg
     6121 cgctggcgct gaaacaggcc agcggcgata acgtcgaagc aattttcctg ctgcgcgcct
     6181 accgcaccac actggcgaag ctcgcggtaa gcgagccgct cgacaccacg gagatgcgcc
     6241 tcgaacggcg tatttcggca gtttataaag acattcccgg cggccagttg cttggcccta
     6301 cctacgacta cacccatcgc ctgctcgatt tcaccctgct ggcaaacggt gaagcgccaa
     6361 cgctgaccac cgccgacagc gaacaacagc cctcgccaca cgttttcagc ctgctggcgc
     6421 gtcaggggct ggcgaagttt gaagaagata gcggcgcaca gccggatgac atcacccgca
     6481 cgccgccggt ttacccctgc tcacgttctt cccgtttgca gcagttgatg cgcggcgacg
     6541 aaggctattt gctggcgctg gcctactcca cccaacgcgg ttacgggcgc aaccacccgt
     6601 tcgcgggcga gatccgcagc ggctatatcg acgtgtcgat tgtgccggaa gagctgggat
     6661 ttgcggtgaa cgtcggcgaa ctgctgatga ccgagtgtga aatggtcaac ggttttatcg
     6721 accctccaga tgaaccaccg tacttcacgc gcggctacgg gctggtgttc ggcatgagcg
     6781 agcgcaaagc gatggcgatg gcgctggttg accgtgcgtt gcaggctccg gagtacggcg
     6841 agcacgcaac aggcccggcg caggatgaag agttcgtgct ggcgcatgcc gacaacgtcg
     6901 aagccgcagg ctttgtctcg caccttaaac tcccgcacta cgtcgatttc caggccgaac
     6961 tggagctact caaacgtctg caacaggagc agaaccatgg ctaatctgag cggctacaac
     7021 tttgcctacc tcgacgagca gaccaaacgt atgatccgcc gcgccatctt aaaagcggtg
     7081 gcgatccccg gttatcaggt gccgtttggc gggcgcgaga tgcccatgcc gtacggctgg
     7141 ggaaccggtg gcatacaact caccgccagc gtgattggcg aaagcgacgt gttgaaggtg
     7201 attgaccagg gcgcggatga caccaccaac gccgtatcga ttcgcaactt ctttaagcgc
     7261 gtgaccggtg taaacaccac cgagcgtacg gacgatgcca cggtcatcca gacgcggcat
     7321 cgcatccccg aaacgccgct cacggaagat cagatcatta tcttccaggt gccgatcccc
     7381 gaaccgctgc gctttatcga gccgcgcgaa acggaaaccc gcaccatgca cgcgctggag
     7441 gagtacggcg tgatgcaggt gaagctgtat gaagatatcg cccgcttcgg tcatatcgcc
     7501 accacctacg cctatccggt gaaggtgaac gggcgctacg taatggaccc gtcgccgatc
     7561 ccgaaattcg ataacccgaa aatggacatg atgcccgccc tgcaactgtt cggcgcgggg
     7621 cgcgagaagc gcatctatgc ggtgccgccg tttacccgcg tggaaagcct cgatttcgac
     7681 gatcacccgt tcaccgttca gcagtgggat gagccatgcg ccatctgcgg atcgacccac
     7741 agctatcttg atgaagtggt gctggatgac gccggaaacc gcatgtttgt ctgctccgat
     7801 accgattatt gccgccaaca gagcgaggcg aaaagccaat gaatcaaccg ttactttcgg
     7861 tcaataacct gacccacctt tacgcgccgg gcaaaggctt tagcgatgtc tcttttgatt
     7921 tatggccggg tgaagtactg ggcattgtcg gggagtccgg ctccgggaag accacgctgc
     7981 tgaaatcgat ctccgcgcgt ctgacgccgc agcaggggga gattcactat gaaaaccgtt
     8041 ccctctatgg catgagcgag gccgaccgac gtcgcctgct gcgcaccgaa tggggcgtag
     8101 tgcatcagca tccgctcgac ggcctgcgcc gccaggtctc ggcaggcggc aatatcggcg
     8161 agcggctgat ggcgaccggg gcacgtcatt acggcgatat tcgcgctacc gcgcagaagt
     8221 ggctggaaga ggtggaaatt cccgccaacc ggatcgacga cctgccgacc accttttccg
     8281 gcggtatgca gcaacgtttg cagatcgccc gcaacctggt gacgcatccg aagctggtgt
     8341 ttatggatga accgaccggc gggctggatg tgtcggtgca ggcccgcctg ctcgacctgc
     8401 tgcgcggcct ggtggtggag ctgaacctgg cggtggtgat tgtcacccac gatttaggcg
     8461 tcgcccgcct gctggcggac cgtttgctgg tgatgaagca ggggcaagtg gtggagagtg
     8521 ggttaaccga ccgcgtgctc gacgacccgc accatccgta tacacaactg ctggtgtcat
     8581 cggttttgca gaattgagcc ggtgccggat gcggcgtaaa cgccttatcc ggcctacaaa
     8641 tgcgctcccc gtaggtcgaa taagacgcgt cagcgtcgca tccgacaccc gaaccacgag
     8701 gcgaaaaatg attaacgtac aaaacgtcag taaaaccttc atcctgcacc agcaaaacgg
     8761 cgtgcgcctg cccgtcctca accgcgcctc gctcaccgtc aacgcgggcg aatgcgtggt
     8821 gctccacggc cattccggca gcggaaaatc aaccctgcta cgctcgctgt acgccaacta
     8881 tctgcccgac gaagggcaaa tccagatcaa acacggtgac gaatgggtcg atctggtcac
     8941 cgcgccagcg cgcaaagtgg tggaaatccg caaaaccacc gttggctggg tgagccagtt
     9001 tctgcgcgtc atcccgcgta tttcagcact ggaagtggta atgcagccgc tgctcgatac
     9061 cggcgtaccg cgtgaagcct gcgccgctaa agccgcgcgt ctgctcaccc gcctgaacgt
     9121 gccggaacgc ctgtggcatc tggcgccatc gaccttctcc ggcggcgaac agcagcgcgt
     9181 caacatcgcc cgcggcttta tcgtcgacta cccgattctg ctgctcgacg aacctaccgc
     9241 ctcgctggac gctaaaaaca gcgccgccgt agtggaactg atccgcgaag ccaaagcccg
     9301 tggcgcagcc atcgtcggca tcttccatga cgaagccgta cgtaatgacg tcgccgaccg
     9361 cctgcaccca atgggagcct cttcatgatt atcaataacg ttaaactggt gctggaaaac
     9421 gaagtcgtac acggttcgct ggagatgcag gacggcgaaa tccgcgcctt tgccgaaagc
     9481 cagagccgcc tgccggaggc gatggacggc gaaggcggct ggctgctgcc ggggctgatt
     9541 gagctgcata ccgacaacct cgataaattc ttcaccccgc gcccgaaggt cgactggccc
     9601 gcccactcgg cgatgagcag ccacgacgcg ctgatggtgg cgagcggcat caccaccgta
     9661 ctggatgccg tggcaattgg cgacgtgcgc gacggcggcg atcggctgga gaatctggag
     9721 aagatgatca acgccatcga agagacgcag aaacgcggcg tcaaccgcgc cgagcaccgt
     9781 ctgcatctgc gctgcgaact gccgcatcac accaccctgc cgctgtttga aaaactggtg
     9841 cagcgcgagc cggtgacgct ggtgtcgctg atggaccact cgccgggcca gcgccagttc
     9901 gccaaccgcg agaagtatcg cgaatattat cagggtaaat actcgctcac cgatgcgcag
     9961 atgcagcagt acgaagagga acagctggcg ctcgccgcac gctggtcgca gccgaatcgc
    10021 gaatccatcg ccgccctgtg ccgcgcgcga aaaattgcgc ttgccagcca cgatgacgcc
    10081 acgcacgccc acgttgccga atcccaccag cttggcagcg tgatcgccga atttcccacc
    10141 acgtttgaag cagcggaagc ctcgcgcaag catggcatga acgtgctgat gggcgcgcca
    10201 aatatcgtgc gcggcggttc gcactccggc aacgtggcgg ccagtgaact ggcgcagctt
    10261 ggcctgctgg atatcctctc ttccgactac taccccgcca gcctgctgga tgcggcgttc
    10321 cgcgtcgccg atgaccagag caaccgcttt acgctgccgc aggcggtgag gctggtaact
    10381 aaaaatccgg cgcaggcgct taatctccag gatcgcgggg tgattggcga gggcaagcgc
    10441 gccgacctgg tgctggcgca tcgcaagggc aaccacattc atatcgacca cgtctggcgt
    10501 cagggtaaaa gggtgttctg atgatgggaa aactgatttg gttaatgggg ccgtccggct
    10561 ccgggaaaga cagcctgctg gcggaactcc gccagcggga acaaactcag ctactggtgg
    10621 cgcatcgcta catcacgcgc gacgccagcg ccggaagcga aaaccatatc gccctgagcg
    10681 agcaggagtt ttttacccgc gcggggcaaa acctactggc cttaagctgg cacgctaacg
    10741 gtctgtatta cggtgtcggc aaagagatcg acctctggct gcacgccgga ttcgacgtgc
    10801 tggtcaacgg ctcacgcgcc catctgccgc aggcgcgggc gcgctatcaa tcggcgctgc
    10861 tgcccatctg cttacaggtt tcgccagaga tcctgcgcca gcgcctggaa aaccgtggtc
    10921 gtgaaaacgc cagtgaaatt aacgcccggc tggcgcgcgc cgcccgctat actcctcagg
    10981 attgccatac gctcaataat gacggcagcc tgcgccagtc ggtcgacacg ctgctgacgc
    11041 tgatccatca gaaggagaaa caccatgcct gcttgtgagc ttcgcccggc cacacagtac
    11101 gacaccgacg cggtttacgc gctgatttgt gagctaaaac aggcggagtt tgaccatcac
    11161 gcgtttcgcg tgggctttaa cgccaacctg catgacccaa acatgcgcta ccatctggcg
    11221 ctgctggatg gcgaagttgt cggcatgatc ggcctgcatt tgcagtttca tctgcatcat
    11281 gtcaactgga tcggcgaaat tcaggaactg gtggtgatgc cgcaggcgcg cggcctgaac
    11341 gtcggcagta aattgctagc gtgggcagaa gaagaagccc gccaggccgg ggccgaaatg
    11401 accgaacttt cgaccaacgt gaagcgccac gacgcgcacc gtttctatct gcgcgaaggc
    11461 tacgagcaga gccacttccg cttcacgaag gcgctgtaac atgagcctga ccctcacgct
    11521 caccggcacc ggcggcgcac agggcgttcc ggcatggggc tgcgagtgcg cggcctgcgc
    11581 cagagcgcgg cgctcgccgc actatcgccg ccagccatgc agcggcgtag cgaagtttaa
    11641 cgaggcgatc accctgatcg acgccgggcg gcacgatctc gccgatcgct ggtcgcccgg
    11701 atcgttccag cagtttttgc tgacgcacta ccatatggat cacgtgcagg ggctattccc
    11761 gctgcgctgg ggcgtgggcg atccgatccc ggtttacggc ccgccggatg aacagggctg
    11821 cgacgatctg tttaaacatc cgggcctgct tgatttcagc cacacggtgg aaccgtttgt
    11881 ggtgtttgat ttgcaggggt tacaggtcac gcccctgccg ctcaaccact caaaactgac
    11941 cttcggttat ctgctggaaa cggcacacag ccgggtggcg tggctgtctg acaccgcagg
    12001 cctgccggaa aaaacgctga aatttttacg caataatcag ccgcaggtaa tggtgattga
    12061 ttgcagccac ccgccgcgcg aggatgcgcc acgtaatcac tgtgatttaa acaccgtgct
    12121 ggcgctgaat gaagttatcc gctcgccgcg ggtgattctg acacacatca gccaccagtt
    12181 tgatgcgtgg ctgatggaga atgcgctgcc gccgggtttt gaggcggggt ttgatgggat
    12241 ggagattggg gtggcgtgat gagagggaat gtgcgcgctg gccccctcac cctagcctac
    12301 ctcatcgccc atactcatct tccattctcc gctcttcatc ctccagctgc cgacgctcct
    12361 gatcaagctg gcgctggcga tcgtccagtt gcctgcgacg atcttcaaac tggcggcggc
    12421 ggtcgtcata ttgtctgcgc cgatcgtcgc tcacttcacg ctgccagcca tcgtcgcgcg
    12481 aatcttcata gtctcgccca cggtcagggt tataagcatc gttaatcgcc tgctgaatat
    12541 tgccaatggt gtcgtcgata atatcggcct gggccggtac ggtggtcagc gtgagcaggg
    12601 tgaaaaaaaa gagcgtggag tggcgtttca taaactcggc ctcgcaacaa agttaataca
    12661 aggtaggcaa aggtgcctgt tacgagtaga ggaggattct caattctctt gccccagcgc
    12721 ctgcgccagc tgcgcacggg taaagggttt gcgcaacagc gccacgtctg gcagcgcggg
    12781 gttatggctg gggcgtaaat cctgaccgct gatgagtagc agcgtcaggt gcgggtaaag
    12841 tttgcgggcc gcattgacca cttctgcacc gctcatgccg ccggggagca ttaaatcgct
    12901 gataaaaata tcgatttctg ctgatgccgc cagcagatcc agcgcctgtt cgccgctgct
    12961 tgcttccagc gtcaaatagc ccagcaggtg caactgttcg cagatggtct ggcgcaccgc
    13021 ggcttcatct tccagcacta acaccagttt atcgccgctg acagaagcct gttctactgc
    13081 cgctggcgat aggtttttcg ccgccgtcag cgcgcgggga agctgtaatc tgaccgttgt
    13141 cccctgcccc ggcgcgcttt caatttcaac gcgcccaccg gactggcgca caaaaccata
    13201 gaccatcgac aaacccagcc cgctgccgct gcccgtctgt ttggtggtaa agaatggctc
    13261 aaatacctgg cttttcaccg cctgcgacat accgctgcca tgatcgatga cctccagtgc
    13321 caccatgtcc tgacgctgcc cactgctgcg agtaacgcgc tgattccagg tacggatttt
    13381 gatgattccc gtctgccctt ccatcgcgtc acgggcgttc atcaccaggt tgatgatggc
    13441 attttccagt tggctgatgt caatccacgc gggccacgcc ggagactgcg cttcaatctc
    13501 cagcgccagc gttgccggta aggagtggcg cattaattcg cttaactctt ccagcaatgt
    13561 tttcagttca acggcttgcg gatgtaagga ctgcttgcgg gaaaacgcca gcagtcgttg
    13621 tgtcagtagc gccccacgtt cggcagcttg cagcgcccgc tgtattcgcg gcgcgtctgg
    13681 cgagtccggc ttcaccagct ccaggctgcc aataatcacc gccagcagat tattaaaatc
    13741 atgggccagc ccgcccgtca gttgcccgac cgctttcagt ttctggctgt gtaatagcgc
    13801 ctcttccaac ccctgccgct cagtttgggc aatctccatc tgcgtggtct tctcttttag
    13861 cagccgtgag gttcgttcga gtgactcggt attgcgggca aaaacgttaa aggcgcgcgc
    13921 cagttcaccc aactcatcac gccgctgtaa cgccggaaaa gtgacgtccg cttcgccgtg
    13981 cgccaggcgg ctcatggcgc gggaaatcgc cgtcaggtta gtgccgaggt tgcgatagat
    14041 ataccagccg gcaaacgcgg tgatcaccac cgctaacagg gcgaaaatca tgatcaccct
    14101 ctcgccggag cgcaaatcct ggtaactttg cgccacacgc tgaccggaga gcgtcgccac
    14161 ctgttcggcg taacggttga tgtcgccatt gaggatcgcc accagcgctt tgatgtgaaa
    14221 catataccag ctgattgcca gatcgctttg ctccagagct gccgagagtg gcgccagttt
    14281 acgcagttca cgattaaaat cattgagtag cgtgttaacc aatggcgtat cgctgtgggt
    14341 cggcaacgca gacattaccc cgaccagttg atgaataatc gcctgcggcg cgggggtggc
    14401 aatagcggca acaatcagtc ggttcatctc gttaagcaaa atatcatctt gcgcgtgagt
    14461 gactttttgc agatgccgca gataacttaa gttttgatac agcgaactta acagcgcgtt
    14521 gcgctccaga tgccgccttt gcccgcgctc cagcataccg ccgacgctgg tttgcagttc
    14581 gttgctacgc gtgatgattc gcgtcaccag cccggcttcc tgctgcgcta aaggcgcgtt
    14641 cgccagttgt gcaagcgagc tttttaatgc ccgctgtgtc tcctgcaacc gctcggcttc
    14701 gcctttatat tccagtgctc ccaccacctg cgacaatcgc accgccgccg ttgccacgtt
    14761 tgccgtgtcg cgcgccagat tcaggctgcc ggtcatatca tccaccgttt gctgctgcac
    14821 cagctcctgc atctggctgg catgacgaaa gcccattacc gccacgccgc tgaccatcaa
    14881 cgtcaccgcc accaccagca aattcaaaag cagcaatcgc ccgcgcgcgc tggcaaaaaa
    14941 gtgacgtccg gggtgcgcca tatcacgctc cgtgaagaaa aatcgcaata tgacaaatat
    15001 gaacggcttc tgacattttg catttacttt cctgtgatgg agtgctgata tcgacaacct
    15061 cgcaggagtc caccatgaac agcacgccag ttctggagat gcgcaatatt gccaaagcct
    15121 tcggcaaatt ttatgcgctc aaaggggtgg atttgacggt ctaccctggc gagatccatg
    15181 ccctgatggg ggaaaacggc gcgggaaaaa gcacgctgat gaaggtgctg gcgggggcgt
    15241 ataccgccac cagcggcgag atcctcatag acggcaaacc ctttcacatt cgcacgccaa
    15301 aagatgcctt aagcgccggt attacgctga tttatcagga gatgcagctg gcaccgaatc
    15361 tgtcggtggc agaaaatatt tttctcggca gcgagctttc ccacggcggg ctggtgcagc
    15421 gtaaagagat gctagtgcag gcgcaaaaag tgatcgaccg cctcggcgcg cagtttaacg
    15481 ccagcgataa ggtcatgacg ctgaccattg ccgagcaaca gcaggtcgaa atcgcccgcg
    15541 cactacatcg caacagccgc attctggtga tggacgaacc caccgctgcc ctctcctccc
    15601 gtgaaactca ccgtctgttt gaactgatta tgcggctgcg cgatgaaggg atggcgatta
    15661 tctacatcag ccaccgtatg gcggaagtgt atgagctttc cgatcgcgtc agcgtgctgc
    15721 gcgacgggca atacgttggc agcctgaccc gcgataacct caatgccggg gagctggtgc
    15781 ggatgatggt cggcaggcca ctgagcgatc tgttcaataa agagcgcgat atcccgctcg
    15841 gtaaagcccg cctgaatgtt caccacctga ccgacggcgg caaagtccag ccgagtagcc
    15901 tgctggtgcg ttccggcgaa attgttggcc tcgccggact ggtgggtgcc ggacgttccg
    15961 aactggcgca gttgatcttc ggcgtgcgga aagcgacagg cggaatgatt gaagtcgatg
    16021 gtgaaccggt ggtgatccac tccccgcgcg aagccatcga tcttggcatt ggttttctca
    16081 ccgaaaaccg caaagaacaa ggcttattcc ttgaaatggc agcagccgaa aacatcacca
    16141 tggcaaccct ggagcgcgat gcccgctggg gaatgctcaa tcgcaaaaaa gcgcaaacca
    16201 tttccgatga cgccattaag ttgctcaaca ttcgcgtgcc tcatgcccag gtacgcgcgg
    16261 gcgggctttc cggcggcaat cagcaaaaac tgttgatctc ccgctgggtg gcgattggcc
    16321 cgcgcatttt actgctcgat gaacccaccc gcggcgtgga cgttggcgcc aaaagtgaga
    16381 tctaccggat catgaacgag atggcgcgca agggcgtggc gatcctgatg atctccagcg
    16441 aactgccgga gatagtggga atgagcgatc gcgtctatgt gatgcgcgaa ggcagcattg
    16501 ccggtgagtt aaacggcaaa aacatcaccc aggaaaacat tatgacctta gcgactggcg
    16561 tcaacgacgc ccattcccag gcggtaacct catgacgacc tctgcaaaaa cctctgccaa
    16621 aaaaatgctg atgagcgatc tgatgcaaac agtcggcatc ttgcccattt tgattctgat
    16681 cgtggcggta tttggcttta tcgcgccaaa cttttttacc gaaagcaacc tgctgaatat
    16741 tacccgccag gcatcgatca acattgttct ggcggcgggg atgaccttca tcattttgac
    16801 cggcgggatc gatctttccg tgggttcgat cctcggcacc acagcggtgg cggcgatggt
    16861 cgtctccctt agccctgagt tcgccgtgct gtcgatcccg gcagcgctga tgctcggcct
    16921 gctgctaggg ctgttcaacg gcgcgctggt ggcctttgcc ggattgccac cgtttatcgt
    16981 tacgcttggc acctatacgg cgctgcgcgg ggcggcgtat ctgctggcgg acggcaccac
    17041 ggttatcaac tccaacatta atttcgagtg gataggcaac aactatctcg ggccggtccc
    17101 atggctggtg gtgattgcgc tggcggtgat cgtggtgtgc tggtttattc tgcgccgcac
    17161 cacgcttggc gttcatatct acgccgtcgg cggcaatatg caggcggcgc gtcttaccgg
    17221 cattaaggtc tggctggtgc tgctgtttgt ttatggcatg agcggcctgc tttctgggct
    17281 tggtgggatt atgagcgcct cacggctgta cagcgccaac ggcaacttag gcatgggtta
    17341 cgaactggat gccattgcag cggtgatcct cggcggcacc agttttgtcg gcggcatcgg
    17401 caccatcacc ggcacgctgg tcggcgcgct aattatcgcc acgctcaaca atggcatgac
    17461 cttaatgggc gtctcttact tctggcaact ggtgatcaaa ggggcggtga tcatcattgc
    17521 ggtgctgatc gacaaatacc gtacccgaca ccatcaaagt gcataaacac atgaggaata
    17581 cagcatgaga ttgaagccct tagtgaccgc gctctgtgca ggcgcgctgc tggccgcagc
    17641 accgttcgcc caggcgaaag aactgaaatc aataggcgta acggtgggcg atctggcgaa
    17701 cccgttcttc gtgcagatca ccaaaggcgc ggagctggaa gcacgcaaac tggcaggcga
    17761 taacgtcaaa gtgacgctgg tttccagcgg ctacgatctg ggtcagcagg tgtcgcagat
    17821 agacaacttt atcgccgcta acgtcgatat gattatcctc aacgctgccg attcaaaagg
    17881 gatcggcccg gcggtgaaac gggcgaaaga tgccgggatc gtagtcgtcg cggttgacgt
    17941 agcggcagaa ggtgctgatg ccaccatcac ctccgataac acccaggcgg gcgaaatggc
    18001 ctgtaaatac atcaccgatc gcctgaaagg caaaggcaat gtggtgatca tcaacggccc
    18061 gccggtatcc gccgtgcaaa accgcgtcga aggatgccag actgaattta aacgccatcc
    18121 tgacatcaaa gtgctctctg acaaccagaa cgcgaaaggc agtcgtgaag gcgggctgga
    18181 ggtaatgacg ggactgctgg cggcaaatcc gaagatcgac ggcgtatttg cgattaacga
    18241 tcccaccgca atcggtgccg atctggcagc aaaacaggcg caacgtaacg agttctttat
    18301 tgtcggtgtg gacggaagcc cggacgccga agaggcgctc aagcgtgaga actcgttgtt
    18361 tgtcgctact ccggcgcaag atccccaggt gatggcagcg aaagcggtgg aaattggcta
    18421 cgacattttg cagggcaaac ctgcgccgat agagccggtg ctgatcccgg tgacgatgat
    18481 cgataaaaac aacgtcggca attacaaagg ctggacggtg aaataagtcc acacgccggg
    18541 cggcgcagct gcccggtcac cgctaaggag aacgctttcg atgaaacgct ccgctataaa
    18601 cgacattctc ggccatacac ggcaattctt ttcgcaacat gacgtacatc tgccgccctt
    18661 tgccagtttc tcgcctgctc aatggcagca gctcgacacc gccgcgtggg aggaggtttt
    18721 tgatcttaaa ctcggctggg atgttaccgc ttttggtcgc aacaactttg cagcacacgg
    18781 gctgacgctg tttaccctac gtaacggctc agcaaaagga atgccgtatg tgaaatgtta
    18841 cgccgagaag atcatgcatg tgcgcgatgc gcaggtcacg ccaatgcatt ttcactggcg
    18901 taagcgtgag gacattatca atcgcggcgg cggtaattta attgttgaac tgtggaatgc
    18961 cgatagtaac gagcaaacgg ctgatagtga cattacggtg gtgatagacg gctgccgcca
    19021 gaaacatact gcaggcactc agttgcgcct gtctcccggc gaaagtatct gcctgccgcc
    19081 cggcctgtat cacagctttt gggcggaaac cggctttggc gatgttctgg ttggcgaagt
    19141 ctcttctgtc aatgacgacg accacgataa ccacttttta cagccactgg accgctacaa
    19201 cctgattgac gaagacgaac ctgcacagtt ggtattgtgt aacgaatacc gccagttccg
    19261 ctgaataagg agaacgttat gccattgatt tctctcgctg aagggctggc acatgcccgc
    19321 gaacatcact attcgttagg tgcgtttaat gtgctggact cgcacttttt acgcgccttg
    19381 tttgctgctg ccagacaaga acgttcgccc tttattatca acattgccga agtacatttt
    19441 aaatatatct cgctggattc cctggtcgag acagtgaaat ttgaagcggc gcgtcatgcc
    19501 attccggtgg tgttaaatct cgatcacgga ctgcattttg aatcggtggt gcaagcgttg
    19561 cggctgggat tcagctcggt aatgtttgat ggctccacgc tggggtatga agaaaatgtc
    19621 cgccagacgc gggaagtggt gaaaatgtgc catgccgttg gcgtatcggt agaagctgaa
    19681 ctgggcgcgg tgggtggcga cgaaggcggt gcactctatg gtcatgccga tgaatccctg
    19741 tttaccgatc cgcaactggc gcgtgatttt gtcgaccgta ccggcattga cgcgttggcc
    19801 gtggcgattg gcaatgcaca cggcaaatat cgcggcgaac cgaaactcga tttcccgcgc
    19861 ctcgacgcta tccgccagca ggctgctatc ccgctggtat tacacggcgg ctcagggatt
    19921 agcgatgccg atttccgccg cgccatcgaa ctgggtatcc acaaaatcaa tttctacact
    19981 ggcatgtcgc aagcggcgct ggctgccgtt gaggcccgaa tggcgcaccg tcagccgatg
    20041 tatgacgagt ttgccgagtt actgctggca gtggaagccg ccatcagcga tacggttgca
    20101 caacagatgc gtatttttgg cagtgcgggg caggcatgat ggagcgtaaa gggattatcg
    20161 ccgcaggcaa tatgttggtt gaccacgtcc atcagattat ccagtggcca gagcgcggct
    20221 ggctggcgga gatcacccac agcgaacgct ccaccggcgg tgcgccgctt aacgtcctgc
    20281 tgacgctggc gaaaatgcgc accgggctgc cgttacaggc ggttgggcta atcggtcagg
    20341 acagcgacgg cgattacatc atggcgatgc ttgagcaata tcacgtcaat cgtcagcatg
    20401 tgcagcgcac tacctttgcc ccgacgtcga tgtcgcaggt gatgacagac ccaagcgggc
    20461 agcggacgtt tttccattcg ccgggtgcaa acagactttt ggatcttccg gcgttcgatc
    20521 agctcgataa caccatgaag attttccatc tcggctattt actgttgctc gacagcctgg
    20581 atatgccgga cgatgtttac ggcacccgta gcgcacgcct gctggcgcaa atgcgtgata
    20641 ctggctttga aacctcactc gacctggtct cccgtaaggg cgatccgcgt tatcagccgc
    20701 tggtgctgcc cgcgttgcgc catctggatt atctggtaat taacgaactg gaggccgggg
    20761 agtttagcgg gctggaaatt cggttaccca acggcgaacc gcatatcgcg catattgctg
    20821 ccgccgcgcg tgaactgtta gatgcgggcg tgcgccagcg ggtggtgatc cactgcccgg
    20881 aaggggcgtg gggagagacc ccagaacagg gcggcgtgtg gatcccttcg caaaagttag
    20941 aacaacggga aattatcggc agcgtcggcg cgggcgatgc attttgtgcc ggatttttat
    21001 atggctgcca tgaacgctgg acgcttaccg agagcatcaa actggcacac gcctgcgcgc
    21061 gggcaagcct gttatgcgcc aatgccatcg acggggcgaa aacgctggaa gagctgcaaa
    21121 cagaaatgtc ggattaatgc acgtcggcag cgaagacata ccccagaccg cgcagagttt
    21181 taatcaacat cggctgatgc gggttgagtt cgattttgcg tcgtaagcgc atgattaata
    21241 cgtcgatggt gcgatcaaac acctccacgc tgtcgctgtg ggtgaacgcc agcaactgct
    21301 cgcggctcaa tacccgacgc gcgttttgcg ccagagccag taacaagcca tattcgccct
    21361 gagttaactc gacatgttgc tgaagcggat tgtgcaactg gcagcgcatg gtatcgagat
    21421 accagccatt gaatgacagc ccggcgcttt tctcgtcatt aaccgccagc gcaccaaccc
    21481 gtcgcaacac cgctttcagt cgtgcgacca ccacccgcgg ggtgaacggt ttgcagatgt
    21541 aatcatccgc gcccatttcc agcccgacca ccacatcaga ctcactgccc atgccggtca
    21601 acatcaccac cggcaatgtc ggacgcagtt tctggatttg ctgcaatacc ctcaggccgt
    21661 tggtatcggg tagcatcata tccagcacca ccagcgcaat gtcaggatcg ccctcaatac
    21721 gcaatatggc ctcctgtccg gtatggcaga ctgaaaccgt aaagacatgt tcacttaaca
    21781 cgtcctgcaa cagtgcgcag atagccgtgt catcatccac caccagcacg acgggtttca
    21841 tggttacact ccgcatcatt catctgatta atcattgtgc gtacttcttg tcgaagtgaa
    21901 agctgaaagt aatataaata ataaaaatat aagtcaggtc acattattga tgatagtaat
    21961 aaatatgccg tacttttccc tcttcagcac aaatgcctaa taaaatggat cgttccgctc
    22021 acacttccac ttattaaagt cgca
//
LOCUS       DAPGOF010000077        22039 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.077, whole genome shotgun sequence.
ACCESSION   DAPGOF010000077 DAPGOF010000000
VERSION     DAPGOF010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22039
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.077"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..560)
                     /locus_tag="RHV13_002529"
     CDS             complement(<1..560)
                     /locus_tag="RHV13_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDV1242054.1"
                     /translation="MLIGYVRVSTNEQNTALQRNALESAGCELIFEDKASGKKAERPG
                     LKKVLRMLSRGDTLVVWKLDRLGRSMRHLVVLVEELRDRGINFRSLTDSIDTSTPMGR
                     FFFHVMGALAEMERELIVERTRAGLDAARAEGRIGGRRPKYQEETWQQMRRLLEKGIP
                     RKQVAIIYDVAVSTLYKKFPASSFQS"
     gene            688..969
                     /locus_tag="RHV13_002530"
     CDS             688..969
                     /locus_tag="RHV13_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242055.1"
                     /translation="MCDFTIMLLSILGGVHSFLNGVREKRYEASCRQLMAECIAAVLA
                     GFIGMYFAEYKGMDESLQNCVTIICSINNRLILEKLQRIIDSHLNRNAS"
     gene            1037..1366
                     /locus_tag="RHV13_002531"
     CDS             1037..1366
                     /locus_tag="RHV13_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HDV1242056.1"
                     /translation="MLDTQELAPVAIALLLSVIGGIGTFLMDVRDGRQSGNLLGLVTE
                     IFVAVTAGAVAYLLGQHEGWELSITYLMVTIASNNGHEVISGMKRVNIDSILNVLTSL
                     VKKGGGK"
     gene            1363..1806
                     /locus_tag="RHV13_002532"
     CDS             1363..1806
                     /locus_tag="RHV13_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HDV1242057.1"
                     /translation="MIGWGVCVLALALADRYLLKRKDITHLELGDVEIKPGFIRVPFK
                     YRSKFPFLRGATVRYWIRDVQKPTTVIEGEQRCLTSAEQGENSEWLYIPTEYMGKGER
                     LWHFNVMVTHGDSFINPLYRIFPVTQQIRRSYVINLAQDVSDDEK"
     gene            1793..2395
                     /locus_tag="RHV13_002533"
     CDS             1793..2395
                     /locus_tag="RHV13_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002433476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="odaE"
                     /protein_id="HDV1242058.1"
                     /translation="MTKNKYATVDFDQVNEKGLKSLIAAINKTGVTVIEVDSSNRATT
                     KDGVKVKTAKLVLNDGQILAIQVNDTGDISSVKLNGKAIPNAQSPDIKTLGTVMGQAA
                     RKNSAKFQKSLIAKAKRVANPVDKKPAVKSNFQRLQEAKQRNAQVVAAYKSAQNSVSF
                     NQQQITDLRAKLDKETGRLNNEKARNGELKRRLKQLKAGN"
     gene            2397..4316
                     /locus_tag="RHV13_002534"
     CDS             2397..4316
                     /locus_tag="RHV13_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242059.1"
                     /translation="MEQFNINKGVTLKPGLDVLPPPVTDDEYRALMAGEDRYLMTESN
                     TLEEIEATFFYDTPIHWCATDLLEAISSTRLQLHRTMQAFVRALNQKLNGTGISAGSD
                     KTGDVAQSGARAIGGAEIGRARNVNGLPVLPAIIPLSDGQTISILFHSPTAENRITNS
                     DTLVAFQFLLNKKDVTHTVAPMSGRDMTLAQVTMKLANLAEKNSAKFQRAQKKKKALV
                     DEITQLQADSDQKEDAMSDLADQVAAVEGQKADLEQKINAVASEADSLYEENERLQTE
                     IDQLNRTGGRDTIAPAGMTGGHSRALTDRLASIKNRMHMDGEATLSNGASMKQFIGDG
                     EGYIQLTDPDGSVYMIKAKSIQGVDMADAIGKLFKAYKAGNVSEYLVQPEEHKPENVE
                     PEPAEDTGSSSPEPEVSVGAYRYALQMRPAAPGAIPEGNKAILPRPDEGDPYYEYARY
                     GIATYDTPLSDQQMSEYDLKLLPREDSFDFLAKTLTNGPFGKYAQKALELATSSPDEF
                     RVMLKTQFQKTFPNIAFPGGAGTEKMVQSMINALQAEVGEITQPEPAPAQPDETVSEA
                     DAEANKAIEYLNNVMDMQSTDMAEIRNARGNIREAIVALQAAGRFEENEELVNGAARH
                     LADLLVAIQKAGVAA"
     gene            4313..4678
                     /locus_tag="RHV13_002535"
     CDS             4313..4678
                     /locus_tag="RHV13_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ddrA"
                     /protein_id="HDV1242060.1"
                     /translation="MTLSAIELMDLSDKLDALMSKAATASGMELLDISDEIDQIMQQM
                     GYGASGGGSGDEKQPSEHDGVPKLVADFLADKFVDQSTDAFIGTLQDLSQYVGTYIDL
                     DQVKQHTAAWIAANIKEAA"
     gene            4691..7678
                     /locus_tag="RHV13_002536"
     CDS             4691..7678
                     /locus_tag="RHV13_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242061.1"
                     /translation="MSLSDQVVMATSIETLIELLKNLPDYGRVSYVVTAKGDEVKTAF
                     DIVDASALLVSNTLDGKINPDYPQELQPRDRTRASSLLQVNQISKDLRPAQLTDSGLS
                     SHGAPIIGEDNAVESGNGRTMGIIKAYQDGNADRYREYLIEHATEFGIRPEKVESMTA
                     PVLVRRRLTKVDRVQFAKDSNISDLQEMAASEKAFVDADSITPAMMALFNPSESGDLL
                     SRSNDAFIRGFMTQVGATQAAGLVTEDGRPTRQLVDRIQNAIFAKAYKDARLVRMVAE
                     EPDPDMRNVLTALNAAANDFVQMQALSGEAHKQAVTTIVDGIETADSLDKKALAALKD
                     AVDLVRQSKESGQHITDVIAQGDMFSETAPEVKALALFIVANNRSAKRMATAFKLMAQ
                     RINDELQHQGQALGDMFGGGDVSLQDILRQVSQELEYEGMQGISGGLFESVSGGSYNG
                     VAPYTSLLLHRASGIKDIIHLIRLLSRTDPQDEQLVQVLAHFVRMPVADVKKWCRLFG
                     ISNSLLRGLLNHASSLGRDGFDEIAQAIKNGDMPPAIDWFSIRPTRVKAFLSAAHSAS
                     PLAEMVQRLSLIFTDHTALGDLTLDEMKEASIQWADQQNEVNSDFLPAFRKAVSKADD
                     ARGILKAFKALQSQVNKHVGDIDGVTAEGRDILKEHGITPEFIDEIRTDMQREVVSSL
                     QIVARALADANPKSAAIVNRVIGDIEASEGMGALKLFLSRAFNPNGNILPGIIGEAKK
                     YVSEEELEQLDQLLKRFSYNPQTRWQMNQRSMGSVHEKVLSAMNSAIANSSVSEEKAL
                     EWADSFITEEVEEARAGQNGGIDLRKELADIYRLTGGKISTLSKVVHHKGRAYANLNG
                     VVAVNLNDENASALWHELGHHLEYSNPGLLEKARSFLKANVEGDKPSFVNIGGRGKPE
                     WCFRSRLSNIYMAKVYPPVSVSNSGKIRQKSPTISKTSATEVFSMALQLYHDKEAAAA
                     SLMNGDGLLELLLGVAKELNNAD"
     gene            7668..7976
                     /locus_tag="RHV13_002537"
     CDS             7668..7976
                     /locus_tag="RHV13_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242062.1"
                     /translation="MQIKIAAPLGGDAIIEFDDNEEVSGRLSIISGDITEDMIAEAIA
                     GANPNSYMGFVNTLDAPASDVLRTLYLYAGWFVDWPAVDGGDEDDDDDFGDHIDQIIY
                     "
     gene            complement(8008..9102)
                     /locus_tag="RHV13_002538"
     CDS             complement(8008..9102)
                     /locus_tag="RHV13_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242063.1"
                     /translation="MINRIQNESAWLALIEQYKDNWELAAKELLDIELTPHQAKIINA
                     IKNTGAKVTATTPHGIGETSIVAVISILQTILYSCSRTVVVSPAINDSRKTIIDYMFR
                     YWERVTQKHPFLGNHFRMHPDKGLVHISETWGCVYITYCLNNEVSLAGFTGPHDLFIV
                     INSAEISDRAHAVITGNLTNYDSRLLLLSKPSEREKGYFYDSHHRLAHSKNNPAGFFT
                     AITLNTEDSPLVSEAYLEFKAKEFGGRNSDEYRRLILGKFPGIRELMEKSSVPRNMHF
                     TMTDGSKWVVPTIVIAKHHAKHHAQKHNSSTLDWLKAYTIPLFSANHNAIAEWAKQIP
                     WQDVAEEAFVEKPPRDLHEHCWLTSEKSFS"
     gene            complement(9095..9889)
                     /locus_tag="RHV13_002539"
     CDS             complement(9095..9889)
                     /locus_tag="RHV13_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242064.1"
                     /translation="MTAQNTKTIQYRLRNGQSVEVTINNDGVPGEKVSISDLAIEKTI
                     MCHLGFTEEVSKKHGVAIWRTMDTGMRRFITARTPGMTMMDLMQIAPLFECEPLDVFS
                     NPVICQQLYGEMKLAVTPIVLHEGSLAGVWKVERISSYMPFHVHVNGVITGENQPVSV
                     TKSDLKRAILEASCRVIGLGKQSYVCFPAGPEGQAEILAMDADLLWQIEFMIGKSIIR
                     AEELDQYITCTMTDEVKSVAIAKARNLCRAALTELRENTTEEVESD"
     gene            complement(10087..10575)
                     /locus_tag="RHV13_002540"
     CDS             complement(10087..10575)
                     /locus_tag="RHV13_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDV1242065.1"
                     /translation="MAQRGVNKVILIGTLGQDPEIRYIPNGGAVGRLSIATNESWRDK
                     QTGQQKEQTEWHKVVLFGKLAEIASEYLRKGSQVYIEGKLKTRKWTDDAGVERYTTEI
                     IVSQGGTMQMIGARRDDSQSSNGWGQSNQPQNHQQYSGGGKPQSNANNEPPMDFDDDI
                     PF"
     gene            10745..11302
                     /locus_tag="RHV13_002541"
     CDS             10745..11302
                     /locus_tag="RHV13_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HDV1242066.1"
                     /translation="MKGKTAAGGGAICAIAVMITIVMGNGNVRTNQAGLELIGNAEGC
                     RRDPYMCPAGVWTDGIGNTHGVTPGVRKTDQQIAADWEKNILIAERCINQHFRGKDMP
                     DNAFSAMTSAAFNMGCNSLRTYYSKARGMRVETSIHKWAQKGEWVNMCNHLPDFVNSN
                     GVPLRGLKIRREKERQLCLTGLVNE"
     gene            complement(11594..12613)
                     /locus_tag="RHV13_002542"
     CDS             complement(11594..12613)
                     /locus_tag="RHV13_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000132944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head processing protein"
                     /protein_id="HDV1242067.1"
                     /translation="MTDVLKTVTDRFCLYSNARKGRQNGRQYVLSAVKTMLESKETQE
                     GLRLGELFGYYGHGRRQLTGKLEVPETSVIMVEGRPVVIDNVPACRTVAISVDDNGIV
                     THTQEILNTEPGKIVAAMIESRAGGWSWATGGRESGKIAVTTSFHGVDYVTTPNYISL
                     DHPASAGMFESADSKSLLAESLAAHGYSDESVQAVISHYGKMAELEMMVEATERTAEL
                     ETALLESQGRHLEAMAKIADAEARIALLEKTAGIRDDVLAAMQDELDNLPIFVSAAQK
                     DAFRLKEPGDAKIVATLFESLIKVGARNLPVTKKIKEVPQAANVQAPREASIITFNNS
                     INPFK"
     gene            complement(12606..14315)
                     /locus_tag="RHV13_002543"
     CDS             complement(12606..14315)
                     /locus_tag="RHV13_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDV1242068.1"
                     /translation="MADNKITLSSVRKALAGVFKDNGERDNILLSALAVHGGSGYLFS
                     RAGAPVQLSGFLGGQPGDSGMAGDGLVDGSRFIFDEVQLPEDRLQRYPLLEEMAVYST
                     IATALNIHITHALSFDKKTGQTFSIVPVHNGNDSDYDDAQALCDELMNDIGRTINKEV
                     AGWAFIMSVFGVAYVRPYAKEGIGITSFECSYYTLPSFIKEFEVSGNLAGFSGDYLKD
                     ASGKMVFADPWTIIPMKIPYWRPKSNLMPVHTGHKAYSLLDNPEERTPIETQNYGTSL
                     LEYAYEPYMNLRSAIRSLKATRFNASKIDRIIGLAMNSLDPVKAADYSRTITQTLKRA
                     ADLMEKRARGANNMPTVTNTLLPIMGDGKGQMTIDTQTIQADINGIEDILTYMRQLAA
                     ALGLDYTLLGWADQMSGGLGEGGFLRTAIQAAMRASWIQQGVDEFILRAIDIHLAFKY
                     GKVYPEGDRPYKIEFHSVNTALQQEHNDNRDSQANYATIVTQILDAVSNNSVLANSDA
                     FKRYLFSDVLEIDEKISEALVNELKAKSEDDDHLMDSIIKTPPQELAQILESVFKEGN
                     DND"
     gene            14391..21158
                     /locus_tag="RHV13_002544"
     CDS             14391..21158
                     /locus_tag="RHV13_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HDV1242069.1"
                     /translation="MNKLSIGVFRCSSVSEILKYIRAITSHRAPIKYGVEKVEGKSYD
                     RLRREANQKAIDLLNSLVDGATLTDEQRQILAGYTGEGGIGGSVSEYYTPKPIAEGVW
                     EIMKLYGADVGNTLEPSAGTGVFNETKPVGTVMTATEISSVSGRINQLLHPEDSVQIS
                     PFEQLAVSTPNDSFDHVVGNVPFGGRDNTRNIDKPYAEETDMGSYFMLRMLDKIKPGG
                     FMCVIVPPSIVSGSNMKRLRLRLSRKAEFLGAHRLPTGTFDANGTSTVVDVVLMRKHP
                     AEMAEKIPLVDESTLESANVLWPTFISGKWFEKDGRRFVHGTQEKGFQGRIEVRADGQ
                     IDNQALKAKLIHRFESRIDWSLLDMAEPSPTADVVGEGEMRLINGVWQKYAGGRWIEA
                     DAGKELKIDAASYGADSWEALQRNLTTTEGRLGMTFTQMANVRDKYTTSISDDMVQLV
                     DWINSQPEKYRERLYRGAMIGRMLIEYQDMKAAGHSAEQIEQQRLSLVSRLQAEIDRF
                     GNPGRGPIAKLSGSGARAWFAFRGAIKLDGTISDELTGKLVTHDSSASYDSTSYQDTL
                     RYLYSDLTRDPIQLDDFRLAFTGELPASDEELLNLLASTPGIAVSPYGGIVPFARATS
                     GDINEIVAPKQEFLATLPDGPVKNNVLNQLAAIEEKRIKTPAENIRFKLNSRWFDRSV
                     ILEFLQENGYPDLRYVQSVQLEGDEMVSDTYHGGDGLFVGHRYGVVQRKDKETGEIRY
                     EWDRKSGENATGFPAQLEKYLNGARIGGKDSATANGYREQMALLEDQFNKWIKTHDRY
                     DELVAKYNDVFNSNIPYEHSGDPLGLKGLSGKRQPFDYQNSEVRRLSEDGRGILGFGT
                     GLGKTTTALALEAFNYENGRSTRTAYVVPKSVLENWYYEAKEFLSEEAFSNYLFVGLD
                     VLMDGDQIRQVPVLDENGKPVVGADGTPVMRDALKLADEATITARMNAIPHSNYRAVV
                     FTKEQYARIPLRDDTVDEHAQDMLYDFVAAGRVASAMDSDSHRKEAARRRVLSEYSDT
                     GTEKAEKYPYFEDMGFDSVIADEGHNYRNSYKNGREASQLAYLPTSAVAQSARDMAIK
                     NAYLMKKNGGRGPVLLTATPVVNTPIDAYNMLSHVLPKEYWQKMGIYGPDDFVKFFGK
                     TRLETVQKISGEVEEKMALVGFENLDALRGIFHRWTTLKTAEDVKDTVEIPELDEHQQ
                     DAPLTEEQLAAYEELRQQAEAAAKANNGVTTSVNEDGVIEHEKARPIFSIIRDMDRVC
                     TDMDLYYRRITYRFLPEYADAVQQLADSLPKQATSEDDDSDDSITQQSQYSLIDKGEF
                     IQLQVPEAFEQEVNKRLAKFGIDEQTVTHPVTPKYVKLIATLKEFFPEGKQIIFTDEK
                     TQHQKLKRIICNALNLEPSKVGILNAQTVAEAGKTGKKLKAVKPPKELPDEPTDAQIA
                     KYNEQMALYDAYIAQQNEMSLGGLEKIAADFQEGRTPIIICNKKAEVGINLHRGTTDI
                     HHLTLPWTPASIAQRNGRGARVGSNRASVRVHYYCGKGSFDEYRLKTLKRKAGWISDI
                     LRSDKSEMENADANDMIEMQMYTAKDDGERLAMMQVQMDKAKAAQRARQKEQATIDLQ
                     NYIKAQHAAGEDVEVLTAELERSKAELEKTTAEVAKFKQAVMAKAADNADWKARWGSV
                     HHTDRTLLAQYRASLKSAIQRKANISQAISRYEKLLNRTQKAATDIKRLRPLVEDAIN
                     KGILDVDPDLVNHASEFLVIGDRSWRVGQYYDCAGDIVRIKSLDFDSQRADVEIIFTF
                     KGTKSGNWDVKTLDKQVDVTPDEDAVMQKISGGVSIAGINDIISCDDFYRFQQRGMIK
                     ITDSYGVQTTESGYSIDFVGTYSDPLKHAVYPDRRDGALKSSIAKWVLGMMSEGNNRQ
                     IRSAETFLVELFGSNYGDVIASYGDTLSPEAIQEKIADAIARMPEKTSQGATRNGDSE
                     LEVTNAIFGTNEFRASDYEITTAQFGTIGIYSNKDEIKQSMDAASARIAAERKANLNH
                     AVSALTQSWVTAIREAATTGKITPAIADVVNDGSKFMDAYKMDAVQLPSAYGQLSYRM
                     TYNLVSMFSDLAILGLVDLNEVTPELLSMRKNHVEILQRINTVLAGRTDEEKQADADR
                     INLALGNITEEEIAARNEKQEELSSIQGDATSIAQSLGLNYRVSTADLKMMYAPKFAA
                     GEVFGLQEASGMKGVLFRAKDAIKAKFGARWLPAKAKNSDFPGNWWIIETKHNVADVL
                     AVIQQYA"
     gene            21192..21632
                     /locus_tag="RHV13_002545"
     CDS             21192..21632
                     /locus_tag="RHV13_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015974230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HDV1242070.1"
                     /translation="MATLSDTIKPNKTYLEAVLRTALLGKTEDEYVDFFLSGLRGRLL
                     KNPRLYRSYGPYWPEIKKLLLERGYGNFGRLVDRDVRKIYRYDRPALTLIAATLYSQE
                     RFDNGQIYSAWHLLPVPEEVDDQDYEFESYDLEVEALAQAGEKT"
     gene            21629..21877
                     /locus_tag="RHV13_002546"
     CDS             21629..21877
                     /locus_tag="RHV13_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="modulator protein"
                     /protein_id="HDV1242071.1"
                     /translation="MKKRYYTVKHGTLRALQEFADKHNVEVRREGGSKALRMYRPDGK
                     WRTVVDFKTNSVPQGVRDRAFEEWEQIIIDNALLLNAD"
BASE COUNT         5673 a         5206 c         5832 g         5328 t
ORIGIN      
        1 taggattgaa atgatgacgc cggaaacttc ttataaagcg tggaaacagc cacatcatag
       61 atgattgcaa cctgcttacg ggggatgccc ttctcgagca atcgccgcat ttgctgccat
      121 gtttcttctt ggtatttagg ccgacgtcca cctatacgac cttctgcgcg agctgcatca
      181 agtccagcgc gtgtacgttc aacgataagc tcacgttcca tttctgccag cgcccccatt
      241 acgtgaaaga aaaagcgccc cattggtgta ctggtgtcga tggagtcagt gagactccgg
      301 aagttaatgc ctctgtcacg cagctcttcc accagcacaa ctaagtgacg catgctgcgc
      361 ccaagacgat ctaacttcca tacgactagg gtgtcacctc tggaaagcat acggagaacc
      421 ttttttaacc cagggcgttc agcctttttg ccgctcgcct tgtcctcaaa aattagctca
      481 catcctgcgc tttcaagagc gtttcgttgt aaagcagtgt tttgttcatt tgttgatacg
      541 cgtacatagc ctattagcat attttctgct cactatcgtt atttatagca agctgcggat
      601 tttaattaac aaaaaccagt atgtgtggaa atcacaaagt acataccgtt tcccaatgat
      661 atttaattca ctattaagga aatagttatg tgtgatttca caataatgct cctctccatc
      721 cttggcgggg tgcattcgtt tctgaatggg gttcgtgaaa aacgttacga agcgtcatgc
      781 aggcaattga tggccgagtg tattgctgcc gtacttgcag gctttatagg catgtatttc
      841 gcggaatata agggtatgga tgaaagtctt cagaattgcg tgactattat ttgcagcatc
      901 aataacaggc tcattcttga aaagttacaa aggattatcg attcgcacct caatagaaat
      961 gcctcttaag caacaaatga ccggttgaga agttactttg cataccatta cctcctgaca
     1021 acgtaggagg gaacttgtgc ttgacacaca ggaattagct ccagttgcta ttgcgctcct
     1081 gctttcagta attggtggga taggcacgtt cctgatggat gtccgagacg gtcgccagtc
     1141 tggcaatttg ttgggattgg ttacggagat ctttgttgca gtgacagctg gcgcggtggc
     1201 gtacctattg gggcaacacg agggctggga gttatcaatt acgtacttaa tggtaacgat
     1261 agccagcaat aacggtcatg aggtgatttc agggatgaaa cgagtgaata tcgatagcat
     1321 tctgaatgtt cttacaagtt tggtgaaaaa gggaggcggg aaatgattgg ctggggtgta
     1381 tgcgttcttg cgttagcctt agccgatcgc tatttgctaa aacgcaagga catcacgcat
     1441 ttagaacttg gtgatgtgga aattaaaccg ggtttcatcc gggtgccgtt caaataccgg
     1501 tctaaattcc cgtttttgcg cggcgcaacg gtcagatatt ggatccgcga tgttcagaag
     1561 ccgacgacag tgattgaagg cgaacaacgt tgtctgacgt cggctgaaca gggcgaaaac
     1621 agtgaatggt tgtacatacc cactgaatat atgggtaaag gagagcgact gtggcatttc
     1681 aacgtcatgg ttacgcatgg cgactcgttc attaacccgt tgtatcggat tttccctgtt
     1741 actcagcaaa tccgcagaag ttacgtaata aatctcgcac aggatgtgtc agatgacgaa
     1801 aaataagtat gcaacggtcg attttgacca ggttaatgaa aaggggctga aatcccttat
     1861 cgcggcgatc aataaaaccg gggttacggt aattgaggtt gactccagca accgcgcaac
     1921 aacgaaagat ggcgttaaag ttaaaaccgc aaagctggtt cttaacgacg gacaaattct
     1981 tgccatacag gtaaacgata ctggcgatat atcgtctgtg aaactgaatg gaaaagctat
     2041 tcctaacgct cagtcgccgg atatcaagac gcttggtacc gtcatgggac aggcggctcg
     2101 caaaaactcc gcaaaattcc agaaatcact gatcgccaaa gcgaagcgtg ttgccaatcc
     2161 ggtagacaag aaaccggcag ttaaatccaa ctttcagcgc ctgcaagaag caaaacagcg
     2221 gaatgctcag gtggttgccg cttataaatc agctcagaac tcggtgtctt tcaatcaaca
     2281 gcagatcact gatttgcggg cgaagctgga taaggagaca ggccgactca ataacgaaaa
     2341 ggcccgaaat ggcgaactca aacgccgtct taagcaactg aaagcaggaa attaacatgg
     2401 aacagttcaa tatcaataaa ggggtgacgc tcaagcctgg gcttgacgtg cttcccccgc
     2461 cagtgactga tgatgaatat cgcgcattaa tggccggtga ggaccgctat ctgatgacgg
     2521 aatccaacac cctggaggaa atcgaggcta cgttcttcta tgacacgccg atccactggt
     2581 gtgctacgga tttactggag gcgattagtt ctactcgttt gcagttacac cggaccatgc
     2641 aggcatttgt ccgggcattg aaccagaagc tgaatggtac cggaatctct gcggggagtg
     2701 ataaaacggg ggatgtggcc cagagcggcg cgcgcgcgat cggcggcgct gaaattggcc
     2761 gggcacgtaa cgttaacggg ctgccggtct tgccagccat tattccgctc agtgatggtc
     2821 agactatcag cattctgttt catagcccga cagcggaaaa ccggatcacc aatagcgata
     2881 cgctggttgc tttccagttc ttactgaata aaaaagacgt tactcacacc gttgctccga
     2941 tgagtggacg tgatatgacg ctggcgcagg tcaccatgaa acttgccaac cttgcagaga
     3001 aaaactcggc aaaattccag cgtgcgcaga agaagaaaaa agcccttgtt gatgaaataa
     3061 cccaactaca ggctgacagt gaccagaaag aggatgccat gagcgacctc gcggatcagg
     3121 tggcagcggt agaagggcag aaggcagatc tggagcagaa aattaacgct gttgcatcgg
     3181 aagcggattc tctttatgaa gagaatgagc gtttgcagac ggagattgat cagctcaatc
     3241 gcactggtgg gcgcgatacc attgctccag cggggatgac tggtgggcac tctcgcgcgc
     3301 tgacggatcg ccttgccagt atcaaaaatc gtatgcatat ggacggggaa gcgacgctca
     3361 gtaatggtgc atcaatgaag caattcattg gggatggtga agggtatatc cagttaaccg
     3421 atccggatgg cagcgtatac atgatcaagg ctaaatccat acagggtgtg gacatggcag
     3481 atgcgatcgg caagctgttt aaagcctata aagcgggtaa tgtatcggaa tatctggtcc
     3541 aaccagaaga acataaaccg gaaaacgtcg aacctgaacc agcggaggat accggtagct
     3601 cttcgcctga accagaagtc tctgtaggtg catatcgata tgccctgcaa atgcgtccgg
     3661 cggcccctgg cgcaatacct gaaggtaaca aagcgattct gccgcgccct gatgaaggtg
     3721 acccgtatta tgaatatgca cgctacggca ttgctactta cgataccccg ctttctgatc
     3781 agcaaatgag tgagtacgac ctgaagttat tgcctcgcga ggattctttc gacttcctgg
     3841 cgaagacact tactaatggt ccgtttggca aatatgcaca aaaagctctg gagctggcca
     3901 ccagctcacc agacgagttc cgcgtaatgc tgaaaactca gtttcaaaaa actttcccca
     3961 atattgcgtt tcctgggggc gctggcaccg agaaaatggt gcagagcatg atcaatgcat
     4021 tgcaggccga agtcggtgag attactcagc cagaaccggc cccggcacag cctgatgaaa
     4081 cggttagcga agcagatgca gaggctaata aagccattga atatctcaat aacgtaatgg
     4141 atatgcaaag cactgacatg gcggagatcc gtaacgcccg gggcaatatc cgggaagcga
     4201 ttgtagccct tcaggctgcc ggacgttttg aggaaaacga agagctggtt aacggcgcag
     4261 ctcgccacct ggctgatctg ttggtagcaa tccagaaagc gggggtagcg gcatgacact
     4321 atcagctatt gagttaatgg atctcagcga taagttggat gctctgatgt ccaaagcggc
     4381 caccgcgagt ggcatggagt tgctggatat cagcgatgaa attgaccaga tcatgcaaca
     4441 gatggggtac ggcgcgtccg gcggtggtag tggcgatgaa aaacaacctt cggaacatga
     4501 tggtgtgcca aaactggttg ctgatttcct ggctgataaa ttcgtcgatc agagcacaga
     4561 tgcatttatc ggtaccttgc aggacttgag tcaatatgtt ggcacataca tcgacctgga
     4621 ccaggttaaa cagcacacgg cggcatggat agccgccaac attaaagagg cagcataagg
     4681 cgtaacaggg atgagcttaa gcgatcaggt ggtaatggcc accagcatag aaacgctgat
     4741 cgagctgcta aagaacctgc ccgattatgg gcgggtttcg tatgtggtga cagcgaaggg
     4801 agacgaggta aaaacagcgt ttgatatcgt cgatgcctca gctcttttgg tatccaatac
     4861 tctggatggg aaaattaatc ctgactatcc ccaggaactt cagccgcgcg accggacccg
     4921 cgcatccagc cttcttcagg ttaaccagat atccaaagat ttgcggcctg ctcagcttac
     4981 tgattccggt ttatccagcc atggcgcgcc gataattggt gaggacaatg ccgttgagtc
     5041 aggtaatgga cggaccatgg ggatcatcaa agcctatcag gacggtaatg cggatcggta
     5101 tcgggagtac ctgattgaac atgcgaccga attcggcata cggcctgaaa aggttgaatc
     5161 aatgacggct ccggtactgg tgcgccgccg gttaacgaag gttgaccgtg ttcagtttgc
     5221 caaggactca aatatttctg atctccagga aatggcagcc agtgaaaagg cttttgttga
     5281 tgccgacagc ataacaccgg cgatgatggc gttgtttaac ccgtcagaaa gcggagatct
     5341 gcttagccgc agtaatgacg cgtttattcg cggattcatg acgcaagttg gtgccacaca
     5401 ggcggctggc cttgtaactg aagatgggcg accaacacgg caacttgttg accgtataca
     5461 aaacgcgatc tttgccaagg catataagga tgcgcgcctg gtaaggatgg ttgcagaaga
     5521 acctgatccg gatatgcgta atgttctgac ggcgcttaat gcggcagcca atgattttgt
     5581 ccagatgcag gctttatcag gagaagcgca caagcaggct gtgacaacta ttgttgatgg
     5641 cattgagaca gcggatagcc tcgataaaaa ggcgctggcg gcattgaaag atgcggtaga
     5701 cctggtaagg caatcgaagg agtcaggcca gcatattacc gatgttattg ctcaggggga
     5761 tatgttcagc gaaacggccc cggaagtgaa agcactcgcg ttgttcatcg tcgcgaataa
     5821 ccgtagcgcg aagcgtatgg ccaccgcctt taagttgatg gcgcaacgta tcaatgatga
     5881 gttacagcac cagggccagg cgctcgggga tatgtttggc ggcggcgatg tgtcgttaca
     5941 ggatatcctt cgccaggtgt ctcaggaact ggaatacgaa ggcatgcaag ggatatccgg
     6001 cggtcttttc gagtccgttt ccggcggtag ttacaacggt gttgctccgt ataccagttt
     6061 gctattacat cgggcatccg gcatcaaaga cattattcat ctgatcaggc tgctttcccg
     6121 cacagatccc caggatgaac agcttgtaca agtgcttgcg cattttgttc gaatgcctgt
     6181 tgccgacgtg aaaaaatggt gccgattatt cggtatcagc aattcgttac ttcgcggatt
     6241 gttaaatcac gcatcctccc ttgggcgcga tggctttgac gagatagcgc aggcgataaa
     6301 aaacggagat atgccaccag ctattgactg gttttccatt cgcccaacca gggtgaaagc
     6361 attccttagc gcggcgcatt cggcatcacc attggcagaa atggttcaga ggttgtcgct
     6421 catattcaca gaccataccg cgttgggtga tctgactctg gacgagatga aagaagcctc
     6481 cattcagtgg gccgatcaac aaaatgaggt taactcagac ttcttgccag cattcaggaa
     6541 ggccgttagt aaagctgatg atgcccgtgg aattctgaag gcatttaagg cattgcaaag
     6601 tcaggttaat aaacatgtcg gtgatatcga tggggtaacg gcggaaggca gggatatcct
     6661 taaagagcac ggcataacgc cagagtttat tgatgagatc aggactgata tgcagcgtga
     6721 ggtcgtatcg tccctgcaaa ttgtagccag ggcgttggcg gatgctaatc cgaagagtgc
     6781 ggccattgtt aaccgggtta ttggtgatat tgaagcatcg gagggcatgg gggcgctgaa
     6841 actcttcctt tcgcgagcgt ttaatcctaa cggcaatatt ctccctggca ttattggtga
     6901 ggctaaaaag tatgtcagtg aagaagaact tgagcagctt gaccaactac ttaagcgatt
     6961 ctcatacaac ccgcagacac gctggcaaat gaatcagcga agtatgggtt cggtccacga
     7021 gaaagtgtta tctgccatga acagtgcgat cgcaaactca tccgtatctg aagaaaaagc
     7081 tcttgagtgg gccgactctt ttatcacgga agaagtggaa gaagcccgcg ctggacagaa
     7141 tggtgggata gacctgcgca aggaacttgc tgatatttat cgcctgaccg gcggtaaaat
     7201 ttcgacctta tcaaaggtgg ttcaccacaa gggaagggca tatgcaaatc ttaatggtgt
     7261 tgttgctgtc aatttgaacg atgaaaatgc aagtgcactg tggcacgagc tgggtcatca
     7321 tcttgagtac agtaaccctg gtttgttaga gaaagcccgg tcattcctga aggccaatgt
     7381 tgaaggggat aagccatctt tcgttaatat cggtgggcgt ggcaagcctg aatggtgctt
     7441 cagatctcga ttgagtaata tttatatggc gaaggtatac ccgccagtct cagtgagtaa
     7501 ctccgggaaa attcggcaga aatcaccgac catttccaaa acgtcagcaa cggaagtatt
     7561 ctctatggct cttcagttgt atcatgacaa agaggccgct gccgcatcac tgatgaatgg
     7621 tgacggattg ctggaactgt tattaggtgt ggcaaaggag ctaaataatg cagattaaaa
     7681 tcgcagcgcc attaggcgga gatgccatta tcgaatttga tgataatgaa gaagtttccg
     7741 ggcgtttaag cattatctcc ggtgacatta ccgaggacat gatcgctgaa gccatagctg
     7801 gagcaaatcc caatagctat atgggattcg ttaacaccct tgatgctccc gcaagtgatg
     7861 ttctccgaac gctgtatctt tacgctggct ggtttgttga ttggccagca gtagatggtg
     7921 gcgatgagga cgacgacgat gattttggtg atcatataga ccagatcata tattaaagaa
     7981 atcccgccaa tcggcgggat ttttacctca cgagaagctc ttttctgatg tcagccaaca
     8041 gtgctcgtgc aaatctctcg gaggtttttc gacaaatgcc tcttcagcca catcctgcca
     8101 ggggatttgt ttagcccatt cagctatagc gttatggttt gccgagaata acggtattgt
     8161 ataggccttc agccagtcta atgtgctgga gttgtgtttt tgagcgtgat gtttcgcatg
     8221 gtgtttggcg atcacgatcg taggcactac ccatttacta ccgtcagtca ttgtgaagtg
     8281 catattacgg ggaacagatg acttttccat caactcccga atcccgggaa atttccccaa
     8341 gatcaatcgg cgatattcat cgctattgcg cccaccaaac tctttggctt tgaattcaag
     8401 atatgcttca gaaacgagag gcgaatcctc tgtgtttaga gttatcgccg tgaaaaaacc
     8461 agcaggattg tttttactat gggccaaccg gtgatgcgaa tcataaaagt accctttctc
     8521 gcgttccgat ggtttcgaca gcaacagcag gcgcgagtca taattggtta aattgccagt
     8581 tatcactgca tgagcgcgat cgctgatttc cgccgagtta ataacgatga aaagatcgtg
     8641 cggtccagta aaaccagcca gagacacttc gttattcaga caataggtta tatagacgca
     8701 tccccatgtt tcactgatat gcaccaaccc tttgtcaggg tgcattctga aatgattgcc
     8761 aagaaaaggg tgtttttggg taactcgctc ccaataacgg aacatatagt caattattgt
     8821 ttttcgacta tcgttaatag caggagaaac aaccactgta cgggaacacg aatacagtat
     8881 tgtttgcagg atgctaatca ctgccacaat agaggtttcc ccaataccat gtggtgtggt
     8941 ggcagtgact ttggctccgg tgttctttat cgcgttaata atttttgctt gatgaggtgt
     9001 taactcaata tcaagcaact ctttcgctgc cagttcccaa ttgtctttat actgttctat
     9061 cagtgctaac caggcagatt cattctgtat acgattaatc actttccacc tcttccgtgg
     9121 tgttttctcg cagttctgtt aatgcagcac gacacaggtt ccgggctttg gctatagcca
     9181 cactttttac ctcatccgtc attgtgcagg taatgtactg atcgagttct tcagctcgga
     9241 tgatgctttt ccctatcata aactctattt gccagagcag atcggcatcc atcgccagta
     9301 tttcagcctg gccttctggg cctgcgggga agcaaacata agactgtttg cccaggccga
     9361 taactcgaca acttgcttca agaattgcac gcttgaggtc tgactttgta actgaaacag
     9421 gttgattttc acctgtgatt acgccgttga catggacgtg gaagggcatg tagcttgaaa
     9481 ttcgctctac tttccatacg ccagcaagcg atccttcatg cagcacgatg ggggtaaccg
     9541 cgagtttcat ctcaccatat aactgctggc agataactgg attactgaat acatccagag
     9601 gttcacactc aaacagcggc gcaatctgca tgaggtccat catggtcata ccaggggtac
     9661 gagcagtaat gaatctgcgc attccagtat ccattgtgcg ccagatagct acaccatgct
     9721 ttttgctcac ttcttcagta aagccaaggt ggcacatgat ggttttttcg atagccagat
     9781 cagagataga aaccttttcg cctggcactc catcgttatt gatggtcact tcgacactct
     9841 ggccattacg taggcggtat tgaatggttt tagtattttg tgctgtcata gtcttttctc
     9901 tgcttaaaaa ctgatgtatt gcgccttcag gttggttagg aatgttttcc caccagcgaa
     9961 agcaatatct cggggtgttc tattcgttaa aagcgcgtgc cattgccaac tttggcgttt
    10021 ttgagcgagt tcgtgctttt gtcggcgtct ggaccaccgc ttttctttca gtcgtttttt
    10081 acacattcaa aacggaatat cgtcgtcaaa gtccattgga ggttcgttat tggcgttgct
    10141 ctgaggttta ccgccaccac tgtattgctg gtggttttga ggttggtttg attgccccca
    10201 gccatttgag gactgtgaat cgtcacggcg agcgccgatc atttgcatgg tgccgccctg
    10261 gctgacgata atttccgtcg tgtaacgttc tacaccggcg tcatctgtcc acttacgggt
    10321 tttaagtttc ccttcgatgt agacctgaga accttttcgt aaatattcac tcgcaatttc
    10381 agcaagtttt ccgaacaaaa ctactttatg ccattctgtt tgctctttct gttggcccgt
    10441 ttgcttgtcg cgccatgatt cattcgttgc gatgctgagt cttccgaccg ctccgccatt
    10501 tggtatatac ctgatctccg ggtcttgccc cagggtacca atcaggatga ctttgtttac
    10561 accgcgttgt gccacttttc ttacctaata aaataaatta attagagcaa taatgtatat
    10621 ctttgaaacg tggctaacaa gtgatttgca ttatcctgtg ccttctaaag ggatcgagtc
    10681 agtcggtatt ggctgtgaat gagtgtttgt cctggagcgt aaaaaattcg cttatgaggt
    10741 ctttatgaag ggaaaaacag ccgcaggagg cggtgcaatt tgcgctatcg cggtgatgat
    10801 taccatcgtg atgggtaatg gcaatgtgcg aaccaaccag gcggggcttg agctgattgg
    10861 taacgctgaa ggttgccgac gtgatccata catgtgcccg gcgggggtat ggactgacgg
    10921 gattggtaat acacacgggg taacgccggg tgtgcgaaaa accgaccagc aaatcgccgc
    10981 tgattgggaa aagaatatcc tgatcgctga acgctgtatt aatcagcact tccggggcaa
    11041 agacatgccc gataatgcct tcagtgcaat gacaagcgcg gcattcaata tgggatgcaa
    11101 tagcttacgg acctactaca gcaaagcgcg aggcatgcga gtagaaacgt ccatccacaa
    11161 gtgggcgcag aaaggggagt gggtgaatat gtgtaaccat ctccctgatt tcgtgaacag
    11221 taacggcgtg cccctgcgag gtttaaagat tcgccgtgaa aaagaacgcc agctttgcct
    11281 gacggggctg gtcaatgaat aaactccggc agctccgccg actttcgaca atgaagttat
    11341 cgctggcggc gatagttttc gactcgattt tcatggcggt atatgtgctc aatgagacgt
    11401 ggccactgga accgctattg tatgctgggc ttcggctgtg cctgacattt ttgagcatgg
    11461 ctgcaagatt gatgcagcag aaagaaaccg cttcagattg tccacgccgc gcggtgcgca
    11521 aatatatggc acgcaggcga aggcgataat agttaacgag aaccccggca tccgccgggg
    11581 ttatttttgg tggttattta aacggattga ttgaattatt aaacgtgatg atgcttgcct
    11641 cacgcggtgc ctggacgtta gccgcttgcg gaacctcctt aattttcttg gtgacaggca
    11701 ggttgcgtgc gccaactttg atcagagatt cgaaaagtgt ggcaacgatt tttgcatcac
    11761 caggttcttt gaggcggaat gcgtcttttt gggcggcgga gacgaagatc gggaggttat
    11821 ccagttcgtc ttgcattgct gccagcacat cgtcgcggat acccgctgtt ttctccagca
    11881 aagcgattcg cgcttcagca tctgcgatct tggccattgc ttcgaggtgg cggccctggc
    11941 tttcgagtag tgcggtttcc agttctgccg tacgctctgt cgcctccacc atcatttcca
    12001 gttcagccat tttgccgtaa tgggatataa ctgcctgcac tgactcgtcg gagtacccat
    12061 gcgccgccag ggactctgcc agtagagatt tagaatccgc gctttcaaac attccggcgc
    12121 tggcaggatg atccagactg atatagttcg gcgttgtcac ataatccaca ccatggaagc
    12181 tggtggttac agcgattttc ccggactcac gcccgccagt ggcccagctc cagccaccag
    12241 ctcggctttc gatcatcgcg gcgacaattt tacccggctc tgtgttaaga atttcctgtg
    12301 tatgggtaac gattccgttg tcgtcaacag atatagccac tgtgcggcac gctggaacat
    12361 tgtcgattac gaccgggcga ccttccacca tgatcacgct ggtttctggt acttccagtt
    12421 tgccagtcag ctgtcggcga ccgtgaccgt aatagccgaa aagctctcca aggcgtaaac
    12481 cttcctgagt ttccttgctt tcaagcatgg tcttgaccgc gcttaataca tactgtcgcc
    12541 cgttctggcg accttttcga gcattgctat agagacaaaa gcggtcagtg accgttttca
    12601 aaacatcagt cattatcgtt tccctcttta aagaccgatt caaggatttg cgccagttcc
    12661 tgtggcggtg ttttgatgat ggaatccatc aggtgatcgt cgtcctcgct tttcgctttc
    12721 agttcgttca ccagtgcttc agagattttt tcgtcaatct ccagcacatc gctaaacagg
    12781 taacgtttga atgcatctga attggcgagg acgctgttat tgctgacggc atcgaggatt
    12841 tgcgtaacga tagtggcgta gttcgcctgc gagtcgcggt tatcgttgtg ctcttgttgc
    12901 agagcggtat taacggagtg gaattcgatt ttgtacgggc gatcaccttc cgggtatacc
    12961 ttgccgtact tgaaagcaag atgaatatcg atagcccgca gaatgaactc atctacgccc
    13021 tgctggatcc atgaggcgcg catggcggcc tgaattgccg tgcgcaggaa tccaccttca
    13081 ccaagcccgc cggacatttg atctgcccac cccaggaggg tgtaatcgag gccaagtgct
    13141 gccgccagct ggcgcatata ggtgagaatg tcttcaatgc cgttgatgtc agcctggatg
    13201 gtctgagtat caatagtcat ctgtcccttg ccgtcgccca taataggcag caaggtattg
    13261 gtcaccgtag gcatgttatt cgcgccgcgt gcgcgctttt ccatcaggtc agctgctcgt
    13321 ttaagcgtct gagtaatggt gcgtgaataa tcggctgctt ttaccggatc cagactattc
    13381 atcgccagac cgatgattcg gtcaattttc gacgcattaa aacgcgttgc cttcagcgag
    13441 cggatcgccg aacgcaggtt catgtacggc tcgtaggcgt attcgagcaa gctggtcccg
    13501 taattctggg tttcaatcgg cgtgcgctct tccggattat ccagcaggct gtaagcctta
    13561 tggccagtgt gcacaggcat aaggtttgac ttaggccgcc agtaggggat tttcataggg
    13621 ataatggtcc acggatcggc gaaaaccatt ttccctgacg cgtccttcag ataatcgccg
    13681 ctaaatcccg ccaggttacc gctgacctcg aactctttga tgaagctcgg aagggtgtaa
    13741 taggagcact caaaagacgt gatccctatg ccttctttgg cgtatggcct gacataagcc
    13801 accccaaata cagacatgat aaatgcccac ccggcgacct ctttgttgat ggttcgcccg
    13861 atgtcgttca tcagctcgtc acacaacgcc tgcgcgtcgt catagtcact atcgtttccg
    13921 ttgtgtaccg gcacgataga gaaggtttgt ccggtcttct tatcgaaaga gagcgcgtgc
    13981 gtaatatgga tgttcagcgc ggtggcgatc gtgctgtaaa ccgccatctc ttcgagtagc
    14041 ggatagcgtt gcaagcgatc ttccggcagt tgaacttcat caaagataaa gcgacttccg
    14101 tccaccagcc catcgccagc catgccacta tcgcccggtt ggccgcctaa gaagccggac
    14161 agttgtaccg gtgcccctgc gcgagaaaac aaatacccac ttccgccgtg cacagccagc
    14221 gcggacagga ggatgttgtc ccgttctccg ttgtctttaa aaacccccgc cagcgccttc
    14281 ctgaccgagg atagcgtgat tttattgtct gccaagattg caccttaatt agaataattc
    14341 acatcgtgtt tgaacggaat ttaacactag tcacttgtta aggattacca atgaacaagc
    14401 tatctattgg ggtgtttcgc tgttcaagtg tcagcgaaat attgaaatac attagggcaa
    14461 taacatctca ccgagcgccg attaaatatg gcgtggaaaa ggtggaaggc aaaagctatg
    14521 accgactgcg ccgggaggcg aatcagaagg cgatagattt gcttaattcg ctggtggacg
    14581 gcgcgacact gacagatgaa cagcgccaga tcctggctgg gtacaccggt gaaggcggca
    14641 ttggcgggtc cgtctccgaa tattacacac caaagccgat cgctgaaggt gtctgggaga
    14701 tcatgaagct ctacggcgcg gacgtaggta acactctgga accatcggcg ggaaccggcg
    14761 tttttaatga gacaaaaccg gttggtacgg tgatgaccgc gactgagatc agcagtgttt
    14821 ccggtcgtat aaaccagttg ttacacccgg aagacagcgt acagatttcc ccgttcgaac
    14881 agctggctgt aagcacgcct aacgattcat tcgaccatgt tgtgggtaac gttccgttcg
    14941 gcggtcgtga taacacacgc aacatcgata agccttacgc agaagaaacg gacatgggtt
    15001 cttacttcat gctccgcatg ctggacaaaa taaagccagg cggattcatg tgcgtgattg
    15061 tgccgccgtc cattgtttca ggttcaaaca tgaagcggtt acgcctgcgc ctatcacgga
    15121 aagctgaatt tcttggtgcc caccgcctgc ctaccggtac ttttgacgca aacgggacca
    15181 gtacggtcgt agatgtggtg ctgatgcgca aacatccggc agagatggct gagaaaatcc
    15241 ccctggtgga tgaaagcact cttgaatcgg caaatgtgct ttggccaacg tttatttctg
    15301 gcaagtggtt tgaaaaggat ggccgccggt ttgttcatgg cacccaggaa aagggcttcc
    15361 aggggcgtat tgaggttcgt gccgacggtc agattgataa ccaggctctt aaagcgaagc
    15421 tgattcatcg tttcgaaagc cgtatcgact ggtctttgct cgatatggct gaaccgtcac
    15481 cgaccgcaga cgttgttggt gaaggggaaa tgcgcctgat taatggcgta tggcaaaaat
    15541 atgctggtgg tcgctggatt gaagctgatg ccgggaagga acttaagatc gatgctgcca
    15601 gttatggcgc ggatagctgg gaggctcttc agcgtaacct gactacaaca gaaggccgtc
    15661 tcggcatgac atttacccag atggcaaatg tccgcgataa gtacaccaca tcaatcagcg
    15721 acgatatggt gcagctggtg gactggatta acagccagcc tgaaaaatac cgtgaacgct
    15781 tgtatcgcgg ggcgatgatt ggccggatgt taattgaata tcaggacatg aaggccgccg
    15841 ggcatagtgc tgaacaaatc gaacagcagc gcctttctct ggtatcccgt ttgcaggcag
    15901 agattgaccg ttttggtaac cccggtcgcg gtccgatagc gaaattatcg gggagcggtg
    15961 cgcgcgcctg gtttgctttc cgtggtgcaa ttaagctgga tggcactatt tctgacgagc
    16021 tgacaggaaa gctggttacg catgattcca gcgcaagtta tgactccacc agctatcagg
    16081 acaccctgcg ttatctctac agtgatctca ctcgcgatcc aatccagctc gatgatttcc
    16141 gccttgcgtt taccggcgaa ctgccagcca gtgatgaaga gttgcttaat ttattggcca
    16201 gcacccctgg tattgcggtt tcaccgtatg gcgggattgt tccgttcgcc cgcgccacca
    16261 gcggcgacat taacgagata gtggctccaa aacaggaatt cctcgccacg ctccccgacg
    16321 gtccagtaaa gaacaacgtc cttaatcagc tggcagcgat cgaagagaag cgcatcaaga
    16381 cgccagcaga gaatatccgg tttaagctca atagccgttg gttcgaccgt tccgtcattc
    16441 tggaattttt gcaggaaaac ggctatccgg atctgcgcta tgtgcagtca gtgcagcttg
    16501 aaggcgacga aatggtttct gacacctatc acggtggtga tggtctgttc gtcgggcacc
    16561 gatacggtgt cgtccagcgc aaggataaag aaacaggcga gatccgctac gagtgggacc
    16621 gtaaatcagg tgaaaacgcg accgggttcc cggcacagct ggaaaagtat ctcaatggtg
    16681 cgcgtatcgg tggcaaagat agcgcgacgg cgaacggcta ccgcgagcag atggcactgc
    16741 ttgaggacca gttcaataag tggatcaaga cgcacgatcg ctacgatgag ctggttgcca
    16801 aatacaacga tgttttcaat agcaatatcc cgtatgaaca ctctggcgat ccgcttgggt
    16861 tgaagggatt aagcggtaag cgacagccat ttgattacca gaatagcgag gtgcgccgac
    16921 tgtccgaaga tgggcgcggc atcctgggct tcggtaccgg gctgggtaaa accacgaccg
    16981 cgctggcgct tgaggcgttc aactatgaga acggtcgttc cacccgtact gcgtatgtag
    17041 tgcctaaatc agtgctggaa aactggtatt acgaagcaaa agagtttctg agtgaagagg
    17101 cattcagtaa ctacctgttc gtcggtcttg atgtgctgat ggatggcgat cagattcgcc
    17161 aggtgccggt gctcgatgag aacggtaaac ctgtcgttgg tgcagatggc actcccgtta
    17221 tgcgcgatgc ccttaagctg gcagatgaag ccactatcac ggcgcggatg aacgcgatcc
    17281 cgcactcaaa ctaccgtgca gtcgtgttta ccaaagaaca atacgcccgc attccgctac
    17341 gtgatgacac cgtagatgag catgcacaag acatgcttta tgacttcgtt gctgccgggc
    17401 gtgtggccag cgcaatggac tccgattccc atcgcaaaga ggcggcgcgt cgccgggtat
    17461 tgtcggagta ttcagatacc ggtaccgaaa aagcagagaa gtatccgtac tttgaggata
    17521 tgggcttcga cagcgtgatt gctgacgaag gccacaacta ccgcaatagc tataaaaatg
    17581 gtcgcgaagc gtcacagctg gcctatctgc ccaccagcgc ggtggcgcaa tcggcgcgag
    17641 atatggcaat caaaaacgcg tacctgatga aaaagaatgg tgggcgtgga ccggttctcc
    17701 tgactgcaac gccagtcgtt aacaccccga tcgatgcata caacatgctt tctcatgttc
    17761 tgccgaagga atactggcag aagatgggga tctacggtcc tgatgacttc gttaaattct
    17821 tcggcaagac caggctggaa acggtacaga aaatcagcgg tgaagttgaa gaaaaaatgg
    17881 cgctggtggg ctttgaaaac cttgatgcgc tgcgcggcat attccatcgc tggacaacgc
    17941 ttaaaacggc ggaagacgtt aaggataccg tggagatccc ggaactggac gaacaccagc
    18001 aggatgcacc acttactgaa gaacaactgg cggcgtatga agaattgcgt caacaggcgg
    18061 aagcggcagc aaaagccaac aatggcgtaa cgacctcggt taatgaagac ggcgtgattg
    18121 agcacgagaa agcccgtccg atcttctcaa taatcaggga tatggaccgc gtatgtactg
    18181 acatggacct gtactatcgc cggatcacct atcgtttcct gccggagtac gccgatgcgg
    18241 tgcagcagct ggcggacagt ttgcctaaac aagccaccag cgaagacgac gacagtgatg
    18301 attcaatcac gcagcaatcg caatactccc tgatagataa gggcgagttt attcagttgc
    18361 aggttccgga agcgttcgag caggaagtga ataagcgcct ggccaagttt ggcattgacg
    18421 aacagaccgt aactcacccc gttacgccca aatacgtgaa gctgatcgcc acgctgaagg
    18481 agtttttccc ggaaggtaag caaatcatct tcacggacga aaaaacgcag caccagaagc
    18541 tcaagcgcat tatctgcaat gctcttaacc ttgaaccttc aaaggtgggg atcctgaatg
    18601 ctcagacggt tgccgaggca ggtaaaaccg gtaagaaact gaaagcggtt aaaccgccga
    18661 aagagttacc ggatgaacca acagatgcac agatagcgaa atacaacgag caaatggctc
    18721 tgtatgacgc ctatatcgcg cagcagaatg aaatgtcgtt gggcggtctg gaaaagattg
    18781 cagccgactt ccaggagggc cggactccga tcatcatctg caacaaaaag gcagaggtgg
    18841 gtatcaacct gcatcgagga acgactgaca tccatcatct gacgttgcca tggacaccag
    18901 ccagtatcgc acagcgtaac ggtcgcggtg cccgagttgg ctccaaccgt gcaagcgttc
    18961 gcgttcacta ctactgcggc aagggttctt tcgatgaata ccgactgaag acgctgaagc
    19021 gtaaagcagg ctggatctcc gatatcctcc gttcagataa gtcagaaatg gagaacgccg
    19081 acgccaacga tatgatcgaa atgcagatgt ataccgctaa ggatgatggc gaacgtctgg
    19141 caatgatgca ggttcaaatg gataaggcga aagccgcgca acgcgctcgc cagaaagaac
    19201 aggctactat cgaccttcag aactacatca aggcgcagca cgcagctggc gaggatgtgg
    19261 aggtacttac cgctgaatta gagcgaagca aagcggaact tgaaaagacc accgccgagg
    19321 tagctaaatt caaacaggcg gtaatggcca aagcagctga taacgcagac tggaaagccc
    19381 gctggggtag cgtccatcac acagaccgta cgttgttagc acagtatcgc gcgtcgttga
    19441 aaagcgccat tcagcgcaag gctaatatct ctcaagccat ctcccgctat gagaaattat
    19501 tgaaccgtac tcagaaggcc gcgacggata tcaaacgcct gcgcccgctg gtggaggatg
    19561 caataaataa aggcattctg gatgttgatc ctgacctggt taaccatgcg agtgagttcc
    19621 ttgttatcgg cgatcgctca tggcgtgtag gccaatacta cgattgtgcc ggtgatatcg
    19681 ttcgcattaa gtcgctggac ttcgacagcc agcgcgcaga cgtggagatt atctttacct
    19741 tcaaaggtac caaatcgggt aactgggatg tgaagacgct ggataaacag gttgatgtaa
    19801 ctcccgatga agatgctgtt atgcagaaaa tcagtggtgg cgtctccatc gccgggatta
    19861 acgacatcat ttcctgtgac gatttctacc gtttccagca gcgcggcatg atcaaaatca
    19921 ctgactcata cggcgttcag actacagagt caggctatag catcgatttt gttggtacct
    19981 attcggaccc actgaagcat gcggtttacc cggatcgccg tgacggcgcg ctgaagtcgt
    20041 caattgcaaa atgggtgctt ggtatgatgt cggaagggaa taaccgccag atccgttcgg
    20101 cagaaacatt cctggttgaa ctgtttggct ccaattatgg cgatgtaatc gcgtcatacg
    20161 gagatacgct atcccctgaa gcaattcagg agaaaatagc ggatgcgatc gccagaatgc
    20221 cggaaaaaac aagccagggg gctactcgta acggggattc tgaacttgaa gtcaccaatg
    20281 ccattttcgg taccaatgag ttccgggcgt cagattatga gatcaccaca gcacagtttg
    20341 gcaccattgg catttacagc aataaagacg agatcaaaca gtcaatggac gctgcaagcg
    20401 cgcgcattgc agcagaacgc aaagccaatc tgaatcatgc agtttccgcg ctgactcaat
    20461 cgtgggtaac agcaatcagg gaggccgcca ccacaggaaa aatcacaccg gcaattgcgg
    20521 atgtcgtaaa cgacggctct aaatttatgg atgcctataa aatggatgcg gtgcagttgc
    20581 catcagccta tggtcaactc agctatcgca tgacctacaa cctggtatca atgttttccg
    20641 accttgccat ccttgggctg gtggatctta acgaggttac gccggaattg ctcagcatgc
    20701 gcaagaatca tgtggagata ttgcagagaa ttaacacggt tcttgccggg cgcaccgatg
    20761 aagagaaaca ggccgacgct gatcggataa acctggccct tggcaacatc acggaggaag
    20821 agattgccgc cagaaacgag aaacaagaag agttatcatc aatacagggt gatgccacca
    20881 gcatagctca gtctcttggt ctgaattatc gcgtatccac cgccgacctg aagatgatgt
    20941 acgcaccaaa attcgccgct ggcgaggtat ttgggcttca ggaagcctca ggcatgaaag
    21001 gcgttctttt ccgtgcgaaa gacgcaatca aggcgaaatt cggcgctcgc tggctgccag
    21061 cgaaggcgaa gaacagcgat ttcccgggta actggtggat tatcgagaca aaacacaacg
    21121 tggcggacgt tctggccgtc atccaacaat acgcataaca ggagcgcccg gttcgccggg
    21181 cgtcgcataa tatggccaca ctatctgata caataaaacc gaataaaaca tatcttgagg
    21241 cggtactccg tacagcgttg ttaggaaaga cagaagacga atacgttgat ttcttcctgt
    21301 cagggctacg cgggcgatta ctgaaaaatc cccgcctgta ccgcagctat ggcccatact
    21361 ggccggaaat taaaaaatta ttactggagc gcggttatgg taatttcggt cgtctcgttg
    21421 accgtgacgt tcgcaaaatt taccgttatg accgcccggc gctgacgctc atagctgcga
    21481 cgctctacag ccaggagcgt tttgataatg gtcagatata ctcagcctgg catttactgc
    21541 cagtgcctga agaggttgac gaccaggact atgagtttga gtcttacgat ttggaagttg
    21601 aagccttggc acaggctgga gagaaaactt gaagaagcga tactacacag taaagcatgg
    21661 gacgctacga gcattgcaag agtttgctga taagcataac gttgaggtgc gcagggaagg
    21721 gggaagtaaa gctctgcgca tgtaccgtcc tgacggaaaa tggcggacgg tcgtcgattt
    21781 caaaactaac agtgttcccc agggcgtccg cgaccgggca ttcgaagaat gggagcagat
    21841 catcatagat aacgcattgc ttctgaacgc tgattaataa tcatttaaag cccatatgat
    21901 atgggcttaa acaacaggat cgatatacag tgttagttaa atatataggc gagagttata
    21961 tggaaaaacc ttggccgggc atgcacatta gaacatcttg tctggaggat ggtttcttta
    22021 gagcaacgca accaaagta
//
LOCUS       DAPGOF010000078        21593 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.078, whole genome shotgun sequence.
ACCESSION   DAPGOF010000078 DAPGOF010000000
VERSION     DAPGOF010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.078"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            131..673
                     /locus_tag="RHV13_002547"
     CDS             131..673
                     /locus_tag="RHV13_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242072.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            773..1975
                     /locus_tag="RHV13_002548"
     CDS             773..1975
                     /locus_tag="RHV13_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HDV1242073.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            2195..2977
                     /locus_tag="RHV13_002549"
     CDS             2195..2977
                     /locus_tag="RHV13_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDV1242074.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCNAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTKLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPATVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            2992..4197
                     /gene="rhmD"
                     /locus_tag="RHV13_002550"
     CDS             2992..4197
                     /gene="rhmD"
                     /locus_tag="RHV13_002550"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HDV1242075.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLVVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LILDCWMSQDVTYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            4254..5543
                     /locus_tag="RHV13_002551"
     CDS             4254..5543
                     /locus_tag="RHV13_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1242076.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFINLFGAVGGFIAPILRVKAETLFS
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            5561..6364
                     /gene="yfaU"
                     /locus_tag="RHV13_002552"
     CDS             5561..6364
                     /gene="yfaU"
                     /locus_tag="RHV13_002552"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HDV1242077.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVVSATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(6405..7403)
                     /locus_tag="RHV13_002553"
     CDS             complement(6405..7403)
                     /locus_tag="RHV13_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HDV1242078.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQSLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGHQIGWQEGKLVGLQ
                     EGKLEGWQEGKLEGWQEGMHEQAIKIALRMLEQGFDRDLVLAATQLSEADLAANNH"
     gene            complement(7596..8786)
                     /gene="glpC"
                     /locus_tag="RHV13_002554"
     CDS             complement(7596..8786)
                     /gene="glpC"
                     /locus_tag="RHV13_002554"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HDV1242079.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSVAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(8783..10042)
                     /gene="glpB"
                     /locus_tag="RHV13_002555"
     CDS             complement(8783..10042)
                     /gene="glpB"
                     /locus_tag="RHV13_002555"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HDV1242080.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQAVTDIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELGLAVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKEDFFAPQPWQQFGVTTDKTLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(10032..11660)
                     /gene="glpA"
                     /locus_tag="RHV13_002556"
     CDS             complement(10032..11660)
                     /gene="glpA"
                     /locus_tag="RHV13_002556"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HDV1242081.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAQTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            11933..13291
                     /gene="glpT"
                     /locus_tag="RHV13_002557"
     CDS             11933..13291
                     /gene="glpT"
                     /locus_tag="RHV13_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HDV1242082.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHQELLQKRN
                     GG"
     gene            13296..14375
                     /gene="glpQ"
                     /locus_tag="RHV13_002558"
     CDS             13296..14375
                     /gene="glpQ"
                     /locus_tag="RHV13_002558"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDV1242083.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLHKDN"
     gene            complement(14420..15298)
                     /locus_tag="RHV13_002559"
     CDS             complement(14420..15298)
                     /locus_tag="RHV13_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV1242084.1"
                     /translation="MTLTQIHTLLAVLDYGGFTEASKRLFMTQSAVSQAIATLEQELG
                     VEILIRERRKALQLTDAGSRIASHLRAITREVNAVKEIAEQEKHNPTRTLRIGCFPSA
                     CACILPGIIRYFETYHPQIKIIPYEENSSEIIDSVQNETIDAGFVHFPVTGMYSVPVY
                     QDKFTVVLPDAHPLAKNNAISVEELVGEPLIISKGRYELSIMALFKEKNITPVIKYEF
                     NHPDTAISFIRQGLGIALLPELTLKAVAGELCSVPLEPTFYRQISLLVKEAPVEGSPL
                     FLLQMCMETLVVKGIL"
     gene            15463..16653
                     /locus_tag="RHV13_002560"
     CDS             15463..16653
                     /locus_tag="RHV13_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1242085.1"
                     /translation="MKEKLWTKDFWAITIISFIIFFVFYVLLTLLPIYISDRLHASPD
                     KAGLLVTLFLIAAIVIRPFAGQWVGKYSNKTILVLSSLAFLVVTALYPFCHSIESLLF
                     IRVLHGITFGVITTVKGTISARLIPASRRGEGISFFSLAMGLAMVVGPWIGLNMARWE
                     AFNLAFWLCTGVAAVGIILSLIMTVPPVISHADGSTPKMGFAAMFDRAALPFAMVTFF
                     MTFSYAGVSAFLALYARELNLMSAASNFLLCYAIFLMICRTFTGNVCDKKGPKYVVYP
                     CLVFFTVGLVVLGYTQGSIMMVVSGALIGIGYGSVTPVFQTQIISSVEPHKIGVANSL
                     FFNAMDAGLALGACVMGMMVAHTGYRMIYLLGALLVVVAGGVYALQMKGKSGVALVVA
                     KEIH"
     gene            16734..17384
                     /gene="inaA"
                     /locus_tag="RHV13_002561"
     CDS             16734..17384
                     /gene="inaA"
                     /locus_tag="RHV13_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HDV1242086.1"
                     /translation="MAVSAKHDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHSVTPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(17438..17692)
                     /gene="yfaE"
                     /locus_tag="RHV13_002562"
     CDS             complement(17438..17692)
                     /gene="yfaE"
                     /locus_tag="RHV13_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HDV1242087.1"
                     /translation="MACVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(17692..18822)
                     /gene="nrdB"
                     /locus_tag="RHV13_002563"
     CDS             complement(17692..18822)
                     /gene="nrdB"
                     /locus_tag="RHV13_002563"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HDV1242088.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(19055..21340)
                     /gene="nrdA"
                     /locus_tag="RHV13_002564"
     CDS             complement(19055..21340)
                     /gene="nrdA"
                     /locus_tag="RHV13_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HDV1242089.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
BASE COUNT         4945 a         5329 c         5858 g         5461 t
ORIGIN      
        1 aattatttaa gaagtattta gttacaataa gtgactagat cccttttttc gctcattgaa
       61 tcctctgcgc caggcagtac aatcccgcag ctttgggaat tttgattaat tgcttgtggg
      121 aaaggggata atgaaaaaaa ttgcgctagc aggtctggcc ggaatgctgt tggtttctgc
      181 atcggtcaat gcaatgagca tcagcggcca ggcgggtaaa gaatacacca atattggtgt
      241 cggttttggg actgaatcga cgggcctggc gttaagcggt aactggacac ataacgacga
      301 cgacggtgac gtcgcgggcg tggggctggg gttgaatctg cctctcgggc cgttaatggc
      361 gaccgttggc ggaaaaggcg tttacaccaa cccgaattac ggcgatgaag gttatgccgc
      421 agcggtagga ggtggtttgc agtggaaaat tggcaacagc ttccgtttgt ttggcgagta
      481 ttactactct ccggattcac tctccagcgg tattcaaagt tatgaggaag cgaatgctgg
      541 cgcgcgttac accattatgc gtccagtcag tattgaggcg ggttatcgct acctgaatct
      601 gtcgggtaaa gacggcaacc gcgacaacgc cgtggctgac ggcccgtatg ttggggttaa
      661 cgccagtttc tgatcccttc cggcgcggca ttctgccgcg ccattcagtt tcttactacg
      721 ccttatctcc ctttgcgtta tagtgattcg ataacaaaag caggagataa ccatgttaaa
      781 agtggaaatg ctatccaccg gggatgaagt gttacacggg caaatcgttg acactaacgc
      841 tgcctggctg gccgattttt tctttcatca ggggttgcca ttatctcgcc gcaatacggt
      901 gggggataac cttgatgact tagtcaccat tcttcgcgaa cgtagtcagc acgccgatgt
      961 gctgatcgtt aacggcgggc tggggccgac cagtgatgat ttaagcgcac tcgccgctgc
     1021 gacagcaaaa ggtgaaggcc tggtgctgca tgaagcctgg ctcaaagaga tggaacgcta
     1081 tttccacgaa cgtggacgag taatggcacc gagcaaccgt aaacaagcgg agctgcctgc
     1141 cagtgctgaa tttatcaata acccggtagg caccgcctgt ggttttgccg tgcagcttaa
     1201 tcgttgcctg atgttcttta ctcccggcgt accgtcagaa tttaaggtga tggtcgagca
     1261 cgaaatcctg ccgcgtctgc gcgagcgttt ttctttacca cagccgccgg tttgcctgcg
     1321 tttgactacc tttggtcgtt cggaaagcga tctggcacaa agcctggaca ctctacaact
     1381 gccgccgggc gtaacaatgg gctatcgctc ctcaatgccg atcatcgaac tgaaactcac
     1441 cggaccggca agcgagcaac aggcgatgga aaaactgtgg ctggacgtta aacgtgttgc
     1501 cggacagagc gtgattttcg aaggcaccga aggactgccc gcgcagatca gtcgcgaatt
     1561 gcaaaaccgc cagttcagcc tgacgttgag cgagcaattc accggtggtt tattggcttt
     1621 gcaactttct cgcgcaggtg ctccactgct ggcgtgtgaa gtggttcctt cacaggaaga
     1681 aaccctggcg caaactgcgc actggattac agaacggcgg gccaaccatt ttgccgggct
     1741 ggcactggct gtttcgggtt tcgagaacga gcatctcaac tttgcgctag ccacgccaga
     1801 tggcactttc gctctgcgtg tgcgtttcag cactacacgc tacagcctgg ctatccgtca
     1861 ggaagtgtgc gcaatgatgg cactgaatat gctacgccgt tggttaaacg gccaggacat
     1921 tgccagtgag catggctgga ttgaggttgt tgagtccatg accttatccg tctgattccg
     1981 ccactctgtg ccggtttact gcaccggcac attccccctc cggcaaaatt tgtccaacct
     2041 gttgtactca ataagaaaaa atccgtgcgc tgattcacaa atttgtgcgt aatttctcca
     2101 gtccgttgga cgaggagcgg gatttgatgc gcttcacaat tatactcccc ctgagcaacg
     2161 agactgattc cagtatctaa gactggagtc cagtatgttg gaatcaagca aagttcccgc
     2221 cctgacacgg gctattgata ttctcaattt gattgcccgc attggcccct gtaatgctgc
     2281 gacgatcatt gacacattgg ggatccctaa aagcacggcc tatttgctgc ttaatgaact
     2341 caggcgtcag cgttttctta gccttgatca ccaggaaaat ttttgtttgt ggaccaagct
     2401 ggtggagctt tccggtcatg cgttaagcaa gatggatctc cgggaactgg ctcgaccgcg
     2461 tttgacgcaa ctgatggata ccaccgggct gttatgtcac ctggggatta ttgataacgg
     2521 cagcgcttat tacattttga aagtggagtc atcagccact atcagcgtgc gttcacatga
     2581 aggaaaaagc ctttcgcttt atcgctccgg aataggtaaa tgcctgcttg cctggcaacc
     2641 tgcaactgta cagcaaagta ttatcgaagg gctggtctgg gagcaggcga cacccacaac
     2701 gattactcat ccacagcaac ttcacgaaga actggcgcgt attcgccgtc agggctggag
     2761 ctatgacaac ggcgaagatt acgccgatgt ccgttgtgtc gctgcgccgg tgtttaacgc
     2821 caataacgag cttaccgctg cgatctcggt ggtcggtacc cgcttacaaa tcaatgaaga
     2881 ataccgtgat tatctggcgg gcaaggccat tgcctgcgcc agggatattt cacgtctgtt
     2941 gggatggaaa agtccctttg acttacaagc ctcataacaa tggagaacat catgacccta
     3001 ccaaaaatta aacaggttcg cgcctggttt actggcggtg cgacagcaga aaaaggcgct
     3061 ggcggcggtg attatcacga ccagggggcg aatcactgga tagacgatca tattgccacc
     3121 ccgatgagta aataccgcga ttacgagcag tcacgccagt catttggcat taacgttctt
     3181 ggcacgttgg ttgttgaagt cgaagcagaa aacggccaga ccggattcgc tgtttcgaca
     3241 gccggtgaaa tgggctgttt tatcgtcgaa aaacacctta accgtttcat tgagggtaaa
     3301 tgtgtcagtg atatcaaact gatccacgat caaatgctca atgccaccct gtattactcc
     3361 ggctctggtg gtctggtgat gaatacgatt tcctgtgtcg atctggctct gtgggatctg
     3421 ttcggcaaag tggtcgggct tccggtttat aaacttttag gcggcgctgt tcgtgatgag
     3481 attcagttct acgccacagg tgcgcgtccg gatctggcaa aagagatggg ctttatcggt
     3541 ggcaaaatgc cgacgcactg ggggccacat gatggcgatg cggggatccg caaagatgcc
     3601 gctatggtcg cggatatgcg tgaaaaatgc ggtgaggatt tctggttaat actcgactgc
     3661 tggatgagtc aggacgtgac ctatgctacc aaactggccc acgcttgcgc gccctataac
     3721 ctgaaatgga tcgaagagtg cctgccgcca cagcagtatg aaggttatcg cgaactgaaa
     3781 cgcaacgcgc cagtcggaat gatggtcacc agcggtgagc accacggcac actgcaatct
     3841 tttcgtacgc tttcagaaac cggtatcgac attatgcagc cggatgttgg ctggtgtggt
     3901 ggcttaacca cgctggtgga aattgccgca atcgccaaat ccagggggca actggtggtg
     3961 ccgcacggtt cgtctgttta ctcccaccat gcggtgatca ccttcaccaa tacgccattc
     4021 agcgaattcc tgatgaccag tccggattgt tcaacgatgc gtccgcagtt tgacccgatt
     4081 ctgctcaatg agccggttcc ggtgaatggt cgtattcata aatcagtgct tgataaaccc
     4141 ggttttggcg tcgaactcaa tcgtgactgc aatttgaaac gcccctacag ccactaatca
     4201 ctccgggcgt tgctgtgaca acggtaacgc ccacatattc taaggagctt tttatgagca
     4261 ccgctttgct tgacgccgtg gtgaagaaaa accgtgtgcg tttaattccg tttatgttgg
     4321 cgctgtatgt gctggcgttt ctcgaccgtt cgaatatcgg ttttgccaaa cagacctacc
     4381 agattgatac cgggttgagt aatgaagctt atgcgctggg agcaggcatt ttctttgtgg
     4441 tatatgcgtt tctgggtgtt ccggcgaatc ttttgatgcg caaactgggg gccagaacct
     4501 ggattggtac gacaacactg ctgtggggat ttctttcggc agccatggca tgggccgata
     4561 ctgaagcgaa atttctgata gttcgcactc tgcttggtgc tgcggaggcc ggatttttcc
     4621 ctggtatgat ttatctcacc tcgcaatggt ttccgcagcg taatcgcgcc agcattatgg
     4681 ggctgttcta tatgggcgca ccgctggcat taacactggg atcaccgctt tccggcgcgc
     4741 tgctggagat gcatggattt atggggcatc ccggctggtt ctggatgttt gtgattgaag
     4801 gattgttggc agtcggcgct ggggtattca cattcttttg gcttgatgac acaccggagc
     4861 aggcacgttt tctgagtaaa caagaaaaaa ctttgcttat caatcaactg gcaagtgaag
     4921 aacaacagaa agtgacttct cggctgagcg atgcgctgcg taatgggcga gtctggcaac
     4981 tggcgattat ctacctgacc attcaggtgg cggtttacgg attaattttc ttcctgccga
     5041 cccaggttgc ggcattgctg ggaacaaaag tgggctttac agcgtcggtg gtcaccgcca
     5101 ttccgtgggt tgcggccttg tttgggacct ggcttattcc gcgctattcc gataaaaccg
     5161 gcgaacggcg taatgtcgca gcgctgacat tactggcggc gggcattggt attggtctgt
     5221 ccgggctgct ttctccagta atggcgatcg tagcgctgtg tgttgcagcc atcgggttta
     5281 ttgccgtgca gccggtgttc tggacgatgc cgacacaact gctttccggt acggcgctgg
     5341 ctgcggggat tggttttata aacctgtttg gtgcagtggg cgggtttatt gccccgatcc
     5401 tgcgcgtgaa agcagaaacg ttattttcca gcgatgcggc gggattactg acgctggcag
     5461 cggtggcggt catcggttcg ctgattattt tcactctgcg tgtaaatcgt actgttgcgc
     5521 agaccgacgt ggcacatcat taaataggtt aaggaacacg atgaacgcat tattaaccaa
     5581 tccctttaaa gaacgtttac gcaagggcga agtgcaaatt ggtctgtggt taagctcaac
     5641 aactgcctat atggcagaaa ttgccgccac ttccggttat gactggttgc tgattgatgg
     5701 ggagcacgcg ccaaacacca ttcaggatct ttatcaccag ctacaggcgg tagcgcccta
     5761 tgccagccaa cccgtgatcc gtccggtgga aggcagtaaa tcgctgatta aacaagtcct
     5821 ggatattggc gcgcaaactc tactgatccc gatggtcgat actgccgatc aggcacgtca
     5881 ggtggtgtct gccacgcgct atcctcccta cggtgagcgt ggagtcgggg ccagtgtggc
     5941 acgggctgcg cgctggggac gcattgagaa ttacatggcg caagttaacg attcactttg
     6001 tctgttggtg caggtggaaa gtaaaacggc actggataac ctggacgaaa tcctcgacgt
     6061 cgaagggatt gatggcgtgt ttattggacc tgcggatctt tctgcgtcgt tgggctaccc
     6121 ggataacgcc gggcacccgg aagtgcagcg aattattgaa accagcattc ggcggatccg
     6181 tgctgcgggt aaagcggctg gttttctggc tgtggctcct gatatggcgc agcaatgcct
     6241 ggcgtgggga gcgaactttg tcgctgttgg cgttgacacg atgctctaca gcgatgccct
     6301 ggatcaacga ctggcgatgt ttaaatcagg caaaaatggg ccacgcataa aaggtagtta
     6361 ttgatatcaa aggcccatgg ggatcggcta tgggcctgtg ttaattagtg gttattcgct
     6421 gccagatcgg cttcgcttag ctgggtggcc gcgagcacca ggtcacgatc aaagccctgt
     6481 tccagcatgc gcaaagcaat tttaatggct tgttcatgca taccttcctg ccaaccttct
     6541 aatttacctt cctgccaacc ttctaattta ccttcctgca aacctactaa tttaccttct
     6601 tgccagccaa tttgatgtcc ttcctgccag ccaatttgat gcccttcctg cctcagccgt
     6661 tcagcaatag tcattaatct ctccttttgt agtggtgaac gttcggcaat ctcctgaata
     6721 aaacgggtga aacgggaggt atcgccgcat tgcagcagat aattaaacag tgattgtagc
     6781 tggctgtcat tagtgaagcc tctaaccaaa agcgtggtga tcctgtcgac cataccgatt
     6841 aaatcgcggt cgcgaatatg cttttgaatc agttcaagca gagctatacg ccgatgttgc
     6901 atgatctcat cgtcaggtac gatggtaata tccaccaacg gaaaagcttc ggtgtacaac
     6961 tgccgggcaa gttgcggatc gtcaaactca tccagccagt tgagtgagta cgggtagggc
     7021 gaggtttcgc catgataaaa cagtaacggc accaccagcg ggactctgtc atagcctttg
     7081 tccaggtgac gctgcatggc ggcagtggca tagcgcatta gccgaaaagc catattcttt
     7141 tctgcagagc tttgatgttc aatcacgcag tagatatagc cgtcaccgtc gctggtttcc
     7201 acggaccaca aaacatccga gtagtaagcg cgtaaacttt tttcaataaa actagtgggt
     7261 tccaggcgta aggtttgcag gttgcaaagc ttgcgcagcg gttctggtaa atgtatttcg
     7321 agaaaatccc gtgcggtttc gggtgtgaac ataaaggttt taaatactgc atcatgcggg
     7381 gaggaggttg ttgattcggt catggttgtc cgtcacctga tatttatgat gacgcgacat
     7441 tacggttatc ccaggccttt gacatctgcc agtttcagcc tttgcgagac gttttcagag
     7501 gaattttcac tgcgttgcaa cgcatacatt gggcacggaa tcgtgtacgc aaaaatgccc
     7561 ggtaaccaca tgcttaccgg gcatcagaaa ggagtttaag ccagcgcctg ggccagcagc
     7621 gtaatcggat gttcgcagcg aaggcttgtg gacatctcaa tctgccattt acaggtttcg
     7681 cagtcggtga ccaccagatc tgcgccgctt tcttctatct ggcggaacag tggtgcgccg
     7741 atggcttgtg aggtggggta gttctctttt ttgaaaccgt aagtacccgc aataccgcag
     7801 cactgggaat ccagcaccgt taactcaagc cccgggatgt tacgcaacag ctccagggtg
     7861 tagagcgtcc agcccatttt ttccatatgg cacggagtgt gataaaccac tttcagcggc
     7921 agcggtttca gcggtaacgt tttgccttcg tccagcttgc gccacagcca gcgggtcgcc
     7981 agttcgatat gatcgcgcaa gcctttgttg tcgacattca gcacttccgg gtattcgtcg
     8041 cgcagggcaa atgtacaggt tgaggaggtg gcaatcaccg gaataccttt tactcccaca
     8101 gcttcgcgga tcgactctac attcgtaatt gcctgtttgc gtgctttatc ggtaaagccg
     8161 ttggcgatca gcggtacgcc gcagcatttt tctttgctga gcagttgtac accggtaccc
     8221 attgcgttga gcactttaat taaatcttta cctaactgcg gatggttgta gttaacgaag
     8281 cagccgtgaa agaaagcgac ctggtcttta tactgcgctt gctgagccgc cacgctgcga
     8341 taccagcgac gaaacgtgcc gaaggagtat ttcggtagcg tgcggcgatg atcgattttt
     8401 aacgccgcat caagcaactg ccgcaccggt ttcagcgagg tagcggtgtt gacgattggt
     8461 gcgaacggcg tggaaacgct acccatcagg tcggtatggc ttaaaacaaa attgcgcagc
     8521 gacgggcgcg tggtgtcata tttcgcccgt gcgcgctgga tgatatcgcc aatcttcaca
     8581 tcggacgggc aggcgacttc gcaacgtttg cagttgatgc aatatttcag tgcctcgtca
     8641 tacagtgcgc catctttcaa acgcagacgc tcgccatccg gcccggcttg ttttggccct
     8701 ggataaccgg gattcacccg gctcaccggg caggcggtgg tgcagacggt gcacttaatg
     8761 cagttttcga agctggtgtc attcattgtt ggcctcctgc gcgttgggca atctgttgag
     8821 cggcatgtaa agcactgacg gcacaaacac cgccgccgca tccctgggcg atgggatcaa
     8881 atccgcccag caccgaaccg atggcaaaca ggttctcaat ggtttgccct gcctgtgatg
     8941 ggcgtagtgt cttatcagtg gttacaccga actgctgcca cggttgcggc gcaaaaaaat
     9001 cttccttata ccattcaccc cgcgtggcgg tttgtagcac atcaaggccg agaatcggct
     9061 ctcgaatgcc gttacgttcc gctaccagtc cgccactaaa gaaactgccg ctggcgagaa
     9121 ccgcgaaacg tggacgtagc ggaatatcgg cgtgattgcg ggtccagatt tcgttcacta
     9181 cgccattttt acaggtcact tttttcactt catcgcccgg catccacacg ccaccctggc
     9241 gcacaaactg gcgctgtaac tggttttgca gacgaatgcc cagcacggaa ggcggcagcg
     9301 ttggcaaaag catcagtgaa caaggtagtt tttcattcaa ccaacgccac agtttgtcat
     9361 cagccagacc gaagcaggcg ggcatcagga tcatttcgca ggtattggcg acaggaataa
     9421 gcgcatcaag taacagcggc cagttttctt cattatcaag gaaacgggcg atattcaccg
     9481 cgcggaactc ggtggcgtta ttgcgcagga catccagttc cggcagctct atttctgcgg
     9541 tttcaacggc aaggccgagt tcacgcaacg aagctgccgc aaggtgcgcc tgaaaatcca
     9601 tcagtccgct aattcccact acacatattt tcttcgcggg cagcggccag acggggactt
     9661 ctggcgaact tagccaggta gagcgcagag tgccgagcgg cgtaatccgc tggtgagcca
     9721 gttctacgct gccctgcaat tgcgcaccgc tttcagcgat taatgcctgc gcctggcaag
     9781 cgagatcgag cacgcgttgt ggcccgagaa gagagtaagg atgtgcaggt gcctgctgac
     9841 gcaaagattc cagtccactg tgaatgtctg tcacagcttg accatctggc agatggctca
     9901 gcaaatccag cgaaccggat gagaaatgca gcgcgctttg accacgcgtg acaatggcac
     9961 agcgcaggcc gtgtttttgc agttgcaggc cacagagtaa tccggcgagg ccgccgccca
    10021 taatgacagt atcaaagcgc atctttctgc tccttctcca gaccacacaa tccctgataa
    10081 acccagcggg taaattcgct ttcgcgcagt gcatctcccc aggcgatggg ttgcacgcct
    10141 ttccagcgtt cgttaaggaa ggtggaaagt tgctcgatag attgcgcgga cgtcgtgacg
    10201 ttaaaacgtt gcagcagtcc ggcagcgcgg caggcgcaga gttcgccctg gcaggtaccc
    10261 atccccacac gggtacgacg gcgtaaatcc agcaggctat taacgtttaa attttctacc
    10321 gcgtactgca cttcacccgc agttaccgct tcgcactcac ataccaggct acggtgcaga
    10381 cggccttcgc tcagccaggc aggcgtacga tcgccatgac gataaaccgc agaaccgcgc
    10441 agcggggcag gcagggaaat gactttacgc aaggtaactt cagcgggttc ttgtgaacca
    10501 ggcagtgcca gatcggcagt cgtacagggg cgcgtgttgc ccagtttgcg gcataccgcg
    10561 tcggtagccc attcagccat cagccgatag gtcatcagtt tgccaccggt gatggtgata
    10621 aatccgtcca gaccatcgcg ttcagcatgg tcgagcagca cgatgccacg gctgacgtta
    10681 cgtccgctcg ggtcgtcatc gctggcaacc agcgggcgca cgccagaata ggcccgcaaa
    10741 atgcgcgttt gcgccatcac gggggccagt ttttcccctt cacgcagcag aatatcaacc
    10801 tcttctgccg tcactcgatt atcgtcaatc tcgttgtaat caatacgtaa agaggtggtg
    10861 ccaatcagcg aaatggtatc gccaggcacc agaatatcgg cgtcggaagg tttacggcag
    10921 cggttgatca catgctggtt aatgcggtga tccatgatca gcagcgatcc tttcgccggg
    10981 aacatgcgaa tgcgcaggtc ggcatattcg gcaatgtgtt gcccccagat cccagcggca
    11041 ttaaccacga caggtgcatg aagggcctga gtttcgccgg tgagatggtt acgtacacga
    11101 acaccgcaca ccgtcgcgcc ttcacgaatc agccccgtga cttcatgagc ggtaaggata
    11161 acggcaccgt gttctttggc atccagcatg tttgctgcgg tcagacgaaa tggatcaacg
    11221 gtgccatccg gaactttcac cgcgccaatc agtgccgggt taacggcagg ttcgataatg
    11281 cgcgcttgct gcgggtctat agcttctgcg ctgatccctg cttcttcgca ggcgcgaata
    11341 aaagtggcct ggaaggagag gtcatcttcc ggcagggtga taaacaggcc gttggttggt
    11401 tcaacgcagt gacgtgcaat gcgtttcagg atctggtttt cactaatgca ttcgcgggcc
    11461 gattccgcat cggttaccgc atagcgcgca ccgctgtgca gcaggccgtg gttacgcccg
    11521 gtggcaccgg ttgcgatgtc gtggcgctca accaaaatca cgcgcagccc gcgcagggca
    11581 cagtcgcggg caattccggc tcccgttgcg ccgccgccaa tgataatcac gtcacttgat
    11641 tgcgagtcgc gagttttcat tgttatccct ctgaagttcg ttttttacca tttagccata
    11701 gtaaaaacat gaattgtttg atttcgcgca tattcgctca taattcgaaa gtaaaacgtg
    11761 atttcatgcg tcattttgaa cattttgtaa atcttattta ataatgtgtg cggcaattca
    11821 catttaattt atgaatgttt tcttaacatc gcggcaactc aagaaacggc aggttctctc
    11881 actgaatcag gctgttaatc ataaataaga ccacgggcca cggaggcttt caatgttgag
    11941 tatttttaaa ccagcgccac acaaagcgcg cttacctgcc gcggagatcg atccgactta
    12001 tcgtcgattg cgctggcaaa ttttcctggg gatattcttt ggctatgctg cttactattt
    12061 ggttcgtaag aactttgcgc tcgctatgcc ttacctggtg gagcagggat tctcacgcgg
    12121 tgatttaggt tttgcgcttt ctgggatctc gatcgcctat ggattttcga aattcatcat
    12181 gggttcggta tcggatcgct cgaatccgcg cgttttcctg cccgcaggtt tgattctggc
    12241 ggcggcagtg atgttgttta tgggctttgt gccatgggcg acgtcgagca ttgcggtgat
    12301 gtttgtactg ttgttcctct gcggttggtt ccaggggatg gggtggccgc cgtgtggtcg
    12361 tactatggtg cactggtggt cgcagaaaga acgtggcggc attgtgtcag tgtggaactg
    12421 tgcgcacaac gtcggtggtg gtattccgcc gctgctgttc ctgctgggga tggcctggtt
    12481 caatgactgg cacgcagcgc tctatatgcc cgctttctgc gccattctgg tggcattatt
    12541 cgcctttgcg atgatgcgcg ataccccgca atcctgtggc ttgccgccga tcgaagagta
    12601 caaaaatgat tatccggacg actataacga aaaagcggaa caggagctga ccgcgaagca
    12661 aatcttcatg cagtacgtgc tgccgaacaa actgctgtgg tacatcgcca tcgccaacgt
    12721 gttcgtttat ctgctgcgtt acggcatcct cgactggtca ccgacttatc tgaaagaggt
    12781 taagcatttc gcgctggata aatcctcctg ggcctacttc ttctatgagt atgcgggtat
    12841 tccgggcacc ctgctgtgcg gctggatgtc ggataaagtc ttccgtggca accgtggggc
    12901 aaccggcgtt ttctttatga cactggtgac catcgcgact atcgtttact ggatgaaccc
    12961 ggcaggtaac ccaaccgtcg atatgatttg tatgattgtt atcggcttcc tgatctacgg
    13021 ccctgtgatg ctgatcggtc tgcatgcgct ggaactggca ccgaaaaaag cggcaggaac
    13081 ggcagcgggc tttaccggtc tgtttggtta cctgggcggt tcggtggcgg cgagcgcgat
    13141 tgttggctac accgtggact tcttcggctg ggatggcggc tttatggtaa tgattggcgg
    13201 cagcattctg gcggttatct tgttgattgt tgtgatgatt ggcgaaaaac gtcgccatca
    13261 ggaattactg caaaaacgca acggaggcta atggcatgaa attgacgctg aaaaacctta
    13321 gcatggcgat catgatgagc actatagtca tgggaagcag tgcaatggcg gcggacagca
    13381 acgaaaaaat agtcatcgcc catcgcggtg ccagtggata tttgccggag catacgctgc
    13441 cagcaaaagc gatggcgtat gcgcagggag cggattatct ggaacaggat ttggtgatga
    13501 ccaaagacga ccatctggtt gttctgcatg accattacct cgatcgtgtt actgatgttg
    13561 ccgatcgttt cccggatcgg gcgcgcaaag acggtcgtta ctacgcgata gatttcacgc
    13621 tggatgaaat taagtcgttg aaatttaccg aaggtttcga tattgaaaac ggtaaaaaag
    13681 tgcagactta tccagggcgt ttcccaatgg gtaagtccga cttccgggtg cacacctttg
    13741 aagaagagat tgaatttgtt caggggttaa atcactctac cgggaaaaat atcggtatct
    13801 atccagaaat caaagcgccg tggttccatc atcaggaagg gaaggatatt gcggcaaaaa
    13861 cgctggaagt gctgaagaaa tatggttaca ccggtaaaga cgataaagtt tatttgcaat
    13921 gttttgatgc tgatgagctg aagcgtatta agaatgagct ggaacccaaa atgggcatgg
    13981 atctcaatct ggtacagctg attgcctata ccgactggaa tgaaacgcag cagaaacagc
    14041 cggacggaag ctgggttaat tacaactacg actggatgtt taagccgggt gccatgaaac
    14101 aggtggcgga atatgcagat ggtattggtc cggattacca tatgttgatt gaggagacat
    14161 cgcagccggg taatatcaaa ctcactggca tggtgcaaga tgctcagcag aacaagctgg
    14221 tagtgcatcc ttataccgtg cggtcagata aactgcctga atacacaact gatgtgaatc
    14281 agttatatga tgctctgtat aacaaagcgg gtgtaaatgg gttgtttact gatttccctg
    14341 ataaagcggt taaattcctg cataaagata attaatgaca caggcatccg ggaaggcaaa
    14401 acctgaccgg atgccctttt cacaatatgc ctttgaccac cagggtttcc atacacattt
    14461 gcaataaaaa caatgggctg ccttctaccg gcgcctcttt gaccagcaat gaaatttgcc
    14521 gataaaacgt cggctccagc gggacagagc ataactcacc ggcgacggct ttcagcgtca
    14581 gctccggtaa cagagcaatt cctaatccct ggcgaataaa acttattgcc gtatctggat
    14641 ggttaaattc atatttaatg accggcgtaa tattcttttc cttaaataac gccataatgc
    14701 ttaattcata acgcccctta ctgataatga gcggttctcc caccagttct tccaccgata
    14761 tagcgttatt ttttgccagg ggatgagcat ctggcagcac cacggtaaat ttatcctgat
    14821 ataccgggac ggaatacatt ccggttaccg gaaaatggac aaaaccggca tcgatagttt
    14881 cgttttgcac cgaatcaata atttcactgc tgttctcttc gtaaggaatg atttttatct
    14941 gcggatgata agtttcaaaa tagcgaataa tacccggtaa aatacacgcg caggcgctgg
    15001 gaaagcagcc gatgcgcagc gttctggtcg ggttatgttt ttcctgctcg gcaatttctt
    15061 tcacagcgtt cacttcacgg gtaatcgccc gcagatggct tgcgatgcgt gagccggcgt
    15121 cagtcagctg cagtgctttt cgccgctcgc ggatcaggat ctctacgcca agctcttgct
    15181 ccagggtggc aatggcctgg cttacggcgg attgcgtcat aaacaggcgc ttactggctt
    15241 cggtaaagcc gccataatcc agtacggcga gcagggtgtg gatttgggtt agtgtcatta
    15301 gtaatatcta attaatatta ttaaaacgat taatttctct tatagagtgc atccatttat
    15361 actctatttc tatcgagtaa atcacatttt gacgcacctg tagtggttca gcctgtgatg
    15421 ctgagcggtg tccttgtgcg ccatttgaac gggtcgttta gtatgaaaga aaaattatgg
    15481 acgaaagatt tttgggcaat caccattatt agctttatta tctttttcgt cttttatgtg
    15541 ttgctgacct tattacctat ttatatctcc gatcgtctcc atgcatcccc tgataaagct
    15601 ggtctgctgg tgacattatt tttaattgcg gcgattgtta ttcgaccctt tgccgggcaa
    15661 tgggtgggta aatattcgaa taaaactatt ctggtgctct cttctctggc ctttttggtg
    15721 gtcactgcgc tgtatccttt ttgccactca atagaatcac tgctttttat tagggtgctt
    15781 catggtatta ccttcggggt tatcactacg gtaaaaggaa cgatttccgc gcggctgatc
    15841 ccggcctccc gacgtgggga gggcatcagt tttttctctt tggcaatggg gctggcaatg
    15901 gtggtcgggc cgtggattgg tctgaatatg gcgcgctggg aagcctttaa tctggctttc
    15961 tggctatgca ccggcgtggc agcggtgggg attattctgt cgctgattat gacagtgccg
    16021 ccggttatca gccatgctga tggttcaacg ccgaaaatgg gctttgccgc catgttcgat
    16081 cgcgcggcgt tgccatttgc gatggtcacc ttttttatga ccttttcgta tgccggggtt
    16141 tctgcttttc tggcgcttta tgctcgcgaa cttaacctga tgtcggcggc cagtaatttc
    16201 ctgctttgct acgccatctt cctgatgatc tgccgtacct ttaccggcaa tgtctgcgac
    16261 aaaaaaggac cgaaatatgt ggtttaccct tgccttgtgt tctttacggt aggactggtg
    16321 gttctcggct acacccaggg aagcataatg atggttgttt ctggcgcgtt gattggtatc
    16381 gggtatggtt ccgtgacgcc agtttttcag acgcagatta tcagttcagt ggaaccgcat
    16441 aaaatcggtg tcgcaaactc cctcttcttc aatgcgatgg atgcaggcct ggcgctggga
    16501 gcctgtgtga tggggatgat ggttgcacat actggctacc gaatgattta tctgctgggc
    16561 gcactattag tggtagtggc tggtggagtc tatgcgctgc aaatgaaggg taaaagcggt
    16621 gtcgcgctag tagtggcaaa agaaattcat taatacgaca tgtttcatta agatttttcg
    16681 caggtaacca cctatagtca ttgtcgttaa taaaaaatga caggcgatag ggtatggcag
    16741 tttcagcaaa gcacgacgaa tttaatcact ggtgggcaac agagggcgac tgggttgaag
    16801 agcctaacta tcgccgtaat ggtatgagcg gggtgcaatg cgtcgagcgc aacggcaaaa
    16861 agctatatgt aaagcgcatg acgcatcatc tgtttcattc cgtacgttat cctttcggcc
    16921 gaccaacgat tgtccgtgag gttgcggtaa ttaaagaact ggaacgggcc ggtgtcatcg
    16981 tgccgaagat cgtttttggt gaagcggtga aaattgaggg ggaatggcgc gcgttgctgg
    17041 tgactgaaga tatggcgggg ttcatcagca ttgctgactg gtatgcccag cattcagtaa
    17101 cgccctattc tgacgaagta cggcaagcca tgttgaaagc agtggcgctg gcgtttaaga
    17161 aaatgcatag cgttaatcgt cagcatggct gttgttatgt tcgccatatc tatgtgaaaa
    17221 cagaaggtaa ggcagaagct ggttttcttg atctggagaa aagtcgtcgc cgcttgcgtc
    17281 gcgataaagc gattaatcat gacttccgcc agctggaaaa atatctggag ccgatcccca
    17341 aagcggactg ggagcaggta aaagcgtatt actacgccat gtaatcgttg taggccagat
    17401 aatacgcgta agcgtcgtat ctggcgctag tacccgatca caactcgatt tcaatatcgc
    17461 cttttgcccg gcaacaacag ggcaaaattt cccccggctg aataaaggct aacggttcgg
    17521 caatccagtc cacctggcct gcaacaaggc gcgtgcgaca ggaaccgcag taaccttcgc
    17581 ggcactggta ctcaaccgcc acattgtggg attccagcgc cgccagaagg gaagggtgtt
    17641 catcctggca cagcagttgt gtgccagtga tgcgcagggt aacgcaggcc atcagagctg
    17701 gaagttactc aaatcgtcgg tgtccacttc cgcgtcaatc tgcccgacca gataagaact
    17761 cacctccact tcctgtggag caacctgcac gttatcagac accagccagg tgttgatcca
    17821 cgggatcggg ttggagcgcg tctggaacgg cagatccaaa ccaaccgcct gcatacggat
    17881 attggtgatg tactccacgt actggcagag aatgtctttg ttcagaccaa tcatcgaacc
    17941 gtcacggaac agataatccg cccagtcttt ctcctgttgt gccgcctgga caaacaggtc
    18001 atagcactcc tgcttacatt cttcggcaat ttccgccatc tccggatcat ccgcgccgct
    18061 gcgcagcaga ttcagcatat gctgggtgcc ggtcaggtgc agggcttcgt cgcgggcaat
    18121 caggcgaata attttggcgt taccttccat cagctcacgt tcagcgaatg cgaaagagca
    18181 agcaaagctg acgtagaaac gaatcgcttc cagcgcgtta acgctcatca ggcagagata
    18241 cagttttttc ttcaactcgc gcaggctaac ggttacggtt ttaccgttaa cggtgtgggt
    18301 gccttcgccc agcaggtgcc agtagctggt catttcgatc agctcatcgt aatagctgga
    18361 gatcccttcc gcacgtttct ggatctgctc gttggtgacg atatcgtcaa acacaacaga
    18421 cggatcgtta acgatattac gaatgatatg agtataggaa cgggaatgaa tcgtttctga
    18481 gaacgcccag gtttcgaccc aggtttccag ttccgggatc gagatcagcg gcagcagtgc
    18541 cacgtttggg ctacgaccct gaatggagtc cagcagcgtc tgatatttca ggttgctgat
    18601 aaagatgtgt ttttcgtgtt caggcagcgc ctggtaatcg atacggtcac gagaaacgtc
    18661 aacttcttcc ggacgccaga agaaagagag ctgcttttcg atcagctttt cgaagatgtc
    18721 atatttttgc tgatcgtagc gagccacgtt gaccggctga ccaaagaaca tcggttcttt
    18781 gagctgatca tttttcgtct gtgaaaaggt ggtatatgcc atgagtgtgt cctgttggga
    18841 gtttaatgcc ggataaggca ttttacgccg catcctgtgc ctgatgcgac gctggcgcgt
    18901 ctaatcaggc ctacaaaccg agccgtaggc cggataaggc gttcacgctg catccggcac
    18961 ccggagcctg atgcgacgct ggcgcgtctt atcaggccta caaaccgagc cgtaggcctg
    19021 ataaggcgtt tacgccgcat ccggcatctc aatatcagat cttacatgcg ccgctttcgc
    19081 agccatcgtc ctggattgac ggcaccagat cgtcctgcgc gtcttcagcg ccgtcacggg
    19141 tgttctgata atacagcgtt ttgaccccga atttgtaggc ggtgagcagg tctttcagca
    19201 attgctgcat cggcactttt cctgacggga agcgtgacgg atcgtagttg gtgttagcag
    19261 agatcgactg atcgataaat ttctgcatga tacccaccag ttgcaggtag ccatcgttac
    19321 ccggcatttc ccacagcagc tcataggcgt cgtgcagatg ctcgtagtcc ggcactacct
    19381 ggcgcagaat accgtctttc gacgctttga tgctgacgta accgcgcggc ggttcaatac
    19441 cgttagtggc gttagagatc tgtgaagaag tctcggacgg catcagggca gaaagcgtgg
    19501 agttacgcag accgtgcgtt ttgattgact cacgcagtgc ttcccagtcg taatgcagtg
    19561 gttcattagc gatggcatcc agatctttct tataggtatc gatcggcagg atccctttcg
    19621 cgtaagtggt ttcgttaaac cacgggcacg cgccttgctc tttcgccagc tcattagacg
    19681 ctttcagcag gtaatactga atcgcttcga aggttttatg cgtcaggttg ttggcgctgc
    19741 cgtcggagta acgtttaccg tgcttcgcca ggtagtaagc gaagttgatc acaccaatac
    19801 ccagcgtacg acgacccatc gctccacgtt tggcggccgg gatcgggtaa tcctgataat
    19861 ccagcagcgc gtcaagtgca cgaaccgcca gaatcgccag ctcttccagt tcatccaggc
    19921 tgttaattgc gcccagattg aatgcagaca gcgtacacag cgcgatttca ccgttttcgt
    19981 cgttgacgtc gttcagcggt ttggtcggca gggcgatttc caggcacagg ttagactggc
    20041 gcactggcgc gatggccgga tcaaacgggc tatgggtatt gcagtggtca acgttctgaa
    20101 tatagatacg accggtagac gcacgttcct gcatcatcag cgagaacagc tcaacggctt
    20161 tcacacgctg cttgcggatg ctgtcgtctt tctcatattt ggtatacagg cgttcaaact
    20221 cttcctgatc ggcgaagaat gcgtcgtaca gccccggtac gtcggacggg ctgaacaggg
    20281 tgatatcttc acctttcagc aggcgggtat acatcagttt gttgatctgt accccgtagt
    20341 ccatatgacg cacgcggttg ccttccaccc cacggttgtt tttcaacacc agcaggcttt
    20401 caacttccag atgccacatc gggtagaaca gcgttgccgc accgccgcgc acaccgccct
    20461 gagagcagga tttcaccgct gtctggaaat gtttgtagaa cggaatacaa ccggtatgga
    20521 acgcttcgcc accgcgaatc gggctaccca gcgcacgaat acgcccggcg ttgatgccga
    20581 tcccggcacg ctgggatacg tatttaacaa tcgcgctgga ggtggcgttg atggagtcca
    20641 ggctgtcgcc gcactcgatc agtacgcagg agctgaactg acgagtcggg gtacgcacgc
    20701 cggacatgat tggcgtcggc agcgaaattt taaatgtgga aaccgcgtcg taaaaacgct
    20761 tcacgtattg caggcgcgtt tcacgcgggt agttcgagaa caggcacgca gcaaccagaa
    20821 tataaaggaa ctgggcgctt tcgtagattt cgccggtcac acggttctgt accagatatt
    20881 tgccttccag ttgcttaacg gcagcataag agaaggtcat atcacggtcg tgatcgataa
    20941 acgtgtccat ctgcttgaac tcttcttccg tgtagtcttc cagcagatga ttatcgtatt
    21001 tgcccatctc gaccattttc accacatggt catacagcgc aggcggctca aactggccgt
    21061 aggctttttt acgcaggtgg aagatcgcca ggcgcgcggc gagatactga taatccggcg
    21121 catcacggga gatcaggtct gcggcagcct tgataatagt ttcgtggatg tcagaggtct
    21181 tgataccgtc ataaaactga atgtgggagc gcagctcgac ctgggaaatc gaaacgttat
    21241 gcagtccttc tgccgcccaa tccagaacgc gatggatttt gtcgagatta atgcgctctg
    21301 tgctaccgtc gcgctttgtc accagcagat tctgattcat gtatgtcgta cctgtttttg
    21361 gaattctttc cgcaatagtt tcatgcagac tacctgtagt acgggaagct gcttagaaca
    21421 ctatatatag ggggtatgtt tgggattcac tgcaagatag tgtgaaaatg gccctcttgc
    21481 aagtgcataa ctttgtggat aactcaggaa ggaaaaagtg gctttcgcgc accttaggtc
    21541 agacaaggtg tccgggaaag tcaatgggaa gagaaaaatt tgttaaaaat aac
//
LOCUS       DAPGOF010000079        21398 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.079, whole genome shotgun sequence.
ACCESSION   DAPGOF010000079 DAPGOF010000000
VERSION     DAPGOF010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.079"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..845
                     /locus_tag="RHV13_002565"
     CDS             <1..845
                     /locus_tag="RHV13_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HDV1242090.1"
                     /translation="VCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKELTHKLESIFNN
                     TQVQIAVLIVSTTKDETIEQYATRVFDNWRLGDAKRNDGILIVVAWSDRTVRIQVGYG
                     LEEKVTDALAGDIIRSNMIPAFKQQKLAKGLELAIIALNNQLTSQHQYPANPSESEST
                     SSTDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIHLLDLFLFSDKTFSI
                     AVFSFFFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSSGGGFSGGGGSSGGGGA
                     SGRW"
     gene            complement(905..2203)
                     /gene="eno"
                     /locus_tag="RHV13_002566"
     CDS             complement(905..2203)
                     /gene="eno"
                     /locus_tag="RHV13_002566"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDV1242091.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2291..3928)
                     /gene="pyrG"
                     /locus_tag="RHV13_002567"
     CDS             complement(2291..3928)
                     /gene="pyrG"
                     /locus_tag="RHV13_002567"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HDV1242092.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4156..4947)
                     /gene="mazG"
                     /locus_tag="RHV13_002568"
     CDS             complement(4156..4947)
                     /gene="mazG"
                     /locus_tag="RHV13_002568"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HDV1242093.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(5043..7277)
                     /locus_tag="RHV13_002569"
     CDS             complement(5043..7277)
                     /locus_tag="RHV13_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HDV1242094.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7325..8626)
                     /gene="rlmD"
                     /locus_tag="RHV13_002570"
     CDS             complement(7325..8626)
                     /gene="rlmD"
                     /locus_tag="RHV13_002570"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDV1242095.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVEPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8683..11439
                     /gene="barA"
                     /locus_tag="RHV13_002571"
     CDS             8683..11439
                     /gene="barA"
                     /locus_tag="RHV13_002571"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HDV1242096.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11672..13012)
                     /gene="gudD"
                     /locus_tag="RHV13_002572"
     CDS             complement(11672..13012)
                     /gene="gudD"
                     /locus_tag="RHV13_002572"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HDV1242097.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     IWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(13033..14373)
                     /locus_tag="RHV13_002573"
     CDS             complement(13033..14373)
                     /locus_tag="RHV13_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HDV1242098.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVLLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQPADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRDAITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRVLKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTQNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14375..15727)
                     /gene="gudP"
                     /locus_tag="RHV13_002574"
     CDS             complement(14375..15727)
                     /gene="gudP"
                     /locus_tag="RHV13_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HDV1242099.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16162..16611)
                     /locus_tag="RHV13_002575"
     CDS             complement(16162..16611)
                     /locus_tag="RHV13_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDV1242100.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNLWVEQWGTLLS"
     gene            complement(16629..17411)
                     /gene="truC"
                     /locus_tag="RHV13_002576"
     CDS             complement(16629..17411)
                     /gene="truC"
                     /locus_tag="RHV13_002576"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HDV1242101.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17411..17740)
                     /locus_tag="RHV13_002577"
     CDS             complement(17411..17740)
                     /locus_tag="RHV13_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HDV1242102.1"
                     /translation="MTTHDRVRLQLQTLEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18362..18907)
                     /gene="syd"
                     /locus_tag="RHV13_002578"
     CDS             complement(18362..18907)
                     /gene="syd"
                     /locus_tag="RHV13_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HDV1242103.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDVVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18975..19823
                     /gene="queF"
                     /locus_tag="RHV13_002579"
     CDS             18975..19823
                     /gene="queF"
                     /locus_tag="RHV13_002579"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HDV1242104.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERLFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19935..21299
                     /gene="ppnN"
                     /locus_tag="RHV13_002580"
                     /gene_synonym="ygdH"
     CDS             19935..21299
                     /gene="ppnN"
                     /locus_tag="RHV13_002580"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HDV1242105.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5241 a         5711 c         5118 g         5328 t
ORIGIN      
        1 tcgtatgttc ctttgttgca gcccaaccaa ctattgtccc acaattacag caacaagtta
       61 cggatcttac gagtagctta aactcacaag aaaagaaaga actgacgcac aagttagaat
      121 ctattttcaa taatacccaa gtgcaaattg ctgtattaat tgtttccaca accaaagatg
      181 agacaattga acaatatgct acaagagttt ttgacaattg gcgtttagga gatgccaaac
      241 gtaatgatgg gatactgatc gttgttgcct ggtcggatcg cactgtccgc atccaggtag
      301 gttatgggct ggaagaaaag gtaaccgatg ctctggctgg agatatcatc cgtagcaaca
      361 tgatacccgc atttaaacaa caaaaattag ctaagggatt agagttagct ataatcgctt
      421 tgaataacca actcacttca caacaccaat atccggctaa tccttcagaa agtgaatcaa
      481 cgtcttccac tgatcattat tactttgcta ttttttgggt atttgcagtg atgttcttcc
      541 ctttctggtt ttttcatcaa ggcagtaatt tttgtcgcgc atgtaaaagt ggagtctgta
      601 tttcagcaat ccatctttta gatttattcc tgttctccga taaaactttt tccattgctg
      661 tattttcctt cttttttact ttcaccatat ttatggtctt tacctgttta tgtgtacttc
      721 agaaaagagc atctggtaga agttatcatt cagacaacag cggttccgct ggaggctcaa
      781 gtggcggcgg atttagcggc gggggcggtt cttctggcgg tggcggcgca tccggccgct
      841 ggtaactcca ccataaaaaa tgccagcccg aaggctggca ttatcaaatc agataaagtc
      901 agacttatgc ctggcctttg atctctttac gaccgttgta cggtgctttt tcgcccagag
      961 cttcttcgat acgaatcagc tggttgtatt tagcaacacg gtcagaacgg ctcatagaac
     1021 cagttttgat ctggcctgca gcagtaccaa cagccagatc agcgatggta gcatcttcag
     1081 tttcaccaga acggtgagag ataactgcag tgtagccagc atctttcgcc atcttgattg
     1141 cagccagagt ttcggtcaga gaaccgatct ggttgaattt gatcaggatg gagttagcga
     1201 tacctttttc gataccttct ttcaggatct tggtgttggt tacgaacagg tcgtcaccaa
     1261 ccagctggat tttgtcgccc agaactttgg tctggtatgc gaaaccgtcc cagtcagatt
     1321 cgtccagacc gtcttcgata gaaacgatcg ggtactgttt ggtcagttct tccaggaagt
     1381 gagtgaattc ttcagaggtg aacgctttgt tgccttcgcc agccagaacg tatttaccat
     1441 ctttgtagaa ttcagaagct gcgcagtcca tcgccagagt gatgtctttg cccagttcat
     1501 aaccagcagc tttaacagct tcagcgataa cagccagagc ttcagcgttg gaacccaggt
     1561 tcggcgcata gccaccttcg tcaccaacag cagtgttcat gcctttcgct ttcagaactt
     1621 ttgccaggtg atggaaaact tcagaaccca tgcggatggc ttctttcaca gttttcgcgc
     1681 caaccggctg aatcatgaat tcctggatat caacgttgtt gtcagcgtgc tcaccaccgt
     1741 tgatgatgtt catcatcgga accggcatgg agtatttgcc cggagtgccg ttcagttcag
     1801 cgatgtgctc gtacagcggc atccctttag cggcagcagc agctttggcg ttagccagag
     1861 atacagccag gattgcgttc gcgccgaatt tggatttgtt ttcggtgccg tccaggtcga
     1921 tcatgatctt gtcaatgcca gcctgatctt tagcatcttt gccaatcagc gcctgagcga
     1981 tcgggccgtt taccgcagca acagctttgg ttacgccttt acccaggaaa cgggatttgt
     2041 cgccatcgcg cagttccaga gcttcacggg aaccagtaga agcacctgac ggagcagctg
     2101 ccataccgac gaaaccaccc tccagatgta cttcggcttc aacagtcggg ttaccacggg
     2161 agtcgatgat ttcacgaccg atgattttta cgattttgga cattaggttt tcctcaagtc
     2221 actagttaaa ctgaaactcc agacaaacaa cgcgtaccca gggtacgcgt tgccgttcta
     2281 acttttttac ttacttcgcc tgacgtttct ggaactcgct ggcggctttc acaaagcctg
     2341 caaacagcgg gtgaccatca cgtggagtag aagtaaactc cggatggaac tggcaagcca
     2401 cgaaccacgg gtgattcgga acttcgatga tctcgaccaa ctgatcatcc ccggaacggc
     2461 ccgcaacgcg cagacctgca tcttcaatct gtttcaacag catgttgttg acttcgtaac
     2521 ggtgacgatg acgctcaaca attgtcggcg cattgtacag ctggcgaacc aggctatcgt
     2581 caaccaactg gcactgctgt gcgccgagac gcatggtacc gccgagatcg ctcttctcgc
     2641 tacgaacttc aacgttgccg ttttcatcgc gccactcggt aatcagcgcc acaaccgggt
     2701 acttacagtc tggcacaaat tccgtagagt tggcgttctc catgttggca acatggcgag
     2761 cgtaatcaat taacgccacc tgcataccca ggcaaatgcc cagataagga atattgttct
     2821 cacgcgcaaa acgcgcggtc gtaatcatgc cttctacgcc acgatagccg aaaccgccag
     2881 gtacgaggat tgcgtccaga cctttaagga tttcaacgcc gcgcgtttca acatcttgtg
     2941 aatcgatcag tttgatgttg acgctgacac gattcttcag cccaccgtgt ttcagtgctt
     3001 cgatcactga tttataagca tccggcagtt caatgtactt gccgaccata ccgatggtga
     3061 cttcacttac cgggttcgct tcttcgaaga taacctgttc ccattcggac agattcgctt
     3121 ccgggcagtt taagctgaat cgtttacaaa tataatcgtc cagcccctga gatttcaaca
     3181 ggcccggaat tttatagatg gaatcgacgt ctttcagaga aataaccgct ttttccggaa
     3241 cattacagaa caatgcaatc ttcgcacgtt cgttcgccgg aacggcgcga tctgaacgac
     3301 aaatcaggat gtcaggctgg ataccgatgg agagcagctc ttttacagag tgctgagtcg
     3361 gtttggtttt gacttcacca gacgccgcca tgtacggcac cagcgtcagg tgcataaaca
     3421 gggtgtgctc acggccaatt tcaacagcca tctggcgaat cgcttcgagg aacggcaggg
     3481 attcgatatc acctacagta ccgccgattt cgaccagtac tacgtcatga ccttcgccgc
     3541 cttccagcac acgctctttg atagcgtttg tgatgtgcgg aataacctgt acggttgcgc
     3601 cgaggtagtc gccgcggcgt tctttacgca gaacgtcaga gtagatacga cccgtggtga
     3661 agttgttgcg gcggctcatt ttggtacgaa tgaaacgctc gtagtgcccc aggtccaggt
     3721 cggtttcagc gccgtcttca gtaacgaaca cttccccgtg ttggattggg ctcatagtac
     3781 ctggatcgac gttgatgtac ggatccagtt tcatgatggt cacattgagg ccacgggctt
     3841 caagaatggc tgcgagggag gctgcggcaa tgcctttacc cagagaggat acgaccccgc
     3901 cggtcacaaa aatatagttc gttgtcatgc tgaacctgag aagttaggtt gaaaagacga
     3961 tggaataacc aggacgggaa agcagtatac ccgaacatga cctgtgccac aaactttcat
     4021 tatccctcct cttcgccagc gcactattga aatcaggagt gagaaaatag cccctttggg
     4081 gtaaatgttt ttgacgcaaa tcaagcgctt gtcatttaaa aaatgacaca aatggcgctt
     4141 gaccgcgtaa ttcccttaga gatcaatttc ctgccgtttt acctgttgcc agacttcttc
     4201 cattgtttcg aggtcaacac ctgtcatttc cagtccacgc gcggcaacaa tacgctccac
     4261 ttcgcgaaaa cgacgctcga atttttcgtt cgctttttgc aatgcgattt ccgcttttgt
     4321 ccctaaatga cgagccagat tgaccgtggc aaacagcagg tcacccattt cctcctccag
     4381 tttagcctgg tcgacaacag cctgccgcgc ctcgtacatc acctcgtcga tctcttcgta
     4441 gactttatca accaccgggc caagcgtcgt ccagtcgaag ccaacgttgg cgcaacgttt
     4501 ctggattttt tgcgcccgca ttaaagccgg taaactacga ggaatatcgt ccagcgccga
     4561 atgctgcgct ttctgcgcgc gctcttcggt tttgatttgc tcccaacggg caagcacttc
     4621 actactgttt tcggcagaac tatcagcaaa aacatgcgga tggcgacgct ctaatttatc
     4681 gctaatagca gcgcaaatat cattaaagtc aaagcgccct tcttcctgag ccatttgcgc
     4741 gtaaaacacc acctggaata gcagatcgcc cagttcaccg cgaagatcgt caaaatcttc
     4801 acgggcgatg gcgtccagca cttcgtaggt ttcttcaagg gtgtaaggcg caatggtggc
     4861 aaatgtctgc tctttatccc acgggcagcc gttttccgga tcgcgcaggc gctgcataat
     4921 agtgagcaaa cggtcgattt gattcattga attgtcctga aaattgcggg tctgtcaggt
     4981 gtaatacctt cgagtaaaag atatcgctgg atacggctga cgccatatcc agctatgtaa
     5041 gactaactcc cgtgcaaccg acgcgcgtcg ataacatccg gcacttggtt gagtttaccc
     5101 agcacgcgac ccagcacctg caggttgtaa atctcaatgg tcatgtcgat ggtcgccagt
     5161 tgctgtttgg tgtcgctacg gctggcaacg ccaagtacgt tcaccttctc gttggcgaga
     5221 atggtcgtga tatcacgtaa caacccacta cgatcattag ccaccacgcg gaccaccagc
     5281 gaatatccgg cggagtagct ctcaccccat accgcgtcaa caatgcgttc tggcgcatgg
     5341 gagcgcagtt ccgccagttg ttcgcaatcg gcgcggtgta ctgaaatacc gcgtccctgg
     5401 gtaatgaagc cgacaatctc atctccagga atcggctggc agcagcgcgc gatgtggtgc
     5461 atcaggttac cgacaccttc aaccaccaca cgaccgttat ctttactgcg gttttgcggt
     5521 gtgtagcttt tttgctgaag ctgtttcagc gcggcggcgt cctgctcttc ggcactcggc
     5581 ttattaaatt gcgattgcag gaagttcacc atctgattga gacggatatc cccgccacca
     5641 atcgccgcca acaactcgtc gacatcattg aagttgtaac gcggcagcag atgtttttct
     5701 gcttctttca ggctgatccc cagatgttcc agctcgtcgt caaggatttg ccgcccagcc
     5761 agaatgtttt tgtcacggtc ctgtttacgg aaccaggcgt gaattttcga gcgcccacgg
     5821 ctggttgtga cgtaaccgag gtttgggttt aaccagtcac ggctggggtt cggctgtttc
     5881 tgggtgataa tttcaatctg gtcgcccatc tgcagctggt aggtgaacgg cacaatgcgc
     5941 ccgccaattt ttgccccgat gcagcggtgt ccgacatcac tgtggatgtg gtaagcgaag
     6001 tccagcggcg ttgatcccgc aggcaaatca acgacatcac ctttcggcgt aaagacgtac
     6061 actcggtcgt caaagacctg actacgtact tcgtcgagca tttcgccgga atcagccatc
     6121 tcttcctgcc acgcaatcag tttacgcagc caggcaatcc ggtcttcatg tcccgaacgt
     6181 gcgccgccag cagccgcgcc ctctttatat ttccagtgcg cagcaacacc caactctgca
     6241 tcttcatgca tctgtttggt gcggatttgg atctcaacgg tttttccacc cggccccaga
     6301 accacggtat gaatagactg ataaccgttt ggtttcgggt tagcgacgta atcgtcaaac
     6361 tcatccggca ggtggcgata gtgagtgtgc actatcccca gtgcggcata acaatcctgt
     6421 aaacgctcgg cgacaatacg taccgcacgc acatcaaaca gctcatcgaa ggcgaggttc
     6481 tttttctgca ttttgcgcca gatgctgtag atgtgtttcg gacgaccata cacttcagct
     6541 ttaacgcctt cagctttcat ctcagcgcgc agatggccga caaactcttc gatatagtgt
     6601 tcgcggtcga gacggcgttc atgcaacagt tttgcgatgc gtttgtattc ggtcgggtgg
     6661 agataacgga agcagtaatc ttccagctcc catttcagtt gcccaatccc aagacggttt
     6721 gccaacggcg cgtagatatt ggtgcactct tttgccgcca gtacgcgttc atcttccggc
     6781 gcatctttta cttcacgcag atgagcaata cgctccgcca gtttgatgac cacgcagcga
     6841 aaatcatcga ccatcgccaa taacatccgg cgaacgttat cgacctgttc ggaggaaaca
     6901 gaatcagtgt gcgtcgcttt cagctggcgg atcgccgcca tatcacgcac gccgtgaata
     6961 aggttaacga ccgacttacc gacgctctca cgcagcacat cttcgctgac tacgttggca
     7021 tccgccagag ggaaaagcag cgccgcccgc agcgtgtcaa tgtccatact taatgtcgag
     7081 aggatctcca ccatctcaac accacgccac aataacagac tggcatccgg atgcccctgc
     7141 gtctgttgca gacaatacgc ccaggtttcg gctaagcact cacacgactt ctggctggta
     7201 atacccagac ttgcgatcca tttttccgga tcaaattcac cagccttatt gatatgtgca
     7261 cttcttaccg caaccatcgt cctctccttt agggaccaga cctgccgaaa tcggcaaatc
     7321 gcaactattt aacgcgcgag aaaagtacca tcgattccag atgtcccgtg tgtgggaaca
     7381 tatccagcat cgccagtcgc gcaatggtat atcctgcttt taataacgct tcgctatccc
     7441 gagccagcgt tgcagggtta caggatacat aaactatacg aataggttcc agttttataa
     7501 tttgctgcat aacacctgcg gcacctgctc gcgccgggtc cagcaacact ttatcgaagc
     7561 cgtttttcgc ccacggctgc tttgtgacat cttcttcaag attttcatga taaaacgtca
     7621 cattctgtaa gccattaaga cgcgcattct gctggccttt ttccaccagc gccggaacac
     7681 cttctacacc caccacactg gctgcttgtg tcgccaatgg cagtgtaaag ttgcccatac
     7741 cgcagaacag atccagtacg cgatcttcag gttccacatc cagccattcc aacgcacgcg
     7801 ctaccatttt ttggttcaca cccgcattaa cctgaataaa atcgcggggg ctaaaagtta
     7861 agcgcaaccc gtttgagtca taccagggca tctcaccaga gacggtttcg agtatctcac
     7921 tatcgggggc gagatacaga tccaggcctt cagaatgcga aaagcgttcc agtttttcgc
     7981 gatctgccga acttagcggt gcggtatggc gcaaaatcat cagcgtgccg ctggttgcct
     8041 gtaccagttc aacatgacca agatggcgca tagcttgtaa gctgcccagg catgccctga
     8101 ctttgggcag caatgcttca agttggggcg ctaaaatggg gcattgttta acgtcgacaa
     8161 tgtcactgga gcccgctttg cgaaacccca tctgaagttg ctgtgttttc ggtaagtaat
     8221 tcagacttaa acgcgcgcga cggcgatagc cccagggaac atcggcgatc acttcagaga
     8281 cttcgtgttt cattaatcgg gcgagtgccg cacttttgct tcgctgctgt aaatccacgc
     8341 tggcgtgttg ttgctgacag ccgccgcata cgccaaaatg aggacagcgt ggcgtttcgc
     8401 gttccgggct atcgcttaac cggcgtacga ctttagcgcg ggcatactgt tttttatctt
     8461 cagtaacagt aacttccgcg ttttcctgcg gtaataatcc ggggataaat agcgttttgc
     8521 cgttatgtcg cgccacgccc tgaccaaaag agtcgaggtc gttgactgaa acggttatga
     8581 tctgacgcgt cgtcgtgcgt cgttttgcag agtagaattg cgccattggc gagactttct
     8641 caatttaaca gtgtgacctt aattgtccca taacggaact ccatgaccaa ctacagcctg
     8701 cgcgcacgca tgatgattct gatcctggca ccgaccgtcc ttattggttt attgctgagt
     8761 atctttttcg tcgtgcatcg ctataacgac ttgcagcgtc aactggaaga tgccggtgcc
     8821 agcattattg agccgcttgc agtttctact gaatatggca tgagcctgca aaatcgcgaa
     8881 tctatcggtc agttaataag cgtactgcat cgtcgccatt ccgatattgt tcgcgcgatt
     8941 tcggtttatg atgaaaataa ccgactcttt gtcacctcca attttcatct tgatccctca
     9001 tcaatgcagc tcggcagcaa cgtgccgttt cctcgccagc tcactgtcac tcgtgacggc
     9061 gatattatga tcctccgcac gccgattatt tctgagagtt actcccccga cgaatcgccc
     9121 agtagcgatg ccaaaaatag tcaaaatatg ttgggatata ttgcgcttga gctggatctt
     9181 aaatcggttc gcttgcagca atataaagag atctttattt ccagcgtgat gatgctgttt
     9241 tgtatcggta ttgcgcttat ttttggctgg cgcttaatgc gcgatgtaac cggtccgatt
     9301 cgcaacatgg tgaataccgt cgaccgcatc cgtcgcgggc aactcgacag ccgggtggaa
     9361 ggatttatgc tcggcgagct ggatatgctg aaaaacggta tcaactcgat ggcaatgtcg
     9421 ctggctgctt atcacgaaga gatgcagcac aatatcgacc aggcgacgtc cgatctgcgt
     9481 gaaacgctgg agcagatgga aattcagaac gttgagttag atctggcgaa aaagcgcgcc
     9541 caggaagcgg cgcgtattaa atccgagttt ctggcaaata tgtcacacga gctgcgtaca
     9601 ccactgaatg gtgttattgg ctttacccgc ctgacgctga aaacagaatt aacaccaacg
     9661 cagcgcgatc acctgaatac gattgaacgt tcggcaaata atttgctggc aattattaat
     9721 gatgttctcg acttctcgaa actggaagca ggtaagctga ttctggaaag tattccattc
     9781 ccactacgca gcacgctgga tgaagtcgtt actctgctgg cacattcttc tcacgataaa
     9841 gggttggaac tgacgctcaa tattaaaagc gacgtgcctg ataacgtgat cggcgaccca
     9901 ctgcgattac agcaaatcat cactaacctg gtggggaatg caattaaatt caccgagaat
     9961 ggcaacattg atattctggt agaaaaacgt gcgctgagta ataccaaagt gcagattgaa
    10021 gtgcagattc gggataccgg cattggtatt cctgaacgcg atcaatcgcg cttattccag
    10081 gccttccgac aggctgatgc cagtatttcc cgccgtcatg gtggcaccgg tctggggctg
    10141 gtgattacac aaaaactggt taatgaaatg ggcggcgata tttcgttcca tagccagccg
    10201 aatcgcggtt caactttctg gttccacatt aatctcgatc tgaacccgaa cattattatc
    10261 gaagggccat ccacccagtg cctcgccggt aagcgcctgg cctatgtcga accaaactcc
    10321 gcagcagcgc aatgcacgct ggatatttta agtgaaacgc cgctggaagt ggtttatagc
    10381 ccaacgttct ccgcgctgcc tcccgcgcat tacgacatga tgttgttagg catcgcggtg
    10441 accttccgcg agccgctaac aatgcaacat gagcgattag cgaaagcggt atcgatgacc
    10501 gatttcctga tgctggcact tccttgccat gcacaagtca atgcagaaaa actcaagcaa
    10561 gatggtatcg gcgcgtgtct gctgaaacca ttaacaccta cgcgcctgtt gcctgccctg
    10621 acggaatttt gtcatcacaa acaaaacacg cttttgcctg taaccgatga aagcaagctg
    10681 gcaatgacag tcatggcagt tgatgacaac cccgctaacc tgaaacttat cggcgcattg
    10741 ctggaagata tggtgcaaca tgtggaactt tgcgatagcg ggcatcaggc ggttgaacgg
    10801 gcgaaacaga tgccgttcga tttgatctta atggatattc aaatgcctga catggatggc
    10861 attcgggcct gcgagctcat ccaccagctc ccccatcagc aacaaacgcc ggttatcgcg
    10921 gtaacggcgc atgcaatggc cgggcaaaaa gagaagctgc ttggcgcagg gatgagcgat
    10981 tatctggcga aaccgattga agaagagcga ttgcataatt tgctgttgcg ctacaagcct
    11041 ggcagcggta tttcctctcg cgtcgtgacg cccgaagtca acgaaattgt ggtgaacccg
    11101 aatgcgaccc tcgactggca actggcacta cgccaggcag caggaaaaac cgatttagcg
    11161 cgcgatatgc tgcaaatgtt actcgatttc ctgcctgaag ttcgcaacaa agttgaggaa
    11221 cagctggttg gagaaaaccc ggaaggcctg gttgatttga ttcataaact gcatggcagt
    11281 tgcggctata gcggtgtgcc gcgtatgaag aatctctgcc aactgatcga acaacagcta
    11341 cgtagcggta ctaaagaaga agatttggag ccggagctgc tggaactgtt ggacgagatg
    11401 gataatgtcg cgcgcgaagc cagcaaaatt ctcgggtaat ggacgtcttg tcgagtagca
    11461 atcggctttt tatgaagcag gtttcagatt ggtgacaaag tgtctgtgtt tatgccggat
    11521 gcggcgttaa cgctttatcc ggcctacaaa accaattaaa ttcaatgaat taaaaaaatc
    11581 atgtaggcct gatacgtagc gcaacaggca attttgcgtt tggcatcagt ctcaaaccgg
    11641 ctccagatag agccggtttt ggttttctgt cttaacgcac catgcacggg cgcttgttat
    11701 cgaacgtcca gccaggaatc aggtactgca ttcccatcgc atcgtcacgc gcaccaagcc
    11761 catgtttctg atacagctcg tgcgctttca tcacctgatc catatcgatt tctacaccca
    11821 gccccggttt ttctggcact tgtaccagcc cgcctttgat ctcaaacggt tctttggtca
    11881 ggcgctgatt gccttcctgc caaatccagt gcgtatcaat agcagtaatt ttacccggtg
    11941 cagcggcggc aacatgggta aacatcgcca gggaaatatc gaagtggttg ttagagtgtg
    12001 aaccccagat caggccaaat tcatggcaca tttgcgccac acgtaccgaa ccttgcatcg
    12061 tccagaaatg cggatccgcc agcgggatat caacggattg taacgagagc gtatggccca
    12121 tctgccgcca gtcggtggcg atcatattgg ttgcagtcgg caggccagtc gcgcgacgga
    12181 actctgccat cacttcacgc ccggagaaac cttgctccgc accacacgga tcttctgcat
    12241 aagccagcga acctttcagg tatttaccaa ttttaatcgc ttcgttcagc gaccaggcac
    12301 cgttaggatc gagcgtaata cgcgcctgcg ggaagcgttg cgccagtgcc acaatagact
    12361 cggcctcttc ttccccggcc agtacaccgc ctttcagttt gaaatcgttg aagccatatt
    12421 tttcatacgc cgcttccgcc aggcgcacca ccgcatccgg cgtcatcgct tcttcatgac
    12481 gcaggcgata ccagtcgcat gagtcatccg gctggctttg atacggcaga ggtgtggctt
    12541 tgcgattacc gacgaagaac agataaccga gcatttcgac ttcgctacgc tgttgaccat
    12601 cgcccagcag cgatgccacg tttaccccca gatgctgccc cagcagatcc agcattgccg
    12661 cttctatccc ggtaactaca tgaatagtgg tacgcaaatc aaatgtctgc aaaccgcgcc
    12721 caccagcatc acgatcggca aaagtattac gcaccagcgt cagaacgttt ttgtattcac
    12781 ccagcgtttt acccactacc agcggaatcg catcttccag cgttttacgg attttctcgc
    12841 cgccgggaat ttcccctacg ccagtgtgac cagaattatc tttgataatc acaatattac
    12901 gcgtgaagaa cggtgcgtgt gcaccactca gattcatcag catactgtca tggcccgcca
    12961 ccgggatgac ctgcatttca gtaacaacag gcgtcgtaaa ttgagaactc atatttatgt
    13021 cctttttcag aatcaatgac ggccgaaaac gggacgttta cggtcaaagg tccagccggg
    13081 gatcaggtac tgcatcggac ctgcgtcgtt acgcgcaccg ccaggcagac gtttataggc
    13141 ctcatgtgcc ttttgtacct gttcccagtc cagttccacg cccagaccgg gcgcatcagg
    13201 aacggcaatt tttccgtttt taatctccag cggattttgg gtcaggcgac agtcgccctc
    13261 ctgccaaatc cagtgggtat cgatagcggt aggattaccc ggtgccgccg cgcccacatg
    13321 ggtaaacatc gccagagaga tatcgaaatg gttattagaa tggcagcccc aggtcagccc
    13381 ccagtcgtcg caaagctgcg ccacacggac tgcaccggaa agcgtccaga agtgcggatc
    13441 ggcaagtgga atatctaccg cattgagcat caccgcatga cccatttcgc gccagttggt
    13501 ggcgatcatg ttagtcgcga cgggcaagcc ggtcgcccgc cgaaactccg ccatcacttc
    13561 acgtccggag aagccctgtt ctgcgccgca tggatcttcg gcataggtaa gaacatcatt
    13621 cagccctttg cacaaagaaa tggcttcatc aagcagccag gcaccgttgg gatcaacggt
    13681 aatccgcgca tccgggaagc ttttcttcaa tacacgaaca gtgtcgattt cttgctcgcc
    13741 aggtaacacc ccgcccttaa gtttgaaatc tttaaagcca tagcgatcct gtgaggcttc
    13801 cgccagacgc acaacggctt cgctgttcat cgctttctga tgtcgcaact gataccactc
    13861 atggttgccc ggtgtatttt ccagataagg aagatcggtt ttagtccgat caccgatata
    13921 aaacagataa ccgaggacgg tgatagcatc gcgttgcttg cctggcccta acagttcgca
    13981 gaccggaaca ttcagcgcct tacctagcag gtcaagcaag gcggcttcca gcgccgccac
    14041 ggcattaacg cgcaattcaa aagtccaggc acctttgccg aaggtatcaa aatcggctgg
    14101 ctgattacct ttatgcacct gctgaaccac tttattcagg cgcgcaactt cctggcccag
    14161 caccatcgga atagcatcga ccaatgtctg ataaatcacc tctccgcctg gcgcttcacc
    14221 aatgccggta tgcccggcgt tatcggtgag tagcacaata ttgcgggtga aatatgcgtt
    14281 atgcgcccca ccaatattaa gcaacatgct gtcgtgcccg gcgaccggaa tgactttcat
    14341 atcagtaata acaggactgg attgtgtcgt catcttattg ccccgcgaca ggtttcagct
    14401 cgatacgctt gatatcgccc accagcacca ggtagctcag taccgcgatt aaggcatgaa
    14461 caccaacata aatgagcgcc ccattaaagg agccagtcgt gccaacgata taaccaattg
    14521 cgattggcgt gacgatgcca gaaatgttac cgaacatgtt gaacaggcca ccggaaagac
    14581 cgctgatctc ttttggcgcg gtatctgcca ttactgccca acccagcgcc ccgatgccct
    14641 taccgaagaa ggccagcgcc ataaagccga tgatcatcca ctcaacgttg acgtagttgc
    14701 agaacaccat caccatcgac agcaacatgc ccattacgat cggtgtttta cgcgcaatgt
    14761 tcagcgatcc cgtgcggcgc atcagccagt cggaaataat cccacccagc acaccgccga
    14821 taaaaccgca aaccgccgga acggaagcca caaatcccgc tttcagaatc gacatcccac
    14881 gcgcctgcac cagataaacc ggaaaccagg taatgaaaaa gtaagtcagg gcgttgatac
    14941 agtactgacc gatataaacg ccgatcatca tccgtgaccc gagcagctgt ttgatctgcc
    15001 cccacttcac gctgaacggc actttaactt tggtgttttg ctgatccata ttgatcagcg
    15061 cgccacccgc ggcgatgtac tccagctctt tctgatttac acccggatgt tgatttggct
    15121 cgtggatgac tttcaaccag ataaagctga tgacaatgcc cagaccgccc ataaagaaaa
    15181 agacgtgtga ccagcccact tcatgcgtca gccagcccat aatcggcgcg aagatcaccg
    15241 ttgcgaagta ttgagcggag ttaaaaatcg acaccgccgt tcccctttcc tgcgccggaa
    15301 accaggccgc aacaatgcga ctattgccgg ggaaagatgg cgcttcagca agcccgacca
    15361 ggaagcgcag cgtaaacagg gcaacgataa tgccgaatcc actaaagata tcgacgaagc
    15421 cttgcagcaa ggtaaacatc gaccagataa agatcgacca gaagtagacg cgttttgaac
    15481 caaaacgatc cagcaaccaa ccaccaggga tctgcccgat aacataagcc catgagaagg
    15541 cagagaacac atagcccatt cccacgggat caaggccgat atctttggcc atttccgaac
    15601 cggcaataga aagcgtagcg cggtcgccgt agttgaagga tgtgacgata aacaacatca
    15661 ccactatcca gtaacgagca ttcgtgcgtt tttccacact gctcgcagcc tgacttaaag
    15721 aactcattgt tgcactcctg aaaattcgcg ttagccacgc tcactctgga ctgcgacatc
    15781 gccaggaaat cagaggtgac gtagggtgtt ttttgccgtt tttataggtc gttcgccgaa
    15841 tacggcgcgt gtttatatct ggcaatagca gtataaaaag cgcgccatag cggctcaccg
    15901 tgcaacaaca caacattaat gcgttcaatg aggcccgatt ttggcattag ccctggacgg
    15961 tggaatccac ttcacggaaa tgaaaacaag aacaagaaag gaagggttaa aacgaagaaa
    16021 taaagaagag tatgaaatgg atcgcttgac tccaggcaaa cgccagtaaa aatccgcgct
    16081 atgaagcagt ttttactggc atttgcctga aaagattcga ttcagcaccg ctaaaacgac
    16141 atttaccgct cgctgaacat atcaggacaa cagcgtgccc cactgttcga cccacagatt
    16201 tgattccgtt tccggttccg ggttctcact ggcgtcaatc aacagcattt cgccaacccg
    16261 ctgagcgctc tgttcctgca acaaggcatc gaactgtttg ccgccattgc agaaatttac
    16321 ataactgctg tcgccgagcg caatcacgcc ataacgcaga ttcggctgga agcccagact
    16381 atctttgatt ccctgaaaga gcggcacaat gctatcagga aggtcgccct gcccggtcgt
    16441 ggacgtaacc accagaacat acttatcctg atagggcagc cagtcgctta attcaggatc
    16501 ttcaaatacc gttgctttgt ggccctgtgc ggtcagaatt gcttctgctt cttccgccac
    16561 taacagcgaa tttccgtaca tggtgccgac aaaaatacca atttccgcca taccgttttc
    16621 tccctggatt aggaacttat ctctccatcc tgacccgatg gcgcactgaa ctcaaccctt
    16681 tcattttcag gaagcagacc gcgccagcca aattgtgata acgcctgcat ccaggtgtcg
    16741 tccaggcccg cgtgaatagt cagcggctcg ccagtaaaag gatgcgtcaa tgacaactga
    16801 ctggcatgca gcattaagcg ctgaaggcca aaatgctcag caccgctgcg attctggcgt
    16861 aaatcgccat gtttgctatc gccaataatc ggatgacgca aatgggcaag atgtcggcga
    16921 agctgatgtt tgcgtccggt tttcggctcc agttccacca ggccgtagcg cgtggtcggg
    16981 taacgtccgg tcgctaccgg catttctacg gtcgccagac cgcgataatg cgtcactgct
    17041 ggctgcgggc ctttatcttc gcgggcaaat ttatcagcga ttttgtccag ttcttccacc
    17101 agtggataat ccagcaccgc ttcttccatc aaccagccgc gcacaatcgc atggtaacgt
    17161 ttctggattt ggtgctgttc aaactgttgt gccagcagcc gtccggcctc gctggataat
    17221 cccatcaaca acacaccaga agtgggtcgg tccagacgat gagcagtaaa aacatgctgg
    17281 cctatctggt cacgcacggt ttgcatgacc actactttct cgtcgcgatc cagccagctg
    17341 cggtgaacca gccagccgga gggtttattt accgcaacca gccattcatc ctgatagagt
    17401 atttccagca ttagctcgca tcatccgcaa aaagagcatc cagtttttcc aactcagcca
    17461 gaataagcgc gcgttgcgga tggtccgtcg ccagcgccat ttcataatag ggagcaacgg
    17521 caaaagcgcc cggtaacggc tggttgttat ttaacaaatc gtgcattcgc gggatcagca
    17581 cccactgcaa ccactccagt ggttccatgg tgtccataaa gaacggttgg gtactattaa
    17641 attgatgcgg ctggggttca tcgtttcgcc agtgctgatg ttcacgcagt aacgcttcaa
    17701 gcgtctgcaa ctggagacga acgcggtcat gagtggtcat aaagcaacct caataagaaa
    17761 aactgccgcg aaggatagca ggaataaaaa aagggagcac tgtattcaca gcgctcccgg
    17821 ttcgtttcgc agcattccag ctacttttgt tgctccctgc tcatccttga caacttttcc
    17881 tctggccttg cggccaatcg ttcatcctga actattgctt cctgctcaca ccaccccgat
    17941 gtgatacttc atcctgaagt gtccctggcc ttcctgaccc accgaatcat cctgaccggt
    18001 tctcattctc catcctggag gtgtccttta acgcgtcctg cgtcatcctc ttcgcttcat
    18061 ccagaagcct ttccctgaaa caccatcctg gtgtgtcctg cagaagtgtc atcatcctga
    18121 tgttcacttc gttgtctgac tccctgtcga cgaagataga atcgtctttt tcgcgaagtc
    18181 ttacaaggcg cttacagaca atgccttaag gaaaatttca tatgaaagtt aagaattaac
    18241 aatcaacatt ttgattttaa atgaaaataa cgattcttat ctttttaagt attcgcttat
    18301 ttcctggcga tctcctacag agtctgtaag agatctctca caaccagatg gggtatttgg
    18361 attacagaag aggcttaagt tgattaagga attccgcaag atttgaggct aaatgcgtcc
    18421 gtttacgtgt tcccagcgtc tctttgcaca cttcaccgga gagattacac accgagatga
    18481 cctctaactc ttcttccagc gtcgcgataa atagcgtggg tggcagtttt agccttttct
    18541 gagtcaccag atgaccaatc agattttcct gcacccgacg gaagtcgtct tcactccagg
    18601 tttgcagcaa tgtcagtttg atatcgccaa actgcgcgtg catatccccg gcaaactgag
    18661 tcgtataaaa ggtatgaatt gtgggttgta tcacaacatc aaaagcgcgt tcaaccgcgt
    18721 ttacattttg ttccccggta aacggctggg gttgccagta tacggcatct tcagtagttg
    18781 aaataatgca cggtgaagga acgccgtaca gttcctcact taacggccag cttttatgct
    18841 cttcatgcca cgcatcgcag tagcgtgcag taaaatcttt cagggcttgt gcggtcaaat
    18901 cgtccactaa tttctctctt cacttaaacc agatacactt gtcgtttagt ttatctggtt
    18961 tatgacggtg aaacatgtct tcttatgcaa accatcaggc acttgcgggc ctgactcttg
    19021 gaaaatcaac cgattaccgg gatacctatg acgccagcct actgcaaggc gttccacgca
    19081 gcctgaatcg cgacccgctg ggtctgaaag cggataacct gccttttcac ggtacggata
    19141 tctggacgct gtatgaactt tcctggctga atgcgaaagg tttgccgcag gtcgctgtcg
    19201 gtcatgttga acttgattac accagcgtaa atctgattga gtcgaagagt tttaagctct
    19261 atctcaacag ttttaaccag acgcgtttta ataactggga tgaggtgcgc cagacgctgg
    19321 agcgcgactt aagcacttgc gctcagggta aggttagcgt ggcgttatat cgtcttgatg
    19381 aactggaagg ccagccgata ggtcatttca atggcacttg cattgatgac caggatatca
    19441 ctatcgataa ctatgaattc actactgact atctggagaa tgccaccagt ggtgaaaaag
    19501 tagtggaaga gacgctcgtc agccacctgc tgaaatcaaa ctgcctgatc acccatcaac
    19561 cagattgggg ttcgatccaa attcagtatc gtggacgcca aattgacaga gaaaaactgc
    19621 tgcgttacct ggtctctttc cgccatcaca acgagttcca cgaacagtgc gtggaacgcc
    19681 tctttaatga cctgttacgc ttctgccagc cagagaaatt gagcgtttac gcacgttata
    19741 cccgtcgtgg cggtctggac attaacccgt ggcgcagtaa tagcgatttt gtcccatcga
    19801 ccacaagact ggttcggcaa taaatttttt ctcaattttg cgtgctggat tcacgcagaa
    19861 ggttgtgaaa ggtcatcagg cagggctatt gtaatcaaag ggaatgacga tattcgtccc
    19921 ataaggagtt tttcttgatt acacatatta gcccgcttgg ctccatggat atgttgtcgc
    19981 agctggaagt ggatatgctt aaacgcaccg ccagcagcga cctctatcaa ctgtttcgca
    20041 actgttcact tgccgtactg aactccggta gtttgaccga taacagcaaa gaattgctgt
    20101 ctcgttttga aaatttcgat attaacgtcc tgcgccgtga acgcggcgta aagctggaac
    20161 tgattaatcc cccggaagag gcttttgtcg atgggcgaat tattcgcgct ttgcaggcca
    20221 acttgttcgc ggttctgcga gacattctct tcgtttacgg gcaaatccat aacaccgttc
    20281 gttttcccaa cctgaatctc gacaactccg tacacatcac taacctggtc ttttccatct
    20341 tgcgtaacgc tcgcgcgctg catgtgggtg aagcgccaaa tatggtggtc tgctggggcg
    20401 gtcactcaat taacgaaaat gagtatttgt atgcccgtcg cgtcggaaac cagctgggcc
    20461 tgcgtgagct gaatatctgc accggctgtg gtccgggagc gatggaagcg ccgatgaaag
    20521 gtgctgcggt cggacacgcg cagcagcgtt acaaagacag tcgttttatt ggtatgacag
    20581 agccgtcaat tatcgccgct gaaccgccta acccgctggt caacgaattg atcatcatgc
    20641 cggatatcga aaaacgtctg gaagcgtttg tccgtatcgc tcacggtatc attatcttcc
    20701 ctggcggtgt gggtacggca gaagaattgc tgtatttgct gggaatttta atgaacccgg
    20761 ccaataaaga tcaggtttta ccattgatcc tcaccggccc gaaagagagc gccgactact
    20821 tccgcgtact ggacgagttt gtcgtgcata cgctgggtga aaacgcgcgc cgccattacc
    20881 gcatcatcat tgatgacgcc gctgaagtcg ctcgtcagat gaaaaaatcg atgccgctgg
    20941 tgaaagaaaa tcgccgcgac actggtgacg cttacagctt taactggtca atgcgcattg
    21001 caccagattt gcaaatgccg tttgagccgt ctcacgagaa tatggctaat ctgaagcttt
    21061 acccggatca acctgttgaa gtgctggctg ccgacctgcg ccgtgcgttc tccggtattg
    21121 tggcgggtaa cgtaaaagaa gtcggtattc gcgccattga agagtttggt ccttacaaaa
    21181 tcaacggcga taaagagatt atgcgtcgta tggacgacct gctacagggt tttgttgccc
    21241 agcatcgtat gaaattgcca ggctcagcct acatcccttg ctacgaaatc tgcacgtaat
    21301 ctccgctccc ccggtgacgt ttgttgccgg gttttctcgt ttttggtcac ttactcatca
    21361 actcatttca tttgttatat aaatgtttct taccaccc
//
LOCUS       DAPGOF010000080        21377 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.080, whole genome shotgun sequence.
ACCESSION   DAPGOF010000080 DAPGOF010000000
VERSION     DAPGOF010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.080"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            91..327
                     /gene="lpp"
                     /locus_tag="RHV13_002581"
     CDS             91..327
                     /gene="lpp"
                     /locus_tag="RHV13_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HDV1242106.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(390..1394)
                     /gene="ldtE"
                     /locus_tag="RHV13_002582"
     CDS             complement(390..1394)
                     /gene="ldtE"
                     /locus_tag="RHV13_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HDV1242107.1"
                     /translation="MKRASLLTLTLIGAFSAIQAGWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVAVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(1543..1959)
                     /gene="sufE"
                     /locus_tag="RHV13_002583"
     CDS             complement(1543..1959)
                     /gene="sufE"
                     /locus_tag="RHV13_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HDV1242108.1"
                     /translation="MAVLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            complement(1972..3192)
                     /gene="sufS"
                     /locus_tag="RHV13_002584"
     CDS             complement(1972..3192)
                     /gene="sufS"
                     /locus_tag="RHV13_002584"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HDV1242109.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAGMITLAHQHGAKVLVDGAQAVMHHSVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALDYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            complement(3189..4460)
                     /gene="sufD"
                     /locus_tag="RHV13_002585"
     CDS             complement(3189..4460)
                     /gene="sufD"
                     /locus_tag="RHV13_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HDV1242110.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSMLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            complement(4435..5181)
                     /gene="sufC"
                     /locus_tag="RHV13_002586"
     CDS             complement(4435..5181)
                     /gene="sufC"
                     /locus_tag="RHV13_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HDV1242111.1"
                     /translation="MLSIKDLHVNVEDKAILRGLSLAVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            complement(5191..6678)
                     /gene="sufB"
                     /locus_tag="RHV13_002587"
     CDS             complement(5191..6678)
                     /gene="sufB"
                     /locus_tag="RHV13_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HDV1242112.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            complement(6687..7055)
                     /gene="sufA"
                     /locus_tag="RHV13_002588"
     CDS             complement(6687..7055)
                     /gene="sufA"
                     /locus_tag="RHV13_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HDV1242113.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            complement(7604..7792)
                     /locus_tag="RHV13_002589"
     CDS             complement(7604..7792)
                     /locus_tag="RHV13_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HDV1242114.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLNRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            complement(7892..8302)
                     /gene="menI"
                     /locus_tag="RHV13_002590"
     CDS             complement(7892..8302)
                     /gene="menI"
                     /locus_tag="RHV13_002590"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HDV1242115.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            complement(8299..11355)
                     /locus_tag="RHV13_002591"
     CDS             complement(8299..11355)
                     /locus_tag="RHV13_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDV1242116.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGLRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCARLDELIASHQAGVIGWQMCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPALWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            11744..12856
                     /gene="ydiK"
                     /locus_tag="RHV13_002592"
     CDS             11744..12856
                     /gene="ydiK"
                     /locus_tag="RHV13_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HDV1242117.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            13042..13149
                     /gene="rprA"
                     /locus_tag="RHV13_002593"
     ncRNA           13042..13149
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="RHV13_002593"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            13285..13641
                     /locus_tag="RHV13_002594"
     CDS             13285..13641
                     /locus_tag="RHV13_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HDV1242118.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            13741..14955
                     /locus_tag="RHV13_002595"
     CDS             13741..14955
                     /locus_tag="RHV13_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1242119.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            15182..16447
                     /locus_tag="RHV13_002596"
     CDS             15182..16447
                     /locus_tag="RHV13_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416206.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1242120.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVTNELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            16459..17325
                     /gene="ydiB"
                     /locus_tag="RHV13_002597"
     CDS             16459..17325
                     /gene="ydiB"
                     /locus_tag="RHV13_002597"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HDV1242121.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            17356..18114
                     /gene="aroD"
                     /locus_tag="RHV13_002598"
     CDS             17356..18114
                     /gene="aroD"
                     /locus_tag="RHV13_002598"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDV1242122.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVEMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            18260..19855
                     /locus_tag="RHV13_002599"
     CDS             18260..19855
                     /locus_tag="RHV13_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HDV1242123.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SDLAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQTQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEITDGKLNIVQEGRVKKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFPPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            19869..21020
                     /locus_tag="RHV13_002600"
     CDS             19869..21020
                     /locus_tag="RHV13_002600"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HDV1242124.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTQETGDPAYALALTEPGAGSDNNSATTIYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPEPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(21063..>21377)
                     /locus_tag="RHV13_002601"
     CDS             complement(21063..>21377)
                     /locus_tag="RHV13_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV1242125.1"
                     /translation="EQLSKKFRASVSYICHEFTKEYRISPINYVIQRRMTEAKWSLTN
                     TELSQAEISWRVGYENVDHFAKLFLRHVGCSPSDYRRQFKNCFAEQEILSEFPQPVSL
                     AG"
BASE COUNT         5202 a         5414 c         5221 g         5540 t
ORIGIN      
        1 tcccatcaaa aaaatattct caacataaaa aactttgtgt aatacttgta acgctacatg
       61 gagattaact caatctagag ggtattaata atgaaagcta ctaaactggt actgggcgcg
      121 gtaatcctgg gttctactct gctggcaggt tgctccagca acgctaaaat cgatcagctg
      181 tcttctgacg ttcagactct gaacgctaaa gttgaccagc tgagcaacga cgtgaacgca
      241 atgcgttccg acgttcaggc tgctaaagat gacgcagctc gtgctaacca gcgtctggac
      301 aacatggcta ctaaataccg caagtaatag tacctgtgaa gtgaaaaatg gcgcacattg
      361 tgcgccattt ttttgtctgc catttaccgc tactgcgtcg cgcgtaacat attcccttgc
      421 tctggttcac cattctgcgc tgactctact gaaggcgcat tgctggctgc gggagttgct
      481 ccactgctca ccgcaaccgg ataccctgcc cgacgataca acgctttatc gactaacttc
      541 tgatctacag ccttattgtc tttaaattgc gtaaagcctg ctggcagtgt gtatggcatt
      601 gtctgaacgt tctgctgttc ttctgccgat agtggtcgat gtacttcaac ataacgcatc
      661 ccgttaggct ccacggaata tttcaccggt tcgttgatca ctttcaccgg cgttcccgtc
      721 cgcacgctgg agaacaaggc tttaatatcc ggtgcattca tgcgaataca ccctgaactg
      781 acgcgcaaac cgacgctgtc cggcgcactg gtgccatgaa tgaggtattc gccattacca
      841 tgcgcgaggc gcagtgcgta acgtcctagc gggttatttg gtccggcagg aacgactggc
      901 ggtaatttaa taccacgctc cagtgaacgt tgacgaatgc ctgcagtagg cgtccaggtt
      961 gggttaggga ttttctgccc cacacgcgtc tccatcaccg gcgtttccag cccctgcaag
     1021 ccaataccta tcggataaac ctgcacgata ttttctcccg gcggataata ataaaggcgc
     1081 agctctgcaa ggttaacgat aatcccttga cgcggcgcat caggtaataa cagttgcgaa
     1141 ggaatagtta tcgtcgtacc gggttttggc accggggcga tagtgttatt ggcttcaagg
     1201 atcaacattg ccgcagtatc aaaacgtcgg gcgatggcct gaaggttttt atccccttcc
     1261 tgcaccgtat acgtttgatt ttgcccaacc agtcggcttc cggttggtgg tagcggataa
     1321 tcaaccgccc agccagcctg gatggcgcta aaagcgccga taagcgtgag tgtaagcaaa
     1381 gacgcgcgtt tcattgtaaa cctcctgtat ttgccggaga ctcacgctga aacgtcggat
     1441 ggcgcttatg ttcacctgaa accgaaacac tcctgtgcag gtcagtgtaa acattgacca
     1501 tccggcaatg tgagccaacc ggatgaaagc tgtcccttta gtttagctaa gtgcagcggc
     1561 tttggcgcga attgcgcgaa tcatcgcttc cagaccttgt gaacgagatg gggtgagatg
     1621 ttgggtgagc gccatttttt caaaccacgg acgcacatcg aaattgacaa tatcctgcgg
     1681 cgtcatctga tcgtagagaa taaagacgac cgcaataagc cctttcacaa tcgccgcatc
     1741 gctgtcgccc tgcaattcaa taattccctg cgcattctgg cgcatgacaa tccacacctg
     1801 actctggcag ccctgaatgc tattttgtgg gcttctgtct tcgtcgcgta attctggcag
     1861 acgctggccc agctcaataa tgtagagata tttctcttcc cagttggcgc agcgtaaaaa
     1921 attacgcagc aatttttctt tatccggcaa tacagccata gtgcctccct gttatcccag
     1981 cagacggtga atacgttgca gaccggtcac cagacgatcc acttcttcat gggtgttata
     2041 catggccagc gacgcccgac acatagcagg gacgttataa taggccatta atggcatagc
     2101 gcagtgatgt ccggtacgca cagcaatgcc gtaattatcg agaaaactgc caacatcata
     2161 ggcgtggtgt ttaccgagat taaaagcaat aacgccaagc ctgttttgtg ggccatagag
     2221 agtgagatcc ggtacagatt ccagctgtga tagcgcgtaa tgcatcagat tctgttcata
     2281 ctcggctatg ttattaagcc ccagcgccga aacataatcc agcgccgcgc caagaccaat
     2341 gatgcccccg gtattgggtg taccggcttc aaaccgccac ggtgctttgg tccaggtagt
     2401 gccttcactc aggctgacgg tggcgatcat agaaccgccc ccttcccacg gcggcatctc
     2461 ctgcaacaag gcttctttca cataaagaat gccaatcccg gtggggccat acagtttatg
     2521 cccggagaac acgtaaaagt cgcaatccag cgcctgaaca tccaccgaat gatgcatcac
     2581 cgcctgagcg ccatccacca gcacttttgc gccatgctgg tgcgcaagcg tgatcattcc
     2641 cgccagtgga ttttctgtgc caagcacgtt ggagacatga gtaattgcca gcaggcgagt
     2701 tttctcatca aacagcgtag gcagcgtctc cagttgcagc gtaccgtcgg ggttgagagg
     2761 gatcacacgc agctctgcgc caacgcgtgc gcaaagcatc tgccagggga caatgttagc
     2821 gtggtgctcc atctgactga tgatgatgtt atcgcccgcc cgcacattgc tgttgcccca
     2881 gctattggcg accagattga tcccttccgt cgtgccgcgg acgaacacca gctcttccgc
     2941 cgaacgggca ttaataaaca gcgatgcccg cttgcgcacg ttctccattt tctcggtcgc
     3001 ctgggcgctt aaggtatgaa taccgcgatg caccgccgcg tagccatgac gataaaactc
     3061 ggcctcggcg tcaatcacct ggctcggttt ctgtgcactg gcggcgctgt cgagataagc
     3121 cagcggcaaa ccgtttacct cacgcgaaag caccggaaag tcggcccgca ctttgtcgac
     3181 ggaaaaagtc atcttgcacc tcctggcagc cgttgaccga ttcgggccag cacctgctgt
     3241 ttaagcccct catcacgcag tgcttccgtc agttcggcag caaaggcgta aatgatcatc
     3301 tgctgggcat cctgctgatt gatcccgcgc gagcgcagat agaacatctg ttcatcatca
     3361 atacgcccca ccgtcgcgcc gtggctgcat ttcacatcat ctgcatagat ttccagctgc
     3421 ggtttcgtat ccacttctgc cagttttccc atcagcagat tattgttggt catctgacca
     3481 tccgttttga tggcgtgctg cgcgacgttg atcaaaccgt taaatacagc gcggcctttg
     3541 tcgctgacga tagttttgtg caactgtcgg ctgttacaaa aacctttatt gtgttccagc
     3601 caggtacggg tatcacacac ctcgtttttc accggcatcg ccaggctatt gatccgcaac
     3661 atgctgtttt cgccattgag ttgcgtactg gtgttgtgtc gtaacactgc gccacccagc
     3721 aggaaactgt ggctaaatgc ggtggcatcc tcagccagca acaaatcgtt atgagcaaag
     3781 tggtgactga gcgggttttc aaacgccagc ttgatatgct gcaagtgggc gttcgctgcg
     3841 acattgatag tgaaccgtgc cccggtaaaa tgacgagcat cattaaggct gacaaaatgt
     3901 tcgatcaccg ttgcttcggc accttccgcc agatccagat gatgccggta atgggcagtg
     3961 ttcacctctt cacctgccac gccctgggtg atatgcatta acagcaatgg ctttgccggc
     4021 cgttgaccgc gcttcacggc gatatgcgtc acgctttgtg ccaggctttc cgtcaaatgc
     4081 agaaacactt ccgcctgaat agcgtcgggt aacccctgac ggtcgtcgtt aatgctcact
     4141 tcatatccgc tgccttcaat tgcatcgctc agcgcgggca cgtaacgtcc atcgacaaac
     4201 accagccgca cggcgtctaa cgttaacgct aaggcatcac gctgctgtgg ggatatctcc
     4261 cccgcaatgc tgacaaactg gctattggtc agcccttcca gcggcgtata tttccagttt
     4321 tcatgtttac gtgtcggcag tccggtacgc agcaattgtt gtaaatgctg ctgtgcttgc
     4381 ggggagcgtt ttgtcccttc agcttcaaac aagtgatgcc actgttgcag cgcgttactg
     4441 ctgttcggta agccagccat aaccctgctc ctccagttgt ttgaccaacg tgaaatcgcc
     4501 ggatttcaca attcgtccct gatatagcac atggacgtaa tcaggcttga tgtagtcgag
     4561 aatgcgttgg tagtgcgtaa caatgatgaa tgagcgcttg ccatcacgca gcgagttcac
     4621 gccatcggcg accactttta atgcgtcaat atccagcccg gagtccgact catcaagaat
     4681 gcataactcc ggttccagca ccgccatttg caaaatatcg ttgcgctttt tctcgccgcc
     4741 ggaaaaacca acgtttaccg aacgggttaa taaatcttcc ggcatcttca ggagagcgat
     4801 tttctcttcc atcaaatcct gaaaatcaaa gcggtcgagc gtttcctggc cgcgatagct
     4861 gcgcactgca ttaagtgccg tttgcaggaa aaactggtta ctgacgcctg gaatctccac
     4921 cggatactgg aaggccataa agatgccttc gcctgcacga tcttccggcg acagtgcaag
     4981 caaatctttg cctttgaact caaccgtgcc gcccgtcact tcataatctt cgcgcccggc
     5041 aagcgttgcc gataaggtac ttttgcccga accgtttggc cccataatgg cgtgaacttc
     5101 gccgggacga acggcgaggc ttaatccacg caggatagct ttatcttcca cgttgacgtg
     5161 taaatcttta atacttaaca tgtttattcc ttatccgacg ctgtgttcaa gactgatggc
     5221 gaggagtttt tgtgcttcaa cggcaaattc cagcggcagt tctgagaaca cgtctttgca
     5281 gaaaccgtta acaatcatcg agatggcgtc ttcttcgctg atcccgcgtt gcaggcagta
     5341 aaacagttga tcttcaccaa tacgtgatgt cgttgcctcg tgctccagtt gtgcgctgtt
     5401 attgcgacat tcgacatacg ggaaggtatg cgccccacaa ttagcgccaa tcagcattga
     5461 gtcgcactga gtgaaattgc gcgcattggt tgccgtcggc atgattttca ctaagccgcg
     5521 ataactgttc tgactatgtc cggcagagat ccctttcgag ataatggtcg atttggtgtt
     5581 tttaccgatg tggatcatct tggtgccggt atccgcttgc tgatgaccgc tggtcagcgc
     5641 cactgagtaa aactcaccaa tggagttatc gccgcgcaaa atgcagctgg gatatttcca
     5701 cgtaatcgcg gacccggttt ctgattgcgt ccatgacatt ttgctgtttt cgccttcgca
     5761 caaagcacgc ttggtgacga agttgagaat accgccggtg ttgttatcgc cagggaacca
     5821 gttttgtacc gtggaatatt tcacctcggc gtttttatgg atgatgactt ccaccaccgc
     5881 cgcgtgtaac tgatagctgt cacgcaccgg agcggaacag ccttcaatgt agctgacgta
     5941 gctgtcttcg tcggccacca gaatggtgcg ctcaaactgc ccggtttttt cagcgttaat
     6001 gcgaaaatag gtggaaagtt ccatcgggca gcgcacgcct ttaggcacat aaataaacgt
     6061 accgtcagag gctaccgccg cattaagcgc ggcaaagaag ttgtcattcc ccggcaccac
     6121 ggtgccgaga tatttacgca ccagttccgg gtgatcgtgg atcgcctcac caaaggaaca
     6181 gaaaataatt ccctgctccg ccagtttttc gcgataagta gtggcaaccg aaactgagtc
     6241 gaaaatggca tccaccgcca cctctttgcc ttcccgcacg ggaacgccca actgctcaaa
     6301 cgccgcctcc acctctttac ttaaaaaggc gttcgcgcca gtttgctgca ccgcgccagg
     6361 ttcagacgcg caagtgtcgt cacaattacc gcacgatggt gctgagtagt agctgtaatc
     6421 ctgataattc agcttgtcgt agtgcgcttt caaccagtgc ggttcttcca tctccagcca
     6481 tgcgcgatag gcgtttagac gaaactccag catccactcc ggctcattac gcttcgccga
     6541 aattgcgcgc accacctctt cgtttatccc ctttgccagc tcatcggtgg ctaactgggt
     6601 gaagaatcct tctttataat tcagcgggcc gccggtccag gttttgacat cgtcagttgc
     6661 ttcagtatta cgagacatag taccgcctat accccaaagc tttcgccaca gccacattca
     6721 ttctgggctt tagggttgtg aaatttgaat atctgattaa gtccttcacg aacgaaatcg
     6781 acttccgtgc catcaataaa cggcatcgct tgcagcggga caaacagctt cgcgccgtcg
     6841 tgttcaaaca gcagatcgtc tttgtccggc tcgctaacac tgtcgagcac atagccaaag
     6901 cccgcgcagc ccgtttgctt cacgcctaag cgcacgccga ccatacccgg ctgctttgcc
     6961 accagctcac ggatgtgtat cgccgctgcg ggtgtcagcg ttaagccttg ccaggcgaaa
     7021 tcttgtgggt taaaggttcc tgaatgcatg tccatcgatt tacctcactt catcgctttc
     7081 agcgtataac agcatgttag tgataatgat tatcagttca acccagcaaa cgcaggggct
     7141 ttaccgtaaa acatgctttt tgcctgcttt taataagcat agaccctgat gtgtgggtta
     7201 acaggcacgc taagaattag gtatctcatt gttagataat ggttattatc taaggagcat
     7261 taaggctgtg atgaaaaaga aaaagttgta ttgaaaatga ctatttaaga gataggtaaa
     7321 aaagtgcagc gttcagaaat aagaaaaccc ttaagtctgt gcgacacagg cttaagggtt
     7381 tctaccccat ccggcgctta tctccggcac tctcagtggc ttagctcttg aaggggcgat
     7441 aagaataatc tcataaagct aacccgccgt tttaacacaa attgcgatta gtattatttt
     7501 tgaacaatat caggcgggta gataagcagt attaagaagg tcatcgaacc tggacggagg
     7561 ttaatccagg tcgatttggc gaacttgcgg cattaagtca ggatcaatgc acgcccagac
     7621 gccaggcaaa gtagatttct tcttttaatt cagcagaaga gagagtaagc aggtcagcaa
     7681 attcaagttc tagttgtttc agacggttga gatattgggc aggtgaaaga ttgctctggt
     7741 cacggcgtaa aaattcaatg gccagctggg tgggatcaag ttgagtagac atagcatcct
     7801 cgcttttaga caagacctgc acagtatacc accgtttact gtgcagataa tgaccaaaag
     7861 caatatgcgt cacacttttc tggtgacaac gtcacaaaat ggcggtcgtc aatcgtgacg
     7921 aacagcacaa acgccctttc tcatcgaaga tttcaatctg ccagacctgg tgacgcgaac
     7981 cgagatgcaa cggtttgcat acgccgcgca cccgcccttc tcgtgccgag cggacgtggt
     8041 tagcattgat ttccagacca accacttttt gctcaccttc ggtacataaa taaccggcaa
     8101 cggaaccgat actttcggcc agtaccacgg atgcacctcc atgtagcaac ccgaaaggct
     8161 gctttgtccg cgagtctact ggcattgtcg cttcaagggt gtcatcacca atatgttcaa
     8221 agcgaatatc cagtaacccc accatgtttc cttcacccat ggcattcagt gcttccaggg
     8281 tgatttttcg tttccatatc atttaataat ctccagtaaa gcctgcacag gatggcgtac
     8341 ccccgtgcct tcaacccgtt tgacctgact acggcaggaa tatccggtcg ccagacagcg
     8401 gttacgcggc agtcgctgca tagcctgatg ccaggataac tcatagatcc cgagcgaatt
     8461 tttatggttt ttcgcttcat gtccgtaagt ccctgccatg ccgcagcaac ccacgctgac
     8521 attttccagt ttcgcgccaa aacgggcaaa tatcgcggcc catagtgctg gcgcacccgg
     8581 caaggcggta acttcggtac agtgaccaaa gaaataccat gattcaccgc tgactgtagc
     8641 cactggctgt gactcaagtg cgcttgccag ccattcattc gccagtaaga cgttaaactc
     8701 gccacgttcc tcgcccagcg ccagtttata ttcatcgcga taacaaagta ccagcgccgg
     8761 atcgacgccc accattggca tacccagctt cgccatacgg ttgaggaaat ccgccgtctt
     8821 tttcgccgtc ttcgcaaaac gattaagaaa acctttaata tgctgggctt tgccatttgg
     8881 cgaaaatggc agtaacacag gctggaaacc taatttttcg accagacgga caaaatccgc
     8941 caccacttgc gcatcgtaat agctggtaaa ggggtcctgc accaccaaca ctgtgcgcgc
     9001 tttctgctct gcattgagag attcaagctg ttccagcgtc atgtttgccg agcgatgccc
     9061 caccatttgt tgttgtagcg aggggaccga cagcagcggc aaatcaacca tgccgatatg
     9121 tttttccgag agtttgcgca ccagcggctg gttaatgaag aagttaaagg ttttcggcgc
     9181 gcgtgccatc agcggcgcgt agctctcgac cgtagcgacg aggtggtcgc gcagcgggcg
     9241 taaataacgg gtgtgataga gctgcagaaa acgagagcga aactccggca catcaatttt
     9301 gatggggcac tgggtcgaac acgctttaca ggccagacag cccgacatcg cctctttgac
     9361 ttcgtgtgag aagtcatatt cgcctttatt cgcatgccag ctattgcgcg tgcgggcaat
     9421 taacgtccgc aaactgacgc cggattcagg cagttcttgt tccagtttga gtggatcaac
     9481 gccgcgatcc gccaacaaac gcagccattc acgcaccagc gttgcgcgcc ctttcggtga
     9541 atgaatccgg ttctgggtga tcttcatcga cggacacatc ggactacggg catcaaagtt
     9601 gaagcataaa ccgttgccgt tacactccat cgcaccgcgc cactgctggc gtaccgcaat
     9661 ggggatctgc cgatcgaatg taccgcgctt caccgcgtcc actttcatca tcggcgcatc
     9721 gagaccttct ggcgggcaaa tcttccctgg gttgagtcgg ttatgcgggt caaatgccgc
     9781 tttcactttg cgcagttctg caaaaagttc ctcaccgaaa aacgccgggc tgtattcagc
     9841 gcgaaaacct ttgccgtgct cgccccacaa caaaccaccg tatttcgcag tcagcgccac
     9901 cacgtcatca gagatttgct tcatcaaaat ctcttgttgc ggatcgcaca tatccagtgc
     9961 cggacggacg tgcaagacac ctgcgtcgac gtgaccgaac ataccgtagc ttaagccgtg
    10021 gctgtcgagc agcgcgcgaa attcagcaat ataatcggcc agatgttccg gcggtacgca
    10081 ggtatcctca gcaaacggaa ttggcttagc ggcacctttg gcattgccaa gcagaccaac
    10141 ggcttttttg cgcattgcat agatacgttc aacccccgcc agctcacggc acatctgcca
    10201 gccgatgaca cctgcttgat gactggcgat cagctcatcc aaccgcgcac agagtgcatt
    10261 tacccgctcg tcaatcagcg cctcatcatc accagcaaat tccacaatgt tcagcccgag
    10321 catctctttg tcaggcacat cggtaatcaa ctcgctgacg gaatgccaga caatatcttc
    10381 ccgcgccaga ttcagcactt ttgagtccac cgtctctacc gaaagcgccc gcgcctcaac
    10441 cataaacggc gcgttacgca gcgcagagtc aaaagagtca tatttgacat tcaccagacg
    10501 gcgcacttta ggcaagcgcg taatatccag ccgcgcttcg gtaataaagg ccagcgtccc
    10561 ttctgaaccc gtcagaatgc gcgtcaggtc gaactcggtc atctcatcgt taaagacatg
    10621 acgcagatcg taaccggtaa gaaagcggtt aagtttgggg aagttgtcga tgattaactg
    10681 gcgttgctga cggcaacgtt gataaaccgt gttataaatt cgcccgattg tggtattgga
    10741 tttacccagc gtttccgcca gctcgacggg taaaggttgc gtatcgagaa tatcgccccc
    10801 caacagtacc gcacgtaggc caagtacgtg atctgacgtt ttgccataga ccagcgatcc
    10861 ctgaccggat gcatcggtat tgatcatccc gccgagcgtt gcccggttgc tggtcgaaag
    10921 ttctggtgca aaaaagtagc cgaacggttt caggtactga ttgagttggt cttttatcac
    10981 cccggcctca acgcgcaccc agccctcttc agggttaatt tcgatgatgc ggttcatatg
    11041 gcgggacata tcaacaataa tcccctggtt gagcgcctga ccgttagtgc cggtgccgcc
    11101 gccgcggggg gtaaagatca gcgatgaata gcgttcctgc gcggcaagac gggcgatcag
    11161 cgccacatct gcggttgaac gcggaaatac caccgcatcg gggagaagtt ggtaaatact
    11221 gttgtcggtc gacattgtca gacgatcggc ataacttgtc gccgtatcgc cggtaaaacc
    11281 ttgttgctcc agctcttgca aaaaattaag caccagttga acgacgccgg gtgcctggga
    11341 aatctgtgga atcattatat tgaccctttc ctgcggtctg tgatgtaggt cgatacactg
    11401 ttctttcagg cggctgcaat agcgcacaga aaggtgatgt ttatttactc tatggatttc
    11461 gagttgcagg aaggcggcaa gcgagtgaac tccaggagct tacaacagta agtaactggg
    11521 gtgaacgaac gtagccgcag catatgcaac ttgaaatacg acgagtaaat cgtttgcgtg
    11581 ttgcctgagt tgttgtacca catttttttc taacacgccc atcagaatta agggcagaat
    11641 cggcctgtta aaaaccgctg aaattgctca tcattatgca ggtgagtttc gcgtgttcac
    11701 gtcgcgtcga cgatttgacg cacaaaaaag gtgaaaagta gttatggtaa atgttcgtca
    11761 gcccagggat gtcgcacaaa ttctgctttc ggtgctgttt ttagccatca tgattgtggc
    11821 atgtctgtgg attgttcaac cctttattct cggctttgca tgggccggta cggtggttat
    11881 cgccacctgg ccggtattgt tacgtttgca aaagatcatg tttggccgcc gctctctcgc
    11941 cgttctggtg atgacgctgt tattagtgat ggtgtttatc atccctattg ctttgctggt
    12001 taacagtatc gtcgacggca gcggcccgct aattaaagcc atttccagcg gtgacatgac
    12061 gttacccgat ctggcgtggc ttaataccat tccggtgatt ggcgcgaagc tgtatgcagg
    12121 ctggcacaac ttgctggata tgggggggac ggcgatcatg gcgaaagtcc gcccttatat
    12181 tggcaccacc accacctggt tcgttgggca ggcggcgcat atcgggcgct ttatggtgca
    12241 ttgtgcgctg atgcttctct tcagtgccct gctgtactgg cgcggtgaac aggttgcaca
    12301 aggcattcgc cattttgcaa cccgtctggc aggcgttcgc ggagatgccg ccgtactgct
    12361 ggcggcacag gctatccgcg cggtggcgct gggtgtggtg gtgacggcgt tagtacaggc
    12421 agtgcttggc ggtatcggcc tcgccgtttc cggcgtacct tatgcaactt tgctaacggt
    12481 gttaatgatc ctctcctgcc ttgtccagct tggcccgttg ccggtactga ttccggcgat
    12541 tatctggctc tactggaccg gcgataccac ctggggaacg gtattgttag tgtggagcgg
    12601 tgtggttggc acgctggata acgtcatccg cccaatgtta attcgcatgg gtgccgattt
    12661 accgctgatc ctgatcctct ctggcgttat tggtggtttg attgctttcg ggatgatcgg
    12721 tctgtttatt ggtccggttc tgttagccgt ttcctggcgt ctgtttgccg cgtgggtgga
    12781 agaagtcccg ccgccgacgg accaaccgga agaaattctc gaagaacttg gcgaaatcga
    12841 gaaaccgaat aagtaatttc tcatcaggcg gctccgccgc ctgattgtta accaccgcta
    12901 attgatattt gcttgctctt ccccatattt tcctgcttac cgccattcag ctggtagtac
    12961 ctgtcgcaaa ttctttacag tttttaaact aatgagacga atctgatcga cgcaaaaagt
    13021 ccgtatgcct actattagct cacggttata aatcaacata ttgatttata agcatggaaa
    13081 tcccctgagt gaaacaacga attgctgtgt gtagtctttg cccatctccc acgatgggct
    13141 tttttttaac atttttccgc atcgctacct cgcccctcac tctttccaat aatgttgcac
    13201 aagatactgt gactctcttc aagccgtcag caggctgata atgttattta tattttattg
    13261 tttatttcat gcagccatta cacgatgaac gcttatgaac tccaggcact ccgccatatt
    13321 tttgccatga ctattgatga atgcgcgacc tggattgccc agaccggtga cagtgaaagc
    13381 tggcgtcaat gggaaaatgg caagtgtgcc attcctgatt gtgtagtcga gcaactgttg
    13441 gctatgcgtc agcaaagaaa aaaacatctt catgcgatca tagagaaaat aaataaccgc
    13501 atcggcaaca acaccatgcg ttttttcccc gacttaaccg cgtttcaaca agtctaccct
    13561 gatggcaatt ttattgactg gaagatctat caatcggtag ccgctgaatt atatgcacac
    13621 gatctggagc ggctttgtta atttctccac aaaaaggaat tgtcgttgtt acaacaataa
    13681 tgaacggata ctgacacaac atcgctacac tttttaaagc acctttgcta agtagaacct
    13741 atgaaaaatc cctattaccc taccgcactt gggttgtatt ttaattacct ggtgcatggt
    13801 atgggcgtca ttttgatgag cctgaatatg gcctcgctgg agacactttg gcagactaat
    13861 gccgcgggtg tctcgatagt tatctcatcg ctgggcattg gtcgattaag tgtcttgctt
    13921 tttgcaggat tattatccga tcgctttggt cgccgccctt ttatcatgct cgggatgtgc
    13981 tgctatatgg ccttcttttt tggcatcctg cagaccaata acatcattat cgcttatgtt
    14041 tttggctttc tggcgggaat ggcaaacagt tttctcgatg caggcactta tcccagtttg
    14101 atggaagctt ttccacgctc acctgggaca gccaatattt taattaaagc atttgtttcc
    14161 agcggacaat ttttattacc gctaatcatt agcctgttag tgtgggctga actgtggttc
    14221 ggttggtcct ttatgattgc tgcaggcatt atgtttatta acgctctgtt tttataccgt
    14281 tgtacgttcc caccccatcc gggtcgtcgc ttacctgtca taaagaaaac caccagctct
    14341 acggaacatc gctgttcaat tatcgattta gccagttata ccttatatgg ctatatctca
    14401 atggcaacgt tttatctggt tagccagtgg ctggcacagt acggacaatt tgttgcaggc
    14461 atgtcataca ctatgtcgat caaactactc agtatctaca ccgtgggttc gctgctttgt
    14521 gtatttatta ccgctccact cattcgtaat accgttcgcc caacaacatt actgatgctg
    14581 tacaccttta tctcatttat tgctctgttt accgtctgcc tgcatcccac attttatgtg
    14641 gtgataatat ttgcttttgt cattggtttt acctctgctg gaggtgttgt gcaaattggc
    14701 ctgacgttaa tggctgaacg tttcccttac gctaaaggta aagctacagg gatctattac
    14761 agtgcgggca gtattgcgac ctttactatt ccgttgatta cggctcatct gtcccaaaga
    14821 agtattgccg atattatgtg gttcgatacc gccatcgctg ccatcggttt tttactggca
    14881 ctgtttatcg gcttacgcag ccgcaaaaaa acgcggcatc actcgctaaa ggaaaatgtc
    14941 gctccgggtg ggtaatgcaa tattcttttc aggtcatgca agatcttacg gataaataac
    15001 tctttctgcg ctaactaagg aaaatcgcga tcaaaaacaa actatgacat gcaatattcc
    15061 tggaaacata aactttatgc catgtaccca gggaaaatca tcttcagtat agtaattatg
    15121 taaaccgtcg gagaacaata cgtacggtaa cgaaattatc tttcagcaag gagctgtgaa
    15181 aatgtctcaa aataaggctt tcagcacgcc atttatcctg gctgttcttt gtatttactt
    15241 cagctacttc ctgcacggca ttagtgttat tacgcttgcc caaaatatgt catctctggc
    15301 ggaaaagttt tccactgaca acgcgggcat tgcctactta atttccggta tcggtttggg
    15361 gcgattgatc agtattttat tcttcggtgt gatctccgat aagtttggtc gtcgggcggt
    15421 gatattaatg gcagtaataa tgtatctgct attcttcttt ggtattcccg cttgcccgaa
    15481 tttaactctc gcctacggtc tggcagtgtg cgtaggtatc gctaactcag cgctggatac
    15541 gggtggctac cccgcgctca tggaatgctt tccgaaagcc tctggttcgg cggtcatact
    15601 ggttaaagcg atggtgtcat ttgggcaaat gttctaccca atgctggtga gctatatgtt
    15661 gctcaataat atctggtacg gctatgggct gattattccg ggtattctat ttgtactgat
    15721 cacgctgatg ctgttgaaaa gcaaattccc cagccagttg gtggacgcca gcgtaactaa
    15781 tgaattaccg caaatgaaca gcaaaccgtt agtctggctg gaaggtgttt catcggtact
    15841 gttcggtgta gccgcattct cgacctttta tgtgattgtg gtgtggatgc ccaaatatgc
    15901 gatggctttt gctggtatgt cagaagctga ggcattaaaa accatctctt attacagtat
    15961 gggctcgttg gtctgtgtct ttatttttgc cgcactactg aaaaaaatgg tccggcccat
    16021 ctgggctaat gtatttaact ctgcactggc aacaataaca gcagccatta tctacctgta
    16081 cccttctcca ctggtgtgta atgccggcgc ctttgttatc ggtttctcag cagctggcgg
    16141 cattttacag ctcggtgttt cggtcatgtc agagtttttc ccaaaaagca aagccaaagt
    16201 caccagtatt tatatgatga tgggtggact ggctaacttt gttattccgc tgattaccgg
    16261 ttatctgtcg aacatcggcc tgcaatatat cattgttctc gattttactt tcgcgctgct
    16321 ggccctgatt accgcaatta ttgtttttat ccgctattac cgcgttttta ttattcctga
    16381 gaatgatgtg cggtttggcg agcgcaaatt tagcacccgg ttaaacacaa ttaagcatag
    16441 aggttaaagg agttaattat ggatgttacc gcaaaatacg aattgattgg gttgatggct
    16501 tatcctatcc gccacagttt atcgcctgaa atgcagaata aagccttaga aaaagcggga
    16561 ttgccattta cctatatggc cttcgaagtg gataacgata gctttcccgg agcaattgaa
    16621 ggattaaaag ctctaaaaat gcgcggaact ggtgtatcaa tgccgaacaa acaactggcg
    16681 tgtgaatatg ttgatgaatt aacgccagca gcaaaactgg tgggtgccat caacaccatc
    16741 gttaatgatg atggctacct gcgcggctat aacaccgacg gcacgggcca tattcgcgcc
    16801 attaaagaga gcggttttga tatcaaaggc aaaacaatgg tgctgttagg ggccggaggt
    16861 gcctcaacgg cgattggcgc gcagggggca attgaaggtt taaaagaaat taaactcttt
    16921 aaccgtcggg atgagttctt cgataaagcc ctcgccttcg cgcagcgggt taacgaaaac
    16981 accgattgtg tcgtcacggt taccgatctc gccgatcagc aagcctttgc tgaagccctg
    17041 gcttccgccg acattttaac caatggcaca aaagtgggta tgaaacccct tgagaatgaa
    17101 tcattggtta atgatatcag tctgttacat ccgggactgc tggtcactga atgcgtgtat
    17161 aacccgcata tgacgaagtt attgcagcag gcgcaacaag ctggttgcaa aacgattgat
    17221 ggatacggca tgttgttgtg gcaaggggct gaacagttca cgttatggac tggcaaagat
    17281 ttccctctgg aatatgttaa acaggtcatg gggttcggtg cctgacaggc tgaccgcgtg
    17341 cagaaagggt aaaaaatgaa aaccgtaact gtaaaagatc tcgtcattgg tgcgggcgca
    17401 cctaaaatca tcgtctcgct gatggcgaaa gatatcgccc gcgtgaaatc cgaagctctc
    17461 gcctatcgtg aagcggactt tgatattctg gaatggcgtg tggaccactt tgccgacctc
    17521 tccaatgtgg agtctgtcat ggcggcagca aaaattctcc gtgaaaccat gccagaaaaa
    17581 ccgctgctgt ttaccttccg cagtgccaaa gaaggcggcg agcaggcgat ttccaccgag
    17641 gcttatattg cactcaatcg tgcagccatc gacagcggcc tggttgaaat gatcgatctg
    17701 gagttattta ccggcgatga tcaggttaaa gaaaccgtcg cctacgccca cgcgcatgat
    17761 gtgaaagttg tcatgtccaa ccatgacttc cataaaacgc cggaagccga agaaatcatt
    17821 gcccgtctgc gcaaaatgca atccttcgac gccgatattc ctaagattgc gctgatgccg
    17881 caaagtacca gcgatgtgct gacgttgctt gccgcgaccc tggagatgca ggagcagtat
    17941 gccgatcgtc caatcatcac gatgtcaatg gcaaaaactg gcgtaatttc tcgtctggct
    18001 ggtgaagtat ttggctcggc ggcaactttt ggtgcggtaa aaaaagcctc tgcgccaggg
    18061 caaatctcgg taaatgattt gcgcacagta ttaactattt tacatcaggc ataagcaata
    18121 atatttcggc gggaatatcc tcccccgccg aactaaaaaa aatatattca accgtattta
    18181 ataaaaatat ttcgtgagtc tctgtgcgct aattatccat ctggcgtagg gaaaatcaca
    18241 cctgaatcag gaattaacaa tgaaacctgt aaaaccacct cgtattaatg gacgagtgcc
    18301 ggtcctgtcg gcacaggaag cggtgaatta tattcccgac gaagcaacac tttgtgtgtt
    18361 aggcgctggc ggcggtattc tggaagccac cacgttaatt actgctcttg ctgataaata
    18421 taaacagact caaacgccac gtaatttatc gattattagt ccaacagggc ttggcgatcg
    18481 cgccgaccgc ggtattagtc cgctggcgca agaaggtctg gtgaaatggg cattatgcgg
    18541 tcactgggga caatcgccgc gtatttctga tctcgcagaa caaaataaaa ttattgctta
    18601 taactatcca caaggtgtac ttacacaaac cttacgcgcc gccgcagccc accagcctgg
    18661 tattattagt gatattggca tcgggacatt tgtcgatcca cgccagcaag gcggcaaact
    18721 gaatgaagtc actaaagaag acctgattaa actggtcgag tttgataaca aagaatatct
    18781 ctattacaaa gcgattgcgc cagatatcgc cttcattcgc gctaccacct gcgacagcga
    18841 aggctacgcc acttttgaag atgaggtgat gtatctcgac gcattggtta ttgcccaggc
    18901 ggtgcacaat aacggcggta ttgtgatgat gcaggtgcag aaaatggtta agaaagccac
    18961 gctgcatcct aaatctgtcc gtattccggg ttatctggtg gatattgtgg tggtcgatcc
    19021 ggatcaaacc caactgtatg gcggtgcacc ggttaaccgc tttatttctg gtgacttcac
    19081 ccttgatgac agtaccaaac ttagcctgcc cctaaaccaa cgtaaattag ttgcgcggcg
    19141 cgcattattc gaaatgcgta aaggcgcggt ggggaatgtc ggcgttggta ttgctgacgg
    19201 cattggcctg gtcgcccgag aagaaggttg tgctgatgac tttattctga cggttgaaac
    19261 aggtccgatt ggcggaatta cttcacaggg gatcgccttt ggcgcgaacg tgaatacccg
    19321 tgccattctg gatatgacgt cccagtttga tttttatcac ggtggcggtc tggatgtttg
    19381 ttatttgagt tttgctgaag tcgaccagca cggtaacgtc ggcgtacata aattcaatgg
    19441 taaaatcatg ggcaccggtg gatttattga tatcagtgcc acttcgaaga aaatcatttt
    19501 ctgcggcaca ttaactgcgg gcagtttaaa aacagaaatt accgacggca aattaaatat
    19561 cgtccaggaa ggacgggtga agaaatttat tcgggaacta ccggaaatta ctttcagcgg
    19621 aaaaatcgct ctcgagcgag ggctggatgt tcgttatatc actgagcgcg ccgtattcac
    19681 gctgaaagaa gacggcctgc atttaatcga aatcgcccct ggcgtcgatt tacaaaaaga
    19741 tattctcgac aaaatggatt tccccccagt gatttcgcca gaactcaaac tgatggacga
    19801 aagattattt atcgatgcgg cgatgggttt tgtcctgcct gaagcggctc attaacagga
    19861 gtatcacgat ggatttttct ttaactgaag aacaagaact gctgctggcc agtattcgcg
    19921 aactgattac gactaacttt ccggaagagt atttccgcac ctgcgatcaa aacgggacat
    19981 atccgcgtga gtttatgcgg gcactggcgg ataacggtat ttccatgctt ggcgtgccgg
    20041 aagaatttgg tggtatccct gctgattacg tcacccaaat gctggcgctg atggaagtgt
    20101 caaaatgcgg tgctccggca tttttaatta ccaacggtca atgtattcac agtatgcgcc
    20161 gtttcggttc tgcagagcag ctacgtaaaa cggcagagag cacccaggaa acgggtgatc
    20221 ccgcctatgc cctggcgttg acggaaccag gtgctggctc agataacaac agcgccacca
    20281 ccatttacac acgtaaaaac ggcaaggttt acatcaacgg acagaaaacc tttattaccg
    20341 gtgcgaaaga gtacccatat atgctggtgt tggcgcgcga tcctgaaccg aaagatccca
    20401 aaaaagcctt caccctgtgg tgggtcgact ccagtaagcc cggcattaag attaacccgc
    20461 tgcataaaat cggctggcat atgctcagca cctgcgaagt ctatctcgac aacgtggaag
    20521 ttgaagagag cgacatggtg ggcgaagaag gaatgggttt cctcaatgtg atgtacaact
    20581 ttgagatgga gcgcctgatc aacgccgcgc gcagcaccgg ctttgccgag tgtgcctttg
    20641 aagatgccgc tcgctatgcc aaccagcgta tcgcttttgg taaacccatt gggcataacc
    20701 agatgatcca ggaaaaactg gcgctgatgg cgattaagat cgacaacatg cgcaacatgg
    20761 tgctgaaagt ggcatggcaa gccgatcagc atcagtcact gcgcaccagc gcggcgctgg
    20821 caaaactgta ttgcgcacgt accgcaatgg aagtcattga tgatgcgatt caaatcatgg
    20881 gcggtctggg ctataccgat gaggcgcgcg tctcccgctt ctggcgtgat gtccgttgtg
    20941 aacgtatcgg cggcggtaca gacgaaatta tgatttacgt agcaggtcgg cagatcctga
    21001 aagactatca gaacaaataa tctgcaggcg gcgcagcttc ttaacaaact gcgccgccag
    21061 atttacccgg caagacttac cggttgagga aattcagata agatttcttg ttccgcaaaa
    21121 caatttttaa attgcctgcg gtaatcgctg ggcgaacaac cgacatggcg caaaaacagt
    21181 ttggcaaagt gatcgacatt ttcataaccc acacgccagg agatctctgc ctgtgataat
    21241 tcagtattag tgagtgacca ttttgcttcc gtcatacgcc gttgaataac atagttaata
    21301 ggggaaatac gatactcttt ggtaaattca tggcaaatat aactgacgct ggcacgaaat
    21361 tttttcgata actgttc
//
LOCUS       DAPGOF010000081        21255 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.081, whole genome shotgun sequence.
ACCESSION   DAPGOF010000081 DAPGOF010000000
VERSION     DAPGOF010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.081"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..114
                     /gene="ghoT"
                     /locus_tag="RHV13_002602"
     CDS             <1..114
                     /gene="ghoT"
                     /locus_tag="RHV13_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HDV1242126.1"
                     /translation="IIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF"
     gene            complement(233..1750)
                     /gene="lysS"
                     /locus_tag="RHV13_002603"
     CDS             complement(233..1750)
                     /gene="lysS"
                     /locus_tag="RHV13_002603"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDV1242127.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKNNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(1987..3444)
                     /gene="dtpC"
                     /locus_tag="RHV13_002604"
     CDS             complement(1987..3444)
                     /gene="dtpC"
                     /locus_tag="RHV13_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HDV1242128.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAAGGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(3503..5650)
                     /gene="cadA"
                     /locus_tag="RHV13_002605"
     CDS             complement(3503..5650)
                     /gene="cadA"
                     /locus_tag="RHV13_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HDV1242129.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(5730..7064)
                     /gene="cadB"
                     /locus_tag="RHV13_002606"
     CDS             complement(5730..7064)
                     /gene="cadB"
                     /locus_tag="RHV13_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HDV1242130.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNQTASNAH"
     gene            complement(7430..8968)
                     /gene="cadC"
                     /locus_tag="RHV13_002607"
     CDS             complement(7430..8968)
                     /gene="cadC"
                     /locus_tag="RHV13_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HDV1242131.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSSLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(9585..9660)
                     /locus_tag="RHV13_002608"
     tRNA            complement(9585..9660)
                     /locus_tag="RHV13_002608"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9625..9627),aa:Phe,seq:gaa)
     gene            complement(9767..10342)
                     /locus_tag="RHV13_002609"
     CDS             complement(9767..10342)
                     /locus_tag="RHV13_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDV1242132.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            complement(10379..12076)
                     /gene="dsbD"
                     /locus_tag="RHV13_002610"
     CDS             complement(10379..12076)
                     /gene="dsbD"
                     /locus_tag="RHV13_002610"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HDV1242133.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGAMLTVTYQGCADAGFCYPPETKTVPLSEVVANNAAPQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSSGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            complement(12052..12390)
                     /gene="cutA"
                     /locus_tag="RHV13_002611"
     CDS             complement(12052..12390)
                     /gene="cutA"
                     /locus_tag="RHV13_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HDV1242134.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            complement(12506..13807)
                     /gene="dcuA"
                     /locus_tag="RHV13_002612"
     CDS             complement(12506..13807)
                     /gene="dcuA"
                     /locus_tag="RHV13_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HDV1242135.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            complement(13925..15361)
                     /gene="aspA"
                     /locus_tag="RHV13_002613"
     CDS             complement(13925..15361)
                     /gene="aspA"
                     /locus_tag="RHV13_002613"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HDV1242136.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            15698..16174
                     /gene="fxsA"
                     /locus_tag="RHV13_002614"
     CDS             15698..16174
                     /gene="fxsA"
                     /locus_tag="RHV13_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HDV1242137.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            complement(16190..17446)
                     /gene="yjeH"
                     /locus_tag="RHV13_002615"
     CDS             complement(16190..17446)
                     /gene="yjeH"
                     /locus_tag="RHV13_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HDV1242138.1"
                     /translation="MSGLKQELGLAQGVGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFDWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLTMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            17722..18015
                     /locus_tag="RHV13_002616"
     CDS             17722..18015
                     /locus_tag="RHV13_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDV1242139.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            18059..19705
                     /gene="groL"
                     /locus_tag="RHV13_002617"
     CDS             18059..19705
                     /gene="groL"
                     /locus_tag="RHV13_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDV1242140.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            19843..20196
                     /locus_tag="RHV13_002618"
     CDS             19843..20196
                     /locus_tag="RHV13_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HDV1242141.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(20389..>21255)
                     /locus_tag="RHV13_002619"
     CDS             complement(20389..>21255)
                     /locus_tag="RHV13_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HDV1242142.1"
                     /translation="AISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELRD
                     LLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNTL
                     ELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSLL
                     DFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKTR
                     EETPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHLR
                     VQSTSKTAPQAK"
BASE COUNT         5660 a         5119 c         5206 g         5270 t
ORIGIN      
        1 attatctggt ttatctcaca tgagaaaaca catattcgtt tacttagtgc attcctggtc
       61 ggaataacct ggccaatgag tctgcctgtg gcattacttt tttctctctt ttaggagctg
      121 tagttgagtg gacccgctgg ttatcgtgaa acgaagcgtc tctcctcaaa actggatttg
      181 cggaacgatg aacgaagcag tcgcctgact gcttcgttca ttaaagtgaa atttatttct
      241 gtgggcgcat cgccgggaag agaataacgt cgcgaatagt atggctgtta gtaaacagca
      301 taatcattcg gtcgatacca atacccagac cagcggttgg cggcagacca tattccagcg
      361 cagtcacgta atcttcgtca tagaacatgg cttcgtcgtc acctgcagct ttagcgttaa
      421 cctgttcctg gaaacgttca gcctgatctt ctgcgtcgtt taattcgcta aaaccattac
      481 cgatttcacg accaccgatg aagaattcaa aacggtcggt gatttccggg ttaacatcat
      541 tacggcgtgc cagcggtgac acttctgccg gatattccgt aataaaggtt ggctgaatca
      601 gatgtgcttc tgccacttca tcaaagatct ccgtgacaat acgtcccagc ccccagcttt
      661 tctctaccgt aattccgata gattcagcta atgctttagc agcatcaaaa ttatccaggt
      721 cggccatatc ggtttctgga cgatattttt tgattgcttc gcgcatggtg agtttttcaa
      781 acggtttgcc gaaatcaaac acatgctcgc cataagtgac tttagtggta cccagaacct
      841 cttgtgccag ggtgcggaac agtgactctg tcagttcaat caaatcgtgg taatccgcat
      901 acgccatgta gagttccatc attgtgaact caggattatg gcgaacagaa ataccttcat
      961 tacggaagtt acggttgatt tcgaataccc gttcaaaacc gcctacaacc agacgtttca
     1021 gatacagctc cggcgcgata cgcagataca tatctaaatc cagagcatta tgatgggtaa
     1081 taaacgggcg agcagatgcc ccacctggaa ttacctgcat catcggggtt tctacttcca
     1141 taaagccgcg cgcgaccatg aactggcgga tagcggccag aatttttgaa cggacaacaa
     1201 acgtttgacg ggatttatcg ttagcgatga ggtccagata acgttgacga taacggactt
     1261 cctgatcctg cagaccatgg aatttatctg gtaaaggacg tagtgcttta gtcagcaggc
     1321 gcagctcagt acagtgaatg gaaagctcac ccgtttgcgt cttaaacagc gtaccgcggg
     1381 caccgataat gtcacccaga tcccattttt taaactgatc gttataaaca ccttctggca
     1441 ggctatctcg tgcgacgtac agttgaatac ggccaccgac atcctgcaac gttacaaagg
     1501 aggctttccc catgatacga cgggtcatca ttcggccagc aaccgagact tcaatgttta
     1561 aggattccag ttcctggtta ttcttcgcat caaactcttc gtgcaactgg tcagaggtat
     1621 ggtcgcggcg aaaatcattg ggaaacgcca caccttgctg acgtagtgcc gccagttttt
     1681 cgcggcgatt tctcagttca tcgttaaaat caatagcctc attggctccc cgtgtttctt
     1741 gttcagacat tttggttcct ctaaatccag ctttcaattt agcgtagata aagagacaga
     1801 tcggtacact gtattatctg cctcgactat tagtaactca gtttatctta tcgaaactaa
     1861 cgcttacctc aagttgatat cactaataaa taaccatcaa aatggtttaa atgacttatt
     1921 aatatcgatt tgctagtgtt ataaactgaa agttaattta ttcataacta attgttttta
     1981 cgacagttaa tcgttgctct cctgtatcat attcgtcggc gtgctaaaca ggaaacgggt
     2041 agcaaaggcc aatacgacga tgatcgcgac acaagccaac gtccactctc ccatctgaga
     2101 aaagaatcgc tggtaagctg caattgccat accgctaatt tgcgactctg tcgtctgctg
     2161 tgctactacg cctgccagcc agttggcgac cgcgcctgtt gccagcatat aaatcccggt
     2221 taatacgcca gacattttca gacgcgtgat ttgcgcaatc gccaccgggt caataaagag
     2281 ttcggcaaag cccattagcg ccaacccgga tatcatcacg cccattgacg cttgaccgcc
     2341 agccgctgca tgtcgggcat caaatgccag caacataaag ccacaagcca tcagcagtaa
     2401 gccaaaggca aacttcagcc agacgcgcaa tgttgagttg ccgcggcttt ctggcgatgc
     2461 cagccaggcc agtacaaccc cagcgagcat caccgcaatt gcattcaccg actggaatag
     2521 tgctgtaggt acttcaatat tgaatgcctg acgattcaca aagcgatcga taaacagact
     2581 gatggtactg ccgccctgtt gtgccagtac ccagaacaat gtcccgacaa acatcaacaa
     2641 tacaatttgc caaagagcac ggcggtgttc ggggaatttg atcatcatgc gggcaatgat
     2701 ttgtgcggca atgaggcaaa cgatcgccag caaatatccg gaccagtcgt tctccagcag
     2761 cagggtaaaa aatactgggg ctaaacagag catcaccact aaccagctcc atactggtaa
     2821 ggcaaatttg acgctagtga gcgctttttt atccatacta cgcgtggact ggaaatgacg
     2881 atgaccgctt aagaaaatca acaaaccgat aaacatgccg ccacccgcaa gggcaaagcc
     2941 aacatgccat ccataccact gagcagccag gccgcaggcg atgggggctg cgatagaacc
     3001 gatattgccc gcagcataca gcagcgaaaa accgccatca cgtcgatgat cgttctcgtc
     3061 gtagagctcg ccaagcaaac agctgatgtt tgatttgaat aaaccgtagc cacaaataat
     3121 gattgccagc gccagataca ggctaaaggt tgaatttgta tcaatgccca gcaccacatg
     3181 gccaagggtc attaacagcg cgccggcaat cactgcagtg cggttgccga gcaggcggtc
     3241 ggcaagccag ccgccgagaa taggggtaac gtaaaccaga gaagcatatg cgctgaacag
     3301 gctgatggca tggttatcat caaaaccaag ctgatgggtg agatagagaa tgagtaaggc
     3361 acgcatgccg taaaaactga agtactccca gatttggatc gccacgatat agtatatcgc
     3421 gcgcggctgt gagggtgttt tcatgtgttc tccttatgag caaaaaaggg aagtggcaag
     3481 ccacttccct tgtacgagct aattattttt tgctttcttc tttcaatacc ttaacggtat
     3541 agcggccatc agcctgacgg tatgcaccgt gaatatcggt ttcaaagccc ggatagtgag
     3601 cgccgatttc acacagcatc tgcaggaact ccagaaccgg acggctttct tcggtgatca
     3661 tttcacccgg cattaccaga ggaactcccg gcggatatgg aaggatcata ttggcgttaa
     3721 tacgacctac catttcgtcg aggtaaactt cttcggtcat accgtgcagc tctttctgga
     3781 acgcagcata cggagtcatt accattgtcg gcagcacttc aaatgcgcga tacatcagat
     3841 ccggcagatt gtggtgaaca atcagtttgt ggatattctg agccagttcc tgaatacgca
     3901 tgttttcata gaattcagga tcttcacgat acagagacgg cagcatgttt ttcacacgca
     3961 ggttcaggtc gaacgcacgt ttgaagtcag tcagagcacg cagcaggctc agtgctttgg
     4021 tcttatcgat accgatgctg aacaggaaca gcaggttata cggaccggtt ttctcaacaa
     4081 cgatgccatg ttcgtcgagg tatttcgcca cgatgctggc cggaatacca aagtcgctca
     4141 tggtgccgtc tttttccatc cccggagtca gcagggtgac tttgatcggg tcaagataca
     4201 tgtgctcgtt atcgatgttt ttgaagccgt gccaggtgct gtcagaacgc agcggccagc
     4261 attcagtcgt atcgatatga tccggctgcc aaacatcaaa gaaccagcca tcagattccg
     4321 ttctcagacg tttgatctct ttacggaatt tgatcgcacg ttcaatggaa ccgttgatca
     4381 gacgcttacc agcattacct ttcatcatcg ccgcagcggt ttcagtggac gccacgatac
     4441 cgtagtgtgg agaagtggtg gtgtgcatca tgtaggcttc gttaaaggtt tcttcgttta
     4501 cgtcaccttt aacgtggatc atggaagcct gagagaacgc cgccagcagt ttgtgagtag
     4561 actgggtttc gtaaatcact ttcccttcta cacggccacc gctcataccg catttacctt
     4621 cgtaaatcgg tgagaagttg gtgtaaggca cccacgcgga gtcaaagtgg atggatttca
     4681 catccagtgt tttcttgatg aagtcggtgt tgtacagcag accatcatag gtagagttag
     4741 taattacagc atgtaccggc caggttgcgt ttggtgtttc cttcacgcgc ttagcaatgg
     4801 tagcgtgctg gaattcactc tgtgggatac caccaagaat accgtaagcg ttacgggtcg
     4861 ggcggaaata gattggcgta acatcgctca tcatcatcag gtgggtcagc gatttgtggc
     4921 agttacggtc aatcagaatg gtgctgcctg ctggagcaga gtacatacca acaattttgt
     4981 tcgcagtgga agtaccgttg gtcaccatgt agctgcgatc tgcgttaaag acgcgagcga
     5041 tatactgttc tgcttctttg tgtggaccac tgtgatccag cagagaaccc agttcagata
     5101 ctgaaatgga aatatcagat ttcatggtat tcggaccaaa gaaatcatag aacaggctac
     5161 ctaccgggct tttctggaat gcagtaccgc ccatgtgacc aggagtacag aaagtatatt
     5221 taccttcacg aacatattta aacagtgctt tagtcagcgg aggcagaata gtgttgatat
     5281 attcgtcagt ggtctgcttg atcttgttag caatatcttc agcagcacct agcgcatatt
     5341 caaagaagct aatctgtaaa cgcaggtcat tcaggcttac atcgagagtg gaatacgtat
     5401 tagcgaacgc gtacaacggc aggttctcgt tcattttgct aatttcttcg cacagctcga
     5461 gattatattt atcccagtca aaaataacgc cgcacagacg cgcattgttt tcgatcagtt
     5521 ttaataagtc gtcacggtcg ttcgggtaaa caatctggaa gttcagacgt tcaagcgcgc
     5581 gatgaagttc acggatgggt tcttctttaa aataaacccc catgtgattc aatattgcaa
     5641 taacgttcat agtcatatct ccaggtaaaa aaggcccctc ccaacacatg ggacaaaatg
     5701 aaaggaggag cctcggaaaa tacttttaat taatgtgcgt tagacgctgt ttggttatcc
     5761 attgagtggc tctggcgctc gtgcattttg cgagcgtaga acatcaggat aatcaggctg
     5821 acgatgaagg tacctgccag ctcgaaggag cttgcgccca tcagcgcgat gaagcagaac
     5881 acgcaaccca gtacagagca gatcaggctg acaaagttgc ggatgttaac gccttcaaaa
     5941 cgaatcaggt caacgcaaga gtagaagtac ggcagcatag tcagcagtac tgcgataccg
     6001 gtcagttcac cgaacaggtc agatgcttta ccaccggcag agttcatcag agtgatgagg
     6061 atcatcaggg cagtcatttt cactgcagcc agcagcagac cttttttcgg aataccgttg
     6121 ctgtcgactt caccataaac tttcgggaag ttaccgtcgt tagcggcacg tacacctgcc
     6181 tggcctacca acatcatcca ggagcccaga gaagtcaggc acgcaaaggc ggtgaatgca
     6241 gaaaccagcg gcgcagccca gttaccgagg atagttgaag cactgattgc aaacggagca
     6301 ccggaagccg ccattacaga agacggatac ataccggaaa gcacctgagt cgcagcgatg
     6361 taaacaatac ctgctaaacc agtacccagc atggttgcca gcggaacggt acgtttcggg
     6421 tttttaacca taccagtact tacagctgcg gattcaacac ccacgaaggc ccacaggcag
     6481 agcagaatac ttttaatgat cgcatgacca tcagtggtat ccgcagtatt ccagttagct
     6541 gcataagttg ccgcatcaaa ccaatgccag ccaacaatag cagtcatcac cacaggaata
     6601 agaaccagca ccagaccaat agtggttaaa cggcttaccc aggtaccgcc gagcatattt
     6661 acaaaggtaa atacccagac gatagcaata caggcgatac ccgccggaac aggatcattt
     6721 aatactggga agaaggtgga aagataagat acagcggtaa taccaatcgc caggttacca
     6781 atccagttag catggtaata aagaacacct gtctgaaaac caaatgcagg agaaatttct
     6841 ccggcataag caattgggcc accttgttgc gggttttttg ttgccagtcg ggcatataca
     6901 tacgccagcg acattgcacc aataatagag ataatccaac cccagatagc aataccaccg
     6961 atacttgcta ggttcgcagg taataatgca ataccgctcc ccatcatatt accggcaaca
     7021 acaccggtac aggcaaatag cccgatcttc ttggcagaac tcatgctctt ctcctaattt
     7081 catttttgaa tttggagtcc gggtcatgat gtataactat ttcctgacca gaccaaactg
     7141 gcgctaagat tactcacgaa aaaaggatta atcctaaaga ttaggtgaaa taacacaaaa
     7201 gtttctgtaa gtgagaactt gaggtttttt attaacacat caggatcgca agttgatatc
     7261 atgaaaagat aaacattcaa tttttacaat ggattgcgtg acattgtctg gttaaattta
     7321 tgtaataaaa attatgcggc aaataaattg ccgcaacata ttataccaac aggaacatac
     7381 aaaaactcaa caacaaatat tccctagcat gaatcaaccc gggagttact tattctgaag
     7441 cgagaaattt gtcgagataa ggtacaacat aaggaacaga agtctggaat ataccatttt
     7501 caatccagta aagggtgttt gcccctgggc gtaaattaaa ggcggtgaga tatgcatcag
     7561 ctgcttcccg attcatcccc ttcatttcat aaaccttgcc aagcaataca taatttagcc
     7621 aggacatttc aagatcaatg ccagtattta tcgcctggta agactcatct gttttacctt
     7681 ttaccagagc actgaccgct tttatttgat atataatgga caggttattc aattccggta
     7741 gtgtaacaat gttatctatt tctgtgttca gtgctgctaa ttgtttttca tctaaaggat
     7801 gttgagaatg gcgcacgata tcaactaatg ctttttctgc tctcgcgtag gtaaattctg
     7861 gagatgattg aacaatctca cctaataatt cactggcacg gttcaatgat ttatcatcgc
     7921 catgcagtaa ataatcatgt gcctgataaa aattagttaa taacgcacca cgatgaggca
     7981 aaatttgctg gagcgtctcc tgcattcgtt gtggccacgg ttggtttaac gcttttgata
     8041 aactctccag taaatcattt tgaatcgcca gctgattacc gttagtgatg acataacgtt
     8101 tatccagcat ggttgaacca tctgcattgt ctaccaattt tatcgacata aagcattgtt
     8161 gagcacggta ttggcgctga ttaacaaacg caatagataa tgttttaccg gaactgctcg
     8221 gttcatcaat gttgtagttg attttgtcat gcaccataaa ggtggagaag gtgttaagtg
     8281 atgtcgccac caaatcaccc acgcctatcg cgtaagagag ctgatacggg gaactccagc
     8341 tgttacaact tttatttacc atattaatgt caatatcgcg tggattgagc aaaatacgcg
     8401 atttgctcat cggaagacgt gtatcaagac ttgaaaacgc taccagtgct acacagatac
     8461 ctaaggacaa caggaaaaaa aaccataccc aaaaggtagt gaatcgtttg cttttaactg
     8521 gggattgttc aggtggcgtt gcggtgtttt gaatgttaag actgtgggag ggagaatctg
     8581 tggcaggaac cgcctctggt atagggggag gcgaagatag cattatttcc tctccctctt
     8641 cttcgctgta ccagataacc ggcaccatta atttatagcc gcgctttggt acagtagcga
     8701 tatagacagg actatcttca tcattatctt ttaatgactt acgtagttct gagatactct
     8761 gcgtcacaac atgattggtg acaatacttc tcttccagac attatcgata agttcatccc
     8821 tgctaagtac ttcgccactg tgttgagcaa agaaaaccag aagatcgatt aatctcggct
     8881 caagggtaag ttgacgccca ttgcggctaa tttggtttat ggacggagta acaagccatt
     8941 cgccaacgcg aactacaggt tgttgcataa tagaaactca ttcgaaaagg gaatgatgca
     9001 atgataattg ccacaaccta tttttaccat ctatagatgg gtttatttac atattattgg
     9061 tgaatgcaag acgttatttt taccagccat aaacttcttg attacatggt attaagaaag
     9121 gattttactg agaaccagaa gtaatattcc ttaccatcaa aattcatcat ctttgccaaa
     9181 gaaaaatgtt cagaaaataa tccatggaaa attgtccgga gcgcttacta ttttaatgga
     9241 ttgttagtct ttgcatgagc aagcggactg atacatttct ctttgttctc attcagaaaa
     9301 tctcattagt cgccgttcag accggtgttg tgctttatgg atgcgttacg ctcctgatga
     9361 cgtcatttga cgttcaacag catcacgggg ccgcacgaca tttcacgtca gttagtgcta
     9421 tagctaagga gcaaattttc ccgaattggg atatgcccgc aaattgctgg tgatgtgggg
     9481 agaatctggt tgagttcggg agaattgatt tggaggcaga acgcttaaat cgtggtgtcc
     9541 tgaaacgaaa aacggacctc cgtggaggtc cgtttatatg aatttggtgc ccggactcgg
     9601 aatcgaacca aggacacggg gattttcaat cccctgctct accgactgag ctatccgggc
     9661 aacggggcgc attaaaccgt aatctgcaca tctcgtcaac ctaatttcag taaaagcgat
     9721 tcaactgctt aagattgcgg caaatcgctt cttttctgtg tttctgtcag gtcaatgcgc
     9781 caccctggcg gcagcgggcg aagcgcagga tatcttccgc cagccgatgt gcagtgtcga
     9841 catccgcctg gctacgattc accagcatcc ggctcaggca tccttccagc accagttcca
     9901 tctgctttgc taccatcgcc ggatcgtcaa cttccagcgt ggttaacagt tcgtgggtga
     9961 aatcgtaggc cgcgcttttt tgctgatcgg ccagttgatg aatagggtgg ccaggatcgg
    10021 gataaaacgt acaggcagcg ataaacagac agcccggata gcggttgttt ttaacgcact
    10081 ccgataacgc ctgataacgt gccagcagct tttgttcggc ggtttgcgtt tcgtccagca
    10141 tcagctgacg acgccagaca tctatctgtt ggctaagata acgcagcgca tcgtagagga
    10201 ttgcctcttt gtctggccag aagcggcgca gctcgtccag tggataatcc acacgttcag
    10261 caaccatctc cagcgtggtg ttggcaatcc cttgtaattc taataatttc agggcttctc
    10321 ccagtacatc ttcacgttgc acgctatttt cctccgtctt tcccactgca agtgtcgttc
    10381 acggttggcg atcgcgcaaa tgtgcgctga aggtttcagc atccataaag cccgtgacgc
    10441 gtgcttgtgg atgctcctgg ccttgtccgt caaaaaagag aattgtcggt aggccaagga
    10501 cattaagatg ctttaacagc gccacatctt gtgcgtcgtt ggccgtgacg ttggcctgaa
    10561 gtaagaccgt gtctgctaac gctttttgca cctgcgggtc gctgaaggtg tatttctcaa
    10621 actctttaca ggcgacgcac cagtcggcat aaagatctaa catcaccggt ttgcctttgg
    10681 cttcaacgag cgcctgattt aactcatcta ccgttttgat ttgtgtaaag ttgagatgcg
    10741 tctgagtttg cgcggtatgc gtcgcaccaa atgcccaatc ctgaagtggg cgcacgctaa
    10801 ccaatgccgc tgccagcagg attatttgca ccacgcgcat ccagccgcgt ttggcctgta
    10861 ggctggtgat aaaggcccag ccaaagaatg cgacaccaag cgccgaccac aagcgtaatc
    10921 cccatatatc accaatcact cgctccagca ggaagaccgg cagtgcgagg atcacaaaac
    10981 caaacgcggt tttgacttgt tccatccacg ggccgctttt cggcagcaag cggttaccaa
    11041 agacggtaat tagcatcagc ggcaggccca tgcccaacgc atagagataa agcgtgccgc
    11101 cgcccagcca catgttcccg ctttgggcga tatacagcag aatcgcgcta agcggtgcgg
    11161 tggtgcatgg tgaacagatc agtccggcaa tcgcccccat aacaaacaca ccgccagatg
    11221 agccgccctg ttggcgattg ctcatcagcg tgagacgtgt ttgcagcgaa gaggggagtt
    11281 gcagggtaaa caagccaaac attgacatcg ccagcaaggt aaagacgata gcgaggccaa
    11341 tgagcacgta tgggtgctgt agcgccgcct ggaactgtaa ccctgcggcg gcaactacca
    11401 gacccagcgc cgtgtaagtc agtgccatcc cctgcacata aataaaggtc agcaacaatg
    11461 ctctggcagt ggaaagccgc tgtttaccgc ccagcacgat gccagaaatc agtgggtaca
    11521 ttggcagcac gcatggcgta aaggcgatac caataccgat caacaacgcc cagagcgcgg
    11581 aaaagggcaa ttgcgctgtg ggctgctctt gctgcggaac agacacaggc tgtggcgctg
    11641 cgttgttggc gaccacttcg cttaacggaa cggttttggt ttctggcgga taacagaaac
    11701 cggcatcagc acagccctgg taggtgacag ttaacatcgc tcccgcactt gcctggttga
    11761 tggttacggg aagcgtcagc cgatcgcggt aaatctcgct tttgccgtaa aactcatctt
    11821 catgccagac gccttgcggc agctgcacgt cggcaatttt cgcgtgttcc ggcgtaatgc
    11881 ggatctgttt acggtagagg tagtaaccgt ctttgatctg ccaggtcaga ttaaggtcat
    11941 gttggttttg ctgaaaatca aaagcaaagg cttgatccgc ggggacaaat tgtgaacgtc
    12001 ccggcgcgtc gaataatccg gcaaaaacgg aagtgctgca aagtagcagg atcagcgtaa
    12061 agatgcgttg agccatgaga ggtaatctgt gtctccgtgt gtaacaggta aaaccagaag
    12121 ttccggggtt tgatatggat gatgagactt caggcattcc agcagtgcct gctggtgaga
    12181 tacggtagtt tttaaaatca tctgcacttc gtattcttgc tccagcttac cttcccagta
    12241 atagagagag gtagcaccgg ggatcaaggt cgcgcaggcc gccagttttt ccgccagcac
    12301 tttggcagct aaatcctggg ctgtcgcttc atctggtgcc gtacatagca ccacgacaga
    12361 cgcggtattc gaactttttt catcaagcat aaacacctcg caagaacaga tggaaccgca
    12421 agagagaggt cactatacaa cgtacggggg aaggatgtta gtcggcggga aagaaagcgg
    12481 ggcgtgaacg ccccgcgatt ggtcattaca gcatgaagct acccagcacg aagccgaagc
    12541 aaacggccag ggcaacaccc agagtacccg ggatgaagaa cggatggttg aagacgaatt
    12601 taccgatacg ggtagtaccc gtgtcatcca tctgtaccgc agcaaccagc gtcgggtagg
    12661 tcggcagaat gaacagacca gacaccgcag cgaaagaagc aacagcggtc agcggtgaaa
    12721 cgttcagtgc cagagccatc ggcatcagtg cttttgcggt tgcagcctga gagtacagca
    12781 gagcagaagc aaagaagaag atgacggcca gcagccacgg atgaccctga atcacttcac
    12841 cagcggtatc tttgatccag tcgatgttgt tggaaacgaa agtatcgccc agccacgcaa
    12901 cacccaggat acaaatacag gcgctcatac ctgctttgaa ggtgctggag ttgaggatgt
    12961 tgtcggtatc gactttacag ataacggtgg tcagagttgc aacgctgagc atgatgatca
    13021 ggattgcgtt ggtggtgttc atcagcggtt tttcaaccag acccatgctt gggctgttga
    13081 tgattgcata gataaccacg ccaactacac ccagcaggaa cagccagacg gacgtttttg
    13141 caccggattt gatttcaatc tgcttttcac cgcgcagttc aaccaggccc tcttccagac
    13201 gcttgcgata aatcggatcg tcagagagtt tggagttgaa cagcatagtg accaggaagg
    13261 acatcaccag aaccgccagc agggtggacg ggatgaccac ggagagcaga tggaggtagc
    13321 tgatgccatg accttccatc acggaagaca tgtaaaccac tgccgctgag attggcgatg
    13381 cggtgatcgc aatctgcgcg gataccactg cagtagacag cggacggcaa ggtttaacgc
    13441 cttgttcctt cgcaacttca gcgataactg gcagtgtcgc cagagagatg ttgccagtac
    13501 cagcaaagat agtcaggaaa taggtcacga tcggtgcgag gatcgtgatg tatttcgggt
    13561 tacggcgcag cagcttttct gtctgatgaa ccagatagtc cagaccgcca gcaacctgca
    13621 tggcagaaat agcggcgata accgccatga taatggaaat gacatcgaac gggatgttac
    13681 cgggtttaac gccaatagcg gcaagaacca gcacccccaa tccgcctgca aaaccaatac
    13741 ctattccccc caatctggcg cccaagaaga tcgccagcaa aactatgatg agttctacaa
    13801 ctagcatatt agccttcctt gttttttaac aagttgatat tagattgtta tttttaagtt
    13861 actgctcaca agaaaaaagg cacgtcatct gacgtgcctt ttttatttgt actaccctgt
    13921 acgattactg ttcgctttca tcagtatagc gttttgcttt gtaagccggg tgcatcagat
    13981 tctgtacgga gaaaatatcg tcaagttccg cttcagtcaa cagaccgcgt tccagaacga
    14041 cttcacgtac actcttaccg gtttcggcac agattttacc cacgatgtca ccgttgtggt
    14101 gaccgatgaa cgggttcagg taagtaacga taccgataga gttgtaaacg taaccttcgc
    14161 acacttcttt gttagcagtg atgccgttaa tgcatttttc cagcaggttg tagcaagcgt
    14221 tggtcagaat gtgaacggat tcgaacatag cctggccaat gaccggctcc ataacgttca
    14281 actgcagctg acctgcttct gctgccatgg taacagtggt gtcgttaccg atgactttga
    14341 agcatacctg gttaaccact tccggaacaa ccgggtttac tttagctggc atgatggaag
    14401 agcccgcctg cagttccggc aggttgatct cgttcaggcc ggcacgtgga ccagaagaga
    14461 gcaagcgcag gtcgttacag attttggaca tcttcacagc caggcgtttc agcgcgccgt
    14521 gaaccataac ataagcgccg cagtcagagg tcgcttcgat caggtcttca gccggtacgc
    14581 atgggaagcc agtaacttca gccagttttt tcactgccag cggagagtac tctttcggcg
    14641 tgttcagacc agtaccgatt gctgttgcgc caaggttaac ttccagcagc agttcagcgg
    14701 tacgttggat gtttttcact tcttctttca gcaggatgct gaaagcgcgg aattcctgac
    14761 cgagggtcat cggtactgcg tcctgcagct gggtacgccc cattttcagg atgtcctgga
    14821 attcgacagc tttacgttca aagccttcac gcagttggtt aatcgcatct accagcttaa
    14881 tcagggaaga gtaaactgcg atacggaaac cggtcgggta ggcgtcgtta gtggactgac
    14941 atttgttaac atggtcgttc gggttcaggt actgatattc acctttctgg tgacccatca
    15001 gttccagacc gatattggcc agcacttcgt tggtgttcat gtttacggaa gtacctgcgc
    15061 cgccctggta gacgtctacc gggaactgat ccatgcattt tccgttgttc aggacttcat
    15121 cacatgcggc aatgatggca ttcgctacac ttttaggaat ggtttgcagc tctttgtttg
    15181 ccatagctgc ggctttttta accattacca taccgcgaac aaattcagga atatcactga
    15241 ttttgttgtt gctgatatag aagttttcaa tcgctctcag agtgtgaaca ccatagtagg
    15301 catcagctgg aacttccctg gtacccaaca gatcttcttc gatacgaatg ttgtttgaca
    15361 tgtgaacctt ctttttcaag ctgccaatga tttgctttaa acacacagaa tatatgtggt
    15421 ttcgaatgtt tttcgaccga cgattatccc ctgcatcgac cgaatacccg agatcatatg
    15481 ctgcttgagg atttctaccg taatctggat cactttaagt gtcggttttt accccttaat
    15541 tattaatttg tgaaatagat caccgctttg ggattactac caaaaatagt tgcgcaaaca
    15601 tcttgaaatt ttgctaatga ccacaatata agctgaacgc gattcgcaac ccattcaggt
    15661 agccgggttt taccggctgc tattacagga gaaacctttg cgctggttac cttttattgc
    15721 cattttcctt tatgtctata ttgaaatttc aatctttatt caggttgccc atgtattggg
    15781 ggtattgctg accctcgtgc tggttatatt cacgtcagtt atcggtatgt cactggtacg
    15841 taaccagggc tttaagaatt tcgtgctgat gcagcaaaaa atggcggcgg gtgaaaaccc
    15901 tgcggcggag atgattaaaa gtgtttcgct gatcattgct ggtttgctgc ttttattacc
    15961 gggctttttc accgacttcc tcggtcttct gcttttattg ccgccggtgc aaaagcatct
    16021 gacagtgaag ttgatgccac atttgcgctt ttctcgcatg ccaggcggcg gttttagcgc
    16081 cgggaccggt ggcggtaata cttttgatgg tgagtaccag cgaaaggatg atgaccgcga
    16141 ccgccttgat cataaagacg atcgccagga ttaatgtcga aacgccggat tatgtggtta
    16201 tgccattttc cggcgttttt cgttttggca gaaacagcca taaccccgcc agcataatca
    16261 gcgcatagag acttttccag ccgaccattg tcagtaacag aacgcataac agcccgccaa
    16321 ccaccgccag tagtcgataa cgtccttgca ataatttaca gcctgccagc atgcataaca
    16381 gataaatcat aataaagatg ccattggcat aaataataag agcgtccaga ttgatctcta
    16441 aagcatgaat caccaacgtg ctcaccacgc agcagccgag caccgcattg agggcattat
    16501 tcgggatatg gcgagaagag aggcgtgcca ggtagtggtc aggattatgt tgcgcctgcg
    16561 accagaccag gcgggcgaag ctctgtatat aaatgttgag actggcaaag caggccagat
    16621 agccaattac gcaggcaatc cataacgctc ctacaccgaa cagttgcact acaatttttg
    16681 gaagcgatgc tgccgcggcc atttgttcac cataggcgtc gaagtgtaag acgactaccg
    16741 tacagcccca gtagactaat cctgccagca gcagaccaat catcaaagca cgaggaaaat
    16801 cacgctctgg atttttaaat tccgaggcaa gatgggcaaa tgcttcaaga ccgacaaaac
    16861 accagaacat cactgataac gcagcaaata acccggtaag ttcgatattt cctggcgcag
    16921 ggaaggggat attcgcaggt ttgatatcgc ccgcccacca gatagcgaca atcagcgcga
    16981 cgataagtcc ggcaataacg gtttgtagat ttgcactgga actggcaccg cgagtaccga
    17041 tataccacac caacgccagc gtaccgagtt ctgccaacaa cagttgccag ctatgccagt
    17101 caaacatcgc ctggccgaac ccggcagcaa tttgtagtgc ggcgggtaaa cccacaggaa
    17161 tgactgataa aaacagccag ccggtgactc gctcaagccg cgaaccaaac gccataccta
    17221 cgaagtgcgc gacgccgcct gcgctgggat agtggcgacc cagaatcgca aacacaatcg
    17281 caatcgggaa cactaagata atcaaaacgg gccacgccca caggctgtta ttgcccgcta
    17341 ccagtgcagc taacgcagga acggcaaaca cgccagtgcc taataatgac gtcgatagca
    17401 ggccaacgcc ctgggccagc cccagttctt gtttgagtcc actcatgggt tgatgtccga
    17461 ttgcgcccaa attttgggca actgcgtaga ttttcgatgg tagcacaatc agattcgctt
    17521 atgacggtga tgaagaaatt gcgatgaaat gtgaggtgaa tcagggtttt cacccgattt
    17581 tgtgctgatc agaatttttt ttctttttcc cccttgaagg ggcgaagcct catccccatt
    17641 tctctggtca ccagccggga aaccacgtaa gctccggcgt cacccataac agatacggac
    17701 tttctcaaag gagagttatc aatgaatatt cgtccattgc atgatcgcgt gatcgtcaag
    17761 cgtaaagaag ttgaaactaa atctgctggc ggcatcgttc tgaccggctc tgcagcggct
    17821 aaatccaccc gcggcgaagt gctggctgtc ggcaatggcc gtatccttga aaatggcgaa
    17881 gtgaagccgc tggatgtgaa agttggcgac atcgttattt tcaacgatgg ctacggtgtg
    17941 aaatctgaga agatcgacaa tgaagaagtg ttgatcatgt ccgaaagcga cattctggca
    18001 attgttgaag cgtaatccgc gcacgacact gaacatacga atttaaggaa taaagataat
    18061 ggcagctaaa gacgtaaaat tcggtaacga cgctcgtgtg aaaatgctgc gcggcgtaaa
    18121 cgtactggca gatgcagtga aagttaccct cggtccgaaa ggccgtaacg tagttctgga
    18181 taaatctttc ggtgcaccga ccatcaccaa agatggtgtt tccgttgctc gtgaaatcga
    18241 actggaagac aagttcgaaa acatgggtgc gcagatggtg aaagaagttg cctccaaagc
    18301 gaacgacgct gcaggcgacg gtaccaccac tgcaaccgta ctggctcagg ctatcatcac
    18361 tgagggtctg aaagctgttg ctgcgggcat gaacccgatg gacctgaaac gtggtatcga
    18421 caaagctgtt accgctgcag ttgaagaact gaaagcgctg tccgtaccgt gctctgactc
    18481 taaagcgatt gctcaggttg gtaccatctc cgctaactcc gacgaaaccg taggtaaact
    18541 gatcgctgaa gcgatggaca aagtcggtaa agaaggcgtt atcaccgttg aagacggtac
    18601 cggtctgcag gacgaactgg acgtggttga aggtatgcag ttcgaccgtg gctacctgtc
    18661 tccttacttc atcaacaagc cggaaactgg cgcagtagaa ctggaaagcc cgttcatcct
    18721 gctggctgac aagaaaatct ccaacatccg cgaaatgctg ccggttctgg aagccgttgc
    18781 taaagcaggt aaaccgctgc tgatcatcgc tgaagatgtt gaaggcgaag cgctggcaac
    18841 tctggttgtt aacaccatgc gtggcatcgt gaaagtcgct gcggttaaag caccgggctt
    18901 cggcgatcgt cgtaaagcta tgctgcagga tatcgcaacc ctgactggcg gtaccgtgat
    18961 ctctgaagag atcggtatgg agctggaaaa agcaaccctg gaagacctgg gtcaggctaa
    19021 acgtgttgtg atcaacaaag acaccaccac catcatcgat ggcgtgggcg aagaagctgc
    19081 aatccagggc cgtgttgctc agatccgtca gcagattgaa gaagcaactt ctgactacga
    19141 ccgtgaaaaa ctgcaggagc gcgtagcgaa actggcaggc ggcgttgcag ttatcaaagt
    19201 aggtgctgct accgaagttg aaatgaaaga gaaaaaagca cgcgttgaag acgccctgca
    19261 cgcgacccgt gctgcggtag aagaaggcgt ggttgctggt ggtggtgttg cgctgatccg
    19321 cgtagcgtct aaactggctg acctgcgtgg tcagaacgaa gaccagaacg tgggtatcaa
    19381 agttgcactg cgtgcaatgg aagctccgct gcgtcagatc gtcctgaact gcggcgaaga
    19441 accgtctgtt gttgctaaca ccgttaaagg cggcgacggc aactacggtt acaacgcagc
    19501 aaccgaagaa tacggcaaca tgatcgacat gggtatcctg gatccaacca aagtaacccg
    19561 ttctgctctg cagtacgcgg cttctgtggc tggcctgatg atcaccaccg agtgcatggt
    19621 taccgacctg ccgaaaaacg atgcagctga cttaggcgct gctggcggta tgggcggcat
    19681 gggtggcatg ggcggcatga tgtaattgcc ctgcacctcg cagaaataaa caaacccccg
    19741 ggcagaaatg tctgggggtt tttcttttgg tcatctttct agtataagat tcagacacgg
    19801 acgacgcgag tggcgtccag ctcattgatt atggggaata acatgcacgt aaaatactta
    19861 gcagggattg tcggtgccgc gctactgatg gcgggttgta gctccagcaa cgaattgagt
    19921 gctgccggtc agagtgtacg cattgtggac gagcagccag gcgcagagtg ccagctgatt
    19981 ggtactgcga caggtaagca aagcaactgg ctttccgggc aacacggaga agaaggcggt
    20041 tctatgcgcg gcgcagcaaa cgatctgcgc aaccaggcgg ctgcaatggg cggtaacgtg
    20101 atttatggca tcagtagccc gtcgcaggga atgttgtcca gttttgtccc gacggatagc
    20161 cagattatcg gccaggtata taagtgcccg aactgatgtg atggtgacaa acgtataatt
    20221 gccagatgcg cttcgcttat caggcctaca taaggcattg caactgattg aatgcttgag
    20281 aatttgtagg ccggataagg cgtttacgcc gcatccggca tgaccaacga tcacgttatc
    20341 agtcatttca ggctggatgg ctccagcctc gcattagcaa agatgagatt atttcgcctg
    20401 tggtgcagtt ttgctggtgg attgaacacg taagtgatgc agcgcgcgta agcattgatc
    20461 ctgggttagc ggttgggcgt tattcgccgc tacggtgcga acatagacct gacatggcac
    20521 acccgcgagt tttttcagtc gtgggctgaa atcaagcatt cggcgagtga tcgcctctat
    20581 ttctttaccc ggcgtttctt cacgcgtttt caccactgcg ccgctaaatt gttctttgcc
    20641 actttcatct ttaaagcggt aaagcaccgc cagataatgg tcgaacaagt tatgtttcca
    20701 ttccggttgg gaataggtgt catattccag attgccattt tcctggcagt caacgtcata
    20761 taacggcagg aaatctaaca gtgaggtaat acgtaatgcc agttcacgca tttccgcatt
    20821 attaattgca tgaatgattg ctcgcgctag catttcaatc tgcaactcat tgacgactaa
    20881 ctcacatttg ctaccgtcat gtagcgttaa tacgaaggta agattttcgc cttgattatc
    20941 ggtaagttcc agcgtgttaa cccgacgatt aatatcggca tttttaagct catcaaccag
    21001 aatttctggg atgttttctg ccattttctt aatgacttta tcgcgagctt gctcatactg
    21061 aaggcgtgca gcagcatcca gtttatgctt ttgatgcaga cgactttcca gcgcaatcag
    21121 caggtcgcgc agttccatga cagacataaa gaacaacgat tctttattgc gaggttcctt
    21181 aattttcagc gccagtgcaa tgaaattatt actcttgcga ataacaccgg tattaacacc
    21241 cttgatgctt atggc
//
LOCUS       DAPGOF010000082        20842 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.082, whole genome shotgun sequence.
ACCESSION   DAPGOF010000082 DAPGOF010000000
VERSION     DAPGOF010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.082"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            157..606
                     /gene="alaE"
                     /locus_tag="RHV13_002620"
     CDS             157..606
                     /gene="alaE"
                     /locus_tag="RHV13_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HDV1242143.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(643..987)
                     /locus_tag="RHV13_002621"
     CDS             complement(643..987)
                     /locus_tag="RHV13_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HDV1242144.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKTYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1139..1468
                     /locus_tag="RHV13_002622"
     CDS             1139..1468
                     /locus_tag="RHV13_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HDV1242145.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            1716..1961
                     /gene="nrdH"
                     /locus_tag="RHV13_002623"
     CDS             1716..1961
                     /gene="nrdH"
                     /locus_tag="RHV13_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HDV1242146.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            1958..2368
                     /gene="nrdI"
                     /locus_tag="RHV13_002624"
     CDS             1958..2368
                     /gene="nrdI"
                     /locus_tag="RHV13_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDV1242147.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIAQKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            2341..4485
                     /gene="nrdE"
                     /locus_tag="RHV13_002625"
     CDS             2341..4485
                     /gene="nrdE"
                     /locus_tag="RHV13_002625"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDV1242148.1"
                     /translation="MATTTAERLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESALNRYSRDFVITLFAHAHASGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLATQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGVVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADVPISQHYDEL
                     VADERIRKKYINARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYGENLDYARTGHDISCNLGSLNIAHTVDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGQTFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            4495..5454
                     /gene="nrdF"
                     /locus_tag="RHV13_002626"
     CDS             4495..5454
                     /gene="nrdF"
                     /locus_tag="RHV13_002626"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDV1242149.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETLWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            5809..7011
                     /gene="proV"
                     /locus_tag="RHV13_002627"
     CDS             5809..7011
                     /gene="proV"
                     /locus_tag="RHV13_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HDV1242150.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            7004..8068
                     /gene="proW"
                     /locus_tag="RHV13_002628"
     CDS             7004..8068
                     /gene="proW"
                     /locus_tag="RHV13_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HDV1242151.1"
                     /translation="MADQNNPWDTTPAADSAVQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            8125..9117
                     /gene="proX"
                     /locus_tag="RHV13_002629"
     CDS             8125..9117
                     /gene="proX"
                     /locus_tag="RHV13_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HDV1242152.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            9309..10493
                     /locus_tag="RHV13_002630"
     CDS             9309..10493
                     /locus_tag="RHV13_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1242153.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            10617..11354
                     /gene="ygaZ"
                     /locus_tag="RHV13_002631"
     CDS             10617..11354
                     /gene="ygaZ"
                     /locus_tag="RHV13_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HDV1242154.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            11344..11679
                     /gene="ygaH"
                     /locus_tag="RHV13_002632"
     CDS             11344..11679
                     /gene="ygaH"
                     /locus_tag="RHV13_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HDV1242155.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            11770..12300
                     /gene="emrR"
                     /locus_tag="RHV13_002633"
     CDS             11770..12300
                     /gene="emrR"
                     /locus_tag="RHV13_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HDV1242156.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            12427..13599
                     /gene="emrA"
                     /locus_tag="RHV13_002634"
     CDS             12427..13599
                     /gene="emrA"
                     /locus_tag="RHV13_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HDV1242157.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAQTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            13616..15154
                     /gene="emrB"
                     /locus_tag="RHV13_002635"
     CDS             13616..15154
                     /gene="emrB"
                     /locus_tag="RHV13_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HDV1242158.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            15412..16119
                     /locus_tag="RHV13_002636"
     CDS             15412..16119
                     /locus_tag="RHV13_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase family protein"
                     /protein_id="HDV1242159.1"
                     /translation="MNTQLKYGRTWHYPFSPGTTSDDRINADYWQDLQAITQLVHTEK
                     LDGENNCLNRYGVFARSHAAPTQSAWTYKIRQRWQLLKNDLGDLELFGENLYAVHSIE
                     YRALEQDFYLFAVRCQDMWLSWEEVQFYAALFDFPCVPEISGPQPGNDEKSWQRDFLA
                     LTNARGAFDPWDTQTGQPCTLEGIVSRNRDAFSVADFSHNVFKYVRKNHVKTTVHWKR
                     HWQRARMAHEFAYGEQS"
     gene            16116..17228
                     /locus_tag="RHV13_002637"
                     /pseudo
     CDS             16116..17228
                     /locus_tag="RHV13_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625743.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(17277..17792)
                     /gene="luxS"
                     /locus_tag="RHV13_002638"
     CDS             complement(17277..17792)
                     /gene="luxS"
                     /locus_tag="RHV13_002638"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDV1242160.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(17942..19498)
                     /gene="gshA"
                     /locus_tag="RHV13_002639"
     CDS             complement(17942..19498)
                     /gene="gshA"
                     /locus_tag="RHV13_002639"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HDV1242161.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADGKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(19571..19999)
                     /locus_tag="RHV13_002640"
     CDS             complement(19571..19999)
                     /locus_tag="RHV13_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDV1242162.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(19996..20562)
                     /gene="yqaB"
                     /locus_tag="RHV13_002641"
     CDS             complement(19996..20562)
                     /gene="yqaB"
                     /locus_tag="RHV13_002641"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HDV1242163.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         4903 a         5404 c         5310 g         5225 t
ORIGIN      
        1 aatattttat ctacaaaaac tgactaaata aaaatttttc actaattgat tagtcatagc
       61 cagcgatata cgctatgcga aaatgcagat ggcaatgaga tccactgttt tcatctccat
      121 taacatccca ttacgctttt attaaggagc attagcatgt tctcaccgca gtcacgcttg
      181 cgtcatgcag ttgcagatac gttcgcgatg gttgtttact gttctgtcgt gaacatgtgt
      241 attgaagttt tcctctccgg aatgagcttc gaacagtctt tttattccag attggtagcg
      301 attccggtga acatcttaat tgcatggcca tacggtatgt accgcgatct gtttatgcgc
      361 gcggcacgca aagttagccc gtcgggctgg ataaaaaatc tggcggatat cctggcttat
      421 gtgacgttcc agtcaccggt gtatgtggcg atcttgttag tggtgggcgc agactggcat
      481 cagattatgg cggcggtcag ttcaaacatc gttgtttcga tgttgatggg ggcggtttat
      541 ggctacttcc tcgattattg ccgccgactg tttaaagtca gccgttacca gcaggtaaaa
      601 gcctgactct tcttttcgcg actggcatcg ccagtcgcgt ctttaactgg cttcgccaaa
      661 taaaccattc aaataacgtt caagcgcaac acgcgaacta aagccatgcg ggataccgta
      721 atgctcgtgt cgctcgcctg ctaaatagag cggaaattgt tgataatgat cgcaagtttt
      781 aatatcggaa gcgggttcag ccagtgtcga actacgttct tttaatgttg gatgaattac
      841 cagtgcggta cgtcccattc gcgcttcgcg attaacatag acataatttt ccccacggcg
      901 gtaaccatac gttttctggg ttaccacatc gacagtaaaa ccgacttttt caagtacgcg
      961 cgccacctcg tctggtcgta aatacatatt tgatcctcgt tattatcaac cgcgggccta
     1021 ccttacctga ttgcgcattc acaacgcttt cagaaaagtc cataaagccg cgatgaacct
     1081 cagttaagaa aattatggtc tacactgaaa attacatcga attctaatgg gggatcatat
     1141 gtttaaccga ccgaaccgca acgacgttga tgatggcgtg caggatatcc agaatgatgt
     1201 caatcaatta gctgacagcc tcgaatctgt attgaagtcc tggggcagcg acgccaaagg
     1261 ggaagctgaa gccgcacgca gtaaagctca ggcattgctg aaagaaacca gggcgcgaat
     1321 gcatggtcgt actcgcgtcc agcaagccgc acgcgatgcc gttggctgcg cggattcttt
     1381 tgttcgtgaa agaccctggt gtagcgtggg tacagcagct gcggtaggta tttttatcgg
     1441 cgcactgtta agtatgcgca aatcgtagtg caacaatgat aataaatacg cgtctttgac
     1501 cccgaagcct gtcttcgggg tttctttttg cctggtgaat cacaaaaatc cccctacccc
     1561 gtcacgctca tatccagggt aatttcgacc actatttgct atatattgtg tggttgaatc
     1621 ttttttcaac tacatctagt atctctgtat caacagagag acaacccgac gcgtatcatc
     1681 gcgccgtatc ttcattttaa acggaaatac gaatcatgcg cattactatt tacactcgta
     1741 acgattgcgt tcagtgccac gccaccaaac gggcgatgga aaaccggggc tttgattttg
     1801 aaatgattaa tgtcgatcgc gttcctgaag cggcggaagc gttgcgtgct cagggctttc
     1861 gtcagttgcc ggtagtgatt gcaggcgatc ttagctggtc tggtttccgc ccggacatga
     1921 tcaatcgtct gcatccagcg ccacacgcgg ccagtgcatg agccagctcg tctacttttc
     1981 cagcagctcc gaaaatacgc agcgttttat cgaacgttta gggctgcccg cggtgcgcat
     2041 cccgctcaat gagcgggaac ggattcaggt agacgagcct tacatcctga tcgtgccctc
     2101 ttacggcggc ggcggtacgg ctggcgcggt gccacgacag gtaattcgct ttttaaacga
     2161 cgagcacaac cgggcgttgc ttcgcggcgt tattgcttcg ggtaatcgca actttggtga
     2221 ggcgtatggc cgtgccggag atgtgattgc tcagaaatgc ggcgtgccgt ggctgtaccg
     2281 ttttgaactc atgggtacac aaagcgatat cgaaaacgtt cgtaaaggag taaccgaatt
     2341 ttggcaacga caaccgcaga acgcctgacg caggaaacga tggattatca cgcgctgaat
     2401 gcgatgctta acctctacga tagcgcaggt cgcattcagt tcgataaaga ccgccaggcc
     2461 gttgacgcct ttattgcgac gcatgtgcgt ccgaacagtg tgaccttcag tagccagcag
     2521 cagcgcctga actggctggt taacgaaggt tactatgatg aaagtgccct taatcgctac
     2581 tctcgcgatt ttgtcattac gctgtttgcc cacgcgcacg ccagcggttt tcgtttccag
     2641 acattccttg gggcatggaa gttttacacc agctatacgt tgaagacatt cgacggtaag
     2701 cgttatctgg aagattttgc cgatcgagtc acgatggtgg cgctgacgct ggcacaaggc
     2761 gatgagacgc tggcgacgca actgactgat gagatgctgt ctggacgctt tcagccagcc
     2821 acgccaacat tcctcaactg cggtaagcag cagcgcggcg aactggtttc ctgtttttta
     2881 ctgcgtattg aagacaatat ggagtcgatt ggtcgggcgg taaattccgc actgcaactg
     2941 tcgaaacgcg gcggcggcgt ggcatttttg ctgtcgaatc tgcgggaagc gggcgcgcca
     3001 attaaacgta ttgaaaatca atcttctggc gtgatcccgg tgatgaaaat gctggaagac
     3061 gcattttcct atgccaacca gctcggcgcg cgtcaggggg ctggggtagt ctatttacat
     3121 gctcatcatc ccgatatcct gcgttttctc gatacaaaac gggaaaatgc cgacgaaaaa
     3181 atccgcatta aaacactgtc gctgggcgtg gtgatcccgg atatcacttt ccatctggca
     3241 aaagagaatg cgcagatggc gctgttttcg ccttatgacg ttgagcgggt ttatggcaag
     3301 ccgtttgccg atgtgcccat cagccaacac tatgacgaac tggtcgccga tgaacgcatc
     3361 cgcaaaaaat acatcaacgc ccgtgatttc ttccagcgac tggcagaaat ccagtttgag
     3421 tccggctatc cctacatcat gtatgaagac acggttaacc gtgctaaccc tatcgccggg
     3481 cgcataaata tgagcaatct ctgctcagaa attttgcagg ttaacagcgc atcagagtat
     3541 ggcgagaatc tcgactatgc ccgtacaggc catgatattt cctgcaattt aggttcgttg
     3601 aatattgccc acaccgtgga ttctcccgac tttgcccgca cggtagagac tgccgtgcgc
     3661 ggtttaacgg cagtatcaga tatgagtcat atccgcagcg tgccgtccat cgaagccgga
     3721 aatgccgcct cgcacgccat cggactgggg cagatgaatt tacacggtta tctggcgcga
     3781 gaaggcatcg cttatggttc gccggaagca ctggatttca ccaatctcta tttctatacc
     3841 atcacctggc acgccctgcg tacctcgatg ttgctggcgc gcgaacgcgg tcaaaccttc
     3901 gccgggttca aacagtcacg ctatgccagt ggtgaatatt tcagccaata tctgcaaggt
     3961 aactggcagc cgaaaacggc gaaagttggc gaactgtttg cccgcagcgg tattacgtta
     4021 cctacccgtg agatgtgggc gcagctgcgc gatgacgtga tgcgctacgg catttacaac
     4081 cagaatctcc aggcggtgcc gccaaccggt tctatctctt atatcaacca tgctacgtcg
     4141 agtattcatc cgattgtggc gaaagtagag atacgcaaag agggcaaaac aggacgcgtt
     4201 tactaccctg ccccgtttat gactaacgaa aatctggcgc tatatcagga cgcttacgaa
     4261 attggcgcag aaaagatcat cgacacctac gcggaagcga ctcgccatgt cgatcagggg
     4321 ctgtcgctga cgctgttttt ccccgatacc gccaccactc gcgatatcaa caaagcgcag
     4381 atttacgcct ggcgcaaagg tatcaaaacg ctctattaca ttcgcctgcg tcagatggcg
     4441 ctggaaggca ctgaaattga aggctgcgtc tcctgtgcac tttaaggaat atctatgaaa
     4501 ctctcacgta tcagcgccat caactggaac aagatatctg acgataaaga tctggaggtg
     4561 tggaatcgcc tgaccagcaa tttctggcta ccagaaaagg tgccgctgtc gaacgatatt
     4621 cctgcctggc agacattaac tgtcgtagaa caacaactga cgatgcgcgt ttttactggc
     4681 ctgacgctgc tcgacacgct gcaaaatgtt atcggcgcgc cttcgctgat gcccgatgca
     4741 ctcacgcctc atgaagaagc ggtattatcg aatatcagct ttatggaagc ggttcatgcc
     4801 cgctcttaca gttcgatttt ctcgacgctg tgccagacca aagatgtcga tgccgcctac
     4861 gcctggagtg aagaaaacgc accgttgcag cgaaaagctc agattattca gcaacattat
     4921 cgcggtgatg atccgctgaa aaagaaaatc gccagtgtgt ttcttgaatc ttttttgttc
     4981 tattccggtt tctggctgcc gatgtatttt tccagccgcg gaaagctgac caataccgca
     5041 gacctgatcc gtctgattat ccgcgatgaa gcagtccacg gttactacat aggctataaa
     5101 tatcagaaaa acatggaaaa gatatctctg ggacaacgtg aagagttgaa gagtttcgcc
     5161 ttcgatttgt tgctggaact ctacgacaac gagttgcaat acaccgatga gctgtacgcc
     5221 gaaaccctgt gggctgacga tgtgaaagcg tttctctgtt acaacgccaa taaggctttg
     5281 atgaatctgg gctacgaacc tttatttccc gcagaaatgg cggaagtgaa tccggcaatc
     5341 ctcgccgcgc tttcgccgaa tgccgatgaa aatcacgatt tcttttccgg ttcaggctcc
     5401 tcttatgtga tggggaaagc ggttgaaaca gaagatgaag actggaattt ctgagggtgt
     5461 tattttcaaa aatatcacta cccgcagcag ggaaataatt cccgccaaat agctttttat
     5521 cacgcaaata ttttgtggtg atctacactg atactctgtt gcattattcg cctgaaacta
     5581 caatattcag gcgttttttc gccatctatg acaaaaaata tcaacttttc tcgatttgct
     5641 ctcagccctt atatcacggg aaattccggc gatttgctcg catcaatatt catgccacat
     5701 ttgccctcag gggttgcctc agattctcag tatgttaggg tagaaaaaag tgactatttc
     5761 cattgggtaa tatatcgaca taaacaaata aaggaatctt tctattgcat ggcaattaaa
     5821 ttagaaatta aaaatcttta taaaatattt ggcgagcatc cacagcgagc gttcaaatat
     5881 atcgaacaag gactttcgaa agaacaaatt ctggaaaaaa ctgggctatc gcttggcgta
     5941 aaagacgcca gtctggccat tgaagaaggc gagatatttg tcatcatggg attatccggc
     6001 tcgggtaaat ccacaatggt acgccttctc aatcgcctga ttgaacccac ccgcgggcaa
     6061 gtgctgattg atggtgtgga tattgccaaa atatccgatg ccgaactccg tgaggtgcgc
     6121 agaaaaaaga ttgcgatggt cttccagtcc tttgccttaa tgccgcatat gaccgtgctg
     6181 gacaatactg cgttcggtat ggaattggcc ggaattaatg ccgaagaacg ccgggaaaaa
     6241 gcccttgatg cactgcgtca ggtcgggctg gaaaattatg cccacagcta cccggatgaa
     6301 ctctctggcg ggatgcgtca acgtgtggga ttagcccgcg cgttagcgat taatccggat
     6361 atattattaa tggacgaagc cttctcggcg ctcgatccat taattcgcac cgagatgcag
     6421 gatgagctgg taaaattaca ggcgaaacat cagcgcacca ttgtctttat ttcccacgat
     6481 ctcgatgagg ctatgcgtat tggcgaccga attgccatta tgcaaaatgg tgaagtggta
     6541 caggtcggca caccggatga aattctcaat aatccggcga atgattatgt ccgtaccttc
     6601 ttccgtggcg ttgatattag tcaggtattc agtgcgaaag atattgcccg ccggacaccg
     6661 aatggcttaa ttcgtaaaac ccctggcttc ggcccacgtt cggcactgaa attattgcag
     6721 gatgaagatc gcgaatatgg ctacgttatc gaacgcggta ataagtttgt cggcgcagtt
     6781 tccatcgatt cgcttaaaac cgcgttaacg cagcagcaag gtcttgatgc ggcgctgatt
     6841 gatgcgccgt tagcagtcga tgcacaaacg cctcttagcg agttgctctc tcatgtcgga
     6901 caggcaccct gtgcggtgcc cgtggtcgac gaggaccaac agtatgtcgg catcatttcg
     6961 aaaggaatgc tgctgcgcgc tttagatcgt gagggggtaa ataatggctg atcaaaataa
     7021 tccgtgggat accacgccag cggcggacag tgccgtgcaa tccgcagacg cctggggtac
     7081 accggcgact gcaccgactg acggtggtgg tgctgactgg ctgaccagta cgcctgcgcc
     7141 aaacgtcgag cattttaata ttctcgatcc gttccataaa acgctgatcc cgctcgacag
     7201 ttgggtcact gaagggatcg actgggttgt tacccatttc cgtcccgtct tccagggcgt
     7261 gcgcgttccg gttgattata tcctcaacgg tttccagcaa ttgctgctgg gtatgcccgc
     7321 accggtggcg attatcgttt tcgctctcat cgcctggcag atttccgggg tcggaatggg
     7381 tgtggcgacg ctggtttcgc tgattgccat cggcgcaatc ggtgcctggt cgcaggcaat
     7441 ggtgactctg gcgctggtgt taaccgccct gctgttctgt atcgtcatcg gtttgccgtt
     7501 ggggatctgg ctggcgagaa gtccacgagc ggcgaaaatt attcgtccac tgcttgatgc
     7561 catgcagacc acgccagcgt ttgtttatct ggtgccaatc gtcatgctgt ttggtatcgg
     7621 taacgtgccg ggcgtggtgg tgacgatcat ctttgctctg ccgccgatta tccgtctgac
     7681 cattctgggg attaaccagg ttccggcgga tctgattgaa gcctcgcgct cattcggtgc
     7741 cagcccgcgc cagatgctgt tcaaagttca gttaccgctg gcgatgccga ccattatggc
     7801 gggcgttaac cagacgctga tgctggccct ttctatggtg gtcattgcct cgatgattgc
     7861 cgtcggcggg ctgggtcaga tggtacttcg cggtatcggt cgtctggata tggggcttgc
     7921 caccgttggc ggcgtcggga ttgtgatcct cgccattatc ctcgatcgcc tgacgcaggc
     7981 cgttgggcgc gactcacgca gtcgcggcaa ccgtcgctgg tacaccactg gccctgtcgg
     8041 tctgctgacc cgcccattca ttaagtaact ctgcacttgc ccggtgacgc cgggcatatc
     8101 accctgccaa aaaaaggaat aacaatgcga catagcgtac tttttgcgac agcgtttgcc
     8161 acgcttatct ctacacaaac ttttgctgcc gatctgccgg gcaaaggcat tactgttaat
     8221 ccagttcaaa gcaccatcac cgaagaaacc ttccagacac tgctggtcag tcgtgcgctg
     8281 gagaaattag gttataccgt caataaaccc agcgaagtgg attacaacgt tggctacacc
     8341 tcgcttgctt ccggcgatgc aaccttcacc gccgtgaact ggacgccact gcatgacaac
     8401 atgtacgaag ctgccggtgg cgataagaaa ttttatcgtg aaggggtatt tgttaacggc
     8461 gcggcacagg gttacctgat cgataagaaa accgccgacc agtacaaaat caccaacatc
     8521 gcacaactga aagatccgaa gatcgccaaa ctgttcgata ccaacggcga cggtaaagcg
     8581 gatttgactg gctgtaaccc tggctggggc tgcgaaggtg cgatcaacca ccagcttgcc
     8641 gcgtatgaac tgaccaatac cgtgacgcat aatcagggga actacgcggc gatgatggct
     8701 gacaccatca gtcgttacaa agagggcaaa ccggtgtttt actacacctg gacgccgtac
     8761 tgggtgagta atgagctgaa gccagggaaa gatgtggtct ggttgcaggt gccgttctct
     8821 gcactgccgg gcgataaaaa tgccgatacc aaactgccga atggcgcgaa ttacggcttc
     8881 ccggtcagca ccatgcatat cgttgccaat aaagcctggg ccgagaaaaa cccggcagca
     8941 gcgaaactgt ttgccattat gcagttgcca gtggcagata ttaacgccca gaacgccatt
     9001 atgcatgacg gcaaagcctc agaaggcgat attcagggcc atgttgatgg ctggatcaaa
     9061 gcccaccagc agcagttcga tggctgggtg aatgaggcgc tggcagcgca gaagtaattt
     9121 ttattcgggc ggattaggcg tttttgcctc atccgccgct ctgtacacaa tgcctgatgc
     9181 gacgctaacg cgtcctatca tgcctacaaa ccacatcgca catttcaaca atctatcttt
     9241 catcccatat tcatcaacat ccgctattat tgatttccag cttaatcatc acctgatgaa
     9301 caaaaataat gaccaaacct aatcatgagc ttagcccggc gctgatcgtg ctgatgtcta
     9361 tcgccaccgg tctggcggta gccagtaatt attacgccca gccattgctc gacaccatcg
     9421 cgcgtaactt ttccctttcc gccagttcgg caggctttat tgttaccgcc gcgcagttgg
     9481 gctatgccgc aggtctactg tttcttgttc ccctcggtga tatgtttgaa cgccgccgcc
     9541 tgattgtctc gatgacctta ctggcggcag gcggcatgtt aatcactgcc agcagtcagt
     9601 cgctggcgat gatgatcctc ggtacggcat taaccggttt attctcagtc gtggcacaaa
     9661 ttctggttcc actggcagcg acgctggctt caccggacaa acgcggcaaa gtggttggca
     9721 ctattatgag cggtctgctg ttggggatct tactcgcacg gaccgttgcc ggattgctgg
     9781 cgaatctcgg cggctggcgc accgtctttt gggttgcctc ggtgttaatg gcactgatgg
     9841 cgctggcatt atggcgtggt ctgccacaaa tgaaatcaga aacccacctc aactacccac
     9901 agttgttggg ttccgttttc agtatgttta tcagcgataa aattctgcgt acccgcgcgt
     9961 tgctgggctg cctgaccttt gccaatttca gcattctctg gacctcaatg gcctttttgc
    10021 ttgccgctcc accttttaac tacagcgatg gtgtaattgg tctgtttgga cttgcgggag
    10081 ctgccggagc gttgggcgct cgtccggcgg gcggttttgc cgataagggc aaatcgcacc
    10141 acaccacaac tttcggtctg ctgctgctat tactttcatg gctggcgatc tggtttggtc
    10201 acacttccgt actggcgttg attatcggaa tcctggtgct ggatctcacc gttcagggcg
    10261 tgcatatcac taaccagacg gtaatttatc gaatacaccc tgatgcgcgt aatcgcctga
    10321 ccgcaggtta catgaccagc tactttattg gcggtgccgc cggttcgcta atttcagcct
    10381 cagcctggca acatggcggt tgggctggcg tttgtctggc tggcgcgacg attgccctgg
    10441 ttaacttact ggtctggtgg cgaggttttc atcgtcagga agccgcaaat taagcaatca
    10501 gcaaccgttt tcgtggcttt acacttataa gggtgttaag aagcccatca gtctgataag
    10561 gttaagatat tcattcagtc tatttataat attaacaatc gttaagcgta cactctatgg
    10621 aaagccctac tccacagcct gctcctggtt cggcgacctt catggaagga tgcaaagaca
    10681 gtttaccgat tgttattagt tatattccgg tggcctttgc gttcggtctg aatgcgaccc
    10741 gtctgggatt ctctcctctc gaaagcgttt ttttctcctg catcatttat gcaggcgcga
    10801 gccagttcgt cattaccgcg atgctggcag cagggagtag tttgtgggtt gctgcactga
    10861 ccgtcatggc aatggatgtt cgccatgttt tgtatggccc gtcactgcgt agccgtatta
    10921 ttcagcgtct gcaaaaatcg aaaaccgccc tatgggcgtt tggcctgacg gatgaggttt
    10981 ttgccgccgc caccgcaaaa ctggtacgca ataatcgccg ctggagcgag aactggatga
    11041 tcggcattgc cttcagttca tggtcatcgt gggtatttgg tacggtaata ggggcattct
    11101 ccggcagcgg cttgctgcaa ggttatcccg ccgttgaagc agcattaggt tttatgcttc
    11161 cggcactctt tatgagtttc ctgctcgcct ctttccagcg caaacaatct ctttgcgtta
    11221 ccgcagcgtt agttggtgcc cttgcaggcg taacgctatt ttctattccc gtcgccattc
    11281 tggcaggcat tgtctgtggc tgcctcactg cgttaatcca ggcattctgg caaggagcgc
    11341 ccgatgagct atgaggttct gctgcttggg ttactggttg gcgcagcgaa ttattgcttc
    11401 cgctatttgc cgctgcgcct gcgtgtgggt aatgcccgcc caaccaaacg tggcgcggta
    11461 ggtattttgc tcgacaccat tggcatcgcc tcgatatgcg ctctgctggt tgtctctacc
    11521 gcaccagaag tgatgcacga tacacgccgt ttcgtgccca cgctggtcgg cttcgcggta
    11581 ctgggtgcca gtttctataa aacacgcagc attatcatcc caacactgct tagtgcgctg
    11641 gcctatgggc tcgcctggaa agtgatggcg attatataac tcacaagaat catttttcta
    11701 aaacaataca tttactttat ttgtcactgt cgttactata tcggctgaaa ttaatgaggt
    11761 catacccaaa tggatagttc gtttacgccc attgaacaaa tgctaaaatt tcgcgccagc
    11821 cgccacgaag attttcctta tcaggagatc cttctgactc gtctttgcat gcacatgcaa
    11881 agcaagctgc tggagaaccg caataaaatg ctgaaggctc aggggattaa cgagacgttg
    11941 tttatggcgt tgattacgct ggagtctcag gaaaaccaca gtattcagcc ttctgaattg
    12001 agttgtgctc ttggatcatc ccgtaccaac gcgacgcgta ttgccgatga actggaaaaa
    12061 cgcggttgga tcgaacgtcg tgaaagcgat aacgatcgcc gctgcctgca tctgcaatta
    12121 acggaaaaag gtcacgagtt tttgcgcgag gttttaccgc cgcagcataa ctgcctgcat
    12181 caactctggt ccgcgctcag cacaacagaa aaagatcagc tcgagcaaat cacccgcaaa
    12241 ttgctctccc gtctcgacca gatggaacaa gacggtgtgg ttctcgaagc gatgagctaa
    12301 cgcgtcatct cgctcaaaaa tccagattta taaaagaaaa aatgaccggc cagcatcgca
    12361 acatgctggc ctttttggca agcaggtcgg ctcagccgat gagttaagaa gatcgtggag
    12421 aacaatatga gcgcaaatgc ggagactcaa accccgcagc aaccggtaaa gaagagcggc
    12481 aaacgtaagc gtctgctcct ccttctcacc ttgctcttta taattattgc cgtagcgata
    12541 gggatttatt ggtttttggt actgcgtcat ttcgaagaaa ccgatgacgc atacgtggca
    12601 gggaatcaga ttcaaattat gtctcaggtg tctggcagcg tgacgaaagt ctgggccgat
    12661 aacaccgatt ttgtaaaaga aggcgatgtt ctggtcactc tcgacccgac agatgctcgc
    12721 caggcgtttg aaaaagccca aactgcactg gcttccagcg ttcgccaaac ccaccagctg
    12781 atgattaaca gcaagcagtt gcaggcgaat attgaggtgc agaaaatcgc cctcgcgaaa
    12841 gcacaaagcg actacaaccg ccgtgtgccg ctgggcaatg ccaacctgat tggtcgcgaa
    12901 gagctgcaac acgcccgcga cgccgtcacc agtgcccagg cgcaactgga cgtcgctatt
    12961 caacaataca atgccaatca ggcgatgatt ctggggacta aactggaaga tcagcctgcc
    13021 gtgcaacagg ctgccaccga agtacgtaac gcctggctgg cgctggagcg tactcgtatt
    13081 gtcagtccga tgacaggtta tgtctcccgt cgtgccgtgc aaccgggtgc gcaaattagc
    13141 ccaacgacgc cgctgatggc ggtcgttcca gccaccaata tgtgggtgga tgccaacttt
    13201 aaagagacgc agattgccaa tatgcgtatc ggtcagccgg tcactatcac cacggatatt
    13261 tacggcgatg atgtgaaata caccggtaaa gtggttggtc tggatatggg cacaggtagc
    13321 gcattctcac tgcttccggc gcaaaatgcg accggtaact ggatcaaagt cgttcagcgt
    13381 ctgcctgtgc gtatcgaact tgaccagaaa cagctggagc aatacccgct gcgtatcggt
    13441 ttatccacgc tggtgagcgt caataccact aaccgtgacg gtcaggtact ggcaaataaa
    13501 gtacgttcca ctccggtagc ggtaagcacc gcgcgtgaaa tcagcctggc acctgtcaat
    13561 aaactgatcg acgatatcgt aaaagctaac gctggctaat ccagaggtgc gtgtgatgca
    13621 acagcaaaaa ccgctggaag gcgcgcaact ggtcattatg acgattgcgc tgtcactggc
    13681 gacattcatg caggtgctgg actccaccat tgctaacgtg gcgatcccca ctatcgccgg
    13741 gaatctgggc tcatcgctca gccagggaac gtgggtaatc acttctttcg gggtggcgaa
    13801 tgccatctcg atcccgctta ccggctggct ggcaaagcgc gtcggggaag tgaaactgtt
    13861 cctctggtcc accatcgcct ttgctattgc gtcgtgggcg tgtggtgtct ccagcagcct
    13921 gaatatgctg atcttcttcc gcgtgattca ggggattgtc gccgggccgt taattccgct
    13981 ttcgcaaagt ctattgctga ataactaccc gccagccaaa cgctcaatcg cgctggcgtt
    14041 gtggtcgatg acggtgattg tcgcgccaat ttgcggccca atcctcggag ggtatatcag
    14101 cgataattac cactggggct ggatattctt catcaacgtg ccgattggcg tggcggtggt
    14161 gttgatgacc ctgcaaaccc tgcgcggacg tgaaacccgc accgaacggc ggcggattga
    14221 tgccgtgggg ctggcactgc tggttattgg tataggcagc ctgcagatta tgctcgaccg
    14281 cggtaaagag ctggactggt tttcatcaca ggaaattatc atccttaccg tggtggcggt
    14341 ggtggctatc tgcttcctga ttgtctggga gctgaccgac gataacccga tagtcgatct
    14401 gtcgttgttt aagtcgcgca acttcaccat cggctgcttg tgtatcagcc tcgcgtatat
    14461 gctctacttc ggcgctattg ttctgctgcc gcagttgttg caggaggtct acggttacac
    14521 ggcaacgtgg gcaggtctgg cctctgcgcc ggtagggatt attccggtga tcctgtcgcc
    14581 gattatcggc cgcttcgcgc ataaactgga tatgcggcgg ctggtaacct tcagctttat
    14641 tatgtatgcc gtctgcttct actggcgtgc ctataccttt gaaccaggta tggattttgg
    14701 cgcgtcggcc tggccgcagt ttatccaggg gtttgcggtg gcctgcttct ttatgccgct
    14761 gaccactatt acgctgtctg gtttgccacc ggaacgactg gcggcggcat cgagcctctc
    14821 caactttacg cgtacgctgg cggggtctat cggcacgtcg ataaccacaa ccatgtggac
    14881 caaccgcgag tcgatgcacc atgcgcagtt aaccgagtcg gtaaacccgt ttaacccaaa
    14941 cgcccaggcg atgtacagtc aactggaagg gcttgggatg acgcaacagc aggcatcagg
    15001 ctggattgcc cagcagatca ccaatcaggg gctgattatt tccgccaatg agatcttctg
    15061 gatgtcagcc gggatattcc tcgtcttgct ggggctggtg tggtttgcta aaccgccctt
    15121 tggcgcaggt ggcggcggag gcggtgcgca ctaaggttgt caaagggagc tgcggctccc
    15181 tttttatttg ctcattctgt aaacggttgt tttatcctct cattcggtgt atcttttcgc
    15241 cgccccaaac tcttccattc accattaacc ggttctgagc attcgctcag gttgttaatt
    15301 ctctttttcc ttcgccctcg ctttcgcggg tgatactcac atttttcttt taaccccata
    15361 actcgcaccc tcgcgggcgt acatttatta cacacaaaat aatggattat tatgaatacc
    15421 caacttaaat acgggagaac ctggcattat cctttctccc ccggcaccac cagcgatgac
    15481 cgcattaacg ccgattactg gcaggattta caggccatca cgcaattggt gcacacagaa
    15541 aaactcgacg gcgaaaataa ctgccttaac cgctacggcg tgtttgcccg ttcccatgct
    15601 gcgcctacgc agtcggcatg gacttataaa attcgccagc gttggcaact gctgaaaaac
    15661 gatctcggcg atctggaact gtttggtgaa aatctctacg ccgttcattc cattgaatac
    15721 cgcgcactgg aacaggactt ttacctgttt gccgttcgct gccaggatat gtggctgagc
    15781 tgggaagagg tgcagtttta cgccgctctg tttgatttcc cctgcgttcc cgaaatctcc
    15841 ggtccgcagc cggggaatga tgaaaaaagc tggcaacgtg attttctggc actgaccaat
    15901 gcgcgcggcg cgttcgatcc ctgggataca caaaccgggc aaccctgtac gctggaaggc
    15961 attgtcagcc ggaatcgtga tgcgttttcc gtcgctgact tttcgcataa cgtcttcaag
    16021 tatgtgcgca aaaatcatgt caaaaccacc gtgcactgga aacgacactg gcaacgggcg
    16081 cggatggccc atgagttcgc ttatggagaa cagtcatgat ttggcagctt accgacgata
    16141 aacgctggtc ggcgctacgt cagcgtttta gctgggttga ggagatgcat cacacgccac
    16201 aagaccctga gcatcatggt gaaggcgacg tcggcgtgca taccgaaatg gtgctgaacg
    16261 ccctcgttac tctgccagaa ttccagcaac tccccgctca acagcaggaa gttttatggg
    16321 cggcggcgtt attgcatgat gttgaaaagc gcagcactac cgtgcaggaa aacggacgta
    16381 ttcagtcgcc gggccatgct cgtcggggcg aactgacggc ccggctgatt ttgtggcgcg
    16441 atatcccaac gccgttcgtg ctgcgcgagc aaatcgttgc gctggtgcgt ctgcatgggt
    16501 taccgctatg gttactggaa cgtccggaac cggagcgttt gttgctcacg gctgcaatgc
    16561 gcatcgacac gcgtctgctc gctttgcttg ctcgtgccga tctgcttggt cgccaaagcc
    16621 cggatcggca atcaatgctg gaacgcatcg atctgttcga gctgttttgc caggagcaac
    16681 agtgttgggg aaaagtgcgc tcgttcgtgt ctgattccgc acgctggcat tacctgaccc
    16741 atgagcaaaa ttctcctgat tttgttccct gggaggctga accttttgag gtcattttgc
    16801 tgtgtggctt accgggtatg ggcaaagatc gctatatcag taaacagtga cagggaattg
    16861 acgttatcag ccttgatgat atgcggcggc gaatcaatgc cagcccggat gacaaaaccg
    16921 ctaccgggcg tattgtgcaa caggcaaaag aagaggcgcg cgttttttta cgccagaaaa
    16981 agccgttcat ctggaatgca acgaacatta cccgccagct gcgtagccag ctcatcagcc
    17041 tgtttacggc ttacggcgca cgagtaaaaa tcgtttatct tgaagtgcca tgggcgcaat
    17101 ggaagcagca aaatgccaga cgcgagtatg cagttcctaa cgcggtcgtg atgcggatgg
    17161 cctcaaggct ggaagttcct caacctgatg aagcacatag cgtggagtat cgggtgattg
    17221 aaaggtaaaa aaaccggacg gtggtgtcac ctgcccggct tcttttgttc cactgactaa
    17281 atgtgcagtt cctgcaactt ctctttcggc agcgccagtt cttcgttgct gttgatgcgc
    17341 acgtcacgtt ccagaatgct acgcgcaata tcctgcgctt cctgcaacga gtgcatctgg
    17401 taagtgccac actggtagac gttcagctcc ggaatctgat tctgatcctg cactttcagc
    17461 acgtcttcca ttgccgcttt ccaggcatca gcaacacgct gctcatctgg cgtaccaatc
    17521 agactcatat aaaaaccggt gcggcagccc attggcgaga tatcgataat ctctacacca
    17581 ttaccgttaa gatggttacg cataaaacca gcaaacaggt gctccagggt atggatccct
    17641 ctttctggca tcacttcttt gttcggcacg cagaagcgca gatcgaacac ggtgattgcg
    17701 tcgccatgcg gggtgttcat tgttttcgcc acccgaactg caggcgcttc catccgggta
    17761 tgatcgactg tgaagctatc taacaacggc atttagccac ctccggtaat ttttttaaaa
    17821 attttctgaa ctctttcttc ccaggcgagt ctgagtatat gaaagacgcg catttgttat
    17881 catcatccct gaattcagag atgaaatttt ggccactcac gagtggcctt tttcttttct
    17941 gtcaggcgtg tttttccagc cacaccgcaa acggttcggt atctgcggct tccatttcct
    18001 gctgacggcg ttcagatgcc tcgcgctcgg ctacaaaatc ctcttcgcgc agaatttcca
    18061 gcggctcttc acgcagcaga ttacggtacg cttcagcaaa cgctttgcct gttccgccaa
    18121 taccagtatc aatcatagac cttaagatac gggcagagaa agtcagatcg ggattatcga
    18181 agcaggcgac cagttcatca cacactttct gatacgcttc gccgccgtta atgctatcca
    18241 gcgtttgcgc gacgcgtttc agatcgcgga acagatcttt acccacctgc ggtaacggga
    18301 actgcgcggt ttcgcagccg atacccagcg tcagacccgg tttgcgacct tcgaggatca
    18361 cccggttcca gttaacgcgt gtacaggcaa gttcgctact gctcatttcc ggtgcatcag
    18421 ccagcgcaca ccagaccata aacaggtcga ggaatcgcac ctgctgttca tctacaccaa
    18481 tcggcgagaa cgggttgatg tccagcgaac gcacttcaat atattcaatg ccgccacgta
    18541 acagcgcatc agaaggcgac tcgccgctgc gggtaacgcg ttttggacga atcggcgcgt
    18601 acagttcgtt ttcaatctgc agcacgttgc tgttgatttg cagcctctta ccgtctttct
    18661 caataccaat cttcgcgtac tcttccgatg gcgttttgat tgcctgttta aggcccgcta
    18721 cgtactcgta aagatcgttg aaggtaatac caagattgct ttgcgattta ttggtatagc
    18781 cgagatcgct caaacgaaga gaggtcgcat acggcaggta atacataccg cactcggttt
    18841 tctcaaacgg cagcgatgtt ggttttcctt gcaggaaaga agaacaaatc gccggagacg
    18901 cgccaaacag ataaggaatg acccaaccga aacgatagta attgcggata acgcggaaat
    18961 agcccgcaga aattttctct ttgccatcag cgcccgagat atcaccacac ttcgcttgcc
    19021 agaatgccat tggcaaagag aaattgtagt gcacgccgga aatggtttgc atcagcgcgc
    19081 catagcgatt tttcagccct tcacgataca gcgttttaaa gcgtccggtg ttagaagtgc
    19141 cgtactgtgc cagttcgatg tcctgacctt ctgcgatgta gcatggcata cttaacggcc
    19201 acatccgctc atcgcccata ttgcgcgccg tataacgatg cagatcgcgc ataaaggtca
    19261 gcatatgttc aatatcacca tccactggtg taatgaattc cagcaatgct tccgcaaaat
    19321 cggtagtaat ccatttgtgc gtcagtgcgg aacctaatgc ttcaggatga cctgttgttg
    19381 ccagtgtgcc atcagcatta acacgcaaag tttcgcgctc cagcccacgc tgtatcccct
    19441 ttaacgcctg aggatgtttt tccagccagg ccagcgcctg tgatacgtcc gggatcaaat
    19501 tgacctcccg cctgtcaaaa tcgttttaat tagcataact gtaatggtga ccatatgtgc
    19561 aggcctacaa ttagtgccac cacatcatgc cctgaacggt cgctgctgca actgcaacat
    19621 agcgtaacgc tttaccaagg cacaaaaaaa agattaccgg cccccaggag atgcgcatcc
    19681 atcccgctaa cagacacagt aaatcgccaa ccacgggcat ccagcttaat aatagcgtga
    19741 ctgcaccata gcgtttcagc cagccggtag ctttctcttg ccagcgcgat gttttacgca
    19801 atggaaagaa acgcccaagg ataacgttag ttaaccctcc aaggctatta cccattgttg
    19861 ctgttaaaac taaaacccag ggatgactga tcccagaaag caacattgcc accagcacga
    19921 cttcggagtt gccgggtaat agtgtagcgc tgagaaaact actggcaaac aacgagaaaa
    19981 gcgataacgc ttcactcaca gcaagcgaac atccacagcg tccatgcctg ctgcacgggc
    20041 cgcctgaata ccgaaatcgg catcttcaaa gaccacacac tgcgtcggtt gcacgcccat
    20101 acgctgcgcg cacaacaaaa atgtgtctgg cgcgggttta tggtgtttga cgtgatcggc
    20161 agcgacgacg gcgtcaaaat aacggcgtaa tcccaggtga gccagcaatg cctcagcgat
    20221 ggcgctttca ctccccgttc ctaccgccat tgggcgacga ccatgccaac ttttcaccac
    20281 ttcaacaaga ggaagcggct cgacactatc cagcagcata cttcttactg cttctgtttt
    20341 ttcacgcgct aacgcatgcg ggtcgagatc ggcctgattc agctcaataa ttgcctgagc
    20401 aatacgccag gtgggcgatc cattaagcgc aatcatcgcc tgaacatcgt actgaagacc
    20461 gtagtgccct aatacttcgc gccacgcttt acggtgcgta ggctccgtat ccaggattgt
    20521 gccatccata tcaaaaatta aacctgcata acgctcgtac atggtcttct cgcaagtcga
    20581 aaaatatgac gctactttag cgtaattgct tgattttgtc gctgatggcg aaggagaaga
    20641 aaattcagga aatggataaa gtagttattt agaaatgaga tatttttgaa ggaaattttt
    20701 tggagaagat ggtgcatccg ggaggattac tcgccttgcg gctcgccctt ctggccgttg
    20761 ctaaagcaac gttatcctcc ctggtgcctg cggttaatac cgctacctct tcgatatctt
    20821 cattgctgaa gatttcggag aa
//
LOCUS       DAPGOF010000083        20369 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.083, whole genome shotgun sequence.
ACCESSION   DAPGOF010000083 DAPGOF010000000
VERSION     DAPGOF010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.083"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            31..1485
                     /locus_tag="RHV13_002642"
     CDS             31..1485
                     /locus_tag="RHV13_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HDV1242164.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGLGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPLMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
     gene            1545..2906
                     /gene="allB"
                     /locus_tag="RHV13_002643"
     CDS             1545..2906
                     /gene="allB"
                     /locus_tag="RHV13_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HDV1242165.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGG
                     IAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            2963..4264
                     /locus_tag="RHV13_002644"
     CDS             2963..4264
                     /locus_tag="RHV13_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HDV1242166.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            4287..5432
                     /gene="glxK"
                     /locus_tag="RHV13_002645"
     CDS             4287..5432
                     /gene="glxK"
                     /locus_tag="RHV13_002645"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HDV1242167.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEACGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNDIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LAANGGNLARVASIEMEECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGEINLFNSARNIACAIKIGQGIKN"
     gene            complement(5660..6445)
                     /gene="allE"
                     /locus_tag="RHV13_002646"
     CDS             complement(5660..6445)
                     /gene="allE"
                     /locus_tag="RHV13_002646"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HDV1242168.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(6456..7691)
                     /gene="allC"
                     /locus_tag="RHV13_002647"
     CDS             complement(6456..7691)
                     /gene="allC"
                     /locus_tag="RHV13_002647"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HDV1242169.1"
                     /translation="MITHFRQAIEEALPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMGASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPDAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKDL
                     VATLTELCESEKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            complement(7713..8762)
                     /gene="allD"
                     /locus_tag="RHV13_002648"
     CDS             complement(7713..8762)
                     /gene="allD"
                     /locus_tag="RHV13_002648"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HDV1242170.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHVVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPSLNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            9079..10746
                     /locus_tag="RHV13_002649"
     CDS             9079..10746
                     /locus_tag="RHV13_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HDV1242171.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQAL
                     TQVRRWDSACQKLPDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAITPASSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDAHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPTLRNQLIADLGAKPSVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDSQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSPAAQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            10756..12015
                     /locus_tag="RHV13_002650"
     CDS             10756..12015
                     /locus_tag="RHV13_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HDV1242172.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELHPGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAVMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPSWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            12026..12841
                     /locus_tag="RHV13_002651"
     CDS             12026..12841
                     /locus_tag="RHV13_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HDV1242173.1"
                     /translation="MTIIHPLLASSSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLVAAWMQRAEETGLFGPLVLAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            12838..13731
                     /locus_tag="RHV13_002652"
     CDS             12838..13731
                     /locus_tag="RHV13_002652"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDV1242174.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(13868..14935)
                     /gene="purK"
                     /locus_tag="RHV13_002653"
     CDS             complement(13868..14935)
                     /gene="purK"
                     /locus_tag="RHV13_002653"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDV1242175.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRADETEQLPEECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITALPLPQPIV
                     NSPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVMWAQSKFS"
     gene            complement(14932..15441)
                     /gene="purE"
                     /locus_tag="RHV13_002654"
     CDS             complement(14932..15441)
                     /gene="purE"
                     /locus_tag="RHV13_002654"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDV1242176.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            complement(15559..16281)
                     /gene="lpxH"
                     /locus_tag="RHV13_002655"
     CDS             complement(15559..16281)
                     /gene="lpxH"
                     /locus_tag="RHV13_002655"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HDV1242177.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRTKVHKPWLQTLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            complement(16284..16778)
                     /gene="ppiB"
                     /locus_tag="RHV13_002656"
     CDS             complement(16284..16778)
                     /gene="ppiB"
                     /locus_tag="RHV13_002656"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HDV1242178.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            16952..18337
                     /gene="cysS"
                     /locus_tag="RHV13_002657"
     CDS             16952..18337
                     /gene="cysS"
                     /locus_tag="RHV13_002657"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDV1242179.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKSVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            complement(18373..18894)
                     /locus_tag="RHV13_002658"
     CDS             complement(18373..18894)
                     /locus_tag="RHV13_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDV1242180.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGIAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(19002..19214)
                     /gene="ybcJ"
                     /locus_tag="RHV13_002659"
     CDS             complement(19002..19214)
                     /gene="ybcJ"
                     /locus_tag="RHV13_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HDV1242181.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            complement(19216..20082)
                     /gene="folD"
                     /locus_tag="RHV13_002660"
     CDS             complement(19216..20082)
                     /gene="folD"
                     /locus_tag="RHV13_002660"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HDV1242182.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
BASE COUNT         4650 a         5166 c         5590 g         4963 t
ORIGIN      
        1 ttattcgata cgtgaaatta agagggattt atggaacatc agagaaaact atttcagcaa
       61 cgcggctata gcgaagatct attgccgaaa acgcaaagcc agcggacctg gaaaacattt
      121 aactacttta ccttatggat gggttcggtt cataacgttc ccaattatgt gatggtcggc
      181 ggctttttta ttctcggctt gtctaccttt agtattatgc tggcaattat cctcagcgcc
      241 tttttcattg ccgcggtaat ggtattaaac ggtgctgcgg gcagtaaata cggcgtaccg
      301 tttgccatga tcctgcgtgc ttcttacggc gtacgtggcg cactgtttcc cggattatta
      361 agaggcggta ttgctgccat tatgtggttt ggcctgcaat gttacgcagg gtcactggcc
      421 tgcttgattc tgattggcaa aatctggccg ggatttttga ctctcggtgg tgatttcact
      481 ctgttaggcc tttctctacc gggcttaatt actttcttac tcttctggct ggtcaacgtt
      541 ggtataggtt ttggcggtgg caaagtttta aataaattca ctgccattct taacccgtgc
      601 atctatatcg ttttcggcgg tatggcgatt tgggcgattt cgctggtcgg gctcggtcca
      661 atctttgact acattccgag cggtattcag aaagcagaaa acagtggatt cttgttcctg
      721 gtggtgatta acgcggtagt tgcggtctgg gcggcaccgg cggtgagcgc atccgacttt
      781 acgcaaaacg cccactcgtt tcgtgagcaa gcgctggggc aaacgctggg tttagttgtg
      841 gcctatattc tgtttgcggt cgccggggta tgtattattg ccggagccag tattcactac
      901 ggcgctgaca cctggaacgt gctggatatt gttcagcgtt gggacagcct gtttgcctcg
      961 ttctttgcgg tactggttat tctgatgacg accatttcca ctaacgccac cggtaatatt
     1021 attccggccg gttatcagat tgccgctatt gcaccgacga aactgaccta taaaaacggc
     1081 gtactgattg ccagtattat cagcctgctg atctgcccgt ggaaattaat ggaaaatcag
     1141 gacagtattt atctgttcct cgatatcatc ggcggaatgc tgggtccggt aattggtgtc
     1201 atgatggcgc attattttgt ggtgatgcgt gggcaaatta atcttgatga actgtacacc
     1261 gcaccaggcg attataaata ttacgataac ggttttaacc tcaccgcgtt ttcagtaact
     1321 ctggtcgccg ttattttatc tcttggcggt aagtttattc ccttaatgga accgttatcg
     1381 cgtgtttcat ggtttgtcgg cgtcatcgtc gcctttgcgg cctacgcctt attaaagaaa
     1441 cgtacaacag cagaaaaaac aggagagcaa aaaaccatag gttaattaat cccgatattg
     1501 aacattgagt taaaaaccaa tctgtatttt acaaggagtt tgttatgtct tttgatttaa
     1561 tcattaaaaa cggcaccgtt attttagaaa acgaagctcg cgttgtagat atcgccgtta
     1621 aaggcgggaa aattgctgct atcggtcagg atctgggcga tgcaaaagaa gttatggatg
     1681 cgtctggtct ggtggtttcg ccgggcatgg ttgatgcgca cacccatatt tctgaaccgg
     1741 gtcgtagcca ctgggaaggt tatgaaaccg gtactcgcgc agcggcaaaa ggtggtatca
     1801 ccaccatgat cgaaatgccg ctcaaccagc tgcctgcaac ggttgaccgc gcttcaattg
     1861 aactgaagtt cgatgccgct aaaggcaagc tgactatcga tgcggcacaa ctcggtggcc
     1921 tggtgtctta caacatcgac cgtctgcatg agctggatga agtgggcgtt gtcggcttca
     1981 aatgcttcgt tgcgacctgt ggcgatcgcg gtatcgacaa cgacttccgt gatgtaaacg
     2041 actggcagtt cttcaaaggc gcgcagaagc tgggcgaact ggggcagccg gtgctggtgc
     2101 actgcgaaaa cgcgctgatc tgtgacgcac tgggcgaaga agcgaaacgt gaaggtcgcg
     2161 taactgccca tgactatgtg gcttcgcgtc cggtatttac cgaagtggaa gcgattcgcc
     2221 gcgtgctgta tctggcgaaa gttgccggtt gccgtctgca cgtttgccat atcagcagcc
     2281 cggaaggtgt tgaggaagtg actcgtgcac gtcaggaagg tcaggacgtt acttgtgaat
     2341 cctgcccgca ttactttgta ctggataccg atcagttcga agaaatcggt actctggcga
     2401 agtgttcacc gccgatccgc gatctggaaa accagaaagg catgtgggaa aaactgttta
     2461 acggtgaaat cgactgcctg gtttccgacc actcaccatg ccctccggaa atgaaagccg
     2521 gcaacatcat ggaagcatgg ggcggtattg ccggtctgca aaactgtatg gacgtgatgt
     2581 tcgatgaagc ggtacagaaa cgcggaatgt ctctgccaat gttcggcaaa ttaatggcga
     2641 ctaacgcagc agatattttc ggtctgcagc aaaaaggccg tatcgcccca ggaaaagatg
     2701 ccgacttcgt cttcattcag ccgaatagca gctatgttct taccaatgac gatctggaat
     2761 atcgccacaa agtcagcccg tatgttggcc gtaccattgg cgcgcgtatc acgaaaacca
     2821 tcttacgtgg tgatgtgatt tacgacattg aacagggctt ccctgttgcg ccgaaaggtc
     2881 aatttatcct taaacatcag cagtaatctg gcccctgcaa tgcccgtcct tgcggcgggc
     2941 attctccggt taaggtgtgt ttatgttcaa ttttgcagtc agccgcgaaa gcctgttatc
     3001 aggatttcag tggtttttct ttattttttg caacacggtt gtggttcctc ctacgctact
     3061 ttctgctttt cagttgccgc aaagtagcct gcttacactc acacaatatg cttttcttgc
     3121 taccgcactg gcctgcttcg ctcaggcgtt ctgtggccat cgtcgcgcta ttatggaagg
     3181 gccggggggc ctgtggtggg gaaccatcct aactatcacc cttggcgaag catcgcgcgg
     3241 gacaccgatc aacgatatcg ctaccagtct ggcggtgggg attgcactct ccggcgtgct
     3301 gacgatgttg attggtttta gcggattagg ccatcgcctg gcacggttat ttacgccgtc
     3361 ggtgatggtc ttgtttatgt tgatgctggg cgcgcagctg accactatct ttttcaaagg
     3421 tatgctcggg ctgccgtttg gcatagccga cccgaatttt aaaattcagt taccgccgtt
     3481 cgcgctctcg gtggcggtga tgtgcctggt actggcgatg attattttcc tgccgcaacg
     3541 ttttgcccgt tatggcctgc tggtcggcac cataaccggc tggttgttgt ggtacttttg
     3601 ctttccttct tcgcattcgc tctccggtga gttgcactgg cagtggttcc cgctcggcag
     3661 tggcggtgct ttgtctccgg ggattattct gacggcggtg attacaggtc tggtgaatat
     3721 cagcaatacc tacggtgcga ttcggggcac ggatgtgttt tatccgcagc agggcgcggg
     3781 gaatacgcgt tatcgtcgca gctttgtggc gaccggattt atgacgctga taaccgtacc
     3841 gctggcggta attccatttt caccgtttgt ttcatccatt ggtttattaa cccagactgg
     3901 cgattacacg cggcgttcgt ttatttatgg cagcgttatt tgcctgctgg tggcgctggt
     3961 tcctgcactc acgcgactgt tttgcagtat ccctttaccc gtgagtagtg cggtcatgct
     4021 ggtttcttat ctgcctttac tcttttccgc gctggtgttt agccagcaaa taacgtttac
     4081 cgctcgcaat atttatcgac tcgcattgcc gttatttgtc ggcatatttt taatggcatt
     4141 accgcctgtg tatctgcaag accttccatt aacgcttcgt cctctgctca gtaacggctt
     4201 attggtcggg attttactgg ctgttcttat ggataacctt attccgtggg aacgcatcga
     4261 ataatttgtt gaaaaaagga ttgataatga agattgtcat tgcgccagac tcttttaaag
     4321 agagcttaag tgcagaaaaa tgttgtcagg caattaaagc cgggttttcg accctctttc
     4381 ccgatgcgaa ctatatctgt ttgccgatag cggatggcgg cgaagggacg gtggatgcga
     4441 tggtcgccgc gacgggtggt aacatcgtga cgcttgaagc ctgcgggccg atgggcgaaa
     4501 aagtgaatgc tttttatggc cttaccggcg acgggaaaac agcggtgatt gagatggcgg
     4561 cagcaagtgg cctgatgctg gtcgcgcctg aaaagcgtaa tccgttgctg gcctccagtt
     4621 ttggtacggg ggagttaatt cggcatgcgc tggataacga cattcgccat attattctcg
     4681 gcattggcgg cagtgcgacg gtcgacggcg gtatgggcat ggcgcaggcg cttggtgtgc
     4741 gttttctcga tgccgacggt caggtgctgg cagctaacgg cggcaattta gcgcgcgtgg
     4801 caagcattga gatggaggaa tgcgatccgc gtctggcgaa ctgccatatt gaagtagcat
     4861 gtgacgttga taacccgctg gtaggggcac gcggcgcggc ggcggtgttt ggcccgcaaa
     4921 aaggggcaac gccggagatg gtcgaagagc tggaacaggg gctgcaaaat tacgcccgtg
     4981 ttttacaaca gcaaactgaa attaatgtct gtcagatggc gggcggcggc gctgcgggcg
     5041 gtatgggtat tgcggcggcg gtatttctca atgcggatat taaaccaggt attgagattg
     5101 tgttgaatgc ggtcaatctt gcgcaggcag tgcagggcgc agcactggtg attaccgggg
     5161 aagggcgcat cgactcgcaa acggcaggcg gtaaagcgcc gctgggtgtg gcgtcggtgg
     5221 cgaagcagtt taatgtaccg gtgattggga ttgctggcgt attgggtgat ggcgtggaag
     5281 tggtgcacca gtatggcatt gatgcggtat tcagcatttt gccacgtctg gcacctttag
     5341 ccgaagtgct cgccagcggt gaaatcaatc tcttcaacag cgcgcgaaat attgcctgcg
     5401 ccattaaaat aggtcaggga attaaaaact gactcttgcc tttaaagcgg atgctattta
     5461 tttcgtataa gagcgcagta ctcatgccgg atgcggcatg agtactatat ccttcctgaa
     5521 aatcgcgcaa attctatata ttgcagagat catgtaggcc tgataagcga agcgcatcag
     5581 gcaatgttac aaaaaaagcc acggtataaa ccgtggcaaa atccaacata gctaaaaata
     5641 atcaggcgag tggtatgact taaatctcta cgtcgcggtt gcaatctttc gagtaaatat
     5701 agctgaacgc ttcaccacgc cctacaccat aaccagcctg taaagaataa gcgcccataa
     5761 agatgtaatc gccttttttc accgggatcc agttattgtc gaggttataa accccctgac
     5821 cggaaagaat ataggcaccg tgttcctgaa catgtgtttc gatataaccg tggctggcac
     5881 ctggtgcaaa agagaggata tgcatgttca tatcaaaacc taactctttg ggcagaaaat
     5941 ccagcaggat cacatcatcc ataccttcat agtgaatgcg ttccagttcg ctggcattgc
     6001 cagaaaccag ccacggtgca tggccttcta ccggaacata gcggcgctta tataaaaaga
     6061 tttggctgtc ttcggcctgg gcgttaacaa acgtcattaa ggagcctggt gggcaataaa
     6121 gatagccacc ttcgcttaag gcaaatgttt tgccttcggc tttggcagtg atatttccag
     6181 agatcacata caggaacgtt tcaatgcctt cgccaccgaa gccctgttgg ttgccaccgt
     6241 tttgatgcag tgtgaccaga taatcaacaa aagaggcacc cagctttggc gtggagagga
     6301 ttgtcgcgtc acaattttca aagcccggaa taatattttt taccagaccg tctggtgtta
     6361 acagtgcgaa attaccgtgt ttcacaatcg cacggttagc cagtaaatca tcgcggtaac
     6421 cggtgacgtt atttaaatat cccatttatg actccttatt tctgccaggc aagttgataa
     6481 agcatgagtg ccaacgtttt gaccccttcg gcaaggtcgg taatattggt gcgttccgcc
     6541 gggttatggc tgatcccgtt gatgctggga atgaaaatca tgcaggtcgg cacgcgaggc
     6601 gcgaaaattt gcgcgtcgtg cccggcacca ctgtgcatca cccggtaatt cagtttttca
     6661 ctttcacaca attctgtcag ggtggcgacc aggtccttat tcatcggcac gggttcttcg
     6721 tccatccata aatcgatatc aataccaatg tccatttcat cgcaaatcgc ccgcatgtcg
     6781 ttttctaact gttgggtgaa atcacgcagc acggcggcgt cggtatgacg acaatcaatg
     6841 gtgaacgtgg ttttacccgg caccacattc accgtattcg ggcgcggctc caccttacca
     6901 aaggtcagca ccagcgggtc gcccatcttt ttcgcttttt cgatcgactg atggcaaatg
     6961 cgactgaaag cgtaaaccgt atcacgacga taacccatcg gcgtggtgcc tgcatggttt
     7021 gattcgccgt tcagcgtcac cgtatagcga cgttgcccga caattgcatt caccacgcca
     7081 attgattgcc cattactttc cagcacacag ccctgttcga tatgcagttc gacaaaggct
     7141 ttaatatcct gacgcggagt tagcggggcg tccggcagag taaatccgca agccttcatc
     7201 gcatcgacaa aactatttcc tttggcatca cagatattcc gcacgtcgtc aggattcgcc
     7261 agtccaaaga tatttttact gccccagaaa acatacggga agcggctgcc ttcttcttcc
     7321 gccatcgcca ccacttcaac cgtacgcagc ggtgcgccgt attgcgtttt cagccagtca
     7381 attgccagcc acgccgccag cgcgccgaat tgcccgtcga ggttaccgcc gttaaccacg
     7441 gtatcgatat gcgaaccgct cagaaccact tcctgtggat attcggtgcc actcaggcga
     7501 ccgtataaat tccccacttc atcgaaacgt gtttccagcc cacttgcgcc cattcttttt
     7561 ttaaattgct gctgggtttc cagccattcc ggcgaataaa gtaaacgggt catcccaccc
     7621 gttgggtcag cgccaaatga ggaaagccag ggcagcgctt cttctatggc ttgacgaaaa
     7681 tgtgtaatca taagaaagtc ctgtctcaat aattattgcg caaagggatt tttcgtttcg
     7741 tatgacgtgt tataaagcgc gtcggaaatt aaatactggt aaatatcatc aacaatttcg
     7801 atgccttcga cggcggcttt gcgttgttta atatcctgat cctgtccggg ataataaacc
     7861 tgatttaaac tgggcgcggg ggtaatggca tttaattcgc gcatggtctg gctaagatgt
     7921 tgacggaata attcgctgga ggagaaaaag ttcgggttaa taactacatg taattgcccc
     7981 aaattacgcc cggcgtgtaa atcgtcatac atcgaactaa cctgtcgccc gaacggtaag
     8041 ccgagtaaga cgcctgagag gacgtcaatc atcatcatca ggccataccc tttcggccca
     8101 gcggcgggga gcagagcatg taccgcgaac ggatcggttg ttggtgcacc gtttttatcg
     8161 accgcccagg tatccgggat agacatatta cgcgagcggg cgtcgagcac ttttccccat
     8221 gcctgtacgg tagtcgccat atcaaaggta aggatctcgt cgccttctcc cggcgcggca
     8281 aaggccagtg ggttagtacc gtagtaaatt tccgcgccgc caaacggcac caccattgga
     8341 tcggactggc acatcgaaat gccaattaat ccggcgcggg ctgcctgctg cacaaaataa
     8401 gagattgcgc cgctgtgacc catccggctg ataccgacca ccgcaacgcc attttgctgg
     8461 gcggttttga tggcatgttc catacccatt ttcgccgcga cctgtccggc ggcattgtcg
     8521 gcatgtaaaa ttgccgagca cggcccggtt tcctcaagac gaaactccgg ttcgcggttg
     8581 gtgccgcctt ttgaaatgcg ttccgcgtag tattccacgc gcaccgcgcc atgagagtgg
     8641 atccctctgg catcggcgta caccaatact tcagccacgg ttgcagcgtg ctcacgtttt
     8701 aacccagcct ggcagagttt attctcaatt agctggtgga gtgtttcccg actgattttc
     8761 atctgtcttc ctttttaacg acggtgtgaa gcatgactgc aattaacata cagggaaaat
     8821 atctggatta tgtgatccag acaggcaaaa aaatatagtt agaatttatt tgataatctt
     8881 cgcgctttta acctggtttt taaaacaacc atgcttatta aaaaataact cgtaatagtc
     8941 tggcggttat ttgaattctt ttgttaataa tccctgtgtg atattcatca ccttatttac
     9001 tcgttgtcat cgataccgta atcgccacat taacactgct cgtgcaattg ccatgggtgc
     9061 aatttttaag gagttgttat gatccacgcc tttattaaaa aagggtgttt tcaggattcg
     9121 gtcagtttaa tgattatttc acgaaaactc agcgaatcag aaaatgttga tgatgtttcc
     9181 gtaatgatgg gtacgcccgc caataaagcg ttattagata ccacaggttt ctggcatgac
     9241 gattttaata acgccacgcc gaacgatatt tgcgtggcaa ttcgtagcga agcggcggat
     9301 gcggggatcg cgcaggcgat tatgcagcag cttgaagagg cgctaaaaca actggcgcag
     9361 gggtcaggca gcagccaggc gttgacgcag gtgcgtcgct gggacagtgc ctgtcagaaa
     9421 ttacccgatg ccaatctggc gctgatttca gtggctggcg agtatgcggc ggagctggcg
     9481 aatcaggcgc tggatcgcaa cctcaacgtg atgatgttct ccgataacgt cacgctggaa
     9541 gatgaaatcc aacttaaaac ccgcgcgcgg gaaaaaggct tgctggtgat ggggccggac
     9601 tgcggtacgt cgatgattgc cggcacgccg ctggcttttg ctaacgtgat gccggaaggc
     9661 aacattggtg tcattggcgc ttccggtacc gggattcagg agctgtgctc gcagattgcg
     9721 ctggcagggg agggaattac tcacgcgatt ggccttggcg gccgcgacct cagccgtgaa
     9781 gtgggcggca tcagtgcgct gacggcgctg gaaatgctca gtgcagacga gaaaagcgaa
     9841 gtgctggcat ttgtttcaaa accacctgcc gaagcggtgc gtctgaaaat tgttaatgcc
     9901 atgaaagcaa ccggcaaacc gacggtggcg ctgtttttag gttatacccc ggcggtggcc
     9961 cgcgacgaga atgtctggtt tgcctcctcg ctggatgagg ccgcacgcct ggcttgcctg
    10021 ctttcacgcg ttacggcgcg acgtaacgcg ataacgcctg ccagcagcgg atttatttgc
    10081 ggtttgtata ccggcggtac gctggctgcc gaagcggcgg gattacttgc cggacacctt
    10141 ggcgtggaag ccgatgatgc ccatcatcac ggcatgatgc tcgacgccga tggtcaccag
    10201 attattgacc tcggcgatga tttctacacc gtcgggcgtc cccatccgat gatcgaccca
    10261 accttacgca accagttaat tgccgatctc ggcgctaaac cgtcagtgcg cgtattgcta
    10321 cttgatgtcg tgattggctt cggcgcgacc gccgatcctg ccgcctcgct ggtgagcgcc
    10381 tggcaaaaag cctgtgccgc gcgttcagac agccaaccgc tgtacgccat tgccacggtg
    10441 acaggtaccg aacgtgaccc gcaatgccgc tcgcagcaaa tcgccacgct ggaagatgcg
    10501 gggattgcgg tcgtgagttc gctaccggaa gccaccttgc tggcggcagc gttaattcat
    10561 ccgctctcgc ctgccgcaca gcaacacaca ccgtcattac tggaaaacgt cgccgtgatt
    10621 aacatcggat tacgcagttt tgcgctggag ctacaaagcg ccagcaaacc ggttgtgcat
    10681 taccaatggt cgccagtcgc cggcggcaat aaaaaactgg ctcgtttatt agaacgtttg
    10741 caataagggg ttcccatgtt tacatcagtg gcgcaagcca atgctgcggt tatcgaacaa
    10801 attcgtcgcg ctcgtccaca ctggctggat gtgcaaccgg cttcttcact tatcagcgaa
    10861 ctacacccgg gcaaaacact gcttcacgcc gggccgccaa tgcgctggca ggagatgacc
    10921 ggacccatga aaggggcgtg cgtgggcgca tgtctgttcg aaggttgggc gaaagatgaa
    10981 gcgcaggcgc tggcaatact ggagcagggg gaagtgaact tcattccttg tcaccatgtg
    11041 aatgccgtcg ggccaatggg cggtattact tctgccagta tgccgatgct ggtggtcgag
    11101 aacgtgaccg acggcaaccg ggcgtactgc aacctcaacg aaggtatcgg caaagtgatg
    11161 cgttttggcg cttacggcga agatgtcctg actcgccatc gctggatgcg cgatgtgtta
    11221 atgccagtat taagcgcggc gctggggcgc atggagcgcg gtatcgatct tactgcgatg
    11281 atggcgcagg gcattaccat gggcgatgag ttccatcaac gcaatattgc ttcctcagca
    11341 ctgttaatgc gtgcgctggc cccacaaatt gctcgcctcg atcatgataa acagcacatc
    11401 gcggaagtga tggatttcct cagcgtgacc gatcagttct tcctcaacct cgcgatggct
    11461 tactgcaagg cggtgatgga tgctggcgcg atgatccgcg caggcagcat cgtcacggca
    11521 atgacccgca acggcaatat gttcgggatt cgggtaagcg ggctgggtga acgctggttt
    11581 actgcgcctg taaacactcc gcaaggtctg tttttcaccg gcttctcgca ggagcaggcg
    11641 aacccggata tgggcgatag cgcgattacc gaaacctttg gtatcggagg tgcggcaatg
    11701 atcgcagcgc ctggcgtaac gcgctttgtc ggtgcgggtg gcatggaagc ggccagggcg
    11761 gtatctgaag agatggcgga aatttacctt gaacgcaata tgcagttgca gatcccaagc
    11821 tgggattttc agggcgcgtg cctgggactg gacattcgtc gcgtggtaga aaccggcatt
    11881 acgccactca tcaataccgg tatcgcccat aaagaggcgg ggatcgggca gattggcgca
    11941 ggcaccgtgc gggcaccgct ggcgtgcttt gaacaggcgc tggaagcact ggctgaaagc
    12001 atgggtattg gttgaggaac gcgcaatgac gatcatccat cctctgcttg ccagtagtag
    12061 cgcaccgaat tatcgccagt cctggcggtt agcgggagtg tggcggcggg cgattaacct
    12121 gatgacggaa agcggcgaac tgttaacgct gcatcgtcag ggtagtggtt tcggccccgg
    12181 aggatgggtg cttcgccgtg cgcaattcga tgcgttatgc ggtggattat gcggcaatga
    12241 acgaccacag gttgtggctc aagggattcg cctcgggcgt ttcacggtta aacagccaca
    12301 gcgttattgt ttgctgcgta ttacgccgcc tgcgcatcct caaccgcttg tagctgcatg
    12361 gatgcaacgc gcggaggaaa ccgggctttt cgggccactg gtgttggcgg caagcgatcc
    12421 gctgcctgct gaattacgcc agtttcgtca ctgttttcag gccgcgctca atggcgttaa
    12481 gaccgactgg cggcactggc tgggtaaagg ccccggatta acgccgagtc atgatgacac
    12541 gctgagcgga atgctgctgg cggcctggta ttatggcgct ttagatgcgc gctccggtcg
    12601 gccgtttttt gcctgttccg acaatctgca actcgttacc acagcggtga gcgtcagtta
    12661 tttacgttat gccgcgcaag gatatttcgc ctcgccactc ctgcactttg ttcatgctct
    12721 gagttgcccg aaacgtaccg ctgttgcgat tgattcgctg ctggcgctgg ggcatacgtc
    12781 aggggcagat acactgctgg ggttctggct tggtcaacaa ttattacaag gaaaaccatg
    12841 aaaacactgg ttgtagctct tgggggcaac gccttactcc agcgcggtga ggcgctgacg
    12901 gcggaaaatc aatatcgcaa tatcgccagt gctgtccccg cgctggcgcg cctggcccgt
    12961 tcttatcgac tggcgattgt tcacggcaac ggaccgcagg tggggctgct ggcgttacag
    13021 aatctggcgt ggaaagaggt agaaccgtat ccgctggatg tgctggtggc agaaagtcag
    13081 gggatgattg gctatatgct ggcgcagagc ttgagcgcac agccgcagat gccgcctgtt
    13141 acgacggtgc tgacgcgcat tgaggtttcg cctgatgatc cggcgttttt gcagccagag
    13201 aaatttattg gtccggttta tcagccagaa gaacaagagg cactggaagc ggcttacggc
    13261 tggcagatga aacgtgatgg taaatatttg cgccgggtgg tggcgtctcc gcaaccgcgt
    13321 aaaattctcg acagcgaagc catcgagttg ttgctcaaag aggggcatgt ggtgatttgc
    13381 agtggcggcg gcggtgtgcc tgtgacggaa gacggggcag ggagtgaagc agtgattgat
    13441 aaagatctcg ccgctgcgtt gctcgccgag cagattaacg ccgatgggct ggtgatcctc
    13501 accgatgctg atgcggtata tgaaaactgg ggaacgccgc agcaacgtgc cattcgccat
    13561 gccacaccgg atgagttagc gccatttgcc aaagccgatg gttcgatggg gccgaaggta
    13621 acggcggtga gtggttatgt cagaagccgt ggtaaacccg cgtggattgg ggcgttatcg
    13681 cgaattgaag agacgctggc gggcgaagcg gggacctgta tttcgctgta gtgatgagag
    13741 catccagcaa tcggtgcacc atgcctgatg cgacgcttga cgcgtcttat caggcctaca
    13801 atgggtatcg gagcggtaag ccggataagg cgtttacgcc gcatccggca atcaaatgag
    13861 cacctgctta actgaactta ctctgcgccc acatcacgcc gctggcatac tctggcggca
    13921 gcagcgggat taaggcttcc agcgtcgcgg tcagacgcga tgtgtcgctg tcggttaaat
    13981 tcagatgccc cactttacgc cccggacgga cttctttgtc gtaccagtgc agatgcacca
    14041 gcggcaattt cagccagtca taattcacat cgctaccaat caggttgatc atcaccgacg
    14101 gactattcac tatcggttgc ggtaacggca gagcggtaat cgcccgcaga tgtagctcaa
    14161 actggctgat gctggcaccg ttttgtgtcc agtgaccgct gttatgcaca cgcggtgcca
    14221 gttcgttgat cagcagacct tgcggggtga caaaacactc catcgccatc acgcccacat
    14281 agcccagctc ctgcataatc gccgacagca tctcttcggc ttgcgcttgc tgctgcgcat
    14341 tggcctgcgg aaaagcgacg ctggtgcgca aaataccgtc ctgatgcagg ttatgcgtca
    14401 gcggataaaa cacggtgctg ccatcaaagc cgcgcgcgcc aaccagcgat acttcaccag
    14461 agaagttaat gccttgctcg acaatacatt cgccgtaaca ctcttccggt aactgttcgg
    14521 tttcatcggc gcgtaaacgc cattgaccgc gtccgtcata gccaccagtg cgacgcttaa
    14581 caatcgccag ctcacctaaa cgatcaaaca ccgcaggcca ctcgctgcga tcggcaagta
    14641 actgccacgg tgcggtcggc aggtggagct tatcgaaaag ctgcttctga gtcagacggt
    14701 cggcaataat cgggaacaca tcgcggttca caaaggccgg atggcgcgcc agctcgcggg
    14761 ttaatgcggt ttccggccaa cgttcgatct cagcggtaat cacgctttgt tgaaaaggca
    14821 ccgccgccgg ttcagcgtcc agcccgaccg gccagacagc aatgcctaac ggttcgcctg
    14881 cctgacgcag catacggcct aactgcccgt taccgaggac gcaaacctgt ttcatgccgc
    14941 acctcgcggg tccgggtttt ccagcacttc atcagtttgt gctttgcgcc agtcattcag
    15001 acgctggtgc agttctttat catgagtcgc aagaatttgt gctgccagta atgccgcgtt
    15061 tgccgcgcca gctttaccaa tcgccagcgt acccaccgga atgccgcgtg gcatttgtac
    15121 gatggagtag aggctatcga caccgctcag tgcggcgctc tgtactggca cgcccagcac
    15181 tggcaccagc gttttggcgg caatcatacc tggcaggtgc gctgcgccgc ctgcgcccgc
    15241 aataatcacc tgataaccgt tctcttcggc gctttcggcg aagctgaaca gtttatcggg
    15301 ggtgcggtga gcagaaacca cttcaacgtg gtgcgggaca ttcaggattt cgaagatttc
    15361 ggcggcgaac tgcatggtag cccagtcgct tttggacccc atcacgatgg cgacacgcgc
    15421 cggattattg cgggaagaca tgcgtcttaa aactcctgtg gtgcacaact ctcggcttta
    15481 gagggcacag agaatagcac ggaaagagag caaggaaaac ggttgcgtgg ctatgaaatc
    15541 agcaaagttg cgggtttttt aaaacggaaa atgaatcagc tcaacgtcat ccgccgtgac
    15601 tttcaccatc gaaccttccg tatgccaggc acccagtacc acgcgaaaag caggttgctg
    15661 attggcgata agttcatgta ccgccgggcg atgggtatgc ccgtggatca gccattgcac
    15721 ctgatgtttt tccatcgcac tgaccaccgc gttttggtta acgtccatga tcgccagcga
    15781 tttactgctg ttggcttctt tactgttcgc gcgcattcgc gcggcaatgc gtttgcgcac
    15841 aaacaacggc agagcgagga atagcgtttg cagccagggt ttgtggactt tggtgcgaaa
    15901 agcctgataa cccgcgtcat cggtgcacag cgtgtcgcca tgcataatca gcacccgacg
    15961 accataaagt tcgagcacct tttcttccgg caataacgtc atgccgcttt cacgggcaaa
    16021 gcgtttgccg agcagaaaat cacggttgcc atgaatgaaa taacagggaa cgccggaatc
    16081 ggacaccgct ttgatcgctg ccgccatctg gcgatggagt gggttgggat cgtcgtcgcc
    16141 aatccacgct tcaaacagat cgccaagaat atacagcgcg tcggccttgc gagcttcccc
    16201 cgctaaaaaa cgcagaaaac cggcggtgat cgccggttct tccacgcaga gatgaagatc
    16261 tgcaataaag agtgtcgcca cgattactcg ctaacggtca cgctttcaat gataacgtct
    16321 tcttttggca cgtcctggtg cataccgcta cgaccggttg caacaccttt gattttgtct
    16381 accacgtcca tgccttcaac cacttcagca aacacgcagt agccccaacc ttgcaggctt
    16441 tcgccagaga agttcaggaa gtcgttatca accacgttga tgaagaactg tgcagttgca
    16501 gagtgcggag cctgagtacg ggccattgcc agcgtaccac gggtattttt cagaccgttg
    16561 ttggcttcgt ttttgatcgg ttctttggtg gctttttgtt tcatgcccgg ttcaaaacca
    16621 ccgccctgaa tcataaagcc gttgataaca cggtggaaaa tggtgttgtt gtaaaaacct
    16681 tcgcggcagt agtccaggaa gtttttaact gtttcaggtg ctttatcgtc aaaagttttg
    16741 atgacaatat cgccgtgatt ggtgtggaaa gtaaccattt ttgcatcctg ttccgtttga
    16801 ttggtgcttc aacccagttc gggtcatata tagggtggtg ttatagcata accgcacgat
    16861 cggatcatca cgcaatgtat gctgattcgc gcgggaaata tgggtattat acgcaactca
    16921 attacccaca catgtctaaa cggaatcttc gatgctaaaa atcttcaata ctctgacacg
    16981 ccaaaaagag gaatttaagc ctattcacgc cggggaagtc ggcatgtacg tgtgtggaat
    17041 caccgtttac gatctctgtc atatcggtca cgggcgtacc tttgttgctt ttgacgtggt
    17101 tgcgcgctat ctgcgtttcc tcggctataa gctgaagtat gtgcgcaaca ttaccgatat
    17161 cgacgacaaa atcatcaaac gcgccaatga aaatggcgaa agctttgtgg cgctggtgga
    17221 tcgcatgatc gccgaaatgc acaaagattt tgatgcgttg aacattctgc gcccggatat
    17281 ggagccgcgc gcgacgcacc atatcgcaga aattattgaa ctcactgaac aactgatcgc
    17341 caaaggtcac gcttatgtgg cggacaacgg cgacgtgatg ttcgacgtcc cgaccgatcc
    17401 aacttatggc gtgctgtcgc gtcaggatct cgaccagctg caggcaggcg cgcgcgttga
    17461 cgtggttgat gacaaacgca acccgatgga cttcgttctg tggaagatgt cgaaagaggg
    17521 cgaaccgagc tggccgtctc cgtggggcgc gggccgtcca ggctggcaca ttgaatgttc
    17581 ggcaatgaac tgcaagcagc tgggtaacca ctttgatatc cacggcggcg gttcagacct
    17641 gatgttcccg caccacgaga acgaaatcgc tcagtccacc tgtgcgcatg atggccagta
    17701 tgtgaactac tggatgcact ccgggatggt gatggttgac cgcgagaaga tgtccaaatc
    17761 gctgggtaac ttcttcaccg tgcgtgatgt gctgaaatac tacgacgcgg aaaccgtgcg
    17821 ttacttcctg atgtcgggcc actatcgcag ccaactgaac tacagcgaag agaacctgaa
    17881 gcaggcgcgt gcggcgctgg agcgtctcta cactgcgctg cgcggtacgg ataaatctgt
    17941 tgcccctgcc ggtggtgaag cgtttgaagc gcgctttatt gaagcgatgg acgacgattt
    18001 taacaccccg gaagcctatt ccgtgctgtt tgatatggcg cgtgaagtga accgtctgaa
    18061 agcagaagat atggcagcgg cgaatgcaat ggcatctcac ctgcgtaaac tttccgctgt
    18121 attgggcctg ctggagcaag aaccggaagc gttcctgcaa agcggcgcgc aggcagacga
    18181 cagcgaagtg gctgagattg aagcgttaat tcaacagcgt ctggatgccc gtaaagcgaa
    18241 agactgggcg gcggcagatg cggcgcgtga ccgtcttaac gagatgggga tcgtgctgga
    18301 agatggcccg caaggaacca cctggcgtcg taagtaattg cgctattgcc ggatgcgagt
    18361 tttcgcatcc ggttatcgtc tgcgccacca caacattccc atcagtagca tccccggcaa
    18421 ccacacccac atcaattcag aaataatcac ctgatgcccg tacgctgtgg tgtaacgaga
    18481 caatgcaaac ggcgcgactt taatcacctg ccagggagcg aaaaagcgtt catctgacca
    18541 cggccacagc cagccaacgc ccttaccgcc agtggttacc gaatccagta agctgtgcga
    18601 tagcaacgag acggttaaaa acagccagca gcgaatcagc ccagccctga accatcggcg
    18661 tccaatcagc acacataaca gcgggacaac aaacgcaaac accagcgaat gggtaaaccc
    18721 gcgatgacca aaaacattgc cgtaagcaat gccaaattta aacgacaaga cgtcggcatc
    18781 gggcagcatc gccaggatga ttccggcaaa taacaggcgc ggagggatga ctttcgaacc
    18841 caaccctaaa ccaatgcata gaggaacggc ggcgtgcgta ataacggttg gcatgatggt
    18901 cgcttcggca aaatgtcgat gctatcagca tgaatgaatg gggcgtagag tgcaaaagtc
    18961 tgaaaagaga accggcctgt tgatacaggc cgggaaaggg atcaggcaac aacctgtacg
    19021 ctgtgacctg caaaactcac tgtctgaccg gcgacgattt tgcagcgttt gcgcgtttca
    19081 accgcgccat caactttcac ctggccttcg gcaatcgcga ttttcgcctg cgcgccgctt
    19141 tcgctccagc cttccagctt cagcaagtcg cacagctcaa cgtgcggatg tttacctaaa
    19201 gaaaatgtcg ccatgttact caccctgtgg atcatgatat tcaacgcacg cctgtagcgt
    19261 gttttcaatc agcgtggcaa ccgtcatcgg gccaacgccg cccggaacag gcgtaatgta
    19321 tgaggcgcgt ttagccgcgt cttcaaacac cacgtcgccc acaactttgc cattttccag
    19381 acggttgatg ccgacatcaa tcacaattgc gccttctttg atccagtcac cgggaataaa
    19441 acctggctta ccgacggcaa cgatcagtag atcggcattt tctacgtggt gacgcagatt
    19501 tttagtgaaa cggtgagtca cggtggtggt gcaacctgcc agtagcagtt ccatgctcat
    19561 cgggcggcca acgatattcg atgcgccaat caccacggcg ttgaggccga aggtatcaat
    19621 gttgtaacgc tcaagcagcg tgacaatacc gcgcggggtg cagggacgca gacgcggcgc
    19681 gcgctggcac aggcgaccga cgttataagg atggaaaccg tctacgtctt tgtccgggtg
    19741 tatacgttcc agcactttga cgttatcaat acccgccggt aacggcagtt gaaccagaat
    19801 gccatcgatg gtgttatcgg cattcagcgc atcgataagc tccagcagct ccgcttcgct
    19861 ggtggtttcc gggaggtcgt aagagcggga gacgaacccg acttcttcac aagccttgcg
    19921 tttgcttgcg acataaattt gcgatgcagg gttactaccc accagcacaa cggccagtcc
    19981 tggtgcccgc agtccggctg caatacgcgc ctgaactttt tgagcaactt cagagcgcac
    20041 ctgctgcgca atcgttttac cgtcaataat ctttgctgcc atcagagaga ggattccatc
    20101 tgttacgtag gtcgaagggg atgcgcctat tttgtcagaa gcggggcgcg ctgtcaggtt
    20161 tcgtttcaga tttatcgcgt gaagcgacct cttgcgaagg tgaggcgcac tgtcgctgag
    20221 attgaaagct tcatttttcg tccatgatgg cgttgtaaat ctggaactga tttatttcct
    20281 tgtctaagga ttaagataat ttaagaaata cctgacaata taaaaagaat tttcagcctg
    20341 gtaatttacc gcttcaggtc tatatttgt
//
LOCUS       DAPGOF010000084        20315 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.084, whole genome shotgun sequence.
ACCESSION   DAPGOF010000084 DAPGOF010000000
VERSION     DAPGOF010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20315
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.084"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(241..987)
                     /gene="cutC"
                     /locus_tag="RHV13_002661"
     CDS             complement(241..987)
                     /gene="cutC"
                     /locus_tag="RHV13_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDV1242183.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRERVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMCYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(1001..1567)
                     /locus_tag="RHV13_002662"
     CDS             complement(1001..1567)
                     /locus_tag="RHV13_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDV1242184.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1783..3516
                     /gene="argS"
                     /locus_tag="RHV13_002663"
     CDS             1783..3516
                     /gene="argS"
                     /locus_tag="RHV13_002663"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDV1242185.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDDEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEIDEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            3693..4181
                     /locus_tag="RHV13_002664"
     CDS             3693..4181
                     /locus_tag="RHV13_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HDV1242186.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVSKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4303..4695)
                     /gene="flhe"
                     /locus_tag="RHV13_002665"
     CDS             complement(4303..4695)
                     /gene="flhe"
                     /locus_tag="RHV13_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HDV1242187.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(4695..6773)
                     /gene="flhA"
                     /locus_tag="RHV13_002666"
     CDS             complement(4695..6773)
                     /gene="flhA"
                     /locus_tag="RHV13_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HDV1242188.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATIDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6766..7914)
                     /gene="flhB"
                     /locus_tag="RHV13_002667"
     CDS             complement(6766..7914)
                     /gene="flhB"
                     /locus_tag="RHV13_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HDV1242189.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(8116..8760)
                     /gene="cheZ"
                     /locus_tag="RHV13_002668"
     CDS             complement(8116..8760)
                     /gene="cheZ"
                     /locus_tag="RHV13_002668"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HDV1242190.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8771..9160)
                     /gene="cheY"
                     /locus_tag="RHV13_002669"
     CDS             complement(8771..9160)
                     /gene="cheY"
                     /locus_tag="RHV13_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HDV1242191.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(9175..10224)
                     /gene="cheB"
                     /locus_tag="RHV13_002670"
     CDS             complement(9175..10224)
                     /gene="cheB"
                     /locus_tag="RHV13_002670"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HDV1242192.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELSRSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(10227..11087)
                     /gene="cheR"
                     /locus_tag="RHV13_002671"
     CDS             complement(10227..11087)
                     /gene="cheR"
                     /locus_tag="RHV13_002671"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HDV1242193.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(11106..12707)
                     /gene="tap"
                     /locus_tag="RHV13_002672"
     CDS             complement(11106..12707)
                     /gene="tap"
                     /locus_tag="RHV13_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HDV1242194.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAK
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(12753..14414)
                     /gene="tar"
                     /locus_tag="RHV13_002673"
     CDS             complement(12753..14414)
                     /gene="tar"
                     /locus_tag="RHV13_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HDV1242195.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(14559..15062)
                     /gene="cheW"
                     /locus_tag="RHV13_002674"
     CDS             complement(14559..15062)
                     /gene="cheW"
                     /locus_tag="RHV13_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HDV1242196.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(15083..17047)
                     /gene="cheA"
                     /locus_tag="RHV13_002675"
     CDS             complement(15083..17047)
                     /gene="cheA"
                     /locus_tag="RHV13_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HDV1242197.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(17052..17978)
                     /gene="motB"
                     /locus_tag="RHV13_002676"
     CDS             complement(17052..17978)
                     /gene="motB"
                     /locus_tag="RHV13_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HDV1242198.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(17975..18862)
                     /gene="motA"
                     /locus_tag="RHV13_002677"
     CDS             complement(17975..18862)
                     /gene="motA"
                     /locus_tag="RHV13_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HDV1242199.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(18989..19567)
                     /gene="flhC"
                     /locus_tag="RHV13_002678"
     CDS             complement(18989..19567)
                     /gene="flhC"
                     /locus_tag="RHV13_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HDV1242200.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(19570..19920)
                     /gene="flhD"
                     /locus_tag="RHV13_002679"
     CDS             complement(19570..19920)
                     /gene="flhD"
                     /locus_tag="RHV13_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HDV1242201.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         4883 a         5574 c         4935 g         4923 t
ORIGIN      
        1 gaataaccca gttgctattt agaatatata tttatcagtt ttagtaattt aaatcccata
       61 attaatgtga atatataaaa tatttttagc acaacgctat gttcactgtc acccgctcaa
      121 cagatcacgg taatcgttcg ttttttatac tactcaggga taaaaaaagg cccctgttga
      181 aattgcaggg gcctggtacg agcaagcatc atattgggcg acatgatgca acggtaaaaa
      241 tcatttggcc tgatggcgtt caatgattcc tttcatttca gcaaccgccg ccccgtctac
      301 gacataacgc gaatactcgt ccgcgtgtgc atctgatgac atggacaatc cttgattacg
      361 ataacacatc ggtgaggctt gccacgctcc cgcggagcta tggacttcca gcactccggc
      421 atcgaggaag tggtgcaggt tttctgcacg gactcctgct ccggccataa tgattggagc
      481 atcacgatgg gcaataagtt ccataatttt tgataaacct tgcagtgcgt ctgatttttg
      541 ccctgatgtc agtacccggg caatgcccaa ttccgcaaga ttattaagtg tatttaaagg
      601 gttagcgcac atatcgaagg cgcgatgaaa agtcactgcc agcggaccgg cagcagccat
      661 tattttttcc attcgtggca tatcgacatt cccgtcaaca tcgagcacgc ccgtcaccag
      721 tccaggaaaa cctaattcgc ggaccgtgcg cacatcctca agaatggcgg caaactcacc
      781 gtcgctgtag caaaaatcac caccgcgtgg gcgaattatc ggatgcacag ggatcgtcac
      841 ccgctcgcgc acggatttca gtacacccaa cgacggcgtt aagcccccct cttttggggc
      901 tgcgcataat tcaactctgt ctgcgccgtt ttgctgcgcc gttagtgcac attccatgct
      961 gtaacagcaa atttccagta atgccatttt tactccttaa ttacgccgac tgctcgctgg
     1021 caacgatctc ttcaatggac cacggatgaa acttaatagt tgttttgcca tcggtgaccg
     1081 ccagcgtcgg attcggtaat cgctcatgct caccttttgg cgaactggtt ttcactgaga
     1141 tccccggtag gctgagtcct tcatcagaaa gcaatgccag cgcacgggca ttcagcgttt
     1201 ctggatcacc cggcagaacg atttcaatat gttcccaacc ttcgtgcggg taacgttttt
     1261 ccccgggcca cggtagctcc acaatggaaa actgccagtg agcaacctgt accggttcat
     1321 gcaatttaaa cagacaaatc ggtctgccat tgatcatatt ttctgacaaa agctcgccac
     1381 actgttcaaa cccgcgacgc cagcgttcag cagtggcgtt ttgatggcaa cgcaaagaaa
     1441 tgtgatcggc agtcagcgga gtgatattta accccagacg gcgggaaagt tcatctaatg
     1501 cgtgggtaaa tcgcggtaaa tccgatgcaa tatcctgcag ctcgtcgata gattgccagt
     1561 tcgccataat cactcttcgt ctttcagtaa aagcgttaat ttaccctgtt gccctgtgcc
     1621 aaccaaccgc tgatttcacg ccgcttctga tgcaatagtg aaaacggcaa tacgccacgc
     1681 gcacgttgct gacgaaaaca gccatttgca gtatactccc gccctaattt ctttaactgg
     1741 tgcgggcaat ttttgctcgc ttcatcaatg taaggtattc cggtgaatat tcaggctctt
     1801 ctctcagaaa aagtccgtca ggccatgatt gcggcaggcg cgcctgcgga ttgcgaaccg
     1861 caggttcgtc agtcagcaaa agttcagttc ggcgactatc aggctaacgg catgatggca
     1921 gttgctaaaa aactgggtat ggcaccgcga caattagcag agcaggtgct gactcatctg
     1981 gatcttaacg gtatcgccag caaagttgag atcgccggtc caggctttat caacattttc
     2041 cttgatccgg cattcctggc tgaacatgtt cagcaggcgc tggcgtccga tcgtctcggt
     2101 gttgctacgc cagaaaaaca gaccattgtg gttgactact ctgcgccaaa cgtggcgaaa
     2161 gagatgcacg tcggtcacct gcgctctacc attattggtg acgcagcagt gcgtactctg
     2221 gagttcctcg gtcacaaagt gattcgcgca aaccacgtcg gcgactgggg cactcagttc
     2281 ggtatgctga ttgcatggct ggaaaagcag cagcaggaaa acgccggtga aatggagctg
     2341 gctgaccttg aaggtttcta ccgcgatgcg aaaaagcatt acgacgaaga tgatgagttt
     2401 gccgagcgtg cacgtaacta cgtggtaaaa ctgcaaagcg gtgacgaata tttccgcgag
     2461 atgtggcgca aactggtcga catcaccatg acgcagaacc agatcactta cgatcgtctc
     2521 aacgtgacgc tgacccgtga tgacgtgatg ggcgaaagcc tctacaaccc aatgctgccg
     2581 ggtatcgtgg cggacctgaa agccaaaggt ctggcagtag aaagtgaagg cgcgaccgtc
     2641 gtattccttg atgagttcaa aaacaaggaa ggcgaaccga tgggcgtgat cattcagaag
     2701 aaagatggcg gctatctcta caccaccact gatattgcct gtgcgaaata tcgttatgaa
     2761 acactgcatg ccgatcgcgt gctgtattat atcgactccc gtcagcatca acacctgatg
     2821 caggcatggg cgatcgtccg taaagcaggc tatgtaccgg aatccgtacc gctggaacac
     2881 cacatgttcg gcatgatgct gggtaaagac ggcaaaccgt tcaaaacccg cgcgggtggt
     2941 acagtgaaac tggccgatct gctggatgaa gccctggaac gtgcacgccg tctggtggca
     3001 gaaaagaacc cggatatgcc agccgacgag ctggaaaaac tggctaacgc ggttggtatt
     3061 ggtgcggtga aatatgcgga tctctccaaa aaccgcacca cggactacat cttcgactgg
     3121 gacaacatgc tggcgtttga gggtaatacc gcgccataca tgcagtatgc atacacgcgt
     3181 gtattgtccg tgttccgtaa agcagaaatt gacgaagagc aactggctgc agctccggtt
     3241 atcatccgtg aagatcgtga agcgcaactg gcagctcgcc tgctgcagtt tgaagaaacc
     3301 ctcaccgtgg ttgcccgtga aggcacgccg catgtaatgt gtgcttacct gtacgatctg
     3361 gccggtctgt tctctggctt ctacgagcac tgcccgatcc tcagcgcaga aaacgaagaa
     3421 gtgcgtaaca gccgtctgaa actggcacaa ctgacagcga agacgctgaa gctgggtctg
     3481 gatacgctgg gtattgagac tgtagagcgt atgtaatcga tttttcgtga gagtgaagcc
     3541 tgatcaggtt tagccgatca ggctttttta ttgccatcta aatgtattct gaaaacggac
     3601 atgccatcgt tttttcactg ttgagtaaga ggccagaagc gtaatatcca ggcccaggaa
     3661 aacgataacg gttcaattta aggaataccg cagtgtttaa atttcttgta ttaacattag
     3721 gcattatctc ttgccaggct tacgcagaag atacggttat agtaaacgac catgacattt
     3781 cagccatcaa agattgttgg caaaaaaatt cagatgatga tactgacatt aatgtgagca
     3841 aatcatgcct gcgacaagaa tacaatctcg tcgatgcgca attaaataaa gcttatggtg
     3901 aagcttatcg ttatatagaa caagtgccac gcacaggtgc aaaaaaacct gataccgaac
     3961 aacttaactt gcttaaaaaa tcacaacgag cctggctgga ttttagggac aaagaatgtg
     4021 aattaatcct ttcaaatgag gatgttcagg atttaagtga cccttattct gaatcagaat
     4081 ggctatcatg tatgatcata cagaccaata cgcgaactcg ccagttgcag ctataccgta
     4141 actctgaaga tttttatcca agccctttga caagaggata attcacatct ttttggcatg
     4201 ttttgttgca agctattcct gataaataat tgcaacaaaa catcgagcct ttttcgctga
     4261 gttattaaac atgctcgcga gcgcgtaatt tttatttgtc cttcagcgat aattcacaat
     4321 cacttcattc cgttgtacct ttagcggtgg aattaaccgc ccaccgccag gcacttccca
     4381 gataaagcgc aacggttctg ctgccgctat accggaaaag gccacggtgg ttccgctctg
     4441 cccctctaat tcgacacaac gagattgcga acacaagcga acccgcagtc ctgcaggtgt
     4501 cgggccgata agctgataac gccacgccac cagcgtcatc aatcctgaag caggttgtcg
     4561 agtagaaaga ggcgctgacg acatcgactc accgcgatga tttagcgtaa ttccgacact
     4621 acttgcctgc cacatcccct ccccggctgc ttgcactagc agcggaaaca ataatattgc
     4681 taataaggtt ctcatcattt accgccaatt gtcgccgtca tgcggatatg tcggttatca
     4741 gatagttcca gattcgacag caccactaac tgcggcaagc tgcggcgcag aaagcgagac
     4801 aataatggtc gcagcgcgtg gttcaccaac aatactggcg gcgcacccaa catctcctga
     4861 cgggatagcg cttcctgagt ttgcgccagt aaacgatccg ccagccctgg ctccagtccg
     4921 cccccgccct gcaacgcctg tagcaacaaa cgttccagcg gtgtatcgag gccaataaca
     4981 tggacttcat ctttgccagg aaaccactgc tgggtaatcg cccgtcccaa cgccacgcgc
     5041 acgacggcgg ttaattcatg tggatcactt tggatgggtg catgttccgc cagcgtttcg
     5101 agaatagtgc gcatatcgcg aatcggcact ttttcatcga gcagattttg cagcacttta
     5161 tgcagtgtgg tgagcgtgac gacgccagga acgagatctt ccgtcagctt tggcatctcc
     5221 tgggcgacgc gatccagcag ctgttgcgcc tcctgacgac caaacagctc tgcggcatgc
     5281 tggctaatga ggtggttaag atgcgttgct accaccgtgc tagcctcaac cacggtgtac
     5341 ccctgaatct gcgcctgttc ttttagcgca ctttcaatcc agatagcatt caggccaaat
     5401 gccggatcga tggtcgcctc accaggtaac gtcccggcag cggttccagg gttaatcgcc
     5461 aaccagcgcc ccggataagc atcaccactg cctatctcca cgcctttcat caaaatgcga
     5521 tagcgggcag gttgcagatc catattgtcg cgaatgtgca ccactggcgg cagaaatccc
     5581 atctcctggg caaatttctt gcggatactg cgtatacggc ccaacaactc accatcctgc
     5641 tggaaatcga ccatcgggat cagtcgataa cccacttcca ttcccagaga atcttccagt
     5701 tgtacatcgt tccacgtcgc ttcgacaacg gtattattct ctgccatttt taccggtttg
     5761 ggttcggcag gcgctttttg ttcgcgtccg cgtatccacc aggccagccc gagcaatccg
     5821 gcagtgaaca gcaaaaatac caggttcggc attccaggca ccaggccgag taaaccgagc
     5881 acggcggcgc ttaacaacat aacgcttggg ttactgaaaa gctgattcac catctgctcg
     5941 ccaacatcct gatcggtgct gacacgcgta acgatgaccc ccgcggcggt agaaatcacc
     6001 agcgccggaa tttgtgccac cagaccgtcg ccaatggtca atagcgtata actttccgcc
     6061 gcgtgtccca tgctcatgcc atgttgcagc acgccgacca gcaacccgcc gacaacgtta
     6121 atgaccatga tgaggatccc ggcgatggca tcgccgcgaa caaacttact tgccccgtcc
     6181 attgagccgt aaaaatcggc ttcctgagtc acttcggagc ggcgtttttt cgcctcatct
     6241 tcaccaatca atccggcgtt aaggtcggcg tcaatcgcca tctgcttacc cggcatacca
     6301 tcgagaacaa agcgcgcacc cacttctgcg atacgcccgg cacctttggt aatgaccata
     6361 aagttgatga tcacgagaat gacaaacacc acgataccga tagcgaaatt gccaccaacg
     6421 aggaagtgac cgaacgcttc gaccaccttc cctgccgccg ctgcgccggt atgcccttcc
     6481 atcaaaatga tacgggttga agccacgtta agtgccagac gcaacagcgt ggtaaacaac
     6541 agaatggtcg gaaacgcagc aaactcaagc gtgcgctggg taaacatcgc caccagcaac
     6601 accatgatcg acaaggcaat attgaaggta aacaacaggt cgagtatgaa tgcgggcagt
     6661 ggcagcacca tcatcgacaa gatcaacagg atcaaaatcg gtccggcaag gatctgccat
     6721 tgtgtcgatt tcaggtttgc gggcaggcgc agcatcgcgg ccagattact catgggtcgg
     6781 tttctcgtta ataaaatcca gggcttccgg caccggaaga tgagtaggtt gtacagggcg
     6841 ctgtccacca gccagacgcc agcgtttcag ttgccagacc caggccagca cttccgccac
     6901 cgcggcgtac agttgacccg ggatttgttg accaatctcc gcatgtcgat acagcgctcg
     6961 cgccagcggc ggcgcttcaa gcgtcgggac gttattttca gcgccaattt cacgaatgcg
     7021 cagcgcgacc agccctgcac ctttagcgac cactttcggt gcgctcattt tgttttcgtc
     7081 atactgcaac gctaccgaat agtgggtcgg gttattgaca atgacatccg ctttcggcac
     7141 atcggccatc atccgacgcc gtgcggcagc tcgctgcatc tgacggatcc gtcctttaac
     7201 atgggggtcg ccttcgcttt gtttgaactc atcacgaata tcctgccgcg acattcgcag
     7261 ctttttcagg tggctgaata tttggaaaaa gacgtcaaat cccaccattg gaatgacacc
     7321 aagcaccacc agcagtgcgc atagccctac caaatccatc gcattaccca tggcggtaat
     7381 cggagactcg gccattaagc gcatcatctg cggccagtga tgccagagaa aaaaccccgt
     7441 cacgctgcca accaggatgg ttttcaaaat tgctttaagc aactccgcgc cagtctgagc
     7501 cgagaacatc cgtttaatgc ccggtagcgg gttgagtttg gaaaacttcg gctgcaagga
     7561 tttgccgcta aataccagcc ctcccagcat gaccggagaa atgagcgcca ccagcaccac
     7621 gccgctaatc agcggcagca gcgccagcat ggcttctctg atcagcagaa taatctgccc
     7681 gaggatcaga ttcgggtcat tgataatact gtgatcaaaa tgcagcccag cggagagcat
     7741 gcccgacaat cgacgggcca gcgacacacc gccaaaccag ataacactaa cgcccaccag
     7801 caaaatcagc agtgaggtca gttcacggga acgcgggatt tgcccctctt cccgcgcttt
     7861 ttctagtcgg tgaggtgtgg gggcttctgt tttgtcgtcg ctctcgtcag acacgtcgcc
     7921 aatcctggaa agagtattaa gcgtgaatga tgccagagcg caaagcgttc aatggtttga
     7981 gtaaggggca aaacaggcgg gatttagggc ttttgctgcc acacatcaag catagtgtgc
     8041 gtttgtcgga tgcggcgtca tcgccttatc agaccgcctg atatgacgtg gtcacgccac
     8101 atcaggcaat acaaatcaaa atccaagact atccaacaaa tcgtccacct gatcctgact
     8161 ggctaccaca ccggctttgc tggtatcgac ctgaggtcca ttaagcaaac tctggttttc
     8221 acgttttgga cgcgactcct gttccgggat gttttccaac agcaccatca gcaactggcg
     8281 ttcgatctcc tgaatgacat ccatcatccg cttaatgacc tgcccggtga gatcctgaaa
     8341 atcctgcgcc atcatgattt ccagcagttg cgcgttagta aagctggtat gcgcgggtac
     8401 atctgccaga aattgtcgtg tatctgttac cagttcacgg gcgtcggcaa ggtcaatcgg
     8461 atcggcaaac cagtcatccc aacgttgggt taacgctttt gctgatttct ccatttgatc
     8521 ctgatgcggt tgtgacgcct caacactgtt cagcgcccgc tccgcagcct gggcggtcat
     8581 ctgcacaaca tagtacaaac gatcgcgcgc atcggggatg gcttccgccg cttcggcaat
     8641 ggcctgatcc agccccagtt cccgcaaact gtcgcgcagc atacgcgtca ggctgccgat
     8701 gcgcgcaatg atatcgccag ctgaatgctc gtcagcaggt ttgattgatg gttgcatcat
     8761 agtcgcatcc tcacatgccc agtttctcaa agattttgtt gagtttttcc tccagcgtcg
     8821 cggcggtaaa tggcttcacc acatagccac tggcccccgc ttgcgccgca gcaatgatgt
     8881 tctctttctt cgcttctgca gtcaccatta acactggcaa tgccgacatc gcgccatccg
     8941 cacgaattgt tttcagcaat tccaggccat ccatattggg catgttccag tcggagataa
     9001 caaatccata accgcctgcc tgcaacttat tgagagcgtc gacgccatct tccgcttcct
     9061 caacattatt gaatcccagc tctttcagca ggttacgcac tatgcgtcgc atggtggaaa
     9121 agtcatccac aaccaaaaat ttaagttctt tatccgccat ttcacactcc tgatttaaat
     9181 acgtatcgcc tgtccggcac taatttttgc caacatttgc tggcttacct ggctaagatc
     9241 gaccacttcg cagacaccac ccatattgat ggcctcgcgc ggcatgccga acaccacgca
     9301 acttgcttcg ttttgcgcaa gggtccatgc ccccgcctga cgcatcgcca acattcccgc
     9361 cgcgccgtcg ttgcccatac cggtcaggat caccccaacc gcattacgcc ccgcctgttt
     9421 ggcgacagaa tggaacaaca catctaccga aggccgatga cggttaaccg ccgggccatc
     9481 gtgaattttg atttggtaat ttgcgccact acgcgacagc tccatatgcc gatcgcccgg
     9541 cgcaatatag gcatgccccg gcaagacacg ttctccgtct tcggcttctt taaccccgat
     9601 ctggcaaagc ttattaagtc tgtcggcaaa agagcgggtg aaaccgggcg gcatatgttg
     9661 ggtaattaac aatgccgggc tggaaagcgg caacggttgc agtacgtgac gaattgcctc
     9721 agttccaccc gttgaagcac caatcgcaat cagtttttca gaactcaaca acggacccgc
     9781 cttcagcgtt gtcggtgctg acaatggctt atgtgctgca aggctcgcct ttgctgccgt
     9841 acgcaccttt tcagcaatca tttcgctata cgccagcatt ccttcgcgaa tacccagttg
     9901 cggtttggtg acaaaatcta ttgcccccag ctccagcgcg cgcagcgtga cttctgatcc
     9961 tttgccggtc aaggaagaaa ccatcacaac gggcattgga cgcaaacgca ttaatttttc
    10021 gaggaaatcc agtccgtcca tccgcggcat ttcaacatcc agcgtcagca catcgggatt
    10081 gaatttctta atcaagtcac gcgcgaccag cggatcaggc gcggtcgcca ccatttccat
    10141 gtcgctatgg ctgttgatga tttctgtcat gatctggcgc atcagtgccg aatcatcgac
    10201 agataacacc ctgattttgc tcatcgttaa tccttactta gcgcatacac cgtctgacca
    10261 cgcagcgtga agcggcgctc aaggtggcta aagttttcag agtgacccgc aaacagcaat
    10321 ccgtcgggtt taaggagcgg aacaaagcgg cgcaaaatct cctgctgggt attttgatcg
    10381 aagtagatca tgacgttacg acagaagatc gcatcaaacg gccccggcac ggtgtactgt
    10441 ttcgccagta aattcagcgg ggcaaaatca acatagttcg ccagctcctg acgcacgcgt
    10501 accagccctt catgcggtcc cgtccctcgc atgaaatacc gttgcagttg ctgcggcgtc
    10561 aggtttttca actcttcatg gcgataaata ccgcttctgg ctttttccag cacttcggtg
    10621 tcgatatcgc tggcaaacac tttccagcgt ccgggcgcgg tgcccaatgt gtcagccagc
    10681 gtcatcgcaa tgctgtatgg ctcttcgccg gttgaagccg ccgcgctcca tacgcgatac
    10741 tcgccagaac ggcgacgtgc gtgatccgcg agcagaggga aatgatgtgc ctcacggaaa
    10801 aatgccgtca gattcgtggt cagcgaattg ataaacgcct gccactcacc gctgtgctga
    10861 ttagattcca gcaagttcag ataatgaccg aaatccgtca gtcccagcga acgcaaacga
    10921 cgaaccagtc ggttgtaaac catgtcgcgt ttatgatcgg ccaggacgat cccggctcgt
    10981 tgatagatca attgacttat ccgccgaaaa tgcgcgtcgg aaagcgccag gcgctcggtc
    11041 atctgtaaca ataaagacgt ttgcccacag ggcagagatg aagtcatagc gccttctcaa
    11101 tcacttcagg ataccactgg cgcaatttgt aactgcgccg actcatgtcg tgctacttca
    11161 tgttcttcaa gagtaaatac cgccacacgc gacgaaagat ggtcggcctg attcgccagt
    11221 tgttccgttg ccaccgccgc ttcttctacc agcgaggcgt tctgctgagt cacctgatcc
    11281 atctggctga cagcctgtgc aacctgctca atcccccgct gttgttcttc cgacgcagag
    11341 gcaatttctc ccataatgtc gttcacgcgg gtcaccgaac tgacaatatc gatcatggtc
    11401 gcggcggcgt tattcaccag tttcgaaccc tgctggacac gattgactga ctcttcgatc
    11461 agccctttga tctcttttgc tgcctgcgcg ctacggctgg caagattgcg cacttcacct
    11521 gccactaccg caaaaccacg cccctgctct ccggcgcgag ccgcttccac tgccgcattc
    11581 agggccagaa tattggtctg gaaagcaatt ccgtcgataa cgctgataat gtcgccaatt
    11641 ttttgcgagc tggtggcgat ctcctgcatg gtgtgagtca tggtactgac ctgaacaccg
    11701 cccgccttcg ccgttgtcgc ggcatttttt gccagttccg acgcctgtcg tgcgttatcg
    11761 gcgttttgcc ctaccgtggc ggttaattgc tccatactgg cggccgtttg tgccagcgat
    11821 gccgcctgct gttcggtacg acttgagaga tcgttattgc ctgcgacaat ctccgcgata
    11881 ccaatgtgca tctcctggct tcccttacgc acatcactta ccgtcccacg caaagcctgc
    11941 tgcatggtct tcagactggc aaaaatggcg gtgatctcat tacgaccata taccgcaatc
    12001 ggacgcgcca gattacccgc agcaatgctg tcaaaatggc taccgataat ggccagtggt
    12061 tgaacaatca tcttgcgcgt ccaccacagc gcactgctga tgtagatcgc cgcaacaata
    12121 atcatgctga taaacaccag tgccgaaatc tgatagttac gctggctttg cgcactggcg
    12181 gcttccagca catggttgat ctccagctgc caggcctcaa agttaacgtc aaacgcattc
    12241 tgtgatccct gcaccggcgc agtgaggaaa tccgaaagtt gattactttc aagccaggtc
    12301 gcctgatgtt cgagatcgtt gtgccagcgg gcaaaacttt tctccgtctc tttttgcaat
    12361 gccctgacgt gctcgttgcc cgcagtcatc gccataaaac ttttaaacag cgtggtggat
    12421 tgcgtcagac tggcgcgcgc cgtcgtcatc aacgttttaa tgtcatccgc cggatagcta
    12481 agtgccgtca gagtgcccgc tttgttcagc gcggtactgg cctgtaacat taccgcccga
    12541 gtttgcgcta atgccgcacg ttgctgattg ctctgctcga cctgattcag tcgttgcaaa
    12601 tcgtcgcgaa atgcccaaaa agacatgccg ttactgccaa tctgcaagat cccgcagaga
    12661 atcaaaatta aaaacagcgt ggtcgaaatt cgaatacgat taaacatcaa cgctccccat
    12721 caggcggcaa tgaccgcgtt agtaaatact cgtcaaaatg tttcccagtt tggatcttgt
    12781 tcagcaattc gcagtcgtgg ctgagccggt ggttgctcac tggcaggacg ggatggtgtt
    12841 tgcggtttat tggtgagtgg gctggctgcc agacggaacg cggaaaccgc ttgcgttaaa
    12901 cgactcgcct gttcttccag cgcagcggcg gcggcagctg attcctgcac cagcgatgcg
    12961 ttctgttgcg tgacgcgatc catttccgaa accgccaatg cgacttgatc gatgccacgg
    13021 ctctgttcat ccgatgccga tgcaatctcg cccataatgt cagtcacgcg agtgacagca
    13081 ttgacgatat tgttcattgt ttccccggcg ctttcgacca gcaccgaacc ggtatcaacg
    13141 cgtgagacgg agtcttcaat gagggctttg atctcttttg ccgcttgggc gctgcgactg
    13201 gcaagattac gcacttcacc cgccaccacg gcaaaaccac ggccctgttc acccgcacgc
    13261 gcggcttcga ccgcggcatt cagcgcgagg atattagtct ggaaggcaat accgtcgata
    13321 acgctgataa tgtcggcaat tttcttcgaa ctatcggcga tctcatgcat cgttttcact
    13381 acgccatcca ccactttgcc gccgtgctgg gcggtgtcgg aggcactttg cgccagttgc
    13441 gaggcctggc gggcgttatc ggcgttttgc ttcactgtcg cggtgagctg ctccatgctg
    13501 gcggcagttt cttccagcgc ggatgcctgc tgttccgtac gggaggaaag atcggtgtta
    13561 cccgccgcaa tttcacgggt accggcatag atggcatctg aaccttcgcg cacatgagtg
    13621 acggtgtcag tcaaagagcg ttgcatatgt gaaacgctct gcgccaggtc gcccatttca
    13681 ctgcgcccgt caatggtcag ggtattagcc aggttaccac cggcgatttc gcgaatgtga
    13741 gcaataattt ttgccagcgg tgtaagcaac atacggcgaa tgccgtacca cgccaccagc
    13801 agaatcaata ccaccaccag cgcgataacc gccagttgcc actgggcaaa tcggtaatca
    13861 tctgcgttgt cagtgacgat atcgcgatac agtttttcac tgctgagggc gtactgagca
    13921 aacgcttcgc ccattgcatt ttgcattccc tgggttggct gagcgaaata agctccagta
    13981 ttgccataat caagataatc aatcagttca gttaacgctg tgtaatagtt tttatatttt
    14041 tcatcaatat tacgactggt agcgaccatt tcaggtaacg gtgccatgct tttgaatttt
    14101 ttataatgag tcgcggcctg ggccaatgtt ttcctggcgc tatcgagcaa ttcaactttg
    14161 gcgttacttt gttgattgga ggaatccatc atcatccgca ccgctgaacg actcaggtta
    14221 atgcgcgttt gcagcattaa atcccaggtt gacgtcagct cgccctgctg ttcccgtaat
    14281 tgattggaaa ccacaaagct cttctggcta tggtgaaggg aagaaaaaaa cagactgccg
    14341 gaaataagct gtaacagtgc gaataccccc agcaccatta ccaacagcgt gactacgcgg
    14401 atacggttaa tcataaggca ccttcctgaa aacaagttga tctcgttatc ggcaaggaag
    14461 ggggaaactt tattgctgat gccacccgcc gcgaaattga aataaaaaac ccgatgcgca
    14521 gatcatcggg ttcatttcaa ttgaggaaat agggagaatt acgccacttc tgacgccgcg
    14581 ctatctaaca gcgccatctc ttcgctgttc agcagttttt cgatgttcac cagaatcaac
    14641 atccggtcgc ccagtgcgcc cagtccagtg agatattctg ttgaaagcgt cacggcaaat
    14701 tccggtgccg gacgaatttg ctccgccgtc aatgaaagca catctgaaac gccgtcaacc
    14761 acgatgccga ccacccgctg tccgagattc aggacgataa ctaccgtgtt gtcgttatag
    14821 tccacatcca cctggctgaa cttaattcgt aagtcaacaa tcggcacaat aacgccgcgc
    14881 agattcgtga cgcctttgat aaacgctggt gtgttcgcaa tccgtgttac ctgatcgtag
    14941 ccgcggatct cctgcacttt caggatatca ataccgtact cttcatcacc aagggtaaat
    15001 accagaaatt cctggcctga cggctcgctg gccagctttg ttacattcgt cataccggtc
    15061 atattgttac ctttttactc attcaggcgg cggtgttcgc catacgttgt tcgcggttta
    15121 tcgcctgcaa ggcggagaca tcaacaatca gtgccacgct gccgtcgcca agaatggtcg
    15181 cagcagaaat gccggggact ttgcgatagt tactttcaag gtttttaact acaacctgat
    15241 gttgaccaat taattgatcc accagcaagg cgtagcggcg accgccactt tgtaagatca
    15301 ccacaattcc ctgggtagct tcggttttcg cgcccgcgac gttgaacact ttccacagtt
    15361 cgacgatggg cagatattca ccccgcactt ccagcacccg ctcgccgccg gccagtggat
    15421 ggagatcggc ttcacggggt tgcagtgatt ccataacagc attcagcggc agaatgaaaa
    15481 cttcatccgc aacgcgtacg gacatgccgt cgaggatggc cagcgtcagc ggcagtaaaa
    15541 tgcggatcgt agtgccagta ccctgcttcg actggatttc gacatgaccg cccatctcct
    15601 ggatattacg tttaacgacg tccatgccga cgccgcgccc ggagacgtcg gtgacctgct
    15661 ctgccgtgga gaagcctggt gcaaatatca gcatcgcgac ttcgtcgtcg ctcatgtttt
    15721 cgctgacagt caaaccttgc gaggccgctt ttgccagaat tcgctcacgg tttagccccg
    15781 ccccatcgtc ggtcacttca atgcaaatgt tgccgccctg atgttcggca gacagaatta
    15841 aatttccgac gctgttttta cctgcggcga gccgtttttc tggcagttca ataccgtgat
    15901 cgaggctatt gcgtaccagg tgggtcagcg ggtcgataat gcgttctatc aggctcttgt
    15961 cgagttcagt ggaactgccc accagcgtca gttctacctg cttgccgagt tttcccgcca
    16021 gatcacgcac cagccgggga tagcgactaa aaacatattc catcggcatc atgcgaatcg
    16081 acatcactga ttcctgcaaa tcacgggcgt tacgttgtaa ctgccccatg ctggtaatca
    16141 aatcaccatg attaaccggg tccagttcgc tggaacgctg ggcaagcatg gactgggtga
    16201 taaccagctc gccgacgagg ttaattaatt gatcaacctt ttctaccgct acacggatgc
    16261 tggtggattc actgctgcgc gtcgtttttt cccgctccac gcgaccggtt ggcgcttgtt
    16321 cggctgccag tttaagcact ggtggggtgg atatttttgg cgagacttct actgtttcaa
    16381 aggtaatctg atcggcttca atcacaaaac agagtaccgc tgtgatgtca tcttcggcga
    16441 tatcgcccgg taatattgcc gagagagaat ccgccccttt caccacgtca gttaacgttg
    16501 tcagatgccc cagctcttct tccagcaggt cgacttcgcc ggccttcagg cgcgaaagga
    16561 taattcgtcg cggcaactga ctgcgactct gctcatcttg cggttcactt ttggcaacca
    16621 cacttaatcg ggtcactgcg gatggcgttt cgcctttcgc ttctaatgcc agttgacgca
    16681 aggcctggca gatataatcg aagctggcgg catccggctc ttgcgactgt ttataagcgt
    16741 cgagctgttc ttgcatgatg tccttcgttt ccaaaaacag attgataatg tcggtgttga
    16801 gttgcatctc acctcgtctg gcttcatcga gcaggttttc catcagatgc gtggtttcct
    16861 gcaaaacgct gaagccaaaa gttcctgccc ctcctttgat cgagtgggca gcccgaaaga
    16921 tggcattcaa ttgttcggca tctggcgctt ccggctgcaa aaccagcaga tgctgctcca
    16981 tatcagccaa cagttcgtcc gcttcatcaa aaaatgtctg ataaaaatcg cttatatcca
    17041 tgctcacgct gtcacctcgg ttcggctgat ggcattgtgg gaacactgac ctgtggtgca
    17101 acctcaggtt tttccagggc gcttactggc tcattctggc tttcggcgtt ttcatgcaaa
    17161 atggcctgtt cggcttgttt gttcagtacc agcaggctga tgcgacggtt gacggcatca
    17221 tcaggtccgc gatcgcttaa gcgcatcgtt gccgccatgc cgaccacacg taacactttg
    17281 ccgctatcca accctccgac catcagttcg cggcgggatg cattggcccg atcggcagaa
    17341 agctcccagt tgctgtaacc tttctcacca ctggcgtagg ggaaatcatc ggtatgaccc
    17401 gaaaggctaa tacggttggg aataccattc agaacgggcg cgatggcacg cagaatgtcg
    17461 cgcatatagg gttcgacgtc ggcactgcca gttctaaaca tcgggcgatt ctggctatcg
    17521 atgatctgaa tacgtagacc ttcctggacc agatcgattt tgagatgtgg acgtaacgcc
    17581 cgcagtttcg gatcggactc tatcaattgg tcgagatcgc cacgcaattt ccgcaatcga
    17641 ctttgctcca tgcgtttttt caactcttcg atgttcggct gcttattcac ttccccctgg
    17701 ctttgggtgt aatcatcgcc accgccggga attgggcttt cactattaga aatgcgatcg
    17761 ccgcccgtaa ccgcagtcgc cagtggagtc cggaagtact ccgcaatctg aatcaactct
    17821 tttgggctgg agatggagat cagccacatc accagaaaaa aggccatcat cgcagtcata
    17881 aagtcggcat aagcaatctt ccacgatcca tgtgctgccc cgtggctttt ggctttgcgt
    17941 cgtttgacga caataatcgg atgcgcttga ttcttcatgc ttcctcggtt gtcgtctgtt
    18001 gttgcggatt tttcaccgca cgcacatgct cttccagttc aataaacgac ggacgttcgc
    18061 tggaatagag cgttttgcga ccaaactcaa cggcgatagg cggtgcgtaa ccgttcagat
    18121 tagaaagcag tgtgactttg acgcactgca tcattttgct ggtttcggcg cttttctgac
    18181 gtaaaacact cgctaatggg gaaataaatc cgtaagccaa taaaatgccg aggaaagtcc
    18241 ccaccatcgc atgtgcgata agcgccccca gctcggcggc aggacgatcg gctgaaccta
    18301 acgcgtgaac gacccccatt acagccgcga caataccaaa cgccggaagt gagtccccga
    18361 ccagcgccag actgttcgcc gggacttctg cctcgctttc gtgcgtctca atctcttcat
    18421 ccatcagggc ttcgatttcg aaggtgttca tgtgaccgct gataatcagg cgcagataat
    18481 cgacgataaa atcaagcatg acgctatccg cgagaatgcg tgggtagctg gcgaagatct
    18541 cgctctcacg gggattttca atatcacgtt ccagcgaaaa catccccatc tgccgcgatt
    18601 tcgccatcaa ccgataaagc agagccagca gatccatata cattgctttg gtgtatttgg
    18661 agcgacgaaa cagcaacggc agcgccttca gcgtgccttt aatcgctttg ccattattgc
    18721 cgacgataaa cgacccaatc cctgcaccgg caataatcac cagttcagcg ggttgataga
    18781 gtgctccaag gcttccaccg gtcatcaaat aaccgccgaa aactgtaccg agaacaacca
    18841 ggtaacctaa taagataagc acgacatcat ccttccactg ttgaccatga caggatgttc
    18901 agtcgtcagg cgttaacgcg cgattggggc aaaaaaaagc agcggtacgt cgttaccgct
    18961 gctggaatgt tgcaccacac cgtatcagtt aaacagcctg tactctctgt tcatccagca
    19021 gttgtgggat aatatcggca ggattctggg aaagtttacg tctttttact gcccgggatg
    19081 gcggttgaca taagctgcag gcaaagctgc caacaggctg gtgagcgtgg gtaataaaat
    19141 tgccgccgca gcagttgcag ctggaaagtt gcagtaatcc actttcaaca aaccgcacca
    19201 atgtccaggc acgggttaat gccagcagtg gtccttcttc tgcttgtggg cactgttcaa
    19261 ggtataaacg gtaggctttg atcaccgcat cgacgccatt acacaaaccg gttttcagta
    19321 aaaactgcca tgcattacag aacatcgaag catgaacgtt ttgttcccag gtcataaacc
    19381 agtcggttga gaatggcagc atgcctttcg gcggtgggct tccgcgcagt tctttataaa
    19441 gttttatcag gcgtccgcga cttaactgtg tttcgctttc cagcatctgc aaacgagcgc
    19501 ccagggtgat caattccatt gccagctgaa tatcccgcgc ttcctgaaca atgctttttt
    19561 cactcatgat caggcccttt tcttgcgcag cgcttcttca ggctgattaa catcattcag
    19621 caagcgtgtt gagagcatga tgccggtatg aatttgctgg agatcgtcaa cgcgggaatc
    19681 ttgcgtcaac tgagtaatcg tctggtggct gtcaaaacgg aagtgacaaa ccagttgatt
    19741 ggtttctgcc agcttaacca tttgcggaag agtcagtgcc gctaacgttg tcgccatttc
    19801 ttcatttatg ccgagacgaa acatagcgga cgctttgtcc tgaacaatca aacgctgtgc
    19861 aagtagtaaa tatgacaagt tgatgtcata aatgtgtttc agcaactcgg aggtatgcat
    19921 tattcccacc cagaataacc aactttattt ttatgcggtt tcaccgcacc ccgtgatgtc
    19981 gccgggaagc cccggtaaaa aataattagc attagaatag ttgcgataag ctgcaataag
    20041 cagaaccacc tttttggttt aatatgtcct tacaaataga aatgggtctt tacacttatc
    20101 taagattttt cctaaatcga cgcaactgta ctcgtcacta cacgcacata caacggaggg
    20161 gggctgcgat tttcaataat gtgtgatgca gatcacacaa aacactcaat tacttaacat
    20221 aaatgtataa gtcatacttt tgttttgggt gtatttcaat ctgttaaaaa gtttttcgct
    20281 acgctagcaa gcaaaaatga aacaggaata atcga
//
LOCUS       DAPGOF010000085        20060 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.085, whole genome shotgun sequence.
ACCESSION   DAPGOF010000085 DAPGOF010000000
VERSION     DAPGOF010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.085"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(74..286)
                     /gene="cspA"
                     /locus_tag="RHV13_002680"
     CDS             complement(74..286)
                     /gene="cspA"
                     /locus_tag="RHV13_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HDV1242202.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(567..857)
                     /locus_tag="RHV13_002681"
     CDS             complement(567..857)
                     /locus_tag="RHV13_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HDV1242203.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1291..2001
                     /locus_tag="RHV13_002682"
     CDS             1291..2001
                     /locus_tag="RHV13_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HDV1242204.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2051..3025)
                     /gene="ghrB"
                     /locus_tag="RHV13_002683"
     CDS             complement(2051..3025)
                     /gene="ghrB"
                     /locus_tag="RHV13_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HDV1242205.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3129..3788)
                     /locus_tag="RHV13_002684"
     CDS             complement(3129..3788)
                     /locus_tag="RHV13_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HDV1242206.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            3941..6274
                     /gene="bisC"
                     /locus_tag="RHV13_002685"
     CDS             3941..6274
                     /gene="bisC"
                     /locus_tag="RHV13_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HDV1242207.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRAAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6243..6683)
                     /locus_tag="RHV13_002686"
     CDS             complement(6243..6683)
                     /locus_tag="RHV13_002686"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDV1242208.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(6680..7243)
                     /gene="tag"
                     /locus_tag="RHV13_002687"
     CDS             complement(6680..7243)
                     /gene="tag"
                     /locus_tag="RHV13_002687"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDV1242209.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7401..8099
                     /locus_tag="RHV13_002688"
     CDS             7401..8099
                     /locus_tag="RHV13_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDV1242210.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8328..9536
                     /locus_tag="RHV13_002689"
     CDS             8328..9536
                     /locus_tag="RHV13_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1242211.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            9860..11551
                     /gene="eptB"
                     /locus_tag="RHV13_002690"
     CDS             9860..11551
                     /gene="eptB"
                     /locus_tag="RHV13_002690"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312452.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HDV1242212.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPVNAQ
                     AFAQLKKAADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAAAN"
     gene            11643..11719
                     /locus_tag="RHV13_002691"
     tRNA            11643..11719
                     /locus_tag="RHV13_002691"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11677..11679,aa:Pro,seq:cgg)
     gene            12464..14071
                     /gene="dppA"
                     /locus_tag="RHV13_002692"
     CDS             12464..14071
                     /gene="dppA"
                     /locus_tag="RHV13_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HDV1242213.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(14134..14361)
                     /locus_tag="RHV13_002693"
     CDS             complement(14134..14361)
                     /locus_tag="RHV13_002693"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242214.1"
                     /translation="MTRSLELRVCIAHKSIPALIGQGMKYCCRMALHAEAQHHRVCRL
                     LTKSALFMPDAALTPYPAYKIVQTQYIAEFS"
     gene            14379..15398
                     /gene="dppB"
                     /locus_tag="RHV13_002694"
     CDS             14379..15398
                     /gene="dppB"
                     /locus_tag="RHV13_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HDV1242215.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15408..16310
                     /gene="dppC"
                     /locus_tag="RHV13_002695"
     CDS             15408..16310
                     /gene="dppC"
                     /locus_tag="RHV13_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HDV1242216.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16321..17304
                     /gene="dppD"
                     /locus_tag="RHV13_002696"
     CDS             16321..17304
                     /gene="dppD"
                     /locus_tag="RHV13_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HDV1242217.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17301..18305
                     /gene="dppF"
                     /locus_tag="RHV13_002697"
     CDS             17301..18305
                     /gene="dppF"
                     /locus_tag="RHV13_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HDV1242218.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18335..19606)
                     /locus_tag="RHV13_002698"
     CDS             complement(18335..19606)
                     /locus_tag="RHV13_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HDV1242219.1"
                     /translation="MQHNTLPKHDQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVMVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         4783 a         4991 c         5299 g         4987 t
ORIGIN      
        1 tatttcctga aaaaaaataa aaaaatcccc gccaaatggc agggatctta gattctgtgc
       61 ttttaagcag agattacagg ctggttacgt taccagctgc cgggccttta gcgccgcttt
      121 cgatggtgaa ggacactttc tgaccttcgt ccagagattt gtaaccatcg ttctggatag
      181 cagagaagtg tacgaacaca tctttagagc catcgtcagg agtgatgaag ccgaagcctt
      241 tgtcagcgtt gaaccatttt acgataccag tcattttacc ggacatagtg tattaccttt
      301 aataattaag tgtgcctttc ggcgatatgg cgtgctttac agattttgaa gcgttaaagg
      361 aatgtgcact acgaggggta tcaacgataa ctcttgaagg gacttgcctt actacactgc
      421 tttaatggtc tgtacgtcaa accgttgatg tgcattaagc cacgcattgg cgggtgatgc
      481 aacaattatt tttcatattt atgattaatc ggttggaaaa cggtcctgtc atcaggaccg
      541 taaacagcaa taaagtggat aaaagtctat tccatcaact gcttacttaa tgccgggttg
      601 gcttgaatca aacgcatcaa ttttagttcg gcacttgaag gcttcacgcg tctggattcc
      661 cattccttta ccatggcgac tgatacgccc aaaacccggg cgaaatcatc gatttttaat
      721 cctgtccctt ttcgtaactg ctcaatttcg gtacaggacg ttgttctgtg cgtcagggta
      781 attttctgcg tttcatcttt aaaaacaatc tgttccaggc tgctcaacag ctcatgcatt
      841 ggatctttat attccattga aaactcctca aatcacactg cgggatcgtg aattgcatcg
      901 aagctcatta agaatagtcg ggaaaagcag acacagcggg gtgaagtcag ggcatgctct
      961 ggctatgatt cgattcagcc tgtcatttag cgtgacttat agccacctaa cgatctgata
     1021 aatgccgata tatctcttac cactcctgtt ttgtaaagat ttcgaaaatc taaccttctg
     1081 cgaaggcgta aatctctggc tacaaaaaag gcaacggcac tatcattttt taacaatgaa
     1141 acaacaaatt aaccttgttt cattttctcc atgattttac caggaagatt acgtagaaag
     1201 ggtatcctgc aagcctgtcc tgaactatcc ttgtcacgtc agacacgcgt gtgtcgttgt
     1261 gcgctttttt tgggtgaaag gagtaagaaa atggcgacag gaaagtcctg ctctcgctgg
     1321 tttgcgcctc ttgcggcgtt attaatggta gttagcctga gtgggtgttt tgataaagaa
     1381 ggcgatcagc gtaaagcgtt tatcgacttc ctgcagaata cagtgatgcg tagcggtgaa
     1441 cgtctaccaa ccctgactgc cgatcagaaa aaacagtttg gtccttttgt ctctgattac
     1501 gcgattctgt atggttattc tcagcaggtg aatcaggcga tggattccgg tctgcgcccg
     1561 gttgtagaca gcgttaacgc aattcgcgta ccgcaggatt acgttacgca aagcggtccg
     1621 ctgcgtgaaa tgaatggttc gctgggtgtg ctggcgcaac aactgcaaaa tgcgaagttg
     1681 caggccgatg ctgcgcactc tgcgctaaaa cagagtgatg acctgaaacc ggtctttgat
     1741 caggcattca ccaaagtggt aacgacgcca gctgatgcat tgcaaccgtt gattccggcg
     1801 gcacaaactt tcacgcaaca actagtgatg gttggagact acattgccca acaaggtact
     1861 caggtgagct ttgtagcaaa tggcatccag ttcccgactt cacagcaggc aagtgaatat
     1921 aacaaactga ttgcgccatt accggcacag catcaggcgt ttaatcaggc ctggactaca
     1981 gcagttactg caacccaata aagagtaaaa gcccgagcga ttatcgggct ttactccacg
     2041 cagtcgcggc ttagtccgcg acgtgcggat tcacacagtt cttctcaacc tttccttgta
     2101 acgcatcaat caaattatcc acggcacagg cggccatgcc ataacgcgtc tcatgggtgg
     2161 cagatccaat atgcggtact gcgacgacgt tggccattga gagcaacggc gaatctacgg
     2221 acagtggctc ttgttcgaag acatccagcc cggcggcgtg aatttcccct ttctgcaatg
     2281 ctgcgatcag tgcattttcg tcaaccaccg ggccacgtcc ggcattaatg aaaatggcgg
     2341 aggatttcat tttggcgaat tgttctgcgc caaacagatg atgcgtctca tcagttaacg
     2401 gcaggatcag gcaaacgaaa tctgactctt gtaacagagt atccaaatcg cagtagcggg
     2461 cgttgaagcg ttcttctgct tctttatggt ggcggcgcgc gttatagagg atgggcatgt
     2521 tgaagccaaa gtgcgcacgt tgtgccagcg ccataccgat ccgtcccatc ccgacaatgc
     2581 ccagtgtttt atggtgaacg tcagtgccgt accagtccgg gcctatgctc gcggtccatt
     2641 cgcctgcttt tacccgttct gctacctcca caacccgacg agcggtagac aacaccagcg
     2701 ccatcagcgt atcggcgacg gtttctgtta atacggttgg cgtgtgcatc agcagaattt
     2761 ttcgggcggt aagcgcatcg acatcaaaat tgtcatagcc gacggagatc gttgatgtgg
     2821 cacgcagttt cggcattttt tccagcaatg cggcatcaac attctcgttt gaacccagta
     2881 aaccttcagc ttcggcaaaa attgctgcat tttgttcgac ggtttgtggg ctgaggtttg
     2941 ccacctggtg aacggtgaaa tgctcttgca ggcgttgcag taaatcatca ggtaaggctt
     3001 tgtagaggat aacggacggc ttcatgcttc tctccattca ctgataaggc gatcacctta
     3061 gcctgaaata tgatgaagga aaactgaaga atgccaggtg atttctgcat cacctggcat
     3121 gaaagggatt acagcgggct taaggtaatt tctacacggc ggttttgcgc cttaccttct
     3181 gcggtgctgt tgctggcgat tgggttagcc gggccaaggc cctgagtacg gatgcggctg
     3241 gcgtccacgc cctgggtgat caacgcgctg gcaacggaat ccgcacgttg ctgggagaga
     3301 cgcatgttca ggtcgtgacc acccgtgctg tcagtataac caatcacgtt aaccgccgtt
     3361 ttcggatact ctttcagcac cattgccacg ccggtcaggg tgttagcgcc cgccggtttc
     3421 agggtcgcgc tgctgctgtc gaaggtcaca ttgttcggca tattgaggat aatgttatcc
     3481 ccgctgcggg ttacgctaac accagtgccg cgcattttgt cacgcagctt cgcttcctgc
     3541 acatccatgt aataacccac gccgccgccc agagctgcgc ctgctgctgc gccaatcagc
     3601 gcgcctttac cgcgatcttt cttagaagaa gagagcgctc caatacccgc gcccacgaga
     3661 gagcccagac ctgcgccgat agcagattta cctgcttcgc gttcgccggt gtaagggtta
     3721 gttgtgcagc cagataccgc cagagcgcca ctcactacgg cggcaataag ataaacacgt
     3781 ttcttcattg ttaatcctta ataacctttt tattctttgc cacgggttcc gtggcgggag
     3841 attatgccgc gtgaacatga agattattcc tgggaatact cggaaatttg taagtaatat
     3901 ttaactgctc aatacatcta acctttcagg agtcttcggt ttggccaact catcctcacg
     3961 atattccgtt ctgactgccg cccattgggg gcccatgctg gttgaaaccg acggcgaaac
     4021 cgtgtttagc tcgcgtggcg cgttagccac aggaatggaa aactccttgc agagcgcggt
     4081 tcgcgaccag gttcacagca atacgcgggt acgatttcca atggtgcgca aaggctttct
     4141 cgcgtcaccg gaaaacccgc aaggcattcg tgggcaggat gaatttgttc gcgtgagttg
     4201 ggatgaggcg ctggatctta ttcaccaaca acataaacgc attcgtgagg cttatggtcc
     4261 ggcatcgatt tttgctggtt cctacggctg gcgttcaaac ggcgtgctgc ataaggcctc
     4321 gacattatta caacgctata tggcgctggc aggcggttat accgggcatc tgggggatta
     4381 ttcgaccggt gcggcacagg cgatcatgcc gtatgtcgta ggtggcagcg aagtttatca
     4441 acagcaaacc agttggccgc tggtgctgga acatagcgat gtcgtggtgt tgtggagtgc
     4501 taatccactc aatacgctga aaattgcgtg gaatgcatcc gatgagcagg ggctttctta
     4561 cttttccgca ctgcgtgaca gtgggaaaaa gctgatctgc attgatccaa tgcgatcgga
     4621 aaccgtcgat ttctttggcg ataaaatgga gtgggtggca ccgcacatgg gcaccgatgt
     4681 tgcgctgatg ctggggatcg cccatacgct ggtggaaaat ggttggcacg acgaagcgtt
     4741 tctggcgcgt tgcaccacag gttatgccgt cttcgcctct tatttgctgg gcgagagtga
     4801 cggaatagcg aaaaccgccg aatgggcagc agagatttgt ggtgttggcg cagcgaaaat
     4861 ccgcgagctg gcggctattt tccaccaaaa taccaccatg ctgatggctg gctggggaat
     4921 gcagcgccaa cagtttggtg agcaaaaaca ctggatgatc gtcacgctgg cagcaatgtt
     4981 ggggcaaatc ggcacacccg gcggcggttt tggtctttct taccattttg ccaatggtgg
     5041 taaccccacg cggcgcgctg cggtactctc ttccatgcag ggcagcttgc cgggtggctg
     5101 cgatgcggta gataaaatcc ctgtcgcccg cattgttgaa gcactggaaa accccggtgg
     5161 cgcatatcaa cacaacggta tggaccgaca tttcccggat attcgtttta tctggtgggc
     5221 gggcggtgcc aattttacac atcatcagga tactaatcgc ctgatccgtg cctggcaaaa
     5281 accggagctg gtggtgatct ctgaatgttt ctggacggcg gcggcgaaac acgcggatat
     5341 cgttctgcct gcgaccacct cgtttgagcg taatgatctc accatgaccg gcgattacag
     5401 taatcagcat ctggtgccca tgaagcaagt ggtgccgcca cgctatgaag cgcgtaatga
     5461 ttttgatgtt tttgccgagt taagtgaacg ctgggagaag ggcggttatg cacgttttac
     5521 ggaaggaaaa agtgagctgc aatggctgga aacgttttat aacgttgccc gacagcgcgg
     5581 ggcaagccag caggttgaat tgccgccatt tgctgagttc tggcaagcca accagttaat
     5641 tgagatgccg gaaaacccgg acagcgagcg gtttattcgc ttcgctgatt tttgccgcga
     5701 tccgctggcg catccgttaa aaaccgccag cggcaagatt gaaatcttct cacagcgtat
     5761 tgccgattac ggttacccgg attgccctgg gcatccaatg tggctggagc cggacgaatg
     5821 gcagggcaat gccgaaccag aacagttgca ggtactttct gcccatccgg cgcaccgcct
     5881 gcacagccag ctgaattaca gttctctgcg cgaattgtac gcggtagcaa accgtgagcc
     5941 tgtcaccatt catcctgacg atgctcaggc gcgcggcata accgaagggg atatggtgcg
     6001 ggtgtggaac tcccgtgggc agatccttgc cggagcggtc attagcgagg gaattaaacc
     6061 tggcgtgatt tgcattcacg aaggggcatg gccggatctg gatttaaccg ctgacggtat
     6121 ttgtaaaaac ggcgcagtga acgtgctgac caaagatctc cccagctcgc ggctggggaa
     6181 tggctgtgcg ggtaatacgg cgctggcatg gctggaaaaa tacaacggtc cggaactgac
     6241 acttacagcg tttgaaccac cggccagctc ataatccagg tgggtagttg ggtttcatcc
     6301 tgccatgcgc aatcgacaat gtgaaaaccc tgcgcacggt aaaaatctat cgccggttga
     6361 tttttttgat aaacctccag catcagatgg ggatagcgct gctgcacata ctgcatcagc
     6421 gccttaccaa taccgcgtct gacggccttc ggtgcgacaa acattgctgc cagaaatcgg
     6481 ccttccataa tgctgacaaa accgagaagc ttaccgtcct cttcccagac ccagttttgc
     6541 gcgttggcaa gataggcatc ccgcaccagc ggaatgcagt cacgccagta attcgctttt
     6601 ataaagggat gcccccaggt tgtactttcc agccacagtt cgaggatcgc ggggagttct
     6661 gaacgttgcg cttcccgaat catggtttat ttcccggata gcaacagcag ccaaccacat
     6721 gatcattcac cagcccacat gcctgcataa aggagtaaca gattgtggtg ccgacaaact
     6781 taaaaccacg ttttttcagt gccttagata gggcgtcgga ggcggacgta gatgtgggaa
     6841 tttcgctcaa cgttgtggct tgtgtcacct gtggctgatg atttacaaac gaccagacaa
     6901 agtcggcaaa cggttcgccg ttctgttcca tttgcaggta cgcccgcgca ttaccaataa
     6961 ttgcctgaat tttccctcga tggcggataa tcccggcgtc ctgtaccagt ctttcgacat
     7021 cctcttcctg cattgctgcg accttcaccg gatcgaactg atgaaagcag gcgcgatagt
     7081 tttcgcgttt tttgaggacg gtgatccacg ataacccagc ctgctgccct tcaaggcaga
     7141 tcatttcgaa cagtttttta ctgtcagttt caggcacgcc ccactcatta tcatggtagg
     7201 caatataaag cgggtcctga ctcacccagc cgcaacgttc catactttcc ctcgctatgt
     7261 acccgatcgt aaaaatttca gccgcttttc ttgacgcatc ggtgaaaaag tcaatattta
     7321 atacagggct actatgcccg atatccttca tacaatgaca ataatattgc cgaattcggc
     7381 tcttccctgg agtcgtgtta atgatcataa aaaaaagcgg tggtcgctgg cagctaagcc
     7441 tgctggcgag cgtggtaatc agtgcctttt ttctcaacac agcttacgcc tggcaacaag
     7501 aatatatcgt tgatacgcaa cccggacatt ccacagagcg ttacacctgg gatagtgatc
     7561 atcaacctga ttacaacgat attttgtcgc aacgtattca aagtagccaa agggcgctgg
     7621 gactggaagt caatctggcg gaagaaactc ctgtggatgt gaccagcagt atgagtatgg
     7681 gctggaattt tcctttatat gaacaggtta caaccggccc ggtcgcggca ttacattacg
     7741 atggcacaac cacctcgatg tataacgagt ttggcgacag tactaccacg ctgaccgatc
     7801 cgttatggca tgccagcgtg agtagcttag gctggcgtgt tgactcccgg cttggcgatc
     7861 tccgaccctg ggcgcaaatc agctataacc agcaatttgg cgagaatatc tggaaggcgc
     7921 aatcaggcct gagccggatg acggcgacaa accagaacgg caactggctg gatgtcaccg
     7981 taggcgctga tatgttgctc aatcaaaata ttgccgccta tgccgcgcta actcaggcag
     8041 aaaataccac taataatagc gactatctgt atacgatggg ggttagcgcc agattttaac
     8101 gtaacagtca caattgaaac cattgaataa caacagttgt ggcgatagtg ggtgctaact
     8161 taccaaataa taaatttggt gaataattgt cgcgtcattc attcctgaac taaggcattt
     8221 cattccgttc tgatggcatt tcatgccgtt tttccccagg cataaaatgc acttcgttat
     8281 ggttgtcggc agagattttt cctttttatt actgcaggaa tactgccatg acaccttcaa
     8341 attatcagcg tacccgctgg ctgacactca tcggtactat cattacccag tttgcactgg
     8401 ggtcggttta tacctggagc ctgtttaatg gcgcgctttc tgccaagctg gatgcgccgg
     8461 taagccaggt cgctttctct ttcggcttgt taagtctggg gctggcaatt tcgtcttctg
     8521 ttgcgggcaa attgcaggaa cgttttggcg ttaaacgcgt caccatggct tccggcattt
     8581 tgctgggatt aggcttcttc ctgaccgcgc attccaacaa cctgatgatg ctgtggttaa
     8641 gcgccggtgt gctggtcggt ctggcggatg gcgcgggtta cctgctgacg ctctctaact
     8701 gcgtgaagtg gttcccggag cgtaaaggtc tgatatccgc gttcgctatc ggttcttatg
     8761 gtctgggtag cctgggtttc aaatttatcg acacgcagct gctggaaacg gtgggtctgg
     8821 aaaaaacctt tgtgatttgg ggagcgattg cgctggtgat gattgttttc ggcgcaacgt
     8881 taatgaaaga cgcaccaaaa caggaagtga aaaccagcaa tggtgtggtg gagaaagatt
     8941 acacgctggc agagtcgatg cgtaaaccgc agtactggat gttagcggta atgttcctga
     9001 ctgcctgcat gagcggcctg tacgtgattg gggtagcgaa agatatcgcc caaagtctgg
     9061 cacaccttga tgtggtttcc gcagccaatg cagtaacggt tatttccatc gctaaccttt
     9121 caggtcgtct ggtgctgggc attctgtctg acaaaattgc ccgtatccgt gttatcacca
     9181 ttggtcaggt gatttcgctg gtgggtatgg cggccctgct gtttgcacca ttaaatgcag
     9241 tgacgttctt tgcagcgatt gcctgcgtgg catttaactt tggcggcact attaccgtct
     9301 ttccatcact ggtcagtgag ttcttcggcc tcaataacct ggcgaaaaac tacggtgtga
     9361 tttacctcgg tttcggtatc ggtagcattt ttggttcgat tatcgcctca ctgtttggcg
     9421 gtttctatgt gaccttctac gtgattttcg ccctgctgat tctgtcattg gcgctttcta
     9481 cgacgattcg tcagccagag cagaaaatgt tgcgtgaggc gcatggctcc ctttaatgct
     9541 cagccgcgag ctactgggct tcagcttccg tgtgcatcaa acgcatcaac gaaatgtata
     9601 aacaatgact cgtaaaattt gggccagtta actggccctt tttactgctt tttgtaacgc
     9661 taaagtctct tttcaaactt gcatttttgt aaatttgtgc ttcatgcaca ctctttcccc
     9721 acactttttc gctttgctgt ggtctactta ttcgcgcgtg tagattttac ttatctgact
     9781 acctccgcac ttttcccctg ccgggcctga aaagccgcta agcagggtgt tatcacctgt
     9841 ttgtccaggg tttgtttgca tgagatacat caaatcgatt acacagcaga agctgagctt
     9901 tttgcttgca atctatattg gcctttttat gaatggcgcg gttttttatc gccgcttcgg
     9961 cagctatgcg cacgatttta ccgtctggaa aggcgtttct gctgttgttg aactggccgc
    10021 caccgtactg gtgaccttct ttttactacg tcttctatcg ctgtttggcc gccgcagctg
    10081 gcgtattctg gcgtcgctgg tggtgctctt ttccgcaggt gccagctatt acatgacctt
    10141 ccttaatgtg gtcattggtt atggcatcat cgcttccgtc atgaccaccg atatcgacct
    10201 gtcaaaagaa gttgttggtc tgaactttat tctctggtta atcgccgtta gtgcattgcc
    10261 tcttatcctt atctggaata accgctgtcg ctacaccttg ctccgacaac tgcgaacccc
    10321 agggcagcgt attcgcagcc tggcggtcgt cgtactggcg ggtattatgg tttgggcacc
    10381 gattcgtttg ctggatatcc agcagaagaa agtggagagg gcgaccggcg ttgatttgcc
    10441 gagttatggc ggtgtcgtag cgaactctta tctgccatca aactggcttt ctgcgttggg
    10501 gctgtatgcc tgggcgcggg tcgatgaatc ttccgataat aattcattgc ttaatccggc
    10561 gaagaaattc acttatcagg caccgcaaaa cgttgatgac acttatgtcg tgtttatcat
    10621 cggtgaaacc acgcgttggg accatatggg cattttcggc tatgagcgta ataccacgcc
    10681 gaaactggcc caggagaaaa atctggcggc gttccgtggt tactcctgtg ataccgcaac
    10741 caaactctca ctgcgttgca tgtttgtacg tcaggggggc gcggaagata atccgcagcg
    10801 cacattaaaa gaacagaaca ttttcgcggt tctgaagcag ttaggattca gttctgacct
    10861 ctacgctatg cagagcgaaa tgtggttcta cagcaacacg atggcggaca acattgctta
    10921 tcgtgagcag attggtgcgg agccacgtaa ccgtggcaag ccggtagatg atatgttgct
    10981 ggtagacgaa atgcagcaat cgctggggcg caacccggat ggtaagcatc tgatcattct
    11041 gcataccaaa ggttcgcact ttaactacac ccagcgttac ccgcgcagct ttgcgcagtg
    11101 gaagccggaa tgtattggtg ttgatagcgg atgtaccaaa gcgcagatga tcaactccta
    11161 tgacaactcg gtgacctatg tggatcactt tatctccagt gtgattgatc aggttcgcga
    11221 taagaaagcg attgtgttct acgcagctga ccacggcgag tcaattaatg aacgcgagca
    11281 cctgcacggc acgccgcgtg aactggcacc gccggagcag ttccgcgtac cgatgatggt
    11341 ctggatgtca gataaatatc tggaaaatcc ggtcaatgcg caggcgtttg cgcagctgaa
    11401 aaaagcagcc gacatgaaag tgccacgccg tcacgtagag ctgtacgaca ccatcatggg
    11461 ttgtcttggc tatacttcac cggatggtgg aattaacgaa aacaacaact ggtgtcacat
    11521 cccgcagaca aaagaggcag cggctaacta agaggcctgc tgactttctc gccgatcaaa
    11581 aggcattttg ctattaaggg attgacgaag gcgtatctgc gcagtaagat gcgccccgca
    11641 ttcggtgatt ggcgcagcct ggtagcgcac ttcgttcggg acgaaggggt cggaggttcg
    11701 aatcctctat caccgaccaa tttagaaaaa ccagcctacg ggctggtttt ttgctttctg
    11761 cgacctgaag aggatgagaa cctccggggg cagggaggtt cgactcgacg taggcctgat
    11821 aagacgcgtc agcgtcgcat caggcaacgg caccgcactt attgtcggat gcggcgtgaa
    11881 cgccttattc gacctacacg ccacacattt ccctgccaaa tttgcgcccc atatctcact
    11941 atccattaac ggatttgtga cagaaaccat tgtctttttt tatatttgct taaatctttt
    12001 tttgcgttac ttatggctgg cgctggcatt ttccgctgtg cgttttagcg ttcatggtat
    12061 taatcgctca gatatccacc ttttcctcag atttttttca ctaaatgcat aaaacttaat
    12121 caccaaatgt tgctaaatat taagctgatt gttctggtca tgttggagta gcacaaattt
    12181 ctctgctgca aatagctgtt tgaagttttt ttaatctttg tttgtggatt ctcaccgttg
    12241 gtgtaaatcc cggttggctg tattgacgtt ttcacattct gttgacagat tgtaggtcac
    12301 gaggggcatt ttatggagga tccgcactgt tacactgatg ttaattagta cggcatcccc
    12361 acctcataac gttgacccga ccgggcaaaa aacaaaaaag gtcaggcagc gacaacccac
    12421 tgcaaagggt taaaacaaca aacatcacaa ttggagcaga ataatgcgta tttccttgaa
    12481 aaagtcaggg atgctgaagc ttggtctcag cctggtggct atgaccgtcg cagcaagtgt
    12541 tcaggctaaa actctggttt attgctcaga aggatctccg gaagggttta acccgcagct
    12601 gtttacctcc ggtaccacct atgacgcctc ttccgtaccg ctttataacc gcctggttga
    12661 atttaaaatc ggcaccactg aagtgatccc gggcctcgct gaaaagtggg aagtcagcga
    12721 agacggtaaa acctatacct tccatctgcg taaaggtgtg aagtggcacg acaataaaga
    12781 attcaaaccg acgcgtgaac tgaacgccga tgacgtggtg ttctcgttcg accgtcagaa
    12841 aaacgcgcaa aacccgtacc ataaagtatc tggcggcagc tacgaatact tcgaaggcat
    12901 gggcttgcca gagctgatca gtgaagtgaa aaaggtggac gataacaccg ttcagtttgt
    12961 gctgactcga ccggaagcgc cgttcctcgc tgaccttgca atggacttcg cctctattct
    13021 gtcaaaagaa tatgctgatg cgatgatgaa agctggtaca ccggaaaaac tggacctcaa
    13081 cccaatcgga accggtccgt tccagttaca gcagtatcaa aaagattccc gtatccgcta
    13141 caaagcgttt gatggctact ggggcaccaa accgcagatc gatacgctgg ttttctctat
    13201 tacccctgac gcttccgtgc gttacgcgaa attgcagaag aatgaatgcc aggtgatgcc
    13261 gtacccgaac ccggcagata tcgcccgcat gaagcaggat aaatctatca acctgatgga
    13321 aatgccgggg ctgaacgtcg gttatctctc gtataacgtg cagaaaaaac cacttgatga
    13381 cgtgaaagtt cgccaggctc tgacctacgc ggtgaacaaa gacgcgatca tcaaagcggt
    13441 ttatcagggc gcgggtgtat cagcgaaaaa cctgatcccg ccaaccatgt ggggctataa
    13501 cgacgacgtt caggactaca cctacgatcc tgaaaaagcg aaagccttgc tgaaagaagc
    13561 gggtctggaa aaaggtttct ccatcgacct gtgggcgatg ccggtacaac gtccgtataa
    13621 cccgaacgct cgccgcatgg cggagatgat tcaggcagac tgggcgaaag tcggcgtgca
    13681 ggccaaaatt gtcacctacg aatggggtga gtacctcaag cgtgcgaaag atggcgagca
    13741 ccagacggta atgatgggct ggactggcga taacggggat ccggataact tcttcgccac
    13801 cctgttcagc tgcgccgcct ctgaacaagg ctccaactac tcaaaatggt gctacaaacc
    13861 gtttgaagat ctgattcaac cggcgcgtgc taccgacgac cataacaaac gcgttgaact
    13921 gtacaagcaa gcgcaggtgg tgatgcacga tcaggctccg gcactgatca tcgctcactc
    13981 caccgtgttt gaaccggtac gcaaagaagt taaaggctat gtggttgatc cattaggcaa
    14041 acatcacttc gaaaacgtct ctatcgaata attaaaagcc atacaagact gatggcaaag
    14101 gcaaaactgc ctgatgcgct ccgcttatca ggcctacgaa aattctgcaa tgtattgagt
    14161 ttgcacgatt ttgtaggccg gataaggcgt taacgccgca tccggcataa acaaagcgct
    14221 ctttgtcaac aatctgcata cccggtggtg ctgtgcctct gcgtgcagcg ccatccggca
    14281 gcaatacttc attccctgtc cgataagggc gggaattgat ttgtgagcaa tacagacacg
    14341 cagttccagg ctgcgggtca ctatagagaa tccgggttat gttgcagttt attctccgac
    14401 gtttgggact cgtcatcccc acgtttatcg gtattaccct tctcacattt gcctttgtcc
    14461 acatgatccc gggcgatccg gtgatgatca tggcgggcga acgtgggatc tccccagagc
    14521 gtcacgcgca gctgctggct gaactcggct tagataaacc gatgtggcag cagtatctcc
    14581 attacatttg gggcgttatg catggcgatc taggcatttc aatgaaaagc cgcatcccgg
    14641 tttgggaaga gttcgtgccg cgcttccagg ccacgctgga acttggtgtc tgcgcgatga
    14701 tttttgctac ggcagtcggt attccggttg gtgtgctggc tgcggttaaa cgcggttcca
    14761 ttttcgatca cactgcggtt ggcctggcac tgaccggcta ctcgatgccg atcttctggt
    14821 ggggcatgat gctgatcatg ctggtttcgg tgcactggaa cctgacgccc gtctccggtc
    14881 gtgtgagtga catggtattc ctcgatgact ccaatccgtt aaccggtttt atgctgatcg
    14941 acaccgccat ctggggtgaa gacggcaact ttatcgatgc cgtcgcccat atgatcttgc
    15001 ccgccattgt gctgggtacc atcccgctgg cggtcattgt gcgtatgacg cgctcctcga
    15061 tgctggaagt gctgggtgaa gattacatcc gcaccgcgcg cgccaaaggg ttgacccgca
    15121 tgcgggtgat tatcgtccac gcactgcgta acgcgatgct gccagtagtg accgttattg
    15181 gtctccaggt gggaacattg ctggcagggg cgattctgac cgaaaccatc ttctcgtggc
    15241 ccggtctggg gcgctggttg attgacgcgc tgcaacgccg cgattatccg gtggttcagg
    15301 gcggcgtatt gctggtggcg acgatgatta tcctcgtcaa cctgctggtc gatctgctgt
    15361 acggcgtggt gaacccgcgt attcgtcata agaagtaagg ggacatcatg tcacaggtta
    15421 ctgaaaataa agtgattagc gcaccggtgc cgatgacccc gttacaggag ttctggcact
    15481 attttaaacg caataaaggc gcggttgtcg ggctggttta cgtcgtcatc gtgctgttta
    15541 tcgcgatctt tgccaactgg attgcaccct ataacccggc ggaacagttc cgcgatgcac
    15601 tgctcgcccc gccagcctgg caggaaggcg gcagcatggc gcacttgctg ggcaccgatg
    15661 acgtaggccg tgatgtgctg tcgcggctga tgtacggcgc gcgtttatcg ctgctggttg
    15721 gctgtctggt ggtggtgtta tcgctgatta tgggcgttat tctcggcctg atcgccggtt
    15781 actttggcgg cctggtcgat aacatcatca tgcgcgtggt cgatatcatg ctggcgctgc
    15841 caagtctgct gcttgcgctg gtgctggtgg caattttcgg cccgtcgatt ggtaacgccg
    15901 cgttggcact gaccttcgtt gccttgccgc actatgtgcg cttaacccgt gccgccgtgc
    15961 tggtggaagt taaccgcgat tacgtcaccg cgtctcgcgt ggcgggtgcc ggggcgatgc
    16021 gccagatgtt tattaacatc ttcccgaact gccttgcgcc gctgattgtt caggcgtcgc
    16081 tcggtttctc taacgccatt ctcgatatgg ctgctcttgg tttcctcggc atgggggcgc
    16141 agccgccaac gccggagtgg ggcaccatgc tctccgacgt gttgcagttc gcgcaaagcg
    16201 cctggtgggt cgtgaccttc ccgggtctgg cgatcctgct gacggtgctg gcatttaacc
    16261 tgatgggtga cggtctgcgt gacgcgctcg atcccaaact gaagcagtaa gaggttcgag
    16321 atggcgttat taaatgtaga taaattatcg gtgcatttcg gcgacgaaag cgcaccgttc
    16381 cgcgccgtag accgcatcag ctacagcgta aaacagggcg aagtggtcgg gattgtgggt
    16441 gagtctggct ccggtaagtc ggtcagttca ctggcgatta tggggctgat tgattatccg
    16501 ggccgcgtaa tggcggaaaa gctggtgttt aacggtcagg atttgcagcg tatatcagaa
    16561 aaagagcgcc gcaacctggt gggtgccgaa gtggcgatga tcttccagga cccgatgacc
    16621 agccttaacc cgtgctacac cgtgggtttc cagattatgg aagcgattaa ggtgcatcag
    16681 ggcggtaata agagcactcg ccgtcagcgg gcgattgacc tgctgaatca ggtcggtatt
    16741 cccgatccgg catcgcgtct ggatgtttac ccgcatcagc tttccggcgg catgagccag
    16801 cgcgtgatga ttgcgatggc cattgcctgt cggccaaaac tgctgattgc cgatgaacca
    16861 accactgcgc tggacgtgac cattcaggcg caaatcattg aactactgct ggagctacag
    16921 cagaaagaga acatggcgct ggtgttgatc actcatgacc tggcgctggt ggcagaagcg
    16981 gcacataaaa tcatcgtgat gtatgcaggc caggtggtgg aaacgggcga tgctcacgcc
    17041 atcttccatg cgccgcgtca tccgtatact caggcattgc tgcgcgcgct gccggaattt
    17101 gctcaggaca aagaacgtct ggcatcgttg ccaggtgtcg ttcccggcaa gtacgaccgc
    17161 ccgaacggct gcctgcttaa cccgcgctgc ccctatgcca ctgacagatg tcgcgctgaa
    17221 gaaccggcgc tgaatatgct cgctgatggg cgtcagtcca aatgccatta cccacttgat
    17281 gatgccggga ggccgacact atgagtacgc aagaggccac ctcgcaacaa ccgctgttgc
    17341 aggctatcga cctgaaaaaa cattatccgg tgaagaaagg tatgttcgct ccggaacgtc
    17401 tggtgaaggc gctggacggc gtttcgttta accttgaacg tggcaaaacg ctggcggtag
    17461 taggtgaatc tggctgcggt aaatcgaccc tcggtcggtt gctgacgatg attgaaacgc
    17521 ccaccggtgg tgagctgtat taccagggcc aggatctgct taagcacgat ccgcaggcgc
    17581 agaagctgcg tcggcagaaa atccagatcg tcttccagaa cccttacggt tcgctgaatc
    17641 cgcgtaaaaa agtcgggcaa attcttgaag agccgctgct gatcaacacc agcttaagca
    17701 aagaacagcg tcgggaaaaa gccctgtcga tgatggcgaa agtcggcctg aaaaccgagc
    17761 actatgaccg ctatccgcat atgttctccg gcggtcagcg tcagcgtatc gccattgccc
    17821 gaggtctgat gctcgacccg gatgtggtga ttgccgatga gccggtctcc gcgctggacg
    17881 tctcggtgcg tgcacaggtg ctcaacctga tgatggattt gcagcaggaa ctggggcttt
    17941 cttatgtctt catctcccac gacctgtcgg ttgtggagca cattgctgat gaagtgatgg
    18001 tgatgtacct gggccgctgc gtggagaagg gaacgaaaga ccaaatcttc aataacccgc
    18061 gccatccgta cactcaggcg ctactttccg cgacgccgcg cctgaatccg gacgatcgcc
    18121 gcgagcgcat caaactcacc ggtgaactgc caagcccgct gaatccaccg ccgggctgcg
    18181 cgtttaacgc ccgctgtcgt cggcgcttcg gcccctgcac ccagttgcag ccgcagctaa
    18241 aagactacgg cggtcaactg gtagcttgtt ttgctgttga tcaggatgaa aatccgcagc
    18301 gttaactcac aaaaccgggg ccatgcgctc cggtttactc aatcagcttc aacagtggcg
    18361 acaagcatag caaaatgccg taaagcagaa tcagccaggc tttaaatccg cgaagttttt
    18421 ccagttgtgc gactttatag atcaggaaaa acggaatgag gcacgacaca atgccatata
    18481 acgggcttcc caactgaaag aacaccagca ccgatacacg aaacgaaacc caaatcgtca
    18541 acgtaatgac gataaaagcg cagatcgcca gagtcagcac gcgtgagtta attttcttgg
    18601 tatcaataat tcggcttaac agattgagaa taatgccttt aatggcctcg tggaaaccga
    18661 gataaatgcc aaagaatgcg gtcagtacgg caaagatatt cagcaccgta gaggtgatat
    18721 gaatgatatg cccagggatc acctgcgcgg ccagtgccag tgccgagata ttttgttcaa
    18781 aggcagaaac ggcttcttcg tggctaattg agaaggtaaa cgaaaaggca aaaaacagta
    18841 tcaccgcgat gagcgtgata taactaattc ggtgggtacg cagcgcgagc cgggttgcca
    18901 gtactttatc cgcttcccgt ttacgatagg caatattcat tgggttaagt acctgaataa
    18961 atactgcaga aaagaagcaa aatggaatgg taagcaggac atcgcgaaaa aagacggagg
    19021 cttgcgggaa ggcggttata ttggcgaaat tccagtgcgg gatcatcgca aaaccgaaca
    19081 cgacaataat ccctactttg accatcacca ttggcccaga aatcttaaac agtaatcgtt
    19141 caccaccaga cgcaatcgct accagtacgg cgaaaatagc gactttataa agtagagatt
    19201 gtgaaagatc ggcatcggtt aaaccgaagg ttttcaggta cgaggcgctg tcgaaaacca
    19261 cggagagaga gtagataaaa atcccgtgga taatcatcaa aaagtagata accccgagga
    19321 aaattcccca gttcttcccc aggtaatgac tgataatatc ggtgtagtca ttacaggaat
    19381 cgctttcaga aagggttttt aaataaatgt cctgcactac ccaggtggca ggataagcaa
    19441 tgatcgctgc ggtaataaat acccaaattc ccttcaagcc aatttgtact ggcatcagca
    19501 cggttccggc accaatcgcc atgcctatgc ataataaaac ccagccgaag tcgtagcgtg
    19561 taaacggcaa tttctgatcg tgtttcggta gtgtgttgtg ctgcataaag tcctgtccag
    19621 aaagaagtcg gcctcgcaaa aatgaataaa cccagtttct cttgcgcaac aggtggagaa
    19681 gtcgaatacg cagtatgtaa aaaatgaggc aacagattca ctggggaggt cgcgaggagg
    19741 cgcaattcta caaaattgtg catttcacta ccagtctgga gtcagcccgg atatgaactg
    19801 caactattgc aaattaagaa attaagctgg aaggcatcac gcaatgagga agattgccgg
    19861 gtgctgacag taaaaagcaa tcgtaaaatc agcggcaagc cggaagggtt ccggtgaggc
    19921 gcaatgttgc gggggcttta tccctggtgg cattggttgc tggagagaga aaacccccgc
    19981 acgttgcagg tatgcacctg acaacataac gggggctaat cttgaaccta gaccactcaa
    20041 gaatagccgc gaaacgttgt
//
LOCUS       DAPGOF010000086        20019 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.086, whole genome shotgun sequence.
ACCESSION   DAPGOF010000086 DAPGOF010000000
VERSION     DAPGOF010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20019
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.086"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(226..1038)
                     /locus_tag="RHV13_002699"
     CDS             complement(226..1038)
                     /locus_tag="RHV13_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HDV1242220.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPVDAIVAGFTSIPSQGDNMPA
                     YHASPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAASAEDGWQRMLEWFKQYGGKRS"
     gene            1300..2061
                     /gene="udp"
                     /locus_tag="RHV13_002700"
     CDS             1300..2061
                     /gene="udp"
                     /locus_tag="RHV13_002700"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HDV1242221.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            2202..3629
                     /gene="rmuC"
                     /locus_tag="RHV13_002701"
     CDS             2202..3629
                     /gene="rmuC"
                     /locus_tag="RHV13_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HDV1242222.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            3724..4479
                     /gene="ubiE"
                     /locus_tag="RHV13_002702"
     CDS             3724..4479
                     /gene="ubiE"
                     /locus_tag="RHV13_002702"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HDV1242223.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            4493..5098
                     /gene="ubiJ"
                     /locus_tag="RHV13_002703"
     CDS             4493..5098
                     /gene="ubiJ"
                     /locus_tag="RHV13_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HDV1242224.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKALRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALIKRLEKLEAK"
     gene            5095..6735
                     /gene="ubiB"
                     /locus_tag="RHV13_002704"
     CDS             5095..6735
                     /gene="ubiB"
                     /locus_tag="RHV13_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HDV1242225.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKPLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            6814..7083
                     /gene="tatA"
                     /locus_tag="RHV13_002705"
     CDS             6814..7083
                     /gene="tatA"
                     /locus_tag="RHV13_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HDV1242226.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            7087..7602
                     /gene="tatB"
                     /locus_tag="RHV13_002706"
     CDS             7087..7602
                     /gene="tatB"
                     /locus_tag="RHV13_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HDV1242227.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            7605..8381
                     /gene="tatC"
                     /locus_tag="RHV13_002707"
     CDS             7605..8381
                     /gene="tatC"
                     /locus_tag="RHV13_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HDV1242228.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            8423..9205
                     /gene="tatD"
                     /locus_tag="RHV13_002708"
     CDS             8423..9205
                     /gene="tatD"
                     /locus_tag="RHV13_002708"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HDV1242229.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(9202..9690)
                     /gene="rfaH"
                     /locus_tag="RHV13_002709"
     CDS             complement(9202..9690)
                     /gene="rfaH"
                     /locus_tag="RHV13_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HDV1242230.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            9857..11350
                     /gene="ubiD"
                     /locus_tag="RHV13_002710"
     CDS             9857..11350
                     /gene="ubiD"
                     /locus_tag="RHV13_002710"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HDV1242231.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            11396..12097
                     /gene="fre"
                     /locus_tag="RHV13_002711"
     CDS             11396..12097
                     /gene="fre"
                     /locus_tag="RHV13_002711"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HDV1242232.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(12289..13452)
                     /gene="fadA"
                     /locus_tag="RHV13_002712"
     CDS             complement(12289..13452)
                     /gene="fadA"
                     /locus_tag="RHV13_002712"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HDV1242233.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(13462..15651)
                     /gene="fadB"
                     /locus_tag="RHV13_002713"
     CDS             complement(13462..15651)
                     /gene="fadB"
                     /locus_tag="RHV13_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HDV1242234.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVDDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            15841..17172
                     /gene="pepQ"
                     /locus_tag="RHV13_002714"
     CDS             15841..17172
                     /gene="pepQ"
                     /locus_tag="RHV13_002714"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HDV1242235.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            17172..17786
                     /locus_tag="RHV13_002715"
     CDS             17172..17786
                     /locus_tag="RHV13_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HDV1242236.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            17825..19276
                     /gene="trkH"
                     /locus_tag="RHV13_002716"
     CDS             17825..19276
                     /gene="trkH"
                     /locus_tag="RHV13_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HDV1242237.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            19288..19833
                     /gene="hemG"
                     /locus_tag="RHV13_002717"
     CDS             19288..19833
                     /gene="hemG"
                     /locus_tag="RHV13_002717"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HDV1242238.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFMEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         4638 a         5092 c         5489 g         4800 t
ORIGIN      
        1 tgttgataat aattaatgtt tgtttgtgaa tgggagtgga tggtaaatct ctaaggtgtc
       61 ttccattgaa gagactcatt tatgactatg acttgccgac tttcttaagg tcaaatagcg
      121 gagtcgtaat ctccaatcaa ttcatactga ttattttttg ggggacagag atggcagcaa
      181 aacgcactcg ttcactcacc atctcaaata tagactaaga ggaaactaac tccgcttccc
      241 accatactgc ttaaaccatt ccagcatacg ctgccagcca tcttctgcag atgcggcatg
      301 atagctcggg cgataatcag cgttgaatgc atgcccggca tccgggtaca cgataatctc
      361 tgctttcgca ttagcagccc gcagcgcctg gcgcatggtt tcaacgctct cctgcggaat
      421 gctgttatcc tgaccaccat ataagccgag aaccggcgcg ttaagatcgg ttgcgatatc
      481 aacaggttgt ttcggtgaat tcaacgactt gtcgcccgtc agtttgccgt accacgccac
      541 tgcggctttt agctgtggat tatgcgcggc atacagccag gtgatacgtc caccccagca
      601 gaatccggtg atcattaaac gatgaacatc gccgccgttg cgggaagccc aactggcgac
      661 atgatcgaga tcggccagca cctgcgagtc aggcactttt gctaccagac cgctaagcaa
      721 cgtggggata tcggcaaaat cattcggatc gccttcgcgg aagtaaagtt caggtgcgat
      781 agccagatac ccctccagcg ccagacggcg acaaatgtcg cggatatgtt catgcacgcc
      841 aaaaatttcc tgcactacaa tgaccactgg cagtgggcca tcgctttgct ttggactggc
      901 atggtaagca ggcatgttat ccccttgtga agggatagag gtgaagcccg ccacaatcgc
      961 gtcgaccggg gtctgaacga tggtcgaagc gagaggcgat gcagcaggtg caaatccaga
     1021 ttgttgtgtt gttgccatgg tattctccgt acccttataa aaatgttgcg caatgttaac
     1081 tatagtcagc atgcaacaaa tcacattgcc tgaatcggct catcttttat gcagtcctgc
     1141 agaatgaaga gtgatttatg tgatttgcat cacttttggt gggtaaattt atgcaacgca
     1201 tttgcgtcat ggtgatgagt atcacgaaaa aatgttaaac ccttcggtaa agtgtctttt
     1261 tgcttcttct gactaaaccg attcacagag gagttgtata tgtccaagtc tgatgttttt
     1321 catctcggcc tcactaaaaa cgatttacaa ggggctacgc ttgccatcgt ccctggcgac
     1381 ccggatcgtg tggaaaagat cgccgcgctg atggataagc cggttaagct ggcatctcac
     1441 cgcgaattca ctacctggcg tgcagagctg gatggtaaac ctgttatcgt ctgctctacc
     1501 ggtatcggcg gcccgtctac ctctattgct gttgaagagc tggcacagct gggcattcgc
     1561 accttcctgc gtatcggtac aacgggcgct attcagccgc atattaatgt gggtgatgtc
     1621 ctggttacca cggcgtctgt ccgtctggat ggcgcgagcc tgcacttcgc accgctggaa
     1681 ttcccggctg tcgctgattt cgaatgtacg actgcgctgg ttgaagctgc gaaatccatt
     1741 ggagcgacaa ctcacgttgg cgtgacagct tcttctgata ccttctaccc aggtcaggaa
     1801 cgttacgata cttactctgg tcgcgtagtt cgtcacttta aaggttctat ggaagagtgg
     1861 caggcgatgg gcgtaatgaa ctatgaaatg gaatctgcaa ccctgctgac catgtgtgca
     1921 agtcagggcc tgcgcgccgg tatggtagcg ggtgttatcg ttaaccgcac ccagcaagag
     1981 atcccgaatg ctgagacgat gaaacaaacc gaaagccatg cggtgaaaat cgtggtggaa
     2041 gcggcgcgtc gtctgctgta attctcttct cctgtctgaa ggccgacgcg ttcggccttt
     2101 tgtatttttg cgtagcgcct cgcaggaaat gcctttccaa ctggacgttt gtacagcaca
     2161 attctatttt gtgcgggtaa gttgttgcgt caggaggcgt tgtggatttc tcaatcatgg
     2221 tttacgcagt tattgcgttg gtgggtgtgg caattggctg gctgtttgcc agttatcaac
     2281 atgcgcagca aaaagccgag caattagctg aacgtgaaga gatggtcgcg gagttaagcg
     2341 cggcaaaaca acaaattacc caaagcgagc actggcgtgc agagtgcgag ttactcaata
     2401 acgaagtgcg cagcctgcaa agtattaaca cctctctgga ggccgatctg cgtgaagtaa
     2461 ccacgcggat ggaagccgca cagcaacatg ctgacgataa aattcgccag atgattaaca
     2521 gcgagcagcg cctcagtgag cagtttgaaa acctcgccaa ccgtattttt gagcacagca
     2581 atcgccgggt tgatgagcaa aaccgtcaga gtctgaacag cctgttgtcg ccgctacgtg
     2641 aacaactgga cggtttccgc cgtcaggttc aggacagctt cggtaaagaa gcacaagaac
     2701 gccataccct gacccacgaa attcgcaatc tccagcaact caacgcgcaa atggcccagg
     2761 aagcgatcaa cctgacgcgc gcgctgaaag gcgacaataa aacccagggc aactggggcg
     2821 aggtagtatt gacgcgggtg ctggaggctt ccggtctgcg tgaagggtat gaatatgaaa
     2881 cccaggtcag catcgaaaat gacgcccgct cgcggatgca gccggatgtc atcgtgcggc
     2941 tgccgcaggg aaaagatgtg gtgatcgacg ccaaaatgac gctggtcgcc tatgaacgct
     3001 attttaacgc cgaagacgac tacacccgcg aaagcgcgct acaggaacat atcgcgtcgg
     3061 tgcgtaacca tatccgtttg ctgggacgca aagattatca acagctgccg gggctgcgaa
     3121 ctctggatta cgtgctgatg tttattcccg ttgaacccgc ttttttactg gcgcttgacc
     3181 gccagccgga gctgatcacc gaagcgttga aaaacaatat catgctggtt agcccgacta
     3241 cgctgctggt ggcgctgcgc actatcgcca acctgtggcg ttatgagcat caaagccgca
     3301 acgcccagca aatcgccgat cgtgccagca agctgtacga caagatgcgt ttgttcatcg
     3361 atgacatgtc cgcgattggt caaagtctcg acaaagcgca ggataactat cggcaggcaa
     3421 tgaaaaaact ctcttcaggg cgcggaaatg tgctggcgca ggcagaagcg tttcgcggtt
     3481 taggagtaga aattaaacgc gagattaatc cggatttggc tgaacaggcg gtgagccagg
     3541 atgaagagta tcgacttcgg tcggttccgg agcagccgaa tgatgaagct tatcaacgcg
     3601 atgatgaata taatcagcag tcgcgctagc ccattgggag tagttaagcc gggtagaaat
     3661 ctagggcatc gacgcccaat ctgttacact tctggaacaa ttttttgatg agcaggcatt
     3721 gagatggtgg ataagtcaca agaaacgacg cactttggtt ttcagaccgt cgcgaaggaa
     3781 caaaaagcgg atatggtcgc ccacgttttc cattccgtgg catcaaaata cgatgtcatg
     3841 aatgatttga tgtcatttgg tattcatcgt ttgtggaagc gattcacgat tgattgcagc
     3901 ggcgtacgcc gtgggcagac cgtgctggat ctggctggtg gcaccggcga cctgacagcg
     3961 aaattctccc gcctggtcgg agaaactggc aaagtggtcc ttgctgatat caatgaatcc
     4021 atgctcaaaa tgggccgcga gaagctgcgt aatatcggtg tgattggcaa cgttgagtat
     4081 gttcaagcga acgctgaggc gctgccgttc ccggataaca cctttgattg catcaccatt
     4141 tcgtttggtc tgcgtaacgt caccgacaaa gataaagcac tgcgttcaat gtatcgcgtg
     4201 ctgaaacccg gcggccgcct gctggtgctt gagttctcga agccaattat cgagccgctg
     4261 agcaaagcct atgatgcata ctccttccat gtgctgccgc gtattggctc actggtcgcg
     4321 aacgacgccg acagctaccg ttatctggca gaatccatcc gtatgcatcc cgatcaggat
     4381 accctgaaag ccatgatgca ggatgccgga ttcgaaagtg tcgactacta caatctgacg
     4441 gcaggggttg tggcgctgca tcgtggttat aagttctgac aggagaccgg aaatgccttt
     4501 taaaccttta gtgacggcag gaattgaaag tctgctcaac accttcctgt atcgctcacc
     4561 cgcgctgaaa acagcccgct cgcgtctgtt gggcaaagtg ctgcgcgtgg aggtaaaagg
     4621 cttttcgacg tcattgattc tggtgttcag cgaacgtcag gttgatgtac tgggcgaatg
     4681 ggcaggcgat gctgactgca ccgttatcgc ctacgccagt gtgttgccga aacttcgcga
     4741 tcgccagcag cttgccgccc tgattcgcag tggtgagctg gaagtgcagg gcgatattca
     4801 ggtggtgcaa aacttcgttg cgctggcaga tctggcagag ttcgaccctg cggaactgct
     4861 ggccccttat accggtgata tcgccgctga aggaatcagc aaagccctgc gcggaggcgc
     4921 aaagttcctg catcacggca ttaagcgcca gcaacgttat gtggcggaag ccattactga
     4981 agagtggcgt atggcacccg gtccgcttga agtggcctgg tttgcggaag agacggctgc
     5041 cgtcgagcgt gctgttgatg ccctgatcaa acggctggaa aaactggagg ctaaatgacg
     5101 ccaggtgaag tacggcgcct atatttcatc attcgcactt ttttaagcta cggacttgat
     5161 gaactgatcc ccaaaatgcg tatcaccctg ccgctacggc tatggcgata ctcattattc
     5221 tggatgccaa atcggcataa agacaaacct ttaggtgagc gactacgact ggccctgcaa
     5281 gaactggggc cggtatggat caagttcggg caaatgttat caacccgccg cgatcttttt
     5341 ccgccgcata ttgccgatca gctggcgtta ttgcaggaca aagttgctcc gtttgatggc
     5401 aagctggcga agcagcagat tgaagctgca atgggcggct tgccggtaga agcgtggttt
     5461 gacgattttg aaatcaagcc gctggcttct gcttctatcg cccaggttca taccgcgcga
     5521 ttgaaatcga atggtaaaga ggtggtgatt aaagtcatcc gcccggatat tttgccggtc
     5581 attaaagcag acctgaaact tatctaccgt ctggctcgct gggtgccgcg tttgctgccg
     5641 gatggtcgcc gtctacgccc aaccgaagtg gtgcgcgagt acgaaaagac cttgattgat
     5701 gaactgaatt tgctgcggga atctgccaac gccattcagc ttcggcgaaa ttttgaagac
     5761 agcccgatgc tctacatccc ggaggtttac cctgactatt gtagtgaagg gatgatggtg
     5821 atggagcgca tttacggcat tccggtgtct gatgttgcgg cgctggagaa aaacggcacc
     5881 aacatgaaat tgctggcgga acgcggcgtg caggtgttct tcactcaggt ctttcgcgac
     5941 agctttttcc atgccgatat gcaccctggc aacatcttcg taagctatga acacccggaa
     6001 aacccgaaat atatcggcat tgattgcggg attgttggct cgctaaacaa agaagataaa
     6061 cgctatctgg cagaaaactt tatcgccttc tttaatcgcg actatcgcaa agtggcagag
     6121 ctacacgtcg attctggctg ggtgccacca gataccaacg ttgaagagtt cgaatttgct
     6181 attcgtacgg tctgtgaacc tatctttgag aaaccgctgg ccgaaatttc gtttggacat
     6241 gtactgttaa atctgtttaa tacggcgcgt cgcttcaata tggaagtgca gccgcaactg
     6301 gtgttactcc agaaaaccct gctctacgtc gaaggggtag gacgccagct ttatccgcag
     6361 ctcgatttat ggaaaacagc gaagcctttc ctggagtcgt ggattaaaga tcaggtcggt
     6421 attcctgcgc tggtgagagc atttaaagaa aaagcgccgt tctgggtcga aaaaatgcca
     6481 gaactgcctg aactggttta cgacagtttg cgccagggca agtatttaca gcacagtgtt
     6541 gataagattg cccgcgagct tcagtcaaat catgtacgtc agggacaatc gcgttatttt
     6601 ctcggaattg gcgctacgtt agtattaagt ggcacattct tgttggtcag ccgacctgag
     6661 tgggggctga tgcccggctg gttaatggca ggtggtctga tcgcctggtt tgtcggttgg
     6721 cgcaaaacac gctgattttt tcatcgctca aggcgggccg tgtaacgtat aatgcggctt
     6781 tgtttaatca tcatctacca cagaggaaca tgtatgggtg gtatcagtat ttggcagtta
     6841 ttgattattg ccgtcatcgt tgtactgctt tttggcacca aaaagctcgg ctccatcggt
     6901 tccgatcttg gtgcgtcgat caaaggcttt aaaaaagcaa tgagcgatga tgaaccaaag
     6961 caggataaaa ccagtcagga tgctgatttt actgcgaaaa ctatcgccga taagcaggcg
     7021 gatacgaatc aggaacaggc taaaacagaa gacgcgaagc gccacgataa agagcaggtg
     7081 taatccgtgt ttgatatcgg ttttagcgaa ctgctattgg tgttcatcat cggcctcgtc
     7141 gttctggggc cgcaacgact gcctgtggcg gtaaaaacgg tagcgggctg gattcgcgcg
     7201 ttgcgttcac tggcgacaac ggtgcagaac gaactgaccc aggagttaaa actccaggag
     7261 tttcaggaca gtctgaaaaa ggttgaaaag gcgagcctca ctaacctgac gcccgaactg
     7321 aaagcgtcga tggatgaact acgccaggcc gcggagtcga tgaagcgttc ctacgttgca
     7381 aacgatcctg aaaaggcgag cgatgaagcg cacaccatcc ataacccggt ggtgaaagat
     7441 aatgaagctg cgcatgaggg cgtaacgcct gccgctgcac aaacgcaggc cagttcgccg
     7501 gaacagaagc cagaaaccac gccagagccg gtggtaaaac ctgctgcgga cgctgaaccg
     7561 aaaaccgctg caccttcccc ttcgtcgagt gataaaccgt aaacatgtct gtagaagata
     7621 ctcaaccgct tatcacgcat ctgattgagc tgcgtaagcg tctgctgaac tgcattatcg
     7681 cggtgatcgt gatattcctg tgtctggtct atttcgccaa tgacatctat cacctggtat
     7741 ccgcgccatt gatcaagcag ttgccgcaag gttcaacgat gatcgccacc gacgtggcct
     7801 cgccgttctt tacgccgatc aagctgacct ttatggtgtc gctgattctg tcagcgccgg
     7861 tgattctcta tcaggtgtgg gcatttatcg ccccagcgct gtataagcat gaacgtcgcc
     7921 tggtggtgcc gctgctggtt tccagctctc tgctgtttta tatcggcatg gcgttcgcct
     7981 actttgtggt ctttccgctg gcatttggct tccttgccaa taccgcgccg gaaggggtgc
     8041 aggtatccac cgacatcgcc agctatttaa gcttcgttat ggcgctgttt atggcgtttg
     8101 gtgtctcctt tgaagtgccg gtggcaattg tgctgctgtg ctggatgggg attacctcgc
     8161 cagaagactt acgcaaaaaa cgcccgtatg tgctggttgg tgcattcgtt gtcgggatgt
     8221 tgctgacgcc gccggatgtc ttctcgcaaa cgctgttggc gatcccgatg tactgtctgt
     8281 ttgaaatcgg tgtcttcttc tcacgctttt acgttggtaa agggcgaaat cgggaagagg
     8341 aaaacgacgc tgaagcagaa agcgaaaaaa ctgaagaata aattcaaccg cccgtcaggg
     8401 cggttgtcat atggagtaca ggatgtttga tatcggcgtt aatttgacca gttcgcaatt
     8461 tgcgaaagac cgtgatgatg ttgtagcgcg cgcttttgac gcgggagtta atgggctact
     8521 catcaccggc actaacctgc gtgaaagcca gcaggcgcaa aagctggcgc gtcagtattc
     8581 gtcctgttgg tcaacggcgg gcgtacatcc tcacgacagc agccagtggc aagctgcgac
     8641 tgaagaagcg attattgagc tggccgcgca gccagaagtg gtggcgattg gtgaatgtgg
     8701 tctcgacttt aaccgcaact tttcgacgcc ggaagagcag gaacgcgctt ttgttgccca
     8761 gctacgcatt gccgcagatt taaacatgcc ggtatttatg cactgtcgcg atgcccacga
     8821 gcggtttatg acattgctgg agccgtggct ggataaactg cctggtgcgg ttcttcattg
     8881 ctttaccggc acacgcgaag agatgcaggc gtgcgtggcg catggaattt atatcggcat
     8941 taccggttgg gtttgcgatg aacgacgcgg actggagctg cgggaacttt tgccgttgat
     9001 tccggcggaa aaattactga tcgaaactga tgcgccgtat ctgctccctc gtgatctcac
     9061 gccaaagcca tcatcccggc gcaacgaacc agcccatctg ccccatattt tgcaacgtat
     9121 tgcgcactgg cgtggagaag atgccgcatg gctggctgcc accacggatg ctaatgtcaa
     9181 aacactgttt gggattgcgt tttagagttt gcggaactcg gtgttcttca cactgtgctt
     9241 aatctcttta ttaataagat taagcaatag catggagcga gcctcaccat cgggttctgt
     9301 gaaaatggcc tgaaagcctt cgaacgcgcc ttcggtaata atcaccttat cacccggata
     9361 aggggttgcc ggatcgacaa tgtctttcgg tttatatacc gatagctgat gaatcaccgc
     9421 cgatgggact atcgctggcg acgcgccaaa gcgcacgaag tggctgacac cgcgggtcgc
     9481 gttgatagtc gtggtatgaa tcacttctgg atcaaattcc acaaacaggt agttggggaa
     9541 caatggctca ctgactgcag tacgtttgcc acgcacgatt ttttccaggg tgatcatcgg
     9601 tgccaggcaa ttcacagcct gtctttcgag gtgttcctgg gcacgttgaa gttgcccgcg
     9661 cttgcagtac agtaaatacc aggattgcat aatgactctt atccgtttaa tcggggcgca
     9721 aggatagcaa aagctttacg ctaagttaat tatattcccc ggtttgcgtt ataccgtcag
     9781 agttcacgct aatttaacaa atttacagca tcgcaaagat gaacgccgta taatgggcgc
     9841 agattaagag gctacaatgg acgccatgaa atataacgat ttacgcgact tcttgacgct
     9901 gcttgaacag cagggtgagc taaaacgtat cacgctcccg gtggatccgc atctggaaat
     9961 cactgaaatt gctgaccgca ctttgcgtgc cggtgggcct gcgctgttgt tcgaaaaccc
    10021 taaaggctac tcaatgccgg tgctgtgcaa cctgttcggt acgccaaagc gcgtggcgat
    10081 gggcatgggg caggaagatg tttcggcgct gcgtgaagtt ggtaaattat tggcgtttct
    10141 gaaagagccg gagccgccaa aaggtttccg cgacctgttt gataaactgc cgcagtttaa
    10201 gcaagtattg aacatgccga caaagcggct gcgcggtgcg ccttgccaac aaaaaatcgt
    10261 ctctggcgat gacgtcgatc tcaatcgcat tcccattatg acctgctggc cggaagatgc
    10321 cgcgccgctg attacctggg ggctgaccgt aacgcgcggc ccgcataaag agcggcagaa
    10381 tctgggcatt tatcgccagc agctgattgg taaaaacaaa ctgattatgc gctggctgtc
    10441 gcatcgcggc ggcgcgctgg attatcagga gtggtgtgcg gcgcatccgg gcgaacgttt
    10501 cccggtttct gtggcgctgg gtgccgatcc cgccacgatt ctcggtgcag tcacccccgt
    10561 tccggatacg ctttcagagt atgcgtttgc cggattgcta cgcggcacca aaaccgaagt
    10621 agtaaagtgt atctccaatg accttgaagt gcccgccagt gcggagattg tgctggaagg
    10681 gtatatcgaa caaggcgaaa ctgcgccgga agggccgtat ggcgaccaca ccggttacta
    10741 taatgaagtc gatagtttcc cggtatttac cgtgacgcat attacccagc gtgaagatgc
    10801 gatttaccat tccacctata ccgggcgtcc gccagatgag cccgcggtgc tgggtgtcgc
    10861 actgaacgaa gtgtttgtgc cgattctgca aaaacagttc ccggaaattg tcgattttta
    10921 cctgccgccg gaaggctgct cttatcgcct ggcggtagtg acaatcaaaa aacagtacgc
    10981 cggacacgcg aagcgcgtca tgatgggcgt ctggtcgttc ttacgccagt ttatgtacac
    11041 taaatttgtg atcgtttgcg atgatgacgt taacgcacgc gactggaacg atgtgatttg
    11101 ggcgattacc acccgtatgg acccggcgcg ggatactgtt ctggtagaaa atacgcctat
    11161 tgattatctg gattttgcct cgcctgtctc cgggctgggt tcaaaaatgg ggctggatgc
    11221 cacgaataaa tggccggggg aaacccagcg tgaatgggga cgtcccatca aaaaagatcc
    11281 agatgttgtc gcgcatattg acgccatctg ggatgaactg gctattttta acaacggtaa
    11341 aagcgcctga tgcgcgtttg ttttgcccta tttatcgatc cgacagagaa agcgcatgac
    11401 aaccttaagc tgtaaagtga cctcggtaga agctatcacg gataccgtat atcgtgtccg
    11461 catcgtgcca gacgcggcct tttcttttcg cgctggtcag tatttgatgg tagtgatgga
    11521 tgagcgcgac aaacgtccgt tctcaatggc ctcgacgccg gatgaaaaag ggtttatcga
    11581 gctgcatatt ggcgcttctg aaatcaacct ttacgcgaaa gcagtcatgg accgcatcct
    11641 caaagatcat caaatcgtgg tcgatattcc ccacggagaa gcgtggctgc gcgatgatga
    11701 agagcgtccg atgattttga ttgcgggcgg caccgggttc tcttatgccc gctcgatttt
    11761 gctgacagcg ttggcgcgta acccaaaccg tgatatcacc atttactggg gcgggcgtga
    11821 agagcagcat ctgtatgatc tctgcgagct tgaagcgctt tcgttaaagc accctggtct
    11881 gcaagtggtg ccggtggttg aacaaccgga agcgggctgg cgtgggcgta ctggcaccgt
    11941 gttaacggcg gtattgcagg atcacggtac gctggcagag catgatatct atattgccgg
    12001 acgttttgag atggcgaaaa tagcccgtga cctgttttgc agtgagcgta atgcgcggga
    12061 agatcgcctg tttggcgatg cgtttgcatt tatctgagat ataaaaaaac ccgcccctga
    12121 caggcgggaa gaacggcaac taaaacttta ttcagtgaca tttagatcta tgtcgcatcc
    12181 ggcattcggc acacgatgcc tgatgcgacg ctggcgcgtc ttatcaggcc tacattggtg
    12241 ccggatcggt agaccggata aggcgttcac gccgcatccg gcaagtggtt aaacccgctc
    12301 aaacaccgtc gcaataccct gacccagacc gatacacatc gtcgccagac caaactgaac
    12361 gtctttgtgt tccatcagat tcagcagcgt ggtgctgata cgcgcaccgg aacagcccag
    12421 cggatgaccc agcgcgatcg cgccaccatt gaggttgatc ttctcgtcaa tctgctccat
    12481 tagcccgaga tctttaatac atggcaggat ctgcgcggca aaggcttcat tcatttcaaa
    12541 tacgccgata tcgctggcag aaagccccgc ttttttcagc gccagcttcg aggccggaac
    12601 cgggccgtaa cccataatcg acggatcaca accaacgacc gccatcgaac gcacgcgggc
    12661 gcgcggctta agacctaatt cacgggcgcg gctttcactc atcaccagca tggcggcggc
    12721 accatcggaa agcgcagaag atgtacccgc cgttaccgta ccgctgacgg gatcaaacgc
    12781 cggacgcagc gtggcgaggg cttccacggt ggtttccggg cgaatcactt cgtcgtaatt
    12841 aaactgcttc aggacgccgt cggcatcgtg accaccagtt gggatgattt catttttaaa
    12901 tgcgcccgac tgcgtggcgg cccaggcgcg ggcgtgtgac cgcgcggcaa aggcatcctg
    12961 catttcacgg ctgataccgt gcatacgcgc cagcatttct gccgttaagc ccatcatgcc
    13021 cgccgctttg gcgacattgc ggctcaggcc agggtgaaaa tcgacgccgt gactcatcgg
    13081 cacatggccc atatgctcca cgccgccaac cagacatgcc tgcgcatcgc cggtcatgat
    13141 cattcgtgct gcgtcatgca gtgcctgcat ggatgaacca cacaagcgat tgacagtaac
    13201 cgccgggaca gagtgcggca cttctgccag cagcgccgcg ttacgggcga tattaaaacc
    13261 ctgctccagc gtctgctgca cacaacccca gtaaatatcg tcgagggccg ccgcttccag
    13321 cgccgggtta cgcgccagca ggctacgcat taaatgagcg gagagatctt ctgcacgcac
    13381 gttacgaaaa gcaccgccct tcgaacggcc catcggggtg cgaattgcat cgacaatgac
    13441 aacctgttcc attgtgactc cttaagccgt tttcaggtcg ccaaccggac gggctggctc
    13501 aaccggggga tagtacggtt cgttatgacg cgctttatta cgcagacctt ccggcacttc
    13561 gtacagcggg ccgaggtgct gatattgctg tgccatatcg aggtattttg cgctgccgag
    13621 ggtgtccagc cagcggaacg cgccgccgtg gaacggaggg aagcccaggc cgtagaccag
    13681 cgccatatcc gcttctgccg gagtggcgat aatgccttct tccagacagc gcaccacttc
    13741 gttgaccatc gggatcatca tgcgggcgat aatctcttct tcgctgaaat cgcgcttcgg
    13801 ctggctgact tctgccagca ggtcgtcaac agcggcgtct tcttctttct tcggcttacc
    13861 tttgctgtct tctttataac gccagaaacc gaggccgttc ttctgaccaa agcggttggc
    13921 atcaaacagc gcgtcgatgg catcgcgata atctttctgc atccgctgcg ggaagcccgc
    13981 tgccatgact gcctgagcgt gatgcgcggt gtcaatgccc accacatcca gcaggtatgc
    14041 cggacccatc ggccagccaa actgtttttc catcactttg tcgatcttgc ggaaatccgc
    14101 gccgtcgcgc agcaactggc tgaaaccggc gaaatacggg aacagcacgc ggttgacgaa
    14161 gaagccgggg cagtcgttaa ccacaattgg cgttttgccc atcttgctcg cccaggcgac
    14221 gactttcgcg atggtttcgt cagagctttt ctcgccgcga ataatttcta ccaacggcat
    14281 ccgatgtacc gggttaaaga agtgcatccc gcagaagttt tccggacgtt ccagcgcgtt
    14341 ggccagttcg ctgataggaa tggttgaagt gttagacgcc agcacggtat ccgggcgtac
    14401 tttttgttcg gtttctgcca gtacggcttt tttcactttc gggttttcga caaccgcttc
    14461 taccacaaca tccacgcggt caaacccggc gtagtcgagc gttgggtgga ttgtagagat
    14521 cacgccagcc agtttcagac catcgatctt gccgcgctca agctgcttgt tcagcagttt
    14581 cgcggcttcg gtcatgccga gggttaacga cttgtcgttg atatctttca tgacaaccgg
    14641 cacgcctttc cacgcagact ggtaagcgat gccgccgccc ataatgcctg cgcccagcac
    14701 agcggcctgt tttggggttt caacgtcttt ggtgagtttc ttcgctttgc cttttacata
    14761 ttgatcgtta aggaatatgc cgaccagtgc gcgagcttcg ttggtatgcg ccagcgggac
    14821 aaaacttttg ttttccaggt ttagggcttc ttcacgacca aaacgggccg cagcttcaat
    14881 ggtttttact gcggtgatgg gggccggata atgtttcccc gccgtttgtg cgaccatccc
    14941 tttagcgatg gtgaagctca tggtagcttc aatcttgctc agttttagcg gttccagctt
    15001 cggctgacgt ttcgctttcc agtcgaggtc gccgttaatg gcctggcgta aaatcgccat
    15061 cgccccttca atcagttttt ctgctttgac tacgccatcc accagaccga ttttcagcgc
    15121 ctgatccgcg ccgacatctt taccggcggc aatgatttcc agcgcactgt cagcgcccag
    15181 catacgtggc atacgtacag aaccgccaaa gcccggcatg atgcccagtt tggtttccgg
    15241 cagaccgatg cgcagatccg gcgtcgccag acgataatcg gtcgccagca cgcattcgca
    15301 gccaccgccc agcgcatagc cattgacggc agcaatggtc ggcaccggca gatcttccag
    15361 gcgattaaac acgctattgg caaagtgcag ccactgactt aactgttctt caggaacgag
    15421 gaacagggac aaaaattcgg tgatatcagc accgacgata aaggctgctt tgttcgaacg
    15481 cagcagcagc ccttttagat ctgattgctg ttccagcacg ccgatggcct cgccgaggct
    15541 ggcgacggtc gcagtgtcga gtttattaac tgaacctggg gcatcaaata ccagttcggc
    15601 aatgccatct tccagccagt caaggtacag ggtgtcgcct ttgtaaagca tgtcagtctc
    15661 ctgaatccgc aaggtgatct ggtcgtacca gatgagtcga agtgtgtatt ttgtgttaaa
    15721 aatatgcaaa taaaagatta aagaaatgcc gatctgatca cgctcggcag aaatcacgct
    15781 ctggatgaac gatgtgctaa gatgcggaga cttaaggtca aaaaaacaga agggtaaaaa
    15841 atggaatcac tggcctcgct ctataaaaat catatagcta ccttacaaga acggactcgc
    15901 gatgcgctgg cgcgcttcaa gctggatgcg ttacttattc actccggcga gctgttcaat
    15961 gtttttctcg acgatcatcc ctatccgttt aaagtgaacc cgcaattcaa agcgtgggtg
    16021 ccggtaactc aggtgccaaa ctgctggttg ctggtggatg gcgtgaacaa gccgaaactg
    16081 tggttttatc tgccggttga ttactggcac aacgtcgaac cgctgccgac ctccttctgg
    16141 actgaagatg tagaagtgat cgcgctgccg aaagccgatg gaattggtag tctgttgcct
    16201 gctgcgcgcg gcaatatcgg ttatatcggt ccggtgccgg aacgtgcgct gcaactgggt
    16261 attgaggcca gcaatatcaa cccgaaaggg gttatcgact acctgcatta ctaccgctcc
    16321 ttcaaaaccg agtacgagct ggcttgtatg cgtgaagcgc agaaaatggc ggtcaacggt
    16381 catcgcgcgg cagaagaagc gttccgttct ggcatgagcg agttcgatat caatattgcc
    16441 tatctgactg cgaccggtca tcgtgatacc gacgtacctt acagcaacat tgtggcgctc
    16501 aacgaacacg ctgcggtgct gcattacacc aaactggatc atcaggcgcc ggaagagatg
    16561 cgcagcttcc tgctggatgc cggggccgaa tataacggct atgccgctga cctgacccgt
    16621 acctggtcgg caaaaagtga caacgattac gcacagctgg tgaaagacgt aaatgatgaa
    16681 caactggcgc tgatcgcgac catgaaagct ggcgttagct atgtggatta ccacatccag
    16741 ttccatcagc gcatcgccaa attgctgcgt aaacatcaaa tcatcaccga tatgagtgaa
    16801 gaagcgatgg tcgaaaacga tctcaccgga ccgtttatgc cgcacggtat cggccatccg
    16861 ctgggcctgc aggtgcatga cgtcgccggt tttatgcagg atgatagcgg tacgcacctc
    16921 gcggcaccgg caaaatatcc gtacctgcgc tgcacccgta ttctccagcc gggcatggtg
    16981 ttaaccatcg aaccgggtat ctacttcatt gaatcgctac tggcaccgtg gcgtgaaggg
    17041 cagttcagca agcacttcaa ctggcagaaa attgaagcac tgaaaccgtt cggcggcatt
    17101 cgtatcgaag acaacgtggt gatccacgaa aacaacgtgg aaaacatgac ccgggatctg
    17161 aaactggcgt gatggaaagc tggttaattc ctgcggcacc ggtcacggtc gttgaagaga
    17221 tcaaaaaaag ccgcttcata acgatgttgg cgcataccga tggcgttgag gcggcgaaag
    17281 cgtttgttga atcggtgcgg gcagaacacc ccgatgcccg ccaccattgc gtggcgtggg
    17341 tggcgggtgc gccggatgat tctcaacagc tgggtttctc tgacgacggg gagccggcgg
    17401 gaacggcagg taaaccgatg ctcgcccagc taatgggcag cggcgtcggg gaaattaccg
    17461 ctgtggtagt gcgctactac ggcggcatat tgcttggcac cggtgggtta gtgaaagcgt
    17521 atggcggcgg cgtgaatcag gcgctgcggc agctaacgac ccaacgcaag acgccattaa
    17581 ccgaatatac tttgcaatgt gaatatagtc agttaaccgg cattgaagcg ttgctggggc
    17641 agtgtgacgg caaaattatc aacagtgatt atcaggcatt cgttctgctg cgggtggcgc
    17701 ttccggcggc gaaagtggct gaattttccg caaagctggc ggattttagc cgtggttcat
    17761 tgcaattgtt agcgattgaa gaataatccc cacttcgttt tgcagaacta aggaagcggc
    17821 agagatgcat tttcgcgcca ttacccgaat cgttggacta ctggtcatct tattttcagg
    17881 gaccatgatt atccctgggc tggtagcact catctaccgg gatggagcgg gccgcgcttt
    17941 tacccagacc ttttttgtcg ccctcgccat tggctctatg ctgtggtggc cgaaccgcaa
    18001 agagaagggc gaactgaaat cccgtgaggg gtttctgata gtggtgctgt tctggaccgt
    18061 gctgggtagt gtcggtgcgc tcccttttat cttctcggaa agcccgaacc tcacgattac
    18121 cgatgcgttt tttgaatctt tctctggcct gaccaccacc ggggccacta cgctggtggg
    18181 gctggattcg ctccctcatg ccatcctctt ttatcgccag atgctgcaat ggtttggcgg
    18241 gatggggatc atcgtgttag cggttgcgat actgcctatc ctcggcgtgg gtgggatgca
    18301 gctctatcgc gcagaaatgc ccggcccgct gaaagataac aaaatgcgcc cgcgaattgc
    18361 ggaaacggcg aaaaccctgt ggttgattta tgtcttgctg accgtcgcct gtgcgctggc
    18421 gttgtggttt gccggaatgg atgcctttga tgccatcggc catagctttg cgactatcgc
    18481 tattggcggc ttctcgacac atgatgccag tatcggttat ttcgacagcc cgactattaa
    18541 cactatcatt gctatcttcc tgctgatctc cggctgtaac tacggtctgc acttttcact
    18601 gttaagtggg cgtagtctga aggtttattg gcgcgatccg gaatttcgca tgtttatcgg
    18661 cgtacagttt acgctggtgg ttatttgtac cctcgtgctg tggtttcata atgtctatag
    18721 ttcggcgctg atgacaatta accaggcgtt tttccaggtg gtgtcgatgg cgacaaccgc
    18781 cgggtttacg acggacagca ttgcccgctg gccgctcttt ttgccggtac tgctgttatg
    18841 ttcagctttt atcggcggtt gcgccgggtc aacgggcggt ggcctgaaag tgattcgcat
    18901 tctgctgctg ttcaagcagg ggaaccgtga gctaaaacga ctggtccacc cgaacgccgt
    18961 ctatagtatt aagctgggta accgtgcact accggaacgc atcctcgaag cggtgtgggg
    19021 atttttctcc gcctatgctc tggtgtttat cgtcagtatg ctggcgatta tcgccaccgg
    19081 cgtggatgac ttttctgcct ttgcctcggt tgttgcgaca ttgaataacc tggggccggg
    19141 gcttggcgtg gttgctgata actttaccag catgaacccg gtggctaaat ggatccttat
    19201 tgccaacatg ctgtttggtc gtctcgaggt ctttacattg ctggtgcttt ttaccccgac
    19261 tttctggcgt gaatgatgga gtaatacgtg aaaacattaa ttcttttctc aacaagggac
    19321 ggacaaacgc gcgagattgc ctcctacctg gcttcggaac tgaaagaact ggggatccag
    19381 gcggatgtcg ccaatgtgca ccgcattgaa gaaccacagt gggaaaacta cgatcgcgtg
    19441 gtgattggcg cttctattcg ctacggtcac taccattctg cgttcatgga atttgttaaa
    19501 aagcatgcga cgcgcttgaa ctccatgccg agcgcctttt actccgtaaa cctggtggcg
    19561 cgtaaaccgg agaagcgtac tccacagacc aacagctacg cgcggaagtt tctgatgaac
    19621 tcgcaatggc gtcccgatcg ctgcgcggtc attgccgggg cgctgcgtta cccacgttat
    19681 cgctggtacg accgttttat gatcaagctg attatgaaga tgtcaggcgg tgaaacggat
    19741 acgcgcaaag aagttgtcta taccgattgg gagcaggtgg cgaatttcgc ccgagaaatc
    19801 gcccatttaa ccgacaaacc gacgctgaaa taagcataaa gaataaaaaa tgcgcggtca
    19861 gaaaattatt ttaaatttcc tcttgtcagg ccggaataac tccctataat gcgccaccac
    19921 tgacacggaa caacggcaaa cacgccgccg ggtcagcggg gttctcctga gaattccggc
    19981 agagaaagca aaaataaatg cttgactctg tagcgggaa
//
LOCUS       DAPGOF010000087        19665 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.087, whole genome shotgun sequence.
ACCESSION   DAPGOF010000087 DAPGOF010000000
VERSION     DAPGOF010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19665
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.087"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            234..734
                     /gene="tssB"
                     /locus_tag="RHV13_002718"
     CDS             234..734
                     /gene="tssB"
                     /locus_tag="RHV13_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HDV1242239.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GQVSNK"
     gene            769..993
                     /locus_tag="RHV13_002719"
     CDS             769..993
                     /locus_tag="RHV13_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242240.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1044..2519
                     /gene="tssC"
                     /locus_tag="RHV13_002720"
     CDS             1044..2519
                     /gene="tssC"
                     /locus_tag="RHV13_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HDV1242241.1"
                     /translation="MSLQEEELVSSHAGQPEQASSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPYSPTDNPVKNFNYYEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAK
                     VEVMDVEGEPGWYQVALSVRPHFKFMGANFELSLVGRLDRE"
     gene            2526..2939
                     /gene="tssE"
                     /locus_tag="RHV13_002721"
     CDS             2526..2939
                     /gene="tssE"
                     /locus_tag="RHV13_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HDV1242242.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2943..4793
                     /gene="tssF"
                     /locus_tag="RHV13_002722"
     CDS             2943..4793
                     /gene="tssF"
                     /locus_tag="RHV13_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HDV1242243.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFNLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            4757..5839
                     /gene="tssG"
                     /locus_tag="RHV13_002723"
     CDS             4757..5839
                     /gene="tssG"
                     /locus_tag="RHV13_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HDV1242244.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTQVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5864..7144
                     /gene="tagH"
                     /locus_tag="RHV13_002724"
     CDS             5864..7144
                     /gene="tagH"
                     /locus_tag="RHV13_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HDV1242245.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHLAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7141..7665
                     /gene="tssJ"
                     /locus_tag="RHV13_002725"
     CDS             7141..7665
                     /gene="tssJ"
                     /locus_tag="RHV13_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HDV1242246.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLQAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPIKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7668..8999
                     /gene="tssK"
                     /locus_tag="RHV13_002726"
     CDS             7668..8999
                     /gene="tssK"
                     /locus_tag="RHV13_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HDV1242247.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQNFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHRARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADVH
                     DSELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQTWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            9004..9765
                     /gene="icmH"
                     /locus_tag="RHV13_002727"
     CDS             9004..9765
                     /gene="icmH"
                     /locus_tag="RHV13_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HDV1242248.1"
                     /translation="MDEGSLSLPPFTGYDEKSLRDYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDVLEFLWLCFSLGFR
                     GRYKVAVQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9774..12545
                     /gene="tssH"
                     /locus_tag="RHV13_002728"
     CDS             9774..12545
                     /gene="tssH"
                     /locus_tag="RHV13_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HDV1242249.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTLTDGSDTLLARYAKNMTADARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYERSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESESESEQDNTGAVPADEADREQPEETAETVSPVQRLAQLTAE
                     LDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLPKWLGDTIKGQD
                     LAIASLHKYLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQYLTTI
                     NMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDVLNLF
                     YQAFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEVLYPVLADFFK
                     PALLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVAIEPEVTDEIMSRVT
                     RAENGARMLESVIDGDMLPPLSLQLLQKMAANTAIARIRLSAVDGAFTADVEDAQNDE
                     SVTKDETVL"
     gene            12542..13285
                     /gene="tagO"
                     /locus_tag="RHV13_002729"
     CDS             12542..13285
                     /gene="tagO"
                     /locus_tag="RHV13_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagO"
                     /protein_id="HDV1242250.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMASVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADSRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            13290..14702
                     /gene="tssA"
                     /locus_tag="RHV13_002730"
     CDS             13290..14702
                     /gene="tssA"
                     /locus_tag="RHV13_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HDV1242251.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWSVAAPQNMAHKKRFASQVIKRFETGIEGFSQNAATAQRDALSGELAKLAQCWQSHN
                     APELALATDDLFALYQRAFNRAAPAPAPTPAASDSSPQTTATSESGVTQPSAPTPQIA
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQSITSTPQAESDGRTPLAA
                     VSADMVADYQSRLASADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADTIR
                     DEVIRFLERLPQLTGLLFNDRTPFLSEQTKQWLAASPDGKVAPVAQIGEESQAARACF
                     AGQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLIQLAQQQYRMLLMIGSQMM
                     VSDWEPSLLTQLEQKFTAEQ"
     gene            14811..18245
                     /gene="tssM"
                     /locus_tag="RHV13_002731"
     CDS             14811..18245
                     /gene="tssM"
                     /locus_tag="RHV13_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HDV1242252.1"
                     /translation="MLILAWIFLLVWIWWKGSTWTLYEEQWLKPLANRWLATAAWGII
                     ALVWLTVRVMKRLQQLEKMQKQQREEAIDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLGEGFPSDIIYAPEGARGTEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRRREHLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRAWLMRSRRRLTVFSTTGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGEDDYGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNE
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFLTRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQRQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KERDEALAEPDYQLLTRLGHEFAPENSTLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLADQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNSVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            18256..19605
                     /locus_tag="RHV13_002732"
     CDS             18256..19605
                     /locus_tag="RHV13_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001087752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HDV1242253.1"
                     /translation="MNSNVLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAESDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQNTRKRLEPQLKSNTENAAQTTVRTLIYAPETQPSSTPEAVVPPLPGLPEMK
                     VEVRSLTENPPQASVIKQGSTVRGFIAGIACSVAVASALWWWQVYPVQQQLLQVNDTA
                     QGAATVWMASPELENYERRLQQLLDTSPVQPLETGMQMMRVADSRWPESLQQQQASTQ
                     WNEALKTRAQNSPQLRGWLQTRQDLHAFADLVMQREKEGLTLSYIKNVIWQAERGLGQ
                     ETPVESLLTQYQDARAQKQNTDALEKQINERLEGVLSRWLLLNNNTIPTIKKGIEFQ"
BASE COUNT         4577 a         5429 c         5487 g         4172 t
ORIGIN      
        1 acacctgacg cgaattacac aggttcacac aatctattta acgcttgcag aatacccctc
       61 tttacgatcg ataaaaagtc cttttcttat ttattttgaa gcgtaaaatg cggggtgatg
      121 aaggagtggt gattttattt tcaccgccat tagggcggca ttgccgatat ttttttgcgg
      181 tacaaatatg ccgtgttcat ggcctgtacg agataacatg acggatcact tttatgagca
      241 aaaaatttga aggcagcgtc gcgcctcgcg agcgtattaa tatatcgtat gtcccaaaaa
      301 cagatggcca gacggctgaa gtggaacttc cgcttaacat gctcgttgtc ggcgataccg
      361 gaaatacgca ggaaacatcc ccactggatg aacgtcaggc agtttccgtt aacaagcaca
      421 atttcggtgc agtgatggct gaagcagcca ttggcctcaa ttttaccgtc cctgccacac
      481 tgaagggaag cacaacggac gatgagctga atgtggcgct gaatatcaag tcactggatg
      541 atttttcacc agatagcgtg gcccggcagg ttccggaagt gaataaattg ctggaactgc
      601 gtgaagcact gactgcactg aaggggccga tgggtaacct tccggcattc cgtacccagc
      661 tccaggcatt gctggaaaat gaagagtccc gcgagcagtt gctcaaagaa atcggccagg
      721 tgagcaataa ataaccacca ctatttcatt aaacagggtt atccaggcat gacggcacac
      781 aaaaatatct ccgggacata ccacctctct gttgcagaca ttctgcaggt ggtttaccag
      841 gtatgtttca gcccgtccgt tgaaatcaac caggatggtg tggctgcgct gattacaaca
      901 ctggatcgcc gtatcagtga tctattggat gagattatcc atttttgtga gtttcagcag
      961 agcgcgtcgc actggcagcg cgtacttcac tgacggcgaa aagccggttt cagaaacaca
     1021 gctaaaacag aaggattttc agtatgtctt tacaggaaga ggaactcgtt tccagtcatg
     1081 ccgggcagcc tgagcaggca tcgtcgctgc ttgatcaaat catggcacag acccgtattc
     1141 agcccggttc ggaaggttat gacgttgccc gccagggtgt gacagccttt attgccagca
     1201 tcctgcaatc cactgcatct gccgaaccgg tcaataaact ggccgtggac agcatgattg
     1261 cagatattga tgaacgcatc agccgccaga tggatgtcat catccatgcg cctgcctttc
     1321 agcaggtcga gtcgttctgg cgctcactga aaaccatggt ggatcgcgtt gatttccgtg
     1381 aaaacatcaa ggtcaacgtg cttcatgtca ccaagcagga acttctggaa gactttgagt
     1441 ttgcgccaga aattattcag tccggatttt ataagcacgt ttattcgtcc ggtttcggac
     1501 agtttggcgg tgaacccatc gctgccgtgc tgggcgctta tgagttcaaa aataccgcgc
     1561 ctgacatgaa actgctgcaa tacgtcagtg ccgtgggcgc aatggcgcac gcaccgttcc
     1621 tgtcctccgt ttccccggag tttatggggc tgaactcgtg gaccgaactg cctaatatca
     1681 aagatcttta tgccatcttt gaaggtccgg cctacaccaa atggcgcgcc ctgcgtgact
     1741 cggaagattc gcgctattta gggcttacgg caccccgctt cctgcttcgc cagccctatt
     1801 caccaacaga taaccctgtt aagaacttta actattacga agatgtcagc cagaaccacg
     1861 aagattatct gtggggtaat acggcctgga tgctggcatg caatatcgca gacagttttg
     1921 ccaaataccg ctggtgtcca aacattatcg gtccgcaaag cggcggcgca gtgaaagatc
     1981 tgccggtgca tctgttcgaa acgatggggc agattcaggc caaaatccca accgaggtgc
     2041 tggtcaccga tcgccgcgaa tttgaactgg ctgaagaggg ttttatcacc cttaccatgc
     2101 gtaaagactc tgataatgca gcctttttct ctgcaaactc ggtacaaaaa ccgaagcact
     2161 ttccgggaaa agatgcagaa accaactata agctgggtac gcagcttcct tatctcttca
     2221 tcatcaatcg gttagcgcac tacatcaagg tgttgcagcg tgaacaactg gggtcatgga
     2281 aagaaaggag cgacttagaa agagagctta atacctggat ccgtcagtat gttgccgacc
     2341 aggaaaaccc gcctgcagac gtgcgcagcc gcaaacctct gcgcgctgcc aaagttgagg
     2401 ttatggatgt agaaggcgaa cccggctggt accaggttgc gctaagcgtg agacctcatt
     2461 ttaaatttat gggggcaaat tttgagcttt ccctggttgg ccggttagac agggagtaac
     2521 acaccatgat accggatcgg gaaagaacca caggttctct ttttgagcgt atggaagcct
     2581 cttcggccag aaaccggcag ggaggcagta ttcattcgct caggcaatcc attcgccaga
     2641 acctacgaaa cattcttaat acccggtccg gtagttgccg gggtgcgcct gagctgggca
     2701 ttgatgaacc ggaaggggct gaaaacttcc gcgaatccat gagtcgggcc atcgagcaat
     2761 gtatagaacg ctatgaaccc cggatctcac acgctgaagt gcaggcagtg gtttcatccg
     2821 cctccagccc gctggatatg acgtttcata ttacagcctg ggtgacattt aacgaaacgc
     2881 atgaggtgct tgaatttgat atggcaccga atggcagcca gcattaccgg gtggactgac
     2941 aaatggaatt tgaagaacgc tattttcggg aagaactcga ttacctgcgc cagcttagca
     3001 agctgctggc aacggaaaaa ccccatctgg cccgcttcct ggccgaaaaa gatgcggatc
     3061 cggatattga acgcctgctg gaaggggtgg cttttcttac cggcaatctc cgccagaaaa
     3121 ttgaggatga attcccagaa ctgacgcacg ggcttattaa gatgctatgg cctaattacc
     3181 tgcgtccggt tccggcaatg acccttattg aatatacgcc ggatatggat aagtcttctg
     3241 taccggtgtt aatcccccgt aatgagcagt ttacaaccaa cgccggggaa atcagagttg
     3301 atgaagtgct gccctctgat gctaaaaagg aggagccgcc tccctgtacc ttcaccctct
     3361 gccgggatat ctggctgctg cccgttcgcc tggagcagat tgaaaaccgc agtacgaccc
     3421 gtaatggtgt tatcaacatc accttttcgg tcgcaccggg aacggatttc cgcacgctgg
     3481 atctgaacaa acttcgcttc tggctcggca atgacgacaa ctatacccgt gaccagcttt
     3541 atttatggtt ctgcgaatac ttgcagggtg ccgacctgac tgtgggtgaa cagcatattc
     3601 gcctgcctga gtttatgcta aaagctgtcg gttttgagcc gcaggatgcc atgctgccct
     3661 ggccgaaaaa cgtccacagc ggctaccgga tccttcagga gtatttctgt taccccgatg
     3721 cgtttctctt ttttaatctt tgtggttgtc cggctttgcc tgacggattg caggcggaat
     3781 tctttaccct gcaactgcgt ttttcgcgcc ctttgcccgt ggacatccgg ctgcgccgcg
     3841 attccctgcg cctgtattgc gcacctgcca ttaatttatt tatccaccat gcagaagcca
     3901 tcacgctgga caaccggcgg gcagactatc cgctggttcc cagccgccat tacccacaac
     3961 attacgatgt attttccgtt aacagtgtgg tgagccaggt ccaggatatg ttcaggaaaa
     4021 aagatctggg gcgtcctgtt tcgacgcagg ccgcgcgcca gtggccagcc tttgaaagtt
     4081 tcagccatca gatggaatac agccggaagc gggaagtggt gtactggcat caccggacca
     4141 aaacatccct gttccatcgc ggctttgatc atacccttgc ctttatacat gctgatggca
     4201 gttatccgtc agacgaatct ctgctcagta atgaagtggt ttcggtatcg ctgacctgta
     4261 ccaaccgtga gcttccgtca caaattcgtt ccggcgatat caccggcaca accggtaaaa
     4321 atgcggctgt tgcttcattt cgcaacatta cccgcccgac gcaaccactc tggccggtca
     4381 ttgatggcag cctgcactgg tccctactct ccgccatgaa cctgaattat ctgtcattac
     4441 tggatacgga cgcgctgaag caggtcatcg ccaactttga tcgccacgca atccatcatc
     4501 cgcagacggc acggctgtca caacaaaagc tggatgccat tgagcgtctg gagacccgcc
     4561 ccgttgatcg cctgtttacg ggtattcccg tccgggggct ggcctccacg ctgtatctgc
     4621 acccggagcc gtttgtctgt gaaggggaaa tgtatctgct cggtacggtg ctttcgcatt
     4681 ttctgtcgct gtacgccagc gttaactcat tccacatgct gaccgttgtg aacacagaaa
     4741 gccaggagac atggaaatgg acggaaagaa tcgggcagca tcctcttatc tgagtccggg
     4801 aaatcccccg gcagataaag agcagaatga tccgctggca caggttttcc ataatgcctg
     4861 ctcttacaat ttttttgcga tggcggagct gttgcaccgc ctggcaaagg gtgaaaaggg
     4921 gacgccagaa ttatccctgc gcgacgatcc ggcacaggaa accctgcgtt tcagcgccga
     4981 tgccagcctt gccttcccgt gcagtgatat cagtgcgctg aaacgggata cgtcaggggc
     5041 attcaggatg accaccacct ttatggggtt acaggggagc cagtcgcctc tccccggtta
     5101 ttacctcgat cacctggcct ggaaagcggt acatgaacaa agtccggtag gtgattttct
     5161 ggatatgttc agccaccgcc tgacccagtt tgtctggcat atctggcgta agtaccgcta
     5221 tcacatcagt tttcgtaacg gcggtgtgga tgcgttctcg cagcgcatgt actcccttgt
     5281 cgggctgggc caccgccagc ttcgcgataa gctggcgatt aatcacagca aaatgctggc
     5341 gtattccggg atcctggcaa atccgggacg atccccggaa attatctgtg gcctggtgtc
     5401 ccactgcttt gatttgtcag aagtgacgct gcaaaactgg caacgtcgca aggtggatat
     5461 tgaaccggat cagcaaaata gtctggggag ctattctctt aaaaatggcg aaaaactggc
     5521 gggaagatcg gtactgggca actttgttct gggtacacaa gtgcccgatc tcagcggaaa
     5581 attccagctc agtattacca gcctgacccg aaaacagttc ctctcttttc tgccgtccgg
     5641 cgaaaacttc ctgccactga cgatgttcgt gtccttcatc ctgcgcgatc agcttgcctg
     5701 ggacttacat ctggggctgg cgccggagca ggtgggcgca atgcgcctgg gcgataacaa
     5761 aagtgcgctg ctgggctgga ccagcttcct cggcacaccg gaagaacgcc cctcagtcac
     5821 catcagggta cggtcgtaaa ttatgataat cacggagcag aatatgccgg aagagaaact
     5881 acagacgctt tcattgcagg tcattaacgg cagtgagctg gaaagcgggc gggcggcgcg
     5941 ctgtctgttc acacagcagg gaaatgtcgg ccatggcccc gaatgccact ggtcggtaca
     6001 ggatcgtcag cagagcattc cggcccaggc ttttaccgtt atcctgcacg atggcacatt
     6061 ttgtctacgc ccgcagacgg cacaactgtg gctgaatcag gcaaaagtca cagcaacatc
     6121 agacctgata cagttgcgcc agggcgatga gatccagatc ggacggctga tggtgagggt
     6181 tcatctgaac cggggagata ttccccatta cgatgaggaa atggccactc ccgaaaccat
     6241 cgttaccaat cgcgatatgc tcacggatac cctgctatca acggagggtg cgccacacta
     6301 tccgggaatg actcaccggc accagcttgc agacaccgtg gtaaacggtt tttctgccga
     6361 tccactccag gcacttcagt ccgaaagcct gattaccacg ggcgatccgc tttcaggcat
     6421 tgcggctgtc cggccatcgg caccgctgtc cgatccggca agtaatgggg ggatcaatac
     6481 tccgtttatg gatctgccgc ccatttatgc cagccccggc gatcgtaatg acgacgtctc
     6541 tgcggcagaa atggcgcaac gccaccttgc ggtcaccccc ttactgcgcg gtcttggcgg
     6601 ctcgcttacc atgagcaatt ccgacgatgc ggatgatttt ctggaggagg ccggacgaac
     6661 gttacaggcc gcaataaaag gtctgctcga tttgcagcag cagcgtaaca gcctctcaga
     6721 caaacatttg cgcccgctgg aagataaccc gctgcgcctg aacatggatt acgccaccgc
     6781 gctcgacgtg atgtttgccg aaggtaaaag cccggtacat ctggcggctc ccgccgccgt
     6841 cagtgaaagc ctgcgcaatg tccgccacca cgaagaagct aaccgggcag caattgtgga
     6901 gtcgcttcgt gtcctgctgg atgctttctc accacaaaat ctgctgcgcc gctttgtgca
     6961 gtaccgccgc agccatgaac tgcgccagcc gctggatgat gccggagcat ggcaaatgta
     7021 cagccattat tacgaagaac tggcctccga tcgccagcag gggtttgaga tgctgtttaa
     7081 cgaggtctac gcccaggtct atgaccgggt gcttcgtgaa aaacagcggg agccggaagc
     7141 atgaacagga aggcgtttct ggcctgcgtg ctgatgtgcg tcatattaac cggctgcgaa
     7201 acggcgaaaa aaatcagcga ggtgatcaaa aacccggata tccaggtcgg gagcctgaag
     7261 gagcaacctt cggaaataac cgtcaccctg ctgactgaac cggacaccaa taccaatgcg
     7321 gagggtgaat cagccgccgt ggatgtgcaa ctggtgtacc tgactgacga ctcaaaattg
     7381 caggcagccg actatgacca gatagccagt acgccgctac cggatgtgct gggtaaaaac
     7441 tatatcgacc accaggattt caacctgttg ccggacacca tcaaaaccct gccgccgata
     7501 aagctggatg agaaaacgca gtttattggt gtcgtggcct atttctccga tgaccaggcc
     7561 actgaatgga aacaaatcga gacggtggaa ggaaccggcc accattaccg cctgctggtg
     7621 catgtacgtc aaagcagcat cgaaatgaaa aaagaggatg aataattatg gcaaccaccc
     7681 gcaataaggt gatgtggcag gaagggatgc tgatgcgccc acaccatttc cagcaacagc
     7741 agcgttacaa cgactacctg gataaccagc gtttccgggc catgaatgat ttatcctggg
     7801 gatttaccga actcaccctc aacaatgaac tgctggcgca gggtaagatc atgattgaca
     7861 gcgcgtcagg cacactgcct gacggcaccg tcttttctat ccccgaccag gacgcactgc
     7921 ccgatccgct gcacccgcaa aattttccgg acgaaagaag ccgcaatatt tacctcgctc
     7981 tgccggtcgc cagtgatgtg agaaatgaaa tcagcgacgg gcggcgaatc gggcgttacc
     8041 ggctgaatta tgccgatgtc cgggatctgc attcagaaga aggcgacgcg cgaacactga
     8101 cgctgggaca actgacgccg cgcattatga gcggtgcaga agatatgagt gcctatatta
     8161 cgctcccgct ttgccgtatc agcgatcgcc atgccgacgg ctccctgacg ctggatgacg
     8221 attttatccc ctcctgccag aatattcagg tcagtaagaa actgcgtgtt tatctcaaag
     8281 aggtacaggg ggccattggc ggacgggcaa gcgatctggc aaaccgtatt ggctctccgg
     8341 cgcagagcgg catcgcggat gtggcggaat ttatgatgtt gcagttgctt aaccgtaacc
     8401 agacccggtt tacccatcgc gcccgccgat cccagctcca cccggaagat ttctaccttg
     8461 atcttgccgg gttgctgggt gaactgatga cctttacaga gccgtcacgc ctgccctgcc
     8521 cgcttgatgt gtatgatcat catgacctga ccaaaacatt taaaacactg ttaccggaag
     8581 tcaaacgggc gctgcatacc gtactgtcgc caagagcggt caatctgccg ctgcatctgc
     8641 gtgacggcat ctggcaggcc gatgttcatg actcggaact gctacagtct gccacctttg
     8701 tactggccgt ggcagcaaat atgcctgtcg atcagatcca gcgtcagttt atccagcagt
     8761 cgaaaatttc ctcgccggaa aaaatccgca atatggtcag tgtgcagatc ccgggtattc
     8821 ctctgcgcgc actgatggtg gccccccgcc agcttcccta ccattccggg ttcagctatt
     8881 tcgaactcga caagagcggc cagacctgga cagaaatggc tgccgccggg gccgttgcac
     8941 tgcatgtatc cggcagtttc ccggatctga acatgcaact gtgggcgata agagggtaag
     9001 acgatggatg aggggtcact gtctctgcca ccatttaccg gatacgacga aaaaagcctg
     9061 cgcgactacc atctcgcgct tcgtggcaac agcctgaatc cgatgatcga cgcggcgacg
     9121 ccgctactcg gtatggtaat gcgcctttcc accatgaaca gccagacgat gccggagcat
     9181 ctttttgctc aggtggtgac agatgttcag gccgtggagc aactgcttca ggaacagggc
     9241 tacgaaccgg gcgtgattat ctcgtttcgc tacatcctct gcacgtttat tgatgaagcg
     9301 gcgctcggta acggctggtc gaacaagaac gagtggatca agcagtcatt gctggtccat
     9361 ttccacaacg aagcctgggg tggcgagaaa gttttcatcc tgctggagcg cctgatccgt
     9421 gaacccaaac gttaccagga tgtgctggaa tttctctggc tctgtttttc tctgggattt
     9481 cgcgggcgct acaaagtggc agtacaggat cagggggaat ttgagcagat ctaccgccgt
     9541 ctgtaccacg ttctgcataa actgcgcggc gatgcccctt tcccgctgtt acaccaggac
     9601 aaaaaaaccc agggcgggcg ttatcagctc atcagtcgcc tgactgttaa acatattttt
     9661 tgtggtggcg ttgtcgtgct ggcgttgttt tacctgttct atctgttgcg gctggacagc
     9721 cagacgcagg acatcctgca ccagctaaac aaattacttc gataaggacg tttatgatcc
     9781 agattgatct tcccacgctg gtaaaacggc tgaacctgtt ctcccgccag gcgctggaga
     9841 tggccgcctc tgaatgtatg agtcagcagg ctgcggaaat taccgtcagc catgtgctta
     9901 ttcagatgct cgccatgcca cgcagtgacc tgcgggttat tacccgccag ggcgatattg
     9961 gcatggaaga gttgcgccag gcgttgacgg tagagaacta cacaaccgcc cgttctgcgg
    10021 acagctaccc ggcgttttcc ccgatgctgg ttgagtggct taaagagggc tggctgctgg
    10081 cgtcggctga gatgcagcac agcgaactgc gcggcggcgt gttgctgctg gccttgctgc
    10141 attcgccgct gcgttatata ccgcctgctg ccgcccggct gttgaccggc attaaccgtg
    10201 accgtctgca acaggacttt gtgcagtgga cacaggagtc ggcggaatca gtcgtgccgg
    10261 atgcagacgg taaaggcgca ggcacactga cggacggctc tgacaccctg cttgcccgct
    10321 acgccaaaaa catgaccgca gacgcccgta acggcaggct agacccggta ctgtgccgcg
    10381 accacgaaat cgacctgatg atcgacattc tctgccgccg ccgtaaaaac aacccggtgg
    10441 tggtgggcga agcaggcgtg ggtaaaagcg cgctgattga agggctggcg ctgcgcatcg
    10501 tggcaggcca ggtgccggac aaactgaaaa acaccgatat catgaccctt gacctgggcg
    10561 cattgcaggc cggggcatcg gtgaagggtg agtttgaaaa acgcttcaag gggctgatgg
    10621 cggaggtcat ttcctctccg gtgccggtca ttctgtttat cgacgaagcg cataccctga
    10681 ttggcgcggg caaccagcag ggcgggctgg atatctccaa cctgctgaaa ccggcgctgg
    10741 cgcgtggcga actgaaaacc atcgccgcca ccacctggag cgagtacaaa aaatacttcg
    10801 agaaagacgc cgccctgtcg cgccgcttcc agttagtgaa ggtcagcgaa cccaacgctg
    10861 ccgaagccac cattattctg cgtggtctgt cggcggtcta tgaacggtct cacggcgtgc
    10921 tgattgacga tgatgccttg caggccgcag caacgctgag cgaacgttat ctctccgggc
    10981 gtcagttacc ggacaaagcg attgatgtgc tggataccgc ctgcgcccgt gtggccatca
    11041 acctgtcgtc gccgccgaag caaatctcgg cgctgaccac tctgagccac cagcaggagg
    11101 cggaaattcg ccagcttgag cgcgagcttc gcatcggact gcgtaccgac acatcacgga
    11161 tgaccgaggt gctggtgcag tatgatgaaa cgctgacggc gctggatgaa ctggaagcgg
    11221 cctggcacca gcagcagacg ctggtccggg agattatcgc gctgcgccag cagttactgg
    11281 gcgtggcaga ggacgatgcg gcgccgttgc cggacgcaga taccgtggag gatacgcagc
    11341 cagagtcaga gtcagagtca gaacaggata ataccggtgc cgtaccggct gatgaggccg
    11401 acagagaaca gccggaagag accgctgaaa cagtttcccc ggtacagcgg ctggcccagc
    11461 tcactgccga actggacgcc ctgcataacg accggttgct ggtctccccg cacgtcgata
    11521 aaaaacagat tgcggcggtg attgccgaat ggaccggcgt gccgctcaac cgcctgtcgc
    11581 agaatgaaat gtcggtcatc accgacctgc cgaaatggct gggcgacacc atcaaaggcc
    11641 aggacctggc gattgccagc ctgcataagt atctactgac cgcacgcgcc gacctgcgtc
    11701 gtccgggacg cccgctcggt gcgttcctgc tggctggccc cagcggtgtg ggtaaaaccg
    11761 aaaccgtcct gcaactggca gagctgctct acggcggtcg ccagtacctg accaccatca
    11821 atatgtccga attccaggag aaacacaccg tctcgcggct gattggttcg cctccgggct
    11881 acgttggcta cggtgaaggc ggcgtactga ccgaagcgat tcgccagaaa ccctactcgg
    11941 tagtactgct cgatgaagtg gaaaaagcgc acccggatgt gctcaacctg ttctaccagg
    12001 cgttcgataa gggcgaaatg gcagacggtg aaggccgcct gattgactgc aaaaatatcg
    12061 tcttcttcct gacgtccaac ctcggctacc aggtaatagt cgagcatgcc gatgacccgg
    12121 aaaccatgca ggaagtactg tatccggtgc tggccgactt cttcaaacct gccctgctgg
    12181 cgcgtatgga agtggtgccg tatctgccgc tgtcgaaaga gacgctcgcc accattatcg
    12241 ccgggaaact ggcccgtctg gataacgtgc tgcgcagtcg ctttggtgca gaagtggcca
    12301 ttgaaccgga agtgacggac gaaatcatga gccgcgtcac ccgcgcggaa aacggcgcga
    12361 ggatgctgga atcggtcatc gatggcgaca tgctaccgcc gctctcgctg cagctgttgc
    12421 agaaaatggc ggctaacacg gcgattgccc ggattcggtt gtcggcagtg gacggcgcat
    12481 ttacggcaga cgtggaagat gctcagaacg acgagtccgt cacaaaggat gaaacggttt
    12541 tatgagcagg ttgcgcgttt tccggcagac aggatggttt atcgcgggcc tgatgacggg
    12601 cttacccgcc actgccgcgc ccgcagaagc atcgtcaatg gccagtgtgg cggtcgccgt
    12661 cgccaccacg accccgccgg atgcgacagc cacgttacag gccatgcagt cctgtcggcg
    12721 ggagtccgcg gcgctggagc ggcttgactg ctatgaccgc ctgctggcac cgctgtcccc
    12781 gtcagggttt gacggggcgc tggtcaaagc cggttttgtg ggcgaggcct ggacccgcgc
    12841 cacagagcag gaaaaacgcc gcgagggcaa caccacagaa ctgctggtga cgcaggtgcc
    12901 gggggagcgc ccgacggtgg tgataaccac cccggctatc ggccatgttc cgccgcgccc
    12961 ggtgctgatg ttcagttgcg tggacaatat cacccggatg caggtggcgc tgatgcaccc
    13021 tctggatgtc catgatatcg ccgtcactct gaatgccgac agccgggcat tgcgcagcca
    13081 ctggtttgtg cgcgagaacg gcaccctgct ggagtccagc cgggggctgt ccgggattga
    13141 tgaaattaag caactgttcg gcgcaaaaac gctgacagtg gataccggcg cagataacgc
    13201 cgcaggaaag ctgaccttta acattgacgg gctggcaagg gcgattgccc ccctgcgcga
    13261 tgcctgccac tgggcgggag aataaaaata tggcaatgga cttacgcgat ccgaatgtct
    13321 ggatatcgca cctgctggaa aacctgccag aagaaaaact ggcatcggcg ctgaaagatg
    13381 acaacccgaa ctgggagtat atcgacggcg aaatcgtcaa actgggatct ctggcccaca
    13441 gtcaactgga tatcccggaa ctccagcgcc gggggctggt cattctggct tcggagagta
    13501 aggactttcg tctgctggcg catctgctga gaaccctgca acacgccggt gatcctcatc
    13561 tcgcattgcg cctgctggcg ctgtatgtgg agcattactg gtcggtggct gcgccgcaga
    13621 atatggcaca taaaaaacgc tttgccagcc aggtcattaa acgctttgaa acaggtattg
    13681 aaggcttttc acagaacgcc gccacagcgc agcgcgacgc gctgtcaggg gaactggcga
    13741 aactggcgca gtgctggcag tcacataacg ccccggaact ggcactggct accgatgatc
    13801 tttttgccct gtaccagcgt gcgtttaatc gtgcggcacc ggctcctgcc cccactccgg
    13861 cggcctccga tagttcacca cagaccaccg ccacgtctga aagcggcgtg acgcaaccca
    13921 gtgctccgac tccccaaatc gccatcgaca gtcacgacga caaagcctgg cgcgacacgc
    13981 tgttaaaagt ggcggctatt ttatgtgaac gccagccgga ctcgccgcag ggctatcgcc
    14041 tgcgccgcca tgccctgtgg caatccatca ccagtacacc ccaggcggaa agcgatggac
    14101 gtaccccatt ggctgcggtc tctgccgata tggtggcgga ttaccagtcc cggcttgcca
    14161 gcgcggatat ggcgctgtgg cagcaggttg agaaaagcgt attgctggct ccttactggc
    14221 tggacggtca ctgtctttct gcacagacgg cactgcgtct gggttacaaa caggtggcag
    14281 acaccatccg cgatgaggtc atccgcttcc ttgagcgtct gccccagctt accgggctgc
    14341 tgtttaatga ccgcacaccg tttctcagtg agcagacgaa acaatggctg gctgcttcgc
    14401 ccgacggcaa agttgcaccg gttgcgcaaa tcggtgagga atcgcaggca gccagagcct
    14461 gttttgctgg gcagggtctg gaggcggcgc tgcgatatct ggacatgcta cccgaaggcg
    14521 atccccgcga tcagtttcac cgccagtacc ttgccgcaca gttgacggag gaggcggggc
    14581 tgatacagct tgcgcagcaa cagtaccgga tgttgttgat gatagggagt cagatgatgg
    14641 tgtctgactg ggagccatca ttacttacgc agcttgaaca aaaattcacg gcagaacaat
    14701 aaaagagaac aggagttttt gtgttcagat taccaacacc ccgactactc agcggactca
    14761 aatcagccct acgcccggcg atgccccggt ttaaagtctc cgctttctgg ctgctgatac
    14821 tggcgtggat ttttctgctg gtatggatct ggtggaaagg ctcgacgtgg acgctgtatg
    14881 aagaacagtg gctcaaacca ctggcgaacc gctggctggc aacggcggca tgggggatta
    14941 tcgccctggt gtggcttacc gtccgggtga tgaagcgcct gcaacaactt gaaaaaatgc
    15001 aaaagcagca gcgcgaggag gccattgatc cgctcagtgt ggagctgaac gcccagcagc
    15061 gttaccttga ccgctggctg ctgcgcctgc aacgccatct cgacaaccgc cgtttcctgt
    15121 ggcagttgcc gtggtatatg gtcatcggcc cggcgggcag tggcaaaacg acactgctgg
    15181 gcgaggggtt tccgtcagac attatttatg ccccggaagg cgcgcgcggc acagaacaac
    15241 gcctgtacct tacgccccat gtcggtaaac aggcggtgat ctttgatatc gacggcacac
    15301 tctgcgctcc cgctgatgcg gatatcctgc atcgccgtct gtgggaacat gccctcggct
    15361 ggctgaaaga aaagcgcgcg cgccagccgc tgaacgggat tattctgaca ctcgatttac
    15421 ccgatctgct taccgcagac aaacgccgcc gcgagcatct gttacagacg ctgcgcagcc
    15481 gcttgcagga tatacgccag catcttcact gccagttacc ggtttacgtg gtactcaccc
    15541 ggcttgattt attgcagggt tttgccgccc tgttccagtc actgaacaga caggatcgcg
    15601 atgcgattct gggcgtcacg ttcacccgcc gcgcccatga aaatgatgac tggcgaacag
    15661 agttgaatgc tttctggcag acatgggtgg atcgaatgaa cctagcgttg ccggatctga
    15721 tggtcgctca gactcacacc cgcgcgtctt tatttagttt ttcccgccag atgcagggaa
    15781 gccgtgaacc gctggtgtca ctgcttgagg gtctacttga tggcgaaaat atgaacgtga
    15841 tgctgcgtgg tgtctatctc acctcttcgc ttcagcgtgg acagatggat gatatattca
    15901 cccagtctgc cgcccgccag taccggctgg gcaataaccc actggcgtcc tggcctctgg
    15961 tggacaccgc gccttatttc acccgcagct tgttcccgca ggcattactc gcagagccta
    16021 atctggcaac ggagagccgc gcctggctga tgcgttcccg tcgccgcctg acggttttct
    16081 ctaccacagg aggcgtggcg gcactgctgc tcatcaccgg ctggcatcac tattacaacg
    16141 gtaactatca gtccggcatc accgtgctta agcaggccaa agcctttatg gatgtgccgc
    16201 ctccacaggg ggaagatgac tacggcaacc tgcaactgcc gctgctgaac ccggtacgcg
    16261 atgccacact ggcctatggc gactggggcg accgcagccg tctggccgat atgggactgt
    16321 accagggacg acgtatcggg ccttatgtgg aacagaccta tctgcaactg ctggagcaac
    16381 gttacctgcc ctcgctgttt aacgggctgg ttaaagcgat gaatgccgcg ccgccggaga
    16441 gtgaagaaaa actcgcggtg ctgcgcgtga tgcgaatgct ggaggacaaa agcggacgta
    16501 acaatgaggt ggtgaagcag tatatggcaa aacgctggag cgaaaagttc cacggccagc
    16561 gcgatatcca ggcacaactg atgtcccatc ttgactacgc gctggctcat actgactggc
    16621 acgcagagcg tcaggcgggc gacggtgacg ccatcagccg ctggacgcca tatgacaagc
    16681 ccgtggtatc agcacagaaa gaactgagca aactgcctgt ctaccagcgg gtttaccaga
    16741 gcctgaaaac gcgggcgctg ggcgttcttc ctgccgacct caatctgcgt gaccaggtag
    16801 ggccaacctt tgaccaggtg tttacatctg ccgatgacaa caaactggtt gttccacagt
    16861 ttcttacccg ttacggcctg caaagctatt ttgtaaaaca gcgcgatgaa ctggttgaac
    16921 tgacggcgat ggattcctgg gtacttaacc ttacccgcag cgtgaaatac agtgacgccg
    16981 accgcgcgga aatccagcgc cagctcaccg agcagtatat cagcgactac accgccacct
    17041 ggcgggccgg gatggacaat ctgaatatcc gcaattttga gtccatcgga caactgaccg
    17101 gggcgctgga gcaggttatc agcggcgacc agcctttgca gcgggcgctg accgtgctgc
    17161 gtgacaacac acagccaggc gtcttttctg aaaaactctc tgccaaagaa cgggatgagg
    17221 ccctggcaga gccggactac cagttactca cccgtctcgg gcatgagttc gccccggaaa
    17281 acagtaccct ggcagtacag aaagacaaag aaagcacgat gcaggcagta tatcagcaac
    17341 tcaccgagtt gcaccgctac ctgctggcaa tccagaacgc gcctgtacca gggaaatcgg
    17401 cgctgaaagc cgtgcagtta cggcttgatc agaacagcag cgatccgata ttcgccaccc
    17461 gccagatggc aaaaacgctg cctgctccgc tcaaccgctg ggttggcagg ctggctgacc
    17521 aggcctggca tgtggtgatg gtggaggccg ttcactatat ggaagtggac tggcgcgaca
    17581 gcgtggtgaa accgtttaac gagcaactgg caaataacta tccgtttaat ccgcgttctg
    17641 cacaggatgc ctcactggat gccttcgaac gcttctttaa accggatggc atactggata
    17701 ccttctacca gcagaacctg aagctgttta tcgataatga cctgagtctg gaggatggcg
    17761 ataacagcgt cattattcgc gaagatatta ttgcgcaact ggaaactgcg cagaaaatcc
    17821 gtgacatctt cttcagcaaa cagaacggtc tgggaacatc ctttgccgtg gaaacggtat
    17881 cgctttcagg caataaacgc cgcagtgtac tgaaccttga cggtcagtta gtcgattaca
    17941 gccagggccg taactatacc gcccatctgg tctggcctaa caacatgcgc gaaggcaacg
    18001 aaagtaagct gacgctcatc ggcaccagcg gcaacgcgcc gcgcagtatc agcttcagcg
    18061 ggccgtgggc gcagttccgc ctgttcgggg ccggacaact gaccggagta caggatggca
    18121 actttaccgt gcgctttagc gtggacggtg gcgcgatgac ctaccgtgtg cataccgaca
    18181 cggaagataa cccgttcagc ggtgggttgt tcagccagtt tggtctgtca gacacactgt
    18241 actgaggagg cagggatgaa tagtaacgta ctgacacaaa ctatcgttac cggcagtgac
    18301 ccgcgcgggc tgccggaatt cagcgccatc cgcgaggaaa taaacaaagc cagccacccg
    18361 tcacagcctg agctgaactg gaaactggtg gagtcgctgg cgctggcgat ttttaaagcc
    18421 aacggtgtgg atttacacac cgccacctac tatacgcttg cccggacacg gacacaggga
    18481 ctggcgggat tctgcgaagg tgcggaactg ctggcggcaa tggtaagcca cgactgggat
    18541 aagttctggc cgcagggcgg cccggcgcgt actgaaatgc tggactggtt taactcccga
    18601 accggcaata ttctgcgtca gcaaatctcc tttgcggaat ctgatctgcc gctgatctac
    18661 cgcacagagc gggcattgca gcttatctgc gacaagcttc agcaggtaga gctgaagcgc
    18721 gttccgcgcg tggagaatct gctctatttt atgcagaaca cgcgtaaacg gcttgaaccc
    18781 cagctgaaga gtaacactga gaacgccgca cagaccacgg tcagaacgct gatttatgcc
    18841 ccggaaacac agccatcttc cacaccagaa gcggtagtgc ctcccctgcc cggcctgcct
    18901 gagatgaaag tggaagtgcg cagtctgaca gagaatcccc cacaggccag tgtgataaag
    18961 caaggcagta cggtaagagg gtttatcgca gggatcgcct gttcagtggc tgtcgcctca
    19021 gcattgtggt ggtggcaggt ctatccggtg cagcagcaac tgttacaggt taacgacacc
    19081 gctcagggcg cagcaacggt gtggatggcc tcacctgaac tcgaaaacta tgagcgcagg
    19141 ctgcaacaac ttcttgatac ctccccggta cagccgctgg aaaccgggat gcagatgatg
    19201 cgtgttgccg acagtcgctg gccggaaagc ctgcaacagc aacaggcctc gacacaatgg
    19261 aatgaggcac tcaaaacccg cgcacagaat agcccgcagt tgcgtggctg gttgcagacc
    19321 cgccaggact tacatgcttt tgcagatcta gtgatgcagc gcgagaaaga gggactcacc
    19381 ctttcctata tcaaaaatgt catctggcag gcggagcggg gactggggca ggaaacaccc
    19441 gttgagtctc tgttgacgca gtaccaggat gcccgtgcgc agaagcagaa tacagatgcg
    19501 ctggaaaaac aaattaatga gcgactcgaa ggcgtgttaa gccgctggct gctgctgaat
    19561 aataatacga taccgacgat aaaaaaaggc attgaatttc aataacattc atgaatacaa
    19621 aggagtttta aatggcgaat ttaatttatt taacactaaa tggtg
//
LOCUS       DAPGOF010000088        19524 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.088, whole genome shotgun sequence.
ACCESSION   DAPGOF010000088 DAPGOF010000000
VERSION     DAPGOF010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.088"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(360..842)
                     /locus_tag="RHV13_002733"
     CDS             complement(360..842)
                     /locus_tag="RHV13_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HDV1242254.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            1078..3660
                     /gene="leuS"
                     /locus_tag="RHV13_002734"
     CDS             1078..3660
                     /gene="leuS"
                     /locus_tag="RHV13_002734"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDV1242255.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPDYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPQY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVD"
     gene            3675..4256
                     /gene="lptE"
                     /locus_tag="RHV13_002735"
     CDS             3675..4256
                     /gene="lptE"
                     /locus_tag="RHV13_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HDV1242256.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            4256..5287
                     /gene="holA"
                     /locus_tag="RHV13_002736"
     CDS             4256..5287
                     /gene="holA"
                     /locus_tag="RHV13_002736"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDV1242257.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            5289..5930
                     /gene="nadD"
                     /locus_tag="RHV13_002737"
     CDS             5289..5930
                     /gene="nadD"
                     /locus_tag="RHV13_002737"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDV1242258.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            5954..6565
                     /locus_tag="RHV13_002738"
     CDS             5954..6565
                     /locus_tag="RHV13_002738"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HDV1242259.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLHD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILVVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRANGVENA"
     gene            6825..7142
                     /gene="rsfS"
                     /locus_tag="RHV13_002739"
                     /gene_synonym="rsfA"
     CDS             6825..7142
                     /gene="rsfS"
                     /locus_tag="RHV13_002739"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDV1242260.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            7146..7613
                     /gene="rlmH"
                     /locus_tag="RHV13_002740"
     CDS             7146..7613
                     /gene="rlmH"
                     /locus_tag="RHV13_002740"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDV1242261.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            7644..9545
                     /gene="mrdA"
                     /locus_tag="RHV13_002741"
     CDS             7644..9545
                     /gene="mrdA"
                     /locus_tag="RHV13_002741"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HDV1242262.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            9548..10660
                     /gene="mrdB"
                     /locus_tag="RHV13_002742"
                     /gene_synonym="rodA"
     CDS             9548..10660
                     /gene="mrdB"
                     /locus_tag="RHV13_002742"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HDV1242263.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            10671..11759
                     /gene="rlpA"
                     /locus_tag="RHV13_002743"
     CDS             10671..11759
                     /gene="rlpA"
                     /locus_tag="RHV13_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HDV1242264.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            11899..13110
                     /gene="dacA"
                     /locus_tag="RHV13_002744"
     CDS             11899..13110
                     /gene="dacA"
                     /locus_tag="RHV13_002744"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HDV1242265.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            13220..13483
                     /locus_tag="RHV13_002745"
     CDS             13220..13483
                     /locus_tag="RHV13_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HDV1242266.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            13584..14225
                     /gene="lipB"
                     /locus_tag="RHV13_002746"
     CDS             13584..14225
                     /gene="lipB"
                     /locus_tag="RHV13_002746"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HDV1242267.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            14485..15438
                     /locus_tag="RHV13_002747"
     CDS             14485..15438
                     /locus_tag="RHV13_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HDV1242268.1"
                     /translation="MDSNNQIESCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRIQEMFPDRQISFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            15647..16612
                     /gene="lipA"
                     /locus_tag="RHV13_002748"
     CDS             15647..16612
                     /gene="lipA"
                     /locus_tag="RHV13_002748"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HDV1242269.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(16713..16916)
                     /gene="tatE"
                     /locus_tag="RHV13_002749"
     CDS             complement(16713..16916)
                     /gene="tatE"
                     /locus_tag="RHV13_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HDV1242270.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(17045..17833)
                     /locus_tag="RHV13_002750"
     CDS             complement(17045..17833)
                     /locus_tag="RHV13_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HDV1242271.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFASPQLL"
     gene            17926..18309
                     /gene="crcB"
                     /locus_tag="RHV13_002751"
     CDS             17926..18309
                     /gene="crcB"
                     /locus_tag="RHV13_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HDV1242272.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(18362..18571)
                     /gene="cspE"
                     /locus_tag="RHV13_002752"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(18362..18571)
                     /gene="cspE"
                     /locus_tag="RHV13_002752"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HDV1242273.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(18746..19306)
                     /gene="pagP"
                     /locus_tag="RHV13_002753"
     CDS             complement(18746..19306)
                     /gene="pagP"
                     /locus_tag="RHV13_002753"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HDV1242274.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT         4912 a         4971 c         4851 g         4790 t
ORIGIN      
        1 tcaataatat ccgttgatgt aatattgcta tttttgagca tcgagcggcc cagaaaacca
       61 taaatctctg ctactttctc tgacgcattt gacataacat ccctcttaaa tcaatctaaa
      121 actaatgcta aatatttaaa atattgatga ttcggtagaa tgcatattaa aaggaggaag
      181 agcgcctggc tctccctcac gtgctgttaa aaaagttgaa ctaaaaatga tagatagtaa
      241 caacgctacg ctgacagcca ctcttcccat gtcgacattc ctgaatgtat tccctttttc
      301 ttcattccaa cagaaatggt attcgacata aatcgctaac agcgtgcgaa atcagactat
      361 tacggctcaa acgggcgtct ctggaactgg tcatgaccac atttcgggca tagcgtcagc
      421 acttccggtg tgtagatcgg gagatggaag tgacatttct cgcagaccag atttcccagc
      481 ccgaccactt ctccgctgtg ataaacccca tgatgattga ggtcctggaa aacttcgcgc
      541 cattcaagct gcgttttatc ggtgatgtct gccagctcct gccacaagct ttctttaatc
      601 acccgcataa agacgctgtc agattcttct ttcaggctct cttcatagct catggcgaac
      661 tcttccaggt cacgtctgac agctcgcgtc agctcatcga cctcggttcg cgttaactcc
      721 cctgttttta ttacgcgctc gcgcgcctgt tccaccagtg cgtcgatatc acgttcgcca
      781 ttgcgcaggc gttcgctcag tgacgcaacc agttcacggt aatattgagc aaccttgttc
      841 atcgtttcat ctcccgggtc gttaactgac tataataata gacgttcttt gttccttgct
      901 ttgctaatac gcccacaaac tctgtaaatt aatgtatgaa taatccgggc atcatttcaa
      961 cgtcgagtgc gcgaaaggct gttttgacgc ggacgtttgg gctatgctat gcggatctga
     1021 aaaaccacct ctaacgctac ttttgtagcc gtaatgaaaa caggaccact ggctgccatg
     1081 caagagcaat accgcccgga agagatagaa tccaaagtac agcttcattg ggatgagaag
     1141 cgcacatttg aagtaaccga agacgagagc aaagagaagt attactgcct gtctatgctt
     1201 ccctatcctt ctggtcgact acacatgggc cacgtacgta actacaccat cggtgacgtg
     1261 atcgcccgtt accagcgcat gctgggcaaa aacgttctgc agccgatcgg ctgggacgcg
     1321 tttggtctgc ctgcggaagg cgcggcggtg aaaaacaaca ccgctccggc accgtggacg
     1381 tacgacaaca tcgcgtatat gaaaaaccag ctcaaaatgc tgggctttgg ttatgactgg
     1441 agccgcgagc tggcaacctg tacgccggat tactaccgtt gggaacagaa attcttcacc
     1501 gagctgtata aaaaaggcct ggtatataag aagacttctg cggtcaactg gtgcccgaac
     1561 gaccagaccg tactggcgaa cgaacaagtt atcgacggct gctgctggcg ctgcgatacc
     1621 aaagttgaac gtaaagagat cccgcagtgg tttatcaaaa tcaccgctta cgctgacgag
     1681 ctgctcaacg atctggataa actggatcac tggccagaca ccgttaaaac catgcagcgt
     1741 aactggatcg gtcgttccga aggcgtggaa atcaccttca acgttaacga ctatgacaac
     1801 acgctgaccg tttacactac ccgcccggac acctttatgg gttgtaccta cctggcggta
     1861 gctgcgggtc atccgctggc gcagaaagcg gcggaaaata atcctgaact ggcggccttt
     1921 attgacgaat gccgtaacac caaagttgcc gaagctgaaa tggcgacgat ggagaaaaaa
     1981 ggcgtcgata ctggctttaa agctgttcac ccattaacgg gcgaagaaat tcccgtttgg
     2041 gcagcaaact tcgtattgat ggagtacggc acgggcgcag ttatggcggt tccgggtcac
     2101 gaccagcgcg actacgagtt tgcctctaaa tacggcctga acatcaaacc ggttatcctg
     2161 gcggctgacg gctctgagcc agatctttct cagcaagccc tgactgaaaa aggcgtgctg
     2221 ttcaactctg gcgagttcaa cggtcttgac catgaagcgg ccttcaacgc catcgccgat
     2281 aaactgactg cgatgggcgt tggcgagcgt aaagtgaact accgcctgcg cgactggggt
     2341 gtttctcgtc agcgttactg gggcgcgccg attccgatgg tgacgctgga agacggtacc
     2401 gtaatgccga ccccggacga ccagctgccg gtgatcctgc cggaagatgt ggtgatggac
     2461 ggcattacca gcccgattaa agcagatccg gagtgggcga aaactaccgt taacggtatg
     2521 ccagcactgc gtgaaaccga cactttcgac acctttatgg agtcctcctg gtactatgcg
     2581 cgctacactt gcccgcagta caaagaaggt atgctggatt ccgaagcggc taactactgg
     2641 ctgccggtgg atatctacat tggtggtatc gaacacgcca ttatgcacct gctctacttc
     2701 cgcttcttcc acaaactgat gcgtgatgca ggcatggtga actctgacga accagcgaaa
     2761 cagttgctgt gtcagggtat ggtgctggca gatgccttct actatgttgg cgaaaacggc
     2821 gaacgtaact gggtttcccc ggttgatgct atcgttgaac gtgacgagaa aggccgtatc
     2881 gtgaaagcga aagatgcggc aggccatgaa ctggtttata ccggcatgag caaaatgtcc
     2941 aagtcgaaga acaacggtat cgacccgcag gtgatggttg aacgttacgg cgcggacacc
     3001 gttcgtctgt ttatgatgtt tgcttctccg gctgatatga ctctcgaatg gcaggaatcc
     3061 ggcgtggaag gcgctaaccg cttcctgaaa cgtgtctgga aactggtgta cgagcatact
     3121 gcgaaaggtg atgttgcggc actgaacgtt gatgcactga ctgaagatca gaaagcgctg
     3181 cgtcgcgatg tgcataaaac tatcgctaaa gtgaccgatg atatcggccg tcgtcagacc
     3241 ttcaacaccg caattgcggc gattatggag ctgatgaaca aactggcgaa agcaccgacc
     3301 gatggcgagc aggatcgcgc tctgatgcag gaagcgctgc tggccgttgt ccgtatgctt
     3361 aacccgttca ccccgcacat ctgcttcacg ctgtggcagg aactcaaagg cgaaggcgat
     3421 atcgacaacg cgccgtggcc ggttgctgac gaaaaagcga tggtggaaga ctccacgctg
     3481 gtcgtggtgc aggttaacgg taaagtccgt gctaaaatca ccgttccggt ggacgcaacg
     3541 gaagaacagg ttcgcgaacg tgctggccag gaacatctgg tagcaaaata tcttgatggc
     3601 gttactgtac gtaaagtgat ttacgtacca ggtaaactcc tcaatctggt cgttgactaa
     3661 gcgcgggagg aagcgtgcga tatctggcaa cattgttgtt atctctggcg gtgttaatca
     3721 ccgccgggtg tggctggcat ctgcgtgaca ccacgcaggt tccttccact atgaaggtca
     3781 tgatcctgga ctcaggcgat ccgaacgggc cattaagccg tgcggtgcgt aaccagttac
     3841 gtctgaatgg tgtcgagttg cttgataaag aaaccacgcg taaggacgtt ccatccctgc
     3901 gtttgggtaa agtgagcatc gcgaaagata ccgcatcggt attccgtaac ggtcaaacag
     3961 cagagtatca gatgatcatg acggtgaatg cgaccgtgtt gatccccggc cgtgatatct
     4021 acccgattag cgccaaagtc ttccgttcgt tctttgataa cccgcaaatg gcgttagcga
     4081 aagataacga acaagacatg atcgtaaaag agatgtacga ccgtgctgcc gaacagctga
     4141 ttcgtaagct gccaagcatc cgtgctgcgg atattcgttc cgacgaagaa cagacgtcga
     4201 ccacaacgga tactccggca acgcctgcac gcgtctccac cacgctgggt aactgatgat
     4261 tcggttgtac ccggaacaac tccgcgcgca gctcaatgaa gggctgcgcg cggcgtatct
     4321 tttacttggt aacgatcctc tgttattgca ggaaagccag gacgctgttc gtcaggtagc
     4381 tgcggcacaa ggattcgaag aacaccacac tttttccatt gatcccaaca ctgactggaa
     4441 tgcgatcttt tcgttatgcc aggctatgag tctgtttgcc agtcgacaaa cgctattgct
     4501 gttgttacca gaaaacggac cgaatgcggc gatcaatgaa caacttctca cactcaccgg
     4561 acttctgcat gacgacctgc tgttgatcgt ccgcggtaat aaattaagca aagcgcaaga
     4621 aaatgccgcc tggtttactg ctcttgcgaa tcgcagcgtg caggtgacct gtcagacacc
     4681 ggagcaggct cagcttcccc gctgggttgc tgcgcgcgca aaacagctca acttagaact
     4741 ggatgacgct gcaaatcagg tgctctgcta ctgttatgaa ggtaacctgc tggcgctggc
     4801 ccaggcactg gagcgtttat cgcttctctg gccagacggc aaactgacat taccgcgggt
     4861 tgaacaggcg gtgaatgatg ccgcgcattt cacccctttt cattgggttg atgctttgtt
     4921 gatgggaaaa agtaagcgcg cattgcatat tcttcagcaa ctgcgtctgg aaggcagcga
     4981 accggttatt ttgttgcgca cattacaacg tgaactcttg ttactggtta acctgaaacg
     5041 ccagtctgcc catacgccac tgcgtgcgtt gtttgataag catcgggtat ggcagaaccg
     5101 ccggggcatg atgggcgagg cgttaaatcg cttaagtcag ccgcagttac gtcaggccgt
     5161 gcaactcctg acacgaacgg aactcaccct caaacaagat tacggtcagt cagtgtgggc
     5221 agagctggaa gggttatctc ttctgttgtg ccataaaccc ctggcggacg tatttatcga
     5281 cggttgatat gaaatcttta caggctctgt ttggcggcac ctttgatccg gtgcactatg
     5341 gtcatctaaa acccgtggaa acgctggcga atttgattgg tctgacgcgg gtcacaataa
     5401 tccctaataa tgttcctccg catcgtcccc agccggaagc gaacagcgta cagcgtaaac
     5461 acatgcttga actggcgatt gccgacaagc cattatttac tcttgatgaa cgagagctaa
     5521 agcgcaatgc cccctcttac actgcgcaaa cactgaaaga gtggcggcag gaacaaggac
     5581 cggacgtgcc gctggcgttt attattggtc aggattcatt gctgaccttt ccgacctggt
     5641 acgaatacga aacgatactc gacaatgcac atttgatcgt ctgtcggcgt ccaggttacc
     5701 cacttgaaat ggcgcaaccg caataccagc aatggctgga agatcatttg acacataacc
     5761 cggaagatct tcaccttcag cctgccggta aaatttatct ggctgaaacg ccgtggttta
     5821 acatctcggc gaccatcatc cgcgaacgtt tgcaaaacgg tgaatcgtgt gaggatttat
     5881 tgccggaacc ggtattgact tacattaacc aacaaggctt gtatcgctga tacctgtcgt
     5941 tacggagggc atgatgcgac tgtggttaat tcgtcatggc gaaacacaag cgaatgtcga
     6001 tggtctgtac agcggtcatg cgcccacccc cctgaccgca cgcggtattg agcaagcgca
     6061 aaatctgcat acactgctac atgatgtttc ctttgacctg gttttatgca gtgaactgga
     6121 acgggcacag cataccgcgc gactggttct cagtgaccgc cagctccccg ttcatatcat
     6181 ccctgaactc aacgaaatgt tttttggcga ctgggagatg cgacatcatc gcgacctcat
     6241 gcaagaagat gccgaaaact atagcgcgtg gtgcaatgac tggcagcatg ctatccccac
     6301 gaacggtgaa ggatttcagg cattttcaca acgtgtggaa cgctttatcg caaggcttag
     6361 tgaatatcag cactatcaga atattttagt cgtcagccat cagggcgtac tgagtctgtt
     6421 aattgctcgt ctaattggca tgcctgccga gtctatgtgg cattttcgcg ttgaccaggg
     6481 gtgttggagc gccattgata tcaaccaaaa attcgcgacg ctacgtgtcc tcaatagccg
     6541 tgccaacggg gtcgagaatg catgactttt ctgttttttt acgggtaagc cgcaacgacc
     6601 attgacagcc ccgggcaggc tgatattctc cgcagccaga ctttttccgc cagacacgac
     6661 tttgtagaaa ttgttttaca aaaatggcga tgcaatctgc ggcgcggggt gggatgatag
     6721 cccactttcg aaagccgatt cggcgacaat tgtcccgaaa tcgcctctgg ttcaggtata
     6781 ctgacagacc atttttatct atttgattca cccaggggga aaacttgcag ggtaaagcac
     6841 tccaggattt tgttatcgac aaaattgatg acctcaaagg tcaggacatc atcgccttag
     6901 acgttcaggg caaatccagc atcactgact gcatgatcat ctgtacgggt acgtccagcc
     6961 gtcatgttat gtccattgct gaccacgttg tgcaggagtc tcgcgcagcg ggcctgttac
     7021 cgctcggtgt agaaggtgaa aacagcgccg actggattgt cgtggatttg ggcgatgtga
     7081 ttgtccatgt catgcaggaa gagagccgtc gcctgtatga actggaaaaa ctctggagtt
     7141 aatacgtgaa gctgcaactt gtcgccgtgg gaacgaaaat gccggactgg gtacaaaccg
     7201 gttttaccga gtacctgcgt cgttttccga aagatatgcc cttcgagctg attgaaattc
     7261 cggccggaaa acgcggcaag aatgcggaca tcaagcgcat actcgacaaa gagggtgagc
     7321 agatgttggc ggccgcaggc aaaaaccgca ttgtcaccct cgatattcca ggcaagccct
     7381 gggatacgcc gcagttagcc gctgagctgg aacgctggaa gctggatggt cgcgacgtca
     7441 gtctactgat tggcgggcct gaagggttgt cgcctgcctg taaagcggcg gcggagcaga
     7501 gctggtcgct gtcggcgctt accctccccc atccgctggt tcgcgtgctg gtcgcagaga
     7561 gtctgtaccg ggcgtggagc atcaccacca accatcctta tcaccgtgag tgataaggga
     7621 gctttgagta gaaaacgcag cggatgaaac tacagaactc ttttcgcgac tatacggcag
     7681 agtccgcgct gtttgtgcgc cgggcgctgg tcgccttttt ggggattttg ctgctgaccg
     7741 gcgtgcttat cgccaacctg tataatctgc aaattgttcg ctttaccgac taccagaccc
     7801 gctctaatga aaaccgcatt aagctggtgc ctatcgcgcc cagccgcggc attatctacg
     7861 accgtaacgg tatccctctg gccctcaacc gcactatcta ccagatagaa atgatgccgg
     7921 agaaagtcga taacgtgcag caaacgctgg acgctttgcg cagcgtggta gatctgaccg
     7981 atgacgatat tgctgcattc cgaaaagagc gcgcacgttc acaccgtttc acctctattc
     8041 cggtgaaaac taacctgacc gaagtacaag tagctcgctt tgccgtcaat cagtaccgtt
     8101 ttccgggcgt cgaagttaaa ggctataaac gtcgttacta tccttacggt tcggcgttga
     8161 cccacgtcat cggctatgtg tcgaaaatca acgataaaga cgtcgaacgc ctgaataatg
     8221 acggcaaact ggccaactat gcggcaacgc atgatatcgg taagctgggc attgagcgtt
     8281 actatgaaga cgtgctgcac ggtcagaccg gttatgaaga ggttgaagtt aacaaccgtg
     8341 ggcgtgttat tcgccagtta aaagaagtac caccgcaagc cggacacgat atttacctga
     8401 cgctggatct caaactccag caatatattg aaacgctgct ggcgggtagc cgcgcagctg
     8461 tggtagtcac cgatccgcgt acaggtgggg tgctggcgct ggtttccacg cctagttatg
     8521 acccaaacct gtttgttgac ggtatctcca gcaaagatta ttccgccttg ttgaacgacc
     8581 cgaatacacc gctggtgaac cgcgccacac agggggttta tcctcccgcg tctacagtta
     8641 aaccctatgt agcggtttcg gcattgagcg ccggggtgat cacgcgcaat acgacgctgt
     8701 ttgacccagg ctggtggcaa ctgccaggtt cggaaaaacg ttatcgtgac tggaaaaaat
     8761 ggggccacgg gcgtctgaat gtcacaagat cgctggaaga atctgcggat accttcttct
     8821 atcaggtggc ctacgatatg gggatcgatc gcctctccga atggatgggt aaattcggtt
     8881 atggtcatta caccggtatc gacctggcgg aagaacgttc cggcaacatg cctacccgcg
     8941 aatggaaaca gaaacgcttt aaaaaaccgt ggtatcaggg tgacaccatt ccggttggta
     9001 tcggtcaggg ttactggaca gcgaccccaa tccagatgag taaggcactg atgatcctga
     9061 ttaatgacgg tatcgtgaag gttccgcatt tgctgatgag caccgccgaa gacggcaaac
     9121 aggtgccatg ggtacaaccg catgaaccgc ccgtcggcga tattcattcc ggttactggg
     9181 agctggcgaa agacggtatg tacggtgttg ctaaccgccc taacggtacg gcgcataaat
     9241 actttgccag cgcaccgtac aaaattgcgg cgaaatctgg taccgctcag gtcttcggtc
     9301 tgaaagcgaa cgaaacctat aatgcgcaca aaattgccga gcgtttacgt gaccacaaac
     9361 tgatgaccgc ctttgcgcca tacaacaatc cgcaagtggc tgtcgccatg attctggaga
     9421 acggcggcgc gggtccggcg gttggtacgc tgatgcgcca gatcctcgac cacattatgc
     9481 tgggtgataa caacaccgat ctgcctgcgg aaaatccagc ggttgccgca gcggaggacc
     9541 attaatcatg acggataatc cgaataaaaa aacattctgg gataaagtcc atctcgatcc
     9601 cacaatgctg ctgattttac tggcattgct ggtttacagc gccctggtta tctggagcgc
     9661 cagcggtcag gatattggca tgatggagcg taaaatcggc caaatcgcga tgggtctggt
     9721 catcatggtg gtgatggcgc aaattcctcc acgcgtttat gaaggctggg ccccctatct
     9781 ctatatcatc tgtattattt tgctggtggc ggtagatgct ttcggtgcca tctctaaagg
     9841 cgctcaacgc tggctggacc tcggtattgt tcgttttcag ccgtcggaaa ttgccaaaat
     9901 agccgtacca ctgatggttg cgcgctttat caaccgcgac gtttgcccgc catcgttgaa
     9961 gaacactggc atcgcgctgg tgctgatatt tatgcccacg ctgctggtgg ctgcacagcc
    10021 tgacctggga acatcaatcc tcgttgcgct ttccggtctg tttgtactgt tcctctctgg
    10081 ccttagctgg cgtctgattg gcgtcgcagt agtgctggta gcggcgttca ttccgattct
    10141 gtggttcttc ctgatgcatg attaccagcg ccagcgcgta atgatgctcc tggacccgga
    10201 atcagaccca ctcggcgcgg gctatcacat tattcagtct aaaattgcta ttggctccgg
    10261 cggattacgc ggcaaaggct ggctgcacgg cactcagtca cagcttgaat ttctccccga
    10321 acgccatact gactttatct tcgcggtact ggcggaagag ctgggattag tgggcattct
    10381 gattctgctc gctctctaca ttctgctgat catgcgcggg ctgtggatag ccgccagagc
    10441 gcaaaccacc tttggtcgcg tcatggctgg tggcttaatg ctgatattat tcgtttatgt
    10501 cttcgtaaat attggtatgg taagcggtat tctgccggtt gtaggggttc cgctcccact
    10561 ggtcagttat ggaggatcgg cgctaattgt gctgatggct gggttcggga ttgtaatgtc
    10621 aatccacacc cacaggaaaa tgttgtcgaa aagcgtgtaa gaggtgcgca atgcgtaagc
    10681 agtggctcgg gatctgcatc gcggcaggaa tgctcgcggc atgtacaagc gatgatggtc
    10741 agcaacagac ggtaagtgta ccgcagcctg cggtatgtaa cggccccata gttgaaatta
    10801 gcggggcgga cccgcgtttc gaaccactga acgcgacggc aaatcaggat taccagcgcg
    10861 acggtaaaag ctacaaaatc gtgcaggatc cgtctcgatt tagccaggcg ggactggcgg
    10921 caatctatga tgccgaacca ggcagtaacc tgacggcctc tggcgaagct ttcgatccga
    10981 cacagctgac ggcggcccat ccaacgcttc cgatccccag ctacgccaga atcactaacc
    11041 tggctaacgg gcgaatgatc gtggtgcgca ttaatgatcg cggcccttac ggcaacgacc
    11101 gcgttatttc gctttctcgc gcggcagctg accgtcttaa cacgtcaaac aacaccaaag
    11161 ttcgtatcga tccgattatt gtcgcccagg atggttcgct ttctggtcct ggtatggctt
    11221 gtaccacagt cgccaaacag acttacgccc tgcctgcacc tcccgattta agcggtggcg
    11281 cgggaacaag ttcagtgtct ggcccgcagg gtgacattct tccggtcagt aattcgacgc
    11341 taaaaagcga agatccgacc ggcgcgccgg taaccagcag cggtttcctc ggcgcaccaa
    11401 cgaccttagc gccaggtgta ctggaaggca gcgaaccgac gcctgcgcca cagcccgttg
    11461 ttacagctcc gtcgacaacg cctgcaacct cgcctgcaat ggtgacaccg caagccgcct
    11521 cgcaaagcgc cagcggcaac tttatggtgc aagtcggggc cgttagcgat caggctcgtg
    11581 cgcaacagta ccaacagcaa ctgggacaga agttcggcgt ccccggtcgc gtaactcaaa
    11641 atggcgcggt ctggcggatc cagcttggtc cattcgccag caaagccgaa gccagtacct
    11701 tgcagcaacg tttgcaaacc gaagcccaat tacagtcatt tattactacc gcgcagtagc
    11761 gtaaagcagg catctgatgt gtcaatttct gtaaatgacg ttaacaaagt cacgcggaaa
    11821 gtcagatgcc tgccggtagt gcatttgcta tagtagggca ctttttttaa ttccatcacg
    11881 gatgtcgtag ttcagaccat gaataccatt ttttccgctc gtatcatgaa gcgcctggcg
    11941 ctcaccacgg ctctttgcac agcctttatc tctgctgcac atgccgatga cctgaatatc
    12001 aaaactatga tcccgggtgt accgcagatc gatgcggagt cctacatcct gattgactat
    12061 aactccggca aagtgctcgc cgaacagaac gcagatgtcc gccgcgatcc tgccagcctg
    12121 accaaaatga tgaccagtta cgttatcggc caggcaatga aagccggtaa atttaaagaa
    12181 actgatttag tcactatcgg caacgacgca tgggccaccg gtaacccggt gtttaaaggt
    12241 tcttcgctga tgttcctcaa accgggcatg caggttccgg tttctcagct gatccgcggt
    12301 attaacctgc aatcgggtaa cgatgcttgt gtcgccatgg ccgattttgc cgctggtagc
    12361 caggacgctt ttgttggctt gatgaacagc tacgttaacg ccctgggcct gaaaaatacc
    12421 cacttccaga cggtacatgg tctggatgct gatggtcagt acagctccgc gcgcgatatg
    12481 gcgctgatcg gccaggcatt gatccgtgac gtaccgaatg aatactcgat ctataaagaa
    12541 aaagaattta cgtttaacgg tattcgccag ctgaaccgta acggcctgtt atgggataac
    12601 agcctgaatg tcgacggcat caaaaccgga cacactgaca aagcaggtta caaccttgtt
    12661 gcttctgcga ctgaaggcca gatgcgcttg atttctgcgg tgatgggcgg acgtactttt
    12721 aaaggccgtg aagccgaaag taaaaaactg ctgacctggg gcttccgttt cttcgaaacc
    12781 gttaacccac tgaaagtagg taaagagttc gcctctgaac cggtttggtt tggtgattct
    12841 gatcgcgctt cgttaggggt tgataaagac gtgtacctga ccattccgcg tggtcgcatg
    12901 aaagatctga aagccagcta tgtgctgaac agcagtgaat tgcatgcgcc gctgcaaaag
    12961 aatcaggtcg tcggaactat caacttccag cttgatggca aaacgatcga acaacgcccg
    13021 ctggttgtgt tgcaagaaat cccggaaggt aacttcttcg gcaaaatcat tgattacatt
    13081 aaattaatgt tccatcactg gtttggttaa aaattgaaca cttgaaagtg taatttccgt
    13141 ccccatatac taagcatcag taaaaaactc ccgccttctg gcgggagttg ctatttaatt
    13201 acgttacgcc ggagctgaca tgaaaaccaa acttaacgaa ctgcttgaat tccctactcc
    13261 ttttacttac aaagttatgg ggcaggcgtt acctgagctg gttgatcagg tggttgaagt
    13321 ggtacagcgc catgcgccag gtgactacac cccaacggta aaaccaagca gcaaaggcaa
    13381 ctaccactcg gtatctatca ctatcaacgc cactcatatc gagcaggttg aaacactgta
    13441 tgaagaactg ggcaaaatcg atattgtccg catggttctg taactcgctt ctccgttacc
    13501 cggcccctgg tcgggtaact cccctctcct cgctgtgata tacttttccc ccacttttac
    13561 tcattctcca cggagatgcc gttttgtatc aggataaaat tcttgtccgc cagctcggtc
    13621 ttcagcctta cgagccaatc tcccaggcta tgcatgaatt caccgatacc cgcgatgata
    13681 gtacccttga tgaaatctgg ctggtcgagc actatccggt attcacccaa gggcaggcag
    13741 gaaaggcgga gcacatttta atgcccggtg atattccggt gattcagagc gatcgcggtg
    13801 ggcaggtgac ttatcacggg ccggggcaac aggtgatgta tgtgttgctt aacctgaaac
    13861 gccgtaaact cggtgtgcgt gaactagtga ccttgcttga gcaaacagtg gtgaataccc
    13921 tggctgaact gggtatagaa gcgcatcctc gggctgacgc gcctggtgtc tatgtagggg
    13981 aaaagaaaat ttgctctctg ggtttacgaa ttcgacgcgg ttgttcattc cacggtctgg
    14041 cattaaacgt caatatggat ctttcaccat ttttacgtat taatccttgt gggtatgccg
    14101 gaatggaaat ggctaaaata tcacaatgga aacccgaagc gacgactaat aatattgcac
    14161 cacgtttact ggaaaatatt ttagcgctac taaacaatcc tgacttcgaa tatattaccg
    14221 cttaatttca tacctcaatg gcccaattta cactgggtca ttactcataa cacctgctaa
    14281 tgctttacat ctccttcgcg tttgtatatt ttctttatca cttgcagaaa tgcgatgcta
    14341 cgccttgctc cgtaactcac cccattgatt aacttattga aatagttgat tatgatgagc
    14401 aaaagcgttc cgttccaatt agacataaga ttattgtaat ttttttaaat attatttttc
    14461 catgaataaa attggagtaa gtgcgtggat agtaataatc aaattgaatc ctgtttaagc
    14521 agaaaatcgt cagaaggtaa accacaaata tttacaacct tacgaaatat tgatcttaat
    14581 cttctgacta tttttgaagc tgtatatgta cataaaggga tcgttaatgc agcgaaagtg
    14641 cttaatttga ccccctcggc aatcagtcag tctattcaga aactgcgcgt tatattccct
    14701 gacccattgt ttattcgcaa aggccagggt gtcactccta ccgcatttgc gatgcatcta
    14761 catgagtata tcagtcaggg ccttgagtcc attcttggcg cgctggatat cgaaggaagc
    14821 tatgataagc aacgaacgat aactattgcg accaccccct cggtcggagc cctggtcctt
    14881 cctgtcattt atcgggcgat taaaactcac tatccgcagc ttttactgcg caacccgccc
    14941 atcagcgacg cggaaaacca actcagtcag tttcaaaccg atctcatcat cgataacatg
    15001 ttttgcacca atcgtacggt gcaacatcat gttctgttca ccgacaatat ggtgttaatt
    15061 tgccgcgagg gaaatccact actctcttta gaagatgaca gagagactat cgacaacgct
    15121 gcacatgtac tcctgttacc ggaaggacaa aatttcagcg gtctgcggca gagaattcaa
    15181 gagatgtttc cggaccggca gatcagcttc accagctaca acattttgac aatcgctgca
    15241 ctggttgcca acagtgacat gttagcgatt attcccagcc gtttttataa cctgtttagc
    15301 cgttgctggc cgctggaaaa actgcctttt ccgtccttaa atgaggagca aatagatttc
    15361 tctatccact acaacaaatt cagcctgcgt gatcccatcc tgcacggagt aatagatgtt
    15421 atccgtaatg cattttgaaa aagtattgtt acagccagat cacagcgcag aacgataaaa
    15481 tggcacaatc caacaacaat tttacatttt cgcgaccgct ttggctgctt tcaggtccgt
    15541 ttcaatgata tactgccagt cgttaattca aaaatagttg ataattacaa caatctattg
    15601 aattgaaacg ctttccttcg taattcgcaa ctggaacacg cacgctatga gtaaacccat
    15661 tgtgatggaa cgcggtgtaa aataccgcga tgccgataag atggccctta tcccggttaa
    15721 aaacgtggca acagagcgcg aagccctgct gcgcaagccg gaatggatga aaatcaagct
    15781 tccagcggac tctacacgta tccagggcat caaagccgca atgcgcaaaa atggcctgca
    15841 ttctgtctgc gaggaagcct cctgccctaa cctggcggaa tgcttcaacc acggcacagc
    15901 aacgtttatg atcctcggcg ctatttgtac ccgccgttgt ccgttctgtg acgttgccca
    15961 cggtcgcccg gtagctcctg atgccaatga accagtgaaa ctggcgcaga ccattgccga
    16021 tatggcgctg cgttatgtgg ttatcacctc cgttgaccgt gatgacctgc gcgatggcgg
    16081 tgcccagcac tttgcggatt gcattactgc cattcgggaa aaaagcccgc aaatcaaaat
    16141 tgaaactctg gtgccggatt tccgcggtcg tatggatcgt gctctggata ttctgactgc
    16201 aacgccacca gatgtgttca accataacct ggaaaacgta ccgcgtattt accgtcaggt
    16261 acggcctggt gcagattaca actggtcgct gaagctgctg gaacgcttta aagaagcgca
    16321 tccggaaatc ccgaccaagt ctggtctgat ggtgggtctg ggtgaaacca atgaagaaat
    16381 tattgaggta atgcgcgacc tgcgccgtca tggtgtgacg atgttaacgc tggggcaata
    16441 tttgcagcca agccgccatc acctgccggt tcaacgttac gttagcccgg atgagttcga
    16501 cgaaatgaaa gccgaagcgc tggcgatggg ctttacccat gctgcatgcg gtccgtttgt
    16561 ccgctcttct taccacgccg atttgcaggc gaaagggatg gaagttaagt aaacctgtaa
    16621 taattactta catctgtctg ataaaaaacc cgctttttga agcgggtttt ttgtatcgaa
    16681 caagatattg agggagcgtc ctgctcgcca cgtcactctt tatgagagag cttttcagcc
    16741 tgaagatcaa cgtctgcgcc ttttttcgcc gcagcatcgt catcattcat cgccttcttg
    16801 aaccctttaa tggccgctcc aaggtctccg cccagcgtac gtaacttctt agtcccaaac
    16861 agcagaacga ccagcgccgc aactaccagc agtttggtaa tactaatctc acccatagat
    16921 accttcttga catataaccg gcaacagaac gcgccttaaa aaccagagaa tattaacggt
    16981 cattcgacgc gcgcacacaa tagcaatctg taacaaggtg aatcaagcgc cgagtaaaaa
    17041 aacatcataa taattgcggc gacgcaaagc gacggttgtt taagacgggc agttgcgcgc
    17101 gcacctgacg cacacgttcg ggcgtcactt ccgccataat gagtgcaggc atttctgacg
    17161 ctgccgcaat ggtgacgcca aagggatcta taatccggct ttgcccgata tttttgttcc
    17221 cgcactcccc cgccgccacc atataacagg tggtatccag cgcacgagcg gcaagcaacg
    17281 ttgaccagtg atgctctttg agcggcccgc gaacccaggc ggcaggaagc accaggattt
    17341 cagctccctg taatgcctgt gccagcgcca gctctggaaa gcgtaagtca taacaggtca
    17401 tcagaccgac cttcatccct tccacctcca gtaacggagc tatttcatta ccagcatcaa
    17461 catggcgtga ttcctgaatg gcaaatgcat catagagatg cagtttggca taacgggcga
    17521 caatgtttcc tgcctgtagc gccaccagca tattccatgc gcgccccggc gttgaaggaa
    17581 catgaatcgt cagaattgtc gtcatcatgt tacgtttact ttctcgccgt aaacgtccga
    17641 ggaattcgcc ttccagcagc tgtgctgatt taaccgatag atctgcatca tgatcatcgc
    17701 gtgccagcaa tgcttccggc aggacaaaca gcgatacgtc gttttccgcc gcctgcgcca
    17761 tcaacgaggc acaaatctca gcgttctttt cccacacaga tgtaacagca aactgtcctg
    17821 cagcaaccag cattgccacc tcctgtcaga gtcgtaaaat tcgccaacga cgattattcg
    17881 gcgttacact tatcactcat acaaatcaaa tagcaggatt ttgcagtgtt acaacttctt
    17941 ttagcagttt ttattggcgg tggtacagga agcgtggcga gatggctgtt aagtatgcga
    18001 tttaacccgc tgcatcaggc gattccgttg gggacgctgg cagcaaacct gattggggca
    18061 ttcatcatag gaatgggatt cgcctggttc agcaggatga cgaacattga tccagtgtgg
    18121 aaagtattaa tcaccaccgg attttgtggc ggtctaacaa ccttctcaac attttcggca
    18181 gaagtggtgt ttttgttaca agagggccgc tttggctggg cgttactgaa cgttttcgtc
    18241 aaccttctgg ggtcttttgc catgaccgca ctggccttct ggctgttttc ggcctcaacc
    18301 gcacactaaa gacaaaaaaa acccgctgat taagcgggtt ttgaattctt gctgacgtat
    18361 cttacagagc gattacgttt gcagcagaag ggcctttggc accgttagtg atttcgaact
    18421 ctacgcgctg accttcagca agagttttaa aaccattagt ctggattgca gagaagtgta
    18481 cgaacacgtc tttgctgccg tcttccggag taatgaaacc gaatcctttg gactcattaa
    18541 accacttaac gttaccttta atcttagaca tcaaaattac ctttacatga aaaatagaca
    18601 caaatgctgt gtcggttacc agtacaccaa ttgtggtacg ctttgtccag tgcaacttta
    18661 ctaaaaagtg ataaaagtcg cgttattttt gatgttaatt attttactca ttgattttaa
    18721 agaatttact aaaacttcat ttgtctcaaa actgaaagcg catccaggca aagtacacat
    18781 tgccattgtt gtaggtaccc ggaatatagg tcatctgaaa agtcactgga ccataaccca
    18841 cggaggccaa tggcagtaga accgggagag ggatgtaatt ccagttatca cgtgccgtta
    18901 cgccagcggt gaatcccaga cctaaatgaa aattttcatc cgccagcggt cgccaggtac
    18961 tttcccatcc gtatccggca atcggttccc atttgttcca cgagtcctta aatgccatgg
    19021 catacaggcc atgccagttt cctttttcat cccaacgcga caggccaaaa ccgccacccc
    19081 acggtcgctc gttatagcga tcggtttttt ctttgtcgta agcgaaacgt gcatgccagg
    19141 tgatggctgg aatatataaa tcataatgtt caggctgttg ccaggtttgt gcaatatttt
    19201 ctctaaacgt tgtcatccac tcatctgcgt tagcaaaaac tttaccaacg ctgattaact
    19261 gaataaaaac aaaggaaaag atagcgacat atttactcac gttcatttgt gaccataaaa
    19321 catttatcaa aaatctacta ctagcatagc aaagctacaa ttaacataac cttaatagcc
    19381 ccaacataaa gaataatctg aatagctgct ctatttattt ccgcttgttc aagttaaata
    19441 tttccgcgca tcgatttaag atgatacttc gttttctgtg atgggaatag tcatttaata
    19501 aaaagcaagt cgtttctgat aaca
//
LOCUS       DAPGOF010000089        19431 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.089, whole genome shotgun sequence.
ACCESSION   DAPGOF010000089 DAPGOF010000000
VERSION     DAPGOF010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.089"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            65..829
                     /locus_tag="RHV13_002754"
     CDS             65..829
                     /locus_tag="RHV13_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242275.1"
                     /translation="MYKLVSPRELTEIITGLLLKPELLGELDSPEKHRMFMADLGRVV
                     AEHCGGLVNSVVLPGAEVTVPDGLPLNGQPVRYLGIKESIPHLLVEPDASLPSLAKNV
                     WMYADPEGWKEHFGAEEDCLTHEMMQLFRKGIQALQKEHDTPEISLTLQDWRLEEETL
                     REEDSQAYQVSVTGQHNIHCEVVNKEGNPCFGVMFEIDQGVPALHINTGGDMLLHIHC
                     AHNGLVLTPDASGQRFEKAPVDRFAYNSPSLLLSAD"
     gene            910..1671
                     /locus_tag="RHV13_002755"
     CDS             910..1671
                     /locus_tag="RHV13_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HDV1242276.1"
                     /translation="MQISTEVLNVLSRCRAEGNFLFLADQLDRSIYVKTNKVLEAAGG
                     KWNRKEQAHIFTADAAERIEQIILTGSVDIPRDLFNFFPTPENLVTDMVLRAEPAAGE
                     RVLEPEFGDGRILKALKLAAPDALITGIELNDERFLAVKNDSVLSTGAELVHTDFLGY
                     QPDETFDVIVMNPPFLKRSDVKHVMHAIAMLAKRGRLQAILSAGVLFREDTLTKALRE
                     RVKQLGGQISPLPDDTFRESGTKVKTARLEIDLRR"
     gene            1765..2028
                     /locus_tag="RHV13_002756"
     CDS             1765..2028
                     /locus_tag="RHV13_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242277.1"
                     /translation="MTHTAVSQANSALQLPTLEHVYALLKANCKPDRFDGRDGPVWGQ
                     EYSWNLAKDRLQDLEKYGKAYVSRHEDRMGEGFSFGPDLLIIR"
     gene            2073..2660
                     /locus_tag="RHV13_002757"
     CDS             2073..2660
                     /locus_tag="RHV13_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242278.1"
                     /translation="MFSKPMTTFTPERIEQILEFSEGSNPSEAMSLRADEVAVLARIG
                     KAVMSIAPVYQCEFCHHDATGQLQWHWEDVNKAFYDKYDPDRRGRRRILYTAPPMPLV
                     SADLLNMASSAIEDLLTNKDRSGAGMWNDIPEQLRRAAELVMDNSRAAEIEAMLMRLY
                     VECDTGERRGNGSQSGVAMPSWQTVEEARVLLGVK"
     gene            3125..5545
                     /locus_tag="RHV13_002758"
     CDS             3125..5545
                     /locus_tag="RHV13_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4165 domain-containing protein"
                     /protein_id="HDV1242279.1"
                     /translation="MKHLAFITAVAGLGMSVQAPAQIYESAFKDTNGIEIHAPSSRLM
                     LNPASPVTLTLISGLDRFVNVKVTKDTGTVILNTTTTRTGVSDRLTAADGSEFYGKKV
                     TLPALGEGKFVVQINVLDLNQKPVATYNYNWLIDVTPPAANALTANTGSGSTAGDVWK
                     LGLEATGQYDFTSSGVSDANGIDKGLIYIYRQDGSLYSTTQMQYDVSGQKMYHTYSKN
                     SVKGTGIPDSNLDEDFTAKVVIFDNAGNSRTLPTQKFRYDNTLGEMTLWAVHDPNTSS
                     SVVPGVSNYPAYKAGMVVNENPIRLVYRIPKSNYRAYSEGGLQFINQYSAPKEIAVDS
                     TYAYVEMTLPYGSINGDMARMANFGQWGGYYPSYSLVLNPSANQTPAFAGTWVDFLDD
                     KGNWVKWKDFESVASSRLPIKISRLRFNVEARPFAQEIGGKATCTIPAGKTSCEAPET
                     FDMALGTQGYNRILYFVRSISNPILRSEQWIMTRWNNKQLPVINSISYDETNKQLDVL
                     ASLEGDGNWFDSVSLREFYLSDKNTGTRMSPTGVIKSRISGNYTIAYDLSRQSEGKYN
                     VEVNIRDFFQNQTNKTFGEIALDNTPPTVAITFDGKPVKDDTVVYGLENLRIALADNL
                     TTPRITRLQLVGGPTADNVELTWSPAGKDTYMPEYPRLFPNFEPSENYSISVTVADSQ
                     SNTKTYTQKFSYLPNNLVQLHNLRTLSVSSPLKTTDGVPLAYLSTNVLRKTNGEIAKG
                     VQNATLTVRKDAAFGIKFNGAQAAPGESVEVQIDMGQGDNLLLPVYPSENGKVGTSEF
                     MIQIDELK"
     gene            complement(7624..8712)
                     /gene="repHI2"
                     /locus_tag="RHV13_002759"
     CDS             complement(7624..8712)
                     /gene="repHI2"
                     /locus_tag="RHV13_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncHI-type plasmid replication initiator protein
                     RepHI2"
                     /protein_id="HDV1242280.1"
                     /translation="MTKEKDTEQQDLVTRAFSVREKESGKDIILRPNSNRTVQSIALM
                     RLGLFVPSPKSVGRQNREYKTVGFDATKELQTLSLMESEGFTNISIVGERLNMSVDFK
                     TWMGIIRTYANHPINNDTISLKFTEFLKLCTPENYRSSTASRKRIDASLRRLASVTLS
                     FTSNNSSKVYTTHLVQSALLDPESDQVVLQVDPKIFELYQYDHKVLMQLKAIKELAKK
                     ESAQALYTFIESLPPNPIPISLTRLKNRLNLKTRANSQNATVRKALEELASIGYLKYT
                     EIKKDGKVYFQIHKRDPDLNLNNTQPPLEVVEDEEENSGSSVLEGELCPPADPIDGDD
                     VLTVHDLTAEELRYIRSLRSQKKNSNAS"
     gene            complement(9820..12276)
                     /locus_tag="RHV13_002760"
     CDS             complement(9820..12276)
                     /locus_tag="RHV13_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4165 domain-containing protein"
                     /protein_id="HDV1242281.1"
                     /translation="MRKTLIGCMVATALATVSSAHAQVFSYSFTDTNKAVRNIKPATQ
                     TYLNPAGVLTLNLISGLDRYERVTVTRDSDKKVMYSSVSTKTSVADRIVAADGTEYYG
                     KDMVLPALGEGTFTVVNETLDIRQTVVSTSTYHFIVDTTPPRYKSIYPSQNAGYDMVL
                     SGPLWELGRGGSGQFSIFADGIEDASGIDKIRLVIKRSNGSVVSDNNLSYDTANKRAF
                     YPWIKDMNTQAGMPSSDLDEEFTFNFIVTDLAGNTLNIPPQRFLYDDQMGEFTPFAVH
                     DSRVSTSVVPGISSGYVPFKRGLTVLENPYKLVIRIPRTNWKPYRNGGMSITNNYGGV
                     QVLSEDATYVYVEVKLPQGALDGNNYRPVNTYQWSGGDLGQYASWLNWDPASVKSPAW
                     GSSPIERQKADGTWFNSVNWNLFKASDMPIKLTNIRFNVQARPYDQKITGGATCSIPA
                     GSTTCTVSLPQDIINGTTGYLHSGYEVRSTTEATFFAPIWENIAWHTLGPSVTGFDYN
                     ETTNILQVYVNQPGDGSYFDHVYVNRVWLSDKNRNNAEISVTGKQTGRNMASGNYTYE
                     FNMKEVPEGSYNVVINAQDTFNNTGNLPYQTVVVDNTAPSVSISYEGKPISKNVTVYG
                     LENVRIQLTDALTKPSLSRMTLRGGPVSDAVELSWVNLGNNLYAPNYPKIFPSLNEGE
                     TYTLTVQAKDEMNNVKESSVEFNYLPNNLVRLENLKTLAVNASLKTSDNTALAVLYAS
                     QLRKKDGSIATGLQDAVLTVRKDAAFGVTVNGVSAMPGESKELQLDLGLGDSRSFPIF
                     PAVSGLTGRSEFMINIEELK"
     gene            12649..13275
                     /locus_tag="RHV13_002761"
     CDS             12649..13275
                     /locus_tag="RHV13_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000966518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242282.1"
                     /translation="MLYMLLCCFLMLNSTFVMFRAMSAISKGSAKENRSEISLIVLAT
                     LGIASPFIVAMITINESMTSKTVTDFSLGAQWYGMVSAVALMGLYARRVWKEKKSLFT
                     GAFLASSLMAFIFTDSLVFVSQKDTGVLATFVLDKNAGDIDCSRPAMIVHYSKGVPTD
                     WRCPTSIMLMAYSSYPFLPWPEYSHGTSQSLTVVIDTFMENAVNLSQK"
     gene            complement(13333..13614)
                     /locus_tag="RHV13_002762"
     CDS             complement(13333..13614)
                     /locus_tag="RHV13_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000535423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242283.1"
                     /translation="MGSPKFEIAEQIKMWIVVYLVCFLALYCVLSALHALYDPLNLPA
                     LTWPISWFLVPEMTSFGSALLAFVLTPGLCSIIAVLFGKSIFKKAKTKI"
     gene            complement(13595..14056)
                     /locus_tag="RHV13_002763"
     CDS             complement(13595..14056)
                     /locus_tag="RHV13_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242284.1"
                     /translation="MGDIVIKRRNIRRSVYGLLIVLIAGNVWLGLRADKIHKVRYQDF
                     WSPATVIKVTVMPSTNETQLSGKIPKSVRVSNNYVEYSLPGTLSAKTIYRSVLEDEML
                     TLLNAGGQLEVKYTLDKQTNRTKVCTKCLRVIKDINHQYSATEVKHGQSKI"
     gene            complement(14105..14281)
                     /locus_tag="RHV13_002764"
     CDS             complement(14105..14281)
                     /locus_tag="RHV13_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242285.1"
                     /translation="MSNQPTSLPEEDIEFVMDTFQRSMGKSKPVRDTQLEGEKPQSKE
                     SANSTENPGYEEFY"
     gene            complement(14278..15969)
                     /locus_tag="RHV13_002765"
     CDS             complement(14278..15969)
                     /locus_tag="RHV13_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HDV1242286.1"
                     /translation="MSSLDSEAKPDNAGHSVLALTTSHSLVVSSPETFLPDMRKELGI
                     IADLVESYNDELCLLKLMAVQFKTHNHQKLYSYLSGYNHSISEADALFAENALRSEYW
                     KRVMALTDVLPIMSDAKRNEWDKQFTADRYIMPPQVIPDFTADAVVGTVVALLNDRNQ
                     FIKERVYDVFQSLSRSHKTNKAFGFSTRMIPTGVCEPSKYPWQKLRVDFKESGISPLS
                     ELRVICAFFRGEQVKAIHNTKSLVEALVEHEGFRKWICIDGNSIRFRVYKNGSMHIDV
                     HPDIAERLNNILSAIVPLALPADRMAHSKKSLEAFPVLKQCIDFDTRMQLSELMFKND
                     GDNKWSCWTSLGSLAERKSSSVAADTLRFLGATVTKYDVTFSYDPCEVIRYIGQIGEM
                     PDIVSHQFYPSSCRISEYVSSLLGAGEGDTLLEPNIGHADLLKSFPAGVIVTGIELDT
                     LNCLISRAKGYDTTEADFLTWSKSNQQKKFDYVVMNPPFADNRARLHLQAAASHLVAG
                     GSLAAVLPLSLQGLDNLLGEEFRTEWMDVFENEFENTTVSVRILYAERIQQEEVL"
     gene            complement(16217..17380)
                     /locus_tag="RHV13_002766"
     CDS             complement(16217..17380)
                     /locus_tag="RHV13_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDV1242287.1"
                     /translation="MSVLLTEPTQQANDKVFKTAHVAFSVVTGTGRYVTGLKQFRDAN
                     PELCTEVSDQKAWAIHPSVIQVTPGFNSREMGMGDDYYKLPEVEEHIYNIKNAYIRGD
                     YVDPIRVRVIDGVPFVRQGHCRLKAAMMACDEDHDITILCVEIKEDEIGCELATIDGN
                     RGLALSPVALGESYRRLHSLAGWSLERIAQRENKSPTTISSLIRLTTCSVVIKKWIHA
                     DAISYVNVLSLIDELGETEAISRIKKMIAELEQADANGITVKKTQHGQVRVRPSDFKP
                     ARIPPVIATKAVEGVKLITTSLLQKLGDIELPELTDSSADEEINITLNRSTLEMLRNL
                     SKEITESENKQLRRAENRQAKLNGEKPKYPRKKNAKKAGEETDQDTDPQPDAE"
     gene            17750..18562
                     /locus_tag="RHV13_002767"
     CDS             17750..18562
                     /locus_tag="RHV13_002767"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HDV1242288.1"
                     /translation="MPIIKWAGGKTKLMPFISHHYPHDHSCRWVEPFIGGGAVFLNMF
                     AQNALLADSNPDLINLYRTIQRQKTNFINQVQNLADKTFVEKDYYEMRDRFNKTCISG
                     QSLQRAALFYSLNRLGYNGMCRYNSERIYSVPWGKHTELKLDLNKIDYLSFRLSGIEL
                     ITAGFEETLAATGEGDQIYCDPPYDKTSKTSFVSYDGKPFSQSDHVLLANMLVDAHRK
                     GAAVAISNSLTSFTLGLYEERGFVIHRLSAYRSVGSKPNTRKTETEILAVLK"
     gene            complement(18571..19425)
                     /locus_tag="RHV13_002768"
     CDS             complement(18571..19425)
                     /locus_tag="RHV13_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000970401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242289.1"
                     /translation="MMEMDFKMHNDLGLGFICKNARTSSKPTLRKVTFPVSAYETSKL
                     SLTGFQCPTACRFPFFVLCMIIHNHLSSACPITQNECSNHISQFSIDIKVQDWLSRSR
                     VAFIDFHNLRNTDKTTLITVNHLEALLTVMSTTLVAYVPYSKKRLNFSFLNSFTLSKT
                     SQSYTLTFPVVLSPLLDALGDFIQECITEKLLKRRNSNFMVYEYLKRSGQSSHKVEDI
                     NNDLQLKSLNIRLMSVLTGLSQQGLISFICEGKRGDRRIEELQFIPYVQRTYPEVLTF
                     EEWISLVD"
BASE COUNT         5407 a         4344 c         4372 g         5308 t
ORIGIN      
        1 tggaggttat tagctcgtgc atctggattg ttaatataaa gccaacacag aaaaggaaaa
       61 caaaatgtat aaattagtat ctcctcgtga actgactgag attattaccg gtttactgct
      121 taaacctgaa ctccttggtg agctggactc accagaaaag catcggatgt ttatggcaga
      181 tttaggccgg gtggtggcag aacattgtgg aggtctggta aacagcgtcg tcttgccagg
      241 agctgaagtt acagtgccgg atgggttacc gcttaacggc cagccagtac gttatctcgg
      301 aatcaaagag agcatccctc acctccttgt agagcctgat gcttccttac cttcgctcgc
      361 taaaaacgtc tggatgtatg cagatcctga aggctggaaa gaacattttg gagctgagga
      421 agactgcctt actcatgaaa tgatgcagct gttccgtaaa ggtatccagg ctcttcagaa
      481 ggagcatgat acccctgaaa ttagcctcac acttcaggac tggcgcctgg aggaggaaac
      541 actgagagaa gaggacagtc aggcctatca ggtcagcgtt accggccaac ataacatcca
      601 ctgtgaagtt gttaacaagg aaggtaaccc ctgttttggt gtcatgttcg aaatcgatca
      661 gggtgtgcct gccttacaca tcaacaccgg cggggatatg ttgctccaca ttcattgcgc
      721 ccataacgga ctcgttctga cccctgatgc aagcggtcag cgatttgaga aggctcccgt
      781 tgaccgcttc gcttataaca gcccatccct gctgttgtct gccgactaac caaggccaaa
      841 tctgaattgt cctgtttaag taaccaaccc gctgccgcca ggcgggtaaa tcctcttaat
      901 ggagttttaa tgcaaatctc aactgaagtt ctgaacgtct tatctcgctg ccgtgctgaa
      961 gggaactttc ttttcctggc cgaccagctc gaccgcagca tttatgttaa gacaaacaaa
     1021 gtgctggaag ctgccggtgg caaatggaac cgaaaagagc aggcacatat ttttacggcg
     1081 gatgcagctg agcgtatcga gcaaatcatt ctgacaggtt cggtcgacat cccacgtgat
     1141 ttgttcaatt tcttccctac ccctgagaat ttagtcacag atatggttct acgcgctgaa
     1201 ccagctgccg gggaacgtgt tctggagcct gagtttggtg atggccgcat tctgaaagcc
     1261 ttaaaactgg cggctccgga tgcattgatt accgggatcg aattgaacga tgagcgattc
     1321 cttgcagtga agaacgatag tgtccttagc acaggggccg agctggtgca taccgacttt
     1381 cttgggtacc agcccgatga aacttttgat gttatcgtta tgaatccacc attccttaaa
     1441 cgctctgacg ttaaacatgt catgcatgcc atcgcaatgc tcgctaagcg aggtcgctta
     1501 caggccattc tgtcagctgg ggtattgttc cgggaagaca cactgacaaa agcgctcagg
     1561 gagcgagtca aacaacttgg cggacaaatt tcgcctcttc cggatgatac atttagggag
     1621 tccggaacaa aggtcaaaac agcccgcctt gaaattgatc ttcgccgcta actgacaagc
     1681 aaccggaaag aaatcttaat acgagtagcc ggttaaagtt acctgaccgg cttaactgac
     1741 taaatcaata aagagtgaac agaaatgacg catactgccg tttctcaggc taatagtgct
     1801 ttgcagctac ccacgctgga gcatgtctac gcacttctga aagcaaattg taaacctgac
     1861 cgctttgacg ggcgtgacgg acccgtgtgg ggccaggaat actcgtggaa tctggcaaaa
     1921 gatcgcttac aggatctgga gaaatacggt aaggcatatg tctcccgtca tgaagaccgt
     1981 atgggggaag gatttagttt tggtcctgac ctgttaatta ttcgctaacg ctcttaaggc
     2041 ctgatgatat ggtaagcaca actggaaaaa atatgttttc aaagcctatg accactttca
     2101 caccagaacg tattgaacaa attttggaat tttccgaggg tagtaaccca agcgaagcaa
     2161 tgagtcttag agctgacgaa gttgctgtac tggcacgtat tggaaaggca gttatgtcga
     2221 ttgcgcctgt ttatcagtgc gagttttgcc accatgacgc aaccgggcaa cttcagtggc
     2281 actgggaaga tgtgaacaaa gctttttatg ataaatacga tccagacaga cgcggaagac
     2341 gtcgaatcct ctataccgcc ccgccgatgc ctttagtttc ggctgacctg cttaacatgg
     2401 cttcatctgc tattgaagac ttgctcacta acaaagacag aagtggtgcg ggaatgtgga
     2461 atgacattcc agaacagcta cgacgcgcag cagaactggt aatggataat agccgggccg
     2521 ctgaaataga agcgatgctg atgcgtcttt atgtagaatg tgacaccggt gagcgcagag
     2581 gcaacggcag tcaatctggc gtagcaatgc cttcgtggca aaccgttgaa gaggcacgtg
     2641 tgttgctggg ggtgaaatga tgcagttctc gccagggcag tttgtaattc acaacaagaa
     2701 gcgccttgcg gaacgaaggt agacgtactg actgaggttc actcccctct cgactggtgg
     2761 cgtaataccg ccaggttttg attcatatat gccgtccgtt aacgcattta tatctgtcat
     2821 acctaattta tcctgattct tttttacaca aagtaaaagc atcacttaat gcgatatgtg
     2881 gatgccgata gtttcaggct aatgttataa gttttgcaat cgttgccagt ttggatacca
     2941 cagcaatgtc agatgtaatt ccaaaaagaa agatttacgt cctgaacgca caataaggtt
     3001 tgtcgaaagg caaattatag atttgtaatt tcgagcacca tatggagaag ttttttatac
     3061 ttttacgaac atagtgacct ttaggaaaag cttaaaataa caccaaacct aaaggggaat
     3121 aagaatgaaa catctcgctt ttattactgc tgtagccgga ctcggtatgt ctgttcaggc
     3181 cccagctcag atatatgaat cggcctttaa ggacacgaac ggtattgaga tccacgcccc
     3241 gtcttctcgt cttatgctta atccggcatc accggtaact ttgacactta tttcaggtct
     3301 tgatcgtttc gttaatgtca aagtcacgaa agacactgga actgtcattc ttaatactac
     3361 gactacacgg acgggtgtat cagaccgact aacagctgct gacggtagtg agttctacgg
     3421 caaaaaagta actttgcctg ctttgggtga aggcaaattt gtcgttcaga taaacgtgtt
     3481 agatctcaat cagaagcctg tagcgaccta taactataac tggctaattg atgtcacccc
     3541 tccagcggca aatgctctta ccgctaatac tggttctggc tctaccgctg gtgacgtgtg
     3601 gaagcttgga ttagaggcaa cggggcagta tgacttcacc tcttcgggcg taagtgatgc
     3661 aaatggtatt gataagggcc taatatatat ttacaggcag gacggtagcc tctacagcac
     3721 tacacagatg cagtatgacg tatccggcca aaagatgtac cacacttact ctaagaattc
     3781 agttaaggga accggaatac cagacagcaa cctggatgaa gactttactg caaaagttgt
     3841 tatcttcgat aacgcaggta atagccgaac gctgccaact caaaaatttc gctacgacaa
     3901 cacgctgggt gagatgacac tgtgggccgt tcatgatcca aatacgtctt ccagcgtcgt
     3961 acccggggtt tctaattatc cggcttacaa agccggtatg gtcgttaacg aaaaccctat
     4021 tcgattagtc taccggatcc caaaatctaa ctaccgtgct tattcagaag gtgggcttca
     4081 gttcatcaat caatattccg cccccaaaga gatagctgta gacagcactt atgcttatgt
     4141 tgaaatgact cttccctatg gctcaattaa tggggatatg gctcgtatgg cgaactttgg
     4201 ccagtgggga gggtattatc cgtcatacag cctcgttcta aacccatctg caaaccaaac
     4261 gcctgcattt gcgggtacct gggtagattt cctcgatgat aaggggaact gggttaagtg
     4321 gaaggatttt gagagtgtgg cttcatcacg actgccaatt aaaatttccc gacttcgttt
     4381 taacgttgaa gcccggccct ttgcacagga gatcggcggt aaggcgacct gcaccattcc
     4441 ggcaggaaaa acctcgtgtg aagcgcctga gacgtttgat atggccttgg gtacccaggg
     4501 ctacaataga atcctttact tcgttcgcag catcagcaat cccattttgc ggtctgagca
     4561 atggattatg acacgctgga acaataaaca actgccggta ataaactcga tatcatatga
     4621 cgagactaac aagcagctgg atgtactggc gtcacttgaa ggcgatggta actggttcga
     4681 ctcggtctca ttgagggaat tttatctttc cgataagaac accggtaccc ggatgtcacc
     4741 aacaggcgta atcaaatctc gtatctcagg taactacacg attgcttatg atttatcccg
     4801 tcagtctgaa ggaaaataca acgttgaggt caacatcagg gacttcttcc agaaccagac
     4861 caataaaact ttcggagaaa ttgctctgga taacactcct ccgacagtgg ccataacatt
     4921 cgacggaaag ccggtaaaag acgatacggt agtgtacggc ctggagaacc tcaggatcgc
     4981 tctggcagat aatctgacaa ccccgaggat aacccgtctt cagctcgtag gtggccccac
     5041 agctgataac gttgagctta cgtggtcacc ggcaggcaaa gatacataca tgcctgagta
     5101 tccgaggctg ttcccaaatt tcgaaccatc tgaaaattat tcaattagcg taacagttgc
     5161 cgatagtcag tcgaatacca aaacctatac tcagaagttc agttatctac cgaataacct
     5221 tgtgcagtta cataatctac gcacattgtc ggtcagttca cctctcaaaa cgacagatgg
     5281 cgtaccactt gcgtacctgt ccactaacgt tctgcgtaag acaaatggag aaattgctaa
     5341 aggggtccag aacgcaacgc tgactgtgcg gaaggatgca gccttcggta ttaaatttaa
     5401 tggggcacag gcggctccag gcgagtcagt tgaggtgcaa atagatatgg gccaggggga
     5461 taatcttctg ttacccgttt atccttccga aaatgggaaa gttggcacct cagaattcat
     5521 gattcagatc gacgagttga agtaactacc cggagccgac agtaaaactg acggcttcaa
     5581 ttttactgcc ctcctcgact ccgatcagcc agtacgtctt cagagattcg caaggaagaa
     5641 ccgaaagaaa agttacttta atcgtacgtt tccagcgatg ctttcaaaag acaatgcata
     5701 ctcgttgatt acgacaatgg gagtgagtgt cgaacccctt gagtaaggat acctggtaaa
     5761 tatacagccc cggggattct cttccttcaa ccggggatga caacgggagt gagtccggca
     5821 actcacacct gttgtcactt ctctgacata tcgtcatacg agcttactca agcgcatcca
     5881 caaagatggg atcgttttga atctaaactg gtactggtta gccagtcact tgcatctact
     5941 cccccgtcga tatttaagtg acaattgtag tgatctgcaa tactggtgtc tcagaaacac
     6001 tgacgacggt agagactcaa taccctaacc cctgacaaca gaagtgagct tagaggtgtt
     6061 acgctgctgt cacctaatgc ttgggaattg cctgactcac aaccattgtc atccggagct
     6121 actgattaac gttgaaccgc tggactttaa tgacagtggt agtgatggat aggctcattt
     6181 ccattgtcgt cattttatcg gacaagcctc ttcgtcctac aggtttaagc tgacaatggt
     6241 agtgattgga tgatctcact actgttgtca ctcagttctt tgagcggacc cttccctcgg
     6301 ttggtcggca ttaacaacgg tagtgactcg attggctcac tgccattgtc atcgtgtaaa
     6361 tgacgtacct tatcagctca gctgaaagca atgacaacgg tagtgactct aattgctcac
     6421 tgccattgtc gtcgtgtcta ttgagagcct tatcagtgca cctgaaaagc aatgacaacg
     6481 gtagtgattc taatggctca ctaccgttgt caccgtgtgt attgagagga tggtccactt
     6541 aactggacgt atatgacaat ggtagtgact ttaatagctc actaccattg tcggtgtgtg
     6601 aattgagagg atgagcagct cagctgtacg tccatgacaa tggtaatgac ttaatgagct
     6661 cactaccgtt gtcaccctgt taattaagag gctcatctgc tcttctggaa ggctatgaca
     6721 acggaagtga cttgctgggc tcactgccat tgtcgccgta tgaattgaga ggatcatccg
     6781 ctcttctgga tggcaatgac aacggtaatg acttaatagg ctcactacca ttgtcaccct
     6841 gtgaattgag aggatgatct gcttaaccag acggccatga caacggtagt gactttctgg
     6901 gctcaatacc gttgtcaccc tgtgagttaa gaggttgacc cgctctactg gacggccatg
     6961 acaacggtag tcactttctg ggctcactac cgttgtcacc ctgtgagtta agaggttgac
     7021 ccgctctact ggacggccat gacaacggta gtcactttct gggctcacta ccgttgtcgc
     7081 catatagaat gagaggagca tcagctcaac tgaacggcaa tgactacagt aatgagctgg
     7141 atgaagcata accattgaca tctggcccaa ccggaagccg tattcgccaa tccaatcgag
     7201 attcacaatg gccgtgactc gccgcattaa gttccggtgt cctcccaaag ctgaacgcac
     7261 actcggagga caatgacaat gccagtaagg tacacatcat taccattgtc actatagcta
     7321 ccagagaagc cgggatttga atgtcgatgg tagtgagtgg gtcaactcac tgccattgtc
     7381 accccgttac gatattttcc gttggatgta ctggccaatg aagtaaagcg tagaaaccgg
     7441 gtgttaacag gtgactcagg agaaagcgtt gaatttcgca atctcgatct acatagggtg
     7501 gtagagaaca atgacaacgg taatgacttt tgactcatta ccgttgacgt gaaagagcgg
     7561 gctgggacta acgaaaatcg aagaagtaat ttagcacccg gagcctaaac cgccgggtta
     7621 actttagctg gcgttgctgt tctttttctg gcttcgaaga ctacgaatat agcgcagttc
     7681 ttcagcggtt aaatcatgga cggtaagaac atcatcgccg tcaatcgggt cagctggtgg
     7741 gcataactca ccttctaaaa cagaagaccc tgagttctct tcttcatctt caaccacttc
     7801 taaaggaggc tgagtattgt tcaaattcag atcgggatcc cgcttatgaa tctggaagta
     7861 tacttttccg tctttcttaa tttcggtgta cttgaggtat cctatcgatg ccaactcttc
     7921 aagagccttt ctcacggtag cattctgact atttgctcgt gttttgaggt tcagacgatt
     7981 tttcagccga gtaagggata tcggtatagg atttggcggt aatgactcta tgaacgtata
     8041 gagagcctgg gcgctttcct tcttggccaa ctccttaatt gccttcagct gcatcaaaac
     8101 cttatgatcg tattgataga gctcgaaaat cttaggatca acctgaagga caacctgatc
     8161 tgattccggg tctaacaggg cagactgtac cagatgcgtt gtatagactt tggacgagtt
     8221 attactggtg aacgacaagg taacggatgc taacctgcgt aaggaggcgt ctatacgttt
     8281 tctggacgct gtagatgagc gataattttc aggcgtacat agcttgagga attcagtgaa
     8341 ttttaagctt atcgtgtcat tgttaatcgg atgattggca taggttcgaa tgatgcccat
     8401 ccacgtctta aaatctactg acatattaag acgctcaccg acaattgaaa tattggtgaa
     8461 cccctcgctc tccattaaag agagcgtctg taactctttc gttgcgtcaa agccaaccgt
     8521 tttatattcc cgattttgac gccccacact ctttggtgat gggacgaaca gcccgagccg
     8581 catcaaagca atagactgca ccgtacggtt gctgttagga cgcaaaatga tgtctttacc
     8641 tgattctttc tctctaacag agaaagccct ggtgaccagg tcctgctgct cagtatcttt
     8701 ctctttagtc ataataagtt atcactgaat gtgtaaacag attggtgaat agtaagcccc
     8761 agcgaactct atgtcactgg atggatcatt gtgatgatcc atatctgtgc ataagttcta
     8821 cctaaaaaaa cgactccact cattaaaact tggcaaaaac tctttgcata caatgacctg
     8881 caactcttat caacaacgga tcagatcaag ttaaaaagat ctaatgaaaa gtcactacta
     8941 ttgtcgctct gatcactact gatgtcaaaa aactcattac caacgttata ataatcacta
     9001 ctaatgtcaa aataatcact actgttgtca ctgatcacgt ctcaggcctt gtgccgtctg
     9061 gcctggaggc agcggggatc tcttttgatc tatcaggaac actaaggtac taattattgg
     9121 aactaccttg tggaaaatgg ggataaataa atgcttagtt cttatacagg tctcaacttg
     9181 cctccctgat aaaaactgtc gtcaaagcta aatcacagcc acaggcattt tcggtggata
     9241 aatttttgag aaatagaagc atacatgcag actcgtgtcg caagtaattg aaagtaatga
     9301 tttatttaaa taatgcgagt caccttacct cacagtggaa gtttaagtct aacctcacta
     9361 ccattgtcac ttaaaatcat taccaacgtc gaaatagtct ctaccattgt cttttaagtc
     9421 actcccgttg tcacttatgt cgctactgtt gtcagtaaca atgctccagc ccagtaacca
     9481 tgcggcctac agcgtaccgg gatcttcttt gtactctctg gcacctttgg gatcatttat
     9541 tggatctaca tgagggataa aatcttttga cgatttcgta gtgcagttat accagcgact
     9601 tagcaagaca aaagctgcaa tttagtgacg agttactagt ctgcccaaca agagcaatgc
     9661 agagaagata tgtggtgaaa tgaccatggt agtgagtcta atcactacca tggtccgcag
     9721 taagcgttac ttacgcaatg aacgtcggac tgatttgcaa caacatgggc tacggcgcat
     9781 cagaaggcgg ccgttgtgct tcagcaaaac agccctgctt tattttaatt cttcaatgtt
     9841 aatcatgaac tcagatctgc cagtaaggcc ggatacagca gggaaaatag gaaagctacg
     9901 gctgtccccg agccccaaat ccagttgaag ctctttgctt tcacctggca tcgcagaaac
     9961 gccatttaca gtcacgccga atgcagcgtc cttacggaca gtaagcactg cgtcctgaag
    10021 ccctgtagcg attgaaccat cctttttacg cagctggctg gcatacagaa cagccagagc
    10081 agtattgtca gatgttttga gagaagcatt gacagctaag gttttcagat tctctagcct
    10141 gacgagattg tttggaaggt agttaaattc gactgagctt tctttaacgt tgttcatctc
    10201 atctttggcc tgaactgtga gagtgtatgt ttcgccttca ttaagagacg gaaagatctt
    10261 gggataatta ggtgcataca ggttgtttcc cagattcacc caagacaatt ccaccgcatc
    10321 agaaacaggg ccaccgcgaa gagtcatacg gcttaaggat ggtttagtta gagcatcggt
    10381 gagctgtata cggacgttct ccagcccata tactgttacg tttttgctga tcggttttcc
    10441 ttcgtagcta atacttacag atggtgctgt attatcaacg acaactgttt gataagggag
    10501 gttaccggta ttgttgaatg tatcttgggc attaatcacc acgttataac tgccttctgg
    10561 tacctctttc atgttgaact catacgtgta gttcccagac gccatatttc tacctgtttg
    10621 tttgccggtt acgcttattt ctgcgttgtt acggttttta tctgacaacc aaactcgatt
    10681 tacataaaca tggtcgaaat atgaaccatc tccaggttga ttcacgtaaa cctggagaat
    10741 gttggtggtt tcattgtaat caaatccagt gacggaggga cccagtgtat gccaggctat
    10801 attctcccat ataggggcaa agaaggttgc ttcggtggtt gaccttactt catagccact
    10861 atgaagataa ccggtagtgc catttataat atcctggggc aaagagacgg tacaggttgt
    10921 tgagccagct ggaatactgc atgttgctcc gccagtgatt ttttgatcat atggcctggc
    10981 ctgtacattg aatcggatat tagtaagttt tataggcata tcagatgcct tgaacaggtt
    11041 ccagtttacg ctgttgaacc atgttccatc agccttttgt cgttctatgg gtgaacttcc
    11101 ccaagccgga ctctttacag aggcaggatc ccagttgagc cagctggcat actgaccaag
    11161 atctcctcca gaccattgat atgtattcac aggtctatag ttgtttccat ctaaggcacc
    11221 ctgtggcagc ttaacttcca cgtagacata ggtggcatct tcggaaagga cctgtactcc
    11281 gccatagtta ttggtaatgc tcatgccacc atttcgatat ggcttccagt tagttcttgg
    11341 gatacgtata accaacttgt aggggttttc cagcactgta aggcctcgtt taaacgggac
    11401 gtatcccgaa gaaatgccgg gtacaacact ggtgcttaca cgtgagtcat gaactgcaaa
    11461 tggcgtaaat tctcccattt gatcgtcata taaaaaccgc tggggaggaa tgttaagcgt
    11521 atttcctgcc aggtccgtaa cgatgaaatt gaaggtgaat tcttcatcaa gatcacttga
    11581 gggcatgcca gcctgggtgt tcatatcttt aatccacgga tagaacgcac gtttattggc
    11641 tgtgtcataa ctcaggttat tgtcggagac cacagaacca ttacttcgtt taatgacaag
    11701 acggatcttg tcaattccac tagcatcctc aataccgtct gcaaatatag aaaactggcc
    11761 actaccgcct cggcccaact cccaaagtgg cccagaaagt accatgtcgt aacctgcgtt
    11821 ttgactcgga taaatactct tatagcgtgg aggagttgta tcaacgatga aatgataagt
    11881 agatgtgctc actacagtct ggcgaatatc cagagtttca ttgactacag taaaggtccc
    11941 ttctccgagc gccggtagaa ccatatcctt tccatagtat tctgttccat cggcagcaac
    12001 gatacgatct gcaacgctcg ttttggtcga gacggaggaa tacataacct ttttatcgct
    12061 gtctcgtgtg accgtcactc gctcgtaccg atcaagaccc gatatcaagt tcagagtcaa
    12121 aacaccagcg ggattaagat atgtttgtgt tgcaggtttg atattccgaa cagccttatt
    12181 ggtatctgta aatgagtagc taaatacctg agcatgtgcc gaagatacag tagcaagggc
    12241 agttgcaacc atgcagccaa ttagagtttt acgcatatga tgctccggga ataggttata
    12301 gccttattct acggattcaa cctacaaggt ccggtcggat aaagtgataa tttgcctgtt
    12361 aaaagagaca tcaagtgaga aagcaaccaa cgtacagaat gcaaaatggc ggcagtgcat
    12421 ctggatgacg caaaagtaac tttttgtatc cacttttttg gattttagcc ttcaaaatga
    12481 gtattcaacc aatcataaaa gtaacttttg atagacgctg ctcctttatg tttgccaatt
    12541 gtgctgatat gtttgtaaat tcaaggaagc gagattttaa aagcatctgt tgagtcaaat
    12601 ttatgtaaaa taacagggaa accatcactt ctaggttgag gaaacccaat gttatacatg
    12661 ctcttatgct gttttctgat gctcaacagt acattcgtta tgttcagagc aatgtctgca
    12721 ataagtaaag gatccgctaa agaaaaccgg tctgaaatat cgctgatagt cctagcaaca
    12781 cttggaatcg catcaccatt cattgtcgca atgattacta ttaatgaaag tatgacatct
    12841 aaaacagtaa cagatttttc tttgggtgct cagtggtacg gaatggtttc agcggtagcg
    12901 ctaatggggt tatatgctcg acgcgtttgg aaagagaaga agtccttatt tactggcgct
    12961 ttcctggcct cttccttaat ggcctttatt tttactgaca gccttgtttt cgtatcccag
    13021 aaggacaccg gcgttctggc tactttcgta ctcgataaaa acgctggaga tattgattgt
    13081 agtcgcccgg ctatgattgt ccattacagt aaaggtgttc caacggactg gcgttgccca
    13141 accagtatca tgttgatggc gtattcttca tatccttttt taccctggcc tgagtatagc
    13201 catggtacca gccagtctct tacggttgtt attgatacat ttatggaaaa cgctgttaat
    13261 ctcagccaaa aataaaaaat aaacctctct agtgagaggt tttataaacc tgagccacca
    13321 atctaattaa ctctatattt ttgttttagc ttttttaaag atagacttac cgaataaaac
    13381 agcaattata gagcaaaggc ctggcgttag gacaaaagct aataatgcac tgccaaaact
    13441 tgtcatttca ggcaccagga accagctgat aggccacgtc agggctggta aatttagcgg
    13501 atcataaagc gcatgtagag cgcttaacac acagtaaagt gctaaaaagc aaactagata
    13561 aacaacgatc cacattttaa tttgctcagc aatttcaaat tttggactgc ccatgcttta
    13621 cctcagtggc tgaatattga tggttaatat ctttaatcac ccgtaagcac ttagtgcata
    13681 ccttagttcg atttgtctgc ttgtccagag tgtatttaac ttcaagttga cctccagcat
    13741 taagcaaagt gagcatctca tcttctaaca cgcttcggta aatggtcttg gccgagagtg
    13801 tgcccggtaa cgagtactca acataattgt ttgaaactct taccgattta gggattttac
    13861 cactgagctg ggtttcattc gttgaaggca ttacagttac tttaatcaca gttgccggtg
    13921 accagaaatc ctgataacgc actttatgaa ttttatcagc tctaaggcct aaccacacat
    13981 ttccggcgat aagaacaata agcaatccat aaactgatcg tcgaatgtta cgccttttaa
    14041 tcacaatgtc ccccatccct caaatatcat tctaaataac tgcaccagga tatcatgagc
    14101 gtaattaata gaattcttca taaccggggt tttcagttga gtttgcggac tctttacttt
    14161 gcggtttctc accctctagt tgagtatccc tgacaggctt ggatttaccc atgctcctct
    14221 gaaaggtatc cataacaaac tcaatatctt cttctggcag gctggtgggt tggttgctca
    14281 taggacttcc tcttgttgaa tacgttcggc gtacaggatg cgaaccgaaa cggtcgtgtt
    14341 ttcaaactcg ttctcaaaaa cgtccatcca ttccgtccgg aattcttccc ccaaaaggtt
    14401 atcgagcccc tgaagagata atggaagaac ggctgctaga gatcccccgg ctacgagatg
    14461 agatgcagct gcctggagat gcagcctggc ccggttatcg gcaaaaggtg ggttcatcac
    14521 gacataatca aacttcttct gttgattaga cttagaccat gtgaggaagt cagcttctgt
    14581 cgtatcgtat cccttagccc gggagataag gcaattcaaa gtatcaagct cgatgccagt
    14641 aacgatcaca ccggcaggga atgatttaag caaatccgca tggccaatat taggctccag
    14701 gagggtatcc ccttcccctg caccaagcag ggaagataca tattcgctga ttcggcaaga
    14761 tgaagggtaa aactgatgcg atacaatatc aggcatctcc cctatctggc cgatgtaacg
    14821 aataacctca cacggatcat aggaaaatgt cacatcgtat tttgttactg ttgcccctaa
    14881 gaaccgaagg gtgtcagcgg caacactcga agatttgcgt tcggcgagag accctaaaga
    14941 tgtccagcaa ctccatttat tgtccccgtc atttttaaac atcagttcag aaagctgcat
    15001 ccgggtatca aagtcgatgc actgcttaag tacagggaat gcctccaaag atttcttgct
    15061 atgagccata cggtcggcag gtaatgccag cggtacgata gctgataaaa tattgttaag
    15121 ccgctcggcg atatcagggt ggacgtcaat atgcattgat ccatttttgt aaaccctgaa
    15181 acgaatactg ttgccatcga tgcagatcca ttttctgaac ccctcatgtt cgaccaacgc
    15241 ctcaaccaat gatttggtgt tatggatcgc cttcacctgt tcgccacgga agaatgcgca
    15301 tataacgcgg agctcgctca gcggtgaaat gccagactct ttaaaatcca cccttaattt
    15361 ctgccagggg tatttagacg gctcgcaaac tccggtaggg atcatgcggg tggagaaccc
    15421 aaaagctttg tttgtcttgt gactgcggct cagagactgg aagacgtcgt aaaccctttc
    15481 tttaataaat tgattgcggt cattaagcaa agcaacgact gtgcccacta cagcgtcagc
    15541 tgtgaaatca gggatcacct gaggcggcat aatataacgg tcggccgtaa actgcttatc
    15601 ccattcgtta cgtttggcat ccgacatgat aggcaaaaca tcagtgagtg ccataacgcg
    15661 tttccagtat tctgatctca gagcgttttc tgcgaacaag gcatccgctt ctgagatgct
    15721 gtggttgtag cctgagagat aagaataaag tttctggtga ttgtgggttt tgaactggac
    15781 agccatgagt ttcaacaagc agagttcatc attataggac tcaacaagat ccgcgataat
    15841 gccgagctct ttgcgcatat ctggaagaaa tgtttcagga gagctaacca ccaggctatg
    15901 agaggttgtt aaagccagta cgctgtgacc tgcattatcg ggtttagctt cagaatcaag
    15961 agatgacatt ttaccggtcc tttagaaaaa acttttacca ggatagcaac gtcgctcagt
    16021 aacctaatag gttcagtgac acgaaaaagt tttttatgtc tctgaataaa acttaaagat
    16081 gtcaactcat agaaagaagc aaaaaaaaag ccccgcataa acggggcaag aacgatacat
    16141 ggaggtttaa aaaaggcggg caaaaagcct gaatccttaa tgactctgac aaaaccagca
    16201 ctttgccaaa agtaaattat tctgcgtccg gctgtgggtc agtatcctga tcggtctctt
    16261 cccctgcctt cttagcattt tttttacgtg ggtattttgg cttctcacca ttaagttttg
    16321 cctgacgatt ctccgcacgg cgaagctgct tgttctctga ttctgtgatc tcttttgaaa
    16381 gatttctcaa catctccaga gtactgcggt tcaacgtaat gttgatttct tcgtcagcac
    16441 ttgaatctgt cagctccggc aattcaatat cccccagttt ctgtaataag ctggtggtga
    16501 tgagtttgac gccttctacg gcttttgtgg caataaccgg cggaatccgt gctggcttga
    16561 agtctgaagg tctgactcgt acctgaccat gttgggtttt tttcaccgtg atgccattcg
    16621 catcggcctg ctcaagttct gcgatcatct tcttgatacg tgaaattgct tctgtttcgc
    16681 cgagctcatc gatcagtgaa agcacgttaa catacgaaat agcgtcagca tgaatccatt
    16741 tcttgataac aacagaacag gttgtcaggc ggataagaga actgatggtc gtcgggcttt
    16801 tattttcacg ttgagcaatt cgttcgagtg accagccagc aagagaatgt aggcgacgat
    16861 aggactcgcc aagcgcaaca ggagaaagcg caagaccacg gttaccatca atggtcgcga
    16921 gctcacagcc aatttcatct tctttaatct caacgcaaag gattgtgatg tcatgatctt
    16981 catcacatgc catcattgcg gcttttaaac ggcagtgccc ctggcgaaca aaaggtaccc
    17041 catcaatgac acgtactcgg atagggtcta catagtcacc tcgaatgtag gcattcttga
    17101 tgttgtagat gtgttcttca acctcaggga gcttatagta atcgtcaccc attcccattt
    17161 cgcgggagtt gaagcctggc gtgacctgaa ttactgaagg gtggatagcc catgctttct
    17221 ggtcacttac ttcagtacaa agttcagggt ttgcatcacg gaactgttta agcccagtga
    17281 cgtaacggcc tgtgccagta actactgaaa aggcaacatg ggccgtttta aagaccttgt
    17341 cattagcttg ctgtgtaggt tctgtcagta aaacactcat aggggctccg ttacattttt
    17401 ctgcttactt tctgcgtagc ggggatacta ccgaaaaaaa aattcataat ttttcgcaaa
    17461 aaaggtactt tttgagccta ttctgatttt ttacaaaaaa aggtggctcc ccattacttg
    17521 tgatggcata aaaatcgctt cataaacaaa tacataaatc caaaacataa agttcaggta
    17581 taagtgagga aaaatcttat aaatccgaaa tgctgaaagt caaaactgtg cgtcttaaac
    17641 aacttttttt tgctccctaa gttattagtt aaagcccgtg gttaggtacg ataccagcac
    17701 ctaaatttct ctctcaggta tagacacttt gactcgacat ctcagacaca tgccgataat
    17761 caaatgggct ggtggaaaaa cgaaactgat gcctttcatc agccatcact acccacacga
    17821 tcatagttgc cggtgggtag aaccctttat aggcggtggt gccgtttttc tgaacatgtt
    17881 tgcacaaaat gcattgcttg cagatagcaa tccagacttg atcaacctat acaggactat
    17941 tcaaagacag aaaactaact ttataaatca ggtccaaaac ttggcggata aaacctttgt
    18001 cgaaaaggac tactatgaga tgagggaccg tttcaacaaa acctgtattt ctggtcaatc
    18061 gcttcaaaga gcggctttgt tttactccct gaatcgcctg ggctataacg gtatgtgccg
    18121 ctataactca gaaagaatct attcagtgcc gtgggggaaa catacagaac tgaagcttga
    18181 cctcaataaa atagactacc tttcatttcg tctttcaggt attgaactga tcaccgctgg
    18241 ttttgaggag actctggccg caaccggtga aggagatcag atttactgtg atccgccata
    18301 tgacaaaaca agcaaaacta gttttgtcag ttacgatggt aaaccgttca gccaaagcga
    18361 ccacgtgctg ttagcaaata tgctcgttga cgctcacaga aaaggcgctg ctgttgcgat
    18421 atcaaatagc ctgacatcat tcactttggg cctctatgaa gagcgtggat tcgtcataca
    18481 cagactcagc gcttaccgat ccgtaggaag taagccgaat acacggaaaa cggaaacaga
    18541 aatactggcc gtgctgaaat agagtaaatt ctaatcaact aggctaatcc attcctcaaa
    18601 agttaatacc tcagggtaag ttcgttgaac ataaggtata aactgaagct cttctattct
    18661 ccggtcgccc ctctttcctt cgcaaataaa tgaaatgagt ccttgctgag aaaggccagt
    18721 aaggacgctc ataagtctta tatttagact tttaagctgt aagtcgttat taatgtcctc
    18781 cactttatga gagctctggc ctgaacgttt aagatactcg taaaccatga aattcgaatt
    18841 tcgtcgtttt aacaattttt cggttatgca ttcctgaatg aagtcgccaa gagcatctaa
    18901 aagcggactg agaactaccg ggaaagttaa tgtgtaactt tgagaagttt tagacaaagt
    18961 gaatgagttt agaaagctaa agtttagtct cttttttgaa tatggaacat aagcgacaag
    19021 agtagtcgac ataacagtaa gtaaagcttc aagatggttc actgttatta aagtggtttt
    19081 atctgtattt ctaagattat ggaaatcaat aaacgctact cttgaacggc taagccaatc
    19141 ttgtacttta atgtcgatag aaaattgcga gatatgattt gaacattcat tttgggtgat
    19201 agggcatgca gacgaaagat ggttatggat tatcatgcat agcacaaaaa aaggaaaacg
    19261 gcaggctgtg gggcattgaa aaccggttaa agaaagctta gacgtttcat atgcgcttac
    19321 cggaaaagta acttttctta acgttggctt tgacgatgtt cttgcatttt tacaaatgaa
    19381 accgagaccc agatcattgt gcattttaaa gtccatctcc atcatcacct t
//
LOCUS       DAPGOF010000090        18849 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.090, whole genome shotgun sequence.
ACCESSION   DAPGOF010000090 DAPGOF010000000
VERSION     DAPGOF010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.090"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(44..1045)
                     /locus_tag="RHV13_002769"
     CDS             complement(44..1045)
                     /locus_tag="RHV13_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDV1242290.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWVTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSTSCCTPAK
                     TSPGTTYTPIKVSCCNPVAKAIEKPVVNCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSAWIAAHAGTDNPFAIPLSAVIGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGASLTEVILLKSMFRIPMIVAFLTVILGMAILMGYLTQMLF"
     gene            1152..1472
                     /locus_tag="RHV13_002770"
     CDS             1152..1472
                     /locus_tag="RHV13_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001614035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV1242291.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKTLGEGA
                     TKNK"
     gene            complement(1493..1918)
                     /gene="arsC"
                     /locus_tag="RHV13_002771"
     CDS             complement(1493..1918)
                     /gene="arsC"
                     /locus_tag="RHV13_002771"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HDV1242292.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTTLG
                     TRLCRPSEVVLEILPDAQKGAFAKEDGEKVVDEAGKRLK"
     gene            complement(1931..3220)
                     /gene="arsB"
                     /locus_tag="RHV13_002772"
     CDS             complement(1931..3220)
                     /gene="arsB"
                     /locus_tag="RHV13_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HDV1242293.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSTDFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMIYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(3275..3628)
                     /gene="arsR"
                     /locus_tag="RHV13_002773"
     CDS             complement(3275..3628)
                     /gene="arsR"
                     /locus_tag="RHV13_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HDV1242294.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVHN
                     LARQNCSADSKNICS"
     gene            complement(4253..5461)
                     /locus_tag="RHV13_002774"
     CDS             complement(4253..5461)
                     /locus_tag="RHV13_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV1242295.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTAFEPVNSFQKNQKKLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SASGTVSLPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSQFRCPVCGYTANADVNGARNILAAGHAVLACGGMVQSGRPLKQ
                     EPTEMIQATA"
     gene            5484..5899
                     /gene="tnpA"
                     /locus_tag="RHV13_002775"
                     /pseudo
     CDS             5484..5899
                     /gene="tnpA"
                     /locus_tag="RHV13_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016192838.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            5966..6034
                     /locus_tag="RHV13_002776"
     CDS             5966..6034
                     /locus_tag="RHV13_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HDV1242296.1"
                     /translation="MIVLGALIALLELIRFLLQLLN"
     gene            complement(6088..7440)
                     /gene="gorA"
                     /locus_tag="RHV13_002777"
     CDS             complement(6088..7440)
                     /gene="gorA"
                     /locus_tag="RHV13_002777"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HDV1242297.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFIDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(7512..8354)
                     /gene="rlmJ"
                     /locus_tag="RHV13_002778"
     CDS             complement(7512..8354)
                     /gene="rlmJ"
                     /locus_tag="RHV13_002778"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HDV1242298.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            8557..10599
                     /gene="prlC"
                     /locus_tag="RHV13_002779"
     CDS             8557..10599
                     /gene="prlC"
                     /locus_tag="RHV13_002779"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HDV1242299.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTQALNDCRENVERVVAQG
                     APYTWENLCQPLAEVNDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYNTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYALKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            10607..11359
                     /gene="rsmJ"
                     /locus_tag="RHV13_002780"
     CDS             10607..11359
                     /gene="rsmJ"
                     /locus_tag="RHV13_002780"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HDV1242300.1"
                     /translation="MKICLIDETGTGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(11408..12877)
                     /gene="dtpB"
                     /locus_tag="RHV13_002781"
     CDS             complement(11408..12877)
                     /gene="dtpB"
                     /locus_tag="RHV13_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HDV1242301.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMVAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(13195..13629)
                     /gene="uspA"
                     /locus_tag="RHV13_002782"
     CDS             complement(13195..13629)
                     /gene="uspA"
                     /locus_tag="RHV13_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HDV1242302.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            14020..14355
                     /gene="uspB"
                     /locus_tag="RHV13_002783"
     CDS             14020..14355
                     /gene="uspB"
                     /locus_tag="RHV13_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HDV1242303.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(14426..15925)
                     /gene="pitA"
                     /locus_tag="RHV13_002784"
     CDS             complement(14426..15925)
                     /gene="pitA"
                     /locus_tag="RHV13_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HDV1242304.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            16157..17359
                     /locus_tag="RHV13_002785"
     CDS             16157..17359
                     /locus_tag="RHV13_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDV1242305.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVEKDDTGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLSTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIVEKSS"
     gene            complement(17674..18726)
                     /locus_tag="RHV13_002786"
     CDS             complement(17674..18726)
                     /locus_tag="RHV13_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HDV1242306.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWTLLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT         4846 a         4789 c         4832 g         4382 t
ORIGIN      
        1 aagacactca caatgctcct tatagtttac tgctggcagc tatttaaaat agcatttgag
       61 tcaaatagcc cattaagata gccataccta agataaccgt caggaatgca actatcatcg
      121 gtattctgaa cattgattta agtaatatca cttccgtcag gcttgcgccc gcactaccga
      181 tgattaatgc cattaatgct cccagaccca ttccttttgt catcaaaaca gatgccagag
      241 ggataactgc ctctgcccgg atatacagcg gaataccgat aacggcgctc aatgggatgg
      301 cgaagggatt atctgtccct gcatgagcgg caatccatgc agagggaata aagccataaa
      361 taaaagagcc tattaatacc cctaacaaaa gatatggcag tacatcttta aactgttgca
      421 acgcatcttt ggtggctagt tttatgggat taatacttac cacagctttg gtactgcaac
      481 agttaacgac gggtttctca atggctttag ccactggatt acagcaactc acttttattg
      541 gcgtatatgt cgtccccggc gaagttttgg ctggagtaca acaacttgtt gatgagcttt
      601 tactggcaat gatatgacgt tcaaacccca gagaatccag gataatactg gcaagtacgg
      661 agacgccggc ggcgataatc gcgtacaaca aggtaacttt ccagccaaag gtcacccaca
      721 ttagtccgac gataatggga ttaagtaacg gagaaacaaa taaaaaagtg agagtcggac
      781 cgaacccagc tttcgctgac aacaatccac gtaacatggg tattgtcgag caactacaga
      841 acggggtaac ggctcccaac agagcagcca ggagatagcc tctccctttt ctggccccca
      901 tcatctgctg gattttatgg tctggcacct tttgtcttat caggctgaca ccggcactaa
      961 tcactataaa cagcaaagaa agctcgacgg cgagaaacac aaacatctct gcggcatctt
     1021 gcagcatcgc aagccaacta ctcatacata acctcattga ataaatctat atttccagaa
     1081 taatcgaaat gaaattcaga tcaagatatt tctggtatta tcgaaatatc aaccagacag
     1141 aggggtgtca tatgcagtta gaagaagtcg ctaaagcgct gaaagagcta ggtcacccga
     1201 cccgtttgtt catatttaaa catcttgtca aagcagggga acaagggttg ccggtcggtg
     1261 agttgcaaaa gcagcttggg atacctggct ccacactcag ccaccatata tcagcactgg
     1321 tatcggtagg actggtcaaa caaaaccgtg agagtcggac gctgatgtgt gtttcgcaat
     1381 acgcaatgct ggaagcgata attgaattct tacgcgagga gtgctgcgta aatagcaaaa
     1441 cacttggcga aggggccacg aagaacaaat aagggggcat atccccctta agctatttca
     1501 ggcgcttacc cgcctcatca accactttct cgccatcttc cttggcgaac gcgccttttt
     1561 gcgcatctgg cagaatttcc agcaccactt ctgaagggcg gcacaggcga gttcccagcg
     1621 tcgtcaccac aatcgggcga ttaatcagaa tcgggtgctg aagcataaag tcgattaacc
     1681 gatcgtcagt aaatttatct tccgcaaggc ccagctcctc atacggttcg acgtttttac
     1741 gcagcagcgc gcgtacggaa atccccatat cggcaataag tttgaccagt tcatcgcgcg
     1801 ttggcggagt ttccagataa tggataatgg tcggttctgt gccgctgttg cggatcatct
     1861 ccagcgtatt acgcgacgtg ccgcaggccg ggttgtgata aatggtaatg ttgctcatat
     1921 cagtatctca ttacaaagtg aaagagagac gtagcgccag cgcagccagc gtcacaaaca
     1981 gcacaggcag ggtcatgata atccctgtac ggaagtaata tccccagctg atagtcatat
     2041 tcttctgcga aagtacgtgc agccagagca gtgtcgccag gctaccaatc ggggtgattt
     2101 tcggccccaa atcgcagcca atcacgttgg cataaatcat cgcttctttg ataacgccag
     2161 atgccgtgct gccatcaatg gacaacgcgc caaccagtac cgtcggcata ttgttcataa
     2221 tagaagagag gaaggcggtg aggaatccgg tgccgagcgt cgcggcccac aggccgttat
     2281 ccgccagcac gttgagtacg ccagaaagat attccgttaa tccggcattg cgcaggccat
     2341 aaaccaccag atacatgccg agcgagaaga tgacaatctg ccagggtgca ccgcgcagga
     2401 ctttacccgt attaatcgca tgaccgcgtt tagcgacgac aaacaaaatc agcgcgccca
     2461 cagctgcaat ggcgctcacc ggaatgccga gcggttccag gacgaaaaat cccaccaaca
     2521 gaagcagtaa aacaacccag cctgttttga acgtagcagg atctttaatc gcttctgcgg
     2581 gagatttcag cagcgccatg tcgtagttct gcggaatatc tttgcggaaa tagagatgca
     2641 gcatcaccag cgtggcaaca atcgcggcga tatccaccgg caccatcacc gaggcatatt
     2701 cgcgaaagcc gaggccaaag aaatcagtgg aaacgatatt caccaggttg gagacaataa
     2761 gcggcaggct ggcggtgtcg gcaatgaatc cggccgccat cacgaacgca agcgtcgtgc
     2821 ctttactgaa tcctaaggcc agcagcatgg cgatgacaat cggcgtcaaa ataagcgccg
     2881 cgccatcatt ggcaaacagg gcggcaacgg cggcaccgag caggacaatc caggtaaaca
     2941 gcaagcgacc gcgtccattg ccccagcgtg aaacgtgcag cgccgcccat tcaaaaaagc
     3001 cggactcatc cagcagcagg ctgatgataa tgacggcaat aaacgcagcc gtcgcgttcc
     3061 agacgatatt ccacaccacc ggaatatcgc ccggatgtac tacgcccgta actaacgcca
     3121 gtaccgcgcc gagcgttgca ctccagccga tgcctaaacc tttcggctgc cagataacca
     3181 atacaatggt caggacaaag atagcgcctg ccagtaacat aatgcctccc ggataaaaca
     3241 catgtgaaaa tccatatgtg tttagctaaa ttttttaact gcaaatgttc ttactgtctg
     3301 cggaacagtt ttgtcgggcc aggttgtgga caatcgcctg aaccttttcc tgttcacatc
     3361 gccaggcttc atcaataatt ttcgccgccc atgctggaat atgcggcgat aagcggtaat
     3421 gaacccactt accttgcttg cggtccagca atagcccgct ttcccgaagt aacgccaggt
     3481 ggcgggatat cttgggctgc gactgatcga gggcagtgca gagatcgcag acgcataact
     3541 ctcccagttc gctgagcagt aaaacgatgc ccagacgggt ttcatcagca agaattttga
     3601 acaattggat gggtaacaga aatgacatat tgcgctcctg attgttgcag gtagtgtctc
     3661 tcttcgaagc ggataagtca aaaacatata tgacttaacg aatatgtaag tgtagagaaa
     3721 ggtataggtg tgaattttga gttgggtatt catttgaaag gaggtctgga tcaggacgta
     3781 aaaaaaccga ctttgcgtcg gtttttactc ttcagcctga gttggtggct ctggccggag
     3841 tgagaatgcc cacatctaac ggttcaatac tattagccag tggctaacat tgcaagcgtt
     3901 agcgatttct tcagctggag aagggacagt ttgctataca tgttggtgaa agaggattta
     3961 tatacgttca tcgggtcgct gccgtaccaa gccttgtccg aatccggacg taaaaaaacc
     4021 gacttcgcgt cggttttttt actcttcagc ccagagttgg tggctctggc tggagtgaga
     4081 tagccatcat ctaacgcatc aatgctaata ccatactgaa actattgcaa ggacgtgctg
     4141 gttctataac ctgcatgtac tgtatgatta tccagttagc tctgaggcat tttcactctg
     4201 gcaatgcgct tgacatcctc cacgccctaa aggacgtgga ttcctgctac gttcaggctg
     4261 tcgcctgaat catttcggtg ggttcctgct tcaacgggcg gcctgactgc accatccctc
     4321 cacaggcaag aacggcgtgc cccgccgcta aaatgttacg agcgccgttt acatcggcgt
     4381 tcgctgtata tccacatacc gggcatctga attgactttg tgacaggcga ttttctttcg
     4441 ctgtatgacc acagcacgcg caacgctggc ttgtgtacgc tggcggaaca gcaagcacct
     4501 gaccgccacg ccagagctgc ttgtactcaa gctggcggcg catttcatac cagccctgat
     4561 ccagtatcga acggtttaaa cctgattttg cccggacatt gcgccccggc aggctgaccg
     4621 tacccgatgc tgactttgac atgttgctga ccttcaaatc ctcaatgact atcattgcgt
     4681 ggtttttgct gacggtcgtt gtgactttgt gaaggtagtc cctgcggata tttgcgatac
     4741 aggaatgcag tcgctgtatt ttgcgtttct gcttctgcca gttgttgctg aatttgacct
     4801 tgcggcttaa ctgtcgctga agtctcgcca gcttcttctg gtttttctga aaactgttta
     4861 caggctcaaa ggctgtgcca tctgacagcg tggcgagttt agccacgcca gcatccagcc
     4921 cgaccattga tgctgaaggg tgaaccggag ttgatacttc actttctgtc tgaatactga
     4981 tgtaccactt accgcaggac tggctgaccg tgacattttt cacaacaccc gtgacctgac
     5041 ggctattccg gtagcgcatc cagcccagtt tcggcagaaa aatacggctg ttttcctgat
     5101 cgagcttaac gccctgcggg tagcggaatg catcattctg tccccgcttt ttgaatcggg
     5161 gaaaagcagc ccgcttctgg aagaagtttt tgtaagcccg ctcaaggtct ttcagtgact
     5221 gttgcaatgg ctgtgagggc gaatctttaa gccattgcgt ttcagtggcg tttttccact
     5281 caaccagcca ggaagccatt ttcccgtaag ggatgtattt attaccggcc tcatgattct
     5341 cattctgacg tgccagagca cgattgaaaa cgaaacgaca tgcgcctgcg aagcgcctca
     5401 tttcacgctc ctgttgacca ccgggtctta actggaattt aaatgcctgt agtcgtttca
     5461 tgggccaaat catactttgg cctatgaaaa aagaaaccga tatccgacgc ggaaggcatt
     5521 gtgttttcct gatgcatgtt cacctggtct tcgttactaa ataccgacgt cagatttttg
     5581 actatgacgc gacagaaaaa ctacgcactt acttttcaaa tgtatgtgct gattttgaag
     5641 ctgaactggt tgaaatggat ggcgaaccag atcacgtcca tttgttaatt aactatcctc
     5701 ccaaactggc gatatccagt ctggtaaaca gcctcaaagg cgtatcgggt aggttactgc
     5761 gacgagatcg accagatatt gcagccaggt attactacaa agcgttttgt ggagtcctgg
     5821 ctattttgcc agtagctgcg gtggtgcacc aatatccatc atccgccaat acattgaaca
     5881 acagcaaaca cctggttagg tggaaaaccg caccttatat ccccgccctg aatgaacggg
     5941 gttttacggc gcaccggata aagcaatcat cgttcttggg gcgttaattg cgctgctgga
     6001 actgatccgc tttctgcttc agcttctgaa ctgatagcgg aaacgtaatt aagggctaag
     6061 agcacactac tcttagccct ttaacattta acgcattgtc acaaactctt ctgccgccgt
     6121 tgggtgaatg gcgacggtat tgtcgaagtc ttttttggtt gcgcccatct tcagcgccac
     6181 cgcgaagccc tgcaacattt cgtccatacc aaagccaatg ccgtgaatac cgacaatctt
     6241 ctcttccggt ccaacgcaca ccagcttcat gcggcacggc tggcggtgag tggtgacggc
     6301 ggtatacatc gcggtgaaag aggatttata cactttcacc tgatcgtcgc cgtactgctc
     6361 gcgcgcctgc ggttccgtta aaccaacagt accaatcggc ggatggctga agaccacggt
     6421 cggaatgttg ctgtaatcca gatgctcatc cggcttgtta ttaaataggc gttcagagag
     6481 gcggcgaccc gctgcaactg ccaccggtgt cagttctact gcacccgtgt tatcgcccac
     6541 cgcgtaaata ccttcaatat tggtgttttg atatttatcg acgacgatat agcctttttc
     6601 gttagtttta acgccagcgg cttccaggtt gatgttgtca ttggcaggct cgcgaccaat
     6661 cgcccaaatc aggcaatcca ccgtttcact gcgaccatct tccagctcca gcgtcaggct
     6721 gccatcggca tttttcacta ccgctttcgg gatggcgttg gtgtgcaact gcggaccttc
     6781 ggcgttcatc acttcgacca gcgtttcaga gatcatcggg tcgaagctgc gcagcggcgc
     6841 atgtttacgc acaaacagat gcgttttcgc gccgaggccg ttaatcacgc cagccagctc
     6901 aacggcgatg taacccgcgc caacaaccgc cacgcgctct ggcaaagcag gaagggcgaa
     6961 gaagccatca gaatcaatac cgtattccac gcccggaata tccgggtggc tcggacgacc
     7021 gccggtggcg atcagaatat gatcggccgt gatggtttca ccgttaactt ccagcgtttt
     7081 ggcatcaatg aagcgggcaa aacctttgat tacatcaacg ttatttttac cgagcacgtt
     7141 ttcataggaa gtatgaatac ggtcgatata ggcggtacgg ctggcgatca acgtttccca
     7201 gttgaattta ttgatagtgg tatcgaaacc ataatccgga ccgtacatgt ggatcgcttc
     7261 acggatttgc gccgcgtgcc acatcacttt cttcggtacg cagccgacgt tcacgcaggt
     7321 gccgcccagc tctttggctt caatcagtgc gcatttctgg ccgtacatag ccgcgcggtt
     7381 gatggaggcg ataccgccgc tgccgccgcc gatggcgatg taatcatagt gtttagtcat
     7441 gacagagtgt ccttatcgtt gattaccgcg attgtagcgc gagacgtaat aggtgccagc
     7501 aatggctgca attactccgg cacgatccag cttacggtgg cgtgcccggt gcctgccgga
     7561 accagtttgc tgtgcagcca cggcagcacg ttattcatct gttgttccag cttccacggc
     7621 gggttaatca caatcatgcc ggaagcggtc atgccacggc gatcgctgtc tggcaatacc
     7681 gccagttcaa tttgcagaat tttgcgaatg ccggtcgctt ccaggtcgtg gatcatgcgc
     7741 ttgatttgct gacgcagcac caccggatac cacaatgcgt aagtgcccgt ggcgaaacgt
     7801 ttgtaacctt ctgctatccc actgactacc gcctgatagt cggttttcat ttcatacggc
     7861 ggatcgataa ggattaaacc acggcgagaa accggcggca gtttggcctt aagctgctgg
     7921 aaaccgtcgg ctttttccac gcgcgcacgg ctgtctttct gaaattcaga acgcagcaac
     7981 gggtaatcgc tcgggtgcag ttcggtcagt tgcagactgt cctgctcgcg cagtagctgg
     8041 cgagcaatca acggcgaacc ggggtagtaa cgcaactgac cgctacggtt gaagtgtttt
     8101 accacattga tgtacgcctc cagctccgca ggcaaatcgt cctgctgcca gatacgggcg
     8161 ataccttcga gatattcgcc ggtacgctcg gcatgttcgc tacctaactg ataacgcccg
     8221 gcccctgcgt gggtgtcgag atagagaaac ggtttatctt tctctttcag cgactcgatg
     8281 atcaggctct gaacggtatg tttaaggacg tcggcgtggt tgccagcgtg aaagctgtgg
     8341 cgataactga gcatgggtaa aggtgttccg gtaagtaaaa atcggcccgt attcggggcg
     8401 cacaaaagcc tatcaggaca gtataccgaa aagaggccgc cgccgcgaaa gcgtaacgtt
     8461 tctcattgaa attcactaca cttaacccca tgctacacac attatgtaaa gcgcctgttg
     8521 agcgcttcct taacctcttt aaccaggact gcgctaatga cgaatccgtt actgactccc
     8581 tttgaattgc ctccgttttc taaaattctc ccggaacatg tcgttccagc cgtgactcag
     8641 gcgctgaacg actgccggga aaatgtggag cgcgtagtag cgcaaggggc accgtacacc
     8701 tgggaaaatc tctgccagcc gctggcggaa gtgaacgatg tgctggggcg tatcttctcc
     8761 ccggtcagcc acctgaactc ggtgaaaaat agcccggaac tgcgtgaagc gtacgaacag
     8821 accttgccgc tgctgtcgga atacaacacc tgggtagggc aacatgaagg gctgtataaa
     8881 gcgtatcgcg acctgcgcga tggcgatcat tacgccacgc tgaacacagc tcagaaaaaa
     8941 gcggttgata acgccctgcg tgattttgaa ctctctggca taggtctgcc gaaagagaaa
     9001 caacagcgct acggtgagat tgcgacccgt ctttccgagt tgggcaacca gtacagcaac
     9061 aacgtcctcg atgcgacgat gggctggacc aaactcgtca ccgacgaagc ggaactggcg
     9121 gggatgccag aaagcgcgct ggctgcggca aaagcccagg ccgaagcgaa agagctggaa
     9181 ggttatttgc tgacgctgga tattccaagc tacctgccgg taatgaccta ctgcgacaac
     9241 caggctctgc gtgaagagat gtatcgcgct tacagcaccc gcgcctccga tcaaggcccg
     9301 aacgccggta aatgggacaa cagcaaggtg atggaagaga tcctcgctct gcgtcacgaa
     9361 ctggcgcaac tgctgggttt tgaaaactac gccctcaaat cgctcgccac taaaatggca
     9421 gaaaacccgc agcaggtgct tgatttctta accgatctgg caaaacgcgc gcgtccgcaa
     9481 ggtgaaaaag agctggcgca actgcgtgcc ttcgctaaag ccgaatttgg cgtcgatgag
     9541 ttgcagccgt gggatatcgc gtactacagc gaaaaacaaa aacagcacct ctacagcatc
     9601 agtgacgaac agctgcgtcc gtacttcccg gaaaacaaag cggttaacgg cctgtttgaa
     9661 gtggtgaaac gtatttacgg catcaccgct aaagagcgta aagatgttga tgtctggcac
     9721 ccggatgtac gtttcttcga actgtatgac gagaacaacg aactgcgcgg tagcttctac
     9781 ctcgacctgt atgcccgtga aaacaagcgc ggcggggcgt ggatggatga ctgcgtaggc
     9841 cagatgcgca aagctgacgg ttctctgcaa aaaccggtcg cgtatctgac ctgtaacttt
     9901 aaccgcccgg taaatggtaa accggcgctg tttacccatg acgaagtgat caccctgttc
     9961 cacgagttcg gtcatggtct gcatcatatg ctgacccgca tcgaaaccgc cggtgtatcc
    10021 gggatcagtg gggtgccgtg ggatgcggtc gaactgccga gtcagtttat ggaaaactgg
    10081 tgctgggagc ctgaggcgct ggcgtttatc tctggtcact acgaaaccgg cgaaccgctg
    10141 ccgaaagagt tgctggataa aatgctggcg gcgaagaact accaggcggc gctgtttatt
    10201 ctgcgccagc tggagttcgg cctgttcgat ttccgcctcc atgccgagtt ccgcccggat
    10261 cagggagcga aaatcctcga aactctggca gaaatcaaga aactggttgc cgtagtaccg
    10321 tctccatcct ggggccgttt cccgcacgct ttcagccata ttttcgccgg tggttatgcg
    10381 gcaggttact acagctacct gtgggctgac gtactggcgg cagatgcttt ctcgcgcttt
    10441 gaggaagagg gcattttcaa ccgtgaaacc gggcagtcgt tcctcgacaa cattctgagc
    10501 cgtggcggtt cagaagagcc gatggatctg ttcaaacgct tccgtggtcg tgaaccgcaa
    10561 ctggatgcga tgctggagca ttacggtatt aagggctgat cattcagtga aaatctgctt
    10621 aattgatgaa acaggcaccg gagacggtgc cttatctgtt ctggcggctc gctgggggct
    10681 ggaacatgat gaagacaacc tgatggcgct ggtattaacg ccggaacatc tggaattgcg
    10741 caagcgtgat gagccaaaac ttggcggcat ctttgttgat tttgttggcg gagcgatggc
    10801 gcatcgtcgc aaattcggcg gtggtcgtgg tgaggcggtg gcgaaagcgg tgggcattaa
    10861 aggcgattat ttgccggatg tggtggacgc taccgcaggg ctggggcgcg atgcctttgt
    10921 gctggcttcg gtcggctgcc gcgtgcggat gctggagcgt aatccagtcg ttgccgcgct
    10981 actcgacgac ggcctggcgc gtggatatgc ggatgcggaa atcggcggct ggttgcagga
    11041 gcgtttgcag ttaattcacg cctccagcct gacggcgctg accgatatta ctccgcgccc
    11101 gcaggtggtc tatctcgacc cgatgttccc gcataagcag aaaagcgcgc tggtgaaaaa
    11161 agagatgcgc gtgtttcagt cgctggtggg accggatctt gatgccgatg gattactgga
    11221 gcctgcacgc ttgctggcga ccaaacgcgt ggtggtaaag cgcccggact acgcgccacc
    11281 gctggcgaat gtcgccacgc caaacgcggt agtcactaaa gggcataggt ttgatattta
    11341 tgccggaacg ccagtgtaaa agtaaagcag ggtagcgtta tcgctaccct gctttctgca
    11401 agaataatta atggctttcc ggcgtcgcaa tcatgcgttt cagccacggc accatcaaca
    11461 gcatcactac tgcaacgccc agtgtgacca gaccaatctt accaaacacg ttggtataga
    11521 caggcaacgt ctcaagcgga tcggtaatgt tgtctggtac tgcggtaaat gttgccacat
    11581 agccgcccag caagaacgcg gcagcctgcg tcaggaacca catcccgaga ataaagccca
    11641 tcaaatgttg cggcaccaga gcggcaacca tcgccagacc cagggcgcta ataaacagtt
    11701 cacccaggct ctggaacaag taaaccagca cgataaacca cggcgatgtc agtccttgtg
    11761 catcagcaaa ccacattccc gcagctgccg ccgtcaaaaa gcccagtgag cacataaaca
    11821 tgccgagagt aaatttcatc ggcatcgaga ggtctttgcc tttgttaccc agatgcgtgt
    11881 aaatgcctgc cagtattggg ctggcgagta ccacccagaa cgggttaagc gcctggaagc
    11941 tgaccgggtt gatggaaaaa ccgagaattt catgatgcac gttgttgatg gcaaagaagt
    12001 tcagcgatgt tggcatctgg gcgtagagaa tgtaaaacac caccgcttcg agcatcagga
    12061 caaaggcgac aaacatttta ttgcgcccgg ttttatccag cttgaatgcc tgacgaaaga
    12121 agatgatggt gacgacgatg gagagaacaa tcagcaccag attggcgact tctacgttgt
    12181 gcatcagcca ggcgcagacg aagatcatca ctacgctgcc aagtaatacg tacaacagtt
    12241 tgctgaagct cattggccgg aagtcgggct cagaaccaat gtctttcacc attccacgac
    12301 aggcgatgta aaccagtaat gcgataatta accccgcccc gcacaggttg taggtgacag
    12361 aataaccgaa tctatcagcg atcacagggg ccagcgataa cgctatcaac gagccgatgt
    12421 tgatcgacat atagaacagg gtgaatgcgc catcaaggcg cggatctttc ggcggatagc
    12481 acttcgaaag caagctggct gggttagctt taaacaggcc gttaccgaca gcgatagtcc
    12541 ccagggcaat gaaaatcagg tcaggcttaa gtagcgacat gccagtcatg aagtagccaa
    12601 tcgccagcac aagtgctccg aggacaatgg tgcgtttggt ccccagcagg tggtcgccga
    12661 catagccgcc aatggaaatg aggccataga ccagcgcagc aaaagcacca aaagtgacaa
    12721 aagcctgttc ttgcgagaat ccaagctgct taacgaagaa aaccgccagt acgccctgca
    12781 cgccgtagta gccgaatcgc tcccataact cgacaaaaaa gatcatgaaa aatgggcgag
    12841 gttgctgcag catccccatg ggtgttgttg tattcatatt tattaacctt ccaataccat
    12901 cctgaaaagt atgaacgcgt aagggataac gcgcgaaaaa gtttacatct gtcagagatt
    12961 cttaatattg atgcggtgaa tagattaata cactattaca acagaaaata accagctgat
    13021 cacactaatg atggaaaata tgatggtgtt aaatattgta ttggctgggg attttgattg
    13081 cgtgcgcaga tcgtaggcct gataaggtgt ttacgctgca tcaggtaaca aggcgcgcca
    13141 aaaagcaaaa cgcccgcata tgcgggcgtt caggaacacg tcgtggaggg aagattattc
    13201 ttcttcgtcg cgcagcggaa caatcagcat atcaacgtga acggtgttga tcagctgacg
    13261 tgcggaagac atcagtttgc tccagaagtc ctggtggtga ccacaaacca ccagatccat
    13321 atcgtatttc ttgattgcat cgaccagaac ctggcccagg tcgccgctgc cgctcagggt
    13381 ttcagtgatt gggtagcctg cattagtgga aagctcggtc agcgcatgat gtgtctcttc
    13441 agagatgcgt ttctgcatat caccaagatt cacatcaata agcccggtat ataggtcaga
    13501 gtagtttaca tctacgtgga tcagagaaac tttcgcattg tatgggcgag ccatagagac
    13561 tgctttctct accagaactt tgctttccgg ggagagatcg accgcgatga gaatgtgttt
    13621 ataagccata gtgttactcc ttccataaag ttgtcgatga ctggccagct agcgtttctt
    13681 gtgccgttac gtcacccgcg tcctgcgaac tacgtgcgcg gggctaaccc agccgtaaag
    13741 attcagtgtt aagaccatcc ttaccttata gcgacccgga tgatccgtca atccgccttg
    13801 cttacccgtt agataaacaa attttcgaca tgaatatatt gatagtggtt aaccttctgg
    13861 aaaaaaaaca acctgatctc ctacactatc tatagagccg ctcggatgtt ggtcaccaca
    13921 aaagcggttt ctccaggacc tggtgttaac tgattggcag cgtatcggga gcggtagccc
    13981 cggggtggaa ctccgcgggc aggtcgccgg ggaggaggta tgataagcac cgtcgcatta
    14041 ttttgggctt tatgtgtcgt ttgcattgtt aacatggcgc gctatttctc atcactacgc
    14101 gcgttgttag tggtactgcg taactgcgat ccattgctct atcaatatgt tgatggaggg
    14161 ggctttttta cctcacatgg ccaacccaac aaacaggtgc gtctcgtttg gtatatctat
    14221 gcccaacgtt atcgcgatca tcacgatgat gagtttattc gccgctgtga gcgggtgcgt
    14281 cggcagttta ttctgaccag cgcattgtgt ggtctggtgg tggtcagcct gattgcattg
    14341 atgatttggc attaagcact tcagcaataa taacgcataa aaaaagcggg ccagtaaact
    14401 gacccgctcg ttttggtgcg tacgattaca ggaactgcaa ggagagccag taaagcccgc
    14461 cggaaagcag taccgcagcc ggaagggtaa acacccaggc catcagaata ctggttacgg
    14521 ttttacgctg taagccgccg ccatctacca ccatcgtccc cgcgacagaa gaggagagta
    14581 cgtgagtagt ggaaaccggc atcccggtat aactcgccag gccgatagac actgccgcag
    14641 tcatctgggc agacatcccc tgagcgtagg tcatgccttt cttaccgatt ttctcaccga
    14701 tagttgttgc cacacgacgc cagccaatca tcgtaccgat acctaacgcc agcgcgaccg
    14761 ccatgatgat ccacaccggt gcatactcga tggtgctaag catgtcggac ttcagtttct
    14821 tcaacaggcg ctgatcgtca gcactcacgc caggcatctt caccactttg tcgatagtgt
    14881 cagaaacgca cagcataatg cggcgcatct ggctacgttg atcaagcgac agcttgtcgt
    14941 agctttccac atcggtagtc aacatgcctt tcaggcggtt gagcgcgtta atggtattcg
    15001 acggatgaca atggaactcc gcaggttgcg ttgcgccagc ttccggagcc ggtactaact
    15061 gatcagcacc ggtagcttgt ttcagcagcg caggatgctg ctcaaagtaa gcttcgacgt
    15121 tgttgatggc atcacgggta cgggtgattt cgtagccagt ggcattcatg ttcaccacga
    15181 agcctgctgg cgcgacgcca atcaatacca acataaccag accaatgcct ttctgaccat
    15241 cgttcgcgcc gtgcgaaaac gccacgccga tagcggaaag gatcagcgca atacgcgtcc
    15301 agaacggcgg ctttttcttg ccgtctttct tttcacgctc cgctggggtc aggtggatac
    15361 gggcgcgttt cttggtgccg ctccagtagc gacgcagcaa gaaaatcaga ccgccagcaa
    15421 acaccaggcc gacaataggg gaaacgatca gagaaccgaa aatacttaat actttcggaa
    15481 tattgagtgc atccaccact gacgtcccgg tcatcaacgc attggttaaa ccaatcccga
    15541 tgatcgcgcc aatcagcgta tgggagctgg atgcaggtaa accaaagtac caggtaccca
    15601 ggttccagat aatcgccgcc agcaacatag agaacaccat ggcaaggcca tgagacgatc
    15661 ccatattaag cagcagatcc gtcggcagca tatgcacaat ggcataggca acactcagac
    15721 cacccagcaa aacacccaaa aagttgaaca ccgctgccat aaccacggcg agctgagaac
    15781 gcatcgcgcg ggtatagata acggttgcca cggcgttggc tgtgtcatgg aaaccattga
    15841 tggcttcgta gaacagcaca aaagccagtg caagcaataa taacagcccg gtatgcaaat
    15901 ccaggccagc aaacaaatgt agcataggac gttacgccat tttgaggaca tgaacgcggc
    15961 gcattatcag tgactttcgc ggcgcgggca aagtgaaata tagacttttt ttgatttgcc
    16021 tcctgtatgg atttcactca aaaaataatt atcttatata attcaggcaa atacttcctt
    16081 ttagtaatat tgatgctggt gcgaccactg aggaatcttt acaattcacg cccgtttttt
    16141 ctaagaggag cgcaacgtgg aaaggtttga tgccattatt ataggcgctg gtgcggcggg
    16201 tatgttctgt tctgcgctgg caggtcaggc aggacgccgg gttctgctga tcgataatgg
    16261 taaaaaacca gggcgcaaaa tccttatgtc tggcggtggg cgctgcaact ttaccaacct
    16321 ttatgtcgaa ccgggcgctt atctgagcca gaatccgcat ttttgtaagt ctgcactcgc
    16381 gcgttttacc cagtgggatt tcattgatct ggtcaataaa cacggcatcg cctggcacga
    16441 gaaaacgtta gggcaactct tctgcgatga ctccgctcag cagattgtcg acatgctggt
    16501 ggatgagtgc gagaagggca atgtgacctt cagattgcgt agcgaagtgc tgagcgtgga
    16561 gaaggatgat acaggtttca cgcttgaact gaacggcatg actgtcggtt gcgaaaagct
    16621 ggtcatcgcg accggtggac tgtcaatgcc ggggctgggc gcatcgccgt ttggttataa
    16681 gattgccgaa caatttggcc tcaacgtgct gtcgacccgc gcgggtctgg tgccattcac
    16741 tctgcataaa ccgttgctcg aagagttaca ggtgctggcg ggcgtggcgg tgccttccgt
    16801 gattaccgct gaaaatggca ccgttttccg agagaactta ctcttcaccc atcgcggctt
    16861 gtctggaccg gcggtgttgc aaatttccag ctactggcaa ccgggggaat ttgtcagcat
    16921 caatctgcta ccggatgtgg atctcgaaac cttcctgaat gagcagcgta acgcacatcc
    16981 gaatcagagc ctgaaaaaca cactggcggt tcatctaccg aagcggttgg ttgaacgctt
    17041 acagcaactc gggcaaatcc cggatgtttc gctaaaacag ctcaacgtgc gtgaccaaca
    17101 ggcactgatt agcacattga ccgactggcg cgtacaaccc aacggcactg aaggctatcg
    17161 cactgccgaa gtgacgctcg gcggcgtgga caccaacgaa ctctcttcac ggacgatgga
    17221 agcgcgcaaa gtgcctgggc tgtacttcat cggcgaagtg atggacgtca ccggctggct
    17281 ggggggctat aacttccagt gggcgtggtc gagtgcgtgg gcttgtgcgc aggatttgat
    17341 tgtagagaag tcgtcctgag ctatttccta aagatgaaaa ttattagagt cgcattaaaa
    17401 atgagctaat tttgatagtg gctatcttgt gattattttc taatgagccc gtgaactgaa
    17461 cccccatgct taatataagg tggaaggaaa ggtgattgaa aactcaccca gtgggctgat
    17521 ctttataaaa tatgaacagg ttgaaaaaaa aacagtaact ttgctgtttt ttatacttaa
    17581 ccactatgca ttaatgctgc gttatttcat tctacctaag aaaaaccagc gttacgcaaa
    17641 tggtcaacgc tggttttatc cggtacgttg caattatttt ttagcagaac ccgcttctaa
    17701 tattgaaacg attgagaaca acgtaaagca taccgctccc aaaccgacca gaacccgcga
    17761 aggaataaaa tatcccatat cggtctgcgc catttccgca agaaacgatg ccaggaaaag
    17821 gcaaaacagg caggtgaaga cgggaatcat cggtattctg ttggctaacg aacaggtacg
    17881 tctccagacc agtgccagta gccagacttt tgagaatatg ctgtaacaga tcattccgag
    17941 gcaaatgaga ataatgccgg gagccagtcg ggcgcttgca tcggagctga ctaagacgta
    18001 tatgccctgc agcaccgtga ttgagccgag aaaaataacc caataacacc acagccagtg
    18061 ctctttcgtt gaaaacgtat tacgtgtttg atggacgatt gtggcaacaa ggccaattag
    18121 acaggcacag attgcggtta acccgagcaa tacgtgaccg gcgacataat gcggagttat
    18181 gtcggcacta cgtaacagcg taatagacca aatgaaaccg agcagggtga gtaacacggg
    18241 aacggcaatg aggcaattac cgattaatga agaataagcc tgtaccggag ttccgtcgct
    18301 cttgctcccc gctgcgtttt taggaatgag cagaaagtga ccggatgacg ctgccactgt
    18361 cgatacacag gcggcaatca taccaacgcc gaaaataaca tggccggcga caaactcgtc
    18421 tgccatcaca tcattacctg ctaacagtgt ccaaccccat atcatggtga taattgaccc
    18481 cgcatagcca ataatgggga acaatgtatt gtaaaacgta ttaacgccgc gcgtgagctg
    18541 cgaaatgata ataaatgcgg tagtgaataa tgccaggcat atggctgcca gagaaatcag
    18601 cacatgaccc gcgacgaaat attcgctttg ccctgaactt tccagcacaa agccacctaa
    18661 cgcgatacaa atcaagccca taattaaagg gattaatttg aaaagccacc cgatatatat
    18721 gttcacttta aactcctcag gtatccctgc taatattgaa atgtgcatta acgataattt
    18781 cagcctgtta atacaatgac gagatatcat taaagacaaa tgcatagcat aactatagcg
    18841 gggtttact
//
LOCUS       DAPGOF010000091        18536 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.091, whole genome shotgun sequence.
ACCESSION   DAPGOF010000091 DAPGOF010000000
VERSION     DAPGOF010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.091"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(18..641)
                     /gene="rsmG"
                     /locus_tag="RHV13_002787"
     CDS             complement(18..641)
                     /gene="rsmG"
                     /locus_tag="RHV13_002787"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDV1242307.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(705..2594)
                     /gene="mnmG"
                     /locus_tag="RHV13_002788"
     CDS             complement(705..2594)
                     /gene="mnmG"
                     /locus_tag="RHV13_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDV1242308.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(2973..3416)
                     /gene="mioC"
                     /locus_tag="RHV13_002789"
     CDS             complement(2973..3416)
                     /gene="mioC"
                     /locus_tag="RHV13_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HDV1242309.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(3506..3964)
                     /gene="asnC"
                     /locus_tag="RHV13_002790"
     CDS             complement(3506..3964)
                     /gene="asnC"
                     /locus_tag="RHV13_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HDV1242310.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4116..5108
                     /gene="asnA"
                     /locus_tag="RHV13_002791"
     CDS             4116..5108
                     /gene="asnA"
                     /locus_tag="RHV13_002791"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HDV1242311.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLEL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(5113..6564)
                     /gene="viaA"
                     /locus_tag="RHV13_002792"
     CDS             complement(5113..6564)
                     /gene="viaA"
                     /locus_tag="RHV13_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HDV1242312.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRNRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARLLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(6558..8054)
                     /gene="ravA"
                     /locus_tag="RHV13_002793"
     CDS             complement(6558..8054)
                     /gene="ravA"
                     /locus_tag="RHV13_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HDV1242313.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            8277..10145
                     /gene="kup"
                     /locus_tag="RHV13_002794"
     CDS             8277..10145
                     /gene="kup"
                     /locus_tag="RHV13_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HDV1242314.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            10312..10731
                     /gene="rbsD"
                     /locus_tag="RHV13_002795"
     CDS             10312..10731
                     /gene="rbsD"
                     /locus_tag="RHV13_002795"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDV1242315.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            10739..12244
                     /gene="rbsA"
                     /locus_tag="RHV13_002796"
     CDS             10739..12244
                     /gene="rbsA"
                     /locus_tag="RHV13_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HDV1242316.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            12249..13214
                     /gene="rbsC"
                     /locus_tag="RHV13_002797"
     CDS             12249..13214
                     /gene="rbsC"
                     /locus_tag="RHV13_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HDV1242317.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            13239..14129
                     /gene="rbsB"
                     /locus_tag="RHV13_002798"
     CDS             13239..14129
                     /gene="rbsB"
                     /locus_tag="RHV13_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HDV1242318.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            14255..15184
                     /gene="rbsK"
                     /locus_tag="RHV13_002799"
     CDS             14255..15184
                     /gene="rbsK"
                     /locus_tag="RHV13_002799"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDV1242319.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            15188..16180
                     /gene="rbsR"
                     /locus_tag="RHV13_002800"
     CDS             15188..16180
                     /gene="rbsR"
                     /locus_tag="RHV13_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HDV1242320.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(16146..17573)
                     /gene="mdtD"
                     /locus_tag="RHV13_002801"
     CDS             complement(16146..17573)
                     /gene="mdtD"
                     /locus_tag="RHV13_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HDV1242321.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(17596..18288)
                     /locus_tag="RHV13_002802"
     CDS             complement(17596..18288)
                     /locus_tag="RHV13_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDV1242322.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VINHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRREHWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIVQSNGDAAF
                     KACQALLRSPDK"
BASE COUNT         4465 a         4592 c         4840 g         4639 t
ORIGIN      
        1 gatttttttg ataaaaatta aattttattt gctttaatca ccaccagatg acgttcgcca
       61 tccagggctg gaacctgaag tttaaccact gattcgacct gatattcttc gggcaacaaa
      121 gcgatttcat cttccggcat ttgccctttc agcgcgtaga aacggccttg ctcaccagga
      181 agatggtggc accagctcac catatcgttc agagaggcaa aagcgcggct aattacgcca
      241 tcaaatggcg gctctgaagg aaactcttct accctgctct gtactggttc aatattctcc
      301 agtttaagct catgttgcac ctgacgaagg aaacgcacgc gtttaccaag gctatccaac
      361 agagtgaaat gggcttcagg acgcacgata gagagtggaa tgcctggcag tcctggtccg
      421 gtgccgacat cgataaaccg ttcaccttgc agatacggtg ccaccacaat gctatcgaga
      481 atatggcgta ccagcatctc attaggatcg cggaccgaag tcaggttgta cgctttgttc
      541 catttatgca gcatattcac gtaggcaata agctggtttt tctggtgatc ggtaagcgaa
      601 atacctgcgt ctttcagcag taaggagagt ttgttgagca cggtgattac ctgttcttga
      661 tgcgttgcct ggtaagcggg tgcttaccag gcatttttaa tgcgttatgc gctacgacgc
      721 agcataccct gttttttcag ccacaccagc agaatggaga tggccgcagg cgtgacgcca
      781 gaaatacgcg aagcctggcc gatagaggcc ggtttgtgat cgttaagttt ggcgatcact
      841 tcgttagaaa gaccggatac ctggcggtaa tccagtgtcg cgggtagcag ggtgttctcg
      901 ttacgcagct gcttttcgat ctcatcttgc tggcgcgcga tataaccttc gtatttaacc
      961 tgaatctcaa cctgttccgc cgcctgttcg tctgtcaacg caggggcaaa cggcgtcagc
     1021 gtggttaatt tttcataagt catttccgga cgacgcagca gatcttcacc actggcttca
     1081 cgggaaagcg gcgcagtcag gtgagcattc acttcggctg cagcttccgc cgacggggtt
     1141 acccaggtcg atttcagacg ctgacgctca cgctcgatat tctcaagttt ctcgttaaag
     1201 cgcgcccaac gttcgtcatc caccaggccc agttcacgac cgatttcagt caaacgcaga
     1261 tccgcattat cttcgcgtag catcagacga tattctgcgc gcgaagtaaa catacgatac
     1321 ggttctttgg ttcctaaagt gcacaggtca tcaaccagta cgccgagata cgcctgagaa
     1381 cgcgccggag cccaaccttc tttgtcagca gacagacggg cagcgttaag accggccagc
     1441 aaaccttgcg cagcggcttc ttcgtaaccg gtagtgccgt taatctgacc agcaaagaac
     1501 agcccctgga taaacttgct ctccagcgtc ggtttcaggt cgcgtggatc gaagaagtca
     1561 tactcaatgg cataacccgg acgcacgatc ttcgcgtttt ccatcccctg catggagcgg
     1621 acgatttgca tctgcacatc gaacggcagg ctggtggaga taccgttcgg ataaatttcg
     1681 ttagaggtca gcccttccgg ttcaaggaag atctgatgct gatttctgtc ggcgaagcgc
     1741 atgactttgt cttcgatcga cgggcagtag cgtgggccga caccttcgat cacccctgcg
     1801 tacattgggc tacgatcgag gttactgcgg atcacatcat gggttttctc gttggtatgg
     1861 gtgatataac acggcacctg ctgtggatgc tgggacgcat tgcccataaa agagaatacc
     1921 ggcattgggt tatcgccatg ctgttgcgca agcacgctaa agtcgatggt tcgagcatca
     1981 atacgcggtg gtgtcccggt tttcagacga ccaacgcgta gcggaagttc acgcaaacgg
     2041 cgagaaagcg gaatggacgg cggatcacca gcacggccac cgctgtagtt atccagaccg
     2101 atatgaattt taccgtcgag gaacgtccca acggtgagca cgacggcttt ggcacggaac
     2161 ttcagtccca tttgggtaac agcaccgacc acacgatcgt tttcgacaat aagatcttca
     2221 accgcctgct ggaagatcat caggttcggt tggttctcca gcgccgtacg taccgcctga
     2281 cggtagagca cacgatccgc ctgagctcgg gtagcgcgaa ctgccggtcc cttgcttgcg
     2341 tttagtatcc taaactggat acccgcctga tcgatcgctt tcgccatcag accgccgagt
     2401 gcatccactt cttttaccag atgtcccttc ccaataccgc cgatcgccgg gttgcagctc
     2461 atctgcccca gagtgtcgat attgtgtgtc aaaagcagag tctgttgacc catacgcgcc
     2521 gcagccatcg cggcctcggt gcctgcatga cccccgccaa tgatgatgac gtcaaaagga
     2581 tccggataaa acatggtgat tgcctcgcat aacgcggtat gaaaatggat tgaagcccgg
     2641 gccgtggatt ctactcaact ttgtcggctt gagaaagacc tgggatcctg ggtattaaaa
     2701 agaagatcta tttatttaga gatctgttct attgtgatct cttattagga tcgcactgcc
     2761 ctgtggataa caaggatccg gcttttaaga tcaacaacct ggaaaggatc attaactgtg
     2821 aatgaacggt gatcctggac cgtataagct gggatcagaa tgaggggtta tacacaactc
     2881 aaaaactgaa caacggttgt tctttggata actaccggtt gatccaagct tcctgacaga
     2941 gttatccaca gtagatcgca cgatctgtat acttatttga gtaaattaat ccacgatccc
     3001 agccattctt ctgccggatc ttccggaatg tcgtgatcaa gaatgttgat cttcagtgtt
     3061 tcgcccgtct gttttgcacc ggaatttttg agttcagcct cgagtttatc gatagcccca
     3121 caaaaggtgt catattcacg actaccaata ccgattgcgc caaagcggac cgcagaaaga
     3181 tcgggcttct gttcctgcaa tgcttcatag aaaggagaaa ggttgtccgg aatatctccg
     3241 gcaccgtggg tggagctgat aaccagccag atccccgagg cagataaatc ttccaacagc
     3301 ggaccgtgca gcgtttcggt ggtaaaaccc gcctcttcca gcttttcagc caggtgttct
     3361 gctacatatt cggcaccgcc gagggtgctg ccgctgataa gagtgatatc tgccataaac
     3421 cgccaccttt attaagagtg gcgtattgta cgctgtgaac gcgttgggat ctacctgtgg
     3481 aaaagtatgg gattaaaaaa gccgatcagg gcttgatggt acgcatgatc gggttctgca
     3541 ggacgatcag tgtctcggtg gactgaattt catcaattgt ttggatcttg ttgataagta
     3601 catgctggag agcatcgatc gaacggcaca tcacttttat aaagatgctg tagtggccgg
     3661 tggtgtagta cgcctcggtc acttcatcga ggctttccag ctttgccagc gcggaagggt
     3721 agtctttggc gctctttaat ataatgccga taaagcagcc tacgtcataa ccaagctgct
     3781 tcgggctgac atcaatacgc gccccggtaa tgatccccgc ctgcttcatt ttctctactc
     3841 gaacgtgaat agtccccgga ctgacgccaa attgtttcgc cagttcggcg taagcggtgc
     3901 gcgcattgcc cattaatgct tccaggatgc cacggtccag attgtcgatc agataatttt
     3961 ccataggatt ttcttatgcg gattgatgat tcattctatt ttagccttct tttttaatga
     4021 atcaaaagtg agttaggctt tttattgaat gattattgca tgtgtgtcgg tttttgttgc
     4081 ttaatcataa gcaacaggac gcaggagtat aaaaaatgaa aaccgcttac attgccaaac
     4141 aacgccaaat tagcttcgtg aaatctcact tttctcgtca actggaagaa cgtctggggc
     4201 tgatcgaagt ccaggcgccg attcttagcc gtgtggggga tggcacgcag gataacttgt
     4261 cgggctgtga aaaagcggta caggtaaaag tgaaagctct gcctgatgcc cagttcgaag
     4321 tggttcattc actggcgaag tggaaacgtc agaccttagg gcaacacgac ttcagcgcgg
     4381 gcgaaggact gtacacgcac atgaaagccc ttcgccccga tgaagaccgt ctttctccgt
     4441 tacactcggt ctatgttgac cagtgggact gggaacgcgt aatgggcgac ggtgagcgcc
     4501 aattctcgac tctgaaaagc acggtagagg cgatctgggc gggaattaaa gcaaccgaag
     4561 ctgcggttag cgaagagttt ggcctggcac cgttcctgcc ggatcagatc cacttcgtac
     4621 acagccagga gttactgtct cgttatccgg atctcgatgc caaagggcgt gagcgggcga
     4681 ttgcgaaaga tcttggcgcg gtattcctcg tcgggattgg cggcaagttg agtgatggtc
     4741 atcgtcacga cgtgcgcgca ccggattatg atgactggag caccccgtca gagctgggcc
     4801 atgcgggcct gaacggcgat attctggtgt ggaacccggt actggaagat gcgtttgagc
     4861 tttcttccat ggggatccgc gtggatgccg acacgctgaa gcatcagctg gcgctgaccg
     4921 gtgacgaaga tcgcctggag ctggagtggc atcaggcgct gctgcgcggt gaaatgccgc
     4981 agaccatcgg cggcggtatc ggccagtctc gtttgaccat gctgctgctg caactgccac
     5041 atataggcca ggttcagtgt ggagtatggc cagctgctgt tcgcgagagc gtcccttctc
     5101 tgctgtaata atttatcgcc gccagcgtct gagcaggcgg cttcgcatcc cggtatcaaa
     5161 gcgccagata tgatcgaaaa tgcgcatgat gccgggtttg ccgtgtgccg acatcgccac
     5221 ggcatgaaag cgatgctgat gtacccgctg cagctctttc actttactcg tcacgtcgtc
     5281 aggcaaccgc tgagcgataa aatcagaaat caccaccgca tcggcatcaa accattccct
     5341 gctttgcaag cgttccataa tggcgcgaaa acaactggca agatcggtgc cgccacgaaa
     5401 ctgctggctt aaaaaacgga ttgcttgttc gatgccttgt gggcctgaaa gctcataacg
     5461 gacgatctcg gtggaaaata gcataatata gcagcgccgg ttttcagcga gagcaatgcg
     5521 catcaaggcc aggcagaacg ctttcgcaca ctgttcatta aagccgccca ttgagccgga
     5581 agtatccaca cagacaataa acggcccgcg cggctgttcg tcgtaatctt tatgcaccac
     5641 cggacgttcg attacttttt cacgccacga ctcaccgtgc aggcgatagg tgagcaactg
     5701 tttttccacc agccgacggt aaaactcata ctccagttcc gttatcccta gtgtcgccag
     5761 ttctggcggc aggagacgta aaatatcatc gctttgttgc agaccatcaa cctgctcagg
     5821 aacagttgcc ggttcgcgca ccatggtgcg gaaggtttcc atctgcgcat cgttgcgcgg
     5881 tattgatttg gcttcccggg aacgccccag ctgctctgcc aggcgtttca gttccggctg
     5941 ttcgttaaga aattcaccgt atttcacaat caactgatag tcgccgcgtt taagctgacc
     6001 ggcgctcata tcccacagac gaccagctgc ggtattgtta tctgcgagaa tcggttcaag
     6061 ttgtccgctc agcgtcatgc gttcctgaac ttcacttaac agttgttcgc gttcttcttc
     6121 taatagctgt tgatttaacg tcgttgcttg cacgatcaga cttaaacgcc agcgctggag
     6181 aaaaagcgtg tgtaacgctg aagtgatcgt gctgttagca tcaaccaaca gtcgggcttg
     6241 ttctgcccat ggggaattca gacgatgcag taagtccagg atctgtggta gctgcacaat
     6301 aaactgcggc gtggagagga gctggctttg ctgatagcac atcacctctt cggtgagttc
     6361 cggcgggact cgggcatctt tcagccgatt acgcagcgcc tcacgccagc ggggaacatc
     6421 atcagtgatt gccgccttca gtcgtgggaa tttttcaaag aagaccgcca gctgcggtga
     6481 ggccagcagc gcgatgatca tctcttcgat caatccctct tcgctgacgg ccagcatcac
     6541 attaagcgta tccagcgtta gcattgttgt gcctggcgaa tctgtgcgcc gacatcctgt
     6601 aggctggctt caatgcgacc taaccagtcg ccagggataa acagacattt ttgctgttcg
     6661 ctgaataaag cgtgttgctt gcgccagtca ctttcaagct cttccagttg ttgttttatt
     6721 tcacctggca gaccttcagc cgatgaaccg ggaagagcca gcgtactgcc ttgtaaactt
     6781 acatcgcgta caacaagatg ttgggcgcta tccacttcca gattcagttt ctgggcaaag
     6841 ccgataccgt tcagtttgcc gcgaatttca ccacctttac tcagccactg ttccagcgcg
     6901 ctacgctcaa aggagatatg aaccacttcc atatcatgca gttttaacgg tttttgcagc
     6961 agcagagtca gagtggaagc agtaacgtta acagggagtt gatactgctg acgacggctg
     7021 aaaatgccgc ccagacgaat taccgttaag gctgttttat cactttgttg ctgctggagt
     7081 tgcaggtgac gttgcacaat cgcgccaagg cgagtcaaca tcccttgctg ttgccaggcg
     7141 tgaccggtca tcaatacatc aatttgttgt tgtatcaaat tcaggctttg cgcgtcatac
     7201 cacaggcaat ctttcagcaa aatgagatct accggggcaa cagcactgcg accgctaaaa
     7261 aaggcgctgg cctgcaataa gcggatcgct tttttccagc gacgatccga gacataaggc
     7321 gcatccggta atttatccag ttgctggcgc agcataaaaa tcagctcaaa tacatgatcg
     7381 ggcagcgtaa tttcaccaat ctctttctgc cagcgttcat attcttcatc tgtgacctgc
     7441 aaggcatcag gaaccggatt gtcgttttca tcctgttgac tggtcagcat ggagcggaaa
     7501 ttcgctttat cctgcacttt atctaaccac agacgaatca gcatgcggtc atataacgct
     7561 tccagactgc tgtctgcttc cggcagctcg ttggaggccg ccaccagtaa acgcatcggg
     7621 attttttcta cgtgtgcacc gttgcggaac tggcgctcgt taatggcggt gagcaaggta
     7681 ttaagaattg ccgggcccgc tttccagatc tcatccagaa agacaatttc tgcttccggc
     7741 aggtaaccgc tggttaaacg ttcatagcgc ccttcatctt ttagcgcctg aatagaaagg
     7801 ggaccaaaaa cttcttccgg cgtggagaag cgggtcatca gatattcaaa cgcgcgggca
     7861 ttctgaaagg cgaattttaa gcgccgggcg atcaaacttt tggcaatacc tggcgggcca
     7921 aggaggaaca cactttcacc acttaatgcc gctaataaac acaagcggat ggcgtggcta
     7981 cgttcataaa gccccttttc caacgaactg ctcaggcggg aaattctttc cgctaataaa
     8041 tgagggtgag ccataatgaa gtggcgtcct ttcgtcaaaa gttctgcgta aattgcgagt
     8101 atagacgttt cttgctggtg gctaaaatag tctcaaaggg ggggtatttt tctttgagcc
     8161 aggttaatgt gaccgcattt aggagtacga ttttgccgtt aatcgtgcat actgtgcgct
     8221 tttttgtggg ccaagggact aagcacacat ttcatatttc aacgaaagac tagtctatga
     8281 gcactgataa taagcaatca ttgcccgcga ttaccctcgc ggcgattgga gttgtctacg
     8341 gcgatattgg taccagcccg ttatatacac ttcgtgaatg tttgtccggc cagtttggtt
     8401 ttggcgttga acgcgatgcc gtgtttggct ttttatcgct gatcttctgg ctgctaatct
     8461 ttgtggtttc cattaaatat ctcaccttcg tgatgcgggc agataacgcc ggtgaggggg
     8521 ggatcctgac gttgatgtcg cttgccgggc gtaatacgtc ggcgcgaacc acatcaatgc
     8581 tggtgattat ggggctaatc ggcggcagtt ttttctatgg tgaagtcgtc ataacacccg
     8641 ctatttcggt gatgtcagcc attgaaggtc tggaaatcgt cgccccgcag ctggatacct
     8701 ggatagttcc cctctcaatt atcgttctca ctttattatt tatgattcaa aagcatggca
     8761 cggctatggt cggtaagctg tttgcgccga tcatgctgac ctggtttttg attctggcag
     8821 gactggggtt acgtagcatt attgctaacc cggaagtgct gcatgcgctg aatccgatgt
     8881 gggcggtgca tttcttcctt gaatacaaaa cggtttcttt tattgcatta ggggcagtgg
     8941 tgctgtcgat tacgggggtc gaggcgctgt atgctgatat gggacacttt ggtaagttcc
     9001 ctattcgtct ggcgtggttc actgtcgtat taccttcctt aactcttaat tacttcggcc
     9061 agggagcgct gttgttaaag aacccggaag cgattaagaa cccgttcttc ctgttggcac
     9121 cggactgggc gctgatcccg ctgctgatca tcgccgcact ggcgacggta atcgcctcgc
     9181 aggcggttat ctctggcgtc ttttcattga cgcgtcaggc ggtacgtctg ggatatttgt
     9241 cgccgatgcg cattattcac acctccgaaa tggagtcagg gcaaatctat attccgtttg
     9301 tgaactggat gctctatgtc gcggtcgtga ttgtgattgt cagctttgag cactccagca
     9361 acctggcggc ggcgtacggg attgcggtga ccggaaccat ggtgctgacg tctattctct
     9421 cgactaccgt ggcacgtcag aactggcact ggaataagta ttttgttgcg ctgatcctga
     9481 ttgctttcct ttgtgtcgat attccattgt tcaccgctaa cctcgataaa ctgctctccg
     9541 gcggctggtt gccattgagc ctcggtactg tgatgtttat cgtgatgacc acctggaaga
     9601 gcgagcgttt ccgcttgctg cggcggatgc atgaacatgg taactctctg gaagcgatga
     9661 ttgcttcgct ggagaaatca ccgcctgttc gcgtgcccgg gaccgcggtg tatatgtcgc
     9721 gtgcaatcaa cgtcattccc tttgcgctaa tgcataacct taaacataac aaggtattgc
     9781 atgagcgggt gattctgtta actctgcgca ccgaagacgc gccatatgtc cataacgtcc
     9841 gtcgggtaca gattgaacaa ctgtcgccca ctttctggcg cgtggtggca agttatggtt
     9901 ggcgagaaac gccaaacgtg gaagaagttt tccaccgctg cggtctggaa ggattaagtt
     9961 gccggatgat ggaaacctcc ttctttatgt cgcatgagtc gttgatcctc ggcaaacgcc
    10021 cgtggtattt gcgtctgcgc ggcaagctgt acttgctgct gcaacgtaat gcgctgcgtg
    10081 caccagatca atttgaaatc ccgccaaaca gggttatcga actgggtact caggtcgaaa
    10141 tctaacgcca gacgcctcct ttcttcgtaa gggggcgttt ttgttttcat ggttaatcac
    10201 catgtaaaac gtttcgaggt tgatcacatt tccgtaacgt cacgatggtt ttcccaactc
    10261 agtcaggatt aaactgcggg tcagcgaaac gtttcgctga tggagaaaaa aatgaaaaaa
    10321 ggcaccgttc ttaattctga tatttcatcg gtgatctccc gtctgggaca taccgatacg
    10381 ctggtggtgt gtgatgctgg tttacccatc cccaaaagta caacgcgtat cgatatggca
    10441 ttaacccagg gtgtaccttc ttttatgcag gtgctgggcg tcgtcacaaa tgaaatgcag
    10501 gtcgaggctg ccattatcgc ggaagagatc aaacaacata atccgcaact ccacgaaacg
    10561 ttgctcactc accttgagca gctgcaaaaa caccagggaa ataccattga aattcgttac
    10621 accacgcatg aacaattcaa acaacaaacc gcagaaagtc aggcggtaat tcgcagcgga
    10681 gaatgttctc cgtatgcgaa tatcattctc tgtgctggcg tgacgttctg aggccgtcat
    10741 ggaagcatta cttcagctta aaggcatcga taaagccttc ccgggcgtaa aagccctctc
    10801 gggcgcagcg ttaaatgtct atccgggccg cgtgatggcg ctggtgggcg aaaacggcgc
    10861 gggtaaatcc accatgatga aagtgcttac tggtatctat actcgcgatg ccggcacgct
    10921 tttatggctg gggaaagaaa cgacatttac cgggccgaaa tcttcccagg aagccgggat
    10981 tgggattatc catcaggaac tgaacctgat cccgcagttg accattgccg aaaacatttt
    11041 cctcggtcgt gagtttgtta atcgctttgg caaaattgac tggaaaacca tgtatgccga
    11101 agcggataaa ttgctggcta aacttaacct gcgctttaaa agcgacaagc tggtgggcga
    11161 tctttccatc ggtgaccagc aaatggttga aatcgccaaa gtgctgagct ttgaatcgaa
    11221 agtcatcatt atggatgaac cgaccgatgc gctgaccgat accgaaaccg aatccttgtt
    11281 ccgcgtcatc cgcgagctga aatcgcaagg ccgcggtatt gtctatatct cccaccgcat
    11341 gaaagaaatc ttcgagattt gcgatgacgt taccgttttt cgtgatgggc aatttattgc
    11401 tgagcgcgaa gtggcatcac tgaccgaaga ttcgctgatt gagatgatgg tgggccgcaa
    11461 gctggaagat caatatccac acctggacaa agcgccagga gatatccgcc tgaaagtcga
    11521 taatctctgc ggacctggcg ttaacgatgt ctcttttact ttacgcaaag gcgaaattct
    11581 tggcgtctct ggtttgatgg gcgcaggtcg taccgaactg atgaaagtgc tctacggcgc
    11641 actaccgcgc accagcggtt acgtcaccct ggatgggcat gaagtcgtta cccgttcacc
    11701 gcaggatggc ctggcaaacg gcattgtgta tatctccgaa gaccgtaaac gtgacggttt
    11761 agtgttgggc atgtcagtaa aagagaacat gtcgctgacc gcgctgcgct acttcagccg
    11821 cgctggcggc agtttgaagc atgccgatga acagcaggct gtgagtgatt tcattcgtct
    11881 gtttaatgtg aaaacgccgt cgatggaaca ggcaattggt ctgctttccg gtggcaatca
    11941 gcaaaaagtg gcgattgccc gcggtctgat gacacgcccc aaagtgttga tccttgatga
    12001 acctacccgt ggcgtagatg tcggcgcgaa aaaagagatc tatcaactga ttaaccagtt
    12061 caaagccgat ggcttgagca tcattctggt gtcatcggag atgccagaag tattaggcat
    12121 gagcgatcgc atcatcgtca tgcatgaagg gcatctcagc ggggaattta ctcgtgagca
    12181 ggccacccag gaagtgttaa tggctgccgc tgtgggcaag cttaatcgcg tgaatcagga
    12241 gtaaaaaaat gacaacccag actgtctctg gtcgccgtta tttcacgaaa gcgtggctga
    12301 tggagcagaa atcgcttatc gctctgctgg tgctgatcgc gattgtctcg acgttaagtc
    12361 cgaacttttt caccatcaat aacttattca atattctcca gcaaacttcg gtgaacgcca
    12421 ttatggcggt cgggatgacg ctggtgatcc tgacgtcggg catcgacttg tcggtaggtt
    12481 ctctgttggc gctaaccggc gcagttgctg catctatcgt cggcattgaa gtcaatgcgc
    12541 tggtggctgt cgctgctgct ctcgcgttag gtgccgcaat tggtgcggta accggggtga
    12601 ttgtagcgaa aggtcgcgtt caggcgttta tcgctacgct ggttatgatg cttttactgc
    12661 gcggcgtgac catggtttat accaacggta gcccagtgaa taccggcttt actgagaacg
    12721 ccgatctgtt tggctggttt ggtattggtc gtccgctggg cgtaccgacg ccagtctgga
    12781 tcatggggat tgtcttcctc gcggcctggt acatgctgca tcacacgcgt ctggggcgtt
    12841 acatctacgc gctgggcggc aacgaagcgg caacgcgtct ttctggtatc aacgtcaata
    12901 aaatcaaaat catcgtctat tctctttgtg gtctgctggc atcgctggcc gggatcattg
    12961 aagtggcgcg tctctcctcc gcacaaccca cggctgggac tggctatgag ctggatgcta
    13021 ttgctgcggt ggttctgggc ggtacgagtc tggcgggcgg aaaaggtcgc attgttggga
    13081 cgttgatcgg cgcattaatt cttggcttcc ttaataatgg attgaatttg ttaggtgttt
    13141 cctcctatta ccagatgatc gtcaaagcgg tggtgatttt gctggcggtg ctggtagaca
    13201 acaaaaagca gtaataacga ctacaggaca tcttgaatat gaacatgaaa aaactggcta
    13261 ccctggtttc cgctgttgcg ctaagcgcca ccgtcagtgc gaatgcgatg gcaaaagata
    13321 ccatcgcgct ggtggtctcc acgcttaaca acccgttctt tgtatcgctg aaagatggcg
    13381 cgcagaaaga ggcggataag cttggctata acctggtggt gctggactcc cagaacaacc
    13441 cggcgaaaga gctggcgaac gtgcaggact taaccgttcg cggcaccaaa attctgctga
    13501 ttaacccgac cgactccgac gcagtaggta atgctgtgaa gatggctaac caggcgaaca
    13561 tcccggttat cactcttgac cgccaggcaa cgaaaggtga agtggtgagc cacattgctt
    13621 ctgataacgt actgggtggc aaaatcgccg gtgattacat cgcgaagaaa gcgggtgaag
    13681 gtgccaaagt tatcgagctg caaggcattg ctggtacatc cgcagcccgt gaacgtggcg
    13741 aaggcttcca gcaggccgtt gctgctcaca agtttaatgt tcttgccagc cagccagcag
    13801 attttgatcg cactaaaggt ttgaacgtaa tgcagaacct gttgaccgct catccggatg
    13861 ttcaggctgt attcgcgcag aatgatgaaa tggcgctggg cgcgctgcgc gcactgcaaa
    13921 ctgccggtaa atcggatgtg atggtcgtcg gatttgacgg tacaccggat ggcgaaaaag
    13981 cggtgaatga tggcaaacta gcagcgacta tcgctcagct acccgatcag attggcgcga
    14041 aaggcgtcga aaccgcagat aaagtgctga aaggcgagaa agttcaggct aagtatccgg
    14101 ttgatctgaa actggttgtt aagcagtagt tttaatcagg ttgtatgacc tgatggtgac
    14161 ataaatacgt catcgacaga tgaacgtgta atataaagaa aagcagggca cgcgccaccc
    14221 taacacggtg gcgcatttta tggacatccc gaatatgcaa aacgcaggca gcctcgttgt
    14281 tcttggcagc attaatgctg accacattct taatcttcaa tcttttccta ctccaggcga
    14341 aaccgtaacc ggtaaccact atcaggttgc atttggcggc aaaggcgcga atcaggctgt
    14401 ggctgctggg cgtagcggtg cgaatatcgc gtttattgcc tgtactggcg atgacagcat
    14461 tggcgagagc gttcgccagc agctcgccac tgataacatc gatattactc cggtcagcgt
    14521 gatcaaaggc gaatcaacag gtgtggcgct gatttttgtt aatggcgaag gtgagaatgt
    14581 catcggtatt catgccggcg ctaatgctgc cctttccccg gcgctggtgg aagcgcaacg
    14641 tgagcgtatt gccaacgcgt cagcattatt aatgcagctg gaatcaccac tcgaaagtgt
    14701 gatggcagcg gcgaaaatcg cccatcaaaa taagactatc gttgcgctta acccggctcc
    14761 ggctcgcgaa cttcctgacg aactgctggc gctggtggac attattacgc caaacgaaac
    14821 ggaagcagaa aagctcaccg gtattcgtgt tgaaaatgat gaagatgcag cgaaggcggc
    14881 gcaggtactg catgaaaaag gtatccgtac tgtactgatt actttaggaa gtcgtggtgt
    14941 atgggctagc gtgaatggtg aaggtcagcg cgttcctgga ttccgggtgc aggctgtcga
    15001 taccattgct gccggagata cctttaacgg cgcgttaatc acggcgttgc tggaagaaaa
    15061 accattgcca gaggcgattc ggtttgccca tgctgccgct gcgattgccg taacacgtaa
    15121 aggcgcacaa ccttccgtac cgtggcgtga agagatcgac gcatttttag acaggcagag
    15181 gtgacgcttg gctacaatga aagatgttgc ccgcctggcg ggcgtttcta cctcaacagt
    15241 ttctcacgtt atcaataaag atcgcttcgt cagtgaagcg attaccgcca aagttgaagc
    15301 ggcgattaaa gaactcaatt acgcgccatc agctctggcg cgtagcctca aactcaatca
    15361 aacacatacc attggcatgt tgattactgc cagtaccaat cctttctatt cagaactggt
    15421 gcgtggcgtt gaacgcagct gcttcgaacg cggttatagt ctcgttcttt gcaataccga
    15481 aggcgatgaa caacggatga atcgcaatct ggaaacgctg atgcaaaaac gcgttgatgg
    15541 cttgctatta ctgtgcaccg aaacgcatca gccttcgcgt gaaatcatgc aacgttatcc
    15601 gacagtgcct accgtgatga tggactgggc tccgttcgat ggagacagcg atcttattca
    15661 ggataactcg ttgctgggcg gagacttagc aacgcaatat ctgatcgata aaggtcatac
    15721 ccgtatcgcc tgtattaccg gtccgctgga taaaactccg gcgcgcctgc ggttggaagg
    15781 ttatcgggca gcgatgaaac gtgcgggtct caacattcct gatggctatg aagtcactgg
    15841 tgattttgaa tttaacggcg ggtttgacgc catgcgccaa ctgctatcac atccgctgcg
    15901 tcctcaggcc gtctttaccg gaaatgacgc tatggctgtt ggcgtttacc aggcgttata
    15961 tcaggcagag ttacaggttc cgcaggatat cgcggtgatt ggctatgacg atatcgaact
    16021 ggcaagcttt atgacgccac cattaaccac tatccaccaa ccgaaagatg aactggggga
    16081 gctggcgatt gatgtactca tccatcggat aacccagcca actcttcagc aacaacgatt
    16141 acaacttact ccgattctga tggaacgcgg ttcggcttag atttacgctg tcttttgatc
    16201 aaattattac catcggttgt tttcagaagc atgaacattg ctgctgaagc aacagtaata
    16261 atgcccattg tgataaacgt atagtggaat tgttcgacag tcgttgtgcc ttccatccct
    16321 tcataaacgc gaaggacggc cgcacttaca gcaacgccta aactaatcga cagttgctgc
    16381 gtgaccgcca gaacactgtt accgctgctg gcgttgtcat cggtcagatc ggcaagtgtg
    16441 atggtattca tcgcggtaaa ttgcgtcgac atagccatcc ctaatataaa caacggcaag
    16501 atcagcatcc atatcgccat tgccggtgat tgcaaagaga actgagcgat cattagccca
    16561 ataatcaccg tgatccccac taatgtatgg cgatagccca gacgacgtaa gacttgggta
    16621 accatcgatt ttgcaataat ggaacctaac gctgtcggtg ccatcataca gccggcaata
    16681 aacgcctgat aaccaaatcc tacctgtaac atcaatggca taaggaacgg tacacagccg
    16741 gtccccagac gggttgcaat attgcctacg ataccgatcg agaaagtgcg ggttttaaat
    16801 aaatctaatg aaattaatgg gtttggcgtg cgtcgtgcat ggagaatata gagaagcagt
    16861 aacccgatgc tggtgacaat taccgtcaag gcaatccagc tggcgacaat cttttccccg
    16921 aatagctcta ttccgcttga gaagagaaca aggctgaggc caaacaacaa aaagccagtg
    16981 atatcgaatc tgcgtcgtgc ggtggtgaaa ttgggcatat gtttgcgcgc gtaaagaagg
    17041 cctgcaatac ctatggggat attgattaaa aatatccagt gccaggttgc ccaggtgacc
    17101 agcacgccgc caagaacggg gcctaaaatt ggccccacca gacccggcat ggcgacaaaa
    17161 ttcaatactg gaagaagttc attacgagga taagcgcgca gtaaggccag tcgagcaaca
    17221 ggcatcatca ttgcgccgcc tatcccctga ataacccgga agacaaccag ctgtggtagc
    17281 gaattagaaa gtgcgcaggc cagagaaccc aatgtgaaca gactcacggc aagggtaaaa
    17341 atgcgacgcg taccgaagcg atcggctagc catccgctta ccggaataag catcgccacc
    17401 gtcagcgtat aactgatgat ggctgattgc atcgcgagag gagaacgatt aaggctatga
    17461 gcgattgcgg gtaaggcggt attcagaata gtggcatcaa gtgcctgcat gaagaaggcc
    17521 atcgccgcga tccacggcaa acccgccata ctgcgcttct ttttatcgct cattcaatgt
    17581 cctgttatcg ggttgtcact tatcaggtga gcgtagcagc gcctgacaag ctttaaatgc
    17641 cgcgtcgcca ttgctttgga caatcgcatc gacaatcgcc tggtgcagat ccagctttat
    17701 cactgtgtcg ctggtaattg acgtgaagta agtgtgataa accgaatgga atagcgaggc
    17761 gaatgaggtc aaaaacggat tggcgctcat ttcatagata tgctcatgcc aggccatatc
    17821 gacttcgatc cagtgttcac ggcgaaagtt ctcttttaat gccgccattt cggccattaa
    17881 cgtattgaga tgcgctttct gttccgcggt gccaaccgtt gctgccagta ggcaagcttg
    17941 tggctccaga cagatacgca taaccagaaa gtgattgatg acctgatgaa agttctcttc
    18001 tgtcatccac caggtaagca attcctgatc aagaaaattc cagttcgatt gtggcatgac
    18061 ccgggtacca attcgtggtc gcggtaaaac catccctttt gccgttaacg ttttgaccgc
    18121 ttcgcgtacc gctgtacgac tcactccaaa ttgctcgccc agctcaatct caccaggcaa
    18181 aatggtgccg ggttcatatt caccttttaa gatccgttgc gccagcttct cagccagaac
    18241 atacgaaagg tttttctgtg cagctaactg ttgtgcgctt aaaggcatta cttatcttcc
    18301 tttttctttt tattcctcct tagtatgcca ccaggaagtg tgattacggt tgcaaaaacg
    18361 gcaaattgct tgttttatgg cacattaacg gggcttttgc tgaaaaaatg cgcggtcaga
    18421 aaattatttt aaatttcctc ttgtcagacc ggaataactc cctataatgc gccaccactg
    18481 acacggaaca acggcaaaca cgccgccggg tcagaggggt tctcctgaga acttcg
//
LOCUS       DAPGOF010000092        18396 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.092, whole genome shotgun sequence.
ACCESSION   DAPGOF010000092 DAPGOF010000000
VERSION     DAPGOF010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.092"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            239..1138
                     /gene="yegS"
                     /locus_tag="RHV13_002803"
     CDS             239..1138
                     /gene="yegS"
                     /locus_tag="RHV13_002803"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HDV1242323.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(1220..1993)
                     /locus_tag="RHV13_002804"
     CDS             complement(1220..1993)
                     /locus_tag="RHV13_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDV1242324.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(2099..3139)
                     /gene="gatD"
                     /locus_tag="RHV13_002805"
     CDS             complement(2099..3139)
                     /gene="gatD"
                     /locus_tag="RHV13_002805"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDV1242325.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(3187..4542)
                     /locus_tag="RHV13_002806"
     CDS             complement(3187..4542)
                     /locus_tag="RHV13_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDV1242326.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHPQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            complement(4546..4830)
                     /gene="gatB"
                     /locus_tag="RHV13_002807"
     CDS             complement(4546..4830)
                     /gene="gatB"
                     /locus_tag="RHV13_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HDV1242327.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHNIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            complement(4861..5313)
                     /gene="gatA"
                     /locus_tag="RHV13_002808"
     CDS             complement(4861..5313)
                     /gene="gatA"
                     /locus_tag="RHV13_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HDV1242328.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(5323..6585)
                     /gene="gatZ"
                     /locus_tag="RHV13_002809"
     CDS             complement(5323..6585)
                     /gene="gatZ"
                     /locus_tag="RHV13_002809"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HDV1242329.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDTPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(6614..7468)
                     /gene="gatY"
                     /locus_tag="RHV13_002810"
     CDS             complement(6614..7468)
                     /gene="gatY"
                     /locus_tag="RHV13_002810"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HDV1242330.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(7777..8829)
                     /gene="fbaB"
                     /locus_tag="RHV13_002811"
     CDS             complement(7777..8829)
                     /gene="fbaB"
                     /locus_tag="RHV13_002811"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HDV1242331.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            9086..10363
                     /locus_tag="RHV13_002812"
     CDS             9086..10363
                     /locus_tag="RHV13_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HDV1242332.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            10360..11364
                     /locus_tag="RHV13_002813"
     CDS             10360..11364
                     /locus_tag="RHV13_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HDV1242333.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGINAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            11361..12326
                     /locus_tag="RHV13_002814"
     CDS             11361..12326
                     /locus_tag="RHV13_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDV1242334.1"
                     /translation="MSGVRLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAYPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(12300..13046)
                     /locus_tag="RHV13_002815"
     CDS             complement(12300..13046)
                     /locus_tag="RHV13_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDV1242335.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(13098..13916)
                     /locus_tag="RHV13_002816"
     CDS             complement(13098..13916)
                     /locus_tag="RHV13_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HDV1242336.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQAFVDGIKETP"
     gene            complement(13981..14781)
                     /gene="thiD"
                     /locus_tag="RHV13_002817"
     CDS             complement(13981..14781)
                     /gene="thiD"
                     /locus_tag="RHV13_002817"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDV1242337.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(14778..15566)
                     /gene="thiM"
                     /locus_tag="RHV13_002818"
     CDS             complement(14778..15566)
                     /gene="thiM"
                     /locus_tag="RHV13_002818"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HDV1242338.1"
                     /translation="MQVDLLSSAQSAHTLHLFHQHSPLVHCMTNDVVQTFTANTLLAI
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGVATGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGRRTVGIHGGDPLMAKVVGTGCALSAVVAACCALPGDML
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(15601..15697)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(15789..16061)
                     /gene="rcnR"
                     /locus_tag="RHV13_002819"
     CDS             complement(15789..16061)
                     /gene="rcnR"
                     /locus_tag="RHV13_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HDV1242339.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            16182..17006
                     /gene="rcnA"
                     /locus_tag="RHV13_002820"
     CDS             16182..17006
                     /gene="rcnA"
                     /locus_tag="RHV13_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HDV1242340.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTTKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEHDHEHHHHDHEHHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            17225..17563
                     /gene="rcnB"
                     /locus_tag="RHV13_002821"
     CDS             17225..17563
                     /gene="rcnB"
                     /locus_tag="RHV13_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HDV1242341.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(17645..>18396)
                     /locus_tag="RHV13_002822"
     CDS             complement(17645..>18396)
                     /locus_tag="RHV13_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HDV1242342.1"
                     /translation="LSNNQTSFTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLS
                     SASSSTRGSAAVQFLLCLLGGKLWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCK
                     PDDLLITLESIPVSQLPATGNKATVNSKKGDIILRCKNLLGQQNQTSRKMQVYLSSSD
                     LLTNSNTILKGAEDNGVGFILESNGSPVTLLNITNSSKGYTNLKEIAAKSKLTDTTVS
                     IPITASYYVYDTNKVKSGALEATALINVKYD"
BASE COUNT         4534 a         4707 c         4429 g         4726 t
ORIGIN      
        1 aatatattat attgaagatg ccactgttta gttgaatatt aggtatatgc tcttttttga
       61 aatttatcgg tggcagccgt tagtattcgc tgtccccatt gcaagctcct ggtggtaacc
      121 actgaatcct ccatacttga actgactttt tatcctccga ctttcatcct gttctgactc
      181 caccttttgt tttctgctct acactatcta cagaccaatc ataaaggcac atacgatcat
      241 ggcagaattt cccgccagct tactgattct taatggcaaa agtactgaca atctaccctt
      301 gcgcgaagca attatgctgt tgcgtgagga aggaatgacg atccatgtgc gggtcacctg
      361 ggagaaaggc gatgccgcac gatatgtaga ggaggcccgg aagttgggcg tcgcaacggt
      421 gattgccggt ggtggcgatg gcaccattaa tgaagtttct acggcgttga ttcagtgtga
      481 gggggatgac atacccgcgc tgggaatttt gccattagga accgccaatg attttgccac
      541 cagtgtaggg attcctgagg cactggataa ggcgctgaaa ctggcaattg ccggtaacgc
      601 cattgcgata gatatggcgc aggtcaacaa acaaacctgt tttattaata tggcgacagg
      661 cggatttggg acgcgtatta ccacagaaac gccggaaaaa ttaaaagccg cgctgggtgg
      721 cgtctcttac atcattcatg gcttaatgcg catggatact ctgcaaccgg accgttgtga
      781 aatccgcggt gaaaactttc actggcaagg tgacgccctg gtcattggta ttggtaacgg
      841 gcgtcaggcc ggtggcggtc agcaattgtg tccgaacgcg ttaattaacg atggcttgct
      901 gcaactgcgc atttttaccg gcgatgaaat tcttccggct ctcgtatcaa ccttaaaatc
      961 tgacgaagat aacccgaata ttatcgaagg cgcttcgtcg tggtttgata tacaagcccc
     1021 acacgaaatc acttttaatc ttgatggcga accgttgagc ggacaaaact ttcatattga
     1081 aatacttccg gcggcgttgc gttgtcgatt accaccagat tgtccattat tgcgttaatc
     1141 aaactattct ctcttgaacc agggagaatc cgaaatgccg gatgcggtgt aaatacctta
     1201 tccggcatac agaacaatac tacaccgtaa ttaattttac ccgtgtcttc tgcaatgcca
     1261 gtttatccac ctctgataca gcatcatcgg taataataat gtcaattttt tcgattggca
     1321 gtacctgatt aaaaccgcga cggttaaatt tcgatgaatc gagaacggca atcactttat
     1381 tagcggcggt gaccatcgca ccgctaatcg aataaccttc attaaaggtc gtaataccat
     1441 taaccgcatc aataccatca gcaccgacaa acattaaatc ggcattaata tcctgcaggg
     1501 aacgttcagc aatagatcca tgcatagatc gcgttttatg gcggaccgtg ccaccgcaga
     1561 cgaccagagt aatgtcttta ttttcggaaa gggcaaacgc agccgggaga ctgttggtaa
     1621 tcaccgtgat atttttagcg gtcattaatc cttcggcgat aagcattgtg gtacttccgc
     1681 tgtcgagaat aacggtcatc ccttcatgga tcattttgac tgccgcctgc gctattctgt
     1741 ttttgggcgc gctggcgagc tgaaatcgct ctttaaaccc gacttcctgg gtctcggttt
     1801 cactattacc ggacattatt ttcgccgcac cgccatgaaa gcgcgtaacc acgccttttt
     1861 gttcgagaaa gcgcaaatcg gcacggattg tcgcttccga ggcagcaaat actcccgcca
     1921 gatcctgaac aagcacggtt ccctgttcat tcactaattg gatgatctta ttccttcgct
     1981 cgaatgaatt catcgtcatt tacccatatt caattgtggc tagtgtaaac gaagggggac
     2041 cattggtgaa tgatctgttt tgcacaatac cgggccagca tcacgctggc ccgcggtttc
     2101 atggaatgag caacactttg cccggcatgg cattacgagc gatgtcacgc accgcctggg
     2161 tgaagctttc aaagcttcca cggtgagcga ttaatggctc caggcttaac ttacgttctg
     2221 tcagcaaccg gctcgccgtt tcccactcct gccccggcca agggctggaa tagttcatcc
     2281 aactgccgat aaccgtcagc tctttacgca atattttgcc aaacgttgcc gatgttaaat
     2341 gcagatcctg atgcaacgtg cccaccagcg ccagttgggc atgaggaccg gcaatctcta
     2401 ccgccagttc gacagtttgc ggtacgccag ccgtctcgag gataagctga ttaaaacgca
     2461 gttcgcgtaa aacgctctgc atttgcggcg cgctcatttc gctactgtta aatgtttgca
     2521 tcgcaccgaa agattttgcc agtgccagtt tttctgagct aatgtcgata gccgtcacac
     2581 tctttgctcc cagcgcgaca gcgcactgaa tagccagcag gccaatggtt ccggcaccaa
     2641 taataataac gtttttattc tcacaacctt gcgctaaatg aaaagcatgc aagccaacgg
     2701 taatcggttc aataaaagcc ccatcctcaa taggcatatc agtaggtaga gcaaagacat
     2761 ttttccgctt aacgacaata tattcggcaa atccaccatc acgccgtgag ccaataaaat
     2821 catattttgc gcactgggaa taaaaccctt tcaaacactc tggacaagta aaacagggta
     2881 ataacggcac acaggcaacc gcatcgccag gatgtaaatc atcaacaccg gatcccaccg
     2941 catcaatata gccgctaaat tcatggccta acgttattgg ataataatgt gcaccatttt
     3001 taaatatcct gggtaaatcg gaaccacata agcccgagct ggcaattttt acccgcacct
     3061 catcctgatg tttaatttca ggaatgacgc tttctgcaac gcgcacgata ccatcagtat
     3121 cattcaccac tgatttcata aaaactcctg attgttaagg gggataacct ccccctcagg
     3181 taaaaattat tctgcgagaa cgactttctc ttgtttaata aagccacgcg ctctacgcca
     3241 ggtcatgaaa ataccggtaa gataaattgc gccgataata atgaatccgg gaatattttg
     3301 cggggagaaa acctgaatca gtaaccaggt aatgggagaa ccgccctgat ccattgaagc
     3361 caccataccc ccggctttta acgccccagc attagccgcc agttgggggt gcaaaccaat
     3421 agtttgcgtc gcgatccaca gagtgatgct catgatgatg acacccgaga ttaaggtgcg
     3481 gaacagattt ccacgatgca cggcgactgc catcgccaca aagaagccga tggtggcaag
     3541 atcgccaaac ggcagcacct gattacccgg aacacaaaca gcaattaaaa tggtgagtgg
     3601 gataaaaatc aggcttgccg ataccaccgc cgtatgcccc agcagtaatg cgggatccag
     3661 gccaatcagg aactcctgac cgccgaactt cgcctgtaaa cgactacggg cctgcttagc
     3721 gatgggcgtt aaaccatcca tgatgggttt aatcacccgt ggcatcagca gcataactgc
     3781 cgccgttttt accgccagct gcaatacacc tttgacatcg taacccgcga ggatgccgat
     3841 aatcagcccc atcacaaaac ccacggtgac aggttcgcca aaagggccaa atttgcgctg
     3901 aatatcgtcg gcgctaaatt taattcggtt aacgcctggg attttctcga tgatagcatc
     3961 gaccagcacc gcaatcggcc ccatatacgc cgacgtaccg tgcggaatag caataccttc
     4021 cagctcaaag aaatttcggg tatcgcgggc aaaccagtcg ccgagcttat aaacaaacgc
     4081 cgcgtgaatt accacacctg ccatccctat catccatgaa ccggttgcca ggtgcagcaa
     4141 cgcgccggtg aaggtcatat gccagatatt ccagatatca acatttacca cccgcgtcat
     4201 acgggtcagt aacatcgcca cgttaaccaa tatcgcaatc ggaatcgcca ccagcgcaat
     4261 ttgcgacgcc caggtcattg gtgaagagcc cggccagccg acatcgacca catgcagatt
     4321 caggtcgaaa ttttccgcca tcgctttcgc cgccggacca atggaatcca gcattaagcc
     4381 aatcacaagg ccaatgccaa caaagccaat cccgatatgc agacccgctt taaagcaatc
     4441 gcctgccttc atgcctaata ttttagaaaa aataatgatg acaaccggca gcatcactgt
     4501 agggccgagg tcgagaatat aacgcatgac ttctgaaaac ataggtcacc cctgtaagat
     4561 agtcagaatt ttattttgta atgcttcgat accgacacca gaaataaaag gcatgccgtg
     4621 aactaacgga atatcgccaa aactacgatc cactttggca gtggtgcata tcaaatgcac
     4681 accatccata taggtttcta tttcattaac ccgacactgg attaattcaa caggaatatt
     4741 atgattctga cacaactctt taatttcttc cgccgccatc gtagaggtcg caaccgcgcc
     4801 tccgcaagcg acaataatct tgcgtttcat aaatagtcct ttttattgtt acagagggat
     4861 ttattcgtct gaatctaaaa catactttgt gaagtattcc tttaactggg tttcaggaag
     4921 agtgattagt gtctcgacga tatcgggctg ttgtaacttg ccaaataaac agcgtaaaag
     4981 tttcaattgc tgctgcggat tttccacaat caacgcaata accaacgata cctccacgtc
     5041 gttatcatca tccgcttgct gaaaatgaac tttatttgtt ggccttaaca gataaatggc
     5101 tgacgactta gcatgaatcg cctcacaatg cggtattgca atggcgtgct gctcaagcat
     5161 tatcccggta gggaattctg cttctctggc aattaatgcc tgtggccagg tatcatgaac
     5221 cacacctttg gcgagcatct cattaccgat atgggttaaa acttcgctac gatcgacaaa
     5281 agaaattccg ctacgaacaa acaggttagt catataccgt ccttattccg cacagccgta
     5341 gcgataggcg cgcaaaacat cataaatttt atccataatc agctgatgcg gtattgctga
     5401 taattccccg gactgaatgc gttcaaattg tttgggaaga tactgactaa tcatgcccag
     5461 tggggtgtcc acgccttcaa gattcaccat catcgtttcg acgctatttt taatccgact
     5521 atgcggccag taataacgaa tacgatccga caggctgtaa cgaatatcca gtaatgaatc
     5581 gttaaaaccc gtacgataat attttttcca gtattgcggt tcgtcgagca tcacttcttc
     5641 aattaccgcc aggcaaccgc tgcgattttc gggggcgata agttcctgct caatttgtgc
     5701 cagtgcaaat atcgcctcgc gtaaagcaaa ggttaatgcg ggaccgactt tcaatattgc
     5761 aaagtgatcg cggactaatt cccaataagc cgtccgggtc tggtaatcgg tagaatgtgc
     5821 ttcataaacc attcgggtgt tttctatcca ttgcgccagc gcctgcgctt cctgcggctg
     5881 ataatggata atattgctgt gatcaaattc cacacccggc tgcaccacga tggcaatcac
     5941 acgtgttaac gcctctgtca gcccacgggc aataaaggcc ttttgatgcg tacgtaaagt
     6001 attggcggca tcttcaacat gggtgatgtg tactgactga atggcgctgg cctcaccgcc
     6061 cggaaccgga acttcggtgc caatgacata gctcagttgc tcacgctggc aatctgtcgc
     6121 cacactttcc gcagcaaagc aaagcacagc agctcgttcc gcaaccgttt ctggtgctaa
     6181 cggtatggga tcccccgcgc aggacattga cgcatcaaga tgaattttac tgaagccagc
     6241 acgaacatat gcttttacca gctcgacgga tttttccatc gccgcatccg cattttcttg
     6301 ctgccagcag tttggcccca gatgatcgcc gccgagaata atgcgttcgc gtgcaaaccc
     6361 aactttatcg gcaatcgtaa aaacaaattc gcgaaagtct gccggtgtca ttccggtata
     6421 accgccaaat tgattgacct ggtttgacgt tgcttcaatc agcactttgc gcgtgctgtt
     6481 gcgatcaaat gccagcgccg cttcgataac caacggatgg gcagaacaga ctgaacatat
     6541 gccgatatgt tcaccagctt tatgccgggc aattaacgtt ttcatgtttt ttccttgtta
     6601 aatggcaggt gcgttatgcc ctgccctcgc agccacaatc ggcaatcact ttgctcacca
     6661 catcgcgcat tgcggattta gccgactgca aataatcccg gggatcggtc gcttcagggt
     6721 gctcggtcag gtaatttttt aacgcctgcg agaaggcatt tttcagctcc gttgcaacgt
     6781 tgattttgca tatccccagt ttgatggttt gctgaatatc cttagtcgat aaccctgacg
     6841 cgccatgcag caccagcggt aagttcaccc actggcgaat gttctccagt cgagaaaaat
     6901 caagcgccgg tgcgctggca tacatcccat gagccgtgcc gatggcgacc gccagggaat
     6961 caattccggt tgcctcggca aattcacgcg cctgagcggg gttggtataa aaagcatcgg
     7021 cttcattgac ttgcacatca tcttcctggc cgccaagttg ccccagctcc gcttcgacgc
     7081 tgacatcaaa gcgatggcaa aaatccacca cctctttgac ccgtgaaata ttttgcgcaa
     7141 aaggcaaatg cgaggcgtca atcatgactg agcgcacgcc agaacgaacc ttctgagcga
     7201 tatcgtcaaa tttcgtgtga tggtcgagat gaattgccag tggatggtga tattgcttcg
     7261 ccatcgcgct gaccagcgcc aacagatttt ctgtaccagc atgagtaaat gtgccaggcg
     7321 ttccggcgat gatgaccggt gcatgcaggt tggcagcggt ttccaccacc acttgcatcg
     7381 tttcgagatt gtgaatattg aatgccggaa ccgcataacc gccgcgctgt gcgttgttca
     7441 gcatctgctt tgtcgatacc acgtacattt tcatatcctg tcgtttgttt tcgatatcaa
     7501 aatacaatga aatgatttgt ttttaaatgt cgagataacg atcacaaaaa cgacaatgtg
     7561 aaaattgttc gaggagtaaa aggcaaaaaa aacggcctcc cgatagggaa gccgtagcaa
     7621 agtgcgcgtg tttttatgcc ggatgcggtg taaacatacg tggcagtgct atgtaggcat
     7681 gataagacgc ttccgcgtcg caccaggcat aggttgccgg atgcagtgta aacgccttat
     7741 ccgtcctacg gggtggtgct gttcgtatga ggcgtatcag gcaatagtaa ttttgctatc
     7801 gagataaacg tcctgcacgg cgttaatcag tttcacgccg tcagccatcg atttcttgaa
     7861 cgccttacgt ccaagaatca gccccattcc gccagcgcgt ttgttgataa ccgcagtacg
     7921 cactgcatca ctgaggtcag tttcaccgcc tgcagcaccg ccggagttta tcaacccagc
     7981 ccggcccata tagcagttag ctaactgata acgcaccaga tcaatcgggt tttcgctggt
     8041 taacttgctg tacacgcgat cgtcggtata accgtaatta attgctttat agccgccgtt
     8101 attttccgcc attttttgtt tgacgatatc tgcaccgatg gttgccgcca gatggtttgc
     8161 ctgaccggtc aggtcggcgg aaacatggta atcaacgcca tctttcttaa aggcggagtt
     8221 acgcaaatag gcccacagca ctgtcaccat acccagctcg tgcgcacgtt caaaagccgc
     8281 agaaatttct tcaatctggc gacgtgactc ttccgagcca aaatagatag tcgcaccaac
     8341 cgcaaccgcg cccatgttga acgcctgctc cacgctggca tacagcgttt gatcgtaggt
     8401 attcgggtaa cttagcgtct cgttgtgatt aagtttgacg aggaatggaa tgcgatgcgc
     8461 ataacgccgc gataccgacg ccagcacgcc gtaagttgac gccacacagt tacagcccgc
     8521 ttcgatcgcc agttcaacaa tgtttttcgg gtcaaagtag agcgggttag cagcaaatga
     8581 agctccggca gagtgctcaa cgccctggtc aaccggcaga atagaaagat atcctgtgcc
     8641 agccagacgc ccggtgttgt acaacgtctg catattacgt aacaccgctg gcgggcgatt
     8701 attgtcaatc attacgcggt ctacgtagtc atgtccgggg agataaagct ggtcagaagg
     8761 aattgtcata caacggtgct gtaaaaggtt gtcggcgtct ttgccaagca actgcgcaat
     8821 atctgtcatt actatgctcc cgtaaattcc gattggatat cggctatgga ttgtcctggc
     8881 ccgccttttg cgggcaatca taatcctggt cgttacgggc gagtttttcc atcttttgga
     8941 tgttttttca gcgttttctg ttggctcgat tcatcagaaa aaatgttagc gcggtcaagt
     9001 tttcaccgca aaggtattta aaaggtatta ttaagtggta ttgtcatcgc gtaccttaca
     9061 ttacctgtca tgaaggaatt aaaagatgaa aacaacagca aagctgtcgt tcatgatgtt
     9121 tgttgaatgg tttatctggg gcgcgtggtt tgtgccattg tggttgtggt taagtaaaag
     9181 cggttttagt gccggagaaa ttggctggtc gtatgcctgt accgccattg cggcgatcct
     9241 gtcgccaatt ctggttggct ccatcactga ccgctttttc tcggcgcaaa aagtgctggc
     9301 ggtattgatg ttcgcaggcg cgctgctgat gtatttcgct gcgcaacaga ccacttttgc
     9361 cgggttcttt ccgttactgc tggcttactc tctaacctat atgccgacca ttgcgctgac
     9421 taacagcatc gcttttgcca acgtgccgga tgtggagcgt gatttcccgc gcattcgtgt
     9481 gatgggcact atcggctgga ttgcctccgg tctggcatgt ggtttcttgc cgcaaatact
     9541 ggggtatgcc gatatctcac cgactaacat cccgctgctg attaccgccg gaagttctgc
     9601 tctgctcggt gtgtttgcgt ttttcctgcc cgacacgcca ccaaaaagca ccggcaaaat
     9661 ggatattaaa gtcatgctcg gcctggatgc gctgatcctg ctgcgcgata aaaacttcct
     9721 cgtctttttc ttctgttcat tcctgtttgc gatgccactg gcgttctatt acatctttgc
     9781 caacggttat ctgaccgaag tcggcatgaa aaatgccacc ggctggatga cgctcggcca
     9841 gttctctgaa atcttcttta tgctggcatt gccgtttttc actaaacgct ttggtatcaa
     9901 aaaggtatta ttgcttggtc tggtcaccgc tgcgatccgc tatggcttct ttatttacgg
     9961 tagtgcggat gaatatttca cctacgcgtt actgttcctc ggtattttgc ttcacggcgt
    10021 aagttacgat ttttactacg ttaccgctta catctatgtc gataaaaaag cccccgtgca
    10081 tatgcgtacc gccgcgcagg ggctgatcac gctctgctgc cagggcttcg gcagtttgct
    10141 cggctatcgt cttggcggtg tgatgatgga aaagatgttc gcttatcagg aaccggtaaa
    10201 cggactgact ttcaactggt ccgggatgtg gacttttggc gcggtgatga ttgccattat
    10261 cgccgtgctg ttcatgattt ttttccgcga atccgacaac gaaattacgg ctatcaaggt
    10321 cgatgatcgc gatattgcgt tgacacaagg ggaagttaaa tgaaaacaga acgtattctc
    10381 ggtgctcttt atgggcaggc gttaggggat gcgatgggga tgccctccga gctttggcca
    10441 cgcagccgcg ttaaagcaca ctttggctgg attgaccgtt ttcttcctgg accaaaggag
    10501 aataacgcgg cctgttattt taaccgcgcc gaattcaccg acgatacctc gatggcgctg
    10561 tgtctggcgg atgcgttact ggaacgtgaa ggcaagatcg atccggatct gattgggcgt
    10621 aatattctcg actgggcgct gcgtttcgac gcctttaaca aaaacgtact aggtccgacc
    10681 tcgaagattg cgcttaacgc cattcgcgac ggtaaacccg ttgctgaact ggaaaacaac
    10741 ggcgtgacca acggcgcagc gatgcgcgtc tcgccattag gttgtttgct tccggcgcgt
    10801 gatgttgatt cctttattga tgatgtggcg ctggcgtcga gcccgaccca taaatccgat
    10861 ctggcagttg caggtgcggt agtcatcgca tgggcgattt ctcgtgccat tgacggagaa
    10921 agctggtcag cgattgttga ttccctgcct tcaattgcgc gacatgcaca gcaaaaacgc
    10981 atcactacct tcagcgcctc actggcagca cgtctggaga ttgcgctgaa aattgtgcgc
    11041 aatgccgacg gcaccgaatc cgccagcgaa cagctttacc aggtcgttgg cgcaggtacc
    11101 agcactattg agtccgttcc gtgcgccatt gcgctggttg aactggcaca aaccgacccg
    11161 aaccgttgcg ccgtcctctg cgctaacctt ggcggcgata cagacaccat cggtgctatg
    11221 gcgacggcaa tctgcggcgc gttgcatggc attaacgcta tcgatcctgc gttaaaggcg
    11281 gagctggatg cggtaaatca gcttgatttc aaccgctatg ccacagcgct ggcgaaatat
    11341 cgtcaacaac gggaggcggt atgagcggcg ttcgattaca cacgctgctg cctgaattaa
    11401 ccacgcgtca gccggtgatg gtcgtcggcg cggcggtcat tgacgtgatc gccgacgctt
    11461 atgccctccc ctggcgtggg tgcgatatcg aactgaaaca gcagagcgtt aacgttggcg
    11521 gctgcgcact gaatattgcc gtggcgttaa agcgcctcgg catcgaagcg ggtaatgcct
    11581 tgccgctcgg tcagggcgtg tgggcggaga ttattcgcaa ccggatggcg aaagaggggt
    11641 taatcagcct gatcgataac gccgaaggcg ataacggctg gtgtctggcg ctggttgagc
    11701 cggatggcga acgtactttt atgtcattca gcggtgtgga aaatcagtgg aatcgccagt
    11761 ggctggcgcg attaaccgtt gcgcctggca gcctgctcta tttttccggt tatcaactgg
    11821 cctcgccctg cggcgaattg ttagtggaat ggctggaaga gctgcaagac gtgacgccgt
    11881 ttatcgattt tggcccacgt attggcgata tcccggacgc attactggcg cggatcatgg
    11941 cctgtcgacc tttagtgtca ctcaatcgtc aagaggctga gattgccgcc gaacgttttg
    12001 ctttatccgc agagataaca acacttggca agcaatggca ggagaaattt gccgcgccgt
    12061 tgatcgttcg tctcgataaa gaaggcgcat ggtatttcag caacgacgct tctggctgca
    12121 ttccggcata tccaacgcaa gttgtagata ccattggggc gggcgacagt catgcaggtg
    12181 gcgtacttgc cgggctggct tctggtctgc cactggcgga tgccgtatta ctgggcaatg
    12241 cagtggcgtc gtgggttgtc gggcatcggg gcggtgattg tgcgccaacg cgagaggaac
    12301 tactcctcgc acacaaaaac gtatagatcg ctgcgacagt ggctaatgct gtactcaata
    12361 ggccgttgct gttggtcaag cgccacttgc ttgatcacca gcaccggtat tttgctatct
    12421 aactgaatat gtgactgaaa ctcggcatcc ggcatccggg cgctaacgcg ggaacgcgta
    12481 cgttgtgggt aaatgtgctg gctgcggaaa tagtcataaa gagaaattcc gatggcatca
    12541 acatcatgaa ttaaatgcgc cggaacccac gattcctcaa tcgataccgc ttcttcatcg
    12601 acatagcgaa tacgcttaag caaaaagaca tcacttcccg cttcgaccgc cagctgctgc
    12661 gcgacttctt cgggacattt cacgacacgc ttgttaaccc atagcgtatc gggctttttc
    12721 ccgcgcaata ccacctgctg agaaaaacct cgcgcttctt tcaacgagta ttcgaagatg
    12781 ttgttgatct gcgtgccgta accgcgcgaa cgcgtcacca caccctcttc ttccagcgcc
    12841 tgcatcgcct tgcgcaccgt aatgcgtgaa acgccggtta actgacttaa atcacgctca
    12901 ccgggcaaaa tattgccatg ctccagcacc ccgctgcgca cggcattttt taccgtttcg
    12961 gcaaacttaa tataaagcgg cgtgttatcc gccgctaaaa tacgctcgtt cagttgggca
    13021 atcagctggg tatgcgcttg ttccatttgt ctttttcctg ccagggagtc tggtgggagt
    13081 atatcactgc ttcttattta aggcgtttct ttaatcccat caacgaatgc ctgcaactcc
    13141 tccaccgtgc cattaaatac attaaaatcc accggaccat tgatgccatc tacctgtcca
    13201 cggtcggaat gctgccagaa gcgccaggcc atgccgtcat tgtccggacg acgttgatag
    13261 tagtgagcca cccaccacgg atattcattg aaatagcccg ccagattggt gtgataaaaa
    13321 acggctcctg agtaaataat cggctttttt cccgtacttt tttcgaccat ttttagccac
    13381 tgacttaccc gcttgcgtaa ttcttttgcc gataattttc cccgttcttc tacgtccagc
    13441 acggcaggga aatcgccttg tgagaaatcc accgtttgca gaaataatcg cgcctgaaca
    13501 ggcgctgcta ccgacgggga gaaataatga tacgccccgc gcagcaggcc attttcgcgg
    13561 cttagttgcc agttacgcga aaaatagggg tccaccagct tttcgccttc cgtcgccttg
    13621 ataaaggcaa attgtaagcg gataccgtta tcgcgcattt ttgccacccg ctgccagtcg
    13681 atccgctcct gccagcgcga aacatctatt ccgtgaatgg tgtaactggc aggaatacga
    13741 atagcgaaag atttaaccgg gcgataaccg tagaagtgga tgtagtctgt aatctgaact
    13801 gctgtcgaat agaaaaaatt caccgtctga cgcggataaa ttgcaacgat ggaaattagc
    13861 ccaagagcgc ataatagagc ggtgagtttt tttcgactgg taattcttag ctgcattggt
    13921 ttcatccctg aatgtcagtg ccaaaggctg acaataacca aagcagctat agtacggtgc
    13981 tcaccaccag gcgtggaagt ggtgaaccgg accaataccg tgaccaactt ccagcgtgtc
    14041 ggcctgggct aacgccgatg aaagccagct ttttgcctcc tgtacggtgt cagcccagtt
    14101 tgtatggcgc gggcgtagtg cagccaacgc cgcagagagt gtacaaccag tgccgtgggt
    14161 gtttttggtc ataatgcgcg gtgcggtaaa ccgttgttca ccctcgcggg taaacagcca
    14221 gtccgggctt tgctcatcat ccagatgacc acctttcatt agcactgcgc cacagcccat
    14281 cgccaacagc gatcgccctt gttccagcat ttcctgttcg gtgcgcgcgt gtggcgcgtc
    14341 gagcaaggcg gcagcttcgg gcaagtttgg cgttattaat gaaacctgtg gcaataatcg
    14401 actgcgcagc gtagcaaccg ccgaaggtga aagcagcggg tcgccgcttt ttgccagcat
    14461 aacggtgtcg agtaccacgt tttggatctg ataacgttgc aaccgttctg ccaccgcttc
    14521 aacaatatcg gtttccgcca acataccgat tttagtggta tcgattcgca catcgctgaa
    14581 caccgaatcg agctgggcgg cgacaaaatc aggctcaatg cgatacaccg actgtacgcc
    14641 acgggtattt tgcgccacca gtgcagtaat aactgagcaa ccataagcgc caagtgccga
    14701 gaaggtttta agatcggcct gaatccccgc accaccactc ggatcagtac cggcaatcgt
    14761 cagagcgtta attcgtttca tgcctgcacc tcctgcgtca attgccagag cgcatcaagg
    14821 aaatgtggaa caaaactgcc tggcccctcg cttctggcga ctgcgcgttc tccggcttgt
    14881 ttcatccagt gacaggcaga tgcgacattt tccagcatat cgcctggtaa cgcacagcag
    14941 gcagcgacaa ctgccgataa tgcacagcca gttcctacca ctttggccat taacggatcg
    15001 ccgccgtgaa tgccaacggt gcgacgacca tcggtaacat aatccacctc gccagtgacc
    15061 acgacgattg cgccagtttc ccgtgccagt gtttgtgcag cgggtatcgc attagctgcc
    15121 gcgtcagtgg tatccactcc ccgtccgcca gtagcaacgc cagctaatgc catgatttcc
    15181 gaagcattac cacgtatcgc tgccggttta aaagataaaa gttcatgaca aaaatggcga
    15241 cgataatcga gcgcaccaac cgctactgga tcaagcgtcc agggggtttg agagcttttt
    15301 gcttgctcaa cggcggcacg catcgcctgt gcgcgtggct gcgtcagcgt gccaacgtta
    15361 atcaacaagg cactggcgat agccgcaaac tgactggcct cttcggtttc gataaccatc
    15421 gctggcgatg caccgatcgc cagcaaggta ttggcagtaa aggtttgcac cacatcgttg
    15481 gtcatgcaat gcacaagagg ggaatgttgg tgaaaaaggt gtaacgtgtg cgcagattgc
    15541 gcggaactca gcaggtcgac ttgcatagtt tgctcctgcc ataacgtgaa gaagcaatga
    15601 cctggtggtc cgtgacttcc ctacgctggc attatccaga tcaggtgata cgggtatttc
    15661 tcagccttca caaagaaggg caccccgagt cgtttggttg cgatgataaa tcgcagagga
    15721 ggatggtaat gtccagcgca cccgttgtaa acgaggaaaa acggcagttt tacaatcgcg
    15781 ttaatatatt atttgatata tgaatccagc accttcagaa cgacatccag atcttcttca
    15841 cgttttagct catccccctg gtgaacgata tgttccgtta gatgaccttt aatcacttcc
    15901 cgcatcagac cgtttaccgc gccacggata gcagcaatct gctgtaaaac tgcagcgcat
    15961 tcgtgcggct cgtcgagcat tttcttgagc gccacgacct ggccctgaat cttactggca
    16021 cgtgctttca gtttctgttt atcacggatt gtatgagaca tggcaacacc tggttaacaa
    16081 gaacatgaaa aatcatagca ctattaatct actggggggt agtatcaggt actggggggg
    16141 agtagaatca gattgccgaa ttaatactaa gaattattat catgaccgaa tttacaactc
    16201 ttcttcagca aggaaacgcc tggttcttca tccccagcgc catcttactt ggtgcgcttc
    16261 atggcctgga accagggcac tcaaaaacga tgatggcggc gtttatcatc gccatcaaag
    16321 gcaccactaa acaggcggtg atgctcggac tggcagcaac catttcgcat accgcggtgg
    16381 tctggttgat tgcctttggc gggatggtga tcagcaagcg ctttactgct caatcagcag
    16441 aaccgtggct ccagttgatt tccgcagtga tcattattgg caccgcgttc tggatgttct
    16501 ggcgtacctg gcgcggcgaa cgcaactggc tggagaatat gcacgagcat gatcatgaac
    16561 atcatcacca cgatcacgaa catcaccacg accatggaca tcatcaccat cacgaacatg
    16621 gcgagtatca ggatgcccat gcacgagccc atgccaatga cattaaacga cgctttgatg
    16681 gtagagaggt caccaactgg caaattttgt tatttggctt aaccggtggc cttatcccct
    16741 gcccggcagc aattaccgtg ctgttgatat gcattcagtt gaaagccctg acactgggcg
    16801 caacactggt cgtcagtttc agcattggcc tggcgttaac gcttgtcacc gtaggcgttg
    16861 gcgcagcaat cagcgttcag caggtcgcaa aacgctggag cggatttaac actctcgcta
    16921 aacgcgcccc ctatttttcc agtctgttga ttggcttagt tggtgtgtat atgggcgtac
    16981 atggcttcat gggcataatg cgataactca atcctttagg cttgcatgtt atcttcagcc
    17041 caggatgatt cttaaatcag ctatttccgc tgactctctg ctcgataagg atgcttttag
    17101 ggcatccttt catttacact ttttacgaaa tcatgggatc actaacaaaa tatcgcttgt
    17161 cagttatatt gtatggcagg aaagatatgc gactgatatt acagatcccc aaagtggaga
    17221 gtttatgacc attaaaaata agatgttgct gggtgtgctt ttgctggtta ccagtgccgc
    17281 ctgggccgca ccagccaccg cgggttcgac caatacctcg ggaatttcta agtatgagtt
    17341 aagtagtttc attgctgact ttaagcattt caaaccaggt gataccgtcc cagagatgta
    17401 ccgaaccgat gagtacaaca ttaagcagtg gcagttacgt aacctgcccg cgccggatgc
    17461 cgggacgcac tggacctata tgggtggcgc gtacgtgttg atcagcgaca ccgacggtaa
    17521 aatcattaaa gcctacgacg gtgagatttt ttatcatcgc taaaaaaagc cccctcatca
    17581 tgagggggaa atgcagacac cttgttattt tttattatta gccacttgct cgtcttgctt
    17641 gttattagtc gtatttcacg ttgattagtg cggttgcctc cagtgcgcca gatttaactt
    17701 tgtttgtatc gtagacgtag taactggctg ttatcggaat tgaaaccgtt gtatctgtaa
    17761 gttttgactt tgccgcaatt tcttttaaat ttgtgtatcc tttactgctg ttagtgatat
    17821 ttaaaagtgt gactggcgaa ccattacttt caagaataaa tcctacgcca ttatcttccg
    17881 cacctttcag tattgtgttg ctgttggtta acaagtcaga acttgataaa tacacctgca
    17941 ttttccgtga tgtttgattt tgttgaccta ataaattttt acaacgcaga ataatatccc
    18001 cttttttact atttactgtt gctttgttac ctgtggctgg taattgtgaa acgggaatac
    18061 tctctaaagt aattaataaa tcgtccggtt tgcaggttgt ggttatcggg ttgtatgaca
    18121 atgtcagatt aaaggaatag acacctgcat tttgtgccaa tgcatttctg tagctattta
    18181 cacatgcatc ccataacttg ccacctaata aacacagtag aaattgtact gcggcactcc
    18241 cgcgtgtcga tgatgaagca ctggataatg tcgctacacc attaatcatc actgaaccgc
    18301 cgccccccgc agaaacattc acttttgcat tactggccgc aggtgtcacc gttaccgaat
    18361 aaccactggt gaaactggtc tgattatttg acaacg
//
LOCUS       DAPGOF010000093        18238 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.093, whole genome shotgun sequence.
ACCESSION   DAPGOF010000093 DAPGOF010000000
VERSION     DAPGOF010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18238
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.093"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1..545
                     /locus_tag="RHV13_002823"
                     /pseudo
     CDS             1..545
                     /locus_tag="RHV13_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            648..1151
                     /locus_tag="RHV13_002824"
     CDS             648..1151
                     /locus_tag="RHV13_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HDV1242343.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKKFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1218..2675)
                     /gene="nuoN"
                     /locus_tag="RHV13_002825"
     CDS             complement(1218..2675)
                     /gene="nuoN"
                     /locus_tag="RHV13_002825"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HDV1242344.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2682..4211)
                     /gene="nuoM"
                     /locus_tag="RHV13_002826"
     CDS             complement(2682..4211)
                     /gene="nuoM"
                     /locus_tag="RHV13_002826"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HDV1242345.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4375..6216)
                     /gene="nuoL"
                     /locus_tag="RHV13_002827"
     CDS             complement(4375..6216)
                     /gene="nuoL"
                     /locus_tag="RHV13_002827"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HDV1242346.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6213..6515)
                     /gene="nuoK"
                     /locus_tag="RHV13_002828"
     CDS             complement(6213..6515)
                     /gene="nuoK"
                     /locus_tag="RHV13_002828"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HDV1242347.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6512..7066)
                     /gene="nuoJ"
                     /locus_tag="RHV13_002829"
     CDS             complement(6512..7066)
                     /gene="nuoJ"
                     /locus_tag="RHV13_002829"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HDV1242348.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7078..7620)
                     /gene="nuoI"
                     /locus_tag="RHV13_002830"
     CDS             complement(7078..7620)
                     /gene="nuoI"
                     /locus_tag="RHV13_002830"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HDV1242349.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7635..8612)
                     /gene="nuoH"
                     /locus_tag="RHV13_002831"
     CDS             complement(7635..8612)
                     /gene="nuoH"
                     /locus_tag="RHV13_002831"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HDV1242350.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8609..11335)
                     /gene="nuoG"
                     /locus_tag="RHV13_002832"
     CDS             complement(8609..11335)
                     /gene="nuoG"
                     /locus_tag="RHV13_002832"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HDV1242351.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSLE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQCRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11388..12725)
                     /gene="nuoF"
                     /locus_tag="RHV13_002833"
     CDS             complement(11388..12725)
                     /gene="nuoF"
                     /locus_tag="RHV13_002833"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HDV1242352.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12722..13222)
                     /gene="nuoE"
                     /locus_tag="RHV13_002834"
     CDS             complement(12722..13222)
                     /gene="nuoE"
                     /locus_tag="RHV13_002834"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HDV1242353.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13225..15027)
                     /gene="nuoC"
                     /locus_tag="RHV13_002835"
     CDS             complement(13225..15027)
                     /gene="nuoC"
                     /locus_tag="RHV13_002835"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HDV1242354.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15121..15783)
                     /gene="nuoB"
                     /locus_tag="RHV13_002836"
     CDS             complement(15121..15783)
                     /gene="nuoB"
                     /locus_tag="RHV13_002836"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HDV1242355.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15799..16242)
                     /gene="nuoA"
                     /locus_tag="RHV13_002837"
     CDS             complement(15799..16242)
                     /gene="nuoA"
                     /locus_tag="RHV13_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HDV1242356.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16873..17811)
                     /gene="lrhA"
                     /locus_tag="RHV13_002838"
     CDS             complement(16873..17811)
                     /gene="lrhA"
                     /locus_tag="RHV13_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HDV1242357.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPISAPEGDDSLLIERDIE"
BASE COUNT         4557 a         5059 c         4715 g         3907 t
ORIGIN      
        1 cgacacgtaa ttcctgagca attagaaaaa atagttcgtt atcttacaca aactgtgcat
       61 ccacgtaatg ccgacaataa tgataatctg aataggtccg tagagtatat aaaagaggtc
      121 tttgtcagta gcggtgccag agttaccgcg caggacgtcc ccgattggct attacgactc
      181 tgcgcctggt agccaggatt acccttaccc gcaatgtcct ggctttatcc cgatcggggg
      241 gactttattg cctggtcggc agaatacagg atatcaacgc cgttcgtcag gtaaaaacgg
      301 cattgttatc atcccgggat ttatctgttt attctatgaa tgccccatgg tttattaacg
      361 gtattaattt ctctgaccgc cttaattatt ggcaacacga tattcccgcc gtaatgatta
      421 ctgacaccgc tttttatcgt aataaacaaa actacttgcc cggtgatatc gcagacagat
      481 tgaattatca gaaaatggct caggtggcgg atggtgttat aaccttgtta cacaacagta
      541 aataataaaa atttctcgag gcgggtccag aaacagcgac atgtacattt tattttccct
      601 cgtctgtttt aagatgcgaa acataaaatt caatggagga aaaataaatg ggaatgattg
      661 gttattttgc agaaattgac tcagaaaaga ttaaccaact gcttgagtct actgaaaaac
      721 ctttgatgga taatatacat gacacgcttt caggattacg ccgactggat attgataaac
      781 gctgggattt tttacacttt gggctgacag gcacctctgc gttcgatcca gcgaagaatg
      841 atccgctgag tcgtgccgtg cttggtgaac acagcctcga agatggtata gatggtttcc
      901 tgggattgac atggaatcag gagttagccg cgacaatcga ccgactggaa tcactggatc
      961 gaagtgagct gcgtaaaaag ttttccatta agcgactcaa tgaaatggag atttatccgg
     1021 gagtgacctt ttcagaagag ttagaggggc aactctttgc ttcgatcatg cttgatatgg
     1081 aaaagctgat tagcgcgtat cgacgtatgc tgcgacaagg gaatcatgcg ctgacggtta
     1141 ttgttgggtg atggtgtcag ggcaccaaaa aaaaccgccg aacatgtcgg cggctttctg
     1201 acttacaaac tgatagctta catcagcggc attgctaacc gcacaatgct aatcagcggt
     1261 tgtggccata cacccagtac cagtaccaac agtgcagaga tcagcacaac gataccgccc
     1321 gcgctgtact gccagtttga tggtgcatcg cgacccggtt gttccggtgc gtgcaggtac
     1381 aggctcaccg ccacgcgcag gtagtagtag aggccgattg ccgaaccgac aaccacggca
     1441 cccaccagcc accacaagtg tgcctggaca ccgaccgcca gcacgtagaa cttaccgata
     1501 aagcccagcg tcatcgggat accggccaga gacagcatca tcaccgtcat cactgccgcg
     1561 aggatcggac gatgccagaa cagaccgcgg taagagaaca gggaatcagc atccgggcca
     1621 cgatacgggc tggacatcag gctgaccacg ccgaacgcgc cgaggctgct gaacagataa
     1681 ccggccaggt aaacccctac cgcttccatc gacatctcgc ccgtttgcag cgcaatcagc
     1741 gccaccagca gatagccgag gtgagagata gatgagtaac cgagcagacg tttgatattg
     1801 gtctggctca gcgccatcag gttaccgaag atgatggagg caaaggcgat aatcgccagc
     1861 accacacgaa tcgcttcgct gtcacccacc ggtgcgtaga ggaacagacg catcactaca
     1921 ccgaagatag cgattttgct cgccgtcgcc aggaaagtgg aaaccggcgc aggcgcgccc
     1981 tggtatacgt ctggcgtcca caggtggaac ggcaccagag agagtttgaa gccgaggcca
     2041 acaatcatca gaccgaaacc tgccagcaac agcggctcgt tgagcatacc gtcgccgagg
     2101 tttttaccca gcgcgacaaa cgacaggtcg ccagactgcg catacaccag cgccataccg
     2161 aacagcagga aagaagacgc tgcggcagaa aggatggtgt atttgatact ggcttccagc
     2221 gaacgtttct ggcggaaagc gtaaccgacc aggccaaaca gcggcaaaga gatcagttca
     2281 ataccgagga acagagacgc cagatggttg gcattcgcca gcaggatccc gcccagggcg
     2341 gcaattaaca ccaacaggta gaactcatcc ttgttgtcgt tatagccttc aagccacggg
     2401 taggcgaaag tacaggtggc gaggctcgcc aacaatacca gcccggtgta aagcatggcg
     2461 aaaccatcaa cgcgcatcag cggcgtaacg tccatagcgc ccgcctggcc aacaaaccag
     2521 agcgaaacca gcgccgcgtt aagcccaata accgagagcg tagcgttgag gaaatgattg
     2581 cgtcgccacg caatggagag catcacaacc accaccgtca agccgacgat cagcaacggt
     2641 agcagtgcga tcaggttttg tggagttatt gtcatggcga tttacggcct tgtagtagta
     2701 acggaattaa caaaccactg ctggatattg ccaatcgcgg agtgcgaggt atccagaatc
     2761 ggctgcggat agaagcccag cagtaccagc agcaccacca gcaacaggat cataaacagc
     2821 tcacgcagcg acatccctgg cagttcctgg ctggcaatct ggcttttcgc tttaccgaag
     2881 taagcgcgat gcaacatcgc cagcgaataa acagatgcaa agaccagccc aaaggtagaa
     2941 ataacggtaa tcaccgggac aacctggaag ctgccgaaca gaatcataaa ttcgccgacg
     3001 aagttaccgg tgccaggcat cccaagcgtt gccaccgcaa agaacagcga cagtgctggc
     3061 agccatttca tcttgctcca cagaccgccc atcatgcgca tgtcgcgggt atggatacgt
     3121 tcataaagct gaccgcagag aataaacaga cccgccgccg acaagccgtg cgcaatcatc
     3181 tggattaccg cgccctggta ggccaactgg ctgccggtgt agatagcaat cagcacgaag
     3241 cccatgtggg aaaccgaggt gtaggcgatc agacgtttga tatcggtctg ggcgaaggcc
     3301 atccacgcac cgtagaagat gccgataaca cccagccaca tagcgattgg cgcgaactct
     3361 gccgacgcat ttgggaacag cggcagggag aaacgcagca aaccgtaagc ggcagttttc
     3421 agcaagatcc ccgcgaggtc aacggaaccg gcggtcggag cctgggagtg cgcatccggc
     3481 agccagccat gcagcggaac caccggcatt ttgactgcga aggcgatgaa gaagcccagc
     3541 atcaacagat attccacacc actggacatt ggcgtattca acagctcttc atagttgaag
     3601 gtccagacgc cggtcgcatt gtagtgaaca aaaaccagcg ccaggatggc gatcaacatc
     3661 accagaccac tcgcctgtgt gtaaatgaag aacttggttg ccgccgtgat acgcgtttta
     3721 ccatcagagg ctttatgccc ccacagtgcg atcaggaagt acatcggcac cagcatcatt
     3781 tcccagaaga agaagaacag gaacatgtcg atggcaagga acacgccgat aacgccgccc
     3841 aggatccaca tcaggttgag gtggaagaag ccctgatatt tttcgatctc tttccacgaa
     3901 cagagtaccg ccagcacacc gagcagaccg gtcagcacga ccattagcag cgacagcccg
     3961 tcaatggcga gatggataga gataccaaaa cgcgggatcc acggcatgtc gaattcagac
     4021 tgccactgcg gaattccggc ggattgcgtc agtgaataac cgccctgcaa ccacagttgc
     4081 agcgacagcg ccagcgtcaa tcccatggtg atcagcgcga tccagcgcgg caccttgacg
     4141 ccaaagcgtt cggtctgcca gcacaggaag ccgccgataa agggaattaa tattagccag
     4201 ggtagtaaca tggcgatctt tattccttgt aaaagtcccg tcaggaccgg attttcaacg
     4261 aattctcacg acagaaaatt ctcttcggac tgggggtagt gtcggatgca ccgcgaagga
     4321 ggcttatccg acctacgttc ttatatgccc aggggcaatc ccacaatcct taactcaacg
     4381 cagtaccatc aacagtgcca gcaccacgac cgcaccgatg ctcatggatg ccacatacca
     4441 gcgcaggtag ccgttctcgc ttaacagcag acctttacct gcaaagcggg aaagaaccgc
     4501 cggaatgttc atcattgagt tcagcggatc gcgtttcagc aaccaggcaa tacccaggaa
     4561 cggcttgacg aacactttgt cgtacagcca gtcaaagccc caggcgttgt accaccaggt
     4621 gcccagcaga cggcccggcg cactgttggc gatggaggtc accagagtac gtttacccag
     4681 ccacagccag gcagccagca gaatgccgac caccgcgacc acgccagagg taatttccag
     4741 ggtcaacatg ctgccgtgcg ccagctccgt agtttgcgga agcacgccct gcagcggcgg
     4801 tacaatcagt gcgccaacga aggtggaaag gatcagcagc acaatcagcg gcaggctgtg
     4861 agttacccct ttcacggcgt gagcgtgaat ttgttctttt ccgtggaaga cgatgaaaat
     4921 catacggaag gtgtagagcg aggtcataaa cgcaccgacc agacctgcca ccatcagatt
     4981 gatatgacca ttcgccatcg cacccgcgag gatctcatcc ttactgaaga agcccgcagt
     5041 gaccagcggc agtgccgaca gtgctgcgcc gcccaccagg aagcagagat aaaccagcgg
     5101 aatagattta cgcagaccgc ccatcttgaa gatgttctgt tcgtgatggc aggccagaat
     5161 gacggaaccg gatgccagga acagcagcgc tttaaagaac gcgtgagtca tcaagtggaa
     5221 aatcgccgca tcccatgcct gcacgccaag cgcgaggaac atgtagccaa tctggctcat
     5281 ggtagagtaa gcgagaacac gtttgatgtc ggtctgtacc agcgcggcaa aaccggccag
     5341 cagcagcgta accgccccaa caatgcccac cagatgcaga acttccggcg tcatcaggaa
     5401 caggccgtgg gtacgggcga tcaggtagac acccgcggtt accatcgttg cggcgtggat
     5461 cagcgcggag acaggcgtcg ggcccgccat cgcgtcggca agccatgtct gcaacggcaa
     5521 ctgcgcagat ttaccgaccg caccgccgag cagcatcagc gtcgcccaca tcagcatgtt
     5581 attgccgtca gcaaagtgcg ctggtgccag ttccaccatt tcgcggaagt tcagggtgcc
     5641 cagttcgttg taaagaatga acagtgcgaa agcgaggaaa acgtcaccca cacgggtcac
     5701 gacgaacgct ttcattgccg ctgcgccatt cttcggatcg gtgtaataga aaccgatcag
     5761 cagataggag cacaggccca cgccttccca gccgaggtac atcagcagca ggttgtcggc
     5821 aagcaccaga actaccatgc tggcgatgaa caggttggtg taggcgaaga agcgagagta
     5881 gccctcttca ccgcgcatgt accaggaggc gtacatgtgg attaggaaac ccacaccagt
     5941 gaccaccgag agcatggtca gcgacagacc gtccagcacc aggttaaaac cgatgttaaa
     6001 gtcgcctacc gacatccacg tccacagcgg ctggctgtat gcctgctcgc cgttagcgaa
     6061 aaagtcgacg ccgataaagg cggttaccag cgccgccagg cccacagagc ctacgccgac
     6121 gatcgccgag acgttttcag accagcgccc acgggagaac gccagcagga cgaagccaat
     6181 caatggcaaa ataatggtta aggcaagcat gttcatccgc gcatctcact tactgaatcg
     6241 atgttcaggt tctggcgacg acggtgaagt tgcagcagca gcgcaaggcc gatactcgct
     6301 tctgccgccg cgaggctgat ggcgagaatg tacatcacct gaccgtcggt ctggccccag
     6361 tagcttccgg cgaccacaaa ggccagcgcg gaggcgttaa tcatgatttc cagaccaatc
     6421 agcataaaca gcagattgcg acggataacc agaccggtta agccaagaac gaataagatt
     6481 gccgcgagga tcagtccatg ttgtaagggg atcatgcgtg ctcctccgtt tttcttttcg
     6541 cgctgtcgtc tttacgattg ctcagcactt cacccgcacg ctcttcacga ccgacgtgga
     6601 aggccacaac cagacccgcg agcagcagca tagaagccag ttccaccgcc agtacgtaag
     6661 gcccgaacag cgtaataccc actgctttag cactgattgg cgtaccgtcg ataccttgat
     6721 cgttaacacc gagaatggcg taaacaatca ccaccaacat gatggctgac aaaattgccg
     6781 gaccaatcca cacctgcggt ttcagccact ggcgttcttg ttcgatttct gaaccgccca
     6841 ggttgagcat catcaccacg aacacgaaca gcaccataat ggcacccgcg tagacgataa
     6901 tttccagcgc accggcgaag taagcgccca gtgagaagaa caccccggaa atcgccagca
     6961 gcgaaataat caggtacagc agtgcgtgta ccggattggt atgggtgatc actcgcaagg
     7021 tcgcaagtat ggctatcagg ccacagatat aaaaagcgaa ctccattgcc cctctcctta
     7081 cggtaacagg ctcttgacgt cgataggctt ggcttcgttc tctgcttcgc ccttatcttt
     7141 gccgtcgatt gccatacctg ccatccggta gaagttatac tccgggtatt tgcccggacc
     7201 ggagatcagc agatcctctt tctcgtaaac cagatcctgg cgcttgtatt cccccatttc
     7261 gaaatccggg gttaactgaa tcgccgtggt cggacaggct tcttcgcaca gaccacagaa
     7321 aatgcagcgt gagaagttga tgcggaaaaa ttccggatac cagcgaccgt ctttggtttc
     7381 tgctttttgc agcgagatac agccgaccgg gcaggctacc gcgcagaggt tacaggctac
     7441 gcaacgctct tcgccgtccg ggtcgcgggt cagaacgata cgaccacgat aacggggcgg
     7501 cagatagacc ggctcttccg ggtacattcg cgtttcgcgt ttggcgaacg cgtgcaggcc
     7561 gatcatccag atactacgaa cctgggtgcc gaaacctact aacaattctt ttaaggtcat
     7621 ggtcttattg ccccttattg cgcctgccag agaatgacag ccgccgttac cagcaagttg
     7681 atcagcgtca gcggcaggca gattttccag ccgaaggaca ttacctggtc ataacgcgga
     7741 cgcggtaacg acgcacgaat caaaatgaac atcatcataa agaacgcggt tttcagcgcg
     7801 aaccagatga atggcggtaa caacgggcct tgccagccac cgaagaacag cgtcaccatc
     7861 aatgcagaga tggtcacaat cccgatgtac tcacccacga agaacagacc gaacttcata
     7921 ccggaatatt caatgtggta accatccgcc agttcctgct cggcttccgg ctggtcaaac
     7981 gggtgacggt gacacaccgc cacgcccgcg atggcaaagg taataaaacc aaagaattgc
     8041 gggatgacgt tccacacatg cgcctggctg ttgacgatgt cggtcatgtt gaatgaaccg
     8101 gcctgcgcca ccacgcccat cagggaaagc ccgaggaaca cttcgtagct cagggtctgc
     8161 gcagaagcac gcatcgcacc gagcagcgag tatttgttgt tacttgacca gcccgcaaac
     8221 agcaccgcgt aaaccgccag accggccatc atcaggaaga acaaaatccc gatgttcagg
     8281 tcggcaacca cccaacccgg actgactggc acaatcgcaa aggccagcag cagcgaggta
     8341 aacgcaatca tcggtgccag ggtaaagatg acgcgatccg agaatttcgg gatccagtct
     8401 tctttaaaga acattttgat catgtccgca accagctgga gcgaaccacc ccagccaaca
     8461 cggttaggtc cgtaacggtt ctggaacaga cccagcaggc gacgttcgcc aaagctcatg
     8521 aatgccccgc aggtgacaac caccagcagg atcaccaccg ctttgaggat ggtcagcagg
     8581 atctcaatca gttccggtga tatccaactc attgttgtgc ctccttgaga tcctcaagat
     8641 gcgcgccagc cagcaccgga gcaatgccgg acatacccat cggcaagccc acctgccctg
     8701 ccgtcagccc ttcagcgatt tcaaccggca gagtgaccgt gttgccatcg taactaaagg
     8761 agacgcgtgt acctgcgttt acacccaact tcgcggcatc cgctgggttg agtttgatgt
     8821 acggctgcgg catacgacac tggaagaccg gagcacgctg tgacaattca tcgctgccaa
     8881 acaggtggta gtacggcgcg atacgccatt tcccgtcctg cggctggaag cgtgccggta
     8941 cgctggtgaa gtaatccaga ccattttcgc tggtttcaaa cagacgcaca cccggatcgc
     9001 caaagcgcag tttgccgccc acttcgtcct ggaatttgtt ccacgcctgc ggggagttcc
     9061 agcccggagc ccaggcaaac ggcacttgcg aacggtgcgc ggtcggctgg ttgttacctt
     9121 ccatcgagaa ggtgaacatg gtgtcaatat cctgcggctg acgcggctca tgaacgctga
     9181 tattggcgcg catggcggta cgaccgctgt aacggtgcgg ttcacgggcc agtttctgcc
     9241 cacgaatacg gaatgtcgca tctggcgcag catctttgat acccgccagt tccgggattt
     9301 tcgccacaac agcgtcaatc acatggtcga gctgcgtcca gtccacttca cggctcagca
     9361 aggtgctgtg cagcgagtgc agccagcgcc agctttccag catgacagtt ttgctgtcgt
     9421 aataagccgg atcgtaaacc tggaagaaac gttgggcgcg gccttcgtta ttaattaccg
     9481 taccgtcgct ttcagcaaag ctggcagcgg aaagcaccag atgggcgttt tccatgatcg
     9541 ctgtgcgttg atggtcaacc accatcacca gcggcgcttt agccagcgca gcattcacgc
     9601 gggtagcaga agcgtgacga tgcagatcgt tttccagcac caccaccgcg tcggcgcgtc
     9661 cggtttccag ttcggttaac gcttcttcaa gcgaaccgcc acccataatg cccagcccca
     9721 tgctgttgac ggaacgggca atcatggtga taccgacgtc agcgccgcga cctttcaggg
     9781 cttttgcgac gttagccgcc gcctgaatca cctctaagct accggcgttc gtcccggaga
     9841 taatcaacgg tttcttcgca cccgccagtg cctgcacgat gacgtcgatt ttgctttgca
     9901 gctcaggttc aataccgtca accgctggcg cagagttatc cagcgcatgg gcgatggcaa
     9961 aacctaaacg cgcctgatct tcaaccggtg cgcggtaagt ccacgccgcg atatcatcca
    10021 gacgggtgtc atcaacgttg gtaacaaaca gcggatgctt agcacgttga ccgatattga
    10081 ggattgccgc aatctgccag tcagccactt tctgtgctgc cgccatttcg cgcgctttac
    10141 ctttcaccgc ctgacgcact gccagcgcga cgcgcgcgcc ggtctgggta acgtcttcgc
    10201 ccagcaccag taccgcatcg taagattcga tttcgcgcag agccggagta taaatgccgc
    10261 cttcacgcag cactttcagc gccagttgca gacgttcctg ctcaccgtga gcgataccgg
    10321 tgtagaagtt ttcttcgccc accagttcac gcagcgcaaa gttgctttcc acgctggcgc
    10381 gcggagaacc aatgccgatc actttcttcg actgacgcag aatatctgcc gcgccctgca
    10441 ttgcctgttc ggcgttgagg gtaatgaaat catcgccacg acgctgtact ggctgacgcg
    10501 gacgatcttt caggttgacg taaccataac cgaaacgacc acggtcacag aggaagtagt
    10561 ggtttaccgt accgttgtaa cggttttcga tacgacgcag ttcgccataa cgctcgccgg
    10621 ggctgatgtt acagccgatg gaacattgct ggcagatgct cggcgcaaac tgcatatccc
    10681 atttacggtt gtaacgctcg gagtgcgttt tgtcggtaaa tacgccggtc gggcaaattt
    10741 cgaccaggtt accggaaaat tcgctttcca gcgtgccgtc ttccgggcga ccgaagtaga
    10801 cgttgtcgtg cgcgccataa acgcccagat ctgtaccgtc agcgtaatct ttgtagtaac
    10861 gcacgcagcg gtagcaggcg atgcagcggt tcatttcgtg agagatgaac ggccccaaat
    10921 cctgattacg gtgggtacgt ttggtgaaac ggtagcgacg gaagctgtgt ccggtcatca
    10981 cggtcatatc ctgaagatgg cagttaccgc cttcttcaca taccggacag tcgtgcgggt
    11041 ggttggtcat taaccactcg accacacttt cacggaactg tttcgcttct tcgtcgtcaa
    11101 tggaaataaa ggtgccatcg gaagccggtg tcatacagga catcaccagg cgaccacgcg
    11161 tgtcttccgc gttttggtat tgcttcaccg cacactggcg gcaagcaccg acgcttccca
    11221 gcgccggatg ccagcaaaag taaggaatat caaggcccag agacagacaa gcttccagca
    11281 ggttgtccgc tccgttgacc tcgtattctt tgccgtctac atgaattgta gccattagca
    11341 tgcttccagt tttctcagtc agagactgag cgttaatcga aattcggtta ccagcgctct
    11401 ttcagcaggt tcggctgaat cccattaatc aaatgggtat tgctgaacgg ctgtttgatt
    11461 cccgcctcaa attcttcgcg gaaatatttg atggcgctct gtaacggctc cactgcacca
    11521 ggtgcgtggg cacagaaagt tttacccggc cctaagaatc gacacagttg ctcaagtgtt
    11581 tcgatatctc ccggctgacc ttcgccacgc tccagcgcac gcagaatttt cacgctccac
    11641 ggcaggccgt cgcggcacgg cgtacaccag ccgcaggact cacgggcgaa aaactcttcc
    11701 aggttacgca ccagcgacac catgttgatt tcatggtcaa ccgccatcgc cagcgccgta
    11761 cccagacggc ttcccgcttt accgatactt tcgaattcca tcggcaggtc aaggtgcgct
    11821 tcggtcagga agtcggtgcc cgcgccgcct ggctgccagg ctttgaattt cagaccgtca
    11881 cgcataccac cggcgtaatc ttcgaggatc tcgcgcgcgg tagtaccaaa cggcagttcc
    11941 cacagacccg gatttttcac ccgaccggag aagcccatca gcttggtgcc agcatcttta
    12001 cttttcgaga tgttctgata ccactccacg ccgttagcga ggatcgccgg aacgttacac
    12061 agggtttcga cgttgttgac acaggtcggt ttgccccata cgccggaggt tgccgggaag
    12121 gggggtttcg agcgtgggtt agcacgacgt ccttccaggg agttgattaa cgctgtttct
    12181 tccccgcaga tgtagcgccc tgccccggta tggacgaaca gttcgaagtc aaaacctgtt
    12241 cccataatgt ttttgccaag caatcccgct tcagtggctt cggcaatggc gcggcgcaga
    12301 tttactgccg cttcaatata ttcgccacgc aggaagatgt agccacggta agctttcagc
    12361 gcaaacgcgg agatgagcat gccttccacc agtaggtgcg gcagttgctc catcaacagg
    12421 cggtctttat aggtgcctgg ctccatttca tcggcgttac acagcaggta acggatgttc
    12481 atggattcgt ctttcggcat caggctccat ttcaggccgg tagaaaagcc cgcgccaccg
    12541 cgccctttca gaccagcgtc ttttacctga ttaacgattt cgtccggaga cagcccggtc
    12601 agcgccttac gcgccccttc gtaaccgttt ttgctgcggt attcgtccag ccacactggc
    12661 tgtttgtcat cgcgcagacg ccaggtcagc ggatgcgttt cgggagtacg gataatgttt
    12721 ttcatttata ccgctccagc agttcaggga tcgcttccgg ggtcagatgc gcgtgagtgt
    12781 cctcatcgat catcatgttt ggccctttat cacagttccc caggcagcaa gttggcagca
    12841 gcgtaaagcg gccatcaaat gtcgtttgcc ctggtttgat gttcagcttt ttctcgagcg
    12901 ccgcctgaat accctgatac ccgttgatat gacagaccac gctgtcgcaa taacggatca
    12961 catggcgacc aaccggctgg cggaagatct gactgtagaa cgttgccaca ccttcgacgt
    13021 cgcttgccgg aatacccagc acatcggcga tcgcgtggat cgcaccatcc ggcacccagc
    13081 cacgctgctt ctgaacgatt ttcagcgctt caatggacgc cgcacgcggg tcttcgtagt
    13141 ggtgcatctc gtgctcaatc gcttcacgct ctgccgcact cagctcaaaa gcctcggttt
    13201 gtggttgttg attctcgtgc ataattagcg gtccacatct gacataacaa aatcgatact
    13261 gcccagataa acaatcaggt cagacaccag gctgccgcgg atcgccgccg gaatttgctg
    13321 caaatgcgca taactcggcg tgcggatacg agtgcgataa ctcatggtgc taccgtcgct
    13381 ggtcaggtag taactgttga tccccttggt cgcctcaatc atctggaaag attcattggc
    13441 aggcatcacc ggcccccacg acacttgcag gaagtgggtg atcagggttt cgatatgttg
    13501 cagcgtgcgc tctttcggcg gtggcgtggt cagcgggtga tccgctttga acgggccttc
    13561 cggcatgttg ttgaggcact gctcaagaat gcgcagactc tggcgcagct cttccacttt
    13621 cagcattacg cgggtgtagc agtcagaaac accaccacca accgggattt caaagtcgaa
    13681 gttttcatag ccagaataag gacgcgcctt acgcacgtcg aagtcgatcc cggtagcacg
    13741 caggcccgcg ccagtggtgc cccactccag cgcctctttc gcgccatagg cggcaacgcc
    13801 ctgggaacga cctttcagaa tggtgttttg cagcgccgct ttctcgtaag acgccagacg
    13861 tttcggcatc cagtcgagga actcacgcag caggcgatcc cagccacgcg gcaggtcatg
    13921 cgctacgccg ccaatacgga accacgccgg gtgcatacgg aaacccgtga ttgcttccac
    13981 cagatcgtaa attttctgac gatcggtaaa ggcgaagaac acgggcgtca ttgcgccgac
    14041 gtcctgaata aaggtcgaga tatacagcag gtgactgttg atgcggaaca gttcggagag
    14101 cattacgcga atgacgttaa cgcgatccgg cacggtgatc ccggccagtt tctctaccgc
    14161 cagcacgtaa ggcatttcgt taacgcagcc gccgaggtat tcgatacggt cggtatacgg
    14221 aatgtagctg tgccaggact ggcgttcgcc cattttctcc gcaccacggt ggtggtagcc
    14281 gatgtctggt acgcagtcga caatctcttc gccatcgagc tgcaaaacga tacggaaagc
    14341 accgtgcgcc gacgggtggt tcggaccgag gttgaggaac atgaagtcct cgttttcggt
    14401 gccgcgcttc atcccccact cttccggttt gaaggtcagg gcttccatct ccagatcctg
    14461 tttggctttg gtcagctcaa acggcgagaa ttcggtagcg cgcgccgggt aatctttacg
    14521 cagcgggtga cctttccagg tttgcggcat catgatgcgg cgcaggttcg ggtgaccgtc
    14581 gaatgtaatg ccaaacagat cccaggtttc acgctcatac cagttagcgt tcgggaacag
    14641 tttggtgaag gtcggtacgt gcaggtcgtt ttctgccagc gccaccttca gcatgatgtc
    14701 gcggttacga tcaatagaaa tcagatggta gaaaacggaa aaatccgcgg caggtaaccc
    14761 ttcgcggtgt gtgcgcagac gttcgtccat gccgtgtaag tcaaacagca tgacgtaagg
    14821 tttcggcagt ttctttaaga aatcgccaac ttccagtaat tgttcacgct tgatccacac
    14881 aacgggaacc ccggtgcgag tcgcctgaac agtaaaggca tccggcccaa aacggttgcg
    14941 cagttcgcca attaccggat catcaagatg atcgcgggtc tgccaggcgg gttcttgcgc
    15001 ggttaagtcg gtcatattgt tcaccattgc aaatggtccg tggtgactgt cggcgcagta
    15061 agcttcgcga aatagattta ggaataagcg aagaaaaatc cctttgccga caggcgcaaa
    15121 ttaaatctcg tcaggtgtac gcaggttggt tacggcaatg cgttcaccgc gcttgcgttc
    15181 gcgctctgat tgcatattgg cgcgataaac gccctgatcg ccaaccaccc aggagagcgg
    15241 acgacgttct ttgccgatag attcctgcaa cagcatcagt gcctgcatgt acgcttcagg
    15301 acgcggcggg cagcccggga tatacacatc aaccgggatg aatttatcga cgccctgcac
    15361 aacggaataa atatcgtaca taccaccaga gttggcacag gcacccattg agataaccca
    15421 ttttggttcc agcatctggt catacagacg ctgaataacc ggtgccattt tggtaaagca
    15481 ggttcctgca accaccatca ggtcagcctg acgcggcgaa gcacgcaata cttctgcgcc
    15541 aaaacgcgcc acgtcatgca ccgcggtaaa cgaagtcacc atctcaacgt aacagcagga
    15601 aagaccgaag ttatacggcc aaattgagtt tttacgaccc cagttaacca tgtcattgag
    15661 cttgcccata aacacgtttt tgttaacttc ttgctccaga gggtcggtta cgatctcctg
    15721 cttttgcagg gggtaacggt cgttctcacc gttgggatct atgcgggtga gcgtataatc
    15781 catcttaatg cctcgcggtt agcgttgacg attagcgata ctgttcgttt ccgggttcat
    15841 acgctcgcgg cgtgaacgcg cgggcgtcca gtccagcgcg ccaatacgca ccagataaac
    15901 cagacctgcc agtaacacaa aaataaaaat tgcagcttcc acaaagccta cccagccgct
    15961 ttcgcggata gaggttgacc atgcgaacag atacagcgct tcaacgtcga agataacgaa
    16021 gaacatggcc accagataaa acttggcgga caggcgtaag cgggcggagc cgaccgagtc
    16081 gataccggat tcaaacggca cgtttttcga cctcgcgcgt gcgcgaccgc ctaaaaacca
    16141 accgcctacc agcatcaggc aacacaggcc aatggcaacg ataagaaaga tagcgaatgc
    16201 ccagtgatga gcgatgactt cagtggatgt tgacatactc attgcttact catcaaaagt
    16261 agcgccagat tcactgctct tcacggcaga tggacgccac atcgattcat ggggaggaat
    16321 aaaaaaaacc ttacaatcac tgtagaaatt cttttataca gctaattgat gtggtctttt
    16381 actcctttct ataacctttt gtcaacttta acaaaagttt cttcacatta gtttacataa
    16441 tatcaacacc attagcattt aatgcccttt caccccagat ccttgacgac tccaggataa
    16501 ttagatgttg ttgaatcgtg tccgttgtga agcaatggaa aaaatacggg tctattttga
    16561 caggaatttg tgtcgattcc tccccccaaa agagagtatt ttcttgatct gtgacacgct
    16621 tttgtcattc cataacaaaa acgcagcaac aaatttacgt attttttaac atcattgtag
    16681 caggtgattt ttttcaggcg attatttgtg tgtacggggc gtgaatctct ggcgattgaa
    16741 aaatgaacag ttttgtacgt tctgcacgat gtgaagaagg ttctttacta aaaaaaagcc
    16801 gctggggttt aaaacacccc cagcggctcg ttttttacac tattgtctca ggaattatct
    16861 atcgtccgtc gattactcga tatccctttc aatcaacaat gaatcatccc cttccggagc
    16921 agatattggg ctgtattgcc acggattatg gtagctctcc atcgcctgat agatcacctg
    16981 ggccagctca ttattactgg acgggtcata gcacagcaaa tattcggtat caggtagcgg
    17041 cggtaagcca tctactccac tcagcacacg caagtccgga ctcatcatct cgaccggcct
    17101 tgccgtcacg ccaagaccgg ctttcactgc cgcacgaacg gccggaagcg tcgaggcgac
    17161 ataagccagt cgccagggaa tatctgcttt attaagcgtc gccagcacca tatcgcgaaa
    17221 cggacttgga tcatccagca atacaagagg gatcggctca cctttttgca atacgtattc
    17281 cgctgcgcag taccagtgtg ttggcgaggt acgcaggttc aatgccttaa acgctgaagg
    17341 acgatgggtg gttaccatca aatccacttc ctgggattcc agcatttctg ccatataggc
    17401 attacgttta acgcggacat ccagcgctaa tttcggataa accgaactca cgcgatttaa
    17461 caggaaaggt aagatcgtat cggcagattc atctgaagca ccgatagtta acacgccctg
    17521 aagattactg aacattaatg atgagcaggc ctcatcatta aaacgcagga ttttcctggc
    17581 gtaaccaaga agttgaatgc catgttcagt taacagtttg ttgcgaccgt gacgagcgaa
    17641 cagttctttc ccaacgagtt gttccagacg ctgcatttgc tgacttacgg cggactgagt
    17701 acgacacaca gcggcagctg cggcagcaaa agtgttcaga tcggcaacag caacaaatgt
    17761 tctcagcaga tcgaggtcga ggttaattat cggacgattt gcacttatca tatattatca
    17821 cttactggcg gctcatactg agctggttaa tgctgtgcac acacaaacaa gcaattccat
    17881 ttgtaatgtg cctccctggc agtttcatcc ggaacccgga cgaaagtaaa aatgcatatg
    17941 agttgcacta aaaaagcgac tcacattgtt ccgttataat gcctgaagta gatcacagaa
    18001 tatatcttca gggatcgcat atctattaag ttactcactc ttttctattt atgacatgcg
    18061 cgtgtttgta taaatgtaaa tgtgagtcct tgttccactc tcgtgcagca tcgctggtca
    18121 tacgcgaaca cgtacaaaca gcaatggtga ggcggcatca agagcaggga tccgtcattt
    18181 atgcgagcat tttaccccaa aaacctttta tttataaggg tcattgcgaa ttatctga
//
LOCUS       DAPGOF010000094        18233 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.094, whole genome shotgun sequence.
ACCESSION   DAPGOF010000094 DAPGOF010000000
VERSION     DAPGOF010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.094"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            91..1311
                     /locus_tag="RHV13_002839"
                     /pseudo
     CDS             91..1311
                     /locus_tag="RHV13_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
     gene            1284..1913
                     /locus_tag="RHV13_002840"
     CDS             1284..1913
                     /locus_tag="RHV13_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HDV1242358.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1914..3074)
                     /gene="ybdL"
                     /locus_tag="RHV13_002841"
     CDS             complement(1914..3074)
                     /gene="ybdL"
                     /locus_tag="RHV13_002841"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HDV1242359.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIALKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     IADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTREHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3183..4271
                     /gene="hcxA"
                     /locus_tag="RHV13_002842"
     CDS             3183..4271
                     /gene="hcxA"
                     /locus_tag="RHV13_002842"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HDV1242360.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEVFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDALLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4281..4478)
                     /locus_tag="RHV13_002843"
     CDS             complement(4281..4478)
                     /locus_tag="RHV13_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HDV1242361.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4660..6765)
                     /gene="cstA"
                     /locus_tag="RHV13_002844"
     CDS             complement(4660..6765)
                     /gene="cstA"
                     /locus_tag="RHV13_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HDV1242362.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(6946..7359)
                     /gene="entH"
                     /locus_tag="RHV13_002845"
     CDS             complement(6946..7359)
                     /gene="entH"
                     /locus_tag="RHV13_002845"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HDV1242363.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7362..8108)
                     /gene="entA"
                     /locus_tag="RHV13_002846"
     CDS             complement(7362..8108)
                     /gene="entA"
                     /locus_tag="RHV13_002846"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HDV1242364.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8108..8965)
                     /gene="entB"
                     /locus_tag="RHV13_002847"
     CDS             complement(8108..8965)
                     /gene="entB"
                     /locus_tag="RHV13_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HDV1242365.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(8979..10589)
                     /gene="entE"
                     /locus_tag="RHV13_002848"
     CDS             complement(8979..10589)
                     /gene="entE"
                     /locus_tag="RHV13_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HDV1242366.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTCHAASDSIAVID
                     GERQLSYRELNQAANNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGE
                     HNLQDAINHPADGFTATPSPADEVVYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQINVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10599..11774)
                     /gene="entC"
                     /locus_tag="RHV13_002849"
     CDS             complement(10599..11774)
                     /gene="entC"
                     /locus_tag="RHV13_002849"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HDV1242367.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            11963..12919
                     /gene="fepB"
                     /locus_tag="RHV13_002850"
     CDS             11963..12919
                     /gene="fepB"
                     /locus_tag="RHV13_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HDV1242368.1"
                     /translation="MRLAPLYRNALLLTGLLFSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQALLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(12923..14173)
                     /gene="entS"
                     /locus_tag="RHV13_002851"
     CDS             complement(12923..14173)
                     /gene="entS"
                     /locus_tag="RHV13_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HDV1242369.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDG"
     gene            14284..15288
                     /gene="fepD"
                     /locus_tag="RHV13_002852"
     CDS             14284..15288
                     /gene="fepD"
                     /locus_tag="RHV13_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HDV1242370.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15285..16277
                     /gene="fepG"
                     /locus_tag="RHV13_002853"
     CDS             15285..16277
                     /gene="fepG"
                     /locus_tag="RHV13_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HDV1242371.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16274..17089
                     /gene="fepC"
                     /locus_tag="RHV13_002854"
     CDS             16274..17089
                     /gene="fepC"
                     /locus_tag="RHV13_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HDV1242372.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(17086..18219)
                     /gene="fepE"
                     /locus_tag="RHV13_002855"
     CDS             complement(17086..18219)
                     /gene="fepE"
                     /locus_tag="RHV13_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HDV1242373.1"
                     /translation="MSSLNIKQGSDAHFPDYPLASPSNNEIDLLNLISVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKMKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
BASE COUNT         4278 a         5171 c         4720 g         4064 t
ORIGIN      
        1 aaaataaaac actctgcttt tcagtgttta ctttataatt aacagtctta tctcactgat
       61 caccctgtaa cgttcagaga gcgtcttcag atgtctattt ataaaattcc tcttccgctc
      121 aatattctgg aagctgcacg agagcgtatt acctggacac tcaacaccct accccgcgtt
      181 tgtgtctcat tttctggcgg caaagactct ggtttaatgc tgcatctgac agccgaactt
      241 gcccgacaaa tgggcaaaaa aatctgcgtt ttgtttatcg actgggaggc gcaattctct
      301 tgcaccatta actatgttca gtccctgcgc gagttgtaca ccgatgtcat cgaagagttt
      361 tactgggttg cgctcccgct tacgacgcaa aattcccttt cacaatacca acccgaatgg
      421 cagtgctggg aacctgatgt cgaatgggtg cgtcagcccc cgcaagatgc gataaccgat
      481 cctgactttt tctcctttta ccagccaggc atgaccttcg aacaatttgt acgtgagttt
      541 gccgaatggt tttcacaaaa acgtccggcg gcgatgatga tcggcatccg tgcggatgag
      601 tcctacaacc gttttgtcgc catcgccagt ttaaataaac aacgttttgc cgacgataaa
      661 ccctggacta cggccgcacc aggcggtcat agctagtaca tttaccccat ttacgactgg
      721 aaagtggctg atatctggac ctggtatgca aatcatcaaa gtctctgcaa cccactgtat
      781 aacctaatgt atcaggcagg cgttcctctg cgtcatatgc gaatttgcga accttttggc
      841 ccggagcaac gacaaggatt atggctctat cacgttatcg aaccggatcg ctgggctgct
      901 atgtgcgcac gagtcagcgg ggtaaaaagt ggcggcattt acgccggaca tgacaatcat
      961 ttctatgggc atcggaaaat cctcaagcca gaacatttag actggcaaga atatgcatta
     1021 ttgctgctca atagcatgcc ggaaaaaaca gctgagcatt accgcaataa aatcgccatt
     1081 tatttgcact ggtatcagaa aaaaggcatc gaggttccac aaacccagca aggggacatt
     1141 ggcgcgaaag atatcccctc ctggcggcgg atatgcaaag ttttactcaa taacgattac
     1201 tggtgtcggg cattatcatt tagtcctacg aaagcgaaga actatcagcg ttataacgaa
     1261 cggataaaag gaaaacgtca ggaatggggg atactatgca acaacgatta acgcaggatt
     1321 taacgcaatt tctcgccagc ttgccggaag atgatcgcat caaagcgatt aatgaaatcc
     1381 gcatggcgat ccatcaggtt agcccctttc gtgaagagcc agtggattgc gttctgtggg
     1441 ttaaaaacag ccaacttatg cctaatgatt acaacccaaa caacgtggca ccacccgaaa
     1501 aaaaactgct gaaaaaatcc attgaaatag atggttttac ccaacccatt gtggtcacgc
     1561 atacggataa aaatgccctg gaaatcgtgg atggttttca ccgccatgaa attggtaaag
     1621 gctcatcatc attaaaatta cgcttaaaag gttatttgcc ggtgacctgt ctggaaggta
     1681 cgcgtaatca acgcatcgcc gcgactattc gtcataatcg cgctcgtgga cgccatcaaa
     1741 tcaccgccat gtcggaaatc gtccgtgaac ttagccagtt gggatgggac gataataaaa
     1801 ttggcaaaga gctgggaatg gacagcgacg aagtgctgcg cctgaagcaa attaacggtc
     1861 tgcaagagct gtttgccgac cgtcaatatt cccgcgcctg gactgtcaaa taactacagc
     1921 tggcgcagtc gttcagctgc tgccagcaac gtcgattcct tcttggcaaa acagagacga
     1981 atcagtttat gtgggaaggg atcggcgcaa aacaccgata gcggaatcgc cgccacgccg
     2041 tgctcccgcg tcagccactg gcaaaactca acatcatcca gggtagaaac cgcgctgtaa
     2101 tccaccagca aaaagtatgt gccttcgcac ggtaaaatct ccagccgact ttcatttaag
     2161 gcatttacca gaatatcgcg cttctggcga taaaagtcgg gtaacgcaag ataatgctca
     2221 ggttctgcac gtagcatatc cgcaattgcc agctgtgccg gggtatttac cgaaaaggtc
     2281 agatactgat gtaccttgcg aatttcggcg ctgatgggcg ctggcgcaac acaataaccc
     2341 actttccagc cggtcatatg ataggtcttg ccaaatgaag aaaccgccac tgcccgctca
     2401 cgcagctgcg gatgcgccag cacactggca tggccctgtt gtgaaaagtt gatgtgctcg
     2461 tagacttcat cgctaatgac aaaaatctcg tgcccggcga tcgcctgcca caaagcggcg
     2521 aaatcagcct gctgccagac agttgcactg gggttatgcg gagtgttgag gatcaccagt
     2581 ctggtgcgct cgcttaacaa tgcggcaaat tcctgccagt caacgcgaaa atgcggtggt
     2641 tgcagtgcca tacgcttcac tattccccca gaaagcgcga tggcgggggc gtaactgtca
     2701 tagctgggat caaaacaaat cacttcatcg ccattgcgca ccagtgcggt aatcgccgcg
     2761 tataacgctt ccgtcgcccc tgccgttacg gtgatatcgc tatcggcatc tggttgatag
     2821 ccatacaaac gttccgtttt cagagcaatc gcctcgcgca aggcctgcac gccggtcatg
     2881 ggcgcgtatt ggtttgcccc ctgggcaacg tggtacgcca gccgctcctg taaatagcgc
     2941 ggaccatcaa aatcaggaaa gccttgcgac aggttaatcg cctggtgttg ctgcgccagc
     3001 gcgctcatct gggtgaaaat agtggtgcca agttgtggaa gtttgctttg tggaatcaga
     3061 gggttatttg tcatttttat tgtacctgac gaagttgcgt gttgaaggca ctataacacg
     3121 atgctagtat ttggcaatca agacgtttag atgtctaaat aaaacaataa ggacaacaca
     3181 acatgcctca caatcctatc cgcgtggtcg tcggcccggc taactacttt tcacatccag
     3241 gaagtttcaa tcacctgcac gattttttca ctgatgaaca actttctcgc gctgtgtgga
     3301 tctacggcaa acgcgccatt gctgcggcgc aaaccaaact tccgccagcg tttggacttc
     3361 caggggcaaa gcatattttg tttcgtggtc attgcagcga aagcgatgta cagcaactgg
     3421 cggctgagtc cggtgacgat cgcagcgtgg tgattggcgt cggcggcggc gcactgctcg
     3481 acaccgcgaa agccctcgcc cgccgtctcg gtctgccatt tgttgccgct ccgacgatcg
     3541 ccgccacctg cgccgcctgg acaccgctct ccgtctggta taacgatgcc ggacaggcgc
     3601 tgcattatga ggttttcgac gacgccaatt ttatggtgct ggtggaacca gagattatcc
     3661 tcaatgcgcc gcaacaatat ctgctggcgg ggatcggtga cacgctggcg aaatggtatg
     3721 aagcggtggt gctggctccg caaccagaaa cgttgccgct aaccgtgcga ctggggatca
     3781 ataatgcgca ggccattcgc gacgccttgt taaacagtag cgaacaggcg ctgagcgatc
     3841 agcaaaatca acagttaacg caatcatttt gcgatgtggt ggatgctatt attgctggtg
     3901 gtgggatggt cggtggtctg ggcgatcgtt ttacgcgtgt ggcggcggct catgccgtgc
     3961 ataacggtct gaccgtgctg ccgcaaaccg agaagtttct tcacggcacc aaagtcgcct
     4021 acggaattct ggtgcaaagc gccttgctgg gtcaggatga tgtgctggcg caattaactg
     4081 gagcgtatca gcgttttcat ctgccgacta cgctggcgga gctggaagtg gatatcaata
     4141 atcaggcgga gatcgacaaa gtgattgccc acaccttgcg cccggtggag tccattcatt
     4201 acctgccagt cacgctgaca ccagatacgt tgcgtgcagc gttcgaaaaa gtggaatcgt
     4261 ttaaagcctg acgtgcaaat ttagcagcag cgcgcgccgc cttttccacc gtagcgcgcg
     4321 tcctgccgct cacggaaaaa ctcttcgtag gtcatcggcg tttgatcggg atggttaacc
     4381 cgcatatgtt cgacatagtt gtcgtaatca ggcataccaa tcatcagctt cgccgcctga
     4441 cctaaatatt ttccggcttt tgccagtgaa tcaaacatct tggcctccgc taaccatgtt
     4501 gctgaaagag tgccggatgc ggcgtgaacg ccttatccgg cctacaaatg ctatcaacgc
     4561 gctcggtcag tcccctcgcc ccatcgggga gagggttagg gtgaggggga accctcggca
     4621 ctatgccaac attcgccaac atcccccctc actctgactt tagtgcgcgc cttttgcctg
     4681 cgccacgatc tcctcgacat tttccggcat tggctcatac ggcgtttctt tcgccgttgg
     4741 cttcggctct ttcaatgccg ccagtgccgt cttaatcgag aacagtgcca gaaccaccac
     4801 gaccaccata aagaagatgg ttaacccggc atccagacgg ttgttgaaca ccagttgcgc
     4861 cagctgtgac tcagtatact gcgacggaat attgccgctg tcgatcattg cctggaactt
     4921 gttagcaatg gccaggaagc cgactttcgc atccgggcta aacgctttct gccagcctgc
     4981 ggtcagggta caaatcagca gccaggccgt tggtaccagc gccacccagg cgtaacgttg
     5041 acgtttcatt ttgaacagca ccacggcaca gagcatcagc gccatccctg ccagcatctg
     5101 gttggcaata ccaaacagcg gccacagagt gttaataccg cccagcggat cgaccacacc
     5161 ctgatgcagg aagtagcccc acgctagcac gcacagcgcc gttgccagca ggttagcagg
     5221 cagtgaatcg gtccgtttca ggcccggaga aaccacgccc agcagatcct gcaacataaa
     5281 gcgcgcagca cgcgtacccg catctaccgc cgtcagaata aacagagctt caaacagaat
     5341 ggcgaagtga taccagaacg ccacatccat cataccaccc agcgcaccgt gcaggatgta
     5401 agccatcccc accgccaacg tcggcgcacc gcccgcacgg gaaatgatcg actgttcacc
     5461 cacttcgctg gcaatctggt ttagcgtatc cggggtaatc gcaaagcccc agctactcac
     5521 cacttgcgcc gcagaagcga ccacatccgc cgtccctgcc ggagccagca ccgccatcgg
     5581 gctgttcatg gcgaaataga cgcccggatc gatgatacag gcggaaacca gtgccataat
     5641 cgccacgaag gattccatca acatcccgcc gtagccgata aagcacgcct gcccttcgtt
     5701 cgccagcatc ttcggcgtgg taccggaaga gatcagcgca tggaagccag acaccgcacc
     5761 acaggcgatg gtgataaaca ggaacgggaa caggttaccg gtccataccg ggccagtgcc
     5821 atcgacaaat ttggtcagcg caggcatggt cagcgtcggg cgcataatca aaatgcctac
     5881 cgccagaccg acgatcgtcc cgattttcag gaaggtagag aggtagtcac gcggagccaa
     5941 cagcaaccac accggcagca ccgccgccac aaaaccgtaa cccaccagca tccaggtcag
     6001 ctgcacgccg gtaaagtcaa agtacggtgc ccaggtcgga ctttctgcca cccagccgcc
     6061 agaaataatg gcgaaaatca ggaataccag accgatgacc gacacttcac caatacgccc
     6121 cggacgcaga tagcgcaggt agattcccat aaacagcgcc agcggaatgg tgaacgcaac
     6181 ggtatacgtt ccccacgggc tatgagtcag ggctttcacc acgatcattg ccagtactgc
     6241 aaggataatg accatgatca taaagcaggc caccagcgcg atcaccccgg cggttggccc
     6301 catctcttct ttgaccagct cacccagcga gcgaccgtcg cggcgcgtgg aaacaaacag
     6361 caccatgaaa tcctgcaccg caccggcgag aaccaccccg gcgagcagcc agatcatccc
     6421 cggcaggtaa cccatttgcg ccgccagtac cggccccacc agcggacctg ctccggcaat
     6481 ggccgcaaaa tggtgaccga acagcacttt cttgtccgtc ggcacatagt ccagcccgtc
     6541 gttatggcgc acggctggcg tcatacgcgt cgggtcaacc gccagcacat ttttggcgat
     6601 atacaggcca taaaaacggt aagcgatcag atagatacag accgacgcca ccacaatcca
     6661 cagcgcgttg atctgttccc cacgatttaa agcaatgtat cccagagcaa atgctcccat
     6721 tacagagagc actgtccaga cgaggtattt ccctgatttg ttcatagttg ttatccgtgt
     6781 gcgtgtccat agagatgtta cattttgttt ctataacatc tttgcgcaat ttaacaacac
     6841 agcaaccatg taaatcggag ttgaaaccaa tatttaactt aacaatgtta actcgatcac
     6901 tccgttaacc gagttttcct gcaatctcat ataattctgt cgggttcatc ccaaaactgc
     6961 cgtacccagc cgacaagtgc agcaacgccg cccctgttca tcgaaaacga cgatttccca
     7021 gctctgattt tgccgcccaa gatgcagcgg ctggcagacg ccgcgtacct tgccttcaga
     7081 caccgggcga tgatgggtgg cgttaagttc cgtgcccacc acacactgcc cgtcacgggt
     7141 catcataaat ccggccatcg atcccagcgt ttccgccagc gccgccgacg cgccgccatg
     7201 cagcaggcca aacggctgat gagtacgggt atcaaccggc atttcggctt ccagcacatc
     7261 atcgcccaga cgggtataca caattcccag atgcgccacc attgtgttat cgctggtggc
     7321 gttcagttcg tcgagcgtta aatggcgttt ccagatcatg cttatgcccc cagcgttgag
     7381 ccgccatcga ccacaatatc ctgtagggtg atatggctgg cgaggtcaga ggcgaggaac
     7441 aaaatcgtgt tggcgatctc ttgtggacgg gcgattttcc ccagcggaat gccgagttta
     7501 aactgctcgc caaagccgcg aatacgctgt tcttcggcgt catcgctcac ccacagcgtg
     7561 cgttgcatat cggtgtcggt ggaacccggc gaaaccacat tacagcgcac gccgctaccc
     7621 gccagttcca gcccgacgct caacgccagg cttttcagcg ccgctttcga tgcgccataa
     7681 gcactcatac caatacgcgg cgtgtgtgcg gcgtcggacg ccacagtgac aatcgccccg
     7741 ccccgctgac ggcgaaactg gttcatggtt tgctggaaca ggttaaacgc accgccgaca
     7801 ttaaccgcaa aagtctgctg ccagtcctcc ttactgagct gatcggtcgc gcccatgcgc
     7861 aaaattcccg ccgcattgac cagcacgtcc agtcgctccg tttcagctaa cagtcgctga
     7921 catacctgcg ccacctgcgc agcatcggca acatccatta cttcggtcgc aaagggatat
     7981 tgctcctgag cgaacgcttg atcaaaacct gtaactttcg ctcccgcttc aacaaacgcc
     8041 agcgccgtgg cgtagccgat acctttcccc gcgccggtta cccagacatt tttaccgctg
     8101 aaatccatta tttcacctcg cgggagagta gcttccacca ggcgtcgatg gtcgggtttt
     8161 tcgccaacat gacaaagtcg atatcgccat gcactttgcg ccagcgcgcc gccagcgcca
     8221 tcatgcgcac cgaatccaga ccgtagtcga tcaggttgtc gtcatcgaac ggttcatcgg
     8281 actcgtccag caacggcagg atcacctcac gcagcgccgc tttgctggcg gggataggtg
     8341 ctggcagtaa ttcttcggtc atcaccaccc ggccagaacg tccggccaca tatttcagcg
     8401 acatcaaatg ctcgtcacgg ctgaaatcgg ccagcgcatc cgccaccata aacggtttaa
     8461 tatcgcgcat aaatgcgtcg gttgcggtgg tcatacagcc aatgtgggca tataccccgg
     8521 taataatcag ctggttacgt ccactctctt tgagcatttg ctccagcgga gaacgatgaa
     8581 acgcgctgta gcgccacttc accagcaccg tgtcgtcggc atctggcgtc agtcgatcca
     8641 ccaccttttg ctgttccggc gaacgggtca ggcccggccc ccacatatca ttcaacagcg
     8701 cccgatcttc atcgctctgc tctttcggct gggcggtgta ataaaccggg atattgtgct
     8761 gtttgcagta gtcgcgcagc gcagcaatat tcgcgatcac ctgctccatc atcgggcagt
     8821 tctcgcccca gaagctgaca aaatagtcct gcatatcatg gattaacaac gcggcacgtt
     8881 gcggttcaaa ggcccagtca actttattct gcggaatatc gtgagactcc ggcagtgcgt
     8941 aagcctgtaa ttttggaata gccatcgtgt tctctccttc aggctgatgc gcgtgacgcc
     9001 agccactgac gtaattgttt tttatcgact ttcccgaccg ccgtcagcgg aagtgaatcc
     9061 acacactcca cgcgatccgg taatttaaat tcggcaatac cctgttcacg caggaaacga
     9121 cgcacctgca ccgcgcgaag cggctctttt accaccagat aagcacagct tttttcgccc
     9181 atcagctcat cttccatact gaccagtgcg gcgtagatca ccgccggatg gcgcagtaac
     9241 aggttttcga tctcttcggc agcgatcttc tcgccgccac ggttgatctg atctttctcg
     9301 cgcccctgca cggtgatgta accctctgga tcaatagaga tcagatcgcc ggaacagtaa
     9361 aaaccgttgg catcaaaggc gctggcattg tgctgcgggc ttttgtaata gccacggaag
     9421 gtatacggcc cgcgcgtcat cagtcgtccg acttcccctt gcggcagtgg atttccttcg
     9481 gcatcggcaa cccatacttc gtcatccgga cacattgggt aaccctgggt atggataatt
     9541 ttctccgcgc tatcatcaag acgggtatag ttcaccagcc cttccgccat accaaacacc
     9601 tgctgcaact ggcaaccaat ctcagcggga atacgcgccg caagcgtggc agaaagacgt
     9661 gcgccaccga cctgtaacag tttcagcgag gcaagctgcg cccggctttc gccttcggtc
     9721 agcgcctgca accacaggct gactgccggc ggcaccagcg cggtaacgtt aatctgatgt
     9781 ttttcaatca atgggaagca aagcgtggcg ctgggatcgg ccgccagaac aaccgttcct
     9841 ccggcaagaa agacgcccag cgatcccggc gaactcatgg cgtagttatg agccgccggg
     9901 atcgcgcaca ggtagcgtgt ctgttgtgtg aactgacaaa tctcgacgct acgacgcacg
     9961 ctgtagtagt agtcgttatg agtgcgcggg atcagcttcg gtgtaccggt ggtgccgccg
    10021 gaaagctgga aatagaccac ttcatcagca ggtgacggcg tagcagtaaa accgtctgcc
    10081 ggatggttaa tcgcatcctg caagttatgc tcaccgctgt cgttgagtag ctgcaccacg
    10141 cgaatggaag aatgctctgc aacaaatgtg ttgaggaaat catccccgct aaacagcgca
    10201 tgttggcgat cggcaatcag caatgcgggt tcaatctggc tggcataggc gttcagttca
    10261 ctacgctgat ggctgaacaa cgccagcacc ggcgcaacgc ccagtttcag cagtgcgaaa
    10321 aaggtaatgt aaagttcagc gacgttaccc agttgtacca gcgcggtttc accaggttta
    10381 atgccctgac ggcgtaaact acacgcgagg ttattcgccg cctgattcag ctcccggtaa
    10441 ctcaactgtc gctcgccgtc gataaccgcg atgctgtcac tcgcagcgtg gcaagtcaga
    10501 atgtcggtca gcggcaaatc ctgccagtag cctttttccc gatagcgacg ggcaaactct
    10561 tccggccagc gggtgaatgg aatgctcatc ctcgctcctt aatgcaatcc aaaaacgttc
    10621 aacatggtag aaagtttgac gcctgtttcg cgccactcac ccaacggtga cgacgcaggc
    10681 acaatccccg ctccggcaaa cagacgcacc tgattttccc gcagcttcgc gcagcggatg
    10741 gtcaccaccc attcgccgtt accttcgctg tcacaccaac ccacaatgcc gccaaacagt
    10801 tcgcggtcga acggctccag ttcagcaata acctgggtcg cggcctgatg tgggaaaccg
    10861 ctcagcgcgg gggtcggatg cagcagacag gccagagtca gtgcgttttc ttgcgaattc
    10921 gctttacctt caaagggagt tgcgagatgc cacagcgtcg gcgtggtaat caattgtgga
    10981 gaggagggaa cgtgtaactc actactgcgt tcgcgcagta cctctttcat cgcctgagtc
    11041 accagttcat gttcatggcg atctttttct gacgccagca gacgattacc cgcttcgcga
    11101 tcgagcactt catccggctg acgacgtgcg gaaccggcta acggaatgga gctaaaacgc
    11161 tcaccgtctt tacgtagcag cagttccggg ctggccccca gcagaacgcc accatcagcc
    11221 agcgggacat ggaagttgta actaaccggg ttttgcgcaa tcaaccgttc cagcaatacg
    11281 ccactatcaa tggcggcgtc agtggtgata tcaatcaatc gtgacaacac cactttgtcg
    11341 acctgcggcg tggcggtaag tgcggcggcg cgggcaacca tctgttcaaa cgtggtttgt
    11401 tcaggaattg cctggcgttc caccacgttc agcgactggc tgcgggtgaa acggcgtgcg
    11461 gaagcttgtt tttcctgacg ggagaacgac tgccaggatt caggaatata cagcgacgaa
    11521 ggctgacgtg gatcgaaagg aatcgccccg accatcaccg gatttttgat gccctgcgct
    11581 ttggcatcgg caaacagcgc ggcgagtttt tgctggaagg gactgtcggg cgaatccccg
    11641 ttcacagccg gttcatcgaa gcgggcgaaa catcctgacg tcgtaaaact gcggtacggc
    11701 gacataaaga aaaagcgatt gggcgcaagt gttgccatgg tctgctgtac ttcctcagcc
    11761 agtgacgtat ccatatcatc ctccacaaaa tgataaaggc tttaataatg attatcattt
    11821 atattttcgg tcgctaacct aaaagcaaac gcgcactatg tcaactcttg aggtaacgca
    11881 atctgcaact cggggttgca tctggttgtg gttataatga aaatgagaag cattaacttc
    11941 attaataaca ggaagctgat ttgtgagact cgccccgctc taccgcaatg cccttctatt
    12001 aacaggactt ttgttttcag gaatagccgc agttcaggcc gccgactggc cgcgtcagat
    12061 tactgacagc cgtggcactc ataccctgga aagccagccg cagcgtattg tttccaccag
    12121 cgtcaccctg accggctcac tgctggcgat tgatgctccg gtgatcgcca gcggcgcgac
    12181 cacgccgaat aaccgcgtcg cggatgacca gggcttttta cgccagtgga gcaaggtggc
    12241 gaaagaacgc aaactgcaac ggctctatat cggcgaaccg agcgccgaag ccgttgccgc
    12301 gcaaatgccg gatctgattt taattagcgc aaccggcggg gattcggcgc tggcgctgta
    12361 tgatcagctt tccaccatcg ccccgacatt aatcatcaat tacgacgaca aaagctggca
    12421 ggcgctgtta acgcaacttg gcgaaattac cgggcatgag aaacaagcgg cagagcggat
    12481 tgcgcagttt gataagcaac tggcggcggc gaaagagcaa atcaaattac cgccgcagcc
    12541 ggtcactgcc attgtctata ctgccgctgc acacagtgcc aatctctgga cgccggaatc
    12601 agcacaaggg cagatgctgg aacaactcgg ctttacgctg gcgaagttac ccgcagggtt
    12661 aaatgccagc caaagccagg gtaaacgcca tgacatcatt cagcttggtg gggaaaatct
    12721 ggctgcaggg ttaaatggcg agtcactatt cctgttcgcc ggtgatcaga aagatgccga
    12781 tgctatttat gctaatccgc tgctcgcgca cctgcctgca gtacaaaaca agcaggttta
    12841 tgcgctggga accgagacgt tccgtctgga ttactacagc gccatgcaag tgctggaacg
    12901 acttaaggcg ctgttttaag cattaaccgt cagacgctgt cacctgcggc ggcgtctggc
    12961 gaaaacgtcg caactccacc agcaccagca ataacaacac gccgataatc aataaaccaa
    13021 aaccgctcgc gcttgcggaa gcaaccggtg tcatcatcgc tcccaaacca cccagcagcg
    13081 ccgcgcctat ggcatcgccc gtcacgtttt gtgccgtcca caaaccgtta atccgcccta
    13141 acatcgcttc cggggtttgc gtttgcagca ttgtgtattg cagcaacgag ctgaccgcac
    13201 tcaaccagcc gaacagcgcc agacaaacca cgcctaaaat ccacatcggc atcaggccaa
    13261 acagaccaat ggcgaggaac gatcccagcg tggagagcag catcaataac cctggtcgag
    13321 cgctatgcgc cagcttcccg ctggttaacg cgccaatagc cgcgccgagc gggatcgccg
    13381 cgtagagaaa accaatctgt gccgctgaca tctgccagtt gtcagccagc gccggataca
    13441 gtacccgcac cgcgctcgcc atcgtcaata aaccacccag cagcgcaatc cctcctacca
    13501 gcgggctggc gagcagaaaa cgaaatcctg ccagtaatga tttcaacgga tgctcacgcg
    13561 gctgcggtgg cggtggcagt gccggaaggc ttaacaacgg tagcaaggta ataaacgtgc
    13621 ccgccgccgc cagcccgtag ttccaggcca caccgccagt cgccagcagt aaaccgccaa
    13681 tcatgggcga aatcaccgac cccagacgca cggtcaacat ggtgatcgcc ccggcctgca
    13741 ttaagttttc acgccctacc agtgctggtg tcgccgccaa tagcgccgta acgccaagcg
    13801 atgcgaaaaa accatcccat aaaccaagta aatagattgc cagcaatgac ggctccggca
    13861 gcagcgcgtt caggcacagt ccaatgaagc caatgccaca ggtgccgcgc gccagcaaaa
    13921 tcactttttt gcgctcatag cgatccgcca gcacaccgcc gaccatcagg ccaacaaaca
    13981 tcgcgccgcc ggtcagcgtc accgaaagcc ccacctgcca ggtggaatgt gtcatcatct
    14041 ggatctgcac cggcaccgcg acgccgagca aacccagaga cacaattgag ataaaacgag
    14101 cgaggaatac tgcgcgaaac gccgggtgcg ttttcaacag gctgaggtta agcagccagg
    14161 attgtttatt cattacaatg ccttgccatc aaatatttta aatacctatt cttaggctgc
    14221 acatgctaac atatccaaat aagatcgata acgataatta atttcattat catggaagtt
    14281 cgtatgtctg gttctgttgc cgtgacacgc gccattgccg tgcccggatt gctgttatta
    14341 ctgattatcg cgacggcatt aagtctgctc attggggcaa aatcactccc cgcttccgta
    14401 gtgctggagg ccttctccgg cacctgccag agcgccgact gcaccatcgt gctcgacgcg
    14461 cggctgccgc gtacccttgc cggtttactg gcaggcggcg cgcttggcct tgccggggcg
    14521 ttaatgcaaa ccctcacccg aaacccactt gccgaccccg gcttgcttgg cgtgaacgcc
    14581 ggagccagct ttgccattgt gctaggtgcg gcgctgtttg gttactcttc cgcacaggaa
    14641 caactggcga tggccttcgc cggggcgctg gtggcctcgt tgattgttgc ctttaccggc
    14701 agccagggcg gcgggcagtt aagtccggtg cgtttaaccc tggcgggcgt ggcgctggct
    14761 gcggtgcttg aagggctgac cagcggcatc gccctgctta atcctgacgt ctacgatcaa
    14821 ctgcgtttct ggcaagccgg ttcgctggat attcgtaatc tgcacacctt aaaagtggtg
    14881 ctgatcccgg tgctgatcgc cggagcaact gcgctattac tgagtcgcgc gctgaacagt
    14941 ttaagccttg gcagcgacac cgcgacggcg ctgggcagtc gcgtggcgcg cacacagttg
    15001 attggtctgc tggcgattac cgtgctttgt ggtagtgcga cggcggtggt tggtccaatt
    15061 gcctttattg gcctgatgat gccgcacatg gcgcgctggc tggtaggtgc cgatcatcgc
    15121 tggtcgctgc ccgtcacgct actcgctacc cctgccctgc tgctgtttgc cgatatcatc
    15181 gggcgggtga ttgtgcccgg cgagctgcgc gtttccgtgg tcagtgcgtt tattggcgcg
    15241 ccggtgctaa tcttcctcgt gcgacgtaaa acgcgaggtg gtgcatgatt tacgtctctc
    15301 gccgattact catcacctgt ttgctgctga tttccgcctg tgtggttgca ggtatctggg
    15361 gattacgcag cggtgccgtc acgctggaga tctcgcaggt attcgccgcg ctgatgggcg
    15421 atgcgccgcg cagtatgacg atggtggtca ccgaatggcg tttaccacgc gtgctgatgg
    15481 cgctgttgat tggcgcagca ctgggcgtca gtggcgcgat ttttcagtcg ctgatgcgta
    15541 acccgctcgg cagccctgac gtaatgggct ttaacaccgg ggcgtggagc ggcgtgctgg
    15601 tggcgatggt gctgtttggt caggatctga cggctatcgc gctggcagca atggtgggcg
    15661 gcattatcac ttcgctgttg gtctggctgc tcgcctggcg taacggtatc gacacctttc
    15721 ggttgattat tatcggtatc ggcgttcgcg ccatgctggt ggcctttaat acctggctgt
    15781 tgctgaaagc gtctttagaa acggcgctaa cagcgggttt gtggaatgcc ggatcgctca
    15841 acggcctgac gtgggcaaaa acctcgcctt ccgcacccat cattatattg atgctcattg
    15901 ccgccgcctt actggtacgc cggatgcgct tgctggaaat gggcgatgat accgcgtgtg
    15961 cgctgggcgt cagtgtcgaa cgttcgcgtc tgttaatgat gctggttgca gtggtgctta
    16021 ccgctgcggc aacagcgctt gccgggccga tttcctttat tgctttagtc gcgccgcaca
    16081 ttgcccgacg cattagcggc accgctcgct gggggctaac ccaggcggcg ctatgcgggg
    16141 cgctgttact gctggcggcc gatctctgcg cccaacaact gtttatgccg tatcaacttc
    16201 cggttggcgt cgttaccgtc agcctcggcg gtatttacct tatcgtcttg ttaattcagg
    16261 agtctcgcaa aaaatgaccg aatctgtagc ccgtttgcgc ggcgaacagt taaccctggg
    16321 atatggcaaa tataccgttg cggaaaatct gactgtagaa atacctgatg gtcacttcac
    16381 ggcaattatc gggccaaatg gctgcggtaa atccacgtta ctgcgtacct taagccgcct
    16441 gatgacgcct gctcatgggc atgtctggct ggatggcgag cacattcaac attacgcaag
    16501 taaagaagtg gcccggcgga ttggtctgtt ggcgcagaat gctaccacgc cgggagatat
    16561 caccgtgcag gagctggtgg cgcgtgggcg ttatccgcat caaccgctgt ttacccgctg
    16621 gcgcaaagag gatgaagaag cggtaacgaa agcgatgcag gccacgggaa taactcatct
    16681 ggcagatcaa agcgtggata ccctttccgg tgggcaacgc cagcgagcgt ggatcgcgat
    16741 ggtgctggcc caggaaacgg caattatgct gctcgacgaa cccacgacct ggctggatat
    16801 cagtcatcag attgatttgc tggaactgtt aagcgaactg aaccgcgaga aaggctatac
    16861 cctggcggcg gtgctgcacg atcttaatca ggcctgtcgt tacgccagcc atttgattgc
    16921 attgcgggaa gggaaaattg ttgctcaggg agcgccgaag gagattgtca ctgctgaact
    16981 gattgagcgc atttatggtc tgcgctgcat gataattgac gacccggtag ccggaacgcc
    17041 gcttgtggtg ccgctcggac gaacggcacc ttcaaccgca aatagttaaa ctaagtggtc
    17101 tgccatcatg gcatcctgct ttctggatgc catcgcatag cgcaataaca cgctaccaca
    17161 agccaccatc ccgccgatca gcgcggaaag gatcacaata atcgccttac ccggaccgtc
    17221 ttttttcact ggcaatgacg ggcttaactg atatttaaac ggcgtaaaat tcacatcgtt
    17281 gatatttgct tttgttaact gctcaacaag atactgacga ttacgtaatt caccgttcag
    17341 ttccgcaacg tcagtgaccg ctttttcaat ttccagtttg cgttcaatac cgtctgcgcc
    17401 gagagaaata gagaaatcgg ggtcatcttt aacggcctga ccattactgt aaacgggctt
    17461 tttaattcct gccgcgttgg caatgtcgag tgaataattg aggcgctgaa tgtttgcatc
    17521 aagttgattt ttcattttaa tgcggtcctg agccagtttt tctttttcaa actgggtttt
    17581 gatctccagt ttattacgga cgttttctat cgactctttc accaccaacg cagagatata
    17641 atcgatatac ccgctcaaaa cggtctgcgc ctcttcactg gttggcgcgg taaaacttag
    17701 cgtccaggag gtatacagtg acggttcatc ttttttctta ctggcattgt catcaaccgc
    17761 tttcattttt tcgctcaatg cgacaattgc gcgatgcaaa tccagttcgt cgattttcgc
    17821 ctcttttaat tggtccatca cataaggtga tgaacgcagg tactcttcca gcaagctaac
    17881 cgactgaaac ttcttgataa acaggttaaa tgcttctgta cgatcaattt tgatatccag
    17941 atccagcaca cgaagcttag tgaatgtttt ctccaactct tgccactgaa caggttctgg
    18001 aggcgtgaca accgccgcgc tggtccattt ttgcggcagg atgaaagaga tcagcaagcc
    18061 tgcgcaggca aacgcaaaaa cgaccgccat gaccgttttt ttggcccgcc ataaaactga
    18121 gattagatta agtaaatcaa tttcattatt actgggcgac gccagaggat aatcgggaaa
    18181 atgagcgtca cttccctgtt taatattcag tgatgacatg gggtataacc tga
//
LOCUS       DAPGOF010000095        18089 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.095, whole genome shotgun sequence.
ACCESSION   DAPGOF010000095 DAPGOF010000000
VERSION     DAPGOF010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18089
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.095"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(139..786)
                     /gene="ychE"
                     /locus_tag="RHV13_002856"
     CDS             complement(139..786)
                     /gene="ychE"
                     /locus_tag="RHV13_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HDV1242374.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            1263..3938
                     /gene="adhE"
                     /locus_tag="RHV13_002857"
                     /gene_synonym="adhC"
     CDS             1263..3938
                     /gene="adhE"
                     /locus_tag="RHV13_002857"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDV1242375.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            complement(4239..4856)
                     /gene="tdk"
                     /locus_tag="RHV13_002858"
     CDS             complement(4239..4856)
                     /gene="tdk"
                     /locus_tag="RHV13_002858"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDV1242376.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            5461..5874
                     /gene="hns"
                     /locus_tag="RHV13_002859"
     CDS             5461..5874
                     /gene="hns"
                     /locus_tag="RHV13_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HDV1242377.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(6018..6926)
                     /gene="galU"
                     /locus_tag="RHV13_002860"
     CDS             complement(6018..6926)
                     /gene="galU"
                     /locus_tag="RHV13_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDV1242378.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(7128..8141)
                     /gene="rssB"
                     /locus_tag="RHV13_002861"
     CDS             complement(7128..8141)
                     /gene="rssB"
                     /locus_tag="RHV13_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HDV1242379.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(8233..9138)
                     /gene="rssA"
                     /locus_tag="RHV13_002862"
     CDS             complement(8233..9138)
                     /gene="rssA"
                     /locus_tag="RHV13_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HDV1242380.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAARQLAVEKKMDELLPLVRTNI"
     gene            9251..9709
                     /locus_tag="RHV13_002863"
     CDS             9251..9709
                     /locus_tag="RHV13_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HDV1242381.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELIAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTCPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            9759..10601
                     /gene="purU"
                     /locus_tag="RHV13_002864"
     CDS             9759..10601
                     /gene="purU"
                     /locus_tag="RHV13_002864"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HDV1242382.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVSRFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            10761..10845
                     /locus_tag="RHV13_002865"
     tRNA            10761..10845
                     /locus_tag="RHV13_002865"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10795..10797,aa:Tyr,seq:gta)
     gene            10892..11023
                     /locus_tag="RHV13_002866"
     ncRNA           10892..11023
                     /ncRNA_class="other"
                     /locus_tag="RHV13_002866"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(11207..11884)
                     /gene="narI"
                     /locus_tag="RHV13_002867"
     CDS             complement(11207..11884)
                     /gene="narI"
                     /locus_tag="RHV13_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HDV1242383.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(11884..12594)
                     /gene="narJ"
                     /locus_tag="RHV13_002868"
     CDS             complement(11884..12594)
                     /gene="narJ"
                     /locus_tag="RHV13_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HDV1242384.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLPKEDAHALG
                     IFLRDLTTMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(12591..14129)
                     /gene="narH"
                     /locus_tag="RHV13_002869"
     CDS             complement(12591..14129)
                     /gene="narH"
                     /locus_tag="RHV13_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HDV1242385.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIECAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(14126..17869)
                     /locus_tag="RHV13_002870"
     CDS             complement(14126..17869)
                     /locus_tag="RHV13_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HDV1242386.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         4562 a         4724 c         4318 g         4485 t
ORIGIN      
        1 aggaaatcct tataactaac tgtaatttga gaagaatatt tttacctatt ccttttgctt
       61 ttgataactg cgcaggaaca aagtaaaaat aatgacgaag taaacggagc atggcagctc
      121 cgtttcattt gagaggaatt aattaagcag gccggggaaa atccccttaa taccagtaac
      181 gataaattca atccccaatg ccatcagcaa tagccccata atacgcgtaa tcacgttgat
      241 gccggtctgg cgtaaaaccc gtaccagcca cggtgccatg cggaacaatc cccagcaaca
      301 taaagcgaac aatgcaatag ccacaaagaa accaaacaga tagctaatgc tgtgataacg
      361 cgtaccccag acgatggtag aactgatcgc ccctggcccc gccatcaacg gcaacgccag
      421 tggcaccaca ccaatgcttt cacgtaccgc ggtttctgat ttttcttgct tgttctgttt
      481 atcctcgcca agcttgccgc tgatcattga catcgctatt gtcaccacca ggataccccc
      541 ggcgatacgg aacgaatcaa ttgatatacc aaaaagttgt agaatcgtgt cgccgagaaa
      601 aagcgagatc cacaagataa tggccacaga caggttggct gtaaggttag ttttgtttcg
      661 cgctgccgct gtctgataac tggtcatgct gataaagacg ggaataatcc ctaccgggtt
      721 gaccagcgca aataacccga tgaaaaattt gaagtaaacg ggaaaatcaa aaagggtctg
      781 aatcacggtt agctccgaag caaaagccgg ataatgttag ccataaataa ggttgaaaag
      841 acgcgctgac aatacgcctt ttgacagcat ttttcacctc ctaactactt aaaattgcta
      901 tcattcgtta ttgttatcta gttgtacaaa acatgctaat gtagccacca aatcatacta
      961 caatttatta actgttagct ataatggcga aaagcgatgc tgaaaggtgt cagctttgca
     1021 aaaatttgat ttggatcacg taatcagtac ccagaagtga gtaatcttgc ttacgccacc
     1081 tggaagtgac gcattagaga taataactct aatgtttaaa ctcttttagt aaatcacagt
     1141 gagtgtgagc gcgagtaagc ttttgatttt catcggttaa gcaaatcatc accgcactga
     1201 ctatactctc gtattcgagc agatgattta ctaaaaaagt ttaacattat caggagagca
     1261 ttatggctgt tactaatgtc gctgaactta acgcactcgt agagcgtgta aaaaaagccc
     1321 agcgtgaata tgccagtttc actcaagagc aagtagacaa aatcttccgc gccgccgctc
     1381 tggctgctgc agatgctcga atcccactcg cgaaaatggc cgttgccgaa tccggcatgg
     1441 gtatcgtcga agataaagtg atcaaaaacc actttgcttc tgaatatatc tacaacgcct
     1501 ataaagatga aaaaacctgt ggtgttctgt ctgaagacga cacttttggt accatcacta
     1561 tcgctgaacc aatcggtatt atttgcggta tcgttccgac cactaacccg acttcaactg
     1621 ctatcttcaa atcgctgatc agcctgaaga cccgtaacgc cattatcttc tccccgcacc
     1681 cgcgtgcaaa agatgccacc aacaaagcgg ctgatatcgt tctgcaggct gctatcgctg
     1741 ccggtgctcc gaaagatctg atcggctgga tcgatcaacc ttctgttgaa ctgtctaacg
     1801 cactgatgca ccacccagac atcaacctga tcctcgcgac tggtggtccg ggcatggtta
     1861 aagccgcata cagctccggt aaaccagcta tcggcgtagg cgcgggtaac actccggttg
     1921 ttatcgatga aactgctgat atcaaacgtg cagttgcatc tgtactgatg tccaaaacct
     1981 tcgacaacgg cgtaatctgt gcttctgaac agtctgttgt tgttgttgac tctgtttatg
     2041 acgcagtacg tgaacgtttc gcaacccacg gcggctatct gttgcagggt aaagagctga
     2101 aagctgttca ggatgttatc ctgaaaaacg gtgcgctgaa cgcggctatc gttggtcagc
     2161 cagcctataa aattgctgaa ctggcaggct tctctgtacc agaaaacacc aagattctga
     2221 tcggtgaagt gaccgttgtt gatgaaagcg aaccgttcgc acatgaaaaa ctgtccccga
     2281 ctctggcaat gtaccgcgct aaagatttcg aagacgcggt agaaaaagca gagaaactgg
     2341 ttgctatggg tggtatcggt catacctctt gcctgtacac tgaccaggat aaccaaccgg
     2401 ctcgcgtttc ttacttcggt cagaaaatga aaacggctcg tatcctgatt aacaccccgg
     2461 cttctcaggg tggtatcggt gacctgtata acttcaaact cgcaccttcc ctgactctgg
     2521 gttgtggttc ctggggtggt aactccatct ctgaaaacgt tggtccgaaa cacctgatca
     2581 acaagaaaac cgttgctaag cgagctgaaa acatgttgtg gcacaaactt ccgaaatcta
     2641 tctacttccg ccgtggctcc ctgccaatcg cgctggatga agtgattact gatggccaca
     2701 aacgtgcgct catcgtgact gaccgcttcc tgttcaacaa tggttatgct gatcagatca
     2761 cttccgtact gaaagcagca ggcgttgaaa ctgaagtctt cttcgaagta gaagcggatc
     2821 cgaccctgag catcgttcgt aaaggtgcag aactggcaaa ctccttcaaa ccagacgtga
     2881 ttatcgcgct gggtggtggt tccccgatgg acgccgcgaa gatcatgtgg gttatgtacg
     2941 aacatccgga aactcacttc gaagagctgg cgctgcgctt tatggatatc cgtaaacgta
     3001 tctacaagtt cccgaaaatg ggcgtgaaag cgaaaatgat tgctgtcacc accacttctg
     3061 gtacaggttc tgaagtcact ccgtttgcgg ttgtaactga cgacgctact ggtcagaaat
     3121 acccgctggc ggactatgcg ctgaccccgg atatggcgat tgtcgacgcc aacctggtta
     3181 tggacatgcc gaagtccctg tgtgctttcg gtggtctgga cgcagtaact cacgccatgg
     3241 aagcttatgt ttctgtactg gcatctgagt tctctgatgg tcaggctctg caggcactga
     3301 aactgctgaa agaatatctg ccagcgtcct accacgaagg gtctaaaaat ccggtagcgc
     3361 gtgaacgtgt tcacagtgca gcgactatcg cgggtatcgc gtttgcgaac gccttcctgg
     3421 gtgtatgtca ctcaatggcg cacaaactgg gttcccagtt ccatattccg cacggtctgg
     3481 caaacgccct gctgatttgt aacgttattc gctacaatgc gaacgacaac ccgaccaagc
     3541 agactgcatt cagccagtat gaccgtccgc aggctcgccg tcgttatgct gaaattgccg
     3601 accacctggg tctgagcgca ccgggcgacc gtactgctgc taagatcgag aaactgctgg
     3661 catggctgga aacgctgaag gctgaactgg gtattccgaa atctatccgt gaagctggcg
     3721 ttcaggaagc agacttcctg gcgaacgtgg ataaactgtc tgaagatgca ttcgatgacc
     3781 agtgcactgg cgctaacccg cgttacccgc tgatctccga gctgaaacag attctgctgg
     3841 atacctacta cggtcgtgat tatgtagaag gcgaaactgc agcgaaaaaa gaagccgctc
     3901 cggctaaagc tgagaaaaaa gcgaaaaaat ccgcttaatc agtagcgctg tctggcaaca
     3961 taaacggccc cttctggggc cgtttttttg ttttcctaaa gcaactattc cataaaccga
     4021 cagcattagc cttcatcata tttgcagcga tgtataacgc ctacacacag cgatattgta
     4081 ctttacaggt cattagtcaa cgtcggtgct taagcgctct gtgaggcgta tagcggcgtt
     4141 aaaaactacc gcgaagggtg tatagcccgg aagaagtgcg taaaacgaac tgacaggata
     4201 aaagtgccct gctcaccctg tcagtaaaga aattcttatt aatcgtggcg atgcctttcc
     4261 tgaatagccg ttaatgagcc gacttgtaac gcctctttat agtgtttacg gcatacagaa
     4321 acgtatcgtt cattaccacc aataaccacc tgctcacctt cgttataagg tctgcctgct
     4381 tgatcaagac gcagcaccat gcttgcttta cggccacaaa aacagatggt ttttaattca
     4441 accagtttgt cggaccatgc cagtaagtat tggctgccaa taaataattc acctcgaaaa
     4501 tcggtacgta aaccataaca aagtacgggt atatcgagtt gatcgacaac ctccgataat
     4561 tcatatactt gttgtctggt taaaaactgg cattcatcaa ccagtacgca atgaattgcc
     4621 tgctgttcat gttccgcacg aatctcatca aataatgatg aattttggtt aaataatttt
     4681 gcaggcgatg acaaacctat acgcgaactg actttcccgg caccaaagcg atcatcaatt
     4741 tctgccgtat atacgacagt gcgcatgccg cgttcctggt aattgtatga agattgcaac
     4801 aatgctgtag acttacccgc attcattgcg gaatagtaaa aatatagctg tgccatcagc
     4861 cacaggccct caatgatatg aaataaagtt ggcaggagtt tatcataatt cgtcagtttt
     4921 tcagctcaac agtatgcaca gaagtgggta tatgcgttct cccttacgaa gccttgcata
     4981 atccttctga gctatcatta caactgcctc gcttgttata agcgggtaaa tgactgctgg
     5041 taactattta caatctttaa cctgttgcgc aagtaatagc cctctgttga cctccaggag
     5101 atagtgcaat actaagtccc tgctcttatt gcgactgttc tacttttcat cattcgctta
     5161 atagggaatt ctcgtaaaca cgactaatac agaagactga aaggtcgtca gcctacgata
     5221 atctccccat aaaatgtgac atgaatcagg aagttttaac ctcacgtgct gcgaaatcat
     5281 cggtgtaaat agggctatat gccgcgtctt ttctggctaa ttttatgaaa agatatttat
     5341 tggcggcaca aaataaagaa caattttgaa ttccttacat tcctggctat tgcacaactg
     5401 aatttaaggc tctattatta cctcaacaaa ccaccccaat ataagtttga gattactaca
     5461 atgagcgaag cacttaaaat tctgaacaac atccgtactc ttcgtgcgca ggcaagagaa
     5521 tgtacacttg aaacgctgga agaaatgctg gaaaaattag aagttgttgt taacgaacgt
     5581 cgcgaagaag aaagcgcggc tgctgctgaa gttgaagagc gcactcgtaa gctgcagcaa
     5641 tatcgcgaaa tgctgatcgc tgacggtatt gacccgaacg aactgctgaa tagccttgct
     5701 gccgttaaat ctggcaccaa agctaaacgt gctcagcgtc cggcaaaata tagctacgtt
     5761 gacgaaaacg gcgaaactaa aacctggact ggccaaggcc gtactccagc tgtaatcaaa
     5821 aaagcaatgg atgagcaagg taaatccctc gacgacttcc tgatcaagca ataatctttt
     5881 gtagattgca cttgcttaaa atcccgccag cggcgggatt ttttattgtc cggtttaaga
     5941 caatttaata agaataagct ataaaaaaac ggcgtcgatt gctcaacgcc gtttcgtgga
     6001 taacaccgat acggatgtta cttcttaatg cccatctctt cttcaagcca ggctttaaat
     6061 tccgtgccaa gggtgttatg acgaataccg tattcaacga aggcctgcat gtaacctaat
     6121 ttattaccgc agtcatggct cttccctttc atatgatagg cttccaccgt ttctttttcg
     6181 atcagcatat caattgcgtc ggtgagctga atttcatcac cagctcccgg aggggttttt
     6241 gccagcaacg gccaaatatc cgcgctaagt acgtaacgac ccacaatagc gagattagac
     6301 ggcgcaacat ccgctttcgg tttttctacc acaccaacca tcggtacgct ttcacccggc
     6361 gctaattcaa cgcctttgca atccacaacg ccatatgcgg tcacatcagc aaccggttca
     6421 accatgatct ggctatgacc cgtttcatca aagcggcgga tcatctctgc caggttatcc
     6481 tgtgacaaat cggattcata ttcatccaga ataacatcag gcaaaataac agctaccggt
     6541 tcatcaccca ctaccgggtg agcacacaat accgcgtgtc ccaggccttt cgccagaccc
     6601 tgacgaactt gcataatagt cacgtgcggt ggacaaatag actgcacttc atcaagcagt
     6661 tgacgtttta cacgtttttc cagcattgct tccagttcaa aactggtatc aaagtggttt
     6721 tcaatagagt ttttagatga gtgtgtaacc agcacaattt cagtaatgcc agccgcaata
     6781 cattcattca cgacgtattg aattaatggc ttatcgacaa gtggcagcat ctctttcggg
     6841 atggctttcg tcgccggcaa catcctggtt cctaatcccg caacggggat aacggctttt
     6901 ttgactttcg tattaatggc agccatttaa attctcctgg actgttcgtg ttttgaacgt
     6961 gttcatattt ctgtatcgca tcccagtata tcagcacccc gaaggggcta agttctgaaa
     7021 aggaggcgag ttagcataat taagacaatt acttatacga atggcgacga tagtaccacc
     7081 agccgacatt agcaggtaat gcaaatttag cccgcgttat cgtttgctca ttctgcagac
     7141 aacatcaagc gcagtcgacc accggttccc catatttggc attgccaggc atcgcatcgc
     7201 tggctcaatt gattcaaata agcgttacct aaagtgccta acggaacacc attactgatt
     7261 tgcacctggt gttcgccggt atttaacgtc gcattcagac ccgcagaaac cagaatgaga
     7321 tttttcagtt cgcgatgata atagccaact aatagcggaa actgccccgg cagattggcc
     7381 tgacgaagta aatggtttac ctgcttcaat aacgcgccca actctggcaa ccgttgattt
     7441 tggtgtgcaa gctgttcctg taataatccg ttaaacaatg cgcgtaataa caaggcagca
     7501 agtacgccat tatgtccagc tcgggtgaca tcaaggcaat aaaatgccag atcgttttcc
     7561 gaaagtgcgg caatatcaag caccaggccg ggtttgtccg cggcaaccaa ttgacgataa
     7621 ttaacccggc aatgggaaat cacctgctga accggcggtt gtagttcctg taataatttc
     7681 gccgctgcgg cagggttatc aaccattgca tcccagtcgc gaaaaagcct ttcctcttcc
     7741 tcaacgcgcg aattaaacat gctgggatag agacaggcaa aaaccatctc gcgcaagcga
     7801 ttcagatctt taactggttt cagcaaaaca tcttcaacgc ccagacgtaa cgctttggca
     7861 atatctgcca tattttcagt ggcagatatc accagaactg gggtctggtc gcctctgtta
     7921 cgtatatgct ccagcagttt aagcccgttc attcgtggca tcgcgatatc acatatcatc
     7981 aggtctggag tgaaacctcc cagcaactca agggcatcca ccccatcagc cgccagtacc
     8041 gttgtcgctc ccaatgagga aaaccacgaa tccagaagcg agcgaaatac ctgctcatct
     8101 tcaacaatga gaatctgttt tccgaccaat ggctgcgtca tgttctctcc cctgactggc
     8161 tttactcaat agtggcatgc tattgccacc tgcgcctgtc agaattaact taagtgtaga
     8221 taaaaattct ggtcaaatgt tggtgcgtac caacggcaaa agttcgtcca ttttcttttc
     8281 cactgccagc tgtctggccg caatggcagc gtgcgcgcga tggaaatcaa gcgtagatat
     8341 ttgcgggcaa acaggttgaa tcagaatatc gggcggatca cctgccatgc ggttcctttt
     8401 aaggcggttc tccagcacct ggatagaagt ggtcataatc tctgttgccg ttggtgctgt
     8461 caccgcccga cgtgtcgtta tgctgcctaa cctttctttc agacgcgcat gccacggcag
     8521 agaatcacca ttctcgctat tttcttcact gacattaaag gagagcaaat cttgttgcat
     8581 caaatgagca tcgtgctgca ggtcgaccgc tatcacaata tcagcaccca atgcacgcgt
     8641 gagcgaaata ggaattgggt taacgactgc cccatcaacc agccagtagc cgttatgcgc
     8701 aacgggtgcc atgagtcctg gaatactgca tgatgcgcga atagcaagat ggagatcgcc
     8761 ttcagtaaac cataattcac gcccagtact taaattggtg gcaacagccg cgaagcgacg
     8821 ggaacaattt tcgatctctg tttccggcat tatttcgcgg tattgattga agacacgctc
     8881 gccgcgcagt aacccaccgc gctgccagga gagatccatc aggcgtaaaa catcccaata
     8941 actgaaagag gttacccaat cttccagcgc agataatcga tcgcatgcat aggccgcgcc
     9001 caccagcgaa ccaattgaac atcctgcaac gatatcaatt tcaataccca ctttttttag
     9061 cgcattaata acgccaatat gcgaccaacc tctcgccgcg ccagatccca gcgccagccc
     9121 tatttttatc tttctcatta ttcccgcaag attcccctgg aatgcaatcg tagccacatc
     9181 gctactgctc ggttaacata gtgctactca ggcggttaat acgccgttat tgtttccagg
     9241 gagattattt gtgtctcagc tttgtccctg tggtagtgct gtcgagtata gcctatgttg
     9301 ccacccttat gtgtctggtg aaaaggttgc acctgatcca gaacatctca tgcgttcgcg
     9361 ttactgcgct tttgtgatgc aagacgcaga ttatttaata aagacctggc atccatcttg
     9421 tggagcagcg gcgttacgtg ccgaattgat tgccggattt gcgcatactg aatggttggg
     9481 attgaccgta tttgaacatt gttggcagga cgccgacaat atcggatttg tcagttttgt
     9541 tgctcgattt accgaaggtg gcaaaacagg tgcaataatt gaacgttcgc gatttttgaa
     9601 agaaaacggt caatggtact atattgacgg tacatgtccg cagtttggcc gcaacgatcc
     9661 ctgcccttgt ggttcaggta aaaaatttaa aaagtgctgc ggccaataat ggttgacggt
     9721 acggtttagc aaacactctc aacaaggttt ttccagcaat gcattcactc caacgtaaag
     9781 ttctgcgtac tatttgtccg gaccaaaaag gtctgatcgc acgtattacc aatatttgct
     9841 acaagcacga gttaaatatc gtacagaaca atgaatttgt tgatcaccgt accgggcgct
     9901 tttttatgcg cacggaactg gaagggattt ttaatgattc caccctgctg gcggatctcg
     9961 atagcgcatt gccagaaggc tccgtgcgtg agctgaatcc tgccggtcgt cgccggatag
    10021 tgattctggt cactaaagaa gcgcattgcc ttggcgattt gttgatgaaa gccaattacg
    10081 gcggcctgga tgtcgaaatc gcggcagtga ttggtaacca cgatacttta cgttctctgg
    10141 ttgagcgttt tgatataccg tttgagctgg taagccatga agggttaagc cgcaacgagc
    10201 acgatcaaaa gatggcggat gccattgatg cttatcaacc tgactacgtg gtgctggcga
    10261 agtatatgcg ggtattaaca ccggaatttg tgtcacgctt cccgaataag atcatcaata
    10321 ttcaccattc cttcctgcca gcgtttatcg gcgcacgtcc ttatcaccag gcctatgaac
    10381 gtggcgtgaa gattattggc gcaaccgctc actatgtgaa tgacaatctg gacgaaggcc
    10441 caatcatcat gcaggacgtt attcatgtcg atcataccta cacagctgaa gatatgatgc
    10501 gcgcaggtcg tgacgtcgag aaaaacgtct taagtcgtgc gctttacaaa gtactggcgc
    10561 agcgcgtctt tgtttacggt aatcggacga ttattcttta atcgccagca aaaataactg
    10621 gttaccttta atccgttacg gatgaaaatt acgcaaccag ttcatttttc tcaacgtaac
    10681 actttacagc ggcgcgtcat ttgatatgat gcgccccgct tcccgataag ggagcaggcc
    10741 agtaaaaagc attaccccgt ggtggggttc ccgagcggcc aaagggagca gactgtaaat
    10801 ctgccgtcat cgacttcgaa ggttcgaatc cttcccccac caccatcact ttcaaaagtc
    10861 cctgaacttc ccaacgaatc cgtaattaaa tattctgccc aagcggggaa ggatgagaag
    10921 cttcgaccaa ggttcgactt gagcgccagc gagagagcgt tgccgctggc aacgacccga
    10981 agggcgaagc gcgcagcgct gagtaatcct tccctcacca ccatcgcttc cttaaataat
    11041 caaaacctgt tactccgagc taattttgcc cgatggcacc acgtttaccg ggcctacaat
    11101 atcctcccct gtaggtcgga taagcgaagc gcatccgaca actcaccccg caccgtgccc
    11161 ataaaaaaac cccgccgaag cagggtctat gtgaactaaa attcgcttag tgacgagcgc
    11221 gcaccaactg atacttacgt gtcagatact ccaccggtac gctccagatg tgcaccagac
    11281 gcgagaacgg gaacagcagg aataacgtca tccccaacac caggtgcaga cggaagataa
    11341 acgctacacc atcaaggtgt tgagaagcgc caccgtggaa ggtcactacc gactgcgccc
    11401 agccaaccag tttcatcatc tcactaccgt ccatatgctg ggcggagaac ggaatggtca
    11461 acaggcccag cgcgcactgg ataacgagca gcgacaggat caggatatcc gctccggtag
    11521 tggttgcacg cacgcgtggg ctgaacagac gacgcttaag caacagcacg ccgccaatca
    11581 gacacagcac gccgctggca ccaccagcaa acatggccat tttctgtttc acttcaatcg
    11641 gcagccaggc ttcatacatc cagtgcggcg tcagcatacc gaagaagtga ccgacaaaaa
    11701 tccccagaat accgatatgg aacaggttcg acgccaggtt catccctttg cgatccagca
    11761 tctggctgga cgccgcgcgc caggtgtact gcccgtagtc ataacgcagc cagctaccaa
    11821 tcaggaagac cgccccggct atgtacgggt agatatcaaa gaagaacata ttcaggaatt
    11881 gcattagtgc tgtcctccgg tggtgatatt cagatattgc ggcgcgacgg caccggcaaa
    11941 gcgacgctga tgagcagtga ttgctgaatc accgcagcct ttgtcagcaa agaatttaac
    12001 ctgctcttct tcccagacag catccagcgc ctgcggcgta tcatcgcgcg cttcgtcggc
    12061 aattttttcc gccactttgt cgctgtcgat agcggtattg gccaatttca gcagcagatc
    12121 aaacagcacg gcataacggc tttcacgctg ttgcagacgc gcgctcagca atgccagaat
    12181 cggcgcgata tctttcaaac cttccacggc ttcgctttgc ggcagctgcg ccaggtactc
    12241 cagatacagc ggcagatggt ccggcaattc gcggctgttt aactgcaagc cgtgctgctc
    12301 gtactgcgcc agcaggtcca ccatcgcctg gccgcggtcg cgggattcgc cgtgcacatg
    12361 ttcaaacagc aacagtgacg tggcgcggcc acggtcgaac agttcgctgt actgcgcctg
    12421 ggcatcgagc ggatccatcg tcgttaaatc gcgcaggaaa atgcccagcg catgggcatc
    12481 ctcttttggc agatttttcg acgcggcaat cgcctcaaac atctcttgtt gatgctgcca
    12541 taaggcagca tccggatatt caaggagacg cgatacaatc acgagttcga tcatgggtgc
    12601 ggctccgttt tgctggttac gtcgatggca tcgatacggc ggctgttgaa cagattgaat
    12661 ttggtatctg aaccgtggca gccatcacca aaggtaaagc cgcagccatt tttctccggg
    12721 aaggcttccc gcgccagttc acgatgacta ctcggcacca caaagcgatc ttcgtagtta
    12781 gcaatcgcca gataacggta catctcctgt gcctgggctt cggtcagacc gacctcttcc
    12841 agcgcacggg tatccacttt cccgtcaacg gtttcagcac gtttataatg acgcatcgcc
    12901 agcatacgtt tcagcgcgcg cagtaccggt ttggtatcac cggcggtcag cagattcgcc
    12961 agatactgta ccggaatacg caggctttcg acgtctggca gaatgccgtt gctacccagc
    13021 tcacccgcgt ctgctgcaga ctgaatcgga gacagaggcg gcacgtacca gaccatcggc
    13081 agtgtgcgat attctggatg cagcggcagc gccagtttcc attccattgc cattttataa
    13141 accggcgact gctgtgcagc ttcaataacg ctcagcggaa taccgtcttt aatcgcctgc
    13201 tcgatgactt tcggatcgtt cgggtcgagg aacacctcca gctgacgctg gtagagatct
    13261 ttctcgttct cggtgctggc tgcacattca atcgcgtcgg catcgtacag cagcacgcca
    13321 agataacgga tacgaccgac acaggtttct gagcacacgg tcggctgacc cgcttcaata
    13381 cgcggatagc agaaaatgca cttctcagac ttaccgctct tccagttgaa gtagattttt
    13441 ttgtacgggc atccggtgat gcacatacgc cagccacggc atttatcctg gtcgatcagg
    13501 acgatgccat cttcttcacg cttgtaaatc gcaccgctcg ggcaggtcgc cacacatgcc
    13561 gggttcaggc agtgttcgca caggcgcggc aaatacatca tgaaggtgtt ttcgaactgg
    13621 ctatacatcg ccttctggat gttgtcgaag ttcttgtctt tcgccagttt gtcaaactca
    13681 ccgcccaggt catcttccca gttcggccct ttttcgattt tcgccatccg ttccccggta
    13741 atcagcgaac gcggacgggc aatcggctgc gatttgctgc cttccggcgc ggtatgcagg
    13801 ttctgatagt caaaatcgaa cggctcgtaa taatcgtcga tccccggcag atgcgggtta
    13861 gcgaagattt tacccagcag catggcacgg ttacccatgc gcggctgcag tttgccgttg
    13921 attttacgga tccagccgcc tttgtatttt tcctggtttt cccagtcagt cgggaagccc
    13981 tggcccggct tggtttccac gttgttgaac cacgcgtatt ccacgccttc acggctggtc
    14041 cagacgtttt tacaggtaac tgaacaggtg tggcagccga tgcacttatc gagattcagc
    14101 accatgccga cttgtgaacg aattttcatt ttacgctctc ctgtacctgg tcattgcctt
    14161 cgccatctaa ccagtcaatg ttcttcatct tacgcactac aacaaactca tcgcggttag
    14221 aacctacggt gccgtagtag ttaaagccgt atgccagatg tgcatagccg ccgatcatat
    14281 gcgtcggttt cggcgtgata cgggtgaccg agttatggat accaccacgc tgttgggtaa
    14341 tttccgaacc aggcaggtta acgatacgtt cctgcgcgtg gtacatcatg gtcatccctg
    14401 ccggaacacg ctggctgaca accgcacggg cagtcagcgc accgttgctg ttgaagactt
    14461 caatccagtc gttatcggcg atacccagat ctttggcatc ggcttcactc aaccagacta
    14521 ccggaccacc gcgacctaaa gtcagcatca gcaggttgtc gctgtaggtg gagtggatac
    14581 cccacttctg gtgcggcgtc aggaagttga gcgctttttc cgggttgccg ttggatttct
    14641 ggcctatcac ttctttcacc gaacgggtgt cgatcggcgg acgataaacc agcaggcttt
    14701 caccgaaatc acgcatccac tggtgatcct gatacagttg ctgacgacca gagagcgtac
    14761 gccatgggat cagctcgtga acgttggtgt aaccggcgtt gtaagaaacg tgttcatctt
    14821 ccagaccaga ccaggtcggg ctggagataa ttttgcgcgg ctgtgcctga atatcgcgga
    14881 agcggatctt ctcgtcttct ttattcagcg ccagatgcgt atggtcacga ccggtaaatt
    14941 cgctcagggc agcccaggct ttcacggcta cctgaccgtt ggtttccggt gccagtgtca
    15001 ggatcatctc tgccgcatca attgcggtgt tcagcatcgg ctggcctttc gccggacctt
    15061 ccgctttggt gtagttgagc ttacgcagca gatccatctc gctctgggtg ttccaggcaa
    15121 tccctttacc gccgttaccg attttctcca tcagcgggcc gatagaagta aagcgttcgt
    15181 aagttgccgg ataatcgcgc tctacgacca taatgtgcgg tgcagttttg cctgggatca
    15241 ggtcgcattc gccttttttc cagtctttca catccagcgg ctgcgccagt tcagcggcag
    15301 agtcgtgctg gataggcagc gtgacgatat cggtttcttt acccagatgg ccgacgcaca
    15361 cttcggagaa tttcttcgcg atggctttgt agatttccca gtcgcttttc gcttcccagg
    15421 ccggatcgac cgccgcagac agcgggtgaa taaacggatg catatccgaa gtattcatgt
    15481 cgtctttttc gtaccaggtc gccgtcggca aaatgatgtc ggaatagagg caggtgctcg
    15541 acagacggaa gtccagcgta accaccagat ccagcttgcc ttccagacca ttgtcctgcc
    15601 agtccacttc ttccggcttc acgccgccct gctgccccag atctttaccc tggataccgt
    15661 gctccgtccc cagcaggtac ttgagcataa actcatgacc tttaccggaa gaaccgagca
    15721 ggttagaacg ccagatgaac aggttgcgcg ggtggttttt accgttttct ggttgttctg
    15781 ccgcaaaacg gatggaaccc tctttcaggg atttcaccgt atagtccacc ggattcatcc
    15841 cggctttttc cgcttcgcca gcgatagtca gcgggttagt gcctaactgc ggtgcagacg
    15901 gcagccagcc catgcgttcc gcacggacgt taaagtcgat caggtgtccg gtataacggg
    15961 atttatccgc catcggtgac agcaactctt ccgccgtgac ggtttcataa cgccactggc
    16021 tggagtggtt atagaaataa gaagtgctgt tcatgtgacg cgccggacgc tgccagtcaa
    16081 gggcaaacgc cagcggctgc cagccggttt gcggacgcag tttttcctga cctacatagt
    16141 gcgcccagcc gcccccgctc tgaccgacac agccgcagaa aatcagcatg ttgatcagac
    16201 cacgatagtt catatcgagg tgataccagt ggttcagccc cgcaccgacg ataatcatcg
    16261 aacgaccgtg cgttttatca gcgttatcgg caaattcacg ggcgatgcga ataatctggc
    16321 tgcgagaaac gccggtaatc tgctcggccc aggccggggt ataagctttc acatcgtcat
    16381 agctggttgc acagttaacg tcgttcaggc cacgttccag accgtagttt gccagcgtca
    16441 gatcataaac ggtggtcacc agggcggtgc tgccatcagc cagttgcagg cgtttcaccg
    16501 gcagtttgtg cagcagcacg ttttccagtt ccactttgtt gaagtgttcc gtgccgtcgc
    16561 caccaaagta cgggaagcct acctcagcga tctcatcctg gctacccagc aggctcagtt
    16621 gcagctcggt ttcttcgcca gttttgccgt cgcgctgctc aagattccat ttgcccttct
    16681 cgccccagcg gaagccaata gaaccgttcg gcgcaaccat ttcgccattg gtattaaagg
    16741 cgacagtttt ccattccgga ttgttttcct ggcccagcgc atcaaccaga tcagcagcgc
    16801 gcagcatacg acctgcagcg tagtaaccgt cgcgttcttc cagcatcacc agcatcggca
    16861 tgtcggtgta gcgacgcaca tagtcggtga aatactggct tgggttgtcg agatggaatt
    16921 cacgcagcat tacgtggccc atcgccagcg ccattgccgc atcggtgccc tgtttcggtg
    16981 ccagccacag atcgcacagt ttggcgattt cagcgtagtc tggtgtgacg gcaacagttt
    17041 tggtgccttt gtaacgcact tcagtaaaga agtgagcatc tggggtacgc gtctgcggca
    17101 cgtttgaacc ccaggcgatg atgtagctgg agttgtacca gtcagcagat tccggtacgt
    17161 cagtttgctc gccccaggtt tgcggcgacg caggaggcag gtcgcagtac cagtcgtaga
    17221 agcttaagca agtaccgcca atcagcgaga gatagcgtgc acccgatgcg taagaaacca
    17281 tcgacattgc cggaattggc gagaaaccag caacacggtc cgggccgtag tttttgatgg
    17341 tgtaaacgtt agatgcggcg atcagttcgt tcacctcctg ccaggaagaa cgaacaaaac
    17401 caccgcgtcc acgcgcctgc ttaaagcttt tcgctttatc ggcgtcttca atgatagaag
    17461 cccatgcctc aaccggatcg ctatgcagcg ccttcgcttc acgccacatt ttcatcaggc
    17521 gtttgcgcat catcgggtat ttcaggcggt tggcactgta aagataccag gagtagctgg
    17581 caccgcgcgg gcagccgcga ggttcatggt ttggcagatc cggacgggta cgcggatagt
    17641 cagtctgctg ggtttcccaa gtgaccagac cgtttttgac gtagattttc cagctgcagg
    17701 agccggtgca gtttaccccg tgggtagagc ggacgatttt gtcatgctgc caacgctggc
    17761 gatatccatc ctcccagtca cggttggtat tgagaagctg gccatgccca tcggcaaagg
    17821 tttcaccctt ctgtttgaag tagcgaaacc ggtccaggaa tttactcatc ggttttctcc
    17881 tgtgggagcc tgtcggccct ctgataaatc gacattgcta atgtttaagg gcaaggtaac
    17941 gctctgaaac agcgtgggaa ttgataacga tcaagagtga tggggatgaa aaataaagta
    18001 aattcctaag ataccccttt aggataatta acagttacag cataacatgc tgtaaattaa
    18061 gacgttttat acctgtagtt atttaaact
//
LOCUS       DAPGOF010000096        18040 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.096, whole genome shotgun sequence.
ACCESSION   DAPGOF010000096 DAPGOF010000000
VERSION     DAPGOF010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18040
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.096"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(182..1774)
                     /gene="lsrK"
                     /locus_tag="RHV13_002871"
     CDS             complement(182..1774)
                     /gene="lsrK"
                     /locus_tag="RHV13_002871"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310145.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HDV1242387.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTIRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKVGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPTSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPAPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(1853..2806)
                     /gene="lsrR"
                     /locus_tag="RHV13_002872"
     CDS             complement(1853..2806)
                     /gene="lsrR"
                     /locus_tag="RHV13_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HDV1242388.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHIRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            3055..4590
                     /gene="lsrA"
                     /locus_tag="RHV13_002873"
     CDS             3055..4590
                     /gene="lsrA"
                     /locus_tag="RHV13_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HDV1242389.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYVRLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAAGTSVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNDKEINRLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNICALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4584..5612
                     /gene="lsrC"
                     /locus_tag="RHV13_002874"
     CDS             4584..5612
                     /gene="lsrC"
                     /locus_tag="RHV13_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HDV1242390.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSVPLLLGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYAHFMTPPPSVKPAS
                     SGKKREAA"
     gene            5612..6604
                     /gene="lsrD"
                     /locus_tag="RHV13_002875"
     CDS             5612..6604
                     /gene="lsrD"
                     /locus_tag="RHV13_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HDV1242391.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6616..7638
                     /gene="lsrB"
                     /locus_tag="RHV13_002876"
     CDS             6616..7638
                     /gene="lsrB"
                     /locus_tag="RHV13_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HDV1242392.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKIAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7665..8540
                     /gene="lsrF"
                     /locus_tag="RHV13_002877"
     CDS             7665..8540
                     /gene="lsrF"
                     /locus_tag="RHV13_002877"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HDV1242393.1"
                     /translation="MADLDDIKDGKDFRTDQPQKNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGNTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            8564..8854
                     /gene="lsrG"
                     /locus_tag="RHV13_002878"
     CDS             8564..8854
                     /gene="lsrG"
                     /locus_tag="RHV13_002878"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HDV1242394.1"
                     /translation="MHVTLVEINVHEDKIDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            8911..9669
                     /gene="tam"
                     /locus_tag="RHV13_002879"
     CDS             8911..9669
                     /gene="tam"
                     /locus_tag="RHV13_002879"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HDV1242395.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYIADLGCGPGN
                     STALLNQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCKVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQHFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(9673..10587)
                     /locus_tag="RHV13_002880"
     CDS             complement(9673..10587)
                     /locus_tag="RHV13_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HDV1242396.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQPVLASNSPANRIL
                     LIMGEWLAVQHRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGIENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(10794..12245)
                     /gene="uxaB"
                     /locus_tag="RHV13_002881"
     CDS             complement(10794..12245)
                     /gene="uxaB"
                     /locus_tag="RHV13_002881"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HDV1242397.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(12472..13890)
                     /locus_tag="RHV13_002882"
     CDS             complement(12472..13890)
                     /locus_tag="RHV13_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDV1242398.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHKCGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(14029..14388)
                     /locus_tag="RHV13_002883"
     CDS             complement(14029..14388)
                     /locus_tag="RHV13_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HDV1242399.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLDKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEQQQR
                     YIVAVIYHWLVIQMNQP"
     gene            complement(14388..15314)
                     /gene="glsB"
                     /locus_tag="RHV13_002884"
     CDS             complement(14388..15314)
                     /gene="glsB"
                     /locus_tag="RHV13_002884"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HDV1242400.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(15378..16766)
                     /gene="sad"
                     /locus_tag="RHV13_002885"
     CDS             complement(15378..16766)
                     /gene="sad"
                     /locus_tag="RHV13_002885"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HDV1242401.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGADDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENTLGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITIAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            16867..17748
                     /locus_tag="RHV13_002886"
     CDS             16867..17748
                     /locus_tag="RHV13_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV1242402.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAIVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSARQGYQ"
     gene            17826..>18040
                     /gene="yneK"
                     /locus_tag="RHV13_002887"
     CDS             17826..>18040
                     /gene="yneK"
                     /locus_tag="RHV13_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HDV1242403.1"
                     /translation="MVTPVSISNYISLPDDFPVHNIAPQVKEVLKDFIDALSTIICNE
                     EWRTSLNINSAIKKIFNNLDNLSYIQRT"
BASE COUNT         4544 a         4584 c         4486 g         4426 t
ORIGIN      
        1 aataagggtt atgcttaatt ctttaagaaa aatagcaatt ttcccttgaa tatagtactg
       61 gtgatgaaac gatgaatctg caagctggca tttaacaagc cagctctaaa agaagggaaa
      121 taagaataac tatactcaaa aactaacagc cacggtcatc atgatgtggc tgtcaatgaa
      181 actataaccc aggcgctttc cataacgacg tcgtcagtcc atgatcaacc agccccagct
      241 gatcctgata aactgcctgc cacttatcgc gtgaatcctg ataaagttca tgcttttccg
      301 gggctggtgt gtgcgtccgt tcccagcgaa ccaggcgttc tccggtttct gccattgatg
      361 aaaaaattcc ggcaccgacg ccagctgcaa tggcacatcc taatgcagtg gcttctttga
      421 ccaccggaat attgacgggt aatcccgaga catcagcgag aatttgactc cataatttcc
      481 cttttgaacc tccgccagca aaaaccagcg aggtgggatg aatattcgag aaatcagcaa
      541 tttgttgcaa attacacgct gatacaatcg ccgcattttc ttccagcgca cggaacaatg
      601 tcgctttgtt acatttatcc gggtcaatgg acaagttaat aaaggaaggc gcagcgtgat
      661 accaggtttt aaagcgcatt ctgtcggaga agatcggcat tacgccccac gacccaggcg
      721 gcacccgact ggccatctct tccagcagcg tataggtgtc gatgcctaaa cgttcggcaa
      781 tcagtttttc ttcggcacag aaagcatcgc ggaaccagcg catggtaagt ccggtaaaaa
      841 agcttataga ttcagcttgt accatgccag gaataacatg aggattaacg cgcacgttca
      901 tttctgggtc tgtcaccggc gcggctaaat ttacaacttg ctgccagaat gtgccgccaa
      961 gaaccgcggt ttgtgccgga cgcacaacgc ctaaccccag gcaaccaagc tgcacgtcgc
     1021 ctcctccaac gaccaccgga gtgcccacct tcagaccgca gagttccgcc gcttgtgaac
     1081 ttaccacgcc cagcaatgtg ccggtttctt tgacaggaga aagaatatcg gcacgcaggc
     1141 cagccatatc cagcaatgca ggtttccagt cacggatggt tagatcaaga agtcccgtgg
     1201 tgccagcgtt agagggatcc accgcaagtt cgccgctgag catataggcc agccagtcgc
     1261 tgatcatggt gatggttgat gcctgacggt aaatatcgga acgatggtgc gccagccaaa
     1321 gtaatctggg gatggcactt aaagccagtg tttgtccggt cgcgcgataa acttcgtttt
     1381 caaaggtatt gttgtgcagt tctttaagtt cgctaacttc acgcgccgct ctggcatcca
     1441 cattggcgca ggcccagatc ggtgttcctt cattgttata taaaacaatg ccttcacgca
     1501 tcgaacatgc cgaaacggca gcgatatact ccggggctat gccggcgttg tgcaacgcct
     1561 ggcgcataca ctcacacgcc agttgccagt ttttgttgag atcaaattcc atagaaccag
     1621 gaacgtccgg tactgccaga tgccgccact ccgcctgtcc cactgctatt tgattgcctt
     1681 ccaggtcgaa tatcacagcc cgaatacttc cggtgcctgc atccagcgcc atcaggtagt
     1741 actttgattc tgaaggggta aagagtcgag ccattagatt atcctcggct tatgcgttct
     1801 gtctgttcct ctatacgttc tccatcattc ccggtaataa ggtcatgcaa atttaactac
     1861 gtaaaatcgc cgctgctgtg tcctgatcgg taaccagtgc gttgatataa ccgcctttca
     1921 ttgcagcggc aattgcttcg gctttatttt ctccccctgc cacgccaacc cggacgggta
     1981 tggtcttcag cgcgcttaaa ggtaagccaa tcagttcgtt atgtattttg atatccgtga
     2041 caacgtcacc ttttgcatca aaaaagtagc ctaaaatgtc gccaaccgcc ccttttcggc
     2101 caatcattaa ctgttcgccc tggctgatat aaccggagcg aatgatcgtc gcgtcgtctt
     2161 gttgactcac ggccccaatg ccgacaatcg ccacatccgc tgcttgcgcg gctaacagaa
     2221 catctttgac gcaattttca ttttttagcg tacgggcaat gtcagcggag gatgcccgca
     2281 acggagccgg aataatattc acactgcacg ccgcattaag ctgtccgata cccgtcatat
     2341 aagaaccgac gccaccggag agcgtgacca ggcgaatttg ctgtgacgaa ataaaaccac
     2401 ttaagcgttg cagcgtattc atggttgcct cgccaaaacc aatcgccagc atctgttgtg
     2461 gttgaagtaa actcatcaac atatgcgccg cgcctatccc tagtcgcccg ccgacatcag
     2521 catccgcaag cccagggatc acccggatat gttgcagcga aaactgacga cgtaattgag
     2581 tttcatattc cagacagcct tcaaagcgag aattaatctg tacgcgaata atgccggact
     2641 gatgcccttt ctccagtaat cgcgacactt tcaaacgtgt caggccgaga cgatcgctga
     2701 tctcactctg ggtcagcccg tcgtggtagt aaaaccacgc gatccgcgcg acctgttctt
     2761 cttcacacat tccctgttct gaaattgccg aatcgttgat tgtcataatt cattcttcac
     2821 tttgaacata tttaaatctt taatgcaatt gttcagttct tgcgcattta tatctgtgat
     2881 ggcaaccaca gtttgactct acgagtatga acaaacgcaa ccgtaaaaat caaaatagca
     2941 taaattgtga tctattcgtc ggaaatatgt gcaatgtcca cctaaggtta tgaacaaatt
     3001 aaaagcagaa atacatttgt tcaaaactca cctgcaaaac tgaacggggg aaatatgcaa
     3061 acgagtgata cccgcgcgtt accgctactt tgcgcccgct cggtttataa acagtattca
     3121 ggggttaatg tcctgaaagg catcgatttt acgttgcacc agggggaggt ccacgccctg
     3181 ctcggcggca atggtgccgg taaatcgacg ttaatgaaga ttattgccgg tattacccct
     3241 gctgatagcg gtacgctgga gattgggggc aacaactacg tcagattaac gccagttcat
     3301 gctcatcagc tgggtattta tctcgttccc caggaaccgc tgcttttccc aagcctgtcg
     3361 ataaaagaaa acatcctgtt tgggctggca aaaaaacagc tctccatgca gaaaatgaag
     3421 aacttgctgg cggcgctggg ctgccagttt gatctgcata gtctggcagg atcgctggat
     3481 gtcgccgatc gccaaatggt ggaaatcctc cgcgggctga tgcgcgactc gcggattctg
     3541 atcctcgatg aacctaccgc ctcgcttacc cctgcggaaa ccgaacgctt gtttagtcgc
     3601 ttgcaagagc tgcttgctac tggcgtgggt attgttttta tctcgcataa gctgccggaa
     3661 attcgccaga ttgccgatcg aattagcgtg atgcgcgacg gaaccatcgc cttaagcggc
     3721 aaaaccagcg aactgtctac cgacgacatt attcaggcca tcaccccagc ggtacgggaa
     3781 aaatcgctct ctgccagcca aaaattatgg ctggagttac ctggcaaccg cccacaacat
     3841 gccgcaggaa cgtcggtgct gacactggaa aatctgactg gcgaaggttt caggaatgtc
     3901 agcctgacgc taaatgccgg agaaattctg ggtctggctg ggctggtggg tgccggacgc
     3961 acagaactgg ccgagacgct ctatggtcta cgtactttgc gtggcggacg cattatgctg
     4021 aatgataaag agatcaatag attatccact ggagaacgtt tactgcgcgg tctcgtttat
     4081 ctgccggaag atcgccagtc atccggactg aatctcgatg cttcgctggc ctggaacatc
     4141 tgcgccctta ctcataacct tcgtggattc tgggcgaaaa ccgcgaaaga taatgccacc
     4201 ctggaacgtt atcgtcgggc gctgaatatt aaatttaacc aaccggaaca agctgcacgg
     4261 acattatccg gtggcaacca gcaaaaaatc ctcattgcca aatgcctgga agcttcgccg
     4321 caagtattga ttgtcgatga gccgacgcgc ggcgtggatg tctcggcgcg taatgatatc
     4381 taccaactgt tgcgcagcat cgccgcacag aatgtggctg tgctgcttat ctcctctgat
     4441 ctggaagaga tcgaactgat ggcagatcgt gtgtatgtga tgcatcaggg cgaaattacc
     4501 cactctgcac tgaccgggcg cgatattaat gtcgagacta ttatgcgcgt tgccttcggc
     4561 gatagtcagc gtcaggaggc gtcatgctga agtttattca gaacaaccgt gaaatcacgg
     4621 cactgctggc ggtggtgctg ctgtttgtat tacccggttt tctcgaccgc cagtatttaa
     4681 gtgtgcaaac gctgaccatg gtttatagca gcgcgcaaat cctgatcctg ctggcaatgg
     4741 gcgcgacgct ggtaatgctt acgcgcaata ttgatgtttc agtgggttcg attaccggaa
     4801 tgtgcgcggt gctgttgggg atgttactga acgcggggta ttcactacct gttgcttgtg
     4861 tcgcgacttt actgcttggt ttgctcgcgg gatttttcaa cggtgtcctg gtcgcgtggc
     4921 taaaaatccc tgccattgtt gccacccttg gcacgttagg gttgtacagg ggcatcatgt
     4981 tgctgtggac tggcggcaaa tggattgaag ggttacccgc cgaactgaaa cagctctccg
     5041 tcccgctgct gcttggcgtt tcagcaattg gttggttgac gataattctg gtggctttta
     5101 tggcctggct gctggcgaag acggcgtttg gacgcagttt ttatgccacg ggcgataatt
     5161 tacagggcgc tcgtcaactg ggcgttcgta cggaagccat tcgcattgtg gcattttcgt
     5221 tgaacggctg catggcggca ctggcgggaa ttgtgtttgc ttcgcagatt ggttttatcc
     5281 ccaaccagac cggtaccggg ctggagatga aagcaattgc agcctgcgtg ctgggcggca
     5341 ttagtttgct cggcggttcc ggtgcgatca tcggtgcggt actcggcgca tggttcctga
     5401 cgcagatcga tagtgtactg gtgctgttgc gcattccggc atggtggaac gattttatcg
     5461 cgggcctcgt gctgctggcg gtgctggtgt ttgatggacg cctgcgttgc gcgctggaac
     5521 gtaatctgcg gcggcaaaaa tatgcccact ttatgacgcc accgccatcc gttaaacccg
     5581 cttcgtcagg taaaaaacgg gaggccgcat aatgcgtatt cgctacggtt gggaactggc
     5641 tcttgccgca ctgctcgtta ttgagattgt cgcatttggt gcaattaacc cgcggatgtt
     5701 agatctcaat atgttgctgt tcagcaccag tgactttatc tgcattggca ttgtcgccct
     5761 accgctgacg atggtgattg tcagtggcgg gatcgatatt tcgtttggtt cgaccatcgg
     5821 cctctgcgcc attgcattgg gcgtactgtt tcaaagtggt gtgccgatgc cgctggcgat
     5881 tctcctgacc ttactgctcg gcgcattgtg cgggctgatc aacgccggat taattatcta
     5941 taccaaagtt aacccgctgg tgattacgct tggcacgctg tatctgtttg ccggaagcgc
     6001 cctactgctt tccgggatgg ccggagcgac ggggtacgaa ggtattggtg gattcccgat
     6061 ggcgtttaca gatttcgcta acctggatgt gctgggactc cccgttccgc tgattatctt
     6121 cctgatatgt ctcctcgttt tctggctctg gctgcataaa acccatgccg gacgtaacgt
     6181 gtttttgatt gggcaaagcc cgcgcgtggc actttatagt gcgattccag ttaaccgcac
     6241 cttatgtgcg ctctatgcca tgacggggct ggcgtctgcg gtcgccgctg tgctgctggt
     6301 gtcgtatttt ggttcagcac gttccgatct cggtgcgtcg tttctgatgc ccgccatcac
     6361 cgccgtggtg cttggcgggg ccaatattta tggtggttcc ggttccatta tcggcaccgc
     6421 cattgcggtt ttattagtgg gatatttgca acaaggtttg caaatggcag gagtgccaaa
     6481 tcaggtgtcc agcgcccttt ccggtgcgct acttatcgtc gttgtcgtag gtcgttccgt
     6541 tagcctgcat cgccagcaaa ttaaagagtg gctggcgcgt cgggccaata acccattgcc
     6601 ataaaggata tcttcatgac acttcatcgc tttaagaaaa tcgccttact tagcgctctt
     6661 ggcattgccg caatctctat gaatgtgcag gccgcagagc gtattgcatt tattcccaaa
     6721 ctggttggcg tgggattttt taccagcggt ggcaacggcg cacaacaagc gggtaaagag
     6781 ctgggcgttg atgtgaccta cgacgggccg acagaaccca gtgtttctgg tcaggtacag
     6841 ttgattaata acttcgtcaa tcagggttat aacgccatta tcgtttctgc ggtttcgcct
     6901 gatggcttgt gtccggcact gaaacgcgcc atgcaacgtg gtgtgagagt gctgacctgg
     6961 gactctgata ctaaaccgga gtgccgctct tactacatta atcagggaac gcccgcccag
     7021 ttgggaggta tgttggtgga tatggcagcg cgtcaggtga ataaagacaa agccaaaatc
     7081 gcattttttt attccagccc caccgttacg gaccaaaacc agtgggtgaa agaagcgaaa
     7141 gcgaaaatcg ccaaagagca tcctggctgg gaaattgtca ctacgcagtt tggctataac
     7201 gatgccacta aatcgttaca aaccgcagaa ggaatattaa aagcgtatag cgatctcgac
     7261 gccattatcg cccccgatgc caacgccctg cccgctgccg cacaagccgc agaaaacctg
     7321 aaaaatgaca aagttgcgat tgtcggattc agtacgccaa acgtgatgcg tccatatgtg
     7381 gaacgcggca cggtgaaaga atttggcctg tgggatgtgg ttcagcaagg caaaatttca
     7441 gtgtatgtcg cggatgcatt attgaaaaaa ggatcaatga aaacgggcga caagctggat
     7501 atccagggcg taggtcaggt ggaagtctcg ccaaacagcg ttcagggcta tgactacgaa
     7561 gcggatggta atggcatcgt actgttaccg gagcgcgtga tattcaacaa agagaatatc
     7621 ggcaaatacg atttctgatg tgcattactt aaccggagta agttatggca gatttagacg
     7681 atattaaaga tggtaaagat tttcgtaccg atcaaccgca aaaaaatatc ccttttaccc
     7741 tgaaaggttg cggtgcgctg gattggggaa tgcagtcacg cttatcgcgg atatttaatc
     7801 cgaaaacggg taataccgtg atgctggcct ttgatcatgg ttattttcag gggccgacta
     7861 ccggacttga acgcattgat ataaatatcg ccccgctgtt tgaacatgcc gatgtattaa
     7921 tgtgtacgcg cggcattttg cgcagcgtag ttccaccggc gaccaataag ccagtggtac
     7981 tgcgggcgtc aggtgcgaac tctattctgg cggaattaag taatgaagcc gtggcgttat
     8041 cgatggatga tgccgtgcgc ctgaacagtt gcgcggtggc ggcgcaggtt tatatcggca
     8101 gcgaatatga acatcagtcg atcaaaaata ttattcagct ggttgatgcc ggaatgaaag
     8161 tgggaatgcc gaccatggcc gtgactggcg tgggcaaaga tatggtgcgc gatcagcgtt
     8221 atttctcgct cgcgactcga atcgccgctg aaatgggggc gcaaattatc aaaacctatt
     8281 atgtcgaaaa aggttttgaa cggattgttg ccggatgtcc ggtacccatt gttattgctg
     8341 gcggtaaaaa attaccggag cgcgaggcgc tggaaatgtg ctggcaggct atcgatcagg
     8401 gcgcttctgg tgtggatatg gggcgtaata ttttccagtc tgaccatccg gtggcgatga
     8461 tgaaagccgt acaggcggtg gttcaccata acgaaacggc tgatcgggca tatgaactct
     8521 atctgagtga aaaacagtaa ctgcggattt aaggagaaga attatgcacg tcacactggt
     8581 tgaaattaac gttcatgaag acaagattga cgagtttatc gaagtttttc gccagaacca
     8641 cctgggctct gtacaggaag aaggcaattt gcgcttcgat gtcttacagg acccggaagt
     8701 gaattcgcgc ttttatatct acgaagccta taaagatgaa gacgcagtgg cattccataa
     8761 aaccacgccc cactacaaaa cctgtgtcgc gaaactggaa tctttaatga ccgggccgcg
     8821 taaaaaacgt ctgttcaatg ggttgatgcc gtgaggcgaa ttcatcagtt ttatctacaa
     8881 ttggggtaac gcgctgacgg gagtaaaaaa atgtctgact ggaacccctc tttatatttg
     8941 cacttttccg ctgaacgatc gcgtccggcg gtggagctgc ttgccagagt gccgctggaa
     9001 aatgtcgaat atattgccga tcttggctgt ggcccaggta acagcaccgc ccttctaaac
     9061 caacgttggc ctgcggccag gataacaggc atcgactcgt ctccggcgat gattgctgaa
     9121 gcgcgcagtg ctttgccaga ctgccagttc gtggaagcag acatccgcaa ctggcaaccg
     9181 gaacaggcac tcgatctgat ttttgctaat gcctcactgc aatggctgcc cgaccactac
     9241 gaattgtttc ctcatctggt ttcgttactt aatccgcagg gcgtgctggc agtacagatg
     9301 ccagataact ggctggagcc gacccatgtg ctcatgcgcg aagttgcctg ggaacaaaac
     9361 tacccggatc gcgggcgtga gccgctggca ggcgttcatg cttactacga tattttgagc
     9421 gaagccggat gtaaggtcga tatctggcga accacttact atcatcagat gccgtcgcac
     9481 caggcgatta tcgattgggt gaccgccact ggattacgtc cgtggttaca ggatctgacc
     9541 gagagcgaac agcagcattt tcttacgcgc taccatcaga tgttggaaga gcagtatcca
     9601 ctgcaagaga acggacagat actgctggca tttccgcgtc tgtttattgt tgcccggcgt
     9661 acggagtaag tatcacgtca gctggtaatg acgatcggga agaatttttg ctggaatttc
     9721 ggcttcatcg ttcatctgta acaggtcaat ttcaatagcg ttgcagatgg catccagtgg
     9781 taaatcattg ttttcaatac cgaacggatc ttccagttct tccgccagac aatccagcga
     9841 aataaaagtg taggaaatca gcacagagat aaaaggcgtc atgtaatgca ggtccacgac
     9901 cagcgcgaac ggcagcatga tacaaaacag ataaacggta cgatgcaaaa tcagggtgta
     9961 ggcaaaggga attggcgtgt aggcaatgcg ctcgcagcct gccagaaccg ctgaaatatc
    10021 gttaagccga tcgttgaggc taataaacag gatatctgaa agctgcccat tgcggtgctg
    10081 aaccgccaac cactccccca ttattaacaa gatacggtta gctggagagt tcgaagccag
    10141 tacaggctga agatcttcag tcttgagata atgagccagc acttccgcct gtggctgttt
    10201 gcgtaatgtc atgcgtaaac agtgggcgaa ggcgatttgc agccgggcaa actcccttac
    10261 acttgccgaa tccggcaatg tcgtttttac ctcgcgcagt aacgaccgtg aggcaatcat
    10321 caactgcccc caaagttttc gcgcttcaac gtaacgggcg tacccggcat tattacgaaa
    10381 accaagaaaa atggcgatgg cgacaccgag aatgctgaac ggtgcgaggg tgaatttgat
    10441 gcccagatgc gtgtaccagg gcagcatgaa aataacagcg atagaaaaga gaaaattgag
    10501 tagtaagcgc gatgatatct tggataatac tgagccgtgc cagacaaaaa tacggcgcag
    10561 ccagtgttgt tgtggacgaa caatcatggt tatcttcagg cgtgaaaaag tcgccctatt
    10621 aaacgtgatt atgatcacat tctcaagacg ctacttacaa attacctaca agcttacaac
    10681 attaacaggc gctgcatgtg gcacctgtta atgataaagg cacataggat gttgtaacta
    10741 atatagtgaa ataagaaacc cggtcgaaac cgggttcaga agtaacggtg ttattagcac
    10801 aacggacgta cagcttcgcg catccctttt tcaagaatcg catccaggtc attagcaacc
    10861 tgctcgacca gaccaggcac ttgcgtcagg tcctgctccc agtggtcttt ttctgccaac
    10921 acaatcgcta ccagttcctg ggtaccgata acacggtcac gatgctggct ccagagttgc
    10981 tgataacgtt ccagccagtg tgcatcgtcc tgtaccggat atgtttctcc gttacgctca
    11041 ccacgataga acgcaatcaa tgccgctaat gcgaaagtaa ggcgcgccgg aagtgtgccg
    11101 tttgccttct gccctgccag cagctgcggc aggatgcggg tgcggaactt ggtcataccg
    11161 ttgagcgcga tagacagcag ctgatgctta atgtacgggt tacggaaacg cccggtgact
    11221 gcactggcga aagattccag ttcatcacga ggcaaatcca gtaccggaat aatttcttcg
    11281 tagatggctt tttcaacgaa tgcgcaaatt tcagcatcgt tcatcgcttc acctacggta
    11341 tccagccctg cctggaacgc caccggcacc agcgcggtgt gcgcaccgtt gaggattgcc
    11401 actttgcgtt ctttatacgg tttaatatcg tcaacaatca gcacgttgag cggatatttg
    11461 tccagacgca gttcagtcgc taaggatttc ggtccctgaa tcacaaacag gtaaaagtgt
    11521 tcagcggtgt cgaggaaacc atcgtgataa cccagttctt cttccagttt agccacttca
    11581 tcacgcggat agccggtaac gatacggtcc accagcgtag aacagaagct gttagcctga
    11641 tccagccatt gaataaatgc ttctggcagt gcccactctt gcgcatagcg cagcaccagt
    11701 tcacgcaacg cgtcgccatt gtagtcaatc aactcacacg ggatgatgat ccaaccttta
    11761 tccagcgcac cgttgaaatg gctaaaacgt tcgaacagca gacgggtcag ttttgcggga
    11821 tagcttaccg ctggcgcgtc atcgaattta tcgcccgcat ggtagctgat acccgcttcg
    11881 gtggtgttag agaaaacaaa gcgcatttcc ggattgtgcg ccagtttcag gaattcatcg
    11941 tattcactgt agacgctgat ttcacgatta acagagcgaa tcagacgcgc gtcgctgacc
    12001 gcttccccct tctcattcag gccacggata atggtggtgt acagaccatc ctgcgtgctc
    12061 agtgacggcg ggaatgaagt ttcaatcgga cgaacaacga ccacaccaga attcagatcg
    12121 gtgtgctcat tcaggagatc tatttgccag tcaacaaagg cgcgcaggaa gttaccttca
    12181 ccaaactgaa tgatacgttc tggatactgt gcaccgggaa aatcgcgacg atttagtgtt
    12241 ttcacaatgg gttcccttct gattagtcat acaacctgtt ttaattggta cgacaggtta
    12301 gcaaacttta atacgacgaa cccctgtttt gatcaactcc tgatgattaa tgagcagttt
    12361 tatgagaaaa gtgtggcgcg gatcatggtt taatcgagga aaaacgcctt ttcctggatc
    12421 ataaagtggt agaacacatt gcatttaaat cgctctgaat gaacaaagat gtcagacaac
    12481 ttcctcaccg taacgcatag tgctggtacg gttgcgccca tctttcttcg agcgatacag
    12541 acacgtatcg gcttcgacca tcaatttatt aaagtcatcg gtcagcgttc ggtacgatgc
    12601 gcgaccacta ccgacgccaa tacttaccgt gagataaagc gttttttgtt gccaggtgaa
    12661 tggttgcagt tcaacgcctt tacggatttt ttccgccatt agcagaccat cgacaggatt
    12721 caccgacggc actgcaacag caaattcttc gccgcccatg cgcgccacca gccccttatc
    12781 accgacaatc ttctgaatat gccgggcaaa cacgcttaac actttatcgc cacatttatg
    12841 tccatagtta tcgttaatac ttttgaaata gtcgatatca agcagcatga cagtcagatg
    12901 ttgtgtctgt ttcagcgatg gacttttcag cgcctcataa agaccggacc gtgagtaaac
    12961 ctgagtcaga aaatcaaagt cggctcgcag cgcaacttgc ttcattagcg aattgatcgc
    13021 tgccacgcta aaagaaacca taattgggca tatcgccatc gtggcaatac cgagacgtgc
    13081 ggagaacatt tccggaatgg agaacggcga accgaccgag atatcaatca ccgaattagc
    13141 caccagtacg atttccaccg caccggtaac aaaggtcaac agacatgtta cctgcggcgt
    13201 atagcgcact gcacaccaga ttaatgcagg cagcgggaac gccagactcc ccgctccgcc
    13261 aatgactacc gaggcaataa ccgacacaat aagcgcgatg gcgggcatca tctgttctgc
    13321 tttaaagcgc ggcagcactc caggaattgc caacgtcagc atacaaggca cgatcaacac
    13381 gcccgttgag aattgctcac tgaaccagtc ggcaagcaag ggccagaaat ccagactatc
    13441 aatactgacc gaaccaatcg ccccgacaat agcgcataat aatgcggcaa tcagacagta
    13501 attaaatagc cgtaaggcac ttactggctc atacttattt ttccccagac gcttatcccg
    13561 cacgaccagt aaggcgacgg taacaataaa catcatattg gagaaattga tagccagtga
    13621 aaccagtccc cattcggtgg ttatggcatc ataaaccagc atcgccacat aactgatcgc
    13681 atagtaatgc agtcgattaa gccagacata gcgggcaaac acccccgcca ttacgccatt
    13741 cagcggccag aaaagagaaa gttcgtgaac cagccgtaac tctgcaccaa taaaatagaa
    13801 cagcgtggtt agcgcaaaaa tagcgatgct attacgtagc agatgacctt cctggaacaa
    13861 acggaaagtt gaaatggggt gtacatgcat taattatctg tccatacaat tgcgggtggc
    13921 aattatctca acgtaataag taaattctca taaaggatag catgttcgct gacagcggcg
    13981 gcaaataaag gtaccctggg aatgctattt tatcgttttc cgcaattatc atggctgatt
    14041 catctgtata accagccagt gatagattac cgccacgata taacgttgtt gttgctcgct
    14101 taacgaaaca ccctgcggaa tgttatgcca cttagccaga caaccgcgac aacaggttgc
    14161 agtggcgtgc tgagcgataa acaccggatg cccgcgcatg ggggtttgct taccgtcatt
    14221 agccggtaat gccggagcaa gacgtttagc gacaaaatcc gccgcatgtt gttcgatgac
    14281 tggcgcacct ttatccagac aatactgacg ctctttcatg cccaaacgaa agcgagatcg
    14341 aaattttgaa cgggataaac gcgcgaagag tggatcgaga gactgcatta ataaaccgaa
    14401 cgccctaact gtttcgtcaa ttgttcaagg acggcaatgc ccgcaagcga gttaccagca
    14461 tcatccagtt ccggactcca gactgcgatg gccatttcat gcggaacaat cgccacaata
    14521 ccgccaccaa cgccagattt cgccggtagt cccacccgcc aggcaaactc ccccgcgttc
    14581 tggtacatac cactggtcgc catcagcgcg ttaatttgcc gtgcctgcat tggcgtcacc
    14641 acaggttcat caatatgaat agctttcccc tgattagcca gaaagacaaa cgtccgggcc
    14701 agctctacac agctcatttt cagagcgcag taatgaaagt agttttgcag aacggttgtc
    14761 acgtcatgat ggaaattgcc aaacgacttc atcagccagg cgatagccgc atttcgcgcg
    14821 gaatgttcaa attcggaacg cgctaccacc gtatcgtagg aaatatcaga cacaccgctt
    14881 aagccgcgca cgacttccag catacgttgc cgtggtgcgc ttaatcgccc ttgcaacata
    14941 tcgcaaacca ccagcgcacc ggcattaatg aacggattac gcggtatacc ctgctccatt
    15001 tccagttgca ctaaagaatt gaacggtgat ccagacggat ctttgccgac gcgttgccag
    15061 atttcctctt cggagtaatg acgcatggcg acaacgagac tcagcacttt ggaaatagac
    15121 tgaatggaaa aacgttcttg cgcgtctccg gcctgaaaaa gctgtccgtc aacggtacag
    15181 atagcaatgc ccaatcggga accgtctact gtagccagcg ccggaatata atccgcgact
    15241 ttaccctgac caatgagcgg ccgcacttgc cgcaagatgt tttctaaaat tgcattatcc
    15301 atggcgactg ccactttcta ctcctggacc gcaggtctga aaagacctgc gagtatatca
    15361 gagctgaata tgtcgcgtca gatccggtct ttccacaccg tctggatatt acagaattcg
    15421 tgtaagccga aatgggaaag ctcacgacca aagccactct ttttcacgcc accaaaggcc
    15481 actcgcgcgt cgctggcaca ataaccattg ataaacaccc caccgcattc cagacgtgcc
    15541 gccatctgtc tggcctgtgt ttcgtcggtg gtaaaaatgg tcgctgaaag gccgaactca
    15601 ctatcattag ccagttccag cgcatgttct gcatctttcg caatggtgat tgccgcaacg
    15661 gggccaaaca tttcttcccg aaacgcggtc atttctgggg taacattcgc cagaaccgtt
    15721 ggcggatagt agttacctgc cccagccatc ttttccccgc ccagtaacaa acgcgcaccc
    15781 tgcgccaggg ttttctccac ctgatgatgc agctcatcac gtaaatcaaa acgagccatt
    15841 ggtccgagag tgttctcttc gtcacgggga tcgcccattt tcaaggctgc cgcagctgcc
    15901 acaaaacgtt cggtaaatgc agaagcaatt ccctcttcga taataaagcg ttttgccgct
    15961 gcacatacct gtccggtatt ctgataacgt ccggctaccg ccgctttaac cgccagttcc
    16021 agatcggcat cgttaagcac aataaatgga tccgaaccgc ccagttccag tacgcatttt
    16081 ttcagtgccg ctccagcctg tgcgccaata gccgctcccg cacgaacact tccggtcacc
    16141 gtgacagcag caatgcgcga atctttaatc atttggctga caccgtcgtt gtcggcattc
    16201 agccagccat atacgccttg tgggattccc gcatctttaa acacctgggc aatgagctga
    16261 gcacagccca tcacattcgg cgcatgttta agtaagtaac cgttacctgc aagaatgatg
    16321 ggaacagcgc cacgcatcac ctgccataac ggaaaattcc acggcataat cgccagaatc
    16381 gtccccaacg gtcgatactc aataaccgcc tgctgatttt ccaccagcgt aggttccgcc
    16441 ttcagcattg ccggaccatg ttctgcatac cagtcacaca aattcgccga tttcgccact
    16501 tcagcgcgcg cctggttgat tggtttgccc atttcacggg tgatcatttg cgccatttct
    16561 tcgctacggg cgcgcagagc cttaccgata tcacgcagtt tttcagcacg ataatctata
    16621 tttgtctcgc gccagtcgcg aaagcctgct gccgccagct gaagtgcgtt ttcgatatca
    16681 tcagcaccag cccacggcag cacagaaagt tgttcacccg tggcaggatt tatcgaaatt
    16741 gcatgagttg ccggagtaat ggtcatcggg gtatctcctt tatgagttat ggtaaaaaga
    16801 tacgcagatt tactcttgct ttaaaatgaa taatattaag ccacttattc acgaatcgag
    16861 aatattatgg atctgaccca actggagatg ttcaacgcgg ttgccgaagc tggcagcatt
    16921 acgcaggctg cagcaatagt gcatcgcgtg ccgtcgaatc tcactacccg tctacgccag
    16981 ctggaaacag aactgggggt agatctgttt attcgcgaga atcagcgttt acgtctatct
    17041 cctgccgggc ataacttttt acgctatagc caacaaattc tcgcgttagt ggatgaagcg
    17101 cggagcgttg tcgctggcga tgagccgcaa ggtttatttt ctctgggttc gctggaaagc
    17161 accgcagcag ttcgcattcc ggccacgctg gcggaattca accgtcgtta tcccaaaatt
    17221 cagttttcac tttccaccgg cccttccggc actatgctgg aaggtgtgct ggagggaaaa
    17281 cttaatgcgg cgtttattga tggacccatt aaccatactg ccattgacgg gataccggta
    17341 taccgcgagg agctgatgat cgtcacgcca caaggtcatg cgccagtaat ccgtgccagt
    17401 caggttaatg gcagtaacat ttatgccttc cgcgccaatt gttcgtatcg tcgccacttc
    17461 gagagctggt ttcatgctga cggtgccgct ccgggaacta tccatgaaat ggagtcttat
    17521 cacggaatgt tggcctgtgt agtcgcagga gcaggcattg cgcttattcc gcgctctatg
    17581 ctggaaagta tgccggggca tcaccaggtt gaagcgtggc cgttagctga gcaatggcgt
    17641 tggttaacaa cctggctggt ctggcgtcgt ggtgcgaaaa cacgtccgct cgaggcattt
    17701 attcaactgc tggatgtgcc tgactcggca agacagggat atcaatgagc tatttttgat
    17761 agttcttgcg gctaatatgc tctatatagt gatgttccga tgacttatga ctatatgggg
    17821 caaatatggt tacgccagta agcatcagca attacatatc tcttcctgat gattttcccg
    17881 ttcataatat tgcaccgcag gtaaaagaag ttttaaagga ttttattgat gcacttagta
    17941 caataatatg taatgaagag tggcgcacat ctttaaacat caactcagcc ataaaaaaga
    18001 tatttaataa ccttgacaac ttatcatata ttcagaggac
//
LOCUS       DAPGOF010000097        17624 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.097, whole genome shotgun sequence.
ACCESSION   DAPGOF010000097 DAPGOF010000000
VERSION     DAPGOF010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.097"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(304..1332)
                     /gene="epmB"
                     /locus_tag="RHV13_002888"
     CDS             complement(304..1332)
                     /gene="epmB"
                     /locus_tag="RHV13_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HDV1242404.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVITSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRAGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            1374..1940
                     /gene="efp"
                     /locus_tag="RHV13_002889"
     CDS             1374..1940
                     /gene="efp"
                     /locus_tag="RHV13_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDV1242405.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            1992..2117
                     /gene="ecnA"
                     /locus_tag="RHV13_002890"
     CDS             1992..2117
                     /gene="ecnA"
                     /locus_tag="RHV13_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HDV1242406.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            2228..2374
                     /gene="ecnB"
                     /locus_tag="RHV13_002891"
     CDS             2228..2374
                     /gene="ecnB"
                     /locus_tag="RHV13_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HDV1242407.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            2549..2866
                     /gene="sugE"
                     /locus_tag="RHV13_002892"
     CDS             2549..2866
                     /gene="sugE"
                     /locus_tag="RHV13_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDV1242408.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2863..3396)
                     /gene="blc"
                     /locus_tag="RHV13_002893"
     CDS             complement(2863..3396)
                     /gene="blc"
                     /locus_tag="RHV13_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HDV1242409.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(3485..4618)
                     /locus_tag="RHV13_002894"
     CDS             complement(3485..4618)
                     /locus_tag="RHV13_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HDV1242410.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLHFYQNWQPVWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMHTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQINLKPLDINEKTLQQGIQVAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            complement(4681..5040)
                     /gene="frdD"
                     /locus_tag="RHV13_002895"
     CDS             complement(4681..5040)
                     /gene="frdD"
                     /locus_tag="RHV13_002895"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HDV1242411.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            complement(5051..5446)
                     /gene="frdC"
                     /locus_tag="RHV13_002896"
     CDS             complement(5051..5446)
                     /gene="frdC"
                     /locus_tag="RHV13_002896"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HDV1242412.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(5457..6191)
                     /gene="frdB"
                     /locus_tag="RHV13_002897"
     CDS             complement(5457..6191)
                     /gene="frdB"
                     /locus_tag="RHV13_002897"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HDV1242413.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(6184..7992)
                     /gene="frdA"
                     /locus_tag="RHV13_002898"
     CDS             complement(6184..7992)
                     /gene="frdA"
                     /locus_tag="RHV13_002898"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HDV1242414.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            8317..9294
                     /gene="epmA"
                     /locus_tag="RHV13_002899"
     CDS             8317..9294
                     /gene="epmA"
                     /locus_tag="RHV13_002899"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HDV1242415.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEAYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9513..11015
                     /gene="yjeM"
                     /locus_tag="RHV13_002900"
     CDS             9513..11015
                     /gene="yjeM"
                     /locus_tag="RHV13_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HDV1242416.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(11166..14489)
                     /gene="mscM"
                     /locus_tag="RHV13_002901"
     CDS             complement(11166..14489)
                     /gene="mscM"
                     /locus_tag="RHV13_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HDV1242417.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQISQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEIIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(14511..15479)
                     /gene="psd"
                     /locus_tag="RHV13_002902"
     CDS             complement(14511..15479)
                     /gene="psd"
                     /locus_tag="RHV13_002902"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HDV1242418.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(15576..16628)
                     /gene="rsgA"
                     /locus_tag="RHV13_002903"
     CDS             complement(15576..16628)
                     /gene="rsgA"
                     /locus_tag="RHV13_002903"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HDV1242419.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMEFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            16723..17268
                     /gene="orn"
                     /locus_tag="RHV13_002904"
     CDS             16723..17268
                     /gene="orn"
                     /locus_tag="RHV13_002904"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HDV1242420.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            17479..17554
                     /locus_tag="RHV13_002905"
     tRNA            17479..17554
                     /locus_tag="RHV13_002905"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17512..17514,aa:Gly,seq:gcc)
BASE COUNT         4071 a         4541 c         4625 g         4387 t
ORIGIN      
        1 cataagtagc cgtgctcact ctatataaac agaaaagaaa ggttaattat tggtgttagc
       61 tatataaaga gtaacaacag caatgtatat gaataattaa aggttatgca acgggcaaag
      121 attaaatttc ccagtttaat gacaggttgc tgtacttgcg tatgttgctc ttgccggatg
      181 cggcgtaaac gccttatccg gcctacagtt cattgcaaat ccattcaatt tcaatgcacg
      241 atgtaggcct gataagcgta gcgcatcagg caattttaat gtttgacttc cctgtttaat
      301 cagttactgc tggcgtagct ggagatccag cggcgttttg ctgggttcgc cgccaatttc
      361 tcgcgccagt ttcggcacca gatatcccga caccagtgtc agcaactcac gcataatctg
      421 ccgtgcttcg tcatcactca ccataaaatg cgccgcgccc tgtactttat cgagcacatg
      481 caggtaatac ggcatcacgc ctgcatcgaa caacgcatta ctcaggtttg ccagcgtttg
      541 tgcgttatcg ttcacaccac gtaacagaac gctctggttc agcaaagtaa cgcccgcccg
      601 gcgcaactta gccatcgcct gacggaatgt ttcatctatc tcattggcat ggttgatgtg
      661 attcaccagc aagatttgca gcgtagaatg tgaaaagcgt tcaaccagcg catctgtgat
      721 gcgtgccggg atcacaatcg gcagacgact gtgaatccgc agacgtttga tatgcgggat
      781 ggcttccagt tgtgtgagca accagtccag ctcgtgatct ttcgccatca gcggatcgcc
      841 gccggagaaa atcatctcgt ccagttccgg atgcgcagca acatactcaa gtgcagtctg
      901 ccagttacgc ttgttgccct gattttcggc ataggggaag tggcgacgga agcaatagcg
      961 gcaatttacc gcgcagccgc ctttgaccag caaaagcgcc cggttgtggt atttatgcaa
     1021 caaaccaggc actacgctgt gctgttcttc cagtgggtcg gtggagaatc cgggcgcgac
     1081 gacaaactca tcttgcgagg taattacctg acgcaaaagc ggatcgtccg gattgccttt
     1141 ctccatgcga tcgataaatg agcggggcac acgcagggca aacagctttt tggcgctgcg
     1201 tccggccaac agtttttcgt ccgcgtctat attcaaaaga cgcagaagtt catcaggatc
     1261 ggtcacaaca tcggcaagtt gcgttaacca atcttctctg gatggggtat ttagggttac
     1321 aatatgcgcc attttgtggc ttagctacca attaacaaat ttcagagggc cttatggcaa
     1381 cgtactatag caacgatttt cgtgctggtc ttaaaatcat gttagacggc gaaccttacg
     1441 cggttgaagc gagtgaattc gtaaaaccgg gtaaaggcca ggcatttgct cgcgttaaac
     1501 tgcgtcgtct gctgaccggt actcgcgtag aaaaaacctt caaatctact gattccgctg
     1561 aaggcgctga tgttgtcgat atgaacctga cttacctgta caacgacggt gagttctggc
     1621 acttcatgaa caacgaaact ttcgagcagc tgtctgctga tgcaaaagca attggcgaca
     1681 acgcgaaatg gctgctggat caggcagagt gtatcgtaac tctgtggaat ggtcagccga
     1741 tctccgttac tccgccgaac ttcgttgaac tggaaatcgt tgataccgat ccgggcctga
     1801 aaggtgatac cgcaggtact ggcggcaaac cggctaccct gtctactggc gctgtggtta
     1861 aagttccgct gttcgtacaa atcggcgaag tcatcaaagt ggatacccgc tctggtgaat
     1921 acgtctctcg cgtgaagtaa tgcggttgtg gtgcggcctg caggctgcac catcacttat
     1981 tcaggtcaga gatgatgaaa cgccttatcg ttcttgtttt gcttgccagc acgctgctca
     2041 cgggctgtaa caccgctcgc ggtttcggcg aagacatcaa acatctcggc aactccatct
     2101 cccgcgctgc cagctaattt ttcttctctt ccgaaaaatc atcagatttc catcattttt
     2161 ggcgatgttg tctattatta atttgctata ggcaaacata aataacatta cctaaaagga
     2221 agacgttatg gtgaagaaga caattgcagc gatcttttct gttctggtgc tttcaacagt
     2281 attaactgcc tgcaacacca cgcgtggcgt tggtgaagac atttctgatg gcggtaacgc
     2341 gatttctggt gcagcaacga aagcgcagca ataagcaata acggtacgac agttatgtcg
     2401 tgccgtttgt ttttttgcga tagtcacaaa ggtaatagtt gaaattcccc tgccacctgg
     2461 caaaatatcc gttcaaccat cagctttgca ggacgacctg caaacgcctc ttttcaccgg
     2521 ggacggcccc aattctccgg agcctgatat gtcctggatt atcttagtta ttgctggtct
     2581 gctggaagtg gtatgggccg ttggcctgaa atatacccac ggctttagtc gtttgacgcc
     2641 aagtgttatt actgtgacgg cgatgatagt cagtatggcg ctacttgcct gggcgatgaa
     2701 atcgttaccg gtagggacgg cttatgccgt gtggacgggt attggcgcag tcggcgcggc
     2761 catcaccggc attgtgctgc tcggtgagtc cgctaacccg atgcgtctgg cgagtctggc
     2821 gttaatcgta ttggggatta ttggtctgaa actcagcact cactaactac cgggctgctg
     2881 tacccaaata aacttactga catcaaaccc ttcccgggtc gcgactgcca gcatctcctg
     2941 tttcacttcg tcagaaatgg ttggcgtgcg ggagagtatc cacaggtagt cgcggtccgg
     3001 tccgcaaacc agcgcatggc ggtattcccg atcgagtgca ataacgttat aaccgccata
     3061 gaaaggacca aagaatgaca ctttcagcgc agcgcgagtt ggtgcgccgg taaagtacgc
     3121 tttcccttca ctctgctgcc acatttctct gtcagggtta tagcctttat taatgacatt
     3181 caggccgcca tcatccctca ggctgtatgt tgcggtgact ttttccagtc cacgttcaaa
     3241 gcggtgatca aaacgggcaa tctcatacca ggtaccgaga tagcgtttag cgtcgaaatt
     3301 attcactacg gtcacgccac gcggcggcgt aggagaactg caggcaacga ccagaaatgc
     3361 agctgtcgct gcggcaacga gagggagcag gcgcataaat gtttccttac tggttttttt
     3421 ctaagtgtag atgacagcaa ggaaaagcgc agaaaaggtc cgaaaattcg gacccgatga
     3481 aattttactg tagagcgttg agaatctgcc aggcggcggc gactctcgct ggattgggat
     3541 agtttttgtt agccagcatc actataccca gctctttttc tggaataaac gcgacatagc
     3601 taccaaatcc gccggttgcc cctgttttat gtacccatga tgcgcgtact gcaggagttg
     3661 ggggcgtaat cgcttttacg gggtgtgccg ccagtgcaat tttattgcca ctgccgttaa
     3721 tgatgctgtc aggatttacc ggccagtcca gcatttccca gcccaggccc tgatacatat
     3781 cgccggtttg ccagtagcga gattgtgcca cttgtatccc ttgttgaagc gttttctcat
     3841 tgatatcaag gggttttaaa ttgatttgca cccagcgggc catatcttca atggtcgact
     3901 tcacaccata agcttcagca tctaacgccc ctggcgaaac atgcactgcc ttaccttcgc
     3961 gatatcccca ggcgtaattc ttttcttctg cgggcggtac attaatccac gtatggttga
     4021 gtttgagtgg ctggaagaca cgagtgtgca tcgcctgctc aaaactcaaa ccagacggct
     4081 ttacagccag tgcgccgaac aaaccgatac tggagttggc atacagacgt tgtgttcctg
     4141 gcgcccatac aggctgccag ttttgataga agtgcagcaa gtcgcttgag gatttcacct
     4201 catccggcac ctgcaatggc aggccgccag cggtgtaggt tgcgagatgt aatagcgtga
     4261 tcccgttcca ctgtttagcg gtaagttcag gccagtattt tgttgcggga tcgcttaact
     4321 tgatttcccc tcgagcaata gcgtcgccac caagcacgcc cgtaaatgtt ttgctgaccg
     4381 aacctaactc aaacaacgtt tgctgtgtga cgggctgctt tttggcgatg tccgcatagc
     4441 cccaggtaaa gtaataaggt ttaccctgat aaattaccgc caccgccata ccggggatct
     4501 tttgttgctc tataagcggg gtaattgtgc gatgcacaat atcgttgatt tgttgagggg
     4561 cagcaaatgt ggagcaagag gcggtaatta ataaggcgca gagcgtcgtt ttgaacatag
     4621 ggtctggttt ccatacaaaa cggcccgcca taggcgggcc ggatttacat tggcgatacg
     4681 ttagattgta acgataccaa tcagcgtgac aactgtcagg atagcagcca gaccgtagaa
     4741 aacccatttg cccgcaggca cgtgaatttt cagatcgtgc atcgcgtggt gcatacggtg
     4801 taaaccacac cacagcggca gaacgatcat caggaacagg aatacgcgac caatgaagct
     4861 ctgcgcgaac gccagaacgc gctcgtagct cagcgcatca cccggaaaca gccccagtgg
     4921 cagcagaata cccaccagca ggatcatcac cggcgcaata attgcgctcc acataccacc
     4981 ggccccgaag aggccccaga ataccggttc gtcagaacgc tttggatttg gattaatcat
     5041 ctcaggctcc ttaccagtac agggcaacaa acaggattac gatggtggca accacagtta
     5101 ccgcccagag acttttgata attggctctg gtcccatttt ttcgtctttt acaatgatat
     5161 tggccgcttt cggtgccagt tcaaaccagg ttttggtgtg cagcagagct gccgccagag
     5221 tgatcaggtt aatgatcacg ataaccgggt tttgtaaaaa gtcgacgaat cccgcccagg
     5281 cttccgggcc atttttcagg gcaaacagcc cgaaaatcag ttcaatgctg aaccacacag
     5341 ccggaaccgc cgtgccttcg cgcagcatgt aaaagcgata aaacggcaat tttttccacc
     5401 aggtggacgt cattggccgt acatacggtt tacgtttagt cgtcatgttg cactccttag
     5461 cgtggtttca gggtcgcgat aagaaagtct ttcgaacttt ctactttgcc ctgctgaatg
     5521 gccgcagccg gatcgacgtg tttcgggcag acttcggagc aatagcccac gaaagtacag
     5581 ctccatacgc cgttctggct gttcaactgc gccatacgct ccttcttacc gtggtcgcgg
     5641 ctatcttcgt tataacgatg cgccagcgta atggcagccg gaccgatgaa ctctgggttc
     5701 aggccaaact gcgggcacgc ggcgtagcac agaccacagt tgatgcaacc ggagaactgg
     5761 tgatactttg ccatctgcgc cggggtctgg atgttagtac cctgatccgc ggtgcgggag
     5821 ttgccgatga tgtacggttt gatcgcttcc agactttcga tgaagtgggt catatcgacc
     5881 accagatcgc gttcaatcgg gaagttagct aacgcttcaa ccttcatacc gtcggtgtaa
     5941 tcacgcagga aggttttaca tgccagtttt ggcacgttgt taaccatcat gccgcaggaa
     6001 ccacaaatcg ccatacggca ggaccagcgg taactcaggt ccggtgccag gttgtctttg
     6061 atgtagccca gcgcatccag taatgaggta gttgcgtcat aaggcacttc atagaatgcg
     6121 ctatgcggtg cggtatcgac ttccgggtta tagcgcacca cctcaatttt caggtttttc
     6181 atctcagcca ttcgccttct ccttcttatt ggctgcttcc gccttatcgg ctgcatccgc
     6241 ttcgccaccg taaacgcgtt tagctggcgg cagcgtagta atcttcacgt cgctgtactc
     6301 caggcgagtc gtgccatcag catcgcggaa ggcgagggtg tgtttgagga agttgacgtc
     6361 gtcacgctcg gtgcaacctt cgtccagacg ctggtgtgcg ccgcgggact ctttacgtgc
     6421 cattgcggag tgcgccatac attcagcaac gttcagaccg tggcccagtt caatggtgta
     6481 gagcaggtcg gtgttgaaca cgctggaggt gtcggtgatg cgcacgcgct tgaagcgttc
     6541 ctgcagctct gccagcttgt cgatggtttt ctgcatcagt tccggcgtac ggtagatacc
     6601 gcaaccttct tccattgcca ggcccatttc gtcgcggatc ttcgcccagt tttcgccgcc
     6661 atcctggtta accagatctt tcagacgttg ttcaacgcca gctgcctgcg cttcaattgc
     6721 cgcttcgttg ccattaccgg cagttgctgc acgctctgtc gcttgttcac cggccagacg
     6781 gccgaagacc accagttccg ccagggagtt ggagcccaga cggtttgcac cgtgcagacc
     6841 aacagaggaa cattcaccca cggcgaacag acctttaatg cgggtttcac agttctgatc
     6901 ggtttcgata ccgcccatgg tgtagtgtgc ggtcggacgt accggaatcg gttctttaac
     6961 cggatcgacg ccaacgtacg ctttcgccag ttcgcagatg aacggcagac gttcatgcag
     7021 ttttttctcg ccgaggtgac gcaagtcgag ataaaccaca tcgccacgcg gcgtggagat
     7081 ggtgttgcct ttacgccatt cgtgccagaa ggcctgagaa actttgtcgc gtggacccag
     7141 ttccatatat ttgtttttcg gctcgcccag cggagtttcc gggcccatgc cgtaatcttg
     7201 cagataacgg tagccatttt tgttgaccag aataccgcct tcaccgcggc agccttcggt
     7261 catcaggata ccggaacctg gcagaccggt tgggtgatac tgaacgaatt ccatgtcacg
     7321 cagcggaacg ccgtggctta gcgccatacc cataccgtca ccggtaacga tgccgccgtt
     7381 ggtgttgtaa cgataaacgc gacccgcacc gccagtagcc ataacgaccg cgttagcacg
     7441 gatctgcacc agcgtgcctt ccatcatgtt cattgctacc aggccgcgaa catgaccatc
     7501 atcaaccaga atatccagca cgaaatgttc gtcaaaacgc tggatctgcg ggaattgcag
     7561 agaggtctgg aacagcgtgt gcagcatatg gaagccggtc ttatcggcgg cgaaccaggt
     7621 gcgctcgatt ttcatgccgc cgaagcgacg tacgttgacg ctaccatccg ggcgacggct
     7681 ccatgggcac ccccacagtt ccagttgggt catttcggtt gggcagtggt ggacgaaata
     7741 atccacgaca tcctgctcac acaaccagtc gccacccgct actgtatcgt gaaagtgata
     7801 ttcgaagctg tcatgatcct gcgcgacagc ggcggagccc ccttctgcag caacggtatg
     7861 gctacgcatc gggtatactt ttgagattag tgcgattttt gcatttggat ttgcctgcgc
     7921 ggcagcaatt gcagcacgta atcccgcgcc accggcgcct acaatggcaa gatcggcttg
     7981 aaaggtttgc acgacattcc tccagattgt ttttatccca cagccccgta cttcagggta
     8041 agtgcctgaa agttacggtc tgcgaacgct attccactgc tcctttatag gtacaacagt
     8101 atagtctgat ggataagtct gaaatttgac gagatcgatt tttttagtgc gcaagagggc
     8161 taaattatca ctgaagatga ttaatttaat tactaaacca tcagatcgtg ctttttttag
     8221 tcactgccca ccgctgtttg attcctgcgt aaaatttgtc tcgccgtcgc gttgcgagta
     8281 gacttcgtgc ccttgtcaaa aactggagat ttaactatga gcgaaacggc atcctggcag
     8341 ccgagcgcat ccattcctaa cttattaaaa cgcgcggcga ttatggcgga gatccgtcgt
     8401 ttctttgccg atcgtggagt gctggaggtg gagacgcctt gtatgagcca ggcgacggta
     8461 accgatattc atttggtccc gtttgagaca cgtttcgttg gccccgggca ttcgcagggg
     8521 atgaatctct ggttaatgac cagcccggaa taccatatga aacgcctgct ggttgctggt
     8581 tgtgggccgg tattccagct gtgccgcagc ttccgtaatg aagagatggg gcgttatcac
     8641 aaccctgagt tcactatgct ggagtggtat cgaccgcact atgatatgta ccggttgatg
     8701 aacgaggtgg acgatctctt acaacaggtg ctggactgcc cggcagcaga aagcctttct
     8761 tatcaacaag ctttcttacg ttatctggaa attgacccgc tctctgccga caaaacgcaa
     8821 ctgcgggaag tggcagcgaa actggatttg agcaacgttg cagataccga agaagaccgc
     8881 gacacgttgc tacaattgct gtttaccttt ggtgtagagc caaatattgg taaagaaaaa
     8941 ccgacctttg tgtaccactt tccagccagc caggcatcac tggcgcaaat cagtaccgaa
     9001 gatcatcggg tcgctgaacg ctttgaggct tattataaag gtattgagct ggcgaatggt
     9061 ttccatgaat tgacggatgc ccgtgagcag caacaacgct ttgaacaaga taaccgtaag
     9121 cgcgcggcgc gtggtttgcc gcagcacccc attgaccaga atctgattga agccttgaaa
     9181 gtcggtatgc ctgactgttc cggcgtggca ttaggcgttg atcgtctggt gatgttggcg
     9241 ctgggcgcgg agacactggc tgaagtcatc gcctttagcg ttgaccgggc ataattctga
     9301 aaaattactg aattaacagc gaagaatggc gtgataagcg ccattctgta gcatatgttt
     9361 tcatttttct gttaaaggcc acaatttaag actggatatt tgatatcatc caggtatcaa
     9421 ttctgtattg gtttttacgc tgcccgctca atgttgggca gtgcagttca gtgggcgcgt
     9481 tttatacacg cgctgaaatg aaggatggtt tcatgcctca cacgataaaa aagatgagtc
     9541 tgataggact catattgatg atctttactt ccgtatttgg atttgccaat agcccatcgg
     9601 cttattactt aatgggttat agtgcgattc ccttttatat attttctgca ttgttattct
     9661 ttattccatt cgccttaatg atggctgaaa tgggagctgc ttatcgcaaa gaagaaggtg
     9721 gtatctattc ctggatgaat aatagtgtcg gaccacgttt tgccttcatt ggtacgttta
     9781 tgtggttttc ctcttatatc atctggatgg tgagtacctc cgcgaaagtt tgggtaccgt
     9841 tctcaacatt cctctatggt agcgacatga cccagcactg gcgtattgcc ggactggagc
     9901 ctacgcaggt ggttggtctg ctggcagtgg catggatgat tctggtcacc gtcgttgctt
     9961 ctaaggggat taataaaatt gcccgcatta ctgcggtggg cggtattgca gtaatgtgtc
    10021 tgaatttagt attgctgtta gtaagcatta ctattttgtt attaaatggt gggcatttcg
    10081 cgcaggatat taatttcctt gcatcaccga acccgggtta tcagtccggt ctggcaatgc
    10141 tatcgtttgt ggtatttgct atttttgcct atggcggaat tgaagcggtt ggtggtctgg
    10201 tcgataaaac ggaaaatcca gaaaagaact ttgccaaagg tattgttttt gccgctattg
    10261 ttatttcaat cggttattcg ctggcaatat ttttatgggg cgtcagcaca aactggcagc
    10321 aggtattaag taatggttcc gttaacctcg gcaatattac ctatgtgctg atgaagagcc
    10381 tcggggtgac gctgggtaac gcactgcatt tgtctcctga agcgtcattg tcgctgggcg
    10441 tatggtttgc gcgtattacc ggactttcga tgttcctcgc ttataccggt gcgttcttta
    10501 cgctttgcta ttcaccgctg aaagccatca tccaggggac gccgaaagca ttgtggccgg
    10561 aaccgatgac gcgcctgaat gcgatgggga tgccgtctat cgccatgtgg atgcagtgcg
    10621 ggttggttac tgtcttcatc ctgctggttt cgtttggtgg cggtaccgca tcggcgttct
    10681 ttaacaagct gacgctgatg gcgaacgtgt ctatgacgct tccttacctg ttcctcgcgc
    10741 tggctttccc attctttaaa gcacgtcagg atctcgacag accatttgtg attttcaaaa
    10801 cgcgtatgtc ggcaatgatt gcgacggtgg ttgtcgttct ggtggtgaca tttgcgaacg
    10861 tcttcaccat tattcagcct gtggttgaag ccggagactg ggacagcaca ttgtggatga
    10921 ttggcggccc tgtcttcttc tcgctgttag cgatggcgat ttaccagaac tattgcagtc
    10981 gcatggcgaa taaacctgag ttagctctcg actgatcata tgaaatcctg caactcatcg
    11041 tctctccggc gatgagttgt caggaagtaa tgcgtatgga tgatactctc cagggccggt
    11101 gataactgaa gatgatgtta agctgctaaa tgagccacgt tgacatcatt tggcagcttc
    11161 atgcattaca aacttcccgc ctgacgacct ttacccgcag acgtcagcgt tctccccgtt
    11221 tgtttaccgt tgaggctttc cagacgcatc tggaagggcg ggaatggcat atcgataccg
    11281 tgggcgtgga agccagccag aatcagctgg tggatctcat ggcgtagcgg catacggtga
    11341 cccatctcag cggcgtaaat acgcagctcg aaaatctgaa tcccctgttg cagatccacc
    11401 aggaagactt ccggtgccgg gttgtcgatt accagcgagc agcgacgcgc tgcggtgagc
    11461 aggatttccg tcacttcttc actgttggca tcggcagggg ccggaatcgt caacaccacg
    11521 cgcgtgaccg agtcagagag cgaccagttg ataaactgct cggtaataaa cgccttgttc
    11581 ggcacgatta tctctttacg gtcccagtcg ctgatggtgg tggcgcgggt gttaattttt
    11641 gtcacgctac cagtaagatc gcggatcgtt accgtatcgc caatgcgaat cggtttttcg
    11701 aacaggatga tcaggccaga gataaagttg gcgaaaattt cctgcaaacc aaaaccgaga
    11761 ccaacaccga gcgcggcgac cagccactgc aatttcgacc actcaatacc aatcatcgag
    11821 aagccgacca gcccgccaat caacatcagc agatatttgg tgatggtggt gatggcgtag
    11881 ccggtacccg gcgttaaatc caggtgctgc aaaatcgcca gttccagcag cgcgggcaga
    11941 ttgcgcacca gctgcgtggt gatgataaac accagaatgg caatcagcac cgcaccgagg
    12001 gtaatcggct ccagactttc tacgccctgt accgtggagg tgacatccca cagcgaaata
    12061 ttttcgagga agccgaaagc ggaatggatt tctgaccaca gcacaatgac cgaaagcagg
    12121 gcgatcaaca tcaaaattga gcgcaccagc cgcaaggact gcgcactgat ggcatcgaga
    12181 tcgacttcgc tttcatcgac ttcaattgcc ccttccgggc tactgtgatg atgtgcttcc
    12241 tcttcgccac gcgcacgttg cgctaacatc tctgcccggc gatgcttcgc ccggtcaaac
    12301 gccagcctgc gtcgttggat cagcatccag cggcggataa cgtgataaac caccagtagc
    12361 aggaaccaga tggcaaccga ggtttcaagc ctcgctaaca gcgcctgtgc cgttgccaga
    12421 taacccaccg ccgacgccag aatggcaacc agtggcgcgc caatcatcat gttccacaac
    12481 atatggttgg taatattgtc gccgctgcct tctttgttga gatacagcgg gatcccggcc
    12541 tttttcaggc tgagggtgac caccgccagc gcaccgcaaa tgaggataaa gcaaagccgt
    12601 cccagcgaac cggaaaattc gcggtcgtcg agattatcga acatcatcag cgccataatc
    12661 agtggcacaa taagcccgat gctcatcagg tagtagcgca tcccacggga aacacgttcg
    12721 cgcggccaac caaaatgagc gataaacaag ccgttcgggc gggcaaaggt agcgcaaatc
    12781 atcactaccc acagcagcgg cacggtggct gtgacgccat caccaatcgc gaccgccagc
    12841 ggataaggcc acgcctcgcg caagccgtaa cccagcgtca tccacagcac cggcagcggt
    12901 gacgcgacga gaatcgacca gaaaagagtg cgtaacgtca gccagaagtg atcctgagtc
    12961 actttgccga ctttcgccgc cgaacgttcg agaaaacggg taaaatagcg gcgcgagtaa
    13021 atactgcaac cgaccagaat caacgcgcca aacagcggca aaatcgtctc tttgctggtc
    13081 agcatcatca cactggcttt gcccaactga ctgaaggtgt ccagcgaaat gagacgacgc
    13141 agatcctggg cgatttccag cggccaggcg atggtcatcg ggcgcacgtc agaggtccag
    13201 aacagatagc ggtgcgtcgc ttcgttcacc tctttcagcg catcctccag ttgcccgtta
    13261 gagactttca gcttggtcag ttccagcagt agtgtgtcgc caccctgcaa caatgagttc
    13321 agcaactcac gctgagtgcg tagctgtgct tcgagaatac ggttttgctc ggcagtcagc
    13381 ggctgaccgt cggcctgatg aatttgccgt agcagcggct gtttattaag cagatcctca
    13441 taacgtaacc gttgcacacg caactgcgcc atttcggtat caagctgttg tggtttcggc
    13501 atttccggca gccgtgccac ctgcgcccgc aacgcttcac cgagcagatt ggacgatccc
    13561 agccattgcg actgttcacg cagcgtattc aacgcctgcc ggacctgtaa cgtctggctg
    13621 gcagcctgac gctgttgcga ggcaacgaga tccatccgct gcgcctgttg attcaaagcc
    13681 gccgatagtt cgcggttaat tttgaattgc gcgacgatat ctttcggcaa atcggcactg
    13741 ttttctgcta gtaattcggt actttccagc gcccgctccg cctcaagctg acgttggctg
    13801 ttcaactgat tacgtaaggc ctgcaaatac gcatccagtt gctggctctc tttttccgcc
    13861 agctcagagc gcaagcgcgc taattcctgg cggttattgg cagacagctg cgccagttcc
    13921 agttcatcaa cgagcgcttt caggcgtgcg gagtcagact gcaacgcgaa attttgtgcc
    13981 tgattgagcg gggtattgcc agtaagcgtt cccaggcggc gctcgatctc atttaactga
    14041 cggcgggcgt cggtttgctg ttgcggcagt tgattcagcg aatcggcaat ctcgcgggcg
    14101 cgctcctgct cttgctgggc ctgacggctt ttatccagca actggctgct gacctggaga
    14161 atttcctgat tcagcgcgtc ggtagacatc cccggcgaca cgctgcgcgg ctcgtcacgc
    14221 atgttgttta attgtgcgcg cagagtagcg gagagtttcg gataattatc aatgatttct
    14281 tgatattgct tgatgcgctc aagggaacct tttcgttcct caagcgcatt taaggcagac
    14341 tggagcgcct ctacgacttc cggctgtgcg ggtttcgccg cttttgcctg ctccagttct
    14401 tgtgagattt gtttgctatc gggggccgtc gcggcgtacg ccccccaact gaggcaccag
    14461 gccatcagaa aagtgataat caggcgcacg tcagcgtttc ctttgatgga ttagacctgg
    14521 tcttttttat cgtcaaccaa tgggctggcg tcgtgttctg cttcgatctc ttcagcagga
    14581 agtggagctg gttcagcgtc tggcgtaaca aaggtttcgg tagatactgc cagcggctgg
    14641 ccaattttcg tgacagacag gctttccagt tgctcaacca gattaacttt acccggtgca
    14701 aacaggttga taacggtgga accgagttta aaacgaccca tttcctggcc tttcagcagt
    14761 gccacagaac cgtcgttttc cccggcaggc caggtccagc gcttgatgat gccttcgcgc
    14821 ggcggcgtaa tggtgcccgc ccagacagtc tcaatgctgc ctacaatcgt cgcaccgacc
    14881 agaatctgcg ccattgggcc aaattcggta tcgaacaggc agatcacgcg ttcgttacgg
    14941 gcaaacagat tcggcacgtt ctgagccgtg agatggttaa cggagaagag atcgcccggc
    15001 acgtagatca tctcacgcag aataccgttg cacggcatgt gtacgcggtg gtagtcacgc
    15061 ggggagaggt aagtggtcac aaacgtaccg ttgcggaaca ggtccgccat cagatagttg
    15121 cctgccagca gggcttcgag gctgtagttg tggcctttgg cttgcaggat tttatcttct
    15181 tcgattttac ccaactggct gataacgcca tcggcaggca taaccaggac attcggatcg
    15241 gtatcgattg ggcgtacttc gtcacgcagc ggacggacaa agaattcgtt aaaggtgcgg
    15301 tagctggcgg tgtccggctt ttgcgcctct ttcatgtcga ccttgtagta tttaacgaac
    15361 agatcgataa ccagttttgt cagccatcct gcccgcttgc ttgcgcccca acccgccagg
    15421 cgagtaagcc atagtttcgg cagaatgtac tgtagcgaaa gtttaaatga atttaacaag
    15481 gtagcctcca ggccattgtt ttgtcgttcc tgatccggcc tacatgccgg atcctgaaaa
    15541 aaaggggacg attctaacga cggttagctt aattgtcagt catccgtatc agaaaagttt
    15601 ttacgcgttt ttacctgcgc catgctttcc agaatacggt gatagttttc gaaacgggtt
    15661 tccgcgattt tcccttcctc aaccgcttcc cggatagcgc agcccggatc ggtatcgtgt
    15721 ttgcaatcgc gatatttaca cagacctaaa tagtcatgga attcgacaaa gccctgagtg
    15781 atttgttccg gctccaggtg ccagaggccg aactcacgca caccagggga atcaatcaca
    15841 tcaccgccgt gcgggaagtg atacagccga gcggcggtcg tggtgtgctg accgagcccc
    15901 gagttgtcag agacatcgtt ggtcaggatc tctttttgca gccccagcaa cgcgttcagc
    15961 aggctggatt taccaacgcc agactgcccg gcaaaaatgc tgatgcgccc ggtcaacgcc
    16021 tcttccagcg gttttagccc atcctgagta tggctggaaa ccatcaatac gcgataaccg
    16081 atattgcggt agatatccat ctgctcgttg acgaactcca taccttcgtc gtccagcagg
    16141 tctatcttgt tgagcacaat aatcggctca atctgcaagg tttcgcaggc caccaggtaa
    16201 cggtcgataa tattgagtga cagctccggc aaaatggcgg agacaatgac aatctggtcg
    16261 atgttggcgg cgatgggttt cacgccgtca tagaaatccg ggcgtgttaa caccgaggtg
    16321 cgctcatgca ccgcttccac gatccctttg acatttacgc cttccgccgc cggtttaccc
    16381 ggacgccaga ctacgcggtc gccggttacc agtgaacgga tggtgcggcg aatattgcag
    16441 cggtgaacgt cgccatcggc ggattccaca tcagcgtgca taccaaagcg gctgatgacg
    16501 ataccttcat caggctcgcc aaacagattg tcgtcgtagt cgggcttctc cttagacgtt
    16561 ttaagacgac gctggtgatt ggcgttcacg cggcgctgct ggcctttgga gagtttattt
    16621 ttactcaatc gtacagactc ctggtcgccc ctgatgggca aaacatctat gatacacgca
    16681 attgtggatc aatatagtca ctgtgaatgg gtggaaaata gcatgagtgc caatgaaaac
    16741 aacctgattt ggatcgatct tgagatgacc ggtctggatc ccgagcgcga tcgcattatt
    16801 gagattgcca cgctggtgac cgatgccaac ctgaatattc tggcagaagg gccgaccatt
    16861 gcagtacatc agtccgatga acagctggcg ctgatggatg actggaacgt gcgcacccat
    16921 accgccagcg ggctggtaga gcgcgtgaaa gcgagcacga tgggcgatcg tgaagctgaa
    16981 ctggcaacgc tcgaattttt aaaacagtgg gtgcctgcgg gtaaatcgcc gatttgcggt
    17041 aacagcatcg gccaggatcg tcgcttcctg tttaaataca tgccggagct ggaagcctac
    17101 ttccactacc gttatctcga tgtcagcaca ctgaaagagc tggcgcgccg ctggaagccg
    17161 gaaattttgg atggttttac caagcagggg acgcatcagg cgatggatga tatccgtgaa
    17221 tcggtggcgg agctggctta ctaccgcgag cattttatca agctgtaaaa tttttcctgg
    17281 tcacgtaagc gtcggaatcc acttgcggcg gcgtgatttt gacgctaaat tgaacgcctt
    17341 gctgataatt tcagcacttg agataaaaac tcaaaaaaaa ttttttaggg gggttgcaga
    17401 gggaaagatt tctcgtataa tgcgcctccc gtaacgacgc agaaatgcga aaattacgaa
    17461 agcaaaatta agtagtacgc gggaatagct cagttggtag agcacgacct tgccaaggtc
    17521 ggggtcgcga gttcgagtct cgtttcccgc tccaaaattt gaaaagtgct gcaaagcaca
    17581 gaccacccaa gcgggaatag ctcagttggt agagcacgac cttg
//
LOCUS       DAPGOF010000098        17612 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.098, whole genome shotgun sequence.
ACCESSION   DAPGOF010000098 DAPGOF010000000
VERSION     DAPGOF010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.098"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            49..1188
                     /gene="arnB"
                     /locus_tag="RHV13_002906"
     CDS             49..1188
                     /gene="arnB"
                     /locus_tag="RHV13_002906"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HDV1242421.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALAQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1192..2160
                     /gene="arnC"
                     /locus_tag="RHV13_002907"
     CDS             1192..2160
                     /gene="arnC"
                     /locus_tag="RHV13_002907"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HDV1242422.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREYGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2160..4142
                     /gene="arnA"
                     /locus_tag="RHV13_002908"
     CDS             2160..4142
                     /gene="arnA"
                     /locus_tag="RHV13_002908"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HDV1242423.1"
                     /translation="MKTVVFAYHDMGCLGIEALLSAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPDVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVTRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNNQPACAARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4139..5029
                     /locus_tag="RHV13_002909"
     CDS             4139..5029
                     /locus_tag="RHV13_002909"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HDV1242424.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQAHSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5029..6681
                     /gene="arnT"
                     /locus_tag="RHV13_002910"
     CDS             5029..6681
                     /gene="arnT"
                     /locus_tag="RHV13_002910"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HDV1242425.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVAMFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            6678..7013
                     /gene="arnE"
                     /locus_tag="RHV13_002911"
     CDS             6678..7013
                     /gene="arnE"
                     /locus_tag="RHV13_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HDV1242426.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7013..7399
                     /gene="arnF"
                     /locus_tag="RHV13_002912"
     CDS             7013..7399
                     /gene="arnF"
                     /locus_tag="RHV13_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HDV1242427.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIATLLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7393..7659)
                     /gene="pmrD"
                     /locus_tag="RHV13_002913"
     CDS             complement(7393..7659)
                     /gene="pmrD"
                     /locus_tag="RHV13_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HDV1242428.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASCYSPDEWERQCKVAGKTQ"
     gene            complement(7769..9124)
                     /gene="menE"
                     /locus_tag="RHV13_002914"
     CDS             complement(7769..9124)
                     /gene="menE"
                     /locus_tag="RHV13_002914"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HDV1242429.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AVQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPIELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLDEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9121..10083)
                     /gene="menC"
                     /locus_tag="RHV13_002915"
     CDS             complement(9121..10083)
                     /gene="menC"
                     /locus_tag="RHV13_002915"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HDV1242430.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRTAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(10083..10940)
                     /gene="menB"
                     /locus_tag="RHV13_002916"
     CDS             complement(10083..10940)
                     /gene="menB"
                     /locus_tag="RHV13_002916"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HDV1242431.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10955..11713)
                     /gene="menH"
                     /locus_tag="RHV13_002917"
     CDS             complement(10955..11713)
                     /gene="menH"
                     /locus_tag="RHV13_002917"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HDV1242432.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEVFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LYGVIVEGGHPGLQNAEQRAERQRSDRQWVQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11710..13380)
                     /gene="menD"
                     /locus_tag="RHV13_002918"
     CDS             complement(11710..13380)
                     /gene="menD"
                     /locus_tag="RHV13_002918"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HDV1242433.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQLLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRVCDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETVLADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13469..14764)
                     /gene="menF"
                     /locus_tag="RHV13_002919"
     CDS             complement(13469..14764)
                     /gene="menF"
                     /locus_tag="RHV13_002919"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HDV1242434.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTLLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14843..15148)
                     /gene="elaB"
                     /locus_tag="RHV13_002920"
     CDS             complement(14843..15148)
                     /gene="elaB"
                     /locus_tag="RHV13_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HDV1242435.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15203..15664)
                     /locus_tag="RHV13_002921"
     CDS             complement(15203..15664)
                     /locus_tag="RHV13_002921"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV1242436.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDNLEPVVIGRVIVSEALRGEKVGQQLMSKTLDT
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEIYEEDGIPHIGMAREVIQA"
     gene            15729..16646
                     /gene="rbn"
                     /locus_tag="RHV13_002922"
     CDS             15729..16646
                     /gene="rbn"
                     /locus_tag="RHV13_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HDV1242437.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            16844..>17612
                     /locus_tag="RHV13_002923"
     CDS             16844..>17612
                     /locus_tag="RHV13_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HDV1242438.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTDELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKAENILYHIVRNPTNDTLSIIKLIKNACLKLYNLAHTATN
                     SHLKPTAPDNSDDLLFKKLSSPSKLMTIIGDEIPLISEKQSLSKVLLNDENNELSDGT
                     NFWDKNRQLTTDEIDCYLQKIAANAKNTQVNYPTGLYVPYSTRTHLEDALNDNIKSDP
                     SWPKEVQLFPINTGGHWILVSLQKIVNEKNNTQQIKCV"
BASE COUNT         4174 a         4739 c         4433 g         4266 t
ORIGIN      
        1 ttatagcaaa tataagtgga caggtattca atggcggaag gaaaagcaat gtcagaattt
       61 ttgccttttt cgcgaccagc aatgggcgtg gaggaactcg ctgcagttaa agaggttctc
      121 gaatccggtt ggatcacaac cggtccgaag aatcaggcgc ttgaacaagc cttttgccag
      181 ttgacaggaa atcagcatgc catcgcggtc agttcagcca ccgccggaat gcatatcacg
      241 ctaatggcgt tggaaattgg caagggcgat gaagtgatta cgccttccct gacctgggtt
      301 tcaaccctca atatgatttc cttgctgggt gcaacgccgg taatggtgga tgtcgatcgc
      361 gatacgctga tggtcacgcc tgaagcgatc gaatcagcca ttacgccacg cactaaagcc
      421 atcataccgg tgcattatgc cggtgcgcca gcagatattg acgccattcg cgccattggc
      481 gaacgttacg gcatcgcagt tatcgaagat gctgcccatg ccgtaggtac gtattacaaa
      541 gggcgacata ttggcgcaaa aggtaccgct attttttcat ttcatgccat taaaaatatt
      601 acctgtgctg aaggtggcct gattgtaact gataatgaaa accttgcccg ccagctacgg
      661 atgctgaaat ttcacggcct gggtgtcgat gcctatgaca gacaaacctg gggccgtgca
      721 ccgcaggctg aagtcttaac gccgggctat aagtacaatc tgaccgatat taacgccgcg
      781 attgccctgg cacagttagc caaattagag cacctcaaca cccgtcggcg cgaaattgcc
      841 cagcaatatc agcaagcact ggcagctctc ccctttcagc cattaagcct tcccgcctgg
      901 ccgcacgttc acgcctggca tctgtttatt attcgtgtcg atgaacaacg ttgtggtatc
      961 agtcgcgatg cgttgatgga agcgttaaaa gaaagaggta ttggtaccgg gttacatttc
     1021 cgcgccgctc acacacaaaa atattatcgc gagcgttttc ccacgctgtc gttaccgaat
     1081 accgaatgga atagcgaacg catctgttcg ttgccgctgt tcccggatat gactaccgcc
     1141 gatgccgacc gcgtcatcac agcccttcag caactcgcag gacaataagc catgtttgaa
     1201 atccaccctg ttaagaaagt ctcggtggtt attcccgttt ataacgagca ggaaagctta
     1261 ccggaattaa tcaggcgcac caccacagcc tgtgaatcac tggggaaaga gtatgagatc
     1321 ctgctgattg atgacggcag tagcgataat tccgcgcata tgctggtcga agcctcacaa
     1381 gcggagaaca gccatattgt gtctattttg cttaaccgca attatgggca gcattcagcg
     1441 attatggcgg gattcagtca cgttactggc gacttaatta ttaccctcga tgccgatctc
     1501 cagaatccgc cagaggagat cccccgtctg gtggcaaaag ccgatgaagg ttacgacgtg
     1561 gtagggactg tacgccagaa ccgccaggac agctggtttc gcaaaaccgc ttcgaagatg
     1621 attaaccggc ttattcagcg caccaccggc aaagcgatgg gtgactacgg ttgtatgttg
     1681 cgcgcctatc gccgtcatat tgtcgatgcg atgttgcact gccatgaacg cagcaccttt
     1741 atcccgattc tggcgaatat cttcgcccgc cgtgccattg aaattccagt gcatcatgcc
     1801 gaacgtgagt atggtgaatc caaatacagc tttatgcgcc tgattaattt gatgtacgac
     1861 ctggtgacct gccttaccac aacgccgcta cgtatgctga gtctgctcgg cagcattatt
     1921 gcgattggag gttttagcat tgcggtgctg ctggtgattt tacgcctgac cttcggacca
     1981 caatgggcgg cagaaggcgt ctttatgcta tttgccgtgc tgtttacttt tattggcgct
     2041 cagtttatcg gcatgggatt actcggtgaa tatatcggca ggatctacac cgatgtccgc
     2101 gcccgccccc gctattttgt tcagcaagtt atccgtccat ccagcaagga aaatgaataa
     2161 tgaaaaccgt cgtttttgcc taccacgata tgggatgcct cggtattgaa gccctgctgt
     2221 ctgccggtta cgaaattagc gccattttta cccatactga taatcccggt gaaaaagcct
     2281 tttatggttc ggtggctcgt ctggcggcgg aaagaggcat tccggtttat gcgccggata
     2341 acgttaatca tccgctgtgg gtggaacgca ttgcccaact gtcgccagat gtgattttct
     2401 ctttttatta tcgccatctt atttgcgacg aaattttgca gctcgctccc gcaggtgcat
     2461 ttaatctgca cggttcgctg ttaccaaaat atcgtggtcg cgcgccgctg aactgggtgc
     2521 tggtgaacgg tgaaacagaa actggcgtta cattgcaccg aatggtgaca cgtgccgatg
     2581 ccggagccat tgtggcgcaa ctgcgcgttg ccattgcgcc agatgatatc gccattacgc
     2641 tgcatcataa attgtgccat gctgcgcgcc agctactgga acagacatta cccgccatta
     2701 aacacggtaa tattctggaa atcgcccagc gcgaaaacga agccacctgt tttggtcgca
     2761 gaacgccgga tgacagtttc ctcgaatggc ataagccggc atccgtactg cacaacatgg
     2821 tacgtgccgt tgccgatccg tggccgggtg ccttcagcta tgttggcaat cagaaattca
     2881 ccgtctggtc gtcgcgtgtt catcctcatg ccagcaaagc acagccgggg agcgtgattt
     2941 ctgttgcgcc actgctgatt gcctgtggcg atggcgcgct ggaaatcgtc accggacagg
     3001 cgggcgacgg cattactatg cagggctcgc aattagcgca gacgctgggc ctggtgcaag
     3061 gttcacgctt gaataaccag cctgcctgtg ccgcccgacg ccgtacccgg gtactcatcc
     3121 tcggggtgaa tggctttatt ggcaaccatc tgacagaacg cctgctgcgc gaagatcatt
     3181 atgaagttta cggtctggat attggcagcg atgcgataag ccgttttctg aatcatccgc
     3241 attttcactt tgtcgaaggc gatatcagta ttcattccga atggattgag tatcacgtca
     3301 aaaaatgtga tgtcgtcttg ccgctggtgg cgatagccac gccgattgaa tatacccgca
     3361 acccgctgcg cgtatttgaa ctcgattttg aagagaatct gcgcattatc cgctactgcg
     3421 tgaagtaccg taagcgaatc atcttcccgt caacttcaga agtttatggg atgtgtagcg
     3481 ataaatactt cgatgaggac cattctaatt taatcgtcgg cccggtgaat aaaccacgct
     3541 ggatttattc ggtgtcaaaa caattacttg atcgggttat ctgggcctat ggcgaaaaag
     3601 agggtttaca gttcaccctc ttccgcccgt ttaactggat gggaccacga ctggataacc
     3661 ttaatgcggc acgaatcggc agctcccgcg cgattacgca actcattctc aatctggtag
     3721 aaggttcacc gattaagctg attgatggcg gaaaacaaaa acgctgcttt actgatattc
     3781 gcgatggtat cgaggcgtta taccgcatta tcgaaaatgc gggaaatcgc tgcgacggcg
     3841 aaattatcaa cattggcaat cctgagaacg aagcgagcat tgaggaactg ggcgagatgc
     3901 tgctggcgag cttcgaaaaa catccgctgc gccatcactt cccaccgttt gcgggctttc
     3961 gtgttgtcga aagtagcagc tactacggca aaggatatca ggacgtagag catcgtaaac
     4021 cgagcatccg caatgcccgc cgctgcctgg actgggaacc gaaaattgat atgcaggaaa
     4081 ccatcgacga aacgctggat ttcttcctgc gcaccgttga tcttacggat aaaccatcat
     4141 gaccaaagtc ggcttacgca ttgatgtcga tacctttcgt ggcacccgtg aaggcgtgcc
     4201 gcgtctgctg gaaatcttga gtaagcataa tattcaggcc agcatttttt tcagcgtcgg
     4261 cccggacaat atgggccgcc atctctggcg actggtgaag ccacagtttt tgtggaagat
     4321 gctgcgctca aacgcggcat cgctttatgg ctgggatatt ttactggcag gaacggcctg
     4381 gccaggtaaa gagattggtc atgccaatgc cgatatcatt cgtgaagcgg ctaaacatca
     4441 cgaagtcggc ctgcacgcct gggatcacca tgcctggcaa gcccatagcg gtaactggga
     4501 tcggcaaaca atgatcgacg atattgcacg cggtcttcgc actctggaag agattatcgg
     4561 tcaaccggta acctgttctg ccgctgcggg ctggcgtgcc gaccagaagg tgatcgaagc
     4621 aaaagaagcg ttccatttgc gctacaacag cgattgtcgt ggggccatgc cgttccgtcc
     4681 actgctcgaa tcaggaaacc ctggcactgc gcaaattccg gtgacattac ccacctggga
     4741 cgaagtgatt ggtcgggatg tgaaagcaga agattttaat ggttggttac tcaaccgcat
     4801 cctgcgagat aaaggcacgc cggtttatac cattcatgca gaagttgaag gctgcgctta
     4861 tcagcataat tttgtggatc tcctcaaacg cgcagctcag gaaggcgtga cattttgccc
     4921 tttaagcgaa ctgttatcag agacgttgcc gctcggacaa gttgttcgcg gaaatattgc
     4981 cggacgtgaa ggctggctgg gttgccaaca aattgcgggt agtcgctgat gaaatcggta
     5041 cgttacctta tcggcctctt cgcgtttatt gcctgctatt acctgttacc gatcagcacg
     5101 cgtctgctct ggcaaccaga tgaaacgcgt tatgcggaaa tcagtcgaga aatgctggca
     5161 tccggcgact ggattgtgcc ccatctgtta gggctacgtt atttcgaaaa acccattgcc
     5221 ggatactgga ttaacagcat tgggcaatgg ctatttggcg cgaataactt tggtgtgcgg
     5281 gcaggcgtta tctttgcgac cctgttaact gccgcgctgg tggccatgtt tactctgcgc
     5341 ttatggcgcg ataaacgtct ggcattactc gccacagtaa tttatctctc attgtttatt
     5401 gtctatgcca tcggcactta tgccgtgctc gatccgttta ttgcattctg gctggtggcg
     5461 ggaatgtgca gcttctggct ggcaatgcag gcacagacgt ggaaaggcaa aagcgcagga
     5521 tttttactgc tgggaatcac ctgcggcatg ggggtgatga ccaaaggttt tctcgccctt
     5581 gccgtgccgg tattaagcgt gctgccatgg gtagcgacgc aaaaacgctg gaaagatctc
     5641 tttatttacg gctggctggc ggttatcagt tgcgtactga cggttctccc ctggggactg
     5701 gcgatagcgc agcgggagcc tgacttctgg cactattttt tctgggttga gcatattcaa
     5761 cgctttgcac tggatgatgc ccaacataga gctccgttct ggtactacgt gccggtcatc
     5821 attgccggta gcctgccgtg gctgggatta ctccccggtg cactgtacac aggctggaaa
     5881 aaccgcaagc attccgcaac cgtctatttg ttgagctgga cgataatgcc gctgctgttt
     5941 ttctccgtcg ctaaaggcaa attgcccacc tatattcttt cttgctttgc acctctggca
     6001 atgctgttgg cgcattacgc tttgctggca gcaaaaaata atcctctggc gctgcggatt
     6061 aatggctgga ttaacatcgc ttttggcgtc actggcatta ttgccacatt tgtggtctcc
     6121 ccctggggac caatgaacac gccggtgtgg caaaccttcg agagctataa agtcttttgt
     6181 gcctggtcga ttttttcgct atgggcattt ttcggctggt acaccttaac aaacgtcgaa
     6241 aagacctggt cttttgccgc gctttgcccg ctggggctgg cgttgctggt aggattttca
     6301 attcctgaca gagtcatgga aggaaaacat ccgcaatttt ttgtcgagat gacacaagaa
     6361 tcacttcagc caagccgcta tattcttact gatagcgtcg gtgttgccgc aggtctggca
     6421 tggagcctgc aacgcgatga catcatcatg tatcgccaga caggtgagtt gaaatacggc
     6481 cttaattatc cggatgcgaa agggagattt gtcagcggtg atgagttcgc aaactggctt
     6541 aatcaacatc gtcaggaggg gattattaca ctcgtgcttt cggttgaccg cgatgaagat
     6601 atcaacagtc tcgccattcc gcccgcagat gccatcgatc gtcaggagcg tctggtgctg
     6661 attcagtatc gtcccaaatg atctggctaa cattagtctt tgccagcttg cttagcgttg
     6721 ccgggcagtt gtgtcagaaa caggcaacct gctttgtggc gataaacaaa cggcgcaaac
     6781 atatcgtgct gtggctggga ctggcgctgg cttgtcttgg tcttgccatg gtgctctggc
     6841 tgctggtctt gcagaacgta ccggtaggca ttgcctaccc gatgttaagt ctgaattttg
     6901 tctgggtgac gctggctgca gtaaaactgt ggcacgaacc ggtatcgccg cgtcactggt
     6961 gtggggtggc gttcattatt ggtggcattg tgatcctcgg gagtacggtt taatgggcct
     7021 gatgtgggga ttattcagcg tcattattgc ctcagttgcg caattaagcc tgggttttgc
     7081 ggcgagtcat ctgccgccga tgacgcactt atgggatttt atcgcgacgc tgttagcttt
     7141 tgggctggat gcccggatac tgttactcgg gctgctgggt tatctgcttt ccgttttttg
     7201 ttggtataaa acgctacata aacttgcgct aagcaaagcc tatgccttgc tcagtatgag
     7261 ttatgtgctg gtatggattg cttcaatggt acttcccggc tgggaaggga ctttttcgct
     7321 gaaagcacta ctgggagtag cctgtattat gagcgggttg atgctgattt tcctgcccac
     7381 gacaaaacaa cgttactgag ttttccctgc cactttacac tgccgttccc actcatcggg
     7441 gctataacag cttgcagaga ggactttcac cgtatgcagc ttttcacgat taatacaata
     7501 aagcgcattc tgcaaaggcg agagtaaatc ccccactttc acggcgaagt cactcttcac
     7561 ttcggcgatc atttttatcg cgccgccagc atcgcagagc ataagtacat gtctgttatc
     7621 ttgtttattg cagcacgatt ttttgaccag ccattccatt gtattatcct gtttgctaag
     7681 agttttcata tcaatttaca gattaacaga taaactcttt atcaacggca atggaatggc
     7741 agaactgaga agtgaaacgg agggttaatt attgctgacg ttgcacccac tcttttagcg
     7801 cctgacgtga aattttaata ccgccgtttt tcagctccgg cggcaaagtc agccaacgta
     7861 ccggttgctg aaaacgggcc agcttatctt tcacccattc gtcaagatca acggtttgct
     7921 ggtcatactc caccaccgct accggccgat gaccaaactc cttgtcggca acgggaacga
     7981 taaacacctg caaaaccgca ggatgtgcag caattacgcg ctcgacttct tccggctgaa
     8041 taccctctcc gccactgaag aatagattgt ctaaacgtcc gacaatggtc agcttgccat
     8101 tatgcatctc accgcgatcg cgcgtagcgt accagccttc gtcattaacc agtgaaacca
     8161 gttgcccgtt acgccagtaa ccttctgcca tactggcagc ccgcagccac acttcattat
     8221 taacgatttt cacttcccga cccggcagcg gcgaaccaac gtctgccagg ccgtcggctt
     8281 ctttcgcaca caccgtggag gcaaactcgg tcagaccata gccgcaaaag caacgaatcc
     8341 cctgctcgcg cgcctgttcc gtcaactcga tcgggatagc cgcgccgcca agtaacaccg
     8401 ctttcaggga aacggaactg cggttaacca gcaaacgcca gagttgtgtt ggcaccagtg
     8461 aagcgtgagt acagcctgcc agcatttgct ccaatggctg tttatcacgt accgtcatcc
     8521 gcgcaccagc gtataaccag cgccacataa ttccctgacc ggagacgtga aacagcggta
     8581 aagagagcaa ccagtcatcg tgatcgccaa acggaatcag cgataacaca ccttgcgcac
     8641 tggcaagatg ggcctgataa gtatggacag cggctttcgg caaaccggta gaacccgagg
     8701 tcaacgtcat tgagcacaga cgcgtcggct gccacgcagc ggcatgtgcg ccttcaaccc
     8761 gctgaatgtg cagcgacgtt aatgccggaa acgtgttttc cccatccggc accagcgcaa
     8821 attgcagcgt cagattgggc agcaattctt caagcaacgg ttgcggcagc tgagggttca
     8881 cgggcaacac ccgcgccccg cattgcagta acgccagcca ggcgagcagc gtttgcggcg
     8941 tattccacgc ccgcaacatc acgccgctgc cctcaaccac accctgcacc gcaaatccgg
     9001 aggctaattc atcgacgcga gcacaaagct cgcgccagtt gagttgctcg tcattaagac
     9061 gtaaggcgat ggcttctccc cgcacttgcc gccagtgacg ccacggccag tcagagaaga
     9121 tcataacaac cgctccagtg catcaacttc cacgacaggc agcgtgctac ccggccagcg
     9181 acgtacctgc tgcgcctgca tcagatccag cgtgtccagc ccaggaatgg tgtccggcgt
     9241 taaccaggcg gcaatccgcg ccagttgcgt taagcctaag ctcgattcaa tggaagaact
     9301 gatcaccgcc gtcagcccca gcgcgtgcgc cgcctgtacc tgctcgcgta ctttttccag
     9361 actgcccgtg agcgtgggtt tgataactac cgcgcgcacg ccctcttcag ccacaaaggc
     9421 aaaatccggc tcgcgcaggc tttcatccca ggcaatggca atgccggttt cacgggcaaa
     9481 cgctcgcgaa tcatcgcggg ttttgcacgg ctcttcgaga aacgcgatgc ggtggcgata
     9541 atccgggtta acatatttgg caaactgctg gcctttcagc ggtgtccagg cgcgattcgc
     9601 gtcaagacgc aaatgcagat ccggaattgc ctccagcaac agattcacca ccatgccgtc
     9661 gcgcacggct tcgtacaatc ccactttgac cttcgccact ttctcgcctg gcatatctgc
     9721 aagtttgagg atcagatcgt ccggatcgcc attacacagt ggtgccgtac ggtaattggc
     9781 cgcctgcggg agcgtatctg ccagctccgc caatgcacaa cttacgccaa aggcaacgga
     9841 aggcatctgc ggtaattcgc aatcgcctgc cagccagtta tttacccagg caagcagcac
     9901 actttgcgcc tcttcccagg tttcctgact gaagcccggc agtggggaga tctcccccca
     9961 cccttcgcgc tcgccttcac gcaggcaaac atacagcccg tcgcgggttt ttaaccgcct
    10021 gtcgcgcaga accacccccg cgtccatggg gatctgccag cggtatacct gcgcgctacg
    10081 cattacggat tccgtttaaa tttgctgaaa tcaggctgac gtttctggtt gaaagcgttg
    10141 cgaccttcct gaccttcttc cgtcatgtag aacagcatgg tggcgttgcc cgccagctcc
    10201 tgcagccccg cctgcccgtc acagtcggcg ttcagcgcag ctttcaggca gcgcagcgcc
    10261 atcgggctgt tttgcagcat ttcgcggcac caacggacgg tttctttttc cagatccgcc
    10321 agcggtacca cggtgttcac aaggcccata tccagcgcct gttttgcgtc gtactgacgg
    10381 cacaggaacc agatttcacg cgcttttttc tgcccgacga tgcgagccat gtaggaagcg
    10441 ccccagccgc cgtcgaagga accgactttc gggccagtct gaccgaagat ggcattatct
    10501 gccgcgatag tcaggtcgca catcatgtgc agaacgtgac cgccgccgat ggagtagcca
    10561 gccaccatcg cgacaaccgg tttcggacag gtacggatct gacgctggaa gtccagcacg
    10621 ttcaggtgat gtacgccgga atcatcttta tagccgccgt aatcaccacg tactttctgg
    10681 tcaccaccgg agcagaacgc tttatcgcct gcaccagtca gaatgatcac gccgatgttg
    10741 tcgtcataac gcgcatctgc cagtgcctgg atcatctctt ttaccgtcag aggacggaag
    10801 gcattgcgca cctgcggacg attaatggtg atttttgcga taccgtcggt ggatttttca
    10861 taacgaatgt cctcgaaacc ttcggagcag tcgtgccatt caaccggtgc gtaaagcatt
    10921 gcttcatcag gataaatcat attgtgtcct ttagtcagaa acgcaagatc tgcgccagac
    10981 ttgcgataac gccagcggga ttttcccgat gcgcgttatg tccggcgcga ggaatgacat
    11041 ggcagtcggc agccagttcc gccgccagag cgcggaattt gctgtcacgt tcaccacata
    11101 aataataaaa cgcaaatgtg cgggcgctaa ggttagcacg taaatcaggc tggacggcga
    11161 gagaagtcgc ctccagcatg gcggcaaggg ttgcgccatt attattgctg cgcagcgcca
    11221 ccagctcccg gcgttgatcg tcattgagtg aggcaaaaac aggctgttga taccagtcgg
    11281 caaataccgc cgttaacggt tctgtgcgaa aacgctgcac ccattggcga tcggaacgct
    11341 gacgttccgc acgttgttca gcattttgca gccccggatg cccgccttcg acaataaccc
    11401 cataaagccc cgccagcccc tggcaagccg ccatcatcgc cacccgtccg ccaagcgagt
    11461 accccaccag ccagaagtca aggatgttgt aactaaccaa agttttacgc agtaagtcag
    11521 tgacatcatc aaatccatcg acgctaatcg ccgccgaacc accgtgacct gggagatcaa
    11581 catacaaccg tgagtagtcg gcaaacacct cgcccacttc ttgccattcg tggcaatcgc
    11641 cggaaaaacc gtgcaaaaaa accagccagg gtaaacctgg tttgccgtgt tttgcctgcg
    11701 cgtgcaggat cataaatggc ttacctgcgc cagcagttgc tggagcgttt gcgcgccgtc
    11761 ggtgtcgtta accaccattt caattaccgt ggtggtcgga gtacgccagg cgtcggcaag
    11821 taccgtttca agttcctgcc agttttgcgg acgatgatat ttcagctcaa acatcgcggc
    11881 agcgtgctca aaatggacgt tttgcggcat cagataaaaa cgctcgcgct cgcttttcgg
    11941 cgtcggcagc agcgagaaaa tttgcccgcc gttgttgttc accacaatta ataccagcgg
    12001 cgcggaaacc tggcgcaata acgccagcgc attcaggtcg taaagtgcgg agagatcgcc
    12061 cacaatcgcc agcgtcggtt tgccgcttgc ccgctgaacg ccggcggcag tcgagagcag
    12121 cccgtcgata ccgctggccc cacggttgct gtacaccggg taacctgccg gaagttgcga
    12181 aagcgcatca atcagacgta ccaccaggct gttaccgaca aacaattgcc cctgttcagg
    12241 cagatagtcg cagacgcgat gcgccagttg cgcttcgcca aacgcatcgc gacgggcaat
    12301 aaccgcctgc atcgcctgct ccgccaggcg cgggatttca acgcaccagg gctggcgttt
    12361 ttctgccgga tgcagttcca gccagtcggc aatattggca attaaccgac gcccgcgatg
    12421 gtgcgccgga tcaagccgcc cttcgatgtc atccacaatc cagtactctt cgggttcaca
    12481 gcttgcctgc cattgcagga gccgtttgcc agtcaggctg cttcccagtt gcaccacaat
    12541 ttgcgcctgt tgcagttcgc tggtcgcttt ggcattgcct aaccagagat cggcacacgg
    12601 cagcggctgt ccggtttgcg acagcacgtc gccaatcagc ggccagccaa gagtttgcgc
    12661 ccacagtgca acttttttgc cctcttccgc actcatgcgc ccggcaacca ccacgccgcg
    12721 cttttgccgc cagaagaacc agtcgcgctg tttttcgctc tcaagacgag gcgcttcacg
    12781 cagccacggc ttatcatcct gccaccagtc acccagaagc tgttgccagc taagcccagt
    12841 gtcgtccatc tcgccataca gcggttcagc aaacgggcag ttgatatgga ccccaccagc
    12901 atgaagcgta ccgagagcgt ggtcgatggt agaaaccagc caacgtgcgg ggatatcctg
    12961 ggtcgggcgc ggcagtgaaa tactgtgcgt ggggtgagag gcgaacattc ccggctgacg
    13021 aatcgcctga ttcgcgccgc agtcaattag ctccggcggg cgatcggcgg ttaagagaat
    13081 cagtttttct ccggttaacc cggcttcaat cagtgccgga tagagatttg ccaccgccgt
    13141 accggaggtc acaatcaccg ccaccggctg cttgctgact ttcgccagcc ccagcgccag
    13201 atgccccagt ccacgctcat cgaaatgggt gtggtgaatg aatgcggaat tctccgccgc
    13261 cgctaacgtt aacggtgtag aacgcgagcc aggggcgata cagatgtgtc ttacgccgtg
    13321 acgcgttaat gcttccagaa tgaccgccgc ccagcgtcgg ttaaatgcgc ttactgacat
    13381 gagtttgtcc ggtatcaata ttgcggctaa gtataaggag ctacaaaaat agattattga
    13441 tatgaatcgg taatgatgcg actcattact attccatttg taataaagta cgcagccctg
    13501 ccgctttgtt gtcgatttcc tgccactctt gctcggggtc ggagccacgg acaatgcccg
    13561 cgccagcata tagccgcacg acattgccgc tgatttttgc tgagcgtagg gaaacgcaga
    13621 attcgctttg ctgtaatgag agatagcccg cagaaccggc gtaccattct cgggtgaacg
    13681 gttcgtgacg ggcgataaac tgtcgcgcca gatcgcgcgg taagccagca actgctgccg
    13741 ttggctgcaa ctgatgtaaa cagatcacat catccgcttt gttgagtaaa gtccagatac
    13801 agcggcgaag atgctgcact ttacgcagac gcagtacctg cggcggtaaa acatccagcg
    13861 tctgggtatc ggcctgtaat cgttggcaga tatcttccac caccagcatg ttctcgcgct
    13921 ggtttttatc atccgccatc agccactctc ctaactgctg cgcctgctta tcatcaggat
    13981 gatttgctac tgttcccgcc agcgcttcag tacgcagcgc tttgtcacgc cgtcgccata
    14041 accgttccgg tgaagagcca agaaaagcat tttcgccatc aaaggccatg taaaaatggt
    14101 agcaattgag attcagtcga cgactggcag ccatcatcgc cgccgcattg accggacttg
    14161 cgaaatgcag gtcagttgcc cgagcaagca ccactttgtc gagctcacct tcggcgatgg
    14221 ttttcgttgc cagttcgatt aattgcgtcc agcccgtttt gtccggccag tgttgttctc
    14281 gcgtagtggt taaatgtaac ccaggcaagg gcttgatact caccagtgtg gcgataaatt
    14341 ctcttgcctt aatcgcatcg tgctgaaggg agctttcgct gaatagcgtc agccgcagcg
    14401 tggctttacc gccacagcgt cgccattcca ggcgtggtaa aagtaaattg ccctgcgacg
    14461 ggtcaaacgc attcagcccc caaatgcgta agtcggcgtg ttccgggtgc tggcgaagaa
    14521 aacgttgtgc ctggtccaac gacgtaaaac gggtaatcgc gcccaggacg gcagcttctt
    14581 catcaccatt acgttgttgc cagtagaatt gcgggtacgt ttgctgactg gccagccagc
    14641 tcaaggcatc aaaagcgtct ttgagaggga aaggaatatc gataacccga atgccgggtg
    14701 tcgccggaat ctcttgcgac aaatggcgca gtagattttc cagcgccgta gtaagtgatt
    14761 gcacgcggac ctctccctgt taaaagcctc acattatacg gggtactaca aaaaaaatgc
    14821 agtaccccgg tgtagggagg ttttaacggc gtgccagcaa cagtcctagt accagcccaa
    14881 cggccgcacc cacaccaatt ccctgccagg gtttttcgtg gacgtagtca tcagcacgat
    14941 aaacagcctg cttcgcccga tagtaataac tgtctgaagc ctggctaacc cgttttttca
    15001 catcatccag cgctttttct gcacgcgctt tcagctcaac atatttctga tcggcgggat
    15061 cgccagagga gcggagcacc tcttccagtg tttcactaag cagcgtcagg tcgtcatcga
    15121 tacgtgtatc accaaactga ttagacattc tcgttctcca tttgcgtaaa acctgtcact
    15181 aactatagac aagggttatt gattacgcct gaattacctc gcgtgccatc ccaatgtgtg
    15241 ggataccatc ttcttcatag atctccgtca cagggataaa accgaaactc tggtagaagt
    15301 tttgcagatg cgcctgcgcc ccaaggtaaa caggcttatc aggccagtga tgcgtgcatg
    15361 tgtcgagggt tttactcatc agttgctgcc ccactttttc gccacgcaac gcttcactga
    15421 caatcacccg acctataacg accggctcaa gattatcatc acttttcaga atcctcgcat
    15481 acgccaccag ttcatcattt ttccaaccga gaatatggcg attatccccc gtcaaatcat
    15541 caccatcgat atcctgataa gggcaattct gctcgacaac aaacaccgcg caacgcagtt
    15601 gtaataaggc ataaagctgg gaaaccgata attcagagtg atgcagatct tgccattcaa
    15661 tcataatgct ctccttttag atgtatgcct tatactagcc ggaatatttt ttgaaacgtg
    15721 atgaactcat ggaattaatt tttttaggta cttcggccgg tgtgccaacc cgcacgcgca
    15781 atgtcacggc aatattgctt aacctgcaac atccgaccca gagcggtctt tggttgtttg
    15841 actgcggtga aggcacccag catcagctac tgcataccgc ctttaaccct ggaaaactgg
    15901 acaagatttt tatcagtcac cttcatggcg atcatctttt tggtttaccc ggcttgctgt
    15961 gcagtcgttc tatgtcaggc attatccaac ccttaacgat ttatggtccg caaggtatcc
    16021 gtgaatttgt ggagaccgcg ctgcggatta gcggctcatg gaccgattat ccgctggaaa
    16081 ttgtcgaaat tggcgctggc gaaattctcg acgatggcct gcgcaaagtg accgcttatc
    16141 caatggaaca cccactggaa tgttatggct atcgtattga agaacatgat aaaccgggtg
    16201 cattaaatgc ccaggcatta aaagctgctg gcgtgccgcc tggcccactg tttcaggaat
    16261 taaaagcggg caaaacaatc acgctggaag atggaaggca gattaacggc gcagattact
    16321 tagctgctcc agtgccaggt aaagcgctcg ctattttcgg cgataccggc ccctgcgatg
    16381 ccgcacttga cctggctaaa ggtgtcgatg tcatggtgca cgaagcgacg ctggatataa
    16441 ccatggaagc caaagccaat agtcgcggcc atagctctac acgccaggct gcgacactgg
    16501 cccgtgaggc tggagtcggt aagctaatca ttacccacgt cagctcgcgc tatgatgaca
    16561 aaggttgtca gcacctgtta cgtgaatgca ggtcaatttt cccggcgact gaactagcga
    16621 atgatttcac cgtgtttaac gtttaactct gcttattatt aaaaacaggg cgaaacttgc
    16681 cctgttatcg caacccgcgc tacatatatc cactcacacg ggcatattga gcgatttttg
    16741 atatttattc atctccttac gcactatatt cagagcaata agttgcctct ttttatcttc
    16801 attcagcaca acctttgcaa ctatttgcaa ctaaaaggag catatgatgg ttacagttgt
    16861 cagcaattat tgtcaattat ctcaaaccca actcagtcag acatttgcag aaaaatttac
    16921 tgtgaccgat gaattactgc agtctttaaa aaaaacagcg ttatccggag atgaagaaag
    16981 tattgagtta ctgcataata ttgcgttagg ttatgatgaa tttggaaaaa aagctgaaaa
    17041 tattctttac catattgtta gaaacccaac aaatgatacc ctatcgatta tcaagcttat
    17101 aaaaaatgcc tgtttaaaat tatataattt ggcacatacc gcaaccaatt cccaccttaa
    17161 accaactgct ccggataatt cagatgatct cctgttcaaa aagctatcct ccccttcgaa
    17221 attaatgaca attatcggcg atgaaattcc tcttatatct gaaaaacagt cgctttcgaa
    17281 ggtactttta aatgatgaga ataatgaact gagtgatggt acaaacttct gggataaaaa
    17341 tcgtcaatta accacagatg aaatagattg ctatcttcag aagatcgccg ctaatgcaaa
    17401 aaatactcaa gtcaattatc ctactggtct ctacgtcccc tactccacca gaactcatct
    17461 ggaagacgct ctcaatgata atattaagag cgatccatca tggccgaaag aagtccagtt
    17521 attccccata aatactggcg gacactggat attagtttcg ctacagaaaa tagtaaatga
    17581 aaaaaataat acacaacaaa ttaaatgcgt ca
//
LOCUS       DAPGOF010000099        17490 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.100, whole genome shotgun sequence.
ACCESSION   DAPGOF010000099 DAPGOF010000000
VERSION     DAPGOF010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.100"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            97..813
                     /gene="nanC"
                     /locus_tag="RHV13_002924"
     CDS             97..813
                     /gene="nanC"
                     /locus_tag="RHV13_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HDV1242439.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVDVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            833..1939
                     /gene="nanM"
                     /locus_tag="RHV13_002925"
     CDS             833..1939
                     /gene="nanM"
                     /locus_tag="RHV13_002925"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HDV1242440.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWKALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2004..2984
                     /locus_tag="RHV13_002926"
     CDS             2004..2984
                     /locus_tag="RHV13_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HDV1242441.1"
                     /translation="MNAIISPDYYYILTIAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPPCHFNDMIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPHNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEVIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDKFVEAILQFWRER"
     gene            complement(2992..3642)
                     /locus_tag="RHV13_002927"
     CDS             complement(2992..3642)
                     /locus_tag="RHV13_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDV1242442.1"
                     /translation="MNKLPDHLPRRYIIEAIHLWDAGETHQFQPARLYEIEYHGRRYP
                     SKAIAGIAATLMTGTQFTPADFTGGIKSKCVKLLIEQGFHIHLDKAAAAPIADVLFPD
                     ELPSQETYLEGAAIKVTVNRYERDKKARDKAVEHHGCQCNVCGVDLVKIYGDIAEGFI
                     HVHHLVPLSAIKEDYQLDPVNDLLPVYPNCHAMLHRRKPPFTPEQLKALMDANKSN"
     gene            complement(4703..4855)
                     /locus_tag="RHV13_002928"
     CDS             complement(4703..4855)
                     /locus_tag="RHV13_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242443.1"
                     /translation="MSDITAYRPEVVNENTDVICSTSIRRILAVRKSSLLQIDTIIRH
                     QVSDIS"
     gene            complement(4940..5137)
                     /locus_tag="RHV13_002929"
     CDS             complement(4940..5137)
                     /locus_tag="RHV13_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242444.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNNEDKTDSAALLPC
                     IEQAREDIRTLRQLQLQHQNR"
     gene            complement(5149..5637)
                     /locus_tag="RHV13_002930"
     CDS             complement(5149..5637)
                     /locus_tag="RHV13_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242445.1"
                     /translation="MKLSLMLEADRINVQALNMGRIVVDVDGVNLTELINKVAENGYS
                     LRVVEESDQQSTCTLPPFATLAGIRCSTAHITEKDNAWLYSLSHQTSDVGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVITLTRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            complement(5634..6008)
                     /locus_tag="RHV13_002931"
     CDS             complement(5634..6008)
                     /locus_tag="RHV13_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HDV1242446.1"
                     /translation="MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPEKR"
     gene            complement(6098..6466)
                     /locus_tag="RHV13_002932"
     CDS             complement(6098..6466)
                     /locus_tag="RHV13_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HDV1242447.1"
                     /translation="MSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLGSGGYVYLAVYPTPETKK"
     gene            complement(6629..6850)
                     /locus_tag="RHV13_002933"
     CDS             complement(6629..6850)
                     /locus_tag="RHV13_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HDV1242448.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(6913..7389)
                     /locus_tag="RHV13_002934"
     CDS             complement(6913..7389)
                     /locus_tag="RHV13_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HDV1242449.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(7405..7890)
                     /locus_tag="RHV13_002935"
     CDS             complement(7405..7890)
                     /locus_tag="RHV13_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDV1242450.1"
                     /translation="MKTISHNSTTSSVSVTAASGNDQPQLVATLVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(7945..8763)
                     /locus_tag="RHV13_002936"
     CDS             complement(7945..8763)
                     /locus_tag="RHV13_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HDV1242451.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPGIFGEDKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLILPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESLR"
     gene            complement(8863..9096)
                     /locus_tag="RHV13_002937"
     CDS             complement(8863..9096)
                     /locus_tag="RHV13_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HDV1242452.1"
                     /translation="MPGYTEYVLAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRVIR
                     NAEGQRRWRCRNSEPDAGKVLNTRLASDGLLRQ"
     gene            complement(9175..9630)
                     /locus_tag="RHV13_002938"
     CDS             complement(9175..9630)
                     /locus_tag="RHV13_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242453.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFNPQDYHPLLITVSGKNVN"
     gene            complement(9706..12222)
                     /locus_tag="RHV13_002939"
     CDS             complement(9706..12222)
                     /locus_tag="RHV13_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242454.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALISTASALAGLILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAGGLLAVPLQIKNIQQNGITPEDISKEINGYYCCFYTAFFLMACSACAPLIALQYD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKKLTCAISQTLEEQPVLNSKSWLTSLQ
                     NDYSLPDSLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRVCA
                     SCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQEIYERPYNSFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNNGPMQLEVPRDQYAGAVETMRNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDAALQTAAVQAGKTFTRTLAVYLTTQQLHRLSVVQGMLKHIDFSTASPT
                     VRLALQKGTGAGNISALNKVMKGTLVTSLALVAVTTGPDMIKILRGRISGTQFIRNLA
                     VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNENLARVIDQNALEIIFAAGIQQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHLGNMVNRLEEALPPELPA"
     gene            complement(12343..15462)
                     /gene="ag43"
                     /locus_tag="RHV13_002940"
     CDS             complement(12343..15462)
                     /gene="ag43"
                     /locus_tag="RHV13_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HDV1242455.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADKVVQAGETVNDGTLTNHDNQIVLGTANGMTISTGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATGTVINEKGWQAIKSGAVATDTVVNTGAEGGPDAENGDTGQTVYGDAVRTTINK
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASGTVVNSDGWQ
                     IVKNGGVAGNTTVNQKGRLQVDAGGTATNVTLKQGGALVTSTAATVTGINRLGAFSVV
                     EGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADSG
                     AAVSGTRSDGKAFSIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLAG
                     TTTLNNGAILTLSGKTVNNDTLTIREGDALLQGGSLTGNGSVEKSGSGTLTVSNTTLT
                     QKAVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNIP
                     DNATVQSVVDDLSHAGQIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQNNA
                     DRLVIDGGRATGKTILNLVNAGNSASGLATSGKGIQVVEAINGATTEEGAFIQGNKLQ
                     AGAFNYSLNRDSDESWYLRSENAYRAEVPLYASMLTQAMDYDRILAGSRSHQTGVSGE
                     NNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSVTAGVYG
                     AAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLIHNASGLWADIVAQGTRHSMKASSD
                     NNDFRVRGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNASYVKFGHGS
                     AQHVRAGFRLGSHHDMNFGKGTSSRDTLRGSAKHSVRELPVNWWVQPSVIRTFSSRGD
                     MSMGTAAAGSNMTFSPSQNGTSLDLQAGLEARVRENITLGVQAGYVHSVSGSSAEGYN
                     GQATLNVTF"
     gene            complement(15834..16706)
                     /locus_tag="RHV13_002941"
     CDS             complement(15834..16706)
                     /locus_tag="RHV13_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HDV1242456.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRAQAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
BASE COUNT         4230 a         4404 c         4516 g         4340 t
ORIGIN      
        1 actataagcc tggcaaaaaa atattacaca acataaatgc taattgttta tgcgggcttt
       61 gtattgcttt ctgtttccta caaatgagtg aaatttatga aaaaggctaa aatactttct
      121 ggcgtattat tactgtgctt ttcgtcacca ttaatttctc aagctgcgac actggacgta
      181 cgtggtggat atcgtagtgg aagccacgcc tatgagactc gactcaaagt cagtgaggga
      241 tggcaaaatg gatggtgggc aagtatggaa agtaatacct ggaataccat tcatgataat
      301 aaaaaggaaa atgccgcact caatgatgtt caggttgatg ttaattacgc gattaaactt
      361 gatgatcaat ggacggtgcg cccgggaatg ttaacgcatt ttagcagcaa cggcacacgc
      421 tacggaccct acgtaaaact gtcctgggac gcgacaaaag atcttaattt tggcattcgc
      481 tatcgttacg actggaaagc ttaccgacaa caagacttat ccggtgatat gtctcgtgat
      541 aacgttcatc gttgggatgg atatgtcact taccatatta atagtgattt caccttcgca
      601 tggcaaacga cgctatacag caaacagaac gattatcgct atgcaaacca taagaaatgg
      661 gcgacggaaa atgcatttgt tctacaatac catatgacgc cggatattac gccatacata
      721 gaatatgact accttgaccg tcagggtgtt tacaacggca gagataattt atcggaaaac
      781 agttatcgca ttggtgtgtc atttaaactg tagtagacag gagacagtca caatgaataa
      841 aacaataacg gcgcttgcta tcatgatggc ttcatttgcc gcaaacgcgt ctgtattacc
      901 ggaaactcct gtgccattta aaagtggtac cggagcaatt gataacgaca ctgtctacat
      961 tggtttaggt agcgcaggta cggcatggta caagctggat acacaggcca aagataaaaa
     1021 atggaaagcg ttagctgcat tccctggtgg accaagagat caagcaacct cggcatttat
     1081 tgatggcaat ctgtatgtgt ttggcggcat tggcaaaaac agcgagggct tgactcaggt
     1141 atttaatgac gtacacaaat acaaccccaa aaccaatagt tgggttaaat tgatgtcgca
     1201 cgcaccgatg ggcatggcgg gtcatgtaac ttttgtacac aacggcaagg cttatgttac
     1261 tggcggtgtt aaccagaata tcttcaatgg ctattttgaa gatctcaacg aggctggaaa
     1321 agattcaacc gctatagata aaatcaacgc tcactatttt gacaaaaaag cagaagatta
     1381 ttttttcaat aagtttctgt tgtcttttga tccctcaaca cagcaatgga gttacgctgg
     1441 cgaatcgccc tggtacggaa cggctggtgc ggcggttgtg aataaaggtg ataaaacctg
     1501 gcttattaat ggcgaagcca aaccaggatt gcgaacggat gccgtatttg aacttgattt
     1561 caccggtaat aatttaaaat ggaataagct tgctcccgtc tcatcaccag atggcgtagc
     1621 tggcggtttt gcggggataa gcaatgattc tcttatattt gccggagggg ccggattcaa
     1681 aggttcacga gaaaattacc agaacggtaa gaactatgcg catgaaggcc tgaaaaaatc
     1741 atatagcact gatattcatc tttggcataa cgggaaatgg gataaatcgg gtgaattatc
     1801 gcaaggtcgg gcctacggag tatcattgcc ctggaataat agtctattga ttattggcgg
     1861 tgaaactgca ggcggcaaag cggtgacgga ttcagttttg atctctgtga aggataataa
     1921 agttacagta caaaactaac gcttcagggc cccggtaaag gggccctgct atcaatttgt
     1981 catttcaatt acgcgaaatt tatatgaacg caataatatc gcccgattat tactatattc
     2041 ttaccattgc tggtcagtct aatgccatgg cgtatggcga aggactgcca ttaccggaca
     2101 gggaagatgc gcctcatccc agaatcaaac aattagcgag atttgcgcat acgcatcccg
     2161 gaggcccgcc atgtcacttt aacgacatga ttccactgac tcactgccca cacgatgttc
     2221 aggatatgca gggttatcac catcctctgg caacgaatca tcaaacacag tacggcaccg
     2281 ttggccaggc actgcatata gcacggaaat tactgccctt tattcctgat aatgcagggg
     2341 ttctcatcgt tccgtgttgc cgtggcggat cggcttttac cgcgggcagc gaagggacat
     2401 attcagaacg gcacggagcc agccatgatg cttgtcgttg gggaacggat actccgctat
     2461 accaggattt agtcagcaga acgcgagccg cactggcaaa aaatccgcat aacaaattcc
     2521 tcggcgtatg ctggatgcaa ggcgaatttg acttaatgac cagtgactac gcgtcacacc
     2581 ctcaacactt taatcatatg gttgaagcct ttcgtaggga tctaaaacaa taccattctc
     2641 agcttaataa tattactgac gcaccgtggt tttgcggcga taccacctgg tactggaaag
     2701 aaaatttccc tcatgcgtat gaagttattt atggcaatta tcaaaataat gttttagcca
     2761 atattatttt cgtcgacttc cagcaacaag gtgaaagagg actgacgaac gcgcctgatg
     2821 aagacccgga cgatttaagc acgggatatt acggttcagc gtaccggtca ccggagaact
     2881 ggacgacggc actgcgaagc agtcatttca gcacggcagc ccgtcggggg attatttctg
     2941 acaagtttgt agaagcaatt ttgcagtttt ggcgcgaaag gtgagcgttc attaatttga
     3001 tttattcgca tccatcagcg cttttaactg ctccggcgta aacggtggtt tgcgtcggtg
     3061 caacatcgca tggcaattag ggtacacagg cagcaaatca ttaactggat ccagctggta
     3121 atcctcttta atggctgaca gcggcaccag gtgatgcaca tggataaagc cttcagcgat
     3181 atcaccatag atcttcacca gatcaacacc gcatacgttg cactggcatc catgatgttc
     3241 gaccgcttta tctcgggcct ttttatcgcg ttcatagcgg tttactgtca cctttatcgc
     3301 cgcaccttca aggtatgtct cctgcgacgg caactcatcc gggaacagaa cgtcggctat
     3361 gggagccgca gcggctttat ccagatgaat atgaaatccc tgctctatga gtagcttgac
     3421 acatttagat tttattccgc cggtgaaatc cgcaggggta aattgcgtcc ccgtcatcaa
     3481 tgtcgctgcg atgccagcaa tcgccttcga ggggtaacgt cggccatgat attcaatctc
     3541 atacaaccgc gcaggctgga actgatgagt ttctcccgca tcccataaat ggatagcttc
     3601 gatgatatac cgccgcggaa gatgatcagg gagtttattc atggagaatg tccttatacg
     3661 ttctcagaac aggcagcaaa aaaacaaatg agatcaaggt aatatatctt cacttgcagg
     3721 cttgaacagc agaaaatttg cgagcatgat cgcaattatt tcactgccag ggatatcaat
     3781 agcttccagt aaatcgctac agcaggcaat tgccaatata aaaatctggc acaagggtga
     3841 acagcgcgcg ccgcataagc cattgttatt gctatacgta ttagcgggat acctgaatgg
     3901 acatccgcgc cttttcgatt attgtagtgg aacagtctac gaggatgcag ttcgaagtga
     3961 gtaagtaaag gttgctggaa acgatcaatg ccgccataca atgatttttt aggatcaaca
     4021 atgagtaata aaaactacga aagtcaccgc aaagcgattg ttagtaaagg tataccaccc
     4081 gctctattaa ataggctcac caattcggat gttcaggtga tcaatacctt cttaacacga
     4141 gtgagcaaac tggaactatc tcaacaagag aaagactgga tcataaaaat catctctatg
     4201 gtttagacaa tttcagcctt gtagcctttc aagggttttg tgtgggtaca aacattatcg
     4261 aatcggggta tagccgcagc tgtagctgta tccacatcag taagttgatg taggttgact
     4321 taggttgagc gaaaaatagc gaaaagcctt gtgtggcgcg gatttcagtc ataaaaaaac
     4381 gtccgttgat gtctattgat gttccgatgg tgcgaaggtg cgaaggtgcg aaggtgcgaa
     4441 ggtgcgaagg ccggactcaa acatgtgact taacctcatg atttaaaatt gttaataata
     4501 aacaatatca tcttatacca acacagatac caacacgaaa aatcactggt tttcggcatc
     4561 gaaccaggac aatcagctcc tgattgagat accgagctct gttactcgtc atcttcctcg
     4621 acaggcttca ttgcatcaag tagtactcgc accatcgcga tagaaccaat ccggataaaa
     4681 gcatcttctc catctatatc tgttaactga tatcgctcac ttgatgccgg ataattgtgt
     4741 cgatttgcag cagtgaactc tttcgtacag ccagaatgcg gcggatgctg gttgagcaga
     4801 taacgtccgt attctcgttg accacttccg gacggtaggc tgtgatatca gacataatcg
     4861 atattccttt ttgcagataa atgaaaatgc cagccctgtt caggctggcg tcagtggaat
     4921 gaagtctggt gagtaagact caccggttct ggtgctgaag ctgcagttgg cgcagggtac
     4981 ggatatcctc tctggcctgt tcgatacagg gcaacagtgc tgctgaatcc gttttatcct
     5041 cattgttgtc aaagataatt cccgattcgc agtcgatatt gtcctgcagc cacgcaatca
     5101 gaatatccag tgcggtttcc gtggttaatg atttcatgtt gttcctggtt accagttgaa
     5161 agtgggtaaa cccggcaggc attcagcgct ggcatccaga tgaatgagac tgacgccata
     5221 acgtcgggta agtgtgataa ccagacgacg gaacgttttt gacagcccca ggcgtttaag
     5281 ccgcagaacc ggatatgacc acgcatcggt acgtaacaga tatccgctac ctgtgaaatg
     5341 aatccattct gattcaccaa cgtcactggt ctgatgcgac agcgagtaca gccaggcgtt
     5401 atccttttcc gtgatatgtg cggtactgca gcgtatgccg gcaagggttg caaacggtgg
     5461 tagtgtgcag gttgactgtt ggtcggattc ctccaccacg cggagtgaat aaccgttttc
     5521 agcgaccttg ttaatcagtt cagtgagatt aacaccatcg acgtcaacga caattcgccc
     5581 catgttcagt gcctgcacat taattctgtc ggcttccagc atcagggaaa gtttcatcgt
     5641 ttctccggat gcttacccag ggtaatgtta tttaccgttc tgtaattgtc gcgggtcatc
     5701 aggccggtcg ccctgcgagc ccggaggata tcaatactgt ttattaactg agagcgggga
     5761 caagtgctga atcccggctg gtcggtacgc accagcgcgt atttttccac gagaaaattc
     5821 accgcatcac acagtgaaat gccagcttca atatgctgct caatcacacg ttcatcggcg
     5881 aacggtgtgt cattcagtgt aaggccgtaa tgctggccca gtagtcggga gagcagagtc
     5941 tgccagatgg tgacgggaga cgggcagctc gatgcctccc gtacgtgtgt atcgggtaat
     6001 gttttcatac tgaaggtttt cctgatatgc agagatgaaa aatggaaaag tggcgtggtg
     6061 aaagcaccag gccggagcag aaggttattc cggtggttta ctttttcgtt tcgggcgtcg
     6121 gataaacagc cagataaacg taaccaccgc taccgagagt gtcagctttg caggtcagac
     6181 cttttgcata cagcgtgacg gtatgctgat ggcggggatt cagttcaccg ctggtgagca
     6241 tgagttccag ttgtttcatc agcagcggaa aggcctggtc caggtggtac gcatctgcat
     6301 cgctgaacag gcctctgata ccggcgcggt cggcaaggta atgcaggcgg ttaccctcct
     6361 gcaccagacg ggcgccgaaa cagggcgtca ctgtgcaggg tagtccccac cagggatggt
     6421 cgtcattgtc atcgggatgc gttgtcccgg aaagtgtgtc tgacacgata aaatcctcac
     6481 agaaaatcgg tgaatgatag tttaacgatg acgggtcagc cggcgcgcag accggcttcg
     6541 gtaaaggtgt attcgttgat gagcaggacc tcatccacag cggcgtctga gctcagccag
     6601 tcgtactcgt tttccgggtg acggaacatc agttcagggt gacgctcatc agccggacat
     6661 acggaccaaa actgtcctta cggcgttctg caaacacggc cagcacaccg ggaatatcct
     6721 gcacttcacg accggtatac gcttcagcac tgccgtgcca gcggtatttg ccggtacaga
     6781 acggaaaaag acgggatgcc ggatgctgac ggtgaatacg catggcttcg ccacgggtga
     6841 tgattttcat agtgggatac ctctgaagac agaagataaa agtgaaaaca ggtgtgatgt
     6901 ggttgtgacg ggttaaagca gaccatgttc ggcaaaggag aaaacctggc tgccaccgac
     6961 tatcagatgg tccggcaccc ggatatccac caggcccagt gcctgtacca gacgttccgt
     7021 gataaggcgg tctgccttgc tgggtgtgac ttcaccggac gggtgattgt gtgccagcac
     7081 cacagcagcg gcattgtgat acagggcgcg tttaatcact tcacggggat ggacttccgt
     7141 gcggttgatg gtgccggtga agagggtttc accggcaatc agctgattct ggttgttcag
     7201 atacagcacc cggaactctt cacgctccag tcccgccatg ttcagaatca gccattctcg
     7261 tgccgcacgg gtggaggtga aggccacgcc gggttcatga agatggcggt ccagggtttt
     7321 cagggcccgt tgaatgaggc tgcgctcgcc gggcgtcatc tctccgggca gaaaggaaag
     7381 ctgttgcatt gtacttctct ccattcagtc gatgatgcgc ataatggcgc tgcattccgg
     7441 atgctgcagg gcgtaatccc gcagccggta ataatggacc gtcatggcat aacactccgt
     7501 acgacaggca tgatgactgt acgtcatcag acaggcagca atgccggcag cttccggact
     7561 catttcagcg cggttaccgt tcatggcatt gaacagtacc catgtttcgt catcatcgtc
     7621 atccggttcg ggtgccataa atgccccgcc gttgttcagg gtgtacagat tccagatacc
     7681 accgcagtag tcttcacaca gacggtccat ccagccgaag acacggggct ccagggtcac
     7741 ccactgtgga atgaggccaa agtgctgcgg ccagaagctg atgcgctgtt catcagggac
     7801 gagggtggca accaactgag gctggtcatt ccctgatgca gcggttacgg aaacagaaga
     7861 tgtggtggaa ttatgtgaaa tggttttcat gaaatgactc cttaaaggac aaataaatgc
     7921 gtggttttca gggtaatggc tgaatcagcg caggctctcc agcagggttt ccgccatcac
     7981 ccacaatgcg cggttgagct taatgtcagt gtcaatgctg tgaatggcac gggtatggat
     8041 acgttttcct ttagcgctgc gacctgaaat tccgcctttc agcatattct cctgaatggt
     8101 ctgataagca ctccacaggt ccttaccgta atcctcccgg cgtcgtggcg tcagaatgtc
     8161 ggcggtggtg acgggctgat gttcgtcacc ataacggtaa gtcagtgccg cctgtgccag
     8221 cgcctggcgt gccggtggcg gcagaatcag cgactgcatg gcatcacgct tttcctcaat
     8281 ccggtcaaac acgcccacca cctcgtaagc cccttcaatg actctgtcca ccacatttcc
     8341 ccggtgcgga acacgcactt cccccagaga ctgaccacag acgcatccgt tctggcagac
     8401 gaacctgaag taacccggca gcatctggta gctggaggta ccgtcatgag agttgagcag
     8461 aataatttca gggacatgtt ctccgtttat ctctccggcc cgccgcagac gcagcatgtg
     8521 ttttgtgtat ccccggcggc ccgggtcgcg cacacgggtc tggcaggcga agaatggctg
     8581 aaagccttcc cgctgcaggc tttccagtac ggtgatggtg gggatgtacg tatagttctg
     8641 gcttcgggac gtgtgtttat cttccccgaa aatccccggc acatagtgca tcagttcttc
     8701 gtgtgtcagc ggacggtcac ggcgtatctg gttcgcataa ccaaaacgac tggctagtcg
     8761 cataatttgc tccttatcgg tggttaagat ttactggtgt aataaatgaa aaagccacgt
     8821 ctcccggaga agacgcggcc tgatgggtga aatgaatgac gtttattgtc tgagaagacc
     8881 gtcactggcg agccgcgtat taagcacctt tccggcatcg ggttcagaat tccggcagcg
     8941 ccagcgtcgc tgaccttccg cgttgcggat aacccgacgg aaatgcattc ccgggtcatc
     9001 ctggataaac acattgcggt aagcggttat gacagcaacc gcctgcccgt atgagaaaga
     9061 tccttcagcc aggacatact ctgtgtaacc cggcatatct gttttctcct gataaataaa
     9121 aagcctctgc cacgcgttgt gacagaggct gaagcatgaa actgacttca gggatcagtt
     9181 aacgtttttt ccggaaacgg taatcagcag tggatggtag tcctggggat tgaaaaccga
     9241 taacggcaga ctgacacgat ggccgttact ttcttcagtt gctttgatga tttcggttgt
     9301 ggcgacattt tccacgcact ccgtttccag aaatgcgtct gtggttcgcg tggcattact
     9361 gtcaccaaaa gcttccgttt ccatttttct ggtcaccagc gtctgaccat atttgtcttt
     9421 gagttgcaga gtgatggtga gggggccaaa tccttcatcg tttccgccat tatccagccg
     9481 gaactggtaa gcacaaatat ttcccgggag ccatatcgta tctgtattgc gtatactgat
     9541 gtaacgttga tcctgtgccc ggagtggggc agaccacgtt aaccccagaa tgaaggcggt
     9601 aatcatgcag gttttgaaca ggtgaatcat ggtatttacc tctctgagtc atgacgatga
     9661 cactgacaaa tcaggtgata aagcgtaaaa ggcgcagaat agccgttatg ccggtaactc
     9721 cgggggtaat gcttcttcca gtcggttaac catattgccg agatgggatg catcatattc
     9781 catgacgggg cgttgcctga tgatactgac caccagtggt ttgattaaca tattggtcgc
     9841 ggcccgttgt tgtataccgg cggcgaaaat gatctccaga gcattctgat cgataacacg
     9901 ggccagattc tcgttcagat tttcaatctc atgtccggtc agcaggaaac tgccggcaag
     9961 ccatgtcacc tgctcctgaa tcattgccag aattttgacg cgatcttctt caaccagcgc
    10021 tccggcaatt tttcctgata cagcggaggc aatcattccc cccagaacac cgccaaccac
    10081 acgccctgtc agtgcaccaa atggtcccag tggactgaac agtattccgc ccgccactga
    10141 cccgacagca ccacctgcca caccggaaga tgccacggca agattcctga tgaactgcgt
    10201 accggagatc cgtccccgca atattttgat catatcaggg ccggttgtga cagctaccag
    10261 tgccagagat gtcaccagcg tacccttcat cactttgttc agggcactga tatttcctgc
    10321 accggtcccc ttctgaagag ccagtcgaac agtcgggctg gcagtcgaaa aatcaatgtg
    10381 cttcagcata ccctgaacaa cactgagccg gtggagttgc tgggttgtga ggtagacagc
    10441 cagtgtgcgg gtgaaggttt ttcctgcctg gacagcagct gtctgcaggg cagcatcgcg
    10501 atcgccggtg ctgagccaga agaccgatgc cgtcagggca aaactgatcc ctccggccgc
    10561 cagactgaca accgaaccct cggcaatatc ataagtgacc gattcgatgg tcccgaaccg
    10621 ggtgatatta cgggcctgag tataagtcag atgtccccga cgaatcagcc gggatgcttc
    10681 tgcgggatcg gttacaccgg gtactttacc ttcgcggatc ttattcctca tggtttcaac
    10741 agcgccggca tactgatcac gggggacttc cagttgcatg gggccattat ttcccatgta
    10801 acgatactgt ccgttttgtc cgtcgaatgc cgcaccgacg ctacgggctg cagttgaaca
    10861 atatttcgtc tggatttctg ttccgctgac cagtctgtct gctccatttc tggcattatt
    10921 atcaccgagg atccgtgcgt ttttcagacg gactctgtcg acaatatggt ttccccgttc
    10981 agcggcaaac ccatgtccct gccctccgga agcaaacatt ttctcatttg tactccaggt
    11041 ttccggcagt ttcatgagaa tcagaccatt cgccgttgca tcggaaataa agctgttgta
    11101 agggcgttca taaatttcct gacacagtgt ttctgtttcg cgacaaagag aggagcagta
    11161 ccattccgga tccggcttat gattttccgg ggcatgatgt acttctgtca gcccgcagga
    11221 ggcacagaca cgaagctcat ccacgtgatg gaatgataca aaacggcgac cttctgaaaa
    11281 gggataccag tcaccgccgt ccaggcaacg atcgagaaaa tcgtcattcg cttccggtga
    11341 taaattcagg cggttccgga agagcgtttt cagttcatca ggtgtgaatg acaggttctc
    11401 tttcaactgt tcgttaaacc ctgccatgtt tctttcatac caggcattgt tcagtaacca
    11461 gtttccgtct gccagttcaa attccctcag ctctccccgg gaaatccgtt gagaaatgag
    11521 cgtcagccag atgcgctccg tcagtgagtc aggaaggctg taatcgtttt gcaaactggt
    11581 cagccacgat ttactgttga gcaccggttg ttcttccagt gtctggctga tggcacaggt
    11641 cagttttttc agcgcctgga tttggctggc cgcccatagc agcgtcacta atgcagccag
    11701 ccagtacaac aacccgcccc accacatcag tgacggtgaa atatcgtact gtaacgcgat
    11761 caatggtgcg catgcagaac acgccataag gaaaaaggca gtataaaaac agcagtaata
    11821 cccgttaatt tccttgctga tatcttctgg tgtgatcccg ttctgctgaa tgtttttgat
    11881 ctgcagggga acagccagca gaccaccggc gagagcggtg gtcagaataa atttcacaga
    11941 gtcaaaggtg tgagcctcca gcgcgatatg tttaccatca agtccccaca tgaagctgat
    12001 gatcattccg gcaatcgtca gaaatacgcc tgccagccag cgaatatgga acaaccgggt
    12061 tatgcgttcg gtgatatagc cagccagtaa ggccgggccg aacagacaca gcaatatgag
    12121 ccctgccagc gcagaagccg tgctgattaa tgctctgaac aaaaggcgaa gaatggcaaa
    12181 tcctgcgatc agcaggatca gcgcgccgac tatctgtaac atagtcactc cgtgatgaat
    12241 atcatgttat tgttaatgcc agcgaatgtg gcgttgaata tccgttgcgc cggtccgggt
    12301 cccgatcatg atgaccggga ccacagagag gcgatggttc tgtcagaagg tcacattcag
    12361 cgtggcctga ccgttatagc cttcagcgct gctgccgctg acgctgtgga cataacctgc
    12421 ctgaacgccc agggtgatat tttcccggac acgggcttcc agtccggcct gcaggtccag
    12481 tgacgtacca ttctgtgacg gtgagaacgt catgttactg ccggctgcgg ctgtacccat
    12541 gctcatgtcc ccccgggagc tgaaggtgcg gataacagaa ggctgtaccc accagttcac
    12601 cggcagttca cgcacactgt gtttggcact gccgcgcagg gtgtcacggg atgaggtgcc
    12661 tttaccaaag ttcatatcgt ggtggctgcc cagacggaag ccggcacgca catgttgtgc
    12721 actgccatgc ccgaacttca cataactggc gttatcctgg ccgtcatcca gggagagtcc
    12781 ctgccaggtg tactgcagtt gcggctccag catcagattg tcagtgatac tgaagggcag
    12841 accggtttcc agcgaaccca gccagcccca gccccggacg cggaagtcgt tattgtccga
    12901 tgaggctttc atgctgtggc gggttccctg ggccacaatg tcagcccaca ggccggaggc
    12961 gttgtgtatc agattcaggt atccgcccag gctgccggca tcatcccgga ccgtgccggc
    13021 acgggagccg tcatcatcct taacatcaac ggaagaatgg ccagcagcac catatacccc
    13081 cgcggtcaca gacataccgg caacctctgt tctgagcagg tcaccctcca gacggacgaa
    13141 gccatagctg ccgctgcttt ccggcgtggc tccacgggca ataccgccgt tgttatcgtg
    13201 accgagatga ccgccctgaa tgctgagacg gacgctgtta ttttcaccgc ttacaccggt
    13261 ctgatggctg cgggagcctg ccagaatccg gtcatagtcc attgcctgtg tcagcatgga
    13321 ggcatacagg gggacttctg cacgataagc attttcactg cgcagatacc agctctcatc
    13381 actgtcccgg ttgagggagt agttaaaggc accggcctgc agcttattcc cctggataaa
    13441 ggccccttcc tccgtggtgg caccgttaat ggcttccacc acctgaatac ccttaccgct
    13501 ggtcgccagc cccgacgcac tgttgccggc gttcaccagg ttcaggatgg tttttccggt
    13561 tgccctgcca ccgtcaatga ccagtctgtc agcattgttc tgtgccatat ccgggcgtac
    13621 acgcaggctg atggtgccat tctgtccgtt caggtttttc actttcaggg ttgccggtac
    13681 gaacttccct gtgcgggtgg aggtgaaatg aatctgtccg gcatggctga ggtcatccac
    13741 caccgactgc accgtggcgt tatcgggaat attccaggtg gcaccagagg cgagagtgac
    13801 attcgtgggg tcaatggcac cgttcagcac agtgctgccg gtcagcttca gggctgtacc
    13861 gcgctgagca atgacatccg tggtgacggt actgtcgttc agcgtcagcg tgccttcatt
    13921 caggttgacg gctttctggg tgagtgtggt gttgctgaca gtgagtgtgc cacttcctga
    13981 tttttccacg ctgccgttac cggtgagaga gcctccctgc aggagtgcat cgccttcacg
    14041 gatggtcagg gtatcgttgt tcaccgtctt cccggaaagg gtaagtatgg cgccgttatt
    14101 cagcgtggtg gtgcccgcca gtgtgccgcc cctggcggtg aacagtccgc catttaccgt
    14161 ggtatccgtg gccgtatcac cggcgttcag cgtgaatgaa ctgccttttt ccagcatcag
    14221 ggcatccgcc tgaccacctc cgatactgaa tgcctttccg tcgctccggg taccactgac
    14281 agcggcaccg gaatcggcca gcagtacacc gccatttccc atggatacgg tggtggcggt
    14341 gccaccgttg cggacatcca gcgttccgcc atcatccacg cgggtattag tggctgtgtg
    14401 tccggtcagc acatccaggc gtccgccatt ttccagtacg acattatcag ctttaccctc
    14461 cacaacagag aatgctccca ggcggtttat gccggtaacg gttgcagccg tactggtaac
    14521 cagtgcgccg ccctgcttca gggtgacatt cgtggctgta ccaccggcgt ccacctgcag
    14581 tctgcccttc tgattaacgg tggtattccc ggccacaccc ccgtttttga caatctgcca
    14641 gccgtcactg tttacaacag tgccagacgc cgtaccgccg ttgtgcacat actggtaccc
    14701 cccattcagc gtggtatcca gtgcgtgacc atgtacagtc tggtcgccgc cggcataaac
    14761 cacagtggta tttgccgttc cttcagcagc cacaatctga cgaccatttt tattgatggt
    14821 ggtgcgtacg gcatctccgt agacggtctg cccggtatca ccattttccg catccggtcc
    14881 cccttccgcg ccggtattca caaccgtgtc ggttgccact gcgccggatt tgatggcctg
    14941 ccagcccttc tcattaatga cggtacccgt tgcaatcccg ccttcatgta cccactgctc
    15001 accgccgttc agagtggtgt tcactgcctg cccctgaagg ctctgtccgc ctccggcact
    15061 gataaccgtg tctgaaacgc ttcctccggc attcactctc tgaagaccac caccggtgac
    15121 agtagtgttg ttggcgatac cgccattttg tatccattgt ccgccggtat tggcctcgtt
    15181 atccggccca tactccagac cggtactgat ggtcattccg ttggccgtac cgaggacaat
    15241 ctggttgtca tgatttgtca gtgttccgtc gttcacggtt tctcccgcct gtacaacctt
    15301 gtcagcagcc agtgccggga ctgatgtgac agcagcaaga gacagcgcaa ccgccacacc
    15361 agcgcgtttt ccccgtgagc gggccagttc ggaggccacc accagggtgc ccgtaatgtg
    15421 attccatacc agcctgtagc tggtgttcag atgtcgtttc atcagctttt ccttacagag
    15481 ggtgaataaa aaagccggta cccacaaatg tggataccgg caggtacaaa acagcatgct
    15541 cagataaagt ggtttttctc ggacataaat gcttcccctc tctcatccgc cagagaagta
    15601 tcagtccgtt gtgtgcaatt ccgttctgca tgatgaagag attcagctgc cttttcgcag
    15661 accattaacc actataaacg atcgatagca acgatcgata cctgttttat cgatcgtttt
    15721 attctattaa cgacaaggcg gtcacgcaag agaaacagga ggagtatgag gggtttgtgt
    15781 catgttttca gttggttaac gtaatgttat caggcatttc cctggcagac tgttcagaag
    15841 aaaatccagt tccatacagc gcgggcaaca gagaccaccg tgtcacggac ggcacgcaga
    15901 accgtacggg caacagaggc aacacagacg ctctccgccg tgtcaaatat ccggtccacc
    15961 gcaccggtaa actgttcacg ggcctgagcg cggacagatt ccgtgcgcag ctcgtcctgc
    16021 agacgggtca tcaggggact ggcggcatgg tcgggaagtg ctgtcattag cgcactgacc
    16081 agcgtatcca gttcccagcc ggtgcgggcc gatacggcaa caaccggatg tacgggccgg
    16141 aacagacgga ataccgcctc cgttttctcc cgaatgttct gtgcctgtgc gggagaaggc
    16201 tgaatgccgg ccatatccca ttcatggcag ggctccgttt tgtcggcctg cgtcaccaca
    16261 aacagcacct gctgatgtcc ccggtgcagg atgtgtcgcc agaaatactc atccacagac
    16321 agggcgcggt catcggcttt aatcagccac agcaccaggt ccagttcggg cagaatgtca
    16381 cggtacaggg cttcatactc tgcatccctg tcccggctct cgcccacccc gggcaggtca
    16441 gtgataacca tgctgtgacc gtggccactc agacggaagc gccgcacttc ccgggtgccg
    16501 gcgtgaacat cactgaccgg ggtgacctcc ccctgaaaca gtgcattaca gagtgaggat
    16561 ttaccggccc cgcttttacc cataatgcca atcacgggtt cgtgactggt gagtttgcgc
    16621 agatgttcca ggatgtgacg ggaaagtgag taaggcaggg aggagagcgg tttttcaatt
    16681 gcctcaatgg catcagacgg attcatacat gtattcccgg tgaaaaaaga caaaaccccg
    16741 cgcaccggaa aggtggcggg ggattcacgg agataatatc tgtctgaaat tgttttgaat
    16801 cgatgggatt gttgcgtatt aacatgaagg agatcaggac ttatccgtta ctgggtgatc
    16861 tccttccatg aaaatacgcc gttattgtta aacagcaggc tgatttctcc gtaaagggga
    16921 aaatatttat cggctgtatg attatcaggt gtgtctgtgt ttactgctgt gagtattttt
    16981 tagtgtattg tgggggagat gtcagtggga gatattaaat ttacgtttat aattatctta
    17041 cttcttctgt gtgtttttta taagagtgca cacgtaatga tatctgttga taccatgtgg
    17101 ttaagtgttt cccttcctgg tcatgcgtaa tgattaaatg cttttgtgac accattcttc
    17161 cggtctgaat atttatcccg ttctttttta tttgtgcagt gatactgtcg gaagagaatt
    17221 cagtaatgtg aggtgtcctg gtcaggataa tatctgctga gatattatta gttgagtgtg
    17281 ttttactgag caaaagctgg cttttaattc ctgtactgaa acactgtcgc tcgtccgggg
    17341 ttaactgaaa ctggatgatg atgtaaaatg aaggctgttt ttgtgtttgt cgaagaagtg
    17401 ttattttaat ccattctcat cattggctgg cgattataca gaagtatttg aagaaaaaat
    17461 ggtgcatttt atctgtgtca gcagggaaga
//
LOCUS       DAPGOF010000100        17448 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.101, whole genome shotgun sequence.
ACCESSION   DAPGOF010000100 DAPGOF010000000
VERSION     DAPGOF010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.101"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            135..1346
                     /gene="mhpT"
                     /locus_tag="RHV13_002942"
     CDS             135..1346
                     /gene="mhpT"
                     /locus_tag="RHV13_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HDV1242457.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            1448..1987
                     /locus_tag="RHV13_002943"
     CDS             1448..1987
                     /locus_tag="RHV13_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HDV1242458.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(2213..3046)
                     /gene="fghA"
                     /locus_tag="RHV13_002944"
     CDS             complement(2213..3046)
                     /gene="fghA"
                     /locus_tag="RHV13_002944"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HDV1242459.1"
                     /translation="MELIEKHASFGGWQNVYRHYSASLKCEMNVGVYLPPKAAGEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIIVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMNHFPATAKKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(3139..4248)
                     /gene="frmA"
                     /locus_tag="RHV13_002945"
     CDS             complement(3139..4248)
                     /gene="frmA"
                     /locus_tag="RHV13_002945"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HDV1242460.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVADVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            complement(4283..4558)
                     /gene="frmR"
                     /locus_tag="RHV13_002946"
     CDS             complement(4283..4558)
                     /gene="frmR"
                     /locus_tag="RHV13_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HDV1242461.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            complement(4718..5764)
                     /gene="fbpC"
                     /locus_tag="RHV13_002947"
     CDS             complement(4718..5764)
                     /gene="fbpC"
                     /locus_tag="RHV13_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric ABC transporter ATP-binding protein"
                     /protein_id="HDV1242462.1"
                     /translation="MSQKNFVELRNVTKRFGSNTVIDNINLTIPQGQMVTLLGPSGCG
                     KTTILRLVAGLEKPSEGQIFIDGEDVTHRSIQQRDICMVFQSYALFPHMSLGENVGYG
                     LKMLGVSRSEVKQRVKEALAMVDLEGFEDRYVDQISGGQQQRVALARALILKPKVLLF
                     DEPLSNLDANLRRSMRDKIRELQKQFNITSLYVTHDQSEAFAVSDTVLVMNKGHIMQI
                     GSPQDLYRQPASRFMASFMGDANLFPANFSEEYVDIYGYRLPRAAHFPAQGSGTVGVR
                     PEAITLSNHGEESQRCVIRHVAYMGPQYEVTVEWHGQEILLQVNATRLQPDIGEHYYL
                     EIHPYGMFVLADAA"
     gene            complement(5776..7854)
                     /locus_tag="RHV13_002948"
     CDS             complement(5776..7854)
                     /locus_tag="RHV13_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDV1242463.1"
                     /translation="MSQTLALHPVKKRDAIFLWVLLGWLAFILLPSWSLDYGLLESTS
                     DEILEAYGWSHFNISWLWYLLPSLLLVRPFREATREQRSRHYFDASWALLCMAFIVIS
                     ATVAGRGLGYAAIVLFVELGAIMTLALTRLEWLGGDRFVIGSLIAIVALIGIFIVWPS
                     IAIFIPMFTNDAGEFAPLAFMTVLSQAHIVQVIVNSIVLSIAVGIGCTFFGLVLAIYT
                     TRIAKRSAIIGRIFSILPIVTPPFVVGLGVTLMMGRSGYVTEFMVEWFGLTNTNWLYG
                     FTGIWLAQVLAFTPMAFMILDGAIKTIHPSLEEASYTLRASRWQTFNGVFVPLLKPAL
                     ANAFLIVVVQSLADFSNPLVLGGNFDVLATQIYFYITGSQLDYQAASTLGAFLLLFSL
                     LVFCIQYMWIGKRSYVTVSGKSYRGDVQPLPVTLVWSVVAILAIWIAFNALLYGSIFY
                     GSFTVNWGVDYTLTLDNFIKLFGQGMSDGAWPSLLDTLLYAGIAAPITAIFGLLIAWV
                     VVRQQFKGKKTIEFTTMLCFAVPGTVAGVSYILAFNSAPVYLTGTAAIVIISMVMRNV
                     PVGIRAGIAGLGQIDKSLDEASLSLRAGSLRTITHILLPLLRPAILSALIYSFVRAIT
                     TVSAIVFLVTPDTRVATAYILNRVEDGEYGVAIAYGSILIVVMLAIIFLFDWLIGEAR
                     ISRSKAKNQA"
     gene            complement(7923..8954)
                     /locus_tag="RHV13_002949"
     CDS             complement(7923..8954)
                     /locus_tag="RHV13_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDV1242464.1"
                     /translation="MKMTFTSTLIASAVALATLTGAAQAKGRLVVYCSATNEMCEAET
                     KAFGEKYDVKTSFIRNGSGSTLAKVDAEKKNPQADVWYGGTLDPQSQAGEMGLLQAYK
                     SPNLEQIMEKFRDPAKVKGNLSSAVYVGILGFGVNTDRLKEKNLPVPKCWKDLTKPEY
                     KGEIQIADPQSSGTAYTALATFAQLWGEDQAFDYLKQLNANVSQYTKSGIAPARNAAR
                     GETAIGIGFLHDYSLEKEQGAPLELISPCEGTGYEIGGVSILKGARNLDNAKLFVDWV
                     LSKEAQELAWKKGKSYQILTNTTAETSPNSLKLDDLKLINYDMDKYGSTDVRKALINK
                     WVSEVKMGK"
     gene            complement(8951..10267)
                     /gene="uhpC"
                     /locus_tag="RHV13_002950"
     CDS             complement(8951..10267)
                     /gene="uhpC"
                     /locus_tag="RHV13_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HDV1242465.1"
                     /translation="METTMHARSAREIDQCYRTLRPRLLMCMIIGYAAFYLTRKSVNY
                     VLPALQTDLGLDKGDIGLLGSLFYLSYGLSKFTAGLWHDSHGQRGFMGVGLFATGLLN
                     VVFAFGESLTLLLVVWTLNGFFQGWGWPPCARLLTHWYSRNERGFWWGCWNMSINIGG
                     AIIPLISAFAAHWWGWQAAMLTPGIISMALGIWLTLQLKGTPQEEGLPTVGHWRHDPL
                     ELRQEQQSPPMGLWQMLRTTMLQNPLIWLLGVSYVLVYVIRIALNDWGNIWLTESHGV
                     NLLSANATVMLFEVGGLLGAVFAGWGSDLLFSGQRAPMILLFTLGLMVSVAALWLAPV
                     HHYALLAVCFFTVGFFVFGPQMLIGLAAVECGHKAAAGSITGFLGLFAYLGAALAGWP
                     LSLVIERYGWPGMFSLLSVAAVLMGLLLMPLLMAGITTTHARRIKQ"
     gene            complement(10340..11881)
                     /locus_tag="RHV13_002951"
     CDS             complement(10340..11881)
                     /locus_tag="RHV13_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HDV1242466.1"
                     /translation="MSRNLRHWVISLFIVLAWGTGWLMLWTLSFYLTNNGQQAVLFLP
                     QGVYLALVILLSRRYWPALVLPPLLMMFWLHSEQLLNGYLMLATPVISLFPVLLAQNF
                     WHRFPLYWQRLTLLLATVTAASLLNTALLSPFMSGPIMLPGLTSFTGGVLLTPFVYLI
                     FEFLRQQHRYQLLGLDTHNPPLRTSLIIWCSLFFIIGIGTQMVLSPEIERLLLIVVFL
                     PNVVMAWKFGWQGGVLSGLLGSMMITIARQIGVGFSNLVELEIFLATQALLGIGLGIA
                     ISRQQHLALNLHHYRQRLEAELAARRALAEKLIHTEEDTRKNLARELHDEIGQNITAI
                     QIQSQLVKRARDPAQIQSAASQINELARRIHLSTRQLLRQLRPPALDELTFREALLHL
                     INEFAFSERGIHCQFAYQLNSTPENETVRFTLYRLLQELLNNICKHAEASEVTIILRQ
                     QGEVLHLEVSDNGVGIASGKMAGFGIQGMRERVSALGGDLTLEKQHGTRVIVNLPTFL
                     QQIAG"
     gene            complement(11881..12510)
                     /locus_tag="RHV13_002952"
     CDS             complement(11881..12510)
                     /locus_tag="RHV13_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDV1242467.1"
                     /translation="MIRVVLVDDHVVVRSGFAQLLSLEDDLEVIGQYSSAAQAWSALI
                     RDDVNVAVIDIAMPDENGLSLLKRLRAQKPQFRAIILSIYDAPTFVQSALDAGASGYL
                     TKRCGPEELVQAVRSVGLGGHYLCADAIRALRGGGQPAQALEILTPREREVFDLLVKG
                     DSVKEIAFKLELSHKTVHVHRANVLGKLNCHSTIELVHFALDHNLLAGH"
     gene            12813..13775
                     /gene="tauA"
                     /locus_tag="RHV13_002953"
     CDS             12813..13775
                     /gene="tauA"
                     /locus_tag="RHV13_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV1242468.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            13788..14555
                     /gene="tauB"
                     /locus_tag="RHV13_002954"
     CDS             13788..14555
                     /gene="tauB"
                     /locus_tag="RHV13_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HDV1242469.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            14552..15379
                     /gene="tauC"
                     /locus_tag="RHV13_002955"
     CDS             14552..15379
                     /gene="tauC"
                     /locus_tag="RHV13_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HDV1242470.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            15376..16227
                     /gene="tauD"
                     /locus_tag="RHV13_002956"
     CDS             15376..16227
                     /gene="tauD"
                     /locus_tag="RHV13_002956"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HDV1242471.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLSFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(16334..17308)
                     /gene="hemB"
                     /locus_tag="RHV13_002957"
     CDS             complement(16334..17308)
                     /gene="hemB"
                     /locus_tag="RHV13_002957"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HDV1242472.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         4132 a         4643 c         4765 g         3908 t
ORIGIN      
        1 cacgactgcc ggatgcggcg tgaacgcctt atccggtcta catttcgccc gtaggcagtc
       61 attaaatggt tctgattacg cggctggcaa tgtatcagtc gcgattcacc tgcactcgca
      121 atgaggttca tcttatgtcg actcgtaccc cttcatcatc ttcatcccgc ctgatgctga
      181 ccatcgggct ttgttttttg gtcgctctga tggaagggct ggatcttcag gcggctggca
      241 ttgcggcggg tggcatcgcc caggctttcg cactcgataa aatgcaaatg ggctggatat
      301 ttagcgccgg aatactcggt ttgctacccg gcgcgttggt tggcgggatg ctggcagacc
      361 gttatggtcg taagcgtatt ttgattggct cagttgcgct gtttggtttg ttctcactgg
      421 caacggcgat tgcctgggat ttcccctcac tggtctttgc gcggctgatg accggtgtcg
      481 ggctgggggc ggcgttgccg aatcttatcg ccctgacatc tgaagccgcg ggtccacgtt
      541 ttcgtgggac ggcagtgagc ctgatgtatt gcggtgttcc cattggcgcg gcgctggcgg
      601 cgacactggg tttcgcaggg gcaaacttag catggcaaac ggtgttttgg gtaggtggtg
      661 tggtgccgtt gattctggtg ccgctattaa tgcgctggct gccggagtcg gcggttttcg
      721 ctggcgaaaa acaggctgcg ccaccactgc gtgcgttatt tgcgccagaa acggcaaccg
      781 cgacgctgct gctgtggttg tgttatttct tcactctgct ggtggtctac atgttgatca
      841 actggctacc gctgcttttg gtggagcaag gattccagcc atcgcaggcg gcaggggtga
      901 tgtttgcact gcaaatgggt gcggcgagcg ggacgttaat gttgggcgca ttgatggata
      961 agctgcgtcc agtaaccatg tcgctactga tttatagcgg catgttagct tcgctgctgg
     1021 cgcttggaac ggtgtcgtca tttaacggta tgttgctggc cggatttgtc gcggggttgt
     1081 ttgcgacagg tgggcaaagc gttttgtatg ccctggcacc gttgttttac agttcgcaga
     1141 tccgcgcaac aggtgtggga acagccgtgg cggtagggcg tctgggggct atgagcggtc
     1201 cgttactggc cgggaaaatg ctggcattag gcactggcac ggtcggcgta atggccgctt
     1261 ctgcaccggg tattcttgtt gctgggttgg cggtgtttat tttgatgagt cggagatcaa
     1321 gaatgcagcc gtgtgccgat gcctgacgcg ccttattaag caggggcgaa agggacttgc
     1381 ccccaataca cccacggcgt ttacaggtat actcgctaaa aattattcag cgggtttgga
     1441 aacaaagatg gcaaaactta ccttacaaga gcagttgctc aaagcaggat tagtcaccag
     1501 caaaaaagcg gcgaaggtcg agagaacggc gaaaaaatcg cgtgttcagg cgcgtgaagc
     1561 tcgggcggcg gtagaagaaa ataaaaaggc acagcttgag cgtgataaac agcttagcga
     1621 acagcaaaaa caagcggcgt tggcgaaaga atataaagcc caggtgaagc agctcattga
     1681 aatgaaccga atcaccattg ccaatggcga tattggtttt aacttcactg acggcaatct
     1741 gattaagaag atttttgtcg ataagctcac acaggcgcag ttgattaatg gtcgtctggc
     1801 gattgcccgc ttgttggtcg ataacaatag cgaaggtgaa tacgccatta ttcccgccag
     1861 cgttgccgat aaaattgctc agcgtgatgc cagcagtatt gtgttgcaca gcgcgctcag
     1921 tgccgaagag caggatgaag acgatccgta tgccgacttc aaagtgcctg atgatttgat
     1981 gtggtaaaca tagataagtc gtgcgcagat gcctgatgcg acgctatgcg cgtcttatca
     2041 ggcctacggt ttatgggcga agtgtaggcc ggataaggcg ttcacgccgc atccggcagt
     2101 cgtgcgccat gcctgatgcg acgctttgcg cgtcttatca ggcctacggt ttacgggcga
     2161 agtgtaggcc ggataaggcg ttcacgccgc atccggcagt cgtgcactat tatcaacgca
     2221 tattcagttt attggcgtgg taggcaatat gctcgccaat aaaactggag acaaaataat
     2281 agctgtggtc gtaaccctcg tgataacgga ttaacgtctt gatattcatc tcctggcaga
     2341 tcttttcaag atttggagtc cgcagctgtt ccgcgtaaaa atcatcactc aacccctgat
     2401 caaccatgat ttccgcaacg cgttgacctt gtgaaataag actcaccgga tcgtaatcca
     2461 accaggcatc tttattttca ccaagatatg cggcaaaggc ttgctgtccc cacggtactt
     2521 gcgatgggga gacaatgggc gaaaacgccg agacgctgac atattcatct gggttacgta
     2581 acgccagcac cagcgcgccc agtccgccca tagaatgacc agagatagac tttttggccg
     2641 ttgccggaaa atgattcatc actaaatccg gcagctcgtt gcggatatag tcatacattt
     2701 tgtaatgttc attccacggc gcttgcgtcg cgttcaggta aaacccggca ccttgcccga
     2761 gatcgtaacg gtcggcatct gcgacatgac tgcctcgcgg actggtgtcc ggcgcaacaa
     2821 taataatgtt gtgctcagcc gcgtaacgct gcatccccga tttagtaatg aaattctgct
     2881 cattgcaggt caggcccgaa agccagtaca gcaccggtaa tttttcacct gcagcttttg
     2941 gtgggagata gacgccgaca ttcatttcac atttcagtga tgcggaataa tgccgataca
     3001 cattttgcca gccgccaaag ctggcatgtt tttcaatgag ttccatcagt cattatctca
     3061 cggttaatcc cccggacggc tgcccgggga gagtattaag aaccccgcct gaactggacg
     3121 aggtaaacca gcgggaaatc agtaacgaat tacggttcga atggatttgc cttcatgcat
     3181 caggtcgaag gcgtcattaa tttcatccag gctcatggta tgcgtgacaa acggttccag
     3241 atcgatatca cctttcatcg catcttcaac catacccggt aactgggaac gacctttcac
     3301 gccgccaaac gcggaacctt tccatacgcg accagtgacc aactggaatg gacgggtgga
     3361 gatttcctga ccggaacccg cgaccccgat gattaccgac tgaccccagc cgcggtgcgc
     3421 actttccagc gccgcacgca tcacgttgac attaccgatg cattcaaagg tatggtcgat
     3481 accccatttg ttgatatcca gcaggacatc tttaatcggt ttgtcgtagt cattcgggtt
     3541 aatgcagtcg gtggcaccga agcgacgcgc cagatcgaat ttcttcgggt tggtatcgat
     3601 agcgataatc cgtcccgctt tcgcctgacg cgcgccctga accactgcca gaccaatcgc
     3661 gccaagacca aacacagcaa cagaatcccc tggctggact ttagctgtgt tgtgtaccgc
     3721 gccaataccg gtggtcacgc cacagcccag caggcagacg tgttcatggt ttgcttctgg
     3781 attaattttt gccagagaca cgtccgcgac tacggtgtat tcactgaatg tagaacaccc
     3841 catgtagtga taaagcggct gcccgttgta agaaaaacgg gtggtgccgt ctggcatcag
     3901 gcctttaccc tgggtttcgc gaaccgcaac acagaggtta gttttgccag aacgacagaa
     3961 ctcacactcg ccgcactccg cggtgtaaag cgggatcaca tggtcgcctg gtttgacgct
     4021 ggttacgcct tcaccgactt caaccacaac gccagcccct tcgtgaccga gaaccaccgg
     4081 gaatacacct tccgggtcat cgccggagag ggtaaatgcg tcggtgtggc aaacgccggt
     4141 atgggtgact ttaatcagca cttcaccttt tttcggtggt gcaacgtcaa tttcaacgat
     4201 ttccagtggt ttaccgggag caaatgcaac ggcagcacgt gatttcatct ctcgctcttc
     4261 ctcaatatgg taatagattc agctatttaa gataggcacg aaccagttca atagtgtcgt
     4321 caacggattg gctgacttcg cggctgtagc agtcatttcg gtcaaacgtt tcccggatat
     4381 ggctttcaag cacttctgcc atcagcccat tagccgcgcc ccgaacggca gcgatctgtt
     4441 ggagtatggc acggcattcg gcatcaccct ccagcgaccg ttccagagca tcaatctgcc
     4501 cccgaatacg acgaactcga gtaaggacct ttttcttctc ttccggagta ctgggcattt
     4561 cgcacctcat catctgcacg caatatacta taggggggta tgctatatgt caatgcatac
     4621 ccccctatag tatataacac tggagaataa ttgttgtccg aagcctaacc aggtgaacgg
     4681 tccctcaaga cagatgggga ccgtttatgt gctattttta tgccgcatcc gctaaaacaa
     4741 acatcccgta aggatggatt tcgaggtaat agtgctcacc aatatcgggc tgtaaacggg
     4801 tggcgtttac ttgtaataaa atctcctgtc catgccactc tacggtcact tcgtactgcg
     4861 gccccatgta ggcgacatgg cgaataacac aacgctgact ctcttcgccg tgattgctta
     4921 aggtgatggc ttccgggcga acgccgacgg tgccgctacc ttgcgccggg aaatgcgccg
     4981 cgcgcggcag gcgataaccg tagatatcga catactcttc gctaaagttc gccgggaaca
     5041 ggttggcgtc gcccataaaa cttgccataa atcgggaggc tggctgacga tagagatcct
     5101 gcggtgagcc aatctgcatg atgtgacctt tattcatcac cagcacagtg tcggacaccg
     5161 cgaaagcttc actttgatcg tgagtgacgt agagcgacgt gatattaaac tgcttttgca
     5221 gctcgcggat cttatcgcgc atactgcggc gcaggttggc atcgaggtta cttaacggct
     5281 catcaaacag cagcaccttt ggcttgagga tcaacgcgcg ggccagtgcc acacgctgtt
     5341 gctgaccacc ggaaatctga tcgacatagc ggtcctcgaa gccttccaga tccaccattg
     5401 ccagcgcctc cttcacccgc tgtttcactt cgctacgcga gacgccaagc atcttcagcc
     5461 cgtagccaac gttttcaccc agcgacatat gcgggaacag agcgtatgac tgaaacacca
     5521 tgcagatatc gcgctgttga atggaacgat gcgtgacatc ttcgccatca ataaaaattt
     5581 gcccttcgct cggtttttcc agcccggcaa ccagacgcaa aatggtggtt ttgccacagc
     5641 cggaaggacc gagcagcgtc accatttgcc cttgtggaat ggtgagattg atattgtcga
     5701 taaccgtatt actgccgaat cgtttagtga cgttgcgcag ttcaacaaaa tttttctgac
     5761 tcatagtgcg ctccattacg cctggttttt ggctttagaa cgggagatac gagcttctcc
     5821 gatcagccag tcaaagagga aaataatcgc cagcatcacc acgatcagaa tcgaaccata
     5881 ggcgatggct acgccatatt cgccgtcttc aacgcggtta agaatgtagg cggttgccac
     5941 gcgggtgtcc ggcgtgacga gaaagacgat ggcgctgacc gtcgtgatgg cccgcacaaa
     6001 gctgtagatc agggccgaga gaatggccgg acgcagcagc ggcagtaaaa tgtgggttat
     6061 ggttcgcaag gaacccgcgc gcaggctgag tgaggcttca tccagcgatt tgtcgatctg
     6121 ccccagaccc gcaatcccgg cgcgaatacc caccggaacg ttacgcatca ccatcgagat
     6181 aatcacaatt gccgccgttc cggtgagata aaccggagcg ctgttaaagg cgagaatata
     6241 cgagacgccc gccaccgtac ccggtacggc aaagcacagc atggtggtga actcgatggt
     6301 ctttttgcct ttgaattgtt gacgcaccac cacccaggcg atgagcaggc caaagatagc
     6361 ggtaatggga gcggcaatcc cggcgtagag cagggtatcc agcagtgaag gccacgcgcc
     6421 gtcgctcatt ccctgaccaa atagcttgat aaagttatcc agcgtcaggg tgtagtccac
     6481 gccccagttc accgtgaagc tgccgtagaa aatgctgccg tagagcaggg cgttaaaggc
     6541 aatccatatc gcaagtatgg cgaccacgct ccacaccagg gtgaccggta gcggctggac
     6601 gtcgccacgg taggatttgc cggaaaccgt gacgtaagag cgtttaccaa tccacatata
     6661 ctggatgcag aacaccagca gcgagaacag cagcaggaaa gcgccaaggg tgctggcggc
     6721 ctgataatcc agctgcgagc cggtaatata gaagtaaatt tgcgtcgcca gtacgtcgaa
     6781 gttaccgcca agtaccagcg ggttactgaa atcagccagt gactgtacca cgacgattaa
     6841 aaaggcattg gcgagtgcgg gcttcaacaa cggcacaaac acgccgttaa aggtttgcca
     6901 gcggctggcg cgcagggtgt aggaggcctc ttccagcgag ggatgaatgg ttttaatggc
     6961 cccgtcgagg atcataaatg ccattggcgt gaatgccaga acctgtgcca gccagatccc
     7021 ggtaaaaccg tacagccagt tggtgttggt cagcccaaac cactccacca taaattccgt
     7081 aacatagccg gagcgcccca tcatgagcgt cacgcctaag ccgaccacaa acggtggcgt
     7141 aacgatgggc aaaatggaaa agatgcgacc aataatggcg ctgcgtttag cgatgcgcgt
     7201 ggtataaatc gccagcacca ggccgaagaa agtacagcct atccccaccg ctatcgacaa
     7261 gacgatagag ttgacgatca cctgaacaat atgtgcctgc gagagcacgg tcataaacgc
     7321 cagcggggcg aactctcccg cgtcgttggt gaacatcggg ataaaaatgg cgatgctcgg
     7381 ccagacgata aaaatgccaa tcagcgccac aatcgcaatc agtgagccga tcacgaagcg
     7441 gtcgccgccc agccattcga ggcgggttaa ggccagcgtc ataatcgcgc ctagttcaac
     7501 aaacagcacg atggcggcgt agcctaaacc gcgtccagcc acggtggcgc tgatgacgat
     7561 aaacgccata cagagcaatg cccagctggc atcaaaataa tgacgactac gctgttcgcg
     7621 tgtggcctca cgaaacgggc gaaccagcaa cagactcggt aacaaatacc acagccagct
     7681 gatattaaaa tgcgaccagc cataagcctc gaggatctca tcactcgtgg actccagcag
     7741 gccataatcg aggctccagc tcggcaacag tataaatgcc agccagccca gcaaaaccca
     7801 aaggaaaatc gcatcccgct ttttcacggg atggagtgca agtgtctgtg acatagcagt
     7861 tccggtatgg ttggccgggg caggcgtttg tgctgcaccc ggtaaatacg cctaatgcgg
     7921 gtttatttac ccatcttcac ttcgctgacc catttattaa tcaatgcctt acgaacatcc
     7981 gtggaaccat atttatccat gtcgtagttg attaatttca ggtcgtcgag cttcagcgaa
     8041 tttggcgacg tttcggcggt ggtattggtc aggatctgat aagacttccc tttcttccac
     8101 gccagttcct gagcttcttt tgataatacc cagtccacaa acagtttggc gttgtcgagg
     8161 ttgcgcgcgc ctttcagaat gctgacgccg ccaatttcgt agcccgtacc ttcacacggg
     8221 gaaatcaact ccagcggcgc gccctgttct ttttccagcg agtagtcatg caggaagcca
     8281 ataccaatcg ccgtttcgcc acgggcggcg ttacgtgccg gggctatgcc tgatttggtg
     8341 tactgagaga cgttggcgtt cagttgtttt agataatcaa aggcctgatc ttccccccac
     8401 agctgcgcaa aagtagccag ggcggtgtag gcagtaccgg aactttgcgg atcggcaatt
     8461 tgaatttcgc ctttatattc cggtttggtg agatctttcc agcacttcgg caccggcagg
     8521 tttttctctt tcagacgatc ggtgtttacg ccaaaaccga ggatccccac ataaacggca
     8581 gaggacagat tccctttcac ttttgccgga tcgcggaatt tctccatgat ctgctcaagg
     8641 ttaggggatt tatacgcctg tagcaggccc atctcgccag cctgcgattg tgggtctaaa
     8701 gtgccgccgt accagacgtc agcctggggg tttttctttt cagcatcgac ttttgccagg
     8761 gtgcttccgg aaccgttgcg gataaacgag gtttttacat catatttctc gccaaacgct
     8821 ttggtttcgg cctcacacat ttcattggtg gcgctgcaat agaccaccag ccggcctttt
     8881 gcctgcgcgg cacccgttaa agtcgccagc gcaacggcag aagcaatcag agtagaggta
     8941 aaggtcattt tcattgtttt atccttcgag cgtgagtggt ggtgatgccc gccatcagta
     9001 acggcatcag cagtaaaccc ataagtactg cggcgaccga gagtaaactg aacattcccg
     9061 gccagccgta gcgttcgatg accagcgaca gcggccagcc tgccagcgct gcgccaaggt
     9121 aagcgaacag gccgagaaat ccggtgattg aacccgccgc agctttgtgc ccacactcca
     9181 ccgccgcaag tccaatcagc atttgtgggc caaagacgaa aaagcccacg gtaaaaaagc
     9241 acactgccag cagagcgtag tgatgaaccg gtgccagcca cagtgcggcg actgacacca
     9301 tcaaccccaa cgtgaacagc aaaatcattg gcgctcgttg cccgctaaac agcaaatccg
     9361 atccccatcc ggcaaatacc gcgccgagta agccgcccac ttcaaacagc attaccgtgg
     9421 cgttggcgct gagcaggttg acaccgtggc tttccgtcag ccagatattg ccccagtcgt
     9481 taagcgcaat ccggatcaca taaaccagta cataagagac gcccagtagc cagatcagcg
     9541 gattttgcag catggtggtg cgcaacattt gccacagccc catcggcgga ctttgttgtt
     9601 cctgacgtaa ctccagcgga tcgtgacgcc agtgaccaac tgtcggtaat ccctcttcct
     9661 ggggcgtgcc tttgagttgt agcgttaacc agatccctaa tgccatgctg attatccccg
     9721 gtgtcagcat cgcggcctgc cagccccacc agtgggcggc aaaggcgcta atcagcggaa
     9781 taatcgctcc gccaatgttg atcgacatat tccagcagcc ccaccagaaa ccgcgctcat
     9841 tgcgtgagta ccagtgggtc agcaagcggg cacacggtgg ccatccccag ccctgaaaaa
     9901 aaccgttcag tgtccagacc acaagcagaa gcgtaagtga ttcgccaaag gcaaacacca
     9961 cgttcaataa cccggtggcg aacaagccga cgcccataaa accgcgttgt ccgtggctgt
    10021 catgccacag tccggcggta aatttcgaca atccgtagct gagataaaac agcgaaccca
    10081 gcaggccaat gtcgccttta tccagtccga ggtcggtttg tagcgccgga aggacgtaat
    10141 tcacgctttt tcgcgtcaga taaaatgcgg cgtagccaat aatcatgcac atcagcaatc
    10201 gcggacgtaa tgtccggtag cattgatcaa tttctctcgc tgaacgcgcg tgcatggttg
    10261 tctccatgtt gctgactata tgaaaatgaa agtgaaaggg gatgagagaa aatccgggat
    10321 acctaagact ttttcctggt tatccggcga tttgttgcaa aaatgtgggc aagttaacaa
    10381 ttacccgcgt gccgtgctgt ttttcgagcg ttaagtcgcc gcccagggcg ctgacgcgct
    10441 cacgcatccc ctgaatgcca aatccggcca ttttcccgct ggcgatcccc acaccgttgt
    10501 cgctgacttc cagatgcaat acctctccct gctggcgcag gataatcgtc acttcgctgg
    10561 cttcggcgtg tttacagatg ttattgagca actcctgcaa taaccgatac aaggtaaagc
    10621 gcaccgtttc gttttctggt gtggagttca gttgataggc aaactggcaa tgaatgccgc
    10681 gttcactaaa ggcaaattca ttaatcagat gcagcaacgc ttcgcgaaat gtcagttcat
    10741 caagcgcggg aggccgcaac tggcgcagca attggcgggt ggagagatga atgcggcggg
    10801 cgagctcatt gatttggctg gctgcggatt gaatttgcgc cggatcacgc gcccgcttca
    10861 ccagttgcga ctggatctga atggcggtga tgttctgacc gatttcgtcg tgcagctctc
    10921 gcgccaggtt ttttcgcgtg tcctcctcgg tgtggatcag cttttccgcc aatgcccgcc
    10981 gcgctgccag ctcggcctcc agccgttgtc ggtaatggtg caggtttagc gccagatgtt
    11041 gctggcgact gatggcaatg cccaggccaa ttcccagcaa ggcctgggtc gcgaggaaaa
    11101 tctccagctc caccaggtta ctaaagccca cgccaatctg gcgggcgatg gtgatcatca
    11161 tgctgcccaa aagcccggag agtacgccac cttgccagcc aaatttccac gccatcacca
    11221 cattgggcaa aaagaccacg atcaacagca gacgttcaat ttcaggtgac agcaccatct
    11281 gagtgccgat accaataata aaaaacaggc tacaccagat gattaaagag gtacgcaagg
    11341 ggggattatg cgtatcgagt cccaggagct ggtagcgatg ttgctggcgc agaaactcaa
    11401 agataagata gacaaacggc gtcagtaata cgccgccggt aaatgaggtc aaacccggca
    11461 gcattattgg gcctgacata aaaggggaga gcagggcggt attcaacaat gaagctgccg
    11521 tgacagtggc taacagcaac gtcagacgct gccagtaaag tggaaaacgg tgccagaagt
    11581 tttgcgcgag gaggacagga aacaggctaa taaccggcgt tgccagcatg agataaccgt
    11641 tcaatagctg ttcactatgg agccagaaca tcatcagcaa aggggggagc accaacgcag
    11701 gccagtagcg gcgagagagc aaaataacaa gggcgagata aactccctgc gggagaaaca
    11761 acaccgcctg ttgaccgtta ttggtcagat aaaagctgag cgtccacagc atcagccagc
    11821 cggttcccca ggccagcaca ataaacagcg aaatcaccca atgacgaaga ttgcgcgaca
    11881 ttaatgcccc gccagcagat tgtggtcgag cgcaaaatgt accagttcaa tggtgctatg
    11941 gcagttcagt tttcccagca cattggcgcg atgcacatgt actgttttat gactgagttc
    12001 gagtttaaag gcgatctctt taacgctgtc tcctttgacc agtaaatcaa acacttcacg
    12061 ttctcgtggg gtgagaattt ccagcgcctg ggcgggttgc ccgccgccgc gtaaagcccg
    12121 aatcgcatcg gcacagagat aatgcccgcc cagcccgacc gagcgcaccg cctgcactaa
    12181 ctcttccggc ccacagcgtt tggttaaata cccgctggca ccggcatcca gcgcgctttg
    12241 cacaaaggtt ggcgcgtcat aaatgctaag aataatggcg cgaaattgcg gtttttgtgc
    12301 acgcaggcgc ttaagcaaac tgaggccgtt ttcgtccggc atggcaatat caatgacggc
    12361 aacattgacg tcatcacgga ttaatgccga ccatgcctgc gcggcactac tgtactgacc
    12421 aatgacctcc agatcgtctt ccagagagag taattgtgcg aagccagagc gcaccaccac
    12481 atggtcatcc accagcacta cccgaatcat acttttcttc tcttattatc taattctcct
    12541 atcatgcatc acggcgcggc gctggcaatc aaccagcgac agatatggaa tggatgtaac
    12601 aaaatggcaa taatttgccg gagcgcgcac atcttatctg gcttgtgaac cggcgggata
    12661 cgttcgccaa aaccgcaaaa tgcgcaaact cgctaaccat tgccgatttt tttctaaaaa
    12721 cgctttgttc ttaacaagac gttttaattc ctttatcaaa ctgacccgca acgaaatact
    12781 gctcccataa caagacaggg gagcaggcaa tcatggcaat ttcatcgcgt aacacacttc
    12841 ttgccgcact ggcattcatc gcttttcagg cacaggcggt gaacgtcacc gtggcgtatc
    12901 aaacctcagc cgaaccggcg aaagtggctc aggccgacaa cacctttgct aaagaaagcg
    12961 gagcaaccgt ggactggcgt aagtttgaca gcggagccag catcgtgcgg gcgctggctt
    13021 ctggcgacgt gcaaatcggc aacctcggtt ccagcccatt agcggttgca gccagccaac
    13081 aggtgccgat tgaagtcttc ttgctggcgt caaaactggg taactccgaa gcgctggtgg
    13141 taaagaaaac tatcagcaaa cccgaagatt tgattggcaa acgcattgcc gtaccgttta
    13201 tctccaccac ccactacagc ctgctggcgg cgctgaaaca ctggggcatt aaacccgggc
    13261 aagtggagat tgtgaacctg cagccgcccg cgattatcgc tgcctggcag cggggagata
    13321 ttgatggtgc ttatgtctgg gcaccggcgg ttaacgccct ggaaaaagac ggcaaggtgt
    13381 tgaccgattc tgaacaggtc gggcagtggg gtgcgccaac gctggacgtc tgggtggtgc
    13441 gcaaagattt tgccgagaaa catcctgagg tcgtgaaagc gttcgctaaa agcgccatcg
    13501 atgctcagca accgtacatt gctaacccag acgcgtggct gaaacagccg gaaaacatca
    13561 gcaaactggc gcgtttaagc ggcgtgccgg aaggtgacgt tccggggctg gtgaagggga
    13621 atacctatct gacgccgcag caacaaacgg cagaactgac cggaccggtg aacaaagcaa
    13681 tcatcgacac cgcgcagttt ttgaaagagc agggcaaggt cccggctgta gcgaatgatt
    13741 acagccagta cgttacctcg cgcttcgtgc aataaaagga ggcgcagatg ctgcaaatct
    13801 ctcatctcta tgccgattat ggcggcaaac cggcactgga agatatcaac ctgacgctgg
    13861 aaagcggcga gctactggtg gtgctggggc cgtccggctg cggtaaaacc accctgctga
    13921 atctgattgc cggttttgtg ccttatcagc atggcagcat tcaactggcg ggtaagcgta
    13981 ttgagggacc gggagcagag cgcggggtgg tttttcagaa tgaagggctg ctaccgtggc
    14041 gcaatgtaca ggacaacgtg gcgttcggcc tgcaattggc aggtatagag aaaatgcagc
    14101 gactggaaat cgcgcaccag atgctgaaaa aagtcggcct tgaaggggcg gaaaaacgct
    14161 atatctggca gctttccggt ggtcaacgtc agcgggtggg gattgcccgc gcgctggcgg
    14221 cgaatcccca gctgttatta ctcgacgaac cgtttggtgc gctggacgcc ttcacccgcg
    14281 accagatgca aaccctgctg ctgaaactct ggcaggagac gggcaagcag gtgctgttga
    14341 ttacccacga tatagaagaa gcggtgttta tggcgactga actggttctg ctttcacccg
    14401 gccctggccg cgtgctggag cggctgccgc tcaactttgc tcgccgcttt gttgcgggag
    14461 agtcgagccg cagcatcaag tccgatccac aattcatcgc catgcgcgaa tatgttttaa
    14521 gccgcgtatt tgagcaacgg gaggcgttct catgagtgtg ctcattaatg aaaaactgca
    14581 ttcgcggcgg ctgaaatggc gctggccgct ctcgcgtcag gtgaccttaa gcattggcac
    14641 gttagcggtt ttactcactg tatggtggac ggtggcggcg ctgcaactga ttagcccgct
    14701 gtttttgccg ccgccgcaac aagtactggc aaaactgctc accattgccg gaccgcaggg
    14761 atttatggac gccacgctgt ggcagcatct ggcagccagc ctgacgcgca ttgtgctggc
    14821 gctactcgcg gcggtgttga tcggcattcc ggtcgggatc gccatggggc taagcccgac
    14881 ggtgcgcggc attctggatc cggtaatcga actttatcgt ccggtgccgc cgctggctta
    14941 tttaccactg atggtgatct ggtttggtat tggcgagacg tcgaagatct tactgatcta
    15001 tttagcgatt tttgcgccgg tggcgatgtc ggcgctggca ggggtgaaaa gcgcgcagca
    15061 ggttcgcatt cgtgccgccc agtcgctggg tgccagccgt gcgcaggtgc tgtggtttgt
    15121 cattttgccc ggcgcgctgc cggaaatcct caccggatta cgcattggtc tgggggtggg
    15181 ctggtctacc ctggtagcgg cggagctgat tgccgcgacg cgcggtttag gatttatggt
    15241 tcagtcagcg ggtgaatttc tcgcgactga cgtggtgctg gcggggatcg cggtgattgc
    15301 gattatcgcc tttcttttag aactgggtct gcgcgcgtta cagcgccgcc tgacgccctg
    15361 gcatggagaa gtacaatgag tgaacgtttg agtattaccc cgctggggcc gtatattggc
    15421 gcacaaattt cgggtgccga cctgacgcgc ccgttaagcg ataatcagtt tgaacagctt
    15481 taccatgcgg tgctgcgcca ccaggtggtg tttctgcgcg atcaagccat tacgccgcag
    15541 cagcaacgcg cgctggccca gcgttttggc gaactgcata ttcaccctgt ttaccctcat
    15601 gccgaagggg ttgacgagat catcgtgctg gatacccata acgataatcc gccagataac
    15661 gacaactggc ataccgatgt gacatttatt gaaacgccac ccgcaggggc tattctggca
    15721 gcgaaagagt taccttcgac cggcggcgat acgctctgga ccagcggtat tgcggcctat
    15781 gaggcgctct ctgttccctt ccgccagttg ctgagcgggc tgcgtgcgga gcatgatttt
    15841 cgtaaatcgt tcccggaata caaataccgc aaaaccgagg aggaacatca acgctggcgc
    15901 gaggcggtcg cgaaaaaccc gccgttgcta catccggtgg tgcgaacgca tccggtgagc
    15961 ggtaaacagg cgctgtttgt gaatgaaggc tttactacgc gaattgttga tgtgagcgag
    16021 aaagagagcg aagccttgtt aagttttttg tttgcccata tcaccaaacc ggagtttcag
    16081 gtgcgctggc gctggcaacc aaatgatatt gcgatttggg ataaccgcgt gacccagcac
    16141 tatgccaatg ccgattacct gccacagcga cggataatgc atcgggcgac gattcttggg
    16201 gataaaccgt tttatcgggc ggggtaatac gagagtggac ggtcccctcg cccccttggg
    16261 gagagggtta gggtgagggg gcgttcaccg tactttcaac aggttaactc cccctttctg
    16321 agaggaaaca aaattaacgc agaatcttct tctcagccaa atccagcgca aagtagctga
    16381 aaatcagatc cgcacccgca cgcttaatcg aacctaagct ttcgagcacg actttctctt
    16441 catctatagc acccgccagc gcggcgaact taatcatcgc gtactcaccg ctcacctgat
    16501 acgcgccaat cggcaattca gtacgttcac gcagctcacg cacgatgtcg aggtacgctc
    16561 cagcaggttt aaccatcagg cagtctgcgc cctgggcttc atccagcagt gattcacgaa
    16621 tcgcctcgcg acggttcatt gggttcatct gatagctttt gcggtcgcct tttaatgcgc
    16681 ttccggcagc ttcacggaac gggccataaa aggaggaggc gaacttggtc gaatacgaca
    16741 taatcgccgt atctttaaag cccgcagcgt ccagcgcctg gcgaattgcc tgtacctggc
    16801 cgtccatcgc ggcggaaggg gcgatgaagt ctgcgcctgc agcagctgca accacggctt
    16861 gcttgcctaa attttccaga gtcgcgtcgt tgtcgacgcc atgctcgcac agcacaccgc
    16921 agtgaccgtg agaagtgtat tcacagaaac aagtgtctga catgacgatc atttctggca
    16981 cagtctgctt gcagatgcgc gacatacgcg ccaccaatcc atcttcccgc caggcgtcgc
    17041 tgccggtttc atcggtatgg tgagagatgc cgaaagtcat cacggaacga ataccggcgt
    17101 tggcgatgcg ttcaatttcg cgtgccagat gtttctctgg aatgcgcatc acgcctggca
    17161 tggcttcaac ggctttgtag tcgtcaattt cttcttcaac aaagatcggc aacaccaggt
    17221 cgttaaggct aagtgttgtc tcttcaaaca tagcgcgcag cgcaggagat ttgcgcaggc
    17281 gacgagggcg ttggattaag tctgtcatgg tctgcctgat gtttgtggaa tcgaggggca
    17341 tagtatacct gaagcagggt ggggatgttt tacgaaagtt gtcgcgatgt tgacaagaag
    17401 agaatggaag agaggccagg acatctttgt aatagaaata gcaataat
//
LOCUS       DAPGOF010000101        17402 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.102, whole genome shotgun sequence.
ACCESSION   DAPGOF010000101 DAPGOF010000000
VERSION     DAPGOF010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.102"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            319..1662
                     /locus_tag="RHV13_002958"
     CDS             319..1662
                     /locus_tag="RHV13_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242473.1"
                     /translation="MRPISNLNTSAATYIPPQQLPSSCLETLTLLVQMNNYIKRDADY
                     STGMAVTPLVPEEVHLLAVAMTTELHRHQFQPVVSANDLQLPEPFTFDVAGFTITFTK
                     TQEHDNTGNLVKIAVSKSGVCTSTNIALELFHSIVTTLMSRSQYGTFDLWSVRPILTD
                     ENQNRVHEAARYSPAQQYGREETHFTNNGMREFGNISPLAWRNDVELQQSCNTSNIPP
                     NTANDDINNTRQTIEDQPEADEPQQYVKLTVDDMRKWAAMTQQARNALEGVQGWCTRN
                     HFNIKNARNYLTDHGLNYAGQVKVNGPHEYAKFTLEHIRQWAALDKHVRKPVGYLEKW
                     CKERNLAPTTARNYLKNDGLTALGELKLKGPQKWVTFTFGDIVQWANMSQEERDRAGG
                     AQKWSKKRGFQWSTARSYLKLSGMTVQGAKKLTWLKNSGNISNPFYLAQPTSRRT"
     gene            complement(1811..3319)
                     /gene="putP"
                     /locus_tag="RHV13_002959"
     CDS             complement(1811..3319)
                     /gene="putP"
                     /locus_tag="RHV13_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HDV1242474.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEG"
     gene            complement(3478..3687)
                     /locus_tag="RHV13_002960"
     CDS             complement(3478..3687)
                     /locus_tag="RHV13_002960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242475.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            3742..7704
                     /gene="putA"
                     /locus_tag="RHV13_002961"
     CDS             3742..7704
                     /gene="putA"
                     /locus_tag="RHV13_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HDV1242476.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVADQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGYVREATPC
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGRGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLQMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSTVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(7744..8382)
                     /gene="rutR"
                     /locus_tag="RHV13_002962"
     CDS             complement(7744..8382)
                     /gene="rutR"
                     /locus_tag="RHV13_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HDV1242477.1"
                     /translation="MTQGAVKTTGKRSRTVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            8670..9761
                     /gene="rutA"
                     /locus_tag="RHV13_002963"
     CDS             8670..9761
                     /gene="rutA"
                     /locus_tag="RHV13_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HDV1242478.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGSGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPVLTQEVA"
     gene            9761..10453
                     /gene="rutB"
                     /locus_tag="RHV13_002964"
     CDS             9761..10453
                     /gene="rutB"
                     /locus_tag="RHV13_002964"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HDV1242479.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMMIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            10465..10851
                     /gene="rutC"
                     /locus_tag="RHV13_002965"
     CDS             10465..10851
                     /gene="rutC"
                     /locus_tag="RHV13_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HDV1242480.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINETYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            10859..11595
                     /gene="rutD"
                     /locus_tag="RHV13_002966"
                     /pseudo
     CDS             10859..11595
                     /gene="rutD"
                     /locus_tag="RHV13_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309282.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
     gene            11605..12195
                     /locus_tag="RHV13_002967"
     CDS             11605..12195
                     /locus_tag="RHV13_002967"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HDV1242481.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIIACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            12206..12700
                     /gene="rutF"
                     /locus_tag="RHV13_002968"
     CDS             12206..12700
                     /gene="rutF"
                     /locus_tag="RHV13_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HDV1242482.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            12721..14049
                     /gene="rutG"
                     /locus_tag="RHV13_002969"
     CDS             12721..14049
                     /gene="rutG"
                     /locus_tag="RHV13_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HDV1242483.1"
                     /translation="MAMFGLPHWQLKSTSTESGVVAPDERLPFAQTAIMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     HGINPNISIALGGIIACGLVYTVIGLVVVKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(14307..14480)
                     /locus_tag="RHV13_002970"
     CDS             complement(14307..14480)
                     /locus_tag="RHV13_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HDV1242484.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            14853..15449
                     /gene="wrbA"
                     /locus_tag="RHV13_002971"
     CDS             14853..15449
                     /gene="wrbA"
                     /locus_tag="RHV13_002971"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HDV1242485.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            15470..15697
                     /locus_tag="RHV13_002972"
     CDS             15470..15697
                     /locus_tag="RHV13_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HDV1242486.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(15735..16976)
                     /gene="agp"
                     /locus_tag="RHV13_002973"
     CDS             complement(15735..16976)
                     /gene="agp"
                     /locus_tag="RHV13_002973"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HDV1242487.1"
                     /translation="MNKTLITATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADVLTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
BASE COUNT         4140 a         4602 c         4583 g         4077 t
ORIGIN      
        1 attcacgagt gattgtaact acgtacttta tgaaacacag gaagaaatta ttatttaccg
       61 cgatagttca aggcgtaaaa aaaataataa tattcatgaa actcatcccg atggcagcat
      121 tgtaaattcg ccaataactg gcattcctcc tgctttttac gggtgtttcc attaacacca
      181 gaatgagcgg attgtttact acttcaagaa cgcatccgaa gtatacatcc tggtgcgcag
      241 actgaatcgc ccggagccat gcggatggct acgacttaat acgaatcatt aaccctgtaa
      301 ggatacggag agcatgacat gagaccaata tccaacctca atacgagcgc agcaacctat
      361 attccaccgc agcaactccc gagtagttgt ctggaaactt tgacgcttct ggtgcagatg
      421 aataattata tcaaaaggga tgcagactac agcacgggca tggctgttac tccactggtt
      481 cctgaagagg ttcacttgct ggctgttgcc atgacaaccg aactacatcg acatcagttc
      541 caaccggtgg taagtgcaaa tgatctgcaa cttcctgaac cgttcacctt cgatgttgcc
      601 ggtttcacca tcaccttcac gaagacgcaa gagcatgaca acacgggcaa tctcgtgaaa
      661 attgcagtca gcaagagcgg tgtgtgcacc agtacaaata ttgctcttga gctgtttcac
      721 tccattgtca caacactgat gtcacgcagt caatatggaa cctttgatct ctggtccgta
      781 cggccaatac tcacggatga aaatcaaaac agggtgcatg aagcagctcg ctactccccg
      841 gcccagcaat acggaagaga agaaacacac tttaccaaca atggcatgcg agaatttgga
      901 aatatttctc cgctggcgtg gcgtaacgat gtggaattac agcaatcgtg taatacaagc
      961 aacatcccac caaatactgc aaatgacgac ataaacaaca ccaggcagac catcgaggac
     1021 cagccagagg cggacgagcc acagcaatat gttaaattga ctgtagacga catgcgtaaa
     1081 tgggcggcta tgacccagca ggcgcgaaac gctctagagg gagttcaagg atggtgtacg
     1141 cgcaatcatt ttaatatcaa aaatgccagg aattacctga ccgatcacgg cctgaactac
     1201 gcgggacagg taaaggtaaa cgggccgcat gaatatgcca aattcaccct tgaacatatc
     1261 cgccagtggg cagccttaga caagcatgtg cgaaaaccgg ttggttattt agaaaagtgg
     1321 tgtaaggaaa gaaacctcgc gccaactact gccaggaatt acctgaagaa cgacgggctt
     1381 accgccctgg gtgagttgaa gctgaaaggg ccgcagaaat gggtaacttt caccttcggg
     1441 gacatcgtgc aatgggcgaa tatgagccag gaggagcgtg accgcgcagg gggagcccaa
     1501 aaatggagta aaaaacgcgg cttccagtgg tctactgcca gatcctacct gaaattgagc
     1561 ggaatgacgg tgcaaggcgc gaaaaaactg acgtggctga aaaacagcgg gaatatatcg
     1621 aatccattct atctcgccca accaaccagc cgcaggacat aagctgaagg taccgatagc
     1681 ggcaattttg ttcatctgtg cctggtgggc aaatagcaat tgcccccgaa cactccctgc
     1741 caggcaactt ataccgcatg gcagggaaca ttcgggttaa aaagcaaaaa ataaataatt
     1801 actcttcagc ttagccttcc tgcaaccgtg acggcggagc cgaatgatag tgcgcatcgg
     1861 cctcggcaaa gcgtttttgc atcgccgctg acggcgcttt acccagcaaa ctaaacacta
     1921 caatcccaat actgccaaag ataaagcccg gaataatttc gtacagtccc agccagccga
     1981 actgtttcca gacgataacc gtcagcgccc cgatgatcat ccccgccagc gcaccgttgc
     2041 gcgtcatgcg tgaccacatc accgagaaca gcaccaccgg accaaacgcc gcgccaaagc
     2101 ctgcccacgc gtagctcact aagcccagca cgcggttttc cgggtttgcc gccagcgcaa
     2161 tggccaccag cgccaccacc agcaccatca cacgccctac ccacaccagc tctttctggc
     2221 tggcatgttt acgcagaaac gctttgtata aatcttcggt aatcgcactg gagcacacca
     2281 gcagttggca acttaaggtt gacattaccg ccgccagaat tgccgacagc agaatcccgg
     2341 caatccacgg gttaaacaga atttgcgcca gttcgataaa cacgcgttca gcgttctggt
     2401 ttaccgcacc tgctaccgcc gggtgctcgt taaagtaagc gataccaaag aagccgacag
     2461 ccaccgcccc tgccaggcag aggatcatcc aggtcatact gatacgacgc gcatggacaa
     2521 tgctgtggtg agaatccgcc gccataaaac gcgccaggat gtgcggttgc ccgaagtaac
     2581 ccagccccca tcccatcagc gagataatgg caacaaagtt cagccctttg agcatatcga
     2641 cgttttcgat gctcttttgt ttgatcactt ccagcgagtc accaaagcca ccgacactga
     2701 taatgacgat aaccggcgtc aggatcaggg caaaaatcat caggctggcc tgtacagtgt
     2761 cagtccagct caccgcgagg aaaccgccaa taaaggtgta aaggatcgtc gccgcagcgc
     2821 ccgcccacag agccgtttcg tagctcatgc caaaggtact ttcaaacaga cgcgcgcctg
     2881 ccacaatgcc cgaagcgcaa taaatggtga agaacagcaa aatcaccagc gcggagataa
     2941 tgcgcaaaat gcggctttta tcttcaaaac gcccggtgaa ataatccggt aatgttaagg
     3001 cgttattgtt gtattcggta tgcacacgca accgtccggc caccagcttc cagttaatcc
     3061 acgcgcctaa tgtcaggcca atggcgatcc aactttcgga aatcccggaa agaaaaaccg
     3121 cgcccggcaa ccccattaac agccagccgc tcatatccga tgcacccgcc gataatgccg
     3181 tcacgaatgg cccaaggcta cgtccgccca gaatatagtc gtcaaagttt ttcgttgatc
     3241 gccaggcgat aaacccaatc aatatcatgc caaagatata gacacaaaat gtcaccaaca
     3301 tcggtgtgct aatagccatc taaagtctcc aaaaaattat tatcggcaat gtcgaaactt
     3361 gccgttatat ctgccaccgg aacggggtaa cagagtttat gttttaccag ggcgaacgta
     3421 tcctgccgga agcgctggtt attcacaatc gatttaacac accatttaca ttaaatttta
     3481 gtgctcagcg acactatttt tcatcaggtt gcactctctc acattttttg cggttgcacc
     3541 tttcaaaaat gttaactgcc gcagagaaaa agtctgagtt atttttttaa tccctgtcat
     3601 atcgatttct tttattaaca tttcattcat ttttaagctt gctacgcagg tcacatttaa
     3661 catggttgca caaagttgca acatcatgga tatttcacga taacgttaag ttgcaccttt
     3721 cagaacaaca ggagtaatgg catgggaacc accaccatgg gggttaagct ggacgacgcg
     3781 acgcgtgagc gtattaagtc tgccgcgaca cgtatcgatc gcacaccaca ctggttaatt
     3841 aagcaggcga ttttttctta tcttgaacaa ctggaaaaca gcgatactct gccagagcta
     3901 cctgcgctgc tttctggcgc tgccaatgag agcgatgaag caccgactcc ggcagaggaa
     3961 ccacaccagc cattcctcga ctttgccgag caaattttgc cccagtcggt ttcccgcgcc
     4021 gcgatcaccg cggcctatcg ccgcccggaa accgaagcgg tttctatgct gctggaacaa
     4081 gcccgcctgc cgcagccagt tgctgatcag gcacacaaac tggcgtatca actggccgat
     4141 aaactgcgta atcaaaaaaa tgccagtggc cgcgcaggta tggtccaggg gttattgcag
     4201 gagttttcgc tgtcatcgca ggaaggcgtg gcgctgatgt gtctggcaga agcgttgttg
     4261 cgtattcccg acaaagccac ccgcgacgcg ttaattcgcg acaaaatcag caacggtaac
     4321 tggcagtcac acattggtcg cagcccgtcc ctgtttgtta atgccgccac ctgggggttg
     4381 ttgtttactg gcaaactggt ttccacccat aacgaagcca gcctctcccg ctcgctgaac
     4441 cgcattatcg gtaaaagcgg tgaaccgctg atccgcaaag gtgtggatat ggcgatgcgc
     4501 ctgatgggcg agcagttcgt cacgggcgaa accatcgcgg aagcgttagc caatgcccgc
     4561 aagctggaag agaaaggttt ccgttactct tacgatatgc ttggcgaagc tgcactaact
     4621 gccgcagatg cacaagcgta tatggtttcc tatcagcagg cgattcacgc cattggtaaa
     4681 gcgtccaatg gtcgtggcat ctatgaaggt ccgggcattt caatcaagct gtcggcgctg
     4741 catccgcgtt acagccgcgc ccagtatgac cgggtaatgg aagagcttta cccgcgtctg
     4801 aaatcactca ccctgctggc gcgtcagtac gatattggta tcaacattga cgccgaagag
     4861 gccgatcgtc tggagatctc cctcgatctg ctggaaaaac tctgtttcga gccggaactg
     4921 gcaggctgga acggcatcgg ttttgttatt caggcatatc aaaaacgctg cccgttggtg
     4981 atcgattacc tgattgatct cgccacccgc agccgtcgcc gtctgatgat tcgcctggtg
     5041 aaaggcgcgt actgggatag tgaaatcaag cgtgcgcaga tggacggcct tgaaggttat
     5101 ccggtttata cccgcaaggt gtataccgac gtttcttatc tcgcctgtgc gaaaaagctg
     5161 ctggcggtgc cgaatctaat ctacccgcag ttcgcgacgc acaacgccca tacgctggca
     5221 gcgatttatc aactggcagg tcaaaactac tacccgggtc agtacgagtt ccagtgcctg
     5281 catggtatgg gcgagccact gtatgagcag gtcaccggga aagttgccga cggcaaactt
     5341 aaccgtccgt gtcgtattta tgctccggtt ggcacacatg aaacactgct agcgtatctg
     5401 gtgcgtcgcc tgctggaaaa cggtgctaac acctcgtttg ttaaccgtat tgccgacacc
     5461 tctttgccac tggatgaact ggtcgccgat ccggtcactg ctgtagaaaa actggcgcaa
     5521 caggaagggc aaactggatt accgcatccg aaaattcccc tgccgcgcga tctttatggt
     5581 cacgggcgcg acaactcggc agggctggat ctcgctaacg aacaccgtct ggcctcgctc
     5641 tcttctgccc tgctcaatag tgcactgcaa aaatggcagg ccttgccaat gctggaacaa
     5701 ccggtagcgg caggtgagat gtcgcccgtt attaaccctg cggaaccgaa agatattgtg
     5761 ggctatgtgc gtgaagccac gccgtgtgaa gtagaacagg cgctggaaag tgcggttaat
     5821 aacgcgccaa tctggtttgc cactcctccg gctgaacgcg cggcaatttt acatcgcgct
     5881 gccgtgctaa tggaaagcca gatgcagcaa ctgattggta ttctggtgcg tgaagccgga
     5941 aaaaccttca gtaacgccat tgccgaagtg cgcgaagcgg tcgattttct ccactactac
     6001 gccggacagg tgcgggatga tttcgctaac gaaacccacc gtccattagg gcctgtggtg
     6061 tgtatcagcc cgtggaactt cccgctggcg attttcaccg ggcagattgc tgccgcactg
     6121 gcggcaggta acagcgtgct ggcaaaaccg gcagaacaaa cgccgctgat tgccgcgcaa
     6181 gggatcgcca ttttgctgga agcgggtgta ccgccaggcg tggtgcaatt gctgccaggt
     6241 cggggggaaa ccgtgggcgc gcaactgacg ggtgatgatc gcgtgcgcgg ggtgatgttt
     6301 accggttcaa ccgaagtcgc tacgttactg cagcgcaata tcgccagccg cctggacgct
     6361 cagggtcgcc ctattccgct catcgctgaa accggcggca tgaacgcgat gattgtcgat
     6421 tcttcagcac tgaccgaaca agtcgtaata gatgtgctgg cctcggcgtt cgacagtgcg
     6481 ggccagcgtt gttcggcgct gcgcgtgctg tgcctgcaag atgagattgc cgaccacacg
     6541 ttgcaaatgc tgcgcggcgc aatggccgaa tgtcggatgg gtaatccggg tcgcctgacc
     6601 accgatatcg gtccggtgat tgatagcgaa gcgaaagcca atattgagcg ccatattcag
     6661 accatgcgta gcaaaggccg tccggtgttc caggcggtgc gggaaaacag cgaagatgcc
     6721 cgtgaatggc aaagcggcac ctttgtcgcc ccgacgctga tcgaactgga tgactttgcc
     6781 gaattgcaaa aagaggtctt tggtccggtg ctgcatgtgg tgcgttacaa ccgtaaccag
     6841 ctaccggagc tgatcgagca gattaacgct tccggctatg gtctgacgct tggcgttcat
     6901 acgcgtattg atgaaaccat cgcccaggtc actggctcag cccatgttgg taacctgtat
     6961 gttaaccgta atatggtggg cgcagtggtt ggggtgcagc cattcggcgg cgaagggttg
     7021 tccggtaccg ggccgaaagc aggcggtccg ctctatctct accgtctgct ggcgaatcgc
     7081 ccggaaagtg cgctggcggt gacactcgcg cgtcaggatg cagagtatcc ggtcgatgcg
     7141 cagttgaaag ccgcattgac tcagccgcta aatgcactgc gggaatgggc agcaaatcgt
     7201 ccagaattgc aggcgttatg tacgcaatat ggcgagctgg cgcaggcagg aacacaacga
     7261 ttgctgccag ggccgacggg tgagcgcaac acctggacgc tgctgccgcg tgagcgcgta
     7321 ttgtgtatcg cggatgatga acaggatgcg ctgactcagc tcgccgccgt gctggcggtg
     7381 ggcagccagg tgctgtggcc agatgacgcg ctgcatcgtc agttagtgaa ggcattgcca
     7441 tcgactgtca gcgagcgtat tcaactggcg aaagcggaaa atattaccgc tcaaccgttt
     7501 gatgcggtga tcttccacgg tgattcggat cagcttcgcg cattgtgtga agcagttgcc
     7561 gcgcgggatg gcgcaattgt ttcggtgcag ggttttgccc gtggcgaaag caatatcctt
     7621 ctggaacggc tgtatatcga gcgttcgctg agtgtgaata ccgctgccgc tggcggtaac
     7681 gccagtttaa tgacgatcgg ttaaacaggt agccggagga tgttacacct tcctccggca
     7741 tcattagcgt ggtcgaatcc cctcaataat aatccgctgc acgttttcaa ccgtttgatt
     7801 gaaaaatacc tcatcgcgca acgttgcgcc tgtcactgcc tccacctgag gggcgaaatc
     7861 ggcgtaatgt tgagtggaag cccaaatcat aaaaatcaaa tgctgcggat caattggcgc
     7921 gagtttgccg cttttgaccc aaccggcaat cagcgccgat ttctcatcaa ttaatgcttt
     7981 caaatcgccc gtcagttcat ccattaacaa cggcgcacct gccagcattt ccatacagaa
     8041 caggcgcgaa gcctgcggat aatcgcggga gacttccagc ttcagacgga tgtactcttt
     8101 gatcgccgcc agcggggcga aatcttcacg aaacgctttt aacggagcca gccagatatc
     8161 gagaatctgc cgcagcacgg caatatacag cgcctctttt gacggaaagt aatacagcag
     8221 attggttttt gaaacacccg ccaactctgc gatctgctcc agccgtgtgc cgtgaaaacc
     8281 gaattgtgaa aaagtgtcca gtgctgcgct aagaatcgct tttttcttcg cgcttactgt
     8341 acgcgaacgt ttacccgttg ttttgactgc gccttgcgtc atgcgctctc ccctcttgta
     8401 gattggcaac aggatagcaa aacccccgcc cggcctcgac cttctgcact ctcatcgcgc
     8461 acggatgcat gttttatgtg caactgtttt gaccgtttag tccacttttt tccagatatt
     8521 ttatataacc caacattaac acattaataa cattcacttt ttaaaaactg gcatccgctt
     8581 tgcaaacaag ccattacgcg gctgaaaagg atatgcagga tgcagcgccc cgtctgactt
     8641 tcactttacg agacgaagag aggttgatga tgaaaattgg cgtattcgta cctattggca
     8701 acaacggctg gctcatttcg acccacgcgc cgcagtacat gccgaccttt gaactgaata
     8761 aagccattgt gcaaaaagcg gagcactacc atttcgattt cgccctgtcg atgatcaaac
     8821 tgcgtggctt tggcggcaaa actgagttct gggatcacaa ccttgagtcg ttcaccttga
     8881 tggcggggct ggcagccgtg acctcgcgca ttcagattta cgccactgcc gccaccttaa
     8941 cgttacctcc ggcaatcgtc gcccgtatgg cggcaaccat cgactccatc tctggcgggc
     9001 gttttggcgt caacctcgtg actggctggc aaaagcccga gtatgagcag atgggtatct
     9061 ggcctggcga tgactatttc tcccgtcgtt acgactatct caccgaatat gttcaggtgc
     9121 tgcgcgacct gtggggctcg gggaaaagtg attttaaagg cgattttttc accatgaatg
     9181 attgtcgcgt cagtccgcaa ccgagtgtac ccatgaaagt gatctgcgcc gggcaaagcg
     9241 acgctggcat ggcgttctcc gcccagtatg ccgatttcaa cttctgtttc ggcaaaggcg
     9301 taaatacacc cacggctttc gccccgaccg ctgcgcggat gaaacaggcc gcagagcaaa
     9361 ccgggcgcga cgttggctct tatgtattgt ttatggtgat tgccgatgaa accgacgatg
     9421 ccgctcgcgc caaatgggaa cactacaaag cgggcgcgga tgaagaggcg ttaagctggc
     9481 taaccgaaca aagtcagaaa gatacccgct ccggtactga caccaacgtc cgtcagatgg
     9541 cagatcccac ttcggcggta aacatcaata tggggacgtt agtcggttct tacgccagtg
     9601 tcgcgcgcat gttagatgaa gtcgcaagcg tgcctggtgc cgaaggcgtg ctgttaacct
     9661 tcgacgattt tctgtcggga atcgaaacct tcggcgagcg cattcaacca ctgatgcagt
     9721 gccgcgccca tctccctgtg ctgactcagg aggtggcatg atgacgacat taaccgctcg
     9781 accggaagcc attaccttcg atccgcagca aagtgcgctg atcgtggtgg atatgcaaaa
     9841 cgcctatgcc acgccaggcg gctacttaga tctcgccggg tttgatgtct caaccactcg
     9901 cccggtcatt gccaacattc aaaccgccgt gaccgcagcg cgagcggcag ggatgatgat
     9961 catctggttt caaaatggct gggatgaaca gtatgtcgag gctggcggcc ccggctcacc
    10021 gaattttcat aaatcgaacg ccttgaaaac catgcgtaag cagccgcagc tacaggggaa
    10081 attgctggcg aaaggctcct gggattatca actggtggat gaactggtgc cgcagcctgg
    10141 cgatattgtg ctgccgaagc cgcgctacag cggtttcttc aatacgccgc tggacagcat
    10201 tttgcgcagc cgtggaatac gccatctggt tttcaccggt atcgctacca acgtctgcgt
    10261 cgaatcgacg ctacgcgatg gcttttttct ggagtatttc ggcgtggtgc tggaagacgc
    10321 aacacaccag gcggggccgg aatttgcaca gaaagccgcg ttgttcaata tcgaaacctt
    10381 ttttggctgg gtcagcgacg tcgaaacgtt ctgcgacgcg ctttctccca cgtcctttgc
    10441 tcgtatcgct taaggagttt aacgatgcca aaatccgtaa ttattcccgc tggcagcagc
    10501 gcaccgctgg cccccttcgt tcccggcacg ctggctgatg gcgtggtgta tgtctccggt
    10561 acgctggctt ttgatcaaca taataacgtg ctgtttgccg atgacccaaa ggcgcaaacc
    10621 cgccacgttc tggaaaccat ccgcaaggtg atcgagacgg cgggtggcac gatggcggat
    10681 gtgaccttca acagcatctt tattaccgac tggaaaaatt acgccgcgat taacgaaacc
    10741 tacgccgagt tttttccagg tgataaaccg gcgcgattct gcattcagtg cggactggta
    10801 aaacctgacg cgctggtgga aatcgccaca attgcgcata tcgccaagtg aggccgcgat
    10861 gaaactttca ctctcacctc ccccttatgc tgatgcaccc gtagtggtgt tgatttcggg
    10921 tcttggcggt agcggcagtt actggttacc gcaactggcg gtgctggagc aggagtatca
    10981 ggtagtctgt tacgaccagc gcggcaccgg caataatccc gacacactgg cagaagatta
    11041 cagtatcgcc cagatggcag cggaactgca tcaggcgctg gtagccgcag ggattgagca
    11101 ttacgcagtg gtcggccatg cgctcggtgc gctggtggga atgcagctgg cgctggatta
    11161 tcccgcgtcg gtaactgtgc tggtctgcgt taacggctgg ctacgaataa acgcccatac
    11221 gcgccgctgt tttcaggttc gcgaacgatt actgtatagc ggtggcgcgc aggcatgggt
    11281 ggaagcgcag ccgttgttcc tctatcccgc cgactggatg gcggcccgcg cacctcgcct
    11341 tgaggcagaa gacgcgctga ctttagtcac catgcggatc gcatccgctg cccggtgcaa
    11401 atcatctgcg ccagtgatga tctgctggtg ccatcagcat gttccagtga acttcatgcc
    11461 gccctgcccg atagccagaa aatggtgatg cgctatggcg gacacgcctg caacgtgacc
    11521 gatcccgaaa cgtttaatgc tctgttactc aacgggcttg ccagcctgtt acatcaccgt
    11581 gaagccgccc tgtaaggaat tgctatgaac gaagccgtta gcccaggtgc gcttagcacc
    11641 ctgttcaccg atgcccgcac tcacaacggc tggcgggaga cacccgtcag cgatgagacg
    11701 ttacgggagc tttatgccct gatgaaatgg gggccgacat cagctaactg ttctccggca
    11761 cgtatcgtgt ttatccgcac tgcagaagga aaagaacgtc tgcgcccggc gctttccagc
    11821 ggcaatctgc aaaaaaccct gaccgcgccc gtcaccgcta tcgtcgcatg ggacagtgaa
    11881 ttttatgaac ggttaccgca actgtttcct cacggcgatg cccgcagttg gtttacctcc
    11941 agcccacaac ttgccgaaga aacagcgttt cgcaacagtt ccatgcaggc ggcttatctt
    12001 atcatcgcct gccgtgcgct gggactggat accggcccga tgtcgggttt tgaccgtcaa
    12061 cacgttgacg acgccttttt tgcgggcagc acgctgaaga gcaatctgct gattaatatc
    12121 ggctatggcg atagcagcaa actttttgcg cgcctgccac gtctgtcctt tgaagaagcc
    12181 tgcgggctgt tgtaaggagc aaaccatgaa cattgtcgat caacaaacct ttcgcgatgc
    12241 gatgtcctgt atgggcgcgg cagtcaatat catcaccacg gatggtccag ccgggcgcgc
    12301 cgggttcacc gccagcgcgg tctgcagcgt gaccgacacg ccgcctacat tactggtgtg
    12361 cctgaatcgt ggggcgtccg tctggccggt attcaatgaa aatcgaacgc tgtgtgtaaa
    12421 tacactgagc gccgggcagg agccgctttc aaatcttttt ggcggcaaaa cgcccatgga
    12481 acaccgcttt gccgccgccc gctggcagac gggtgtgacc ggatgcccgc aactggaaga
    12541 ggctctggtt tcgtttgact gccgtatcag ccaggtggtg agcgtcggca cccacgacat
    12601 tctgttttgc gccatcgaag cgattcatcg tcacgccaca ccctacgggc tggtgtggtt
    12661 cgatcgcagt tatcacgcgc tgatgcgccc tgcttgttaa cctccttaag gagacagctg
    12721 atggcaatgt tcggtcttcc tcactggcag ttaaaatcga cctctacaga gagcggcgtg
    12781 gtcgcgccgg atgaacgatt gccgtttgct cagacggcaa taatgggcgt tcaacacgcg
    12841 gtggcgatgt ttggcgcgac ggtgttaatg ccgattctga tggggctgga tcccaatctt
    12901 tccattttga tgtcggggat cggcacgtta ctgtttttct ttattaccgg cgggcgcgtt
    12961 cccagttatc tcggttccag cgcggctttt gtcggcgtgg tgatcgccgc aacgggcttt
    13021 aacggtcatg gcattaaccc gaatatcagc atcgcgctgg gcgggattat cgcctgtggg
    13081 ctggtttata ccgtcattgg cctggtggtg gtgaaaatcg gcacgcgctg gattgaacgt
    13141 ctgatgccgc ccgtagtgac gggcgcggtg gtgatggcga ttggcctgaa cttagcgcct
    13201 atcgcagtga aaagtgtatc ggcttcggcc tttgatagct ggatggcggt aatgacggtg
    13261 ctgtgtattg ggctggtggc ggtgtttacc cgtggcatga tccagcggtt attgattctt
    13321 gtaggattaa ttgtcgcctg tctgctgtat ggcgtgatga ccaatgtttt agggctgggg
    13381 aaagccgtcg attttactct cgtcagccat gctgcctggt tcggtctgcc acatttttcc
    13441 actcctgctt ttaatggtca ggcaatgatg ctgattgcgc ccgtggcggt gattctggtg
    13501 gcggaaaact taggtcatct caaggcggtc gccgggatga ccggacgcaa tatggatccg
    13561 tatatgggac gggcattcgt cggtgatggg ctggcgacga tgctttctgg ttctgtcggt
    13621 ggcagcggcg taaccaccta cgccgagaat atcggtgtaa tggccgtgac aaaagtctac
    13681 tcgacgctgg tgtttgttgc tgctgccgtc atcgccatgc tgttgggctt ttcaccgaaa
    13741 tttggcgcgc tgattcatac cattccggcc gcggtgattg gtggtgcatc aattgttgtg
    13801 ttcgggctga ttgcagtcgc aggcgcaagg atatgggtac aaaaccgtgt cgatttaagc
    13861 cagaacggta atttgattat ggtcgcagta accttagtgc tgggcgcggg tgattttgct
    13921 ctcacgctgg gcggttttac gttgggaggg atcggtacag ccacctttgg cgcgattttg
    13981 ctcaatgcgt tgttaagtag aaggttggtt gacgttccac cgccagaagt tgttcatcag
    14041 gagccataaa cagaaaaccg ccggggttac ccggcggtgc agattgttga caacgtacgc
    14101 attgttcatg ccggatgcgg cgtaaacgcc ttatccggcc tacaaaacag tgcaaattca
    14161 atatattgca atcttctggt aggcctgata agcatcgcgc atcaggcaag cttacatttg
    14221 tcatcagtat caaccaccgg gtaaccggcg gtgatggctt tttgacgagc tacagacaaa
    14281 aatgcattcg tcattgatta gcccggctag ttgtcgcttt tgccgtgact gctcttgcca
    14341 ccttttttac ctgcttcaga tgcgcgctgc gggtcattct tgaaattacc cccgctgtgc
    14401 tgtccacctt ttttacctgc ttctgatgct ctttcgcggt cttctgcaaa attgccggaa
    14461 ccgcctcgat ggtttgccat tactgacctc cgtctgggta tagacatcat attgcataga
    14521 attaacgcgg cgtatggcgg ctgccatatt ttcgctacgt gggactaagc ttagtgaact
    14581 gacgcgggca gacccgcgtt tagtaatatc ctgcaacagc ttcttagcga gaatagatat
    14641 catctccaat aattatccat aagccgctct tatgattaaa aaaacgcgat gagataaaaa
    14701 tgtgtcattt tgcgacaaaa ttacgtgctt gtgaaagttg ttataaatca aataagtggt
    14761 tgtgaaattt gcactctgaa aaggacgtct tatctttaaa taagtggtag cgaatcgcta
    14821 cggaatagag ataacacgag gagtggttag aaatggctaa agttctggtg ctttattatt
    14881 ccatgtacgg acatattgaa acgatggcac gcgcagtcgc tgagggtgca agcaaagtgg
    14941 atggcgcaga agttgtcgtt aagcgtgtac cggaaaccat gccgccgcaa ttatttgaaa
    15001 aagcaggcgg taaaacgcaa actgcaccgg ttgcaacccc gcaagaactg gccgattacg
    15061 acgccattat ttttggtaca cctacccgct ttggcaacat gtccggtcaa atgcgtacct
    15121 tcctcgacca gacgggcggc ctgtgggctt ccggcgcact atacggaaaa ctggcgagcg
    15181 tctttagttc caccggtact ggcggcggtc aggaacaaac tattacttca acctggacga
    15241 cccttgcgca tcacggcatg gtaattgtcc ccattggcta cgcagcgcag gaattatttg
    15301 acgtttcaca ggttcgcggc ggtacgccgt acggcgcaac caccatcgca ggcggtgacg
    15361 gctcacgcca gccaagccag gaagaactgt ctattgctcg ttatcaaggg gaatatgtcg
    15421 caggtctggc agttaaactt aatggctaat cttcaacagg aggatacgca tgccaactca
    15481 agaagcgaaa gctcatcacg tcggtgaatg ggcatctctg cgcaatacgt cgccggaaat
    15541 agctgaggcc attttcgaag tcgccgggta cgacgaaaaa atggcggaaa agatttggga
    15601 agaaggtagt gatgaagtgt tagtcaaagc ctttgctaag accgataaag attcgctttt
    15661 ttggggcgaa cagaccatcg aacgtaaaaa cgtttaacca gcgactcccc cgtttctcac
    15721 gggggagttt tctgttattt caccgcttca ttcaacacgc tatcaaactt atccatcggg
    15781 cagaaaccgt tggcatctat cgggcaaccg cttaattcca gcgtcacgcg ctgcgcaggt
    15841 gcctgcaggg ttaacacatc ggcattacgt aactgttccg cactctggta cacatactcg
    15901 attttcatca aatcgcgatt ggctttgctg tcatgccaac gctgaaaaac gattttgccg
    15961 ccaatcggcg tgcgttcgtt ctggtcatgc aactgatacg gtttgaaatc cagcgccgtt
    16021 aacagagagg ctatgttgga gtcgtgccca accagcactg tgattttcgg cgcactggtg
    16081 cgatcggtga ccagagcttt gtcgatataa ctgaccagcg gtttcgcaac attgcgcgcc
    16141 acttccggag aggtaaacag gctgtcctgg taaccgtttt tcagcttcga caacaccttc
    16201 cactgctggt cagatttgat ttctccccag gccacctgat ccatcggaaa accttcgtag
    16261 tattgcaaag taaacgcatc taccagcgag ttgccgactt tcagcggccc ggaaacacct
    16321 ggttcttgtt gatacttcgc gctaaaggta tttttgccat ccaccagcga acactgttgt
    16381 ttctctttac aggctgggga atctttatag ttaacgattt tttccagtag ctggtagctg
    16441 tcggtaagct ggagtttgct gagctctttc tccattgccg ccaccgcctg ttcactgaat
    16501 gcggcggaat catcggtgat caccggatta aaggttgggt ccatggtgcc cattttttcc
    16561 tggtgatgca caggaatatc acaccccggg aatgcgccgg taataaagaa ctgtgcagtc
    16621 gcaacggtac gttgcagact attggcatag gcatatacgg tgtccggcgg cgggcattcc
    16681 cccgatttta ccatcccctg ctctgccagc cattcacgca tgtaatggcc catatacact
    16741 tcgagcacgc cacctttggt ggtgagttgc ccaccgggga cgtcccattc gggccattta
    16801 ttcggcgtcg actgctccag cacactgcca ttgttcgcca gcggcgcacg taagttatgg
    16861 cggctcatca tgagcacttg ctgtagctga tagccttccg gtacggtttg tgcctgagca
    16921 tttgaagcga gtaaaactat ccctgccaca gttgcggtga ttagcgtttt gttcattcct
    16981 ggcacctctt ttgttatcaa tcactaaaga gtgtgacaga aatatgactt ttttccggga
    17041 acaggttcgc aaaatggcct gttgaacacc tgcttttcta tatacatctt caaaaacagg
    17101 caaggtttga cgcaaactat gcctgaatag cccctgcctc gcgtacgccg ctgataattg
    17161 cacctgttgc taaacagaat atctgcggcg ttaccaatac agagacaata tttaacaata
    17221 aaaaagtgtg agactaataa caatggtttt tagcatatgc ctttgccatt aaattgcata
    17281 acgatttaaa tagtcaagtt gcgcattcat ttccttattg tttaacttgt aagcctgagc
    17341 aaaaagttca ggagggaggt aaacgtcata atgattgccg gtgtttatta aataaccata
    17401 ct
//
LOCUS       DAPGOF010000102        17317 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.103, whole genome shotgun sequence.
ACCESSION   DAPGOF010000102 DAPGOF010000000
VERSION     DAPGOF010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.103"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..432
                     /gene="ecpR"
                     /locus_tag="RHV13_002974"
                     /gene_synonym="matA"
     CDS             <1..432
                     /gene="ecpR"
                     /locus_tag="RHV13_002974"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HDV1242488.1"
                     /translation="SLEKIRKDFVFINLNTDSLSEFINRDNEWLSAVKGKQVVLIAAR
                     KSEALANYWYYNSNIRGVVYAGLSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIR
                     MTVQGMQPKSIARIENCSVKTVYTHRRNAEAKLYSKIYKLV"
     gene            510..1097
                     /gene="ecpA"
                     /locus_tag="RHV13_002975"
                     /gene_synonym="matB"
     CDS             510..1097
                     /gene="ecpA"
                     /locus_tag="RHV13_002975"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HDV1242489.1"
                     /translation="MKKKVLAIALVTVFTGMGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFTII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1155..1823
                     /gene="ecpB"
                     /locus_tag="RHV13_002976"
                     /gene_synonym="yagY"
     CDS             1155..1823
                     /gene="ecpB"
                     /locus_tag="RHV13_002976"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HDV1242490.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNAKRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            1849..4374
                     /gene="ecpC"
                     /locus_tag="RHV13_002977"
                     /gene_synonym="yagX"
     CDS             1849..4374
                     /gene="ecpC"
                     /locus_tag="RHV13_002977"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HDV1242491.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNLGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSSIGSI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGTFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYVNTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSRLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELSQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            4364..6007
                     /gene="ecpD"
                     /locus_tag="RHV13_002978"
     CDS             4364..6007
                     /gene="ecpD"
                     /locus_tag="RHV13_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HDV1242492.1"
                     /translation="MRVNLLIAMIIFALIWPATALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYTGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            5976..6686
                     /gene="ecpE"
                     /locus_tag="RHV13_002979"
     CDS             5976..6686
                     /gene="ecpE"
                     /locus_tag="RHV13_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HDV1242493.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPQDNRERYYRVSFREVPTRNQTRRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDQVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            6999..7328
                     /locus_tag="RHV13_002980"
     CDS             6999..7328
                     /locus_tag="RHV13_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HDV1242494.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(7575..8189)
                     /locus_tag="RHV13_002981"
     CDS             complement(7575..8189)
                     /locus_tag="RHV13_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HDV1242495.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNAAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            8607..9296
                     /gene="paoA"
                     /locus_tag="RHV13_002982"
     CDS             8607..9296
                     /gene="paoA"
                     /locus_tag="RHV13_002982"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit"
                     /protein_id="HDV1242496.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDFSLTRRDLIKVSAATAATAVVYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRTTLLDALRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAVLKEIQDGIPSHVTVDLVSPPETTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            9293..10249
                     /gene="paoB"
                     /locus_tag="RHV13_002983"
     CDS             9293..10249
                     /gene="paoB"
                     /locus_tag="RHV13_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HDV1242497.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLAAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYAFALVSVAAIIQPDGSGRVALGGVAHKPWRIEAADAQLS
                     QGAQAVYDTLFASAHPTAENTFKLLLAKRTLASVLAEARAQA"
     gene            10246..12444
                     /gene="paoC"
                     /locus_tag="RHV13_002984"
     CDS             10246..12444
                     /gene="paoC"
                     /locus_tag="RHV13_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HDV1242498.1"
                     /translation="MKFDKPAGENPIDQLKVVGRPHDRIDGPLKTTGTARYAYEWHEE
                     APNAAYGYIVGSAIAKGRLTALDTDAAQKAPGVLAVITASNAGALGKGDKNTARLLGG
                     PTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVNQPPEDTPDK
                     NVGDFDGAFTSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRT
                     DLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVKVMLPRPTIP
                     NNTTHRPATLQHLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSELLYAGANRHT
                     GLRLATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDTQVDPADP
                     TRRFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNLLEKSGAR
                     VHLEPNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFPVSAGSGG
                     QWGANTSTSGVYAACVKLREMIASAVGFDPEQSQFADGKITNGTRSAMLHEATAGGRL
                     TAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRILNPKTARS
                     QVIGAMTMGMGAALMEELAVDDRLGYFVNHDMVGYEVPVHADIPKQEVIFLDDTDPIS
                     SPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
     gene            12454..13410
                     /gene="paoD"
                     /locus_tag="RHV13_002985"
     CDS             12454..13410
                     /gene="paoD"
                     /locus_tag="RHV13_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HDV1242499.1"
                     /translation="MSYPLFDKDEHWHKPEQAFLTDDHRTILRFAVEALMSGKGAVLV
                     TLVEIRGGAARPLGAQMVVREDGRYCGFVSGGCVEAAAAFEALEMMGSGRDREIRYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLEAQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNRELPVLQAAREAKPFYLGALGSYRTHTLRLQKLHERGWSREETAQ
                     IRAPVGIFPKARDAHTLALSVLAEVASVRLHQEEDSCLPPSC"
     gene            complement(13589..14716)
                     /locus_tag="RHV13_002986"
     CDS             complement(13589..14716)
                     /locus_tag="RHV13_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1242500.1"
                     /translation="MTLCVFVLIASEFMPVSLLTPITRDLGVTEGLAGQGIAISGALA
                     VLTSLTLSTLAGKMNRKFLLLGMTVLMAVSGLIIALATSYLMYMVGRAMIGVAIGGFW
                     SMSAATAIRLVPQHQVTRALAIFNAGNALATVVAAPLGSYLGATVGWRGAFLCLVPMA
                     VVAFIWQCISLPSMDANKSHTSCGAVFRLFSRRMVVVGMIACGLFFMGQFALFTYVRP
                     FLESVTRVNSLGLSLILLIIGVAGFIGTLVVSTFLNRKFYPTLMAIPGLMAVIAIGLM
                     LTGHHVWTVSLLLGLWGMLATAAPTGWWTWIARTLPDNAEAGGGLMVAVIQLSIALGS
                     TAGGIVFDRLGWQSTFAMSSVLLLCAGVLTFVTARQKAGAL"
     gene            complement(14858..15916)
                     /locus_tag="RHV13_002987"
     CDS             complement(14858..15916)
                     /locus_tag="RHV13_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV1242501.1"
                     /translation="MSYADSTNPNAPVSMTDKWDKTFAESQKVDHRKVSFPNRYGITL
                     VGDLYLPKDRGDRKLAAIAVSGPFGAVKEQSSGLYAQTLAERGFVTLAFDPSYTGESG
                     GYPRNVASPDINTEDFSAAVDFLGLQKEVDRNRIGLLGICGWGGMALNDVAMDTRVKA
                     VATSVMYDMSRAMGHGVGDGKDRYSTADRRAVLQYLNEQRWKDAHSGTFAHGGHDINV
                     DSNGKVSAGERILPETLPANPHPVLKEFFDYYRMPRGFHERSVNSTGAWTATMPLSFM
                     NMPLLRYASEITIPTLIVTGEKAHSRYFAEDAYKAVGSKDKELVVVPGANHVDLYDNV
                     AGKIPFAKFEQFFQTKLK"
     gene            16181..17065
                     /locus_tag="RHV13_002988"
     CDS             16181..17065
                     /locus_tag="RHV13_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV1242502.1"
                     /translation="MAKRENYNELYLFMQVVREGSFTAAAQRLGLAQSGVSRSVRELE
                     ERLGVQLLVRTTRKLSLTQAGEQLYQKTASGFEMLDLGLATLAHYRETPSGTVRINAS
                     QHAIDKCLLPKLAVFKQRYPDIRLELINESRFVDIIEERFDAGVRLGPEVGQGMVAVR
                     ITPDMEMAIVGTPEHFRRYGFPQTPADLKAHPCIAYQFADGSVYQWELNQDDKKITHR
                     PEGQWALSDSYMEAEAARLGLGLAYVPVELVADDLEHGNLIRVLQRYSLRMEGLFLYY
                     PHRNVSPALRMVIDTLKI"
BASE COUNT         4284 a         4679 c         4610 g         3744 t
ORIGIN      
        1 tcgttggaga aaattagaaa agatttcgtg tttatcaatc tcaatacgga ttctttatct
       61 gaatttataa accgtgataa tgaatggtta tccgcggtaa aggggaaaca ggtcgtattg
      121 attgcggcca gaaagtcaga agccttagca aattattggt attacaacag caatattagg
      181 ggcgtggtat acgctggact gagtcgtgat attagaaaag aactggccta tgtgattaat
      241 ggcaggttcc tgagaaaaga tattaagaaa gataaaatca ctgaccggga aatggaaatt
      301 atccgcatga cggtccaggg aatgcaacct aaatcgattg ccagaattga aaattgtagt
      361 gtgaagacag tgtataccca tcggcgtaat gctgaggcca agctgtactc aaaaatatat
      421 aagttggttt agtaaactcc aggcaagtta gttttaaaaa atgactcact gggacatcac
      481 gtcctcaatt caactcggga agaaatgcaa tgaaaaaaaa ggttctggca atagctctgg
      541 taacggtgtt taccggcatg ggtgtggcgc aggctgctga cgtaacagct caggctgtag
      601 cgacctggtc ggcaacagcc aaaaaagaca ccaccagtaa gctggttgtg acgccactcg
      661 gtagcctggc gttccagtat gccgaaggca ttaaaggttt taactcacag aaaggtctat
      721 ttgacgtggc tatcgagggt gactcaacgg ctaccgcctt taaactgacc tcacgtctta
      781 tcaccaacac attaacccag ttggatacct caggttccac actgaatgtg ggcgtggatt
      841 ataacggcgc ggcagtcgaa aaaactggcg ataccgtgat gatcgatacc gccaacggcg
      901 tactgggcgg caaccttagc ccgctggcta acggttacaa tgccagcaat cgtaccaccg
      961 cacaggatgg tttcaccttc accatcatca gcggcaccac caatggtacc accgcagtaa
     1021 ccgattacag cactctaccg gaaggcatct ggagcggcga cgttagcgta cagttcgacg
     1081 cgacctggac cagttaatct ctctgatgta ccagcagggg tagcccccct gctttattcc
     1141 tggacggaat tgttatgaaa aagcaccttc tgcctctcgc tctgctgttt tccggaatat
     1201 ctccggccca ggcgctggat gtcggcgata tatcatcgtt tatgaacagt gacagcagca
     1261 cgctgagcaa aacgatcaaa aacagtaccg acagtggtcg ccttatcaat atccgtctcg
     1321 aacggctctc ttcaccgctt gacgacgggc aggttatctc aatggacaag ccggatgagt
     1381 tgctactcac tcccgccagc ttgctgctac ccgcccaagc cagcgaagtg atccgcttct
     1441 tctataaggg acccgcagat gaaaaagagc gctactaccg cattgtctgg tttgatcagg
     1501 ccctcagtga tgcgcagcgc gataatgcca aacgcagcgc tgtggccact gcttccgccc
     1561 gcatcggcac cattctggtc gtcgcccctc gccaggcgaa ctaccacttt cagtacgcca
     1621 acggctccct gacaaataca ggaaatgcga cgctgcggat cctcgcctac ggcccttgcc
     1681 tgaaagccgc caacggtaag gagtgtaaag agaattacta cctgatgccg ggcaagtcgc
     1741 gtcgttttac ccgcgtggac actgcggata acaaaggacg ggttgcactt tggcagggtg
     1801 ataagttcat tcccgtgaaa tagatagctg tgcagatgga taacgacaat gcctttacga
     1861 cggttctccc caggactgaa agcccagttt gccttcggca tggtcttttt gttcgttcag
     1921 cccgatgcca gcgctgctga cataagtgcg cagcaaatag gtggggtgat tattccgcag
     1981 gccttcagtc aggcgcttca ggacggcatg agcgtcccgc tctatattca tctcgccggt
     2041 agccagggtc gccaggacga tcagcgaatc ggcagcgctt ttatctggct ggacgatgga
     2101 cagctacgca tccggaaaat acagctggaa gagagtgaag ataacgccag tgtcagcgaa
     2161 caaactcgac agcagctgat ggctctggcg aacgccccgt tcaatgaggc ccttaccatc
     2221 cccctgactg acaacgcgca gctggatctc agcttgcgcc aactactgct gcagctggtg
     2281 gtcaagcgcg aagcgctggg caccgtacta cgctcacgta gcgaagacat cgggcagtcc
     2341 agtgttaaca ccctcagcag taatctgagc tataacttgg gcgtctataa caaccagttg
     2401 cgtaacggcg ggagcaacac atccagctat ctgtcgctga ataacgttac tgcgctgcgc
     2461 gaacatcatg tggtgctcga cggctcgctg tacgggatcg gtagcggtca acaggacagt
     2521 gaattatata aagcgatgta tgaacgcgat tttgccggtc accgatttgc cggtggaatg
     2581 ctcgacacct ggaacttgca gtccttaggg ccgatgaccg ccatttcagc agggaagatt
     2641 tacggccttt cctggggaaa ccaggccagc tccaccatct tcgacagcag ccagtcagcc
     2701 acgccagtga tcgccttttt accggcggcg ggcgaagtac atctcacccg tgatgggcgg
     2761 ctactaagcg ttcagaactt caccatgggc aatcatgaag tggatacccg gggtctaccg
     2821 tacggtattt acgatgtgga agttgaggtg atcgttaacg gtcgcgtgat cagcaaacgt
     2881 acccagcggg tcaataagct gtttagccgg gggcgcggcg tcggtgcacc actggcgtgg
     2941 caggtatggg gcggtagctt tcatatggat cgctggtcgg aaaacgggaa aaagacgcga
     3001 ccagctaaag agagttggct agcaggtgcc tcgacctccg gctcattgag tacgcttagc
     3061 tgggcggcaa cgggatatgg atacgataat caggcggtgg gtgaaacccg tctgacgctg
     3121 ccgcttgggg gggcaatcaa cgttaacctg caaaatatgc tggccagtga cagctcatgg
     3181 agcagcatcg gcagcatcag cgccactcta ccgggaggct ttagttcgct gtgggttaat
     3241 caggaaaaaa cccgcattgg caatcaattg cgacgtagcg atgccgacaa ccgtgctatc
     3301 ggcggcacac tcaacctgaa ctcactgtgg tcgaagctgg gcacattcag catcagctac
     3361 aatgatgacc gccgttacaa cagccattat tacacggcag attactatca aaatgtctac
     3421 agcggtacct ttggttcgct tggcctgcgg gccggtattc agcgctataa caacggcgac
     3481 agtaacgcca atacagggaa atatatcgct ctcgatctct cgctaccact gggcaactgg
     3541 tttagcgcag ggatgactca tcaaaacggc tacaccatgg caaacctgtc agcacgcaag
     3601 cagtttgatg aagggaccat tcgcactgtt ggtgccaatc tgtcacgtgc catctccggc
     3661 gataccggtg atgacaaaac cctcagcggt ggggcgtatg cacagttcga cgctcgttac
     3721 gccagcggaa cgctgaacgt caatagcgcg gcggacggct acgtcaatac caacttgacc
     3781 gccaatggca gcgtcggctg gcagggtaaa aacattgctg ccagcgggcg gactgatggc
     3841 aacgctgggg tgatattcaa caccgggctg gaggacgacg gtcagatcag cgccaaaatc
     3901 aacgggcgga ttttcccgct taacggcaag cgtaactatc tcccgctctc tccctatgga
     3961 agatatgagg tggagttaca gaacagcaaa aactcactcg acagttacga tatcgtcagc
     4021 ggccgcaaaa gtcgtctgac tctctatcca ggcaatgtcg ctgtcattga gccagaggtg
     4081 aagcagatgg ttaccgtctc cggtcgtatc cgtgcggaag acggcacact gctggctaac
     4141 gcacggatta acaaccatat cggccgaacc cgaaccgatg aaaacggcga gtttgtcatg
     4201 gacgtggata agaaataccc cactatcgat tttcgctaca gtggcaataa aacctgcgaa
     4261 gtggcactgg aactcagcca ggcgcgcggt gccgtctggg tcggtgatgt ggtctgcagc
     4321 ggtctctcat cgtgggcggc ggtgacgcag acaggagaag agaatgagag ttaacctact
     4381 gatagcgatg ataatatttg cgctaatctg gcctgcaacc gcgctcagag ctgcagtgag
     4441 caaaacaacc tgggcggatg caccggcacg cgagtttgtg tttgtcgaaa acaactcaga
     4501 cgacaacttt ttcgtcacgc ctggcggggc gctggatccg cgcctgaccg gtgccaaccg
     4561 ctggaccggt ttaaaataca ctggttcagg aaccatctat cagcaaagcc tcggctacat
     4621 tgataacggt tacaacaccg gcctttatac caattggaag tttgatatgt ggctggaaaa
     4681 ttcaccagtt tcatctcctt taactggctt gcgctgcatc aactggtacg ctgggtgtaa
     4741 tatgaccacc agtcttatcc tgccgcaaac caccgacgcc agtggatttt atggcgcgac
     4801 ggtgaccagc ggcggcgcga agtggatgca cggcatgttg tcagacgcgt tttaccagta
     4861 tctgcaacaa atgcccgtcg gcagcagctt tacaatgacc atcaatgcct gccagacctc
     4921 tgtgaactat gacgccagca gcggcgcacg ctgtaaggat caggcctccg gcaactggta
     4981 tgttcgcaac gtcacccata cgaaagcagc aaatctacgg ttgataaata cccactcgct
     5041 ggcggaagta tttatcaaca gcgacggagt accgactctg ggcgaaggga acgccgactg
     5101 ccggacgcaa accatcggca gccgttcagg attaagttgt aagatggtta actataccct
     5161 gcaaacaaac ggactcagca acacctcaat ccatatattc ccggcgatcg ccaactcgtc
     5221 gttagcctcg gccgtcgggg cgtacgatat gcagttcagt ctgaatggca gttcatggaa
     5281 accggtgagc aataccgcct attactacac cttcaacgag atgaagagcg cagactcgat
     5341 ctatgttttc ttctcgagca acttctttaa gcagatggtg aacctcggga tcagcgatat
     5401 caacaccaaa gatctattca actttcgctt tcagaacacc acatcaccgg agtctggctg
     5461 gtatgaattt tctacctcca acacgctgat tatcaaaccc cgtgatttca gcatcagtat
     5521 tatctccgat gaatatactc agacaccgtc gcgggaggga tatgttggca gcggcgagtc
     5581 ggcactcgat ttcggctata tcgtaaccac cagcggtaaa acagctgccg acgaagtgct
     5641 gatcaaggtg accggacccg cgcaggtgat tggcgggcgc tcctattgtg tcttcagctc
     5701 cgatgacggt aaggcgaaag taccgttccc ggcgacactt tcctttatta cccgcaacgg
     5761 agctacaaaa acctacgatg ccgggtgcga tgatagctgg cgggatatga ccgatgcgct
     5821 gtggttgacc acaccgtgga ctgatatctc tggcgaagtg gggcagatgg ataagaccac
     5881 agtcaaattt tcgattccaa tggataacgc catttcgctg cgtacggtag atgataacgg
     5941 ctggtttggc gaagtcagcg cttcaggaga aattcatgtt caggcgacgt ggcgtaacat
     6001 taactaaggc cctgctgaca gcggtctgta tgctggcggc acctttgaca caggcgattt
     6061 cggtcggcaa tctgacattt tcgctgccgt ccgagactga ctttgtcagc aaacgtgtag
     6121 tgaataacaa caaaagcgca cggatatacc gtattgccat cagtgctatt gatagcccgg
     6181 gcagcagtga attgcgcacc cgaccggtgg atggtgaact gcttttcgcc ccccgccagc
     6241 tggcgttgca ggctggtgag agcgagtatt ttaaatttta ctatcatggt ccacaggata
     6301 accgcgagcg ctactaccgg gtctcatttc gcgaggtccc cactcgtaac cagacaagac
     6361 gcagcccaac cggcggcgag gtcagcacgg agccggtggt ggtgatggat accattctgg
     6421 tagtacgacc acgtcaggtt cagtttaaat ggtccttcga tcaggtgacc ggaacggtaa
     6481 gtaacaccgg caacacgtgg tttaagctac tgattaaacc aggatgtgat tcgaccgaag
     6541 aggaaggcga tgcctggtat ctacgtccgg gagacgtggt tcatcagcct gagttacgtc
     6601 agccggggaa tcattatctg gtctataacg acaaattcat taagattagc gattcctgtc
     6661 cggctaagcc cccttcggcg gactaagact tatccatcgg tcgagggaag aattccactt
     6721 cagagcccca aaaatcgttt aaatgatgtg gaagcgatcg tcaatatcac ctgtaacgat
     6781 gggctttctc atatgcacac catagtgttc aggagacacg gaaaggtatc aggcagcctg
     6841 ctaacgagtg gtttattcac gaattagtac gtaaaatcgg taacggctgg aaatcattca
     6901 atactcacac tatcgaaagt tccccagcca accgcggtac gttcttacat acgatgtacc
     6961 gctgttctct ttacgattta tagctgtact ggtgaattat gagcaatctg aatccatgca
     7021 tgacgtgtgg tgcctgttgt gcatttttcc gcgtctcttt ttactgggcc gaagccgacg
     7081 atgctggtgg taccattccc gccaggctca ctgaacaaat atcccctttt caccgatgca
     7141 tgagcggaac taatcagaaa aacccccgat gtattgccct tgcaggaacc ccgggcaaaa
     7201 atgcctgctg cacgatatat aaaaatcgat cgtccacatg cagagaattc gccatgtctg
     7261 gtgagaacgg agtcgtcaat gaggcttgca atcgtgcaag ggctaaatac gggctgacac
     7321 cgttataaac atacaataat taattgcact gccccgccta ctgtaatagc ggggcttcgt
     7381 tttcagggac aacaaaaccc gaaacttatt gtatttgcct gcaacaatgc ctctcttctg
     7441 tagcgttctt cggatctaca tcatcctgaa gtagcacgat atactcactg actatctgaa
     7501 tcagaatata aaaagcaatg tttttaacct ataaaaatgg cgctgtattt gcgccatttt
     7561 tatcattcaa tgcattatct gtttgagcct aaagggatct cagggtctgg ctcatgagta
     7621 attctgtttc gtaaatctct gcgaataatt tcaatggacc agaaccagac taaatgtcca
     7681 aaaatttcag aaacattctc ataccacggg agatcaaaca gaggtggtgt cagtcccatg
     7741 agagggaatg aaatcatatg aacaaaaagt tgggctaaag cacctgccag taagccctgc
     7801 cagagtttaa tttttggaaa tacttcagcg accacacaat aaccgacagc aaacactatc
     7861 gaaaagataa tgtgcgtaac accaacccag ttaaagacat gcccggcaaa ggtataaaca
     7921 gccgcattgg gatctgtcag ccccagccag tctcgaagaa aaatatacgg aggattgaga
     7981 aaattacgcg agcaatcaat ttgcccagca gccctgatta atgattccgg gccacacgct
     8041 gcattaaaca tatccaccgg gctacgtggc ggcaatggaa cttcagcccc ccacttcaca
     8101 aatgcggaaa caacgccagc aatcagccca atgaatgcag caagaccata acgtctgcgg
     8161 ttcggtggag tttgttcaaa tatattcata tctaccctgc ttgtaccatt atgttataca
     8221 cctcttcagg agtattcata aaacaaggaa aatgtaaaga actgtattgt tttgtataac
     8281 aagatggttg tctaatcacc aaagaatata agcaccatca tttctcccta tttttatatt
     8341 agaagtgaca tagatttgca gggtgatgca gagctgacaa tcacaggttt ccttatcggt
     8401 ttttgcatcg tacaactaaa gcaataaaac agctcccatc atcatcaaca tcccggcgaa
     8461 catgaattta ttagccgggc tcatagctgc attcgcgttt taagaataat cctcctgctg
     8521 tcgccgacta tgcttaacgt ttaaaaaagc atcagcactc tcgcaacgca ctcttatttt
     8581 cccctttaga ataccggagg cctggtatga gcaaccaagg cgaatacccc gaagataatc
     8641 gggttgggaa gcacgagccg cacgatttta gtttgacccg tcgcgatctg attaaagtga
     8701 gcgccgcaac ggcggcgacc gccgtggttt atcctcattc tacgcttgcg gcaagcgttc
     8761 cggcagctac acccgcgcct gagataatgc ccctgacact taaggtgaac ggcaaaaccg
     8821 agcagcttga agtggatacc cgaaccacgc tactggacgc tttgcgtgaa aatctgcatt
     8881 tgatcggtac caagaaaggt tgcgatcacg gacagtgcgg agcctgtacc gtgctggtca
     8941 atggtcgcag gcttaatgcc tgcctgacgc ttgcagtcat gcatcagggg gccgagatca
     9001 ccaccattga aggcctgggt tcaccagata atcttcaccc catgcaggcg gcctttatca
     9061 agcatgatgg cttccagtgc ggctactgta cctccgggca aatttgctca tcagtagcgg
     9121 tgctaaaaga gattcaggac ggcattccca gtcacgtcac ggtcgatttg gtttcccctc
     9181 cagaaacaac tgccgatgag atccgtgaac gtatgagcgg caacatctgt cgctgtggtg
     9241 catacgctaa catccttgcc gccattgaag acgctgcggg ggagataaaa tcatgaaggc
     9301 gtttacctat gaacgagtga acaccccggc cgaggcggca cttagcgctc agcgcgtacc
     9361 cggcgcaaaa tttatcgcgg gcgggaccaa tctgctggac ctgatgaagc tggaaattga
     9421 aacgcccacc caccttatcg atgtgaacgg gctcgggctc gataagattg aagtgaccga
     9481 cgcgggtggg ctgcgcatcg gcgcactggt acggaacacc gacctggcgg ctcacgagcg
     9541 cgtgcgtcgt gattacgcgg tactctcccg cgccctgctc gctggcgcgt ctggtcagtt
     9601 acgcaatcag gcaaccaccg caggtaatct gctccagcgc acgcgctgcc cctattttta
     9661 cgacaccaat cagccctgca ataagcgcct gcccgggagc ggctgcgcgg cgcttgaagg
     9721 ctttagccgt cagcacgcgg tggtaggcgt aagcgaagcc tgcattgcca cccatccgag
     9781 cgatatggcg gtcgcaatgc ggttgctgga tgcggtggtg gaaaccatca cgccggaggg
     9841 aaagactcgc agtatcacac tggctgattt ttatcaccca ccggggaaaa cgccgcacat
     9901 tgaaaccgcc ctgcttcccg gtgagcttat cgttgcggtg acgttacctc cgccgctcgg
     9961 cggaaaacat atttaccgta aggtgcgcga tcgcgcctcc tacgcctttg ccctggtatc
    10021 ggtcgcggcg attattcagc ctgacggcag cgggcgcgtc gcgctgggcg gagtagcaca
    10081 taagccctgg cgcattgagg ctgcggatgc tcagctatcc cagggggcac aggccgtata
    10141 tgacacgctg ttcgccagcg cccatcccac cgctgaaaac acctttaaac tcctgttggc
    10201 gaagcgaacg cttgcctccg tactggctga agcgagggca caggcatgaa atttgataaa
    10261 cccgcagggg aaaacccgat cgatcagctg aaggttgtcg gtcgtcccca tgaccgcatc
    10321 gacggaccgc tgaaaactac cggcacggca cgctacgcct acgaatggca tgaagaggcc
    10381 cccaacgccg cctatggcta tatcgtcggt tccgccattg ccaaaggacg cctcaccgcc
    10441 cttgatacgg acgccgcgca aaaagcgccg ggcgtactgg ctgtcattac cgccagtaac
    10501 gccggggcac tcggcaaagg cgacaaaaac accgccaggc tgttaggcgg ccccactatt
    10561 gagcactatc atcaggccat tgcgctggta gtggccgaga ccttcgaaca ggcgcgagcg
    10621 gcggcctcgc tggtgcaggc acactatcgc cgtaataaag gagcttactc cctggcggac
    10681 gaaaaacagg ccgtcaatca gccgccggaa gacacgcccg acaaaaacgt cggtgacttt
    10741 gacggggctt tcacctccgc tgcggtgaag attgatgcta cctacacgac cccggaccag
    10801 agccatatgg cgatggagcc gcatgcctcg atggccgtct gggatggaaa taagcttact
    10861 ctctggacct caaatcagat gattgactgg tgccgcaccg atctggcaaa aacgctaaaa
    10921 gtgcccgtgg agaatgtgcg tattatctcc ccgtatatcg gcggagggtt tggcggcaag
    10981 ctgttcctga gaagcgatgc gctgctggca gccctcgccg cccgagcggt gaaacgtccg
    11041 gttaaagtga tgctcccccg ccccactatt cccaataaca ccacgcaccg ccccgccacc
    11101 cttcagcact tgcgtatcgg tgccgaccag agcgggaaaa tcaccgctat ctcacatgaa
    11161 agctggtccg gaaacctgcc cggcggcacg ccggaaacgg cggtacagca aagcgaatta
    11221 ctctacgccg gggcaaaccg tcataccggc ctgcggctcg ccacgcttga tttgccggaa
    11281 gggaacgcca tgcgtgcgcc cggcgaagcc cccggtctga tggcgctcga aatcgcgatc
    11341 gacgaactgg cggaaaaagc gggcatcgat cccgtcgagt ttcgcatcct gaatgacact
    11401 caggttgacc ccgccgaccc gacgcgccgc ttctctcgcc gtcagcttat tgagtgcttg
    11461 cgcaccggag cggataaatt tggctggaag cagcgcaacg ccacacccgg acaggtacgc
    11521 gacggagagt ggctagtcgg ccacggcgtc gcggcgggct ttcgcaataa tctgctggaa
    11581 aaatcggggg ctcgggttca cctcgaacca aacggcaccg ttaccgtgga aacggacatg
    11641 accgacattg gcaccggcag ctacaccatt ctggcccaga cggcagcgga aatgcttggc
    11701 gtaccgctgg agcaggttgc ggttcacctc ggcgattcca gtttcccggt ttctgcgggt
    11761 tctggtggac aatggggcgc gaatacctcc acctccggcg tttacgccgc ctgtgtgaag
    11821 cttcgcgaaa tgattgcctc ggcagtcggg tttgatcctg agcagtcgca gtttgccgac
    11881 ggcaagatta ccaacggtac ccgaagcgcc atgctacatg aggccaccgc aggcggcaga
    11941 ctgacagcgg aagagagcat tgaattcgga acactgagca aggagtacca gcagtcgacc
    12001 tttgccgggc attttgtgga ggtcggcgtg catagcgcga cgggagaagt tcgggtccgg
    12061 cgtatgctcg ctgtgtgtgc tgcaggacgc atcctgaatc cgaaaactgc acgcagccag
    12121 gtcattggcg caatgactat gggcatgggc gcggcactga tggaggagct ggcggtggat
    12181 gaccgtttgg gctacttcgt taatcacgat atggtggggt atgaggtgcc ggtccatgcg
    12241 gatatcccaa aacaggaggt gattttcctg gatgataccg accccatatc ctccccgatg
    12301 aaggccaaag gtgtcggtga gctgggcctg tgcggcgtga gcgctgctat cgccaacgcg
    12361 gtgtataacg ccaccggtat tcgggtacgc gattatccca tcactctgga taagctgctc
    12421 gataagctgc cggatgtggt ttaaggagga acaatgtcat acccgctttt tgataaagac
    12481 gaacactggc ataagccaga gcaggcgttt ctcaccgatg accaccgtac cattctgcgc
    12541 ttcgccgtag aggcgctaat gtccggtaaa ggagcggtgc tggtgacgct ggtggagata
    12601 cgcggcggtg cggcgcgccc gctcggggcg cagatggtgg tgcgcgaaga tggtcgttac
    12661 tgcggttttg tctctggcgg ctgcgtggag gccgctgccg cttttgaagc gctggagatg
    12721 atgggctcag gccgcgatcg cgaaattcgc tatggcgaag gttcgccgtg gtttgacatc
    12781 gttctgccct gcggcggtgg gatcacgctg acgctccata aactacgctc ggcacagcct
    12841 ctgctcgccg tgctgaaccg cctggaacag agaaaaccgg cggggctgcg ctacgatccg
    12901 caagcacaat cgttggtgtg cctgcccacg caaacccgaa cgggctggaa tctcaatggc
    12961 tttgaggtgg ggttcaggcc atgcgtcagg ctgatgattt acggacgttc tcttgaggcg
    13021 caggcaaccg cgagtcttgc agcagccaca ggctatgaca gccatatctt cgatcttttt
    13081 ccggcctcag ccagcgctca gatcgatacc gacacggcgg tcattttgct gtgccatgat
    13141 ctcaaccggg agctgccagt gttgcaggcc gcgcgagaag caaaaccctt ttatctcggc
    13201 gcattgggca gctatcgaac ccacacgtta cgtctgcaaa agctccacga gcggggatgg
    13261 tccagggagg agacagcgca aatccgggca cccgtcggga tattccccaa agcccgggat
    13321 gcgcatactc tggcactctc cgtgctggca gaagtcgcct ctgtacgtct ccatcaggag
    13381 gaggattcat gcctgccccc gtcgtgctga tccttgcggc cgggcgtgga aagcgctttc
    13441 tcacctccgg gggaaatacc ataaattaag agaaaaatct gcagtagccg ttttagaaaa
    13501 tcacctaaat catttaaaag agcgacctac ccatagtggg tgctcatcgc cttgtgtaac
    13561 ttattcagca acgcgcgccc tcgccggact aaagcgcacc ggccttctgg cgtgcggtaa
    13621 caaaggtcag tacgcccgcg caaaggagca gcacactact catggcaaac gtactctgcc
    13681 agccaaggcg gtcaaacacg atgcctcctg ccgttgatcc aagggcgatt gagagctgaa
    13741 tcaccgcgac catgagcccg cctccggctt cggcgttgtc aggcaacgta cgcgcaatcc
    13801 atgtccacca tccggttggc gcggcggtgg ccagcatgcc ccagagtcct aacagcagcg
    13861 agaccgtcca gacgtgatgc ccggtaagca tcagaccgat ggcaatgacg gccatcagtc
    13921 cgggtatggc catcagcgtg gggtagaatt ttctgttgag aaatgtcgaa acaaccagcg
    13981 tgccaataaa accggccacg ccgataatca gcaaaatcaa cgacaagcct aaggaattaa
    14041 cccgtgtcac cgactccaga aacgggcgca cataggtgaa tagcgcaaac tgccccatga
    14101 aaaacaggcc acaagctatc atgccgacca caaccatgcg gcggctaaat aagcggaata
    14161 ccgcgccaca tgacgtgtgg cttttattcg catccatgct gggcagacta atgcactgcc
    14221 agataaaggc gacaaccgcc atgggcacca ggcacaagaa agcgcctcgc catccgaccg
    14281 tagcccccag atagcttccc agcggcgcgg ccacgaccgt cgccagcgca ttaccggcgt
    14341 tgaaaatcgc cagcgcacgc gtgacctgat gctgcggcac cagacgaatc gccgttgccg
    14401 ctgacatcga ccagaatccg ccaatggcca caccaatcat cgcacgaccg accatataca
    14461 tcagataact ggtagccagc gcaataataa gccccgatac ggccatcaga accgtcatcc
    14521 ccagcagcag gaacttacga ttcatctttc cagctagcgt tgaaagcgtc aggctggtca
    14581 gaacagccag cgcgccggag atagcaatcc cctgccctgc aagcccctcc gtcacgccta
    14641 aatcgcgggt aatcggcgtg agcaggctga cgggcataaa ttcagaagca atcagcacaa
    14701 acacgcacag ggtcatagca aaaataccgc cccagtatgc gtgctgatga tgaggtgatg
    14761 gtgtttggct tagtgtcgac atagtgaaat ttcacaaaga tcaggataaa agccacctgc
    14821 catagaccgg tggctttgca tcaattcagg agaggtttta tttcagtttg gtctggaaaa
    14881 attgttcgaa cttcgcgaaa ggtatttttc cggccacgtt atcgtacagg tccacgtgat
    14941 ttgcccctgg taccaccacc aactctttgt ctttactgcc aaccgccttg taagcatctt
    15001 cagcaaaata gcgtgaatgc gctttctcac cggtcacaat cagcgtcggg atggtgattt
    15061 cactggcgta tctcaacagc ggcatattca taaatgacag cggcatcgtt gccgtccacg
    15121 ccccggtaga gttaacggaa cgttcgtgga agccgcgcgg catgcgatag tagtcaaaga
    15181 actctttcag taccggatga ggatttgccg gcagcgtttc tggcagaata cgctcgcccg
    15241 cactgacctt gccgttgcta tcgacattaa tatcatgacc accgtgagcg aacgttccgc
    15301 tatgcgcatc cttccagcgc tgttcattca gatattgcag aacagcacga cggtcggcgg
    15361 tagaataacg gtctttgcca tcccccacac catgacccat cgcccggctc atgtcataca
    15421 tcacgctggt agctaccgct ttaacgcggg tatccatcgc cacgtcgttc agcgccatgc
    15481 cgccccagcc acaaataccg agcagcccga tacggttgcg gtctacctct ttttgcagcc
    15541 ctaagaaatc caccgccgcg ctgaaatctt cagtgttgat atccggtgag gcgacgtttc
    15601 gcggatagcc gccgctttcc ccggtgtaag aaggatcgaa cgccagggta acaaaccctc
    15661 gttccgccag cgtctgcgca tacaggccgc tggattgctc tttcaccgcg ccaaatggcc
    15721 cactgaccgc aatcgccgcc agcttgcgat cgccacggtc tttaggcagg taaagatcgc
    15781 ccactaaggt gatgccatat cggttcggga acgagacttt acggtgatcg actttctggc
    15841 tctcggcgaa cgttttatcc catttatctg tcatggacac aggggcatta ggattggttg
    15901 aatcagcata actcattgtc gtgactccac ttaatgatgc gaaaagcagc atggtcggca
    15961 tcgcgttttt tagcgttcgg gtgaaggctt tcataaaaat ccccataaaa aacaacagag
    16021 agatacaggt ttccgacgga caacgctgcg gtgctttact ttgatggggt cattatagtg
    16081 agcagaatta gtgctgatta gggggcgatt aatgctaaga ttaataccat attgttataa
    16141 attaatgcgg agattgccgg catgaagggg ggatgtttca atggcgaaaa gggagaacta
    16201 caacgagctg tacctgttta tgcaggtggt gcgggaaggc agctttaccg cagcggctca
    16261 gcggctgggt cttgctcagt caggagtcag ccgttctgtt cgcgagcttg aagaaaggct
    16321 gggtgtccag ctgctagtgc gcaccacgcg taaactgtcg ctgacacaag caggcgagca
    16381 gctctaccag aagacggcat ctggatttga aatgttagat cttgggcttg ccacactggc
    16441 gcactatcga gagacgccct ccggcacggt gcgtatcaat gccagccagc acgcgattga
    16501 taaatgcctg ctgccaaagc tggcggtatt taaacagcga tatcctgata tccggcttga
    16561 actgataaat gagagccggt tcgtcgatat catcgaggaa agattcgatg caggtgtccg
    16621 cctggggcct gaagtcggcc agggtatggt cgcggtacgc atcacgcccg atatggagat
    16681 ggcaattgtg gggactccgg aacattttcg tcgttatggt tttccgcaaa ccccggcgga
    16741 tttaaaggcg catccctgta tcgcctatca gtttgccgac ggcagcgtat accagtggga
    16801 actcaatcag gatgataaaa aaatcactca tcgccctgaa gggcaatggg ccctatccga
    16861 cagctatatg gaagcagaag ccgcccggct gggcctcgga ttggcctatg ttcctgttga
    16921 actggtcgcg gatgatttag aacacggtaa ccttatcagg gtgttgcagc gttacagcct
    16981 gcgaatggag ggattgtttc tctattaccc tcatcgcaac gtctcccccg ctctgcggat
    17041 ggtcattgat acattgaaaa tctgaacgtt aacaacgacc gactcttcac tgtgcaatat
    17101 tgtgtcaggt agtcactctg cgttcaattc caggggccgc tttgagcgag aaagagcgga
    17161 cttatctgta taaggggact atccagcaat ggagatcacc caaaagaaaa aactacctgg
    17221 ctccctcaac aaccttcatc cgggaattta gcctcaatca acatttttat atctgtcagg
    17281 ttacttcatt atgaaaaccg atcaatatcg ttgtttt
//
LOCUS       DAPGOF010000103        16970 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.104, whole genome shotgun sequence.
ACCESSION   DAPGOF010000103 DAPGOF010000000
VERSION     DAPGOF010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16970
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.104"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..725
                     /gene="espX2"
                     /locus_tag="RHV13_002989"
     CDS             <1..725
                     /gene="espX2"
                     /locus_tag="RHV13_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspX2"
                     /protein_id="HDV1242503.1"
                     /translation="MLEPEVKKKTIWNNYSIYPSLQDTHEVVRDDPETICMRAFPLFA
                     KGWEYAQKNKKHQLILNALGFKGYIRDIFMSAIMRKTDFVLECNNQPTELNSTFSSLM
                     NDSDQWQQHTLKVKHYANLLTMLDLNEASESDKSKIFFCLSAIFANISHSNVFNGIPD
                     ASKTLKRYAFALLAKAYSLDESMISSQTFNTYKTVLLDFNNLSNEEANQLRISSLYRD
                     MVRYAQYRFSKVLSEWTPDAWL"
     gene            1009..3159
                     /gene="dinG"
                     /locus_tag="RHV13_002990"
     CDS             1009..3159
                     /gene="dinG"
                     /locus_tag="RHV13_002990"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HDV1242504.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQVYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLTQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            3187..4149
                     /gene="ybiB"
                     /locus_tag="RHV13_002991"
     CDS             3187..4149
                     /gene="ybiB"
                     /locus_tag="RHV13_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HDV1242505.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATTSDGLARVNQAF"
     gene            4290..5375
                     /gene="hcxB"
                     /locus_tag="RHV13_002992"
     CDS             4290..5375
                     /gene="hcxB"
                     /locus_tag="RHV13_002992"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HDV1242506.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEANTRRERQKQGIPLDGGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(5604..5864)
                     /locus_tag="RHV13_002993"
     CDS             complement(5604..5864)
                     /locus_tag="RHV13_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDV1242507.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(6129..6395)
                     /gene="ybiI"
                     /locus_tag="RHV13_002994"
     CDS             complement(6129..6395)
                     /gene="ybiI"
                     /locus_tag="RHV13_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HDV1242508.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(6469..7146)
                     /gene="ybiX"
                     /locus_tag="RHV13_002995"
     CDS             complement(6469..7146)
                     /gene="ybiX"
                     /locus_tag="RHV13_002995"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HDV1242509.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(7188..9470)
                     /locus_tag="RHV13_002996"
     CDS             complement(7188..9470)
                     /locus_tag="RHV13_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HDV1242510.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSVAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTATLNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATINNGKDVAQDGSSSLPYTPEHAFTLWSQYQATEDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            complement(9735..9995)
                     /locus_tag="RHV13_002997"
     CDS             complement(9735..9995)
                     /locus_tag="RHV13_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDV1242511.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            10272..11198
                     /gene="rlmF"
                     /locus_tag="RHV13_002998"
     CDS             10272..11198
                     /gene="rlmF"
                     /locus_tag="RHV13_002998"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HDV1242512.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDAILCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVTFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFLNDEQRRRFVNRQR"
     gene            complement(11195..13420)
                     /gene="ybiO"
                     /locus_tag="RHV13_002999"
     CDS             complement(11195..13420)
                     /gene="ybiO"
                     /locus_tag="RHV13_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HDV1242513.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELINQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEF
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLVIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPTEPLPPGEPT
                     L"
     gene            complement(13681..14403)
                     /gene="glnQ"
                     /locus_tag="RHV13_003000"
     CDS             complement(13681..14403)
                     /gene="glnQ"
                     /locus_tag="RHV13_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HDV1242514.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            complement(14400..15059)
                     /gene="glnP"
                     /locus_tag="RHV13_003001"
     CDS             complement(14400..15059)
                     /gene="glnP"
                     /locus_tag="RHV13_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HDV1242515.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            complement(15198..15944)
                     /gene="glnH"
                     /locus_tag="RHV13_003002"
     CDS             complement(15198..15944)
                     /gene="glnH"
                     /locus_tag="RHV13_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HDV1242516.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            complement(16348..16851)
                     /gene="dps"
                     /locus_tag="RHV13_003003"
                     /gene_synonym="pexB"
     CDS             complement(16348..16851)
                     /gene="dps"
                     /locus_tag="RHV13_003003"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HDV1242517.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
BASE COUNT         4114 a         4223 c         4505 g         4128 t
ORIGIN      
        1 caatgcttga gccagaggtt aagaaaaaga ctatctggaa taattactct atttatccat
       61 ctttgcagga tacccatgaa gttgtcagag atgatccaga aacgatttgt atgcgtgcat
      121 ttcctttatt tgcaaaagga tgggaatatg cgcagaaaaa taagaaacat caattgatcc
      181 tcaatgctct tggattcaaa ggttatattc gtgatatatt tatgtcagca ataatgcgaa
      241 aaacagattt tgtgctcgaa tgcaataatc aaccgacaga acttaattca acattttcct
      301 ctttgatgaa cgattcagat caatggcaac agcatactct gaaagttaaa cattacgcta
      361 atctattaac gatgctggat ctcaacgagg ccagtgaaag tgataagtct aaaatatttt
      421 tctgtctgtc tgccatattt gcaaatattt ctcacagtaa tgtctttaac ggtattcctg
      481 atgcgtcaaa aactttaaaa cgttatgcat ttgctttatt ggcgaaagct tattctttag
      541 atgaaagtat gataagcagt caaactttta atacatacaa aacggtattg cttgatttca
      601 ataatctgtc taatgaagag gccaaccagt tgcgtataag ttcgttatat cgtgatatgg
      661 tccgctacgc acaatatcgt ttttctaaag ttttatccga gtggacgcca gacgcatggc
      721 tgtaaagatc gcagctgaat aatggcattg atggccctgg ctcaggccag ggcgggcaat
      781 accggtttct gcgaagcgat gacggtggca agtgtaacca cgaaatgcgg tatcacttgc
      841 tgttttctgc cctgaagact ggcgctcact gtgccgtcga actttcaatc aaatatattg
      901 cgagagggcg tgtttgcgag ccgctttcca gaaacagaaa aaccattacc cctgaaaacc
      961 gaaaaatgcc acaatattgg ctgtttatac agtatttcag gttttctcat ggcattaacc
     1021 gccgcgctta aagcgcaaat tgccgcctgg tataaggcgc ttcaggaaca gatccccgac
     1081 tttattcccc gtgcgccgca gcggcagatg attgcggacg tcgccaaaac gctggccgga
     1141 gaagaagggc ggcatctggc gattgaagcc cccaccggcg ttgggaaaac gctctcttat
     1201 ttaatccccg gcatcgccat tgcccgcgaa gagcaaaaaa cgctggtggt gagtaccgcc
     1261 aacgtggcgt tgcaggatca ggtctacagc aaagatttgc cgctgctgaa aaagatcatt
     1321 cccgatctta aattcaccgc cgctttcggg cgtgggcgct acgtttgtcc gcgtaatctg
     1381 acggcgctcg ccagcactga acccacgcaa caggatctgc tggcgtttct tgacgacgaa
     1441 ctgacgccga acaatcagga agagcaaaaa cgttgtgcga agctgaaggg cgatctcgac
     1501 acttataaat gggatggtct gcgcgatcac actgatatcg ctatagatga cgatctctgg
     1561 cgtcgtttga gtaccgacaa agccagttgc ctcaaccgca actgttacta ctatcgcgaa
     1621 tgcccgtttt ttgttgctcg tcgggagatt caggaagcag aagtggtggt ggcaaaccat
     1681 gcgctggtga tggcggcgat ggaaagcgaa gccgtattgc cagacccgaa aaatttactg
     1741 ctggtgctgg atgaaggtca tcacctgccg gatgtggcgc gggatgcgct ggagatgagc
     1801 gccgaaatca ccgcgccctg gtatcgattg caactggact tgttcacgaa actggtcgct
     1861 acctgcatgg agcagtttcg cccgaagacc atcccgccgc tggcgatccc tgaacgtttg
     1921 aatgcgcatt gtgaagagct gtatgagctt atcgcctcat taaacaacat tctcaatctc
     1981 tacatgcctg ccgggcagga ggcagaacac cgttttgcga tgggcgaact gcctgatgaa
     2041 gtgctggaga tctgccagcg gctggcaaaa ctcaccgaga tgctgcgtgg tctggcggag
     2101 ttatttctta acgatttaag tgagaaaacc ggcagccatg acattgtacg tctgcatcgg
     2161 ttgattttgc agatgaaccg cgcgttgggg atgttcgagg cgcaaagcaa actctggcgg
     2221 ctggcttcgc tggcgcaatc ttccggtgca ccggtgacca aatgggcgac gcgggaagag
     2281 cgcgaagggc agctacacct ctggtttcac tgcgtgggaa tacgtgtcag cgatcagctg
     2341 gaaaggctgc tgtggcgcag tattccgcac attattgtca cctccgcaac cttgcgttcg
     2401 ctgaacagtt tttcgcgttt gcaggagatg agcgggctga aagagaaagc gggcgaccgc
     2461 tttgtggcgc tggattcgcc ctttaaccac tgcgaacagg gcaaaattgt tattccccgg
     2521 atgcgcgttg agccttccat cgacaacgaa gaacagcata ttgctgaaat ggcggcgttc
     2581 tttcgtgagc aggtggagag taaaaaacat ctcggtatgt tagtgctgtt tgccagcgga
     2641 cgggcgatgc agcgctttct cgactatgtg acggatttac gtctgatgtt gctggtgcag
     2701 ggcgatcagc cgcgttaccg tttagttgaa ctgcaccgca aacgcgtcgc caacggtgag
     2761 cgtagtgtgc tggtgggctt acagtcattt gccgaagggc ttgatttgaa aggtgatctg
     2821 ctcacccagg tgcatatcca caaaatcgct tttccgccca tcgacagccc ggtggtgatc
     2881 accgaagggg aatggctgaa aagcctcaac cgctatccgt ttgaggtgca aagcctgccg
     2941 agcgcctcgt ttaacctgat tcagcaggtt gggcgactga ttcgaagcca cggttgctgg
     3001 ggcgaagtgg tgatttacga taaacgcttg ctcaccaaaa actacggcaa gcggctactg
     3061 gatgcattac cggtatttcc gatagagcaa ccggaagtcc ctgaaggtat agttaaaaag
     3121 aaagaaaaaa cgaaatcccc acgccgtcgg cggcgttaat gatgtgagtc aggtaaggag
     3181 tcgtaaatgg actatcgcaa aatcattaaa gagatcgggc gcgggaaaaa ccacgcgcgc
     3241 gatttagacc gggatactgc gcgcggtctg tatgcccata tgctcaatgg tgaagtccct
     3301 gaccttgagt tgggcggcgt attgattgcg ctgcgtatca aaggggaagg ggaagcagag
     3361 atgctcggct tttacgaagc catgcaaaat cacaccatca agctgacgcc gcctgcaggc
     3421 aagccgatgc cgattgtcat ccccagttac aacggcgcgc gtaaacaggc caacctgacg
     3481 ccgttgttgg cgattctcct gcacaaactc ggttttcctg tggtggttca cggggttagc
     3541 gaagatccaa ctcgcgtgct gaccgaaacc attttcgaac tgatgggtat tacgccaacg
     3601 ttgcacggcg ggcaggcgca ggcgaagctc gacgaacatc aaccggtgtt tatgcctgtc
     3661 ggcgcgtttt gcccgccgct ggaaaaacaa ctggcgatgc gctggcggat gggcgtgcgt
     3721 aacagtgcac ataccctggc gaaactggcg acgccatttg ccgaaggtga ggcactgcgt
     3781 ctttccagcg tttcgcatcc ggaatacatt gggcgagtcg ctaagttctt tagcgatatc
     3841 ggcgggcggg cgttactaat gcatggtact gaaggcgaag tgtatgctaa tccgcagcgc
     3901 tgcccgcaga tcaatctcat tgaccgtgaa gggatgcggg tgctgtatga aaaacaggac
     3961 cctgctggta gcgagttatt gccacaagca aaagatccgg aaaccacggc gcagtggatt
     4021 gagcgttgcc tggctggcag cgaaccgatt cccgaatcgc tgaaaatcca gatggcttgc
     4081 tgcctggtgg ctacgggtga agcggcaact accagcgatg gcctggcgcg cgttaatcag
     4141 gcattttaat ttttatctca gccggatgag ccacgtcatc cggcttttac cccaccgcaa
     4201 tatgaaattc ctgcatcttt attgaccttc ccgcgcccgg cgtgcagcat aaaaaaacaa
     4261 caaacacata acataaacag gagttaacca tggaaagtgg tcatcgcttt gatgctcaga
     4321 cgctgcacag ctttattcag gctgtatttc gtcagatggg tagcgaggaa caagaagcga
     4381 aattagttgc cgatcattta atcgcggcaa acctggcagg gcatgattca catggtattg
     4441 gcatgatccc aagctatgtg cgctcctgga gtcaggggca tctgcaaatt aaccatcatg
     4501 ccaaaatcgt taaagaggcg ggggcggcgg tcacgctcga tggcgatcgc gcatttggtc
     4561 aggtcgcggc acacgaagcg atggcgctgg ggattgagaa agcgcatcag cacggtattg
     4621 ctgccgtggc gctacataac tcgcatcata tcggccgtat cggttactgg gcggagcagt
     4681 gtgcagcggc ggggtttgtc tctatccact ttgttagcgt ggtcggtatt ccaatggtcg
     4741 cgccgttcca cggtcgcgac agccgctttg gcaccaatcc gttctgtgtg gttttccctc
     4801 gtaaagataa tttcccgctg ttgcttgatt acgccaccag cgccatcgca tttggtaaaa
     4861 ctcgcgtcgc ctggcataaa ggcgtccccg tgccgccagg ttgcctgatt gacgttaacg
     4921 gcgtgccgac gaccaatccg gcggtaatgc aggagtcgcc gttggggtcg ctgttgacct
     4981 ttgccgaaca taaaggctac gcccttgcag cgatgtgtga aattcttggc ggggcgcttt
     5041 ctggcggtaa aacgacgcat caggaaacgt tacaaaccag tcccgatgcc attcttaact
     5101 gcatgaccac tatcatcatc aacccggaac tgttcggcgc gccggattgt agcgcgcaga
     5161 ctgaagcctt tgccgagtgg gtgaaagcct cgccgcatga tgacgataag ccgattttgc
     5221 taccgggcga gtgggaagcg aacacgcgtc gcgaacggca gaagcagggg attccattgg
     5281 atgggggaag ctggcaggcc atttgtgatg cggcgcggca gattggtatg tcggaagaga
     5341 cgttgcaggc tttctgtcag cagttagcca gctaaaaaaa agcccgtcca gtggcggacg
     5401 ggcaaacaag ggtaacatag gatcaatgag ggttagagca tatgcgtctg tcggcaaaca
     5461 gacagggaaa tacttgtgct ggacgtagcg taaacgcctg atccgtccaa cggttcggat
     5521 tttgtaggcc tgataagacg cggtagcgtc gcatcaggca tctgttgccg gatgcggcgt
     5581 aaacgcctta tccgtcctac gaattactta taaattaccg cagtaccgct taatttattg
     5641 ttgttggtgg cggaagtgat gctatatcca ctggcaccgg ctgccgccgc tttctcagcc
     5701 agtttcgctt ccagggcatc gagggtggat gcgccatcgg cagaaaccac gccgatttta
     5761 ttcatgttct gtgcctggga tgccgttacc ggttccgccg cgaacacgcc aaatgacaga
     5821 gttgaaagag ccatagcagc aacaacagta ttgatagttt tcataattaa tctctcgcag
     5881 gtgatttttg tataagtgac gttgtttcgt cgatgtgatg agtatcacgt ttttttacga
     5941 gagataaaat cgaagagaat tgaccgcctt gttcaaataa attgattgat atctaacata
     6001 ctgataaacc atttatttgt tgtacttatg gacttgtttt atgcagcgtg gcagtcacag
     6061 cagcgttaaa ggcacggaat gccacgcaaa atacaaatta tactcgctat gcatctcgcc
     6121 agttgtagtt aacgtaactg gctgtctttc gaacctctgc gattatatcc tgtataagct
     6181 ggtttttgta aatctttctc ctgctgacaa tgaatgcata agcgcacgcc aggaatggct
     6241 tcccgacggg cttgcgggat gggggcaccg cactcttcac attcatacag gctttcgcca
     6301 cgcggaattt caccccgagc gcgggcaatc gcatcttcaa ttgtactgtt gatctgttcg
     6361 ttgacggcgt catcgttagc ccaaccggat gccatatcgt cctccccata tcaatgcttg
     6421 tacagttaag tgtagctaat atagggacga acctgggcag ttcaagcgtc agatctccga
     6481 ccattcccgc agcagattat gataaagatt gagcagcgac aggatctctt cattttcacc
     6541 atagcggctt ttcagcgact gaatattttt gtccagttca aacagcatgg cgcgcttttt
     6601 atcatcgcgg atcatcgact ggatccacat aaatgatgcc actcgtacgc cgcgggttac
     6661 gggtgtcacg caatgcaggc tgctggaggg atacaacacg agatcacctg ccgggagttt
     6721 tacccgatgt tgtccgaagg tgtcattaac gaccagttcg ccgccgtcgt agctttgtgg
     6781 atcgcttaaa aacagcgtgg cagaaaggtc agtgcgcatc cagccgtttt gtggatggct
     6841 gcgtactgcg ccatccacat gaaaaccata ggtttcattg ttctgatagc gattaaacag
     6901 cggcgtggaa agggtacgcg gcaaggccgc ggcaaagaac aaagcatgtt ggttaaccgc
     6961 gttcagcacc tcattttgca gggcggcgta taacgtgctg cgggtgtcta cctgttgatt
     7021 gttcttaact tgcgcacctt gtgcgccggt ggtgacgcgt ccatccaccc attcggcttg
     7081 ttccagttgt tcgcgaaaac gggcgacgtc ctgtggcgat aacacgccgg gaatgtggta
     7141 catcatacaa gttctccaaa aagtggggcc tgcgccccac atctgaatca gaaatgcata
     7201 ttggctgtga gcaagaaggt tcttggctcg cccgggtgat aacggtagcc gctcttattg
     7261 attgaggcga cgtaatcggt atcaaacagg ttgtagacgt ttagctggaa gtcgagattg
     7321 cgattaaccc gataccccag tttggcatcg gcgacccagt aaccttcggt aaacgctggc
     7381 gttcccaccg cgccgtctga acctttatgc atactgccga tatagcgtgc gcccgcgcca
     7441 acagagatat cttcggttgc ctgatattgg ctccataagg tgaaggcgtg ctccggggta
     7501 tacggcagcg atgaggaacc atcctgggca acatctttgc cgttgttgat ggttgctttt
     7561 tgctgggtat agccgccaat cacctgccac gcgggagtga tattccctgc cacggatatc
     7621 tcatagcctt cgacgcgttt cttaccgtat tgcgagtaag tcccgtcatc attttgctca
     7681 acttcatttt cgatatcagt acggaacagc gcggcggtga gtaacaggcg tttatccaga
     7741 acctgccatt tggtgccaat ctcgctggtg ttggcttttt gcggtttaaa atcggtgcgg
     7801 ttggcactgt taccgctgcc agactgcgca agggcgaagt tgttgccgcc cggaggctgc
     7861 tgggaaacgg catagttaat atagacattg ccgttttccg tcaggtgata cagcgccccg
     7921 gctttccagt tcaccagatt gcccgacttg gcggtgtcga cggtggtgac cggagaacct
     7981 tttgccacac cagctgggca ggtgatggca ccgcgtccgc tgccgccgca ggcggtggca
     8041 ctgtcatatt cagtatgata attatccaga cggatcccgc cgttcagctc aaaatcacgg
     8101 gtgatttgca gcgtatcgaa ggcgtaaatt gcgaaggtat ccgtctgacc attggcgttt
     8161 gcgccgttgc gcgtcaggcc gccgggatga atgctgctgt caggatgata aatatttacc
     8221 gccggtaacg tcaccggatt aacgccgtag ttagtctgcg tttcacgggt aaattccacg
     8281 ccggtactga catcatgacc gatagaaccg gtatagaacg tcgaggtcag gttggtctgg
     8341 ttggtgagaa ttttattact cacatctttg gtattcgccg tgcgtgacca cgtccagcta
     8401 ttcacatcgc tggtgggctg ggtaatattc gacgccccac ccataatcgc cgtcatcagg
     8461 taatcctgct tcacgcgcga ccaacgggta gtgttgcgaa tggtggtgtt atcgttgatg
     8521 tcgtgctcaa aacgcatggt ggcggtgtcg gtggtcgaat cgtcgtaatc ggaatccgtg
     8581 ccgtaaaagt tatgcgtatc aacttttccg gaatgattca gggtcgccgt tcctgcagat
     8641 ggggcagaat agcccggcaa accgatggtc ggaatgccgc cgtctggcgt gttgtgctga
     8701 gtgacatgca gataattaag atacaaacga ttcgctgtac caaggccaaa agcgacagaa
     8761 ggggcgacgc cgtaacgctc atttttgact ttgtcgcgtc cggcatcgtg cgttttttcg
     8821 cccatcacat tcaggcgcac cgcggtagta tcaccaatga cctgattgac gtccagcgtg
     8881 ccgcggcgga accaggcgct gccaatactg gcggaggcgt caatgccgga atcattgcgc
     8941 ggctgcttgc tgatcatatt gatcgagccc gtcggcgcgc tgcgcccgta gtcggtgccg
     9001 gacggccctt taatcacttc gacctgctcg gtattgaagg tgtcgcgcga gacgctgccg
     9061 atatcgcgga tgccatcaat ataaatactg ttagatgtat cagcaccgcg catataaatg
     9121 gcgtcgccag tggtggagtt accgttctca cccgcaaaaa acgcacccac gccggggacg
     9181 tttttgagtg cgtcggtaag gttggttgcg ccctgatctt taatcacttg ttcagaaatc
     9241 accgtcatcg tgcgggtagt atccgctacc ggacgcgaga atttcggatc tgcagattgt
     9301 tgtggcgcat aaagcgaagg cgttgatgct tcgacaacca gcgtgtcatc cgcgttagtt
     9361 tgcccttcgg cggcgagtgc ctgagccaca ggcgtgatgc caatacaaag accggcaaag
     9421 aacgtgagcg aatgaaattg tctggcaggg aaattgcgat tgttttccat tttgcaggtg
     9481 actttttctt atatgttgaa attttcactg tcacgagaaa gcctctctgc ggatgcgatg
     9541 ccactgttaa tcccgtgata gtgttcgtgc aagtgataat aattttgata agcattatca
     9601 cttggcgcga ttttctatca cccattgcgc gaaaaagaaa tacatttatt tacattacgt
     9661 tcatatttga tgttttttga ttacaaaaca ggcgaaaact gcatccagag gcgaatgcag
     9721 ttacgttggt ggggttattt gtagatggtt gcagtaccga acatctggtc atttccgcct
     9781 gcggaattaa tgacataacc tttcgcgcct tgttcgcgcg ctttttctgc cagtttatcc
     9841 tccagatcgc ttaggtttga tgcgccagtt gccgaaacgg taccggcggg ccgtagctga
     9901 ccgctgtcca ggttactcat cggttgcgcg gcataagccg tgagagagat tccgctcagg
     9961 acggtggcaa tcagtagtgt gaggcacttt ttcataatga catcctcata aatacaacgg
    10021 gtagtgcctg ataagtgtag gtcgcctggg cgatgaaaat gacgcaaaaa attgacagag
    10081 atacgcttat ttttttgaac gatatatttt tacaaaataa gacaaatcaa tgacatgcat
    10141 gatgctgatt tttatgatga attgccataa accatagcca tgttaaggtt tttagaaatg
    10201 ataaatgcag ccagatattt accctgtcgc gttatgcggg tatcttacgc cgctgcttaa
    10261 aggagaatgc tatgtccgcc cagaaaccgg ggttgcatcc gcgcaaccgt catcacagcc
    10321 gctacgatct cgccacgctt tgtcaggtca atcctgaact caggcaattc ctcacgctta
    10381 cacccgcagg ggagcaaagc gtagactttg ccaatccgct ggcggtgaag gcgctcaata
    10441 aggcgttgct ggcccatttt tacgccgtag cgaactggga tatcccggac ggttttctct
    10501 gcccgccagt accgggccgg gcggattata ttcatcacct tgccgattta ctggctgaag
    10561 caagcggaac aattccagct aatgccagca ttcttgatat cggcgttggt gcgaactgta
    10621 tttatccgct gattggcgta catgaatatg gctggcgttt taccggtagt gaaaccagca
    10681 gccaggcgtt aagcagcgcg caggcgatca tcagtgctaa tccggggctt aatcgcgcca
    10741 ttcgtctgcg tcggcaaaaa gagagtgggg cgatttttaa cggcatcatc cataaaaacg
    10801 agcaatacga cgcgatcttg tgtaacccgc cattccacga ttccgccgct gcggcgcggg
    10861 caggtagtga gcgtaaacgc cgtaacctgg ggctgaacaa agacgatgca ctgaactttg
    10921 gtggccagca acaggagttg tggtgtgaag gcggtgaagt cacctttatc aaaaagatga
    10981 ttgaagagag caaaggcttc gcgaagcagg tgatgtggtt tacttcactg gtatctcgtg
    11041 gtgaaaactt accgccgttg tatcgtgccc tgacggacgt gggcgcggtg aaggtggtta
    11101 aaaaagagat ggcccagggg caaaagcaga gtcgctttat tgcctggacc tttctgaacg
    11161 acgagcagcg ccgccgtttt gtcaatcgcc agcgttaaag cgttggctcc cccggcggca
    11221 gcggttcagt cggggtcgcg cccggagcag gcagcacctg ataagtctgc actggcgcgc
    11281 gaacgcccgc caggtcgaaa tgttttttca cctggctgtc gagggcaaag cgtaccgtcc
    11341 actgtttgag tggcagcgtg gtgaacgaaa cacgcagtgt aaacgcggta ttgcttaagc
    11401 cgacaatccc ggcaaagttc ggttcaccaa taaccagccc gcgaatttct tcgttttcca
    11461 ttaattccgc taccgcatct ttcagtgcct gattggcttt atcagcatct tcatggcgat
    11521 caacatcgta gtttgccact accgaaccaa tgccgcggac aaagttggca aaggtggtaa
    11581 tcgaagacca cggaatgatg tggtacgccc cggtatcctg gcgcacgccc acggagcgaa
    11641 tcgacatccg ttccactgtg ccggtcaacg gcccgatagt caccaaatct ccagtgttca
    11701 tgccgttttc aaactgaata aacaccccgg taatgatatc tttcaccagc gtttgcgaac
    11761 caaacgagat agccagacct aatgccccgg cacctgccag caatggcgcg atattgacgc
    11821 cgatttccga caacacaatc atgatggtga tggtactgat aatcaccgcc agcgcgttac
    11881 gaaacagcgt cagcagggta cgcgtacggg cgctgggtag cgggcggcca tgaatatccg
    11941 aagccagccg gttttcgatc aaactggcga gcactgtcca gccaaccgcc gagaagaaaa
    12001 gaatgagtgc gatacggatc aggatatcta cggttttctg ccccgcgccg ttttgcagcc
    12061 agttccagaa atcgaacaat ccccatgcgc tcaacagcaa cattaccgcc acgcagacgg
    12121 tcagaatacg cgccgttttc agcgccgccg aaagccagcc atttagccgt ttttgcagct
    12181 ccggatagtt acgctgagta tgtggcgaga gagtgatggt ttttgccagc cagcgggaaa
    12241 acataccgga aacaaacgct gcgataccaa taatcgccag gctgcgcacc gttgcaccca
    12301 tcataaattt caggctgttg cccggatcga acaacgaaaa gaaaaacagc acgataaaat
    12361 aggcgcttgc cagccagtgc cacaccagcg caaaggcgcg gataaacagg ctgaaaaagg
    12421 ccagcgaatg ctccgcgaag ttgagcaaat gctgggtaat ctctttttta ttacgaaaga
    12481 tcaggtacaa cgcccagact gtcatgcaca gcataatgat gacgttcgcc agcgcaccta
    12541 tctgtacatt cacctgatta gagataatcg gcacggccac aatcaggcca tagccaatca
    12601 ggctgcttaa ccagctcagg cgacgactcc agtaacgggc gctctcgtcc tgaatcgtga
    12661 acgggcgcag ttccgccacg tttgggcaaa aaatcaggcg tagtacagct ttgaaaaatt
    12721 caatcagggc aaaggcgttg agaaataaac tttgttggaa agcgatggta cgactgcccg
    12781 cattcaggtt gtcgcttaat acctggccga caaataatgt cagtgccagt aacagcaggt
    12841 cgataataaa cgccccgata atcatcgccg gaagctgcag ccagttgctg cgttcgcgat
    12901 ttttttgccg cgcccactga cccattttgc gatacagcgg cagtgcgcac aggcgaatca
    12961 gccagtaaaa accaaacact aaaaccgcta acattgaaaa atgggtcagc gcattgctga
    13021 aggtttgtgg attaaacggc ttatgcgggg agccggtgat attgcgataa agctgcccga
    13081 agcgggcgga aagggcctca ccataatggc ggctgaattc ggtgaccttt tgcagcacgg
    13141 tttgctcttc aaccagcgtc ggcggcacga tcttcgggac cggttcagca ggtggcgtcg
    13201 cggcgacggt gcgcaactgg ttgatcaact ctttacgcga ggtgtcatta tccagcacat
    13261 ccgccagtgc gccataggcc gctttttttt gttcgatatc cggttccggg gccggttcag
    13321 tcgttgagtc cgttgtcgtt gtggttgtaa cgccgggtat ggataccgcg tgggcaggtg
    13381 cgcccagcag gcagaagagg atgaacagga tccaccgcat gactcctcca gtgagaaaac
    13441 agagcaaaaa gataagtata gatgctgggg aaggggcgat tttaaatgag aggaatctgg
    13501 tgtgcctccc tttcgggtga aagggaggaa gggatattaa gcaggttgct gacaacgtgc
    13561 gggctttttt gccggatgcg gcgtgaacgc cttatccgtc ctacaagttc atgcaatttt
    13621 aataagttgc cagatatcct gtaggcctga taagcgtagc gcatcaggca atgtgtctta
    13681 ttaagagacg tgctgcaaaa attcctgcaa gcgctggctc ggcgggttct tgatcaacac
    13741 ctgcggatct ccatcttccg caatccggcc tttatcaatg aagatcaggc gcgaagctac
    13801 tttctcggca aaaccgattt cgtgggtcac gatcaccatc gtcatccctt cttcagccag
    13861 atcttgcata accttcagca cttcatggcg cagttccggg tcaagagcgg aagtcggttc
    13921 atcaaacagc atcattttcg gcttcaccgc cagcgcgcgg gcaatcgcca cacgctgctg
    13981 ttgaccacca gaaagttcgg aagggtagtg atgtgcacgt tctgccagac cgactttcgc
    14041 cagcagctca cgcgccagtt tttccgcctc ttctttgttc gcgccacgca cgcgtagcgg
    14101 gccaaacatg acgttttcca gcgccgtcag atgcgggaag aggtaaaact gctggaacac
    14161 catacctgct tcctggcgaa tcaggcgctc gtcaactttc ggatcgttaa ccttcaggcc
    14221 atcgacaatc agatcgccgg aggtgatttc ttccagtttg ttgatgcagc gcagcagggt
    14281 cgatttaccg gaaccggacg gcccgataat caccacgact tcgccctggg caatgttcaa
    14341 atcgatattg tgcagcacct gggttgggcc aaagtgcttg gagacgtttt taaattcaat
    14401 cacaggattt tcatccttct ttccagacga cgcagaataa agctcagcac cagggtaata
    14461 atcagataga acaccgccac ggcgctccag atctcaaggg cgcggaagtt accggcaata
    14521 atttcttgcc cctgacgggt cagttccgcc acgccgataa caataaacag cgaggtgtct
    14581 ttaatgctga tgatccactg gttacccagc ggcggcagca tacgacgcag tgccagcggt
    14641 aaaatgacgt agcgaatggt ttcccaacgt gaaagaccga gcgccagtcc tgcttcacga
    14701 aaacctttgt ggatagacag caccgcacca cgcgtgattt ccgcaatgta cgcgccggag
    14761 ttgatcatga tggtgaccac cgccgcagta aatgggtcga tgcgtaagtc gttaaacgcc
    14821 atcggcaggg cgaaataaat aaacatcacc tggacgacga taggtgtgcc gcggatcact
    14881 tcaataaaga ccagcgcgac gtggttggct atccaaccac cgaaggtgcg tgcaaaacct
    14941 gccagcaatc cgattaccag accgccagcc agaccgagga ccgatatcca cagggtcatt
    15001 ttggcacctt caatcagaag cggaatggca ggccagatag cactccagtc aaactgcata
    15061 tgttgttcct gttaccgtgg tgtaaataat caaattcagg gtaattaatg gccccggagc
    15121 gggtttgcgc ccctcaccct aatcctctcc ccagagggga gagggaactg ccagtgcgct
    15181 ttacaggcgt agcattatta tttcggttca gtaccgaacc attttttgta gatttcgttg
    15241 taagttccgt tctcgcgcag ggttttcaac gcgccgttga ctttgtcacg cagctcgtcg
    15301 ctacctttcg ggaacgcaat accgtattgc tgtgcttcca gagagtcgcc taccgctttg
    15361 aactgaccgt taccggcggt tttgatgaag tacagaatgt ttggcgtatc gtgcagaacg
    15421 gcgtctgcgc ggttggtgcc cagttccata taggcgttat cgatgttcgg gaactgacgc
    15481 agatctttag ttttgatgtt tgctttcgcg taatcaacgg agccagtgcc gctcttcacc
    15541 gcaaccactt tcccgtcgag atctttcacg cttttcacat cgttattgtt agctttcacc
    15601 atcactaaca ggccgctttt gtagtagccg tcagagaaat cgatcgcttt tttacgctcg
    15661 tcggtaatgg taatgcccgc cagcgccaga tcgacgtttt tggtttgcag tgccggaatg
    15721 atcccactga aatccatcgg cttcagttcg taatccagct tcagctcttt agcgatggca
    15781 gcccacagat caacgtcaaa gcccacatat ttatcgccct gtttaaattc aaacggaacg
    15841 aaggcggtat ccgtcgcgac aactaatttt ttatccgcgg catgagaaga aaccgcaaaa
    15901 gccagggtca gtgcagccag tgaaactttt aatacagact tcatagcatt tccttttttc
    15961 tatccacggg acgatcccct gcgtgacatt cacatatatg aaaaaatcgt gccagttttt
    16021 caactccttg atttttcgag atgcggcaaa aacgtgatgc acgatttata gggcaatacc
    16081 ctgaagatgc accattctgg ggcaccaaac tggtgcgcta aaattgtgca ctcaacacag
    16141 tgattattta gcgtaaaacc tgatgaaaaa acaatcttta tgtaacgatt gtgtgatgat
    16201 gtggatacaa aaaatttact taatcagctg gggatagcag atggatgcac taaataagtg
    16261 cgttgaggcg ggctgcaaaa caaaacggcc tcctgtcagg aagccgcttt taccgtgtac
    16321 taaagttctg caccatcagc gatggattta ttcgatgtta gactcgataa accacaggaa
    16381 tttatccagg tcgcgagacg cggcggtcag gatatctgcg gtgtcgtcat ctttcgcttc
    16441 gccaatcgct ttgcgtacgt cattagcgac gattgcgtaa cggtcagcca gttctttcag
    16501 gtgatcctga acgttgtgga tgtccagcgg gtaacttttc agcggggttt tgctgttgat
    16561 aacttgagtg gtccccagag ctacaccgcc tagctgcacg gcacgttctg ccatggtatc
    16621 cagatgatcg atcagtgcag tgcggaagcc atccagcatt tcatgtacgg caatgaagtt
    16681 agcgccgcgc atgttccagt gcgcttgttt ggtaatcaaa gaaagatcaa taaactggat
    16741 aacctggcga ttcagcaact ctactgttgc ttttttctcg ctgtcagaga catcgttgcg
    16801 ggtataaagc agattggtcg cttttgattt aactaattta gcggtactca taatttcata
    16861 tcctcttgat gttatgtccc agtaattaac gagactaagt atagcaccgg ctatgtgttc
    16921 cgctattctg gctgttccta tcacactaat agtggtaaca agcgtgaaaa
//
LOCUS       DAPGOF010000104        16825 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.105, whole genome shotgun sequence.
ACCESSION   DAPGOF010000104 DAPGOF010000000
VERSION     DAPGOF010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.105"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..575)
                     /locus_tag="RHV13_003004"
     CDS             complement(<1..575)
                     /locus_tag="RHV13_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP pyrophosphate phosphatase"
                     /protein_id="HDV1242518.1"
                     /translation="MKVSVPGMPATLLNMNNNDIYKMVSGDKIDVKMNIFQRLWETLR
                     HLFWSDKQTEAYKLLFNFVNKKAGNINASKYFTGAVDENEKEKFIHSLELFNELKTCA
                     KNPDEVVAKGKMRWVAETYGDIELSVTFFIENNEICTQTLQLHKGPGNLGVDLGEAYL
                     PGVDMRKCYFGKKTIIDNKVLYLEPGWNANL"
     gene            complement(870..1685)
                     /gene="djlA"
                     /locus_tag="RHV13_003005"
     CDS             complement(870..1685)
                     /gene="djlA"
                     /locus_tag="RHV13_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HDV1242519.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            1940..4294
                     /gene="lptD"
                     /locus_tag="RHV13_003006"
     CDS             1940..4294
                     /gene="lptD"
                     /locus_tag="RHV13_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HDV1242520.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            4347..5633
                     /gene="surA"
                     /locus_tag="RHV13_003007"
     CDS             4347..5633
                     /gene="surA"
                     /locus_tag="RHV13_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HDV1242521.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKIGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            5633..6622
                     /gene="pdxA"
                     /locus_tag="RHV13_003008"
     CDS             5633..6622
                     /gene="pdxA"
                     /locus_tag="RHV13_003008"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HDV1242522.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRSYSPNSPAQPQTAGTLTLLPVALRAPVTAGQLAVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPLLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            6619..7440
                     /gene="rsmA"
                     /locus_tag="RHV13_003009"
     CDS             6619..7440
                     /gene="rsmA"
                     /locus_tag="RHV13_003009"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDV1242523.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            7443..7820
                     /gene="apaG"
                     /locus_tag="RHV13_003010"
     CDS             7443..7820
                     /gene="apaG"
                     /locus_tag="RHV13_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HDV1242524.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            7827..8669
                     /gene="apaH"
                     /locus_tag="RHV13_003011"
     CDS             7827..8669
                     /gene="apaH"
                     /locus_tag="RHV13_003011"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HDV1242525.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDMGE
                     AAAS"
     gene            complement(8747..9226)
                     /gene="folA"
                     /locus_tag="RHV13_003012"
     CDS             complement(8747..9226)
                     /gene="folA"
                     /locus_tag="RHV13_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HDV1242526.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(9418..11280)
                     /gene="kefC"
                     /locus_tag="RHV13_003013"
     CDS             complement(9418..11280)
                     /gene="kefC"
                     /locus_tag="RHV13_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HDV1242527.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKIGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(11273..11803)
                     /gene="kefF"
                     /locus_tag="RHV13_003014"
     CDS             complement(11273..11803)
                     /gene="kefF"
                     /locus_tag="RHV13_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HDV1242528.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(11914..13245)
                     /locus_tag="RHV13_003015"
     CDS             complement(11914..13245)
                     /locus_tag="RHV13_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1242529.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(13303..13590)
                     /gene="fixX"
                     /locus_tag="RHV13_003016"
     CDS             complement(13303..13590)
                     /gene="fixX"
                     /locus_tag="RHV13_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HDV1242530.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(13587..14873)
                     /locus_tag="RHV13_003017"
     CDS             complement(13587..14873)
                     /locus_tag="RHV13_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDV1242531.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGTACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(14924..15865)
                     /locus_tag="RHV13_003018"
     CDS             complement(14924..15865)
                     /locus_tag="RHV13_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDV1242532.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINAFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYASVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFKYADYGIVGDAVKILPALTAALAR"
     gene            complement(15880..16650)
                     /locus_tag="RHV13_003019"
     CDS             complement(15880..16650)
                     /locus_tag="RHV13_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HDV1242533.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT         4144 a         4693 c         4166 g         3822 t
ORIGIN      
        1 tccaggttag cattccaccc gggttccaag taaaggactt tgttatctat tattgttttt
       61 ttaccaaagt aacacttcct catgtcaacg ccgggaaggt aagcctctcc aagatcaacg
      121 cccaagttac ctgggccttt atgcagctgc aacgtctgag tacatatctc attattttca
      181 atgaaaaaag tgacacttaa ctcgatatca ccgtaggtct cggcgaccca gcgcatcttg
      241 ccctttgcga ccacctcatc cggatttttt gcgcatgttt tgagttcatt gaataattcc
      301 agactatgga taaacttttc tttctcattc tcgtcgacag ccccagtaaa gtattttgag
      361 gcgttgatgt tgccagcctt tttattcacg aaattgaaca gaagtttata agcctcagtc
      421 tgtttatctg accagaacag atgacgtaac gtctcccaca agcgttgaaa gatattcatc
      481 ttcacgtcaa tcttgtcccc gctcaccatc ttataaatat cgttattgtt catatttaaa
      541 agcgtagccg gcatgcctgg aactgatact ttcatggtaa tagcctcgca ttgagttaaa
      601 aactcatgat gcgcgctctg aataaatata ctttcaaaaa tcgctcattc agatatgtgt
      661 ttttaatttt taagacgtaa aaatataatt ccgctcgtcg taaagctctc aaccttaagc
      721 agggtcaacg cgataaaacc aacagaaacc ctcccgtaaa ccgtgtattg agcctgtcca
      781 ccactctgag taaacgcctt ttctcaaaga agtgaccgcc agggcggcca cctttaatcg
      841 tgggcagtta ctcagcatca tttacagggt catttaaacc ctttctgctg ttttatcagc
      901 tcataggcct gctgaatttc ctgcgctttc tgcttcgcca tctccatcat ctctggcggt
      961 aaacctttcg ccaccaactt atcgggatgg tgttcactca tcagcttacg gtaggcacgt
     1021 ttgatggtgg tcgcatcatc cgtcggcttc acgcccaaaa cattacaggc atcttccagc
     1081 gtcgggccac gctgcgcttg ctgccagtta ccaccgccag attgctgctg atacccgccg
     1141 ccaaactgtg caccgccctg catcatgcgc aaaaactggt caaactgggc gcgggagatc
     1201 cctaattctt cagcaataac atacagcacc gcccgttcat tcgggtgcag tgaaccatca
     1261 gcaaacgccg cctgaatctg gatctccaga aacatacgaa ttaagtcaaa acgaccaaag
     1321 caaacactgc gaaactggcg catcttttcg cgcagcgggt aattgtctga ttttcccacc
     1381 cggaacgcgt tttgcgccgc agtacgggaa gcgccatgaa gattcattcg gtccatcaac
     1441 tggctggcaa tatgaatatc agcctccgtg acgcgacctt tggattttgt taaatgcccc
     1501 atcacttcaa aagtggtggc aaaaaacagc gcctgacgct cacgctggtt ggcaaaccac
     1561 gccattttac ggctacgggc tttatcaaac atatggccaa ttaacaggcc taacactacg
     1621 ccccaaaagc cgccgcccat cagtaaggcc acggccacgc caatgatttt tccccaatac
     1681 tgcatatatt ccccaaatcg acacacggat atcagggcta tctcccacaa tataaaggtg
     1741 cttttaccgt tttccggctg cggtcaattg tgggacatcg cctataattt gcattatcat
     1801 acctgtcatt caatgccgtg cctaacacca ctgacgctat tcggacagga ttaacactag
     1861 cgtagagatg acgagtacgt tagtctctga ccgtttgtca cgcgcaacgt taccgatgat
     1921 ggaacaataa aatcaacgta tgaaaaaacg tatccccact ctcctggcca ccatgattgc
     1981 caccgccctt tatagtcaac agggactggc agccgacctc gcctcacagt gcatgttggg
     2041 cgtgccaagc tatgaccgtc ctctggtaca gggcgatacc aatgacttac ccgtgactat
     2101 caatgctgac cacgcgaaag gggactaccc ggatgacgcc gtgtttactg gcagcgtgga
     2161 tatcatgcag ggtaacagcc gtctgcaggc cgacgaagtg cagctccatc aaaaagaggc
     2221 accaggacaa ccggagccgg tacgtaccgt tgatgcgctc ggtaatgtcc attacgacga
     2281 taaccaggtg atcctcaaag ggccgaaagg ctgggcgaat ctgaacacca aagataccaa
     2341 cgtctgggag ggtgattacc agatggtggg tcgccagggc cgcggtaaag cggacctgat
     2401 gaaacaacgt ggcgaaaacc gctataccat tctggataac ggtagcttta cctcctgtct
     2461 gccgggttct gacacctgga gcgtggtagg tagcgaaatt attcatgacc gcgaagaaca
     2521 agtggccgaa atctggaacg cccgttttaa agttggcccg gtgccaatct tctacagccc
     2581 ctatttgcag ttaccggtgg gtgacaaacg ccgctctggt ttcttgatcc cgaacgccaa
     2641 gtacaccacc accaactact ttgagttcta cctgccatat tactggaaca tcgcgccaaa
     2701 tatggatgcc accatcacgc cgcattatat gcatcgtcgt ggcaacatca tgtgggagaa
     2761 cgaattccgc tacctctccc aggcgggcgc tggcttgatg gaactggact atctgccttc
     2821 agataaagtc tatgaagatg aacacccgaa cgatgacagt tcacgtcgtt ggttattcta
     2881 ctggaaccac tccggggtca tggatcaggt gtggcgtttc aacgtcgact acaccaaggt
     2941 cagcgatcct agctacttca atgatttcga taacaagtac ggttccagta ctgacggcta
     3001 cgcaacgcaa aaattcagcg ttggctatgc agtgcaaaac ttcaatgcca ccgtttcaac
     3061 caagcagttc caggtcttca gtgaacagaa caccagtagc tactcggcag agccgcagtt
     3121 agacgttaac tactaccaga atgatgttgg tccgtttgat acgcgtattt acggccaggc
     3181 agtgcacttt gttaacacca gagacgacat gcctgaagca actcgtgttc acctggaacc
     3241 gaccatcaat ttgccgctct ctaataactg gggcagcatc aataccgaag cgaagttgct
     3301 ggcaacccat tatcagcaaa ccaatcttga ctggtataac tccagaaaca caaccaagct
     3361 ggacgaatcc gttaaccgcg taatgccgca attcaaagtt gacggcaaaa tggtctttga
     3421 acgcgatatg gaaatgctgg ctccgggtta tacccaaacg ctggaaccgc gcgcgcagta
     3481 tttgtacgtg ccgtatcgcg atcagagcga catctataac tacgactcgt ctctgctgca
     3541 atctgactac tctggcctgt tccgggaccg gacttacggc ggtcttgacc gtatagcctc
     3601 cgctaaccag gtgacgaccg gtgtcacatc tcgcatatat gatgatgctg ccgttgaacg
     3661 ttttaatatt tccgttggtc aaatctacta tttcacggag tctcgcactg gcgatgacaa
     3721 cataacatgg gagaatgacg acaaaacggg ctcactggtg tgggcaggcg atacttactg
     3781 gcgtatctcc gagcgttggg gattgcgtgg cgggattcag tacgatacac gtctggataa
     3841 cgtagcgacc agtaactcca gcattgaata ccgtcgggat gaagaccgtc tggtacagct
     3901 gaattaccgt tacgccagcc cggaatatat tcaggctacg ctgcctaagt actattccac
     3961 tgctgagcaa tataagaatg gtatttcgca ggtaggtgct gtcgccagct ggccaattgc
     4021 cgatcgttgg tccattgttg gggcctacta ctacgacacc aatgcgaaca agcaagctga
     4081 ctctatgtta ggtgtgcaat acagctcctg ctgctatgca attcgcgtcg gttacgagcg
     4141 gaagctgaac ggttgggata acgataaaca acatgcggta tatgacaacg caatcggctt
     4201 taacatcgaa cttcgcggcc tgagctccaa ttacggtctg ggtacgcaag agatgctgcg
     4261 ttcgaacatt ctgccgtatc aaaacacttt gtgatttgtt gatttaccac gtaatccgca
     4321 gtgcggttaa ttgaaatgga aaaagtatga agaactggaa aacgctgctt ctcggtatcg
     4381 ccatgatcgc gaataccagt ttcgctgccc cccaggtagt cgataaagtc gcagccgtcg
     4441 tcaataacgg cgtcgtgctg gaaagcgacg ttgatggatt aatgcagtcg gtaaaactga
     4501 acgctgctca ggcaaggcag caacttcctg atgacgcgac gctgcgccac caaatcatgg
     4561 aacgtttgat catggatcaa attatcctgc agatggggca gaaaatcgga gtgaaaatct
     4621 ccgatgagca gctggatcag gcgattgcta acatcgcgaa acagaacaac atgacgctgg
     4681 atcagatgcg cagccgtctg gcctacgatg gtctgaacta caacacctat cgtaaccaga
     4741 tccgcaaaga gatgattatc tctgaagtgc gtaacaacga ggtgcgtcgt cgcatcacca
     4801 tcctgccgca ggaagtcgaa tccctggcgc agcaggtggg taaccaaaac gacgccagca
     4861 ctgagctgaa cctgagccac atcctgatcc cgctgccgga aaacccgacc tctgatcagg
     4921 tgaacgaagc ggaaagccag gcgcgcgcca ttgtcgatca ggcgcgtaac ggcgctgatt
     4981 tcggtaagct ggcgattgct cattctgccg accagcaggc gctgaacggc ggccagatgg
     5041 gctggggccg tattcaggag ttgccgggga tcttcgccca ggcattaagc accgcgaaga
     5101 aaggcgacat tgttggcccg attcgttcag gcgttggctt ccatattctg aaagttaacg
     5161 acctgcgcgg cgaaagcaaa aatatctcgg tgaccgaagt tcatgctcgc catattctgc
     5221 tgaaaccgtc gccgatcatg actgacgaac aggcccgcgt gaaactggag caaattgctg
     5281 ctgatatcaa gagtggtaaa acgacttttg ctgccgcagc gaaagagttc tcgcaggatc
     5341 caggttctgc taaccagggc ggcgatctcg gctgggctac accagatatt ttcgatccag
     5401 ccttccgtga cgccctgacc cgcctgaaca aaggtcaaat gagtgcaccg gttcactctt
     5461 cattcggctg gcatttaatc gaactgctgg atacccgtaa tgtcgataaa accgacgctg
     5521 cgcagaaaga tcgtgcatac cgcatgctga tgaaccgtaa gttctcggaa gaagcagcaa
     5581 gctggatgca ggaacaacgt gccagcgcct acgttaaaat cctgagcaac taatggttaa
     5641 aacccaacgt gttgtgatca ctcccggcga gcccgccggg attggcccgg acttagttgt
     5701 ccagcttgca cagcgtgagt ggccggtcga actggttgtt tgtgccgatg ccactctcct
     5761 taccgaccgg gcagcgatgc tcggtttgcc gctcaccctc cgttcttatt cccccaactc
     5821 ccctgcacaa ccgcaaactg cgggcacatt aacgctactt cctgtcgcgc tacgtgcacc
     5881 tgtcactgcg gggcagttag cggttgaaaa tgggcattac gtggtggaga cgctggcgcg
     5941 agcgtgcgat ggttgtctga acggcgaatt cgctgcgctg atcacaggcc ccgtgcataa
     6001 aggcgtcatt aacgacgcag gcattccgtt taccggtcat accgagtttt tcgaagagcg
     6061 ttcgcaggcg aaaaaagtgg tgatgatgct ggcgaccgaa gaacttcgcg tggcgctggc
     6121 aacgacgcat ttaccgctgc gcgatatcgc agatgctatc acccctgcac ttttgcacga
     6181 agtgattgct attttgcatc acgatttgcg gaccaaattt ggtattgccg aaccgcgcat
     6241 tctggtctgc ggccttaatc cgcacgcggg agaaggcggt catatgggta cggaagagat
     6301 agacaccatt attccgctgc tcgacgagct gcgggcgcag gggatgaaac tcaacgggcc
     6361 gctgcctgca gataccctgt ttcagccgaa atatcttgat aacgccgacg ccgtgctggc
     6421 gatgtaccac gatcagggtc ttcccgtgct aaaataccag ggcttcgggc gcggtgtgaa
     6481 cattacgctg ggcctgccct ttattcgcac atcagtggac cacggcaccg cgcttgaact
     6541 ggcgggacgt ggcgaagccg atgtcggcag ttttattacg gcgcttaatc tcgccatcaa
     6601 aatgattgtt aacacccaat gaataatcga gtccaccagg gccacttagc ccgtaaacgc
     6661 ttcgggcaaa actttctcaa cgatcagttc gtgatcgaca gtattgtttc tgccattaac
     6721 ccgcaaaagg gccaggcgat ggtcgaaatc ggccccggtc tggcggcatt gaccgaaccg
     6781 gtcggcgaac gtctggacca gctgacggtc atcgaacttg accgcgatct ggcggcacgt
     6841 ctgcaaacgc atccattctt aggcccgaaa ctgacaattt atcagcagga tgcgatgacc
     6901 tttaactttg gcgaactggc cgagaaaatg ggtcagccgc tgcgtgtttt cggcaacctg
     6961 ccttataaca tctccacgcc gttgatgttc catctgttta gctatactga tgccattgcc
     7021 gacatgcact ttatgttgca aaaagaggtg gtgaatcgtc tggttgcagg accgaacagc
     7081 aaagcgtatg gtcgattaag cgtcatggcg caatactatt gcaatgtgat cccggtactg
     7141 gaagtaccgc cgtcagcctt tacaccacca cccaaagtgg attccgccgt cgtgcgcctg
     7201 gttcctcatg caacgatgcc tcacccggtt aaagatgttc gtgtgttgag ccgcatcacc
     7261 accgaagcct ttaaccagcg tcgtaaaacc attcgtaaca gcctcggcaa cctgtttagc
     7321 gtcgaggtgt taacgggaat ggggatcgac ccggcgatgc gagcggaaaa tatctctgtc
     7381 gcgcaatatt gccagatggc gaactatctg gcggagaacg cgcctttgca ggagagttaa
     7441 cgatgatcaa ttcgccccga gtgtgtattc aggttcaaag cgtctacatt gaggctcaat
     7501 cttcacctga taatgaacgt tacgtttttg cttataccgt aaccatacgc aatctggggc
     7561 gagcgccagt gcagttgttg gggcgttact ggctgatcac caatggcaat ggccgtgaaa
     7621 ccgaagtcca gggcgaagga gtggttggcg tccagccact tatcgcgcct ggcgaagagt
     7681 accagtacac cagcggtgca atcattgaaa ccccgctggg caccatgcag ggtcactacg
     7741 aaatgatcga tgaaaatggc gtccctttca gcatcgacat tcccgtattc cgactcgccg
     7801 ttcccacact cattcattaa aagaatatgg cgacatacct tattggcgac gttcatggtt
     7861 gttacgatga actgatcgca ttgctgcata aagtagaatt tacccctggg aaagataccc
     7921 tctggctgac gggcgatctg gtcgcgcgcg gcccgggttc gctggatgtt ttgcgctatg
     7981 tgaaatcctt aggcgacagc gtacgtctgg tgctgggcaa tcacgatctg catctgctgg
     8041 cggtatttgc cgggatcagc cgcaataaac cgaaagatcg cctgacaccg ctgctggaag
     8101 cgccggatgc cgacgagctg cttaactggc tgcgtcgcca gcctttgctg caaatcgacg
     8161 aagagaaaaa gttggtgatg gcccacgcag ggatcacgcc gcagtgggat ctgcagaccg
     8221 ccaaagagtg cgcgcgcgat gtagaagcgg tgctgtcgag tgactcctat cccttctttc
     8281 tcgacgctat gtacggcgat atgccaaata actggtcacc ggaattgcgg gggctgggaa
     8341 gattgcgttt tatcaccaac gcctttaccc gtatgcgttt ttgcttcccg aacggtcaac
     8401 tggatatgta cagcaaagaa tcgccggaag aggcccctgc cccactgaaa ccgtggtttg
     8461 cgattcctgg ccctgtcgct gaagaataca gcatcgcctt tggtcactgg gcatcgctgg
     8521 aaggcaaagg tacgccggaa ggtatttacg cgctggatac tggctgctgc tggggtggta
     8581 cattaacctg cctgcgctgg gaagataaac agtattttgt ccagccgtcg aaccggcata
     8641 aggatatggg cgaagcggcg gcgtcttaaa cacagcctga tacaggaagg ccggataaga
     8701 cgcgaccggc gtcgcatccg gcactatccg taaattctat acaaaattac cgccgctcca
     8761 gaatctcaaa gcaatagctg tgagagttct gcgcatcggc atcgtggaat tcgctgaata
     8821 ccgattccca gtcatccggc tcgtaatccg ggaaatgggt gtcgccttcc acttctgcgt
     8881 cgatatgcgt cagatacagt ttttgcgctt ttggcaagaa ctgttcataa acgcgaccgc
     8941 cgccaatcac catgatttct ggtacgtcac cacacgccgc aatggcttca tccaccgatt
     9001 tcacccacgt tacgcgatcg tccgtacccg gttgactgct gaggataata tttttgcgtc
     9061 ctggcaacgg acgaccgatg gattcccagg tatggcggcc cataataacg ggtttattta
     9121 aggtgttgcg tttaaaccag gcgagatcgg caggcaggtt ccacggcatg gcgttttcca
     9181 tgccgataac gcgatctacc gctaacgccg caatcagact gatcattgag atttcccgat
     9241 aaaaaaaatt gtcgccacta tacgtaaagc gtaaaccgtc gtcgactggt gcgaggatga
     9301 tgttgaggaa aattttatat tctgctggcg agtccacgct ctccccctgg actcgccgca
     9361 ttacaatgaa acaaaaacaa acagttagct gtaaagtgtg atttacgtca ctctttatta
     9421 ggatgagggt ttcgtttccg gttcatccgc catgttgccg gtatgtttac cttcttctgt
     9481 tccctgccag ccatgtcgtt gaattaatga cagatgttcg cggtcctcgg tgatgatctc
     9541 acttaacatc gcgctggtgc gtttatagac cgccgcgcgg gctttggtgt cattctcaac
     9601 cattgccatc tcttccacca tctgaatatt aaagcggcgg aacacatcgg cacgttctcg
     9661 cgcttcatac ggccccagac ctaaactttc cagcgccaga cgcccgattt tcagcgcacc
     9721 ttcaaaggtt tcacgctccg gcttttcaac gcctgcctga cgcaaacgaa tgtagtggtc
     9781 gacatcgcgg gcgcgggcaa taatctgcaa atgcgggaaa tgttctttca ccatctccgt
     9841 cagttgcagg ttggtttgcg gatcgtcaat agcgttaatc agcacttccg ctttcgccgc
     9901 tccggcagat tccagtaaat ccatccgcgt ggcatcgcca taaaacactt tcataccaaa
     9961 tttacgcagc gtttcgatat ggtccggatc gtgatcgagc accaccattt tcaccccgct
    10021 ggagagcagt aaacgtccgg taatctgtcc aaaacgaccg aatccggcaa tgattacccg
    10081 cggctgttct tcgtcgatct catcggcttc acgcgcttcc tcagtagaag attgctcaag
    10141 gcgattgagg atcaccagca gaatcggcgt tgctgccatc gacagcgcca ccgccagggt
    10201 cagcgatttc gcccactccg gctccagcac attcgccatt tgcgctgcgc caaacaccac
    10261 aaaggcaaac tcactgccct gccctaacaa caccgcaaac caacgacgct gtttatttgg
    10321 cacttgcaac ggtcgggcaa tcagccacag catggcgatt ttgatgatga ggaaaccgag
    10381 cagcaaaatg acaatgcgca atgggttttc aagcagcgtg ccaaagtcta tcgacatgcc
    10441 aacaccgatg aaaaacagcc ccaacagcaa acctttaaat ggttcgatat cgctctccag
    10501 cgcatgacgg tattccgagc ttgccagcag tacgcccgcc agaaacgcgc ccatcgccat
    10561 cgacaagccg acctcttcca gcagcaaacc aaagccaaac acaaggaata acgccacggc
    10621 actaaacact tcccgcaagc cagagcgggc aacaaaacgc agcgccggac gcgtaacata
    10681 gcgccccagc aataccacca gtaccagcgc acccgccact tttaacgccg agagagcaaa
    10741 tgcgcccatc gtcgtcgagg cactgctcgc cgccagtagc ggaatcatcg ccaccagcgg
    10801 gatcgccgcg atatcctgga acagcagcac cgcaaaggcg ctgcgaccca tttgcgtcac
    10861 catcagattg cgttcattca tcgcctgcat ggcaatcgcc gtagaggaga gcgccagcgt
    10921 catgccgatc aattccgcga cctgccagcg cagcccaagt aacatgcaga acagccccag
    10981 caggccgccg caaatcacca tctgcaatgc gccgccgccg aacactgccg cacgcagctt
    11041 ccacagcctt tgtggatcga gttcgaggcc gataataaac agcatcagca ccaccccaat
    11101 ctcggcaaag tgcagaatag attcggcatc ggtcaccagc cgcaaccccc acgggccaat
    11161 aatgcagcct gcaatcaggt agccaagtac tgatcccaga ccgaggcgta ccgcaatggg
    11221 tacaatcagc gccgccgatc cgaggtagat cagcgcctga accagcgtat gactatccat
    11281 gatgtgcctc ctgccattcc agcagacgtt gcttatagtg gcgcgcctgc ccttcgaggg
    11341 tttcgtcgtc acaaataaag gtacagtgca tggcaaacgg tggcagccag ttcagcccgc
    11401 agtagattgc cgtcgcctgt agcggctgcg acagcacatc aaagcccgga tgcgcaccaa
    11461 tttcaaaatg gctttccccg ccgccggtcg tcaccgccca cagcaaatgt ttgccatgca
    11521 gcgccgtgcc gccgtgaccg taagcccagc cgtgcgacaa aactttatcg atccaaagtt
    11581 taagaagcgg aggaatgctg taccactgca tcgggtgctg ccagacgatc agatcggcgc
    11641 gagagagcgc ctcctgctcg gcggcaatat cgatattgaa gtcaggatag agttgataaa
    11701 gagagcgaat ttcgacgcct tccagcgtcc ttgcctgttc aagcatccgt ttattcgcat
    11761 gggaatgatg cggatacgga tgcgcataaa tgatgaggat catagttatt agcccgcgtg
    11821 tttacgcctt tgttttccca caaagtgtag tcatatattc acgcggctta tagtcaataa
    11881 tattgattca gaaaagagag aaatctgaac aaattatccc attctctgcc cgcggatcgt
    11941 catattgctg gtctgcgcca gtgacattcc tcgagtctcc ggggcaaacg ctacggaaat
    12001 caacaagcca aacagcgaga tacccgcccc cattagcatc gtgttactga taccgtaatt
    12061 attgataaat atcggcagtg cccaggtcga aacaatggtg ccaatacgac ttaaggacat
    12121 aatcacgccc acggcagagg cgcggatatc cgtcgggaag agttcattag gatagagcca
    12181 ctgcaaatta cccggcccgc cagagaaaaa ggcatacacc gcaaaggcca tcactaccag
    12241 ccagatcccc atatccggga ttagccccaa aaccgccagc gccagcgtca tcatggcaaa
    12301 gctgccaatc aacaatggac gccgtccggc agtgtttagc cacagcatcg gcggaataca
    12361 gccgagcata aagaacaggc taatcaccac attccccagt gccgcgtttt tgccaacccc
    12421 caatcccaac aaaccaacga tttgcgggcc aaaggtgtaa atggcgaaca ttgggatcac
    12481 ctggcaggtc cagatggcgg caacaaacag aacaaaagga aaatggcggc gattaaacag
    12541 atcgcgaaaa cgggtttgct gcggctgctc ttcatcgaaa gccaccggtt cgccaaacag
    12601 ttttatcatc atctcttcgc actcttttac tcgccctttg cgtaataacc agcggggaga
    12661 ttcaggcagt tcgaatcgac caatcaaaat caacaaacag gggatcgctg cgctacccag
    12721 catccagcgc cagccgcctt ccacatcata aagccagtag ccgaccagat cggcgcaggt
    12781 cgcgccgaca taccacatgg cggcgataaa gctgatggaa aatgcccgct gacgggtact
    12841 ggagaactcg gtgatcattg aggtggcgat gggataatct gcaccgatga caatgccgat
    12901 aagtacccgc atcaccaaca gttcgacggg ggatgaaaca aacatcgtcg ccaccgatat
    12961 cacgccgatg gcgatgatat caatgaggaa cattttgcgc cgtccgactt tatcggaaat
    13021 atagccaaac agcgatgtgc caacgaacag cccggcgagc gttcccgcgc ccaacaagcc
    13081 aatccagtca gcgtccagtt ttagcgccgg agtcagttgc tccagcgcca cgccaatcat
    13141 taccagtaca taaccatcca gaaacggacc accgcttccc cacagcaaaa tgcggcggtg
    13201 aatagaggag aatttgagat cgtcaaagtt tctggacggt tgcatgggtt attcctgttt
    13261 tttgcaatga gagtcagtgc ggcgttacgt atcaaaccaa catcagccgt aacggaactc
    13321 cacaccaaag gtgccgcgcg gatattccca ctgttccagc gccgacccca gccccagaat
    13381 gcgacaggtg ccgcactcca gacatccggc gtaatcgaag cgcacactgc cgtcatcctg
    13441 cttcttgtac agacctgcgg ggcacgcttt caccagcagc tccagggcct gtttatcggc
    13501 atcggccttc acaacaatgt gtggatgctc ttcatcgaca ttgaatttat tgacgcccag
    13561 tttgacgtcc acattgacgg gagaagtcat aaaacggtca ctcctttcat gccatccttg
    13621 atcagattga tgaagcccac tttcttgccg tggcggagga ttttcttgcg catcagttcc
    13681 ggcgcactgc cgtcaatggt gaacaggtca cgcgccacgc ccaccgccag ttctgggtag
    13741 ccgctaaaca tgcgtgggtt atcaaggaac gccggtagtt tctggtacat gcgcatatcg
    13801 cgcagcgggc cactctccag atgctgacga tattccgcga gtttttgctt actgaaatcg
    13861 tcgcttttca tcgctgaaag cacggttttt gctgcagctt ctccggcggc aatcgccaga
    13921 tccataccgc gaatggtaaa accgaggttc atacacattc cggcggcatc accggcaatc
    13981 aatacgccgt caccgaccag ctccggcagc atgttgatgc ctgcttccgg cactacgtga
    14041 gcggaatatt ccaccagctt gccacccgcg atcagcggtg caacggccgg atgttgtttg
    14101 aaatcttcca gcatttgcgg caccgatttt tttgcgtcat gcagatgatg cagaccacaa
    14161 accagcccca gcgacagggt attttcattg gtataaagga agccgccgcc catcaagcca
    14221 tcggtgggcg atcccgcaaa caggcaagcc gtaccctgat taccctgcaa ctgaaaacgg
    14281 tcttcgatta ccgacttcgg taactcgatc agttccttca cgccaaccgc cacatccgtc
    14341 ggtttaacgc gttttgccat ccccaacttt tcggcaagga tggagttcac cccatcagca
    14401 aggatcaccg ttttcgcttc aatcacatcg ccatcggctt ctacaccgac gactttgcca
    14461 tcgcgctgta cgaggttatc gacgcggatc ccggtaatta actgcgcgcc cgcttcttcg
    14521 gcctgctcca tcaaccaggc atcaaattta ctgcgtaaaa cggagtaaga acgctgggat
    14581 ggcgaggttt cgtcaccgtt gcagtagtcc atagtcatcg ctgacttttc cgtcataaac
    14641 gcgagttttt catgggtgat caggcgttct acgggggcgg attcggcgaa acctggaata
    14701 atgtgttcca gactgtgggc atagagacgc ccgccggtga cgttcttggc acctgcggaa
    14761 ttgccacgct cgataactaa cacttgcgcc ccttcgcggg cgagcaccag tgcggcaacc
    14821 gaaccggcaa gcccagcacc gacgatgatg gcgtcaaaga tatcttcgga cataagagct
    14881 ccctgtcagc ggcagggcca aaatgcagcc ctgccagagt ggatcaacgc gctaaagctg
    14941 cggtcagcgc cggaaggatc ttcacggcgt caccaacaat gccgtaatcc gcgtacttga
    15001 agatcggcgc atttttatct ttattgatgg cgaaaatggt ttgcgatgca ttagcaccaa
    15061 ccatgtgctg gatctgcccg gagatcccca ccgccaggta cagttcaggt ttcagcatca
    15121 ggttggagat accgacatag cgttcgtgct ccatccattt ttcgttttcc gccaccggac
    15181 gagaacaggc caactccgca cctatcgcct tgcaaagctg ttctgccagc gcaatgttct
    15241 ctttgctgcc aataccgcga ccgacgctga ccaccagacg ggctttgtcg agatcgacgc
    15301 tgttgctctg gcgcgcctgg gttgccgtgc gggtaatcgc cacagccgga gcctgccact
    15361 ccacggtgtg cgtttcgcca gtgcgtgacg cgtctggctg agccgcatcg aacgtgccac
    15421 tgctgatggt cagtaccgca tacggcgtgg caatgcgttc ttcgccaatc gccaggccgc
    15481 cgtaaaccat gtgtttcact gttgctttac cgtcctgtac gctgacggtg ctggcatcgt
    15541 tagacaccgc cgctttaagg cgataaccca gttttgccgc cagtaatttg ccgcgacggg
    15601 tgtttggcaa cagcaccagg ccgtctgcgc cgtgctggcg aatagtgtca gccatgacac
    15661 tggcgtaatc ttcgatcatc cgatcgtccg gtttgccgtt taatttccag acatgattag
    15721 cgccgagctg gattgcctgt gcgccgtcgg catcattgag gacaaaggca ttgatttgat
    15781 tagctaaagc ctgcgcaccg ttcatcagtt ccggcagacg agaaggggta tcgctgaata
    15841 cccagacttg agaaaatgtg ttcatagcat cccctgtaat taaatgactt tgcgaagatt
    15901 ttcagcaaat gcggcgatct gttcttcgcc gtcgccttca atcacgatgc gctgacgctc
    15961 gcgctgtttc ggcgcggcaa cctgttgttc agaccaggcg tccacggcgt taaaaccaat
    16021 atccgccgcc gaccatacct ggactggctt tttcgccgcg ccgagaatgg ctttcatcga
    16081 aggaatttgt ggggagttga tatcagtgga aacagcaaca accgcaggca gcggaatgct
    16141 taacgtttct gtttcatctt ccagttcgcg ctcaacggtg agggtatctg ccgtaaggga
    16201 gataattttg ctgacgccgt taactgccgg aatattgagg atttcgccca ccagcagacc
    16261 aacctgctgg gcataaaggt cggaagaacc atcgccacag aggatcagat caaagcctgc
    16321 tttctgggcg gctgcagcca gtgcgctcgc cgtttgttgc ggcagtgcct gctcgaactg
    16381 gtcatcaatc accacaatca gttcatccgg gccgcgcgat agcacatctt tacgcccttt
    16441 ggcgttggtc agggctttac cgcccacact taaggccgtc acctgcgcct ctgctgcctg
    16501 ttgctttagc tggcaagccg cttcaatagc gttgagatcg tattggctta ttttggcatc
    16561 ggctttgctg aagtctaatg aaccatcagc attattgacc gcaatatcct gttcatcagg
    16621 cacgcactta tagcaagtaa taatcttcat tgcatctcca gaaatcatga aggaaacgtc
    16681 gttcatatcg gtggtgttaa ttacagatat cacgcccttt aatttggaaa taaaacttta
    16741 atcaccaata ttgaaaatgt cacacgcaga taaatccaac tttcaatatt gttaagctcc
    16801 tcaccaatat tgaaaacatg gcgca
//
LOCUS       DAPGOF010000105        16551 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.106, whole genome shotgun sequence.
ACCESSION   DAPGOF010000105 DAPGOF010000000
VERSION     DAPGOF010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.106"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(79..231)
                     /locus_tag="RHV13_003020"
     CDS             complement(79..231)
                     /locus_tag="RHV13_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HDV1242534.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(496..1230)
                     /gene="cysH"
                     /locus_tag="RHV13_003021"
     CDS             complement(496..1230)
                     /gene="cysH"
                     /locus_tag="RHV13_003021"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HDV1242535.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAQLEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKMLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(1304..3016)
                     /gene="cysI"
                     /locus_tag="RHV13_003022"
     CDS             complement(1304..3016)
                     /gene="cysI"
                     /locus_tag="RHV13_003022"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HDV1242536.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGNLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(3016..4815)
                     /gene="cysJ"
                     /locus_tag="RHV13_003023"
     CDS             complement(3016..4815)
                     /gene="cysJ"
                     /locus_tag="RHV13_003023"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HDV1242537.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLTAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGELRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANHMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5133..5495
                     /gene="queD"
                     /locus_tag="RHV13_003024"
     CDS             5133..5495
                     /gene="queD"
                     /locus_tag="RHV13_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HDV1242538.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5573..6844
                     /locus_tag="RHV13_003025"
     CDS             5573..6844
                     /locus_tag="RHV13_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDV1242539.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGVTTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLEKNEGAALLFSGRICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6835..7095
                     /locus_tag="RHV13_003026"
     CDS             6835..7095
                     /locus_tag="RHV13_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HDV1242540.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7112..7687
                     /locus_tag="RHV13_003027"
     CDS             7112..7687
                     /locus_tag="RHV13_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HDV1242541.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7835..8695)
                     /locus_tag="RHV13_003028"
     CDS             complement(7835..8695)
                     /locus_tag="RHV13_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDV1242542.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSQARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQVDVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8692..9471)
                     /locus_tag="RHV13_003029"
     CDS             complement(8692..9471)
                     /locus_tag="RHV13_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDV1242543.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWRAAAQGNSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLCCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9449..10858)
                     /locus_tag="RHV13_003030"
     CDS             complement(9449..10858)
                     /locus_tag="RHV13_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1242544.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGVLLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10880..12334)
                     /locus_tag="RHV13_003031"
     CDS             complement(10880..12334)
                     /locus_tag="RHV13_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDV1242545.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLYDVEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNAGTIYPIEK"
     gene            complement(12404..13189)
                     /locus_tag="RHV13_003032"
     CDS             complement(12404..13189)
                     /locus_tag="RHV13_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV1242546.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     IFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKSYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDGGYLVR"
     gene            13508..14785
                     /locus_tag="RHV13_003033"
     CDS             13508..14785
                     /locus_tag="RHV13_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1242547.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSTSLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLIILLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAIASSK"
     gene            14812..16290
                     /locus_tag="RHV13_003034"
     CDS             14812..16290
                     /locus_tag="RHV13_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDV1242548.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMEKFNIPRHMLFDVQMPGTVLGQITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPETLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDAKAQDLSPEDLLNKKASCVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFMPVESNAKRY
                     DAMNKGIFKALTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
BASE COUNT         3930 a         4369 c         4179 g         4073 t
ORIGIN      
        1 aaatataaat tttaaaaaag ggtgcaggag accaaaccat cacaattcag tacgcggggt
       61 tttacccccg cgtggtggct acttcttcga ttcgtaagcg agaactgcct taaactccat
      121 accacgttct ctgatactca actcacacaa cgagtcacct accatcagcg tgaatcccag
      181 tactgtacaa cacagtacga tgattgccac aagggcatat tttgtcagca tgtcttgcct
      241 ccctgcaaag aagagactaa cattcgaatt gtttgggttc gaacgtaagc ctcaggttga
      301 tagaaatatc gcctggggct tttgtccatc tggaacctcg cgaatgctta acgccagaca
      361 gcctcaagca cccgacgcca ttctatacct gataattctt ccagcggcgt tcacgtttaa
      421 aatcaggaat gctgctcgca aaataaaaac gcccccatca tttctgacag aggcgtttaa
      481 tttatccggc aatatttacc cttcgtgtaa cccacattcc ctttttaagc caaagaaacg
      541 cgtttcttct tccgccatgc cgggttccca tttacgggtt gtatgggtat cgcccaccga
      601 taaatatcct tcgtcccata atgggtgata tttcaggcca tgcttttgca gatactgata
      661 tatcgttcgg ttatcccagt cgataatcgg cagcatttta aatacgccac gctgaattgc
      721 cagcaccggt aaattggcgc ggctgccgga ttgttcgcgg cgcaggccag caaaccacgt
      781 ttgcgcgtta agttctttca gcgcccggtt catcggttcg actttgttga tgtcattgta
      841 cttttcaatg ccttcaacgc cctgctccca cagttttccg tagcgtgctt cctgccaggc
      901 tgcgctttcg gtagcacggt acactttcag gttgagcttg agtttgtccg ttaactcgtc
      961 aataaagcgg taggtttccg ggaacaagta accggtatcg gtgaggatca ctggaatatc
     1021 cgggcgaatc tgattcacca gatgcaggct caccgccgcc tgaatgccga agctggaaga
     1081 aagcacatat tcaccgggca gattatccag cgcccaggct acgcggcctt cagcgtccag
     1141 tttttccagt tgggcgttag tttccgccag cgccagaatg cgatctacct tcggcagttc
     1201 gttcagggcg tttagatcga gtttggacat agattcctca ctgtttgcct tgcctgatgc
     1261 gacgctcacg tgtcttatca ggcctacaag accgggctga tggttaatcc cacaaatcgc
     1321 gcgccggatc gagcaccggg cgaatgatgc ccgcacgcac cgtaaagtcg ccgaagcctt
     1381 cacccgcttc gcgctctttc gcccagcgcc ctatcagttc atcaagcgac gccaggattt
     1441 ccggctcggt gatgttttct ttatacatcc gtgggatacg tgtcccaata cggttgccgc
     1501 caagatgcag gttgtagcga cccggcgctt tacccaccag gcccacttcc gccagcatcg
     1561 cgcgaccaca accgttcggg cagcctgtta cacgcatcac gatatgctca tcgctgacac
     1621 catgtttcgc cattaaatta tcgatgttgt cgataaaaga cggcaggaaa cgctccgctt
     1681 ccgccatcgc cagcgggcaa gtcgggaacg agacgcaggc cattgagttt tcacgctgcg
     1741 gcgtgacggc attcattaac ccgctctctt tggcgatctt ctcgatcttc gctttctcgc
     1801 tttccggcac accggcgatg atcagattct ggttcgccgt aatgcggaaa tcacctttgt
     1861 ggatcttcgc gatctccagc aggccggttt tcagcggacg ccccggataa tcaaggatgc
     1921 gaccattttc gataaacagc gtcaggtgcc agttatcatc aatgccctta acccagccaa
     1981 tacgatctcc gcgtccggta aactcatacg ggcgaatcgg ctcgaacttg atccccgcgc
     2041 gacgctccac ttccgcttta aacgtctcaa cccccacgcg ctccagcgtg tatttggttt
     2101 tggcattttt acgatcggtt cggttacccc agtcacgctg agttgtcacg actgcttccg
     2161 ccactgccag cgtatgctcc agcggcagat agccaaactc actcgcggtg cgggcgtagg
     2221 ttttcttgtt gccgtgttcg atggaaagcc caccgcccac cagcaggtta aagcccacca
     2281 ggttgccgtt ttcggcgatc gccacgaagt tcatgtcgtt ggcgtgcaga tcgatatcgt
     2341 tctgtggcgg gatcactacc gtggttttga atttacgcgg caggtaagtc tggccgagga
     2401 tcggttcttc atcagtggtg gcgacttttt cctgatcgag ccagatctcc gcatacgcgc
     2461 gggtacgagg cagcagatgt tcagagattt tcttcgccca ctcgtacgct tctgcgtgca
     2521 gctgcgactc gtaagggttc gaggtgcaga gtacgttacg gttcatgtcg ttagctgtcg
     2581 ccagcgcatc aagaccgacc gagtgcagca tctggtgcac cggtttgacg ttctttttca
     2641 gaatgccgtg gaactgaaac gtctggcggt tggtcaggcg aatgctgcca tagatggtgt
     2701 tttcaccggc aaatttgtcg atcgcctgcc actgtttagt ggtaatcacc ccacccggca
     2761 gacgacagcg caacagcatc gcatggcgcg gctccagctt ctgttcagca cgttcggcgc
     2821 ggatgtcgcg gtcatcctgc tgatacatgc cgtggaagcg gatcagcagg aagttgtcgc
     2881 ccttaaagcc gccggtcaga ccgtcgttta aatcttccgc aatggtgccg cgcaggtagt
     2941 tgctttcaag cttcatgcgc tcggcgtctg tcagttttcc ttcgaccact aaaggccctg
     3001 gatgtttttc gctcattagt agacatctcg ctgataacgg cgctctacgc gcagctcact
     3061 taaaaattca tccgccgctt cggtgtccat gccaccaaat tcggcaatca cttccagaag
     3121 tgcctgctca acgtctttcg ccatgtgatt agcgtcgccg cagacataaa tgtgggcacc
     3181 gtcattgatc cagcgccaca gctccgcgcc ctgttcgcgt agtttgtctt gtacgtaaac
     3241 tttttctttt tgatcgcgcg accaggcgag atcgatacgt gtcagcacgc cctctttgac
     3301 gtagcgctgc cactccacct ggtagaggaa atcttccgta aaatgcgggt tgccaaagaa
     3361 cagccagttt ttacccggtg cttcatcggc agcacgttgc tgcataaagg cgcggaacgg
     3421 cgcgataccg gtgcctgggc caatcataat caccggggtt tccgggttag cgggcaggcg
     3481 gaagttatcg ttatgttcga taaatacgcg gagttcgccc tcttcttcca cgcggtccgc
     3541 gaggaagctg gaggcaccac cggcacgggc gcggccttcc acgtcgtaac gcaccacacc
     3601 aacggtgacg tgtacttcgt tctcgacttc cgcctgcgag gaggcgatgg aatacagacg
     3661 cggcgtcagc gggcgcagca gattaattag cgcttcggca tcaagctgtg ccggggagaa
     3721 acgcaccatg tcaacaatcg gcgtcgtcgc ggcgtaatgc tgtaacttcg ctttatcgcc
     3781 caccagcggc agcagtgttt cactgcgggt aagcgtggcg taattctcaa caatgttggc
     3841 ggtgttgacg gtcagttcga agtgccactg tagcgcttcg ttcagaggca acgttttgcc
     3901 ctcgacggtg acaggttcat cgcctgtcag ccacagcagt tcgacaagtt ctttcaccag
     3961 tgccggatcg ttctgatacc agacgcccag cgcgtcaccc ggctggtaac gcaggcccga
     4021 gtcacctaag tcaatttcga tatggcgaac gtctttttca gagttacgcc cggtaatttt
     4081 ctggttaaca gagaggctag ccaccagtgg cgcgtctttg ctgtacgggc tggtgtggat
     4141 ttcatttacc gcgccagtag cgacggattg cgaaggtgcc gcgacaggcg cacgcgattt
     4201 aagcgcatca accacgcggg cgcgccactc gctggcagca gcctggtatt caacatcggc
     4261 atcgacacgg tcgagcaggc gttcaccgcc cagttccgcc agcttgctgt cgaaatcttt
     4321 cccggactgg cagaaaaatt cataagagct atcgccgagg ctaaacacgg caaacgcggt
     4381 gttttccagc tttggcgctt ttttggagaa caggaactta tgcagcgcga cggcttcttc
     4441 cggcggttcc ccttcccctt gcgttgacgt caccacgata agcagttttt cgctggcgat
     4501 ttgtttgaat ttatagtcgc ccgcgttcac cagcttaacg ttcagttttg ccgtcaataa
     4561 atcatcgcgt aatgcttcag caacccggcg cgcattgccg gtttgcgaag cagagataat
     4621 agttataccc ggcatttctg cggctggcgc tggcgtcgct gcaagcgcag caggctgctg
     4681 attgagcacg ccccagaaat agccagaaac ccaggcaagc tgggtgggag ttaaatcggt
     4741 cgtggccgcc tgaaggcgtg ccagttgctc cgggttcagc ggaagcaacg cggaaggtgg
     4801 gacctgtgtc gtcatgcgtc gttatgttcc agtaagcaaa gctgtttctg cgccctgtca
     4861 gcgcccaaaa acagaagaga aggtaaggtt aacggggcaa atggtgtgga ttaaagacgg
     4921 gatagcgata actaataacc aaatcgacta acctgtttta gcaatagtct ttaacaacaa
     4981 aatagattaa ccaacccaat gaaaaacaaa tgaatttagc caatcattaa gataaatcag
     5041 cgattttgcg caacaaagtc gttttagata atgcgaaaaa acagcctttc cggtactcta
     5101 cggcggtttt atcgtcctgt agagaaatta tgatgtccac cacgttattt aaagatttca
     5161 ccttcgaagc cgctcaccgc ttaccacacg tcccggaagg gcataaatgt ggtcgcctgc
     5221 acgggcattc ctttatggtg cgactggaaa ttaccgggga agtcgatccg catacgggct
     5281 ggattatcga tttcgctgaa ctaaaagcgg cgtttaaacc aacctacgag cgcctcgatc
     5341 accattatct caatgatatt ccaggtctgg aaaacccaac cagcgaggtt ttagcaaaat
     5401 ggatttggga tcaggttaaa cccgttgtgc cgctgttaag tgcggtgatg gtaaaagaaa
     5461 cctgtaccgc aggttgtatc tatcgcggcg aatgataaga gtgtgtcggc ggtcaatttc
     5521 ccttaagtaa cgctatgtta gggtgttgtg ttctggatat ctggggcatg acatggaaga
     5581 cgactgcgac attattatta ttggtgccgg tattgcaggc accgcttgcg cgttacgctg
     5641 cgcgcgagcg ggtttatccg ttttgttact ggaacgcgct gaaatccccg gcagcaaaaa
     5701 tctttccggc gggcggttat atacccatgc actcgcggaa ctcctccctc agtttcatct
     5761 gaccgcgcct cttgaacgac gcatcactca cgaaagcctt tccctgttaa cgcccgatgg
     5821 cgtaacgacg ttttccagct tacagcccgg cggtgaatcc tggagtgtat tacgtgcacg
     5881 gttcgatccg tggctggttg ccgaagccga aaaagaaggt gtcgaatgca tccccggagc
     5941 gacggtggat gcactgtatg aagaaaacgg cagggtgtgt ggtgtcattt gtggtgacga
     6001 tattctccgc gcccgttatg tggtgctggc agaaggtgcc aacagcgtcc tggctgaacg
     6061 tcacggatta gtgactcgtc ctgctggcga agcgatggcg ttggggatca aagaagtgct
     6121 gtcgctggaa acatccgcta ttgaagaacg ttttcatctg gagaaaaacg aaggcgcagc
     6181 gttgctgttc agcggcagga tctgtgatga cttacccggc ggcgcatttc tttatactaa
     6241 tcaacaaacg ctctcgttag ggattgtttg cccgctctct tcccttacgc aaagtcgtgt
     6301 tccggcaagc gagctgctga ctcgctttaa agcgcatccg gcagtgcgcc cgcttatcaa
     6361 aaacacggaa tcactggagt atggtgcgca tctggtgcca gaaggtggct tgcacagtat
     6421 gccggtgcaa tacgccggta acggctggct gctggtgggc gatgcgttgc gcagttgcgt
     6481 caataccgga atttccgtgc gcggcatgga tatggcgctg actggcgcgc aggcggcggc
     6541 acaaacgctg ataagcgcct gccagcaccg cgagccgcaa aatctgtttg cgctttatca
     6601 tcacaacgta gagcgcagcc tgctatggga tgttctacaa cgttatcagc atgttccggc
     6661 gcttttgcaa cgccctggct ggtatcgggc gtggcctgcg ttaatgcagg atatttcccg
     6721 cgatttatgg gatcagggtg ataaacctgt tccaccgctg cgccagttat tctggcgtca
     6781 tttacgtcgt catggcctgt ggcatctggc gggcgatgtt atcaggagtc tgcgatgtct
     6841 gtagcccata atctctggcg cgttgctgat gcgccgcaca ttgttccggc tgactccgtt
     6901 gagcgtcaga tggtacaacg gttgattaac gcctgtccgg caggtctttt ttcgctcaca
     6961 ccggaaggtg acttacgcgt tgattatcac ggctgcctgg agtgtggcac ctgccgtttg
     7021 ctgtgcgacg aatccacact acaacagtgg cgctatccgc cttccggatt cggcatcacc
     7081 taccgctttg gataaataat aaggataatt tatgcccctc ttacacctgc tccgccagaa
     7141 tccggtgatt gctgccgtta aagataatgc cagcctgcaa ctggcaatcg actctgaatg
     7201 ccagtttatt tccgtgttgt acggcaatat ctgcaccatc agtaatatcg ttaaaaagat
     7261 taagaacgcc gggaaatatg cttttattca tgttgatttg ctggaaggcg cgtcaaataa
     7321 agaagtcgtg attcagtttt tgaaactggt gaccgaggcg gacggcatta tcagtaccaa
     7381 agcctcaatg ttgaaagcgg caagagcgga aggttttttc tgtattcatc gcctgtttat
     7441 tgttgattca atttcgtttc acaacattga taagcaagtt gcgcaatcga atccggattg
     7501 tattgagatc ctgccaggct gtatgcccaa agtgctgggc tgggtgacag agaaaatccg
     7561 ccaaccgctg attgccggtg ggctggtgtg cgatgaagaa gatgcgcgta atgcgattaa
     7621 cgcgggtgtc gtggcgcttt ccaccacgaa taccggggtc tggacgttag cgaaaaaatt
     7681 actttgacgg gataatcgta accaattgaa tttggtttga tttgtaggcc gcacgccaca
     7741 tccgacattc agcgcctgat gcgacgcttg acgcgtctta tcaggcctac aagtccccag
     7801 gccagaaccg taggcaggat catccggcaa cacgttattg gcaatctgcg tgaatgtttg
     7861 tcaccagtgc ctccagcacc actttccagt catcaaccac gccaacatct acctgtgaaa
     7921 ataccgcagc gctggcgtca tgattaatcg ccaccacaaa cttgctattg cgtacgcccg
     7981 ccattaacgc tgctgcgcca gaagcgccaa caacaataca cacctcaggt gccagcagat
     8041 gtccggaaat accaatcact ttttcagcat caacgccacc gttcatcact cgcgcccgac
     8101 tgtagcccac ttcggctccc agcttttctg ccagcatagc gatttcctgg ttatctgcct
     8161 ctccgccctg ccccaccacc agcacccgtc tggcctcagc taatgggtcg cgagtaacat
     8221 ttttcagatc ctctgtgcta accagccagt cgggaagcgc acccgggaca atgttcagtt
     8281 gttgcattcc agagggcaac gtggcgtttt tcgctgctcc cgcctgacgc gcctgggaaa
     8341 ggcatagcgg gcgtttttct gtttgcagcg tcgccgttag cgcatttccc cagtgggatt
     8401 tgcgcacgct tacggttgga atatccaacg aagtcacctg gcagatgctt gcaccatgta
     8461 aacgccaggc cagccgggtc gacagttcat cgccaaacgt tcccggcgga aagagaacaa
     8521 cgtccgcagg cgttcgctgc cactgttcca ccaacgcatc cagcacctgt tccgccacta
     8581 caggctgagg ttcaatctgc cagtgcgcca gggtacaacc gctgaaatcc tgcgccgcca
     8641 gccagcttgc tattgcggca ttctcctgat ttattgtcac aattgcgata ttcatggctg
     8701 catcctttgg cgtaaatagt cctgccatag cttttgagct ttttctgcta ccgtttgccc
     8761 atcaatcagc gtcgctccgc gtcgttgctc tgctcttgcc agttgcagac actgcatcgc
     8821 tggcatttcg gcagcaacag ttttacggat aatttctgct ttccccgcag ccattcgctg
     8881 gcgcattccc ggtactggca aggccacttc accgcactga cgcacggcaa tcaccgcagg
     8941 caagcgaacc cggcaacaac acaacccatg ttcagtacgc tgttcgaggg tgataaacag
     9001 cgcgtcgaga gtgaaacgct ccacctgggt gaagcagggc cagcccagca tttccgccag
     9061 caaaaatggc gtttgcccat tctgcccttc gctgctttgg cagccggtaa ttatcagatc
     9121 cagcggattc tgacgctgcc attcggcaat atggcgagcg acaaattccg gcgcaaagcg
     9181 cagatctgcc gccgtctcca gcaatacagc ttcctcaaac ccaagagcca tgagatagcg
     9241 cagccagtgc aacgcccgtt catcccccat gctcaacgcg gttaaagaca tcggcgtacc
     9301 gttttttctc tgcgccaaca gcagcgcggc ggcagcctgt tcatcagcac cgagtaaact
     9361 tcgcagtagc gaaatatccg gtccgctatt accctgagcc gccgcccgcc actctttttc
     9421 cgccagcatt ccggcatccg gttcggcttt aaacgctaac agaatgttca ttcgcacctc
     9481 ctacatttcc cgccgccacc agcgggaggg ctttggtttc tggtgcccat agccaggtca
     9541 caacaaaacc aaccaacaac actgtcgcca ggagcaataa ggtgacttgc attccccact
     9601 gcgccagcac ccacggcagc aggccagtac ttaccgccgc gccaagtcga ctcatggcgg
     9661 tggcaaaacc gacgcccagc gagcgaatgt cagtaggaaa actttccgca ggcaaaatgc
     9721 ccaccagatt actgactgcc gaaatggtgg tgctgaagag aacaaaaagc agcagcgtta
     9781 atgaactgcc ggaaggcaaa caggccatta ctaccagcgt tgccgccagc agcaaaaaac
     9841 ttcccagcaa aaatttgcga tgtgccagca ggtgcgtcag aactaatccc agcagcgcgc
     9901 ccacaattaa caacgcatta agcatcaggc tggcagtcag cgcatcttcc agaccaatag
     9961 tctgggcgat agttggcagc caggtataaa tcacaaacca tgggattacg aggcagacaa
    10021 agaagacgct gttaaacgcc gtgcgccgcc agtaacgcga agagaacaag gttttgatgt
    10081 gtttatgggt cgccgttacc acttcatcgc ccagtaaaac atggggacca aaatagcgat
    10141 gcacgatagc gtgagcttct gcaaaacgcc cctggcgcag tagccagcgt ggcgattctg
    10201 gcgttcccca gcgtaataac gtaatcaaca acgcgggcag agctgccgat gccagtagcc
    10261 agcgccaggc ctccgggttt tcggaaataa agtgatgtcc ggcaatactt gccagcacat
    10321 agcctacggt ccacaccacg ctgaatgcgc ccagcaaaac accgcgatgg cggcgcgggg
    10381 aaaattcagc cagcaaggtg tgaccgactg agtaatcgcc tcccagccca atgccgatca
    10441 aaatgcgcag cccaataaga tgctctggcg tggtggcaaa aaattgcaag aacgaagcga
    10501 gcgtaatcag caaaaagctg aaggtgaaga ttttttgccg accaatatgg tcggagatcc
    10561 acccaagaac caggctgcca agaaacaaac caaggagcgc cgagccgccg atcattcccg
    10621 ccataaacgg cgtcagttgc atggcgggcg taagttgaat aatggcgtaa ccaatgacgc
    10681 cgagaacata accgtcggtc aggtgtgcgc cgaaagtgag cgcagcaatt cgacagtgaa
    10741 aacggttaag cggtaaatca tccattcgca ccggtgaagt gttcatcttt accccttcat
    10801 tgtccggtaa taagaacggc ttctcacctg agaagccgaa aaggcaacgc cccgtcatca
    10861 cgggacgctg cctgacacat tatttttcaa tcggatagat agtacccgcg ttcataatgc
    10921 cgttaggatc gaattgcttt ttcagccctt ccagcaacgc ccacgcgctg ccgtgttcca
    10981 gtttgctcca gtgaacgcga tgtttaccga taccgtggtg gtgcaccatc gaaccaccga
    11041 ggcgaatggt ttcttcgcaa atgattttgt tgagcggatt gtggtacttg tcgatttcct
    11101 cttccggctt acagtcgaca acgttgtagt cgtagacgaa gtacatgttg gtgccgttct
    11161 gatagctatg agaggagtga ccgcccagca tagtgatgtc gtcggcgtgc gggaactcgg
    11221 tacgaatacg gttaataaca ctttcgtaga tttcgtggat gcagctccag cagccggaca
    11281 cttcggtggt aaagcccatg ttgccggttt tgaggatctg cacacgttcg gcagccactt
    11341 tatccggtcc ccagttcagg ttgttaaacc aggtttcgat cagcttgctg tccacgcgct
    11401 ggcattgcgg gtagcgggca acgatttccg caatcccttc gcccgtcgcc ttcgcaatgc
    11461 gagggttacc ttcagccata aagatcagca cgcattttcc gtcggcaaaa tgggtgaagt
    11521 gttgggtgcc atcttcaacg tcatacaaac gagcgatcga cggacgatac ccttccacca
    11581 tgatttcacg caggatgttg aagccggttt tcatgtcttc caggatatag ccgtagaaga
    11641 ggttgttttc cggggtgaat ttaaagattt tcactgttac ttcagtgata tagcacaatg
    11701 caccttcgtt gccgatgatg atgtgacgaa tgtccgggcc agccgcgcgg cgtggcacgt
    11761 ttttaatacg tgtcacggtg ccatcagcca atactgcttc cagaccaacg accatatctt
    11821 cgattgcgcc gtagagtgtg gagaactgcc cgatactgcg ggttgctacc aggccgccca
    11881 tctgcgccag cggctttgac tgcggagaat gccccgtggt gtaacctttt tcacgcaacg
    11941 cgttttccag cacctccagc ggaacgccac attgcgccgt cgcctgcata ttctcaatat
    12001 caatattaat gatttgattc atggcggaac cgtcgagcac caccgagttt tctacaacag
    12061 tttccagccc accttcggtg gcggaagcac cggtacgcgg cacaccgtta attttgtgcg
    12121 cattcataaa attcagcaca cgggatactt gctctgtgga accgagtttt acgaccgctg
    12181 ccggaatcgg caaagtataa atgccatgaa tatccggaaa tttacgaaaa cggtcaatac
    12241 tgttcttctt taatactgtt tcatcggtaa ttacgcgatc tgcaccaaca atttccttta
    12301 gctggtcgac aatcgctgcg cgagataaag acatagtaat tccttcctga taataaaaat
    12361 ggaataatca ttaataagag aaaacagaaa ccaccgttat gggttagcgc actaaataac
    12421 cgccatcaac cactaataaa tgcccgttga cataattcga tgccggactt gcgaggaata
    12481 cggctgcgcc cattaaatcc tgagtatcgc cccaacggtt tgccggaata tgatcaagaa
    12541 cgcgctggtt agtttctgga ttactacggg tcgccagagt gatatcggta gcgtaatatc
    12601 ccggggcaat accattcacc tgaatattat attgacctaa ttcatcgcaa taggatttgg
    12661 tgaatccggc aagagcatgt ttagtggctg aataagcagg tgaccattgt ccacctaagt
    12721 aagagaacaa tgaacagata ttaataattt taccgctttt ctgcgggatc ataatttttg
    12781 ccgcttcgta gcttaactcg aatgcggcgg tcaggttcac atcaatcatt ggatcccagt
    12841 cggcacgacc gaaatccagc accttattca gcttacaaat accggcattg ttaaccagaa
    12901 tatcgacggt accgaagcgc tcacagcagg cagcgataat cttctgcggt gcgccttctg
    12961 cggtgatatc cacctgcatg aagtccactt caacaccctg tttttcaatc atttcctttg
    13021 tttcgccgtt atctttgaca aaacttggaa taaaaatatt tgcgccagct ttggccaacg
    13081 ccattgcaaa tgcctgacct aaaccgctat tcccgccggt aacaattgct gttttacctt
    13141 tcagggagaa aaaatccatt gagaacgcat tgagagattc gattgacata gttagctcca
    13201 atttccgtaa ggcaaaaaaa aagaaaggta acttcccccg cgagagggaa gttacctttc
    13261 tcattatctc tggtaactgg agataatttt tagcataagg acataactgc taatgtgatc
    13321 gtaatcacag tgtgatattc gtattgtcaa ataattttaa aaagcagaag cgtgattact
    13381 ctcacacaac acctctgctt tccatgttat ttattatttc aattactaga gacctgagaa
    13441 aagtaatcca cctgaaaggg aggcttactt ttctcttttt tttgtctatt acaaatggga
    13501 atacgatatg caacacaact catatcgccg ttggataacc ctcgcgataa ttagttttag
    13561 cggcggcgtt agtttcgacc tggcttattt acgttatatt tatcaaattc ccatggcgaa
    13621 atttatggga ttcagcaata ccgagatagg tttaataatg agtacctttg gtattgcggc
    13681 tattattctt tatgccccca gcggcgttat tgccgataaa ttttcacacc gcaaaatgat
    13741 tacttccgcg atgatcatta ccggattact gggtctgtta atggcaacgt atccaccgct
    13801 gtgggtaatg ctctgtattc aggtcgcctt tgcgataaca acgattttaa tgctgtggtc
    13861 ggtgtcgatt aaagccgcct cgttgcttgg cgatcatagc gagcaaggga aaattatggg
    13921 ctggatggaa gggctgcgcg gcgtcggtgt aatgtcgctg gcggtgttta ccatgtgggt
    13981 cttttctcgc tttgcaccgg atgacagcac cagcctgaaa acggtcatta tcatctatag
    14041 tgtggtttac atcttgttgg ggattctgtg ctggtttttt gttagcgata acaacaacct
    14101 gcgcagtgcc aataacgaag aaaaacagtc attccagctt agcgacatcc tggccgtttt
    14161 gcgcatcagc accacctggt attgcagcat ggtgattttt ggtgtcttca ccatctacgc
    14221 cattctgagt tactccacca actatctgac cgaaatgtac ggcatgtcgc tggtggcggc
    14281 gagctacatg gggattgtga tcaacaaaat cttccgcgcg ctgtgcggcc cacttggcgg
    14341 cattatcacc acctacagta aagtgaaatc ccctacccgc gtgatccaaa tcctttccgt
    14401 actcggcctg ctggcgttaa ctgccctgct cgtcacgaac tctaacccgc aatcggtcgc
    14461 gatggggatt ggcctgatta tactgctggg attcacctgt tacgcctcac gcgggctgta
    14521 ctgggcctgc cctggcgaag cgagaacacc gtcttacatt atgggcacca cggtaggtat
    14581 ttgttcggtg attggattcc tgccggatgt cttcgtttac ccaattatcg gccactggca
    14641 agacaccctg cccgcagcag aagcctaccg caatatgtgg ctgatgggaa tggcggcgct
    14701 tgccatggtg attgtcttta cctttttgct gttccaaaaa attcgtactg ctgatagcgc
    14761 ccccgcaata gctagcagca agtaagccag gaaaattaca ggagaaaaat catgtcgaaa
    14821 aaatacatca tagggattga tggcggaagt cagagcacaa aagtggtgat gtacgatctg
    14881 gaaggtaatg tggtttgcga aggtaaaggc ttattgcagc cgatgcacac gccagatgcc
    14941 gataccgcag aacatcctga cgacgattta tgggcatcat tatgttttgc cggtcacgat
    15001 ttgatgagtc agtttgccgg gaataaagaa gatattgtcg gtattggtct gggatccatc
    15061 cgttgctgcc gtgcgttatt gaaagccgat ggcacgccag ctgcgccatt gattagctgg
    15121 caggatgcac gcgtaacgcg tccctacgaa cacactaatc ctgacgtggc atatgtcacc
    15181 tctttttcgg gttatctgac gcatcgctta accggcgagt ttaaagacaa tatcgccaac
    15241 tattttggtc agtggccggt ggattataag agctgggcat ggagtgaaga tgccgcagtc
    15301 atggagaagt ttaatatccc ccgtcatatg ctgtttgatg tgcaaatgcc aggcactgtt
    15361 cttggacaga tcacacctca agccgcgctg gcgacacatt tcccggcagg gctgccggtt
    15421 gtttgtacca ccagtgataa accggtagaa gctctgggag ccggattgct ggatgatgaa
    15481 acggcggtga tttctcttgg cacctacatc gcgctgatga tgaacggtaa agcactgccg
    15541 aaagatccgg tggcgtactg gccgattatg tcttctattc cggaaacatt gctgtatgaa
    15601 gggtacggta tccgtaaagg catgtggacg gtgagctggc tacgcgacat gttaggcgag
    15661 tcgttaattc aggatgccaa agcacaggat ctttcaccgg aagatttgct caacaaaaaa
    15721 gcttcttgtg tgccgcctgg ctgtaatggt ctgatgacgg tgctggactg gctgaccaac
    15781 ccgtgggaac cctacaaacg tgggattatg atcggctttg attccagcat ggattacgca
    15841 tggatatatc gttcgatact ggaaagcgtg gcgctgacgc tgaagaacaa ttacgacaat
    15901 atgtgtaatg aaatgaatca ctttgcaaag catgtgatca ttactggcgg cggttcgaac
    15961 agcgatctat ttatgcaaat ttttgccgac gtgttcaacc ttcctgcacg ccgtaacgcc
    16021 attaacggtt gtgcaagtct gggggcagcg atcaatacgg cggtaggtct ggggctatac
    16081 ccggattacg caacggctgt cgacaaaatg gttcgcgtga aagatatctt tatgcctgtt
    16141 gagagcaatg ccaaacgcta cgacgcgatg aataaaggta ttttcaaagc cctaaccaaa
    16201 catactgatg tgatcctgaa aaaatcgtat gaagtgatgc atggggaatt ggggaatgcg
    16261 gattcgatcc agagctggtc gaatgcgtaa gtgaggaaga ccgggcggga aactgcccgg
    16321 cctgaacata cctgaatggt tatccccgct gacgcgggga acataagttc gacaacttaa
    16381 aaaatgataa caagtccggt ttatccccgc tgatgcaggg aacataataa aaacttcaac
    16441 tagttcggtt tatccctgtt tttacaagga atattaacta cactcgatgt ctttagaata
    16501 aaagacaata cgaatcgcac tttatcaatt ttcacttaaa gtatgaactg g
//
LOCUS       DAPGOF010000106        16371 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.107, whole genome shotgun sequence.
ACCESSION   DAPGOF010000106 DAPGOF010000000
VERSION     DAPGOF010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.107"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..358
                     /locus_tag="RHV13_003035"
     CDS             <1..358
                     /locus_tag="RHV13_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HDV1242549.1"
                     /translation="SEDLHVFFAELATIKDKSDDEWWRALLQSRNDHLRQTALRNAHT
                     PASLLTTLTEPQDRSLAINNPQLAADVKTAWLKEDPSLLLFVEQPDLSQLRDLVKTGA
                     TRKIRSEARHRLEEKQ"
     gene            355..810
                     /locus_tag="RHV13_003036"
     CDS             355..810
                     /locus_tag="RHV13_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242550.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNDGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(888..1799)
                     /gene="rdgC"
                     /locus_tag="RHV13_003037"
     CDS             complement(888..1799)
                     /gene="rdgC"
                     /locus_tag="RHV13_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HDV1242551.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            1924..2832
                     /gene="mak"
                     /locus_tag="RHV13_003038"
     CDS             1924..2832
                     /gene="mak"
                     /locus_tag="RHV13_003038"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HDV1242552.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEEND
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            complement(2975..4159)
                     /gene="araJ"
                     /locus_tag="RHV13_003039"
     CDS             complement(2975..4159)
                     /gene="araJ"
                     /locus_tag="RHV13_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HDV1242553.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(4285..7428)
                     /gene="sbcC"
                     /locus_tag="RHV13_003040"
     CDS             complement(4285..7428)
                     /gene="sbcC"
                     /locus_tag="RHV13_003040"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HDV1242554.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEVYRAFWSQNRAR
                     NQPGGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQNLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVASALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLQVAIQ
                     NVTLEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQARLLTLEKEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLVTRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQRDELPHSEETVVLENWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPGGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            complement(7425..8627)
                     /gene="sbcD"
                     /locus_tag="RHV13_003041"
     CDS             complement(7425..8627)
                     /gene="sbcD"
                     /locus_tag="RHV13_003041"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HDV1242555.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHIRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQTLTESLPVE
                     VLLVRRSREQRERVLASQPRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            8817..9506
                     /gene="phoB"
                     /locus_tag="RHV13_003042"
     CDS             8817..9506
                     /gene="phoB"
                     /locus_tag="RHV13_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HDV1242556.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            9564..10859
                     /gene="phoR"
                     /locus_tag="RHV13_003043"
     CDS             9564..10859
                     /gene="phoR"
                     /locus_tag="RHV13_003043"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HDV1242557.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            11266..12585
                     /gene="brnQ"
                     /locus_tag="RHV13_003044"
     CDS             11266..12585
                     /gene="brnQ"
                     /locus_tag="RHV13_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HDV1242558.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            12661..14034
                     /gene="proY"
                     /locus_tag="RHV13_003045"
     CDS             12661..14034
                     /gene="proY"
                     /locus_tag="RHV13_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HDV1242559.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            14190..16007
                     /gene="malZ"
                     /locus_tag="RHV13_003046"
     CDS             14190..16007
                     /gene="malZ"
                     /locus_tag="RHV13_003046"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HDV1242560.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQNDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDVARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNVAQWQRKEGHGQLTDGILALPAISATVWMN"
     gene            16193..>16371
                     /locus_tag="RHV13_003047"
     CDS             16193..>16371
                     /locus_tag="RHV13_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242561.1"
                     /translation="MTISNSNLLKPFITGAELPPFKEDVFSKTEEQFKKSTHGKSLFI
                     CGNENLVNISAEKID"
BASE COUNT         3648 a         4123 c         4434 g         4166 t
ORIGIN      
        1 cagtgaagat ctccatgtat tttttgcgga actggctacg ataaaagata aatcagatga
       61 tgaatggtgg cgtgcattgc tgcaaagtcg caatgatcat ttgcgccaaa cggcattacg
      121 caacgcacat accccggcgt cgttgttaac gacattgact gagcctcagg atcggtcgct
      181 ggctattaat aatccacagc tggctgccga tgtgaaaacg gcgtggttaa aagaggatcc
      241 atcattactc ttatttgtcg aacaacccga tctttcgcag ttacgtgatt tagtgaaaac
      301 cggggcaacg cggaaaattc gcagtgaagc gcgtcaccgg cttgaggaaa aacaatgaca
      361 caacgtccat ggagcaagtt acagcgggag atctatgact tactgacgcc aacgatcaac
      421 ctgcaaattc actgtacccg ttatcccatg cgcagccaaa atggtggaag caccgattta
      481 ccgcgttatt ggatcacgtt ggataaaaac gtgatctggg attacccgaa agactttatt
      541 gcaggtaacg atggagtgcg taattttcac ggtgaaactt gctggtatcc ttacctgacg
      601 gatatttgtt caatttctga tttattgcgt gaatatattg acaccccaaa agcggagttg
      661 ttaacaaaac aatttaccag cgataaatgg gggttagtga atattctgcg tgctgctgac
      721 aggcgaatag ggatgaggcg attagatcag ttacggcgta aaacgcataa tattgctgcg
      781 ctaaaaatta ttgctcgccg tagcgaataa ttatatgcct ggtgtggctt cgtacgccgg
      841 ataagacgcg gcaggcgtcg catccggcat taaaggaaaa tcagcaatta acgttgtgct
      901 tcgccaccta atccttcaat caggttttga attaacgctg ccagttcacc agtcatcagg
      961 atgaaatcgg catcaaaacg ctgggcgaaa tcttcacggt cgatatcttc gttttgatcg
     1021 cgcagctcgt cgcagaactt aagacgcttg agcgaaccat cgtcgcacat cacaaactga
     1081 atgcgctgct gccagtcgag cgccagttta gtcaccactt ttccggcttc aatgtgattg
     1141 gtgatctctt cgctggtcag atcttgtttc ttcgcgcgga tcacgccgcc atcttccagc
     1201 aacgatttca gctcggcttc atcaagcagc tggaagccct gtgccgcact accggagcga
     1261 acccattcgg tcagcgtcag ttcaatcggg ttttccatgc tcaatggtac aaccggtaac
     1321 gaccccaggc ttttacgcag cagtgccagc gtatcttccg cttttttggc actggcgcag
     1381 tccaccataa tcaaaccgtt aaccgtgtcg atccacatca ttgtctggct aaaacggctg
     1441 aaagcacgcg gcagcagaga gtgcagcact tcgtctttca gcgaatcttt ttcggttttc
     1501 ttcagcttac gcgcctgttc cgcttccagt ttggcgattt tcgcttccag cgcctgttta
     1561 atcaccggag acgggaggat tttttcttct ttgcgtgcgc agataacaat ttgaccattg
     1621 gcaacgtgcg ttaacgcatc gctgtgcgat cccatcggag gaacccagcc catcttcgcc
     1681 atgtcctggc tgccgcatgg ggtaaatgcc atcgaggcta gctgtttttc catctcttct
     1741 gcacgcagcg aaatctcgcg gctaagacgg taaaccatta aatttttgaa ccacagcatg
     1801 ataatttcca cggccttgtc gttaaattta gcgggcatga taacgaattg tcggcggcct
     1861 tgcattgcca atccggttgt ccgtctctac gctattgata ttgaaaaaaa taaggagagt
     1921 accgtgcgta taggtatcga tttaggcggc accaaaactg aagtgattgc actgggcgat
     1981 gcaggggagc agttgtaccg ccatcgtctg cccacgccgc gtgatgatta ccggcagact
     2041 attgaaacga tcgccacgtt ggttgatatg gcggagcagg cgacagggca gcgtggaacg
     2101 gtaggtatgg gcattcctgg ctcaatttcg ccttacaccg gtgtggtgaa gaatgccaat
     2161 tcaacctggc tcaacggtca gccattcgat aaagacttaa gcgcgaggtt gcagcgggaa
     2221 gtgcggctgg caaatgacgc caactgtctg gcggtttcag aagcagtaga tggcgcggca
     2281 gcgggagcgc agacggtatt tgccgtgatt atcggcacgg gatgcggcgc gggcgtggca
     2341 ttcaatgggc gggcgcatat cggcggcaat ggcacggcag gtgagtgggg acacaatccg
     2401 ctaccgtgga tggacgaaga cgaactgcgt tatcgcgagg aagtcccttg ttattgcggt
     2461 aaacaaggtt gtattgaaac ctttatttcg ggcacgggat tcgcgacgga ttatcgtcgt
     2521 ttgagcggac atgcgctgaa aggcagtgaa attatccgcc tggttgaaga aaacgatccg
     2581 gtagcggaac tggcattgcg tcgctacgag ctgcggctgg caaaatcgct ggcacatgtc
     2641 gtgaatattc tcgatccgga tgtgattgtc ctggggggcg ggatgagcaa tgtagaccgt
     2701 ttatatcaaa cggttccgca gttgattaaa caatttgtct tcggcggcga atgtgaaacg
     2761 ccggtgcgta aggcgaagca tggtgattcc agcggcgtac gcggcgctgc gtggttatgg
     2821 ccacaagagt aaaaaaccca gacaattggc acatcatgcc tgatgcgacg cttgccgcgt
     2881 cttatcaggc ctacaaaatg tgccagaacc gcgtagggcg gataaggcgt tcacgccgca
     2941 tccggcaata agcaacgcgc accgtgacag taatctaccc cagtggtttc gccagcaccg
     3001 gactatccgc cgcttgctgg cgcttatagc gaccatacag cagcaacgat gacatcgcag
     3061 caaacgaaag cagggcggca ggcagcgcca cgtaattata tgccagcccc agcgtcagca
     3121 tcatacctcc gcaatatgcg ccgacggcgc taccgaggtt aaacgctatt tgcccacctg
     3181 cggcacctaa taactctccg cctttggcat tttgcagtaa caatatttgc agaggtgctg
     3241 aaagggcaaa taatcccgcg caacaaataa aagcaaaaat aagcgacgtt gttttcatgc
     3301 cgccgcagaa aaagagcatc agcagtgcca gcacaattat aaagtcagtc actgctgcaa
     3361 tgcgcagtgg tgaataacgt cctgaaatcc tgccacttag catatttccc agcaccatcc
     3421 ctagcccaac taacatcata ataaaggtca tcgccgtttc cgaaaaaccg gaaataaaca
     3481 tcatgtatgg ctttacgtag ctgaaccagg caaacacacc tgcgttgcca aacatggtgg
     3541 cggcgaaaat taaccacggg gccgggctgc gcaaaaagtg aaattgttcg cgcagctttc
     3601 ctttcgcctc gtcgcgaata tccggcaccc aaaaatagac cgatgccatc accgcaatat
     3661 taaaaacagc gatcaataaa aaggtgtaac gccagctaaa ttcctgactt aaatacgttc
     3721 ccagcggaat gcccagcaaa ttggcgactg tcatcccgga aaccatcccc gccacggcgg
     3781 cggtgacttt tccgggtttg ataatttttg ataacacgat cgctccgacg ccaaaaaatg
     3841 cgccatgcgg aaagccggaa accaggcgac caatggcaag catcaggtaa gacgaagaga
     3901 gcgtgaacat ggcgttgcca atgacgcaca acgccaccag aaacaacaag atatgtttga
     3961 gtgagtagcg gctggaaaag agtgcgatga ttggcgcacc gaccaccacc cccagcgcat
     4021 aatacgagat catatgcccg gcagcaggaa tcgaaattcc tacgttatgc gccagctccg
     4081 tgagcacgcc cataatgcca aattcggcca tccccaaacc aaacgtgccc agagccaaag
     4141 ataaaatgac ttttttcata ccaccatcac aaccaccaga acggctacca atcaccgtag
     4201 ccaataaagt gatagcttgc aggataacga ccagcaaggc caagagataa ttcgcccctc
     4261 tgtattcatt atcctgctga atagttattt cactgcaaac gtactttcca gtttgctgta
     4321 gcccaggccg ttgatctttt tcactttgat ctgcaccgga atacgctctt tcatcgcttc
     4381 tacgtggcta atcacaccga tggttttgcc actggcgttc agggcatcca gcgcatcaag
     4441 ggcggtatcc agcgtttcgc tatccagcgt gccaaaacct tcatcaagga acagcgagtc
     4501 aatacgtgtt ttatggctga ccagatctga aagcgccagc gccagcgcca gactgacgag
     4561 gaaactttcg ccgccagaaa gggtacgggt gtcgcgtacc gcatctgcct gccaggtatc
     4621 aacaacctcg acttccagcg cctcgctggc tttgcgctgt aacagatagc gcccgtgcag
     4681 ccgggtaagt tgctgattag cgagatggac taaattatcc agcgtcagcc cctgggcaaa
     4741 tttgcggaat ttatcgccct ctttggaacc tattagcgaa ttcagatatc cccagtcctc
     4801 aacctgctgc gtcatttgag caatttgctg cattaaggtt tgttgttgct gacggttatc
     4861 tgcatcctgc ttcagctgct ggcgaatctc gccttgactc gtggtgtttt cacgcagctt
     4921 ttggtgagtt tgcgctaact cttgctgaat ctgctccacc gtcacagtga gagccaaccc
     4981 gccaggtcgg tgttgctgat gctgtgccag cgtttctgct gtctgagtga ccagcgtttg
     5041 cgcctgacgg cgctggtttt ccagattctg cttgagctgt tccagttgcg ttagtgtttg
     5101 ttcatccatt agcgccgcaa ggaacgcctg ctgatcgtca aagacgctgg cctgtagcgc
     5161 ggtgtcaaac tgcgcctggg ctttttgcag actttgcgcc gccagaacgt cctgttgctg
     5221 taacgtctgt tgctggctgt gtagtgcgag acattgttca tgaacctgcc gccagttttc
     5281 cagcaccaca gtttcttcgc tgtgcgggag ttcatctctt tgcggcaacg tttccagaat
     5341 cggcgtcagt tgctgaatac ggttttgcag cgcggttaat tcgttctggc gttgctgcca
     5401 gctctgcgct tcttgctgac gcgtcaccaa ccagctctct tcttcatctt cctgtggcaa
     5461 tgtcagtgca taacccgcca atgccgttaa gagctgctgc tggcgttgtt caatttgctg
     5521 ttgatactgg ataatttgct gattatgcgc ggcaatctgc ccttgtaatt catgtcgttg
     5581 gctgagtaac cgcagctggc gttcgtgctc atcttgtgca tctagccacg gttgaatatc
     5641 gtcctgtggc tgcaaggtga tattgaggct ggccgtgacg gcttgccatt gttgagtaag
     5701 tgcttgctca tcttgtcgga ggctttgcgc ttcgttttca tcgcgctgaa gctgcttcgt
     5761 taatgcatcc agttgcccgc gcagcgccgc accttcttcg ccgagctttt tcacttcttt
     5821 ttccagcgtt aatagccggg cctgattaac gcccggttcc agcgcctgat acgcctcgac
     5881 cgctggatgg ctggtggaac cacaaagtgg acaaggctga cccgcctgta actgcgcgcg
     5941 ctgggcttcc agcgttttga tgcgcgcttc ctgctcgcaa atggttttca catcggcaag
     6001 ttgctgcgtc ttttctttat aacgctggcg catttcgtta agtgcggcat tacgttgcgt
     6061 ctgttccaga gtgacattct ggatagcgac ctgtaactgc gccagacgtt tttgttgggg
     6121 aacaatctgc ccatgcagcg cgaccaggcg ctgacgcagt gggcgttgct cggcatgttg
     6181 cgccagggcg ctagcaactt catcagcggt taacgtcaac gtgatcgccg caagcgcatt
     6241 aagtttttgc tcagcatggg ttaattgttg ctgccattgc cgcagatgct cgcgatcgct
     6301 ggtttgttgg gagaactgcg cacgccaccc cgccagttcg ttgttccact gacggaagcg
     6361 gtcgtgttcc tgtaaccagg tattcaggtt ttgttgctgc tgctgtaatt ctgctgactg
     6421 cttcgccgcg tggtggcgaa tgctcgcgcg aagcgccatt gtgctctgta agcgagtatt
     6481 tacttcttca atctgctggc gagtatgcgc cagcgccgcg ctgtgttctg cgatgcgttc
     6541 ccagtgcgga cgaagatttc gtgccggttg tgccagactg agcgccgcca gttgaggttg
     6601 cgctttttct tcttcggcta acgcctgttg caaggcctgc tgacggcggc tggcttcttg
     6661 ctgcaattcg tccagacgcg ttaaccagtt tagtgattgt tgttcttgct gctgcgcggt
     6721 aagtaactgt ttttcttcgt cagtaagtac ctgcaaactc gctgtcagcg attgcacttg
     6781 ttccggcgtg agcaacgcga cgccgctggc ctgcgcttgc agcttctcca gctcagtgcg
     6841 ggccgatttg tgctgctcaa aaaccatcgc cgagatttgc ccgtagattt cagtgccggt
     6901 taactcctcg agcaattccg cgcgttcttt gggtttggca ttcaggaagg cagcaaattg
     6961 cccctgcgaa agcagcatcg aacgggtgaa gcgcccgtaa tccagtccgg ttaacgtcgc
     7021 cgtcagttcc agcttatctt tcactttgtc ggcgagaatt ttgccatcgg cgcagcgcgc
     7081 cagctctacg cgtggcacct gcaaattacc gccgggttgg ttacgcgccc gattctggct
     7141 ccagaacgcg cgatacactt cacctttcac ttcaaactcc acctccgcca gacattcggc
     7201 ggtatcgcgg gtcatgagat cattttgcga ttgtgaaacg ttagagagac gcggagtttc
     7261 gtgatacagc gccagacaaa tggcgtccag cagggtggtt ttccccgcac cggttgggcc
     7321 ggtaatggca aacagcccgt tgctggcgaa cggctcgcgg gtgaaatcaa tcttccattc
     7381 gccttttaat gagttcaggt ttttcaggcg caggctgaga attttcatgc ttcgtgttct
     7441 ccggcgaggg tatgcaacgt cgtggtgaaa agatgctgca gacgttgctg ctgcgactca
     7501 tccagttctt ccagtgccag acggcgattg aacacctctt cgacgctgag ttcgctgagg
     7561 gtttcacgcg gttggctggc taacacacgc tcgcgctgtt cacgactccg gcgtaccagt
     7621 aatacttcaa caggcaatga ttcggttaat gtctggattt tgcgctgaat atcatgcaga
     7681 tactcatcag tagtgatttc gatatccagc cagacaggcg gctcctgcga tacatcgcgc
     7741 cactgttcca gctgtgcggt aatcgacgcc agatcgcctt tcagcactgc catgggttgc
     7801 gttaccggta cgttcaggtt ttccacgctc tctaatttgc cgtttgaaaa tgtcaccaga
     7861 tggacatatt tactctttcc gcattcatca aaactcagtg gaatggggga gccgcaatag
     7921 cgaatatgtt ccatgccgcc aataatctgt gcgcggtgaa tatgcccgag cgcgatgtag
     7981 tcggctggtg gaaagttttg tgccggaaac gcgtccagcg tgccaatata aatgtcacgc
     8041 acggcgtcac ttttactggc ccccacggtc gttaaatgtc ccgtggcgat gatgggcaga
     8101 ggctgatcgc cgcgcagttt gcaggcatcg gcatagtgtt gttggtaata atcggtaatc
     8161 gctgccagta aatgctgctg tttttcaata ccgttaagcc ccgcctggct ggtaataatg
     8221 tcacgcggac gtaaaaacgg aatggggcac agcactgcgc ctggcgtccc gtcgcgacga
     8281 ggcaagattt gcggcgcatg tccggcgctg gcgaccacgg tagtattgag gaacgccatg
     8341 atatcgcgcg attcattcag cgtggcgacc gagtcatggt ttcctgccag taccaccaga
     8401 tgacagccag tttgctgtaa attgacaaca aaacggttgt ataacgtgcg ggcgtaactg
     8461 ggcggcgagc cggtatcgaa aacatcacca gcaacaataa tcgcatccac ctgatgggtt
     8521 tgtgctgttt ccagcagcca gtcaagaaaa gcctgatgtt cagcttcgcg gcttttactg
     8581 tagaagttct ggccgagatg ccagtctgag gtgtgaagaa tgcgcataac ggttccctgg
     8641 cgaaaaagca tgggcgcgat tatacccaaa cagatgtgcc atttgctttt ttctgcgcca
     8701 cggaaatcaa taacctgaag atatgtgcga cgagcttttc ataaatctgt cataaatctg
     8761 acgcataatg acgtcgcatt aatgatcgca acctatttat tacaacaggg caaatcatgg
     8821 cgagacgtat tctggtcgta gaagatgaag ctccaattcg cgaaatggtc tgcttcgtgc
     8881 tcgaacaaaa tggctttcag ccggtcgaag cggaagatta tgacagtgct gtgaatcaac
     8941 tgaatgaacc ctggccggat ttaattcttc ttgactggat gttacctggc ggctccggta
     9001 tccagttcat caaacacctc aagcgcgagt cgatgacccg ggatattcca gtggtgatgt
     9061 tgaccgccag aggggaagaa gaagatcgcg tgcgcggcct tgaaaccggc gcggatgact
     9121 atatcaccaa gccgttttcg ccgaaggagc tggtggcgcg aatcaaagcg gtaatgcgcc
     9181 gtatttcgcc aatggcggtg gaagaggtga ttgagatgca gggattaagt ctcgacccga
     9241 catctcaccg ggtgatggcg ggcgaagagc cgctggagat ggggccgaca gaatttaaac
     9301 tgctgcactt ttttatgacg catcctgagc gcgtgtacag ccgcgagcag ctgttaaacc
     9361 acgtctgggg aactaacgtg tatgtggaag accgcacggt cgatgtccac attcgtcgcc
     9421 tgcgtaaagc actggagccc ggcgggcatg accgcatggt gcagaccgtg cgcggtacag
     9481 gatatcgttt ttcaacccgc ttttaacgcc ttgctcatcg gacgcagagc agggcttatg
     9541 atttcttaac tggagtatct tacgtgctgg aacggctgtc gtggaaaagg ctggtgctgg
     9601 agctgctact ttgctgcctc ccggctttca tcctgggtgc attttttggt tacctgccct
     9661 ggtttttgct ggcatcggta acaggactgc ttatctggca tttctggaat ttattgcgcc
     9721 tttcatggtg gctgtgggtg gatcgcagta tgaccccgcc accggggcgt ggtagctggg
     9781 aaccgctact atacggctta caccagatgc agctgcgaaa taaaaaacgc cgccgtgaac
     9841 tgggcaatct gattaaacgc tttcgtagcg gcgcggagtc gctgcccgac gcggtggtgc
     9901 tgaccacgga agagggcggt attttctggt gtaacggtct ggcgcaacaa attcttggtt
     9961 tgcgctggcc ggaagataac gggcagaaca tccttaacct actgcgttac ccggagttta
    10021 cgcaatatct gaaaacgcgt gatttttctc gcccgctcaa tctggtgctc aacaccgggc
    10081 ggcatctgga aattcgcgtc atgccttata cccacaaaca gttgctgatg gtggcgcgtg
    10141 atgtcacgca aatgcatcaa ctggaagggg cgcggcgtaa cttttttgcc aacgtgagcc
    10201 atgagttacg tacgccattg accgtgttac agggttacct ggagatgatg gatgagcagc
    10261 cgctggaagg cgcggtacgc gaaaaagcgt tgcacaccat gcgcgagcag acccagcgga
    10321 tggaagggct ggtgaagcag ttgctaacgc tgtcgaaaat tgaagccgcg ccgacgcaat
    10381 tgctcaatga aaaggttgat gtgccgatga tgctgcgcgt tgttgagcgc gaggctcaga
    10441 ctctgagtca gaaaaaacag acatttacct ttgagataga taacggcctc aaagtgtctg
    10501 gcaacgaaga tcagctacgc agtgcgattt cgaacctggt ctataacgcc gtgaatcata
    10561 ctccagaagg cacgcatatc accgtacgct ggcagcgagt gccgcacggt gccgaattta
    10621 gcgttgaaga taacggaccg ggcattgcac cggagcatat tccgcgcctg accgaacgtt
    10681 tttatcgcgt tgataaagcg cgttcccggc aaaccggcgg tagcggatta gggttagcga
    10741 tcgtgaaaca tgcggtgaat catcacgaaa gtcgcctgaa tattgagagt acagtaggaa
    10801 aaggaacacg tttcagcttt gttatcccgg aacgtttaat tgccaaaaac agcgattaat
    10861 ccgcctttgt catcttttat tgccataagc cagccaatgc tggcttattt tctttgcagt
    10921 caaaatacgg gcgttagatt ttacaacggt tggtgatttt ttgttcgcat gattagccat
    10981 gtctttttca cggaaatagt gttttatact ggttggtgat ttcttatcgc tatatacctc
    11041 tggtttttag atccctcctt gctttaaaac gttataagcg tttaaattgc gcttcaggtg
    11101 ctgtcatact gactgcatta acgcggtaaa tcgaaaaact attcttcgcc gcgactggtt
    11161 gggagtattt cccgctaaaa ttgtttaaat ataccgctgt atcatcccca gggattggca
    11221 caaaaattta acgttacaac accacatcca caggcagtat gatttatgac ccatcaatta
    11281 agatcgcgcg atatcatcgc tctgggcttt atgacatttg cgttgttcgt cggcgcaggt
    11341 aacattattt tccctccaat ggtcggctta caggcaggcg aacacgtctg gactgcggca
    11401 ttcggcttcc tcattactgc cgttggtctg ccggtattaa cggtggtggc gctggcgaaa
    11461 gttggcggcg gtgttgacag cctcagcacg ccaatcggta aagtcgctgg cgtactgctg
    11521 gcaacggtgt gttacctggc ggtggggccg cttttcgcta cgccgcgtac agctaccgtt
    11581 tcttttgaag tggggattgc gccgctgacg ggtgattccg cgctgccgct gtttatctac
    11641 agcctggtct atttcgctat cgttattctg gtttcgctct atccgggcaa gctgctggat
    11701 accgtgggca acttccttgc gccgctgaaa attatcgcgc tggtcatcct gtctgttgcc
    11761 gcgattgtct ggccggcggg ttctatcagc acggcgactg aggcttatca aaacgctgcg
    11821 ttttctaacg gcttcgttaa cggctatctg accatggata cgctgggcgc aatggtgttt
    11881 ggtatcgtta ttgttaacgc ggcgcgttct cgtggcgtta ccgaagcgcg tctgctgacc
    11941 cgttataccg tctgggctgg cctgatggcg ggtgttggtc tgactctgct gtacctggcg
    12001 ctgttccgtc tgggttcaga cagcgcgtcg ctggtcgatc agtctgcaaa cggcgctgct
    12061 attctgcatg cttacgttca gcacaccttt ggcggcggcg gtagcttcct gctggcggcg
    12121 ttaatcttca tcgcctgcct ggtaacggca gttggcctga cctgtgcttg tgcagaattc
    12181 tttgcccagt acgtaccgct ctcttatcgt acgctggtgt ttatcctcgg cggcttctcg
    12241 atggtggttt ctaacctcgg cttaagccag ctgatccaga tctccgtacc ggtgctgacc
    12301 gctatttatc cgccgtgtat cgcactggtt gtattaagtt ttacacgctc atggtggcat
    12361 aattcgtccc gcgtgattgc tccgccgatg tttatcagcc tgctttttgg tattctcgac
    12421 gggatcaaag catctgcatt cagcgatatc ttaccgtcct gggcgcagcg tttaccgctg
    12481 gccgaacaag gtctggcgtg gttaatgcca acagtggtga tggtggttct ggccattatc
    12541 tgggatcgcg cggcaggtcg tcaggtgacc tccagcgctc actaaatcac tgaacatttg
    12601 ttttaaccac ggggctgcga tgccccgtgg ttttttattg tgttgatggg ttaggaattg
    12661 atggaaagta agaacaagct aaagcgtggg ctaagtaccc gccacatacg ctttatggca
    12721 ctgggttcag caattggcac cgggttgttt tacggttcgg cagacgccat caaaatggcc
    12781 ggtccgagcg tgttgttggc ctatattatc ggtggtatcg cggcgtatat cattatgcgt
    12841 gcgctggggg aaatgtcggt acataacccg gccgccagct ctttctcgcg ttatgcgcag
    12901 gaaaacctcg gcccgctggc aggttacatt accggctgga cctactgctt tgaaatcctt
    12961 attgtcgcca tcgccgatgt gaccgctttt ggtatctata tgggtgtctg gttcccgacg
    13021 gtgccgcact ggatttgggt actgagcgtg gtgctgatca tttgcgccgt aaacctgatg
    13081 agcgtgaagg tattcggtga gctggaattc tggttctcgt tctttaaagt cgccactatc
    13141 atcatcatga ttgtcgccgg tttcggcatc atcatctggg ggattggcaa cggcgggcaa
    13201 ccgaccggta ttcataacct gtggagcaac ggcggcttct tcagtaacgg ctggcttggg
    13261 atggtaatgt cgttgcaaat ggtgatgttt gcttacggtg ggatcgaaat tatcgggatt
    13321 accgccggtg aagcgaaaga tcctgagaaa tcgataccgc gtgcgattaa ctccgtgccg
    13381 atgcgtattc tggtgttcta cgtcggtacg ctgttcgtca ttatgtctat ctacccgtgg
    13441 aatcaggttg gcactgccgg tagcccgttc gtgctgacgt tccagcatat gggcattacc
    13501 tttgccgcca gcattcttaa ctttgttgtg ctgactgctt cgctgtcggc aattaacagt
    13561 gacgtctttg gcgtaggccg tatgctccac ggtatggcag agcagggcag cgcaccgaaa
    13621 attttcagca aaacgtcgcg tcgcggtatt ccgtgggtta cggtgctggt gatgactacc
    13681 gcgctgctat ttgcggtgta tctgaactac atcatgccgg aaaacgtctt cctggtgatc
    13741 gcttcgctgg caaccttcgc cacggtgtgg gtgtggatta tgatcctgct gtcgcaaatt
    13801 gccttccgtc gccgtttgcc gccagaagaa gttaaggcgc tgaaatttaa agtgccgggt
    13861 ggggtagcaa cgaccatcgg cgggctgatt ttcctgctct ttattatcgg gttgattggt
    13921 tatcacccgg atacgcgtat ctcgctgtat gtcggtttcg cgtggattgt tgtgctgttg
    13981 attggctgga tgtttaagcg ccgccacgat cgtcagctgg ctgaaaacca gtaatccttg
    14041 cgttctgtaa gccggttcag gaggaaatcc tgatccggct attccgaaag ttatccgccc
    14101 ccgtcctcct cccccaaata tccttcagat gatgagtgat cctgcattag gctatggcaa
    14161 ggtgatcaga ttttcatcac aggggaatta tgatgttaaa tgcatggcac ctgccggtgc
    14221 ccccatttgt taaacaaagc aaagatcaac tgctcattac actgtggctg acgggcgaag
    14281 acccaccgca gcgcattatg ctgcgtacag aacacgataa cgaagaaatg tcagtaccga
    14341 tgcataagca gcgcagtcag ccgcagccag gcgtcacggc atggcgggcg gcgatcgatc
    14401 tctccagcgg acaaccccgg cggcgttata gtttcaaatt gctttggcac gatcgccagc
    14461 gttggtttac accgcagggc ttcagccgaa tgccgccagc acgactggag cagtttgccg
    14521 tcgatgtacc ggatatcggg ccacaatggg ctgcggatca gattttttat cagatcttcc
    14581 ctgatcgttt tgcgcgtagt cttcctcgtg aagctgaaca ggatcatgtc tattaccatc
    14641 acgcagccgg acaagagatc atattgcgtg actgggatga gccggtcacg gcgcaggcgg
    14701 gcggatcaac gttctatggc ggcgatctgg acggtatcag cgaaaaactg ccgtatctga
    14761 aaaaacttgg cgtgacggcg ctgtatctca atccggtgtt taaagctccc agcgtacata
    14821 aatacgatac cgaggattat cgccatgtcg atccgcagtt tggcggtgat ggggcgttgc
    14881 tgcgtttgcg acacaatacg cagcagctgg gaatgcggct ggtgctggac ggcgtgttta
    14941 accacagtgg cgattcccat gcctggtttg acaggcataa tcgtggcacg ggtggtgctt
    15001 gtcacaaccc cgaatcgccc tggcgcgact ggtactcgtt tagtgacgat ggcacggcgc
    15061 tcgactggct tggctatgcc agcttgccga aactggatta tcagtcggaa agtctggtga
    15121 atgaaattta tcgcggggaa gacagtattg tccgccactg gctgaaagcg ccgtggaata
    15181 tggacggctg gcggctggat gtggtgcata tgctggggga ggcgggtggg gcgcgcaata
    15241 atatgcaaca cgttgctggg atcaccgaag cggcgaaaga aacacagccg gaagcgtata
    15301 tagttggaga gcattttggc gatgcacggc aatggttaca ggccgatgtg gaagatgccg
    15361 ccatgaacta tcgcggcttt accttcccgt tgtggggatt tctcgccaat accgatatct
    15421 cttacgatcc gcagcaaaat gatgcccaaa cctgtatggc ctggatggat aattaccgcg
    15481 cagggctttc tcatcaacaa caattacgta tgtttaatca gctcgacagc cacgatactg
    15541 cgcgatttaa aacgctgctc ggtagggatg ttgcgcgcct gccgctggcg gtggtctggc
    15601 tgttcacctg gcctggtgta ccgtgcattt attacggtga tgaagtggga ctggatggta
    15661 aaaacgatcc gttttgccgt aaaccgttcc cctggcaggt ggaaaagcag gatacggcgt
    15721 tattcgcgct gtaccagcga atgattgtgc tgcgtaagaa aagtcaggcg ctgcgtcgtg
    15781 gcggctgtca ggtgctgtat gcggaagata acgtggtggt atttgtccgc gtgctaaatc
    15841 agcagcgtgt actggtggca atcaaccgtg gcgaggcctg tgaagtggtg ctacccgcgt
    15901 caccgttgct taatgtcgcg caatggcaac gcaaagaagg ccatgggcaa ctgacagacg
    15961 ggattctggc tttgcctgct atttcggcaa cggtgtggat gaactaaagc atctgtttta
    16021 tttgttacgt cgtagtcacc agccctgaca ctacgcagcg aaaaaagctg ctcaatgtca
    16081 gggctgagtt tgttaaatat ttgtcccgat cgatatgagc gatttttggt attctttgcc
    16141 catgaggtta tgtacaacat gctccaggat aaccctgtag tggagcaaag atatgacaat
    16201 ttcaaattcg aatttattga aaccgtttat tactggtgct gagttgccac cttttaagga
    16261 ggatgtcttt tctaaaacag aagaacaatt taaaaaaagc acccatggca aatcgctttt
    16321 catttgcgga aatgaaaact tagtaaacat ttcagcggaa aaaatcgatg a
//
LOCUS       DAPGOF010000107        15530 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.108, whole genome shotgun sequence.
ACCESSION   DAPGOF010000107 DAPGOF010000000
VERSION     DAPGOF010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.108"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..147)
                     /gene="merE"
                     /locus_tag="RHV13_003048"
     CDS             complement(<1..147)
                     /gene="merE"
                     /locus_tag="RHV13_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum mercury transporter MerE"
                     /protein_id="HDV1242562.1"
                     /translation="MNSPEHLPSETHKPITGYLWGALAVLTCPCHLPILAIVLAGTTA
                     GAFIG"
     gene            complement(144..509)
                     /gene="merD"
                     /locus_tag="RHV13_003049"
     CDS             complement(144..509)
                     /gene="merD"
                     /locus_tag="RHV13_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercury resistance co-regulator MerD"
                     /protein_id="HDV1242563.1"
                     /translation="MSAYTVSRLALDAGVSVHIVRDYLLRGLLRPVAYTTGGYGLFDD
                     TALQRLRFVRAAFEAGIGLDALARLCRALDAADGDGASAQLAVLRQLVERRREALASL
                     EMQLAAMPTEPAQHAESLP"
     gene            complement(527..2212)
                     /gene="merA"
                     /locus_tag="RHV13_003050"
     CDS             complement(527..2212)
                     /gene="merA"
                     /locus_tag="RHV13_003050"
                     /EC_number="1.16.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercury(II) reductase"
                     /protein_id="HDV1242564.1"
                     /translation="MTTLKITGMTCDSCAAHVKEALEKVPGVQSALVSYPKGTAQLAI
                     EAGTSSDALTTAVAGLGYEATLADAPPTDNRAGLLDKMRGWIGAADKPSGNERPLQVV
                     VIGSGGAAMAAALKAVEQGAQVTLIERGTIGGTCVNVGCVPSKIMIRAAHIAHLRRES
                     PFDGGMPPTPPTILRERLLAQQQARVEELRHAKYEGILDGNSAITVLHGEARFKDDQS
                     LIVSLNEGGERVVMFDRCLVATGASPAVPPIPGLKESPYWTSTEALASDTIPERLAVI
                     GSSVVALELAQAFARLGSKVTALARNTLFFREDPAIGEAVTAAFRAEGIEVLEHTQAS
                     QVAHMDGEFVLTTTHGELRADKLLVATGRTPNTRSLALEAAGVAVNAQGAIVIDKGMR
                     TSSPNIYAAGDCTDQPQFVYVAAAAGTRAAINMTGGDAALDLTAMPAVVFTDPQVATV
                     GYSEAEAHHDGIETDSRLLTLDNVPRALANFDTRGFIKLVIEEGSGRLIGVQAVAPEA
                     GELIQTAVLAIRNRMTVQELADQLFPYLTMVEGLKLAAQTFSKDVKQLSCCAG"
     gene            complement(2251..2676)
                     /gene="merC"
                     /locus_tag="RHV13_003051"
     CDS             complement(2251..2676)
                     /gene="merC"
                     /locus_tag="RHV13_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organomercurial transporter MerC"
                     /protein_id="HDV1242565.1"
                     /translation="MGLITRIAGKTGALGSVVSAMGCAACFPAIASFGAAIGLGFLSQ
                     YEGLFIGILLPMFAGIALLANAIAWLNHRQWRRTALGTIGPILVLAAVFLMRAYGWQS
                     GGLLYVGLALMVGVSVWDFISPAHRRCGPDSCELPEQRG"
     gene            complement(2704..2979)
                     /gene="merP"
                     /locus_tag="RHV13_003052"
     CDS             complement(2704..2979)
                     /gene="merP"
                     /locus_tag="RHV13_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercury resistance system periplasmic binding
                     protein MerP"
                     /protein_id="HDV1242566.1"
                     /translation="MKKLFAALALAAVVAPVWAATQTVTLSVPGMTCASCPITVKHAL
                     SKVEGVSKTDVSFDKRQAVVTFDDAKTNVQKLTKATEDAGYPSSLKR"
     gene            complement(2995..3360)
                     /gene="merT"
                     /locus_tag="RHV13_003053"
     CDS             complement(2995..3360)
                     /gene="merT"
                     /locus_tag="RHV13_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric ion transporter MerT"
                     /protein_id="HDV1242567.1"
                     /translation="MSEPQKSEPQNGRGALFAGGLAAILASACCLGPLVLIALGFSGA
                     WIGNLTVLEPYRPIFIGAALVALFFAWRRIYRPAQACKPGEVCAIPQVRATYKLIFWI
                     VAALVLVSLGFPYVMPFFY"
     gene            3432..3887
                     /gene="merR"
                     /locus_tag="RHV13_003054"
     CDS             3432..3887
                     /gene="merR"
                     /locus_tag="RHV13_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hg(II)-responsive transcriptional regulator"
                     /protein_id="HDV1242568.1"
                     /translation="MQINFENLTIGVFAKAAGVNVETIRFYQRKGLLPEPDKPYGSIR
                     RYGEADVTRVRFVKSAQRLGFSLDEIAELLRLEDGTHCEEASGLAEHKLKDVREKMAD
                     LARMEAVLSELVCACHARKGNVSCPLIASLQDGTKLAASARGSHGVTTP"
     gene            3920..4561
                     /locus_tag="RHV13_003055"
                     /pseudo
     CDS             3920..4561
                     /locus_tag="RHV13_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784386.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            complement(5168..5572)
                     /locus_tag="RHV13_003056"
     CDS             complement(5168..5572)
                     /locus_tag="RHV13_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005460020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HDV1242569.1"
                     /translation="MSEALKSLNNIRTLRAQGRELPLEVLEEILEKLNVIVDERRQEE
                     SSKAAELQARQEKLDALRKLMEEDGINPEELLGSFQAKSTATKKSREPRPAKYSFTDE
                     NGETKTWTGQGRTPKALAEQIAAGKSLDDFLI"
     gene            complement(5750..6043)
                     /locus_tag="RHV13_003057"
     CDS             complement(5750..6043)
                     /locus_tag="RHV13_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242570.1"
                     /translation="MNVIAAGIFCFIFYPTAFAYACYKKRWLEWIFLTLIIIFASAIS
                     IQQYNEANFVSELNYPMFISAIYGLSLAWFPFPIAYVVLRFLLDDLQHEYPKQ"
     gene            complement(6069..6305)
                     /locus_tag="RHV13_003058"
     CDS             complement(6069..6305)
                     /locus_tag="RHV13_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242571.1"
                     /translation="MTLSAALQGTFKEMRKDNTVGITVQTCSDIFDSNVPPAYVFWLY
                     RLKAGETLPRGRFFRKRKPIINVMAVVHDAAFEK"
     gene            complement(6346..6801)
                     /locus_tag="RHV13_003059"
     CDS             complement(6346..6801)
                     /locus_tag="RHV13_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000916941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242572.1"
                     /translation="MLKNRFSLLCLAVASSLTLTACDVVDAAYDETLNDNAPEIVIAG
                     GFKIDIQGNAVPVEGYDNCSLNTLQGSVDTDKSNCIVLDRTRKKVPVLVHLSSGPVKE
                     EWKIEHQVEKTKSGKNFIQTRMIRPDGNFVVPASSERGIFSGKHYSQAS"
     gene            complement(6861..7526)
                     /locus_tag="RHV13_003060"
     CDS             complement(6861..7526)
                     /locus_tag="RHV13_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242573.1"
                     /translation="MFSERSVHLITSCTKGKNHQGHVWPTLDIDPKQTPDDAAYAWSN
                     IVDDARSNQAVPALSLYSGNHWSTAKEILNSTRNLELWIISAGMGFLNSRDRVPSYEA
                     TFHQVPFRHDLWWKAITKSLGKHNRCATISQLMQSSPNDEYLIAASPVYISAVQNDIL
                     KGIESLTHPLTQLTIVTSGAYAGPLEEYLIKSSSRMMKELECNMVCLNIKLAQYILKS
                     GSR"
     gene            complement(7584..7964)
                     /locus_tag="RHV13_003061"
     CDS             complement(7584..7964)
                     /locus_tag="RHV13_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001426317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242574.1"
                     /translation="MTSTLDPSHKQIEMWRRATGSEGGLNEYRNWVLENSMRFTAYGI
                     HTVIVEGEAKHITALDDVTLCNEWAKLKRENNRLYAANEKICSGWRGFVLRLLGITLS
                     CRKPVILLGADAKNQNDAVKGADL"
     gene            8607..9425
                     /locus_tag="RHV13_003062"
     CDS             8607..9425
                     /locus_tag="RHV13_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HDV1242575.1"
                     /translation="MTQNQGSEPVPASRTGQLISARDMAMQKFEEGMRLISEASELCG
                     LSLFTSRIMQPNAFGLPSSLDRTIEEGRKEIDRKTWKRLFEETGMDRYWNHKQKEAFN
                     ESLRTDPPVASLEIVKGTLQHALANRRDTLAEGFVDVLNKLDRSFKSNARQYTMPKKL
                     VLRGIFPGVNVLRYNGFSQDNHFCLRDFENIVCICSDTPTPATGGGLSMVDRLTAMRN
                     TEFTGEVSDENGWRCRLFENGNVHICIDSISLLNALNDLISIYFANQLPAAGKK"
     gene            9422..10627
                     /locus_tag="RHV13_003063"
     CDS             9422..10627
                     /locus_tag="RHV13_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HDV1242576.1"
                     /translation="MMIENDKEKSLNDATPPEVQNDIRSESTEKSKEMGRSRYSSIAM
                     IDYFNAIERLCKEKEINPENIDLSFKVHWLRNAVGGSFARSQEMFAEYQKYVKEVPEE
                     ARYLDIPDEVKVALGDIISYITWHYRRSYTAIQNDSVKKAEARSMQLEEEVTQLLQRL
                     EQSATDMNELKLENQALQGRLEIRDSTVKELETRLNVAEAELETCHHQLDSTRHELSL
                     AQQSNDSLSQQLAERKTEIAGHLEYQKKLNEEINTQRSDNAGLSRQCDQLSQTVSDTK
                     AERDRFEQELIAAQNLCTELKSALSGKEGDLVAVNAELTELHKLNESLSADLNKVTLV
                     SQGYEAEVAEQSNELKTLQAKVMKLEATLEAEKTISESLKGTIDTLTGAMAGGGTGKS
                     KQPRSRKTS"
     gene            complement(10907..12226)
                     /locus_tag="RHV13_003064"
     CDS             complement(10907..12226)
                     /locus_tag="RHV13_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1173 family protein"
                     /protein_id="HDV1242577.1"
                     /translation="MSQKFAVMIAYDDDPNVKRYSPDFQTQDEFAKGWQSALKKAHHT
                     SGQKSVITCGCRGKGEKRLYVRALPNGDAFILVKAANTGIEHDPSCVFFSLDARHTGL
                     KGYASGVVRITTEGDMAVRLGIGMTEKDPPEKSEVPPLPHVQRPEGGQASMTLLGLLS
                     LLWTESGLNVWYPKMAGKRNDSLVRYRLLETAKQIRTGRACIGDHLFIGVPDPKQPVA
                     QSQIQRLSSQAMSDKRLMLLSVLPRYDAEKHEKPLKFLPLRNFGGLPLIFFNSEVHWD
                     SVKKRFSSEYAAWKSGAKIVVFALTSPAAVTGRGPSVRAHQIVLMHVSENWIPLDSSY
                     EAVVAEKLDAEHRQYVKPMRYDASISEVFPDFYLLDTKSDKPFPMEVFGMATPAYLAR
                     KQLKKDYYNREYGPYGWWHWDATTASETMVLPHFPESRKPLSTDTPA"
     gene            complement(12249..12416)
                     /locus_tag="RHV13_003065"
     CDS             complement(12249..12416)
                     /locus_tag="RHV13_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242578.1"
                     /translation="MTYFESAEGETVSKERALQELSRHCVPETDFEEFFSDMGVKEQY
                     DAQEVLLWLGY"
     gene            complement(12477..13904)
                     /locus_tag="RHV13_003066"
     CDS             complement(12477..13904)
                     /locus_tag="RHV13_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDV1242579.1"
                     /translation="MKRYTHDLETDLNDVDKTPSLIHKTLLTASTIYDLKYLAQVLND
                     ENGSNWSRASLKRQVTCIPEHCELSIADGRYLQTLIPSRPADYEDRHFSFIDLFAGIG
                     GLRSGFDAIGGKCLFTSEWNTYSSRTYRANWYCDENEHRFNSDIRDITLSNRPEVTDD
                     EAYKFIDASIPDHDVLLAGFPCQPFSIAGVSKKNSMGRKHGFECDTQGTLFFDVARII
                     RAKQPAIFVLENVKNLKSHDKGNTFNIIMKTLDELGYDVANSESTGADDPKVIDGRHF
                     RPQHRERIVLIGFRRDLRLKDGFTLRDIKDFYPDKRPSLSDLLDPSVDSKYILSPKLW
                     EYLYNYAKKHAAKGNGFGFGLVDPSNVNSVTRTLSSRYMKDGSEILIDRGWSHELGET
                     DFHNTYNMDRRPRMLTPRECSRLMGFDKPGESVFRIPVSNTQAYRQFGNSVVVDVFAA
                     VAKLLKSRIEFAASQRLRQFYDEVS"
     gene            14119..14634
                     /locus_tag="RHV13_003067"
     CDS             14119..14634
                     /locus_tag="RHV13_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HDV1242580.1"
                     /translation="MFFITKLLLPIMIVVFPVASWGNSTTFEAKVVKIVDGDTITALD
                     AQNTTIKIRMYGIDAPESKQAFGQKAKQALTTAIATKIVTVIDHGTDIYGRMLGTIWL
                     DGYDINASMVDSGYAWVYRFEDNAIVPGYIKYESAAQKEAKGLWADTNPVPPWQWRQA
                     NEKPRKVKEKK"
     gene            14637..>15530
                     /locus_tag="RHV13_003068"
     CDS             14637..>15530
                     /locus_tag="RHV13_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242581.1"
                     /translation="MMKEPHPLLQLVLNDSGRLTMPVYYRDQQYCPTCLTKVLKSEDG
                     SLAHLGEINQNTCRPSISVVVTKAIIEMLCDGEKIYVNPIRHRGRVLAPSVIFSPDTH
                     TFKPFINTDYQPVGANWKSEKGYKLGLFYLKDRANSINKEEFDFIAVIDPEAMQKEFI
                     SAWSEVDEKNPLETLKQILRSKNNSSLWVKWPGKIEHAQKGNYNENYWFDYQSDNQEV
                     NCTVSVVGIEGNSSGETIYRLQTLLKRKLNEYQLVRSLGTIMLLDNTGNMIPSNHPYF
                     EVISKACINGVRDFVRQNPWVV"
BASE COUNT         4058 a         3760 c         3867 g         3845 t
ORIGIN      
        1 cccgatgaac gcgccggccg tcgtgccggc tagcacaatg gcgagaatcg gcaaatggca
       61 gggacaggtg agcacggcca gcgcgcccca caagtagccg gtgatcggtt tgtgcgtctc
      121 agacggcaag tgctctgggc tgttcatggc agactctccg cgtgctgtgc cggttcggtt
      181 ggcatggcgg ccagttgcat ttcgaggctg gccagggcct cgcgccgacg ctcgacgagt
      241 tgccgcaaca cggcaagctg cgcagacgca ccgtcaccgt ccgcagcatc cagcgcccgg
      301 cacagccgcg ccagtgcgtc caggccgata cccgcttcga aggcagcccg tacaaagcgc
      361 agccgttgca acgcggtgtc atcgaacaag ccgtagccgc ccgtggtgta cgcgaccggc
      421 cgtagcaatc cgcgcagcag gtagtcgcgc acgatatgca cgctcacccc ggcatcaagg
      481 gccagccggg acactgtgta ggcgctcatt gaacacctcc ttttcctcat ccggcgcagc
      541 acgaaagctg cttcacgtcc ttgctgaagg tctgcgccgc gagcttcagc ccttcgacca
      601 tggtcaggta ggggaacaat tggtcggcca gttcctgcac ggtcatacgg ttgcgaatgg
      661 cgagcaccgc cgtctggatc agttcacccg cttccggggc caccgcttgc acgccgatga
      721 gccgtccgct accttcctcg atgaccagct tgatgaagcc gcgtgtgtcg aagttggcaa
      781 gcgcacgcgg cacgttatcc agtgttagca ggcgactgtc ggtctcgatc ccgtcgtgat
      841 gtgcttccgc ctcgctgtag cccacggtgg cgacctgcgg gtcggtgaac accacggccg
      901 gcattgcggt caggtccagg gccgcatcgc cgccagtcat gttgatcgcc gcacgagtgc
      961 cggccgctgc cgccacatag acgaactgcg gctggtcggt gcagtcgccg gccgcgtaga
     1021 tgttcgggct actggtgcgc atgcccttgt cgatgacgat ggccccctgc gcattgacgg
     1081 ctacccccgc cgcttccaat gccaggctgc gcgtgttcgg tgtccggccg gtggcgacca
     1141 gcagcttgtc ggcgcgcaat tcaccgtgcg tggtggtcag cacgaattca ccgtccatat
     1201 gggcgacctg gctggcttgc gtgtgctcca gcacctcgat gccctcggca cggaaagcgg
     1261 ctgtcaccgc ctcgccgatg gccgggtctt cacggaagaa caaggtattg cgcgccaggg
     1321 ccgtgacctt gctgcccagc cgggcaaagg cttgcgccag ctccagcgcc accaccgacg
     1381 agccgattac ggcaaggcgt tcgggaatgg tgtcgctcgc cagggcctcg gtggaagtcc
     1441 agtagggtga ctctttcaag cccggaatcg gcgggaccgc cgggctggca cccgtggcga
     1501 ccaggcagcg gtcgaacatc acgacgcgct cgccaccctc gttcaaacta acgataaggc
     1561 tctggtcgtc cttgaaacgc gcttcaccgt gcagaacggt gatggctgaa ttgccgtcca
     1621 ggatgccttc gtacttggca tgacggagtt cttcgacacg ggcctgctgc tgggccagca
     1681 gccgctcgcg caagatcgtc ggcggtgtgg gtggcatgcc gccgtcgaat gggctttccc
     1741 ggcgcagatg ggcgatgtgg gcggcgcgga tcatgatctt ggacggcaca caaccgacgt
     1801 tgacgcaggt gccgccgatg gtgccgcgct caatcagcgt gacctgcgcg ccttgctcga
     1861 cggccttcag tgctgccgcc atcgcggctc caccgctacc aatgacgacg acctgcaacg
     1921 ggcgttcgtt gccactgggc ttatcagcgg cccctatcca gccgcgcatc ttgtcgagca
     1981 ggccggcgcg gttgtccgtc ggtggcgcat cggcaagcgt tgcctcgtag cccagtccgg
     2041 ccacggcggt agtcagcgca tccgatgacg tgcccgcctc aatggcgagt tgcgctgtgc
     2101 ccttcggata ggacaccagc gccgattgca cgccgggcac tttctccaag gcttccttga
     2161 cgtgagccgc gcacgagtcg caggtcatcc cggtgatttt cagggtggtc atgtattttt
     2221 ccttttctgt ggtggctacg gctgttgccg tcagccacgt tgttctggca attcacagct
     2281 gtccggcccg cagcggcgat gtgctggcga gatgaaatcc cagaccgaca ccccaaccat
     2341 caaggccagg ccgacataga gcagtccacc gctctgccag ccgtaagccc gcattaaaaa
     2401 caccgctgcc agcaccaaga tcgggcctat cgtgccgagc gccgtgcgtc gccactgtcg
     2461 atgattgagc caagcgatag cattggcgag taacgcgatg ccggcgaaca tcggcagcag
     2521 gatgccaatg aatagcccct cgtactggct caagaagccc agtccgatgg ccgcgccaaa
     2581 gctggcgatg gcaggaaaac aggcggcgca gcccatcgcg gaaacgacgc tgccgagcgc
     2641 gccggttttg ccagcgatgc gcgtgatgag tcccatgtgt cgctcccgag ttcggttaac
     2701 ggatcagcgt ttgaggctgg acggatagcc cgcgtcctcg gtcgccttgg tcaacttctg
     2761 gacgttggtc ttggcatcgt cgaaggtgac gacggcctgg cgcttgtcga aacttacgtc
     2821 ggtcttgctc acgccctcaa ccttggaaag cgcgtgcttg acagtgatcg ggcaagaggc
     2881 gcaggtcatg ccaggcacgg acagcgtgac ggtctgggtg gcggcccaca cgggggcaac
     2941 aacggcagcg agggcgaggg cggcaaacag ttttttcatg atgaactcct gtgattaata
     3001 gaaaaatggc atgacgtagg gaaatccgag cgagaccagg accagcgcgg ccacgatcca
     3061 gaaaatgagc ttgtaagtag ctcgcacttg gggaatcgcg cagacctcac ccggtttgca
     3121 ggcttgcgcc gggcggtaga tgcgccgcca ggcgaaaaac agcgcgacca gcgctgcgcc
     3181 gatgaagatc gggcgatagg gttccagcac cgtcaggttg ccgatccatg ccccgctgaa
     3241 ccccaaggcg atcaaaacca gcggccccag gcagcaggcc gacgcaagaa tggcggccag
     3301 cccaccggcg aagagcgcgc cgcgcccgtt ttgtggttca gacttttgtg gttcagacat
     3361 acgcttgtcc tttcaaattt ggtttggata gcttaagctt acttccgtag ttatgtacgg
     3421 agtcaagcga tatgcaaatt aattttgaga atctgaccat tggcgttttt gccaaggcgg
     3481 ccggggtcaa tgtggagacc atccggttct accagcgcaa gggcctgctg ccggagccag
     3541 acaagcccta tggcagcatt cgccgctatg gcgaggcgga tgtaacacga gtgcggttcg
     3601 tgaaatcggc ccagcggctg ggctttagcc tggacgaaat cgccgagcta ctgcggctgg
     3661 aggatggcac ccattgcgag gaagccagcg gcctggccga gcacaagctc aaggatgtgc
     3721 gcgagaagat ggccgacttg gcacgcatgg aggccgtgct gtctgaactg gtgtgcgcct
     3781 gccatgcgcg gaaagggaac gtttcctgcc cgctgattgc gtcactgcaa gacggaacga
     3841 agctcgctgc atcggcgcgg gggagtcacg gggtgactac gccttagcgt gctttatttt
     3901 ccgaattctg agacgacccc tgtgtgttga aagacaagat gagaaccatg atgtgtacgt
     3961 aggtaaattc atccagttac atgtagccgc taataagagc cttaagatcg tcgaaatggg
     4021 cgatgtgaaa ctggtggcca taaccagctc gattcctacc acttttaatg gtgggataaa
     4081 gagatacaaa ggggttacgt atgtgtcact ttcccaaaca gctcaaacca ctatcgactt
     4141 cccgaagcgt atctacaaag agggccagga atgtacttct gtcaccagtc ctgaccaagg
     4201 tcctttcgcc cgggatgtta ggttgaattg ctacgggcga tgtgttgtca ccggcgtaag
     4261 gtccccctgg cgtactgagg ccgcgcactt aacgccacgt catgaagaag gcattcccga
     4321 cgtaacgaac ggaattttac ttcgccgtga tatccataca ctgttcgaca atgaccattg
     4381 cgccataaac cctgacacta tgaaaattta cttcagccgg gaggcccggg agttggatga
     4441 tgatctcctg aaatggcatg ggaatgagat agagacgaca cgcatgcagg ttccggttaa
     4501 catcgaaaac cttcggatac gatggcaaaa atttaaggct aaggatcgtc agcgtaaata
     4561 aagccataag ctgaaccacg gcgaggatta gtcttgttca ctaaatgaca gcgagagatt
     4621 aaatgcaaac ccaaaaagat attacagttg gccagatctg ggaagaagtg gatccaagac
     4681 tgatccggaa agtgcgagtt gttgaggtgg cctcgttaga agggcccaaa ggcatcctaa
     4741 tcgaaaacgt ggagtctggt cgtaagaact gggcgtcgtc atcccgcttt aatggaaagc
     4801 gtggagggta tcgtcttatt tcctgaaggt tttgccaata tgggaaactg aagaggttga
     4861 aaccatcccg gtgagggagc atatctggta aagaagagtc tttaaaaagg ttttacgcga
     4921 cctgggataa taaacgggaa aacgataccc aaaaaaagaa tacagcgatg ataacgataa
     4981 ggaatggtaa gggcttcaat attttgagaa atgacctggc ttcctttgca tctgcatttc
     5041 taatcaaact gtccacatag ggatcacagc tcttaggttc tgaatttttc atatgttctg
     5101 tacctgaaat tgattccgta tgagaaaagg ccaccgaagt ggcctctatc aaaactggtg
     5161 gcaaaagtta gatcaggaaa tcatcaagac ttttaccggc tgcaatttgt tctgccagag
     5221 ctttgggggt acggccctgt ccagtccacg tttttgtttc gccattttca tcagtaaatg
     5281 agtatttggc cgggcgtggt tcacggcttt tcttggtagc cgtagatttg gcctggaatg
     5341 agccaagcag ctcttccggg ttgataccat cttcttccat taacttacgc agggcgtcaa
     5401 gtttctcctg acgagcttgc agttcggcag ctttggatga ttcttcctga cgacgctcat
     5461 caacaatgac attaagcttt tcgaggattt cctcaagtac ttctaaaggc agctcgcggc
     5521 cctgggcgcg aagagtacga atattgttta acgatttgag tgcttcagac atacgacctc
     5581 actttctaat tctttgataa ggatttactt aatcataata cactctataa ggaaaaagat
     5641 gccagcttta tgtcttcagg tacaaagata tcttgctaag aatactcctt atcttccttg
     5701 tgcagactgt ttcgtgcttt cgatgttgtt catgagtctt aaaatctgct cactgtttag
     5761 ggtactcatg ctgtaaatca tcaagaagga agcgcagaac aacataagca atcggaaaag
     5821 gaaaccatgc cagtgacagg ccatatatgg cggagataaa catagggtaa tttaactcac
     5881 ttacaaaatt cgcttcattg tattgttgaa tcgaaatggc agaagcaaaa attataatta
     5941 aagtaagaaa aatccactct agccatctct ttttataaca agcataagcg aaagctgtag
     6001 ggtaaaaaat aaagcaaaaa attccagctg caattacgtt cataaaagta actttcctga
     6061 tagcgcagtt atttttcgaa tgcagcatca tgaacaactg ccataacatt gatgattggc
     6121 tttctttttc tgaagaacct tccgcgcggc aaagtttctc ccgctttgag acgataaagc
     6181 cagaaaacat aagccggagg aacattgctg tcaaaaatat ctgaacaagt ttgaactgtg
     6241 atgcctactg tattgtcttt cctcatctct ttaaaagtgc cttgcaatgc agcggataac
     6301 gtcattttta agaaaccttt ttctgtttga acggatagac ttgacttagc tggcttgact
     6361 gtaatgtttg ccactaaaga tccctcgttc tgaggatgca ggcactacaa agttaccgtc
     6421 aggtctaatc atacgagttt ggatgaaatt tttgccggat ttggtttttt caacctggtg
     6481 ctcgatcttc cattcttctt tgacgggacc ggatgacaaa tgaactagaa ccggtacttt
     6541 tttacgtgtt ctatccagaa caatacaatt tgatttatcc gtatcaacgc ttccctgaag
     6601 agtattcaaa gagcaattat catatccctc aaccggaact gcattacctt ggatatcaat
     6661 tttgaatcct ccagctatta caatttcggg tgcattatca tttaaagtct cgtcataggc
     6721 agcatcaact acatcgcaag cagtaagagt aagtgacgat gccacagcaa gacacaataa
     6781 agaaaatctg tttttgagca tatttgagac cttttataaa aataaaataa caggagaatt
     6841 gtatcattaa cagtctagtt ctaacgacta ccacttttaa gtatgtattg agccagtttt
     6901 atattcaggc acaccatatt gcactctagc tctttcatca tcctagaaga actcttaatt
     6961 aagtattctt caagtgggcc agcatatgcc cctgatgtca cgatcgttag ttgcgtaaga
     7021 ggatgagtca aactctcaat gcccttcagg atgtcattct ggacagcaga aatataaaca
     7081 ggcgaagccg caatcaaata ttcatcattc ggactggact gcattaactg gcttatagtt
     7141 gcgcaacggt tatgctttcc tagtgatttt gtgatggctt tccaccagag atcatgcctg
     7201 aatggtactt gatgaaatgt cgcctcgtaa gagggcaccc gatctcgact atttaaaaac
     7261 cccatcccgg cagagattat ccacaactcc agatttctgg ttgagtttaa aatttccttt
     7321 gccgtagacc agtgattacc tgaatacaag gacaatgccg gtaccgcctg attacttctg
     7381 gcgtcgtcta caatgttact ccaggcatat gctgcgtcgt ccggggtttg ttttggatct
     7441 atgtccaatg tcggccagac gtggccctga tgattcttgc cctttgtaca ggaagtgatt
     7501 aagtgtactg agcgttcaga gaacattatc ttccttacat acccatagaa cagctttcta
     7561 agaacggtgg gctcgcctga aaattacaaa tcagcccctt tcacggcatc attttgattc
     7621 ttcgcgtctg cccccagtaa gatgaccggt tttctgcatg acagtgttat tcctaacaaa
     7681 cgcaacacaa aaccacgcca gccagagcaa attttttcat ttgctgcgta aagcctattg
     7741 ttttctcttt taagcttcgc ccactcgtta cagagggtaa catcatcaag cgctgttata
     7801 tgcttcgcct caccttcgac gataacggta tggataccgt atgctgtaaa tctcatacta
     7861 ttttccagaa cccagttgcg gtattcgttg agtccacctt cggaaccggt agctctgcgc
     7921 cacatttcaa tttgcttgtg tgatgggtca agtgtacttg tcattgaagc ctcgtctcag
     7981 cgataacgct tgaaatagcc cgctttggtc aaaatgaaat tagccatctg actggcgatt
     8041 tgattgtatc ttactcatag tgcccaacct ataggttaaa aaggggcatt ttcgctttat
     8101 agcgtgttca acatcatcaa tcatcccaag aaaggctggc ttattaagcc ggtccatcat
     8161 tttctgtata tctctcatca atttttatat ccggcctgaa gtgcaccgtt caagcacacc
     8221 gatcaggcag ttagtcacta gttaactctt tactagagac taaattccat tgatttacgt
     8281 gacaacgtca cgtcattaag gtttgcaaat gcctaatttt catactatgg gatttaatta
     8341 ggagtaaatc ccataggctt acataaggat gacaattaaa agcgttaaat atcaaccaga
     8401 tagaatgatt acacccaatt aatgggctca tcacattatt tcactggctg gggttattgc
     8461 tggcatatct gaaagtgcta atatgttagt cattagtaat tagtaaagcc tttactaaac
     8521 gcgtttctac gtaaattcta taggttttat agggtcctac ctttgctcaa atcaaaatgt
     8581 tctgacatac caaaatggag tgtaaaatga ctcaaaacca gggttctgag ccagtaccgg
     8641 cgagccggac agggcagctc atcagcgccc gggatatggc catgcagaaa ttcgaagaag
     8701 ggatgcggtt aatctcggaa gcgtcggaac tgtgcggtct ctctctcttc accagcagaa
     8761 ttatgcagcc caacgcattt ggtcttccgt cgtcactgga tcgcaccatc gaggaggggc
     8821 gtaaggaaat tgaccgtaaa acgtggaaaa ggctctttga agaaacaggt atggaccggt
     8881 actggaacca taagcaaaaa gaagccttta acgaatccct tcgtacggat ccgcccgtcg
     8941 cttccctgga gattgttaag ggaacattac agcatgcact ggctaacaga cgagacaccc
     9001 ttgcagaagg tttcgttgat gttctcaaca agcttgaccg aagtttcaaa agcaatgcca
     9061 ggcaatatac aatgccaaaa aagcttgttc ttcgggggat ttttcctggt gtgaacgtgc
     9121 tgaggtacaa cggtttctcg caggacaatc acttttgttt acgcgacttt gagaatattg
     9181 tatgcatctg ttctgatact ccaacacctg caaccggcgg tgggctgagt atggtcgaca
     9241 ggctgacggc catgagaaat acagaattca caggcgaggt cagtgatgag aatggctggc
     9301 ggtgtcgcct gtttgagaat ggaaacgttc atatttgcat cgacagtatc tcgcttctga
     9361 atgctctgaa cgatcttatc agcatatatt ttgcaaacca gcttccggcc gcaggaaaaa
     9421 aatgatgatt gaaaatgata aagaaaaaag cctgaacgat gctaccccgc cagaggtgca
     9481 gaatgacatc cgcagcgaat ccactgaaaa atcaaaggaa atgggtcgtt caaggtactc
     9541 atctatcgcg atgatcgatt acttcaatgc aatcgaacgt ctgtgcaaag agaaagaaat
     9601 taacccggaa aatatcgatc tgagcttcaa agttcactgg ctcagaaacg ctgttggcgg
     9661 ctcgtttgca aggtcacagg agatgttcgc ggagtaccag aagtatgtta aagaggttcc
     9721 tgaagaggcc cgttacctgg atatacccga tgaggtaaag gttgcactcg gcgatattat
     9781 ctcgtacatc acctggcact accgtagaag ctataccgca atacagaatg atagtgttaa
     9841 aaaagctgaa gcacgtagca tgcagctgga ggaggaggta acgcagctgt tacagcggct
     9901 tgagcaaagc gctaccgata tgaatgagct gaaacttgag aatcaggcac tacagggtcg
     9961 attagagatc cgggacagta ccgtcaagga gttagaaaca aggctgaatg ttgccgaagc
    10021 agaactcgaa acctgccatc accagttaga tagcacacgg catgaattga gcctcgcaca
    10081 gcagtcaaat gactcactct cccagcaact ggctgaacgt aaaacagaga tcgctggtca
    10141 ccttgaatat caaaaaaagc tgaacgaaga gatcaacacg cagcgttctg ataacgccgg
    10201 tctttccaga caatgcgacc agttaagtca gactgtgtca gatacaaagg cagagaggga
    10261 caggtttgaa caggaattga ttgctgcaca gaacctttgt actgagctga aaagtgcact
    10321 gtccgggaaa gagggtgacc tggtggcggt gaatgccgag cttactgaac tccataaact
    10381 aaacgagtcg ctgagcgccg acctgaataa agtaactttg gtcagccagg gctatgaagc
    10441 ggaagtggct gaacaaagca atgaactcaa aacgctccag gccaaagtaa tgaagctgga
    10501 agctaccctg gaggcggaga aaacaatctc agaatcccta aaaggcacca ttgatacatt
    10561 aactggtgca atggcagggg gaggtactgg aaagtcaaaa cagccgcgta gcagaaaaac
    10621 atcatgataa atgaagccag gttgatacac ttctctgcat cccctggcac ataagaactt
    10681 ctgcggacat tcagcccgtc cgcaggagtt ttcgtccttc agtcaacaaa tgcactacct
    10741 tttttgagtc tccactttcc agagcagaaa acacgttacc gattctacca acagcttgta
    10801 tctgagtgat aaccccagtc ttgtaatcat ctaggatccc tgacaggaaa gcgttcagtt
    10861 ttgtctgttc gcttagcgaa agcatgtagg acattttttc tccattttaa gcaggtgtgt
    10921 cagttgaaag aggtttgcgt gattctggaa aatgaggcag caccatagtt tcagatgctg
    10981 tggtcgcatc ccagtgccac catccataag gcccatattc acggttgtaa taatcttttt
    11041 tgagttgctt tcgggccaga taagcaggag tggccatacc aaatacttcc atcgggaacg
    11101 gcttatcgct ttttgtgtcg agcaggtaga agtcagggaa cacctcacta atactcgcat
    11161 cataacgcat gggcttaacg tactgccggt gctctgcatc cagcttttct gcaacaaccg
    11221 cctcatagga ggagtccaga ggtatccagt tctcgctaac gtgcatcagc acaatttgat
    11281 gagctcttac agaagggcct ctgccggtta ccgcagctgg tgacgtcaac gcaaagacaa
    11341 ctattttcgc cccggatttc catgctgcgt actccgatga aaatcgtttc tttacgctat
    11401 cccaatggac ttctgaattg aaaaaaatca gcggtagccc cccaaaattc cgcaggggta
    11461 aaaatttaag tggcttctca tgcttttcag catcgtagcg aggtaaaact gacaggagca
    11521 tgagacgttt atcactcatc gcctgtgatg aaagacgctg aatttgcgac tgagcgacag
    11581 gttgtttcgg gtcaggtaca ccaatgaata aatggtcacc tatgcaggct ctgccggtac
    11641 gaatttgttt agcggtttca agcagacgat accgtaccag tgaatcgtta cgtttccctg
    11701 ccattttcgg gtaccagaca ttcagaccag actctgtcca caaaagacta agcaagccca
    11761 ggagggtcat tgaggcctga cctccttctg gtcgctgaac atggggcagg ggaggcactt
    11821 ctgatttttc aggaggatct ttctctgtca taccgatacc gagccttaca gccatatcac
    11881 cttcggttgt aatccggacc acaccactcg catatccttt caggccggta tgccgggcat
    11941 caagggagaa gaatacacag gaaggatcat gctcaatgcc cgtgttagcg gctttgacga
    12001 gaataaaggc atcaccgttc ggtaaagcac gaacataaag tcgcttttct ccttttccac
    12061 gacatccgca ggtgatgact gatttttgac ctgaggtgtg gtgtgccttt ttaagagccg
    12121 actgccaccc tttagcaaac tcatcctgcg tttgaaagtc aggtgaatac cttttgacgt
    12181 ttggatcgtc gtcgtaagca atcatcactg cgaatttttg gctcatagta gtctcctaaa
    12241 caggaagttt aatatcccaa ccagagcaac acttcctgag cgtcatactg ttcctttacg
    12301 cccatgtcgc taaagaattc ttcgaaatct gtttcgggaa cgcaatgcct ggacagttct
    12361 tgtaatgctc gttctttaga taccgtctca ccttcagctg attcgaaata cgtcatggtt
    12421 gacctctgac attattacaa caatcttaag catttaaatt gatgaaggag ctcaggtcag
    12481 ctgacttcgt cgtaaaactg ccgcaagcgc tgggaggctg caaattcaat tcgtgacttc
    12541 agcagtttgg cgacggccgc aaagacatca acgaccacgg agttcccaaa ctgtctgtaa
    12601 gcctgtgtat tggacacagg gatacggaaa acactttccc cgggtttatc gaagcccatt
    12661 agcctgctgc attcacgtgg cgttagcatg cgtggccgac ggtccatgtt gtatgtgtta
    12721 tggaaatcag tttctcccag ttcatgagac cagccccgat caataagaat ttcagatccg
    12781 tctttcatat agcggcttga gagggttctg gtgacactat taacattgga aggatctact
    12841 agtccaaaac caaaaccatt gccttttgcc gcatgtttct ttgcatagtt gtataagtac
    12901 tcccagagtt taggggagag gatgtattta ctatctacgc tcggatccag taaatctgag
    12961 agagaagggc gcttgtccgg ataaaagtct ttaatgtctc tcagagtaaa accgtctttc
    13021 agacgtaggt cgcggcgaaa accaataagg acgatacgtt cacggtgctg gggtcggaaa
    13081 tgacggccat cgatgacttt aggatcatcc gcgcccgtgc tttcgctgtt tgcgacgtcg
    13141 tagccaagct catcgagcgt tttcatgatg atgttaaagg tattaccctt atcgtgactc
    13201 ttcagatttt ttacgttttc cagaacaaaa attgcaggtt gttttgcccg gatgatcctg
    13261 gccacatcaa agaagagcgt accctgagtg tcgcactcga aaccgtgttt gcgacccata
    13321 gagttctttt tactgacacc ggcgatgctg aatggctggc atggaaatcc cgcaagaaga
    13381 acatcatgat ccgggataga ggcatcgata aatttgtatg cctcgtcatc agtaacctcg
    13441 ggccggttgc taagagtgat gtcacggatg tctgaattga agcggtgctc gttctcatcg
    13501 caataccagt tcgcccggta agtacggctg gaataagtat tccattcgct cgtgaaaaga
    13561 catttaccac cgatggcatc aaatccgctt cgcagtccac ctatccctgc aaacagatcg
    13621 ataaagctaa agtgcctgtc ctcataatca gcaggtcgtg aaggtattaa agtctgtaga
    13681 tagcgtccat ctgcgatact cagctcacaa tgctcaggta tgcatgtaac ctgacgttta
    13741 agggatgcac gggaccaatt actgccattt tcatcattta atacctgagc cagatatttc
    13801 aggtcataaa tggtcgaagc tgttaacaaa gttttgtgga tcagagatgg tgttttatcc
    13861 acatcattca ggtctgtctc aagatcatga gtgtaacgtt tcatccaaac tctctcagga
    13921 ataatatctt tgagatagat taacacgctt aatttggatt attttttggt tcaggttcta
    13981 aatttattgt gattattctg cctttaagtt gcaaaaagat tggttttaag cccgcccatg
    14041 aaataatgac ttgttaaaat actgcctgta aataggcttg tggatacatg tctgtactca
    14101 acaaatccag gtgaaatgat gttctttata acaaagttac ttttgcccat catgattgtt
    14161 gtattcccag tggcgtcctg ggggaactca acaacttttg aggcaaaggt ggttaagatt
    14221 gttgacggtg acaccataac tgcgttggat gctcaaaaca caaccatcaa aatacggatg
    14281 tatggtatag acgctcctga atctaaacag gcttttggcc aaaaagcaaa acaggcctta
    14341 accacagcta tagcaactaa aatcgttacc gtgatagacc atggtacgga tatctatggc
    14401 cgtatgttag gtaccatctg gttagatgga tatgatatca atgcctctat ggtggacagt
    14461 ggatatgcgt gggtttatcg gttcgaagat aacgccattg tccctggcta tatcaaatat
    14521 gaatccgcag cacaaaagga ggcaaaaggg ttatgggcag acaccaaccc tgtcccacca
    14581 tggcagtgga gacaggcaaa cgaaaagccc agaaaggtga aagagaagaa ataaccatga
    14641 tgaaagagcc tcatccgtta cttcagttgg tactaaacga ttccggtcgt ctaaccatgc
    14701 ctgtttatta tagagatcaa cagtattgcc cgacttgcct tacaaaggtg ttgaagagtg
    14761 aggatggaag tttggcgcac ctcggggaaa taaatcaaaa tacatgtaga ccttctattt
    14821 cagtagttgt gacaaaagca attattgaaa tgttatgtga cggggaaaaa atatatgtaa
    14881 atccaatacg acaccgaggc cgggtgctgg caccctcagt aattttttct cccgacactc
    14941 acactttcaa accgtttata aatacagact atcagcctgt gggagcaaac tggaaaagtg
    15001 aaaagggata taaactcggt ctgttttatc tcaaagatcg agccaactct atcaataaag
    15061 aggaatttga tttcattgcc gttatagacc ctgaggcaat gcagaaagaa tttatttcgg
    15121 cctggtctga ggttgatgaa aaaaatccat tggaaacgct gaagcagatc ttacgatcta
    15181 aaaacaactc ttctttatgg gtaaaatggc ctggcaaaat tgagcatgcg caaaaaggaa
    15241 attacaacga aaattactgg ttcgattatc aatctgataa tcaggaagtg aactgtacgg
    15301 tctctgttgt aggtatcgaa ggtaacagtt ccggtgagac aatttataga ttgcaaactt
    15361 tgctaaagcg taagctcaat gaatatcaac tggttcggtc tttagggact attatgcttc
    15421 ttgataatac cgggaatatg attccatcaa accatcctta ttttgaagtc atctcaaaag
    15481 catgtataaa tggagtgcgt gactttgttc gtcaaaaccc gtgggtagtc
//
LOCUS       DAPGOF010000108        15381 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.109, whole genome shotgun sequence.
ACCESSION   DAPGOF010000108 DAPGOF010000000
VERSION     DAPGOF010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.109"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(169..333)
                     /locus_tag="RHV13_003069"
     CDS             complement(169..333)
                     /locus_tag="RHV13_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HDV1242582.1"
                     /translation="MEFHENKAKTPFIGLVQLWQEVRRWRRQMQTRRVLQQMSDERLR
                     DIGLRREDVE"
     gene            510..1922
                     /locus_tag="RHV13_003070"
     CDS             510..1922
                     /locus_tag="RHV13_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDV1242583.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLEAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQVARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLM
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQTYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLYRMKIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGDAVYRAME"
     gene            complement(2165..3109)
                     /locus_tag="RHV13_003071"
     CDS             complement(2165..3109)
                     /locus_tag="RHV13_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDV1242584.1"
                     /translation="MTNFTTSTPHDALFKSFLTHPDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRQPLPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKVYTAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIHQRDLMELIDQLAVLLVTGCANDSQITALLNYIL
                     LTGDEARFKAFISELTRRMPHHRERIMTIAERIHNDGWLLGREKGKEEGEQRLLRLLL
                     QNGADPEWIQKITGLSAEQMQALEQPLPESKREPWIEY"
     gene            3576..4808
                     /gene="mdtM"
                     /locus_tag="RHV13_003072"
     CDS             3576..4808
                     /gene="mdtM"
                     /locus_tag="RHV13_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HDV1242585.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            4849..6129
                     /locus_tag="RHV13_003073"
     CDS             4849..6129
                     /locus_tag="RHV13_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HDV1242586.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNNRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKVDINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            6245..7396
                     /locus_tag="RHV13_003074"
     CDS             6245..7396
                     /locus_tag="RHV13_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase subunit D"
                     /protein_id="HDV1242587.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARIRQQWEEGQRLDPRPRLLITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLKMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDFGQLSTRVAAFIEML"
     gene            7406..8173
                     /locus_tag="RHV13_003075"
     CDS             7406..8173
                     /locus_tag="RHV13_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242588.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLGMPVNTHPDAQFAGAIGAAVIGQRVRTRR"
     gene            8170..8427
                     /locus_tag="RHV13_003076"
     CDS             8170..8427
                     /locus_tag="RHV13_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HDV1242589.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGQTESIYCQQDGVWRCIAHYGVSPR"
     gene            8492..9352
                     /locus_tag="RHV13_003077"
     CDS             8492..9352
                     /locus_tag="RHV13_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiK family protein"
                     /protein_id="HDV1242590.1"
                     /translation="MTKSISLSKRISVIVILFAIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDFIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQDSPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDEALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLVGEVIGEISLTKGSRGLSHNIKQAEGIAMDASGNIYIVSEPNR
                     FYRFTPKSSH"
     gene            9420..10598
                     /locus_tag="RHV13_003078"
     CDS             9420..10598
                     /locus_tag="RHV13_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1242591.1"
                     /translation="MPSSSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GAFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLVSALASGLLILAMAWL
                     PPFILVLLIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFALSSQTLWQGAGALSGMMLIALTLLMPSKKHAIAPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAISVLLTLASDSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(10611..11165)
                     /gene="kptA"
                     /locus_tag="RHV13_003079"
     CDS             complement(10611..11165)
                     /gene="kptA"
                     /locus_tag="RHV13_003079"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HDV1242592.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFVEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEW"
     gene            11415..12098
                     /locus_tag="RHV13_003080"
     CDS             11415..12098
                     /locus_tag="RHV13_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242593.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            12095..12556
                     /locus_tag="RHV13_003081"
     CDS             12095..12556
                     /locus_tag="RHV13_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HDV1242594.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            12569..13741
                     /gene="iadA"
                     /locus_tag="RHV13_003082"
     CDS             12569..13741
                     /gene="iadA"
                     /locus_tag="RHV13_003082"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDV1242595.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            13806..14717
                     /gene="hypT"
                     /locus_tag="RHV13_003083"
                     /gene_synonym="qseD"
     CDS             13806..14717
                     /gene="hypT"
                     /locus_tag="RHV13_003083"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HDV1242596.1"
                     /translation="MDDCGAILHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGKLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(14710..15102)
                     /gene="iraD"
                     /locus_tag="RHV13_003084"
     CDS             complement(14710..15102)
                     /gene="iraD"
                     /locus_tag="RHV13_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HDV1242597.1"
                     /translation="MMRQSLQAVLPKISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(15099..15182)
                     /gene="idlP"
                     /locus_tag="RHV13_003085"
     CDS             complement(15099..15182)
                     /gene="idlP"
                     /locus_tag="RHV13_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HDV1242598.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         3465 a         3907 c         3965 g         4044 t
ORIGIN      
        1 caattaatca ctgtgcgttt tttgaccatt atcgacatga gtttaaagag gttagtgaag
       61 aagatattcc tgtacactcc atacctattg atgaatagtc tcgggaaaat ttttataact
      121 taatttaaaa acataaaaac gttttattat ataaatttat atcccccctc actccacatc
      181 ctccctgcgt aacccgatat ccctcaaccg ttcatcactc atctgctgta acactcgtcg
      241 ggtctgcatt tgccgccgcc aacgcctcac ctcctgccag agttgcacta ggccgataaa
      301 cggcgttttg gctttgtttt cgtgaaattc catctgctgc ctcctcatct ttcagaggct
      361 tcatcatccg tcttctggca gttcacaata cagatgcaaa tattagtttt atttaacata
      421 cagacaggtt aaaacagcat ctgcatccgg ttttttagcc tcatctgtac cggtttttct
      481 ctctgtatgg tccaaataag gaatacagca tgacgcgtta tcaacatctg gcgactctac
      541 ttgccgagcg gattgagcaa gggctgtatc gtcacgggga gaaattgccg tcggtgcgca
      601 gcttaagcca ggagcacggc gtcagcatca gcaccgtgca gcaggcgtat cagacgctgg
      661 agacgatgaa gctcatcacc ccacagccgc gttcgggtta ttttgtcgca caacgtaaag
      721 cccagccgcc agtgccacca atgacgcgtc cggtgcagcg tccggtggaa attacccagt
      781 gggatcaggt gctggatatg ctggaagcgc atagcgacag ttccattgtt ccgttaagca
      841 aaagcacgcc ggatgtcgaa acgcccagcc tgaaaccgct gtggcgggag ctaagccggg
      901 tggtgcagca taatctgcaa accgttctcg gttatgactt gttagccggt cagcgggtat
      961 tgcgtgagca ggttgcccgc ctgatgctcg acagcggctc ggtggtcacc gccgatgaca
     1021 tcattatcac cagcggctgc cataactcga tgtcgctggc gttaatggcg gtgtgtaaac
     1081 cgggcgatat tgtcgcggtc gaatccccct gttattacgg ttcgatgcag atgctgcgcg
     1141 gtatgggcgt gaaagtgatt gaaatcccaa ccgatccaga aactggcatc agcgttgaag
     1201 cgctggaact ggcgctggaa cagtggccga ttaaaggcat cattctggtg ccaaactgta
     1261 ataatccgct gggatttatt atgccggacg cgcgcaaacg ggccgttctc tctctcgctc
     1321 agcgtcatga tattgtgatt tttgaagatg atgtctacgg cgaactggca acggagtatc
     1381 cgcgcccgcg gaccattcat tcctgggata tcgacgggcg agtgctgttg tgcagctcgt
     1441 tcagtaaaag cattgcacca ggcctgcgcg tgggttgggt cgcaccgggg cgttatcacg
     1501 ataaactgat gcatatgaaa tacgccatca gcagctttaa tgtgccgtcc acgcaaatgg
     1561 cggcggcaac gtttgtgctg gaaggtcact atcatcgcca tatccggcgg atgcggcaga
     1621 cttatcagcg caatctggcg ctttatacct gctggatacg ggaatatttt ccctgcgaaa
     1681 tctgtattac gcgcccgaaa ggcggatttt tactgtggat cgaattgcct gaacaggtcg
     1741 atatggtctg cgtcgcgcgg cagctgtacc gcatgaaaat ccaggtggca gcaggctcga
     1801 ttttctcggc ttccggcaaa taccgtaatt gtctgcgcat caactgcgct ttgccgctca
     1861 gcgaaaccta tcgcgaagca ctaaagcaaa ttggcgatgc cgtgtatcgg gcaatggaat
     1921 aaattcgaat tagtgccgag gcccctgatt tactctcacg aatcgcctgt aactcgtttt
     1981 atctttgcag ggtcatcgcc acgttacagc atggcttagg caaagtgcgc tttgttcatg
     2041 ccggatgcta tgtgaacgcc ttaccaggtc tacaaaattt ttaaagttca atatatttaa
     2101 tgggttatgt agacctgata agcgtagcac atcaggctgc tttgcgtttg tcatcagcct
     2161 ctgattagta ttcgatccat ggttcgcgct tgctttcagg caagggctgc tctaatgcct
     2221 gcatttgctc tgccgaaagt ccggtaatct tttgtatcca ttcaggatct gccccattct
     2281 gcaacaacaa tcggaggagg cgctgttccc cttcttcttt ccctttctcc cttcccaaca
     2341 gccatccatc attatgaatt cgctctgcaa ttgtcattat tcgctccctg tggtgtggca
     2401 ttcgcctggt aagttcgctg ataaacgcct taaaacgcgc ttcatcgcca gtcagtaaaa
     2461 tgtaatttaa cagcgcggtt atctggctgt cattagcgca ccctgtaacc agtaatactg
     2521 ccagttgatc gataagctcc atcagatcgc gctggtgaat atgcttttgg atcaactcca
     2581 acagagcgac tctgcggtgc tgcacaatct cgtcgtctgg cacgacagtg acatccacca
     2641 gcgggaacgc ggcggtataa actttccgtg cggtagtcgg gtcggcaaat tcgtccagcc
     2701 agcacaggga ccaggggtaa ggactacggc taccgtgata aaacagcatc gggatgacca
     2761 gcggtagcgg ctggcgttta tcatgctcga tatggcgctg catcaccgcc atagaatagc
     2821 gcatcaggcg aaaggccata tgaatatcct cgcggctctg atgttcaatc accacataaa
     2881 tatagccatc gccttcgcgg gtctttaccg accacagaat atcggaatgt agcgcccgca
     2941 atttttcatc gacaaagctg gcggattcaa gttttaagct gtcgagatcg cacagttcac
     3001 gtaaatcttt aggtaagtgg atctccataa aatcccgcgc ggtatccgga tgcgtgagaa
     3061 aagatttaaa caatgcgtca tgcggcgtgc tggtcgtgaa gtttgtcatg gcgatccgtc
     3121 acctgttgat aagatggccc gactttagcg actaacaaaa ccattctcac ccggcaattt
     3181 ctcgttcccc gaggcgcttt ccaggctatt tttgttcgtc tgcaaacgat acatttttcc
     3241 ggaaacaggc tcgcgtttta catttcctgt acccggcaga tattcccctt tccaccgcgc
     3301 tcttttgatt aaccgcaaat tttaattaca cttaaggtgt atattttcta tgcaacccat
     3361 caattcaaga ggtgtaatgt gctgatgact atttgtaatc gttatacatt ctgacctgga
     3421 atcagaaggt atttctctgt ctgtgtgttc acaggcagag tggttgatta catgaaattc
     3481 agtacatttg tccttctcgc ctgcctttcg tcattaccga cggtattgaa tttcgttttc
     3541 cccattgggg ttctccggac aaggagttgt ttgttatgcc acgttttttt gcccgccatg
     3601 ccgccacgct gtttttcccg atggcgttga ttttgtatga ctttgccgcg tacctgtcga
     3661 cggatctgat ccagccgggg atcattaatg tggtccgtga ttttaatgcc gatgtcagtc
     3721 tggccccggc agccgtcagt ctctatctcg ctggcggtat ggcgttgcag tggctgctgg
     3781 ggccactttc cgacaggatt ggccgcaggc cggtgctgat taccggggcg ctaatattta
     3841 cccttgcctg cgctgcgaca atgttcacca cgtctatgac acagtttctt atcgcgcgtg
     3901 caattcaggg caccagtatc tgttttattg ctaccgttgg ttatgtcacg gtgcaggagg
     3961 cgttcggaca gacaaaaggg attaagttga tggcgattat cacctccatc gtactgattg
     4021 cgccgattat tggcccgctt tccggcgcag ctctgatgca ctttgtgcac tggaaagtcc
     4081 tttttgccat cattgcggtt atgggtttta tctcatttgt tggcttactg ctggcgatgc
     4141 cagagacggt gaagcgcggc gcggttccat ttagcgccaa aagcgtcttg cgcgattttc
     4201 gtaatgtctt ttgcaatcgg ctgttcctct ttggcgcagc aaccatctct ttaagctata
     4261 tcccaatgat gagttgggtg gctgtctcgc cggtgatcct gatcgacgcg ggcggcttaa
     4321 caacttcgca gttcgcctgg acacaggttc cggtgttcgg cgcggtgatt gtcgcgaatg
     4381 ccatcgtggc gcgtttcgtt aaagatccga ccgaaccgcg ctttatctgg cgtgccgtac
     4441 ccattcaact ggtcggcctc gcgctgttga ttatcggcaa tctgctgtca ccgcacgtct
     4501 ggctgtggtc ggtgctgggc accagtctgt atgctttcgg gattggtttg atcttcccga
     4561 ccttattccg ctttacgctt ttttccaata acttaccgaa agggaccgtc tccgcatcgc
     4621 tgaatatggt gatcctgatg gtgatgtctg tctcggtcga aatcggccgc tggctgtggt
     4681 ttaacggcgg tcgcttgccg tttcatctgt tagccgtcgt ggcgggcgtt atcgtcgttt
     4741 tcaccctggc aggattgctc aatcgcgtgc gccaacatca ggctgccgag ctggcggagg
     4801 agcggtgatt tttgcgcgat cctgccgtca ggctctattc ttaacgttat gaataaactc
     4861 attgaactca gacgcgccaa aatgttggcg ctctctttac tgcttatcgc cgctgctacc
     4921 tttgtcgtta cgctgttttt gccgcccaat ttttgggtga gcggcgtgaa ggcgattgct
     4981 gaagcggcga tggtcggcgc gctggcggac tggtttgcgg tggtggcgct gtttcgccgc
     5041 gtgccgattc cgatcatttc tcgtcatacg gcgattatcc cgcgtaataa agaccggatt
     5101 ggcgaaaatc tcggtcagtt cgtgcaggaa aaatttctcg atacccagtc gctggtggca
     5161 ttgattcgac gtcacgaacc ggcgttgttg attggcaact ggtttagtca gccggagaac
     5221 gcccgccgcg ttggtcagca tctgttgcag atcatgagcg gttttcttga actgaccgat
     5281 gatgcgcgta ttcagcgcct gcttaagcgc gcggtccatc gggcgattga taaggtcgat
     5341 ctttccggaa ccagtgcgtt gatgctggag agtatgacca aaaacaatcg tcatcaggtg
     5401 ctgctggata cgctgatcgc acagttgatc gcccttctcc agcgcgataa atcgcgcaag
     5461 tttattgccc agcaaattgt tcgctggctg gagagcgagc atccgctgaa agccaaaatt
     5521 ttgcctactg aatggctggg cgaacatagc gcggagttgg tttctgacgc ggtgaattct
     5581 ttgcttgatg atatcagccg cgatcgtgcg catcagatcc gccatgcatt tgatcgcgcc
     5641 acctttgccc tgatcgacaa gttgaaaaac gatcctgaaa tggcagcgcg agccgatgcc
     5701 gtaaaaagtt atctgaaaga agatgaagct tttaaccgct atctcagtga attgtggggg
     5761 gatttacggg aatggctgaa agtggatatc aacagtgaag attctcgtgt gaaagaacgt
     5821 atcgcgcggg cgggtcaatg gtttggcgaa acgttaattg ccgatgatgc cttgcgggcg
     5881 tcgttaaatg gtcatctgga acaagccgcg caccgcgtcg cgcctgagtt ttccgcattc
     5941 ctgacgcgcc atatcagcga cacggtaaaa agctgggatg cgcgggatat gtcgcggcaa
     6001 atcgagttaa atatcggcaa agatctgcag tttatccgtg tcaacggtac gctggtcggc
     6061 ggttgtattg ggctaatttt atatttgctg tcgcagctac cggccttgtt ccccctcggc
     6121 aatttttaga aatccatgaa aaactaagtg gctaaccgcg agagagatca aataaaccac
     6181 actaaaagaa tggtaataat gtggcctctt ttcattatct cccgtggtac ggggaaggaa
     6241 aatcatgtca cttgtcaccg atctacccgc cattttcgat cagttctctg aagctcgcca
     6301 gaaaggcttt ctcaccgtca tggatctcaa ggagcgcggc attccgctgg ttggcaccta
     6361 ctgcaccttt atgccgcaag agatcccgat ggcagccggt gcggttgtgg tttcgctctg
     6421 ttccacctct gatgaaacca ttgaagaagc ggagaaagat ctgccgcgca acctctgccc
     6481 gctgattaaa agtagctacg gcttcggcaa aaccgataaa tgcccctact tctacttttc
     6541 ggatctggtg gtcggtgaaa ccacctgcga cggcaaaaag aaaatgtatg aatacatggc
     6601 ggagtttaag cctgttcatg tgatgcagtt gccgaacagc gttaaggacg atgcctcgcg
     6661 tgcattatgg aaagctgaga tgctgcgctt gcaaaaaacg gtagaagaac gttttgggca
     6721 cgagattagc gaagatgctc tgcgcgatgc tattgcgctg aaaaaccgcg aacgtcgcgc
     6781 actggccaat ttttatcatc ttgggcagtt aaatcctccg gcgcttagcg gcagcgacat
     6841 tctgaaagtg gtttacggcg caacctttcg gttcgataaa gaggcgttga tcaatgaact
     6901 ggacgcgatg accgcccgca ttcgtcagca gtgggaagaa ggccagcgac tggacccgcg
     6961 tccacgcctt ttaatcaccg gctgcccgat tggcggcgca gcagagaaag tggtgcgcgc
     7021 gattgaagag aatggcggct gggtggtcgg ttatgaaaac tgcaccgggg cgaaagcgac
     7081 cgagcaatgc gtggcagaaa cgggcgatgt ctacgacgcg ctggcggata aatatctggc
     7141 gattggctgc tcctgtgttt cgccgaacga tcagcgcctg aaaatgctca gccagatggt
     7201 ggaagaatat caggtcgatg gcgtagttga tgtgattttg caggcgtgcc atacctacgc
     7261 ggtggaatcg ctggcgatta aacgtcatgt gcgccagcag cacaacattc cttatatcgc
     7321 tattgaaaca gactactcca cctcggattt cgggcagctc agtacccgtg tcgcggcctt
     7381 tattgagatg ctgtaaggag tggcagtgac atattcgatt ggcattgatt ctggttctac
     7441 cgccaccaaa gggatcttac tggcagacgg cgtgattacg cgccgtttcc tcgttccaac
     7501 cccctttcgc ccggcaacag caattactga agcctgggaa actctgcgcg aagggttaga
     7561 gacaacgccg tttctgacgc tcaccggcta cggacggcaa ctggtggatt ttgccgataa
     7621 acaagtgacg gaaatctcct gtcacgggct gggcgcacgg tttcttgcgc cagcaacgcg
     7681 cgcggtaatc gacatcggtg gtcaggacag caaagtgatt cagcttgatg atgacggtaa
     7741 cctgtgcgat ttcctgatga atgacaaatg cgcggcgggc accgggcgtt tcctggaggt
     7801 gatctcgcgc acgcttggca ccagcgtcga gcaactcgac agcattaccg aaaatgtcac
     7861 gccgcacgcc atcacgagta tgtgcacagt gtttgctgaa tcagaagtga tcagcctgcg
     7921 ctcagcgggc gtcgcgccag aagcgattct cgccggagtg attaacgcga tggcgcggag
     7981 gagtgccaat ttcattgctc gtctctcctg tgaagcgccg attctgttta ctggtggcgt
     8041 tagtcattgc cagacgtttg cccggatgct ggaatctcac ctgggaatgc cggtaaatac
     8101 ccatcctgat gcgcaatttg ctggcgcaat tggcgcggcg gtaattggtc aacgagtgag
     8161 gacacgccga tgaaagagtt tttattttta tttcactcca cggtcggcgt catacaaacc
     8221 cgcaaggcgt tgcaggcagc gggcatgact tttcgcgtca gcgatattcc ccgtgattta
     8281 cgcggcggct gcgggttatg catttggctg acctgtcccc ccggcgagga aatacaatgg
     8341 gtgatccctg ggcagactga gtcaatttat tgccagcagg atggtgtctg gcgctgcatt
     8401 gcacattacg gggtttctcc gcgttaagga ttcattaagg tcgctacgta tacttctcga
     8461 tatcttattg atttgcctta aagggattac cgtgacaaaa agtatttcgt taagtaaacg
     8521 catctccgtt attgtgattc tttttgccat tgtggcagtg tgtactttct tcgtgcaatc
     8581 ttgcgcccgt aaaagcaatc atgcagcatc atttcaaaat taccacgcca ccattgacgg
     8641 caaagaaatt gcaggcataa cgaataatat ctcttcgctg acctggtcgg cacaaagcaa
     8701 tactcttttt agcactatca acaaacctgc cgccattgtt gaaatgacca ccaatggtga
     8761 ttttatccgc acaattcctc tggattttgt taaggatctc gagacgatcg aatacattgg
     8821 cgataatcag tttgtcatta gcgatgagcg cgattacgcc atttatgtga tttcactgac
     8881 cccaaactcg gaagtaaaaa tcctcaaaaa aataaaaatc ccgctgcaag actccccaac
     8941 caattgcggt tttgaagggt tggcttattc tcgccaggat cacactttct ggttttttaa
     9001 agagaaaaat ccgattgagg tctacaaagt taacgggctg ttaagtagca atgaattaca
     9061 catcagcaaa gacgaggcac tacaacggca atttacttta gatgatgtgt ccggcgcaga
     9121 atttaaccag caaaaaaata cgcttctggt gctgtctcat gaatctcgcg cactccagga
     9181 agtaaccctg gttggcgagg ttatcggtga aatatcacta accaaaggta gtcgaggttt
     9241 gtcgcataac atcaaacagg ccgaagggat tgcgatggat gcctctggta atatttatat
     9301 cgttagcgaa ccaaatcgtt tttatcgctt tacccctaaa tcatcgcatt aacaggctgg
     9361 catatttcac ctttttgtta ttaacttgtt aacaacaaaa atgacataca aggttaaaca
     9421 tgccttcgtc ctcgcatccc gtagaacgct tttctttcag caccgcgctt ttcgggatgc
     9481 tggttctgac cttaggtatg ggattaggtc gcttccttta tacgcctatg ttgcccgtca
     9541 tgatggcgga aggggcgttt tcatttagcc agctctcgtg gattgccagc ggcaactatg
     9601 cggggtatct ggctggcagt ctgctatttt cgtttggcgc atttcaccag ccgtcgcgcc
     9661 tgcgcccatt tctgttggtt tctgccctgg cgagcgggtt gttgatcctc gcaatggcat
     9721 ggttgccgcc atttattctg gtgttattga ttcgcttcct ggcgggtgtc gccagcgccg
     9781 gtatgctgat ttttggttcg acgctgatta tgcagcacac gcgccatcct tttgtgctgg
     9841 cagctttgtt ttctggcgtt ggcattggca ttgcactggg caacgaatat gttctggcag
     9901 gcctgcattt tgccttatcc tcgcaaacgt tatggcaagg cgccggggcg ctttctggca
     9961 tgatgctgat tgcacttacg cttttaatgc cctcgaaaaa acacgccatc gcaccaatgc
    10021 cattggcaaa aacggagcaa cagataatga gctggtggtt actggctatt ctgtacggcc
    10081 tggcgggatt tggttatatc atcgtcgcga cttacctgcc gctcatggcg aaagatgcag
    10141 gctcaccatt gttaaccgcc catctctgga cgttagtcgg cttatcaatt gtgccaggct
    10201 gctttggctg gctatgggca gcaaaacgtt ggggggcttt gccttgcctg acggcgaatt
    10261 tgctggtgca ggctatcagt gtgctgctta ctctcgccag cgactctcct ctcttgctta
    10321 tcattagtag tatcggtttt ggcggtacct ttatgggcac gacatctctg gtgatgacta
    10381 tcgcccgcca actgagcgtt ccgggaaatc tcaatctttt aggctttgtg acgctcattt
    10441 acggcattgg acaaatcctc ggcccggcgc tgaccagtat gctcggcaat ggaacatcgg
    10501 cgcttgccgg cgccactctc tgcggcgcgg cggcgttgtt tattgctgcg cttatcagca
    10561 cagtacaatt atttaagctg caagtggtca cttcatgagg agtgtttccc ttaccactca
    10621 agaaattcga ccgcaaccgt tgatgtcagc cagactccgt tttccgcttg ccagaaaggg
    10681 atacctcgtt tcgccatttc ctgtgctttg acggttaaga taaccggcga tccgtgtctt
    10741 gctcccactt tgcgtgccgt tgcttcatca gcagagagat gcacgtaatg acgatcccct
    10801 gcaatcagcc cctgtttttt tatctcatca aggaaacggc ttgccgtacc gtggtagaga
    10861 aactgtggcg gtgttttttc gacaaaggaa atagcgacct gcgaagtcga atgcccctga
    10921 accgcgcgga tgcacctacc atcactggaa taactaaaac gttttttatc actggtagcg
    10981 accacggtat ccagcaatgc acgggttaac cgcttgcccg ctttttgggc acagagtata
    11041 agcttatcga tatccgccca tccttcacgg tcaagtacga taccaatcgc ctccggtttg
    11101 tgtcggagga caaaacttaa aaacttgctc gtatccgcca gctctttttc gttgtacttt
    11161 gccatctcgt tttcgtccct ggattttctg tttttattta ctgatgtcat tgtcactggg
    11221 tttatgaatt ttctgcaact caatcctgcg cagagccttt tccacacagc gtaatttttc
    11281 cgctatgcaa aaaacgcata gccagaaaca tacttgcatt ttccccatac ccacatggct
    11341 gataaggttt ttccgctcca ggaattttcc tggctcaagc aacacacaaa cacaaaaaaa
    11401 caatggggat tgttatgaca caacaagggg atgctgtagc cggggagctg gcgacagaaa
    11461 aagtgggcat taaagggtat ctggcttttt ttctcaccat aatctttttt tccggtgttt
    11521 tttccgggac agacagttgg tggcgcgttt ttgatttttc cgttctgaac ggatcgtttg
    11581 ggcaactgcc tggtgcgaat ggagccacca cctcatttcg cggggcgggt ggcgcggggg
    11641 ctaaagatgg ctttctcttt gcgctggagc tggcaccgtc agtcattctc tctctgggga
    11701 ttatttcaat taccgatggt cttggtggat tacgcgccgc gcagcagtta atgacacctg
    11761 tgctaaaacc gttgttgggc attccaggga tctgctcgct ggcgctgatc gctaacctgc
    11821 aaaacacgga tgccgccgcc ggtatgacca aagagctggc gcaggaaggt gaaattaccg
    11881 aacgcgataa agtgattttc gccgcatatc aaaccagcgg tagcgccatc atcaccaact
    11941 acttctcttc cggcgtcgcg gtgtttgcct ttctgggcac gtccgtaatc gtacctttag
    12001 ccgtcatcct ggtgtttaaa tttgtcggag ccaacatttt gcgcgtctgg cttaacttcg
    12061 aagaacgtcg caatccaacg caaggagcgc aagcatgaca actcaggtcc gcaaaaatgt
    12121 catggatatg tttatcgatg gtgcgcgccg tggctttact atcgccacga ccaacctgct
    12181 gccaaacgtg gtgatggcgt ttgtcatcat tcaggcgctg aaaattaccg gcctgctcga
    12241 ctgggtgggg catatctgtg aacccgtaat ggcgctgtgg ggattacccg gtgaagcggc
    12301 aaccgtcctt ttagccgcat taatgagtat gggcggcgca gtcggtgttg ccgccagtct
    12361 ggcgaccgcc ggcgcattaa ccggtcacga tgtcaccgtt ttactgcccg cgatgtattt
    12421 aatgggcaac ccggtgcaaa atgtcggtcg ctgcctgggg acagccgaag tgaatgccaa
    12481 atattacccg catatcatta cggtctgcgt gattaacgca ttgctgtcga tctgggttat
    12541 gcagcttatt gtttaacaag gagttaccat gattgattat accgcagccg gttttaccct
    12601 gctgcaggga gcgcatttgt atgcgccgga agatcgggga atttgcgatg tcctcgtcgc
    12661 taacggcaaa attatcgccg ttgccagcaa tatcccttct gacattgtac cgaactgcac
    12721 ggttgtcgat ctcagtgggc agatcctctg cccaggtttt attgatcaac acgtccattt
    12781 gattggcggt ggcggcgaag caggtcccac gacgcgcacg ccggaagtgg cgctaagtcg
    12841 cctgacggaa gcgggcgtca cgtcagtggt tggtctgctg ggcaccgact ctatctctcg
    12901 ccacccggaa tccctgctcg ccaagacccg tgcgctcaat gaagaaggca tcagcgcctg
    12961 gatgctgacc ggcgcttatc atgtcccttc ccgcaccatt acgggttccg tggaaaaaga
    13021 cgtggcgatt atcgatcgtg tgattggcgt gaaatgcgcc atctctgatc accgttctgc
    13081 cgcaccggac gtttatcacc tggccaatat ggcggcagaa tcccgcgttg gcggtttgct
    13141 cggcggtaaa cctggcgtca ccgtgttcca catgggcgac agtaaaaagg cgttacagcc
    13201 tatttatgac ctgctggaaa actgcgatgt gccgatcagc aagctgctgc cgacccacgt
    13261 taaccgcaac gtaccgttgt ttgagcaggc gctggagttc gcgcgcaaag gcggcaccat
    13321 cgatatcacc agcagcattg acgaaccggt cgcccctgcc gaaggtattg cccgcgccgt
    13381 tcaggcgggt attccgctgg cacgcgtcac cctcagctcc gacggcaacg gtagccagcc
    13441 gttcttcgat gacgaaggga atttaaccca tatcggtgtt gcaggttttg aaacgttgct
    13501 ggaaaccgtg caggtgctgg tcaaagacta tgatttcagt atcagcgatg ccctgcgccc
    13561 gctcaccagt agcgtagccg gtttccttaa cctgaccggg aaaggcgaaa ttctgccagg
    13621 caatgatgct gacttgctgg tcatgacgcc agaactgcgc attgagcagg tatacgctcg
    13681 cggcaaactg atggtcaaag acggcaaagc ctgcgtgaaa ggaacgtttg aaacggctta
    13741 aacggttgtc agccgcccga atggacgtag aatgcccgca agggcggctg acagagtaaa
    13801 acgtaatgga tgactgtggt gcgattttgc ataatattga aaccaaatgg ctttatgatt
    13861 ttctgaccct ggaaaaatgc cgcaattttt cccaggcggc agtcagtcgc aacgtctcgc
    13921 aaccggcatt cagccgccgc atccgtgcgc tggaacaggc gattggtgtt gaattgttta
    13981 accgccaggt aacgccgctg caactctcag aacaagggaa aatcttccat tcgcagatcc
    14041 gccatctgtt gcaacagtta gagagcaatc tggcagagct gcgtggcggc agcgattacg
    14101 cgcaacgtaa aatcaagata gccgctgcac actctctttc cctcggtctg ttaccgtcca
    14161 ttatcagcca gatgccgccg ctctttacct gggcaattga agccattgat gtcgatgaag
    14221 cggtcgataa actgcgtgaa gggcaaagtg actgtatttt ttcctttcac gacgaagatt
    14281 tgctggaagc gccgtttgac cacattcgct tatttgaatc tcaattgttc cctgtctgcg
    14341 ccagtgacga acacggagaa gcacttttta acctcgcgca gccacacttt ccgttactga
    14401 attacagccg caactcctac atggggcgat tgattaatcg caccctgacg cgccacagtg
    14461 agttaagttt cagcaccttt tttgtctctt cgatgagcga gcttttaaag caggttgccc
    14521 tcgacggctg tgggattgcc tggctgccgg agtacgccat ccaacaagaa attcgcagcg
    14581 ggaagctcgt tgtactgaat cgggacgaac tggtgatccc gattcaggcc tatgcatacc
    14641 ggatgaatac ccgcatgaat cccgttgccg aacgcttctg gcgtgaactg cgcgagctgg
    14701 agattgtgct tagctgacat tctccagcgt cgcactgcgc caaccgccat tatcccagac
    14761 cagatcaaac gttagcggtt catcgtcgtg gtacagcatt atctcaaggc tgatcgccag
    14821 agtgtggcag tacgcttctt tcagattaac ctgcaacgcc gcaatccgtg gctcatgaaa
    14881 taacagtgca gaacgcagct gacggcaata ccagctctgg agatgtttgt cgctcgggtt
    14941 atgcacttgc cactcgggca tgcctgaaac aagtaatgac ggtaatgccg aatgaggaga
    15001 attaaaaaga gtgaggagat cgctgcatac cgatttacgc agcaatgacg ttttattgcc
    15061 tgaaatttta ggtaaaacag cctgaagtga ttggcgcatc attttgcgca ctcctgacgt
    15121 ttagcaacat atgcggcttg ccctgaacac cgggcaccac tgtattgatg ctcattttcc
    15181 atagccattc ccttttgttc attacatcca tgtcagtctg agacaggcct gaatcctgtc
    15241 agcaaagtat aaaaccttac ttgcctatga atatctactg acttttcagg aaaaaagtca
    15301 tgatttgata aaaatcatca aactcaaaaa aaaaccgcta gtatattctg aatattccga
    15361 atagttacgt agatgaaaat a
//
LOCUS       DAPGOF010000109        15227 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.110, whole genome shotgun sequence.
ACCESSION   DAPGOF010000109 DAPGOF010000000
VERSION     DAPGOF010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.110"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            129..1187
                     /gene="citC"
                     /locus_tag="RHV13_003086"
     CDS             129..1187
                     /gene="citC"
                     /locus_tag="RHV13_003086"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HDV1242599.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTKG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1202..1498
                     /gene="citD"
                     /locus_tag="RHV13_003087"
     CDS             1202..1498
                     /gene="citD"
                     /locus_tag="RHV13_003087"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HDV1242600.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1495..2403
                     /gene="citE"
                     /locus_tag="RHV13_003088"
     CDS             1495..2403
                     /gene="citE"
                     /locus_tag="RHV13_003088"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HDV1242601.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYHDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2414..3946
                     /gene="citF"
                     /locus_tag="RHV13_003089"
     CDS             2414..3946
                     /gene="citF"
                     /locus_tag="RHV13_003089"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HDV1242602.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYHAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3950..4501
                     /gene="citX"
                     /locus_tag="RHV13_003090"
     CDS             3950..4501
                     /gene="citX"
                     /locus_tag="RHV13_003090"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HDV1242603.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALCALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4476..5354
                     /gene="citG"
                     /locus_tag="RHV13_003091"
     CDS             4476..5354
                     /gene="citG"
                     /locus_tag="RHV13_003091"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HDV1242604.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5405..6868
                     /gene="citT"
                     /locus_tag="RHV13_003092"
     CDS             5405..6868
                     /gene="citT"
                     /locus_tag="RHV13_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HDV1242605.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            6982..7788
                     /gene="rna"
                     /locus_tag="RHV13_003093"
     CDS             6982..7788
                     /gene="rna"
                     /locus_tag="RHV13_003093"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HDV1242606.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8017..8427
                     /gene="rnk"
                     /locus_tag="RHV13_003094"
     CDS             8017..8427
                     /gene="rnk"
                     /locus_tag="RHV13_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HDV1242607.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8658..9896)
                     /locus_tag="RHV13_003095"
     CDS             complement(8658..9896)
                     /locus_tag="RHV13_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HDV1242608.1"
                     /translation="MKALTYHGPHHVQVENVPDPGVEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYSAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10117..10545
                     /gene="uspG"
                     /locus_tag="RHV13_003096"
     CDS             10117..10545
                     /gene="uspG"
                     /locus_tag="RHV13_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HDV1242609.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10666..12231)
                     /gene="ahpF"
                     /locus_tag="RHV13_003097"
     CDS             complement(10666..12231)
                     /gene="ahpF"
                     /locus_tag="RHV13_003097"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HDV1242610.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12476..13039)
                     /gene="ahpC"
                     /locus_tag="RHV13_003098"
     CDS             complement(12476..13039)
                     /gene="ahpC"
                     /locus_tag="RHV13_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HDV1242611.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13411..14157
                     /gene="dsbG"
                     /locus_tag="RHV13_003099"
     CDS             13411..14157
                     /gene="dsbG"
                     /locus_tag="RHV13_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HDV1242612.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILATKDPAKTWQEYEASGGKLKLNVSANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14367..>15227
                     /locus_tag="RHV13_003100"
     CDS             14367..>15227
                     /locus_tag="RHV13_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV1242613.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLRGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNRHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDER
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSHRDSLNLKEIKLEHPLPSIKPY
                     ISYNKSSLNNL"
BASE COUNT         3712 a         3982 c         3759 g         3774 t
ORIGIN      
        1 ggagaaaaaa gatatacctc gatcaccccc ctttctccca agtgaaaata aaaggttatc
       61 agtttgcaac attgaacaac attcgttgca aatcgataac aacatgcacc ttcaggatac
      121 tatttattat gttcggcaat gatattttca cccgcgtaaa acgttcagaa aataaaaaaa
      181 tggcggaaat cgcccaattc ctgcatgaaa atgatttgag cgttgacacc acagtcgaag
      241 tatttattac cgtaacccgc gatgaaaagc ttatcgcgtg cggtggaatt gccggaaata
      301 ttattaaatg cgttgctatc agtgaatccg ttcgcggtga aggactggcg ctgacattag
      361 ccactgaact gataaacctc gcctatgagc ggcacagcac gcatctgttt atttatacca
      421 aaaccgaata cgaggcgctg ttccgccagt gcggtttttc cacgttgacc agcgtaccag
      481 gcgtgatggt gctgatggaa aacagcgcca cgcgattgaa acgctatgcc gaatcgctaa
      541 aaaaatttcg tcatcccggg aacaagattg gctgcattgt gatgaacgcc aatcccttta
      601 cgaagggtca ccgttatctg attcaacagg ctgcggcaca gtgcgactgg ttgcatctgt
      661 ttttagtcaa agaagattct tcacgcttcc cctatgaaga ccggctggat ctggtgttaa
      721 aaggcaccgc cgatattcca cgcctgactg tgcatcgcgg ctccgaatac atcatctccc
      781 gcgctacgtt cccttgctac ttcattaaag aacagagcgt cattaaccat tgttacaccg
      841 aaattgattt gaagattttc cgtcagtacc tcgctcccgc gctgggtgta actcaccgct
      901 ttgtcggtac tgaacccttt tgtcgcgtta ccgcccagta caaccaggat atgcgctact
      961 ggctggaaac gccgactatc tccgcaccgc ccatcgaact ggttgaaatt gagcggctgc
     1021 gttaccagga gatgccgata tccgcttccc gggtacgtca actgctggcg aaaaacgatc
     1081 tcacggctat cgcgccgctg gtccctgcag tcacgctgca ttatttgcag aacctgcttg
     1141 agcactcccg ccaggacgcg gcagctcgtc aaaagacccc cgcatgagaa acaggtgaaa
     1201 aatgaaaata aaccagcccg ccgttgcagg cacccttgag tctggggatg tgatgatacg
     1261 catcgcccca ctcgatacgc aggatatcga cctgcaaatc aatagcagcg ttgagaaaca
     1321 gtttggcgat gcaattcgca ccaccattct ggacgttctc gcccgctaca acgtgcgcgg
     1381 cgtacagctg aatgtcgatg acaaaggcgc actggactgc attttacgtg cacgactgga
     1441 agccctgctg gcacgcgcca gcggtatccc ggctctgcca tgggaggatt gccaatgatt
     1501 tccgcttcgc tgcaacaacg taaaactcgc acccgccgca gcatgttgtt tgtgcctggt
     1561 gccaatgccg cgatggtcag caactcattc atttacccgg ctgatgccct gatgtttgac
     1621 ctcgaagact ccgtggcatt gcgtgaaaaa gacaccgccc gccgcatggt ttaccacgcg
     1681 ctgcaacatc cgctctatca cgatattgaa accattgtgc gtgtaaacgc gctggattcc
     1741 gaatggggtg ttaacgacct ggaagccgtc gttcgcggtg gtgcggacgt tgtgcgtctg
     1801 ccgaaaaccg ataccgctca ggatgttctg gatatcgaaa aagagatcct gcgtatcgaa
     1861 aaagcctgtg gtcgtgaacc cggcagcacc ggcctgctgg cggcgattga atctccgctg
     1921 gggattaccc gcgcagtgga aatcgctcac gcttccgagc gtttgatcgg tatcgccctc
     1981 ggtgcagaag actatgtgcg caacctgcgt acagaacgct ccccggaagg aactgaactg
     2041 ctgttcgcac gctgttccat tttacaggcc gcgcgctctg cgggtattca ggcgttcgat
     2101 accgtctatt ccgacgctaa caacgaagcc ggattcctgc aagaagccgc ccacatcaaa
     2161 cagctgggct ttgacggcaa atcgctgatc aacccgcgtc agattgatct gctgcacaac
     2221 ctctacgcac cgacccagaa agaagtggat cacgcccgcc gcgtcgtaga agccgctgaa
     2281 gccgccgctc gcgaaggcct cggcgtggtt tccctgaacg gcaagatggt ggacggtccg
     2341 gttatcgatc gcgcccgtct ggtgctctcc cgtgcagaac tttccggcat tcgcgaagaa
     2401 taaggcaatc aaaatgacgc agaaaattga acaatctcaa cgacaagaac gggtagcggc
     2461 ctggaatcgt cgcgctgaat gcgatcttgc cgctttccag aactcaccaa agcaaaccta
     2521 ccatgctgaa aaagcgcgcg atcgcaaact gtgcgccaac ctggaagagg cgattcgtcg
     2581 ctctggttta caggacggca tgacggtttc cttccatcac gctttccgtg gcggtgacct
     2641 gaccgtcaat atggtgatgg acgtcatcgc gaagatgggc tttaaaaacc tgaccctggc
     2701 gtccagctcc ctgagtgatt gccatgcgcc gctggtagaa cacattcgcc agggcgtggt
     2761 tacccgcatt tatacctctg gcctacgcgg tccactggcg gaagaaatct cccgtggtct
     2821 gctggcggaa ccggtgcaga tccactctca cggcggtcgt gtgcatctgg tacagagcgg
     2881 cgaactgaat atcgacgtgg ctttcctcgg cgtcccgtcc tgcgatgaat tcggtaatgc
     2941 caacggctac accggtaaag cctgctgcgg ctccctcggc tatgcaatgg tcgatgccga
     3001 caacgcaaaa caggtcgtga tgctcaccga agaactgctg ccttatccgc ataatccggc
     3061 aagcattgag caagatcagg ttgatttgat cgtcaaagtt gaccgcgttg gcgatgctgc
     3121 aaaaatcggc gctggcgcga cccgtatgac cactaacccg cgcgaactgc ttattgcccg
     3181 tagcgctgcg gatgtgattg tcaactctgg ctacttcaaa gaaggtttct ccatgcaaac
     3241 cggtaccggt ggcgcatcgc tggcggtaac ccgtttcctg gaagacaaaa tgcgtagccg
     3301 cgatattcgc gccgacttcg ccctcggcgg tattactgca acgatggttg acctgcacga
     3361 aaaaggtctg atccgcaaac tgctggatgt gcagagcttt gacagccatg ctgcgcaatc
     3421 gctggcccgt aaccccaatc acatcgaaat cagcgccaac cagtacgcta actggggttc
     3481 gaaaggcgca tcggttgatc gtctcgacgt ggtggtactg agcgcgctgg aaattgacac
     3541 ccagttcaac gttaacgtgc tgaccggctc tgacggcgta ctgcgcggcg cttccggtgg
     3601 tcactgcgat accgcaattg cctctgcgct ttccatcatc gtcgcaccgc tggtacgcgg
     3661 ccgtattccg actctggtgg ataacgtgct gacctgcatc accccgggct ccagtgtcga
     3721 tattctggtc acagaccacg gtatcgcagt taacccggca cgtccggaac tagcagaacg
     3781 tctgcaggaa gcgggcatta aagtggtttc cattgagtgg ctgcgcgaac gtgcgcgtct
     3841 gctgaccggt gaaccacagc cgattgaatt caccgaccgc gtcgttgccg ttgtgcgtta
     3901 ccgcgatggc tcggtgatcg atgttgtgca tcaggtgaag gaataagcca tgcacctgct
     3961 tcctgaactc gccagccacc atgcggtgtc aattcccgag ctgctcgtca gccgggatga
     4021 aaggcaagca cggcaacacg cctggctcaa gcgccatcct gttccactgg tctcctttac
     4081 cgtggttgcg cctgggccga ttaaagacag cgaggtcaca cgccgaattt ttaatcatgg
     4141 cgtgaccgcc ttgtgtgcct tagccgcaaa acagggctgg caaattcagg agcaggctgc
     4201 actggtttcc gccagcgggc cggagggcat gttaagcatt gccgccccgg ctcgcgacct
     4261 caagctcgcc accattgagc ttgaacatag tcatcctctc gggcggttat gggatatcga
     4321 tgtcctgacg cccgaaggcg aaattctctc ccgccgcgac tattcactgc cgcctcgccg
     4381 ctgcctgttg tgcgagcaaa gcgcagccgt ctgcgcgcgt ggaaaaaccc atcaactgac
     4441 cgatttactc aaccgcatgg aggcactgct gaacgatgtc gatgcctgca acgtcaacta
     4501 aaaccacaaa gcttgcgacg tcattaatcg atgagtacgc cctgctgggc tggcgcgcca
     4561 tgctgactga agtcaatctg tcaccgaaac caggcctcgt ggatcggatt aactgcggtg
     4621 cgcacaaaga tatggcgctg gaagatttcc accgcagcgc gctggcaatt cagggctggt
     4681 taccccgttt cattgaattt ggtgcctgta gtgcagaaat ggcaccagaa gcggtactcc
     4741 acgggttacg cccaattggt atggcttgcg aaggtgatat gttccgcgct actgcgggcg
     4801 taaacacgca taaaggcagc attttttctt tagggctgct atgtgcggca attggccgtt
     4861 tgcttcagct caaccagccg gtaacgccaa caaccgtttg ttctacggcg gcaagtttct
     4921 gccgtggcct gaccgatcgc gaactgcgta ccaataattc acaactgacg gcaggtcaac
     4981 ggttgtacca gcagcttggc cttaccggcg cacgcggtga agccgaagcg ggttatccac
     5041 tggtgatcaa ccacgccttg ccgcattacc tcactctgct ggatcagggg ttagatcctg
     5101 aactggcatt gctcgatacc ttgctcctac tgatggcgat caacggcgat accaacgttg
     5161 cgtcgcgcgg tggcgagggg ggcctgcgct ggctacagcg cgaggcgcaa acattattgc
     5221 aaaaaggggg cattcgaacc cccgccgatc tcgattatct ccggcagttc gacagggagt
     5281 gtatcgaacg aaatctcagt ccaggcggca gtgccgacct gctgatcctt acctggtttt
     5341 tagcacagat ttaattattt aaacacttga taaattttga aatattaatt ttcggagaac
     5401 ccgtatgtct ttagcaaaag ataatatatg gaaactattg gccccactag tggtgatggg
     5461 cgtcatgttt cttatccctg tccccgacgg tatgccgccg caggcatggc attacttcgc
     5521 tgtgtttgtg gcaatgattg tcggcatgat cctcgagcca attccggcaa cagcgatcag
     5581 ttttattgcg gttactattt gcgttattgg cagtaattac ctgctctttg atgccaaaga
     5641 attagctgac ccagcgttta atgcgcaaaa acaggcgctg aaatggggtc tggctggttt
     5701 ttccagcacc acggtatggc tggtatttgg cgcatttatt tttgcattag ggtatgaagt
     5761 ttccgggtta ggtcgtcgca ttgccctttt cctggtgaaa ttcatgggca aacgcacgct
     5821 gacgttgggt tatgcgattg tcattatcga cattctgctg gcaccgttta caccgtccaa
     5881 caccgcgcgt accgggggta cggtttttcc ggtcattaaa aacctgccgc cgttgtttaa
     5941 atcattcccg aacgatccgt ccgcgcgtcg tattggcggc tatttgatgt ggatgatggt
     6001 cattagtacc agtctgagtt cgtccatgtt tgtcaccggt gcggcaccaa acgtgctggg
     6061 tctggagttc gtcagcaaaa ttgccggtat ccagattagc tggttgcagt ggttcctctg
     6121 cttcctgccg gttggggtta tcttgcttat cattgcgccg tggctttcct acgtgctgta
     6181 caaaccggaa atcacacaca gtgaagaagt ggcaacctgg gcgggtgatg aactgaaaac
     6241 catgggtgcg ctgacacgca gagagtggac actgattggc cttgtattgc tcagcttagg
     6301 tttgtgggta tttggcagtg aagtcattaa tgctactgcg gttggtctgc tggcagtttc
     6361 gctaatgctg gccctgcacg ttgtgccgtg gaaagacatt acccgctata acagcgcatg
     6421 gaacacgctg gtcaacctgg caactctggt tgtgatggct aacggcctga ctcgttctgg
     6481 ttttattgac tggttcgccg gtaccatgag tacgcacctg gaaggattct caccaaacgc
     6541 aacggtgatt gtactggttc tggtgttcta ctttgcacac tacctgtttg ccagcctgtc
     6601 tgcgcacacc gcaaccatgc tgccggttat tctggccgtc ggtaaaggga tcccgggcgt
     6661 accaatggaa caactgtgta tcctgctggt gctgtctatc ggtatcatgg gctgtctgac
     6721 gccgtatgca accggtcctg gggtgattat ttacggctgt ggctatgtga aatcaaaaga
     6781 ttactggcgt cttggcgcaa tcttcggggt gatttacatc tctatgttgc tgttggttgg
     6841 ctggccgatt ctcgccatgt ggaactaata cgttcgccaa acgctgaata aatcatcacg
     6901 cccgccaggt aagcccacct ggcgggcttt tttatgattt aatagatagt attcagtgta
     6961 tgtatgagtt ccacacccat tatgaaagca ttctggcgta acgccgcgtt gctcgcggtt
     7021 tctctgcttc ccttctcttc tgccaacgcc gtagcgttgc aggcaaaaca gtatggcgat
     7081 tttgatcgct atgtcctggc cctctcctgg caaaccggat tttgccagag tcaacacgat
     7141 cgaaatcgta acgaacgaga tgaatgtcgc ctgcaaaccg aaacgaccaa caaagctgat
     7201 tttctgaccg tacatggtct gtggccagga ttgcctaaat cggtcgctgc ccgtggtgtt
     7261 gatgaacgcc gctggatgcg cttcggttgc gctactcgcc caatcccgaa tctaccagaa
     7321 gcgcgcgcca gccggatgtg ttcatcgccg gaaaccggat tatcactgga aacggccgct
     7381 aaactaagtg aagtcatgcc tggagctggc ggacgttcct gcctggaacg ctacgaatat
     7441 gccaaacacg gtgcctgctt tggttttgat ccggacgcat acttcggtac gatggtacgc
     7501 ctgaatcaag aaattaaaga gagcgaagcc ggaaaattcc ttgcggataa ttacggtaaa
     7561 acagtgagcc gccgtgactt tgacgccgcc tttgccaaaa gctggggaaa agagaacgtg
     7621 aaagcagtta agctaacgtg ccagggtaac cctgcgtatt tgactgaaat tcagatctcg
     7681 atcaaagctg acgccatcaa cgctccgctt tctgcaaact cattcttgcc acaacctcac
     7741 ccaggtaact gtggcaaaac ctttgtgatt gataaagcgg gttattaatc ctctgattta
     7801 caaaacacgc taattcgtct tccataacca aggcgattaa gatttataca taatgcgctg
     7861 cattgaggaa ttccctgaat gtgacgcaaa tcacttcaaa gagtgagcaa cagtcagtat
     7921 catcatgtga gttaaaccct cgccgcctga cggtgagggt tttcttttgg gtatgtttat
     7981 ctgcgacact cgcagtaccg acgacatgga gtaaaaatgt ccagaccaac tatcatcatt
     8041 aacgacctgg atgctgaacg catcgatatt ctgctggagc aacccgccta tgctggtttg
     8101 ccaatcgccg acgcgttaaa cgcagagttg gatcgcgccc aaatgtgttc gccagaagag
     8161 atgccacacg acgtggtgac aatgaacagc cgggttaaat tccgcaatct tagcgatggc
     8221 gaagtgcgtg tgcgcacgct ggtgtatccg gcaaaaatga ccgatagcaa tactcagctt
     8281 tccgttatgg ctccggtagg tgccgcactg ctggggctgc gcgttggcga ttccattcac
     8341 tgggaacttc cgggcggcgt tgcaacccac cttgaagtgc tggaactcga ataccagcca
     8401 gaagctgctg gcgactacct gctttaatcc catctgcaca agcgataact cgctttgccc
     8461 agaggatgca ctcgcatcct ctttacgcaa aatcagcaat ttttcctggt tctcactgtt
     8521 cacttttgct gcaaaattat tccgtttcgt ttgtgggaat aaccatggca gaagcatttt
     8581 acatcctgat aggatttctg attatggcgg cgattatcgt catggctgtc ctttacctcg
     8641 aaaaccactc ctgacgatca tattgttccc cccggcatcg cattcaccag acctgaaacc
     8701 gccttctgcg ccgcctctgc gctttgtgca ccgggtacca gaatcacctt acggcactcc
     8761 tcttcacgtt tttcgaaaat ctcatatccc cgggcggcct cttcaaacgg catatagtgg
     8821 gtaacaattt cttctggttt cagtaatcct ttctcaatta acggtaataa ttctcccagc
     8881 catgcgtgaa cgtgggtctg tcccatttta aacgtcaacc ctttatcaaa ggcgtcgcca
     8941 aacaggaaac cgtgaataaa tccagcgtag acgcccggta cgctaacaat gccgccacgc
     9001 ctgaccgccg caatacactg gcgcaacgct ttaccgctgc tgccctccag tttcaggtta
     9061 gtcagcaccg tttccgtggt gctgcctttc gcttcaaaac cgacggcgtc tattactgca
     9121 tccacgcccc ggtgacctgc cgtttgttca ataattgact gtgccggatc gctatcttca
     9181 tcgaaattaa tcgggatcgc gctgtagcgg tcagcggcga aacgcaagcg gtagggatga
     9241 tgatcaacaa caaaaatctg ttccgcaccg agcaaccgtg cacaggcgat tgtcaacaat
     9301 cccacaggac cagcaccata gactgcaacg cttgaacctt gttggatctg cgcatttttt
     9361 gctgcctgcc atgccgttgg cagaatatca gaaaggaaaa gcgctttatc atctgaaagc
     9421 aaaggcggta ctttaaacgg ccccacattc cctttaggga cgcggacata ttccgcctgc
     9481 ccaccaggaa cgccgccata caggtgacta taaccaaaca atgccgctgg agctggtatc
     9541 tgttttttat tgagcgcagc gcctttaccc gcattggtat tttcgcaggc ggcatattgt
     9601 tgcaatcgac agaaaaaaca gtcgccacaa gcaatgacga acggaattac cacccggtcg
     9661 cctttttgca aattttttac gtcctttccg gtttcaacta cttcccccat aaattcatga
     9721 ccaaaaatat cgccatgttt aacctgaggt attttgcctc gataaagatg gaggtcagag
     9781 ccacagatcg ccgttgccgt aatacgcaga ataatatcat ctgcctgttc aacgcccgga
     9841 tcgggaacat tttctacctg aacgtgatgt gggccgtgat aagtcaatgc tttcatgcaa
     9901 cctccagaga cagttcaaaa aagagtcagt ctttaagggt agctatcaca gcgggaaaac
     9961 gggcggtacc acaggttttg ggagaattcc tggtgttcag tgagaatata ttcatcacag
    10021 aaacagttcg taaggaaaaa gaaccagaca aatatcaggg ataaatatta taatcatggc
    10081 gaatgatttg ttcatgatta acagggagaa agggttatgt ataagacaat cattatgcca
    10141 gttgatgtat ttgaaatgga attgagcgac aaagctgttc gccacgctga attcctcgcc
    10201 caggatgacg gagttattca tctacttcac gtactacccg ggtcagccag cctgagcctg
    10261 caccgttttg ccgctgatgt gcgtcgtttt gaagagcatc tgcaacatga agcagaagaa
    10321 cgtctgcaaa cgatggtcag ccacttcacc atcgatcctt cccgcattaa acaacatgtc
    10381 cgttttggta gcgtgcggga tgaagtcaat gagttggcag aagaactggg ggctgatgtt
    10441 gtagttattg gttctcgcaa cccatcgatt tcgacccatc tgttaggttc taacgcctcg
    10501 agcgtgatcc gccacgccaa tctgcctgta ctggtcgttc gctaacagat aaaagtattt
    10561 atgccgccct gcctggaaca tcccggcagg gctttttaat gccataaaaa agccgccagg
    10621 tttgaccccg gcggataagc aattgcaggt gaatcttact tcttcttatg cagttttggt
    10681 gcgaatcagg tagtcaaaag cactcagaga ggctttggca ccttcgccag tggcgatgat
    10741 gatctgcttg tacggaaccg tcgtacagtc acccgctgcg aatacgcctt tgacgttggt
    10801 ttcgcatttc gcatcaatga taatctcacc catgcggtta cgttcgactg cgccttccag
    10861 ccagttggtg ttcggcagca gaccaatctg gacgaaaata ccggccagtt cgatgttgtg
    10921 aatatcgccg ctgacacgat ctcgatattc cagaccaacg actttgctgc cgtcgccttt
    10981 cacttccgtg gtttgcgcat tcagaataat gtcgacgttt ttcaggctgc gcagtttgtc
    11041 ctgcagcacc tggtcggctt tcatttctgg cgcaaattcc agcagagtta cgtgctcaac
    11101 gatacccgcc aggtcgattg ccgcttccac gccggagtta ccgccgccga taaccgctac
    11161 gcgtttgcct ttaaacagcg ggccgtcgca gtgcgggcag taggtcacgc ctttggtgcg
    11221 atactgatct tcgcccggaa cgttcatgtt gcgccatttt gctccggtcg ccacgataat
    11281 gctgcgtgct ttcagtaccg cgccagaagc tgtttcaatc tgatgcagac caccttcaac
    11341 cgctgccggg atcagtttgc tggcgctctg gctgtcgatc acatcaacgt cgtattcatc
    11401 aacgtgaact ttcagtgcac ctgccagttt ctggccttca gtcttcggta cagaaatgta
    11461 gttttcgata tcaacggtat cgaggatctg accaccaaaa cgttcgccca tcagaccggt
    11521 acggatgcct ttacgtgcgg agtaaattgc tgccgctgca cccgccgggc cggaaccaac
    11581 gattaatacg tcataagcat cacgcttgtt cagctcttct gccgcacgtt tttccgcgcc
    11641 agtatcaatt ttggcaacga tttcagtcaa cgtcatgcgg ccctgaccaa actctttccc
    11701 gtttacgaac actgccggaa cgcccatcac gttacgatcg gtgatttcgt tctggaaggt
    11761 gccgccatca attgcagtgt gcttgatgcg cgggttcagt acgctcatca ggttcagcgc
    11821 ctgcaccacg tccgggcagt tgtggcaaga gagcgagtaa taggtttcga attcaaaatc
    11881 accgtcaata tggcgaatct gctccagcag agactgcgct tctttcgacg gatgaccacc
    11941 ggtccacagc aacgccagta ccagcgaggt gaactcgtgg cccagcgggg agcctgcaaa
    12001 acgtggcccc tggttagaac ctgggttggt gatcaggaaa gacggcttac gcaccggcaa
    12061 gctgttatct tctttaaagg tgactttgtc tgacagttct gcgatttcag ccaacagttc
    12121 cttgatttct gccgatttag cgctgtcatc cagcgtggca attaactcaa caggcttggt
    12181 caatttctca aggtaagcct tgagttgagt tttcatattt gtgtcgagca tgtttatctc
    12241 ctgggcttta aacatcatca tgcaagcggc ctctgcggct acctggatgc agcttgcatc
    12301 atggtgcggg gaagaaaatc gggtgccgca gcacccgaag aattaggtga atttccgaag
    12361 tatttcgcgt cagagcaagg cggctagcct gtgaatcccc gggagcttac acaagtaagt
    12421 gaccggggtg agcgggcgac gccaacgccg ctatggcgtg aaagacgaag gaaatttaga
    12481 ttttaccaac caggtccaga gacggagcca gagttgcttc accttctttc catttagccg
    12541 ggcaaacttc acctgggtga gaagctacgt actgtgctgc tttgatttta cgcagcaggt
    12601 cagacgcgtc acggccaatg ccttcagcgg taacttcgat tgcctggatg ataccctgcg
    12661 ggtcaacaac gaaggtcgca cggtcagcca gaccttcatc ttcacgcatg ttgtcgaagt
    12721 tacgggtcag ggcgccagtc gggtcgccga tcatcgcata tttgatttta gcgatggttt
    12781 cagagctgct gtgccatgct ttgtgggtga agtgagtatc ggtagatact gcgtatacgt
    12841 ctacgcccag tttctgcagt tcttcgtagt ggtcagcaac gtcacccagt tcggtcgggc
    12901 atacgaaagt aaagtcagcc gggtagaaga agaagacgct ccagcggcct tcggtatctt
    12961 tttcggtgat ttcgatgaat tcgccgtttt tgaatgcctg gtttttaaaa ggtttaattt
    13021 tggtgttaat caaggacatc tatacttcct ccgtgttttc gatgagatgt aaggtaacca
    13081 atttttgccg attcggctaa tgcgtttcca ttatcaaatc gataagtttt accttacaac
    13141 cttcgtaaga caactttgtg aactttgcac cgccagcggc gataaaaagt aaaatggccc
    13201 cctttccagg tgaccgtgta atctgaacta ccctttggta tcttcagcgc ctgcctgcag
    13261 gctgaggtgt tgcccttaca aatgcaacaa cgacatggat tacaacaccc tcataaacaa
    13321 agggcaatca cctgatctaa gctcttacct atgacagtga taggttatgc cttttactcg
    13381 acttttgcac tgactgaaaa ggacaaatta atgttaaaaa agatactttt actggctctg
    13441 cttcctgcaa tcgccttcgc agaggaactt cctgctccag taaaagcgat tgaaaaacag
    13501 ggcattacaa tcatcaaaac gttcgatgcc cccggaggaa tgaaaggtta tctcggaaag
    13561 tatcaggata tgggcgtcac catctacctg actccagatg gtaagcacgc tatctctggt
    13621 tacatgtaca acgagaaagg tgaaaacctg agtaacacac ttatcgaaaa agaaatttac
    13681 gcaccagccg gacgcgaaat gtggcaacgg atggaacaat cccactggct cctcgacggt
    13741 aaaaaagatg cgccggtcat tgtctacgtc ttcgccgatc cgttctgccc atattgtaaa
    13801 cagttctggc agcaggcgcg cccgtgggta gattctggca aagtacagtt gagaacattg
    13861 ttggttgggg ttatcaaacc agaaagtccg gcgacagccg cggcaattct tgccaccaaa
    13921 gatcccgcaa aaacctggca agaatatgaa gcctctggtg gcaagcttaa gctaaacgtg
    13981 tctgcaaacg taagtacaga gcaaatgaaa gtgttaagcg acaacgagaa actcatggac
    14041 gatctggggg caaatgtcac tccggctatc tattacatga gtaaggaaaa tacgctgcaa
    14101 caggccgtgg ggttgcccga tcagaaaacg cttaatatca ttatggggaa taaataaaag
    14161 ataactcatg gatggttgaa atatatctct gcttttcaac catcctaaaa aagcatgaat
    14221 tgctcgctct gccctaagtg gctgtgctat tttagaaaaa cttaatcctg agttaagcaa
    14281 aaaaacataa tcaataaaaa tacatacgca aaaaacattg attaagcgaa tataccatgg
    14341 aagaaaaata taaaccggag tagtgtatgg ccaatctcta cgacttgaaa aagttcgact
    14401 taaatcttct tgtcatattt gagtgtattt accagcattt aagcattagc aaagctgctg
    14461 aatcactata tatcacccca tcagccgtga gccagtcgtt acaacgctta cgtgcgcaat
    14521 ttaatgaccc tttattcatt cgctcaggaa aaggtatcgc gccgacgaca acagggttga
    14581 atctgcatca ccatcttgag aaaaatctca gaggccttga acaaacaatt aatatagtca
    14641 ataagtccga actaaagaaa aactttatta tctacggccc tcagctcatt tcttgctcta
    14701 ataatagtat gctgatccgc tgcctacggc aagatgcctc tgtcgagatt gagtgccatg
    14761 atatcctcat gtcagcagag aatgctgaag aactgttggt tcaccgcaaa gccgatctgg
    14821 tgatcaccca aatgccagtg atcagccgtt ccgtcatttg catgccattg catacgattc
    14881 gcaatactct catttgtagt aataggcatc cacgaattac tgacaatagc acttatgaac
    14941 aaattatggc agaagagttc acccaattaa tatctaaatc agcaggggtt gatgatattc
    15001 aaatggaaat agacgagaga tttatgaatc gcaaaatttc atttcgtggt tcctctttgc
    15061 tgacaatcat taatagcatt gcagttacag atttactggg aatagtcccc tatgaacttt
    15121 ataactccca ccgtgactct cttaatttaa aagagataaa actagaacat ccgttacctt
    15181 caataaagcc ttacatttcc tataataaat cttctttaaa caatctg
//
LOCUS       DAPGOF010000110        15138 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.111, whole genome shotgun sequence.
ACCESSION   DAPGOF010000110 DAPGOF010000000
VERSION     DAPGOF010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15138
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.111"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            68..340
                     /locus_tag="RHV13_003101"
     CDS             68..340
                     /locus_tag="RHV13_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HDV1242614.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     DYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(341..1213)
                     /gene="rluF"
                     /locus_tag="RHV13_003102"
     CDS             complement(341..1213)
                     /gene="rluF"
                     /locus_tag="RHV13_003102"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HDV1242615.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKETPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKTKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1561..2506
                     /locus_tag="RHV13_003103"
                     /pseudo
     CDS             1561..2506
                     /locus_tag="RHV13_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
     gene            2576..3382
                     /locus_tag="RHV13_003104"
     CDS             2576..3382
                     /locus_tag="RHV13_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV1242616.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGKYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3392..3799
                     /locus_tag="RHV13_003105"
     CDS             3392..3799
                     /locus_tag="RHV13_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HDV1242617.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNTNVEAVEFVPGENAGDIV
                     AKLKKLVSIHSQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            3799..4293
                     /locus_tag="RHV13_003106"
     CDS             3799..4293
                     /locus_tag="RHV13_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HDV1242618.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAIAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4359..5156
                     /locus_tag="RHV13_003107"
     CDS             4359..5156
                     /locus_tag="RHV13_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HDV1242619.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAVPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQAPASTALDQLDD"
     gene            5167..5991
                     /locus_tag="RHV13_003108"
     CDS             5167..5991
                     /locus_tag="RHV13_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDV1242620.1"
                     /translation="MEQRKITRGDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPVKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VIMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            6043..7272
                     /gene="sorE"
                     /locus_tag="RHV13_003109"
     CDS             6043..7272
                     /gene="sorE"
                     /locus_tag="RHV13_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HDV1242621.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTNNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            7654..8343
                     /gene="pepE"
                     /locus_tag="RHV13_003110"
     CDS             7654..8343
                     /gene="pepE"
                     /locus_tag="RHV13_003110"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HDV1242622.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8433..10064)
                     /locus_tag="RHV13_003111"
     CDS             complement(8433..10064)
                     /locus_tag="RHV13_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDV1242623.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(10284..13967)
                     /gene="metH"
                     /locus_tag="RHV13_003112"
     CDS             complement(10284..13967)
                     /gene="metH"
                     /locus_tag="RHV13_003112"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDV1242624.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWEVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRAWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARIRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGEE
                     AKRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            14167..14991
                     /gene="iclR"
                     /locus_tag="RHV13_003113"
     CDS             14167..14991
                     /gene="iclR"
                     /locus_tag="RHV13_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HDV1242625.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         3573 a         3981 c         3988 g         3596 t
ORIGIN      
        1 tattatcagt tctgctcgca aacgaattcc cgtacactga actctctggc tcactgaggg
       61 caccatcatg gcactccccc gtattaccca aaaagagatg actgaacgcg agcagcgtga
      121 acttaaaaca ctgctggatc gggcgcggat tgcgcatggt cgcgtattga ccaactccga
      181 aaccaacagc attaagaaag attacatcga taaattaatg gttgagcgtg aggctgaagc
      241 aaaaaaagcc cgccaattga agaaaaagca ggcttataaa ccggatcctg aagcgtcatt
      301 tagctggtca gcaaatacgt cgacgcgcgg caggcgctga ttagcgcccc ttctttttac
      361 gccccggcga ggtaaaacgc ttaccgttgg acgccgggcg accgcctttt tccgccgttt
      421 tttccatctt aacgactgga cgtttgatgc ccgccgtttt cggtttggcc ttcgttttag
      481 gttttgcctc ggaagaggaa ttttcgatga gcttaaagag atcgataagc tcgtcgtcgg
      541 ttaaatcgcg ccattctccc agcggaatgc cgcttaagct gacgttcata atgcgcgtac
      601 gttccagctt tttcacttca tagccgaaat gctcgcacat gcgacggatc tgacggttca
      661 gcccctgcac cagggtaatg cggaagacaa acggagtttc ttttttgact ttgcactttt
      721 tcgtcaccgt cccgaggatc ggcactcccg cccccatacc acgaataaat tcatcggtaa
      781 tcggtttatc gaccgtcacc agatactctt tctcatgatc gttacccgca cggaggatct
      841 tattcaccag atcgccgtga ttggtgagga aaatcagccc ctgggagtct ttatccaggc
      901 ggccaatcgg gaacacgcgt ttgctgtggt taacgaaatc gacaatgtta tcgcgctcgc
      961 catcttcggt ggtgctgaca ataccgacgg gcttgttcag ggcgataagt accaaatctt
     1021 cggcttcccg aggctcaatc aactgaccgt ttactttcac aacgtcgccg ggtttcacct
     1081 gatcgccaat ggtggctcgc ttgccattaa ggaacacatt gccttgctcg atatagcgat
     1141 ccgcttcgcg gcgtgagcaa attccgcttt cgctgatgta tttatttaaa cggactgatg
     1201 agtcgggcag cataaattct cctgtaaaac gcggaatata ccgtacctct tagcggggaa
     1261 aaacagggtt atgcggcaac gtcgaaatta aatgtgatct gccacgcctt ttatcacaaa
     1321 agtgtctccg gaagtacaat gcaaatttgc ctggcaaaat aaggataatc aacatattac
     1381 gaatcgctgc acacctgtgc tgatgactac tgcgcagaga aacaaatgtg caagagaaag
     1441 cgctatttct ccccatgcaa agagtgaaaa atcgctttga aaggccatca tgatggggtt
     1501 atatcagatc aagcggtgag aaagcgtatt tgcacaaatg tgcagaacca ggaggcggca
     1561 atggaaaaca gtgacgatat ccgtttgatt gtgaagattg cccaactcta ttacgaacag
     1621 gatatgacgc aggcgcaaat cgcgcgcgaa ctgggtattt accgcaccac catcagccgc
     1681 ttgcttaaac gaggccgcga tcagggaatt gtcaccatcg ccatcaacta tgactacaac
     1741 gaaaatctct ggctcgagca gcaactgaag caaaagtttg gcctgaaaga cgttgtggtg
     1801 gtgtcgggaa atgatgagga tgaagagact caactggcga tgatggggtt acacggcgca
     1861 caactgctgg atcgcttgct ggagcctggc gatattgtcg gtttttcctg gggtcgcgcg
     1921 gtgagcgcac tggttgaaaa cttgccgcaa gcggggcaat cgcggcagtt aatctgcgta
     1981 ccaattattg gcggaccgtc cggtaaactc gagccgctat cacgtaaaca cattaaccta
     2041 cagcgcggca gcgaagctga aaggggaatc gcatctcgcg gattttccgg ctctactgga
     2101 taacccatta attcgtaatg ggatcatgca gtctcagcac tttaaaacca tctctgccta
     2161 ctgggataat ctggatgtcg ccctggtggg aattggctca ccggccattc gcgacggcgc
     2221 taactggcat gcgttttatg gtggtgaaga gagtgacgac ctgaatgccc gccaggttgc
     2281 tggcgatatt tgctcgcgct tttttgatat tcacggcgca atggttgaaa cgaatatgag
     2341 cgaaaaaaca ctctctatcg aaatgaataa attaaagcag gcgcggtatt ccattggcat
     2401 tgccatgagt gaagaaaaat acagcggaat tgttggtgca ctgcgtggaa aatatattaa
     2461 ttgtctggta acgaatagca gcacagctga actattactg aaataaaaat cttcccgatg
     2521 attatcgtaa taaaaattac ggcgggtccc tattatctaa aatcaggagt ctgttatgca
     2581 aacgtggtta aatttgcagg ataaaatcat tatcgtcacc ggcggcgcat ccggtattgg
     2641 tctggctatt gtagaggaat tattagcaca aggcgcgaat gtacagatgg tagatattca
     2701 cggtggcgat ggacaatatg aaggtcataa aggctatcag ttttggccga ccgatatttc
     2761 cagcgccaaa gaggtaaatc atacggtagc ggaaattatc cagcgttttg gtcgcatcga
     2821 cggtctggtc aataacgccg gggtcaattt cccgcgtctg ctggtcgatg agaaagcgcc
     2881 tgccgggaag tatgaactca acgaagctgc attcgaaaaa atggtcaata tcaaccagaa
     2941 aggcgttttt ctgatgtcgc aggcggtggc gcgacagatg gtcaaacaac atgatggcgt
     3001 gattgtgaat gtttcctcag aaagtggact ggaaggctca gaaggacaaa gctgttacgc
     3061 cgctaccaaa gccgcgctca atagcttcac gcgctcctgg tcgaaagagc tgggtaaaca
     3121 cggtatccgt gtggtcggta tcgcgccggg gattctggaa aaaaccgggc tacgcacgcc
     3181 ggaatatgaa gaggcgctgg cgtggacgcg caatatcacc gtcgagcagc tgcgtgaagg
     3241 ctataccaaa aacgccattc ctattgggcg cgccggaaga ttagcagaag tcgctgattt
     3301 tgtttgttat ctgttgtctg aacgcgccag ctacatcacc ggagtaacca ctaacattgc
     3361 gggcggcaaa acgcgcggat aaggaggctt tatggtcaac gccatttttt gtgcccacgg
     3421 caaattagct tgcgccatgc tggaatcggt tcagatggtt tacggcaaca ccaatgttga
     3481 agccgttgaa tttgtgcccg gtgaaaacgc cggagacatc gtagcaaagc tgaaaaagtt
     3541 agtaagcatt cacagtcaag atgagtggct gattgccgtg gatttacagt gcggcagccc
     3601 gtggaatgcg gcagcgatgc ttgcgatgcg caatcctcgc ctgcgggtaa tcagcggact
     3661 ttctctgccg ctggcgctcg aactggttga taaccaggac agcatgaatg ttgatgaatt
     3721 gtgtgaacac ctgacgcaaa tcgcaaagca aacctgcgtt gtctggaaac aactggcaac
     3781 gacagaggag gatttctgat gaacattacg ctcgcccgca ttgatgaccg cctgatccac
     3841 ggtcaggtca ctaccgtctg gtcgaaagtg gctaacgccc agcgcattat tatctgcaat
     3901 gacgaagttt ataacgatga agttcgccgg acattattgc gtcaggctgc gccaccggga
     3961 atgaaggtta acgtggtcaa tattgaaaaa gccatcgcgg tttatcacaa cccgcaatat
     4021 caggacgaaa ccgtttttta tcttttcact cgcccacagg atgccttagc gatggttcgc
     4081 cagggggtaa aaataggcac cctgaatatt ggcggcatgg cctggcggcc cggtaaaaaa
     4141 cagttaacca aagcggtttc attagatgat gatgatatta acgcatttca tgagttaaat
     4201 aatcttggcg tcatactgga tttacgcgtc gtagcgtcag atccctcaat caatattatc
     4261 gacaaaataa acgaacagtt aatcgcaaat taaataatag cgcctgtgta catatgcttt
     4321 aacaggcagg gatgacgaca cttaaaaggt gacaaatcat ggaaataagt accctacaaa
     4381 ttattgctat atttcttttt tcctgtattg ccggaatggg cagcgtgctg gatgaattcc
     4441 agactcatcg cccgttaatt gcctgtacgg tgattggttt aattctcggt gatttaaaaa
     4501 ccggaataat actcggtggt acgctggaat tgatagctct cggctggatg aacgtcggcg
     4561 cggcgcaatc tccggattct gcactcgcca gcataatctc cgccattctg gttatcgttg
     4621 gtcagcaaag catcgccacc ggaatcgcca tcgcgttgcc tgtggctgcg gcaggccagg
     4681 tgctgacggt gtttgcacgt accatcaccg tagtgttcca gcacgcggcg gataaagcag
     4741 cggaagaagc acggtttcgc actctcgata ttctgcatgt ctccgcgctt ggcgtgcagg
     4801 cgctgcgcgt tgctgttccg gcactgattg tctcgctgtt cgtcagcgcc gatatggtca
     4861 gcaatatgtt aaacgccatt cccgaatttg tgacccgagg actacagatt gctggcggtt
     4921 ttatcgtggt ggtcggttac gccatggtgc tacgtatgat gggcgtgaaa tatttgatgc
     4981 ctttcttttt cctcggtttc ctcgcaggtg gctacctcga tctcagtctg ctggcgttcg
     5041 gtggcgtcgg cgtgatcatg gccctggtct acatccagct aaatccacag tggcgtaaag
     5101 ctgaacctca actccaggcc cccgcttcca ccgcccttga ccaacttgac gattaacgga
     5161 gccatcatgg aacagagaaa aattacacgc ggcgatctgg tgagcatgtt tctgcgctcc
     5221 aacctgcaac aggcgtcctt taactttgaa cgtattcacg ggctgggttt ttgctacgac
     5281 atgatccccg ccatcaagcg actttaccca gtaaaagagg atcaggttgc ggcgctcagg
     5341 cgacacctgg tgttcttcaa taccacgcca gccgtatgtg gcccggtcat cggagtcact
     5401 gccgccatgg aagaggcgcg ggccaacggc gcggaaattg atgacggtac cattaacggc
     5461 atcaaagtcg gtctgatggg accgctggca ggggttggcg atccactggt ctggggaacg
     5521 ctgcgcccga ttaccgccgc gctcggcgca tctctggcac tttcgggcaa cattctcggc
     5581 ccgctgctgt tcttctttat tttcaacgcg gtgcgtctgg cgatgaagtg gtatggccta
     5641 cagctcggct ttcgcaaagg ggtgaatatc gtcagcgata tgggcgggaa tgtgctgcaa
     5701 aaactcaccg aaggcgcgtc gattctcgga ctgtttgtga tgggcgtgct ggtcaccaaa
     5761 tggacgtcaa tcaacgtacc gttggtggtt tcacaaacgc atgccgccga tggctccacc
     5821 gtcatcatga ccgtgcagaa cattctcgac caactttgcc ctggtttgct ggcgctcggt
     5881 ctgacgctac tgatggttcg tctgctcaac aaaaaaatta acccggtatg gctgattttc
     5941 gccctgtttg gcttagggat tatcggcaat gcgctgggct tcctgtcctg attcttcgcc
     6001 ccggcacgac tgccggggcc atcgctcaac atgaggtggt ttatgaaaac aacagctctg
     6061 cgtctttatg gtaaacgtga tttacgcctg gaaacctttg accttcctga aatgcaggag
     6121 gatgaaatcc tcgcgacggt ggtcactgac agcctgtgcc tctcttcctg gaaagaggcc
     6181 aacctgggtg aaaaccataa aaaagtaccc gacgatgtgg cgaccaaccc aatcatcatc
     6241 ggccacgagt tttgcggcga tattctggcc gtgggtaaaa agtggcagca caaattccag
     6301 ccgggtcagc gttatgtgat tcaggccaac ctgcaactcc ccgaccgccc ggactgcccc
     6361 ggctactcct tcccgtgggt aggtggcgaa gccacacatg tggttattcc caacgaggtc
     6421 atggaacaag attgcctgct ggcatacgac ggcgaaacct attttgaagg ctcgctggtt
     6481 gaaccgcttt cctgcgtgat tggcgcgttc aacgccaact atcatcttca ggaaggtagt
     6541 tataaccaca cgatggggat tcgcccgcaa gggcgcacgc tgatcctcgg cggcaccgga
     6601 ccaatgggac tgttggcgat tgattatgcg ctacatggac ccgttaaccc gtcgctgctc
     6661 gtcattaccg ataccaacaa cgataaattg agttatgcgc gcaagcacta tccatcagaa
     6721 ccgcaaacac tgattcatta tctcaatgcc accgatgcag catttgatac gttaatggcg
     6781 ctgagtggcg gtcacggctt cgatgatatt ttcgtctttg tgcctaatga aggactggtg
     6841 actctcgcct cttccttgct ggcgacagat ggttgcctga atttcttcgc cggaccgcag
     6901 gataaacatt tcagcgcgcc aattaatttc tacgatgtgc attatgcatt tacccactac
     6961 gtgggcacgt caggcggcaa taccgacgac atgcgcgcgg cggtcaaact gatcgaagag
     7021 aaaaaagtgc aggccgcaaa agtggtaaca catattcttg ggctgaatgc cgcgggcgaa
     7081 accacgcttg aattgcctgc cgtcggcggc ggcaaaaagc tggtgtatac cgggaaatac
     7141 ctgccgctga cgtcactcac gcagattcag gatcaagcac tggcggcgat tctggcgcgt
     7201 catcagggga tctggtccgg tgaggcggag caatatctgc tcactcatgc agaggcaatt
     7261 tcccatgatt aatcgcgcta cttaactctg catgtcgctg gctggtcgcc gcggcaactt
     7321 cgcgacctga tgtgcggctg attgctgaag cggcccaatg tgcacgagaa ggataaaaaa
     7381 acatcagcgc cttaaggggc gctcaaattg gtggcaaagt gcacattgtt tatgccggat
     7441 gcggaatgaa gccttattcg gcctacataa tctcgaaaat tcaatatatt gcagttatct
     7501 ggtaggccag gtaagcgtag cgcatcaggc aatgctgtgt ttgtcatcag tctcagcgcc
     7561 tgtgcaatac gtgacatcgt gagccatgtc agtaagtggt taaattcgca gtggtagcat
     7621 gccttccgtg atttcttact aaccggagaa gtaatggaac tgcttttatt gagtaactcg
     7681 acgctgccgg gtaaagcctg gctggaacat gcactgccgc tgattgctga acagttgcag
     7741 ggtcgccgct cagcggtgtt tatccctttc gctggcgtaa cgcagacttg ggatgattac
     7801 acagcgaaaa cggctgcggt tcttgctccg ctgggtgttt ctgtcaccgg tattcatagc
     7861 gttgtcgatc ccgttgccgc gattgaaaat gctgagatcg tgattgtcgg cggcggtaat
     7921 actttccagt tgctgaaaca gtgccgcgag cgcgggctgc tggcaccaat tactgacgtg
     7981 gttaaacgtg gcgcactgta tattggctgg agcgcaggcg ctaaccttgc ttgcccaacc
     8041 attcgtacca ccaacgatat gccgattgtc gatccgcaag gtttcgatgc gctgaatctg
     8101 ttcccgctgc aaatcaaccc gcacttcacc aacgcgctgc cggaaggcca taaaggtgaa
     8161 acccgtgaac agcgtattcg cgaactgctg gtcgtcgcgc cagaactgac gattattggt
     8221 ctgccggaag gtaactggat cacagtgagt aaaggtcacg ctacgctggg tggcccgaac
     8281 accacttatg tgtttaaggc tggtgaagaa gcggttccgc tagaagctgg tcaccgtttt
     8341 taagtgaatt gccggatgca tgatccggca tcttgttcat agtgcctgat gtgacgcttt
     8401 acgcgtctta tcaggcctac agggtcacgg tattaatact cgtcccgtcc ttcatcttca
     8461 tccggctgtt ccagcacact gtaagccacc gaacaaaaca gcgagttcag acgctgcata
     8521 tcacccagta agcctaaatg tagcgaactg gtttcaatgc tctgcacgtt ttgctgatgc
     8581 aggcgatcga cgtgggcgtg ggaatagcgg cgattaagaa tgcgaaaacg atgtttgctg
     8641 cggcgcaaac gacgggcgct ggtaacatcg ccagagaaga aaaccgacat tgccagtttt
     8701 aaattactga gcaattgctc atagagcgca tccagttcct tcaagccatc aagcgaaaat
     8761 gctcgccttg cagccagcga tttgtcggca atttcgctcc ccatgcgctc gacgatatcg
     8821 gaggcctgtt caaggttgag cgacatttcg atgatctccg cccagcggcg cgactcttcc
     8881 tccgccaact cttcttttgg catccgcgcc agatacagct taatggcggt atagagaacg
     8941 ttgatatcat cggccagttt acgcagctct ttctcctgcc gtggctcgcc gtgcatcact
     9001 ttattcagcc cttccatcat ctgttccatg gcgtcgccaa tgcgcagggt ttcgcgcgcg
     9061 gcgttcgcca gagcaagcgt gggcgtatcc agcgcgctga catccagatg tttaggccgt
     9121 agctgggtat ccagttccgg ttcatcgcga ataatcgttt tgcaaaaccg tgccatcggg
     9181 tcaacaaatg gcagcatgac caggcaacgt acaaggttgt agaagacgtg gaaatagatc
     9241 accagttccg cttttggcaa tgacagcttc cccattgtct ctgccagcaa atggacaaac
     9301 ggcaggataa tcaggctacc caccagctta aacagcagac tacccagcgc gacacggcgg
     9361 gctgcggcat tggcggcact gttgttgagc atcgccagca ggccggaacc gaggttagca
     9421 ccaatcacca gacagagcgc cacggggaag gagataatgc ctgcggcggt cagagtcgcg
     9481 gtcagcagta cagcagcaag gctggagtag ctgataatgg cgaacatcgc gccaatcagc
     9541 gcatccagca gaatatcgcc ggtcagcgag gcaaagatca cctgaacgcc gtttgcctgg
     9601 gtgatcggcg ttacggcctg cacaatcaac tccagcgcca gtaaaatcag cccaagacca
     9661 ataccgacgc ggcccagttg cccggcgcgt gactgtttgc gtccgaggaa aaaaatcacg
     9721 ccgataaaaa taagtaacgg tgacagccag gataagtcga aggtgagaat acgcgccatt
     9781 agcgccgtcc cgacatctgc ccccagcaca atgaccagag ccggtgcgag ggctaccaga
     9841 tcctgagcga cgaacgaggt caccagcatg gtggtggcat tactgctctg taccagtgcg
     9901 gtaacgccga tccccgcgca aaaggcgagc ggcttttttt cgacgctccg gctaaggaca
     9961 gtacgcaaac gcgcgccgaa gacgcgcatt acgccggttc gaacaatatg agtcccccag
    10021 accagcaggg cgacggcaga aagcaggtga agcagcgtta acacgagttc aggttctcct
    10081 tatcgttata tgttcctgaa gcaattgaat gaccaaagga ccggaaacac gctgactggc
    10141 atctttactg tgccttttaa cattcgtttt tccggctaag attattccag cctgtttcca
    10201 gtataagtgt ttcatattaa taaagagaca gggcactcat tgagcgccct gtttgttgta
    10261 aggaaaagtg acagatttgt gaatcagtcc gcgtcatacc ccagattcgg tgccagccag
    10321 cgctcaacat ctgagacgct cataccttta cggcgggcat agtcttcaac ctgatcgcgc
    10381 tggatttgcg ccaccgcgta atacttgctg tccgggtggc tgaagtacca acccgaaacc
    10441 gatgcacccg gccacatggc gaaagattcc gtgagtttca taccggtatg tttttccact
    10501 tccagcagct cccagatggt ggctttttcc gtatgttccg ggcaggccgg gtagcccggt
    10561 gccggacgga tgccctggta gttttcgcgg atcagttctt cgttgctgag gttctcgttc
    10621 ggcgcatagc cccagtagac tttacgcaca cgctcatgga gatactccgc aaaggcttcg
    10681 gctaaacggt cggcaagcgc tttcaccatg attttgttgt aatcatcgtg ctgcgcttca
    10741 aaggcatcag ccagtgcgtc ctcttccagc ccgccagtca cggcaaatgc gccgatgtaa
    10801 tctgctttac cagaaagctt cggcgcaacg aagtcagcga gacagtagtt agcgaatccc
    10861 gttttttcgg tctgttgtcg tagatggtgg ctgacgttga tcacatgggt acgtgtttcg
    10921 tcgcggtaga tttcaatgtc atcgcccaca cggtttgccg ggaacaggcc caccacgcca
    10981 cgcggattca gcgttttctc ggcgcttaat ttatccaaca tgtcgttggc gtctttaaac
    11041 agccgcttcg cctcttcgcc taccacttca tcttccagaa tgcgcggata cttcccggcc
    11101 agcgaccagg tcataaagaa cggtgtccag tcgatgtaat tacgcagcgt ttcgatgctg
    11161 gcttcgactt cctgcacgcc gagacggtgc gccaccggcg gcgtgtaagc ctgccagtca
    11221 aaagcgaaat cgttatcgcg cgccgcttcc agcgtgaccg gtggtgtgcg tggtttcttg
    11281 cgcccgtgct gaatacgcac ggtttcgtac tccttgcgga tacgagcgac aaaatcatcg
    11341 cgctgagtat cggaaagcag cgccgccacc acaccaacgg tgcgcgaggc attctgcaca
    11401 tacaccgtcg ggccgctgta gttctgctca attttcaccg ccgtgtgcgc ttttgaggtc
    11461 gtcgcgccac cgatcagtaa cggaatggtg aagccctgac gctccatctc tttcgccacg
    11521 ttaaccatct cgtccagcga cggcgtgata agccccgaaa gtccaatcag atcagcattc
    11581 acttctttag cggtacggag aattttttcc gcaggcacca taacgccgag atcgacaatt
    11641 tcgtagttgt tacattgcag caccacacca acgatatttt taccgatgtc gtggacgtcg
    11701 cccttcacgg tggcgatcac catcttgccg ttggttttgc cctgctcttt gctggcttca
    11761 ataaacggtt cgaggtaggc caccgcctgc ttcatcacac gcgccgattt aaccacctgt
    11821 ggcaggaaca ttttcccttc gccgaacaga tcgccgacca cgttcatgcc gtccattagc
    11881 gggccttcaa tcacttcaat cgggcgcgta gcctgctggc gggcttcttc ggtatcctgc
    11941 tcgataaact cggtaatgcc tttgaccaac gagtactcca gacgtttatt cacttcccac
    12001 gcgcgccact ccgcctgctg ggcgttagcg gtgtcgtcgg ttttgctgcc gcgatatttc
    12061 tcggcaagct ccagtaaacg ctcggtgcca tcgtcgcgac gattaagaat cacatcttcc
    12121 accgcgtcgc gcagttccgc gggcaggtcg tcgtaaatcg ccagctgccc ggcgttgacg
    12181 atccccatat ccatgccatt gcgaatagcg tagtagagga acaccgcgtg aatagcttcg
    12241 cgcaccggat cgttaccacg gaatgagaaa gaaacgttag atacgccgcc ggaaatcagc
    12301 gcgtgcggca gttcgcgttt gatgtcttcg caggcgccga taaagtcctg cgcatagttg
    12361 ttgtgctctt caatgccggt ggcgaccgcg aagatgtttg ggtcgaagat gatatcttct
    12421 ggcgggaagc caacctcttc ggtgaggatt ttgtacgccc gacggcaaat ctcgattttc
    12481 cgtgcgcggg tatcggcctg tccctgttcg tcaaaggcca ttaccaccac cgctgcaccg
    12541 taacgacgca acagtttcgc atgatggata aaggcgtcga cgccctcttt cattgagata
    12601 gagttaacaa tgcctttgcc ctgaatacac ttcagcccct tttcgatgac ctcccatttt
    12661 gaggagtcga tcataatcgg cacgcgagcg atatccggtt caccggcaat cagattgaga
    12721 aaacgcacca tcgccgcttc ggcgtcgagc atcccttcat ccatattgat atcgataatc
    12781 tgtgcaccgt tttccacctg ctggcgggcc acatccagcg cctcgctgta tttctcttct
    12841 ttgatcaggc gcttgaactt agcggaaccg gtgacgttgg tgcgttcacc cacgttcaca
    12901 aacaggctat cttcgccaat gttcagcggc tccaggccgg acaaacggca ggctacggga
    12961 atttcaggca gtttgcgcgg cgctaatcct tctactgcac gactcatcgc tgcaatatgt
    13021 tgcggcgtgg tgccgcagca gccgccgacg atattgagaa agcccgcttg cgcccattca
    13081 cgtatctgtt ttgccatcgt gtcggcgtcg agatcgtact caccaaaggc gttgggcagc
    13141 ccggcgttcg ggtgggcggt gacgtagcat tccgcaatcc gtgatagttc ctgcacgtac
    13201 tggcgcagtt cgtctggccc cagcgcgcag ttgaggccaa aagtcagggc ttcggcatgg
    13261 cgcagggagt tgtaaaatgc ttcggtggtc tgtccggaga gcgtgcgccc ggaggcgtcg
    13321 gtgatggtgc cggagatcat aatcggcagc tcaacgccta acgcttcgaa ctccgttttc
    13381 accgcaaaga ccgccgcttt ggcgttaagg gtgtcgaaaa cggtttcaat caggatcaga
    13441 tccgcgccac cttccaccag cgctttggtg gattcgcgat aggccgccac cagctggtca
    13501 aaagtgatgt tgcggaatgc cggatcgttg acgtccggag aaatagacgc cgtgcggttg
    13561 gtcgggccga gaacaccggc aacgtagcgc ggtttctctg gcgtgcgcgc ggtccactca
    13621 tcagcacagg cgcgcgccag tttcgccgcc gcaaagttga tttccgccga cagggattcc
    13681 atctggtaat ccgccatcgc aatggtcgtg gagttgaagg tgttggtttc gatgatatcc
    13741 gcgcccgctt caaagtaggc gttgtggata gcggcgatca cttccggttt actgagtacc
    13801 agcaggtcgt tgttgccttt gaggtcgcat ggccagtcgg caaagcgttc accacgaaaa
    13861 tcggcttcgt tcagtcgata gctctggatc atggtgccca taccgccgtc cagcaccaga
    13921 atacgttcat ttaactgcgc acgcagttgt tccactttgc tgctcacact tgctcccgac
    13981 acgctcaacc cagatttaat aaaaattcaa caaaccatac tggcataaac gcatctgtgg
    14041 taaaagcgac caccacgcaa catgagattt gttcaacatt aactcatcgg atcagttcag
    14101 caactattgc attagctaac aataaaaatg aaaatgattt ccacgataca gaaaaaggag
    14161 actgtcatgg tcgcacccat tcccgcgaaa cgcggcagaa aacccgccgt tgccaccgca
    14221 ccagcgactg gacaggttca gtctttaacg cgtggcctga aattactgga gtggattgcc
    14281 gaatccaatg gcagtgtggc actcacagag ctggcgcaac aagccgggtt acccaattcc
    14341 acgacccacc gcctgctaac cacgatgcaa cagcagggtt tcgtgcgtca ggttggcgaa
    14401 ctgggacact gggcaatcgg cgcacatgcc tttatggtcg gcagcagctt tctccagagc
    14461 cgtaatttgt tagcaattgt tcaccctatc ctgcgcaatc taatggaaga gtctggcgaa
    14521 acggtcaata tggcggtgct tgatcaaagc gatcacgaag cgattattat cgaccaggta
    14581 cagtgtacgc atctgatgcg aatgtccgcg cctatcggcg gtaaattacc tatgcacgct
    14641 tccggtgcgg gtaaagcctt tttagcccaa ctgagcgaag aacaggtgac gaagctgctg
    14701 caccgcaaag ggttacatgc ctatacccac gcaacgctgg tgtctcctgt gcatttaaaa
    14761 gaagatctcg cccaaacgcg caaacggggt tattcatttg acgatgagga acatgcgctg
    14821 gggctacgtt gccttgcagc gtgtattttc gatgagcacc gtgaaccgtt tgccgcaatt
    14881 tctatttccg gaccgatttc acgtattacc gatgaccgcg tgaccgagtt tggcgcgatg
    14941 gtgattaaag cggcgaagga agtgacgctg gcgtacggtg gaatgcgctg actttttctg
    15001 gcgggcagag gcaatattct gcccgtcata cctgagtggt aatagaataa gggtgtctgt
    15061 taatcgcatt gacgccaaaa taacttattg tcatacactt cacacacttt tattaactca
    15121 gcattatttt taaacatc
//
LOCUS       DAPGOF010000111        15102 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.112, whole genome shotgun sequence.
ACCESSION   DAPGOF010000111 DAPGOF010000000
VERSION     DAPGOF010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15102
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.112"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            185..427
                     /locus_tag="RHV13_003114"
     CDS             185..427
                     /locus_tag="RHV13_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242626.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            541..1179
                     /locus_tag="RHV13_003115"
     CDS             541..1179
                     /locus_tag="RHV13_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HDV1242627.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGASWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1176..1913
                     /locus_tag="RHV13_003116"
     CDS             1176..1913
                     /locus_tag="RHV13_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HDV1242628.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGKQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1913..4009
                     /locus_tag="RHV13_003117"
     CDS             1913..4009
                     /locus_tag="RHV13_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HDV1242629.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSVSDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQATTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(4056..4334)
                     /locus_tag="RHV13_003118"
     CDS             complement(4056..4334)
                     /locus_tag="RHV13_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242630.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4335..4478
                     /locus_tag="RHV13_003119"
     CDS             4335..4478
                     /locus_tag="RHV13_003119"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242631.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            4604..5014
                     /gene="psiE"
                     /locus_tag="RHV13_003120"
     CDS             4604..5014
                     /gene="psiE"
                     /locus_tag="RHV13_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HDV1242632.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(5058..6533)
                     /gene="xylE"
                     /locus_tag="RHV13_003121"
     CDS             complement(5058..6533)
                     /gene="xylE"
                     /locus_tag="RHV13_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HDV1242633.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYHIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6905..7795)
                     /gene="malG"
                     /locus_tag="RHV13_003122"
     CDS             complement(6905..7795)
                     /gene="malG"
                     /locus_tag="RHV13_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HDV1242634.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7810..9354)
                     /gene="malF"
                     /locus_tag="RHV13_003123"
     CDS             complement(7810..9354)
                     /gene="malF"
                     /locus_tag="RHV13_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HDV1242635.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTTQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIQPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9508..10698)
                     /gene="malE"
                     /locus_tag="RHV13_003124"
     CDS             complement(9508..10698)
                     /gene="malE"
                     /locus_tag="RHV13_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HDV1242636.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            11063..12178
                     /gene="malK"
                     /locus_tag="RHV13_003125"
     CDS             11063..12178
                     /gene="malK"
                     /locus_tag="RHV13_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HDV1242637.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12250..13590
                     /gene="lamB"
                     /locus_tag="RHV13_003126"
     CDS             12250..13590
                     /gene="lamB"
                     /locus_tag="RHV13_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HDV1242638.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEVNPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYTGNADTNANFGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13833..14753
                     /gene="malM"
                     /locus_tag="RHV13_003127"
     CDS             13833..14753
                     /gene="malM"
                     /locus_tag="RHV13_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HDV1242639.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     VLQQLTWTPVDQSKTQTTQLATGGQQLNVAGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
BASE COUNT         3732 a         3875 c         3975 g         3520 t
ORIGIN      
        1 agtttatgat ttttattgtt atttaaatat aagttgaaac ttatatttga tattcattcc
       61 aattatccta aaacgccatc gctaattccc cgcggcgtaa ttcgcatgct ttagttgtgt
      121 atactcgatc ccgcccgaaa tgtttttggg taaatctcca ttcattcaat gaagggaaat
      181 tgttatgaaa aaagttctgt atggcatttt tgccatatct gcgcttgcgg cgacttctgc
      241 gtgggctgca cctgtacagg tgggcgaagc ggcagggtcg gcagcaacgt cggtttcggc
      301 gggtagttcc tccgcgacca gcgtcagcac cgtaagctcg gcggtgggtg tcgcgctcgc
      361 ggcaaccggt ggcggtgatg gttctaatac cgggaccaca acaaccacga ccaccagtac
      421 ccagtaataa agtatgtatc cccaaaataa ttcgagtcat cgcatctgtg gcttgaagta
      481 tgaagggatt taccataacc acactccggt gtggttattc tgcccctctg gagaagagtc
      541 gtgaagcgac ctgcactcat tcttatctgc ctgctattac aggcctgttc agccacgact
      601 aaagagctgg gcaattcact gtgggacagt ctgttcggca cgccaggcgt acagctgacg
      661 gatgatgata ttcaaaatat gccctacgcc agccagtaca tgcagcttaa tggcgggccg
      721 cagttatttg tggtgctggc cttcgctgaa gacggacaac aaaaatgggt cactcaggat
      781 caggctactc tcgttaccca acatggccgt ctggtgaaga ctctgctcgg cggcgacaac
      841 ctgattgaag tgaataacct tgctgccgac ccgctgatta aacccgcaca aatcgttgat
      901 ggcgcaagct ggacgcgcac gatgggctgg accgagtacc agcaggtacg ctacgccacc
      961 gcacgctcag tcttcaaatg ggatggcacc gataccgtca aagtcggcag cgatgaaacc
     1021 ccggttcgcg tgctggacga agaagtctcc accgaccagg cgcgctggca taaccgctat
     1081 tggatcgaca gcgaagggca aattcgccag tcggaacagt atctcggcgc ggattatttt
     1141 ccggtgaaaa ccactctcat caaggcggca aaacaatgat taaacaaact attgtcgcgt
     1201 tgcttttgag tgtgggagcg tcatcggtct tcgcggcagg aaccgtcaag gtgttcagca
     1261 atggcagcag tgaggccaaa acgctaacgg gcgcagagca tttaatcgat ctggtaggcc
     1321 aaccgcggct ggcaaacagc tggtggcccg gtgcggtgat tagcgaagag ctggcaacgg
     1381 cggcagcatt gcgtcagcag caggcgttgc tgacacggct ggcagaacag ggcgcagatt
     1441 ccagcgccga cgatgccgct gcgattaacg ccttacgcca gcaaattcag gcgttgaaag
     1501 tgacgggcag gcaaaaaatc aatcttgatc ccgatatcgt ccgcgttgcc gaacgcggta
     1561 acccgccgtt gcagggcaac tacacgctgt gggtcggacc accgccgtcc acggtcacgt
     1621 tgttcgggct tatcagccgt cctggcaagc agccattcac tccaggtcgc gacgtggcga
     1681 gctatctctc tgaccagagc ctgctcagcg gtgcggatcg cagctacgcg tgggtggttt
     1741 acccggacgg acgcacgcaa aaagcgccgg tggcttactg gaacaagcgt cacgtagagc
     1801 cgatgcccgg cagcattatt tatgttggcc tcgcggactc cgtctggagt gagacgcctg
     1861 atgcccttaa cgccgacatt cttcagactc tgacgcagcg gatacctcaa taatgaaaaa
     1921 aagacatctg cttagcttac tggcgctggg cattagcaca gcttgctacg gcgaaacata
     1981 tcctgcgccc attggtccgt cgcagtcgga tttcggtggc gtaggattat tacaaacgcc
     2041 caccgcgcgt atggcacggg aaggggagtt gagtctgaac tatcgcgata acgatcagta
     2101 ccgttattac tcagcttcag tgcaactctt cccgtggctg gaaacaacgc tgcgctacac
     2161 cgacgtgcgc acccggcagt acagcagcgt cgaagcgttc tctggcgatc aaacgtataa
     2221 agataaagcc ttcgatctca aactgcgttt gtgggaagag agttattggc tgccgcaagt
     2281 ggcggttggt gcgcgggata tcggcggtac ggggctgttt gatgcggaat atcttgttgc
     2341 cagcaaagcc tgggggccgt tcgatttcac gctcggcctg ggctggggat atctgggtac
     2401 cagcggtaac gtgaaaaatc cgctctgttc ggtgagcgac aaatattgct atcgcgataa
     2461 cagctacaag caggcaggtt cgatcgacgg cagtcagatg ttccacggcc cggcatcact
     2521 gtttggcggc gtggaatacc agacgccctg gcaaccgctg cgtctgaaac tggagtatga
     2581 aggcaataat tatcagcagg attttgccgg gaagcttgag caaaaaagta agtttaacgt
     2641 cggggcgatt tatcgcgtta ccgattgggc cgacgttaac cttagctatg aacgtggcaa
     2701 cacctttatg tttggcgtta cgttgcgcac caactttaac gatctgcgtc cgtcttacaa
     2761 cgataacgcc cgcccgcaat atcaaccgca gccgcaggat gccattttgc agcattcggt
     2821 ggtggcgaat cagttaacgc tgttgaaata caatgctgga cttgccgatc cacagatcca
     2881 ggcgaaaggc gatacgctgt atgttaccgg cgagcaggtg aaatatcgtg attcgcgcga
     2941 agggatcatc cgtgcgaatc ggatcgtgat gaacgatctg ccggatggga tcaaaacgat
     3001 ccgcattacg gaaaatcgcc ttaacatgcc gcaggcgacg acggaaaccg atgtcgccag
     3061 cctgaaaaat catctcgccg gagagccgtt gggccacgaa acgacgctgg cgcaaaaacg
     3121 cgtcgagcca gtggttccgc agtccaccga gcagggctgg tatatcgaca aatcacgctt
     3181 tgatttccat atcgatccgg tgctgaacca gtcggtcggt ggcccggaaa acttttacat
     3241 gtatcagctg ggcgtgatgg gaacggcaga tttgtggctg acggaccatc tgctgaccac
     3301 cggcagcctg tttgcaaatc ttgccaacaa ctacgacaag tttaactaca ctaatcctcc
     3361 gcaggactcg cacttaccgc gcgtgcgtac ccatgtgcgc gagtatgtgc agaacgatgt
     3421 ctatgtgaat aacctgcaag ccaactactt ccagcatctg ggcaacggct tctacggtca
     3481 ggtctacggt ggttatctcg aaaccatgtt tggcggtgcg ggggcagaag tgttgtatcg
     3541 cccgctggac agcaactggg cgtttggtct ggatgccaac tacgttaaac agcgcgactg
     3601 gcgtagtgca aaagatatga tgaaattcac cgactacagc gtgaaaaccg gacatctgac
     3661 cgcctactgg acgccatctt tcgctcagga tgtgttagtt aaagccagcg tcgggcagta
     3721 tctggcaggg gataaaggcg gcacgctgga gatcgccaaa cgctttgata gcggcgtggt
     3781 ggtgggtggc tatgccacga tcactaatgt ttcaaaagag gagtacggcg aaggggactt
     3841 caccaaaggc gtgtatgtct ctgtaccgtt ggatctcttc tcgtctggcc cgacacgcag
     3901 ccgtgcggcg attggctgga cgccgctgac gcgtgacggt ggtcagcaac ttgggcgtaa
     3961 gttccagttg tatgacatga ccagcgaccg tagcgtcaat ttccgctaag tcatgggaaa
     4021 ggtgccagtt ttcgcaatca ctggcaccct cattcttaat ggcctgttga gccatcagga
     4081 acgcagttca cccccggttt gcgatctggt gtgtaaaggc gaaaacattc tgtcttcctg
     4141 tccgtcgata tcggttgcag gtctggttgc ctgagcgact gggccatcat tgggctggag
     4201 acattgcttt gcggtaggtc ggctttatca gcagtaccga gcggaccagc ataagcagga
     4261 agaacagaga ctgataacat caaagcagca aaataaggct tcatttttac cacctttatc
     4321 aggttacgtt tcatttgttc cagaggaaca ttgtcgattt ttcgcgcatt gctggtggct
     4381 gggaatcacc tgaatgggtg atttttgaat taccggcttt ggtgcggttt gtcttgccgg
     4441 atgcgccgcc aggcgcggct tatccggcct acgggtaggt atatccggct ttggtggagg
     4501 cgcgctccaa atccaggttg aacaaaacat acacaaaaaa tatagatctc cgtcacattt
     4561 ttgcgttata caggaagctc gccactgtga aggaggtact gctatgacgt cactctctcg
     4621 tccgcgcgtg gagtttatct ccaccatttt gcagaccgta ctcaatcttg gcctgctgtg
     4681 cctcggcctg attttggttg tcttcctcgg caaagaaacg gtgcatctgg ctgatgtgct
     4741 gttcgcgcca gaacaaacca gcaaatatga gctggtagaa ggactggtgg tttactttct
     4801 ctatttcgaa tttatcgcgc tgattgtgaa gtactttcag tccggttttc acttcccgct
     4861 acgttacttt gtctatattg ggatcaccgc cattgtgcgc ttgatcatcg tcgatcataa
     4921 atcgccactg gatgtgttga tctactcggc tgcgatcctg ttactggtga tcaccctgtg
     4981 gctgtgtaat tcgaaacggc tgaagcggga gtaaaaagac agcacgccga actggcgcgg
     5041 cgtgctggac aggaagatta cagcgtagca gtttgttgtg ttttcttcgt ttccggttcc
     5101 cagagggctt ccagctcctc aagggtttta cctttggttt ccgggacaaa tttccacata
     5161 aacagtgctg ccagaacgcc catacaaccg taaatccagt aggagaaacc gttgtggaaa
     5221 tgggccacaa gccaggagtt tttgtccatc atcgggaagg tccaggagac gaagtagttc
     5281 gccagccact gggccgccac cgcgattgcc agcgctttac cacgaatggc attcgggaag
     5341 atttccgaca gcagaaccca gcataccgga ccccaggaca tggcaaaggc ggcaacatag
     5401 aacagcatcg acagtagcgc cacaataccc ggtgcctgag tgtaaaacgc ggtaccgagg
     5461 ctaaacatac cgattgccat tccgagcgcg ccgataattt gcagtggctt acgaccaaat
     5521 ttatccaccg tcataattgc cagaacggtg aaggtgaggt tgataactcc gacaataatg
     5581 gtctgcaaca gcgcgatatc cgtgctggcc cccagcgttt tgaacacttc cggcgcgtag
     5641 tacagcacca cattgatgcc gacaaattgc tggaagatgg agagcataac gccgatgaca
     5701 atcacgccca cgccaaacat cagcagacga ccaccggttt tgcggccatg atccagggag
     5761 tgtttaattt cctgtactgc ctgagttgca agcgtgttgc ccataatttt gcgcaggata
     5821 ctttccgcct gttcttgctt gccgcgtgac atcagccagc gaggactttc tggcacggta
     5881 tacagcagca ttaagaacag cagtgcaggg atacattccg aggcaaacat ataacgccag
     5941 ccgtcagtat tcagccagct ggcatcaccg gaacgggcaa taaaatagtt tacgcagtaa
     6001 actaaaagtt gcccgaaaat aatcgcaaac tggttaaatg aaaccagttt tccgcgaata
     6061 tgagctggag ccagttccgc aatatacatt ggtgaaagca ttgaggctaa accaacgcca
     6121 ataccgccaa taatatgata aataacaaat tccgggacat aacctgccag ataaataggt
     6181 actgtgttgt ccgggtttat agaggtaaaa ccaagttctg gccaggcaga acctacacca
     6241 gaaataaaaa acaggacagc agcaatctta agtgaatcac gacggccgaa gcggttactg
     6301 caataaccac cgagggcacc gccgatgatg caaccaatca gagcgctggc tacgcaaaat
     6361 cctaacaggg agttggcagc ggattcactt aagttttgtg gagcaacaaa gacggtattg
     6421 agtgactcaa cagtaccgga aataacggcg gtgtcgtagc caaataataa accacctaat
     6481 gtagcgacta aggtaatcga aaatatataa ctggaattat actgggtatt cattcagacc
     6541 tgccttagac cattctgatc ttttatcagt gcattgatgt tggaattgtc aggcagttaa
     6601 ggcaattatc attttttaca atgcgtttac gtaatgtctt ttctgtgatc ttaattgtga
     6661 taattatcaa aaattgatga aaacaatact attgccgtga ctcagagcac gaaagagaat
     6721 tatcgtaagt gggaaaacaa ataacgtaaa aataataagc tctatgatgg gaatattaac
     6781 cggcgaacga ttcagattgc agacgaaaga aaaaaaggcg ctccgtggag cgccgaataa
     6841 cagtcacaag ttgggataac gtaagttgag ggtgcagcgg cataacattg gcagaacaac
     6901 atctttaacc tttcacacca cctgccgtca ggccgttgac cagccagcgt tgagccagca
     6961 agaagacgat ggtgatcggt aatgcagaca tcacggcagc ggcggcaaag tcaccccaca
     7021 ggtagttttg cgggttgagg tattgctgca tccccacggc cagggtgtag ctgtttacgt
     7081 cacgcagtaa cagcgacgcg accggaactt cagtaatggc agcgataaac gacaggatga
     7141 ataccaccgc cagaatcggt actgacagcg gcaacaggac aaggcggaag gcctgccacg
     7201 gtgtcgcacc atccagcgcc gccgcttctt ccagcgaact gtcgatggtt tcgaaatagc
     7261 ctttgatggt ccagacatgc agcgcaatcc cacccagata cgcgaaaatt acgccgccgt
     7321 gagtattcag gccaatgaat ggaatgtact cacccagacg atcaaacaac gcatacaacg
     7381 cgaccagtga aagtactgcc gggaacatct ggaaaatcag cattcctttc agcagcgtcg
     7441 ctttgcctgg aaagcgcata cgggcgaaag cgtaggcgca ggtggtggag agcgccacaa
     7501 tgccaatcgc ggaaatcccg gcgaccttta ccgagttcca cagccacagc agtaccggga
     7561 atggcggtgg cgtaatgcga ccatcagcct gttcaacgct aaaacctaac gccagtttcc
     7621 agtgatccca ggagatttgc tccgggatca ggctgccggt cgcaaagttc ccctggcgca
     7681 gtgagatagc gacgaccatc agcagcggga acataatcgc cgcgataaaa agtagcagta
     7741 gcaggtgagt aataaacaaa cgtgcttttt gcgatttcgg ttggaccatt gccatttttg
     7801 ttatctccct taatcaaact tcattcgcgt ggctttcagg ttcactatcg ccagcgcacc
     7861 caccagcagg aagatcagcg tggcaattgc tgccgccaga ccgaagtcct gacccccgcc
     7921 gccttcaaaa gcgatacggt aggtgtagtt aacaagcagg tcggtataac cggctggcgt
     7981 ggtcgtgcca agacgatccg ggccgccgtt ggttaacagc tgaatcagca cgaagttgtt
     8041 aaagttaaag gcgaagctgg cgatcatcag cggcgtcagc ggttgaatca gcagcggcag
     8101 cgtaatctta aagaagttct ggaacggacc tgcgccatcc attgctgagg cttcatacaa
     8161 atcgtccgga atcgctttca gcaagcccat gcagaggatc atcatgtacg gataacccag
     8221 ccaggtattg acgataatta gcatcgtgcg ggcggtggtc ggatcgctga accaggcggg
     8281 cttcacgcca aacagcgcgc tcaacatcat gttgatttca ccgaagctct ggttaaacaa
     8341 tcctttgaaa atcaagattg aaatgaacga tggcaccgcg tagggcagaa tcagcaggac
     8401 gcgatagacc gctttgccgc gcaacgcttc ccactgcacc agacatgcca gaaccatgcc
     8461 gaccgccacc gttaaaaaga cagtgatcag cgagaacacc acggtccaga cgaaaatggc
     8521 gaggaacggt ttctgaatac cttcgtcggt aaagacgcgg gtaaagtttt tccagccggt
     8581 ggtcacggtg taaccggggc ttagcttttc atcaccccag ttgccgtcgg cggtaatgga
     8641 ctggtaaaag ccaatttggt tattcggacg atatttcacg ccgctctgat tattcgtcaa
     8701 cgtgccgtca ccgtcgagtg tgtagagcgg ctgcgtgcca gaaaactggc gcagggagct
     8761 catcatcact ttgttgccat ccggcagaat ggcggtaatg tcactcagcg cctgacgatt
     8821 ctgggtaatc acgcgcagat tcgcgcgttc gccttcgggc tgggtggtcg tttctttcag
     8881 ttgcagtttt tgctcgccgc caaatttaaa agcgtcggag aggtaatttt tgccggtttc
     8941 gccgtcgctg agcgccagtt gccactcatc gcccgccggg taaagaccaa agttataggt
     9001 tttgcctgct tgccaggagc gatctaacaa cacttcctgt gcacgttcaa aagtcagctg
     9061 gttagtgctg ctgtagttgg tgaaggcaat ggcgatggtg cagaccagag ggaagaggac
     9121 gaataatccc attccagcca ttcccgggta aacatagcgc caggcgtagg ctttacgatt
     9181 ggcgaaaata tacagccccg ctgaactcaa tatcagcgtg gtaatggcga acaggtattc
     9241 cccttgtgcg tacattaaaa caacaaggta acccaccagc aggccgagca gacctaacac
     9301 tgaccatttc agcgcgtcgc tttgccacca atgtttcttt ttaatgacat ccatggggtt
     9361 cttcctcatt ccaggacgga taaggctttc acgccttatc cgacaacaac tgcctgatgc
     9421 gacgctgacg cgccttacca ggcctacata cgtttcggtt ttgtaggccg gacaaggcgt
     9481 tcacgccgca tccggcattt cacagcatta cttggtgata cgagtctgcg cgtctttcag
     9541 ggcttcatcg acagtctgac gaccgctggc ggcgttgatc accgcagtac gcacggcata
     9601 ccagaaagcg gacatctgcg ggatgttcgg catgatttca cctttctggg cgttttccat
     9661 ggtggcggca atacgtggat ctttcgccaa ctcttcctcg taagacttca gcgctacggc
     9721 acccagcggt ttgtctttat taaccgcttc cagaccttcg tcagtcagca gatagttttc
     9781 gaggaactct ttcgccagct ctttgttcgg actggcggcg ttaataccgg cgctcagcac
     9841 gccaacgaac ggtttagatg gttgaccctt gaaggtcggc agtaccgtta caccataatt
     9901 cactttgctg gtgtcgatgt tggaccatgc ccacgggccg ttgatggtca tcgctgtttc
     9961 gcctttatta aaggcagctt ctgcgatgga gtaatcggtg tctgcattca tgtgtttgtt
    10021 tttaatcagg tcaaccagga aggtcagacc cgctttcgcg ccagcgttat ccacgcccac
    10081 gtctttaatg tcgtatttgc cattttcata cttgaacgca taacccccgt cagcagcaat
    10141 cagcggccag gtgaagtacg gttcttgcag gttgaacatc agcgcgctct tacctttcgc
    10201 tttcagttct ttatccagcg ccgggatctc ttcccaggtt tttggcgggt tcggcagcag
    10261 atctttgtta taaatcagcg ataacgcttc aacagcgatc gggtaagcaa tcagcttgcc
    10321 gttgtaacgt acggcatccc aggtaaacgg atacagcttg tcctggaacg ctttgtctgg
    10381 ggtgatttca gccaacaggc cagattgagc gtagccacca aagcggtcgt gcgcccagaa
    10441 gataatgtca gggccatcgc cagttgccgc aacctgaggg aatttctctt ccagtttatc
    10501 cggatgctca acggtgactt taattccggt atctttctcg aatttcttac cgacttcagc
    10561 gagaccgtta tagcctttat cgccgttaat ccagattacc agtttacctt cttcgatttt
    10621 ggcgagagcc gaggcggaaa acatcatcgt cgttaatgcg gataatgcga ggatgcgtgc
    10681 acctgttttt attttcataa tctatggtcc ttgttggtga agtgctcgtg aaaacaccta
    10741 aacggactct agtttcttta tacggcaacc tctttccatc ctccttgccc ctacgcccca
    10801 ccgttgcttt gtgtgatctc tgttacagaa ttggcagtaa tgtggagatg cgcacataaa
    10861 atcgccacga tttttgcaag caacatcacg aaattcctta catgacctcg gtttagttca
    10921 cagaagccgc gttctcatcc tcccgcctcc tcccccataa aaaagccagg gggtggagga
    10981 tttaagccat ctcctgatga cgcatagtca gcccatcatg aatgttgctg tcgatgacag
    11041 gttgttacaa agggagaagg gcatggcgag cgtacagctg caaaatgtaa cgaaagcctg
    11101 gggcgaggtc gtggtatcga aagatatcaa tctcgatatc catgaaggtg aattcgtggt
    11161 gtttgtcgga ccgtctggct gcggtaaatc gactttactg cgcatgattg ccgggcttga
    11221 gacgatcacc agcggcgacc tgttcatcgg tgagaaacgg atgaatgaca ctccgccagc
    11281 agaacgcggc gttggtatgg tgtttcagtc ctacgcgctc tatccccacc tgtcagtagc
    11341 agaaaacatg tcatttggcc tgaaactggc aggcgcaaaa aaagaggtga ttaaccaacg
    11401 cgtcaaccag gtggcggaag tgctacaact ggcgcatttg ctggatcgca aaccgaaagc
    11461 gctctccggt ggtcagcgtc agcgtgtggc gattggccgt acgctggtgg ccgagccaag
    11521 cgtatttttg ctcgatgaac cgctctccaa cctcgatgct gcactgcgtg tgcaaatgcg
    11581 tatcgaaatc tcccgcctgc ataaacgcct gggccgcaca atgatttacg tcacccacga
    11641 tcaggtcgaa gcgatgacgc tggccgacaa aatcgtggtg ctggacgccg gtcgcgtggc
    11701 gcaggttggg aaaccgctgg agctgtacca ctatccggca gaccgttttg tcgccggatt
    11761 tatcggttcg ccaaagatga acttcctgcc ggtaaaagtg accgccaccg caatcgatca
    11821 agtgcaggtg gagctgccga tgccaaatcg tcagcaagtc tggctgccag ttgaaagccg
    11881 tgatgtccag gttggagcca atatgtcgct gggtattcgc ccggaacatc tactgccaag
    11941 tgatatcgct gacgtcatcc ttgagggtga agttcaggtc gtcgagcagc tcggcaacga
    12001 aacccaaatc catatccaga tcccttccat tcgtcaaaac ctggtgtacc gccagaacga
    12061 cgtggtgttg gtagaagaag gtgccacatt cgctatcggc ctgccgccag agcgttgcca
    12121 tctgttccgt gaggatggca ctgcatgtcg tcgactgcat aaggagccgg gcgtttaagc
    12181 accccacaaa acacacaaag cctgtcacag gtgatgtgaa aaaagaaaag caatgactca
    12241 ggagatagaa tgatgattac tctgcgcaaa cttcctctgg cggttgccgt cgcagcgggc
    12301 gtaatgtctg ctcaggcaat ggctgttgat ttccacggct atgcacgttc cggtattggc
    12361 tggacaggta gcggcggtga acaacagtgt ttccagacta ccggtgctca aagtaaatac
    12421 cgtcttggca acgaatgtga aacttatgct gaattaaaat tgggtcagga agtgtggaaa
    12481 gagggcgata agagcttcta tttcgacact aacgtggcct attccgtcgc gcaacagaat
    12541 gactgggaag ctaccgaccc ggccttccgt gaagcaaacg tgcagggtaa aaacctgatc
    12601 gaatggctgc caggctccac catctgggca ggtaagcgct tctaccaacg tcatgacgtt
    12661 catatgatcg acttctacta ctgggatatt tctggtcctg gtgccggtct ggaaaacatc
    12721 gatgttggct tcggtaaact ctctctggca gcaacccgct cctctgaagc tggtggttct
    12781 tcctctttcg ccagcaacaa tatttatgac tataccaacg aaaccgcgaa cgacgttttc
    12841 gatgtgcgtt tagcgcagat ggaagtcaac ccgggcggca cattagaact gggtgtcgac
    12901 tacggtcgtg ccaacctgcg tgataactat cgtctggttg atggcgcatc gaaagacggt
    12961 tggttattca cagctgaaca tactcagagt gtcctgaagg gctttaacaa gtttgttgtt
    13021 cagtacgcta ctgactcgat gacctcgcag ggtaaaggtc tgtcgcaggg ttctggcgtc
    13081 gcgtttgata acgaaaaatt tgcctacaat atcaacaaca acggtcacat gctgcgtatc
    13141 ctcgaccacg gtgcgatctc catgggcgac aactgggaca tgatgtacgt gggtatgtac
    13201 caggatatca actgggataa cgacaacggt accaagtggt ggaccgtcgg tattcgcccg
    13261 atgtacaagt ggacgccaat catgagcacc gtgatggaaa tcggctacga caacgtcgaa
    13321 tcccagcgca ccggcgacaa gaacaatcag tacaaaatta ccctcgcaca acaatggcag
    13381 gctggcgaca gcatctggtc acgcccggct attcgtgtct tcgcaaccta cgccaagtgg
    13441 gatgagaaat ggggttacga ctacaccggt aacgctgata ccaacgcgaa cttcggcaaa
    13501 gccgttcctg ctgatttcaa cggcggcagc ttcggtcgtg gcgacagcga cgagtggacc
    13561 ttcggtgccc agatggaaat ctggtggtaa tagcaaaacc tgggccggat aaggtgcttg
    13621 caccgcatcc ggcatccaac gcctgatgcg acgcttgcgc gtcttatcag gcctacaacg
    13681 gctgtcaaat gtaggccgga taaggcgttt acgccgcatc cggcataaaa acaggttgtc
    13741 attatctgaa aggggcgaaa gcccctctga ttatcgggtt tagcgcgcta ttgcctggct
    13801 accgctgagc tccagatttt gaggtgaaaa caatgaaaat gaataaaagt ctcatcgccc
    13861 tctgtttatc agcagggtta ctggcaagcg cgcctggaat cagccttgcc gatgttaact
    13921 acgtaccgca aaacaccagc gacgcgccag ccattccatc tgccgtgctg caacaactca
    13981 cctggacacc ggtcgatcaa tctaaaaccc agaccaccca actggcgacc ggcggccaac
    14041 aactgaacgt tgctggcatc agtggtccgg ttgctgcgta cagcgtcccg gcaaacattg
    14101 gcgaactgac cctgacgctg accagcgaag tgaacaaaca aaccagcgta tttgcgccta
    14161 acgtgctgat tcttgatcag aatatgaccc catcagcctt cttccccagc agttatttca
    14221 cctaccagga accaggcgtg atgagtgcag atcgtctgga aggcgttatg cgcctgacac
    14281 cggcgttggg gcagcaaaaa ctttatgttc tggtctttac cacggaaaaa gatctccagc
    14341 agacgaccca actgctcgac ccggctaaag cctatgccaa aggcgttggt aactcgatcc
    14401 cggatattcc cgatccggtt gctcgtcata ccaccgatgg cttactgaaa ctgaaagtca
    14461 aaacgaactc cagctccagc gtgttggtcg ggcctttatt tggttcttcc gctccagctc
    14521 cggttacggt aggcaacacg gcggcaccag ctgtggctgc acccgctccg gcaccggtga
    14581 agaaaagcga gccgatgctc aacgacacgg aaagttattt taataccgcg atcaaaaacg
    14641 ctgtcgcgaa aggtgatgtt gataaggcgt taaaactgct tgatgaagct gaacgcctgg
    14701 gatcgacatc tgcccgttcc acctttatca gcagtgtaaa aggcaagggg taattacgcc
    14761 ccacagtgct gattttgcaa caactggtgc gtctcctggc gcaccttttt ttatgcttca
    14821 ttccagggat atgagcgatt ttttatagta cttcatttct tcttcactaa gaatgtgcat
    14881 tatctcaatg ccttatcaga gattcttttc ctttcgccgg tagtgtctgg acgctcaggc
    14941 tactttttcc agttatttca tttctgtaat gcagaggttt tatgttaggt catatctcaa
    15001 agtttgacgg caataactct ttgataaaac atggtgtggt gcaaggaaat aatatagtcg
    15061 atcttgattt actacgtaat tttaatgggg tgccagggtt aa
//
LOCUS       DAPGOF010000112        15027 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.113, whole genome shotgun sequence.
ACCESSION   DAPGOF010000112 DAPGOF010000000
VERSION     DAPGOF010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.113"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            307..516
                     /locus_tag="RHV13_003128"
     CDS             307..516
                     /locus_tag="RHV13_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c1 repressor inactivator"
                     /protein_id="HDV1242640.1"
                     /translation="MAFIPPTIDDVRHCSNALSVDPAETDAARAIAEHYSKISNQEYR
                     ITQDDLDDLTDTIEYLMATNQPDSQ"
     gene            627..1478
                     /locus_tag="RHV13_003129"
     CDS             627..1478
                     /locus_tag="RHV13_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242641.1"
                     /translation="MINYVYGEQLYQEFVSFRDLFLKKAVARAQHVDAASDGRPVRPV
                     VVLPFKETDSIQAEIDKWTLMARELEQYPDLNIPKTILYPVPNILRGVRKVTTYQTEA
                     VNSVNMTAGRIIHLIDKDIRIQKSAGINEHSAKYIENLEATKELMKQYPEDEKFRMRV
                     HGFSETMLRVHYISSSPNYNDGKSVSYHVPLCGVFICDETLRDGIIINGEFEKAKFSL
                     YDSIEPIICDRWPQAKIYRLADIENVKKQIAITREEKKVKSAASVTRSRKTKKGQPVN
                     DNPESAQ"
     gene            complement(1503..2987)
                     /locus_tag="RHV13_003130"
     CDS             complement(1503..2987)
                     /locus_tag="RHV13_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HDV1242642.1"
                     /translation="MARSCVTDPRWRELVALYRYDWIAAADVLFGKTPTWQQDEIIES
                     TQQDGSWTSVTSGHGTGKSDMTSIIAILFIMFFPGARVILVANKRQQVLDGIFKYIKS
                     NWATAVSRFPWLSKYFILTETSFFEVTGKGVWTILIKSCRPGNEEALAGEHADHLLYI
                     IDEASGVSDKAFSVITGALTGKDNRILLLSQPTRPSGYFYDSHHRLAIRPGNPDGLFT
                     AIILNSEESPLVDAKFIRAKLAEYGGRDNPMYMIKVRGEFPKSQDGFLLGRDEVERAT
                     RRKVKIAKGWGWVACVDVAGGTGRDKSVINIMMVSGQRNKRRVINYRMLEYTDVTETQ
                     LAAKIFAECNPERFPNITIAIDGDGLGKSTADLMYERYGITVQRIRWGKKMHSREDKS
                     LYFDMRAFANIQAAEAVKSGRMRLDKGAATIEEASKIPVGINSAGQWKVMSKEDMKKK
                     LNLHSPDHWDTYCFAMLANYVPQDEVLSVEDEAQVDEALAWLNE"
     gene            complement(2987..4180)
                     /locus_tag="RHV13_003131"
     CDS             complement(2987..4180)
                     /locus_tag="RHV13_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HDV1242643.1"
                     /translation="MTWDDHKKNFARLARDGGYTIAQYAAEFNLNPNTARRYLRAFKE
                     DTRTTDSRKPNKPVRKPLKSMIIDHSNDQHAGDHISAEIAEKQRVNAVVSAAVENAKR
                     QNKRINDRSDDHDVITRAHRTLRDRLERDTLDDDGERFEFEAGDYLIDNVEARKAARA
                     MLRRSGADVLETTLLEKSLSHLLMLENARDTCIRLVQEMRDQQKDDDEGTPPEYRIAS
                     MLNSCSAQISSLINTIYSIRNNYRKESREAEKHALSMGQAGIVKLAYERKRENNWSVL
                     EAAEFIEAHGGKVPPLMLEQIKADLRAPKTNTDDEENQTASGAPSLEDLDKIARERAA
                     SRRADAALWIEHRREEIADIVDTGGYGDVDAEGISNEAWLEQDLDEDEEEDEEVTRKL
                     YGDDD"
     gene            complement(4266..4718)
                     /locus_tag="RHV13_003132"
     CDS             complement(4266..4718)
                     /locus_tag="RHV13_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001448129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Late promoter-activating protein"
                     /protein_id="HDV1242644.1"
                     /translation="MLLNWQGRHFMEINHSRITSYEIADYMIRTKSLLSAKELAAILE
                     KEYPHLDVDKRDVYLRLKAIAVSKYSSVLIDDSTRPRRFQIHSLNPEFFRRSRAPRRF
                     DEKLQNELYMTQDEKERREHQPWVMARQLFNKVARQHRHYGNATSARI"
     gene            complement(4807..5850)
                     /locus_tag="RHV13_003133"
     CDS             complement(4807..5850)
                     /locus_tag="RHV13_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HDV1242645.1"
                     /translation="MKAVITPFVQKELGLATFKVDQEVRKLVEAGRKFIMEPVPRELI
                     EHMEDGLVVTEQTMATNEALQPFFNSDELFRRIGGIDALVAWLRRKEGQCQAADRSWC
                     DNHIVHAERDNSAVLLCWHHDNHYRMRGFNELKETLHNNRVNWILDVARQEMGLSNSH
                     DLSIQELCWWAFMRNMMHLMPEEVCRISINKMKATPQDSGPLKEADIRPYDDRATAYV
                     QMMEERAAPMRAKVCPVDVDSDPGMAHFKIPKLQSLKLPEYMDFVASRPCCGCGAAGA
                     GAHITPYIVRHSRLCAHDIYAIPLCQSCQRDIERDRDNWEKTHGRLAMHQRLFFDYAL
                     GVGVITSHSSSVR"
     gene            complement(5878..6057)
                     /locus_tag="RHV13_003134"
     CDS             complement(5878..6057)
                     /locus_tag="RHV13_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PdcA protein"
                     /protein_id="HDV1242646.1"
                     /translation="MARKYNKLSREALKMLLDGVSRRKVKQYLVGKQIGARTAIAVLC
                     RQEMVVLKQRMPGSR"
     gene            complement(6062..6442)
                     /locus_tag="RHV13_003135"
     CDS             complement(6062..6442)
                     /locus_tag="RHV13_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HDV1242647.1"
                     /translation="MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVA
                     YEEITDLFEVSATYLVATARGHIFNDANKRTALNSALLFLRRNGVQVFDSPELADLTV
                     GAATGEISVSSVADTLRRLYGSAE"
     gene            complement(6442..6663)
                     /locus_tag="RHV13_003136"
     CDS             complement(6442..6663)
                     /locus_tag="RHV13_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDV1242648.1"
                     /translation="MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATF
                     EAYKKAALDAEFASLFDTLDSTNKELVNR"
     gene            complement(6736..7125)
                     /locus_tag="RHV13_003137"
     CDS             complement(6736..7125)
                     /locus_tag="RHV13_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HDV1242649.1"
                     /translation="MGFPSPAADYVESRISLDQQLIRHPSATYFMRAADSHHREGILQ
                     GALLVVDSSLTPVDGSLLVCAMEGEYRIKRYRKYPRQHLEDLSTGKKEALPVDDDGYT
                     GSNAVFGVITHVINDARSGEFDDCPVI"
     gene            7300..7884
                     /locus_tag="RHV13_003138"
     CDS             7300..7884
                     /locus_tag="RHV13_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDV1242650.1"
                     /translation="MPARIPLDPVLPKNFDCTPNEKRSKAQLDAWWDHPYGVTQPDGK
                     IVVYCLNGGAWDRPSVLCLADNYDEACELAERQQASWVKTRSEPTFMFSKEPPFILAR
                     MPQRPDHQQEIVAEFSSRDEINLFSLKQEERDRVEVSPTLDHNRMNLAQLAWYSKELE
                     MSIARLENEKAAIQVQHEVVLNRIREMQNDNRGF"
     gene            7885..8247
                     /locus_tag="RHV13_003139"
     CDS             7885..8247
                     /locus_tag="RHV13_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001062543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242651.1"
                     /translation="MAKIEYHRDRGNYLEIYDHESLNDINDALYEYCEKTSITDAPDA
                     FVELPVYLRDIYAIRTPPVSVIHIGYVRLSIEEDEDRYIVRHYTLDRKELPNEWNMSN
                     FYNGEYGLKPAKNGAGIA"
     gene            complement(8259..8498)
                     /locus_tag="RHV13_003140"
     CDS             complement(8259..8498)
                     /locus_tag="RHV13_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HDV1242652.1"
                     /translation="MSDWNIAAKSQEERDKVNVDLAASGVAYKERLNIPVIAEQVARE
                     QPENLRTYFMERLRHYRQLSLQLPKGNDPAYQKTE"
     gene            complement(8813..9571)
                     /locus_tag="RHV13_003141"
     CDS             complement(8813..9571)
                     /locus_tag="RHV13_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242653.1"
                     /translation="MTRITKERISEIIARIEMYGHGAGYTADEVLALAQMALAACKDD
                     KKMKLIDLLVKELPKCGGWPDGMIDCRLLSANLMAPCATFAFGSDHKKDTFFGRNFCC
                     EIELPIGDLDSDEYQSVVTREQYEAALIASQKVEFNGDEPESKAYKLDFEQWLEQQRG
                     KIDVDCGCVSAETFMHWLRVAYVAGNHPDFPDSSQQSPKKSVKTTLERGYLEAALKIK
                     PGHTLGVIDAMLVHEMAKALLPLVADKIPNKADI"
     gene            complement(9568..9942)
                     /locus_tag="RHV13_003142"
     CDS             complement(9568..9942)
                     /locus_tag="RHV13_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242654.1"
                     /translation="MMTLKHFLDRPLWAAAAGYDFNYMDCMSYTANAYDHSFSLLFNS
                     LRILPETEVGELHLWLLGFIAAVVGIAVWPFIFWLVAVVVWFKCKAYRKKYFLGDGMT
                     DIAKMNIEKWTKECEKKWRKKK"
     gene            complement(9949..10242)
                     /locus_tag="RHV13_003143"
     CDS             complement(9949..10242)
                     /locus_tag="RHV13_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000269003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242655.1"
                     /translation="MWRGNNHGKSQMILTEYKLDHKTNKSRSVYLLRHNSRVRNTVLE
                     QNLTVEMDNYGGFKPTISLDDFPRGLSEREAMLKLAEWLQRLSIAIEDNWSEP"
     gene            complement(10421..10654)
                     /locus_tag="RHV13_003144"
     CDS             complement(10421..10654)
                     /locus_tag="RHV13_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242656.1"
                     /translation="MGKLTFVVEFEDGKEPPVSANLDVAGGRLVSVLFGDYRDDFFQP
                     EEVDVVREALNELSVDNDDAHAEIIQKMELLTH"
     gene            complement(10737..11933)
                     /locus_tag="RHV13_003145"
     CDS             complement(10737..11933)
                     /locus_tag="RHV13_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HDV1242657.1"
                     /translation="MTTITKERLLTIKQWRETYGPGSNVVLPAEEAEELARIALASLE
                     AKPIGAFHIAEQQVDGTSDYLKDGEWPIDNGIIEVYAAPPVPVVPEEKPMPNPLSMYA
                     VDAVAAIAEVRGWNACRAAMLQSGNFRENKNSSTNNFREIAETSTNYPVIPSEVLSAI
                     LKVAKIRADFDDFDGDRRGIGDCLDEAEQELIVTINKYASQLAAEPIATNDVREQTAV
                     PPVPVIQADVAQAIEKLKRKLVECNRYNYCADAVKGVEYACHAAMLQGSQPVSQTYKF
                     PVNTPCQDAPAHIWLQTAGVWPEDGELSELTWCSHNQHHDDTLYVRADLVNGNSQVTP
                     DGWISCSERMPAQDDWILIYSKHGEYMAGQVQGEYVELSDGTLSWLGNALFWMLLPEP
                     PQEAGQ"
     gene            complement(11930..12139)
                     /locus_tag="RHV13_003146"
     CDS             complement(11930..12139)
                     /locus_tag="RHV13_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242658.1"
                     /translation="MAIAASYTMHLYCDCLQCTDGKYKSPDFGEYIGTSWAGCAKEAR
                     KDGWRISKDKTRAFAPGHKVLRINK"
     gene            complement(12141..12329)
                     /locus_tag="RHV13_003147"
     CDS             complement(12141..12329)
                     /locus_tag="RHV13_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242659.1"
                     /translation="MKNRKAKILLVRRNAPGVWQWVRLSNRRMGLMKYYGMMDCGFCK
                     KPSAAQNRWKNHLRTKGE"
     gene            complement(12675..13424)
                     /locus_tag="RHV13_003148"
     CDS             complement(12675..13424)
                     /locus_tag="RHV13_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HDV1242660.1"
                     /translation="MTIDYQALREAAERAIPAMEHLLMLPVDDDLLTEQELKDYGVDI
                     DALNAFKFLTGPETVLALLDERERNQQYIKCRDQENEDIALTVGKLRVELEEVKQHAE
                     ELSETKAVRNQWRPDICPITGRAFFMWIEHPTLGNVPTYGGPLDSYTIPTKDGDGEFS
                     CERYDHDFGGWVESECLGLYLIDDREQCRVYELEERVKELDAREISLPERSSMLHRTD
                     FHDDYQTVMAYKVSEVIDAIRAAGIRIKGGE"
     gene            complement(13421..14065)
                     /locus_tag="RHV13_003149"
     CDS             complement(13421..14065)
                     /locus_tag="RHV13_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009450597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242661.1"
                     /translation="MNNRFYMMCLRETVGNNASFHCHNGNGYSSDIDRAHVYTLEEAQ
                     KAWNCGRDIDQPVCADSVDAMAVWHVDCQYIPTESLIESDCTAYVAYKKGSWNGNDVY
                     WLQHGGLPTDDFSKATIFSVANKNEPGIVWLPFSIADAAKRRTFNINNFNRRTMVQGA
                     GLVMPDWLKEQNRRKKSRSGKVRWNCPHCGKITWQYSPYDFEGCSDYNCEGWRE"
     gene            complement(14058..14318)
                     /locus_tag="RHV13_003150"
     CDS             complement(14058..14318)
                     /locus_tag="RHV13_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242662.1"
                     /translation="MSESKCQINGNKIEPCAALAQSLEHDAEYTTRKGLLKYKIYNHE
                     LIHSQDLIMLRSGEFSKSPIRVSFCPFCGESLKTWEAEATSE"
     gene            complement(14311..14892)
                     /locus_tag="RHV13_003151"
     CDS             complement(14311..14892)
                     /locus_tag="RHV13_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242663.1"
                     /translation="MKGEVKERGMIFNDEMVRAILGGNKTQTRRIVEEKFYGRAVAAE
                     LLAKHCPYGQPGDRIWVRETYRVHGKATDVATLVYRASVRNSWTEQTHRVPVEVCNKP
                     VSEKWTPSIHMPRWASRILLEITNVRVERLNDISECDARAEGAPTESTLIGDKHYPGF
                     RSLWKSIYGEDSWNANPWVWVIEFERIQGATSE"
BASE COUNT         3806 a         3925 c         3199 g         4097 t
ORIGIN      
        1 aatcccactc caccagccaa aaactggatt gtttttcatt gttgtttgac aattgctcta
       61 ataaattata gttttgccgc cgtttcgtaa tacgactttg gattcactat ttaatgtgtc
      121 ttcagcgttg tagagcggct cagaaggaaa tgagcaaaca gggaaacctt atacaacggc
      181 attacagcta tgcattgctc atcttacaca cagcgcaatg ttgttagatt accccagcat
      241 ggatcatggg tgaaacagta ggtcagagct tcaggctctg tgttgtcaat acagtgaggc
      301 ataattatgg ctttcattcc accaaccatc gacgacgtta gacattgctc taacgcttta
      361 tctgtagacc ctgccgaaac cgacgctgcc cgcgccattg ctgaacacta ctcaaagata
      421 tccaatcagg agtaccgcat cacccaagac gacctggatg atctcactga cacaatcgaa
      481 tatctcatgg ccactaacca gccagactca caataaatgc actaataaat ctattatttt
      541 cgttggatcc ttctataatg gtggccaaca actcccagtg taatccgctg tgagttgttg
      601 gccatgtcaa ttctggagga ggatcaatga taaattatgt ctacggcgaa caactgtacc
      661 aggagttcgt cagcttcagg gatctctttc taaaaaaagc tgttgcacgc gcccaacacg
      721 ttgatgccgc cagcgacggt cgtcctgtac gcccggttgt cgttctgccg ttcaaagaaa
      781 cggacagcat tcaggctgaa attgataaat ggactttaat ggcgcgagaa ctggaacagt
      841 acccagacct caatatccca aagactattt tatatccagt gcctaacatc cttcgcggtg
      901 tgcgtaaggt tacaacttat cagacagaag ctgtgaacag cgtcaacatg accgctggcc
      961 gcattattca tctgattgat aaggacattc gcatccagaa aagcgcgggg atcaatgagc
     1021 acagtgcgaa atacatagag aacctggaag caacaaaaga gctaatgaag cagtacccgg
     1081 aggatgaaaa attccgtatg cgtgtacacg gctttagcga aacaatgctg cgcgtccatt
     1141 acatttccag tagccctaac tacaatgatg gtaaatcagt tagttaccat gtgccgctgt
     1201 gtggtgtgtt tatctgcgat gaaactctcc gtgatggaat catcatcaac ggtgaattcg
     1261 agaaagcaaa atttagcctt tatgactcta tagaaccgat catctgcgac cgctggccgc
     1321 aggcaaaaat atatcgcctg gcagatattg aaaatgtaaa aaaacaaatt gccatcactc
     1381 gcgaagagaa aaaggtcaaa tcagccgcat cagttacgcg cagccgtaaa actaagaagg
     1441 ggcagccagt aaacgacaac cccgaaagcg cgcaatagtt tctatccggc atggttaatg
     1501 agttattcat taagccatgc cagagcttca tcaacctgcg cttcgtcttc gacgctaagc
     1561 acttcatcct ggggaacata gttcgccaac atagcgaaac aatatgtatc ccaatggtcc
     1621 ggtgagtgca ggttgagttt tttcttcata tcttcctttg acatcacctt ccattgacct
     1681 gcggaattta tccctaccgg tatctttgat gcttcctcta tagtcgcagc ccccttatca
     1741 agcctcatac gccctgattt tacagcttct gccgcctgaa tattcgcgaa agcgcgcata
     1801 tcgaaataaa ggcttttatc ttcacggctg tgcatctttt taccccagcg gatacgctgg
     1861 acggtaatgc catagcgttc gtacattaga tcagccgtcg atttccccaa gccatcgcca
     1921 tcaatagcta tggttatgtt cgggaaccgt tctgggttac attctgcgaa aatcttggcg
     1981 gctaactgcg tttctgtaac gtctgtgtat tccagcatac gatagttgat tacacggcgt
     2041 ttatttcgct ggccggacac catcatgata ttaataacgg acttatctcg tcctgtgcca
     2101 ccagcaacgt caacacatgc aacccagccc catcctttgg caatcttgac ctttcgccgc
     2161 gtcgcccgct caacctcatc acgaccaaga agaaagccat cttgagattt gggaaattca
     2221 ccacgtactt tgatcatgta catggggtta tcacgaccgc catactccgc aagttttgct
     2281 cgtataaatt ttgcatctac aagcggagat tcttcactat tcagtattat cgcagtaaac
     2341 aatccatcag gatttcccgg gcgaatagct agtctgtggt gtgaatcgta gaaatagcct
     2401 gaaggtcgcg taggctggga aagaagcaga atacggttat ccttaccggt cagcgcacct
     2461 gttatcacac tgaatgcttt atcactcaca cccgacgctt cgtcgatgat atacaagaga
     2521 tgatcggcgt gttcaccagc caacgcctcc tcattcccgg ggcgacagga ctttatcaat
     2581 attgtccaaa cacccttgcc agtcacctca aaaaaagacg tttctgtaag aatgaaatac
     2641 tttgacaacc acgggaatct gctaacagca gtagcccaat tgctctttat gtatttgaaa
     2701 ataccatcaa ggacttgctg tcttttgtta gcgaccagaa tgacgcgagc gccggggaaa
     2761 aacatgatga agagtattgc aatgatactc gtcatatccg atttaccagt accatggccg
     2821 gaggtcacac ttgtccaact gccgtcctgc tgcgtggact caatgatctc atcctgctgc
     2881 caggttggtg tcttcccaaa caacacatca gcggccgcaa tccagtcata acgatatagc
     2941 gccacaagct cgcgccaacg tgggtccgtt acgcaacttc tggccattaa tcatcatccc
     3001 cgtacagttt gcgggtaact tcttcgtctt cctcctcgtc ttcgtccaga tcctgttcaa
     3061 gccatgcttc gtttgatatg ccttccgcat cgacatcacc ataaccacct gtatcgacga
     3121 tatcggcaat ttcttctcta cgatgctcaa tccacaatgc ggcatcagcg cggcggctgg
     3181 cggcccgttc tcgcgcgatt ttatccagat cttcaagtga tggagcgcca gatgctgttt
     3241 ggttttcctc atcatcggta ttggtcttag gagcacgcag atcggctttg atttgctcca
     3301 gcatcagggg aggcactttt cctccatgcg cctcgatgaa ttcagccgct tccagcactg
     3361 accagttatt ttcacgcttt cgttcgtatg ccagcttaac aatgccagct tgccccatag
     3421 ataaagcgtg cttttccgcc tcccggcttt cttttcgata gttattccgg atgctgtaaa
     3481 tggtgttgat caggctgctt atctgcgcgg aacagctgtt tagcatgctc gcgatacggt
     3541 attcaggcgg agtaccttca tcatcgtctt tttgctgatc gcgcatttcc tgcactaggc
     3601 gaatacacgt atccctggcg ttctccagca taaggagatg agaaagagac ttttccagaa
     3661 gagtggtttc cagaacatcg gccccggacc gacgcaacat agcgcgcgcg gccttccgcg
     3721 cttcaacgtt atctatcagg taatcgccag cttcgaattc aaagcgttca ccatcatcat
     3781 ccagggtgtc gcgttccagg cgatcacgta aggtccggtg ggcgcgggtg atcacgtcat
     3841 gatcatctga acgatcattt atgcgcttat tttggcgctt cgcattctcg actgcggcac
     3901 tgacaacggc attaactctt tgtttttccg ctatttcagc cgaaatgtga tcacctgcat
     3961 gttgatcatt agagtgatca atgatcatgc tttttagtgg cttcctgact ggcttatttg
     4021 gcttgcggct gtccgtagtc ctggtgtctt ctttgaaggc acggagataa cgacgtgcgg
     4081 tattagggtt aagattaaac tcggcggcat actgtgcgat ggtgtaacca ccatctcgcg
     4141 ccaggcgagc aaaattcttc ttgtgatcgt cccaggtcac ttatgcttcc tttcgtaaaa
     4201 actctttttg acgcgagggt aacgaaagtc acatgtcaaa aggcccggaa cgggcaagca
     4261 atcaatcaga tacgtgcgga tgtggcatta ccgtaatgac ggtgctgacg ggccacctta
     4321 ttgaaaagtt gacgcgccat tacccaaggc tggtgctccc ggcgttcctt ttcgtcctgc
     4381 gtcatataga gttcgttctg gagtttttca tcaaaccggc gcggagcgcg gctacggcga
     4441 aagaattcag gattcagaga gtggatctga aatctacgtg ggcgtgtact gtcatctatc
     4501 aaaacagacg aatacttaga cacagcgata gcctttaagc gcagataaac atcgcgctta
     4561 tcgacatcca gatgcgggta ttccttttca agaattgctg cgagttcttt cgctgataga
     4621 agagatttag tgcggatcat gtaatccgca atctcgtacg atgttattcg tgagtgattt
     4681 atttccatga agtggcgtcc ctgccagtta agtaacatcc tgtcacctac tgattagccc
     4741 atgtcaacta atcaacgtgg aatataatac cctcgattaa agaaatagca atacattaga
     4801 gcaattttat ctaacactcg acgaatgact tgtgataacg cccactccaa gcgcgtaatc
     4861 aaagaacaat cgttgatgca tcgccaacct accgtgcgtc ttctcccaat tatcgcggtc
     4921 acgctcaata tcacgctggc atgactggca cagaggaatt gcgtaaatgt catgcgcgca
     4981 taatcgacta tgacgaacga tataaggcgt aatgtgagcg ccagctcccg cagctccaca
     5041 cccacagcat ggacgggaag caacaaagtc catgtactca ggtaatttta gcgattgcag
     5101 ttttggtatt ttgaaatgcg ccatgccagg gtcggagtca acatccacag ggcatacttt
     5161 tgcacgcatc ggcgcggcgc gttcttccat catctgaaca tatgctgtag cgcgatcgtc
     5221 atacgggcga atatccgcct ctttcagagg tccgctatcc tgcggagtag ccttcatctt
     5281 atttattgat atgcggcaga cttcttccgg catcaggtgc atcatgttgc gcatgaaagc
     5341 ccaccagcac agctcctgaa tacttaaatc atggctattt gaaaggccca tttcctgacg
     5401 ggcgacatcc agtatccagt taacgcgatt attgtgcagc gtttctttca gctcattaaa
     5461 accacgcatc cggtaatggt tatcgtgatg ccagcacaac aacaccgcgc tattgtctcg
     5521 ttcagcgtgg acaatatggt tgtcacacca actacgatct gcggcctggc attgaccctc
     5581 tttcctacgc aaccacgcca ccagcgcgtc aattccacca atacggcgaa acagttcatc
     5641 gctgttaaaa aacggctgca acgcctcatt tgttgccatg gtttgctcgg taacaacgag
     5701 gccgtcttcc atgtgctcga ttaactcacg cggcaccggc tccataataa atttacggcc
     5761 agcctccacc agctttctga cctcctgatc cactttgaac gtggcgaggc caagctcttt
     5821 ctgtacaaat ggagtaatta cggctttcac atcacacctt taatcactga ttgggcttta
     5881 tctgctgccc ggcattctct gtttaagcac aaccatttcc tgacggcata acacagcaat
     5941 agctgtcctg gcaccaattt gcttaccaac caggtattgc tttaccttgc ggcgactcac
     6001 gccatcaaga agcatcttta acgcttcacg ggacaacttg ttgtatttgc gtgccattaa
     6061 tctactccgc agaaccatac aatctacgta acgtgtcggc gacagaagat acagatatct
     6121 cgccagtcgc agcgcctacg gtaaggtctg ccagttcagg tgaatcaaat acctgcaccc
     6181 cgttacggcg tagaaatagc agcgcactgt ttagcgcggt acgcttattg gcatcattga
     6241 atatatgccc tctcgctgta gccaccaggt aggtggcaga gacttcgaaa aggtcggtga
     6301 tctcttcgta ggcaactctg gcctgaactc tcccgataat ggcctctgcc ctacccggat
     6361 cagacattcc cggcaggccg ccgtagcggc ttatattcgc atcatgaagc gcaataagtt
     6421 cttccggtga tatatgcctc attatcggtt aaccagttcc ttgttggtgg agtccagggt
     6481 gtcaaacagg gatgcaaatt cagcatccag cgccgctttt ttgtaggctt cgaaagtagc
     6541 cttgctgaca attactgctg gctcacggcc tctgcgggtg atttcaacct cttccccggc
     6601 ttcaacattg ttgagcactt cagaaaggtt gccacgcgcg gtacggaagt taatggattg
     6661 cataaacacc tcgtgtactc gttatgtgta cacaattata aacttcacag gcataaagca
     6721 ccagcccttt gcagcttaaa taaccggaca atcatcaaac tccccacttc tggcatcatt
     6781 gatgacatga gtgatcacac caaaaacagc attactgccc gtgtatccat cgtcatctac
     6841 tggtaacgcc tctttcttcc cggtgcttaa atcctccagg tgctggcgcg gatacttcct
     6901 gtatctcttt atgcgatatt caccctccat agcgcacaca agcagagaac catcaaccgg
     6961 agtaagcgag gaatcaacca ccagcaaagc accctgcaat attccctcac ggtgatggct
     7021 atcagctgcc cgcatgaagt aggttgctga tggatgcctg attagttgct gatcaagaga
     7081 aattcggctt tcaacataat ccgccgcagg agaagggaag cccatagcgt tttcacctca
     7141 ataatactgt tcatttatac agtatacatt aaagagacac ctttggtgca aacgcgttac
     7201 gtacatcaac caccgctgat gattttgtgc tctttgctac tattcatcac caacggatca
     7261 gcgtaacctc gttgccaatc agttaataag gaattagcta tgcctgcccg cattcctctc
     7321 gatcctgtat tgcccaaaaa ttttgactgc actcctaacg agaaacgctc taaagctcag
     7381 ctggacgcct ggtgggacca tccatatggg gttacacaac ctgacgggaa aattgtagtt
     7441 tattgtctga atggtggggc gtgggaccgt ccatccgtgc tttgtttggc agataactat
     7501 gatgaagcct gtgaacttgc cgaaagacag caggcaagct gggtaaaaac acggtctgaa
     7561 ccgacattca tgttttcaaa agaaccgcca tttatactgg cgaggatgcc gcagcgaccg
     7621 gatcatcaac aagaaattgt tgctgaattt tcctcaaggg atgagataaa tctcttctca
     7681 ttaaagcagg aagaaaggga tcgcgtcgaa gtgtctccaa ctctcgacca caaccggatg
     7741 aacctggccc agctcgcctg gtacagcaaa gaattagaga tgtctattgc ccggcttgaa
     7801 aacgaaaaag ccgctatcca agtccagcat gaagtagttc tgaaccggat tagagaaatg
     7861 caaaacgata acaggggatt ttgaatggct aaaatcgagt accatcgcga tcgcggtaat
     7921 tacctggaaa tatacgatca tgaatctctt aacgatatca acgatgcgtt atatgaatac
     7981 tgtgaaaaaa cgagcatcac agatgcacct gatgcatttg tcgagctacc ggtatatctc
     8041 cgcgacatct atgcaatacg aacaccgccc gtatcggtaa ttcacattgg ctatgtccgc
     8101 ctgtccatcg aagaagatga agatcgttat atcgtgcgcc actatacatt ggacagaaaa
     8161 gagcttccca atgaatggaa catgagtaat ttctacaacg gtgaatatgg cttaaaaccc
     8221 gctaagaatg gcgcgggcat tgcgtaatag cctaacaact attctgtttt ctgatatgcc
     8281 ggatcgttcc cttttggcaa ctggaggctt aactgccgat agtgccgtaa ccgttccatg
     8341 aaataggtgc gcagattctc tggttgctcg cgggctacct gttcagctat gacaggtatg
     8401 ttcaatcgct ctttgtacgc cacaccgctg gcagccagat caacgttaac cttatcccgt
     8461 tcttcctgac ttttagctgc aatattccag tcgctcatat ttaaggctca catttccaga
     8521 tggtattctg aacacccgaa ccgggcgcaa gattagggtt agcgttcgcg ctatgctgat
     8581 atactgcttt agacttccca tattgctgac aggctttatc tgcggttttt tgcaggctat
     8641 ccaggccata ccaaccatct gactgtatgc ttaccttttc accgtcgttg tattgcacca
     8701 ttgcacaccc agatatagcc agtatcgcgc cgacaataac gcttttccat aaaactctat
     8761 gcaacatata caaaaatccc ctctgtgaat tgaggggatt ttagcatggt gatcagatat
     8821 cggccttatt cggaatttta tcagccacca gcggcaataa agccttcgcc atttcatgaa
     8881 ccaacatggc atcaatgacg cctagcgtat ggcccggctt aatctttaat gcggcctcaa
     8941 gatagcctct ttccagagtg gtttttacgc ttttcttggg cgattgttgg gaactatccg
     9001 gaaaatccgg atggttgcca gccacataag ctacccgcaa ccagtgcatg aatgtttccg
     9061 cagacacaca accgcagtcc acatcgattt tcccgcgttg ctgttccagc cattgctcaa
     9121 aatctaactt gtaagcctta ctttcaggtt catcaccatt gaactcgact ttctgcgacg
     9181 ctatgagagc agcttcgtat tgttcgcgag tgacaactga ctggtattca tcgctatcaa
     9241 ggtcaccgat tggaagctca atctcacaac aaaaattgcg cccaaagaaa gtgtcttttt
     9301 tgtggtccga gccaaaagca aaagtcgcgc atggcgccat taaattggca ctgagcaggc
     9361 gacaatcgat cataccatca ggccacccgc cgcacttggg cagctccttc actaacaagt
     9421 cgataagctt cattttttta tcatctttgc aagccgccaa agccatttgg gcaagtgcca
     9481 atacttcatc tgccgtatat ccagcaccgt gaccatacat ttcgatacgg gcgataatct
     9541 ctgatatgcg ttctttagtg attcttgtca tttctttttg cgccatttct tttcacattc
     9601 cttagtccat ttttcaatgt tcattttggc aatatcagtc attccatcac ctaagaaata
     9661 ctttttccgg tatgccttgc acttaaacca cactacaaca gccaccagcc agaaaataaa
     9721 aggccataca gcaataccaa cgacagccgc gataaagccc aatagccata aatgaagctc
     9781 tccaacttct gtttccggca atattcttaa agaattaaac agcaggctga acgaatggtc
     9841 gtatgcattg gcagtataag acatgcaatc catataatta aagtcatagc ctgcggctgc
     9901 cgcccataat gggcggtcaa gaaaatgttt tagtgtcatc atataaattt aaggttcaga
     9961 ccagttatct tcaatagcaa tgcttaatct ttgtagccat tctgctaatt tcagcattgc
    10021 ttctctttcg cttaaaccac gaggaaaatc atcaagtgaa attgttggct tgaatccccc
    10081 gtaattatcc atttcaactg tcagattttg ttccagcacg gtatttctta cgcgactatt
    10141 gtgccggagc aaatatactg agcgtgattt attggttttg tggtctagct tatattcggt
    10201 aagtatcatc tggcttttgc catgattatt acctctccac atacttacct cacttaataa
    10261 aacaactcca tgcgtagttg atgatttttt cccacgtaat ataaatctgc actccggcag
    10321 taaaaccaaa gccaacaatt gctgaaaaaa tcaaaacatt tacttttgac attataaatt
    10381 ttctctcggt gtcgtaggtt atagcaccat aattgataat ttagtgagtt agcagttcca
    10441 ttttttggat gatttccgca tgagcatcat cgttgtcaac acttaactcg tttaatgcct
    10501 ctcgcaccac atcaacttct tctggttgga agaagtcatc tcggtagtca ccaaatagaa
    10561 ccgaaacaag ccttccacca gcaacatcaa gattggcgct aacaggtggc tctttaccat
    10621 cctcaaattc gactacaaaa gttaatttcc ccatcgttac caccagcgac aaattgaata
    10681 caaacccagt gctgccgcca tcacaattcc taccgttgcg aatgcttcag gccagctcat
    10741 tgacctgcct cctggggagg ttctggtagc agcatccaga acaaggcgtt ccctaaccag
    10801 gataaagtgc cgtcgctcaa ctccacgtat tccccttgca cctgtcctgc catatactca
    10861 ccgtgctttg aataaattaa aatccaatca tcttgagcgg gcattcgctc actacagctt
    10921 atccaaccat ccggagttac ctgagaattg ccatttacca agtcagctcg aacatatagc
    10981 gtgtcatcat ggtgctgatt gtggctgcac cacgttaatt cgcttaactc gccatcttct
    11041 ggccatactc cagctgtttg cagccagata tgggctggcg catcctggca aggtgtatta
    11101 actggaaact tgtaagtttg gcttacaggt tggctaccct gaagcatggc agcgtggcag
    11161 gcatactcaa cgcccttaac tgcatctgcg cagtagttat agcgattgca ttccactaat
    11221 ttccgtttga gtttttcaat tgcctgcgcg acatcagcct gtattacagg aactggcgga
    11281 acggctgttt gctctcgaac gtcattagtc gctatcggtt ctgctgccaa ctgactggca
    11341 tatttgttaa tggtaacgat aagctcttgc tcagcctcat ccagacaatc accgatacct
    11401 cgcctgtcac cgtcaaaatc atcgaaatcg gcacgaatct tggcaacctt caggattgcg
    11461 gacaacactt cactaggaat taccggatag ttggttgacg tttccgcgat ttcccgaaaa
    11521 ttattggttg acgaattctt attttcccga aagtttccgg actgaagcat ggcggcgcgg
    11581 caggcgttcc agcctctcac ctctgcaata gcggcaacag catcaaccgc gtacatgcta
    11641 agaggattag gcattggttt ttcttccggt actactggaa cggggggagc ggcgtagacc
    11701 tcaataattc cattatcaat aggccattct ccatccttga ggtagtcact tgtgccgtca
    11761 acttgctgtt ctgcaatgtg gaatgcacct attggttttg cttccagcga tgccagagca
    11821 atccgtgcca gttcttccgc ttcttctgct ggcagtacaa cgttgctacc cggtccgtat
    11881 gtttcgcgcc actgcttgat tgtcagcagt cgctctttgg ttatagtggt catttgttaa
    11941 tcctcaaaac tttatgcccg ggcgcaaaag cacgcgtttt gtctttgctt attcgccagc
    12001 catccttgcg cgcctctttt gcacagccag cccatgacgt acctatatac tcaccgaagt
    12061 ctggcgactt atatttgcca tctgtacact ggaggcaatc acaatagaga tgcatggtgt
    12121 aacttgcagc gatagccata tcactctcct ttagtgcgca agtggttttt ccagcggttt
    12181 tgcgccgcgc tgggcttttt gcaaaaacca caatccatca tcccgtaata tttcatcaac
    12241 cccatccgtc ggttgctgag tctcacccac tgccagacgc caggagcgtt tctacgaact
    12301 aacagaatct ttgctttacg gtttttcatc ttacagcgta ccctttcttc cgcctgttct
    12361 gtgacgcagt aggcttacgc tttgcggcaa aagccacctg accaaatgga tggagtaccg
    12421 ctatcttatg gttgctaata accagctcca ccacacgcac aggtcgctgt aaaaaaagtc
    12481 gttttgcctt acggtttttc atcgctttgc tctcctgcgt ctctttgctg ctcgtcgtgc
    12541 cgctgcaata ccggtatggc ggcgctttgg tgccgggatg atgttgtcag ccatcaggac
    12601 atacggcttt gcaattagcg cagaagccca aaaacgagtc gggtacggta acaagccgat
    12661 acatgccaca cgcattactc acctcctttg atgcgaatgc ctgcggcgcg gattgcatcg
    12721 atgacttcag aaactttgta tgccattacc gtttggtaat catcgtgaaa atctgttcga
    12781 tgaagcatgc tgctacgttc cgggagcgat atttcccgag catccagttc cttaacgcgt
    12841 tcctccagtt cgtagaccct gcattgttct ctatcatcaa tcagatataa cccaagacat
    12901 tcgctttcta cccaaccgcc aaaatcatga tcgtaacgct cacatgaaaa ctcaccgtca
    12961 ccgtcctttg ttggaatggt gtaactatct aatgggccac catatgtcgg cacatttccc
    13021 aatgttggat gctcaatcca catgaaaaat gcacgtccgg ttattgggca aatatctggc
    13081 cgccattggt tacgaacagc cttggtttcg gataattctt cagcgtgttg ttttacttcc
    13141 tcaagctcaa ctctcagctt ccctaccgtt agcgcaatat cctcgttctc ctggtcgcgg
    13201 cattttatgt attgctggtt tctttcccgt tcatccagta gtgccagcac ggtttctggt
    13261 ccggtcagaa atttgaaggc gttgagcgca tcaatatcca caccgtaatc cttaagttcc
    13321 tgttcagtta acaaatcatc atcaactggc aacattaaca ggtgttccat tgccggaatt
    13381 gcacgctctg ccgcctcacg cagtgcctga tagtcaattg tcattctcgc catccttcac
    13441 agttgtaatc actacagcct tcaaaatcat atgggctgta ctgccaggtt atttttccgc
    13501 aatgcggaca attccaacgc accttcccgc ttcgcgactt ctttcttctg ttctgctctt
    13561 tcaaccagtc aggcatgacc aaacctgcgc cctgaaccat tgttctgcgg ttaaagttat
    13621 tgatattgaa cgtccgacgc tttgctgcat cagcaatgga aaatggcaac caaactattc
    13681 ctggttcgtt tttgttggcg acgctaaaga tggtcgcttt actgaagtca tctgttggca
    13741 atccaccgtg ttgaagccag taaacatcgt tgccgttcca gctacctttt ttgtaggcca
    13801 catacgcagt gcaatctgac tcaatcaggc tttctgtggg gatgtactgg caatcaacgt
    13861 gccacacagc cattgcatcc acgctatcag cgcaaacagg ctgatcgata tctcgtccac
    13921 aattccaggc tttttgggct tcttccagcg tgtaaacatg agcgcgatcg atatcagaac
    13981 tgtaaccatt gccgttatgg caatggaatg aggcgttatt acccacagtt tcacgcaagc
    14041 acatcatgta aaagcggtta ttcactggtt gcctccgctt cccacgtttt cagactttca
    14101 ccacagaacg ggcaaaatga aactcgaata ggcgatttag aaaattcacc agaccgcagc
    14161 atgatcaggt cttgtgaatg aattaattca tggttataga ttttgtattt cagcagacct
    14221 tttcgcgtcg tgtattcagc gtcatgctcc agggattgtg ccaacgccgc gcacggttct
    14281 atcttgttgc cattaatttg gcattttgac tcactcactg gttgccccct gaatacgctc
    14341 aaactctatt acccacaccc aaggattagc gttccaacta tcttcgccat aaattgattt
    14401 ccataggcta cggaaacctg ggtaatgctt atcgccaatg agggtcgatt ctgttggtgc
    14461 gccctcagcc cttgcatcgc attcgctgat atcgttcaac cgctcaacgc gcacgttggt
    14521 aatttccaga agaatgcgcg atgcccagcg cggcatgtga attgatggcg tccacttttc
    14581 tgatactggt ttattacaaa cctcgaccgg aacccggtgc gtttgttctg tccaggagtt
    14641 acgcacgctt gcgcgataaa ccagcgttgc gacgtccgtc gctttgccat gtacccggta
    14701 ggtttcgcga acccaaatac gatcgcccgg ttgaccatat ggacaatgct tggcaagcaa
    14761 ctctgcggcc actgcccgtc catagaattt ttcttcaaca atcctgcgag tctgtgtttt
    14821 attcccgcca agaattgccc ggaccatctc atcgttaaaa atcatgccgc gctctttcac
    14881 ttcgcctttc atgcatcccc cttacccatg tgcgacgatg ccgccaaaag tgatagagaa
    14941 cagccagaaa tagatcgcgg ccataatgat tttgaatgcc gtgttcatat tttcagctcc
    15001 tgtgattgat tggatacatg ccgcgcc
//
LOCUS       DAPGOF010000113        15020 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.114, whole genome shotgun sequence.
ACCESSION   DAPGOF010000113 DAPGOF010000000
VERSION     DAPGOF010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15020
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.114"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            122..211
                     /gene="kdpF"
                     /locus_tag="RHV13_003152"
     CDS             122..211
                     /gene="kdpF"
                     /locus_tag="RHV13_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HDV1242664.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            211..1884
                     /gene="kdpA"
                     /locus_tag="RHV13_003153"
     CDS             211..1884
                     /gene="kdpA"
                     /locus_tag="RHV13_003153"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HDV1242665.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALVIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            1907..3955
                     /gene="kdpB"
                     /locus_tag="RHV13_003154"
     CDS             1907..3955
                     /gene="kdpB"
                     /locus_tag="RHV13_003154"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HDV1242666.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWITVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRL
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAVTYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            3964..4536
                     /gene="kdpC"
                     /locus_tag="RHV13_003155"
     CDS             3964..4536
                     /gene="kdpC"
                     /locus_tag="RHV13_003155"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HDV1242667.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASASVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            4529..7213
                     /gene="kdpD"
                     /locus_tag="RHV13_003156"
     CDS             4529..7213
                     /gene="kdpD"
                     /locus_tag="RHV13_003156"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HDV1242668.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            7210..7887
                     /gene="kdpE"
                     /locus_tag="RHV13_003157"
     CDS             7210..7887
                     /gene="kdpE"
                     /locus_tag="RHV13_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HDV1242669.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            complement(8051..9691)
                     /gene="pgm"
                     /locus_tag="RHV13_003158"
     CDS             complement(8051..9691)
                     /gene="pgm"
                     /locus_tag="RHV13_003158"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HDV1242670.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALLADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(9717..10262)
                     /gene="seqA"
                     /locus_tag="RHV13_003159"
     CDS             complement(9717..10262)
                     /gene="seqA"
                     /locus_tag="RHV13_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HDV1242671.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            10447..11211
                     /gene="ybfF"
                     /locus_tag="RHV13_003160"
     CDS             10447..11211
                     /gene="ybfF"
                     /locus_tag="RHV13_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDV1242672.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPTLFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            11351..11644
                     /gene="ybfE"
                     /locus_tag="RHV13_003161"
     CDS             11351..11644
                     /gene="ybfE"
                     /locus_tag="RHV13_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HDV1242673.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            11784..12314
                     /gene="fldA"
                     /locus_tag="RHV13_003162"
     CDS             11784..12314
                     /gene="fldA"
                     /locus_tag="RHV13_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HDV1242674.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            12524..12610
                     /gene="uof"
                     /locus_tag="RHV13_003163"
     CDS             12524..12610
                     /gene="uof"
                     /locus_tag="RHV13_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HDV1242675.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            12603..13049
                     /gene="fur"
                     /locus_tag="RHV13_003164"
     CDS             12603..13049
                     /gene="fur"
                     /locus_tag="RHV13_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HDV1242676.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(13133..13459)
                     /locus_tag="RHV13_003165"
     CDS             complement(13133..13459)
                     /locus_tag="RHV13_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242677.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(13509..14915)
                     /gene="chiP"
                     /locus_tag="RHV13_003166"
     CDS             complement(13509..14915)
                     /gene="chiP"
                     /locus_tag="RHV13_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HDV1242678.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFVLEAAFGQAGGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         3348 a         3898 c         4127 g         3647 t
ORIGIN      
        1 ttaaatcatg gcttttgcca tttttatact ttttttacac cccgtccgca gatttttgcg
       61 aaatctttgc agccagaatt ctacccttcc ggtatcactt ttaggccact ggaggtgcac
      121 tgtgagtgca ggcgtgataa ccggcgtatt gctggtgttt ttattactgg gttatctggt
      181 ttatgccctg atcaatgcgg aggcgttctg atggctgcgc aagggttctt actgatcgcc
      241 acgtttttac tggtgttaat ggtgctggcg cgtcctttag gcagcgggct ggcgcggctg
      301 attaatgaca ttcctcttcc aggtacagcg ggcgttgaac gtatactctt tcgtttacct
      361 ggcgtctctg accatgagat gaactggaag cagtatctct gtgccattct cggcctgaac
      421 atgctggggc tggcggtgct gttttttatg ttgctcggtc agcactatct gccgcttaat
      481 ccacagcagt tgccggggct gtcgtgggat ctggcgctga ataccgccgt cagctttgtc
      541 acgaatacca actggcaatc ttatagcggt gaaaccacgt tgagctattt cagccagatg
      601 gcaggcttaa cggtacaaaa ctttctttct gccgccagcg ggattgcggt gatttttgcc
      661 ctcattcgtg catttacccg ccagagcatg agcacactcg ggaatgcctg ggtcgatctg
      721 ctacgcatca cgttatgggt gctcgtccct gtggcgttgg tgatcgcact gttttttatt
      781 caacaaggtg cgctgcaaaa ctttctgccc tatcaggcag tgaataccgt tgaaggagcg
      841 caacagctgt taccaatggg tccggtggct tctcaggaag cgatcaagat gctcggcact
      901 aacggcggtg gtttctttaa tgccaactcg tcgcatccgt ttgaaaaccc aaccgcactg
      961 accaacttcg tgcagatgct ggcgatcttc ttgatcccaa cagcgctgtg ctttgccttt
     1021 ggtgaagtga cgggcgatcg ccgccagggg cgcatgttgc tgtgggcgat gtcggtgatt
     1081 tttgtcatct gcgtaggcgt ggtgatgtgg gcagaagttc agggtaatcc tcatctgctg
     1141 gcgctgggtg cggacagcag catcaatatg gaaggtaaag agagccgttt cggcgtgttg
     1201 gtcagtagcc tgtttgcggt cgtgactacg gcggcgtcct gtggcgcggt gattgcgatg
     1261 catgattcgt ttaccgctct cggtggcatg gtgccgatgt ggctgatgca aattggtgaa
     1321 gtggtattcg gcggtgtcgg ttctggtctt tacggcatga tgctgtttgt cctgctggcg
     1381 gtgtttattg ccgggctgat gattggtcgt acaccggaat atctgggtaa aaaaatcgac
     1441 gtacgtgaga tgaaactgac tgcactggcg attctggtca ccccgacgct ggtgctgatg
     1501 ggcgcggcgc tggcaatgat gaccgacgcc ggacgtagcg ccatgctcaa ccctggcccg
     1561 catggtttta gcgaagtgct gtacgccgtg tcatccgccg ctaacaacaa cggcagcgcc
     1621 tttgccggat taagcgccaa ctctccgttc tggaactgtt tactggcgtt ctgcatgttt
     1681 gtcggtcgct tcggggtgat tatcccggtg atggcaattg ccggttcgct ggtgagtaaa
     1741 aagagccaac ccgccagctc cggcacgctg ccaacgcacg gcccgctgtt tgttggcctg
     1801 ttaatcggca ccgtgttgct ggttggcgca ctgaccttta tccctgccct ggcgcttggt
     1861 ccggtggcgg aatatctctc ctgatgatat tgagtgagca ctgaatatga gtcgtaaaca
     1921 actggcgcta ttcgaaccaa cacttgtcgt tcaggcgctg aaagaagcgg tgaaaaaatt
     1981 aaacccgcag gcgcaatggc gcaatccggt gatgtttatc gtctggatcg gcagtctgct
     2041 gaccacctgt attagcatcg cgatggcaag cggtgcgatg cccggcaatg cgctgtttag
     2101 cgcggccatt agcggttggc tgtggatcac cgtactgttc gctaatttcg ccgaggcgct
     2161 ggcagaaggc cgcagtaaag cgcaggccaa cagtctgaaa ggggtgaaaa aaactgcctt
     2221 tgcccgcaag ctgcgtgagc cgaaatatgg cgctgcggcg gacaaagttc ctgccgatca
     2281 acttcgtaaa ggcgatatcg tactggtaga agctggcgat attatcccct gcgatggtga
     2341 agttattgaa gggggtgcat cggtcgatga aagcgccatc accggggaat cggcaccggt
     2401 gatccgtgaa tccggcggcg attttgcctc cgtcactggc ggcacgcgta ttctttctga
     2461 ctggctggtg attgagtgta gcgttaaccc cggcgagaca tttctggatc ggatgatcgc
     2521 gatggtggaa ggcgcacagc gacgtaaaac gccgaacgag attgccctga ccatcctgct
     2581 gattgccctg actatcgtct ttttactggc aaccgccacg ctgtggccgt tttccgcgtg
     2641 gggcggtaat gcagtcagtg taacggtact ggtggcgctg ctggtctgtc tgatcccaac
     2701 cactattggc ggcctgttgt cagcgatcgg cgtcgccggg atgagtcgga tgctgggcgc
     2761 gaatgtgatt gccaccagcg gacgtgcagt tgaagcggca ggtgacgttg acgttctgct
     2821 gctggataaa accggcacca tcacactcgg taaccgtcag gcatcggagt ttatccccgc
     2881 gcagggcgtg gatgaaaaaa cactggctga cgccgcacaa ctggcttcgc tggctgatga
     2941 aacgccggaa ggccgcagta ttgtgatcct cgccaagcaa cgttttaacc tgcgcgagcg
     3001 cgatgtgcag tcgctccatg ccacctttgt accgtttact gcgcaaagcc ggatgagcgg
     3061 gataaacatc gacaaccgct tgatccgtaa aggttctgtc gatgccattc gccgccatgt
     3121 tgaggctaac ggtggtcact tccctgccga tgttgatcaa aaagtcgatc aggttgcgcg
     3181 tcagggagcc acgccgctgg tggtggtgga aggttctcgt gtgctgggtg ttattgcgct
     3241 gaaagatatc gtcaaaggcg gtattaaaga gcgcttcgcc cagctgcgta aaatgggcat
     3301 taaaacggtg atgattaccg gcgataaccg tctgactgcc gccgcgattg ctgcggaagc
     3361 gggtgtcgat gattttctcg ccgaagcgac accggaggcc aagctggcat tgatccgtca
     3421 gtatcaggcg gaaggccgtt tggtagcgat gaccggcgac ggcaccaacg atgctccggc
     3481 gctggcgcag gcagatgtcg cggtggcgat gaactctggt actcaggcgg cgaaagaagc
     3541 gggcaatatg gtggatctcg actctaaccc gaccaagttg atcgaggtgg tgcatattgg
     3601 caaacagatg ctgatgaccc gtggctcgct gaccaccttc agcattgcca acgatgtggc
     3661 gaaatacttc gccattattc cggcggcatt cgcggtaacg tatccacagt taaatgcgct
     3721 gaacatcatg cgcctgcatt cgcccgactc cgccattctc agtgcggtca ttttcaacgc
     3781 cttgattatc gtctttttga ttcccctggc gttaaaaggc gtgagttata aaccgcttac
     3841 cgcttctgcc atgttgcgcc gtaacttatg gatttacggt ctgggtgggc tgctggtgcc
     3901 gtttattggt atcaaagtca ttgatttact gctgaccgtt tgcggtctgg tgtgaggttt
     3961 accatgcgtg gattacgtcc ggcattatcg acatttatct ttctgttatt gattactggc
     4021 ggtgtttacc cgctgctgac caccgcgctg gggcaatggt ggtttccctg gcaggccaac
     4081 ggttcgttga tccgtgaagg tgatacggtg cggggttcgg cattaatcgg gcagaatttt
     4141 accggcaacg gctattttca tggtcgcccg tcggcaacgg cagaaatgcc ttataatcca
     4201 caggcttctg gcgggagcaa tctggcagtc agtaacccgg agctggataa acagatagcc
     4261 gcacgcgttg ctgcattacg ggccgctaac ccggatgcca gcgcgagcgt tccggttgaa
     4321 ctggtgacgg catcggcaag cgggctggac aataacatca ccccacaagc ggcggcctgg
     4381 cagatcccac gcgtagcgaa agcgcgtaat ctcagcgttg aacagctcac gcaactgatc
     4441 gcaaaataca gccaacaacc actggtgaaa tatatcggcc agccggtcgt caacattgtc
     4501 gaactcaatc tggcgctgga taaacttgat gaataacgaa cccttacgtc ccgaccccga
     4561 tcgtctgctg gaacaaactg ccgcgccgca tcgggggaag ctgaaagttt tcttcggtgc
     4621 ctgcgcaggc gtcgggaaga cctgggcgat gctggcagaa gcccagcgac tgcgggcgca
     4681 agggctggat attgtggttg gcgtggtaga aacccacggg cggaaagata ccgccgccat
     4741 gctggaaggg ctggctgttc tgccgccaaa acgcctggcg taccgtgggc ggcacatcag
     4801 cgagtttgat ctcgatgccg ccctcgcccg ccgtccggcg ctgatcttaa tggacgaact
     4861 ggcgcacagt aatgcgccag gttctcgtca tcctaaacgc tggcaggata tcgaagaact
     4921 gctggaagct ggcattgatg ttttcacaac cgtcaacgtt cagcatctgg aaagtctgaa
     4981 tgatgtggtc agcggcgtca ccggaattca ggtacgggaa accgtgcccg atcctttttt
     5041 cgatgccgcc gacgacgtgg tgctggtgga cttgcccccg gacgatctgc gccagcggct
     5101 gaaagaaggc aaagtctata ttgctgggca ggcggagcgc gccattgaac attttttccg
     5161 caaaggcaat ctgatcgccc tgcgcgaact ggcactgcgc cgtactgccg atcgcgttga
     5221 tgagcaaatg cgcgcctggc gggggcatcc tggcgaagag aaagtctggc acacgcgcga
     5281 cgcgatcctt ttatgcatcg gccataacac cggcagcgaa aaactggtcc gcgcggcggc
     5341 gcggctggcg tcacggctgg ggagcgtctg gcacgcggtg tatgttgaaa cccctgccct
     5401 gcatcgctta ccggagaaaa aacgtcgggc aattctcagc gccttacgtc tggcgcagga
     5461 gctgggcgcg gagacggcaa cactttctga tccagcggaa gagaaagcgg tagtgcgtta
     5521 tgcccgtgaa cataatctcg gcaagattat tctcggtcgc ccagcctcgc gccgctggtg
     5581 gcgtcgggaa acgtttgctg accgactggc gcgcatcgcc cccgatctcg atcaggtgct
     5641 ggtcgcgctt gatgaaccac ccgcccgcac gattaacaac gcgccggata gccgctcttt
     5701 taaagacaag tggcgggtac agattcaggg atgcgtggtt gccgccgcgt tatgcgccgt
     5761 tatcacctta attgccatgc agtggctgat ggcgtttgat gccgccaacc tggtgatgct
     5821 gtatctactt ggcgtggtgg tggtggcgct attttatgga cgctggcctt cggtggttgc
     5881 taccgtcatt aatgtggtga gtttcgatct cttttttatc gccccacgcg gcacgctcgc
     5941 cgtctctgat gtgcaatatc tgctgacctt cgcggtgatg ttaaccgtcg ggctggtgat
     6001 cgggaacctt actgcgggtg tgcgttatca ggcgcgggta gcccgttacc gcgagcaacg
     6061 cacacggcac ttatatgaaa tgtcgaaagc tctggctgtg ggccgcagtc cgcaggacat
     6121 cgccgccacc agcgaacaat ttattgcctc cacgtttcat gcccgcagcc aggtattgtt
     6181 acccgatgac aacggtaaat tgcagccgtt aacgcatccg caaggaatga cgccgtggga
     6241 cgatgccatc gcgcagtgga gttttgataa aggcctgcct gcgggtgcgg gcaccgacac
     6301 gttacccggc gtgccatatc agattttgcc gttaaaaagc ggtgagaaaa cttacgggct
     6361 ggtggtggtg gaaccgggga atctgcgcca gttgatgatc ccggaacagc agcgcctgct
     6421 ggagacgttt acgctgttag tcgccaatgc gcttgagcgg ctgacgctga ccgccagcga
     6481 agaacaggcg cggatggcaa gcgaacgtga acagatccgc aacgccctgc tggcagcgct
     6541 ttcgcatgat ttacgcactc cgctcacggt gttgttcggt caggcagaaa tcttaacgct
     6601 cgatctggca agcgaaggat cgccccacgc ccgccaggcc agcgagattc gtcagcatgt
     6661 gctgaacact acccgactgg tgaataatct actggatatg gcgcgaattc agtccggcgg
     6721 ctttaatttg aagaaagagt ggttaacgct ggaagaagta gtcggcagcg cgctgcaaat
     6781 gctggaaccg ggtttatcgt cgcccatcaa tctttctctg ccagaaccgc tgaccttaat
     6841 ccacgttgac gggccactct ttgaacgggt gctgattaat ctgctggaga acgcggtgaa
     6901 atatgcgggt gcgcaggccg aaattggtat cgatgcccac gttgagggcg aaaatctaca
     6961 actggatgtc tgggataacg gccccggtct tccgccaggc caggagcaga cgatatttga
     7021 taagtttgct cgcgggaata aagagtcggc agtaccgggg gtagggcttg gactggcaat
     7081 ttgtcgggcg attgtggatg tacacggcgg caccattacc gcgttcaacc gaccggaagg
     7141 tggtgcctgt tttcgtgtta cacttcccca gcaaaccgct cctgaacttg aagaatttca
     7201 tgaggatatg tgacaaacgt tctgattgtt gaagatgaac aagctattcg tcgctttctg
     7261 cgcacggcgc tggagggcga cgggatgcgc gtctatgagg ccgaaacgct gcaacgcggc
     7321 ttgctggaag cggctacccg taagccagat ttgattattc tcgatctcgg cctgcccgat
     7381 ggtgatggga ttgagtttat ccgcgacctg cgccagtgga gcgcggtgcc ggtgattgtg
     7441 ctttccgcac gcagcgaaga gagcgacaaa atcgccgcgc tggatgccgg agctgatgat
     7501 tacctgagta agccgtttgg cattggcgaa ttgcaggccc gtctgcgcgt cgccttacgc
     7561 cgccattcag ccaccgccgc acccgatccg ctggtgaaat tttccgatgt gaccgtcgat
     7621 ttagccgccc gcgtgattca ccggggtgag gaagaggtgc atcttacgcc aattgaattt
     7681 cgcctgctgg cggtgctgct caacaatgcc ggaaaagtgc tcacccagcg ccaactcctt
     7741 aaccaggtat gggggccaaa cgcggtcgaa cacagtcact atttgcgtat ttatatggga
     7801 catctgcgac aaaaactgga acaggatccc gcccgccccc gccatttcat tactgaaacc
     7861 ggtattggtt atcggtttat gctttaggcg gttaaatgct ggatccggcg tgaacgcctt
     7921 atccggccta caaaatagct taaattcaat aaattgcacc atcctcgcag gcctgataag
     7981 catagcgcat caggcagtca taagtaaaaa agggcgatct tgcgaccgcc ctttttctta
     8041 tttaatgtgt ttacgcgttt ttcagaactt cgctaacaat ctcaaccgct tctttctcaa
     8101 tctgcttgcg atgttcttca ccgaggaagc tttcgcagta gatcttatat gcgtcttccg
     8161 tgcctgacgg acgcgcggcg aaccagccgt tgtcagtcat cactttcaga ccgccaatag
     8221 aagcaccgtt gcccggagca gcagtcaggc gcgcggtgat cgggtcacca gccagggtgc
     8281 tggcgctcac catttccgga gacagcttag acagcgccgc tttttgtgcg gaagtcgcag
     8341 ctgcctgcaa acggttgtag ctcggcgcac caaagcgttt tgccagttcg ttgtagtgtt
     8401 cctgcgggtt cttaccggtg acagcggtga tttccgccgc cagcagacac atgatgatgc
     8461 cgtctttgtc ggtggaccac ggcgtgccgt cgaaacgcag gaaggaagcc cctgcgctct
     8521 cttcgccgcc aaagccgaag ctgccgtcga acagaccatc aacaaaccat ttgaaaccta
     8581 ccgggacttc taccagctta cgacccaagt cattgaccac acggtcgatc atcgcagaag
     8641 aaaccagcgt tttaccaacg gcaacatctt tgccccactg cggacgatgc tggaacaggt
     8701 aattgatcgc caccgccagg tagtggttcg gattcatcaa acctgccgga gtgacgatac
     8761 cgtgacggtc ataatccggg tcgttagcaa acgccagatc gaacttatca cgcagtgcca
     8821 gcaggcccgc catcgcacac tcggaggagc agtccatacg gatcgcgccg tctttatcaa
     8881 ggtgcataaa gcggaaggtt tgatcgacct gatcgttaac gatagtcagg ttgaggttgt
     8941 aatactcgcc aatacgcttc cagtattcga taccggaacc gcccagcgga tcaacgccaa
     9001 gcgtcaggcc cgctttctga atcgccgcca tatcaacgat atcggccagc ccttccacga
     9061 acggctgcac cagatcctgc tctttcacat gaccggatgc catcgcttcg tcgagggaga
     9121 tacgcttcac gcctttcagg ccatcggcca gcagtgcgtt ggcacgatct tccaccactt
     9181 tagtgacgtt ggtatcagcc gggccaccat ttggcggatt gtacttgata ccaccatctt
     9241 ccggcgggtt atgggacggt gtaataacga taccgtctgc cagcgggcca ccttttttat
     9301 tgtgaaccag gatggcattg gaaacggcag gcgtcggggt gaagccattg ttttcctgca
     9361 caataacatc tacgccgttc gctgccagca cttccagaac ggaaataaat gcaggttcgg
     9421 acagggcgtg agtatcttta cccacatagc aagggccagt gatgccgttt ttcgcacgtt
     9481 cttcagcaat tgcctgagcg atcgccagaa tgtgcggctc gttaaagctg tggcgcgctg
     9541 cactgccacg gtgaccggaa gtaccgaatt tcaccgcgtg ctccgcattc cctgcttctg
     9601 gtttcagtac ataatattgc gccgtcagtt gggcgacgtt aatcaaatca ctctgttgtg
     9661 caggttggcc tgcacgattg tggattgcca ttgctttgtc ctttgtctgc aacgtgttag
     9721 atagttccgc aaaccttctc aatcaattcc gccgggaatt gcatcgactg catgatgtgt
     9781 tcgatcatgc tgcatttacg gccggtgttg gtgttggtga tcacccaata cggcgtgcct
     9841 ggcacatgtt tcggcttggt ctgattacca tttttcagca gcgtttgttc atctgccgca
     9901 aagtaaacgc gcgtacgacc gtgcaacgat tccgttgctt cggcaaacgc ctgggcgtca
     9961 agagaatata gtgtagacaa cagcagcata aagcgattga ccgctcgctt ttgctctgcg
    10021 tattcatccg aaagcagaag ttcacgcatt gcgcgaacct tgtctttaat cgttttgacc
    10081 ggcttcgctt cgacgatagc aggtgacgca acgcgaacct ctttcgtcac cggagcagca
    10141 ggctgtgatg cggcggaaaa tttcaacata cgccgtaaaa tgtcggatgc gctctcgccg
    10201 atatgcttag tgtggctggc aatatagctg tagagttcat catcaacttc aatcgttttc
    10261 atcttaatcc agtgcagtgt cttagctgaa tacaagtcgc caggagtata tgaataaatc
    10321 ccggcagcgg atagcgtcaa gcctggcttg cagaaaaact cggaataaac cttatttgtt
    10381 gcagccctgt ggcacaatgg tcaacataat accctaaccc gacaacgcga aaaaaagaac
    10441 tttgccatga aattgaatat ccgcgcgcaa actgcacaaa accagcacaa taattctccc
    10501 atcgttcttg tccacggtct gtttggcagc ctcgacaacc ttggcgtact ggctcgcgat
    10561 ctggtaaacg atcacaatat catccaggtt gatatgcgta accacggtct ttcaccgaga
    10621 gatccggtga tgaattaccc ggcgatggcg caggatcttg ttgataccct ggatgcactg
    10681 cagatcgaca aagcaacatt tatcggtcac tccatgggcg gtaaagcggt aatggcactt
    10741 actgcactgg cccccgatcg catagataaa ctggtggcga tcgatatcgc accggtcgac
    10801 tatcacgtac gccgtcatga tgagattttc gcggctatca acgcggtcag tgaatcggac
    10861 gcacaaactc gccagcaagc agcagcaata atgcgccagc atctaaatga agaaggggtg
    10921 attcagtttc tgttgaaatc ttttgttgac ggggagtggc gttttaacgt gccggtattg
    10981 tgggatcagt atccgcatat tgtgggttgg gagaaaatcc cggcatggga tcaccctacc
    11041 ctgtttatcc ctggtggtaa ttctccgtat gttagcgagc agtaccgtga tgatttactg
    11101 gcacaatttc cacaggcacg ggcgcatgtg attgcaggcg cgggtcactg ggtccatgct
    11161 gaaaaaccgg atgcggtatt acgcgctatc cgtcgctatc tcaatgatta acgcaactga
    11221 ttaaaaaccc agcgtccgcg tcgctttccg cgcgtgggcg ttggcgtgac tcatcgcgct
    11281 gatgtattat ggcgcgctat ccatccgggc agaggcctgg ctgattgttt cccccgaagt
    11341 caccaagatc atggccaaag aacaaacgga ccgtacgaca ttagatctgt tcgcgcacga
    11401 gcgtcgaccg ggacgaccga aaacaaatcc gctttcgcgc gatgaacagc tgcgtattaa
    11461 taaacgcaac caactaaaac gcgacaaagt acgtggcctt aagcgtgtcg aactgaagct
    11521 gaacgcggaa gctgtcgagg cgctgaacga gctggcggaa tcgcgcaata tgagccgtag
    11581 cgaactgatc gaagagatgt taatgcagca actggcggcg ctgcgtagtc agggcatcgt
    11641 ttaaatttcc actttcatgt agcacagtgt gcagtcctgc tcgtttgctg attccgcaag
    11701 actgcctgtt ctgctatgat tgcctttatc cgtgggcaat tttccacccc catttcaata
    11761 agtttcaaga ggttatttca ctcatggcta tcactggcat ctttttcggc agcgacaccg
    11821 gtaataccga aaatatcgca aaaatgattc aaaaacagct tggtaaagac gttgccgatg
    11881 tccatgacat tgcaaaaagc agcaaagaag atctggaagc ttatgacatt ctgctgctgg
    11941 gcatcccaac ctggtattac ggcgaagcgc agtgtgactg ggatgacttc ttcccgactc
    12001 tcgaagagat tgatttcaat ggcaaactgg ttgcgctgtt tggttgtggt gaccaggaag
    12061 attacgccga atatttctgc gacgcattgg gcaccatccg cgacatcatt gaaccgcgcg
    12121 gcgcaaccat cgttggtcac tggccaactg cgggctatca tttcgaagca tcaaaaggtc
    12181 tggctgatga cgaccacttt gtcggtctgg ctatcgacga agaccgtcag ccggaactga
    12241 ccgctgaacg tgtagaaaaa tgggttaaac agatttctga agagttgcat ctcgacgaaa
    12301 ttctcaatgc ctgatgtgat gcggcgtaga ctcatgtcta cgccgtatta atagataatg
    12361 ccaatcaaaa taattgctac aaatttgtaa cttttgctgt tgtacctgta caatgtcccg
    12421 gtgttcaagt ggccttgccg ttgtaaatgt aagctgtgcc acgtttttat taacaatatt
    12481 tgccagggac ttgtggtttt catttaggcg tggcaattct ataatgatac gcattatctc
    12541 aagagcaaat tctgtcactt cttctaatga agtgaaccgc ttagtaacag gacagattcc
    12601 gcatgactga taacaatacc gccctaaaga aagctggcct gaaagtaacg cttcctcgtt
    12661 taaaaatcct ggaagttctt caggagccgg acaaccatca cgtcagtgcg gaagatttat
    12721 acaaacgtct gatcgatatg ggtgaagaaa ttggtctggc tacggtatat cgcgtactga
    12781 accagtttga cgacgctggt atcgtcaccc gccacaattt tgaaggcggt aaatccgtat
    12841 ttgaactgac acagcaacat caccacgatc acctgatctg cctcgactgc ggcaaggtta
    12901 tcgaatttag tgatgattcc atcgaagcgc gtcagcgtga aattgccgca aaacatggca
    12961 ttcgcctgac taaccacagt ctctatcttt acggtcactg tgccgaaggc gattgccgcg
    13021 aagatgagca cgcgcacgaa ggcaaataag ccagtctgaa cgagaaaagc caacctgcgg
    13081 gttggctttt ttatgcaagg gaaataagaa aagcgagagt tacagctctc acttatttgt
    13141 tattactgcg aatttcctgc cagaccttat cacaatcggc tttcaccgcc tgatcatttc
    13201 cggtttgcgt tgcacgcaga cactgctgat aatccagtac gcggacactt tcctggtcgg
    13261 caaactgctg atgttgtttc tctttcttca gcacgtttaa cacgctctgg caggcttcaa
    13321 ttttttccgg cgaaccttgt gcggtgttga tacaggcgct gtatgcctct ttcagacggc
    13381 tgtcttcctg tggtgcggtt gattgcgcac aagctaccag ccccgatgcc atcatagcga
    13441 tgagaatcag ttttttcata gcaaattcct caaacgtgcg gcaggcacaa aacctgccgc
    13501 gtcgggcatc agaagatggt gaatggtgcg attaccataa attttacgtc acgctcatcc
    13561 tggaagatgt tgccgtaacc accgccccag cttgggatgt cggagtggtt gtcgtattcg
    13621 gtgaagtgca gtttgaacat cgtgcctttg gcgcgaccgt cctgaatggt gtagaccgca
    13681 tccaggctgt aggcagactc ttcaatagtc cggtttttgt cgtagtacgc atccggattg
    13741 ctctgccagg tcgcaggttt agcatcccat gcgtaaacgt aggaagcgcc gatggcgaag
    13801 cctggaagat tccagttttt caggtcatac atcgcaccga agaagaccgc tttttcgccg
    13861 ttggcgttga agtcagaacg gttatcccac cagatatcca ggcgaccgtt tgaggatgcg
    13921 taggtcggag tcatacgttg caggaagtat ccctgctgac cgtcagcttt aacccaggtg
    13981 ccttcgagac gcaaatccac tacgtcagcc gcccggtaac caaaggtcaa cgcttgcagc
    14041 caggcggtgc cgtcataaag atcgttgacg ctgcgatcgt caactttatc gcgggtaccg
    14101 tagaactggt agctggtggt taatgggcta ccggcgatat caaatttgta gctggctttg
    14161 gcaaaatatt gatcgatata ccctcctgcc tgaccaaatg ccgcttccag tacgaagtta
    14221 tttttgaagt cgtatttcgc cccaagggag tgcagataat caactttggt ggttttatcg
    14281 ttctgataaa actcatccat ttccagatgc cacggtgctt tgtattcgtt ggtccacatg
    14341 taggagaaac tcaacgcacc agcatcgccg taatcaaaat tcgctccggc ttccgccccc
    14401 tgataagtac ccggcataaa gctccagtga ggcgctaaca gcgtctgacc ggttggctga
    14461 atataacctg ccctcgccca aaccggaccg tatttaaatt tggccgcagc tttatacagg
    14521 cttataccgc ttttgtcgcc ggaccagtct tcgtcatagg ctttattact ttttgaaaac
    14581 gcgatttcgt tcgggtgaga gctgtcgccg ttttccgcca tttcaatcgc cgtaaacgca
    14641 gcaatatcaa ggccgaacat atcagcagca taaccggact gaaaatcgag gttggcattc
    14701 caggtggagt gagaaaggtt ggttttgtat ttgtcgccgt cggtaacatc tttacggtcg
    14761 cgttcacgct gccagtaata tataccgccg gttaaggttg aatcgtcgat gaatccttcg
    14821 gcgcgagcct ccggcattgc cataaagccc gacattgctg taacaccggc gatagccagc
    14881 gccagcgtac tacgtttgcc actaaacgta cgcatgggtt aatcctcttt gacgtataaa
    14941 ttgctgcacc aaaggtgaac agcgaaaaaa aataaaaatt aaaaaagtag cgttgagaaa
    15001 agtctcgctg actacaggaa
//
LOCUS       DAPGOF010000114        14931 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.115, whole genome shotgun sequence.
ACCESSION   DAPGOF010000114 DAPGOF010000000
VERSION     DAPGOF010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.115"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..156)
                     /locus_tag="RHV13_003167"
     CDS             complement(<1..156)
                     /locus_tag="RHV13_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242679.1"
                     /translation="MMKTITKQPILFTDVPVADLRNSMKQDLNQNLIERLWNIIRDFF
                     LDSDKQKA"
     gene            complement(387..1361)
                     /gene="yajO"
                     /locus_tag="RHV13_003168"
     CDS             complement(387..1361)
                     /gene="yajO"
                     /locus_tag="RHV13_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HDV1242680.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(1416..3278)
                     /gene="dxs"
                     /locus_tag="RHV13_003169"
     CDS             complement(1416..3278)
                     /gene="dxs"
                     /locus_tag="RHV13_003169"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDV1242681.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDETLILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            complement(3303..4202)
                     /gene="ispA"
                     /locus_tag="RHV13_003170"
     CDS             complement(3303..4202)
                     /gene="ispA"
                     /locus_tag="RHV13_003170"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HDV1242682.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            complement(4202..4444)
                     /gene="xseB"
                     /locus_tag="RHV13_003171"
     CDS             complement(4202..4444)
                     /gene="xseB"
                     /locus_tag="RHV13_003171"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDV1242683.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            4650..6098
                     /gene="thiI"
                     /locus_tag="RHV13_003172"
     CDS             4650..6098
                     /gene="thiI"
                     /locus_tag="RHV13_003172"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDV1242684.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDESQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNDFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            complement(6152..6742)
                     /gene="yajL"
                     /locus_tag="RHV13_003173"
     CDS             complement(6152..6742)
                     /gene="yajL"
                     /locus_tag="RHV13_003173"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HDV1242685.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(6705..7616)
                     /gene="panE"
                     /locus_tag="RHV13_003174"
     CDS             complement(6705..7616)
                     /gene="panE"
                     /locus_tag="RHV13_003174"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDV1242686.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            7784..8275
                     /gene="yajQ"
                     /locus_tag="RHV13_003175"
     CDS             7784..8275
                     /gene="yajQ"
                     /locus_tag="RHV13_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HDV1242687.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            complement(8403..9767)
                     /locus_tag="RHV13_003176"
     CDS             complement(8403..9767)
                     /locus_tag="RHV13_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1242688.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAVAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPANIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(9916..10806)
                     /gene="cyoE"
                     /locus_tag="RHV13_003177"
     CDS             complement(9916..10806)
                     /gene="cyoE"
                     /locus_tag="RHV13_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HDV1242689.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(10818..11147)
                     /locus_tag="RHV13_003178"
     CDS             complement(10818..11147)
                     /locus_tag="RHV13_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HDV1242690.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(11147..11761)
                     /locus_tag="RHV13_003179"
     CDS             complement(11147..11761)
                     /locus_tag="RHV13_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HDV1242691.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(11751..13742)
                     /gene="cyoB"
                     /locus_tag="RHV13_003180"
     CDS             complement(11751..13742)
                     /gene="cyoB"
                     /locus_tag="RHV13_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HDV1242692.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(13764..14711)
                     /gene="cyoA"
                     /locus_tag="RHV13_003181"
     CDS             complement(13764..14711)
                     /gene="cyoA"
                     /locus_tag="RHV13_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HDV1242693.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSQNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
BASE COUNT         3712 a         3933 c         3826 g         3460 t
ORIGIN      
        1 tgccttttgt ttatcactgt ccagaaaaaa atcacgaatt atgttccata gcctttcaat
       61 aagattttga tttaaatctt gcttcattga gtttcttaaa tctgcaaccg gtacatcagt
      121 aaacaggatc ggttgtttgg tgatggtttt catcatgatt gtaatcctct tacgttgact
      181 aaataagaca gttttagggg atattacctg gagaaactat caaaaatcgc tcaaagggat
      241 gaagacgatg aatagctgca aactcattca cgaagaattt ctcttgcaga ggtggattat
      301 ggttacagaa ggtgatggga cgtaccttgt ccccatcatc gcagacgatg gggaattcag
      361 cagataaaat gggtgaaaag cgaaatttac ttaaatccta cgaccggatg cggtttatac
      421 ggcgtttcca gttcggcaat ctgttctggc ttcaaagtga tatccaccgc gttcagcagc
      481 tcatcaagct gttcttcccg cgatgtaccg ataatcggtg cggcaatgcc cggtttactc
      541 aacaaccagg ccagcgcaac ttgtgctcgt gttgcaccga gttcttcact gacgcccgtt
      601 aaccgctccg caatttgcgc gtcattttca tcgctttctt tatagagatt tttccccacc
      661 tcatcagaca ccagtcgtgc ggtagtttct ccccacggac gagtcagtcg cccccgtgcc
      721 agcgggctcc atggaatcac cgctacgcct tcctgataac acagcggcag catttcgcgc
      781 tcttcttcac gataaatcag attgtagtga tcctgcatac tgacaaactg cgcccagccg
      841 tgctgttttt gtagttccag tgcctgagca aactgcgaag cgtgcattga tgacgcgccg
      901 atataacgcg ctttcccggc ttttaccacg tcgttgaggg cttccagcgt ctcttcgatc
      961 ggcgtgttgt aatcccagcg atgaatttgc aggatatcga cataatccat gccgagacgg
     1021 cgcaggctgt cgtcgataga gcgcaatatt tgcgcacggg ataatccttc cggcaaatca
     1081 ccaacgcgat ggaatacttt ggtggcaacg accacgtctt cacgacgggc gaaatcccgc
     1141 agtgcgcgac cgacgatctc ttcgctgctg ccatcggaat agctattggc ggtgtcaaag
     1201 aaatttatgc cgccttccag cgcgcgttta attatgggac ggctgctttc ttccggcagt
     1261 gtccatgcgt gattaccacg atcaggctcg ccaaaggtca tacagccgag gcaaagtcgg
     1321 gaaacgcgaa ggtcggtttt tcctaagggg ttgtattgca tgctgccact cctgccatga
     1381 ataatttctt aagcatagca ggagtggagt agggattatg ccagccaggc cttgattttg
     1441 gcttccatac cggcggcatc gaggccgagt tcggcgcgca tttcttcctg agttccttgc
     1501 ggaataaaga agtccggcag gccaatgttc agcacgggta ctggtttacg atgggccatc
     1561 agcacttcgt tcactccgct gcctgcaccg cccataatgg cgttttcttc tacggtaacc
     1621 agtgcttcat gactggcggc catttccaga attaatgttt catcaagcgg tttcacaaaa
     1681 cgcatatcga ccagcgtggc gttcagcgat tcggcgactt tcgccgcttc tggcatcagc
     1741 gtaccaaagt taaggatcgc cagtttctcg ccacgacgct tcacaatgcc tttgccaatt
     1801 ggtagttttt ccagcggcgt cagttccacg ccgaccgcgt tgccacgcgg gtagcgcacc
     1861 gctgacgggc catcgttata gtgatagccg gtatagagca tctggcgaca ttcgttttca
     1921 tcactcgggg tcataatgac catttccggt atgcagcgca ggtaagagag gtcaaaagcg
     1981 ccctgatggg tttgaccgtc agcaccaaca atgcccgcgc ggtcgatggc gaacaggacc
     2041 gggagttttt gaattgccac gtcatgcagc acctgatcat aggcgcgttg caggaaggtg
     2101 gagtagatcg cgacaatggg tttgtatcca ccaatggcca gtcccgcagc aaaggtcact
     2161 gcgtgttgct cggcaatcgc tacatcaaaa tagcgatccg ggaatttacg agaaaactcg
     2221 accataccgg aaccttcacg catcgccgga gtaatcgcca tcagcttgtt gtctttcgct
     2281 gccgtttcgc acaaccagtc gccaaagatt tttgaatagc tcggcaaacc gccgctactt
     2341 ttcggcaaac aaccgctgga gggatcaaat ttaggcacgg cgtggaaagt gattgggtct
     2401 ttttctgccg gttcataacc acgacctttt ttggtcatga tatgcaggaa ctgcgggcct
     2461 ttcaggtcgc gcatgttctt aagcgtggtg ataagcccca gcacatcgtg accgtcaacc
     2521 gggccgatgt agttaaagcc cagctcttca aacaacgtgc caggcactac catgccttta
     2581 atatgttctt cggtacgttt gagcagctct ttaatgggcg gaacgccaga gaaaactttt
     2641 ttcccgcctt cgcgcagtga agagtaaagc ttaccggaaa gcagctgtgc cagatggtta
     2701 ttgagcgcgc caacattttc ggaaatcgac atttcattgt cgttgagaac caccagcata
     2761 tcaggacgta tatcgcccgc gtgattcatc gcttcaaacg ccatgccagc ggtaatcgcg
     2821 ccgtcgccaa tgacacagac ggtacggcga tttttgcctt ctttctcggc agcaaccgca
     2881 ataccaattc cggcactgat ggaggttgat gaatgcccga cgcttaatac gtcatattcg
     2941 ctttcgccgc gccacgggaa cgggtgcaga ccgcctttct gacggatggt gccgattttg
     3001 tcgcggcgtc cggtcaaaat tttatgcgga taagcctgat gccccacatc ccaaatcaat
     3061 tggtcaaacg gggtgttata gacgtagtgc agcgccacgg tcagctcgac cgtgcccagc
     3121 ccggaggcga agtgcccgct ggaacggctc acgctgtcga gtaaatagcg gcgcagttcg
     3181 tcgcagagtt tcggtaaact ctctttcggc aacagtcgta actcctgggt ggagtcgacc
     3241 agtgccaggg tcgggtattt ggcaatatca aaactcatca ggggcctatt aatacttatt
     3301 gtttatttat tacgctggat gatgtagtcc gctagcgctt ccagtgccga ggtatcgagt
     3361 gattgttcag ccagttgttt cagcgactgg cgggcatcgt cgatcagatc ccgggctttc
     3421 ttccgggctt gctcaagacc cagaagtgca gggtaggtac ttttaccaag ttgctggtcg
     3481 gcaccctggc gttttcccaa cgttgcagta tctcccacca catccaggat gtcatcctga
     3541 acctggaagg caaggccgat gctctctgcg tacttgtcaa gtactggcag agcacgacgt
     3601 cctttatctc cggcgcttaa tgcaccaagg cgaacggcgg cgcgaatcaa tgcgccagtt
     3661 ttatgacgat gaatacgctc aagcgcgtcc agaggtacgt gtttgccttc cgcgtctaaa
     3721 tccaacgcct gaccaccgca cattccggca ataccgctgg cgctcgccag ttcagaaatc
     3781 atcgaaattc tgtcgcgatc cgacacttct ggcatatcgg catcgcttaa aatcgagaac
     3841 gccagcgttt gtaaagcgtc gccagcgaga atcgcgttcg cttcgccaaa cttcacatgg
     3901 caggtcggca aaccgcgacg cagatcgtca tcatccatcg ccggtaaatc atcatgaatt
     3961 aatgagtaag cgtggataca ctcaacggcg gcagcgggtg cgtccagcgt gtttgtgcta
     4021 acgccgaaca tatgaccggt ggcataaacc aggaaaggtc gcaggcgctt accacctaat
     4081 aatgcgccat actgcatggt ttcgaccacg ggagtgttct gaaagggcag tggggcgata
     4141 aaacggctca gcgcctggtt ggcctgctta acgcaggctt cgagttgctg cggaaagtcc
     4201 attactcatt gtccggtgta aaaggggtta gagaggcgtc ttcattgtca gacagcagaa
     4261 tttgtacgcg ctgttcggct tgttgtaatt tggcctgccc ctgacgtgcc agctgcacgc
     4321 cgcgttcgaa ctcgttcagc gcctcttcca gcggcaggtc gccactttcc agacgggtta
     4381 caatctgttc cagctcgctc agcgcctttt caaagctggc gggcgcctca tttttcttcg
     4441 gcataatgaa tgtctgactc tcaatatttt tcgccccgtc atggtaacgg actcagggca
     4501 aatagcaaat aacgcgcaat ggtaaggtga tgtgcgcagc aaagcgatgt tagtggtata
     4561 cttccgcgcc tggatgcagc cgcaggtgtg ggctgctgta tttttcccta tacaagtcgc
     4621 ttaaggcttg ccaacgaacc attgccgcca tgaagtttat cattaaattg ttcccggaaa
     4681 tcaccatcaa aagccaatct gtgcgcttgc gctttataaa aatccttacc gggaacattc
     4741 gtaacgtttt aaagcactat gatgagacgc tcgctgtcgt ccgccactgg gataacatcg
     4801 aagttcgcgc gaaagatgaa agccagcgtc tgactattcg cgacgccctg acccgtattc
     4861 cgggtatcca ccatattctc gaagtcgaag acgtgccgtt taccgacatg cacgatattt
     4921 tcgagaaagc gttggttcag tatcgcgatc agctggaagg caaaaccttc tgcgtacgcg
     4981 tgaagcgccg tggcaaacat gattttagct cgattgatgt ggaacgttat gtcggcggcg
     5041 gtttaaatca gcatattgaa tccgcgcgcg tgaagctgac caatccggat gtgactgtcc
     5101 atctggaagt ggaagacgat cgtctcctgc tgattaaagg ccgctacgaa ggtattggcg
     5161 gtttcccgat cggcacccag gaagatgtgc tgtcgctcat ttccggtggt ttcgactccg
     5221 gtgtttccag ttatatgttg atgcgtcgcg gctgccgcgt gcattactgc ttctttaacc
     5281 tcggcggcgc agcgcatgaa attggcgtgc gtcaggtggc acattatctg tggaaccgct
     5341 ttggcagctc ccaccgcgtg cgttttgtcg ctattaattt cgagccggtc gtcggggaaa
     5401 ttctcgagaa aatcgacgac ggtcagatgg gcgttatcct caaacgtatg atggtgcgtg
     5461 ccgcgtctaa agtggctgaa cgttacggcg tacaggcgct ggtcaccggc gaagcactgg
     5521 gccaggtgtc cagccagacc ctgaccaatc tgcgtttaat tgataatgtc tccgatacgt
     5581 tgatcctgcg tccgctgatc tcttacgaca aagagcacat catcaacctg gctcgccaga
     5641 ttggcaccga agactttgct cgcacgatgc cggaatattg cggtgttatc tccaaaagcc
     5701 cgacggtgaa agcggttaag tcgaagattg aagcggaaga agagaaattc gacttcagca
     5761 ttctcgataa agtggttgaa gaagcgaata acgttgatat ccgcgaaatc gcccagcaga
     5821 ccgagcagga agtggtggaa gtggaaaccg tcaatgactt cggcccgaat gacgtcattc
     5881 tcgatatccg ttctgtcgat gaacaggaag ataagccact gaaagtcgaa gggattgatg
     5941 tggtttctct gccgttctat aaactgagca ccaaatttgg cgatctcgac cagaacagaa
     6001 cctggctgct gtggtgtgag cgcggggtga tgagccgtct gcaggcgctc tatctgcgcg
     6061 agcagggctt taacaatgtg aaggtatatc gcccgtaatt tgtggttttt acgtcgcatc
     6121 tggtcagatg cgacgtttgc cgcatccgac actactcgta ataattataa atccctgccg
     6181 ccatcaccag ttgtgatgcc acttcatggg ctttttcacg cccaaccaac aggtcgataa
     6241 ttttcagacc aaagtcgata gctgtacccg gcccctggct ggtcagcaat tttacccgcg
     6301 catcccagac gacgcgcttg tcctgccatt gttcggcggg aattttgtct ttcagcgtcg
     6361 ggaagccggt catattgcca atcggaaaga tatcgtgcgg caccagcacg gtggctggcg
     6421 cggcgcaaat agccgcgacg atacgcccgg aacggtggaa ctgtttaacg gtttcaacca
     6481 gcagagtgct atcgcgaaaa cactccgtgc ctttaatgcc accaggcagc acgatcacgt
     6541 catattcgcc atcagccact tcgaccagcg gcgcatccgc cagcagcttc acgccgcgcg
     6601 agcaggtaat tgccaggttg ccatcgctgt cgacgctggc agtggtgact ttgataccgc
     6661 cgcgaaccaa gaggtcgatc gtggtgacgg cttcagtctc ttcactacca ggggcgaggc
     6721 aaaccagtgc cgatgcgctc atattcactc tcctttcttt ttaccatttc aaacaggcgg
     6781 gtgttttccg gtacggcaat cccatgcgcg cgggcgcggc gtaagagaaa accattgata
     6841 tagtcgattt cggtgtggcg cagcgcgcgg atatcctgca acatcgacga gatattttcc
     6901 gctgtggcat caatcacctg catcacgtaa tcacgcaaat cttccgctga agtatgatgc
     6961 ccttcgcgtt cgatcactgc cgcgacttct tcgcatattt gcataatttc ttgcggatga
     7021 tgacgtaatt caccgttcgg gcaattccag atggcagtca gtggattaat cacgcagttg
     7081 acggccagct tgcgccacag ctcggcgcga atattgttat gccaggcaac gtcaggcaac
     7141 acggtttgca aaatatccgc cagataactg taatcgccgt cctgttgccg tgccggacca
     7201 atatgcgtga taccgtttgc cacatgaata atgacattgc cgtcgcggcg ggcggcatgg
     7261 gtggtggtgc ccatcagtaa tggctgctga atgttttgca attcttcgat ggtgcccatg
     7321 ccgttgtgaa ttaacagtat tggcgtagtt acaggcagtg tggacgcgag gcttttgacg
     7381 gcatcggaaa cctgccatgc tttcagcgtc accaggagca gatcgctggt ggcaagaaaa
     7441 tcgggatcgt tggcggtcag cgattcgtta aatatcgaac catcagtctc aaccagattc
     7501 acactacaat aaggttgcgg tacgcgcagc cagccctgaa cttcgtgacc ctgtttacaa
     7561 agtgctgtaa gccataattg ccctaaggca ccgcatccca atacggtaat tttcattgtt
     7621 cctcctcacc cgcaaccact ccgggtgttc aataaggcta tcccttaatt gtgcatgctg
     7681 ttgcgactat gcacaattaa gggatacgtc ctggtgcagg actgttggtt atttaacttt
     7741 gcaggtatta tgcttcgcat caaaaatgaa gggagaggaa aagatgccat ctttcgatat
     7801 tgtctctgaa gttgatcttc aggaagcacg taacgcggtc gataacgcga gccgcgaagt
     7861 ggagtcccgt tttgacttcc gtaacgttga agcctcattt gagctgaacg acgccagcaa
     7921 aaccatcaaa gtgttgagcg agtccgactt ccaggtcaat cagttgctgg atattctgcg
     7981 tgccaagctg ctgaagcgcg gcattgaagg cagttcactg gatgtaccgg aaaatatcgt
     8041 tcatagcggt aaaacctggt ttgtggaagc gaaactgaaa cagggcattg agagcgccac
     8101 tcagaaaaaa atcgtcaaga tgatcaaaga cagcaaactg aaagtgcagg cgcaaattca
     8161 gggcgatgag atccgcgtga ctggcaaatc tcgtgatgat ttgcaggctg tcatggcgat
     8221 ggtgcgcggt ggcgatctgg gtcagccgtt ccagttcaaa aacttccgcg attaatcgcg
     8281 acaacctgcg ctttgttcat gtcagatgcg gcatgaacgc ttggttcggc ctacaaaggt
     8341 ttgtaaatca ggaacttagt aggcctgata agcgtagcgc agcaggcaat ttcgcctcgc
     8401 ttttatgcct ggcgaattgc ctgttcaacc tcaaagcgat tcgtcacttt gctgtcgatt
     8461 ttcacataag ctgaatgttc ttcttctgca atcaacactt ctttgatgcc ttcagtttct
     8521 agcaaacgca cttttaacgc ctcgtttgcg gcaatgttcg ccgggatttc aatgcgcaaa
     8581 ctgctgacat acggcggttc tttcatggta ctggcgactg ccagccacac tgcggccagc
     8641 attgcgccag cgagaaatac cccctgaccg tcaaacatgc cgtcaatcca gccgcccagc
     8701 gaaccgccaa tcgccacgcc aagaaactgg ctggtggagt aaacacccat cgccgtacct
     8761 ttgtaacctg ctggcgactc tttactgata agtgagggca ggagggcttc catcaaatta
     8821 aacgccacaa agaaaagctg cacgccgacc accagttgcc agaactgcgt ttgcgcgttc
     8881 cacaacacaa tttccgcaac cacgatcaac ccgacgcaga agacaaagac ttgcttcatt
     8941 ttgcgcttaa cttcagcgta gataatgaaa ggcacgaccg agccaaaggc gattagcatt
     9001 gtcgccagat agaccttcca gtgttcagcc gccgggaacc ccgcatcagc cagttgtccg
     9061 ggcagggcaa caaacgtcga catcagcaaa atatgcagac acataatgcc aaagttgagt
     9121 ttcagcagcc gcggttccgc cagcacttta ctgaaactgc ctttcaccat tccggactca
     9181 cgattaagta cgtgagtgct actgttgggc acaacccaaa tggtcaacgc aatgccggtc
     9241 gttgccagaa tagcgatcat ccagaacagc gcgtgcagcc caagtttgtg agtgatgatc
     9301 gggccaagca ccatcgcaac ggcgaaggta atgccaaagc tcacgccgat aaacgccatt
     9361 gctttggtgc ggttttgttc gcgcgtgaga tcggaaagca gcgccataac ggcagcggca
     9421 atcgcaccgg agccttgtag cgcccggccc agaataattc cccagatgga gtcagaaagc
     9481 gcagcgataa cgctaccggc ggcaaacact gccagcccac cgacaattaa tggtttgcga
     9541 ccaatgcggt cggaaagcag gccaaacgga atctgaaaaa cggcctgagt cagaccataa
     9601 ataccaatgg caataccgat taatgcttcg ctggcacctt gcagagccat gccgtacgtg
     9661 gtcagaaccg gcagaaccat gaacatgccc agcatgcgca acgagaatac ggtccctaaa
     9721 ccccaggtcg cgcgccgctc acctggcgtc attttataat cgttcattac cacctctgtt
     9781 ttaaattcac gactagtgta aagcggcaaa cgaatggggt aaacatgggg attttttagt
     9841 aaatatttcg agagggatat gtttctaatg ctaagaaaaa aggtgccgta gcaccttttt
     9901 aatagagagg ttttgttacc acacagcagc cagcagcgta tgcgagtccg gcaccataaa
     9961 atcaacggac atcatcaccg agagggcagt gatggcgatg atagagaagc cgaacagctt
    10021 gcgcgcccag attctgtcat cagcaacttt ataaccgcgc agagccatac ctaaccacca
    10081 gacgctaacc gccgcggcga ccaccagata tttataccca gcgtaaccgc caagagagag
    10141 catcagcgtg gcaacggcaa aggcgatgat atacagcgtg atgtgattct tcgccaccga
    10201 aatgcctttt accactggca ataccggaat gtttgccgcc tggtaatcct taaagcggaa
    10261 aatggcgatg gcataggagt gaggcatctg ccacaggctg aagatagcca gcaggatcgc
    10321 tgcgccgcta tcgaactcgc cggttactgc acagtagcca atcaccggcg gcgcagcgcc
    10381 ggagagcgaa ccaatcaatg tgccgtagac tgagtggcgt ttcatgtaca ggctataaac
    10441 gccgacataa accacaaagc ccatcacccc cagccagcag gccagcggat tcgcgccaaa
    10501 ccacagcagc ataaagccag caatacccag caaggtggcg tacaccagcg agacagcagg
    10561 agagatcagg cctttaacca gcacccgatt cttcgtcctt tccatctttc tgtcgatatc
    10621 cctgtcgatg tagttgttaa acacacaacc cgacgccaca accagtgaca ccccaaccag
    10681 cgtgtagata aacaggggat aatcaatgct gccctttgag gccagcagga atcccccaat
    10741 caccgagatc aggttgccaa agatgatgcc tggtttcgtt acttgcaggt attgcttaaa
    10801 catcataacc gccgctctta gtgcatcatc atgttgtagt tgaggttcca cataatccag
    10861 atggagccta caaccaggat agcgatgatt agcacggtga agacaaacgc cgtcatgttc
    10921 cagccttcat ctgatttggt attcatgtgc aggaagcaca ccagatgcac cagaacctgt
    10981 accactgcca ttgccaggat tgttcccaga attacggccg gagaggcagc ccctgtcatc
    11041 accatccaga acggaatcac cgtcagaatg atcgacagga taaagcctgt catgtaggtt
    11101 tttacgctgc catgggacgc gccgctgtga tcggtagaat gactcattac atcgccccca
    11161 tcagataaac aacagtgaac acacagatcc aaaccacatc caggaagtgc cagaacaggc
    11221 tcaggcacat gatgcgggta cggttagtgc tggtcaggcc gcgacgagcg atttgtacca
    11281 tcagcaccgc catccagata agaccggaag tgacgtgcag accgtgcgtg ccgaccagcg
    11341 caaagaacgc tgacaggaag ccgctgcgat ccggacccat gccgttaaca atcaggtgat
    11401 ggaattcgta gatttccatc ccgataaatc cggcgccaaa caaccaggtc aacgccagcc
    11461 aggagataac ctggcttttg ttgtttttgt acatggcgat agccgccatg ccgtaggtga
    11521 tggagctgaa caacagcaag aaagtttcaa ccagaacgaa cggcagttcg aaaatgtcct
    11581 tacctgtcgg gccgcctgcg gtgccgttca ccagaacggc ataggtagca aacaagatag
    11641 agaacagaat gcagtcgctc atcaggtaga tccaaaatcc gaagattttg gttccgcctg
    11701 catcgtggtg cccgtgttcg tgcgcgtggg cagtcgcgtg cgtcaaagta tcagttgcca
    11761 tttttcagcc ctgccttagt aatctcatcg aaatgctggt tttccagttt ttcgatttct
    11821 gccaccggca cgtagtaatc cacgtcctcg tcgaagcttt tcacgatcca ggtgatgatc
    11881 atgcctgcga agccaacaat cgccagccac cagatatgcc agatcatggc gaaaccgaag
    11941 atggtggaga aagccgcaat gacgatacct gcaccgctgt ttttcggcat atgaatttct
    12001 tcatagtggt caggcttttt atacgcttcg cctttctctt tcatttccca gaatgcatca
    12061 cgttcgtgaa cgtgcggcac aacggcaaag ttatagaacg gaggcgggga agaggttgcc
    12121 cactccagcg tacgaccacc ccacgggtcg ccagtcaggt cacggttctg gtcgcggtca
    12181 cgaatagaaa cgtacatctg aataacgagg cagagaatac ctagcgcaat cagtactgca
    12241 ccgctggctg caatcatcag catggtgtgg aactgcgggt caatctgctg gctcaaacga
    12301 cgggtcatgc ccatgaagcc cagcgcgtac agtggcataa aggcaacgaa gaagccgatg
    12361 atccagaacc agaacgcgcg tttaccccag gtttcgttca gtttgaaacc gaacgcttta
    12421 ggccaccagt aggtcatccc tgcgaagcag ccgaagacca cgccgccgat gataacgtta
    12481 tggaagtgcg caatcaggaa caggctgtta tgtagaacga agtccgcgcc cggtactgcc
    12541 agcagcacgc cagtcatccc gcccaccgag aaagtgacga taaaaccgat ggtccacagc
    12601 atcgcagaat ggaacacgat gcggccctga tacatggtga acagccagtt gaagatcttc
    12661 accccggtcg ggatggcgat aatcattgtg gtgataccaa agaaggcgtt tacgttcgcg
    12721 cccgcaccca tcgtaaagaa gtggtgcagc cagacgatga acgacagcac ggtgatacag
    12781 acggttgccc ataccagcga ggtataacca aacagacgtt tacgcgagaa ggttgccgca
    12841 atttcggaga acacaccgaa aacaggcagg atcaggatgt aaacttccgg gtggccccag
    12901 gcccaaatca ggttgatgta catcatcatg ttgccaccca tatcgttggt aaagaaatgg
    12961 gtgcccagat agcgatccag ggtcaacaac gcgacggtaa ccgtcagaat tgggaaggaa
    13021 gcaataatca gtacgttcgc gcacagtgat gcccaggtaa atactggcat cttgaacatg
    13081 gtcatgcccg gtgcgcgcat cttcagaatg gtaacgaaga agttgatacc ggtaagcgtc
    13141 gtacctatac cggatagctg gagactccat atccagtaat cgacaccgac tcccggactg
    13201 tactctattc ccgatagcgg tggataggcc agccagccgg tctgcgcaaa ttcgcccacg
    13261 ccgagagaaa cgttaaccag aatcacacca acaacggtaa accagaagct taagttgttg
    13321 aggaacggga acgcaacgtc acgcgcgcca atctgcagcg gaaccaccag gttcatcaga
    13381 ccgataacga aaggcatcgc cacgaagaag atcataatca cgccgtgcgc ggtaaagatc
    13441 tgatcgtagt ggtgaggtgg caggaagccc gcttcgcccg ccgaggcaag agcctgctgg
    13501 ctacgcatca taatggcgtc agcaaaacca cgcagcaaca tcacaatcgc cacgatgata
    13561 tacatgatac cgaggcgttt atggtcgacg gaggtcagcc actctttcca caggtaggtc
    13621 cacttaccga agtaagtgat caggccaacg agcgccagac ctcccaaaat aatgccagcg
    13681 atcgtaacca tgacgatagg ttcatggaac gggactgcat caagtgataa ttttccgaac
    13741 atctttattc ttcctcaacc cctttaatgg gcggattccg cgtggctcat gtccatgcct
    13801 tccatacctt cgtgtgcgct gtgctcacct tctggctggg tcatgtccat gctcttaccg
    13861 tgagccataa acttgttaat tacatcggca aacaagtctg gtttcacgtt ggagaaatat
    13921 tccacctggt tgtattcgct aggcgcggcc agtttttcga acgctgccat gtcagacatg
    13981 gtgttctgcg actgcttcgc ttttgcgacc cactggtcga attcggcgcg atccggtgtt
    14041 gcaatagctt tgaacttcat gcctgagaag cccgggccgc tgtagctggc ggagataccg
    14101 tcataagtgc cgggttcgtt ggcgatcaga tgcaggcgag tctgcatacc ggccatggca
    14161 taaatctggc tacccagacg cggaatgaag aaggagttca tcacggagtt ggaggtcact
    14221 ttgaagtaca ccggagtgtt cgccgggaaa gcgatttcat tcacggtagc aatgccctgt
    14281 tccgggtaga tgaagaacca tttccagtcc atggaaacca cttcgatggt aatgggcttc
    14341 tcgtcgtgtg ccagcggctt gctaggctca agagcgtgag tggttttcca ggtcagtacc
    14401 gcaaggaaga tgatgattaa gataggtacc gtccagacca cagcttccac tttattggag
    14461 tgtgaccagt tcgggctgta cttagcatct ttattgctcg cacggtactt ccaggcgaaa
    14521 ccaacagcca tcaagattgc gggaataacg acaatcaaca tcaggccaaa tgccgtcagt
    14581 atcagtgaac gttgctccag accaatctgt cctttgggat ctaacagcgc agaattacag
    14641 ccactgagca atacagtgcc tgcaaataat gacaaccatc ccaaactttt attgtatttc
    14701 ctgagtctca tttaacgacc tcaattccac gggatctggt ggcgtttaaa gtgtgtgggc
    14761 attttacggg aaggttacat tactgtaaac atgattaaat tcctgttact tggatttggc
    14821 tgtcaggtca catatgttgc aaaacatatc aggttgattt taagaagctg ttgcaaaagg
    14881 gcgttataac aaaaggggag tgcagaagga gtctgggcgg gcaattggta t
//
LOCUS       DAPGOF010000115        14876 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.116, whole genome shotgun sequence.
ACCESSION   DAPGOF010000115 DAPGOF010000000
VERSION     DAPGOF010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.116"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(200..2467)
                     /gene="adiA"
                     /locus_tag="RHV13_003182"
     CDS             complement(200..2467)
                     /gene="adiA"
                     /locus_tag="RHV13_003182"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HDV1242694.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKINESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGNHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(2666..3574)
                     /gene="melR"
                     /locus_tag="RHV13_003183"
     CDS             complement(2666..3574)
                     /gene="melR"
                     /locus_tag="RHV13_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HDV1242695.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3857..5212
                     /gene="melA"
                     /locus_tag="RHV13_003184"
     CDS             3857..5212
                     /gene="melA"
                     /locus_tag="RHV13_003184"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDV1242696.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            5315..6736
                     /gene="melB"
                     /locus_tag="RHV13_003185"
     CDS             5315..6736
                     /gene="melB"
                     /locus_tag="RHV13_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HDV1242697.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(6875..7504)
                     /locus_tag="RHV13_003186"
     CDS             complement(6875..7504)
                     /locus_tag="RHV13_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HDV1242698.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLKLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(7627..9273)
                     /gene="fumB"
                     /locus_tag="RHV13_003187"
     CDS             complement(7627..9273)
                     /gene="fumB"
                     /locus_tag="RHV13_003187"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HDV1242699.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSAADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(9351..10691)
                     /gene="dcuB"
                     /locus_tag="RHV13_003188"
     CDS             complement(9351..10691)
                     /gene="dcuB"
                     /locus_tag="RHV13_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HDV1242700.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(11262..11981)
                     /gene="dcuR"
                     /locus_tag="RHV13_003189"
     CDS             complement(11262..11981)
                     /gene="dcuR"
                     /locus_tag="RHV13_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HDV1242701.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(11978..13609)
                     /gene="dcuS"
                     /locus_tag="RHV13_003190"
     CDS             complement(11978..13609)
                     /gene="dcuS"
                     /locus_tag="RHV13_003190"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HDV1242702.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGLLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            13790..14020
                     /locus_tag="RHV13_003191"
     CDS             13790..14020
                     /locus_tag="RHV13_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HDV1242703.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGRLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            14032..14304
                     /locus_tag="RHV13_003192"
     CDS             14032..14304
                     /locus_tag="RHV13_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDV1242704.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            14531..14827
                     /gene="ghoS"
                     /locus_tag="RHV13_003193"
     CDS             14531..14827
                     /gene="ghoS"
                     /locus_tag="RHV13_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HDV1242705.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
BASE COUNT         3619 a         3526 c         3836 g         3895 t
ORIGIN      
        1 aaatttttat agtaatttct catgtacata tttttttaca gggtaaataa caggcaaaaa
       61 aaagcgcggg gtggggccgc gcaaaaagta tgacattttt gtttgcagca atttttatga
      121 aaacgttgcc atactcaaaa ggcctaaggc caggcggcgt aatgttattt aaacaattac
      181 gccttcagcg gaatagtggt tacgctttca cacacataac gtggtaaata ccgtcaataa
      241 tttcagtccc ttcagtttcg tgttcaaatc cagggaaatg atggtcccag gattgcagcg
      301 agcgtaaata acttacttgc ggactgtttt tatcgccgaa gttttcacca gacagcagca
      361 tcgggattcc tggcggatac gggataactg agtttgccgc gatgcgtcct ggtagatttt
      421 caatggatac cagttcgaca ttgttgtcga caatcgcgtt gtacgcttca cgcggggtga
      481 tttccgccac cggtaggccg gaataggctt cgttcaaccg tgcgccagga ttgttttctt
      541 tcagccaggc aaacatggtg tcacccagat cgtgaatccc catgttcgcg taagtgtcag
      601 gatattgttc aacaagttcc ggcatcacct gcgccagcgg tgtgttggcg tcatagtggc
      661 gtttgaagga gcaaagggtg ttaaccagag tcccccattt cccacgggtt acgcccatag
      721 agaacaggaa cataatttgg aagtcagtgg tgcgggtagg cacaatgccg tggcgaccaa
      781 gccaggcagt gaccagtgcc gccggaacac cggtttcttc cagttcacca tcttcaccca
      841 ttcccggagc aaggatgctg actttaatcg ggtcgagcat actccagtta tccggaatat
      901 ctttgaagcc gtgccagctt tcgcccggat gcattaccca gcagtcctga acggtggtca
      961 gcagtttggt tggtgcgtca gcaaagtcat aggttttgcc ggtttgtggg tcggtgacga
     1021 cttctttgtt ccacggtttg aagaaccagc taccgtcagc ggtgaactct ttatacagcc
     1081 gcgccatcgc ctgacggaaa tcaaccgctt cgtcgatcac ttcctgcgtc agtgacaggc
     1141 cgctgttgcc gtccatcatc gacaccgcca cgtcgttgga tgcgcagatg gcatacagcg
     1201 gggaggtggt ggcatgcatc atgtaggcct ggttgaagcg ggagaagtta atcgccccac
     1261 gaccttcacg tacatgaata taagaagcct gtgaaagcgc attcagcagt ttgtgggtgg
     1321 agtgggtggc gaaaacggta ggaccgttgt gattgccagg ttcgccgcgc atggcatagt
     1381 gatcggcata gatcgggttg aaacgtgcat agccgtacca ggcttcgtca aagtgcagac
     1441 gatcggaggt tttttccagc agatcctgcg cttctttagc gttataacac acgccgtcat
     1501 aggtgcagtt ggtcaccacg cagtaagacg gtttttgccc ggctttgtct ttagtcagcg
     1561 ggctttcatt gattttcttc tgcaaggttt caggttgcat ttcctgcgga tagattggcc
     1621 cgataatgcc gtagcggttg cggcttggca ccatgtagac tggtttcgcg cctgtcagca
     1681 tcaagccttg ttcgatggat ttatggcagt tacggtcaac gaccacgaca tcgttatcgg
     1741 tcatgcaagc ctgcatgatg gtgcggttag agccggaagt accgacgact accgaccagg
     1801 agcgatcggc accaaatacg cgtgcggcat atttttcgct ttcgccaaat gcgccagtat
     1861 ggtcaagcaa agaaccgagg gaagttcgtt cgatgcccat gtcggtgcgg aacagatttt
     1921 caccatagta gtcatggtag aaacgtccgg cgggtgtttt ggtaaaacca acgccgccct
     1981 ggtggcctgg cgctgcccag gaatattcat ggatgtcact atatttcatc agcgcgctga
     2041 acagtggcgg caacagctgc tggcggtagc gggtcatcgc ggcaacggcg cgtccggcga
     2101 taaagtcggc ggtatcttcc agaatccagg cgaattcatc gacaagctcc agcagatccc
     2161 ggtccattgc ggcgagggct ttttcccgat cgcccaacag gaagaccggc acgttttgtt
     2221 ggcgctcatg aagcttaccg atcaattgtc tgacgttttg atgttcgtcc ggatgttcca
     2281 tttgatagct gaacatcagg cagtcaatgg cttcgtttga agagagaatg gcaaaaccat
     2341 catcaaagga ggtggattta atcacggtaa cattttgctg gcttaaagca tctgccagac
     2401 gctcaacggc gttaccgacc caggtgtctt gatggagaaa ctcgctttca acaattaata
     2461 ctttcatcat tgcttacccg gttatgaagg aatcttcgtt gcgggcaagt atcttccggc
     2521 cttttcgggc tgtaaagcgc gtgaaaaaca aacattttta gaatatttat ttttataaac
     2581 aatatgttat gtgattattt taatttattt tcactgctta ttatcgacgg ctaaactatt
     2641 tttttggctg atactgatat cgtctttagc cgggaaacgt ctggcggcgc tgttggctaa
     2701 gtttgcggta ttgttgcggc gacatgccga catatttgcc gaacgtgctg taaaaacgac
     2761 tacttgaacg aaagcctgcc gtcagggcaa tatcgagaat acttttatcg gtatcgctca
     2821 gtaacgcgcg aacatggttg atgcgcatcg cggtaatgta ctgtttcatc gtcaattgca
     2881 tgacccgctg gaatatcccc attgcatagt tggcgttaag tttgacgtgc tcagccacat
     2941 cgttgatggt cagcgcctgg tcatagtttt cggcaataaa gcccagcatc tggctgacat
     3001 aaaattgcgc atggcgcgag acgctgtttt tgtgtgtgcg cgaggtttta ttgaccagaa
     3061 tcggttccca gccagagagg ctaaatcgct tgagcatcag gccaatttca tcaatggcga
     3121 gctggcgaat ttgctcattc ggactgttca attcctgctg ccagcggcgc acttcaaacg
     3181 ggctaagttg ctgtgtagcc agtgatttga tcaccatgcc gtgagtgacg tggttaatca
     3241 ggtccttatc cagcggccag gagagaaaca gatgcatcgg cagattaaaa atcgccatgc
     3301 tctgacaggt tccggtatct gttagttggt gcggtgtaca ggcccagaac agcgtgatat
     3361 gaccctgatt gatattcact ttttcattgt tgatcaggta ttccacatcg ccatcgaaag
     3421 gcacattcac ttcgacctga ccatgccagt ggctggtggg catgatatgc ggtgcgcgaa
     3481 actcaatctc catccgctgg tattccgaat aaagcgacag cgggctgcgg gtctgttttt
     3541 cgtcgctgct gcacataaac gtatctgtat tcatggatgg ctctctttcc tggaatatca
     3601 gaattatggc aggagtgagg gaggatgact gcgagtggga gcacggtttt caccctcttc
     3661 tcagagaggc gaggggagtg tccgagcatc atgaggccga aaactctgct tttcaggtaa
     3721 attattccca taaactcaga tttactgctg cttcacgcag gatctgagtt tatgggaatg
     3781 ctcaacctgg aagccggagg ttttctgcag attcgcctgc catgatgaag ttattcaagc
     3841 aagccaggag atctgcatga tgtctgcacc caaaattaca tttatcggcg ctggttcgac
     3901 gattttcgtt aaaaatattc ttggtgatgt gttccatcgc gaggcgctga aaacggcgca
     3961 tattgccctt atggacattg atcccacccg cctggaagag tcgcatattg tggtgcgtaa
     4021 gctgatggat tcagcagggg ccagcggcaa aatcacctgc cacacccaac agaaagaagc
     4081 cttacaggat gccgattttg tcgtggtggc atttcagatt ggcggttatg aaccttgcac
     4141 ggtgactgat ttcgaggtct gtaagcggca tggtctggaa caaaccattg ccgatacgtt
     4201 ggggccgggc ggtattatgc gcgcgctacg taccattccg catctgtggc aaatttgcga
     4261 ggacatgacg gaagtctgcc ccgatgccac catgctcaac tatgttaacc caatggcgat
     4321 gaatacctgg gcgatgtatg cccgctatcc gcatatcaaa caggtcgggc tgtgccattc
     4381 ggtgcaggga acggcggaag agctggcgcg tgacctcaat atcgacccgg ctacgctgcg
     4441 ttaccgttgc gcaggtatca accatatggc gttttacctg gagctggagc gtaaaaccgc
     4501 cgacggcagt tacgtgaatc tctacccgga actgctggcg gcttatgaag cagggcaggc
     4561 accgaagcca aatattcatg gtaatactcg ctgccagaat attgtgcgct acgaaatgtt
     4621 caaaaagctg ggctacttcg tcacggaatc gtcagaacat tttgccgaat atacgccgtg
     4681 gtttattaag ccaggtcgtg aggatttgat tgagcgttat aaagtaccgc tggatgagta
     4741 cccgaaacgc tgcgtcgagc agctggcgaa ctggcataaa gagctggagg agtataaaaa
     4801 cgcctcccgg attgatatta aaccgtcacg ggaatatgcc agcacaatca tgaacgctat
     4861 ctggactggc gagccgagtg tgatttacgg caacgtccgt aacgatggtt tgattgataa
     4921 cctgccacaa ggatgttgcg tggaagtagc ctgtctggtt gatgctaatg gcattcagcc
     4981 aaccaaagtc ggtacgctac cttcgcatct ggccgccctg atgcaaacca acatcaacgt
     5041 acagacgctg ctgaccgaag ctattcttac ggaaaatcgc gaccgtgttt accacgccgc
     5101 gatgatggac ccgcatactg ccgccgtgct gggcattgac gaaatatatg ctcttgttga
     5161 cgacctgatt gccgcccacg gcgactggct gccaggctgg ttgcaccgtt aaaacgcgac
     5221 taaacgctac tgcgccgggg gatttattcc ggcgcacacc tctgacgata ccaataacag
     5281 aaggcgggcg ttggtaacag cgacccgata ccctatgagc atttcaatga ctacaaaact
     5341 cagttatgga tttggagcgt tcgggaagga ttttgcgatc ggcattgtgt atatgtacct
     5401 catgtattac tacaccgatg tcgtcgggct gtctgtgggt ttggtcggta ctttgtttct
     5461 ggtggcgagg atctgggatg ctattaacga tccgattatg ggatggattg taaatgctac
     5521 gcgatcgcga tggggtaagt tcaaaccctg gatcctgatc ggtacgttgg caaactctgt
     5581 aatcttattt ctcctcttta gtgcgcatct gtttgaaggt actactcaga ttgtctttgt
     5641 ttgcgtgacc tacatcctct ggggcatgac ttacaccatt atggatattc ccttctggtc
     5701 gctggttcca accatcacgc tcgataaacg tgagcgcgaa caactggttc cttatccgcg
     5761 tttttttgcc agtctggcag gctttgttac ggcaggtgtg acgctaccat ttgttaatta
     5821 tgtcggcggt ggcgatcggg gatttggctt tcagatgttc acactggtac tgatcgcctt
     5881 ttttattgtt tcaaccatca tcactctgcg caatgtgcat gaagtctttt cgtcagacaa
     5941 tcaaccgtct gctgaaggaa gccatctgac acttaaagcc atcgttgcgc taatttataa
     6001 aaacgatcag ctttcatgcc tcttgggtat ggctcttgct tataatgtag ccagcaacat
     6061 tattaccgga tttgctatct attatttctc atatgttatc ggtgatgcgg atttgttccc
     6121 ctattatctg tcgtatgcgg gagctgctaa cctggtgacg ttagtattct tcccacgctt
     6181 agttaaatca ttatcccgac gcattttatg ggccggagca tctattcttc cggtgttaag
     6241 ctgtggtgtt ctcctgttaa tggcattaat gagctatcac aacgtcgtcc tcattgtgat
     6301 tgcgggtatt ttgctgaatg tgggaacggc gcttttctgg gtattacagg tcatcatggt
     6361 ggcagatacc gttgattacg gtgaatataa actgcacgta cgctgtgaaa gtatcgctta
     6421 ctccgtgcag actatggtgg tgaagggcgg ttcagccttt gcggcttttt tcattgcggt
     6481 tgtgttaggg atgattggct atgtaccgaa tgttgaacag tctacgcaag ccctattagg
     6541 tatgcagttt attatgattg ctctaccgac tctgtttttc atggtaacgc tgattctcta
     6601 cttccgtttc tatcgcctca atggcgacac gctgcgcagg atccagattc atctgctgga
     6661 taaatatcgc aaagtaccgc ccgagcctgt tcatgctgat attccggtcg gtgcagtgag
     6721 tgatgtgaaa gcctgacgtg gacaatagct aacgaaaatg gcctgatgcg atatgtttat
     6781 caggccacgt cttaatgtaa tgttttggat ttgcaaattt tgtagaccgg ataaggaatt
     6841 cacgccgcat ccggcatcaa caaagcgcac gttgttatcc ggttatcaag ccaaagcgcc
     6901 gtagctggcg gcaatgaaag cgagcgagga atatcaccgt cgttaatgcg ccaagcagcg
     6961 cgcagaacat atcggattgc gtgtcccact ggtcgccctg ggtgccgaga aaatcatccg
     7021 ctccctggcc cattgccagc gccgcccacc actcgattaa ttcatacatc gcgcttatcg
     7081 ccagcgccac gcaacagacc agaaacgcca ccattttacg tccgcgaacg tacatcccgc
     7141 gcacaaggat ctcacgtgcc accagtgcag gcaccagccc ctggaaaaaa tgccccagct
     7201 tgtcatacgg attacggctc aacttcagcc attcctgcac ctcaaaacca acggggactt
     7261 tcgcgtaggt gtattgtccg ccgaccatca ggatgatggc gtgaaagaaa atgagcgtat
     7321 agagcagcgg tgttaacgga taacgtttag cggtggcaag cagtagcggc acgacaataa
     7381 tcaccggtgt cacttccatc agccaggtga gtttgtcatg ggccgaaatg ccggtataaa
     7441 tcaggattaa cgttagcgcc agtgcgccgg tgttaagaat taacggcttg agtgtgcggg
     7501 tcatggtgag ttcgcaagtc agggaaaata ctgactattc accagcaagc gtgaaattac
     7561 aatcgcaaaa gaggtggccc agggggatca cctggcagca tgctgccagg cgctgggccg
     7621 aagaggttac ttagtgcagt tcgcgcactg tttgttgacg atttgctgga agaagtcgtt
     7681 acctttgtca tcgaccagga taaacgccgg gaaatcttct acttcgattt tccagatagc
     7741 ttccataccc agctccggat aagcgacgca ttccagatgc ttgatgctct gctgcgccag
     7801 taccgccgcc ggaccgccga tgctaccgag gtagaagccg ccgtgtttat gacacgcgtc
     7861 ggtaacctgc tgactgcggt tacctttcgc tagcatgatc atgctgccgc cgtgggattg
     7921 cagcagatcc acataggagt ccatacgacc tgcggtggtt gggccaagtg aacctgatgg
     7981 ataaccggca ggcgttttcg ccggacccgc gtagtagatc gggtgatctt tgatgtactg
     8041 cggaagttct ttaccggcgt caatcagctc tttcagcttg gcgtgtgcaa tatctcgacc
     8101 cacaataatg gtgccggtga gcgacaaacg ggtggatacc ggatattgcg aaagctgggc
     8161 gaggatctct ttcatcgggc ggttaaggtc gaccttcacc gcttcgcctt caccggcctg
     8221 gcgcagttct tgtggaatgt actgacctgg attgtgttcc agtttttcga tccagatacc
     8281 ttcgcggttg attttcgctt taatgttacg gtcagcggag caggagacgc ccatgcctac
     8341 cgggcaggat gcgccgtgac gtggcagacg gataacgcga atgtcgtgcg cgaagtattt
     8401 accgccaaac tgcgcgccaa gaccgagttt ctgggcctct tccagcagtt cctgttccag
     8461 ctggacatcg cggaacgcct gaccatgttc gttcccttcc gtcggcagtt catcgtaata
     8521 gtgagcgctt gctaacttga cggttttcag gttggtttcc gcagacgtac cgccaatcac
     8581 aaacgcgata tggtacggcg ggcaggctgc agtaccgagg gtacgcattt tctcgacgag
     8641 gaagtttttc agtttgccgg gagtcagcag ggctttggtt tcctggtaga gatacgtttt
     8701 gttggcagag ccgccgcctt tcgcaacgca aaggaatttg tactcatcgc catctaccgc
     8761 gtacaggtcg atttgtgcag gcaggttagt gccggtgttg acctctttgt acatgtctag
     8821 cgccgcgttt tgtgaatagc gcaggttatc ttcgatatag gtgttataga cgcctttcga
     8881 cagcgcttct tcatcaccgc cgccggtcca cacgcgctgg ccttttttac cgacgatgat
     8941 cgcggtgccg gtatcctggc aggtaggcag cacgcctttg gcggcgattt cggagtttct
     9001 taagaattgc agcgccacgt acttgtcgtt ttcgctggct tctggatcgt gaagaatagc
     9061 cgcaacctgt ttctggtgtg ccggacggag cataaaagaa gcgtcgtgaa aggcttgctg
     9121 cgccagcagg gtcagggctt ctggttccac tttcaggatg gtttcgccgt cgaagtcggc
     9181 agcgctaacg taatcggaag tgagtagata gtattcggta ttgtctttcc ccatcgggaa
     9241 aggtgcctgg tagataaagg gtttgtttga catagcttcc agcctgtaac tctgtatttg
     9301 ttattcgaaa atggcgtgcc gcgtagtcac gggcggcacg caaagtgcat ttataagaac
     9361 ccgtacatcg cggcgaagat ccagccgaag acgcacgata cgctcacacc aatcaacccc
     9421 ggcagaataa agctgtggtt gatgacgaag cgaccgatgt gggtggtgcc ggaacggtca
     9481 aactgaatcg ctgccagatc gctcggataa gtcggcagga tgtaataacc gtagcaagcc
     9541 ggtgctgaag ccacgatgta tgccggatca acgccgatcg ccagcgcgac cggaacaatt
     9601 gccgccagcg ccgcagcctg agagtttaca aacttggaaa ccagcagcag aacaatggca
     9661 taggcccacg gatactcttt caccatttca cccagtacgc cctgaatttc agacatatgc
     9721 gcaccgaaca tggtttctgc catccatgcg ataccgtaca ccgccacgat ggcgatcata
     9781 ccggaacgga agacttcgtt ttttgagata gacgcgggat tggttttggt caggataata
     9841 atcagcgccc cggtcagcag cataaacatc tgaataacca gtaccatcga cagcggtttg
     9901 ccgccgaagg atggacgcag gtccgaatca gcaccaagaa gtgcgactac agcgattgca
     9961 ccgaggaaaa tccacattgc cagccagttg cttttcggca gttttttatc gagcagcgtc
    10021 gcggtatcac cgtaaacata ctcacggttt tccggtacgg agatgaattt ctggaactct
    10081 tcgtctttat ccagatcttt accgcggaac cagctgaaga taccgatcgc caggataccg
    10141 attaacgtcg atggaatggt gattgccagc aggtcgagga actcaagatg gcgaccatca
    10201 aaggtgacat tacccagcat tgcaaccaga gacacgaccg caaccgacac cggactggcg
    10261 ataatcccca tctgtgcacc gatagaactt gccgccatcg gacgttccgg acggatgttg
    10321 ttcttaatgg cgacgtcgta gatgatcggc agaatggtgt aaaccacatg acccgtaccg
    10381 caaagaatgg tcagcgtaca ggtcacaaac ggcgcgacaa ttgagacata tttcgggttg
    10441 cggcgcagca gcttctcggc aatttgcagc atgacatcaa gaccgcccga agcttgcaag
    10501 gtcgccgatg ccgcaaccac cgcaatgata accagcatga catcaaccgg tggtttacct
    10561 ggctgaaggt ggaagacgaa gaccagaatg accagaccga taccgcctaa taaacccagc
    10621 gcgataccac cctttctggc accataaaac agacatatca gtattatgat aagttggata
    10681 gtaaataaca tgtgtgaacc ctcgcgataa tcctatttaa atttttgctg aatagatcac
    10741 agtcacgttc tgttttgtat gaactgtttt cagagcctga ctaaatatcc gtttggtcgt
    10801 taccggcttt agcaaatacc tcacagtgaa tattggctga ttaaagcagc ggcaaattta
    10861 tgcactgtga taagcggctt tttcagagag aaaaactctc tggcgatggg tttaaagttt
    10921 gtactgcgca gatgttaatg ttttgtttgc tgttatctta ttgatattta tcggtctgtt
    10981 ttttgttctc agggcagata tcattaggtt tatagattaa tctgatctac ccatttgtgg
    11041 gtaaaaatac acataatgcg ggtgacataa tagttaatta acttttgtta gcgttttgaa
    11101 attaaaaaca ccgttcacct gaagagatat taatttttag cgatgatgga gggataatta
    11161 tatttgatct ggcacaagtt ttactgatga aggatgtaac ttgtgccagg ggtattttgc
    11221 attacggtaa taattattac ttacagataa ccagcgatcc gttattggca atattgtttc
    11281 agtagtgagt agtgttctgc ctgaatacgg taacggtaaa ccggacgccc cgtgacgcca
    11341 taatggatac tggtgaacaa gatgtggcag ttgaccagcc agatgaggta tttacggcag
    11401 gaaacacgcg aaatgttaac ctcgttggct agctcgtcgg ttgaaaactc atagtcctga
    11461 tgcgcgtcaa tccactggca cagcgtgcgt aacgtctgcg gcgttaagcc ttttggcaag
    11521 cgacgaggat cctgctcgtt ggagctgctg ccgtggatta gctgatcaag ctcggcctgg
    11581 tcataatact gatgtttttc cagcgccatt ttcttttgcc gccagccggt gagcgcctct
    11641 tcaaagcggg aagcctggaa gggtttgatc aggtaatcca cgacaccgta atgcagcgaa
    11701 tctttaatgg ttgccgcatc ggctgcggag gagatgacaa tcacatcact tttgcaacgc
    11761 gcgttatgca ggacaggcag taaatcgagc ccgttctctt tttgcatata gatatcgagc
    11821 aatatcaggt cgataggcgt atcgctattg aagataatct ctttggcttt ctccagcgtc
    11881 gaggctgttc cacagcattg aaagcccggg atttgtgcta cgtatcggcg attcagctcc
    11941 gcgaccattg cgtcgtcatc gataattaat acattgatca tctgttcgac ctctccccgt
    12001 cccagggtat ctggacaaaa aattgtgtga aaatcccggg ttccgattcc acggcgatgc
    12061 tgccgccgag attttctacc tgttgtttga caagtgctaa accgacgcct cgctcgcttc
    12121 cttttgtcga gacacctttg tcaaaaatgt gatcgatttt gtcgggtgcg atccccggtc
    12181 catcatcgtt aacttcgcag tgcagccagc cgtgacggta gtgcaatgtt acgctaattt
    12241 cgccaccggg ttccggccct aatgcttcca gcgcgttttc tatcagattt cccaatgtgg
    12301 taatcagcgt cgcgacctgg tcctcactgc cgctgtctgg cagctggctt tcactgttta
    12361 aaatcagcgt atggcctaaa tcggtcgcgc ggttaatctt gctgattaat aaaccagcga
    12421 taaccggaga tttgatctta cccagcagag agccaatctc ttcctgatag ttattggctg
    12481 ttttgagaat gtaatcttcc aactgcttat aactcttcag atgcaataat ccgagaatca
    12541 catgcaattt attcataaat tcgtgggatc gttcacgaag tgcatcagca tagttgacca
    12601 gaccgtcgag tcgctgcatc agtttacgaa cttcagtttt gtccctgaag gttgaaatgg
    12661 caccgatgat aacgccatta ctgcgcaccg gaacggtgtt gatcagtaat agccggtctt
    12721 taatcgtaat ctcttcgtcg cggcgcggcg taccgtcgcg taacacttcc gagacatcta
    12781 ccacctgtga ccatgagtgg ctgagcgtcg acagtttctc atcgtcctgc gacttacggt
    12841 aattcagcaa ttcttgtgcg gcatcgttga tcagcgtgac ctcgccgcga tcgtccacgg
    12901 caacgacgcc ttctttgata gactgcaaca tggcctggcg ttgctcaaac agcgtggaga
    12961 tttcgtaggg ttccaggccg aaaaggattt ttttcagtac cttaaccaga atgcaggtgc
    13021 caatcagtcc gaccagcatg ccaaataata ccgaccagat aatgctccag cgactgtcat
    13081 tgatttgttg ggtcacgcgg cttaactcaa ggccgatcgc caccacgcca atttgtttat
    13141 gattttcatc gtagatgggg gtaaatacgc gtaaagcctg cgccagaaaa ccgcgattga
    13201 tagcgacatt ttcttcgcca ttcagcgctt taaggatgtc atcaccttta aatggctgac
    13261 caatacgctg ggcttcagga tgcgagtagc gaagactttg catatcggta acgacaataa
    13321 acagcagatc gttgcgtttg cgcacggctt ccgcgatggc ctggattcca ctctcctgcg
    13381 gttttttctg caagccctga cggatttccg gcgagtcggc gagagtacgc gccactgcca
    13441 gtgccttgtt ggccagcccg tctcgcgtca tatcactgat ttgcgagaag taaatcagat
    13501 gcaccaccaa tagcaccgag aacagtaccg cgctgaccat taagatcact gtggtactca
    13561 atttcatcgg acgtttgcgt aacatgcggt agggcaatga atgtctcatc agcttccttg
    13621 tgtgacaaat ttcttaagca ttatctctga tgaggcgggt aattcaaagg gagtaagaat
    13681 gattggctat ataggggaag agactctggc aacggaaact gccagtgctg tatgaagatt
    13741 acagggctat gcttatagcg ataatcatac tgatgagaga gggaaagtca tggatcaggc
    13801 gctactggac gagggttatc gctgttatac cggcgaaaag atcgatgtct atttcaacac
    13861 tgcgatatgt cagcattctg gcaattgcgt acgtggtaac ggcaggttat ttaacctcaa
    13921 acgtaagccg tggatcatgc cggatgaagt cgacgttgcc actgtggtta aagtgattga
    13981 tacgtgcccg agcggcgcgc tgaaataccg tcataaataa gcgagggtaa aatggaaata
    14041 cgcgaaggcc acaataaatt ttacattaat gatgaacagg gcaagcaaat cgctgaaatt
    14101 gtctttgtgc cgaccggaga gaatttagcg attatcgaac ataccgatgt cgatgaaagc
    14161 ctgaaagggc aagggattgg taaacagctg gttgcgaaag tcgtggaaaa aatgcgtcgg
    14221 gaaaaacgaa aaattatccc attatgtcca tttgcgaaac acgaatttga taaaacgcgg
    14281 gagtatgatg atattcgcag ttgatgggag agtacagatt cacgatattt ttcatgctct
    14341 ccacggtgtg atgcaggaga gcatctgaag ggtaggggga tgcacaaaga atgggcagag
    14401 agcgcgtttt tttgtcccaa gtcatcccct ttactgagca aaaaaaagaa tatctcctat
    14461 atgagaatca tcaatcgggg ttaataagtt ttgcgtcccc agagcattca atattgatag
    14521 gagtcatatt atggaaggta aaaacaagtt caatacttat gttgtttctt ttgattatcc
    14581 atcatcttat tcctcagtgt tcttaaggtt aagatcattg atgtatgata tgaatttctc
    14641 ctctatcgtg gctgatgaat atgggatacc acgacaattg aatgaaaact ccttcgcaat
    14701 aacgacatcg ttagccgcaa gtgaaatcga agatttaatc aggcttaaat gcttagactt
    14761 accggatatt gactttgacc tcaacattat gacagttgat gactatttcc gtcagtttta
    14821 caagtagcca ggaaggataa gaagaacata aaaaatggca ctattctcta aaatat
//
LOCUS       DAPGOF010000116        14766 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.117, whole genome shotgun sequence.
ACCESSION   DAPGOF010000116 DAPGOF010000000
VERSION     DAPGOF010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14766
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.117"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1022
                     /locus_tag="RHV13_003194"
     CDS             <1..1022
                     /locus_tag="RHV13_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HDV1242706.1"
                     /translation="QRDIIQGVKSFAKKNNFAITVFASHRNERHEILSVADYSLTEPE
                     DPQKRLQFIQETIQTYGIHHIHTGRNSQWFEEHRSAIESTGATLTTGATGVDWLTLAD
                     EKVTFAQFMEQNGLPVVPSWRVNTLAELKTHLAAPPFSDSPVCVKPVTGIYGMGFWRF
                     DDSASPMAVFNHPEHRLVSPQQYIAAASASESFKPLVLMPYLPGPEFSVDILADKGEI
                     LAAVGRRKEGAIQYLVNEGSAWELACDCARVMKADGLVNVQTRNDVNGNPVLLETNMR
                     PSGGVGYTLHSGVNLPGLFAAFKLGLMSEDAVIQSARNTFSPVAVRSITDVIVYPESL
                     SNHLN"
     gene            1032..2174
                     /locus_tag="RHV13_003195"
     CDS             1032..2174
                     /locus_tag="RHV13_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HDV1242707.1"
                     /translation="MSDTLEDMIYRRTLSCGTIQVTRDQGDVSLDDLFDIAERRNPKR
                     AFLFVSKVLGRHIPVPPSVMRQAYRQLASQFPATLTGPVLFIGMAETAVGLGAGVFDE
                     VRHQHRESVYLTSTRHPVDGTLLCEFKEEHSHATDHLIYLPDDEEKRRRVTNARTLVL
                     IDDEATTGNTFINLLSALRNTGKLQHIEQVIAVTLTDWSSNALSERTSLPVTSVSLVS
                     GQWGWTPLPDAPVPDMPKVNVTSRGEWDILGKQSWGRLGMLAPAADLGHEVSVRKGER
                     ILVLGTGEFVWEPFLLAERLEAAGAQALYGSTTRSPIAVGYAIESAISFTDNYGLGIP
                     NFVYNVAHQQFDRILVCTETPAESIDTQLLKALAEVAPVVEIVTYE"
     gene            2167..2940
                     /locus_tag="RHV13_003196"
     CDS             2167..2940
                     /locus_tag="RHV13_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242708.1"
                     /translation="MNKPVVLSDLDDTLFQTRRKMVDELALEPFRTGAVDRTLNPRSF
                     MTEEQSMLVDWLLEQAELIPVTARGTEEISRVRIPFHSWAITTHGAVILTPEGKPDEE
                     WKAHMLGQLAPYQEKLTSMQRLITEMMDAKGINAWARLNFEYGETAVYMVMKHRDSTR
                     LDELNAIADEIETVFPTEGFYIHRNSNNVAWLPTPVEKGLAVRWLLEKLRAERGVFPV
                     IGLGDSLSDHRFMKLCSWFGIPRQSQFADAISQRIFGEN"
     gene            2942..4021
                     /locus_tag="RHV13_003197"
     CDS             2942..4021
                     /locus_tag="RHV13_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HDV1242709.1"
                     /translation="MQPHDTFTGSYQPGDVEFLLKPVVIEMTPVEQKEELIQSGKKHY
                     SDMLSQEPAPTQWHLDLFHRALDRGAERLAKEVTQLAIALAKRFGDEPIVLASLVRAG
                     VPLGVMLHQALRDMGKTSWHYGISIIRDRGIDGAALDVIEERHGTSGIVFVDGWTGKG
                     AITGELVRALKDRPGYPEQPRLVVLADPCGCSWLAASDDDWLIPFGIMGAPVSGLISR
                     SVWSSEGLHGCMVCEHLSEFECSRMLVDTVAHFRKKLTPSSLATLSWNMESARVLWQT
                     SRDVIAFLADEFKVDSVNRIKPGIAEATRAVLRRVPDHVFVRSIDDPDVALLVGLARE
                     KGIVVTEMGGTLGQYRAVTIIKKVL"
     gene            4021..4977
                     /locus_tag="RHV13_003198"
     CDS             4021..4977
                     /locus_tag="RHV13_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HDV1242710.1"
                     /translation="MKNRLSPWNLGATLYMPATREDIADAVLHGKIPGLRSLVICLED
                     AVSEADIPIALKNLEHLLHELSNSMRSLGKNDWPLVFIRPRHAEMGRWLKAHYDLSAV
                     DGFVLPKFTLSSLAEWWDIMGGTHLCMMPTLETEDVFDVVQMRELATRLVEHPCHDRI
                     IALRIGGNDLMNVVSLRRPRDLTLYDSPMGYVIKMLVSVFGPRDFALTAPVCEHIDDH
                     AVMARELALDMAHGLVGKTAIHPGQIEVIQNALMVTQGEYSDALRILNSTQAVFKSQG
                     AMCEPATHRRWAAGILDRARFYGLQNEQSADGIRLLTVTQHH"
     gene            4988..6196
                     /locus_tag="RHV13_003199"
     CDS             4988..6196
                     /locus_tag="RHV13_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HDV1242711.1"
                     /translation="MGFRFRKSINIIPGVRLNLSNGAPSLSVGPRGASVSFGSRGTYA
                     NLGLPGTGLSYRTRLDRAARSGGGNRTATDPGLRQALEEEAADLMSAVTAIRNIHELT
                     PDPKTGISWAELEAVYLHNRTSPFQVPAPVRPEKPDYLALPEKPAESEGISFLGKWFE
                     SESAKAERHAENLRRWQQELIDVERENTLRQHRYQQQRTAWAEQYANWKFEAEEHEKR
                     LATAQADARQQFRTDAAFFESYLAGVLAETEWPRETLVAFEVKPELSAVLLDVDLAEI
                     EDFPDKIYGVNARGTELTEKAMTQKAVRENYARHVHGCLFRLVGIVLHTLPFDNVIVS
                     GFTQRVSKRTGYLEDEYILSCKCSRSQMSSVNFAGLEHIDPVEALGDHPVIRKMSSTF
                     IFQPIEPLTL"
     gene            6214..6681
                     /gene="terW"
                     /locus_tag="RHV13_003200"
     CDS             6214..6681
                     /gene="terW"
                     /locus_tag="RHV13_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance protein TerW"
                     /protein_id="HDV1242712.1"
                     /translation="MQLNTRQARIFKLANLLGTGKPVSAADIITSLECSEPTLTRALK
                     ELRESYSAEIKYSKAGHSYHLVNPGQLDKKTLRRMNEALAQNAELKTGESTGKVVLDK
                     DKKTAVSLSLRMRILRKIDRLAALSGSTRSEAVEKLALHSVDELIKEYSAKKS"
     gene            7144..7782
                     /locus_tag="RHV13_003201"
     CDS             7144..7782
                     /locus_tag="RHV13_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015731191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDV1242713.1"
                     /translation="MRRLPVYLLLDTSGSMHGEPIEAVKNGVQTLLTTLKQDPYALET
                     AYVSVITFDSSARQAVPLTDLLSFQMPALTASGTTSLGEALSLTASSIAKEVQKTTAD
                     TKGDWRPLVFLMTDGSPNDDWRKGLNDFKAARTGVVVACAAGHDADTSVLKEITEIVV
                     QLDTADSSTIKAFFKWVSASISVGSQKVESSKKEVIGLEDLPPPPPEVNVVL"
     gene            7805..8446
                     /locus_tag="RHV13_003202"
     CDS             7805..8446
                     /locus_tag="RHV13_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDV1242714.1"
                     /translation="MSVSLSKGQGVSLKKNEYDLSSVTIGLGWDINEEKKGFLGGIFG
                     KKEEEYDLDVIAFLCNSAGKVTDLGNVENGKPTLVNGDIIFFNSLRHKSGNIWLTGDN
                     RTGAGDGDDEQIIVRLNSLDAQYEKIVFIVQIYNGEKLQQHFGKVQNAFIRAVDARNI
                     EMARFDLSGGPAFASQRSMVFAELIREATGWKLRAIGEPSESDSFVSHLRNYM"
     gene            8446..9084
                     /locus_tag="RHV13_003203"
     CDS             8446..9084
                     /locus_tag="RHV13_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013087240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDV1242715.1"
                     /translation="MRRLPVYLVIDTSGSMRGESIHSVNVGIQAMLSALRQDPYALES
                     VHISIITYDNEAREFIPLTPLEDFQFSDIVVPSAGGTFTGAALECLMQCVERDVRRSD
                     GDTKGDWRPLVFLMTDGTPSDALAYGEAVKAIRGRGFGSIIACAVGPKAGHEHLKQLT
                     DKVVSLETLDSTAFAGFFKWVSASVSSGSTSAGINNGTDTLPPPPPEIQLVL"
     gene            9170..10210
                     /locus_tag="RHV13_003204"
     CDS             9170..10210
                     /locus_tag="RHV13_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDV1242716.1"
                     /translation="MRRLPVFFVLDCSESMIGENLKKMTDGLQMIIGDLRKDPHALET
                     AWVSVIAFAGVARTIVPLHEIASFYPPRLPVGGGTSLGAALRELTVQIDTQVRKTTHE
                     AKGDWKPVVYLLTDGRPTDDTTAEVKRWKDHYASKVNLIAVGLGPSADLNILRQLTEN
                     VMLFTESQEGDFTRFIKWITASVTAHSRSVGDDKQPELSQTEYIVRLAKDEPVKAYDE
                     NCVTLTGRCSKTRRPYLMKYERPPARVSGLDFSLNLNSFNIAGCYPIDEDYFAWSDAT
                     ATGLQVNTSELHGVPGCPHCGNASAFALCSCGKLLCIDGPDDVICPWCETGLSFSNDG
                     GNTNFDVNRGRG"
     gene            10210..11847
                     /locus_tag="RHV13_003205"
     CDS             10210..11847
                     /locus_tag="RHV13_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HDV1242717.1"
                     /translation="MSQNALLEEKIIRLVLSELGHPVEGERLLLLSQDPSLTEEIMRL
                     KERIESLAQGLSARVSEGDNDAEIAASSASSLSDQQQTVVSVADNPGIDATDTSALQP
                     LPENEPHPPFWKLMPYYEFDKPAQREDENLSSLVLKPGQIPTGQQRAGLAVPPAVPPR
                     AKIVIPNARAGERFSSPVAIVLDEGQQATVRDVVFPRNIGLTFDKEHKLLTGTPTESG
                     DIELSVLWSCASHDECETKQLFIVNPDPRSLWKVVEPPADASYPKSHLDAAGLVRGDI
                     RIAAASRRGRSHEHAGSFRDDDFYINHCQETGWSVMLVADGAGSAVNSREGSRIAVQT
                     AGDYLFNQLSGLKGVHLKQHITAWEGSDQQATINAMLHHFKQAATLAVNSIQNEAICA
                     EQPVKSYSTTLLATVALRTDNELFAAAFWLGDGAIGAYSPSGKVRILGNPDSGEYAGQ
                     TRFLDQSIIADPSFSGRISVGKWNDVSHLILMTDGVSDPLFETDNGLRSDEKWTRLLD
                     ELIPVLTDASIAPERLGDWLNFFSTGNHDDRTIAVLW"
     gene            11873..13372
                     /locus_tag="RHV13_003206"
     CDS             11873..13372
                     /locus_tag="RHV13_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012303502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HDV1242718.1"
                     /translation="MANIVTCKTKDGETVQYVDEVIGSGSMKDVYFSPDKSYVVAFYH
                     KPQNEQARDRIDMITGRYRQNIFGQSGGEYWKDLFCWPTHVVEHGDKIGIVVPTYKSY
                     FFFKYGSKNDDFLGIKGREKEGKWFASASNQNKFLDPRERGNTLTYLKVCLLLTRAVR
                     RMHAAGLCHSDLSYKNVLIDPEMGHACIIDVDGLVVPGKYPPDVVGTPDFIAPEVVKT
                     SHLSKEDPNRVLPSISTDRHALSVLIYMYLFFRHPLRGGKIHDMSDEVRDETLSMGEK
                     ALFIEHPTDKSNAVKVSQLSSFSLPWADPEKIPYTIMGPYLTPLFERAFIDGLHDANK
                     RPTADEWESALVKTVDLIQPCQNKACEQKWYVFSGKTKPVCPYCGTPYKGKLPVLNLY
                     SSRKEGSYRPDDHRLMVWSGQSIYAWHVNRLIAPNERTTDAQRKRVGYFVFHNDQWWL
                     VNEGINGLMSLPDKRQIAIGEKIELTNNAQFVLSKEEGGRLVVVQLVEN"
     gene            complement(13539..14621)
                     /locus_tag="RHV13_003207"
     CDS             complement(13539..14621)
                     /locus_tag="RHV13_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242719.1"
                     /translation="MRKSYTFGIPFGLQRESGLFLDITEVSRGIDCNCICPACKTDLL
                     AKQGEVKLWHFSHSTAVAGDCDGLMEAIRGKIIEVINEHQVLGFPNLLAGDDGGPVSL
                     NEVSGSGSMFGGTADLFVKVNDLCVAVFLDRDRSISEKLTFDHLHPSERVAALRIDLP
                     DIEYEISQVQLGRRKTTYSECIESLIIDETSSREWLYHPLMHELESEPLQEYRGREPS
                     EEGPLLQRLADCKMKLPDILPGSHNALVRLQQDTIVCLCRLTVRAYDIAQTDEFPLFL
                     RYFRLHCLENSEHVSYERLICWQEMISKTQEGQSLTQNELEYLHEIVRIAFYNHRLNN
                     VSISDVKNHDTGKKSGRVESQNYSLL"
BASE COUNT         3637 a         3751 c         3819 g         3559 t
ORIGIN      
        1 cccagcgaga tattattcag ggggtaaaat catttgcaaa aaaaaataat tttgccatta
       61 ccgtttttgc ctcccaccgt aacgaaagac atgaaattct ttccgttgcc gattattctt
      121 tgactgaacc tgaagatcct caaaaacgtc ttcagtttat tcaggaaacc attcagacct
      181 acggcatcca ccatattcat actggccgta acagccagtg gtttgaagaa caccgttcag
      241 ccattgaatc gaccggagcc acccttacta ccggtgcaac aggcgtcgac tggttaactc
      301 tggctgacga aaaagttact tttgctcagt ttatggagca aaacgggctc ccggtcgtac
      361 cgtcctggcg ggtgaatacg ctggcagaat taaagacgca cctcgcggcc ccgccgttct
      421 cggacagccc ggtatgcgtg aagccggtga cgggtatcta tggcatggga ttctggcgct
      481 ttgatgacag tgcttcgccc atggccgtct ttaatcatcc cgaacatcgt ctggtcagtc
      541 cgcaacagta tattgcagca gcatcggctt ctgagtcgtt taaacccctt gttttgatgc
      601 cgtacctgcc aggcccggaa ttttccgtcg atatcctcgc ggataagggc gaaatactcg
      661 cagccgtggg acgccgtaag gaaggggcta tccagtatct ggtaaacgaa ggaagcgcct
      721 gggaactggc ttgtgactgc gcccgggtta tgaaggccga cgggctggtg aatgttcaga
      781 cgcgaaacga tgtgaatggc aacccggtgc tgcttgaaac caacatgcgt ccgtcaggcg
      841 gggtgggtta tacccttcac agcggggtga accttcccgg gttgtttgct gcctttaagc
      901 tcggtctgat gtctgaagat gcggtgatcc agagcgccag aaataccttt tctccggttg
      961 cagtgagatc catcacagat gtcatcgttt acccggaatc actctctaac catctgaatt
     1021 aaggcgtgtt tatgtctgat accctcgaag atatgattta tcgccgtacc ctctcatgcg
     1081 gtacgataca ggtaacccgc gaccagggtg atgtttcgct cgatgacctg tttgatatcg
     1141 ctgaaagacg taacccgaaa cgcgcctttc tgtttgtcag caaagtactc ggcaggcaca
     1201 ttcccgtgcc accctcagtc atgcggcagg cctacagaca gcttgccagc cagttccccg
     1261 caacgctgac agggcccgta ctttttatcg gtatggctga aaccgccgtt gggctagggg
     1321 ccggtgtgtt tgatgaagtg cgccaccagc atcgtgaatc tgtctatctg acctctaccc
     1381 gtcacccggt tgatggcacg ttactttgcg agttcaagga agaacacagc cacgccaccg
     1441 atcatctgat ctatctgcca gatgatgaag agaaaagacg tcgtgttacc aacgcacgaa
     1501 cgctggtttt gattgatgac gaggcaacca ccggtaatac ctttattaac ctgctttctg
     1561 ccctgcgtaa taccggcaag cttcaacata ttgaacaggt tatcgccgtt acgcttaccg
     1621 actggagtag caacgccctg tcggagcgca catccttgcc cgtcacttcg gtttcgcttg
     1681 taagcggtca gtggggatgg accccattac ctgatgcacc tgtcccggac atgccgaaag
     1741 tcaacgtaac ttcacgcgga gaatgggaca tcctgggaaa acagtcctgg ggtcgactgg
     1801 ggatgctcgc acctgcggcc gatctcggcc atgaggtctc cgtccgcaag ggggaacgta
     1861 ttctggttct cgggaccggg gaattcgtct gggagccgtt cctgcttgct gaacggctcg
     1921 aagctgccgg agcacaggca ctttatggtt cgaccacccg ctcccctatc gccgttggtt
     1981 atgccattga gtccgccatt tcctttacgg ataactacgg gctgggcatc cccaattttg
     2041 tctataacgt cgcccaccag cagtttgacc gtattcttgt gtgtactgag acacccgcag
     2101 aaagtattga cacgcagctt cttaaagccc tggctgaggt tgcgcccgtc gtggagattg
     2161 ttacctatga ataaacccgt tgttctgagc gaccttgacg acacgctctt tcagacccgt
     2221 cgtaaaatgg tggacgagct ggccctggag ccattccgta ccggtgccgt tgatcgcacc
     2281 ctgaatccac gaagctttat gacggaagaa cagtccatgt tggtggactg gctcctggag
     2341 caggctgaac tcatacctgt taccgcccgg ggaactgaag aaatcagccg cgtccggatc
     2401 cctttccact cctgggcaat caccacgcac ggggccgtca ttctcacgcc tgaaggcaaa
     2461 cccgacgagg agtggaaggc ccatatgctt ggccagctgg ctccctacca ggaaaagctg
     2521 acatcgatgc agcgtctgat cactgaaatg atggacgcaa aagggatcaa tgcctgggca
     2581 aggctgaact tcgaatacgg tgaaacggcg gtttatatgg tgatgaagca ccgcgacagc
     2641 acccgccttg acgagctcaa tgccattgca gatgagatag aaacggtgtt tccgaccgag
     2701 ggcttctaca tccaccgcaa cagtaataac gtggcctggc ttcccacccc ggttgagaaa
     2761 gggctggccg tcagatggct gcttgaaaaa cttcgggctg aacgcggagt cttccccgta
     2821 attggcctgg gcgacagcct gagcgatcat cgttttatga aactgtgcag ctggtttggc
     2881 atcccgcgtc agagtcagtt tgcagatgcc atttcacagc gaatttttgg agaaaattaa
     2941 aatgcagcca catgacacat ttaccggctc ataccagccc ggtgacgtgg aatttctgct
     3001 aaagccggtt gtcattgaga tgacgccggt tgagcaaaaa gaagagctga ttcagtcagg
     3061 gaagaaacat tattcggaca tgctcagtca ggagccagcg ccaacacaat ggcatcttga
     3121 tttgtttcac cgggcgctgg atcggggcgc agagagactt gcaaaagaag tcacacagct
     3181 cgctattgct ctggccaaac gctttggtga tgagcccatt gtactggcca gtcttgtcag
     3241 ggctggcgtg ccgctcggcg ttatgctgca ccaggccctg cgtgacatgg ggaaaacctc
     3301 atggcattac ggtatcagca ttatccggga tcgtggaatt gacggtgcag ccctcgacgt
     3361 cattgaagag cgccatggta ccagcggtat tgtctttgtt gacggatgga caggtaaagg
     3421 cgcaattacc ggagagcttg tacgcgcact gaaagatcgc ccgggctatc ctgagcagcc
     3481 gcgactggtt gtcctggccg atccctgtgg ctgctcctgg cttgcagcca gtgatgatga
     3541 ctggctcatt ccttttggca tcatgggtgc gccggtatca ggcctgatct cccggtcagt
     3601 atggtcctct gaaggattac atgggtgcat ggtttgcgag catctcagtg aattcgaatg
     3661 cagccggatg cttgtcgata ccgtcgccca tttccgtaag aagttaaccc cgtcatccct
     3721 cgctaccctg agctggaata tggagtcagc ccgggtctta tggcagacaa gtcgcgacgt
     3781 tatcgcgttc ctggccgatg aatttaaagt ggacagcgtc aatcgtatta aacccggtat
     3841 tgctgaagca acccgggctg tattacgtcg ggtaccggac catgtatttg tgcgttctat
     3901 tgacgacccg gacgttgcct tgcttgtagg gcttgctcgt gaaaagggaa tagttgttac
     3961 agaaatgggg ggaaccctcg gccagtatcg ggctgtaacc attatcaaga aggtactctg
     4021 atgaaaaatc gtctttctcc ctggaatctg ggagccacgc tatacatgcc tgcaacacgg
     4081 gaagatattg ctgatgccgt cctgcacggg aaaatcccgg gtttgcgctc tctggtgatt
     4141 tgcctggagg atgctgtcag tgaagcagac atccccatcg ccctcaaaaa tctggagcac
     4201 ctgctgcacg agctcagtaa cagcatgcgt agcctgggta aaaatgactg gcctctggtg
     4261 ttcattcgcc ctcggcatgc cgaaatgggc agatggttaa aagcgcatta tgatctttcc
     4321 gctgtcgatg ggtttgtgtt gccgaaattt accctcagtt cgttagctga atggtgggat
     4381 atcatgggcg ggactcacct gtgcatgatg ccaacgctgg aaacagaaga cgtctttgac
     4441 gtggttcaga tgcgcgagct ggccactcgc ctggtggaac atccctgcca cgaccgcatt
     4501 attgcgcttc gaatcggcgg caacgatctt atgaacgttg tgtcgcttcg ccgcccccgg
     4561 gacctgacgc tgtatgacag cccgatgggc tacgtcatca aaatgctcgt ttcagtcttc
     4621 ggcccgcgtg attttgccct gaccgcgccc gtatgtgagc atattgacga tcatgccgtt
     4681 atggccagag agctggctct tgatatggca catgggcttg ttgggaaaac ggccattcac
     4741 ccgggtcaga tagaggtcat tcaaaacgcg ctgatggtca ctcagggtga gtattctgac
     4801 gctctgcgga tcctgaactc tacccaggcc gtgtttaagt cgcagggagc aatgtgtgaa
     4861 cccgccacac atcgccgctg ggcggctggc attctggaca gagctcgttt ttatgggtta
     4921 cagaacgagc aaagcgctga tggaatcaga ttacttaccg tgacccagca tcattaagga
     4981 aatcataatg gggttcaggt tcagaaagtc gatcaacatt attcctggcg ttcgcctcaa
     5041 cctgagtaac ggtgcaccga gcctgagtgt cgggccgaga ggtgcttccg tttcttttgg
     5101 tagccggggg acctatgcca atctgggctt gcccggtacc gggctgagtt accgtacccg
     5161 gcttgatcgg gccgcgcgtt ccggaggtgg gaaccggacg gctaccgacc cggggctcag
     5221 acaggcgctt gaggaggaag ccgctgatct catgtcagcg gtgaccgcaa tccgtaatat
     5281 tcacgagctg acgccggatc ctaaaacagg catcagctgg gcagagctgg aagcagtata
     5341 cctgcataac agaacgtcgc cttttcaggt tccggcaccg gtgcgtccag aaaagccaga
     5401 ctatcttgca ttgccggaaa agcctgccga gagcgaaggc attagttttc tgggtaaatg
     5461 gtttgaatcg gaatcagcta aagctgagcg ccacgccgaa aatcttcgcc ggtggcagca
     5521 ggagctgatt gatgtggagc gtgagaatac ccttcgacag caccggtacc agcaacaacg
     5581 gacggcctgg gccgaacagt atgcaaactg gaagtttgag gctgaagaac atgaaaaacg
     5641 gctcgccacg gctcaggcag atgcccggca gcagttccgg acagacgccg cgtttttcga
     5701 atcatacctg gcgggtgtgc tggcagaaac tgaatggccg cgtgaaacgc ttgttgcatt
     5761 tgaagtaaag ccggagctat cagcagtcct gctggatgtt gatttagctg agattgaaga
     5821 tttccctgat aagatttacg gcgttaatgc ccggggcacg gagctgacgg aaaaagccat
     5881 gacgcaaaaa gccgtacgcg aaaactatgc ccgccacgtc catggctgct tgttccgcct
     5941 ggtcggtatc gttttacata cgctaccgtt cgacaacgtg attgtgtcag gctttacgca
     6001 acgggtcagt aagcggaccg gctatctgga ggatgagtat atcctgtcct gcaaatgcag
     6061 tcgcagccag atgtcgtcag taaattttgc aggcttagaa cacattgatc cggttgaagc
     6121 gttaggcgat cacccggtta ttcgaaagat gagcagtacc ttcatttttc agcctattga
     6181 accactaacc ctttaagcag gctaaatctt acgatgcaat taaataccag acaggcccgg
     6241 atttttaagc ttgccaactt actgggcacc ggcaagcccg tttccgctgc cgacattata
     6301 accagtctgg aatgctcaga gccgacatta acccgggcac tcaaagagct acgcgagtca
     6361 tattctgcgg agatcaaata cagcaaggca ggtcattcct accatctcgt taatccgggc
     6421 cagctggata aaaagaccct tcgccggatg aatgaagcgc tcgcacagaa tgctgaactg
     6481 aaaaccggtg agtccacagg gaaggtcgta ctcgataagg ataaaaaaac agccgtgtca
     6541 ttatcgctac gcatgcggat tttaagaaaa attgaccgtc tggctgcgct gagtggctcg
     6601 acccgaagtg aagcggtaga gaagctggct ctgcactccg ttgatgaact gataaaagaa
     6661 tacagcgcta aaaagtcctg acgctaaatc tcagaccggg gtgaaaaccc cggttttgtc
     6721 atatttcacc tctgtatctg cgtgacggag ttttgatcta ttctcctccc gcaatgtcag
     6781 cagcgccttt ctgcctacaa aacctggtgt cttgacatcc tcttgtgcta cacatcaatc
     6841 tttgggcgat ttacactcca ttcatgcaga tctgtgatgt atgatgattt tcatacataa
     6901 gaaagtttat ttttgccgga gtttttttca gaaatttata ttccagtacg gtctgaattt
     6961 ttttctctga taagaggcca ttcacatgtc ggtaattcaa tggtggttaa cttatttctt
     7021 taatgccgct tctgagtatt taatctccac ccgacagatg tcgtgccgtt ttatcaaatc
     7081 tacaaactct ggcctcttcc tgaaactact ctcctcttcc gatctcttaa gaaggaatta
     7141 catttgagac gcttacccgt atatctgcta ctcgacacgt ccggctctat gcacggagag
     7201 cctatcgaag cggttaagaa tggcgttcag acgctgctta ccacgctgaa acaggatccg
     7261 tatgcactcg aaacggctta cgtgtcagtg atcacctttg attcttcagc ccgacaggca
     7321 gtccccctga cagacctcct gagtttccag atgccagcac taacagccag cggcaccacc
     7381 tctcttggcg aagcgctttc cctcacggcc agctccattg ccaaagaagt acagaaaacg
     7441 acggctgaca ctaaaggtga ctggcgtccc cttgtattcc tgatgacgga tggaagtccg
     7501 aatgatgact ggcgtaaagg cctgaatgac tttaaagcgg ccagaaccgg cgttgttgtg
     7561 gcatgtgcag ccgggcatga tgccgatacc agcgtcctca aagaaatcac tgaaatcgtg
     7621 gttcagctcg atacagctga cagttcgacg attaaagctt tctttaaatg ggtcagtgcg
     7681 agcatttcgg taggcagtca gaaagtggag tccagcaaaa aagaagtgat cggtcttgaa
     7741 gacctgccac cgccgccgcc agaagtaaac gtggtcttat aattttatta attaagggga
     7801 tgtcatgtct gtcagtctga gcaaaggcca gggcgtaagc ctgaaaaaaa atgaatacga
     7861 tctctcgtcc gttactatcg gcctgggttg ggatattaat gaggaaaaga aaggtttcct
     7921 cggcgggatc tttggtaaaa aagaagaaga atacgacctt gatgtgatcg ctttcctgtg
     7981 taattcagcc ggaaaggtga ccgatctcgg caatgtggaa aatggtaaac caacgcttgt
     8041 gaatggcgat atcatctttt tcaacagcct tcgccataag tcaggaaata tctggctgac
     8101 gggcgataac cgaaccggag ccggtgatgg tgacgatgag caaattattg tgcgcctgaa
     8161 ttcccttgac gctcagtacg agaaaattgt gttcatcgtt cagatctaca atggtgaaaa
     8221 gctccagcag cactttggta aagttcagaa tgccttcatc cgggcagtag atgcccgtaa
     8281 tattgaaatg gcacgattcg atctttctgg cggacccgcc ttcgccagcc agcgctccat
     8341 ggtttttgcc gagctgatac gcgaggctac aggctggaaa ctcagggcaa ttggtgagcc
     8401 ttcagaatca gattcgtttg tctcgcacct gaggaattac atgtgatgcg ccgcctgcct
     8461 gtttaccttg ttatcgacac atccggctcg atgcgcggtg agtccatcca ttccgttaac
     8521 gttggaattc aggcgatgct tagcgctctt cgccaggatc cctacgccct cgaaagcgtt
     8581 catatctcca ttatcaccta tgacaatgag gcgcgtgagt tcattcctct cacgccgctg
     8641 gaagacttcc agttttctga catcgttgtg ccaagcgcag gcgggacgtt caccggtgct
     8701 gcccttgaat gtctgatgca gtgtgttgaa cgggatgtac gtcgctcaga tggtgataca
     8761 aaaggagact ggcgtcctct ggtattcctg atgactgatg gaaccccttc tgatgccctg
     8821 gcgtacggtg aagcggtaaa agcgattcgc ggccggggat tcggatccat cattgcctgc
     8881 gccgttggtc ctaaagcagg ccatgagcac ttaaaacagc tcaccgataa ggttgtgtct
     8941 ctggagacgc tagattcaac cgcgtttgca ggtttcttta aatgggtatc ggccagcgtg
     9001 tcttccggca gcacaagcgc ggggatcaat aacggaactg atacccttcc ccctcctcca
     9061 ccagaaatcc agctggtgct ctgacagaca gaaaggtgct gctttccggg cagcaccttt
     9121 ctgtacagca acgttttctg ctggccgtac cagccgtacg aggaaaatta tgagacgcct
     9181 tcccgtgttt tttgtcctgg actgttcaga gtccatgatt ggtgaaaacc tgaaaaaaat
     9241 gactgatggt ctgcaaatga tcatcggaga tttaagaaag gatccacacg cacttgaaac
     9301 agcctgggtt tcggtaatcg catttgccgg tgtagcccgt acgattgtac ctcttcacga
     9361 aattgcctcg ttctaccctc cccgccttcc cgttggcggc ggtacgagcc ttggggccgc
     9421 attgcgtgag ctgaccgtac aaattgatac ccaggtcaga aaaaccactc atgaggctaa
     9481 aggcgactgg aaacccgtcg tgtatctcct taccgacgga cgtccgactg acgacacaac
     9541 cgcagaagtg aagcgctgga aggatcacta cgcgagtaaa gtgaatctca ttgccgttgg
     9601 cctggggccg tcagcggacc tgaatatcct gcggcaactg acagagaatg tcatgctgtt
     9661 caccgaatct caggaagggg actttacccg cttcatcaaa tggatcacgg cctcggttac
     9721 ggcgcacagc cgcagcgtcg gggacgacaa acaacctgag ctcagccaga ctgaatacat
     9781 agtccggctg gccaaggacg agccagtaaa agcgtacgac gaaaactgcg ttacgcttac
     9841 cggccgttgc agcaagaccc gtcgtccgta cctgatgaag tatgaacggc caccagcaag
     9901 ggtttccggg ctcgatttca gtctgaacct gaacagcttt aatattgccg gatgctatcc
     9961 catcgatgag gactattttg cgtggtcaga tgccaccgct accggtttgc aggtaaatac
    10021 cagcgaactg catggcgtac cgggttgccc ccactgcggt aatgccagtg cgtttgccct
    10081 gtgctcatgc ggaaagttgc tgtgtattga cggcccggat gacgtgatct gcccgtggtg
    10141 tgaaacaggc ctgtcattca gcaatgatgg cggaaacact aacttcgacg taaacagagg
    10201 gagaggttga tgtcgcaaaa tgctttactt gaagagaaga ttatccgctt agttctgtct
    10261 gagctgggcc accctgttga aggtgagcgg cttttacttc tatcgcagga cccatccctt
    10321 accgaagaaa ttatgcggct taaagagcgg attgagagcc ttgcacaggg tttatctgcg
    10381 cgggttagcg aaggtgataa cgacgcagag attgccgcct cgtcagcatc cagtctttcc
    10441 gatcagcaac aaacggtcgt aagtgttgct gataacccgg gcattgacgc taccgataca
    10501 tcagcactgc aacccctgcc agaaaatgag cctcatcctc cgttctggaa gctgatgcct
    10561 tattacgaat ttgataaacc agcacagagg gaagacgaaa acctgtcttc cctggtatta
    10621 aaacccgggc agataccgac aggccagcag cgggccggat tagctgtgcc acctgccgtt
    10681 cccccgagag ctaaaatagt tattcccaac gcccgcgcag gcgaacgttt ctcttcgccg
    10741 gttgctatag ttctggatga gggccagcag gctactgtca gggacgttgt ctttcccagg
    10801 aacattggcc tgacctttga caaagagcat aagttgctga caggtacacc caccgaaagc
    10861 ggcgatatag agctttcagt actctggtca tgcgcttcgc acgacgaatg cgaaacaaaa
    10921 cagcttttta tagtcaatcc cgatccgaga agcctgtgga aggtggtaga gccaccagca
    10981 gacgcttcct accctaagtc ccatctggac gctgctggcc tggtcagggg tgacatacgt
    11041 attgctgccg ccagtcgccg gggacgatct catgagcatg ccgggagctt cagagatgac
    11101 gacttctata tcaaccactg ccaggaaaca ggatggtctg tcatgctggt cgcagacggg
    11161 gccggtagtg cggtaaattc ccgggaaggc tcccggattg cagtccaaac ggctggcgat
    11221 taccttttca atcagctcag tggtttgaaa ggcgtccatc taaagcagca catcacggcg
    11281 tgggaaggga gcgatcagca ggcaaccata aacgccatgc ttcatcattt taaacaggca
    11341 gctacgctcg cggtcaacag tatccagaac gaagccattt gtgcagaaca acctgtgaaa
    11401 tcctattcaa caaccctact ggcaactgta gcgctacgaa ctgataacga gctgtttgcg
    11461 gctgcattct ggcttggtga tggtgcgata ggtgcataca gcccttccgg taaagtcagg
    11521 atactgggaa atcccgatag cggagaatac gcaggacaaa cccgctttct tgaccagagc
    11581 attatcgcag acccctcgtt tagcggccgc atcagcgtgg gtaaatggaa tgatgtatct
    11641 cacttgatcc tcatgacaga tggcgtatca gaccctctgt ttgagacaga taacgggctt
    11701 cgcagtgacg aaaaatggac ccgtctcctt gatgagctca tccctgtcct tacagacgcc
    11761 agtattgcgc ctgagaggtt aggcgactgg cttaactttt tctccacagg gaaccacgat
    11821 gaccgcacta ttgcggtgtt gtggtaaggg atacgcttct tcggtgatca atatggcaaa
    11881 tattgttacg tgcaaaacaa aggacggcga aacagtccag tatgttgacg aggtaattgg
    11941 ttcgggctcg atgaaggatg tttacttctc gcctgataaa tcatacgtcg tcgcttttta
    12001 tcataaaccg cagaacgagc aggcccggga tcggattgat atgatcaccg gacgctacag
    12061 gcaaaacatt tttggccagt ccggaggtga atactggaag gacctgtttt gctggccgac
    12121 ccacgttgtt gagcatgggg ataaaatcgg catcgtggtt ccaacctaca aaagctactt
    12181 tttctttaaa tacggctcta aaaacgatga ttttcttggc ataaaaggcc gtgaaaagga
    12241 aggcaaatgg tttgccagcg ccagcaacca gaataaattt ctcgacccac gtgaacgagg
    12301 caatacgctt acctatctca aggtctgtct gctgctgaca agagccgtca gaaggatgca
    12361 tgcggcaggt ctctgtcaca gcgatctaag ctataaaaac gtgcttatcg atccagaaat
    12421 gggacatgcc tgcatcattg acgtagacgg cctggttgtc cctggaaagt atcctcccga
    12481 cgtcgtgggc accccggatt ttatcgctcc ggaagtggtg aaaaccagcc atctttccaa
    12541 agaggatccg aaccgcgtat tgccaagcat ttctactgac cgtcatgcgc tgtcggtgct
    12601 tatctacatg tatctgttct tccgtcatcc gctacgtggc ggaaaaatac atgacatgtc
    12661 ggatgaagta cgtgatgaga ccttatctat gggtgagaag gcactcttca ttgaacatcc
    12721 gacagacaaa agcaatgcag tcaaagtcag tcagctatcc tccttttcac tgccctgggc
    12781 tgacccggag aaaattcctt acaccatcat gggcccttat ttgacgcctt tgtttgaacg
    12841 cgcctttatt gatggcttac acgatgccaa taaacgcccg accgccgatg agtgggaaag
    12901 tgccctggtt aaaacagtcg atctgataca gccctgccag aacaaggcgt gtgaacagaa
    12961 atggtacgtt ttctcaggta agacaaagcc ggtttgtccc tactgcggta cgccatacaa
    13021 gggtaaatta ccggttctaa atttatattc atcccgaaag gaaggcagtt atcgtcctga
    13081 cgaccaccgg ttgatggtgt ggagcgggca gtcaatctat gcgtggcatg tgaatcgcct
    13141 cattgcgcca aatgagcgca caaccgatgc acaaaggaaa cgagttgggt attttgtttt
    13201 ccataacgat cagtggtggt tagtaaatga aggcataaat gggcttatgt cattaccgga
    13261 taaacgacag attgccattg gggaaaaaat tgaactgacg aataacgctc agtttgttct
    13321 gtcaaaggag gaaggcggca ggctggtcgt cgttcagtta gttgaaaact aacggacgcc
    13381 cattacgact aaaacgttct ggcagcatac tgaacctgtt taatgaatca ctatattaag
    13441 cagtgtcaaa tttaagctgc cagaaaaaac tgactgtctg tgccggatat gcggtacacc
    13501 gcctgcggca agggtttaat gcgcaaagcc caagttattt ataaaagtga atagttttgt
    13561 gactctaccc tgccgctctt ctttccagtg tcatggtttt taacatctga aatactgaca
    13621 ttgttaagcc ggtgattgta aaaggcgatg cggacaatct catgaagata ttcaagttcg
    13681 ttttgtgtca aggactggcc ttcctgggtt tttgaaatca tttcctgcca gcagatcagt
    13741 ctttcgtaac tgacatgttc gctgttttcc aggcagtgca acctgaaata gcgaaggaac
    13801 aaaggaaatt catcggtctg agcgatgtca taagcccgaa ccgttaaccg acacagacat
    13861 acaatcgtat cctgctgtaa cctgactagc gcgttatgac tgcccggaag gatatcaggc
    13921 aatttcatct tacagtcagc aaggcgctga agaagaggcc cttcctctga aggctcccgt
    13981 ccccgatact cctgaagagg ttcagattca agttcatgca ttaatggatg atatagccat
    14041 tcccgggaac tggtctcatc aatgatcagg ctttcaatgc actcactgta tgttgtttta
    14101 cgtcgcccga gctgcacctg actgatttca tattcaatat caggaagatc gatacgcaat
    14161 gcggcaactc tttcggaagg atgaagatgg tcaaaggtta acttctctga aatgctgcga
    14221 tccctgtcca gaaagacggc cacacataaa tcattaacct ttacgaacag atcagctgtg
    14281 cctccgaaca tactgccgct tccactaacc tcattcagtg aaaccggccc accatcgtcg
    14341 ccagcgagaa gatttgggaa accaagaacc tggtgctcgt tgatgacttc aataatcttt
    14401 ccccggatgg cttccatcag accatcacag tcaccggcta cagctgtact gtgtgagaaa
    14461 tgccaaagct tgacctcccc ctgctttgct aacagatcag ttttgcaagc ggggcagatg
    14521 cagttacagt caataccccg gctgacctct gtaatatcta aaaagagtcc tgattctcgc
    14581 tggagtccaa atggaatacc aaatgtataa ctcttacgca tggctgcacc ccgtttttga
    14641 taatgattca aactatatcc gaaataacgg aggaggattt ctgtaaaacg tattaaaaga
    14701 aatgattttt ccgtcttcca gattaatgtt tgacaaaaat caaatgagag ccttacaaat
    14761 attgag
//
LOCUS       DAPGOF010000117        14694 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.118, whole genome shotgun sequence.
ACCESSION   DAPGOF010000117 DAPGOF010000000
VERSION     DAPGOF010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14694
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.118"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(49..1503)
                     /gene="xylB"
                     /locus_tag="RHV13_003208"
     CDS             complement(49..1503)
                     /gene="xylB"
                     /locus_tag="RHV13_003208"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HDV1242720.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMAAVPVIAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLSNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(1575..2897)
                     /gene="xylA"
                     /locus_tag="RHV13_003209"
     CDS             complement(1575..2897)
                     /gene="xylA"
                     /locus_tag="RHV13_003209"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HDV1242721.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            3263..4255
                     /gene="xylF"
                     /locus_tag="RHV13_003210"
     CDS             3263..4255
                     /gene="xylF"
                     /locus_tag="RHV13_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV1242722.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            4333..5874
                     /gene="xylG"
                     /locus_tag="RHV13_003211"
     CDS             4333..5874
                     /gene="xylG"
                     /locus_tag="RHV13_003211"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HDV1242723.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTDGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            5852..7033
                     /gene="xylH"
                     /locus_tag="RHV13_003212"
     CDS             5852..7033
                     /gene="xylH"
                     /locus_tag="RHV13_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HDV1242724.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKALNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRRRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            7111..8289
                     /gene="xylR"
                     /locus_tag="RHV13_003213"
     CDS             7111..8289
                     /gene="xylR"
                     /locus_tag="RHV13_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XylR"
                     /protein_id="HDV1242725.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVLL
                     "
     gene            complement(8397..9221)
                     /locus_tag="RHV13_003214"
     CDS             complement(8397..9221)
                     /locus_tag="RHV13_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HDV1242726.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            9541..11571
                     /gene="malS"
                     /locus_tag="RHV13_003215"
     CDS             9541..11571
                     /gene="malS"
                     /locus_tag="RHV13_003215"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HDV1242727.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQIDTRSGTPT
                     LMISIQNAAEPVANQVRVCPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDVPAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGSEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDELKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            11749..13002
                     /gene="avtA"
                     /locus_tag="RHV13_003216"
     CDS             11749..13002
                     /gene="avtA"
                     /locus_tag="RHV13_003216"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HDV1242728.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLHEKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(13153..13626)
                     /locus_tag="RHV13_003217"
     CDS             complement(13153..13626)
                     /locus_tag="RHV13_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDV1242729.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQSRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRATGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(13728..13868)
                     /locus_tag="RHV13_003218"
                     /pseudo
     CDS             complement(13728..13868)
                     /locus_tag="RHV13_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
     gene            complement(13870..14685)
                     /locus_tag="RHV13_003219"
     CDS             complement(13870..14685)
                     /locus_tag="RHV13_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV1242730.1"
                     /translation="MLELSMTLPIKVQNGGLFISRGVGRHPARRLESWEIIFVEKGRL
                     TIQEEERIFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDNDPRWLTHLS
                     VPQQTKVVDPQALVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADGAG
                     TTLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFRKHTGLTPAAWKRRYSKEHINS"
BASE COUNT         3730 a         3759 c         3607 g         3598 t
ORIGIN      
        1 atatatagat gtgaattatc ccccacccgg tcaggcaggg gataacgttt acgccattaa
       61 tggcagaagt tgctgataga ggcgacggaa tgtttcacgt cgtggctgat aagcggcata
      121 acgctgcgca tctggtagat gcgactgttc taacggtagt tgcggcaaca actcaatgag
      181 cgatttctct ggattcgccg caatctgcgc cagccttgct gcgcccagtg ctggccccac
      241 atcccccccc gtacggtaat cgagctgctg accgctgata tccgccagca tctgacgcca
      301 gtactcacta cgcgccccgc caccaatcaa cgtaacactt tgcggtttaa tgccgcaggc
      361 atgcacgaca tccatgccat ctgccagcgc ataacccacg ccttccagca ctgctcgcgc
      421 cagttcattg gggccatgtt gatgagtcaa accaaagaaa acccccttcg cctggggatt
      481 attgtgtggc gtacgctcgc cggaaagata aggcagaaac caaactggct cggcactttc
      541 atcagcctgt tgagctgcag cgattaaagc tgggacattg ctcaggccgg ttaatttcgc
      601 ggcccaatcc agacatgacg ctgcactcag cattacagac attaaatgcc aacgctgcgg
      661 tagcgcatgg caaaagctgt gtacggcgct ttctggcttg cttaaaaacc cttcgctgac
      721 agcaaaatag acccccgacg tccccagcga taacattgcc tgattagcat caaccattcc
      781 cacaccaacc gcaccagctg cattgtcgcc accgcctgcg ataactggca ccgccgccat
      841 accccacgct ttcgcaactt caggtaacaa agcaccagta atttcgctgc cttcgtataa
      901 tgcgggcatc tggtcacgag ataagtcgca agcctgcagc atgacgtcac tccagtcacg
      961 ctttgcgaca tccagccaca tggtgccagc tgcgtcagac atatcgctgg caaactcccc
     1021 cgtcatacgc agacgcaagt aatctttcgg taataatact ttgtcgattt gacggaatat
     1081 ctccggctca tgccgctgaa cccatagcaa tttaggcgca gtaaatccgg gcatcatcag
     1141 gttgccggta atcactcgtg attgcggaac tctcgcttcc agcaaagtgc actcttgcgc
     1201 acagcgcccg tcgttccaca aaatggcagg gcgcaatacc cgttgttgag catccagtaa
     1261 ggttgctcca tgcatctggc cggcaatacc caatgcttta acgtcctgca gagaatgctg
     1321 atcgcccaga gctttcattg cgcgatcagt tgcctgccac cactgttccg ggtcttgttc
     1381 cgaccagagt ggatgcgggc gcgaaacggt cagcttttcc gtttgcgaag caaccacctc
     1441 accctgctcg ttgagcaaaa taacttttac gcccgaggtg ccaagatcta tcccgatata
     1501 catatcgatc gttccttaaa aaaatgcccg gtatcgctac cgataaccgg gccaacggac
     1561 tgcacagtta gccgttattt gtcgaacaga taatgattta ccagattttc cagttgctcc
     1621 tggcgaccac tctgatgcac cggagacaaa ttatgttcct gagcatattt ggctaaatct
     1681 gccagtgaca tttggccttt caggatttgc tggcccaatt cgctattcca gccggaataa
     1741 cgctgcgcga tgcgtttatc cagctcgcca tcttcaatca tgcgcgctgc aattttcagc
     1801 gccagtgcca tcgtatccat cgcgccgata tgaccgtaaa acagatcata tttatcagta
     1861 ctttgacgac gtactttggc atcgaagttc agaccaccgg tggtgaaacc gcctgctttg
     1921 agaatttcat acatcaccag cgcattctct tccacactgt tcgggaactg gtcggtgtcc
     1981 cagcccagtt gcgcatcgcc acggttagcg tcgacagaac cgaacaggcc aagcgcaatg
     2041 gcggtggcta tttcatgatg gaaagagtga cctgccagcg tcgcgtggtt agcttcaatg
     2101 ttcagtttaa tctctttttc cagaccaaac tgtttcagga agccatagac cgtcgcggca
     2161 tcgtaatcat attgatgttt ggtcggttct tgcggtttcg gttcgataag caacgtgccc
     2221 tggaaaccga ttttatgttt atgctcaacc accatctgca taaagcggcc cagttgttca
     2281 cgctcctgac gcaagtcggt atttaacagc gtttcgtaac cttcacgacc gccccacagg
     2341 acatagtttt caccgcccaa tttatgggtt gcttccatcg ctgtaacaac ttgcgttgcc
     2401 gcccagctga agacttcagg atctgggttc gtcgccgcac ccgcgccgta gcgagggttt
     2461 gtaaagcagt tggcggttcc ccacagcagc ttcacgccgc tctcttcttg cttgcctgcc
     2521 agaacatcaa ccatttgcgc aaaattattg atgtactctt ttaacgacgc gccctcagga
     2581 gaaacatcca catcgtggaa gcaataaaat ggcacatgta acttgtggaa aaactcaaat
     2641 gcgacatctg ctttacgctt cgccaacgcc agtgcctcac caggctgctg ccacggacga
     2701 ttaaacgccc ccacaccaaa catatccgcc ccgttccagc agaaggtgtg ccagtagcag
     2761 gcggcaaatc gcaagtgctc ttccatacgc ttgcccaaca ccagttcgtc gggattgtag
     2821 tgacggaatg ctaacgggtt tgaggatttt gagccttcat aacgaacgcg atcgagctgg
     2881 tcaaaatagg cttgcatatt gaactccata atcaggtaat gccgcgggtg atggatgatg
     2941 tcgtaatatt gggcactccc tttcagttgc tcaattatgt tatttcacac tgctattgag
     3001 ataattcaca agtgtgcgct cgctcgcaaa ataaaatgga atgaggaaac tgggtaattc
     3061 ctcgaagaga aaaatgcaat aagtacaatt gcgcaacaaa agtaagatct cggtcataaa
     3121 tcaagaaata aaccaaaaat cgtaatcgaa agataaaaat ctgtaattgt tttcccctgt
     3181 ttagttgcta aaaattggtt acgtttatcg cggtgattgt tacttattaa agctgtcctc
     3241 taactacaga aggccctaca ccatgaaaat aaagaacatt ctactcaccc tttgcacctc
     3301 actcctgctt accaacgttg ctgcacacgc caaagaagtc aaaataggta tggcgattga
     3361 tgatctccgt cttgaacgct ggcaaaaaga tcgagatatt tttgtgaaaa aggcagaatc
     3421 tctcggcgcg aaagtatttg tacagtctgc aaatggcaat gaagaaacac aaatgtcgca
     3481 gattgaaaac atgataaacc ggggtgtcga tgtacttgtc attattccgt ataacggtca
     3541 ggtattaagt aacgttgtaa aagaagccaa acaagaaggc attaaagtat tagcttacga
     3601 ccgtatgatt aacgatgcgg atatcgattt ttatatttct ttcgataacg aaaaagtcgg
     3661 tgaactgcag gcaaaagccc tggtcgatat tgttccacaa ggtaattact tcctgatggg
     3721 cggctcgccg gtagataaca acgccaagct gttccgcgcc ggacaaatga aagtgttaaa
     3781 accttacgtt gattccggaa aaattaaagt cgttggtgac caatgggttg atggctggtt
     3841 accggaaaac gcattgaaaa ttatggaaaa cgcgctaacc gccaataata acaaaattga
     3901 tgctgtagtt gcctcaaacg atgccaccgc aggtggggca attcaggcat taagcgcgca
     3961 aggtttatca gggaaagtag caatttctgg ccaggatgcg gatctcgcag gtattaaacg
     4021 tattgctgcc ggtacgcaaa ctatgacggt atataaacct attacattgc tggcaaatac
     4081 tgccgcagaa attgccgttg aattgggcaa tggtcaggag ccaaaagcag atacctcact
     4141 gaataatggc ctgaaagatg tcccctcccg cctcctgaca ccgatcgatg tgaataaaaa
     4201 caacatcaaa gatacggtaa ttaaagacgg attccacaaa gagagcgagt tgtaagcgtt
     4261 acgccccagc gcggagcggg ggcgtgattt ctctccatgc cgcgtgaatg aattggctta
     4321 ggtggagtcg ttatgcctta tctacttgaa atgaagaaca ttaccaaaac cttcggcagt
     4381 gtgaaggcga ttgataacgt cagcttgcgg ttgaatgctg gcgaaatcgt ctcactctgt
     4441 ggggaaaatg ggtctggtaa atcaacgctg atgaaagtgc tgtgtggtat ttatccccat
     4501 ggctcctacg aaggcgaaat tatttttgcg ggagaagaga ttcaggcaag tcacatccgc
     4561 gataccgaac gcaaaggtat cgccatcatt caccaggaat tggccctggt gaaagaattg
     4621 accgtgctgg aaaatatctt cctgggtaac gaaataaccc acaatggcat tatggattat
     4681 gacctgatga cgctacgctg tcagaagctg ctcgcacagg tcagtttatc catttcacct
     4741 gatacccgcg ttggcgattt agggcttggg caacaacaac tggttgaaat tgccaaggca
     4801 cttaataaac aggtgcgctt gttaattctc gatgaaccga cagcctcatt aactgagcag
     4861 gaaacgtcgg ttttactgga tattattcgc gatctacaac agcacggtat cgcctgtatt
     4921 tatatttcgc acaaactcaa cgaagtcaaa gcgatttccg atacgatttg cgttattcgc
     4981 gacggacagc acattggtac gcgtgatgct gccggaatga gtgaagacga tattatcacc
     5041 atgatggtcg ggcgagagtt aaccgcgctt taccctaatg aaccacatac caccggagat
     5101 gaaatattac gtattgaaca tctgacggca tggcatccgg ttaatcgtca tattaaacga
     5161 gttaatgatg tctcgttttc cctgaaacgt ggcgaaatac tgggtattgc cggactcgtt
     5221 ggtgccggac gtaccgagac cattcagtgc ctgtttggcg tgtggcccgg acaatgggaa
     5281 ggaaaaattt atattgatgg caaacaggta gatattcgta actgtcagca agccatcgcc
     5341 caggggattg cgatggtccc cgaagacaga aagcgcgacg gcatcgttcc ggtaatggcg
     5401 gttggtaaaa atattaccct cgccgcactc aataaattta ccgatggcat tagccagctt
     5461 gatgacgcgg cagagcaaaa atgtattctg gaatcaatcc agcaactcaa agttaaaacg
     5521 tcgtcccccg accttgctat tggacgtttg agcggcggca atcagcaaaa agcgatcctc
     5581 gctcgctgtc tgttacttaa cccgcgcatt ctcattcttg atgaacccac caggggtatc
     5641 gatattggcg cgaaatacga gatctacaaa ttaattaacc agctcgtcca gcagggtatt
     5701 gccgttattg tcatctcttc tgaattacct gaagtgctcg gccttagcga tcgtgtactg
     5761 gtgatgcatg aagggaaact aaaagccaac ctgataaatc ataacctgac tcaggagcag
     5821 gtgatggaag ccgcattaag gagcgaacat catgtcgaaa agcaatccgt ctgaagtgaa
     5881 attggccgta ccgacatccg gtggcttctc cgggctgaaa gcactgaatt tgcaggtctt
     5941 cgtgatgatt gcagctatca tcgcaatcat gctgttcttt acctggacca ccgatggtgc
     6001 ctacttaagc gcccgtaacg tctccaacct gttacgccag accgcgatta ccggcatcct
     6061 cgcggtagga atggtgttcg tcataatttc tgctgaaatc gacctttccg tcggctcaat
     6121 gatggggctg ttaggtggcg tcgcggcgat ttgtgacgtc tggctaggct ggcctttacc
     6181 acttaccatc attgtgacgc tggttctggg actgcttctc ggtgcctgga acggatggtg
     6241 ggtcgcgtat cgtaaagttc cttcatttat tgtcaccctc gcgggcatgt tggcatttcg
     6301 cggcatactc attggcatca ccaacggcac gactgtatcc cccaccagcg ccgcgatgtc
     6361 acaaattggg caaagctatc tccccgccag caccggcttc atcattggcg cgcttggctt
     6421 aatggctttt gttggctggc aatggcgcag aagaatgcgc cgtcaggctt tgggtttgca
     6481 gtctccggcc tctaccgcag tagtcggtcg ccaggcttta accgctatca tcgtattagg
     6541 cgcaatctgg ctgttgaatg attaccgtgg cgttcccact cctgttctgc tgctgacgtt
     6601 gctgttactc ggcggaatgt ttatggcaac gcggacagca tttggacgac gcatttatgc
     6661 catcggcggc aatctggaag cagcacgtct ctccgggatt aacgttgaac gcaccaaact
     6721 tgccgtgttc gctattaacg gattaatggt agccatcgcc ggattaatcc tcagttctcg
     6781 acttggcgct ggttcacctt ctgcggggaa tatcgccgag ctggacgcaa ttgcagcatg
     6841 cgtgattggc ggcaccagtc tggcaggcgg tgtcgggagc gtggctggag cggtaatggg
     6901 ggcatttatc atggcttcac tggataacgg catgagtatg atggatgtac cgaccttctg
     6961 gcagtatatc gttaaaggtg cgattctgtt gctggcagta tggatggact ccgcaaccaa
     7021 acgccgttcc tgattttgat aaaaattttc tcaaagccgg taacgtatta ccggttttga
     7081 gtttttgcat gattcagcag gaaaagaacc atgtttacta aacgtcaccg catcacatta
     7141 ctgttcaatg ccaataaagc ctatgaccgg caggtagtag aaggcgtagg ggaatattta
     7201 caggcgtcac aatcggaatg ggatattttc attgaagaag atttccgcgc ccgcattgat
     7261 aaaatcaagg actggttagg agatggcgtc attgccgact tcgacgacaa acagatcgag
     7321 caagcgctgg ctgatgtcga cgtccccatt gttggggttg gcggctcgta tcaccttgca
     7381 gaaagttacc cacccgttca ttacattgcc accgataact atgcgctggt tgaaagcgca
     7441 tttttgcatt taaaagagaa aggcgttaac cgctttgctt tttatggtct tccggaatca
     7501 agcggcaaac gttgggccac tgaacgcgaa tatgcatttc gtcagcttgt cgccgaagaa
     7561 aagtatcgcg gagtggttta tcaggggtta gaaaccgcgc cagagaactg gcaacacgcg
     7621 caaaatcggc tggcagactg gctacaaacg ctgccaccgc aaaccgggat tattgccgtt
     7681 actgacgccc gggcgcggca tattctgcaa gtatgtgaac atctacacat tcccgtaccg
     7741 gaaaaattat gcgtgattgg catcgataac gaagaactga cccgctatct gtcgcgtgtc
     7801 gccctttctt cggtcgctca gggcgcgcgg caaatgggct atcaggcggc aaaactgttg
     7861 catcgattat tagataaaga agaaatgccg ctacagcgga ttttggtccc accagttcgc
     7921 gtcattgaac ggcgctcaac agattaccgc tcgctgaccg atccggccgt tattcaggcc
     7981 atgcattaca ttcgtaatca cgcctgtaaa gggattaaag tggatcaggt actggatgcg
     8041 gtgggcatat cacgctccaa tcttgagaag cgttttaaag aagaggtggg tgaaaccatc
     8101 catgccatga ttcatgccga gaagctggag aaagcgcgca gtctgctgat ttcaaccacc
     8161 ttgtcgatca atgagatatc gcaaatgtgc ggttatccat cgctgcaata tttctactct
     8221 gtttttaaaa aagcatatga cacgacgcca aaagagtatc gcgatgtaaa tagcgaggtt
     8281 ctgttgtagc gatgatgaga attgtcggcg tcgcatcagg taactcagca caaccacctg
     8341 atgcgagaaa aagccttatc cgaccgacaa gtcggataag gctcgaggaa atacaattac
     8401 atatgcgccg cgattaaccg ttggttatcc tggtacattg cgaacaggta gttgttataa
     8461 ctcttccctt tggtcgagta gcccttcagc ttgtgaatca tcgctgtggc agtcacttcc
     8521 tgatccgctt tacgcagctg tgcacgcgat ttacggaacg aagagtaagc cgggtgcgta
     8581 ttcaggttag tgacataggc gctcaccgat tctttgacag aactaaactg tgagtaccct
     8641 ttcactttac ctggcgcatt ggtacaacgt cctttcatgc atttcatgcc gaacagattg
     8701 ttattgttgc gcgccagctt cgacgttccc caaccgcttt ctgctgcagc catcgtcgcc
     8761 accatactgg tagggataat gtctacgcgt tcaagcaagg tattccacgg gatttttcgc
     8821 gtattaccag accacttcac cttgtagcgt ttggcgatgt ctttcagacg cgcacgctca
     8881 gcaggtgacc attggccctg atactgtttt gaaatcagcc agttacgttc tgcagtaatg
     8941 gctgcatttt ggctggtaat gtaaggcatt acggtccgga gaaacgcctt tttccttggt
     9001 gttccggaag ggtattttcg caaatcagga agtgaactac tctttgcact attgcgagaa
     9061 tactcttgtt tactgcttac ctgtttatta ctagctttag ttaagtggga cttttgactc
     9121 gctgttgttg tgtgcgtctt cgctaacacc tcactcgaaa acaccagagt gagtaacata
     9181 agaatcatcg ccccatatcg tcgtatggga gtcaaaatca tcaggtctcc tggtcggatt
     9241 taatcattcc aacaccttat atttttcaca aatttgagag ttgaatctca aatcatatca
     9301 aaaatagctg tcaagagcac cccaaggaat agtccaaatc tgaaactatg tcacgtgtta
     9361 acgattcaga ttggcgctaa atcgcagaaa atgtggggtt tatcgcaaaa ttcagccgtt
     9421 ttttgcgcga gatcgctcac ccttgcttct catcctgtgg acttaccgct cagggatgag
     9481 ttttgtttgg cttatcgctg gcaaactgtc tgaaatcgca gcaataagga ctcatccgcc
     9541 atgaaactcg ccgcctgttt tctgacactc cttcctggct tcgccgttgc cgccagctgg
     9601 acttctccgg ggttccctgc ctttagtgaa cagggaacgg gaacatttgt cagccacgcg
     9661 cagttgccca aaggtacgcg tccactaacg cttaattttg accaacagtg ctggcagcct
     9721 gcggatgcga taaaactcaa tcagatgctt tccctgcaac cttgtagcaa cacgccgcct
     9781 caatggcgat tgttcaggga cggcgaatat acgctgcaaa tagacacccg ctccggcacg
     9841 ccaacattga tgatttccat ccagaacgcc gccgaaccgg tagcaaacca ggtccgtgta
     9901 tgcccgaaat gggatggatt accgctcacg ctggatgtca gcgccacttt tccggaagga
     9961 gccgccgtac gggattatta cagccagcaa attgcgatag tgaagaacgg tcaaataacg
    10021 ttacaacccg ctgccaccag caacggttta ctcctgctgg aacgggcaga aactgacgtc
    10081 cctgcccctt tcgactggca taacgccacg gtttactttg tgctgacaga tcgtttcgaa
    10141 aacggcgatc ccagtaatga ccagagttac ggacgtcata aagacggtat ggcggaaata
    10201 ggtacttttc acggcggcga tttacgcggc ctgaccaaca aactggatta tctccagcag
    10261 ctgggcgtca atgctttgtg gataagcgcc ccatttgagc aaattcacgg ctgggtcggc
    10321 ggcggtacaa aaggtgattt cccgcattat gcctaccacg gttattacac acaggactgg
    10381 acgaatcttg atgccaatat gggcagcgaa gccgatctac ggacgctggt tgatagcgcg
    10441 catcagcgcg gtattcgtat tctctttgat gtcgtgatga accacactgg ctatgccacg
    10501 ctggcggata tgcaggagta tcagtttggc gcgttatatc tttctggtga cgagctgaaa
    10561 aaaacgctgg gtgaacgctg gagtgactgg aaacctgccg ccgggcaaac ctggcatagc
    10621 tttaacgatt acattaattt cagcgacaaa acaggctggg ataaatggtg gggaaaaaac
    10681 tggatcagaa cggatatcgg cgattacgac aatcctggat ttgacgatct cactatgtcg
    10741 ctggcctttt tgccggatat caaaaccgaa tcaactaccg cttctggtct gccggtgttc
    10801 tataaaaaca aaacggatac tcacgctaaa gtcatcgaag gctttacacc tcgcgattac
    10861 ttaacccact ggttaagtca gtgggtccgc gactatggga ttgatggttt tcgggtcgat
    10921 accgccaaac atgttgagtt gcccgcctgg cagcaactga aaaccgaagc cagcgccgcg
    10981 cttcgcgaat ggaaaaaagc taaccccgac aaagcattag atgacaaacc tttctggatg
    11041 accggtgaag cctggggcca cggcgtgatg caaagtgact actatcgcca cggcttcgat
    11101 gcgatgatca atttcgatta tcaggagcag gcggcgaaag cagtcgattg tctggcgcag
    11161 atggatacga cctggcagca aatggcggag aaattgcagg gtttcaacgt gttgagctac
    11221 ctctcgtcgc atgatacccg cctgttccgt gaagggggcg acaaagcagc agagttatta
    11281 ctattagcgc caggcgcggt acaaatcttt tatggcgatg aatcctcgcg tccgttcggt
    11341 cctaccggtt ctgatccgct gcaaggtaca cgttcggata tgaactggca ggatgttagc
    11401 ggtaaatctg ccgccaacgt cgcgcactgg cagaaaatca gccagttccg cgcccgccat
    11461 cccgcaattg gcgcgggcaa acaaacgaca ctttcgctga agcagggcta cggctttgtt
    11521 cgtgagcatg gcgacgataa agtgctggtc atctgggctg ggcaacagtg acttttccgg
    11581 cttcccgttc gtcagtacct cgggaagccg ccaaccagga taaaatgtca gccctaatca
    11641 gcgttgcagg ataaagcacc actcactctt caacagactg atttgcaccc cagcaaatgt
    11701 agcgttattg ttaccttcct tgctacagag ttcgacagat atcccgctat gacattctcc
    11761 ctttttggtg acaaatttac ccgccactcc ggcattacgc tgctgatgga agatctgaac
    11821 gacggtttac gcacgcctgg cgcgattatg ctcggcggcg gtaatccggc gcagatcccg
    11881 gaaatgcagg actacttcca gacgctactg accgacatgc tggaaagtgg caaagcgact
    11941 gatgcactgt gcaattacga cggtccacag gggaaaacgg agctactcac actgcttgcc
    12001 ggaatgctgc acgagaagtt gggttgggat atcgaaccac agaatattgc actaacaaac
    12061 ggcagccaga gcgcgttttt ctacttattt aatctatttg ccggacgccg tgccgatggt
    12121 cgggtcaaaa aagtgctgtt cccgctggca ccggaataca ttggctacgc tgacgccgga
    12181 ctggaagaag atctgtttgt ctctgcgcgt ccgaatattg aactgctgcc tgaaggccag
    12241 tttaaatacc acgtcgattt tgagcatctg catattggcg aagaaacggg gatgatttgc
    12301 gtctcccggc cgacgaatcc aacaggcaat gtgattactg acgaagagtt gctgaagctt
    12361 gacgcgctgg cgaatcaaca cggcattccg ctggtgattg ataacgctta tggcgtcccg
    12421 ttcccgggta tcatcttcag tgaagcgcgc ccgctatgga atccgaatat cgtgctgtgc
    12481 atgagtcttt ccaagctggg tctacctggc tcccgctgcg gcattatcat cgccaatgaa
    12541 aaaatcatca ccgccatcac caatatgaac ggcattatca gcctggcacc tggcggtatt
    12601 ggtccggcga tgatgtgtga aatgattaag cgtaacgatc tgctgcgcct gtctgaaaca
    12661 gtcatcaaac cgttttacta ccagcgtgtt caggaaacta tcgccatcat tcgccgctat
    12721 ttaccggaag atcgctgcct gattcataaa ccagaaggag ccattttcct ctggctatgg
    12781 tttaaggatt tgcccattac gaccgagcag ctctaccagc gcctgaaagc acgcggcgtg
    12841 ctgatggtgc cggggcacaa cttcttccca gggctggata aaccgtggcc gcatacgcat
    12901 caatgtatgc gcatgaacta cgtaccagag ccggagaaaa ttgaggcggg tgtgaagatt
    12961 ctggcggaag agatagaaag agcctgggct gaaagtcact aaaaattgcc tgatgcgcta
    13021 cgcttatcag gcctacatga tttctgcaat atattgaatt tggaagaatt tgtaggccgg
    13081 ataaggcgtt tacgccgcat ctggcataaa caaagcgcac tatccctgcg ttaaaaacgc
    13141 cggaaaaacg tttcaaacag gctgctgccg tagccgatgc cgctgcactt tctcgacatc
    13201 catgcactgc aacgcctttg tcgggcaggc ttcaacacag gccggtcccg tcgcccgatg
    13261 ccagcacaga tcgcacttaa tcgccctcgc ctttttgcgc gaagaaacga cctccatcgc
    13321 accaaacggg caagccagca tacagctttt acagccaatg caacgtgatt gttcaacgaa
    13381 aatatgccca tgttcgcggc ttatcgcgtc aacagggcag acattcgcgc acggtgcatc
    13441 ttcacactga tgacaggcta ctgccgtggt ccagctgtgg tctttaatga cacgaatacg
    13501 ggaaataaac tcgtctggtg acaacgcagc gcaatcctga ttctcctgat gcgacactgc
    13561 gcaagccact tcacaggtac ggcaaccgat acatttcgtc gcatccgcaa taataaaccg
    13621 gttcatgatc actcctttgc actaaaacag cagagtgcca gagagcgcgt ccctactgct
    13681 ttgatccaac gaaggaaatg ttaaagtttt ggcacttgtc caaagcgtta tgtgatcgcg
    13741 cccaggtcat ccctgacagt aaatcccagt tcattagaaa tagcctgcgc ggtttcacgc
    13801 agtggtttca ggagattttt ctcccccacc tgtttcagac gtgatgtcga aagcgaaatc
    13861 gacacggcac tacgaattga tatgttcctt gctgtaacgc cgcttccacg ctgctggcgt
    13921 taaaccggtg tgtttgcgaa aaatctgccg gaaatagcca acgtcattaa acccgcacaa
    13981 cgccgccact tccgtgagcg atcgcgcttc gctaattaac agcttttcgg cttcgcgcac
    14041 ccgctggcga tggattgctt ctgttaacgt cagatgaaac acccgccggt agacgcgccc
    14101 aagataatcg gcattgcagt acagctcgcg ggccagaagc gccgtagaaa gcgggcgatg
    14161 atattgcgtg cggatcagtt tctgcgcttt ccaggcaagg gtggtgcctg cgccatcggc
    14221 ctgttcatca agacgcgcgt cacgggtgag ctgctgcaaa atgagcagca caataaactc
    14281 cagcgccgga gtgcgttgat gcttttcctg ttcgcttaag aactggcgaa acagggccac
    14341 cagcgcctgc ggatcgacaa ccttcgtttg ttgcggcacg ctaagatgag taagccagcg
    14401 agggtcgttg tccggcgctt taacctcaaa atgcagccag taaaatttga gatcggcggg
    14461 aaaatcctcc accccaacat gttgtcggtt cggccagagt aataaactct ctcccgcatc
    14521 gaccagaaat atccgttctt cttcctgaat cgttaatcga cctttttcga caaagataat
    14581 ttcccatgac tccagccgcc ttgcaggatg cctccctacg ccacgggaaa taaataatcc
    14641 accattttgc actttaatcg gtaacgtcat ggataattcg agcattgata tgta
//
LOCUS       DAPGOF010000118        14478 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.119, whole genome shotgun sequence.
ACCESSION   DAPGOF010000118 DAPGOF010000000
VERSION     DAPGOF010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.119"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            52..525
                     /gene="trmL"
                     /locus_tag="RHV13_003220"
     CDS             52..525
                     /gene="trmL"
                     /locus_tag="RHV13_003220"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDV1242731.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYSAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(578..1399)
                     /gene="cysE"
                     /locus_tag="RHV13_003221"
     CDS             complement(578..1399)
                     /gene="cysE"
                     /locus_tag="RHV13_003221"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDV1242732.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(1479..2498)
                     /gene="gpsA"
                     /locus_tag="RHV13_003222"
     CDS             complement(1479..2498)
                     /gene="gpsA"
                     /locus_tag="RHV13_003222"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDV1242733.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(2498..2965)
                     /gene="secB"
                     /locus_tag="RHV13_003223"
     CDS             complement(2498..2965)
                     /gene="secB"
                     /locus_tag="RHV13_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HDV1242734.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASTQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(3028..3279)
                     /gene="grxC"
                     /locus_tag="RHV13_003224"
     CDS             complement(3028..3279)
                     /gene="grxC"
                     /locus_tag="RHV13_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HDV1242735.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(3421..3852)
                     /locus_tag="RHV13_003225"
     CDS             complement(3421..3852)
                     /locus_tag="RHV13_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDV1242736.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            4097..5641
                     /gene="gpmM"
                     /locus_tag="RHV13_003226"
                     /gene_synonym="pgmI"
     CDS             4097..5641
                     /gene="gpmM"
                     /locus_tag="RHV13_003226"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HDV1242737.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            5651..6934
                     /gene="envC"
                     /locus_tag="RHV13_003227"
     CDS             5651..6934
                     /gene="envC"
                     /locus_tag="RHV13_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HDV1242738.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            6938..7897
                     /locus_tag="RHV13_003228"
     CDS             6938..7897
                     /locus_tag="RHV13_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HDV1242739.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHTENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASAN"
     gene            complement(7884..8918)
                     /gene="waaH"
                     /locus_tag="RHV13_003229"
     CDS             complement(7884..8918)
                     /gene="waaH"
                     /locus_tag="RHV13_003229"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HDV1242740.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(9157..10182)
                     /gene="tdh"
                     /locus_tag="RHV13_003230"
     CDS             complement(9157..10182)
                     /gene="tdh"
                     /locus_tag="RHV13_003230"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HDV1242741.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(10192..11388)
                     /gene="kbl"
                     /locus_tag="RHV13_003231"
     CDS             complement(10192..11388)
                     /gene="kbl"
                     /locus_tag="RHV13_003231"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HDV1242742.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARNVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            11602..12534
                     /gene="rfaD"
                     /locus_tag="RHV13_003232"
     CDS             11602..12534
                     /gene="rfaD"
                     /locus_tag="RHV13_003232"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HDV1242743.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPLLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            12544..13590
                     /gene="rfaF"
                     /locus_tag="RHV13_003233"
     CDS             12544..13590
                     /gene="rfaF"
                     /locus_tag="RHV13_003233"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HDV1242744.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGVMRTAQDLPKPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            13594..>14478
                     /gene="rfaC"
                     /locus_tag="RHV13_003234"
     CDS             13594..>14478
                     /gene="rfaC"
                     /locus_tag="RHV13_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HDV1242745.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWKTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQM"
BASE COUNT         3603 a         3851 c         3747 g         3277 t
ORIGIN      
        1 cgcacggttc accaattttc tgctatcctg cccccgcact aagggggcag tatgctaaac
       61 atcgtacttt acgaaccaga aattccgcca aatactggca acatcatccg tctttgcgct
      121 aataccggct ttcgtctgca tatcatcgaa ccgatgggat ttgcctggga cgataagcgc
      181 ctgcgccgcg cggggctgga ctatcacgag tttaccgccg ttacgcgtca tcatgactac
      241 agcgcgttcc tcgaagcaga aaatccccag cgcctgtttg ccctcaccac gaaaggtacg
      301 cctgctcaca gcgccgtaag ctatcaggat ggcgattatc tgatgttcgg cccggaaaca
      361 cgcggcctgc cagcgagcat tcttgatgcc ctgcccgctg aacaaaaaat tcggattccg
      421 atggtgccag acagccgcag catgaacctg tccaatgcgg tgtcggtagt ggtgtatgaa
      481 gcctggcggc agctgggata tccgggagcg ttgttgagag attagtgacg ccatgccgga
      541 tagatgatta catcgcatcc ggcacgatca caggacatca gatcccatcc ccatactcaa
      601 atgtatggtt aataccgttg aaatgctggt ccatatccat tgatggctta tcgctgtctg
      661 gtttaccgac aatgcgtgcc ggaacgccgg cggcggtggt atgcggcggc accggttgca
      721 gcaccacgga acctgcgcca atcttcgcgc cgcgcccaac ttcaatattg ccgagaattt
      781 tcgcgcccgc gccaatcatc acaccttcac gaattttcgg gtgacggtca ccaccagatt
      841 tacccgtacc gccaagcgtc acagattgca gaatcgatac atcgttttca atcaccgccg
      901 tttcaccaac gacgatgcct gtcgcgtggt caagcatgat accgcgacca atttttgctg
      961 ccgggtgaat atcgacctgg aacgtcacag aaacctggtt ttgcagaaag attgccaatg
     1021 cgcgacgccc ctgattccac aaccagtgac cgatgcgata ggcctgcaag gcatgaaaac
     1081 ccttcaggta taacaacggg gttgagtatt tatccaccgc cgggtcgcgg gtacgcaccg
     1141 cctgaatatc acaggccgca gaggcgatca tttccgggtc ggcggcgtag gcttcttcca
     1201 ccacttcacg gatagcaata gcaggcataa ttggcgatga cagcttgttc gccagcatgt
     1261 agctcagtgc actgccaagg ttttcgtgct tgagtagcgt cgcgtggtaa aaactggcca
     1321 gcattggttc acagtccgcc agcgttctgg cttcggcttt aatattgttc cagacaattt
     1381 ccagttcttc acacgacatt gcttactcca cacgatgaga taatgaccgg cccgttctgc
     1441 gcgggccggg tcatagcggt aacaaaggtt ccctggggtt agtggctgct gcgctcgtcc
     1501 ttgcgtgcac gacctaataa cgtcaatgct gcctcgcgcg cgttttttcc gcaatataat
     1561 acttgataaa tttcctcggt tattggcatt tcaacgccga agcgatgcgc cagttcgcgg
     1621 acttctttcg tattgcggta gccttccacc acctgaccaa tcttctcctg cgcgctttgt
     1681 acatccatgc cctgaccgag catcatgcca aaacggcggt tacgcgactg gttgtcggta
     1741 caggtaagca ccagatcccc cagccccgcc atgcccataa aggtggcagg atcggcaccc
     1801 agcgccgcgc caagacgcga catttcagcc agcccacggg tgatcagcgc cgtacgcgca
     1861 ttcgcaccaa aaccgatacc gtcggacatc cccgcgccaa tggcaatgac gtttttcacc
     1921 gcgccgccaa gctgcacgcc aatgaaatcc gggttactgt aaacgcggaa acttttaccg
     1981 cagtgcagca gctgctggag atcatcggca aaggtctgat cggtcgaggc cagcgaaata
     2041 gccgtcggta aacctgccgc cagttctttc gcaaacgttg ggccagagat aaccgccagc
     2101 ggaatttgat cgcctaacgc ctcgcgggcc acgtcctgta acagacgccc ggtttccgct
     2161 tccagccctt tggtcgccca caccagacgc gcatcaggac gcatcagcgg tttaatctgg
     2221 cgcagcactt caccaaagac atggctgggt acgacgacga gaatattacg gctggctgcc
     2281 agcgcagtgg cgagatcgct ttcaagatgg agcgtatcgg gaaaaggcac atcggggaga
     2341 aacgcggcgt tacagcggtc gcgttcaagc gttgcgatat gttcagggtc atggccccag
     2401 aggacaacct cgtggccatt tcttgccagg gtgatggcaa gagcggtgcc gtacgagccg
     2461 gcaccgatca cagtcattga agcattacgt tggttcatca ggcatcctga tgttcttcag
     2521 taccttcgcc agcctgctgc tgcaaatagt tcatgaacag cgcatcgaag ttaaccggcg
     2581 caaggttcag ttgcgggaat gtaccgcggg ataccatgct ggtgatgcac tcacgagcat
     2641 acgggaacag aatgttcggg cagtatgcgc ccaggcaatg tgccatctgg gtgccttcga
     2701 tacccgcgat ggagaaaata ccgccctgct gaacttcaca caggaacgcg gtttcttcgc
     2761 ccagggaggc cgttacggta acacgcagta ccacttcgta aacgtcatct gccagttgag
     2821 tagatgccgt atccagatca agtttaactt ctggctgcca atctttctgg aaaacgtgcg
     2881 gcgcgttcgg cgcttcgaaa gaaatatcct tggtataaat acgttggatc tggaaagtca
     2941 tttcagtgtt gttttgttct gacatgtgta gaaaaccctt aagtgttgtc cttaaatact
     3001 gcgcaatgta atgccaacgt tcacacgtta tttcagcagg ggatccagtc caccacgtgc
     3061 atccaatgca tacaagtcat cacagccgcc aatgtgctgt gcgtcaataa aaatctgggg
     3121 aaccgtggtg cgaccgctgc gtttgatcat ctcttcacgc ttggcggcgt tgccatcgat
     3181 cggcaactcc tggaaactca cgcccttgct gctcagcagt gcttttgcac gatggcaata
     3241 cgggcaggtt tctttggtat agatttcaac attggccata aattagctcc tgttttttta
     3301 ccctgaaatt tcatgttgca gggcggcagc aaaatggagt gtccccagaa gcttataaca
     3361 ataagtcgct gggggcctaa ttgcagcaaa cggcctgcat catgaaagac gacaggtaaa
     3421 ttatttgccg cgcaccagag gcaagttttc gccagcccag ccagcaacgc cctctttcag
     3481 cacgaatact tgcgcaaaac cagctttcgt cagcgcgttt gcaggctcct ggcattgcat
     3541 accagaaccg tctaccacga taaccggttt gtctttgtgc ttctcaagct caccaacatt
     3601 gttggctttg atttcgctcg gcaacagatt gatagaacct gcgatatggc ctttacggaa
     3661 gtcatcacgc tgacgtaaat ccacaaccac agcgtcttct ttgttgatca gacgcgtagc
     3721 ttcaccacga gtaatcacct tcactttcga ggtcaggctc ttaaacgtag tcacaagaac
     3781 cgccaccagt aacgcgatcc aggcgatact cagtatggga tgacggccaa caaattgcat
     3841 aatttcttgc atggggggta acaactcccg acgtagtgat taaaaaaacc aggaaaggag
     3901 tatacctgcg cggtgcggca aatacagcca gcgcgttaac tggaatgcaa ttttgcgggg
     3961 cacgacgaaa aaaaagcgta aatctggcat cggcttgccg cgagcggtcg tatattttga
     4021 tcttcagagg ctattttatc gattcagctg tagtaaaatt acgcaaattt tgactcttga
     4081 gtatgaggtt gtcgcaatgt cggtttctaa aaaacctatg gtactggtga ttctggatgg
     4141 ctatggctat cgcgaagaac agcaggataa cgccattttt agtgctaaaa ccccggtaat
     4201 ggatgcactg tgggccaatc gtccgcatac cctgatcgac gcttccggtc tggaagtcgg
     4261 tctgcctgac cgtcagatgg gtaactccga agtaggccac gttaacctgg gtgccggccg
     4321 catcgtgtat caggacctga ctcgtctgga cgttgaaatc aaagatcgcg ctttctttgc
     4381 taatccggtg ctgactggcg cggtagataa agcgaaaaac gcaggcaaag cggtacacat
     4441 tatgggtctg ctctccgcag gcggcgtaca cagccacgaa gatcacatca tggcgatggt
     4501 agaactggca gctgaacgcg gcgcagaaaa aatctacctg cacgcattcc ttgacggtcg
     4561 cgacactccg ccgcgcagtg ctgaatcctc gctgaaaaaa ttcgaagaga aatttgccgc
     4621 gctgggtaaa ggccgcgtag cgtccatcat tggtcgttac tacgcgatgg accgcgataa
     4681 ccgttgggat cgcgttgaaa aagcctatga cctgctgact ctggcgcagg gtgagttcca
     4741 ggccgatact gccgttgctg gtttgcaggc agcttatgct cgcgacgaaa acgacgagtt
     4801 cgtgaaagcg accgttattc gtgcagaagg tcagccagat gcggcaatgg aagacggcga
     4861 tgcgctgatt ttcatgaact tccgtgctga ccgcgcgcgt gaaatcactc gtgcattcgt
     4921 gaacgctgat ttcgatggct tcgcgcgtaa gaaagtggtt aacgtcgatt tcgtgatgct
     4981 gaccgaatac gccgctgaca tcaaaactgc ggttgcttac ccacccgctt ccctggttaa
     5041 caccttcggc gagtggatgg cgaaaaacga caaaactcag ttgcgtattt ccgaaaccga
     5101 aaaatatgcc cacgttactt tcttcttcaa cggtggcgta gaagagtcgt tcaaaggcga
     5161 agatcgcatt ctgatcaact caccgaaagt ggctacctac gatctgcaac cggaaatgag
     5221 ctccgcagag ctgaccgaaa aactggttgc ggccatcaag agcggcaaat acgacaccat
     5281 tatctgtaac tatccgaacg gcgacatggt aggtcacacc ggggtgatgg aagcggcggt
     5341 taaagcggtt gaagcactgg atcactgcgt ggaagaagtc gcgaaagcgg ttgagtccgt
     5401 gggtggacaa ctgctgatca ccgctgacca cggtaacgct gaacagatgc gcgatccggc
     5461 aacgggtcag gcacacacgg cacacaccaa cctgccagtt ccgctgattt acgttggtga
     5521 taagaacgtg aaagcggttg caggcggcaa actttctgac atcgcgccga ccatgttgtc
     5581 gctgatgggt atggaaatcc cgcaagagat gactggtaag ccgctgttca tcgtggaata
     5641 atccctcccc atgaggggaa aggcgattaa taccatgaca cgggccgtga aaccgcgcag
     5701 gtttgcaatc aggcccatca tctacgccag cgttcttagc gctggcgtat tgttgtgcgc
     5761 cttttccgcc cacgcggatg agcgtgacca actcaaatct attcaggccg atatcgccgc
     5821 aaaagagcgc gcggtacgcc aaaagcaaca acaacgcgca agcctgctcg cacaattgaa
     5881 aaagcaggaa gaagcgatct ctgaagccac ccgtaagctg cgcgaaacgc aaaacacgct
     5941 caatcaactc aataaacaga ttgatgagat gaacgcgtcg attgccaaac tggagcagca
     6001 aaaagccgcc caggagcgca gcctcgccgc acaactggat gccgcattcc gtcagggcga
     6061 gcataccggt attcagctga ttctcagcgg tgaagaaagc cagcgtggac agcgtttaca
     6121 ggcttatttc ggctatctca accaagcgcg acaagaaacc attgcccagt tgaagcaaac
     6181 gcgtgaagaa gtcgccatgc agcgtgctga actggaagag aaacagagcg agcaacaaac
     6241 gctgttatat gagcagcgcg cccaacaggc gaaactgact caggcgctga acgagcgtaa
     6301 aaagacgctg gcagggctgg aatcttccat ccagcaaggt cagcaacagt tgagcgagct
     6361 gcgcgccaac gaatcccgtc tgcgtaacag cattgcccgt gcggaagccg cggcgaaagc
     6421 gcgtgcagaa cgagaagcgc gtgaggccca ggcggttcgc gaccgccaga aagaagcgac
     6481 gcgcaaaggc accacctaca aaccgaccga aagcgaaaaa tcgctgatgt cccgaactgg
     6541 tggcctgggt gcgccgcgtg gtcaggcatt ctggccagtt cgtggcccta cgctgcatcg
     6601 ctatggcgag cagctacagg gtgaattacg ctggaaagga atggtgatcg gtgcctctga
     6661 aggtactgaa gttaaagcga ttgccgacgg tcgggtgatt ctggctgact ggctgcaagg
     6721 ttatggcttg gtggtggtgg ttgagcatgg taaaggcgac atgagtcttt acggctataa
     6781 tcagagcgcg ctggtgagcg ttggttcgca ggttcgcgcg ggccagccga ttgcactggt
     6841 gggcagcagt ggcggtcagg gtcggccttc actctatttc gaaattcgcc gccagggtca
     6901 ggcggtcaat ccacagccgt ggttgggaag ataagttttg tttccatttc gtcgtaacgt
     6961 tcttgcattt gccgctctgt tggcgctctc ctcccccgta cttgctggca aacttgccat
     7021 cgtcattgat gattttgggt atcgcccgca caccgaaaac caggtgctgg cgatgccttc
     7081 cgctatctcc gtcgctgtat tacccgattc accgcacgcc agagagatgg cgaccaaagc
     7141 gcataacagc ggtcacgaag tgttgattca tctaccaatg gcaccgttga gtaaacagcc
     7201 gctggagaaa aatacgctgc gcccggagat gagcagcgac gaaattgagc gcattattcg
     7261 tagtgcggtc aataacgtgc cctatgccgt ggggatcaac aaccacatgg gcagcaagat
     7321 gacctctaac ctgtttggta tgcagaaagt gatgcaggcg ctggagcgtt acaatcttta
     7381 cttcctcgac agcgtaacca ttggtaatac ccaggcgatg cgcgccgcgc aaggcactgg
     7441 cgtgaaggtt attaaacgga aggtgttcct cgacgattca cagaacgaag cggacatccg
     7501 tgtgcagttc aatcgcgcaa ttgatctggc gcgtcgcaac ggttcaacca ttgccattgg
     7561 gcatcctcac ccttcaacgg tacgcgtgct acaacagatg gtttataacc tgccgccaga
     7621 cattacgctg gtgaaagcca gcagcttgct gaatgaaccg caggttgata cgtctacacc
     7681 gccgaaaaac actgtgcccg acacaccgcg taatccgttc cgtggcgtga agctgtgcaa
     7741 accgaagaaa ccgatagaac ctgtttatgc taatcgcttc tttgaagtat taagcgaaag
     7801 catcagccag agcacgctga tcgtttactt ccagcatcag tggcaaggct ggggcaaaca
     7861 gcccgaagcg gcgaaactta acgctagcgc aaattaaagg cgctacgcgt aatgcgatgg
     7921 tgcgaccgcg ttttgtcgcg ccattgccat aagcggaaag accacagtaa cgcctggtaa
     7981 cccaccttca cgctgcgcac attggtaatc aggcgcttat acataccgga agtaaatatc
     8041 tcggcaatca tccgttggcg ggtaagaata tccggctctt tgcgcaccgc atggcaaaca
     8101 cgcaatgctt cgtaagttat ttgctgatga aattccggat aaatcgtaat tttgtcggca
     8161 taatttcgat ttaatttctc cagcaggcgg gtaatcttaa tatagtgacg ttgataatta
     8221 aggtttttat tcccttgtct atgcaaccga ctcactgacg tattatgcag ataatattta
     8281 tataatgatt gctcggtata tcgcgcacgc agcgcattaa acatgaattc tgttgtccag
     8341 acaatatcct gatgatgtaa tccggcaata aatttaatgt tatttttaac aataacatcg
     8401 cggcgataaa cccccatcca gacaacgtga gtccaacggc gcgaagaaag ccccatccgc
     8461 agccagtccg ggccggttaa tacaccggtt gagcgaaggc gatcgctggg gatggattgc
     8521 caggtttctc ccgtttcacg aaaacaccag tcagcgttgc actgcgccac gtcgaggtcg
     8581 tcctctaacg ccatggtcat cagcgtttcg tacatggtgg gatagacttc atcgtcagca
     8641 tcgacaaaag cgacatattt gcccgttgcc acttcaatcc cacgattacg cgccaccgat
     8701 gcgccagcat tcgcctgatg caacaaacga acgtgcggat agttttctgc gtaatgcttt
     8761 gctatttcaa cagaattatc cgttgaacca tcgttaataa taatgatttc cagagcagtc
     8821 caggtttgcg taattaaaga ttccatacaa gtgcggaaat catcgcccgc attatataac
     8881 ggaataataa cactaagttt attggtgctg ttcatcataa attaccgacg acctctgaag
     8941 ttatcctgac gttttacagg ctttgtatta agaaactatt aagcctgtga gatattattt
     9001 ttacaggtga gagagtcact tcagggtaga gagataatga gagcagctat ataagagatc
     9061 agcacaatta cgaactcttt attacactga caggatttta catatttaac agattatcct
     9121 cagataaaag ggctggaata ccagcccttg ttcgtgttaa tcccagctca gaataacttt
     9181 cccggactgg cccgaacgca tagcgtcaaa gcccttctgg aaatcatcga tagagaaacg
     9241 atgggtaatg atcggcgaga gatcgaggcc agactgaatc agcgccgcca tcttgtacca
     9301 ggtttcaaac atctcacgac cgtaaatgcc tttaatgaac aagcccttaa agatcacttt
     9361 tgtccagtct atcgacatgt cagaaggtgg aatacccagc atcgcaatac gaccgccgtg
     9421 gttcatggtg tcgagcatgg tacgaaacgc tggcggcgca ccggacattt ccagaccgac
     9481 atcaaagcct tcggtcatgc ccagttctgc catcacatca ttaaggtttt ctttagcgac
     9541 gttaaccgca cgggtgatac ccattttgcg cgccagttcg agacgatatt cattaacatc
     9601 agtgatcacc acattgcgtg caccaacgtg tttcgccacc gccgcagcca taatacctat
     9661 cggacctgca ccagagacca gcacatcctc gccaaccaga tcgaacgaca gcgcggtatg
     9721 cacggcgtta ccgaagggat caaaaatgga agccaggtcg tcggagatat tgtctgggat
     9781 tttgaaggcg ttgaacgccg ggatcaccag atattcggcg aagcagcccg gacggttaac
     9841 gccgacgccg atcgtgttgc ggcacagatg ggtacgaccg ccacgacagt tgcgacagtg
     9901 accgcaagtg atatggcctt cgccagaaac gcgatcgcca atcttgaagc ctttcacttc
     9961 ctgaccaata cctaccactt caccgacata ttcatggccc acgaccatcg gcaccgggat
    10021 ggttttttgc gaccactcat cccagttata gatgtgaacg tcagtcccgc agatggctgt
    10081 tttacggatt ttaatcagca gatcgttatg cccgagttcc ggtacaggaa cgtcggtcat
    10141 ccagatgccc tcttccgctt tcagtttgga taacgctttc atctcacatc ctcaggcaat
    10201 aacgcccagt tgtttaccaa tacgcgtgaa tgcttctact gcacgcgtaa tttgctcagg
    10261 ggtatgcgcc gcagacatct gggtacgaat acgcgcctga cctttcggaa cgaccggata
    10321 gaagaaaccg gtgacgtaaa tgccctcttt ttgcagctca cgggcaaatt tctgcgccac
    10381 taccgcatca ccaagcatga ccggaataat ggcgtgatcg gctcccgcca gggtaaagcc
    10441 cgctgccgac atttgctcac ggaactgacg cgcgttcgcc cacagacgat cacgcagctc
    10501 gctacccgct tcgaccatct ccagcacttt gatggatgcg gcaacaatgg ccggtgccag
    10561 cgagttggag aacagatacg gacgagaacg ctggcgcagc cactcaacca cttctttgcg
    10621 cgccgcggta taaccaccag aagccccgcc cagcgcttta ccaagcgtac cggtgataat
    10681 atcgacccgg cccatcacat cgcagtattc atgggaacca cgaccatttt caccgacaaa
    10741 accgaccgcg tgggagtcgt ctaccatcac cagggcatcg tatttatctg ccagatcgca
    10801 aacgcctttc aggttggcaa tcacgccgtc catcgagaac acaccatcgg tggcaatcag
    10861 cacattacgc gcaccggctt cacgcgcttc tttcagacga gcttcaagct cctgcatatc
    10921 gttgttggca tagcgatagc gtttagcttt acacagacgc acaccatcaa taatagacgc
    10981 gtggttcagt gcgtcggaga taatggcgtc ttccgcgccc agcagcgttt caaacaggcc
    11041 accgttagca tcaaagcagg aagagtagag aatcgcatct tccatcccca ggaatgccgc
    11101 cagtttttgt tcaagctctt tatggctgtc ctgagtgccg caaataaaac gcaccgaagc
    11161 catgccgaaa ccgtgagaat ccattcccgc ctttgctgcc gcaatcagat ccggatgatt
    11221 cgccagcccg agatagttgt tggcacaaaa gttaatgacg tggcttccat cagccacggt
    11281 gatatctgct tgctgcgcag acgtaataat gcgctcttct ttaaacaacc cttccgcccg
    11341 tgcggtttcc agatcgttgg ttaactgctg ataaaattct ccacgcattg cgattctcca
    11401 gacagggcaa attccagcac atattaccca aacttatagg tcgcgacgag atgacgcgtt
    11461 aacacttctg caaaattcag gataaataac gtccatcaat ctcacttttg gcgaatggct
    11521 gaagccaacg ccgtgtagtg atatgataga agtattcgtg tctgagattg tctctgactc
    11581 cataattcga aggttacagt tatgatcatc gttaccggcg gcgcgggctt tatcggcagc
    11641 aacatcgtta aggccctgaa tgataaaggc atcaccgata ttctggtggt ggacaacctg
    11701 aaagacggca ccaagtttgt gaacctggtg gatctggata tcgcggacta tatggataag
    11761 gaagacttcc tgatccagat tatggctggc gaagagttcg gcgatgtcga agcgattttc
    11821 cacgaaggcg catgctcttc caccaccgag tgggatggca agtatatgat ggataacaac
    11881 tatcaatact ccaaagagct gctgcactac tgtctggagc gcgaaatccc gctcctgtat
    11941 gcatcttccg cggccaccta cggcggacgc acctccgact ttattgaatc ccgcgagtac
    12001 gaaaaaccgt tgaacgtcta cggttactca aaattcctgt ttgatgaata tgttcgtcaa
    12061 atcctgcctg aagcgaactc gcagattgtt ggattccgtt atttcaacgt ttatggaccg
    12121 cgtgaaggcc ataaaggcag catggcgagc gtcgctttcc atctcaacac ccagcttaac
    12181 aacggtgaat caccgaagct gtttgaaggt agcgagaact tcaaacgtga cttcgtctat
    12241 gtgggcgacg tggcagatgt aaacctgtgg ttcctggaaa atggcgtttc cggcatcttc
    12301 aacctcggca ccggtcgtgc ggaatccttc caggctgtag ctgatgctac gctggcttat
    12361 cacaagaaag gccagatcga atacattccg ttcccggata agctgaaagg ccgctaccag
    12421 gcgttcactc aggcagatct gacaaatctg cgcgcggcgg gttacgacaa accgttcaaa
    12481 accgttgctg aaggtgtaac ggaatacatg gcctggctga atcgcgacgc ataagagctc
    12541 tgcatgaaaa tactggtgat cggcccgtct tgggttggcg acatgatgat gtcgcaaagt
    12601 ctctatcgca cgctccaggc gcgctatccc caggcgataa tcgatgtgat ggcaccggca
    12661 tggtgccgtc cattattatc gcggatgccg gaagttaacg aagctattcc tatgcctctc
    12721 ggtcacggag cgctggaaat cggcgaacgc cgcaaactgg gtcatagcct gcgtgaaaag
    12781 cgctacgacc gcgcctacgt cttacccaac tccttcaaat ctgcattagt gcctttcttc
    12841 gcgggtattc ctcatcgcac cggctggcgc ggcgagatgc gctacggttt actcaacgat
    12901 gtacgcgtgc tcgataaaga agcctggccg ctaatggtgg aacgctatgt cgcgctggcc
    12961 tatgacaaag gcgtgatgcg taccgcacaa gatctaccaa aaccgttgtt atggccgcaa
    13021 ttgcaggtga gcgaaggtga aaaatcatat acctgtaatc aattttcgct ttcatcagaa
    13081 cgtccgatga ttggtttttg cccgggtgcg gagtttggtc cggcaaaacg ctggccacac
    13141 taccactatg cggagctggc aaagcagctg attgatgaag gttatcaggt ggttctgttt
    13201 ggctctgcga aagatcatga agcgggcaat gagattcttg ccgctttgaa taccgagcag
    13261 caggcatggt gtcggaacct ggcgggggaa acacagcttg atcaagcggt tatcctgatt
    13321 gcagcctgta aagccattgt cactaacgat tctggcctga tgcatgttgc ggcggcgctc
    13381 aatcgtccgc tggttgccct gtatggtccg agtagcccgg acttcacacc gccgctatcc
    13441 cataaagcgc gcgtgatccg tttgattacc ggctatcaca aagtgcgtaa aggtgacgct
    13501 gcggagggtt atcaccagag cttaatcgac attactcccc agcgcgtact ggaagaactc
    13561 aacgcgctat tgttacaaga ggaagcctga cggatgcggg ttttgatcgt taaaacatcg
    13621 tcgatgggcg atgttctcca tacgttgccc gcactcactg atgcccagca ggcaatccca
    13681 gggattaagt ttgactgggt ggtggaagaa gggttcgcac agattccttc ctggcacgct
    13741 gccgttgagc gagttattcc tgtggcaata cgtcgctggc gtaaagcctg gttctcggcc
    13801 cccataaaag cggaacgcaa agcgtttcgt gaagcgctac aagcagagaa ctatgacgca
    13861 gttatcgacg ctcaggggct ggtaaaaagc gcggcgctgg tgacgcgtct ggcgcatggc
    13921 gtaaagcatg gcatggactg gaaaaccgct cgcgaacctt tagccagtct gttttacaat
    13981 cgtaagcatc atattgcaaa acagcagcac gccgtagaac gcacccgcga actgtttgcc
    14041 aaaagtctgg gctatagcaa accgcaaacc cagggcgatt atgctattgc acagcatttc
    14101 ctgacgaacc tgcctacaga tgctggcgaa tatgccgtat ttcttcatgc aacgacccgt
    14161 gatgataaac actggccgga agaacactgg cgagaattga ttggtttact ggctgattca
    14221 ggaatacgga ttaaacttcc gtggggcgcg ccgcatgagg aagaacgggc gaaacgactg
    14281 gcggaaggat ttgcttatgt tgaagtattg ccgaagatga gtctggaagg cgttgcccgc
    14341 gtgctggccg gggctaagtt tgtggtttca gtggatacgg ggttaagcca tttaacggcg
    14401 gcactggata gacccaatat cacggtttat ggacctaccg accctggatt aattggcggg
    14461 tatgggaaga atcagatg
//
LOCUS       DAPGOF010000119        14448 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.120, whole genome shotgun sequence.
ACCESSION   DAPGOF010000119 DAPGOF010000000
VERSION     DAPGOF010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.120"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..294
                     /locus_tag="RHV13_003235"
     CDS             <1..294
                     /locus_tag="RHV13_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDV1242746.1"
                     /translation="RPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGG
                     EATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            450..1985
                     /gene="gadC"
                     /locus_tag="RHV13_003236"
     CDS             450..1985
                     /gene="gadC"
                     /locus_tag="RHV13_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HDV1242747.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2116..3435
                     /locus_tag="RHV13_003237"
     CDS             2116..3435
                     /locus_tag="RHV13_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HDV1242748.1"
                     /translation="MDICSRNEKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIT
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3807..5189
                     /gene="dosC"
                     /locus_tag="RHV13_003238"
     CDS             3807..5189
                     /gene="dosC"
                     /locus_tag="RHV13_003238"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HDV1242749.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            5214..7613
                     /gene="dosP"
                     /locus_tag="RHV13_003239"
                     /gene_synonym="pdeO"
     CDS             5214..7613
                     /gene="dosP"
                     /locus_tag="RHV13_003239"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HDV1242750.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            7871..8452
                     /gene="ddpX"
                     /locus_tag="RHV13_003240"
     CDS             7871..8452
                     /gene="ddpX"
                     /locus_tag="RHV13_003240"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HDV1242751.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHVS"
     gene            8466..10016
                     /locus_tag="RHV13_003241"
     CDS             8466..10016
                     /locus_tag="RHV13_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDV1242752.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTAVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDTPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYS
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQVDLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            10018..11040
                     /locus_tag="RHV13_003242"
     CDS             10018..11040
                     /locus_tag="RHV13_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV1242753.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGCPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYDH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            11037..11933
                     /locus_tag="RHV13_003243"
     CDS             11037..11933
                     /locus_tag="RHV13_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HDV1242754.1"
                     /translation="MMLSEETSAVRPAKQTRFNGAILVWMLKGSPLTVTGAVIIVLML
                     LMLIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            11930..12916
                     /locus_tag="RHV13_003244"
     CDS             11930..12916
                     /locus_tag="RHV13_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV1242755.1"
                     /translation="MTQPVLDIQQLHLSFPGFKGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRERCYATGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            12909..13835
                     /locus_tag="RHV13_003245"
     CDS             12909..13835
                     /locus_tag="RHV13_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV1242756.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKHSSEQQRRALAEELAMQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTAPSHPYTRLLLDSLPAIDKPLEEEWALRKTELPGNRTLPQGCFF
                     RERCPLATSGCEVRQSLAIREDGRELRCWRAL"
     gene            complement(13891..14322)
                     /gene="osmC"
                     /locus_tag="RHV13_003246"
     CDS             complement(13891..14322)
                     /gene="osmC"
                     /locus_tag="RHV13_003246"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HDV1242757.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         3529 a         3599 c         3688 g         3632 t
ORIGIN      
        1 cgcccggacg aaggcatccc ggcggtttgc ttcaaactga aagatggtga agatccggga
       61 tacaccctgt acgacctctc tgaacgtctg cgtctgcgcg gctggcaggt tccggccttc
      121 actctcggcg gtgaagccac cgacatcgtg gtgatgcgca ttatgtgtcg tcgcggcttc
      181 gaaatggact ttgctgaact gttgctggaa gactacaaag cctccctgaa atatctcagc
      241 gatcacccga aactgcaggg tattgcccaa cagaacagct ttaaacatac ctgataacgt
      301 ttaacggtaa cggtgtcccg aaacgaaccc gtttcgggac aatttccaaa gtctgttcac
      361 tggcattagc aacggaaaat attgttctga atacgcttca gaacaaaact ggtgcggttc
      421 cgacaggaat accgttttag ggggataata tggctacatc agtacagaca ggtaaagcta
      481 agcagctcac attactcggc ttttttgcca taacggcatc gatggtaatg gctgtttatg
      541 aataccctac cttcgcaaca tcgggctttt cattagtctt cttcctgcta ttaggcggga
      601 ttttatggtt tattcccgtg ggactttgtg ctgcggaaat ggccaccgtc gacggctggg
      661 aagaaggtgg tgtcttcgcc tgggtatcaa atactctggg gccgagatgg ggatttgcag
      721 cgatctcatt tggctatctg caaatcgcca ttggttttat tccgatgctc tatttcgtgt
      781 taggggcact ctcctacatt ctgaaatggc cagcgctgaa tgaagacccc attaccaaaa
      841 ctattgcagc actcatcatt ctttgggcgc tggcattaac gcagtttggt ggcacgaaat
      901 acacggcgcg aattgctaaa gttggcttct tcgccggtat cctgttacct gcatttattt
      961 tgattgcatt agcggctatt tatctgcact ccggtgcccc cgttgctatc gaaatggatt
     1021 cgaagacctt cttccctgac ttctctaaag tgggcaccct ggttgtattt gttgccttca
     1081 ttttgagtta tatgggcgta gaagcttccg caacccacgt caatgaaatg agtaacccag
     1141 ggcgcgacta tccactggct atgttactgc tgatggtggc ggcaatctgc ttaagctctg
     1201 ttggcggttt gtctattgcg atggtcattc cgggtaatga aatcaacctc tccgcagggg
     1261 taatgcaaac ctttaccgtt ctgatgtccc atgtggcacc ggaaattgag tggacggttc
     1321 gcgtgatctc cgcactgctg ttgctgggtg ttctggcgga aatcgcctcc tggattgttg
     1381 gtccttctcg cgggatgtat gtcacagcgc agaaaaacct gctgccagcg gcattcgcta
     1441 aaatgaacaa aaatggcgta ccggtaacgc tggtcatttc acagctggtg attacttcta
     1501 tcgcgttgat catcctcacc aataccggtg gcggtaacaa catgtccttc ctgatcgcac
     1561 tggcgctgac ggtggtgatt tatctgtgtg cttatttcat gctgtttatt ggctacattg
     1621 tgttggttct taaacatcct gacttaaaac gcacatttaa tatccctggt ggtaaagggg
     1681 tgaaactggt cgtggctatt gtcggtctgc tgacttcaat tatggcgttt attgtttcct
     1741 tcctgccgcc ggataacatc cagggtgatt ctaccgatat gtatgttgaa ttactggtcg
     1801 ttagtttcct ggtggtactt gccctgccct ttattctcta tgctgttcat gatcgtaaag
     1861 gcaaagcgaa taccggcgtc actctggagc caatcaacag tcagaacgca ccaaaaggtc
     1921 acttcttcct gcacccgcgt gcacgttcac cacactatat tgtgatgaat gacaagaaac
     1981 actaaccatg tctgaatggt tataagacaa gggagcgata attcatcgct ccctttttcg
     2041 tgcttgctgt cgtattgact taaccgggat aagtacgaga atgagcgcac atctgtttac
     2101 cggaaaccag cacatatgga tatctgctcc cgaaacgaga aattaacgat tagaagacca
     2161 gcgatactgg ttgcactggc acttttactg tgtagttgta aaagcacgcc tccagagtcc
     2221 atggtgacac caccagcggg ttcaaagcca ccagccacga cgcaacaatc gtcacaaccg
     2281 atgcgtggca tctggctggc cacggtgtct cgcctcgact ggccaccggt ttcctcggtt
     2341 aacattagta accccaccag ccgggcccgt gtacaacaac aggcgatgat cgacaaactg
     2401 gatcatctgc aacgtctcgg cataaacacg gtctttttcc aggtcaagcc ggacggtacc
     2461 gccctgtggc catcgaaaat tttgccgtgg tccgatctta tgaccggtaa gattggtgaa
     2521 aatccgggtt acgatccgct gcaatttatg ctcgacgaag cccacaagcg tgggatgaaa
     2581 gtacacgcct ggtttaaccc ctatcgcgta tcggttaata cgaagcccgg tactatcagg
     2641 gaactgaata gcactctgtc tcaacaaccg gcgagcgtct atgtgcaaca ccgcgactgg
     2701 atcagaacgt ctggcgatcg ctttgtcctc gacccgggca tccctgaggt tcaggactgg
     2761 atcacatcaa tagtcgcaga agtggtttcc cgctatcccg tagatggcgt gcagtttgac
     2821 gactatttct atacggagtc accgggttca cggctaaatg ataacgaaac gtaccgtaaa
     2881 tacggaggcg catttgcgtc aaaagcagac tggcggcgca acaatactca gcagttaatt
     2941 acaaaagtat cgcacaccat taaaagcatt aagccgggag tcgaatttgg cgttagcccg
     3001 gcaggcgtgt ggcgtaaccg atcacacgat ccgctcggtt ccgatacccg aggcgcggca
     3061 gcctatgacg aatcctacgc tgacacccgt cgatgggtgg aacaaggatt gctggattac
     3121 attgctcccc aaatttactg gccgttctca cggagtgccg cgcgttatga cgtgttggca
     3181 aaatggtggg cggatgtcgt taaaccgacc aggacccgcc tgtatatcgg tatcgccttc
     3241 tataaagtgg gtgaaccctc aaagatagag ccagactgga tgattaacgg cggcgtaccg
     3301 gaactgaaaa agcagctcga tcttaacgat gcggtaccag aaattagcgg caccatcttg
     3361 ttccgtgagg actatctgaa taaaccacag actcaacaag cggtcagcta tctgcaaagt
     3421 cgttggggca gttaaactcc catgcttact ttgtaacaag ccggtgcttt acccaccggc
     3481 ttgttacacc ttgtgaaata tcccactagt aatcatgctt acataagtca aattaaccac
     3541 gcaattaacc acacttagtt aaacagctat aaatccctaa taaaaatgta tgaatatgaa
     3601 aaaccatacg atattcgctg tcaccgaaaa ataattacga tgggctatac tcttctgaag
     3661 aacagtagcg tagattgaaa ctgtcctaag tcgcttattt cttttcagta tatcttcata
     3721 tttcaggaga atatggtgca gttagtcgat ctcgcacgtt gcgttttgat tccgtctgat
     3781 ttcccagcct tataagggtg tgaattatgg agatgtattt taaaagaatg aaagatgagt
     3841 ggaccggact tgtcgaacag gcagatccac tcattcgtgc taaagccgcg gaaattgccg
     3901 ttgcgcatgc tcattatctg agtatcgagt tttatcgaat tgtccgcatc gacccgcacg
     3961 ccgaagaatt cttgagtaat gaacaagttg agcggcagtt gaaaagtgcg atggaacgct
     4021 ggattattaa cgtgctttct gcccaggttg acgatgtcga aaggctaata caaatccagc
     4081 ataccgtcgc ggaagtgcat gcccgcatag gaattccggt agaaattgtc gagatggggt
     4141 ttcgggtgct gaaaaaaatc ctctatccgg taatcttctc ttcggattat tctgccgcag
     4201 aaaaacttca ggtctaccat ttctcgatta acagtattga tatcgctatg gaagtgatga
     4261 cccgcgcgtt tacctttagt gacagtagtg cctcaaagga agatgaaaac tatcgtatct
     4321 tctcgttact agaaaacgcc gaagaagaaa aagaacggca aatagcctca atactttcat
     4381 gggaaataga tattatctat aaaatcctgc tggattctga tttaggcagc agtttgcctt
     4441 taagccaggc tgattttggc ctgtggttta accataaagg tcgacattat tttagtggca
     4501 ttgccgaagt aggccatatc tcccgtctga ttcaggattt cgacggtatt ttcaatcaaa
     4561 ccatgcgtaa caccaggaat ttgaataaca gaagtttgcg ggtgaagttt ctattacaga
     4621 taagaaatac cgtatcgcaa attattacct tgctgcgtga attgtttgaa gaagtatcgc
     4681 gccacgaagt cggtatggat gtactgacga aattacttaa ccgccgtttc ctgccgacta
     4741 tcttcaaacg cgaaattgcc catgccaacc ggaccggtac accgctgtca gtgctgatta
     4801 ttgacgttga taaattcaaa gagatcaacg atacgtgggg ccataacact ggcgatgaaa
     4861 ttctgcgtaa agtctctcag gccttttatg acaacgtccg cagtagtgat tatgttttcc
     4921 gctacggggg cgatgaattt atcattgttt tgactgaagc ttcggaaaac gaaacgttac
     4981 gtaccgcaga acgtattcgc agtcgggtgg agaaaaccaa actgaaagcc gcaaacggcg
     5041 aggatattgc cctctcactt tccatcggtg ccgccatgtt taatggtcat cctgactatg
     5101 agcgcctcat tcaaatagcc gatgaagctc tgtatatcgc caaaagacga ggtagaaacc
     5161 gtgttgaact ctggaaagcc agtctttaga tgcgccagga tgcagaggta atcatgaagc
     5221 taaccgatgc ggataatgcc gccgatggca tttttttccc cgcccttgag caaaatatga
     5281 tgggtgcggt gttaattaac gaaaatgatg aagtgatgtt tttcaacccc gccgcagaga
     5341 agctctgggg gtacaaacgt gaagaagtca ttggcaataa cattgatatg ctgattccgc
     5401 gggatttgcg tcctgcgcat cctgaataca ttcgtcataa ccgtgaaggt ggtaaagcgc
     5461 gcgtcgaggg gatgagtcgg gagctgcagc tggagaaaaa agacggcagt aaaatctgga
     5521 cccgttttgc gctatcgaaa gtgagcgccg aggggaaagt ttattacctg gcgctggtac
     5581 gggatgccag cgtagaaatg gcgcaaaaag aacagacccg acaattgatt attgccgttg
     5641 accatctcga ccgaccggtg attgtcctcg atccagaacg ccatattgtg cagtgcaatc
     5701 gcgcatttac cgaaatgttt ggttactgca ttagcgaagc cagcggtatg cagcccgata
     5761 cactcctgaa cattcctgaa ttccctgccg ataaccgcat tcgtttacaa cagttgctat
     5821 ggaaaaccgc ccgcgatcag gacgaatttc tgctgttgac gcgcaccggt gaaaaaatct
     5881 ggattaaagc ctctatcagc ccggtttatg acgtgctcgc gcatctgcag aacctggtaa
     5941 tgactttctc ggatatcacc gaagaacggc agatccgcca gcttgaaggc aatattctcg
     6001 ccgccatgtg cagcagcccg ccatttcatg aaatggggga aatcatttgt cgtaacatcg
     6061 aatctgttct caacgaatcg catgtttcgc tgttcgcact gcgcaacggg atgccgatac
     6121 actgggcgtc atcttcgcac ggtgcagaag ttcaaaatgc gcaaagctgg tcagcgacca
     6181 ttcgtcagcg tgatggcgcg cctgcgggga tcctgcaaat taaaacctcg tcaggagcag
     6241 aaaccagcgc ctttatcgaa cgcgtggcag atatcagcca gcatatggcc gcgctggcgc
     6301 tggaacagga aaaaagccgt cagcatattg aacaactcat ccaatttgat ccgatgaccg
     6361 gtctgccaaa tcgcaataac ctgcacaatt acctcgatga cctggtcgac aaagccgtct
     6421 ctccggtggt gtatctcatc ggtgttgacc atattcagga tgtgattgat agccttggct
     6481 atgcgtgggc cgatcaggca ttgctggaag tggtcaatcg ctttcgtgaa aaactcaaac
     6541 cggatcagta tctctgtcgt atcgaaggta cgcagtttgt cctcgtgagt ctggaaaacg
     6601 acgtcagtaa cattacccag atcgccgatg agctacggaa tgtggtcagc aagccgataa
     6661 tgattgacga taaacccttc ccgcttacct tgagtattgg catcagctac gacgtgggta
     6721 aaaaccgcga ttacctgctc tccactgctc acaatgcaat ggattatatt cgcaagaatg
     6781 gcggtaacgg ctggcagttc ttcagcccgg cgatgaacga aatggtaaaa gagcgtttgg
     6841 ttttaggcgc agcgctgaaa gaagcgatta gcaataacca actgaaactg gtttaccagc
     6901 cgcaaatctt cgcagaaacg ggtgaactgt acggcatcga agcccttgct cgctggcacg
     6961 atccccagca tggtcatgtg cccccttcac ggtttattcc tctcgcagaa gagattggtg
     7021 aaatcgaaaa tattgggcgc tgggtcatcg cggaagcttg ccgtcagtta gcagaatggc
     7081 gtagccagaa tattcatatc ccggcgttat ccgtgaactt gtcggcgctg cactttcgca
     7141 gtaatcaact gcctaatcag gtgtctgatg caatgcaagc ctggggtatt gacggccacc
     7201 agctgacggt agaaatcacg gaaagcatga tgatggaaca cgataccgaa atctttaagc
     7261 gcattcagat cctgcgtgat atgggcgtgg gtttatcggt agatgatttt ggcacgggct
     7321 tttcaggatt atcccgctta gtcagtcttc cggtaacgga aatcaaaatt gacaaaagtt
     7381 ttgtcgatcg ttgtctgacg gaaaaacgca tccttgcctt acttgaagcc attaccagca
     7441 ttgggcaaag cctcaattta accgtcgtgg cggaaggcgt cgaaaccaaa gagcaatttg
     7501 agatgctacg caagatccac tgtcgcgtta ttcagggata tttcttttcc cgccccctac
     7561 ccgccgaaga aattccaggc tggatgagca gcgtgttacc gctgaaaatc tgacaaattc
     7621 ctctcgcccg cactcgcggg ttttcattta acgtgacact gtcacttaaa tactgtgatt
     7681 tcagctacga ttccgggaga ttccttcctt aacttcccct cttgcaccaa aactgcacta
     7741 cgctgcacaa atgttgatca aaagttaaaa aatataactt taagccagca ttctggcgct
     7801 tatcccggca tggcatgaga tctgcataag cggaaagcgc agcaattttt gtcttataca
     7861 ggaaccgttt atgtcggata ccaccgaact ggttgattta gccgtaatct tccctgatct
     7921 ggagatcgaa ttgaaatacg cctgcgctga taacatcaca ggtaaagcta tttatcagca
     7981 agcgcgttgt ctgttacaca aggatgcgat taccgcgctg gcgaaaagta tcagtatcgc
     8041 ccagctctca ggcttacaac tggtgattta cgatgcgtat cgcccacaac aagcgcaggc
     8101 gatgttgtgg caagcctgcc cagacccgca atatgttgtt gatgtgacgg tcggttctaa
     8161 tcacagccgt ggcacggcga tcgacctgac gcttcgtgat gagcacggga acatcctgga
     8221 tatgggcgca gggttcgatg aaatgcacga gcgctcccat gcctatcacc cttccgtccc
     8281 gcccgccgct cagcgcaatc gcctgttgct gaatgcgata atgactggtg gcggttttgt
     8341 cggtatctcc agcgaatggt ggcacttcga attaccccag gcagcgagtt accctctgct
     8401 cgctgatcaa ttcacctgtt ttatatcccc tggcacgcag cacgtcagtt aatcaggaaa
     8461 ctgtcatgaa gagatcgata tcgtttcgtc ccacattgct cgcgctcgtc cttgccacaa
     8521 ctttcccggt tgcgcacgcc gccgtaccga aagatatgct ggtgattggt aaggccgccg
     8581 atccacaaac cctcgacccg gcggtaacaa tagataataa cgactggaca gtgacctacc
     8641 cgtcttatca gcggctggtt cagtacaaaa cggacggtga taaaggctca accgccgttg
     8701 aaggcgatct ggcaagtagc tggaaagcgt ctgacgatca aaaagagtgg acgttcaccc
     8761 tgaaggacaa tgctaaattt gccgatggca cacctgtcac tgccgaagca gtaaaactct
     8821 cttttgagcg gttactaaaa atcggccagg ggccagcaga agcatttccc aaagatttaa
     8881 agattgatac tcccgacgaa catacggtga agtttaccct tagccagcca ttcgcaccgt
     8941 tcctctatac gctggcgaat gacggtgcat ccattatcaa tccggcggtg ttaaaggaac
     9001 atgcggcgga tgatgctcgc ggcttcctcg cgcaaaatac cgccggttcc ggaccattta
     9061 tgctgaaaag ctggcaaaaa ggtcagcaat tagttctggt gccaaatccg cattactccg
     9121 gcaataaacc gaactttaag cgagtatcgg taaaaattat cggtgaaagt gcctcccgtc
     9181 gcctgcagct ctcccgtggc gacattgaca ttgccgatgc gctgccggtg gatcagctca
     9241 acgccctgaa gcaggaaaat aaagtcaatg tggcagagta tccgtcactg cgcgtcacct
     9301 atctgtatct gaataacagc aaagcgccac ttaatcaggt ggatctgcgt cgggccattt
     9361 cctggtctac cgattatcag ggcatggtta acggcattct gagtggtaac ggaaaacaaa
     9421 tgcgcggccc gattccggaa ggcatgtggg gctacgatgc gacggcaatg caatacaacc
     9481 atgacgaaac gaaagccaaa gccgaatggg ataaagtgac gagcaaaccc accagcctga
     9541 cgtttctcta ctccgataac gatccgaact gggagcctat tgctctggcg acacaatcca
     9601 gtctcaacaa gctgggcatc aatgtgaagc tggaaaagct ggcgaacgcc accatgcgcg
     9661 acagagtggg taaaggtgat tacgacattg cgattggcaa ctggagcccg gattttgccg
     9721 acccgtatat gtttatgaat tactggtttg agtcagacaa aaaaggtctg ccgggtaacc
     9781 gctcgttcta tgaaaacagt gaggtcgata agttactgcg caatgcgctt gcgaccaccg
     9841 accagacgca gcgtacccga gactaccagc aggcacagaa aatcgtcatt gatgacgctg
     9901 cttatgtgta cctgttccag aaaaactacc aactggcgat gaacaaagag gtgaaaggct
     9961 ttgtgttcaa tcccatgctg gaacaggtct tcaatatcaa taccatgagt aaataacgtg
    10021 accttctgga gtattttacg ccaacgctgc tgggggctgg tgctcgtggt ggcgggcgtc
    10081 tgcgtgatta cgtttattat ctcgcatctg atccccggcg atccggcgcg gttactggcg
    10141 ggtgaccgcg ccagcgatgc tatcgtggaa aatattcgcc agcaactggg actggaccag
    10201 ccactgtatg tacagtttta ccgctacgtc agcgacctgt ttcatggtga cctgggaaca
    10261 tccattcgta ctgggtgtcc ggtgctggaa gagttgcgta tatttttccc ggcgacgctg
    10321 gagctggctt tttgtgccct gctgctggca ctcttgattg gcatcccgct gggcatactc
    10381 tctgcagtct ggcgaaatcg ctggctggat catctggtgc gaataatggc gattaccgga
    10441 atctccacac ctgcgttctg gcttgggctg ggcgtcatcg tgctgtttta tgatcatctg
    10501 caaatacttc ccggcggcgg aaggcttgat gactggctgg atccaccaac gcacgttacc
    10561 ggcttttatc tgctcgatgc gctgcttgaa ggcaacggtg aagtcttctt caatgcgttg
    10621 caacatctca tcttaccggc attaacgctg gcgtttgttc acctggggat tgtcgcccgc
    10681 cagatccgtt cagcgatgct ggaacaattg agtgaagact acattcgtac cgcccgggcc
    10741 agcggcttgc ccggctggta tatcgttcta tgttatgcac tacccaatgc gttgatccca
    10801 tcgattaccg tattggggct ggcgctgggc gatttgttgt atggcgcagt gctcaccgaa
    10861 accgtttttg cctggcccgg aatgggggca tgggtagtga catcaataca ggcgctcgac
    10921 ttcccggcag tgatgggatt tgccgtcgtg gtttcgtttg cttatgtgct ggtgaacctg
    10981 gtagtggatt tgctctattt gtggattgat ccgcgtatcg gacgtggagg tggtgaatga
    11041 tgctaagcga ggaaacgtcc gccgtacgcc cagcaaaaca aacgcgattt aacggtgcaa
    11101 tactggtatg gatgctgaaa ggcagtccgc tcaccgtgac cggcgcagtc atcattgtat
    11161 taatgctatt gatgctgatt ttttcaccgt ggcttgcgac gcatgatccc aacgccattg
    11221 atttaaccgc ccgccttttg ccgccttctg cggcgcactg gtttggcacc gatgaagtgg
    11281 gacgcgatct gttcagccgc gtactggtcg gcagtcagca atcaattctc gccggattag
    11341 tggtggtcgc cattgcgggt atgattggtt cgctactcgg atgtctatcc ggtgtgcttg
    11401 gcggacgcgc agacgccatc atcatgcgca tcatggacat tatgctgtcg attccttcgt
    11461 tggtactgac aatggcactg gctgccgctc tcgggccgag tttgtttaac gccatgctgg
    11521 cgattgctat tgtgcgaatt cccttttatg tgcgcctggc gcgggggcaa gcattagttg
    11581 tacgccagta tacctatgtt caggcggcga aaacctttgg cgcgtctcgt tggcatctga
    11641 tcaactggca tattttacgt aactccctgc caccgctgat cgtgcaggca tcgctggata
    11701 tcggtagcgc gattttaatg gccgccacgt tgggatttat tggcctgggt gctcaacaac
    11761 cgagtgctga atggggcgcg atggtagcga atggtcgcaa ctatgtactc gatcagtggt
    11821 ggtattgcgc atttccgggg gcagcgattt tgcttaccgc cgtcgggttt aatctctttg
    11881 gtgatggtat tcgcgatctg cttgacccga aagcaggagg aaagcagtca tgacccaacc
    11941 cgttctggac attcaacagc tgcatttgag tttccccggt tttaaaggtg atgtacacgc
    12001 gctcaacaat gtgtccttgc agattaaccg cggtgaaatt gtcggtctgg tgggagaatc
    12061 cggctcaggt aaatcagtca ccgcaatgct gattatgcgt ctgctaccga cgggcagtta
    12121 ttgcgtacat cgggggcaga tttccctgct gggagaagat gttcttaacg cgcgggaaaa
    12181 acagcttcgt cagtggcgcg gcgcacgagt ggcgatgatc tttcaggaac cgatgaccgc
    12241 cctcaatccg acacgtcgaa taggtcttca gatgatggac gtgatccgcc atcatcaacc
    12301 aataagtcgt cgggaagcca gagctaaagc gattgccctg ctggaagaga tgcaaatccc
    12361 ggatgccgtg gaagttatgt cgcgctatcc gtttgagctt tcaggtggta tgcgccagcg
    12421 ggtaatgatt gcgctggcat tctcctgcga accgcaattg attattgccg acgaaccgac
    12481 tacggcgctg gacgtcacgg tacagttgca ggtactgcgt ttgcttaaac ataaagctcg
    12541 cgccagtgga actgcggtac tgttcatcag ccatgatatg gccgtggtgt cgcaactgtg
    12601 cgatagcgtt tacgtgatgt atgccggaag cgtgattgag agcggcgtga cggcagacgt
    12661 tatccatcat ccccggcatc cgtataccat tggtttgctg caatgcgcac cagaacatgg
    12721 agtaccacgc cagccattac ccgccattcc agggacggta ccaaatctca cccatttgcc
    12781 tgacggctgc gcttttcgcg agcgttgcta tgcgacaggt gcacagtgtg aaaacgtccc
    12841 ggcgctgaca gcgtgtggtg acaacaacca gcgctgcgcc tgttggtatc cgcagcagga
    12901 ggtcataagt gtctgacacg ttattaacgt tacgcgacgt ccatatcaat ttcccggcgc
    12961 gtaaaaactg gcttggtaaa actacggaac atgttcatgc cattaatggt attgatttac
    13021 agatccgccg tggtgaaacc ttagggatcg tcggcgagtc aggctgcggc aaaagcaccc
    13081 tcgcacagct tttaatgggt atgctgcaac cgagccacgg gcagtacatc cgttctggct
    13141 cacaacgcat tatgcagatg gtgtttcagg acccgctctc ttcgcttaat ccgcgcttac
    13201 cggtgtggcg catcatcaca gaaccgctct ggatagctaa gcatagtagt gaacaacagc
    13261 ggcgagcgtt ggcagaggag ctggctatgc aggtgggtat tcgtccggag tatctcgacc
    13321 gcctgcctca tgcgttctcc ggcgggcagc ggcaacgcat cgccattgcc agagcactct
    13381 cttcgcagcc tgacgtgatt gtgcttgatg agccaacctc tgcgctggat atctccgtgc
    13441 aggcgcagat cctcaattta ctggtaacgc tacaggaaaa tcacgggctg acttatgtgc
    13501 tgatttcaca caatgtctcg gtgatacgtc atatgagcga tcgggtagcg gtgatgtatc
    13561 tcgggcagat tgtagaactg ggcgacgcgc aacaggtact gacggctccg tcacatccgt
    13621 acacccgatt attgctggac tccctccccg ccattgataa accgctggag gaagaatggg
    13681 cattacgtaa aacggaactg ccaggaaacc gcacgttgcc gcaaggctgt tttttccgcg
    13741 aacgttgccc ccttgcaacc agcggatgcg aagtccggca atcattagca ataagggagg
    13801 acggacgtga gctccgttgc tggcgggcgc tgtagagatg ctaacaacgc atcaggcatt
    13861 catgcgcctg acgcgtcatc cggcaatgct ttacgatttc aactggtaat ccagcgtaat
    13921 ttccgctttc agtacctgag agaccgggca tcctgctttt gctttctgga tgatgccgtc
    13981 aaaggtagag gcatcaatac ccggcaccgc aacttcactc ttcagtgcga ttttcgtaat
    14041 cgcaaaaccg gcatccactt tatccagcga cacatcggcg gtggtatcaa tcgatgttgg
    14101 cgtgaatcct gcttctccca gcattaatga aagcgccatt gagaaacatg cggcatgcgc
    14161 tgcgccaatc agttcttcag ggttggttcc tttttcgcct tcaaaacgcg tgttaaatcc
    14221 atacggctgt tggttcagca cgccactctc ggtggatact gttcccttcc cgcgtttgat
    14281 atcgccttcc cagtgtgcct gacctttctt atggattgtc attgttgctc tcctgtgggc
    14341 ttaaaaagga aattatagac tacgtgagaa acagcacgag gataagcagg atattccgaa
    14401 taaaatacgg cgaaaatatt acttctgcac ttaaattaaa ttcccaca
//
LOCUS       DAPGOF010000120        14366 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.121, whole genome shotgun sequence.
ACCESSION   DAPGOF010000120 DAPGOF010000000
VERSION     DAPGOF010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.121"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..244)
                     /locus_tag="RHV13_003247"
     CDS             complement(<1..244)
                     /locus_tag="RHV13_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242758.1"
                     /translation="MERVIFSVIFFMMLGVPFYLLYKIARDIFGTAGAGEPAQLPQGK
                     QRRSGGAGSGSIWQGHHLQGSSHLNPLGGDCNGENSI"
     gene            complement(677..1045)
                     /locus_tag="RHV13_003248"
     CDS             complement(677..1045)
                     /locus_tag="RHV13_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242759.1"
                     /translation="MGVDICWRFQREEKPGKWINLSSNYKGDRSYLHFAWLGFDVDRE
                     RASTSAVFIHALRGLPDDIPSEDDDLFGEHSYSWLTSEEILSAIPPDNAGEVIQEFVE
                     EVKRLHVENGSVRFVFGFEG"
     gene            complement(1210..1746)
                     /locus_tag="RHV13_003249"
     CDS             complement(1210..1746)
                     /locus_tag="RHV13_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242760.1"
                     /translation="MKNVNILTTDIAAINALSGVELTDFLTAGYQVNWSGEREVSLVG
                     FPCLASNKQPVEVGPGIFINRVYSKDSQSLCGKLRYDLSNGLASGAFSFFSHSGWYGG
                     FRVVSFRDNGPAALINIGIVHENVLNAFCADFPRANVSVNRFHCGMTLEQIRMFISDT
                     AYDMPLYNNESDFRRLAS"
     gene            complement(1814..2251)
                     /locus_tag="RHV13_003250"
     CDS             complement(1814..2251)
                     /locus_tag="RHV13_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242761.1"
                     /translation="MYRNDRNVDSFNLVNALQPVFRNTGCGIFEYDRKKQNETADSIL
                     RALALNADFARALKQYVRINSVRRVVQFDDGSVRYGIHAEFEGHDKINSFRIFKDEDT
                     DAFDSYFYLVCDNKAELVDFKMNSLDIQLQAVFEKVTGIYLSH"
     gene            complement(2296..2592)
                     /locus_tag="RHV13_003251"
     CDS             complement(2296..2592)
                     /locus_tag="RHV13_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242762.1"
                     /translation="MKKQLAYASNCSDSLYSYIYRTLQKRAGDENESLYQQAISRCRT
                     AKQKKKLAGYYAGPWQLLFNAWCNNRVPNTAVLALLLQQCLSHFQCEEVIAAWQ"
     gene            complement(2713..2919)
                     /locus_tag="RHV13_003252"
     CDS             complement(2713..2919)
                     /locus_tag="RHV13_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000893963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242763.1"
                     /translation="MLESDAAIFLKRAIVARMLQKIKYQDSEVLKDCLGDFFIYEQRE
                     KLRRLGVITNWGINKTADGKNNKL"
     gene            complement(3176..3457)
                     /locus_tag="RHV13_003253"
     CDS             complement(3176..3457)
                     /locus_tag="RHV13_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242764.1"
                     /translation="MKKAVINVDKTNPLPVLTDSVTRVSIQCTAKDENGAGIEVNILD
                     LIQDHDTAEVKVQFGSSPVQALAMLRRVMTALEKQANAELNTNHYSRMQ"
     gene            complement(3523..4707)
                     /locus_tag="RHV13_003254"
     CDS             complement(3523..4707)
                     /locus_tag="RHV13_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDV1242765.1"
                     /translation="MSAIETEVKNNSNEISLRGYLERTGNGARKGNGLIIDPSVVVEQ
                     EGFNTRTAGMGELYYSMPHVVEHLNSLADAYMEDPFSVTPIVVQMVNGVPVLRQGACR
                     IRSIAIANRQLEAEGRERITRIRCEEFRGSASKAELFTLTGNSNLALSVVAEALSIKR
                     LVEDKEEPKTFAELAKKRGKSEQHLRQMVRVLDLPSELQTMLVSDQVSMYVALDEYMY
                     SGEDAVDNINKAISIYGKATKNTLKFVKAAKVEERMADPQTPENIGAEQPADDTTDNT
                     PVPDQLNDESNTETLDTPPVQEPAPAPKDSAPKQNPAGKAPSMSSVLNKKTVTVIADT
                     AIPIFERIAEEAKLKEGESVSTDTYTLVLTNDEMNDIINAHADLKEYLRKHADKMNKG
                     QS"
     gene            complement(5123..5299)
                     /locus_tag="RHV13_003255"
     CDS             complement(5123..5299)
                     /locus_tag="RHV13_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242766.1"
                     /translation="MKDNKTRPVDSYAHADFMESVFSNLRAFCDVEYQRLTAGYPPTV
                     KPEQQSTDGQLSAA"
     gene            5809..6753
                     /locus_tag="RHV13_003256"
     CDS             5809..6753
                     /locus_tag="RHV13_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5417 domain-containing protein"
                     /protein_id="HDV1242767.1"
                     /translation="MKLSRQTTSDTSVDGRSRAYAWGRVHYFIIEHAPMAELVAIDEL
                     LEKAGWSNDGCPNYEKDDEFGNAGYSCGYWIDIDSVGSFKADYKRLKGEISAHIASKA
                     AEVEIRVLDSMSDKECKDVASVASTVRRDLRTQSESLHSLRTIVTVDHYNPYVTTSRP
                     LSISAWTLIHDCLKTGTINDVCSRLSSLILHSEAAIARCKGSSDYSSEHAQLSFFAGN
                     DYVTRRTLVDAAHEEALRMNRRFDERIAMNADSDARRLQCEFNLSNHVVQRRTVESAH
                     IQALNEDVTRSQAEPRCPGKLLLNMTSHEEVRDSLSAC"
     gene            6853..7452
                     /locus_tag="RHV13_003257"
     CDS             6853..7452
                     /locus_tag="RHV13_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242768.1"
                     /translation="MNKEQLIDKLERVVCGQYSYEMQELAYSALCCIKGTPDDCAKFP
                     VTRPLPDTLVDVWDEIGQYLGTGKAIETRSCGVSLLLHGHCYDSNHVLYWRNAGALPA
                     ACDTPLAGGSRKLFTCSACGVDGLDEPPETSCHCCTEGAHWVESRLFTSGQADPEEFR
                     PVAVVKECAGSTPDDRLIWSVIEQLNGELAEGDKLFKRR"
     gene            7512..7862
                     /locus_tag="RHV13_003258"
     CDS             7512..7862
                     /locus_tag="RHV13_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242769.1"
                     /translation="MKKPKAALQNAMKEDVPAVTQIQMRSTVSRKVRYVKQANREGLK
                     LSEWMSRHLDAVCDTADEIHKGKRSTPVAPGKIPPEVYQVIYDQCGGFVECDANAQVI
                     WEACRNAILNGDKG"
     gene            7908..8111
                     /locus_tag="RHV13_003259"
     CDS             7908..8111
                     /locus_tag="RHV13_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242770.1"
                     /translation="MNHESRTVYLNTAIEALLKAEAALNDLALAYELKPDEKASACHP
                     RTGTLSTASQVKKLRRVLEKQKV"
     gene            complement(8530..8688)
                     /locus_tag="RHV13_003260"
     CDS             complement(8530..8688)
                     /locus_tag="RHV13_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HDV1242771.1"
                     /translation="MKMPNQPIVLCILIVCLTLLIFTWLTRNSLCELRMKDGTREVFA
                     ILAYESGK"
     gene            complement(9043..9648)
                     /locus_tag="RHV13_003261"
     CDS             complement(9043..9648)
                     /locus_tag="RHV13_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242772.1"
                     /translation="MTELNKSPSGFSGADVSKEQAAIRALVERVVTNYRSRTAPDRVQ
                     KASVAFNGGLILTLMLALSLLGYLVAELFRGYVADSSPYVPGLSYSQFSMLMIVELCL
                     CFLAWFLVVTGDYPRPWIFQVVPMEQYWFFDEVDDDDLARLSCNPYIKRWLLDEMSGI
                     HRLTYTRLHDRLERICNIPSHLEYSRIQAHKLSLINGTSAS"
     gene            complement(9864..10145)
                     /locus_tag="RHV13_003262"
     CDS             complement(9864..10145)
                     /locus_tag="RHV13_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242773.1"
                     /translation="MKISQLESGMQVWSVTRTKMGNTTITTVIVHPVVIIEIHDNHVI
                     ARWNGNAPRRFGETAIRGWKKEKPLLVREPFGNARLATRAEKAAMQEKE"
     gene            complement(10290..10466)
                     /locus_tag="RHV13_003263"
     CDS             complement(10290..10466)
                     /locus_tag="RHV13_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242774.1"
                     /translation="MKPFGLAGKPRPEPCGCCWYTREDCPYVSAKQIYRRRQRKAERQ
                     RQRSTIVMAVSEFR"
     gene            complement(10520..10831)
                     /locus_tag="RHV13_003264"
     CDS             complement(10520..10831)
                     /locus_tag="RHV13_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242775.1"
                     /translation="MITSLMNFRDLTGEAVIQARQCVINAEIEAAREKVIHARSLFEA
                     GIHNVVNGSSGIKAAAAHFLVIKRLQTDTRYLDAVITDNLCMFSPEGYLYLFMQQRYM
                     R"
     gene            complement(11054..11254)
                     /locus_tag="RHV13_003265"
     CDS             complement(11054..11254)
                     /locus_tag="RHV13_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242776.1"
                     /translation="MKQTKSSMSRIVQLYDGSRYGNCEQADNEGELFTVVLNKPSQID
                     DIRKIVDTTAEVLGKALPVLLL"
     gene            complement(11294..11518)
                     /locus_tag="RHV13_003266"
     CDS             complement(11294..11518)
                     /locus_tag="RHV13_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242777.1"
                     /translation="MHNHEIQTIAIFSAQYKNIEDAENAGALYSVDIEYPMTLNDLSR
                     LCDSIAEAVGVPGGVKYQFVSQPEAHETSF"
     gene            complement(11573..11776)
                     /locus_tag="RHV13_003267"
     CDS             complement(11573..11776)
                     /locus_tag="RHV13_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242778.1"
                     /translation="MKMFFTPYVRIVFYFCALIFIVYSFSDFFQGVPDSTFFSAVIML
                     LVIGTIVARHLDRKKSLKNTSQI"
     gene            complement(12329..12820)
                     /locus_tag="RHV13_003268"
     CDS             complement(12329..12820)
                     /locus_tag="RHV13_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001439478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3085 domain-containing protein"
                     /protein_id="HDV1242779.1"
                     /translation="MTTLVFEMADINKLIEEIRTAKTFSVTADQIYDPACYPGGALLN
                     AEGQTEEEARKAGRVFFPSSSKIASTHLVPKVLLAHSHGVYLITNAELEGSPASRDTV
                     AYAQGMNPKLDEDWDYACDAALGGSDCSYTIPVEWLELAVEQGFQEFRLRMSETKIKL
                     VTK"
     gene            complement(12825..13136)
                     /locus_tag="RHV13_003269"
     CDS             complement(12825..13136)
                     /locus_tag="RHV13_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HDV1242780.1"
                     /translation="MQIEKVMSLLEVLSSWLEDNINMDSEIIFDNDEDNTNSEILYPA
                     VEKANAVLRKMASLSSDSVHAIRQRLQLAVEGKAELSLKDVGELLLATKYLMLSTEEG
                     E"
     gene            complement(13652..13972)
                     /locus_tag="RHV13_003270"
     CDS             complement(13652..13972)
                     /locus_tag="RHV13_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242781.1"
                     /translation="MPNSFDKPQTRPAHNSDAEPFFPKNLGSFIEQVSGRDLAMLTES
                     EKEQLAHLIKIGRRYGVSVAIVPDTDSAELHELLSNAGSIERQFSIYERIQTKIAFTR
                     CQEA"
BASE COUNT         3779 a         3436 c         3345 g         3806 t
ORIGIN      
        1 ctatgctatt ttcaccatta caatcacctc cgagggggtt caaatggctg ctcccctgta
       61 gatggtgtcc ttgccagatc gaacccgaac cagcgcctcc tgagcgtctt tgcttgccct
      121 gaggcaattg tgcaggctca ccagcaccgg cggtcccaaa aatgtcccgg gcaattttat
      181 aaagaaggta aaaaggtacg cccagcatca taaaaaagat tactgaaaat attacacgtt
      241 ccattgatga tctcctgtct tgtgagagac cagttatatc agggtgattc agtatttccg
      301 tttagaaaag agtactaatt tggccctgtt cgatcttaat ttgccatccg ggaaaaagtc
      361 atagggatag gcctgccaga gtcaataact tccagtgatc tggaggagac cccggctctc
      421 tgagccctca tggatggtac ggctttaccc cctgtaaaca gattcacgtt ccgcaggaca
      481 aacatcgact actgacgtct catatttgct gacgctaatc cgacgtctca ttgccgtctg
      541 cttgttttct ttaaagggtg tattagacga atatcaaatc ggaatggtag gggatgtgtg
      601 ggcaccatgc ccggccctct ttgagcatct gagagattgg agacctagcc gggtaagata
      661 gctactttct tacgggttac ccttcaaatc caaagacgaa acggacgctg ccgttctcca
      721 cgtggagacg ctttacttct tcgacaaatt cctggatcac ctctccggcg ttatcgggtg
      781 gtatggctga caaaatttct tccgaagtca gccatgagta tgaatgttca ccgaataaat
      841 catcatcttc ggacggtata tcatcaggta atccgcgtaa tgcgtggata aaaactgctg
      901 atgtgctcgc cctttcacga tctacatcaa atccgagcca ggcaaagtgg agatatgatc
      961 ggtccccttt ataattgctt gataaattaa tccatttacc aggcttttct tccctttgaa
     1021 atcgccagca gatatcaacg cccattaaga aactccatta ctatcgttcg tatgagataa
     1081 ctatcatttc agaatactat gacttcttcc cgtatagaac taatcgctca cgccaactgg
     1141 gcgaagccaa ccggttccgc cacttcgctc attggttttt aggcgtaagc actgaagcca
     1201 gtctccatgc taactggcga gcctgcgaaa gtcagactcg ttgttataaa gcggcatatc
     1261 gtaagcggtg tctgaaatga acatgcggat ctgctcaagc gtcatgccac aatgaaaacg
     1321 gtttacgctc acattggccc gggggaagtc agcacaaaac gcattcaaaa cattttcatg
     1381 gacaatgcca atgttgatca acgcagctgg cccgttgtca cggaaggata cgacacggaa
     1441 tcccccatac cagcctgaat gggagaaaaa tgaaaatgcg ccggatgcca gaccattaga
     1501 caggtcatag cgcaatttcc cgcacagaga ctggctgtct ttgctgtaca cgcgattaat
     1561 gaaaataccg ggaccaactt ctaccggctg cttgttgctt gctaaacacg gaaagcccac
     1621 cagcgaaacc tccctttctc ctgaccagtt tacctgatac ccggcagtga gaaagtccgt
     1681 cagttctacg ccagaaagag cattaatggc cgcgatatcg gtagtcaaaa tatttacgtt
     1741 tttcatattc atctcatctt tattctaaag tgcgtttttt gtctatcagc ccgaggattg
     1801 aaaatttgaa tatttaatgt gacagataaa tacctgtcac tttttcaaaa acagcctgta
     1861 attgaatatc gagagagttc attttaaaat ccaccagctc agctttatta tcgcaaacca
     1921 aataaaaata ggagtcaaat gcgtctgtat cttcatcttt aaaaatacgg aaggaattaa
     1981 ttttatcatg cccttcaaat tcggcatgaa taccatagcg aaccgagccg tcatcgaact
     2041 gaacaacccg tcgtacagaa ttaatccgga cgtactgttt taaggccctc gcgaaatcgg
     2101 catttaacgc caacgctcgt aagattgaat cagcggtttc attctgcttt ttacgatcgt
     2161 attcaaatat cccacagccg gtgttacgaa agaccggctg aagagcatta accaggttga
     2221 aggaatccac gtttctgtcg tttctgtaca tgagatatct cctgcccctc cgctatacga
     2281 aggggcttga tagggttact gccaggctgc gatcacttct tcgcactgga aatgagataa
     2341 gcactgctgg agtagaagag ccagcaccgc tgtgttaggg acgcgattgt tacaccacgc
     2401 gttgaaaagc agctgccatg gaccggcata atacccggcc agtttctttt tttgtttagc
     2461 tgtacggcag cgggaaatgg cctgctgata aaggctttca ttctcatcac cagcccgttt
     2521 ttgcagagtg cgataaatat acgaataaag actgtcagaa cagtttgaag cgtacgccag
     2581 ttgttttttc ataatacctc ctgtttagaa gtattatatg taaacagcct cagcggctaa
     2641 taacccgtag caatataatt tgcatttttt ctatttctga agacgctttt taaattaata
     2701 tttactgagt tatcacaact tattattctt cccgtctgct gttttattaa tcccccagtt
     2761 agtaatcacg ccaagtcggc gcaatttttc acgttgttca taaataaaaa aatcgccaag
     2821 gcagtctttt aaaacttcac tatcctgata ttttattttt tgaagcatcc tcgcaactat
     2881 ggcgcgtttc aggaatatgg ctgcatcaga ctcaagcatt tgtgaggact gtctgacggt
     2941 ctcctgcatg tcgacgatac tgctgagctt tcgtctgacc acgcttagca tagtgtccgg
     3001 gaaccgctta gcggctgaat gtaacccgca acaacgttac gcattgtttt actcactgca
     3061 atgttccatt taagtctgtt tgttccacca gctgctaaca tcagcgttat tcatcctgag
     3121 gtttcgcccg cgatagacag atcagaggca gcatggatga aattgccaga gtagatcact
     3181 gcatacgcga atagtggttt gtattcagtt ctgcgtttgc ctgcttttcc agtgctgtca
     3241 taacccggcg gagcatcgcc agagcctgaa caggcgagct cccaaactgc actttcactt
     3301 cagccgtatc atgatcctga atcaggtcga gtatattcac ttcgatccct gccccgtttt
     3361 catcttttgc cgtgcactga atacttaccc gggtcaccga atccgtcagg actggcagtg
     3421 ggttagtttt gtctacgttg ataacagcct tcttcattgc cctgtttccc cttgtcctga
     3481 aaaagttacc gacccgtccg gatggagggg tcggtcagaa aatcatgact gtcccttgtt
     3541 catcttatca gcatgcttac gcaggtactc tttcaggtca gcatgagcgt tgatgatatc
     3601 gttcatttca tcattggtca gaacaagcgt gtaagtatca gtactgacag attcaccttc
     3661 tttaagcttg gcttcttcag caatacgttc aaaaatcggg atcgcggtgt ctgcaatgac
     3721 ggtcacagtc tttttgttca gaacgctgga catggaaggt gctttacctg ccgggttctg
     3781 tttaggagcg ctgtcctttg gagctggagc cggttcctgt accggtggcg tatccagcgt
     3841 ctccgtattg ctttcatcgt tcagttgatc cggtaccggg gtgttatcgg tagtatcatc
     3901 tgccggttgc tcagcaccga tgttttccgg agtctgcgga tctgccatgc gctcttctac
     3961 cttggcagct ttgacgaatt tcagcgtgtt tttcgtcgcc ttgccataga tggagatcgc
     4021 tttgttgatg ttatctaccg catcctcacc gctgtacata tactcgtcca gtgcaacgta
     4081 catgctcacc tgatcgctca caagcatggt ctgtagttca gaaggcaggt ccagtacgcg
     4141 aaccatctgg cgaagatgct gctctgattt accacgtttt tttgccagtt ccgcaaaggt
     4201 cttaggctct tctttatctt caaccagacg cttaattgaa agcgcttcag ctacgacaga
     4261 cagcgccagg ttggagttgc cggtcagggt gaagagttct gcttttgaag cgctaccgcg
     4321 aaactcttcg cagcgaatac gagtaatgcg ttcacgccct tctgcttcca gctggcggtt
     4381 tgcaatagcg atggaacgga tacggcaagc cccctgacgc agtacaggta cgccattaac
     4441 catctgaaca acgataggcg tcacgctgaa cgggtcttcc atgtaggcat cggccaggct
     4501 gttcaggtgt tccacaacgt gaggcattga atagtagagc tcgcccatac cggccgtgcg
     4561 ggtgttaaag ccttcctgct ctacaacaac cgaagggtca atgataaggc cgttgccttt
     4621 acgggcaccg ttaccagtgc gttccaggta accacgaagg ctgatttcgt tgctgttgtt
     4681 ttttacttca gtttcgattg cggacatttt tgttctctct tttggttggt tgtttaactg
     4741 cgctacaaca caacaatacc aggttcgaca tttttcctaa taggttgcgg agaagaaaaa
     4801 gtaacttttt ttaaaaattt tcctgaggca atactactcg atccgtccgt tttaaatctt
     4861 cacaacagtg ggccgttttt acgtttaagc aaaataactg gttcagaagg tgactcagag
     4921 agaaaagtta cttttccggt tgtctgggcc agtgatggga gagtggcagg acaggaatgg
     4981 ctaaaccggt gcgggtctct tgtaagtcgc aggggattca ggagatatag aggtaaactg
     5041 gtacgttcag tttttggatt gagccgggct tttggcccgg gcttaaggaa gcagaaattt
     5101 gaacgaactg gcaggtaatg ctttatgccg ccgacagctg gccgtcagtg ctttgctgtt
     5161 ctggtttgac agttggggga taaccggcag ttagccgctg gtattccaca tcacagaaag
     5221 ccctgaggtt gctgaagacg gactccatga aatcagcatg agcatacgag tctacaggac
     5281 gtgtcttgtt atctttcatc ttgttctcct caatcattat taaaaccagc ggatttagtt
     5341 tctggcgctc catctgctga ccggctttcc cagagagcag cgatgtgtcg ggttcttttt
     5401 ctcattttat cgttttcatc gggcccatcc gggcgtataa gagaccgatt tggcatgttt
     5461 aggaaacgta ctggcgtaat taaaagctga ggttggattt ggaataaaag gatgcttttc
     5521 ccgtttgtcg ggtctttcgc caccgctgca cgatgattct gtcaggcatt ctgcgccctc
     5581 ccaggctgca catctggaaa caagcgttgg gatgttccct tcccctgctc accgtagaaa
     5641 gatcggcaag tcccgacggt cgttccgtac gagccgggtc caaataatga ataaataaaa
     5701 cgccgcttca attgtattgg caatagaagt caggaggatc atagtgtcat tcatggtgcg
     5761 gctttcgcac caccgccccg gcggtaccgg gcagaactgg agtacctcat gaaactttcc
     5821 cgtcagacga cttctgatac ttctgttgat ggccgttcac gcgcatatgc ctggggccgt
     5881 gtccattatt tcattatcga acatgcacca atggctgagc tggtcgctat tgacgagctg
     5941 ctggaaaaag ctggctggag caatgacggc tgtccgaact atgagaaaga tgacgagttt
     6001 ggtaatgctg gttacagctg tggttactgg atcgatattg acagcgtggg aagcttcaag
     6061 gctgactaca aacgtcttaa gggggagatt agtgcgcata tcgcctcaaa ggcggcagag
     6121 gtggagatcc gcgtactgga ctcgatgtcc gacaaagaat gtaaagatgt tgcctcggtg
     6181 gcatctactg ttcgccgtga cttacgtacg cagtccgaat cgcttcattc actgcgtaca
     6241 atcgtaacgg ttgatcatta caatccctat gtgaccacca gccgcccgct gagcatttcc
     6301 gcctggacac ttatccacga ctgtctgaaa acaggcacca tcaatgacgt ctgttcacgg
     6361 ttgagttcat taatcctgca ctctgaagcc gcgattgccc gttgtaaggg aagctccgac
     6421 tactcttcgg aacacgctca gctatcgttt tttgctggta acgactatgt caccagacgc
     6481 acccttgttg atgcagctca cgaagaggct ctgcgaatga accgccgctt tgatgaacgc
     6541 attgcaatga acgcggattc tgatgccaga aggctccagt gcgaattcaa ccttagcaac
     6601 catgtcgtac agcgtaggac cgttgaatct gctcatatcc aggcactcaa tgaagacgtc
     6661 acccgttcac aggcggaacc acgctgccct ggaaagcttc ttctgaatat gaccagtcat
     6721 gaggaagtgc gggactcact gagcgcctgt tagtccagcg acaccccctg cggctctact
     6781 accgggccgc agtctgctcc tgcccagatg gtgccagtgg tgtaatgaat ttgtttaata
     6841 aggctaagta tgatgaataa agaacaacta atcgacaaac tggaacgcgt ggtatgtggt
     6901 cagtactcct acgaaatgca ggagcttgcg tattcggccc tctgctgcat taaaggcacc
     6961 ccggacgact gcgctaagtt ccctgttacg cgtccgctgc ctgacacctt agtggacgta
     7021 tgggacgaaa tcgggcaata ccttggcaca ggcaaagcca ttgaaactcg cagttgcggc
     7081 gtttcgcttc tgcttcatgg acactgctac gattcaaatc atgtgcttta ctggcgcaat
     7141 gctggcgcac ttccagcagc ttgcgatacg ccactggctg gtgggtctcg caaactgttc
     7201 acctgctcag cctgtggagt ggacggtctg gatgaaccac ctgaaaccag ttgccactgc
     7261 tgcacagagg gggcccactg ggttgagagc aggctcttca cctccgggca ggcagaccct
     7321 gaagaattta gacccgtggc ggtcgtgaaa gagtgtgcag gctcaacgcc ggatgatcgg
     7381 ctcatctgga gtgtgattga acagctcaac ggcgaactgg cggaaggcga taaactattt
     7441 aagcgacgct gacagatcac ggcagcattt ataacagacg aactccagaa taatttaaag
     7501 caaccatgaa catgaaaaag cccaaagctg cattacagaa tgccatgaaa gaagatgtgc
     7561 cagctgtgac acagattcag atgcgatcta cggtttccag aaaggttcgc tatgtaaaac
     7621 aggcaaatcg agagggtctg aaattatctg agtggatgtc gcggcatctc gacgctgtct
     7681 gtgataccgc agatgagatt cataaaggca aacgctcaac tccggttgcg cctgggaaga
     7741 ttcccccaga ggtataccag gtgatatacg atcagtgcgg cgggttcgtg gagtgtgatg
     7801 ctaatgccca ggttatctgg gaagcctgcc gtaatgccat tcttaacgga gacaaagggt
     7861 aaacggcagg tatcgcctgt cattttaccc ttgtgaggac aatactgatg aaccatgaga
     7921 gccgaactgt atacctgaac acggccattg aggccctgct gaaagctgag gcggctctga
     7981 acgatctggc attagcctat gagcttaaac ctgatgaaaa ggcaagcgca tgccatccca
     8041 gaaccggcac actttccact gcgtcccagg taaaaaaact ccgccgtgtc cttgagaagc
     8101 agaaggtatg aagggctgca tcagccaggc ccggatataa atacatgctc tgccaggtga
     8161 gtaccactgc ccggtagccg aatgataagt tttgatttca cggttgcaat ttctccctct
     8221 ggcatcactg ctccacgatg caccccgatc agcttagcgt ctgaaaatga agatgcaccg
     8281 gacctgcatg aagcggaacg gtgcccagct gagtgtttgt gggcacaatg aaccgcaatt
     8341 tcatgatggg aatgaaagat ggctggtggc ccgctgatat gaacgcttac cctgcataac
     8401 gttactaacc acaatctttt cactaaaaga aaaaagggtt cctccgcttt agaggaaccc
     8461 tttcaaaagg tgccgatggc atactaatcc acaccctgaa aagagggggg ttgcccccct
     8521 ctttgtcagc tacttaccgg attcgtaggc caggatagca aatacctccc ttgttccgtc
     8581 tttcatccgc aactcgcaaa gcgagtttcg tgtgagccag gtgaatatca acagcgttaa
     8641 acaaactatt aatatgcaca aaacaatggg ttggttcggc attttcatgg ccttcttctc
     8701 cttgcattta agcaggcaag aggctacgct gtgtgtgact agcatacagg ggcctcgggt
     8761 taattgaaaa attactcggg gctttctgct tttaccacag tcaaacagtg ttaccgctgg
     8821 ctggggcaaa agcctccagc accagcgtca ttatatcact ctgccctgat cagccaaaca
     8881 ccctgcctgc ataaatcatt gatttataat ttatttgaca tgagtcactt ccggttgtcg
     8941 gagacagtct ttttacacta aagaaaaggg taacgggcag ctgcttggtc ggaacataca
     9001 gtgcagttcc tgcccgttta atttccggat gcaatggtag gatcaggagg cactggttcc
     9061 gttgattagg gaaagcttat gtgcctgaat acggctgtat tcgagatggg atggaatatt
     9121 acagatgcgc tccagtcgat catgcagcct cgtgtaggtc agtcgatgaa taccggacat
     9181 ttcatcaagt agccagcgct tgatgtaagg gttacaggaa agccgggcaa gatcgtcgtc
     9241 atccacttcg tcaaagaacc agtactgctc catcgggact acctgaaata tccacggccg
     9301 gggatagtcc cctgtgacaa ccaaaaacca tgcgagaaag catagacaca gctcaacgat
     9361 catcagcatc gaaaattgcg aataacttag accaggcaca tagggtgaac tatccgcaac
     9421 ataccccctg aacaattccg ccacaaggta gcccaatagc gacaaggcca gcatcagggt
     9481 caggattaat cccccattaa acgctaccga agccttttga acccgatcag gtgctgtccg
     9541 gctgcgatag ttggtcacta ctctttctac aagtgccctt atggcagcct gttctttact
     9601 gacatcagct ccggaaaaac cggacgggct tttatttaat tcagtcatac cccactcctg
     9661 ttatgtgctc tactgaacaa acacgtagat catacagagc tcacggacgc actcctgcca
     9721 gataaaggag ggaatttggc tgtaagacgg ttagccaaca gttcaggtta tgcgtgggat
     9781 ccggccagtc agaaaggatt ccgttattaa caggcaaggt taaaggcaga tggacgttga
     9841 aattgtggat gctaaaaggg gtattactct ttttcctgca tagcggcttt ttcagcccgg
     9901 gtggccaggc gagcatttcc gaaaggttca cggacaagca ggggcttctc cttcttccag
     9961 cccctgatag ccgtttctcc aaaacgacgt ggtgcattgc cgttccagcg agcaatcaca
    10021 tgattatcat gaatttctat aatgacaacg gggtggacaa tgaccgttgt aatggtggta
    10081 tttcccattt tggtacgggt tacagaccaa acctgcatcc cggattccag ctggctgatt
    10141 ttcatattat atccttaagc catcacgaac tgcgccacaa aaattaatgt ggcgcaattt
    10201 accataaaca ataaacatca gatacccgtg aagagatttc cctttatttt aaaattcttc
    10261 tggagaaaaa atgatatttc cagaagaagt cagcgaaatt cagaaacggc catcacaatg
    10321 gtgctgcgct gacgctggcg ttctgccttg cgctgcctgc ggcgatagat ctgttttgcg
    10381 ctgacgtaag ggcagtcctc acgggtatac cagcagcagc cgcaaggttc gggccggggt
    10441 tttccggcaa gtccaaatgg tttcatcaag tcaccttttt aatattttta atggcaagat
    10501 acttccctaa cacattgttt tatcgcatat aacgctgttg cataaacaga tacagataac
    10561 cctcaggaga aaacatgcaa aggttatcag tgataaccgc atccagatac cgagtgtcag
    10621 tctgtaaacg ttttatcacc agaaaatgtg ctgccgcagc cttaatgcca gaactaccgt
    10681 ttacaacatt atgtataccc gcttcgaata acgaacgagc atgaattacc ttttcccggg
    10741 ccgcttcgat ctccgcatta ataacgcatt gccgcgcctg gatgactgcc tctccggtta
    10801 aatcgcggaa attcattaga gatgtgatca tgattaactc tccagattat gtggaatcat
    10861 tataacaact ggcatcccga cacaaataat ctgagaaaaa ttctttgaaa tattcctata
    10921 tataaggaat tacagtcgga aaagaaacga tgaaatagcg ccgaccgctt aaaatcgata
    10981 aattaaaatg gacggcactg gaatgaaaaa ctgaaagtag taagaccgta attatccttc
    11041 acggatgtaa gctttataag aggagtactg gcaaagcttt gccaagtact tcggcggttg
    11101 tgtctacgat tttacggatg tcatcgatct gcgaaggctt attcaacacc accgtaaaaa
    11161 gctctccttc gttatcagcc tgctcgcagt ttccgtatcg gctcccgtcg tacagctgca
    11221 caatacgtga catagaagac tttgtttgtt tcatggcttt gcacctgctt ttaggcagtt
    11281 tggttactgg ttatcagaag ctggtttcat gcgcttccgg ctgggacacg aactggtatt
    11341 tgacgccgcc aggcacacct actgcttcgg caatagagtc gcaaagccgc gataaatcat
    11401 ttagtgtcat cggatattca atatctacag aatataaagc acctgcattt tcagcatctt
    11461 cgatgttttt atactgggca ctaaatatag caatggtctg aatttcgtgg ttatgcatga
    11521 accggtcctc attaaattat aaaagccacc cgagagtggc ctgagttgaa acttaaatct
    11581 gtgaagtatt tttgagagat tttttgcgat ccagatgccg ggcaacaatg gttccgataa
    11641 caagtagcat gatgaccgca ctgaagaagg tgctgtcagg gacgccctgg aaaaaatcac
    11701 tgaaactgta aacaataaaa atcaatgcgc aaaaatagaa aacgatacga acataagggg
    11761 taaaaaacat tttcatcggt tgccttccat tcagggagaa ttctcagtaa ttacatcaca
    11821 cctgaagaga aactattttc gtataaatga gcattttcga catatcagag ggggggttta
    11881 ctcataaatg aggtagtaag ggtcacactt gaggtgaaag ttactttcct ggctgcgaac
    11941 gaactatctc aaatgttaga ggtgtgcgtt cgccttgaga gcctcatgcc ctgaggagct
    12001 caacttgagc cgatagtata aacctggctc cagatggctt gctggctggc ataactccac
    12061 gaggccgagc cggaacagga ccggagagct cctgcacgtc acatcatcag gattccccat
    12121 aaacgtacgc cgttctctgg cccgtctgaa attgccagag atatcataga ccgtgccgga
    12181 tgccatccgt cgtaacacgt aaatctgtgc cattgtaagc ttctgggcgg aactgcttaa
    12241 acctgacata ccttcccctc cttatataaa acaatccgcc agcagctcga ctggcggaca
    12301 atgtttgact ggcaacagca aggacatact atttagtgac aagtttgatc ttggtttcac
    12361 tcattcgaag tcgaaactcc tgaaaaccct gctctaccgc taactccagc cactcaacgg
    12421 gaatggtata gctacaatca gacccaccca aagcggcatc acaagcgtaa tcccagtcct
    12481 catccagttt tggattcatc ccctgggcgt aagccacagt atcgcgggat gcgggagagc
    12541 cctcaagctc agcattagtg atcaggtata caccatgact gtgcgcgaga agcacttttg
    12601 gcaccagatg tgtgctggca atttttgagg atgagggaaa gaaaacccta ccagctttac
    12661 gcgcctcttc ttcagtctgt ccctcagcgt taaggagggc tccccccgga tagcatgccg
    12721 ggtcatagat ctgatctgcg gtgaccgaaa acgtttttgc ggtgcgaatt tcttcgatca
    12781 gtttattaat atccgccatt tcaaatacga gagtggtcac aggcttactc tccctcttca
    12841 gtggacaaca tcagatactt tgttgccagc agaagctctc ccacatcctt gagggacaat
    12901 tcagctttgc cttctacggc aagctgcaag cgctgtcgaa ttgcatgaac agaatctgaa
    12961 gataaagatg ccattttgcg caaaacggca ttagcctttt ctacagcagg atacagaatt
    13021 tctgagttgg tattatcttc gtcattatca aagataattt cagaatccat attgatgtta
    13081 tcttcaagcc agcttgaaag cacttcaagt aatgacatca ccttttcaat ttgcatatcg
    13141 cctcctctgt taatgtgaaa caaatataac aaagtggctt acccggctaa taacctgagc
    13201 agaaagtttt cgcactttgt ttgaaaacaa cttttctgct caggttaatg ttaaagtcgc
    13261 tgtgtaaaag ataaagggca ctaaagatga tttaagcatc aaacagacta aatcggattc
    13321 cgtcttttat atcgctttta aaatagaatg aatgaaatat aaatttagcg tttatgattg
    13381 aacgctgtag aggttgattt atccagtgat attcagtttg gggccttaaa aaggttttat
    13441 tttggattcg aattacaaag ggagcaccga catttttctg ggggctcccg cagagaaaaa
    13501 gcggtgaggc tatgaagacg aagaggaggt attcgacgaa atactgtatg gccacaggag
    13561 agcttcagca cccatatcct gccgtctcgc tcgcctgtaa tgatttcaac atgtacaacg
    13621 tcataccacg tagttgtatt tctcactttg tttatgcttc ctgacaccgt gtgaacgcaa
    13681 tcttcgtctg gatgcgttca tagatggaaa actgcctttc aatggaaccg gcattagaca
    13741 gtaattcatg aagctcagca gagtctgtat cgggaacgat agctacagaa actccatatc
    13801 tccgtccaat tttgattagg tgcgcaagct gttctttttc ggattctgtg agcattgcca
    13861 gatctcttcc ggacacctgc tctataaacg aaccaaggtt tttgggaaaa aagggttcgg
    13921 catcagagtt atgtgctggg cgcgtctgcg gcttatcgaa gctattgggc atgaatagaa
    13981 cctatacagt tagagaactg gagaatggat aaagactcta tgaaaatcag ttgtgctaca
    14041 ctcgaatgct aatcaagctt tatgatggct ttagtaggga tggccgtcaa cttaaaccga
    14101 taagaaggga gccatttatc gttaaagtaa ctttcccgga tgatgctctt tttacgaaat
    14161 aagtttctca cactcctgta taagagtcgc cctggaacga gatcgactgg ctttgcttaa
    14221 tgaagagcgt agttgatgtt catcaacccc atcagcgata aactgaccga tttttgtttc
    14281 aagccgctgc attgcctcgt aaagcttggc atccgcatta tttttaagaa acctcagtat
    14341 ataatcagca ataatttgga cgggag
//
LOCUS       DAPGOF010000121        14127 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.122, whole genome shotgun sequence.
ACCESSION   DAPGOF010000121 DAPGOF010000000
VERSION     DAPGOF010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.122"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(73..149)
                     /locus_tag="RHV13_003271"
     tRNA            complement(73..149)
                     /locus_tag="RHV13_003271"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(113..115),aa:Arg,seq:acg)
     gene            complement(153..245)
                     /locus_tag="RHV13_003272"
     tRNA            complement(153..245)
                     /locus_tag="RHV13_003272"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(209..211),aa:Ser,seq:gct)
     gene            complement(561..746)
                     /gene="csrA"
                     /locus_tag="RHV13_003273"
     CDS             complement(561..746)
                     /gene="csrA"
                     /locus_tag="RHV13_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HDV1242782.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(981..3611)
                     /gene="alaS"
                     /locus_tag="RHV13_003274"
     CDS             complement(981..3611)
                     /gene="alaS"
                     /locus_tag="RHV13_003274"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDV1242783.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFVVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAIATVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3739..4239)
                     /gene="recX"
                     /locus_tag="RHV13_003275"
     CDS             complement(3739..4239)
                     /gene="recX"
                     /locus_tag="RHV13_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDV1242784.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4482..5543)
                     /gene="recA"
                     /locus_tag="RHV13_003276"
     CDS             complement(4482..5543)
                     /gene="recA"
                     /locus_tag="RHV13_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDV1242785.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5623..6120)
                     /gene="pncC"
                     /locus_tag="RHV13_003277"
     CDS             complement(5623..6120)
                     /gene="pncC"
                     /locus_tag="RHV13_003277"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HDV1242786.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6265..7350)
                     /gene="mltB"
                     /locus_tag="RHV13_003278"
     CDS             complement(6265..7350)
                     /gene="mltB"
                     /locus_tag="RHV13_003278"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HDV1242787.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7606..8169
                     /gene="srlA"
                     /locus_tag="RHV13_003279"
                     /gene_synonym="gutA"
     CDS             7606..8169
                     /gene="srlA"
                     /locus_tag="RHV13_003279"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDV1242788.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8166..9125
                     /gene="srlE"
                     /locus_tag="RHV13_003280"
                     /gene_synonym="gutE"
     CDS             8166..9125
                     /gene="srlE"
                     /locus_tag="RHV13_003280"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDV1242789.1"
                     /translation="MTHIRIEKGTGGWGGPLELETTPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPVAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            9136..9507
                     /gene="srlB"
                     /locus_tag="RHV13_003281"
                     /gene_synonym="gutB"
     CDS             9136..9507
                     /gene="srlB"
                     /locus_tag="RHV13_003281"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDV1242790.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9511..10290
                     /gene="srlD"
                     /locus_tag="RHV13_003282"
     CDS             9511..10290
                     /gene="srlD"
                     /locus_tag="RHV13_003282"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HDV1242791.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGESMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10396..10755
                     /gene="gutM"
                     /locus_tag="RHV13_003283"
     CDS             10396..10755
                     /gene="gutM"
                     /locus_tag="RHV13_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HDV1242792.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10822..11595
                     /gene="srlR"
                     /locus_tag="RHV13_003284"
                     /gene_synonym="gutR"
     CDS             10822..11595
                     /gene="srlR"
                     /locus_tag="RHV13_003284"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HDV1242793.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDADIDPAFRQALEEKGIDVIITGESNE"
     gene            11588..12553
                     /gene="gutQ"
                     /locus_tag="RHV13_003285"
     CDS             11588..12553
                     /gene="gutQ"
                     /locus_tag="RHV13_003285"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HDV1242794.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTSAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTVGGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLVGAINLQDFYQAGII"
     gene            complement(12550..14064)
                     /gene="norR"
                     /locus_tag="RHV13_003286"
     CDS             complement(12550..14064)
                     /gene="norR"
                     /locus_tag="RHV13_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HDV1242795.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
BASE COUNT         3294 a         3917 c         3530 g         3386 t
ORIGIN      
        1 acggatgcat cgggaaactt actttactgc agattttttg ataccgctac taaagccgta
       61 tcaagtaaga gatggtgcat ccgggaggat tcgaacctcc gaccgctcgg ttcgtagccg
      121 agtactctat ccagctgagc tacggatgca aatggcggtg aggcggggat tcgaaccccg
      181 gatgcagctt ttgaccgcat actcccttag caggggagcg ccttcagcct ctcggccacc
      241 tcaccacacg cctcttacga gtgcttcgaa gaacttgttt atcgctcatc gtcgctgcgt
      301 ggcgcacata ttactttctg agacttataa gtcaaacaat ttttcccaca cttttatcgt
      361 ttgcacactt cacgctcaat tagtctgtaa aaacgtcaaa aagagtgttt tatcaacaga
      421 aaaacggagg tctggcagat agcagtaatg caaaaaaatg gagacttaag ttgaatgaac
      481 gggagtaaag cgaaaagaca atggaatgaa ggagaaattt tgagggtgcg tctcaccgat
      541 aaagatgaga cgcggaaaga ttagtaactg gactgctggg atttttcagc ctggatacgc
      601 tggtagatct cttcacggtg aacagaaact tccttcgggg catttacgcc aatacgtacc
      661 tggttgccct ttacccctaa aactgtcacg gtgacctcat ccccaatcat gagggtctca
      721 ccaactcgac gagtcagaat cagcattctt tgctccttga aagattaaaa gagtcgggtc
      781 tctctgtatc ccggcattat ccatcatata acgccaaaaa gtaagcaatg acaaacacat
      841 tacatctaag cagtcatggc attacattct gttaaaccta agtttagccg atatacacaa
      901 cttcaacctg actttatcgt tgtcgatagc gttaatgcga atgccgtgaa gcgagtccac
      961 ggcattgcct gacgcttata ttattgcaat ttcgcgctga cccagccttt cacactggct
     1021 aacgctgcag gtaaggccgc agcatccgta ccaccggctt gcgccatgtc aggacgtcca
     1081 ccacccttgc cgcccacctg ctgagcgacc ataccaatca gttcccctgc tttcacacga
     1141 tctgtgacgt ccttagatac gcctgcaatc agagaaacct taccttcggc taccgttgcc
     1201 agcacgataa ttgtcgaccc cagctgattt tttaaatcgt caaccatggt acgcaacatt
     1261 ttcggctcaa caccgctaag ctcgctaacc aacagcttaa caccattaac atcaattgcc
     1321 ttactggaaa gatttgcgct ctcctgtgcg gcagcttgtt ctttaagctg ttgtagttct
     1381 ttttctaact gacgcgtacg ttccagtact gagcgcactt tatcagccag attattgcta
     1441 tcgcctttca gcagatgtgc gacttcgctt aagcgatcgc tgtctgcatg aacggtggcg
     1501 atagcacctt ctccggttac cgcttcgata cgacgaacgc ctgcagcagt acccgattca
     1561 gagatgatgc ggaacagacc aatatcacca gtgcggctgg cgtgagtacc gccacacagc
     1621 tcggtggaga aatcgcccat gctcagcacg cgtacgcgct catcatactt ctcgccgaac
     1681 agcgccatcg cacctttcgc tttcgccgct tcgagatcca tgatgttggt ttcgatcggc
     1741 aaattgcgac gaatctgtgc gttcaccagg tcttcgaccg cacgaatctc ttctggtttc
     1801 atcgcttcgt tgtgtgagaa gtcgaagcgc agcaccttgt cgttaaccag tgaacctttc
     1861 tgcgatacat gagtacccag aacctggcgc agcgcagcgt gcatcaggtg cgttgcggag
     1921 tgattcagac gaatacgggc gcgacgagcc tcatcaacat cagcctgcac agcgtcgccc
     1981 actttcagag aacccgcagc aagtttaccg atgtgaccaa tcgcctggcc gtatttctgc
     2041 gtatcttcca ccacgaagga gaagttagcg cctttcagtt cgcctttatc accaacctga
     2101 ccgccggatt ccgcatagaa tggcgtttga tccagcacga ccacagcttc ctggcctgca
     2161 ttgatggcat caaccgcttt accatcaaca aacagcgcgg tcactttgcc gttcagttcc
     2221 agatggtcat agcctttaaa ttcagatgca ctgtcaacac ggatcattgc gttgtaatcg
     2281 gcaccaaagc cgctggcttc gcgcgcccga cgacgctgct cttccattgc tgcttcaaaa
     2341 ccagcttcgt caactttgat gttgcgctca cgacaaacat cagccgtcag gtcaaccggg
     2401 aagccatagg tgtcgtacag acggaaagca gtttcaccat ccagcgtatc accagaaagt
     2461 tttgccagct cttcatccag caacgccaga ccgcgctcca gagtacgagc aaactgctct
     2521 tcttcagtct tcagcacctg ctcaacctgc gcctgctggc gtttcaggtc ttcacccgca
     2581 gagcccataa cgtcgatcag cggaccaacc agtttgtaga agaaggtttc tttcgcgccg
     2641 agcatattac cgtgacgcac tgcgcgacga atgatacgac gcagtacata accacggttt
     2701 tcattggacg gcattacgcc atccgcgatc aggaacgcac aagaacgaat gtggtcagcg
     2761 ataacgcgca gcgatttatt gctcagatcg gttgcaccag tgactttcgc taccgcctgg
     2821 atcagcgtac ggaacaggtc gatgtcatag ttagagttaa cgtgttgcag caccgcagca
     2881 atacgctcca gacccatacc ggtatctaca gacggtttcg gcagcggttc catcgtgcca
     2941 tcggcctggc ggttgaactg catgaagacg atgttccaga tctcaatgta gcggtcgccg
     3001 tcttcttccg gacttcccgg aggaccaccc caaatgtggt cgccgtgatc gtagaagatt
     3061 tcggtgcacg ggccgcacgg accagtgtca cccatctgcc agaagttgtc agatgcgtat
     3121 ggcgcacctt tgttatcgcc gatgcgaata atacgttcgc gcgggatccc tacttctttt
     3181 tcccagattt cgtaggcttc gtcgtcgctt tcatagacgg taacccacag acgctctttc
     3241 ggcagggcaa accatttttc gctggtcagc agttcccatg caaactgaat ggcatcgtgt
     3301 ttgaaatagt cgccgaagct gaagttgccc agcatttcga agaaggtatg gtgacgcgcg
     3361 gtgtaaccga cgttttccag gtcgttgtgt ttaccacccg cacgcacgca gcgttgggaa
     3421 gtggtagcgc gggaataatt acgcttgtcg agcccaagga acacatcctt gaactggttc
     3481 atcccggcgt tggtaaacaa caaagttggg tcgttatggg gtaccaggga gctgctggca
     3541 actacctgat gtcccttact atggaaaaag tcgagaaacg cctgacggat ctcagcggtg
     3601 ctcttgctca taattatcct gaaatcaagc taacgaaata tcgccaccag ctccagcgtg
     3661 tcttaaccgc cgggctggta actgaaaagt gggaataaga taagttttct tgactgggaa
     3721 gtaaaatacc gtatgcgttc agtcggcaaa atttcgccaa atatcctgga tatcttccat
     3781 cagatagcca cgatagagca gaaaacgctg gatcttaact ttttctgaaa agacagttgg
     3841 caaaggttca ccatattttc gcgtcgcctg atcgcgcgcc agtgcgcacc agtcgatgtc
     3901 acattcacgc atcgcttttt ctgtcgcttc gcgggaaata cctttctgat tcagttcctg
     3961 gcgaatacgc gcaggtccat aacctttgcg gctacggctg gcgataaagc gcgcaacaaa
     4021 tcggctgtca tcgagatagc catgttcatg gcaccaggca ataacgcgct cgtaatcttc
     4081 tgccgtagca tcaatctctt ctgggccatt tttgcccata atcggtgccg cgagtttacg
     4141 tcgcagttct tgctcactgt gatcgcgcac cgccagaatg cgtaccgcac gatccaacag
     4201 gcgagcatat gccgggcgac gggacgttga ttctgtcatg gcatatcctt acaacttaaa
     4261 aaagcaaaag ggccgcagat gcgacccagg ttttgctgat aaacagattt aatagtcaat
     4321 gagtgtgcag aatgttcccg caggcccaat tcgttagagg atcgaggtga gagtaatttt
     4381 tcacgccagt gctggcctgt tcccctcacc ctaaccctct ccccaaaggg gagaggggac
     4441 cgtccgtgca cacattatct attatcatca ataacgaacg attaaaaatc ttcgttagtt
     4501 tctgctacgc cttcgctatc atctacagag aaatccggcg ttgagttcgg gttgctcagc
     4561 agcaactcac gtactttctt ctcgatctct ttcgcggttt ccgggttatc tttcagccag
     4621 gcagttgcat tcgctttacc ctgaccgatc ttctcacctt tgtagctgta ccacgcgcct
     4681 gctttctcga tcagcttctc ttttacgccc aggtcaacca gttcgccgta gaagttgata
     4741 ccttcgccgt agaggatctg gaattcagcc tgtttaaacg gcgcagcgat tttgttcttc
     4801 accactttca cgcgggtttc gctacccacc acgttttcgc cctctttcac cgcgccgata
     4861 cgacggatgt cgagacgaac agaggcgtag aatttcagcg cgttaccacc ggtagtggtt
     4921 tccgggttac cgaacatcac accaattttc atacggatct ggttgatgaa gatcagcagc
     4981 gtgttggact gcttcaggtt acccgctagc ttacgcatcg cctggctcat catacgtgcc
     5041 gcaaggccca tgtgagagtc gccgatttcg ccttcgattt ccgctttcgg cgtcagtgcc
     5101 gccacggagt caacgacgat aacgtctact gcgccagaac gcgccagggc gtcacagatt
     5161 tccagtgcct gctcgccggt gtccggctgg gagcacagca ggttgtcgat atcgacgccc
     5221 agtttacgtg cgtagattgg gtccagcgcg tgttcagcat cgataaacgc acaggtttta
     5281 ccttcacgct gcgctgcggc gatcacctgc aacgtcagcg tggttttacc ggaagattcc
     5341 ggtccgtaga tttcgacgat acggcccatc ggcagaccac ctgccccaag cgcgatatcc
     5401 agtgaaagcg aaccggtaga gatggtttcc acatccatgg aacggtcttc acccaggcgc
     5461 atgatggagc ctttaccaaa ttgtttctca atctggccca gtgctgccgc caacgctttc
     5521 tgtttgtttt cgtcgatagc catttttact cctgtcatgc cgggtaatac cggatagtca
     5581 atatgttctg ttgaagcaat tatactgtat gctcatacag tatcaagtgt tttgtagaaa
     5641 ttgttgccac aaggtctgca atgcatacgc agtagcctga cgacgcaccg catcacggtc
     5701 gccgctgaag cattcccgcc gggtaatgcc ttcaccgcgg gcagtggcaa aagcaaacca
     5761 gacggtgccg acaggcttct cttcactgcc gccatccggc ccggcgatac cactaataga
     5821 cacggcataa tcagcacgag ccgctttcag tgcacctatc gccatttcca ccacgacggg
     5881 ttcactcacc gcgccatgct gcgccagcgt ctcttcacgt acgccgatca tctgcgcttt
     5941 ggcttcgtta ctgtaggtga caaatccgcg ttcaaaccag gcggagctac cggcaatatc
     6001 ggtaatcact ttcgctaccc aaccgccggt acaagactca gcagttgtta cggttgcgcc
     6061 acgggctttc agcgcctgcc caacctgttc gcttaactgc atcagttcac tgtcagtcat
     6121 cacaacctct gtcagtcaaa aatttatgcc ggacaagata gcactttcgt caggaagatg
     6181 gtgttggcgt tgcaatttta cgaggaggat tcagaaaaaa gctgattagc cagagggaag
     6241 ctcacgcccc cctctggcaa atagttactg cacgcgcgcc agcgccacgg cttgtcctaa
     6301 ctgccagacc gccattgcgt aatgggtact gtggttataa cgggtaatgg tgtagaagtt
     6361 cggcagaccg taccagtact ggtaacctgt tccaacatcc agacgcagca ggctggcttg
     6421 ttgatggttg cccagcggct gctgtggcgt taaacctgcc gcggcaagct gcgaaatgct
     6481 gtacctggtt ttgaagccat ttggcaagcc tggagcctga ccgtttgcca ttaccgcgac
     6541 ctgatcgcct ttcacccagc cgtgcgcttt gaaataattc gccacgctac cgatcgcatc
     6601 aaccggatcc cacaggttga tatgcccatc gccgctgaaa tccactgcat attgtttgta
     6661 agacgacggc ataaactgtc cgtagcccat cgccccggca aaggaacctt tcagattgag
     6721 cggatcgtcc tgctcgtcgc gcgccatcaa caggaaggtt tccagctcgc cagagaaata
     6781 ctccgcgcgg cgtgggtagt taaatgacag cgttgccagc gcatcgagga tgcgagtttt
     6841 ccccatcacg cgcccccagc gggtttcaac gccgataatc ccgacgataa tttccggcgg
     6901 tacgccatac acctgccacg cgcgattcaa cgcatcttca tactgattcc agaaaaccac
     6961 accgttctgc acgttgtccg gcgtaataaa ttttttgcga taacgcagcc atgcgccgtt
     7021 cggacctgat ggcggtttca ccgatgtcgt tggtgcctgg ttatccatca gccgcagtac
     7081 cgaatccaga cgcttcgcct gggagagaat ttcctgcaac tgctgacgat cgaaaccgtg
     7141 tttgttcacc attttgtcga tgaactgctg ggcattcggg ttattggcga agtcaccgcc
     7201 catctgcatc acattgtgct gcggctcaag caggaagccg ccagacggcg ttccggtggt
     7261 cgtttcagtc tcagtaggtt ttggcttgct gctacaggcg gcaagcaaca caaaaagggg
     7321 aagcaatgtt acataacgac gcttgaacat gaggggtcca tttaacagat tcaaccaggg
     7381 gcaagtatgg taaagcatca cgccccgcac aaggaagcgg tagtcactgc ccgatacgga
     7441 ctttacataa ctcaactcat tctcctcgct atccttttac tcaaactttc aaattaaaat
     7501 atttatcttt cattttgcga tcaaaataac acttttaaat ctttcaatct gattagatta
     7561 ggttgccgtt tggtaataaa acaataaatc ctgaaggaga gaacaatgat agaaaccatt
     7621 actcatggtg cagagtggtt tatcgggctg ttccaaaagg gcggagaggt gtttaccggg
     7681 atggtgaccg gcattcttcc gctgttgatt agcctgctgg ttatcatgaa cgcactgatt
     7741 aattttatcg gtcagcatcg tattgaacgt tttgctcaac gttgcgccgg taaccctgtt
     7801 tcccgttacc tactgttacc gtgcattggc acgtttgtct tttgcaatcc gatgacctta
     7861 agcctgggtc gctttatgcc ggaaaagtac aaacccagct actacgcggc ggcctcttat
     7921 agctgccact caatgaatgg cctcttcccc catatcaacc ctggcgaact gtttgtttat
     7981 cttggcattg ccagcggtct gacaacgctg aacctgccac ttggcccact ggcggtgagc
     8041 tatctgctgg ttggtctggt caccaacttc ttccgcggct gggtgaccga tctgaccacc
     8101 gctatttttg agaaaaagat gggcattcaa cttgaacaaa aagttcatct ggcaggagca
     8161 acatcatgac gcatattcgg atcgaaaaag gaacgggggg ctggggcggc ccgcttgagc
     8221 tggaaaccac gccgggcaaa aaaatcgtct atatcaccgc cggtacccgg cctgcgattg
     8281 tcgacaaact ggcacagctt actggctggc aggctattga cggatttaaa gaaggtgaac
     8341 ccgcggaggc ggaaattggt gtcgcggtaa tcgactgtgg cggcacatta cgctgcggca
     8401 tctatccgaa acggcgtatt cccaccatta atatccactc gacgggcaag tccggtccac
     8461 tggcgcagta cattgtggaa gatatttatg tctctggcgt aaaagaagaa aacatcactg
     8521 tagtgggtga tgcgacacca caaccctctt ccgtgggccg tgactatgac accagcaaga
     8581 aaatcaccga acaaagcgat ggtttactgg cgaaggtggg aatgggtatg ggttctgccg
     8641 ttgccgtgtt gtttcaatct ggtcgtgaca ccatcgacac tgtattaaaa accattcttc
     8701 cgtttatggc attcgtctcg gcgctcattg gcatcattat ggcttctggc cttggtgact
     8761 ggattgccca cggccttgct ccgctggcga gccatccact gggtctggtc atgctggcgc
     8821 tcatctgctc cttcccgctg ctttcacctt tcctcggccc agtcgcagtt atcgcacagg
     8881 ttatcggcgt attgattggc gtgcagattg gtctcggcaa tattccgccg catctggctt
     8941 tacctgcact gtttgccatc aacgcgcagg cggcctgcga cttcattccg gtcggtttgt
     9001 cgctggcgga agcccgtcag gacacggttc gcgtcggtgt cccttctgta ctggtgagcc
     9061 gctttttaac cggcgcgccg actgtactga tcgcctggtt tgtctccggt tttatctatc
     9121 aatagaggct gaaacatgac cgttatttat cagaccacca tcacccgtat cggcgcgagt
     9181 gccactgacg ccctcagcga ccagatgctc atcacctttc gtgaaggcgc gccagcggac
     9241 ctcgaagagt attgcttcat tcattgccac ggcgagttga aaggtgcact ccatcccggt
     9301 ttgcaattta cgctcgggca gcatcgctat ccggtgaccg ctgttggcag cgtggcggaa
     9361 gacaaccttc gcgaactggg tcatgtcacc ctgcgcttcg atggtttaaa cgaagcggaa
     9421 tttccgggca ctgtccatgt ggcaggccct gtacccgacg atatcgcgcc gggatcggtt
     9481 ttgaagtttg aatctgttaa ggagtaaaaa atgaatcagg ttgccgttgt catcggtggt
     9541 gggcaaacct taggcgcgtt cctgtgccac ggtctggctg ccgaggggta tcgcgtcgcg
     9601 gttgtcgata ttcagagcga caaagccgca aatgtggcac aagaaattaa cgccgaatat
     9661 ggtgaaagta tggcgtacgg ttttggtgct gacgccacta gcgagcaaag cgttctggcg
     9721 ctctctcgtg gggtagatga aatctttggt cgcgtggatt tgctggtcta cagcgccgga
     9781 atagcaaaag cagcctttat cagcgacttc cagctcggcg attttgaccg ttcactacaa
     9841 gtgaatctgg tgggttattt cctgtgcgcg cgtgaatttt cgcgtttgat gatccgcgat
     9901 gggattcagg gacgcattat tcagatcaac tcgaaatccg gcaaagtagg cagcaaacac
     9961 aactctggct acagcgcagc gaaatttggt ggcgtcgggc tgactcaatc actggcgctg
    10021 gatctggcag agtacggcat tacggtgcat tcactgatgc tgggtaacct gctgaaatcg
    10081 ccgatgtttc agtcgctatt gccacaatat gccaccaagc tgggtatcaa accggatcaa
    10141 gtcgagcagt attacatcga caaagtaccg ctcaaacgcg gctgcgatta tcaggatgtg
    10201 ctgaatatgc tgctgttcta cgccagtcct aaggcgtcgt actgcaccgg acagtcgatc
    10261 aatgtcaccg gcggtcaggt gatgttctga tcaacagcgg agatccgtta aggatctccg
    10321 tgagactaat aggatgcctg atgcgctacg cgcatcaggc atccaggact tccgccacta
    10381 cattaaggaa aagttatggt atccgcactc atcaccgtcg ccgttatcgc ctggtgtgcg
    10441 caactggctt taggcggctg gcaaatttct cgttttaacc gtgccttcga cacactatgc
    10501 cagcaagggc gggttggcgt gggccgttcc agcgggcgct ttaaaccgcg ggtcgtggtc
    10561 gccatcgccc tggacgatca gcagcgcatc gtcgacacct tgtttatgaa aggactgacc
    10621 gtcttcgccc gaccgcaaaa aattcccgca attaccggta tgcatgcggg tgatttacag
    10681 cccgatgtga tctttcccca tgatccacta tcacagaatg ctctatcatt ggcgcttaaa
    10741 ctgaaacgtg gataatttcg ttgtgaatgt tacttgcttg cgaagttatc attttgaaac
    10801 ctaaatcagg taatcacgcc catgaaacct cgtcagcgtc aagccgccat tctggagtat
    10861 ctgcaaaagc agggaaaatg ctcggttgaa gaattggcgc aatattttga caccacaggc
    10921 acaactattc gcaaagatct ggtcattctg gaacatgccg gaaccgtcat tcgtacttat
    10981 ggcggagtgg tgttgaataa agaggaatcc gatccgccta tcgatcataa aacactcatc
    11041 aacacccaca agaaagagct gattgcagaa gctgccgtta gttttatcca tgatggcgat
    11101 tcgatcattc ttgatgctgg cagtaccgtt ttgcagatgg ttcccctgct ctcgcgcttt
    11161 aataacatca cggtgatgac caacagcctg catatcgtca atgcgctatc cgaactggat
    11221 aacgaacaaa ctatcctgat gccaggcgga acgtttcgta aaaaatcggc ctcatttcac
    11281 gggcagctgg cagagaatgc cttcgagcat ttcaccttcg acaaattatt catgggtact
    11341 gacggcatcg atctcaatgc gggcgtaacc accttcaacg aggtctatac cgtcagtaag
    11401 gcaatgtgca atgccgcgcg cgaagtgatt ttgatggcgg actcatcaaa gtttggccgt
    11461 aaaagcccca acgtagtttg cagtcttgaa agcgtcgata agctgattac cgacgcagat
    11521 atcgatccgg cgtttcgtca ggcgctggaa gagaaaggga tcgatgtgat cataaccgga
    11581 gagagcaatg agtgaagcac tactgaacgc gggacgtcag acgttaatgc tggagttgca
    11641 ggaagcaagc cgcttaccgg aacgtctggg cgatgatttt gttcgcgccg ccaatatcat
    11701 cctgcactgc gaaggcaaag tggtggtttc gggaattggc aaatcgggcc acattggtaa
    11761 gaaaatcgcc gcaacacttg ccagtaccgg cacatcggct ttttttgtcc atccggcaga
    11821 agcgctgcac ggcgatctgg ggatgatcga aagccgcgat gtgatgctgt ttatctctta
    11881 ctccggtggc gcgaaggaac tggatctgat tattccgcgt ctggaagaca aatctatcgc
    11941 gctgctggcg atgaccggca aaccgacgtc accgctgggc ctggcggcga aagcggtgct
    12001 ggatatctcc gtagaacgcg aagcctgccc gatgcacctt gcgccgacct ccagcaccgt
    12061 caataccctg atgatgggcg acgcactagc gatggcagtc atgcaggcgc gcggatttaa
    12121 tgaagaagat tttgcccgct cccacccagc cggggcactg ggcgctcgct tgctgaataa
    12181 agtgcatcat ctgatgcgcc gtgacgatgc catcccacag gtggcgttaa ccgccagcgt
    12241 gatggatgcg atgctggaac tcagccgcac cggtctgggg ctggtggcgg tatgtgacgc
    12301 tcaacaacag gtacaaggcg tctttaccga cggcgattta cgtcgctggc tggttggcgg
    12361 cggcgcactc accacgccag ttaatgaagc gatgacggtc ggcggcacca cgttgcaatc
    12421 gcaaagtcgc gccatcgacg ccaaagagat cctgatgaag cgcaaaatca ctgccgcacc
    12481 ggtggtagat gaaaacggca aactcgtcgg cgcaattaac ctgcaggatt tctatcaggc
    12541 cgggattatt taatccttca atcccagacg tttcgccagc cgatgcaggt tggcgacgtc
    12601 ggtttccagc attcgcgcgc aggctgccca gttgtgatga ttttgtgcca gtgcctgacg
    12661 aatagtttca cgctggaacg cttctgtcgc ttcacgcagg ttttgcttaa caacgggcac
    12721 cgccgccgct tctggcggcg gcaacgtcac ctcaggaaaa gcaaaatgtt gcgcctcaag
    12781 aatcacttca tcgccgttgc gggtggctct cgccagcact accgcccgat gaatagcatg
    12841 ttccagttcg cgcacgttcc ccggaaaacg atagtgttgc agtaaatttc gcgctccggc
    12901 acttaatacc acgcgggaga gccccagccg caaacgacac tgctcgcaga aataccccgc
    12961 cagcagaatg acatcatcgc cccgctcacg cagcggcggc accgaaagtg gaaacacgct
    13021 caggcgatga aacaagtcag cgcgaaatcg ccctgccagc acctcttcgc gtaagtcgcg
    13081 gttagtcgcc gccagcacgc gcacatcgac ccgcaaacta cggtcatcgc caacgcgctg
    13141 aatatcgcca tactgcaaca ccctcagcag cttggcctgc aatgccaacg acaactcgcc
    13201 gatctcatcg agaaacagcg tgccgttatc cgccatttca aacttcccgc tgcggttact
    13261 gatagcgcca gtaaacgctc ctttcacatg cccgaacagt tcactttccg ccacactttc
    13321 cggcagtgcg gcacagttga gatagaccag cggattcacc gcccgtggcg aggcttcatg
    13381 aatcgctttc gccaccagct ccttaccggt tcccgtctca ccgctgatta agacgttgag
    13441 atcggacgcc gccacaatct caatctcttt tttcagttgc gtcatgccag gggacaagcc
    13501 aatcatctgc gtctgtttca ccgcttcaaa cggcgtggca tcgcctggca gcatattctg
    13561 gctttccagt tgttcaatca gcaacgcatt gcttaacgct cccgccgcca gcgcggcaat
    13621 caggcgtaac tcttcgtcgc tgaaaacatc gaactgatcg ggctgcatcc cgtcgagcgt
    13681 caatgcgccg atcaggtttt gcccggcaaa cagcggcaga ccaacgcagg cgtgaacctt
    13741 cagactctcc tgccctggaa tcaaaccgtc atagggatcg ggcaattcgc tgtctgcggg
    13801 aaagcgcacc acatcacccg cgcgggcaat cgcttccagc cgtggatgac cttccagcgc
    13861 aaagcgtcta ccgagtacat ccttcgccag cccgtcgatg gcaagcggaa taaactgccg
    13921 cgagtcgtaa cgtagcaacg cagacgcatc gcactccagc acctgacgca gcgtggtgat
    13981 caggcgctga aaacgatcct gatgaccaat cccacgctgc aattcgatgg cgatattcgc
    14041 cagcacatca acggaaaaac tcatctttgc ctcactgtca atttgactat agatattgtc
    14101 atatcgacca tttgattgat agtcatt
//
LOCUS       DAPGOF010000122        14005 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.123, whole genome shotgun sequence.
ACCESSION   DAPGOF010000122 DAPGOF010000000
VERSION     DAPGOF010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.123"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            29..595
                     /locus_tag="RHV13_003287"
     CDS             29..595
                     /locus_tag="RHV13_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV1242796.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGAIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLLNKDQTNIVLGTAAPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            646..3105
                     /locus_tag="RHV13_003288"
     CDS             646..3105
                     /locus_tag="RHV13_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDV1242797.1"
                     /translation="MDTVNIYRLSFVSCLVMAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNGQKINWQKKGDKTIPCINDSLVDKFGLKPDIRQ
                     SLPQIDRCIDFSSRPEMLFNFDQANQQLNISIPQAWLVWHSENWAPPSTWKEGVAGVL
                     MDYNLFANSYRPQDGSSSTNLNAYGTTGINAGSWRLRSDYQLNKTDSEDSHEQSGGIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKIPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYGGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLT
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            3120..3851
                     /locus_tag="RHV13_003289"
     CDS             3120..3851
                     /locus_tag="RHV13_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDV1242798.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPSFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            3848..4909
                     /locus_tag="RHV13_003290"
     CDS             3848..4909
                     /locus_tag="RHV13_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV1242799.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYTEYADFTSDMLIYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5061..6107
                     /locus_tag="RHV13_003291"
     CDS             5061..6107
                     /locus_tag="RHV13_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDV1242800.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     VTLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAVNWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQHIVWFPPVSINLLLTILLAVVAGTVGCLLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6104..6895)
                     /gene="nei"
                     /locus_tag="RHV13_003292"
     CDS             complement(6104..6895)
                     /gene="nei"
                     /locus_tag="RHV13_003292"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HDV1242801.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEESQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6931..7665)
                     /gene="pxpA"
                     /locus_tag="RHV13_003293"
     CDS             complement(6931..7665)
                     /gene="pxpA"
                     /locus_tag="RHV13_003293"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HDV1242802.1"
                     /translation="MKIDLNADLGEGCTSDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQSGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSAFAEKGIVVAA"
     gene            complement(7655..8587)
                     /gene="pxpC"
                     /locus_tag="RHV13_003294"
     CDS             complement(7655..8587)
                     /gene="pxpC"
                     /locus_tag="RHV13_003294"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HDV1242803.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLRDGDRLPIGKAKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSPQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(8581..9237)
                     /gene="pxpB"
                     /locus_tag="RHV13_003295"
     CDS             complement(8581..9237)
                     /gene="pxpB"
                     /locus_tag="RHV13_003295"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HDV1242804.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9260..10003)
                     /gene="ybgI"
                     /locus_tag="RHV13_003296"
     CDS             complement(9260..10003)
                     /gene="ybgI"
                     /locus_tag="RHV13_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HDV1242805.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10274..11755
                     /gene="dtpD"
                     /locus_tag="RHV13_003297"
     CDS             10274..11755
                     /gene="dtpD"
                     /locus_tag="RHV13_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HDV1242806.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGVVLMIWLYQALKFRNRALALES"
     gene            complement(11905..13323)
                     /gene="phrB"
                     /locus_tag="RHV13_003298"
     CDS             complement(11905..13323)
                     /gene="phrB"
                     /locus_tag="RHV13_003298"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HDV1242807.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAA
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFAASVEIIKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLNEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLKAWQEGITGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRQWLPELRDVPGKVVHEPWKWAEKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(13320..13829)
                     /locus_tag="RHV13_003299"
     CDS             complement(13320..13829)
                     /locus_tag="RHV13_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HDV1242808.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALNDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
BASE COUNT         3349 a         3761 c         3442 g         3453 t
ORIGIN      
        1 tttttctttt ctgttaaagg actccgttat gtttaaagga caaaaaacat tggccgcact
       61 ggccgtatct ctgttgttca ctgcacctgt ttatgcagct gatgaaggat ccggtgcaat
      121 tcattttaaa ggtgaggtta ttgaagcgcc ttgtgaaata catcaggatg atattgataa
      181 agaagttgaa cttggtcagg tgaccaccag ccacattaat cagtcacatc acagcgatgc
      241 cgttgctgtc gacctgcgct tagtcaactg tgatctggaa aactccagca acggttccgg
      301 tggcaagatt tctaaagttg cagtcacctt tgatagctca gcgaaaacca ccggcgcaga
      361 tccgattctc aacaacacca gcacgggtga agccaccggc gtcggcgtgc gtttattgaa
      421 taaagatcaa accaatatcg ttttaggcac tgctgctcca gacatcgacc tggcaccgac
      481 ctccagcgaa caaacgctga atttctttgc ctggatggaa caaattgatc aggctacacc
      541 tgtaacgcca ggtgcagtta cagcgaatgc tacctacgtg ctggattata aataagtatt
      601 actttggcgg cgctatcgcc gcctttcttc aaatatttat caggtatgga caccgtgaat
      661 atttatcgac tctcttttgt atcctgcctg gttatggcga tgccttgcgc attggcggtc
      721 gaattcaacc ttaatgttct cgataaatcg atgcgagacc gcattgatat ttcattatta
      781 aaggaaaaag gagtcattgc tcccggtgaa tattttgtta gcgttgcggt gaataacaac
      841 caaatcagta atgggcaaaa aatcaactgg caaaaaaagg gtgacaaaac cattccatgc
      901 atcaatgatt cactggtcga taaatttggt ttaaaaccag atatccgtca gtccttgcca
      961 cagatagatc ggtgtattga tttcagttcc cgacctgaaa tgctcttcaa tttcgatcaa
     1021 gccaatcagc aactgaatat tagtattccg caagcctggc tggtgtggca ctcagaaaac
     1081 tgggctcccc cctctacatg gaaagaaggt gtcgccggtg tcctgatgga ttacaacctg
     1141 tttgccaaca gctaccgccc acaggacggc agcagcagca ctaacctgaa cgcctacggt
     1201 accaccggaa ttaacgccgg gtcatggcgc ttacgcagtg attaccagct taataagacc
     1261 gatagcgaag atagccatga acagtcaggc ggaatatcgc gcacctatct ttttcgtcca
     1321 ttaccgcaat taggctctaa attaaccctc ggcgaaaccg atttcagttc caatattttc
     1381 gatggttttt cttataccgg cgcggcactg gcgagtgacg atcgaatgtt gccatgggag
     1441 ctacgcggct acgccccaca aattagcggt attgcacaga ccaatgccac ggtgacgatc
     1501 agtcaatcag gccgcgtcat ttaccagaaa aaaattccgc caggcccgtt tattattgat
     1561 gacctcaatc agtctgttca gggcacgctg gatgtcaaag tgacggaaga agatggtcgg
     1621 gtgaacaatt tccaggtttc ggcagcatcg acgcccttcc tgactcgcca gggacaggtt
     1681 cgctataaac tggccgcggg tcagccacgg ccttccatgt cacatcaaac tgaaaatgaa
     1741 acctttttta gcaatgaagt ttcctggggg atgctctcaa acacctcgct gtacggcggc
     1801 ctgctgcttt ctggtgatga ctaccattct gccgcaatgg gtatcgggca aaatatgctg
     1861 tggcttggcg cgctgtcgtt tgatgtcacc tgggccagta gccattttga tactcagcag
     1921 gacgagcggg gcttaagcta ccgatttaac tacagcaaac aagtggatgc caccaacagc
     1981 acgatttcgc tcaccgctta tcgcttctcc gatcgtcatt ttcacagcta cgccaactat
     2041 ctggatcaca aatacaacga cagcgatgcg caggacgaaa aacagacgat cagcttatcc
     2101 gtgggccaac cgattacccc gctaaacctc aatctttacg ccaacctgct gcatcaaacc
     2161 tggtggaatg cagacgcctc cacgactgcc aacatcacag ccggttttaa tgttgatatt
     2221 ggtgactgga gagatatctc gatttcgacg tcattcaata cgacccacta cgaagataaa
     2281 gatcgcgaca accagattta cctgtcgatt tcgctcccct tcggtaacgg tggtcgggtt
     2341 ggttatgaca tgcaaaacag tagccacagc accacacacc gcatgtcgtg gaacgatacg
     2401 ctggatgaac gtaatagctg gggcatgtct gccggactgc aatccgaccg tcctgacaat
     2461 ggagcccagg tgagcggtaa ctatcagcac ctgagttcag caggtgagtg ggatatttct
     2521 ggtacctatg ccgccaatga ttacagttcc gtcagcagca gctggagcgg ttctttcacc
     2581 gcaacccaat atggtgcagc gtttcatcgc cgcagctcca ccaatgaacc acgcctgatg
     2641 gtcagcaccg atggcgtggc agatattccg gttcagggca atctcgacta caccaaccat
     2701 tttggcattg cggtggtgcc gttgatttcc agttaccagc cttccaccgt ggcggtgaac
     2761 atgaatgact tacccgacgg cgttacagtt gcagaaaacg ttatcaaaga aacgtggatt
     2821 gaaggcgcga taggttacaa atcactggct tcccgttccg gtaaagacgt taacgtcatc
     2881 attcgcaacg ccagcggtca gtttcctccc ctcggagcgg atatccgcca ggatgacagc
     2941 ggcattagcg tggggatggt tggcgaggaa ggacatgcct ggttaagcgg agtcgctgaa
     3001 aatcaaaagt ttaccgtggt ctggggtgat agccagcatt gctcgctcca tcttcctgaa
     3061 catatggaag acaccgcaaa tcgcctgatt ttaccttgtc attaattaag gaaatgaaaa
     3121 tgacatttat gaaaggacta ccccttttgt tgttggttgc cagtctgtgc agccacgctg
     3181 cactacaacc cgatcgcact cgtattgtgt ttaacgctaa tgataaagct accagtctgc
     3241 gggtggataa tcgtagcgat aaactcccct acctcgctta ttcctggctc gaaaatgaaa
     3301 aaggggagaa aagcgatgat cttctggtcg ccctgccgcc gatacaacgt ctggaaccga
     3361 aagcaacaac gcaggtacgg atagtaaagc aagcctcgac cacaaaatta ccgggcgatc
     3421 gcgaaacgct atttttctac aatatgcgtg aaattcctcc cgccccggaa aaaaacagcg
     3481 accatgccgt tctgcaagtc gccattcaga gtcgtatcaa ggtgttctgg cgaccggctg
     3541 cattacgcaa gaaagcagga gaaaaggtcg aactgcagtt acaggtcagc cagcagggca
     3601 accaactgac actgaaaaat cctaccgcgt attatctgac cattgcctac ctgggacgaa
     3661 atgaaaaagg cgttctcccc agcttcaaaa ccgtgatggt tgcgcctttt agtacggtca
     3721 acacaaacac cggaaattat agcggcagcc agttttatct cggttatatg gatgattacg
     3781 gcgcgttacg catgacgacg ctcaactgca gcggacaatg ccatttacaa gccgtggagg
     3841 cgaagaaatg agtgctggca agggagtgtt gctcgtcatc tgtctgttat ttctgccgtt
     3901 gaagtctgcg cttgccctga actgctactt tggttcatcc ggtggctcgg tagaaaaatc
     3961 agaagcgatt caaccttttg ctgtaccagg caatgccaaa ctcggcgata agatttggga
     4021 atctgacgat attaaaattc ccgtctattg tgacaacaat accaacggca attttgaaag
     4081 tgagcatgtt tatgcctggg taaaccccta cccgggggtg caggatcgct attatcaact
     4141 cggcgtaact tacaatggcg ttgattatga cgccaaccag ggaaaaagcc gcatcgacac
     4201 taaccagtgt atcgacagta agaacattga catttatacc cctgagcaga ttatcgcgat
     4261 gggatggcag aacaaaattt gctcgggtga tcccgctaat attcacatgt cgcgtacctt
     4321 tctcgctcgc atgcggttat acgtcaaaat acgagaaatg ccgccgcatg attatcaaag
     4381 tacgcttagc gactatatcg tcgtgcaatt tgacggtgcc ggtagcgtta acgaagatcc
     4441 tactgcccaa aacctgaaat atcatattac cggtctggaa aacattcgcg tgctggattg
     4501 cagcgtcaat ttttccattt caccggaaac acaggtgatt gattttggta aatttaattt
     4561 gctggatatt cgtcgccaca caatgtcgaa aacattcagt attaagacga caaaatcaca
     4621 aaatgaccaa tgcaccgacg gatttaaagt cagttcctcc ttttataccg aagaaacgtt
     4681 ggttgaagaa gataaagcat tgttgattgg taatggtctg aagctacgtt tgctggatga
     4741 aaacgcctcg ccttatacat tcaataaata tactgaatac gccgatttca ccagcgatat
     4801 gctgatctac gaaaaaacct atacggctga actttcgtcc attgcaggca cccccatcga
     4861 ggctggcccc ttcgatacag tggtgctttt taagattaac tataactgac ttgccatgat
     4921 gccctttgca gccggaaagg gcatctcttt cagaaaattg ctgattttcc ccttttcctt
     4981 gattgctgtt attttttcct ggcccggctt tctttatgct gtatcgatac accaaagaga
     5041 aaataatgag ggagcaaagg atgccagttt tgcagtgggg aatgttatgt gtgctatcac
     5101 ttctcctttc tattggtttc ctcgcggtac atctcccggc ggcattatta cttgggccga
     5161 tgatcgccgg gatcatcttt agtatgcgcg gggttaccct gcaactcccc cgctccgctt
     5221 ttctcgccgc gcaggccatt cttggctgca tgattgcgca aaacttgacc ggttcgatcc
     5281 tgaccacgct ggcggttaac tggccgattg tgttagctat tttactggtg acgttgcttt
     5341 ccagcgctat cgtgggatgg ctactggtgc gctatagctc actgcccgga aatactggtg
     5401 cctggggctc ctcgcctggc ggggcggcgg caatggtcgc gatggcgcag gattacggcg
     5461 cagatatccg cctggtggcg tttatgcagt atctgcgagt gctgtttgtc gcgggggccg
     5521 cagttctggt cacgcgaatg atgctgggcg ataacgctga agcggttaac cagcatatcg
     5581 tctggttccc gccagtgagc attaatctcc tgcttactat tttgctcgcg gtcgttgccg
     5641 gtacagtggg atgcctgctg cgtcttcctt ccggcacgat gctcatcccg atgcttgcgg
     5701 gcgcagtgct ccagtctggt cagctcatca ccatcgaact accggaatgg ctgctggcga
     5761 tggcgtatat ggcaattggc tggcggattg gtcttggttt cgataaacaa atattactgc
     5821 gggcattgcg cccactgcca caaatcctgc tgtcgatttt tgctctgctg gctatttgtg
     5881 cgggtatggc gtggggactg acccggttta tgcacattga ttttatgact gcctacctcg
     5941 ccaccagccc cggcgggctt gataccgtgg cggtcatcgc cgcagggagc aatgccgata
     6001 tggcgctcat tatggcgatg caaaccctgc gcctgttcag tattttgctg actgggcctg
     6061 ccattgcacg gtttatttcg acctatgcgc cgaagcggtc ggcctagtgc tggcagcctg
     6121 gacaccagta aaacgggcgc gaggacaacg tggttttctc aatgatgccg ccacagcgtt
     6181 cgcacagttc gccatcacga tgaaaaacct taaagcgaaa cagcgcccca tgatgcttat
     6241 tctcatccac ctgcccccgc gtagcgtaag aaaatcgagg aatctccagt aacgcgtgtg
     6301 cgagtgcatc cagttgcgcc gcattgagat ctttcgcttt atgatttcca gtcaacccaa
     6361 cctgccagag gatctccacc cgcaaataat tgccaagtcc tgccagaaac gcctgatcga
     6421 gcagtaatcc agcaaactga cggttacgaa agcgcggcga caataaccgt tctttcacca
     6481 cctccggcgt cagattcgga tccagcacat cgggaccaac gcgttgtaaa aacggatgcg
     6541 tggtcagttg ttccggcctc aacatctcaa tatccgaggc gctataaagc agaatggttt
     6601 tgtcagccgt ttgcagtttt acccgcaata ctcgcgtggt ctgcgactct tcgccggtat
     6661 caaccacgcg ccagacgcca taaagctgat tatggctgta gagcgttaag tcgttggaaa
     6721 aatgagttaa caacgcctta ccacgcgttt ccacatgggt aacgtgttga ccgataagtt
     6781 gtgattgata agtttttaac tgcgggaagg caaaccagac atcagttagt ggtttgcctt
     6841 tgatcgccgc ctccaggtta tccgctgcac ggcggatctc cgggccttca ggcatggtga
     6901 tatccttttt tcattcttta ttaatcgggg ttatgctgcg acaacaatcc ccttttcggc
     6961 aaatgcagag cgtagtcggc gggcgaacgc cagtgcgtgc tcgccgtcgc catgcaggca
     7021 gaccgtttgc gccgtgaccg ttgcccattc gccggtgata cttttgactc tgccgtgttg
     7081 caccatctcc agcgtttgcg ccagcgcctg ttcttcgttt tcaatcaacg cgcctgactg
     7141 gcttcgcggc accagcgagc cgtcagcctg ataaccgcga tcggcaaaca cttcctcgcg
     7201 cgttgtcaga ccatattgct tgcctgcacg aatcagctcg cttcctgcca gcccgacgag
     7261 aatcaatgcc ggatcgcaag cgtataccgc tctggcgatg gcgtctgcca gttgtgcttc
     7321 tttcgccgcc tggttgtaca acatgccgtg cggtttgaca tgacgcatta cgccgccttg
     7381 cgcacgggca atcgttgcca gcgcgccaat ttgatacagc gtctgggcgt aaacggtttc
     7441 tggcggcagc tgcatagcgc tgcgaccaaa attttccctg tcgggaaaac tcggatgagc
     7501 gccaatcgcg acaccatttt ttattgcttc acgcacgcaa gcctgcatgg tttgcgcatc
     7561 gcctgcatga aatccacagg caatattggc agaggaaacc agcgttaata gctctgcgtc
     7621 actggtgcag ccttcgccca gatcggcgtt caggtcaatt ttcattgtgc agccgccacg
     7681 ctaattgttc gaaataacgt tgctgatctt gccgcgcttt cagtgcctct tccagtgaac
     7741 actggacaaa atgaatcggc tgaccgagcg gaatttgcgc cagatggtac atatcagcct
     7801 caatgataca ggcaatacgc gggtaaccac cggtggtctg tgcgtcgttc atcaacacaa
     7861 tgggctgccc gttatgtggc acctgcacca cgcccggtaa caaaccgtga gataacagtt
     7921 cgcgatcggt ggtgcgtttt aaaatttgcc cctgtaagcg atagcccatg cggttacttt
     7981 gcgggctaag ctgccagggc gaacgccaga atgcatcctg cgaggcgcga tcgaactcat
     8041 gatattccgg ccccggcaag gcgcgaatgc ggttgcccca cagcagctgt ttaacgcctt
     8101 gcgcttccat aaaatcacgc ttcgctttgc caatcgggag ccggtcacca tccctcagta
     8161 aacggccttc cagcccgcca atccccactt tgagatcggt gctgcatgat cccattaccg
     8221 gcggaacatc aataccaccc gcgaccgcca gataactgcg catcccgtgc tgcgggcgtt
     8281 taagcgttaa acgctggcct gctttcatcg gcaatcgcca gccggtccag acggcattat
     8341 catccagccg cgcttcgcaa ccggcacccg tcagagcaaa ccacccatca gtttcgaact
     8401 caaccgttaa ctgaccgagc gtgatctcca gcgcgggggc attggcgtca ttacccacca
     8461 gcaggttagc aatgcgtaac gcaggcatat ccagtgcgcc gcagtggctg atacccgact
     8521 ggcgaaaacc gtgacgaccg ccatcttgca cagtggtata catgcccgca cgaataatct
     8581 tcagcatact ccctcctttt gcggaacaaa gcgcacgctg tctcccggac gtaataagat
     8641 gggttcgtca cgcgccggat caaacaggct gagtgaggta tgaccaatca actgccagcc
     8701 acccggcgtt gccagcggat aaacaccagt ctgaggcccg ccgatcccga cagaacctgc
     8761 cggaacgagc aagcgcggtt cagcgcgccg tggcgtgtgt agttgttccg gcaaactccc
     8821 gagatacggg aagcccggtt gaaaacctaa aaaccagacc acgtattcca cggaggagtg
     8881 caattcaaca acctgttttt cgctcaaccc gcaatgcgcc gcgaccaccg ccaaatccgg
     8941 tccgcctgca ccaccgtaaa ccaccggaat ttcaataaag cgagactcag gttccagggc
     9001 ctcgctctcc tcccaccagc gttgcaaacg ctctatggca tccagcgcca gcgactcagg
     9061 attacgcaaa atcaccgtga tattgttcat gccgggaatg gcttcaacca cattcggcat
     9121 atccaccaga cgctgcgcca gtcgccagat ccgtttctgg ctagccagcg tcaccggcgg
     9181 ttccagttcc agcactaccg ccgtttcacc tatcagataa caacgcgctc gttgcacttt
     9241 cgatccctct gattattcgt tatgcaggat taggaatatc aataaaagtc acatcaagat
     9301 cggtattttc attcagccac tcgctcaatg cgcgaatacc accacgttca gtggcatggt
     9361 gacctgcagc ataaaaatgc aatccctgct cgcgggctga atgaatggtc tgttcagaaa
     9421 cttcgccagt aataaaagca tccacgccaa aacgcgcggc gctatcgata aaactttgcc
     9481 cgccgcccgt gcaccaggcg acgcgctgga ccacctcagg tccggtatcg ccacaccata
     9541 acggcttacg tcccagacgc gcttcaatcc aggaagccag ctccagtccc ggcacaggca
     9601 tggtcagctc tccccacggc accaacggct caatttcgcc catgaccgtg atccccagta
     9661 acgccgccag ctgtgcgtta ttgcccagct caggatgtgc gtcaagcggc aaatgccagc
     9721 catacaggtt gatatcattt gccagcaacg tttttaaacg gttacgcttc atgccgcgaa
     9781 tgaccggaga ttcacctttc cagaagtagc catgatgcac aatgaccgca tccgcgccca
     9841 gacgcactgc ctcatcgagc aaagcctggc tggcagtgac accggtaaca attttttgca
     9901 ccgtctcttt gccttccacc tgcaaaccat tcggcgcgta atcactaatc gccgcgctgt
     9961 tcagtttttc gttaatcagt tgttccagtt cggtgttttt catcttcgcg ccttccgtat
    10021 tctgatagct acaccgttaa catagcgtta cctcgcctgt tcgtcgatag ccaatctttt
    10081 gacatattaa gaaacaaacc cgacgccaaa agacgcaaat tgtttatatc atcacgcttc
    10141 agctaatgta tatttatgca atcagacagc atattgcata aataatcaga tctgtatcaa
    10201 agttttaact ttaaggaaac acaagcacgc cgcgcaacta ttacgtgacg ttgccgtcaa
    10261 ttgtggaatt attatgaata aacacgcatc acaaccgcgc gctatttact atgtcgttgc
    10321 gctgcaaatc tgggaatatt ttagctttta cggcatgcgt gccctgctga ttctctatct
    10381 caccaatcaa ctaaaataca acgatactca cgcctacgag ttatttagcg cctactgttc
    10441 gctggtgtat gtcacgccaa tcctcggtgg ctttttggcg gacaaagttc tcggcaatcg
    10501 catggcagtg atgctggggg cgttgttgat ggcgatcggt catgtggtgt tgggtgcaag
    10561 tgagatccat ccgtcattcc tctatctgtc cctggcgatt atcgtttgtg gctatggtct
    10621 gtttaaatcc aacgtcagtt gcctgctcgg cgagctgtat gagccaaccg atccgcgtcg
    10681 tgatggcggt ttctcgctga tgtatgcggc gggtaacgtg gggtctatta ttgcacctat
    10741 cgcctgtggt tacgcccagg aagagtacag ttgggcgatg ggctttggcc tggcggcggt
    10801 gggtatgatc gcgggtctgg ttattttctt atgtggcaat cgtcatttca cacatacccg
    10861 cggcgttaac aaaaaagtac tgcgtgcgac aaactttctg ctgccgaact ggggatggct
    10921 gctggttctg ctggtggcaa cgcctgcgct gattaccgtt ctgttctgga aagaatggtc
    10981 ggtatacgcc ttgattgtcg cgactatcat tggcctgggt gtactggcaa aaatttatcg
    11041 caaagcagaa aaccagaaac agcggaagga gctggggctg attgtgacgc tcaccttctt
    11101 cagtatgttg ttctgggcct tcgcacaaca gggcggtagc tcgattagcc tttatatcga
    11161 ccgcttcgtt aatcgcgata tgtttggtta taccgttccg accgcgatgt tccagtcgat
    11221 taatgccttc gcagttatgc tgtgcggtgt gttcctggcg tgggtggtaa aagagagtgt
    11281 cgcgggtaat cgtaccgtgc gcatctgggg aaaatttgct cttggccttg gcctgatgag
    11341 cgccggattc tgcattctga ccttaagcgc ccgctggtcc gcaatgtatg gtcactcttc
    11401 tctgccactg atggtattag gcctggcggt gatgggcttt gcggaactgt ttatcgaccc
    11461 ggttgccatg tcgcaaatta cgcgcattga aatccccggt gtgaccggcg tattaaccgg
    11521 catttatatg ctgctttccg gcgcgattgc gaactatctg gcgggcgtaa ttgccgatca
    11581 gacatcgcag gcttcgtttg atgcttccgg ggcgatcaac tactccatca atgcatatat
    11641 tgaagtattt gatcaaatta cctggggcgc actggcgtgt gtaggagtgg tactgatgat
    11701 ttggctgtat caggcgctga aattcagaaa ccgcgcgctg gcgctggagt cttaataggc
    11761 cagcaccgat gcctgatgcg acgcttgacg cgtcttatca ggcctacgta tttcctgcaa
    11821 tttattgaat ttgcacaaat ttgtaggctg gataaggcgt tcacgccgca tccggcatct
    11881 gggctcgatt gcctgacgcg tctgttattt ccccttccgc gccgcctcat acgctgccaa
    11941 cgtttgtact ctcgcctctt tgtgctcgac tatcggttgc ggataatcca gcttcacacc
    12001 tgctttctca gcccacttcc acggctcatg caccactttc cctggcacat cgcgcagttc
    12061 cggtagccac tggcggataa actcgccttc gcggtcaaat ttctcgccct gggttgtcgg
    12121 gttgaaaata cgaaaatacg gcgctgcatc agtacccgta gaagccgccc actgccagcc
    12181 accgttattg gctgccaaat caccatcaat cagctgcgac atgaaatatc gctcgccttc
    12241 gcgccagtcg atcaataaat ctttcaccag aaaactggct gtaatcatcc gtagccggtt
    12301 atgcatccag ccagtgctat taagctgacg catggcagca tcgacaatcg ggtatcccgt
    12361 tatgccttcc tgccaggcct ttaaatgggc gggattgctt tgccactgta cacgatccgt
    12421 ccaggcaata aatggacgat gtttacacaa cgaggggtaa tacgtcatca gatgacggta
    12481 gaactcgcgc cagatcagct cattaagcca gacactaccg gccccaccgt ccagcgcctg
    12541 cggctgttcc gccaacaagc gatgcaaaca ctggcgaggc gataacccgc ccgttgccag
    12601 gctggcggac aaacgactgg tgccttccac tgccggaaaa tctcgttgtt gctcatattc
    12661 tccggcaccg ttctggcaaa actggcgtaa ttgcgcaatc gccgcttttt cttccaccgg
    12721 gaaatgcgca gtatcgaaag actgacgagg ataattcagc gtaatggatg gcgcgggctt
    12781 tatcgatccg ctactacgaa cttttggtgc agcgacgcac tccggcatcc cttcccgcag
    12841 ccgtttcagc caggcattct taaaaggcgt aaagactttg tacatctcat gattgccggt
    12901 catcaccgcg ccaggcggca ggatcacgct gtcatcaaat ccttcacaca ccacgttacg
    12961 cagcgctctt tcaacttcca catcccgcgc ccgctcattc acctcatact gatagttata
    13021 aaaaagatga gtaacgctgt tttccgcgca cacctgttta attatttcga cgctggcggc
    13081 aaagtcatcc acttcacgga acaataaagg aatacctttt tccgccagcg cgatttgtag
    13141 cccgttcagt tgagcattga taagttcagc ctgacgcggc gacatattat gcgccgccca
    13201 ctggcgtggt gtagcgatat acaacgccag cacgcgtgca gacgaattgc gacaggcggc
    13261 agccagcgcg agattatcgt gcagacgtaa atcctggcga aaccagacca gatgagtagt
    13321 cataaaactc ctgaatggcg caagttaatc acacgcggcc aaagttcgaa ataacgctgc
    13381 cctgaaagcc aggcgtcagg ataaagcgcc atatagtgtt tgatacgcat cagcggcgca
    13441 agaagcggtt gctcgccacg gcgataactg tcgataagcg ctgccagctg ctggcgttct
    13501 gtggaatcaa tatgcgggcg aaaataaccc tgaacgtgca tcaaaacgtt ggtgtgatcg
    13561 cgcgggttgg cgggatgaga aagcaaaaca attacgcgtt ggcggtactg gttgtaaaaa
    13621 tcatccagat tttgccactg gtgaatatca gccacgaacg ggccaagttt gcggtattcc
    13681 ggctgggaat gagccagaaa gacgagttta tagcggctgt gataatcgtt gagcgccccg
    13741 cgcgttaagc catgttcttt cagtcgatgt aactcatgaa gggcgaagat acggattagg
    13801 gtgctgtcat caaatcgttg ctgattcatt actgctcctg aacggtttac aggagtaagt
    13861 gtagacgatg gtaaatgaat tttgtggggt tatgctttct tccctaccaa gctgttatat
    13921 ccagttcaaa aacaatcatt ttacatcatc atcaaagcag cgaatacttg accgattggt
    13981 agtaccgctg catatttcac catca
//
LOCUS       DAPGOF010000123        13894 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.124, whole genome shotgun sequence.
ACCESSION   DAPGOF010000123 DAPGOF010000000
VERSION     DAPGOF010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.124"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(98..853)
                     /gene="mlaA"
                     /locus_tag="RHV13_003300"
     CDS             complement(98..853)
                     /gene="mlaA"
                     /locus_tag="RHV13_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HDV1242809.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            1147..2079
                     /locus_tag="RHV13_003301"
     CDS             1147..2079
                     /locus_tag="RHV13_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDV1242810.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            2155..2229
                     /locus_tag="RHV13_003302"
     tRNA            2155..2229
                     /locus_tag="RHV13_003302"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2188..2190,aa:Arg,seq:cct)
     gene            2391..2672
                     /locus_tag="RHV13_003303"
                     /pseudo
     CDS             2391..2672
                     /locus_tag="RHV13_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311258.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
     gene            complement(2743..3168)
                     /gene="tnpA"
                     /locus_tag="RHV13_003304"
     CDS             complement(2743..3168)
                     /gene="tnpA"
                     /locus_tag="RHV13_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEc46 family
                     transposase"
                     /protein_id="HDV1242811.1"
                     /translation="MSNHDDLLAGFLRKRHSVSKLVVHLIFTTKYRRKLFDSQMIAQL
                     REAFGSAAAKLECEIIEMDGEPDHVHLLVAYPPKLAVSVMVNNLKSVSSRLLRQQNAH
                     LRMQSKTGLLWSRSYFVCSTGGATIETLRAYVQSQSTPD"
     gene            3225..4394
                     /locus_tag="RHV13_003305"
     CDS             3225..4394
                     /locus_tag="RHV13_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV1242812.1"
                     /translation="MLRATKVRIYPTPEQAEFINAQFGAVRFAYNKALHIKKHSYQRH
                     RVNLNPRKDLKPLLAVAKKSRRYGWLKEYDSIALQQAVINLDVAFSNFFNPKLKAGKP
                     TFKSKHGRQSSYHCVGIKVLDGAIKIPKILPIEARLHREITGMLKSITLSRSATGKYY
                     ASLLCDDGMEAPAKPALISNVTGLDMGLAHFVIKSDGNKIASPRHLINASRNLRRKQK
                     ALSRKKKGSANRRKARIQLAAVHERVASARADFQHKLSRAIVDENQAVIVETLKTANM
                     MKNHHLARAIGDAGWSGFITKLEYKATEKGVHLVKLDQWFASSKICHCCGHKMSEMPL
                     HKRIWQCPECGVEHDRDINAALNIKRKGILELQAAGLVVSAHGGQRKSVTQTVAA"
     gene            complement(4514..5761)
                     /locus_tag="RHV13_003306"
     CDS             complement(4514..5761)
                     /locus_tag="RHV13_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HDV1242813.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSINPHINFWLVSLFGAVFMMINMRFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEAVCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(5833..6747)
                     /locus_tag="RHV13_003307"
     CDS             complement(5833..6747)
                     /locus_tag="RHV13_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HDV1242814.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGI
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDRDICALAKEYEIAMLLV
                     TKGAEGGVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            6963..8396
                     /gene="cscA"
                     /locus_tag="RHV13_003308"
     CDS             6963..8396
                     /gene="cscA"
                     /locus_tag="RHV13_003308"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDV1242815.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADSWWMVVGAKDQGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFVAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSISPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVVQHGALWQLG"
     gene            complement(8404..9462)
                     /locus_tag="RHV13_003309"
     CDS             complement(8404..9462)
                     /locus_tag="RHV13_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDV1242816.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGSVTRIPCPLLIRCST"
     gene            9642..9860
                     /locus_tag="RHV13_003310"
                     /pseudo
     CDS             9642..9860
                     /locus_tag="RHV13_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            9878..11206
                     /gene="dsdA"
                     /locus_tag="RHV13_003311"
     CDS             9878..11206
                     /gene="dsdA"
                     /locus_tag="RHV13_003311"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HDV1242817.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLHNATHLVWATGGGMVPEEEMEQYLAKGR"
     gene            complement(11314..12852)
                     /gene="emrY"
                     /locus_tag="RHV13_003312"
     CDS             complement(11314..12852)
                     /gene="emrY"
                     /locus_tag="RHV13_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HDV1242818.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(12852..>13894)
                     /gene="emrK"
                     /locus_tag="RHV13_003313"
     CDS             complement(12852..>13894)
                     /gene="emrK"
                     /locus_tag="RHV13_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HDV1242819.1"
                     /translation="MISTDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSL
                     DKTDATIALNKAKNNLANIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLA
                     KQGVISKETLEHTKDTLISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKE
                     AWLALKRTDIKSPVTGYIAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDV
                     RIGQSVNIISDLYGENVVFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVS
                     LDPKELMEHPLRIGLSMTATIDTKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEK
                     EISNIISHNGQL"
BASE COUNT         3900 a         3381 c         3230 g         3383 t
ORIGIN      
        1 gtccgggtca ttgtcggttt atctcctttt acttgtggta attgagggat aacaacaaaa
       61 aaggtgagta ttgcgactca ccttttttat ttgtttctta ttcagaatcg atatctttta
      121 aatcatcctg aatcgcttgc gcgttcggat tttcctgcgg tttgagttcg ccgccattag
      181 cgatgaaatc atgacgctgg aagtacgctt cgcgcaccat aatataagga tcggacgact
      241 gacgcagcag accgtcggaa tccaacaact gcgcacgagt ttctatccct tcaagcgtcc
      301 atttacccac agacatcggc caggtcagcc aggatagaac cgggtaaaga ccatccgcca
      361 tatcaccacc gtcatcacgc agcgtgaagc taccgtagaa cggtaactgg acgtaaggcc
      421 cataacccac gccataatga ccaagcgtac taccgaagcg gtgaggttca gtccgttgca
      481 gtttcgggtt cgccatccct gcaacatcaa taaaaccacc catccccaaa atggtgttca
      541 ggaaaaagcg ggtaaagtgg accatcccct gataagggtc gccctgcaag aagtagttca
      601 ccatcaccgc gggttcttca aggttgccgg taaagttgct caaaccgtta cgcgccggtt
      661 gcggaacata atcacgccag gcgacagcga ccggtcgaac aatatacggg tctagtacgt
      721 tgaagttgaa gttgtacatg gtgcggttga acccttctaa cgggtcagaa cgcccttgct
      781 gatctgtacc ggaactcgca caccccacca gaagcgtagt tcccagagca agcgccgaca
      841 ggcgaagctt cataaatgtc tccctgtttt tttatggctt atgcagtttg ccatccatga
      901 cggaacgata ccgtatccgc ctgtttaggt gtgggcgatt gtaacagcac gtcaactgat
      961 gtccagacgc cctgatttgc tgatttgatc atagcctggt aatcgccgcc ctgtaggcta
     1021 cttgattcta tggaaacaga aaaaggcaaa cgcggccttt tctacatttt cagagtaact
     1081 cccgccagtt gcgagcaaaa aagccgctac gctttagcta tacgtgctaa ttcaagagaa
     1141 gagaccatgg acaacgacaa aattgatcaa cacagcgacg aaattgaagt tgagagcgaa
     1201 gaaaaagagc gtggcaaaaa aatagaaata gatgaagatc gactcccctc cagggcgatg
     1261 gcaattcatg agcatatccg ccaggatggt gaaaaagagc tggaacgcga cgcaatggca
     1321 ctactgtggt cagccattgc ggcgggtctg tcgatgggcg cttcattact ggcaaaaggg
     1381 atatttcatg tcgaactgga aggtgtgccg ggcagcttct tgctggagaa tctaggttat
     1441 acctttggtt ttattatcgt cattatggcc cgccagcaat tatttaccga aaacaccgtg
     1501 actgcggtac tacccgtcat gcaaaaaccg acaatgagca acgttggctt acttatgcgg
     1561 ttatggggcg tcgtgctgct gggtaatatt ctcgggacag gtattgcggc gtgggcattt
     1621 gaatatatgc ctatcttcaa tgaagaaact cgcgatgcat ttgtcaaaat cggcatggat
     1681 gtgatgaaga acacccccag cgagatgttt gccaacgcga tcatttccgg ctggctgatc
     1741 gccactatgg tatggatgtt tcctgcagcg ggtgcggcaa agattgtggt gattatattg
     1801 atgacctggc ttattgccct gggtgacacc actcacatcg ttgtcggttc tgttgaaatc
     1861 ctctatctgg tgtttaacgg tacgctgcac tggagcgatt tcatctggcc cttcgcacta
     1921 cctactttag cggggaacat ctgcggcggc acctttatct tcgcgttaat gagtcatgca
     1981 cagatccgta acgacatgag caacaagcgc aaagcagaag cacgtcaaaa agcagaacgt
     2041 gcggaaaaca ttaagaaaaa tgataaaaac ccggcataaa tggcgagggt ttaagcaatc
     2101 gagcggcagc gtacttaccc cgcagtccat tagcgggtat actcatgccg cattgtcctc
     2161 ttagttaaat ggatataacg agcccctcct aagggctaat tgcaggttcg attcctgcag
     2221 gggacaccat ttatcagttc gctcccatcc gtaccagtcc gcaaaatccc ttgaatatca
     2281 agccttccgt agattcacag ttcgacatgg ttcgcgtcag atcgttgaca gccgcacacc
     2341 atgacgggta aaaagtggat aaaataattt tacccaccgg atttttaccc atgctcaccg
     2401 ttaagcagat tgaagcagca aagccgaaag aaaaaccata ccgccttctc gatggtaatg
     2461 gcctgtacct ttatgtccct gtgtcaggga aaaaggtatg gcagcttcgc tacaagattg
     2521 acggtaagga gaaaatcctg accgtaggaa aatatccgct aatgactttg caggaggcaa
     2581 gggataaggc atggactgcg aggaaagaca tctcggttgg catcgatccg gtaaaggcga
     2641 aaaaggcttc gtctaacaac aattccttta gcgaaaaacg ccgtaaaccc tcgcccaatg
     2701 cgggcgggga tataaggcga aaagcccgca gggctttaaa gatcaatcag gtgttgactg
     2761 actctgaacg taggctctga gcgtttcaat cgttgcccct ccggtgctac agacaaagta
     2821 cgatctcgac cacaaaagcc ccgttttact ctgcatacgt aaatgcgcgt tctgctggcg
     2881 tagcaaacgt gatgataccg attttaaatt attgaccatc acactgaccg ccagttttgg
     2941 cgggtaggca accagcaggt gtacatggtc tggctccccg tccatctcaa taatttcgca
     3001 ttcaagcttt gccgcagcgg agccaaaagc ctcacgcaac tgagcgatca tctgactgtc
     3061 gaatagtttg cgtcgatact ttgtcgtaaa aataaggtgc acaaccagct tactgacact
     3121 gtgtcgtttg cggagaaatc ccgccagtaa atcatcatga ttactcaatt gatatattca
     3181 cctaaagcgt taaaataaaa gaattatatc aatgagcgct gattatgtta agagcaacaa
     3241 aagtacgcat ctacccgaca ccagaacagg ctgaatttat caacgcccag ttcggtgcag
     3301 tgcgttttgc gtacaacaaa gctctgcata tcaagaaaca ttcttaccag cggcaccgcg
     3361 taaatttaaa cccgcgtaaa gacctcaaac cactgcttgc agtggcgaag aaatcccgcc
     3421 ggtatggatg gcttaaagaa tatgattcta ttgcgttgca gcaggcggta attaacctcg
     3481 acgtggcttt ttctaacttt ttcaatccga aactgaaagc aggtaaacct acctttaaaa
     3541 gtaaacacgg cagacaatcg agctatcatt gtgttggcat aaaagtcctt gatggtgcga
     3601 taaaaatacc gaaaatcttg ccgattgaag cgcgtttaca tcgtgaaatt acgggaatgc
     3661 tgaaaagtat cacgttgagc cgcagcgcaa ccgggaaata ctacgcgtcg ttactctgtg
     3721 acgacgggat ggaagcgccg gcaaagcccg cactgatatc aaatgtgaca gggctggata
     3781 tggggctggc gcattttgtc atcaaatcgg acggcaataa aattgccagt ccccgccacc
     3841 taatcaatgc aagtcgcaac cttcgccgga agcagaaagc actgtcccgt aagaaaaaag
     3901 ggagcgcaaa caggcgcaaa gccagaatac aacttgcagc cgttcacgaa cgggtagcca
     3961 gtgcccgtgc cgatttccag cacaagttat ctcgcgcaat tgttgacgaa aaccaagcgg
     4021 taattgtcga gacattgaaa acggcaaata tgatgaaaaa tcaccatctg gctcgtgcga
     4081 taggcgatgc tggatggtct ggattcatca caaaactgga atacaaagca acagagaaag
     4141 gtgtgcatct ggtgaaactg gatcaatggt tcgccagttc gaaaatctgc cactgctgcg
     4201 gtcacaaaat gtcggagatg ccattacata agcgcatctg gcagtgtcca gagtgtggag
     4261 ttgaacatga ccgcgatatc aacgcggcgc tcaacatcaa acgcaagggc atactggaat
     4321 tacaagcggc gggactcgtc gtttctgccc atggaggcca gcgtaaatcc gtcacacaga
     4381 cggttgcagc ttaagaagtg ggaagcctcg cccgacaggg cggggagcag tcaccagggc
     4441 taagcatttt actattatta ttttccggtt gagggatatg gagatatcga caacaaccgg
     4501 aaaaagttta cgtctatatt gctgaaggta caggcgtttc cataactatt tgctcgcgtt
     4561 ttttactcag gaagaaaatg ccaaatagca acatcaggca gacaataccc gaaattgcga
     4621 agaaaactgt ctggtagcct gcgtggtcaa agagtatccc agtcggcgtt gaaagcagca
     4681 caatcccaag cgaactggca atttgaaaac caatcagaaa gatcgtcgac gacaggcgct
     4741 tatcaaagtt tgccacgctg tatttgaaga cggatatgac acaaagtgga acctcaatgg
     4801 catgtaacag cttcactaat gaaataatcc aggggttaac gaacagcgcg caggaaagga
     4861 tacgcaacgc cataatcaca acaccgataa gtaatgcatt ttttggccct acccgattca
     4921 caaagaaagg aataatcgcc atgcacactg cttcgagtac cacctggaat gagttgagat
     4981 aaccatacag gcgcgttcct acatcgtgtg attcgaataa acctgcataa aagacaggaa
     5041 aaagttgttg atcaaaaatg ttatagaaag accacgtccc cacaataaat atgacgaaaa
     5101 cccagaagtt tcgatccttg aaaactgcga taaaatcctc tttttttacc cctcccgcat
     5161 ccgccgctac gcactggtga tccttatctt taaaacgcat gttgatcatc ataaatacag
     5221 cgccaaatag cgagaccaac cagaagttga tatggggatt gatactaaaa aatatgccgg
     5281 caaagaacgc gccaatagca tagccaaaag atccccaggc gcgcgctgtt ccatattcga
     5341 aatgaaaatt tcgcgccatt ttttcggtga agctgtcaag caaaccgcat cccgccagat
     5401 accccaagcc aaaaaagagc gcccccagaa ttagacctac agaaaaattg ctttgcagta
     5461 acggttcata aacgtaaatc ataaacggtc cggtcaagac caggatgaaa ctcatacacc
     5521 agatgagtgg tttcttcaga ccgagtttat cctgaacgat gccgtagaac atcataaata
     5581 gaatgctggt aaactggttg accgaataaa gtgtacctaa ttccgtccct gtcaacccta
     5641 gatgtccttt cagccaaata gcgtataacg accaccacag cgaccaggaa ataaaaaaga
     5701 gaaatgagta actggatgca aaacgatagt acgcatttct gaatgggata ttcagtgcca
     5761 taattacctg cctgtcgtta aaaaattcac gtcctattta gagataagag cgacttcgcc
     5821 gtttacttct cactattcca gttcttgtcg acatggcagc gctgtcattg cccctttcgc
     5881 cgttactgca agcgctccgc aacgttgagc gagatcgata attcgtcgca tttctctctc
     5941 atctgtagat aatcccgtag aggacagacc tgtgagtaac ccggcaacga acgcatctcc
     6001 cgcccccgtg ctatcgacac aattcacaga cattccagca aaatggtgaa cttgtcctcg
     6061 ataacagacc accccccctt ctgcaccttt agtcaccaac agcatggcga tctcatactc
     6121 ttttgccagg gcgcatatat cccgatcgtt ctgtgttttt ccactgatta gtcgccattc
     6181 ttcttccgag agtttgacga catccgccag ttgtagcgcc tgccgcaaac acaagcggag
     6241 caaatgctcg tcttgccata gatcttcacg aatattggga tcgaagctga caaaacctcc
     6301 ggcatgccgg atcgccgtca tcgcagtaaa tgcgctggta cgcgaaggct cggcagacaa
     6361 cgcaattgaa cagagatgta accattcgcc atgtcgccag cagggcaagt ctgtcgtctc
     6421 taaaaaaaga tcggcactgg ggcgaaccat aaacgtaaat gaacgttccc cttgatcgtt
     6481 cagatcgaca agcaccgtgg atgtccggtg ccattcatct tgcttcagat acgtgatatc
     6541 gactccctca gttagcagag ttctttgcat taacgcacca aaaggatcat caccgacccg
     6601 acctataaac ccacttattc cgcctaatct ggcgattccc accgcaacgt tagctggcgc
     6661 gccgccagga caaggcagta ggcgcccgtc tgattctggc aagagatcta ctaccgcatc
     6721 ccctaaaacc catactttgg ctgacatttt tttcccttaa attcatctga cttacgcata
     6781 gtgataaacc tctttttcgc aaaatcgtca tggatttact aaaacatgca tattcgatca
     6841 caaaatgtca tagttaacgt taacatttgt gatattcatc gcatttatga aagtaaggga
     6901 ctttattttt ataaaagtta acgttaacaa ttcaccaaat ttgcttaacc aggatgatta
     6961 aaatgacgca atctcgattg catgcggcgc aaaacgcact agcaaaactt catgagcacc
     7021 ggggtaacac tttctatccc cattttcacc tcgcgcctcc tgccgggtgg atgaacgatc
     7081 caaacggcct gatctggttt aacgatcgtt atcacgcgtt ttatcagcat cacccaatga
     7141 gcgaacactg ggggccaatg cactggggac atgccaccag cgacgatatg atccactggc
     7201 agcatgagcc tattgcgcta gcgccaggag acgataatga caaagatggg tgtttttcag
     7261 gtagtgctgt cgatgacaat ggtgtcctct cacttatcta caccggacac gtctggctcg
     7321 atggtgcagg taatgacgat gcaattcgcg aagtacaatg tctggctacc agtcgggatg
     7381 gtattcattt cgagaaacag ggtgtgatcc tcactccacc agaaggaatc atgcacttcc
     7441 gcgatcctaa agtgtggcgt gaagccgact catggtggat ggtagtcggg gcgaaagacc
     7501 aaggcaacac ggggcagatc ctgctttatc gcggcagttc attacgtgaa tggactttcg
     7561 atcgcgtact ggcccacgct gatgcgggtg aaagctatat gtgggaatgt ccggactttt
     7621 tcagccttgg cgatcagcat tatctgatgt tttccccgca gggaatgaat gccgagggat
     7681 acagttaccg aaatcgcttt caaagtggcg taatacccgg aatgtggtcg ccaggacgac
     7741 tttttgcaca atccgggcat tttactgaac ttgataacgg gcatgacttt tatgcaccac
     7801 aaagctttgt agcgaaggat ggtcggcgta ttgttatcgg ctggatggat atgtgggaat
     7861 cgccaatgcc ctcaaaacgt gaaggctggg caggctgcat gacgctggcg cgcgagctat
     7921 cagagagcaa tggcaaactc ctacaacgcc cggtacacga agctgagtcg ttacgccagc
     7981 agcatcaatc tatctctccc cgcacaatca gcaataaata tgttttgcag gaaaacgcgc
     8041 aagcagttga gattcagttg cagtgggcgc tgaagaacag tgatgccgaa cattacggat
     8101 tacagctcgg cactggaatg cggctgtata ttgataacca atctgagcga cttgttttgt
     8161 ggcggtatta cccacacgag aatttagacg gctaccgtag tattcccctc ccgcagcgtg
     8221 acacgctcgc cctaaggata tttatcgata catcatccgt ggaagtattt attaacgacg
     8281 gggaagcggt gatgagtagt cgaatctatc cgcagccaga agaacgggaa ctgtcgcttt
     8341 atgcctccca cggagtggct gtggtgcaac atggggcact ctggcaactg ggttagcata
     8401 atatcaggtg gaacaacgga tcaacagcgg gcaagggatc cgcgtcacac ttcccccttc
     8461 acgaccttca ataatatgca atgcagcttc ccgcccgata atgtcatgtg gaagctgaat
     8521 tgtggtcagc ggcggtaaaa acagatgccc gacgccaacc agattatcaa agcccattac
     8581 ggcgacatcc tgcgggatac gtaccccctt cgccaggaga acctgataag ccacaaaggc
     8641 tgcgcgatcg ttaccacata tcagaacatc aaaatctggt ttgcccgatt tgaagtgggc
     8701 attgagtaaa cttgcgagat cggtgtagtg atcatcacct gttgccatgt gaaattgttt
     8761 cacctcagcc agatctcgtc cagcatcacg ccaggcctgc tcaaatccct gccgacgata
     8821 ccctgttgcc aacgcacttt ccggtagcca gaagcataac ggttgacgat agcccgccgc
     8881 gagcaaatgc tgtgttgatt catattgtgc agtgtaatca tcagggatat aactgggtaa
     8941 cgctgggtca tccgccacac agttcgccaa tacaatattt tcaccataca gagactcagg
     9001 cagcgtgata tgtcgcagcc ccattgtagt atagataatg ccatccggac ggtgggcaag
     9061 cagctgacgt gccgcgcggg cagcgtcatc ttcagaaaaa atattgatta aaaaactatt
     9121 ccagccgaac tcgctggcgg tttgctcaat ggcaagcaga atatcaacag agaaaggagt
     9181 ggtagccgtg tcctgcgcca gcacggcgag agtcgacggc ttacgtcctt gagcgcgcat
     9241 cttacgggcg gaaagatcag gaacataatt cagggtctgg attgcctgca atacgcggtc
     9301 acgcgttgca ggacgcacag attctgcatt atgcatcacc cgggagactg tcatcatcga
     9361 cactcccgcc aggcgtgcga catcctttaa tgaagccata cccaagccgt ttgccgtaaa
     9421 acgggcactg tagcagaaac agacgtcact ggcgagatcc aacgaaattc tcaacgccct
     9481 atcacccgac acggcaatac aataaaaaat aacaataatt gccggacaat tgtcccaaat
     9541 tccgcctctg ttctcgccaa cgagtaacca tataggcaag taagtaaaac atgccaactg
     9601 gcatcgccaa cctggagaag aaaatataaa tatggatcat tgtgctgccg ctgtatcccg
     9661 acatcagccc ggaaattatt gcgattgcta tcggttcagg cgcgattggc tgcacgatcg
     9721 ttatggactc gcttttctgg ctggtgaagc aatattgcgg cgctacgctc aatgaaacat
     9781 ttaaatacta tacgacagcg acatttatcg cttcagtcgt cgctctggcg ggcacattcc
     9841 tgctgtcatt tatcatctaa gcgcaaagag acgtactatg gaaaacgcta aaatgaactc
     9901 gctcatcgcc cagtatccgt tggtaaagga tctggttgct cttaaagaaa ccacctggtt
     9961 taatcctggc acgacctcat tggctgaagg tttaccttat gttggcctga ccgaacagga
    10021 tgttcaggac gcccatgcgc gcttatcccg ttttgcaccc tatctggcaa aagcatttcc
    10081 tgaaactgct gccactgggg ggattattga atcagaactg gttgccattc cggctatgca
    10141 aaaacggctg gaaaaggaat atcaacaacc aatcagcggg caactgttac tgaaaaaaga
    10201 tagccatttg cccatttccg gctccataaa agcacgcggc gggatttatg aagtcctggc
    10261 acacgcagaa aaactggctc tggaagcggg gttgctgacg cttgatgatg actacagcaa
    10321 actgctttct ccggagttta aacagttctt tagccaatac agcattgctg tgggctcaac
    10381 cggaaatctg gggttatcaa tcggcattat gagcgcccgc attggcttta aggtgacagt
    10441 gcatatgtct gctgatgccc gggcatggaa aaaagcgaaa ctgcgcagcc atggcgttac
    10501 ggtcgtggaa tatgagcaag attatggtgt tgccgtcgag gaaggacgta aagcagcgca
    10561 gtctgacccg aactgtttct ttattgatga cgaaaattcc cgcacgttgt tccttgggta
    10621 ttccgtcgct ggccagcgtc ttaaagcgca atttgcccag caaggccgta tcgtcgatgc
    10681 tgataaccct ctgtttgtct atctgccgtg tggtgttggc ggtggtcctg gtggcgtcgc
    10741 attcgggctt aaactggcgt ttggcgatca tgttcactgc ttttttgccg aaccaacgca
    10801 ctccccttgt atgttgttag gcgtccatac aggattacac gatcagattt ctgttcagga
    10861 tattggtatc gacaacctta ccgcagcgga tggccttgct gttggtcgcg catcaggctt
    10921 tgtcgggcgg gcaatggagc gtctgctgga tggcttctat acccttagcg atcaaaccat
    10981 gtatgacatg cttggctggc tggcgcagga agaaggtatt cgtcttgaac cttcggcact
    11041 ggcgggtatg gccggacctc agcgcgtgtg tgcatcagta agttaccaac agatgcacgg
    11101 tttcagcgca gaacaactgc ataatgccac tcatctggtg tgggcgacgg gaggtggaat
    11161 ggtgccggaa gaagagatgg agcaatatct ggcaaaaggc cgctaattaa ttttcaattc
    11221 agcatcgcca tcctttccct gggtgagcga tgctgccgat ggcacagact taagatcccc
    11281 ggtcttaccc gctataaccc cctcctttaa ccatcaccca acgcctttcg ctgtaaacgg
    11341 cggtttcgca aaccaaacca aaaccgtaag taagataaaa gcaatagccg ccatacggaa
    11401 aatctcattt gcagaaatag aaagtgactg ctgggttatt tcattattaa tttcattaag
    11461 aactcctgaa agcgaaccat agtatttatc cataatttgt gacgatgaat taaacacagg
    11521 gttaaattga tcgatggttg ctgtcaactg actatggtgt aacgattcgc gtcgccccca
    11581 cagcgtcatt gtcaacgatg taccaactga tcctgacaag gtacgaaaaa aattactcat
    11641 actcgaggca ttggcaaatt tattatctgg caaccctgaa aacgaaatcg ttgttaaggg
    11701 taaaaagaaa caggcaacgg cgaatccctg gaaaaactgt ggcaaaatga tgcctgtaaa
    11761 atcaatcgtt ggcataaatg tcacagaacg ccagtaatag caaaccgcat acatcaaaaa
    11821 actaaatgtc actaacaacc gcatgtctat tttgttgcca taacgtccta tcaaaggtga
    11881 aattaatagt ggcatgatgc cgatgggcgc ataagcaagt ccggcccata tcgcattata
    11941 ccccatcgtt tcctggagta actgcggcat aaggacgatc gctccagagt aaaataaata
    12001 cgcgcatgtg atactcacaa taccaatagt gaagttacgg gacttaaaca aactgagatc
    12061 aagaatcggg ttctctgagg tcgactccca aatgactaaa gagatcagag aaataactga
    12121 tactactgtt aatattatta ttgtactcga gttgaaccaa tccagatcgc gccctttatc
    12181 aagcataatt tgcaagccac caacaccgag cactaacagg gtcagtcctg gtagattcat
    12241 tttgaccggt gaagtctcag tttctcttcc tttaagtaag gttaagcata atgtcaggac
    12301 gataataccc atagggacat tgattaaaaa tatccaaccc cagctaaagt tatcacaaat
    12361 ataaccgccc aatatcggcc cacatatcgg agcgataatc acggtcattg accataatgc
    12421 cagagcaaat gttctttttt ctggtggata attccttaat aataaactct gtgacagtgg
    12481 aattaacggc cctgccatta acccctgaac gactctaaaa aatatcagca catcaagatt
    12541 ggtcgataaa ctacacatta aggaagacag cgaaaaaaaa gtgactgaaa gtaaaaataa
    12601 ccttaattcg cctattcttt gtgccagcct gccggtaaca gggatcgcaa tggcatttgc
    12661 tacgccaaac gaggtgataa cccaggtgcc ttcgtctgtt gatgctccca gaaagccaga
    12721 tattgtcggt attgcgacgt tagaaatagt ggaatccaac atttgcataa acgtcgctaa
    12781 tgacaatgca atagtgacgc accataacgt cccaccggtt aatggtgccg gagttgattt
    12841 agtgattgcc attaaagttg tccattatgc gaaataatgt tgctaatttc tttttctatc
    12901 ggactggtat cgataactaa agccttactg gtataagccg gcatggacgt cacggttgaa
    12961 gccagctcag gcatctcagc aatgtcttcg ttcttcgtat caatagttgc agtcatcgat
    13021 aaaccaatgc gcaaggggtg ttccatgagt tcttttggat caagagaaac ttcaaccggt
    13081 acacgctgaa cgattttgat ccagttccct gtcgcatttt gtgcaggtaa taaggagaac
    13141 gcattgccgg ttcccatatt gatccctgtc acccggccat gaaacacaac attctcacca
    13201 taaagatcgc tgataatatt gaccgattga ccaatccgta catctgtgag ttgtgtttct
    13261 ttaaagttgg cattaaccca catttgacgt gccggtacga cagccattaa cgattgtccg
    13321 gggctcactg tttcgccgac ctgaacactt ctctgggcaa tatagccggt aaccggactc
    13381 ttaatatccg tacgtttaag cgccaaccag gcttctttag ttgcatccgc cgcttcaacg
    13441 acttgtggct gacggtttaa tggtgtgttc attactaaag ctttattcgc tttataagcc
    13501 tggatagcgg cattcaatgc cgctttgcta cttattaacg tatctttggt atgctccagc
    13561 gtttcttttg aaataacccc ctgcttcgct aaaggcacac gacggttata atcttctaaa
    13621 gattgttgat actgaatacg tgctgaagcg acttcggcac tgtattgttt atcctgtaag
    13681 tacagtttat tcgtttgccg aacaatattt gccagattat ttttagcttt attgagtgcg
    13741 atagtggcat cagttttatc cagtgaaact aaaatgtcac cctgtcgaac gtagttcgta
    13801 tctttatgat taactacagt gacactacct gagacttgtg cagaaattgg atctgcattc
    13861 cccgtgacat aggcgtcatc tgtactaatc atgt
//
LOCUS       DAPGOF010000124        13890 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.125, whole genome shotgun sequence.
ACCESSION   DAPGOF010000124 DAPGOF010000000
VERSION     DAPGOF010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13890
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.125"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(132..884)
                     /gene="yecC"
                     /locus_tag="RHV13_003314"
     CDS             complement(132..884)
                     /gene="yecC"
                     /locus_tag="RHV13_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HDV1242820.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPKQPRTRQFLEKFLLQ"
     gene            complement(881..1549)
                     /gene="tcyL"
                     /locus_tag="RHV13_003315"
     CDS             complement(881..1549)
                     /gene="tcyL"
                     /locus_tag="RHV13_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HDV1242821.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1564..2550)
                     /gene="dcyD"
                     /locus_tag="RHV13_003316"
     CDS             complement(1564..2550)
                     /gene="dcyD"
                     /locus_tag="RHV13_003316"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HDV1242822.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCEALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEVLLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(2655..3455)
                     /gene="tcyJ"
                     /locus_tag="RHV13_003317"
     CDS             complement(2655..3455)
                     /gene="tcyJ"
                     /locus_tag="RHV13_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV1242823.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(3543..4094)
                     /gene="fliZ"
                     /locus_tag="RHV13_003318"
     CDS             complement(3543..4094)
                     /gene="fliZ"
                     /locus_tag="RHV13_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HDV1242824.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQTEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSQPASDIY"
     gene            complement(4140..4859)
                     /gene="fliA"
                     /locus_tag="RHV13_003319"
     CDS             complement(4140..4859)
                     /gene="fliA"
                     /locus_tag="RHV13_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HDV1242825.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(5179..6858)
                     /locus_tag="RHV13_003320"
     CDS             complement(5179..6858)
                     /locus_tag="RHV13_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HDV1242826.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLSGFNVNGKGAVANAKATEADLTGAGFSQGAVDTNGNSTWTKSTTTNYS
                     AATTADLLSTIKDGSTVTYAGTDTGLGVAAAGNYTYDANSKSYSFNANGLTGANTATA
                     LKGYLGTGANTAKISIGGTEQEVNIAKDGTITDTNGDALYLDITGNLTKNYAGSPPAA
                     TLDNVLASATVNATIKFDSGMTVDYTAGTGANITGASISADDMAAKLSGKAYTVANGA
                     ESYDVAAVTGAVTTTAGNSPVYADADGKLTTSASNTVTQTYHEFANGNIYDDKGSSLY
                     KAADGSLTSEAKGKSEATADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTT
                     NLSEAQSRIQDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            7124..8530
                     /gene="fliD"
                     /locus_tag="RHV13_003321"
     CDS             7124..8530
                     /gene="fliD"
                     /locus_tag="RHV13_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HDV1242827.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSNYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            8555..8965
                     /gene="fliS"
                     /locus_tag="RHV13_003322"
     CDS             8555..8965
                     /gene="fliS"
                     /locus_tag="RHV13_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HDV1242828.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEDSKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            8965..9330
                     /gene="fliT"
                     /locus_tag="RHV13_003323"
     CDS             8965..9330
                     /gene="fliT"
                     /locus_tag="RHV13_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HDV1242829.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVEPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            9408..10895
                     /gene="amyA"
                     /locus_tag="RHV13_003324"
     CDS             9408..10895
                     /gene="amyA"
                     /locus_tag="RHV13_003324"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HDV1242830.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKVQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(10929..11342)
                     /locus_tag="RHV13_003325"
     CDS             complement(10929..11342)
                     /locus_tag="RHV13_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDV1242831.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            11529..12734
                     /gene="yedE"
                     /locus_tag="RHV13_003326"
     CDS             11529..12734
                     /gene="yedE"
                     /locus_tag="RHV13_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HDV1242832.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            12731..12964
                     /gene="yedF"
                     /locus_tag="RHV13_003327"
     CDS             12731..12964
                     /gene="yedF"
                     /locus_tag="RHV13_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HDV1242833.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            13071..13739
                     /locus_tag="RHV13_003328"
     CDS             13071..13739
                     /locus_tag="RHV13_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HDV1242834.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPISRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPFFIYRADGQPIFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAREWMRQEISGKEASEIAASGCVPANQFSWHPVSRAVGNVKNQGAAL
                     IQPV"
BASE COUNT         3374 a         3601 c         3442 g         3473 t
ORIGIN      
        1 tgaattactt ttgcaagcca tcgcattctc ttatgttatt aatgagttat gctgatttgt
       61 taagcagttt tatcacgctt gaaatggcgt cccgccccga caggtgaatc gtcggggctg
      121 attttttctt attattgcag cagaaacttc tcgaggaact ggcgggtgcg aggctgcttg
      181 ggatcggcaa ataacgcttt tgcggccccc tgctcgacta tccgcccctg gtccataaag
      241 atcgcccggt ccgcaacatc ccgggcaaag ctcatttcgt gtgtcacaat caccatcgtg
      301 cgcttttcct gcgccagctg acggatggtg ttcaggactt cgcccaccag ctctggatcc
      361 agtgctgacg ttggctcgtc aaacaaaatc acctcaggac gcattgccag cgcacgcgca
      421 atcgcaacac gctgctgttg accgccagac aaacgacgtg gatagctggt ttctttaccg
      481 gccagcccaa cttttgccag tagctcgcga gcgcgcgccg tggcctcttc tttcggttcg
      541 cctttgacga tcaccggccc ttcaataatg ttctccagca ccgtacgatg cggaaacaaa
      601 ttaaagttct ggaagacaaa cccgacgtgc tgacgcaact ggcgaatcag cgatttttgc
      661 tgacttaatg aacgtgcagt atcaatagtg atatcgccaa cggtgatcgt tcccgcttcc
      721 ggttgttcca gcagatttat gctacgtagc aacgtggttt tgccggaacc actcggaccg
      781 ataattgcca ccacttcgcc aggctttacc tcaaggtcga taccgtgcag caccgtctga
      841 ccgtgaaatt ttttcaccag gttcttaact tcaatggcac tcattttggt tctctctcct
      901 ggcgattaag ttgattctca aaatggttct gcaacgtcga taatactgtc gccatgatcc
      961 agtagatgag cgaagccgcc aggtacatgg tgaacacctc cagcgtgcgc gaggtaatca
     1021 actgcgcttg acggaacagc tccggcacct ggattgtcgc ggccagcgac gtatctttta
     1081 ccaggctgat aaagctgttc gacagcggcg gcaacgccac ccgcgccgcc tgtggcaaaa
     1141 tcgcgcgacg catggtctgc catggcgtca taccaatact ggcagctgct tcccactgac
     1201 ctttatcaat agaagaaatg gcggcgcgca gcgtttcggc agcataggcg gcagtgttca
     1261 gcgacagacc aatcattgct gacggaatgg gatctaattc gatgccaaac tgcggcaggc
     1321 cgtaatagat cataaacagt tgtgcgatga gcggcgtacc acgaaaaata gagatataaa
     1381 aacgcgccag ccagcgcacg ggccagattg gcgacaggcg catcagcgcg agaataaacc
     1441 cgagtaataa gccaaaaaac atgccgccga tactgagttg cagcgtatac ccagcccctt
     1501 tcagcaggaa cggcaaggaa tcaataacca gttgtatact ttcttgcatg agcgtttccg
     1561 gagctaaacg tggggatgat aggcgaacag cgcaggcgca ccgccggtat gaataaacag
     1621 aatcggccct tcatctttga agcgtttctg actgataccg tcaatcagac ccgccatcgc
     1681 ttttccggta tacacaggat caagcagaat gccttccagc cgcgccagca atttcactgc
     1741 ttccatgcct tcgtcgttcg gcacgccgta gccaggtgca aaatagtcat cccagagtaa
     1801 aacttctgct gatgcggtca gctccagttc tttcgcaatc gcctgttgta ggttaaccac
     1861 tttcggcaat tgatcggcaa cggaacgcga cacggtcacg ccaatcagtt cgctttcagg
     1921 catcaggtgt tccagcccaa cagccagtcc ggcgtgagtt ccggcactgc ccgatgcaac
     1981 caccaccgac gaaatattaa ccgccccttc acactgttgc gcgatttcca gcgcactctc
     2041 cacataacct agcgcgccca gagcattaga accgccaacc ggaatgacat acgggcgaaa
     2101 gccttgtgct tcgactcgcg tcgccagctc ttccagttgg gcattgggat cggtcagtgc
     2161 ttcgcacatt tcaatctggg tattgaacag atccagcaac aagcgattgc cgttggttaa
     2221 atagttttct gcggttgtgc caataggatt ttccagcagc gccacacagt gcaaaccgag
     2281 tttcgccgca actgcggcag tctgccgcac atggttagac tggatcgccc cggcagtaat
     2341 cagcgtatcg gcaccttcgc gcagagcatc tgccgcgaga aattctagct tacgtaattt
     2401 attgccgccc attgccatgg gggtgacgtc atcccgtttg atgaaaattt cccgtcctag
     2461 ataatcagaa aagcgcggca gatattcgag cggcgttggc gcgccgataa actccaaccg
     2521 tggaaaacgg gttaaattat gcagtggcat aacagcctcc gatgtgtgtt gttgtgattt
     2581 tcttattatg cacgctgaaa acgcgtaaat aaaaaaggcg ctagtgaaag cgcccttttt
     2641 tgtcattatg ctgattattt ggtcacatca gcaccaaacc atttttcgga aagagcttgc
     2701 agagtgccgt ctttttgcat ttccgcaatt gcatcattca ctgctttcag caggtcctca
     2761 tttcctttac gcagcgccac gccagactcc tgacgggaga atgcttcacc ggttactgcc
     2821 agcgtatcgt tggttttctt caccagatcc agcgccgcca gacggtcaac gaggatcgca
     2881 tcgatacggc ctacgcgcag atcctgatat ttggtcgggt catcatcata ggtacgcaca
     2941 tcgacgccct gaacattctg ccgcagccac tcttcatagt tggtgcccag accgacaccc
     3001 acttttttgc ctttcagatc agcagccgtt ttaatggtgc cttcgttacc ttttttcacc
     3061 agcgcctgaa taccagaaat ggtgtacggg gtagagaaat cgtatttttt cttgcgctca
     3121 tcagaaatgg tgacctgatt aatcaccaca tcaatacgtt tagagtccag cgacgccagc
     3181 ataccgtccc atttggtcgg ttttagtgac gcctcaacgc caagatgttt tgccagctgt
     3241 tgggcaaatt ccacttcaaa accggttaat ttgccgtcat ccccctgaaa actgaacggc
     3301 ggataagttc cttccagccc taccagtagc gtaccgcgct ctttgacttt attaagcaga
     3361 ccttcatctg caaaactttt aacgctcatg cccgcaacca gcgccacggc catcacaccc
     3421 atcaatgcct gacgtcccag atgtgctaat ttcatattca ccccgaatgt tgttatgtct
     3481 gtttgcagtg tagagccatt gtttgtaaac acaaaaacaa ctccgctaca tcttattctt
     3541 atttaatata tatcagaagc aggctggctg gaggatttct gcacaagtcc ggtacacgtt
     3601 tgacgctgat agtgttgata cttccgtaac gcaatgcggt aattgttggt gctggtggta
     3661 ggcagccacg gggcgaggat ttcatcaagg aaaccgtcct gcagctgatc gagggaaata
     3721 ttttgctcgt gtagatgatt ccccaaacgg cgcaaacgaa cgacatattc acgcaccgta
     3781 ccagggctca tttcagtttg ctcaaaaaga aattgcttaa agccgataat atcgaaaaaa
     3841 tcactctgcg ttttgcagtg taaatcaccg caaaatcggc acaatgccgc ccatgatttt
     3901 tgttccgttt gccagccatt ttcatcaagc agcgtgtcca ggcgggaaat ctcgatttta
     3961 ttcactattt tgccgtcgcg aactaaggta atgcgatcga gtaatttacg gcaatgcgcg
     4021 caatgggtct ggctgtgttt aaagtcttta agatagcggc ttaatggccg tcttttcagg
     4081 tgctgcacca tcattaagaa ctcctggtag tcaaagttaa agtgcggcat ttactgacgt
     4141 tataacttac ccagtttagt gcgtaaccgt ttaatagcct ggctgtgtaa ctgactgacc
     4201 cgcgattccc cgacctccag cactgcacca atctctttga gattcagctc ttcctggtaa
     4261 tagagggtta ataccagttt ttcgcgctcc ggcaacgttt cgatggcttc catcacccgc
     4321 tggcgcaaat tactgtccag cagttgttgt agcgggtttt ctcgctgatg atcatcagta
     4381 accagttcga tgctatcgcc gtgctcttcg cgccactcat cgtaggagaa gagctggctg
     4441 ttattggtgt cgagcaacat ttggcgataa tcggcaatat ctatccctaa acgttccgct
     4501 acctcagttt ccgtggcgtt gcggccaagt tcctgctcca gttgccctat tgcctgtgcc
     4561 acttcacgcg cgttgcgacg cacgctgcgc ggcacccagt cacggctgcg aagttcatcc
     4621 agcatagcgc cacggatacg ctgcactgcg taagttgtaa atgccgttcc ttgtagggcg
     4681 tcatagcgtt cgacggcatt aagtaaccca atgccgcccg cctgtagcag atcgtcaagt
     4741 tccacgctcg cgggcagtcg aacctgcagg cgcaatgctt cgtgacgcac cagcgggaca
     4801 taacgctgcc acagcgagtg tttatccatt acaccttcag cggtatagag tgaattcacg
     4861 ataaacagcc ctgcgttata tgagttatcg gcatgattat ccgtttctgc agggttttta
     4921 atcggacgat tagtgggtga aatgaggggt tatttggggg ttacaggtaa attccaggca
     4981 gaaaaaaccc cgccggtggc ggggaagcac gttgctgaca aattgcgctt tatgttgccg
     5041 gatgcggcgt aaacgcctta tccggcctac aaaaatgagc aaattcaata atttgcaatt
     5101 caccttgtag gcctgataag cgcagcgcat caggcaattt ggcgttgccg tcagtctcag
     5161 ttaatcagtt tacgacgatt aaccctgcag cagagacaga acctgctgcg gtacctggtt
     5221 agcttttgcc agcacggagt taccggcctg ctgaatgatc tgcgctttcg acatattgga
     5281 cacttcggtc gcatagtcgg cgtcctgaat acgggactgc gcttcggaca ggttggtagt
     5341 ggtgttgttc aggttggtga ctgcggaatc cagacggttc tgcaccgcac cgagggagga
     5401 gcggaatttg tcgatggagc tgatcgcttc gtccagagct ttcaggggat cggcggttgc
     5461 ttcagatttc cctttagctt cagaagtcag agagccatct gcagctttat acagtgacga
     5521 gcctttgtca tcataaatgt taccattagc aaactcgtga taagtctgag taaccgtatt
     5581 actggcactc gtcgttaatt taccgtctgc atcggcatac acaggtgaat tacctgctgt
     5641 agttgttaca gcccccgtaa ctgcagcaac gtcataagac tcagcaccat tggcaacagt
     5701 gtacgccttt ccgctcagtt ttgcggccat gtcatctgca gaaatggatg cacctgtaat
     5761 attcgcgcca gtacctgcag tataatcaac cgtcataccg ctatcaaact tgatagtggc
     5821 atttacagtt gcggaagcta atacgttatc cagcgttgct gcaggtggtg aacccgcata
     5881 gttcttagtc aggttgccgg taatatccag atagagcgca tcaccattcg tatctgtaat
     5941 agtgccatct ttggcaatat tcacttcctg ctctgtacca ccgatagaaa ttttagcggt
     6001 gttagcacct gtccccaagt aacctttgag tgcagttgcg gtatttgcgc ccgtcagacc
     6061 attggcattg aaggaataag atttactgtt cgcatcataa gtataatttc ctgctgctgc
     6121 gacccccaat ccggtgtctg tccctgcata tgtaacagta gagccatcct taatggtcga
     6181 taacaagtca gcagttgttg cagctgagta attggtggtg gttgattttg tccaagtact
     6241 atttccgttt gtatccaccg ctccttgaga gaaaccagcc cccgttaaat ctgcttcggt
     6301 cgcttttgcg ttagccacag cccctttgcc attcacatta aatccactca aacccagagt
     6361 atcagagtca attttcttca gatcaatagt gatagtctgg ccgtcattcg caccaacctg
     6421 aattttcatc gaaccgtctt tcgccagtac gttcacaccg ttgaactggg tttgaccgga
     6481 tacgcggtca atttcgtcca gacgggattt gatttcgtcc tgaatggagt ccagatccga
     6541 atcagagtta gtcccggtag aagcctgaac cgtcagttca cggatacgct gtaagttgtt
     6601 gttgatttcg gacagcgcgc cttcggtggt ctgcgcaaca gaaataccgt cgttggcgtt
     6661 acgtgcagcc tgagtcaggc ctttaatgtt agaagtaaaa cggttagcaa tcgcctgacc
     6721 cgcggcgtca tccttcgcgc tgttgatacg caagccagaa gacagacgct cgatagaact
     6781 cgacagcgca gactggttct tgttgatatt attttgagtg atcagcgaga ggctgttggt
     6841 attaatgact tgtgccatga ttcgttatcc tatattgcaa gtcgttgatt acgtattggg
     6901 tttccacccg tcggctcaat cgccgtcaac cctgttatcg tctgtcgtaa aacaaccttt
     6961 agaatttttt tcacaaacaa ccattttttg ttagtcgccg aaatactctt ttctctgccc
     7021 cttattcccg ctattaaaaa aaacaattaa acgtaaactt tgcgcaattc aggccgataa
     7081 ccccggtatt cgttttacgt gtcgaaagat aaaaggaaat cgcatggcaa gtatttcatc
     7141 gctgggagtc gggtcaggcc tggatttaag ttcaatcctt gatagcctca ccgccgcgca
     7201 aaaagcgacg ctaaccccta tttcaaatca gcaatcgtcg tttaccgcta aacttagcgc
     7261 ctacggtacg ctgaaaagcg cgttgacgac tttccagacc gccaatactg cattgtctaa
     7321 agccgatctt ttttccgcca ccagcactac cagcagcacc accgcgttca gtgccaccac
     7381 cgcgggtaac gccatcgccg ggaaatacac catcagcgtc acccatctgg cgcaggcgca
     7441 aaccctgacc acgcgcacca ccagagatga tacgaaaacg gcgatcgcca ccagcgacag
     7501 taaactcacc attcaacaag gcggcgacaa agatccgatt accattgata tcagcgcggc
     7561 taactcgtcg ttaagcggga tccgtgatgc catcaacaac gcaaaagcgg gcgtaagtgc
     7621 gagcatcatt aacgtgggta acggtgaata tcgtctgtca gtcacatcaa atgacaccgg
     7681 ccttgataat gcgatgacac tctcggtcag cggtgatgat gcgctacaaa gttttatggg
     7741 ctatgacgcc agtgccagca gcaacggcat ggaggtctcg gtcgccgccc agaatgcgca
     7801 gctgacagtc aacaacgtcg ccatcgagaa cagcagcaac actatcagcg acgcgctgga
     7861 aaacatcacc ctgaacctga acgatgtcac cacgggcaac cagacgctga ccatcactca
     7921 ggacacctcc aaagcgcaaa cggcgattaa agactgggtg aatgcctata actcgctaat
     7981 agataccttc agcagcctga ccaaatacac cgccgtagat gcgggagctg atagccagag
     8041 ctctagcaat ggcgcactgc tcggcgactc cacgctgcgg acgattcaga cgcagttgaa
     8101 gtcgatgctg agtaataccg ttagttcttc caactataaa acgttggcgc agattggtat
     8161 cacgaccgat cccagcgatg gcaaactgga actggatgcc gacaaactca ccgctgcact
     8221 gaaaaaagat gccagcggcg taggtgcatt gattgttggc gatggtaaaa aaaccggcat
     8281 cacaaccacc atcggcagca acctgaccag ttggctttcg acaacgggca ttattaaagc
     8341 cgctaccgat ggcgttagta agaccctgaa taaattaact aaagactaca acgccgccag
     8401 cgatcgcatt gatgcgcagg tcgcgcgcta caaagaacaa tttacccaac tggacgtttt
     8461 aatgacctcg ttaaacagca caagcagcta cttaacgcag cagttcgaaa acaacagtaa
     8521 ttccaagtaa tcaatattca tcgggagaca ggtcatgtac gcggcaaaag gcacccaggc
     8581 ctatgcacaa attggcgtcg aaagcgccgt aatgagcgcc agccagcagc agctggtcac
     8641 catgctattt gatggagtgc tgagtgcact ggttagagcg cgcctgttta tgcaggacaa
     8701 caatcagcaa ggcaaaggcg tctctttgtc aaaagcgatc aacatcattg agaacggact
     8761 gcgggtgagt cttgatgaag atagcaaaga cgaattaacc caaaacttga ttgctcttta
     8821 tagctatatg gtcaggcgct tgctgcaagc caatttacgc aacgatgtct ccgcagtcga
     8881 agaggtggaa gcattaatgc gtaatattgc cgatgcctgg aaagagtctt tactctcccc
     8941 ttctttgatt caggacccag tctgatgaac aatgcaccgc atttgtattt cgcctggcaa
     9001 caactcgtcg aaaaaagcca gctcatgtta cgcctggcaa cagaagaaca gtgggacgaa
     9061 ctcatcgcca gcgaaatggc gtatgtgaat gcggtgcagg aaattgcaca cctgactgaa
     9121 gaggttgagc cgtccaccac gatgcaggag cagctccgcc cgatgctgcg cctgattctc
     9181 gacaacgaaa gcaaggtaaa gcagttatta cagattcgga tggatgaact ggcgaaactg
     9241 gtcggtcagt catcggtgca aaaatcggtg ttaagtgcct atggcgatca gggcggcttt
     9301 gtgctggctc cgcaggataa cctcttttga atctgaatga gtcgatggct cgcgaataat
     9361 ccgattacgg ctacgcttct aatgttcccc ttgaatggag tcgaagaatg cgtaatccca
     9421 cgctgttaca atgttttcac tggtattacc cggaaggcgg taagctctgg cctgaactgg
     9481 ccgagcgcgc cgacggtttt aatgatattg gtatcaatat ggtctggttg ccgcccgcct
     9541 ataaaggcgc atcgggcggg tattcggtcg gctacgactc ctatgattta tttgatttag
     9601 gtgagtttga tcagaaaggc agcatcccta ctaaatatgg cgataaagta caactgctgg
     9661 ccgccattga cgccctgaaa cgtaatgaca ttgcggtgct gttggacgtg gtagtcaacc
     9721 acaaaatggg cgcggatgaa aaagaagcta ttcgcgtgca gcgtgtaaat gctgacgacc
     9781 gtacgcaaat tgatgaagaa atcattgagt gcgaaggctg gacgcgttac accttccccg
     9841 cccgtgccgg gcaatactca cagtttatct gggatttcaa atgttttagc ggtatcgacc
     9901 atatcgaaaa ccctgatgaa gatggcatat ttaaaattgt taacgactac accggcgaag
     9961 gctggaacga tcaggttgat gatgaattag gtaatttcga ttatctgatg ggcgagaata
    10021 tcgattttcg caatcatgcc gtgacggaag agattaaata ctgggcgcgc tgggtgatgg
    10081 aacaaacgca atgcgacggt tttcgtcttg atgcggtcaa acatattcca gcctggtttt
    10141 ataaagagtg gatcgaacac gtacaggaag ttgcgccaaa gccgctgttt attgtggcgg
    10201 agtactggtc gcatgaagtt gataaactgc aaacgtatat tgatcaggtg gaaggcaaaa
    10261 ccatgctgtt cgatgcaccg ctgcagatga aattccatga agcatcgcgc atgggacgcg
    10321 actacgacat gacgcagatt ttcactggga cgctggtgga agccgatcct tttcacgccg
    10381 taaccctcgt tgccaaccat gacacccaac cgttgcaagc cctcgaagcg ccggtcgaac
    10441 cgtggtttaa accgctggcg tacgccttaa ttttgttgcg ggaaaatggc gttccttcgg
    10501 tattctatcc ggacctctac ggtgcgcatt acgaagatgt cggtggtgac gggcaaacct
    10561 atccgataga tatgccaata atcgaacagc ttgatgagtt aattctcgcc cgtcagcgtt
    10621 tcgcccacgg tgtacagacg ttatttttcg accatccgaa ctgcattgcc tttagccgca
    10681 gtggcactga cgaatatccc ggctgcgtgg tggtcatgtc gaacggggat gatggcgaaa
    10741 aaaccattca tctgggagag aattacggca ataaaacctg gcgtgatttt cttgggaacc
    10801 ggcaagagag tgtagtaacc gacgaaaacg gcgaagcgac cttcttttgc aacggcggca
    10861 gcgttagcgt gtgggttatc gaagaggtga tttaaattca tccccggcag caagccgggg
    10921 agatttcatt acggcagtgg cgtcggtaac ggcgctttat ccagcgcggc ggcacactct
    10981 gctgtcgggc gatccacacg ctccatcgtc ataccatcgt actcaatggt gttaccgtca
    11041 cgctcaacct cgtacagctc acgttttacg gtaacgttag tgagatcatc agacatcaat
    11101 gtcaacttgc ccggcacagc aatcacccgc tgccactgac ggcaatccag ggtatcgccc
    11161 tctttagtga caatcaggct gcctatcgct tccgggctga ccagcgcacg ttgcggccct
    11221 ttggtctgcc agtagcccgc cagccagtcc ggcgcggggg ttttcacgac attgttatag
    11281 ttttcgactt cggcacagcc cgccagcaac atgagcgcgc ctgcaattgc gagttttttc
    11341 atcattcgtc ctgcatgaga agaaaagagg attgtggcat taaagccctg atgtcgccag
    11401 cctttccagg ttcagagaga aatttgatct aactattatt tttgtgaatt attaccccga
    11461 aaataaattt gcatggtgta tgattcgcga ctcttgtttc aaaccttctg agttcagagg
    11521 ctacattcat gtcatggcag caattcaaac acgcctggtt gattaaattc tgggcgccca
    11581 tccccgcggt catcgcggcg ggtattctct ccacttacta ttttggcatt actggcacct
    11641 tttgggctgt cacgggtgaa tttacccgtt ggggcggcca gctcctgcaa ctgttcggcg
    11701 tccatgctga agagtggggt tactttaaaa ttatccatct ggaaggatcg ccattaaccc
    11761 gcatcgacgg gatgatgatc ctcggtatgt ttggcggctg ctttgccgca gcgctgtggg
    11821 ccaacaatgt caaactgcga atgccgcgca gccgtatccg cattatgcag gccatcattg
    11881 gcggcattat cgccggtttt ggcgcgcgtc tggcaatggg ctgtaacctg gcggcgttct
    11941 ttaccggtat tcctcagttc tcgctgcatg cctggttctt tgccatcgcc actgccattg
    12001 gttcatggtt tggcgcgcgc tttaccctgc tgcccatctt ccgtattccc gtgaaaatgc
    12061 agaaagtttc tgccgcctca ccgctgacgc aaaaaccgga tcaggcgcga cgtcgttttc
    12121 gtctcgggat gctggtcttt ttcggcatgc tgggctgggc gctgctcacg gcgatgaacc
    12181 aacccaaact ggggctggca atgctgtttg gcgtcggctt tggtttactg attgaacgtg
    12241 cgcaaatctg ctttacttcg gcgttccgcg atatgtggat caccggacgt actcatatgg
    12301 cgaaagcaat cattatcggt atggcggtga gtgccatcgg gatcttcagt tacgtacagt
    12361 taggcgttga acccaaaatc atgtgggcgg gaccaaacgc ggtaattggt ggtttactgt
    12421 ttggttttgg catcgtgctg gctggcggct gcgaaaccgg ctggatgtac cgcgcggtag
    12481 aaggccaggt gcactactgg tgggtcggtc tgggcaatgt gatcggctca acgattctgg
    12541 cgtattactg ggatgatttc gctccagcgc tggccaccga ctgggacaaa ataaacctgc
    12601 tgaaaacctt tggcccgatg ggtggcctgc tggtgacata tttgctgttg tttactgcgc
    12661 tgatgttgat tatcggctgg gaaaaacgct tcttccgccg tgcggcaccg cagactgcta
    12721 aggagatcgc atgaaaaata tcgttcctga ttaccgcctt gatatggtgg gtgaaccctg
    12781 cccttatccg gcagtcgcaa ctcttgaggc gatgccgcaa ttaaaaaaag gtgagatcct
    12841 ggaagtggtg agcgactgtc cgcagtcgat caataatatt ccactggatg cgcgtaatca
    12901 cggctatacg gtgctggata ttcagcaaga cgggccgacc attcgttatt taattcagaa
    12961 gtaatccctc attcccgctg tggcccacag cgggaacatc gttgcctctg acacatagat
    13021 tttacccgcc ccctctccta ttcttttcag tatgatctca ggaggtttct atgtgtggac
    13081 gctttgccca atcccaaacg cgtgaagatt accttgcgct tctcgcggaa gatattgaac
    13141 gcgatattcc ctacgatccc gaacccatta gcagatacaa cgtcgcgccg ggaaccaaag
    13201 ttcttctgct cagtgaacgt gatgaacacc ttcatctgga tccggttttc tggggatatg
    13261 ctccgggatg gtgggataaa ccgccgctga ttaacgcccg cgtagaaact gccgcaacca
    13321 gtcgcatgtt taaaccgctc tggcaacatg gtcgggcaat ctgttttgcc gatggctggt
    13381 ttgagtggaa aaaagaaggc gacaaaaaac agcctttttt tatctatcgt gctgatggac
    13441 aacctatttt tatggccgcg ataggtagca caccatttga gcgtggtgac gaagccgaag
    13501 gatttttgat cgtcactgct gcggcagatc aaggtctggt agatattcat gaccgccgcc
    13561 cactggtact gtcgccagaa gctgcgcggg aatggatgcg gcaagagatt agcggtaaag
    13621 aagcctcaga aatcgcggcc agtggctgtg ttccagcaaa ccagttctcc tggcacccgg
    13681 tatcgcgcgc ggtgggtaat gttaaaaacc agggcgcagc gttaattcaa cctgtttgat
    13741 aagcctggag attatctaca cctgcatgga catcattgta ctatgcccgt acatgcccca
    13801 tcacatcctc aacatcaaag tattagagcc ttgcaaacca ttatgcgtct cagttttgtc
    13861 tcatctatct tacgcaaaaa aatagaacta
//
LOCUS       DAPGOF010000125        13889 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.126, whole genome shotgun sequence.
ACCESSION   DAPGOF010000125 DAPGOF010000000
VERSION     DAPGOF010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13889
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.126"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..871)
                     /gene="yhaJ"
                     /locus_tag="RHV13_003329"
     CDS             complement(<1..871)
                     /gene="yhaJ"
                     /locus_tag="RHV13_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HDV1242835.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLRE"
     gene            976..1677
                     /locus_tag="RHV13_003330"
     CDS             976..1677
                     /locus_tag="RHV13_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HDV1242836.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1700..1864
                     /locus_tag="RHV13_003331"
     CDS             1700..1864
                     /locus_tag="RHV13_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242837.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2076..3386)
                     /locus_tag="RHV13_003332"
     CDS             complement(2076..3386)
                     /locus_tag="RHV13_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HDV1242838.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHDG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCARGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGDEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3414..4745)
                     /locus_tag="RHV13_003333"
     CDS             complement(3414..4745)
                     /locus_tag="RHV13_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HDV1242839.1"
                     /translation="MEITSNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(5020..6384)
                     /gene="tdcG"
                     /locus_tag="RHV13_003334"
     CDS             complement(5020..6384)
                     /gene="tdcG"
                     /locus_tag="RHV13_003334"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDV1242840.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDASFVRIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6456..6845)
                     /locus_tag="RHV13_003335"
     CDS             complement(6456..6845)
                     /locus_tag="RHV13_003335"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HDV1242841.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPS
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(6859..9153)
                     /gene="tdcE"
                     /locus_tag="RHV13_003336"
     CDS             complement(6859..9153)
                     /gene="tdcE"
                     /locus_tag="RHV13_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HDV1242842.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGIRYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9187..10395)
                     /gene="tdcD"
                     /locus_tag="RHV13_003337"
     CDS             complement(9187..10395)
                     /gene="tdcD"
                     /locus_tag="RHV13_003337"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HDV1242843.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVVIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10421..11752)
                     /gene="tdcC"
                     /locus_tag="RHV13_003338"
     CDS             complement(10421..11752)
                     /gene="tdcC"
                     /locus_tag="RHV13_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HDV1242844.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(11774..12763)
                     /gene="tdcB"
                     /locus_tag="RHV13_003339"
     CDS             complement(11774..12763)
                     /gene="tdcB"
                     /locus_tag="RHV13_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HDV1242845.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVARPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(12862..13800)
                     /gene="tdcA"
                     /locus_tag="RHV13_003340"
     CDS             complement(12862..13800)
                     /gene="tdcA"
                     /locus_tag="RHV13_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HDV1242846.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         3611 a         3764 c         3260 g         3254 t
ORIGIN      
        1 tttcccgcag acaccaggat ttcgcttccc ccatactgtc acgacgccag gccataataa
       61 tatcgatctc gctggtcgat tccgggctga cgacacgcaa ccgcccttcc gcaatatctt
      121 tttcgaccat cggatacggc atcgtcgcca cgccaagccc cgccagtaat gcctgacgtt
      181 tatcttcaat cgtgctcacc gttaagcgcg gctgtttgtc cagcagctgt acggtcaaca
      241 ccgggcgctc acgagcggta tccgccaccg caattccacg atatttcaca cgcgtcactt
      301 cagataacgg ttccggctcc tgatgaatcg ggtgatcagg cgcggcaacg tagacgttca
      361 ttaacgtata gagcttgcgc gagttgatct ccgacgagga acgaaaatgc atatccggcg
      421 cgataacaat atccgcccgc ccctgctcca gccgttccca cgcccccgcc agcacttctg
      481 tgatgattgc cagttgggta ttggcttttg ccgccagttt gtcgattaac gggaaaaagg
      541 caggtgtcgg taccagcgct tcggtcacaa tggtgagatg cgtttcccaa ccgcgcgcga
      601 gagcttccgc atcagtagtc agtttatctg cggcttccag caaaacgcgc cctcgctcca
      661 acaacatcct cccgacattg gtgaatttgg tgcgatggcc cgagcggtca aacagcacca
      721 catccagctc ttcttccagt ttttgcatgg tgtagctaag tgcggaaggc acgcgtccca
      781 gctcatccgc tgccgccgca aaactgcccc ggcgatcgat cgcatccata acccgtagtg
      841 cttccagcgt taatgccctt tctttggcca tttcgttctc attcaggaaa tttgaacata
      901 ccgggcagaa tatctggcta acaatgtagc gtccagcccc ttaccataaa aggaagtaaa
      961 gagaggtcaa gaattatgat tactacccga actgccaggc agtgtggaca agcagactac
     1021 ggatggttgc aggcccggta tactttttcc tttggacact acttcgaccc gaaattgtta
     1081 ggctatgcct ccctgcgtgt gcttaaccag gaagtgctgg ccccaggtgc cgcctttcag
     1141 ccgcgaacct atcccaaagt cgatatttta aatgtgattc tggatggaga agcagagtat
     1201 cgcgacagcg aaggcaatca tgttcaggcc agcgccggtg aggcgttgct gctctctacc
     1261 cagccgggtg tcagctacag cgaacacaat ctcagcaaag acaaaccgtt aacacgaatg
     1321 cagctttggc tggacgcctg cccgcagcga gagaatccgc tgattcaaaa actggcgctt
     1381 aatatgggca agcagcaatt aatcgcctca ccagagggtg cgatgggaag cctgcaatta
     1441 cgccagcaag tgtggctgca ccatatcgtg ctcgacaaag gcgaaagtgc gaatttccag
     1501 ttgcatgggc cacgcgcgta tttgcaatcg attcacggga aatttcatgc gcttacgcat
     1561 catgaagaga aagcggcgct gacctgcggt gatggggcgt ttattcgtga cgaggctaac
     1621 attacgctgg ttgccgattc cccactgcgc gctttgctga tagatttgcc tgtctagttg
     1681 tttttacagg gagatgatga tgagtaaaaa atcggccaaa aagcgccagc cagtgaagcc
     1741 cgtggtggcg aaagaacctg ctcgcaccgc caaaaatttt ggctatgaag agatgttgag
     1801 cgagctggaa gccatcgtcg cggatgctga aacgcgttta gccgaggatg aagctaccgc
     1861 gtaaagacag gttgccggat gcggcgaaaa cgccttatcc ggcctacaaa acacaacaac
     1921 atcaacaaat taaacacccc gtaggccgga taagatgcgc cagcatcgca tccggcatca
     1981 ggtacaaaac acaacaacat caacaagttg agcaccccgt aggccggata aggtgcgcca
     2041 gcatcgcatc cggcacgata cccaaaacct ggcgtttatc tggccttgct cgccataatc
     2101 tcgataatct gccgatccgt ttgctgcatc gaatggcttg ctaacgcaca caggttggca
     2161 atcgactgct caacatcatg cgccacgatc ccttcatcgc cggtcacggc ggtatcatcc
     2221 agcgccatta acaccgcttt ccacgcagcc gaagcactgg tcgaaacctt catcgcgcag
     2281 ctgttcgacg caccatcgca aatcatgccg ctgacatcgc cgatcatact gctgatcgcc
     2341 atcgagatgg tttcataacg cccatccacc agccatgcca tcccggcggc ggcccccatt
     2401 gctgcggtcg ttgcggcaca cagcgcagac aaacgcggta actggttatg gatgtaaatc
     2461 gcgctcaaat gcgaaagcat cagcgcacgc gccagccgtt catcatccgc tccgaagtgt
     2521 tctgctacca ccaccacagg cattgttgcg gtgatcccct ggttacccga gccggagtta
     2581 ctcatagccg gaagcgtagc gccgcccata cgcgcatcgg atgccgcgct ggtacgaatc
     2641 acaatggatg aagagagatc tttcgccagc aaaccgcgcg cgcactgttt ttccagcgtc
     2701 gcgccaatat gcagccccca cttaccgctc aaaccttcct gcgataacgc acaatttagc
     2761 ttcgcagaat cgagaataaa gcggatcgcc gcaaacggga cttcattgac gaacttcagg
     2821 atctcagcca gtgtcgttct ggaaagcacc gtcagcggag actcttgctc gccctctgcc
     2881 acacacgcct gctgggtaaa caccacacca tcgtgcgtct caatatgcac aatgttggta
     2941 tgcccgccga cgatggtgac acacgcccac ttctcaccgt tccagacttt ggcgcgtgag
     3001 aagaggattt cattgcaagg ttcctggatc ttaacggaga ctttccccgc cgccagcagt
     3061 gctttggcat cggcaattgc ctgcgcagtt gcgtctttca gcacttccag cccggcgttg
     3121 gcatttccac ctaacgcccc cagcgccgcc gcaatcggca gccccaccat tcccgtaccg
     3181 ggaacggtga cgcccagacc gttcttcatc agatttggcg aaacccaggc ttctacacgt
     3241 tcaaccggac cttccagttc tgccgcagca accgccgccg ccagcgccag tgaaatcggt
     3301 tcagtacatc ccagcgccgg ttttacttcc tcctgaactg caaggatgta acgctgccat
     3361 aacggattta aagtcgaatc aaacatatta aaaaccttaa aatttcaggt aaatcaagaa
     3421 aatgccagga acggagaaac acaaagcaac aaaccagtga cgataatcag gtacagagac
     3481 atccctttgt atttgtgcaa tgccggtact ttgtaaacca gccacgccgg gatcaggcac
     3541 cctaccatgc cgaaaatcgg gctacagata gaggtgaagc tcaacaccgg tgcgttcagt
     3601 acgatggcgc tccaggccag caaaatggcg aaaatcatga tgccgcgctg aacaagattt
     3661 tcgttaatct tctcggcagg catcttgcga cgcaggatgt tcattacgat cccttgcgtt
     3721 gcttcgcgaa agcctaaata gacgccaaag aatgcagtca tgacagcaaa gatattgaga
     3781 atgacgctga ccactttcac ccacgctgcg ccgtcaccgc taataaactg tgcggcaatc
     3841 gccagcgcgg aaatattctg ctcgtaggct tttaccgctt cgtcatgtcc catcgccagc
     3901 gtgaacgaca cggcgtagaa aaagacggtg acaaacaaaa tgccaaacgc gatattcatt
     3961 gcccgcaatg ctttatgccg cgctacttca atcgattttt cccgcgagcg ataagagatc
     4021 accatcgggc ttaacgtctg gataaacaga atcgacgtca gggtaaacgg cagcgtaata
     4081 atggcatttt tcaccagcag ccccagtggc ggtagcgaac cgacgttata cagatgccac
     4141 attcccacca tcgacacgcc cagcgcagcg accaccagca gcttggtcag caccatgccg
     4201 gtcgaaattt tgaataacaa tttctcgccg cgtgaagaga tcgccaccag aatacaaatc
     4261 agtaccagac cataaaaggg actgtctgac agcaaccctt ccgtcacgcc gaaggtatgc
     4321 agataggaag cactatcgtt ggtgatggcg gtggaataga cgaacatcca aatcaccagc
     4381 attacgaaat agagcgcacc taacaggatg ccccagtttt tacctaaata accgctaatg
     4441 acactcgggt aatctttaca ttctggtgat tctgccagcg tattaataaa caaccgctga
     4501 aacagataca ttgccgggta accaatcacc gatgagagca aaaataccca caatcccatc
     4561 aaaccgacct gcaccgggag aaaaacaatc cccgcgccaa tcgccatccc gatactcata
     4621 atcacccagc cggtgtcagt actgtcgaat ttgatcgctt ctcgccactc gctctcactc
     4681 attccagcac gacccgccgg ggtcgaagcg tctgcaataa cgcctttatt cgatgtaatt
     4741 tccataattt ctcgctcaat attttgtagg gcttattatt tttttccgag ccgcatcaag
     4801 gcgatatgcg gtctacgttt ttgcaggcga gttattagaa taaagaaatg atacgcgcga
     4861 ggtcagagaa aatcttcaca atcaagacgt tgaaataacg gagatggata agaattttct
     4921 actaaattaa tcgcaagaga atattcattc tctatttgcg gcagatcaca aaaaaaaggt
     4981 gcacatttgt gcacccaagg atgaaagctg acagcaatgt cagccgcaga ccactttaat
     5041 ggccagtcct ccgcgtgatg tttcgcggta tttatcgttc atatctttgc cggtttcata
     5101 catcgtctcg atcactttat cgagtgaaac acgcggtgcc gaggtgcggc gcatcgccat
     5161 ccgcgcggcg tttactgctt tcacggcatt aatggcatta cgttcaatgc acgggatttg
     5221 tacctgtccg gcaaccggat cgcaggtcag cccaaggtta tgctccatcg cgatttccgc
     5281 cgcattgcat acctgcgccg gactgccgcc cagtagttca gttaaccctg ccgccgccat
     5341 tgaacaggcc acgccaatct ccccctgaca gccgacttcc gcgccagaga tggaggcgtt
     5401 cattttatac agcgcgccaa tagcccccgc tgccagaaaa tagcgggcaa ttgaccgctc
     5461 gtttaccgga cgacggaact tatcgtaata agccagaact gccggaataa tgccgcacgc
     5521 accgttagtc ggtgccgtta ccacgcgccc gccagctgcg ttttcttcac taaccgccag
     5581 cgcgtacatg ttgatccagt cgatgacatt catcggatcg ttagagatgt tatcgctgga
     5641 aaccagttga cgacgcagcg ctacggcacg acgcggcaca ttgagcggac caggcagcac
     5701 gccttcagtg ttcatcccgc gttcaatacc gtcatgcatc acttgccaga tacggacaaa
     5761 actggcgtca atttccgctt tgctgcgcag cgctagctcg ttgtgcatca tcaggccaga
     5821 aatagacagg ccgttgtaat cacacatttt cagcagttca cctgctgagt ggaaatcgta
     5881 aggtacggac gtttcgacat cgtgcgacag gccgaagtgt tcttcttcga caataaaccc
     5941 gccgccgaca gagtaatagg ttttacttaa tagcgcttcc tgtcccttcc aggcagtgat
     6001 ccgcattccg ttctcatggc gaggcaacat ttcgggatgg aagataatgt tctttgctac
     6061 aggaaaatca acaatatgcg caccagatgc cactggcagc cgcccgctgc gcgttactaa
     6121 ctctataaat gcagggatct catcaatgac aacatcctgc ggactgtttc ctgccagtcc
     6181 catgatgatg gcgacatccg tggcatggcc tttgcccgtc agtgacaacg acccgtacag
     6241 atcgaccaca atatggctcg tcgcggttaa taagccgcta ctttccagcc gatcaataaa
     6301 actttttccg gcattcattg gccccacggt atgcgaactg gagggaccaa tcccaatttt
     6361 gaaaatatcg aatgcactaa tcatatccac accctcggat tgccgttcag tgaagtggag
     6421 cggaacgacc ttacgaccgt cccgctcacg aggctttacg cactacgtac tgcgatagct
     6481 tcaatttcca gcttcacatc tttcggcaaa cgcgcgacct gtacacagct ccgggtcggg
     6541 taggtcgcct gatgctcatc gaagaattgc ttatagactt cgttgatggt ggcaaaatca
     6601 ttcagatcgg tgatgaacac ggtcatcttg atgatatcgc ccacgctcag cccggcagca
     6661 accacgatcg ctttgacgtt ttcgaggctt aaacgcgcct gatcttgcac atcagacggg
     6721 atctcaccgg tctgtgggca aaccggaatt tgcccggagg tgaagaccat gctgcctaaa
     6781 tcaacgccct gaacataagg gccgattgcg cctggggcac gttgcgtttc gataatcttt
     6841 ttcatacatc ctccggcatc agagcgcctg ggtaaaggta cgtgaaataa catcctgctg
     6901 ctgttcacgg gtcagtgcgt tgaagcgcac ggcgtagcca gagacacgga ttgtcaggtt
     6961 aggatatttt tccgggtgct cgatggcatc cagcagcatt tcccgattca ttacgttgac
     7021 gttgaggtgt tgaccgcctt cgacatccgc ttcgtggtgg aaatacccat ccagcaggcc
     7081 gacaaggttg gttttacgta ctggatcttc tttgcccagc gccgcaggga cgattgagaa
     7141 ggtgtacgag atcccatctt tggcgtaggt gaacggcagt ttcgccaccg acgtcaatga
     7201 ggccacggca cctttgcggt cacgaccatg catcgggtta gcgcccggcg cgaacggtgt
     7261 tccggcgcga cgaccgtccg gcgtattacc ggttttctgg ccgtacacca cgttagaagt
     7321 gatagtcaga atcgactggg tagggacggc gttgcgatag gttggcagcg ctttaatttt
     7381 cttcataaag cgttcaacca ggtcgcaggc aatactgtct acgcgctcgt cgttgttgcc
     7441 atactgcgga tattcgccgt cgatttcaaa gtccaccgcc aggccgtttt catcacggat
     7501 tggtttcacg cgggcatatt tgatcgcaga cagggagtcc gtcgccaccg acaggcccgc
     7561 gatgccgcat gccatagtgc gatagacatc acgatcgtgc agcgccatca gcgaagcttc
     7621 gtagctgtac ttgtcgtgca tgtagtgaat gatattcagc gcgctgatgt actgcaccgc
     7681 cagccagtcc atgaagtgat cgaggctgtc catcactttg tcgtaatcca gcacgtcgtc
     7741 catcagcggt gctgttttcg gcccgacctg aatcttcagc ttctcgtcca ccccgccgtt
     7801 aattgcgtag agcagcgttt tcgccaggtt agcgcgtgca ccaaagaact gcatttgctt
     7861 accaatcacc attgggctga cgcagcaggc aatcgcgtaa tcgtcgctgt tgaagtcagt
     7921 acgcatcaga tcgtcatttt catactgcaa ggaagaggtg acgatcgaca cctgcgcggc
     7981 atattttttg aaggcaatcg gtaattcttc cgaccaaaga atggtcaggt taggttccgg
     8041 tgccggcccc atagtgtgca gggtgtgcaa atagcggaag gagtttttgg tcaccagcgt
     8101 acgaccgtcc agccccatcc cgccgatcac ttccgtcgcc cagattgggt cgccggagaa
     8161 cagcgaatca aattccggtg tacgcaggaa gcgtaccata cggatcttca tgatgaagtg
     8221 atcgatcagt tcctgtgcct gctgctcatt gaggacgcca gctttaaagt cgcgctcaat
     8281 gtagatatcg aggaacgatg ccgtgcgccc cagcgacatc gcgccgccat tttgcgattt
     8341 cactgccgcc agataagcga agtagagcca ctgcaccgct tcctgcgcat tctgcgccgg
     8401 gcgagagata tcaaagccat atttcgccgc catttcctga atctgcaaca gcgcatgacg
     8461 atgctctgcc agctcctcac gcagacgtat ggtggcttcc agatcctcgc ctttttccag
     8521 acgagactgg agatcggcaa attgcagttc gcgttcacgc accagataac gaatgccata
     8581 cagcgctacg cggcgatagt caccgataat gcgcccacgt ccataaccat ctggtaaacc
     8641 ggtcagtacg ccagatttac ggcagcgcag catatccggt gagtaaacat caaatacgcc
     8701 ctggttatgg gttttacgca gatcggtaaa cagatattca aattcactgt ccatttcacg
     8761 gccataggcg tggaatgaac ttttaatcat attaatgcca ccgaacgggt gtagcgcacg
     8821 tttcaacggc gcatccgttt gcaggccaac aattttttcc agcggctggt taatatatcc
     8881 cgcatcatga gcggtaattg tggtggcaat attggtatcg aaatcaaccg gcgcgtgggt
     8941 tgcgttttca atacgaatgc cttccattac tttttcccac aattccgtgg tggctggcgt
     9001 cgcttcagcg aggaaagatt catcgccttc atacggtgta tagttatgtt gaataaaatc
     9061 gcggacatta atttcgtttt tccagtccgt acctttaaag ccaagccatg cgtcggcgta
     9121 cagcttatcg ctggtatcaa tatctacctt catgaaaaat aatctctcta caatacttca
     9181 actaaattat gcaaattctg cgggcgcgtt aactttgcct aaatgaatgg catccaaagc
     9241 aatcattttt tcttcgttag tcggaataac ggcgcaaatg acacgcgcat tttcactgga
     9301 aacaattcgc tcgccaaaag agttagagcg attattcatt tctgtatcaa tcactacgcc
     9361 taatacagcc aaatgttcca tgaccagacg acgaattaaa cttgaattct ctcctattcc
     9421 gccggtgaat ataattccat ccaggcgacg taatgaagct gcgtgtccgg caatatgacg
     9481 ggcaattcgg tgaacaaagg ttttaattgc cagttgcgcg cgttcgtgac cttcatgcca
     9541 ggctttttcc agaacacgta aatccgaaga aagaccggaa atacctaata atcccgactc
     9601 tttattcact acgcgttcca ggtcacccag gctctggttg gtttggctgg cgacccagga
     9661 catcgcacca aagtcgacat cgccactgcg ggtacccatc atcaagcctt ccagcggcgt
     9721 cattcccatt gaggtgtcaa cactctgacc gttgcgaact gcgcagattg acgcgccatt
     9781 gccaagatgc gccacaacca ggccggagtc atcttccgcc agattcagca gcgaatgtgc
     9841 gcgctgggaa acatagcggt gcgacgtgcc gtggaaacca taacggcgta cacctaactc
     9901 ttcataatat ttccacggca ggccgtataa ataagcttcc ggagccatcg tctggtggaa
     9961 actggtatca aataccgcca cctgagttac gcccggaaat aattgctgcg ccgattcaat
    10021 accacttaaa ttggcgtaat tatgcagggg tgccagtgga gaaacgcgac ggatattatc
    10081 aatgacttca tcggtaataa tggcggactc ggtaaaaata ctgccgccgt gagcgatgcg
    10141 gtggccaatt aaggccacac tgtcatttaa attccgtttt tccagttcaa atgcaattgc
    10201 cttcaatgca ccttcgtagc tgtggtgagc cagcggtgct ggctctcccc catttacgga
    10261 taagaatgca ttttccgagt taataccgtc ggcaatacct gacattaata cttcacagtc
    10321 gctggcatcg agtacggaaa acttaatcga agacgaacca cagttaataa ccaaaacaac
    10381 cggaaattca ttcatctctt ttctcatcct gagttacgga ttaaaacagt ttgtatacga
    10441 tgttcaggat ggtcagcaga ccaatcacgg taacaaacac gttatccaga cgaccacggt
    10501 atttcgccag agacggcgct ttacggatgg catacatcgg caacaggcac agcagggatg
    10561 cgataatcgg tgcgcccatg gcttcaatca ggtcgaggat gttcgggttg gcgtaggcaa
    10621 caacccaggt ggagcccatg atgaagatca tgctgagagt attcagttta cccagcgaca
    10681 ctttggtttt gtcaccttta taaccgaact tcagaatcag accattcaag ccttccagcg
    10741 tccccagata gtgaccgaag aaagatttga agatagccac gagtgcgatg atggaagccg
    10801 catattccag tgtaattgcg aacgttgttt tcgtaccggt catggacgca aagtggttag
    10861 ccagataaga aagcactgga atattctgcg ctttggcttc cgccatgttg gccggagaca
    10921 gcgtaaacag gcagctaaag gcaaagaaca tcaccactgc aaccatcagc atgctggcac
    10981 gagaaatgat ttgggaacat ttacgttcgg tgaagtcgcg accgaagtct ttctcatact
    11041 cttcacgttt agaaaccacg aaggaagaga cgattggcga gaagttaaag gagaaaacca
    11101 tgatggaaat ccccagccag acagtgatca ggataccgtc atgaccggtt aacgacagcg
    11161 aaccgaggtc aacctggtcg ataaccgcag agttccagta agggatcagc gacagagaaa
    11221 tcagcaccag gctggcgata aacggccata ccaggtagct catcacttta accatcagat
    11281 ccttaccaaa ccagatgacg aaagccatca gcagcagcag gaacagcgcc acaaagccgc
    11341 gattcagcgg tgcaaagccg agctggtttt cccagaacgt cataaaggta ttggtaatag
    11401 taacgccata aatccacagc agtgggcaaa tcgcgaagaa gtacaggaac gtgataacca
    11461 cgccgccagt tttaccaaaa tgctcttcca ccgtttccgt aatgttgccg gaagggttag
    11521 agccagaaag acacagacgc gccagcgccc ggtggcaata aaatgcgatg gggtatgcca
    11581 ataccaacat cagaagaatc gggatcagtc cgccaaaacc tgcgcggata gggaagaaca
    11641 gcaccccggc gccgattgcc gtaccaaaca agcctaacgt ccatgtggta tctgatttac
    11701 gccaggacga ttgttttgtc tggctggata caatgctatc tgaagtactc atatcctatc
    11761 ctcaacgaat taattaagcg tcaacgaaac cggtgatttg agagacgcga gaaagatcga
    11821 tattgccgcc ggaaataata ctgacggttt ttctgttttg aatatattgg tctaatttac
    11881 cgcttaataa tgcagcacat gccagagcgc ctgcgccttc ggtgacgact ttattgcgct
    11941 gaattaaggc aatcatactg tttctgattt cgtcttcgct gaccagcacg atgtcatcga
    12001 ctaattcacg aacgatttcg taagttaaat tacccgggcg ggcgacatca caaccatccg
    12061 ccagggtgcc ggtagttcgg tgcgtggtta tttctccgga gtggaaagaa gccgccatgc
    12121 cgtgaacgtt ttcagactgt acgccaataa cacgaatggt cgggttaata gatttaattg
    12181 ccaccgcaat accagcaatt aaaccgccac caccaattgg cacaatcacg ttatcgacat
    12241 catagagatc ttccataatt tccagaccaa tcgttccctg gccagcaatc actttcggat
    12301 catcgtaagg tgggataaaa atacggcctt ccatttcgac aatttcgctc actttagcga
    12361 tagtgtcgtt gaagttatca ccatgcagaa cgacttctgc ggagtagtcg cacgttgccg
    12421 ctactttgga ttttggcgca ccttttggca tcaccacttt accgtcgata cccagcatcg
    12481 cgcaggagag ggaaacccct tgcgcatggt tgcccgcaga acaggccacc acgcctttgc
    12541 gtttttccgc atcggtcagt gaacttaatt tattaaatgc gccacgaatt ttaaatgaac
    12601 ccgtacgctg catattttca aacttcagga atatttcacc tttgcaacgt tcactaaaat
    12661 agttggagcg aggcatgcct gttttataaa ttcgcccagc cagtcgttgt ttcgcttcaa
    12721 taatgtcatc aatagcaacc ggcagatcgt atgtaatatg cattataaaa cctcttcgcc
    12781 tgaattaaat atgtaggtaa ccgtaaccga cacctgcaaa acagacaggt ggattattta
    12841 tgtttaaata aaaacaaata actaaccaac ttctattaat tgccttcgtc tacacccatt
    12901 ataagatgaa tactctttgg ctaattccac caaaaccgat gctgcttttt taatacgata
    12961 atttttcgac cataccgcgg catattgtgc cacaggtaat gtttcttcaa ccggaatagt
    13021 aataaattga ttagaaccaa aaggtgacgt catatcacaa ggaattacag ttaagaaatc
    13081 agcattgaga acaagattat aaattgtcac gactgagtcg gttttaacga tgttttcaat
    13141 actgatgcca tttctttgta acgtagtaag cagttcgctg tagtacccca tattagtttg
    13201 tggcaacacc cactgttcgt tcttcaacga ctccagcgtg gtggtgccgg tgcatgttcg
    13261 ggacttactg gctaccagca caaactcgga ctcgaacagc ggctcaacat gtaaatcctg
    13321 aagcttcatt tctgcactta acgtaccaat cgcaaaatca agtcgaccgt cgcggattgc
    13381 cggtaagaac gaagacagtt gcgcttcata catagaaacc tgcgctttcg ggaacacctc
    13441 tttgaacttg ttgatcatcc ctgacataaa agtaaaacca atcaatgaag gaaaaccaaa
    13501 tgagacttcc accaccgcct cagaagacat accgcttatc tcattaacca tatttttcat
    13561 ttcacgggta atggattcgg aacgagagag taacaattga ccggcaggtg ttaatgttac
    13621 accggtattt ttccgcacca ctaattccac accaaaataa tcttcaatat cgttaatgat
    13681 tttactgacg gctggttgag ttaaccctaa ttcttttgca gccgagccga tagagccact
    13741 tctaatgact tcctgaaaga ctaccaggtg ctgcgttttc ggaagaagaa tagtgctcat
    13801 aacgacctac gttaattacc tcattgacgg catgaagtgt atcaaaatga aatgaacagg
    13861 atatgtgcga ccactcacaa attaacttt
//
LOCUS       DAPGOF010000126        13812 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.127, whole genome shotgun sequence.
ACCESSION   DAPGOF010000126 DAPGOF010000000
VERSION     DAPGOF010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.127"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(262..777)
                     /gene="pgpA"
                     /locus_tag="RHV13_003341"
     CDS             complement(262..777)
                     /gene="pgpA"
                     /locus_tag="RHV13_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HDV1242847.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGIL"
     gene            complement(755..1732)
                     /gene="thiL"
                     /locus_tag="RHV13_003342"
     CDS             complement(755..1732)
                     /gene="thiL"
                     /locus_tag="RHV13_003342"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HDV1242848.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            complement(1810..2229)
                     /gene="nusB"
                     /locus_tag="RHV13_003343"
     CDS             complement(1810..2229)
                     /gene="nusB"
                     /locus_tag="RHV13_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDV1242849.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(2249..2719)
                     /gene="ribE"
                     /locus_tag="RHV13_003344"
     CDS             complement(2249..2719)
                     /gene="ribE"
                     /locus_tag="RHV13_003344"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HDV1242850.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(2808..3911)
                     /gene="ribD"
                     /locus_tag="RHV13_003345"
     CDS             complement(2808..3911)
                     /gene="ribD"
                     /locus_tag="RHV13_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HDV1242851.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPIRIVIDSQNRVTPVHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(3915..4364)
                     /gene="nrdR"
                     /locus_tag="RHV13_003346"
     CDS             complement(3915..4364)
                     /gene="nrdR"
                     /locus_tag="RHV13_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HDV1242852.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            4515..5054
                     /locus_tag="RHV13_003347"
     CDS             4515..5054
                     /locus_tag="RHV13_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HDV1242853.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            5353..6237
                     /gene="tsx"
                     /locus_tag="RHV13_003348"
     CDS             5353..6237
                     /gene="tsx"
                     /locus_tag="RHV13_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HDV1242854.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(6275..6622)
                     /locus_tag="RHV13_003349"
     CDS             complement(6275..6622)
                     /locus_tag="RHV13_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HDV1242855.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(6751..7722)
                     /gene="secF"
                     /locus_tag="RHV13_003350"
     CDS             complement(6751..7722)
                     /gene="secF"
                     /locus_tag="RHV13_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HDV1242856.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(7733..9580)
                     /gene="secD"
                     /locus_tag="RHV13_003351"
     CDS             complement(7733..9580)
                     /gene="secD"
                     /locus_tag="RHV13_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HDV1242857.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(9608..9940)
                     /gene="yajC"
                     /locus_tag="RHV13_003352"
     CDS             complement(9608..9940)
                     /gene="yajC"
                     /locus_tag="RHV13_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDV1242858.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(9963..11090)
                     /gene="tgt"
                     /locus_tag="RHV13_003353"
     CDS             complement(9963..11090)
                     /gene="tgt"
                     /locus_tag="RHV13_003353"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDV1242859.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(11145..12215)
                     /gene="queA"
                     /locus_tag="RHV13_003354"
     CDS             complement(11145..12215)
                     /gene="queA"
                     /locus_tag="RHV13_003354"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDV1242860.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            12308..12889
                     /gene="acpH"
                     /locus_tag="RHV13_003355"
     CDS             12308..12889
                     /gene="acpH"
                     /locus_tag="RHV13_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HDV1242861.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVITILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLNAHYAALETRFWQFYPRMMAQASRKAL"
     gene            complement(12910..>13812)
                     /locus_tag="RHV13_003356"
     CDS             complement(12910..>13812)
                     /locus_tag="RHV13_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242862.1"
                     /translation="RTKQLEKKITDAGFITGKVNAEKFNVKEFKDDITSMLCEQPPLR
                     THQISSSGYVSADKLPKLYELNLKDAQRDLKTTPVDIDGVSLKFKTRERIAEEIAKKT
                     AEKTAKKNSEIIAKQNANSIEEKNIEKNNKETNPEDNFVLKFSQEEINEMLLDYSDEE
                     QKKLRPFFTSDHIYCLFDAFSQCLYNVARDGSPRYAGLLQQIASKCAILQDFDQQLFW
                     RTFSGGGFHRRISVNSEPEKTDVWRFSTQFDFPHINDNGELSRTVLMFEGVAESNFKD
                     IANHSSECFSYRCYAAPLDANAKK"
BASE COUNT         3411 a         3691 c         3317 g         3393 t
ORIGIN      
        1 gaccgaaacc attgagaata attttgatga tatttttcgt caacagtcag agaatatgag
       61 caattattat gagttcgtcg actgacgata tttcagtgtc attgattatg agtgacaaag
      121 atacaccttg aaatatcgcc gcatagtata ttcggtgatg tcgggtttta ctttaacaga
      181 gtgccccaaa attggggcac tgttcattta taaatcaaac cgggtggtct ggaatcgcct
      241 acacctcccg gtcaatggca tttaaagaat acccagcggc cagtgatgac cgataaagta
      301 caggatgcct gcggaaatca ccccggcgac aatatcatcg atcatgatcc ccatgccgcc
      361 atgcacattg cgatcaaacc agcggatcgg ccacggtttc cacatatcca gaatacggaa
      421 aatcacaaac ccggcggcaa cccactgcca gtcattggtc ggcagcgcca tgagcgtgat
      481 ccacatacca ataaattcgt cccagacaat gctgccgtga tcgtgcacgc ccatatcttt
      541 tgcggtctga tgacaaagat agacgccgat acagatcccc agcatcacca ccagcgagta
      601 gagctgccag ggcaaaaagg tcatcagata ccagaacgga atcgctgcca gcgagcccat
      661 cgtcccagga acgatcgggc ttaatccact tccgaatccg acagcaagta gatgccacgg
      721 attactcatc ttcaggcgac ttttcgcgac atctttatgg cgtggcaaaa tggtcatatc
      781 ctttccagtc taacgtaaca ggttcgccgt cacgaataaa acaaagccct tcgatatcgg
      841 cggtcatttg cccgatacag gtaaacggta cgccaaggtg tccgagagca acatccagcg
      901 cgccacggtt cagttccggc accgtgaaac acaactcgta atcttcaccg ccagagagcg
      961 cccagcgcag cgcctgttcc ggttcaacat ggcgagaaag cgcatcagaa aacggcagca
     1021 atgccaggtc aatacgtgcg ccgcagtcgc tggctttcac gatatgcccg agatcggaaa
     1081 tcagaccgtc agagagatcg atagctgaat ttgccagatc gcgcagtgcc tgcccctgta
     1141 aaatacgcgg cgatggacgg agatgacgtt tgatcaagta gtccgcatct ttagcatcgg
     1201 caacctgcaa acggttttgc aaaatcgcca gcccggcggc gctatcgccc ggtgtaccgg
     1261 tcacatagat ccagtcaccc ggtttcgccc cagagcgcgt taaggctcgt cccatcggaa
     1321 caaagccgtg gatacccaac gtcattgata atggcccacg cgtggtatcg ccgccaatga
     1381 gttgcatatc gtaataattg agaagatcaa acaaactgtc gctgaaggac tcaagccacg
     1441 cttcgtctac gtccggtaag gttaatgcca gcgtcagcca ggccggatcg gcccccatcg
     1501 ctgccagatc gcttaggttc accgccagtg ctttataagc cagatcagca ggatcgatat
     1561 cagggaggaa gtggttgccc gccaccagcg tatcagtgct gatcgccagg gtctgtttct
     1621 cggggatatt gagaagagcg caatcgtcgc cgatgcccag ttcgacatca agacgagaac
     1681 ttcttacacg gtcaaaataa cgggcaatca gggagaactc gccacatgcc atacgttatg
     1741 cctcagcagg taaaaaagaa aaggccggcg atcgcggaac agtcttccgt gaatctaccg
     1801 gcctggatat cactttttgt tagggcgaat cacaggtgct gctttatcga gtacgccgtt
     1861 gacgaactta tggctgtctt ctgcgccgaa cgatttcgcc agttcgatcg cttcgttaat
     1921 ggccactttg tatggcacat cgctacgttt agacagttcg tacagcgcaa tgcgcagtac
     1981 tgctttttct acctgtccca gttcttccag caggcgggac aggtatggct tcatcagtcc
     2041 gtcgaggtat gcggtattag tcgccacccc ggccagcagc tcacggaagt acaggacgtc
     2101 aacgtctttt acatcctgtt cagccaggaa ctggtattca acatcagcga tgtcgttctg
     2161 ggacaactgc caggagtaga gcgcctggac ggcacactca cgagcgcggc gacgagcagc
     2221 aggtttcacg gatttcccct tactaatttc aggccttgat ggctttcaat acattaatca
     2281 tttcaagcgc ggtcagtgca gcttctgcac ctttgttgcc agctttggtg ccagcacgtt
     2341 cgatcgcttg ttcaatgctt tcagtggtca gaaccccaaa agcaaccgga atttcgctgt
     2401 cctgggcaac atgcgccagg ccgttgcttg caccaccagc gacatattca aaatgggcag
     2461 tgccaccacg aataaccgta cccagcgcaa tcaccgcgtc gtatttaccg gttttagcca
     2521 gtgcacccgc cgccagcggc agctcatagg caccaggcac ccaaacaacg gtaatgtttt
     2581 catcttttac ctgaccgata cgtttcagtg catcaattgc accttccagc aggctgtcat
     2641 tgataaagtt gttgaaacgc gcgatggtga tggcgacgcg agcgtccggg gtagcaacgt
     2701 tagcttcaat aatgttcatt ttcttccttc gggttcgagt atggccccgc aggggggcgg
     2761 attttagcat aatatttcgt gcgctgcttc cctttcgagc cgggagatca tgcacccact
     2821 aaatgcaggc aaacatccgg gcctacatga cgtatctctt tgaatttaaa ttggggggcg
     2881 tcagctaatt tctcaagccc tggcagtgtg cataatccac gggcgtcgct gcctaatagt
     2941 ttaggtgcga tatagacaat cagctcatcg actaaacccg cctgcagcaa tgcgccagcg
     3001 agcgttggcc ccgcttccac ccagatgctg ttaatctgct gtttacccag ttgcatcatc
     3061 agtacaacca gatccagatg acctttatgc tctggaatca gcaaggtacg caccgtttcc
     3121 ggccactcac gagaatcttc ctgcgtacgc gcgaaccagg tttcgccggg ctgctgcaca
     3181 atgcgatgta ccggcgtcac gcgattttgg ctatcaatca caatacgtat cggctgacgg
     3241 agattttgtt gcggatagag cgcctgagtt tgttcatcca gttcagacca acgcaccgtt
     3301 aaggcaggat catccgccag caccgtagcg ctgctggtta aaatggcatg actttgtgcg
     3361 cgcagtcgtt gtacgtcgcg ccgcgcctga ggcgaagtga tccactggct ttcgccgctc
     3421 gccatcgccg tgcggccatc aagcgatgcg ccaagtttta actgaatata aggaaagccg
     3481 gtgcgcatcc gcttgagaaa gcctttattc aattgctcgg cttcactcat catcagcccg
     3541 tggctgacgt caatgccagc ctgttgcaaa cggtaaagtc cacgcccagc gacctgcggg
     3601 ttcgggtcct gcatcgcagc gaccacgcgc gctacgccag cggcgatgag tgcgtcacag
     3661 cacggtggcg tacgaccatg atggctacag ggttcgagtg tgacataggc ggtcgcacct
     3721 ttggcttttt cacccgccat acgcaacgcg tgtacttcgg catgtggttc acccgctcgt
     3781 tggtggtaac cttcaccgac aatttcgcca tctttgacga tgacgcaccc gacattcggg
     3841 ttggggtggg tggtaaaacg tcctcgttgc gccagcttta gcgcccgcgc catgtaatac
     3901 tcgtcctgca cggcttagtc ctccaggcgc gcgatctctt cgccaaattc tttgatatct
     3961 tcgaaactgc ggtagacaga ggcaaaacgg atataggcga ctttatcgag ctttttcaat
     4021 tgctccatca ccagattgcc aatcatcttg ctcggcactt cgcgctcacc ggtggcgcgc
     4081 agttgcgatt taatatgatt gattgccatt tcgacgtcat cggaactcac cggacgtttt
     4141 tccagcgccc gcagcattcc gctacgtaat ttttcttcat taaacggttc acgcacgtcg
     4201 ttgcttttta caacacgcgg cataaccagc tccgccactt caaaggtggt gaaacgttca
     4261 ttacacacca gacactgccg acggcggcgt acggatgaac cctcgcccac gagacgagag
     4321 tcaattacct tagtgtccac ggcgaaacag aatgggcaat gcatacggcg tcctgaccag
     4381 gtggttaaca gaaatctatt ttaccctgaa ctgacgatac aacaaaggcg caacgctttt
     4441 gagataaagg atcgatgctt tcgctgcttt taccctctac cattaggcta attgcgacga
     4501 gaaagggacg acctatgaat actaacgttt ttcgactgct cctgctggga agcctgttca
     4561 gccttagcgc ctgtgtgcag caaagcgaag tgcgacagat gaaacacagc gtcagcacgc
     4621 tgaaccagga gatgacgcaa ctcaataaag aaaccgtcaa aatcactcag caaaacaggc
     4681 tgaatgcaaa atccagcagt ggggtttacc ttctgcctgg agcgaaaaca ccggcaagac
     4741 tggaaagcca gatcggtact ttacgtatgt cgctggtgaa tattacgcct gatacagatg
     4801 gcaccaccct gacactacgt attcagggtg agtcgaatga tcccttgccc gcattcagcg
     4861 gaactgttga atatgggcaa attcagggaa caatagacaa ctttcaggaa atcaatgtac
     4921 agaatcaatt gattaatgcc cctgccagcg tcctcgcccc cagcgatgtt gatattccgt
     4981 tacagttaaa ggggatttct gtggatcagt taggctttgt acgtatccac gacattcaac
     5041 ctgttatgca gtaaacgtat agcggggcga ttttgtgtgt cccgcaacat ccttccccat
     5101 cattttgtta ctccgcttac ataacccaga ttgatagtaa aagtttgcaa caagggcgaa
     5161 agtcagtaca atccccgccc gaatgtgtgt aaacgtgaac gcaatcgatt acgtaaatga
     5221 tagaactgtg aaacgaaaca tatttttgtg agcaatgatt tttataatag gctcctctgt
     5281 atacgaaata tttagaaacg caatttgcgc ctttttcact cccgcaaggg attttcaaac
     5341 agtggcatac atatgaaaaa aacattactg gcagccggtg cggtactggc gctctcttcg
     5401 tcttttactg tcaacgcagc tgaaaacgac aaaccgcagt atctttccga ctggtggcac
     5461 cagagcgtta acgttgtcgg aagctatcac acccgtttcg gaccgcagat ccgcaacgat
     5521 acctaccttg agtacgaagc attcgctaaa aaagactggt tcgacttcta tggttatgcg
     5581 gatgcgccgg tattcttcgg cggtaactcc gatgcaaaag gtatctggaa ccacggttct
     5641 ccgctgttta tggaaatcga accacgtttc tccatcgaca agctgaccaa tactgacctt
     5701 agcttcggtc cgttcaaaga gtggtacttc gcgaacaact acatttacga catgggtcgt
     5761 aataaagatg gtcgccagag cacctggtac atgggtctgg gtaccgacat cgatacaggt
     5821 ctgccgatga gcctgtccat gaacgtctat gcgaaatacc agtggcagaa ctatggcgca
     5881 gcgaacgaaa acgagtggga cggttaccgt ttcaaaatta aatactttgt gccgattacc
     5941 gatctgtggg gcggtcagct gagctacatc ggcttcacca acttcgactg gggttccgat
     6001 ttaggggatg acagcggtaa cgcaatcaac ggtattaaga cccgtactaa taactctatc
     6061 gcttccagcc atattctggc tctgaactac gatcactggc actactctgt cgtagctcgt
     6121 tactggcacg acggtggtca gtggaacgac gatgcagaac tgaacttcgg caacggtaac
     6181 ttcaacgttc gctctaccgg ctggggtggt tacctggtag taggttacaa cttctaattg
     6241 tgagaatgcc ggatttcata tccggcattt tttatcactt cttcttgttc atcatcgctt
     6301 tcaggtcagc gaatgggttg tacttcgctt caccgacatc tttctgcgca tcttcccctg
     6361 cgataacggt cgtaccatac tgatccgctt cggtatattt cgaatgctca tgatcgtggc
     6421 aatagagaca aagcaattcc cagttactgc catcttccgg gttattggta tggtcgtgat
     6481 caatgtggtg aacagtaagt tcacgcaggt tggaatagac aaactcgcgg gaacagcgac
     6541 cgcagaccca cggatagatt tttaatgctt tttcgcgata gccgctttct aaccgcgcat
     6601 agttttttgg gatgatagcc atgaaaagtt acctggatga aatgtagggt tacgcgcagt
     6661 ttcccaagat gcggggattt tcgcaatctc catacatcaa aaaaaatccc gatcttctga
     6721 ccgggatttt caacatcaac gggaacttga ttaaggcaga attgacggct gatccgcccc
     6781 ttctttttcc actttctgct gcaacatgtg ttcgcgcttc atacccagtt tcagagccaa
     6841 cgcggatgcc acatagatgg aagatgcagt accgatggaa acaccaatca gcatggtcag
     6901 cgagaagcct tccagtaccg gaccaccgaa gaggtacagc atcaggataa ccatcaaggt
     6961 agtaccggat gtgatcaagg tacgatgcag cgtctgggtc aaggacacgt taaagatttc
     7021 gtaaggcgta ccgcgacgaa tcttgcggaa gttttcacga atacggtccg ataccacgat
     7081 actgtcgtta agcgagtaac cgataaccga catcaacgat gccacaatgg tcaggtcaat
     7141 ctcgatatgg aataacgaca aaatacccag cgtaatgata acgtcgtgcg ccagcgcaat
     7201 aacgacccct gccgccagtc gccactcaaa gcggaaacct acgtacacga ggatagacag
     7261 cagcgctgcc atcaacgcca tcgcaccggt ttgcgcaagg tctgccccca cgctcggacc
     7321 gacgaactca atacgcttca ctgctgcatt ctgattggtg gattcgttaa tcaccttcag
     7381 aacctggctg cccagcacct gaccgccggt ttcgccttca gcaggcggca tacggaccat
     7441 gatgtcgtgg ctgctaccaa agttttgcag catcggctct tcaaaaccgg ctttttgcaa
     7501 tgcatcacgc attacgtcaa tttcagccgg tttttcgagc gtaatttcga taaccgtacc
     7561 accggtgaaa tccagccccc agttaaagcc gcgcacgccc ataataacga tagcagcgat
     7621 taacagcaga ccagagatgc caaaagccca gtagtcccag cgcataaagt catagacttt
     7681 acggccgtgg tttagttgtt caacagtata ttcctgtgcc acatcgcact cctcagattg
     7741 acagcttctt gacgcgcttg ccgccatata gcaggtttac gatggcacgc gtaccgacaa
     7801 tcgcggtaaa catcgacgtc gccacaccga taccggtagt aatcgcgaac cctttaattg
     7861 ccccggtacc cactgcgtac aggatgatga ctttaatcag cgtggtgatg ttcgcatcga
     7921 agatagaact gaatgcgcca cgataacctt catcaattgc ctgttgaaca gtacgtccgt
     7981 tgctcaactc ttctttaata cgttcgttga tcagtacgtt cgcatcgacc gctaccgcaa
     8041 gggttaagac gatacccgcg atacctggca tactcagtgt tgcgcctggc aacagcgaca
     8101 taataccgac gatcaagatc aagttggcaa tcagagcact ggtcgcaatc agaccaaact
     8161 tcttatagaa gatgatcatg aacagaatag aaaccagcag accggcaagg caagcctcca
     8221 gcccctgctc aatgttctgc atacccaggg tcgggccaat tgtacgttct tcaacaatct
     8281 gaatcggcgc gatcaacgca ccggcacgca gcagcagtga cagctgacgg gcttcgttcg
     8341 ggttgttgat gccggtgata cggaagctgt taccaagacg agactggatg ttggcgatgt
     8401 taatcacctc ttcctgtttc accagaaccg cacgaccatt tgcatctttc ttaccgctgt
     8461 ctttgtactc cacaaacagg gttgccatcg gtttgccgat gttgtccttg gtgaagttag
     8521 acataatgtt gccaccagcg ctatcgagcg agatgttaac ctgcggctgg ttgtattcgt
     8581 cctggctgga agtggagtcg gtgatatggt caccggtcag aattacgcgt ttgtacagca
     8641 caaccggctg accttcgcgg gtctgtttca cttcagagtc gcccggtacg cgaccggatg
     8701 ccgcagcggc ctgatcaacg ttggtgttta ccagacggaa ttccagcgtt gccgtcgcac
     8761 ccagaatctc tttcgcacgc gcagtgtcct gaatacctgg cagttcaaca acgatacggt
     8821 cagcaccctg acgctgaacc accggctccg ccacgccaag ttggtttaca cggttacgca
     8881 ggatattgat gttctgctgc accgcatatt cacgcgcttc actcagacga gcatcgctca
     8941 ttaccgcacg cagctggttg ctgccctgac tgctaatcac caggtccgga tgacgcttgc
     9001 tcagatacgc aatggcttca tcacgagctt tagcatcgcg gaaagtgatg ctcaggccgt
     9061 agttattttc tttacgaacg gtggtatacg ggatgccttt ttcgcgcagg tcgctgcgca
     9121 ggctatcgat attttgttcc tggagtttgc caagcgcggt gtccatatcc acttccatca
     9181 ggaagtgaac gccgccacgc aggtcaaggc cgagcttcat cggctcagcg tgaatagctg
     9241 ccagccagcg cggcgttgcc ggggcaaggt taagcgccac gacgtatttg tcacccataa
     9301 cgcccattaa tgcttcacga gcgcgcagct gggtgtcagt ggagtcgaag cgcgcaagaa
     9361 tagcgccctc ttccagtgcc acagacttag cagttatttt ttcttcttgt aacgttttct
     9421 ggacctggat cagcgtttgc tcactggcgg cgactccgcg cgcaccagtg atctgaacag
     9481 ccggatcctc accaaacagg ttgggaagcg catacagcag accgatgaca atcaccacga
     9541 tcagcatgac gtacttccac aaaggataac ggtttaacac ggcaattccc ttagggaaaa
     9601 attttaatta cagcgccttc atggtgcctt tcggcaggac ggcagctacg aagtcacgtt
     9661 taataactac ttcagtggtg tcattcagcg cgatagcaat gtagccgttt tccgctactt
     9721 tggttacgcg accaaccagg ccaccgttcg tcagaacttc atcacctttg gcaatggagt
     9781 ccatcagctt tttgtgttct ttggtgcgct tctgctgtgg acgcaggatc atgaaataga
     9841 aaatcagacc gaataccacc agcatcaaaa tcaaagacat cgggctacct tgcgccggtg
     9901 cacccgttgc cgctaccgca tcagaaataa aaaagctcat taaatttccc tcattattaa
     9961 tattaatcaa cgttcaaagg tggtacttct cgcccctgac gctggtaaaa atcagttacg
    10021 aagctctcta atttaccctc ttcaatagcc ttgcgtaaac ccgccatcaa acgctggtag
    10081 taacgaaggt tatgaatggt attgagtcgc gcgcctaata tttcgttgca acggtcaaga
    10141 tgatgcaagt aagcgcgtga ataattgcga caggtgtagc aatcacactc aggatcgagt
    10201 gggccagtat cgctcttata cttcgcattg cggattttca ccacgccatc ggtcacgaac
    10261 aaatgaccat ttcgggcgtt gcgggttggc attacgcagt caaacatatc gataccacga
    10321 cgtacgcctt caaccaggtc ttctggttta ccaacgccca tcaggtaacg cggtttgtct
    10381 gccggaattt gcgggcatac atgctccaga atgcggtgca tatctgcttt cggctcaccc
    10441 acagcgagac cgccgacagc gtagccatca aaaccgatat ctaccagacc tttaacagaa
    10501 atatcacgta aatcttcgta aacgctgccc tgaatgatac caaacagcgc atttttgttt
    10561 ccgagactgt caaaacgctc acggctacgc ttcgcccaac gcagagacat ctccatggag
    10621 cgttttgcgt aatcccagtc agcaggatac ggcgtacact catcaaagat catgacgata
    10681 tccgaaccaa gatcgtactg aatctccatc gacttttccg ggtcgaggaa aatcgggtcg
    10741 ccgttgatcg ggttacggaa gtgaacgccc tgttcggtga ttttacgaat atcgccaagg
    10801 ctgaagacct ggaagccgcc ggagtcggtg aggatcggtc ccttccactg cataaaatcg
    10861 tgcagatcgc cgtgcagttt catgatttcc tggcccgggc gcagccacag gtggaaggtg
    10921 ttgccgagga taatttgtgc gccagtggct tcaacttctt ccggcgtcat cccttttacg
    10981 gtgccgtagg tgccaacagg cataaaacaa ggcgtttcca ctacgccacg atcaaagacc
    11041 aggcggccac ggcgtgcgcg accgtcggtg gtgtccagtt caaatttcat tttttctcca
    11101 ctacgtcaga aaaacagtcc aacgtttaaa ccagcgccgt ggaattactc cccgacgcgc
    11161 tcattaattg cctgcggatt gtacgtgata aacatcgcat caccgtaact aaaaaagcga
    11221 tatttctctt ctaccgccgc tttataggca ttcatggtgt gttgataacc ggcaaaggcc
    11281 gaaaccagca taatcagcgt cgactctggc aagtggaagt ttgtcaccag cgcatcgacc
    11341 actttgtact ggaagcccgg atagataaag atttgggtat cgtcaaagaa cggttcgatg
    11401 agatcgtttt tcgccgcctg agccgcgctt tccagcgaac gtactgaggt agtgccaacc
    11461 gcaatcaccc gattaccgcg tgctttcgcc gccagtaccg cgtctaccac atcctgcggc
    11521 acttcagcgt attccgagtg catgatgtga tcttcaatgg tgtcgacgcg caccggctgg
    11581 aaggtgcccg caccaacgtg cagggtgacg aacgccatct ccacgccttt ggcgcgtaat
    11641 ttttccagca aaggttcgtc aaaatgcaga ccagcggtcg gggctgcaac cgcacccggt
    11701 ttttcgctat aaacggtttg ataaagttcg cggtcagcgt cttcgtccgg acggtcgata
    11761 tacggcggca gcggcatatg accgatgctg ttgagaatat ccagcaccga acgctcatca
    11821 ttaaattcga cttcaaacag tgcgccgtgg cgcgcggtca ttgttgcgtt aatactttcg
    11881 tcatcgccca gcagcagttc tgcgccaggt tttggcgctt tcgaggcgcg aatatgcgca
    11941 agaatgcgtt tgtcgtcgag catccgctca accagcactt caatcttgcc gccactggct
    12001 ttacgcccaa acagacgcgc cgggatcacc cgggtattat taaaaaccag aagatcgccg
    12061 gggttgagct tatcgagtaa atcggtgaaa gtaccgtgcg tcagcgcgcc cgtcggcccg
    12121 tccagcgaca gtaaacgaca gctactgcgt tcaggcatcg gatagtgggc aatcagggat
    12181 tcgggcaatt caaaggagaa atcggtaacg cgcatgacac tgactcagac taaaataaga
    12241 ggcgggtagt ctagtgccgg ggcgctctcc ctgcaacctt tacccccctt tggatagaat
    12301 ataatcgatg aattttttag ctcacctgca tttagcccat ctcgcggaaa gctcgctttc
    12361 cggcaatttg ctggctgatt tcgtacgcgg aaatcctgaa gaaagttttc cgcccgacgt
    12421 cgtggctggc attcatatgc atcgacgtat cgacgtattg actgacaatc tgccggaagt
    12481 ccgcgaagca cgggagtggt ttcgtagtga aactcgccgc gttgcgccta ttacgctgga
    12541 tgtcatgtgg gatcactttc tttcccgtca ctggtcgcag ctgtcgccgg actttccgct
    12601 acaggaattt gtttgttatg cccgagaaca agtgataacg attttgccgg actcaccgcc
    12661 acgctttatc aacctgaaca actacttatg gtcagagcag tggctggtgc gctatcgcga
    12721 tatggatttc atccagaacg tgttaaatgg catggcaagc cgtcgcccac gtctggatgc
    12781 cctgcgtgac tcctggtacg atttaaacgc ccattatgcc gccctcgaaa cccgcttctg
    12841 gcagttttat ccgcggatga tggcgcaggc gtcacgcaag gcgctctgaa acgtcaaact
    12901 ccggcgtcac tactttttag cattcgcatc caacggggcg gcataacaac ggtaagaaaa
    12961 acattcgctg gaatgattgg cgatatcttt aaagttgctt tcagcaaccc cctcgaacat
    13021 gagtaccgtt ctggacaact ctccgttgtc atttatatgt ggaaaatcaa attgtgtact
    13081 gaatcgccat acatctgtct tttctggctc gctgttcaca gagatacgtc gatggaagcc
    13141 tcctcctgaa aacgtgcgcc agaacaattg ttgatcaaaa tcttgtaata tcgcacactt
    13201 ggatgctatt tgttgtagta aacctgcata ccgtggactg ccatcacgag caacattata
    13261 caaacactga ctaaaagcat cgaacaaaca atagatgtga tcactagtga aaaaaggtct
    13321 taattttttt tgttcttcgt cgctgtaatc caggagcatc tcattgattt cttcctgtga
    13381 gaattttaag acaaaattat cttcaggatt tgtttctttg ttgttttttt caatattctt
    13441 ctcctcaatg ctattcgcat tttgtttcgc aattatttcc gaattttttt tcgcagtttt
    13501 ttccgcagtt tttttcgcaa tttcttctgc aattctttcc cgagttttaa attttaaact
    13561 gacaccatcg atgtcaaccg gtgttgtttt aagatctctc tgcgcgtctt tcaaatttaa
    13621 ctcatagagt ttgggcagtt tatctgcaga tacgtatccg gatgaagaaa tctggtgtgt
    13681 acgcaggggc ggctgttcac aaagcatcga ggttatatcg tctttaaact cttttacatt
    13741 aaacttctcg gcattaacct tccctgttat aaaccccgca tcggtaattt tcttctctaa
    13801 ttgttttgta cg
//
LOCUS       DAPGOF010000127        13736 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.128, whole genome shotgun sequence.
ACCESSION   DAPGOF010000127 DAPGOF010000000
VERSION     DAPGOF010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.128"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(73..963)
                     /gene="rarD"
                     /locus_tag="RHV13_003357"
     CDS             complement(73..963)
                     /gene="rarD"
                     /locus_tag="RHV13_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDV1242863.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(1015..1482)
                     /locus_tag="RHV13_003358"
     CDS             complement(1015..1482)
                     /locus_tag="RHV13_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HDV1242864.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1647..2516
                     /gene="pldA"
                     /locus_tag="RHV13_003359"
     CDS             1647..2516
                     /gene="pldA"
                     /locus_tag="RHV13_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HDV1242865.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2649..4478
                     /gene="recQ"
                     /locus_tag="RHV13_003360"
     CDS             2649..4478
                     /gene="recQ"
                     /locus_tag="RHV13_003360"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HDV1242866.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4542..5162
                     /gene="rhtC"
                     /locus_tag="RHV13_003361"
     CDS             4542..5162
                     /gene="rhtC"
                     /locus_tag="RHV13_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HDV1242867.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTAVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5224..5844)
                     /gene="rhtB"
                     /locus_tag="RHV13_003362"
     CDS             complement(5224..5844)
                     /gene="rhtB"
                     /locus_tag="RHV13_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HDV1242868.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            5955..6977
                     /gene="pldB"
                     /locus_tag="RHV13_003363"
     CDS             5955..6977
                     /gene="pldB"
                     /locus_tag="RHV13_003363"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HDV1242869.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHTIVDFFNRHNSPSG
                     NRSTEV"
     gene            6985..7785
                     /gene="yigL"
                     /locus_tag="RHV13_003364"
     CDS             6985..7785
                     /gene="yigL"
                     /locus_tag="RHV13_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HDV1242870.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            7861..8760
                     /gene="bioP"
                     /locus_tag="RHV13_003365"
     CDS             7861..8760
                     /gene="bioP"
                     /locus_tag="RHV13_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HDV1242871.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8648..9601)
                     /gene="metR"
                     /locus_tag="RHV13_003366"
     CDS             complement(8648..9601)
                     /gene="metR"
                     /locus_tag="RHV13_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HDV1242872.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSSMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9837..12098
                     /gene="metE"
                     /locus_tag="RHV13_003367"
     CDS             9837..12098
                     /gene="metE"
                     /locus_tag="RHV13_003367"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDV1242873.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPARHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLDEVDEALALGHKVKPV
                     LLGPITWLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSGWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12383..12628)
                     /locus_tag="RHV13_003368"
     CDS             complement(12383..12628)
                     /locus_tag="RHV13_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDV1242874.1"
                     /translation="MITETEINVLKVMAEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVTEKGYNFVKSENK"
     gene            complement(12618..>13736)
                     /gene="hcp"
                     /locus_tag="RHV13_003369"
     CDS             complement(12618..>13736)
                     /gene="hcp"
                     /locus_tag="RHV13_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HDV1242875.1"
                     /translation="GEQQGCISSRCGTSASIGNRWQIGHEDEIFAFSLSNSITNTGKG
                     SQLHGLSFCKLIDKSSPLLINAINNNEQLFMEFDFYRINRFGRWEKYYNIQLRGALLS
                     AINHLFTENNLDTEAITVSYEYILSRHLIANTEFSYLAFPDNYNRLFIPRPKTKAGNG
                     FKTLNSKAVGRLLAAGGIYNGNIEGFRETADKLGGDAPAGYDQFMDNKGLLIAGASIV
                     AGAVLGRMRLPDLESLEHFGARGAVSGRPFNPDLAGGPIENLTTDGVTINREGIAIVE
                     KHIARFGHDPVNEVMINRLKDIEKGKIPPEQVDLNFYTHECREYQRYCNLGWETGQPD
                     GDAGYALWNHTHTATLEDYKLKGELNDLYHQDALDYDN"
BASE COUNT         3259 a         3463 c         3615 g         3399 t
ORIGIN      
        1 tctcctgtca acatggcaat atgcccactc tcatacaaga gtcggcatat tttttcagaa
       61 tatatttatt ttttatttgg acgttctacg ctgcgtataa atcgcatcca tcacaaaaat
      121 tgccagcgcc acccaaataa aggcgaaagt caccatctta tcggcacccg gtttttcacc
      181 ataaaacgtc acagccagca ggaacatcag cgtcgggcca atgtactgga aaaagcctaa
      241 cgttgagaga cgcaagcgcg tggcagcggc ggtaaaacac aacagcggta cggtagtgac
      301 aataccggcg gcgatcagca gtaaattcag cgacatcggg ttttgcccca tatggctggt
      361 tgagctgtcg gcaatagcaa acaggtaaat cgccgccacg ggcagcagcc acatggtttc
      421 aattaacatg ccggtttgtg cttctaccgc aatcttcttg cgcaccaggc cgtagaaggc
      481 aaaactaaat gccagcccca gcgcgataat aggtagcgaa ccaaaagtcc acagctggac
      541 caacacgcca catatcgcca gaatcaccgc cagccattgc atccggcgga atcgctcgcc
      601 gaggaaaatc atccccagca caatgttcac cagcggatta ataaagtaac caaggctcgc
      661 ttccagcata tggtgattgt tcaccgccca gataaacagt agccagttgc caccaatcag
      721 cacggcagag actgccagca taaaaatttt ctgtggcgtc tgaatcagcg tttttaaata
      781 ggaccactgg cggcaaatgc tcatcagcac caccataaag aaaaacgacc agatcacgcg
      841 atgcgtcagg atttcatcgg cgggcacgta gtaaatcaac ttgaagtacg ctggggctat
      901 accccaaata aaataagcgg caagagcgag taatacgccc tgccgcgttt gttttgcatc
      961 catcgggaat actcattttt aattggtaac agcagtttac ctgcttttac gttatcaacc
     1021 taccatatag gtggcggtgg cactggcaat ataaagctgt tcttcattgt gtaattcaac
     1081 gcgggcgacg gcgactttat tgcctgcacg caacaggcta ctggttgcag taaaacgctc
     1141 gcccctgcct gggcgcagat aatcaacgcg aagatcaatg gtccccatcc gcgatagccg
     1201 ctggcgtagt tcatcttcac tgatggtttc gtggcgggtt aaggtacttc ccacgcacac
     1261 cagacctgcg gcgacatcca gcgccgacgc aatgaccccg ccgtgcaaaa tgctttgcgc
     1321 ccagttgccc accatcattg gctgattttt aaaggccagc tgtgcgaact ctttttcgta
     1381 acgctccagt tccatcccca atgcgcggtt aaatggcatg tgataaacaa acatctcacc
     1441 cactaatttc agggcttgtt cagcggtcag tacggcagac atatcatcct tacacttcat
     1501 tggttaatga aatgttgatt ttatgcttct ttgttgttgg tttctacttt aggaagggat
     1561 aactaacggc tatggagtta agtatgtaaa atagcccgca gaaaaatatt caccttatca
     1621 ataattcgtt acggagaaca cgaccgatgc ggactctgca gggctggttg ttgccggtgt
     1681 ttatgttgcc tatggcagta tatgcacaag aggcaacggt gaaagaggtg catgacgcgc
     1741 cagcggtgcg tggcagtatt atcgccaata tgctgcagga gcatgacaat ccgttcacgc
     1801 tctatcctta tgacaccaac tacctcattt acacccaaac cagcgatctg aataaagaag
     1861 cgattgccag ttacgactgg gcggaaaatg cgcgtaagga tgaagtaaag tttcagttga
     1921 gcctggcatt tccgctgtgg cgtgggattt taggcccgaa ctcggtgttg ggtgcgtctt
     1981 atacgcaaaa atcctggtgg caactgtcca atagcgaaga gtcttcaccg tttcgtgaaa
     2041 ccaactacga accgcaattg ttcctcggtt ttgccaccga ttaccgtttt gcaggctgga
     2101 cgttgcgcga tgtggagatg gggtataacc acgactctaa cgggcgttca gacccgacct
     2161 cccgcagctg gaaccgcctt tatactcgcc tgatggcaga aaacggtaac tggctggtag
     2221 aagtgaagcc gtggtatgtg gtgggtaata ctgacgataa cccggatatc accaaatata
     2281 tgggttacta ccagcttaaa atcggctatc acctcggtga tgcggtgctc agtgcgaaag
     2341 gacagtacaa ctggaacacc ggctacggcg gcgcggagtt aggcttaagt tacccgatca
     2401 ccaaacatgt gcgcctttat actcaggttt acagcggcta tggcgaatcg ctcatcgact
     2461 ataacttcaa ccagacccgt gtcggtgtgg gggttatgct aaacgatttg ttttgatgaa
     2521 cggttgagtg gttggcaaat ctggaatcca gcatccagga ttaccctctc agagactaaa
     2581 agcattgcag tttctcgcgc aggcgctgaa aatagcgcct gtttttattt caggcaatcg
     2641 gggtgaatgt ggcgcaggcg gaagtgttga atctggagtc cggagctaaa caggttttac
     2701 aagaaacctt tggctaccaa cagtttcgcc ccggccagga agaaattatc gacactgtgc
     2761 tttccggtcg cgattgcctg gtcgtcatgc ccaccggtgg cggaaaatcc ctttgctatc
     2821 aaattcctgc cttattgcta aacggcctta ccgtggttgt ttcaccgctg atttcgttga
     2881 tgaaagatca ggtggatcaa ctgcaagcca acggcgtggc ggcggcgtgc cttaactcga
     2941 cgcaaacccg cgaacagcaa cttgaagtga tgacaggctg ccgcaccggg caaattcgtc
     3001 tgctttatat cgccccggaa cgcctgatgc tggataactt tctggagcat ctggcgcact
     3061 ggaatccggt gttattagcc gttgatgaag cgcactgtat ctcccaatgg ggccacgatt
     3121 tccgcccgga atatgccgcg ctcggtcagt tgcgccagcg gttcccgacg ctgccgttta
     3181 tggcgctgac cgccacagcc gacgacacca cgcgccagga tatcgtgcgc ctgctggggc
     3241 tgaacgatcc gctgattcaa atcagcagtt ttgaccgtcc gaatattcgc tacatgctga
     3301 tggagaaatt caaaccgctc gatcagttga tgcgctacgt gcaggaacag cgcggtaagt
     3361 ccggcattat ctactgcaac agccgggcga aagtagaaga caccgctgcg cgcctgcaaa
     3421 gcaagggtat tagcgcggcg gcctatcatg ccgggctgga aaataatgtc cgcgccgacg
     3481 tgcaggagaa attccagcgc gatgacctgc aaattgtggt ggcgacggtg gcgttcggca
     3541 tgggcatcaa taaaccaaac gttcgcttcg tggtccactt tgatattccg cgcaatatcg
     3601 aatcctatta tcaggaaacc ggtcgcgccg ggcgtgatgg cctgcctgcg gaagcgatgc
     3661 tgttttacga tccggctgat atggcgtggc tgcgccgttg tctggaagag aagccgcagg
     3721 ggcagttgca ggatatcgag cgccacaaac tcaatgcgat gggcgcgttt gccgaagcgc
     3781 aaacttgccg tcgtctggta ttgctgaact attttggcga agggcgtcag gagccgtgcg
     3841 ggaactgcga tatctgcctc gatccgccga aacagtacga cggttcaacc gatgctcaga
     3901 ttgccctttc caccattggt cgtgtgaatc agcggtttgg gatgggttat gtggtggaag
     3961 tgattcgtgg tgctaataac cagcgtatcc gcgactatgg tcatgacaaa ctgaaagtct
     4021 atggcatggg ccgtgataaa agccatgaac attgggtgag cgtgatccgc cagctgattc
     4081 acctcggcct ggtgacgcaa aatattgccc agcattctgc cctacaactg acagaggccg
     4141 cgcgcccggt gctgcgcggc gaatcctctt tgcaacttgc cgtgccgcgt atcgtggcgc
     4201 tcaaaccgaa agcgatgcag aaatcgttcg gcggcaacta tgatcgcaaa ctgttcgcca
     4261 aattacgcaa actgcgtaaa tcgattgccg atgaaagtaa tgtcccgccg tacgtggtgt
     4321 ttaacgacgc aaccttgatt gagatggctg aacagatgcc gatcaccgcc agcgaaatgc
     4381 tcagcgttaa cggcgttggg atgcgcaagc tggaacgctt tggtaaaccg tttatggcgc
     4441 tgattcgtgc gcatgtcgat ggcgacgacg aagagtagtc agcagcataa aaaagtgcca
     4501 ggatgaagac tccgtaaacg tttcccccgc gagtcaaatg tatgttgatg ttatttctca
     4561 ccgtcgccat ggtgcacatt gtggcgctta tgagtcccgg tcccgatttc ttttttgtct
     4621 ctcagaccgc ggtcagtcgt tcccgtaaag aagcgatgat gggcgtgctg ggtattacct
     4681 gcggcgttat ggtttgggct gggattgcgt tgcttggcct gcatttgatt atcgaaaaaa
     4741 tggcctggct gcatacgctg attatggttg gcggtggcct gtatctctgc tggatgggtt
     4801 accagatgct gcgcggcgcg ctgaaaaaag aggcggtttc tgcacctgcg ccacaggtcg
     4861 agctggcgaa aagtgggcgc agtttcctga aaggtttact gaccaatctc gctaatccga
     4921 aagcgattat ctattttggt tcggtattct cattgtttgt cggcgataac gtcggcacta
     4981 ccgcgcgctg gggcattttc gcgttgatta ttgtcgaaac gctggcgtgg tttactgcgg
     5041 tggccagcct gtttgccctg ccgcaaatgc gccgtggtta tcaacgtctg gcgaagtgga
     5101 ttgatggttt tgccggggcg ttatttgccg gatttggcat tcatttgatt atttcgcggt
     5161 gatgccagac gcgtcttcag agtaagtcgg ataaggcgtt tacgccgcat ccgacattat
     5221 ttttcacgca tgcctcgccg atgctaacag cgctcccacc agcataaaca acgagccgaa
     5281 aatcttattc agcgccttca tctgctttgg tcctttaatc catagagcaa tccgttgagc
     5341 aagggtggcg taaccgatca tcacaataat atcgaccaca atagtggtga cgccgagcac
     5401 gatatactgc atcagttgcg gctgttgcgg catgatgaat tgcggaaata gcgccgccag
     5461 aaacacaata cttttgggat tggtgagatt cacaaaaact gcgcgctgga acaaatgtcg
     5521 acgcgattga gtagaggcca gcgatttaag atcaattgcg ccagcggcgc gccattgctg
     5581 tattcccagc caaatcaagt aagccgcgcc tgcccacttc aacacttcaa acgcaatcac
     5641 tgagcgggaa aatagcgtcc ccaaccccac gccaaccagc acaatatgaa tcgccagtcc
     5701 ggtctgaagc ccagcaatag acgccaccgc gccgcgataa ccgtggttga gcgaggtggt
     5761 catagtgttg attgcaccag agcctggcga caggcttaaa atgatcgatg tcagcaggta
     5821 ggcaaaccac cattctaagg tcatgatgaa ctcccggtgt gtctattttt gtgccacaat
     5881 acgctactgt cgcagcgttg tgtcaggcac gctaaaaaaa acgattttac gtggtttaag
     5941 aggcagatta cccgatgttt cagcagcaaa aagactggga aacaagagaa aacgcgtttg
     6001 ctgcttttac catgggaccg ctaactgatt tctggcgtca gcgtgatgaa gcagagttta
     6061 ctggtgtgga tgacattccg gtgcgctttg tccgttttcg cgcacagcac catgaccggg
     6121 tggtagtcat ctgcccgggg cgtattgaga gctacgtaaa atatgcggaa ctggcctacg
     6181 atttgttcca tttggggttt gatgtcttaa tcatcgacca tcgcgggcag ggacgttccg
     6241 gtcgcctgtt agccgatccg catctcgggc atgttaatcg ctttaatgat tatgttgatg
     6301 atctggcggc attctggcaa caggaggtcc agcccggtcc gtggcgtaaa cgctatatac
     6361 tggcacattc gatgggcggt gcgatctcca cattatttct gcaacgccat ccaggtgtat
     6421 gtgacgccat tgcgctaact gcgccaatgt ttgggatcgt gattcgtatg ccgtcattta
     6481 tggcacggca gatcctcaac tgggccgaag cgcatccacg tttccgtgat ggctatgcaa
     6541 taggcaccgg gcgctggcgc gcgttgccgt ttgctatcaa cgtactgacc cacagcagac
     6601 agcgatatcg acgtaactta cgcttctatg ctgatgaccc aacgattcgc gtcggtgggc
     6661 cgacctacca ttgggtacgc gaaagtattc tggctggcga acaggtgtta gccggtgcgg
     6721 gtgatgacgc cacgccaacg cttctcttgc aggctgaaga ggagcgcgtg gtggataacc
     6781 gcatgcatga ccgtttttgt gaactccgca ccgccgcggg ccatcctgtc gaaggtggac
     6841 ggccgttggt aattaaaggt gcttaccatg agatcctttt tgaaaaggac gcaatgcgct
     6901 cagtcgcgct ccacaccatc gttgattttt tcaacaggca taactcaccc agcggaaacc
     6961 gctctacaga ggtttaaatt tcttatgtac caggttgttg cgtctgattt agatggcacg
     7021 ttactttctc ccgaccatac gttatcccct tacgccaaag aaactctgaa gctgctcacc
     7081 gcgcgcggca tcaactttgt gttcgcgacc ggtcgtcacc acgttgatgt ggggcaaatt
     7141 cgcgataatc tggagattaa gtcttacatg attacctcca atggcgcgcg cgttcacgat
     7201 ctggatggca atctgatttt tgctcataac ctggatcgcg acattgccag cgatctgttt
     7261 ggcgtagtca acgacaatcc ggacatcatt actaacgttt atcgcgacga cgaatggttt
     7321 atgaatcgcc atcgcccgga agagatgcgc ttttttaaag aagcggtgtt caaatatgcg
     7381 ctgtatgagc ctggattact ggagccggaa ggcgtcagca aagtgttctt cacctgcgat
     7441 tcccatgaac aactgctgcc gctggagcag gcgattaacg ctcgttgggg cgatcgcgtc
     7501 aacgtcagtt tctctacttt aacctgtctg gaagtgatgg cgggcggcgt ttcaaaaggc
     7561 catgcgctgg aagcggtggc gaagaaactg ggctacagcc tgaaggattg tattgccttt
     7621 ggtgacggga tgaacgacgc cgaaatgctg tcgatggcgg ggaaaggctg cattatgggc
     7681 agtgcgcacc agcgtctgaa agatctgcat ccagagctgg aagtgattgg tactaatgcc
     7741 gacgacgcgg tgccgcatta tctgcgtaaa ctctatttat cgtaatcgtt ctttatttgg
     7801 tcagttgtca acctgatact tcgctacaat ggatactcgt taatcaaaga gttttccatt
     7861 gtggcgctac ttatcatcac cacgattttg tgggccttct cctttagttt ttatggcgag
     7921 taccttgcgg ggcacgtcga tagctatttt gcggtgctgg tgcgcgttgg cctggcggca
     7981 ctcgtttttc tgccgtttct gcgtacccgt ggcaatagcc tgaaaacagt cggcctgtat
     8041 atgctggtgg gcgcgatgca gctcggcgtg atgtatatgc tgagtttccg cgcttatctc
     8101 tacctgacgg tttccgaact gctgctgttc accgtactga cgccgctcta catcacgctg
     8161 atttatgaca tcatgagtaa gcgccgtctg cgctggggat atgcttttag tgccttgttg
     8221 gcggtgattg gtgccgggat tattcgctat gatcaggtca ccgaccattt ctggactggc
     8281 ttgttgctgg tgcaactctc caatatcact tttgccatcg gcatggtggg ttacaaacgc
     8341 ctgatggaaa ctcgcccgat gccgcagcat aacgcctttg cgtggttcta tcttggcgcg
     8401 ttcctggtgg cggtaattgc atggttcttg ctgggaaatg cgcagaaaat gccgcaaacc
     8461 acgttgcaat ggggcattct ggtgtttctt ggcgtggtgg cttccgggat tggctacttt
     8521 atgtggaact acggcgcgac gcaggtggac gccggaacgc tgggaattat gaataatatg
     8581 cacgttccgg cagggctgct ggtaaacctg gctatctggc accaacagcc gcactggcca
     8641 acgtttatta caggcgcgct ggtgatcctg gcctcactgt gggtgcatcg taagtgggtc
     8701 gctccgcgct cttcacaaac ggcagatgat cgcaggcgtg attgcgcgct gagcgaataa
     8761 acgcttccgt aactggctga cgctgctcgc catcgcgcac ggcggcgtac agtcggctcc
     8821 acaagccttc gcccagggtt ttggtcacca ccagaccctg gcgctcaaaa ctctctacta
     8881 cccaatgcgg cagggcggca atacccatcc gcgcggcaac catctgaatc aacaataagg
     8941 tgttatcgac gcttttcagt gacgggctga cgcctgccgg ctgaagaaaa tgccgccaga
     9001 catccagtcg gctacgctgc accggataaa tcaacagcgt ctcgctggcg agatcttccg
     9061 gtgtaatacg cgtttttgcc gccagtggat ggtcaggtgc taacaccagg cgtacttcat
     9121 agtcgaacat cgacgaataa tgcaggccac tgcgcggcag aatatcggag gtcatcacca
     9181 gatccagctc gccctgttgc aacgcaggct gcgggtcaaa cgtcacgccc gatttaaaat
     9241 ccatctctac ctgcggccag ttcttatgga aattttctaa cgcgggcgtc agccactgaa
     9301 tacagctatg gcactcaatg gcaatgcgca gacgcgtctg ctgcggttca ttgcaggctt
     9361 gcagtgcctg gctaatttgc ggcagcacct ggtttgccag ttgcaacagg atttctccct
     9421 gcggtgtaaa gcgaagcggc tggcttttac gcacaaatag ccggaagcca aggcgttgtt
     9481 ccagatcgct aaactggtga gacagggcgg attgcgtctg atgcaacgtc gccgcagcgg
     9541 ctgcgagcga gccgcagttc cgcaacgctt gtagcgtttt caggtgtttt acttcgatca
     9601 tgaaagtcct tcacttcggc atgaataatt tgcgcttgag gaatatacag taaccgccaa
     9661 ttatggatgt gtaaacatct ggacggctaa aatccttcgt cttttaaatt tatggtgcgt
     9721 tggctgcgtt tctccacccc ggtcacttac ttcagtaagc tcccggggat gaataaactt
     9781 gccgccttcc ctaaattcaa aatccatagg atttacatat aattagagga agaaaaatga
     9841 caatattgaa tcacaccctc ggtttccctc gcgttggcct gcgtcgcgag ctgaaaaaag
     9901 cgcaagagag ttattgggcg gggaactcca cgcgtgaaga actgctggcg gtagggcgtg
     9961 aattgcgtgc tcgtcactgg gatcaacaaa agcaagcggg tatcgacctg ctgccggtgg
    10021 gcgattttgc ctggtacgat catgtactga ccaccagtct gctgctgggt aacgttccgg
    10081 cgcgtcatca gaacaaagat ggttcggtag atatcgacac cctgttccgt attggtcgtg
    10141 gacgtgcgcc gactggcgaa cctgcggcgg cagcggaaat gaccaaatgg tttaacacca
    10201 actatcacta catggtgccg gagttcgtta aaggccaaca gttcaaactg acctggacgc
    10261 agctgctgga cgaagtggac gaggcgctgg cgctgggcca caaggtgaaa cctgtgctgc
    10321 tggggccgat tacctggctg tggctgggga aagtgaaagg tgaacagttt gaccgcctta
    10381 gcctgctgaa cgacattctg ccggtttatc agcaagtgct ggcagaactg gcgaaacgcg
    10441 gcatcgagtg ggtacagatt gatgaacccg cgctggtact ggaactacca caggcgtggc
    10501 tggacgcata caaacccgct tacgacgcgc tccagggaca ggtgaaactg ctgctgacca
    10561 cctattttga aggcgtaacg ccaaatctcg acacgattac tgcgctgcct gttcagggtc
    10621 tgcatgttga cctcgtacat ggcaaagatg acgttgccga actgcacaag cgtctacctt
    10681 ctggctggct actgtctgct ggtcttatca atggtcgtaa cgtctggcgc gccgatctta
    10741 ctgagaaata tgcgcaaatt aaggacattg tcggcaaacg tgatttgtgg gtggcatctt
    10801 cctgctcgtt gctgcacagc cccatcgacc tgagcgtgga aacgcgtctt gatgcagaag
    10861 tgaaaagctg gtttgccttc gccctacaaa aatgccatga actggcactg ctgcgcgatg
    10921 cgctgaacag tggtgacacg gcagctctgg cagagtggag cgccccaatt caggcgcgtc
    10981 gtcactctac tcgtgtacat aatccggcgg tagaaaagcg tctggcggcg atcaccgccc
    11041 aggacagcca gcgtgcgaat gtctatgaag tgcgtgctga agcccagcgt gcgcgtttta
    11101 aactgccagc gtggccgacc accacgattg gttccttccc gcaaaccacg gaaattcgta
    11161 ccctgcgtct ggatttcaaa aagggcaatc tcgacgccaa taactaccgc acgggcattg
    11221 cggaacatat caggcaggcc attgttgaac aggaacgttt gggactggat gtgctggtac
    11281 atggcgaggc cgagcgtaat gacatggtgg aatactttgg cgagcacctc gacgggtttg
    11341 tctttacgca aaacggttgg gtacagagct acggttcccg ctgcgtgaag ccgccgattg
    11401 tcattggtga cgttagccgc ccggcaccga ttaccgtgga gtgggcgaag tatgcgcaat
    11461 cgctgaccga caaaccggtg aaagggatgc tgaccggccc ggtgacgatt ctctgctggt
    11521 cgttcccgcg cgaagatgtc agccgtgaaa ccatcgccaa acagattgcg ctggcgttgc
    11581 gtgatgaagt ggcggacctg gaagccgcag gaattggcat catccagatt gatgaaccgg
    11641 cgctgcgcga aggtttaccg ctgcgtcgta gcgactggga tgcatatctc cagtggggcg
    11701 tggaggcctt ccgtatcaac gccgccgtgg cgaaagatga cacacaaatc cacactcaca
    11761 tgtgttattg cgagttcaac gacatcatgg attcgattgc ggcactggat gcagacgtca
    11821 tcaccatcga aacctcgcgt tccgacatgg agttgctgga gtcgtttgaa gagtttgatt
    11881 atccgaatga aatcggtcca ggcgtctatg acattcactc gccaaacgta ccgagcgtag
    11941 aatggattga agccttgctg aagaaagcag caaaacgcat tccggcagag cgcctgtggg
    12001 ttaacccgga ctgtggcctg aaaacgcgcg gctggccaga aacccgcgcc gcgctggcga
    12061 acatggtaca ggcggcgcag aatttgcgtc ggggatgatg aatattaacg aatcactgta
    12121 aattgcagta cgatgaaaga ggcaaaactg cctgatacca ggcctacata atttatgcaa
    12181 tttattgaat ttttgggttt tgttggctgg acaaggcgtt cacgccgcat ccggcatgaa
    12241 caacgcgcac tttgccaata atctgatggc ttccatcacc ggaagccatt ttgctatctg
    12301 cgcccgttca gatacagaaa taaccgaatc atttttacgc aaaatataat cctgagcgat
    12361 ttgttcagta atcaacgcta cgttatttat tctcactttt cacgaaattg tatccctttt
    12421 cagtaacgtg gtagtgaggc atagacttat gtccgctatc ctcaataata accaaaccct
    12481 cgttagctaa tttgttaaca atgttaacaa cattaccgaa tcctggaatt tgccttatgg
    12541 atagtgtgcg atcgagattc atccagttcc agtttatatc tttctcagcc ataactttca
    12601 gtacgttaat ttcagtttca gttatcatag tcaagtgcat cctggtggta taagtcatta
    12661 agttctccct taagtttata atcttctaga gtagcagtat gcgtatgatt ccagagtgca
    12721 tagcctgcgt caccatcagg ttgtcctgtt tcccaaccaa gattacagta acgctgatat
    12781 tcccgacact catgagtata gaagttaaga tctacttgtt ctggggggat ttttcctttc
    12841 tcaatgtctt tcagtctatt gatcatcact tcatttacag ggtcatgacc aaatctggct
    12901 atgtgttttt caacaatagc gataccttcc ctgttgattg ttacgccatc tgtcgtcaga
    12961 ttttctattg gaccaccggc aaggtcggga ttaaatggtc ttcctgatac tgctcctctg
    13021 gctccgaaat gctcaagtga ttccaaatca gggagtctca ttctccccag aactgctcca
    13081 gcaactattg aagcaccagc gatgagtaac cctttgttat ccataaattg atcgtaacca
    13141 gcaggtgcat ctccccccaa cttgtctgct gtttccctga atccttcgat attcccattg
    13201 tatatgccac cagcagcaag cagtctgcca accgctttac tgttaagtgt tttgaaacca
    13261 ttaccagctt tagtttttgg ccgtggtata aacagacggt tgtagttatc gggaaatgcc
    13321 aaataactaa attcagtatt agcaatcaga tgcctggaga gtatatattc ataactgaca
    13381 gtgatggctt ctgtatccag attgttctca gtaaacaggt gattgattgc tgataagagc
    13441 gcgccgcgga gttgaatgtt gtaatatttc tcccatcgac caaatctatt gatccgatag
    13501 aagtcaaact ccatgaacaa ctgctcgtta ttgttaatag cgttaataag taacggggag
    13561 cttttgtcaa tcaatttaca gaaactaagg ccatgcaatt gtgacccttt gcctgtattc
    13621 gtaatactat tagagagcga gaacgcgaag atttcatctt catggccaat ctgccagcga
    13681 ttacctattg atgcgctcgt tccacaacga gaggaaatac atccttgttg ttctcc
//
LOCUS       DAPGOF010000128        13636 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.129, whole genome shotgun sequence.
ACCESSION   DAPGOF010000128 DAPGOF010000000
VERSION     DAPGOF010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13636
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.129"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..741)
                     /locus_tag="RHV13_003370"
     CDS             complement(<1..741)
                     /locus_tag="RHV13_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN/FAD transporter"
                     /protein_id="HDV1242876.1"
                     /translation="MLRHILTAKNLLSNPIFKFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSLFEMWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALALTYLEL
                     TVLSYPAAAITLIGSGALRGAGNTKIPLL"
     gene            746..821
                     /locus_tag="RHV13_003371"
     tRNA            746..821
                     /locus_tag="RHV13_003371"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:779..781,aa:Asn,seq:gtt)
     gene            complement(859..1809)
                     /gene="cbl"
                     /locus_tag="RHV13_003372"
     CDS             complement(859..1809)
                     /gene="cbl"
                     /locus_tag="RHV13_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HDV1242877.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFVRKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRKVMENSEEEIDYQI"
     gene            complement(1911..2828)
                     /gene="nac"
                     /locus_tag="RHV13_003373"
     CDS             complement(1911..2828)
                     /gene="nac"
                     /locus_tag="RHV13_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HDV1242878.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAIRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVMSSPVMEKRQWQLVS"
     gene            3154..3229
                     /locus_tag="RHV13_003374"
     tRNA            3154..3229
                     /locus_tag="RHV13_003374"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3187..3189,aa:Asn,seq:gtt)
     gene            complement(3285..4220)
                     /gene="ldtA"
                     /locus_tag="RHV13_003375"
     CDS             complement(3285..4220)
                     /gene="ldtA"
                     /locus_tag="RHV13_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDV1242879.1"
                     /translation="MMRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSFTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(4282..5361)
                     /gene="cobT"
                     /locus_tag="RHV13_003376"
     CDS             complement(4282..5361)
                     /gene="cobT"
                     /locus_tag="RHV13_003376"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HDV1242880.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDSAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFCVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKINVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLDMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(5373..6116)
                     /gene="cobS"
                     /locus_tag="RHV13_003377"
     CDS             complement(5373..6116)
                     /gene="cobS"
                     /locus_tag="RHV13_003377"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HDV1242881.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(6113..6658)
                     /gene="cobU"
                     /locus_tag="RHV13_003378"
     CDS             complement(6113..6658)
                     /gene="cobU"
                     /locus_tag="RHV13_003378"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HDV1242882.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWRTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            6804..7016
                     /locus_tag="RHV13_003379"
                     /pseudo
     CDS             6804..7016
                     /locus_tag="RHV13_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280452.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
     gene            complement(7117..7446)
                     /locus_tag="RHV13_003380"
     CDS             complement(7117..7446)
                     /locus_tag="RHV13_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HDV1242883.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(7618..8676)
                     /locus_tag="RHV13_003381"
     CDS             complement(7618..8676)
                     /locus_tag="RHV13_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDV1242884.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(8875..9348)
                     /gene="sbmC"
                     /locus_tag="RHV13_003382"
     CDS             complement(8875..9348)
                     /gene="sbmC"
                     /locus_tag="RHV13_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HDV1242885.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPGYFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(9467..10633)
                     /gene="dacD"
                     /locus_tag="RHV13_003383"
     CDS             complement(9467..10633)
                     /gene="dacD"
                     /locus_tag="RHV13_003383"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HDV1242886.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNIDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIALGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            10842..12269
                     /gene="sbcB"
                     /locus_tag="RHV13_003384"
     CDS             10842..12269
                     /gene="sbcB"
                     /locus_tag="RHV13_003384"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HDV1242887.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNIFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFEYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDTQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            complement(12312..12539)
                     /locus_tag="RHV13_003385"
     CDS             complement(12312..12539)
                     /locus_tag="RHV13_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HDV1242888.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(12553..13611)
                     /gene="yeeE"
                     /locus_tag="RHV13_003386"
     CDS             complement(12553..13611)
                     /gene="yeeE"
                     /locus_tag="RHV13_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HDV1242889.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISIQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTVRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
BASE COUNT         3668 a         3618 c         3058 g         3292 t
ORIGIN      
        1 caatagcggt attttcgtat tccctgcacc acgaagtgcc ccgctaccaa taagagtgat
       61 ggcagctgct ggataactga gtaccgtcag ctccagataa gtcaacgcca gtgctttaac
      121 ttctgtcgtg gcatcacccg cgacgaaatc aataatttgt tcgccaaaat gatgaataag
      181 cgttgccaac agtacggcaa acaacgtcat gatcaccaat gactgccgcg tcgccaccct
      241 cgctcgtcgt cgatcccgct taccgagact aaatgccaca acgacagtag taccaagatc
      301 gatagcagca aaaaaagcca taatgaccat attgaagctg tccgccaatc ccacgccggc
      361 catcgcatct tttcccagcc agctgaccag aaaagtgctc agaaccccca tcaacaggac
      421 acaggcattc tccatgaaga taggaacagc aagcggggtt atctcgcgcc agaacaacac
      481 tttgtagctc ttgcgtttag cgtgccagcg agtgccgtga acaacctggc gtaaagcaga
      541 ggagatattc aaagccgacc ttaattgcag aaagtgaaac cacatttcaa ataatgaggg
      601 agaatcagca aagctgcaaa gattttcgcc aacaaattgt ctgcaaatgc aacaaactgt
      661 tgatagaaac ggcaaacagt tgggaaattt aaaaatcggg tttgacaaaa gatttttcgc
      721 cgttaagatg tgcctcaaca gcgattcctc tgtagttcag tcggtagaac ggcggactgt
      781 taatccgtat gtcactggtt cgagtccagt cagaggagcc aaatttctga aaagcccgct
      841 tttatagcgg gctttttgct atatctgata atcaatttcc tcttcactgt tctccatcac
      901 cttgcgcttg atatcctcaa ctgacagtcc tgcattacaa agttccagaa agcgccagac
      961 atagttacgt tgaagttgcc ctcgcttcag tcccaaccat acagtattgg catcgaaaag
     1021 atgccgcgta tccagacgga ttaaattctc ttcctcttgt tcgccgctgg attgttcggc
     1081 aactaatcca atccccagcc caagagcaac ataggtttta atgacatcag aatcctgcgc
     1141 acttaataca atatctgcca gcaaaccttt gcggacaaat gcgtcatcaa tacgtgagcg
     1201 ccccgtaatc ccctgtcggt aagtaattaa cggccacttc gctattgatt ccagcgtcaa
     1261 cggtgtaatt tgcgtcaagg gatgatcgag tggaacaagc aaactatggt gccaacgaaa
     1321 ccacgggaag gcgacgagct gcgggtcatt actcaaacgc tcgctggcga taccaatatc
     1381 agcttcgcca ttttgcaaca acgtcgcaat ttcctgtggc gtcccctgga ttagctcgag
     1441 ccgaacctcc gggaaaagtt cgcgaaaagc tttaatgacc tctggcaagc tataacgtgc
     1501 ctgagtatgc gtcgttgcaa tagtgagaac gccagacgta tcgttggtaa acaggtctgc
     1561 aagccgacga acattactgg cttcattcag aatacgttct gcaatgacca gtaatgcttt
     1621 gcccggttca gtcatgccca gcagtcgctt acctcgtcga acaaatattt cgatgccaag
     1681 ttcatcctcc agttcccgaa tatgacggct gacgcctgac tgtgaggtaa aaagcatatt
     1741 cgcaacctct gtcaggttgt aatcctgacg tgcagcctcg cggattatct ttagttgttg
     1801 gaaattcacg gtaaactccg ggcagttcag atttcccgtt attgttaaag tctaatgccc
     1861 ggcataacaa ataataaaaa cccgcatctt attccatccc gatataacgc ttagctcacc
     1921 aattgccact gccttttttc catcactgga gaactcatca ctgacattaa caactctttc
     1981 actgcttgcg cctgtggtga taagttcgct ctggcgggta aatttagtga caaagagaga
     2041 ctcatggaag gtgtggtaat gcgtgacatc cacccgttta ctgcgccaca taacgaacgc
     2101 gcggccgatt cgggtaatac tgcaacgccc atgccgctcg caatcgctgc ggtaagcgtg
     2161 gcaatagact caatttcacc aatgactttt gccgtgagtc gccgtaggga aaaagcctca
     2221 tcaacacgaa gtctaatagc actgtaatca ctggggagaa agaggttcat ttgcgcaata
     2281 gcattaacat caacgctttg ccccgggcaa tcttgagttc ctaccagaaa aagatcttct
     2341 ttcagtaaag cctgactgga tacaccagcc acaggggaat gctcataaat caccgccata
     2401 tcgagttggt gatttatcag tttttcgtta agcactgcac cgctattttc atgaagatag
     2461 ataacgacct ccggaaattc agcgcgaacc gcctgtaata agggcatggt gatggatgac
     2521 gcagcggttc ctggcgcaaa gccaatcgag acttgccccg ataatgcctg accaacgtta
     2581 tgcaccgcca gttgggcctg ctcacactga cgtaaaatgg cccgcgcatg ggtatagaga
     2641 atttttccgg cgtctgttgg cgtaacgcct cgttttgtgc gaatcaaaag ttgttgattt
     2701 aactcacctt ccagtgtggc aacctgctgg ctgagcgctg gttgtgcgat atgcaatact
     2761 tcagcagcct gggtcaggct accaatatct acaattttta cgaagtattt caggcgtctg
     2821 aagttcatgt tgcctccggt ttttgagaat cggcccaagt gccgccatta cttacaacca
     2881 gactgcaaga tgcttgccag ttttattttg gcgttgatgt acaagctaac caactgtcaa
     2941 ataagagatt atgatagatt cgtcattggc tcttttcacc agcagtcgcg ttcccctgcc
     3001 ctataaaagg agggtatgca ccacgatggt tcattaccaa taagattgaa atctcatcac
     3061 tttgttgaaa ttgacagcaa acaaacaaaa aaatgcattt caccctttga catcaccatg
     3121 cactgccatt aatatgcgcc ccgttcacac gattcctctg tagttcagtc ggtagaacgg
     3181 cggactgtta atccgtatgt cactggttcg agtccagtca gaggagccaa attcaaaaaa
     3241 gcctgctttc tagcaggctt tttgctttct aattaccaac gctcttaaaa catctgtctt
     3301 gaaccagaac taatttgcac aggcattccc gatcgacgtt gcaacgcagc atttgcgcga
     3361 ttcacatcaa cttcttgccc gttgataaac gcccgcaaag atggggttac cggcaatggc
     3421 acttttcggt cagactcata ttctgcacga ttgcgcgaca gcggctcatg aacttccagc
     3481 cagttcgagc catctggttc agtggtgtat tttactggct ggtcgataat ttgcacacgc
     3541 gtcccaacag gaacattatc aaacagatat ttgatatcgt cattgcgcag acgaatacag
     3601 ccctgactta cccggagccc aataccaaaa ttggcattgg taccgtggat ggcatacaac
     3661 ctgccaatat aaatcgcgta cagccccatg ggattatcgg gccccgcagg aacaaatgcg
     3721 ggcaaactct cccctcgttt cgcatattcg cgccgagtgt tcggcgttgg cgtccaggtt
     3781 ggagcttctt gtttacgttc aacggtagtc acccagttac gcggggtttc tcgcccagcc
     3841 tggccgatac caataggaaa gacttccaca gtattactgt ctggtgggta gtaataaaga
     3901 cgcatctcag cgacgttaac cacaatccct ttacgaacag tggcgggcaa aatcagttgc
     3961 tgcggaatgg tgagttgcga gccagacttc ggcaagaaaa catcagcgcc cgggttcgct
     4021 tccagcatgt tacttaaccc ttgcccgtat tgtgcggcaa aagtctccag cggctgggta
     4081 ttgtgatcag gaacagttac agtaaacgac tgccccacta aacggctacc ctctggaggt
     4141 aatggataag ttaccgccag gctagtatgg ctggcaaaaa gcagagcaaa tgagcaaaga
     4201 atatttacac gacgcatcat gtccctttcc taagtcgcga aagctatccg ttaagtatag
     4261 cttttatcag acttttcgtt tttaactgtt caaatcagaa gtcgtattcc ccggtagaac
     4321 aatattactg gcagcaagtt cgcccatgtt gttatatatc gcacaggcag cttcgatgat
     4381 gggcatcgcc agagctgcgc cactcccctc acctaaacgc atctccatat cgagataagg
     4441 ctccagcccc aaatgcgaca gcgctatacg cgcccctttt tctgccgaca ggtgagaagg
     4501 aatgagatac ggtttgattg caggagacat ctggcaggct gcgagcgcag cagcataaga
     4561 aagaaatcca tccagcaaca cgggtaaacc gcaggaagca gcgcctaaca tcacgccagc
     4621 cattccgacc aaatcaaatc cacccacttt tgccagaaca tcaacgccat cctgcggatt
     4681 tggttgattc aacgtaatcg cccggcgcac aacattaatt ttattagcca gtttatctgt
     4741 tggcaggttt gcgccaatcc caaccacttc ttcaggatcc cggccagtga ttgtgctgac
     4801 tattgccgct gccggtgtcg tgtttgccat ccccagttca cctacacaaa acagcgtgac
     4861 accatttttt gccagctccc gcgtataaca tatgacgtcc aaaagcaact tttcagcctg
     4921 acggcgactc attgccggag ctgaagcaat attgccgcta cctcgtgcga cacgcatgtt
     4981 gataagcccg gggataggct cagcactatc aataccaaca tcaattacgt ggacgttagc
     5041 gcccgcttgt gctgccagca cacacacgcc ggttgttccg cgggtcatat tttcagcctg
     5101 aatggctgtc acttcttttg gggaaatagc gaccccttcc tcccagacgc cgtgatcggc
     5161 acacataacc agtaccgctt ttttgcccac atgcggtatg ccattcaacc ccggcattcc
     5221 tgccagttgt atggcaagcg cctccagcct tccaaggcta ccaacaggtt tgagtaaccc
     5281 gtcaatatgc cgttgtgcac gcgacatagc ggcaggatcg atagcaggga tcgtattcag
     5341 taaatcggca agtgtttgca tgtcacgtct ctttatagca gtgccagcag gaagaccagt
     5401 tcaccaagct ctatagcagc tcccagcgta tcgccggttt gcccgcccag cgtacgtttg
     5461 agaagctgac cgaggatgaa aatcgccacc atcgtcacca ccatagcagc cacaccatgc
     5521 atacccggca ataatacggc agcaaaaata gcggccaagc cgagtgtgac gcaggtttgt
     5581 cgcccatcaa ttttgccaat gaatacattg ccaagccctt cttcccgggc ataacgatga
     5641 cgatacatca ataatgcggc agtcccgcgg ctgaccgcac atgctgccgc cagcgatgca
     5701 aggatcggtt cgccacgcag agccaactcg cttaacacca gaatctttgc cagtacaaca
     5761 aaaatcaatg ccagaccgcc gtgggtgcct aaacggctat cacgcatgat ttccaacatg
     5821 cgatcgcggc tacgtgcaga aaatacgcca tcacaggtat cggcgaggcc gtccaggtgg
     5881 aatcccccgg tcatcagcgc cagcacaagc acgctaaaca gtgccgccag tggtacgcca
     5941 caccatgcct gcagcaccat gaaaaccagc ccgctaatcg cgccaagcaa taatccaatt
     6001 aaaggaaaag taataatacc gcgagaataa tgctcgaaat ccagtccctg ggaccagcga
     6061 cgcggcacag gcagacgcgt aataaaagag agcatcgccc aaaataattt actcatttga
     6121 tttttactcc aatacccgaa accaccagcc acacttcatt cgccgcagcg gccagctgct
     6181 gatttacccg cccggcaata tcacgaaaat gacgtgccag acgactctcc ggcacaatcc
     6241 ccattcccac ttcgttagtc actaatacaa cctttgcggg gcaacgttgg caggcagcaa
     6301 tcaacgactg aatctcagca ttaatcgcct gttccatcgc ctgataatcc cattcatcag
     6361 ggtctttatc tccgccataa tcaaacaaca gattagtcac cattgtggta acgcattcca
     6421 gcaacacagc ttcatgcggg ttaatgtctg catgaattaa ttcatcaaga tgttgccagc
     6481 gctccaccgt gcgccagtgc gccgggcggc cttgccgatg atgttctatc cgtgcagcca
     6541 tctcatcatc aaggatttgc gaggtggcga tatacagaac ctgtgaagag tctccaataa
     6601 gcgcctctgc gtggcgactc ttcccgctcc gtgcgccccc cgtcaccaaa atcatcatac
     6661 cgactcctga ttcaattgta tgatggtatt cattgaacag aacaatggat cccccaccgt
     6721 tgctgaccgc aaaacattta cacgattaat actgcgcgta atataattaa aaatccagca
     6781 ttctcaatcc attacgaaag aagatggcat tgtggaatag ccagacttcc tcgtgttaac
     6841 gtccgatcat tggagtagtc accaaagcag atttagccag catggaagaa atatctttgg
     6901 tgaaagtttg gttacgggaa gctggatcgc acaacgtgtt agtaaccagt gcggttaata
     6961 acaatagagt tgcagaactt tttgccttgc ttcatacaga agatgtctgt cgctaatttc
     7021 gataggttat cagatgggaa tgatgactgt gatacacaga aaagaatcaa tgagcaatga
     7081 gggttgccgg gcaaccctca ttgaataaaa caggaattac ttcgcttcgc cgtttttcat
     7141 ttcgcccata gctttcagct ttttggagat atcgcggcgt tctttggaga gctcggcatt
     7201 tttgatgatg taatcgtcaa cgcgatcttc atagtcacct ttcatgctag cgatgatgcc
     7261 ctggattgct tcaacgctca tccccggctt gatgtaatca ctcaggttgt ccagcaacag
     7321 gacgcgcttc tggttgtcac ggatcttttt ctcaacgtcc tgaatttcac gttgcagttt
     7381 gttcttacga cggaacagac gaacaaattc cagtacgtcc tggaatgaag gcttggtagt
     7441 ttccattttt atacccctga taatgtgaga gtcggattcg tttaagcaac cgctattcgt
     7501 tagggccaac attactcata gctgccgcag atgacaatgc ttttatccct tttcactatc
     7561 atacccttta tccttgctga atcgaagcag cagcaagatg attctgaagt tcaggaatta
     7621 tttgcgcaag gcccggcaaa tcagattcga tagcaactca agctgatgcg ccgtttccat
     7681 gttcagccac acataaccat agattggtgt ttccacaacc ttcaggtcat ggtggttatt
     7741 gagcaactga cgcagctctt ccacagcatc gttcaatttt tccgtattgg caaaaaccgg
     7801 ctgcggatta ccttcgtaca gcgcatgaac aaggctcagc agtatttgct gcatcatgtg
     7861 ctgggtatca cgaagttttt gcgcgttcaa taacacgaaa tggctggggc gcgtggccca
     7921 gtatgcattg atttgcaact ccagcataca aaccagattg cggttaatgg tctggatacc
     7981 ttcatatatc gattttggaa tacgagtttc tttgctggcg ggcgcaatca gcccacgcat
     8041 tttcacggca tcggtcaaga gtttttgtag atggctttcc agacgtgggc gttcgagtaa
     8101 gttcggtgag aatgcagatt gatagacccg attatactcg gtcagacttt tcgccagttg
     8161 aatacgccag tggatgaacg cccgttgtgg ccagataccg gtaaacaaca ttgccagtaa
     8221 agagccgagg atcacatcgc cgcttcgcca taacgccgta tcaatttcac ctgtcgggga
     8281 acccacaaca attgccagcg tcaccccaat caataaacct tgatacggtt tcttgcccag
     8341 cgccagccaa ccgcaaagaa acatagccgc cgcgcaccag actaacatca gcggtaacga
     8401 gattaactcc agttgcagag cgataagacc taaaatcgaa cccaacaccg taccgccaat
     8461 acgttcgaag gcgcgaggga caacgttacc ccagaacgag attggcccca taatcaccac
     8521 catggtgacc agcggccagg tactttccgg gatagtaaac aggcggataa tgagaaaagt
     8581 gagcaggaat gccagcgcga cccgagtacc atgcacaatg cggtaatggc ggtatacccg
     8641 gatttcaaac gggcttaatg acttatcggc acgcacgcag aaactccgta atgaagatta
     8701 aaagcgctca taactcatct gtcggccaga aggggcgcct ctgaaatatc cgacggacaa
     8761 accgggaaca gtgtaatttc ccggcaggag cgaaaaaatt gaaaggcgca tcttactctt
     8821 ttcgctttca aaaaaaagag tggtcatcgc gttaacacac cgccctgaga tgaattagtg
     8881 atgttttggc tgcaccgcaa catacatttc gatatcccag tacccatctt ccgcgccatt
     8941 gttcaaataa acctcgaagc agggctttgg taacatttca taagcactgt cctgcaagag
     9001 gctattaaag aactgatacc agggtttagc aaaatcatca ccgactacac gagctaccgc
     9061 caccgcatac tgaccacctg aaatttctgt cagaatgacg ccctcactgt tttcgggaag
     9121 cgtaaagtaa cccggcaccg tcacgacggt gtcgcagcgt aatttttcgg cgggtgtttc
     9181 atctggattg tcgtaataga cagcaaccca ctccttcggc acaatatttt tgctatctac
     9241 ccacatcatc aactgctcaa agcctttctt taccgtctgt tcccacgggc caacgagatg
     9301 gaaacctgca acggtacgtt tatcttcctg cttaatctcg tagttcatga cgcctccatt
     9361 aatactgttt ttatatacag tatagttgca aaattaaaac cacaaggaat gagtgttgat
     9421 tatgcgagca gactcgcact cctgccagtc tgctgcaaaa gaaaggtcag gccttatggt
     9481 ggaaataatc actcaggcga gaaaacatgc tgccttcccc gacagattcc agggtaacca
     9541 gcggccagtg cgccacctgt ttatcacggt cgtaaagttc aatttcccct acccgctgat
     9601 gggcgctaat tggcgcggtg agctctttac catcaagggt atatttggct ttgatatgtg
     9661 gaatttcggc tttcggtagt accatccaga actcttgttc tgttcccagg gcgatatttt
     9721 ctttatcgcc ataccagatg cgttccgtac cgaccttttt cccacggtgc aaaatttgca
     9781 ccgtagtaaa gttttgttgc ccccaacgca gtaattttct tgcctcttcc tcacgacctt
     9841 ttgcgctgtc agcacccatt accactgcaa tgagacgacg ctgcccatct acagccgaag
     9901 caatgagatt aaacccggca ccagaagtat gacccgtttt caggccgtca atattcatgg
     9961 ttttatccca caataacccg ttacggtttt gctgggtgat accgttccag gtgaggcttt
    10021 tctcactgta catatgataa aactcgggct cgccgtggat gatagcgcga gaaagcacag
    10081 ctaaatcata agccgagcta tgctgacctg gcgcatccag gccatgcact gtttcaaaat
    10141 gcgtatcctt gagatgcagc ttctcggcat agttgttcat catttcaaca aactgccgtt
    10201 gcccaccggc aatatagtca gccagtgcaa cacaagcgtc atttccggaa tccacaatta
    10261 aaccacggct taaatcacgt accgatacgc gatcgccctc tttcaaaaac atcagtgaag
    10321 aaccgacaaa caccggatta tctttcgccc acgcatcacg ccccacggtg acaatatcgt
    10381 ctggcgtaat gcgatgacta tcgatagcgc gatccacgac ataacccgtc atcagctttg
    10441 tcaggctggc gggattgcgc tgttgatgct cattacccgc ggtgaggatc tgaccggtgg
    10501 tgtaatccat caatacccag gatccggcat gaatctcagg aggctgaggt gaaaaaggaa
    10561 tgttttccgc cgcaaaacca gacgataagt taaaaacgaa caaagaagca gcaataataa
    10621 gacggcgttt caacagcaaa ccctcaggag tttcaaatag ctgttctttt tacggaaata
    10681 cttatgaact ggctggaata aagtgcaaga aaatgtgact accctctcat ttttatctga
    10741 catgatctgt tgccactcgc tgccaaattg tggcgctaaa gctgattagc acggtgatat
    10801 ttgatactct ggcagacagt agaaataacg gatttaacct aatgatgaat gacggtaagc
    10861 aacaatctac ctttttgttt cacgattacg aaacctttgg cacgcacccc gcgttagatc
    10921 gccctgcaca gttcgcagcc attcgcaccg atagcgaatt caatgtcatc ggcgaacccg
    10981 aagtctttta ctgcaagcct gcggatgact atttacccca gcctggagcc gtattaatta
    11041 ccggtattac cccgcaggaa gcacgggcga aaggagaaaa cgaagccgcg tttgccgccc
    11101 gtattcactc gctttttacc gtaccgaaga cctgtattct gggctacaac aatgtgcgtt
    11161 tcgacgacga agtcacacgc aacatttttt atcgtaattt ctacgatcct tacgcctgga
    11221 gctggcagca tgataactcg cgctgggatt tactggatgt tatgcgtgcc tgttatgccc
    11281 tgcgcccgga aggaataaac tggcctgaaa atgatgacgg tctaccgagc tttcgccttg
    11341 agcatttaac caaagcgaat ggtattgaac atagcaacgc ccacgatgcg atggctgatg
    11401 tgtacgccac tattgcgatg gcgaaactgg taaaaacgcg tcagccacgt ctgtttgaat
    11461 atctctttac ccatcgtaat aaacacaaac tgatggcgtt gattgatgtt ccgcagatga
    11521 aacccctggt gcacgtttcc ggaatgtttg gggcatggcg cggcaatacc agctgggtgg
    11581 caccgctggc gtggcatcct gaaaatcgca atgccgtaat tatggtggat ttggcaggag
    11641 acatttcgcc attactggaa ctggatagcg acacattacg cgagcgttta tataccgcaa
    11701 aaaccgatct tggcgataac gccgccgttc cggttaagct ggtgcatatc aataaatgtc
    11761 cggtgctggc ccaggcgaat acgctacgcc cggaagatgc cgaccgactg ggaattaatc
    11821 gtcagcattg cctcgataac ctgaaaattc tgcgtgaaaa tccgcaagtg cgcgaaaaag
    11881 tggtggcgat attcgcggaa gccgaaccgt ttacgccttc agataacgtg gatacacagc
    11941 tttataacgg ctttttcagt gacgccgatc gtgcagcaat gaaaattgtg ctggaaaccg
    12001 agccgcgtaa tttaccggca ctggatatca cttttgttga taaacggatt gaaaagctgt
    12061 tgttcaatta tcgggcacgc aacttcccag ggacgctgga ttatgccgag cagcaacgtt
    12121 ggctggagca ccgccgccag gtattcacac ccgagttttt acaaggctat gcagaagaga
    12181 ttcagatgct ggcgcagcag tatgccgacg ataaagaaaa agtggcgctg ttaaaagcgc
    12241 tttggcagta cgcggaagag atcgtctagt ttgaagcctc cgccgctgat acggcggagg
    12301 acaatgttgg gtcaggcttt ttgaacggtg atgctccacg cagcatcgcc aatctgctga
    12361 taatcggtga tggcatgtcc ttcttctgcc gcccattgtg ggattgcttc tgttgcctgg
    12421 gtgcaatcaa actcaattac caactcatcg ccgctggcca tttctgccag tgctgctttt
    12481 gcttctatga gtggaaacgg acagacctgc gtcaccacat ccagcttttt aatcgccatc
    12541 tttacacctt acttaatttg ccgcagcagt tgccagtcgc gccttacgct gcggtcgaac
    12601 atacacaagc caggacgcag tccagactcc gagaatcata aatacaaggc caatccagcc
    12661 ctgccaggtc atcattgcag tcataaccag tccattaccg atagagcaac cacctgcaat
    12721 actggcaccg aagcccatca gtacaccacc taacccgcta cgtactgttg tttgtgcatc
    12781 agctgcgcga acgcggaact cacggctcgc tttggctgca ataaatgacc ccacgaagat
    12841 ccctaacacc aggaaaacgc cccagttaat gtatttcacg tcacccgcga ccagaaattg
    12901 cagaatattg gccgttgggg aagtgatgcc taacccaaac atacgtccgg ttgcttcact
    12961 caggggccag gctaaaagcg caatcaaacc gatgagtaca gcagtgacaa agggatgcca
    13021 gcgtttttca aacagaatat gagcgatccc agttcggcgc ggcggtaagg tcgcgacttt
    13081 gagttttggc ttcttcaact ctttcatcac cacccagagc gttatcacca gcaatacggc
    13141 aaccaacggc cacacagaca aattaaaagt ctcagcaata gagttatgtt cagtactgta
    13201 gtgctgcaag gtttgattta aaccactggc gtgtggagaa cgcatcaccg cactcatcac
    13261 catataagtg aaaagcgcga tccaactgcc gatcaatccc tcacccgcgc gataccaggt
    13321 cccggtggca catccacccg ccagaacaat tcccagcccg aagatatacc cacctataac
    13381 agtaccaagc cacgggaacg ccccggcttc gtaagtcagt aggcctgcct gaattaacgc
    13441 aaaaaccccc acgctttgta tcgaaattgc aatcagcaag gcgtaaaaca tgcgattatt
    13501 tttcgcgata tacatatcgc gaaaaccacc agtcaggcaa aaacgcccac gctgcatgac
    13561 aaatcccagc agagcaccac aaattagccc gcttaatatc attgaaaaca taaattaatt
    13621 aaccagatga atgtta
//
LOCUS       DAPGOF010000129        13371 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.130, whole genome shotgun sequence.
ACCESSION   DAPGOF010000129 DAPGOF010000000
VERSION     DAPGOF010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.130"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..323)
                     /locus_tag="RHV13_003387"
     CDS             complement(<1..323)
                     /locus_tag="RHV13_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242890.1"
                     /translation="MAKVDQTKANALLRAARTMSLGANKNTIRILYGNHVAENIDFEP
                     GAKTKLKKGIRGWSKRTDNRRAFSAVIDVAVNYFGFDPINPEASFRQIDFNRIMSVLH
                     NLGYFV"
     gene            complement(325..1125)
                     /locus_tag="RHV13_003388"
     CDS             complement(325..1125)
                     /locus_tag="RHV13_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trhR"
                     /protein_id="HDV1242891.1"
                     /translation="MSESDNLTRSLLGKLFRTEISLIRSYRAFLMLLPVHGSSAYQTG
                     TPLLQRRLFGKGFSTAADLAFEVETRPGSFLVPRTLGKEITWERFFSAVLDGDSNVIR
                     EYDTNDIDYGIYDAGEKVTFLNGTVDIYNPKKIHELRSKCVDIQNDYFMQVFFISMLA
                     PEFVSVFFGLKPTTADAIKDIGYSSLKTINDVVLFPRTIAFSQGHIEESVSLKTKVFA
                     WAYELSADIRLGKVSDDLMELLRYDTMFTSHRQDVFNTLTNKVMLKDY"
     gene            1884..2369
                     /locus_tag="RHV13_003389"
     CDS             1884..2369
                     /locus_tag="RHV13_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer protein"
                     /protein_id="HDV1242892.1"
                     /translation="MSNSNLISSIELTRKNLRAQIEGLRVEAEDLITKYWIKWKERNH
                     QEINLTRHSKVPKREYLGSYAPKVELIGNARKVTITWHQFSPYKTRAPSHMSKRVQPM
                     KSGKYSKNCFVNHASWEYEMISETEALLEPYREMLELYHSAYIELGRKIRQYSKTKVA
                     Q"
     gene            2366..3103
                     /locus_tag="RHV13_003390"
     CDS             2366..3103
                     /locus_tag="RHV13_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000128631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242893.1"
                     /translation="MTDAIITDNERINIEPKDVMVKGSNKKQGVNAQTSTQRRPEHQG
                     MAKVIINPGTPDFNRFLTARNGAVIRGFDDVSIAISSLFKTVDAVKHPDLVQAIQDWF
                     NELHEENNKMKENLVAYIKSIEFDKNDSFMSSTQFVPFSFEPVQLNFNNHNTMRFYKY
                     IFEMNQLMNTMYEYNSLGLLAVSDYPVMSHNIIKSINLYVENVKKTLNVSRRKDGPYS
                     PAEFITKVMQYKSVQAYIAAELSGKRR"
     gene            3113..6259
                     /locus_tag="RHV13_003391"
     CDS             3113..6259
                     /locus_tag="RHV13_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HDV1242894.1"
                     /translation="MNFRALYLCIKRILGIFSSQENDATSVMIEDISSLSPFAQILGD
                     QKYTVPDHPNPEVLKFIEYPTRPTGIQTFNEQSILSLYREKLHSISMMLAISDSDIRD
                     DAYTFTNLVLKPLVEYVRWIHLLPASENHHHNGIGGLLSHSLEVAILSLKNAHHSELR
                     PIGYQDEEVVRRKVYLYAAFICGLVHDAGKVYDLDIVSLNLASPIIWTPSSQSLLDWA
                     RENDVVEYEIHWRKRIHNQHNIWSSVFLERILNPVCLAFLDRVNKERVYSKMITALNV
                     YTDGNDFLSKCVRTADFYSTGTDLNVLRDPIMGLRSNDAAARAISTIKHNFTSININN
                     YNAKPMHIIIVNGEVYLNENAFLDFVLNDFELHKYNFPQGEAGKTVLVESLVQRGYVE
                     PYDDERVVHYFIPGIYSENEISNIFRNGIGKLEFYNLLKLRWIGLIFDSYKIPDSVPG
                     LFSVNANKDFIYIDEQKTVTEYRRPVPGRDAITKITDTVETAVLKVNDLGRSTASIDV
                     DIHSKKNEGSSDDFEKKAESDNEIDNDTQIVKSEGEEAADPVIPDIEESEDESAKDTE
                     SHVLVNQLHELLLSAPLSNDYIVCVDAVPYLNIDATMALLPGLDEKAFSEEPYFQLTF
                     REGSLDGMWIVRDIDDLRLVQLGDNCAGFQLTYHEPRRPTTLKSLFNTSMYQALVIND
                     ESSVENSAPRPKQTLELPPPRVNAVEEHSGDVEYHGTDSASATGPLKTEAVEYEHYQH
                     LFEEEDEEHEIIDYTDFTQLSVSRPEVGSCATSSSVHNEKLLPEPSELPELNREQNAD
                     PQGTIERSMDVSVGQENSEPDTEGNCPPPAEVVYSQTEAAATSVMASEEPALPPVLEE
                     SNGEHAPTDAKGHHLSPALARLFAPTAPVEKQNPKRNRNKSSDKAEVQKPASPVSGHN
                     LNSKVFATTESDQNGEFSLISEGDVTELEFVEIASVLHQILSKMEVAFKRKRKNRFMV
                     STPNTLYLTQSCVEKFGSQLEAQDLFNKLPQYLVNSGAVINTKCHAFNMPTLLAASDR
                     AKVDIERIINNLKEAGNL"
     gene            6259..8343
                     /gene="traD"
                     /locus_tag="RHV13_003392"
     CDS             6259..8343
                     /gene="traD"
                     /locus_tag="RHV13_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284076.1"
                     /note="Members of this protein family are the putative
                     conjugative coupling factor, TraD, as the term is used for
                     the SXT and TOL plasmid systems; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer system coupling protein
                     TraD"
                     /protein_id="HDV1242895.1"
                     /translation="MSDSKRTNLHAQENFYRPILEYRSASILLICSVSMLYMGLSSDG
                     LDIAPIVLFTSILLFLLCLYRCKTAAPFLMAHWRVFKRHFMFVSLDSLRVINKSNFFS
                     NERKYRQLVQDYQNKNKDIPERKSYFCDGFEWGPEHADRAYQIANLSSDKREIELPFV
                     FNPIKRHFDAMARKMGGSNAIFAVERREPIFVTEDNWFGHTLITGNVGTGKTVLQRLL
                     SISMLHLGHVVVVIDPKNDAEWRESLMEEAKTLGLPFYKFHPGQPASSVCIDVCNTYT
                     NVSDLTSRLLSLVTVPGEVNPFVQYAKALVSNVISGLSYIEKKPSIYLIHKNMKSHMS
                     IVNLTVKVMESCYARYYGYDVWTEKVKYVANETLPVRFKRLAEWFTAHFMNYEGSEQI
                     DWLDTVSQLIDYSMSDPEHMAKMTAGIMPVFDMLIEKPLNELLSPNPNSVSSREIVTS
                     EGMFSTGGVLYISLDGLSNPDTAAAISQLIMSDLTSCAGSRYNAQDGDMSANSRISIF
                     VDEAHSAINNPMINLLAQGRAAKIALFICTQTISDFIAAASVETANRITGLCNNYISL
                     RVNDTPTQTLVVENFGKSAISTNMVTYTTGSETSLPHNNFSGSISERKQTTLEESIPK
                     DLLGQVPMFHIVARLQDGRKVVGQIPIAVAEKQMKPNTTLSEMLFKKAGKVTLRQNLD
                     IKNLNKFLRKLH"
     gene            8343..9452
                     /locus_tag="RHV13_003393"
     CDS             8343..9452
                     /locus_tag="RHV13_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242896.1"
                     /translation="MDEKIVLTRQQILSSALKVSKCRSLVKRRFQSLGLKYADSQEVR
                     DRLTKIEKNAFHHLGKFCQSNDIDSLFSMANTLSELFLLKGELITTDPFGDRETSYWS
                     VPQGSCHEWIQSLTTSEGPERKFVSFRISFDNNDERCDLVKKNARMLGCYLLPYFVDL
                     TRTVGAFINLPGSVSFKQVQRIKPQIHPETTHSHIVTIEDSPFLSRLKFKIITAIDRL
                     PDPNGLYTNTFNSIIDRALLTHLKTEQEKIDSPRVCKNVISAFADSTLSLPVFNIGLN
                     EQYRYWTPWGINFIEFSRQAAKARTAVFVPDVGQIEWKSAEHKELAELSLIDQIIPKQ
                     YHWLLGIPTMWRNNYCNHDQRLALFREWRESNGCG"
     gene            9439..10101
                     /locus_tag="RHV13_003394"
     CDS             9439..10101
                     /locus_tag="RHV13_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4400 domain-containing protein"
                     /protein_id="HDV1242897.1"
                     /translation="MAADNTSRAAVLRTMLFFGMVIYFGYSLAFQNTEELKYQITQEV
                     NASRSIISNDRWKSVIANSEATLNWLVHDYKLIDYLNTILIPDTKKPARGINIVAEKF
                     TSINYTMAKNIPLLLYQSIFRWNLILGWLIVFLPYLFAMLADGMYQWKLKRYVFGKVT
                     VQFYRIWFRAFWVISALTMVYLVMPNMSLFNNIAQLFPPVALLILGIALNRLWSNFQK
                     LM"
     gene            10112..11293
                     /locus_tag="RHV13_003395"
     CDS             10112..11293
                     /locus_tag="RHV13_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HDV1242898.1"
                     /translation="MLSSKKRKSPTNIKESLNDNADRFYKMFRIHTTAKVAMSLIAMT
                     AVGFSFYNLYEQWQDAEGKKDHIAVIRISGEMGTGSETGDGTVIATALAKAYNNPHAK
                     AVIIEAESGGGGPSDAIIIYRQINALKNHQPQIERVSDAGCSLSSVAADKSNKTGSTE
                     RGDEARSKQNSLEVLSSGTGRFFSDIADSYKPIIVSVKGICASACYYAVSPADAIYAD
                     SNALIGSIGVRMDHWNLSEIMSTVGVKNEPLTAGEFKDALDPFHPLSDSTREFMQKEI
                     LNTMHEKFITDVELGRGKKLLSRHDADAVSLYSGRVWPTPQAVKYGLVDGDLTSVEIR
                     TRLSKMYSTDTFKNYNEPHRNLRSALGMLMSLSSNIESLTGTTTRLVESVNATSYPSV
                     R"
     gene            11295..12026
                     /locus_tag="RHV13_003396"
     CDS             11295..12026
                     /locus_tag="RHV13_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HDV1242899.1"
                     /translation="MDAFNLLWSITGVAFIILIFVVLLCLLGFMTSAIAERRTAKAIE
                     SGLPEEAQGLLSDLTFQLSAHSTTQVDHILVAPHGIYVIEQKNYVGKLYGTLEESHWR
                     KWTQSRTLKLQNPFKQNQGHIRAIQSALKARELECINVVIINGRCKFDGIKPEWLCMG
                     MDDFIHKVKQRRGLRLFTPESVQHICSVLKSTRKSPGLYTDLTHIHNITTKYKAPMKF
                     EQRVTYILLNFIHYLWASLFTKQKP"
     gene            complement(12313..12666)
                     /locus_tag="RHV13_003397"
     CDS             complement(12313..12666)
                     /locus_tag="RHV13_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242900.1"
                     /translation="MTTNTTTTTYRSEEIVPFRRPKGDLDSRYMPQVYAMVRNWASNP
                     AQYGEGVLASYRQPAVNLAYQVKGTRVVLILVPVECEPPGVIMTETVLWPSLSLVEVL
                     QTLQEAWQNIPALNP"
     gene            complement(12732..13016)
                     /locus_tag="RHV13_003398"
     CDS             complement(12732..13016)
                     /locus_tag="RHV13_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242901.1"
                     /translation="MNKFLYVLLAWIVVLFIANKLLARRKPKTVKILVQRNGKAAEVD
                     AVVVQGSKRANNSSVADSDAVDSYFEMNPYSRENQSNGWAMLARDDDQLK"
BASE COUNT         3903 a         2900 c         2826 g         3742 t
ORIGIN      
        1 acaaagtatc ccagattatg aaggacactc attattcggt tgaagtcaat ttgccgaaaa
       61 ctcgcttccg gatttattgg atcaaaacca aaatagttaa ctgcgacatc aataacagca
      121 gaaaaggctc ggcggttatc tgtgcgtttg ctccagcccc ggataccttt tttgagtttt
      181 gtttttgcac caggctcaaa atcgatattt tcagcaacat gattaccgta caaaattcgt
      241 attgtatttt tatttgcccc aagcgacata gtccttgctg ctcgcaatag agcattagct
      301 tttgtttgat cgacctttgc catattaata atcctttaac ataactttat ttgttaaagt
      361 gttgaacaca tcctgacggt gactggtgaa catagtgtca taacgtaata gttccatcaa
      421 atcatcacta acttttccta accggatgtc agcagacaat tcataagccc aggcaaaaac
      481 tttcgtttta agtgaaacac tctcttcaat atgcccttgt gagaaagcaa ttgtacgtgg
      541 gaaaagaacg acatcgttaa ttgtttttaa cgatgaataa cctatgtctt taatagcatc
      601 agctgtagtt ggttttaaac caaagaaaac acttacaaac tctggtgcga gcattgagat
      661 aaagaacacc tgcataaagt agtcattctg tatatcaaca catttagaac gcaactcatg
      721 aatcttcttg ggattataga tatccactgt accattcaga aaagtcactt tttcaccggc
      781 atcgtaaata ccatagtcaa tatcatttgt atcatattca cgaattacat tggaatcacc
      841 gtccaagacc gcagaaaaaa acctctccca tgtaatttcc tttccgagtg tacgcggaac
      901 caaaaaacta cctggacgtg tttccacttc aaaagctaaa tcagcggctg tactaaatcc
      961 tttcccaaat aggcgacgct ggagaagagg agtcccagtt tgatatgcag aagatccatg
     1021 cacaggcaat aacattaaaa atgcacggta actacgaata agcgaaatct cagtgcggaa
     1081 aagtttaccg agtaagctac gagttaaatt atcagattct gacatgtgct gattgtcctt
     1141 gcgccattac aattgttcaa atcttcaaca tggatttttt gttaggtgtt cggtagggtg
     1201 aaaagtaact tttagtattt tcttaaggct tgaataagcc ttaaatctat cccttcaacc
     1261 acaccgctta cttgtaacac tttttaacca aactgaccag aattttatca aaatttcttc
     1321 gcatgctaac gaaatgtgaa gcgattaaaa aaaaacctaa atccagcccc tcccaacgtt
     1381 ggattagaat aacgacgtct ttcactgacc gtcataaact gacattttta cttttcatca
     1441 acctcatgct tacataggat cactaaacaa agaaaagtga ccctatgaat gcctcacata
     1501 gggtcactta tgtttgaaaa gtgaccctat gtaatgtcgt catagggtca ctttttataa
     1561 aagtgtcact atggacaaag ttccataggg tcattttttt tggaaaagtg gcactagata
     1621 tatgtataac tttaatagag caatgcacat tatcaaaagc aataatccac aatggtcatt
     1681 ccctcaaaag taactttaga gggtagcgat ggaagtgagg ctaaactcgc tgttactgtt
     1741 tctgaaggcc cctcccgaat gggggaaaca gcgagtgcat tacatctgtt caattccctc
     1801 atcaaagagc attcaaaaat cttctgttcg tgaaaaaaaa cggaaatttc ctccggtata
     1861 gtaatttcac atcaggaggc gtcatgagta attcgaatct tataagttct atagagctaa
     1921 cacgtaaaaa tttacgtgct cagattgaag gtttgcgtgt tgaggctgaa gatctcatta
     1981 caaaatactg gatcaagtgg aaggaacgaa accaccaaga gattaacctt acccgacata
     2041 gcaaagttcc aaagagggag tatctgggtt cttacgcacc aaaagttgag ctaattggta
     2101 atgccagaaa agtaacgatc acttggcatc agttcagccc ttataaaaca agagcgccta
     2161 gccacatgtc aaaacgggtg caaccaatga aaagtggaaa atattctaag aactgttttg
     2221 taaaccatgc cagctgggaa tacgaaatga tttctgaaac ggaagcattg ctggagcctt
     2281 acagagaaat gcttgagctc tatcattcag catatatcga acttggacga aaaatccgtc
     2341 aatactcaaa aactaaggtg gcacaatgac cgacgcaatt atcacggaca atgagcgtat
     2401 taacattgaa ccaaaagatg taatggtaaa aggttcaaat aaaaagcaag gcgtaaacgc
     2461 tcaaacttct actcaacgta gaccagagca ccagggtatg gccaaagtta ttattaaccc
     2521 cggcacccca gattttaacc ggtttttaac tgccagaaat ggagcagtca tcagaggttt
     2581 tgatgatgtg agtatcgcta tttcctctct ctttaaaacg gttgatgcag ttaaacatcc
     2641 tgaccttgtt caggctattc aggattggtt caacgagctg catgaagaaa acaataagat
     2701 gaaagaaaat cttgttgctt atattaagtc aattgagttc gacaagaatg actcattcat
     2761 gtcatcaact cagtttgtac ctttcagttt tgaaccagta caactcaact tcaataacca
     2821 caacaccatg aggttttaca agtacatctt cgagatgaac cagctcatga acacaatgta
     2881 tgagtacaac tcattgggtt tactggctgt aagcgactat ccggttatgt ctcacaacat
     2941 tataaagagt attaatttat atgttgagaa tgtgaaaaag actctgaatg tttctcgccg
     3001 taaggatggg ccatacagtc cagcagagtt catcaccaaa gtaatgcaat ataaaagtgt
     3061 gcaggcatac attgcagccg aactgtcagg caaacgtcga taaggtatga tgatgaattt
     3121 cagggcgctg tatttatgta ttaaacggat tttggggata ttctcatctc aggagaatga
     3181 tgcaacctct gtaatgattg aggatatatc aagcctctct ccttttgcgc agattctagg
     3241 agatcagaag tacactgttc ctgatcatcc aaatccagaa gtcctgaaat tcatcgagta
     3301 tccaactcgt ccgacgggca tacagacatt taatgaacag tcaatcctgt ctctgtatcg
     3361 ggaaaagctg cactcaattt caatgatgtt agctatcagc gatagcgaca tcagggacga
     3421 tgcatataca tttactaatt tagtattaaa gcccttggtt gaatatgttc gctggataca
     3481 tcttttgcca gcttccgaaa atcatcatca taatggtatt ggtgggttac tttctcacag
     3541 cctggaagtg gccatactct ctttaaaaaa tgcgcatcac tcagaactga gaccaatcgg
     3601 atatcaagat gaagaagtag tccgtagaaa agtatatctc tatgctgcgt ttatctgtgg
     3661 tttagtccat gatgccggaa aggtttacga tctcgatatt gtaagcctga atttagctag
     3721 tccgatcatt tggacgccaa gctcacaaag tcttcttgat tgggcacgtg aaaatgacgt
     3781 ggttgaatac gaaatccact ggcgaaagcg tattcataat caacataata tctggtccag
     3841 cgttttcctt gagcgaatcc taaacccggt atgtcttgca tttttggatc gggtaaataa
     3901 agaacgtgtt tattcaaaaa tgatcaccgc cctaaacgtt tatactgatg ggaatgactt
     3961 tttgtctaaa tgcgtgagga ccgctgattt ctattctact ggtacagacc ttaatgtttt
     4021 acgtgaccct atcatgggtc tgcgttctaa tgatgcagca gcaagggcca ttagtaccat
     4081 caagcacaac tttaccagta tcaatatcaa caattacaat gctaagccta tgcacatcat
     4141 tatcgtaaat ggtgaggtat accttaacga gaacgcattc cttgattttg ttctcaatga
     4201 tttcgaatta cataaatata acttccctca gggagaggct ggtaagacgg tattggtaga
     4261 atccctagtt caacgtggtt acgtcgagcc ctatgatgat gagcgtgtcg ttcactactt
     4321 cattccgggt atctattcgg aaaacgaaat atcaaacatc tttagaaatg ggatagggaa
     4381 actagaattc tataaccttc ttaagctaag gtggattggt ttaatctttg attcctataa
     4441 aatccctgac tcagtgccgg gtctttttag cgttaatgcc aataaagact ttatttacat
     4501 tgacgagcaa aaaacagtta ctgaatacag aagaccagtg cccggtcggg acgctataac
     4561 taaaattact gatactgttg agacggctgt tcttaaggtt aatgatttag gccgctccac
     4621 tgcctctatt gatgttgata tacattctaa gaaaaatgaa ggctcatccg acgattttga
     4681 gaagaaagcc gaaagtgata atgaaattga taatgacacg cagatagtta aatctgaggg
     4741 tgaggaagca gctgatcctg tcattcctga tatagaagaa agtgaagatg aatcggcaaa
     4801 ggacacggaa tcccatgtcc tggtcaacca acttcatgaa ctccttttaa gcgctccact
     4861 ttccaatgac tatatagtgt gtgtagatgc tgtcccctac ctcaacatag atgccactat
     4921 ggcattgctg ccgggattag atgaaaaagc atttagtgaa gagccctatt tccaactgac
     4981 attcagagaa ggctctctcg atggaatgtg gatagtaagg gacattgacg atctgcgttt
     5041 agtccagctc ggcgataact gcgccgggtt ccagctcacc tatcatgagc caagaaggcc
     5101 aacaacttta aagtcacttt tcaatacttc tatgtatcag gcattagtga ttaatgatga
     5161 gtcttctgtt gaaaactctg ccccacgtcc aaagcaaacg cttgagctgc ctcccccacg
     5221 tgtaaatgca gtcgaagaac attctggcga cgtggaatat catggaaccg atagtgcatc
     5281 cgcgactggt ccgctgaaaa ctgaggctgt ggaatatgaa cattatcaac atctttttga
     5341 ggaagaagat gaagagcatg agatcattga ttataccgat ttcacacagc ttagtgtttc
     5401 tcggccagaa gtcggtagtt gtgcaacaag ctcttcggtc cataatgaaa agcttctccc
     5461 cgagccgtca gaattaccag aacttaaccg agaacagaac gctgatcccc agggtacgat
     5521 tgaacgctct atggatgttt ctgtaggaca agaaaactca gaaccagata ctgagggtaa
     5581 ctgcccacca ccagctgaag ttgtgtattc ccagaccgaa gcagccgcaa ccagtgtgat
     5641 ggcaagtgaa gagcccgcct tacctcctgt acttgaagaa tcaaatggag agcatgcgcc
     5701 cacagacgct aaggggcatc atctttcgcc agcactggcc agactgttcg ccccaacagc
     5761 accggtagaa aaacaaaatc ccaagcgcaa ccggaataaa tcatcggaca aagcagaagt
     5821 acaaaaacca gcatcacctg tttctggtca caatctgaac agtaaagtct ttgcaactac
     5881 tgaatcagac caaaatggtg aattctcgct tataagcgaa ggtgacgtta cagagcttga
     5941 atttgtagaa attgcgtcag tactgcatca aattctgtcc aaaatggagg tggcgtttaa
     6001 aagaaagcgc aaaaacagat ttatggtaag tactcctaat actctctatc tgacccaatc
     6061 ttgcgtggaa aaatttgggt cgcagttaga ggctcaagat cttttcaata agcttcctca
     6121 gtacctcgtt aattctggtg ctgtgatcaa tacaaaatgc catgcattta acatgcctac
     6181 tttgctggcc gcatcagacc gggcgaaagt ggacattgaa cgaattatca ataacctgaa
     6241 agaggctggg aacctataat gtcagattcg aagaggacta acctccatgc acaagagaat
     6301 ttttatcggc ctattcttga gtatcgtagt gcctcaatac ttttgatttg ttcggtttct
     6361 atgctttata tgggactttc ttcagacgga ctggacatcg ccccaatagt tctgttcacc
     6421 tcaatattgc tttttttgct gtgcctttat cgttgcaaaa cagcagcgcc attcttgatg
     6481 gctcattggc gtgtattcaa gcgacatttc atgttcgtta gtcttgattc actgcgtgta
     6541 attaacaaaa gtaacttttt ctccaatgag cgaaagtatc gtcaactggt tcaggattat
     6601 caaaataaaa ataaggatat acccgagcga aagtcctatt tttgtgatgg gtttgagtgg
     6661 ggcccggaac atgcggatcg ggcatatcaa atagctaacc tctccagtga taaacgtgag
     6721 atcgagttgc cattcgtctt caacccaatt aagagacatt ttgacgcaat ggctcgtaag
     6781 atgggcggca gcaatgcaat ttttgctgtg gaacgtaggg aacccatatt tgtaactgaa
     6841 gacaattggt ttggtcatac tctgataacc ggaaacgtgg gtaccgggaa aacggtgcta
     6901 cagcgtttac taagcattag catgcttcat cttggtcatg ttgttgttgt catagatcca
     6961 aagaacgatg cagagtggcg agaatctcta atggaagagg ctaagaccct cggtcttcct
     7021 ttctataaat ttcatcctgg ccagccagca tcttcggtct gtattgatgt ctgcaacacc
     7081 tatactaatg tttctgacct aacatctcgt cttttgagct tggtgacagt tcccggtgag
     7141 gttaacccat tcgttcagta tgccaaggct ctcgtttcca atgtgatttc cgggctttct
     7201 tacatagaga aaaagccttc tatttatctg attcacaaga acatgaaaag ccacatgtct
     7261 attgtgaatc ttacagtcaa ggtaatggag tcatgttacg cgagatatta tggttacgat
     7321 gtctggaccg aaaaagttaa gtacgtcgct aacgaaacct tgccggtacg tttcaagcgt
     7381 ctcgctgaat ggtttacggc tcatttcatg aattacgagg ggtccgaaca aatagactgg
     7441 ttagatactg tttcccagct tattgattac tcaatgtccg acccagagca tatggctaaa
     7501 atgaccgcag gtattatgcc tgttttcgat atgctcattg aaaaaccttt aaatgagctt
     7561 ttatctccta acccgaactc agtatcatca agagaaattg taacaagcga agggatgttc
     7621 agcactggcg gggtgctata catttcgctt gatggccttt caaaccccga tacagcagct
     7681 gctatttctc aattaataat gtcagatctg acctcctgtg ccggtagtcg ctacaacgct
     7741 caggatggtg atatgtcagc taattccagg ataagtattt ttgttgatga ggcacactca
     7801 gccattaaca accctatgat taaccttctg gcgcagggtc gtgcagcgaa aattgcactg
     7861 tttatttgca cccaaaccat ctccgacttt attgctgcgg caagtgttga aactgccaat
     7921 cgaattacgg ggctctgcaa caattatata agcttgcgag tcaacgatac tcctactcag
     7981 acgcttgttg tagagaactt tggtaagagt gcgataagca caaatatggt cacttatacg
     8041 accggttcag aaacatcttt gccgcataat aacttctcag gttcaatctc agaaagaaag
     8101 cagacaactt tagaagagag tattccaaag gatctcttgg gtcaggtccc tatgtttcac
     8161 atagttgcca ggcttcaaga tggcaggaag gttgtaggcc aaatccctat tgctgttgct
     8221 gagaaacaaa tgaagccaaa cacaactctg tcggaaatgc ttttcaagaa ggccggaaaa
     8281 gttactttgc gtcaaaatct cgatatcaaa aatcttaata aatttctaag gaagttgcat
     8341 tgatggatga aaaaattgtc ctgacgcgcc agcaaatttt aagctctgca ctaaaagtca
     8401 gcaaatgtcg ctcgctcgta aaaaggcgct ttcagtcttt gggtttaaag tacgcagatt
     8461 ctcaggaagt tcgagatcgg ctaactaaga tagaaaaaaa cgcgtttcat catttgggaa
     8521 aattttgtca gagcaacgat atcgattccc tattctccat ggcaaatacc ctgtctgagc
     8581 ttttcctttt aaagggagag ttgatcacga ctgatccctt tggggaccgt gaaaccagct
     8641 actggtcggt accacaaggt tcatgccatg aatggattca atcgctgacc accagcgaag
     8701 gtcctgaacg taaattcgtt agtttccgta tttctttcga taacaatgac gaacggtgtg
     8761 atcttgtaaa gaagaatgcc cgtatgcttg gttgttatct tttgccttat ttcgtcgatt
     8821 tgacacgcac ggtgggcgca ttcattaatt tgccaggtag tgtctctttt aaacaagtac
     8881 agcgcatcaa accccagatt catccagaaa caacacatag tcatatagtt actattgagg
     8941 actctccctt tctttctcgg ttaaaattta aaatcattac tgccatagat cggcttcctg
     9001 accctaatgg cctttataca aacaccttta acagcattat tgaccgtgca ctgctgactc
     9061 atctcaaaac cgaacaggaa aaaatagata gtccaagagt ctgcaaaaat gtgatttcgg
     9121 cttttgccga ctcaactctt tctctgccgg tgtttaacat cggcctaaac gagcagtaca
     9181 gatactggac gccgtggggc atcaacttta tagaattttc ccgccaggcc gcaaaagcaa
     9241 ggaccgctgt atttgttcct gatgtgggac agatcgagtg gaaaagcgca gagcataaag
     9301 aactggcgga gttaagcctc atcgaccaaa tcatacctaa acagtaccac tggctcctgg
     9361 gtatcccgac gatgtggcgt aacaactatt gcaatcacga tcagaggcta gctctttttc
     9421 gtgaatggag ggaaagcaat ggctgcggat aatacaagca gagcagcagt gctcagaaca
     9481 atgctttttt ttggcatggt catttatttt ggttacagcc tggctttcca gaatactgag
     9541 gagctcaaat atcagattac tcaggaggtt aatgcaagcc ggtccatcat atcaaatgac
     9601 cgttggaagt ctgttattgc aaatagtgaa gcgactttaa attggttggt acatgactat
     9661 aagttaattg actatctgaa tactattctg atccctgaca ccaaaaaacc agccagaggt
     9721 attaacattg ttgctgaaaa atttacctct attaattaca ctatggccaa aaacataccc
     9781 ctcttacttt atcagtccat tttccggtgg aacttaatcc tggggtggct aatcgttttt
     9841 ctgccctatc tatttgccat gctagcagat ggaatgtacc agtggaaatt gaagaggtac
     9901 gtatttggta aggttacagt tcagttttat cgtatttggt ttcgagcatt ttgggtgatc
     9961 agtgctttaa cgatggtcta cctggtcatg ccgaatatgt cactatttaa caatatcgct
    10021 caacttttcc caccagtcgc tttattgata ctgggaattg cattgaatcg cttgtggtct
    10081 aactttcaaa aactcatgta aaggtggtca gatgttaagc agtaaaaaaa ggaagtcccc
    10141 tacaaatatc aaagaatcgc tcaatgataa cgctgaccgt ttttataaga tgtttcgcat
    10201 tcataccacg gcaaaagttg ctatgtcgct aattgccatg accgctgtag ggttttcttt
    10261 ctacaatctt tacgaacaat ggcaggacgc tgaagggaag aaagaccata tagctgtaat
    10321 acggatttct ggcgagatgg gtaccggctc ggaaacgggc gatggaacag tgatcgcaac
    10381 agctcttgcc aaagcttaca ataatcccca tgccaaagca gttattatcg aggcagagtc
    10441 aggtggtggt ggtccctctg acgccatcat tatttaccgc cagataaacg cgcttaaaaa
    10501 ccaccagcca cagattgaac gcgtatcaga tgccggttgc tctctttcat ctgtagccgc
    10561 tgacaagagt aacaaaaccg ggagcacaga acgtggcgat gaagcacggt cgaagcaaaa
    10621 ctccctcgaa gtactctcca gcggtaccgg tcgttttttc tctgatatcg cagactcata
    10681 taaaccaatc atcgttagtg tgaaaggcat atgcgcatcc gcatgctatt acgcggtatc
    10741 gcccgctgat gcaatttatg ccgacagtaa tgccctgatc ggttctatcg gtgtgcgtat
    10801 ggaccactgg aatctgtcag agatcatgag cacagtaggc gtcaagaatg agccccttac
    10861 ggccggtgag ttcaaagatg ctcttgaccc cttccaccca ttgtccgatt caacgaggga
    10921 gttcatgcag aaggagatcc tgaacacgat gcatgaaaaa tttataactg atgttgaact
    10981 tggacggggt aaaaagcttc tctcgcgtca tgatgccgat gctgtttctc tctattcagg
    11041 ccgtgtatgg ccaacacctc aggcagttaa gtacggcctg gtggatggag atcttacgtc
    11101 agtagagatc cgtacacgtc tttcaaaaat gtactccact gacacgttca aaaactacaa
    11161 tgagcctcat cgtaatctcc gttctgcact cggcatgctg atgagcctgt cttcaaatat
    11221 tgagagcctt accggtacca ctacccgctt ggtagaatct gttaatgcca ccagctaccc
    11281 ctctgtgagg taacatggat gctttcaatc tgctgtggag cattacgggg gtagctttca
    11341 ttatccttat tttcgtcgtg ttgctgtgtc tgttgggttt tatgacctct gccatagcgg
    11401 aacggcggac tgcaaaggca attgaatctg ggcttcctga agaagctcag gggctactta
    11461 gtgatctcac ctttcagttg tcggctcata gcactactca ggttgatcac attctcgtag
    11521 ccccacatgg tatatacgtc atagagcaga aaaactacgt aggcaagctc tacggtactc
    11581 tggaggaaag ccactggcgg aaatggactc agtcccgaac cctcaagttg cagaacccgt
    11641 ttaagcaaaa ccaggggcac atcagggcta ttcagtctgc tcttaaggct agagaactgg
    11701 aatgtatcaa tgtcgtcatc ataaacggac gttgtaagtt tgatggcatc aagccggaat
    11761 ggttatgtat ggggatggac gattttatcc ataaagtcaa acaacgacga gggctacggt
    11821 tgttcacacc ggagtctgtt cagcacatct gttcggtgtt gaagtcaaca aggaagtcgc
    11881 cagggctcta tacggacctt actcatattc acaacataac gacaaagtac aaagccccga
    11941 tgaaattcga gcaacgagta acatacattc tgctcaactt tatccattat ctgtgggcaa
    12001 gtctgttcac taagcagaag ccttagcgct ctatcattgg taaggctgta aaatgtctgc
    12061 aaaaggcccc atcggtaaat tgttggggct ttttctttgc gtagttctga tacggtttat
    12121 cctgtggagc attatggcca ccagccagct ggtgactggc caacgctttg tatgacgtag
    12181 cctactcagg gcaggctatt ttagagggac cgatttttat ccgggctaat ttagaaagag
    12241 ataatttgaa cttaggcggg ctgaccacca gcagagaggt aaatcactag ccggtgatat
    12301 tgacagaagt cgtcaggggt ttaacgccgg aatattttgc caggcctcct gcaaggtctg
    12361 caagacttct acaagtgata gtgacggcca caggactgtt tccgtcatta tcactccagg
    12421 tggctcgcac tctactggta ccagaattaa aacaacacga gttcctttta cctggtaggc
    12481 aaggtttact gccggttggc ggtaggaggc cagtacaccc tcaccatatt gagcagggtt
    12541 acttgcccag ttccgcacca ttgcatacac ctgaggcata taccgactat ccagatcccc
    12601 tttgggccgt ctgaacggca cgatttcttc tgagcggtag gtggtcgttg ttgtattggt
    12661 tgtcatcttt gtcacctccc ctgttcgtcc atttagagtt ttaagctgct ttacacatca
    12721 cactcaactg attatttaag ctggtcatcg tctctggcca gcatagccca accattcgac
    12781 tggttttcgc gggagtaagg attcatttca aaataactat ctaccgcgtc gctgtccgca
    12841 acagaactgt tgttagctct ctttgaaccc tgaaccacta cagcatcaac ctctgcggct
    12901 ttcccgttgc gctgtaccag gattttaacc gtcttgggct tacgtcttgc gagcagtttg
    12961 ttggcgataa aaagcacaac aatccaggcc aaaagcacgt ataaaaattt attcataatg
    13021 ccctcctttg tatgtgatta ttatacctgc ttccattgca ggccaaacag gttcggtgca
    13081 tgaaaaatag acttctctcc ggcgtatagc aatctactta ctgatctgtt ttcgtatcaa
    13141 actgtatggc ataaccgagg ggcgtacgtg gtgagtcaga aagttacttt tccgtaatag
    13201 ttaatttcag atccttcttc cagttacgct tcagtataaa accgataccc tctaattcag
    13261 catccttcac gtttaatacg cttcacttta aggcagggct tttcggttga tatgaattct
    13321 ttttccggtt gctggacatg ggtttcatcc tgatagttaa aattgcaggc t
//
LOCUS       DAPGOF010000130        13317 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.131, whole genome shotgun sequence.
ACCESSION   DAPGOF010000130 DAPGOF010000000
VERSION     DAPGOF010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.131"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(109..321)
                     /gene="cspH"
                     /locus_tag="RHV13_003399"
     CDS             complement(109..321)
                     /gene="cspH"
                     /locus_tag="RHV13_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HDV1242902.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISALTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            607..819
                     /gene="cspG"
                     /locus_tag="RHV13_003400"
     CDS             607..819
                     /gene="cspG"
                     /locus_tag="RHV13_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HDV1242903.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            830..1018
                     /locus_tag="RHV13_003401"
     CDS             830..1018
                     /locus_tag="RHV13_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDV1242904.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            993..1223
                     /gene="ymcE"
                     /locus_tag="RHV13_003402"
     CDS             993..1223
                     /gene="ymcE"
                     /locus_tag="RHV13_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HDV1242905.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLSRPDEVNSEY"
     gene            1213..1386
                     /locus_tag="RHV13_003403"
     CDS             1213..1386
                     /locus_tag="RHV13_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HDV1242906.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(1434..2507)
                     /locus_tag="RHV13_003404"
     CDS             complement(1434..2507)
                     /locus_tag="RHV13_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDV1242907.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQFLLLAMNI
                     YIAITFWYWVRYYETAGSTTFLARPGGIEGWLPIAGLMNLKYSLATGQVPSIHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQFVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVISLLSPFKIRRNAESCIDCGKCAKNCPSWIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFTVIGYAMYAG
                     VWQSPVPDELYRRLIPQAPMIGH"
     gene            complement(2579..5323)
                     /gene="torS"
                     /locus_tag="RHV13_003405"
     CDS             complement(2579..5323)
                     /gene="torS"
                     /locus_tag="RHV13_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HDV1242908.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGQRLQLRQQQQRLSQQIVAAADEIARLAQGQAN
                     NAATSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLKKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLTTEMADDVPTALMGDPRRIRQVI
                     TNLLSNALRFTDEGQIVLRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAIGNAAQALETLQNSEPFAAALVDFDLPDID
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQANNDLPLDVSQLNEDAQLMGTEKIHEWLALFKQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRSASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLNKKDLNAI
                     "
     gene            5406..6434
                     /gene="torT"
                     /locus_tag="RHV13_003406"
     CDS             5406..6434
                     /gene="torT"
                     /locus_tag="RHV13_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HDV1242909.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(6407..7099)
                     /gene="torR"
                     /locus_tag="RHV13_003407"
     CDS             complement(6407..7099)
                     /gene="torR"
                     /locus_tag="RHV13_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HDV1242910.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQVQPLTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            7229..8401
                     /gene="torC"
                     /locus_tag="RHV13_003408"
     CDS             7229..8401
                     /gene="torC"
                     /locus_tag="RHV13_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HDV1242911.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            8401..10947
                     /gene="torA"
                     /locus_tag="RHV13_003409"
     CDS             8401..10947
                     /gene="torA"
                     /locus_tag="RHV13_003409"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HDV1242912.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFARVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYVQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLVGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQTYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKL"
     gene            10944..11543
                     /gene="torD"
                     /locus_tag="RHV13_003410"
     CDS             10944..11543
                     /gene="torD"
                     /locus_tag="RHV13_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HDV1242913.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNELENCVATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LRQWLPEFVARCRQYDSFGFYAALSQLLLVLVEGDHQNR"
     gene            complement(11695..12000)
                     /gene="cbpM"
                     /locus_tag="RHV13_003411"
     CDS             complement(11695..12000)
                     /gene="cbpM"
                     /locus_tag="RHV13_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HDV1242914.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(12000..12920)
                     /gene="cbpA"
                     /locus_tag="RHV13_003412"
     CDS             complement(12000..12920)
                     /gene="cbpA"
                     /locus_tag="RHV13_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HDV1242915.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT         3248 a         3370 c         3484 g         3215 t
ORIGIN      
        1 ttaaatggat tagttttccg gtgaaaaagt aacttgtgtt ataatacatg gtgttgacta
       61 ttgaactatg ccaataaaac cagtcagctt taaacaagca gcgtcatatt aagagagata
      121 aacatttgcc gctgttggtc ctcgcaggcc atttacgcgg caaaattcca cgcgtaatcc
      181 tggtataagc acttctgcgt cgcggggagt taatgcggaa atatggacct gaacttcttt
      241 acgaccgtcg gaggggataa tgaatccttt gccgcttttg cgatcaaagg ttttgacaat
      301 tcctgtcatt ttacgggaca aacaaattcc ttactgaaaa tactgcgctg cactatacgg
      361 ggttaataaa ataaagccag cgatatttaa gaccgccgga cggctaaaat aaaatttgct
      421 taatctcaat tatcatgcgt taatagctgc gtcggtttga aagacagaca gcatacaaag
      481 tagtttacta aagcagttct cattatcagg cattatcccc ttcttttgag tctctctcct
      541 gaacactaag tagtttctgt attaaagccc tgtttgccga aaggcccaaa atgaaggaag
      601 taaaatatgt ctaataaaat gactggttta gtaaaatggt ttaacgcaga taaaggtttt
      661 ggctttatca ctcctgatga tggcagcaaa gacgttttcg tccatttcac cgccatccag
      721 agcaatgaat tccgcacgct gaacgaaaat cagaaagttg aattttctat tgagcagggg
      781 caacgtggcc ccgcggcagc gaacgttgtt acgctctaag gttgccgtta ttactcaaca
      841 tctccatttc cgctgtccat gttgtcatgg ttcacagtac cgcacatcag cattcgatgt
      901 gacggagcga aaccctttgg gcgctaagtg tattttttgt aaatcgacga tgatcacctt
      961 tgataacgtc gcgctgcaaa tacgcactga ccatgcgccg ctggatttca caaaataata
     1021 tcaggctccc tcgtggagcc ttttttatat ctgccttatt tttcttcaac gctgtatgta
     1081 tagtaagcga taacctgttg attattgaat ctttcgggga gatggcttat aacatttctt
     1141 acctgaccag ggtaccggga accaacacct tactggcgtg ttgctgtctt tcaagaccag
     1201 acgaggttaa cagtgaatat tgaagagtta aaaaaacaag ccgaaacgga aatcgccgac
     1261 tttatcgcgc aaaaaatcgc cgagctgaac aagaatacag ggaaagaagt ctctgaaatt
     1321 cgcttcaccg cacgagaaaa aatgaccggg cttgaaagtt atgatgtcaa aatcaaaata
     1381 atgtgatttt gtgaacatca cccgtgcgag gtgatgttcc gcttgttgct aatttagtgg
     1441 ccaatcattg gcgcttgtgg aattaagcgt cggtacaatt catccggtac cgggctttgc
     1501 catactcccg catacattgc gtaaccaatc accgtaaaca taatccccag aaccagtagc
     1561 gtcattaacc agccagacaa cgcaaaggct tttttatttg ccgcaggttt ttgcagtgaa
     1621 aaggtcaatg ttgaggctac cggacatgac tctacgcaag tcatacagcc ggtacattcc
     1681 actgttcgta cctgaattaa tttatcgacc gggatccatg atgggcaatt ttttgcgcat
     1741 ttgccacagt cgatacaact ttcggcattg cgacgaatct taaacggcga taatagcgaa
     1801 atcacgccca tcagcgcgcc atatgggcaa agataacgac accaggcatg gcgaataaac
     1861 aggctggcaa tcagcaaaac ggtcacgctg attaatgtcg cggtccccat atgacgaaag
     1921 aaatcgagca ttttaacgtc catcaccacg ctgtagggcg acaacataaa atagtgaatc
     1981 gcctgagcgg gcatcaataa cgcgatatag agaaaaaaac tcaacagcaa atacttcacg
     2041 ccgcgcagag gaatatccag ccagcgggga aggacaaatt gccgaccaaa cagtttgtta
     2101 ccgagatcgc cgattaattc agaaagcgta ccaaccgggc ataaccatga acaaaaggcc
     2161 tttttgagta atagactgat gacgataaaa gcgaccaata acagcatcgc ggcggcgtgg
     2221 atggacggta cctgacctgt tgcaaggcta tatttcagat tcatcaaacc ggcaatcggt
     2281 agccagcctt cgatacctcc cggtctggcg agaaatgtcg tactacctgc cgtttcgtaa
     2341 tagcgcaccc aataccagaa cgtgatggca atataaatat tcattgccaa tagtaagaat
     2401 tgcgtcgctt tacgccaggt cgtggcattg cgccagtcat tccacggtaa tttgccgccc
     2461 gtcgtgcctg gccgccgctg ccagcgggtt ctttttttct ctgccatgat tttgccagtc
     2521 cgttaagttg cataccaaat gccactattc tagttattct taactggctg atattgattc
     2581 aaatcgcgtt caggtctttc ttattcaacc atgcttccag agcggcaaca ctgcgtgtaa
     2641 tttcttcgtg tggaaggggg gcagataatg gctgctgctc cagttgtgcg catagctgac
     2701 tggcgctacg cattcccaga ctcgagcaac tgctttttag ctgatgcgcg gcacgcttta
     2761 ttttctcgct gtcctggctg gcgcgggcaa tgtcgatttc atcgagaagc ggcagggcat
     2821 gttgtttaaa taatgccagc cattcgtgga tcttctccgt ccccattaac tgagcgtctt
     2881 cattgagttg cgagacatcc agtggtagat cgttattggc ttgcagttgg agatagtgcg
     2941 ccagtaactg accgagcact tcacgcggca ccggtttagg gataatcccg cgaaatagtg
     3001 aactggtacg ctggcgcagc gtttcgtcaa tgacatgggc gctaaagcca atcaaaacca
     3061 gcgacggata ttgctgtgcc agttgccggg caagggtaat gccgtcgata tccggcagat
     3121 caaaatccac cagtgcggca gcaaacggtt cgctattttg cagtgtctct aaagcctgcg
     3181 cggcattgcc aatagcaaca acctgcgcac cactggtgtt cagcatctca acggtaattc
     3241 gctgggttag cgggttatct tcaattaaca gcaaacgtaa accgtcaaga cgcaccgcct
     3301 gattgactgt ttttggcacg ggtgccgtgg caacacgtaa cggcaagcgt aaacaaaaac
     3361 agcttccaac ctccggcgtg ctggtggcac tcagttcgcc gcccatcgcc tgggccagac
     3421 ggctactgat agtcagcccc agcccggtgc cgccgcgttt gccgcttacc tgcacaaatg
     3481 gctggaagat ttctgccagt ttcgcgggat caataccgca gccgctgtct tccacttcga
     3541 ccagccattg ctcgccatca gtgcggctac gtaaaacgat ctgcccttcg tcagtaaaac
     3601 gtagggcgtt gctcaacagg ttggttataa cctgacgaat acgtcgcgga tcgcccatta
     3661 acgcagtcgg tacatcgtcg gccatttcag ttgtcaggcg aattggccga cctttcaccc
     3721 gtccgctcat taattgcagg gtactttcca gcagcgggcg cggttcaaag ggttcatcgc
     3781 tgaccgaaac attcttgcca cctgcttcga tagccgaata atcgagaata tcgttgagga
     3841 tggtcagcaa cgattcgcca gagtcggtaa tcgcccgcaa atcatcacgc tgggcgttaa
     3901 gtgcggggtt atctgccagc agttgagcag tgccgagaat accgtacagc ggtgtgcgga
     3961 tctcatggct catcgccgcc agaaacgccg attttgcctg gctggctttt tctgcttccg
     4021 cccgtgcctg tcggtgttct atcaccagtt cctgcaattc agctgtacgc gctttgacct
     4081 gcgccgccag ctgttcgcgg tggcgattca gtgcatgaac actgctgcga aacgcatcca
     4141 tcagccgccc gatggtatcc agctcccgta cgccagcggt ttccgggaaa ggggagtcaa
     4201 tatcaccgtc cagcagccgt tgcagcgcct gcgtttgttc ggcaagtgga cgcgtgactg
     4261 agcgataaac cacgcgccag aggatcagaa tcagggcgca aagtgaaacc atccccagca
     4321 ataacaggct gtattgcccg cgtgcactgg ctttttccag atgcgccagt ccatgctgat
     4381 tgcgcagctc aatggtgtcg accagctgac tgacttcgct actaaactgc gcgaactggg
     4441 cgatattatt ttgagcgaga gtttgcaggt gattgctgat ttcactgtcc tgctggaaca
     4501 gcgccagcaa atcgctatat tggctaacgg tagttaacgt tgttgcgacc tgagcacgaa
     4561 ctcccggatc ttcaatgcgt atttgccgac gttgcagaat tttcaccgca ttattgagct
     4621 gctttttcag cgttggtgca tttttctgga tctgctccag ccccagattc atcaccattt
     4681 gctgcacccg cagagcgcta aggcgcagtt cattcatctg gttaacatac tcaagatcga
     4741 tatcaatcag ccgatcgagt gcactttcag cagcctgacg ctgatcttgt tcgatcaaat
     4801 cgtaaatccc ggcctgggtc gctccggcgg aggttgccgc attattcgcc tgaccttgcg
     4861 ccaggcgtgc gatctcatcg gcggcggcga ctatctgctg actgagtcgc tgttgttgct
     4921 ggcgcagttg caaacgctgc cccaccagtt ccccttgctg acgtaacgaa cgggagatct
     4981 cctgctcctg ttgttcaata gcggtggtat caaaaccttg ttcccgtaac gcttgcagca
     5041 acgcattaat cttcaggctt tgtgcggtga gcattcgccc ctgcgcctgc cacatctttt
     5101 cgttatcggc actggtcagg ttctgcgcgg cgaaaagttc ccaggcgctg gcttcgctca
     5161 actggcgcgc catattcatg gtaggaatca atgcctgagt attgtctttt tccacctggc
     5221 tgataaagcg caggttgtac catcccacca gggtactggt cagggttaac agcgccatca
     5281 gggcaaagcc catccagagt cttcgggtca gggttaaatt cacggtcggt gtacttaagg
     5341 tgaaaaaggt tgagtcgcaa agcggaatgc atctagcata aagccttatt attgatgagg
     5401 ctatcatgcg cgtactgcta tttttacttc tttccctttt catgttgccg gcattttcgg
     5461 ctgataacct gttgcgctgg catgatgcgc agcatttcac ggtgcaagcc tctatgccgc
     5521 ttaaagccaa acgcgcatgg aaactgtgcg cgctttatcc gagcctgaaa gattcatact
     5581 ggttatcgtt gaactatggt atgcaggagg ctgctcgccg ctacggtgtg gatttaaaag
     5641 tgctggaggc aggcggctac agccagttgg ctacccagca agcacaaatc gaccagtgta
     5701 aacagtgggg cgcagaggcc attttgctcg gtagtagcac gacgtcattt cccgatctgc
     5761 aaaagcaggt agcaagtctg ccggtgatcg aactggtaaa tgctattgat gctccccagg
     5821 tgaaaagccg cgttggtgta ccctggtttc agatgggcta tcaacctggg cgatatctgg
     5881 tgcaatgggc gcacggtaaa ccactgaacg tgctgttgat gcccggaccc gataacgccg
     5941 ggggcagtaa ggagatggtc gagggttttc gcgcagccat tgccggaagc ccggtgcgta
     6001 ttgttgatat tgcgcttggt gataacgata ttgaaatcca gcgtaacctg ttgcaggaga
     6061 tgctggaacg ccatccagaa atcgacgtcg ttgccggaac ggccattgcg gcagaggcgg
     6121 caatggggga agggcgtaac ctgaaaacgc cgcttaccgt ggtgtcgttt tatctttcac
     6181 atcaggtgta tcgcgggctg aagcggggaa gagtgattat ggctgccagc gatcaaatgg
     6241 tctggcaggg ggaactggcg gttgagcagg ccatcaggca attacaggga caatcggtat
     6301 ctgataatgt cagcccaccg attttagttc tgacgccgaa aaatgccgac cgcgaacata
     6361 tccgccgctc gctgtcgcca gggggatttc gtccggtcta tttttatcag cacacatcag
     6421 cggctaagaa ataaccttcg ccatgttgcg tcaccagtaa atccgcgctg agtttatggc
     6481 gtaaacgacg aattaacaca tcgacggtgc gcaggtcagg gttttccacc cgacgcgcag
     6541 agagcatacg cagcagacgt tcacggctga gaatttctcc cggattcgtc acaaatgcca
     6601 ccaacatttc atactctgcg cgggtcagtt taatcggctc accatcccgc tccagcgtat
     6661 ggcgcgacac attcaggcaa taacccgcaa agcgatagca gttgtcctga gtgagcggtt
     6721 gaacttgtcg cgcgaggtcg attcgccaga gcagattttt tacccgtact accagttcgc
     6781 gcagttccag cggtttggtg acgtaatcgt ctgcgcccat ttccagccca acaatacggt
     6841 caatccgatc gctgcgtccg gtaaccagaa taatccccac cgttgagcgt tctcgcaggg
     6901 cgcgggttaa catcaggcca ttttcatcgg gcaagttgat atccagcaga attaaatcca
     6961 ccggctgatt ctgcataatt tcccgcagcc cggcaccgct cgccgtaacg gaaacggtat
     7021 acccctcctg agtgaagtag gattgtaatc gcgcctgggt aaccggctca tcttcaacaa
     7081 taacaatgtg atgtggcatc agagggtttt actcattctg ttcatatctg ttcatattct
     7141 gccgtaagcc attcatcctg accagtgccg ctgttcatat ttgctcatta agatcgcttc
     7201 actaaaccat aattttacag gggctattat gcggaaactc tggaacgcgc tacgccgacc
     7261 cagtgctcgt tggtcggtac tggcgctggt cgctattggg attgtgattg gcattgcgct
     7321 gattgtattg ccacacgttg ggatcaaagt caccagcaca accgaatttt gtgtcagttg
     7381 ccacagtatg caaccggtgt atgaagaata taaacagtcg gtgcatttcc agaacgcctc
     7441 cggcgtgcga gctgaatgcc atgactgtca tatcccgccg gatattccag gcatggtgaa
     7501 gcgtaaactg gaagcgagca acgatatcta ccagaccttt attgcccact ccattgatac
     7561 acctgagaaa ttcgaagcca aacgcgcgga acttgccgag cgtgaatggg cgcgaatgaa
     7621 agaaaacaac tcggcaacct gccgctcctg ccataactac gacgcgatgg atcatgcgaa
     7681 gcagcatcct gaagcggcac gtcagatgaa ggtggcagcg aaagataatc aatcctgcat
     7741 cgactgtcat aaaggtattg cccaccagtt accggatatg agtagcggct tccgtaagca
     7801 gttcgatgag ctgcgcgcca gtgctaatga cagtggtgac acgctgtact ctattgatat
     7861 taagccgatt tatgcggcga aaggcgataa agaagcctca ggttctctgc tgcctgcctc
     7921 ggaagtgaaa gtccttaaac gtgacggcga ctggctgcaa attgaaatta ccggctggac
     7981 agaaagcgcc ggacgtcagc gtgtactcac ccaattccca ggtaagcgca tctttgttgc
     8041 ctcgattcgt ggtgatgtgc agcagcaggt gaaaacgctg gagaaaacca ccgtagccga
     8101 caccaatacc gagtggagca agttgcaggc caccgcgtgg atgaagaaag gcgacatggt
     8161 gaacgatatc aaaccgatct gggcttatgc tgattcgttg tacaacggca cctgtaacca
     8221 gtgccacggc gcaccggaaa tctcccactt tgacgctaac ggttggatcg gcacgctcaa
     8281 cggcatgatt ggctttacca gcctcgataa acgtgaagaa cgcaccttgt tgaaatatct
     8341 gcaaatgaat gcgtctgaca ccgcaggtaa ggctcacggc gataagaagg aagaaaaata
     8401 atgaacaata acgatctctt tcaggcatca cgtcggcgtt ttctggcaca actcggcggc
     8461 ttaactgtcg ccgggatgct ggggccgtca ttgttaacgc cgcgccgtgc gactgcggcg
     8521 caagcggcga ctgaggctgt catctcgaaa gagggcattc ttaccgggtc gcactggggg
     8581 gctatccgcg cgacggtgaa ggatggtcgc tttgtggcgg caaaaccgtt cgaactggat
     8641 aaatatccgt cgaaaatgat cgccggattg ccggaccacg tacacaacgc ggcgcgtatt
     8701 cgttatccga tggtacgcgt ggactggctg cgtaagcgcc atctgagcga cacctcccag
     8761 cgcggcgata accgttttgc gcgcgtgagc tgggatgaag ccctcgacat gttctatgaa
     8821 gaactggagc gcgtacagaa aactcacggg ccgagtgcct tgctgaccgc cagcggttgg
     8881 caatcgacgg ggatgttcca taacgcttcg gggatgctgg cgaaagctat tgccttgcat
     8941 ggtaatagcg ttggtacggg tggggattac tctaccggtg ctgcgcaggt gatcctgccg
     9001 cgcgtggtcg gttcgatgga agtgtatgaa cagcaaacct cctggccgct ggtattgcag
     9061 aacagcaaaa ccattgtgct gtggggctcc gatttgctga aaaaccagca agcgaactgg
     9121 tggtgcccgg atcacgatgt ttatgaatat tacgtgcagt tgaaagcgaa agtcgccgcc
     9181 ggtgaaattg aggtcattag catcgatccg gttgttacat ccacccatga gtatctgggg
     9241 cgcgagcatg tgaagcacat tgcggttaac ccgcaaactg acgtgccgct gcaactggcg
     9301 ctggcgcata cgctgtacag tgaaaacctg tacgataaaa acttcctcgc taactactgt
     9361 gtgggttttg agcagttcct gccgtatctg gtgggcgaga aagacggtca gccgaaagat
     9421 gctgcatggg ctgaaaaact gaccggcatt gatgccgaaa ccattcgtgg gctggcgcgg
     9481 cagatggcgg cgaacagaac gcagattatt gctggctggt gcgtacagcg tatgcagcac
     9541 ggtgaacagt gggcgtggat gattgtcgtt ctggcggcga tgttggggca aattggcctg
     9601 ccaggtggtg gctttggttt tggctggcac tataacggcg caggcacgcc ggggcgtaaa
     9661 ggcgttattc tgagtggttt ctccggctct acgtcgattc cgcctgttca cgacaacagt
     9721 gattacaaag gctacagcag caccattccg attgcccgtt ttatcgatgc gatcctcgaa
     9781 ccggggaaag tgatcaactg gaacggtaaa tcggtaaaac tgccgccgct gaaaatgtgt
     9841 atttttgccg gaactaaccc attccatcgc catcagcaga tcaaccgcat tattgaaggc
     9901 tggcgcaagc tggaaacggt tatcgccata gataaccagt ggacctcaac ctgccgcttt
     9961 gccgatatcg tgctgcctgc gaccacgcag tttgagcgta acgatctcga ccagtacggc
    10021 aaccactcca accgtggcat tatcgccatg aaacaggtcg tgccgccgca gttcgaggcg
    10081 cgcaacgact ttgatatttt ccgcgagctg tgccgccgct ttaatcgcga agaagccttt
    10141 accgaagggc tggacgaaat gggctggctg aaacgcatct ggcaggaagg tgttcagcaa
    10201 ggcaaaggac gcggcgttca tttgccagcg tttgatgact tctggaataa caaagagtac
    10261 gtcgagtttg accatccgca gatgtttgtt cgccaccagg cattccgcga agatccggat
    10321 ctcgaaccgt tgggcacgcc gagtggcctg attgagatct actcgaaaac catcgccgat
    10381 atgaactacg acgattgtca ggggcatccg atgtggtttg agaaaatcga acgctcccac
    10441 ggtgggcctg gctcgcagac gtatccgttg catctgcaat ctgtgcatcc ggatttccga
    10501 cttcactcgc agttatgtga gtcggaaact ctgcgtcagc aatatacggt agcgggtaaa
    10561 gagccagtgt tcattaaccc gcaggatgcc agcgcgcgcg gtattcgtaa cggtgatgtg
    10621 gtacgcgtct ttaacgctcg cggtcaggtg ttggcagggg cagtcgtttc tgatcgctat
    10681 gcacccggcg tggcgcgaat tcacgaaggg gcatggtacg atccagataa aggcggcgag
    10741 cctggtgcgc tgtgcaaata cggtaacccc aacgtgttga ccatcgacat cggtacttcg
    10801 cagctggcgc aggcgaccag tgcgcacact acgctggtgg aaattgagaa gtacaacgga
    10861 acagtggagc aggtaacggc gtttaacggc cccgtggaga tggtggcgca gtgcgaatat
    10921 gttcccgcgt cgcaggtgaa attatgacca cgctgacagc acaacagatt gcctgtgttt
    10981 acgcctggct ggcgcagttg ttctcccgtg aactggacga tgaacaactg acgcaaatcg
    11041 ccagtgcgca gatggctgaa tggttttcgt tgctgaaaag cgaaccgccg ctcactgcgg
    11101 cggtgaatga gctggaaaac tgtgttgcca ctctgacagt acgtgacgat gcccgtctgg
    11161 aactggccgc tgacttttgc ggcctgtttc tgatgactga caaacaagcg gcgctgccat
    11221 atgcatcggc ctacaaacag gacgagcaag agattaaacg cttgttagtt gaggcaggga
    11281 tggaaaccag cggcaatttc aacgaaccgg cagatcatct ggcgatctat ctcgagttgt
    11341 tgagtcatct gcatttttcg ctgggagagg ggacggttcc tgcgcgaaga atcgacagtt
    11401 tacggcaaaa aacactcacg gcgctgcggc aatggttacc agagtttgtt gcgcgttgtc
    11461 gtcagtatga cagctttggt ttttacgcgg cactaagcca gttattgctg gtgttagttg
    11521 agggcgacca ccaaaacaga taacgtcgtt tgttggcctg ataagacgcg tttagcgtcg
    11581 catcaggcat tagggcaccg ccgccggatg cggcgtgaac gccttatccg gcccacagga
    11641 aaagtactct ttgcaggcag gctaagatgc gtcatcacat ccggcaaaca cacatcacgg
    11701 atgagctaca aaccgggaaa gccgctggcg cagcaggcgg ttttcctgct tcaggtgcgc
    11761 aatatcatcc attaacgtca gcgccaccgc gatccccggc cagtccagag ccagttcatg
    11821 acgcaggcgc accgcgcgtt gcaccacaat ggcggcatgg tcgtcaaata cccaggttgt
    11881 ttcctggatc tcacgcggtt caaccacccc caaaccgaca atttcattca attcctcttc
    11941 agagatgccg gtatgcaggc aaaattcggt aatagtaaaa gtcaccgtaa cattagccat
    12001 tatgctttcc cccaatcttt acgtggatca aaagacgact gggcgtctgc cagttgctgc
    12061 cacagcgcgg cagtgttttc atccggtttc ggcggcatca cgattttcag taccgcatac
    12121 agatcgccgg tctgtttttt gctcaccaga cctttgcctt taacgcgcaa tcgttgcccg
    12181 gcctggctgc caggcgggat agtcagcaaa atgctttctt tcagtgttgg aacggtgact
    12241 ttagcaccca gcgccgcttc ccacgggcta accggcacca caatttccag atcctggccg
    12301 acaatatcaa acagcggatg tggcgcaata tgaatcacca gccacaaatc gccatttgga
    12361 ccgccgtttt cgcccggcgt cccctggcct ttcaggcgga tgcgttgacc attgccgacg
    12421 cctgccggga tcttcacatt cagcgttttc gggatttcct gttcgatcat gccaaaggcg
    12481 ttataaaccg gcaggttata gctgatggta cgcttatgct cagtaagcgt ttcttcgagg
    12541 aataccgcca cttcgatttc aatatcgtgg ccgcgtgtgg cggggcgttg acggctctgg
    12601 cgggcatgct gaccgaaaat tgacgagaag atatcgtcaa aatcttcggc attaaaactc
    12661 tgaccgtcgc catggtggaa ctgacggtta aattgcggat cgttgcgatg ttgccacatc
    12721 tgatcatact cagcgcgacg ttgttcatca cttaacactt cccaggcttc agcgacctct
    12781 ttgaagcggg cttcggcatc cggttctttg ctgacatcag gatggtattt acgggccagt
    12841 cgacgatagg cggtcttgat tgtcttgaga tcgtccgtcg gtttcacgcc catgatggcg
    12901 taataatcct ttaattccat agcgttatct cgcgtaaatc aacacaaatt gaaggaaccc
    12961 ctgtaaggta actcctataa gtgtagggta atcctcaaaa tttcatatgc caacacagaa
    13021 tatgttattg aaatcatcgc gtagaggagg tcgcctaaag gtggggtgct gaacatattt
    13081 taaacaggtg aaaaagggtg agcgattttt gatagttgaa ccaggcactt taagtttaaa
    13141 tagggcgtca ttgtttatta aattttatag gcgctatata tgggtagtaa tatacatgga
    13201 attagttgca ctgcaaataa ttatttgaaa caggcctgga acgatataaa aaatgagtac
    13261 gaaaaaaatc aaacatattc aatcacgctt tttgaaaaca cactggtgtg ttttatg
//
LOCUS       DAPGOF010000131        13204 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.132, whole genome shotgun sequence.
ACCESSION   DAPGOF010000131 DAPGOF010000000
VERSION     DAPGOF010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.132"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            169..1668
                     /gene="pitB"
                     /locus_tag="RHV13_003413"
     CDS             169..1668
                     /gene="pitB"
                     /locus_tag="RHV13_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HDV1242916.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLITMEPPLPAASTDSAQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(1717..2409)
                     /locus_tag="RHV13_003414"
     CDS             complement(1717..2409)
                     /locus_tag="RHV13_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HDV1242917.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLDVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            2583..3296
                     /locus_tag="RHV13_003415"
     CDS             2583..3296
                     /locus_tag="RHV13_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HDV1242918.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     ERIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCRSQQGHLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEILQASLRAIHAALS"
     gene            3328..4086
                     /locus_tag="RHV13_003416"
     CDS             3328..4086
                     /locus_tag="RHV13_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242919.1"
                     /translation="MDALQTQTVISTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIASDRRSLT"
     gene            4132..5481
                     /locus_tag="RHV13_003417"
     CDS             4132..5481
                     /locus_tag="RHV13_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HDV1242920.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFVGQCLDVVLSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            5481..6305
                     /locus_tag="RHV13_003418"
     CDS             5481..6305
                     /locus_tag="RHV13_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242921.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVNRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSGFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPVALDSDIRQRLELLISRFPL"
     gene            6317..6877
                     /locus_tag="RHV13_003419"
     CDS             6317..6877
                     /locus_tag="RHV13_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HDV1242922.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLDKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            6908..7978
                     /gene="lptF"
                     /locus_tag="RHV13_003420"
     CDS             6908..7978
                     /gene="lptF"
                     /locus_tag="RHV13_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HDV1242923.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFTLLQKFSR"
     gene            7975..9054
                     /gene="lptG"
                     /locus_tag="RHV13_003421"
     CDS             7975..9054
                     /gene="lptG"
                     /locus_tag="RHV13_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HDV1242924.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSMGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWILHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMDLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPIAFVLVALSLVYWYDRQH"
     gene            complement(9089..10261)
                     /locus_tag="RHV13_003422"
     CDS             complement(9089..10261)
                     /locus_tag="RHV13_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDV1242925.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAIAEGQTALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(10261..10509)
                     /locus_tag="RHV13_003423"
     CDS             complement(10261..10509)
                     /locus_tag="RHV13_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDV1242926.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(10541..11455)
                     /locus_tag="RHV13_003424"
     CDS             complement(10541..11455)
                     /locus_tag="RHV13_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDV1242927.1"
                     /translation="MNQTVAVTGATGFIGKYIINNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYANSKYVAEQRLTAMADKITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVQQLAADARCGSVRMVGIPLPVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(11452..13185)
                     /locus_tag="RHV13_003425"
     CDS             complement(11452..13185)
                     /locus_tag="RHV13_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HDV1242928.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSSAGMNFYDRRC
                     QLEDQLEYQTLKARAEAGAKRLLSLNLKKGDRVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNNPELHVLSHAW
                     FKALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRP
                     GDRCVSWLPFYHDMGLVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSV
                     APPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTF
                     MPCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKAL
                     PEHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQASQDEIAATGWLDTGDLGYL
                     LDGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQC
                     RISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAY
                     AASLHVQESLA"
BASE COUNT         3159 a         3277 c         3428 g         3340 t
ORIGIN      
        1 atgcctgtgt tctaattatt tctaaagatt tttaatgaat gcttattgtc tgatacacaa
       61 gaaataacac tctttttatc gttaaaaaat gatatttcac tttgcccaca ccgttaaagt
      121 cactgataat gcgtccgttc gtaaattcaa aatggcgtaa tctaatatat gctaaattta
      181 tttgttggcc ttgatatata cacagggctt ttgttattac ttgctctggc atttgtgttg
      241 ttctacgaag caatcaatgg ttttcatgac acggcgaatg cggtggcaac cgttatttat
      301 actcgtgcca tgcaaccaca acttgctgtg gtgatggcgg cattttttaa cttttttggc
      361 gtgttattgg gcggacttag cgttgcctat gccattgtcc atatgttacc aaccgatttg
      421 ttgctgaata tggggtcaac ccacggcctg gcgatggtct tttccatgct gctggcggcg
      481 attatctgga acctgggaac gtggttcttc ggtttaccgg cctccagttc gcacaccttg
      541 attggtgcga ttatcggcat cggtttaacc aacgcgctgt taaccggctc atcggtgatg
      601 gatgcgttaa acctgcgtga agtgaccaaa attttctcct cgctgattgt ttcccctatc
      661 gtcggcctgg tcattgcggg aggcctgata ttcctgctgc gacgctactg gagcggaacg
      721 aaaaagcgtg accgtattca ccgcattccg gaagatcgca aaaagaaaaa aggcaaacgt
      781 aagccgccat tctggacgcg tattgcgctg attgtttccg ctgcgggcgt ggcgttttca
      841 cacggcgcta acgacgggca gaaagggatc ggcctggtga tgctggtact ggtggggatt
      901 gcccctgctg gcttcgtcgt caatatgaac gcgtccggct atgaaattac ccgtacccgc
      961 gatgccgtca ccaacttcga acactacctg caacagcatc ctgaactgcc gcagaagttg
     1021 attacgatgg aacctccatt gcctgcggca tctactgata gcgcgcaagt gacggagttt
     1081 cactgtcatc cggcaaatac ctttgatgcg attgcgcgcg ttaaaacgat gctgccaggc
     1141 aatatggaaa gttacgagcc gttaagcgta agtcagcgca gccagctgcg ccgcattatg
     1201 ctgtgcatct ctgatacctc cgcgaagctg gcgaaactgc caggcgtcag taaagaagac
     1261 cagaacctgc tgaaaaaact gcgcagcgat atgttaagca ccattgagta cgctccggtg
     1321 tggatcatca tggcggtagc actggcgctc ggcattggca ccatgattgg ctggcgtcgt
     1381 gttgcgatga ccatcggtga gaagattggt aagcgcggca tgacgtatgc gcaaggcatg
     1441 gcggcacaaa tgacggcggc agtgtctatc ggtcttgcca gttatattgg gatgcccgtc
     1501 tctacaacac acgtactctc atctgcagtt gcagggacga tggtggtgga cggcggtggg
     1561 ttacagcgta aaacggtaac cagcatcctg atggcgtggg tatttacttt accggcggcg
     1621 atttttcttt ctggtgggct gtactggata gcattgcagt tgatttaacc ttcctgaaaa
     1681 tgcccggtcc tggcgatcgg gcatttccat ttttggctag tgataaccac gcgcggtcat
     1741 aaaatccgta atcgcttttt ctgcatcaac caacacctgt tccagtggct gattggcatc
     1801 gatatcaacc agttgtgcac caccaaaggt taactgtggc gttatggcaa tcttcctcgc
     1861 cagcgattcc cgtttatggt cgggtttacg cgcgcaggcc acgtctagat cgacattgag
     1921 tttgatgacc agatcaggct tatggctcgc catccagtga aacgctttac gttcctggct
     1981 tgccagccat gagacaaaac gaccaccttc aacgttaggt gggaacaccg taccatcgta
     2041 agcgccagga atttggtcct gaggataacg gtcggttaga acaattaacc cgcgacgacg
     2101 acaggcaagc atatgacgaa agcgcagtaa gcgacgggcg acaaacgctg taattaccag
     2161 cgccggaact ggtcctggca atttttttgc tgttttcacc tgatttcgtt caattgtttt
     2221 atgtaaggat tttcccatca acggtaattt tgtcactgca cgaccgacat ttccggcttg
     2281 ttttcctaaa tgaactcttt cggcagcacc atatttttcg acaacggtaa tgagatgttc
     2341 acacaccgtt gacttgccag aaccatcgct accaataacg gcaattaatg gaggtgttat
     2401 tgactgcatg aatatatcct taaatttaaa tatccatcca aaatatttat ttggttaata
     2461 tgtttttatg aaagcgtaat tcaggtcaat gtcacaatta accattgtca caataaggtt
     2521 gaacggatat tctgagcacg gaccacgtaa tattcaatac attatttact gccgggtttt
     2581 tcgtgaatca acgtaatatg tcaataatta attccacgcc agtgcgtgtt attgccattg
     2641 taggatgtga tggttcaggt aaatcgaccc tcacggcaag cctggtaaat gaactggcag
     2701 caagaatgcc aacagaacgc atttatctcg ggcaatcgtc cgggcgaatt ggcgaatgga
     2761 tttcacagct ccctgttatt ggcgcacctt ttgggcgtta tctgcgaagt aaagcggcac
     2821 atgtgcacga aaagccctca acaccgcctg gcaatattac tgcgctggtt atctatctgc
     2881 tttcctgctg gcgggcgtac aagtttcgca aaatgttgtg tagaagccag caaggccatc
     2941 tgctcatcac cgaccgctac ccgcaggttg aagtgccggg ttttcgcttt gatggcccgc
     3001 aattggcaaa aaccacgggc ggtaacggtt ggataaaaat gttaaggcag cgcgagttga
     3061 agctgtacca atggatggcg tcttatttac ccgtattgtt gattcgtctt ggcattgatg
     3121 aacaaaccgc gtttgcgcgt aaacctgacc accaactggc agcgttacag gaaaaaatcg
     3181 ccgttacgcc gcaattgaca ttcaatggcg caaagatcct tgaactggat ggtcggcaac
     3241 cagccgatga aattctgcaa gcgtcactac gcgcaattca cgccgcgctt tcctgatcac
     3301 caaaatggat aggggatgat ttaacgaatg gatgcactac aaacacaaac tgttattagt
     3361 acaaccgcgc cgcagcccaa ctacattccg gggctgattg cggtggtcgg gtgtgatggc
     3421 accggtaaat ccacactgac caccgaccta gtgaaatcgc tgcaacaaca ctggcaaacc
     3481 gagcggcgct atctggggct gctctccggc gaagacggcg acaaaatcaa acgattgccg
     3541 ttggttggcg tctggctgga acggcgactg gcggccaaat cctcgaaaac ccaaagcatg
     3601 aaaaccaaat ctccggcgct atgggcggcg gtgattatgt actgcttctc gctgcgaaga
     3661 atggcgaatc tacgcaaggt tcagcgactg gcgcaaagtg gcgttctggt ggtcagcgat
     3721 cgcttcccgc aggctgaaat ttcgggcttt tattatgacg gaccggggat tggcgtcgaa
     3781 cgtgcgaccg ggaaaatcag catgtttctg gcgcagcgcg aacggcgttt ataccaacaa
     3841 atggcgcaat atcgcccgga attaattatt cgcctgggca ttgatattga gactgccatc
     3901 tcccgcaagc ctgaccatga ctatgccgag ctgcaggaca aaatcggcgt catgtcgacg
     3961 attggctata acggcacaaa aattcttgag attgattcca gagcgcccta cagcgaagtg
     4021 ctggaacagg cacagaaggc ggtttctctg gttgccatcg cttctgaccg ccgaagttta
     4081 acttaaggct ggcgggatac tttttactat caacgataag gtcaggctga attggcgggt
     4141 tttaacatca aacattggtt tgcagatggc gcgtttcgca ccattattcg caatagcgcc
     4201 tggttaggct ccagtaatgt cgtgagcgcc ttgctggggc tgttggcgct ctcgtgtgcc
     4261 ggtaaaggga tgacgcccgc catgtttggc gtactggtga ttgtgcaatc gtacgccaag
     4321 tcgatcagcg attttattaa gtttcagaca tggcagctgg tggttcagta cggaacgcca
     4381 gccttgacca acaataatcc gcagcaattt cgcaatgtcg tctcattttc cttctcgctg
     4441 gatatcgtca gcggcgcggt ggcgattgtc ggcggcattg ccttactgcc attcctttcc
     4501 cattcattag gtctggatga ccaaagcttt tggctggcgg cgctgtattg cacgctcatt
     4561 ccttcaatgg cttcctccac gccgaccggc attctgcgtg cggtagatcg ctttgatttg
     4621 attgctgtac agcaggcgac gaaacctttt ctgcgcgcag cggggagcgt cgcagcctgg
     4681 tattttgact ttggttttgc gggttttgtt attgcctggt acgtgtcgaa tctggttggc
     4741 ggcaccatgt actggtggtt tgccgcgcgc gaattacgcc gcagaaatat ccataacgcc
     4801 ttcaaattga atctgtttga gtctgcccga cacattaaag gcgcgtggag ttttgtctgg
     4861 tcaaccaaca ttgcccactc catctggtcg gcgcgtaact cgtgcagcac agtgttggtg
     4921 gggatcgtgt taggacccgc tgccgccggg ttatttaaaa tcgccatgac attcttcgac
     4981 gccgccggaa cgccagcggg tttgctgggt aaaagttttt acccagaggt gatgcgttta
     5041 gatccgcgca ccaccagacc gtggttgtta ggcgtgaagt ccggtttact ggcgggcgga
     5101 atcggtattc tggtggcgct cgcggtgttg atcgtcggca agccgctcat ttcgctggta
     5161 tttggcgtta agtatctcga agcgtatgac ctgattcagg tgatgctggg cgcaattgtg
     5221 atctcgatgc tgggcttccc gcaggagtca ttgctattga tggcgggaaa acagcgcgct
     5281 tttctcgtgg cgcaaaccat cgcctcaatc ggctacatcg tattgctgtt catgttctgt
     5341 catctgtttg gcgtgctggg ggctgcattt gcttacttcg ttgggcaatg tctggatgtg
     5401 gtgctctcgt taattccaac cttaaaggca ttttttcagc gccattcctt gctttataac
     5461 gcagccggag agaaatcctg atgatgagaa aatatttccc gcttgaggcg agcgagcgtc
     5521 tatttgttgc gattgaagaa gatgatgtcg ttgatgcaca ggttagtttg ccgccgacta
     5581 ttgcactaag ttgtaccacc gaaatcattc atgataatta cgcgttatgc ctgcaattct
     5641 ggctcaatgg cgttaatcgt caggaattac tgcgtttagt tcgcaaacaa gccaaaggcg
     5701 atgagctgac tgctgatgag cgcaaacaat ttaaatacat gcgagcgcgc tacaagcacc
     5761 tgcgttttgc gcaacggctg tatttaaaga agcatcaggc cggattttta ttcggcaaaa
     5821 cgaccgtttt tctgggacgt tttcaggacg gatttcgtaa cggtaagaaa aatattgtgt
     5881 cgtattacgg caacctgtta cgaatttatt taagctctcc ggtctggtcg ttggtgaact
     5941 attcattacg ccatagccag ttagaaagcg taagcggttt tattgcctat cggcaaaagc
     6001 aaatgcatac cctgaaagag attattgcca aaccgcgact aaccggcagg gaatttcatg
     6061 atgtgcgaaa aatcatcagc cagcaggttt cttattacga caccttaaga tcgcttgatc
     6121 ctgaaaataa agaggcgtta cagatttccc gtttcctcgc cgccattaat ggcctgatgg
     6181 gggataaaca cgacgacatg gtagcggatg atatggaaaa ccgccagtcg tatgatgcac
     6241 cggtggcgct ggatagcgat attcgccagc gcttagagct gctgatttcg cgttttccgc
     6301 tataaccgga gcaatgatgt catctcgttt gattattgcg ctgattatca tgctcttagc
     6361 cccgggtgtg caggcacaca atttcgtcac tggcaaaacg gttacccctg tttatattca
     6421 ggaaggcggc gagctgcttc tgaatagcga cgacgaaatt cactaccaaa agtggaacag
     6481 cacacaattg gcgggaaaag tccgcattat ccagtatatc gccgggcgta agtcggcgaa
     6541 gaaaaagaac tcactgttaa ttaaagcggt tgaagcggca aactttccgc aggatcgttt
     6601 tcagcccacc accattgtga acaccgatga tgccatcttt ggtactggct attttgttgt
     6661 tggcaaaatc gaaaaaaata aacgccgcta tccgtgggcg caattcgtta tcgacggcaa
     6721 tggacaggga cgcgttgcct ggcgcttgcc ggagcagagt tcaaccattc tggtgctgga
     6781 taaagccggg caaattcagt gggcaaagga cggcagcctg acgccggaag aggtagatca
     6841 tgtcattgcg ctggcgcaga aactgatcaa tgaataaaaa attcaaagga ttgagggcgt
     6901 tagtgagatg aagctggttg aacactacat catgcgtggt acgcgccgtc tggtgctgat
     6961 tatcgtcggt tttctgatct ttattttcgc cagctactcc gcacagcgtt atctgaccga
     7021 agcggcaaat gggacattag ctcttgatgt tgtgctggat atcgttttct acaaagtgct
     7081 gattgcactg gagatgttgt tacctgttgg tctgtatgtg tcagttggcg tgacgctagg
     7141 gcagatgtat accgactcgg aaattaccgc tatctctgcg gcggggggca gtccgggacg
     7201 cttgtacaaa gccgttcttt atctggcgat accgctaagt atttttgtca cccttctgtc
     7261 gatgtatggt cgtccgtggg cttatgcgca gatttatcaa ctggagcagc agtcgcagtc
     7321 ggagctggat gttcgccagt tgcgggcaaa gaaatttaac actaacgata acggacgaat
     7381 gatcctttcg cagacggttg atcaggataa taatcgcctg actgacgcgc tgatttatac
     7441 ttctactgcc aatcgaaccc gcattttccg cgcccgttcg gttgatgtgg ttgacccatc
     7501 acccgagaaa ccgaccgtta tgttgcacaa cggcactgcc tatcttctcg atcatcaggg
     7561 tcgtgacgac aacgaacaga tctaccgtaa tctgcaatta catctgaatc cgctggatca
     7621 aagccctaac gtcaaacgca aagcaaaatc ggtcacggag ctggcgcgct ccgcctttcc
     7681 tgccgatcat gccgaactgc aatggcgaca aagccgtggc ctgacagcat tgttgatggc
     7741 gctgctggcc atttcattaa gtcgggtaaa accgcggcag gggcgatttt caacgttatt
     7801 gccactgacg ttgctgttta ttgccatttt ttatggcggc gacgtctgcc gtacgctggt
     7861 ggctaacggc gcgattcctc tcattcctgg tttgtggtta gtgcccggac tcatgctaat
     7921 gggcctgctg atgctggttg cacgcgactt cactttgctg cagaaatttt cccgatgaat
     7981 gttttcagtc gctatttaat ccgtcatctc tttctcggtt ttgccgccgc cgcagggcta
     8041 ttgctgccgc tttttaccac cttcaacctg attaacgaac tggatgatgt cagcccgggc
     8101 ggttatcgct ggactcaggc ggtgctggtg gtgctaatga ccttaccgcg cacactggtc
     8161 gaactttcgc cgtttatcgc cttattggga gggattgtcg gcctggggca gttatcgaaa
     8221 aacagtgagc ttaccgccat tcgcagcatg gggttttcta tcttccgtat tgcactggtg
     8281 gcgctggttg ccgggatatt gtggactgtt tcgttaggcg cgatagatga gtgggtagcg
     8341 tcgccgttgc agcaacaggc gctgcaaatc aaatcgaccg ccactgcgtt gggggaggac
     8401 gatgatatta ccggcaatat gctttgggcc aggcgcggta atgaatttgt aacggtgaaa
     8461 tcgctgaacg agcagggcca gccggtgggc gtggagatat ttcattatcg cgacgatctc
     8521 tcgctcgaat cctacattta tgcacgcagt gccaccattg aagacgacaa aacgtggatc
     8581 ctgcatggtg tgaatcataa aaaatggctg aatggcaaag aaacgctgga aacattagat
     8641 aatcttgcct ggcaatcggc cttcaccagt atggatcttg aagagttatc aatgccgggc
     8701 aatacttttt ctgtccgtca gcttaatcat tacatccatt atttgcagga aaccgggcaa
     8761 cccagcagcg aataccgcct tgcactgtgg gaaaaactgg ggcaacccat cctgaccctg
     8821 gcgatgattt tgctggctgt gccgtttacc tttagtgccc cgcgctcgcc agggatgggt
     8881 agccgtctcg ctgtaggtgt catcgttggc ttactcacct ggatcagcta tcaaatcatg
     8941 gtcaatctgg gattgctatt tgcgttgagc gcacctgtta ccgcgctcgg tttaccgata
     9001 gcgtttgtgc tggtggcgtt gagcctggtg tattggtatg acagacaaca ttaaacccgc
     9061 cgtggcgcaa caccacagcg ggctcacatt actgcttcag agtagcgaac gcttgaatga
     9121 tggcatcaat ttgctcacga ctgtgggcag cgtttacgct acagcgcagc agcgtgatgc
     9181 ctgcaggcgc ggcaggtggc aggataagat tcacataaac gcccagcgaa ataagctcgc
     9241 gccagatgcg taaaccttcc tctttcgaac cgattattac cggcactacc gggctaatat
     9301 gtgaacttaa ctcataaccc agtttttgca gaccgtcata cagatggttg gcgttatcca
     9361 tcagcttttg ccgcagctct ggttgtgtgg caatggttct taacgacgag cgcacggtcg
     9421 caatgcagga cggagaagga gaggcggtaa aaatatacgg gcggctgccg tagcgcagca
     9481 cttccatcgc ttccgaaccg acagcaaaac cgccaatcga ggccagactt ttactgaagg
     9541 taccaacgat aatatccaca tcatcttcaa cgccaacggc ttcagccagt ccgcgccccg
     9601 ttgcgcctaa cacgccaaac gaatgcgctt catcgacaat caaataacca ccgagacgac
     9661 gtttgatatc aacaatttcc gccagcggcg caacgtcacc gagcatgctg tagatgccct
     9721 caacgatgat aatcgcctct ttcgcccgtt caccgaggcg aaccatgcgc cgttccagat
     9781 ctttagcatc gttatggcgg aaacgaataa tttccgcgcc accgagagaa caggcgtcat
     9841 aaatactggc gtggctgtcg gcatccagta acaccacggc gttatggtcg gcaagtgcgc
     9901 tgatgacgcc taaattagcg gtataaccgg ttgaaaaaac gatagcggta gggcgattga
     9961 aaaactcggc aatctctttc tctaacgcca ggtgcggcgc ataactgccg tttgccatac
    10021 gagaaccggt ggtgccggtg ccttgggccg ctaacgctgt ctgaccttcg gcgatggcat
    10081 catgattaaa ggttaaaccg aggtaattat tggtcccggc cagaatcact tttttattgc
    10141 cgatccggcc ttcggtggcg gaatacactt catcaataca ggtgccaaac ggtgttaacc
    10201 cattgtcact aaattgcagt cgctcgccag caaggcgcgc atatttatca tatagcccca
    10261 ttatttattc tccagccatg gaagtagagt ttccattaat tgcgcagggg ttttgacatc
    10321 cagcagaata ttaataggaa tggaaatatc gaatctgtct tccagcatca ttaagagatc
    10381 catcactttg atcgactcca ggccaagatc gttgacgaga tcgctgtcgg gcttaatttc
    10441 cacaccgttt tcgaccaaat cctgtaaaca ggaaaggatg taatccatta ctatttcacg
    10501 atttaccata gaaacgttaa cacaccttta aacaagacaa tcaaaatagc ccgttgcgta
    10561 atgcgtgttc caggctaatc ccgggaaacc aattaatatc ttcagaaata cggttattac
    10621 ttgccgacca gtcggcgtgg gttaattcac gaattttcga gcgggtcagc ataggttctt
    10681 tacctgccag gcggctcaac gcggtactga tatccgcaag gcaggtgagt accggcagag
    10741 gaatgccaac cattcgcacg gaaccacaac gggcgtcggc ggcaagttgc tgtacgcgtt
    10801 gccagtcata gccgccagcg acgccatcgc ataattcata ggtttgcgtc tgtacagttt
    10861 cggcgcttaa ccactgaccc actgcttgcg cgaaatcggt gacgtgcagg aaagagagct
    10921 gtgtatctgg cgcaccaagt cgtggcagca ggccgcgcag catccagtca aacagcggtt
    10981 ttaactcttt atcgccgggg ccatacacgg ctgtcgggcg aaaaacgccc agcgtaattt
    11041 tgtcagccat tgcagtcagc cgttgttcgg cgacgtattt ggaatttgcg taccaggaga
    11101 gttcgggatg gcgcgccgcc agcgaagaga taaacagaaa acgttggcaa aaaccgctct
    11161 cttttgccgc ctgcatcagg cgcagactgc cgtcaacgtt acagcgggtg aaaatctctt
    11221 ctttgtgccc gcgcacttgt ccggcgcaat gaaccaccgc gctggctccg gcaaccagtt
    11281 cgctaagcga atgtgtatct tccagcgaac cgcgcaccca ggtgagatta tcattgacgt
    11341 gagcgcgggc agtacgcgtc aatgcgcgaa cgtgaaagcc gcgggcgagc aggttattga
    11401 taatatattt accgataaac ccggtagcgc ccgtcaccgc gacagtttga ttcatgccag
    11461 ggattcctgc acatgaaggc ttgcagcata agccttctga taacgttttt tcgcttccgc
    11521 acgggcaggc ttgccggagg acgttcgggg aatgctgtgg ggcggcaaca gatcgatagc
    11581 cgcggtcacg ccaaattcgc tttggatccg tgctgccagc gcgtggataa gctgcccgcg
    11641 acgttcttca tcgctgatcc gacactggat ctgcaaaatg attttttcct gggcggtaac
    11701 aaaagcaatc gcatcgccag aatgaatttc cggttcctgt tccgctatat attcaatatc
    11761 ctgcggccag atattacggc cacgaataat aatcagatct ttaatgcgtc cggtgacata
    11821 cagataaccg tccagcagat aaccgaggtc gccagtgtct aaccagcccg tcgcggcaat
    11881 ctcgtcttgc gaagcctggt cgccaaagta accgctcatc agactgggac cggagatgca
    11941 aatatggcct accacacgtt ccgcgaccgg catacctgct tcattgcgga tttcaatacc
    12001 atgttccggc aacgctttgc cgcagttgac gaaagtcgat acggcgcgtg tctctgcacc
    12061 cggcgcgacg gctttaccct gatattcgag gatgtcgcga tccacttcgt taaccacaac
    12121 cccggaggct tcatcagaga agctgacagc cagcgcattt tctgccagtc cgtagcacgg
    12181 catgaaagtt ttattgtcaa agttaacctg acggaaacat tcagcgaatt gatggagttg
    12241 ttctgcggag atgggttctg caccaatacc agcgacgcgc cagcaggaaa gatccagttc
    12301 agcgagatct ttttcattca cgcggcgctg gcacaattca tagccaaacg gcggcgcaac
    12361 ggaaacggtg ccgcgatttt tactgatcaa tttaagccat tgcagaggac gcatggcaaa
    12421 atcctgagtg cgcaaataat ctactgaaag ttgcgtggcg acgggggtca ggagaaagcc
    12481 gaccagtccc atatcatggt agaaaggcag ccaggagacg cagcggtcgc cagggcgtaa
    12541 tttaatgcca tcgtggctta tggcgcgtag attagccatc acttcgcggt gagtgataat
    12601 gacgccacgg ggaaaacggg tgctgccgga ggtgtactgg aggtaggcga tatcgttcgg
    12661 aactggacgc tggagcgcaa catcggcttc cggtaatgcc ttaaaccagg cgtggcttaa
    12721 aacatgtaat tcggggttgt tgttatgcgt cgcggcattg accagcggta accactcatc
    12781 accagtgata atggctgcgg gctggcaact tgccagtaaa ccctgcagtt tggcgctcca
    12841 ggaatcccgc tgaccgacgc ccattggaat cgccaatggt acggcaacta atccagcata
    12901 ctgacaggca aaaaatgcct cgacaaaccc gctactggtt tcggcaatca gcgccacgcg
    12961 atcgcctttt ttcaggttta gcgataacaa ccgcttcgca ccagcttcgg cacgcgcttt
    13021 taacgtctga tattccagtt gatcttcaag ttggcaacgt ctgtcataaa aattcattcc
    13081 agcgctactc agagcggcgt agtccaaagc atcaaccagc gttggaaaat cggcatagcg
    13141 cataggtagg gaatgcgtaa agattttatt agacatatac accacgaaaa aattggatat
    13201 ccaa
//
LOCUS       DAPGOF010000132        13071 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.133, whole genome shotgun sequence.
ACCESSION   DAPGOF010000132 DAPGOF010000000
VERSION     DAPGOF010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.133"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            379..2010
                     /gene="oppA"
                     /locus_tag="RHV13_003426"
     CDS             379..2010
                     /gene="oppA"
                     /locus_tag="RHV13_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HDV1242929.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2096..3016
                     /gene="oppB"
                     /locus_tag="RHV13_003427"
     CDS             2096..3016
                     /gene="oppB"
                     /locus_tag="RHV13_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HDV1242930.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3031..3939
                     /gene="oppC"
                     /locus_tag="RHV13_003428"
     CDS             3031..3939
                     /gene="oppC"
                     /locus_tag="RHV13_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HDV1242931.1"
                     /translation="MMLSKKSSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3951..4964
                     /gene="oppD"
                     /locus_tag="RHV13_003429"
     CDS             3951..4964
                     /gene="oppD"
                     /locus_tag="RHV13_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HDV1242932.1"
                     /translation="MSVIEPATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4961..5965
                     /gene="oppF"
                     /locus_tag="RHV13_003430"
     CDS             4961..5965
                     /gene="oppF"
                     /locus_tag="RHV13_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HDV1242933.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6014..6343)
                     /locus_tag="RHV13_003431"
     CDS             complement(6014..6343)
                     /locus_tag="RHV13_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HDV1242934.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6378..7838)
                     /gene="cls"
                     /locus_tag="RHV13_003432"
     CDS             complement(6378..7838)
                     /gene="cls"
                     /locus_tag="RHV13_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDV1242935.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDTRLWLKRPLWQRVAERLFYFFSPLL"
     gene            7981..8154
                     /locus_tag="RHV13_003433"
     CDS             7981..8154
                     /locus_tag="RHV13_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HDV1242936.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8209..9462)
                     /gene="kch"
                     /locus_tag="RHV13_003434"
     CDS             complement(8209..9462)
                     /gene="kch"
                     /locus_tag="RHV13_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HDV1242937.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(9762..10058)
                     /locus_tag="RHV13_003435"
     CDS             complement(9762..10058)
                     /locus_tag="RHV13_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HDV1242938.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            10282..11001
                     /gene="tonB"
                     /locus_tag="RHV13_003436"
     CDS             10282..11001
                     /gene="tonB"
                     /locus_tag="RHV13_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HDV1242939.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            complement(11041..11439)
                     /gene="yciA"
                     /locus_tag="RHV13_003437"
     CDS             complement(11041..11439)
                     /gene="yciA"
                     /locus_tag="RHV13_003437"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HDV1242940.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(11544..12083)
                     /locus_tag="RHV13_003438"
     CDS             complement(11544..12083)
                     /locus_tag="RHV13_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HDV1242941.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(12113..12856)
                     /locus_tag="RHV13_003439"
     CDS             complement(12113..12856)
                     /locus_tag="RHV13_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HDV1242942.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAVLLIYLFRLYMLIRQ"
     gene            complement(12884..12973)
                     /locus_tag="RHV13_003440"
                     /pseudo
     CDS             complement(12884..12973)
                     /locus_tag="RHV13_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
BASE COUNT         3502 a         3112 c         3227 g         3230 t
ORIGIN      
        1 ttaagacatg gatgcaagct atagattctg atacggtcaa taaaagagaa ttgcttaaca
       61 attttgcaaa atgtattggc gagtaagaag cgcatttggt actttccggg caaccgccag
      121 gcgattcttt attggtaatg agaataatta acaattaaag agcgtcgtga aagaataatg
      181 tgtctcgaca ggggagacac agtacgaatc gacataaggt gatcgtctga atcaccagaa
      241 taaataaagt cggtgatagc aatacgtaac gataaagtaa cctgacagca gaaagtctcc
      301 gagcctgtgc agggtcccaa tccgggatta cacatgctgg ttaataccag taattataat
      361 gagggagtcc aaaaaacaat gaccaacatc accaagagaa gtttagtagc agctggcgtt
      421 ctggctgcgc taatggcagg gaatgtcgcg ctggcagctg atgtgcccgc aggcgtcaca
      481 ctggcggaaa aacaaacgct ggtacgtaac aatggttcag aagttcagtc attagatccg
      541 cacaaaattg aaggtgttcc ggagtctaat atcagccgag acctgtttga aggcttactg
      601 gtcagcgatc ttgacggtca tccagcacct ggcgtcgctg aatcctggga taataaagac
      661 gcgaaagtct ggaccttcca tttgcgtaaa gatgcgaaat ggtctgatgg cacgccagtc
      721 acagcacaag actttgtgta tagctggcaa cgttctgttg atccgaacac tgcttctccg
      781 tatgccagtt atctgcaata tgggcatatc gccggtattg atgaaattct tgaagggaaa
      841 aaaccgatta ccgatctcgg cgtgaaagct attgatgatc acacattaga agtcacctta
      901 agtgaacccg ttccgtactt ctataaatta cttgttcacc catcaacttc accggtgcca
      961 aaagccgcta tcgagaaatt cggcgaaaaa tggacccagc ctggtaatat cgtcaccaac
     1021 ggtgcctata ccttaaaaga ttgggtcgta aacgaacgaa tcgttcttga acgcagcccg
     1081 acctactgga acaacgcgaa aaccgttatt aaccaggtaa cctatttgcc tattgcttct
     1141 gaagttaccg atgtcaaccg ctaccgtagt ggtgaaatcg acatgacgta taacaacatg
     1201 ccgatcgaat tgttccagaa gctgaaaaaa gagatcccgg acgaagttca cgttgatcca
     1261 tacctgtgca cttactatta cgaaattaac aaccagaaac cgccattcaa cgatgtgcgt
     1321 gtgcgtaccg cactgaaact aggtatggac cgcgatatca ttgttaataa agtgaaggcg
     1381 cagggcgaca tgcccgccta tggttacact ccaccgtata ctgatggcgc aaaattgact
     1441 cagccagaat ggtttggctg gagccaggaa aaacgtaacg aagaagcgaa aaaactgctg
     1501 gctgaagcgg gttataccgc agacaaaccg ttgaccatca acctgctgta taacacctcc
     1561 gatctgcata aaaagctggc gattgctgcc tcttcattgt ggaagaaaaa cattggtgta
     1621 aacgtcaaac tggttaacca ggagtggaaa acgttcctcg acacccgtca ccagggtact
     1681 tttgatgtgg cccgtgcagg ctggtgtgct gactacaacg aaccaacttc cttcctgaac
     1741 accatgctgt cgaacagctc gatgaatacc gcgcattata agagcccggc ctttgacagc
     1801 attatggcgg aaacgctgaa agtgactgac gaggcgcagc gcacagctct gtacactaaa
     1861 gcagaacaac agctggataa ggattcggcc attgttcctg tttattacta cgtgaatgcg
     1921 cgtctggtga aaccgtgggt tggtggctat accggcaaag atccgctgga taatacctac
     1981 acccgaaata tgtacattgt gaagcactaa tggcaatacg tggggcagga gtgtcctgct
     2041 ccacggtgtc tgatttttat cgcattacag aaggcacagg ccagaaggta gggcaatgtt
     2101 aaaatttatt ttacgtcgct gtctggaagc gattccgacg ctatttattc ttattactat
     2161 ttcgttcttt atgatgcgcc tcgcgccggg aagtcctttt accggcgaac gtactttacc
     2221 gccagaagtg atggccaata tcgaagcgaa atatcatctt aatgatccga tcatgacaca
     2281 gtatttcagc tacctgaaac aactggcgca cggtgatttc ggtccatcgt ttaaatataa
     2341 agattattcg gtcaatgacc tggtggcatc cagttttccc gtttctgcca aactgggagc
     2401 cgcagcattt ttccttgcgg taatactggg tgttagtgct ggcgttattg ccgcattaaa
     2461 acaaaacacc aaatgggact ataccgtgat ggggctggca atgaccgggg ttgttatccc
     2521 cagttttgtg gttgcgccat tattagtcat gatatttgcg atcattttgc attggctgcc
     2581 gggcggtggc tggaatggtg gggcgcttaa attcatgata ttgccgatgg tggcgttgtc
     2641 actcgcctat atcgccagta ttgcgcgtat tacccgtggc tctatgattg aagtattaca
     2701 ctccaacttt attcgtactg cccgggcgaa agggttacct atgcggcgga tcattttacg
     2761 ccacgcatta aaacctgctc tgttacccgt gctctcctat atgggccccg catttgtcgg
     2821 cattattacc ggttctatgg ttatcgaaac catttatggt ttgccgggga ttgggcaact
     2881 gttcgttaat ggcgcattga accgtgacta ttccttagtg ttaagcctga ccatcctggt
     2941 tggtgcgtta accattttgt ttaatgccat tgtcgatgtg ctatatgcgg ttatcgaccc
     3001 gaaaatccgt tactgagact ggagctcgcg atgatgttaa gtaagaaaag cagcgagacg
     3061 ctggaaaatt tcagtgaaaa gctggaggtc gaagggcgca gcttgtggca ggacgcacgt
     3121 cgacgtttta tgcataaccg tgcggcggtt gcaagtctga tagtgctggt gctgatcgcg
     3181 ttatttgtaa tcctggcacc gatgctttcg cagtttgcct atgacgatac tgactgggcg
     3241 atgatgtcca gcgccccgga tatggagtcc ggtcactact ttggtactga ctcatccggt
     3301 cgcgacctgc ttgtgcgcgt tgcgattggc gggcgtatct cactcatggt cggtgttgct
     3361 gcggcactgg tggcagtggt cgtggggaca ctttacggtt cgctttccgg ttatctgggc
     3421 ggtaaagtgg attcggtaat gatgcgtctg ctggaaatcc tcaactcctt cccattcatg
     3481 ttcttcgtca ttttgctggt gacctttttc ggccaaaaca tcctgctgat tttcgtggcg
     3541 attggcatgg tttcctggct ggatatggct cgtattgtgc gtgggcaaac cctgagtctg
     3601 aagcgcaaag agtttattga ggcggcacaa gttggcggtg tatcgacgcc gggcattgtt
     3661 attcgccaca ttgtgccgaa cgtactcggt gtggtggtgg tctacgcatc gctactggtg
     3721 cccagcatga tcctctttga atctttcctt agcttcctgg ggttgggtac gcaagagccg
     3781 ttaagcagct ggggggcatt gctgagtgat ggcgcgaact cgatggaagt ctctccatgg
     3841 ttactgttgt tcccagcggg attcctcgtg gtgacgctgt tttgtttcaa ctttatcggc
     3901 gatggcttgc gtgatgccct cgacccgaaa gatcgttaag gagtgcagcc atgagcgtaa
     3961 ttgaacctgc aactgtgccg ctcgcacaac aacaggctga cgcactgctg aacgtgaaag
     4021 atttgcgtgt cacctttagt accccggacg gcgacgtcac ggcggtcaat gatttaaatt
     4081 tttccctacg tgccggagaa acgctgggca ttgtaggtga gtctggttcg ggtaaatcgc
     4141 aaactgcatt tgcgttgatg ggcctgttgg ctgccaacgg acgcattggc ggatcggcaa
     4201 ccttcaatgg gcgtgaaatc ctcaatttgc cagagcgtga actcaataaa ctgcgcgctg
     4261 aacaaatctc aatgattttt caggacccaa tgacttcgtt gaatccctat atgcgcgtcg
     4321 gtgagcagtt gatggaagtg ctgatgctgc ataagaacat gagcaaagct gaggcgtttg
     4381 aagagtcggt gcggatgctc gatgcggtaa aaatgccgga agcgcgtaaa cgcatgaaaa
     4441 tgtacccgca cgaattttct ggcggcatgc gtcagcgagt catgattgcg atggcattgc
     4501 tatgtcggcc taagctgctg attgcggatg aacccactac tgcgctggac gtcaccgtac
     4561 aggcgcagat catgacgcta ttaaatgaac tgaagcggga atttaatacc gccatcatta
     4621 tgatcaccca cgatcttggc gtggtggcgg ggatctgcga caaagtgctg gtaatgtacg
     4681 ccgggcgcac gatggaatat ggcaacgcgc gcgatgtctt ttatcaacct gttcatcctt
     4741 attctatcgg tttgctcaac gcggtgcctc gtctcgatgc ggaaggtgaa acaatgttga
     4801 ctattccggg taatccgcca aacctgctgc gattaccgaa aggttgcccg ttccagccac
     4861 gttgtccgca tgcgatggaa atttgtagta gcgcaccgcc gctggaagag tttacgcctg
     4921 gccgtctgcg tgcttgcttt aaaccggtgg aggaactgtt atgaatgctg taaccgaagg
     4981 aagaaaagtc ctcctcgaaa tcgccgatct gaaagtgcac tttgaaatca aagatggcaa
     5041 acagtggttc tggcaaccgc cgaaaacgct caaagccgtc gatggtgtca ctcttcgcct
     5101 gtatgaaggg gaaacattag gtgtggtagg ggaatcggga tgcggtaagt ccacctttgc
     5161 tcgcgccatc atcggtttgg tcaaggcgac cgacggtcat gttgcctggt taggtaaaga
     5221 gttgctgggc atgaagcccg atgaatggcg tgccgttcgc agtgatattc agatgatttt
     5281 ccaggatccg ttggcatcgc taaacccgcg tatgaccatc ggcgagatca tcgctgaacc
     5341 actgcgtact tatcatccga aaatgtcacg ccaggaagtt cgcgagcgcg tgaaggcgat
     5401 gatgctgaaa gtcgggttat tgcctaacct gattaaccgc tatccgcatg agttctctgg
     5461 tgggcagtgc cagcgtatcg ggattgctcg tgctcttatt cttgaaccga agctgattat
     5521 ctgtgatgag ccggtgtcgg cgctggacgt gtcaattcag gcacaggtgg tcaacctgct
     5581 ccagcaattg caacgtgaga tgggattgtc attaattttt atcgctcatg acctggccgt
     5641 ggtaaaacac atttccgatc gtgtgttggt gatgtatctc ggccatgcgg tagaactggg
     5701 gacctatgat gaggtctacc acaatccact acatccttac accagggcat tgatgtcggc
     5761 agtccccata cctgatccgg atctggagaa gaacaaaacc atccagttac tggaagggga
     5821 attaccgtcg ccgatcaacc cgccttccgg ttgtgttttc cgtacccgtt gcccgattgc
     5881 cggtccggag tgcgccaaaa cacgtcctgt tctggagggg agtttcagac acgccgtttc
     5941 ttgcctgaaa gtcgatccgc tttaaacaca ataagggctg acagttgtca gccctttttc
     6001 acgccgaaag cgattattcc cgccagatga tatggcaaag tttgtgatct ttttcgcggc
     6061 ataacagaat gcgggcgaaa acatcgttga tttcaccatc ttcactgtcc gccagaccaa
     6121 tcaccacttc ggcaaaaaag tctgggttca aatcgaaatc aacatgttcc tgccagtctt
     6181 ccgccggatc gaataactcc gcgccgccgc gctcttcaaa ctgaagattg aacagcagaa
     6241 catcagctgg atcgagattg tcggcagcca gttcgagaaa aatatcgtag gcctgctcaa
     6301 gcgtttcatc ttcagtcagg cgattgttta gatccatatc catagtcact acctgtttaa
     6361 cctctgttgg cgacgtttta cagcaacggg ctgaagaagt aaaacagtcg ctcggcgaca
     6421 cgttgccata atggacgttt tagccataaa cgggtatcga gcagacgtga acgcgaaata
     6481 taatcgtcct gaacggcggc aaggtctgca ccaaaacctt tatcgtcgat tgccagggta
     6541 atctcgaaat tcagccacag actacgcata tcaaggttaa cggtgccaac cagacttagt
     6601 tcgccatcga ccagcacgct cttggtatgc agtaacccgc cttcaaactg ataaatttta
     6661 accccagcag caagcagttc cgtaaagaat gcgcgactgg cccagccgac cagcatcgag
     6721 tcattttttc gcggaaggat aatactgaca tccaccccgc gctgcgccgc cgtgcaaatc
     6781 gcatgaagta aatcatcgct tggcacaaag tagggcgtgg tcatgatcaa atattcacgc
     6841 gccgaataag ccgcagtcaa taatgcctgg tgaatgagat cttccggaaa gccggggcca
     6901 gaagcaattg tgtgaatggt atgaccgctg gcctgctcaa acggcataat attgacatca
     6961 ggtggtggcg gcagaatacg tttccctgtt tcaatctccc agtcgcagga ataaataatc
     7021 cccatcgcgg tggcgatggg gccttccata cgcgccatca gatcaatcca ttgccctacg
     7081 cccgcatctt gtttgaagta gcgagggtcg accatattca tgctgccggt gtacgcgatg
     7141 taattatcga tcatgatcat cttgcgatgt tggcgcaggt ccatacggcg taaaaacaca
     7201 cgcatcagat taacctttaa ggcttcgacc acttcaatac cggcattacg cattagctcg
     7261 ggccacgggc tgcggaaaaa agctacactc ccggcggagt cgagcatcaa tcggcaatga
     7321 atgccgcgtc gcgcagccgc cattaatgat tcagccacct ggtccgccat gccgccgggc
     7381 tgccagatat aaaacaccat ctcaatatta tggcgcgcga gctggatgtc gcggattaac
     7441 gcctgcatca catcatctga ctcggtcatc agttgtagct gattcccttt gaccccagcg
     7501 atcccctgac gacgctcgca aagcttgaat aatggcgcag cgacactgct attttcttcg
     7561 gcgaagatat gcttacaggc tttaaggtcg ttaagccatt ttgcggtgga aggccacatc
     7621 gctctggcgc gctcagcgcg gcgtttgcct aaatggagct cgccaacggc aagataggca
     7681 ataattccga ctaacggcag aatgtaaata atcaacagcc aggccatcgc ggagggaact
     7741 gcgcgtcgtt tcattagaat gcgtaaagtt acgcctgcaa tgagcaacca gtatcccaga
     7801 atggccaacc aactcaccaa cgtataaacg gttgtcatag attagaaatc cttttgaaag
     7861 cgcattgtta tgagtttacg catatggatt catctggcaa ataaaaacgc gggaaaagca
     7921 ctggtcagcg tcgggggtgg gggtataatg accattctgt tattgcatag agtagttaac
     7981 atgaagcgga gtagaacgga agtggggcgc tggcgcatgc agcgtcaggc tagccgacgt
     8041 aaatcgcgtt ggcttgaggg gcaatcgcgc cgaaatatgc gtatccacag catcaggaag
     8101 tgcattctaa acaaacagcg taactcgtta ttgtttgcga tctacaatat ctaaatgtac
     8161 tgggcaccat tgcggtgccc aataagttta ctcttccgtc tttaaactct atttttgcgc
     8221 cgattcttta ctgtcagctt tcgtttcttg ttcatcgtta tcgctgaaaa taccatgacc
     8281 tgagttcaac aacattgaaa cgagcatatc attattaatc tcttcaccat ttaaaactcg
     8341 cgccagaatt tcgctgccaa acagttgcgg tgagagaatg atatccggat gtaccatctt
     8401 aatcttattc aggtttttgc tatcactgac ggcgagaact gttttgacat cactgctcat
     8461 atcttttgcc gagagtacaa caaacgcgtt atctgcatcg ttatcactca gcgccagaat
     8521 ggcccggcaa cgatcgattc ccgctttctt taatactgaa ctgtcattac tgtcaccggg
     8581 gataacatca gcgttatcgc ctaagcgttg ctcaagttgc ttgatatcat cttcaggcaa
     8641 gttgctgata accgttacgt tttgtccgcg ttgattcagt tgcaggatcg tattgatggc
     8701 gagaatcgaa tgtccgcaaa caataaaatg atctttacga tgcattgtat gattgtttcc
     8761 ttttacaagt ttgttgaatc ccccgcggat aagcggacca aaaattgagg tcatagatgt
     8821 ggcaaaaacg gtaatgccgg aaataatgac cgaaatagtg aacaatcgtg ctgattcaga
     8881 aacagggaca atatcgccat agccgacggt tgacatggtt tctatcgaaa aatagaacgc
     8941 ggtcatcaaa ctttctattc gcggattaaa accttcgctt aaataaagcg caccgtaggt
     9001 ggagtaaaac agtaacgtcg tgaaactaat aaatgcaaaa attgtcccgg ctgcggcgct
     9061 actgtgggag aagtctttgc gcagtatcag caaaaacacc agcgtaaaaa tgcaaaatcc
     9121 aatactaaat tttagccagg gataaaaatg cagggtgtaa attagcgcta tcaacaacaa
     9181 aatgatactg attgcccatg cgagctttgc ccggaacagt aagccaatgg agttcaggac
     9241 aagaaagacg ccgagcataa atagtggggc gtttgccagc aaggagagat ccaactctga
     9301 aaatgctttg atatggaaaa tatcaagcaa attgaccgac ataccataga ttgttttaaa
     9361 aataagcaat ccatttaaaa agacggccag cgcgagaatg tcgtgccgta gggtcaccca
     9421 taaatttgtt gctgtttgtt tgaatgtagc ccagtgactc actgatacct ctgtaaccac
     9481 aattttgttt aattatctta gcgtgcacat tttctgaaca tacatgcagc gcgtgaatgt
     9541 gttaaacaat agtagatgac tttatccgca gtaatcaaag gttttgagcg agcaactgta
     9601 cctcaggaaa ggtcattttt cctgaatatg ctcacatcat ataaagaaat acagataaag
     9661 ttattatctg cttgtggtgg tgaatgcact gaccggctat aaggaaaggc caaacaagaa
     9721 cacggttgca aaaaccgtgc ccttaaatat tggatctcta ttcagaacac tttcttaaat
     9781 ggtttcactg aaacgtgttc atagacacct gccgctacgt acgggtcagc atcggcccag
     9841 gcctgagctg cttccagcga ttcaaattca gcaataacgg ttgagccagt aaatcccgca
     9901 gcccctggat cgttactgtc taccgctggc attggaccag ctgtcaacaa acgaccttca
     9961 tcatgcagta actgtaaacg tgctaaatgt gccggacgaa cggaaaggcg tttttcgagg
    10021 ctatcagctt tgtcttgagc gtaaataaca tacaacacgg gcacaactcc ttgttcggga
    10081 aagttgtaaa gtacgttatt tgaaagggcg aagatctgca acggaaagat gatgtctttg
    10141 ttaagaccat gcataaagta aggttaatta cgccaaaaat gacattttca ctgatcctga
    10201 tcgtctttcc ttattgaata tgattgctat ttgcatttaa aatcgagacc tggtttttct
    10261 actgaaatga ttatgacttc aatgaccctt gatttacctc gccgcttccc ctggccgacg
    10321 ttactttcgg tctgcattca tggtgctgtt gtggcgggtc tgctctatac ctcggtacat
    10381 caggttattg aactacctgc gcctgcgcag ccaatttctg tcacgatggt tgcgcctgct
    10441 gatctcgaac cgccacaagc cgttcagccg ccaccggagc cggtggtaga gccagaaccg
    10501 gaacctgagc cgatccccga accgccaaaa gaagcaccgg tggtcattga aaagccgaag
    10561 ccgaaaccta agccaaaacc gaagccggtg aaaaaggtac aggagcagcc aaaacgcgat
    10621 gtcaaacccg tagagtcgcg tccggcatca ccgtttgaaa atacggcacc ggcacgcccg
    10681 acatcaagta cagcaacggc tgcaaccagc aagccggtta ccagtgtggc ttcaggacca
    10741 cgcgcattaa gccgtaatca gccgcagtat ccggcacgag cacaggcatt gcgcattgaa
    10801 gggcaggtta aagttaaatt tgatgtcacg ccggatggtc gcgtggataa cgtacaaatc
    10861 ctctcagcca agcctgcgaa catgtttgag cgtgaggtga aaaatgcgat gcgcagatgg
    10921 cgttatgagc cgggtaagcc aggcagtggg attgtggtga acatcctgtt taaaattaac
    10981 ggcaccaccg aaattcagta agcataaagt caaaagcctc cgaccggagg cttttgatta
    11041 ttactcaaca ggtaaggcgc gaggttttcc ttcaggatca accgcgacat acttaaataa
    11101 tgcttctgtc gctttatagc gttgcccgat tggttcagac gctacttttt tcacccacac
    11161 ctcaatatta atgctgaccg atgtcgtccc tttctggaca cagcgtgcat agcagcacac
    11221 cacatcgccg accgcaaccg gccgtaagaa agtcattcct tcaacccgca cagtcactac
    11281 gcgaccgtgg gcaatttctt ttgccagaat agcgccgcca atatccattt gtgacattaa
    11341 ccaaccacca aagatgtcac cattggcatt ggtatcggcg ggcatggcta aagtacgtaa
    11401 aacaagatcg ccctgaggga cgttatgtgt tgtagacatg gtaaaaccga cttaaaagga
    11461 aaatcacagg cgcgatgcta ctatgatttt cctgaggaga acagaggaga tacagcgcca
    11521 gccccgaagg actggcagtc tggttaggat ttatcttcct gcggcatgtg gcggtagata
    11581 tagataccgc ttaacaatgt gaagattaag gtaagggcgg tcaggccaaa gactttaaag
    11641 ttgacccaaa tattttgcgg cagccagaat gcgatgtaga tgtttgccag accgcaaagg
    11701 ataaagaaaa cagcccaggc cagattcagc ttcgaccata ccggttgcgg cagcgtgagt
    11761 tctttaccca gcatccgctg aattagcggc tttttcatca cccattggct gactaacagg
    11821 gctcccgcaa acagagcata aatgactgta accttccatt taataaactc atcattgtgg
    11881 aagaacagcg tcaagccacc gaagacgacc accagaacaa aagtgatcag ggccatcttc
    11941 tcaaccttac gaaagcgaac ccagctatag ataagcacaa tcgccgtggc gacgatcagc
    12001 gccgcagtag ccgcatagat atcataaatc ttgtaaaacg cgaaaaagac aaccagcggt
    12061 aaaaaatcaa gaaactgctt cattttacga ttccgtaatc aaatgcttaa ggttattggc
    12121 gaatcaacat atacagacgg aacagataga tgagcaatac ggctgaaatc aggttgctca
    12181 aggtgttcgc cagtacggca ccaatttccg gggttaatgc ggcaaaagaa gaggcaaaaa
    12241 gcagcaacag tgtttttgcc agcaaccagc tcagtactgc gggtgccacc agacgcatat
    12301 tcgcccaagt cagccgcata ctgctacgca tcgaggcaaa aatgcccatt ttgtcctgta
    12361 ccaacatcac cggtgccagg gccagtaaaa tggccatgat aattcccgga acgaccacta
    12421 gcatgatgcc aatctgtact aaaagggtag tcagaaaaat cagaataaat aacttgggca
    12481 atatcggcgc actggcacca atagctcgca gcgcactgac tctctgaccc gcagacacca
    12541 gctggataat taatattacg cctccggcga gaatggcgtt accgattaat cctgaaaaag
    12601 tggacgccgc tgaagcctgc agcaaaattt gttgctgttc cggtgacata ttctgaacca
    12661 ggtcgaacaa cccactactg ccgctaacgg gaacgccgtc attgagctgc gcaagctgtg
    12721 catcactggg tgagaaaaca tgccctaaca ccactgtgat aaacgcacat agcaacgata
    12781 ccaacagaat ggtcataaat tgattacgga agaaatttcc ggtgtcacgg tatacggact
    12841 gcgccgtgat agacatgcac tctccttgag ttttatgcag gtgttaatta gcgggcaatt
    12901 gtaccctggt taagcctcgg gtggcagcat caggtcttgt atggaaaagc atatctttgt
    12961 aaagcggagg taattgctgg ccttcaggcg tgggcaaacc gcggcaagtg gcgatcgagt
    13021 taatatttgc gtagcgaaaa tatttaaaaa ttgatttaaa tcacgttaac c
//
LOCUS       DAPGOF010000133        13051 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.134, whole genome shotgun sequence.
ACCESSION   DAPGOF010000133 DAPGOF010000000
VERSION     DAPGOF010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13051
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.134"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(424..1515)
                     /gene="ychF"
                     /locus_tag="RHV13_003441"
     CDS             complement(424..1515)
                     /gene="ychF"
                     /locus_tag="RHV13_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDV1242943.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1632..2216)
                     /gene="pth"
                     /locus_tag="RHV13_003442"
     CDS             complement(1632..2216)
                     /gene="pth"
                     /locus_tag="RHV13_003442"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDV1242944.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2494..2772
                     /gene="ychH"
                     /locus_tag="RHV13_003443"
     CDS             2494..2772
                     /gene="ychH"
                     /locus_tag="RHV13_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HDV1242945.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMSQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2827..4506)
                     /gene="dauA"
                     /locus_tag="RHV13_003444"
     CDS             complement(2827..4506)
                     /gene="dauA"
                     /locus_tag="RHV13_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HDV1242946.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4631..5578)
                     /gene="prs"
                     /locus_tag="RHV13_003445"
     CDS             complement(4631..5578)
                     /gene="prs"
                     /locus_tag="RHV13_003445"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDV1242947.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5729..6580)
                     /gene="ispE"
                     /locus_tag="RHV13_003446"
     CDS             complement(5729..6580)
                     /gene="ispE"
                     /locus_tag="RHV13_003446"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDV1242948.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6580..7203)
                     /gene="lolB"
                     /locus_tag="RHV13_003447"
     CDS             complement(6580..7203)
                     /gene="lolB"
                     /locus_tag="RHV13_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HDV1242949.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7417..8673
                     /gene="hemA"
                     /locus_tag="RHV13_003448"
     CDS             7417..8673
                     /gene="hemA"
                     /locus_tag="RHV13_003448"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HDV1242950.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEQVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8715..9797
                     /gene="prfA"
                     /locus_tag="RHV13_003449"
     CDS             8715..9797
                     /gene="prfA"
                     /locus_tag="RHV13_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDV1242951.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9797..10630
                     /gene="prmC"
                     /locus_tag="RHV13_003450"
     CDS             9797..10630
                     /gene="prmC"
                     /locus_tag="RHV13_003450"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDV1242952.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10627..11019
                     /locus_tag="RHV13_003451"
     CDS             10627..11019
                     /locus_tag="RHV13_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HDV1242953.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIVLMAKAHILPFSGQAQWLTEKLFGVIIYTVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11023..11832
                     /locus_tag="RHV13_003452"
     CDS             11023..11832
                     /locus_tag="RHV13_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HDV1242954.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            11868..12722
                     /gene="kdsA"
                     /locus_tag="RHV13_003453"
     CDS             11868..12722
                     /gene="kdsA"
                     /locus_tag="RHV13_003453"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HDV1242955.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(12870..12977)
                     /locus_tag="RHV13_003454"
     CDS             complement(12870..12977)
                     /locus_tag="RHV13_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDV1242956.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
BASE COUNT         3233 a         3298 c         3394 g         3126 t
ORIGIN      
        1 tttttgatca atgatttttc tggcatatcg ttattatcgt gctattggtt atcattttca
       61 tcttgccagg atcgcacgta atcacgttat tcggggactt tatctgcggg atattaccct
      121 tcaggtaagt ccttttcttg ccagcatatt ggagcgtgat caatattgaa cagctgtgaa
      181 gggggctgat gtgtagtgga acaaaaactg acgttatatc actgctaatg agatatccgt
      241 catctgaaca cttatgtctt taattattct ggtggttcac tgtaggcaat aagcacaaaa
      301 gtgtaggatg ttacaagaat gattaggact cggtgaaatg aaaaatccac gcaattgcgt
      361 ggattttata tacttttgcg ctcttcatga gatttagcga aacctcatga gacaacaaat
      421 taattagacg ttgaaaagga agttcatcac atcgccatct ttaacgatgt aatctttacc
      481 ttctgcacgc attttgcctg cttctttcgc gccttgttca cctttgtaag tgatgaaatc
      541 ttcaaacgag atggtttgtg cacggataaa gcctttttca aaatcagtat ggattttgcc
      601 cgctgcctgc ggcgcggttg ctccaaccgg aatggtccat gcacgcactt ctttcacccc
      661 agcggtgaag taagtttgca ggttcagcag cttataaccg gcacggatca cacggttcag
      721 gcccggctct tccagcccaa gctcctgcat aaactcgtca cgttcttcgt cgtccagttc
      781 ggcaatgtct gcttcaacag cagcacaaac cggaaccaca acagaacctt ctttcgccgc
      841 gatttcacgc acctggtcaa gatatgggtt gttttcaaaa ccgtcttcgt tgacgttggc
      901 gatgtacatt gttggtttca gcgtcaggaa gctcaggtaa cgaatagccg ctttctcttc
      961 agcgcttaaa tccagcgcgc gcagcatacc tgcgttttcc agctggggca ggcatttttc
     1021 caggaccgcc agctcagctt tcgcgtcttt atcgccacct ttggctttct tctgtacgcg
     1081 atgaatcgca cgttcgcagg tgtcgaggtc tgccagcgcc agttcggtgt tgataacttc
     1141 aatatcgtca gccgggttaa ctttgccgga aacgtgaatg atgttgtcat tttcaaagca
     1201 gcgaacaacg tgaccgatcg cttcggtttc acggatgttg gtcaggaact ggttacccag
     1261 accttcgcct ttcgatgcgc cttttaccag accggcgata tcgacaaatt ccatggtcgt
     1321 gggaagcgta cgctgcggtt ttacgatttc agccagttga tccaggcgag gatcaggcat
     1381 tggtacgacg cctgtgttcg gctcaatagt gcagaatgga aagttggccg cttcaatacc
     1441 ggctttggtc agcgcgttga acagggtaga tttcccgacg ttgggcaaac cgacgatacc
     1501 gcatttgaat cccatgattt aaatcacctt aatatcttaa taatcaacct gttattgcta
     1561 acagattgca gaaatggaaa taactttgcc tattatacac ggcactcggc aaaaatgccg
     1621 cagacaacga cttattgcgc tttaaaggcg tgcaatcggt tcgttgcttt ggtcaagcca
     1681 tctgtaaacc acatttcagt acaacgcgcc gcttcgtcga tggcttcatc aattaacttc
     1741 tgttcactaa caggcggttt gcctaacaca aaaccaacaa ctttattttt atcgcccgga
     1801 tgaccgattc cgatgcgtaa acggtgaaag ttagggttat tacccaattt actgatgatg
     1861 tctttcagtc cattgtgacc accatggcca ccgcccaact taaatttggc gacgccagga
     1921 ggcagatcca gttcgtcgtg ggccaccaga atttcgtccg gattaatgcg gaaaaaactg
     1981 gccatcgccg caacggcttt gccgctgaga ttcataaatg tagtcgggac taacaggcgg
     2041 acatcttcgc ctccaagggt gactcgcgaa gtataaccaa agaatttagc ctcttcgcgc
     2101 agcggagcgc gcaaacgctc tgccagtaag tcaacgaacc aggcaccagc attatgtcgc
     2161 gttgcggcgt attcagcacc ggggttcgcc aggccgacaa tcaatttaat cgtcacgttt
     2221 ttttgtcctg agtgtgtaca taactggcgc gtagtttact ggttgcggcc ccgcttgaca
     2281 aaaaactgcg tatcaaatgc agataacgta ataattgcct gagtggacta ttagaaagtc
     2341 agggtgttca ggcgtttatt tgtaaagttt tgttgaaata agggttgtaa ttgtgatcac
     2401 gcccgcacat aacccacagg gtgttgtcta tactttacac ataaggaaga ggggtattcc
     2461 ctgttacaac ccagaaagtt ccggaggtga catatgaaac gcaaaaacgc ttcgttactc
     2521 ggtaacgtgc tcatggggtt gggtctggtg gtaatggtgg tcggcgtggg gtattcaatc
     2581 ctcaaccagt taccacagtt taatatgtcc cagtatttcg cacatggtgc agtgctaagt
     2641 attttcgtcg gtgccattct ctggctggcg ggtgcccgtg ttggcgggca tgaacaggtg
     2701 tgcgaccgtt actggtgggt tcgccactat gacaaacgtt gccgccgtag cgataatcgc
     2761 cgtcatagct aacaaataat gcagtttgct gaccagtcag tttgcgctga ctggtcaatc
     2821 tcgtacttat aaatccgcca tcgccgcgcg acgattcggg aagaacgcga ggcgtcccgg
     2881 gatcggttga atgcctgcgc gagccatagt gcgcagtggc tggaattcca cgttgcacac
     2941 gcgcagttca catccttcgg gcagacgctt cacaaaacgc tggaacgcat caagaccacc
     3001 agcatcaagt accggaacgg catcccactt cagaatcaca atccgtttgc cttcaagacg
     3061 tgactccagg tccgtgaata agccttcagc agcagcaaaa aacagcgggc caataacgcg
     3121 cagaaccagg acatcgtctg gaacatctac gactaccggt gccaggcgag tcatacgtgc
     3181 gatacgacgc ataaacagca gcgatgccag cacgatcccc acgctgatgg caataaccat
     3241 atcaaacagc acggtcagcg acatgcacag cagcatgacg atgatgtcat ctttcggcgc
     3301 atgacgcagc aagtcgacca ctttatgcgc ttcactcatg ttccacgcca ccatcaacag
     3361 cagggctgcc atagcggaaa gcggcagcca ggagagcagc ggtgccagta ccagcagggc
     3421 aagaataacc agaatagagt ggatcaccgc cgagataggg gaagttgccc cggcacggac
     3481 gttagcggca gaacgcgcga tggcagctgt agcggtaata ccaccaaaga acggagcgat
     3541 gatattcccc agtccctgtc caaccagttc gctattcgcc ttgtgtttcg tcccggtcat
     3601 accatccagc accacggcgc agagcagaga ttcgattgcg ccgagcattg ccattgagaa
     3661 tgccgcaggc agcagtgtgc gaatagaatc ccaggttagc gtgaattctg aattaggcag
     3721 atcccacggc agcaccagtt gcggcagcag ttgcggaata ccgttaccct gagaaccatc
     3781 ggccaggacg tagtggaatt gcgaaccgat ggtagcaaca tgtccgccga gcaggttaac
     3841 aatccccatc accgcgcaac cagccagcaa ggccggaagg tggccaggta aacgaatgcc
     3901 cagacgcggc cagaaaacca gaatgccaag cgtcacaatg ccaatggcag catcacccac
     3961 attaatggtc ggcagcgcca taaataatgc accgactttt tgtagataat gttccgggac
     4021 atgggccatt tgcagaccga gaaaatcttt aatctgcatg gtaccgatgg tgatcccgat
     4081 acccgaggtg aaacctaagg tgacggaaac cggaatatac tcaatcaggc gaccaaagcg
     4141 tgccagaccc atcagaatca aaaagatccc cgacagcaag gtcgcaacca gcagtcctgc
     4201 cagcccaaac tgttgcgaaa cgggatagag aattaccaca aatgccgcag tcggaccgga
     4261 aacgctaaag cgtgacccac ccgtcagagc aatgacaatc cccgcaacag ctgcggtata
     4321 taaaccgtac tggggtgcca caccactacc aatagccaac gccatcgcca gcgggatagc
     4381 aataatcccg acggttatcc cggcaatcag gtcacgggta aaccgtgcgg cagtatattt
     4441 ttctttccag caagcgtcga tcagagcgcg gaaaggcatc acatgtgagg aaaatatttt
     4501 gttcacaata atgtttcatc cgtgagcgca tcatctgtca actaaatggc aggtgaagga
     4561 ggcataggtc atacaaatgg atattacaga caaaaaaacc cgccgcagcg ggtctttgag
     4621 ccgggttcga ttagtgttcg aacatggcag agatcgattc ttcgttgctg atacgacgaa
     4681 tcgcttcggc cagcatacct gacagggtca gagtacgcac gttcggcagt gatttgattt
     4741 catcgctcag cggaatggta tcgcagacaa cgacttcatc aattacagag ttacgcaggt
     4801 tgttcgccgc gttgccagag aagatcgggt gagtcgcgta cgcaaataca cgtttagcac
     4861 cacgttcttt cagagcttca gcagctttac acagcgtacc gccagtgtcg atcatatcat
     4921 cgaccagtac gcagtcacga cctgcaacgt caccgatgat atgcatcacc tgggaaacgt
     4981 tcgcacgcgg acgacgtttg tcgatgattg ccatatcggt atcgttcagc agcttagcga
     5041 tagcgcgggc acgcacaacg ccgccgatgt ccggagaaac cacaattggg ttatccagat
     5101 tcagctgcag catgtcttcc agcaggatcg ggctaccaaa tacgttatca accggaacgt
     5161 cgaagaaacc ctgaatctgt tcagcgtgca gatccactgt cagcacacgg tcaacaccga
     5221 cgctggagag gaagtctgca accactttcg cagtgattgg tacacgagcg gaacggacgc
     5281 gacggtcctg gcgcgcatag ccaaagtagg ggataacagc ggtgatacga cctgcggaag
     5341 cacgacgcag ggcatcaacc ataacgacta attccatcag gttgtcgtta gtaggggcac
     5401 aagtggactg gatgatgaaa atatcaccac cgcgtacatt ttcgttgatt tgtacactga
     5461 cttcgccgtc gctaaagcga cctacagcgg cgtcgccgag tgaagtgtac aggcggttgg
     5521 caatacgttg tgctagttcc ggggtggcgt taccagcaaa aagcttcata tcaggcacga
     5581 gaagaacctc aggcatgcgt ccattggtgg aaagaatctg ccgaaaactg tgcgggccag
     5641 gcatgatcct ttccaggcgg tgtattaaag agcgcgatgc aacgtctgga acaaggtgac
     5701 gttgtcaccg aaactcagct tgcccggctt aaagcatggc tctgtgcaat ggggaaagat
     5761 taacaccttt cgccacaaag ccattgagcc attccggggc ttgctctagc acctggcggg
     5821 cttcagactc tgtatcaaat tcagcaaaga cacaggcccc tgtcccagtc aggcgcgacg
     5881 gggcgtattc taacagccag gaaagcaccg catcaacctc gcgaaaacgt tttcttgcga
     5941 taacctcgca atcattgctg aattcacatt ttagcaacgt ttctattgac ctttttggcg
     6001 tattgcgcgg gagttcagga tctttaaaaa tcactggtgt cggaatactt acaccagggt
     6061 gcgccaccag ataccacttc tctggcggat ccaccggcgt tagtatttca ccaacgcctt
     6121 cggcaaacgc ggcatgcccc cgaacaaaga caggaacatc tgcgcccagc gtcagcccca
     6181 tttccgccag ctcatccatg cttagcccgc attgccagag atgatttaat gccaccagga
     6241 ccgtcgcggc attggatgaa ccaccgccga gaccgccgcc catcggcaaa cgcttgtcaa
     6301 tgctgatatt cgcaccgctt cccgtcggaa gacgcccgct gtctgccgca gttttcatca
     6361 acaaccgcgc tgcgcgaacg atcaggttat cttcatgttc cacgccttca acgggcgtta
     6421 acagacgaat atccccatcg tcacgaagct caatgctgat ggtgtcgccg taatcaagaa
     6481 actgaaacag cgtttgcagc gtgtggtaac catccgcacg ctgaccggta atgtataaaa
     6541 acagattaag ttttgccgga gagggccact gtgtccgcat tatttcacta tccagttatc
     6601 catttttaac ttgatgcgtt gaccaccgtc ggtgagttcc atattggctg gcatcgcagg
     6661 ttgcgttttg gtgtcataac caccataaac aaccttccag tttttgccat tctggctgta
     6721 ggtaatttcg ctcaggcggt actggtcgtc cagtttgtag tcggttgcat cacccggtaa
     6781 acctaaaatc cactggcgca agctgttgag cggaattggc attccggtca atttgccaat
     6841 catctcttcg gcgtcatcgg cggtataacg ctgaccttta ttgtcgacta actgcacgtt
     6901 acccggttga gcattcagct ccagttccgt gctgcccaat gggttagtga gcagcagacg
     6961 gtagcgatcc tggccggttt gctgccagaa aaagcgggcg tacacttttt gttggtcaga
     7021 aatataagcg aacgcgccgc gagtctgata ctgattaaga ttgcgcacgt cttgctgatg
     7081 ctgacgccat tgtggcgaat ccgggctttt gccaggacct ttgggcgtgg taacggaaca
     7141 ggcagtgagc acaagagcag ccagcggtag caggcggata agacgaaaat cgggcagggg
     7201 catagtgatg acaagtcctt gagatacgtt gcagttataa cccttaatgc tagcgttacc
     7261 gtccgctatc gtctatgttc aagttgtctt aattgccaga atctaacggc tttcggcaat
     7321 tactccaaaa gggggcgctc tcttttattg atcttacgca tcctgtatga tgcaagcaga
     7381 ctaaccctat caacgttggt attatttccc gcagacatga cccttttagc actcggtatc
     7441 aaccataaaa cggcacctgt atcgctgcga gaacgtgtat cgttttcgcc ggataagctc
     7501 gatcaggcgc ttgacagcct gcttgcgcag ccgatggtgc agggcggcgt ggtgctgtcg
     7561 acgtgcaacc gcacggaact ttatcttagc gttgaagagc aggataacct gcaagaggcg
     7621 ttaatccgct ggctttgcga ttatcacaat cttaatgaag aagatctgcg taaaagcctc
     7681 tactggcatc aggataacga cgcggttagc catttaatgc gtgttgccag cggcctggat
     7741 tcactggttc ttggggagcc gcagatcctc ggtcaggtta aaaaagcgtt tgccgattcg
     7801 caaaaaggtc atatgaaggc cagcgaactg gaacgcatgt tccagaaatc tttctctgta
     7861 gcgaaacgcg ttcgcactga aacagatatc ggtgccagtg ctgtgtctgt cgcttttgcg
     7921 gcttgtacgc tggcgcggca gatctttgaa tcgctctcta cggtcacagt gttgctggta
     7981 ggcgcgggcg aaaccatcga gctggtagcg cgtcatctgc gcgaacataa agtacagaag
     8041 atgattatcg ccaaccgcac tcgcgaacgt gcccaaattc tggcagatga agttggcgcg
     8101 gaagtgattg ccctgagtga gatcgacgaa cgtctgcgcg aagccgatat catcatcagt
     8161 tccaccgcca gcccgttacc gattatcggg aaaggcatgg tggagcgcgc attaaaaagc
     8221 cgtcgcaacc aaccaatgct gttggtggat attgccgttc cgcgcgatgt tgagccggaa
     8281 gttggcaaac tggcgaatgc ttatctttat agcgttgatg atctgcaaag catcatttcg
     8341 cacaacctgg cgcagcgtaa agccgcagcg gttgaggcgg aaactattgt cgcccaggaa
     8401 accagcgaat ttatggcgtg gctgcgtgca caaagcgcca gcgagaccat tcgcgagtat
     8461 cgcagccagg cagagcaagt tcgcgatgag ttaaccgcca aagcgttagc ggcccttgag
     8521 cagggcggcg acgcgcaagc cattatgcag gatctggcat ggaaactgac taaccgcttg
     8581 atccatgcgc caacgaaatc acttcaacag gccgcccgtg acggggataa cgaacgcctg
     8641 aatattctgc gcgacagcct cgggctggag tagcagtaca tcattttctt tttttacagg
     8701 gtgcatttac gcctatgaag ccttctatcg ttgccaaact ggaagccctg catgaacgcc
     8761 atgaagaagt tcaggcgttg ctgggtgacg cgcaaactat cgccgaccag gaacgttttc
     8821 gcgcattatc acgcgaatat gcgcagttaa gtgatgtttc gcgctgtttt accgactggc
     8881 aacaggttca ggaagatatc gaaaccgcac agatgatgct cgacgatcct gaaatgcgtg
     8941 agatggcgca ggatgaactg cgcgaagcta aagaaaaaag cgagcaactg gaacagcaat
     9001 tacaggttct gttactgcca aaagatcctg atgacgaacg taacgccttc ctcgaagtcc
     9061 gagccggaac cggcggcgac gaagcggcgc tgttcgcggg cgatctgttc cgtatgtaca
     9121 gccgttacgc cgaagcccgc cgctggcggg tagaaatcat gagcgccagc gagggtgaac
     9181 atggtggtta taaagagatc atcgccaaaa ttagcggtga tggtgtgtat ggtcgtctga
     9241 aatttgaatc tggcggtcat cgcgtgcagc gtgttcctgc tacggaatcg cagggtcgta
     9301 ttcatacttc tgcttgtacc gttgcggtaa tgccagaact gcctgacgca gaactgccgg
     9361 acatcaaccc agcagattta cgcattgata ctttccgctc gtcaggggcg ggtggtcagc
     9421 acgttaacac caccgattcg gcaattcgta ttactcactt gccgaccggg attgttgttg
     9481 aatgtcagga cgaacgttca caacataaaa acaaagcgaa ggcgctttca gtgctcggtg
     9541 ctcgcatcca cgctgctgaa atggcaaaac ggcaacaggc cgaagcgtct acccgtcgta
     9601 acctgctggg gagtggcgat cgcagcgacc gtaaccgtac ttacaacttc ccgcaggggc
     9661 gcgttaccga tcaccgcatc aacctgacgc tctaccgcct ggatgaagtg atggaaggta
     9721 agctggatat gctgattgaa ccgattatcc aggaacatca ggccgaccaa ctggcggcgt
     9781 tgtccgagca ggaataatgg aatatcaaca ctggttacgt gaagcaataa gccaacttca
     9841 ggcgagcgaa agcccgcggc gtgatgctga aatcctgctg gagcatgtta ccggcagagg
     9901 gcgtactttt attctcgcct ttggtgaaac gcagctgact gacgaacaat gtcagcaact
     9961 tgatgcgcta ctgacacgtc gtcgcgatgg tgaacccatt gctcatttaa ccggggtgcg
    10021 agaattctgg tcgttgccgt tatttgtttc gccagcgacc ttaattccgc gcccggatac
    10081 ggagtgtctg gtggagcagg cactggcgcg gttgcctgaa caaccttgcc gtattctcga
    10141 tctcgggacg ggtaccgggg cgattgcgct tgcgctggct agcgagcgcc cggactgcga
    10201 aattatcgct gtagatcgta tgcctgatgc tgtctccctg gcacaacgta atgcccagca
    10261 tctggcgatc aaaaatatcc acattctgca aagcgactgg tttagcgcgc tagccgggca
    10321 gcagtttgcg atgattgtca gcaatccgcc gtatattgac gagcaggacc ctcatcttca
    10381 acaaggcgat gtccgctttg agccgctcac tgcgctggtt gcggcagaca gtggaatggc
    10441 agacatcgtg catatcatcg aacagtcgcg taacgcgctg gtatccggcg gctttctgct
    10501 tctggaacat ggctggcagc agggcgaagc ggtgcgacaa gcatttatcc ttgcggggta
    10561 tcatgacgtc gaaacctgcc gtgactatgg tgataatgag cgcgtaacgc tcggccgcta
    10621 ttatcaatga caagtttttc tacactgctt agtgttcatc ttattagtat cgcgctttct
    10681 gttgggctat taaccttacg tttctggcta cgttatcaga agcatcctca ggcatttgcg
    10741 cgctggacgc gcattgtgcc gccggttgtc gatacggtgt tattgttaag cggcattgtg
    10801 ttgatggcta aagcgcacat cctgccattt tccgggcagg cacagtggct gactgaaaag
    10861 ctgtttggag ttatcattta taccgttttg ggttttattg cactcgatta tcgtcgtatg
    10921 cacagtcagc aggcgcgcat tattgccttc ccgctggcgt tggtggtgct gtacatcatc
    10981 attaaactcg ccaccacaaa agtaccgtta ctggggtaag tcatgagatc gttagctgat
    11041 ttcgaattta ataaagcgcc attgtgcgaa ggcatgatcc tggcttgcga agcaatccgc
    11101 cgcgattttc cctcgcaaga tgtttacgac gaactggagc gtctcgttag tctggcgaag
    11161 gaagaaatca gccagcttct gcctttagaa gagcagctgg aaaaactgat cgcgctgttt
    11221 tacggcgact ggggatttaa agcctcacgc ggcgtttatc gtctttccga tgcattatgg
    11281 ctggaccagg tgttaaagaa tcgacagggc agtgcggtat cattaggtgc ggttttatta
    11341 tgggtcgcga atcgtctcga tttgccgctg ctgccggtga ttttccctac gcagctgata
    11401 ttgcgcattg aatgtccgga tggcgaaatt tggctgatta atccttttaa cggtgaatcg
    11461 ttaagcgaac atatgctgga cgtatggtta aagggaaata tcagcccgtc ggcggaactg
    11521 ttttatgaag accttgatga agctgataac attgaggtaa tccgcaaatt gctggataca
    11581 ctcaaagcct cgttgatgga agaaaatcag atggagctgg cgttacgcac cagcgaagct
    11641 ttattacaat tcaaccctga agatccctat gaaattcgcg atcgcgggtt gatttatgcg
    11701 caactggatt gcgaacacgt tgcgttgaac gatttaagtt atttcgttga acaatgtccg
    11761 gaagacccga tcagcgaaat gatccgtgcg caaataaata acatcgcgca taagcatatt
    11821 gtgctgcatt aattaatcga cattttactc aagattaagg cgatcctatg aaacaaaaag
    11881 tggttagcat tggcgacatc aacgtagcaa atgacctgcc gttcgtactg tttggcggta
    11941 tgaacgtgct ggaatctcgc gatctggcga tgcgcatttg cgagcactac gtaaccgtga
    12001 cccagaaact gggtatccct tacgtgttca aagcctcttt tgacaaagcc aaccgctcct
    12061 ccatccactc ttatcgtgga ccgggcctgg aagaagggat gaaaatcttc caggagttga
    12121 agcagacttt tggcgtgaaa attatcaccg acgttcacga accaagtcag gcacagcccg
    12181 ttgctgatgt cgtggatgtg atccagttgc cggcgtttct tgctcgccag actgacctgg
    12241 ttgaagccat ggcgaaaacc ggtgcggtaa ttaacgtcaa gaaaccacag tttgtcagcc
    12301 cgggacagat gggtaatatc gttgataaat tcaaagaagg cggcaacgaa aaagtgattc
    12361 tttgcgatcg tggtgctaac ttcggctatg acaacctggt tgtcgatatg ctgggcttca
    12421 gcattatgaa gaaagtgtct ggtaactcgc cggtgatttt cgacgtgacc cacgcattgc
    12481 aatgccgcga tccttttggc gcagcttccg gcggtcgtcg tgctcaggtg gctgagctgg
    12541 cacgagccgg tatggcggta ggtctggcgg ggctgtttat tgaagcgcat ccggatccgg
    12601 aacatgcgaa atgtgatggt ccatccgcgc tgccgctggc taaactggaa ccgttcctca
    12661 agcagatgaa agcgattgat gatctggtga aaggtttcga agaactggat accagcaagt
    12721 aatctttttt gcttgaaaaa taaagtctta gcgttctgcg ttaagacttt tttcatgggt
    12781 gccggataca aaaaaggccg caggctgtta ccctgcggcc ggtttcgggc gcatattgcc
    12841 atcacggcag cctgacgccc gttttcgcct tacttccggt tacgccacca gccgacaatc
    12901 gctgcggtaa taattcccgc caggatcggt gctgccaggt cgtgccagaa aatcatggca
    12961 aactgcgcga gcgtcatata gccgccttgt tgtaatgaca acattttgcg gctattcttg
    13021 aattgttgat ggttcaagat tagcccccgt t
//
LOCUS       DAPGOF010000134        13048 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.135, whole genome shotgun sequence.
ACCESSION   DAPGOF010000134 DAPGOF010000000
VERSION     DAPGOF010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13048
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.135"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            10..1032
                     /locus_tag="RHV13_003455"
     CDS             10..1032
                     /locus_tag="RHV13_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HDV1242957.1"
                     /translation="MSLTRRRFTQILASTLFLHHLPSFAQSVKFWASLTLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETWISQARQVSEQTQIPWLLLNGKLEQSAEQLTTLGKTLG
                     EEHRAAEQANLASRFVGEAQAFATSPAANLRFYAARGPRGLETGLKGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            1032..2012
                     /locus_tag="RHV13_003456"
     CDS             1032..2012
                     /locus_tag="RHV13_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDV1242958.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVPV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACTA
                     ILWGLSIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLHDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWVGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            2009..2767
                     /locus_tag="RHV13_003457"
     CDS             2009..2767
                     /locus_tag="RHV13_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV1242959.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEVLLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLAARRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISH"
     gene            2777..3589
                     /locus_tag="RHV13_003458"
     CDS             2777..3589
                     /locus_tag="RHV13_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDV1242960.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTIYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMHQAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYRWYQQQDARALAPASRDWALIWWDSVPQEALR"
     gene            3586..4440
                     /locus_tag="RHV13_003459"
     CDS             3586..4440
                     /locus_tag="RHV13_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HDV1242961.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQHGYIEFFLRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAIQNVLEW
                     SCGVSDYLDQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQDWSGAIDQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSRAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPAASI"
     gene            4466..6436
                     /locus_tag="RHV13_003460"
     CDS             4466..6436
                     /locus_tag="RHV13_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDV1242962.1"
                     /translation="MRLKKHYLCTALSLAFTQQAVAAQESDSLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLQGPNQMGGAINITTQKPTKPLEASLGYRQG
                     WSRSQDNAYDMHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMINSSA
                     DDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNSGQKSRYWQWPEYDKESFYY
                     QGTTQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQLAAD
                     VRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVAGISY
                     DWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFVNEDTLALSYYDRTRFPTLK
                     ERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDAILSH
                     NIDADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKITDLPT
                     QTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHGFSVN
                     ASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(6486..6740)
                     /locus_tag="RHV13_003461"
     CDS             complement(6486..6740)
                     /locus_tag="RHV13_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDV1242963.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            6941..7675
                     /locus_tag="RHV13_003462"
     CDS             6941..7675
                     /locus_tag="RHV13_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HDV1242964.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRISWNGGQLISKIL
                     VITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AGLHEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            complement(7677..8288)
                     /gene="emtA"
                     /locus_tag="RHV13_003463"
     CDS             complement(7677..8288)
                     /gene="emtA"
                     /locus_tag="RHV13_003463"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HDV1242965.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            8388..9302
                     /gene="ldcA"
                     /locus_tag="RHV13_003464"
     CDS             8388..9302
                     /gene="ldcA"
                     /locus_tag="RHV13_003464"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HDV1242966.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNKTFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            9397..11133
                     /locus_tag="RHV13_003465"
     CDS             9397..11133
                     /locus_tag="RHV13_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HDV1242967.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(11235..11387)
                     /locus_tag="RHV13_003466"
     CDS             complement(11235..11387)
                     /locus_tag="RHV13_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001599728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242968.1"
                     /translation="MVDGDTGNAGLVILHLLQSEEAITLTFLSRCFELIGQSLQWFVI
                     HNLTFF"
     gene            11783..>13048
                     /locus_tag="RHV13_003467"
     CDS             11783..>13048
                     /locus_tag="RHV13_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242969.1"
                     /translation="MASIKDIRPFAPGIPPSSVNPGVRRPPGQYFLPVELKNEILRNM
                     KYIGPQIFSWGDDHRQIIDLSTAGKVKIHMVDNTIIMLERLNPNPQHNEFMMVAEWCQ
                     WAMERDGLQENLQKNLYEILEENQQNKQSEIPQEDLKESLEEIKVNILEENLPASRIE
                     NRAEALRRMKECLITRRSMLNLSNLGLTSLPENLPPHLIEFYCSKNVLTALPKVMPKW
                     LLVLDCTDNVLILLPKVQPSKLMVLNCYDNCIIWLPELSTNLRVINCSANFLQFLPPS
                     MPQYLYKLSCAGNNIHSIPDEMLENLTRLKVFDCSSNDLISSPRLPPKLIIYYCGENK
                     FKTVQVPQPQSLKVFDCNGNPWDKDNLPTLLKAVEGLKNQEGLVDLLDFLRKEGQFDL
                     EGLEELEDLEDLMVLEHLEDPVLLERVKVQ"
BASE COUNT         3122 a         3383 c         3342 g         3201 t
ORIGIN      
        1 atataactta tgtcactaac cagacggagg tttacacaga ttcttgcgtc gacgttgttc
       61 ctgcatcatc tgccttcttt tgcacaatca gtcaaattct gggcctcact gacgcttccc
      121 gaagcccaaa acattacacg catcgtcagc gcaggtgcgc ccgccgattt gctattgctg
      181 gctgtcgcgc cagaaaaaat ggtcggtttt tcctcttttg attttgcccg tcaggcatta
      241 attccattgc cagagcacat tcgccagtta cccaggctgg gacgactcgc cgggcgcgcc
      301 agcacactct cgctggaagg gcttatggcg ttacatcccg atttggtcgt tgattgcggc
      361 aatacggatg aaacctggat ctcccaggca cggcaggtta gcgaacagac acaaataccc
      421 tggttattgc ttaacgggaa actggagcaa tcagcagaac agttaacaac gcttggcaaa
      481 acgttaggcg aagagcatcg cgccgcagaa caagccaatc tcgccagccg cttcgttggt
      541 gaagctcagg cattcgccac ctcacccgcc gctaacctcc gcttttatgc tgcgcgcggt
      601 cctcgagggc tggaaacggg cttaaagggt tcgttgcata ccgaggcggc ggaattatta
      661 ggtttgcaca acgtcgcgca aatagccgat cgccacggcc tgacacaggt ttccatggaa
      721 aatctcctgc gctggcagcc ggatattatt ctggttcagg aggccgttac tgcagacttt
      781 attcgtcgtg atcctctctg gcagggcgtg aaagccgttg cggaacaacg catcttattt
      841 ttaagtggct tgccctttgg ctggctggat gccccaccgg gaatcaaccg tcttctggga
      901 ttacgcagac ttcacgcctg gctggatccc gccatcaatc gccagtttaa aagtgacatg
      961 cagcattacg cccaactgtt ctggcattgt tcactcagtg acgccgacta tcaaaaattg
     1021 gtggcgagct aatgagaatc gtaaacggat gtattttgct gctggcagct atcagtatca
     1081 cttttgccgc cgtaagtggg gcttatcatc tcgatatgca gcaactgctg gcgctaattc
     1141 ttcgtcaaga aaatgttcct gtgcaggaac aaattgtctt ctggcaaatc cgcgtgccgc
     1201 gtattcttgc tgcgctgtta ctgggcgcgg cgctggcagg agccggaacc acctatcagg
     1261 gaatgttgcg taatccctta gtttcaccgg atattttagg cgtctccgct ggtgccggac
     1321 ttggtgcctg cacggcaatt ctgtggggat tatccattgt ttacatccag ctgtacgcat
     1381 tttgcggcgg gctgatggtg gtcgcgggcg tctggttgat tacgcgtcgg gtgacccgcc
     1441 acgatccgat cttaaccctg gtgctggtag gcattgcgtt aggaacgttg tgcggagcgg
     1501 ggatatcact aattaaaacg ctggccgatc cttacaccca gttaccgtca attaccttct
     1561 ggttgctggg gggactgtcc accgtgacct tacacgatct ctgctatgct gcaccgatca
     1621 ttctcatagg ctccttgccc ctgttttttc tccgctggcg catgaattta ctcacgcttt
     1681 ccgacgacga agcccgttcg ctgggactga acgtgacgcg tctgcgcttt ggattgattg
     1741 tctgcgccac actaatcacc gccagtacgg tagcaatagc gggcataatt ggttgggtcg
     1801 gcctggtcgt tccgcacatt gcccgcttgt tgaccggtca taaccatcag cagctgttgc
     1861 ccatggcaat gtgtactggc gcgattctgc tcctgcttac cgatacgctg gcacgcagca
     1921 ttggaacaac agaaatccca cttggcattc tgaccgcttt tgttggcgca ccgtttttcc
     1981 tgcttttgtt gttacgcgga ggtcgccagt gactttgctt gccgtccgtc atgcgtctct
     2041 gggttactcc cgccatccgg tgctacgcga cgtgtccttc accctgtcgc aaggaacaat
     2101 ttgttgtctg ttaggagcca atggttgcgg caaaaccaca ctcatgcgct ccattcttgg
     2161 cgttataccg ttattaaaag gcgaggtctt actggatagc ctccctgtgc agacactcag
     2221 ccatcgccag cgcgcgcagg ctattgcctg ggtgccgcag gcgcacgacg gtatttttgc
     2281 ttttagtgtg ctggatatgg tgttgatggg cctggccccc accataggcg ctttttccgt
     2341 tcccggtaag caagaacgcc tgaaagccac ggagcaactg gaaaagctgg gcattttgca
     2401 tctggcggca cgacgctgga atacgcttag cggcggtgaa cgtcagctgg ctttaatcgc
     2461 ccgtgcactc gtccagcaac cgcgcttgtt gttactggat gagcctgcct ccagcctcga
     2521 tttcggtcat cagatccaac tactggacac gctggcacag ctcaaaaata acggcatgac
     2581 aatgttaatg tcgactcatc atccgctcca tgccaacgcc attgccgaca gcattatcca
     2641 ggtagagcca gatggacgag taacacaagg cttaccgacc gaacagttaa caacaaacaa
     2701 actggctgcc ctgtatcggg tttctgccga ccagattcat catcacctct ctgcaattag
     2761 ccactaagga aaaatcatgc tcattgatga tattgatttt gcagaccttt atctgcagca
     2821 actcaagctg gcccaccgca cagaaaaaac gccggatcac tgggatcaac gggcggaaaa
     2881 aatggcggaa aattgcgcct cgcctacgga cagctacctg caacaactga tcgctaaaat
     2941 tgacctacaa ggcgctcaga ccttattcga tatgggctgc ggaccgggta ccgtatcgct
     3001 ggcgctggct gacaaactca cgacgattta tggcgttgat tacagtcagg ggatgctgaa
     3061 cgttgccgcc cgtcgcgccg ccgcgctgaa ggcagacaat gtccactgga tccagcgcgc
     3121 ctgggaagag gactggagtg acctgccacg ctgcgatatt gccgtggctt cccgctccac
     3181 tctggtggct gatatgcacc aggcaatgag caaactgaat aatcaggcac ggcttcgcgt
     3241 ctatacgact catctggtca gcacctcgtt cgtctctcca gccattcagc gggcggctgg
     3301 tcgggaagtg atcgaattac ccaactatat ctttgcgctc aacgtcctgt atcagatggg
     3361 aatttatgcc cacgttgatt ttatccgtgg acaaaattgc caacaggata acagcacctg
     3421 ggaacgtttt gagcagaacg tcagctggag tcttggcgcg cttaacgacg atgagcgtga
     3481 gcggttatat cgctggtatc aacaacaaga tgctcgtgcc ctcgcccctg ccagccgcga
     3541 ctgggcgctc atctggtggg atagcgtacc gcaggaggca ctgcgatgat tttcctttct
     3601 caggcacaaa tcgatgcgtt acttctggaa gatatccagg gcggtgacct gaccacgaga
     3661 gcattaaata ttggacacca gcatggctat atagagtttt ttctccgtca gggcggctgc
     3721 gtcagcggcg tttctgtcgc gtgtaagatg ttaactactc tgggattaac tattgatgac
     3781 gcggtcagcg acggttcaca agcgaacgcg ggtcagcggc taatccgtgc gcagggtaat
     3841 gccgcagcac ttcatcaggg atggaaggca atccagaatg tgctggagtg gagttgcggt
     3901 gtttctgatt atctcgatca aatgctggcg ttacttcgtg aacgttatcc tgatggcaat
     3961 atcgcctgca cccgaaaagc aattccgggc acccgcttac tggcctcgca ggcaattctg
     4021 gctgccggag gactgattca tcgtgccgga tgtgcggaaa ccatattatt gtttgccaac
     4081 caccgccatt ttcttcatga caatcaggac tggtcaggcg caatcgatca gttacgtcgc
     4141 cacgcacccg agaagaaaat tgttgttgaa gccgacacgc cgaaagaggc aatcgccgcg
     4201 ttacgcgcgc aaccagacgt acttcagctc gacaaattta gtccgcaaca ggcaactgaa
     4261 attgcgcaaa tagcgccgtc gcgggctccc cactgcacgc tggctctgac cggcggaatt
     4321 aatctgacga cactcaaaaa ttacctcgac tgcggcattc gcctctttat tacctccgcg
     4381 ccatattacg cggcaccggc tgacattaaa gtcagtctgc aacccgcagc ctctatttaa
     4441 ttctttgtta aacggaattt attctatgag actcaaaaaa cattatctct gcacagcatt
     4501 atcacttgcc tttacccagc aggccgtagc ggctcaggag agcgactcgc tgaccgtatg
     4561 gtccagtccg gtatcatcga cgacgaccac cgttctcgat caacccacca tgaaggccct
     4621 ggataaacag aatgtcgctc aggcattaag tgtcgtcccc ggcgtggtgc tgcaaaagtc
     4681 aggcagccgc aacgaagaac aggtaaaagt tcgtggcttt gatagtcggc aggtgccggt
     4741 ctatttcgac ggtgtgccca tttatgttcc ctatgacggc aacctcgatc tggcgcggat
     4801 tctgaccaac aatctggggg cagtcgaagt ttccaaaggg tattcgtcgc tgcttcaggg
     4861 gcctaatcag atgggcggag ccattaatat caccacccag aagccaacaa aacctctgga
     4921 agcaagtctg ggatatcgcc agggatggag ccgtagccag gacaatgcct acgatatgca
     4981 tgcttcattt gccgccagta gcgatctggg gtatttgcaa gtcagcggta gccagctaaa
     5041 gcaggatttt ctcggcctgc cgcatggtgt aaataatgat attgcaggca aacacggcaa
     5101 gatgattaat tcatcggctg atgataaacg cggcattgtg aagctcggat ttacaccacg
     5161 tgaaaacgat gaatacacac tgacttacat taagcaggat ggtgaaaaag ataacccgcc
     5221 atacagcgga aatagtggtc aaaaatcacg ctactggcag tggccagagt atgacaaaga
     5281 aagtttttat tatcagggaa cgacccaact aaacgatcgt tttaccctga aaagtcggct
     5341 gtatcgcgac acctttgaaa atacgctgat gatgtacaac tcgctggctg atttgaaaaa
     5401 taaaaaaggc agctacagcc attattccga ttacagcgac ggtgccggat tacaactggc
     5461 agccgatgtg cgtgaaaacg atctgctgtc gtttgccgtt aactggaaag atgacgtaca
     5521 ccgggaaaaa ggtgcgccgc acgccgctta cgatcgctat gaagatcgta cctggtcgct
     5581 cgccagtgaa tatcaatggg ctgctgccga taatgtcgat gttgtggctg gaatcagcta
     5641 tgactggcgc gatagcgtag aagcgaaaaa acacgagaaa gatggcagca tcacccatta
     5701 tgacgacaac aatcagtcag cttttaactg gcaggtgatg gggaaatacc actttgtcaa
     5761 tgaagacacg ctggcgcttt cgtactatga ccgcacacgc tttccgacgc tgaaagaacg
     5821 ctataccaca tccaaacctg cgtataacca gatagcgatt gttaacccgc agctcaagcc
     5881 ggaacgcgct cgaggggtgg atttaacctg gaatggtgcc ttcacgcgcg actggggatt
     5941 tgaggtcagc gtttactata accgggtgag tgatgccatc ctctcgcaca atatcgatgc
     6001 cgataccatt caaaatcaga acagcggcac ggtggattac agcggtctgg atgccggtat
     6061 taaggggaaa atcagcaata tactggatgt aggattgagc tacgccctga tccacgctga
     6121 cgccaaacgt aaagacatcg gcaagataac cgatctgcca acacagacaa tgaccgcatg
     6181 gatgactctc aaaccgtggg agccgttaag cgtaacgctg tcggaagagg cgcgttcctc
     6241 cagctacagc aacagtgacg gttcacaaaa agccgccggt tttgcggtga cccacattcg
     6301 agccgattac accttaggtc atggcttcag cgtcaatgcg tcggtcaata acctgtttga
     6361 tacccaatat gcctacagtg aagggtttat tgaagaaggc cgcaatttct gggcaggtgt
     6421 cgagtatacg ttctgatgtg aaagcggata gcggcatctg tatcgctatc cgccttatga
     6481 aaatcttatt ctcttcgcag gaggcggaat acgcccagaa cgagaatagc tcccaccacc
     6541 gccaccagga agctgtgcaa attaaaaccg ctgatggagc cgccaatgcc aaacatggtc
     6601 gccagccagc cgccgaccac cgcaccgact atcccgagaa tacaggtcag gaaaaatcca
     6661 ccaccatcac gccccggcat gattagcttg gcgataatgc cggctatcag gccaaaaata
     6721 atccaggcaa taattcccat gttcaggccc tcgtttcgat gaatcggacg caagaaatgc
     6781 ttacgtcaac gttctaagta taggcaaggg cgggatcgat gctgggcgta tgtcggctga
     6841 tttaagaaca acatgtaaaa aatattcacg catttgtatt aagttttgtt aactgtgacc
     6901 gataaaccaa agacagtttg tcagtcagga gtttttccgc gtgagtcatt accatgagca
     6961 gttcctgaaa caaaatccgt tagccgtcct gggcgtgtta cgcgatttgc acaaagccgc
     7021 aattcctttg cgtatcagct ggaatggcgg gcagctgatc agcaaaatac tggtaataac
     7081 cccggataaa ctggtgctcg atttcggcag tcaagccgaa gacaacatcg ccgtactaaa
     7141 ggcacagcac attaccatta ccgccgaaac tcagggtgcg aaagtcgagt ttactgttga
     7201 acaactacaa cagagtgaat acttgcagct tccggcattt attaccgtac cgcctcctac
     7261 cttatggttt gtacaacgac gccgatattt ccgcatctcc gccccactcc atccgcctta
     7321 tttttgccag accaaactgg cggataacag tacgttacgt ttccgcctgt atgatttgtc
     7381 gttaggcggc atgggcgcat tactggaaac agcaaagcct gccgggttgc acgaaggtat
     7441 gcgcttcgct cagattgaag tcaacatggg gcaatggggt gtttttcact ttgacgccca
     7501 gttaatctcc atcagcgagc gcaaagtgat tgatggcaag aatgaaacca tcaccactcc
     7561 ccgtctgagc ttccgttttc ttaacgtcag cccgacggtg gagcggcaat tacagcggat
     7621 tattttctct ctcgagcggg aagcccggga gaaagcggac aaagtacgcg actgatttac
     7681 atcgcgtcca gtgcctgctc aagtttgtag atatagcgcg gagcctgcgg cgcaggatga
     7741 tttcgcgcta cgtgatcgag gaactcgtca gcatccagat cgttgatttt gctgattgcc
     7801 tttttccgat ctgacgagaa agtccgtagc agcgcacctg ccccgttagc atatgacacc
     7861 accagcgcat attgcagtac cttcggatct tcaattcctg ccagcgggcc ggtttccaga
     7921 atattcaggt aagccgcccc cattgaaata ttacgctccg gatttttcag ctcgctggtc
     7981 gtcggctcac cactccagcc catacggcga taaacatcac gaccggaggt tgaagctttt
     8041 aactgcatca aaccaatggc attcgattta ctcaccgcgt tcggattacc acccgattcg
     8101 atagcgataa tcgccgtgat caattgtgga tcgacgcccc aggctgcacc ggctttctgg
     8161 cttattggca tccactgcat cgcacgttgc accggaactt tcgcgttcca cggcgggttc
     8221 gtatagtcat gctttgatga acaacccgct aataacacaa tcaaaaaggc aaaccatctc
     8281 aatttcactt cttctatcct tatagcctgg aatcgccgtt gaggtgacaa cggcacacct
     8341 aagcggcatg atatacattt cgtttctgtt gtcagcaagg aattgccatg tctctgtttc
     8401 acttaattgc cccatcgggt tactgcatta aacagcacgc cgcgctgcgt ggtatccagc
     8461 gccttaccga cgcggggcat caagtcaata acgtcgaggt tattgcccgt cgttgtgagc
     8521 gttttgcggg tacggaaacg gagcgtctgg aagatctcaa ttcccttgcc agactgacta
     8581 cccccaacac catcgtgctg gctgtacgcg gcggttacgg tgccagtcgt ttactggcag
     8641 atattgactg gcaggcactg gtggctcgcc aacaacatga cccgttgctc atatgcgggc
     8701 atagcgattt taccgccatt cagtgcggtc ttctggcgca gggcaatgtc atcaccttta
     8761 gcggccccat gctggtagca aactttggcg cggatgaact gaacgccttt accgagcacc
     8821 atttctggct ggcgttacgc aataaaacct tcaccattga atggcaaggg gaaggcccga
     8881 catgtcagac cgaaggcacg ttgtggggag gcaatcttgc gatgctgatt tcactgattg
     8941 gtacaccgtg gatgccaaaa attgagaacg gtattctggt gctggaagat attaacgagc
     9001 accctttccg ggtcgaacgt atgctgttgc agctctatca tgcgggaatt ttgccacgtc
     9061 agaaggcgat cattctcggt agctttagcg gcagcacgcc caatgattat gacgcaggtt
     9121 acaacctgga gtcagtgtac gcgtttttgc gttcccgcct gtcgattcca ctcattaccg
     9181 gcctcgattt tggtcatgaa caacgcacgg ttacgctgcc gttgggcgca catgccattc
     9241 tgaataacac ccgggaaggc actcagttga cgattagcgg tcatcctgtt cttaaaatgt
     9301 aaaaaaatca cgcttcaggg ctaagtaaaa cgctgtctct gccgctaata tggtagggtt
     9361 tatataagag acagcgtaat caggagtaac cgaccgttgg atgccacaac aataattagc
     9421 cttttcatct taggatccat ccttgtcacc agcagtatat tacttagttc attttcttcc
     9481 cgtcttggca ttcctattct ggttattttt ctggcgatcg gcatgctggc aggagtcgat
     9541 ggcgtcggcg gtatcccgtt tgataattac cccttcgcct acatggtaag taacctggca
     9601 ctggcgatta ttttgctcga cggcgggatg cgcactcagg ccagttcctt tcgtgtggcg
     9661 ttaggaccgg cactgtcgct ggcgacgctg ggcgtgctta tcacctctgg tttaaccggc
     9721 atgatggcgg cgtggctgtt taatcttgat ttgattgaag gcttattaat cggcgctatc
     9781 gtcggctcta ccgatgctgc agcggtcttt tctctgctgg gtggtaaggg gcttaacgaa
     9841 cgtgttggct cgacgctgga aattgaatcc ggcagtaatg atccaatggc ggtctttctg
     9901 acgattaccc tgattgcgat gatccagcaa catgaaagca gtgtcagttg gatgttcgtg
     9961 gtcgatattc tgcagcaatt tggcctcggg attgtcatcg gacttggcgg tggttattta
    10021 ctgctgcaaa tgattaatcg catcgccctg cctgccggat tatatccatt gctggcatta
    10081 agcggcggta ttttaatttt tgctttaacc acggcgctgg aaggcagcgg tattctggct
    10141 gtttatctgt gcggttttct gctgggtaat cggccgattc gcaaccgcta cggcatcctg
    10201 caaaatttcg acggcctcgc ctggctggcg caaatcgcca tgttcctggt gctggggcta
    10261 ttggttaacc caagcgatct gctgcctatt gccattccgg cgctcatttt gtccgcatgg
    10321 atgattttct tcgcccgtcc tctttcggta tttgccggat tgctaccatt ccgtggcttc
    10381 aatctgcgtg agcgcgtgtt tatcagctgg gtgggattac gcggcgcggt gccaatcatc
    10441 ctggccgtgt tcccgatgat ggcggggctg gagaatgcgc gactgttctt taatgtcgcc
    10501 ttctttgtgg ttctggtttc actgctcttg cagggaacat cactctcgtg ggcggcgaaa
    10561 aaagccaaag tggtggttcc gcccgtcgga cgtccggtgt cacgcgttgg cctggatatt
    10621 catccggaaa atccgtggga gcagtttgtt tatcaattga gtgctgataa atggtgtgta
    10681 ggtgcggcac tgcgcgattt gcatatgcca aaagagacgc gtattgcagc cctgtttcgt
    10741 gataaccagt tgcttcatcc taccggcagc acccgactgc gcgaaggcga tgtgttgtgt
    10801 gtaattggtc gggaacgcga tcttccggcg ctcggtaaac tgttcagcca gtcgccgccg
    10861 gtcgcgctgg atcaacgctt ctttggtgac ttcattctcg aagccagcgc caaatatgct
    10921 gatgttgcgc tgatatatgg cctggaagac gggcgagaat atcgtgataa gcagcaaacg
    10981 ctgggtgaaa ttgtccagca gttgttaggc gcggcaccgg ttgtcggtga ccaggtagag
    11041 tttgccggga tgatctggac ggtggccgag aaagaagata atgaagtgtt gaagattggc
    11101 gtacgggtgg cagaagagga agctgaatct taaatttaat acagcatcta tcgccggttg
    11161 cgatgctttg cttaacgcag cctgagcctg tatataaact tgtcatttcc tgatttgata
    11221 tgcacattcc aacatcagaa gaaggttaaa ttatggatga caaaccattg caggctctgg
    11281 ccaatcaact caaaacaccg gctcaagaaa gtcagcgtaa tggcttcttc tgactgcaac
    11341 aggtgaagaa taaccagccc cgcattaccg gtatccccgt caaccatcag gggccccagc
    11401 ctgacatcgc ctgaacatat tcccccacgc aacctgccgc actggcgtag cccacacaat
    11461 acgcgtttca gcctgctcag tatctgtagt ggcgtttcat ccgcctgtag caccggatgc
    11521 atgagcgatt tcttatagtt attcctttgc ttaataacga ttatttccgg gtcgttcgct
    11581 ctcgtatttt ctgtattacg tcagagctgc ctgggccgta atacagcatg taccagaccc
    11641 gacacggcat ttctgaccaa gggaaaaagt caacgatgag tagttatatc agcgcaaatg
    11701 acgtcctgtg gcacctttgc ggtgaccaca ttcacgtctc cggcgctgcc agaggatatt
    11761 tatttcaagg atacgtagca ttatggccag cattaaagac atccgcccat tcgcccccgg
    11821 cattccccca tcttccgtta acccaggcgt acgacgccca ccagggcagt acttcctgcc
    11881 ggtggaactc aagaatgaaa ttttgcggaa catgaagtat atcggcccgc aaatatttag
    11941 ctggggtgat gaccaccgtc agatcattga cctcagtacc gcgggcaagg tgaaaataca
    12001 tatggtagac aataccatca tcatgttgga gcgcctgaat ccgaatccgc aacataacga
    12061 gttcatgatg gtcgccgaat ggtgccaatg ggctatggag cgggatggtt tgcaagaaaa
    12121 cctgcaaaaa aacctgtacg aaatcctgga agaaaaccag caaaacaaac agtcagaaat
    12181 tccccaagaa gacctgaaag aaagtctgga agaaatcaag gtaaatatcc tggaagaaaa
    12241 cctgccagca agccggattg aaaaccgggc agaagcattg cgcagaatga aggaatgtct
    12301 cattacaaga cggagtatgc tcaatcttag caaccttgga ttgacttcac tccctgaaaa
    12361 tttgcctcca catctgattg aattttattg cagtaaaaac gtgttgaccg cgttaccgaa
    12421 ggtgatgcca aagtggctgc tggtgcttga ctgtacagat aacgtcttga tcttgttacc
    12481 gaaggtgcag ccctcaaaac tgatggtact gaattgctat gacaactgta ttatctggct
    12541 gcctgagctg tcgactaacc tgagagtgat taactgttct gcaaacttct tgcaattttt
    12601 accgccgtcg atgccccagt acctgtataa actgtcctgt gctggcaaca atattcactc
    12661 aatacctgat gagatgctgg agaacttgac tcgcctgaaa gtatttgact gttctagtaa
    12721 cgatttgatc tcttcaccac ggctgccgcc caaactaata atttactact gtggagaaaa
    12781 caagtttaaa acggtacagg tgccgcagcc ccagagcctg aaggtgtttg actgtaatgg
    12841 taacccgtgg gacaaagaca atttaccgac gctgctcaaa gccgtcgagg gcctgaaaaa
    12901 ccaggagggc ctggtggacc ttttagactt tttgcgcaag gaaggccagt ttgacctgga
    12961 gggactcgag gagctggagg acctggagga cctgatggtt ctggagcacc tggaggaccc
    13021 ggtactcctg gagcgcgtga aggtacag
//
LOCUS       DAPGOF010000135        13020 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.136, whole genome shotgun sequence.
ACCESSION   DAPGOF010000135 DAPGOF010000000
VERSION     DAPGOF010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13020
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.136"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            398..1087
                     /gene="mutH"
                     /locus_tag="RHV13_003468"
     CDS             398..1087
                     /gene="mutH"
                     /locus_tag="RHV13_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HDV1242970.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            1156..1869
                     /locus_tag="RHV13_003469"
     CDS             1156..1869
                     /locus_tag="RHV13_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDV1242971.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            2007..2225
                     /gene="ygdR"
                     /locus_tag="RHV13_003470"
     CDS             2007..2225
                     /gene="ygdR"
                     /locus_tag="RHV13_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HDV1242972.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            2333..3373
                     /locus_tag="RHV13_003471"
     CDS             2333..3373
                     /locus_tag="RHV13_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HDV1242973.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMEQLKTNVESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(3405..4598)
                     /gene="lplT"
                     /locus_tag="RHV13_003472"
     CDS             complement(3405..4598)
                     /gene="lplT"
                     /locus_tag="RHV13_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HDV1242974.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWCNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(4591..6750)
                     /gene="aas"
                     /locus_tag="RHV13_003473"
     CDS             complement(4591..6750)
                     /gene="aas"
                     /locus_tag="RHV13_003473"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HDV1242975.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LALFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKRLVKRRLFPQI
                     TLHILPPTKVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            7336..8367
                     /gene="galR"
                     /locus_tag="RHV13_003474"
     CDS             7336..8367
                     /gene="galR"
                     /locus_tag="RHV13_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HDV1242976.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(8374..9636)
                     /gene="lysA"
                     /locus_tag="RHV13_003475"
     CDS             complement(8374..9636)
                     /gene="lysA"
                     /locus_tag="RHV13_003475"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDV1242977.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDNIVFTAD
                     VIEQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVLEFGQD
                     LQAISAGGGLSVPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            9758..10693
                     /gene="lysR"
                     /locus_tag="RHV13_003476"
     CDS             9758..10693
                     /gene="lysR"
                     /locus_tag="RHV13_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HDV1242978.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(10680..11372)
                     /gene="ygeA"
                     /locus_tag="RHV13_003477"
     CDS             complement(10680..11372)
                     /gene="ygeA"
                     /locus_tag="RHV13_003477"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HDV1242979.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCSLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(11501..12919)
                     /gene="araE"
                     /locus_tag="RHV13_003478"
     CDS             complement(11501..12919)
                     /gene="araE"
                     /locus_tag="RHV13_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HDV1242980.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT         3302 a         3499 c         3208 g         3011 t
ORIGIN      
        1 ttaagtaatg aaaaagagta attcgtgacc caggtcacac ctctcattta cgggttggcg
       61 aaagatcaac caatgccgta tttatccaca gaatgtgcca ctaagttaag cactgaacca
      121 ctaaaaactg gagtttcgtc gcacgtcaag gctgtaaatg gaaacagtag tggaggtttt
      181 tcacagttat cccagctttc tgtggataac atggtgtaag atcctgttta ttttcagtga
      241 ccagatttgg aaaacccgtt gcagtgttgc gcaactcgat taccggtaac ctaaaaagca
      301 atataaatca gagaattgaa caacgcatgt ggaaaaagtt acactgcgaa tattcggcac
      361 ataattgctg tttgtttttt aaccaaggta tcatgacatg tcccaacctc gcccactgct
      421 ctctcctccc gaaactgaag aacagttgtt agcgcaagca cagcaacttt ctggttatac
      481 attgggagaa ctggcggcac ttgccgggct ggttacgcca gagaatttaa aacgcgataa
      541 gggctggatt ggcgtgttac tggagatctg gctaggtgcc agcgcaggga gtaaacctga
      601 gcaagatttt gccgctctgg gcgtggaact taaaactatc cctgtggata gtcttggtcg
      661 tccgctggaa acaacattcg tttgtgttgc cccgttaacg ggtaatagcg gggtgacctg
      721 ggaaaccagc cacgtgcgcc acaagctcaa acgcgtactg tggataccgg ttgaaggcga
      781 gcgcagcatc ccgctggcgc agcgtcgcgt aggatccccg ttgctgtgga gcccgaatga
      841 agaggaagac cgacagctac gcgaagactg ggaagaatta atggatatga ttgttctcgg
      901 tcaggttgag cggatcaccg ctcgtcacgg ggaatattta cagataagac cgaaagcagc
      961 gaatgcgaaa gcgcttaccg aagccattgg tgcccggggc gaacggattc tgacgctgcc
     1021 acgcggcttt tatttgaaga agaatttcac cagtgcacta ctggcccgtc attttctgat
     1081 ccagtagcca tcgctttgac ctgccgcttt ccgggcatat aattaccgct tcattttttt
     1141 ggcagggctt tttagatgtt atttgcatgg ataaccgatc ctaacgcctg gcttgcgctc
     1201 ggtacgctga cgctgctgga gatcgttctt gggatcgaca atattatttt cctttctctg
     1261 gtggtggcaa agcttcccac agcacaacgt gctcatgcgc gccgtctggg gttggcggga
     1321 gccatggtta tgcgtctggc gctgctggca tcaatcgcct gggttacgcg cctgacaaat
     1381 ccgcttttta caatattcag tcaggaaatt tctgcccgtg atttgattct gcttctgggt
     1441 ggcttgttcc ttatctggaa agccagcaag gaaatccacg aatccattga aggtgaagaa
     1501 gaagggctga aaacacgcgt ttcatcattc ctcggcgcta tcgtgcagat tatgctgctg
     1561 gatattatct ttagcctcga ctcggtgatt acggctgtgg gtctgtcaga tcacctgttt
     1621 attatgatgg cagccgtggt gattgccgta ggcgtgatga tgttcgccgc gcgctcgatt
     1681 ggtgattttg tcgaacgcca tccttcggta aaaatgctgg cgctctcttt cctgattctg
     1741 gtgggcttta ccctgattct ggaaagtttc gacatccacg taccgaaagg ttacatctac
     1801 ttcgcgatgt tcttctctat tgcggttgaa agcctcaact tgattcgcaa caaaaagaat
     1861 ccgctctgat actccgtacg ctctcctgcg ggagggcgta ctcactcctt ttaccctcac
     1921 agtttaagat tttcctgctt tcaagatata taacgtcggt ttataaacag actattatca
     1981 taggtaggcc aaatgaggat aaccggatga aaaaatgggc agtaataatt tctgcagtcg
     2041 gactggcgtt tgctgtttcc gggtgttcca gtgattacgt catggcgacc aaagatggcc
     2101 gtatgatttt gaccgatgga aaacctgaaa ttgatgatga taccgggctg gtgagttatc
     2161 acgatcagca aggtaacgcg atgcaaatta accgtgatga tgtttcgcaa attattgaac
     2221 gttaacaaat aaggtcagca tccggctggc cttaagattt ttctctttcc cttttccctt
     2281 ccctctgcca tttttatatt ccttatgtcg tgattataaa aaggaaacgg ctatgcaata
     2341 tcaccgtata ccccacagtt cgctggaagt cagcacgctg gggcttggca cgatgacgtt
     2401 tggtgaacag aacagcgaag ccgacgccca cgcacaactc gactatgccg tcgctcaggg
     2461 cattaacctt atcgacgttg ccgaaatgta cccagtacct ccgcgccccg aaacgcaagg
     2521 gttaaccgaa acctacgtcg gcaactggct ggcgaaacat ggcagccgcg aaaagttaat
     2581 tatcgcctcc aaagtgagcg gaccgtcgcg caataatgac aagggcatcc gcccggatca
     2641 ggcgctggat cggaagaata tccgcgaagc gctgcatgac agcctcaagc gcctacagac
     2701 tgattacctc gatctttatc aggtgcactg gccgcagcgc ccgaccaact gcttcggcaa
     2761 actcggttat agctggacgg attctgcgcc tgcggtttcg ctgctggata cgctggacgc
     2821 actggcagag taccaacgcg cgggaaaaat tcgttatatc ggcgtgtcga acgaaactgc
     2881 atttggcgta atgcgctacc tgcatctggc ggacaaacac gatctgccgc gtattgtcac
     2941 cattcagaac ccttacagtc tgttaaaccg cagttttgaa gtaggtctgg ctgaagtcag
     3001 ccagtatgaa ggggtcgaac tgctggccta ttcgtgcctg ggtttcggca cgctgaccgg
     3061 gaaatatctc aatggtgcaa aacccgctgg cgcacgtaat acgctcttta gtcggttcac
     3121 ccgctatagc ggtgagcaaa cgcaaaaagc cgtcgcggcg tatgttgata tcgccagacg
     3181 tcatggcctg gaccctgctc agatggcgct cgcttttgta cgtcgtcaac cgtttgttgc
     3241 cagcactctg ctgggcgcaa ccacgatgga gcagctgaaa actaacgtcg aaagtttgca
     3301 tctggagttg agcgaagacg tattagctga aattgaagcg gtgcatcagg tttatacgta
     3361 tcctgcacca taagattaac gccggttaaa accggcgtta aatattaatg acggcgctgc
     3421 cagatccaca gcgccgttat tgccagcgca aatagcgcac cgaagccaat gccaatgggc
     3481 acgaccggga tgcctaccat taccgccagc gagtaaatgc ccagcatcaa caacatggcg
     3541 ctgttttcgc caaggttttg tactgcaatc gcattccccg ccccgacgct ttttttaccc
     3601 cgctcctgta gcaacgcatt gagcggaacg acaaaaaagc cccccagcac accaatcagc
     3661 atcaacaagg cataggctgg cagcagctcg tgttgcaggg aaaaaatcag taccaccacg
     3721 ccaatcaaaa tcccggctgg catacagcgt gacacggttt ccagcgtaac cagcttcgct
     3781 gccgcacctg cgccaaccac gataccaatc gctaccatcg cgttgagata ggtcggcgta
     3841 gcgttatcgg taatgcccag cgccaccggt acccacagca ccaacaggaa acgcagcgtg
     3901 acacccgctc cccagaataa actggtgccc accagcgaaa aacgcgtttc accattgcac
     3961 catagcgagg tgcaggcatt caggaaactg cgggtcatgt tgatgagatt ccaggactgc
     4021 cccggacgcg ccgccgccag tttgggaatg tagatattgg caacgaccgc accaccgtag
     4081 gccagtgcgc atgcggccag ggcgaccagg acatgccagt cagccagcac accaccggct
     4141 acggaaccga gcaaaatcgc cgctatggta gaagcttcca ttaaaccgtt agcttttact
     4201 aacttactac ccgtggttaa ttcgccgaga ataccgtatt tcgccggtga ataggctgca
     4261 gcaccaacac ccaccagcgt atagccgaga aacggattga taccaaagca gatactggct
     4321 gcgcccagca gcttcaggcc gttggcaaac atcatcaccc ggcctttggc gaagctatcc
     4381 gccacctgcc cgacaaacgg cgcaaaaaga atgtaagcac ctacaaacac catttgcagg
     4441 atgggctggc tccactccgg atagaactgc gctttcagta acgccagagt ggcaaacagt
     4501 agtgcattat cgccaaacgc agagagaaac tgcgccacga taaccgcttt catccccttc
     4561 gaccacaacg aagtgttagt gtgcactgac tcactcatcg tgttgttccg cttcgtctac
     4621 ccagcttttc aacgtgacaa agtcaggttt accgctgcca agtaatggca tctgtttcag
     4681 atagcgaata tcgcgcggta cagcaagctc cggcacgccg tgctcgcggg catactgttg
     4741 caacttatcg cgcgtcagtt cgttatctgt ggtgaaaagc accagtgcct cgcctttact
     4801 ggcatcgctc ttaatcgcag tggcatggac tttatctggc gaaacaccaa gtgccagttg
     4861 ttccaccatt tccagcgata ccatttcgcc tgcaattttg gcaaagcgtt ttgcgcggcc
     4921 ctgaatctgc acaaagccct gctcgtcaaa acgcacgata tcgccagtgt cataccagcc
     4981 acgttccatt tcgccgcgaa cattctcggc ggtgggcact tccagtacac ctggcttctc
     5041 cacccgcaga tagccgttca ttatgttcgg ccctttcagt tgcaggcgtc cgccctcttc
     5101 gataccagga accgacaaca ggcgcgcatc cattcctggt agaatacgcc ctaccgtacc
     5161 gggtttcgcc gccatcggta cgttgataga aacgacaggc gcgcactcgg ttacaccata
     5221 gccttcaagg atgcgcaggc caaatttatc ctgccaaagc tgtttggtac tttcttgtag
     5281 tttttctgcg cctgccacca catagcgtag acgatagaag tcatacgggt tggcgaaacg
     5341 cgcgtagtga ccgaggaaag tggaggtacc gaacaacacg gtgcaactgc ggtcatacac
     5401 caactccggc acaatgcggt aatgcagcgg gcttggataa aggaacactt ctgcacctgt
     5461 gagcagtggc gtaaacaggc ctaccgtcag cccaaaggag tgaaacagcg gcaacgccga
     5521 cataaagcga tcgttggtgg tgaagtcggc aatcgtttta atctgctcga cattcgccag
     5581 aatgctttta tggctatgga cgacgccttt cggatggcct tcagaaccgg aggtaaaaag
     5641 gatcagcgcc tcttcttccg gctgctgttt tacctgtgcc agacgcggca tcagcaaatg
     5701 agcgaagatc cacactttgt cggcagtggt gacatctgct tttaaatctt ccagatagac
     5761 ccagcgcacc tgagtaagtt gctccggcag atgccagagt ttgcctttat cgagaaactg
     5821 gcgggaagtg aagatggttt tgatttcagc cgccgtaatg gcgctggtca gcccttttac
     5881 cccagcggtg tagttcatca ttgcgggaat gcggcgacgg gcgatggccc caaaaatcac
     5941 tgccgcactg atgcctgcat tgggcagcat taagccgatg cgttcgcctt caacactgta
     6001 tttttcaagg atgcgtccaa caaacagcgt tttagtaagc aatttgcgat aggagtctgg
     6061 ggtaaagttg acgtcttcga cacatttctt cccggctccg aagcggtaca ttgcactcag
     6121 taaagattcg tacagcgttt cacgcgggcg caccgccata cgcgcttcca tcattatttg
     6181 atgcagcatt tcgccagcga ttttgcgacg gtcacgggca cgcggcgcat ccggcatctc
     6241 caccttcgtt ggtggcaaaa tatgcagagt aatttgcggg aacaagcgac gtttaaccag
     6301 acgtttcagg cggctgaagt gcgtaagttc tgccccttcg atacgcacag gaataaccgt
     6361 tgcaccagac ttcgccgcga caaaacccgc gccatcgtag attttcatca gcgagccagt
     6421 ggtggtgatg cgtccttcag ggaaaatcac cactggtcgg ccctgttcca ccagacgtac
     6481 cagatgttta atcgccatag gttgcgtcgg gtcgagagga acaaagtcga taaatgattt
     6541 cagccaacgc atataccact gttggcttat tgaggtgtaa acggcaaata ccggacgtac
     6601 gggtaaaaac aatgccagca gtatgccatc aataaaagag acgtgattag gcgtgattag
     6661 aacgcgctcg cccttcagtg cctgggtgtc acccgtaacg cgaacgcgat acaaaacacg
     6721 gcacaaattt cgaaaaaagc taaaaagcat tccaactccc tttgctctga ttcagtaaaa
     6781 gcgaatggag ggagattaca cgagataaag aacgcgagcg acagtaaatt aggtgcgaaa
     6841 aaaaacctgc gcatccgcgc aggttggtgc aagagacagg gtacgaagag cgtaccgaat
     6901 aatctcacca atcaatacct ctgggatctt gattgtggtc tgcacgacga ctcttcgcca
     6961 gcgagaaaac gcaaaggaat gaagggaaat gcaacgaggt gtgtaaattg tcggttactg
     7021 ttacagtttg atgaccggca aaaaaaaacc tgcgcatctg cgcaggctgg tgtaattcat
     7081 gtgctcaacc cgaagttgac ttcacctatc aatacctctg ggatcaccac tttagcaacc
     7141 tgaagccaaa cgccaccagc ggtcaatcgc aacagcgttt cgcaaagtgt aaccaaaggt
     7201 ttgcaatctc tttttttgtc ttacttattt gcgtttggct cacattccca cgatgaaaac
     7261 acgccgcccc ttgaaccaac gggcgttttc cgtaacactg aaagaatgta agcgtttacc
     7321 cactaaggta ttttcatggc gaccataaag gatgtagccc gactggcagg cgtttcagtc
     7381 gccaccgttt cccgcgtcat taataattca cccaaagcca gcgaagcttc ccggctggct
     7441 gtgcatagtg caatggagtc tcttagctat cacccgaacg ccaacgcccg tgcgctggca
     7501 cagcagacca ctgaaacggt cggtctggtc gttggtgatg tttccgatcc gtttttcggt
     7561 gcaatggtga aagcggtcga acaggtggct tatcacaccg gtaatttctt attgattggc
     7621 aacggttacc acaacgaaca aaaagagcgt caggctattg agcaactgat ccgccatcgc
     7681 tgtgctgcgt tggtcgtcca tgccaaaatg atcccggatg ctgatttagc ctcattaatg
     7741 aaacaaatgc ccggtatggt gctgatcaac cgtatcctgc ctggctttga aaaccgttgt
     7801 attgctctgg acgatcgtta cggtgcctgg ctggcaacgc gtcatctaat tcagcaaggt
     7861 catacccgca ttggttatct gtgctctaac cactctattt ctgacgccga agatcgtctg
     7921 caagggtatt acgatgccct tgctgaaagt ggtattccgg ccaatgatcg gctggtgaca
     7981 tttggcgaac cagacgaaag cggcggcgaa caggcaatga ccgagctttt gggacgagga
     8041 agaaatttca ctgcggtagc ctgttataac gattcaatgg cggcgggtgc gatgggcgtt
     8101 ctcaatgata atggtattga tgtaccgggc gaaatttcgt taattggctt tgatgatgtg
     8161 ctggtgtcac gctatgtgcg cccgcgcctg accaccgtgc gttacccaat cgtgacgatg
     8221 gcgacccagg ctgccgaact ggctttggcg ctggcggata atcgccctct cccggaaatc
     8281 actaatgtat ttagtccgac gctggtacgt cgccattcag tgtcaactcc gtcgctggag
     8341 gcaagtcatc atgcaaccag cgactaaccg cagttaaagc aattccagcg ccagtaattc
     8401 ttcgatggtc tggcgacggc gaatcaatcg cgcctgacca ttatcaaaca gaacttctgg
     8461 taacagcgga cggctgttgt agttggatga cattgatgcg ccatatgccc ctgtatcatg
     8521 cagtaccaga taatcacccg ccttcacttc cggcaaggcg cgggtttcga catttccccc
     8581 ttcctgctgg gtaaagacat cgcccgattc acataacggc ccggcgacga cggtttccac
     8641 cgttggcgcg tgttccagag aacgaccatc agctgccaga gcactgatat ggtggtaact
     8701 accgtacatt gccgggcgca tcagatcgtt gaacccggca tcaacaagca caaagtggcg
     8761 gctacccatt tgtttgacgc tccgcacctg agtaattaat acgccagact gcgctaccag
     8821 gaagcgaccc ggttcaattt ccagtttcac agggtggccc aaatggcggg caatttgctc
     8881 acgcgcggca ttccacagac cataataatg ttcggtatca accgcctctt caccctgttg
     8941 ataaggaaca gaaagcccac cgcccgcaga aatagcctgt aaatcctgac cgaattcgag
     9001 gacctgacgc accatcgcac cacacacctg ctccagatgg gcataatcaa cgccagaacc
     9061 aatgtgcatg tgaatgccga ccagctgcag atgatgacgt tgtatcacat ccagtgcggc
     9121 gggcagatcg gtgtaccaga taccgtgctt gctgttttcg ccaccggtat tggttttttg
     9181 gctatgtccg tgaccaaacc ccgggttaac gcgcagccat acccgatgcc ctggcgaaac
     9241 ctggcccagt tggtcgagca tatcaacaga acccgcattc accggaattt gcaattcact
     9301 gacgcgttca agcgtcgcct gttcgataac atctgcagta aaaacaatat tatcggggtg
     9361 cgtttgcgga ttgtaacccg ccgccaacgc acgctctatt tcgcctaacg agacggaatc
     9421 cactttcacg ccctgctcac gcattaagcg caaaatatga atattggaac aggctttctg
     9481 tgcaaagcgc accacatcaa actgtttcag cgctgcaatc tgccgacgaa taatttgcgc
     9541 atcgtagacc cacaccgggc agccaaattc agcgggcaaa cgcagcagat tttcggcggt
     9601 gagatcggta tcggtactga acagtgaatg tggcataaca aactccagat aagtgctttt
     9661 ttatgattac gccacatcat aaaaagaata aaaaatatcg atttatgtcg agtctatgca
     9721 aaaatgatat ggattaccgg attgcgagag agcgctaatg gccgccgtta acttacgtca
     9781 tattgaaatt tttcatgcgg taatgaccgc cggaagcctg actgaggcgg cacacctgct
     9841 acacacctca cagccaaccg tcagccgcga actggcgcgc tttgagaagg tgatcgggct
     9901 aaaattgttt gagcgcgtac gtggacgatt acatcctacc gtgcaaggac tgcgtctgtt
     9961 tgaagaagtg caacgatcct ggtacggact ggatcgcatt gtcagcgccg cagaaagtct
    10021 gcgcgagttt cgccagggag aactgtctat tgcctgcctg ccagtctttt cgcaatcttt
    10081 tttaccgcag ctcctgcaac cctttctggc acgttatccc gatgtcagct taaatatcgt
    10141 gccccaggaa tcaccgctac ttgaagagtg gctctcggcc cagcgtcatg atttagggtt
    10201 aactgagacg ctccatacgc ctgcgggaac agaacgtacc gaattactct ctttagatga
    10261 agtgtgtgtg ttacctccgg gtcatccgct ggcggtaaaa aaggtattaa cgccggatga
    10321 ttttcagggt gagaactaca tcagcctttc ccgtactgac agctatcgcc agttgctgga
    10381 tcagctattt actgaacatc aggttaaacg acgcatgatc gtagaaaccc acagcgccgc
    10441 ttcagtctgc gcaatggtac gggcgggggt aggtgtttcg gtggttaacc cgctcaccgc
    10501 actggattat gcggcaagcg gtttagtggt gcggcggttc agtattgcgg ttccgttcac
    10561 cgtcagcctg atccgccctc tgcatcgccc gtcatcagcg ctggtgcagg cgtttagtga
    10621 gcatttacaa gcggggttac cgaaactggt cacttctctt gacgctattc tgtcgtcagc
    10681 tacgacagca taaaagcgac agcatcctcg gcatggatcg ccgcggtatc aaacacaggc
    10741 aaaacactgc gctcttctgg aaccaataaa ccaatttctg tgcagccaaa aatgacgccc
    10801 tgtgcgccct gttctgccag gcgagcaatc acttgcgcat aataagcgcg tgacgcttcg
    10861 gtaaattgcc ccagacacag ttcttcaaaa ataatctgat taatcttcgc ccgttcatcc
    10921 gcttcaggaa taagacaatt gatggaaaat tgttccgtca gccgcccgcg ataaaaatcc
    10981 tgttccatgg tgtaacgcgt acccagcagc gccacacgag tcattccggc cccggtaatt
    11041 gcacgtccgg tggcatccgc aatgtgtaag aaaggcagag agcaacgaga ctctatagca
    11101 tccgccactt tgtgcatcgt attggtacat aacacaatgc cttctgcgcc cgcccgctgt
    11161 aagccaagcg ccgcctcagc cagaatatcc ccggttttgt cccactcgcc gcgacgctgg
    11221 cactcttcta tttcatgaaa atcgacgcta tgtagcagca cttgcgcaga gtgaagccca
    11281 ccaagccgct gtttaatgcc ttcatttatc agacgatagt aaggaatagt ggattcccag
    11341 ctcattcctc ccagcaaacc aattgttttc attcctctct cctgatgtgt gttaccgcaa
    11401 caaggctgaa taaggaacga gacaaacgcc tcaacggcca ggtgcccaat ctctattaac
    11461 gaaaaaaggg ccggatgtac agcacatccg gcccgtgaaa tcagacgccg atatttctca
    11521 acttctcgcc tgccatcagt ttgcgttcga tatgttccag ggtgacattt ttggtttccg
    11581 gaatgagcca gaaagtgatg cccacaaacg caatgttcag cgcagtgtag agccagaacg
    11641 taccggcagc gccaatgctg tcgagcagcg tcaggaaggt cgcgccgata atcatattcg
    11701 acacccagtt cgtggtggtc gaacaggtaa taccgaaatc gcggcatttc agcggctgaa
    11761 tttcagagca caggatccac accactggcg cggcgctcat cgcataaccg gcaatacaca
    11821 tcatcgtcat gccaacagag agccaggaca agccactgga agccgtaccg ttatcaaact
    11881 gcatcaggca atagcccagc accagagtgc ctaacgccat cacgctgaaa ccaattttca
    11941 gagccggttt acgacctgct ttatctaccg taaagaccgc aatgaaggtc gcgaacataa
    12001 aggtcagtcc cacgaccaga gtcgcaatca tctgttgttc tgtggtcgta aagcccgcca
    12061 ttttgaagat acgcggcgcg tagtacatga tgatgttcat accggtaaac tgctgcatcg
    12121 cctgcaacaa cataccgaga aacacagcac gacggacgtt acggttgatc ttaaacagtg
    12181 cccaaccgcc ctgttttaac ttcaggcttt cacgaatttc gttgagttct tctcgcgctt
    12241 tttccgacgt atcgcgcagc atacgcaaca cttcttccgc ctcaatatga cgcccctttt
    12301 ccgccagcca gcgcgggcta tttggcagga agaccaccag aataatcagc aggactgctg
    12361 gtaaagcaag aacccccaac attgcgcgcc agttaccgct ataactgaac gctgtatcgg
    12421 ataaaaacgc cagcacaatg ccgagtgtga ccatcaactg gtacatactg atcatcttac
    12481 cgcgaacgtt ttcacttgcc atttcagaaa gatacagagg agcggtgtaa gacgcgatcc
    12541 cgacagcaat gcccagcacc acacgagcgg cgattaacat ctctacgctg gtcgcaaaag
    12601 cggaccctat agaaccgagt acaaacagga tggcccccgc catcaggctg tatttacgcc
    12661 ccaggcggaa cgacagccaa ccattaaaca gcgcaccaat tgctgcgccg agcatcatgc
    12721 tactaaccac ccattcctgc aaacgactgg tcagcacaaa gtgatcggta atgaacggca
    12781 acgctccggc gattacgccg atatcaagac caaataacaa tcctgcgacc gcagcagcta
    12841 ccgaaacaaa catattcata cgccgcgtat cccgcaaaga acgtggcgtt aaagcagatt
    12901 ccgtattgat agtaaccatt ttttcctgcc agcagagagt aagacatagt gaaaaaatac
    12961 gtgaacaact cacacaggtg tcaggtcgga aacagcataa atatggatta aattgctact
//
LOCUS       DAPGOF010000136        12859 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.137, whole genome shotgun sequence.
ACCESSION   DAPGOF010000136 DAPGOF010000000
VERSION     DAPGOF010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.137"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(153..1610)
                     /locus_tag="RHV13_003479"
     CDS             complement(153..1610)
                     /locus_tag="RHV13_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HDV1242981.1"
                     /translation="MQEFPFNAFPSSLLETIFDIEECTRAPGEMIALTLLSGLSLACQ
                     SLINVKITDTMKSPVSLYSFVIADSGERKTAVEKLLLKPFHEHDLRVLLQYEKQKKEH
                     EVQSKIWSKKENALLKCIDKDTMKGLCTDKLSRQLEQLSAEKPVQPKAKRYIYNNVTP
                     EALQFEMYTHSPQTGLITDEGANILSRRTMSDLGFMNSIWDDESFHVNRKTGPSFTIK
                     NGRITLLVMVQKAIFYDFLKRKGEHARGSGLFARCFILYIDAKLSTQGQRFISRLSSP
                     QSGKLENLNRFYQRMNELIEKSASQHGTGHQTCLSFEPSALAAWKEIHDEIESSIGPD
                     REYANMNDFASKLPNNVARLAALLSYYTEGECAIKKEYVENAWLLCEWYMQQAIKVFG
                     AQEGYYEALLLSWLRREYYETEMDHVRFNTIRNAGPNVLRKGKLLERVIERLEKEGAI
                     DIVRSRRKARMVYAGRYFRNNPFTKNAAYKHYWPR"
     gene            1925..2812
                     /locus_tag="RHV13_003480"
     CDS             1925..2812
                     /locus_tag="RHV13_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004128339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HDV1242982.1"
                     /translation="MKHPSGYTLIRRHLRRYPHALRQHLLQELNRLVTYEPVIGIMGK
                     TGVGKSSLCNALFRSEVCTVNAVEACTRQPQRVRLRFGSHYLTLVDLPGVGESVTRDG
                     EYRELYRDLMPQLDMVLWVLKADDRAFAVEEQFYQDVFAQFRGPIPPVLWILNQVDKT
                     EPAEQWRWLSAQPSALQAERIAQKRQAVARQLQIAEIDILPVSVRGRYRLPRLVETMI
                     TRLPKQARSPLVPHLQNRYRTDTVINTASSSFGDAVVEVIDNIIDLAPLPQVARTALH
                     AVTHTVARAAGSLWGFFFG"
     gene            3025..3183
                     /locus_tag="RHV13_003481"
     CDS             3025..3183
                     /locus_tag="RHV13_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001128372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242983.1"
                     /translation="MPLILLWGGLALLLGIVASANGRSFWGWFILGLIIDPILAGLLY
                     WLIAKDRT"
     gene            3309..3773
                     /locus_tag="RHV13_003482"
     CDS             3309..3773
                     /locus_tag="RHV13_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242984.1"
                     /translation="MKNKLVISFLLASVLTPSVSHAFGNPDSWVSGYAQGTSEYTILG
                     KGQSQLYLACDSSGSQPATIIFTDVNGQQVRMDSGQTLAMRIDNAEEVNISESESHVG
                     SDNVMWAWNKLRTGKRVVVSGSGVKPATFTLAGAAAVLPAFGDNGCVPGFAL"
     gene            3808..4149
                     /locus_tag="RHV13_003483"
     CDS             3808..4149
                     /locus_tag="RHV13_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1242985.1"
                     /translation="MKKIITVAALSLLSFSSLAGSSPVSVSVSPGSYSHYSSIRVTSK
                     VDSIVIKQLIVNRGNCQDAEFASPWKPVRLGFGGAVSHEFTGKGLMVPCNVLEVVVQT
                     SEGAWQFDFDS"
     gene            4241..5062
                     /locus_tag="RHV13_003484"
     CDS             4241..5062
                     /locus_tag="RHV13_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HDV1242986.1"
                     /translation="MRLASRFGRINQIRRDRPLTHEELMSHVPSVFGSDKHESRSDRY
                     TYIPTITILESLQREGFQPFFACQTKVRDQSKREHTKHMLRLRRAGQLNGHQVPEIIL
                     LNSHDGSSSYQMLPGLFRGVCTNGLVCGQSFGEVRVPHKGNVVEKVIEGAYEVLGVFD
                     RVDEKRDAMASLLLPPPAQHALANAALKYRFGEDHQPVTVSQLLTSRRREDCSDDLWT
                     VYQRVQENLMKGGLSGRTAQGKSSRTRAVTGINGDVKLNRALWVMAENMLEFFGR"
     gene            5093..5536
                     /locus_tag="RHV13_003485"
     CDS             5093..5536
                     /locus_tag="RHV13_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004128347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDV1242987.1"
                     /translation="MDTQNLQFAEQTAITASVVPDELRIGFWPQHFGSIPQWITLEPR
                     IFAWMDRLCTDYHGGIWHFSTLSNGGAFMAPESEQDEKWKLFNSMNGNGAELTGEAAG
                     IVACLMVYSHHACRIECDAMTEHYYRLRDFALNHPECSAIMYLID"
     gene            5549..6091
                     /gene="radC"
                     /locus_tag="RHV13_003486"
     CDS             5549..6091
                     /gene="radC"
                     /locus_tag="RHV13_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDV1242988.1"
                     /translation="MNTVLCPESSQRELFPLTVMAQHGYLLPATSGLTPYAQRTIRRA
                     INLLDKYLRQPGISFTSSIAARDWLRLQLAGQEREVFMVLYLDNQHRLLESETLFAGS
                     VYHVQVHPREVVKSALRFNAAAVVLAHNHPSGDPEPSKVDRQMTDKLKEVLGLVDVKT
                     LDHLVVGQDGIVSFAERGWI"
     gene            6088..6309
                     /locus_tag="RHV13_003487"
     CDS             6088..6309
                     /locus_tag="RHV13_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019106756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HDV1242989.1"
                     /translation="MKIISKRQAMALYRQHPGSRLFRFCTGKYKWTGSICHYAGREVQ
                     DIRNVLAVFAERRQDRNGPYVILRSVTLN"
     gene            6335..6700
                     /locus_tag="RHV13_003488"
     CDS             6335..6700
                     /locus_tag="RHV13_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HDV1242990.1"
                     /translation="MNNHSVFGTKPENLTCQEWGLKRTITPCFGARLVQEGNRVHFLA
                     DRAGFNGAFIDDNALRLDLAFPLILKQLELMLTSGELSPRHEHCVTLYHNGLTCEADT
                     LGSCGYVYIAIYPDQPEPH"
     gene            6738..7067
                     /locus_tag="RHV13_003489"
     CDS             6738..7067
                     /locus_tag="RHV13_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HDV1242991.1"
                     /translation="MQTLSSHPTRATQPCLSPVETWQRLLTHLLSQHYGLTLNDTPFS
                     NETTIREDIDAGVSLSDAVNFLVEKYELVRIDRKGFSWQEQTPYISVVDILRARRSTG
                     LLKTNVK"
     gene            complement(7431..12017)
                     /gene="ypjA"
                     /locus_tag="RHV13_003490"
     CDS             complement(7431..12017)
                     /gene="ypjA"
                     /locus_tag="RHV13_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin-like autotransporter YpjA/EhaD"
                     /protein_id="HDV1242992.1"
                     /translation="MNRTSPHYCRRSVLSLLISALIYAPPGMAAFTTNVIGVVNDETV
                     DGVQKVDERGTTNNTHIINHGQQNVEGGVSNGSIIESGGYQDVGRHNNFVGQANNTTI
                     NGGRQSIHDGGISTGTTIESGNQDVYTGGISNGTTIKGGNSHISGGTANGTIIDGGSQ
                     RVTTQGHVDSTTINKSGSQDITQGSLATNTTINGGRQYVEQSTVETTTIKNGGEQRVY
                     ESRALDTTIEGGTQSLNSKSTAKNTQIYSGGTQIVDNTSTSDVIEVYSGGVLDVSGGT
                     ATNVTQHDGAILKTNTNGTTVNGTNSEGAFSIHNHVADNVLLENGGHLDINAYGSANK
                     TIIKDKGTMSVLTNAKADATRIDNGGVMDVAGNATNTIINGGTQNINNYGIATGTNIN
                     SGTQNIKSGGKADTTIISSGSRQVVEKDGTAIGSNISAGGSLIVYTGGIAHGVNQETG
                     SALVANTGAGTDIEGYNKLSHFTITGGEANYVVLENTGELTVVAKTSAKNTTIDAGGK
                     LIVQKEAKTDSTRLNNGGVLEVQDGGEAKHVEQQSGGALIASTTSGTLIEGTNSYGDA
                     FYIRNSEAKNVVLENAGSLTVVTGSRAVDTIINANGKMDVYGKDVGTVLNSAGTQTIY
                     ASATSDKANIKGGKQTVYGLATEANIESGEQIVDGGSTEKTHINGGTQTVQNYGKAIN
                     TDIVSGLQQIMANGTAEGSIINGGSQVVNEGGLAENSVLNDGGTLDVREKGSATGIQQ
                     SSQGALVATTRATRVTGTRADGVAFSIEQGAANNILLANGGVLTVESDTTSAKTQVNT
                     GGREIVKTKATATGTTLTGGEQIVEGVANETTINDGGIQTVSANGEAIKTKINEGGTL
                     TVNDNGKATDIVQNSGAALQTSTADGIEISGTHQYGTFSISGNLATNMLLENGGNLLV
                     LAGTEARDSTVGKGGAMQNLGQDSATKVNSGGQYTLGRSKDEFQALARAEDLQVAGGT
                     AIVYAGTLADASVSGATGSLSLMTPRDNVTPVKLEGAVRITDSATLTLGNGVDTTLAD
                     LTAASRGSVWLNSNNSCAGTSNCEYRVNSLLLNDGNVYLSAQTAAPATTNGIYNTLTT
                     NELSGSGNFYLHTNVAGSRGDQLVVNYNATGNFKIFVQDTGVSPQSDDAMTLVKTGGG
                     DASFTLGNTGGFVDLGTYEYVLKSDGNSNWNLTNDVKPNPDPNPNPNPKPDPKPDPTP
                     DPNPKPDPTPEPTPTPVPEKRITPSTAAVLNMAATLPLVFDAELNSIRERLNIMKASP
                     HNNNVWGATYNTRNNVTTDAGAGFEQTLTGMTVGIDSRNDIPEGIATLGAFIGYSHSH
                     IGFDRGGHGSVGSYSLGGYASWEHESGFYLDGVVKLNRFESNVAGKMSSGGAANGSYH
                     SNGLGGHIETGMRFTDGNWNLTPYASLTGFTADNPEYHLSNGMESKSVDTRSIYRELG
                     ATLSYNMRLGNGMEVEPWLKAAVRKEFVDDNRVKVNNDGNFVNDLSGRRGIYQAGIKA
                     SFSSTLSGHLGVGYSNGADVESPWNAVAGVNWSF"
     gene            complement(12082..12201)
                     /locus_tag="RHV13_003491"
     CDS             complement(12082..12201)
                     /locus_tag="RHV13_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDV1242993.1"
                     /translation="MPHSYAEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            complement(12356..12574)
                     /locus_tag="RHV13_003492"
     CDS             complement(12356..12574)
                     /locus_tag="RHV13_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDV1242994.1"
                     /translation="MKKLHRTSSAGNALVVCKLNRLGRSMWHLVVLLEELCERGINFR
                     ALAQSIFAQQWGDGSCKSKIICDLRFIE"
BASE COUNT         3087 a         3320 c         2950 g         3502 t
ORIGIN      
        1 ttcatcagtg aaaatataac aataagtccc tcctcctgag taaaccttac gaaaaatggt
       61 aagtcatata ataatttcac tctgatatta gttttcaaca attttgggta tttcaacagg
      121 caaacctcgc gattagttgc gatcagtcgc aactatcgcg gccagtagtg cttatatgct
      181 gcattttttg taaacggatt gttgcgaaaa taacggcctg cataaaccat tcgtgccttg
      241 cgtcttgagc ggacgatgtc tatcgcgcct tccttttcaa gtctctcaat aactctttcc
      301 agtaacttgc ctttacgtaa tacattaggc ccggcatttc gtattgtatt aaatctgaca
      361 tgatccatct ctgtttcata gtactcacga cgaagccatg ataatagcag cgcttcatag
      421 tagccctcct gagccccaaa gacctttatt gcctgttgca tgtaccattc acacagcaac
      481 caggcatttt cgacatattc tttttttatc gcgcattcac cttcagtata gtaactcagc
      541 agcgcggcca gacgtgcaac attattcgga agtttggatg caaaatcatt catgtttgca
      601 tattccctgt cggggccaat actgctttct atttcatcgt gaatttcttt ccacgccgcc
      661 aaggctgagg gctcgaatga taaacatgtc tgatgcccag tcccgtgttg agaagcactt
      721 ttttctatca gttcattcat acgttggtag aagcgattaa gattctccag cttacctgac
      781 tgaggggaag atagacgact aataaaacgc tggccctgtg tcgataattt cgcatcaata
      841 tacaagataa aacagcgtgc aaacaaacca ctcccgcgtg catgttcgcc cttgcgtttt
      901 aaaaaatcgt aaaatattgc tttttgtacc atgactaaca acgtaatacg tccattctta
      961 atcgtaaaac ttggccctgt tttccgatta acatgaaaac tttcatcatc ccatatggag
     1021 ttcataaatc ccaaatcact catagtccgt ctgctaagta tattagcccc ctcatcagtt
     1081 attagtcctg tttgtggcga gtgggtatac atttcaaact gcagggcttc aggggtgaca
     1141 ttattataaa tatacctttt ggctttcggt tgtactggct tttcagcaga taactgttct
     1201 aactgccgtg aaagtttatc tgtacatagt cctttcatag tgtctttatc aatgcatttc
     1261 agaagtgcat tttctttctt gctccatatt ttagactgga cctcatgttc tttcttctgc
     1321 ttttcatact ggagtaatac acgcagatca tgctcatgaa aaggttttaa cagcagtttt
     1381 tccaccgctg tttttctctc gccagaatct gctatcacga atgaatacaa tgacaccgga
     1441 gacttcatgg tgtctgttat ttttacatta atcaggctct gacacgccag tgataatcca
     1501 ctcagcaatg ttaatgcaat catttccccc ggggctcgtg tgcattcctc aatatcaaaa
     1561 atagtttcaa gcaatgacga ggggaaagcg ttaaatggaa actcctgcat gacaatacct
     1621 ccagaaaatg ttgttttgat aattgaaaac taatctggaa tgcgatcgca agtcaatatg
     1681 aaaaactgat atttagtcta gtcagacagc gttggactaa attagtcaag tggcagcttc
     1741 tggtattttc attcgcctgt gctttcttta taaaaaaaca gacgagtaat gtcagtacgg
     1801 agatatactg ccaggtaata tgatttaaat aattctcaga tatatataac ctcagtgaat
     1861 ttccacccgt tttaatccaa caggtcatcc ccaccggatg gccttttttc attcagaggc
     1921 cgatatgaaa catccctcgg gttataccct tatccgtcgc catctgcggc gttatccaca
     1981 cgcgctacgc cagcatttgc tgcaggagct caaccgactc gttacgtatg aaccggtgat
     2041 tggcatcatg ggaaaaaccg gcgtcggcaa atcctcgctg tgcaatgcac tgttccggag
     2101 cgaagtctgt actgtcaatg ccgtggaagc ctgcacccgt cagccacagc gtgtccggct
     2161 gcgcttcggc agccattatc tgacgctggt cgacttgccc ggagtgggcg agtctgttac
     2221 ccgcgacggt gaataccgtg agctgtatcg tgacctcatg ccacagctcg atatggtgct
     2281 gtgggtgctc aaggccgatg accgcgcatt tgcggtggaa gagcaatttt atcaggacgt
     2341 gttcgcgcag ttcagaggac cgataccgcc agtgctgtgg atactcaacc aagtggacaa
     2401 aaccgaacct gccgagcagt ggcgatggtt gtcggctcaa ccctcggcgt tacaggcgga
     2461 gcgtattgcg cagaaacggc aggctgtggc acgtcagttg cagatagcag aaatagatat
     2521 tctgccagtc tctgtccggg ggcgttaccg gctgccgcgc ctggtggaga ccatgattac
     2581 ccgcctccca aagcaagccc gcagtccact tgtgcctcat ctgcagaacc ggtatcgcac
     2641 tgataccgtt atcaataccg ccagcagcag ctttggcgat gccgtggtag aggtgattga
     2701 taacatcatt gacctcgcac cgctaccgca ggtggcccgc acagcactgc atgcggtgac
     2761 ccacaccgtc gcccgcgcgg caggctccct gtggggattt ttcttcggct agtcacttcg
     2821 ctttcgatga gcggccttgc cgttttcgat aagtttcttt gtttcattac gatcgatttt
     2881 gtcgatcgag ccgatcgata ttcatcgttg tacatgatta atccggtagc cacataatcc
     2941 acagggcatt atgtgatttg ttaattttaa ccatcaaaat caatgcgatg tatcgcaggg
     3001 attgtcacgc attaaggaaa gtatatgccg ttaattctgt tatggggcgg tctggccctg
     3061 ttgctgggta tcgtggcttc cgctaatgga cgctcgttct ggggctggtt cattctcggt
     3121 ctgattatcg acccgatact ggcggggctg ctgtactggc ttattgcaaa ggaccgcaca
     3181 tgaaaacatt tctgcgtctg ttcggtaagt tcacccgctg gtacatcacc ctcattatct
     3241 ttatggcctg cctactgctc attatcacca ccctgggcaa tatggctggc ttctgacagg
     3301 acactccaat gaaaaataaa ctggttattt catttttact ggcttcagtg ctgacgcctt
     3361 ctgtctcgca tgcgtttggc aaccctgata gctgggtaag tggctatgcg cagggaacgt
     3421 ctgaatacac catcctcggt aaagggcaat cacaacttta tctggcctgc gatagttccg
     3481 gctcacagcc agcgaccatt atcttcactg acgtgaacgg ccaacaggtc aggatggaca
     3541 gcggccagac acttgccatg agaattgata acgctgaaga agtgaatatc agcgaaagcg
     3601 agtctcatgt gggatctgac aatgtgatgt gggcatggaa caaattacgc accggcaaac
     3661 gtgttgtggt ttcaggtagc ggggtaaaac ctgccacttt cactttagcg ggggctgctg
     3721 cagttcttcc tgccttcggt gacaacggct gtgtgcccgg atttgcactg tagctctgcc
     3781 tcactcttac agattaaagg aaatttaatg aagaaaatta ttacagtcgc agcactcagt
     3841 ctgctgtcat tttcttcgct ggccgggtct tcacctgtta gcgtcagcgt cagcccggga
     3901 agctacagtc actattcatc gattcgggtt acatcaaaag tcgattcgat tgtgattaaa
     3961 caactcattg tcaacagggg aaactgccag gatgcagaat tcgccagccc ctggaaaccg
     4021 gtacgtctgg gatttggcgg tgctgtatcc catgaattta ccggcaaggg gctgatggtt
     4081 ccctgtaatg tactggaagt agttgtccag acaagcgaag gcgcatggca gtttgatttt
     4141 gacagctgac accgcgcgct tttccacctt tcttttctat ctgaacgccc tgctcaaacg
     4201 agcagggcgt tttcttttgc ctctcttaaa ggaattactt atgagattag ccagccgctt
     4261 tggccgtata aaccagatac gccgtgaccg tcctctgacc catgaagagc tgatgagtca
     4321 tgttcccagt gtattcggga gtgacaaaca tgaatcgcgt tcagaccgtt acacctacat
     4381 tccgaccatc accatcctcg aaagcctgca gcgcgagggt tttcagccgt tctttgcctg
     4441 ccagacaaag gttcgggacc agagcaaacg ggagcacacc aaacacatgc ttcgcctgcg
     4501 tcgcgccggg cagctaaacg gtcatcaggt accggaaatc attttactca acagccatga
     4561 cggctcatca agctaccaga tgctgccggg gttgtttcgt ggcgtctgta ctaacggtct
     4621 ggtctgcggt cagtcattcg gagaggtgcg ggtaccacat aaaggtaatg tcgttgagaa
     4681 ggtaattgaa ggggcttacg aagttctcgg ggtatttgac cgggtggatg aaaagcgtga
     4741 tgccatggcg tcacttttac ttccgccacc agcacagcac gctctggcga atgccgcgct
     4801 gaaatatcgc tttggtgagg accaccagcc ggtcacggta tcgcagttgc tgacctcacg
     4861 tcgccgggag gattgcagcg atgacctgtg gaccgtctac cagcgcgtgc aggagaacct
     4921 gatgaaaggg ggattgtcag gacgaaccgc acaggggaaa agcagtcgca cccgtgccgt
     4981 taccggtatt aatggcgacg tgaagctcaa tcgtgccctg tgggtaatgg cagagaatat
     5041 gctcgagttt ttcgggcgtt aaacaacgat acaagcataa ggagatagtt tcatggatac
     5101 acaaaacctt cagttcgctg agcagacagc aattactgcc tctgtggtac ctgacgagtt
     5161 acgcatcggc ttctggccac agcactttgg ctccatcccg caatggataa cccttgagcc
     5221 cagaattttt gcctggatgg accgtctgtg taccgattat cacggtggta tctggcactt
     5281 ttcgacgctc agcaacggcg gcgcttttat ggcccctgag tcagagcagg atgagaagtg
     5341 gaaattgttc aacagtatga atggcaacgg cgcggaactt actggtgaag ctgcagggat
     5401 agtcgcctgt ctgatggtat acagccatca tgcctgccgt atcgaatgtg acgcgatgac
     5461 agagcactat tatcgcttac gagacttcgc gctaaaccat ccagagtgca gtgccattat
     5521 gtatctaatc gactgaggga tacctgacat gaataccgta ttatgcccgg agtcttcaca
     5581 gcgcgaacta tttccgctga cggttatggc acaacatggt tacctgctcc ctgcgacttc
     5641 cggattgaca ccctatgccc aacggaccat tcgccgggct atcaatctgc tggataaata
     5701 tctgcgccag cccggcatat cctttacatc aagtattgct gcccgtgact ggcttcggtt
     5761 acagctggca ggacaagaac gggaagtgtt tatggtgctc tatctcgata atcagcaccg
     5821 tttactggag agcgaaacgc tgtttgccgg ttcggtttac catgtccagg tccatccccg
     5881 tgaagtggta aagtctgcac ttcgcttcaa tgctgccgct gtcgtattgg cgcacaacca
     5941 tccatccggc gatccggagc ccagcaaagt cgatcggcag atgactgaca aactgaagga
     6001 ggtgctggga ctggttgatg tgaaaacgct cgatcacctg gttgtcggtc aggatggcat
     6061 tgtgtcgttt gcagaacgag gctggatatg aaaatcatca gtaagcgcca ggccatggct
     6121 ctgtaccggc aacatccggg ctcccgactg tttcgcttct gtaccggcaa atataaatgg
     6181 actggcagta tctgccatta tgctggccgg gaagttcagg acatacgcaa cgttctggcc
     6241 gtctttgctg agcgccgtca ggatcgtaat ggtccctatg tcattcttcg aagtgtcact
     6301 cttaattaat ccttcccttt aatcaggaac aataatgaac aatcactcag tattcggtac
     6361 caaaccggag aatcttacct gccaggagtg gggactaaag cgcacgatca ccccctgttt
     6421 tggcgctcgt ctggtgcagg aaggtaaccg ggtgcatttt ctcgctgaca gggccggatt
     6481 caacggtgct ttcatcgacg ataacgcgct acgcctggat ctggcctttc cgttgatact
     6541 caagcaactg gagctgatgc tcaccagcgg agaactctct ccccggcatg agcactgcgt
     6601 cacgctatat cacaatggac tgacttgcga agccgataca ctcggctcct gtggctatgt
     6661 ctacatcgct atttatcccg accagcctga accccattaa ctctacgctt atctcttaaa
     6721 ctcatagagc aaacatcatg caaaccttat catcacaccc gacaagggcg actcagccct
     6781 gtctgtcacc cgttgaaacc tggcaacggc tgctgactca tctgttatcg cagcactatg
     6841 gcctcacgct gaatgacaca ccgttcagta atgaaaccac catacgtgaa gatatagatg
     6901 ccggagtatc gctcagcgat gcggtgaact ttctggtcga aaaatacgaa ctggttcgaa
     6961 tcgaccgaaa gggattttcg tggcaagaac aaaccccgta tatttccgta gtggatattc
     7021 tgcgagcaag gcgctctacc ggcttgctaa aaactaatgt gaaataaacg cttaaataca
     7081 gagcagactt aaggaaagca aaatgctaat ctcacagacg aagagactcc tgctgtaacc
     7141 tcccctccct gcaaaatacc tgacattttc ttttaggacc aacaatggga ccaaaatgaa
     7201 aattgaactg aaggttatca gccattaaac aacaagttac aaacccaatt cagactccgc
     7261 cagcccacca atcatgattg gacggtgtaa ggacaacacc aacaaaaaca ggaagttagc
     7321 agtctcagca ggacaccgac cagacggtga ggagacaaaa aaggatacgc aaaggagccg
     7381 cggctcctgg cgacatgaaa gcccacagat gtgggctttt tcgttgatgg tcagaacgac
     7441 cagttcacac cagccaccgc gttccacggg gattccacat cggcaccgtt gctatacccc
     7501 accccgagat gcccgcttaa cgtactgctg aatgaggctt taatacctgc ctggtatatt
     7561 ccacgtctgc ccgacaaatc attgacgaaa ttaccgtcat tattcacctt cacccggtta
     7621 tcatcgacaa attctttgcg cacagccgcc ttcagccacg gctcaacttc cataccgttc
     7681 cccagacgca tgttgtaact cagcgttgca cccagttcac gatatatact gcgggtatcg
     7741 actgatttcg attccatgcc attggataaa tgatattcag ggttatcagc ggtgaacccc
     7801 gttaacgatg catacggcgt caggttccag ttaccatcgg taaatcgcat cccggtttca
     7861 atgtgaccgc ccagcccgtt gctgtggtaa ctgccattgg cggctccacc gctgctcatt
     7921 ttaccggcta cgttactttc aaaacggttc agcttcacga caccgtccag atagaaacca
     7981 ctttcatgtt cccaactggc atagccgccc agagaataac tgcccacact gccatgtcct
     8041 ccgcgatcaa aaccgatatg tgaatgggaa taaccaataa aagcgcccag cgtggcaatt
     8101 ccttcaggaa tatcattacg gctgtcgatc cccactgtca ttcctgtcag cgtctgctca
     8161 aacccggcac ccgcatcggt ggtgacatta ttacgggtgt tatacgtcgc cccccagaca
     8221 ttattgttgt gtggactcgc tttcattatg ttcaaccgct cgcgaatact gtttagctca
     8281 gcatcaaata ccaacggtaa tgttgctgcc atattgagta cggctgcggt agaaggcgtg
     8341 atgcgtttct ccggaacggg tgtcggcgtt ggctcgggag tcgggtctgg ttttggattt
     8401 gggtctggcg ttggatcggg ttttgggtct ggcttcggat ttggatttgg gttggggtcc
     8461 gggttgggtt tgacatcatt ggtcaggttc cagttgctgt tgccgtcgct tttcaggaca
     8521 tactcatagg tcccaagatc aacgaaaccg ccggtattgc ccagcgtaaa cgaagcatcc
     8581 cctccccctg ttttcaccag cgtcatcgcg tcgtcagact gtgggctgac gccggtatcc
     8641 tgaacaaaga ttttaaaatt accagtggcg ttgtagttga cgaccagttg atcgccccgg
     8701 gagcctgcaa cgttggtatg caggtagaaa ttaccgctac cggaaagttc attggttgtc
     8761 agcgtattgt agataccgtt agttgtggca ggcgctgctg tttgtgctga taaataaaca
     8821 ttaccgtcgt taagtagcaa actgtttact ctatactcgc agttgctggt gcctgcacag
     8881 gaattattgc tgttaagcca gacactgccc cggctggcag ccgtcaggtc ggcaagcgtt
     8941 gtatcgacgc catttcccag agtcaatgtc gcgctatcgg taatccggac cgccccttcg
     9001 agtttaactg gcgtaacatt atcccgtggc gtcattaacg acaggcttcc tgtcgcgcca
     9061 ctgaccgatg catccgccag cgtacctgcg tagacgattg ccgtcccgcc agcgacctgg
     9121 agatcttctg cccgggccag agcctgaaac tcatcttttg accgcccaag ggtatattgc
     9181 ccaccagagt taacctttgt ggcggagtcc tgacccaggt tttgcattgc cccacccttg
     9241 ccaaccgtgg agtcgcgagc ttcggtacct gctaatacca ataaattacc gccattttcc
     9301 agcaacatat tggtcgctaa attgccggaa atggaaaaag tgccgtactg gtgagtaccg
     9361 ctgatttcaa taccgtcagc cgtgctcgtc tggagagcgg caccgctgtt ctggacgata
     9421 tctgtcgctt tgccattatc gttgactgtc agcgttccgc cttcattgat ctttgttttt
     9481 attgcctctc cgttagctga aactgtttgt attccgccgt cgttaattgt tgtctcattc
     9541 gccacaccct cgacaatttg ttcaccgccg gtgagcgtcg tgcctgtcgc agtggctttt
     9601 gttttgacga tctcccgtcc gcccgtattg acctgtgttt tggcggaagt ggtgtctgac
     9661 tccacggtta acacgccgcc atttgccagc aggatattgt tcgccgcacc ctgctcgatg
     9721 ctgaacgcga cgccatccgc gcgtgttcct gtgacccgcg tcgccctggt ggttgcaacc
     9781 aaagcgccct ggctactctg ctgtatcccc gttgcgctgc ctttctcccg cacatcgagt
     9841 gtgccgccgt cattaagcac cgagttttca gccagaccgc cctcattaac tacctgtgaa
     9901 cccccattaa taatggaacc ttccgctgtc ccgtttgcca taatttgttg taggccagag
     9961 acgatatcgg tattgattgc cttaccataa ttctgaacgg tttgcgtgcc accattgatg
    10021 tgtgttttct ctgttgaccc gccatcaaca atttgttcac cactttcgat atttgcttca
    10081 gtggctaaac catataccgt ttgcttgcca cctttgatat ttgctttatc agaagtggca
    10141 ctggcatata ttgtttgggt gccagcacta ttgagtacag tgccaacatc ttttccataa
    10201 acatccattt tgccgttggc attaataatc gtgtcaactg cccgggaacc agtgacgact
    10261 gttaatgagc cagcgttttc cagcactaca tttttagctt ctgaattcct gatgtagaaa
    10321 gcatcaccat aactgttggt tccttcgata agtgttccgg aggtcgtgga agcaattaat
    10381 gcgccgccgg attgttgctc aacatgctta gcctcaccac cgtcctgaac ctccagaacg
    10441 ccgccattat taagtctggt gctatctgtt ttagcctcct tctggacaat cagcttaccg
    10501 ccagcatcaa tggtagtatt tttcgccgag gttttagcca ctaccgtcag ttcgccggta
    10561 ttttccagca caacataatt agcctcccct ccggtaatag tgaagtgaga gagcttgttg
    10621 tatccttcga tatcagtccc tgcacccgtg ttggcaacta aagcactgcc cgtctcctgg
    10681 ttaactccat gtgcaatacc gccggtatag acaatcagtg agcctccggc gctaatattg
    10741 ctgccaattg ccgtaccatc tttctcaaca acctgccggc tcccggagga tataattgtt
    10801 gtgtcagctt tcccgccgct tttgatattt tgcgttccgc tgttgatatt agtgcctgtg
    10861 gctatgccat aattattaat attctgtgtg ccaccattaa ttatggtatt tgtcgcgttt
    10921 ccggcaacat ccataacccc gccattatct attcgggtcg catcagcttt agcattggtt
    10981 aaaactgaca ttgttccttt atctttgata atcgtcttgt ttgccgaacc atatgcgttt
    11041 atgtctaaat gaccaccgtt ttccagcaac acattgtctg ccacgtgatt gtggatggag
    11101 aatgcacctt cactattcgt accgttcacc gtcgtaccgt tagtgtttgt ttttaaaatt
    11161 gcaccatcgt gctgggtaac atttgttgcc gtaccaccac taacatcaag cacgccaccg
    11221 gaataaactt caataacatc cgaggtgctg gtgttatcaa caatttgcgt gccaccagaa
    11281 tagatctgcg tattttttgc cgttgactta ctattcagag actgagttcc gccttcaatc
    11341 gtcgtgtcca gcgcacggct ctcatatact ctttgctcac cgccattttt aatggttgtt
    11401 gtttctactg tgctctgttc aacatactgt cgaccaccat ttatggttgt gttcgttgcc
    11461 agacttcctt gcgtgatgtc ctgagagcca gacttattta tcgttgtact gtcgacatgc
    11521 ccctgagttg ttactcgttg gctgccacca tcaatgattg ttccattcgc agtcccccca
    11581 cttatgtgtg aattaccacc cttaattgtc gttccattgc tgataccacc tgtataaacg
    11641 tcctgattgc cactctcgat tgtcgtacct gtggaaatac ccccgtcatg aattgactgt
    11701 ctgcccccgt taatggttgt attattagcc tgccccacaa aattattatg ccttcctaca
    11761 tcttgatatc cacccgattc aataatactt ccattggata ccccgccttc aacattctgc
    11821 tggccatggt tgataatatg agtgttattt gttgtacctc gttcatctac tttttggaca
    11881 ccatctacag tctcatcgtt taccacacca ataacattag tagtaaaggc cgccatcccg
    11941 ggcggggcat atatcagggc agatatcaat aaggaaagta ctgagcggcg acaataatgg
    12001 ggactggtcc tgttcataaa tttcatcctc tgaaaagtga atactgagta gcgtttaagc
    12061 gaccttagct ttgctgcaac atcagcccac aggcaccaga ccaggggatt catcctgaag
    12121 agacagcgca agtgtattgt gttcaccgct catcaaagac atcatgatga aatgatgata
    12181 ttccgcataa gaatgaggca ttttttaaac gcagtgcgct gaagtgtggt tggataaaaa
    12241 agtcaatcta ttcaggaaac acgggcgtat tcttttcttt cgacaatcag gcagtcggca
    12301 aaataaaatg atttacataa tcgtttctga tgaatatctt ctgctcacat aaaaatcact
    12361 caataaatct gagatcgcag attattttac ttttacagct tccgtccccc cattgttggg
    12421 caaatataga ttgggccaga gcacgaaagt taataccacg ttcgcacagc tcctccaaca
    12481 gcacgacaag atgccacata ctgcgcccca gtcggttcag tttacagact accagtgcgt
    12541 tccctgccga tgatgtcctg tgcagttttt tcagaccgga cctcctgcca cagttttacg
    12601 ttgaagtggt aaccctgagc acgcagttct tcagtcaggc gtggtgcacc gtaacgctgt
    12661 tattgctggg taagatcaaa aaactttcag gcagctaagg aaagttgaac cagacattag
    12721 atgaaatatt tcaaccaatt acagcaccaa ttcagtcact gccagcccac caaataaatc
    12781 aaggggttac atgaaaacgt agcccctttt tctttggtag tgacactaaa atggatgtag
    12841 tgtgaagaat aatcccatt
//
LOCUS       DAPGOF010000137        12830 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.138, whole genome shotgun sequence.
ACCESSION   DAPGOF010000137 DAPGOF010000000
VERSION     DAPGOF010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12830
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.138"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..304
                     /locus_tag="RHV13_003493"
     CDS             <1..304
                     /locus_tag="RHV13_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDV1242995.1"
                     /translation="NVNFNNANLSNCHFNCSVLTKAWMFNIRLYRVNFDEASVLGMGI
                     TILRGEENISINSDILVTLQKFFEEDCTSHTDMSQTEDNIHAVAMKITADIMQDAD"
     gene            complement(458..2107)
                     /locus_tag="RHV13_003494"
     CDS             complement(458..2107)
                     /locus_tag="RHV13_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HDV1242996.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(2259..3608)
                     /gene="ghxP"
                     /locus_tag="RHV13_003495"
                     /gene_synonym="yjcD"
     CDS             complement(2259..3608)
                     /gene="ghxP"
                     /locus_tag="RHV13_003495"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HDV1242997.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(4154..4618)
                     /gene="soxR"
                     /locus_tag="RHV13_003496"
     CDS             complement(4154..4618)
                     /gene="soxR"
                     /locus_tag="RHV13_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HDV1242998.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            4704..5027
                     /gene="soxS"
                     /locus_tag="RHV13_003497"
     CDS             4704..5027
                     /gene="soxS"
                     /locus_tag="RHV13_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HDV1242999.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(5030..6616)
                     /gene="pdeC"
                     /locus_tag="RHV13_003498"
     CDS             complement(5030..6616)
                     /gene="pdeC"
                     /locus_tag="RHV13_003498"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HDV1243000.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     SIKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            7046..7327
                     /locus_tag="RHV13_003499"
     CDS             7046..7327
                     /locus_tag="RHV13_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HDV1243001.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            complement(7426..7962)
                     /gene="ssb1"
                     /locus_tag="RHV13_003500"
     CDS             complement(7426..7962)
                     /gene="ssb1"
                     /locus_tag="RHV13_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HDV1243002.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            8217..11039
                     /gene="uvrA"
                     /locus_tag="RHV13_003501"
     CDS             8217..11039
                     /gene="uvrA"
                     /locus_tag="RHV13_003501"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDV1243003.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(11074..11430)
                     /locus_tag="RHV13_003502"
     CDS             complement(11074..11430)
                     /locus_tag="RHV13_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDV1243004.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(11434..11850)
                     /locus_tag="RHV13_003503"
     CDS             complement(11434..11850)
                     /locus_tag="RHV13_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HDV1243005.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(11961..12674)
                     /gene="aphA"
                     /locus_tag="RHV13_003504"
     CDS             complement(11961..12674)
                     /gene="aphA"
                     /locus_tag="RHV13_003504"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HDV1243006.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     SMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         3315 a         3367 c         3165 g         2983 t
ORIGIN      
        1 taatgttaat tttaataatg ccaatttaag caattgccat ttcaactgtt ctgttttaac
       61 aaaagcctgg atgtttaata tccgtctcta tcgtgttaat ttcgatgagg ctagcgtcct
      121 gggaatgggt attaccattc tccgtgggga ggaaaatatc tccattaata gtgatatcct
      181 ggtaacacta cagaaattct ttgaagaaga ttgtacctct catactgaca tgtcacaaac
      241 tgaggataat attcatgcag tcgctatgaa gattactgca gatattatgc aagatgcaga
      301 ttgaggttaa gccgaagaac agccaatgaa ttgactttaa aagagagata acttatttgt
      361 tttagaatca tgaatgttag cccgagctaa cctgttaaag gtgcaaaaga ttgcaccttt
      421 ttcagattac ctcgggcctg gttacgtcgc tttcggctta ctgattttcc tcaattagca
      481 acgcttcaag caaatcgaga tcgtgcagta atttttgcag cgtttcgttg ctgatctccc
      541 gcgtggcgcg caggtggtac agttcagcac gttcagaacg caatgccgcc agacggaagc
      601 gacgctcaag gttctcttcc tgcacggaac tttcaacgtc gttacgtcca tcggcgcgac
      661 gacgcaggtt accaatgacg cgagaactga cctccgtaag cagctggtta tcgatattct
      721 cttcggtatc cgccgccaga cgctcctcca ttttctggat cgccacaatc gccacttctg
      781 ccgttgccgc tcgcgcaata cgttcttctt tcaattgttg cgaatggtcc gcgacttcaa
      841 tgtgttgtag cagaataggc aacatcacca cgccgacaaa cagcgagaag agaatgacgc
      901 cagccgccag gaacaccagc tcatagcgcg ccgggaagac gttaccatcc ggcaagagca
      961 gcgggataga gagcacaccg gccagagtga tcgccccacg caccccggcg aaagacgcga
     1021 tcaggatttc tcgtgtggtc cacgaaccaa actccatcgg cttcttcttc aggaagcggt
     1081 tgctgaactt tttcatcgtc cacagccagc cgaaacggac cagcatcagc gccgcatata
     1141 tcagaataat atcggtaaac agcatccaga tttcgacgtt agggtcgatt tctgccgcca
     1201 tcagagacgt ctccagaata cccggtagct gcagacctaa cagcaggaac accatgccgt
     1261 taaagacaaa ttccagcatc gcccaggtgc tgtttgcgcg caggcgcatt gccagtggcg
     1321 cacggcgcat cacaccggag cgggtgatgg tcatcccggc ggcaaccgca gcgaggatgc
     1381 cggaaacgcc aatatgttcg gcaatcagat aagaagcgaa tggcagcaag aacagcagaa
     1441 caatctgcgt cgcgggttca tcaccgcccc agcggctgag gaatcgcagc gagcgaccgt
     1501 acagccagct caccacaaaa ccggcgagaa taccgccaat ggctactttc ataaattcga
     1561 ccgtcgcacc gccgacggtg aagatcatcg tccccatcgc cactgccacg gcaaacttca
     1621 acgacaccag gccggaagcg tcattcatca acgcttcgcc ctgcaaaatg cccatgattt
     1681 ttttcgggat gcgcccttcg ccaacaatcc cggagagcgc cacagcatcg gtcggagaaa
     1741 gtaccgccgc cagcgcaaaa gcggggatca gcggaatgcc cggcaccacc cagtaaataa
     1801 ggaagccaat gccgaccacg gtgaccacca ccagcgccag cgcgaggccg aaaatctctc
     1861 gaccatgttc gagaaattca cgggtcggcg ttttccagcc atcagcgaac agcaacggcg
     1921 ggataaacaa gactaaaaag agttcaggat caaactccac atgcaaacca aacgtcggcc
     1981 acgccagtag cgcaccgatg gcgatttgca taagcggaag cgggatctga aagggcatga
     2041 cacgagtgac caccccggac agcgagacca cgagggtcat tatcagtatg gtgaagaaga
     2101 tttccatgcg ttccctgttt gcgatgtttt tttatgatct gagaaaggcg ttatgcctga
     2161 attctcattt tttcagaata tcgcaaaagc tcacacaatg tgcggagaaa ataaaaacgg
     2221 gcagccattg ccacccgttt tcagaatccg atgaaagatt agatagccca gccacccgca
     2281 tagaaggtca ccagcgccac ggcgataacg accgtaccga tgttcaactt gcgccattca
     2341 ccggaaacca gacgaccaat caccagagtc gcgaagccga tcatgatgcc tgttacgatg
     2401 ttacaggtca gcacgatgaa taccgccgta accagacccg ccatcgcatc aacaaaatca
     2461 gcaaagtcga ttttcgccac gttgctcagc atcagcaggc caacgtacat cagcgccgga
     2521 gccgttgcat accccggaac gaggtaagaa agcggagaga ggaacagaat cagcaggaac
     2581 agcacgccaa cggtgatagc cgtcaaaccg gttttaccgc ccgccgccgt acccgccgca
     2641 gactcgatgt ataccgctgc cggagctgca cccaccagac cagagaaaac gctgctcatg
     2701 gagtcagtgg tcagtgcttt cccgccgtcg atgatctgcc catctttatc cagcaggttc
     2761 gcctggccgg cgacggcacg gatagttccg gtggcatcaa atactgccgt catcaccagc
     2821 gccagaacgc ttggcaggac tacaggattc agcgcgccca taatgtccag gctgccaatc
     2881 agtgaattgc cgttttcatc gctcaatgaa ggcatggcga aaacgccgga gaaatggacg
     2941 ttaggatcga agatcaaacc gacaattgag ataccgataa tggtcagcag aatgccacca
     3001 gggactttca gtttttccag gccgatgatc accgccagac ctaccagtga cataatcacc
     3061 gggaaggtcg cgaaatcacc cagcgcaacg ggcagaccat caagcgggtt tttaatcacc
     3121 agaccgacac cgttagcggc aatgagcagc aggaacagac cgataccgat ccccgtgccg
     3181 tgcgccacac cgtgcggcaa gttgcgcaaa atccagctac ggatacccgt ggcagaaatt
     3241 accgtaaaca gcacacccat caggaacacg gcacccagcg cgacaggtac gctaatatgt
     3301 tgccccagca ccaggctgaa tgcggtaaac gccgtcaggg agatggcgca accaatcgcc
     3361 aacggcagat tagcccacag acccatcacg atagaaccga gtccggcaac cagacaggtc
     3421 gcaacgaaaa ctgccgcagg cgggaagccc gctttaccca acatgcctgg aacgacgata
     3481 accgagtaga ccatcgccag aaacgttgtt aacccggcaa ccacttcctg acggacagtg
     3541 cttccacgtt gtgaaatttt aaaccaggcg tcgagtgaac cgccggtacg cgctgatggc
     3601 gtagacataa gagaacatcc cctgagagtt taattttcgt caagatgcgt ggcggacaat
     3661 cccctgccag cgaaacgtta tcttcctgct ccaggtttgc gacaatttgc tgcgtcgcaa
     3721 aaaagcaaac gtttagctcc gcgcatacaa aacgggtggt aaaaaaagca aacgattatc
     3781 cggcgatcat agcggctttt tcaaccgaag tttgctgctt ttttctcttt tattgtcttg
     3841 acgatgaaaa aacaggcaat tgatgcgcaa acgttatcgt cggtgcgcaa gattgcagcg
     3901 ttatcgagcc gtataccgtt caggattgcg ataaaaaatc gtctgagatg acaaaaatat
     3961 tgcggttaaa aagtatactt ttacactggc gaagaaacgt cggtgacata caattaaagc
     4021 atcaacacca accggaacct ccaccacgtg ctcgaatgag gtgtgttgac gtcgggggaa
     4081 accctcctgt gtaccagcgg gatagagaga aagacaaaga ccggaaaaca aactaaagcg
     4141 cccttgtggc gctttagttt tgttcatctt ccagcaagcg tgcgccggta ccttcttctc
     4201 ctaagcggtc gcccgggtta cgcaacgggc aatcactgcg cgaaaggcag ccacaaccaa
     4261 tacatccgtc cagttcatca cgcagcgcca ctaaggtatg aatgcgccga tccaactctt
     4321 ctcgccattg ggacgaaagc tgtttccact ctttcgcact taacgtatgc ccttcgggca
     4381 acacgccaaa cgcttcacca atggtcgcca gcggaatgcc aatacgctga gcaattttga
     4441 taattgcaac atatcgcaac acatcgcgtt tatatcgccg ctgattgccg ctgttacgga
     4501 tactggtaat caacccttta ctttcataga aatgcagcgc cgataccgcc acaccgctgc
     4561 gtttcgccac ttcgccgggg gttagcagcg ctttaatgcg gggtaatttc ttttccataa
     4621 atcgctttac ctcaagttaa cttgaggaat tatactcccc aacagatgaa ttaacgaact
     4681 gaacactgaa aagaggcaga tttatgtccc atcagaaaat tattcaggat cttatcgcat
     4741 ggattgacga gcatattgac cagccgctta acattgatgt agtcgcaaaa aaatcaggct
     4801 attcaaagtg gtacttgcaa cgaatgttcc gcacggtgac gcatcagacg cttggcgatt
     4861 acattcgcca acgtcgcctg ttactggccg ccgttgagtt gcgcaccacc gagcgtccga
     4921 tttttgatat cgcaatggac ctgggttatg tctcgcagca gaccttctcc cgcgttttcc
     4981 gtcggcagtt tgatcgcact cccagcgatt atcgccaccg cctgtagttt tattgcccgc
     5041 gcgttaactc ccgcgcgggt aattgctcca tccattgcat aaacacctgc ggcggcatcg
     5101 ccttcgcaaa aaaccatccc tggcaatagc gcacgccgcg tttgcgcagc cagttaacct
     5161 gctcctcagt ttcgacgcct tcagcgatcg tttttaaccc caggctgtgc gccagctcga
     5221 tgatgtgttc cgcaatcaaa tgactggttt tgtgggtggt cagcgtttca acaaacgatt
     5281 tgtcgatttt caaaatatcg acattcaatg atttaaggtt atgcaagtta gagtagccaa
     5341 taccaaaatc atcaattgcc acttcgtaac cagcctggcg gaaagccaga ataatcggcg
     5401 tcattttttc gacatcaaga aatgcatgct cagtcacttc aaatttaatt tgctgcggac
     5461 gcaccgcgta ttgctctgtt ttctgattga ttcgcgctat caaccgtgac gtatgaaaat
     5521 cggaggccga caggttaata gaaacatagc gatcggcatg tgttgccaag tacgcgccca
     5581 gatcgcggaa gacattatca ataacataat cagttatctg ttctatcatc ccctcctttt
     5641 ctgccagcgg aataaactct gccggattca ttatttgccc ctgctcacca agccaacgta
     5701 acaacgcttc agcgccgata catttttctg ttttgatatc gattattggc tggtaataaa
     5761 gacaaagttg atgtttttcg agggcgcgtt gcaatttacg tttgggagat aaatagtttt
     5821 gtcgaatacg tagccagagt aatagcagaa caagactccc caaaataccg gcgggcaacg
     5881 caaaaataag atgattataa aaatgggtta taagacgttg atatgaagtc gcaacaatgg
     5941 ctgcaattgg gcgttttatc gaataaactg tcgcatataa atagccattt ctttgtacgg
     6001 ttaaatcctt caaatgaatc agcggagaaa acgttgctgc cgaggcctct ttgcttaacg
     6061 agaaaaaggt tttcgtcacc gtatcataca caccccattg caatgtcgga tcatcagaca
     6121 tcacttcact ccagaagaga gggttgataa ccgccacata atttccccgc tgcatatagg
     6181 tcattttata gccagagaaa aaaggcgtat cgcggtaata atagatagaa acgttaggtt
     6241 cacgcttata atcggccggt gcaatcgtat agccgtttac aggcgctatc agcgatgagc
     6301 ataaaaaatg gttatcacgg gcatagatca attcattaat ataaagatag ccacgaataa
     6361 tattcaacat tcgcttttga tgggctggag tgcaaacttg cccctgatag cgttcagctg
     6421 catcgctcac taaatctgcc tggcgaatga ccagctcgga tttatccagt gccagttgag
     6481 caaaggttcg gagctgatta ttcacttctg attttgccca aaggaaggca atccatagcg
     6541 aaagaatgat gggaaagaga actaaaaaga taatgcccgg caacgccagt aattggtgtc
     6601 gtgcacgatg actcattgct atcaatttcc tgcctgttta tacccggcca ttgtaaggtg
     6661 aaaaccctgg gtaccgacgt ctttagataa atcttacggt ataaatagca cttttgcctg
     6721 atttgaaatt gatacctttc taatcgtcac ctggatgaaa ataattcatt gatattcaaa
     6781 attaatttcc tacaggaatg cagaggcggc gggaagatat acccttaatg ccactgaaaa
     6841 accacattat ctgtacagca ataaaagtca cgtcctaata tggctaacaa acaatcaatt
     6901 gtgatatagt tcacaaaatt aatgaaacaa acagagtgtt tcatttttgt gttctgcaag
     6961 ccccgcgata gccagagtgt accacgcctc ccgtgaacaa cgccgcgctg tccagggcat
     7021 cggctctttt cagaggaatt gtttgatggc taccctcacc actggcgtgg ttcttcttcg
     7081 ctggcaactt cttagtgccg taatgatgtt tctggccagc acgctcaaca tccgttttcg
     7141 tcggtctgat tatgtcgggc ttgcagtgat cagcagcggt ctgggcgtgg tttccgcctg
     7201 ctggttcgcg atggggttgc ttggcatcac aatggcggat atcaccgcca tctggcacaa
     7261 catcgagtcg gtgatgatag aagagatgaa tcagacaccg ccacaatggc caatgatttt
     7321 gacctgatac gtagaagcct ccataacgga ggcttctttt ttatggctgg cagatgcttt
     7381 aatcatccac cttaaaacaa tataacctat tgttttaatg acaaatcaga acggaatatc
     7441 gtcatcaaag tccatcggcg gctcgttaga cggcgctgcc ggagcggact gctgcgggcg
     7501 agactgcgcg ccgccgctga actgattgcc accctgcggc tgctgaggct gaccccaacc
     7561 gccctgcggc tgaccaccac cgatattgcc acctgcagga gcaccaccac cctgacgacc
     7621 acccagcatc tgcatggtac cgccaacgtt caccacgact tctgtggtgt agcgatcctg
     7681 accggattga tcggtccatt tacgggtacg cagctgacct tcgatataaa cctgagaacc
     7741 tttacgcaga tattcgctgg ctacttctgc cagtttgccg aacagcacaa cgcggtgcca
     7801 ttcagtctgc tctttcatct cgccggtcgc tttatcacgc caggattcgg aagtagccag
     7861 cgtaatgttg gcaacagcgc caccatttgg catgtagcgt acttccgggt cctgacccag
     7921 attaccaacg agaataacct tgtttacgcc tctgctggcc atgttcgtgt ctcctgaaaa
     7981 aaaatcgttc tgaataagtg taaacgcgcg attgtaccat taccaatagc gcttttacta
     8041 tgttgtgacc tcggttccgg gaaacaaacc tggccagaca ttgttacaca acactccggg
     8101 taatgcattc caatactgta tattcattca ggtcaatttg tgtcataatt aaccgtttgt
     8161 gatcgccggt agcaccatgc caccgggcaa aaaagcgttt aatccgggaa aggtgaatgg
     8221 ataagatcga agttcggggc gcccgcaccc ataatctcaa aaacatcaac ctcgttatcc
     8281 cccgcgacaa gctcattgtc gtgaccgggc tttcgggttc tggcaaatcc tcgctcgctt
     8341 tcgacacctt atatgccgaa gggcagcgcc gttacgttga atccctttcc gcctacgcgc
     8401 ggcagtttct gtcactgatg gaaaagccgg acgtcgatca tattgagggg ctttctcctg
     8461 ccatctcaat tgagcagaaa tcgacgtctc ataacccgcg ttctacggtg gggacaatca
     8521 ccgaaatcca cgactatttg cgtttgttgt tcgcccgcgt cggcgaaccg cgctgtccgg
     8581 accacgacgt cccgctggcg gcgcaaaccg tcagccagat ggtggataac gtgctgtcgc
     8641 agccggaagg caagcgtctg atgctgctcg cgccaatcat taaagagcgc aaaggcgaac
     8701 acaccaaaac gctggagaac ctggcaagcc agggttacat ccgtgctcgt attgatggcg
     8761 aagtctgcga tctttccgat ccgccgaaac tggaactgca aaagaaacac actattgaag
     8821 tggtggttga tcgcttcaag gtgcgtgacg atcttaccca acgtcttgcc gagtcgtttg
     8881 aaaccgcgct ggagctttcc ggtggtaccg cagtagtggc ggatatggac gacccgaaag
     8941 cggaagagct gctgttttcc gccaacttcg cctgcccaat ttgcggctac agtatgcgtg
     9001 aactggagcc gcgactgttt tcgtttaaca acccggcggg ggcctgcccg acctgtgacg
     9061 gccttggcgt acagcaatat ttcgatcctg accgcgtgat ccagaatccg gaactgtcgc
     9121 tggcaggtgg tgcgatccgt ggctgggatc gccgcaactt ctattatttt cagatgctga
     9181 aatcgctggc agatcactat aagttcgacg tcgaagcgcc gtggggaagc ctgagcgcga
     9241 acgtgcataa agtggtgttg tacggttctg gcaaagaaaa cattgaattc aaatacatga
     9301 acgatcgtgg cgatacctcc attcgtcgtc atccgtttga aggcgtgctg cacaatatgg
     9361 agcgccgtta taaagagacg gagtccagcg cggtacgcga agaattagcc aagtttatca
     9421 gtaatcgtcc gtgtgccagc tgcgaaggga cgcgtctgcg tcgggaagcg cgccacgtgt
     9481 atgtcgagaa tacgccgctg cccgccatct ccgacatgag cattggtcat gcgatggaat
     9541 tcttcaacaa tctcaaactc gcaggtcagc gggcgaagat tgcggaaaaa atccttaaag
     9601 agattggtga tcgcctgaaa ttcctcgtta acgtcggtct gaattacctg acactttccc
     9661 gctcggcaga aacgctttct ggcggtgaag cacagcgtat ccgtctggcg agccagattg
     9721 gtgcgggcct ggttggcgtt atgtatgtac tggacgagcc gtctatcggc ctgcaccagc
     9781 gcgataacga gcgcctgttg ggtacactta tccatctgcg cgatctcggt aataccgtga
     9841 ttgtggtgga gcacgacgaa gacgcgattc gcgccgctga tcatgtgatc gatatcggtc
     9901 ctggtgcggg tgttcacggc ggtgaagtgg tcgcagaagg tccgctggaa gcgattatgg
     9961 cggtgcctga atcgttgacc gggcagtaca tgagcggtaa acgcaagatt gaagtaccga
    10021 agaaacgcgt tccggcgaat ccagaaaaag tgctgaagct gacaggcgca cgcggcaaca
    10081 acctgaaaga cgtgacgctg acgctgccag tcggtctgtt tacctgcatc acaggggttt
    10141 caggttccgg taaatcaaca ctgattaacg acacactgtt cccgattgcc caacgccagt
    10201 tgaatggtgc gaccatcgcc gaaccggcac cctatcgcga tattcaggga ctggagcatt
    10261 tcgataaagt gatcgatatc gaccaaagcc caattggtcg tactccgcgt tctaacccgg
    10321 cgacctatac cggcgtgttt acacctgtgc gcgaactgtt tgcgggcgta ccggaatccc
    10381 gtgctcgtgg ttatacgcca ggacgtttca gctttaacgt ccgtggcgga cgctgcgagg
    10441 cctgtcaggg cgatggcgtg atcaaagtgg agatgcactt cctgccggac atttacgtgc
    10501 cgtgcgacca gtgcaaaggt aaacgctata accgtgaaac gctggagatt aagtacaaag
    10561 gcaaaaccat ccacgaagtg ctggatatga ccatcgaaga ggcgcgtgag ttctttgatg
    10621 ccgtacctgc actggcgcgt aagctgcaaa cgttgatgga cgttggcctg acgtacattc
    10681 gactggggca gtccgcaacc accctttcag gcggtgaagc ccagcgcgtg aagctggcgc
    10741 gtgagctgtc aaaacgcggc accgggcaga cactgtatat tctcgacgag ccgaccaccg
    10801 gtttgcactt cgccgatatt cagcaactgc tcgacgtgct gcataaactg cgcgatcagg
    10861 gcaataccat tgtggtaatt gagcacaatc tcgacgtgat taaaaccgct gactggattg
    10921 tcgacctggg accggaaggc ggcagtggcg gcggcgagat cctcgtctcc ggtacgccag
    10981 aaaccgtcgc ggagtgcgaa gcttctcata cggcgcgctt cctcaagccg atgctgtaat
    11041 cgtgaaggcc gctttctgag cggccttttc ctttcagagt tgcaccagca atttacgttt
    11101 ttcttccggc agtaaattca ccgcctgctg ataagacgca tccaccagat aatagatttg
    11161 cgaatcgggc agcgaaccgt cgagatacac ggtgctccag tgcgctttat tcagatggcg
    11221 gcttggacgc acatcgctgt gctgctgacg tagcagctcc gccagctccg ggctggtttt
    11281 cagcgaaaca gctgggcgat tttctacttc tttcaccatc gcaaacagta catcttccac
    11341 tttgatctgc gtcgctttcc agtcgttatg cacgctctgt tctgcgcctg gttttgccat
    11401 gcaatattgt agcaactccg aaatggtcat tttttactcc ccttgtagtg tcgcgatgat
    11461 gcgacgcgat ccgccgtgga tgcgatgttc ccccagccaa atgccttgcc aggtgccggt
    11521 ctgaatacgc cctttatgca ccggcaatac aagcgatgtt cccagcattg aggatttgat
    11581 atgagaaggc atatcgtctg ctccctcata gtcatgctca tagtttccat tatcgggaac
    11641 ggtgcggagg aaaaaacgct ccatgtcgtg gcgtacggtg ggatcgcagt tctcattaag
    11701 tgtcagagag gcggaggtat gttgcagcaa cagatgcagt aagccgatgt taacgcgcgg
    11761 catatcagcc agctgattca gaatttcatc cgttaccaga tgaaacccac gagatttggc
    11821 gctaagcgtg agcgtctttt gataccacat gtgctgctcc ttgataaaac tctcttaatc
    11881 agtttgcagc aagacagcga aaggataaag gtgtgattaa aaaaacagca ttcgggagag
    11941 catcacgctc tcccgctctg tcagtattct gaattgacga tcacctcttc accaaacgca
    12001 cccgcttgtg gcaagggttt gtaggtagag ttggaggcgc gcagaatgcg gataccacga
    12061 gcgccgacat cgcgtgcggc ggtaatatcg ttatcggaat cgccataaaa aattcggata
    12121 tttttatcct gcagccattg cgattttgta ttttgccctg gtttatcgcc cgcaaagatc
    12181 accggattca tgctggtggc aggaatatga aaattatccg ccagcgtttt tgaaaccgtt
    12241 tctgttttcg tcgggctacg accagtcaca aagaagatcg cgtcaccacg gcgtacatgc
    12301 atatcaatca gctggcgagc gacctctttt ggaatgctga attcatccca gccattgttc
    12361 attttttccc agaacacagg atttttcaga taatcttcgc tttctggcga gaaggttttt
    12421 ttgccgcgcc agaagcccgg actggaaaaa agtaccgtgt catcgatatc aaaccccacc
    12481 gccattggcg gacgccctgc gaggctattt tcaatttgtg cgaccgaaac ccaatgaatg
    12541 ggtgcctgtt cagcaagcct ggcaacgtta gtaccagggt taagcggtga aggagatgag
    12601 gccagggcaa cagcggaact gtttagagcg aacaataagc aaacggcact gattgcctgt
    12661 gtgatcttgc gcatattttt ccctaaatag tcagtatgtt gaaacttttt attgtgagat
    12721 ttgttgcaaa aaccatctga ccataacgcc agcagcaggg gaaaggaagg attatctgcg
    12781 gtttttgtga tgaaaagcag gatgattata tttaacccag taaatacgtt
//
LOCUS       DAPGOF010000138        12799 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.139, whole genome shotgun sequence.
ACCESSION   DAPGOF010000138 DAPGOF010000000
VERSION     DAPGOF010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.139"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            125..916
                     /gene="nanR"
                     /locus_tag="RHV13_003505"
     CDS             125..916
                     /gene="nanR"
                     /locus_tag="RHV13_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HDV1243007.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            1038..1931
                     /gene="nanA"
                     /locus_tag="RHV13_003506"
     CDS             1038..1931
                     /gene="nanA"
                     /locus_tag="RHV13_003506"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDV1243008.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            2040..3530
                     /gene="nanT"
                     /locus_tag="RHV13_003507"
     CDS             2040..3530
                     /gene="nanT"
                     /locus_tag="RHV13_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HDV1243009.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            3578..4267
                     /locus_tag="RHV13_003508"
     CDS             3578..4267
                     /locus_tag="RHV13_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDV1243010.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPDIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            4264..5139
                     /gene="nanK"
                     /locus_tag="RHV13_003509"
     CDS             4264..5139
                     /gene="nanK"
                     /locus_tag="RHV13_003509"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HDV1243011.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHLSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQETAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            5136..5600
                     /locus_tag="RHV13_003510"
     CDS             5136..5600
                     /locus_tag="RHV13_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDV1243012.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(5676..5981)
                     /locus_tag="RHV13_003511"
     CDS             complement(5676..5981)
                     /locus_tag="RHV13_003511"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV1243013.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVMDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(5998..6477)
                     /locus_tag="RHV13_003512"
     CDS             complement(5998..6477)
                     /locus_tag="RHV13_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HDV1243014.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCR"
     gene            complement(6661..8079)
                     /gene="gltD"
                     /locus_tag="RHV13_003513"
     CDS             complement(6661..8079)
                     /gene="gltD"
                     /locus_tag="RHV13_003513"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HDV1243015.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVQAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TNDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHGMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            complement(8092..12552)
                     /gene="gltB"
                     /locus_tag="RHV13_003514"
     CDS             complement(8092..12552)
                     /gene="gltB"
                     /locus_tag="RHV13_003514"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HDV1243016.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
BASE COUNT         2791 a         3449 c         3490 g         3069 t
ORIGIN      
        1 atagttaatt attccgttaa ttatggtaat gatgaggcac aaagagaaaa ccctgccatt
       61 ttcccctact ttcaatcctg tgataggatg tcactgatga tgttaatcac actgacctta
      121 cagaatgggc cttatgaacg catttgattc gcaaaccgaa gattcttcac ctgcaattgg
      181 tcgcaacttg cgtagccgcc cgctggcgcg taaaaaactc tccgaaatgg tggaagaaga
      241 gctggaacag atgatccgcc gtcgtgaatt tggcgaaggt gaacaattac cgtctgaacg
      301 cgaactgatg gcgttcttta acgtcgggcg tccttctgtg cgtgaagcgc tggcagcgtt
      361 aaaacgcaaa ggtctggtgc aaataaacaa cggcgaacgc gctcgcgtct cgcgtccttc
      421 tgcggacacc atcatcggtg agctttccgg catggcgaaa gatttccttt ctcatcctgg
      481 tgggattgcc catttcgaac aattacgtct gttctttgaa tccagcctgg tgcgctatgc
      541 ggctgaacac gccaccgatg agcaaatcga tttgctggca aaagcactgg aaatcaacag
      601 tcagtcgctg gataacaacg cggcattcat tcgttcagac gttgatttcc accgcgtgct
      661 ggcggagatc cccggtaacc caatcttcat ggcgatccac gttgccctgc tcgactggct
      721 tattgccgca cgcccaacgg ttaccgatca ggcactgcac gaacataaca acgttagtta
      781 tcaacagcat attgcgatcg ttgacgcgat ccgccgccac gatcctgacg aagccgatcg
      841 tgcgttgcaa tcgcatctca acagcgtctc tgctacctgg cacgccttcg gtcagaccac
      901 caacaaaaag aaataatgcc actttagtga agcagatcgc attataagct ttctgtatgg
      961 ggtgttgctt aattgatctg gtataacagg tataaaggta tatcgtttat cagacaagca
     1021 tcacttcaga ggtatttatg gcaacgaatt tacgtggcgt aatggctgca ctcctgactc
     1081 cttttgacca acaacaagca ctggataaag cgagtctgcg tcgcctggtt cagttcaata
     1141 ttcagcaggg cattgacggt ttatacgtgg gtggttcgac cggcgaagcc tttgtacaaa
     1201 gcctttccga gcgtgaacaa gtactggaaa tcgtcgccga agaggcaaaa ggtaagatta
     1261 aactcatcgc ccacgtcggt tgcgtcagca ccgccgaaag ccaacaactt gcggcatcgg
     1321 ctaaacgcta tggcttcgat gccgtctccg ccgtcacgcc gttctactat cctttcagct
     1381 ttgaagaaca ctgcgatcac tatcgggcaa ttattgattc ggcggatggt ttgccgatgg
     1441 tggtgtacaa cattccagcc ctgagcgggg taaaactgac cctggatcag atcaacacac
     1501 ttgttacatt gcctggtgtc ggtgcgctga aacagacctc tggcgatctc tatcagatgg
     1561 agcagatccg tcgtgaacat cctgatctgg tgctctataa cggttacgac gaaatcttcg
     1621 cctctggtct gctggcgggc gctgatggtg gtatcggtag tacctacaac atcatgggct
     1681 ggcgctatca ggggatcgtt aaggcgctga aagaaggcga tatccagacc gcgcagaaac
     1741 tgcaaactga atgcaacaaa gtcattgatt tactgatcaa aactggtgta ttccgcggcc
     1801 tgaaaactgt tctgcattat atggatgtcg tttctgtgcc gctgtgccgc aaaccgtttg
     1861 gtccggtaga tgaaaaatat ctgccagaac tgaaggcgct ggcccagcag ttgatgcaag
     1921 agcgcgggtg agttgtttcc cctcgctcgc ccctaccggg tgaggggaaa taaacgcatc
     1981 tgtaccctac aattttcata ccaaagcgtg tgggcatcgc ccaccgcggg agactcacaa
     2041 tgagtactac aacccagaat atcccgtggt atcgccatct caatcgtgca caatggcggg
     2101 cattttccgc tgcctggttg ggatatctgc ttgacggttt tgatttcgtt ttaatcgccc
     2161 tggtactcac cgaagtacaa ggtgaattcg ggctgacgac ggtgcaggcg gcaagtctga
     2221 tctccgcagc ctttatctct cgctggttcg gcggcctgat gctcggcgct atgggtgacc
     2281 gctacgggcg tcgtctggca atggtcacca gcatcgttct cttctcggcc gggacgctgg
     2341 cctgcggctt tgcgccaggc tacatcacca tgtttatcgc tcgtctggtc atcggcatgg
     2401 ggatggcggg tgaatacggt tccagcgcca cctatgtcat tgaaagctgg ccaaaacatc
     2461 tgcgtaacaa agccagtggc tttctgattt caggcttctc tgtaggggcc gtcgttgccg
     2521 ctcaggtcta cagcctggtg gttccggtct ggggctggcg tgcgctgttc tttatcggca
     2581 ttttgccaat catctttgct ctctggctgc gtaaaaacat cccggaagcg gaagactgga
     2641 aagagaaaca tgcaggtaaa gcaccggtgc gcacgatggt ggatattctc taccgtggtg
     2701 aacatcgcat cgccaatatc gtgatgacgc tggcggcggc tactgcgctg tggttctgct
     2761 tcgccggtaa cctgcaaaat gccgcaatcg tcgctgttct cgggctgtta tgcgctgcaa
     2821 tctttatcag ctttatggtg cagagtacgg gcaaacgctg gccaactggc gtaatgctga
     2881 tggtggtcgt gttgtttgct ttcctctact catggccgat tcaggcgctg ctgccaacgt
     2941 atctgaaaac cgatctggct tataacccgc atactgtcgc caatgtgctg ttctttagtg
     3001 gctttggcgc ggcggtgggg tgctgcgtcg gtggcttcct cggtgactgg ctgggaaccc
     3061 gcaaagcgta cgtttgtagt ctgttggcct cgcagctgct gattattccg gtatttgcta
     3121 ttggcggtgc aaacgtctgg gtgctcggtc tgttactgtt cttccagcaa atgcttggac
     3181 aagggatcgc cgggatctta ccgaaactga ttggcggtta tttcgatacc gaccagcgtg
     3241 cagcgggcct gggctttacc tataacgttg gtgcattggg tggcgcactg gccccaatca
     3301 tcggcgcgtt gatcgctcaa cgtctggatc tgggtactgc gctggcatcg ctctcgttca
     3361 gtctgacatt tgtggtgatc ctgctgattg gtctggatat gccttctcgt gttcagcgtt
     3421 ggctgcgccc ggaagcgttg cgtactcatg acgctatcga cggcaaacca tttagcggtg
     3481 ccgtgccgtt tggcagcgcc aaaaacgatt tagtcaaaac caaaagttaa tcctgttgcc
     3541 cggtctctgt accgggcctt tcgctaaggg aagatgtatg tcgttacttg cacaactgga
     3601 tcaaaaaatc gctgctaacg gtggcctgat tgtctcctgc cagccggttc cggacagccc
     3661 gctcgataaa cccgacattg tcgctgccat ggcattagcg gcagagcagg cgggcgcggt
     3721 tgccattcgc atcgaaggtg tggcaaatct gcaagccacg cgtgcggtgg tgagcgtgcc
     3781 gattatcggg attgtgaaac gcgatctgga ggattctccg gtacgcatca cggcctatat
     3841 tgaagatgtc gatgcgctgg cgcaggcggg cgcggacatt atcgccattg acggcaccga
     3901 ccgcccgcgt ccggtgcctg ttgaaacgct gctggcacgt attcatcatc acggtttact
     3961 ggcgatgacc gactgctcaa cgccggaaga cggcctggca tgccaaaagc tgggagccga
     4021 aattattggc actacgcttt ctggctatac cacgcctgaa acgccagaag agccggatct
     4081 ggcgctggtg aaaacgttga gcgacgccgg atgtcgggtg attgccgaag ggcgttacaa
     4141 cacgcctgct caggcggcag acgcaatgcg ccacggcgcg tgggcggtga cggtcggttc
     4201 tgccattacg cgtcttgaac acatttgtca gtggtacaac acagcgatga aaaaggcggt
     4261 gctatgacca cactggcgat tgatatcggc ggtactaaac ttgccgccgc gctgattggc
     4321 gctgacgggc agatccgcga tcgtcgtgaa cttcctacgc cagccagcca gacaccagaa
     4381 gccttgcgtg atgccttatc cgcattagtc tctccgttgc aagctcatgc gcagcgggtt
     4441 gccatcgctt caaccggaat tattcgcgac ggcagtttgc tagcgctgaa tccgcataat
     4501 ctcggtggat tgctacactt tccgttagtc aaaacgctgg aacaacttac caatttgccg
     4561 accattgcca ttaacgacgc gcaggccgca gcatgggcag agtatcaggc gctggaaggc
     4621 gatataaccg atatggtctt tatcaccgtt tccactggcg tcggcggcgg tgtagtgagc
     4681 ggcggcaaac tgcttaccgg ccctggcggt ctggcggggc acatcgggca tacgcttgcc
     4741 gatccacacg gcccagtctg cggctgtgga cgcacaggtt gcgtggaagc aattgcttct
     4801 ggtcgcggca ttgcggcggc agcgcagggc gagctggctg gcgcggatgc tagaactatt
     4861 ttcacacgcg ccgggcaggg cgatgagcag gcgcagcaac tgattcatct ctccgcacgt
     4921 acgcttgcaa ggctgatcgc tgatattaaa gccacaactg attgccagtg cgtggtggtt
     4981 ggtggcagcg ttggtctggc agaagggtat ctggcgttgg tggaaatgta tctggcgcag
     5041 gagacagcgg catttcatgt tgatttactg gcggcgcatt accgccatga tgcaggttta
     5101 cttggggctg cgctgttggc ccagggagaa aaattatgat gatgggtgaa gtacagtcat
     5161 taccgtctgc tgggttacat cctgcgttac aggacgcgtt aacgctggcc ttagctgcca
     5221 gaccgcaaga aaaagcgccg ggtcgttacg aattacaggg cgacaatatt tttatgaatg
     5281 tcatgacgtt taacactcaa tcgcccgtcg agaaaaaagc ggaattgcac gagcaataca
     5341 ttgatatcca gctgttatta aacggtgagg aacggattct gtttggcatg gcaggcactg
     5401 cgcgccaatg tgaagagttc caccatgaag atgattacca gctttgtagc gccattgaaa
     5461 acgagcaaac catcatcctg aaaccgggaa tgttcgccgt gtttatgcca ggagagccgc
     5521 ataagccggg atgcgttgtt ggcgagccgg gtgagattaa aaaggtggtg gtgaaggtta
     5581 aggctgattt aatggcttaa atttattaga tcaggtgtga cctgacccat ctttggcaca
     5641 gagccaaacc tgaactgacg gaccgctctg tgccattacc tgacgttgta ttcttttatg
     5701 ttaacaccct tgatactgta tatacctgtc cgcttaattc gcttgccctt tttattcgta
     5761 atagttgctg taagtgtact gcagcgaatg gaatgacgtt catccatcac cagccgaatt
     5821 gcttctgaag catcttcaac ctctagaagg tcgtgagtct tatttaagcc gaatggaaca
     5881 aggtggaagg cagtttttcc tgatgatttt ctggttattt ttactggata caacacatca
     5941 ccacttttat ggattaattc aagctgttca atgtaaatca tggcaacttt ccttcgttta
     6001 tctgcatgaa tatacaaacc gaaaatagga gatgcaatac aaaagctgtg gaaatatcaa
     6061 acagcctctc aatttggcag acagcgtctg ccaaatcttt gccgatggga attctgcaga
     6121 tggtgtctgc ggaattccca atggtgttaa ttcgccagac actgtctggc gaatttcaac
     6181 atgttgaaaa aagaggtaaa aaagcctcat ttggcgtaag ttttttgcag agaaaccacg
     6241 cttatacctt agacttaaat cctttgatag tcgcttgatt agctgcgcac catacgcagc
     6301 cctggcctcg ccaccttgtt caaattcgac aattcgtcgg ccaatttccc agtatgtcgc
     6361 ggtcatcagc gcgttaacgc tacgtaccgt ttccgtccgg gcgctatcga ccaggtgaat
     6421 aataccgtca tggatttgct ggtagcctgt tgaggttctc tctgagagcg attccattat
     6481 ctgtcccttt atatggcgct gcgttgaata atggaatcag ctatacgccg ggcatctacc
     6541 ggtaacaatc cagatgagcg tattacattg gttttcttca gattatgcga ggcgctttcc
     6601 agaaagaaag gagaatttga gcgccgactc ttttctgagg aggcggcgct gggacagagc
     6661 ttaaacttcc agccagttca taataccgtc tgccgcctta cgaccttcgg caatagcggt
     6721 taccaccaga tcggaaccac ggacgatatc gccgccagca aagattttcg ggttgctggt
     6781 ctggaaagca ttgtcgctgc cttccggagc gatgatgcgg ccttgcgaat ccagttcgac
     6841 gctgtgtttc gccagccatt ccatgccgtg tgggcgaaaa ccaaacgcca tgatcaccgc
     6901 atctgccgga acgatatgtt cggaacctgc cacaatctcc gcgcggcgac ggcctttggc
     6961 atccggttcg cccatttcgg tacgcaccat tttcacaccg ctgactttgc cgttgccatt
     7021 tacttctata cccagcggct ggacgttgaa tttgaactct acgccttctt cccgcgcgtt
     7081 tttcacttcg cggcgggaac ccggcatgtt ctcttcatca cgacgatagg cacaggttac
     7141 gtgcttcgct ccctggcgta cagacgtgcg cacgcagtcc atcgcagtgt cgccaccgcc
     7201 aagtactacc acgcgtttac cttccatgct gacgaacggt tcgtcgttgg tttcaccaaa
     7261 gcccattaac tgtttggtgt tggcgatgag gaacggcagc gctgcgtaca cgccatcagc
     7321 gtcttcgttt tccagcccgc cgcgcattga ctgataagtc ccgacgccaa ggaacacggc
     7381 atcgtaatca ctcagcagat cgtccagctg cacgtcgcgg cccacttcgg tattgagttt
     7441 gaattcaata cccatgccgg tgaagatttc acggcggcgc gtcattacct ctttttccag
     7501 tttgaaagcc ggaataccga aggtcagcaa gccgccaatt tccgggtgac ggtcgaagac
     7561 gaccgcttgt acgccgttgc gcgtcaggac atctgcacac gccagacctg ccggacctgc
     7621 gccgataatc gccacttttt taccggtctg tttcacgccg gacatatccg gacgccagcc
     7681 catttcgaac gctttatcat tgatataacg ctcaatgttg ccgatggtca ccgcgccaaa
     7741 ctcatcgttc agggtgcagg aaccttcgca cagacggtct tgcgggcaga cacgtccgca
     7801 tacttccggc agagtgttgg tctgatgcga cagttctgcc gcttcaaaaa tacgcccctc
     7861 gttggcgagc ttaagccagt tcgggatgta gttgtgtacc gggcatttcc actcgcagta
     7921 tgggttgccg cacgacaggc agcggtcagc ctgcgctttg gcctggcctt cggaaaacgg
     7981 ctcgtaaatt tcaacaaact caattttgcg gatcttcagc ggtttctttg gcggatcaac
     8041 gcgctgcagg tcgataaatt gataaacatt ctgactcatt gttgctaccc cttactgcgc
     8101 ctgcacgcgc aactctgctg cgctacgact acgatgaccc agcagtgctt tgacatcact
     8161 ggacttcggt ttaaccagcg caaatttagt ggcgaaggtt gaccagttcg ccagaatctc
     8221 ttcaccacgc tgagaaccgg tatgctgaac atgctcggtg ataagaccac gcagatgctc
     8281 ttcatggatc gccagatcgt caacgcttaa gacctcgacc agttccgggt taacgcgttt
     8341 gcggaaatcg ccgctttcat cgagaacgta agcgaagccg ccggtcatgc ccgcaccgaa
     8401 gttaacgccg gttttaccca gaatgcagac gataccgccc gtcatatatt cacaaccgtt
     8461 gtcgccaatg ccttctacca cggtgatagc accggagtta cgcacgccga aacgttcacc
     8521 cgcgcggcct gcggcataca gacgaccacc ggtcgcgcca tacaggcagg tgttgccgat
     8581 aatacttgct tcatggctgc ggaaggcgga accaaccgga ggacgaatgg cgattaagcc
     8641 gcccgccatg cctttaccga catagtcgtt ggcatcaccg gtcaggtaca gctccacgcc
     8701 gcccgcgttc cacacgccga agctctggcc tgcagtgccg ttgaagtacg ctttaatcgg
     8761 atcggctgcc agcccctgat cgccatgcgt ctgggcgata tagcctgaaa gcgacgcgcc
     8821 gacagaacgg tcggtgttgc ggatatcgaa ccagaaagtt ttgctctggc gttcatcgac
     8881 aaacggtttc gcctgttgca gcaactgcgc gttcagcagg ccgttatcaa acggcggatt
     8941 gttttcggta cagtagagcg ccttacctgc atgcggctcg gcagtctcaa gcagcttcga
     9001 cagcgccagt ttctgctgtt tggcggtgaa accgtccagc tctttcagca ggtcggtgcg
     9061 accaatcaga tccaccagac gtgttacgcc aagctgtgcc atcagctcgc gtgtttcacg
     9121 ggcgataaat tcaaagtaat tcgtcacctt gaatggcagg ccgtgatagt gattcttacg
     9181 cagtttgtca tcctgagttg ctacacccgt ggcgcagttg ttcaggtggc aaatacgcag
     9241 atatttacaa ccgagcgcca ccattgggcc agtgccgaag ccgaagcttt ccgcgccgag
     9301 aatcgccgct ttgatgatat caacgccggt tttcaggccg ccatcaacct gcaaacggat
     9361 tttatgacgc aagccgttgg caaccagcgc ctgttgtgtt tcaacaagtc ccagctccca
     9421 cggacagcct gcgtatttca ccgatgaaag cggacttgcg ccggtgccgc cgtcatagcc
     9481 tgcgatggtg attaaatcgg cataagcttt tgccacgcca gtcgcgatgg tgccaacgcc
     9541 cggttcggaa accagcttca cggagatcat cgctttcggg ttaacctgct tgaggtcgaa
     9601 aatgagctgc gctaagtctt cgatagagta gatatcgtgg tgcggcggcg gggagatcag
     9661 cgtcacgccc ggcaccgaat agcgcagttt ggcaatgtaa ggagtgactt tgtcgcccgg
     9721 caactgaccg ccttcgcccg gcttcgcgcc ctgggcgact ttaatctgaa tgacgtcggc
     9781 attgaccaga tacgccggag taaccccaaa gcgaccggaa gccacctgct tgatgcgcga
     9841 cactttgttg gtgccgtagc gcgccggatc ttcaccgcct tcaccggagt tcgagtttcc
     9901 gccgatgctg ttcatcgctt ccgccagcgc ctcgtgggct tccgggctta acgcgccgat
     9961 agacatcgca gcggtatcga agcgtttaaa cagttcgctt gccggttcaa catcagcaat
    10021 gttgaccgcg ttttcacccg gcgtaattgc cagcagatcg cgcagcgtgg ttgccggacg
    10081 ctcattaacc agcttcgcgt attcctgata gtcgctgtac tcgccgcttt gtaccgcctg
    10141 ttgcagcgtg cgcaccacgt ccgggttgta ggcgtggtat tcgccgccgt ggacgtattt
    10201 cagcaggccg ccctggctga tgggcttacg cgccagccag gcacgtttcg agagattcag
    10261 cagatcctgc tggaagtctt caaagcttgc cccaccaatg cgactgaccg ccccctggaa
    10321 gcacaggccc actacatcat cgtgcagacc gaccgcttca aacagtttcg agcagcggta
    10381 agaggcgatg gtggaaatgc ccattttgga catgattttg tacaagcctt tgttgatgcc
    10441 gttacggtag ttgagcatca cggtacgata atctttggca atcgcgtggg tgtctaccag
    10501 gcggcccagc gtttcatagg caaggtacgg ataaatagcc gtcgcaccga agcccagcaa
    10561 cacggcgaaa tggtgcggat cgcgggcgct ggcggtttcg acgatgatgt tggcatcgca
    10621 acgcaggctt tgatcgacca gacgggtctg gatcgcgcca accgccatcg gagccggaac
    10681 cggcaggcga tctttagcga tattccggtc ggagagcacc agcagcacgg tgccgctacg
    10741 taccattttt tcggctttgt cgcacagctc tttgaccgtc gcttcgagcg tggttttagt
    10801 gacgtcaaag gtgatatcca gcgtatctgc gcggtagtgc tcctctttca tcgtcgtgag
    10861 ctgtttgaaa tccgagtaga gcagaatcgg cgatttaaag cttaaacggt gcgcctggcc
    10921 ctctgcttca cagaagacgt tcatttcacg accaatgctg gtggcgagcg acataacatg
    10981 cgcttcacgc agcgggtcga ttggcgggtt agtcacctgg gcaaactgct ggcggaagta
    11041 gtcgtaaata atgcgcggct gactggagag cacggcgaat ggggtgtcgt cgcccatcga
    11101 accgaccgct tcctgaccgt tttcgcccag tacgcgaatt acggagtcca gctcttccgc
    11161 gctgtagtta aactgtttct ggtagctggc aagtgtgtcg tcgtccagtt cgcggctacc
    11221 cacttcttca tcgggcagat cttcaaacgg tacaagtcgg cggacgtttt tctccatcca
    11281 ctctttatat ggatggcggc ttttcagatc gtcatcggtt tctgccgagt gcagaatacg
    11341 cccactgcgg gtgtcgataa ccatcagttc gcctggcccg acgcggcctt tttcgaccac
    11401 ttcgtcaggc tggtaatccc agataccgac ttcagaggcg caggtgatga gcttatcttt
    11461 ggtaatgacg tagcgtgccg gacgcagacc gttacggtcg aggttacagg cagcgaagcg
    11521 accgtcggac atcacgatgc ccgccggacc atcccacggc tccatatgca tggagttaaa
    11581 gtcaaagaag gcacgcagtt ccgggtccat atccggattg ttctgccagg cgggtggtac
    11641 taacaggcgc atggcgcgga tgatatccat cccgcctgcc agcagcagtt ccagcatgtt
    11701 atccatggaa ctggagtcag agccggtttc gttgacgaac ggtgcggcgt cgtgcaggtc
    11761 agggataagc ggtgtctgga atttataagt acgcgcacgc gcccattggc ggttaccggt
    11821 gatggtgttg atttcaccgt tatgcgccag atagcggaac ggttgcgcca gcggccagcg
    11881 cggtacggtg ttagtggaga agcgctggtg gaacaggcaa atggccgatt ccagacgcag
    11941 gtccgcaaga tccagataaa agcgcggcag atccgccggc atacacagac ctttatagat
    12001 gttcaccaga ttcgacaggc tacagacgta gaagtctttg tcggcttcga gacgcttttc
    12061 aatgcggcgg cgggcgataa acagacggcg ctccatatcg cgcggacgcc agcctgccgg
    12121 ggcgttcaca aaaatttgct caatgcgtgg cagagaggag agggcgattt cacccaacac
    12181 gccttcgtta gtggggacat cacgccagcc cacaatcgac aaggtttcgc gttgcagttc
    12241 ttcttcaacg atgcggcgtg cggcagcggc gagttcagga tctttattca ggaatagcat
    12301 cccgacggcg tagtttttcg ctaaacgcca gccgcgctcc tgcgcaacga tgcgaaaaaa
    12361 gcgatccggt ttttgtaaca gcaagccgca accgtcaccg gttttaccat cggcgagaat
    12421 cgcgccacgg tgctgcatgc gggccagtgc gtgtattgca gtacgcacta ccttgtggct
    12481 aggttcgcct tctatgtggg cgatcaggcc gaaaccacag ttatccctct caagggattt
    12541 atcgtacaac atatcgtgaa cctcccccag gctctgcggg aaccccactt ccgaaccgct
    12601 gcgcacgggc acagaaagag cgtggcgacg gggtttgcgt gtcatacgcg cgcctcgcat
    12661 tcgccccctt gttgtccttt tcgcatcggt taatacggtg aagaggggat ctcaattact
    12721 gcataaatat gatgagcagg ctgctcatcc agaaagcttc cagcggattt ccaacttatc
    12781 gggaaagctg atacaggtc
//
LOCUS       DAPGOF010000139        12779 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.140, whole genome shotgun sequence.
ACCESSION   DAPGOF010000139 DAPGOF010000000
VERSION     DAPGOF010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.140"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            23..544
                     /gene="fecI"
                     /locus_tag="RHV13_003515"
     CDS             23..544
                     /gene="fecI"
                     /locus_tag="RHV13_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HDV1243017.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            541..1494
                     /gene="fecR"
                     /locus_tag="RHV13_003516"
     CDS             541..1494
                     /gene="fecR"
                     /locus_tag="RHV13_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HDV1243018.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            1581..3905
                     /gene="fecA"
                     /locus_tag="RHV13_003517"
     CDS             1581..3905
                     /gene="fecA"
                     /locus_tag="RHV13_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HDV1243019.1"
                     /translation="MTPLRVFRKTTPLVNTIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLSPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYLNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            3950..4852
                     /gene="fecB"
                     /locus_tag="RHV13_003518"
     CDS             3950..4852
                     /gene="fecB"
                     /locus_tag="RHV13_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HDV1243020.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAAMVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            4849..5847
                     /gene="fecC"
                     /locus_tag="RHV13_003519"
     CDS             4849..5847
                     /gene="fecC"
                     /locus_tag="RHV13_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HDV1243021.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITQLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINTLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            5844..6800
                     /gene="fecD"
                     /locus_tag="RHV13_003520"
     CDS             5844..6800
                     /gene="fecD"
                     /locus_tag="RHV13_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HDV1243022.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            6801..7568
                     /gene="fecE"
                     /locus_tag="RHV13_003521"
     CDS             6801..7568
                     /gene="fecE"
                     /locus_tag="RHV13_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HDV1243023.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(8126..8383)
                     /locus_tag="RHV13_003522"
     CDS             complement(8126..8383)
                     /locus_tag="RHV13_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243024.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHKDIIDNNRSINWPV"
     gene            complement(8466..8738)
                     /locus_tag="RHV13_003523"
                     /pseudo
     CDS             complement(8466..8738)
                     /locus_tag="RHV13_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(8780..9993)
                     /locus_tag="RHV13_003524"
     CDS             complement(join(8780..9702,9702..9993))
                     /locus_tag="RHV13_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDV1243025.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQSEYDSQWATICSIAPKIGCTP
                     ETLRVWVRQHERDTGGGDGGLTTAERQRLKELERENRELRRSNDILRQASAYFGEGGV
                     RPPLEKMMPLLDKLREQYGVGPLCSELHIAPSTYYHCQQQRHHPDKRSARAQRDDWLK
                     KEIQRVYDENHKVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRTTIS
                     RKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWRGFVYVAFIIDVFAGYIVGWRVSSS
                     METTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYD
                     NAMAESINGLYKAEVIHRKSWKKRAEVELATLTWVDWYNNRRLLERLGHTPPAEAEKA
                     YYASIGNDDLAA"
     misc_feature    complement(9596..9712)
                     /locus_tag="RHV13_003524"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
     gene            10043..10222
                     /locus_tag="RHV13_003525"
                     /pseudo
     CDS             10043..10222
                     /locus_tag="RHV13_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327845.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10390..11460)
                     /gene="dadX"
                     /locus_tag="RHV13_003526"
     CDS             complement(10390..11460)
                     /gene="dadX"
                     /locus_tag="RHV13_003526"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HDV1243026.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(11470..12768)
                     /gene="dadA"
                     /locus_tag="RHV13_003527"
     CDS             complement(11470..12768)
                     /gene="dadA"
                     /locus_tag="RHV13_003527"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HDV1243027.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLISGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
BASE COUNT         2767 a         3721 c         3455 g         2836 t
ORIGIN      
        1 attaactttt gatgcactcc gcatgtctga ccgcgccact accacagctt ccttaacgtt
       61 cgagtcgctt tatggcacac atcacggctg gttgaaaagc tggctgacgc gcaaactcca
      121 gtctgctttt gatgcagatg acattgccca ggacactttt ttgcgggtaa tggtcagcga
      181 aacgctctcg acgatccgcg atcctcgctc cttcctctgc actatcgcca aacgcgtgat
      241 ggtggacctg tttcgccgaa acgcgctgga aaaagcgtat ctggagatgc tggcgcttat
      301 gccggagggg ggagctcctt cacctgagga acgcgaaagc caactcgaga ccctacaact
      361 cctcgacagc atgctggacg ggctaaacgg caaaacacgt gaagcgtttc tgctttcgca
      421 actggatggt ctgacataca gcgagattgc gcacaaactc ggtgtttcca tcagctccgt
      481 gaaaaaatac gtggcgaaag ccgtcgagca ctgcctgctg ttccgtctgg agtatgggtt
      541 atgaatcctt tgttaaccga ttcccgccgt caggcgctgc gttcagcttc ccactggtat
      601 gccgtgctaa gcggcgagcg cgtcagccca caacaggaag cgcgctggca acagtggtat
      661 gaacaggatc aggataacca gtgggcctgg cagcaggttg aaaacctgcg caaccagctt
      721 ggcggtgtgc ctggcgacgt tgccagcagg gcgttgcacg atacccgcct cacccgccgt
      781 cacgtgatga aaggattact gctgttgctc ggcgctggcg gaggctggca gctctggcag
      841 tcggaaaccg gcgaaggtct gcgggcagat taccgcaccg ccaaaggcac agtcagccgt
      901 cagcaactgg aagatggctc cctgctcacg ctgaataccc aaagcgcggc ggatgtgcgt
      961 tttgatgcgc atcagcgcac cgtccggctc tggtacggtg aaatcgccat tactaccgcg
     1021 aaagatgccc tgcaacgccc cttccgtgtc ctgacccgtc agggccagct cactgcttta
     1081 gggacagaat ttaccgtccg ccagcaggat aatttcacgc agcttgacgt gcagcagcac
     1141 gctgtggaag tgcttctcgc cagtgccccc gcgcaaaaac gcatcgtgaa cgctggtgaa
     1201 agcctgcagt tcagcgcctc tgagtttggc gcagtgaaac cgctggatga cgagagtaca
     1261 agctggacga aggacatcct gagcttcagc gataaaccgc tgggtgaggt gatagccacg
     1321 ctaacccgtt accgcaacgg cgtgctgcgc tgcgatcccg ccgttgccgg gctgcgcctg
     1381 agcgggacgt tcccgctgaa aaataccgat gcgatcctga acgttatcgc gcaaacgctt
     1441 cccgttaaaa ttcagtctat tacgcggtac tggataaaca tttcaccact gtaaggaaaa
     1501 taattcttat ttcgattgtc ctttttaccc ttctcgttcg actcatagct gaacacaaca
     1561 aaaatgatga tggggaaggt atgacgccgt tacgcgtttt tcgtaaaaca acacctttgg
     1621 ttaacaccat tcgcctgagc ctgctgccgc tggccggtct ctcgttttcc gcttttgctg
     1681 cacaggttaa tatcgcaccg ggatcgctcg ataaagcgct caatcagtat gccgcacaca
     1741 gcggatttac cctctcggtt gacgccagcc tgacgcgcgg caagcagagc aacggcctgc
     1801 acggcgatta cgacgtcgag agcggcctgc aacaactgct ggacggcagc ggactgcagg
     1861 taaaaccgct gggaaataac agctggacgc tggagcccgc gcccgcacca aaagaagatg
     1921 ccctgaccgt ggtcggcgac tggctgggtg atgcgcgtga aaacgacgta tttgaacatg
     1981 ctggcgcgcg tgacgtgatc cgccgtgagg atttcgccaa aaccggcgca accaccatgc
     2041 gtgaggtact taaccgcatc cctggcgtca gcgcgccgga aaacaacggc accggcagcc
     2101 acgacctggc gatgaacttt ggcatccggg gcctgagccc gcgcctcgcc agccgctcga
     2161 ccgtcctgat ggacggcatc cccgtcccct tcgcccctta cggtcagccg cagctttcac
     2221 tggctcccgt ttcgctcggc aacatggatg ccattgacgt ggtacgcggt ggtggtgcgg
     2281 tgcgttacgg accgcagagc gtgggcggcg tggtgaactt tgttacccgt gccattccgc
     2341 aggactttgg tatcgaggcg ggcgtggaag gtcagctcag cccaacctct tcacaaaaca
     2401 acccgaaaga gacgcacaac ctgatggtgg gcggcacagc ggacaacggt tttggcaccg
     2461 cgctgctcta ctccggcacg cgcggcagtg actggcgcga gcacagcgcc acccgcatcg
     2521 acgacctgat gctgaaaagc aaatatgcgc cggatgaggt gcacaccttc aacagcctgc
     2581 tgcaatatta cgatggtgaa gccgacatgc ccggtggcct gtctcgcgcg gattacgacg
     2641 ccgatcgctg gcaatccacc cgcccgtatg accgcttctg gggtcgtcgc aagctggcga
     2701 gcctgggcta ccagttccag ccagacagcc agcataaatt caacattcag gggttctaca
     2761 cccaaaccct gcgcagcggc tacctggagc aaggcaaacg catcaccctc tcgccgcgta
     2821 actactgggt gcgcggtatt gagccacgct acagccagat ctttatgatc ggcccttccg
     2881 cgcacgaagt gggcgtgggc tatcgctatt tgaatgaatc aacgcatgaa atgcgttact
     2941 acaccgccac cagcagcggg cagttgccgt ccggctcaag cccttacgac cgcgatacgc
     3001 gttccggcac cgaggcgcac gcctggtatc tggatgacaa aatcgacatc ggcaactgga
     3061 ccatcacgcc gggtatgcgt ttcgaacata tcgagtcata ccagaacaac gccatcacag
     3121 gcacgcacga agaagtgagc tataacgcac cgcttccggc gttgaacgtg ctctatcacc
     3181 tgactgacag ctggaatctt tatgcaaaca ctgaaggctc gttcggcacc gtacagtaca
     3241 gccagattgg caaggctgtg caaagcggca atgtggaacc ggaaaaagcg cgaacctggg
     3301 aactcggtac ccgctacgac gacggcgcgc tgacggcgga aatggggctg ttcctgatta
     3361 actttaacaa tcagtacgac tccaaccaga ccaacgacac cgtcactgca cgtggcaaaa
     3421 cgcgccatac cgggctggaa acgcaggcac gttacgatct gggtacgcta acgccaacgc
     3481 ttgataacgt ttccatctac gccagctatg cgtatgtgaa cgcggaaatc cgcgagaaag
     3541 gcgacaccta cggcaatctg gtaccattct ccccgaaaca taaaggcacg ctgggcgtgg
     3601 actacaagcc aggaaactgg acgttcaatc tgaacagcga tttccagtcc agccagtttg
     3661 cggataacgc caatacggtg aaagagagcg ccgacggcag taccggccgc attcccggct
     3721 tcatgctctg gggcgcacgc gtggcgtatg actttggccc gcagatggca gatctgaacc
     3781 tggcattcgg tgtgaaaaac atcttcgacc aggactactt catccgctct tatgacgaca
     3841 acaacaaagg catctatgca ggccagccgc gcacgctgta tatgcagggg tcgttgaagt
     3901 tctgatgtgt taacgcccgg cttgccgggc ttttagctgg aatgtgatta tgttggcatt
     3961 tatccgtttt ctttttgcag gcctgctgct ggtgatcagc cacgcctttg ccgccatggt
     4021 tcaggacgaa cacggcacgt ttacactcga aaaaacgcca caacggattg tggtgctgga
     4081 actctcgttc gccgatgcgc tggccgccgt ggacgtcagc ccgatcggta ttgccgacga
     4141 taacgatgca aaacgcatcc tgcccgaagt gcgtgcgcac ctgaaaccgt ggcagtccgt
     4201 cggaacgcgc gcgcagccga gcctggaagc cattgccgct ctgaaaccag acctgatcat
     4261 tgccgacagc agtcgccatg cgggggttta catcgccttg cagcaaatcg cgccggtact
     4321 gctgcttaag tcccgcaacg aaacctacgc tgaaaatttg caatctgcgg ctatcatcgg
     4381 cgaaatggtg ggtaaaaagc gagagatgca ggcacgtctg gaacaacata aagagaggat
     4441 ggcgcagtgg gccagccagc ttcccaaagg gacacgcgtg gcctttggca catcacgcga
     4501 acagcaattc aacctgcata ctcaggagac ctggaccggc agcgtgctgg cctctctggg
     4561 gctgaacgtt cccgctgcga tggcgggtgc gtccatgccg tccatcggcc tggagcaact
     4621 gctggcggtc aatcctgcct ggctgctggt tgcccactat cgcgaagaga gcattgttaa
     4681 acgctggcaa caagatccgc tctggcagat gttaaccgcc gcgcagaagc agcaggttgc
     4741 ttcggtcgac agtaacacct gggcgcggat gcgcggtatt tttgctgcag agcgtattgc
     4801 cgctgacacg gtaaaaatct tccaccatca gccgcttacc gttgtgaaat gaccgcgata
     4861 aaacacccgg tgctgctgtg ggggcttccc gttgcagcac ttattattat tttctggctg
     4921 agtctgtttt gctactcggc cattcctgtt tccggagcag atgcaacccg cgccctgctg
     4981 cctggacaca cgccaacgct accagaagcg ctggtgcaaa accttcgttt gccacgaagc
     5041 ctggtcgccg ttctgatcgg cgcaagcctg gcgctcgcgg gcacgctgct gcaaaccctg
     5101 acccacaacc caatggcctc tccttcactg ctcggcatta acagcggcgc ggcactagct
     5161 atggcgctta ccagcgcgct gagtccgacg ccgattgcag gctattctct gtcgttcatc
     5221 gcggcatgcg ggggcggcgt gagctggctg ctggtcatga ccgcaggagg cggatttcgt
     5281 catacccatg acagaaacaa actgatcctc gcgggtatcg cgctgtcggc cttttgtatg
     5341 ggccttaccc gcatcaccca gctgctggcc gaagatcatg cttacggcat cttttactgg
     5401 ctggcaggcg gagtgtccca cgcccgctgg caggatgtct ggcagctctt gccggtggtg
     5461 gtcactgcag tccctgtcgt gttgctgctg gcgaatcaac tgaacctgct caacctcagc
     5521 gacagcaccg cccatacgct gggagtgaac ctgacgaggc tacgtttggt catcaatacg
     5581 ttagtgctgc ttctggttgg tgcgtgcgtc agcgtggcag gtccggtggc gtttatcggt
     5641 ctgctggtgc cacatctggc gcgcttctgg gcaggcttcg atcagcgcaa cgtactgcca
     5701 gtgagcatgc tgctgggggc cacgctgatg ctgctggcag atgtactcgc acgcgcgctg
     5761 gccttccccg gagatctgcc cgcaggcgca gtgctggcgc tgattggcag cccttgcttt
     5821 gtctggcttg tgaggaggcg aggatgaaaa ttgcgctggt tattttcatc acccttgccc
     5881 tggcgggctg tgcgctgtta tcactccata tgggagtgat ccccgtgccg tggcgcgcgc
     5941 tgctgaccga ctggcaggcc ggacgcgagc attattatgt attgatggag taccgactgc
     6001 cgcgcttgct gctggcactg tttgtcggtg cagccctcgc cgtggcgggc gtgctgatac
     6061 aggggattgt gcgcaaccct ctggcatcac cggatattct cggcgttaac catgccgcca
     6121 gcctggcctc tgtgggggct ctacttctta tgccgtcact gcccgtgatg gtgctgccgc
     6181 tgctggcctt tgcgggcggc atggcggggt tgatattact gaagatgctg gcaaagaccc
     6241 accagccgat gaagctggcg ctcaccggcg tggcgctttc tgcatgctgg gccagcctga
     6301 cggattatct gatgctctcg cgcccacagg atgtgaacaa cgccctgctg tggctgaccg
     6361 gcagcttatg gggccgtgac tggagctttg tgaagattgc catcccgctg atgattttat
     6421 ttctgccgct gagcctgagt ttttgccgcg atctcgacct ccttgcactc ggcgatgcgc
     6481 gcgccaccac gctcggtgtg tcggtgcccc atacccgatt ctgggctttg ttactagctg
     6541 tcgccatgac atctaccggc gtggccgcct gcggcccgat tagctttatt ggtctcgtgg
     6601 tgccgcatat gatgcgtagc atcaccggtg gacgtcaccg cagactgctg cctgtttcag
     6661 ccctgacagg tgcgttgctg ttggtggttg ccgatctgct ggcgagaatt attcatcccc
     6721 cactggagct cccggttggc gtgctgaccg ccattatcgg tgcgccgtgg tttgtctggt
     6781 tgcttgtgag aatgcgataa atgactttac gaactgaaaa tctgacggtc agttacggga
     6841 cagacaaggt acttaacgac gtttcactct cactgccaac ggggaagatc accgccctga
     6901 tcggtcctaa cggttgcggg aaatcgacgc tgttaaactg tttttcgcgg cttttaatgc
     6961 cgcagtctgg caccgtattt ctcggcgata atcccataaa tatgctctca tcgcgccagt
     7021 tggcccgcag gctttcgctg ctgcctcagc accatttaac gccagagggg atcacagtcc
     7081 aggagctggt ttcgtatggt cgtaatccct ggctgtcact ctgggggcgt ctctccgctg
     7141 aagacaatgc acgagttaat gtcgccatga accagacccg gatcaatcat cttgccgttc
     7201 gtcggttaac cgagctttcc ggcggtcagc gccagcgcgc atttctggcg atggtcctgg
     7261 cccagaatac gcccgttgta ttacttgatg agccaaccac ctatcttgat atcaatcacc
     7321 aggtggacct gatgcggttg atgggcgaac tccggactca ggggaaaacg gtggtcgctg
     7381 tgctgcacga ccttaatcag gctagccggt actgcgatca actggtggta atggcaaacg
     7441 gacatgttat ggcgcaaggc acaccagaag aggtgatgac cccaggattg ctgagaacag
     7501 tattcagcgt ggaagcggaa atacaccccg agccggtatc tggcaggccg atgtgcctaa
     7561 tgaggtagat tgcacaggcc gtaagaacca aaccacgact gaatgaaact ggactggcgc
     7621 cagcaagcct gttcagactg gggctgaact tttccggact ctgaaagatt accaatactc
     7681 atcgtccatc cgcttgcttt aggctgacag gttcataatc aacgcaaacc agagctgtac
     7741 aggcttgggc gcggctttca aaccagtcgt gatcacggca atcaattttg aactctgctt
     7801 aacggacatt tctgtataac ccttacggca acgaaaaacg cgaagttaaa attttagaaa
     7861 cccaaaaacg tgacatgact aagtttagat ttcagggggg gagatcaaaa aatttcgctc
     7921 tgtgccagag cggacattca cggagctggt tcattaccaa tgaggttggg cttttgagga
     7981 taaatcaatg atcagacgcc aacgtaaatc aaaagcaacc ctggaacgga attgctaatc
     8041 cagtttctga ccatcgattt ttctaaaaag tgtgcgtttg ctactactta ggtaggtgca
     8101 gctttcttaa tcaccggcag ccacgttata caggccagtt gatggatcga ttgttatcaa
     8161 tgatatcttt atgagtcggt gtctcaccca gcttaccgaa gctggcataa agtgaaggca
     8221 gacgggcccg tccttctccc tttttcgcca gagggaaagc gcgaagcatg gtggcagcct
     8281 cccagtcaca acaataggat ggtgtgcacg gctgctgacg ccatgattca gcgatagagc
     8341 cggaaaatac ggggtcaaag ccggtatcat taaccagagt catcgtgacc tgtactgcgg
     8401 ctggatgatc tcctgctact gcaactgcta ggcgaccgcg gctcccttca ggtagtctgt
     8461 tgtggtcaac taaaactggc ctccgcgtta gagtttttcc agtatcggtt ttctgattcg
     8521 tttggtggta acccaccatt atattcgtgc ggtcttagtg cgctgtaata tccaacgata
     8581 tagtccgtta ttgcgtgagc tgcatcgctg aagcttacat agcccgtcgc tggcacccat
     8641 tcgttcttca gactcctgaa gaagcgctcc attgggctgt tatcccagca gtttccacgc
     8701 cgactcatac tctgcctgat ccggtatcgc cacagtatga accgccccgg gtttcctgga
     8761 gagtgtttta tctgtgaact caggctgcca gatcatcgtt tccgatggaa gcataataag
     8821 ctttttctgc ttctgccgga ggagtatggc ccagcctttc cagcaatcgt cgattgttat
     8881 accagtccac ccacgtgagt gtggccagtt ccacttctgc acgttttttc cagctcttac
     8941 ggtgtattac ctccgctttg taaagaccat tgatgctctc cgccatcgcg ttgtcatacg
     9001 agtcgcctgt acttcctgtt gatgccagta atccggcttc cttaagccgc tgtgtgtagg
     9061 ccagcgatac atactgagaa cctttatcac tgtgatggac cgtgccggac ggtcgacggg
     9121 cccataacgc ctgctccagt gcatccagca cgaatgtcgt ttccatggac gatgagaccc
     9181 gccaccccac gatgtatccg gcaaacacat caatgatgaa cgccacatag acgaagcccc
     9241 gccatgtgct gacgtaagta aaatcagcca cccacagctg gtcaggtcgt tctgccacga
     9301 actgacggtt tacgcggtcg cctgcggcaa cggctttccg gctgatggtc gtacggacct
     9361 ttttaccccg gagaacaccg gcaagtccca taaccgccat gagacgtgcc acagtgcatc
     9421 tggccactct gataccttcc cgtaacaact gacgccagac tttacgcaca ccgtatacct
     9481 tgtgattttc atcgtatacg cgctgtatct ctttcttcag ccagtcatcg cgctgcgcac
     9541 gggcactgcg tttatccgga tgatgtcgct gttgctgaca gtggtaatac gttgacgggg
     9601 caatatgcag ttcgctgcat agcggtccga ccccgtactg ctcacgcagc ttatccagca
     9661 gtggcatcat tttttccaga ggcggtcgaa ctccgccttc gcaaaataag cggaagcctg
     9721 gcgaaggata tcgttactgc ggcgcagttc acgattttca cgttccagct ctttcagacg
     9781 ctgacgttca gcggtggtga gccctccatc accgcccccg gtatcccgct catgctggcg
     9841 aacccagaca cgcagagtct ccggcgtaca gccaatcttt ggagcaatgg aacaaattgt
     9901 cgcccattgt gagtcatatt cgctctgact ttccagaacc atacggactg cccgttgacg
     9961 gacttcgggg gaaaaacgag tatttttagt catcctgttt acctctttct caggaagttt
    10021 agtctccagg attcccgggg cggttcagga ggacctggaa ctcctgaatc agcagttggg
    10081 cctgttgagt ctggaaagac aacaggactc gcagcctgtt aatcaacaat ctgaacatga
    10141 acccgaatct gcatcaaggg tgaagcgtga tttatctgaa gtcgagttcg agtcaacaat
    10201 gaaacgtaag cgttttatgt agaacgcctg tgcggtgaac tcaggttccg gggaaatagt
    10261 gtccatcatt gtaatggaca ctatcatggg aaatggctct gtcacatcaa caactacagt
    10321 tgctgaccag ccggatggat gattttttcg caaccagaag acgctgcctc cgatccggct
    10381 tacaacaagt tacaccgtca caaccgggac acgtaacgcc agcgcgcaca tcaactcata
    10441 gcccaccgtt ccggcagcgg cggcgacatc atcaattttg atctccttgc cccacagctc
    10501 aaccggcgta ccaatacccg cctgcgggca aggcgttaaa tcgaccgcca gcatatccat
    10561 cgaaactgtc cctactgtca tggtgcgcac gccgtcaact aaaacagggg tgccggtagg
    10621 cgcatggcgc ggataaccgt cggcgtatcc tgcggcgaca atgccgattc gctgttcatc
    10681 tcgcgcagta tagcgaccgc cgtagcccac acgctcgccc gcttttagcg tctggacacc
    10741 aataatctca ctgcttagcg tcatcaccgg acgtaatccg gtattggcga tatcacgcca
    10801 ctgaccggat ggcgaagcgc catacaaaat aatgccaggc cgaacccagt caaaatgcgc
    10861 ttccgggtgc cacagagtcg ccgccgaatt ggacaacgaa cgccgacact ccagcccttc
    10921 cgccgcctgc tcaatacgcg ccatcgcgct ggaaattcca tcaggatgtt ccgcctcggc
    10981 aaaatgcgac atcagggtca tttcgccaac attcgccatc gcccgcaact gctgccagac
    11041 ggtaagcacg cgatcgggct ggaagcccaa ccgattcatc ccactgttca ctttaagata
    11101 aatatccaac ggggctttta gccgcgcatt ttgcagtgct ttgagctgcc agttgctgtg
    11161 tacgcaggtg gtcaggcggt gctggtcata aatctccaga tcctgagcat ggaaaaatcc
    11221 ttccagcatc aggatcggtc ctttccagcc gcgctcacgt aacgttattg cctcttccag
    11281 gttaagtaat gcaaagccat cggtagcccc gagcgcgctc cagatacgct caataccgtg
    11341 cccgtaagcg ttcgctttta ccaccgacca gacgcgcgcg tgcggcgcgg cctggcggac
    11401 aatggacaga ttctgtttta atgcctgcag atcgaggctg gcctgtatcg gacgggtcat
    11461 ctcgtttcct tagctgtgtg cgccatgtaa atggcccgga cgtgatggcg taaatccacg
    11521 gctgtagcgc gctacgctta gatcctcata tgggatcgct ggcgtgcgac cagagatcag
    11581 atcgcttaac aactgaccgg aaccgcaagc catcgtccag ccgagcgtgc cgtgaccggt
    11641 attcagccac agatttttaa agcgtgtacg cccgacaacc ggcgtgccgt ctggcgtcat
    11701 cgggcgcaga ccagtccaga aagtcgcctg ctcgacatga ccgccgcgtg gatagagatc
    11761 gcgaaccacc atctccagcg tttcacgacg cggttgcaac agctcggtat taaaaccaac
    11821 aatttccgcc attccgccaa cacgaatgcg gttatcgaaa cgggtaatgg cgattttgta
    11881 tgtttcatca agaatggtgg ataccggcgc accatcttcc tgcgcaattg gaatggtcag
    11941 cgagtagcct ttcagcggat acaccggaat atcaacaatg cctttgagca tcgccgtcga
    12001 gtaagaacca aacgccatca catacgcatc ggccttaatc acttcatcgc cacacttcac
    12061 gccgtagatt tgctcgccgt cgcaaagcag ttggtcaacg ggcgtattaa agcggaattt
    12121 aacccccgcc tgctccgcca tccgcgccag attctgggta aatagctgac agtctccggt
    12181 ttcatcattg ggtaactgca ggccgcccgt cagtttgtgc gccacttctg ccagcgcggg
    12241 ctccacttcc gccaggcggc tggattccag cagctgatac ggtacgccgg catcttccag
    12301 cacggcgata tcgcgggtcg cattttcata ctgttgttcg gtacggaaca gttgcagcgt
    12361 cccaccctga cgcccttcat actgaatatt ggtttcggcg cgcaatgctt tcaggcaatc
    12421 acggctgtat tccgccagac gcaccatccg ccctttgttt tccatgtagt ggctggtgtc
    12481 gcagttacgt aacatttgcc acatccattt caactggaat tgtgtaccgt cgagacgaac
    12541 cgccagcggc gcatggcgct ggaacatcca tttaatcgct tttaaaggca cacctggtgc
    12601 cgcccacggt gcagcatatc cgggggagat ttgcccggca ttcgcggcac tggtttccag
    12661 ggcggccccc ggctcccgat caatgacggt gacctcatgt cctgcctgat ttaagtacca
    12721 ggcgctggca acgcctacca caccacttcc cagtatgaca actcgcatag ccactccga
//
LOCUS       DAPGOF010000140        12691 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.141, whole genome shotgun sequence.
ACCESSION   DAPGOF010000140 DAPGOF010000000
VERSION     DAPGOF010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12691
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.141"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(42..1400)
                     /gene="plaP"
                     /locus_tag="RHV13_003528"
     CDS             complement(42..1400)
                     /gene="plaP"
                     /locus_tag="RHV13_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HDV1243028.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(1390..1452)
                     /gene="yoeI"
                     /locus_tag="RHV13_003529"
     CDS             complement(1390..1452)
                     /gene="yoeI"
                     /locus_tag="RHV13_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HDV1243029.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(1667..2596)
                     /locus_tag="RHV13_003530"
     CDS             complement(1667..2596)
                     /locus_tag="RHV13_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV1243030.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(2642..3466)
                     /locus_tag="RHV13_003531"
     CDS             complement(2642..3466)
                     /locus_tag="RHV13_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV1243031.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(3549..3803)
                     /gene="yoeB"
                     /locus_tag="RHV13_003532"
     CDS             complement(3549..3803)
                     /gene="yoeB"
                     /locus_tag="RHV13_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HDV1243032.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKIIELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(3800..4051)
                     /gene="yefM"
                     /locus_tag="RHV13_003533"
     CDS             complement(3800..4051)
                     /gene="yefM"
                     /locus_tag="RHV13_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HDV1243033.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            4335..4385
                     /gene="hisL"
                     /locus_tag="RHV13_003534"
     CDS             4335..4385
                     /gene="hisL"
                     /locus_tag="RHV13_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HDV1243034.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    4361..4484
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            4531..5430
                     /gene="hisG"
                     /locus_tag="RHV13_003535"
     CDS             4531..5430
                     /gene="hisG"
                     /locus_tag="RHV13_003535"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HDV1243035.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            5436..6740
                     /gene="hisD"
                     /locus_tag="RHV13_003536"
     CDS             5436..6740
                     /gene="hisD"
                     /locus_tag="RHV13_003536"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HDV1243036.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPAAD
                     MARRVAEAVERQLAELPRAETARQALSASRLIVTNDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            6737..7807
                     /gene="hisC"
                     /locus_tag="RHV13_003537"
     CDS             6737..7807
                     /gene="hisC"
                     /locus_tag="RHV13_003537"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDV1243037.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEISCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGIREESQRVIDALRAEQV"
     gene            7807..8874
                     /gene="hisB"
                     /locus_tag="RHV13_003538"
     CDS             7807..8874
                     /gene="hisB"
                     /locus_tag="RHV13_003538"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HDV1243038.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            8874..9464
                     /gene="hisH"
                     /locus_tag="RHV13_003539"
     CDS             8874..9464
                     /gene="hisH"
                     /locus_tag="RHV13_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HDV1243039.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            9464..10201
                     /gene="hisA"
                     /locus_tag="RHV13_003540"
     CDS             9464..10201
                     /gene="hisA"
                     /locus_tag="RHV13_003540"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HDV1243040.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLQRLQDYATQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            10183..10959
                     /gene="hisF"
                     /locus_tag="RHV13_003541"
     CDS             10183..10959
                     /gene="hisF"
                     /locus_tag="RHV13_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HDV1243041.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLDDAARILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            10953..11564
                     /locus_tag="RHV13_003542"
     CDS             10953..11564
                     /locus_tag="RHV13_003542"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HDV1243042.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(11661..12641)
                     /gene="wzzB"
                     /locus_tag="RHV13_003543"
     CDS             complement(11661..12641)
                     /gene="wzzB"
                     /locus_tag="RHV13_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HDV1243043.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGPAVPKVSDIQASLIGRYSTA
                     FSALAETLDNQDEAEKLTIEPTVKNQSLPLAVSYVGETPEGAQKQLAKYIQQVDDQVN
                     VELEKDLKDNIALRMKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQIQ
                     QTGEDITQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIESLKVDDLDIH
                     AYRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
BASE COUNT         3149 a         3350 c         3278 g         2914 t
ORIGIN      
        1 caaatgttca ttcgactccg gttaattatt agaaaagatg attatgctac gtcttcgtac
       61 tgcggaaccg ggttacggaa gctcttggtg acgcaagcca ggtaaatcag accgatagca
      121 gcccagatca gacccagaac cattgagctt tcttccaggt taacccacag cgcaccaaca
      181 gtcagcgcac cacacatcgg caggaacaga tactggaagt gatctttcag cgtcttatta
      241 cgcttctcac ggatccagaa ctgcgagatg accgacaggt taacgaaggt gaacgccacc
      301 aacgcaccaa agttaatcag cgctgtagcc attaccaggt cgaagttgat tgccagcagg
      361 gcaatcgcgc caaccaggat gatgttcatc gccggagtac gccatttcgg gtgtacataa
      421 ccgaagaagc ttttcgggaa tacgccgtca cgacccatta cgtacatcag acgcgctacg
      481 cctgcgtgcg ccgccatacc agacgccagt acggtaatgg tggagaagat cagcgcgcca
      541 acctggaacg ctttacccgc aacatacagc atgatttcag gctgtgacgc atccggatct
      601 ttaaagcgag agatatccgg gaagtacagc tgcaggaagt aagttgcaaa gatgaagatc
      661 atgccgccaa tcagcgcggt caggaaaatc gcacgcggga taacgcgctc tgcatctttg
      721 gtttcttccg acaggttgct gatgccgtca aagccggtaa aggagaagca caggattgtc
      781 gccccggtaa tcatcgggat aacatgcgca tcgccagacc agaacggacg agtgctcgcc
      841 agcgtaccag cgccttcacc ttcaaatacg ccataaacaa ccatgcccag aatcaccgcg
      901 atcagcacta cctgcaacac gacgattaca gtgttgaagt tcgctacgga tttcagacta
      961 cgcaggttaa aggcggtcat aaaggccacc agcgccacca caaacatcca cgatgggatg
     1021 gaaggcacca gagcttcaaa atagattttc gccagcagaa tgttgatcat cggcgcgaac
     1081 agatagtcga gcagagaaga ccaacccacc ataaagccga cagtcgggct aatggatttc
     1141 tgggcgtaag tgtatgcaga gccagcagaa ggatagcggc gaaccagctt cccgtagctc
     1201 agagccgtaa acaggatcgc aatcaatgcg aacgcatagg ctgtcggcac atgaccatcc
     1261 gtgaggcctg aaacgatacc aaaagtatca aacagcgtca tcggctgcat ataggcaaga
     1321 cccatcatta caaccggaac taacgtaagc gttttacgca attccacgcg agaggtgttt
     1381 ggagtaacgt tatgcgacat ggtcattctc ctttacggtg ataaccgtcg cgtaagcaaa
     1441 aaattgcccc atttttttgg attcctcagc gacaacaact gtcgattttt agtaaatatc
     1501 tatccggtac gaagcccggc ctcttggtat gaatgattgg tttgaagcaa aaaataaccg
     1561 acgctgatga aacgtcggtt tttagtcatt ttttgacagc ggcgcattgt gcctaaatgg
     1621 gggggaaatg acaagagaat gagaggcttg tcataataat ttttctttaa atggctgatt
     1681 ttccgtcatc agattttgca taaacaccgg cgatcgcact atttgctaaa atttcatccc
     1741 gccaccagcc gctggcaagc cctgcggtct gttcattcca gccaatccag acgggttcaa
     1801 agagagtttg ggcgaccact ttcttctcga ttaacgcgcc actatcgaga aaacgttgcg
     1861 ccagataacg gggtaaatag ccgcagccga ggccgctaat ttgcagctcc agcttggttt
     1921 taaaatcgaa aacggtaatg gcttcttgtt cgtcgagaag ttgtgaggcc gtagaagcgc
     1981 ctgcctgcgc agtgtctccc accacaatcg cccgatatcg cttaatgata cgacggttta
     2041 acggctcttc ttcctgggct agcggatgat gaggcgcgac cgcaaagact tgttccagat
     2101 cgcccaaccg cgagaaacca aactcactgg aagatggtgg ctcatgcatc gcgccaacaa
     2161 taatatccgc tcgcccctga gtcagggcat cccaggaacc accgagtacg ccattgataa
     2221 acttcaggcg cgtcacgcta tgatgttgat aaaaagcttc aatcagtggc gcaagcagag
     2281 aaaaaggaaa cgtgtcgtcc acgccaatca ccagctcgtt ttcccagcct tcatgcagtt
     2341 tgatcgcctg cttttccagt tctcgcacgg tatgcagaac ttcccgccct ttctctaata
     2401 acatttttcc ggtacgggtg aatttagcgc ggtgaccgct acgatcaagc aattgaatat
     2461 taaggtcgct ttccagcttg tgaacggtgt aactcagagc ggacggcgtc ttatagagtt
     2521 tggccgacgc cgccgcaaaa ctgccttctt tttctaaggc atcgaggatc atcaacacat
     2581 ccagcagtgg tttcatacac gcccccttgc agtgcatgcg gcgtcctgct ggcgtggtcg
     2641 tttactccag cggcattacc agcggatcgg gatactggta ttcaaatccc agttcattac
     2701 aaatccgact gccatcaata atcttacctt tgccgctgtc cagactattt ctgaactgcg
     2761 gaggctccag ccccagtaaa cgggccatct gcggatagaa aacattacgc gcagggtgag
     2821 ctggcgcaca tatattatag atgtgtccgc ctttaggtgc ctgtaacaac agagtgatag
     2881 cgccaataac atcttctaaa tggactaaat taacaccatg ttcaccatca ggcgcggttt
     2941 ttccggcaaa gaagcgtccg ggatgacgtc ccggtccgac aagacccgca agacgcagaa
     3001 tatcgaccga agtaccaggt aaattgtgca gccagtcttc gagttcttct aatactcgtc
     3061 cactgttggt taccggatta cgcggggtag tttctttgac cgtgccttgc gcgtcgccat
     3121 agacagacgt cgagctggta aaaataattc ggggaatacg atgggccagc gcgctatcca
     3181 ctaactcttg taccgcttgt aaatagaact catcgccggg gccgctacga cgtgccggaa
     3241 gcgtaatgac cagcgcatcg gcatccatca gggcatccag atcgtcagaa tcgcaaacta
     3301 actcaggctc catgcgaagc agatagctat caatgccact cattcgggcc gcttcgacac
     3361 catcttgtgt ggttttactc ccggtgactt gccagcctcg cgctgaaagt gacatcgcca
     3421 gcggcatgcc taaccaccct aacccgacaa ttgcgacctt tttcatccgt tatctcctga
     3481 tacaccttag atctataagg ctacgctagc gtatcaaaac tgacaattca ttctatgaat
     3541 gaatctgttc aataatgata acgacatgct gcaatgagca gtgaatcgtc ggtaaccgcg
     3601 tataccagac ggtgctcctc tgtaatgcgt cgggaccaga agcctgacaa attatgcttc
     3661 aggggttctg gcttcccctt accttcaaat ggtgttctgc gggtatcttt gataagttca
     3721 atgatctttt taacaattcg cttatctgtt tcctgccagt acagataatc atcccatgac
     3781 tcctcagacc agattagttt cactcaataa tgtccttttc cgttcctttg cctgatttca
     3841 ggctatcgat tgagtccatc aatctccggg cgttagcggg ggagcgcagt agataagccg
     3901 tctcttccag cgagttgtat tcttcgagtg acatcagaac acaagcctct ccattctgac
     3961 gagtaataag gatcggggca tgatcttcaa cggctttcat cattgttgcc gacaaattct
     4021 ggcgcgcttc gctgtagcta attgtacgca tttcaatctc ctcttttgta cagttcattg
     4081 tacaatgata ggcgttaatt aactatttat taattagtct gtagatcaag acattgtcag
     4141 tgagacgaaa aatccaggtt tcgctatttt gggtgccgtc aggttagggg acactcctct
     4201 tagccccctc ctttccccgc tcattcatta aacaaatcca tcgccataaa atatataaaa
     4261 aagcccttgc tttctaacgt gaaagtggtt taggttaaaa gacatcagtt gaataaacat
     4321 tcacagagac ttttatgaca cgcgttcaat ttaaacacca ccatcatcac catcatcctg
     4381 actagtcttt caggcgatgt gtgctggaag acattcagat cttccagtgg tgcatgaacg
     4441 catgagaaag cccccggaag atcaccttcc gggggctttt ttattgcgcg gttgataacg
     4501 gttcagacag gtttaaagag gaataagaaa atgacagaca acactcgttt acgcatagct
     4561 atgcagaaat ccggccgttt aagtgatgac tcacgcgaat tgctggcgcg ctgtggcatt
     4621 aaaatcaatc ttcacaccca gcgcctgatc gcgatggcag aaaacatgcc gattgatatt
     4681 ctgcgcgtgc gtgacgacga cattcccggt ctggtaatgg acggcgtggt agaccttggg
     4741 attatcggcg aaaacgtgct ggaagaagag ctgcttaacc gccgagccca gggtgaagat
     4801 ccgcgctact ttaccctgcg tcgactggat ttcggcggct gccgcctttc gctggcaacg
     4861 ccggttgatg aagcctggga cggtccgctc tccttaaacg gtaaacgtat cgccacctct
     4921 tatcctcacc tgctcaagcg ttatctcgac cagaaaggca tctcttttaa atcctgctta
     4981 ctgaacggtt ctgttgaagt cgccccgcgc gccggactgg cggatgcgat ttgcgatctg
     5041 gtttccaccg gtgccacgct ggaagctaac ggcctgcgcg aagtcgaagt tatctaccgc
     5101 tcgaaagcct gcctgatcca gcgcgatggc gaaatggaag aatctaaaca gcaactgatc
     5161 gacaagctgc tgacccgtat tcagggcgtg atccaggcgc gcgaatcaaa atacatcatg
     5221 atgcacgcac cgactgaacg tctggatgaa gtcatcgccc tgctgccagg tgccgaacgc
     5281 ccaactattc tgccgctggc aggtgatcaa cagcgcgtag cgatgcacat ggtgagtagc
     5341 gaaaccctgt tctgggaaac gatggaaaaa ctgaaagcgc tgggagccag ttcaatcctg
     5401 gtcctgccga ttgagaaaat gatggagtga tcgccatgag ctttaacaca atcattgact
     5461 ggaatagctg tactgcggag caacaacgcc agctgttaat gcgcccggcg atctccgctt
     5521 ctgaaagcat tacccgcact gttaacgata ttctcgataa cgtgaaagca cgcggtgatg
     5581 acgccctgcg ggaatacagc gcgaagtttg ataaaaccac ggttaccgcg ctgaaggtgt
     5641 ctgctgagga gatcgccgcc gccagcgaac gcctgagcga cgagctaaaa caggcgatgg
     5701 cggtggcagt aaagaatatt gaaaccttcc acactgcgca aaaactgccg ccggtagatg
     5761 tagaaacgca gccaggcgta cgttgccagc aggtcacgcg tccggtagct tcagttgggt
     5821 tgtatattcc tggcggctcc gccccgctct tctcaacggt attaatgctg gcaactccgg
     5881 cgcgtattgc gggctgtaaa aaagtggtgc tgtgctcacc accgccgatt gccgatgaga
     5941 tcctttacgc ggcgcagctg tgcggcgtgc aggacgtgtt taacgtcggc ggcgcacagg
     6001 ccattgccgc gctggcgttt ggtacagaat ccgtgccgaa agtggacaaa atcttcgggc
     6061 caggtaacgc ctttgtcacc gaggcaaaac gtcaggtgag ccagcgtctg gacggtgcgg
     6121 cgatcgatat gcccgcaggc ccgtcggaag tgctggtgat tgctgacagc ggcgcaacgc
     6181 cggatttcgt ggcttctgat ttgctctccc aggctgaaca cggcccggac tctcaggtga
     6241 ttttactgac gcccgctgct gatatggcgc gtcgcgttgc cgaagctgtc gaacgccagc
     6301 tggcagaact gccgcgagct gaaaccgcac gtcaggcact gagcgccagc cgcctgatcg
     6361 tgaccaacga tttagcgcag tgcgtagaga tctctaacca gtacggcccg gagcacctga
     6421 tcattcagac ccgcaacgcc cgcgatctgg tcgatggcat caccagcgcc ggttcggtat
     6481 ttctgggtga ctggtccccc gaatcggcgg gtgattacgc ctccggcact aaccacgtac
     6541 tgccgactta cggttacacc gccacctgtt ccagcctcgg gctggcagat ttccagaagc
     6601 gtatgaccgt gcaggaactg tcgaaagaag ggttctccgc gctggcttca accatagaaa
     6661 cactggccgc cgccgagcgc ctgaccgccc acaaaaatgc cgttactttg cgtgttaacg
     6721 cccttaagga gcaagcatga gcaccgtgac tattaccgat ttagcgcgtg aaaacgtccg
     6781 caacctgacg ccgtatcagt cagcgcgccg tctgggcggt aacggcgacg tctggctgaa
     6841 cgccaacgaa taccccacag ccgtggagtt tcagcttact cagcaaacgc tcaaccgcta
     6901 cccggaatgt cagccgaaag cggtgattga aaattatgca cagtatgcag gcgtgaaacc
     6961 ggaacaggtg ctggttagcc gtggcgcgga cgaaggtatt gaactgctga ttcgcgcttt
     7021 ttgcgaacca ggtaaagacg ccatcctcta ctgcccgcca acgtacggca tgtacagcgt
     7081 cagcgctgaa accattggcg tcgagtgccg cacagtgccg acgctggaca actggcaact
     7141 ggacttgcag ggcatttccg acaagctgga cggcgtaaaa gtggtctatg tttgcagccc
     7201 caacaacccg accgggcaac tgatcaatcc gcaggatttt cgcaccttgc tggagttaac
     7261 ccgcggaaaa gccattgtgg ttgccgatga agcctatatc gagttttgcc cacaggcatc
     7321 gctggctggc tggctggcgg aatatccgca cctggctatt ttacgcacac tgtcgaaagc
     7381 ttttgctctg gcgggacttc gttgcggatt tacgctggca aacgaagaag tcatcaacct
     7441 gctgatgaaa gtgattgccc cctacccgct ctcgacgccg gttgccgaca ttgcggccca
     7501 ggcgttaagc ccgcagggga tcgtcgccat gcgcgaacgg gtggcgcaaa ttattgcaga
     7561 gcgcgaatac ctgattgccg cactgaaaga aatctcctgc gtggagcagg tttttgactc
     7621 tgaaaccaac tacattctgg cgcgctttaa agcctccagc gcagtgttta aatctttgtg
     7681 ggatcaaggc attatcttac gtgatcagaa taaacaaccc tctttaagcg gctgcctgcg
     7741 aattaccgtc ggaatccgtg aagaaagcca gcgcgtcatt gacgccttac gtgcggagca
     7801 agtttgatga gtcagaagta tctttttatc gatcgcgatg gaaccctgat tagcgaaccg
     7861 ccgagtgatt ttcaggtgga ccgttttgac aaactcgcct ttgaacctgg cgtgatcccg
     7921 gagctgctga agctgcaaaa agcgggctac aagctggtga tgatcactaa tcaggatggt
     7981 ctgggaacac aaagtttccc gcaggcggat ttcgatggcc cacacaacct gatgatgcag
     8041 atcttcacct cacaaggcgt gcagtttgat gaagtgctga tttgtccgca cctgcccgcc
     8101 gatgagtgcg actgccgtaa gccgaaagta aaactggtgg agcgttatct cgctgagcaa
     8161 gcgatggatc gcgccaacag ttatgtgatt ggcgatcgcg cgaccgatat tcaactggct
     8221 gaaaacatgg gtattaatgg tttacgctac gaccgcgaaa ccctgaactg gccaatgatt
     8281 ggcgagcaac tcactaaacg agaccgttac gcccatgtag tgcgcaacac caaagagacg
     8341 caaattgacg tccaggtgtg gctggatcgt gaaggtggca gcaagattaa taccggcgtt
     8401 ggattctttg atcacatgct ggatcagatc gccacccacg gcggtttccg gatggaaatc
     8461 aacgttaaag gcgacctcta tatcgacgat caccacaccg tcgaagatac cggcctggcg
     8521 ctgggcgaag cattaaaaat tgctcttggc gacaaacgcg gtatttgccg ctttggtttt
     8581 gtgctgccga tggacgaatg ccttgcccgc tgtgcactgg atatctctgg tcgcccgcac
     8641 ctggaatata aagctgaatt tacctaccag cgcgtgggcg atctcagcac tgaaatgatc
     8701 gagcacttct tccgttcgct ctcttacacc atgggcgtga cgctacacct gaaaaccaaa
     8761 ggtaaaaacg atcaccaccg tgtagagagc ctgttcaaag cctttggtcg caccctgcgc
     8821 caggccatcc gcgtggaagg cgacaccctg ccctcgtcga aaggagtgct gtaatgaacg
     8881 tggtgatcct tgataccggc tgcgccaatt tgaactcggt gaagtcagcc attgcgcgtc
     8941 acggttatga acccaaagtc agccgtgacc cggacgtcgt gttgctggcc gataaactat
     9001 ttttacccgg cgttggcacc gcacaagcag cgatggatca ggtccgtgag cgcgagctgt
     9061 ttgatctcat caaagcctgt acccaaccgg tgctgggcat ctgcttaggg atgcaattgc
     9121 tggggcggcg cagcgaagag agcaacggcg tcgatttgct gggcatcatc gacgaagacg
     9181 tgccgaaaat gaccgacttt ggtctgcccc tgccgcatat gggctggaac cgcgtttacc
     9241 cgcaggcagg caaccgcctg tttcagggga ttgaagacgg cgcgtacttt tactttgttc
     9301 acagttacgc aatgccggtc aatccgtgga ccattgccca gtgtaattac ggcgaaccgt
     9361 tcaccgcggc ggtacaaaaa gataacttct acggcgtgca gttccacccg gagcgttctg
     9421 gtgccgctgg tgctaagttg ctgaaaaact tcctggagat gtgatgatta ttccggcatt
     9481 agatttaatc gacggcactg tggtgcgtct ccatcagggc gattatggca aacaacgcga
     9541 ttacggtaac gacccgctgc aacgcttaca ggattacgcc acacaaggtg ctgaagtgct
     9601 gcaccttgtg gatctgaccg gggcaaaaga tccggctaaa cgtcaaatcc cgctgattaa
     9661 aaccctggtc gcgggcgtta acgttccggt gcaggttggc ggcggcgtgc gtagcgaaga
     9721 agacgtggcg gcgttactgg aagcaggcgt tgctcgcgtg gtggttggct ccaccgcggt
     9781 gaaatcacca gaaatggtga aaggctggtt tgaacgtttc ggtgccgatg ccttagttct
     9841 ggcgctggat gtccgtattg acgagcaagg caacaaacag gtggcagtca gcggctggca
     9901 agagaactcg ggcgtttcac tggaacaact ggtggaaacc tatctgcccg tcggcctgaa
     9961 acatgtgctg tgtaccgata tctcacgcga cggtacgctg gcaggctcta acgtctcttt
    10021 atatgaagaa gtgtgcgcca gatatccgca ggtggcattt cagtcatccg gcggcattgg
    10081 cgacattaac gatgttgctg ccctgcgtgg cactggcgtg cgcggcgtaa tagttggtcg
    10141 tgcattactg gaaggtaaat tcaccgtgaa ggaggccatc gcatgctggc aaaacgcata
    10201 attccatgtc tcgacgttcg tgatggtcag gtggtgaaag gcgtacagtt tcgcaaccat
    10261 gaaatcattg gcgatatcgt gccgctggca aaacgctacg ctgaagaagg cgctgacgaa
    10321 ctggtgttct acgatatcac cgcttccagc gatggccgtg tggtagataa aagctgggta
    10381 tctcgcgtgg cggaagtgat cgacattccg ttctgcgtgg cgggtgggat taaatcgctg
    10441 gatgacgcag ccagaattct ttcttttggc gcggataaaa tttccatcaa ctcccctgcg
    10501 ctggcggacc caacgttaat tactcgcctg gcggatcgct ttggcgtgca gtgtattgtg
    10561 gtcggcattg atacctggta cgacgccgaa accggtaaat atcatgtgaa tcaatatacc
    10621 ggcgatgaaa gccgcacccg cgtcactcag tgggaaacgc tcgactgggt agaggaagtg
    10681 caaaaacgcg gtgccggaga aatcgtcctc aacatgatga atcaggacgg cgtgcgtaat
    10741 ggttacgacc tcgaacagct gaaaaaagtg cgtgaagttt gccacgtccc gctgattgcc
    10801 tccggtggcg cgggcaccat ggaacacttc ctcgaagcct tccgcgatgc cgacgttgac
    10861 ggcgcgctgg cagcttccgt attccacaaa caaataatca atattggtga attaaaagcg
    10921 tacctggcga cacagggcgt ggagatcagg atatgttaac agaacaacaa cgtcgcgaac
    10981 tggactggga aaaaaccgac ggactgatgc cggtgattgt gcaacacgct gtatccggcg
    11041 aagtgttaat gctgggctat atgaacccgg aagccttaga caaaaccatc gaaagcggca
    11101 aagtcacctt cttctcgcgc actaaacagc gactgtggac caaaggcgaa acgtcgggca
    11161 atttcctcaa cgtagtgagt attgcgccgg actgcgacaa cgacacttta ttagtgctgg
    11221 cgaatcccat cggcccgacc tgccacaaag gcaccagcag ctgcttcggc aacaccgctc
    11281 accagtggct gttcctgtat caactggaac aactgctcgc cgagcgcaaa tctgccgacc
    11341 cggaaacctc ctataccgcc aaactgtatg ccagcggcac caagcgcatt gcgcagaaag
    11401 tgggggaaga aggggtggaa actgcgctgg cagcaacggt acatgaccgc tttgagctga
    11461 ccaacgaggc atctgacttg atgtatcacc tgctggtgtt gttgcaggat caggatctgg
    11521 atttaacgac ggtgattgag aacctgcgta aacggcatca gtgagttgcg gggtaagcgg
    11581 atgcgatgtt gttgtcgcat ccggcaaaaa aaacgggcaa ggtgtcacca ccctgccctt
    11641 tttctttaaa accgaaaaga ttacttcgcg ttgtaattgc gtagagcatt acgccccagc
    11701 acaatccccg cgccaaccat gccgcccagc agcactgcca gaatcaatgt aattgccttt
    11761 ttcggactat cgcgacgaat aggtaacgtc ggtttcatca cataacggta agcatgaata
    11821 tcaagatcat caacttttaa gctttcgata tccagcaggt tttgacgagt ctgatagtag
    11881 tttggtgaga acaccaacgg acgggtcgcc tcatgcttaa tcatcgactc cagcgcttcg
    11941 ctccccaaaa ggaacaacgt atcttgcgtg atatcttcgc cagtctgttg aatttgcggt
    12001 tttgtcacct gcgcctgatt cgcatactgc aacgcttcct gaatctgacg gatacgcaga
    12061 tctttctgct cttgcgcgac tacttcctga gttcttaaag agtcctgcaa gtttttcata
    12121 cgcagcgcga tattgtcttt caagtctttt tccagctcta catttacctg gtcgtcaact
    12181 tgctggatat acttcgccag ctgcttctgc gcaccttcag gtgtttcacc aacataagac
    12241 accgctaaag gtaatgactg atttttgacg gtaggctcaa tggtcagctt ttctgcttca
    12301 tcctggttat ccagcgtttc cgctaatgct gagaatgcag tgctgtaacg tccaataagt
    12361 gaagcctgaa tgtcggagac tttcggtaca gccggaccgt aaatcacatt catcgcgttg
    12421 gtataaccag cgatttgccc cacatcaggt tgagtaacaa tagctgtgga agtccacttt
    12481 tctttggcaa cggccagata ccctatagcc agggcaatag ccacaatgac agaaatgatg
    12541 atagtcatct tgccgcgcca caactgcacc agtaaatcaa tcaaatcaat ctgttccggg
    12601 tcatggtttt gcccagaaac attattattt tctactctca tcattaccct aacggacgaa
    12661 ataattttaa gtcgccatag tttagcgata a
//
LOCUS       DAPGOF010000141        12635 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.142, whole genome shotgun sequence.
ACCESSION   DAPGOF010000141 DAPGOF010000000
VERSION     DAPGOF010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.142"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            92..1606
                     /gene="caiT"
                     /locus_tag="RHV13_003544"
     CDS             92..1606
                     /gene="caiT"
                     /locus_tag="RHV13_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HDV1243044.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1637..2779
                     /gene="caiA"
                     /locus_tag="RHV13_003545"
     CDS             1637..2779
                     /gene="caiA"
                     /locus_tag="RHV13_003545"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HDV1243045.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            2908..4125
                     /gene="caiB"
                     /locus_tag="RHV13_003546"
     CDS             2908..4125
                     /gene="caiB"
                     /locus_tag="RHV13_003546"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HDV1243046.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4199..5752
                     /gene="caiC"
                     /locus_tag="RHV13_003547"
     CDS             4199..5752
                     /gene="caiC"
                     /locus_tag="RHV13_003547"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HDV1243047.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLLTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5861..6646
                     /gene="caiD"
                     /locus_tag="RHV13_003548"
     CDS             5861..6646
                     /gene="caiD"
                     /locus_tag="RHV13_003548"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HDV1243048.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6652..7242
                     /gene="caiE"
                     /locus_tag="RHV13_003549"
     CDS             6652..7242
                     /gene="caiE"
                     /locus_tag="RHV13_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HDV1243049.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRTVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7361..7756)
                     /gene="caiF"
                     /locus_tag="RHV13_003550"
     CDS             complement(7361..7756)
                     /gene="caiF"
                     /locus_tag="RHV13_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HDV1243050.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(8017..11238)
                     /gene="carB"
                     /locus_tag="RHV13_003551"
     CDS             complement(8017..11238)
                     /gene="carB"
                     /locus_tag="RHV13_003551"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDV1243051.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11256..12404)
                     /gene="carA"
                     /locus_tag="RHV13_003552"
     CDS             complement(11256..12404)
                     /gene="carA"
                     /locus_tag="RHV13_003552"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDV1243052.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT         2944 a         3294 c         3354 g         3043 t
ORIGIN      
        1 acgaggttaa taataattat attaaatgtt aacaaaaata aaacaaacgg gaacgcaaaa
       61 taaatattcg ttttcacagt ggaattaact catgaagaat gaaaagagaa aaacgggaat
      121 agaaccgaag gttttctttc cgccgttaat aatcgtcggc atactttgtt ggcttacagt
      181 cagagatctg gatgcggcga atgtcgttat taatgctgta ttcagttacg tcaccaatgt
      241 atggggatgg gcatttgaat ggtatatggt ggtgatgctt ttcggttggt tctggctggt
      301 gtttggcccg tatgccaaaa agcgtttagg taacgaaccg ccagaattta gcaccgccag
      361 ttggatcttt atgatgttcg cctcctgtac atctgctgcc gtactgttct ggggatcgat
      421 tgagatctac tactacatct ccaccccgcc gtttggctta gaaccgaact cgacaggagc
      481 gaaagagttg gggctggctt acagcttgtt ccactgggga cctctgccgt gggccactta
      541 cagcttcctt tcagtcgcct tcgcttactt cttctttgtc cgcaaaatgg aagtgattcg
      601 ccccagctcg acactggtgc cgctggtagg tgaaaaacac gccaaagggt tgttcggtac
      661 catcgtcgac aacttctatc tcgtcgcctt gatcttcgcg atgggcacca gtctgggcct
      721 tgccacgccg ctggtgaccg agtgtatgca atggttgttt ggcattccgc ataccctgca
      781 actggacgct atcatcatta cctgctggat tatcctcaat gccatttgcg tcgcctgcgg
      841 tctgcaaaaa ggggtacgta tcgccagtga cgtgcgtagt tacctgagct tccttatgct
      901 aggttgggtg ttcattgtca gcggtgccag cttcatcatg aactacttca ccgattcggt
      961 ggggatgttg ctgatgtatc tgccgcgaat gttgttctat accgatccca tcgctaaagg
     1021 cggcttcccg cagggctgga ccgtgttcta ctgggcatgg tgggtgattt acgccatcca
     1081 gatgagtatc ttcctcgccc gcatctcccg tggtcgtact gtgcgtgaac tgtgcttcgg
     1141 catggtgatg gggctgacag catccacctg gatcctgtgg accgtgctcg gtagtaacac
     1201 tctgctgttg atggataaaa acatcatcaa cattccaaat ctgatcgaac agtacggtgt
     1261 ggcgcgcgcc atcatcgaaa cctgggccgc tctgccactc agcaccgcca ccatgtgggg
     1321 cttcttcatc ctctgcttta ttgccaccgt tacgctggtt aacgcctgct cttataccct
     1381 ggcgatgtcc acttgccgcg aagtacgcga tggtgaagaa ccacctctgc tggtgcgtat
     1441 cggttggtca attctggttg gcattatcgg tattgttctg ctggcgctcg gcggcctgaa
     1501 accgattcaa accgccatta tcgccggagg atgcccgctg ttcttcgtca acattatggt
     1561 gacgctctcc tttattaaag acgcgaaaca gaactggaaa gattaattaa cccccaaaat
     1621 atcaagaggt tgaaagatgg attttaattt aaatgatgag caggaactgt ttgtcgccgg
     1681 tatccgcgaa ctgatggcca gcgaaaactg ggaggcctat tttgccgagt gcgaccgtga
     1741 cagcgtctac ccggaacgtt ttgtcaaagc actggcggat atgggtatcg acagtctgct
     1801 gatccctgaa gagcacggtg gtctggacgc ggggtttgtt actctcgccg ccgtgtggat
     1861 ggagctggga cgtctggggg caccaaccta tgtgctgtac cagttgccgg gcgggttcaa
     1921 caccttcctg cgcgaaggca cacaagagca gatcgacaag attatggctt tccgcggcac
     1981 cggtaagcag atgtggaact cagcgattac cgaaccgggc gcgggctccg acgtgggtag
     2041 cctgaaaacg acttataccc gtagaaatgg taagatttat cttaatggta gtaagtgttt
     2101 tattaccagc agcgcctaca ccccgtacat cgtggtgatg gcgcgcgacg gggcttctcc
     2161 ggacaaacct gtctacaccg aatggtttgt tgatatgagc aaaccgggca tcaaagtgac
     2221 caaacttgaa aagctcggtc tgcgtatgga tagctgctgt gaaatcacct ttgacgacgt
     2281 ggaactggac gagaaagaca tgttcggtcg ggaaggtaac ggctttaacc gcgtcaaaga
     2341 agagttcgac catgaacgtt tcctggtagc cctcaccaac tacggtacgg cgatgtgcgc
     2401 ctttgaagat gcggcgcgct acgccaatca gcgcgtgcag tttggcgagg ctattggtcg
     2461 tttccagttg attcaggaaa aattcgccca catggcgatc aaattaaact ccatgaaaaa
     2521 catgctgtat gaagcagcgt ggaaagcaga caacggcacc atcacctctg gcgatgcagc
     2581 gatgtgcaaa tacttctgcg ccaatgcggc atttgaagtt gtggatagcg caatgcaggt
     2641 gctgggcggt gtcgggattg cgggcaacca ccgcatcagc cgcttctggc gtgacctgcg
     2701 tgtagaccgc gtctccgggg gatctgacga aatgcagatc ctgacgctgg gtcgcgcggt
     2761 gctgaagcaa taccgctaag tccgtgcgct gcctgatgcg acgctaatgc gtcttatcag
     2821 gcctacggga gcacgaatgt aggccggata aagcgtttac gccgcatccg gcagtcatgc
     2881 gctacataaa tttttcagga gctaataatg gatcatctac ccatgccgaa attcgggccg
     2941 ttggccggat tgcgcgttgt cttctccggt atcgaaatcg ccgggccgtt cgccgggcaa
     3001 atgttcgcag aatggggtgc ggaagttatc tggatcgaga acgtcgcctg ggccgacacc
     3061 attcgcgttc aaccgaacta cccgcagctc tcccgccgca atttgcacgc gctgtcgtta
     3121 aatattttca aagatgaagg ccgcgaagcg tttctgaaat taatggaaac caccgatatc
     3181 ttcatcgaag ccagtaaagg tccggccttt gcccgtcgtg gcattaccga tgaagtactg
     3241 tggcagcaca acccgaaact ggttatcgct cacctgtccg gttttggtca gtacggcacc
     3301 gaggagtaca ccaatcttcc ggcctataac accatcgccc aggccttcag tggttacctg
     3361 attcagaacg gtgatgttga ccagccaatg cctgccttcc cgtataccgc cgattacttt
     3421 tctggcctga ccgccaccac ggcggcgctg gcagcactgc ataaagtgcg tgaaaccggt
     3481 aaaggcgaaa gtatcgacat cgccatgtat gaagtgatgc tgcgtatggg ccagtacttc
     3541 atgatggatt acttcaacgg cggcgaaatg tgcccgcgca tgagcaaagg taaagatccc
     3601 tactacgccg gttgcggtct gtataaatgc gccgacggct acatcgtgat ggaactggtg
     3661 ggcattaccc aaattgaaga gtgctttaaa gatattggcc tcgcacatct gctcggtacg
     3721 ccggaaatcc cggaaggcac tcagcttatc caccgtatcg aatgccctta cggcccactg
     3781 gtagaagaga aactcgatgc ctggctggcg gcacatacca tcgcagaagt taaagaacgc
     3841 tttgccgaac tgaatatcgc ctgtgccaaa gtactgaccg taccggaact ggaaagcaat
     3901 ccacagtatg ttgcccgtga atcaatcact cagtggcaaa cgatggatgg tcgcacctgc
     3961 aaagggccga acatcatgcc gaaattcaaa aataaccccg gacaaatctg gcgcggaatg
     4021 ccctcacatg gcatggacac ggctgccatt ttgaaaaata tcggctacag cgaaaacgac
     4081 attcaggagt tggtcagcaa aggtctggcc aaagttgagg actaaacgcg tggcgtcagt
     4141 cggcttgcct gactagcgcc cgcccaataa caaacgaaca aaaatggata gaggtgcaat
     4201 ggatatcatt ggcggacaac atctacgtca aatgtgggac gatctggcgg acgtttacgg
     4261 tcataaaacg gcgctgattt gtgaatccag cggcggagtc gttaaccggt atagttatct
     4321 tgagttaaat caggagatta accgcacggc aaacctgttt tatacgctgg ggattcgcaa
     4381 aggcgacaag gttgcactac atctcgacaa ctgcccggaa tttatctttt gctggttcgg
     4441 gctggcaaaa attggcgcga ttatggtgcc gattaacgcc cgcctgttac gcgaggaaag
     4501 cgcgtggatc ctgcaaaata gccaggcgtg cctgctggtg accagtgcgc aattctatcc
     4561 tatgtatcaa cagattcagc aggaagatgc cactcaattg cggcacattt gcctgacaga
     4621 tgtggcactt cccgctgatg atggcgtgag ttcgtttact caactgaaaa atcaacaacc
     4681 tgccaccttg tgctatgcac cgccgctatt gactgacgat acggcggaaa ttctcttcac
     4741 ctccggcacc acctcccgac cgaaaggtgt ggtgattacc cattacaacc tgcgcttcgc
     4801 tggatattac tccgcctggc agtgtgcact gcgtgacgat gacgtctacc tgacggtaat
     4861 gcctgcgttt catatcgatt gccagtgtac tgcggcgatg gcggcgtttt ctgccggggc
     4921 cacctttgtg ctggtcgaga aatacagcgc ccgcgccttc tggggacagg tgcagaagta
     4981 ccgcgccacc attaccgaat gtattccgat gatgatccgt acgttgatgg tgcagccgcc
     5041 ttcagcgaac gatcggcaac accgcctgcg ggaagtgatg ttttatctca acttgtcgga
     5101 gcaggaaaaa gatgcgtttt gtgaacgctt cggcgttcgc ttgctgacgt cttatgggat
     5161 gacggaaacc attgtgggca ttatcggcga tcgccctggc gataaacgac gctggccgtc
     5221 gattggtcgg gcggggtttt gctacgaagc ggagatccgc gacgatcaca atcgcccgct
     5281 cccggcaggt gagatcggtg aaatctgtat taaaggcgta ccagggaaaa ccatcttcaa
     5341 agagtatttt ctcaacccga aagccactgc gaaagtgctg gaagccgacg gctggctaca
     5401 taccggcgat accggatact gcgacgaaga gggctttttt tatttcgtcg atcgccgctg
     5461 caatatgatc aaacgcggtg gcgagaatgt ctcctgcgtg gagctggaaa atatcatcgc
     5521 cacccatcca aaaattcagg atatcgtggt tgtgggtatt aaagattcga ttcgcgatga
     5581 agccatcaaa gcatttgtgg tgctgaatga aggtgaaaca ttgagcgaag aggaattttt
     5641 ccgcttctgc gaacaaaata tggcgaaatt taaagtgccc tcttatctgg agatcagaaa
     5701 agatctgcca cgtaattgct cggggaaaat aattagaaag aatctgaaat aaatgaaacg
     5761 gcagggaact accctgccag ctaataatca cgctctaaaa caatatgcat tttttgcagg
     5821 gatgttgtcg tccctgaaaa agcaaaaatg gagaaaagga atgagtgaat cattacatct
     5881 gacccgcaat ggatcaattc tggaaattac ccttgatcgc ccaaaagcga atgctattga
     5941 tgcaaaaacc agctttgaaa tgggcgaagt atttctaaat ttccgtgacg atccgcaatt
     6001 acgtgtcgcc attattaccg gtgccggaga gaaattcttt tccgcgggct gggatttaaa
     6061 agcggcagca gaaggcgaag caccggatgc tgactttggc ccgggtggtt ttgcgggatt
     6121 aaccgaaatt ttcaatctcg acaaaccggt tatcgcagct gtgaacggct acgcctttgg
     6181 cggcggtttc gaactggcgc tggcggcaga ttttattgtt tgtgccgata acgccagctt
     6241 cgccctgccg gaagccaaac tgggcatcgt tcctgacagc ggcggtgtgc tgcgtctgcc
     6301 gaagatcctg ccgcctgcca tcgtcaatga aatggtgatg accggcagac gaatgggcgc
     6361 agaagaggcg ctgcgttggg ggatagtcaa ccgcgtggtt agccaggcgg aactgatgga
     6421 taacgcccgc gaactggctc agcagctggt taacagcgcc ccgctggcga ttgcggcgct
     6481 gaaagagatc taccgcacca ccagcgaaat gccggtagaa gaagcgtatc gctatattcg
     6541 cagcggcgtg ttgaaacact atccatcggt tctgcattcg gaagatgcca ttgaagggcc
     6601 gctggcgttt gccgagaagc gcgatccggt gtggaaagga cgttaacaac cgtgagctat
     6661 tacgcctttg aggggttaat tccggtggtt cacccgacgg cgtttgtcca tcccagtgcc
     6721 gtcttgattg gcgatgtgat tgtgggagcc ggtgtctaca tcggcccact cgcctcactg
     6781 cgtggtgact acgggcggtt gatcgtgcaa gcgggagcca atattcagga tggctgcatt
     6841 atgcatggct actgcgacac tgacaccatc gttggggaaa acggccatat cgggcacgga
     6901 gcgatcctgc atggttgtgt gattggtcgc gatgcattgg tcgggatgaa cagcgtgatt
     6961 atggatggtg cggtcattgg cgaagagagc attgttgccg caatgagctt tgtcaaagcg
     7021 ggctttcacg gcgagaaacg tcagttgttg atgggtacgc cagcccgcgc ggtacgcact
     7081 gttagtgacg acgagttaca ctggaaacgg ttgaatacca aagagtatca ggatcttgtc
     7141 gggcgctgtc atgcatcgtt acatgaaaca cagccactga ggcaaatgga ggaaaatcgc
     7201 ccccgtttgc aggggacgac ggatgtgacg cctaaacggt gatcttgccg gatgcggcgt
     7261 aaacgcctta tccaactctg ctggagagca accgtaggcc ggataagatg cgccagcatc
     7321 gcatccggcg accaggtcgc acggtatcat cgaaatgaca ttaacgacgc atactctttg
     7381 acaacatcat ctgccacttc tgctcgcggt taagttccgt gagcggaacg gcaggaatac
     7441 gcggtgtctt tcttacgccg actaattttt cccgactgtt attgcgcagg cgcgcgtaaa
     7501 tttgctcatc gatatcgacc actttaacca gtcgctgaca ctggcatccc cgcccttcca
     7561 gcttattagg gatcatctta acttcgcagc ttatttctgt gacttccgac agaatataag
     7621 tcagggtatt aaccgccttg ctgtgttcaa tatcgaaatg aatagagatc tctcttgcta
     7681 tcacccaccg attttcagcc atcatccatt cggcgattaa caagtagagt ggtttttcaa
     7741 catatccttc acacatacga actccagttt cacaaattaa aagtctggct atgactcgtt
     7801 ttgaagcaat taaacaaggc catattaaaa ttgtggatat atcgatagta tgcaacttca
     7861 ccgtcgataa gtgtgagcta aatcctgctt atgtcatcaa aaatatcgat tccccaaaag
     7921 atttacttaa tgggcaaaaa aaacggtcct catcagagaa ccgtattagt tattcgatca
     7981 aattagcgga tgaaaaatat ccgccatgac acgctattat ttgatctgcg cgtgcatttc
     8041 ctgcaccgaa attacttttt cggtcgcatc ggcattcagc gccatcgcgg tggcaaatcc
     8101 gccgttcagg gtggtgtcgt aatgcacttt atattgcagc gcactgcgac ggatcacgcg
     8161 ggagtcttca atcgcacgac gacctgaagt ggtgttgatg atgtaggtat attcgccatt
     8221 cttgatgcgg tcctgaatgt gcggacgacc ttcatgcacc ttgtttacca gacgcggatt
     8281 gatacccgct tcgcccagca caatcgccgt gccgtgggtc gcatccagct cgaagccctg
     8341 tttcagcagt tttgccgcca ggtccaccac gcgttcttta tcgccttcgc gcacggaaag
     8401 cagcgcacga ccgtgtttct tcatggtgga gttgctgccc agctgcgctt tggcaaacgc
     8461 ttcagcgaag gtgcggccca cgcccatgac ttccccggta gagcgcattt ctggccctaa
     8521 cagcgggtca acgcccggga atttattgaa cggcagcacc acttctttca ccgagtagta
     8581 cggcgggata acttctttgg ttacgccctg ctcagctagc gatttgccag ccatcacacg
     8641 cgccgccact tttgccagcg gtacgccggt ggctttggag acgaacggaa cggtacgcgc
     8701 cgcacgcggg ttaacttcaa tcaggtagac ttcgttgttt ttcaccgcaa actgcacgtt
     8761 catcagaccg cgcacctgca attcgaaggc cagtttctgc acctgctggc gcatcacatc
     8821 ctgaatttcc tgacttaagg tgtaggctgg cagagaacat gcggagtcac cggagtgcac
     8881 gcccgcctgc tcaatatgct ccatgatgcc gccaatcagc accatttcgc cgtcgcagat
     8941 ggcatccacg tcaacttcta ccgcgtcatc gaggaagtgg tccagcaaca ctggcgcatc
     9001 gttagacacg ctgaccgccg tctggaagta gcgacgcagg tcagcttcgt catagacgat
     9061 ttccatcgcc cgaccgccga gaacgtaaga cggacgtacc accagcgggt agccaatctc
     9121 tttcgccttc tctaccgcca tttcaatagc ggtaacggtg gcgttcgccg gttgtttcag
     9181 tttcagacgg tcaaccgcat gctggaagcg ttcacggtct tctgcacggt cgatagcatc
     9241 cgggctggtg ccgataaccg gtacgccagc ggcttccagc gcacgcgcca gtttcagcgg
     9301 ggtctgaccg ccgtactgga cgataacgcc tttcggcttc tcgatacgca cgatttccag
     9361 cacatcttcc agagttaccg gctcgaagta gaggcgatcg gaagtgtcgt agtcggtaga
     9421 gacggtttcc gggttacagt taaccataat ggtttcgtaa ccgtcttcac gcagcgccag
     9481 cgaggcgtgt acgcagcagt agtcgaattc gataccctga ccgatacggt ttggaccgcc
     9541 gccaagcacc atgatttttt cacggtcggt agacggattc gcttcgcact cttcttcata
     9601 agtggagtac atgtaagcgg tgtcggtggc aaactctgcc gcacaggtat ccacgcgctt
     9661 gtagaccggg tgcaggtcat actggtcacg cagcttacgg atttccgctt cgcgtacgcc
     9721 cgccagtttt gccaagcgcg catcggcaaa gcctttgcgt ttcagctggc gcaggaattc
     9781 agcgttcagg ccagtgatgc ccacttccgc cactttctct tccagacgca ccagttcttc
     9841 aatctgtacc aggaaccagc ggtcaatgtt agtcaggttg aagacgccgt ccacagacag
     9901 gcccgcgcgg aaagcatcgg cgatgtacca gatacgctct gcgcctgcgt ctttcagttc
     9961 gcgacggatt ttggttaacg cttccgggtc atccaggctc actttcgggt cgaatccagt
    10021 cgcaccgact tccaggccgc gcagcgcttt ttgcagggat tcctgctgcg tacgaccaat
    10081 cgccatcact tcgccaaccg atttcatctg agtggtcaga cggtcgttag caccggcgaa
    10141 tttttcgaag ttgaagcgag gaattttagt aaccacatag tcgatggacg gctcgaagga
    10201 ggccggagta cgtccgccag tgatgtcgtt catcagttcg tcgagggtgt aacccaccgc
    10261 cagtttcgcc gccactttag caatcgggaa accggtcgct ttcgacgcca gcgccgaaga
    10321 acgggacacg cgtgggttca tttcgataac aatcaggcga ccgtttttcg ggttcaccgc
    10381 aaactggaca ttggaaccac cggtttcaac gccgatttca cgcagcaccg ccatcgaggc
    10441 gttacgcatg atttgatatt ctttgtcggt cagcgtttgg gctggcgcga cagtgatgga
    10501 gtcaccggtg tggatgccca tcgcatcgaa gttttcgata gagcagacga tgatgcagtt
    10561 gtcgttttta tcacgcacca cttccatctc gtactctttc cagccgatca gcgactcatc
    10621 aatcagcaac tctttggtcg gagagagatc cagaccgcgg gcgcaaattt cttcaaactc
    10681 ttcacggtta taagcgatac cgccgccgct accgcccatg gtaaaggatg ggcgaataat
    10741 gcacgggaag cccacgtcag cggcaaccgc cagcgcttct tccatcgtgt gtgcgatacc
    10801 ggaacgcgcg gtttccagac caattttctt catcgctacg tcgaaacgac ggcggtcttc
    10861 tgctttatca atcgcatcgg cagtggcacc aatcatggtg acgccgaact cttccaacac
    10921 gccctgacgt tccagctcca gcgcgcagtt cagcgccgtc tgaccgccca tggttggcag
    10981 caccgcgtcc gggcgctctt tttcaataat cttgcgtacc acttcccagt gaatcggctc
    11041 gatgtaggtc gcatcggcca tttccgggtc ggtcatgatg gtcgccgggt tggagttcac
    11101 cagaatgacg cggtaaccct cttcgcgcag ggctttacat gcttgcgcgc cagaatagtc
    11161 aaactcacac gcctgaccga taacaatcgg gcccgcaccc agaatcagga tactttttat
    11221 atctgtacgt tttggcatgg ctcttttact cctgattact tagcggtttt acggtactgc
    11281 tcaattaact cgataaagtg gtcgaacaac ggcgcggcgt cgtgtggacc tgggctggct
    11341 tccgggtgcc cctggaagct gaacgccggt ttatcggtgc gatgaatgcc ctgtaacgta
    11401 ccgtcgaaca gggatttatg cgtgacacgc aggtttgcag gtaatgttgc ttcatccacc
    11461 gcaaaaccgt ggttctgggc ggtgatcatc accacgtttt tctcaacatc tttaaccgga
    11521 tggttgccgc cgtggtgacc aaatttcatt ttgacagtct tcgcaccgct cgccagcgcc
    11581 agcagctgat gaccgagaca gatgccgaat accggaatat cggtttcgag gaacttctgg
    11641 atggcggtaa tggcgtaatc gcacggggcc gggtcgccag gaccgttgga gaggaagatg
    11701 ccgtctggat tcattttcag cacatcttcc gcagaagttt gcgccggaac gatggtcagg
    11761 cgacagcctc tatccaccag catccgcagg atgttgcgct tggcaccaaa atcataagcc
    11821 acgacgtgga acggcagctc atcttctttt ttcgcttctg gcaggccgcc ggtcaacgtc
    11881 cagctccctt gtgtccagct ataggcttct gcggtggtca cttcttttgc cagatccatg
    11941 ccgttcagac ctgggaacgc gcgggctttt tctaacgcca gcgccgcatc cgggttatcg
    12001 cctgcgataa tgcagccatt ctgtgcgcct ttctcgcgca gtaaacgcgt cagcttacgg
    12061 gtatcgatat cggcaatcgc cacgatgtta tggcgcttca ggtaagaaga gaggtcttcg
    12121 gtattacgga agttgctggc aatcagcggc agatcgcgaa taaccagacc ttgtgcatgt
    12181 acctgagaag attcttcatc ggcgtcatta gtgccgacat tgccaatatg gggataagta
    12241 agagtaacga tttgacgaga ataggaagga tcagtgagga tttcttgata accggtcatt
    12301 gaagtattga aaacgacttc cccaaccgcc gaacctgttg cccctatggc ccgaccgtga
    12361 aactgggttc cgtcttccag aaccaatagc gctgacttaa tcaaaacacc ctccagagaa
    12421 tattcactca ctttatttgc atattaattc acaatgatga cgtaaatcaa tgcaaatctg
    12481 cttaccgacg aatttctggc aaacggcggc attctggaga tatgggcgct aaaagtcaac
    12541 ttaatggcgg tattttttat attattttgt ggctcatgca tgttttacag gcattaagcg
    12601 acaaaaagat catataacct gttctggaaa acgct
//
LOCUS       DAPGOF010000142        12531 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.143, whole genome shotgun sequence.
ACCESSION   DAPGOF010000142 DAPGOF010000000
VERSION     DAPGOF010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.143"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(24..989)
                     /gene="glk"
                     /locus_tag="RHV13_003553"
     CDS             complement(24..989)
                     /gene="glk"
                     /locus_tag="RHV13_003553"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HDV1243053.1"
                     /translation="MTKYALVGDVGGTNTRLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            1193..2449
                     /locus_tag="RHV13_003554"
     CDS             1193..2449
                     /locus_tag="RHV13_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HDV1243054.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPASEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            2564..2890
                     /locus_tag="RHV13_003555"
     CDS             2564..2890
                     /locus_tag="RHV13_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HDV1243055.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(3031..4269)
                     /locus_tag="RHV13_003556"
     CDS             complement(3031..4269)
                     /locus_tag="RHV13_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HDV1243056.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            4605..5807
                     /gene="nupC"
                     /locus_tag="RHV13_003557"
     CDS             4605..5807
                     /gene="nupC"
                     /locus_tag="RHV13_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HDV1243057.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(5857..8046)
                     /locus_tag="RHV13_003558"
     CDS             complement(5857..8046)
                     /locus_tag="RHV13_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HDV1243058.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEVGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSIYRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(8254..8329)
                     /locus_tag="RHV13_003559"
     tRNA            complement(8254..8329)
                     /locus_tag="RHV13_003559"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8294..8296),aa:Ala,seq:ggc)
     gene            complement(8369..8444)
                     /locus_tag="RHV13_003560"
     tRNA            complement(8369..8444)
                     /locus_tag="RHV13_003560"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8409..8411),aa:Ala,seq:ggc)
     gene            8665..9024
                     /locus_tag="RHV13_003561"
     CDS             8665..9024
                     /locus_tag="RHV13_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HDV1243059.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEALALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            9026..9418
                     /locus_tag="RHV13_003562"
     CDS             9026..9418
                     /locus_tag="RHV13_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HDV1243060.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(9470..10885)
                     /gene="gltX"
                     /locus_tag="RHV13_003563"
     CDS             complement(9470..10885)
                     /gene="gltX"
                     /locus_tag="RHV13_003563"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDV1243061.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYFQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            11144..11219
                     /locus_tag="RHV13_003564"
     tRNA            11144..11219
                     /locus_tag="RHV13_003564"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11177..11179,aa:Val,seq:tac)
     gene            11264..11339
                     /locus_tag="RHV13_003565"
     tRNA            11264..11339
                     /locus_tag="RHV13_003565"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11297..11299,aa:Val,seq:tac)
     gene            11386..11461
                     /locus_tag="RHV13_003566"
     tRNA            11386..11461
                     /locus_tag="RHV13_003566"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11419..11421,aa:Val,seq:tac)
     gene            11466..11541
                     /locus_tag="RHV13_003567"
     tRNA            11466..11541
                     /locus_tag="RHV13_003567"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11499..11501,aa:Lys,seq:ttt)
     gene            complement(11650..12504)
                     /gene="xapR"
                     /locus_tag="RHV13_003568"
     CDS             complement(11650..12504)
                     /gene="xapR"
                     /locus_tag="RHV13_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001692954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HDV1243062.1"
                     /translation="MLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKELENQLGTQLF
                     IRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELGVVGTAMWGRM
                     RPVMRRFLRENPNVDVLFREKMPAMQMALLERRELDAGIWRMATEPPTGFTSLRLHES
                     AFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQVGFSPVVIREV
                     NEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKQRIPADLYIVYETQQVTPAMV
                     KLLAALTQ"
BASE COUNT         3209 a         3279 c         3015 g         3028 t
ORIGIN      
        1 cctcccgata taacaggaag gatttacaga atgtgaccta aggtctggcg taaatgtgca
       61 ccggaaccga gaagacccgg attgtcatgg acgatgagat acaccggaat atcatggaca
      121 tattctttaa agcgcccttt atcttcaaat gcggcacgga aaccagaggc tttgaagaac
      181 tcaaggaagc gcggcacgat accgcccgca ataaacacgc cgccaaatgt cccgagattg
      241 agcgccagat tgccgccaaa acggcccata atgacgcaaa acagcgacaa cgcacggcgg
      301 caatcggtgc agctgtcagc cagcgcgcgt tcggtaatat cttttggctt gagattttct
      361 ggcaggcggt tgtcagcttt cacaattgcg cgatacaaat tcaccagccc agggccagaa
      421 agcacgcgtt ccgccgaaac atggccaatt tccgcacgca atatttcgag gataatggcc
      481 tcttcttcac tattcggcgc aaaatcaacg tgaccgcctt cgcctggcaa gcttacccaa
      541 cgcttatcga catggaccag atgcgcaacc ccaagccccg ttccggcacc gtaaaccgca
      601 ataggcttac cttctaccgg ttctgcgcca ccaaactgaa tcagatgctc ttttttcagc
      661 atcgggatcg ccatcgatac agcggtaaaa tcgttaataa tttccagatg gctaaaaccg
      721 agattctttt tcatttcggc aattgagaac gcccaggtat ggttggtcat tgccacccag
      781 tctccggtaa tcgggcaagc gatggcaata cagccgtctt tcacctcgac cttatgttct
      841 tcaagataaa cgcgaatgac cgcttcgagg ctggggtaat caagccctga ataggtctta
      901 gcctgcgaga tttcaccact ggcaatatca cacagagcaa gacgtgtgtt ggtgccgccc
      961 acatcaccga ctaatgcata ctttgtcatt cttcaactgc tccgctaaag tcaaaataat
     1021 tctttctcac actgtaaata cctgggggca taacaacaac gccggaaagg caggctccct
     1081 gtaaatatcg atctgggtca cacaattact ttatcgtttc agcaccaatt gcagcgatgc
     1141 ctttttgcaa gctgggcaaa ctaagtatct gaccccgcat aaggaataga acatgctcca
     1201 tccgcgagcc agaaccatgt tgttattatc gctccccgcc gtggcaattg ggattgcgtc
     1261 cagtcttatt ctgattgtgg tgatgaaaat cgcctcggta ttacagaatt tgctctggca
     1321 acgactgccg ggaactctgg ggatagccca ggattcaccc ctctggatca tcggtgtatt
     1381 aacgctaacg ggtattgcgg tggggttggt tatccgtttc agccagggtc atgccggacc
     1441 agaccccgcc agtgaaccgc tgatcggcgc accggttccg ccctctgcgc tacctggact
     1501 tatcgtagca ttaattctcg gtcttgctgg cggcgtcagc ctggggccgg aacatccgat
     1561 catgaccgtc aatatcgccc ttgcggttgc cattggcgct cgtctgttac cgcgcgtcaa
     1621 ccgaatggag tggactattt tagcctctgc cggaaccatc ggcgcgctgt ttggtacacc
     1681 tgttgcggcg gcgttgatat tttcgcaaac cttaaatggc agtagtgaag ttccgctatg
     1741 ggatcgtctt tttgcgccgt taatggcggc agcagctggt gcacttacta ccggattatt
     1801 tttccatcct catttttcac tgcccattgc gcattacgga cagatggaaa tgaccgatat
     1861 tctcagcggt gcaattgtcg cggcgattgc catcgcagca gggatggtcg ccgtatggtg
     1921 cttaccacga ttgcacgcga tgatgcatca aatgaaaaat ccggtgctcg tgctgggtat
     1981 tggcggattt attctcggta ttctgggggt tattggtggg ccggtttcgc tgtttaaagg
     2041 actggatgag atgcagcaga tggtggcaaa tcaggctttc agcaccagcg attacttttt
     2101 gctggcggta attaaacttg ccgccctggt cgttgctgcc gccagtggct ttcgcggtgg
     2161 gcgaatcttc ccggcagtgt tcgtcggcgt ggcattaggg ttgatgctgc atgagcacgt
     2221 ccccgccgtt ccagcggcaa taaccgtttc ctgcgctatt ctcggcatcg tgctggtggt
     2281 aacacgcgat ggctggttaa gtctttttat ggcggcagtc gttgtaccca ataccacatt
     2341 gctaccgctg ctctgtatcg tcatgcttcc ggcatggctg ttattagcag gtaagccgat
     2401 gatgatggtc aatcgtccga agcaacagcc accccacgat aacgtttagc aaaaaatgct
     2461 ctcctttatg atttaagagt tatggcgatg atacggggct ttacctcccc gtaatattgc
     2521 gttaacaggc cgctgacggc ttcgatcgtg aaggagaata acaatgttca ggtcactgtt
     2581 tctggcggcc gccctgatgg catttacccc gcttgcagca aacgcaggtg aaatcaccct
     2641 actgccatca atcaaattac aaattggcga tcgcgatcat tacggtaatt actgggacgg
     2701 tggtcactgg cgagatcgtg attactggca tcgcaattat gaatggcgta aaaaccgctg
     2761 gtggcgtcat gataatggct accaccgtgg ctgggataag cgtaaagcgt atgagcgtgg
     2821 ctatcgtgaa ggctggcgcg atcgtgacga tcatcgcgga aaaggccgcg gacatgggca
     2881 ccgccattaa acacatttgc caatggagca caaggcctga tgcgacgcta gtgcgtctta
     2941 tcaggcctac aaaccggctc tttaacgcgt agggcggata aggcgtttac gccgcatccg
     3001 ccaatagtgc cagatgcgac gctcattcaa ctacaatccc agcgccgtcc ccaccaacaa
     3061 ccagatattc agcgccacga ccagcactac aatcacccag cctgtctgtt ttacgcgttt
     3121 gctgttcacc agatcgccca tcaacttgct gtcactggtg aaaatcagca gtggaaccag
     3181 cgccagggcg ataccaaaac ttaacagcac ctgactcata accagaatcc gtgtcggatc
     3241 taatcccatc agaatgacaa taaatgacgg caacatggtg actgtacgac gcacccacag
     3301 cgggatatgg aagcgaatga acccttgcat caccacctgc cccgccagtg tccccaccac
     3361 cgttgaagac agtccggcag caaccaagct taatccaaag accgttgccg cagcatggct
     3421 taacagcggt tgcagcgtca aataagcctc atcaagatcg gcaacaccag tatgaccaga
     3481 aaagtggaac gccgccgcag ctgtagccat catcgccaga ttgacaaaac cggcaatagt
     3541 catggcgata gccacatccc atttggtggc ggaataacgt tgttgacgcg aaccgccatg
     3601 taaatgctga gtgagcgaag agtgcaaata aatcacatgc ggcataatcg tcgcccctaa
     3661 cacgcctgct gccaggaaga ccgcctccga agtaggtaaa ctcgggatca ccattccttt
     3721 acccagctgc gccaggttag gctgggagaa aatcaactcg acaatgtaag ccgcggcaac
     3781 aaacaacagt aacccgccaa tcactttctc caacggtttt tgcccgcgac gttgcagcat
     3841 taaaatcagg aaagtcgcga tccccgtcag caccgcgccc tgcaacaacg aaacaccaag
     3901 aatgagttta aaaccgatcg ccgcaccaat aaattccgcc aggtcggttg ccatcgcaat
     3961 aatttctgcc tgaacccaat agaaccacac tacgggacgc ggatagtgat cgcgaatctg
     4021 ctccgccaga tttttaccgg tggcaatccc tagtttggca gagaggatct gaatcagcat
     4081 cgccatcagg ttggcccaaa cgacaaccca cagtagctga tagccgaagc tggcacccgc
     4141 ctgaatattg gtcgcaaagt taccgggatc gatataacca atcgccgcaa tgaacgcagg
     4201 tcccattaat gcgagcctca tcttgcgcgc cgcccgtccg ctgctactct caacgcgata
     4261 gttcgtcatc ttgtgcctct aaaacatagc ctttgctatg tttcatgcta tgccaaacga
     4321 gaatgattat caaattcatt tgaatgggtt gtggtgattt ctttgataga ccaggattat
     4381 gactacgaaa agattgatga cttgaatgtt atggttatgt ttaatgttag cacatttaca
     4441 taactttcag cttccataca caacatagca gaaatgtatg acagatcact atttttgaag
     4501 cttgtcacag gacgtcatta tagtgtgtgt cagatctcgt tttccttaac catgttacat
     4561 agaatgtgca cggaaattta acctgcctca tatttggagc aaatatggac cgcgtccttc
     4621 attttgtact ggcacttgcc gttgttgcga ttctcgcact gctggtaagc agcgaccgca
     4681 aaaaaattcg tatccgttat gttattcaac tgcttgttat cgaagtgtta ctggcgtggt
     4741 tcttcctgaa ctccgacgtt ggtttaggct tcgtgaaagg cttctccgaa atgttcgaaa
     4801 aactgctcgg atttgccaac gaagggacta acttcgtctt tggtagcatg aatgatcaag
     4861 gcctggcatt cttcttcctg aaagtgctgt gcccaatcgt ctttatctct gcactgatcg
     4921 gtattctcca gcacattcgc gtgttgccgg tgatcatccg cgcaattggt ttcctgctct
     4981 ccaaagtcaa cggcatgggc aaactggaat cctttaacgc cgtcagctcc ctgattctgg
     5041 gtcagtctga aaactttatt gcctataaag atatcctcgg caaaatctcc cgtaatcgta
     5101 tgtacaccat ggctgccacg gcaatgtcca ccgtgtcgat gtccatcgtt ggtgcataca
     5161 tgaccatgct ggaaccgaaa tacgtcgttg ctgcgctggt attgaacatg ttcagcacct
     5221 ttatcgtgct gtcgctgatc aacccttacc gtgttgatgc cagtgaagaa aacattcaga
     5281 tgtccaacct gcacgaaggt cagagcttct tcgaaatgct gggtgaatac attctggcag
     5341 gtttcaaagt tgccattatc gttgccgcga tgctgatcgg ctttatcgcc ctgatcgccg
     5401 cgctgaacgc actgtttgcc accgtgactg gctggtttgg ctacagcatc tccttccagg
     5461 gcattctggg ctacatcttc tatccgattg catgggtgat gggtgttcct tccagtgaag
     5521 cactgcaagt gggcagtatc atggcgacca aactggtttc caacgagttc gttgcgatga
     5581 tggatctgca gaaaattgct tccacgctct ctccgcgtgc tgaaggcatc atctctgtgt
     5641 tcctggtttc cttcgctaac ttctcttcaa tcgggattat cgcaggtgca gttaaaggcc
     5701 tgaatgaaga gcaaggtaac gtggtttctc gcttcggtct gaaactggtt tacggctcta
     5761 ccctggtgag tgtgctgtct gcgtcaatcg cagcactggt gctgtaaaac catacataaa
     5821 aaagccgggg atcattcccc ggctttttta cgcctgttaa tcagctaatg gctgcgggcg
     5881 accaatcaaa tacccttgca gatattgcac cccgagctta tgcaatagcg cctgctgctg
     5941 ttgcgtctcg acaaactccg cgaccacact caatgacttc gctttcgcca gatcggtaat
     6001 tgatctcaca atcatcgcat ccagcgtatt cgtgacaata tctttcacaa agacgccatc
     6061 aattttgatg atatcagcct gcaaacgctt taaccgttcg tagttggcat atccggtgcc
     6121 aaagtcatca atcgcaatcc ggaaaccaaa cttatgcagc tgctcgatgt tgtacatgct
     6181 gctttctgcg ttagaaaacg cctgctcctc ggtgatctca agaatgaccg cctgcgggga
     6241 gatgtgataa cgcttaaaca gacgaataat ccgcccggca atattctttt gcagcagcgt
     6301 gagcggcatt aaattgactg aaaagcgcgg tccttttttg tcgcaagggt gtgttgccag
     6361 ccacttcaac aaggattcca gcacttgcaa atcaaaacgc gcgctaaggt taaactgagc
     6421 aataaggggc agaaacttat ccggggtcat aatgccgccg tcatatttca gtcgcgcgag
     6481 gatctcatca taaccttcac cctctttgtt gcgaattggc tgggcgtaga gcagcaaatc
     6541 cccctgatct aacgccgtgc gaatggtatt cagcaatagc acctgtttag tggtctgccc
     6601 ggaaaccatc tcctctctgc tatccagcgc cagcacatgg tgatgtgcgc aggattgctc
     6661 cgccagccag cttaactgcc ccaacaaggg ttgcagggtt tcctgatttc catcaaaacg
     6721 cccccaggca gcaccatagc ccatatccag cccggtattg ttccagtgaa tttgccgact
     6781 attcaggatg ttaaccatat gctggagtcg cccttccgtt tccggcccgc tcagcactaa
     6841 cagcagttca ctacccggca attgatacaa cttttcgttt tcctgcatca acggcagcag
     6901 cgtacggtaa attgagcgga tacagtgaac gcgcatcatt aacccgtaat gacgactcat
     6961 aaactcaaga ttatcaatgc gcaggcagca aaaactcttg cccacctctt gctccggtgc
     7021 ttgttccaac gcacgaaagt tgggcaggag cgttaacgga tcggtcagcg cctgcaaatg
     7081 ccagcggcga ttaagccatt cactgcgatg ataaatgcgc accatataga gcaggcaaac
     7141 gctaaaggaa atcagcaccg cgagaataaa tgccagcgaa tattcggttt ccaccccttg
     7201 caaaaagttc tggttgtaat tcagcaggca aagcgttgaa acagcccagg tgagatttaa
     7261 aaacggatag cgaagcttac cgaccccgag ggtaaagatg atgaagaaaa cgggtaccag
     7321 gtaaccggca ataaagtcgt tttcataagg tgtgcacagc agaagtagca gcacgcttaa
     7381 cgctgccagc cagcttaagg taaatgcgcg tttctcttta cccaatgacg gagcgatatc
     7441 cctgcgccac aatatctgcg caaagtgggg acttacaatc atgcgagtga gatagtagaa
     7501 aagcatgttg taaatcagca cagcggtgaa aaggcttagc aaatcaacga ccgtgaaaat
     7561 ggcatccgca tcgccgaaaa aggtggatat ctttagcggg aaatcaaaga agctccccac
     7621 aagatacatg ctgcatttga tgccgatcgg cgtcaccaaa ccaagccaga acaggcgttg
     7681 ccagacatat cggctggtca atccatagcg ccaacgcgtc cccagctgcc agcgtaatat
     7741 tgcgcaggca cacaacacga caaacgtctg gctaccgagc atgactgcag tctgtaaaaa
     7801 agagagatga aagttccaca gattggtgca aaacatcccg cataaaacgg gaacgacacc
     7861 gcgccagcca aagataaaca agattgacag cattacgcac agtggcatcc acgccagaaa
     7921 gatataactg gaatggataa tggcaagtgg cgaaataaaa cgggaaagct gaataagtac
     7981 cacggtgagc gtaaacgcta gtgtgaatat cttgatattt tttatcaggt tatgctccac
     8041 aaacatgagt acactactct cactgatcgg ggttttcagg cacacaaata ataagtttat
     8101 tcgcatcgcg aagcaaacaa gggccagcct gttgccgatt tttcatccaa tgatgctttc
     8161 accaggcggc gctaacccgg acgtagactg tattacaaaa gcggcaaaaa gcagagacaa
     8221 aaaacccccg ctttgcagcg agggttggaa atttggtgga gctaagcggg atcgaaccgc
     8281 tgacctcttg catgccatgc aagcgctctc ccagctgagc tatagcccca cgatgcgttt
     8341 acgtaccaaa tttgctgggt gcaaaatttg gtggagctaa gcgggatcga accgctgacc
     8401 tcttgcatgc catgcaagcg ctctcccagc tgagctatag ccccgtcacg taaagcttgt
     8461 cgagttgacg ggcggcatca tatgaattcc gcccgaatgt gtcaacggca aattgcagcg
     8521 tgtagtttca atcgctgaaa aatcaggcaa atgaacaatt ttggaaaacc gctcgcattc
     8581 agtagttatt catgtcacgg tttcctgtaa agtggtgcta taaaatgaac tactaataga
     8641 cccacataca ctcagggaat tgttatgttc aaggagagga tgacgccaga tgaacttgcc
     8701 agactgaccg gctatagccg ccagaccatt aataaatggg tacgcaagga aggctggacg
     8761 acgtcaccaa aaccaggcgt ccagggtggc aaagcccgac tggttcacgt caatgaacag
     8821 gttcgtgaat atattcgtaa tgctgaacgt ccagaaggtc agggagaagc gcttgccctt
     8881 tctggtgatg cgccgcttga agtattgctg gtgacactgg cgaaagagat gacgccagtt
     8941 gaacaaaaac agtttacatc cttgcttctg cgggaaggga ttatcggatt gttacaacgc
     9001 ttagggattc gcgatagcaa ataatatgaa aagattacgc aataaaatga ccaccgaaga
     9061 actggctgaa tgcctcggtg tggctaaaca aactgttaac cgttggatca gagaaaaagg
     9121 ctggaaaacg gaaaaatttc ccggcgtgaa aggcggtcgt gccagactta ttctggtcga
     9181 tacgcaagtt tgcgagttta ttcagaacac gcctgcgttc cataacacac caatgttgat
     9241 ggaagctgaa gagagaattg ctgaatacgc tcctggagca cgcgccccgg cgtatcgaca
     9301 aatcatcaac gcgatagata atatgacaga cattgagcag gaaaaagtcg cgcaattttt
     9361 gtcacgtgaa ggaattcgca atttcctcgc ccgtctcgat attgacgaat cagcataaat
     9421 aaaaaacggc aggatattat ctcctgccgt ttatcttttt acacgctaat tactgctgat
     9481 tttcgcgttc agcaataaaa tccagcgctt tgttgatacg ctcgatactg cgggtcttac
     9541 caattgcgtg aacggtaaca tccagtgctg gagactgccc cgcaccggtt acggcgacac
     9601 gcagcggcat accaacttta cccataccca cttccagctc atccgccgtc gcctgaatag
     9661 cgtgatgaac gttttcagcg gtccagtcag taatcgcggc cagtttgtca cgaaccactt
     9721 ccagcggctg acgcgctacc ggacgcagat gttttttcgc cgcatcggca tcgaactcag
     9781 caaaatcttc gtagaaataa cggcagctct gtgccatctc tttcagcgtc ttgcagcgtt
     9841 cgcccagcag tttcaccaga tcagccagct gcgggccgtt acgggtatcg atattttcct
     9901 gctcaatgtg ccactgtaag tgagtagcaa catactccgg cggcagcgcg ttaatgtagt
     9961 gatggttcag ccacagcagc ttgtcggtgt tgaacgcact ggcagatttg ctgacggcat
    10021 tcaaagtgaa gtatttgatc atctcttcac gagtgaagat ttcctgatcg ccgtgggacc
    10081 agcccagacg caccagatag ttcagcagtg cttctggcaa ataaccgtca tcacgatact
    10141 gcattacgct gactgccccg tgacgtttgg acagtttttt accgtcatcg ccattgatca
    10201 tagaaacgtg cgcgtaaacc ggcaccggcg ctttcagggc cttaagaatg ttgatctggc
    10261 gtggcgtgtt gttgatatgg tcttcgccac ggataacgtg ggtgatttcc atatcccagt
    10321 catcgacaac cacacagaag ttataggttg gggaaccatc ggtacggcgg ataataagat
    10381 cgtccagttc ctggttgctg aactcgatcg gaccacggat ctgatcgtca aaaacaacag
    10441 aaccttcctg cgggttagca aaacgtacga cacacggctc atcatcagcg tgatgctcat
    10501 gactgtggcg gcagcgaccg tcataacgcg gcttctcacc tttcgccatt tgctcttcgc
    10561 gcagcgcttc caggcgctct ttagagcaat agcatttata agctgtgccc tcttccagca
    10621 tctgatcgat caccgcgttg tagcgatcaa aacgtttggt ctggaagtac ggaccttcat
    10681 cccactccag gctcagccag ttcatgccat ccataatggc ttcgatagct tccggcgtgg
    10741 aacgctcaag atcggtgtct tcaatacgca gcacgaactc accgccgtgg ttacgtgcaa
    10801 aaagccagga gtaaagagca gtacgcgcgc cgccaacgtg cagatagcct gttgggcttg
    10861 gcgcgaagcg agttttgatt ttcatgaaat ggccttacgt ttagaaagat gccgacaacc
    10921 ggcaaatcct ggaaaaattg agtgggcgat attctatcac tcccgcctga ttcctcaatg
    10981 tagttcgggt tgcaacctta cgcattattg ttttcgttga caaattgcgc aacacggtct
    11041 gttttgcgat catcttgctt aaatttacga cgaacgaaca atttctttag aaaatgcgtt
    11101 gactcatttt gaactctccc tataatgcga ctccacacag cgggggtgat tagctcagct
    11161 gggagagcac ctcccttaca aggagggggt cggcggttcg atcccgtcat cacccaccaa
    11221 ctactttatg tagtctccgc cgtgtagcaa gaaattgaga agtgggtgat tagctcagct
    11281 gggagagcac ctcccttaca aggagggggt cggcggttcg atcccgtcat cacccaccac
    11341 tttctcacca gctaaatttc ttgtaacaat gtgaagtacc gaagtgggtg attagctcag
    11401 ctgggagagc acctccctta caaggagggg gtcggcggtt cgatcccgtc atcacccacc
    11461 acttcgggtc gttagctcag ttggtagagc agttgacttt taatcaattg gtcgcaggtt
    11521 cgaatcctgc acgacccacc aatgtaaaaa ggcgccctaa aggcgctttt ttgctatctg
    11581 cgatttgcga aattgcctga tgcgcttcac ttagcagact actatttccg gcaattcctg
    11641 tctcctcacc tactgtgtca atgcagccaa cagcttaacc atcgcgggcg tcacctgctg
    11701 tgtttcataa acaatatata aatctgcagg gatgcgctgt ttgagcggac ggaaaatgac
    11761 acctggccag ttcatttgtg cgtagctgtc cgctatcaat gtgataccaa tgcccatact
    11821 gaccatagcg agtaccgttt gcggttcatt aacttcgcga ataacaaccg gtgaaaatcc
    11881 cacctgctgg caaactcgct gcaaaaaatc ccagtcagtg taaacgggcg gcattgtaac
    11941 aaaatactcg tcacgtagcg cttccagcgg gacggtggaa aatgatgaga gatgatgctc
    12001 ttcaggcatc gccaccagaa acgccgattc atgcaaccgt aagctggtaa aaccagtcgg
    12061 tggttctgtc gccattcgcc agatcccggc atcaagttcg cggcgttcca gcaaggccat
    12121 ttgcatcgcg ggcatctttt cgcgaaaaag aacgtcaacg ttaggatttt ccctgaggaa
    12181 tcgccgcata accgggcgca tccgtcccca cattgccgtt cccactacgc cgagttcaat
    12241 ccgccctgct tctccccgac ctatttgttc aacccgagcc aatacattat tagcattcac
    12301 cagcaatcga cgcgattctt ccatcaagat tttgcccgcg tgtgtcagta cgacgctgcg
    12361 cgaatggcga ataaaaagct gcgtgccgag ttgattttcc agttctttaa tatgaatact
    12421 gagcggaggc tgagacatat ttaaacgcgc tgctgcgcgg ccaaaatgca actcttccgc
    12481 tacggcaaga aaataacgga gcaacttaag atctgttctg tatacgcgtt c
//
LOCUS       DAPGOF010000143        12487 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.144, whole genome shotgun sequence.
ACCESSION   DAPGOF010000143 DAPGOF010000000
VERSION     DAPGOF010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12487
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.144"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            173..2314
                     /locus_tag="RHV13_003569"
     CDS             173..2314
                     /locus_tag="RHV13_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HDV1243063.1"
                     /translation="MAKFTPSFSGIKGRALFSLLFAAPMIHATDTATTKDGETITVTA
                     DANTATEATDGYQPLSTSTATLTDMPMLDIPQVVNTVSDQVLENQNATTLDEALYNVS
                     NVVQTNTLGGTQDAFVRRGFGANRDGSIMTNGLRTVLPRSFNAATERVEVLKGPASTL
                     YGILDPGGLINVVTKRPEKTFHGSVSATSSSFGGGTGQLDITGPIEGTQLAYRLTGEV
                     QDEDYWRNFGKERSTFIAPSLTWFGDNATVTMLYSHRDYKTPFDRGTIFDLTTKQPVN
                     VDRKIRFDEPFNITDGQSDLAQLNAEYHLNSQWTARFDYSYSQDKYSDNQARVTAYDA
                     TTGTLTRRVDATQGSTQRMHSTRADLQGNVDIAGFYNEILGGVSYEYYDLLRTDMIRC
                     KNAKDFNIYNPVYGNTSKCTTVSASDSDQTIKQESYSAYAQDALYLTDNWIAVAGIRY
                     QYYTQYAGKGRPFNVNTDSRDEQWTPKLGLVYKLTPSVSLFANYSQTFMPQSSIASYI
                     GDLPPESSNAYEVGAKFELFDGITADIALFDIHKRNVLYTESIGDETIAKTAGRVRSR
                     GVEVDLAGALTENINIIASYGYTDAKVLEDPDYAGKPLPNVPRHTGSLFLTYDIHNMP
                     GNNTLTFGGGGHGVSSRSATNGADYYLPGYFVADAFAAYKMKLQYPVTLQLNVKNLFD
                     KTYYTSSIATNNLGNQIGDPREVQFTVKMEF"
     gene            complement(2378..4270)
                     /gene="parE"
                     /locus_tag="RHV13_003570"
     CDS             complement(2378..4270)
                     /gene="parE"
                     /locus_tag="RHV13_003570"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDV1243064.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDNDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(4299..4880)
                     /gene="yqiA"
                     /locus_tag="RHV13_003571"
     CDS             complement(4299..4880)
                     /gene="yqiA"
                     /locus_tag="RHV13_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HDV1243065.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(4880..5707)
                     /gene="cpdA"
                     /locus_tag="RHV13_003572"
     CDS             complement(4880..5707)
                     /gene="cpdA"
                     /locus_tag="RHV13_003572"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HDV1243066.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(5732..6154)
                     /locus_tag="RHV13_003573"
     CDS             complement(5732..6154)
                     /locus_tag="RHV13_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HDV1243067.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(6155..6784)
                     /gene="nudF"
                     /locus_tag="RHV13_003574"
     CDS             complement(6155..6784)
                     /gene="nudF"
                     /locus_tag="RHV13_003574"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HDV1243068.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            6989..8470
                     /gene="tolC"
                     /locus_tag="RHV13_003575"
     CDS             6989..8470
                     /gene="tolC"
                     /locus_tag="RHV13_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HDV1243069.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            8618..9289
                     /locus_tag="RHV13_003576"
     CDS             8618..9289
                     /locus_tag="RHV13_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HDV1243070.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            9295..10455
                     /locus_tag="RHV13_003577"
     CDS             9295..10455
                     /locus_tag="RHV13_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HDV1243071.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVDKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(10493..11281)
                     /gene="ygiD"
                     /locus_tag="RHV13_003578"
     CDS             complement(10493..11281)
                     /gene="ygiD"
                     /locus_tag="RHV13_003578"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HDV1243072.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            11424..12197
                     /gene="zupT"
                     /locus_tag="RHV13_003579"
     CDS             11424..12197
                     /gene="zupT"
                     /locus_tag="RHV13_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HDV1243073.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWFILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(12255..12425)
                     /locus_tag="RHV13_003580"
     CDS             complement(12255..12425)
                     /locus_tag="RHV13_003580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243074.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
BASE COUNT         3135 a         3321 c         3063 g         2968 t
ORIGIN      
        1 aaacacatgt agcaattttt tgttatttct catttactac tacattatcg catggttgaa
       61 tgattctcaa ttacatatcc gatggtgaaa taacaaacgg atagtgctta gtgagcaaca
      121 aaaaacaatt ttcactcacg acaatccgtg agtttttggg ataacagact aaatggctaa
      181 gttcacacct tcattctcag gaatcaaagg tcgggcgctc ttttcactgc tctttgcagc
      241 accaatgatt catgcaaccg acactgcaac gaccaaagat ggcgaaacaa tcactgttac
      301 agcggatgca aataccgcaa ctgaggcgac cgatggttat caacctctga gcacttccac
      361 ggcgacatta accgatatgc cgatgctgga tatcccgcag gtggtcaata cggttagcga
      421 tcaggttctg gaaaatcaga atgcaacgac gctggatgaa gcgctttata acgtcagtaa
      481 cgtggtacag accaatacat taggcggaac tcaggacgcc tttgtacgtc gtgggtttgg
      541 tgctaaccgg gatggctcca tcatgaccaa cggcctgcga actgtacttc ctcgcagttt
      601 caacgccgcc acagaacgtg tggaagtgct aaaaggtccg gcctccacgc tgtatggcat
      661 tctcgatcct ggtggattga ttaacgtcgt gaccaagcgc ccggaaaaaa cattccatgg
      721 ttctgtctca gccacgtcct ccagttttgg cggaggcact gggcaacttg atatcacagg
      781 tcccattgaa ggcactcagc tggcataccg cctgacgggg gaagtgcagg atgaagatta
      841 ctggcgaaac ttcggtaaag agcgcagtac atttattgcc ccgtcactca cctggtttgg
      901 tgataatgca acagtaacca tgctctattc ccatcgggac tataaaactc cgttcgatcg
      961 tggaacgatt ttcgacctta cgacgaaaca gcccgtaaac gttgatcgaa aaatacgttt
     1021 tgacgaaccg tttaatatta cagatggtca gtccgatctg gcgcaactca acgcagaata
     1081 tcatctcaat agccagtgga cagcgcgctt tgattacagc tacagccagg ataaatacag
     1141 cgacaatcag gctcgcgtta ccgcgtatga tgcaacgaca ggaacgctga cacggcgtgt
     1201 tgatgcaact cagggatcta cccagcgtat gcattctact cgtgcggatc tgcaagggaa
     1261 tgttgatatt gctgggttct ataatgagat tctgggtggg gtgtcatatg aatattatga
     1321 tcttctgcgc acagatatga ttcgctgtaa aaacgctaaa gatttcaata tctacaaccc
     1381 tgtttatggc aataccagca aatgcacaac ggtttcggcg tcggacagcg atcagacgat
     1441 caaacaggag agctactcag cttatgcaca ggacgcgctc tatctgaccg ataactggat
     1501 tgccgtcgcc gggatccgct atcagtatta cacgcagtat gcgggtaaag gccgtccttt
     1561 taatgtcaat actgacagcc gcgatgaaca atggacgccc aaactggggt tagtctacaa
     1621 actgacgcca tcggtatcct tatttgctaa ttattcgcaa acatttatgc cgcaatcgtc
     1681 aattgccagc tacatcggag atcttccacc ggaatcatct aatgcttacg aagtcggggc
     1741 aaaattcgag ctgttcgatg gtataaccgc agatattgcg ctgtttgata tccataaacg
     1801 taacgtgttg tataccgaaa gtattggtga tgaaaccatc gccaaaacgg caggccgcgt
     1861 tcgttcaaga ggggtagaag tcgaccttgc gggagcatta actgaaaaca ttaatatcat
     1921 tgccagctac ggctataccg atgctaaggt tctggaagat cctgattatg cagggaaacc
     1981 attgccgaat gttcctcgtc ataccggttc gctattcctg acctatgaca ttcataacat
     2041 gccaggcaat aacacactga cgtttggcgg tggtggacat ggcgtaagca gtcgttcggc
     2101 aaccaatggg gctgactatt atctgccagg ctatttcgtt gccgatgcct tcgccgcata
     2161 caaaatgaaa ttgcagtatc cggtcacact gcaattaaac gtcaaaaacc tgtttgataa
     2221 aacgtattac acctcttcca tcgccacaaa taatctgggg aatcagattg gcgatccgcg
     2281 tgaagtgcaa ttcacggtga aaatggaatt ttgatcaaaa aagcccggta tacacttgta
     2341 taccgggcta tgttttattc ttctggcggg aggattatca gacctcaatc tccgccatgt
     2401 cgcctttctc ttgcaaccag ttgcggcgat cttccgagcg tttcttcgcc agcagcatat
     2461 ccatcatcgc gtcagtacgc tgatcgtctt catcatcgat agtcagctgc accagacggc
     2521 gagtgttcgg atcaagcgtg gtttcgcgca attgcatcgg gttcatttcc cccagacctt
     2581 taaaacgctg gacgttcggc ttgcctttct tgcgttttaa ttgctcaagt acgccctctt
     2641 tctcttcttc cgtcagcgcg taataaacct ctttcccgag atcaatacgg tagagcggtg
     2701 gcagtgcgac gtaaacgtga ccgtgtttca ccaacgcgcg gaaatgtttt acaaacaaag
     2761 cgcagagcag cgtggcaatg tgcagaccat cagagtccgc atctgcgagg atacagattt
     2821 tgccataacg aagctggctc agatcgtcgt tgtctggatc gataccgatc gctaccgaaa
     2881 tatcgtgcac ttcctgcgaa gccagcactt cgtcggaaga gacttcccag gtgttaagga
     2941 tcttaccttt cagtggcatg atcgcctgat attcgcgatc gcgcgcctgc ttggcagatc
     3001 cgcctgcgga gtcaccttcc acgaggaaca gttcggtacg gttaaggtcc tgcgcggtac
     3061 aatcagccag tttgccaggc agcgccgggc cgctggtcag ctttttacgc accacttttt
     3121 tagccgcacg catacgacgc tgggcgctgg aaatcgccat ctctgccagc aactccgccg
     3181 cctgaacgtt ctggttcagc cacaggatga aggcatcttt caccacgccc gaaacgaatg
     3241 ccgcgcactg acgcgaagag agacgctctt tggtctgccc ggcaaactgc ggatcctgca
     3301 tttttactga cagcacatag gcgcagcgat cccagatatc ttccgccgac aactttacac
     3361 cgcgcggcag aatgttgcgg tattcacaga actcacgcat cgcgtccaac aggccctgac
     3421 gcagaccgtt aacgtgcgta ccgccctgca tcgttgggat aaggttgacg tagctttcgg
     3481 tcagcagttc accgccttcc ggcagccaca gtagcgccca gtccacagct tcagtatcac
     3541 cagcgaaatt accgataaac ggtttttccg gcagcgtcgg cagaccattt accgcttccg
     3601 ccaggtaatc attcagaccg tcctgatagc accagcgttg ttcggtattg ttgatctcat
     3661 ctttaaaagt gatctcaacg ccagggcaca ataccgcttt ggctttcagc acatgtgtca
     3721 ggcgtgaaac agaaaaacgc gggctgtcaa agaaggtttc atccggccag aagtgcacgc
     3781 tggtaccagt attgcgttta ccgcaagtgc cgacaacctg taaatcctgc accttttcgc
     3841 cattttcaaa ggcgatgtta taaacctgac catcgcggcg cacgttaact tctacgcgct
     3901 tcgacagggc gttaaccacc gaaatcccca cgccatgcag gccgccagag aactggtaat
     3961 ttttgttaga gaatttaccg cctgcatgca gacggcaaag aatcagttca accgccggta
     4021 caccctcttc cgggtgaata tccaccggca tcccgcgccc atcgtcaata acttctaacg
     4081 actggtcagc atgtaaaata acgtccacgc gttttgcgtg acccgccagt gcttcatcca
     4141 cactgttatc aatgacttct tgccccaaat ggttagggcg agtggtatcg gtatacatcc
     4201 ccggacggcg gcgaaccggc tcaagcccgg tgagtacctc aatggcatca gcgttataag
     4261 tttgcgtcat ggtttaagtt agtaattcga gttgatcgtc agagatggtg cagaccaaga
     4321 aaatcgacga tcgggttgaa ataatcttcg aagcccgtga atgcgtggtt gccgccttct
     4381 atgacagtct ggcggcagga agcgtagtac gccaccgcct ggcggtaatc cagcacttca
     4441 tctcccgtct gttgcagcag ccagatcaaa tccggcgctt ccagcgggtc aatctgcatg
     4501 actttaagat cgtaaatatg gcgtgactct agcacatatt gctgcccggt gtaggggttc
     4561 tcgttctgac cgagatagtc cgtcagcagt tcaaacgggc gcaccgccgg gtttaccacc
     4621 actgcgggca gcataaaaca ttgtgacaac caggtggcgt aatatccccc cagtgatgaa
     4681 ccgacaatac ccagcgaatc gccgccatgt tccaggacaa tggattccag cagctctgcc
     4741 gcgtcggaag gatacggcgg caactgcgga atgatcatct caacgtcagg gtgatgttcc
     4801 gccagccagt tttttaacaa gctcgctttt gcagagcgcg gcgagctgtt gaaaccgtgt
     4861 aaataaagaa gcgtagacat cagtagcctt ctgaagcggt atcaggttgg aaacgtgtgt
     4921 ccgccaggcg atgcacctcg gtggtcagcg tgccatcagc atgtaactcg agagtacgcc
     4981 agccgggcgc gatagtatcc agcgtaaagt tggaacagtg cggcttaaac tgtacacagg
     5041 tcgacggcgt tgccagcagg cggcgaccgt tccagtcgag atccagctcc tgatgaatat
     5101 gaccgcacag caagtatttg acgtgcggaa acttcgccag cacggtatcc agttcgcccg
     5161 cgttacgcaa actgtgttga tcgagccaac tacaacccgc aggtagcgga tgatgatgca
     5221 gcagcagcaa cgtatggcgt tctggcgcat cggccagttt acgttccagc cactcaagct
     5281 gaaactcgct cagctcaccg tgcggcacgc caaacacctg gctatccagc aacaggattt
     5341 gccattgctc accaataaac acccgcttcg ccggggagat acccgcatcc tgtaacgcgc
     5401 tgtacatcgc gggctggaaa tcgtggttgc caggcagcca gacgcagggc gcacgaaaac
     5461 ttgcgatgcc ttcagcgaaa tgctgatagg ccgcagagga ttgatcctgc gctaaatcac
     5521 ctgtcgcgac aatcaggtcg aattcgtgct ggtgtggacg aatcgcctcc agcaccgcct
     5581 ggtagctttc ccaggtattt acccctaaca gggcttcgtg cttttgtgca aacaggtgag
     5641 tatcggtaat ttgtaaaatc ctgactctgg cctcaccagc cagaggaagg gttaacaggc
     5701 tttccaaatg gtgtccttag gtttcacgac gctagtaaac cggaatcgcc atcgctccat
     5761 gtgctaaaca gtatcgcaac cagtccgcta aaaactggtt aatttgatgc ttttcgtcgc
     5821 gttgatgcaa ctttttatta ggataatcat accgcgcttt gaagcgaaaa atctgctggc
     5881 ttgaacacac ttcagccacc atcgcgtcat gatacagacg caccgtcatt gacggaaggc
     5941 tccagtaact gatcgcgggc gcagtctgtt ctattgtcac cagggtagtg tatcgggtcg
     6001 attccacaat cgtcagccga tattgtgcgt ttgccacctg atagcttaca gtttcgccgg
     6061 gtgcgtcatt gcgcggtaac aaacggcgca attgtgaaaa gttcatctcg cacaggcgca
     6121 tcatttcagg aaagtcaggt gtgtaacgct tcatttatgc ccactcattt tttaacgctt
     6181 gatgatgcag ctgcagccat tgcaaagcga tgaccgacgc tgcgttgtcg attttcccct
     6241 cttctaccca ctggtatgcc tgttcccggc ttaccacatg aacgcgaata tcttcgtttt
     6301 catcagccag accgtgaata ccgcttgcgg tcgtggcgtc cacttcgccc accataattg
     6361 acgaacgctc actggtgccc cccggacttg ccaggaaact taacaccggt ttggtccgtt
     6421 tgactatcag ccccgcctct tcaatcgctt cgcgacgggc aacatcttcc acactttcac
     6481 cctcttcaat catcccggca accatctcca gtagccaggg ggtttcgcta gtgtcgtacg
     6541 cggcaattcg aatctgctca atcagcacaa cttcatcacg cactgggtca aagggtagca
     6601 agactgcggc gtgaccgcgc tcaaaaattt cccgccgtac ctcatgactc atttgcccgt
     6661 tgaatagacg atgacgaaat ctataaagat ctaatgaaaa aaagccgcga taaagtgttt
     6721 ctcgtgcaat aatttctaca tcgtttttgc caaatgtaac gggcaggttg tctggcttaa
     6781 gcattgttaa tgtcctggca ctaatagtga attaaatgtg aatttcagcg acgtttgact
     6841 gccgtttgag cagtcatgtg ttaaattgag gcacattaac gccctatggc acgtaacgcc
     6901 aaccttttgc ggtagcggct tctgctagaa tccgcaataa ttttacagtt tgatcgcgct
     6961 aaatactgct tcaccacaag gaatgcaaat gaagaaattg ctccccattc ttatcggcct
     7021 gagcctttct gggttcagtt cgttgagcca ggccgagaac ctgatgcaag tttatcagca
     7081 agcacgcctt agtaacccgg aattgcgtaa gtctgccgcc gatcgtgatg ctgcctttga
     7141 aaaaattaat gaagcgcgca gtccattact gccacagcta ggtttaggtg cagattacac
     7201 ctatagcaac ggctaccgcg acgcgaacgg catcaactct aacgcgacca gtgcgtccct
     7261 gcagttaact caatccattt ttgatatgtc gaaatggcgt gcgttaacgc tgcaggaaaa
     7321 agcagcaggg attcaggacg tcacatatca gaccgatcag caaaccttga tcctcaacac
     7381 cgcgaccgct tatttcaacg tgttgaatgc tattgacgtt ctttcctata cacaggcaca
     7441 aaaagaagcg atctaccgtc aattagatca aaccacccaa cgttttaacg tgggcctggt
     7501 agcgatcacc gacgtgcaga acgcccgcgc gcagtacgat accgtgctgg cgaacgaagt
     7561 gaccgcacgt aataaccttg ataacgcggt agagcagctg cgccagatca ccggtaacta
     7621 ctatccggaa ctggcggcgc tgaatgtcga aaactttaaa accgacaaac cacagccggt
     7681 taacgcgctg ctgaaagaag ccgaaaaacg caacctgtcg ctgttacagg cacgcttgag
     7741 ccaggacctg gcgcgcgagc aaattcgcca ggcgcaggat ggtcacttac cgacgctgga
     7801 tttaacggct tctaccggga tttctgacac ctcttatagc ggttcgaaaa cccgtggtgc
     7861 cgctggtacc cagtatgacg acagcaatat gggccagaac aaagtgggcc tgagcttctc
     7921 gctgccgatt tatcagggcg gaatggttaa ctcgcaggtg aaacaggccc agtacaactt
     7981 tgttggtgcc agcgagcaac tggaaagcgc gcatcgtagc gtcgtgcaaa ccgtacgttc
     8041 ctccttcaac aacattaatg cttctatcag tagcattaac gcctacaaac aagccgtagt
     8101 ttccgctcaa agctcattag acgcgatgga agcgggctac tcggtcggta cgcgtaccat
     8161 tgttgatgtg ttggatgcga ccaccacgct gtacaacgct aagcaagagc tggcgaatgc
     8221 gcgttataac tacctgatta atcagcttaa tattaagtca gccctgggta cgttgaacga
     8281 gcaggatctg ctggcactga acaatgcgct gagcaaaccg gtttccacta atccggaaaa
     8341 cgttgccccg caaacgccgg aacagaatgc tattgctgat ggttatgcgc ctgatagccc
     8401 ggcacccgtc gttcagcaaa catccgcacg cactaccacc agtaacggtc ataacccttt
     8461 ccgtaactga tgacgacgac ggggcttcgg ccccgtctga acgtaagaca acgtaaagat
     8521 acgggttatc tgccgcattc ttcccccttc tcgcttcaat ttcgaccagc catcctctat
     8581 tctgatgggt atttaccact ggtcccggaa gacaaaaatg aaacggacaa aatccatacg
     8641 ccacgcatcg ttccgcaaaa actggagcgc acgccatctg acaccagtcg ctctcgcggt
     8701 tgccactgtt tttatgctgg ctggctgtga aaagagtgat gaaacagtgt ctctctatca
     8761 aaatgctgac gactgttcag ctgcaaaccc aggcaaaagc gccgaatgta ccaccgcgta
     8821 caacaatgcg ctgaaagaag ccgaacgtac tgcgccgaaa tacgccaccc gtgaagactg
     8881 tgttgctgaa tttggtgagg gtcagtgcca gcaagcacca gcccaggctg gcatggcacc
     8941 agaaaaccag gcgcaggctc agcaatccag cgggagtttc tggatgccgc tgatggccgg
     9001 ttacatgatg gggcgtctga tgggcggcgg cgcgggattt gcacagcagc cgctgttctc
     9061 ctcgaaaaac ccagccagtc cggcttacgg taaatatacc gacgcgacgg gtaaaaacta
     9121 tggcgcagcc cagccaggcc gcaccatgac cgtaccgaag acggcaatgg caccaaaacc
     9181 ggcgaccacc actaccgtta cccgtggcgg ttttggtgaa tctgttgcca aacaaagcac
     9241 tatgcagcgt agcgcaaccg gtacctcttc tcgttcaatg ggtggctgat accgatggaa
     9301 agagtcagta ttaccgagcg cccggactgg cgtgagaaag cccacgaata cggtttcaat
     9361 tttcacacca tgtacggcga gccgtactgg tgtgaagatg cttactacaa gttgaccctc
     9421 gcccaggttg aaaagctgga agaagtcacc gccgaactgc accagatgtg cctgaaagtg
     9481 gtggataaag tgatcgccag cgatgagctg atgaccaaat tccgcattcc aaaacacacc
     9541 tggagttttg tgcgccagtc atggctgacg caccagccat cgctttattc gcgtcttgat
     9601 ctggcgtggg atggcactgg cgaacctaaa cttctggaaa ataacgccga tacgccaacg
     9661 tcactatacg aggcggcgtt ctttcagtgg atctggctgg aagatcagct taacgccggt
     9721 aacttgccgg agggcagcga ccagtttaac agtctgcaag aaaaactgat cgatcgcttc
     9781 gttgagctgc gtgaacagta tggcttccag ttgctgcatc tcacctgctg tcgcgacacg
     9841 gtggaagatc gcggaaccat tcagtatttg caggactgcg caacggaagc tgaaattgct
     9901 actgagttcc tctacatcga tgatatcggg ttaggtgaaa aaggtcagtt cacggattta
     9961 caggatcagg taatttccaa cctgttcaag ctgtatccgt gggaatttat gttgcgtgag
    10021 atgttttcca ccaagctgga ggatgcaggc gtacgctggc tggaaccggc gtggaagagc
    10081 attatctcca acaaggcact tctaccgcta ctgtgggaga tgttcccgaa tcacccgaac
    10141 ctgctgcccg cttattttgc ggaagatgat catccgcaaa tggaaaaata tgtggttaaa
    10201 ccgatcttct cccgtgaagg cgcaaacgtg tcgatcattg aaaacggcaa aaccattgaa
    10261 gcagcggaag gtccgtatgg cgaagaaggg atgattgttc agcaattcca cccgttaccg
    10321 aaattcggcg acagctatat gctgattggt agctggctgg tgaacgatca acccgccgga
    10381 attggcattc gtgaagaccg tgcattgatc acccaggata tgtctcggtt ttatccacat
    10441 atttttgttg agtaatcaat gatgccggat ggcattcgcc atccggtaat tgttaaccta
    10501 tctgcaccga cagcatactt aaactgccca tttctatacc atcgaccgga atggtaattg
    10561 gctcctgccc atcccacgca cctaagacat acagcaacgg cagatagtgc tctggcgttg
    10621 ggttcgataa cgcgccacct tcatggtcga gataattcac cagaggatgc tgctctaccg
    10681 ccccttgcca cgtcagattc gctttcacat actcattaaa cgacgtcgcc cacggatacg
    10741 gtgaactatc accgtgccac ttcactgtgc gcaggttatg caccacgtta ccgctggcga
    10801 ccaacattat tccttcatca cgcagcgccg cgagtttgcg ccccatttcg aaatgccagg
    10861 cggcaggttt gctactgtcg atactcaact gcaccatcgg gatatccgcg tcaggataca
    10921 tcttaatcag cacgccccag gaaccgtggt caaagcccca agcttcttta tccagcgtca
    10981 ccgggatcgg cgctaacagc tcaaccagac gctgtgccag cgcaggcgaa cccggagcag
    11041 gataatgcgt atcgtacagc gcctgcggga agccaccaaa gtcatgaatc gtgggcggcg
    11101 tctccatcgc ggtcactcct gttccacggg taaaccagtg agccgaaacc accacaatcg
    11161 cttgcgggcg tggcaatgtc atccccaact tctgccagct gcgggtatac aaattatctt
    11221 ccagcacgtt catcggacta ccgtgaccta aaaacaatgc tggcatacgt gttgaagaca
    11281 tgatgatatc cttaactaaa ggtgtcattt tgatatcctc acaatacgct tgttcggcgg
    11341 agtaagaacc cggataacaa tgatgatgat catcagttat tttgacgatc tgcctgaagg
    11401 tgaagattta taaggagttg tcgatgtcag tacctctcat tctgaccata ctggcggggg
    11461 cagccacgtt tattggcgcg tttctcggcg ttctcgggca aaaaccctcg aaccgcttac
    11521 tggcgttttc gctaggtttt gcggcgggga tcatgttgct catctcatta atggaaatgc
    11581 ttcctgccgc actagcagct gaaggaatgt cgcctgtgtt gggttatgga atgtttatct
    11641 tcggtctgct tggctatttt ggcctggacc gcatgttgcc acatgcgcat ccgcaggatt
    11701 taatgcaaaa atcggtgcag ccgttgccaa aatcgatcaa gcgcacagcc attctgctca
    11761 ctctcggcat cagtctgcat aacttcccgg aagggattgc cacctttgtc acggcgagca
    11821 gcaacctgga gctgggattt ggcatcgcac tggccgtcgc gttgcacaat attcctgaag
    11881 gtctggcggt tgcaggcccg gtttatgcag caacgggttc taaacgcacg gcaattctgt
    11941 gggcggggat ttctggattg gcagaaattc ttggtggtgt gctggcgtgg ttcatcctcg
    12001 gtagcatgat ttccccggtg gtcatggcgg caatcatggc ggcggttgca ggaattatgg
    12061 tggcgctctc ggttgatgaa ttaatgccgc tcgccaaaga gattgaccct aataataacc
    12121 ccagctatgg cgtactatgt ggaatgtcag tgatgggatt cagtttagtg ctgctacaaa
    12181 cggcgggaat tggttaaaaa caacatcaga agttgtccag cgcatggaca acttctgcca
    12241 gcttcacgaa agccttattc taccttttcg gctgtctctt ctctcgtact gtttaagtat
    12301 ttgttccgcg cttcaagcag tgcgtctctg tcctggcgaa acgcccgaca ataacgtttc
    12361 aaatgcagga aataacccac cacaacaaga gcaatcaata caatccagta ccaggcgata
    12421 aacatccttt tacccttaat atttaacaat actaaacatt tagcgcataa atttcacata
    12481 tcctttt
//
LOCUS       DAPGOF010000144        12339 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.145, whole genome shotgun sequence.
ACCESSION   DAPGOF010000144 DAPGOF010000000
VERSION     DAPGOF010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.145"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(27..308)
                     /locus_tag="RHV13_003581"
     CDS             complement(27..308)
                     /locus_tag="RHV13_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HDV1243075.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLTINGRISPPTCSMAMV
                     NNQPQQHCGQLTYNVDTRHQFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(570..1679)
                     /gene="apbC"
                     /locus_tag="RHV13_003582"
     CDS             complement(570..1679)
                     /gene="apbC"
                     /locus_tag="RHV13_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HDV1243076.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1811..3844
                     /gene="metG"
                     /locus_tag="RHV13_003583"
     CDS             1811..3844
                     /gene="metG"
                     /locus_tag="RHV13_003583"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDV1243077.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     VLIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3985..7779
                     /locus_tag="RHV13_003584"
     CDS             3985..7779
                     /locus_tag="RHV13_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR and DUF4132 domain-containing protein"
                     /protein_id="HDV1243078.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTQGQSQVKNFAD
                     VAAAEKAKLKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAADKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSTCSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQIDIKWDYANNVIIILPSGIAPDYLEQYSRFELR
                     LRKHLSLAEESLWQKCAQKLIAAIPHIPEWRQPLIALLLPEKPEIAHEIAQRLLGQKK
                     LPSLEWLKIVATDEHILASLEKYHEPYAIFDDYYCGAIWSATVLQEQGVVALPRFAPY
                     AASDYCADVLRHINHPFALTLLIRVAGQTKRCHDRMTKAIAAFPHAAMAALTELLGQK
                     EENSWRIMLMTMLISQPALAEQVIPWLSTPAVAVLKSCQQQLTQPSNHASADLLPAVV
                     VSPPWLSKKKKSPIPVLDLAPLGIEPICYLTEEISNQLLAKYIWYSKHITVSHEESTT
                     NLLARMGFQRRIAGTYIKAPEAVVEAWLNEDYSTLLSEFKVFHSPTGHYWQLGILTTL
                     PLEKAVKAWNALTLSPHTDTEYSMLHFGLKGLPGLVNSLARYPQEALPITNYFAASEL
                     APAVARAFNKLKTLRQDARSWLLKYPEHAITGLLPSALGKAGEAQDNARAALRMLTEN
                     GHQPLLQEIARRYNQPEVTDAVNALLALDPLDNHPTKIPTLPAFYQPSLWTRPVLKAN
                     AQSLPDNALLHLGEMLRFPQEEALYPGLLQVKDACTADSLAEFAWDLFTAWQTAGAPS
                     KESWAFTALGVLGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNG
                     IAQKLKFKALQERAKEKIADIAESRELTVAELEDRLAPDLSLDDNGSLLLDFGPRQFT
                     VSFDETLKPFVRDASGSRLKDLPKPNKSDDESQANDAVNRYKLLKKDARTVAAQQVAR
                     LESAMCLRRRWSPENFQLFLVEHPLVRHLTRRLIWGVYSTENQLLTCFRVAEDNSYST
                     ADDDLFTLPEGDISIGIPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSYALTEAE
                     RNASELTRWAGRKCPSGRVMGLANKGWIKGEPQDGGWIGWMIKPLGRWSLIMEIDEGF
                     AVGMSPAELSAEQLLSKLWLWEGKAESYGWGSNSTQEAQFSVLDAITASELINDIEAL
                     FE"
     gene            7789..11421
                     /locus_tag="RHV13_003585"
     CDS             7789..11421
                     /locus_tag="RHV13_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HDV1243079.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVPVITESIIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSIPARTMAALACIAQ
                     NDSQQLLDEIVQQEGLEYATDVVMARQCIARRYESDSLVVTLQYQDEDYGYGYGSATY
                     NDFDLRLRKHLSLAEESCWQRCADKLIAALPGIPKIRRPFIALILPEKPEIANELASL
                     ESSRSSLHSKEWLKVVATDNTAVKKLERYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAVVPRLAIYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPGYPIIEDKKLPAQQKARDEYWRTLLQTLMASQPQLAAE
                     VMPWLSTQARAVLKSYLSAPPKLVIDGTDNSNLPEILVSPPWRSKKKMTAPRLDLAPL
                     ELTPQVYWQPGERERLAATEPARYFSTESLAERMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPEAQLAEQALRSGHIEALINIWEND
                     NYSRYRAEKSVWNLYLLAQLPREMALTFWLRINEKKHLFAGEDYFLSILGLDALPGLM
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAVQRDLARQWILQWPEHTATALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTVANRWQRADVWPALEQLLKQGPMDIYPA
                     RIPKAPDFWHPAMWSRPRLITNNQPVTDDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRHFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHSLFVNHPFTRLVTQGLIWG
                     VYPANEPRRLLNAFRVAAEGEFCNAQDEPIDLPTDALIGIAHPLEMTAEMRSEFAQLF
                     ADYEIMPPFRQLSRRTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            11482..11802
                     /locus_tag="RHV13_003586"
     CDS             11482..11802
                     /locus_tag="RHV13_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243080.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSCRLEIHKCSELFTGSVYRLER
                     FRLHPTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFIQ
                     QIEK"
BASE COUNT         3231 a         3147 c         3020 g         2941 t
ORIGIN      
        1 aatgcaatta aaatcaggtt gccaaattaa tcgtagcgat ttaacacgat tcgacgtttg
       61 ctatcactac ctgcaacaac cacttctgtc gttacacctt tgaccggcga agaaaactga
      121 tgacgggtat caacattata cgttaactga ccacaatgct gttgaggttg attattaacc
      181 atagccatac tgcatgttgg gggactgatc ctgccgttaa tcgttaatgt ggcagtctct
      241 gaagagaacg cgggagaagc caatccatag gcaagaaaaa taattcctga cagccaatac
      301 ttattcattg aacgttatcc ctgtagtaaa ggttatgcct ggatagaatg agtgcataac
      361 aaactatagc tgtacatcca ctacacagca cgaaggatga taatgaagca ttgcctgtac
      421 gatcaatcga ctttgtagag tttcggacga aggtccgcag aatattcgca gtattaaata
      481 agtgttcatt aatgcggcaa aaacaaatac accgcatcaa tgtaacatct ctgcggtgta
      541 tgaagaataa ccagaatggt taataggcgt tagaccgcgc ggaaggaaat ctcgcctgga
      601 atgacttcac cctgccagta gagctgagct gcaacgcggt cagccagctg gcggtagata
      661 gcggtaaatt cgctctccgg acggctgata accgttggcg tgcctttatc gagatcctca
      721 cgtaaggaga tgtgtaacgg catctgcccc agcagctgtg tatggtattt ttcagccagt
      781 ttctctgcac caccggtacc gaaaatcggt tcatggtgac cacagttact gcaaatatgc
      841 acactcatgt tctcgacaat accgagcacc ggaacctcga ctttctcgaa catcacaatg
      901 ccttttttcg catcgatcaa cgcgatgtcc tgcggggtgg tgaccacgac tgcaccagtt
      961 actggaatgt tttgcgccag cgtcaattga atgtcaccgg tgcctggcgg catatcaagc
     1021 accagatagt cgagatccgg ccacaaggtt tcctgcaaca tctgcattaa cgccttgctc
     1081 gccatcggtc cacgccacac catcgcattg tcgtcagtga ccagataacc aattgaattg
     1141 gttgccaggc catgagacat aatcggtgcc atatgggtac cgtccggcga agttggacgc
     1201 tgattttctg cgcccagcat ggtgggaatt gatgggccgt agatatcggc gtccagaatg
     1261 ccgactttcg ccccttccgc cgccagtgcc agcgccaggt ttaccgccgt ggaggattta
     1321 cccacgccgc ctttaccgga actgaccgca atgatgttct tcacgccatt aatacccggc
     1381 tgattcttca cgcgttttaa ggtggcgata ttgtgcgaca gtttccagtc gatagctttc
     1441 gccccggtga tacgcagcag ttcggcgcta cattgctctt ttaactcttc aaacgcgcta
     1501 tgccagacga atggcataac cagttccaca tgcaacgtgt cgtccatcca ggcgacatgg
     1561 tgcaacgctt tcagcgtggt gaggttatgc ttcagggttg ggtgctgaaa attggccagt
     1621 gtcccggcga ccattgcgcg caaggcttct ggtgatttgg cctgggattg ttcgttcatc
     1681 ccgactcctt ttttgtatag ataaaccatc agctgatagt ttacctgaag aatatagaga
     1741 agtacttact taacattttc ccatttggta ctatctaacc ccttttcact attaagaagt
     1801 aatgcctact atgactcaag tcgcgaagaa aattctggtg acgtgcgcac tgccgtacgc
     1861 taacggctca atccacctcg gccatatgct ggagcacatc caggctgatg tctgggtccg
     1921 ttaccagcga atgcgcggcc acgaggtcaa cttcatctgc gccgacgatg cccacggtac
     1981 accaatcatg ctgaaagctc agcagcttgg tatcaccccg gagcagatga ttggcgaaat
     2041 gagtcaggag catcagactg atttcgcagg ctttaacatc agctatgaca actatcactc
     2101 gacgcacagc gaagagaacc gccagttgtc agaacttatc tactctcgcc tgaaagaaaa
     2161 cggttttatt aaaaaccgca ccatctctca gctgtacgat ccggaaaaag gcatgttcct
     2221 gccggaccgt tttgtgaaag gcacctgccc gaaatgtaaa tccccggatc aatacggcga
     2281 taactgcgaa gtctgcggtg cgacctacag cccgactgaa ctgatcgagc cgaaatcggt
     2341 ggtttctggc gccaccccgg taatgcgtga ttctgaacac ttcttctttg atctgccctc
     2401 tttcagcgaa atgttgcagg catggacccg cagcggtgcg ttgcaggagc aggtggcaaa
     2461 caaaatgcag gagtggtttg aatctggcct gcaacagtgg gatatctccc gcgacgcacc
     2521 gtacttcggt tttgaaattc cgaacgcgcc gggcaaatat ttctacgtct ggctggacgc
     2581 accgattggc tacatgggtt ctttcaagaa tctgtgcgat aagcgcggcg acagcgtaag
     2641 cttcgatgaa tactggaaga aagactccac cgccgagctg taccacttca tcggtaaaga
     2701 tattgtttac ttccacagcc tgttctggcc tgcaatgctg gaaggcagca acttccgcaa
     2761 gccgaccaac ttgtttgttc acggctatgt gacagtgaac ggtgcgaaga tgtccaagtc
     2821 tcgcggcacc tttattaaag ccagcacctg gctgaatcat tttgacgccg acagcctgcg
     2881 ttactactac actgcgaaac tctcttcgcg cattgatgat atcgatctca acctggaaga
     2941 tttcgttcag cgtgtgaatg ccgatatcgt gaacaaagtg gttaacctgg cctcccgcaa
     3001 cgcaggcttt atcaataagc gttttgacgg cgtgctggca agcgaactgg ctgacccgca
     3061 gttgtacaaa accttcactg atgccgctga agtgattggt gaagcgtggg aaagccgcga
     3121 atttggtaaa gcagtgcgcg aaattatggc gctggctgat ctggctaacc gctatgtcga
     3181 tgaacaggct ccgtgggtgg tagcgaaaca ggaaggccgc gatgccgacc tgcaggcaat
     3241 ttgctcaatg ggcataaacc tgttccgcgt gctgatgact tacctgaagc cggtactgcc
     3301 gaaactgacc gagcgcgcag aagcattcct taatacggaa ctgacctggg atggtatcca
     3361 gcaaccgctg ctgggccata aagtgaatcc gttcaaggcg ctgtataacc gcatagatat
     3421 gaagcaggtt gaagcactgg tggaagcgtc taaagaagaa gtgaaagcag ctgccgcgcc
     3481 ggtaactggc ccgctggcag atgatccgat tcaggaaacc atcacctttg acgacttcgc
     3541 taaagttgac ctgcgcgtgg cgctgattga aaacgcagag tttgttgaag gttctgacaa
     3601 actgctgcgc ctgacgctgg atctcggcgg tgaaaaacgc aatgtcttct ctggcattcg
     3661 ttctgcttac ccggatccgc aggtactgat tggtcgtcac accattatgg tggctaacct
     3721 ggcaccacgt aaaatgcgct tcggtatctc cgaaggcatg gtgatggctg ccggtcctgg
     3781 cgggaaagat attttcctgc taagcccgga tgctggtgct aaaccgggtc atcaggtgaa
     3841 ataatccccc ttcaaggcgc tgcatcgaca gcgccttttc tttataaatt cctaaagttg
     3901 ttttcttgcg attttgtctc tctctaaccc gcataaatac tggtagcatc tgcattcaac
     3961 tggataaaat tacagggatg cagaatgaga cactttatct atcaggacga aaaatcacat
     4021 aaattctggg cggtggagca acagggaaac gagttgcata tcagttgggg aaaagttggc
     4081 acccaagggc aaagccaggt aaaaaatttt gctgatgttg cagcagcgga aaaagcgaaa
     4141 ctgaagctta tcgcggagaa ggtgaagaag gggtatgtgg agcaagcgaa agataattct
     4201 ttgcaacctt cccaaacggt aacgggctct ctcaaggtag cggacttatc caccattatt
     4261 caggaacaac cctctttcgt agcagaaacc cgtgcggctg acaaaaatac agatgctgtt
     4321 ttaccgtggc tggcgaaaga tattgctgtc gtttttccgc ccgaagttgt acacaccacg
     4381 ttaagccatc gccgctttcc cggagttcct gttcagcaag cagacaaatt gacccaatta
     4441 cgtcgcttag cctgtagcgt gtcgcaacgg gataataaaa cagccacatt tgacttcagc
     4501 acctgttctt tagaatggca aaacaccgtc gcccaggcga tcagtcagat cgacggcctg
     4561 aaaacaacac agctaccatc accagtaatg gctgtactca cggcacttga aatgaaatgc
     4621 acaagatata aagtgcgtga ggatgttatg gatcagatcg tccaggaagg cggtctggaa
     4681 tatgctactg atgtcataat acaccttcaa cagattgata ttaaatggga ttatgcgaat
     4741 aatgtcatta ttattctgcc gtctggcatt gcacctgact acttggagca atattccaga
     4801 tttgaattac gcctacgtaa acatttatca ctggcggaag agtctctctg gcaaaaatgt
     4861 gcacaaaaac ttattgccgc aattccacat attccagaat ggcggcaacc attaattgct
     4921 ttgttattac ccgaaaaacc agaaattgca catgaaattg cccagcgtct actggggcaa
     4981 aaaaaattac cctcgcttga gtggttaaaa atagtggcga ctgatgagca cattcttgcc
     5041 tcattagaaa aatatcacga gccatatgcc atttttgatg attactattg tggtgcgata
     5101 tggtcagcca ccgtattaca ggagcaaggt gttgtagccc tgccccgatt tgctccctat
     5161 gccgcaagtg actactgcgc cgatgtgttg cgtcatatca atcatccgtt cgcattgaca
     5221 ctgcttatac gtgtagccgg gcaaactaaa cgctgtcacg atcggatgac gaaagccatt
     5281 gctgcgttcc cacatgcagc aatggcggca ctgacggaac ttcttgggca aaaagaagag
     5341 aacagttggc gcattatgct aatgacaatg cttatctcac aaccagcact ggcagaacag
     5401 gtcattccct ggctctcgac acccgcagtt gccgtactga aatcatgcca gcaacaactg
     5461 acacagccct caaaccatgc cagcgccgat ctactgccag ccgtagtagt ctcccctccc
     5521 tggctttcga aaaagaaaaa atcgccgatt ccggtgctgg atttagcgcc attaggcatt
     5581 gagccaatct gttatctgac agaagaaatc agtaatcaac ttttggcgaa atatatctgg
     5641 tattcaaaac acatcacggt tagccatgaa gaaagtacta ccaacctgtt ggcaaggatg
     5701 ggttttcaac gacggatcgc tggtacatat attaaagctc ccgaagcggt agttgaggca
     5761 tggctaaatg aagattattc aaccttacta agtgaattta aggtgtttca ttcacctacc
     5821 gggcattatt ggcagttggg gattttgaca acattgccgc tggagaaagc agtaaaagca
     5881 tggaatgccc ttaccctatc tccacatacc gataccgaat actccatgtt acattttgga
     5941 ctcaaagggt tacctgggtt agtaaactca cttgcacgct atccacaaga agccttgccc
     6001 atcacgaatt acttcgcagc gagtgagctg gcacctgccg tcgcccgcgc cttcaacaaa
     6061 ctcaaaaccc tacgacaaga cgcccgtagc tggctgctga aatacccgga acacgccata
     6121 accggcctgc tgccttcggc gctcggcaaa gccggtgaag cacaggataa cgcccgcgct
     6181 gccttgcgta tgcttaccga aaatggtcat cagccattac tgcaagaaat cgcccggcgt
     6241 tataaccagc cggaagtaac cgacgcggtg aacgctctgc ttgcgctcga tcccttagat
     6301 aatcacccga caaaaatccc cactcttccg gccttttatc agccatcgct ctggacgcgc
     6361 ccggtattaa aagcaaacgc ccaatcactg ccagataacg ccctcctcca cctcggtgaa
     6421 atgctccgct tccctcagga agaggctcta tatccgggat tattgcaggt gaaagatgcc
     6481 tgtaccgccg actcactggc tgaatttgcc tgggatctgt ttaccgcctg gcagaccgct
     6541 ggcgcgccgt cgaaagaaag ctgggcgttc actgcgttag gcgttctcgg taacgacgac
     6601 accgctcgca aactgacgcc attaatacgc gcctggcctg gcgaatctca gcataaacgc
     6661 gccaccgttg ggttggatat tctcgccgct atcggtagtg atatcgctct gatgcagctt
     6721 aacggcattg ctcagaaact gaaattcaaa gctttacagg agcgggcgaa agagaaaatt
     6781 gccgacattg ctgaaagtcg cgaactgaca gtggcggagc ttgaagatcg gttagcaccg
     6841 gatctcagtc tggatgataa cggttcgttg ctgctggatt tcggcccacg ccagttcacc
     6901 gtcagctttg atgaaacctt aaaaccgttt gtgcgtgacg cttccggcag ccgtctgaaa
     6961 gacctgccca aaccaaacaa aagcgatgat gaatcacagg cgaacgatgc tgttaaccgc
     7021 tacaaactgc tgaaaaaaga tgcgcgtacc gtcgccgccc agcaggtagc aaggttggaa
     7081 tccgccatgt gcctgcgtcg ccgctggtcg ccagaaaact ttcagctctt cctggttgaa
     7141 catccactgg tacgccactt aacccgccgt ctgatttggg gcgtttatag caccgaaaac
     7201 cagctactga cgtgcttccg cgtagcggaa gataacagct acagcaccgc tgacgatgat
     7261 cttttcaccc tgccggaagg cgatatctct attggtattc ctcacgttct ggaaatatca
     7321 ccgacggatg ctgccgcctt tggtcagctt tttgccgact acgaactgtt accaccgttc
     7381 cgccagcttg accgtaacag ttacgccctg acagaagccg agcgcaacgc cagcgaactg
     7441 acccgctggg caggcagaaa atgcccgagc ggtcgggtaa tggggctggc gaataaaggc
     7501 tggataaagg gcgaaccaca ggatggaggc tggatcggct ggatgatcaa acctctgggc
     7561 cgctggtcgt taatcatgga aatcgatgaa ggctttgcgg taggcatgtc gccagccgaa
     7621 ctgagtgccg agcaactctt aagcaagctg tggctatggg aaggcaaagc ggaaagctat
     7681 ggctggggga gtaattcaac acaggaagcg cagttctccg tactcgatgc catcaccgcc
     7741 agcgagctaa ttaacgatat tgaagccctg tttgaataag gaaagagcat ggacaaggaa
     7801 ttaccgtggc tggctgataa cgcccaactg gaactgaaat ataaaaaggg taaaacacca
     7861 ctcagccatc gcaattggcc gggcgaacca gtgccagtta tcactgaaag catcattcag
     7921 acactgggtg atgaattgct acaaaaagct gagaagaaaa aaaacattgt ctggcgttat
     7981 gaaaattttt cactggagtg gcagtccgcc atcacgcagg ccatcaactt gatcggcgaa
     8041 cacaaaccct caatcccggc acggacaatg gcggcgctag cctgtatcgc gcaaaatgac
     8101 agccaacagt tgctcgacga aatcgtccaa caagaggggc tggaatatgc gacggatgta
     8161 gtgatggcgc gtcagtgcat tgcgcggcgt tatgagagtg attcactggt cgtcactttg
     8221 caatatcagg acgaggacta tggctacggt tatggctccg cgacctataa cgatttcgat
     8281 ctccgtttgc gtaagcatct gtcgctggca gaggaaagtt gctggcagcg ttgtgccgat
     8341 aaactcattg ccgcactgcc aggaataccc aaaattcgcc gtccttttat tgcgctgatc
     8401 ctcccggaaa agcctgaaat agccaatgaa ctcgccagtc ttgaaagctc ccggtcaagt
     8461 ctccattcaa aggagtggtt aaaggtcgtt gctactgata atacggcggt aaaaaaactc
     8521 gaacgctact ggagccagga tatatttagc gatcgagaag ccagctacat gtcgcatgaa
     8581 aaccacttcg gctacgcggc ctgcgctgcc cttttgcgcg aacaaggact ggcagtcgtt
     8641 ccgcgcctcg cgatatatgc ccataaagaa gattgcggca gtctgctggt acaaattaac
     8701 catccgcaag tcatccgcac cttgctgtta gttgctgata aaaacaaacc cagcctgcaa
     8761 cgtgtggcta aatacagtaa aaacttcccc catgcgacac tcgccgcact ggcagaactg
     8821 ctggcgttaa aagaaccacc agcccgccca ggttatccaa tcatcgaaga caaaaagctg
     8881 cctgcacagc aaaaagctcg agatgaatac tggcgtacgc tgttacaaac gctgatggca
     8941 tcgcagccac aactggcagc agaagtgatg ccgtggttaa gtactcaagc ccgggcagtg
     9001 ctgaagagtt atttatcggc accgcccaaa ctggttattg atggcaccga taacagcaat
     9061 ctgcctgaaa tcctcgtttc accaccgtgg cgtagtaaga aaaaaatgac agctccacgt
     9121 cttgatttgg caccgctcga attaacgccg caagtttact ggcaacctgg cgaacgagag
     9181 aggcttgccg ccactgagcc tgcccgttat ttcagcacgg aatctcttgc ggaacgcatg
     9241 gaacaaaaaa gtgggcgagt tgtattacag gaactgggtt ttggggatga tgtatggctg
     9301 tttttgaatt atatactccc cggaaaactg gatgctgcac gcaattcact cattgttcag
     9361 tggcattact accaggggcg ggttgaagag atcctgaatg gctggaactc cccggaagca
     9421 caattagcag aacaggcgct ccgcagcggt cacatagaag cgttaattaa catatgggaa
     9481 aatgacaact actcacgtta tcgtgcggaa aagagtgtct ggaacctgta tttattggca
     9541 cagttgccgc gtgagatggc tttgaccttc tggctgcgta tcaatgagaa aaaacatctg
     9601 ttcgcgggtg aggactattt tctcagtatc ctcgggttgg atgcgctacc aggtctgatg
     9661 ttggcttttt cacatcgtcc aaaagaaaca tttccgttaa ttttaaattt tggcgcaaca
     9721 gaactggccc tgcccgttgc ccgcgtctgg cgacgtttcg cggtgcagcg tgatctggct
     9781 cgccagtgga ttttacaatg gccggaacat acggctactg cacttatccc tcttgtcttt
     9841 accaaaccca gcgataatag cgaagccgca ttacttgccc tgcgtttact gtacgaacag
     9901 ggacatggcg agttgctgca aaccgtggca aaccgctggc aacgcgctga tgtatggcct
     9961 gccctggagc agttgcttaa acagggtcca atggacattt acccggcacg cattccaaaa
    10021 gcccctgatt tctggcatcc ggcaatgtgg tccaggccgc gacttatcac taataatcag
    10081 cctgttaccg atgacgctct ggaaattatc ggcgaaatgc tgcgctttac ccagggggga
    10141 cgtttttata gcgggctgga acaactgaaa acgttctgcc agccacaaac gctggcagct
    10201 tttgcctggg atctcttcac tgcgtggcaa caagctggtg cccccgcaaa agacaactgg
    10261 gcatttctgg cgttaagtct ctttggtgac gaaagcacgg cacgggatct gacgacacag
    10321 atcctcgcct ggccacaaga aggcaaatct gcccgtgctg tcagcggcct gaacatcctt
    10381 accctgatga ataatgatat ggcgctgata cagctgcatc atatatcgca acgggctaaa
    10441 tcccgcccct tacgtgataa cgcggcggaa tttcttcagg tggtcgcaga aaatcgcggg
    10501 ctaagccagg aagagctagc ggacagatta gtcccaaccc tgggccttga tgatccgcag
    10561 gcgttgagtt ttgattttgg tccccggcat tttaccgttc gcttcgatga aaaccttaac
    10621 ccggttatct ttgatcagca aaacgttcgc cagaaaagcg ttccccggtt gcgcgccgat
    10681 gacgatcaac tgaaagcgcc cgaggcactg gcccgactaa aagggctaaa aaaagatgct
    10741 actcaggtga gcaaaaacct gctcccgcgt cttgaagctg ccctacgtac cacccgacgc
    10801 tggtcgctgg cagattttca ttctctgttt gttaatcatc cctttacccg cctggttacc
    10861 cagggattaa tatggggggt ttatccggca aatgaaccgc gtcgtttact caacgccttt
    10921 cgtgtggccg cagaggggga gttctgcaat gcgcaggatg agcccattga ccttcctacg
    10981 gatgctctga ttggcattgc ccacccgtta gaaatgacag cagaaatgcg cagtgaattt
    11041 gcacagcttt ttgccgatta cgaaattatg ccgccttttc gccagttgtc gcgccgcacg
    11101 gtgctgctca cacctgacga gtcaaccagt aacagcctga ctcgctggga aggtaaatcc
    11161 gctaccgttg ggcaacttat gggaatgcga tacaaaggct gggagtcagg ctatgaggac
    11221 gcatttgtct atgacctggg tgagtaccgg ctggtcctta agttttcacc cggttttaac
    11281 cactacaatg ttgatagcaa agcgctaatg agcttccgtt ctcttcgagt gtaccgtgac
    11341 aataaatccg tcacttttgc cgaacttgat gtgtttgatt tgagtgaggc gttaagcgca
    11401 cctgacgtca ttttccatta aaacacagcg ggcagtgatg caactgcccg ttatcaacac
    11461 gacttaccag cggggaaagc gatgatcgtg caaaaagagc tggttgctat ttacgattat
    11521 gaggtccctg tacctgaaga tccgttttcc tgcagacttg agatccataa atgctctgaa
    11581 ttatttacag gttccgtcta tcgactggag cgattccggc tacatccaac atttcatcaa
    11641 cgtgatcgag aagatgctga cccgctaata aatgatgcgt tgatttatat aagagatgag
    11701 tgtattgatg agcggaaatt acgaggtgaa tcacctgaaa ctgtaatagc aatttttaat
    11761 cgtgaactac agaatatatt catccaacaa atagaaaaat agctttatta aagggggaaa
    11821 cggtattccc ccttagatgc gtttcaattt gcccgttact tcaacaaatc aatcaaccgt
    11881 aacagagcca ctaataactc cagtacggca atgagcacga ttaacttttg tgtcagcgtg
    11941 tccatccgct ctccttatta aggagcgcaa caccagcacc ttacccttac actgcctgtc
    12001 gatactggtt tacgtatgtt ggtacgaaac gttgctctcc ggatcagggc accgcctgcg
    12061 gcaccacccg tccagcagcc aggacttaaa attggtccga tccggcacaa cctcccaacg
    12121 ggcaggatta taaatctcat tcactgaatt gcaatggagt gtggatatgg ttataattta
    12181 ttcgttggta cgaaatgttg cacaatcagc aagttagcgt cactgtagta aataaaagaa
    12241 cctgcctcac cagcaggttt ttttattttc tgcgatctac tttccagata ttttttgcca
    12301 atagcccacc ataaatattc acatcccggt caaatggtg
//
LOCUS       DAPGOF010000145        12292 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.146, whole genome shotgun sequence.
ACCESSION   DAPGOF010000145 DAPGOF010000000
VERSION     DAPGOF010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.146"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..907
                     /locus_tag="RHV13_003587"
     CDS             <1..907
                     /locus_tag="RHV13_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HDV1243081.1"
                     /translation="GLELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHAT
                     LKEIKDLAKNITQQYGTPDLCIEAMRKKNLKTMLFISQLKAKTNFQVVGLTMKCYSPL
                     CKNASRMDQNLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGAYI
                     ALNLEGSSQERTFSLSIAENLIEKIQSETDIPIVIVHGPKGEGKARALVDCYNNVYRL
                     SLSPSIKRSAAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESV
                     VVGQKIDNISLDEFAKALKSVLARI"
     gene            complement(1169..9151)
                     /locus_tag="RHV13_003588"
     CDS             complement(1169..9151)
                     /locus_tag="RHV13_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HDV1243082.1"
                     /translation="MATKKRSGEEINDRQILCGMGIKLRRLTAGICLITQLAFPMAAA
                     AQGVVNAATQQPVPAQIAIANANTVPYTLGVLESAQSVAERFGISVAELRKLNQFRTF
                     ARGFDNVRQGDELDVPAQVSENNLTPPPGNSSGNLEQQIASTSQQIGSLLAEDMNSEQ
                     AANMARGWASSQASGAMTDWLSRFGTARITLGVDEDFSLKNSQFDFLHPWYETPDNLF
                     FSQHTLHRTDERTQINNGLGWRHFTPTWMSGINFFFDHDLSRYHSRAGIGAEYWRDYL
                     KLSSNGYLRLTNWRSAPELDNDYEARPANGWDVRAEGWLPAWPHLGGKLIYEQYYGNE
                     VALFDKDDRQSNPHAITAGLNYTPFPLMTFSAEQRQGKQGENDTRFAVDFTWQPGSAM
                     QKQLDPNEVAARRSLAGSRYDLVDRNNNIVLEYRKKELVRLTLTDPVTGKSGEVKSLV
                     SSLQTKYALKGYNVEATALEAAGGKVVTTGKDILVTLPAYRFTSTPETDNTWPIEVTA
                     EDVKGNFSNREQSMVVVQAPTLSQKDSSVSLSTQTLSADSHSTATLTFIAHDAAGNPV
                     IGLVLSTRHEGVQDITLSDWKDNGDGSYTQILTTGAMSGTLTLMPQLNGVDAAKAPAV
                     VNIISVSSSRTHSSIKIDKDRYLSGNPIEVTVELRDENDKPVKEQKQQLNTAVSIDNV
                     KPGVTTDWKETADGVYKATYTAYTKGSGLTAKLLMQNWNEDLHTAGFIIDANPQSAKI
                     ATLSASNNGVLANENAANTVSVNVADEGSNPINDHTVTFAVLSGSATSFNNQNTAKTD
                     VNGLATFDLKSSKQEDNTVEVTLENGVKQTLIVSFVGDSSTAQVDLQKSKNEVVADGN
                     DSATMTATVRDAKGNLLNDVKVTFNVNSAAAKLSQTEVNSHDGIATARLTSLKNGDYT
                     VTASVSSGSQANQQVNFIGDQSTAALTLSVPSGDITVTNTAPQYMTATLQDKNGNPLK
                     DKEITFSVPNDVASRFSISNGGKGMTDSNGVAIASLTGTLAGTHMITARLANSNVSDT
                     QPMTFVADKDRAVVVLQTSKAEIIGNGVDETTLTATVKDPFDNVVKNLSVVFRTSPAD
                     TQLSLNARNTNENGIAEVTLKGTVLGVHTAEAILLNGNRDTKIVNIAPDASNAQVTLN
                     IPAQQVVTNNSDSVQLTATVKDPSNHPVAGITVNFTMPQDVAANFTLENNGIAITQAN
                     GEAHVTLKGKKAGTHTVTATLGNNNASDAQPVTFVADKDSAVVVLQTSKAEIIGNGVD
                     ETTLTATVKDPFDNAVKDLQVTFSTNPADTQLSQSTSNTNDSGVAEVTLKGTVLGVHT
                     AEATLPNGNNDTKIVNIAPDASNAQVTLNIPAQQVVTNNSDSVQLTATVKDPSNHPVA
                     GITVNFTMPQDVAANFTLENNGIAITQANGEAHVTLKGKKAGTHTVTATLSNNNTSDS
                     QPVTFVADKTSALVVLQISKNEITGNGVDSATLTATVKDQFDNEVNNLPVTFSTASSG
                     LTLTPGESNTNESGIAQATLAGVAFGEQTVTASLANTGASDNKTVHFIGDTTAAKIIE
                     LTPNPGSIIAGTPQNSTGSVITATVVDNNGFPVKGVTVNFTSNAATAEMTNGGQAVTN
                     EQGKATVTYTNTRSSIESGARPDTVEASLENGSSTLSTSIQVDADASTAHLTSLYTLY
                     DTQLAGEDTTLYITVNDIYGNGVPLHQVTLSVSPSEGVTLSNNGINTTNHDGYLYASM
                     TATKAGVYQVTATLENGDSIQQTVTYVPNVANAEITLAASKDPVIADNNDLTTLTATV
                     ADTEGNAIANTEVTFTLPEDVKANFTLSDGGKAVTDTEGKAKVTLKGTKAGAHTVTAS
                     MAGGKSEQLVVNFIADTLTAQVNLNVTEDNFIANNVGMTRLQATVTDGNGNPLANEVV
                     TFTLPADVSASFTLGQGGSAITDINGKAEVTLSGTKSGTYPVTVSVNNYGVSDTKQVT
                     LIADAGTAKLASLTSVYSFVVSTTEGATMTASVTDANGNPVKGIKVNFRGTSVTLSST
                     SVETDDQGFAEILVTSTEVGLKTVSASLADKPTEVISRLLNAKADINSATITSLEIPE
                     GQVMVAQDVAVKAHVNDQFGNPILNESVTFSAEPPEHMTISQNIVSTDTHGIAEVTMT
                     PERNGSYMVKASLANGSSYEKDLVVIDQKLTLSASSPLIGVNSPTGATLTATLTSANG
                     TPVEGQVINFSVTPEGATLSGGKVRTNSSGQAPVVLTSNKVGTYTVTASFHNGVTIQT
                     QTTVKVTGNSSTAHVASFIADPSTIAATNTDLSTLKATVEDGSGNLIEGLTVYFALKS
                     GSATLTSLTAVTDQNGIATTSVKGAMTGSVTVSAVTTAGGMQTVDITLVAGPADASQS
                     VLKNNRSSLKGDFTDSAELHLVLHDISGNPIKVSEGLEFVQSGTNVPYVQVSAIDYSK
                     NFSGEYKATVTGGGEGIATLIPVLNGVHQADLSTTIQFTRAEDKIMSGTVSVNGTDLP
                     TTTFPSQGFTGAYYQLNNDNFAPGKTAADYEFSSSASWVDVDATGKVTFKNVGSNWER
                     ITATPKSGGPSYVYEIRVKSWWVNAGDAFMIYSLAENFCSSNGYTLPRADHLNHSRSR
                     GIGSLYSEWGDMGHYTTEAGFQSNMYWSSSPANSNEQYVVSLATGDQSVFEKLGFAYA
                     TCYKNL"
     gene            complement(9465..9540)
                     /locus_tag="RHV13_003589"
     tRNA            complement(9465..9540)
                     /locus_tag="RHV13_003589"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9505..9507),aa:Asn,seq:gtt)
     gene            complement(9641..10438)
                     /gene="mtfA"
                     /locus_tag="RHV13_003590"
     CDS             complement(9641..10438)
                     /gene="mtfA"
                     /locus_tag="RHV13_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HDV1243083.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            10532..10621
                     /locus_tag="RHV13_003591"
     tRNA            10532..10621
                     /locus_tag="RHV13_003591"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10566..10568,aa:Ser,seq:cga)
     gene            complement(11191..11721)
                     /locus_tag="RHV13_003592"
     CDS             complement(11191..11721)
                     /locus_tag="RHV13_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HDV1243084.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIAAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
     gene            complement(12064..>12292)
                     /locus_tag="RHV13_003593"
     CDS             complement(12064..>12292)
                     /locus_tag="RHV13_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HDV1243085.1"
                     /translation="KKGVRYLFECKDPESKAPKYIQFSDHIIAPRKSSHFHIFMGNDS
                     QQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT         3030 a         2866 c         2921 g         3475 t
ORIGIN      
        1 ggggcttgaa ttgcataaaa ttaagcatct tttaattgta acgtcgacaa ttaatttaga
       61 agtctttaat gcgctcaaac ttgaacagac taaattaaca ttagtcacaa tgactatgca
      121 ggatcacgca acattaaaag aaataaaaga tttagcgaag aacataacac agcaatacgg
      181 tacgccggat ctttgtatag aggcgatgcg taaaaagaac ctgaaaacga tgttatttat
      241 cagtcagttg aaagcaaaaa cgaattttca ggttgttggt ttaaccatga agtgctattc
      301 ccctttgtgt aagaacgcgt cccggatgga tcagaatctc cgggctcctg tacccatgac
      361 atgggcattt atgatgcggg aggcgggttt tccagcagtc aggccaatat atgaattgcc
      421 actaagtgag gatgtactcg atgaggtgcg cgaggaaatg cgatcgttag gagcttacat
      481 tgcgctcaat ttagaaggta gctcgcagga acgtacattt tcactatcga ttgcagaaaa
      541 tctaatagaa aaaattcaaa gtgaaacaga tataccaata gtgatcgttc atggaccaaa
      601 aggggaaggt aaagccaggg cattagtgga ttgttataat aatgtctacc gtttatcctt
      661 atcaccctcg attaaacgtt cagcagcaat cataaaagat gcttatatcg caataactcc
      721 tgacacctca atattacata tggcaagtgc ctataatacc cccgttgttg caatttatgc
      781 tgattacaaa acgcgatggc ccgcaatggc cgatgtttcg gagtcagtcg tcgttgggca
      841 aaaaattgac aatataagtc tggatgaatt cgcaaaggca ttaaaaagtg ttttggcgag
      901 aatatgaggc agtacttatg aatagtaatg ccgatctgtt atgaacggtg gtgtcatcaa
      961 tactctgagg ctggtcaatc atcacagaaa accagccttt agatcaaagc agtactcacc
     1021 gaaaatgatc atagtcacat cgaacgcgat tctggcggtg cagcattttt atttattcat
     1081 tttctgtgag agaagggcaa gctctataga gggaatgtaa aaaagagtgc cccgaatgga
     1141 gcactcttta agaaattata acggaaaatc agaggttttt ataacatgtc gcataagcaa
     1201 acccaagctt ttcaaatacg ctttgatcac ctgttgccag ggaaactacg tattgttcgt
     1261 ttgagtttgc gggactagat gaccaataca tatttgattg aaaaccagcc tcagtcgtgt
     1321 aatgccccat atctccccat tcactgtaca gtgacccgat gcctcgggaa cgactatggt
     1381 ttaaatgatc tgcacgggga agtgtgtagc cattgctact gcaaaaattt tcagcaaggc
     1441 tgtatatcat gaaagcatcg ccggcgttca cccaccaact cttcacacgg atttcgtata
     1501 catagctagg gcctcctgat tttggcgtcg ccgtaatcct ttcccaattg ctgccgacat
     1561 ttttaaatgt cactttaccg gtagcatcaa catcgaccca ggaggcagag cttgaaaact
     1621 cataatcagc cgccgttttt ccaggggcaa agttgtcatt attcaactga taatacgccc
     1681 cggtgaatcc ctgcgaaggg aatgtagttg tcggtaggtc agtaccattg actgacactg
     1741 taccgctcat tattttgtct tctgcgcgag tgaattgtat tgtggtactc agatcagctt
     1801 gatgaacacc attcaataca gggatcagcg ttgcgatacc ctctccgcct cctgtaacag
     1861 tggctttgta ctcgcctgag aaatttttac tgtagtcaat tgcactaact tgcacatagg
     1921 gcacgttggt acctgactgc acaaattcca gcccttcaga aactttgatc ggattgcctg
     1981 atatatcgtg cagaacaaga tgtagctcag cactatcggt aaagtctcct ttcaatgatg
     2041 accgattgtt cttaaggacg gactgcgagg cgtctgccgg gcctgccacc agcgttatat
     2101 ctactgtttg cattccacca gcggtcgtga ctgcgcttac cgtgacgcta cctgtcatcg
     2161 ctcctttcac gcttgttgtc gcgattccgt tttgatcggt caccgctgtt aatgacgtta
     2221 atgtggcaga gccgcttttt aaggcgaagt acacagtgag accttcgatc aggttaccac
     2281 tgccatcctc aaccgttgcc tttaaggtac ttaaatcagt gttggtggcg gcgatagtcg
     2341 atggatcagc gataaagcta gcaacatggg cggtgcttga gttgccagtg actttcacgg
     2401 ttgtctgtgt ctgtattgtt acaccgttat ggaacgatgc agtcaccgta tatgtaccga
     2461 ctttattgct ggtcagaacg actggtgctt gacctgaaga gttagttctc acttttccgc
     2521 cacttaacgt tgccccttct ggcgttacgc taaagttgat gacctgaccc tccactggag
     2581 tgccatttgc agaagttagc gttgccgtca gagttgcacc tgttggggaa ttgacaccga
     2641 taagcgggct ggacgccgag agtgtcagtt tttgatcgat taccaccaga tccttctcat
     2701 aagaggatcc attcgccagg gatgctttca ccatatacga accgtttctt tcgggcgtca
     2761 tagtgacctc ggctatacca tgcgtatcag tagagacaat attttggctg atggtcatgt
     2821 gctctggtgg ctctgcactg aatgttacag attcattaag aatcggattg ccaaactggt
     2881 cgttgacgtg agctttaact gctacgtctt gtgcgaccat gacctgacct tcaggtatct
     2941 ccagactggt aatcgttgca gaattaatat ctgcttttgc attcagtaat cgcgagatga
     3001 cttcagtagg tttatctgcc agagaggctg aaactgtttt cagtccgacc tcggtgcttg
     3061 tcacaagaat ttcagcgaaa ccctgatcat ccgtttcaac gctggtgctg cttagcgtga
     3121 cggaggttcc gcggaaatta acttttatgc cttttaccgg gttgccgtta gcgtcagtga
     3181 cgcttgccgt catggttgcg ccctcggtcg tgctgacgac gaatgaatat acagaggtta
     3241 aggaagccag ttttgcggta cctgcatcgg caatcaaagt cacctgtttc gtatcactga
     3301 caccataatt gttcacgcta actgtcacgg gataggtgcc ggattttgta ccgctcagtg
     3361 taacttcagc cttgccgttg atatcagtaa tggcggaacc gccttgtccg agagtgaagc
     3421 ttgcactcac gtctgccggt agcgtgaatg tcaccacctc attggctaac gggttgccgt
     3481 ttccatcagt caccgttgcc tgcagcctgg tcatcccgac gttattagcg ataaagttgt
     3541 cctcggtaac gttaagatta acctgcgctg tgagcgtatc cgcaataaaa ttcaccacca
     3601 actgctcact cttaccgcca gccatcgatg ctgtaacagt atgagcgcct gcttttgtac
     3661 ctttcagcgt gactttcgct ttgccttcag tatcagtaac cgctttaccg ccatcgctca
     3721 gcgtgaagtt cgccttcaca tcttccggca gagtaaatgt tacttcagtg ttggctatcg
     3781 cattgccctc tgtatcagcg actgttgctg ttagtgtcgt gagatcgtta ttgtcggcaa
     3841 tcaccggatc cttcgaggct gccagcgtga tttcggcatt cgcgacgttc ggcacatagg
     3901 tcaccgtttg ttgtatcgaa tcgccatttt cgagggttgc cgtcacttga taaactccgg
     3961 cttttgtagc agtcatgctt gcgtataaat agccgtcgtg gttggtagta tttataccgt
     4021 tattactgag ggtcacgcct tcactcggcg aaacgctgag ggttacctga tgcagaggta
     4081 caccattgcc gtaaatatca ttcaccgtaa tgtacagagt ggtatcctcg cctgccaatt
     4141 gtgtgtcata aagggtataa agcgaggtga gatgtgccgt agacgcatca gcgtcgactt
     4201 gaatggacgt gctaagcgtg gagctaccat tttccagact ggcctcaacg gtatccggtc
     4261 tcgctcctga ttctatcgag gagcgggtat tggtataagt gacggtagcc ttaccctgtt
     4321 cgttcgttac ggcttggccg ccattcgtca tttcggctgt cgctgcgttg ctggtgaagt
     4381 tcacagtcac acctttcacc ggaaagccat tattatcaac gactgtagcg gtgattacgc
     4441 taccggtgct gttctgtggg gtaccggcga ttatgctgcc tgggttaggc gtcaactcga
     4501 taatttttgc cgccgttgtg tcgccaataa agtgcacagt tttgttgtcg ctggcaccag
     4561 tattagccag tgatgcagta acagtctgct caccaaaggc aacgcctgcg agagtggcct
     4621 gcgcgatgcc agactcattg gtattacttt cccctggggt cagggtgagg cctgaagaag
     4681 ctgtgctgaa tgttaccgga agattgttca cctcattgtc gaactgatct ttgaccgttg
     4741 cagttagcgt tgcgctatcg acgccattac ctgtgatctc attttttgat atctgaagaa
     4801 caaccagagc cgaggttttg tccgccacaa acgttaccgg ctgtgaatca ctggtattgt
     4861 tattactcag cgttgcggta accgtatgcg tacccgcttt tttacctttg agcgtgacat
     4921 gcgcttcccc attggcctgg gtgatggcaa taccgttatt ttcgagggta aagtttgccg
     4981 ccacgtcctg tggcatggtg aaattcaccg ttattcccgc caccggatga ttcgacggat
     5041 ctttcaccgt cgccgtcagc tgcacgctgt cgctgttatt cgtcaccacc tgttgagcag
     5101 ggatgttcag cgtaacctgc gcgttgctgg catcgggcgc aatattgact atcttcgtat
     5161 cgttgttgcc gttaggcagt gtggcttcgg ctgtatgaac acccaaaacc gtgcccttaa
     5221 gggtaacttc agcaacacca ctgtcgttag tattgcttgt gctctgacta agttgagtat
     5281 ctgccggatt ggtactgaag gtgacttgta gatcttttac tgcattatca aaaggatctt
     5341 tcactgttgc tgtcagagtc gtctcatcca cgccattccc aatgatttcc gctttcgatg
     5401 tttgcagaac gacaactgcg ctgtccttat ctgccacgaa ggtgaccggt tgcgcatcgc
     5461 tggcattatt gttacccagc gttgcggtaa ccgtatgcgt gcccgctttt ttgcctttga
     5521 gggtgacatg cgcttcgcca ttggcctgag tgatggcaat accgttattt tcaagggtaa
     5581 agtttgccgc cacgtcctgt ggcatagtga agttcaccgt tattcccgcc accggatgat
     5641 tcgacggatc tttcaccgtc gccgtcagct gcacgctgtc actgttattc gtcaccacct
     5701 gttgtgcagg gatgttcagg gtgacctgcg cgttgctggc atcgggcgca atattgacta
     5761 tcttcgtatc tctgttgccg ttaagcagta tggcttcggc tgtatgaaca cccaaaaccg
     5821 tgcctttaag ggtaacttca gcaataccgt tctcattagt attacgcgcg ttcagactca
     5881 gttgcgtgtc tgcgggggag gtgcggaaga ctactgacag atttttaacc acgttatcaa
     5941 aaggatcttt cactgttgct gtcagagttg tctcatccac gccattccca atgatttccg
     6001 ctttcgatgt ttgcagaacg acaaccgctc tgtctttatc cgccacaaac gtcattggct
     6061 gtgtatcgct gacattgctg ttagccagac gagccgtgat catatgcgtg cccgctaacg
     6121 tgccggtcag ggaggcgatt gcaaccccgt tactatccgt catgcctttt ccgccgttgc
     6181 taatcgagaa ccgacttgcg acgtcgtttg gcacagagaa ggtgatttct ttatctttta
     6241 gtgggttgcc atttttatcc tgcaaggttg cagtcatata ttgcggagct gtgttggtga
     6301 cggtgatatc acctgaaggc acactgaggg tcagggcagc agtactttga tcaccaataa
     6361 aattcacctg ttgattggct tgggaaccag agctcacaga ggccgtaacc gtataatcac
     6421 cgtttttcaa actggtcagc ctagctgtgg cgatcccgtc gtggctattc acttcggttt
     6481 ggctcagttt cgctgctgct gaattgacat tgaaggtgac cttgacgtca ttgagcaggt
     6541 tgccttttgc atcccgaact gtcgcggtca ttgtggcact gtcattgccg tcagcgacca
     6601 cttcattttt cgacttctgc agatcaacct gcgcggtact cgagtcgccg acaaaactga
     6661 cgattaacgt ttgtttcacg ccattttcaa gggtgacttc aaccgtgttg tcttcctgct
     6721 tactactttt cagatcaaaa gtcgccagac cattaacatc cgtttttgcg gtgttttgat
     6781 tgttaaagga agttgccgat ccgcttaata ccgcaaacgt gacggtatga tcattgattg
     6841 ggttgcttcc ttcatcagcg acattgaccg agacggtgtt tgctgcattc tcattggcga
     6901 gcacaccatt attgctggca gataatgtcg caattttcgc tgactgcggg ttggcgtcga
     6961 tgataaatcc agcggtatgc aaatcttcat tccagttttg cattaacagc ttcgcagtaa
     7021 gcccactgcc tttggtatag gcggtatagg ttgccttata gacgccatct gcggtttctt
     7081 tccagtctgt agtgacacca ggtttcacgt tgtcgatgct gactgcggta ttcagttgct
     7141 gtttttgctc cttaacaggt ttgtcatttt catctctcag ttctaccgtc acctcgatag
     7201 gattcccgga gagataacgg tccttatcaa tcttaattga cgagtgagtc cgggatgacg
     7261 aaacagaaat gatattcacc acggcggggg ctttagccgc atccacaccg ttcagctgtg
     7321 gcatcagcgt cagcgtgcca gacatcgctc ctgtggtcag gatctgggta tagcttccgt
     7381 caccattatc tttccagtca gaaagggtga tgtcctgaac accttcgtga cgcgtcgaga
     7441 gcaccagccc gataacagga ttacctgctg catcatgcgc aataaaagtc agtgtggcgg
     7501 ttgaatggga atccgcgctc aacgtctggg tacttaacga taccgaggaa tctttctggc
     7561 ttagcgtagg tgcctgaacg actaccatgc tctgttcacg attcgaaaaa ttgcctttga
     7621 catcttcagc ggtgacttca atcggccagg tgttatcggt ttctggcgta ctggtgaacc
     7681 ggtacgccgg cagggtaacc agaatatctt tacccgttgt aaccacttta ccaccggcag
     7741 cttccagagc ggtggcttcg acgttatagc ctttcagggc atatttggtt tgtagcgacg
     7801 aaaccagtga tttcacttct cctgacttcc ctgtcacggg gtctgtcagg gtcaggcgaa
     7861 ccagttcttt tttgcgatat tccagaacga tgttgttgtt gcgatccacc agatcataac
     7921 ggctgcctgc aaggctacgc cgtgcagcga cttcattcgg gtcaagctgt ttctgcatcg
     7981 cgctgccagg ttgccaggta aaatcgacgg caaaacgggt gtcattttcg ccctgtttac
     8041 cctgacgttg ctccgcgctg aaggtcatca gcgggaaggg ggtatagtta agtccagcgg
     8101 ttatggcatg aggattactt tgccgatcat ctttatcgaa cagggccact tcattgccat
     8161 aatactgttc atagatcagt ttaccgccaa ggtgcggcca ggcgggtaac cagccttctg
     8221 cgcgtacatc ccagccattg gccgggcgtg cttcataatc gttgtccagt tcaggtgcgc
     8281 tgcgccagtt tgtcagtcgc aaatagccgt tactgcttaa ttttagatag tcgcgccagt
     8341 actccgcgcc aatgccggcg cgggagtggt aacggctaag atcgtggtcg aaaaagaagt
     8401 tgatgcccga catccatgtg ggagtgaaat gacgccaacc caagccgttg ttaatctgcg
     8461 tacgctcgtc agtacgatgg agagtatgct gactgaaaaa gagattatca ggcgtttcat
     8521 accacggatg gagaaaatcg aactgggagt tcttcaggct aaaatcttca tccacgccca
     8581 gcgtgattct tgcggtaccg aagcggctta accagtctgt cattgcgcct gaagcctgag
     8641 aagaggccca tccacgcgcc atatttgccg cttgctcgct gttcatatcc tcggcgagca
     8701 gagacccgat ttgctgtgaa gtactggcta tctgttgctc aaggttgcca ctgctattac
     8761 ccggtggcgg ggttaaatta ttttcactaa cttgtgccgg gacatccagt tcatcaccct
     8821 ggcggacatt atcaaaacct cgagcaaacg tacgaaactg gttgagtttg cgtaactcag
     8881 ccaccgaaat accgaaacgt tcggcaacgc tttgggccga ttccagcact ccaagggtgt
     8941 agggcaccgt attggcattt gcaatggcaa tttgtgcagg aactggttgt tgggttgcgg
     9001 cgtttaccac accttgtgct gccgcagcca tagggaacgc aagttgagtt atcaggcaga
     9061 tacccgcagt taagcggcgt agtttaattc ccatcccgca taagatttgt cggtcattta
     9121 tttcttctcc acttctcttc ttcgtagcca tgcttacctt ccctgaacga gctaacataa
     9181 atgcgccctt tctagcacag cgaaaaaatg taaatcctga tagcaatatg attgaaaatt
     9241 aataactttt cgttaggcag ttttgggtgt gagttgcaag aggggagact actgaataac
     9301 tcaagtttta taatcgaggg gaaaatggtg atggcgttca tagcaaaacg ccctcaacca
     9361 taaaggtcga gggcgcttaa gatgttaaaa acccgctatc cgttaaaaaa caatgttcaa
     9421 ctaaggtcag tgacattgcg ctaaaaaagc gaatttttag aatttggctc ctctgactgg
     9481 actcgaacca gtgacatacg gattaacagt ccgccgttct accgactgaa ctacagagga
     9541 atcgtgtgaa cggggcgcat attacttagc ggtaccttgt ctgtcaacac taaatttcat
     9601 aagtcatttc aattggttaa ttaatctgca aagttgttaa ttaatgaaca ttcgtcgccg
     9661 aaaacgagtc tgtatcatta gcgtgatgca gtctctgcaa aggatcttgt tgataaaatt
     9721 ggcagaaacg ttgccacaat gaagggaaac gaggagcaaa aagttctggg gcgctaaaga
     9781 aatattcaga aagtacggca aaacattcag caggatcact ggcagcataa gcatcaatgc
     9841 tcgccgcatt ctcgccaacc aattcgattt cttcctgaat gttgttcatt gcagcatgaa
     9901 gatcgtgttc ccagccagca acctcacgca acggaataaa gggaacaccg ctggcgcgat
     9961 cgccgttacg ggtgtccagc ttatgagcga cttcatgaat aatcaggtta aaaccagaag
    10021 catcaaaaga atcttgtata tccaaccagt tcaaaacgat aggcccttgc tgccagctct
    10081 gacctgactg aacaatacgt tggttatgca ccagaccgat atcgtcttcc cattcatcat
    10141 cgaccacaaa tggcgcagga taaattaaga cttcatgaaa accatccagc cattccagtc
    10201 ctaactccag aacgggtagg caaaatagaa gtgctatccg gcagcttctt aatgaatcca
    10261 gttcaaagcc ctgtaaagga acaagccgct tttgctgtaa aaaacgttcg gcaagagtga
    10321 ctaatttgct ttgttcctgt tccgtcagac acgttaaaag ggggatcgat agtgcttcct
    10381 gccagggaag ggcagtttga tgtgctgatt cttgtacttt ccagggccac ttaatcatcg
    10441 ttttgctcgc aaactcgtca cttgaacaaa attgcacgga cagggactgt taaaatgcca
    10501 aatttcctgg catcatggca accatctgaa cggagagatg ccggagcggc tgaacggacc
    10561 ggtctcgaaa accggagtag gggcaactct accgggggtt caaatccccc tctctccgcc
    10621 actttatcaa tgacttatct cccgacttcc cgccttgctt ttcctaaaca gaacaatcgt
    10681 agaatattct tgaagggtta gatcgtcact gttttctgtt cgatactgtg acattcagca
    10741 cttgattcgc tatggatctg acaggaagct ttcgagcgaa aatgtgcagt tattcagtcg
    10801 ttttcttatc gctcaccatt cttcttttag acattcatcc tatgaaactg ccgcaaagtt
    10861 ggtggtggga actgaagttg cgtagagaag gggtcaataa ttgagtcaaa aaaggaacgc
    10921 ggccgtcaga ttaaagccgc gggacaaagt ccataatttc caagagttgt tgaatttccc
    10981 agcttttttg aaagcctttg tatcatacta acaagtaaaa aaggtttaaa taccaaaatt
    11041 aacctgtatt tttgataagt cttaccattg tacgatttac acagtttctg ctcctccttg
    11101 ttgatagtgg aaagggtgca ttgtgcactt gcgtaaacat ttatgacgcg ttgatgtcca
    11161 tggttcacat ttccttgtca ggggatcgac ttatttgcgg cgaggcatca ttcgtaacag
    11221 agtattatct ttctgaatgt agtgatgaaa gagtgctgcg ccagcgtgag ctgcgattaa
    11281 aaagtagcct atatttgccc aggtttcgtg aatatttttt atcagtgctt taatttcgct
    11341 gtttggggta acaaagggag acacattgaa accaaggaaa ctccacgatt ttccactgta
    11401 agccattaaa gcaatcccca gcagaggaag cgcaaggaag gttatataaa gcatgatgtg
    11461 cattagcgaa gccgctttca tttgccaggc aggtggcggt ggcacaatag aaggatcact
    11521 ataacggtgt tttataatca ggcgtgaaaa cattaacacc caaacgaata tacccgcatt
    11581 gtaatgtgtt tctcgcatca gcagataagt actactacct ttaggaaacc agccacggag
    11641 ttccatcgcg gcataggtta ttgcaacgaa aagcagcgtt atccaatgta aataaatttg
    11701 agtttttgag aatcggttca tttttgtagc tcctgtaatt ttcacagtca agctatccca
    11761 cgaaccttaa aaattcctta tacagatgtg gttttaagaa tttgttattt aaaaaattga
    11821 tagtgcacat tgtaaccagc caatataagc aatgtcgtga gcacaggtat ctttctctgt
    11881 tggccgtatt gtgtatggaa tccgttattg gttaactcaa tgcgggttag cttattaata
    11941 tacagacacg tcatagtgat gagcgtgtct gtagctagct tgcgttcagt ggcgtttgtg
    12001 gatggaccag tggcagttat gatgacttac taaagcggca tcgaggcgtt atcatgagaa
    12061 tactcaatga gacatcattt cctcgaccac ttcttcgcta ctcaactgat atggataata
    12121 cgttggccag ttttccattt catttaataa agattgctgt gaatcattac ccataaaaat
    12181 gtgaaaatga gatgattttc gtggtgcaat tatatgatcg ctaaattgta tatatttagg
    12241 ggctttgctt tcaggatctt tacattcgaa taagtaacga acgcctttct tg
//
LOCUS       DAPGOF010000146        12178 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.147, whole genome shotgun sequence.
ACCESSION   DAPGOF010000146 DAPGOF010000000
VERSION     DAPGOF010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12178
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.147"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            5..835
                     /locus_tag="RHV13_003594"
     CDS             5..835
                     /locus_tag="RHV13_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418745.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfZ/YjiC family protein"
                     /protein_id="HDV1243086.1"
                     /translation="MSMPLSNALQSQIITDAHFLHHPIVDSEFTRKLKYARMDSENIY
                     LPPLTRGNNHNYDGKSVVEIRKLDISKEPWPFNYVTGACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHATTELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIEYISTLPASQAKRWTNEQPRIDIYIDQIMTVTGVANSSGFALAALLNANIELGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(976..1749)
                     /gene="uxuR"
                     /locus_tag="RHV13_003595"
     CDS             complement(976..1749)
                     /gene="uxuR"
                     /locus_tag="RHV13_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HDV1243087.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDNSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1964..3424)
                     /locus_tag="RHV13_003596"
     CDS             complement(1964..3424)
                     /locus_tag="RHV13_003596"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HDV1243088.1"
                     /translation="MTTIVDSNLPVARPSWEHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3505..4689)
                     /gene="uxuA"
                     /locus_tag="RHV13_003597"
     CDS             complement(3505..4689)
                     /gene="uxuA"
                     /locus_tag="RHV13_003597"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDV1243089.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAV
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5141..5233
                     /locus_tag="RHV13_003598"
                     /pseudo
     CDS             5141..5233
                     /locus_tag="RHV13_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5476..6378)
                     /gene="fimH"
                     /locus_tag="RHV13_003599"
     CDS             complement(5476..6378)
                     /gene="fimH"
                     /locus_tag="RHV13_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HDV1243090.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGSVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTVDAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(6398..6901)
                     /gene="fimG"
                     /locus_tag="RHV13_003600"
     CDS             complement(6398..6901)
                     /gene="fimG"
                     /locus_tag="RHV13_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HDV1243091.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(6914..7444)
                     /gene="fimF"
                     /locus_tag="RHV13_003601"
     CDS             complement(6914..7444)
                     /gene="fimF"
                     /locus_tag="RHV13_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HDV1243092.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(7454..10090)
                     /gene="fimD"
                     /locus_tag="RHV13_003602"
     CDS             complement(7454..10090)
                     /gene="fimD"
                     /locus_tag="RHV13_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HDV1243093.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(10157..10882)
                     /gene="fimC"
                     /locus_tag="RHV13_003603"
     CDS             complement(10157..10882)
                     /gene="fimC"
                     /locus_tag="RHV13_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HDV1243094.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPIGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(10919..11458)
                     /locus_tag="RHV13_003604"
     CDS             complement(10919..11458)
                     /locus_tag="RHV13_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV1243095.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(11523..12071)
                     /gene="fimA"
                     /locus_tag="RHV13_003605"
     CDS             complement(11523..12071)
                     /gene="fimA"
                     /locus_tag="RHV13_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HDV1243096.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
BASE COUNT         3005 a         3183 c         2901 g         3089 t
ORIGIN      
        1 tattatgtct atgccattaa gcaatgccct tcagagtcaa attattacag atgctcactt
       61 ccttcatcac ccaatagtcg acagtgagtt tacacgtaaa ttaaaatatg cacgcatgga
      121 ttcggaaaac atctatcttc cgcctcttac tcgtggcaat aaccataatt acgatggaaa
      181 aagtgttgtc gaaattagaa aacttgatat tagtaaagag ccctggccat ttaattatgt
      241 gacaggagca tgcagagaat ccgatggtat tacaacaaca ggacgtatgc tctatcggaa
      301 cttgaaaatc acctcagctt tagacgaaat atatggtggc atatgtaaaa aggcgcatgc
      361 taccacagag ttagcagaag gtttacggct aaatttattt atgaagagcc ctttcgatcc
      421 tgttgaggac tatacagttc atgaaataac attaggccct ggttgcaatg ttcctggtta
      481 tgccggaacc acgatagaat atatttcaac ccttccagct tctcaggcaa aaagatggac
      541 caatgagcag ccaaggattg atatctatat tgatcagata atgacggtaa caggggtagc
      601 aaattcttcc ggattcgccc ttgccgcatt acttaatgct aatattgagt tgggtaatga
      661 cccaataatt ggtatagaag cctatcccgg taccgcggag atccatgcaa aaatgggata
      721 taaagttatc ccgggtgatg aaaatgcacc actcaaacgt atgactctgc aaccgtcatc
      781 tcttccagaa ctgtttgaat taaaaaatgg tgaatggaat tatattggta aataactacc
      841 aggaagaatg agtactaaca ttcaggttat acagatagct aaacaatacc ctgcaattta
      901 caaagtaata ctcattgcga cttcttaatt taagaaagcg caaggaacgt ttacccttgc
      961 gcttattata ataagtcagg cgtcgacttt atccagcggc catgaatcaa acagatagcc
     1021 atcaaaataa atatcgtcaa cgttcgagaa ttccagcaga cgttgcttaa cgttttccag
     1081 atgctgccac attgccagct tcgccgctcg ggcatctttt ttgattaacg cggcgaggat
     1141 ctgtttgtga tcgcccaacc actctttgcg atacaggctg tcatccagat ggctgtgcaa
     1201 ctggatccac attggattgt tttcccgcca ctgccaggac tgacggaaca gctccaccag
     1261 catgctgtta tgcgttgctt cggcaatggc gagatggaac tgcatatcac cgctttcgct
     1321 gctacccggc gcgctggaag ccagttcacg ctcttccagt tgcaatgcct gacgcatttt
     1381 gacgatatct tcgcgggtag cctgcagggc ggcaaactcg gcgatgttac tttccagtaa
     1441 ctgacgcgcc tgtaacagct caaaaggacc cgcatcattg cagacgttgg catccggact
     1501 gtctgtgttg tggctgcctg agttatcaag aacatagata ccggcacccc ggcgtacttc
     1561 caccagccct ttgatctcca gcatgatcag cgcttcacgt accaccgtcc gcgtgacatc
     1621 aagcatttct gcaatttcac gctccggcgg cagccgttcg ccagggttgt acggcgtctt
     1681 tatgatcaga tcgcggatca tcgccccgac ttcctggtaa ggtctttgcg cagaggtggc
     1741 agatttcatg gtgttgtgtc cgttaggtgc ggttaatcaa atttttgccg gatgcggcgt
     1801 gaacgcctta tccggcctac aacgggcaat tttacggccc ctgttaatct caaactatac
     1861 cattagcact ggaagttggc acgtacaggt gggcaaaaaa acgaaaactg gtcaaccaaa
     1921 tatcagtttg attgaccagt aagtctgtag ggtaatcagt tagttacagc gcagccacac
     1981 actcgcgcgc accgcgttcg cacagctgct gatatgccgc cgtcaccgcg ccaacaaagt
     2041 cggcattctg cggcagatca tcggcaaaaa taccgctcag gcctagcagc gctttcacgc
     2101 ggtctgcgcc ctgatactgc gcgttaatct tctggaactc ggccagcatc gggtcgacca
     2161 cgtcaatggc attaccctgc tcatccacgc cctgggtgta acgcatccag ccagccacgc
     2221 ccagcgccag gtgacgccag ctgccgccgt tttgcaggtg cagacgcacc gggtccagca
     2281 gacgctgcgg taacttctgg ctgccgtcca tcgcaatctg ccaggtacgg tgacgcagag
     2341 acgggttgct gaaacgctcg atcagcagcg tcgcataggc gttcaggtcc gtaccttccg
     2401 gcatcgacag cgttggcgct tgttcctgca tcatcagggc aaaggccgct ttgcgataat
     2461 ccgggttagt catggtgtcg gcaatagttt catagccgcc gaggtaaccg aggtacgcca
     2521 ggaaagagtg gctaccgttc agcatacgca gcttcatcat ttcgaacggc acaacgtctg
     2581 caacgaactg tgcgcccact ttatcccagt ccgggcgacc attaacgaaa ttatcttcaa
     2641 tcacccactg acggaacggt tcgcaggcaa tggcgcacgg atcgtaaacg cccagctggt
     2701 cagcaatttc ctgtaaggtt tctggcgtcg ccgccggaac gatgcggtca accatggtgc
     2761 acgggaaggt gacgttctct tcaatccatg ccgccagctg cgggtcacgc gcctgagcca
     2821 gtcccagtac cgcgaccttc gccacatggc cgttttcacg cacgttatcg caggacatca
     2881 ccgtaaacgc tttcaaccct ttttcacgac gcagccgcag ggcttcgacg atgtaaccaa
     2941 tcgcggactt cggcgcagtc gggttttcca gatcgtgctt gatcagtggg ttattgagat
     3001 ccagctgacc gcttgccgca tcagcgcagt agcctttttc cgtgactgtc agagagacaa
     3061 tcgccgtctg cggacgcgcc atcgcattga gaataccttc gcagccgtcg atttccggat
     3121 gcagcgcttc tttcatcgaa ccgataattt tcagctcggt gctttccgcg cctttttccg
     3181 ccacggtgta cagcagttgc tgttttttca ggttttcgat cagcacgcgg tcgttgcctg
     3241 gcatcaggtt aacttcgcag ataccccagt cgctgtcggt gctttccagc aggtgatggg
     3301 tatacagcgc ctggtgcgcg cggtgaaacg ccccgcaacc gagatgcaca atgcgtgatt
     3361 ccagacgaga atgctcccat gacgggcggg caaccggcag attgctgtca acaatagtag
     3421 tcatcgtaaa ctccagaatc cgtcttcaga gagtgacggc aaggaagaag gaaccggcac
     3481 ggcgcgcagc catgccggtg ggtattaacg gctaaagaaa gcgcgctgga tcgccagttc
     3541 gacaccgcga acttcggcca ggcctttcag acgaccaatt gcggagtaac ctgggttggt
     3601 tttcttcttc aggtcgtcca gcatctgatg accgtggtcc ggacgcatcg ggatcaggtc
     3661 ttctttgcct tccgctttac gacggtgttc ttcttcaaca atcgctttca ccacttcgta
     3721 catatcaacg tcaccgttca ggtgcgccgc ttcgtggaag gttttcgggt tatcttcacg
     3781 catggtggag cgcagatggg tgaagtaaat acgcggaccg aactgcttga tcatatcaac
     3841 cagatcgttg tcagcacgca cgccgtagga accggtgcac atggtgaaac cgttcgccat
     3901 gctgtttacg gtatcaacca tccactgcat atcttcaatg gtggaaacaa tgcgcggcag
     3961 gccaaggatc gggcgcggcg gatcgtccgg gtgaaccgcc atacgcacgc caacttcttc
     4021 agcaactgga ataatcgctt tcaggaagac ggcaaagttt tcgcgcagct tcgctttatc
     4081 gatatctttg tacagctcca ggtgcttacg gaactggtcg agggtatacc cttcttccgc
     4141 gcccggcaga ccggcaatga tgttacgcgt cagacgcgct ttgtcttcgt cgctcatagt
     4201 ggcgaagcga acagcagcct gagcaatttc ttcttcggtg taatccgctt ccgcgcctgg
     4261 acgtttcagg atatgcattt cgaatgcagc gaattcgatc tggtcgaagc gcagagcttt
     4321 ggagccgtct ggcagcacgt attcgaggtc agtacgggtc cagtcgagca ccggcatgaa
     4381 gttgtagcac acggtgcgaa tgccgcactg cgccaggttg cgcagagtct gctgatagtt
     4441 agcgatccac tgctcatagt tgccagtgtg ggttttgata tcttcgtgaa ttggtacgct
     4501 ttctacgaca gaccacacta ggcctgcgtc ttcaacgatc gccttgcgtt tgaggatctc
     4561 ttctacggac catacttcgc cgttcgggat atggtgcagc gcggtaacca cgccagttgc
     4621 gcccgcctga cggacatcag ctaaagaaac cggatcgttt gggccgtacc agcgccaggt
     4681 ctgttccatg atttttcctc tttcacatag tgggttccac agcaaagaga cgcggaaccc
     4741 tggagttgtt taggtatccg gatgaacaac cagaccgcag ccgtggcgtt gcatcggatg
     4801 agatggcgta taagttctac cttttggtaa gacatgtcaa cccgaattct gaaattggtt
     4861 aaccacatca caagaatttc actctcatgg ttgttgctgc aaattgcagc aaagggtaat
     4921 cgatggttat tgatcagagg atgtgacatt catcgcaaca atggttgacc aatttacata
     4981 acatgtcgcc caaaataaca acggttcaac caggtaaggt aaaacgttat gcatgtgctt
     5041 aacattctct gggtggtatt cggcattggc ctgatgctgg tactgacaaa gaccgcaggt
     5101 tcagacacat tcgctgcatt agcaatgcct cttagataat tttgacctgt cagtaaaaga
     5161 cacgttgaaa acctggggtc tgctggagct ggtcaactcc gtggttgggc tgattattag
     5221 caaggtagcg taaaagaaca ccagagcctg ccaatggcag gctctgactg atgacaaagt
     5281 ctaaaaatgt gcccggacag tcccctcgcc cctccgggga gagggttagg gtgaggggaa
     5341 cagaccagca aagtgcgctt tgtttatgcc agatgcgacg ctgacgcgtc ttatctggcc
     5401 tacaaagggc taacgtgcag gtttttagct tcaggtaata ttgcgtaact gcattagcaa
     5461 tgccctgtga tttctttatt gataaacaaa agtcacgcca ataatcgatt gcacattccc
     5521 tgcagtcacc tgccctccgg tacgtgcgta atttgccgtt aatcccagac ttaccgccga
     5581 agtccctact gctcctaacg ataccgtgtt attcgctgga ataatcgtac cgttgcgcgt
     5641 caactgtacg ccgacgccct gcgctggtga aaacgacgcg gtattggtga aaatcgagtt
     5701 gcccgcatct acggttgtgc cggagaggta ataccccaga ttttggcttt tcgcacaata
     5761 aacggtaaga ggaattggca ctgaaccagg gtagtccggc agagtaacgg tgacatcacg
     5821 agcagaaaca tcgcagccgc cagtaggcac caccacatca ttattggcgt aaatattcca
     5881 cacaaactga aaatcatcgc tgttatagtt gttggtctgt cgcaaaataa gcacggcaat
     5941 taatgagcca gctttaatcg ccaccccgcc cgcactgctc acaggcgtca aataaagcgc
     6001 caccggccac ggcttatccg ttctcgaatt ataaacaacg cgcggcgttt cgctggtggt
     6061 aggaaatgga tagctactgc cactatattt tacggtcccg gaaaaattag ataacacgct
     6121 gccataagcc gagcctcgtt gcagtgtgac atagtctgta atggtttccg gataatcgtt
     6181 atggcaaaag atttgcgtcg aaagatccac gaccaggttt tgccccacat tcacggcggg
     6241 cgcaaggttt acataaacat tggcgctgcc accgccaata gggatagcgg taccattggc
     6301 ggttttacag gcgaatgacc aggcatttac cgaccagccc atcagcagta cagcaaacag
     6361 ggtaataact cgtttcatta caatcatctc ttcgggttca gctgtaggta taggtgatgc
     6421 taatcactgc ctgaatggtt ccctgagtgg ctccgccatt taccgtcaat gctctgacct
     6481 gtaacgggaa gtgcgctgat tgtgaggaat catccacctg aactgttttg gttgcgccag
     6541 tattcaatgt gttgccactg tcatcctgta gctctaactg gatgttttgc gcggtcccct
     6601 ggtttttata atatccggta ctgtcggctg ccccgctgaa gctggcggtg accctcgacg
     6661 ttcccaccgg acaattagtc aactcaagcg caacatcatg ccaggccgat gccgccccgg
     6721 cagacatcag actgaaagaa taaagatcgc cgagatcaac cgtggcattg gtggtggaaa
     6781 ccgtacacgg tttggcgacg accttaccgt tcaccgtgat ggtgacatcg gctgcctgta
     6841 tcgtcgcact tgcgagcgcc aatattgccg ccaataaata cccacgtttg cgccatttca
     6901 tgagcatctc cagttactga tattcaagag tgaaggtagc cgtggcattg atatgccccg
     6961 cagtgacagg cacctgtgtc gccattagcc gggcgtaaaa attcaacgta tttggtttac
     7021 ccggcgtcag ggtcgtccac gaaatcgcgg acgatggagc attaaggggt atttgatttt
     7081 gctgctcatt cagaagctgt attcccagtc ccgaagccgc tgacaccgta ttttcaagtg
     7141 caagcagatt ggcattgtgg ctatctgcaa cgccggtaaa cccaactttt acggcagaaa
     7201 cggcattacc acagggtgac agcaaaatac gaaatggaac aacaggagtc gtcgcgccaa
     7261 tgttgttaaa ttgcttcgcc gcgttttcca tcagatcaac agtaaaattg gttgattcag
     7321 cggccacact acagccgtta tctctgacat agccgcggat agtaatcgtg ctatccgcag
     7381 ccaaagcgtg actcaccgcc agccacaaaa aagcgcacag aagataaaaa ggtttgtttc
     7441 tcatcacgcc cccttaacga cattcagctg atagctgggt taataactgc tgctgactct
     7501 ctggtggcag ttgataattg gcgacacagt gagcattttc ctcttctccc catttcacct
     7561 gaacttttcc cgctaaaggc attccgctga ggtaaacctg accattatcc gcaacaatgc
     7621 cgctactctg gctactctct gatgtcacca tcgccccaaa cggcagcggc ttattattgt
     7681 gggttagcgt catgagcagt tttatcccaa cgcgcgcttt aaactctgct cgcacgatcg
     7741 ccccacgagt gggaacaacg ttagcgaccg cgttatctaa atcgacgtta tcagccaggg
     7801 tattggtatc cagcgccact ctgttttccc tatattcagt ggcataaggc agcacggcat
     7861 aaccgcgcca gtcggtacgc acccccgtct ggttttcgac ttttgcatct tttgcgccag
     7921 gcgctttaac aagcaccacc gtatcgttta acggctgccc cagcgttacg ccattggcat
     7981 gagccaatac cccaccgctg actccgtaat agagctgctt aatatcatcg ctatggctgt
     8041 aaccgatatt ggcattgccg taaccaccgc gataattcag cgtggcgtag cctgtgcttc
     8101 cgctattacc atcgcctccc ccggcatagc cggtttgcac gctatagctg aggttgttgt
     8161 cttccagcaa cgtaccgtat acaccagcca gattggtcat ccgaccgttg agatcgtgtg
     8221 acatgctgta gctggcactg gcatgtcgcc actgagattt actgtcagaa cgcagccagt
     8281 ggctgaaagg aatattgacg ttaagcgcta acatctgatc acgtcctttt tgccaggcgt
     8341 ttttcgtcag gctatagctg agcgtccagt tgatatcttc gaacgcagta tttaatccag
     8401 cctggaattg ctcatcgaca ttactcgttc cccaataagt ttgatggcta ccactcaaat
     8461 acagtgttga tgtgcgcccg agttgctgag taacggtgag ctgtaatttc ccgcgtttgt
     8521 tataagcgag gttgtaatag tcggtgaatt tcggcttaac ctgaataact ccatcctgtg
     8581 tttcgatgtt gtagccattc attcgactgt atgttgtatc agcgaaatta aaatatccgc
     8641 tggtcgaata acggtaaccc actaactgaa tattcgtgcc tgattcattg agcgatttgt
     8701 tatagagaaa acgcaccgat tgtccgtcat gctgactgtc atcgggaagt gtggaattag
     8761 cctgcgtcat atccacagac agagcgccca gtgcccccat gtttttcccg ataccgaaat
     8821 taaaagcacg ataacgatcc gccagttgcg ttccaccata tattgtccag ccagccggaa
     8881 ggccgtggag taatgtactc tggaaaaagc ggggtttttc ctgctgcgca tttccactac
     8941 ggtattctcc tgccgtaatg gaataacgag tatgcccttc acgttgcaaa agcgggactg
     9001 acgaataggg tacggtaaaa atctgcgtgc tgccgtcagc ctctttgatc gttacctgca
     9061 agtcaccact attacctgcg gcatagatat cgttgatggt aaaaggcccc ggtggcaccg
     9121 tactattata aatgtcatac ccattttgtt taatagtgac ctgtgcagta ccacgagcaa
     9181 taccgtggat caccggggca aatcctcttt ggctatcggg taacatattg tcatctgagg
     9241 ccaattgtgc gccgcgaaag ttaataccat cgaaaatatc gccctgagta taaccatcac
     9301 ccagcgtcag ccgggaacgt aacggtatta tgtctcgctc aagccaggta ttgatatgct
     9361 gccatttatt tttgctacct gatgatctgt cgctactgtt ataactccag gtggtattgt
     9421 cgcgtaaacg ccacgcacca atatttaacc cactctgtag gtttaaatat gcataatggc
     9481 tgttaccccc aatccgattc tgtacactat ttccgctgaa attataattg agcaatccgg
     9541 cattaatacc gggatcccat aactcaggag gaatataacc acgcgcgcga ttactcataa
     9601 atgcctgagg gatcgtcagg ttcagtcgct gctgaccaac atccagatgc gcagtagcgt
     9661 cctggaccat tgtggttaat ggcacacagg catcatccgc cagcagattc ataccggcga
     9721 cagaagccgt attcagcccc atactggcga gttgcgcgcg tgtcaggcag ggaacaatcc
     9781 cttgttcact gtcgcccgta ttaaatgtga catcacgcgt tgccatataa ccattattca
     9841 aatagatatc gacgcgatac gtccctggcg gtaattcttg cccattttca aaacgcgata
     9901 aatcggccac agcctgggga tcatccgcta aaaaacgcgg gttaaaatag agttcggcag
     9961 atgacaaagg tgcctgtgcg gcaaaagcac aggcaacaaa gagccggaca aaaaaaccag
    10021 ccaaacgatg cttacgaata tgcaagcatt gtgtgtttcg ctggtaaagt cttaaattca
    10081 gatatgacat tattcctccg gtttaaagtc acccggcagt caattctttt tattcaggcg
    10141 aaaaattccc cctgcgttat tccattacgc ccgtcatttt gggggtaagt gcaccataat
    10201 catttattgt tcggtaagta atattgcttc ccgcatcaga aggcaattta accgtgcttt
    10261 cgcctattgg aggcaccaag gcattttcaa gaacccgggt tccggcattc aactctgtta
    10321 ccgtcaggta atagggtgtc gggttaatca gcgtcagaga attcgcgcta cgacgaaatc
    10381 ttaatttttc tgcggcctga tcgggtggca acgctaattt agccgggcga tagtacagtt
    10441 taatgcggct gataattgcg agctgtagcg tattctcagt caattttgat ttatccattg
    10501 acggaatcgc tttaacattc atccagaata aactttcccg atcctgtggc aattggttat
    10561 ttgttgcatc aagaatacgc aaggtattct ctttttttcc cttcatcgca aacagaggag
    10621 gcgtcacgat aaaacgacca tcctttacac catcggcatt ttccacccat gattgaatta
    10681 aataggtact attttcatca ttatttgtca cggcaagttg cacttgtttt tgccctgccg
    10741 gataaattac gcgagtcgca cctaaggcca ctcccgcttc agcgcgtccg gcaaccatca
    10801 ttgccataaa catcaggata cctgccagca agcagaatgt tatttcctgc gatttcctta
    10861 cattgacgtt tttattactc actgtcctgt tcctgttatc acattatctg ctgaacaatt
    10921 actgataggt taaagagaac caggcctggg cattggcgat gccgccagta acccgacgcc
    10981 cggtagcacg atatttggcg atgaaatgta gcgaagtaga gcctgagtaa agccgtttcc
    11041 agtttgctgg aggacgatta atcggtacga ggtttccttc atcatcaaac aacgctacgc
    11101 caatattggt ggctatccct ggcccctctc ccacggaaag cacatccgga tttttaccat
    11161 ccgcgacacc gtgaaacgcc acccccacac gttcactcac caccgtgcta cattcccgta
    11221 aatgaataac aaaaggcacc ggtgcgctat cttcccctgc cgcatgaaac cggttactgc
    11281 tgatttgccc catattgacc gtcatttgtt tatcaccggc ttcaatccgg caagtttccg
    11341 caataatgac gccctgaaat tgcatatttc cgccgggcag cgtggtattc catttatttc
    11401 cggccagagc aaacattggc agcaacgacg ctaacagaaa taatctcttc cttttcatcg
    11461 ttatttttat cgcacaaggg tgggcatccc tgcccgtaat gacgtccttg aacctgggta
    11521 ggttattgat actgaacctt gaaggttgca tccgcattag ctgcacccgg ggttgcctcg
    11581 ccgattgcat aataacgcgc ctggaacgga atggtgttgg taccgttatt cagggttgtt
    11641 tgtgcactaa atgtcgcacc gtccagcgtc agagcattgc ctgttctgtc caggatctgc
    11701 acgccaacgt ttgttgcact acctgcagca gaactctgca gagccagtac atcagtacgc
    11761 gtagcatcaa ttgccgtacc taagaaggca acagcggctt ttgtggcaac agtggtatcg
    11821 caatcattca actgaatgtt aaaaccaacg gcagagctgg ttgctccagc ctgcttcagg
    11881 ctagcggtac gaacctgtcc caactgaacg gtttgatcaa cagagcctgc atcaactgcg
    11941 caagcggcgt taacaacttc ccctttaaag tgaacggtcc caccatttac cgtcgtagta
    12001 tcggccagag ccgctgcgga actgagggac agagccgaca gaacaacgat tgccagagtt
    12061 ttaattttca tgctgctttc ctttaaaaaa actatttcta aatcgacatg ggcagtcgtt
    12121 ctgtacactt tgttttgtca acgagtttga ctgccaacac tgcacagttt tcccccaa
//
LOCUS       DAPGOF010000147        12167 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.148, whole genome shotgun sequence.
ACCESSION   DAPGOF010000147 DAPGOF010000000
VERSION     DAPGOF010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.148"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            356..1744
                     /gene="narU"
                     /locus_tag="RHV13_003606"
     CDS             356..1744
                     /gene="narU"
                     /locus_tag="RHV13_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HDV1243097.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAR
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPIFAFLGVNGVPQADGSVISLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASITDQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1839..5579
                     /gene="narZ"
                     /locus_tag="RHV13_003607"
     CDS             1839..5579
                     /gene="narZ"
                     /locus_tag="RHV13_003607"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HDV1243098.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELTLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVREMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5576..7120
                     /gene="narH"
                     /locus_tag="RHV13_003608"
     CDS             5576..7120
                     /gene="narH"
                     /locus_tag="RHV13_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HDV1243099.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPKGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVLQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7120..7815
                     /gene="narW"
                     /locus_tag="RHV13_003609"
     CDS             7120..7815
                     /gene="narW"
                     /locus_tag="RHV13_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HDV1243100.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTRNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWSSLFDALLQLAGS
                     SLSSDSVTKQIRSEERDDTPQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7812..8492
                     /gene="narI"
                     /locus_tag="RHV13_003610"
     CDS             7812..8492
                     /gene="narI"
                     /locus_tag="RHV13_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HDV1243101.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLVQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8571..9464
                     /locus_tag="RHV13_003611"
     CDS             8571..9464
                     /locus_tag="RHV13_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HDV1243102.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEVQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTVWQTSLAGK
                     HRVTIEKHHDGYRISLEQGTPGFEPPLEGETRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGKVLRVKGHQGRALGRDGVIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEL"
     gene            complement(9560..10405)
                     /gene="nhoA"
                     /locus_tag="RHV13_003612"
     CDS             complement(9560..10405)
                     /gene="nhoA"
                     /locus_tag="RHV13_003612"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HDV1243103.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREMQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEEEKWISDVGFGGQTLTAPIRLVPDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10578..11147
                     /locus_tag="RHV13_003613"
     CDS             10578..11147
                     /locus_tag="RHV13_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDV1243104.1"
                     /translation="MSRFIPVELHHASRLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQEKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11151..11378)
                     /gene="pptA"
                     /locus_tag="RHV13_003614"
     CDS             complement(11151..11378)
                     /gene="pptA"
                     /locus_tag="RHV13_003614"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HDV1243105.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
     gene            complement(11689..>12167)
                     /locus_tag="RHV13_003615"
     CDS             complement(11689..>12167)
                     /locus_tag="RHV13_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HDV1243106.1"
                     /translation="DSYAISEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAV
                     SFRSIIAEQKKEPEMTVRYYISSVDLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDC
                     KIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
BASE COUNT         2888 a         3151 c         3136 g         2992 t
ORIGIN      
        1 gatcgttttc ctccagctca tggccttttt agtgaataaa ttatcactaa ttctataagt
       61 gtctggtctt gttaagaata tagttaatat acaacccttt ggattcatca tctgaagggt
      121 tgaatcatga catgcgttca aagatcaccc cccgtttaat ggctagcttg tcagttacat
      181 ttagtaacac ataactggct gcttaatccc cattctgccc ccgcggttac gctttcttat
      241 ctattctttc aatcagcact tcacccaatt catccttaat tctaataatc ggctattgcg
      301 ccttacctcc tgttttgtct tttttcatta ccaatgtgtg catgtgagga acaatatggc
      361 actgcaaaat gagaaaaata gtcgttatct tttacgcgac tggaaaccag aaaatccggc
      421 cttctgggaa aataaaggaa aacatattgc tcgaagaaac ctctggatat cagtcagttg
      481 tctacttctt gccttctgtg tctggatgct atttagcgca gttaccgtta atctcaataa
      541 aatcggtttt aattttacta ccgatcaact ctttttatta accgcattac cctccgtttc
      601 tggcgcatta ttgcgtgttc cctactcctt tatggtacct atattcggtg gacgccgatg
      661 gacggttttt agtactgcaa tcctgattat tccttgcgtc tggcttggaa ttgccgtgca
      721 aaatccgaat actccttttg ggatctttat cgttatcgct ttgctatgcg gttttgcagg
      781 tgcgaacttt gcttcgagca tgggcaatat cagtttcttc tttccaaaag ccaggcaagg
      841 gagcgctctt gggattaatg gcggattagg aaacttaggt gtaagtgtga tgcagctggt
      901 tgcaccgctg gtcatttttg tacccatatt tgcctttctc ggcgtcaatg gcgtaccgca
      961 ggccgacggt tcggtgattt cgctggcgaa tgccgcatgg atttgggtgc cgctactggc
     1021 gattgctacg atcgccgcct ggtcagggat gaatgatatt gccagttccc gcgcgtcaat
     1081 taccgaccag ctccctgtgt tacaacgtct gcatctctgg ctgctgagcc tgctttacct
     1141 tgccaccttc ggttcgttta tcggtttttc tgcgggtttt gccatgctgg caaaaaccca
     1201 gttcccggat gtgaatattc tgcgcctggc gttctttggc ccatttatcg gtgccatcgc
     1261 gcggtcggtg ggtggtgcta tttccgataa attcggcggc gtgcgagtga cgttgatcaa
     1321 ctttattttt atggcgattt tcagcgccct gctgttcctt accttaccgg gcacaggctc
     1381 cggtaatttc atcgcctttt acgccgtatt tatggggctg tttctgaccg cgggtctggg
     1441 aagtggttct actttccaga tgatcgccgt catctttcgc cagataacca tttatcgggt
     1501 gaagatgaaa ggcggtagtg atgagcaagc tcagagagaa gccgtcaccg aaacggcggc
     1561 ggctctgggc tttatctcag ccattggcgc agtgggcggc ttttttattc cgcaggcgtt
     1621 tggcatgtcg ctcaatatga ccggctctcc agtgggcgcg atgaaagtgt ttttaatctt
     1681 ctacatcgtt tgtgtgctac tgacctggct ggtttatggt cggcggaaat tcagccaaaa
     1741 ataagagatc gatgcctgac acgtcttatc aggctacaac cgtgtcgcat ccgacaaggc
     1801 atcagatccc aattcattcc tggagcagga gtcatgtcat gagtaaactt ttggatcgct
     1861 ttcgctactt caaacaaaag ggcgaaacct ttgccgatgg tcacggacag gtgatgcata
     1921 gcaaccgcga ctgggaggac agctatcgcc agcgttggca gttcgacaaa atcgtgcgtt
     1981 ccacccacgg tgttaactgt acaggctcct gtagctggaa aatctacgtt aaaaatggtc
     2041 tggtgacctg ggaaatccaa cagaccgact acccgcgcac tcgccctgac ctgcccaatc
     2101 atgaacctcg cggctgcccg cgtggcgcaa gttactcctg gtatctttac agcgctaacc
     2161 gcctgaaata cccgctcatt cgtaaacgac tgattgaact gtggcgcgaa gccctcaaac
     2221 aacacagcga tccggtactg gcgtgggcat cgattatgaa cgatccgcaa aagtgcctga
     2281 gctacaaaca agtgcgtggg cgcggcgggt ttatccgctc caactggcag gaactaaacc
     2341 agctgattgc cgccgctaac gtctggacca tcaaaaccta cggcccggat cgcgttgccg
     2401 gtttctcgcc gatcccggcg atgtcgatgg tttcttacgc cgccggaaca cgttatctgt
     2461 cactattagg tggcacctgt ttaagcttct acgactggta ttgcgacctg ccgcccgcct
     2521 cgccgatgac ctggggcgag caaaccgacg taccggaatc tgccgactgg tataactcca
     2581 gttacatcat cgcctggggg tctaacgtac cgcagacacg tacgccggac gcccacttct
     2641 ttaccgaagt acgctacaaa ggcaccaaaa ctatcgccat tacccctgac tactctgaag
     2701 tggccaaatt gtgcgaccag tggctggcac caaaacaagg cactgatagc gccctggcga
     2761 tggcaatggg ccatgtgatt ttaaaagagt ttcatctcga taatcccagc gactacttta
     2821 tcaactactg ccgccgctat agcgacatgc cgatgctggt aatgctggag cctcgcgacg
     2881 atggtagcta cgttcccggg cgcatgatcc gtgcgtctga cctggtggat ggactgggcg
     2941 aaagcaacaa tccgcagtgg aaaaccgtag cagttaatac cgcaggtgaa ttggtagtgc
     3001 cgaacggttc gattggtttc cgctggggag aaaaaggcaa atggaatctg gaatccattg
     3061 ccgccggtac ggaaaccgaa ttgacgttaa ccctgctcgg tcaacatgac gctgttgcag
     3121 gcgtggcctt cccctacttt ggcggcattg aaaatccgca ttttcgcagc gtaaaacaca
     3181 atccggtgct ggtgcgccaa ttgcccgtta aaaacctgac gttagtcgat ggcaacacct
     3241 gtccggtggt cagcgtttat gatttggtac tggcgaatta cggcctcgat cgcgggctgg
     3301 aagatgaaaa cagcgcgaaa gattacgctg aaatcaaacc gtacacccca gcctggggtg
     3361 agcaaattac cggcgtaccg cgccagtata ttgaaaccat cgcccgcgaa tttgccgata
     3421 ccgcccataa aacgcatggg cgctcaatga ttatcctcgg tgctggcgtc aaccactggt
     3481 atcacatgga tatgaactac cgtgggatga tcaatatgct gatcttctgc ggttgtgtcg
     3541 ggcaaagcgg cggcggctgg gcgcactatg tcggtcagga aaaactgcgc ccacaaaccg
     3601 gctggttgcc gctggccttt gcgctcgact ggaaccgccc accgcgccag atgaacagta
     3661 cctcgttttt ctacaatcat tccagccaat ggcgctatga aaaagtctct gcgcaggagt
     3721 tactttcacc gctcgccgat gccagtaagt acagcggtca tctgattgat ttcaacgttc
     3781 gcgccgaacg tatgggctgg ctgccctctg cgccgcagct ggggcgtaac ccgctcggga
     3841 ttaaagctga agccgacaaa gcaggattat cccccacaga atttaccgcc caggcgctga
     3901 aatcgggcga tttacgtatg gcctgcgaac aaccagatag cggcagcaat catccgcgta
     3961 atttgtttgt ctggcgttct aacctgcttg gctcctccgg caaaggccac gagtatatgc
     4021 agaagtatct gctggggacc gaaagcggga ttcagggcga ggaactcggt gccagcgacg
     4081 ggatcaaacc ggaagaagtc gagtggcaaa ctgcggcgat tgaaggcaag ctcgacctgc
     4141 tggtgacgct cgacttccgc atgtccagca cctgcctgtt ctccgatatt gttctgccta
     4201 ccgccacctg gtacgaaaaa gacgatatga atacctcgga tatgcatccg tttattcatc
     4261 cactttctgc ggcagtcgat ccggcctggg agtcacgcag cgactgggaa atctacaaag
     4321 gtattgccaa agcattttcg caagtgtgcg tgggtcatct tggcaaagaa accgacgtgg
     4381 tattacaacc actgctgcac gactctccgg cagagctctc acagccgtgt gaagtcctcg
     4441 actggcgcaa aggcgaatgc gatctgattc caggtaaaac cgcacccaat attgtggcgg
     4501 tggagcgcga ctaccctgct acctatgaac gctttacctc gctcgggcca ttgatggaca
     4561 aacttggcaa cggcggtaaa gggatttcgt ggaatacgca ggatgaaatc gatttcctcg
     4621 gtaaactcaa ttacaccaag cgtgatggcc cagcgcaggg gcgtccgctg attgacaccg
     4681 ccattgacgc ttcagaagtg attctggcac tggcaccaga aacaaacggt catgttgcag
     4741 ttaaagcgtg gcaggcgctg ggcgagatca ccggacgcga acatacccat ctggcgctgc
     4801 acaaagagga cgagaagatt cgctttcgcg atattcaggc gcagccgcgt aaaattatct
     4861 ccagccccac atggtctggt ctggaaagcg atcacgtctc ctataatgcg ggatacacca
     4921 acgttcatga gttaattccg tggcgcacgc tgtcgggacg ccagcagctc tatcaggatc
     4981 atccgtggat gcgtgctttt ggtgaaagcc tggtggctta tcgcccgcct atcgacaccc
     5041 gtagcgtcag agagatgcgc cagatcccgc caaacggctt cccggaaaaa gcacttaact
     5101 tcctgacgcc gcaccagaaa tggggcattc actcaaccta cagtgaaaac ctgctaatgc
     5161 tgacgctctc tcgcggtgga ccgattgtct ggatcagcga aaccgatgcc cgtgaactaa
     5221 ccattgtcga taacgactgg gtggaagtgt ttaacgccaa tggcgcgctg acggcccgcg
     5281 cggtggtcag ccaacgtgta ccgccgggta tgaccatgat gtatcacgct caggaacgca
     5341 ttatgaatat tcctggttcg gaagtaactg gcatgcgcgg cggtattcat aactcggtca
     5401 cccgcgtttg tccgaaacca acgcatatga ttggcggtta cgcacagctg gcctggggct
     5461 ttaactacta cggcaccgtc ggttcgaacc gcgacgaatt catcatgatc cgcaagatga
     5521 aaaacgttaa ctggctggat gatgaaggtc gcgatcaggt acaggaggcg aaaaaatgaa
     5581 aatacgttcg caagtcggca tggtgcttaa tctcgataaa tgtatcggct gccatacctg
     5641 ttcggtgacc tgtaaaaacg tctggaccgg acgcgaaggt atggagtacg catggtttaa
     5701 caacgtcgaa accaaaccgg gcattggtta tccgaaaaac tgggaagatc aggaagagtg
     5761 gcaaggcggc tgggtgcgtg atgtgaatgg caagatacgc ccgcgtctgg gcagcaagat
     5821 gggtgtaata accaaaatct tcgccaaccc ggtggtgccg cagattgatg attactacga
     5881 acctttcacc ttcgactacg aacatttgca tagcgcaccg aaaggcaaac atatccctac
     5941 tgctcgcccg cgttcgctga ttgacggtaa gcggatggac aaagtgatct gggggccaaa
     6001 ctgggaagaa ctgctgggcg gcgagtttga aaaacgtgcc cgcgaccgca acttcgaggc
     6061 catgcaaaag gagatgtacg ggcagtttga aaacaccttc atgatgtacc tgccgcgcct
     6121 gtgcgaacac tgcctcaatc ccagttgcgt ggcgacctgc ccaagcggcg ctatctacaa
     6181 acgcgaagaa gacggcattg tgctgattga tcaggataaa tgccgtggct ggcgtttgtg
     6241 cattagcggt tgtccgtaca aaaaaatcta cttcaactgg aaaagcggca agtcagaaaa
     6301 atgtatcttc tgttatccgc gaattgagtc aggccaacca accgtatgct cagaaacctg
     6361 cgtgggtcgc atccggtatc tgggcgtgct gctttacgac gccgaccgca ttgaggaagc
     6421 ggcaagcacc gagcgcgaag ttgacctcta tgagcgccag tgcgaagtgt tcctcgatcc
     6481 acacgatccc tcagtgatcg aggaagccct gaaacaaggt attccacaaa acgtgattga
     6541 agctgcccag cgttcgccag tctacaaaat ggcgatggac tggaaactgg cgctaccgtt
     6601 gcaccctgaa taccgcaccc tgccgatggt ctggtacgtt cctccgctgt caccgattca
     6661 gtcttacgct gatgcgggcg gtttgccgaa aagcgaaggc gtgctacccg ccatcgaaag
     6721 cctgcgtatt ccggtgcaat atcttgccaa tatgttgagt gccggtgata ccggtccggt
     6781 actgcgggcg ctgaaacgga tgatggctat gcgccactat atgcgttcac aaaccgtgga
     6841 aggcgttact gatactcgtg ccatcgacga agtaggcctg agcgtcctcc aggtcgaaga
     6901 gatgtatcgc tatctcgcca ttgccaacta tgaagaccgt tttgtcattc caaccagtca
     6961 tcgggaaatg gcgggcgatg ccttcgctga acgcaacggc tgcggtttta cctttggcga
     7021 cggttgtcac ggctcggaca gcaaattcaa cctgttcaac agtagccgta tcgatgccat
     7081 caacatcacc gaagtgcgcg acaaagcgga gggcgaataa tgcagatcct caaagtgatc
     7141 ggtctgttga tggagtatcc ggatgagctg ttgtgggaat gtaaggagga cgcgctggcg
     7201 ttgatccgcc gcgacgcgcc gatgcttacg gatttcaccc gcaacctgct taacgcgccg
     7261 ctgctggata aacaggccga atggtgcgaa gtgtttgacc gcgggcgcac cacgtcgctg
     7321 ctgctgttcg aacatgttca tgccgagtcc cgcgatcgcg gccaggcaat ggtggacctg
     7381 ctggcggagt atgaaaaggt cggcctgcag ctggattgtc gggaactgcc cgattatcta
     7441 ccgctgtatc tggagtactt aagcgtactg cctgacgatc aggcgaaaga aggattactc
     7501 aatgttgcgc cgatcctcgc cctgcttggc ggacgcttaa aacagcgcga ggcaccctgg
     7561 tcttcgttgt ttgacgccct gctgcaactg gcaggaagct ccctttcaag tgacagtgtc
     7621 acaaaacaaa ttcgcagtga ggagcgtgat gacacccccc aggcgctgga tgcggtgtgg
     7681 gaagaggaac aggtgaagtt tattgaagat aacgccacgg cgtgtgacag ctcgccgctt
     7741 aatcaatatc agcgacgctt tagccaggat gtcgcgccgc aatatgtcga catcagtgcg
     7801 ggaggtggga aatgattcag tatctgaacg tcttttttta cgatatctac ccgtacattt
     7861 gtgcgacggt atttttcctc ggcagctggc tgcgctacga ctacgggcag tacacctggc
     7921 gcgcctcctc aagtcagatg ctcgataaac gcgggatggt gatatggtcg aatctgttcc
     7981 atattggcat tttggggatt ttcttcgggc acctgttcgg catgttaacg ccgcactgga
     8041 tgtacgcgtg gtttttgcca gtggcagcga aacaactaat ggcgatggtg ctcggcggta
     8101 tttgcggtgt gttgacgttg attggcggcg caggactgct gtggcgcagg ctgacaaatc
     8161 agcgtgtgcg tgccacttcc actacgccgg atatcatcat catgagcatt ttgctggtcc
     8221 agtgtctgtt ggggctaagc accataccgt tttcagcaca gtatcctgat ggtagcgaaa
     8281 tgatgaagct ggtgggttgg gcgcaaagca ttgtcacttt ccgtggtgga tcgtcagaga
     8341 tgctcaacgg cgtagcgttc gtcttccgcc tacatctggt gttgggaatg accatcttcc
     8401 tgctattccc gttcacccga ctggtgcatg tgtggagcgc gccgtttgag tactttactc
     8461 gtcgatatca aattgtgcgt tcgcggcgat aatttcaccc tggccggata gcccttcgct
     8521 ctccggccac ttgcaggcta tgatagttct ccagtcacag gagaacgtta atgaaaccgc
     8581 aggtttacca cgtcgatgct tttacctctc aaccatttcg cggcaattct gccggtgttg
     8641 ttttccccgc cgataatctt agcgaagtgc aaatgcagct tatcgcccgc gaattaggtc
     8701 attcggaaac tgcttttctg ctgcacagcg acgacagcga tgtgcgcatc cgctacttta
     8761 cgccaaccgt tgaggttccg atttgtggtc acgcgacagt agctgcgcac tatgtacgtg
     8821 cgaaggtgtt aggtttagga aattgcacgg tctggcaaac atcgctggca ggaaaacatc
     8881 gcgtgactat cgaaaagcac cacgatggtt atcgtatttc gctggaacaa ggtacgccgg
     8941 gctttgagcc accgctggaa ggtgaaacac gtgcggcgat tatcaacgcg cttcatctca
     9001 ctgaggacga tattctgcca ggcttgccaa ttcaggtggc aaccacagga cactcaaaag
     9061 tgatgatccc actgaaaccg gaagtggata tcgacgccct ttcgcctgac cttaatgcgc
     9121 tgaccgctat cagcaaacag attggctgca atggtttctt cccgttccag atccgcccag
     9181 gcaaaaacga aaccgatggt cgtatgttct cgcctgccat tggtattgtg gaagatccgg
     9241 tgactggcaa tgccaatggc ccgatgggtg catggttggt acatcacaac gtattgcccc
     9301 acgatggcaa ggtgctgcgt gttaaaggcc atcaggggcg cgcattgggg cgtgacggcg
     9361 tgattgaagt gacggtgaca attcgtgata accaaccgga aaaagtcacc atttcaggca
     9421 cagcggtgat tttgtttcat gccgaatggg caattgaact ctgaaaccgt tgtctgatgc
     9481 gcttcgcctg tcaggtctac aaccgggcta atgtatgccg gataaggcat ttacgccgca
     9541 tccggcaacc tgacataaat tattttcccg cctccgggtg cgtatcaaac gctgccatca
     9601 ccagcgctaa ctcatccacg gtaaagccat gtttcgcatc atccacgccc agaccaaact
     9661 gttcctgcat cacggcatat aacgacgcca catccggtag atttcgctgc tccaccgcgt
     9721 gcccattttc ataatgggta aaatgaaaat tggtcagtgt cagcttgccg ccgtccggca
     9781 aatggcggca catcagcaaa tgatggcgaa aatgcgactg tggccagtgc gccgaccaga
     9841 aattgcccat cacataatcg ctttgttgct gctcgcagag atcaaaacgg tacatcgact
     9901 gccagtgctg atgatgatta aactgcaaca cccaatcatc accctcctgc aacaaccgat
     9961 actctccgtg tggcgtggtc tgcacgagat cgggaactaa acgaatcggc gcggttagcg
    10021 tctgcccacc gaaaccgaca tcagaaatcc atttttcctc ttccagttcc accaacagca
    10081 aacgatgggt gcgcggcggt aatgctggcg gatttgataa cactacacgc cctaacaagc
    10141 tgcgaacgtt aaaccccagc tcgcgtaaca cccgctcaaa cacgccattc tgctcaaaac
    10201 agtaaccgcc acgacgggct atcaccagtt tctcttccag cgattgatca tcaagctgca
    10261 tttccctcgg cagcaaaacg tcgaggtttt caaacggaat ggtgcaattg tgtttcaggt
    10321 gcaatgcacg aagcgtatca atattgaccg cagcagctcc cgaccagtta atacgggcaa
    10381 aatagtgatt cagaatgggc gtcatcaggc gtttccctta gtggttttct caacttataa
    10441 cctggaatag acgccgcacc ggctattttg tgcgatcgcg gacatgcatc acgttcgcaa
    10501 atcgccaatc ccgttaattt cgttacttat tgcgttacgg gctacgctta aaaaaacctt
    10561 cttcgcgagg cgataccatg agccgattta tccccgtcga attacaccat gccagccgtc
    10621 tgttaaatca tggcccaact gtcatgatca ccagtttcga tgagcaatcc cagcggcgta
    10681 acattatggc tgcagcctgg tcaatgccgg tggagtttga acctccacgc gtggcgattg
    10741 tggtagataa atcgacatgg accagggagt tgattgaacg taacggtaag tttggcatcg
    10801 ttatcccggg cgttgcagca actaactgga cgtgggcggt gggaagtgtg tcgggtcgtg
    10861 aagaagataa atttaattgc tacggcattc cggtggtgaa aggcccggtg cttggtttgc
    10921 cgctggtcga ggaaaaatgt ctggcgtgga tggagtgccg attgctacct gcgacctccg
    10981 cgcaagaaaa atacgatacg ctatttggcg aagtggtatc ggcagcggca gacgcacgag
    11041 tatttgtcga aggccgctgg cagtttgatg atgataaact caatacacta catcatttag
    11101 gcgctggcac cttcgttacc agcggcaagc gagttacggc aggttaagca ttacatgctg
    11161 taaccaggtt tctttatcaa agcctccatt tggggcgcga tttcggcgtc ccagatagct
    11221 tgccatgatt ctggttgaat ctgttgtaga gcaatgctta tcgaactgtc tttactgttc
    11281 agatggcgaa taataacgtc ggtaatatct gcagcaagtg ctgctttttg ttgttcgtcc
    11341 agttcacgtg gaaaacattt aatgtctatg tgcggcatga ttgtgctcct ttggaaagat
    11401 gtcagtaaaa ttaacacacg ttgtgaatgt catgtgtccg gtgatgcttt tttacgacaa
    11461 taatgcctac ttgaatttgt acatatttgt actaccccgt tttaaataaa gcgccaacaa
    11521 aaacgttaac ttgcagaata tattcttcgc caacatgcca ttaagaaaac atcaagttcc
    11581 cggcttgcca atttattaat gcccattata gacgctgaaa caagccacgg acaaagaaga
    11641 tgccaaaatt accggcaatg cagaaaaact taacgaacca gggcaagatt acgaaagccc
    11701 gctccccgca aggactgacg ccagatagtt tctgtccatg gctgcttttc gcatcttacg
    11761 tcttaaccct gccttgaata ccttatcatt cgtcaaaata ttaatagcaa tgtgccgtat
    11821 ccctgaaaat aattctgctg catttcctct tcttatcttg cagtcgtctt cattcattac
    11881 cacgtccaga cgccagtgca gcttattctc cacgtgccag tggtttcgga ttgctgtggc
    11941 gaacttctct gcggttaaat caacagaact gatataatat ctgaccgtca tttctggctc
    12001 tttcttttgt tctgctatta tggaccgaaa ggagactgcc acgcataatt tctttagccc
    12061 tttccattca aacgtgaaat caataagttc atcagggaca tcgcaaacaa tatgaagacg
    12121 gatttcttct ctgccgtgac tcttttcact aattgcgtaa ctgtcat
//
LOCUS       DAPGOF010000148        12097 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.149, whole genome shotgun sequence.
ACCESSION   DAPGOF010000148 DAPGOF010000000
VERSION     DAPGOF010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12097
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.149"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            290..1120
                     /locus_tag="RHV13_003616"
     CDS             290..1120
                     /locus_tag="RHV13_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HDV1243107.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSEVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            1178..2305
                     /gene="efeO"
                     /locus_tag="RHV13_003617"
     CDS             1178..2305
                     /gene="efeO"
                     /locus_tag="RHV13_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HDV1243108.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDKYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            2311..3582
                     /gene="efeB"
                     /locus_tag="RHV13_003618"
     CDS             2311..3582
                     /gene="efeB"
                     /locus_tag="RHV13_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HDV1243109.1"
                     /translation="MQYKDENGVNGPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQELAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            4203..4991
                     /gene="phoH"
                     /locus_tag="RHV13_003619"
     CDS             4203..4991
                     /gene="phoH"
                     /locus_tag="RHV13_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HDV1243110.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVHSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            5573..6259
                     /locus_tag="RHV13_003620"
     CDS             5573..6259
                     /locus_tag="RHV13_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HDV1243111.1"
                     /translation="MSFMSFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHERADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPLH"
     gene            complement(6351..6438)
                     /locus_tag="RHV13_003621"
     tRNA            complement(6351..6438)
                     /locus_tag="RHV13_003621"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6402..6404),aa:Ser,seq:gga)
     gene            6672..7610
                     /gene="ghrA"
                     /locus_tag="RHV13_003622"
     CDS             6672..7610
                     /gene="ghrA"
                     /locus_tag="RHV13_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HDV1243112.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRMTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            7665..8402
                     /gene="ycdX"
                     /locus_tag="RHV13_003623"
     CDS             7665..8402
                     /gene="ycdX"
                     /locus_tag="RHV13_003623"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HDV1243113.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTLGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            8426..8980
                     /gene="ycdY"
                     /locus_tag="RHV13_003624"
     CDS             8426..8980
                     /gene="ycdY"
                     /locus_tag="RHV13_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HDV1243114.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLVPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            9082..9573
                     /locus_tag="RHV13_003625"
     CDS             9082..9573
                     /locus_tag="RHV13_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HDV1243115.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALTPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(9637..10470)
                     /gene="csgG"
                     /locus_tag="RHV13_003626"
     CDS             complement(9637..10470)
                     /gene="csgG"
                     /locus_tag="RHV13_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HDV1243116.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(10497..10913)
                     /gene="csgF"
                     /locus_tag="RHV13_003627"
     CDS             complement(10497..10913)
                     /gene="csgF"
                     /locus_tag="RHV13_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HDV1243117.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(10938..11327)
                     /gene="csgE"
                     /locus_tag="RHV13_003628"
     CDS             complement(10938..11327)
                     /gene="csgE"
                     /locus_tag="RHV13_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HDV1243118.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            complement(11332..11982)
                     /gene="csgD"
                     /locus_tag="RHV13_003629"
     CDS             complement(11332..11982)
                     /gene="csgD"
                     /locus_tag="RHV13_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HDV1243119.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
BASE COUNT         3020 a         2771 c         3105 g         3201 t
ORIGIN      
        1 aacgttcttt tgtgaaacga ctaactgaaa attcaaccct tcggggcacg ctgcagcgag
       61 tttgtcgcca ccaacattcg tacaagcttc atctgccggg cgaaaagtca gctaaatacc
      121 ttaaaataat cgcttttgtt tccggatttg ttgtcctcgt ttttacattc caacatcatt
      181 ttataaacat tccgcttgta tttttcttta ccgtaatgat aatcgctatc actgcgattt
      241 acttttcttt gcatagattg actcagaaaa atgtttaagg gtgggtggca tgtttgttcc
      301 gtttctcatt atgttgcgcg aagggcttga agccgcgctg attgtcagtt tgattgccag
      361 ctatcttaag cgtacccagc gaggccgatg gattggtgtg atgtggattg gcgtgttgct
      421 tgccgctgcg ttgtgcctgg gcttgggtat cttcattaac gaaaccaccg gcgaatttcc
      481 gcaaaaagag caggaactgt ttgaaggtat cgtggcggtg atcgccgtgg tgatccttac
      541 ctggatggtt ttctggatgc gcaaagtgtc gcgcaacgtc aaagtgcaac tggaacaggc
      601 agtcgatagc gcattgcagc gtggaaatca tcatggctgg gcgctggtga tgatggtctt
      661 ttttgccgtt gcaagggaag gcctggagtc ggtctttttc ctgctggcgg catttcaaca
      721 agatgtcggg atctggccgc cgctgggtgc aatgctcggt cttgctactg ccgtggtgct
      781 cggcttcctg ctctactggg gcggtattcg cctcaatctt ggtgcatttt ttaaatggac
      841 cagcctgttt attctcttcg tcgccgcagg gctggcagct ggtgccattc gcgcatttca
      901 tgaagccgga ttgtggaacc actttcagga aatcgccttc gatatgagcg aggtcctctc
      961 aactcactcg ctgtttggca cgctgatgga agggattttt ggctatcagg aagcaccgag
     1021 cgtcagcgaa gtcgccgtct ggtttattta tctcatcccg gcgctggtgg catttgccct
     1081 gccaccacgt gcaggggcga cagcgtctcg ctctgcgtag caaatacgac gcaaactctt
     1141 gcttagttac aacatacttt aaagggatag tctcgtcatg accattaact tccgccgtaa
     1201 cgcattgcag ttgagcgtgg ctgcgctgtt ctcttctgct tttatggcta acgccgctga
     1261 tgtgccgcag gtcaaagtga ccgtgacgga taagcagtgc gaaccgatga ccattacggt
     1321 taacgccggg aaaacacagt tcattattca gaaccacagc cagaaggcgc tggagtggga
     1381 gatcctcaaa ggcgtgatgg tggtggaaga gcgggaaaat atcgcccctg gttttagcca
     1441 gaaaatgacg gcgaatttac agcctggcga atacgatatg acctgcggtc tgctgactaa
     1501 cccgaaaggg aagttgattg tcaaaggtga ggcaacggcg gatgcggcgc aaagtgatgc
     1561 gctgttaagt cttggtggtg caattactgc atataaagcg tatgtcatgg cggaaaccac
     1621 acagctggtg accgacacca aagcctttac cgacgcgatt aaagcaggcg atatcgaaaa
     1681 agcgaaagca ctgtatgcgc cgacgcgcca gcactatgag cgcattgaac cgattgctga
     1741 actgttctcc gatctggatg gcagcattga cgcccgtgaa gatgattacg agcaaaaagc
     1801 tgctgatcca aaattcaccg gtttccaccg tctggaaaaa gcattgtttg gcgacaacac
     1861 caccaaaggg atggataagt atgccgacca gctttatacc gatgtggtcg atttgcaaaa
     1921 acgcatcagt gaactggctt tcccaccttc aaaagtggtc ggcggcgcag ccggactgat
     1981 tgaggaagtg gcagccagca aaatcagcgg tgaagaagat cgctacagcc acaccgatct
     2041 gtgggatttc caggctaacg ttgaaggctc gcagaaaatt gtcgatctgc tgcgtccaca
     2101 actgcaaaaa gccaacccgg aactgttggc aaaagtcgat gccaacttta aaaaggtcga
     2161 taccattctg gcgaaatacc gtactaaaga cggttttgaa acctacgaca aattgaccga
     2221 tgccgaccgg aatgcactga aaggaccgat tactgcgctg gcggaagatc tggcgcaact
     2281 tcgcggtgtg ctgggactgg attaagcgtt atgcagtata aagatgaaaa cggcgtgaat
     2341 ggaccgtcac gccgacgttt actgaaaggg attggtgcac tggcgctggc gggaagttgt
     2401 ccggtcgctc atgcacaaaa aacgcaaagt gcgccgggta cgctttcacc ggatgcacgc
     2461 aatgagaaac agccgtttta tggtgagcat caggcaggga tcctgacgcc acaacaggcc
     2521 gcaatgatgc tggtggcgtt tgatgtgctt gccagcgata aagccgatct tgagcggttg
     2581 tttcgcttgt tgactcagcg ttttgctttt ctgactcagg gcggagcagc accagaaacg
     2641 ccaaatccgc gcctgccacc actcgattcc ggcattcttg gcggctacat tgcgcccgat
     2701 aatctcacca tcacgttatc ggtgggtcac tcattgtttg atgagcgctt tggccttgcg
     2761 ccacagatgc caaaaaagct gcagaagatg acgcgtttcc ccaacgactc gctggatgcg
     2821 gcgttatgtc atggtgatgt gttgttacag atttgcgcca acacccagga cacggtgatc
     2881 catgcgctgc gcgatatcat caaacacacg ccggatttgc tcagtgtgcg ctggaagcgg
     2941 gaagggttta tttccgatca cgcggcgcgt agtaaaggca aagagacgcc gattaatttg
     3001 ctgggtttca aagacggcac tgccaatccc gatagccaga atgataagtt gatgcaaaaa
     3061 gtggtgtggg taacggcaga tcagcaggag cttgcgtgga caatcggtgg cagctatcag
     3121 gcagtacgct tgattcagtt tcgagtggaa ttttgggaca gaacgccgct gaaagaacag
     3181 cagacgattt ttggccgtga taagcaaacc ggtgcgccgc tgggaatgca acatgagcat
     3241 gatgtgcctg attacgccag cgacccggaa gggaaggtga ttgcgttgga cagccatatc
     3301 cggctggcga atccccgcac ggcggagagt gagtccagcc tgatgctgcg tcgtggctac
     3361 agttattcac tgggcgtcac caactccgga caactggata tggggttgct gtttgtctgc
     3421 taccagcacg atctggaaaa aggcttcctg acagtacaaa aaaggctcaa tggcgaagcg
     3481 ctggaggaat acgttaaacc tatcggcggc ggttattttt ttgcgctgcc gggagtgaag
     3541 gacgcgaacg attatctcgg aagcgcatta ttgcgggttt aatgttttta ggcggataag
     3601 gcatttgtgc gcagatgcct gatgcgacgc ttgcgcgtct tatcatgcct acaatcagtg
     3661 cgggtttggt aggctggata aggcgttcac gccgcatccg gcgatcgtgc actgatgcct
     3721 gatgcaaatc ctgctgaaag cacacagctt ttttcatcac tgtcatcact ctgtcatctt
     3781 tccagtagaa actaatgtca ctgaaatggt gttttatagt taaatataag taaatatatt
     3841 gttgcaataa atgcgagatc tgttgtactt attaagtagc agcggaagtt cccggcagtg
     3901 atagtcagtc actatggaga tcgcggatgg taacgtcctg tactggacat gttttagata
     3961 atcaacgcgc caccactcgc ggagttttct cctccggtag tcatctcgtc actttgcatt
     4021 ttcaacctca tcctttcttt tcatgtgtta ccgacgccgt aaacggtgcg cgtagccgtt
     4081 tttcggcatt ttatccaaaa gcaaactatg gcttacaagg aagccaaccc tctgatgttc
     4141 gtgcgcataa tcgcgctgcc aacggcgcgt gtgatgaata caaacaactc aaggtgctct
     4201 ccatgggaag acaaaaagca gtgatcaaag ctcgtcgcga ggcaaaacgt gtgctgagac
     4261 gggattcgcg cagccataag cagcgtgaag aagaatcggt cacctcgctt gtgcagatga
     4321 gcggcgtaga agccattggt atggcccgcg acagtcgcga tacctcgccc atcctcgcgc
     4381 gaaatgaagc gcaattgcac tatctgaagg ctattgagag taagcagctg atattcgcca
     4441 cgggcgaagc cgggtgcgga aaaacctgga tcagcgcagc aaaagcggca gaggccctga
     4501 tacataagga tgtcgacagg attatcgtca cccgtccagt tctgcaagcc gatgaagatc
     4561 ttggcttctt acctggagat atcgcagaaa agtttgctcc ctattttcgc ccggtatacg
     4621 acgtgctggt ccggcgctta ggggcttcct ttatgcagta ctgcctgcga ccggaaatag
     4681 ggaaggtgga aattgcgccg ttcgcctata tgcgtggacg tacctttgaa aatgcagtcg
     4741 tcattcttga cgaggcgcag aatgtgactg ccgcgcaaat gaaaatgttt ttaacccgcc
     4801 tcggggagaa cgtgacggtt atcgtcaacg gtgatatcac gcaatgcgat ttacctcgcg
     4861 gcgtgcactc cggattaagt gacgcactgg aacgtttcga agaagatgaa atggtcggga
     4921 tcgtccgatt cggtaaagag gattgcgtac gttcggcact ttgccaacgt acgctacatg
     4981 catacagtta agtgtattat cggtgcagag cccgggcgaa ccgggctttg ttttatttca
     5041 acagattttc acttcaggtt agcgttcagg ggggcgactt tgagggtgtt gataagctga
     5101 actggagtct ggtttagata ctcttaccag gcatttttcg tcatagcatc agaccagtgt
     5161 aatactgcca gcgcggagag tttttcttcc agaagccaaa aaaatgagca tctgcctatc
     5221 ttttttcggc cattcaacct gtacaggaga tctggtgcca attttgctta tgaggtttaa
     5281 tcaacaatgg gccatacttt gactgactgt ttaacaccga atatattcct ctgatttatt
     5341 ctttagggat aatattataa atgtaaggtt aatcttaatt aattctttgg ctaatattgt
     5401 gtgttgttgt ttttttgtgg aaggcaagcc gcaatggcta aatagggatg gttgtaataa
     5461 tcaaaacatg atactaacta tatattgatc gttgcattgt tggcatgtaa cacgcttcag
     5521 cgagtaacat tggtaatgaa atataatcaa catggatagg tgaacattaa ttatgagctt
     5581 tatgtctttt gtaacttgtt tgacaattgc atttatcgcc ggttttgctg ttgccagaaa
     5641 attgacactc cgaaaagttg cctcatcggt aatcatcgct aaatcgcaac cagtcccttc
     5701 atctgcaact tcccctgccg ctaaggcgtc gacaaggagc tatataccgc agaaaaccta
     5761 tcgtgactct cgttggcaag agcgagatga gttatttcag ggaatggttg ccgaaggtat
     5821 tttattggaa agaaaagagt ggttaatcac gcgttgtgaa aaaatgtatt tcgataatct
     5881 ggaaaaatgg tttggacatt attgtcatat ccattgccag gtctctctgg ggcgcttgat
     5941 caactttcca gaacaacctg agtttagcga agaagaaaga aagcgtttct ttgcaattta
     6001 taatggcatg gcaatggatt atgttctggt ttcaaagaag aacaataaaa tagtctgtgt
     6061 tattgagctg gatgacccga gtcatgaaag ggcggatcgc attattcgtg accgttggtt
     6121 ggatacgatt atgactcttg cacgggtgcc ttttgtacga gcttctgtca ctaaattgga
     6181 ggaaatccct ccggtctggg agactcgtga caaaattcgc ccattagtcg cggcgacaaa
     6241 gagtgaaccg ttgcattaac taagtatccc ggtcggggat tctcatcccc ccggtgtgtg
     6301 caatatacga aaaaaaagcc cgtactttcg tacgagctct tctttaaata tggcggtgag
     6361 ggggggattc gaacccccga tacgttgccg tatacacact ttccaggcgt gctccttcag
     6421 ccactcggac acctcaccaa attgtcgttc ctgtcttgct ggaacgggcg ctaatttagg
     6481 gaaatcatga cctgaggtca acaaactttt ttaaaaaatc gcgcgtttat tcaaacttca
     6541 atcaatgtgt ggttttaata agcgaaatct gctttttttg ccaccgacca cggatttgtt
     6601 atgctggtgg cctttgtaga tcataacgat aagtgcgagt aaatttcgcg caacgctttt
     6661 caggagtcag tatggatatc atcttttatc acccaacgtt cgatacccaa tggtggattg
     6721 aggcactgcg caaagctatt cctcaggcaa gagtcagagc atggaaaagc ggagataatg
     6781 actctgctga ttatgcttta gtctggcatc ctcctgttga aatgctggca gggcgcgatc
     6841 ttaaagcggt gttcgcactc ggggccggtg ttgattctat tttgagcaag ctacaggcac
     6901 accctgaaat gctgaaccct tctgttccac tttttcgcct ggaagatacc ggtatgggcg
     6961 agcaaatgca ggaatatgct gtcagtcagg tgctgcattg gtttcgacgt tttgacgatt
     7021 atcgcatcca gcaaaatagt tcgcattggc aaccgctgcc tgaatatcat cgggaagatt
     7081 ttaccatcgg cattttgggc gcaggcgtac tgggcagtaa agttgctcag agtctgcaaa
     7141 cctggcgctt tccgctgcgt tgctggagtc gaacccgtaa atcgtggcct ggcgtgcaaa
     7201 gctttgccgg acgggaagaa ctgtctgcat ttctgagcca atgtcgggta ttgattaatt
     7261 tgttaccgaa tacccctgaa accgtcggca ttattaatca acaattactc gaaaaattac
     7321 cggatggcgc gtatctcctc aacctggcgc gtggtgttca tgttgtggaa gatgacctgc
     7381 tcgcggcgct ggatagcggc aaagttaaag gcgcaatgtt ggatgttttt aatcgtgagc
     7441 ccttaccgcc ggaaagtcca ctctggcaac atccacgcat gacgataaca ccacatgtcg
     7501 ccgcgattac ccgtcccgct gaagctgtgg agtacatttc tcgcactatt gcccagctcg
     7561 aaaaagggga gagggtctgc gggcaagtcg accgcgcacg cggctactaa taaagcatca
     7621 ggattcctgc tatccttggc gggaattgaa tacaggagag agttatgtat cccgtcgacc
     7681 ttcatatgca taccgttgcc agcacacatg catatagcac attgagtgat tacattgccc
     7741 aggccaaaca aaagggcatt aaactttttg cgatcaccga tcatggcccg gatatggaag
     7801 atgcgccgca tcactggcac ttcattaaca tgcgtatctg gccgcgagtg gttgatgggg
     7861 tagggatcct gcgcggcatc gaagctaaca ttaaaaatgt tgatggtgaa attgattgca
     7921 gcggtaaaat gtttgactcg ctggatctga ttattgccgg ttttcatgag ccggtttttg
     7981 caccacatga caaagcgacc aatacacaag cgatgatcgc cactatcgcc agcggcaatg
     8041 tgcatataat aagccatccc ggaaatccga aatatgaaat agatgttaaa gccgttgctg
     8101 aagcagccgc gaaacatcag gtggcgctgg aaatcaataa ttcctcattt ttacactcac
     8161 gtaagggcag tgaagacaac tgtcgtgcgg tagccgcagc ggtacgtgat gctggaggtt
     8221 gggttgcatt aggctcggat tctcacaccg cgtttaccct gggggaattt gaagagtgtc
     8281 ttaaaatcct cgacgcggta gattttccgc cagagcgcat tttgaatgtt tctccgcgcc
     8341 gcttactgaa cttccttgaa tctcgcggta tggcaccgat tgcggaattt gcagaccttt
     8401 aattactagt aatggaaata tattaatgaa cgagttttct atcctctgtc gtgtgctggg
     8461 ttcgctctat taccgccaac cgcaagatcc tttactggtg ccgctgttta ccctgattcg
     8521 tgaggggaaa ctggctgcga actggccact ggagcaggat gagttgctga cacgtttgca
     8581 gaaaagctgt gatatgacgc aagtctctgc cgattacaat gcgttgttta tcggcgatga
     8641 atgtgccgtg ccgccatatc gtagcgcatg ggttgaggac gcaacggaag cggaagtgcg
     8701 cgcttttctt tctgagcgag ggatgccatt agcagatacg ccagccgatc acatcggcac
     8761 attgctgctc gcggcttcct ggctggaaga tcagtcaacg gaagatgaga gcgaagcact
     8821 ggaaacactg ttcagtgagt atctggtacc ctggtgtggc gcgttccttg gcaaagtgga
     8881 ggcccatgca accacgcctt tctggcgcac catggcaccg ctaacccgtg atgccattag
     8941 tgcaatgtgg gacgagctgg aagaggattc tgaagagtaa atgtgatcta cgtcactcat
     9001 aactgcaact gataatttgt tgttgcataa aatgtgtgct cgatctcatt catggccgcg
     9061 ttttctgcta tcatgcgcgg catgaacata cttctctcta ttgcaatcac aacaggcatt
     9121 ctctccggta tctggggatg ggtggctgtt tccctgggct tactaagctg ggcgggcttc
     9181 ctgggctgta cggcctactt tgcttgcccg caaggtgggc tgaaagggct ggcgatctcc
     9241 gctgcaaccc tgcttagtgg cgtggtgtgg gcgatggtca ttatttacgg tagtgcactg
     9301 acgccacatc tggaaattct cggttatgtc ataaccggta ttgtcgcgtt tctgatgtgt
     9361 attcaggcca aacagctgct gctttcattt gttccgggga cgtttatagg cgcatgcgcg
     9421 acgtttgccg ggcagggtga ctggaaactg gtgttacctt cgctggcgct tgggctggta
     9481 tttggttacg caatgaaaaa cagcggcctg tggctggcgg cgcgtagtgc aaagacggct
     9541 caccgtgagc aggaaatcaa aaataaagcg tgaggggcac tcacgctttc gcttaaacag
     9601 taaatgccgg attattaatt ctggcatttt tatctgtcag gattccggtg gaaccgacat
     9661 atggcggtat ttcaccagaa tgtcattctg ccgttctgct ttgttctgca aatcccacag
     9721 accacggtca ataccatcat taatcaggaa aatgacccct gtttcgatag ccgacatcag
     9781 gcacagcata acgggttcgt tcgaggtgta acccacttcc ccttccagca agcgctggta
     9841 gtcaataaag cggaaaaccc cggcctgaac ttcataggaa agtatcgtct tactggtgtt
     9901 caccgaggag aggatctcac cggtactcac attgacgacg cgcaggttca cggcaatctg
     9961 atcgagctgg tattgcgtgt cggcaccgat cccaaaatat cttgccccaa ccccgcccga
    10021 tttgacgttg ctttcataac cgataatcga accttcaacc atgatatttg ccgccgttaa
    10081 agattgcagc gggattcggt tattaatggc aaccgtgccg ttttcttgtg cagcacgaat
    10141 aatcttgcgc tcgttaagca ggttttgtaa gccctggcgc tccagcggta taaaccagcg
    10201 agaatctttc aatgccgtga ccagcatcgc cgtggcgctt tgcggaacag cagtggagaa
    10261 gttacttgcc gggtagggtt taaattgccc ggtttcgtcc tgaatgttgt ataccgaaac
    10321 aaagatctta cccgtcggcg ctggcagatg ggtcaaatct ttgtagctct gagcacgagg
    10381 cattaatgtc ggtctggcgg cttctttagg cggggcggtt aagcatccgc tcagtaacat
    10441 gacggcaacc aaaagaaata agcgctgcat gattattttc cttatgaagc tggggcttaa
    10501 aaatcggttg agttattttg taaacccgaa acctggatgg tcgaggtttg tccggtttta
    10561 cgatctgtca cgttcaactg caattgacca tcgcggttag caatatcaac aatataatcg
    10621 ttggtcacca tgcggcccgg tttaccggta ttaatattcg acagtagccc acctaaaatt
    10681 tgtgactgga tggcctgagt aaagttatct aacgctgagg gtgtttcaat accaaagtca
    10741 tcgttatagc tcggatcttt ataagagttt tgggcctgag cgctatttaa taaaaaagcg
    10801 ccattatttg ggttaccacc aaagtttgga ttacggaact ggaaagtcat ggttccagcc
    10861 caacttaatg gcgaaataag catgagtaga actactgcat gtttgacacg cattgcagcc
    10921 tccgaacaat tttttattta gaattcatca tgcgccaaat cgcccgtact taataacgcc
    10981 tgatttatct ggcgacgatt tagtgcttct tcagtttgaa tcagtgcaaa gacgacagtt
    11041 ttttcgaagt ctcttttcaa cgggaataaa aaagtctgga aaataacgtc ctgattcacc
    11101 gttatagtga tccaacttcc ccatcgtgca ctgggccttt cattaatcgt taagttaccc
    11161 gtatagtcac tttcccattt atcactaaag gctcggtaaa aatcatggcc aatagatgaa
    11221 acagtatggt cagttaacaa tcccgggact tctacctcaa cggcgtgaag attccctgcg
    11281 gcgaacagaa attctgccgc cacaatccag cgtaaataac gtttcatggc tttatcgcct
    11341 gaggttatcg tttgcccagg aaaccgcttg tgtccggttt tttacggcta tcttcttgaa
    11401 aagattataa agatgcgttt taaccgtatt ttcgctgatg aacaacgaac gagcgatctc
    11461 gttattagac gcgccgatac gcagcttatt caggatctct ttttcccgat gagtaaggag
    11521 ggctgattcc gtgctgttat aacgatagtt accagaatgt gtaatcaggt aactggcaag
    11581 cttttgcgta aagtagcatt cgccgcgcag gacgccttgc aatccattga caacgcgttc
    11641 ttgatcctcc atggcataaa aaacgccgtt gatatgaggc cagttttcaa tgtcgcggta
    11701 cgggtaatct tcaggcgtat ttagcaacaa tattttgata ttgttgtttt tcctgctcaa
    11761 agtatcctgc caataatgga taagcttttt atccgcttcc atcatatcca gaagaataat
    11821 agagcctgaa gatatatcgt ctagagaacg ttgaatatta tgtaattttc ctgtaattgc
    11881 cagcgattgt ttaaggtgct gcaagagagc cgtcgcctgc aaagaaggtt tagtgatcaa
    11941 caataatgta tgaccatgaa tactatggac ttcattaaac atgatgaaac cccgcttttt
    12001 ttattgatcg cacacctgac agctgcctct aaaatagaag caccagaagt actgacagat
    12061 gttgcactgc tgtgtgtagt aataaatcaa ccctaaa
//
LOCUS       DAPGOF010000149        12086 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.150, whole genome shotgun sequence.
ACCESSION   DAPGOF010000149 DAPGOF010000000
VERSION     DAPGOF010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12086
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.150"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            266..1036
                     /locus_tag="RHV13_003630"
     CDS             266..1036
                     /locus_tag="RHV13_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HDV1243120.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1027..1500
                     /locus_tag="RHV13_003631"
     CDS             1027..1500
                     /locus_tag="RHV13_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HDV1243121.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1607..2146
                     /gene="dnaT"
                     /locus_tag="RHV13_003632"
     CDS             1607..2146
                     /gene="dnaT"
                     /locus_tag="RHV13_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HDV1243122.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2149..2886
                     /gene="dnaC"
                     /locus_tag="RHV13_003633"
     CDS             2149..2886
                     /gene="dnaC"
                     /locus_tag="RHV13_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HDV1243123.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2935..3429
                     /locus_tag="RHV13_003634"
     CDS             2935..3429
                     /locus_tag="RHV13_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HDV1243124.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSATSELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3683..5974
                     /gene="opgB"
                     /locus_tag="RHV13_003635"
     CDS             3683..5974
                     /gene="opgB"
                     /locus_tag="RHV13_003635"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HDV1243125.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFNNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTMWKGKTAFKDTVIDMARYKSNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6113..7135)
                     /gene="lgoD"
                     /locus_tag="RHV13_003636"
     CDS             complement(6113..7135)
                     /gene="lgoD"
                     /locus_tag="RHV13_003636"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HDV1243126.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7274..8188
                     /locus_tag="RHV13_003637"
     CDS             7274..8188
                     /locus_tag="RHV13_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDV1243127.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8403..9764
                     /locus_tag="RHV13_003638"
     CDS             8403..9764
                     /locus_tag="RHV13_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1243128.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHNFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLVVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9813..11477)
                     /gene="tsr"
                     /locus_tag="RHV13_003639"
     CDS             complement(9813..11477)
                     /gene="tsr"
                     /locus_tag="RHV13_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HDV1243129.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11596..12042)
                     /gene="hpaR"
                     /locus_tag="RHV13_003640"
     CDS             complement(11596..12042)
                     /gene="hpaR"
                     /locus_tag="RHV13_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HDV1243130.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
BASE COUNT         2937 a         3188 c         2942 g         3019 t
ORIGIN      
        1 ttgttaacat taaaaaacgt gcccggttat ctattatcct gattcgtaaa ggccaaacaa
       61 taaattcatt atagtgataa tattcatgtg atagtttgtt tgcggcgaga gataattcgc
      121 tttttatcac cgagcttgtc ccgataacca ggattaatca gcgcaatttt gatcttaacc
      181 taccgcaaaa caaccgtgct tcatgataca aatggattct cattatctga acaatacgca
      241 gcacgtctat gacaaagggc gagttatgca aactgagcaa cagcgagccg taacacggct
      301 ttgtatccag tgtggattat ttcttttgca acatggtgcg gaaagcgcgt tggttgatga
      361 gctttcctca cgactgggtc gggcgctggg aatggacagt gtcgaaagtt ctatctcttc
      421 gaacgccata gtgctgacaa ctattaaaga tgggcaatgc ctgacatcga cacgtaaaaa
      481 tcacgatcgc ggcattaata tgcatgtggt gactgaagtc cagcacattg tgattcttgc
      541 ggagcatcat ctgctggatt acaaaggcgt agagaaacga tttagccaaa ttcagccatt
      601 acgttaccca agatggctgg tagccttaat ggttggcctt tcttgcgcct gtttctgtaa
      661 actcaataac ggtggctggg atggtgccgt catcaccttc tttgccagta cgaccgcgat
      721 gtatatccgc cagctgctgg cacaacgtca tcttcatcca cagatcaact tttgccttac
      781 cgctttcgcc gccaccacca tttccggatt gcttttgcaa ctccccactt tcagcaatac
      841 ccccaccatt gcgatggccg ccagcgttct gctgctggtg ccgggctttc cgttgattaa
      901 tgccgtcgcc gatatgttta aaggccacat caataccgga ctggcacgct gggcgatcgc
      961 cagtctgctg acactggcta cctgcgtcgg cgtggtgatg gcactgacga tttgggggct
     1021 acgcggatgg gtgtgatcga atttctgttt gcattggcgc aggatatgat cctcgccgcc
     1081 attcctgcgg tcggctttgc gatggtgttc aacgttcccg tgcgggcgtt acgctggtgt
     1141 gcgctgcttg gctcgatagg tcatggttcc cgaatgatct tgatgaccag cgggttgaat
     1201 attgagtggt caacctttat ggcttctatg ctggtcggca ccattggtat tcaatggtcg
     1261 cgctggtatc tggcgcatcc gaaagtgttt accgtggcgg ccgttatccc tatgttcccg
     1321 ggcatatcgg cttataccgc aatgatttcg gcggtaaaaa tcagccagct aggttacagc
     1381 gaaccgttga tgattaccct gttaaccaac tttcttacag cttcatcgat tgttggtgcg
     1441 ttatccatcg gtctttccat tcctggatta tggttgtacc gcaagcgccc tcgcgtataa
     1501 aattgcctcg cttaagcaca cggatgagag acagcctcct ctcctccgtg tgttactata
     1561 aaagttatct cccttctcgt tcatcgttcc atatttgaga aacagtatgt cttccagagt
     1621 tttgaccccg gacgtcgttg gtattgacgc cctggtacac gatcaccaaa ccgttctggc
     1681 aaaagctgaa ggcggtgtgg ttgccgtatt tgctaacaat gccccggcgt tttatgccgt
     1741 cacgcctgca cgcctggctg aactgctggc gctggaagaa aagctggcgc gtccgggaag
     1801 cgatgtcgct ctggacgatc aactctatca ggaaccgcaa gccgctcccg ttgctgtacc
     1861 catggggaaa ttcgccatgt atccggactg gcaacccgat gccgacttta tccgcctggc
     1921 ggcgctatgg ggcgtggcgc taagagagcc ggtaaccacc gaagaactgg cctcattcat
     1981 tgcctactgg caggcggaag gtaaagtctt tcaccatgtg cagtggcaac aaaaactggc
     2041 gcgcagcctg caaatcggtc gtgccagcaa cggcggactg ccgaaacgag atgtgaatac
     2101 ggtcagtgaa cctgacagcc aaattccacc aggattcaga gggtaacgat gaaaaacgtt
     2161 ggcgacctga tgcaacgcct gcaaaaaatg atgcctgccc atatcaaacc cgcattcaaa
     2221 acgggtgaag aactactggc ctggcaaaaa gaacaagggg cgatccgctc cgccgctctc
     2281 gaacgtgaaa atcgggcgat gaaaatgcag cgcaccttta accgctccgg tattcgtcca
     2341 ctgcatcaga actgctcctt tgagaactat cgcgttgagt gtgaagggca gatgaatgcg
     2401 ttaagcaaag cgcgccagta tgtcgaagag tttgacggca acatcgccag ctttatcttt
     2461 tctggtaagc caggaaccgg caaaaaccat ctggcggcgg caatctgcaa cgagctgctg
     2521 ctacgcggta aatccgtatt gatcatcacc gtggccgata ttatgtcggc gatgaaagat
     2581 accttcagga atagcggtac cagcgaagaa caactgctta acgatctgag caacgttgat
     2641 ctgctggtga tcgatgagat cggcgtgcag accgaatcga aatacgaaaa agtgatcatc
     2701 aaccagatcg tcgatcgccg ctcttcttcc aaacgcccaa ccgggatgct gaccaacagt
     2761 aatatggaag agatgaccaa gttgctgggc gaacgcgtga tggaccgtat gcgcctgggt
     2821 aacagtttgt gggtgatctt caactgggat agctaccgta gccgggtaac aggtaaagag
     2881 tattaatccc cgtttctggc ggtatactgc gacacaattc caccttgagt aatgatgatg
     2941 aaaactgtta aacatcttct gtgctgtgcc attgccgcca gcgcattaat ttccaccggg
     3001 gtgcatgctg cgtcctggaa agatgcgctc tccagcgcca ccagcgaact tggcaaccaa
     3061 aacagcacga cacaggaagg cggttggtcg ctcgcgtcat taactaactt gcttagcagc
     3121 ggaaaccagg ccttaagcgc agataacatg aacaatgccg caggcattct gcaatactgc
     3181 gcgaagcaaa agctggcgtc ggtaaccgat gccgaaaaca tcaagaatca ggtgctggaa
     3241 aagctgggcc tgaacagtga agagcaaaaa gaagacacca actatctgga cggcattcag
     3301 ggtttgctga aaaccaaaga tggtcagcaa ctcaatctgg ataacatcgg aacgactccg
     3361 ctggcagaaa aggtgaaaac caaagcctgc gatctggtgt taaagcaggg gctgaacttc
     3421 atttcctgaa ccgcgaaacg ccgcgtttta gctgttaccc ggctgacgcg gctctctcct
     3481 gcccttttcc ctgctcctgg acggttttcc ccctgttaca cgggaagcga ccgcgatcaa
     3541 aaaagtctgt ttataaacta attcctaaca acatcgcatt tttttgacac tagaattgca
     3601 gcaatatggc gacgccatta cattgtgctt ctcaaaaagc ttaaacgttg gctaaacggc
     3661 ccggtttagt gaggatcgcc cgttgtcaga actactctct ttcgcccttt ttctcgcctc
     3721 tgtgctgatt tacgcatgga aagcgggacg taacacctgg tggtttgcag ccacattaac
     3781 ggtgctgggg ctatttgtcg ttttaaatat caccctgttt gccagcgact attttactgg
     3841 cgatggtatt aacgacgcgg ttctctatac cttaaccaac agcctgaccg gtgctggcgt
     3901 cagcaaatac atcctgccgg gtatcggcat tgtgctgggg ctgacagcgg tgttcggtgc
     3961 gctgggctgg atcctgcgcc gtcgtcgcca tcatccgcac cattttggtt acagcctgct
     4021 ggcgctctta ctggcgctgg gttcggtgga cgccagcccg gcatttcgtc agataacgga
     4081 actggtgaaa tcccagtcac gcgatggcga cccggacttt gcggcttatt ataaagagcc
     4141 gtcgaaaact atccctgacc cgaaactcaa cctggtttat atctacggcg aaagtctcga
     4201 gcggacctat tttaataacg aagctttccc ggatctcacg cctgaactgg gcgcgttgaa
     4261 aaatgaaggc ctggatttca gccacacgca gcagctgcca ggaacggatt acacgattgc
     4321 gggcatggtg gcttctcagt gcggcatacc gctgtttgcc ccctttgaag gcaacgcctc
     4381 cgcctctgtc tccagcttct tcccgcagaa catctgtctg ggcgatatcc tgaaaaactc
     4441 gggttatcag aactatttcg tgcagggcgc gaatctgcgt tttgccggta aagatgtgtt
     4501 cctgaagtcg catggcttcg accacttata cggctcagaa gagctgaaaa gcgtggtggc
     4561 cgacccgcac tatcgcaacg actggggatt ctacgacgat accgttctcg atgaagcgtg
     4621 gaaaaagttt gaagagcttt cccgctcagg tcagcgattc tcactgttta ccctgacagt
     4681 cgatacccat cacccggatg gttttatctc gcgtacctgt aaccgcaaaa aatatgattt
     4741 tgacggtaag ccgaaccagt cattcagcgc ggtaagttgc agccaggaga atatcgcggc
     4801 gtttatcaac aaaatcaaag cgtcaccgtg gtttaaagat accgtcatcg tcgtctcttc
     4861 tgaccattta gcgatgaaca acacggcgtg gaaatacctt aataagcagg atcgcaataa
     4921 cctgtttttt gtcattcgtg gcgacaagcc gcagcaagag acgctggcag tgaagcgtaa
     4981 cacgatggat aacggcgcga cggtgctgga cattctcggt ggcgataact atctcggact
     5041 tggtcgtagc agtttatccg ggcagtcgat gtcggaaatc ttcctcaata tcaaagagaa
     5101 aacattggcg tggaagccgg atatcatccg cctgtggaaa ttccctaaag agatgaaaga
     5161 gttcactatc gaccagcaga aaaacatgat tgccttctcg ggtagccatt tccgtttgcc
     5221 gctgctgttg cgggtttcag acaagcgcgt agaaccgctg ccggaaagtg aatactcagc
     5281 accgctgcgt ttccagctgg ccgatttcgc tccacgcgac aatttcgtct gggttgaccg
     5341 ttgctacaag atggcacaac tttgggctcc ggaactggca ctctccaccg actggtgtgt
     5401 ctcgcagggg cagcttggcg gtcagcaaat tgtccagcat gttgataaaa caatgtggaa
     5461 gggcaaaacg gcatttaaag atacggtcat cgacatggcg cgttacaaaa gcaatgtcga
     5521 tacgctgaaa attgttgata acgatattcg ctacaaagcc gacagtttca tcttcaacgt
     5581 cgccggtgcg ccagaagagg tgaaacagtt tagcgggatt tcccgtccgg agtcgtgggg
     5641 ccgctggtcc aacgctcagc tgggcgatga agtaaaaatc gagtacaagc atccgctgcc
     5701 gaagaaattt gacctggtga ttaccgccaa agcatacggc aataacgcca gccgtcctat
     5761 tccggtacgc gtaggcaatg aagaacaaac tcttgtgctg ggcaatgaag taaccaccac
     5821 cacactgcat ttcgataacc caaccgatgc cgacacactg gtaattgtgc cgccggaacc
     5881 tgtctcaacc aacgaaggga atatcctcgg acactcgccg cgtaagctcg ggatcggcat
     5941 ggtggaaatt aaagttgtgg aacgtgaagg gtaattcctg actgatgtgt aggtcgcata
     6001 aggtgcgacc tacacacctt ctccggccta caaaacatca ttgagaaaca gaaaaaaaca
     6061 gcgcccaaca cttccccgga cgctgcatgg caactttaat aatctcgcct tatcagaaag
     6121 taattacacc cttaatcaac tcacgattgt taatcacatc gcgctcgtag gtttctgcca
     6181 gcgtggcgaa cggatagcga tgggttaaca tcatgtcggc agtgattttc ccttctgcca
     6241 tcagtcgacc gactttagcg aaatcttccg gcgtggcgtt gcggctgccc atcatcgtcg
     6301 tttctttttt atggaattcc ggatcggaaa actgcaactc acctttaaac aggccgacaa
     6361 ataccaccgt gccgccgtga cgaatcagat tcacggtgtt attcatcgca tgttgattac
     6421 ctgtcgcgtc gatcactttc tgcgccagcg aaccaccaaa ctgcgcccgt agctgcgcgt
     6481 caaaatcttc ggctgacggg tccagtacag gtaattccag acgcgttgcc acatgttcac
     6541 ggcgcgctgg actggtatcc gccaccacca cctgtgcgcc atcggcttta gcgattgccg
     6601 ccgcgcccag accgattggc cccgccccga ccaccagcac ctgctcgccg ggagcaatgg
     6661 ctgcgcgacg caccgcatga gcgctaatag cgaaaggttc aatcaatgct gccgcctgcg
     6721 ggtcaatacc gtctgcgggc aaaatgttcg ccaccggcac gctcaaatac tcactaaaac
     6781 cgccatcctg atgcacgcca atgactgaaa ttttttcaca gcaattggta cgcccgcttt
     6841 tacacgccgg gcattgctga caggcaacat aagggatcac agcaacttgc tgaccatttt
     6901 taagatcagc aatattttta cccagcccaa caatctcccc acatatttca tggcctaaaa
     6961 cacgtggata actaaaaaat ggttgatttc caccccaggc atgaatatcg gtaccgcaaa
     7021 tcccgacaga cttaattttt attaatgctt cattgtcacc cggaataggt atctcgcgtt
     7081 gtttccagac taattctttc ggctgctggc aaattaaaac attcatcgta gacatgattc
     7141 ggctctccat tttttgttgc cttagatatc aatgaaaatg aaaaatggca gcggaacgga
     7201 aaatctgttt tgtgaaaaca cacgcataaa aatgttttaa atcgggtttt aatggactca
     7261 caaggaggaa aatatgagtc gttcacaaaa tttacgccac aatgtgatta accaggtcat
     7321 tgatgatatg gcgcggggtc atatcccttc cccacttcca tcgcaaagtg cgctggcgga
     7381 aatgtacaac atcagccgca caacagtgcg tcatattctc agccacttac gcgaatgcgg
     7441 cgtcttaacg caggttggaa acgactatgt tattgcgcgt aaacctgacc atgatgacgg
     7501 tttcgcctgt actaccgctt caatgagtga acaaaacaaa gtgtttgagc aggctttttt
     7561 cacgatgata aaccagcgtc agttacgccc tggggaaact ttttctgaac tgcaactggc
     7621 tcgggccgcg ggtgtcagcc cggtggttgt aagagaatat cttttaaaat tcggccgtta
     7681 taatcttatt caaagcgaaa aacgcggcca gtggagcatg aagcaattcg atcaatccta
     7741 cgccgagcag ctgtttgagc tacgcgaaat gctcgaaaca cattcacttc agcactttct
     7801 caacctgcct gatcatgatc cccgctggct gcaggcgaaa accatgctcg aacgtcatcg
     7861 gctactgcgt gacaacatcg gcaatagctt tcgcatgttc tcgcaacttg acagagattt
     7921 tcactcctta ctgctttcag ctgccgacaa tatctttttt gatcaatcgc ttgagatcat
     7981 ctcagtcatc tttcattttc actatcaatg ggacgaaagc gatcttaaac agcgcaatat
     8041 cattgccgtc gatgagcata tgaccattct cagcgctctg atttgccgca gtgaccttga
     8101 tgccaccctt gcgctccgta accatttgaa ttcagctaaa caatcaatga ttcgatccat
     8161 taatgagaac acgcgttatg ctcattaagt acggattaaa aacagcaaac tattagcggg
     8221 tgcacggttt tatcaaaaga taaataccgg gaaattcccg cttacctatg ctcacaatca
     8281 gtcgactaaa aatgtttttg tgagcggtag taaagtccta aaactttaac ctgccgttaa
     8341 tgtcaacctt gcgtaatgca caattacgta aacaattaaa attataaata caggagtccg
     8401 gcgtggaaaa agaaaatatc accctcgatc cgcgttcttc atttactcca tcttcgtcgg
     8461 cagatattcc cgtgccacca gatggattag ttcaacgcag tacccgaatt aaacgcattc
     8521 aaaccaccgc catgttgtta ttattttttg cggcggtaat caattatctc gaccgcagtt
     8581 cgctgtcggt agcaaattta acgattcgtg aagaattggg attaagtgcc accgaaatcg
     8641 gcgctttgct ctccgtgttt tcactcgctt acgggattgc gcaacttcct tgcggcccac
     8701 tattggatcg taaaggccca cgcctgatgc tgggactggg gatgttcttc tggtcactgt
     8761 tccaggcaat gtctggcatg gtgcacaact ttacgcagtt cgtgttggtg cgtatcggta
     8821 tggggattgg tgaagcgccg atgaacccat gcggtgtaaa agtcattaac gactggttca
     8881 acatcaaaga gcgcggacgc ccgatgggct tcttcaacgc agcttctacc attggcgttg
     8941 ccgtaagccc accgattctg gcggcgatga tgctggtgat gggctggcgc gggatgttta
     9001 ttaccattgg tgtactgggg atttttctcg ccatcggctg gtatatgctc tatcgcaacc
     9061 gcgagcacgt agaactgact gccgttgaac aagcttatct caatgcaggt agcgtcaatg
     9121 cccgccgaga tccgctcagt tttgccgaat ggcgcagcct gttccgtaac cgtacaatgt
     9181 ggggaatgat gctcggattc agtggcatca actacactgc gtggctgtat ctggcctggc
     9241 ttcctggtta cctgcaaaca gcctataacc tggatttaaa aagcacaggg ttgatggcgg
     9301 ctatcccttt cctgtttggg gctgccggga tgctggtcaa cggttacgtt actgactggc
     9361 tggtcaaagg gggaatggct ccgattaaaa gccgtaagat ctgcattatt gccgggatgt
     9421 tctgttctgc cgcctttacg ctggtcgtac cgcaagcgac aacatccatg acagcggttc
     9481 tgctgattgg catggcactg ttctgtattc actttgccgg aacatcctgc tggggcttga
     9541 tccacgtcgc agttgcttct cgcatgactg cgtcggtggg cagtatccag aactttgcca
     9601 gcttcatctg cgcctctttt gcgccgatca ttactggttt tattgttgat accacccact
     9661 cattccgtct ggcactaatc atctgcggtt gcgtcaccgc agcgggggca ctggcgtaca
     9721 tcttcctggt tcgtcagccg atcaacgacc cacgtaaaga ttaatctccc tatgcccgat
     9781 aacattttgc ttatcgggca tttttatggc gattaaaacg tttcccagtt ctccccgcta
     9841 tctgccactg ccattttacg cggcgtagct ggcgtcacgg tttttaccac agccgatgtt
     9901 tcacgctgct gttgctgctg ttgctgaatc cggaacactg ccacagcttc ggtcaggcga
     9961 ctggcctgct cttccagcgc ggcggcggcg gcggcagatt cttccaccag cgcggcgttc
    10021 tgttgagtta cccggtccat ctcagcaacc gctaagccaa cctgatcgat accacggctc
    10081 tgctcatcag aagcagaagc tatttcgccc ataatgtccg tcacgcgggt cacagcactg
    10141 acaatctccg ccattgtttc cccggcgctt tcgaccagcg tagagccaac atccactttc
    10201 cccaccgagt cttcaatcag gcttttaatt tcacgagccg cctgggcgct acgctgggcc
    10261 agattacgca cttctcccgc aaccaccgca aaaccgcgcc cttgctcacc cgcacgcgca
    10321 gcctcaaccg ccgcgttcaa agccagaata ttggtctgga aggcaatgcc gtcaattacg
    10381 ctgataatat cggcgatttt ctgcgaactg gtggagatat cgcgcatagt ctgcaccacg
    10441 ttatccacca ctttgccgcc gcgttgcgcc gtttcagaag cacttaacgc cagatggctg
    10501 gcctggcgcg cattttcggc gttctgcttc accgttgcgg tcagttgctc catgctggct
    10561 gccgtctctt ccagcgaagc ggcctgttgc tcggtgcgcg aagagagatc gttattgccg
    10621 gtggcgattt cgctggcacc gctatagatg gcattggccc cgttgcgcac atcaccgacg
    10681 gtacgcatca gctctccctg catatggcgc aaactctctg ccagttgccc catctcatta
    10741 gagccatcca cctcaatcgg tttcaccaga tcgccgcctg caatatgacg aatgctgtca
    10801 atcaggcgat tcattggcgc taccagcgag gctttaatac cgaaccagac ggcgaagatg
    10861 accgccagta cgacgatcat cacgcccacc agaatccaca tagcctggct gtaggaggca
    10921 ttgttatcgc tgacggcgat atcatagagc cgatcgtttt gctccatgta agccacatac
    10981 tgcttctcga aaccgtcctg atatccctgg gtcggctgat caaagaactc gttgattttg
    11041 cctgcaccta atagttggat cagctccgcc agcgcattgt gataaatatc gtaattacgt
    11101 ttgatctctg ccgctgcggc ggtgctctga cgcgggtcac gcggcaacgc ttcgtaatcc
    11161 gcccagtttt tttccgcctg tttcagcgaa atactggcac tctgcatcag ctcagcaacg
    11221 gttgaaccgc taccaatatt attctgatcc atcatgtagc ggatacccgc gcggttgagg
    11281 gtgttacgcg tctgcaacaa cgcgacccag ctgccattca gcgtggattg ctgctggcga
    11341 atggtttgta aaacagtgaa attttctttg tcattcttta aggcattaaa gaacagaccg
    11401 cctgatgtca gttgtaaaag gccaaaaaca gccaaaacca gcagtaagct ggtcacgatc
    11461 ttgatacgtt ttaacatggt ttctctttcc tgtggaccga tgcaagattt tcggcctgga
    11521 aagcaaaaac tttatgaaaa ggtgtaaccc gccatccatg gaaaaaccag gcgctggtta
    11581 tcagatacta aaaagttatt cattatcgcc cgggatggct tcttgtcggg aattgcccag
    11641 agcaataaat tcctctaaca gatgggttaa ctgttgcatt ttctcggcag taaactgagc
    11701 ttcaatctgc cgataggctt cttcaatctg tgtctgagcg cggttataca gcgcctgccc
    11761 ctctttggtt agcgagatat agagcttccg ctgatcattg atcggcttta atcgcaacac
    11821 taaaccgtcg cgttccatcc gcgtaaggat cccggtcagg cttgggcgca aaatgcaggc
    11881 gcgatacgcc agatcgtgaa aatccattga tgggctttcc gccagaatac gcacgatgcg
    11941 ccactgttgc tcagtcaaat tatgccgttt aacgattggg cgaaaataac tcatcgccgc
    12001 ttctcgcgcc tggagcaacg caatggttag agagtcgtgc attatctttc ccctgaaaaa
    12061 ttcaaaatca ttaatataga aacagt
//
LOCUS       DAPGOF010000150        11980 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.151, whole genome shotgun sequence.
ACCESSION   DAPGOF010000150 DAPGOF010000000
VERSION     DAPGOF010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11980
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.151"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..1354)
                     /gene="uidB"
                     /locus_tag="RHV13_003641"
     CDS             complement(<1..1354)
                     /gene="uidB"
                     /locus_tag="RHV13_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HDV1243131.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPPD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     L"
     gene            complement(1351..3162)
                     /gene="uidA"
                     /locus_tag="RHV13_003642"
     CDS             complement(1351..3162)
                     /gene="uidA"
                     /locus_tag="RHV13_003642"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HDV1243132.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELYVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(3551..4141)
                     /gene="uidR"
                     /locus_tag="RHV13_003643"
     CDS             complement(3551..4141)
                     /gene="uidR"
                     /locus_tag="RHV13_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HDV1243133.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(4370..5137)
                     /gene="hdhA"
                     /locus_tag="RHV13_003644"
     CDS             complement(4370..5137)
                     /gene="hdhA"
                     /locus_tag="RHV13_003644"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HDV1243134.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(5249..6277)
                     /gene="malI"
                     /locus_tag="RHV13_003645"
     CDS             complement(5249..6277)
                     /gene="malI"
                     /locus_tag="RHV13_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HDV1243135.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            6452..8044
                     /gene="malX"
                     /locus_tag="RHV13_003646"
     CDS             6452..8044
                     /gene="malX"
                     /locus_tag="RHV13_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HDV1243136.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            8054..9226
                     /gene="malY"
                     /locus_tag="RHV13_003647"
     CDS             8054..9226
                     /gene="malY"
                     /locus_tag="RHV13_003647"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HDV1243137.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETSEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRIYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            9330..10331
                     /gene="add"
                     /locus_tag="RHV13_003648"
     CDS             9330..10331
                     /gene="add"
                     /locus_tag="RHV13_003648"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDV1243138.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQEQEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEIAFLSAEEKRALREKVAAK"
     gene            complement(10367..11407)
                     /locus_tag="RHV13_003649"
     CDS             complement(10367..11407)
                     /locus_tag="RHV13_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDV1243139.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            11650..11775
                     /gene="blr"
                     /locus_tag="RHV13_003650"
     CDS             11650..11775
                     /gene="blr"
                     /locus_tag="RHV13_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HDV1243140.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSTTVQHK"
BASE COUNT         2908 a         3138 c         2926 g         3008 t
ORIGIN      
        1 ttaattgctg ctgcactttt ttacgattat caatttcaac cacgatttct ttgaattttt
       61 tatccgtgag cggataaaac cagataataa cgaatgccag tagcataaat ccgcaaggta
      121 ctaaggcaat tgatgtgcgg attcccataa taacttccgg cgtttgcacc tgattggcga
      181 tatatccgct taatcccaaa ataaaggcag gaattgaacc tccgattgcc tgaccgcatt
      241 tacgggtaaa tgagaatagt gaataggtga gcccttcaat tcgcacgccg gtcaggtatt
      301 caccgtattc tacggtatca gcttccagcg cccacatcac ggtcatggta acgccctgac
      361 caattgaggc aatggctaac gcaaccagtg ccaccggcag cgaccagacg gaaacccaga
      421 agaacagcag ataaccgcag gttcccagca aagcgccaat caggaaggta ttctttttac
      481 cgatcctcgc gaccatcccc ggcaccaacg gtgccgatgc cacagtacca accaggtttt
      541 gcaccagtac cagcacagtg aacagcccgg tatcatttaa tacatagcgc acgtagaaca
      601 acgacgaggc gctgaccgcg aaggtcgaga tcagcacaca cagcgcaccg atgcacaaca
      661 taaacagcgg acgattccgt ttcagggttt gcagactgat cttcaatgac ggctgcgcca
      721 cgatacgtac cacattctca cgcgtcgatt tgaagcagat gaagtaaagc accattccgg
      781 ctatcgccag cacaattgtc cagaaatgat ataccgacac catctcttcc gggctggagt
      841 tcttaatgct cggccctatc agaaatgcca ggcagacaaa ggtcaatgaa gcggcaatcc
      901 cacgagccgc gcccagacgg gcgcgggatt gtggttgttg ggtcatcgcg gtagcaagtg
      961 aaccataagg aatattcacc aggctgtagc aaagcccgag gcccatgtag gtcaaatatg
     1021 catacactac tttgctgcta tggctccagt cgggcggcac ccagaatacc agcacgctga
     1081 agatcattaa cggcgcagta ccgaagagta aaaacgggcg gaattttccc cagcgggtat
     1141 tcacactgtc caccactcgt ccggcaaaga cgtcggcgaa ggcatcgaat acccgcacca
     1201 gtaacagcat ggtgcccgcc gcagcggcac cgacgccagc gacgtcggtg tagtaactca
     1261 acaggaagag cgcccccatt gcgaaggcga agttattggc gacgtcaccg aggctgtagc
     1321 cgacgatggt gcgccaggag agttgttgat tcattgtttg cctccctgct gcggtttttc
     1381 accgaagttc atgccagtcc agcgtttttg cagcagaaaa gccgccgact tcggtttgcg
     1441 gtcgcgggtg aagatcccct tcttgttacc gccaacgcgc aatatgcctt gcgaggtcgc
     1501 aaaatcggcg aaattccata cctgttcacc gacgacggcg ctgacgcgat caaagacgcg
     1561 gtgatacata tccagccatg cacactgata ctcttcactc cacatgtcgg tgtacattga
     1621 gtgcagcccg gctaacgtat ccacaccgta ttcggtgatg ataatcggct gatgcagttt
     1681 ctcctgccag gccagaagtt ctttttccag taccttctct gccgtttcca aatcgccgct
     1741 ttggacatac catccgtaat aacggttcag gcacagcaca tcaaagagat cgctgatggt
     1801 atcggtgtga gcgtcgcaga acattacatt gacgcaggtg atcggacgcg tcgggtcgag
     1861 tttacgcgtt gcttccgcca gtggcgcgaa atattcccgt gcaccttgcg gacgggtatc
     1921 cggttcgttg gcaatactcc acatcaccac gcttgggtgg tttttgtcac gcgctatcag
     1981 ctctttaatc gcctgtaagt gcgcctgctg agtttccccg ttgactgcct cttcgctgta
     2041 cagttctttc ggcttgttgc ccgcttcgaa accaatgcct aaagagaggt taaagccgac
     2101 agctgcagtt tcatcaatca ccacgatgcc atgttcatct gcccagtcga gcatctcttc
     2161 ggcataaggg taatgcgagg tacggtagga gttggcccca atccagtcca ttaatgcgtg
     2221 atcgtgcacc atcagcacgt tatcgaatcc tttgccgcgc aaatccgcat cttcatgacg
     2281 gccaaagcca gtaaagtaga acggtttgtg gttgatcagg aactgttcgc ccttcactgc
     2341 cactgaccgg atgccgacgc gcagcgggta gatatcacac tctgtctggc ttttggctgt
     2401 gacgtacagt tcatagagat aaccttcacc cggttgccag aggtgcggat tcaccacttg
     2461 caaagtcccg ctggtgcctt gtccagttgc aaccacctgt tgatccgcat cacgcagttc
     2521 aacgctgaca tcaccattgg ccaccacctg ccagtcaaca gacgcgtggt tacagtcttg
     2581 cgcgacatgc gtcaccacgg tgatatcgtc cacccaggtg ttcggcgtgg tgtagagcat
     2641 tacgctgcga tggatcccgg cgtagttaaa gaaatcatgg aagtaagact gctttttctt
     2701 gccgttttcg tcggtaatca ccattcccgg cgggatagtc tgccagttca gttcgttgtt
     2761 cacacaaacg gtgatacgta cacttttccc ggcaataaca tacggcgtga catcggcttc
     2821 aaatggcgta tagccgccct gatgctccat cacttcctga ttattgaccc acactttgcc
     2881 gtaatgagtg accgcatcga aacgcagcac gatacgctgg cctgcccaac ctttcggtat
     2941 aaagacttcg cgctgatacc agacgttgcc cacataatta cgaatatctg catcggcgaa
     3001 ctgatcgtta aaactgcctg gcacagcaat tgcccggctt tcttgtaacg cgctttccca
     3061 ccaacgctga tcaattccac agttttcgcg atccagactg aatgcccaca ggccgtcgag
     3121 ttttttgatt tcacgggttg gggtttctac gggacgtaac ataagggact cctcattaag
     3181 ataaaaatac tggtcaacct ttaatctgat tagttaacca attctgtgtt gcacacagtt
     3241 atctgtgaga aacccatgga agagaaatgg aaataaaaag aactgacacc gcgatcaaaa
     3301 aaccaaaaac tgcaataccc acagcgtgat attgatcgca ttaatcttta aaacggcgtt
     3361 gacagaagcc ataaaataag caaccgaacg ttcgttcgag catattggga acgataaact
     3421 ctcatgacat atggctacgg tgaatatttt ggggggggaa ttctccgatc ccggctagat
     3481 tgtattttgt acagggaaat taaagtatga aacgcgggca cagttatcac cagcccgcga
     3541 aatgagagga tcaggatgcg gttaagatac cgccaatcat cgcgcgtaat ccctgagcaa
     3601 acgacgcttc acgcgccaga tcttccgctt cgatatcggc cagtacaccg taggtcagat
     3661 ccagcagtaa acgtgaagtc attgacgtgt tgatgtctgg gcttatctcg cctttttgct
     3721 gggcatcacg catccgctgg gcaacaaatt cagtgatcgt catatgctta tttttaagca
     3781 tggcggcgac ctgtgggtta cgcatccctt ccgccataat ttcgaccacc agcgcccgtt
     3841 gcccaaaggc ttcatgggtg agagagacaa tagactcgac catatagtca acaaaatgaa
     3901 tcccttctat cggttcccgg aaacgggcca gcgccctctc ctggtcctgt aagataatcg
     3961 cctgaatcaa ggcttctttg gagatgaaat ggtgatagag cgtcccggga ctaatggcgc
     4021 aggatttaca gatggctttc atcgaggcac tgtgaaatcc attttctgaa aaaatctctc
     4081 tggcagcatt gaggatccgg gtccgtgtcg gttgtgcttc agtctgcatg ttatccatca
     4141 ttaaaccatt atagttagcg tgaataacta gtttaccgcg ttgtcaggtg agcgcctatc
     4201 attgctgttg aatagtgcga aggcacactc tattcgttta cttttcatcc agtaattggt
     4261 taaccaatag ccgtgatttg gctgccagtt gtttagcaga gacgttgatg aattgcacac
     4321 gctatcccgg taaacaacac atactgctgt ttaccggttc gttagtgtat taattgagct
     4381 cctgtacccc accaccggag acggtgagaa tttgtccgct tacccagctg gctgcaggcg
     4441 agcaaaggaa cagagctgcg ttagcaatat cttgcggttg gcccagacgt ctgatcggcg
     4501 tgtgctgtaa cattttttgt tcaatttctg gtgtaataac ggatttcagg gcatcggtta
     4561 atattgcccc cggcgcaatg ccatttaccc gaatattttt ttcacccagg tcaaacgcca
     4621 tatttctgac cagatgactg gccgcagctt tagatgatgc ataggaagtc atgtttatat
     4681 ttttattttc tgccgccata gaagtgatgg tcagaataac gccaccgcca tttttttcca
     4741 tttctggcgc aacaagttgt gacagatgga aaaaagaaaa cacattcagt tcataagcac
     4801 ggcgaaaatc cgccattggc atatcaaacg gtttaggtcc accgccaccg gcgttgttaa
     4861 ccagaatatc aactttaccc agcttactga tagcaaagtc tgccagtgca gagagttcct
     4921 gttcggaagt aatatcacaa cggcaggcaa atgcctgacc acccagttgt tgaatttcgt
     4981 ctacaacatg gttagctgcg tcggcgttaa tatcactgac caccacagat gcgccagctg
     5041 tcgcgaatgt aatggcgatt tctttaccaa tacctgcacc cgcacctgtg atgatggcgc
     5101 attttccgtc gagtctcagg ttgtcagaat taaacacata aacctcctgt gaacttcagt
     5161 aaaaataaaa ggcaccgcct gtgtgccaca ttttttatca ttaaccttga ggaaaggaca
     5221 ctaccaggta tagcagataa agagaggatt atttcgccgc aatgagccgg gcgggaataa
     5281 taagattgcg tgaatgcgtc tcttcatggg tgattttttg catcatgcga tccgcaagtg
     5341 tggtaccaag ttcccgcgca ggcgtgctgg cccaggtaac gggtatatca tcaagtgtgg
     5401 ttggtgtcgc atcggtaaat gccgccagcg aaacttgttg ctcaaagtaa cgatcgactc
     5461 cgctttcccc gctttgccgc cctgctttca gcaaaccaaa ccatgccccc atcgcaatag
     5521 tttcgttata gcaaaccacg gcactgatgg tcgggttatg acgtaaaagc gccgtgatag
     5581 cttccgcggc ttgcttctgg ctggaagtgc actccaacac ccaatcgctg tgaaacggca
     5641 ggccaaattt tagtagagtt gcacaatagc cccccacccg ttctgcacgg gttaatgagg
     5701 aactttgccc tcccagccag gcgatccgct gatgcccatt gcgaatgaga tgctccgtca
     5761 acaactgtgc agcctgcatg ttgtccgggc gaaccgtatc aacatcatca agataactgg
     5821 cacgggaagc gaaaatcacc gggatagctt tttcttctgc catccgtcgc aggtcatcgc
     5881 tgcttcctgc agccccggca attaccacac catcgacacc ctgattcagt aacagtgaaa
     5941 accgctgtgc cagctgctcg ccgtctttac cgccgtgaag caaaaaaacc atccgtccct
     6001 gcgcttccag agcttccgtc aatccggccg tcaattcggc gtaaaacggc gcagataaat
     6061 cacggacgat caaaccaatg acgccgcttt gcccgccgcg cagcgccgac gcctggcgat
     6121 tgcgcacaaa tcccagctct tcaatggcgg cgttaacgcg ttctcctgtg gcggtagaga
     6181 ttcgcccttt gccactaagc accagcgaaa cggtacttac cgacacgccc gcagccagcg
     6241 caacatcatg aatggttatt tttttggcgg tagccatgca gatgacctac tccctgatta
     6301 tgtgacagat aaaacgtttt accttttatt tatcttatac ccgctattat cgttgcgtaa
     6361 tgtgatttat gcctcactaa aatttgataa aacgttttat cttctcgcgc aatttactga
     6421 atccagattg ttctctacga ggagtcgttt tatgacggcg aaaacagcac cgaaagtcac
     6481 gctgtgggag ttcttccagc agttaggcaa aaccttcatg ttacccgtgg cattattgtc
     6541 gttctgcggc attatgctcg gcattggtag ttctcttagt agccatgatg tcataaccct
     6601 gatcccggtc ctgggcaacc ccgtgttgca ggctatcttt acctggatga gtaagattgg
     6661 ctcgtttgct tttagtttcc tgccagtcat gttctgtatc gccatcccgc tgggtctggc
     6721 acgcgaaaac aaaggcgtag cggcattcgc tggcttcgtc ggttatgcgg taatgaacct
     6781 cgcggtaaac ttctggttga ccaataaagg cattctgcca accacggacg ccgcggttct
     6841 gaaagccaat aacatccaga gcattcttgg gatccagtcg atcgacaccg ggatcctcgg
     6901 tgcggtgatc gccggtatta tcgtctggat gctgcatgag cgattccata atatccgcct
     6961 gccggatgcg cttgcattct tcggcggtac gcgcttcgta ccaatcatct cctcgctggt
     7021 gatgggcctt gtcggcctgg tgattccatt agtctggccg attttcgcga tgggtattag
     7081 cggcttgggc catatgataa acagcgcggg tgatttcgga ccgatgctgt ttggtaccgg
     7141 tgaacgtctg ctgttgccgt ttggtctgca tcacattctg gtggcattaa ttcgctttac
     7201 cgacgcaggc ggcacgcagg aagtctgcgg tcaaaccgtc agcggcgcat tgaccatctt
     7261 ccaggcgcaa ttgagttgcc cgaccactca cggtttttct gaaagcgcca cgcgtttcct
     7321 ttcgcaaggc aaaatgcctg cgtttctcgg cggtctgcca ggtgcagcgt tagcgatgta
     7381 tcactgcgcg cgcccggaaa atcgccataa aattaaaggg ctgctgattt ctggcctgat
     7441 cgcctgtgtc gttggcggca cgaccgaacc gctggaattc ctgttcctgt tcgtggcgcc
     7501 agttctgtat gtcatccacg cgctgttaac cggcctcggc ttcaccgtca tgtctgtgct
     7561 cggcgtcacc atcggtaata ccgacggcaa tatcatcgac ttcgtggtgt tcggtatttt
     7621 gcatggtctg tcaaccaagt ggtacatggt gccagtggtg gcggcaatct ggtttgtcgt
     7681 ttactacgtc atcttccgtt tcgctatcac ccgcttcaat ctgaaaaccc cggggcgcga
     7741 tagcgaagtt gccagctcaa tcgaaaaagc cgttgccggt gcgccgggta aatcaggtta
     7801 caacgttcct gcaatcctcg aagccttagg cggtgccgac aatattgtca gcctcgataa
     7861 ctgcattacc cgtctgcgtt tgtctgtgaa agatatgtcg cttgttaatg tgcaggcact
     7921 gaaggacaat cgggcaattg gcgtagtaca acttaatcaa cataacctgc aggttgttat
     7981 cgggccacaa gttcagtcag taaaagatga aatggccggt ctgatgcata ctgtccaggc
     8041 ataaggataa gatatgttcg atttttcaaa ggtcgtggat cgtcatggca catggtgtac
     8101 acagtgggat tatgtcgctg accgtttcgg cactgctgac ctgttaccgt tcacgatttc
     8161 agacatggat tttgccactg ccccctgcat tatcgaggcg ctgaatcagc gcctgatgca
     8221 cggcgtattt ggctacagcc gctggaaaaa cgatgaattt ctcgcggcta ttgcccactg
     8281 gttttccacc cagcattaca ccgcaattga tacgcagacg gtggtgtatg gcccttctgt
     8341 catctatatg gtttcagaac tgattcgtca gtggtctgaa acaagtgaag gcgtggtgat
     8401 ccacacgccc gcctatgacg cattttacaa ggccattgaa ggtaaccagc gcacagtaat
     8461 gcccgttgct ttagagaagc aggctgacgg ctggttttgc gatatgggca agctggaagc
     8521 cgtgttggcg aaaccagaat gtaaaattat gctcctgtgt agcccacaga atcctaccgg
     8581 aaaagtgtgg acgtgcgatg agctggagat catggctgac ctgtgcgagc gtcatggtgt
     8641 gagggttatt tccgatgaaa tccatatgga tatggtttgg ggcgagcagc cgcatattcc
     8701 ctggagtaat gtggcgcggg gagactgggc gttgctaacg tcgggatcga aaagtttcaa
     8761 tattcccgcc ctgaccggtg cttacgggat tatagaaaat agcagtagcc gcgatgccta
     8821 tttatcggca ctgaaaggcc gtgatgggct ttcttcccct tcggtactgg cgttaactgc
     8881 ccatatcgcc gcctatcagc aaggcgcgcc gtggctggat gccttacgca tctatctgaa
     8941 agataatctg acgtatatcg cagataaaat gaacgccgcg tttcctgaac tcaactggca
     9001 gatcccgcaa tccacttatc tggcctggct tgatctacgt ccgttgaata ttgacgacaa
     9061 cgcgttgcaa aaagcgctta tcgagcaaga gaaggtcgcg atcatgccgg ggtataccta
     9121 cggtgaagaa ggtcgtggtt ttgtccgact caatgccggc tgcccacgtt cgaaactgga
     9181 aaaaggtgtg gctggattaa ttaacgccat ccgcgctgtt cgttaacccc aattgcgcaa
     9241 cgtaaaaaat cgttgcgcaa tcgtggattt ttaccctgct ttgtttttat aatggtgcgc
     9301 acttttatat ccagaaaaag agtgcgacca tgattgatac caccctgcca ttaactgata
     9361 tccatcgcca ccttgatggc aacattcgtc cccagaccat tcttgaactt ggccgccagt
     9421 ataatatctc gcttcctgca caatccctgg aaacactgat tccccacgtt caggtcattg
     9481 ccaacgaacc cgatctggtg agctttctga ccaaacttga ctggggtgtt aaagttctcg
     9541 cctctcttga tgcctgccgc cgcgtagcat ttgaaaacat tgaagatgca gcccgtaacg
     9601 gcctgcacta tgtcgagctg cgtttctcac caggctacat ggcaatggca cataagctgc
     9661 ctgtagcggg tgttgtcgaa gcggtgatcg atggtgtacg tgaaggttgc cgcacctttg
     9721 gtgtgcaggc gaagcttatc ggcattatga gccgaacctt cggcgaagcc gcctgtcagc
     9781 aagagcagga ggccttttta gcccaccgtg accagattac cgcacttgat ttagccggtg
     9841 atgaacttgg tttcccggga agtctgttcc tttctcactt caaccgcgcg cgtgatgcgg
     9901 gctggcatat taccgtccat gcaggcgaag ctgccgggcc ggaaagcatc tggcaggcga
     9961 ttcgtgaact gggtgcggag cgtattggac atggcgtaaa agccattgaa gatcgggcgc
    10021 tgatggattt tctcgccgag caacaaattg gtattgaatc ctgtctgacc tccaatattc
    10081 agaccagcac cgtagcagag ctggctgcac atccgctgaa aacgtttctt gagcatggca
    10141 ttcgtgccag cattaacact gacgatcccg gcgtacaggg agtggatatc attcacgaat
    10201 ataccgttgc cgcgccagct gctgggttat cccgcgagca aatccgccag gcacagatta
    10261 atggtctgga aattgctttc ctcagcgctg aggaaaaacg cgcactgcga gaaaaagtcg
    10321 cagcgaagta acaaaaatgg atggtgcaaa ttgcaccatc catttttcat gcaaggcaca
    10381 aagtcgcgcg atgtttggcg gattcgatgc ccagctcaat caactccatt acctggattg
    10441 cctggcttgc cggaaccgga ttttcaccat cgccatttaa cgcatcacga atagccgcat
    10501 agtaagccgg ataattccca ggcaccgtca acagcgtttc ttcgacacgt tcctcacctt
    10561 ccacgcgggt aagtacgcca tcacgcatat cgtagcccca gtcttcctgc ggcagacgct
    10621 cgccattttt cagacgttct tcctgtggat cgaggccata tttcacataa ctgcctcggg
    10681 atccatgcac gatataccgt gctgactcag cggctgccag catggtaccg tgtaaaatga
    10741 ctcgccgctg tggataggac aagatggcgt ggaaataatc ggtcgactgc gctccaggcc
    10801 gtaactgtgc caagtccacc gtcatgctga ccggtaaacc aaataatgta atagcctgat
    10861 caagaagatg cggtgctaaa tcgtaccaga taccactgcc tgggccaccc tgttcacgcc
    10921 aacgatcgcg cacctgcgga cggaagcggt caaaatgaga ctcaaagtaa gcgacttcac
    10981 ccagcacgcc ttccgcgagt aaacctttta gcgtcaagaa atcgctatcc cagcgacggt
    11041 tatggaatac agacagcaca cgccccaggc tttttgccag cgcatccagc tctcgcgctt
    11101 gtgacagtgt cacggtaaag ggtttatcaa cgaccacatg tttacccgcc tcaagcgctg
    11161 ctttggctaa cgggaaatgg gtatcgttgg gtgtaggaat gacaatcagg tctatgttgg
    11221 gatcgttaaa cagatgcttc ggctcagaga caaccgtaac cgttggccag tcggctttta
    11281 cttttgtttc atcactgctg gagattaccg ccagttccag cccgggcgtg cccgcaatca
    11341 ggggcgcatg gaaggttttg ctcgcataac catacccaat caacccaaca cggatgttgt
    11401 cgctcatgtc attgcctctc attgaagtat gatggctatt tgacactatc ctttaccctc
    11461 gctcaacagt ttaataacct gccagcaata agggatgttg tttaacttaa gtcaaaaaaa
    11521 tagcgatttt tccaacgaca aaagctaaat atcgcaaaaa cctcagtaaa aatcttgctg
    11581 gagctattat tgctaagtaa catttacccc ctgaagttaa tggatcaatc aacagagatg
    11641 tgagttgtaa tgaatcgtct tattgaatta acaggttgga tcgttcttgt cgtttcagtc
    11701 attcttcttg gcgtggcgag tcacattgac aactatcagc cacctgaaca gagtactacg
    11761 gtacaacaca agtaagctct gcgcttgtag agcgacatgc tgcccgtccg ggtgcatgtt
    11821 ttcacttgtc ggatattaaa ccaggaattt attatcttgt tcggtgttgt tggtgattgt
    11881 cagggatagt aaagcgcgtt actcttttca tagcagtttt tcatccctga atatttgcca
    11941 tcagcgcatt cttattattg taataacaca agcgttcact
//
LOCUS       DAPGOF010000151        11910 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.152, whole genome shotgun sequence.
ACCESSION   DAPGOF010000151 DAPGOF010000000
VERSION     DAPGOF010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11910
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.152"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            13..615
                     /locus_tag="RHV13_003651"
     CDS             13..615
                     /locus_tag="RHV13_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HDV1243141.1"
                     /translation="MNINYPAEYEIGDIAFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQIPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(617..1138)
                     /gene="ruvC"
                     /locus_tag="RHV13_003652"
     CDS             complement(617..1138)
                     /gene="ruvC"
                     /locus_tag="RHV13_003652"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HDV1243142.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(1173..1913)
                     /locus_tag="RHV13_003653"
     CDS             complement(1173..1913)
                     /locus_tag="RHV13_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDV1243143.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(1942..2394)
                     /gene="nudB"
                     /locus_tag="RHV13_003654"
     CDS             complement(1942..2394)
                     /gene="nudB"
                     /locus_tag="RHV13_003654"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HDV1243144.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(2512..4284)
                     /gene="aspS"
                     /locus_tag="RHV13_003655"
     CDS             complement(2512..4284)
                     /gene="aspS"
                     /locus_tag="RHV13_003655"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDV1243145.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            4594..5160
                     /locus_tag="RHV13_003656"
     CDS             4594..5160
                     /locus_tag="RHV13_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDV1243146.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILNAL"
     gene            5157..5975
                     /locus_tag="RHV13_003657"
     CDS             5157..5975
                     /locus_tag="RHV13_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDV1243147.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKMLPEIGAVPAIPQQSSLF"
     gene            6028..6423
                     /locus_tag="RHV13_003658"
     CDS             6028..6423
                     /locus_tag="RHV13_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HDV1243148.1"
                     /translation="MVSALYAVLGALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            6464..7207
                     /gene="cmoA"
                     /locus_tag="RHV13_003659"
     CDS             6464..7207
                     /gene="cmoA"
                     /locus_tag="RHV13_003659"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HDV1243149.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HNAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            7204..8175
                     /gene="cmoB"
                     /locus_tag="RHV13_003660"
     CDS             7204..8175
                     /gene="cmoB"
                     /locus_tag="RHV13_003660"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HDV1243150.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(8340..10769)
                     /gene="torZ"
                     /locus_tag="RHV13_003661"
     CDS             complement(8340..10769)
                     /gene="torZ"
                     /locus_tag="RHV13_003661"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HDV1243151.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYSQFEEYLTGKSDNTPKSAVWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFAELAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAVQA"
     gene            complement(10794..11894)
                     /locus_tag="RHV13_003662"
     CDS             complement(10794..11894)
                     /locus_tag="RHV13_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HDV1243152.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         2705 a         3169 c         2915 g         3121 t
ORIGIN      
        1 tggaatgaca acgtgaatat taattaccct gctgaatatg aaattggaga tatcgccttt
       61 acatgtatag gtgctgcctt atttggtcaa atatcagctg catcaaattg ttggagtaat
      121 cacgtcggga tcattatcgg tcataacggt gaagacttcc tggttgcaga aagccgggtc
      181 cccctttcaa ccatcaccac gctatctcgt tttattaaac gttcttctaa tcaacgctat
      241 gctataaagc gattagacgc tgggctgaca gaacaacaaa aacaacgaat tgttgaacag
      301 attccctccc ggctacgcaa actctaccac accggtttta aatacgaatc ttcgcgccag
      361 ttctgttcaa aatttgtctt tgatatttat aaagaggcgc tatgtattcc ggtgggtgaa
      421 atagaaacgt ttggagaact gttaaatagc aatccgaatg caaaactcac tttctggaaa
      481 ttctggttcc tgggttctat tccgtgggag cgtaaaaccg tcacgccagc cagtttgtgg
      541 catcatccgg gtttggtgtt gattcatgcg gagggagtgg aaacgcctca gccggaactg
      601 accgaggcgg tataacttaa cgcagtcgcc ctctcgccag gttcagccgc gattcgctca
      661 tctgcatcgc attctgactg acgtgacaat gggtaatcgc gatagctaac gcatcggcgg
      721 catccgcctg tggattagcg ggcagtttca gcaaggtgcg gaccatatgc tgcacctggc
      781 ttttttcggc actaccaata cctaccaccg tttgctttac ctgacgtgcc gcgtattcaa
      841 ataccggcaa ttcctgattc accgccgcaa caatcgccac gccgcgcgcc tggcccagtt
      901 tcagggctga gtcagcgttc ttcgccataa agacttgttc aatggcgaaa taatcaggct
      961 ggaactgggt gatgatttcc gtcacgcccg catagatgag cttcagacga gacggtaaat
     1021 catccacttt ggtgcgtatg catccgctac ccaggtagga cagttgcctg cctacctggc
     1081 gaattacgcc atagccggtc acgcgcgaac ccggatcaat gccgagaata atagccatca
     1141 cgcgtctccg ttttgctgtt tagcaggcct catcagagag tcgctgcgac ctcatcagag
     1201 atttcaccgt tatggtaaac ttcctgcacg tcgtcgcaat cttccagcat atcgatcaga
     1261 cgcatcagtt tcggtgcggt ttctgcatcc atatcagctt tggtggacgg gatcatggaa
     1321 acttccgcgc tgtctgcttt cagacctgcc gcttccagag cgtcgcgcac tttacccatt
     1381 tcttcccatg cagtgtagac atcaatcgcg ccgtcatcat aggtcacaac gtcttcagca
     1441 cctgcttcca gagctgcttc catgatggtg tcttcatcgc ctttctcgaa ggagatcacg
     1501 ccttttttgc tgaacagata ggctacggaa ccatctgtac cgaggttacc gccacatttg
     1561 ctgaatgcat gacgcacttc agcaacggta cggttgcggt tgtcagacag acattcaatc
     1621 atgattgccg tgccgccagg accgtaacct tcgtagatga tggtttccat gtttgcatca
     1681 tcatcaccgc ccacaccacg tgcaattgcg cggttcagtg tgtcacgggt catgttgtta
     1741 gacagtgctt tatccaccgc cgcacgcaga cgcgggttag cgtccggatc gccaccgccc
     1801 agcttagccg cggttaccag ctcacgaatg attttagtga agattttacc gcgcttagca
     1861 tcctgcgcag ctttacgatg tctggtgttg gcccatttac tatgacctgc cataaaaata
     1921 tctccagata gccctgcctg ttcaggcagc gttaattaca aactgttcaa tcgcctgccg
     1981 gttgctccag gacttagtga gcgccgccgc agcaggcgca tcaagccact tgtaagccag
     2041 atgttcagtg aaaacgatct gccgctcgtg cggaagcgca agacagaacc atgattccgt
     2101 attacgcgtc acgcccggcg catagcgatg acgtaaatgt gaaaaaattt caaactctac
     2161 cgtgcgctga cagtcaatta aggtcagttg ttcagcgaca acatcaatgg tgacctcttc
     2221 ctttacttcg cgcatggcag cttgcggcgc ggtttcaccc tcttccacgc tgccggttac
     2281 cgactgccag aaatcgggat cgtcacgccg ctgcaacatc agcacccgtt tcgtatcttg
     2341 tgcatagatg accactaaga tcgaaacggg acgcttatac actttatcct tcacgctgcc
     2401 tcttcgttga ctgccttcgc tcatcccatt cacatagtta tctatgctca tgggagttca
     2461 ctcagttgcc gccttgatgc aacgcgaatg atttcgtgta tttgagtcat atcagttatt
     2521 ctcagccttc ttcacaacct gaatgctcag ctcagccagt gcagtcgggt tagcaaagct
     2581 cggtgcttca gtcatcagac acgctgccgc cgtggttttc gggaaggcga taacgtcacg
     2641 gatattgtcg gtgccggtca gcagcatggt cagacggtca agaccgaatg ccagacctgc
     2701 gtgcggcgga gtaccgtatt tcagagcgtc gagaaggaag ccgaatttct cgcgctgttc
     2761 ctcttcgttg atacccagaa taccaaacac cgtctgctgc atatcaccat tatggatacg
     2821 tactgaacca ccgcccactt cgtaaccatt gatgaccata tcgtaagcgt tcgccaccgc
     2881 attttccggt gcagctttca gctctgcagc cgtcatgtct ttcggtgagg tgaacggatg
     2941 gtgcattgcc gtcaggccgc cttcaccgtc gtcttcaaac atcgggaagt cgataaccca
     3001 cagcggtgcc catttgcttt cgtcggtcag accaaggtct ttacccactt tcaggcgcag
     3061 tgcgcccatc gcgtcggcaa caattttctt gttgtcggca ccgaagaaaa tcatatcgcc
     3121 atcttgcgcg gcagtacgat ccaggatggc ttcgatgatt tctgcattaa ggaacttcgc
     3181 taccgggctg ttgatacctt ccagaccttt cgcgcgttcg ttaactttga tgtaagccaa
     3241 acctttcgcg ccgtagattt taacgaagtt accgtattcg tcgatctgct tacgggtcag
     3301 cgatgcgcca cccggaacac gcagagcggc aacgcgacct ttcggatcgt tcgccggacc
     3361 tgcaaatact gcaaactcaa cagatttcag cagatcagca acgtcggtca gttccatcgg
     3421 gttacgcaga tccggtttat cagaaccata acggcgttcg gcttccgcaa aggtcattac
     3481 cgggaaatcg cccagatcca cgcccttcac ttccagccac agatggcgca ccagcgcttc
     3541 catcacttca cgcacttgcg gcgcggtcat gaaagaagtt tccacatcga tctgagtaaa
     3601 ttcaggctga cgatcagcgc gcaggtcttc gtcacggaag catttaacga tctgatagta
     3661 gcggtcaaag ccggacatca tcagcagctg tttgaacaac tgtggggatt gcggcagtgc
     3721 gtagaattta cctttgtgca cacgagaagg caccaggtag tcacgcgcgc cttccggcgt
     3781 ggctttggtc agcatcggag tttcgatgtc gaggaagccg tggtcatcca taaaacggcg
     3841 caccaggctg gtgattttag cgcgggtttt caggcgctga gccatttccg gacgacgcag
     3901 gtcgaggtag cggtatttca gacgcgcttc ttcggtgttg acgtggttag agtcaagcgg
     3961 cagaacatct gcgcggttga tgatagtcag cgaggacgcc agcacttcga tttcgccggt
     4021 cgccatatcg cggttaatat ttttttcgtc acgcgcacgt acggtgcccg tgacctgaat
     4081 gcagaactca ttacgcagtt cagaggccag ctttaacgcg tccgcacgat ccggatcgaa
     4141 aaatacctgc acgatacctt cgcggtcgcg catatcgatg aagatcaggc taccaagatc
     4201 acgacgacgg ttgacccaac cacacagagt cacctgctgc cccacgtggg acaaacggag
     4261 ctgtccacaa tattctgtac gcatgagata tcccttaact tagctgccgg cggatgcccc
     4321 ctgcttcgca ggtgaccaag tcgcagcgtt agctgtatgt cacaactgaa tgaaaaaagg
     4381 cggctattat actggaaatt ctgccgcacc gtaagagcct ggcccgcgct ggaacgcctc
     4441 gttaccactt tatatcgggc ctgaaatcag actctacgcc agtttgctat aaaggtgttg
     4501 cccgaactca taaaaattaa caaaatttgt cgttccgcca tcggctaatc gcattaaggt
     4561 gtgaggcacg attttgtttt ttcaggagtc atcatgcttg aacttaatgc taaaaccact
     4621 gcgctggtgg tgatcgattt acaagaaggc atcttacctt ttgccggtgg tccacatact
     4681 gccgatgagg tagttaatcg cgccgggaag ctggcggcga aatttcgcgc cagcggtcag
     4741 cccgtgtttc tggtgcgcgt tggctggtct gccgattacg ccgaagcatt aaaacagccg
     4801 gttgatgccc cttcccccgc taaagtgttg cccgaaaact ggtggcaaca tccagctgca
     4861 ttaggtgcaa ctgacagtga tatcgaaata atcaaacgtc agtggggtgc gttttacggt
     4921 acggatctgg agttgcaatt acgccgccga ggtatcgata caatagtgtt atgtgggatc
     4981 tcgaccaata tcggagttga atccaccgcc cgcaatgcct gggaacttgg ctttaatctg
     5041 gtgattgccg aagatgcctg tagtgccgct agcgccgagc agcacaataa cagtatcaac
     5101 catatctacc cgcgcatcgc ccgtgtgcgt agcgtggaag agatcctcaa cgcgttatga
     5161 tttacatcgg tctaccgcaa tggtcgcatc ctaaatgggt gcggttgggg atcaccagcc
     5221 ttgaagagta tgcccgccac tttaactgcg tggagggcaa caccacgctt tacgccctgc
     5281 cgaaacccga ggttgtcctg cgctggcgtg aacagaccac agatgacttc cgcttctgtt
     5341 ttaagtttcc ggcgaccatt tcgcatcagg cagcattacg acattgcgat gatttagtga
     5401 ctgaattttt gacccgcatg tcaccgttgg ctccgcgcat tgggcaatac tggctgcaac
     5461 tgcctgccac attcggccca cgggagctgc ctgcgctttg gcattttctc gattctctcc
     5521 ctggcgaatt taattatggt gtggaagtcc gccatccaca gtttttcgcc aaaggggaag
     5581 aggaacaaac gcttaatcgc ggtttacatc agcgcggcgt taatcgggtg attttagaca
     5641 gccgcccggt tcatgcagca cgtccacaca gtgaagctat tcgcgacgct caacgaaaaa
     5701 aacctaaagt tccggtacat gctgtactga cggcgacaaa tccgctgatc cgttttatcg
     5761 gtagtgatga tatgacgcaa aaccgggaat tatttcaggt ctggttacaa aaattagcgc
     5821 agtggcatca gaccactacg ccttatcttt ttttacatac gccagatatt gcccaggccc
     5881 cggaactggt acataccctg tgggaagact tacgtaaaat gcttccagag atcggagcag
     5941 ttccggctat tccacagcaa tcttctcttt tctgaatttg ccacctatca tagacaggtg
     6001 ccatcggcca ttttaaaggg agtttgtatg gtaagcgcgc tgtatgccgt tttaggtgcg
     6061 ttgttattaa tgaagttctc ttttgatgtc gttcgcctgc gaatgcagta ccgcgttgcc
     6121 tatggcgacg gcggttttag cgaactgcaa agcgctattc gcattcatgg taacgcggtg
     6181 gaatatattc ccatcgcgat tgtgctgatg ctgtttatgg aaatgaatgg cgcagaaacc
     6241 tggatggtgc atatttgcgg catcgttttg cttgctggtc gtctgatgca ttattacggt
     6301 tttcatcacc gtctgtttcg ctggcgacgt tccggcatga gcgccacctg gtgtgcgctg
     6361 ttgctgatgg tgctggcgaa tctttggtat atgccctggg agttggtttt ctccctgcgt
     6421 tagcgcacaa tacgccactt tctttttccc ggatttttac gttatgtctc accgcgacac
     6481 gctattttct gcccctatcg ccagactggg tgactggacc tttgatgaac gggtagctga
     6541 agtcttcccg gatatgatcc agcgttccgt tcccggctat tccaatatta tttccatgat
     6601 tggtatgtta gccgagcgct tcgttcaacc tggtacgcag gtttacgatc tgggttgttc
     6661 tctgggcgcg gcgacgctct cggtgcgtcg caacattcat catgataatt gcaaaattat
     6721 tgccatcgac aactccccgg cgatgattga acgctgccgt cgtcatattg acgcctataa
     6781 agcccctacg ccagtagacg tcattgaagg tgatattcgc gatatcgcca ttgaaaacgc
     6841 atcgatggtg gtgctgaatt ttaccctgca attcctggaa ccttccgagc gccaggcgtt
     6901 actggataaa atttatcaag ggctgaaccc cggcggcgcg ctggtgcttt cggaaaaatt
     6961 cagtttcgaa gatgccaaag ttggtgaact gttgttcaac atgcaccacg actttaaacg
     7021 tgccaacggt tacagcgaac tggagatcag ccagaagcgc agcatgctgg aaaacgtgat
     7081 gctgaccgat tccgtggaaa cacataaagc acgcctgcat aacgccggtt ttgagcatag
     7141 tgagctgtgg ttccagtgct ttaactttgg ttcactggtg gcattaaaag cagaggacgc
     7201 tgcatgatcg actttggtaa cttttattct ctgattgcca aaaatcatct ttcacactgg
     7261 ctcgaaacgc tgcccgcgca gattgctaac tggcagcgcg agcagcagca cgggttgttt
     7321 aagcagtggt caaatgcggt ggagtttcta cctgaaatta aaccgtatcg tcttgattta
     7381 ttgcatagcg ttacggcaga aagcgaagag ccactgagcg cagggcaaat taaacgcatt
     7441 gaaacgctga tgcgcaacct gatgccgtgg cgcaaagggc cgttctcact gtatggcgtc
     7501 aacatcgata ccgaatggcg ttccgactgg aaatgggatc gtgttctgcc ccatctttct
     7561 gatttaaccg gacgcaccat tctggatgtc ggctgcggca gcggttatca catgtggcgc
     7621 atgattggcg caggcgcgca cctcgcggtg gggatcgacc ctacgcaact gtttctgtgc
     7681 cagtttgaag cggtgcgtaa actactgggt aatgatcagc gcgcgcattt gttaccgtta
     7741 ggtattgaac aacttccggc actgaaagcc tttgataccg tcttttcgat gggcgtgctt
     7801 tatcatcgtc gttcaccgct ggagcatctc tggcagttga aagatcaact ggtgaatgag
     7861 ggtgaactgg tgcttgaaac gcttgttatt gatggcgatg agaacacggt gctggtgcca
     7921 ggcgatcgtt acgcgcaaat gcgtaatgtc tatttcatcc cttccgcgct ggcgctgaaa
     7981 aactggctga agaagtgtgg ttttgttgat attcgcattg cggatgtgag cgttaccact
     8041 acagaagagc agcgacgcac tgaatggatg gttaccgagt ctctgtccga tttcctcgac
     8101 ccacatgatc cgagtaagac agttgaaggt tatccggcac ctaaacgcgc ggtgctgatt
     8161 gcgcgcaagc cgtaaaggtc tggtattact gcaggatgcg gcgtgaacgc cttatccggc
     8221 ctacaaaacc ttgctaattc aatatattgc agggactatg taggcctgat aagcatagcg
     8281 catcaggcag ctttacgttt gcataacctc agcgcccgtt tccgggcgct tttcacgtct
     8341 tatgcctgaa cagctggctg atcaaacgcc gttaacttcg gtgcgttacc cgtatatttt
     8401 tcgatataca ccagcgcaga gttaccggca caggcatttg ccagctgcga gctggggata
     8461 tccgccgtta acacgttcgc actgccgttt ttacacaagc cattttccag atctggccat
     8521 gcaccttcat gcacgcatac cacgcctttt ttgatcccgt cagtcaccac agcgcctgtc
     8581 aggatctgac cacgtttatt ccagacacgg accagatcgc cattcgcaat accaaagcga
     8641 gcagcatctt cggtgtgaat agtgattggt tcacgatcgg caaccgcata ttttttacgc
     8701 agttccgcat agttaagctg actgtgtaaa cggtgtgccg gatgtgcggt cagaagctgc
     8761 aactgcttct cgtcggcggt acccttccac tcatcaggcg caagccaggt tgggtgtgcc
     8821 gggcaatcct tatagccaaa tttttccagc gttttggagt aaatctcaat tttgccgctt
     8881 ggcgtaccca gcgcattttt caccggatcg gcgcggaaat caccataacg aacgtactgt
     8941 tcgttcttct cgctgcggcg catttcgatc agtttatttt gctgccagaa ggcattaaac
     9001 attggcatag tgacgcgttg cgcacgggca cctttctgag cggcatcata gaaaaatttc
     9061 agccacgcca tttcatcttt accttcggta tagatctctt ttccgccagg tttcagtaat
     9121 tccgcaagtt cggcaaacac gtcaaaatcg tttcgcgctt caaattgcgg agcgacagcc
     9181 tgcttcatcg gtacaatatg ctggttgctg taatcaccgg tcatcgtcaa gtcattgcgc
     9241 tcaaacgatg tggtgatcgg taatacgata tctgcatgtt tagcagctgc ggtccagtag
     9301 cattcagaaa cgacgatcat ctccggtttc tgccatgctt tgatcagacg attagtatcc
     9361 tggtgatggg taaagttacc accacccgcc caccagatca ttttgatatt cggatacgtt
     9421 tgttctttac cgttatgctg atatttgccg ccaggatttt ccagcgcatc gacgatacgg
     9481 gcaacaggaa tcgccgtcat tccgccatca tcagcggcct cgctggcctg tccggcaatc
     9541 gccgcagaca tttccggcag cacgccgcca acacgagtcg ggttgccacc gttggagtaa
     9601 tgataagaga aaccaaaacc gccgcccggt gtaccaatct gccctaacat cgccgccagc
     9661 gtcaccagca tccagtgttt ctgttcacca tattgctggc gctgaattcc ccagcctgcc
     9721 atcagcattg tgcgattcgc cgccatcagt tcagcgagtt tgactatctg cgcttccggt
     9781 acgccagtaa tttccgccgc ccagaccgcg ctcttcggcg tattatcgct cttacctgtc
     9841 agatactctt caaactgcga atacccggta gtgtattttt caaggaacac tttatcgtgt
     9901 ttgccttgtg tcatcagggt atgcgcaatc cctaacatca gtgccacgtc ggtgcccata
     9961 ttcggcgcga tccaggtggc attatcgcca aagaattcga tggtttcgga gcggatagga
    10021 tcaatggcaa tcactggttt gccagatttt ttcagctgat ggaagtattc cagcccttgc
    10081 tcatcggtac tgctccaggc aatttttaag gtattcagcg ggttcattcc ccacagcacc
    10141 acaacctggc tgttttccag aatcagcggc caggaggtct gctgttcata cacctctaca
    10201 gaaccgacca catgcggcat gatcacctgt gctgccccgg ttgaataatc gccgctatgc
    10261 ccggaataac cgcccgccag gttcatataa cgttgcagta aggtttgcgc tttatgcaac
    10321 acgccagaag agcgccagcc gtaagatccg gcaaaaatgg ccgatggtcc gttagcttta
    10381 cgaatacgct catgttgctc atgaatcagt tttaatgcct gttcccagct cacctgtaca
    10441 taggtatctt cgccacgacc tttcgccggt tgcagtggat tgtcgagata gctttttctc
    10501 accatcggat gctgaatacg cgcggtggtg tgtacctgat ccgccgccgt agactgtaag
    10561 gagttcggta tggttttcgc cagcgcgcct gtcgaagaaa caatcttgcc gtccttcact
    10621 tctacgttca tcgctcccca acgtcccgcg gtgaggattt taccgccctt ctcttctgcc
    10681 catgcgggta gcggtgctgc cgatgtcacc accagcgctc cagcggcaat accgctgtgt
    10741 ttaataaatt cacgtcttgt taatgtcata acttcctccc tgatcaacga ggatcactgt
    10801 ttctcggtaa tatctttggc gttgtactgg aaataccgcg ttaaaatgtc cagttcgttt
    10861 tcgctcatgc tggttcgtgc ccccattcct ttggcaatgg acggccacgc attgacggtg
    10921 taatggtcgg cggcaatagg ggcatgacaa ccagcgcaat aggtatcgtc aagtttttca
    10981 gcgtattgcc atagcggttt acggtccgct aatgcgggat cggtaagcgc accctgtaaa
    11041 gacgcctgac gccattgatt gccgtattcg tcagcctgcc attccccgtt taccgtgagc
    11101 gccttgatac cttcttcact taatgtggct agcgccagcc gttgacctgc cgccaggtag
    11161 agcgtgtttt cactgccctg catttgataa ccctgcaaca gaacgatcgg ctgtttgcca
    11221 ctggcatcaa cgacggtgag atcggtacca ggattcacgg tagccagctc gcctatgcga
    11281 gaagttttga aaggataaat atgtgcgcca ttagtcactg aagtagcggc ctgactttcc
    11341 agctcatgcg ccgcgttgtc atccattttt atttctggcg gaaaatgggc aatgccttta
    11401 tgacaatcga tacaggtttc gccgcccttt tgtgctttgt tatgcatttt ctgcgcagat
    11461 tcactttgcg aggcaatatc catggcatca aaagaatggc aactacggca cgttgcagag
    11521 tcagttgctt ttaattcttt ccatactgtt tcggccattt cctggcgatg agtttcgaac
    11581 ttatcgtcac tgtctatttt gccgctaaca aattcatgat aaatatcttt agatgctttt
    11641 aatttagcaa ataaataatc catccctgac tttggaatat ggcaatcggc acattccgca
    11701 cgtatccctt tctggttcga aaagtggaca gttccctgat attcctcaaa aggtttactc
    11761 atcgagtggc aggaaaggca aaatgctgta tccgacgttt tatgtaaggc tttttgcgcc
    11821 agtaataacc cgccgccacc aaccacaacc gctatcagca aaaacaataa cccaatgcgt
    11881 tttttccctc gcatagattc ctctaattaa
//
LOCUS       DAPGOF010000152        11883 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.153, whole genome shotgun sequence.
ACCESSION   DAPGOF010000152 DAPGOF010000000
VERSION     DAPGOF010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.153"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            65..880
                     /locus_tag="RHV13_003663"
     CDS             65..880
                     /locus_tag="RHV13_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(3')-I family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HDV1243153.1"
                     /translation="MSHIQRETSCSRPRLNSNLDADLYGYRWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTAFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAAFLRRLHSIPVCNCPFNSDRAFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            complement(1076..1840)
                     /locus_tag="RHV13_003664"
     CDS             complement(1076..1840)
                     /locus_tag="RHV13_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HDV1243154.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     gene            complement(1958..2026)
                     /locus_tag="RHV13_003665"
                     /pseudo
     CDS             complement(1958..2026)
                     /locus_tag="RHV13_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229646.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(2347..2847)
                     /locus_tag="RHV13_003666"
     CDS             complement(2347..2847)
                     /locus_tag="RHV13_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV1243155.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLVASCSDGIVGCCTLSAEDPEFWPDALKGEAAYLHKLAVRRTHA
                     GRGVSSALIEACRHAARTQGCAKLRLDCHPNLRGLYERLGFTHVDTFNPGWDPTFIAE
                     RLELEI"
     gene            complement(2974..3813)
                     /gene="sul1"
                     /locus_tag="RHV13_003667"
     CDS             complement(2974..3813)
                     /gene="sul1"
                     /locus_tag="RHV13_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HDV1243156.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPEDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            complement(3807..4154)
                     /locus_tag="RHV13_003668"
     CDS             complement(3807..4154)
                     /locus_tag="RHV13_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HDV1243157.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            complement(4372..5607)
                     /locus_tag="RHV13_003669"
     CDS             complement(4372..5607)
                     /locus_tag="RHV13_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEc58 family transposase"
                     /protein_id="HDV1243158.1"
                     /translation="MPRKKKTPVVTPAIDLPKEFLEKLIPGPMDAAGVEAVFQQLKKA
                     VIERALGAELGLHLADAEGGSGNHRNGRSGKTVLTDEGPLRIDVPRDRTGTFEPQLIP
                     KHERRFAGFDDRIVSMYARGMTVREIQGHLAEMYSVEVSPEFISKVTDEVMAEVTAWQ
                     ARPLEAMYPVVFFDALRVKIREDGVVRNKAVYLALGVLADGTRDILGLWIENTEGAKF
                     WMKVFNDLKTRGVNDILIGVADGLKGFPEALEAVFPATTLQTCIVHLIRNSLDYAGWK
                     DRKALAAAIKPIYTAPTAEAAEAELDAFEAGDWGERFPTVVAAWRRAWTRVVPFFAFP
                     PDVRRVIYTTNAIESVHARLRKIIKTRGHFPSDEAATKLIWLALRNITAEWSRASQHW
                     KAAMNQFAIIYEDRFTLAR"
     gene            complement(5875..7404)
                     /locus_tag="RHV13_003670"
     CDS             complement(5875..7404)
                     /locus_tag="RHV13_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV1243159.1"
                     /translation="MPRLTAPRRQAEVCAPLPAAHAAARYARHLPERTLLYALVQAHY
                     PDFIARLEAEDRPLPEYVREEFETYLRCGVLEHGFLRVVCEQCRAERLVAFSCKKRGF
                     CPSCGARRMAESARHLVEEVFGPRPVRQWVLSFPYPLRFLFASKPEAIGPVLGIVHRV
                     IAGWLADQAGVDHASAQCGAVTLIQRFGSALNLNVHFHMLWLDGVYEDTTERPQRKPR
                     LHRTRAPTSAQLTELAGTIAHRVCRHLSRRGWLEGEDESVFLSDSAGSDDGMDGLRMS
                     SMTYRIATGRDAGRKVVTLQILPGDAGSLEGDAGKVGGFSLHAGVAAEAHESHKLEKL
                     CRYITRPAISEQRLSISPQGRVRYQLKTPWRNGTTHVEWDAVDFIAKLAALVPPPRAH
                     LTRFHGVFAPNANLRAQLTPSGRGRRPAGDAVPVDASAHDEPRSPEQKRRAMSWAQRL
                     KRVFSIDVTTCAHCGGAVRIVASIEDPKAIRAILAHFEKHGALEQAHYRPAARAPPPA
                     A"
     gene            complement(7593..9230)
                     /gene="groL"
                     /locus_tag="RHV13_003671"
     CDS             complement(7593..9230)
                     /gene="groL"
                     /locus_tag="RHV13_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005411226.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDV1243160.1"
                     /translation="MAAKDIRFGEDARARMVRGVNVLANAVKATLGPKGRNVVLEKSF
                     GAPTITKDGVSVAKEIELADKFENMGAQMVKEVASKTSDNAGDGTTTATVLAQALIRE
                     GMKAVAAGMNPMDLKRGIDKAVTSAVEELKKISKPCSTSKEIAQVGSISANSDTDIGE
                     LIAKAMDKVGKEGVITVEEGSGLENELDVVEGMQFDRGYLSPYFINNPQSMQAELEDP
                     FILLHDKKISNVRDLLPILEGVAKAGKPLLIVAEDVEGEALATLVVNTIRGIVKVCAV
                     KAPGFGDRRKAMLEDMAILTGGTVISEEVGLSLEKATINDLGRAKKVQVSKENTTIID
                     GAGDTADIEARIKQIKAQIEETTSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGIVPGGGVALIRAKAAIAELKGANEDQNHGIAIALRAMEAP
                     LREIVTNAGDEPSVVLNRVAEGTGAFGYNAANGEFGDMIEFGILDPTKVTRSALQNAA
                     SIAGLMITTEAMVAEAPKKEEPAAPGGGMGGMGGMDF"
     gene            complement(9286..9606)
                     /gene="groES"
                     /locus_tag="RHV13_003672"
     CDS             complement(9286..9606)
                     /gene="groES"
                     /locus_tag="RHV13_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDV1243161.1"
                     /translation="MGSPTRKICAAINIKPLYDRVVIKRTEEEKVSAGGIVIPDSATE
                     KPIKGQVVAVGEGKVFDNGNVRAPKVKVGDQVLFGKYAGTEVKLDGTEYLVVKEDDIF
                     AVLG"
     gene            complement(9813..10712)
                     /gene="aac(3)-VIa"
                     /locus_tag="RHV13_003673"
     CDS             complement(9813..10712)
                     /gene="aac(3)-VIa"
                     /locus_tag="RHV13_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(3)-VIa"
                     /protein_id="HDV1243162.1"
                     /translation="MTDPRKNGDLHEPATAPATPWSKSELVRQLRDLGVRSGDMVMPH
                     VSLRAVGPLADGPQTLVDALIEAVGPTGNILAFVSWRDSPYEQTLGHDAPPAAIAQSW
                     PAFDPDHAPAYPGFGAINEFIRTYPGCRRSAHPDASMAAIGPDAAWLVAPHEMGAAYG
                     PRSPIARFLAHAGKILSIGAGPDAVTALHYAEAVARIEGKRRVTYSMPLLREGKRVWV
                     TTSDWDSNGILDEYAAPDGPDAVERIARDYLARTRVAQGPVGGAQSRLIDAADIVSFG
                     IEWLEARHAAPAAAALKPKQRRD"
     gene            complement(10879..11670)
                     /locus_tag="RHV13_003674"
     CDS             complement(10879..11670)
                     /locus_tag="RHV13_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA25"
                     /protein_id="HDV1243163.1"
                     /translation="MRVAVTIEISNQLSEVLSVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT         2170 a         3663 c         3625 g         2425 t
ORIGIN      
        1 taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg
       61 tgttatgagc catattcaac gggaaacgtc ttgctcgagg ccgcgattaa attccaacct
      121 ggatgctgat ttatatgggt atagatgggc tcgcgataat gtcgggcaat caggtgcgac
      181 aatctatcga ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg
      241 tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga cggcatttat
      301 gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac
      361 tgcgatcccc gggaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa
      421 tattgttgat gcgctggcag cgttcctgcg ccggttgcat tcgattcctg tttgtaattg
      481 tccttttaac agcgatcgcg catttcgtct cgctcaggcg caatcacgaa tgaataacgg
      541 tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg
      601 gaaagaaatg cataagcttt tgccattctc accggattca gtcgtcactc atggtgattt
      661 ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg
      721 agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt
      781 ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa
      841 taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg ttaattggtt
      901 gtaacactgg cagagcatta cgctgacttg acgggacggc gcttccctac cctcatggcc
      961 gcctcccagg aatcgaccca ggtgcttatc gtggacataa tcctttactc ataaatttat
     1021 cggctctgtt gcaaagattg gcggcagtca gaggtaggct gtcgctctgc gccgatcagg
     1081 cggctgctgc gaaatggtgg ttgagcatgc ccatggcctc cgtcagcgcc gagggcccaa
     1141 tgccaaaagc tctctccaca aggcgcacct cgcccctgat gccgggctgc aggcaccagg
     1201 ggcgagcctg tcctttgcgc agggctcgca tgacttcgaa tcccttgatc gtggcatagg
     1261 ccgtggggat cgatttgaaa ccgcgcaccg gcttgatcag tatcttgagc tttccgtgat
     1321 cggcctcgat cacgttattg agatacttca cctgccggtg ggccgtctcc cggtccagct
     1381 ttccttcgcg cttcaattcg gtgatcgctg caccatagct cggcgctttg tcggtattga
     1441 gcgtggcagg cttttcccag tgcttcaggc ctcgcagggc cttgcccagg aaccgcttcg
     1501 ctgccttggc gctgcgggtc ggcgacaggt agaaatcgat cgtgtcgccc cgcttgtcga
     1561 ctgcccggta caggtaggtc cacttgcccc gcaccttgac gtaggtttca tccaggcgcc
     1621 agctcggatc aaagccacgc cgccagaacc agcgcagccg cttctccatc tccggggcgt
     1681 agcactggac ccagcgatag atcgtcgtat ggtcgaccga aatgccgcgt tccgccagca
     1741 tttcctcaag gtcgcgatag ctgatcggat agcgacaata ccagcgcacc gcccacagga
     1801 tcacatcacc ctggaaatgg cgccacttga aatccgtcat cgttccgtcc gtccaatctc
     1861 cgccaagcat gctcaagctt cacgattttt gcaacagagc ccacacgagt attgagcata
     1921 gtcgagattg gtgcagatca cttctgatat tgaactgtca ggagctggct gcacaacagc
     1981 cattacgccc aatcaactgg tgcagtcgtc ttctgaaaat gacatttggt atctctcata
     2041 aacggatgtt tttgagagaa ctatcttcgg ccttcacacg cacgaaaggc ggcgaagctc
     2101 cgccgttaat ccgtccgccg gagatctcgc ccaggcaggc tgaaggccga gcaagcctga
     2161 caggcccgaa aagcccggca cgggcgtcgg cggcgatgac ggcggcggca ttatccaggg
     2221 ttgatgatgg aagtggagga tatcgacaac ctctcgcgca accaagacat cgcggtcgga
     2281 ctgcaagtga tcttgaagcc acgggcccgt cccaccccga catggacctc gatgcccgaa
     2341 cggacgttag atttcgagtt ctaggcgttc tgcgatgaag gttggatccc agccgggatt
     2401 gaaagtgtcg acgtgggtga atccgagccg ctcgtatagg ccacgcaggt tcgggtggca
     2461 gtcgagccgc agcttggcgc acccctgcgt tcgcgcggca tggcggcaag cctcgatcag
     2521 cgcggagctg acaccccggc ccgcatgtgt ccgtcgcacc gcgagcttgt gcagatatgc
     2581 ggcctccccc ttgagggcgt cgggccagaa ctcgggatcc tcggccgaca aggtgcaaca
     2641 gccgacgatg ccgtcgctgc aactcgcgac taggagctcg gatctcagga cgaaggtctc
     2701 cgcgaatgtc cggtcgatcc gcgcgacgtc ccaggcgggc gttcccttgg cggacatcca
     2761 cgccgcagcg tcgtgcatca gccgcacaac ctcgtcgata tcacccgagc aggcgacccg
     2821 aacgttcgga ggctcctcgc tgtccattcg ctcccctggc gcggtatgaa ccgccgcctc
     2881 atagtgcagt ttgatcctga cgagcccagc atgtctgcgc ccaccttcgc ggaacctgac
     2941 cagggtccgc tagcgggcgg cggaaggtga atgctaggca tgatctaacc ctcggtctct
     3001 ggcgtcgcga ctgcgaaatt tcgcgagggt ttccgagaag gtgattgcgc ttcgcagatc
     3061 ttcaggcgcg tgggtgcgga cgtagtcagc gccattgccg atcgcgtgaa gttccgccgc
     3121 aaggctcgct ggacccagat cctttacagg aaggccaacg gtggcgccca agaaggattt
     3181 ccgcgacacc gagaccaata gcggaagccc caacgccgac ttcagctttt gaaggttcga
     3241 cagcacgtgc agcgatgttt ccggtgcggg gctcaagaaa aatcccatcc ccggatcgag
     3301 gatgagccgg tcggcagcga ccccgctccg tcgcaaggcg gaaacccgcg cctcgaagaa
     3361 ccgcacaatc tcgtcgagcg cgtcttcggg tcgaaggtga ccggtgcggg tggcgatgcc
     3421 atcccgctgc gctgagtgca taaccaccag cctgcagtcc gcctcagcaa tatcgggata
     3481 gagcgcaggg tcaggaaatc cttggatatc gttcaggtag cccacgccgc gcttgagcgc
     3541 atagcgctgg gtttccggtt ggaagctgtc gattgaaaca cggtgcatct gatcggacag
     3601 ggcgtctaag agcggcgcaa tacgtctgat ctcatcggcc ggcgatacag gcctcgcgtc
     3661 cggatggctg gcggccggtc cgacatccac gacgtctgat ccgactcgca gcatttcgat
     3721 cgccgcggtg acagcgccgg cggggtctag ccgccggctc tcatcgaaga aggagtcctc
     3781 ggtgagattc agaatgccga acaccgtcac catggcgtcg gcctccgcag cgacttccac
     3841 gatggggatc gggcgagcaa aaaggcagca attatgagcc ccatacctac aaagccccac
     3901 gcatcaagct tttgcccatg aagcaaccag gcaatggctg taattatgac gacgccgagt
     3961 cccgaccaga ctgcataagc aacaccgaca gggatggatt tcagaaccag agaaagaaaa
     4021 taaaatgcga tgccataacc gattatgaca acggcggaag gggcaagctt agtaaagccc
     4081 tcgctagatt ttaatgcgga tgttgcgatt acttcgccaa ctattgcgat aacaagaaaa
     4141 agccagcctt tcatgatata tctcccaatt tgtgtagggc ttattatgca cgcttaaaaa
     4201 taataaaagc agacttgacc tgatagtttg gctgtgagca attatgtgct tagtgcatct
     4261 aactttgtgg gtataggaag ttcaaccgcc cttttttgcg agtctggcag gtaggaagcg
     4321 ggggcgtgtc cgattttttg tgtgtgaggc ggtttagtcc ggaagtctct ttcagcgagc
     4381 cagcgtgaat cggtcttcgt agattatcgc gaactggttc atcgccgcct tccagtgctg
     4441 agacgcgcgg ctccactcgg cggtgatgtt ccgcagcgcc agccagatca gcttggtggc
     4501 ggcctcgtcg ctggggaagt ggccgcgggt cttgatgatc ttgcgcaggc gcgcatgcac
     4561 gctctcgatt gcgttggtcg tgtagatcac ccggcgcacg tccggcggga acgcgaagaa
     4621 cggcaccacg cgcgtccagg cacggcgcca ggcggcgacc accgtcggga agcgctcgcc
     4681 ccagtcgccg gcctcgaacg catccagctc ggcctctgcg gcctcggcgg tcggggccgt
     4741 gtagatcggc ttgatcgccg ccgccagcgc cttgcggtcc ttccagcccg catagtccag
     4801 gctgttgcgg atcaggtgca cgatgcaggt ctgcagcgtg gtcgccggga acaccgcttc
     4861 cagcgcctcc gggaagccct tcaggccgtc agccacgccg atcaggatgt cgttgacgcc
     4921 gcgcgtcttg aggtcgttga acaccttcat ccagaacttc gcgccctcgg tgttctcgat
     4981 ccacagcccg aggatgtcgc gggtgccgtc ggccagcacg cccagcgcca gatacaccgc
     5041 cttgttgcgc accacgccgt cctcgcggat cttcacccgc agcgcgtcga agaacaccac
     5101 cggatacatc gcttccagcg gccgcgcctg ccatgcggtg acctcggcca tcacctcgtc
     5161 ggtgaccttg ctgatgaact ccggcgacac ctcgacgctg tacatctcgg ccaggtgccc
     5221 ctggatctca cgcaccgtca tgccgcgggc gtacatcgag acgatccgat cgtcgaaccc
     5281 ggcgaagcga cgctcgtgct tggggatcag ctgcggctcg aacgtacccg tccggtcgcg
     5341 cggcacgtcg atgcgcagcg gaccctcgtc ggtcagcacc gtcttgccgc tgcggccgtt
     5401 gcggtggttg ccgctcccgc cctcggcgtc ggccaggtgc aggccgagct ccgcgcccag
     5461 cgcccgctcg atcacggcct tcttcagctg ctggaacacg gcttcgaccc cggccgcgtc
     5521 catcggaccc gggatcagct tctcgagaaa ttccttcggc agatcaatgg ccggcgtgac
     5581 caccggcgtc ttcttcttgc gtggcataca tgctccttgg cagcatcatg gtatgcctct
     5641 cacacaaaat ttctgacacc ctcggaagcg gccgcgcaag cgccgctttg ggcacagatc
     5701 gggcacagat cgggggttca tgcgtgggtt ttcgacatcg cggggccacg cagcggctca
     5761 gtgcgtagcc atttctcacc gaaacccggc acagggccga gcgcagccct gcggggtcgt
     5821 ggccgcatcg gatcggactc tggcttggct gccggctgtg tggccggcac ctcgtcacgc
     5881 ggccggcggc ggcgcgcgcg ctgcgggccg gtagtgcgct tgctccagcg cgccgtgttt
     5941 ctcgaagtgg gcgaggatgg cgcgaatggc cttggggtct tcgatgctgg cgacgatccg
     6001 caccgcgccg ccgcagtggg cacaggtggt gacgtcgatg gaaaagaccc gcttgagccg
     6061 ttgcgcccag ctcatcgcac ggcgcttctg ctcggggctg cgcggctcgt cgtgggcgct
     6121 tgcgtcaacc ggcaccgcat cgcccgcagg ccgcctgccg cgccccgagg gcgtcagctg
     6181 cgcacgcagg tttgcattcg gggcgaatac gccgtggaag cgggtgagat gcgcgcgagg
     6241 tggcgggacc agtgccgcca gcttggcgat gaagtccacc gcatcccatt cgacatgcgt
     6301 ggtcccattt cgccacggcg tcttgagctg ataccgcacc ctaccctgcg gtgagatcga
     6361 cagccgctgc tcgctgatcg ccgggcgcgt gatgtagcgg cacagctttt cgagcttgtg
     6421 gctttcgtgt gcttccgcgg ccacgccggc atgcagcgag aagccgccga ccttgccggc
     6481 gtcgccctcc agcgagcctg cgtcaccggg caatatttgc agcgtgacga ccttgcggcc
     6541 agcgtcgcga ccggtggcga tgcggtaggt catcgaactc atccgcagcc catccatgcc
     6601 gtcgtcgcta cccgcgctgt cggacaggaa cacggattcg tcttcgcctt cgagccagcc
     6661 gcggcgcgac aggtgccggc acacgcgatg cgcgatggtg ccggccagtt ccgtcagttg
     6721 cgccgatgtg ggcgcacggg tgcggtgcag gcgcggcttg cgctgcggac gctcggtggt
     6781 gtcctcgtac acgccgtcga gccacagcat gtggaagtga acattcaggt tcagcgcgct
     6841 gccgaagcgc tggatcaggg tcaccgcgcc gcattgcgcg ctggcgtggt cgacgccggc
     6901 ttgatcggcc aaccagccgg cgatcacacg atgcacgatg cccagcaccg ggccgatggc
     6961 ctccggcttg ctggcgaaca ggaagcgcag cgggtacgga aaactcagca cccattgccg
     7021 caccggccgc gggccaaaca cctcctccac caggtgccgc gcgctctcgg ccatgcgccg
     7081 cgccccgcag ctggggcaga agccgcgctt cttgcaggag aaggccacca gcctctcggc
     7141 ccggcactgc tcgcacacca cgcgcaggaa accgtgctcg agcacgccgc aacgcaggta
     7201 ggtctcgaac tcctcgcgca catactcggg cagcgggcgg tcttcggcct caagacgcgc
     7261 gatgaagtcc gggtagtgcg cctgcactag cgcgtacagc agcgtgcgct cgggcaggtg
     7321 gcgcgcgtaa cgcgcagcgg cgtgggcggc cggcagtggc gcgcacacct cggcctgccg
     7381 ccggggtgcg gtcaggcgcg gcacgcagcg ctccgttgca gggacggctg ctcagggttg
     7441 cgcccgcgag cacgcgttca tatcggcgtt ttctgatctt cgcgtcagac attgccgcgg
     7501 ggcgggtagg aagtctcccg cctcggacgc cagaacgcac aaagcccggc acgaggccgg
     7561 gctttgtggt cttgcttgat ggatcgcggg gcttagaaat ccatgccgcc catgccgccc
     7621 atgccgccgc ccggagcggc cggctcttcc ttcttcggcg cttcggccac catcgcttcg
     7681 gtggtgatca tcaggccggc gatggacgcc gcgttctgca gcgcggagcg cgtgaccttg
     7741 gtcgggtcca ggatgccgaa ctcgatcatg tcgccgaact cgccgttggc ggcgttgtag
     7801 ccgaacgcgc cggtgccttc ggcgacgcgg ttcagcacca cgctcggctc gtcgccggcg
     7861 ttggtgacga tctcgcgcag cggggcttcc atcgcgcgca gggcgatcgc gatgccgtgg
     7921 ttctggtcct cgttggcgcc cttcagctcg gcgatcgcgg ccttggcacg gatcagggcg
     7981 acgccgccgc ccgggacgat gccttcctcg accgccgcac gggtggcatg cagggcgtct
     8041 tcgacgcgcg ccttcttttc cttcatctcg acttcggtgg cggcgccgac cttgatcacc
     8101 gcaacgcccc cggccagctt ggccacgcgc tcctgcagct tctcgcggtc gtagtccgag
     8161 gtggtctcct cgatctgcgc cttgatctgc ttgatgcggg cttcgatgtc cgcggtgtcg
     8221 ccggcgccgt cgatgatggt ggtgttctcc ttcgagacct gcaccttctt cgcgcggccg
     8281 aggtcgttga tggtcgcctt ctcgagcgag aggccgactt cctcggagat cacggtgcca
     8341 ccggtgagga tggccatgtc ctccagcatc gccttgcggc ggtcgccgaa gcccggggcc
     8401 ttgaccgcgc agaccttcac gatgccgcgg atggtgttga ccaccagcgt ggccagcgcc
     8461 tcgccctcga cgtcctcggc gacgatcagc agcggcttgc cggccttggc cacgccctcg
     8521 aggatcggca gcaggtcgcg gacgttcgag atcttcttgt cgtgcagcag gatgaacgga
     8581 tcctccagct cggcctgcat cgactgcggg ttgttgatga agtacggcga caggtagccg
     8641 cggtcgaact gcatgccctc gacgacgtcg agctcgttct ccaggcccga gccctcctcg
     8701 acggtgatca cgccttcctt gccgaccttg tccatggcct tggcgatcag ctcgccgatg
     8761 tcggtgtcgg agttggccga gatcgaaccg acctgggcga tctccttgct ggtcgagcag
     8821 ggcttggaga tcttcttcag ctcctcgacg gccgaggtga cggccttgtc gatgccgcgc
     8881 ttgaggtcca tcgggttcat gccggcggcc acggccttca tgccctcgcg gatcagggcc
     8941 tgggccagca cggtggcggt ggtggtgccg tcgccggcgt tgtcggaggt cttggaagcg
     9001 acttccttga ccatctgcgc gcccatgttc tcgaacttgt cggccagttc gatctccttg
     9061 gcgacggaca cgccgtcctt ggtgatcgtc ggggcgccga agctcttctc gagcacgacg
     9121 ttgcggccct tcgggcccag ggtcgccttg acggcgttgg cgagcacgtt cacgccgcgg
     9181 accatgcggg cgcgggcgtc ttcaccgaaa cgaatatcct tggcagccat tgcggaatta
     9241 cctcagttct gtgattgcgg gttgggatgc ggcgaagcgg cgggctcagc ccagcacggc
     9301 gaagatgtcg tcttccttca ccaccaggta ctcggtgccg tcgagcttga cctcggtgcc
     9361 ggcgtacttg ccgaacagca cctggtcgcc gaccttgacc ttcggcgcgc gcacgttgcc
     9421 gttgtcgaac accttgccct cgcccacggc gacgacctgg cccttgatcg gcttctcggt
     9481 ggccgaatcc gggatcacga taccaccggc ggagaccttc tcttcctcgg tgcgcttgat
     9541 gacgacgcgg tcgtacagcg gcttgatgtt aatcgccgcg catatcttcc tcgtggggct
     9601 acccatcgcc tttatcgcgc gccgccgcga ccgtgcgcgc gcgcaaaaat tcgccgcctg
     9661 atcgcctcgg gcaatcatcc ttgtcgaggg tgccaagctg ttctttactg aagaacgtcc
     9721 cgacatcctt gttgaagagg cggcgggctt gtgggcctga tgctatgcgg ctgggcgaga
     9781 gggaggaata tatgtccgga ataatcgacg actcagtcgc ggcgttgttt cggcttcagc
     9841 gctgccgccg ctggcgcggc gtggcgcgcc tcgagccatt cgatgccgaa ggaaacgata
     9901 tcggccgcgt cgatcagccg ggattgcgcg ccgccgaccg ggccttgcgc aaccctggtg
     9961 cgggcgagat agtcgcgggc gatccgttcg accgcgtcgg ggccgtcggg cgcggcatat
    10021 tcgtcgagga tgccgttcga atcccagtcg gacgtggtga cccagacgcg cttgccttcg
    10081 cgcagtaagg gcatcgaata agtgacgcgg cgcttgccct cgatccgcgc caccgcttcg
    10141 gcataatgga gcgcggtgac tgcatcgggc ccggcgccga tcgacaggat ttttcctgcg
    10201 tgggcgagaa aacgcgcgat cggcgagcgg gggccataag cggcgcccat ctcgtgcggc
    10261 gccaccagcc acgccgcatc gggcccgatc gccgccatcg atgcgtcggg atgggcgctg
    10321 cgccgacacc ccggataggt tcggataaat tcgttgatcg cgccaaagcc ggggtaggcg
    10381 ggcgcatggt cggggtcgaa cgcaggccag ctttgggcga tggcggcggg cggcgcatca
    10441 tgacccagcg tctgttcata gggcgaatcg cgccacgaga cgaaggcgag aatattcccg
    10501 gtggggccga cggcctcgat cagcgcatcg acaagtgtct gcggtccgtc cgccagcggc
    10561 ccgacggcgc gcaacgacac atgcggcatc accatatcgc ctgagcgcac gccgaggtcg
    10621 cgcaattgcc ggaccagctc gcttttggac cagggcgtcg cgggtgccgt cgcgggttcg
    10681 tgcaaatcgc cgtttttgcg gggatcagtc atcatgatat atctcccaat ttgtgtaggg
    10741 cttattatgc acgcttaaaa ataataaaag cagacttgac ctgatagttt ggctgtgagc
    10801 aattatgtgc ttagtgcatc taacgcttga gttaagccgc gccgcgaagc ggcgtcggct
    10861 tgaacgaatt gttagacatt atttgccgac taccttggtg atctcgcctt tcacgtagtg
    10921 aacaaattct tccaactgat ctgcgcggga ggccaagcga tcttcttctt gtccaagata
    10981 agcctgtcta gcttcaagta tgacgggctg atactgggcc ggcaggcgct ccattgccca
    11041 gtcggcagcg acatccttcg gcgcgatttt gccggttact gcgctgtacc aaatgcggga
    11101 caacgtaagc actacatttc gctcatcgcc agcccagtcg ggcggcgagt tccatagcgt
    11161 taaggtttca tttagcgcct caaatagatc ctgttcagga accggatcaa agagttcctc
    11221 cgccgctgga cctaccaagg caacgctatg ttctcttgct tttgtcagca agatagccag
    11281 atcaatgtcg atcgtggctg gctcgaagat acctgcaaga atgtcattgc gctgccattc
    11341 tccaaattgc agttcgcgct tagctggata acgccacgga atgatgtcgt cgtgcacaac
    11401 aatggtgact tctacagcgc ggagaatctc gctctctcca ggggaagccg aagtttccaa
    11461 aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag
    11521 caaatcaata tcactgtgtg gcttcaggcc gccatccact gcggagccgt acaaatgtac
    11581 ggccagcaac gtcggttcga gatggcgctc aatgacgctt agcacctctg atagttggtt
    11641 cgaaatttcg atggtcaccg ctaccctcat gatgtctaac tttgttttag ggcgactgcc
    11701 ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc
    11761 ttgctgcttg gatgcccgag gcatagactg tacaaaaaaa cagtcataac aagccatgaa
    11821 aaccgccact gcgccgttac caccgctgcg ttcggtcaag gttctggacc agttgcgtga
    11881 gcg
//
LOCUS       DAPGOF010000153        11806 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.154, whole genome shotgun sequence.
ACCESSION   DAPGOF010000153 DAPGOF010000000
VERSION     DAPGOF010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.154"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(5..940)
                     /gene="rihA"
                     /locus_tag="RHV13_003675"
     CDS             complement(5..940)
                     /gene="rihA"
                     /locus_tag="RHV13_003675"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HDV1243164.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPAFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNAIVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(1058..1783)
                     /gene="gltL"
                     /locus_tag="RHV13_003676"
     CDS             complement(1058..1783)
                     /gene="gltL"
                     /locus_tag="RHV13_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HDV1243165.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            complement(1783..2457)
                     /gene="gltK"
                     /locus_tag="RHV13_003677"
     CDS             complement(1783..2457)
                     /gene="gltK"
                     /locus_tag="RHV13_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HDV1243166.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            complement(2457..3197)
                     /gene="gltJ"
                     /locus_tag="RHV13_003678"
     CDS             complement(2457..3197)
                     /gene="gltJ"
                     /locus_tag="RHV13_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HDV1243167.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            complement(3367..4275)
                     /gene="gltI"
                     /locus_tag="RHV13_003679"
     CDS             complement(3367..4275)
                     /gene="gltI"
                     /locus_tag="RHV13_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HDV1243168.1"
                     /translation="MQLRKPATAILALALSAGLAQADDTAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            complement(4672..6210)
                     /gene="lnt"
                     /locus_tag="RHV13_003680"
     CDS             complement(4672..6210)
                     /gene="lnt"
                     /locus_tag="RHV13_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HDV1243169.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            complement(6235..7113)
                     /gene="corC"
                     /locus_tag="RHV13_003681"
     CDS             complement(6235..7113)
                     /gene="corC"
                     /locus_tag="RHV13_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HDV1243170.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            complement(7203..7670)
                     /gene="ybeY"
                     /locus_tag="RHV13_003682"
     CDS             complement(7203..7670)
                     /gene="ybeY"
                     /locus_tag="RHV13_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDV1243171.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            complement(7667..8707)
                     /locus_tag="RHV13_003683"
     CDS             complement(7667..8707)
                     /locus_tag="RHV13_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDV1243172.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            complement(8860..10284)
                     /gene="miaB"
                     /locus_tag="RHV13_003684"
     CDS             complement(8860..10284)
                     /gene="miaB"
                     /locus_tag="RHV13_003684"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HDV1243173.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            10430..11605
                     /gene="ubiF"
                     /locus_tag="RHV13_003685"
     CDS             10430..11605
                     /gene="ubiF"
                     /locus_tag="RHV13_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HDV1243174.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFIA
                     DSQPDVRISAISAASVSLLKGLGVWDAAQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRHDDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMVQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
BASE COUNT         2799 a         3097 c         3097 g         2813 t
ORIGIN      
        1 aatgttaagc gtaaaatttc agacgatcag ccagtaaatc aacaaagccc tgacgatcaa
       61 catcgaccat tacaatggca ttcggtttat tgccggtcag ataataataa tcaacaaccg
      121 tcataccctg ggtatatttc ccttgtgttt ccacgccaac ccagcgctca acagtggtaa
      181 acaactccgg tttcaacagc caggcgatgg tgcatgggtc atgcagcggt gcgccgacaa
      241 agccccattt ttcgtcttta tgatattcga ggaagaaatc cagcagttcg gcaacaatgg
      301 ttgaaacagg gttgccaatc gcgcggaaac gctcggtgtc ttcaacgtgg atttgtgctt
      361 tgtgagtaac atccagaccg gccatcacca ccgggatccc tgactggaag acaatttctg
      421 ccgcttccgg gtcaacgtaa atattaaatt cagccgcagg cgtccagtta cccagcccca
      481 tagcgccacc cataatcacg atacgggcaa ttttgctatg cagttccgga tggctattga
      541 gcagcaaggc aacgttagtt tgcggtccgg tagacacaat cgtgacaggt tccgcacttt
      601 cacgcagcgt tttcgccatt agctctaccg ccgtacagtt ttgcggtgcg aacgccggtt
      661 ccggtaatgc cgggccgtcg agaccgcttt cgccgtgcac attgtccgcg ataattaact
      721 cacgcattaa cggttttacc gcgccgcctg ccaccggaat atcggtgcga ttaagcaagg
      781 tcagcatacg cagaacattg cgtaaggttt tttctggtgt ctggtttccg gcggaagacg
      841 taattgcttt gacatcaagc tctggtgagg cgagggcgag aactattgcg atagcgtcgt
      901 catgacctgg gtcgcaatct aacagaattg gcagtgccat tgttgctcct tgttgtgtcc
      961 ttctttgcga caagggtaac gccaggatgt aacagatacg aggggcgaaa cgataaagcg
     1021 tgagatggcg cgcaattggg gatgcgcgcc agagtgatta atgcaggatt ttcgcgagga
     1081 agtcttttgc gcggtccgat ttcggatcat cgaagaacgc atctttcggc gagtcttcaa
     1141 caattttacc ctcatccata aagatcaccc gattcgccac tttgcgggca aagcccattt
     1201 cgtgggtcac caccatcatg gtcattcctt cgttcgccag ttcgaccatt acgtccagca
     1261 cttcgttgat catctccgga tccagcgccg atgtcggttc gtcaaacagc atggcaatag
     1321 gatccataca caatgcgcga gcgatcgcca cacgctgctg ctgaccgccg gaaagctgcg
     1381 ccggaaattt attggcatgt gcggaaagtc ccacgcgctc cagcaatttc aatgcttttt
     1441 cacgagccgg cgctttatcg cgtttaagca ctttcacctg cgccagggtc aggttttcga
     1501 taatcgacag atgagggaac agctcaaaat gctggaatac catcccgacg cgggaacgca
     1561 gctttgccag atcggttttc ttgtcgttaa ccacgatacc atcgacggtg atttcacctt
     1621 gctgcactgg ttcgaggccg ttgacggttt taatcagcgt tgatttgccg gaaccagacg
     1681 ggccgcaaac caccaccact tcgccttttt tcacttcggt tgagcagtcg gtcagcacct
     1741 gaaagtgacc ataccatttt gaaacatttt tcagggtaat cattatgctg tccttctttt
     1801 caagtagctg accaacaacg acgcgctaag gctaataacg aaataaacaa atccggcaaa
     1861 caggatcatc tcaacctgcg taccatcacg ctcaccaatg gttgaggcgg tacggaagaa
     1921 atcggccaga gataacacat acaccagtga ggtatcctgg aacagtacga tgccctgagt
     1981 gagcagcagc ggcaccatcg cgcggaacgc ctgcggcaga ataatcagtt tcatcgactg
     2041 ccagtgagtc attcccaacg ccagcgcggc gctcgactga ccacgagaaa tactttgaat
     2101 accggcacgg ataatctctg aatagtaggc cgcttcaaac atcgaaaacg ccaccatcgc
     2161 cgaaattaaa cggatatcat tttttggcga taatcccagc acgttttgca gaaaacccgg
     2221 cacgatcagg taaaaccaca gcaaaaccat aactaaagga atcgagcgga atacgttaac
     2281 gtaggctttg gcaaaccacg ctacgggcgc aaagctggac aagcgcatca ccgccagcat
     2341 ggtgccccac aaaataccaa tcacgactgc cgtgacggtg attttcaggg tgatcaccag
     2401 cccgtcgagc agatatggca gggaaggaac aatggaactc cagtcaaact cgtacattat
     2461 ttgcccccca tgttgccggg caggcgaact ttacgttcaa ccagcgtcat caccagcatg
     2521 ataaaagcgt taatcaatac atacgccagc gtaatggcgg taaacgactc ccaggcgtga
     2581 gccgagtagt cgagcaattt acccgcctgc gctgccatat ctaccagacc gatggtcgag
     2641 gcgatggcgg agtttttcac caggttcatc atctctgagg tcatcggcgg gacgataacg
     2701 cgataagcat taggcagcag tacgtagcgg taagcctgcg gtagcgtcag acccatcgcc
     2761 agcgcggcat tcttctgccc acgcggcagc gactgaatag cggcgcgtac ctgttcgcaa
     2821 acacgggcgg cggtaaacag ccccaggcag agcatggagg agaggaaaaa ctgaatattg
     2881 ggatccagct cggccttaaa ccacatgccg attttctccg gcaacagctc cgggatcacc
     2941 agataccagg taaagaattg cacaatcaac ggcacgttgc ggaacagttc gacatacaac
     3001 gtacccagac cggagagaaa acggtttgga acggtacgta aaatgccgaa aaatgaaccg
     3061 acgaggaaag cgataatcca ggcgcagatc gacaaagcga tcgtcacctg aaaaccactc
     3121 cagatccaac caagataggt ggtgttgccg aacggggctt gttgtaaaaa aataccccag
     3181 ttccagtcta tagacataaa tctactccag aaaaaagagg gtagcagcgt taactgctac
     3241 cctcgaagat tgttacccag cgtattgcgg ttttcaggcc cgatggggaa cgaccatcag
     3301 gcgtatagtc tgtccgtgct acgtaacaat cgagagggct agaatttccg cccctggttc
     3361 ttgtaattag ttcagtgctt tgtcattcgg ttctttgaac agtgctttca tttcgtctga
     3421 cagttcgaaa ttcatgttca ggtttttcgg cggaatggga tttttgaacc atttatcaaa
     3481 ccatttttcc gcttcaccgg aggtctgcac ctgagcgata gtgtcatcca tcagcttttt
     3541 gaactgcgga tcgtctttac gcaacatgca accataggct tcctgagact gcggcttgcc
     3601 gacgatatcc cagttgtctg gtttcttcgc tttcgcacgt tcaccggcca gcagagcatc
     3661 atccatcata aaggcaacgg cacgaccgct ttccagggta cggaaagagt caccgtgatc
     3721 tttggcgctg ataattcgca tattcatttt ttgctcttca ttcagtttgt tgagcaaaac
     3781 ttcagaggta gtgccggaag tgacgactac ggctttgcct ttcagatcgg cgaaatcttt
     3841 gatatcgcca ccctttttgg tcaacaggcg cgtaccgacc acgaaaatag tgtcagagaa
     3901 agccgcctgt ttttggcgtt cgacgttgtt ggtggtagaa ccacattcaa aatcaaaagt
     3961 gccgttttgc agcagcggaa tacggttttg tgaggtaatc ggaatcagtt ttacctgcaa
     4021 atccggtttg ttgagtttct ttttcactgc ttcaacaatg gcgttggagt aatcctgcga
     4081 gtaacccacc actttttgct gattgtcgta ataagagaaa ggtactgaag attcacggtg
     4141 accgacgaca atcacaccgt ttttggcgat tttgtccagc gtactgcccg ctgccggggc
     4201 tgtgtcatct gcctgtgcca gtcctgcgga aagcgccagg gccaggattg ctgtggcagg
     4261 tttacgtaat tgcatatcca actcctttat cttctgcgtt aagaacgcat ggatacccgt
     4321 tgtgagtgtt tgtgttgtta tcatctgcaa ctttattgtg cagtgttgtg cctgttaggt
     4381 aacatttagt ttggctaaat gtaaagatat tgctgtttta ttgtttatat ttgggaggtg
     4441 cgccgcacca ttccgaagca aaattcttaa aatgcactct tttggtgcta ccggtggatt
     4501 gctgtggtgc aaccgggttg tactgatgct ggttcaagct ttgccttgta taacaaagca
     4561 atagatgtgc caaagttgga taggagaata ttattatccg gataacgcac tgatgccgca
     4621 tccggtgagc gtgccgaaat atgggatgta ttccggcacg ctaagaaggg attatttacg
     4681 tcgctgacgc agactcatca acacagcggc aaaaccaaac agtgccgtca gcacccacag
     4741 cggccagttg ccggtacgtg cgtatggtgt gagtccggtg gtcggcgtca cgttagtggt
     4801 caacacttcg cgggtgaact gagggatcat cgcctgaatc tcaccctgcg gaccaatcac
     4861 cgccgtaatg ccgttgttgg tactacgcag caatgggcgc gccagctcca acgcacgcat
     4921 acgcgccatc tggaagtgct gccatggacc aatagactta ccaaaccatg catcgttgga
     4981 gatagtcagc aggtagtcgg tatccgggcg gaagttatca cgcacttgct cgccgagaat
     5041 gatctcgtag caaatagccg cagtaagctc aataccattt accgacagcg gcggctggat
     5101 atatggccca cggctgaacg acgacatcgg cagatcaaag aacggtgcta acggacgcag
     5161 aatcgactcc agcgggacaa actcgccaaa cggcaccaga tggtttttgt tatagcgatc
     5221 ggctgattcg tagctgtacg gtgcaccttt acccagcgtg atgatggtgt tgtaggtatc
     5281 gtagcggttc tgcttattga gacgcgcatc gacaatcccg gtgaccagcg agctaccttt
     5341 atcacgcaac tcaccgtcca gtgctttgag gaacggttgc tgattaattt ccagatcggt
     5401 tatcgccgac tccggccaga taatcaacga tgatttgccc atcagcggtg ccgttgcgtt
     5461 gtagtaaatc ttcagcgtat taagaagctg gccttcgtcc catttcagcg attgcggaat
     5521 atcgccctga accatcgaaa cctgaatggt tttctccggt tgcggggtaa accattggat
     5581 gtaacgcagc gggaagggta gggcaaacag cacgacggcc actaccagcg gacgccagtt
     5641 gcgtttgacc aacgccagtg ccagcaggcc actaaccatc atcagcagga agttaatggc
     5701 ttccacgccc attatcggtg ccagcccttt taacgggccg tcaatctggc tatagccgaa
     5761 ctgtaaccac gggaagcctg tcagtaccca accgcgcaga aactcggtca cttgccagag
     5821 agcaggggcg gcaatcgcca cgcgcagcca ggtggttttc ggccacagac gcgacagcac
     5881 gccagcaaac agtccggtat acagcgacaa atacgccgcc agcagtacca ccaggaagat
     5941 gttaaccggg ccaggcattc cgccaaaggt cgcgatgctg acatagaccc agttaatacc
     6001 gctgccaaag aggccaaatc cccagcaaaa gccaatagcg gcagactgga gtggacggcg
     6061 attaaaggtc agcgcctgaa gccccatcag cgaaataatc gccgcaggcc agacgtcgta
     6121 aggagagaag gccagcgttc cgcaggcacc gaataataac gccagcagca ggcgaatgcg
     6181 ctggcgttca attaatgagg caaaagccat gtagttatct atccagtttc ggttttattc
     6241 atccagcttt ggctggggtg agtcatccgg gattttgaca tgaacctgaa taatacgccg
     6301 actgtcggcc atcgccactt tgaactggta accgtcgatg tcgatggttt caccacgcgc
     6361 tggaagatgc ccaaacgcct gcatcaccag accaccgata gtgtcgacct cttcatcgct
     6421 aaagtgggtg ccgaacgctt cgttgaagtc ttcaatggaa gccagcgcgc gcacggtcca
     6481 ggtatgacga ctcagctgac ggaagtcgat atcatcttct tcgtcatact cgtcttcaat
     6541 ctcaccaaca atcagttcca ggatgtcttc aatggtcacc agaccggaaa ccccaccgaa
     6601 ttcgtcaatg acgatcgcca tgtggtaacg ctgagagcga aactctttca gcatccggtc
     6661 tacgcgctta ctttcaggaa cgacaaccgc ctgacgtaac actttgtcca tgctgaaggc
     6721 ttcagcatcg ctgcgcataa acggcagcaa gtctttcgcc atcagaatcc cttcaatgtg
     6781 atctttgtct tcgctaatca ccgggaaacg tgagtgggcg gactcgatga tgacatcaag
     6841 gcattcgtcc agcgtctggt tgcgtttcag ggtaatcatc tgggagcggg ggatcatgat
     6901 gtcgcggacg cgttggtctg cgatgtccat caccccttcg agcatatcgc gcgtatcttc
     6961 gtcgataagg tcgttctgcc cggaatcacg gatcagcgcc agcagttcgt cacggttttt
     7021 cggttcaccg tggaaaagtt ggctgagtaa cagggagaaa aatcccttct tgttgcttat
     7081 cgtgtcacta ctgtgtgaat tgtcgtcgct catggcgtcg tatgggttct catgttagtt
     7141 aatcaaaacg ccgtcgttaa tcaccaacgg cggggacgtc tgccagtcaa atgcctggca
     7201 aattattctt tctcggcaat gtacggatcc tcatagccca gagcaagcat aatctctgtt
     7261 tcgagggctt ccatttcttc tgcttcgtca tcttcgatgt gatcgtaacc taacaaatgc
     7321 agactgccgt gcaccaccat atgcgcccag tgcgcctcca gtggtttgcc ttgctcctgt
     7381 gcttccttct caaccacctg acggcagata accagatcgc ccagtagcga catctccatg
     7441 ccgggcggca cttcaaacgg gaaggagagc acgttggtcg gcttatcctt accgcgatag
     7501 gtcagattga gactgtggct ttcggcggta tcgaccacgc gaatcgtcac ttccgactct
     7561 tcctgaaact gcgggatcac cgcattcagc catgtttgaa actggctctc ttccggcaat
     7621 ccggagttat cttcacatgc cagttgtaaa tcgaggatca cctgactcat ttttgttcct
     7681 gttcttcgcg cttgcgttct gccgccagcg ccgctttacg tttttgttcg gcttcttccc
     7741 aggcttcata ggcgttaacg atacgcgcca ccaccgggtg acgaaccacg tcttcgctgt
     7801 ggaagaagtt aaagctgatc tcttcgacat cggccagcac ttcgatggca tgacgtaagc
     7861 ctgatttagt attacgcggc aggtcgatct gtgtgacgtc accggtgata accgcttttg
     7921 agttaaaacc gatacgggtc aggaacatct tcatctgttc gatggtggtg ttctggctct
     7981 catcgagaat gataaaggcg tcattcagcg tacgaccacg catataggcc agcggcgcga
     8041 cttcaataac gttgcgctca atcagtttct cgactttctc aaagcccagc atttcaaaca
     8101 gcgcgtcgta cagtgggcgc agatacgggt ctactttctg gcttaaatca ccaggcagga
     8161 agcccagttt ctcaccggct tctactgccg gacgagtcag cagaatacgg cgaatttcct
     8221 gacgctccag ggcatcaact gctgcagcca ctgccaggta ggttttaccc gtacccgccg
     8281 ggccaacgcc gaaggtaatg tcatggtcga gaatattggc gatgtactgc gcctggtttg
     8341 gcgtgcgcgg cttaattacg ccgcgtttgg ttttgatatt gaccgctttg ccgtactccg
     8401 gcacgctctc cgcgctttgc tccagtaccc ttgcttcttt aatcgcaagg tggatctgtt
     8461 ccggttcgat atcctgaatc tgaccgcgca tcggggcagt atcgacatac aggctacgca
     8521 gaatgtctgc cgcagcggtg acgcaaatcg gacggccggt cagtttaaag tggttatcgc
     8581 ggcgattgat ctcgatgccg agacggcgtt cgagctgctt gatgttgtca tcaaacgggc
     8641 cgcacaggct caacagacgc gcattgtctg ctggctccag ggtgatttcg cgagtgtcta
     8701 tgttcaaacc gttcctctta tctgtatgcc gccggaagct gaacattcac cggcctataa
     8761 ggaaattatt cacgccacag gaaaaaggcg caagcattgc gataaagatg gggataaaga
     8821 gagaaaaaac aaggcccacc ggaacggcgg gcctgagaat tacggctgat aataacccac
     8881 accaaggtcg ttttctttgc gagtacgggc aatcaccgat tctggtgttt ctgccacgcg
     8941 cagacccatt tcatcttcag ttcgcaccac tttaccgcgc agagagttcg ggtagacgtc
     9001 ggtaatttct acatcgacga atttaccgat catatccggc gtgccttcga agttgaccac
     9061 gcggttattt tccgtacgcc cggaaagctc catgatgctc ttacgcgatg taccttctac
     9121 cagaatacgc tgggtggtgc cgagcatccg gcggctccac gccatcgctt gctgattaat
     9181 gcgctcttgc agaatataca gacgctgctt cttctcttct tccggaacat catcaaccat
     9241 atcggcggct ggtgtacccg gacgtgcaga gaagataaag ctgtagctca tgtcgaaatt
     9301 gacgtcggca atcagcttca tcgttttctc gaagtcttcg gtggtttcgc cagggaagcc
     9361 aacgatgaaa tcagaactga tctgaatatc tggacgcgcc gcacgcagtt tacggatgat
     9421 cgctttgtac tccagcgccg tatgggtacg tcccatcagg ttcagaatgc gatcggaacc
     9481 gctctgtacc ggcaggtgca ggaagctcac cagctccggc gtgtcgcgat acacttcgat
     9541 gatatcgtcg gtgaattcga tcggatggct ggtggtaaag cgaatacgat cgatcccgtc
     9601 gatcgcagca accagacgca acagatcggc aaacgatccg gtggtgccgt cgtagttttc
     9661 accacgccag gcgttcacgt tctgaccgag caggttgact tcacgcacgc cctgagccgc
     9721 aagctgggca atctcaaaca gaatatcgtc ggacggacgg cttacctctt caccacgggt
     9781 gtaaggcacc acgcagtagg tgcaatattt attgcagcct tccatgatgg agacaaacgc
     9841 ggtcggccct tcggcgcgcg gttccggtag acggtcaaac ttctcgattt ccgggaagct
     9901 gatatctaca accgggctgc ggtcgccgcg cacggagttg atcatctccg gcagacggtg
     9961 cagcgtttgc ggcccaaaaa taatatcgac atagtgggcg cgctggcgaa tgtgctcgcc
    10021 ttcttgcgat gccacgcagc caccgacgcc gataatcagg tctggattct tctcttttaa
    10081 cagtttccag cgacccaact gatggaagac tttttcctga gccttctcgc ggattgagca
    10141 ggtgttcagc agcagcacat ccgcttcttc cgccacgtcg gtcagttgat agccgtgggt
    10201 ggcatccagc agatcggcca tcttcgatga atcgtactcg ttcatctgac agccccaggt
    10261 tttaatatgg agttttttgg tcatcgactt gctcttgcga aatagtagcc aggaatgcag
    10321 ggcgcatagt gtaatgcttt gctgccgttg tgaccagtat gagcgttatc agcccttagg
    10381 ggtaaaaatc ctgtaaactt aaagcatatt gctaacagga tgattgacca tgacaaatca
    10441 accaacggaa attgccattg tcggcggagg aatggtcggc ggcgcactgg cgctggggct
    10501 ggcacagcac ggattttcgg taacggtgat cgagcacgca gaacctgcac cgtttatcgc
    10561 tgacagccag ccggatgtgc ggatctcggc gatcagcgcc gcgtcggtat cattgcttaa
    10621 agggttaggg gtctgggatg cagcacaggc tatgcgttgc catccttacc gcagactgga
    10681 aacgtgggag tgggaaacgg cgcatgtggt atttgacgcc gctgagctta agctaccgct
    10741 gcttggctat atggtggaaa acactgtcct gcaacaggcg ttgtggcagg cgctggaagc
    10801 gcatccgaaa gtaacgttac gtgtgccagg ctcgctgatt gcgctgcatc gccatgatga
    10861 tcttcaggag ctggaactga agggcggtga aacgatccgc gcgaagctgg tgattggtgc
    10921 cgacggcgca aattcgcagg tgcggcagat ggcgggaatt ggcgttcatg catggcagta
    10981 tgcgcagtcg tgcatgttga ttagcgtcca gtgcgagaac gatcccggcg atagcacctg
    11041 gcagcaattt actccggacg gaccgcgtgc gtttctgccg ttgtttgata actgggcatc
    11101 gctggtgtgg tatgactctc cggcgcgtat tcgccagttg cagaatatga atatggtaca
    11161 gctccaggcg gaaatcgcga agcattttcc gtcgcgtctg ggttacgtta caccgcttgc
    11221 cgctggtgcg tttccgctga cgcgtcgcca tgcgttgcag tatgtgcagc cagggcttgc
    11281 gctggtgggc gatgccgcgc ataccatcca tccgctggcg gggcaggggg tgaatcttgg
    11341 ttatcgtgat gtcgatgccc tgattgatgt tctggtgaat gcccgcagct acggcgaagc
    11401 gtgggccagt tatcctgtcc tcaagcgcta ccagatgcgg cgcatggcgg ataacttcat
    11461 tatgcaaagc ggtatggatc tgttttatgc cggattcagt aataatctac cgccgctgcg
    11521 ttttgtgcgt aatcttggtt tgatggcggc ggagcgtgct ggcgtgttga aacgtcaggc
    11581 gctgaaatat gcgttagggt tgtagcctta caacattgtc ggatgcgtgc caaccatagg
    11641 tcggataaga cgcggcagcg tcgcatccga cattgaagga taagacgtgt caacatcgca
    11701 ttcgacattg aatgaacgca gaaaagcaaa aagctcgccg aagcgagctt ttttaatgtg
    11761 gctggggtac gaggattcga acctcggaat gccggaatca gaatcc
//
LOCUS       DAPGOF010000154        11803 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.155, whole genome shotgun sequence.
ACCESSION   DAPGOF010000154 DAPGOF010000000
VERSION     DAPGOF010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11803
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.155"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..449
                     /locus_tag="RHV13_003686"
     CDS             <1..449
                     /locus_tag="RHV13_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HDV1243175.1"
                     /translation="QILNAYDSSVYANDDIENLQQFMEMQRKMIIAQLIYYFGIAVST
                     SYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCALVVISGITGGLAYPLLKIW
                     MIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLARISRGIMPSLPFL"
     gene            complement(505..3360)
                     /gene="valS"
                     /locus_tag="RHV13_003687"
     CDS             complement(505..3360)
                     /gene="valS"
                     /locus_tag="RHV13_003687"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDV1243176.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLGADVVLRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEDQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(3360..3803)
                     /gene="holC"
                     /locus_tag="RHV13_003688"
     CDS             complement(3360..3803)
                     /gene="holC"
                     /locus_tag="RHV13_003688"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HDV1243177.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            complement(3937..5448)
                     /gene="pepA"
                     /locus_tag="RHV13_003689"
     CDS             complement(3937..5448)
                     /gene="pepA"
                     /locus_tag="RHV13_003689"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HDV1243178.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            5715..6815
                     /gene="lptF"
                     /locus_tag="RHV13_003690"
     CDS             5715..6815
                     /gene="lptF"
                     /locus_tag="RHV13_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HDV1243179.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6815..7897
                     /gene="lptG"
                     /locus_tag="RHV13_003691"
     CDS             6815..7897
                     /gene="lptG"
                     /locus_tag="RHV13_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HDV1243180.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(8016..9518)
                     /locus_tag="RHV13_003692"
     CDS             complement(8016..9518)
                     /locus_tag="RHV13_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HDV1243181.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAEHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDVKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPAAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(9648..10667)
                     /gene="ahr"
                     /locus_tag="RHV13_003693"
     CDS             complement(9648..10667)
                     /gene="ahr"
                     /locus_tag="RHV13_003693"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HDV1243182.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQCVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            10863..10947
                     /locus_tag="RHV13_003694"
     tRNA            10863..10947
                     /locus_tag="RHV13_003694"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10897..10899,aa:Leu,seq:caa)
     gene            11481..>11803
                     /locus_tag="RHV13_003695"
     CDS             11481..>11803
                     /locus_tag="RHV13_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243183.1"
                     /translation="MIKPFSVRYGHVDVREHVQLNDLNSDTRMALWNCLYLFLWTNNR
                     QTATATKCAQSVWIYYLNQPADNIPRYESGYKSDKTLLTAIRDYIYGEAWYLVYDLIE
                     FIIERT"
BASE COUNT         2660 a         3188 c         3024 g         2931 t
ORIGIN      
        1 atcaaatatt aaatgcgtat gatagttctg tgtatgcaaa tgatgatatt gagaatttac
       61 agcaatttat ggaaatgcaa cgtaaaatga taatcgcgca gttaatctat tattttggga
      121 ttgctgttag caccagttat ttaacggtgt cgttgcgaaa tcattttatg agcaacctgt
      181 cactgaatga tgggcgtatt cgttttcgct caactttaac gtaccacggt atgctttatc
      241 gcatgtgtgc gttggtggtg atatccggga ttacgggcgg tctggcttat ccactgctga
      301 aaatatggat gattgactgg caggcaaaaa atacgtattt gctgggcgat ttggatgacc
      361 ttcctttaat caataaagaa gaacaaccag ataaaggctt cttagccagg atttcacggg
      421 gaattatgcc ttctttacca tttctgtaag agataaaaaa ggccggagca tgctccggcc
      481 ttcgttttca ttactgtgtt ttgattacag cgcggcaata acagcctgct gttcaatcag
      541 cttcgctttc gcttctgcat agccttccag cttctcacgc tctttcgcga tgaccgcttc
      601 cggtgcgcgg gcgacaaagc cttcattcgc cagcttgttc tcgatacggc tgatttcgcc
      661 ttcgatcttc gccacttctt tcgccagacg cgccagctca tcttctttgt tgatgaggcc
      721 agccatcggg atcagcagct ctgcgccgtc gatgatctta gtaacggaaa ccggaccttt
      781 gtcatcggca ggcagcacgg tgatgctttc cagacgcgcc agggtttgca ggaagccacg
      841 gttttcattt acgcgacgtt ctgcatccgc gctgcaacca cgcagcagca gctccagcgg
      901 tttgcccggc gcgatgttca tttctgcacg gatgttacgt accgcaacga tcgcctgctt
      961 caaccactct gtgtcggcca gtgcggcttc atcaacctga gatgcatcgt actgcgggaa
     1021 cggctgcagc atgatggtgt cggcagtgat accgcaaagt actttcacac gctgccagat
     1081 ggtttcggtg atgaacggaa tgatcggatg cgcgaggcgc agcagacctt ccagcacggt
     1141 caccagcgta tggcgagtac cgcgcagttc ggcttcggtg ccaccgttca ttaccggctt
     1201 agtcagctcc agataccagt cacagaactg gttccaggtg aactcataca gaatgcctgc
     1261 ggcgatatcg aagcggaagc tgtccagcgc ttcgcggtac gctttgatgg tctggttgaa
     1321 ctccgccaga atccagcgat ccgccagcga cagcgtcatc tcgccgccgt tgaagccgca
     1381 atcctgatct tcggtgttca tcagcacaaa gcggctggcg ttccacagct tgttacagaa
     1441 gttacggtaa ccttccaggc gtttcatatc ccagttgata tcgcgaccgg tagaagccag
     1501 cgccgccagg gtgaagcgca gcgcgtcagt accgtgcggt tcaataccgt tcgggaactg
     1561 cttctcggtg cgcttacgga ttttgtccgc cagctgtggc tgcatcatat tgccggtgcg
     1621 tttttccagc agttctggca gcgaaatacc gtcaaccata tccagtgggt cgataacgtt
     1681 acccttggat ttggacatct tctggccttc gtcgtcacga atcagaccgg tcatgtaaac
     1741 agtgtggaac ggcacctgcg gtttgccatt ttcatctttg atgaagtgca tggtcatcat
     1801 gatcatgcgg gcaatccaga agaagatgat gtcgaaacca gataccatca cgctggttgg
     1861 gtggaactga cgcagggcgt cggtgttttc cggccagcca agggtagaga aggtccacag
     1921 cgcagaagag aaccaggtgt cgagaacgtc ttcgtcctga cgcaggacaa catcagcacc
     1981 gaggttattt tctttacgca cttcgtcttc gttgcggcca acataaacgt tacctgcttc
     2041 gtcgtaccat gcagggatac ggtgacccca ccacagctga cgagagatac accagtcctg
     2101 aatatcgcgc atccaggaga aatacatatt ttcgtactgc ttcggtacga actgaatgtc
     2161 gccattctca accgcttcaa ctgccggttt cgccaggaca tcggcacgca cgtaccactg
     2221 gtcggtcagc atcggttcga taactacgcc gccacggtcg ccgtaaggaa cggtcaggtc
     2281 gtgcggttta atttcttcca gcaggccgag cgcgtcaacc gcagcaacga ctgctttacg
     2341 tgcagcaaaa cgctccagtt tctggaattc tgccgggatc tcactggagt aaacgtcaga
     2401 ttcgttacct ttggtatcga acacctgggc gctttcacgg atatctccgt caaaggtcag
     2461 gatgttgatc atcggcaggg cgtgacgttt acccacttca tagtcgttaa aatcgtgcgc
     2521 cggagtgatt ttcacgcagc cggtgccttt ttccatgtcg gcgtgttcgt cgccaacgat
     2581 cggaatacga cggttaacca gcggcagaat gacatatttg ccaatcagat ctttgtaacg
     2641 cggatcttcc gggttaacgg ccacgccggt atcgcccagc agggtttccg gacgagtagt
     2701 cgcaaccacc agataatctt taccatctgc ggttttcgcg ccgtcagcca gcggatagcg
     2761 gatgtgccac atcgaacctt tcgattcgcg gttttccact tccaggtcag agatagcggt
     2821 gcgcagtttc ggatcccagt ttaccaggcg tttgccacgg taaatcaggt cttctttata
     2881 cagacgaacg aaaacttctt tcaccgcatt ggacaggcct tcgtccatgg tgaagcgttc
     2941 acgctcccag tccacggagt tgccgagacg gcgcatctga cgggtaatgg tgccgccgga
     3001 ttccgctttc cattcccaga ttttgtcgat gaaagcttcg cggccgtagt cgtgacgggt
     3061 tttaccttct tctgcggcaa tcttgcgctc aacgaccatc tgggtagcga tcccggcgtg
     3121 gtcagtaccg acctgccaca gggtgttttt gccctgcatg cgctgatagc ggatcatggt
     3181 atccatgatg gtttgctgga aggcgtgacc catatgcaaa ctgccggtga cgttcggcgg
     3241 cgggatcatg atgcagaaac tttcctggct ttcatcgcca ttaggcttaa agtagccctg
     3301 cttttcccag tgctcgtaaa gcggctgttc gatatcttgt gggttatatg tcttttccat
     3361 tatttccagg ttgccgtatt caggttgaaa ccagccacgc ggtaggcttt atagcgttcg
     3421 cgcgccagtt gtttcagaga atcttcataa ggaacgaagt ctaccacttc tgtgaaagcg
     3481 gtggcaaaat ctgcaaagct tgttcgcaga ctaatcaata tatcgcgcgg gctgctgctg
     3541 cgtttttgcg gccaggcgat ctccaccggc gcaccgccgc gcggtccttc tcccgctaaa
     3601 ttatgcggaa caaagctttc tgccggacgc gcccacaggg cttcatccag ccggtaagcc
     3661 tgcttttcat cttcacaggc gatgagtacg cgcttaccac tgcgccaacg ttctgcggca
     3721 atttcacaca ccagttgctc aacggcgctt aagccatcga cggtggtgtc attgtccaga
     3781 aggtagaacg tcgcgttttt cataaatggg gcttcttgtg gtggatttaa atattgctgc
     3841 attcctcacc ctaaaagatg agcaatgaat ttctattgcg ctcgatcggt tccctctccc
     3901 ttagggagag ggttagggtg agggtaaaac gcaaacttac tcttcgccgt taaacccggc
     3961 gcggtttaac aggaactgtg ccagcaacgc taccggacga ccggttgcgc ctttcgcttt
     4021 accagaacgc caggcggtac cggcgatatc caggtgcgcc cagttgtact tacgggtaaa
     4081 gcgtgacagg aagcaacctg cggtaatcgc cccaccagga cgaccgccaa tgttcgccat
     4141 atcggcaaaa ttggactcca gttgttcctg atactcgtca cccagcggta agcgccatgc
     4201 gcggtcaccg gattgttcag acgcggcaat cagttcatgg gccagcggat tgtggttcgc
     4261 catcagacca gtaatgtgat gacccagcgc gatcacgcag gcaccggtca gcgtcgccac
     4321 gtcaatcacc gcttccggct caaaacgctc aacgtaagtt aacacgtcgc acagtaccag
     4381 gcggccttca gcgtcggtgt tgagcacttc aacggtttga ccagacatgg tggttaacac
     4441 atcgcccgga cgataggcgc gaccgcctgg catgttttca caacctgcca gcacgccgat
     4501 aacattaatc ggcagttgta gctccgcgac catacgcatc acgccgtaaa ccgccgccgc
     4561 gccgcacata tcgtacttca tctcatccat gccttctgaa ggcttgatcg agataccgcc
     4621 ggagtcgaag gttaaacctt tacccaccag cacgattggg cgtgcatctt ccgacgcgtt
     4681 gcctttgtac tcaatcaccg acatcagcga ttcgttttgc gaaccctgac cgaccgccag
     4741 ataagagtgc atccccagct ctttcatctg ctgttcgccg ataacgcggg taatgacatt
     4801 cttgctgtag ctgtcagcca gctggcgcgc ttgtgaagcg aggtaagcgg cgttacagat
     4861 attcggcggc atattgccga gatcttttgc tgctttaatc ccggcggcaa tcgccagacc
     4921 gtgctggatc gcgcgctcac cgctggtcag ttcacggcgg gtcggcacgt tgaacaccat
     4981 cttacgcagc ggacgacgcg gttcgctctt gttcgttttc agctgatcga aactgtagag
     5041 cgtctctttt gccgtctcga cagcctgacg cactttccag tagttgttac ggcctttaac
     5101 gtgcagttca gtcagaaagc agaccgcttc cattgagcca gtatcattca gcgtattaat
     5161 ggttttctga ataacctgct tgtactgacg ctcatccagc tcacgttctt tgccgcaacc
     5221 aataaggaga attcgctcgg aaagtacatt cggaacatgg tgcagcaaca atgtctgccc
     5281 cggttttcct tccagttcgc cccgacgtag cagggcgctg atgtacccat cgctgatttt
     5341 atcgagctgt tctgcaatcg gagaaaggcg acgtggttcg aagacgccca cgacgatgca
     5401 ggcactccgc tgtttctccg ggctaccgct ttttacacta aactccatgc actacgctcc
     5461 tgaatcttaa agacaacggc ggtggctaca gatagaattg caagctttcg taactcatgt
     5521 ccgctgttgc gatgacttcg tgttaatctt aacgttatta cggcattggc acgtcagaac
     5581 aaaatctgag aggtgaatcc gttgagtata attatcttag cgacgatttc gacgactcaa
     5641 gagaataaat gacgtttaag ccatgaaaca agctaaaatc ctgcaaaaga cgagttttta
     5701 cgggcgtatt taaagtgata atcataagat atctggtgcg ggagacgctc aaaagccagc
     5761 tggcgatact cttcatcttg cttttgatct tcttctgtca aaagttagtg aggatcctcg
     5821 gcgcagcggt tgacggcgat attccggcga atctggtgct ctcccttctc gggttgggcg
     5881 tgccggaaat ggcgcagctt atcctgccat taagcctgtt cctcgggctg ctgatgacgc
     5941 tgggcaaact gtataccgaa agtgaaatta cggtaatgca tgcctgcggc ctgagcaaag
     6001 cggttctggt gaaagcggca atgatccttg cggtattcac ggcaatcgtc gcggcggtta
     6061 acgtgatgtg ggcgggaccg tggtcatcgc gtcatcagga tgaagtgtta gcagaagcga
     6121 aagcgaaccc tggcatggcg gcgctggcgc aagggcaatt ccagcaagcg actaatggca
     6181 gttcggtgct gttcatcgaa agcgttgacg gcagcgattt caaagatgtg ttcctcgcgc
     6241 aaattcgacc aaaaggtaat gcacgtcctt ctgtggtggt ggccgattcc ggacatttaa
     6301 cccagctgcg cgacggctcc caggtcgtca ctctcaacca gggaacgcgc ttcgaaggta
     6361 ctgcactgtt acgtgatttc cgcattacgg atttccagga ttatcaggcg atcattggtc
     6421 accaggcggt ggcgctcgac ccgaacgata ccgaccagat ggacatgcgc acattgtgga
     6481 acactgacac cgatcgtgct cgcgcagaac tgaactggcg tatcacgttg gtattcaccg
     6541 tgtttatgat ggcacttatg gtcgtaccgc tgagcgtggt taacccacgt cagggacgcg
     6601 tactgtcgat gctgccagcc atgctgctgt atctgttgtt cttcctgatc cagacctccc
     6661 tgaaatcgaa cggcggtaaa ggtaagctgg acccgacgct gtggatgtgg accgttaacc
     6721 tgatttatct ggctttggcg attgttctca acctttggga caccgtgccg gtccgccgcc
     6781 tgcgcgccag tttttcgcgt aaaggagcgg tgtgatgcaa ccttttggcg tacttgaccg
     6841 ctatatcggt aaaactattt tcaccaccat catgatgacg ctgttcatgc tggtgtcgct
     6901 gtcgggcatt atcaagtttg tcgatcagct gaaaaaagcc gggcagggga gttacgacgc
     6961 gttaggcgca ggaatgtata ccttgctgag cgtgccgaaa gatgtgcaaa tcttcttccc
     7021 gatggcggct ctgcttgggg cgttgcttgg tcttgggatg ctggcgcagc gcagcgaact
     7081 ggtggtgatg caggcttctg gttttacccg tatgcaggtg gcgctgtcgg tgatgaaaac
     7141 cgccattccg ctggtcttac tgacgatggc gattggtgaa tgggtcgcgc cgcagggcga
     7201 acagatggcg cgtaactacc gtgcgcaggc gatgtacggc ggctcgttgc tctctaccca
     7261 gcaaggctta tgggcgaaag atggcaacaa cttcgtctac attgagcggg ttaaaggtga
     7321 cgaagagtta ggtggcatca gcatttatgc ctttaacgag aatcgtcgtc tgcaatccgt
     7381 acgctatgcc gctactgcga agtttgaccc ggaacataaa gtctggcgtc tgtcgcaggt
     7441 tgatgaatct gatctgacca atccgaaaca gattaccggt tcgcagacgg tgagcggcac
     7501 ctggaaaacc aacctcacgc cagacaaact gggcgtggtg gcgctggacc cggatgcact
     7561 ctcaatcagc ggtttgcaca actacgtgaa gtatctgaag tcgagcggtc aggacgctgg
     7621 acgttatcag ctcaacatgt ggagcaaaat cttccagccg ctatccgtgg cggtgatgat
     7681 gctgatggcg ctgtcgttca tctttggccc actgcgtagc gtaccgatgg gcgtgcgtgt
     7741 ggtcaccggt atcagcttcg gttttgtctt ctacgtactg gaccagatct tcggcccgtt
     7801 gacgttggtt tatggcatcc cgccgatcat cggcgcactg ttgccaagcg ccagcttctt
     7861 cttaatcagc ctgtggctgt taatgagaaa atcgtaacca agcaagggag cgaaagctcc
     7921 ctttcacatt gttgataaag tcaaatacga gttcgatcgg tcccctcgcc cctttgggga
     7981 gagggttagg gtgaggggca cagaccgcac ctgacttacc ttcttctccc ccccagcaaa
     8041 ctccccaaca tcccgcgtac aatctgattc gtcacctggc gagcggcgct tttggccatt
     8101 gtttgcacca caccatcttt cttcccgccg cgtggtccgg tagtgccaaa caaaatatcc
     8161 ttcaatccac caagaatacc gtcatccacc gccacctctt tccctttcgc ggcaggatta
     8221 ttttgctgct cggtactggc ctgaaagcct ttttgcaaca tctcataggc ggattctcgg
     8281 tccacctcat cctcatattt gccatacacc ggggagtgat taatcaggcc attacgctca
     8341 tcttccgtca ccggccccat tcgcgaacaa ggcgcgatca ccatcgcccg ctccaccact
     8401 gaaggacttc ctttcacatc aagaaacgag attaacgcct cgccggtgcc cagttcctga
     8461 atcgcctttt cggtatcaaa cgccggattg gcccgcatgg tttgcgccgc agcttttacc
     8521 gctttctgat ctttgggcgt aaaagcacgc aaagcgtgtt gaacgcgatt accgagctgc
     8581 ccgagcacgt tatccggaat atcagacggg ttttgcgaaa cgaaccagac gcccacgcct
     8641 tttgagcgaa taagccttat cacctgctca atcttatcca gcagtacctg cggtgcgtca
     8701 ttaaacagca gatgtgcttc gtcgaagaaa aacaccagtt tcggcttctc cagatcgcct
     8761 gcttccggca attgttcata caactccgag agcatccaca acaggctggc ggcatatagt
     8821 ttcggcatct gataaagctt ttcggcgctg aggatattga taacgccttt accgttggca
     8881 tcggtgcgca tccagtcttt gatatccagc atcggctcgc caaagaaatg ctccgcacct
     8941 tgttgctcca gcgacagtaa tccgcgctgg atggcaccaa ccgatgcgct actgatattt
     9001 ccgtactgat tctggaaaga tttggcgtta tcgccgatgt actgggtaat cgcccgcaaa
     9061 tctttaaagt cgagcagtaa cagcccctga tcgtcagcaa tgcggaagat gatattcagc
     9121 acgccagact gcacatcgtt gagattcaac agccgcgcca gcaacagcgg ccccaggtct
     9181 gaaaccgtcg cccgcaccgg atggcctttc tcgccaaaga tatcccacac caccaccgga
     9241 ttggcatgcg gttgccagtc attgacgccg atatttttaa gccttgtgag cagtttttcc
     9301 gacgccgttc ctgcctgcgc gataccggtc agatcgcctt tcacatcagc cataaacacc
     9361 ggcacgccga tttccgacaa tgactctgcc agtttttgca gcgtaacggt tttacccgtc
     9421 cccgttgcgc cagtaatcag cccgtgacgg ttagccattc ccggcagtaa aaacagttct
     9481 gtgtccggcg tgcgggcaat taacaggggt tcgctcatga gatttcctcc agtttcctgc
     9541 ctggagtata ggcaacggct gtgcactctt caccgctaaa ttcagcagat tgcgagacgc
     9601 cttccaaaga gatttaagtg ccgggcgaca ggcacccggc aaagggatca gaagtcggct
     9661 ttcaggacca cgcggtagcg cgctttacca tcgcgtacat gctggatggc gtcgttaatt
     9721 ttcgacatcg ggaacagttc ggtggtcggc gcaaccttgc tgcgggcggc aaaacgcatc
     9781 agcttacgca gctcataagg cgtgccggta gcagaaccag agacgctgcg atcgcccgca
     9841 attaacgtaa aggccggaac agacagcggc gtgagaaccg caccgaccgt atggaaatta
     9901 ccgccatagg tcagcgcctc aaaataaggc tgccagtcga ggctgacgtt gacggtgttg
     9961 ataatgagat caaactgccc cgccagtgct ttcagtgcct gcggatcgcg gctattcacc
    10021 actttatcgg cacccatcgc cagtacttcc tgctctttcg ccggattaga actaaaggcc
    10081 gtcacctcgc atcccattgc gtgcagaagt tttatagcga tatgccccag cccgccaata
    10141 ccaattaccc caacgcggct ggtagcagtg atatggtgca tcaacagtgg tttaaagacc
    10201 gtgataccgc cgcacaacag cggcccggcg gactcgatat caatattttc tggcagtgga
    10261 atcacccatt gccagtccgc acgcaacttc tcggcaaagc caccgcgatt cataatcgtc
    10321 ggcaccgcac cttgctcgca gttgatctga ttaccgctaa tacaggcgtc gcagtgacca
    10381 cagctacgcg ccgtccagcc aatcccgaca cactgaccga cctgcaaacc tttatcctgc
    10441 gcggcgctcc cgagtgccac cacgcgccca atcacctcat gcccggcaac cagcggatat
    10501 tgtgaaaatc cccattcgtt atcgatcatc gacagatcgg aatgacaaat cccgcagtaa
    10561 tccacctgca cttcaacatc ttgtggcttc agctcaccgg gatcgtactc ataaacttcc
    10621 agttcgccgc ccgcttcttt tgcggcatag ctttttatca tcgacatttt tgggtccttc
    10681 tctggtgttg tttgggaagt gtagagcatg gcagggcggg gtgcctggag tgtgacaaag
    10741 gttacatatc gctgagtgta atgctgaaaa tttcagcact tagcgaggtg ggtgcaagct
    10801 ggcgcttgca tggtggcgtg cgacaggtat aatccacaac gttttccgca tacctcttca
    10861 gtgccgaagt ggcgaaatcg gtagacgcag ttgattcaaa atcaaccgta gaaatacgtg
    10921 ccggttcgag tccggccttc ggcaccaagt tactaaattc cttacaatac agttagttag
    10981 acgattaaat ctgtctcttt tgcctgtaag aaatgctctg ttatgtccct tgaaaaagcc
    11041 gctgtggttg atagtggtaa ttgggatgtt aactcatagc ggcgtgtcgt tccaagcgtc
    11101 aaacacgtac tgactcaact tgaaagctat gacgtagcca ccgtctctaa acaatcccaa
    11161 aacttttagt gcagcgcatt ttagcccgat gcccccattc tgtcgccgtc gcgagacgcc
    11221 gcaggggata aacaggttta tggtggtcac ggtacttgca gaccatatgc aagctctgcc
    11281 agcggttcgg cctcgctccc cttgggtaca tggtcatcct gataagcctc actaactgac
    11341 ataaccctga tgaagtcgca agaggctccg cttgttgtct taccaccaac ggggctttat
    11401 ggattctatt gcttaatata tgattgtatc aaacttagac tgcataattt aaacgaacat
    11461 aacaatcagg caggggaaga atgataaagc cattttctgt gaggtatggt catgttgacg
    11521 ttagagagca tgtccagctg aatgatctta attctgatac acgcatggca ttgtggaatt
    11581 gcttatatct tttcctttgg actaacaatc gtcaaactgc tacagcaaca aaatgtgcgc
    11641 agtctgtgtg gatatattat ctaaatcaac ctgcggataa tattcctcgt tatgaaagtg
    11701 gatacaaaag tgataagacg ttgctgactg ctatccgaga ttatatatat ggggaagcat
    11761 ggtatttggt atatgattta attgagttca tcattgagag aac
//
LOCUS       DAPGOF010000155        11795 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.156, whole genome shotgun sequence.
ACCESSION   DAPGOF010000155 DAPGOF010000000
VERSION     DAPGOF010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.156"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            47..1198
                     /gene="yiaY"
                     /locus_tag="RHV13_003696"
     CDS             47..1198
                     /gene="yiaY"
                     /locus_tag="RHV13_003696"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HDV1243184.1"
                     /translation="MAASTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            1328..2623
                     /locus_tag="RHV13_003697"
     CDS             1328..2623
                     /locus_tag="RHV13_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDV1243185.1"
                     /translation="MAIKSPPGLIPLSHLSGEELLAHLRFNRVTDEKGRYLPFDELQY
                     RIKKGENVDVAWTLTRLARNAAIQRINYCNEAGEQAGFNITPVIAEACELVDKRATAL
                     ALKDQTERLRGAGAELSQLRLEEPITSSQLEGANTTTLVARKMLETGRSPRTEDEHMI
                     AGNARLMAEIPHLLAEPLTPALIRQLHAIGMGGINDAKYRPGEFRETDDVVIADYDGN
                     IVHQPPAAALLPERLEKVCQWLNSHEGYIHPLVRACILHFMLAHEHPFRDGNGRTSRA
                     LFYWYMLKSGYDVFKYISISRLLHAAPVKYAASYQYTESDGMDLTYFLEYQAGVIKRA
                     LQNWQQHIDEITQRSAKLDSVLFSSGVLKRLNPRQVTLLNVMLANPGKEHTVAEISAS
                     LGVSDNTARADLRTIVKEGFAQEKKINDQQAVYFAHYPL"
     gene            2731..4269
                     /gene="aldB"
                     /locus_tag="RHV13_003698"
     CDS             2731..4269
                     /gene="aldB"
                     /locus_tag="RHV13_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HDV1243186.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(4488..4643)
                     /locus_tag="RHV13_003699"
     CDS             complement(4488..4643)
                     /locus_tag="RHV13_003699"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243187.1"
                     /translation="MAKFMIKNRKTSTMQIKRIKPSIIYRLLLIVLGIPMVIYGLVCP
                     LTIETRD"
     gene            4814..5137
                     /locus_tag="RHV13_003700"
     CDS             4814..5137
                     /locus_tag="RHV13_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HDV1243188.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQSHDSSVMQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            5143..6279
                     /locus_tag="RHV13_003701"
     CDS             5143..6279
                     /locus_tag="RHV13_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDV1243189.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMVANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(6276..7250)
                     /locus_tag="RHV13_003702"
     CDS             complement(6276..7250)
                     /locus_tag="RHV13_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV1243190.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTPFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
     gene            7374..8114
                     /locus_tag="RHV13_003703"
     CDS             7374..8114
                     /locus_tag="RHV13_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HDV1243191.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDTFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEVTDSPMIDRNESFTFVTGVSWRF"
     gene            complement(8235..10214)
                     /locus_tag="RHV13_003704"
     CDS             complement(8235..10214)
                     /locus_tag="RHV13_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003581012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HDV1243192.1"
                     /translation="MNISEVDLHKLTVSDPFLGQYQQLVRDVVIPYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQEGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LIASAQCEDGYLNTYFTVKAPEERWSNLAECHELYCAGHLIEAGVAFFQATGKRRLLG
                     VVCRLADHIDSVFGPDESKLHGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGA
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQAHLPLAQQQTAIGHAVRFVYLMTG
                     VAHLARLSHDDSKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFTSDYDLPNDTVYAE
                     SCASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKS
                     LKFNHIYDHVKPIRQRWFGCACCPPNIARVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRVSGNYPWQEQVTIAVESPQPVRHTLALRLPDWCTQPQIILNGEEVEQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEQADN
                     GESLHNLWLPTDAPFTTFEGKGLFSHKILIQAPGYRYEQSNPEQQPLWHYDSAPAKRQ
                     PQTLTFIPWFSWANRGEGEMRIWVNEEKHRHPEVG"
     gene            complement(10225..11625)
                     /locus_tag="RHV13_003705"
     CDS             complement(10225..11625)
                     /locus_tag="RHV13_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1243193.1"
                     /translation="MTSTPITTADIAKGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIAWAFGTYLLLSVGYTAINVPYCALINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVDLFGQGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNLLLAALAVLMWFLPSGPA
                     YQTLWLAVILGNGVILGFTLPLHFSLMAFADDYGEWKTRVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTASSLGGIAAMETLLPALFHLLLAGAIRFCKLNNP
                     MMSRIATDLRQRHVQP"
BASE COUNT         2915 a         2893 c         3095 g         2892 t
ORIGIN      
        1 tcctcagtaa gctgcccacc cttttttaca ctttcaggag tgtgttatgg cagcttcaac
       61 cttttttatt ccttctgtga atgtcatcgg cgctgattca ttgactgatg caatgaatat
      121 gatggcagat tatggattta cccgtacctt aattgtcact gacaatatgt taacgaaatt
      181 aggtatggcg ggtgatgtgc aaaaagcact ggaagaacgc aatattttta gcgttattta
      241 tgatggcacc caacctaacc caaccacgga aaacgtcgcc gcaggtttga aattacttaa
      301 agaaaataat tgcgatagcg tgatttcctt aggcggtggt tctccgcatg actgtgcaaa
      361 aggtattgcg ctggtggcag ccaatggtgg tgatatccgt gattatgaag gcgttgaccg
      421 ctctgcaaaa ccgcagctgc cgatgatcgc catcaatacc actgcgggta cagcatcaga
      481 aatgactcgt ttctgcatca tcaccgacga agcgcgtcac atcaaaatgg cgattgttga
      541 taagcacgtg actccgctgc tttctgtcaa tgactcatct ctgatgatcg gtatgccgaa
      601 gtcactgacc gccgccactg gtatggacgc cttaacacac gctatcgaag cgtatgtttc
      661 tatcgctgca acgccgatca ctgacgcttg cgcactgaaa gcggtgacca tgattgccga
      721 aaacctgcca ttagccgttg aagatggcag caatgctaaa gcgcgtgaag caatggctta
      781 tgcccagttc ctcgctggta tggcgttcaa caacgcatct cttggttatg ttcatgcgat
      841 ggcacaccag ctgggcggtt tctacaacct gccacacggt gtatgtaatg ctgttctgct
      901 gccgcatgtt caggtattca acagcaaagt cgccgctgca cgtctgcgtg actgtgccgc
      961 tgcaatgggc gtgaacgtga caggtaaaaa cgacgcggaa ggtgctgaag cctgcattaa
     1021 cgccatccgt gaactggcga agaaagtgga tatcccggca ggcctacgcg acctgaacgt
     1081 gaaagaagaa gatttcgcgg ttctggcgac taatgccctg aaagatgcct gtggctttac
     1141 taacccgatc caggcaactc acgaagaaat tgtggcgatt tatcgcgccg caatgtaatc
     1201 atcattccaa taacggctgg caaattgtca gccgcttttt caactatctc aaaacttgct
     1261 ttttttgaga tagtttctct ttcctgctat acactatctc aatattgcgc ttttttgaga
     1321 taagcacatg gctatcaaat cacctcccgg acttattcct ttatcacact tatcgggtga
     1381 ggaactcctg gcccacctgc gctttaaccg ggttaccgat gaaaaaggac gttatctgcc
     1441 ttttgatgag ttgcagtacc gcatcaaaaa aggagaaaac gtcgatgtgg cgtggacgct
     1501 tacccgtctg gcgcgcaacg cggcgatcca acgaattaat tattgcaatg aagcgggtga
     1561 acaggccgga ttcaacatta ccccggtgat tgcagaagcg tgcgagcttg tcgacaaacg
     1621 tgctaccgcg ctggcgctga aagatcagac tgaacggtta cgcggggccg gagccgagtt
     1681 aagccagtta cgcctggaag agccgatcac cagctcccag ctggaaggtg cgaatactac
     1741 aacgctggtg gcgagaaaaa tgctggaaac aggacggtcg ccacgcactg aagacgagca
     1801 tatgattgcg ggaaatgccc ggctgatggc ggaaattccg cacttactcg cagaaccgct
     1861 gacacctgcg cttattcgcc agttacatgc cattggtatg ggtggaatca atgacgcgaa
     1921 atatcgtccg ggagaatttc gtgaaacaga tgatgttgtg atcgccgact atgatggcaa
     1981 tatagtgcat caaccgcccg ctgcggcatt gttaccggaa cggctggaaa aagtttgcca
     2041 gtggttaaac agccatgaag ggtatattca tccgttggtc cgtgcctgca ttctgcattt
     2101 tatgctggcc catgaacatc cgtttcggga tggcaacggg cgcaccagtc gggcgctgtt
     2161 ttactggtat atgctgaaat cgggctacga tgtctttaaa tatatttcca tcagccgttt
     2221 gcttcatgct gcaccggtca aatatgccgc cagctaccag tacactgaat cagatggtat
     2281 ggatctgact tatttccttg aatatcaggc gggtgtcatt aaacgtgctc tgcaaaactg
     2341 gcagcagcat atcgatgaaa ttacacaacg aagcgcaaaa ctcgatagtg tgctgtttag
     2401 ctctggtgtg cttaaacgcc tgaatccgcg ccaggtaacg ctcctgaatg tgatgctcgc
     2461 caatccgggg aaagagcaca cagttgctga aattagcgcg agcctgggcg tctctgataa
     2521 tacggctcga gctgatttac ggacaattgt taaagaaggg tttgcgcaag agaagaaaat
     2581 taatgaccag caagccgtgt acttcgcgca ttatccgctg taacccttgc ccgtaaattc
     2641 gtgatagctg tcgtaaagct gttaccgact ggcgaagatt tcgccagtca cgtctaccct
     2701 tgttatacct cacaccgcaa ggagacgatc atgaccaata atcccccttc agcacagatt
     2761 aagcccggcg agtatggttt ccccatcaag ttaaaagccc gctacgacaa ctttattggc
     2821 ggcgaatggg tagcccctgc cgatggtgag tattaccaga acctgacgcc ggtgaccggg
     2881 cagctgctgt gcgaagtggc gtcttcgggc aaacgagaca tcgatctggc gctggatgct
     2941 gcgcacaaag tgaaagataa atgggcgcac acctcggtgc aggatcgcgc ggcgattctg
     3001 tttaagattg ccgatcgaat ggaacaaaac ctcgagctgt tagcgacagc tgaaacctgg
     3061 gataacggca aacccattcg cgaaaccagt gctgcggatg taccgctggc gattgaccat
     3121 ttccgctatt tcgcctcgtg tattcgggcg caggaaggtg ggatcagtga agttgatagc
     3181 gaaaccgtgg cctatcattt ccatgaaccg ttaggcgtgg tggggcagat tatcccgtgg
     3241 aacttcccgc tgctgatggc gagctggaaa atggctcccg cgctggcggc gggcaactgt
     3301 gtggtgctga aacccgcacg tcttaccccg ctttctgtac tgctgctaat ggaaattgtc
     3361 ggtgatttac tgccgccggg cgtggtaaac gtggtcaatg gcgcaggtgg ggaaattggc
     3421 gaatatctgg cgacctcgaa acgcatcgcc aaagtggcgt ttaccggctc aacggaagtg
     3481 ggccaacaaa ttatgcaata cgcaacgcaa aacattattc cggtgacgct ggagctgggc
     3541 ggtaagtcgc caaatatctt ctttgctgat gtgatggatg aagaagatgc ctttttcgat
     3601 aaagcgctgg aaggctttgc actgtttgcc tttaaccagg gcgaagtttg cacctgtccg
     3661 agtcgtgcct tagtgcagga gtcaatctac gaacgcttta tggaacgcgc catccgccgt
     3721 gtcgaaagca ttcgcagcgg taacccgctc gacagcgtga cgcaaatggg cgcgcaggtt
     3781 tctcacgggc aactggaaac catcctcaac tacattgata tcggtaaaaa agagggcgct
     3841 gacgtgctca ccggcgggcg gcgcaagctg ctggaaggcg aactgaaaga cggctactac
     3901 cttgaaccga cgattctgtt tggtcagaac aatatgcgcg tgttccagga ggagattttt
     3961 ggcccggtgc tggcggtaac caccttcaaa acgatggaag aagctctgga gctggcgaac
     4021 gatacgcaat atggcctggg cgcgggcgtc tggagccgca acggtaatct ggcctataag
     4081 atggggcgcg gcatacaggc tgggcgcgtg tggaccaact gttatcatgc ttacccggca
     4141 catgcggcgt ttggtggcta caaacaatca ggtatcggtc gcgaaaccca caagatgatg
     4201 ctggagcatt accagcaaac caagtgcctg ctagtgagct actcagataa accgttgggg
     4261 ctgttctgat gtaagaagct ggtcgcatcg ggtattaatt gcctgatgcg acgcttacgc
     4321 gtcttatcat gcctacgggg acctgaccgt aggccggata aggcgtacac gccgcatccg
     4381 gcataaacaa agcgcacgtt gttacaattt acgttttaaa cctacgtgtg caataaccca
     4441 gactacgcca acaaaagtgc gtttctgggg cgtgttttgc caggagttta atctcgcgtt
     4501 tcgatggtga gcgggcaaac cagaccgtaa atcaccattg gtattccgag gacaatcagc
     4561 aataaacggt agattattga aggtttaata cgcttaatct gcattgtgga tgttttacgg
     4621 tttttaatca taaatttggc caatagttcc attatttcaa tatgataaac ctcgacctgt
     4681 tgaaaagcca ggcagcacac ttcaggaagt atataaaaaa caatatgtcg ttatataaat
     4741 aaggaaattg cactgcatca tggcatttca taatatgcgt taattgttct tttttatatt
     4801 ctctgaggga aatatgttcc tggactattt tgcactggga gtgcttattt ttgtatttct
     4861 ggtgatattc tatgggatta ttattttaca tgatattccc tacctgattg ctaaaaaacg
     4921 taatcatcct catgccgacg ccattcatgt tgctggttgg gtgagtcttt ttacgttaca
     4981 tgttatctgg ccgtttttgt ggatttgggc cacgctttac cgcccggagc ggggatgggg
     5041 aatgcaaagc catgattcat ccgttatgca actgcaacag cgcattgccg ggctggagaa
     5101 acagctcgcc gacatcaaat cctcttctgc cgagtaatac ttatggattt actgattatt
     5161 ttgacctatg tggcttttgc atgggcaatg tttaagatct tcaaaattcc cgtaaataaa
     5221 tggaccattc ccacagcggc cctgggtgga atatttattg tcagcggtct aattctgtta
     5281 atgaactata accatccgta tacctttaaa gcgcaaaaag cggttatttc tattcctgtt
     5341 gtcccacagg tgactggtgt ggtgattgaa gtgacggata agaaaaatac gctgattaaa
     5401 aaaggtgaag tgctatttcg actggacccg acgcgttatc aggcgcgggt ggatcggctg
     5461 atggcggata tcgttaccgc agaacataaa cagcgggcgt tgggcgcaga gttagatgag
     5521 atggtggcga atactcagca ggcaaaggcc acgcgggata aattcgctaa agagtatcag
     5581 cgttacgcac gcggcagcca ggcgaaagta aacccgtttt cagaacgcga tattgatgtg
     5641 gcgcggcaaa attatctggc gcaggaagcc tccgtaaagt catcggcggc ggaacaaaaa
     5701 cagatccaga gccagctgga tagcctggtg ttgggtgaac attctcaaat cgccagcctg
     5761 aaagcgcagc tcgcggaagc aaaatataac cttgagcaga cgatagtgcg tgcgccgagc
     5821 gatggttatg tgactcaagt gctgattcgt ccgggtacct atgccgcgtc gctgccgcta
     5881 cgtccggtga tggtgtttat acccgatcag aaacgacaaa tcgtggcgca gttccgtcag
     5941 aactccttgc tgcgcctggc tcctggcgac gatgcggaag tggtgtttaa tgctctgcca
     6001 ggtaaggtat tcagcggtaa gctggcagcc attagtccag ccgttcccgg cggagcttat
     6061 cagtcgaccg gcaccttaca gacgttaaac acagcgccgg gttcagatgg cgttatcgcg
     6121 accattgaac tggatgagca cactgatttg agcgctttac cagacggtat ttacgcccag
     6181 gtggcggtct actctgatca tttcagccat gtctcggtga tgcgcaaagt gctgttacgc
     6241 atgaccagtt gggtgcatta cctttatctc gatcattaat cttcgctttc cgccgcttgt
     6301 tcaggccgca gttgctgcga ctgagcaagc tggtggcgaa tttcacgcag catttcatcc
     6361 agcacatact ggacccgcca gggctggtat tcccgtttac ggaagatagc caccagatcc
     6421 agccaccatt catacgggtg ctgaaagcag ggcaccagcg tgccgtcttg caggtcgctc
     6481 tgcacacttt tgtctggcgc aaacacaatc cccaggtgat ttcgtgccag ctccagcgcc
     6541 gattgcgtgt tatcgcaaac gtaatttccc ttcacgcgat aatcccgcac ctctttactc
     6601 ccggcgacgt tgaagcgcca gatattcgcg tcgtcgatca tcatcgagtc gatcaaaata
     6661 caggagtgat gttcgagttc gtcaggacgg ctaatgggat gtttctcaag atagcgctgg
     6721 ctggcataag cggttacggc atattgggta ataaaactgg caaccagcga ttcatctttt
     6781 ggtggggcat agctgattaa aacatcacaa tcatcaggaa attcgacgcc ttcataaaag
     6841 gcattacgct caagattgca ggtttttagc gataaggtga tatcaccgat atctttaatt
     6901 ttatcgatta catgtttgga taaataggta attatgccgg ttggggcgta gatggtgacc
     6961 cgaccacgtt tctcatgctt ataatctgca ataaaattat taagctgctc gtttctgtcc
     7021 agcatgtcgt tgatgtacgg taatagcgcg gtgccaaaag gtgtgagcat cagttgtcgg
     7081 gtggttcggt caaaaacttt taaacccact tttgattcaa aatcagcaag atatttgctg
     7141 acgttggcct gtgcgatgcc aagtacagtc gcggcatggc tgatattttc actggcagcg
     7201 attaccgaga taatttttaa ctcccggtac ttaagttgta attttgtcat cgcaacagcc
     7261 caattatata taattttata tattactata taaatatgaa tcttgcaccg gagaaattgt
     7321 aagcattact atgccgactc ttttttatct tattttaaaa tggaataccg gacatgttaa
     7381 ttaaccgcaa tattgtggcg ttatttgcgt tgccttttat ggcaagcgca actgcttctg
     7441 aattatccat tggtgccggt gcggcttata atgaatcgcc ttatcgcggt tataatgaaa
     7501 atacaaaggc aattccgctg attagctatg aaggtgatac tttttatgtt cgtcagacca
     7561 cgttaggttt tattctgtcg caaagtgaaa aaaatgaact tagcctgacc gcatcctgga
     7621 tgccgctgga atttgaccct gccgataatg acgattatgc catgcaacag cttgataagc
     7681 gtgatagtac ggctatggcg ggggttgcct ggtatcacca cgagcgttgg ggaaccgtga
     7741 aagcctctgc agctgcggac gttctggata acagcaacgg ctgggtgggg gagctatcgg
     7801 tattccacaa aatgcagata ggtcgtctgt cgctgacacc tgcgctgggc gttctctatt
     7861 atgacgagaa tttcagtgac tattactatg gcatttcaga gagtgagtca cgtcgtagcg
     7921 gtctggcaag ttattccgcg caggatgcct gggtgcccta tgtcagcctg acggcaaaat
     7981 acccgatagg agagcacgtc gtattgatgg cgagcgcagg atacagcgag cttccggaag
     8041 aggttaccga cagcccgatg attgatcgta atgagagttt tacctttgtc accggcgtga
     8101 gctggcgttt ttaattcacc ggtggatgtc ggtgcggctg gcctacgctc tcgcaccgac
     8161 tcgtatgcct gataagacgt gttaagcgtc gcatcaggca atgtgctccc tacgttggat
     8221 gcggcgcgag cgccttatcc aacctccgga tggcgatgct tttcctcatt cacccagatc
     8281 cgcatttcgc cttcgccccg gttagcccag ctaaaccacg ggataaacgt cagagtttgc
     8341 ggctggcgtt tggctggcgc gctgtcgtaa tgccacagcg gttgctgctc tggattgctc
     8401 tgttcatacc ggtaacccgg tgcctggatt aagatcttat ggctaaacaa tcccttgcct
     8461 tcaaatgtcg taaatggcgc atcggtgggc agccacagat tatgcagtga ctcgccgttg
     8521 tcggcctgtt ccaggcaata caccagcggg ccgcgctgaa tcgccacttt tccggcgacg
     8581 tgacgcacca gcgggttacc gtaaacgcgg cgtaccggca tcggcaaagt cagattcagc
     8641 gtatcgccct cctgccattc gcgggtaatg tgcaaatacc ctttacgaat atcctgctcg
     8701 acctcttccc cattcaatat gatctgcggc tgtgtgcacc agtccggcag acgtaaagcc
     8761 agcgtatgac gtaccggctg gggcgattca accgcaatcg tcacctgctc ctgccacgga
     8821 tagttcccgc taacccgcag gcgcagcgtg ccattttcta ccggcacttc catgctgttt
     8881 cctgcgtata tgttgatata caacgcatct tcacgcggcg tgtagagata atgaccaatc
     8941 gaggtcagca cgcgggcgat atttggcgga caacaagcgc agccaaacca acgctggcgg
     9001 atcggtttaa cgtgatcgta gatatggttg aatttcagcg attttggatg tacttccagc
     9061 ggattcacat agaagaaatg tttgccatcc agcgccatgc cgccgagcac ggtgttgtac
     9121 agtgcgcgct ccatcacatc ggcatattga ctgtcgcctt ccatttccag cattcgtcgg
     9181 gcaaacatca tcaggccgat ggaggcgcaa ctttcagcgt ataccgtgtc attcggcaga
     9241 tcgtaatcgc tagtgaacgc ttcgccgctg ctttgcgagc caatgccgcc ggtaatatat
     9301 aactgacgct gggccatatt gttccacagc cgcaggcagt cctgacgctt gctgtcatcg
     9361 tgacttaaac gcgcgagatg cgcgacgccg gtcatcagat agacaaaacg taccgcgtga
     9421 ccgatggcgg tttgctgttg cgcaaggggc aaatgtgcct ggctgtaggc tttgtctttc
     9481 accatccacg ccgggccgta ggtgtgccag tgcgatgtct gcccgcgctt ttcatattcc
     9541 tggtcgtaat agtgcggttg cgcaccacgc tgttcgacaa aatagttcgt cagcgccagg
     9601 tagcgcggct cttcagtcac ttcatacaga cgcatcagtg ccagttcaat ttccgggtga
     9661 ccagggtaac cgtgtaactt actttcatct ggaccaaata cgctgtcgat atgatcggcc
     9721 aggcggcaaa cgacccccag caagcgccgt tttcccgtgg cctggaagaa ggcgactccg
     9781 gcttcaatca gatgacctgc gcagtaaagt tcatgacact ccgccagatt gctccagcgt
     9841 tcttcgggtg cttttaccgt aaagtaagta ttgagatagc cgtcttcgca ttgggcggag
     9901 gcgatcagtt cgattacctc gtcggcggtt ttttccagtt cggcgtccgg cttctggcac
     9961 agcgaccagg ctaccgcttc cagccatttg gcgacgtcgc tgtcctgaaa caccatcccg
    10021 taaaattcac cctcctgaag tccggcggca atgcgaaagt tttcaatcgc atggctgggt
    10081 tccgcttctg ggatacgatc gttcaaggca tcccactgat aaggaatcac cacgtcgcgg
    10141 accagttgtt ggtactgacc gaggaacgga tcgctgaccg tcagtttatg cagatcgact
    10201 tccgaaatgt tcatcttgtg ctccttaagg ctgtacatga cgctgacgca ggtcggtagc
    10261 tatgcgtgac atcataggat tattgagttt gcaaaagcgg atcgcccctg ccagcagcag
    10321 gtggaatagc gcaggcagta atgtttccat cgccgcgatc ccgccaagcg aactggcggt
    10381 ctggttttcc acgccaggct ggtaggcgac aaaaataaac agcaggctga tgatcccggc
    10441 gctggaggcc caggccagct tgatgaaaaa cagattgaag gcgaagttca tgccggaaga
    10501 acgtacgcgg gttttccact cgccgtagtc atcggcaaag gccatcaatg agaagtgcag
    10561 tggcaatgtg aagccaagaa ttacgccatt accgaggatc accgccagcc acagcgtttg
    10621 ataagccggg ccggagggca ggaaccacat caacaccgcc agcgcagcga ggagcaggtt
    10681 ggtgtagtag taaattttga cggtatcgaa acgccgcgtt aacgggctga caatcaccga
    10741 gccgataatg gaggcgaagg tgaccatggt gaagaacagc gacgtgtagc ccgtactgcc
    10801 ttgtaagacg taggtaatga agtacatata cccaccgccg cgaatgttaa agacgttaat
    10861 cagcaggaaa gacatgacca gcatcagcag cagttggtcg ttgtttcgca gcccggcaag
    10921 atgttcgcgc agggtaaatt tccccattgt ggagagtggc acccgttcac gaacccagaa
    10981 aaaacagcac aggaacatca ccacggcaat ggcgcacagc acgccgacgc ccaattgata
    11041 gccgcgcgcg gcattaccct gaccgaagag atctaccatc cacggtaagc ctaccgaaac
    11101 cagaaatccc gctacgccac agagaacgaa tcgccaggac tggcaggaga tcacttcatt
    11161 gtggcgggtg gtcatggtgt tgatcagcgc gcagtacggt acgttgatgg cggtataacc
    11221 gactgaaagg agcaggtaag taccgaacgc ccaggcgatt tttactccca tgctggcatc
    11281 tggcacggtg aacgtcagga tgccgataat gccgatggga atggcaatcc atagctgcca
    11341 cggacgaaag cgtccccagc gactttgcgt ccggtcggca atgacgccca tcaccgggtc
    11401 ggagatggca tcaaacacgc gcagtgcaat aaacagcgtg ccaaccagcg ccggagttaa
    11461 tccaaaaacg tcggtgtaga aaaaggtgag aaaattcatg atcaggcaag ttattacggt
    11521 gccgcctgcg tcacccaggc cgtagcctat tttttcccgt acagataacg catcgtcaat
    11581 tttaccttta gcgatatcag cggtagtaat cggtgtagag gtcatagata ttcctcgtga
    11641 atagggagca atttttagtt ttattgaaat gacctgatta attctgccgt gaacgggaat
    11701 atcaacaagg cgagaaaaga gtaaaaaaag gtgacgattc cgacctgaat ttaaaagtgt
    11761 gaaatggatc aggtgaaatt aatgtttttg attaa
//
LOCUS       DAPGOF010000156        11793 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.157, whole genome shotgun sequence.
ACCESSION   DAPGOF010000156 DAPGOF010000000
VERSION     DAPGOF010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11793
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.157"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            219..1307
                     /gene="pdeL"
                     /locus_tag="RHV13_003706"
     CDS             219..1307
                     /gene="pdeL"
                     /locus_tag="RHV13_003706"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator/c-di-GMP
                     phosphodiesterase PdeL"
                     /protein_id="HDV1243194.1"
                     /translation="MNSCDFRVFLQEFGTTVHLSLPGSVSEKERLLLKLLMQGMSVTE
                     ISQYRNRSAKTISHQKKQLFEKLGIQSDITFWRDIFFQYNPEIISATGSNSHRYINDN
                     HYHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(1349..2281)
                     /locus_tag="RHV13_003707"
     CDS             complement(1349..2281)
                     /locus_tag="RHV13_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV1243195.1"
                     /translation="MNSVFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQIIGEGIGFLPDYMVREAMAQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(2373..2870)
                     /locus_tag="RHV13_003708"
     CDS             complement(2373..2870)
                     /locus_tag="RHV13_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HDV1243196.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALAAGWLASVSGISGFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFACGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            3128..3733
                     /locus_tag="RHV13_003709"
     CDS             3128..3733
                     /locus_tag="RHV13_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HDV1243197.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQPAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            3773..4636
                     /locus_tag="RHV13_003710"
     CDS             3773..4636
                     /locus_tag="RHV13_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HDV1243198.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWQQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQTVNDKQNI
                     ASAVASMMGLGIGLTPSADDYLTGLALILFIPGHPAEKYKEEFYLGLQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            4626..6173
                     /gene="fdrA"
                     /locus_tag="RHV13_003711"
     CDS             4626..6173
                     /gene="fdrA"
                     /locus_tag="RHV13_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HDV1243199.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKAGADNEQLLVEIEELFNSKAQSGSHEARYA
                     TLASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRGETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGEVYSNIQPDPEFRLKDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            6173..7591
                     /locus_tag="RHV13_003712"
     CDS             6173..7591
                     /locus_tag="RHV13_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HDV1243200.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAADLAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPILRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            7641..7982
                     /locus_tag="RHV13_003713"
     CDS             7641..7982
                     /locus_tag="RHV13_003713"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243201.1"
                     /translation="MARESVGLISVAHQAFVTIAGCGVNALSGLRVAREFVGLISVAH
                     QAFVTIAGCGVNALSGPRMAREFVGLISVAHQAFVTIAGCGVNALSGLRMARKFVGLI
                     SVAHQAVWRWS"
     gene            8014..8964
                     /locus_tag="RHV13_003714"
     CDS             8014..8964
                     /locus_tag="RHV13_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HDV1243202.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSDSQRDELQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTDAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPEQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            8974..10356
                     /locus_tag="RHV13_003715"
     CDS             8974..10356
                     /locus_tag="RHV13_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDV1243203.1"
                     /translation="MKENNSRREFLSQSGKMVTAAALFGTSVPLAHAAVSGTTNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQNGKIVALRENKQHPDAT
                     LPHYDAGDKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFVLATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            10733..11782
                     /gene="yahK"
                     /locus_tag="RHV13_003716"
     CDS             10733..11782
                     /gene="yahK"
                     /locus_tag="RHV13_003716"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HDV1243204.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSESKREAAKALG
                     ADEVVNSRNADEMAAHVKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
BASE COUNT         2972 a         2926 c         3210 g         2685 t
ORIGIN      
        1 cgtgattatc tcctgagttt gacttgtgat taccttttta aggtatttag cgtaactgtt
       61 tttgagcgag catcagaggt aaagataatc ttcttgatag tgatgtggga tgttatacgt
      121 atggcatcgc taatgtttat ggttacccct tatgtgtgct caggaatcca caggtaatca
      181 ctcatactga acagcgataa aagataaaag gtgtgttcat gaattcatgt gattttcgtg
      241 tttttctgca agagttcggt acaacggttc atttgtcatt gcctggtagc gtatccgaga
      301 aagaacgact gctactcaag ctgctgatgc agggaatgtc tgtaacagaa atatcacagt
      361 acagaaatcg cagtgcaaag acaatttcac atcaaaagaa acagctcttt gagaaactgg
      421 ggattcagag cgatattact ttctggcgcg atattttctt tcagtacaat ccggagatca
      481 tatccgccac gggtagtaat agtcatagat atattaatga taatcactat caccatatcg
      541 tcacgcctga agccatcagt ctggcgttgg aaaaccacga attcaaaccg tggatccaac
      601 cggttttctg cgcgcagact ggggtactga cgggctgtga ggtgcttgtc cgctgggaac
      661 atccacaaac gggaattatc ccaccggatc agtttattcc tctggcggag tcatccggtc
      721 ttattgtcat aatgacccgc caactgatga aacagactgc ggatattctg atgccggtaa
      781 aacatttgct gccggataat ttccatattg gtatcaacgt ctcggcgggt tgttttttgg
      841 ccgcgggatt tgaaaaagaa tgcctgaacc tggtcaagaa attaggtaac gataaaatca
      901 agctggttct ggaactcacc gaacgtaacc caatccctgt tacgccggaa gccagagcga
      961 tatttgacag ccttcatcag cataacatta cctttgcgct ggatgacttt ggtacgggtt
     1021 atgcgaccta tcgttatctg caggcgtttc cggtcgattt tattaagatc gataagtcat
     1081 ttgtgcaaat ggcgagtgtc gacgaaatat ccggtcatat tgtggacaat attgtcgaac
     1141 tggcgcgtaa gcctggtctg agtatcgtgg cggaaggggt agaaacccag gagcaggcgg
     1201 atttaatgat cggtaaaggc gttcactttt tgcagggcta tttgtactct ccgccagtac
     1261 cgggtaataa atttatctct gaatgggtaa tgaaagcagg tggttgatgt aaaccgctat
     1321 tcacagcgca tcgggaggtt ggcagcgatt aattctcccg atgcagtaaa tcctgataga
     1381 tccccgttaa tatgccattt ggcgcaaatt gttttttaat ccactgcgtc acttggccgg
     1441 tggcagagtg ctgggttgcg agtaacatgc gtgaatcctg gcgcgggtta tggatttgtc
     1501 gggtaaccag caaggattgc gccatcgcct cacggaccat gtaatccggc aaaaaaccaa
     1561 ttccttcacc tataatctgg cactgacatt tggtgttgaa atctggcacc agaattgact
     1621 cctgcccatg cagcaaccag cccacttttt tattaatcgt atgtgcggtg tcctccacca
     1681 tgatattagg atagagacgc agctggcttt cggcgatggg ttccggcaca aaggctaacg
     1741 ggtgatccgg ggcgatagca aaagcccagc gaatcgcgcc aatttcagtg taatcaatac
     1801 cgccgccgtc cagtagtgta tcgggtgcgc cgatggcgat attggcctga ttattaataa
     1861 tcgcatccca gacgccgtta tacacttcgg tcgtcacggt aatctggcag gtaggaaact
     1921 gctttttcag cacctgtaat aagcgcgcgg tgtgtttggg cgtataaagc agctgattaa
     1981 tacaaatgcg cacacgcgct tctatgccct gcgaaatagt atctatgcta cgtttgatgg
     2041 cataaaaatc attcagcagg tcggtagctt tgcggaaaaa ataacgcccg gactccgtta
     2101 actcaatgct gcgggtactg cgagtgaaca gcaccacatc cagccctgtt tccattcgct
     2161 tgatggtgta gctaatggcg gaagtggtta aacccaactc ttctgcggct ttactgaaac
     2221 tgccaaaacg cgcagcggta gtaaatgcca acagattctc ttcggtaaaa actgagttca
     2281 ttaaaccatt cccgccatta tctaatatga atttctccag ccatgactgt agaggtatcg
     2341 attaaagata gataatcatt tttgaataac ttttaatacc cgtcgcgttt agtcgtcgct
     2401 tcgcttaatt tttgtccgcc atattctgaa attatcccca cgcagttacc cgccagaaaa
     2461 cccagtaacg tcaccgtcca gttcatcccg caggcaaaaa acagcgtcat gccgagaaag
     2521 ccaccgggaa tgaatgacag taaccagaaa cgtccttgcc agaccactac cgctgagaaa
     2581 ggcacggtag taatcacgct cgcccagaac ccagaaatcc cactcaccga ggccagccaa
     2641 ccggcagcca gcgcggcgaa aaatgcccac accatcccgg aataattaac cgccagactt
     2701 ctgataaacc ccgatttacc gccgcctgcg gcataaaaac tgcaaaaggc gacgaagcca
     2761 atggttccta ataattccca tccctgagag agcccgacat gtgaagacac taactgccaa
     2821 agccctgcac aaataccgac ggtgacaccc gttgccgtga gtccgttcat cttgttcccc
     2881 tctggttaga catggacgaa agctactctt acatgtgcag actatagaga aataaaacct
     2941 gattgagtga aagggtatcg ggtcaaagaa acaaatattg cactaccgca cactgcgaaa
     3001 agattgttga acatcattca acaaaaaggc gaaatacagc gaattaaatt taagcgggtt
     3061 cttttatcag attatcgttg ctattctcaa ttcatcgaaa acagccacaa aaaccagggg
     3121 aatgtgaatg agtataaaaa atctacctgc cgactattta ttggctgcac aacagggtga
     3181 tatcgataaa gtaaaaacct gccttgcgct gggtgtcgat attaatacct gcgatcgtca
     3241 ggggaaaacg gcaattacgc tggcaagttt atatcagcaa tatgcttgcg ttcaggcatt
     3301 aattgatgcc ggagcggata ttaataagca agatcatacc tgtttaaatc cttttttaat
     3361 tagctgtctg aacgatgatt taacgctact acgaattatt ttacccgcta aacccgatct
     3421 taattgcgta acccgttttg gcggtgtcgg cctgacgcct gcctgtgaaa aaggccattt
     3481 aagtattgta aaagagcttt tggcgcatac ggaaattaac gttaaccaga ccaaccatgt
     3541 aggctggacg ccgctactgg aagcgattgt gcttaatgat ggtggtatta aacagccggc
     3601 gattgtgcag ttattgctgg aacacggtgc cagcccgcat ctgacggata aatatggcaa
     3661 aacgccactg gaactggcgc gggaacgggg ctttgaagag attgcgcagt tactgattgc
     3721 cgcaggtgca taaaccggga ggcttgcaat caacacacca gaaaaacggt gtatgtgggc
     3781 gctaactgcg gatgcggatt ttctggcgca gcgggggcaa ggacaggttg aacaggtctt
     3841 tgccagagcg gtaaatatcg cactcccggc tcgccagcag ttgctgacgc tgctttgtga
     3901 agagtacgac aatgcgccaa acagttgtcg gttggcactc actcactttg atgatctgtt
     3961 ccggcatggt gataaggttc agtttgacga tcaaggtatt acggttggtc aacatcttca
     4021 tatagagatg agtcgttgtc ggcgttggct gtccccaacc ttgcaaatga ccgctgtgaa
     4081 ttttcacctt atcgcctggc aacagtggca cgacattatt catcagcacc tgggggaaaa
     4141 tgaaaccctg tttaattatc gcggcgataa tccgttttat caggcgttaa ataaagaatt
     4201 acatattaaa cgacgggcag ttattcagac agtaaacgat aagcaaaata tcgcctcagc
     4261 ggtcgccagt atgatggggt tagggattgg ccttacgcca tcagccgacg attatttaac
     4321 aggtctggcg cttattttat ttattcccgg gcatccggcg gaaaaataca aagaggaatt
     4381 ttatctcggt ctgcaacgcg gcagaaataa taccacatta ttaagtgcta taacgctgga
     4441 agccgcatta caacaacgtt gccgggaaaa tattcatcgt tttattcaca acattattta
     4501 tgacatccct gggaacgcaa ctcaggcaat agaaaaaatt aaacatattg gctccagttc
     4561 cggctgcgac atgctgtatg gcatggccga tggttgtgcg ctgagccaaa cctacggagg
     4621 gaattatgtc agttaaaata gtcattaaac cgaataccta ttttgattct gtctcgctga
     4681 tgtctatctc cacgcgtgca aataaactcg atggcgtcga gcaggcattt gtggcgatgg
     4741 cgaccgaaat gaacaaaggc gtgctgaaaa atttagggct gctgacgccg gagcttgagc
     4801 aggcgaaaaa cggcgacctg atgattgtca ttaacggcaa agcgggcgcg gacaacgagc
     4861 agttgctggt agagattgaa gaactgttca acagcaaagc gcaaagtggc tcgcacgagg
     4921 cgcgttacgc cacccttgcc agcgccaaaa agcatatccc ggaaagtaac ctggcggtga
     4981 tttcggtcaa cggtctgttt gccgctcgcg aagcgcgtca ggcgctgcaa aacgatctca
     5041 acgtgatgct gttttccgat aacgtctcag ttgaagatga actggcgctc aagcaactgg
     5101 cccacgaaaa agggctgctg atgatggggc cagactgtgg cacggcgatt atcaacggcg
     5161 cggcgctctg ttttggtaac gccgtgcgtc gcggcaacat cggcattgtt ggcgcatccg
     5221 gcaccggtag ccaggaactg agcgtccgta tccatgaatt tggcggcggc gtttcgcaac
     5281 tcatcggcac cggcgggcgc gacctgagcg agaaaatcgg cggcctgatg atgctcgacg
     5341 ccatcgggat gctggaaaac gatccgcaaa ctgaaattat cgcgctgatt tcaaaacctc
     5401 ccgcgcctgc ggtggcccgt aaagtgctgg aacgcgcccg cgcctgccgc aagccggtgg
     5461 tggtctgctt cctcggtcgt ggcgaaacgc cggttgacga gcaggggcta cagtttgctc
     5521 gcggcagcaa agaagcggcg ctaaaagcgg tgatgctctc cggcgtgaaa caggaaaatc
     5581 tcgacctgca tacgcttaac cagccgttga ttgcggatgt gcgtgcgcgt ctgcaaccgc
     5641 agcagaaata cattcgtggc ctgttctgcg gcggcacgtt gtgcgacgaa accatgttcg
     5701 cggtgatgga aaaacatggc gaagtctaca gcaacattca gcccgatccg gaattccgcc
     5761 tgaaagatat caaccgcagc atcaaacaca ccttcctcga ctttggcgat gacgacttca
     5821 ccaacggcaa gccgcatcca atgattgacc ccaccaaccg catcagtcgc ttgatcgaag
     5881 aggcgcgcga tccagaagtg gcggtgatcg tgatggattt tgtgctcgga tttggatcgc
     5941 atgaagatcc ggtcggctcc accatcgagg cgatcaaaga agcgaaagcg atcgccgctg
     6001 ccgaaggacg cgagttgatc attctcgcct atgtgctggg taccgatctt gatacgccat
     6061 cgttagaaca acaaagccag atgctgcttg atgccggagt gattctggcg agcagcagca
     6121 ccaataccgg attgctggcg cgtgaattta tctgcaaagg ggaggaagcc tgatgagcca
     6181 gtcactgttt agccaaccat tgaacgttat taacgtcggc atcgccatgt ttagcgatga
     6241 cctgaaaaag cagcatgtag aagtgactca actcgactgg acgccgccgg ggcagggcaa
     6301 tatgcaggtg gtgcaggcgc tggataacat tgccgattcg ccgctggcag acaaaatcgc
     6361 cgccgctaac cagcaggcgc tggagcgcat tatccagtcg catccggtgc tgattggttt
     6421 tgatcaggcg attaacgtgg tgccgggcat gacgccgaaa accattcttc acgccgggcc
     6481 gccgatcacc tgggaaaaaa tgtgcggcgc gatgaaaggc gcggtcaccg gagcgctggt
     6541 gttcgaagga ctggcgaaag atctcgacga ggcggctgac ctggcggcct ccggggagat
     6601 caccttctcg ccgtgccacg aacacgactg tgtgggatcg atggcgggtg ttacctcggc
     6661 ctcgatgttt atgcacatcg taaaaaacaa aacctacggc aacatcgctt ataccaacat
     6721 gagcgagcag atggcgaaga ttttgcgtat gggcgctaac gaccagagcg tgattgaccg
     6781 cctgaactgg atgcgtgatg tgcagggacc aatactgcgc gacgcgatga aaattatcgg
     6841 cgaaatcgat ctgcgcttaa tgctggcgca ggcgctgcat atgggcgatg agtgccataa
     6901 ccgcaataac gccgggacga cgctgctgat tcaggcgctg acgccgggga ttattcaggc
     6961 gggctactcc gtcgagcaac agcgcgaagt gtttgagttt gtcgccagca gcgactactt
     7021 ctccggcccg acgtggatgg cgatgtgtaa agcggcgatg gatgcggcgc acggcatcga
     7081 atacagcacc gtcgttacca ccatggcgcg taacggcgtc gagttcggcc tgcgggtcag
     7141 cggcctgccg gggcagtggt ttaccggccc agcgcagcag gtcatcggcc cgatgtttgc
     7201 tggttataag ccggaagatt cggggctgga tatcggcgac agcgccatca cagaaaccta
     7261 cggcattggc ggatttgcca tggcgaccgc gcctgcaatc gtcgcgctgg tgggcggcac
     7321 ggtggaagag gctatcgatt tctcccgcca gatgcgtgaa atcacccttg gcgaaaaccc
     7381 caacgtcacc attccgctgc tcggttttat gggcgtgccg tcggcaatcg acatcacccg
     7441 cgtgggtagc agcggcattc tgccagtgat caacaccgcc atcgcccata aagatgcggg
     7501 cgtcggcatg attggcgcgg gcattgtgca tccgccattc gcctgcttcg agaaagccat
     7561 tctcggctgg tgcgaacgtt acggcgtctg acctacatgt tcatgccgga tgcggcgtga
     7621 acgccttatc cggcctacga atggcacgag aatctgtagg cctgataagc gtagcgcatc
     7681 aggcatttgt caccattgcc ggatgcggcg tgaacgcctt atccggccta cgagtggcgc
     7741 gagaatttgt aggcctgata agcgtagcgc atcaggcatt tgtcaccatt gccggatgcg
     7801 gcgtgaacgc cttatccggc ccacgaatgg cacgagaatt tgtaggcctg ataagcgtag
     7861 cgcatcaggc atttgtcacc attgccggat gcggcgtgaa cgccttatcc ggcctacgaa
     7921 tggcacgaaa atttgtaggc ctgataagcg tagcgcatca ggcagtctgg cgttggtcat
     7981 aaccccatca ccctctgtag cggacataac aacatgaaag agcttgtggt cgttgccatt
     8041 ggtggcaaca gcattatcaa agataacgcc agccagtcga ttgagcatca ggcggaggcg
     8101 gtgaaagccg tcgccgatac ggtgctggaa atgctggctt ccgattacga cattgtgctg
     8161 acccacggta acgggccgca ggtcgggctg gatttacgcc gtgcggagat tgcccacgag
     8221 cgcgaagggc tgcccttaac gccgctggcg aactgtgtgg cggatacgca aggcggcatt
     8281 ggctatctga tccaacaggc gctgaacaac cgactggcgc gtcacggcga gaaaaaagcc
     8341 gtcaccgtgg tgactcaggt ggaagtggat aaaaacgatc cgggatttgc ccatcccacc
     8401 aagcccatcg gcgcattctt tagtgacagc cagcgtgacg aattacaaaa ggcaaaccct
     8461 gactggtgtt ttgttgaaga tgccgggcgg ggctatcgcc gcgtagtcgc ttcgccggaa
     8521 ccgaaacgta ttgtcgaagc accggccatt aaggcgctga tccaacaagg ctttgtggtg
     8581 attggcgcgg gcggtggcgg aattcctgtg gtgcgtactg acgcgggaga ttaccaaagc
     8641 gtggacgcgg ttatcgacaa agatctctct accgcgctgc tggcccgtga aattcacgcc
     8701 gacattctgg tgatcaccac cggggtggaa aaagtgtgta ttcattttgg caaacccgag
     8761 cagcaggcgc tggatcgggt ggatattgcc accatgactc gctatatgca ggaaggacat
     8821 ttcccgcccg gcagcatgtt gccaaaaatc atcgccagcc tgacgttcct ggaacagggc
     8881 ggtaaagaag tgattatcac cacgccggaa tgcctgccag cggcgctgcg cggtgaaacg
     8941 ggcacccaca ttattaaaac gtaaggacgt aagatgaaag aaaacaatag ccgccgtgag
     9001 tttttgagcc agagcggtaa gatggtcacc gccgccgcgc tgtttggtac ctctgtgccg
     9061 ctcgcccatg cggcggtctc tggcaccaca aactgcgaag cgaacaacac catgaaaatc
     9121 actgacccgc attactatct cgacaacgtg ctgctggaaa ctggatttga ctacgaaaat
     9181 ggcgtggcgg tgcagacccg cacggcgcgc cagaccgtgg agattcagaa cggcaaaatt
     9241 gtcgcgctgc gcgagaataa gcagcacccg gatgccacgc tgccgcacta tgacgctggc
     9301 gataagctga tgctgcccac cacccgcgac atgcatattc atctcgacaa aaccttctac
     9361 ggcgggccgt ggcgctcgct caatcgtccg gcaggcacca ctatccagga catgatcaaa
     9421 ctcgagcaga aaatgctgcc ggagttacaa ccgtatacgc aggagcgggc ggaaaaactg
     9481 atcgatctat tgcagtcgaa aggcaccacc attgcccgca gccactgcaa tatcgaaccg
     9541 gtttccggtc tgaaaaatct gcaaaatttg caggcggtgc tggcgcgacg tcaggcgggc
     9601 ttcgagtgtg aaattgtcgc cttcccgcag cacggtttgc tgctgtcgaa atcggaaccc
     9661 ttaatgcgtg aagcaatgca ggcgggtgcg cattacgtcg gcgggctgga cccgaccagt
     9721 gtcgatggcg cgatggaaaa atccctcgac accatgttcc agattgcgct ggactacgac
     9781 aaaggcgtcg atattcacct gcacgaaacc actccgtcgg gcgtggcagc catcaattat
     9841 atggttgaaa cggtagagaa aacgccgcaa ctgaaaggta agctgaccat cagccacgcc
     9901 tttgtgctgg ctacgctcaa cgaacaacag gtcgatgcgc tggcgaatcg gatggcggcg
     9961 cagcaaattt ctatcgcctc gacggtgccg attggcacgc tgcatatgcc gctcaaacag
    10021 ttgcacgaca aaggcgtaaa agtgatgact ggcactgaca gcgttatcga ccactggtcg
    10081 ccttatggtc tgggcgacat gctggaaaaa gccaatctct acgcgcagct ctatattcgt
    10141 cctaacgaac agaacctctc ccgttcgctg tttttagcca ctggcgatgt attgccgctg
    10201 aatgaaaaag gcgagcgtgt atggccaaaa gcgcaggatg acgccagctt tgtgctggtg
    10261 gacgcctcct gttccgccga ggcggtggcg cgtatctcgc cgagaaccgc aacgttccat
    10321 aaagggcaac tggtgtgggg gagtgtggca ggttgatgcg aatttgatat gtctgcaacg
    10381 gtgaacggta agagatttaa gccccaggga gtggggcaat ctgaatatga gtgtgtcggc
    10441 agaaatggac attatctttt aggaatatgc ccttattgac gttgtgtaga taactatttg
    10501 tctacgtgaa aagatcatca gttttgccga ttttagtctt ttacggatag caaatatcac
    10561 acttataggc gctcgcctta tggggaacgt tgctgctctg tttcattcag gctcgcggca
    10621 tgttggggct ggatcgggtg gtttaaatat tggctatatt caatggacgc gttttgccgc
    10681 gatgacatat caggcgtcgc caaatacaca tagctaatca ggagtaaaca caatgaagat
    10741 caaagctgtt ggtgcatatt ccgctaaaca accacttgaa ccgatggata tcacacggcg
    10801 tgaaccggga ccgaatgatg tcaaaatcga aatcgcttac tgtggcgtct gccattccga
    10861 tctccaccag gtccgttccg agtgggcggg gacggtttac ccctgcgtgc cgggtcatga
    10921 aattgtgggg cgtgtggtag ccgttggtga tcaggtagaa aaacatgcgc cgggcgatct
    10981 ggtcggtgtc ggctgcattg tcgacagttg taaacattgc gaagagtgtg aagacgggct
    11041 ggaaaactac tgtgatcaca tgaccggcac ctataactcg ccgacgccgg acgaaccggg
    11101 ccatacgctg ggcggctact cacaacagat cgtcgttcat gagcgatatg ttctgcgtat
    11161 tcgtcacccg caagagcaac tggcggcggt ggcccctttg ttgtgtgcag ggatcaccac
    11221 gtattcgccg ctacgtcact ggcaggccgg gccgggtaaa aaagtgggcg tggtcggcat
    11281 cggcggtctg ggacatatgg ggatcaagct ggcccacgcg atgggggcgc atgtggtggc
    11341 atttaccact tctgagtcaa aacgcgaagc ggcaaaagcc ctgggggccg atgaagttgt
    11401 taactcacgc aatgccgatg agatggcggc tcatgtgaag agtttcgatt tcattttgaa
    11461 tacagtagct gcgccacata atctcgacga ttttaccacc ttgctgaagc gtgatggcac
    11521 catgacgctg gttggtgcgc ctgcgacacc gcataaatcg ccggaagttt tcaacctgat
    11581 catgaaacgc cgtgcgatag ccggttctat gattggcggc attccagaaa ctcaggagat
    11641 gctcgatttt tgcgccgaac atggcatcgt ggctgatata gagatgattc gggccgatca
    11701 aattaatgaa gcctatgagc gaatgctgcg cggtgatgtg aaatatcgtt ttgttatcga
    11761 taatcgcaca ctaacagact gaaaaaattt ata
//
LOCUS       DAPGOF010000157        11675 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.158, whole genome shotgun sequence.
ACCESSION   DAPGOF010000157 DAPGOF010000000
VERSION     DAPGOF010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.158"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            64..357
                     /locus_tag="RHV13_003717"
                     /pseudo
     CDS             64..357
                     /locus_tag="RHV13_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(415..846)
                     /gene="tnpA"
                     /locus_tag="RHV13_003718"
     CDS             complement(415..846)
                     /gene="tnpA"
                     /locus_tag="RHV13_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element IS609 family
                     transposase"
                     /protein_id="HDV1243205.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTKYRRQIFDHDATEK
                     LRTYFSKVCADFEAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGVSGRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCGGAPISAIRQYIEQQQTPG"
     gene            854..2062
                     /locus_tag="RHV13_003719"
     CDS             854..2062
                     /locus_tag="RHV13_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV1243206.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNVTETQWLKDAPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPAHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQACGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQ
                     EPTEMIQATA"
     gene            2106..2213
                     /locus_tag="RHV13_003720"
                     /pseudo
     CDS             2106..2213
                     /locus_tag="RHV13_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(2197..2835)
                     /gene="ytfB"
                     /locus_tag="RHV13_003721"
     CDS             complement(2197..2835)
                     /gene="ytfB"
                     /locus_tag="RHV13_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HDV1243207.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            3053..3673
                     /gene="fklB"
                     /locus_tag="RHV13_003722"
     CDS             3053..3673
                     /gene="fklB"
                     /locus_tag="RHV13_003722"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDV1243208.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            3982..5394
                     /gene="cycA"
                     /locus_tag="RHV13_003723"
                     /gene_synonym="dagA"
     CDS             3982..5394
                     /gene="cycA"
                     /locus_tag="RHV13_003723"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HDV1243209.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(5439..6101)
                     /gene="ytfE"
                     /locus_tag="RHV13_003724"
     CDS             complement(5439..6101)
                     /gene="ytfE"
                     /locus_tag="RHV13_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HDV1243210.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIETELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(6209..7174)
                     /locus_tag="RHV13_003725"
     CDS             complement(6209..7174)
                     /locus_tag="RHV13_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDV1243211.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLLQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLACVNIAELNHGLPNFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLVFISLMIAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVVGVVIAVRAKPEKPLTESVSES"
     gene            complement(7282..8142)
                     /gene="qorB"
                     /locus_tag="RHV13_003726"
     CDS             complement(7282..8142)
                     /gene="qorB"
                     /locus_tag="RHV13_003726"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HDV1243212.1"
                     /translation="MIAITGATGQLGQYVIESLMKTVPASQIVAIVRNPAKAQALTAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGHPTTTLAE
                     SVSHLFNVNN"
     gene            8231..8611
                     /locus_tag="RHV13_003727"
     CDS             8231..8611
                     /locus_tag="RHV13_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDV1243213.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKMGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(8740..10683)
                     /gene="cpdB"
                     /locus_tag="RHV13_003728"
     CDS             complement(8740..10683)
                     /gene="cpdB"
                     /locus_tag="RHV13_003728"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HDV1243214.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLTAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIA
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            10873..11613
                     /gene="cysQ"
                     /locus_tag="RHV13_003729"
     CDS             10873..11613
                     /gene="cysQ"
                     /locus_tag="RHV13_003729"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HDV1243215.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
BASE COUNT         2759 a         2937 c         3043 g         2936 t
ORIGIN      
        1 tgcggtatgt aattattcta cgctgtcggt atcggtgtat tcgttaatca gcaggttaac
       61 cagccacaaa aagccattga gaaaattatt gattttacat gggattatta tattgctaat
      121 tcctggtttt taaaaattgt gcatagtgaa aatcaaagta aaggtgtgca ttatgcaaaa
      181 tcacagcgtc tcttagaaat tacccatgcg cacttgcggt taatggaatc cttattggat
      241 gaagggaaga aacacaatat ttttaagcca gatatcgacc cattgcaggt gaatattaat
      301 attgctgcgc ttgggggata ttatttgatc aaccagcata cgcttggcct ggtttatccg
      361 gtgcgccgta aaaccccgtc cttcagggcg gggatatacg gcgcggtttt ccacctaacc
      421 aggtgtttgc tgttgttcaa tgtattgacg gatggcggat attggcgcac ctccgcagct
      481 actggcaaaa tagccaggac tccacaaaac gcctttgtag taatacctga ctgcaatatc
      541 tggtcgatct cgtcgcagta acctacccga tacgcctttg aggctgttta ccagactgga
      601 tatcgccagt ttgggaggat agttaattaa caaatggacg tgatctggtt cgccatccat
      661 ttcaaccagt tcagcttcaa aatcagcaca tacctttgaa aagtaagtgc gtagtttttc
      721 tgtcgcgtca tggtcaaaaa tctggcgtcg gtatttggtg acaaagacca ggtgaacatg
      781 catcaggaaa acacaatgcc ttccacgccg gatatcggtt tcttttttca cagaccaaag
      841 tatcatttgg cctgtgaaac gactacaggc atttaaattc cagttaagac ccggtggtca
      901 acaggagcgt gaaatgaggc gcttcgcagg cgcatgtcgt ttcgttttca atcgtgctct
      961 ggcgcttcag aatgagaatc atgaggccgg taataaatac atcccttacg ggaaaatggc
     1021 ttcctggctg gttgagtgga aaaatgtcac tgaaacgcaa tggcttaaag atgccccgtc
     1081 acagccattg caacagtcac tgaaagacct tgagcgggct tacaaaaact tcttccggaa
     1141 gcgggctgct tttccccgat tcaaaaagcg gggacagaat gatgcattcc gctacccgca
     1201 gggcgttaag ctcgatcagg aaaacagccg tatttttctg ccgaaactgg gctggatgcg
     1261 ctaccggaat agccgtcagg tcacgggtgt tgtgaaaaat gtcactgtca gccagtcctg
     1321 cggtaagtgg tacatcagta ttcagacaga aagtgaagta tccactccgg ctcacccttc
     1381 agcatcaatg gtcgggctgg atgctggcgt ggctaaactc gccacgctgt cagatggcac
     1441 agtctttgag cctgtaaaca gttttcagaa aaaccagaag acgctggcga gacttcagcg
     1501 acagttaagc cgcaaggtca aattcagcaa caactggcag aagcagaaac gcaaaataca
     1561 gcgactgcat tcctgtatcg caaatatccg cagggactac cttcacaaag tcacaacgac
     1621 cgtcagcaaa aaccacgcaa tgatagtcat tgaggatttg aaggtcagca acatgtcaaa
     1681 gtcagcagcg ggtacggtca gccagccggg gcgcaatgtc cgggcaaaat caggtttaaa
     1741 ccgttcgata ctggatcagg gctggtatga aatgcgccgc cagcttgagt acaagcagct
     1801 ctggcgtggc ggtcaggtgc ttgctgttcc gccagcgtac acaagccagc gttgcgcgtg
     1861 ctgtggtcat acagcgaaag aaaatcgcct gtcacaaagt aaattcagat gccaggcatg
     1921 tggatataca gcgaacgctg atgtaaatgg cgctcgtaac attttagcgg cggggcacgc
     1981 cgttcttgcc tgtggagaga tggtgcagtc aggccgcccg ttgaagcagg aacccaccga
     2041 aatgattcag gcgacagcct gaacgtagca gggatccacg tccttcaggg cgtggaggat
     2101 gtcaacacat cagtatggtt tcaccccagg cactggaagc cagacgtaag gtcatcaaag
     2161 agacaatcct tagctggctt ttggttgacc cttcatctac cgcgcacgaa taaaactgcc
     2221 atccggctgg cgggtgaaca ggacctgttg attattcccc gtatcaatgg ttaagcccgt
     2281 caccacgccg ctggcgttct ggcggatttt caccatctgc ccgttttgca aattactcag
     2341 cggcttaccc gcgccttcta cctgcgccat tgcatagaca tcggtagcgg gcagtccgtg
     2401 gtcgcggaac agctgcgcca tcgttttacc gggttcgaca cggtaagagc gccattgatt
     2461 atcaattccg ctatctggct ggaatggttg cgtttgggta gtttgcggct gttcttctgg
     2521 ctggccttcc tgtataggtt ctggagcgac cggagccacc tgatcaggat cattttgtgg
     2581 ggtgactaat tgcgcccgca gctgttcttc tgtcggcggt tgtgattgcg actgaatatc
     2641 taactgcgct tcacgcgtca ccaccggcgc gttgggcgta tcatcagatg gaagcagaaa
     2701 tccgaccacc aatacaatgg cggcaataat gatcccccga cgatgcatcg gcggcagcgg
     2761 gtccatgaag cgaaaattgt ccggcgcgtg ccagactttc tccagggttg gttttagttc
     2821 aaagcgcccg ggcatggttt cctcctgctc cgcgtcttgt tcctcaatca tagcctatga
     2881 ataagctaac gctatgatgt ccgtggtaaa cccgccttta ttatattcat acgggatatt
     2941 gctattgttt ctttttccct gggatttgtc atcattcccg cgacaaagtt ttacccaaag
     3001 aagtgtggct gatatgctgc ccgctacttt ataccctaag aaaggaaata cgatgaccac
     3061 cccaactttt gacaccatcg aagcgcaagc aagctacggc attggtttgc aggtagggca
     3121 acaactgagt gaatctggcc tggaagggct gctgccagaa gcactggttg caggtattgc
     3181 cgatgcgctg gaaggcaaac atccggctgt tccggttgat gtggtgcatc gcgcactgcg
     3241 tgaaatccac gaacgcgccg atgccgttcg tcgtcagcgt ttccaggcga tggctgctga
     3301 aggtgtgaaa tacctggaag aaaacgccaa aaaagaaggt gtgaatagca ccgaatctgg
     3361 cctgcaattc cgcgtgatca accagggtga aggcgcaatt ccggcacgta ccgaccgcgt
     3421 tcgtgttcat tacaccggta aactgatcga cggcaccgtg tttgacagct ccgttgctcg
     3481 tggtgaaccc gctgaattcc cggttaatgg cgtgatccct ggctggattg aagcactgac
     3541 tctgatgccg gtaggttcta aatgggaact gactatcccg caggaactgg catatggcga
     3601 gcgcggcgca ggcgcatcca tccctccgtt tagcaccctg gtgtttgaag tcgaactgct
     3661 agaaatcctc taagcagcgc attctgttcc cctcgaacca gaggggagca ggcattcagc
     3721 aataaaccct tcagtttgcc aaacggcgct attttgtgtt gcaaagaccc cgtaagcgtg
     3781 tatttttgtg agctgtttcg cgttatcacc gtgatatgac actcacttta aacataaaat
     3841 taacattaga tctaaatctt agtattcatc ccgcgtattg ttacctaata tcgatgagtc
     3901 ccgatacaga ttcgtcgtat catagactga ctaaaggccg tagagcctga acaacacaga
     3961 caggtacagg aagaaaaaaa catggtagat caggtaaaag tcgttgccga tgatcaggct
     4021 ccggctgaac agtcgctacg gcgcaatctc acaaaccgac atattcagct tattgccatt
     4081 ggcggtgcca ttggtacggg gttgtttatg gggtccggca aaacgattag ccttgccggg
     4141 ccgtcgatca ttttcgttta tatgatcatt ggttttatgc tctttttcgt gatgcgggca
     4201 atgggggaat tgctgctttc gaatctggaa tacaaatctt ttagtgactt cgcttccgat
     4261 ttactcgggc cgtgggcagg atatttcacc ggctggactt actggttctg ctgggttgta
     4321 accggtatgg cagacgtggt ggcgatcacg gcttatgctc agttctggtt ccccgatctc
     4381 tccgactggg tcgcctcgct ggcggtgata gtgctgctgc tgacgcttaa cctcgccacc
     4441 gtgaaaatgt tcggtgagat ggagttctgg tttgcgatga tcaaaatcgt cgccatcgtg
     4501 tcgctgattg tcgtcggcct ggtcatggtg gcgatgcact ttcagtcacc gactggtgtg
     4561 gaagcgtcat tcgcgcattt gtggaacgat ggcggctggt tcccgaaagg tttaagtggc
     4621 ttctttgccg gattccagat agcggttttc gctttcgtag ggattgagct ggtaggtaca
     4681 acagctgcgg aaaccaaaga tccagagaaa tcactgccac gcgcgattaa ctccattccg
     4741 atccgtatca ttatgttcta cgtcttcgcg ctgattgtga ttatgtccgt gacgccgtgg
     4801 agttcggtag tcccggagaa aagcccgttt gttgaactgt ttgtgttggt aggtttgcct
     4861 gcggctgcca gcgtgatcaa ctttgtggtg ctgacctctg cggcgtcttc cgctaacagc
     4921 ggcgtcttct ctaccagccg tatgctgttt ggtctggcgc aggaaggtgt ggcaccgaaa
     4981 gcgttcgcta aactttctaa gcgcgcagta cccgcgaaag ggttgacttt ctcgtgtatc
     5041 tgtctgctcg gcggcgtagt gatgttgtat gtgaatccta gcgtgattgg cgcgttcacg
     5101 atgattacaa ccgtttccgc gattctgttt atgttcgtct ggacaattat cctttgctcg
     5161 tacctggtgt accgcaaaca gcgtcctcat ctgcatgaga agtcgatcta caagatgccg
     5221 ctcggcaagc tgatgtgctg ggtatgtatg gcgttctttg tgttcgtggt cgtgttgctg
     5281 acactggaag atgacactcg ccaggcgctg ctggttaccc cgctgtggtt tatcgcgctg
     5341 gggttgggct ggctgtttat tggtaagaag cgggctgctg aactgcggaa ataaccgcat
     5401 tatcatgctg gatggcgcaa tgccatccag cttttagatc actcacccgc cagggcgcgt
     5461 gggaacagta cattgttttc cagactgatg tgatccatca ggtcatcaat cagttcatta
     5521 atgccgttat acatcgcttt ccaggtggtg caggcttctg gcggcggtgt gacgttattg
     5581 gtagtgtgtt tgatcacttc cagcagttcg cccgcttcat cgtgctcgct ttccattacg
     5641 ctgattggcc ccattgcctg gctgcccatg ccttgtttga tcatcgggaa gagaatctgc
     5701 tcttctttca tcatgtggct ggaaagctct tcatgcagca tagtcaggta ttttgtcagc
     5761 ccttttggca cgctcggttt gtcggcgtga acgcgctcga ctttagtcgc ttgcaggatc
     5821 agctccggca gttgctcgcg gtgacgatcg tggtagcgca cgatgatatg gtcaatgatt
     5881 tctgccagcg gggcgctacg ccagtctttc tcaatcggtt gttcagcaag ctttgccagt
     5941 tcagtttcaa tgacctcaac atccagttct ttacgtgccg ccgcgcgcgc cagcgtttgc
     6001 ttaccgccgc agcagtaatc catatcatat ttacgaaaca gagctgaagc gcgaggaata
     6061 gagagcgcca gttcacctaa aggttggtcg cgataagcca tagctgatac ctcattctta
     6121 ataatataag atgtatttta aatgcatctt taaggcaaat gctataaccc ttacgtagca
     6181 gaggcttatt taactcacag ccagcatgtc aactttctga gacagattca gttaaaggtt
     6241 tttctggttt tgctctgaca gcaatgacca cgccaaccac taacagcgcg ataccgctca
     6301 gcgtcattag cggcggcatt tgctggcgga gtaaaaaggt gtacagcaaa cctgccagcg
     6361 tttcgaaaac aatcagcggc ccgagaatca ctgtcggtaa tcgctggctg gcgacgttcc
     6421 agcagagtgc gccaacccat gagcaaagca cggctatcgc aatcatcaga ctaataaaca
     6481 ccagcggacg ggggccaaag ggtagggaga agtccggcgt ttgcgtattc agccagtaac
     6541 aagcgacgag atagccgata agtgaaaccg gcagtgtgac cagcgcctgc gctgttgccc
     6601 acatcatcgg atgtttgtcg ggattttccc gcagccagcg ggcgttgcgc agggcatacc
     6661 acgcccagca gaccacggaa actaacgcca gcacgatgcc agaggtataa cgtgcccagt
     6721 caaaattggg gagtccgtgg tttaactcag caatattcac acacgccagg ccgatgccaa
     6781 tacaaatcag tgccggggcg agttttcccc acgcgagttt gccgtcgcgc tggctataaa
     6841 gcagattggc aaagacagga atcaccaccg gcagggtgcc gataatcatc gtggaaacag
     6901 gcgcgccagt acgttgaatg gcactggcaa ggcagaagta atagatgagg ttgcccatca
     6961 tagtgagcat caaggcggta agccagtcgc gacgcgccaa ctgaagcaga cgcacgcgtc
     7021 ccagccaggc aatgggcagc gcaattaacc ctaacgccag ataacgcccc atcgactgca
     7081 acatcgccgg gtattccggc acgatcaacg gcccgacaaa aataagcccc cacatcaacc
     7141 ctgctaacag ggcgtacagc acgccgctaa tcattactgg catccattgg tctgtcagaa
     7201 gaaagttcag gaagcagaca tgattgctcc cggcatcccg tcattatcag agaggatgcg
     7261 ggaggatgcc actttaatta actagttatt aacattaaaa agatggctta cgctttcggc
     7321 taacgttgtc gttgggtggc caatcagttt gctaagcgtt ttgctgtcat caaacagacc
     7381 gcctttcgat gcgccaacgt cagaatccgc cagcatatcc gccagtccgt cgggcagtcc
     7441 gacgcttttc agtgccgcgg cgaaatcggc ttcgctcaga ttttgatagg taacctgttt
     7501 gccgctctgt ttggtcagct ctgccgctaa ctgtgtcaac gtccaggcac tatcgcccgc
     7561 cagttcgtaa accttgcctt cgtgaccggc ttcgctaatc acgcgtgccg cagctgccgc
     7621 ataatctgcc cgcgttgctg aggcgatttt gccatcgccc gccgcaccga taaatacgcc
     7681 gtgttccagt gctgccgggg cgctggcgag gtagttttcg ctgtaccagc cgttgcgcag
     7741 tagggtgtaa acgatgccag aatcagccag cattttctcc gtctcgatgt gctcatcggc
     7801 gaggccgagc ggggaggtat ctgcatgtag caggctggta taagcgataa atttcacgcc
     7861 agccgccttt gcggcattaa taacattacg atgctgcggg gcacgttgac ccacttcgct
     7921 ggaagagatc agcagtagtt tttccactcc ctgaagtgca gatgtcagtg cggcttcatc
     7981 gccgtagtca gcctgacgca cggtaatgcc ttgtgctgtc agtgcctggg cttttgccgg
     8041 attacgaacg atagccacta tttggctggc aggaaccgtt ttcatcaagg attcaataac
     8101 atactgacca agttggccag tggcaccagt aatagcgatc atgggaagtc tccatcgttt
     8161 tgtcttgtgt ttaaatacgc taacacctaa acttactttt agtaagtacg tacaaaaagg
     8221 taagtatgaa atgagtcagg ttagcctgtc gcaacaactg aaagagggca atctctttgc
     8281 ggaacagtgc ccgtcgcgcg aggtgttgaa acacgtcacc agccgttggg gggtgttgat
     8341 tctggtggcg ctacgcgaag gtactcatcg ctttagcgac ctgcggcgca aaatgggcgg
     8401 ggtgagtgaa aagatgcttg cgcagtcgtt acaggcgcta gagcaggatg gttttcttaa
     8461 ccgtatcgcg tatccggtgg tgccgccgca tgttgaatac agcctcacgc cactgggcga
     8521 gcaggtgagt gaaaaggttg ctgcactggc ggactggatt gagttgaatt tgcccgaggt
     8581 gttggcggtg cgggatgaac gtgcggcata actgccggct gcgctgcgct tatccggtcg
     8641 acggttcatg gcttgtaggg cggataagat gcgccagcat cgcatccgcc aataaatgcc
     8701 ggatgcggtt gatgatttat aggccgaaaa agaagtgttt tacttactca aatccacctg
     8761 ataaatcgca aacccgatat catcggtcgc gactttattc atcggatact gccctttctc
     8821 tttaataaac gctgcggctt tatctgacgg ggaagtttcg aaacggatat ccagtttctt
     8881 atcggcagct atcggtgcta aacgccagtt gttatctgcc gccgggtgaa tttcccccgc
     8941 acgtttcgac tcatcagcaa tccacgctgc cagcaccgag cggttctcat ccggtgaagc
     9001 aaaagcgata tggctgtcgc ccgtcccggc aaatttgccg ccgtaagcgc gatagttatt
     9061 ggtggcaacg aggaacatag cgttcggatc aatcggcttg ccattaaagg tcaggttctt
     9121 aatcctttcc gcattggcat taatcatctg gcactcgccg tcataacggg cgggctgggt
     9181 aacatcaatc tgataattca caccatcaat aacatcaaag ttataagtgc ggaaaccatc
     9241 ccagttgatg agtgactgtg gtttcgtgct gttgggatca atctggttaa actgtccggc
     9301 ggagcactcc agccactctt tcacctcttt accgctggct ttcaccacaa tcagcgtatt
     9361 gggatagaga taaagatcgg cggcattacg gaaggtcaac tggccttttt ccacctccac
     9421 atagcttgcc gggtcatttt tgcgaccacc gactttaaac ggtgcggcag ctgaaagcac
     9481 cggcagtttt gccagatccg gatcgccctg aatgtaatgc tcgacatacg ctttttgcgc
     9541 gttgttcacc acctgcacgg tcggatcgtc ctgcaccagc gccagatagc tatacatatt
     9601 gtcggcagat ttaccgattg gcttgctgac gaactggcgt gtggcatcgt gatcggcttt
     9661 gagtgtttct accagcttgc tgtcttccgc tgcgagtgat tttttattag cgatatcgta
     9721 aatcggccgt gcttccgctt tcgcctgcgt cacctgccat ttaccgctgt cattactgag
     9781 ttgtaagtcg accaccccaa gatgatcgcc ccacatgcct ggcattaccg ccggaacacc
     9841 attcagcgtg cctttggcga tatcagcccc ttcgatatca gcaaaatctt tacccgggaa
     9901 aacggcgtga gcatggccaa acataatggc gttaacgccc ggaatttcac tgaggtaata
     9961 aactgagttt tccgccatca ctttatacgg atcggcagac agcccggaat gtgccagaac
    10021 gacaacaaca tcggcacctt tctcgcgcat ttcaggcacg tatttgcgca cggtttcggt
    10081 aatatcattc accgtcactt tcccggataa attagcttta tcccagccca tgatttgtgg
    10141 cggcacgacg ccaatatagc caatcttcag cgtctgtttt tttccgtctt tatcgaccac
    10201 ttcggtatct ttaattaaat acggtgtaaa cattggctgt ttggttctgg cgtcaatgac
    10261 gttggcattt acataaggga atttcgctcc cgccagcgca tttttcaggt aatccagacc
    10321 gtagttaaat tcatgattgc cgagtgtacc gaccgtataa tccagcgtat tcagcgcctt
    10381 ataaaccgga tgaacatcac ctgctgttaa tcctttcgcc gacatgtaat cggccagcgg
    10441 actcccctga atcaaatcgc cgttatcgac cagtacgctg tttttcactt cattgcgggc
    10501 atcgttaatc aggcttgccg tacgtaccag tccgaatttt tccgtggcgg tgtctttgta
    10561 ataatcgaaa tccatcatgt tgctatgcag atcagtggtt tccatgatac gcagatcgac
    10621 cgtcgctgca ttcacactgg cggcaatcag cgtggccagg agcgttgcgc taaacttaat
    10681 catcagggac atccttttat catcgggaat acgaaagaaa agggagaata aacgtcttac
    10741 ttatagaaca gtgaagaatg ccacaatttt acgctttgaa aatgatgaca ctatcacagt
    10801 tggcgcattc attaacgata gggtataagt aaaacaataa gttaacaccg ctcacagaga
    10861 cgaggtggag aaatgttaga tcaagtatgc cagcttgcac ggaatgcagg cgatgccatt
    10921 atgcaggtct acgacgggac gaaaccgatg gacgtcgtca gcaaagcgga caattccccg
    10981 gtaacggcag cggatattgc cgcacacacc gttatcatgg acggtttacg tacgctgaca
    11041 ccggatattc cggtcctttc tgaagaagat cctcccggtt gggaagtccg tcagcactgg
    11101 cagcgttact ggctggtaga cccactggat ggtactaaag agtttattaa acgtaatggc
    11161 gaattcaccg ttaacattgc gctcattgac catggcaaac cgattttagg cgtggtgtat
    11221 gcgccggtaa tgaacgtaat gtacagcgcg gcagaaggca aagcgtggaa agaagagtgc
    11281 ggtgtgcgca agcagattca ggtccgcgat gcgcgcccgc cgctggtggt gatcagccgt
    11341 tcccatgcgg atgcggagct gaaagagtat ctgcaacagc ttggcgaaca tcagaccacg
    11401 tccatcggct cttcgctgaa attctgcctg gtggcggaag gacaggcgca gctgtacccg
    11461 cgcttcggac caacgaatat ttgggacacc gccgctggac atgctgtggc tgcagctgcc
    11521 ggagcgcacg ttcacgactg gcagggtaaa ccgctggatt acactccgcg cgagtcgttc
    11581 ctgaatccgg ggttcagagt gtctatttac taaattcaga tggcagaaac agtgtatttc
    11641 ctgattctgc catcctgatt tcttccaacc taaaa
//
LOCUS       DAPGOF010000158        11488 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.159, whole genome shotgun sequence.
ACCESSION   DAPGOF010000158 DAPGOF010000000
VERSION     DAPGOF010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.159"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            94..1707
                     /gene="mppA"
                     /locus_tag="RHV13_003730"
     CDS             94..1707
                     /gene="mppA"
                     /locus_tag="RHV13_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HDV1243216.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(1758..2789)
                     /gene="ynaI"
                     /locus_tag="RHV13_003731"
     CDS             complement(1758..2789)
                     /gene="ynaI"
                     /locus_tag="RHV13_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HDV1243217.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            3033..3290
                     /locus_tag="RHV13_003732"
     CDS             3033..3290
                     /locus_tag="RHV13_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HDV1243218.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(3340..4290)
                     /gene="uspE"
                     /locus_tag="RHV13_003733"
     CDS             complement(3340..4290)
                     /gene="uspE"
                     /locus_tag="RHV13_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HDV1243219.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(4442..5194)
                     /gene="fnr"
                     /locus_tag="RHV13_003734"
     CDS             complement(4442..5194)
                     /gene="fnr"
                     /locus_tag="RHV13_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HDV1243220.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(5389..5904)
                     /gene="ogt"
                     /locus_tag="RHV13_003735"
     CDS             complement(5389..5904)
                     /gene="ogt"
                     /locus_tag="RHV13_003735"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDV1243221.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(5915..7441)
                     /gene="abgT"
                     /locus_tag="RHV13_003736"
     CDS             complement(5915..7441)
                     /gene="abgT"
                     /locus_tag="RHV13_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HDV1243222.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFIGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            complement(7478..8923)
                     /gene="abgB"
                     /locus_tag="RHV13_003737"
     CDS             complement(7478..8923)
                     /gene="abgB"
                     /locus_tag="RHV13_003737"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HDV1243223.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESASFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWCFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            complement(8923..10233)
                     /gene="abgA"
                     /locus_tag="RHV13_003738"
     CDS             complement(8923..10233)
                     /gene="abgA"
                     /locus_tag="RHV13_003738"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HDV1243224.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARHQGALAQWIAAFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWSRGI"
     gene            10409..11245
                     /locus_tag="RHV13_003739"
     CDS             10409..11245
                     /locus_tag="RHV13_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV1243225.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELNFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVREIFAESGLLFDS
                     AA"
BASE COUNT         3020 a         2805 c         2825 g         2838 t
ORIGIN      
        1 cctgtgtcac ttttttgatg gaagttgttt gcatttcttt aaggcgaaac aaataattac
       61 gcatcaattt taatgtcggt tagagggaaa cttatgaagc actctgtttc agtcacatgt
      121 tgtgcgctgt tggtcagcag catttctctt tcgtatgctg cagaagttcc gagcggcaca
      181 gtactggcag agaagcagga gctggtgcgc cacattaaag atgagcctgc gtcgctggat
      241 cccgctaaag ccgtgggcct gccagagatt caggtcattc gcgatctgtt tgaaggactg
      301 gtgaatcaga acgagaaagg ggagattgtc cccggcgttg cgactcagtg gaaaagtaat
      361 gacaaccgta tctggacttt taccctgcgc gataacgcaa aatgggcgga tggcacaccg
      421 gtaacggcgc aagattttgt ctacagctgg caacgtctgg tggacccaaa aacattgtcg
      481 ccatttgcat ggtttgccgc gctggcggga atcaacaacg cacaggcgat tattgatggt
      541 aaagctacgc ctgaccagct tggcgtcacc gcagttgatg cccatacttt gaaaattcag
      601 cttgataaac cgttgccgtg gtttgtgaat ttaaccgcta actttgcctt cttcccggtg
      661 caaaaagcca acgtagaaag cggtaaagag tggacgaaac ccggaaatct gatcggcaat
      721 ggcgcttatg ttcttaaaga gcgcgtagtc aatgaaaaac tggtcgtggt accgaatacc
      781 cattattggg ataacgccaa aacggtactg caaaaagtga ccttcctgcc aattaatcag
      841 gaatccgcag ccactaagcg ttacctcgcg ggggatattg atatcaccga atccttcccg
      901 aaaaatatgt atcagaagct gttgaaggat attccggggc aggtttatac gccgccgcag
      961 ctcgggacct attattatgc gtttaacacg caaaaagggc cgacggcaga tcagcgcgtt
     1021 cgtctggcat taagtatgac gatagatcgc cgcctgatga ccgaaaaagt attagggacg
     1081 ggcgaaaagc cagcgtggca ttttacacca gatgttaccg ccggatttac gccggaacct
     1141 tcgccgtttg aacaaatgag tcaggaagaa ctgaatgcgc aggcaaaaac tttgttgagc
     1201 gcagctggtt atggtccgca aaaaccgttg aagctgacgc ttttgtataa cacttcagaa
     1261 aaccatcaaa aaattgcgat tgctgtagca tcgatgtgga aaaagaacct tggcgtagat
     1321 gttaaattgc aaaatcagga atggaaaacc tatatcgata gccgtaacac cggcaatttt
     1381 gatgttatcc gcgcctcatg ggtgggggat tataatgaac cctccacttt cctgacatta
     1441 ttaacgtcaa cgcattcagg aaatatttca cgctttaaca atccggcata cgacaaagtt
     1501 ctggcccagg catcgacgga aaataccgtt aaagcgcgta atgccgatta caacgcggca
     1561 gaaaaaatcc tcatggagca agcaccgatt gcaccaattt atcaatatac caatggacga
     1621 ttaatcaaac cgtggctgaa aggttatccc attaataacc ctgaagatgt ggcgtacagt
     1681 cggactatgt atattgtgaa gcattgatgt gatgggaact ggcgttaccc ttgtggataa
     1741 cgccagtgat gtctgattta gcgaccctgt tccggcggtg taatgttatc catatacagt
     1801 gtctggctgg ggaaggcaaa gtccgcgccg tgtgactgca caatatcgat aatcttcaaa
     1861 taaacgtctt gctgtgcagc aagccactca gcccataccg tggttttggt aaagcaataa
     1921 accataatat tcaatgacga gtcagcaaac tggttgaaat aaaccagtaa ggtttgtcgc
     1981 tggtcgatgg ccgggtgatt tttcagcatc tcacgtacgg cttcgacaat aacgcccact
     2041 tttgccgcat cctcataacg taaaccaatg gtcgtggtaa tgcggcggtt ggtcattcgt
     2101 cctgggtttt ctacgctgat cgacgaaaac agcgagttcg gtacgtacaa tggacgatta
     2161 tcaaaggtcg taattttggt aattcgccag ccaatttccg ctactgtacc ttcgatattt
     2221 ctgtccggtg aacggatcca gtcgccaata ctgaaaggac ggtcgaaata gagcatgatc
     2281 ccggaaaaga aattactcag aatatcttta ccggccatac cgacagccag accaccaata
     2341 ccaccaaagg tcagcaagcc agaaaggctc atgccgaaat gttcgccata aagcagaaca
     2401 agcaccacaa taatggtgat tttgataatg cgcgacataa tccgcgcact ggtgatatcg
     2461 cgaccttttt taatctgctg tttttcaaac tgattaatca gcaaaaatag cttaatcgtc
     2521 agaataaccg caatcaggga cgtacagata aaatcgataa cgcctggggt gataaatttg
     2581 agtttatagt tttctataac ataattaata atgctaccaa cagcactgat aattatggtg
     2641 tagattaaaa attgcaccgc atggaataaa aaaccttttc ttttacgatt tccgcggcga
     2701 aaccaaaagc tcatcagaat caatgctgcg cagctaccga aaataatgac cagattaagc
     2761 gcattatttg taaacagttc agcgatcatt gttttatcag gctcctccag ataattgtcg
     2821 tcatgccgga aacccctggc ggggctattt taccgcgaca attcattcag atcatcaata
     2881 gtcagggaag gaagtagcaa cattagctaa ctaatcaatg cgttgattgt aaatccagct
     2941 aagaggtgag gttttcagag cagacaacgg tgaaatgtca tggtattgtt acgtttaggt
     3001 aacaagaaat ttgtctgcac aaggattaca tcatgattat ggcgaaactg aagtcagcga
     3061 aagggaagaa atttctcttt ggtttgttgg cggttttcat tattgcggcg tcggttgtga
     3121 ctcgcgcgac tatcggcggc gttatagaac agtacaatat tccgctgtct gagtggacga
     3181 catcaatgta tgtgattcag tcatcgatga tttttgttta tagcctggtc tttactgtgt
     3241 tgctggcaat cccgttggga atttatttcc ttggcggcga agagcagtaa gtaaaaaata
     3301 ggcccgataa ctcgggcctt gtcagttatt gaagagtcgt taatcgtctt cttcgtcatc
     3361 cagttcaacg ggtgtctgat actggtcagg tttaatgacc aacagatcgc agcgaagatg
     3421 atcaatcacc tgttccgccg tgttgccgag gaatgctgct gaaataccgg tgcgtcctac
     3481 cgtgcccaga accacaatcc ccgcctgtaa gtgctccgcc aaatcaggaa tcacctcttc
     3541 tggcagacct ttttctacgt gcgtcatgtt ttcattaatg ccgaatttct gccgcagagc
     3601 tttcattgcc agcaaatgtt gcccacgaat ggcatcgtta taaacgctcg gatcaaattc
     3661 cggcagttca atcgcgatat taattggcgt tactgggtaa gcgccaacca gatgaacttc
     3721 ggtatggttg acttgttctg ccagttcgat cgtctctttg accagttttt cattgagcgc
     3781 attatgatac ggctcttcac tggcgagatt caccgccacc agcgccttac ctccttccgg
     3841 ccacggctgg tctttcacca tccacaccgg gcttgggcat ttgcgtaaca gatgccagtc
     3901 cgttggcgta aaaatcaccg cttccagacg gtcatgttgg tgcgccattt ttagcaccaa
     3961 atcgtgcccg ccgctgatca cttcctgaat gatggcttcg aaaggacggt tatgccagac
     4021 cactttaatt tcaatgggaa cgccagcatt gagataatat tttgcctgct cgtggatcca
     4081 ggctgtacgc tggctgatga cgccctgacg catagcggta cgttcgtccg gggagagcag
     4141 ggtggtcatt tcgtatgaga agtcatagat cggcaaaaag gctttaattt tgccaccaat
     4201 ccgttgatgt aaataaacag ctcgccgcaa tgctggttgg tcgtcctggt taggatcgat
     4261 aacaacgagc atgttctgat acatagccat acagggtctc cttacaacaa ctgtcaacgc
     4321 agtttgtaat taaaagatta acccatatct ggtgaatgaa acagtgatga accttctgcc
     4381 agatcaataa atcagaaaaa tttaatgata tgacagaagg atagtgagtt atgcggaaaa
     4441 atcaggcaac gttacgcgta tgaccagcaa gctgggccag cgcatcgtta ttttcgatgg
     4501 tgatgtattt acctttgact gccagcatgc cgcttttctg gaagcgaccc agcagacggc
     4561 tgatggtttc taccgtcagg cccagatagt taccgatatc gccacgagtc atcgtcaggc
     4621 ggaattcacg aggggagaag ccgcgttggg caaaacgacg ggacaggttg tagatgaatg
     4681 cagccagacg ttcctcggca tttttcttcg acaacagcag gatcatgtcc tgatcgcctt
     4741 tgatttcacc gctcatcaga cgcatcatct gctgacgcag attcggcatt ttaccggaca
     4801 aatcgtccag cgtttcgaac gggatttcac ataccatcga ggtttccagc gcctgcgcga
     4861 agctcgggtg atggccgctg ccgatggcgt caaatcccac caggtcgcct gctaaatgga
     4921 aaccagtgat ttgctcgtcg ccttgctcag tgatggtata acttttaatc gtaccggagc
     4981 ggatggcata aagcgattta agttcatcac cagccttaaa cagcgtctgg cctttctgaa
     5041 taggcttctt ccgctcaatg atattatcaa gctgatcaag ctcatgttcg ttgagtgtga
     5101 acgggatgca aagctggctg atgctgcaat cctggcaatg gatagcacaa ccgccagact
     5161 gaatgcgccg tataattcgc ttttccggga tcataggtct gctcaagccg taattgatat
     5221 ttgtcaattt taacatcttt ttagggagca agtaagtcta agcaaacctt aacagcagag
     5281 aattccgata ttagatgtaa atatatgtct atctatttga aaacccttaa gttgttaagg
     5341 gtaactttac ataaaagtgt gaacaagctg gcacaaattg tttaatgttt acagcaaaag
     5401 ataaccttcg tggcgcaata accactcttt tcgctgaact ccgcctgcat atccggtcat
     5461 ggtgccgttt cggccaataa cccgatggca aggtacgacg atgctgatgg gattcgatcc
     5521 gtttgccgca ccaacggcac gcgccgcgcc agggcggccc aattgttcag ccagttggcc
     5581 gtaatgcatt acctgcccgc aagggatagt gcgtagtgtt ttccagactt cgcgctgaaa
     5641 tggcgtcccc cctgtggcag tgggaagcgt atcaataatg ctaagattac cggcaaaata
     5701 atcacgaagc ttgtcgctta aaccgcctgg gttggtggca gaaatgcgct catagccttc
     5761 tttgcgataa tggatgtcca gcagctgcac catgcgttcg ctgtactctt cccattcaac
     5821 cgcccgcagg cgaaattgct catcgcaaat cacccacagt ggacccagtg gtgtggcaat
     5881 tttttcttca agtaatctca gcatccgttc tctcttaaga caaacgtggg taaatacccg
     5941 gacctatcgg caggcccaca agataccacg ccagcaacat cagcagccat accaccaaaa
     6001 agataagcgg atagggcaag actaacgaat agtaagtacc cagtttcgcg tctggtttgt
     6061 agcgttgcag gaatccaaga aacagtggaa caaaaggaga tactggcgct aaaggcaata
     6121 cggatgagtc ggcaatacga aagaggattt gcgcaaatgc cgggtgaaag ccaagtagca
     6181 taaacattgg tacgaaaatg ggggccagaa tcgaccagat tgcggaaccg ctggcgataa
     6241 acatgcataa gaaagaggaa agcaacgcca gaccgataaa cgccgggatg ccgctaagcc
     6301 ctgaactttc cagtatatcg gtcagcccca cggcgatgaa tttccccatg ttgctccagt
     6361 taaacatggc gacaaactgg gcgaggggaa aaaccatcac gataaatccc gccatctctt
     6421 tcatcggttc aatcattaaa tgcggtaaat ccgcctgacg tcgaattgtg cgggtagcga
     6481 tgccataagc cagcgagaca acaaagaaaa aaagaatgat cagtggcacg atacctttaa
     6541 taaagggtga tggcatcacg gtgtgattaa tcggatcgcg caatatcccg ttttccggga
     6601 tcaccatcag cgcaatcgca gcaataaaaa gtagcgatac gacacctgct atgcgtaaac
     6661 caaaacgctg actttcggtc aatgtctgca gtttctcatc gctgtttccc tgccattgac
     6721 ctaaccgtgg ctcgatgatt ttgtcggtta tcaggccgcc aacaatcgtc agtacgacta
     6781 cggagctggc cataaaatac cagttatcaa ttacactgac gtgcatttgc ggattgaacg
     6841 cagctgccgc ttccgtgctg atccccgaca gcaacacgtc ggttgtgaca atcagtaaat
     6901 tagccgtaaa gccgcaacct acgcctgcaa tggcagccag taaacctgca actggatgcc
     6961 tgcccaccgc cagaaaaatc agcgcaccca tcggtggcat gatcactaac gccgcatcgg
     7021 aagaaatgtg gctgaaaaaa gcaataaaca gcaccatata actggcgtag cgggcattaa
     7081 catgcgatgc cattttaacc attagtgctg gcagtaagcc gacgcgctcc gccagaccgg
     7141 cacctaaaac cagcgccagg atcgcaccaa gtggagcaaa accgctaaag tttttaataa
     7201 cattgggtaa aaaccagtgt aatccttcca cactgagcag gtttttcacc accaccggcg
     7261 taccatcggt cgggtttttc gcactgacgc caaaggctga caaaattgcc gtcgtcacca
     7321 tgagtacgat tatcaaatag ataaagagca aaaaaggatg gggaaccttg ttaccaattc
     7381 tttcgaccca gccatagagc ttcccggatt gggaggacga cggtatggat gacatactca
     7441 tgggcattcc tcggttgttg tgtttgcgtt gttgttgtta ttttaaaggt gacggtgtca
     7501 cgtttttcgg gatagggcag tgatacggtt gcgtgtccgt aacttgctga tgctcttgtt
     7561 ggcattcttg caatagccct gaatcaatga agagattgac tgtggttgct gccatagttt
     7621 tcgccgccag cagcattcct ttatgagcaa tagatgttcg cccctggcta accagttgcc
     7681 acgtatgtag cggtgtaccg acggcaaaac aggggctgaa acactgggca acaggcagtt
     7741 tccaactgac gtcgccgaca tcagtcgatg ccgcaagcac gttatcggtg gcggcatatg
     7801 gagcgacctc attcgccagt accgtttcac gatgacgtag tgcaaaagcc ttgccgtttt
     7861 cgccaccggt tgcggcgata ttattcagac tgttttgccg atcgttgggg gtgagcgtag
     7921 cctgaatttg tttcgcaaaa gccagttctt cggagttcca ttccggggta ccaaaatggg
     7981 atagggcatg gtacatggca ttttctaagg tgcgattcgg gagataactg gaacaggctt
     8041 tgtcgaagcg gcattcaacc gtggtttcgg tcatcaatgc cgcaccttcg gcgattttgg
     8101 cgacccgatc ataaatatgc tgcacgtcgg tcatttcggg ggcgcggata agataaagca
     8161 cttctgcctg cgcctggacc acgttgggcg agatcccgcc gctatttgtg atggcatagt
     8221 gtacgcgcgc tttttcaata atatgttcgt tgaggaagtt ggtgccagtg gtcatcaacg
     8281 ttacggcatc aagggcgctg cgtcccaaat gaggggaatt cgcggcatgt gctgcgatcc
     8341 ctttaaagca ccatgatgcc tgaatgtttg ccagcgtgcg ggtattgaac atgccggcaa
     8401 aggcttccgg gtgccaggtg agtgccgcat ccacatcatc aaataccccc tcgcggacca
     8461 tgaacgtttt acccgagccg ccttcttcgc caggacaacc ataaaagcgc actgtgccgc
     8521 cttgcccata ttgttccagc catttcttga cggctattgc agcggcaaag gcggcggttc
     8581 ccagcaaatt gtgtccgcaa ccgtgaccat tttcaccggg cgtcgcggat gtaggttgcg
     8641 cgcaaccagc ttgctgactt aaacctgcca gggcgtcata ttctcccagc agggcgataa
     8701 ccggtttgcc ttgaccaaac gaagcaataa atgcatttgg gatattgcct acgttgcggg
     8761 taacggtgaa gcttgcagat tccagcgccg aagccagatg ctccgctgac cagaactctt
     8821 caaaacgtgt ttctggatga tcccagattt gatcggcaat atcggtataa cgttggcgat
     8881 cggcttcaat cgcatcgtcg ataaaacgat agatttcctg catcagatac ctcgcgacca
     8941 gggaaaattg agcgcggtgc gcgccagcgt ttcgacggca atagcgagaa cctgctcgtc
     9001 aaaatcgaat ttttcgttgt gatgacctgc cgccagctgt gtgccaaaca ccacgtagga
     9061 ggcttgccct tgatgttgct gcacgcgggc catcattaat gtggcatctt cggaacccgc
     9121 aggcgcttca acacgttcaa tggcctgatt gacccccgcg acctgagccg cctgactttg
     9181 caaccatgcg acccattgcg gcgaaggaga actggcggta gctgcaccca tcagacgagt
     9241 ttcaacgccg acaccataca tggttgctgc gccctgaatc gcttgttgtg cacggtcaaa
     9301 aacatattga ttaatgacgt cgctggcccc gcgtgtttcc actttcagca acgccgaggc
     9361 aggaacaacg ttacggccgc ttcctgcctg cataacgccc acgtttactc tggaagctcc
     9421 ttcgctgtgc ggggcgattg catgcagtgc aagagttgct tgtgctgccg ccaacaaggc
     9481 attgtgaccg tcttctggtt ttgcgcctgc gtgagcggcg gtcccggtga agtgcgcgtc
     9541 aaatttggtg gttgccataa aattatcact gccgcacacc acggtgcccg caggtacgcc
     9601 agtgccaatg tgcacggcag taaaataatc aacatcatct acgacacctg catcgaccat
     9661 cgcccgcgcg ccacgcgtac cttcctctgc aggctgaaaa atcagtttga tgacgccatg
     9721 tagtccggac tcgaattgtt taagggtatg cgccagccca agcccaatgg cggtatgtcc
     9781 atcatgacca caggcatgca tcattccggc gttacatgac gcaaaaccgt cgcggtaggg
     9841 gcgatggctg acatcctgct cttcactgag atccagcgcg tccatatcga cacggaaagc
     9901 catcaccgga ccgggtcgac cggtatccag ggtggcgacg atacccgtga aaccaccttc
     9961 aaaagccgca atccattgtg ctagcgcacc ctggtgacga gcgcgctcga attcgcgttg
    10021 tagagtgagt tcatcaggta gtcccatccg gctactttca ttaactactt cgcggcccag
    10081 cgctagtgaa tagccgagct gctgcaattc ttccgcaaca agggtggcag tgcggaattc
    10141 cacccagcca gactctgcat agtgatgaaa atcacgtcgc cagtgcgata attttggggc
    10201 aagcgaatta acaaattgat tcaaagactc cataaccttt cccgtcatca gtaaaaagtg
    10261 tgacccggtt cacgtagcga tagtttttac ttatcactaa ctgatttttc acagttttaa
    10321 ccgttcataa attaccctga cacaatcatc tgcattaaag tagatgccag tttctttggt
    10381 ctgataaata acggttatcg gtggcgtcat ggcttttcag gtaaaaattc atcaaattcg
    10441 ggcttttgtt gaagtggctc gtcagggcag cattcgcgga gcgagccgaa tgttgaatat
    10501 gtcgcaaccg gcactgagta aatctattca ggagctggaa gaagggttag cggcgcaact
    10561 cttttttcgc cgtagtaaag gcgtgacgtt aactgatgcc ggtgaaagtt tttatcagca
    10621 cgccagtctt attcttgaag agctgcgcgc agcccaagag gatattcgcc aacgacaagg
    10681 gcaactggca gggcagatta atatcggcat gggggccagt atttcccgca gtctgatgcc
    10741 agctgtcata tctcgttttc atcagcagca tccgcaggta aaagtacgca ttatggaagg
    10801 gcaactggtg tcgatgatta atgaattgcg tcagggagaa ttgaatttca ccatcaatac
    10861 ctattatcag ggaccgtacg accacgaatt tacttttgag aaattactgg aaaagcaatt
    10921 cgcgatcttt tgccgcccgg gacaccccgc cattggtgcc cgttcgatca aacagttact
    10981 ggattacagc tggacaatgc cgacgccaca cggcagctac tacaaacagt tgagtgaatt
    11041 gcttgacgat caggcgcaaa cgccacaggt cggtgtagtc tgcgagacgt tctcagcctg
    11101 tatcagtctg gtggcaaaaa gcgattttct cagcatactg cctgaagaaa tgggctgcga
    11161 tcccttgcac ggacaggggc tggtgatgtt gccggttaga gaaatttttg ccgaaagcgg
    11221 cctattattt gattcagcgg cgtgatagtc gccagacacc actgaccgcg tcattaatca
    11281 cgcaattccg gcgagaatgc ggctatctgc aaagttaaaa ctgcataaaa aaatagagtc
    11341 tgtcgacatc cgccagactc tacagtacac acagcagtgc atccgcgtct taaatccggc
    11401 attgtctcct ctgcgccggt gactgtgtta taacggtttt aacctttagt tgccaatttt
    11461 cttcagtgac agatttcacg aaaatata
//
LOCUS       DAPGOF010000159        11270 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.160, whole genome shotgun sequence.
ACCESSION   DAPGOF010000159 DAPGOF010000000
VERSION     DAPGOF010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.160"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(138..1802)
                     /gene="glnS"
                     /locus_tag="RHV13_003740"
     CDS             complement(138..1802)
                     /gene="glnS"
                     /locus_tag="RHV13_003740"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HDV1243226.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRTGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(2005..3951)
                     /gene="nagE"
                     /locus_tag="RHV13_003741"
     CDS             complement(2005..3951)
                     /gene="nagE"
                     /locus_tag="RHV13_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HDV1243227.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4284..5084
                     /gene="nagB"
                     /locus_tag="RHV13_003742"
     CDS             4284..5084
                     /gene="nagB"
                     /locus_tag="RHV13_003742"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDV1243228.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5144..6292
                     /gene="nagA"
                     /locus_tag="RHV13_003743"
     CDS             5144..6292
                     /gene="nagA"
                     /locus_tag="RHV13_003743"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDV1243229.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6301..7521
                     /gene="nagC"
                     /locus_tag="RHV13_003744"
                     /gene_synonym="nagR"
     CDS             6301..7521
                     /gene="nagC"
                     /locus_tag="RHV13_003744"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HDV1243230.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7569..8321
                     /gene="nagD"
                     /locus_tag="RHV13_003745"
     CDS             7569..8321
                     /gene="nagD"
                     /locus_tag="RHV13_003745"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HDV1243231.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            8718..10382
                     /gene="asnB"
                     /locus_tag="RHV13_003746"
     CDS             8718..10382
                     /gene="asnB"
                     /locus_tag="RHV13_003746"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HDV1243232.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFEAYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10762..10838
                     /locus_tag="RHV13_003747"
     tRNA            10762..10838
                     /locus_tag="RHV13_003747"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10796..10798,aa:Met,seq:cat)
     gene            10847..10931
                     /locus_tag="RHV13_003748"
     tRNA            10847..10931
                     /locus_tag="RHV13_003748"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10881..10883,aa:Leu,seq:tag)
     gene            10955..11029
                     /locus_tag="RHV13_003749"
     tRNA            10955..11029
                     /locus_tag="RHV13_003749"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10987..10989,aa:Gln,seq:ttg)
     gene            11064..11138
                     /locus_tag="RHV13_003750"
     tRNA            11064..11138
                     /locus_tag="RHV13_003750"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11096..11098,aa:Gln,seq:ttg)
     gene            11154..11230
                     /locus_tag="RHV13_003751"
     tRNA            11154..11230
                     /locus_tag="RHV13_003751"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11188..11190,aa:Met,seq:cat)
BASE COUNT         2810 a         2917 c         2845 g         2698 t
ORIGIN      
        1 acgcaaactc aatatattgc agagatcatg taagcctgat aagcgtagcg catcaggcaa
       61 tttagcgttt gaaactgttt gtctgttcct tgccggatgc gacgtaaacg ccccatccgg
      121 catagcgaaa cttaaaatta ctcgcctact ttcgcccagg tatcacgcag cccaacggtg
      181 cggttaaata ccggtttttc cgccgtagaa tggcggctat cgaggcagaa gtaaccttca
      241 cgctcaaact ggaatgcttt acccgcaacc gcatctttca gcgacggttc agcaaagccc
      301 tgtttgatca caagcgattc cgggttaatc accgacagga aatcatccgc agcacctggg
      361 ttaggcacgc tgaacagacg gtcatacaaa cggatttcaa ccggcagcgc atgtgccgcg
      421 ctcacccagt gaataacgcc tttcacttta cgaccatcag ccggatcttt gcttaaggtg
      481 tcggcgtcat aagtacagaa gatggtggtg atattacctt cggcatcttt ctccacgcgt
      541 tctgccttaa tcacataagc attacgcaga cgcacttctt tacccagcac cagacgtttg
      601 tactgcttgt tagcttcttc gcggaaatcg gcgcgatcga tccaaatctc accgctaaac
      661 ggcacctgac ggctgcccat ttccggtttg ttcggatggt tcggcatggt aaccatttcg
      721 ccttcgccct gatagttttc gataaccagt ttcaccggat cgataaccgc cattgcgcgc
      781 ggcgcatttt cgttgagatc ttcacggatg caggattcca gcgacgccat ctcaatggtg
      841 ttgtcctgct tggtcacgcc gatgcgtttg cagaactcac gaatagaagc cgcagtgtaa
      901 ccacgacgac gcagaccgga aatggtcggc atacgcgggt catcccagcc ttcaacgtgc
      961 ttgtcggtca ccagcaggtt cagcttacgc ttggacatca cagtgtattc cagattcagg
     1021 cgcgagaatt cgtactggcg cgggtgaaca ggaatggtga tgttgtccag tacccagtcg
     1081 tacagacgac ggttatcctg gaactcaagc gtacacagag agtgcgtaat accttccaga
     1141 gcatcgctga tgcagtgggt gaagtcgtac atcgggtaga tgcaccactt gttgccagtc
     1201 tggtggtgtt cagcaaattt aatgcggtac agcaccggat cgcgcatcac gataaacggt
     1261 gaagccatgt cgattttcgc acgcaggcag gctttacctt cttcaaaacc gccggtacgc
     1321 attttctcga acagcgccag gttctcttca acgctacggt cgcggtacgg gctgttttta
     1381 ccaggctgcg tcagggtgcc gcggtattcg cggatctgtt ccggcgtcag ttcatcaacg
     1441 tacgccaggc ctttattgat cagttcgatc gcataggcgt ggagctgatc aaaataatcg
     1501 gaggagtaac ggacgttacc agaccagtga aaacctaacc actctacgtc gtttttgatc
     1561 gactcaacat actcgatatc ttcttttacc gggttagtgt cgtcgaaacg caggttgcac
     1621 tggcctttat agtcctgggc gatcccgaag ttcaggcaga tagatttcgc atggccaata
     1681 tgcagatagc cattcggctc cggcgggaaa cgggtgtgta ctgtggtgtg cttaccactg
     1741 gccagatctt catcgatgat ctgacggata aagttagtcg ggcgggcttc tgcctcactc
     1801 atcgtggatt cctcaaagcg taaacaacgt ataacggcgt atgatcttat aagcgggaca
     1861 ggctgacaac tgttagtttt ttaaaattga taatctaaca aggattatgg ggatgattga
     1921 tgaaaaaaaa agcggcagag aagatctcca ccgcttgtca ttgttggatg cgacgctcaa
     1981 gcgtcgcatc aggcataaag cagattactt tttgatttca tacagcggcg tttgacccgc
     2041 cacaacatgc ccctgagctt taatgataag gccgctgaaa tcgtcgatat tgctgcaaac
     2101 caccgggcta atcatcgaac gggcgttagc gttcaggtaa tccagatcca tttccagaat
     2161 cggttgtcct gcgcttacct gcgcgccctc ttccactaga cgtttaaagc ctttaccttc
     2221 cagcgctacg gtgtcgatac ccatatggac gacgatctcc gcgccttttt cggtttccag
     2281 acagaacgcg tggttggtgt tgaagatttt cacgattgtc cctgcggctg gtgatacgac
     2341 gattttatct gtcggtttca ccgccacacc gtcacccacc gctttgctgg cgaatgcttc
     2401 gtcaggaacc tgatccagtg ccacgacatc accggtaatc ggcgatacca gctccgcgat
     2461 agataccgcg tttggtacag cctgcggttt tgctacagga gcggcagttg ccggagttgc
     2521 ttcagcagag gcagctgcta ccggaccgcg ggcaacgact ttcttcatcg catcgccgat
     2581 ggattctgct ttcgcgccaa caatcacctg aatagtctgt ttgttcagtt ttactacccc
     2641 agaagccccc agacgtttac acatcgtatc gttaacgcgg gcagagtcag ccactgtaag
     2701 gcgcagacgg gtgatacagg cgtcaattgc tttcaggttg tcagtgccgc caaccgcagc
     2761 aatatagttg gtcgccagtt gattgaggcc ttcttcagtg ttgctgttgg cttcttcagt
     2821 aacgatctcg tcttctttat cttcacgacc cggcgttttc aggttgaaca tgcggataac
     2881 caaactgaac accacgaagt agatagcgaa gaagacaaca cccataacca gcagcatcca
     2941 gacgttctgg ctggcggccg gcaggttata catcaacgcg tagtcgatag cccccgcaga
     3001 gaaagagaag ccagcatgga tacccagcag cgttgccaca aacaggctga taccggtcag
     3061 cagtgcgtgc aggaggtaca gcagcggtgc aaggaacatg aacaggaatt ccagcggctc
     3121 agtcacaccg gtcaggaacg cagtaacagc aacagaaagc agcatcccgc caaccatcgg
     3181 acgacgctct ttcggtgctg cgaagtacat cgccagcgcc gcccccggca gaccgaacat
     3241 catgatcggg aagaagccgg acatgaacat ccccgcggtg ccgtcaccgg catagaagcg
     3301 gttgatatca ccgtggaaaa ccgtacccgc cgcgttggtg aattcaccaa tctggaacca
     3361 ggcgatggtg ttcagcacct gatgcagacc ggttgggatc agcagacggt tgatgaaacc
     3421 aaagataccg gaacccagcg cgcccgcaga aacgatccac tcgccgcctg catggatagc
     3481 gtgctgtacc ggcggccaga cataaccaaa aatggccgcc agcaccagac agaagaagcc
     3541 ggtggcgatc ggcacaaagc gtttgccgcc gaagaagctc aggaagtccg gcagtttaat
     3601 atcggaccaa cggttatagg ctgcgccacc aaccagaccg gtaatgatac ccgccagtac
     3661 acccatgtta atttctgggt tgatggtcac catcgctttg gttaacacaa agtaacctac
     3721 cgcacccgcc agcgccgccg cacctgcgct gtctttcgac cagctggatg ccacaccgat
     3781 ggcgaagatt aatgcgaggt tatcaaaaat cgcaccgccc gcctgggcaa taaacgcaac
     3841 gttaagtaaa tctggctgac cgaatcgcag caacagtgcc gccaccggca gcaccgcgat
     3901 agggagctgt aacgccctac cgagtcgctg gaaaaaacct aaaatattca tcttattccc
     3961 cctacgagaa ccctatttgg ctcgtttaaa gccgtatttt tattttgctg caaattgtac
     4021 tgccgatgtt ctgtaatcag attgttagat catctgctac agagtgtgtg aaaatttaat
     4081 tcgtatcgca aattaaacgc gtgtcttttg tgagttttgt caccaaatat cgttatcatc
     4141 actccctttt actggctaaa ccagaaaact tattttatca ttcaaaaaat caggtcggat
     4201 tgacgcctgt ctgcgcaaat ccaggttacg cttaaagatg cctaatccgc caacggctta
     4261 cattttactt attgaggtga ataatgagac tgatccccct gactaccgct gaacaggtcg
     4321 gcaaatgggc tgctcgccat atcgtcaatc gtatcaatgc gttcaaaccg actgccgatc
     4381 gtccgtttgt actgggcctg ccgactggcg gcacgccgat gaccacctat aaagcgttag
     4441 tcgaaatgca taaagcaggc caggtcagct ttaagcacgt tgtcaccttc aacatggacg
     4501 aatatgtcgg tctgccgaaa gagcatccgg aaagctacta cagctttatg caccgtaatt
     4561 tcttcgatca cgttgatatt ccagcagaaa acatcaacct tctcaacggc aacgccccgg
     4621 atatcgacgc tgagtgccgc cagtatgaag aaaaaatccg ttcttacgga aaaattcatc
     4681 tgtttatggg cggtgtaggt aacgacggtc atattgcatt taacgaaccg gcgtcttctc
     4741 tggcttctcg tactcgtatc aaaaccctga ctcatgacac tcgcgtcgcg aactctcgtt
     4801 tctttgataa cgatgtcaat caggtgccaa aatatgccct gactgtcggt gttggtacat
     4861 tgctggatgc cgaagaagtg atgattctgg tgctgggtag ccagaaagca ctggcactgc
     4921 aggccgccgt tgaaggttgc gtaaaccaca tgtggaccat cagctgtctg caactgcatc
     4981 caaaagcgat catggtgtgc gatgaacctt ccaccatgga gctgaaagtt aagactttaa
     5041 gatatttcaa tgaattagaa gcagaaaata tcaaaggtct gtaattgtta tccctgccct
     5101 ggctccttgc tcagggcaat attttttaaa atcgggggtc agaatgtatg cattaaccca
     5161 gggccggatc tttaccggcc acgaatttct tgatgaccac gcggttgtta tcgctgatgg
     5221 cctgattaaa agcgtctgtc cggtagcgga actgccgcca gagatcgaac aacgttcact
     5281 gaacggggcc attctctccc ccggttttat cgatgtgcag ttaaacggct gcggcggcgt
     5341 acagtttaac gacaccgctg aagcggtcag cgtggaaacg ctggaaatca tgcagaaagc
     5401 caatgagaaa tcaggctgta ctaactatct gccgacgctt atcaccacca gcgatgagct
     5461 gatgaaacag ggcgtgcgcg ttatgcgcga gtacctggca aaacatccga atcaggcgtt
     5521 aggtctgcat ctggaaggtc cgtggctgaa tctggtaaaa aaaggcaccc ataatccgaa
     5581 ttttgtgcgc aaacctgatg ccgcgctggt cgatttcctg tgtgaaaacg ccgacgtcat
     5641 taccaaagtg accctggcac cggaaatggt tcctgcggaa gtcatcagca aactggcaaa
     5701 tgccgggatt gtggtttctg ccggtcactc caacgcgacg ttgaaagaag cgaaagccgg
     5761 tttccgcgcg gggattacct ttgccaccca tctgtacaac gcgatgccgt atattaccgg
     5821 tcgtgaaccg ggcctggcgg gcgcgatcct cgacgaagct gacatttatt gcggtattat
     5881 cgctgatggc ctgcatgttg attacgccaa cattcgtaac gctaaacgcc ttaaaggcga
     5941 caaactgtgt ctggttaccg acgccaccgc gccagcaggt gccaacattg aacagttcat
     6001 ttttgcgggt aaaacaatat actaccgtaa cggactttgt gtggatgaga acggtacgtt
     6061 aagcggttca tccttaacca tgattgaagg cgtgcgtaat ctggtcgaac attgcggtat
     6121 cgcactggat gaagtgctgc gtatggcgac gctctatccg gcgcgtgcga ttggcgttga
     6181 gaaacgtctc ggcacactcg ccgcaggtaa agtagccaac ctgaccgcat tcacacctga
     6241 ttttaaaatc accaagacca tcgttaacgg taacgaggtc gtaactcaat aagagaaagt
     6301 atgacaccag gcggacaagc tcagataggt aatgttgatc tcgtaaaaca gcttaacagc
     6361 gcggcggttt atcgcctgat tgaccagtac gggccaatct cgcggattca gattgccgag
     6421 caaagccagc ttgcccccgc cagcgtaacc aaaattacgc gtcagcttat cgaacgcggg
     6481 ctgatcaaag aagttgatca gcaggcctcc accgggggcc gccgcgctat ctccatcgtc
     6541 accgaaaccc gcaatttcca cgcaatcggc gtacggcttg gtcgtcacga cgccaccatc
     6601 accctgtttg atctcagcag caaagtgctg gcagaagaac attacccgct gccggaacgt
     6661 acccagcaaa cgctggaaca tgccctgttg aatgccattg ctcagtttat tgatagctac
     6721 cagcgcaaac tgcgcgagct gatcgcgatt tcggtgatcc tgccagggct tgttgacccg
     6781 gacagcggca aaattcatta catgccgcat attcaggtag aaaactgggg gctggtagaa
     6841 gctctggaag aacgttttaa agtgacctgt ttcgttggtc acgatatccg tagtctggcg
     6901 ctggcagagc actacttcgg tgcaagtcag gattgcgaag actccatttt agtgcgtgtc
     6961 catcgcggaa ccggggccgg aatcatctct aacgggcgca tttttattgg ccgcaacggc
     7021 aacgtcggtg aaattggcca tattcaggtc gaaccgctgg gtgaacgctg ccactgcggc
     7081 aactttggct gcctggaaac tatcgccgcc aacgctgcca ttgaacaacg ggtgttgaat
     7141 ctgttaaagc agggctacca gagccgcgtg ccgctggacg actgcaccat caaaactatc
     7201 tgcaaagccg cgaacaaagg cgatagtctg gcgtcggaag taattgagta tgtcggtcgt
     7261 catctgggta aaaccatcgc cattgctatc aacttattta atccgcaaaa aattgttatt
     7321 gccggtgaaa tcaccgaagc cgataaagtg ctgctccctg ctattgaaag ctgcattaat
     7381 acccaggcgc tgaaggcgtt tcgcactaat ctgccggtgg tacgttctga gctggatcac
     7441 cgctcggcaa tcggcgcttt tgcgctggta aaacgcgcca tgctcaacgg tattttgctc
     7501 cagcatttgc tggaaaatta atgtgctttt atagtggcgc ttattgttgt caatattctg
     7561 ggtagtccat gaccattaaa aatgtaattt gcgatatcga cggcgtgctg atgcacgata
     7621 acgtcgccgt accgggtgca gcggaatttt tgcacgggat tatggataaa ggcctgccgc
     7681 tggtgttgct gaccaactat ccttcgcaga ctgggcaaga tctggcgaac cgctttgcca
     7741 ccgcaggtgt cgatgtacct gacagcgtgt tttatacctc tgcgatggcg actgccgatt
     7801 tcctgcgtcg ccaggaaggc aagaaagcgt atgtggtggg cgaaggcgca ctgattcatg
     7861 aactgtacaa agccggtttc actattaccg atgtgaaccc tgatttcgtg attgttggcg
     7921 aaacgcgttc ctacaactgg gacatgatgc ataaagcagc ctatttcgtc gctaacggtg
     7981 cacgctttat cgccaccaac ccggatactc acgggcgcgg tttttatccg gcctgtggcg
     8041 cgttgtgtgc agggattgag aaaatctccg ggcgcaaacc gttctatgtt ggtaagccga
     8101 gtccgtggat tatccgcgcg gcattaaaca aaatgcaggc acattcggaa gaaacggtga
     8161 ttgtcggcga taacttgcgc accgacattc tggcgggctt ccaggccggt ctggagacga
     8221 ttctggtgct ttctggtgtt tcatcgctcg acgatatcga cagtatgccg ttccgcccaa
     8281 gctggattta cccgtcagtc gctgaaatcg acgttatctg aatagcaacc acgtctgcgg
     8341 acgtggttgg ctctgatgac aaacgccaaa ctgcctgatg cgctacgctt atcaggccta
     8401 cgcagctcct gcaatatatt gaatttttat gcttttgtag gccggataag gcgttcacgc
     8461 cgcatccggc atgaacaaat cgcactttgt cagcaatttg gcccccgtaa aatataacca
     8521 ttcaataaaa cccgctaaaa attatcgatt catcaataaa tacatcctca taccagacct
     8581 tttttcacca atcgacaatc gccattacgt tttttatttt tccactgcca aatagcttgc
     8641 gcacagtgct gctttgcggc atttttttaa acaagcaaac acaacaagca acaaatacct
     8701 ggttaacgga gaaggttatg tgttcaattt ttggcgtatt cgatatcaaa acagacgcag
     8761 ttgagctgcg taagaaagca ctcgagctgt cacgcctgat gcgtcatcgt ggcccggact
     8821 ggtccggtat ttatgccagc gataacgcca ttctcgctca cgaacgtctg tcaattgttg
     8881 acgttaacgc gggggcgcaa cctctctaca accaacaaaa aacccacgta ctggcggtaa
     8941 acggtgaaat ctacaaccac caggcattgc gcgctgaata tggcgatcgt tatcagttcc
     9001 agacagggtc tgactgtgaa gtgatcctcg cgctgtatca ggaaaaaggg ccggaatttc
     9061 tcgacgactt gcagggcatg tttgcctttg ccctgtacga cagcgaaaaa gatgcctacc
     9121 tgattggtcg cgaccatctg gggatcatcc cactgtatat gggctatgac gaacacggtc
     9181 agctgtatgt ggcctcagaa atgaaagccc tggtgccagt ttgccgcacg attaaagagt
     9241 tcccggcggg gagctatttg tggagtcagg acggcgaaat ccgttcttat tatcatcgcg
     9301 actggttcga ctttgatgca gtgaaagata acgtgaccga caaaaacgag ctgcgtcagg
     9361 ctctggaaga ttccgttaaa agccatctga tgtctgatgt gccttacggt gtgctgcttt
     9421 ctggtggtct ggattcctca attatttccg ctatcaccaa gaaatacgcc gcccgtcgcg
     9481 tggaagatca ggaacgctct gaagcctggt ggccgcagtt acactccttt gctgtaggtc
     9541 tgccgggttc accggatctg aaagcagccc aggaagtggc aaaccatctg ggcactgtgc
     9601 atcatgaaat tcacttcact gtacaggaag gtctggacgc catccgcgac gtgatttacc
     9661 acatcgaaac ctatgatgtg accactattc gcgcttcaac accgatgtat ttaatgtcgc
     9721 gtaagatcaa agcgatgggc attaaaatgg tgctgtccgg tgaaggttct gatgaagtgt
     9781 tcggcggtta tctttacttc cacaaagcac cgaatgccaa agaactgcat gaagagacgg
     9841 tgcgtaaact gctggccctg catatgtatg actgcgcgcg tgccaacaaa gcgatgtcag
     9901 cctggggcgt ggaagcacgc gttccgttcc tcgacaaaaa attccttgat gtggcgatgc
     9961 gtattaaccc acaggataaa atgtgcggta acggcaaaat ggaaaaacac atcctgcgtg
    10021 aatgttttga agcgtatctg cctgcaagcg tggcctggcg gcagaaagag cagttctccg
    10081 atggcgtcgg ttacagttgg atcgacaccc tgaaagaagt ggctgcgcag caggtttctg
    10141 atcagcaact ggaaactgcc cgcttccgct tcccgtacaa cacgccaacc tctaaagaag
    10201 cgtacttgta tcgggagatc tttgaagaac tattcccgct tccgagcgcc gctgagtgcg
    10261 tgccgggcgg tccttccgtc gcgtgttctt ccgctaaagc gatcgaatgg gatgaagcgt
    10321 tcaagaaaat ggacgatccg tctggtcgcg cggttggtgt tcaccagtcg gcgtataagt
    10381 aagaaatgcg aaacagcccc ggtgaaatcc ggggctgttt cagttattga ggtgcttgtt
    10441 gattctgata tgcagtaagg aaatgttccg ggtttgccac atcactgttt gacaactcat
    10501 cactgtaggc cagtatttca tagattaatt tcccaccact attgttaaat tccgaatttc
    10561 atcctgtatg acaaaagaat ggctgtaggg tgggaaaaaa cacaaagtaa acaataattg
    10621 acgaatatag cgccacgctg ttcgcaacct aaccaaacag tcactttcga gcaattttcc
    10681 ttgaaaaaga ggttgacgct gcaaggctct atacgcataa tgcgccccgc aacgccgata
    10741 aggtatcgcg aaaaaaaaga tggctacgta gctcagttgg ttagagcaca tcactcataa
    10801 tgatggggtc acaggttcga atcccgtcgt agccaccatc ttttttgcgg gagtggcgaa
    10861 attggtagac gcaccagatt taggttctgg cgccgcaagg tgtgcgagtt caagtctcgc
    10921 ctcccgcacc attcaccaga aagcgttgta cggatggggt atcgccaagc ggtaaggcac
    10981 cggtttttga taccggcatt ccctggttcg aatccaggta ccccagccat cttcttcgag
    11041 taagcggttc accgcccggt tattggggta tcgccaagcg gtaaggcacc ggtttttgat
    11101 accggcattc cctggttcga atccaggtac cccagccatc gaagaaacaa tctggctacg
    11161 tagctcagtt ggttagagca catcactcat aatgatgggg tcacaggttc gaatcccgtc
    11221 gtagccacca cattctgaat gtgtcgaata agttcggcaa attcaaaacc
//
LOCUS       DAPGOF010000160        11128 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.161, whole genome shotgun sequence.
ACCESSION   DAPGOF010000160 DAPGOF010000000
VERSION     DAPGOF010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11128
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.161"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            390..1874
                     /locus_tag="RHV13_003752"
     CDS             390..1874
                     /locus_tag="RHV13_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HDV1243233.1"
                     /translation="MSFGQPCDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFFLTLAESGERKTAVDKLLMKPLYQQEMQLYSRYK
                     SELAVWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEVLQKNSAEEPVRYKFI
                     FNDATTAAIKNQLCGKWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VIPGGSLTAFHERLMELARGSIEKSSEDERYCLHFSPEAQKIFIEHYNVLEQDLSPSG
                     PLSPFRGHVSKKTENIARIAALFQYFSYGEGKISADIMTSAVVISSWYTDEYKKLFAL
                     PDESELQQKDAEELFDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNALLNILES
                     QFRLSVVPEGKTMYVLLPQIASLKLSDVSGIFTSGYHYNKLRAK"
     gene            complement(2196..2798)
                     /locus_tag="RHV13_003753"
     CDS             complement(2196..2798)
                     /locus_tag="RHV13_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243234.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNCLDDWTENLKRK
                     SEKELEEWIINRQKQSAALTDLMDQYVLLSYRTTVDDKRT"
     gene            complement(2893..3099)
                     /locus_tag="RHV13_003754"
     CDS             complement(2893..3099)
                     /locus_tag="RHV13_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HDV1243235.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGGFPAPIKM
                     ERSSRWLKSEVEAWLQARIAQSRP"
     gene            3856..3981
                     /locus_tag="RHV13_003755"
                     /pseudo
     CDS             3856..3981
                     /locus_tag="RHV13_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850858.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
     gene            4697..5266
                     /locus_tag="RHV13_003756"
     CDS             4697..5266
                     /locus_tag="RHV13_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HDV1243236.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRFPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPGSLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKVHCTGERNFGCSRG"
     gene            5432..5833
                     /locus_tag="RHV13_003757"
     CDS             5432..5833
                     /locus_tag="RHV13_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243237.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQAAPYAHYTCHLCGSALRYHPQY
                     DTELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGGFSIPRTTQEEICEF"
     gene            5821..6255
                     /locus_tag="RHV13_003758"
     CDS             5821..6255
                     /locus_tag="RHV13_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HDV1243238.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            6601..6990
                     /locus_tag="RHV13_003759"
     CDS             6601..6990
                     /locus_tag="RHV13_003759"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HDV1243239.1"
                     /translation="MSDMQKNVTPGRRKGCPNYPPEFKQLLVAASCEPGISISKLALE
                     NGINANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEV
                     TFRHGTLRFNGNVSEKLLTLLIQELKR"
     gene            6987..7334
                     /gene="tnpB"
                     /locus_tag="RHV13_003760"
     CDS             6987..7334
                     /gene="tnpB"
                     /locus_tag="RHV13_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDV1243240.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKNDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            7384..8769
                     /locus_tag="RHV13_003761"
     CDS             7384..8769
                     /locus_tag="RHV13_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HDV1243241.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYCQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKGIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLFFGSGHGGERGALLYSLIGTCKLNDVDPESYLRHVPGVIADWPVNRVSE
                     LLPWRIALPAE"
     gene            complement(9008..10366)
                     /locus_tag="RHV13_003762"
     CDS             complement(9008..10366)
                     /locus_tag="RHV13_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HDV1243242.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASTFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            10648..10779
                     /locus_tag="RHV13_003763"
                     /pseudo
     CDS             10648..10779
                     /locus_tag="RHV13_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         2733 a         2763 c         2958 g         2674 t
ORIGIN      
        1 cctgtccata aaatattgta ctcagcattc agtgcaggaa ctgtagtctt ctttatttta
       61 ttcttcttca ggaggggggg tgattcctga aggcgttatt cccggtgttc tgtttatgat
      121 tcttcccggc aggaaaccgg tatctgcaat acccgggatg atgctttttg ttcgtacgtg
      181 gttgtgtaaa atttgaatag tggatgtgct ccgaagggaa ggaggggaca cacagattca
      241 ttttgtcttt cttcagcagc aaaatacacc gtggttatta tttaaccatt aaatcaacaa
      301 aaattttacc tcatattgat ttgactgcta tttttgctat taatagccag aattgcagtg
      361 cttttatatc tgtgtatagt gaggttacta tgtcattcgg tcagccttgt gatgagtttc
      421 ccctgagttc gctgcctcca ctcattcgtg atgccgttat tgaagctcag caaattacgc
      481 aggctcccct tgggcttgtg gcagcgtctg ccctgggcgc tgtttcactt gtctgccaga
      541 atctgattga tgtctgcaga ctgaatacac tgcgggggcc ggtctctctt tttttcctga
      601 cgcttgccga gtccggagaa cgaaaaaccg cagtggacaa attattaatg aagcccctgt
      661 accagcagga gatgcagtta tattccagat acaaaagtga actggctgtc tggaaaaaca
      721 aggaagagct tttaaaggct cagaaaaaag cattgttgtc aaaactgaat aaagaactcc
      781 gcaaaggcgc ggatgagtca gaaaccctca gacaactgga agtacttcag aaaaacagtg
      841 cggaagaacc tgtgaggtat aaattcattt ttaatgatgc cacgactgcg gcaattaaga
      901 atcagctttg tggtaaatgg cgttcagttg gtattatgtc agatgaagcc ggtattatat
      961 ttgatggata taccctgtct gagttgcctt ttattaacaa aatgtgggat ggctctgtat
     1021 tgtctgtgga cagaaagaat gaacccgaac agatgattga aaatgccaga atgacgttat
     1081 ccctcatggt gcaacccggt ctttttgacc gatatatgga gcgaaaaggt tctgttgcca
     1141 gggacagtgg ttttcttgcc cgttgtctta tcagtaaacc tgcaaccacg caggggaaac
     1201 gctttattaa cggagctgtg attccgggag gatcattaac ggcatttcat gaacgattaa
     1261 tggaactggc cagaggcagt attgaaaaat ccagtgagga tgaacggtat tgtctgcatt
     1321 tttcgcctga agcgcaaaaa atatttattg agcactataa tgttctggag caggatttat
     1381 ccccttccgg acccttaagc ccgtttcggg gacatgtttc aaaaaaaaca gaaaacattg
     1441 caagaattgc agcgttattt cagtatttca gctatggtga agggaaaatc tctgccgata
     1501 tcatgacgtc tgctgtcgta ataagttcgt ggtacacaga tgaatataaa aaattatttg
     1561 cgttacctga tgaatctgaa ctgcagcaga aggacgccga ggagctgttc gactggctca
     1621 ttgaggagtg ccgcggagaa tgcccgcccc gtgtcaggaa aaattatatt ctccagtgtg
     1681 gcccgggccg gttcagaaac aggaaaaaac tgaacgcatt gctgaacatt cttgaatctc
     1741 agttcaggct ttctgtcgtg ccggaaggaa aaaccatgta tgttctgctt ccccagatag
     1801 ccagtttaaa gttgtctgat gtctctggta tttttacctc gggttatcac tacaataagc
     1861 tgagagcgaa gtaaccggtg agcagttttt ctccattatg cttcagactt agatgattga
     1921 ttattatggg gatttcatca ccgatattca gatgtatgaa tatcggtgat gaactaaagc
     1981 tcaggagctg cgtggtggtg ataaatcaac aggcatataa ctgatacccg ccggtcagaa
     2041 gttccagcct gcgactttcc tgtctgcgtg cccgcagtcc ggcgttcagg tgcagcgtgt
     2101 gacagaacgc acggagagcg aaacggcagg cagacaggcc gttcagtcag ccctggcacc
     2161 ctgcccggat accagcttcc ctgaaacaac tggcatcagg tgcgcttatc gtcaaccgtt
     2221 gtgcggtatg acaggagcac gtactgatcc atcagatccg tcagtgctgc gctctgcttc
     2281 tgacggttga tgatccattc ctccagctct ttttctgatt tccgtttcag attctccgtc
     2341 cagtcgtcaa ggcagtttcc ggtcattaaa tcaagccagc aaagccagac tagtttgagg
     2401 cgcaactccg tgccaccaaa ctgacggaag aggctgatca gtttatttgc cagagaacgc
     2461 tgcctcagac gttcacattc agacaggaga atgccttcca gcatcggatg atgaaccagc
     2521 agatgttcac tggtgcataa attcactatc agatcatcat ccaggggcgg tgtggcccgt
     2581 ttgtcagggc cataaatcag ccggtgcagg ctctggggta cgatggcaat ctccggtttt
     2641 tcaccattga atgacaccca accgggcgtc agattatgga agtagtagat attcggatga
     2701 cacacgcccc aggatacggc gtgccgggtg aagccggtca gccatttctg gtagctttcg
     2761 tggagccatt cgtagcgggt ctgatactgt tgtttcattt tcatattcct cacggggata
     2821 taacaggcag cagaatgccg ccggcaggcc ggagattacg tctgctgcgg gtgaacggat
     2881 aacggtcaga aattacggac gggactgtgc aatacgcgcc tgcagccagg cttccacttc
     2941 acttttcagc cagcgggagc tgcgctccat tttgatgggg gcaggaaagc ccccatcttt
     3001 gatgagtttg taaaaccact tatcggttaa gccggtaagt tgagtgataa acgccatgtc
     3061 gaccatctgg tcatccatca gcgaaacagg ggtagccatg agaatgttcc tccggatgtt
     3121 gttaaaccag ccggtgtggt catgaaaaca gcgcgccacc ttcactggca taacaaccgg
     3181 ggagaaacgt aaaaaataat cgggaatgtg gctgtccgga gataaacggg gggattgcga
     3241 tggcagagcc gtagcggaat cccgggtggt tgttgcgaaa agatgcttcc gcacgcacgc
     3301 gcgataaagg agggaggact ttcgtgagac aaggggaaaa ctagcgttct gataattgca
     3361 gagttgtttt cggaggtggt tacagtgagt cagcccgttc ctggcgagga ggagactgat
     3421 atcaccgatg ggataaggct gtctgattgg tattataaat ataagaagtc atcagagagt
     3481 aacgtaaagg atttgccaga gtaagctaag agagtaaatc tctccggtaa cagagcgctg
     3541 tgaatcgttc atgtatcaga gtgcagatac atgaacgctg atgcgtaaaa tcagcagtga
     3601 cacgccagct ccccctgtgc ccattcctgt ataagacatg aactgagcgt attaatgctc
     3661 ctgccctgag actgagccgg tgagtgaggg gctgagaaat ccctcttgac actgctctaa
     3721 accagaaata cttccccggt tgttgcggtg cgtccggatc cccgataacg gaacactaag
     3781 gctgtattgc gggacacctg gtaagtggca gcccgtaata cgtcaggaga cgcgggaaaa
     3841 gtgaattccc acacgcgtta taaacttacc cttgcggagc tggaagcctt taactctgcc
     3901 gttgacaacc ggctggcaga actgacaatg aacaaacttt acgatcgcgc gccggcttcc
     3961 gtctggaaat atgtcacctg aatattgaag cagcgctacg atgactccgg tgttatctgc
     4021 accggaggac cgggaacatt aagcggtata gcgacaacaa agaattcgaa atcaaaaggg
     4081 aatggtgccg gaggcgtggg cggtaatagg ctgattttca ggtattaccg gcctgtatct
     4141 gctgaagggg gctcgtattt gtcggggctg cgtattccgc tcgattcgat cacggattgg
     4201 cggaatacct aactgtgatt gattcaggta ccattttgca gggatccggt gcgttactgt
     4261 ctgacacaac aaacgcagcg ggtcgctctg ctccgccgat tcctgataat ccctccccag
     4321 taacctactg caacagtaca aatacactga cgcctgaaac ggtaaccaga atcagcacca
     4381 gcagtaactc agtaaacaga gcagacgatc ggtcttccgg taataaccgg aaggaacgac
     4441 agcgccacaa tacaggcaac acactgtatt atgagtaatc gaattaccgc aatccccgca
     4501 tttctttttg cgtatcacga tagctccctc gccacgcctt atccgtaacc ggttttttac
     4561 attaaaaaaa acaacgccgg gaattcaatc gtcagtccgg agacgaccgt tcgggttatc
     4621 acagagagcc tgagcagggg ggctgcgcat tttttacgat gtggctattc cgtatgaacc
     4681 atacggagat ataaccatga cctacaaata caaccccttc tggcagcaac gtattcgtga
     4741 gacggtgcgg cacgcactga atgttcatcc ccgcctgacg gcattgcggg ttgacctgcg
     4801 tttcccggat gtaccggcag caacggacgc agctgtgata tcccgcttca tcaatgccct
     4861 gaaagcccga atcgacgctt accagaaacg taagcatcgg gaaggtaaac gcgtgcatcc
     4921 cacaaccctg cattacgtct gggcccggga gtttggggag tgcaaaggta aaaaacacta
     4981 tcacctgatg ctgctggtca accgggatac ctggtgtcgt gccggtgatt accgcgctcc
     5041 gggatcactg gccgggatga ttaaacaggc gtggtgcagt gccctgggag tggatgtcgg
     5101 gtgccatgcc acgctggtgc attttccggc ctggccggcg gtgtggctgg cgcgtaatga
     5161 tgacaccggc tttcagcaag tgctggaacg tgctgactat ctggcgaagg agcataccaa
     5221 agttcactgc accggtgagc gcaactttgg ctgcagtcgt ggctgagcca gacagacgag
     5281 ttatctgact tcagtgtgat gacgtgttca cggccaccac actgattttc ccttatgaga
     5341 ctgaactgaa accgccattc acaccggacc acgctgcccg taccgggacg gctgttgcct
     5401 gcgtgtcgtc tgacggtaag gaacatatac tatgtacgca aaatcctttc tcgctcttga
     5461 tggcaacgga cgtctgacgg gcgcccgtac agcacaggcc gcaccttatg ctcactacac
     5521 ctgccacttg tgtggcagtg cactcagata ccatccgcaa tacgacactg aacttccctg
     5581 gtttgaacac actgacgaca ggctgacaga gcacggtcaa cagtgccctt atgtcaggcc
     5641 ggagcgcaga gaaatacagt tgattaaacg tctgcagcaa ttcgtaccgg atgccttacc
     5701 cgtggtgcgt aaagccagct ggcactgcag acaatgtcac cacgattatt atggggagca
     5761 gtactgcaca cactgccaga ccggaggatt cagtattccc cggacaactc aggaggaaat
     5821 atgcgaattc tgaactgtta tatggcgaat gacagcaaag gccattttgt tacggcgaaa
     5881 gaagctgcga agcacaaccg acaggacgtt ttgtgctgtg tgtcctgtgg atgcccgtta
     5941 acacttcagc ggggcaatga cggacaacca ccgtggtttg aacatgacca gatgactgtc
     6001 gctgaaaaaa tcctgctgcg atgtacctgg cttgacccgg cggagaaaga ggcccgtcgt
     6061 ttgcatctgc agggcatgac ggttccggat tatacggtga aggtgagaaa gtggttttgt
     6121 gtgatgtgtg acgaagatta tgagggggaa aagtgctgcc cacgctgcgg taccggggta
     6181 tacagcaggg cgtgggggcg gcaggaggtg ccgtcggaag atgccagggc tgataatccg
     6241 ttacagaggc tgtaatggtt gcctccggag cagtttgcgg tgatgccttt cccgggaatg
     6301 gattgccagt gcggggactg tggagcttaa ttccggtgtt ggtggccttt ccgttgattt
     6361 tatgccaaca gccccctgtg actgacaggc tgcctcgtca ttccattcgt atctccagta
     6421 acagggggtt gtattgtatt ttattgtgcc ggttcaggtg tgcgtttccc ggcgcgtctg
     6481 caccggctta accaaattca acagggataa aatagtggta agcgtacagc ctgaaccgtc
     6541 tggtcagaat ctgacgaatt agacaaagtg gtgtccacca aataagtagt gggaaccaaa
     6601 gtgtcagata tgcagaaaaa tgtgactccc ggcaggcgaa aaggctgccc taattatcct
     6661 cccgaattta aacagctgct cgttgctgcc tcctgtgaac ccgggatatc catctcaaaa
     6721 cttgctcttg aaaatggcat taacgccaat ctgttgttca aatggcgaca acaatggcgc
     6781 gagggaaagc tgctattacc ttcttcagag agcccccagc tacttcctgt gactctcgat
     6841 gcagctgccg aacagccaga atcgctcgca gaggacccgg aaaccctcag tatcagctgt
     6901 gaggtaacgt tccggcacgg gacgctccgc ttcaatggca atgtcagcga aaagctcctg
     6961 actctgctga tacaggaact gaagcgatga tcccgttacc ttccgggacc aaaatttggc
     7021 tggttgccgg tatcaccgat atgagaaatg gcttcaacgg cctggctgcg aaagtacaaa
     7081 cggcgctgaa aaacgatccc atgtccggcc atgttttcat tttccggggc cgcagcggca
     7141 gtcaggttaa actgctgtgg tccaccggtg acggactgtg cctcctgacc aaacggctgg
     7201 agcgtgggcg cttcgcctgg ccgtcagccc gtgatggcaa agtgttcctt acgcaggcgc
     7261 agctggcgat gctgctggaa ggtatcgact ggcgacagcc caagcggctg ctgacctccc
     7321 tgaccatgct gtaaatctct ttatcctggt tgtcacagaa taagcccggt aaaatacggg
     7381 cttatgaacg acatctcttc tgacgacatc ttcctgctaa aacagcgcct ggccgaacag
     7441 gaagcgctga tccacgccct gcaggaaaag ctgagcaacc gggagcgcga aatagaccat
     7501 ctgcaggcgc agctggataa actccgccgg atgaacttcg gcagtcgttc cgaaaaagtc
     7561 tcccgccgta tcgcacaaat ggaagccgat ctgaaccggc ttcagaaaga gagcgatacg
     7621 ctgactggta gggtgtatga cccggcagta cagcgtccgt tgcgtcagac ccgcacccgt
     7681 aagccgttcc ctgaatcact accccgtgac gaaaagcgac tgttgcctgc ggcgccgtgc
     7741 tgcccgaact gcggcggttc actgagctat ctgggcgagg ataccgccga acagctggag
     7801 ttgatgcgta gcgcattccg ggttatccgg acggtacggg aaaaacatgc ctgtactcag
     7861 tgcgatgcca tcgtgcaggc acctgcacct tcgcggccca tcgagcgggg tatcgccgga
     7921 ccggggctgc tggcccgcgt gctgacctcg aagtatgcag agcacacccc gctgtattgc
     7981 cagtcagaaa tatacggccg gcaaggtgtg gagctgagcc gttcactgct gtcgggctgg
     8041 gtggatgcat gctgccggct gctgtctccg ctggaagagg cgcttcatgg ctatgtcatg
     8101 actgacggca aactccatgc cgatgatacc ccggtccagg tactgctgcc gggtaataag
     8161 aagacgaaga ccgggcggtt gtgggcgtat gttcgtgatg accgcaatgc cgggtcagcg
     8221 ttggcacctg cagtgtggtt cgcttacagc ccggacagaa aaggcatcca tccgcagact
     8281 catcttgctt gcttcagcgg tgtgctgcaa gcggatgcgt acgccgggtt caacgagctg
     8341 tatcgcaatg gtgggataac ggaagctgcc tgctgggctc atgcccgccg aaagatccac
     8401 gatgtgcacg tccgcatccc gtcagcactg acggaagaag ccctggagca gatcggtcag
     8461 ttgtacgcca tagaggcgga tataagggga atgccggcag agcagcggct tgctgaacgt
     8521 cagcgaaaaa cgaaaccgct gttgaaatcc ctggaaagct ggttgcgtga aaagatgaag
     8581 accctgttct tcggctctgg ccatggtggt gagcggggag cgctactgta cagcctgatc
     8641 gggacgtgca aactgaatga cgtggatcca gaaagctacc ttcgccatgt gcctggcgtc
     8701 atagcagact ggccggtcaa ccgggtcagc gaactgcttc cgtggcgcat agcactgcca
     8761 gctgaataac acatccccgt caatacggcc ctcgctgtac gcttactttt gttcggactg
     8821 cttatggcct ttttccactg gtgtagcagg aaagttaacc ggctgccacc atgagatgac
     8881 gtgtaaaccg tgcaggcatg ttttcacgtg gtgacgcttt cattgggggt ctacgccgga
     8941 accccaatat tgttcacata catgaatttg aaaaactaag cgatactgct gtccgtacct
     9001 tataaactta tttcaacgct tccggcagcg tcacaatcca cagttcactg tcagactccg
     9061 gtttctccag tggcttcact ctgagggtcg tttcaacctg cttgtcgtca agcgtcagct
     9121 ttccttccgc gttcacccga taatccttga atgtcttgcc ctcgaccgga tgttgcatcg
     9181 ccaccttcac gccaaagtcg ttatcgttcg ccaccgccag cgttttgctg tcgatcagcg
     9241 ccaacccttc agccttctcc tgctgccagc ccaaggagcg cagatcaaca acctgcgttt
     9301 tctgcgcaag ggtaatgccg cgctgtgaca gggttttctc atcatcaaac tccggatact
     9361 cacccggctt gtcgaagcct gacaggtcgc tcgccttatt gagatcgacc tcgtaaatca
     9421 ggttacgcat tctgttgttt ttatctcttc cctgttcgat cagcaggatg tgctggttat
     9481 cgagcgccac gatgtctccg attttagcgt cgctgttttt gctgtaggcc gcgctgtcga
     9541 taggataacc gtacatcgcg gtttttccgc ttgccggatc gaagctcacc agacgcgtaa
     9601 acagcgcctt tttcttgctc ttagcgtcga tatccagcgt actttgcacg gcgacaataa
     9661 tacgcccgtc cggcatacgg gtgaggcctt caaagccgcg gttcgcctga cgccatttga
     9721 ggatatttgg caaaccgccc gcaatcgctt tttccccttc cgccgcctgc ggaccgtgga
     9781 ttgccaggat tttcccttta ctgtcgatgt taatcaggaa cgggccatac tcatcgcaca
     9841 gccagtaacc tcctttacca tccggagtga tcccttccgt atccagcccg cgattatccc
     9901 ccttcagtct atgtaacgtg tcgctgaagg ccacttcatt ggtggagcca atcacgtcgc
     9961 ttgccagcgg cagaccgttg atcgcgcctt tatcgtcatg cagaggacgg ggatcgatgg
    10021 cctccgcttt gccgttttgt acacgaatcg tcatcagcag cggcgcgaaa tctggcgtaa
    10081 cgaaaatttt agtttcgttt ttcccctctt tcggtgaatc cgcgttcgga ccgcgatcgg
    10141 taatggttgc gaatgtcagg gcatcgccct gtttaccggt gaacagcagg ccagaaccta
    10201 tcccaaccgg aaggccgttc gggaatgtac tggcaaatgc gccggcgtag ttcacgtgcg
    10261 tgccttctgg aaaactgacg acatagcgct cagccgtagg ttgtgccgcc agggcagaga
    10321 aagagagcgt acaaccgatt agcacgggaa taatttttct tttcatattg ttagcatcca
    10381 taaagtgaag atcacactac accataaaga agaaacgagt caggaaaatg acatcgaatg
    10441 gcaacatgcg cgggcctgag gccaaagaaa tgtaaaacac tgttgttatt tgtaacaaag
    10501 gaaacattta cggaaattat ttgtctttct tcgtgatcaa aaatgctgtg tcttgttgga
    10561 ttggagtgat gagggctgcg tttaggaaac gccctatatt gtacggttgc attatcgcgg
    10621 taggggtaat gcacgtgtca gtatgatgag ctgaccacac tcacgtgggt ggactggtat
    10681 aacaatcgac gattgctgga aaggcagggc catatcccac cggcagaagc agaaaaagct
    10741 tattatgctt ccatcggaaa cgatgatctg gcagcctgag ttcacagata aaacactctc
    10801 caagaaaccc ggggcggttc aattgctgga taagatcata acgatacacc gggttaaaca
    10861 ggtggtcact gcttcactga agctgcgtga ttatgatggt caggtagcag aagctatgcc
    10921 catgatacaa aaatgacgaa agccgaaatg tctgaaagcg tgtgtattac ctgaaaatat
    10981 gaccggctcc ggggtacgta accgattcaa caaagccaat cgatattaca aaacgagagg
    11041 gactatatat ttcgttttca gataagactc cttgagagat tatcttttat gtaaaatctc
    11101 aaatatcacc tgatgtaatg tcaatgat
//
LOCUS       DAPGOF010000161        11117 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.162, whole genome shotgun sequence.
ACCESSION   DAPGOF010000161 DAPGOF010000000
VERSION     DAPGOF010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11117
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.162"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            114..335
                     /locus_tag="RHV13_003764"
     CDS             114..335
                     /locus_tag="RHV13_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243243.1"
                     /translation="MFKDKTVAAEISALMLEIGAKLDNSVSLVQQTCDESELDTYRSA
                     IGEIMGRMLIDIMNPIYKQHPELKPKELN"
     gene            complement(339..1295)
                     /locus_tag="RHV13_003765"
     CDS             complement(339..1295)
                     /locus_tag="RHV13_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HDV1243244.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(1295..2536)
                     /gene="clsB"
                     /locus_tag="RHV13_003766"
     CDS             complement(1295..2536)
                     /gene="clsB"
                     /locus_tag="RHV13_003766"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HDV1243245.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAVDCKQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVDTENTGVKP"
     gene            complement(2533..3294)
                     /locus_tag="RHV13_003767"
     CDS             complement(2533..3294)
                     /locus_tag="RHV13_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDV1243246.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            3427..3837
                     /locus_tag="RHV13_003768"
     CDS             3427..3837
                     /locus_tag="RHV13_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HDV1243247.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(3799..4905)
                     /locus_tag="RHV13_003769"
     CDS             complement(3799..4905)
                     /locus_tag="RHV13_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV1243248.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(4916..6049)
                     /locus_tag="RHV13_003770"
     CDS             complement(4916..6049)
                     /locus_tag="RHV13_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV1243249.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(6042..7778)
                     /locus_tag="RHV13_003771"
     CDS             complement(6042..7778)
                     /locus_tag="RHV13_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV1243250.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPITRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(7771..8766)
                     /gene="hlyD"
                     /locus_tag="RHV13_003772"
     CDS             complement(7771..8766)
                     /gene="hlyD"
                     /locus_tag="RHV13_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HDV1243251.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            complement(8769..9440)
                     /gene="cecR"
                     /locus_tag="RHV13_003773"
     CDS             complement(8769..9440)
                     /gene="cecR"
                     /locus_tag="RHV13_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HDV1243252.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            9669..11033
                     /gene="rhlE"
                     /locus_tag="RHV13_003774"
     CDS             9669..11033
                     /gene="rhlE"
                     /locus_tag="RHV13_003774"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HDV1243253.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQLRRGEGGAKSASAKPAEKPSRRLGDAKPVGEQQRRRRPRKP
                     AAAQ"
BASE COUNT         2610 a         3045 c         2942 g         2520 t
ORIGIN      
        1 actaattctc caaacaaccg atttgatcta tacaaaaaga gatgattatc ccttaagtga
       61 tgaaatggaa aacgtggttt cctttgaatt tagcaataat aaggatttgt agaatgttta
      121 aagataaaac tgtcgcagca gaaattagtg cattaatgct tgagattggt gcgaaattag
      181 ataattccgt ttcacttgtt cagcaaactt gcgatgaatc tgagcttgat acttatcgtt
      241 ccgccatcgg tgaaataatg gggcgcatgc taatcgacat catgaacccc atctataaac
      301 agcatcccga acttaaacca aaagaactta actgaacttc acatcgccgc ttcatttttc
      361 gcccgcagct tcttcgcctg gctttccagc aacaggtaac agatcagcgc cagcagcagc
      421 gggataaagt aatacagcac acggtaagcg agtagggcgg cgataattgt gcccttggat
      481 gtatgctccc cagccagtag tgcgataaac accgcttcca gcacaccaat ccccgccgga
      541 atatgcacga tgacaccagc aatactacta accagcaaca cgcccagtac aaagaaatag
      601 ttcacgcttt gaccaagtaa cagccagatt atcgctccca ttaccatcca gttaacgctg
      661 gaaatcagca tctgggcgag ggcgaatttc cacgaaggca gcaccagctt ttgccctttg
      721 atggtcatat ggcggtgctt cgcgaaagcg caaaaccaca aataaaccgc aataatcatc
      781 agtaagccaa tgccgagaat gcgcagcgtg gtttgatcga cataccagtg gtccggcaac
      841 tccaccacgc ctgcggtaaa gataatccct gccagtaaaa tatagcccag ccagttggtg
      901 gtaatactga gcgagaaaat ccgcgtaata gtgctgcccg gtaaccctag tcgggagtac
      961 aaacgatagc gcataccaat gccgccgacc caggtactga gcgtcaggtt gaaggcgtag
     1021 cagataaacg acaccagcat cacctggcgc ttcgccagtt tgtgaccgca gtaaaagcgg
     1081 gcgagcaggt catagcagcc gtaaatcaga tagctgacga ccaccagccc gaccgcacta
     1141 agcagcgcaa cgcgattgta gtcgcggatg accttccaga cctcttccca gtccactttt
     1201 ttggcgtaga ccaccagcaa cacaatcacc gcgataaaaa acagccaggt gaggatcttc
     1261 tttgctaagc gccaacgcgg gtgtgattta ctcatcaggg ttttaccccc gtgttttcag
     1321 tatctacccg atcctgcgtt tccattgtcg gttgtgcggg gggatcaacc tgcgccagac
     1381 gtggtgtgtg tgccggaagc cagccaacca gcgccgggaa gtggcgtaaa aagtggaacg
     1441 ccagcacgct tttggtcagg ttccaccagg tgcgtttcgg cagcatggtc tcatcgacct
     1501 gcttacaatc tacggcaata atgccgttca gattatcgcg cagcgtctgg ttaaaatgac
     1561 gatcgtggat gatgacattt gcttcgagat tcagtgacaa actgagcgga tcgagattac
     1621 tggaccctac tgtcgcccag tgatcgtcca tcaatgccac tttgccgtgg agcgggcggc
     1681 ggcggtactc aaaaacctga acgccacctt taaccagata gttatacagc aagcgcgcgc
     1741 cgactctgac aatcggcata tccggttcgc cctgaatgat cagtttgatc cgcaccccgc
     1801 gccgtgccgc tttacgcaag gcgtgtaaaa atcgatagcc ggggaagaag taggcgttgg
     1861 cgataatgac ttctcgccgt gcctgagtga gcattttcag atagtgacgt tcaatatcat
     1921 cgcgatgttc ttcgttatcg cgccagacca gcaatacctg cgcttctccc ggctggcggt
     1981 tctcttccgc tttgtgatga cgtcgccacc agcgtcgtgc cgcgctctgt ccaggcaggt
     2041 tttccagctc aaactggaga atatcttcga caatcggccc ttcaaggcgt accgcgtaat
     2101 cctgtttagc ctctggaccg tagctggaca tatgctcggc ggagtaattc agcccgccaa
     2161 taaaggctat acgcgcgtcg atcaccacaa ttttgcgatg catccggcga aacacattgg
     2221 tgcgcatacc aaaaaggcga gggcggggat cgtagtagcg gaacactacg ccagctgccg
     2281 tcagttcatt gacaaactca tcgctgagat ccggcgaacc gtagccatcc agcaagactt
     2341 ccgctttcac cccgcgttgc gctgctgcca gtagtgcagc atgcagttgt ttgccgacgt
     2401 catcctcaaa ccagataaac gtttcaagaa tgatgcgttc ttgtgcctcg ccaatcgcct
     2461 taaacaccgc gggataatat tgctcgccgt tttccagcaa ctggatctta ttgccttcgc
     2521 gccagctaca tttcataaat gaatctccgc acttaaaggg gcatgatcag aaaggtgtcg
     2581 ccatgtccgc agcggcaacg cggttggcgc gctggcgctg gcatttttga catagatcct
     2641 gtccagtcgt agtagaggaa attgcaccgg aaacgtgcgc gccgggcgtc cgtgggcgcg
     2701 ggtaaatatc tcatccagcc cggcctgcac ttttaacgga tgattcgctt tttgccgcca
     2761 gtcgttgaaa tcacccgcca ccaataccgg ttcgccgtcc ggtagctcat tcacccattc
     2821 ggcgagcatc gcaagctgcg cttgacggtg cgcctcacgc aggcccagat gtacgcacat
     2881 cacatgaatc gcttttccgg tcatcggcgg cacaatgcgg cagtagagca cgccgcgctt
     2941 ttccgcacca tcgaccgaaa catcgcgatt ctcataatgt tcaatgggat aacgcgacag
     3001 tacggcgttg ccgtgatgcc cttccgggta tacggcattg cgaccgtagg caaaatcgct
     3061 ccacatagtg tcggcgagaa actcgtagtg cgaggtatcg ggccagtttt ccacatgcag
     3121 cggatgaact tcgtgcgcgc ccatcacttc ctgcaggcaa acaatatcgg cgctgacggt
     3181 acgcaccgcg tcgcgaagtt ccggcaaaat gaagcgtcgg ttaaacgcgg taaaaccttt
     3241 gtgaatgttg atggtgagca ccttgaacga aaattgttgt gtttgatcgg gcataatttt
     3301 cctgtctttg cctcttatct cattgaaata gtgtagtcgg cgtcacaaaa aggtgcggtc
     3361 ttacggaatt ttccgtaaag ttcggtactc tgagtaagta gagataaatt cttcaggaga
     3421 gaagccatga agtggcaaca acgtgttcgt gtcgcaactg gtctaagttg ctggcagatt
     3481 atgttgcatt tactggtagt ggcgctgctg gtggtgggct ggatgagtaa gactctggtt
     3541 cacgtcggcg tgggattatg cgcactgtat tgtgtcacgg tagtgatgat gttggtgttt
     3601 cagcgccacc ccgagcaacg ctggcgtgag gtggcagacg tgctggaaga gctgaccacg
     3661 acctggtatt ttggcgcagc gctgattgtg ctgtggctgt tgtcccgtgt actggaaaac
     3721 aactttttgc tggcaattgc agggctggca atccttgccg gcccggcggt agtgtctttg
     3781 ctggcgaaag ataagaagtt acatcacctt acgtctaaac atcgcgtacg ccgctgaccc
     3841 tgtcgtggcc gttatcacca gtagcggcca caaactattc cacacaatat ccagactcgc
     3901 atccttcaaa taaatctgct tggtaatgtc cgtaaagtgg cgaatagggt taatccacgt
     3961 caggttttgc agccatactg gcatgttttc caccggagaa acgtaaccgg aaaggagaat
     4021 ggcgggcatc ataaaaacaa acacgccgat aaacgcctgc tgttgtgtcg agcagagtga
     4081 tgaaatcaac agaccgaatc ccaccagcga taaaccgtaa atcaccatcg taaagtagaa
     4141 cagcgccagc gatccggcga aggggatttg atacgcccag ataccaatcg ccagcacaat
     4201 ggtggcctgg aaggtggcga caattaacgc cggtacggct ttgccgatga agatctgcca
     4261 ggtggtgagc ggcgaaacca gtagctgatc gagcgtacct tgttcacgtt cgcgggcgac
     4321 ggaaagtgaa gtgacgatca ttacgccgat agtggtgatc atggcgatca gcgacggcac
     4381 cacaaaccat ttgtagtcaa gattcgggtt ataccagttg cgtaccacca gctcgctgtt
     4441 gttaggtttc ggttttcctt ccagcagctc ctgctgataa tttttgacga tctgttgcag
     4501 gtagttggcg gcaatttgcg cactgttgga gttacgcccg tcgaggatca actgcaacgg
     4561 cgcggtctgg aaggtatcca gtttgcgcga gaagtcagcc gggaaacgca ccagtagtaa
     4621 cgccttttgt gtgtcgatgg ttgggcggat ctcctgtggg cttttcagca gcagcacatg
     4681 agtaaaggcg ctggcgcggg caaaacgttg ggtcagctcc accgaatgct cgccgttatc
     4741 ttcatcgtag atggcgatgg tggcgttagt cacttccagc gtggcggcga acgggaacag
     4801 aatcacctga ataagcacag gtaaaatcag aatcgcgcgg gtttgcggtt cgcgcagcag
     4861 cgactgcaac tctttgcgga ttaacgtcca taagcgatga aacatgctct tctccctaat
     4921 ccagccgacg tttggttttc agccacgtca ggccgataaa catcaccgcc gaagcgatca
     4981 aaaacagcac gtttaccacc agcaccactg gaatattccc ggcgaggaac aggctttgca
     5041 gggtgctgac gaaataacga gcgggaataa tgtacgtcac cgcgcggatc accgcgggca
     5101 tactgtcgat ctgaaaaata aagccggaaa gcataatcga cggcagaaaa gcggcgttca
     5161 gggcgacctg agcggcattg aactggttgc gggtaatcgt ggaaatcagc agccccatcc
     5221 ccagggtact gagtaaaaac aggctggaga taaaaaacag aatcagcagc gacccgcgat
     5281 acggcacgcc gagaataaac actgacacca gcatacacag caacatcgcc agcatcccga
     5341 gaaagtaata agggatcagc ttacacagca gcagttccgt gcgggtaatc tccgtagaga
     5401 gcagagcctc catggtgccg cgttcccatt ctcgcgccac caccagcgag gtgagaatcg
     5461 caccgatgac cgtcatgatg atggtcaccg caccggggat aatgaagtgc tggctaatcg
     5521 ccgccgggtt aaaccagtag cgggtttgta catcaataag cggttcaaaa gtctgcccgt
     5581 tgtcctccgc tcgctgcatt tgccagatct gccagatccc ttcgacatac ccctgtacaa
     5641 agttagcggt attcggctca ctgccgtcgg tgatcacctg aatcggtgcg gtggcgttgg
     5701 cgcgctccat ctgttccgca aaatccaccg gaataaccac cagaccgcga attttccccg
     5761 cctgcatttt ggcgatcagt tcctgacggt tatcgctgat ggtggcgtcg atgtagggcg
     5821 aaccggtcat ggtgtgggtg aaatccagcg ccgcttcgct acgctgttcc agtaaaatcc
     5881 cgacccgcag cttgctggag tcgaggttaa tgccgtaacc aaaaataaac agcagtagca
     5941 gcgggatcac taccgcaatc agccagctac tcggatcgcg aacgatctgc cgcgtctctt
     6001 taacgcacag cgcccgtacg cgacgccagg acaggatcgg gttactcatt gctatgctcc
     6061 ttatcccagt cgtggatcaa ctgaataaag gcttgctcca tggtgggatc gggttgctcg
     6121 tcgttggccg actgcgcttt caaatcatcc ggcgtgccgc tggcaattaa tttcccacgg
     6181 tacaccaggc cgatgcggtc gcaatattct gcttcatcca taaagtgggt ggtgaccatc
     6241 accgtgacgc ctttctctac catgctgttg atatgtagcc agaattcgcg gcgagtaatc
     6301 ggatcaacgc cggacgtcgg ttcgtcgaga aacagaatgt ccggttcatg catcagcgag
     6361 caggccagtg ccagccgctg tttaaaacct aatggcagtt catcggtggc gtgggaggcg
     6421 atacttttca ggccgaacgc ctcgctcata cgggagattt tttcgttctg cgcccgaccg
     6481 cgtaagccat acacaccaga gaaaaagcgt aaattctgtt cgaccgtcag gttaccgtag
     6541 agcgaaaact tttgcgccat atagccgaga tgctggcgcg ctttaccaga actctctttc
     6601 agatccatcc ccagcaccag cgcctggccg gaagtcggca ccagcaaacc gcacatcatc
     6661 ttaaaggtgg tcgatttacc cgcgccgttt ggccccagca aaccaaaaat ctccccacgt
     6721 ttaacggcaa agttgacgtg atcggtggcg gcaaaatcac caaatttctt ggtcagttct
     6781 ttcgcttcga tcaccgtctc gccgggtgtg ccttctaccg tatgtaatat tgcgcccagc
     6841 ggcgattctg aggttccggc accacccagc aaatcaataa atgcatcttc aaaacgcggc
     6901 gtagtttcgt tgatgttgat ttccggcatc ccgtcggcat ggcgaatatc gtctggtgtg
     6961 gcctcttttt tgaggatcag acgtaccgat ttcccctgaa tcatgccgtc gctgacctgt
     7021 ggcagtttca aggcgcgttg caatagtttg cggttgccct cgtgtggact ggtcatcaga
     7081 aaactgcgtc cggccatggt ttgtgtcagg gcttttggtt ccccctgata cagcaactcg
     7141 ccttcgttca tcagtaacac gtcacggcac tgctcggctt cgtcgagata cgaggtactc
     7201 cagaggatta acatcccttc gcctgccagc tcatgcacca tctgccacag ttcgcgccgt
     7261 gagatagggt caacgccgac gccgggttca tcgagcagca acactttcgg ttcgcccacc
     7321 agggtacagg ccagaccgag tttttgtttc atcccaccgg agagcttgcc cgccaggcgt
     7381 ccggtaaacg gcccaagaga cgtaaactcc agcaggcgag caaaagtttg cttacgtgcc
     7441 tcgccggtga cgctgcgcaa atccgcgtac agattgaggt tctccatcac cgtgagatct
     7501 tcatacagac caaatttctg cggcatatag ccgagcacgg cgtgcagcgc gccgtcgttt
     7561 ttgattggat caaagccaat caccgtggca ttgccgctgt cgggtttcag taatcccgcc
     7621 agcatccgca ttagcgtggt tttacctgca ccatccggcc ccaccagccc cgtcacataa
     7681 ccggcgtgaa tggtacaatc gagcggcgcg acggcgggct tgtccatgcc cggaaagcgt
     7741 ttttccaggc cgttcagcgt gataacggca tcattcatgt cctgcctcgt taccgaattg
     7801 caccgtcacc ggcattccct ggcgtaacgc atcatcggcg tcggtcacca caatacgcag
     7861 gcgatagacg aggtcggtac gcagatccgg tgtttcgacg gttttcgggg taaattcagc
     7921 agtcggcgaa acgaaaccaa tctgcccgtg atacggcttg tccgggcgac catcggtata
     7981 aagcagcact ttgcgccccg gctgggcctg gtcaagattc cgttcatcaa cataagcgcg
     8041 cacccacacc ggacgcgtta gtgaaacggt aaacaccgtg ccaccttcat tgaggaccgt
     8101 gcctggctcc accgcgcgcg ttaacagcgt gccatcagac ggggctatca acgttgagtc
     8161 ctgcaaattc aactccgcct gcgccagttg cgcctgcgcc tgttcgaggc tggctttcgc
     8221 ctgggcgatg tcctgttcac ggttaccgga acggtactga cgcaatttat cctgtgctga
     8281 tttcagcgtt gcctgcgcct ggtcgcgcga ggagcgggca ttttccaggt cgtttgccga
     8341 aatggtgcgg cttttccaca acccttgctg gcggttatag aagttctgcg catagtcata
     8401 ggcggcttgc gcctgtttca ccgctgcggc ggcctgagcg atttcttcat cgcgataccc
     8461 ggcaagcatc aggtcatact gcgcctgtgc caccgaaaca cccgctttcg cctgcatcag
     8521 ggcaatctca tacggcttgt gatccagttc gcccagcacc tggcccgctt tgatagcatc
     8581 accttcgtcc accgccagcg attcaacgcg ccccccaaca cggaaactaa gattcaccgt
     8641 acgaatatcc acgttgccat acagcgtcag gccgttatcc tggcggcttt gataccacca
     8701 gtagcctccg gcaaccacgg cggcaagtac cactaccgcc agtccgatca cgacaggttt
     8761 tttcatcact acaaactcct ttgcgataat ccttgcaaaa tcaggtcgat atgacaggtc
     8821 accgtctggt tgatcagttc ggtcttttct tcatcgaacg cggtccagcc ggtacgtaac
     8881 agaattgttt ctttgccaag acggaacgcc agaatctcac caatcaacgc atgggtatga
     8941 aggatcatgc gggtgtcatt ggcgtcgcag ccggtccagg cggcaatcag gcgtgtcagg
     9001 tggctgtgta gcggactaat cacctgttcg tgcaccaggt ggtaggctgc cgtgggagag
     9061 agctgctcac gggagataaa cttgctgagg ttgacggtgt catcctgggt gagcagctta
     9121 atcatgttcc tgcaggcgcg aaggatcagt tcacggatgg cagcccgatc aggctgtggt
     9181 tgggcgaaca agcgttcggc ttcctcggca tgcggacgga actgctcgcc aataaaatcg
     9241 gcaatccact gggcgcaggc gaggtacaaa tcttctttcg aaccgaagta gtaggtgatg
     9301 gcagcgatat tctgcccggc ctgggcggct atctcgcggg tggtggcgtt cattccatat
     9361 tcaccaaact gcgccagtgc ggcagcaatt agctgttttt tcgcctgttc gcccttgatt
     9421 gtcatggcag gattattcat cgcacagtcc attcttaatc aaatgattga ttaagattat
     9481 gactccatag gggagttgtc cagtatggct aagaatttta gctacgccag tcacagggat
     9541 aatttatgcg ctgcgtcaca aaaactgcta cactccgctc cctcatgaca ttgtggtttt
     9601 tgtcatcttc cttttcagta tctccctgaa aactacaccg gtaacggtcg gggcggttcg
     9661 gagtagttat gtctttcgat tctttgggtt taagccctga tatcctgcgc gccgttgccg
     9721 agcagggtta ccgtgaaccc acccctattc agcagcaggc aatccctgcg gtgctggaag
     9781 gccgcgacct gatggctagc gcccagaccg gcactggcaa aacagcgggc tttacgctgc
     9841 cgctgttgca acacctgatc actcgccagc cgcacgccaa agggcgtcgt ccggtacgtg
     9901 cgctcattct taccccgacc cgtgaactgg cggcgcagat tggcgaaaac gtccgtgatt
     9961 acagcaaata cctgaacatt cgttcgctgg tggtgtttgg cggtgtcagc attaacccac
    10021 aaatgatgaa gttgcgcggc ggcgttgatg tgctggtggc aacgccggga cgtttgctgg
    10081 acctggaaca tcagaatgca gtgaagctgg atcaggttga aatcctcgtc ctcgatgaag
    10141 ctgaccgcat gctcgacatg ggctttatcc acgatatccg tcgcgtgtta acaaaactac
    10201 ctgcgaagcg ccagaacctg ttgttctccg cgaccttctc tgacgatatt aaagccctgg
    10261 cggaaaaact gttgcacaac ccgctggaaa tcgaagtggc acgccgcaat accgcgtctg
    10321 atcaggtgac ccagcatgtt cactttgttg ataagaagcg caaacgcgaa ttgctgtcgc
    10381 acatgattgg gaaagggaac tggcagcagg tgctggtgtt tacccgtacc aaacatggcg
    10441 ctaaccatct cgctgaacag cttaataaag atggcatccg tagtgcggcg atccacggta
    10501 ataaatcgca aggtgcgcgt acccgtgcgc tggccgattt taaatcgggc gatattcgtg
    10561 tactggtggc aactgacatc gctgcgcgcg gtctggatat cgaagagttg ccgcacgtcg
    10621 tcaactatga actgccaaac gtaccggaag attatgtcca ccgtatcggg cgtaccggtc
    10681 gtgccgcagc caccggagaa gcgttgtcgt tggtgtgtgt tgatgaacac aaactgctgc
    10741 gtgatatcga aaaactgctg aaaaaagaga tcccgcgcat tgcgattccg ggctatgagc
    10801 cggacccgtc aatcaaagcc gaaccgatcc agaacggtcg ccagcaacgt ggcggcggtg
    10861 gtcgtgggca gggcggtggt cgcggtcaac agcaactacg ccgtggagaa ggtggtgcaa
    10921 aatctgcaag cgcgaagcct gcagaaaaac cgtctcgccg cctcggcgat gccaaaccgg
    10981 taggcgaaca acaacgtcgc cgccgtccgc gtaaacctgc cgctgcgcag taattccatg
    11041 taagggcaaa agagtatcac aggcttttgc ccattattca ggtaacacgg tcagacatgc
    11101 atgacatgtc tgatttt
//
LOCUS       DAPGOF010000162        11077 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.163, whole genome shotgun sequence.
ACCESSION   DAPGOF010000162 DAPGOF010000000
VERSION     DAPGOF010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11077
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.163"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            278..682
                     /gene="sdhC"
                     /locus_tag="RHV13_003775"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             278..682
                     /gene="sdhC"
                     /locus_tag="RHV13_003775"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HDV1243254.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            676..1023
                     /gene="sdhD"
                     /locus_tag="RHV13_003776"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             676..1023
                     /gene="sdhD"
                     /locus_tag="RHV13_003776"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HDV1243255.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            1023..2789
                     /gene="sdhA"
                     /locus_tag="RHV13_003777"
     CDS             1023..2789
                     /gene="sdhA"
                     /locus_tag="RHV13_003777"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HDV1243256.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            2805..3521
                     /gene="sdhB"
                     /locus_tag="RHV13_003778"
     CDS             2805..3521
                     /gene="sdhB"
                     /locus_tag="RHV13_003778"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HDV1243257.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            3822..6623
                     /gene="sucA"
                     /locus_tag="RHV13_003779"
     CDS             3822..6623
                     /gene="sucA"
                     /locus_tag="RHV13_003779"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HDV1243258.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRFESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            6638..7855
                     /gene="odhB"
                     /locus_tag="RHV13_003780"
     CDS             6638..7855
                     /gene="odhB"
                     /locus_tag="RHV13_003780"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HDV1243259.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKSPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            8131..9297
                     /gene="sucC"
                     /locus_tag="RHV13_003781"
     CDS             8131..9297
                     /gene="sucC"
                     /locus_tag="RHV13_003781"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HDV1243260.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            9297..10166
                     /gene="sucD"
                     /locus_tag="RHV13_003782"
     CDS             9297..10166
                     /gene="sucD"
                     /locus_tag="RHV13_003782"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HDV1243261.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(10270..10992)
                     /gene="mngR"
                     /locus_tag="RHV13_003783"
     CDS             complement(10270..10992)
                     /gene="mngR"
                     /locus_tag="RHV13_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HDV1243262.1"
                     /translation="MGRKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLTDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITPQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
BASE COUNT         2625 a         2873 c         3046 g         2533 t
ORIGIN      
        1 tcaggataaa acccgacaaa ctatatgtag gttaattgta atgattttgt gaacggccta
       61 tactgccgcc aggtctccgg aacaccctgc aatcccgagc cacccagcgt tgtaacgtgt
      121 cgttttcgca tctggaagca gtgttttgca tgacgcgcag ttatagaaag gacgctgtct
      181 gacccgcaag cagaccggag gaaggaaatc ccgacgtctc caggtaacag aaagttaacc
      241 tctgtgcccg tagtccccag ggaataataa gaacagcatg tgggcgttat tcatgataag
      301 aaatgtgaaa aaacaaagac ctgttaatct ggacctacag accatccggt tccccatcac
      361 ggcgatagcg tccattctcc atcgcgtttc cggtgtgatc acctttgttg cagtgggcat
      421 cctgctgtgg cttctgggta ccagcctctc ttcccctgaa ggtttcgagc aagcttccgc
      481 gattatgggc agcttcttcg tcaaatttat catgtggggc atccttaccg ctctggcgta
      541 tcacgtagtc gtaggtattc gccacatgat gatggatttt ggctatctgg aagaaacatt
      601 cgaagcgggt aaacgctccg ccaaaatctc ctttgttatt actgtcgtgc tttcacttct
      661 cgcaggagtc ctcgtatggt aagcaacgcc tccgcattag gacgcaatgg cgtacatgat
      721 ttcatcctcg ttcgcgctac cgctatcgtc ctgacgctct acatcattta tatggtcggt
      781 tttttcgcca ccagtggcga actgacatat gaagtctgga tcggtttctt cgcctctgcg
      841 ttcaccaaag tgttcaccct gctggcgctg ttttctatct tgattcatgc ctggatcggc
      901 atgtggcagg tgttgaccga ctacgttaaa ccgctggcct tgcgcctgat gctgcaactg
      961 gtgattgtcg ttgcactggt ggtttacgtg atttatggat tcgttgtggt gtggggtgtg
     1021 tgatgaaatt gccagtcaga gaatttgatg cagttgtgat tggtgccggt ggcgcaggta
     1081 tgcgcgcggc gctgcaaatt tcccagagcg gccagacctg tgcgctgctc tctaaagtct
     1141 tcccgacccg ttcccatacc gtttctgcgc aaggcggcat taccgttgcg ctgggtaata
     1201 cccatgaaga taactgggaa tggcatatgt acgacaccgt aaaagggtcg gactatatcg
     1261 gtgaccagga cgcgattgaa tatatgtgta aaaccgggcc ggaagcgatt ctggaactcg
     1321 aacacatggg cctgccgttc tcgcgtcttg atgatggtcg tatctatcaa cgtccgtttg
     1381 gcggtcagtc gaagaacttc ggcggcgagc aggcggcacg taccgcggcg gctgctgacc
     1441 gtaccggtca cgcactgttg cacacgcttt atcagcagaa cctgaaaaac cacaccacca
     1501 ttttctccga gtggtatgcg ctggatctgg tgaaaaacca ggatggtgcg gtggtgggtt
     1561 gtaccgcact gtgcatcgaa accggtgaag tggtttattt taaagcccgc gctaccgtgc
     1621 tggcgactgg cggagcaggg cgtatttatc agtctaccac taacgcccac attaacactg
     1681 gcgacggtgt cggcatggct atccgcgcag gcgtaccggt gcaggatatg gaaatgtggc
     1741 aattccaccc gaccggcatt gccggtgcgg gcgtactggt caccgaaggt tgccgtggtg
     1801 aaggcggtta tctgctgaac aaacatggcg aacgctttat ggagcgttat gcgccgaacg
     1861 ccaaagacct ggcgggccgt gatgtggtgg cgcgttccat catgatcgaa atccgtgaag
     1921 gtcgcggctg tgatggtccg tgggggccac acgcgaaact gaaactcgat cacctgggta
     1981 aagaagttct cgaatcccgt ctgccgggta tcctggagct ttcccgtacc ttcgctcacg
     2041 ttgatccggt gaaagagccg attccggtta tcccaacctg tcactacatg atgggcggta
     2101 ttccgaccaa agtgaccggt caggcgctga ctgtgaatga gaaaggcgaa gatgtggttg
     2161 ttccggggct gtttgccgtt ggtgaaatcg cttgtgtatc ggtacacggc gctaaccgtc
     2221 tgggcggcaa ctcgctgctg gacctggtgg tctttggtcg cgcagcgggt ctgcatctgc
     2281 aagagtctat cgccgagcag ggcgcactgc gcgatgccag cgagtctgat gttgaagcgt
     2341 ctctggatcg cctgaaccgc tggaacaaca accgtaacgg tgaagatccg gtggcgatcc
     2401 gtaaagcact gcaagaatgt atgcagcata acttctcggt cttccgtgaa ggtgatgcga
     2461 tggcgaaagg gcttgagcag ttgaaagtga tccgcgagcg tctgaaaaat gcccgtctgg
     2521 atgacacttc cagcgagttc aacacccagc gcgttgagtg cctggaactg gataacctga
     2581 tggaaacggc gtatgcaacg gctgtttctg ccaacttccg taccgaaagc cgtggcgcac
     2641 atagccgctt cgacttcccg gatcgtgatg atgaaaactg gctgtgccac tccctgtatc
     2701 tgcctgagtc ggaatccatg acgcgccgaa gcgtcaacat ggaaccgaaa ctgcgcccgg
     2761 cattcccgcc gaagattcgt acttactaat gcggagacag gaaaatgaga ctcgagtttt
     2821 caatttatcg ctataacccg gatgttgatg atgctccgcg tatgcaggat tacaccctgg
     2881 aagcggaaga aggtcgcgac atgatgctgc tggatgcgct tatccagctg aaagagaaag
     2941 atcccagcct gtcgttccgc cgctcctgtc gtgaaggtgt gtgcggttcc gacggtctga
     3001 acatgaacgg caagaatggt ctggcctgta ttaccccgat ttcggcactc aaccagccgg
     3061 gcaagaagat tgtgattcgc ccgctgccag gtttaccggt gatccgcgat ttggtggtag
     3121 acatgggaca attctatgcg caatatgaga aaattaagcc ttacctgttg aataatggac
     3181 aaaatccgcc agctcgcgag catttacaga tgccagagca gcgcgaaaaa ctcgacgggc
     3241 tgtatgaatg tattctctgc gcatgttgtt caacctcttg tccgtctttc tggtggaatc
     3301 ccgataagtt tatcggcccg gcaggcttgt tagcggcata tcgtttcctg atcgatagcc
     3361 gtgataccga gactgacagc cgcctcgacg gtttgagcga tgcattcagc gtattccgct
     3421 gtcacagcat catgaactgc gtcagtgtat gtccgaaggg gctgaacccg acgcgcgcca
     3481 tcggtcatat caagtcgatg ttgttgcaac gtaatgcgta aaccgtaggc ctgataagac
     3541 gcgcaagcgt cgcatcaggc aaccagtgcc ggatgcggcg tgaatgcctt atccggccta
     3601 caagtcatta cccgtaggcc tgataagcgc agcgcatcag gcgtaacaaa gaaatgcagg
     3661 aaatctttaa aaactgcccc tgacactaag acagttttta aaggttcctt cgcgagccac
     3721 tacgtagaca agagctcgca agtgaacccc ggcacgcaca tcactgtgcg tggtagtatc
     3781 cacggcgaag taagcataaa aaagatgctt aagggatcac gatgcagaac agcgctttga
     3841 aagcctggtt ggactcttct tacctctctg gcgcaaacca gagctggata gaacagctct
     3901 atgaagactt cttaaccgat cctgactcgg ttgacgctaa ctggcgttcg acgttccagc
     3961 agttacctgg tacgggagtc aaaccggatc aattccactc tcaaacgcgt gaatatttcc
     4021 gccgcctggc gaaagacgct tcacgttact cttcaacgat ctccgaccct gacaccaatg
     4081 tgaagcaggt taaagtcctg cagctcatta acgcataccg cttccgtggt caccagcatg
     4141 cgaatctcga tccgctggga ctgtggcagc aagataaagt ggccgatctg gatccgtctt
     4201 tccacgatct gaccgaagca gacttccagg agaccttcaa cgtcggttca tttgccagcg
     4261 gcaaagaaac catgaaactc ggcgagctgc tggaagccct caagcaaacc tactgcggcc
     4321 cgattggtgc cgagtatatg cacatcacca gcaccgaaga aaaacgctgg atccaacagc
     4381 gtttcgagtc tggtcgcgcg actttcaata gcgaagagaa aaaacgcttc ttaagcgaac
     4441 tgaccgccgc tgaaggcctt gaacgttacc tcggcgcaaa attccctggc gcaaaacgct
     4501 tctcgctgga aggcggtgac gcgttaatcc cgatgcttaa agagatgatc cgccacgctg
     4561 gcaacagcgg cacccgggaa gtggttctcg ggatggcgca ccgtggtcgt ctgaacgtgc
     4621 tggtgaacgt gctgggtaaa aaaccgcaag acttgttcga cgagtttgcc ggtaaacata
     4681 aagaacacct cggcacgggt gacgtgaaat accacatggg cttctcgtct gacttccaga
     4741 ccgatggcgg cctggtgcac ctggcgctgg cgtttaaccc gtctcacctt gagattgtaa
     4801 gcccggtagt tatcggttct gttcgtgccc gtctggacag acttgatgag ccgagcagca
     4861 acaaagtgct gccaatcacc atccacggtg acgccgcagt gaccgggcag ggcgtggttc
     4921 aggaaactct gaacatgtcg aaagcgcgtg gttatgaagt tggcggtacg gtacgtatcg
     4981 ttatcaacaa ccaggttggt ttcaccacct ctaatccgct ggatgctcgt tctacaccgt
     5041 actgtactga tatcggtaag atggttcagg caccgatttt ccacgttaac gcggatgatc
     5101 cggaagccgt tgcctttgtg acccgtctgg cgctcgattt ccgtaacacc tttaaacgtg
     5161 atgtcttcat cgacctggtg tgctaccgcc gtcacggcca caacgaagcc gacgagccga
     5221 gcgcaaccca gccgctgatg tatcagaaaa tcaaaaaaca tccgacgccg cgcaaaatct
     5281 atgctgacaa gctggagcag gaaaaagtgg cgacgctgga agatgccacc gagatggtta
     5341 acctgtaccg cgatgcgctg gatgctggcg attgcgtagt ggcagagtgg cgtccgatga
     5401 acatgcactc tttcacctgg tcgccgtacc tcaaccacga atgggacgaa gagtacccga
     5461 acaaagttga gatgaagcgc ctgcaggaac tggctaaacg catcagcacg gtgccggaag
     5521 cagttgaaat gcagtctcgc gttgccaaga tttatggcga tcgccaggca atggcagccg
     5581 gtgagaaact gttcgactgg ggcggcgcgg aaaacctcgc ttacgccacg ctggttgatg
     5641 aaggcattcc ggttcgcctg tcgggtgaag actccggtcg cggtacgttc ttccaccgcc
     5701 acgcggtgat ccacaaccag tctaacggtt ccacttacac gccgctgcaa cacatccata
     5761 acggccaggg cgcgttccgt gtctgggact ccgtactttc tgaagaagca gtgctggcgt
     5821 ttgaatacgg ttatgccacc gcagaaccac gcactctgac tatctgggaa gctcagttcg
     5881 gtgacttcgc caacggtgcg caggtggtta tcgaccagtt catctcctct ggcgaacaga
     5941 aatggggccg gatgtgtggt ctggtgatgt tgctgccgca cggttacgaa gggcaggggc
     6001 cggagcactc ctccgcgcgt ctggaacgtt atctgcaact ttgtgctgag caaaacatgc
     6061 aggtgtgcgt accgtctacc ccggcacagg tttaccacat gctgcgtcgt caggcgctgc
     6121 gcgggatgcg tcgtccgctg gtcgtgatgt cgccgaaatc cctgctgcgt catccgctgg
     6181 cggtttccag cctcgaagaa ctggcgaacg gcaccttcct gccagccatc ggtgaaatcg
     6241 acgagcttga tccgaagggc gtgaagcgcg tagtgatgtg ttctggtaag gtttattacg
     6301 acctgctgga acaacgtcgt aagaacaatc aacacgatgt cgccattgtg cgtatcgagc
     6361 aactctaccc gttcccgcat aaagcgatgc aggaagtgtt gcagcagttt gctcacgtca
     6421 aggattttgt ctggtgccag gaagagccgc tcaaccaggg cgcatggtac tgcagccagc
     6481 atcatttccg tgaagtgatt ccgtttgggg cttctctgcg ttatgcaggc cgcccggcct
     6541 ccgcctctcc ggcggtaggg tatatgtccg ttcaccagaa acagcaacaa gatctggtta
     6601 atgacgcgct gaacgtcgaa taaataaagg atacacaatg agtagcgtag atattctggt
     6661 ccctgacctg cctgaatccg tagccgatgc caccgtcgca acctggcata aaaaacccgg
     6721 cgacgcagtc gtacgtgatg aagtgctggt agaaatcgaa actgacaaag tggtactgga
     6781 agtaccggca tcagcagacg gcattctgga tgcggttctg gaagatgaag gtacaacggt
     6841 aacgtctcgt cagatccttg gtcgcctgcg tgaaggcaac agcgccggta aagaaaccag
     6901 cgccaaatct gaagagaaag cgtccactcc ggcgcaacgc cagcaggcgt ctctggaaga
     6961 gcaaaacaac gatgcgttaa gcccggcgat ccgtcgcttg ctggctgaac ataacctcga
     7021 cgccagcgcc attaaaggca ccggtgtggg tggtcgtctg acccgtgaag atgtggaaaa
     7081 acatctggcg aaatccccgg cgaaagagtc tgcaccggca gcggctgctc cggcggcgca
     7141 accggctctg gctgcacgta gtgaaaaacg tgtcccgatg actcgcctgc gtaagcgtgt
     7201 ggcagagcgt ctgctggaag cgaaaaactc caccgccatg ctgaccacgt tcaacgaagt
     7261 caacatgaag ccgattatgg atctgcgtaa gcagtacggt gaagcgtttg aaaaacgcca
     7321 cggcatccgt ctgggcttta tgtccttcta cgtgaaagcg gtggttgaag ccctgaaacg
     7381 ttacccggaa gtgaatgctt ctatcgacgg cgatgacgtg gtttaccaca actatttcga
     7441 cgtcagcatg gcggtttcta cgccgcgcgg cctggtgacg ccggtactgc gtgatgtcga
     7501 taccctcggc atggcagaca tcgagaagaa aatcaaagag ctggcagtta aaggccgtga
     7561 cggcaagctg acggttgaag atctgaccgg tggtaacttc accatcacca acggtggtgt
     7621 gttcggttcc ctgatgtcta cgcctatcat caacccgccg cagagcgcaa ttctgggtat
     7681 gcacgctatc aaagatcgtc cgatggcggt gaatggtcag gttgagatcc tgccgatgat
     7741 gtacctggcg ctgtcctacg atcaccgtct gatcgatggt cgcgaatccg tgggcttcct
     7801 cgtaacgatc aaagagttgc tggaagatcc gacgcgtctg ctgctggacg tgtagtagtt
     7861 taagtttcac ctgcactgta gaccggataa gacattattg ctttctccgg caattgaagc
     7921 ctgatgcgac gcttaacgcg tcttatcagg cctacgggaa gcaccgatgt aggtcggata
     7981 aggcgctcgc gccgcatccg acaagcgatg ccagatgcga cgcttacacg tcttatcagg
     8041 cctacgggtg accgacaata cccggaagcg atacgaaata ttcggtctac ggtttaaaag
     8101 ataacgatta ctgaaggatg gacagaacac atgaacttac atgaatatca ggcaaaacaa
     8161 ctgtttgccc gctatggctt accagcaccg gtgggttatg cctgtactac tccgcgcgaa
     8221 gcagaagaag ccgcttcaaa aatcggtgcc ggtccgtggg tagtgaaatg tcaggttcac
     8281 gctggtggcc gcggtaaagc gggcggtgtg aaagttgtaa acagcaaaga agacatccgt
     8341 gcttttgcag aaaactggct cggcaagcgt ctggtaacgt atcaaacaga tgccaatggc
     8401 caaccggtta accagattct ggttgaagca gcgaccgata tcgctaaaga gctgtatctc
     8461 ggtgccgttg tagaccgtag ttcccgtcgt gtggtcttta tggcctccac cgaaggcggc
     8521 gtggaaatcg aaaaagtggc ggaagaaact ccgcacctga tccataaagt tgcgcttgat
     8581 ccgctgacag gcccgatgcc gtatcaggga cgcgagctgg cgttcaaact gggtctggaa
     8641 ggtaaactgg ttcagcagtt caccaaaatc ttcatgggcc tggcgaccat tttcctggag
     8701 cgtgacctgg cgctgatcga aatcaacccg ctggtcatta ccaaacaggg cgatctgatt
     8761 tgcctcgacg gcaaactggg cgctgatggc aacgcactgt tccgccagcc tgatctgcgc
     8821 gaaatgcgtg accagtcgca ggaagatcca cgtgaagcac aggctgcaca gtgggaactg
     8881 aactacgttg cgctggacgg taacatcggt tgtatggtta acggcgcagg tctggcgatg
     8941 ggtacgatgg acatcgttaa actgcacggc ggcgaaccag ctaacttcct tgacgttggc
     9001 ggcggcgcaa ccaaagaacg tgtaaccgaa gcgttcaaaa tcatcctctc tgacgacaaa
     9061 gtgaaagccg ttctggttaa catcttcggc ggtatcgttc gttgcgacct gatcgctgac
     9121 ggtatcatcg gcgcggtagc ggaagtgggt gttaacgtac cggtcgtggt acgtctggaa
     9181 ggtaacaacg ccgaactcgg cgcgaagaaa ctggctgaca gcggcctgaa tattattgca
     9241 gcaaaaggtc tgacggatgc agctcagcag gttgttgccg cagtggaggg gaaataatgt
     9301 ccattttaat tgataaaaac accaaggtta tctgccaggg ctttactggt agccagggga
     9361 ctttccactc tgaacaggcc attgcctacg gcaccaaaat ggttggcggt gtaactccag
     9421 gtaaaggcgg tactacccac ctcggcctgc cggtgttcaa caccgtgcgt gaagctgttg
     9481 ctgccactgg cgctaccgct tctgttatct atgtgccagc accgttctgc aaagactcca
     9541 ttctggaagc catcgacgca ggcatcaaac tgattatcac catcactgaa ggcatcccga
     9601 cgctggatat gctgactgtg aaagtgaaac tggatgaagc cggcgttcgt atgatcggcc
     9661 cgaactgccc gggcgttatc accccgggtg aatgcaaaat tggtatccaa ccgggtcaca
     9721 ttcacaaacc gggtaaagtg ggtatcgttt cccgttccgg tacactgacc tatgaagcgg
     9781 ttaaacagac cacagattac ggtttcggtc agtcgacctg tgtcggtatc ggcggtgacc
     9841 cgatccctgg ctctaacttt atcgacattc tcgaaatgtt cgaaaaagat ccgcagaccg
     9901 aagcgatcgt gatgatcggt gagatcggcg gtagcgctga agaagaagcg gctgcgtaca
     9961 tcaaagagca cgttaccaag ccagttgtgg gttacatcgc tggtgtgact gcgccgaaag
    10021 gcaaacgtat gggccacgcg ggtgccatca ttgccggtgg taaagggact gcggatgaga
    10081 aattcgctgc tctggaagcc gcaggcgtga aaaccgttcg cagcctggcg gatatcggtg
    10141 aagcactgaa aactgttctg aaataaatat ctgtaataag aaatagccct cgccgcttcc
    10201 ctctacagga atggcgaagg gctgtcggtt tcgacatggt tggccatcgt atgatggcct
    10261 tttttgtgct tatcgcgatg attttcgctg cgctatcagg gtaaatttat agtcatcggt
    10321 attaaaagcg ttgcggctat attcaaacac ccgaccatca actaaatatc cacgcgatac
    10381 tttttcaaga atcggctttg tctggctgat attaagcaga cggctcatct cttcggttgg
    10441 catcagagga atgatttcct gttcgctacg atcgataacc attttcttca cttcttcgat
    10501 aaagtgatat ttcgaatttt ccatgacctg ccaggtgaga tccgggaaca acgcaagcgg
    10561 catccaggtt tcttccagcg ccattggctt ttgcttgcga tagcgcacgc gcttcacatg
    10621 ccacacacga tcctgcgggg tgatttgtag ctgttgctga agaaaatcgt cagccggaat
    10681 cacttcgaat atcagaactt cactgtgtgt atcgacgtga cggtccgtca gtttttcatc
    10741 aaaactggtt aactgaaaaa tatcgtaatt gactcgctct tcttttacgt aagtcccgct
    10801 gccctgaata ctttcgagga tctgctgttc aactaactgg cgcaaagcct ggcgcaccgt
    10861 aacccggctg acgccaaact ctgtttgtag cgctgattca gtgggtaacg catcgccagg
    10921 tttaagctcg ccacgcgcaa tttgctcacg aatgcgatca gcaatctgtc ggtataaggg
    10981 tttccgtccc atgatttagc atctcattaa tacgaattta gccattatgc ccgataaact
    11041 catcctgtaa ataatacaaa taaaatacaa ataattt
//
LOCUS       DAPGOF010000163        11054 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.164, whole genome shotgun sequence.
ACCESSION   DAPGOF010000163 DAPGOF010000000
VERSION     DAPGOF010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11054
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.164"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..817
                     /locus_tag="RHV13_003784"
     CDS             <1..817
                     /locus_tag="RHV13_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV1243263.1"
                     /translation="ALTQSAVFRQVSALEEFLRTPLFSHSKKRIFLNDAGKYYLGIVK
                     ETLNKLERDTNTIMTWQPTVQVIELAVNPTFSTHWLIPNLHEFTKLHPDIIVNIHSLA
                     NNGDFLNREYDAVIMRENFCAPWAEVEYLFDEEILPVCSGSLLAMSDQKLSVAELLTE
                     LPLLHQSTRITGWEEWFALSGVSSPLVNNGPRFDLLSMLIAAVRSNLGVALLPRFAIQ
                     HDLDSGDMVIPCDVPIRTGNRFIMTWQEEKSDSPHLQQFREWLLAKSVVPQEM"
     gene            complement(851..1453)
                     /gene="cobO"
                     /locus_tag="RHV13_003785"
     CDS             complement(851..1453)
                     /gene="cobO"
                     /locus_tag="RHV13_003785"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308783.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HDV1243264.1"
                     /translation="MEARISTERHVQRQQKLKEQVDTRVAAAIEKKGILIVFTGNGKG
                     KSTAAFGTVTRAVGHGKTVGVAQYIKGQWDNGEYNLLQPLGVEFHIMGTGFTWETQNR
                     QADIDAAKEVWSESKRMLADKRYDLVVLDELTYMLAYHYLDTEEVIASLQNRPAQQSV
                     IVTGRGCHSQILKMADTVSEIRPVKHAFDNGIQAQPGIDW"
     gene            complement(1463..3115)
                     /locus_tag="RHV13_003786"
     CDS             complement(1463..3115)
                     /locus_tag="RHV13_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HDV1243265.1"
                     /translation="MSKPFIWQEPFLQNKDGTEYTLISDQHITVTELDGEEVIKIAPE
                     ALTLLARQAFYEASFFLRSAHLQQVASILNDQQASSNDKYVALQLLRNAEVSAKGVLP
                     NCQDTGTATIVASKGQQIWTGGNDAEALSKGIYSTFQENNLRFSQNAPLDMYTEVNTQ
                     TNLPAQIDISAVAGDEYHFLCVNKGGGSANKAALYQETKSLLQPEKLTAFLIEKMKSL
                     GTAACPPYHIAFVVGGLSADQTLKVAKLASTKYYDNLPTSGNEQGQAFRDIELEKALL
                     EASQQFGIGAQFGGKYFAHDIRVMRLPRHGGSCPIAMALSCSADRNIKAKINKHGIWL
                     EKLEHNPGQYIPASLREENHAQHVQLDLNRPLRDVMLDLARLPVGTRVSLSGPIVVAR
                     DIAHAKIKARLDSGESMPEYLKHHIVYYAGPAKTPENMACGSLGPTTGGRMDGYIDTF
                     QAAGGSLVMLSKGNRSQQVTDACHKHGGFNLGSIGGAAALLAQEYVKSLRCLEYPELG
                     MEAVWMMEVENLPAFILVDDKGNNFFSQFEQQHRCASCPAGH"
     gene            complement(3223..4503)
                     /locus_tag="RHV13_003787"
     CDS             complement(3223..4503)
                     /locus_tag="RHV13_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308785.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HDV1243266.1"
                     /translation="MKKISLTTMILLALVLGMIIGVVLNNTASPETAKLYAQEISIFT
                     TIFLRLIKMIIAPLVVSTLVVGIAKMGDAKALGRIFSKTLFLFICASLLSIALGLITV
                     NFFMPGTGINFVAHGAETTGVVASEPFTLKVFISHAFPTSIVDAMAHNEILQIVVFSI
                     FLGCSLTAIGEKGSAIVHALDSLAHAMLKLTGYVMLFAPLTVFAAISALIAERGLAVM
                     VSAGIFMGEFYFTMLLLWVLLIGLAIVYVGPCIRRLTRALSEPALLAFTTSSSEAAFP
                     GTLEKLEQFGVSPKIASFVLPIGYSFNLVGSMAYCSFATVFIAQACNIHLSIGEQITM
                     LLILMLTSKGMAGVPRASMVVIAATLNQFNIPEAGLILLMGVDPFLDMGRSATNVMSN
                     AMGAAMVSRWEGEHFGEGCRGKALKPNESNVALP"
     gene            complement(4664..4981)
                     /locus_tag="RHV13_003788"
     CDS             complement(4664..4981)
                     /locus_tag="RHV13_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HDV1243267.1"
                     /translation="MKQSIVSLAQVIRSKNAGPYELVLDILFKTKENYERVKSSGQLT
                     PELIARLYHVEPDFIHRIVWFDPSNAVKIVMPRDIISGNVGDNDVYGAQQHAPLLNMS
                     FDL"
     gene            complement(4978..6348)
                     /locus_tag="RHV13_003789"
     CDS             complement(4978..6348)
                     /locus_tag="RHV13_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HDV1243268.1"
                     /translation="MARSFKVLSPTAILGYGFPEESFRKAMAESPDLIAVDAGSSDPG
                     PHYLGAGKPFTDRAGVKRDLRYMITAGVQNNIPVVIGTAGGSGAAPHLEWCREIIHEI
                     ALEEQLAFSMALIPADVDKAIVHQALDNGKITALDFVPPLTHDAIDESTYIVAQMGIE
                     PFQRALKEGAQVVLGGRAYDPACFAALPIMQGFDEGLALHCGKILECAAIAATPGSGS
                     DCAMGIIDDNGFTLKTFNPKRKFTETSAAAHTLYEKSDPYFLPGPGGVLNLKGCTFKA
                     VNDGEVYVSGSKHEETPYALKLEGARRVGFRCLTIAGTRDPIMIAGIDKIIDEVKTSV
                     SRNLSLDDDSIRINFHLYGKNGVMGDHEPMQTAGHELGIVLDVVAPTQEIANSVCSLV
                     RSTMLHYGYENRIATAGNLAFPFSPSDIQGGPVYEFSIYHLIEANDALRFDFHIEQVT
                     PEGVQA"
     gene            complement(6352..7593)
                     /locus_tag="RHV13_003790"
     CDS             complement(6352..7593)
                     /locus_tag="RHV13_003790"
                     /EC_number="4.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate ammonia-lyase"
                     /protein_id="HDV1243269.1"
                     /translation="MKIKQALFTAGYSSFYFDDQQAIKNGVGHDGFFYTGEPLTQGFN
                     AVRQAGECVSVQLILENGAVAVGDCAAVQYSGAGGRDPLFLAEHFIPFLNDHIKPLLV
                     GRDVDAFLPNARFFDKLRIDGNLLHTAVRYGLSQALLDATALATGRLKTEVVCDEWQL
                     PRVAESIPLFGQSGDDRYIAVDKMILKGIDVLPHALINNVEEKLGFKGEKLREYVRWL
                     SDRILSKRTSARYHPTLHIDVYGTIGLIFDMDPLRCAQYIASLEKEAQGLPLYIEGPV
                     DAGNKPDQIRLLTAITKELTRLGSGVKIVADEWCNTYQDIVDFTDAASCHMVQIKTPD
                     LGSIHNIVDAVLYCNSHSMEAYQGGTCNETDVSARTCVHVALAARPMRMLVKPGMGFD
                     EGLDIVFNEMNRTIALLQAKD"
     gene            complement(7593..9038)
                     /locus_tag="RHV13_003791"
     CDS             complement(7593..9038)
                     /locus_tag="RHV13_003791"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit E"
                     /protein_id="HDV1243270.1"
                     /translation="MELRNKKLTHDEFMTERQQVLKTWETGKDVENFEDGVKYQQTIP
                     EHKRFSLALLKADKEGKTLSQPRAGVALMDEHIELLKTLQEECDLLPSTIDAYTRLNR
                     YEEAAVGIKKSIEAGTSKLNGLPVVNHGVAACRRLTETLQKPLQIRHGTPDARLLAEI
                     SMSSGFTSYEGGGISYNIPYAKRVTLEKSIHDWQYCDRLMGMYEEHGIRINREPFGPL
                     TGTLIPPFISHSIAIIEGLLALEQGVKSITVGYGQVGSLTQDVAAIQSLRELAHEYFQ
                     SYGYTDYELSTVFHQWMGGFPEDESKAFAIISWGAAVAGMSGATKVITKSPHEAWGIP
                     TAAANIQGLKASRQMLNMVNEQKFPPCPAVELEIELIKSEVRAVLNKVFELGNGDIAR
                     GTVLAFEAGVLDVPFAPAACNAGKILPVRDNTGAIRVLEAGAVPLPKDILDLHHDYVA
                     ERARFEGRQPTFQMVVDDINAVSHSKLIGRP"
     gene            complement(9057..10436)
                     /locus_tag="RHV13_003792"
     CDS             complement(9057..10436)
                     /locus_tag="RHV13_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HDV1243271.1"
                     /translation="MQIVSVDIGSTWTKAALFTREGDALTLVNHVLTPTTTHHLAKGF
                     FSSLNQVLNVDNALPLLNSGEVALKYSSSAKGGLAVAAMGLVPSITLETAKVTAHSAG
                     AKIAQYYAYKLNRRDIQALEETQPDILLFTDGGEESYGLNNARVLAESKLDCAIIYAG
                     NRDIQDEVQEILGHKDLTIVDNVLPDLDHPNPLAARQAICDIFLKRIVKGKGLDIIVD
                     KTGEEPMPTPWTVFELVKAISNVDHSWKEFMLIDMGGATTDVYSACANTLSPDTVLHG
                     VPEPFVKRTVEGDLGMRVSAMVVGESAKELVNVNFAQHPAQLDAFYHYLRHLVAHPDY
                     LPANAEEKYFDTVLAGLCVGYATERHAGTKKEVCTCVGNVDLQMGRDLTTVRKVVGSG
                     GWLSRACQFDMHHWLKYRELNDDGKRILLPTEFEYYRDSRGLLPLLANVARVDPLAAA
                     RTSIQCLTL"
     gene            complement(10436..10885)
                     /locus_tag="RHV13_003793"
     CDS             complement(10436..10885)
                     /locus_tag="RHV13_003793"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit S"
                     /protein_id="HDV1243272.1"
                     /translation="MKKATLVIGVIGADCHAVGNKVLDRVFSNHDFRVINLGVMVSQD
                     EYIDAAIETGADAIIVSSIYGHGDIDCLGMRERCIERGLGDILLYVGGNLVVGKHDFA
                     DVETKFKEMGFDRVFAPSHDLEDVCQLMAHDINQRHDVDTRILEEAI"
BASE COUNT         2621 a         2790 c         2778 g         2865 t
ORIGIN      
        1 agcgttaacc caaagtgcag tattcagaca agtaagtgcg ctggaggaat ttttacgtac
       61 tccgttattt tcacactcta aaaaacgcat ttttcttaat gatgcaggga agtattacct
      121 ggggatagta aaagaaacat taaataagct ggaacgcgat accaatacca ttatgacctg
      181 gcagcctacg gttcaggtta ttgaacttgc cgttaatcct acttttagta cccactggtt
      241 gatcccaaat ttacatgaat tcacaaagtt acatcccgat attattgtta atattcattc
      301 tttagctaat aacggcgatt ttcttaatcg ggagtacgat gctgtcatta tgcgcgaaaa
      361 tttttgcgcg ccctgggcgg aagtagaata tctctttgat gaggagattt tgccggtatg
      421 cagcggcagt ttgcttgcaa tgtccgatca aaaactgtca gtggcagaac tgcttactga
      481 gttaccactt ctgcatcaaa gcacccgtat tactggctgg gaagagtggt ttgctttatc
      541 tggcgttagc agtccgctgg tcaacaacgg accacgtttc gatttgcttt caatgctgat
      601 tgccgccgtg cgctctaatc tcggcgtggc gctgttacca cggttcgcta ttcaacatga
      661 tttggatagt ggcgatatgg tgatcccctg tgatgtgcct attcgtaccg gtaatcgttt
      721 tattatgacg tggcaggaag agaagtctga ctcaccgcac ttgcagcaat ttcgtgaatg
      781 gttgctggcg aaatcggtcg taccgcagga gatgtaataa ctgcgcctgg caggatgcca
      841 ggcgcagtta ttaccagtcg atacccggct gggcctgtat gccgttatca aaagcatgtt
      901 tgacggggcg gatttcgctg accgtatctg ccatttttaa tatctgactg tggcaaccgc
      961 gtccggtgac aatgacactt tgttgtgccg ggcggttttg cagagaagcg atcacttctt
     1021 cggtatccag ataatggtaa gccagcatat aggtcagctc atcaagcacc actaagtcgt
     1081 aacgcttgtc agccagcatc cgtttgcttt ccgaccagac ctctttcgcc gcgtcgatat
     1141 ccgcttgccg gttctgtgtc tcccaggtaa aaccggttcc cataatatga aactcaaccc
     1201 ccagcggctg gagaaggttg tattcgccgt tgtcccattg ccccttgatg tactgcgcaa
     1261 cccccaccgt tttgccatga cccaccgcgc gcgtcacagt accaaacgcg gcagtggatt
     1321 tccctttgcc gttgccggtg aaaacaatca agatcccttt tttctctatc gccgccgcga
     1381 cgcgcgtatc gacctgttcc ttcaatttct gttggcgctg cacatgccgt tcagttgaaa
     1441 ttcgcgcttc cattcatttt ccttaatgtc ccgcagggca actggcgcag cggtgttgct
     1501 gctcaaactg actaaagaaa ttattgcctt tatcatctac caggatgaat gcgggcagat
     1561 tttccacttc catcatccac accgcttcca tacccagctc cggatattcc aggcaacgca
     1621 gacttttgac atattcttgc gccagtaacg cagctgcacc gccaatactt cccagattga
     1681 agccgccatg tttgtgacag gcatcggtga cctgctggct gcggttacct ttcgataaca
     1741 tcaccagact gccgccagcc gcctggaagg tatcgatata accgtccatt cgaccgcccg
     1801 tggttggacc cagtgaaccg caggccatgt tctccggtgt tttcgccggg ccagcgtaat
     1861 agacaatatg atgcttcagg tactccggca tcgattcgcc gctatccagc cgcgctttta
     1921 ttttggcgtg ggcgatatcg cgcgccacta cgatgggacc acttaacgac acgcgggtgc
     1981 ctaccggtaa tctggcgaga tccagcatca cgtcacgcaa cggacggttt agatccagtt
     2041 gtacatgttg cgcgtgattt tcttcccgca atgaggctgg aatatattgc cccggattgt
     2101 gttcgagttt ttccagccag ataccgtgtt tgttaatttt tgctttgata ttgcgatcgg
     2161 cagaacagga gagcgccata gctatcgggc aagagccacc gtgacgcggc agacgcatga
     2221 cgcgaatatc atgagcgaaa tatttccccc caaattgtgc accaatacca aattgctgac
     2281 tggcctcgag caacgctttt tccagctcaa tatcacggaa tgcttgtccc tgctcgtttc
     2341 cgctggtggg caaattgtcg taatatttcg ttgaggccag cttggcaact tttaaggttt
     2401 gatcggctga aagccctccg acgacaaagg cgatgtggta aggcgggcag gcagcggtgc
     2461 ccagcgattt cattttttcg attaaaaatg cggtcagttt ttctggttgc aataaggatt
     2521 tggtttcctg ataaagcgcg gctttattgg cggagccgcc ccctttatta acacagagaa
     2581 aatggtactc atcaccagcg acagcgctga tatcaatctg cgccgggaga ttggtttgcg
     2641 tattgacttc ggtatacata tccagcggcg cattttgtga aaagcgcaga ttattttcct
     2701 ggaaagtact gtaaatgcct ttgcttaagg cttcagcgtc gttacctccg gtccatatct
     2761 gttgcccttt actggcaaca atagttgcgg tgccggtatc ctgacagttg ggtaataccc
     2821 cttttgctga gacttcagca ttacgtaata actgcaacgc aacgtattta tcattactac
     2881 tggcctgctg atcgttaaga atgctggcga cctgttgcaa atgagcggaa cgtaagaaaa
     2941 aagaggcttc ataaaatgcc tgccgggcaa gcagcgttaa tgcttcagga gcgattttta
     3001 tcacttcttc gccgtctaat tccgttactg taatatgctg atcgctaatt aatgtatatt
     3061 ctgtaccgtc tttattttgt agaaacggtt cttgccagat aaatggtttg gacatatttc
     3121 acttttcacc ttaaacgtta gtgttactta attgccgtct aacttcatga tccggcttat
     3181 aaaaatgcgt agtcattggc ttctgattgc ttaagatccc cctcagggca gagcaacgtt
     3241 cgattcattg ggttttaatg ctttaccccg acagccctcg ccgaaatgtt cgccttccca
     3301 ccgactcacc atcgcagcgc ccattgcgtt gctcatgacg tttgtcgcgg aacgccccat
     3361 atcaaggaac ggatcaacgc ccatcagcaa gatcagcccc gcttccggaa tattgaactg
     3421 gttgagcgtg gcggcgataa ccaccattga ggcgcgtggt acgccagcca ttcctttcga
     3481 ggtcaacatc aggatcaaca gcatggtgat ttgttcaccg atggataaat ggatattgca
     3541 ggcctgggcg atgaaaactg tggcgaagga gcagtaggcc attgatccaa cgagattaaa
     3601 tgagtagcca atgggtaaga caaagctggc aattttgggg gaaacgccaa attgctccag
     3661 tttttcaagc gttcccggaa aagccgcttc agaactggat gtggtaaatg ccagcagggc
     3721 gggttccgaa agggcgcggg tcaggcgtct gatgcagggg ccgacataaa cgatggccag
     3781 accgataagc agcacccaaa gtaataacat ggtgaaataa aattcaccca taaagatccc
     3841 ggcgctcacc ataacggcca gtcctcgttc agcaatcaat gctgaaatag cggcgaatac
     3901 ggtcagggga gcgaagagca tgacgtagcc agtgagcttt aacatggcat gtgccagcga
     3961 atctaaggcg tgaacgatgg cgctgccttt ctcgccaatc gccgtcaggc tacagccgag
     4021 gaaaattgag aacaccacga tttgcaaaat ttcattgtgc gccatggcat cgacaatgct
     4081 ggtggggaaa gcatgcgaaa taaatacttt tagcgtaaag ggttctgacg cgaccactcc
     4141 tgtggtttcg gcaccgtgtg caacaaaatt aattcctgtg cctggcatga agaaatttac
     4201 cgttatcaag cctaaggcaa ttgacagcaa tgaggcgcaa ataaataaaa aaagtgtttt
     4261 agaaaaaata cgaccaaggg ctttggcatc gcccatttta gcaataccta ccaccagggt
     4321 agagaccact aacggagcga taatcatttt tatcagtcgt aagaaaatcg tcgtgaatat
     4381 cgatatttct tgcgcataga gttttgcggt ttccggtgaa gcagtgttat tgagcactac
     4441 gccgataatc attccaagta ccagcgccaa aagaatcatc gtggttaaac ttattttctt
     4501 cattttttcc tccatcaggt gcacggcgat aaatcagaat caggagaagt agatgcctga
     4561 atagagattc aggcatctac ctgctattac aaccttacaa atagctgaag tgttgaactg
     4621 caataagtga cgggatgtca gttggctaaa agtattcgga tttttacaaa tcgaaactca
     4681 tatttaataa aggtgcatgt tgctgtgcac catagacatc attgtcacct acattcccgg
     4741 agataatatc tcgtggcatg acgattttta ctgcgttaga aggatcaaac cagacaatac
     4801 gatgaataaa gtcaggttca acatgatata agcgcgcaat cagttctggg gttaattgcc
     4861 ctgatgattt aactcgctca tagttttctt tcgttttaaa taaaatatct aaaaccagtt
     4921 cgtaaggtcc ggcatttttc gaacgaatga cttgcgccag tgagacaatt gactgtttca
     4981 tgcctgaact ccttctggcg tcacctgttc aatatggaaa tcaaaacgca gagcgtcgtt
     5041 ggcttcaatc aggtgatata tggaaaattc gtataccggc ccgccctgga tatcagaagg
     5101 ggagaacggg aacgccagat tgcctgcggt agcgatgcgg ttttcatagc cgtagtgcag
     5161 catggtagag cgcaccagcg agcaaacgct attggcaatt tcctgggtcg gtgcaactac
     5221 atcaaggaca atccccagct catgcccggc agtttgcatc ggttcatggt ctcccatcac
     5281 cccgtttttg ccgtacaggt ggaaattgat gcgaatgctg tcatcgtcga gcgacaggtt
     5341 acgcgaaacg ctggttttga cttcatcgat gattttatcg atcccggcga tcataatcgg
     5401 gtcgcgcgtt ccggcgatgg tcagacagcg gaagcccacc cgtcgtgcac cttccagttt
     5461 cagggcatac ggcgtttctt catgcttaga accgctgacg tagacttcgc cgtcattgac
     5521 tgctttaaat gtgcagcctt tcaggttcaa cacgccgcca ggtcctggca ggaagtaggg
     5581 atcggatttc tcatacagtg tgtgtgcagc tgctgacgtt tcggtaaatt tacgcttcgg
     5641 attaaatgtc ttcagcgtaa agccgttgtc atcaatgatg cccatcgcac agtcagagcc
     5701 tgagccgggc gttgcggcga ttgccgcgca ttcaaggatt ttcccacaat gcagcgccag
     5761 accttcatca aagccttgca tgatcggtag cgcagcaaag caggccgggt cgtaagcgcg
     5821 cccgcccagc accacttgcg cgccttcttt cagcgcccgc tggaagggtt cgatgcccat
     5881 ttgcgcaacg atatacgtac tttcgtcaat cgcgtcgtgg gttaacggcg ggacaaaatc
     5941 cagcgccgtg attttgccgt tatccagcgc ctggtggacg atggctttgt caacgtctgc
     6001 cgggatcaac gccatggaga acgcgagttg ctcttccagg gcaatctcat gaattatttc
     6061 tcggcaccac tccaggtgtg gtgcggcacc agaaccgcca gccgttccga tcaccaccgg
     6121 tatattattt tgcacgcctg cggtgatcat atagcgcaga tcgcgcttga cccctgcgcg
     6181 atcggtaaac ggtttgcctg ccccgaggta gtgggggcct ggatcggaag agcctgcatc
     6241 aacggcgatc agatcgggcg attctgccat cgctttgcgg aagctctctt ccgggaatcc
     6301 atacccgagg attgccgttg gcgataagac tttaaaagaa cgagccattt gttaatcctt
     6361 agcctgcaac agcgcgatag tacgattcat ttcgttgaag acgatatcga ggccttcgtc
     6421 aaagcccatc cctggtttta ccagcatacg catagggcga gcggcaaggg cgacgtggac
     6481 acaggtgcgg gcactgacat cagtttcatt gcaggtgccg ccctggtacg cttccatgct
     6541 gtggctattg cagtaaagaa ccgcatcgac gatgttgtga atactgccca gatccggggt
     6601 tttgatttgc accatgtgac aactggcggc atcagtgaaa tcaacaatat cctggtaggt
     6661 gttacaccat tcatcggcca caattttcac gccggaaccg aggcgcgtca gctctttggt
     6721 aatcgcggtc agcaggcgaa tttggtcggg cttgttaccg gcatcgaccg gcccttcaat
     6781 gtagagcggc aggccttgcg cttctttttc caggctggcg atgtattgcg cacagcgaag
     6841 cggatccata tcgaagatca gaccgatagt gccgtataca tcgatgtgca gggtagggtg
     6901 gtagcgtgcg ctggtgcgct tgcttagaat gcgatccgac aaccagcgga catattcgcg
     6961 cagtttttca cctttaaagc ccagcttctc ttcgacgtta ttaatcagcg catggggcag
     7021 cacgtcgatg cccttaagga tcatcttatc gacggcgata tatcgatcgt cgccgctctg
     7081 accaaataat ggaatggatt ccgccacgcg tggcaactgc cattcatcac agaccacttc
     7141 agttttcaga cggccggttg ccagcgcggt agcatcaagc agcgcctgtg ataatccgta
     7201 gcgcacggcg gtatgcagca agttgccgtc aatacgcaat ttgtcgaaga aacgggcatt
     7261 cggcaggaaa gcatccacat cgcggcctac cagtaatggc ttgatatggt cgttgaggaa
     7321 cggaataaag tgctctgcga ggaacagtgg atcgcgacca cctgccccgg aatactgtac
     7381 ggcagcacag tcacctacag cgaccgcgcc gttttccaga atcaactgta ccgatacgca
     7441 ctccccggcc tgacgtacgg cgttaaaccc ctgtgttaat ggctccccgg tataaaaaaa
     7501 gccgtcgtga cccactccgt tttttatcgc ctgctggtca tcgaaataga atgaggaata
     7561 gccagcggtg aacagggcct gttttatttt cattatggtc ttcctattaa tttactatgg
     7621 gatacagcat tgatgtcatc tacaaccatc tggaatgtcg gttggcgtcc ttcaaaacgt
     7681 gcgcgttccg caacgtaatc gtggtggagg tcgagaatat cttttggcaa tggaacggct
     7741 ccggcttcca gtacgcgtat cgcgccggtg ttgtcacgga ccggaagaat tttgcccgcg
     7801 ttgcaggcgg ctggcgcaaa cggcacatcc agtacgcccg cttcaaatgc cagcaccgta
     7861 ccgcgcgcaa tatcaccatt acccagttcg aacactttat tcagtaccgc gcgaacctcg
     7921 ctcttaatca gttcaatttc cagttcaacc gcagggcacg gcgggaattt ctgctcgttg
     7981 accatgttga gcatctggcg ggaggctttc agcccctgaa tgttggcagc ggcagtcggg
     8041 atgccccagg cttcatgcgg acttttggtg atgactttgg tggcaccaga catccccgcg
     8101 acggctgcgc cccatgagat aatagcgaac gctttcgatt cgtcttccgg aaagccgccc
     8161 atccactggt gaaacacggt acttaattca tagtcggtat agccatagct ctgaaaatat
     8221 tcgtgtgcaa gttcacgcag agactggatc gccgcaacat cctgcgtcag gctgccgacc
     8281 tgtccataac caacggtgat agatttcaca ccctgttcca gcgccagcaa gccttcgatt
     8341 atcgcgatag agtgcgagat aaacggcggg atcagcgtac ctgttagtgg gccaaacggt
     8401 tcgcggttga tgcggatccc gtgttcttca tacatcccca ttaaacggtc gcagtactgc
     8461 cagtcgtgga tcgatttttc cagcgttacg cgtttggcat acggaatgtt gtaggaaatg
     8521 ccgccgcctt cgtaactggt aaagccgctg gacatcgaga tttcagccag cagacgcgca
     8581 tctggcgtgc cgtggcggat ctgtagcggt ttttgcaggg tttcggtgag gcgacggcaa
     8641 gctgcgacgc cgtggttaac taccggtaaa ccgttcagtt tagaggttcc ggcttcgatg
     8701 gattttttaa ttcctacagc cgcttcttcg taccgattca gacgggtata tgcatcgata
     8761 gtgctcggca gcagatcgca ctcttcttgt agtgttttca gtagttcgat gtgttcgtcc
     8821 atcagcgcca cacctgcgcg cggttggcta agggttttac cttccttgtc tgctttcagc
     8881 agcgcaagag agaagcgttt atgctcagga atggtttgtt ggtatttaac gccatcttca
     8941 aagttttcaa catccttgcc ggtttcccag gttttcagta cctgttgccg ctcggtcatg
     9001 aattcgtcat gggttaattt cttatttcga agttccattg attgcagcct taattgttat
     9061 agggttaagc attgaatact ggtgcgcgcg gcggccagcg gatcgactct ggcgacgttt
     9121 gccagcagtg gtaatagccc ccgggaatcg cggtaatatt caaattcggt cggtaagaga
     9181 atgcgtttac cgtcgtcgtt taactcgcgg tatttcaacc agtgatgcat atcgaactga
     9241 caggcacgcg agagccatcc gccggatccg acgactttac gcacggttgt caggtcacgt
     9301 cccatctgta aatcgacatt gccgacgcag gtgcagacct cttttttggt gcccgcatgt
     9361 ctttcggtgg cataacccac acacagtccg gccaggacgg tatcgaaata tttctcttct
     9421 gcatttgcgg gtaagtagtc gggatgagcg actagatgac ggaggtaatg ataaaaagcg
     9481 tccagctgtg cggggtgctg agcaaaattc acattgacta actctttcgc actttcacca
     9541 accaccattg ccgagacgcg cattcccaga tcgccttcca ccgtccgttt tacgaagggt
     9601 tccggtacgc cgtgtaaaac ggtgtcgggc gagagcgtgt tggcacaggc ggaatagaca
     9661 tcggtggttg cgccacccat gtcgatcagc atgaactctt tccagctgtg atcgacatta
     9721 ctgattgcct taaccagttc aaagactgtc cacggtgtcg gcattggttc ttcgcctgtt
     9781 ttatcaacaa tgatatccag ccctttccct ttaacgatgc gtttaaggaa tatgtcgcag
     9841 atagcctggc gtgcggctaa agggttcgga tggtcaagat cgggcagaac gttgtcgacg
     9901 atggtcaggt ctttatgccc gaggatctcc tgcacctcgt cctgaatatc ccggtttccg
     9961 gcataaataa tggcgcagtc gagtttggat tctgccagca cacgggcgtt attcagtccg
    10021 tagctttctt ctccgccgtc ggtgaacaag agaatatcgg gctgcgtttc ttccagtgcc
    10081 tggatatcac ggcggttcag cttgtaggcg taatattgcg caattttagc ccccgcagag
    10141 tgagcggtta ctttcgcggt ttccagggtg atagaaggca ccaatcccat tgcggcaacc
    10201 gccagcccac ctttggcaga ggaggaatac ttcaatgcaa cctcaccgct gtttaacagg
    10261 gggagggcat tatctacgtt taacacctgg ttcaggctgg agaaaaaccc cttcgccaga
    10321 tgatgggtgg tggttggggt cagtacgtgg ttaaccaacg ttaacgcgtc cccctcccgg
    10381 gtgaagaggg ctgctttggt ccacgtcgat ccaatatcga cagagacgat ttgcatcaga
    10441 tagcctcttc aagaatacga gtatcaacgt catgacgttg attgatatcg tgggccatca
    10501 gctgacaaac atcttctaag tcatgacttg gggcaaagac gcgatcaaat cccatctctt
    10561 tgaacttggt ttccacgtca gcgaagtcat gttttccgac caccaggtta ccgcccacat
    10621 agaggaggat gtcacccagt ccacgctcga tgcagcgttc gcgcatgccc agacagtcga
    10681 tatcgccatg cccatagata gaggagacga taatcgcgtc agcgccagtt tcgatggcag
    10741 catcaatata ttcgtcctgg ctgaccatta cgcccagatt aatgactctg aagtcgtggt
    10801 tgctaaaaac gcggtccaga actttattgc ctactgcatg gcagtccgcg ccaataacgc
    10861 caatcacaag tgttgctttc ttcatgggta atcctttgta agacagagag taaaataagt
    10921 tacacacgtc gtatttatgg ccgttcataa cggcacaggt ttaatatcac aggagtgtct
    10981 ttatctgacc gtaatgttgc aattgatcta ttttcaaaat gtctttcggg aaatgattgt
    11041 ttaaagtgtg tttt
//
LOCUS       DAPGOF010000164        11049 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.165, whole genome shotgun sequence.
ACCESSION   DAPGOF010000164 DAPGOF010000000
VERSION     DAPGOF010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11049
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.165"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            306..1031
                     /locus_tag="RHV13_003794"
     CDS             306..1031
                     /locus_tag="RHV13_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDV1243273.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMNETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            989..1666
                     /gene="bglJ"
                     /locus_tag="RHV13_003795"
     CDS             989..1666
                     /gene="bglJ"
                     /locus_tag="RHV13_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HDV1243274.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1704..2492)
                     /gene="fhuF"
                     /locus_tag="RHV13_003796"
     CDS             complement(1704..2492)
                     /gene="fhuF"
                     /locus_tag="RHV13_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HDV1243275.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDDPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2633..2869
                     /locus_tag="RHV13_003797"
     CDS             2633..2869
                     /locus_tag="RHV13_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HDV1243276.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIIILMGLVLTP
                     MMLYHKQLRHYVLLPSCLALIAGIMLMIMNLNQG"
     gene            complement(2908..2994)
                     /locus_tag="RHV13_003798"
     tRNA            complement(2908..2994)
                     /locus_tag="RHV13_003798"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2958..2960),aa:Leu,seq:cag)
     gene            complement(3262..4293)
                     /gene="rsmC"
                     /locus_tag="RHV13_003799"
     CDS             complement(3262..4293)
                     /gene="rsmC"
                     /locus_tag="RHV13_003799"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HDV1243277.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            4396..4809
                     /gene="holD"
                     /locus_tag="RHV13_003800"
     CDS             4396..4809
                     /gene="holD"
                     /locus_tag="RHV13_003800"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HDV1243278.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            4778..5224
                     /gene="rimI"
                     /locus_tag="RHV13_003801"
     CDS             4778..5224
                     /gene="rimI"
                     /locus_tag="RHV13_003801"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDV1243279.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            5239..5916
                     /gene="yjjG"
                     /locus_tag="RHV13_003802"
     CDS             5239..5916
                     /gene="yjjG"
                     /locus_tag="RHV13_003802"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HDV1243280.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            6007..7596
                     /gene="prfC"
                     /locus_tag="RHV13_003803"
     CDS             6007..7596
                     /gene="prfC"
                     /locus_tag="RHV13_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDV1243281.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            7989..8594
                     /gene="osmY"
                     /locus_tag="RHV13_003804"
     CDS             7989..8594
                     /gene="osmY"
                     /locus_tag="RHV13_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HDV1243282.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            8721..8882
                     /locus_tag="RHV13_003805"
     CDS             8721..8882
                     /locus_tag="RHV13_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HDV1243283.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            9004..10077
                     /locus_tag="RHV13_003806"
     CDS             9004..10077
                     /locus_tag="RHV13_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HDV1243284.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            10074..10856
                     /locus_tag="RHV13_003807"
     CDS             10074..10856
                     /locus_tag="RHV13_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDV1243285.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVLALAENYQPLYAALGLHPGMLEKHSYVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDSQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLSRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYAVFSVSG"
BASE COUNT         2718 a         2700 c         2938 g         2693 t
ORIGIN      
        1 cattctgcgg gaagggatat catttaatta taaattaaat gaatgtgatt tctgtttttc
       61 attaagtatt cctaacaact taatgtagat attttaaatg tctccaggct atttcaccag
      121 gttatcattt gatgctaacc ttctgacaca tgcagtggag ttgttgtgca gcaggagtat
      181 gctgatatga aagtgaatgc taaggataat ttattcgctt aatctattaa tttgctggaa
      241 acatttaagg aatgaatatc ctgaataaac catcatgatt tttagacagt ggatgtggag
      301 gaaatatgtt gccaggatgc tgcaaaaatg gaattgttat cagtaaaata cctgttatgc
      361 aagcagggtt aaaagaggtc atgaggactc acttccctga atatgaaata atatccagcg
      421 cctctgcgga ggaccttacc ttattacaat tacgtcgttc cggattagtc attgctgatt
      481 tagccggtga aagtgaagat cctcgttctg tttgtgaaca ttattattct ttaatctcac
      541 aatatcggga aattcactgg gttttcatgg tgtcacgctc ctggtattcc caggcagtag
      601 aattgctcat gtgccctacg gcgacgttat tgtctgatgt tgaacccatt gagaatctgg
      661 tcaagaccgt acgttccggc aatacgcacg cagagcgtat cagcgccatg ctgacctctc
      721 cggcaatgaa tgaaactcat gattttagct atcgctccgt cattctcact ctttcagagc
      781 gcaaggtact gcggctatta ggtaaaggat ggggcatcaa ccagatagct tcgttgctta
      841 agaaaagtaa taaaactatc agcgcccaaa aaaacagtgc gatgcgtcga ctggcaattc
      901 acagcaacgc tgaaatgtat gcatggataa atagcgcgca gggtgcaaga gaacttaact
      961 tgccttctgt ttatggagat gccgcagaat ggaacacagc cgaattaaga agagaaatgt
     1021 cgcactcata gaaaaatgcg tcatgagcag tatcggtatt gagagtttat tcagaaagtt
     1081 tgcgggtaac ccttataagc tccataccta taccagtcag gagtcatttc aggatgccat
     1141 gtcgcggatc tcatttgcgg cggtcatttt ttctttttct gccatgagaa gtgagcgcag
     1201 agagggatta tcttgcctga ctgaactggc gattaagttt ccgcgtaccc ggcgtttagt
     1261 tattgcggat gatgatattg aagctcggct gattggttca ttgtcgccat caccgctgga
     1321 cggtgtatta agtaaagcgt caacgctgga gatttttcat caggaactct ttttgtcatt
     1381 aaatggtgta cgtcaggcga ccgaccgact gaacaatcag tggtacatta accaaagccg
     1441 gacgctaagc ccgacggaga gagaaatatt gcgctttatg tcgcgtggct actcaatgac
     1501 acaaattgcc gagcagctta aacgcaatat caaaacgatc cgtgcacata aatttaatgt
     1561 gatgtcgaaa ctgggcgtca gttctgatgc agggttgttg gaggccgcag atattctgtt
     1621 atgtatgcgg cattgcgaag caagtaatgt gttgcatccc tattaatccg catgatgccg
     1681 ggtttacttc cccggcagtg ctttcatttc agcgtacaat cgccacattg ctgcacatcc
     1741 ggtaagcgat aacgctggca gcaggtccgg cgcaccagca ggccgtcgcg cagtaccacg
     1801 gtacgccaga gtggattatc ttcaccgttc gtgagcgttt tctcaaaaaa gagggcatgg
     1861 cgcagcgatt caacagtagc ctcgccgagc agttgcttca tctcagtgag ataccagttg
     1921 atcaaataac cggtattact ccagataagt ttgccgttga tctctccggt cgcttctagt
     1981 gcctgcacaa ccggaaccag cgcctggctg attaacgttt ccattcgctg ctgcggcgaa
     2041 tgtggtgttg cgtttttatc ttcacacaca tcgacccaga aacaggcgac gcgtccggtt
     2101 tcgtgaaact cagcatggaa atgttcaggc gacacatcta atgctttttc ctgcgtcagt
     2161 agcgccagca ttaatggtgg caccatcagg ccgatatacc attgtgccca tagtgagatc
     2221 agcggtttgt tctcgcggat catcgttggt tggttgcgat agatatgatc ggaatagacc
     2281 gcgagtagag aacttagcgc attcggtgat gaccattgcg ccagcgtcat ggcgttaagc
     2341 ggagcggggt catccaggcg gataaactcc agcaaatgtt cacgatgttt ggcgatcgtc
     2401 gcacgtacgg cttgtgcaag cccagcatcc tgtggctgga gatgcgttcg ccagataaca
     2461 tcttcataga gcggtgcgga acgataggcc ataatcggga tagtaatcta aatgataatg
     2521 attgctaatc ctagcgatag gtttacccga tagcaaggga tttctctggc ttgcaaatga
     2581 taaaaattat catatgatat tggttatcat tatcaatgaa agagatgaaa ccatgttgca
     2641 acgtacgctg ggcagtggct ggggagtgtt gctgccggga ttgctgattg cagggctgat
     2701 gtatgcggat ttatcgccgg atcaatggcg gattatcatt ctgatgggat tagtattgac
     2761 gccgatgatg ctgtatcaca aacagttgcg gcattacgtt ttgctaccat cgtgcctggc
     2821 gcttattgct ggcatcatgc tgatgataat gaatttgaat cagggatgat aaaacaggga
     2881 agaaacaaga aaggaagtaa agataattgg tgcgaggggg gggacttgaa cccccacgtc
     2941 cgtaaggaca ctaacacctg aagctagcgc gtctaccaat tccgccacct tcgcatacca
     3001 tcaattctta aaaagaattg ctaccacgga ggcgcattct agtggttttc agcttttcgt
     3061 caatagttaa ttatcgccgg aggtgtaatt gctggaaaaa tgtccatcag gaaactagcg
     3121 tgcaggtttg gtaggcatgc gggggcagat gccagatgcg acgctggcgc gtcttatctg
     3181 gcctacgaag ggctaacgtg caggttttgt aggtcggata aggtgttcac gccgcatccg
     3241 acacagtatt cggcgggata cttaaccttt cttcgcctga cgggtcataa tggcgcgata
     3301 caccttgaag cgcccggttt gcgcgatcac ttcgtggaag ccaaatgtct catccagcac
     3361 gtccgggtaa ggcaggaagg cgttggctac aattcgcagc tcgccgccac tattaagatg
     3421 acgcaccgcg ccgcgaatca gcgtttgcgc cgcatcgagg ctggtttgca tcccatcgtg
     3481 gaacggcggg ttggagatga tcatatcaaa acgacctttc acctcggaaa agacgttgct
     3541 ggcaaagact tcaccttcaa cgccgttggc ggcaagtgtt gcgcggctgg cttctaccgc
     3601 tggcgcagag acatcgcaca gagtgagacg aattttcggc gaatggcgcg caaaggcaac
     3661 tgaaagcaca cccgcgccac agccgacatc cagcacttta cctttcgtgt gcggagttaa
     3721 cgtcgagagc agcaactggc taccgacatc cagaccgtcg cggctaaaca cgccaggcag
     3781 cgttttgacc gtcaggccat cgacgctgta ttcgccccag aatttatccg catcaaatac
     3841 tggctgtttt tccagacgac caaaatagag gccacagcga cgagcgctgt cgactttatt
     3901 caacggcgca taatctgcca gcatctgctc ggcgctgcgc acgccgctgc ggttctcgcc
     3961 aacgacaaaa atatctgtcc ccactggcag cagagaaagt aaattcatca actggaactg
     4021 ggcttccggt ttgttcttcg gccagtagta aatcagcgta tcgcaatctg cgacgtcatc
     4081 caccgtggcg accagactga aacgggcgtt atcccccatc tggcggctta atacctgcca
     4141 gtggtggaat tgctgggtat gagcacggct ggccgcggta tctaaacgcg cgggcaggtc
     4201 atcctgtaag tctccggcaa acagaatacg gctttgttcg aaatcatcac tgtgacgcag
     4261 caagacttca cttgccgggg taaatgcaga catggaatgc tcctcaattg atactggcgg
     4321 cgattatagc catatgttgg cgcggtatcg acgaatttgc tatatttgcg cccctgacaa
     4381 caggagcgat tcgctatgac atcccgacga gactggcagt tacagcaact gggcattacc
     4441 cagtggtcgc tgcgtcgccc tggcgcgttg cagggggaga ttgccattgc gatcccggca
     4501 cacgtccgtc tggtgatggt ggcaaacgat cttcccgccc tgaccgatcc tttagtgagc
     4561 gatgttctgc gcgcattaac cgtcagcccc gatcaagtgc tgcaactgac gccagaaaaa
     4621 atcgcgatgc tgccgcaagg cagtcgctgc aacagctggc ggttgggtac tgacgaaccg
     4681 ctatcactgg aaggcgctca ggtggcatca ccggcgctca ccgaattacg ggcaaaccca
     4741 acggcacgcg ccgcgttatg gcaacagatt tgcacatatg aacacgattt cttccctcga
     4801 aacgactgat ttaccagcgg cttaccacat tgaacaacgc gcccacgcct ttccgtggag
     4861 tgaaaaaacc tttgccagca accagggcga gcgttatctc aactttcagt taacgcaaaa
     4921 cggcaaaatg gcggcgtttg cgattacgca agtggtgctg gatgaagcta cattgttcaa
     4981 tattgcggtc gatcctgact atcagcgtca gggattggga agggtgctgc tggaacatct
     5041 gatcgacgaa ctggaaaaac gcggcgtggc gacactatgg ctggaagtcc gtgcttcaaa
     5101 cgctgccgcc attgccctgt acgaaagttt aggctttaac gaggcgacga ttcgccgcaa
     5161 ttactacccc accacggacg gtcgcgaaga cgccatcatc atggcgttgc caatcagtat
     5221 gtaagacaag gtggaataat gaagtgggac tggattttct ttgatgccga tgaaacgctg
     5281 tttacctttg actcattcac cggcctgcag cggatgtttc ttgattacag cgtcaccttt
     5341 accgctgaag attttcagga ctatcaggcc gttaacaagc cactgtgggt ggattatcaa
     5401 aacggcgcga tcacttcatt acagcttcag cacgggcggt ttgagagctg ggccgaacgg
     5461 ctgaaagttg aagcaggctt gcttaacgat gcctttatta atgcgatggc ggaaatctgt
     5521 acgccgttgc cgggcgcggt ttctctgctt aacgccattc gtggcaacgc taaaatcggc
     5581 atcatcacca acggttttag cgccttgcag caagtgcgtc tggaacggac gggcctgcgt
     5641 gattacttcg atttgctggt gatttccgaa gaagttggcg ttgctaaacc gaataagaaa
     5701 attttcgatt atgcgctgga acaggcgggc aatcctgacc gttcacgcgt gctgatggtt
     5761 ggcgacactg ccgagtccga tattctcggt ggcatcaacg ccgggcttgc gacctgctgg
     5821 ctgaatgcac accatcgcga gcaaccagaa ggcatcgcgc ccacctggac cgtttcttcg
     5881 ttgcatgaac tggagcagct cctgtgtaaa cactgattgc ctcccccccg ttgatgggta
     5941 aaatagccgc aatttttcgt tttcaacaag cgcggcgcga tgccgcttac tcaagaagaa
     6001 agaattatga cgttgtctcc ttatttgcaa gaggtggcga agcgccgcac ttttgccatt
     6061 atttctcacc cggacgccgg taagactacc atcaccgaga aggtgctgct gttcggacag
     6121 gccattcaga ccgccggtac agtaaaaggc cgtggttcca accagcacgc taagtcggac
     6181 tggatggaga tggaaaagca gcgtgggatc tccattacta cgtctgtgat gcagtttccg
     6241 tatcacgatt gcctggttaa cctgctcgac accccggggc acgaagactt ctcggaagat
     6301 acctatcgta ccctgacggc ggtggactgc tgcctgatgg ttatcgacgc cgcaaaaggt
     6361 gttgaagatc gtacccgcaa gctgatggaa gttacccgtc tgcgcgacac gccgatcctc
     6421 acctttatga acaaacttga ccgtgatatc cgcgacccga tggaattgct cgatgaagtt
     6481 gagaacgagc tgaaaattgg ctgcgcaccg atcacctggc caattggctg cggcaagctg
     6541 tttaaaggcg tttaccacct ttataaagat gaaacctatc tctatcagag cggtaaaggg
     6601 cacaccattc aggaagtccg cattgttaaa gggctgaata acccggatct cgatgctgcg
     6661 gttggtgaag atctggcaca gcagctgcgt gacgaactgg aactggttaa aggcgcgtct
     6721 aacgagttcg acaaagagct gttccttgcg ggcgaaatca ctccggtatt cttcggtact
     6781 gcgctgggta acttcggcgt tgatcatatg ctggatggcc tggtggagtg ggcgcctgcg
     6841 ccgatgccgc gtcagaccga tactcgtacc gtagaagcga gcgaagataa atttaccggc
     6901 ttcgtattta aaattcaggc caacatggac ccgaaacacc gcgaccgcgt ggcgtttatg
     6961 cgtgtggtgt ccggtaaata tgaaaaaggc atgaaactgc gccaggtgcg tactgcgaaa
     7021 gatgtggtga tctccgacgc gctgaccttt atggcgggtg accgttcgca cgttgaagaa
     7081 gcgtatccgg gcgatatcct cggcctgcac aaccacggca ccattcagat cggcgacacc
     7141 tttacccagg gtgagatgat gaagttcacc ggtattccga acttcgcgcc agaactgttc
     7201 cgtcgtatcc gcctgaaaga tccgctgaag caaaaacagc tgctcaaagg gctggtacag
     7261 ctttccgaag agggcgcggt gcaggtgttc cgtccgatct ccaacaacga cctgatcgtt
     7321 ggtgcagttg gtgtgctgca gtttgatgtg gtggtagcgc gcctgaagag cgaatacaac
     7381 gttgaagcag tgtatgagtc agtcaacgtt gccactgccc gctgggtaga atgtgcggac
     7441 gcgaagaaat tcgaagagtt caagcgtaag aacgaaagcc aactggcgct tgatggcggc
     7501 gataacctcg cttacatcgc taccagcatg gttaacctgc gcctggcgca ggaacgttat
     7561 ccggacgttc agttccacca gacccgcgag cattaattct tgtctccagg gcgcggtagc
     7621 cgctgcgccc tgtcaatttc ccttccttat tagccgctta cggaatgttc ttaaaacatt
     7681 cacttttgct tatgttttcg ctgatatccc gagcggtttc aaaattgtga tctatattta
     7741 acaaagtgat gacatttctg acggcgttaa ataccgttca atgcgtagat atcagtatct
     7801 aaagccgtcg attgtcattc taccgatatt aataactgat tcagaggctg taatggtcgt
     7861 tattcatcac tcatcgcttt tgtgatggcg accattgact tctgtagagg gtgaagtctc
     7921 tccctattca gcaatgcaac ctcgtgttgc caggctcaaa ttacgagcaa acatacagga
     7981 ataaatcgat gactatgaca agactgaaga tttcgaaaac tctgctggct gtaatgttga
     8041 cctctgccgt cgcgaccggc tctgcctacg cggaaaacaa cgcgcagact accaatgaaa
     8101 gcgcagggca aaaagtcgat agctctatga ataaagtcgg taatttcatg gatgacagcg
     8161 ccatcaccgc gaaagtgaag gcggcgctgg tggatcatga caacatcaag agcaccgata
     8221 tctctgtcaa aaccgatcaa aaagtcgtga ccctgagcgg tttcgttgaa agtcaggccc
     8281 aggccgaaga ggcagtgaaa gtggcgaaag gcgttgaagg cgtgacctct gtcagcgaca
     8341 aactgcacgt tcgcgacgct aaagaaggct ctgtgaaggg ctacgcgggt gacaccgcca
     8401 ccaccagtga aatcaaagcc aaactgctgg cggacgatat cgtcccttca cgtaaagtga
     8461 aagttgaaac caccgatggc gtggttcagc tctccggtac cgtagattct caggcacaaa
     8521 gtgaccgtgc tgaaagtatc gccaaagcgg tagatggtgt gaaaagcgtt aaaaatgatc
     8581 tgaaaactaa gtaattcgtc gtaattcgtc ctcccgaact ttgtcgggag gcgcgatgtg
     8641 caccacacta aaaatatcgc attgagtgat tttcacgctc atatcaagcg gtgacattaa
     8701 ctatggtaaa ggagacgctt atgtttcgtt ggggcatcat atttctggtt atcgcgttaa
     8761 tcgccgccgc acttgggttt ggtggtctgg ccggtaccgc tgcaggcgca gctaaaattg
     8821 tctttgtcgt cgggattatt ctgttcctgg tgagtttgtt catgggccga aaacgaccct
     8881 agatttcaga ccattaagaa aattttatcc aaagccagtc cagcggactg gcttttgcgg
     8941 ttttagcgaa taataaattg cgttactttg tcattctttc acaacaaaaa caggaaggca
     9001 gaggtggggc agcgaatacc tgtaacgctt ggtaatattg cgccgttgtc gctaaggccg
     9061 ttccagcctg gacgaatagc tctggtgtgc gaaggcggcg gacagcgtgg aattttcacg
     9121 gctggcgtgc tggatgagtt tatgcgcgcg cagtttaatc ctttcgatct ttatctcggc
     9181 acatctgccg gggcgcagaa cctctcggcg tttatctgca atcagcccgg ttacgcgcgc
     9241 aaagtcatca tgcgctatac cacaaaacgc gaatttttcg atccattgcg ctttgtccgt
     9301 ggaggaaatc ttatcgatct cgactggctg gtggaggcca ccgcaagcca gatgccgttg
     9361 caaatggata ccgccgcgcg gttgtttgac agcggcaaat cgttttatat gtgcgcctgt
     9421 cgtcaggatg actacgcgcc gaattacttt ttaccaacca aacaaaactg gctggatgtg
     9481 attcgcgcct ccagtgcgat acctggcttt tatcgtagcg gagtgtcgct ggaaggcatt
     9541 aactacctgg atggcgggat cagtgatgcg attccggtta aagaggcggc aaggcagggc
     9601 gctaaaacgt tggtcgtcat ccgcactgtg ccgtcacaaa tgtactacac gccgcagtgg
     9661 ttcaaacgca tggaacgctg gctgggtgac agtagcctgc agccgctggt caatctggtg
     9721 cagcatcatg aaaccagcta tcgtgacatt cagcaattta ttgagaaacc accgggcaag
     9781 ctgcggatat tcgaaattta tccgccgaag ccattacata gtatcgcgct tggcagtcgg
     9841 attccggcgc tgcgtgaaga ctataaactt gggcgtttat gcggtcgtta tttcctcgcc
     9901 acggttggta agctattaac tgaaaaagcg ccgcttaccc gccatctggt tccagtggtg
     9961 acgccggaat cgattgtcat tccgcctgcg ccagtcgcca acgatacact ggttgccgaa
    10021 gtgagcgatg ctccgcaggc gaacgacccg acatttaaca atgaggatct ggcttgattt
    10081 gccgttttat cgacacccac tgccattttg atttcccgcc gtttagtggc gatgaagagg
    10141 ccagcctgca acgcgcggca caagcgggtg taggcaagat cattgttccg gcgacagagg
    10201 cggaaaattt tgcccgtgtg ttggcattag cggaaaatta tcaaccgctg tatgccgcat
    10261 tgggcttgca tcctggtatg ttggaaaaac atagctatgt gtctcttgag cagctacagc
    10321 aggcgctgga aaggcgtccg gcgaaggtgg tggcagtggg ggagatcggt ctggatctct
    10381 tcggcgacga ttcgcaattt gagaggcagc agtggttact cgacgaacaa ctgaaactgg
    10441 cgaaacgcta cgatctgccg gtgatcctgc attcacggcg cacacacgac aaactggcga
    10501 tgcatcttaa acgccacgat ttatcgcgca ctggcgtggt tcacggtttt tccggcagcc
    10561 tgcaacaggc agagcggttt gtacagctgg gctacaaaat cggcgtaggc ggtactatca
    10621 cctatccacg cgccagtaaa acccgcgatg tcatcgcaaa attaccgctg gcatcgttgt
    10681 tgctggaaac cgacgcgccg gatatgccgc tcaacggctt tcaggggcag cctaaccgcc
    10741 cggaacaggc tgtgcgggtg tttgacgtgc tttgcgagtt gcgcccggaa ccagaagatg
    10801 agattgccga agtgttgctt aataatacgt atgcggtgtt tagcgttagt gggtagtttg
    10861 cccttcgtag gcagcatccg gcagtcggcg cataatgcct gatgcgacgc ttgacgcgtc
    10921 ttatcatgcc tacaagtctg tgccgaaccg taggccggat aaggcgttca cgccgcatcc
    10981 ggcagttggc gcacaatgcc tgatgcgaca ctgacgcgtc ttatcatgcc tacaagtctg
    11041 tgccgaacc
//
LOCUS       DAPGOF010000165        11010 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.166, whole genome shotgun sequence.
ACCESSION   DAPGOF010000165 DAPGOF010000000
VERSION     DAPGOF010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11010
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.166"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(98..1927)
                     /gene="glmS"
                     /locus_tag="RHV13_003808"
     CDS             complement(98..1927)
                     /gene="glmS"
                     /locus_tag="RHV13_003808"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDV1243286.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2089..3459)
                     /gene="glmU"
                     /locus_tag="RHV13_003809"
     CDS             complement(2089..3459)
                     /gene="glmU"
                     /locus_tag="RHV13_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDV1243287.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3812..4231)
                     /gene="atpC"
                     /locus_tag="RHV13_003810"
     CDS             complement(3812..4231)
                     /gene="atpC"
                     /locus_tag="RHV13_003810"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDV1243288.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4252..5634)
                     /gene="atpD"
                     /locus_tag="RHV13_003811"
     CDS             complement(4252..5634)
                     /gene="atpD"
                     /locus_tag="RHV13_003811"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDV1243289.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5661..6524)
                     /gene="atpG"
                     /locus_tag="RHV13_003812"
     CDS             complement(5661..6524)
                     /gene="atpG"
                     /locus_tag="RHV13_003812"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDV1243290.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6575..8116)
                     /gene="atpA"
                     /locus_tag="RHV13_003813"
     CDS             complement(6575..8116)
                     /gene="atpA"
                     /locus_tag="RHV13_003813"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDV1243291.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8129..8662)
                     /gene="atpH"
                     /locus_tag="RHV13_003814"
     CDS             complement(8129..8662)
                     /gene="atpH"
                     /locus_tag="RHV13_003814"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDV1243292.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8677..9147)
                     /gene="atpF"
                     /locus_tag="RHV13_003815"
     CDS             complement(8677..9147)
                     /gene="atpF"
                     /locus_tag="RHV13_003815"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDV1243293.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9209..9448)
                     /gene="atpE"
                     /locus_tag="RHV13_003816"
     CDS             complement(9209..9448)
                     /gene="atpE"
                     /locus_tag="RHV13_003816"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HDV1243294.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9495..10310)
                     /gene="atpB"
                     /locus_tag="RHV13_003817"
     CDS             complement(9495..10310)
                     /gene="atpB"
                     /locus_tag="RHV13_003817"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDV1243295.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10319..10699)
                     /gene="atpI"
                     /locus_tag="RHV13_003818"
     CDS             complement(10319..10699)
                     /gene="atpI"
                     /locus_tag="RHV13_003818"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HDV1243296.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         2624 a         3002 c         2743 g         2641 t
ORIGIN      
        1 taaattcata ttgctgagtt tatagataaa acaattattg acgttattga tgaaaagggc
       61 caccagatgg cagcccttat ttgatgaatg aagagtatta ctcaaccgtt accgattttg
      121 ccaggttacg cggctggtca acgtcggtgc ctttgatcag cgcgacgtga taagccagta
      181 actgcagcgg aacggtgtag aagatcggtg caatcacctc ttccacatgc ggcatctcga
      241 tgatgtgcat gttatcgcta cttacaaaac ccgcatcctg atcggcgaag acatacaact
      301 gaccgccacg cgcgcgaact tcttcaatgt tggatttcag tttttccagc aattcgttgt
      361 tcggtgcaac gacgataacc ggcatatccg cgtcaatcag cgccagcggg ccgtgtttta
      421 gctcgcccgc agcgtaggct tcagcgtgaa tgtaagagat ctctttcagc ttcaatgcgc
      481 cttccagcgc gattgggtac tgatcgccac ggcccaggaa cagcgcgtga tgtttgtcag
      541 agaaatcttc tgccagagct tcaatgcgtt tgtcctgaga cagcatctgc tcaatacggc
      601 tcggcagcgc ctgcagacca tgcacaatgt catgttcaat ggaggcatcc agacctttca
      661 ggcgagacag cttcgccacc agcatcaaca gcacagttaa ctgagtggtg aatgctttag
      721 tggatgccac gccgatttcc gtacccgcgt tggtcatcag cgccagatcg gattcgcgca
      781 ccagagaaga acccggaacg ttacagattg ccagcgaacc aaggtaaccc agctctttcg
      841 acagacgcag gcccgccagg gtatccgcgg tttcgccaga ctgtgacaag gtgatcatca
      901 ggctgttacg acgcacggca gatttacgat agcggaattc agaggcgatt tcgacgtcgc
      961 acggaatacc tgccagcgat tcaaaccagt aacgggaaac cataccggag ttataagaag
     1021 taccacaggc gaggatctga atatgctcaa ccttcgacag cagttcgtcg gcgttcggtc
     1081 ccagctcgct taaatcaacc tgaccgtggc tgatgcgtcc ggtaagggta tttttgatcg
     1141 cgttcggctg ttcgtagatc tctttctgca tgtagtggcg gtaaatacct ttatcgcccg
     1201 cgtcatattg cagattggat tcgatatcct gacgttttac ttccgcgcca gttttatcga
     1261 agatgtttac cgaacggcga gtgatttccg caatatcgcc ctcttcaagg aagataaagc
     1321 gacgggtcac cggcagcagc gccagctggt cagaagcgat aaagttttca cccataccca
     1381 gaccaatcac cagcggacta ccagaacgtg ctgccagcag ggtatccggg tgacgggagt
     1441 ccatgatcac tgtaccgtac gcaccacgca gttgcgggat agcacgcaga acagcctcac
     1501 gcagagtacc gccctgtttc agctcccagt tcaccaggtg tgcaatcact tcggtgtcgg
     1561 tttcagaaac gaaggtatag ccacgcgctt ttaactcttc acgcagcggt tcatggtttt
     1621 cgatgatgcc gttatgcacc accacaatgt gttcagaaac atgcggatgc gcgttagctt
     1681 ctgaaggttc accgtgggtc gcccagcgag tgtgagcaat accggtgccg ccatgcagag
     1741 gatgttcttc cgctgcctga gccagcatct ggactttacc gaggcgacgc aggcgggtca
     1801 tatgaccttc cgcatcaaca acggccagac cggcagagtc atatccgcgg tattccagac
     1861 gacgtaaacc ttcaagaagg atttctgcta catcacgttg cgcgatcgcg ccaacaattc
     1921 cacacatagt ttttgattcc gatttatatc gttgtcggtc aacctgtatg cccgtttttt
     1981 cgggcgcccc gagccttgta gagagtgggg ttatttttat agttactgct tgtgggaggg
     2041 agattatgtt atctcctcat cccagatgac cggattaccc ggccagaatt actttttctt
     2101 taccggacga cgccagcctt ctttctgagt ctgcggcaca cggctgatag ctaatgcatt
     2161 ttcgccgaca ttacgcgtca cagttgtacc cgcagcaatg gtcgcgcctt tgcctactgt
     2221 taccggggcc accagctgag tgtcggaacc gacaaacaca tcgtcgccga taatggtctt
     2281 aaatttattc gcaccgtcgt agttgcaggt aatggttccc gcgccgatgt taacgttatc
     2341 gccaatttcc gcatcgccca ggtaagtcag atgaccagct ttcgagcctt tacccagacg
     2401 cgcttttttc atctcaacaa agttaccgac gtgtgcacct tccagcaact cagcaccagg
     2461 acgcagacgg gcaaacgggc caatagtaca ggccgctgcc agattcgcgt cttccacaac
     2521 ggtatacgga ctgatttcgc aatcatcgcc aatcacgctg tttttaatca cgcaaccggt
     2581 gccgattttc acgcgatgac cgagagtcac gttgccctcg atgataacgt tagtatcaat
     2641 ttcaacatcg cgcccgtgag taagcgtacc gcgcagatca aaacgcgccg gatcgcgcag
     2701 cataacgcct gctaacagca gtttttcagc ctgttcggac tggtaaacgc gctccagacg
     2761 ggagagttgc aggcggttat tcacgccttc tacttcgctt aaacgttgcg gatgaacggc
     2821 gacgatttca cgcccttcct gatacgccag cgcaataatg tcggtgatgt agtattcgcc
     2881 ctgagcatta ttgttggtca gcttcgccag ccagcgtttc atatctgcgc cgttagcgat
     2941 cagaatgccg gtgttgatct cctgaatctg acgctgctcg tcggtggcat ctttgtgctc
     3001 aacaatgccg gtaactttgc cattttcacg ggtgatacgt ccataaccgg tcggatcatc
     3061 cagtttcacc gtcagcagac caatgccacc ctgcggttta gcatcacgca ggcgctggag
     3121 tgtttcgaca gagatcagcg gcacgtcgcc gtagagcatt aaaatgtctt catcatcggc
     3181 aaagaaaggt gcggcctgct gcattgcatg acccgtaccc agctgctctg cctgaagcac
     3241 ccagttaagg ttgtcgtctt tcagcgcctg tttaagcaga tcgccgccgt gaccgtacac
     3301 caggtgaacg tgcgctgcgc ctaattcatt cgcagcatca atgacatgct gaaccatcgc
     3361 tttcccggca agggtatgca gcactttcgg aagatcggaa tacatgcgcg tgcctttgcc
     3421 tgcggcaagg atcactacgc tcatagcatt attcaacata cgcgtcctga ctgtaatttg
     3481 agaacgaatt taaaccgctt caccttgaaa aaactacatt tttttcatcg tgaaatggac
     3541 agaggataaa ttgttcaata acggattatc cccgctgacg aaagtgccat tttcgaccat
     3601 cgtcacgcct tttgtctgca gaaaattaag acagtacctt catttaagcg tcaggtggat
     3661 gtttttgctc ttatttcgat caatgaataa acgggaaaac agggggtttt atatcagcag
     3721 gatctatgtg aacgctattc aggacgggtc acacgcgcaa aaaaaaagcc agcctgtttc
     3781 cagactggct tttgtgcttt tcaagccggt gttacatcgc ttttttggtc aactcgataa
     3841 cgcgcagctg cgcgatcgct ttggccagtt ccgcagacgc ctgagcgtaa tctacgtcgc
     3901 cgtgagagct gctaatgtgc tcttcagcct tacgtttcgc ttccatggct cgcgcttcgt
     3961 cgagatcctg gccgcgaatt gcggtgtcgg ccagaacggt cacgttgcca ggctgcactt
     4021 caagaatgcc gccagacaga tagataaact cttcgtgacc gtgctgtttc acgatgcgaa
     4081 tcataccagg cttaatggcg gtgagcagcg gtgcgtggcc agggtagatc cccagttcac
     4141 cttcgctacc cgttacctgg attttctcga ccagaccaga gaacatttgt tgctctgcgc
     4201 tgacgacgtc caggtggtaa gtcattgcca tatcaccctc cgattaaggc gttaaagttt
     4261 tttggctttt tccacagctt cttcgatgga accgaccatg tagaacgcct gctccggcag
     4321 gtgatcgtat tcgccttcca tgatgccttt aaagccacgg atggtgtctt tcagggagac
     4381 gtatttaccc ggagaaccgg tgaatacttc tgccacgaag aacggctggg acaggaagcg
     4441 ctggatctta cgagcacgcg ctaccaccag tttgtcttct tcagacagtt catccatacc
     4501 caggatggcg atgatgtctt tcagttcctg ataacgttgc aggatggact gaacgccacg
     4561 cgcggtgtcg tagtgttcct gaccaaccac cagcgggtcc agctgacggc tggtggagtc
     4621 cagtgggtca acggccgggt agatacccag agacgcgatc tgacggctca gtaccacggt
     4681 tgcgtcaagg tgcgcaaagg tggttgccgg agacgggtca gtcaagtcat ccgcaggtac
     4741 gtataccgcc tgtacggagg tgatagaacc agttttggtg gaggtgatac gttcctgcag
     4801 aacgcccatc tcttccgcca gggtcggctg ataacctacc gctgaaggca tacggcccag
     4861 cagtgcggat acttccgtac cggccagggt gtaacgatag atgttgtcaa cgaacagcag
     4921 aacgtcacga ccttcgtcac ggaatttctc agccatggtc agaccggtca gagcaacgcg
     4981 cagacggttt cccggcggct cgttcatctg gccatacacc agggatactt tgtcgataac
     5041 gttggagtcg gtcatttcgt ggtagaagtc gttaccctca cgagtacgtt cacctacgcc
     5101 cgcaaacaca gagtaaccgg agtgctcgat cgcgatgtta cgaatgagct ccatcatgtt
     5161 tacggtttta cctacacccg caccaccgaa cagaccaact ttaccgccct tagcgaacgg
     5221 acacatcagg tcgataactt tgataccggt ttccagcagt tcctgagagt ttgacagctc
     5281 ttcgtaggaa ggtgctgcgc ggtgaatcgc ccaacgctct tcttcaccga tctcgccttt
     5341 catgtcgacc ggttcaccca gtacgttcat gatacggccc agagtcgctt tacctaccgg
     5401 gacttcgatc gggtgttcga ggtcttttac atccagaccg cgacgcagac cgtcggagga
     5461 acccattgcg atggtacgca cgataccgcc gccgagctgc tgctgaactt ccagcaccag
     5521 acgctcatta ccattttgca cctcaagagc atcgtacacg cgcggtacgg catcctgagg
     5581 gaattcgacg tcaactacgg cgccgattac ctggacaatc tttccagtag ccatcttaaa
     5641 tcctctacga aataacctgt ttaaaccgcg gcggcccccg agacgatctc ggtgagttcc
     5701 tgagtaatgc tggcctgacg agctttgttg tataccaact gcagctcttt aatcaggctg
     5761 ccgccattgt cggtcgcggc tttcatcgcc accatacggg cggcctgctc gctggccagg
     5821 ttttcaacca cgccctgata aacctgagat tcgacataac gacgcagcag ggtatccagc
     5881 aacgccttcg gatcgggttc gtacaggtaa tcccaggatt tatgtttcag atcatcatca
     5941 tctgatgccg gtaacggcag cagctggctg atggtcggaa cctgagacat ggtgttaata
     6001 aatttgttgc tgacaatgta aagcttgtcc agacggcctt cgtcgtaggc ctgcaacatc
     6061 acttttaccg gaccgatcag ttcggacagg gaagggttat cccccatgcc ggtgacctgg
     6121 gcaacaacat tgccgcccac ggagttgaag aacgacacgc ctttcgagcc gatcattgcg
     6181 aggtcgcatt gaacgccttt gtcggtccag gtcttcattt ccgccagcag ttttttgaac
     6241 aggttaatgt tcaaaccacc gcacaaacca cggtcggtcg acaccaccag gtagcccacg
     6301 cgtttaacgt cgcggtcttc caggtaaggg tgtttgtatt ccagattacc gtgtgcaagg
     6361 tgaccaatca ctttgcgcat ggtttctgca taaggacggc tggccgccat gcgatcctgc
     6421 gatttacgca ttttggaagc ggcgaccatc tccatcgctt tagtgatctt ttgcgtgttc
     6481 tggacgcttg cgatcttact acgtatctct tttgcgccgg ccatgagctt ctcctcaatg
     6541 ccttgcggcc tgccctaagg caagccgcca gacgttacca ggattgggtt gctttgaagg
     6601 aatcgaggat gcctttcagc ttgccttcga tttcgtcgtt gtagccaccg gtctggttga
     6661 tctcttgcat caacggagcg tgatcacggt cgacgtaagc cagcagagcg gcttcgaagc
     6721 tgccaatttt cgacagttca acatccgcca ggtaaccacg ttctgctgcg aacagaacca
     6781 gagactgctg cgcaacggac atcggcgcat actgtttctg tttcagcagt tcggtcactt
     6841 tctgaccgtg gtcaagctgc ttacgtgttg catcgtcaag gtcggatgca aactgagaga
     6901 acgctgccag ttcacgatac tgtgccagag cggtacggat accaccggac agttttttca
     6961 tgatcttggt ctgtgctgca ccaccaacac gggatacgga aatacccggg ttaaccgcag
     7021 gacgaatacc ggcgttgaac aggttggttt ccaggaagat ctgaccatcg gtaatggaga
     7081 ttacgttggt cggaacgaac gcagaaacgt cacccgcctg agtttcgata atcggcagtg
     7141 cggtcagaga accggttttc cctttcactt cacctttggt gaaggcttca acgtattcgg
     7201 cgttaacacg tgcagcacgc tccagcagac gagagtggag gtagaaaacg tcgcccggga
     7261 atgcttcacg tcctggcgga cgacggagca gcagggagat ctgacggtaa gcaacagcct
     7321 gtttagacag gtcatcgtaa atgatcagcg catcttcacc gcggtcacgg aagtattcgc
     7381 ccattgcgca accggcatac ggtgccaggt attgcagtgc agcggattca gacgcggttg
     7441 ctaccacaac gatggtgtta gccagtgcgc cgtgctcttc cagtttacgt accacgttag
     7501 aaatggtgga cgctttctgg ccgatagcga catagataca tttgataccg gaatcgcgct
     7561 ggttgatgat ggcatcgata gccagtgcgg ttttaccggt ctgacggtca ccgatgatca
     7621 attcacgctg accacgaccg attgggatca tggagtcaac ggctttataa ccggtctgta
     7681 ccggctgatc tacggactga cgttcgataa cgcccggagc gattgcttct acagcagaga
     7741 agccgtcgtg atccagcgga cctttaccgt cgattggtgc acccagagtg ttaaccacac
     7801 ggcccagcag gccacggcca accggaactt ccaggatacg gccagtacac ttaactttca
     7861 tgccttcggc aaggtcagcg tacggaccca taacaaccgc acctacagag tcgcgctcga
     7921 ggttcagtgc gatagcgtaa cggttacccg gcagggagat catttcaccc tgcatacaat
     7981 cggccaggcc gtgaatgcgg ataacaccgt cacttacaga aacaatagta ccttcgttgt
     8041 gagcttcact cacaacattg aactgagcaa tgcgctgctt gatcagttcg ctgatttcgg
     8101 tggaattcag ttgcatgctc cagtcccctt aagactgcaa gacgtctgca aggcgctcaa
     8161 gacgaccgcg tacgctgcca tcaatgacca tatcacccgc tcggatgata acgcctgcca
     8221 ttacagactt atcgattttg caattcagct taactttgcg tgacagacgt ttttccatcg
     8281 cagcagaaat tttcgcgagc tgttgttcac tcaatgcagc agcggaaatg acgtctacct
     8341 cagcggtagc ctcactaacg gcacgcaggt gaataaactg ctccagaaca tccgggagcg
     8401 cgttaagacg accattctca gccattaccc gaatcaggtt ctgaccgttt tcgtccagtt
     8461 gctcaccaca aactgcgata aacgactcgg cgagcgtttc tggcgcaagc gcgccagaga
     8521 gaagctctgc catttgttcg tttttggtta cctcggcggc aaacgccagc atgtcctgcc
     8581 agcgttctac actttggtgt tcgacggcaa agtcaaaagc tgctttggcg taggggcgag
     8641 ctaccgtaat aaattcagac atcagcccct ccctccttac agttcagcga caagtttatc
     8701 cacgatgtcg ctgttagcag cttcatccac ggaacgttcg atgatcttct cggcgccagc
     8761 aacagccagg atagcaactt gcttacgcag ctcttcacgg gcacgtttac gctcggcttc
     8821 aatttccgcc tgcgcctggg ccacgatttt agtacgttcc tgttctgcct cagctttcgc
     8881 ttcgtccaga atctgcgagc ggcgtttgtt cgcctgctcg atgattacct gggcttccgc
     8941 tttcgctttt ttcagctggt cggtcgcgct ggcctttgca aggtcaaggt ccttatgtgc
     9001 tcgttctgcg gaagcaaggc cgtcagcaat ttctttttga cgtttttcga tggctgccat
     9061 taatggcggc catacgtact tcatgcagaa cagaacgaac aggacaaacg cgatggcctg
     9121 gccgaggatt gttgcgttaa gattcacagc acaatgcctc tatttagtta acgttctgat
     9181 attgctcttt aaataaaagc aacgcttact acgcgacagc gaacatcacg tacagaccca
     9241 gacctacagc gatcatcggg atagcatcca ccagacccat aacgataaag aactgagtac
     9301 gcagcagagg aatcagatca ggttgacgcg ctgcgccttc caggaattta cccccgagga
     9361 tgccgatacc gatcgcagca ccgattgccg ccagacccat catcacagcg gcagccatgt
     9421 acagcagatc catattcagg ttttccatga cagtctccag tttgtttcag ttaaaacgta
     9481 gtagtgttgg taaattaatg ttcttcagac gccatcgaca gatagacgat cgtcagaacc
     9541 atgaagatga aggcttgcag cgtaatgatc aggatgtgga aaatggccca cggcacattc
     9601 aggatccact gtgaccacca cggcaacaga ccagcaatca gaatgaaaat cagctcaccg
     9661 gcatacatgt taccgaacag tcgcaaaccg agtgaaactg gtttggacag caggcttacc
     9721 ccttcaagga ttaagttgac aggaatgaac gcccagtgat tgaacggctg cagcgtcaac
     9781 tctttcgtga agccgccgat gcctttcatt ttgatgctgt agaacagaat caggataaat
     9841 acgcccagtg ccatagacag cgttacgttc acgtccgcag acggaaccac acgcagtgca
     9901 ggcagaccca gtacatgttc agcaatgtac ggcagcaggt cgataggcag taaatccatc
     9961 aggttcatca ggaataccca gacgaagatc gtcagggcca gcggagcaat cagcttgctt
    10021 ttgccatggt acatgtcttt cacgctacca ttaacaaagc cgatcaccag ctcaatcgcg
    10081 gtctgaaact tacctggcac accgctggtc gcctttttgg ctacgctacg gaataaaacc
    10141 aggaacaaca gacccagcac caccgagaag aacatggagt caatattgat tgtccagaag
    10201 gtggctgggg ggttttgtgg atccaccagc gagaatgtac gcaggtccag ctgaaggtta
    10261 ttcaggtggt gtcctatgta atcctgcggc gtcatatttt ctgaagccat gatgcctttt
    10321 accctttgtt gttaattaca gccggtgcca gtatctgaac caccagcacc aaaacccacg
    10381 taacgatcag cggcaagaat accgccttta aaaccgccaa cgccaccacc agtaacacca
    10441 acatcgccag aactttgaaa gcttcgccaa atgcgaatgt ccaggccacc cggcctttcg
    10501 ctggtgtatg cgcctggtga cgccaggcaa atatcataaa caaaacgtta ggcagaaaga
    10561 ctgccaggcc cccgcttatt gcagagacgc cccagaaggg gtctttgagg ctgaacagca
    10621 atccacttgc tatcaccacc agtaactgaa cgagcagaag cttccgagca acgtttcgac
    10681 tcacgagcga cacagacatc acgtttttca ctcctgctcc cttcgaggta tgccgcgtgt
    10741 cgtataaaac tttctttaag gcttagagtc aagcatcaaa aagcggtcaa attatacggt
    10801 gcgcccccgt gatttcaaac aataagtagc caaaaggtga ataaatgttt aaatattttt
    10861 ccagtgcata caattgcgac ttttctgcta accctgttcg atcatgaaaa actgtaaata
    10921 acgcgtaaac actggtgata aagcgtgctt cagatcacat attgcgcatg ttcgcgcaca
    10981 gcatatttat ttacttggca aatgatgcct
//
LOCUS       DAPGOF010000166        10928 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.167, whole genome shotgun sequence.
ACCESSION   DAPGOF010000166 DAPGOF010000000
VERSION     DAPGOF010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10928
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.167"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            72..1214
                     /locus_tag="RHV13_003819"
     CDS             72..1214
                     /locus_tag="RHV13_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDV1243297.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGKDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(1221..1985)
                     /gene="glcC"
                     /locus_tag="RHV13_003820"
     CDS             complement(1221..1985)
                     /gene="glcC"
                     /locus_tag="RHV13_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HDV1243298.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNLS"
     gene            2236..3735
                     /gene="glcD"
                     /locus_tag="RHV13_003821"
     CDS             2236..3735
                     /gene="glcD"
                     /locus_tag="RHV13_003821"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HDV1243299.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3735..4787
                     /gene="glcE"
                     /locus_tag="RHV13_003822"
     CDS             3735..4787
                     /gene="glcE"
                     /locus_tag="RHV13_003822"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HDV1243300.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTAEDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4798..6021
                     /gene="glcF"
                     /locus_tag="RHV13_003823"
     CDS             4798..6021
                     /gene="glcF"
                     /locus_tag="RHV13_003823"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HDV1243301.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERILREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMPANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            6026..6430
                     /locus_tag="RHV13_003824"
     CDS             6026..6430
                     /locus_tag="RHV13_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein GlcG"
                     /protein_id="HDV1243302.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6452..8623
                     /gene="glcB"
                     /locus_tag="RHV13_003825"
     CDS             6452..8623
                     /gene="glcB"
                     /locus_tag="RHV13_003825"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HDV1243303.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRHYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            8978..10660
                     /gene="glcA"
                     /locus_tag="RHV13_003826"
     CDS             8978..10660
                     /gene="glcA"
                     /locus_tag="RHV13_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HDV1243304.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGIITLLQAYVFTGMLVS"
BASE COUNT         2552 a         2755 c         3054 g         2567 t
ORIGIN      
        1 gattattaag gtgccgttcg ttgctgttgc aacctgtttg tcaccgttaa ttatttgtta
       61 cccacagaac tatggaatgc gatctgctga tgattaaaat tgaaaaagta ataaataaaa
      121 acgacttaaa ggcatttatc gcttttccgt catcacttta tcccgacgat ccaaactgga
      181 tacccccttt attcattgaa cgcaacgagc atttgtctgc gaaaaatccg gggacggacc
      241 atatcatctg gcaggcgtgg gtggcaaaaa aagcggggca gatagttggg cgtattaccg
      301 cgcaaataga taccttgcac cgcgagcgtt acggcaaaga taccggtcat ttcggcatga
      361 ttgacgccat tgatgatccg caggtttttg ccgcgttgtt tggcgcagcg gaagcgtggt
      421 tgaagtccca aggtgcaagt aagatcagcg gtcctttcag cctgaatatc aatcaggaaa
      481 gcggattact gattgaaggt tttgacacac caccctgtgc gatgatgcca cacggcaaac
      541 cgtggtatgc cgcgcatatt gaacaactgg gttatcacaa aggcattgat ttactggcgt
      601 ggtggatgca gcgaaccgat ctcactttct ctccggcgct aaaaaaactg atggatcagg
      661 tgcgcaaaaa ggtgaccatt cgctgcatca atcgccagcg gtttgccgaa gagatgcaga
      721 tcctgcgtga gattttcaac tctggctggc aacacaactg gggatttgtg ccgtttaccg
      781 aacatgaatt cgcgaccatg ggcgatcaac ttaaatatct ggtgccggac gatatgatct
      841 acatcgctga gattgattct gcaccctgcg cgtttattgt cggcttgccg aacatcaacg
      901 aggcgattgc cgatctgaac ggatcgctct ttcccttcgg ctgggcaaaa ttgctgtggc
      961 gtttgaaagt cagcggtgtg cgaactgcgc gagtaccgct gatgggcgtg cgcgacgagt
     1021 atcagttcag ccgcatcggc ccggtgattg cgctgttatt gattgaagcc ttacgcgatc
     1081 cgtttgcccg ccggaagatc gatgcgctgg agatgtcatg gatcctcgaa accaatacag
     1141 gcatgaataa catgctggaa cgcattgggg cggagccgta caagcgttac cgattgtatg
     1201 aaaagcagat ttaataccag ctaactcagg ttcatctcca gcggcaccgc cgagcgaatc
     1261 aaatggtggc cttccagctc gatatcgtgg agattctttt tcacggtccg cacatgatcg
     1321 cgtgctgcgc gctgggcgac gtgcggcagc cgctgcaaca ccgcgttgta gatccgcgca
     1381 tgctggcgat cgatctgctt tttttgctgt ggtcgatggt agagattatt tacgctggca
     1441 aacactgaat taaacatcag gtcggtcaat gattgcagcg taaacaccag cacctgattg
     1501 tgagaagcct gacaaatggc gagatggaaa gcgtgatcca actgcgcatg ttcgatcagc
     1561 gaaatctctt tgttgttctc actggcggcg agcatttttt cataacagcg ggttatcaca
     1621 acaaaatcag cctgcgttcc cagcgttgcc gccagccttg ccgattcgcc ctccagcaat
     1681 gcgcgaacgt cgagcagatc gtacagcgtt cgcggctgcg tgctgaacag atgaattagc
     1741 gggctggtgt cctgcacccg attaagccgt gcgacacgag aatcgcgacc ctgcgccgtt
     1801 tcaataatcc cgcgcccgcg cagcacggtc agcccttcac gcagtgcgga gcgtgagaag
     1861 ccgagctttt cacacagtcg acgttccgag ggaagcggct gaccgacctt cagtacgccg
     1921 tcgataatta accgttcgat actctctgca accacttcgc aaatagggcg acgttcatct
     1981 ttcattcccg gacctcgtgc acaggtagga ccaatttttc tgtacttaac tcgcaaaaca
     2041 cgcacatcac gtaagtgatt atgttacatc aatttaacat tgagttaacc aaggcaaggt
     2101 cacagagctg gaaaaaaaat ggtctgaccg gtagctaaag agatagacga aaacgaaaag
     2161 cccgcttaat aactgttcac agaagcagcg cgcaaaaatc agctgccaca caacacaact
     2221 aagcgaagcc tactcatgag catcttgtac gaagagcgtc ttgatggcgc tttacccgat
     2281 gtcgaccgca catcggtact gatggcactg cgtgagcatg tccctggact ggagatcctg
     2341 cataccgatg aggagatcat tccttacgag tgtgacgggt tgagcgcgta tcgcacgcgt
     2401 ccattactgg ttgttctgcc taagcaaatg gaacaggtga cagcgattct ggctgtctgc
     2461 catcgcctgc gtgtaccggt ggtgacccgt ggtgcaggca ccgggctttc tggtggcgcg
     2521 ctgccgctgg aaaaaggtgt gttgttggtg atggcgcgct ttaaagagat cctcgacatt
     2581 aaccccgttg gtcgccgcgc gcgcgtgcag ccgggcgtgc gtaacttggc gatctcccag
     2641 gccgttgcac cgcataatct ctactacgca ccggaccctt cctcacaaat cgcctgttcc
     2701 attggcggca acgtggcaga aaatgccggc ggcgtccact gcctgaaata tggtctgacc
     2761 gtacataacc tgctgaaaat tgaagtgcaa acgctggacg gcgaggcact gacgctggga
     2821 tcggatgcgc tggattcgcc tggttttgac ctgctggcgt tgttcaccgg atcggaaggt
     2881 atgctcggcg tgaccaccga agtgacggta aaactgctgc cgaagccgcc cgtggcgcgg
     2941 gttctgttag caagctttga ctcggtagaa aaagccggac ttgcggttgg tgacatcatc
     3001 gccaatggca ttatccccgg cgggctggag atgatggata acctgtcgat ccgcgcggcg
     3061 gaagatttta ttcatgccgg ttatcccgtc gacgccgaag cgattttgtt atgcgagctg
     3121 gacggcgtgg agtctgacgt acaggaagac tgcgagcggg ttaacgacat cttgttgaaa
     3181 gcgggcgcga ctgacgtccg tctggcacag gacgaagcag agcgcgtacg tttctgggcc
     3241 ggtcgcaaaa atgcgttccc ggcggtagga cgtatctccc cggattacta ctgcatggat
     3301 ggcaccatcc cgcgtcgcgc cctgcctggc gtactggaag gcattgcccg tttatcgcag
     3361 caatatgatt tacgtgttgc caacgtcttt catgccggag atggcaacat gcacccgtta
     3421 atccttttcg atgccaacga acccggtgaa tttgcccgcg cggaagagtt gggcgggaag
     3481 attctcgaac tctgcgttga agttggcggc agcatcagtg gggaacatgg catcgggcga
     3541 gaaaaaatca atcaaatgtg cgcccagttc aatagcgatg aaatcacgac cttccatgcg
     3601 gtcaaggcgg cgtttgaccc cgatggtttg ctgaaccctg ggaaaaacat tcccacgcta
     3661 caccgctgtg ctgaatttgg tgccatgcat gtgcatcacg gtcatttacc ttttcctgaa
     3721 ctggagcgct tctgatgcta cgcgagtgtg attacagtca ggcgctgctg gagcaggtga
     3781 atcaggcaat tagcgataaa acgccgctgg tgattcaggg cagcaatagc aaagcctttt
     3841 taggtcgccc agtcaccggg caaacgctgg atgtgcgttg tcatcgcggc attgttaatt
     3901 acgacccgac cgagctggtg ataaccgctc gtgccggaac gccgctggtg gcgattgaag
     3961 cggcgctgga gagcgcaggg caaatgcttc cctgcgagcc gccgcattat ggtgaagaag
     4021 ccacctgggg cgggatggtc gcctgcgggc tggcggggcc gcgtcgcccg tggagcggtt
     4081 cggtccgcga ttttgtcctc ggcacgcgca tcattaccgg cactggaaaa catctgcgtt
     4141 ttggtggcga agtgatgaaa aacgttgccg gatacgatct ctcacggtta atggccggaa
     4201 gctacggttg tcttggcgtg ctcactgaaa tctcaatgaa agtgttaccg cgaccgcgcg
     4261 cctccctgag cctgcgtcgg gaaatcagcc tgcaagaagc catgagtgaa atcgccgagt
     4321 ggcaactcca gccattaccc attagtggct tatgttactt cgacaatgcg ttgtggatcc
     4381 gccttgaggg cggcgaagga tcggtaaaag cagcgcgtga actgctgggt ggcgaagagg
     4441 tggcgggtca gttctggcag caattgcgtg aacaacaact gccgttcttc tcgttaccag
     4501 gtaccttatg gcgcatttca ttacccagcg atgcgccgat gatggattta cccggcgagc
     4561 aactgatcga ctggggcggg gcgttacgct ggctgaaatc gacagccgag gacaatcaaa
     4621 tccatcgcat cgcccgcaac gctggcggtc atgcgacccg ctttagtgcc ggagatggtg
     4681 gctttgcccc gctatcggct cctttattcc gctatcacca gcagcttaaa cagcagctcg
     4741 acccttgcgg cgtgtttaac cccggtcgca tgtacgcgga actttgagga gcaggctatg
     4801 caaacccaat taactgaaga gatgcggcag aacgcgcgcg cgctggaagc cgacagcatc
     4861 ctgcgcgcct gtgttcactg cggattttgt accgcaacct gcccaaccta tcagcttctg
     4921 ggcgatgaac tggacgggcc gcgcgggcgc atctatctga ttaaacaggt gctggaaggc
     4981 aacgaagtca cgcttaaaac acaggagcat ctcgatcgct gcctcacttg ccgcaattgt
     5041 gaaaccacct gtccttctgg tgtgcgctat cacaatttgc tggatatcgg gcgtgatatt
     5101 gtcgagcaga aagtgaaacg cccactgccg gagcgaatac tgcgcgaagg attgcgccag
     5161 gtagtgccgc gtccggcggt cttccgtgcg ctgacgcagg tagggctggt gctgcgaccg
     5221 tttttaccgg aacaggtcag agcaaaactg cctgctgaaa cggtgaaagc taaaccgcgt
     5281 ccgccgctgc gccataagcg tcgggtttta atgctggaag gctgcgccca gcctacgctt
     5341 tcgcccaaca ccaacgcggc aactgcgcga gtgctggatc gtctggggat cagcgtcatg
     5401 ccagctaacg aagcaggctg ttgtggcgcg gtggactatc atcttaatgc gcaggagaaa
     5461 gggctggcac gggcgcgcaa taatattgat gcctggtggc ccgcgattga agcaggtgcc
     5521 gaggcaattt tgcaaaccgc cagcggttgt ggcgcgttcg tcaaagagta tgggcagatg
     5581 ctgaaaaacg atgcgttgta tgccgataaa gcgcgtcagg tcagtgagct ggcggtcgat
     5641 ttagtcgaac ttctgcgcga ggaaccgctg gaaaaactgg caattcgcgg cgataaaaaa
     5701 ctggccttcc actgcccgtg taccctacaa catgcgcaaa agctgaacgg cgaagtggaa
     5761 aaagtgttgc ttcgtcttgg atttacctta acggacgttc ccgacagcca tctgtgctgc
     5821 ggttcagcgg gaacatatgc gttaacgcat cccgatctgg cacgccagct gcgggataac
     5881 aaaatgaatg cgctggaaag cggcaaaccg gaaatgatcg tcaccgccaa cattggttgc
     5941 cagacgcatc tggcgagcgc cggtcgtacc tctgtgcgcc actggattga aattgtagaa
     6001 caagcccttg aaaaggaata acaaaatgaa aactaaagtc attcttagcc agcaaatggc
     6061 gagtgcaatt attgccgcag gtcaggaaga ggcgcagaaa aataactggt ctgtttccat
     6121 tgctgttgcc gatgacggcg gtcatctgct ggcgttaagt cgcatggacg attgcgcgcc
     6181 gattgcggct tacatctccc aggagaaagc gcgtaccgcc gcgctggggc gtcgtgaaac
     6241 taagggctat gaagagatgg tgaacaacgg acgtaccgcg ttcgtgactg cgccgttatt
     6301 aacgtcgctg gaaggcggcg taccggttgt tgttgatggg caaattattg gtgccgtggg
     6361 cgtttctggt ttaaccggag cacaggatgc gcaggtcgcg aaagcggcag cagcggtgtt
     6421 ggcgaaataa gcgaaaacga ggagataaac aatgagtcaa accataaccc agggccgttt
     6481 acgcattgac gccaatttta aacgttttgt ggatgaagaa gttttaccgg gcgtggaact
     6541 ggacgctgcc gcattctggc acaatgttga tgagatcgtt cacgatctgg caccagaaaa
     6601 tcgtcagttg ctggcagagc gcgatcgcat tcaggcggcg cttgatgagt ggcatcgcag
     6661 taatccgggg ccggtaaaag ataaagcggc ctataaatct ttcctgcgtg aactgggcta
     6721 cctggtgccg caaccggagc gcgtgacggt ggaaaccacg ggcatcgaca gcgaaatcac
     6781 cagccaggcg gggccacagc tggtggttcc ggcaatgaac gcccgctacg cgctgaacgc
     6841 ggcgaacgct cgctggggct cactgtacga tgcgttatac ggcagcgaca tcatcccgca
     6901 ggaaggggcg atggtcagcg gctacgatcc gcaacgcggt gagcaggtta tcgcctgggt
     6961 tcggcgtttc ctcgatgaat ctctaccgct ggaaaacggc agctatcagg atgtggtggc
     7021 gtttaaggtg gtcgataaac aattacgcat ccagttgaaa aatggtaaag aaaccacgtt
     7081 acgtaccccg gcgcagtttg tcggttaccg tggcgatgcc gctgcgccga cctgcatttt
     7141 gctgaaaaat aacggcctgc atattgaact gcaaattgat gccaacgggc ggattggcaa
     7201 agacgatccg gcgcacatca acgatgttat cgtcgaagcg gccatcagta ccattctcga
     7261 ctgcgaagat tcggtcgcgg cggttgatgc ggaagataaa atcctgctgt accgcaatct
     7321 gctgggcctg atgcagggga ctctgcaaga gaaaatggag aaaaacggtc gacaaatcgt
     7381 gcgtaaactg aatgacgatc gtcattacac cgccgccgat ggctctgaaa tttctctgca
     7441 cggacgctcg ctgctgttta tccgcaacgt gggtcatttg atgaccattc ctgtgatttg
     7501 ggacagcgaa ggcaatgaaa tcccggaagg cattcttgat ggcgtcatga ctggcgcgat
     7561 tgccctctat gatttaaaag tgcagaaaaa ctcgcgcact ggcagcgtct atattgtgaa
     7621 accgaaaatg cacggtccgc aggaagtggc gttcgccaac aaactgttta cccgcattga
     7681 gacaatgctc ggtatggcac cgaataccct gaaaatgggc attatggatg aagaacgtcg
     7741 gacctcgctg aacttgcgta gctgtatcgc tcaggcgcgc aaccgcgtgg cgttcatcaa
     7801 taccggtttc ctcgaccgta ccggcgatga aatgcattcg gtgatggaag ctggcccgat
     7861 gctgcgtaaa aatcagatga aatcgacgcc ttggatcaaa gcctacgagc gtaataacgt
     7921 gctttccggt ctgttctgtg ggctgcgcgg taaagcgcaa attggtaaag gcatgtgggc
     7981 aatgccggac ctgatggcag acatgtacag ccagaagggc gaccaactgc gtgccggggc
     8041 aaacacagcc tgggttccgt caccaaccgc tgctacgctc catgcgctgc actaccatca
     8101 aaccaacgta cagagcgtgc aagccaacat tgcccagacc gagttcaatg ctgaatttga
     8161 accgctgctg gacgatctgc tgactattcc ggttgctgaa aacgctaact ggtcggcgca
     8221 agagatccaa caagagctgg ataacaacgt gcaggggatt ctggggtacg tggtgcgctg
     8281 ggtggagcag gggattggtt gttcaaaagt gccggatatt cacaatgtgg cgctgatgga
     8341 agaccgcgca acgctgcgta tctccagcca gcatatcgcc aactggttac gtcacggtat
     8401 tctgaccaaa gaacaggtgc aggcgtcgct ggagaatatg gcgaaagtgg ttgatcagca
     8461 aaacgctggc gatccggctt atcgtccgat ggcggggaat ttcgctaact cgtgtgcttt
     8521 taaagctgcc agcgatttga tcttcctcgg cgtgaaacag ccaaacggtt ataccgaacc
     8581 attattacac gcctggcgtt tacgcgaaaa agaaagtcat taatagcgtc aaaaaagccc
     8641 cggcaagttt tattgtcggg gctatgatag taactgcggt agtacattta tcccggaaat
     8701 aaccggagcg attatcaggg tttaacaata ttaaataccc gtagcgcatt actagcgggc
     8761 ctaaaacaac aatatggaca tcttaacgat actctgcgcc tgcgtgtgcg gaacagaaac
     8821 ccttacccga tatacttgtt atgccagagg tagcaattgt gttagcggcg tactgttgct
     8881 gtaaagtata acgcctatat tttacagttc agtcctcttt tgcttattac aaccttaagt
     8941 atatcaagca tataaagata ataagagact gaacaatatg gttacctgga cccaaatgta
     9001 tatgccgatg ggaggactgg ggctatccgc tctggtcgcc ctgatcccga taatattctt
     9061 cttcgttgca ctcgcggtat tacgtctgaa aggacatgtc gctggagcaa taacccttat
     9121 attatctatc ctgattgcga tattcgcctt taaaatgccg attgatatgg catttgctgc
     9181 tgcgggctat ggctttattt atggattatg gccaattgcg tggattattg tcgcggcggt
     9241 gttcctctat aaattaaccg ttgccagcgg acagttcgat attatccgca gctcggttat
     9301 ctccatcacc gacgatcagc gtttgcaggt gttactgatt ggtttctcct ttggtgcgtt
     9361 gctggaagga gcggctggct ttggtgcgcc ggtggcgatt accggtgcgc tgctggtggg
     9421 cctgggcttc aaaccgttat acgcggcggg gctgtgtctg attgccaata ctgcgccggt
     9481 ggcgtttggt gcgttgggcg tgccgattct ggtcgccggt caggtaacgg gaatcgatcc
     9541 gttccacatt ggcgcaatgg cgggacgtca gttaccgttc ctgtcggttc ttgtgccgtt
     9601 ctggctggta gcaatgatgg acggctggaa aggggtgaaa gagacgtggc cagcggcgct
     9661 ggttgctggg ggaagcttcg ctgtcactca gttctttacc tctaattata ttggtccgga
     9721 actgccggat attacttcgg cgctggtgag tatcgtctca ctcgctttat tccttaaagt
     9781 ctggcggccg aaaaataccg aaacggcaat cagcatggga caatccgcag gtgcgatggt
     9841 ggtaaataag ccatcttctg gcggtcccgt gccttcagaa tatagtctgg ggcaaatcat
     9901 tcgagcgtgg tcaccgtttt taatcttaac ggtgctggtc accatctgga ccatgaagcc
     9961 gtttaaagcg ttatttgctc cgggcggcgc gttttattca ctggtgatta atttccagat
    10021 ccctcatttg catcaacaag tgttgaaagc ggcacccatt gtcgcccaac caacgccaat
    10081 ggatgcggtg tttaaattcg accccctctc ggctggcggc accgctattt ttattgcggc
    10141 gattatctct atcttcatcc tcggcgtggg catcaagaaa ggtattggcg tctttgccga
    10201 aacgctaatt agcttgaagt ggccgatact gtcgattggc atggtgctgg cgttcgcctt
    10261 cgtcaccaac tattctggca tgtccaccac gctggcgctg gtactggcag gtacaggcgt
    10321 gatgttcccg ttcttctcac cgttcctcgg ctggctgggc gtattcctta ccggctcgga
    10381 cacctcctct aacgccctgt ttggttcact gcaatcgacc acggcgcagc aaatcaacgt
    10441 ctctgacact ctgctggtgg cagcaaatac cagcggcggc gtaactggca agatgatctc
    10501 cccgcaatct atcgccgtgg cctgcgccgc gacgggcatg gtgggccgag aatctgaact
    10561 gttccgctac accgtgaagc acagtctgat ttttgccagc gttatcggca ttatcacgct
    10621 gttgcaggcg tatgtgttta ccgggatgtt agtctcgtaa atcaataaag ccggatgatc
    10681 gttaatatca tccggcttca tttacattat tctgtcttat taattgaaaa tcatttctcg
    10741 ctttattatc catgcagcaa taatgttttt cgttcagata acctgctaaa taattaagag
    10801 cgtgagaaca tctatttttt tatcacgcaa aacaagtctg tattaattat gttcgcttga
    10861 tgagcaaaca ccgttctatc tgttgctttt gcaatcttaa tcgttaaaac taccaacgaa
    10921 tttatata
//
LOCUS       DAPGOF010000167        10870 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.168, whole genome shotgun sequence.
ACCESSION   DAPGOF010000167 DAPGOF010000000
VERSION     DAPGOF010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.168"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            63..824
                     /gene="adiY"
                     /locus_tag="RHV13_003827"
     CDS             63..824
                     /gene="adiY"
                     /locus_tag="RHV13_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HDV1243305.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            961..2298
                     /gene="adiC"
                     /locus_tag="RHV13_003828"
     CDS             961..2298
                     /gene="adiC"
                     /locus_tag="RHV13_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HDV1243306.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDVPISKD"
     gene            2402..4045
                     /gene="eptA"
                     /locus_tag="RHV13_003829"
     CDS             2402..4045
                     /gene="eptA"
                     /locus_tag="RHV13_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HDV1243307.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCSKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4042..4710
                     /gene="basR"
                     /locus_tag="RHV13_003830"
     CDS             4042..4710
                     /gene="basR"
                     /locus_tag="RHV13_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HDV1243308.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4711..5811
                     /gene="pmrB"
                     /locus_tag="RHV13_003831"
     CDS             4711..5811
                     /gene="pmrB"
                     /locus_tag="RHV13_003831"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HDV1243309.1"
                     /translation="MNLMHFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDSGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWIRLKKDQYVANQI"
     gene            complement(5787..5876)
                     /gene="pmrR"
                     /locus_tag="RHV13_003832"
     CDS             complement(5787..5876)
                     /gene="pmrR"
                     /locus_tag="RHV13_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HDV1243310.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(5988..7490)
                     /gene="proP"
                     /locus_tag="RHV13_003833"
     CDS             complement(5988..7490)
                     /gene="proP"
                     /locus_tag="RHV13_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HDV1243311.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7754..8632)
                     /locus_tag="RHV13_003834"
     CDS             complement(7754..8632)
                     /locus_tag="RHV13_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HDV1243312.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRHHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTIPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLA"
     gene            complement(8629..10857)
                     /gene="crfC"
                     /locus_tag="RHV13_003835"
     CDS             complement(8629..10857)
                     /gene="crfC"
                     /locus_tag="RHV13_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HDV1243313.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQRAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLDSYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGYITPQQIFPVS
                     SMWGYLANRARYELANNGKLPPPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLFALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIKLALTSANHFLRQQQDAVNAQLAALFQD
                     DSESLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVLHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2715 a         2848 c         2681 g         2626 t
ORIGIN      
        1 tttcccggta tcgctttgct ttagcgagaa ctggtctttt atttgcactc aggagtacat
       61 gtatgaggat ttgcagcgac caaccttgta ttgttttatt gactgaaaaa gatgtctgga
      121 taagggtgaa tgggaaagaa cctattagcc ttaaagctaa ccatatggcg ttattaaatt
      181 gtgaaaataa tattatcgac gtctcctctc ttaacaacac tttggttgct catattagtc
      241 acgacatcat caaagattac ctccggtttc tgaataaaga tctctcgcaa ataccagtat
      301 ggcaacgtag cgctacgccc atactcactc tgccatgcct gacgccagac gtctttcgcg
      361 ttgccgcgca acacagcatg atgcccgcag aaactgagtc agaaaaggaa cgaacacgcg
      421 cattattatt cacggtgcta tcccgttttc tcgacagtaa aaaattcctt tcattaatga
      481 tgtatatgtt acgtaattgt gtaagtgaca gcgtttatca aattattgaa agcgatattc
      541 acaaagactg gaatcttagt atggtagcca gttgtttatg tcttagccca agtctgttaa
      601 agaaaaagct gaaaagcgaa aacaccagtt atagccaaat aatcaccacc tgccgcatgc
      661 gttatgccgt aaatgaatta atgatggacg gtaaaaatat ctctcaggta tcacagtcct
      721 gcggctacaa cagtacgtcg tactttattt ctgtctttaa agacttctac ggtatgacgc
      781 cgctgcatta tgttagtcag cacagagaac gcactgtcgc ctgattttta accttaacga
      841 agagctatat taataacggc atcagcgata acccggtcga taataattca actatcgaat
      901 gcaggcgtat gatatgacgt aatttattgt cacgaagctc gccttcgcag gagtttaatt
      961 atgtcttcgg atgctgatgc tcacaaagtg ggcttaatcc ccgtcaccct gatggtgtcg
     1021 gggaatatta tggggtcagg tgtttttctg ttacctgcaa acctggcctc tactggcggg
     1081 attgccattt atggatggtt ggtgacgatt atcggtgcac tggggctctc gatggtatac
     1141 gccaaaatgt cgttcctcga cccaagtcct ggtggttctt acgcttacgc ccgccgctgc
     1201 tttggcccgt ttctcggtta tcaaaccaac gtcctctact ggctggcctg ctggatcggc
     1261 aatatcgcca tggtggtcat tggcgtagga tatttaagtt acttcttccc gattctgaaa
     1321 gatccattgg tattaaccat cacctgcgtc gtggtgctgt ggatcttcgt cctgctgaac
     1381 attgtcggtc cgaaaatgat cacccgtgtg caggcagttg ccaccgtgct ggcgctgatc
     1441 cccatcgtcg ggattgccgt atttggctgg ttctggttcc gtggtgaaac ctatatggcg
     1501 gcatggaacg tcagcggcct gggcaccttc ggtgcaattc aaagtaccct taacgttacg
     1561 ctgtggtcgt tcatcggtgt ggaaagtgcc tccgttgccg caggtgtggt gaaaaacccg
     1621 aaacgcaatg tccctatcgc caccattggt ggggtattga ttgccgccgt ttgctatgta
     1681 ctttctacca ccgcgattat ggggatgatc cctaatgccg cactgcgcgt ttctgcttcg
     1741 ccattcggtg atgccgcacg gatggcgttg ggtgacaccg ccggggccat tgtttccttc
     1801 tgcgcagctg cgggttgctt aggttcactg ggcggctgga cgttgctggc gggtcaaacg
     1861 gcgaaagccg ctgccgatga cggactgttc ccaccgattt ttgcccgtgt aaataaagcg
     1921 ggcacgccag tggcggggct gattatcgtc ggtattttga tgaccatctt ccagctcagc
     1981 agcatttcac caaacgcgac caaagagttc ggtctggttt cttccgtctc ggtcatcttt
     2041 acactggtgc catatcttta cacctgtgcg gcgttactgc tgctcggtca cggtcacttt
     2101 ggtaaagcac gcccggcata tctggctgtt actaccattg ccttcctcta ctgcatttgg
     2161 gccgtggtgg ggtccggggc gaaagaggtt atgtggtcat ttgtcaccct gatggtaatc
     2221 accgccatgt atgcgctgaa ttacaaccgg ctgcataaaa acccgtatcc cttagatgta
     2281 ccaataagca aagattaatt ccccttaatc cagcaaacat aaaagccaac cttaagaact
     2341 taaggttggc ttaattttgc tttgcgagca tatgcgcact ttgttcgatg gaaacaccgt
     2401 gatgttgaag cgcctactaa aaagaccctc tttgaattta ctcgcctggc tattgttggc
     2461 cgctttttat atctctatct gcctgaatat tgcctttttt aaacaggtgt tgcaggcact
     2521 gccgctggat tcgctgcata acgtactggt tttcttgtcg atgccggtcg tcgctttcag
     2581 cgtgattaat attgtcctga cactaagctc tttcttatgg cttaatcgac cactggcctg
     2641 cctgtttatt ctggttggcg cggctgcaca atatttcata atgacttacg gcatcgtcat
     2701 cgaccgctcg atgattgcca atattattga taccactccg gcagaaagtt atgcgctgat
     2761 gacaccgcaa atgttattaa cgctgggatt cagcggcgtg cttgctgcgc tgattgcctg
     2821 ctggataaaa atcaaacctg ccacctcgcg tctgcgcagt gttcttttcc gtggagccaa
     2881 tattctggtt tctgtactac tgattttgct ggtcgccgca ctgttttata aagactacgc
     2941 ctcgttgttc cgcaataaca aagagctggt gaaatcctta agcccctcta acagcattgt
     3001 tgccagctgg tcatggtact cccatcagcg actggcaaat ctaccgctgg tgcgaattgg
     3061 tgaagacgcc caccgcaacc cgttaatgca gaacgaaaaa cgtaaaaatt tgaccatcct
     3121 gattgtcggc gaaacctcgc gggcggagaa cttctccctc aacggctacc cgcgtgaaac
     3181 taacccgcgg ctggcgaaag ataacgtggt ctatttccct aataccgcat cttgcggcac
     3241 ggcaacggca gtttcagtac cgtgcatgtt ctcggatatg ccgcgtgagc attacaaaga
     3301 agagctggca cagcaccagg aaggcgtgct ggatatcatt cagcgagcgg gcatcaacgt
     3361 gctgtggaat gacaacgatg gcggctgtaa aggtgcctgc gaccgcgtgc ctcaccagaa
     3421 cgtcaccgcg ctgaatctgc ctggtcagtg catcaacggc gaatgctatg acgaagtgct
     3481 gttccacggg ctggaagagt acatcaataa cctgcagggt gatggcgtga ttgtcttaca
     3541 caccatcggc agccacggtc cgacctatta caaccgctat ccgccgcagt tcaggaaatt
     3601 taccccaacc tgcgacacca atgagatcca gacctgtagc aaagagcaac tggtgaacac
     3661 ttacgacaac acgctggttt acgtcgacta tattgttgat aaagcgatta atctgctgaa
     3721 agaacatcag gataaattta ccaccagcct ggtttatctt tctgaccacg gtgaatcgtt
     3781 aggtgaaaat ggcatctatc tgcacggtct gccttatgcc atcgccccgg atagccaaaa
     3841 acaggtgccg atgctgctgt ggctgtcgga ggattatcaa aaacggtatc aggttgacca
     3901 gaactgcctg caaaaacagg cgcaaacgca acactattca caagacaatt tattctccac
     3961 gctattggga ttaactggcg ttgagacgaa gtattaccag gccgcggatg atattctgca
     4021 aacttgcagg agagtgagtg aatgaaaatt ctgattgttg aagacgatac gctgttattg
     4081 cagggactga ttctggcggc gcaaaccgaa ggctacgcgt gcgatggcgt gacaaccgcg
     4141 cggatggcgg aacaaagcct tgaggccggt cattacagcc tggtggtact ggatttaggg
     4201 ttacccgacg aagatggact gcattttctc gcccgtatcc ggcagaaaaa atataccctg
     4261 ccggtactga tcctcaccgc tcgcgatacg ctgaccgaca aaatcgccgg gctggatgtc
     4321 ggtgccgatg actatctggt gaagcctttt gcgctggaag agttacacgc ccgtatccgc
     4381 gccctgctac gacgccataa taatcagggc gaaagtgagc tgattgttgg caatctgacg
     4441 ctgaatatgg gtcgccgtca ggtatggatg ggcggtgaag agttgattct gacgcccaaa
     4501 gaatatgctc tgctgtcacg gttaatgctc aaagcaggca gtccggtgca tcgggaaatt
     4561 ctctacaacg acatctataa ctgggacaat gaaccctcga ccaacaccct ggaagtgcat
     4621 atccacaatc tgcgcgacaa agtgggcaaa gcccgtatcc gcaccgtgcg cggctttggc
     4681 tatatgctgg tcgcgaatga ggaaaactaa ttgaatctga tgcattttct gcgccgacca
     4741 atatcgctgc gccaacggct gatattgacc atcggggcca ttttgttggt gtttgagctg
     4801 atcagcgtct tctggctatg gcatgaaagt accgagcaga ttcagctgtt tgagcaggcg
     4861 ctacgcgaca atcgcaacaa cgatcgccac atcatgcgtg agatccgcga ggcggtcgcc
     4921 agcctgattg tccccggcgt ctttatggtc agcctgacgc tatttatctg ctatcaggcg
     4981 gtacgccgca tcacccgccc gctggcggag ctgcaaaaag agctggaagc gcgcactgcc
     5041 gacaacttaa cgcccattgc cattcacagc gccaccctcg aaatcgaagc ggtggtttcg
     5101 gcgttaaacg atctggtcag tcgcctgacc agcacgctgg ataacgaaag gttgtttacc
     5161 gctgacgtcg cgcacgaact gcgaacgcca ctggcggggg tgcgtttgca tctggaactg
     5221 ctggcgaaaa cgcatcacat tgatgtagca ccgttagtgg cacggcttga tcagatgatg
     5281 gagagcgtct cccaactgct gcaactggcg cgtgccggac agtcattttc ttccggtaat
     5341 tatcaacatg taaaactgct ggaagatgtc attctcccct cgtatgacga actcagtacc
     5401 atgctcgacc agcgacagca aaccctgcta ttgccagaga gcgccgccga catcaccgtt
     5461 cagggtgatg caaccctgct gcggatgtta ttgcgaaacc tggtagaaaa cgcccatcgt
     5521 tacagcccgc aaggcagcaa cattatgatt aagctgcaag aagatagcgg agcggtcatg
     5581 gccgttgaag atgaaggacc gggtattgat gagagtaaat gcggggagtt gagtaaagcg
     5641 tttgtacgta tggacagccg ttatggcggg attggtctgg ggttaagtat tgtcagccgc
     5701 attacacagt tgcatcacgg gcagtttttc ctgcaaaacc ggcaagagac ttctggcacg
     5761 cgcgcctgga tacggctgaa gaaagatcag tacgtggcaa accagatata aagaaagctg
     5821 ctgaccacca gcacgctgaa cacggtagtt aaactttcat aaacacggtt tttcattgca
     5881 ctctcctccg ttaatctgga ggagagtatg cgcggcagag attaagtgaa ccttaagagt
     5941 tcaattggcc tgataagaca gcgtcacatc aggccatccg tttcagctta ttcatcaatt
     6001 cgcggatgtt gctgcaccag gcgggtacgt ttcgcctgca aatcggcaat ctcgtggtca
     6061 atatcatcga ttttctgctc gatattatcg taatgctcga cgagaatttc cttcgcttcc
     6121 tgtatatctg acgccgccgg tgtcgcacct ttcaacggac gatttgccgt ctctttcatg
     6181 gttacgccgg tgattaaacc aatcaccgcc actaccatca ggtaataggc tggcatcatc
     6241 agattctgcg agctttcgac cagccaggcc gccagtgttg gcgtcagacc ggcaaccagc
     6301 accgaaatat taaatgccgc cgccagcgcg ctgtaacgga tatgcgtcgg gaacatcgct
     6361 ggcaaggtag aggccataac gcccgtaaag cagttaagga tcaccgccag catcagtaac
     6421 ccggcaaaaa tcaggccgat gacgttactg ttaatcagaa taaacgccgg gatcgccaac
     6481 acaaacagtg caacactacc aagtagcaca aacggacgac ggccaaaacg gtcactcagc
     6541 aagcccatca ccggctggac aaacagcata ccgatcataa tggcgataat aatcagcacc
     6601 ccgtggtctt cggagtaatg caggttatgc gacaaataac tcggcatata ggtcagcaac
     6661 atgtagtaag tcacgttggt ggcaattacc agaccaatac atgtcaacag gctgcgccag
     6721 tatttagtgg caatctcttt aaacgagact ttcgggccat cctgcaaacc ttcgcggtcg
     6781 ccctgttcca gtttatcaac atgctgctgg aacgccggag tctcttccag cgcatggcgc
     6841 aggtaaagcc cgataatccc taacggcaga gcaataaaga acggaatacg ccagccccag
     6901 tcgaggaagt tcgcttcgcc gacaatggtc gaaattaaca ccaccacgcc cgcacccagc
     6961 acaaacccgg caatagaacc gaagtccagc cagctgccca taaatccacg tttacggtca
     7021 ggggagtatt ccgcaacaaa tatcgacgcc ccggtatatt caccgccgac cgagaaacct
     7081 tgtgccatct tacagatcaa cagcagaatc ggtgcccaaa taccaatcgt gtcgtaggac
     7141 ggtattaagc caatacagaa cgtactgatc gacataatca caatagtgat agcgaggatc
     7201 ttctggcgac catatttatc gcccaacata ccaaagaaga gcccgccaag cggtcgaatc
     7261 agaaagggaa cggagaacgt ggcaagtgca gcaaccatct gcacgctggg gtcagccccc
     7321 gggaaaaaaa ctttacctaa tgcgtaagca acaaaaccat aaacaccaaa atcgaaccat
     7381 tccattgcat tacccagtga tgctgcggta atggctttac gcagtttacc gtcatcaata
     7441 atggtgacat cacgaagggt aatcggtttt acttttttcc ttttcagcat agctttcctc
     7501 gcagagttgg cccatttccg caaacgccag aaaaatcagc gcgacggccg caaaagggga
     7561 gcgggaattg caggatgcaa tctctgtaac aagcgtagcg ggtttgctta caccctccgg
     7621 ttaaggaatg tacaatcaaa ccagttgacg cctgaattgg gcagttaatg acctttttac
     7681 cctaccagtg tttaaatttg tgatcaactt cacacatgga tttcgtttag gactcattga
     7741 tgtaactgat tatttacgcc aaatttacag agaagatttc ctcgaccatt gcccccgcca
     7801 gacgctgcgc tgaacagagt cgtggcatcc ccagcgcgac agtttgccaa cgttgggtca
     7861 ctgaccagca aacgggttgt ttatctgccg tggtccactt tcccagccaa cttaaaaaat
     7921 cagcatgatc cccgcccgtc ggaatggtcg aagaggcaaa ccactgatgg taatagtgac
     7981 gcgtggtcgg ggccgcattg atactctctg ccagatgttg tgtcgctttc acgcgcaaat
     8041 cacccagcaa caacgccacc gcttgcgggc ttcccagacg acaggcatcg ccaaatgccc
     8101 cccaacgcaa ctcttcgaga acgataaaac agcgcccagg caacgaccag gcgttatacg
     8161 ccccagcacg ccagcgggta aaaatttgct cgctctgttc acccgcctgc accagtaggc
     8221 ctcgttgcgt taacgccttt tccttccagc gacaacgggc tgaaaaccac tcatacaagg
     8281 cggcaatttg ctgagggagc tccgggaagg cagagtgatg gcggtgatcc attttttgca
     8341 gcagcgccag atgctgcgcg atctgcgtca gtgccagatg accgggatcc agcatcgacg
     8401 ccagtttttc caccaatgtc aaacgcaggc taagcggcgc atttaacatt ccgccgttcg
     8461 tccatgtctg aagctgcacg cgcgcaagaa gttcctgcat cagtcgttca cgaaactgct
     8521 gctgatgggc ttgtggaagg cgggcatcat cgccctgcgc cagatccact aaaaagcggg
     8581 gataaaccga ctccagcacg cgaccggggc cgtcaagtaa cgtcttggtc aatatcgttc
     8641 tgccgtgaaa agtgtttgaa tatcatcgcg taacagctgg gcgtcggtgt aaatccagcc
     8701 gtgagtcatc acagtctgct gcaattgctg ctgcatcagc ctgaccaccg attcattttg
     8761 ttgacgcaga gccagacttt cgcgtaaacg cgtctgtaat tccgtcaaac atgaagcgaa
     8821 ctcagcgaaa aaagtattca tgcctgccgt aacagattca tcgacctgct ctgccagaac
     8881 tttagccatt tgttggcaat agagatcgac ttctgcgctt aatgctcgtt gcaacacact
     8941 gtaatcaacc gtttctgtcg gggatttctc atttccccgt ccccagtcgg gctgattcaa
     9001 ccagcgcgaa aaagtctcac gcacaacgcc taaacgcgtg ctctgctcgt ccgttgcttc
     9061 ctggcgcgaa atgacttcac tgaacagctg gcgagtgttg aagtggagaa ctacgccgtg
     9121 aaaaacagga aaatgaaacc caggacgaaa ccctgactcg ctcaattcca ttttgacttg
     9181 ttgctcaatg gggcgaataa catcggttaa cacccggcaa agggtggatt ccagctcggc
     9241 aaaacgcagc gtaaagtcgc gactgatggt gttctgcgcc gtctgtaaca gtgtctcaca
     9301 gcgggtacga atctcgctta acgactccga atcatcctga aacaaggcgg ctaactgcgc
     9361 attcaccgca tcttgctgtt ggcgcagaaa gtggttggcg gaggtcaggg ccagcttgat
     9421 ttcatgttta atctcgccgc tcacctgcgc ctgattgagt tgcaatagct gcaaactttc
     9481 ttcgacctga tggatatttt gccgcaattg ttcacaagcg acgtttaacc cgtgcgcacg
     9541 aaaatccagg tattcccgcg cctgctgcgc gtaattcaac agtttatgcg cagcagatcg
     9601 cagagcaaac aacgaggcgt tagcgtaagc ggcatgaagc aacgcctgaa ttggctgggc
     9661 gaacagcgaa tcttcccaca actgatcggc agcatgacga atatgttcga ggtccgccag
     9721 atcggcatga cgccagcgcc tgccgagcgc ggcatgggca aaatcttcca cccagcgttg
     9781 ttgctctggc ggtggtaact taccgttgtt ggctaactca tagcgcgccc gattcgccag
     9841 gtagccccac atcgacgaca ccggaaatat ctgctgtggc gtaatatagc ctttcatcag
     9901 cgtcccggaa atcagtgccc gcacctggtc ggcgtcgtca ctgttacgat cctgttgatc
     9961 gaacttattg accagcacat acagcggcac cgattgcccc accgccaaaa tcgcctcacg
    10021 gacctcttca tcggagatcg atttcagttg cgtataatcc agcaccgcca gtaccgccga
    10081 ggcgcgtgcc agctgctggt taagcatttt ttgcagatgc ggttgcccgg cttcatttgg
    10141 cccgggggta tccagtaacg tcaactgacc gggataactg tccagccccg ccagatggac
    10201 aaactccact tcaatcacgg gaatatgctc aatggcggcg taagcagaaa aaggaaaatc
    10261 gacgtccagc gccttcgcca gtcgcactaa atcattcaaa cttttcagac aatgaaaaat
    10321 aggctgggca cccagataat atttttcgaa agcgacgcca ttttcgatcc gctgcataag
    10381 cgcacgcata tctttatcta tttccagcac atcggtcaga tgcttaatat cgcaatcacg
    10441 caggcgctgt tgtaattgtt gaattaaaca atcgattggc gcgacatgtg aaaaatgcag
    10501 taccggttcc ttttgcccgg gcgtatggcg aataagcgtc ggcagcgcag tcattgggcg
    10561 attacgatta ggcagaacct ccgtaccaac aatggcatta atggtggttg atttccctgc
    10621 tttcatggta ccgacaattg ccagcaccat ttccagtcgg gaaattttac gcaactcatt
    10681 attcagcatc gcatgacgct cggcgatatt tggctgactc cagggtaaag cccgttgtgg
    10741 cgcatcgtct ccgggtacag agagaggcat tttttccagt aactgcaact gttggcgaga
    10801 aagctgtaac aggcgttcag cctcctgact taactcatac agggtctgtg tgtacataga
    10861 aaattcttcc
//
LOCUS       DAPGOF010000168        10861 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.169, whole genome shotgun sequence.
ACCESSION   DAPGOF010000168 DAPGOF010000000
VERSION     DAPGOF010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10861
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.169"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            116..2161
                     /gene="paaZ"
                     /locus_tag="RHV13_003836"
     CDS             116..2161
                     /gene="paaZ"
                     /locus_tag="RHV13_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HDV1243314.1"
                     /translation="MQQLASFLSGTWQSGRGRSRLIHHAISGEALWEVTSEGLDMAAA
                     RQFAIEKGAPALRAMTFIERAAMLKAVAKHLLSEKERFYALSAQTGATRADSWVDIEG
                     GIGTLFTYASLGSRELPDDTLWPEDELIPLSKEGGFAARHVLTSKSGVAVHINAFNFP
                     CWGMLEKLAPTWLGGMPAIIKPATATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDL
                     LDHLDSQDVVTFTGSAATGQMLRVQPNIVAKSIPFTMEADSLNCCVLGEDVTPDQPEF
                     ALFIREIVREMTTKAGQKCTAIRRIIVPQALVNAVSDALVARLQKVVVGDPAQEGVKM
                     GALVNAEQRADVQEKVNILLAAGCEIRLGGQADLSAAGAFFPPTLLYCPQPDETPAVH
                     ATEAFGPVATLMPAQNQQHALQLACAGGGSLAGTLVTADPQIARQFIADAARTHGRIQ
                     ILNEESAKESTGHGSPLPQLVHGGPGRAGGGEELGGLRAVKHYMQRTAVQGSPSMLAA
                     ISKQWVRGAKVEEDRIHPFRKYFEELQPGDSLLTPRRTMTEADIVNFACLSGDHFYAH
                     MDKIAAAESIFGERVVHGYFVLSAAAGLFVDAGVGPVIANYGLENLRFIEPVKPGDTI
                     QVRLTCKRKTLKKQRSAEEKPTGVVEWAVEVFNQHQTPVALYSILTLVARQHGDFVD"
     gene            2409..4682
                     /gene="tynA"
                     /locus_tag="RHV13_003837"
     CDS             2409..4682
                     /gene="tynA"
                     /locus_tag="RHV13_003837"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HDV1243315.1"
                     /translation="MGSPSLYSARKTTLALAVALSFAWQAPVFAHGGEAHMVPMDKTL
                     KEFGADVQWDDYAQLFTLIKDGAYVKVKPGAQTAIVNGQPLALQVPVVMKDNKAWVSD
                     TFINDVFQSGLDQTFQVEKRPHPLNALTADEIKQAVEIVKASADFKPNTRFTEISLLP
                     PDKEAVWAFALENKPVEQPRKADVIMLDGKHIIEAVVDLQNNKLLSWQPIKDAHGMVL
                     LDDFASVQNIINNSEEFTAAVKKRGITDAKKVITTPLTVGYFDGKDGLKQDARLLKVI
                     SYLDVGDGNYWAHPIENLVAVVDLEQKKIVKIEEGPVVPVPMTARPFDGRDRVAPAVK
                     PMQIIEPEGKNYTITGDMIHWRNWDFHLSMNSRVGPMISTVTYNDNGTKRKVMYEGSL
                     GGMIVPYGDPDIGWYFKAYLDSGDYGMGTLTSPIARGKDAPSNAVLLNETIADYTGVP
                     MEIPRAIAVFERYAGPEYKHQEMGQPNVSTERRELVVRWISTVGNYDYIFDWIFHENG
                     TIGIDAGATGIEAVKGVKAKTMHDETAKDDTRYGTLIDHNIVGTTHQHIYNFRLDLDV
                     DGENNSLVAMDPVVKPNTAGGPRTSTMQVNQYNIGNEQDAAQKFDPGTIRLLSNPNKE
                     NRMGNPVSYQIIPYAGGTHPVAKGAQFAPDEWIYHRLSFMDKQLWVTRYHPGERFPEG
                     KYPNRSNHDTGLGQYSKDNESLDNTDAVVWMTTGTTHVARAEEWPIMPTEWVHTLLKP
                     WNFFDETPTLGALKKDK"
     gene            complement(4740..6239)
                     /gene="feaB"
                     /locus_tag="RHV13_003838"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
     CDS             complement(4740..6239)
                     /gene="feaB"
                     /locus_tag="RHV13_003838"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /EC_number="1.2.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetaldehyde dehydrogenase"
                     /protein_id="HDV1243316.1"
                     /translation="MTEPHVAVLSQVQQFLDRQHGLYIDGRPGPAQSEKRLAIFDPAT
                     GQEIASTADANEADVDNAVMSAWRAFVSRRWAGRLPAERERILLRFADLVEQHSEELA
                     QLETLEQGKSIAISRAFEVGCTLNWMRYTAGLTTKIAGKTLDLSIPLPQGARYQAWTR
                     KEPVGVVAGIVPWNFPLMIGMWKVMPALAAGCSIVIKPSETTPLTMLRVAELASEAGI
                     PDGVFNVVTGSGAVCGAALTSHPHVAKISFTGSTATGKGIARTAADHLTRVTLELGGK
                     NPAIVLKDADPQWVIEGLMTGSFLNQGQVCAASSRIYIEAPLFDTLVSGFEQAVKSLQ
                     VGPGMSPVAQINPLVSRAHCDKVCSFLDDAQAQQAELIRGSNGPAGEGYYVAPTLVVN
                     PDAKLRLTREEVFGPVVNLVRVADGEEALQLANDTEYGLTASVWTQNLSQALEYSDRL
                     QAGTVWVNSHTLIDANLPFGGMKQSGTGRDFGPDWLDGWCETKSVCVRY"
     gene            6475..7380
                     /gene="feaR"
                     /locus_tag="RHV13_003839"
     CDS             6475..7380
                     /gene="feaR"
                     /locus_tag="RHV13_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HDV1243317.1"
                     /translation="MNPAVDNEFQQWLSQINQVCGNFTGRLLTERYTGVLDTHFAKGL
                     KLSTVTTNGVNLYRTWQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQISAGDITLLDA
                     SRPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMN
                     NPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEW
                     IAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(7552..7881)
                     /locus_tag="RHV13_003840"
     CDS             complement(7552..7881)
                     /locus_tag="RHV13_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HDV1243318.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(7886..8071)
                     /locus_tag="RHV13_003841"
     CDS             complement(7886..8071)
                     /locus_tag="RHV13_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HDV1243319.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(8068..10707)
                     /locus_tag="RHV13_003842"
     CDS             complement(8068..10707)
                     /locus_tag="RHV13_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HDV1243320.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQANLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTNINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
BASE COUNT         2721 a         2808 c         2675 g         2657 t
ORIGIN      
        1 tttagtagtt gatatacaga tcgcgcagtt aacatttcgt taaaatacgc cttgcttttt
       61 atgattcgtg atttaactat tagcaacaga aatgtgaaac atctggagag tagcgatgca
      121 gcagttagcc agtttcttat ccggtacctg gcagtctggc cggggccgta gccgtttgat
      181 tcatcacgct attagcggcg aggcgttatg ggaagtgacc agtgaaggtc ttgatatggc
      241 ggctgcccgc cagtttgcca ttgaaaaagg tgcccccgcc ctccgcgcga tgacctttat
      301 cgaacgtgcg gcgatgctta aagcggtcgc taaacatctg ctgagtgaaa aagagcgctt
      361 ctatgctctt tctgcgcaaa caggcgcaac gcgggcagac agttgggttg atattgaagg
      421 tggcattggg acgttattta cttacgccag cctcggtagc cgggagctgc ctgacgatac
      481 gctgtggccg gaagatgaat tgatcccctt atcgaaagaa ggtggatttg ccgcgcgcca
      541 tgtactgacc tcaaagtcag gcgtggcagt gcatattaac gcctttaact tcccctgctg
      601 gggaatgctg gaaaagctgg caccaacgtg gctgggcgga atgccagcca tcatcaaacc
      661 agctaccgcg acggcccaac tgactcaggc gatggtgaaa tcaattgtcg atagtggtct
      721 tgttcccgaa ggcgcaatta gtctgatctg cggtagtgct ggcgacttgt tggatcatct
      781 ggacagccag gatgtggtga ctttcacggg gtcagcggcg accggacaga tgctgcgagt
      841 tcagccaaat atcgtcgcca aatctatccc cttcactatg gaagctgatt ccctgaactg
      901 ctgcgtactg ggcgaagatg tcaccccgga tcaaccggag tttgcgctgt ttattcgtga
      961 aattgtgcgt gagatgacca caaaagccgg gcaaaaatgt acggcaatcc ggcggattat
     1021 tgtgccgcag gcattggtta atgctgtcag tgatgctctg gttgcgcgat tacagaaagt
     1081 cgtggtcggt gatcctgctc aggaaggcgt gaaaatgggc gcactggtaa atgctgagca
     1141 gcgtgccgat gtgcaggaaa aagtgaacat attgctggct gcaggatgcg agattcgcct
     1201 cggtggtcag gcggatttat ctgctgcggg tgccttcttc ccgccaacct tattgtactg
     1261 tccgcagccg gatgaaacac cggcggtaca tgcaacagaa gcctttggcc ctgtcgcaac
     1321 gctgatgcct gcacaaaacc agcaacatgc tctgcaactg gcttgtgcag gtggcggtag
     1381 tcttgcggga acgctggtga cggctgatcc gcaaattgcg cgtcagttta ttgccgacgc
     1441 ggcgcgtacg catgggcgaa ttcagatcct caatgaagag tcggcaaaag aatccaccgg
     1501 gcatggctcc ccactgccac aactggtaca tggtgggcct ggtcgcgcag gaggcggtga
     1561 agaattaggt ggtttacgag cggtaaaaca ttacatgcag cgaaccgctg tacagggtag
     1621 tccgtcgatg cttgccgcta tcagtaaaca gtgggtgcgt ggtgcgaaag tcgaagaaga
     1681 tcgtattcat ccgttccgca aatattttga ggagctgcaa ccaggcgaca gcctgctgac
     1741 tccccgccgc acaatgacag aggccgatat tgttaacttt gcttgcctca gcggcgatca
     1801 tttctatgcg catatggata agattgctgc tgccgaatct attttcggtg agcgtgtggt
     1861 gcatgggtat tttgtgcttt ctgcggctgc gggtctgttt gtcgatgccg gagttggtcc
     1921 ggtcattgct aactacgggc tggaaaactt gcgttttatc gagccagtaa aaccaggcga
     1981 taccatccag gtgcgtctca cctgtaagcg caagacgctg aaaaaacagc gtagcgcaga
     2041 agaaaaacca acaggtgtgg tggaatgggc ggtagaggta ttcaatcagc atcaaacccc
     2101 ggtggcgctg tattcaattc tgacgctggt ggccaggcag cacggtgatt ttgtcgatta
     2161 atcggtgaat gaagggcaac ggcgaatagt tgccctttta tttcaccaaa ttttgtgacg
     2221 ttgtcacgtt atgcatgatg tgtatctgta ttttcagggc acccactgta tgaaaagctg
     2281 gcacacctgc caaacaacct ggcaggtgca ggcaatcccc tttacatcag tactgataat
     2341 gtgaacctga ctaaaccgcc cacagagcgc ggttgctaac aagaacacaa catctgacga
     2401 ggttaataat gggaagcccc tctctgtatt ctgcccgtaa aacaaccctg gcgttggcag
     2461 tcgccttaag tttcgcctgg caagcaccag tatttgccca cgggggcgaa gcgcatatgg
     2521 tgccaatgga taaaacgctt aaagaatttg gtgccgatgt gcagtgggac gactacgccc
     2581 agctctttac cctgattaaa gatggcgcgt acgtgaaagt gaagcctggt gcgcaaacag
     2641 caattgttaa tggtcagcct ctggcactgc aagtgccggt agtgatgaaa gacaataaag
     2701 cctgggtttc tgacaccttt attaacgatg ttttccagtc cgggctggat caaacctttc
     2761 aggtagaaaa gcgccctcac ccacttaatg cgctaactgc ggacgaaatt aaacaggccg
     2821 ttgaaattgt taaagcttcc gcggacttca aacccaatac ccgttttact gagatctccc
     2881 tgctaccgcc agataaagaa gctgtctggg cgtttgcgct ggaaaacaaa ccggttgagc
     2941 agccacgcaa agccgacgtc attatgctcg acggcaaaca tatcatcgaa gcggtggtgg
     3001 atctgcaaaa caacaaattg ctctcctggc aacccattaa agacgcccac ggaatggtgt
     3061 tgctggatga tttcgccagt gtgcagaaca ttattaacaa cagtgaagaa tttaccgctg
     3121 ccgtgaagaa gcgcggtatt actgatgcga agaaagtgat taccacgccg ctgaccgtag
     3181 gttatttcga tggtaaagat ggcctgaaac aagatgcccg gttgctcaaa gtcatcagct
     3241 atcttgatgt cggtgatggc aactactggg cacatcccat cgaaaacctg gtggcggtcg
     3301 ttgatttaga acagaaaaaa atcgttaaga ttgaagaagg tccggtagtt ccggtgccaa
     3361 tgaccgcacg tccatttgat ggccgtgacc gcgttgctcc ggcagttaaa cctatgcaaa
     3421 tcatcgagcc tgaaggtaaa aattacacca ttactggcga tatgattcac tggcgtaact
     3481 gggattttca cctcagcatg aactctcgcg tcgggccgat gatctccacc gtgacttata
     3541 acgacaatgg caccaaacgc aaagtcatgt acgaaggttc tctcggcggc atgattgtgc
     3601 cttacggtga tcctgatatt ggctggtact ttaaagcgta tctggactct ggtgactacg
     3661 gtatgggcac gctaacctcg ccaattgcac gtggcaaaga tgcaccctct aacgcagtgc
     3721 tcctgaatga aaccatcgcc gactacactg gcgtaccgat ggagatccct cgtgctatcg
     3781 cggtatttga acgttatgcc gggcctgagt ataagcatca ggaaatgggc cagcccaacg
     3841 tcagtaccga acgccgggag ttagtggtgc gctggatcag tacagtgggt aactatgact
     3901 acatttttga ctggatcttc catgaaaacg gcactattgg cattgatgcc ggtgctacgg
     3961 gcatcgaagc ggtgaaaggt gttaaagcga aaaccatgca cgatgagacg gcgaaagatg
     4021 acacgcgcta cggcacgctt atcgatcaca atatagtggg tactacacac caacatattt
     4081 ataatttccg cctcgatctg gatgtagatg gcgagaataa cagcctggtg gcgatggacc
     4141 cggtggtaaa accgaatact gccggtggcc cacgcaccag taccatgcaa gttaatcagt
     4201 acaacatcgg caatgaacag gatgccgcgc agaagtttga tccgggcacg attcgtctgt
     4261 tgagtaaccc gaacaaagag aaccgcatgg gcaatccagt ttcctatcaa attattcctt
     4321 atgcaggtgg tactcacccg gtagcaaaag gtgcccagtt cgcgccggac gagtggatct
     4381 atcatcgttt aagctttatg gacaagcagc tttgggtaac gcgttatcat cctggcgagc
     4441 gtttcccaga aggcaaatat ccgaaccgtt ctaatcatga caccggtctt ggacaataca
     4501 gtaaagataa cgagtcgctg gacaacaccg acgccgttgt ctggatgacc accggcacca
     4561 cacatgtggc ccgcgccgaa gagtggccga ttatgccgac cgaatgggta catactctgc
     4621 ttaaaccatg gaacttcttt gacgaaacgc caacgctagg ggcgctgaag aaagataagt
     4681 gattgtttca gacaaaaaaa cggcaccagg tgccgttttt tacttatgag cgaaccagat
     4741 taataccgta cacacaccga cttagtttca caccaaccgt ccagccagtc ggggccaaaa
     4801 tcacggcccg ttcctgactg cttcatccca ccaaacggta agttagcgtc aattaaggta
     4861 tggctgttta cccacaccgt ccctgcctgt aagcgatcgc tatattccag agcctgggag
     4921 agattttgcg tccagacact ggcagttaag ccatattccg tgtcgtttgc cagttgtaac
     4981 gcctcttctc catccgctac tcgcaccagg tttaccaccg gaccaaacac ctcttcacga
     5041 gttaagcgca atttagcatc gggatttacc accagcgttg gcgcaacata atacccctct
     5101 ccggctggtc cattcgaccc gcgaatcagc tctgcttgct gtgcctgcgc atcgtcgagg
     5161 aatgaacaca ctttgtcgca gtgcgcacga gaaaccaaag ggttaatctg tgcaacaggt
     5221 gacatccccg gtcccacttg caacgatttt accgcctgct caaatccact aaccagcgtg
     5281 tcaaacaacg gcgcttcaat ataaattcgc gaactggcgg cgcatacttg cccttgattc
     5341 aggaagcttc cggtcatcaa gccttcaata acccattgcg gatcagcatc ttttaataca
     5401 attgcagggt ttttaccgcc cagttccagc gttacacgcg ttaagtgatc agcagcagtt
     5461 ctggcaatac cttttcccgt cgcggttgaa ccggtaaaac tgattttcgc aacatgagga
     5521 tgtgacgtca gggccgcgcc gcatacagca cctgacccgg tgaccacatt aaaaacgcca
     5581 tcagggatac cagcctcgct ggccagttcc gccacgcgca acatcgtcag tggcgtggtt
     5641 tccgaaggct taatcacgat tgaacagcct gctgccagtg ctggcatcac cttccacata
     5701 ccaatcatca acggaaagtt ccatggcaca attcccgcca ctacgccaac cggctcttta
     5761 cgcgtccagg cctgataacg cgccccctgg ggtaagggaa tcgacaagtc cagcgtttta
     5821 cccgcgattt tggtcgttaa cccggcggta taacgcatcc agttcagcgt acagcccact
     5881 tcaaaagcac gggaaatggc aattgacttg ccttgctcca gggtttccag ttgcgccagc
     5941 tcctcactgt gctgctccac cagatcagca aaacgtagca gaatacgttc acgctctgcg
     6001 ggtaatcgcc cggcccagcg acgcgagaca aaggcccgcc aggcagacat gactgcgtta
     6061 tctacatccg cttcgttggc atcagcagta gacgcaattt cttgcccggt ggccggatca
     6121 aagatcgcca atcgtttttc actttgtgcg gggccaggac gaccatcaat ataaagaccg
     6181 tgttgacgat cgagaaactg ttggacctgg cttaatactg ctacatgcgg ctctgtcatc
     6241 acttttcctt atcatttacc cagtgtgatt tcagtgtacg cagcgacaag aaaagtcact
     6301 tttttcgctg tgacattcgc tttgtcagct gtgacaagct ccgcaaatcg tgacaataac
     6361 cgcttatgtc tctatgcctg acaagactta tttcctatag tcaggctcat tagcttcgtt
     6421 tattgcaaca caaatgcaac aataaaaata tatttcacag agcgaaaacg tgccatgaac
     6481 cccgcagtgg ataatgagtt tcagcaatgg ctttcccaaa tcaatcaggt atgcggaaat
     6541 tttaccggac gcctgctgac tgagcgttac acgggtgtac tggacacgca ttttgccaaa
     6601 ggactaaagc tgagcaccgt gacaaccaac ggagtgaatt tataccgcac ctggcaggag
     6661 gtaaaaggca gtgacgatgc ctggttttac accgtttttc agcttagtgg tcaggcgata
     6721 atggagcagg atgagcgtca ggtgcagatt agcgcaggcg atattacgtt actcgatgcc
     6781 tcacgcccct gttcgcttta ctggcaggag tcttctaaac agatttcatt acttttgcca
     6841 cgcactctgc tggaacaata ttttccccat caaaaacctg tctgcgcaga aagactggac
     6901 gctgacttac ccatggtgca actcagtcat cgcctgttac aggagagcat gaataatccg
     6961 gcactttctg aaacagaaag tgaagctgcg ctacaggcga tggtgtgtct gcttcgccca
     7021 gtacttcatc agcgggaatc tgtccaacct cgccgtgaac gtcagtttca aaaagtggtt
     7081 acgttgatag acgataatat tcgcgaagag atattacgcc cggagtggat agccggagag
     7141 acaggtatgt cagtacgtag tttgtaccga atgtttgccg ataaaggttt ggtagtcgcg
     7201 caatatattc gtaaccgtcg tctcgatttt tgtgcagatg cgattcgcca tgccgcagat
     7261 gatgaaaaac tggcaggcat tggctttcat tggggatttt ccgaccagag tcatttttca
     7321 acggtattta agcaacgctt tgggatgacg cctggcgagt atcgccgtaa attccgctaa
     7381 ctaattggcg ttgcatgtac tgcaacgcca attagttaaa acagatattt gactatgcat
     7441 ttttcagggc aaaaggcaat ttgacaggag tttttgaata ttcaccttgt ttcaggaaaa
     7501 tagatcaatc gctttctcct gtagcgttat cccgatagtg cggttttgct attaaaattt
     7561 tcgcacccat ttaccgttta tcccttgtac atattccccc ggtttagccc gagctacgag
     7621 cttttgcccg gcgagtttcg cgatatcatc taccgacaca ttattttgct ttgccagttg
     7681 ttggtaactc gctttacgtt cggcattaat gtcggttact aatttctctg tttcagcatc
     7741 cgttttcagc gcaaccagat aaccataaaa tgtttccccg acccgcccct gagttctggc
     7801 ttcatccaga gtcaatgcca ttacattgct gcttaccagc ccaacaagaa acgcacaaag
     7861 aagtaatgtt ttcttcatca tcgcctcaga aaagatcgct acgagtttca agcagctctt
     7921 cgacatcttt gtctgccttg atgatgatct catgctcaat tttaacgttc atattgatag
     7981 tgatcggttc cttaggtgca gcgacttcaa tgcgaggcgt acagccaacc agcataaaag
     8041 atgacgtcaa cgcagccagt aaaattttca ttgtttttcc tcacactctt ttccttgcgg
     8101 acagtcattt cccggcagac gtgcgttctg ctccaaccat gcctggagat tatcgccaaa
     8161 gcgtaaactg cgccacagcg taaaaatatt ctcttcatga tggtaattaa ggtttaccgt
     8221 accgctttta ccatcaacgc gactggtacc tgtaatgttg gcctgcatgg ttaataaacc
     8281 gagattatct aaattaatat ttgtcgatga acggctaatt tccatatagc gcaaccagtt
     8341 aattgctgaa cccgcagtca tattgtcttt caccaccgca tctgcggtgt ctttatccag
     8401 ccgcaaggtc attggcccgc tatttgccag ccaaccatct ttcactatcc atttttcgtt
     8461 gtttagccat aacggcaatg caccactaaa tgccccggac atggcgaatt gtttcggatt
     8521 gacggcgcta accagttcgc tggatgagag attattcagc cgcaacagcg ccgggtcatg
     8581 ttgcggcata cgtaattgtt tcatcagcac gttaccgcct aacacatcga cgctaacatc
     8641 agtcaacagc aagggttctt cttcagtcca cggataacgc ccttgcaaat cagccgtaat
     8701 atttttcgct gtcaccagat taatcacttc ggcaattcgc aacgtaacgg ggccgcgagt
     8761 ccccagatgc caggctccat cggcaaaacg gaaaggcagg acaaaatcga caccgttcac
     8821 ctgattatct ggcatccagg cactaccgcc tttcaacacg ccgtgccctc ccgcgcggaa
     8881 tccttgttca ggcgcagcag aaaatgcaac ctgagcatac agttcaccat cgcgtaagtt
     8941 catcttccag tcgggtggca ccagcggctg gaatacagtc agtgattgtt taggccacca
     9001 ggcattaccg cgcagacgaa taccgtccca gcgaccattt acccgaaccg ggccaatctc
     9061 accagcatgt aaatcgcctt taaagaggaa atacgtcgga tcgcgcccat cgacgctaaa
     9121 ttttaaggtt gatggcggta acacactgcc gccagtgaac agcgtttgcc cggcgtccag
     9181 tgacagcgcg ccgctgaagg agggatgctg tgcgtcacgt acccagacga cgggcttgtc
     9241 gagaattaat cgcggctttt ctaccgtcat cgtaccgtat tgtaactggt cgaaaccggt
     9301 ggagagatcg gtcagcgtaa tcgtgctgtc atgccactca ccttttcctg cgacatccca
     9361 ggtggcattc atcggtgtaa aactcccttt tccccagtag cgccactgcc agcggccagc
     9421 gtcagggaga aaatcattcg ccagcccatc catatgcagc acgaaatcgc ccagttcatt
     9481 ttcatgcgcc tgcaaaatgg cctgcaaacg tccgtcaaca ccacgttggg tgacttttac
     9541 acccgctaaa ggccagcgga tttcatcgat gtccagcgaa tcgatgactc ttccctttga
     9601 acgaagtaac gcgcctggct caaaggtcag cgttgggtca gacagacttc cacttagctg
     9661 cgcaggtaaa cgggcatata aaatgaggtt cgcctgtttc gcttcaccgg tcagctgcag
     9721 aggcagctgg ctgttatcca tgcttaattt tcctgggcca aaattaagca ccgcgttgcc
     9781 cttacccgct tgcccctggg tcagcacact cagtcgtccg ctgaccagag cgttctcaag
     9841 ccctgcctgc cagttgtcga ctttgacacc cagtcggcca ctcaaaggaa aacctgcata
     9901 cggccagctc cagcgaccat cgcttacggt caattgttga cgagtaattt gccacggcaa
     9961 atcgagcaac ggatcgccgt tatcccgtgc cagcacgatc aattgcccgc tattttcctg
    10021 ccagtccagc tctgcatcca ccagtgacgg ttcctgcggc aagtttaacg tcgcagtagc
    10081 atgcccactt acaggaagtc catccggcac tagcggcata gtaaattccc ccaccagttt
    10141 aaccggcggc tgattttcaa acgcgacgac atccagttcg ctgactgtaa gttgttgccc
    10201 tttcagctgg ccttgaaatt taactttttc gccctgataa cgcagttgct ggatatcgga
    10261 ggttaatgcg agagagagtt ttccctgcca ttcctgccag ggagaaaaaa tcagtttatc
    10321 gatattgatc caggtgttag gcagcatgga ctgccactgc gcgagggttt ttggagcagc
    10381 tggcgattgc tccgtctgcg gcaatttcgc cagacaagca gaatcaagtt ctaccgtgcc
    10441 gacgttcaat aaccagcggc tgggatgtga aaggctggcg ttggtgatat gcgcaagctg
    10501 acaatctccc accagataac ggagatcggg aatgattaaa cctttacgcg taatgcgtgg
    10561 gctttcatct aatgcaatac gtgtcccgag cggtagccag atgcccgcca gcgtgggaac
    10621 ccacagcccg agcgtcatca gcagcgtcaa cggcacaaga ataatcagta ataacagcgc
    10681 gagaacggct ttatatttac ccagcatggg tagttaatat cctgatttag cgaaaaatta
    10741 agcattcaat acgggtattg tggcatgttt aaccgttcag ttgaaggttg cgcctacact
    10801 aagcatagtt gttgatgaat ttttcaatat cgccatagct ttcaattaaa tttgaaattt
    10861 t
//
LOCUS       DAPGOF010000169        10828 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.170, whole genome shotgun sequence.
ACCESSION   DAPGOF010000169 DAPGOF010000000
VERSION     DAPGOF010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10828
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.170"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..850)
                     /locus_tag="RHV13_003843"
     CDS             complement(<1..850)
                     /locus_tag="RHV13_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HDV1243321.1"
                     /translation="MNDVTVVTSVTYPSPESLALVADVQYHEPYLSAALNRKFRGIVD
                     PGFYAGFLPKPGGGMNLLITSVDGDKTAGAASVDIGEFYQVTIQHRKDISLALSAGKK
                     YAIVLKGRYLLGEDTYQVNTASHIHAAEFVARTYTDSYQLGDGELLVCTVNIPAGVSA
                     ITQEMIDTSERINRTIGIDISDSVTSSRSDVAASSLAVKKAYDLAKSKYTAQDASTTQ
                     KGLVQLSSATNSDSETMAATPKAVKSIKDLADTKAPIESPSLTGTPTAPTAAQGTNST
                     QIANTAF"
     gene            complement(862..1296)
                     /locus_tag="RHV13_003844"
     CDS             complement(862..1296)
                     /locus_tag="RHV13_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV1243322.1"
                     /translation="MSDVSTNLYKSQLLDYYYQRRAESSINKGSRFLISKAVFGTSSL
                     VTKKGDGTYEIGELPKVFDLAELTSQFCTINLVPTYSGGIITVRMDLDQSQLQEGKNY
                     PFNTLVVLDNENKPIAIICVQEDSLYVGKTYTAVMAINTTTA"
     gene            complement(1375..2211)
                     /locus_tag="RHV13_003845"
     CDS             complement(1375..2211)
                     /locus_tag="RHV13_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV1243323.1"
                     /translation="MQRSWFNNRLTSAKQKSLLYKSLADLVQSMMDTFVDPWLERITN
                     RKSIFSMSKEDLETRTNELGQFFTIRTSNSSSVPMLLQQRLDEIHFKGTERPINQTIY
                     REFNGISVLWDPIYAPVDLERHPYGTVLIPESTLETTGGTFGEMFLTSRGMISIPIND
                     LARTMGITGTIDQSAITEEILRKFNQFVKPLLPLHIVFDGLTLYLSVVVNEQADMITL
                     NEISDTEKAFCWFETSDTTSLTGVTSISAPITATPGGTIVKATPTFDRTRADDLLLDS
                     DA"
     gene            complement(2211..3644)
                     /locus_tag="RHV13_003846"
     CDS             complement(2211..3644)
                     /locus_tag="RHV13_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin hydrolase"
                     /protein_id="HDV1243324.1"
                     /translation="MSKTTPTKDSIRAEFEELVEKDSFWSKFVGSQFVSMLTLFITQI
                     VYRCFQYADAALAEGFISTATRRSSILAAAETNSYVGTKPTPSSGMIEITATSEDAPA
                     VIPKNMPLISDDQYPYMTMDVCRLVDGTGTVEVAQLEIQEVTYTVTAAKEFLEVVLSK
                     ALTAVCYKLEVFVTTDGKTTQWSSSTMFRLAGSKSQVYVEFYKPSEQLGVRFGDGLIG
                     QIPPEGSTITLKVWCTNGDITLVAGQNLTPVDSAANLANLISVKTTTPITAGTDAETT
                     EITRNRAQYYLAYDDQVVWGGDYTYFLVRNIPGLSWVKAWGEGQQEKLDGAYNVQNIN
                     KIFISGWHPNKSQSELEEMILAAFKKVPNELNKKFSYKEVRKLPFKITITGRISASLT
                     IENVTDELKSALETKFGRDSTFFDPNRVGKYILIKKKDVWAFIETLGYFRDFYLEFVE
                     WNESNGFYDFVYLDTENSTFNISYEEE"
     gene            complement(3641..3997)
                     /locus_tag="RHV13_003847"
     CDS             complement(3641..3997)
                     /locus_tag="RHV13_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243325.1"
                     /translation="MANNNEIDPLLTLELSGVKTYESQEEAWGARLYEWLNTYQGEVY
                     GDPSWGNVLPQFKHEPTNLSHVQIAVEAMLLQKLTVDLPDIPISGLSVAEGDAFDKLK
                     ISIRIRDITITQDVVL"
     gene            complement(3997..7419)
                     /locus_tag="RHV13_003848"
     CDS             complement(3997..7419)
                     /locus_tag="RHV13_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HDV1243326.1"
                     /translation="MERKNANIDDVIRTVETASAKELEELAGIREAVEDLKGGRVATV
                     DPVSRSVSALNRTIENSRPDFVANAPSVDPIVEAMKRLNLGDVSRVVQEDVALQEPQA
                     KSTTRKGKKRRKKAITEDVKAQRTEAAEHAREMFGQKGGAQKSQNQRDARGRFIGKSG
                     SKAAAEDARAERAEKARRKEDDERLNAESGLLKKLSKVAEGIGNPSETRAVDALGYAV
                     AGPLWAAGKELGGISKEVGGSLNGARKSIADVIRGNDDNSRRKGFFRRKSQNSADVVQ
                     VNTQKRTVQELQDQTSEIKEGNDKILSALDQIAKNTGKKKGGLLSKLFSLLGKGAGGV
                     ASLLMGRGMLKKAGALAFGALGAKKLVGMLRGGGKKTLAHEGGDLAARAAGKLGLKAV
                     GKGALRAIPLVGTVAGGIYDAVTGWNDTEAQRRAFGLKSGQDPSFQQKAAYTLANVLD
                     LGGLVSGISSAIGDVLKSLGFEDIGNMLQSFSTESIAQAIDSGITNLETYISNLGDTI
                     STKFDDYTAKIGDAVSAWFSDTSNKLLEKLDAIKDFFTVDNLKQVFSDAIDSAIDFIK
                     NPGKHIKEAAGNIWDGVKNLPGKALDAAVDAVKNTPAAMIVSKIPNPIGEANAKEITP
                     ELKAPVNSHQETSNSKTESDAKQSNIATRVINAALDTAKDSNKTVKQTANQIINANAV
                     ETGNSALQKIDKAIGQNSSSSSSLNTTGTRNDIQKAADTYNNGNLDVKVGSLGAEGKA
                     NLDKLAPYFAELENKYGLPEGTLYAIAATESGGDPNAKSTLTRSPNGKLSGGALGMFQ
                     FTSVAREETGLSREDSFNPEKSAEAAALLMSKYLKQANGDLNEAITAYNAGFGTINKW
                     KKGTGDLSKENREYAIKVNTHRARYLGGEIYTPGAGAQGGAQYGVRGPLPDNAVIDQS
                     TGLAFTPGDSPFEKGGLVDKIGNAVGVNDLVNKFMNGRGMRREVVQGTLEERARGKGT
                     ATAAGNVYVDTPMPVEEARPVASNSSYFDQLGAQMGIDGLFDKLRNSPGMRKNNAPEP
                     ASTSLVTTAANDLQQPTGRMQIDGQVISDLGGSGAKPTMQLADNTVSLDGETKRLFAQ
                     MTSLLARIEEHTKDSAKGQGTVVKVSTPQPGVMRTVPLSIDDPLMNDYARVD"
     gene            complement(7501..8382)
                     /locus_tag="RHV13_003849"
     CDS             complement(7501..8382)
                     /locus_tag="RHV13_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="morphogenetic protein"
                     /protein_id="HDV1243327.1"
                     /translation="MLLPLFPLPSRPTELIQFRQPNIADAMRFNSITPEEQEQQTTAY
                     LKALLAEPAKHDPLTWTAQDRITALWWIFTGSRETPVETFTYTCKHCGKEHYYDCDMN
                     ALAEDIQVLEVEPFIDDIEVSVEGVPYQWRIVPLDGWAMEMLEMRRAALPPEDDAEFK
                     EAIVDLRFWEFAYQCELYNDVSGTREEQAERRYETIKRMAIDTEFMKLAAHIRLAHEK
                     LEHGLPCYIDKGEMRLRLPPHKCPNQDTKESTEGAYTRLWVPFRATDFIPQVGIEKLS
                     DLSVQPGFVWGYTDSGR"
     gene            complement(8397..9008)
                     /locus_tag="RHV13_003850"
     CDS             complement(8397..9008)
                     /locus_tag="RHV13_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV1243328.1"
                     /translation="MGLNVASVKSYVSSALTTTLFGSGVGEREVGKLTSIIMNKMLFA
                     QGWQFSVEVDGLEGADFFAKDITYHDYSIEYETIKIGGGNILQPTERSPGQITMMVRD
                     TVDGLVLDWFKTAKSRVINPDGTGNIPSKYLLNVRIYRLLSSGLTKLENEMTVFPVTT
                     GDVTYARDQVTEFKSFPMTFALHSTFNQSSSSLASLLGFSFSL"
     gene            complement(9019..9585)
                     /locus_tag="RHV13_003851"
     CDS             complement(9019..9585)
                     /locus_tag="RHV13_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243329.1"
                     /translation="MTPRQLLEDVKSRFTPLIADEPALLESLLRKALGTYQDRAGHIK
                     RIRFTDQTCKSLACPADFLALVSVTDHTGDLVYSDVYDGNIELEDTHRAVYPLNVSYL
                     ANLRDMDLDNGEVPPEIIGLLSDYLEVLIAIPNTDRLRRISIAGKLDASNLSDENTLY
                     QRKLDLEEKMSATRAIIPGIVLFSSMLK"
     gene            complement(9666..10205)
                     /locus_tag="RHV13_003852"
     CDS             complement(9666..10205)
                     /locus_tag="RHV13_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HDV1243330.1"
                     /translation="MNKIILFLIFSTFSVGTALANSLQSQIAAIAQAENEGRAKEQQA
                     EDARKELIRQQAQAERIRREKAASAAAAREKQRVAAENERRAKREAELANDKKRDQAY
                     EDELRKLQLESMKLELQAKAARVQRENDFIEQELKERAAKTDVIQSEADANRNISTGS
                     KDLLQSEGKAREKKASSWW"
     gene            complement(10209..10721)
                     /locus_tag="RHV13_003853"
     CDS             complement(10209..10721)
                     /locus_tag="RHV13_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243331.1"
                     /translation="MSKKYTLCALVVSAILLSGCQSSGADYAADVYDTAQLNSKQETK
                     TVNIISVLPAKVKVDNKANKEAAQTFGAVLGAVAGGVAGYNVKGTSTLGAVAGGTGGA
                     ALGAAAGSLVSDKTIVEGVSLTYKEGTKVFTSTQVGKACQFTTGLAVLISTKDNETRI
                     QPNATCPEKK"
BASE COUNT         2688 a         2768 c         2301 g         3071 t
ORIGIN      
        1 caaaggctgt atttgcgatc tgcgtgctgt ttgtaccttg cgctgccgtc ggcgcggttg
       61 gcgttcctgt cagactcggg ctttctattg gcgctttggt atcagccaga tcttttatag
      121 acttaacggc tttaggggta gccgccattg tttcgctgtc gctgttagtt gcgctactga
      181 gctgaactaa tcccttttgc gttgtgcttg catcctgcgc cgtatacttg cttttcgcca
      241 gatcgtaggc ttttttaact gccagcgaac ttgcagcaac atcacttctg ctactggtta
      301 cagagtctga aatatcaatg ccgatcgtgc ggttaatacg ctcggatgta tcaatcatct
      361 cctgggtaat ggctgatacg ccagcaggga tattcaccgt acaaacaagc agctccccat
      421 ctcctaactg atatgaatcg gtataggttc gggcaacaaa ttcagccgca tgaatatgtg
      481 acgcggtatt aacctgatag gtatcctctc caaggaggta tcttcccttc agcacaattg
      541 catatttctt gcctgcacta agtgcaagag aaatatcctt acggtgctga atagttacct
      601 ggtagaattc accaatatcc accgacgccg cgcctgcggt tttatcacca tccactgagg
      661 tgattaacag gttcatccca ccgccaggct taggtaagaa accggcataa aatcccgggt
      721 caacaatccc cctgaatttt cggtttagcg cggctgacag atatggttcg tggtattgca
      781 catcagccac cagagccaac gactcgggtg atgggtaagt aactgatgta acaactgtaa
      841 cgtcattcat caagcatatc cttatgctgt tgtcgtgttt atggccataa ctgcggtata
      901 tgttttgccc acatacagcg agtcttcctg gacacaaata atggcgattg gcttgttctc
      961 gttatccaga acaaccagag tgttgaatgg gtagtttttc ccttcctgca actggctttg
     1021 atcaaggtcc attcggacag taattatccc gcctgagtag gttgggacga ggttgatggt
     1081 gcaaaattga ctggtcagtt ctgccagatc gaaaaccttt ggcagttctc caatctcata
     1141 agtgccatct cctttcttag taaccagtga actggtaccg aaaacggcct tgctgattaa
     1201 aaatcgagag cctttgttaa tggacgattc agcgcgccgc tgatagtaat agtccaacaa
     1261 ctgactctta tagaggtttg ttgagacgtc agacatgatt ttccctaatc aatgttgtga
     1321 agcctcattg taagagaagt aacttgtcac cccgccctgc ggacggggtg attgtcaggc
     1381 gtcgctatcc agcaacaaat catctgcgcg tgtgcgatca aacgtaggtg tcgctttcac
     1441 aatagtgcca ccaggcgttg cggtgatcgg ggcgctaatc gacgtaactc cagtaagcga
     1501 agttgtatcc gaagtttcaa accagcagaa tgctttttcg gtatcagaaa tctcgttcaa
     1561 agtgatcatg tcggcctgtt catttacaac aaccgacaaa tagagcgtaa gcccatcaaa
     1621 cactatatgc agtggcagta gaggctttac gaactgatta aactttctga gaatttcttc
     1681 tgtaattgcg gactgatcta tcgtgccagt aatacccatt gtcctggcca ggtcgtttat
     1741 gggaatactg atcatccctc tggaagtcag aaacatctcg ccgaatgtgc cgccggtagt
     1801 ctccagtgtg ctttctggta ttagaaccgt gccataggga tgacgctcaa ggtccaccgg
     1861 tgcatatatc ggatcccata aaacagaaat accgttaaat tcgcggtaaa ttgtctggtt
     1921 tatagggcgt tcagtcccct taaagtgaat ctcatcaaga cgctgttgta acaacatcgg
     1981 aacggaagat gagttcgacg ttctgatagt aaagaactgg ccaagttcat ttgtcctggt
     2041 ctccagatcc tccttgctca tggaaaaaat agacttccgg ttggtaattc gctccaacca
     2101 tgggtcaaca aaggtatcca tcattgactg aaccaaatca gccaatgatt tatagagcaa
     2161 tgacttttgc ttagctgatg taagccggtt attaaaccag gaacgctgca tcactcctcc
     2221 tcatacgaaa tattaaaggt ggagttttct gtatccagat aaacgaaatc gtaaaagccg
     2281 ttggactcat tccactcgac aaattccaga taaaagtcgc ggaaataacc cagcgtttcg
     2341 ataaatgccc aaacgtcttt tttcttgatt aggatgtact tgccgacacg gttcggatca
     2401 aagaaagttg agtcacgccc aaattttgtt tccagtgccg acttcagctc atcagtcacg
     2461 ttctcaatgg tcaggcttgc cgatatccgc ccggtgatgg tgatcttaaa gggtagtttt
     2521 ctgacctctt tatacgagaa tttcttgttc aactcattcg gcaccttctt aaaggcagcc
     2581 aagatcattt cttcaagctc tgactggctt ttgtttggat gccatcctga aataaatatc
     2641 ttattgatat tctgaacatt ataagcacca tctaattttt cttgctgacc ctcgccccat
     2701 gcctttaccc aggacagtcc cgggatgtta cgcaccagaa aatacgtata gtccccgccc
     2761 catacgacct gatcatcata ggcaaggtaa tattgtgcac ggttacgtgt gatctccgtt
     2821 gtttcggcat cggtacctgc ggttataggt gtcgttgtct taactgaaat caaattagct
     2881 aaattagccg cagaatcgac aggcgtcagg ttttggccag caaccagggt tatatcgccg
     2941 ttggtgcacc ataccttaag cgtaattgtc gagccttctg gcggtatttg cccaattagc
     3001 ccatcgccga atcgaacacc caactgctcg gatggtttat aaaactcaac gtagacctgg
     3061 cttttactac cggctaaccg gaacatagtg ctggaagacc actgcgtggt cttaccatcg
     3121 gtcgtcacga atacttccag cttatagcag acagcagtga gagcctttga taacacgact
     3181 tccagaaatt ctttggctgc cgtaacggta tatgtcacct cctggatttc caactgtgcc
     3241 acttctaccg taccggtgcc gtcaaccaac ctgcatacat ccatagtcat gtaagggtac
     3301 tggtcgtcag atattaaagg catgtttttg gggattaccg ctggggcatc ttcacttgtg
     3361 gcggtgatct caatcatccc cgatgacggt gttggcttgg taccaacgta actattcgtt
     3421 tctgccgctg ccaggataga ggaacgccgc gtcgcggtcg atataaagcc ttcagccagc
     3481 gccgcatcgg catactgaaa gcacctgtag acaatctggg taataaacaa tgtcagcatc
     3541 gagacaaatt gagagccgac aaacttcgac cagaatgaat ctttctcgac aagctcttca
     3601 aactctgcac gaatactgtc tttagtcggt gttgttttac tcatagcacc acgtcctgtg
     3661 tgatagttat atccctgata cgaatggata ttttcaactt atcaaaagca tctccctcgg
     3721 ctactgacaa gccagaaatc ggtatgtcag gtaaatctac cgtcagtttt tgcaacagca
     3781 ttgcctcaac cgcaatttga acatgcgaca agttggtcgg ttcgtgttta aactgcggta
     3841 aaacattgcc ccatgacgga tctccgtata cctcaccctg ataagtgttt agccactcat
     3901 ataaacgagc gccccaggcc tcctcctgag actcatacgt ttttacgccg gataactcca
     3961 gcgtcagtaa aggatcaatt tcattattgt tggccatcaa tcaactctcg cgtagtcatt
     4021 catcaacgga tcatcaattg acagtggcac cgtgcgcata acgcccggtt gaggcgtgct
     4081 gacctttacg acagttccct ggcctttcgc cgagtcttta gtgtgctctt caatcctggc
     4141 aagcaatgag gtcatctgcg caaacagccg cttcgtttca ccatcaagtg aaacggtatt
     4201 atcagccaac tgcattgtcg gcttggcacc ggagccgcca aggtcactaa taacctgtcc
     4261 gtctatctgc atacgaccgg ttggttgctg caaatcgttg gcggcagtcg tcaccaggga
     4321 cgtggaggct ggttcaggcg cattattttt ccgcatcccc ggcgagttgc ggagtttatc
     4381 gaatagtcca tcaatcccca tttgtgcgcc gagttggtca aagtaacttg agttgctggc
     4441 caccggacgc gcctcttcaa ctggcatcgg agtatcaaca tacacattgc cagctgctgt
     4501 tgcggtcccc ttccctcgtg cacgttcttc gagcgttccc tgaacgactt cccgacgcat
     4561 accccggcca ttcatgaatt tgttgaccag atcgttaacg ccaacagcat tgccgatttt
     4621 gtctaccaga ccgcctttct caaacgggct atcaccaggg gtaaacgcca agccagtaga
     4681 ctgatcgata acagcgttat caggcagtgg tcccctcact ccatattgcg ccccaccctg
     4741 tgctcctgct ccaggtgtat agatttcacc acctaaatag cgagcacgat gagtattgac
     4801 cttgatcgcg tactcacggt tttctttcga taagtcacct gtgccttttt tccacttatt
     4861 gatagtgcca aacccagcgt tatatgcagt gatggcctcg tttaagtctc cattggcttg
     4921 cttcagatac ttgctcatga gaagagccgc agcttctgcc gatttttccg gattaaaaga
     4981 atcttcccgg gataatccag tttcctcacg agcaacgctc gtaaactgga acattccgag
     5041 agcgccacca cttagctttc catttggtga tcttgtaagc gtagattttg cgttaggatc
     5101 accaccagat tcagttgcag cgatcgcgta aagagtacct tcaggaaggc catatttatt
     5161 ctctagttca gcaaaatacg gagccaactt atcgagattt gccttacctt cagcgccaag
     5221 acttccgact tttacatcca agttgccatt attgtaggta tccgcagctt tctgaatgtc
     5281 attcctggtg ccagtggtat taagcgagga tgatgacgag ctattttgac caatagcttt
     5341 atcaattttc tgcaacgcgc tattgcccgt ttctacggca tttgcattga taatctgatt
     5401 ggcagtttgt ttaactgttt tattgctatc tttcgctgtg tccagtgccg catttatcac
     5461 gcgggtagca atattactct gtttggcatc ggattcagtt ttagaattag acgtctcctg
     5521 gtggctatta accggagctt ttaactctgg agtgatttct ttcgcattag cctcgccgat
     5581 cggattgggt atttttgata caatcattgc cgcaggggta tttttaacgg catcaaccgc
     5641 tgcatctaat gctttaccgg gtaaattttt aaccccatcc caaatattac cagccgcctc
     5701 tttaatgtgt ttccctgggt tcttaatgaa gtcaattgca ctatcaattg catcactgaa
     5761 aacctgtttc aggttatcga cagtaaagaa gtctttgatg gcatccagct tttcaagcag
     5821 cttattagat gtatcgctaa accatgctga aacagcatca ccaatctttg ctgtgtaatc
     5881 atcgaacttg gtagaaatgg tgtcgccaag gttagaaata tatgtttcta agttggtaat
     5941 cccactatca atggcctggg caatactttc cgtcgaaaat gattgcaaca tattgccgat
     6001 atcctcaaat ccaagtgatt tgagaacatc accaatagca ctgctaatac cagataccag
     6061 tccccccaaa tcaagaacat tggctaacgt ataagcggct ttttgctgga atgatggatc
     6121 ttgtcctgat ttaagcccaa acgctcgacg ttgcgcttct gtatcattcc aaccggttac
     6181 cgcatcataa atacctccag ccactgtgcc gactagggga attgcgcgta acgccccttt
     6241 accaactgcc tttaatccaa gtttacctgc tgcccgggca gctaaatctc cgccttcatg
     6301 ggcgagagtc ttcttgccac caccgcgtag cattcctaca agtttctttg cccccagagc
     6361 gccaaaagcg agtgctccag cttttttcag catgccacgc cccattaaca acgacgcgac
     6421 gccaccggcc cccttcccta acaggctaaa taatttggac agcaagccgc ccttcttttt
     6481 cccggtgttt ttggctatct gatcaagggc gctgagaatc ttgtcattgc cctctttaat
     6541 ttcgctggtc tgatcctgaa gttcctgaac cgtccgtttt tgggtgttaa cctgaacgac
     6601 atcggcacta ttttgcgatt tacgcctaaa aaaacctttt ctacggctgt tatcgtcatt
     6661 gccacgaatc acatcggcaa tagactttct ggcaccatta agcgatccac caacttcttt
     6721 tgatatcccg ccaagctcct tccctgctgc ccacaatgga ccagcaacgg cataacctaa
     6781 cgcatcgacg gcacgagtct ctgaagggtt acctatgcct tcagctactt ttgacagttt
     6841 ttttaataaa cctgattcag catttagacg ctcatcatcc tctttgcgcc tggccttttc
     6901 agcacgttca gcacgggcat cttccgctgc ggccttactc cctgactttc caataaaacg
     6961 accacgcgca tcgcgttggt tttggctttt ttgcgcaccg cctttttgac cgaacatttc
     7021 gcgagcgtgt tcggctgctt cggtccgttg cgcctttaca tcttctgtta tagccttctt
     7081 gcgtcgtttt ttaccctttc gcgtagttga tttggcctgc ggttcctgta gagcaacatc
     7141 ctcctgaact acacgagaaa cgtcccctaa attaagccgt ttcattgcct caacaatagg
     7201 gtccactgat ggcgcattgg ccacaaagtc tggccgggaa ttttcgattg tgcgatttaa
     7261 tgccgacaca ctgcgagaga caggatcaac tgtagcaact cgcccacctt tcaaatcttc
     7321 aacggcttcc cgaatacctg caagctcttc cagctctttt gcgctggcgg tttcaaccgt
     7381 ccttatcaca tcgtcaatgt tggcgttttt tctttccatg atcttatcgc ctaccgcttc
     7441 ggtttaagtt tttcttccag tttctccaac aggaaaaacg cataggattc agtgagcctt
     7501 tcagcgtcct gaatcggtat acccccatac aaaaccaggt tggacactaa ggtctgatag
     7561 cttttcaatc cccacctgtg gaatgaagtc ggtagcccga aagggcaccc acagacgggt
     7621 atacgcaccc tctgtggact cctttgtatc ctggtttggg catttgtgcg gcgggagacg
     7681 aagacgcatt tcgcctttat cgatgtagca cggtaaacca tgttcgagct tttcatgagc
     7741 cagccggatg tgtgccgcca gcttcataaa ttcagtatca atggccatcc gtttgatcgt
     7801 ttcataacga cgctcagcct gctcttcacg agtaccgcta acatcgttat aaagttcaca
     7861 ctgataagcg aattcccaaa aacgcaaatc aacgatcgct tctttgaatt ccgcgtcgtc
     7921 ttcaggtggc aatgctgcac ggcgcatctc cagcatttcc attgcccaac catcaagcgg
     7981 cacgatacgc cattgataag gcacgccctc tacagacacc tcaatatcgt caatgaacgg
     8041 ttccacttcc aggacctgga tatcttcagc cagagcattc atatcgcaat cgtaataatg
     8101 ctctttaccg caatgtttac aggtgtaggt gaatgtctcg accggtgttt cacgggagcc
     8161 ggtaaatatc caccataacg cggtaatccg gtcctgcgcc gtccatgtca ggggatcatg
     8221 tttcgcgggt tcagccagca aggcttttaa atacgccgtt gtctgttgtt cttgttcctc
     8281 cggtgttatc gagttgaaac gcatcgcatc agcaatattt ggctgacgga actggatcaa
     8341 ttcagttggc cgcgatggta gcgggaaaag gggtaaaagc atccttgctc cttaattcaa
     8401 agagaaaaac taaagcccag aagggaagcc aaagaacttg aggattggtt aaacgtgctg
     8461 tgcaatgcga aggtcattgg gaatgactta aattccgtaa cctgatcccg cgcataggtg
     8521 acatcgccgg tagtgaccgg gaataccgtc atctcatttt ccagtttggt taagccggaa
     8581 gacagcaacc gataaatacg cacattgagc aaatatttag acggtatatt cccggtaccg
     8641 tccggattga tcacccgact ttttgccgtc ttaaaccagt ctaaaacgag gccatcaacg
     8701 gtatccctga ccatcattgt tatctgccca ggcgaacgct ccgttggttg aaggatattc
     8761 cctccgccga ttttaatcgt ttcatattcg atgctgtaat cgtggtaggt aatgtctttg
     8821 gcaaagaagt ctgccccctc cagtccatca acttcgacag agaactgcca tccttgcgcg
     8881 aacagcattt tgttcatgat gattgacgtc agcttaccaa cttcccgctc accaacgccg
     8941 gagccaaata atgtcgtcgt taatgccgaa gatacataag actttactga agcaacatta
     9001 agccccatat cagccccctc acttcaacat ggatgagaaa agaacaatcc ccgggataat
     9061 tgcccttgtt gcgctcattt tctcttccag atccagcttt cgctgataca gcgtgttctc
     9121 gtcggataaa ttgctggcat cgagtttccc cgcgatagat attcttcgca ggcgatcagt
     9181 gttaggtatc gcgattaaca cttccagata gtcagaaagt aacccaatga tttcaggtgg
     9241 cacttcccca ttatccagat ccatatcacg taaattagcc agatatgaca cattcagcgg
     9301 gtataccgct cgatgagtat cttcaagctc gatattccca tcgtaaacat cggagtagac
     9361 aagatcgccg gtatgatccg taaccgatac gagcgcaaga aaatcagcag ggcaagcaag
     9421 tgatttacag gtctgatcag tgaagcgtat ccgcttgatg tgtcccgccc tatcctggta
     9481 ggttcccaat gcttttctta gcagggattc cagtaaggca ggttcatccg caatcaaagg
     9541 tgtgaagcgg gatttgacgt cttcgagtaa ttgtcgtggt gtcattgaaa cctcgtagaa
     9601 tctagtttgt taacagattc tacgagttgt catttgtgac agtatgctcg caatcgtagt
     9661 gattactacc accaactgct agctttcttc tctctagctt ttccttcgct ttgcagtaaa
     9721 tctttacttc ctgtagaaat atttctattt gcatcagcct cagactgaat tacatctgtc
     9781 ttagctgctc tctccttcaa ctcctgctct ataaaatcgt tttctcgctg gacacgagcc
     9841 gcttttgctt gcagttcgag tttcatgctt tcgagttgca gcttgcgaag ctcatcctca
     9901 taagcttgat ctcgcttctt gtcatttgct agctcggctt ctcgtttcgc tctacgttca
     9961 ttttctgcag caacacgctg tttttcgcgt gcagcagcgg cagatgctgc tttttctctt
    10021 ctaatacgtt cagcttgtgc ttgttggcga ataagttctt ttctggcatc ctcagcttgc
    10081 tgctctttag ctcgcccttc attttccgct tgagcaatag cagcaatttg actttgcaac
    10141 gagttagcca aggcagtgcc tacactaaac gtagaaaaga ttagaaataa aataatttta
    10201 ttcataaatt atttcttttc tggacaagta gcatttggct gaattcgagt ttcattatct
    10261 ttagttgaaa taagcacagc aagccccgtt gtaaactggc atgctttacc aacttgagtt
    10321 gaagtgaaca ccttagttcc ttctttataa gtcagtgaaa caccttcaac aattgtttta
    10381 tcactaacca aagaaccagc cgctgcacca agagccgcac caccagtacc gccagctaca
    10441 gcacccagtg tcgatgtgcc tttaacatta taaccggcaa cgcccccagc aacagctccc
    10501 agtaccgcac caaatgtctg tgccgcttct ttattagctt tattatcaac tttcacttta
    10561 gctggaagca cagagataat attgactgtc tttgtttcct gcttcgaatt caattgagcg
    10621 gtatcataaa catcggcagc ataatcagca ccgctagatt gacacccaga aagaagaatt
    10681 gcagatacaa caagagcgca taaagtatac tttttagaca taagaatcat cccagaaaaa
    10741 caaaaaccgg cgagaagtta ccacgagaac ggaaagtaaa caagaatact cccattgact
    10801 tatatcaatt aatcaacttt ttataagc
//
LOCUS       DAPGOF010000170        10727 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.171, whole genome shotgun sequence.
ACCESSION   DAPGOF010000170 DAPGOF010000000
VERSION     DAPGOF010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.171"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(116..1813)
                     /gene="treA"
                     /locus_tag="RHV13_003854"
     CDS             complement(116..1813)
                     /gene="treA"
                     /locus_tag="RHV13_003854"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HDV1243332.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPT
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTIRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWQFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPLSESTTQPVKQKEAEPTP"
     gene            complement(2133..3551)
                     /gene="dhaM"
                     /locus_tag="RHV13_003855"
     CDS             complement(2133..3551)
                     /gene="dhaM"
                     /locus_tag="RHV13_003855"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HDV1243333.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDNCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGANIDKVIFDAMHALEAKREQLGLPSSDTEISDSCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQERLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSAVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            complement(3562..4194)
                     /gene="dhaL"
                     /locus_tag="RHV13_003856"
     CDS             complement(3562..4194)
                     /gene="dhaL"
                     /locus_tag="RHV13_003856"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HDV1243334.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPAALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(4205..5275)
                     /gene="dhaK"
                     /locus_tag="RHV13_003857"
     CDS             complement(4205..5275)
                     /gene="dhaK"
                     /locus_tag="RHV13_003857"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDV1243335.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQVDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIARNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            5503..7422
                     /gene="dhaR"
                     /locus_tag="RHV13_003858"
     CDS             5503..7422
                     /gene="dhaR"
                     /locus_tag="RHV13_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HDV1243336.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(7522..10425)
                     /locus_tag="RHV13_003859"
     CDS             complement(7522..10425)
                     /locus_tag="RHV13_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pertactin family autotransporter"
                     /protein_id="HDV1243337.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAAGTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNDGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HASTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTIPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPDPTPEPDPTPTPTPTPKPTTTADAGGNYLNVGYLL
                     NYVENRTLMQRMGDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKR
                     LSDVMPLYVGLYIGSTHASPDYSGGDGTARSDYMGMYASYMAHNGFYSDLVVKASRQK
                     NSFHVLDSQNNGVNANGTANGLSISLEAGQRFNLTPTGYGFYIEPQTQLTYSHQNEMA
                     MKASNGLNIHLNHYESLLGRASMILGYDITAGKSQLNIYVKTGAIHEFSGDTEYLLNN
                     SREKYSFKGNGWNNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
BASE COUNT         2561 a         2656 c         2692 g         2818 t
ORIGIN      
        1 agaaaataaa ggcatagacc gtagaatggg gggcatttcg tggcgaaaaa agagcaatgc
       61 caattttatg cacataatgg cattgctcga tatacagcct cgatatacag caaagttaag
      121 gcgtaggttc cgcctctttt tgttttaccg gctgtgttgt tgattcactg agtggacgcg
      181 tcgctggaac attgtcacac ggttgctctt tcgggcagat caaatccagc attttcagcg
      241 tcacgccgtt ggtccagcca aagccatcct gtaatggata ttcgccaccg ccgccccccg
      301 ttccggtggt gctgacatca tatttttcca ccagcttttt ctcccggtca taggtgtgct
      361 gaacattggt caggaattgc cagctaatgt ccatcgccac ctctttttgc ccgtagtttt
      421 gtaatccttc tgtcgcgacc cactgcaacg gtgcccagcc atttggcgca tcccattgtt
      481 gcccactttt caccgacgtg gtgttcaggc cgcctggttg cagcagatga gttttcgtcg
      541 ccgtcgccat tttgttggcg cgatctttcg ctgccgcatt gacgtatagc gggaacaggg
      601 cggcagcggt taactgattg cgcactttat gacttttcag gtcgtaatcg gcataccagc
      661 cttgttgatc gttccacagg tatttttcga tccctttttg acgggcattt gccagcgttt
      721 cgtactggtt tgccatcgcg ttatctccgg cagccttgct ggcgcgggcg aggatttttt
      781 ccattttaaa catcaggctg ttcagatcga ccggtacgat gctggtggtg cgtatggtat
      841 ttaactgctg cggattgtcc atccagcgcg agctgaaatc ccagccagac gcagcggcag
      901 agcgcaggtc gcggtaaatt tcagtggcag gtcgattcgg attgcttttg gcggtggcaa
      961 tatcttccac ccatgactct ggtcgtggcg tatcacgatc gtcccagtag cggttgagaa
     1021 gggtaccatc ctgaagtttt acaacgcgtt tttcctgttg tccggcttgc aggttttcaa
     1081 caccgtccat ccagtaagca tattcttttt gcatttgcgg caggtattgc ttcaatgcgg
     1141 catcgccttc atgctgcgcc agtaactcta ccatcagggc aaagaagggc ggttgcgagc
     1201 ggcttaaata gtaactgcgg ttgccgttgg gaatatgacc gtaagtgtct atttcatgag
     1261 caaaattggc caccatatcc gcgactttat cccagtgacc gctttcggca agtcctaaca
     1321 tggtgaagta actgtcccag taatatacct cgcgaaaacg tccgcctggc acgacataag
     1381 gttccggcag cggtaacaga gaatcccatt tttcggtgtt ttcggtagaa cgcgttaata
     1441 ccggccaaag tccgtcaata tgttcgcgca gtgactgtcc ctctggcgga acatatttct
     1501 cgccctctgt cggtagggtg aaattgacgt taacgaaatg gcgcagatca aatccgctct
     1561 ggttttgctg catccgataa tcagcaagga tcatcagcgg atcgctgttc ggcacggcat
     1621 cggcaaaggt tttttggtcc ggaaaaagtt tggcgttttg cacatcatta aacagcggcc
     1681 ctaataaaat atcaggcggc tgtggcgtta ccggtgtttc ttctgcctgc accgatagcg
     1741 cagcgaaaca caaaaagatg caggctggaa ttaacgccat tttttgcggg cgagaaggtg
     1801 cgggggattt catcaatcat tctccttagg cgaaaccgaa taaaacggta ttcaagtatc
     1861 agaaaacctt agttcaggat cgcactagct gcatgatcga aatcacattc tgcgaactga
     1921 caggcacctt agaaaaggga attaaaggga taaataacgg cgaaaaagag tgatcaaata
     1981 acgtctgttt attagccagc cagcggcgtt tggttcgcga ttagcgaaga aaaatcatca
     2041 taaattggtg aaaaatataa caggtagtta aaaaacatta gtgctgagta aattgccgga
     2101 tgacatcaga acgatgccat ccgaacggtg gcttaaccct gacggttgaa acgttgcgtt
     2161 ttaacgtcca gcgttagcgt ttcttctggt tgtatcgcat acagtttctc accctgctgg
     2221 caaatccagc caatccccag ttcacgggcg atcagggcgc tgtgggatac cggacttccg
     2281 gcgctaaggc agataccttt tacaaccgcc ggatccagtt gcagtactgc ggagggataa
     2341 atgttctccg ccagtagaat agttggcgag ttaaactgcg ggagttcttc tttcgtttgg
     2401 gtcaggtgga ccagggtgcg atgcagaaga tcgtccacat caatatagcg agcttgtaga
     2461 tattcatcat ccagttgctg gtattgctgg ctaagttctt taagaacttg ctgccaggca
     2521 tattctgccg tgcaatgttc gtgctgaagg agttcgcttg ccgccgccag cagttccgga
     2581 tcatctaaca gtgtatggtg accagaaaag attgcggcaa tatcgtcaag cccgctggct
     2641 tctgctttcg ctgttaacgt catcagatct aataacgtga agtcaatggc ctggcgtaat
     2701 cgttcttgtt cttcttccac ggtcagggtt gatttggcct gtaccgtaca taaaaccggt
     2761 tgataataaa aggctttacc cgaaacaggc ggaacgggac gcagagtagg tggagcaact
     2821 tcctccgttt caccaaagtt atcttcagcc agctgacgga aagcgatcag tgcctcttca
     2881 gcttctggcc ctttcgcaat caggcgcagc gtatcgttat agcgaacttg tagtaacgca
     2941 atctggttaa tactctccgg tgtgacgcat ttgccgtttt tctccagcaa catatcggca
     3001 ttaaatgtcg ataaggtata aaccagccgg gaggctggac gtacatgcag gccgttacgg
     3061 ttctttatga ccaccgccag agaacgggct tcttcatcgt acgcaggaca tgagtcagag
     3121 atttcagtgt cggaggacgg taaacccagt tgttcacgtt tggcttccag cgcatgcatg
     3181 gcgtcaaaga taactttgtc gatattcgcc cccgaggccg cgctgaccgt tgctgccagt
     3241 gtaccttcga ccaacggcgc agcacacaaa cgtacttttg cggcgatctc gggagccagc
     3301 aattccagcg cagtttcagc actcaataat gcgctaccca tatccatcat gaccagcaca
     3361 tggtcggcat cggcaacaga ttcgatggcc tccatcactt tgacggcatc ggtaccaatg
     3421 ggattttgtg gatcgtcaat tcccgcagca atggcgattt tacaattatc actcattaac
     3481 atctgacggg ctaattcacc gacaccttct cccagtcggc tgctatgtga aactatgacc
     3541 aggtttacca tcaccaattc cttactcttt agcggctaac gccaacattt gcatcataaa
     3601 catcaccgag gttgcgccgg gatcctggtg accaatactg cgttcaccga gataactggc
     3661 gcggcctttg cgggcttgca tcgtaatcgt actttgtgca gcggattcgg cgatgctact
     3721 ggcagcttcg agcgccgccg gaacagagag attttgctcg ctggactgac gtaacgattc
     3781 caccaccggc acccacacat cacacatggt tttatcgcca ggttcggctt tcccgcgact
     3841 gattacgccg tccgcgccat cgcggaacat ctgataaagc tcttccagcg tcaggctttg
     3901 ccgtgcctgg gtcgcctgtg ctgcgcggat aaagaaggta ccgaacagcg gaccactggc
     3961 accaccgacg ctggaaagca gcgtcatacc ggtattcttg agaatgaaac cgatatcttt
     4021 atctgcaata gcagggagtt tttccaccac tttgctaaag cctcggttca tatttagccc
     4081 gtggtcagcg tcgccaatct cgcgatccag tccggtaaga tactcgctct cggtgttgaa
     4141 aatatcgcca caacgagtga gccagttaac aatttgagtt ctgctcagtg acattgcttt
     4201 ctccttattt accccagtta agggccgggg tgtggaccgg ggcgtcccag agtgccagcg
     4261 tttcgtcatc aactttcagt aaggtgattg agaaaccggt catatccagt gaggtgcagt
     4321 acgcgccaat taaattacgc gcgatagtca atcccgcttg ctggcaacgt gtggtcaggc
     4381 ggttatagac gccgtacagc tcagaaagtg gagttgcgcc aagattatta accagcgcaa
     4441 tcaccctgtc gccagactgg agcggttgtt tggtttgtgg ttcttcctgc cagctgcctt
     4501 gttgataatc ccagaaacgc aaagtgcgat ggtatgagcc attttccagc agggtgtcga
     4561 acatttcatc gacggtttga tcaagggaag agaaggggcg gcggtcaata cccggctcac
     4621 catgaatgcc gacgccaaac tccatctcat tatccgccag ggtaaaagaa ggtttgcccg
     4681 cggcaggaac ggtacaggca ccgagagcga tacctattga gtggccttga ttattcaact
     4741 tacgccccag ttccgcacag gcgtccagtg agtcgccacg ctccgccgca gcgcctacga
     4801 gtttttcaat taatacggtg ttggcaacgc cgcgccgccc ggcggtataa agactgtctt
     4861 ttactgcaac gtcgtcatca atgaccacag tggtcacttt tacgccgcta tcgtgcagta
     4921 actcggtcgc tgtttcaaag ttaagaatat cgccggtgta atttttgata atcaacagta
     4981 caccttcgcc gccatcaact tgcatggcgc attcaaagat tttatcgggc gtcggtgagg
     5041 tgaaaatttc gcccggacag gcccccgaaa gcatcccctg accgatataa ccacagtgca
     5101 tcggctcgtg tccgctgccg ccacccgaca gcagggcgac ttttcctgca acaggggcat
     5161 cagctcgggt gacatacacc ggatcctgat gcagtgtcag cgatggatgc gctttcgcca
     5221 gtcctgccag ttgttcgtcc agtacgtctt gcacatcatt gatcaatttt ttcattattt
     5281 tgctccagca attacggtag ggcatggatg atgttcaacg acacggcgac cactgactgc
     5341 cgatgaatcc attgtgcatc aggcaaggga aaagaaaatt agcccaaatt atgtttcata
     5401 gtgaaacata tgctttaatg aatgttccat attgaaactt ttacgtgtat taatacttaa
     5461 aattgcgagc cggaacacct tttgtcataa gggatgcggg atatgagtgg cgcttttaac
     5521 aacgatggtc ggggcatatc tcccttaatt gcaacctcct gggagcgatg caataagctg
     5581 atgaaacggg agacatggaa cgtaccacat caggcccagg gcgtgacatt tgcttctatt
     5641 tatcggcgta agaaagcgat gctgacgctc gggcaggctg cgctggaaga tgcctgggaa
     5701 tatatggcac cgcgagagtg tgcgctgttt atcctcgatg aaaccgcctg cattctcagc
     5761 cgtaatggcg atccgcaaac cttgcagcag ctaagtgcac tgggattcaa tgacggcacg
     5821 tattgcgccg agggaattat tggtacttgt gcgctatcgt tagcggctat ctctggtcag
     5881 gccgtgaaaa cgatggccga tcaacatttc aaacaggcac tctggaactg ggccttttgt
     5941 gcaacgccgt tgtttgacag caagggccga ttgacgggaa caatagcgct ggcgtgtccg
     6001 gttgagcaaa ctaccgcagc tgatttgccg ttgacgttgg caatcgcccg cgaggtcgga
     6061 aatttactgc tgacggacag tttgctcgct gaaactaacc gtcatttaaa tcaacttaat
     6121 gccctgttag aaagtatgga tgatggcgtg attagctggg acgagcaggg taatttgcaa
     6181 tttattaatg cccaggcggc gcgggtcttg cgccttgacg cgacggcaag tcagggacgg
     6241 gcaatcactg aactcttaac gttacccgcc gtattgcaac aagcaataaa acaggcacat
     6301 ccgctcaaac acgtagaagc aacctttgaa agtcagcacc agtttattga tgcggtgata
     6361 acccttaaac cgataataga aacgcaggga accagcttta ttttgttgct ccatcctgtg
     6421 gaacagatgc ggcagttaat gaccagtcaa ttaggaaaag tcagccatac cttcgctcat
     6481 atgccacagg acgatccgca aacccgccgc ttgattcatt ttggtcgcca ggcggcgcgt
     6541 agtagctttc ctgtcctgct ttgtggagaa gagggcgtgg gcaaggcact gctaagtcag
     6601 gcaattcata atgaaagcga gcgtgctgca ggtccttata tcgccgtcaa ttgtgagtta
     6661 tatggtgatg cggcgctggc ggaagaattt attggcggcg atcgcacgga caatgaaaat
     6721 ggccgtctga gtcggctgga actggcgcac ggcggcacgc tgtttcttga aaagattgaa
     6781 tatctggcgg tggagttaca atctgctttg cttcaggtta tcaagcaggg tgttatcact
     6841 cgactggatg cgcggcgttt aataccaatt gatgtcaaag tgattgccac aacgaccgcg
     6901 gacctcgcaa tgctggtgga acaaaatcgt tttagtcgcc agctgtatta cgcgctgcat
     6961 gcatttgaaa ttaccatccc gcctctgcgt atgcggcgtg gcagcattcc ggcgttggtg
     7021 aataacaaat tacgcagtct tgaaaaacgc ttctctacgc ggctgaaaat tgatgacgat
     7081 gccctcgctc gcctggtttc ttgtgcatgg cctggcaacg attttgaact ttacagcgtc
     7141 atcgagaatc ttgctctgag tagtgataat gggcgcattc gcgtcagtga tttgccggaa
     7201 catctgttta ccgagcaggc gacagatgat gtcagcgcca cacgcctttc caccagtctg
     7261 tcatttgcgg aagttgaaaa agaggcaatt attaacgcag cccaggtcac aggcggtcgc
     7321 attcaggaaa tgtcggcttt acttggaatc ggccgcacta cgctgtggag gaaaatgaag
     7381 caacatggca ttgatgcagg gcagtttaag cgccgggtat gaaagacaga gacgatttct
     7441 gatacatcag agtgatctat atttcattcc ggcgcacgct aacatttttc agcatcgttt
     7501 aagggcttgt ctatcccgca cttaaaagct gaagcgatat cctccgttga cttgcttctg
     7561 atcaaagagg ttaccctgcg tgtaatccgc ttcgaggtag aatgtgtgct gtttgttata
     7621 ctgtgcactg actcccacgc cgttattcca gccattacct ttgaaactgt acttctcccg
     7681 ggagttgttc aacagatatt ctgtatcccc tgaaaactcg tggatagcgc cagtcttcac
     7741 atagatattc agctggcttt tacctgcggt gatgtcatat cccagtatca tgctggcacg
     7801 ccccagcagc gattcgtagt gattcagatg tatattgaga ccattactcg ccttcatagc
     7861 catctcattc tggtggctgt atgtgagctg ggtttgcggc tctatataaa acccataacc
     7921 agtaggggtc aggttgaacc tctgcccggc ttccagggag atgctcaatc cattcgcagt
     7981 gccgttggcg ttaacgccgt tgttctgact gtccagtacg tggaaactat ttttctggcg
     8041 cgatgctttt acaacgagat cgctgtaaaa accgttgtgt gccatgtaac tggcgtacat
     8101 tcccatgtag tctgaacgtg cggtaccgtc gccaccgcta tagtccggcg atgcatgtgt
     8161 tgagccaata tacagaccga catacaacgg cattacatca gagagacgtt tatccccacc
     8221 aaactggata ccgctgtaac ccatgtcaaa gccgctcagt ttgccactgg cgaaggagtc
     8281 caggcttccc ccataactgc gcagccagat attaccgtct ttactctgat ttcgcaggtc
     8341 acccatccgt tgcatcagcg tacggttttc aacatagttc aataagtaac caacattgag
     8401 ataattgccg ccagcatctg cggtcgtggt gggtttaggt gtgggagtgg gggtaggcgt
     8461 aggatcgggt tcaggcgtag gatcgggttc aggcgtagga tcggggttga ctatgggagg
     8521 ctgagcggga gccggcgttg gttcaggatt aggagtcggt tccggaattg tcccggaagc
     8581 ataaagctcc cagttagtgc catttttacg cacatcgtac agatatcctc ccaactcaac
     8641 ctgagaagac gcgctgaacg aggccgcgcc gtcagtggtt tttaccactg tcagaacttc
     8701 atttcccgtt gtggcttcgc tgccctggtt acggatagcc aatacatgat taccagcact
     8761 actcccgctg atattcaata aatccccttt attattaacg ccattaccct cgccaacaac
     8821 atcagcacgc ataataaagg tactgttacc gctcaggttc tctacgttta atgtggtaaa
     8881 tgtgccggca gttgatgcgt ggctggcaaa atcgacagtt gaatggctca gcgccagcgt
     8941 gtcgagatta gaattacttg ttacgttcca gacgctgtta ttcattgcaa cgtccagttt
     9001 cccgtcattg acattatcgc taagggagga acctgtccaa accgaccctg ggtgcatatc
     9061 cagattgatt agcccacctt tggagagaac gctaccgttg ataagcatac gacccgaagc
     9121 gttgatgcgg ctggcggcat aaccatcgtc atgttgcgtt gcgatggcca tctggtcggg
     9181 tgtactcata tcaatgacca gatcgctcgt gaggtcgatc tggctgttgg tcatggcata
     9241 aatccccctg gctcctgccg cgccggtgat agccaaactg tctccggtta ttctaccgcc
     9301 gctgagcgcc cacaaaccca gcgccagact gccattacga tcaaccgtaa tatcggtatt
     9361 ttgcatgttg ataacagccg ttgccgtctg ggccgaggca ccataagaac cgccggaaaa
     9421 gatgctgttt cgttgcgctg ccgtgccaga aaaattgatt gtcgcgtctg aaccactggt
     9481 gacgagacca ccgccctgcg cggaagaaat atggctatct gcaccgatag tggttgtacc
     9541 accacgaact tcgacgccat tggccgcggc tccagttaca tcaacagtga gtgcattcgc
     9601 gctaacctgg ccaaagctcc agaggccgtg agcattatcg ccattggttt taatggtact
     9661 gtttgttccg agatcgacaa cagagttttt ctgtacgttt atccccatgg cgctgtagcc
     9721 ctgaacatcg attgtcaggt ctgtcgccgt aaaacgcgca gcaccattgg cgttattgcc
     9781 gttgagacca ccgatataaa cacctgtact tccatcggtc ttgattttac ttccgcttcc
     9841 aagatcgaca ctggtgccat agtcattgat ggttaggcct ataccgttcg agttttcaat
     9901 ggtgaattga gtggctgtta gtgttgagct atgcccaata atgatgccgt catcgttact
     9961 cttaatggtg ctgcctgtgc ctaagtcagc atgggtatag tcaccaatta aattaatgcc
    10021 gatggcagag gtttgaccaa caacatctac tgtgagtcgg ttagctgtca gggagctgtt
    10081 tttgccctga ataatgattc cttttgcata tccagaggcg tcgttgacat tgacggtgac
    10141 atcattaccc aggttgatat tgccaggcgt gtttcctgct ggggtcatta caccatagag
    10201 gtttcctgat ggatcggcgg tatctcctgt aattctgtca ccatcattaa tagtaatatt
    10261 ggtgccatat tgacttcctg taccagcagc tatcagcagg ggggaaaaga taattgcatt
    10321 caaaccaata gcgccaaatt ttatcagttg aattgagggc gaacggattt ttaattcagc
    10381 gagacgatcg gctttggtat tcccattgtg ttgtttgatg cccataccac tcctatatag
    10441 tacccaggct atgggtctat ggattttgtc attttgttta agtcaatgaa taatatcctc
    10501 ttatcatcat gatatttgta tggtgcaatc atgatgagct tctctgagaa taattctcta
    10561 ttaccgggca aaatcaggaa cacaggaatt aatatatatt taacatcccc cttacaaaga
    10621 tgagtttata tcgagttatt ccctaagttt tcgttactta ttccaattcg ttactatgaa
    10681 tatgacaaaa atattacaac aaacagcggt taaaaaattt ggtgaat
//
LOCUS       DAPGOF010000171        10654 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.172, whole genome shotgun sequence.
ACCESSION   DAPGOF010000171 DAPGOF010000000
VERSION     DAPGOF010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10654
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.172"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            150..1217
                     /gene="pilL"
                     /locus_tag="RHV13_003860"
     CDS             150..1217
                     /gene="pilL"
                     /locus_tag="RHV13_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis lipoprotein PilL"
                     /protein_id="HDV1243338.1"
                     /translation="MKKNYLTLILPSLLSGCAGISGSNNVAPSDTPALVFVDGQISES
                     AGVLALTQQRLSPPKITPPPRVSVPETVKTAHPVSESRPLPSPTISQNFSRLIITGKP
                     GSAPVTLSPTARNRTVSQWIKTLLPEGWKFRQENAATPKLNTKLVSWSANDQWTRSLN
                     RLLEEQHLWGHLDWDNKTLTVTTSAAAPAQDTATTISPAAMVTTSTSPESPTTANSQN
                     KPRNPFRGNSVSSSTPAATPTPTGSTVKSIPLMTGTPVKPVSQGKEWRAPAGTTLREN
                     IIKWAEETKCESMASTHWMVIWPLSVTDYRLDAPLMFRGSFESAMEQVFELYRTAQKP
                     LYAQASRMQCIITVSDTRDGR"
     gene            1217..1654
                     /gene="pilM"
                     /locus_tag="RHV13_003861"
     CDS             1217..1654
                     /gene="pilM"
                     /locus_tag="RHV13_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HDV1243339.1"
                     /translation="MGWLVMAVGLTILIITGSYQNQKMSETTNAQQYASASVWASQIL
                     MIANRINDIRYVSGQQDGVISSDKLALPVTPDSRIKHQLQQGRLWVWMPEQPGLVETL
                     RSKSRGSALIGIFQNGQLTWLSGTATGLTPPAGITAGSVVYVN"
     gene            1668..3350
                     /locus_tag="RHV13_003862"
     CDS             1668..3350
                     /locus_tag="RHV13_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HDV1243340.1"
                     /translation="MKKSHQRSMKLAVLPCMIAVALSISGCTFSEINKMQKKAQEDSA
                     HAREKVSALSARKSQALTWLDNQWINPVPVAQVSREKKQTAPACYITQARKGEITLQE
                     LGQRITAVCGIPVIITPDAANSTLEGGATRQMTGTLPAPDENGRLPLSSLGSTTMTTS
                     TQPLTLNNLMWQGDINGLLDLMASRSGLYWRMDNGRIVFYLTETRTYPLHMLNTKTSS
                     SSSVSSGSTSTMGATGGQDNSASGDATSSQSTTVGQEYDLYEDIRKTIEAMLTPEKGR
                     YWLSASSSTLTVTDTPAVQEAVARYVDEQNSIMNRQVALNVQVLSVSNTRNEQFGLDW
                     NLVYKSLHSAGATLNNASGDFTGATSAGVSILDTATGNAAKFSGSSLLIKALSEQGDV
                     SVVTSQESTVTNLTPVPIQMADQTVYVAQSATTTTTDVGATTTLTPGMITTGFNMTLL
                     PLIQKTGNLQLQMNFNLSDPPTIRSFTSKDGNSYIEMPYTKLRSLSQKVNLKEGQSLV
                     VTGFDQNNTTTSKAGTFTPANPLFGGSQTGKNERSTLVIIITPTFPSGGNNG"
     gene            3343..4638
                     /gene="pilO2"
                     /locus_tag="RHV13_003863"
     CDS             3343..4638
                     /gene="pilO2"
                     /locus_tag="RHV13_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HDV1243341.1"
                     /translation="MADEDINPVVLLADPKVNHRVWAACLKWSPVVKKQRVPSHQKHK
                     PHVKSRRLTSLKVTVGSRSSRGKISRITGTGILARPERNHYFSLALAFCSWVRNGYGV
                     FRYSDKELLFLASINGQPAVMADLSGNDADVAQKVSLFLTMNEEPPEKWQVVSPLEHP
                     DNWESIITRLSSADLRRCKLTVGNRSKFTLPAVLFLVAASAGTVFWMTQPEPDVVPTP
                     EEIAARARLQFKKPDPPPELPHPWASQPVISDFLKACADLRKPSPVALEGWKLTGGTC
                     TPETFTLIYERQPGGTIEGFLARSKEIFNVIPDFNLKDGARLASVTRPLPSLPRRDEA
                     VPTPSEQLMRVFTWFQKKQLTPAINEIAIPEPLPGNDGEPAPVQKWKEYQFSLSTPVN
                     PDELFPLFQDTGVRLSNIHFELNGGTFSYSSEGHIYASR"
     gene            4625..5077
                     /gene="pilP"
                     /locus_tag="RHV13_003864"
     CDS             4625..5077
                     /gene="pilP"
                     /locus_tag="RHV13_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HDV1243342.1"
                     /translation="MRPGKLLIIPSIFFFSGFSFATTQPLVTIGELEAQQNRNILLQA
                     KVQGAQLQKQLEESDVTSSSETVSGFSGVTASLPSVSEQPTSKNRKELPVIMEINGKD
                     KRLNAVLRMADGRQTSVTTGSQLPGTSVTVKSISLSGVTLSDGTTLTF"
     gene            5088..6641
                     /gene="tadA"
                     /locus_tag="RHV13_003865"
     CDS             5088..6641
                     /gene="tadA"
                     /locus_tag="RHV13_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HDV1243343.1"
                     /translation="MDKYNLKDALLFISRGDTHEILIETNQRTRPDVQSNLQELLKLY
                     PDINPKVVSLSELQEAGQDEKNRGPKERIIHLKDLADVSESEKKVLSYFETARKLGAS
                     DIHFLISESIFKVRMRIFGELQTVDEDQPALGYSLCATAILSMADVTETSFFPQREQD
                     ARLSPQLMRKIGIFGARYSHRPTGDGLIAVMRLIPDDGDKVPTFKQLGFIPEQIRLLN
                     IMLRRPEGKIVLSGPTGSGKSTTLRSACRVYLDDNQGRHLLTIEDPLEGQILGATQTP
                     IICDKSDEDAVKLAWSRAISSAMRLDPDAIMEGEMRDLISMMSTTYAAQTGHIVLTTL
                     HTNSALGIPERMITMGMNADLICDAQLLIGMISQRLVPTLCPSCRIPWEKRAPELSDD
                     ERDYLERHCNKDSLCSTDNIWFRNHHGCSECNHDVIINGRKRGEIGKGLTGRTVIAEV
                     IEPDNRLFQILKTRGKVAARKYWLENMKGISRVEHLLRRINEGLVDPLEADRIIPLDE
                     DERLSIDDV"
     gene            6654..7751
                     /locus_tag="RHV13_003866"
     CDS             6654..7751
                     /locus_tag="RHV13_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001699973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDV1243344.1"
                     /translation="MREMNFSQRLRRFIVRKTFSAPYRVQFYEALRFLLENKQPLKTA
                     LEQMRDAWTDFGRKWHPFAELATDCIESLRENSGENSLEYTLSLWVPQEEAAVISAGI
                     RSGSIVDALQFATTLTDAKEQIHQAIWQMAIYPVGLLIMMTGTLYVLNTELIPELSKI
                     SSPDSWSGALGFLYGLSVFVDNYGAICAVLFAVITGLISWSLPNWKSPDSVRTFADKI
                     MPWSIYQDIQGATFLLNMAALLKAKMTTLNSLNILQEFASPWLSTRLDSIIYRVRQGD
                     HLGLALRQCGYQFPSREAANFLSLLQGDGATELISNYGQRWLSQTLQRVKKRANVIRL
                     IMLIFLVMSLMLLVFAIMDIQSISDNSMGNF"
     gene            7769..8383
                     /locus_tag="RHV13_003867"
     CDS             7769..8383
                     /locus_tag="RHV13_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilX"
                     /protein_id="HDV1243345.1"
                     /translation="MLVENINTTLTGNNKKNEPHDKGWAILEQGTIALVVLFVIVVVL
                     GSLYALRTRANVATETANIQTIITSAQSLLKGSDGYTFTSSAKMTGALIQMGVIPSGM
                     TVQGDKTSGTATLYNAWGGAVTVAPASTSGFNNGFTVTYDKVPQDACIQIATRISKTG
                     LTNGITLNSTAHSDGKVTTEEASTQCKADNGSTGTNKLIFTING"
     gene            8393..8953
                     /locus_tag="RHV13_003868"
     CDS             8393..8953
                     /locus_tag="RHV13_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HDV1243346.1"
                     /translation="MSGGTVPLRLISLILLFSLIPPARASYVAPKQIWTNKWDNCFAA
                     AGARYQIEPLLLKAISAGESSLRPGAININKDKKTGKASSTDYGLMQINSTHIPKLIN
                     MGVIKKSEDLITKPCLNIHIGSWILARHFQICGVSWNCLGSYNAGFRKDRHETREQYA
                     NKIWRIYRDMKGICLPGQGGRQCRQS"
     gene            8938..9594
                     /locus_tag="RHV13_003869"
     CDS             8938..9594
                     /locus_tag="RHV13_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDV1243347.1"
                     /translation="MQTIVGISLSPVYILPLMFTFSIIGRTLLFRVRYFLSDTGHLWY
                     KTHPAVLSGIWLYSIAVALIILSSSPLLYRIHAVLILSFILQMAVTDALTGLLPGTFT
                     RRFLIAGMLSQITTDIWWFRTTEFATAAIVLFCLHKLVNRHRLNIGTGDLWLIAGITA
                     WSGLYNAIWCVLLGTGGFVLWHSTWCIKGHKEGPLGPWLCFGHVLLLLDNLYQPLWVI
                     "
     gene            9594..>10654
                     /gene="pilV"
                     /locus_tag="RHV13_003870"
     CDS             9594..>10654
                     /gene="pilV"
                     /locus_tag="RHV13_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HDV1243348.1"
                     /translation="MKKYDRGWASLETGAALLIVMLLIAWGAGIWQDYIQTKGWQTEA
                     RLVSNWTSAARSYIGKNYTTLQGSSTTTTPAVITTTMLKNTGFLSSGFTETNSEGQRL
                     QAYVVRNAQNPELLQAMVVSSGGTPYPVKALIQMAKDITTGLGGYIQDGKTATGALRS
                     WSVALSNYGAKSGNGHIAVLLSTDELSGAAEDTDRLYRFQVNGRPDLNKMHTAIDMGS
                     NNLNNVGAVNAQTGNFSGNVNGVNGTFSGQVKGNSGNFDVNVTAGGDIRSNNGWLITR
                     NSKGWLNETHGGGFYMSDGSWVRSVNNKGIYTGGQVKGGTVRADGRLYTGEYLQLERT
                     AVAGASCSPNGLVGRDNTGA"
BASE COUNT         3047 a         2536 c         2456 g         2615 t
ORIGIN      
        1 atgaaagatt atcaattcct tttgtatatc tcttatttca gtggtctctc ccgcatttgc
       61 attcggacgg aatgacagtc gtcattcgtc ctctccagcc gcctcacacc aattaacaaa
      121 cacgtatata cgaacacgga gtctccgtta tgaaaaaaaa ctatctgaca cttatactgc
      181 cgtcattatt gtccggctgt gccggaattt ccggcagcaa taacgtggca ccatcagata
      241 cgcctgctct ggtttttgtt gacggacaaa tcagtgagag tgctggcgtt ctggcactta
      301 cgcaacaacg tctttctcca cctaaaataa ctcccccccc gagagtcagc gtgccagaga
      361 cagtaaaaac agcacatcca gtcagcgaat cccgaccgtt accttcacca acaataagtc
      421 agaatttctc ccggttaatc attacaggca agcctggtag cgcccctgta acactatcgc
      481 cgacggcccg taaccggacc gtcagtcagt ggataaaaac attactaccg gaaggatgga
      541 aattcagaca agagaatgca gccacgccca agctgaatac caaactggtt tcatggtctg
      601 ccaatgatca gtggacccgt tctctgaatc ggttgctgga agaacaacac ctatggggcc
      661 atctggactg ggataataaa acgttgaccg tcacaacatc agctgctgca ccggcacagg
      721 acacggcaac cactatctcg ccagcagcca tggtaaccac atccacttcc cctgaatctc
      781 caacaacagc aaatagccag aacaaaccac gaaatccatt cagaggaaac agcgtatctt
      841 cgtcaacccc cgccgccacc ccgactccaa ccggctccac agtcaagtct atcccgctca
      901 tgaccggaac gcctgttaaa ccggtatctc agggtaaaga gtggcgtgcg ccggcaggca
      961 ccacattaag agagaacatt atcaagtggg cagaagaaac aaaatgcgaa agcatggcat
     1021 caacgcactg gatggtcata tggccacttt ctgtaaccga ttaccgtctg gatgcgcctt
     1081 taatgttccg gggatcattt gaatcagcca tggagcaggt atttgagtta tatcggactg
     1141 cgcaaaaacc tttatacgca caggcttctc gcatgcagtg catcattaca gtttctgata
     1201 caagggatgg tcgctaatgg gatggctggt catggccgtc ggcctgacca ttctgattat
     1261 cactggtagc taccagaatc agaagatgtc tgaaaccaca aacgcccagc aatatgcaag
     1321 tgcgtctgta tgggcaagtc agatactgat gattgctaac agaatcaatg atatcagata
     1381 cgtatcaggc cagcaggacg gagtaatttc ctcagataaa ctggctctcc ctgtcactcc
     1441 ggactcccga attaaacatc agttacaaca ggggcgtctg tgggtatgga tgcctgaaca
     1501 accaggtctt gttgaaactt tgcgttcaaa aagtcgtggt tctgcactaa tagggatctt
     1561 ccaaaacggg caactcacct ggctatcagg aactgcaacc ggtctgacac ccccggcagg
     1621 cattactgct ggttctgtcg tctacgttaa ctaatctgga tatattcatg aaaaaatcac
     1681 accagcgttc aatgaagctg gcggtgctcc cctgcatgat cgccgtcgct ctttccatct
     1741 ccggatgtac tttcagcgaa atcaacaaaa tgcagaaaaa agcacaggaa gactcagcac
     1801 atgcacggga aaaggtatca gccctttcgg cccgtaaatc gcaggctctt acctggctcg
     1861 ataatcaatg gataaaccct gttccggtcg ctcaggtatc aagagagaaa aaacaaacag
     1921 ctccggcctg ctacatcacg caggcaagaa aaggagagat cactctgcag gaactggggc
     1981 aacgtattac tgccgtatgc ggcatccctg tgatcatcac gcctgacgca gccaattcaa
     2041 ctcttgaagg aggcgctacc cgccagatga caggaacact accagcacca gatgaaaatg
     2101 ggcgtctacc gttaagcagc ctgggcagca caacaatgac tacctccact cagccattaa
     2161 cgctgaataa cctcatgtgg cagggagata tcaatggtct tctggatctg atggccagcc
     2221 ggagtggtct gtactggcgc atggataatg gtcggattgt attctatctg actgaaacca
     2281 gaacgtatcc acttcatatg ctgaacacca aaaccagcag cagttccagt gtcagctctg
     2341 gctcaacaag cacaatgggg gcaacaggag gccaggataa ctcagcatcc ggtgatgcaa
     2401 cgtcctctca gagcacaacc gttggtcagg aatacgatct gtatgaagac atccggaaaa
     2461 ctattgaagc aatgctgaca ccagaaaaag gccgttactg gttatctgca tcgagctcaa
     2521 cgctgactgt cactgatact ccagctgtcc aggaagccgt cgcacgatat gtggacgaac
     2581 aaaacagtat tatgaaccgc caggtagccc tgaacgtaca ggttctgagc gtcagcaata
     2641 ccagaaacga acagttcggt ctggactgga accttgttta taaatcgcta cattccgccg
     2701 gagcaacgtt gaacaatgca agcggagatt ttacaggcgc tacatctgca ggcgtatcaa
     2761 ttctggatac ggcaacaggg aatgccgcca aattcagcgg ttccagtctt ctgattaaag
     2821 cgctgagtga acagggcgat gtcagtgttg tgacttcaca agaaagcact gtcacaaacc
     2881 tgacgccggt acctatccag atggcagatc agacggttta cgtcgcccag tcagcaacaa
     2941 caacgactac ggatgtagga gcaacaacaa cattaacgcc gggcatgatc accaccggat
     3001 tcaatatgac cctgctgcct ttaattcaga aaacgggcaa tctccagttg cagatgaatt
     3061 ttaatctgtc agatccccca acaatccgta gctttacgtc aaaagacgga aacagttaca
     3121 tcgaaatgcc gtataccaaa ctgcgttcac tgagccagaa ggtcaatctg aaagaagggc
     3181 aatcacttgt cgttactggt ttcgatcaga acaatacgac gacaagtaaa gccggtacgt
     3241 ttacgccagc aaatccatta tttggtggtt cacaaaccgg gaaaaatgaa cgcagcacgc
     3301 ttgtaatcat cattaccccg actttcccgt caggaggcaa caatggctga tgaagatatc
     3361 aacccggttg tacttctcgc agatcctaaa gtaaatcatc gcgtgtgggc agcctgtctt
     3421 aaatggagtc ctgtcgtaaa aaaacagcga gtcccttctc atcagaaaca caagccccat
     3481 gtaaaatcca gacggcttac ctctctgaaa gtgactgtag gcagcagaag ttcccgtggg
     3541 aagatcagcc gcattaccgg cacaggaata ctggccaggc cggagcgcaa tcattatttt
     3601 tcccttgctc tggctttctg ctcatgggtg agaaatggat acggcgtctt ccggtacagc
     3661 gataaggagc ttttattcct tgcctccatt aatggccagc ctgccgttat ggctgatctg
     3721 tcaggaaatg atgctgatgt tgctcaaaag gtcagtcttt ttctgacgat gaacgaagag
     3781 ccccccgaaa aatggcaggt cgtatctcct ctggaacatc ctgataactg ggaaagtatt
     3841 attacccggt tatcatcagc agatctccgc cgttgcaaac ttactgtcgg caatcgcagc
     3901 aaattcacac tgcctgccgt acttttttta gtagcagcca gcgcaggcac tgtattctgg
     3961 atgactcagc cagagccgga tgtcgtaccg acgcccgagg agatagctgc acgggcaaga
     4021 ttgcagttta aaaagcccga tccaccgcca gaactcccgc atccatgggc atcacaacct
     4081 gtaatcagtg attttctgaa agcctgcgcg gaccttcgaa aaccatcacc ggtcgcgcta
     4141 gaaggctgga aacttaccgg tggaacatgt actcccgaga cattcacact gatttacgaa
     4201 cgacagccag gaggaactat tgaaggcttt ctggcaagaa gcaaagaaat ctttaatgtt
     4261 attcctgact ttaaccttaa agatggtgca cgcctggctt ctgtaacccg gccactgcct
     4321 tccctgcccc gcagggatga ggcagtccct accccctcag aacaactgat gcgggtgttc
     4381 acgtggtttc agaaaaaaca gttaacgccg gccattaatg aaatagccat accggaacca
     4441 ttaccaggta acgatggtga accagcccct gttcagaaat ggaaagaata tcagttcagt
     4501 ttatcaacgc ctgtaaatcc tgatgagttg ttccctttat ttcaggatac cggcgttcgt
     4561 ctgagcaata tccattttga attaaacggc ggtactttca gttattcctc agaaggtcat
     4621 atttatgcgt ccaggtaaat tactcattat tccatccata tttttcttct ccggtttttc
     4681 atttgcaaca acacaacctc ttgtcacaat aggagaactt gaagcacaac aaaaccggaa
     4741 tattctttta caggcaaaag ttcagggcgc acagctacag aaacaactcg aagaatcaga
     4801 tgttacttca tcatctgaaa ctgtttcagg tttctctggt gtgactgcct ctttgccatc
     4861 agtttcagag cagccgacat ctaaaaacag aaaggaattg cccgttatta tggaaataaa
     4921 cggcaaagat aaacgtctta atgccgttct tcgcatggct gatggacgtc agacaagcgt
     4981 caccactggc agccagcttc caggcacatc cgtcacagtg aagtcgatct cattgtctgg
     5041 cgtcacgtta agtgatggca ccacccttac tttctgagta acgtgatatg gataaatata
     5101 atctgaagga tgctctcctt tttatatcca ggggagacac ccatgaaatt cttatagaaa
     5161 caaaccagcg gacaaggcct gacgtacaaa gtaatctcca ggagctgcta aaattatatc
     5221 cggatatcaa cccaaaagta gtctcgttgt cggagttaca ggaggcaggt caggatgaga
     5281 aaaacagagg cccgaaagaa cgaattattc atctgaaaga ccttgctgat gtcagtgaaa
     5341 gtgagaagaa agttctatct tattttgaaa ctgccagaaa acttggtgct tcagatattc
     5401 atttcttaat ttcagagtct attttcaaag ttcgcatgag aatattcggt gaactgcaga
     5461 ctgtagatga agatcaacca gcactgggct attcactttg cgcaacagcc attctgtcaa
     5521 tggctgatgt cactgaaacc agtttcttcc ctcaaaggga gcaggatgcg cgcctgtccc
     5581 cacaattaat gcgaaaaata ggaatatttg gtgcacgcta cagtcacaga cctacgggag
     5641 acggtctgat tgcagttatg cgcctgatac ccgatgacgg agataaagtt cccacgttta
     5701 aacaactggg ctttatcccg gaacaaatcc ggttgctgaa cataatgctg cgccgcccgg
     5761 aaggaaaaat agtcctgtca ggtccaaccg gttccggtaa atcgacaacg ttacgcagcg
     5821 cctgccgtgt atatcttgac gataatcagg ggcgacacct gctaacaatc gaagatccgc
     5881 tggaaggaca gatactggga gcaacacaga ctccgattat ctgcgacaaa tccgacgaag
     5941 atgccgtcaa actggcatgg agtcgggcta tttcatcggc aatgcgactt gaccctgatg
     6001 ccatcatgga aggggaaatg cgcgatctta tctcgatgat gtcaaccacc tacgcggcac
     6061 agaccgggca catagttctg acgacattac atacaaactc agctctgggt ataccggagc
     6121 gaatgattac gatgggtatg aatgccgatc ttatttgcga tgcgcaatta ctgattggga
     6181 tgatcagcca gcgccttgta ccaactctgt gtccctcctg ccgtattcca tgggaaaagc
     6241 gagcacctga acttagcgac gacgagcgtg attaccttga gcggcactgc aataaagatt
     6301 cactctgcag tacagataat atctggttcc gaaatcatca cggatgctct gaatgtaatc
     6361 acgacgtaat cattaacggc agaaagcgcg gtgaaatagg taaaggcctc accggcagga
     6421 ctgtaattgc tgaggtgata gaacctgata accgcttatt tcagattctg aaaacacgcg
     6481 gcaaagttgc agcacgtaaa tactggctgg aaaacatgaa aggcatcagt cgtgtagagc
     6541 atcttcttcg ccgtatcaat gaaggtctgg tagaccctct tgaagccgac agaatcatcc
     6601 ctctggacga agacgagcgc ctcagcatcg atgatgtcta acaggagaac aacatgcgtg
     6661 aaatgaattt cagtcaacgg ctgaggcgtt tcattgtcag aaaaacgttc agcgccccct
     6721 acagggtgca gttttacgaa gccctgcgtt ttctgctgga aaataaacag ccattaaaga
     6781 ccgcactgga acagatgcgg gatgcctgga cagactttgg acgaaaatgg catccctttg
     6841 ctgaactggc cacagactgc attgagtctc ttcgtgaaaa cagtggcgaa aactctctgg
     6901 aatatactct tagtttatgg gttcctcagg aagaggccgc agtaatcagc gccgggataa
     6961 gaagtggttc aattgtggat gcactgcaat tcgccaccac tcttacagat gcaaaagaac
     7021 aaatccatca ggctatatgg caaatggcta tttatccggt gggattgtta atcatgatga
     7081 cggggacatt atatgttctg aatacagagc taatacctga actgagcaaa ataagctccc
     7141 ccgattcatg gagtggtgct cttggttttc tttatggatt atctgttttt gtcgataatt
     7201 acggcgctat atgtgccgtt ctttttgcag tgatcacagg tttaatatca tggtctctgc
     7261 caaactggaa aagccctgac agtgttcgta cttttgccga caagataatg ccatggagta
     7321 tttatcagga tatacaggga gcaacatttc tcctgaatat ggctgcactt ctgaaagcca
     7381 aaatgacgac gctcaactcg ctaaacattc ttcaggagtt tgcatcacca tggttaagca
     7441 cacgtcttga cagcattatt taccgcgtcc gccaggggga tcatcttgga ctggctttgc
     7501 gtcagtgcgg ttatcagttc ccatccaggg aagcagccaa cttcctgtct ttattacagg
     7561 gagatggcgc aacagagcta atcagcaatt atggtcaacg atggcttagt caaacacttc
     7621 aacgtgtaaa gaaacgggca aacgtgattc gtttaattat gctgatattt cttgtcatgt
     7681 ctctcatgtt actggttttt gccattatgg atattcagtc tattagtgac aacagcatgg
     7741 gtaactttta aacaaaacga ggatatttat gttagtagaa aatattaata ccactttaac
     7801 tggaaataat aagaagaatg agcctcatga taaaggatgg gcaattttgg agcagggaac
     7861 aatagcgctc gttgttcttt ttgttatagt cgttgttctg ggtagtttgt atgcgttgcg
     7921 tacaagagcc aatgtggcaa ctgaaaccgc aaatatacaa acaattatca caagtgccca
     7981 gagcctgcta aaaggtagcg atggatatac atttaccagc agtgccaaaa tgacaggtgc
     8041 cctcattcag atgggtgtca ttccctctgg gatgaccgta cagggcgaca aaacatctgg
     8101 tacagcaacg ctctataacg catggggcgg tgctgtaact gttgccccgg cttcaacatc
     8161 agggttcaac aacggtttta ccgttaccta tgataaagtc ccacaggatg cctgtatcca
     8221 gattgccaca cgaatcagta aaactggttt aactaacggc attaccctga acagtactgc
     8281 tcacagtgat ggaaaagtca ctactgaaga agccagtacg caatgcaagg cagataatgg
     8341 cagcactggt acaaacaaac ttatcttcac catcaatggt taaggagttt taatgtcagg
     8401 gggtacagta cccctcagac ttatttcatt aatcctgctt ttttctctta ttccacccgc
     8461 ccgagcttca tacgtagctc ccaaacagat ctggaccaac aagtgggaca attgctttgc
     8521 cgctgccggc gcgcgctatc aaattgaacc attattattg aaggctattt ctgccgggga
     8581 atcttcgttg aggccgggcg caattaatat aaataaggac aaaaaaacag gtaaagccag
     8641 tagcacggat tatgggttga tgcaaataaa ctcaacccat attccaaagc tgataaatat
     8701 gggcgtaata aaaaagtctg aggatttaat taccaaacca tgccttaata tccatatagg
     8761 aagctggata ctggcaagac attttcagat atgtggcgtc agctggaatt gccttgggtc
     8821 atacaatgcc ggattccgta aggacagaca tgaaacaaga gagcaatatg ccaataaaat
     8881 atggagaatt tatcgtgaca tgaaagggat atgtcttccc ggacagggag gcaggcaatg
     8941 cagacaatcg taggcataag tctatcccct gtttatatac tgccattgat gttcacattt
     9001 tccattattg gacgaactct gctattcaga gttcgttatt ttttatctga tacgggacat
     9061 ctctggtata agacacaccc tgctgttctg tcaggaatat ggctatacag tatagctgtg
     9121 gcgttaatta ttttatcatc atcaccactg ctctaccgta ttcatgctgt ccttattctt
     9181 tcgttcatac tgcaaatggc ggtaacggat gcactgacag gtttattacc aggaacattc
     9241 acccgacgtt ttttaattgc aggaatgcta tcacaaatca cgactgatat atggtggttc
     9301 cgtacaacag aatttgccac tgctgcaatt gtcttattct gccttcataa attagttaac
     9361 cgccatcgtc ttaatattgg cactggagac ctgtggctta ttgcaggtat taccgcatgg
     9421 tcagggttat ataacgccat atggtgtgtg ttgctgggca caggaggatt tgtcttatgg
     9481 cattcaacat ggtgcataaa agggcataaa gaaggccctc tgggtccatg gctatgtttt
     9541 ggccatgtcc ttttgctgct ggataatctt tatcaaccac tatgggtaat ctgatgaaaa
     9601 aatatgaccg aggctgggca tcgctggaaa ctggcgcggc tttactgatt gttatgctcc
     9661 tgattgcctg gggagcgggc atatggcagg actatattca aacaaaagga tggcaaactg
     9721 aagcacgtct tgtcagcaac tggaccagtg cggcccgctc ttatataggg aaaaactaca
     9781 cgactctcca gggcagtagt accacaacaa ctcctgccgt tatcacgaca accatgctga
     9841 aaaataccgg ctttttgtcc agcgggttta ctgagacaaa cagcgagggg cagcggttac
     9901 aggcatatgt ggttcgaaac gcccaaaacc cggaattact acaggcaatg gttgtatcca
     9961 gtggtggcac accttatcca gtgaaagcac ttatccagat ggctaaggat attaccactg
    10021 gtcttggtgg atatatccag gacggcaaaa cagccacagg tgcattacgt tcctggtcag
    10081 tagctttaag taattatggt gccaaaagcg gtaacgggca tattgccgta ttgttatcga
    10141 cagatgaact tagtggtgca gctgaggaca ctgatcgtct ttacagattc caggtcaatg
    10201 gtcgccctga cttaaacaaa atgcacacgg ccattgatat gggatcaaat aacctgaata
    10261 acgttggggc agtaaatgcc cagacaggta atttcagcgg caatgtgaat ggtgtaaatg
    10321 gcactttcag cggtcaggta aaaggcaata gcggaaactt tgacgtaaat gtcaccgctg
    10381 gcggtgatat cagaagtaat aatggttggt taattactcg taacagtaaa ggctggctca
    10441 atgaaactca cggtggcgga ttttatatgt ccgatggatc atgggttcga agtgtaaaca
    10501 acaagggcat ctataccggc ggtcaggtga aaggcggtac tgttcgggct gatggtcgcc
    10561 tttatactgg tgaatactta caactggaaa gaactgccgt tgctggcgca tcatgttcgc
    10621 ctaacggcct tgtaggccgc gataatacag gggc
//
LOCUS       DAPGOF010000172        10512 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.173, whole genome shotgun sequence.
ACCESSION   DAPGOF010000172 DAPGOF010000000
VERSION     DAPGOF010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.173"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            143..718
                     /gene="ssuE"
                     /locus_tag="RHV13_003871"
     CDS             143..718
                     /gene="ssuE"
                     /locus_tag="RHV13_003871"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HDV1243349.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQTDGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHRPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            711..1670
                     /gene="ssuA"
                     /locus_tag="RHV13_003872"
     CDS             711..1670
                     /gene="ssuA"
                     /locus_tag="RHV13_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HDV1243350.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQTKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1667..2812
                     /gene="ssuD"
                     /locus_tag="RHV13_003873"
     CDS             1667..2812
                     /gene="ssuD"
                     /locus_tag="RHV13_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HDV1243351.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     AVFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVDFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKATAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2824..3615
                     /gene="ssuC"
                     /locus_tag="RHV13_003874"
     CDS             2824..3615
                     /gene="ssuC"
                     /locus_tag="RHV13_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HDV1243352.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3612..4379
                     /gene="ssuB"
                     /locus_tag="RHV13_003875"
     CDS             3612..4379
                     /gene="ssuB"
                     /locus_tag="RHV13_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HDV1243353.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(4586..7198)
                     /gene="pepN"
                     /locus_tag="RHV13_003876"
     CDS             complement(4586..7198)
                     /gene="pepN"
                     /locus_tag="RHV13_003876"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HDV1243354.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLCLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7464..8666
                     /gene="pncB"
                     /locus_tag="RHV13_003877"
     CDS             7464..8666
                     /gene="pncB"
                     /locus_tag="RHV13_003877"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDV1243355.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYKKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8835..10235
                     /gene="asnS"
                     /locus_tag="RHV13_003878"
     CDS             8835..10235
                     /gene="asnS"
                     /locus_tag="RHV13_003878"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDV1243356.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2374 a         2715 c         2824 g         2599 t
ORIGIN      
        1 gatatataaa gccctttctt tagtttattt tcagaaaaag atacacaaaa tttatatttg
       61 gcattttctt gtctctccga cagactgacc gaattgttat caatgttaac aaaaaaagaa
      121 caattggtta taaggagaga gtatgcgtgt catcaccctg gcgggtagtc ctcgctttcc
      181 ttctcgctcc agctccttgc tggaatatgc gcgggaaaaa ctaaatggcc tggatgtaga
      241 ggtttatcac tggaatctgc aaaacttcgc cccggaagat ctgctttatg ctcgtttcga
      301 tagtccggca ctcaagacct tcaccgaaca gctgcaacag actgatggac tgatagtcgc
      361 tacacctgtg tataaagccg cctattccgg cgcgctgaaa accctgctcg acctgctgcc
      421 agagcgcgct ttgcaaggca aagtggtgct accgctggcg acgggcggta ccgtggccca
      481 tctgctggcg gtcgattatg cccttaaacc agttttaagc gcactgaaag ctcaggagat
      541 cctgcacggc gtgtttgccg atgactcaca agtaattgat taccatcaca gaccccagtt
      601 cacgccaaat ctgcaaaccc gtcttgatac cgcgctagaa actttctggc aggcattgca
      661 ccgccgcgat gttcaggttc ctgaccttct gtctctgcga ggtaatgccc atgcgtaaca
      721 tcattaaact ggcgctggcg ggattgctta gcgtctctac ttttgcggtt gctgcagaat
      781 cctcgcctga agcgttacgt ataggctatc agaaaggcag tattggtatg gtgctggcaa
      841 aaagccacca gttactggaa aaacgctatc cgcaaacaaa aatctcgtgg gtggagttcc
      901 ccgctggccc gcaaatgctt gaggcgttaa acgttggcag tattgatctc ggcagtaccg
      961 gggatattcc gccaatcttt gcccaggctg ccggggctga tttggtatat gtgggcgtcg
     1021 agccaccgaa gcccaaagcc gaagtgattc tggtggcaga aaacagcccg atcaaaaccg
     1081 tagccgatct taaaggtcac aaagttgcct ttcagaaagg ttccagttca cacaaccttt
     1141 tactgcgtgc actgcgtcag gccggactta aattcaccga tatccagccc acttacctga
     1201 cgcctgccga tgcccgcgcc gcgttccagc aaggtaacgt tgacgcctgg gctatctggg
     1261 atccctacta ctccgctgcg ttattacagg gcggcgtgcg ggtgctgaaa gacggcaccg
     1321 atctcaatca aactggatcg ttttatctgg cagctcgtcc ctatgcagaa aaaaacggcg
     1381 cttttattca gggtgtactg gcaaccttta gtgaggccga tgcgttaacc cgcagccagc
     1441 gcgagcaaag catcgcttta ctggcaaaaa cgatgggctt accggcaccg gtgattgcct
     1501 cttacttaga tcatcgccct cctaccacca tcaaaccggt taacgccgag gttgccgcct
     1561 tacagcagca aacggcagat ctgttttatg aaaaccgtct ggtgccgaaa aaagtcgata
     1621 ttcgccaacg catctggcag cccactcaac tggaaggaaa acaattatga gtctgaatat
     1681 gttctggttt ttaccgaccc atggtgacgg gcattatctg ggaacggaag aaggttcacg
     1741 cccggttgat catggttatc tgcaacaaat tgcgcaagcg gcggatcgtc ttggctatac
     1801 cggtgtgctg atcccaacgg ggcgctcctg cgaagatgcg tggctggttg ccgcatcgat
     1861 gatcccggtg acgcagcggc tgaagtttct tgtcgccctg cgtcccagcg taacctcacc
     1921 taccgttgcc gcccgccagg ccgccacgct tgaccgtctc tcaaatggac gtgcggtgtt
     1981 taacctggtc acaggcagcg atccacaaga actggcaggc gacggcgtgt tccttgatca
     2041 tagtgagcgc tacgaagcct cggcggaatt tacccaggtc tggcggcgct tattgctggg
     2101 ggaaaccgtc gatttcaacg gtaaacatat tcatgtgcgg ggagccaaac tgctcttccc
     2161 gccgattcaa cagccgtatc ctccgcttta cttcggcgga tcgtcagacg tcgcccagga
     2221 gctggcggca gaacaggttg atctctacct cacctggggc gaaccgccgg aactggttaa
     2281 agagaaaatc gaacaagtgc gggcgaaagc taccgcgcat ggacgcaaaa ttcgtttcgg
     2341 cattcgtctg catgtgattg ttcgtgaaac taacgacgaa gcgtggcagg ccgccgagcg
     2401 gttaatctcg catcttgatg atgagactat cgccaaagca caagccgcat tcgcccgcac
     2461 ggattccgtg gggcaacagc gaatggcagc gctacataac ggcaagcgcg acaatctgga
     2521 gatcagcccc aatttatggg cgggcgttgg cttagtgcgc ggcggtgccg ggacggcgct
     2581 ggtgggcgat ggtcctacag tcgctgcgcg aatcaacgaa tatgccgcgc ttggcatcga
     2641 cagctttgtg ctttcgggct atccgcatct ggaagaagcg tatcgggtcg gcgagttgct
     2701 gttcccacat ctggatgtcg ccattccgga aattccccag ccgcagccac tgaatccgca
     2761 aggcgaagcg gtggcgaatg attttatccc ccgtaaagtc gcgcaaagct aaggaacacg
     2821 atgatggcaa cgccagtgaa gaagtggtta ttgcgcgttg ccccctggtt tttaccggtg
     2881 ggcatcgtgg cggtgtggca actggcctcc tcggttggct ggctgtcgac gcgtattttg
     2941 ccttcaccgg aaggagtggt gacggcgttc tggacgctct ccgccagcgg cgaactgtgg
     3001 cagcatctgg cgatcagctc ctggcgggcg ctgattggct tttcaattgg cggatcgctg
     3061 gggttgattt tgggactgat tagcgggcta tctcgctggg gagaacggct gctggatacc
     3121 tcaattcaga tgttgcgcaa cgtgccgcat ctggcgctga tcccgctggt gattttgtgg
     3181 tttggcattg atgaatccgc aaaaatcttt ctggtggcgc tcggtacgct attccccatt
     3241 tatatcaaca cctggcatgg catccgtaat atcgatcgcg ggttggtgga gatggcgcgt
     3301 agctatggat tatccggcat accgctgttt atccatgtga tcctgcctgg tgccctgccc
     3361 tcaattatgg tcggcgtgcg ttttgcgttg ggcctgatgt ggctgacgct gattgttgcc
     3421 gaaaccattt ctgccaattc cggcattggt tatctggcga tgaatgcgcg ggagtttttg
     3481 caaacggacg tggtggtggt cgccattatt ctttacgccc tgctcggcaa actggctgac
     3541 gtcagcgcgc agctgttaga gcgcctctgg ttgcgctgga acccggctta tcatttgaag
     3601 gaggccacgg tatgaatact gctcgtctga accagggcac accattattg ctcaatgcag
     3661 taagcaaaca ttacgcggaa aatatcgtcc tgaaccaact ggatttacat attccggcag
     3721 gtcagtttgt ggcggtggtg ggccgcagcg gtggtggcaa aagtaccctg ctgcgcctgc
     3781 tggcaggtct ggaaacgcca accgcaggcg atgtgttagc gggcaccaca ccgctggctg
     3841 agattcagga agacacgcga atgatgtttc aggatgcgcg tctgctgccg tggaaatcgg
     3901 tgattgataa cgttgggtta ggccttaaag gccagtggcg cgatgccgcg cgtcgggcgt
     3961 tggctgcagt aggactggag aatcgcgccg gggaatggcc tgccgcactt tctggcgggc
     4021 agaaacagcg tgtggcgctg gcaagagcgt tgattcatcg accgggttta ttgttgcttg
     4081 atgaaccgct cggggcactg gacgccttaa cgcgactcga gatgcaggat ttgattgtgt
     4141 cactttggca gcagcacggc tttaccgtac tgttggtgac gcatgatgtg agcgaagcgg
     4201 tagcaatggc tgaccgggtg ttgttaattg aagagggaaa aattggtctg gatttgacag
     4261 tggatatccc ccgtccgcgt cgattagggt cggtgcgatt agcggagctg gaagcggaag
     4321 tattgcaacg ggtaatgcgg cgtgggcatt ctgaacaact gattcggcgt catggttaat
     4381 atgccggatg tggcgcacaa aaccagcatt ttcaacggac ttgtaggcct gataagacac
     4441 gtcaagcatc gcatcaggca gttgggcacg gctgccggat gcggcgtgaa cgccttatcc
     4501 agcctacaaa accagcaatt tcaacggagt tgtaggcctg ataagacgcg tcaagcgtcg
     4561 catcaggcat tttggctcaa aagtatcaag ccagtgcttt ggtaatcttc tcgtacagat
     4621 cgccagagag attttccagc cctttcaact gttccagcgc cgcgcgcatt ttctcctgac
     4681 gtttggcatc ataacgtttc aggcgaatca gcggttcaat cagacgtgaa gctacctgcg
     4741 ggttacggct gttgaggtcg gtaagcattt ccaccaggaa ctggtaaccg ctgccatctt
     4801 cggcatggaa cgctgccggg ttgctgcccg caaacgcacc aatcaacgaa cgaatacggt
     4861 tcgggttgct catggtaaat gagcgatgct gcaacagacc acgcaccgtc tccagcacat
     4921 tcgccgccgg gctggtggct tgcaggataa accatttatc catcaccaga ccgtcctgat
     4981 gccacttatc gtcgtactcc tgcatcagcg cgtcacggca aggcagctgt gcagcaaccg
     5041 ccgcagaaag cgctgccagc gcatcggtca tattgtttgc ttcatggtat tgcttgctca
     5101 ccagcacgtc agccagatgc gtttcaccaa aagcaaggaa gcgcaggcag gcatttcgca
     5161 gagtgcgttt tgcaatatct tcatgctcaa cacggtactc gctctggtaa ttcgcgttgt
     5221 aaatagccag cagctcatcc gccagttcag tcgccagagt acgagtgagt gcttcgcgta
     5281 cttcggcaat agcaatcggg tcgatgatat cgaacagttc agccatttca ttgacagaag
     5341 gcagcgtcag gatttctgcc gccagcgccg gatcaatctt ctcatcgagc agcaccgcgc
     5401 ggaaagcgtc agccacatgt accggcagag acagcggctg cccttgctga tgacgggcga
     5461 cgttcagctt gatgtaggtt gccagcaaac tttgcgccgc atcccaacgg gagaaatcat
     5521 tacgcgcatg acgcatcagg aaggtcagtt gctgatcgct ccacttatat tccagtttca
     5581 ctggcgcaga gaattcgcac agcagcgcag gcaccggctg gaagtagaca ttatcaaaga
     5641 caaaggtctg ttccgcctga gtaacattca gcacggaatt caccggatga ccgcctttct
     5701 gcaatgggat cactttgcct tcgttatcat acagttcgat ggcaaacgga atatgcagcg
     5761 gctgtttttc tgcctgatcc ggcgtggctg gcgtgcgctg gctgatggtc agggtgtact
     5821 gctcggtttc cggattgtag tcgtctttga cggtcacaac tggcgtaccg gactggctgt
     5881 accaacggcg gaaatgggag agatcgacat tcgacgcatc ttccatcgcc tgaacgaaat
     5941 cgtcgcaggt cgctgcactg ccatcatggc gctcgaaata gagctgcatc cctttctgga
     6001 agttttcttc gccaagcaga gtgtggatca tacgaatcac ttccgctccc ttctcgtaaa
     6061 cggtcagggt gtagaagttg ttcatctcaa tgaccatgtc cgggcggatt gggtgcgcca
     6121 tcgggctggc gtcttccgca aactgcaatc cgcgcatggt gcgcacgttg ttgatacggt
     6181 ttaccgcgcg ggaaccaagg tcagagctga attcctgatc gcggaacacg gttaaacctt
     6241 ctttcaggct gagctggaac cagtcgcggc aggtcacacg gttaccggtc cagttatgga
     6301 aatattcatg gccgataacg cgttcaatat cgaggtaatc tttgtcggtg gcggtgtcgg
     6361 tgcgggccag cacatatttg gagttaaaga tattcagacc cttattctcc attgcgccca
     6421 tattgaagaa atccaccgcg acgatcatat agatgtcgag gtcatactcc aggccgaagc
     6481 gttcttcatc ccacttcatg gaatttttca gcgaggtcat cgcccacggt gcgcggtcga
     6541 ggttgccgcg atcgacatac agctccagcg ccacttcgcg accagaacgc gtggtgaagg
     6601 tatcgcgcag tacatcaaag tcgcctgcca ccagcgcaaa caggtagcac ggtttcggga
     6661 acgggtcctg ccattgcacc cagtgacgtc cgttttccag ttcgccttgc gcaacgcggt
     6721 tgccgttgga aagcaggaac gggtatttgg ttttatcggc aataattttg gtggtaaaac
     6781 gcgccagtac atccgggcgg tcgagataat acgtaatatg gcggaaacct tcggcttcac
     6841 actgggtgca aagcgcatcg cctgactgat aaagcccttc cagcgccgta ttcgccgccg
     6901 ggcttatttc attaatgatc ttaagcgtaa aacgctccgg caaattactg atgaccagtg
     6961 cgccctcttc ttctttccag gcggtccacg gctcatcatt aatatgaaca gaaaccagtt
     7021 tgaggtcttc gccgttgaga caaagcggag catctgatgc accatgacgg acagcctggc
     7081 tgaccgcggt aacgaccgtc ttttgcgcgt cgaggtcaaa ggtcaagtca atatcagtaa
     7141 tctggtaatc cggcgcacga taatcgtgac ggtatttggc ttgtggctgt tgagtcataa
     7201 ataaccttta gcatctttta tagagtctgg tgttcagtct attcctgttg cgtaaatctc
     7261 gctacgcaga atcttcatct tttcaggtac aaacgccttt attgctacat ttttataaca
     7321 tacaccgcgt aatgccatcg accagaaagg tggcatatgg tgtgatcggg gttcaataaa
     7381 ttgcgaaaca aggtatactc cagcagttcc tgaagatgtt tattgtacta aacgctcctg
     7441 tacgaggacg ctactgcgca cctatgacac aattcgcttc tcctgttctg cactcgttgc
     7501 tggatacaga tgcttataag ttgcatatgc agcaagccgt gtttcatcat tattacgatg
     7561 tgcatgtcgc ggcggagttt cgttgccgag gtgacgatct gctgggtatt tatgccgatg
     7621 ctattcgtga acaggttcag gcgatgcagc acctgcgcct gcaggatgat gaatatcagt
     7681 ggctttctgc cctgcctttc tttaaggccg actatcttaa ctggctacgc gagttccgct
     7741 ttaacccgga acaagtcacc gtgtccaacg ataatggcaa gctggatatt cgtttaagcg
     7801 gcccgtggcg tgaagtcatc ctctgggaag ttcctttgct ggcggttatc agtgaaatgg
     7861 tacatcgcta tcgctcaccg caggccgacg ttgcgcaagc cctcgacacg ctggaaagca
     7921 aattagtcga cttctcggcg ttaaccgccg gtcttgatat gtcgcgcttc catctgatgg
     7981 attttggcac ccgtcgccgt ttttctcgcg aagtacaaga aaccatcgtt aagcgtctgc
     8041 aacaggaatc ctggttcgtg ggcaccagca actacgatct ggcgcgtcgg ctttccctca
     8101 cgccgatggg aacacaggca cacgaatggt tccaggcaca tcagcaaatc agcccggatc
     8161 tagccaacag ccagcgagct gcacttgctg cctggctgga agagtatccc gaccaacttg
     8221 gcattgcatt aaccgactgc atcactatgg atgctttcct gcgtgatttc ggtgtcgagt
     8281 tcgccagtcg ctatcagggc ctgcgtcatg actctggcga cccggttgaa tggggtgaaa
     8341 aagccattgc acattataaa aagctgggaa ttgatccaca gagtaaaacg ctggttttct
     8401 ctgacaatct ggatttacgc aaagcggttg agctataccg ccacttctct tcccgcgtgc
     8461 aattaagttt tggtattggg actcgcctga cctgcgatat cccccaggta aaacccctga
     8521 atattgtgat taagttggta gagtgtaacg gtaaaccggt ggcgaaactt tctgacagcc
     8581 ctggcaaaac tatctgccac gataaagcgt ttgttcgggc gctgcgcaaa gcgttcgacc
     8641 ttccgcatat taaaaaagcc agttaatatc atcagggagc taatcggctc ccttttttta
     8701 cctttaattc cgaaatcttt cgctgcattt gcgaattctg cttgtctgat tgcagatgcc
     8761 aggtaacata ggtatccccc catttacggg tggacaagtg tttatttttt ccgactatta
     8821 acagagagaa tattatgagc gttgtgcctg tagccgacgt actccagggc cgcgtagccg
     8881 ttgacagcga agtcaccgta cgcggatggg tacgtacccg ccgagattca aaagctggca
     8941 tctccttcct cgccgtttat gacggttcct gctttgatcc tgtacaggct gtcatcaata
     9001 attctctgcc caattacaat gaagacgtcc tgcgtctgac caccggctgc tcggtcattg
     9061 tgacgggtaa agtcgtggcg tcgccgggcc aggggcaaca atttgaaatt caggccagca
     9121 aggttgaagt tgctggttgg gttgaagatc cagacactta cccgatggcg gcaaaacgcc
     9181 acagcattga gtatctgcgt gaagtcgctc acctgcgtcc gcgcacaaac ctgattggtg
     9241 ccgtcgcgcg cgttcgccat acgctggcgc aggcgctgca tcgcttcttt aacgagcagg
     9301 gattcttctg ggtttcaacg ccactgatta ctgcgtctga taccgaaggg gctggcgaaa
     9361 tgttccgcgt ttctacgctg gatctggaaa acctgccgcg taacgatcag ggtaaagtgg
     9421 atttcgacaa agacttcttt ggtaaagagt ctttcctgac cgtatctggc cagttgaacg
     9481 gcgaaaccta cgcttgcgca ttgtccaaaa tttatacctt cggcccgact ttccgtgctg
     9541 aaaactccaa caccagccgt cacctggcgg aattctggat gctggagccg gaagtggcgt
     9601 ttgctaacct gaacgatatc gcgggtctgg ctgaagccat gctgaaatat gtcttcaaag
     9661 cggttctcga agaacgcgca gacgacatga aattcttcgc tgaacgcgta gataaagatg
     9721 ccgtttcacg tctggaacgc ttcattgaag ccgattttgc gcaggtggat tacaccgacg
     9781 cagtgaccat tctcgaaaac tgcggcagga agtttgaaaa cccggtttac tggggcgttg
     9841 atctctcttc tgagcatgag cgttatctgg cggaagaaca ctttaaagca ccggtagtgg
     9901 ttaaaaacta tccgaaagat attaaagcgt tctatatgcg ccttaacgaa gacggtaaaa
     9961 ccgttgcggc tatggacgtt ctggctccgg gcatcggtga gatcattggt ggctcccagc
    10021 gtgaagaacg tctggacgtg ctggacgagc gtatgctgga aatgggcctg aataaagaag
    10081 attactggtg gtatcgcgat ctgcgtcgct acggtactgt tccgcattcc ggtttcggtc
    10141 ttggttttga acgcctgatt gcttacgtaa ctggtgtgca aaacgtgcgt gatgtgattc
    10201 cgttcccacg tactccgcgt aacgccagct tctaatttag cgtctacaag agccagcacc
    10261 cgctggctct tttttatcct ttctgtcaag ttatctgttt gttaagtcaa tcaatctatt
    10321 tgcaaccccg ccataacgat catcctgtta cggaatatta cattgcaaca tttacgcaca
    10381 aaaaataacc cgcattctta ttgcggatta gttttttctt agctgatagc acaattttca
    10441 tactattttt tggcgttctg gatgtctgaa agaggatttt gtgccaggtc gataaagttt
    10501 ccatcagaaa ca
//
LOCUS       DAPGOF010000173        10370 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.174, whole genome shotgun sequence.
ACCESSION   DAPGOF010000173 DAPGOF010000000
VERSION     DAPGOF010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.174"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            161..3943
                     /locus_tag="RHV13_003879"
     CDS             161..3943
                     /locus_tag="RHV13_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243357.1"
                     /translation="MGRKSREKRERKLTEQKVDYDSLIHDMSRYRELFTNGVGEDTYF
                     CKHLEATQNLFRRYKTLDVAVALFTSELWPANTGSPIKHIFAWCVLLELPFESQVGIS
                     IDSYVDFKVFTKELYASWPEFPMLEDFSPEADWGQIKVRLGQDFVPMFYGSCIERMSD
                     FVEAFRITYAHIPEAQAHMDLAIALQAQIIESIPELMLSVNTEAQCADVELATEDFWL
                     TCKSLLQQLGNELANRRKKAGCTFDTRVGTFKAPLKPDAFGNAVMQGMALPFLAVEMD
                     RVWIPMSVRSAPGLIIDHWANKNLNGISPSTHRKLAEFVAERFQRTLIGPLTLYVDGT
                     ACKDLPISCIILGNSGIYLICTCDHTSNDRLSNAAESIYAKVSRGSPIFFRLDDGRFL
                     SLSRVGAEPSVDELHIVIVVTLAGTAFNSINLPSKPTRLFPLADFITIFDSLNDLEEL
                     ESYWKFTDAQEGTLSPFSRGTVDLFASFKDIHGVLVEGAIAPNFIGLDPHWGTSWRFK
                     ILTEFWSRAPKVFPDSSIGWTLSSSTEGVVRLESRHHKAMAYSTSIGSCTVQTLIEIT
                     KELRFEDGRLIDLFAQLLADCSYRCRKMMSDISLFRQPHVLFTCSPEPSSLVKAEESP
                     SSLDEFKHVVTSVIPDSHNPDVFHVQVDTRAVLAGLNLSKDGSFEVRCLLETLEKCHA
                     VLGLQMPDEFAQRFTHIASQAARYHLKVMTRNIDVPDYVYPIIPSPTDYKLARRKLAA
                     EIMGLGFIPGRYELSDAKARIDPSSARMRLHIENRLAAFDRHQLLQAFIEQHDALLVT
                     ERTRIQRARLSLAHEVEYDRLDAIEHARKEFGDKARHYRYLLEKTVNLSSNGVDRVTE
                     DVLRELVGLVDWFMVLTGASDTLHNGIDVGGVEIDDSYIPEVFYSTDFGDREATFAKE
                     YAKSRLGLGTNDKDAVEGESEGLLASEKLKKAFITDLGFELQNMLTVLAVLSQAQRYG
                     FGNELSLSYAAAPDRLARGLADSIDRLDYSEAQKIVAFLTLSEVGVRRLVGRDIDEGD
                     IPYWERNKRIDRYTIKPLVIDGADLRWGAESASRAMYIWMSAVRDGYLPAEFDWPHVK
                     PVIREIKESIEKRLELRTEEIFRRHTPYVQRGIDFFRRFREEKFDDVGDFDVFAYWPE
                     SNSLVTVECKYNQPPFTMKDSRRLRDRIFGKAENDRAGQFSRILDRRKFLETHRSRLL
                     ELLQWPQPESVPLRNIELYVSRDVYYWMVHPPYPVPTQFVRVDTLDTWIKTELNIP"
     gene            3948..8375
                     /locus_tag="RHV13_003880"
     CDS             3948..8375
                     /locus_tag="RHV13_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDV1243358.1"
                     /translation="MSEDIKKTAITAAGYIYQNRQGLKVLCDWLDAPTRYSRVKFECD
                     NENEAPQGLDDIVIERSDGFVDLQQIKFTPDSETHLLSWDWMLEKTGKTERSRSMLRK
                     WFDAFKQLDPVRIGEISLITNRRPDAAIEVCLERDRIDPSKIPEPQRTLVEVELGGVE
                     ECKLFFRQLCIRHSDKGYLALEHEVDARLRLHGTPEGIANLKYVALNWATQKNLPAPD
                     GWIKLAEVGTILRASPPAPLPEDFVVPEGYEVPDEIFHQEFVQSTINATGKAIVLTGP
                     PGRGKSTYLSALCDTLAEKGIPTVRHHYFLSTTERGRDRVNSYIVEQSILAQIQQFHE
                     DVPKTGGGLHTQLEACAAHYKVLGKPFVLILDGLDHVWRINAEDKRPLDDLFCQVIPC
                     PENMVLLVGTQPVNDEQLPADLLAFAPKSEWHTLPAMSESAVLSYLRRTVQEGWLTTG
                     FESEEQIEEQLQSAASALRKKTNGHPLHVIYATSELKHSGRRLSSWDIEQLKGDLSQD
                     ARFYYASLWERLAPSQKDTLRLICAFTFFWPKTAFSEIATKIKAVEPDVDKVEHLLHS
                     SVAGLKVFHESLAVFVRATDDYESRINELMPVIADWLEHQAPTSLRVNWLWTVQAKLG
                     NPNNLITGLTRDWIMSRLKEGYSEALFEILLSEALSAALETANFSDAYRLAHLKARMV
                     DGSQFQMQDSDLARLISFTLTLTSEESVIRESIASRHETDILHVVALGLALLSRGDII
                     QAEICGEEAFHRFRALCQFSNKYNSRAGSDELLFLASAFTQLGVIADTSENLAWLVDK
                     NSPDIWLPRVQMLIQKGNLDDLMLLAASLPDGQKKNIISDACIRAAAVAGVVVTERED
                     FDELVRTPFVAAIEVALIKTSTPSSQPIPVNWISDSYYKHKDNLATLTHHWLFGAVHL
                     NLCMIAEGQTNFEHIKAPMYEDREDITDYLNVLSGIGAQIAQRWWCGEFINFHELYEL
                     LEPVESRRFRQSYFRKSATADFQAALHRIACDIYIGSVLLSGTSAVVLSQSTIDAALQ
                     CAWFDSASFRTQYASGSLIRMADEAAITFIGAQRLLLDADIQNETSVHLQIPLQLCAI
                     AISHQLNSEARELCTQTWELTTGYGHRKDPTLSNTVDAIAYLVDVVPKEACRLLAKIA
                     PQIHHVLDYTDGKGTRHVLAEADRLLAKLKPSALVIKFEEHTCVGDWALAESSMQAYI
                     KQGIIEEWPLDALMRTGLHPEIRDTLQQLVEDGCTTASERLRILEEHNGWDIGCLQRH
                     ENSSSDADNKPYEGDVTVFRPEQLEDLLKVLEPYYKERPKLLRTWYQHWESQKQGRNL
                     LVELDKILFAKNNKWMREALHLSEFAFQTKRRLSGLTAAWKYLVHAQIRNGGWIGYME
                     NVDVTCKRLDLVVQNYPQRCDEFVAVTTYNMFDEPVSSRLAPSEVMVYFYVKQNRIDE
                     AVKFTETMVNCVIEDTRTLPLEPPCWMSKLLEPIAQEVK"
     gene            8372..9820
                     /locus_tag="RHV13_003881"
     CDS             8372..9820
                     /locus_tag="RHV13_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug DMT transporter permease"
                     /protein_id="HDV1243359.1"
                     /translation="MNDLRILIARLGWPATSARWWTIQELAARLGEPETKAETESLLL
                     QLLRSRKLEAEVVEVLCIFWMAIKEFNYFPTTLLAENILLPSPLSSLLMKDFGLSILL
                     MNTDLELAPEDFIIPDDFDSVQGTDLPRIFHITISKLETFTRCPFIRQMAFEWTKNRT
                     AYPNAPYQGDAGYFIRPLGEGFAPHFSSRTALRVISAYLRTLAVARHIWRIPMELTEQ
                     HSLLALPIHPTLAFLRPYRPEWLSVSTEFKSDSKAIEASLRTFFAQVEAANPGDELIA
                     FSSPISMSMEQCVELSIVRWSQTVSSDKEGADSLTSHIESFWTREQMLSSQAPDPLST
                     TTLITPPTFEQLVNKTCMAWPLAGTLDFGRIGYLQHDLYPSRLFLPTMPGIENSLEVI
                     PHDGRLQVKLKDLVVAELYYWNAGWGPARLGQIQGNCGTALISRGTTYRERNITNTGS
                     LRSFYSWQVRRFHRNGSYGRFSEKITRGVVFV"
     gene            9919..>10370
                     /locus_tag="RHV13_003882"
     CDS             9919..>10370
                     /locus_tag="RHV13_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000077296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243360.1"
                     /translation="MSQFKFTELHAISEKPQNDDAAWLIEAERSIKFLKENAQNDEVV
                     IYAIGNSVLIHGALALEKNVTTADRWALQNMRMDVEYCWKIQKSWGGGQGHRIYLEPP
                     LDGVFKGGEALIYRRRFHGVEEGRAPIELSQKLVHSLGLYYLPERNAY"
BASE COUNT         2810 a         2181 c         2499 g         2880 t
ORIGIN      
        1 atgtaacaat ttgtcggatg tgtttgatag tttgaggcta tgcacttttg tgagggcggt
       61 acagtaagtg actcaagata ttcattatga tacgcattaa taatttctat gcttcgatac
      121 cgtatagact gtcaacgtat gcatacaggg aggatatgaa gtgggtagga agtcacgtga
      181 aaagcgggaa agaaaactaa cagagcaaaa ggttgattat gattctttga tacacgatat
      241 gtccagatat cgtgaattgt ttacgaatgg ggtaggtgaa gacacatact tttgtaagca
      301 tctggaagcc acccagaatt tatttcgccg atacaaaacc ctcgacgtag cagttgcatt
      361 atttacttcc gaactctggc cagccaatac aggtagcccc ataaagcata tttttgcttg
      421 gtgtgtcctt ttagagctgc catttgagtc tcaggtaggt atatcgattg atagctatgt
      481 agattttaaa gtatttacaa aagaattata cgcatcatgg cctgaattcc caatgcttga
      541 ggatttttcc cccgaggctg attgggggca aatcaaagta cggcttggtc aagacttcgt
      601 ccctatgttc tacgggagtt gcattgagag aatgtctgac ttcgtagaag catttcgcat
      661 tacctatgct catattccag aagcacaagc ccatatggat cttgccattg cgctgcaagc
      721 gcagatcatc gaatcgattc ctgagttgat gctctctgtc aacaccgagg cccaatgtgc
      781 tgatgttgaa ctagctactg aagacttctg gttgacttgc aagtctttgc ttcaacaact
      841 tggcaatgag ctagcgaacc gccgaaagaa agcagggtgt acgttcgata ctcgtgttgg
      901 cactttcaaa gcaccgctga aaccggatgc gttcggaaat gcagtgatgc aaggcatggc
      961 gctacctttc cttgctgtcg agatggatag ggtatggata ccaatgtcag tacgcagtgc
     1021 accgggtctc attattgacc attgggctaa caaaaatctg aacgggatta gcccctctac
     1081 gcacaggaaa cttgcagagt ttgtcgctga gcgattccaa agaaccctta tagggccttt
     1141 gactctgtat gttgacggca ccgcctgcaa agacctgcct atcagttgta ttatactggg
     1201 taattctggg atatatctca tttgcacatg tgaccataca tctaatgacc gcctgtcaaa
     1261 cgcagcggaa agcatatacg ccaaggtgag tcgtggatcg ccgattttct tccgtcttga
     1321 tgatggacgt ttcctttctc tatctagagt tggtgcagaa cctagcgtcg atgagcttca
     1381 catcgttatt gtcgttacac tcgcgggtac tgcgtttaat tccatcaatc tcccctcaaa
     1441 gccaactcgt ttgtttccat tagcggattt catcacgatt tttgatagtc tcaacgatct
     1501 ggaagaatta gaaagttatt ggaaatttac agatgctcaa gaggggacgc tcagcccatt
     1561 ttcccgtgga acagtagacc tgtttgcatc attcaaagac attcatggtg tgcttgtcga
     1621 aggtgcgatt gcacctaact tcattgggct agaccctcac tgggggactt cttggcgctt
     1681 caagatattg acagaattct ggtctcgtgc acccaaggta tttccagaca gctctatcgg
     1741 atggacgttg agttcttcaa ctgaaggtgt cgttcgactt gaatcgcgtc atcacaaagc
     1801 gatggcatac tcaacatcga ttggttcctg taccgttcaa acattaatag aaataaccaa
     1861 agaattacgc tttgaagatg gacgtctgat agaccttttt gcccaacttc tggccgactg
     1921 tagctatcgc tgcagaaaaa tgatgtctga tatttctcta tttaggcagc cgcatgtttt
     1981 gtttacgtgc tcccctgaac catctagtct ggttaaagct gaagagagtc cttcttcttt
     2041 agacgaattt aaacacgtgg tgacttcagt catcccggat tcccacaacc ccgatgtatt
     2101 tcacgttcaa gttgatacac gtgcagtgct tgctggatta aatctttcca aggatggatc
     2161 attcgaggtt cgttgcttac tggaaacgct cgaaaaatgt catgcagtac ttggtttgca
     2221 gatgcctgat gaatttgctc aacgatttac tcatatagcg tcgcaagcgg ctcgttatca
     2281 tttaaaagtc atgacccgta atattgatgt ccccgactat gtatacccta taattccttc
     2341 gcctaccgat tacaagcttg cccgtagaaa actggccgca gaaatcatgg ggcttgggtt
     2401 tattcctggc aggtatgaac tgtcagatgc caaggccagg attgatcctt ccagtgctcg
     2461 catgcgtctt cacattgaga atcgacttgc agcatttgat aggcatcagc ttctgcaggc
     2521 attcatcgag caacacgatg ccctccttgt tactgagagg actagaatcc aacgagcccg
     2581 tttgagtctt gctcatgaag ttgagtatga cagacttgat gcgatagaac atgcacgcaa
     2641 agaattcggt gataaggccc gccattatcg atatctacta gagaagaccg tcaatttatc
     2701 ttcaaatggt gtagatagag ttacagagga tgtactacga gaattagttg gcttggttga
     2761 ttggttcatg gtgctgactg gtgccagtga tacccttcac aatggtatag atgtgggtgg
     2821 tgttgagatc gacgactcat atattccaga agttttctat tcgacagatt ttggtgatcg
     2881 tgaggctacc tttgcaaagg agtatgcgaa gtccaggttg gggcttggca caaatgacaa
     2941 ggatgcggtg gaaggagagt cagaggggtt gctggcttca gagaaactca aaaaagcttt
     3001 tatcaccgat ttggggttcg agcttcagaa tatgctcact gttctcgctg tgttatcgca
     3061 agctcaacga tatggattcg ggaacgaact ttccttgtct tatgctgcag cacctgatcg
     3121 ccttgcgcga gggttggctg atagcattga taggcttgac tattctgagg cccaaaagat
     3181 tgttgctttt ctgactcttt cggaagtagg agttcgtagg ctcgttggaa gggatatcga
     3241 tgaaggagat attccatatt gggagcggaa taagcgaatc gatcgataca ctatcaagcc
     3301 gctggtaatt gatggtgccg atttgcgttg gggggcagag tcggcaagcc gggcaatgta
     3361 catttggatg tcggcggtac gagacggtta tttgcctgcg gaatttgatt ggcctcatgt
     3421 taagccagtc attcgcgaga ttaaggagag tattgaaaag cgtctcgagc ttcgtacaga
     3481 ggaaatattc cgcaggcata caccttatgt tcagcgaggt attgatttct ttagaagatt
     3541 ccgggaagag aagtttgatg acgttggtga tttcgatgtt tttgcatact ggccggaaag
     3601 caattcgctc gtgacagtag agtgtaagta caaccagccg ccatttacaa tgaaagatag
     3661 ccgtcgtctt cgtgatcgga tatttggcaa ggcagagaat gacagggcag gacaattcag
     3721 tcgtatttta gaccgcagaa aatttctcga aactcaccgt tcaaggttgc ttgaactgct
     3781 ccagtggcca cagccagaga gtgttccttt aaggaatatt gaactctatg taagccgcga
     3841 tgtgtactac tggatggttc atccgccata tcctgttccg acccagtttg tacgtgtaga
     3901 cactttggac acttggatca aaaccgaact aaacatacct tgatgctatg agtgaagata
     3961 ttaagaaaac cgccatcact gcagctggtt atatttatca aaatcgccag ggccttaagg
     4021 tcctgtgcga ttggcttgat gcgccgacgc gctatagccg cgtgaaattt gaatgtgata
     4081 atgaaaacga agcaccacag ggactagatg atatcgtcat cgaacgaagt gatggcttcg
     4141 ttgaccttca gcagatcaag ttcaccccag attctgagac gcatcttcta tcttgggatt
     4201 ggatgctgga gaaaacgggc aagacagagc ggtcgagatc gatgctacgc aaatggttcg
     4261 atgctttcaa acaacttgat ccagtgcgta ttggcgaaat ttcgctcata acgaatcgcc
     4321 gaccagatgc tgctattgag gtttgtctgg aacgagaccg gattgatcct tctaagatcc
     4381 cagaacctca gcgcaccttg gtagaagttg aactcggcgg cgttgaggaa tgcaaacttt
     4441 tcttcagaca attatgtatt cgacacagcg ataagggcta tcttgcattg gagcatgaag
     4501 ttgatgctcg ccttcgactg catggtacgc cagagggaat cgctaatcta aaatatgtgg
     4561 ctctgaattg ggcaacgcaa aaaaatctcc ctgcgccgga tggctggatt aaactggcag
     4621 aagttggaac catcttgcga gcttcacctc cagctccttt gccagaggat tttgttgttc
     4681 cagaaggtta tgaggttcct gatgaaatat tccatcaaga atttgtccag agcacaatta
     4741 atgctaccgg aaaggcaatt gtgctgactg gtccaccagg acgcggaaag agtacttacc
     4801 tgagcgcact gtgcgacacg cttgcagaaa aaggtatccc aacggttcgc catcactatt
     4861 ttctgtcgac aaccgagcga ggccgcgacc gggttaatag ctacattgtc gagcaatcca
     4921 ttctggcgca gatccaacag tttcacgagg atgtcccgaa aactggtgga ggcttacata
     4981 cacaattgga ggcatgtgct gcccactaca aagtactggg aaagccattt gtgctcattc
     5041 ttgatggttt ggatcatgtc tggcgtatca acgctgagga taaacgacca ctagatgacc
     5101 ttttctgtca ggtaattccc tgcccggaaa atatggtact gctcgtcggc actcaaccgg
     5161 taaatgacga acagttgcca gcggatttgc tcgcctttgc accaaaatct gagtggcata
     5221 cattgcctgc aatgtctgag agtgcagtac tttcttatct acgtagaaca gttcaagaag
     5281 gctggcttac aactggtttt gaaagtgaag aacaaattga agaacaactg cagagtgcag
     5341 catccgctct tcgtaagaaa actaatggcc atcctcttca cgttatttat gctacatcag
     5401 aattgaaaca ttcgggtcgc agactatcca gttgggatat tgagcagttg aagggagact
     5461 tgagccaaga cgcccgattt tactatgcat ctctatggga acgtcttgct ccaagccaaa
     5521 aggatacgct aaggcttatc tgtgccttta cttttttctg gcctaagaca gccttctcag
     5581 agattgctac caaaattaag gcggtggaac cagatgtgga taaagtagag catttgctcc
     5641 attcgtctgt ggctggtctt aaagtcttcc atgaaagttt ggctgttttc gtacgtgcaa
     5701 ctgatgacta tgagagtcga atcaatgaat taatgcctgt tattgcagac tggcttgaac
     5761 atcaggcacc tacttcacta cgagtgaact ggttatggac agttcaagcg aagcttggta
     5821 accccaacaa tctcattaca ggtttaactc gtgactggat catgagtcgc ttaaaagaag
     5881 gatattcaga agctttattt gagatattat tgtcagaagc cctgagcgct gcgcttgaaa
     5941 ctgcaaattt ttcagatgcc taccggttgg cgcatctgaa agctcgtatg gttgatggaa
     6001 gtcaatttca gatgcaggat agcgatctgg ctaggctcat atcttttaca ttgacactaa
     6061 cctcagagga aagtgtgata cgtgagtcta tagcctccag gcatgaaacg gatattttac
     6121 acgttgttgc acttggtctg gccttactgt ctcgagggga tattattcag gcagaaattt
     6181 gcggtgaaga agcctttcat cggttcagag ccctatgcca attttccaat aaatataatt
     6241 caagggctgg ttctgatgaa ctcttgtttc tggccagtgc atttacacag cttggcgtta
     6301 tcgctgatac atctgaaaat ctcgcttggt tagtcgacaa aaatagcccg gacatatggt
     6361 tacctcgcgt tcaaatgctg attcaaaaag gtaatttgga tgatttaatg ttgctagcag
     6421 cttctctacc cgatggacag aaaaagaaca taattagcga tgcttgtata cgagcggctg
     6481 cggttgcagg ggtagtagtg accgagcgag aggacttcga cgaacttgtc agaactccat
     6541 ttgtggctgc aatagaggtc gcgttaatca aaacctcaac tccttcgagc caaccaatcc
     6601 cggtgaactg gataagcgac agctattaca aacacaaaga taatttggca actctcacgc
     6661 accactggct gtttggtgcc gtacatctga atctttgtat gattgcagaa gggcaaacaa
     6721 acttcgagca catcaaagcc cctatgtacg aagatagaga agatattact gattatctca
     6781 atgtactgtc cggaattggt gcacaaatag cccaacgatg gtggtgtggg gaattcatca
     6841 acttccatga gctgtatgaa ttactcgagc ctgttgaatc gcggcgattt agacaaagtt
     6901 attttagaaa atcggcaacg gcggactttc aagcggcact acaccgtatt gcttgcgata
     6961 tatatattgg tagtgttctg ctttcgggaa ctagtgctgt ggtactttcc caatcgacta
     7021 ttgatgctgc gttgcaatgc gcttggttcg atagtgcgtc attccgcact cagtacgcat
     7081 ctggttcatt gatcagaatg gccgacgaag cggctataac attcattggc gctcaacgtt
     7141 tgttacttga tgcagacatt caaaatgaga ccagtgtaca tcttcaaata ccattacaac
     7201 tctgtgcgat agcgatctcc catcaactga attcggaggc tcgtgagctt tgcacgcaga
     7261 cgtgggaatt gaccactgga tacggacatc gtaaagatcc gacactgagt aatacggttg
     7321 atgccatcgc ttatttagtt gatgtagtgc cgaaagaagc ttgtcggtta ctggctaaaa
     7381 ttgcgccgca aatacaccat gttttggatt acaccgatgg taagggtacg agacatgttc
     7441 ttgctgaagc agatcggcta ctggcaaagc tgaagccttc cgcattggta ataaaatttg
     7501 aagagcatac atgtgtgggt gactgggctt tggccgagag cagtatgcag gcctacatca
     7561 aacagggaat cattgaagag tggcctctgg atgctctaat gcgcacaggc cttcatcccg
     7621 aaattcggga tacacttcaa caacttgtag aggatggttg tacaactgct tccgagcggc
     7681 ttcgcattct tgaagagcat aatggatggg atattggttg tttacaacgc cacgaaaatt
     7741 caagtagtga cgctgacaat aaaccttatg aaggtgatgt gacggtcttt agacctgaac
     7801 aattggaaga cttgctcaaa gttctggaac catattacaa agagcgtcca aaacttctcc
     7861 gtacttggta tcagcactgg gaaagccaaa agcaggggcg aaatttactc gtcgaactgg
     7921 ataagatttt atttgcaaaa aacaacaaat ggatgaggga agctctacat ctttccgagt
     7981 ttgcatttca aacaaaacgt agattaagtg gcttaactgc agcttggaag tatcttgttc
     8041 atgcacagat ccgtaatggg ggatggattg gttacatgga gaacgtggat gtgacttgca
     8101 aaagactcga cctggttgtc caaaattatc cgcagcgctg tgatgaattt gtggccgtaa
     8161 caacctacaa catgtttgat gagccagtgt cgtcacgcct agctccatcg gaagttatgg
     8221 tctatttcta cgttaagcaa aatcgcattg atgaagctgt aaagttcaca gagacgatgg
     8281 ttaattgtgt gattgaggat actcggacct tgcccttaga accaccttgc tggatgtcaa
     8341 aactgctcga gccaatcgcc caggaagtga aatgaacgat cttcgcattc ttattgcccg
     8401 tcttggatgg cctgctacgt ctgctcggtg gtggactatc caggagcttg ccgctcgact
     8461 gggagagcct gagacgaaag ctgaaaccga atcattgctg cttcaattgc tgcgctcgcg
     8521 caaacttgaa gccgaggttg tcgaagtatt atgtattttc tggatggcaa ttaaagagtt
     8581 taactacttt cctacgactt tgttggcaga gaatattctt ctaccctcac cactttcatc
     8641 attgttgatg aaagattttg ggttatcgat tctgctcatg aatactgatt tggaactagc
     8701 tccagaagac ttcatcattc ccgatgactt tgatagtgtt caaggtacgg atttgccaag
     8761 aatcttccat ataaccatta gcaagttgga aacttttacc cgctgtccat ttattcgaca
     8821 aatggccttc gaatggacaa aaaaccggac ggcatatcca aatgcaccgt accaagggga
     8881 tgcaggatat ttcattcggc ccttgggaga gggctttgcc cctcattttt cctctcgaac
     8941 agcccttcgc gtaatttccg cttatcttcg cacgttagct gtggctagac acatctggag
     9001 gataccaatg gagttgaccg agcaacattc tctcttggcg ttgcctatcc atccaacttt
     9061 ggcttttctt cgaccttatc gtcccgaatg gctctcagta tcgactgaat tcaaaagtga
     9121 tagtaaggcg atcgaggctt cattgcgaac tttctttgct caagtagagg cggcaaaccc
     9181 aggagacgag ctaattgcat ttagctcgcc catttcaatg tcaatggaac agtgcgtaga
     9241 gttgtcgatt gttcggtggt cacaaactgt aagcagtgat aaggaaggag cggactcgtt
     9301 gacatcccat attgaatcgt tctggacacg tgaacaaatg ctttccagcc aagctccgga
     9361 tccactcagt acaacaacct taataactcc accaactttt gaacaactgg tgaataaaac
     9421 ttgtatggct tggccattgg cgggaacgct cgattttggt cggataggat acctacaaca
     9481 tgatttgtat ccatcccggt tatttttacc gacgatgcca ggcatagaaa attctcttga
     9541 agtcatcccg catgatgggc gtctacaggt taagttaaag gacctagtcg tcgcagagct
     9601 ttattattgg aatgctggat gggggccagc taggctaggg caaattcaag gtaattgtgg
     9661 gacagccctc atatcgcgag gcactacata tcgcgagaga aacataacaa ataccgggtc
     9721 attgcgctca ttttactctt ggcaagtacg tagattccat cgtaatggaa gttatggcag
     9781 attcagtgaa aaaattacga gaggggtggt atttgtttga tgttatttct taattcataa
     9841 ggggaactta taacttataa cttataactt ataagtgatg tcagttatag ataaagtaaa
     9901 aggatatgaa tattactcat gagccaattt aaattcacag agcttcatgc tatttcggaa
     9961 aagccacaga atgatgatgc cgcttggtta atagaggctg aaaggtcaat taaatttctg
    10021 aaagaaaatg ctcaaaatga tgaagtggtt atctacgcga ttggaaatag tgtcctaatt
    10081 catggcgcgc tagctctaga gaaaaatgtt actacagcag atagatgggc attgcagaat
    10141 atgcgtatgg atgttgagta ttgttggaag attcaaaagt catggggtgg aggtcaagga
    10201 catcgtattt atcttgagcc acctttagat ggggtgttca aagggggaga ggcattaatt
    10261 tatcgtcgta ggtttcatgg cgttgaagaa ggccgtgcac ctatcgaatt aagtcagaaa
    10321 cttgtgcaca gccttgggtt atattacttg ccagaaagaa atgcatattg
//
LOCUS       DAPGOF010000174        10238 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.175, whole genome shotgun sequence.
ACCESSION   DAPGOF010000174 DAPGOF010000000
VERSION     DAPGOF010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10238
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.175"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            129..1523
                     /gene="galP"
                     /locus_tag="RHV13_003883"
     CDS             129..1523
                     /gene="galP"
                     /locus_tag="RHV13_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HDV1243361.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            1600..2097
                     /locus_tag="RHV13_003884"
     CDS             1600..2097
                     /locus_tag="RHV13_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HDV1243362.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MENVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            2192..2899
                     /gene="endA"
                     /locus_tag="RHV13_003885"
     CDS             2192..2899
                     /gene="endA"
                     /locus_tag="RHV13_003885"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HDV1243363.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSIGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            2979..3710
                     /gene="rsmE"
                     /locus_tag="RHV13_003886"
     CDS             2979..3710
                     /gene="rsmE"
                     /locus_tag="RHV13_003886"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDV1243364.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            3723..4673
                     /gene="gshB"
                     /locus_tag="RHV13_003887"
     CDS             3723..4673
                     /gene="gshB"
                     /locus_tag="RHV13_003887"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HDV1243365.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            4782..5345
                     /locus_tag="RHV13_003888"
     CDS             4782..5345
                     /locus_tag="RHV13_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HDV1243366.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            5345..5761
                     /gene="ruvX"
                     /locus_tag="RHV13_003889"
     CDS             5345..5761
                     /gene="ruvX"
                     /locus_tag="RHV13_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDV1243367.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNI
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVDVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(5924..6904)
                     /locus_tag="RHV13_003890"
     CDS             complement(5924..6904)
                     /locus_tag="RHV13_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HDV1243368.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLTKNQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGTPQILPELLKSENGLILVTGATGCGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVGEGRL"
     gene            6922..7626
                     /gene="yggS"
                     /locus_tag="RHV13_003891"
     CDS             6922..7626
                     /gene="yggS"
                     /locus_tag="RHV13_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HDV1243369.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            7644..8210
                     /gene="yggT"
                     /locus_tag="RHV13_003892"
     CDS             7644..8210
                     /gene="yggT"
                     /locus_tag="RHV13_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HDV1243370.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            8207..8497
                     /locus_tag="RHV13_003893"
     CDS             8207..8497
                     /locus_tag="RHV13_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HDV1243371.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            8505..9098
                     /locus_tag="RHV13_003894"
     CDS             8505..9098
                     /locus_tag="RHV13_003894"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HDV1243372.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDNQRQARFHCVLVYLRHAEDPTPLVCHGSWPGMITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            9091..10227
                     /gene="hemW"
                     /locus_tag="RHV13_003895"
     CDS             9091..10227
                     /gene="hemW"
                     /locus_tag="RHV13_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HDV1243373.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEDKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPSYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT         2406 a         2707 c         2706 g         2419 t
ORIGIN      
        1 gcacttaaat cataactaag ataaatgtta gtgtaagcga ttacactgat gtgatttgct
       61 tcacatcttt ttacgtcgta ctcacctatc ttaattcaca ataaaaaata accatattgg
      121 agggcatcat gcctgacgct aaaaaacagg ggcggtcaaa caaggcaatg acgtttttcg
      181 tctgcttcct tgccgctctg gcgggattac tctttggcct ggatatcggt gtaattgctg
      241 gcgcactgcc gtttattgca gatgaattcc agattacttc gcacacgcaa gaatgggtcg
      301 taagctccat gatgttcggt gcggcagtcg gtgcggtggg cagcggctgg ctctccttta
      361 aactcgggcg caaaaagagc ctgatgatcg gcgcgatttt gtttgttgcc ggttcgctgt
      421 tctctgcggc tgcgccaaac gttgaagtac tgattctttc ccgcgttctg ctggggctgg
      481 cggtgggtgt ggcctcttat accgcaccac tgtacctctc tgaaattgcg ccggaaaaaa
      541 ttcgcggcag tatgatctcg atgtatcagt tgatgatcac tatcgggatc ctcggtgctt
      601 atctttctga taccgccttc agctacaccg gtgcatggcg ctggatgctg ggtgtgatta
      661 tcatcccggc aattttgctg ctgattggtg tcttcttcct gccagacagc ccacgttggt
      721 ttgccgccaa acgccgtttt gttgatgccg aacgcgtgct gctacgcctg cgtgacacca
      781 gcgcggaagc gaaacgtgaa ctggatgaaa tccgtgaaag tttgcaggtt aaacagagtg
      841 gctgggcgct gtttaaagag aatagcaact tccgccgcgc ggtgttcctt ggcgtactgt
      901 tacaggtaat gcagcaattc accgggatga acgtcatcat gtattacgcg ccgaaaatct
      961 tcgaactggc gggttatacc aacaccaccg agcaaatgtg ggggacagtg attgtcggcc
     1021 tgaccaacgt acttgccacc tttatcgcaa tcggccttgt tgaccgctgg ggacgtaaac
     1081 caacgctaac gctgggcttc ctggtgatgg ctgctggtat gggcgtactc ggtacaatga
     1141 tgcatatcgg tatccactct ccgtcggcgc agtatttcgc catcgccatg ctgctgatgt
     1201 ttattgtcgg ttttgccatg agtgccggtc cgctgatttg ggtactgtgc tccgaaattc
     1261 agccgctgaa aggccgcgat tttggcatca cctgctccac cgccaccaac tggattgcca
     1321 acatgatcgt tggcgcaacg ttcctgacca tgctcaacac gctgggtaac gccaacacct
     1381 tctgggtgta cgcggctctg aacgtactgt ttatcctgct gacattgtgg ctggtaccgg
     1441 aaaccaaaca cgtttcgctg gaacatattg aacgtaatct gatgaaaggt cgtaaactgc
     1501 gcgaaatcgg cgctcacgat taatctcctc aggcttcctc ccgtcgcgga ggaagccgcc
     1561 tcttgcagtc atcttttctt cgctctatcc tctgccgcta tgaaaacatc ccgtctccct
     1621 atcgccatcc aacaggccgt tatgcgtcgc ctgcgggaaa aactcgccca ggctaacctg
     1681 aaattggggc gtaactaccc ggagccaaaa ctctcttata cccagcgcgg cacctccgcc
     1741 ggaacagcct ggctggaaag ctatgaaatc cgcctcaatc ccgttttgct gttggaaaac
     1801 agtgaagcct ttattgaaga agtggtgccg cacgaactgg cacatttgct ggtatggaaa
     1861 catttcgggc gcgtagcacc acatggcaaa gaatggaagt ggatgatgga aaacgtgctg
     1921 ggtgttcccg cccgtcgtac gcatcagttc gaactgcaat ccgtgcgtcg caacaccttc
     1981 ccctaccgct gcaagtgcca ggagcatcag cttaccgtac gccgccataa tcgcgtagtt
     2041 cgtggcgaag ccgtctatcg ctgtgttcac tgcggcgaac aactggtcgc aaaataacca
     2101 tctgaactat caggaacttt cctgatctgg ctgattgcat accaaaacaa ctttcgctac
     2161 gttgctggct cgttttaaca cggagtaagt gatgtaccgt tatttgtcta ttgctgcggt
     2221 ggtactgagc gcagcatttt ccggcccggc gttggccgaa ggtatcaata gtttttctca
     2281 ggcgaaagcc gcggcggtaa aagtccacgc tgacgcgccc ggtacgtttt attgcggatg
     2341 taaaattaac tggcagggca aaaaaggcgt tgttgatctg caatcgtgcg gctatcaggt
     2401 gcgcaaaaat gaaaaccgcg ccagccgcgt agagtgggaa cacgtcgttc ccgcctggca
     2461 attcggtcac cagcgccagt gctggcagga cggtggacgt aaaaactgcg ctaaagatcc
     2521 ggtctatcgc aagatggaaa gcgatatgca taacctgcaa ccgtcaatcg gcgaggtgaa
     2581 tggcgatcgc ggcaacttta tgtacagcca gtggaatggc ggcgaaggcc agtacggtca
     2641 atgcgccatg aaggtcgatt tcaaagaaaa agctgccgaa ccaccagcgc gtgcacgcgg
     2701 agccattgcg cgcacctact tctatatgcg tgaccaatac aacctgacac tctctcgcca
     2761 gcaaacgcag ctgttcaacg catggaacaa gatgtatccg gttaccgact gggagtgcga
     2821 gcgcgatgaa cgcatcgcga aggtacaggg caatcataac ccgtatgtgc aacgcgcttg
     2881 ccaggcgcga aagagctaac ctacactagc gggattcttt ttgttaaccc ctaccccacg
     2941 cgtacaaccg cgtggggaga cgacgcggat ttttaactat gcgtatcccc cgcatttatc
     3001 atcctgaacc actgaccagc cattctcaca tcgcgctttg cgaagatgcc gccaaccata
     3061 tcgggcgcgt actgcgcatg gggccggggc aggcgttgca attgtttgac ggtagcaacc
     3121 aggtctttga cgccgaaatt accagtgcca gcaaaaaaag cgtggaagtg aaggtgctgg
     3181 aaggccagat cgacgatcgc gaatctccgc tgcatattca cctcggtcag gtgatgtcgc
     3241 gtggtgaaaa aatggaattt acgatccaga aatcgatcga actcggtgta agcctcatta
     3301 cgccactttt ttctgagcgc tgcggcgtta aactggatag tgaacgtctg aacaagaagc
     3361 tccagcagtg gcagaagatt gcaattgctg cctgtgagca gtgtggtcgt aaccgggtgc
     3421 cggaaatccg tccagcgatg gatctggaag cctggtgtgc agagcaggat gaaggactga
     3481 aactgaatct tcacccgcgc gccagtaaca gcatcaatac gttgccttta ccggttgaac
     3541 gcgtccgcct gctgattggc ccggaaggcg gtttatcggc agatgaaatt gccatgactg
     3601 cccgctatca atttactgat atcctgttgg gacctcgcgt tttgcgtaca gagacaactg
     3661 cgctcaccgc cattaccgcg ctacaagtac gatttggcga tttgggctaa cggagaagaa
     3721 taatgatcaa gctcggcatc gtgatggacc ccatcgcaaa catcaacatc aagaaagatt
     3781 ccagtttcgc tatgttgctg gaagcacagc gtcgtggtta cgaacttcac tatatggaga
     3841 tgggcgatct gtatctgatc aatggcgaag cccgcgccca tacccgcacg ctgaacgtga
     3901 agcagaacta cgaagagtgg ttttcattcg tcggtgaaca ggatctgccg ctggccgatc
     3961 tcgatgtgat cctgatgcgt aaagacccgc cgtttgatac cgagtttatc tacgcgacct
     4021 atattctgga acgtgccgaa gagaaaggga cgctgatcgt taacaagccg cagagcctgc
     4081 gcgactgtaa cgagaaactg tttaccgcct ggttctctga cttaacgcca gaaacgctgg
     4141 ttacgcgcaa taaagcgcag ctaaaagcgt tctgggagaa acacagcgac atcattctta
     4201 agccgctgga cggtatgggc ggcgcgtcga ttttccgcgt gaaagaaggc gatccaaacc
     4261 tcggcgtgat tgccgaaacc ctgactgagc atggcactcg ctactgcatg gcgcaaaatt
     4321 acctgccagc cattaaagat ggcgacaaac gcgtgctggt ggtggatggc gagccggtac
     4381 cgtactgcct ggcgcgtatt ccgcaggggg gcgaaacccg tggcaatctg gctgccggtg
     4441 gtcgcggtga acctcgtccg ctgacggaaa gtgactggaa aatcgcccgt cagatcgggc
     4501 cgacgctgaa agaaaaaggg ctgatttttg ttggtctgga tatcatcggc gaccgtctga
     4561 ctgaaattaa cgtcaccagc ccaacctgta ttcgtgagat tgaagcagag tttccggtgt
     4621 cgatcaccgg aatgttaatg gatgccatcg aagcacgttt acagcagcag taacccacct
     4681 tagcgagaag gatctcgttg agactctgag tgacagcgcc cttctttcca cgcatactgg
     4741 gcgctgttgc ttttttgaac caggaaacag aacctctgac aatgaattta cagcatcact
     4801 ttcttattgc catgcctgct ctccaggatc cgattttccg tcgttccgtg gtctacattt
     4861 gcgaacataa taccaatggt gcaatgggga tcatcgtcaa caagccgctg gaaaatctca
     4921 aaattgaagg gattctggaa aagctgaaga tcacgccgga gccgcgtgat gaatcaatcc
     4981 gtctggataa accggttatg ctcggcggcc cactggctga agatcgcggg tttattttgc
     5041 atactccgcc ctccaatttt gcttccagca ttcgcatttc agacaacacg gtgatgacca
     5101 cttcccgcga tgtgctggaa acgctcggca ccgataaaca accgtctgac gtattggtgg
     5161 ctctgggtta tgcctcctgg gagaaaggtc aactggaaca agaaattctc gataacgcgt
     5221 ggctaacggc cccggcagat ctgaatattc tgttcaaaac gccgattgcc gaccgctggc
     5281 gcgaggcggc aaaactgatt ggtgtggata ttctcaccat gcctggcgtt gcggggcacg
     5341 cctgatgagt ggaaccttac tcgctttcga cttcggcacc aaaagcattg gcgtagcggt
     5401 cggccaacgc attaccggca ccgctcgccc tttgcctgca attaaagcac aggacggtac
     5461 gccggactgg aacattatcg agcgtttact gaaagagtgg cagccggacg aaatcatcgt
     5521 cggtttgccg ctgaatatgg acggtaccga acagccgtta accgccagag cgcgtaaatt
     5581 tgccaaccgt attcatggtc gtttcggtgt tgacgtaaag ctccatgacg agcgtcttag
     5641 caccgtggaa gcccgttccg gtctgtttga acagggcggc tatcgggcgc ttaacaaagg
     5701 caaagtcgac tctgcctctg cggttattat tctcgaaagc tatttcgagc agggatatta
     5761 aggcgattta aacgcctgct ggagtgtata atattatcgc gccttacgcc ttatccggcc
     5821 tacatgtggc agcgattgta ggtctgataa gacgcgcaag cgtcgcatca gacgtttatt
     5881 gcctttgtag gcccgataag cttgcacatc gggcatggca acgtcacaaa cgcccttccc
     5941 ccacccgctg ctgataactc tgctgaaacg ttatcatccc cacctgctgc ccggtttgaa
     6001 taacatgcgg taactggtgg gttttccctt cgcgaatcaa attccccacc gcgggtgtgt
     6061 taatcagcaa ttcaaatagc gccacgcgtc cttcctgttt atccacttcc agcttttgtg
     6121 acagcactgc ccgtaaacta cctgccagtt gattacgcac ggggtctttt tcctgcgccg
     6181 gaaatgaatc caccagtcgc tcaactgcct gcgcggcacc acgcgtatgt aatgttgcca
     6241 gcaccaaatg cccggtttct gccgccgtca gtgccagacg gattgtctcg ctgtcacgca
     6301 gctctccgag caaaatcaca tcaggatctt cccgcaatgc ggcccgcaat cccgatgcga
     6361 acgtcataca atgcaaaccg atttcccgct gctggatcaa acatcgctgg ctggcataga
     6421 gatattccac aggatcttcc agcgtcagaa tatgcgcatc agcatgttga ttgagataac
     6481 caaccatcgc cgccagcgtg gtagatttgc cacaccccgt cgcccccgtc accagaatca
     6541 agccattctc gctcttgagt aactccggca atatctgtgg tgtgccaagc tgttcgagct
     6601 gcgggcagtg cgaaggtaac agccgtaacg ccagagaaat accttgccgt tgcgcgaacg
     6661 cactgccacg caatcgctgg tttttcgtca gcgacacggc aaaatccagc tgaccattct
     6721 ccagcaatat tgcccgctga tcgtcatcca gccactcccg cagtagctct tcgacgtccg
     6781 gcgcatcaaa cggcgcagct tccattagcc cgcgaatgcg ccatcgtgcg ggccaggcgc
     6841 tgcacaggtg tagatccgag acgttatgct ttacactaag ggccacaatt tcttccatat
     6901 tcatactaag atcctcggaa aatgaacgat attgcgcata acctggcaca ggtccgggac
     6961 aaaatctcag cggctgcaac gcgttgcggc cgttctccag aagaaattac gctgcttgca
     7021 gtcagtaaaa caaaacctgc gagcgccatc gcagaagcca ttgatgccgg gcagcgtcaa
     7081 tttggtgaaa actacgttca ggaaggggta gataaaattc gccactttca ggaactgggc
     7141 gtaacaggat tagaatggca ttttattggc ccgttgcagt ctaataaaag ccgcctggtg
     7201 gcagagcatt tcgactggtg tcataccatc gaccgtttgc gcatcgctac ccgcctcaac
     7261 gatcagcgcc cggcagaact tccccctctt aacgtgctga ttcaaattaa tattagtgat
     7321 gaaaacagta agtccgggat tcaactggca gaactggacg agctggcagc agcggtcgct
     7381 gaactaccgc gtttacgtct gcgcgggctg atggcaatcc ctgcgcctga gtcagaatat
     7441 gtaaggcagt ttgaagttgc acgccaaatg gctgtagcat ttgccggact gaaaacgcgc
     7501 tacccgcata tcgacacgct ctctctggga atgtcggacg atatggaagc cgccattgcg
     7561 gcaggtagca cgatggttcg tatcggcact gcaatttttg gtgcgcgtga ttactctaaa
     7621 aaataaggaa ttaaaggaac gccatgaata cgttgacttt cctgctttca acggtcattg
     7681 agctgtatac catggtgctg ttattacgca tctggatgca gtgggctcat tgtgattttt
     7741 acaacccctt ctcacagttt gtagtgaagg taacacagcc aattatcggg ccactgcgcc
     7801 gcgttattcc ggcaatggga cccattgaca gcgcctcgct gctggttgcc tatattctca
     7861 gttttatcaa agccatcgtg ctgtttaaag tggtgacctt cctgccaatc atctggattg
     7921 ccggtttact gattctgctg aaaaccatcg gcctgctgat tttctgggtc ctgctggtga
     7981 tggcgattat gagctgggta agccagggtc gtagcccgat tgaatacgtg ctgattcagc
     8041 tagccgatcc gctgctgcgc ccgattcgcc gcctgctacc ggcaatgggt gggattgatt
     8101 tctcgccgat gatcctcgtt ctgctgctgt atgtcatcaa tatgggtgtc gcagaagtat
     8161 tacaggcaac cggaaacatg ctgctgccgg ggctgtggat ggcgttatga gtgccgtaac
     8221 agttaacgat gacggtctgg ttttacggct ttatattcag ccgaaagcca gccgtgattc
     8281 tattgtcggt ttacatggcg acgaggtaaa agtcgccata accgcgccgc cggttgacgg
     8341 ccaggccaac agccatctgg tgaagtttct cggtaagcaa ttccgggttg ccaaaagcca
     8401 ggtggtgatt gaaaaaggcg aacttggccg ccacaaacaa attaaaatca ttaatccgca
     8461 acaaatcccg ccagaaatcg cggcgttaat taattaggta tcctatgcaa aaagttgtcc
     8521 tcgcaaccgg caatgccggt aaagtgcgtg agctggcgtc gctgcttagc gacttcggtc
     8581 ttgatatcgt ggcccaaaca gacctcggcg tagattccgc tgaagaaacc ggcctgacct
     8641 ttatcgaaaa cgcgattctg aaagcgcgcc atgcggcaaa agtgaccggt ttaccggcaa
     8701 ttgccgacga ctctggtctg gcggtagatg tacttggcgg cgcgccgggc atttactccg
     8761 cgcgttattc cggtgaagac gcgaccgatc aaaagaatct gcaaaaactg ctggaaacac
     8821 taaaagacgt accggacaac caacgtcagg cgcgtttcca ctgcgtactg gtatatctgc
     8881 gtcacgcgga agatcccact ccgctggtgt gccacggcag ctggccgggc atgattactc
     8941 gtgaaccagc gggtactggc ggctttggtt atgatccaat cttcttcgta ccttccgaag
     9001 ggaaaaccgc tgccgaactg acccgcgaag aaaagagcgc catttcccac cgtggtcagg
     9061 cattgaaact gctgctggac gctttacgta atggttaaat taccgccgct gagtctctac
     9121 attcacatcc cgtggtgcgt gcagaaatgc ccgtactgcg atttcaactc tcacgcgttg
     9181 aaaggagaag tgccgcacga cgattacgtt cagcatctgc tttgcgatct ggacaacgat
     9241 gtggcttacg ctcagggccg tgaagtaaag acaatcttta ttggcggtgg tacgccgagc
     9301 ctgctttccg gcccggcgat gcaaacgctg ctggacggcg tgcgtgcgcg tttgccgctg
     9361 gcagcggatg cagaaattac tatggaagcg aaccctggta cggtagaagc cgatcgcttt
     9421 gtcgattatc agcgtgctgg tgtgaaccgc atctctattg gtgtgcagag ttttagcgaa
     9481 gacaagctga aacgacttgg gcgtattcat ggcccgcaag aagcgaaacg cgcggcaaag
     9541 ctggcgagcg ggctggggct gcgtagcttt aacctcgatt tgatgcatgg actaccggat
     9601 caatcactgg aagaggcgct tggcgatcta cgccaggcca ttgaactgaa tccgccgcat
     9661 ctttcctggt atcaactgac catcgaacct aatacgctgt ttggttcacg cccgccggta
     9721 ctgccggacg acgacgcgct gtgggatata ttcgaacagg ggcatcagtt attaaccgca
     9781 gcgggttatc agcaatatga aacttccgct tacgccaaac ccagttatca gtgccagcac
     9841 aatctcaact actggcgctt tggcgactac atcggtattg gctgcggcgc gcacggcaaa
     9901 gtgaccttcc cggatgggcg cattctgcgt accactaaaa cgcgtcatcc gcgtggtttt
     9961 atgcaaggaa ggtatctgga aagccagcgt gatgtcgaag ccgcagataa gccgtttgag
    10021 ttctttatga atcgcttccg tctgctggag gccgcgccgc gcgtggagtt tattgcgtat
    10081 accgggcttt gcgaagatgt gattcgccca cagttagacg aggcgattgc ccagggttat
    10141 ctcaccgaat gtgcggatta ctggcagata acggaacatg gtaaattatt cttaaactca
    10201 cttcttgaat tattcctcgc tgagtaatca ttaatgcg
//
LOCUS       DAPGOF010000175        10202 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.176, whole genome shotgun sequence.
ACCESSION   DAPGOF010000175 DAPGOF010000000
VERSION     DAPGOF010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.176"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            58..1740
                     /locus_tag="RHV13_003896"
     CDS             58..1740
                     /locus_tag="RHV13_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HDV1243374.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1792..2949
                     /locus_tag="RHV13_003897"
     CDS             1792..2949
                     /locus_tag="RHV13_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HDV1243375.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2915..3037)
                     /locus_tag="RHV13_003898"
     CDS             complement(2915..3037)
                     /locus_tag="RHV13_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243376.1"
                     /translation="MTTHPTGIYVGCFVKVIRRRLRLELRGRVINYSPFVLQHP"
     gene            3240..4925
                     /locus_tag="RHV13_003899"
     CDS             3240..4925
                     /locus_tag="RHV13_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HDV1243377.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVPP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKTDLLKEIICK
                     ALPQPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVL"
     gene            4963..7335
                     /locus_tag="RHV13_003900"
     CDS             4963..7335
                     /locus_tag="RHV13_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HDV1243378.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHVFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDTSSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSNVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSSTKTTITEYNNDGKTKTHSFNLSFELAEPLHISQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            7392..10175
                     /locus_tag="RHV13_003901"
     CDS             7392..10175
                     /locus_tag="RHV13_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDV1243379.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARGDNMQDAANALMAELATIDQHGFSTEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIAQFTA
                     VDALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
BASE COUNT         3005 a         2265 c         2241 g         2691 t
ORIGIN      
        1 cttatttctc ttcgtaaaat tacttcggaa ttaagtacaa taagaagagg aacatttatg
       61 aagtctgcat taaagaaaag tgtcgtaagt acctcgatat ctttgatact ggcatctggt
      121 atggctgcat ttgctgctca tgcggcagat gatgtaaagc tgaaagcaac caaaacaaac
      181 gttgctttct cagactttac gccgactgaa tacagtacca aaggaaagcc aaatattatc
      241 gtactaacca tggatgatct tggttatgga caacttcctt ttgataaggg atcttttgac
      301 ccaaaaacaa tggaaaatcg tgaagttgtc gatacctaca aaatagggat agataaagcc
      361 attgaagctg cacaaaaatc aacgccgacg ctcctttcat taatggatga aggcgtacgg
      421 tttactaacg gctatgtggc acacggtgtt tccggcccct cccgtgccgc aataatgacc
      481 ggtcgagctc ccgcccgctt tggtgtctat tccaataccg atgctcagga tggtattccg
      541 ctaacagaaa ctttcttgcc tgaattattc cagaatcatg gttattacac tgcagcagta
      601 ggtaaatggc acttgtcaaa aatcagtaat gtgccggtac cggaagataa acaaacgcgt
      661 gactatcatg acaacttcac cacattttct gcggaagaat ggcaacctca aaaccgtggc
      721 tttgattact ttatgggatt ccacgctgca ggaacggcat attacaactc cccttcactg
      781 ttcaaaaatc gtgaacgtgt ccccgcaaaa ggttatatca gcgatcagtt aaccgatgag
      841 gcaattggcg ttgttgatcg tgccaaaaca cttgaccagc catttatgct ttacctggct
      901 tataatgctc cgcacctgcc aaatgataat cctgcaccgg atcaatatca gaagcaattt
      961 aataccggta gtcaaacagc agataactac tacgcttccg tttattctgt tgatcagggt
     1021 gtaaaacgca ttctcgaaca actgaagaaa aacggacagt atgacaatac aattattctc
     1081 tttacctccg ataatggtgc ggttatcgat ggtcctctgc cgctgaacgg ggcgcaaaaa
     1141 ggctataaga gtcagaccta tcctggcggt actcacaccc caatgtttat gtggtggaaa
     1201 ggaaaacttc aacccggtaa ttatgacaag ctgatttccg caatggattt ctacccgaca
     1261 gctcttgatg cagccgatat cagcattcca aaagacctta agctggatgg cgtttccttg
     1321 ctgccctggt tgcaagataa gaaacaaggc gagccacata aaaatctgac ctggataacc
     1381 tcttattctc actggtttga cgaggaaaat attccattct gggataatta ccacaaattt
     1441 gttcgccatc agtcagacga ttacccgcat aaccccaaca ctgaggactt aagccaattc
     1501 tcttatacgg tgagaaataa cgattattcg cttgtctata cagtagaaaa caatcagtta
     1561 ggtctataca aactgacgga tctacagcaa aaagataacc ttgccgccgc caatccgcag
     1621 gtcgttaaag agatgcaagg cgtggtaaga gagtttatcg acagcagcca gccaccgctt
     1681 agcgaggtaa atcaggagaa gtttaacaat atcaagaaag cactaagcga agcgaaataa
     1741 ctaaaccttc atgcggcgga attttccgcc gccttattga gcgagatagc gatgcacgtt
     1801 acagccaagc cctccagttt tcaatgtaat ctcaaatgtg attactgttt ttaccttgaa
     1861 aaagagtcgc agtttactca tgaaaaatgg atggatgaca gcactctgaa agagttcatc
     1921 aaacaatata tcgcagcgtc tggcaatcag gtctatttta cctggcaagg cggtgaaccc
     1981 actctggctg gcctggattt tttccgtaaa gttattcact atcaacaacg ctatgcaggc
     2041 caaaaacgta tttttaatgc attacaaacg aatggcattt tattgaataa tgaatggtgt
     2101 gcctttctca aagaacatga atttctggtt ggtatctcga tcgatggccc ccaggagtta
     2161 catgaccgtt acagacgcag taattcaggt aacggtactt ttgcaaaagt gatagcagcc
     2221 atcgagcgtc tgaaatcata tcaagtagag tttaatacgt taaccgtcat taataacgtt
     2281 aatgtccatt accctcttga ggtttatcat tttttaaaat ctatcggcag taaacatatg
     2341 caatttatcg aattgctaga aaccgggacg ccgaatattg atttcagtgg tcatagtgag
     2401 aacacattcc gtatcattga tttttctgtg cctcccacgg cttatggcaa gtttatgtca
     2461 accattttta tgcaatgggt taaaaacgat gtgggtgaaa ttttcatccg tcagtttgaa
     2521 agctttgtca gccgtttttt ggggaatggg cataccagtt gtattttcca ggagtcctgc
     2581 aaggataatc tggttgttga aagtaatgga gatatttatg aatgcgatca ttttgtctat
     2641 ccacagtaca aaattggaaa cattaataaa tctgaactca aaacgatgaa cagtgtacaa
     2701 ctgacagcgc aaaaaaaacg gatttcagcg aaatgtcagc aatgtgcata taaacctatc
     2761 tgcaatggcg gttgtcctaa gcatcgtatt actaaagtaa acaatgagac tgtttcttat
     2821 ttttgcgaag gttataaaat ccttttttca accatggtac cttatatgaa cgccatggta
     2881 gagttagcta agaacagagt accgctttat cacattatgg atgttgcaaa acaaatggag
     2941 aataattaat aacacgccct cttagctcca gtcttagcct ccgtcttatg accttaacaa
     3001 agcacccgac ataaattcct gtcgggtgtg tcgtcatcat catcttccct tgcgaaaatc
     3061 ctggcatacg tggtatgcgt cagggcatta gcaataccct tgattatcaa tagcataacc
     3121 ataaccactg aaaattaaaa atttctatat atttcatatt gttaaatgac acttaaataa
     3181 atttcatcag taaatcattg ccatagataa tcattggatg gcacaatcaa ttttcgctaa
     3241 tgataaccat tcccattacg ttttgtatgt tgatagcaaa gtatctctgt ctgctgaagc
     3301 ccttttggct gcgtaagaat aataaaactt cagtgttgtt aatcattatt atcctcgcca
     3361 tgatccttgg cgtggttaaa attcaggtct ggttaaacga ctggaataat gattttttca
     3421 atgcgctaag ccagaaagaa accgataagc tttggcagct tgtcctttgg ttccctgccc
     3481 tgctggggat ctttgtatta atatccgtaa acaaaacatg gttaattaaa ctactgacta
     3541 ttcgctggcg cgaatggtta acagattact accttaatcg ctggtttgca gataaaaact
     3601 actacttcac gcagatctac ggtgagcata aaaatacaga taaccctgac caacgtatcg
     3661 ctgaagacat tctcttactg atcagcaaaa cgctcagcct ttcatttggc ttcatccagt
     3721 cacttagcat gctgatcacc tttaccgtta ttctttggca aagcgcgggt acgctctcat
     3781 tcactgttgg tggaacagaa tggaatatcc aggggtatat ggtctatacc gttgtgctca
     3841 ttgtgatcgg tggaacttta tttacccata aggtgggtaa acgtattcgt ccgcttaatg
     3901 tggaaaaaca acgtagcgaa gcgacatttc gaacaaatct tgtgcagcat aataagcagg
     3961 cagaactgat tgcgctaagc aatgctgagt ctctacaacg ccaggagttg agcgacaatt
     4021 ttcataccat taaagagaac tggcatcgtt taatgaatcg tcagcggtgg cttgattact
     4081 ggcagaatat ttattcgcgc tcgcttagcg ttcttcccta ctttctgtta ttgccgcagt
     4141 ttattagcgg gcagatcaat ctgggcggac tgatgaaatc gcgccaggca tttatgctgg
     4201 tatcgaacaa tttaagctgg tttatttata aatatgacga acttgctgaa ctggctgcgg
     4261 ttatcgatcg cttgtatgag ttccatcaac tcactgaaca gcgccctacg aataagccta
     4321 aaaattgcca acatgcggta caagtggctg acgcgagtat tcgtacccct gataataaga
     4381 tcatattaga gaacctgaac tttcatgttc cgccaggtaa atggctatta ctgaaaggtt
     4441 actctggtgc gggaaaaacc acactgctta aaacattatc ccactgctgg ccgtggttta
     4501 aaggtgatat ttcttctcct gctgacagtt ggtatgtgtc gcaaacaccg ttaatcaaaa
     4561 ccgacttact gaaagagatt atttgtaaag cacttcccca gcccgtagac gataaatcgt
     4621 tgagcgaagt actgcatcag gttggtcttg ggaaattggc tgcacgtatt catgaccacg
     4681 atcgctgggg agatattctt tccagcggcg aaaaacaacg tatcgccctg gcacgattaa
     4741 ttttacgacg tccgaaatgg atatttcttg acgaaactac ctctcatctt gaggaacaag
     4801 aggctatccg cttactgcgt ttagtgcgtg aaaaactacc cacaagcggc gtcattatgg
     4861 ttacacatca acccggtgtc tggaacctgg ctgatgatat ttgtgacatt agcgcggttt
     4921 tataaaaaca ataaaagccc tgctgtacgg agatataaat aaatgaagcg agttcttatt
     4981 cctggcgtca ttttatgtgg cgctgatgtg gcgcaggctg tcgatgacaa aaacatgtac
     5041 atgcatgttt ttgaagagat gacggtctat gctcctgtcc ctgtacccgt aaacggcaac
     5101 acgcattaca ccagtgaaag catcgagcgt ttaccgaccg ggaatggcaa tatcagcgat
     5161 ctgctgagaa ccaaccctgc ggtacgcatg gattcaacgc aaagtacctc gttgaaccag
     5221 ggagatattc gcccggagaa aatctctatt cacggtgcgt cgccctacca gaatgcctat
     5281 ttgattgatg gtattagtgc cactaataac ctgaacccag cgaatgagtc cgataccagt
     5341 agtgcaacca atattagcgg gatgtcacag gggtattatc ttgatgtcag cttactggac
     5401 aatgtgacgc tttatgacag ttttgtgccg gttgaattcg gtcgcttcaa tggcggggta
     5461 attgatgcaa agatcaaacg cttcaacgct gatgatagca acgtgaaatt gggttatcgc
     5521 actacgcgtt cggactggtt aacatcgcat atcgatgaga ataacaagag cgcatttaat
     5581 caaggttctt caggaagtac ttatttctct ccagatttca aaaagaactt ttataccttg
     5641 tcgtttaatc aggaactcgc tgataacttt ggcgttaccg ccggtttatc gcgccgccag
     5701 tctgatatca cccgcgcgga ttatgtttcg aatgacggca ttgtcgccgg tcgggcacag
     5761 tataaaaacg ttatcgatac cgcattgagc aaatttacct ggtttgccag cgaccgcttt
     5821 acccacgatt taaccttaaa atataccggc tccagtcgtg attataatac cagcaccttc
     5881 ccgcagtctg atcgcgaaat gggtaataaa tcctacggtc tggcatggga tatggatacg
     5941 cagctcgcat gggccaaact acgtaccacc gttggttggg atcatattag tgattatacc
     6001 cgtcacgatc atgacatctg gtacaccgaa ctttcatgta catatggtga tattacaggg
     6061 cgttgcaccc gtggcggatt aggacacatt tcccaggctg tagataatta caccttcaaa
     6121 acacgcctgg actggcaaaa attcgccgtg ggtaatgttt cgcatcaacc ctacttcggc
     6181 gcggaataca tctattccga tgcatggact gaacgccata accagtctga atcctatgtg
     6241 attaatgctg ccggaaagaa aactaaccat accatttacc ataaaggtaa aggcagcctg
     6301 ggaattgaca actacacact gtatatggcg gatcgcatta gctggcgtaa tgtgtcatta
     6361 atgcccggcg tgcggtatga ctatgacaac tatctgtcaa accacaatat ctccccgcgc
     6421 tttatgacgg aatgggatat ttttgctgat caaacctcaa tgattacagc aggttataac
     6481 cgttactatg gcgggaatat tcttgatatg ggattacgtg atatccgcaa tagctggacg
     6541 gaatcggtat caggtaataa aaccctgacg cgttatcagg atttgaaaac gccttataac
     6601 gatgaactgg caatgggatt gcagcaaaaa atcggtaaga acgttattgc gcgcgcaaac
     6661 tatgtttacc gtgaagcgca tgatcaaatc agcaaaagca gtcgtaccga cagctcgact
     6721 aaaaccacca ttactgaata taacaacgac ggcaaaacca aaacgcattc gttcaacctc
     6781 agttttgaac tggccgaacc cctgcatatc agccaggtag atattaaccc gcaaattgtc
     6841 tttagctata tcaagagcaa gggtaacttg tcgttaaaca atggttatga ggagagcaat
     6901 accggtgata accaggtggt ttataacggc aatctggttt cttacgatag cgttccagtg
     6961 gcggatttta ataacccatt aaagatctcc ttaaacatgg atttcacgca tcaaccgagc
     7021 gggttggtgt gggcgaatac gctggcctgg caagaagcgc gtaaagctcg cattatcctg
     7081 ggtaagacaa atgcgcaata catcagcgaa tattcagatt acaagcagta tgttgacgaa
     7141 aaactggata gcagcctgac ctgggacacc cgcttgtcct ggacgccaca atttctgaaa
     7201 caacaaaacc tgacgatcag tgccgatatt ctcaatgtac tggatagcaa aaccgcggtt
     7261 gatacaacga ataccggtgt ggcgacctac gccagtggcc gtactttctg gcttgatgtc
     7321 agcatgaaat tttaatagtt cggattttac ttaaccgggt aaattcgccc ggttttcgca
     7381 tggagataat tatgagaaac ctctgtttct tactgacgtt agtggcaact ctgttgctcc
     7441 ccgggcggct gattgccgcc gccttaccgc aggatgaaaa gttaattacc ggacaactgg
     7501 acaatggctt gcgatatatg atttatccgc atgctcaacc aaaggatcag gtaaatttat
     7561 ggctgcaaat tcataccggt tcattgcagg aagaagacaa tgagcgcggc gtggctcatt
     7621 ttgtagaaca tatgatgttt aacggcacaa aaacatggcc gggtaataaa gtcatcgaaa
     7681 catttgagtc aatgggcctg cgttttggtc gcgatgttaa tgcctatacc agctatgacg
     7741 aaacggtgta tcaggtgagt ttgccaacta cgcagaaaca aaatctgcaa caagtgatgg
     7801 caatcttcag tgaatggagt aatgccgcaa cctttgaaaa actcgaagta gacgctgaac
     7861 gcggcgtaat tactgaggaa tggcgtgccc atcaggatgc gaaatggcgc acctctcagg
     7921 cgcgccgccc tttcctgctg gcaaataccc gtaatttaga ccgtgaacct atcggcctga
     7981 tggatactgt cgccacggtc acaccggcac aattgcgcca attttatcaa cgctggtatc
     8041 aaccaaataa tatgaccttt atcgtggttg gcgatatcga cagtaaagaa gcgctggcgc
     8101 tgataaagga taatttaagt aagcttccgg ctaacaaagc agctgaaaat cgcgtctggc
     8161 cgacaaaagc cgaaaaccac ctgcgcttta atatcattaa tgataaagaa aaccgggtga
     8221 acggcatcgc actctattat cgcctgccaa tggtacaagt gaacgatgag caaagcttta
     8281 tcgaacaagc tgaatggagc atgttagttc agctgttcaa tcaacgtctg caggaacgca
     8341 tacagtcggg cgagttgaag actatttctg gcggcactgc gcgcagcgtt aaaattgcac
     8401 ccgattatca gtcgctgttt ttccgtgtaa atgcacgagg cgataatatg caggatgctg
     8461 cgaatgcatt aatggcagag ttggcaacca ttgatcagca tggtttttct actgaagaac
     8521 tcgatgatgt caaatctacc cgcctcacct ggctgaaaaa tgcggttgat cagcaagctg
     8581 aacgtgattt acgtatgctg accagtcgcc tggcatcaag ctcattaaat aatacgccgt
     8641 tcttgtcgcc ggaagagaca tatcaacttt cgaaacgtct gtggcagcaa attaccgtgc
     8701 aaagtctggc ggaaaaatgg cagcagttaa gaaagaacca ggacgcattt tgggagcaaa
     8761 tggtaaacaa tgagcttgcc gccaaaaaag cattgtctcc tgcggctatc ctggcgctgg
     8821 aaaaagagta cgccaacaaa aagctggcgg cttacatctt cccaggcaga aatttatcgt
     8881 taacagtaga cgctgaccca caggcggaaa ttagcagcaa agaaacgctg gctgagaatc
     8941 tgacatcatt aacactttcc aatggtgcca gggttattct ggcaaaatcc gcgggtgaag
     9001 agcaaaagct acaaattatt gccgtatcga ataaaggcga tttaagtttc cctgcgcagc
     9061 aaaaatcact tatcgcgctg gcaaataaag cagttagcgg aagcggcgtt ggcgaactct
     9121 cctcttccag cctgaaacgc tggagtgcgg aaaactcggt aaccatgagc agtaaagtca
     9181 gtggcatgaa tacgttgctc tccgttagcg cgcggactaa taaccctgaa cctggtttcc
     9241 agttgattaa ccagcgaatc acccacagca cgattaacga caatatttgg gcatcgctac
     9301 aaaatgctca aattcaggcg ttgaaaacgc tcgaccagcg tccagcggag aaattcgccc
     9361 agcagatgta tgagacgcgc tatgctgatg accgcacgaa attactgcaa gaaaatcaga
     9421 ttgcacagtt tactgccgta gatgcgctgg ctgccgatcg ccagttgttt tcatctccag
     9481 cggatatcac gtttgtcatt gtcggtaatg tcgcagaaga caaactcgtg gcgttaatta
     9541 cgcgttactt aggctcaatc aaacactctg attcgccatt agccgcaggt aaaccattaa
     9601 ctcgcgcgac ggacaacgca tcggttactg taaaagaaca aaatgaacct gtggcacagg
     9661 tttcacagtg gaagcgttat gattcccgga cacctgttaa tctggcgacg cgtatggcgc
     9721 tcgatgcttt taacgtcgca ctggcaaaag atctacgcgt taatattcgt gaacaggcat
     9781 ctggggcata cagcgtttct tctcgcctct cggttgatcc tcaggctaaa gatatcagtc
     9841 atttgctggc ttttacttgt caaccagaac gacatgatga actgttaacg ttagcgaatg
     9901 aagtgatggt taagcgtctg gctaaaggga tcagtgagca agaactgaat gaataccagc
     9961 aaaacgttca gcgcagcctc gatatccaac agcgtagcgt tcaacaatta gcgaacacta
    10021 ttgtaaatag tcttattcaa tatgacgatc ctgcagcatg gactgagcag gagcaattgt
    10081 tgaaacaaat gacggtagag aatgttaaca ctgccgttaa acaatatctt tctcatccgg
    10141 taaatactta tactggagta ttattgccaa aataataaca gccccgtcaa cacatcgttg
    10201 gc
//
LOCUS       DAPGOF010000176        10158 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.177, whole genome shotgun sequence.
ACCESSION   DAPGOF010000176 DAPGOF010000000
VERSION     DAPGOF010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10158
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.177"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(64..396)
                     /gene="emrE"
                     /locus_tag="RHV13_003902"
     CDS             complement(64..396)
                     /gene="emrE"
                     /locus_tag="RHV13_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HDV1243380.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSTPH"
     gene            complement(464..766)
                     /locus_tag="RHV13_003903"
     CDS             complement(464..766)
                     /locus_tag="RHV13_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HDV1243381.1"
                     /translation="MTGKEAIIHYLGTHKSFCAQDVAAVTGATVTSINQAAAKMARAG
                     ILVVDGKVWRTVYYRFATREEREGKVSTNLIFKECRQSAAMKRVLRVYKRTSMGTQ"
     gene            complement(763..1464)
                     /locus_tag="RHV13_003904"
     CDS             complement(763..1464)
                     /locus_tag="RHV13_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein P"
                     /protein_id="HDV1243382.1"
                     /translation="MKNIAAQMINFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVSAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVTAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELSCMTARINRGEAIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            complement(1461..2480)
                     /locus_tag="RHV13_003905"
     CDS             complement(1461..2480)
                     /locus_tag="RHV13_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDV1243383.1"
                     /translation="MTGSEMAKAGLLEQNRLSGANRNTLIAGGIMANTAEIFNFPVPD
                     AAQKEPRVADLDDGYTRIANELLEAVMLAGLTQHQLLVFLAVMRKTYGFNKKLDWVSN
                     EQLSELTGILPHKCSAAKSVLVKRGIFIQSGRNTGINNVVSEWSTLPESGKKNKVYLK
                     EVNLPESGKKSLPKSGKGVYPNQVNTKDKLTKDNIKPFSSENSGESSDQPENDLPVVK
                     PDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHR
                     DMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGVTASKPKLDLTNTDWI
                     YGVDL"
     gene            complement(2477..3016)
                     /locus_tag="RHV13_003906"
     CDS             complement(2477..3016)
                     /locus_tag="RHV13_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
                     /protein_id="HDV1243384.1"
                     /translation="MQPLTYQQTSGFSPTAVINRSQTKQVPGHEKIRDAVRAWSAVDN
                     QDVVATLIVNEYREQGGGTIDFPDDVSRARQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGYLVEQDSFMARLAEMEKELSEAKQAVILNAPRHQKLKEISEGIVSMFRV
                     DPDLAGPLMAMVTTMLGAI"
     gene            complement(3086..3316)
                     /locus_tag="RHV13_003907"
     CDS             complement(3086..3316)
                     /locus_tag="RHV13_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV1243385.1"
                     /translation="MNPAIKTAINIVGSQKKLGAACEVSQQAVYKWLHNKAKVSPEHV
                     GSIVTATGGVVKAYQIRPDLPKLFPHTEKNAA"
     gene            3421..4110
                     /locus_tag="RHV13_003908"
     CDS             3421..4110
                     /locus_tag="RHV13_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV1243386.1"
                     /translation="MKTTLSERLKEARLARGLTQKALGDLVGVSQAAIQKIETGKANQ
                     TTKIVEIANALGVRAEWLSSGVGNMSDSTVQPIQSTVSHSKYFKIDVLDIEVSAGPGV
                     INREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITVK
                     SFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKVI
                     GSMPQTYRKHG"
     gene            4706..4912
                     /locus_tag="RHV13_003909"
     CDS             4706..4912
                     /locus_tag="RHV13_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HDV1243387.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKHKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNGLNH"
     gene            4988..5284
                     /locus_tag="RHV13_003910"
     CDS             4988..5284
                     /locus_tag="RHV13_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HDV1243388.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIQYMVETIAHHQVDIDSEV"
     gene            5290..6075
                     /gene="bet"
                     /locus_tag="RHV13_003911"
     CDS             5290..6075
                     /gene="bet"
                     /locus_tag="RHV13_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HDV1243389.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIHASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            6072..6752
                     /locus_tag="RHV13_003912"
     CDS             6072..6752
                     /locus_tag="RHV13_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HDV1243390.1"
                     /translation="MIPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNITE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            6749..6931
                     /locus_tag="RHV13_003913"
     CDS             6749..6931
                     /locus_tag="RHV13_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HDV1243391.1"
                     /translation="MTHPHDNIRVGAITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEK
                     INNKREAVCTKHLLLS"
     gene            6904..7095
                     /locus_tag="RHV13_003914"
     CDS             6904..7095
                     /locus_tag="RHV13_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HDV1243392.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMVAKAEADERDQV"
     gene            7106..7387
                     /locus_tag="RHV13_003915"
     CDS             7106..7387
                     /locus_tag="RHV13_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243393.1"
                     /translation="MHFSGSGLHILCAYACRHGACSMTPQQENALRSIARQANSEIKK
                     ARQHFPDKNVDDICRSVLKKHRETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            7486..7695
                     /locus_tag="RHV13_003916"
     CDS             7486..7695
                     /locus_tag="RHV13_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HDV1243394.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNYQTVSATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEEIELKNKQ"
     gene            7752..8030
                     /locus_tag="RHV13_003917"
     CDS             7752..8030
                     /locus_tag="RHV13_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HDV1243395.1"
                     /translation="MFRIIFPNTWYVDHHGTPCKILRSTHNKVHYIRKGRTCIASMFR
                     FNHDFEPVNKADADRIAEEIETAEHIKKLRDMRSKSRGNHGIIQPHTR"
     gene            8229..9392
                     /locus_tag="RHV13_003918"
     CDS             8229..9392
                     /locus_tag="RHV13_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDV1243396.1"
                     /translation="MSLFRRNEIWYASYSLPGEKRIKESLGTKDKRQAQELHDKRKAE
                     LWRVEKLGDLPDVTFEEACLRWLEEKADKKSLDSDKSRIEFWLEHFEGIRLKDISEAK
                     IYSAVSRMHNRKTKEIWKQKVQAAIRKGKEPPVYEPKPVSTQTKAKHLAMIKAILRAA
                     ERDWKWLEKAPVIKIPAVRNKRVRWLEKEEAKRLIDECPEPLKSVVKFALATGLRKSN
                     IINLEWQQIDMQRRVAWVNPEESKSNRAIGVALNDTACKVLRDQIGKHHKWVFVHTKA
                     AKRADGTSTPAVRKMRIDSKTSWLSACRRAGIEDFRFHDLRHTWASWLIQSGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARKIDDIFGDNVPNMSHSGIMEDIKKA"
     gene            complement(9408..9484)
                     /locus_tag="RHV13_003919"
     tRNA            complement(9408..9484)
                     /locus_tag="RHV13_003919"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9448..9450),aa:Arg,seq:tct)
     gene            9727..>10158
                     /gene="fimZ"
                     /locus_tag="RHV13_003920"
     CDS             9727..>10158
                     /gene="fimZ"
                     /locus_tag="RHV13_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HDV1243397.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNS"
BASE COUNT         2770 a         2435 c         2407 g         2546 t
ORIGIN      
        1 ataaaacgag gctcatataa atataagagc ctccatattt tagtcgttta gaaacaaatt
       61 attttaatgt ggtgtgcttc gtgacaataa attaataacc aacacaccgg cacaaatcaa
      121 catcatgcct ataatggctg gcaggtccag ccgttggccg aaaaatcccc atgacagtaa
      181 gctaatcagg acaataccga ctcctgacca gatagcataa gcaatccctg taggaatata
      241 agccagcgtc tgagctaata accagaatga tgcacaataa caaataattg taccaacaga
      301 tggccataac cgtgtaaaac cttctgaaaa cttcattaag gttgtaccaa tgacctctgc
      361 aagtattgca ccaccaagat aaatataagg gttcatagca tattctttcc tgttcaaact
      421 ggagagaatt gtactacagt ttgaactcaa ctcacctgtt tcatcattgt gttcccattg
      481 atgttctttt atataccctc agtacccgtt tcatcgcggc actctggcga cactccttaa
      541 aaatcagatt cgtgctcacc tttccttccc gttcttctct ggtagcgaac cggtaataca
      601 ccgttcgcca gaccttacca tcaacgacca ggattcctgc ccgcgccatt ttagccgcag
      661 cctgatttat gctggttacg gttgcgcctg ttaccgcggc aacgtcctgt gcacagaagc
      721 tcttatgcgt ccccaggtaa tgaataattg cctctttgcc cgtcatacac ttgctccttt
      781 cagtccgaac ttagctttga tttctgcgat cttcgccaga gcctgtgcac gatttagagg
      841 tctaccgccc atgacaggaa gttgttttac tggttcaggt atcgcctcac cacggttaat
      901 tcgcgcggtc atacaggaca gttcatcggc agccttgcgc cgtaattccg cgtcagtcaa
      961 cgcattggcc cgcatgttct gatacaggtt ggtaaccagc cagtagtgcg cgtttgattt
     1021 ccacggataa gactctgcat ccggatacag accacgcttc cggcaatact cgtaaaccat
     1081 atcaaccagc tcgctgacgt ttggcagccc ggcggtaacg gatgcttctt cccggcacca
     1141 tgcaacaaac tgcccgggtg atggcagaaa tggtctattc tgccgacggg caacacgcat
     1201 tccggcgctc acctgttcca tcgtggtgat cccgttttcc cggaaagcca gaacccactg
     1261 gcggcggatt tcgttcagtt cgttctggtc acgattagcc aggctcgccg ggaaagttgc
     1321 cagtaactgg ctgaatacac cgttgattat ctgcgctacc tgctgtacct gcggcttttc
     1381 gtcgtactgt tccggcatgt tgttggcgat ccgacgcatc tgctcacggt caaagttaat
     1441 catctgtgcg gcgatgtttt tcatagatcc accccgtaaa tccagtctgt gtttgtcagg
     1501 tcgagttttg gtttgctagc tgtcacgcct gcctgttgct tgttacggtt gatttcgagt
     1561 tgggtccact tgtcgcggag tttggccgga cttagcacgt taccggacca gaagttgtcc
     1621 tggcatgccc agcggaacag cacgcacatg tcgcggtggt tacgtccgtc acgttcacgc
     1681 atcaggcgga tatcgttagc ccaccctgca aaattcggtt ttctggctga tggcgcgatg
     1741 gtcttcacca tgtcaaacat ccactctgcg gcggtcaggt cttctgctgt cccccacttg
     1801 ctgccgctct gaattgcagc atccggtttc accacaggaa gatcgttttc tggttggtca
     1861 gaggattcgc cagaattctc ggacgaaaaa ggttttatat tgtcttttgt tagtttgtct
     1921 tttgtgttta cctgattcgg gtaaacgcct ttacctgatt tgggtaaact ttttttacct
     1981 gattcaggta aatttacctc tttcaggtaa actttatttt tcttacctga ttcgggtaat
     2041 gttgaccatt cactgaccac attattaatg ccggtattcc gcccgctctg aataaaaatc
     2101 ccacgcttta ccagaacact ttttgcagca gaacacttgt gcggcaatat cccggttaat
     2161 tcggaaagtt gctcgttgct aacccaatcc agttttttat taaagccata tgttttgcgc
     2221 atgacagcca gaaagaccag aagctggtgc tgtgttaatc cggccagcat cacagcttcc
     2281 agcaactcat ttgcaatgcg cgtataacca tcatcgagat ctgccacgcg cggctccttt
     2341 tgtgccgcat ccggcactgg aaaattgaat atctcagcag tgtttgccat aattcctccc
     2401 gcaatgagtg tgttacgatt tgcacctgaa agtcggttct gttccagcag accggctttc
     2461 gccatttctg aacctgtcat atcgccccca gcatggtagt aaccatcgcc atcaatggac
     2521 cagccagatc tgggtccaca cgaaacatcg acacaatacc ttcactaatt tccttcagtt
     2581 tctggtggcg tggtgcgttg agaatgacag cctgttttgc ctcactgagt tccttttcca
     2641 tttcagccaa cctagccatg aagctatcct gctcaaccag gtaaccgcga tattccagcg
     2701 gtagtaccgc cagaattgcc ggggtcagtt cacgcacgtt atttcggtat ttttcagaat
     2761 cgaatttgtt atcgaggaag cggaacagct tctggcgtgc acggctgaca tcatcaggga
     2821 aatcgatggt gccgccgccc tgctcccgat actcattcac aatgagtgtg gcaacgacat
     2881 cctgattatc tacagccgac caggcgcgga cggcatcacg gattttttcg tggcctggca
     2941 cctgttttgt ttgagaacga tttatcaccg cagtcgggct aaatccgcta gtctgttggt
     3001 atgtaagtgg ttgcataatt gactccttta gtttgaattg actgttaagt tgattgctta
     3061 ttgttaaaga gcgtgaaatg gaaatttaag ctgcgttctt ttcggtgtgt ggaaacaact
     3121 tcggaagatc cgggcgaatc tggtatgcct tcactactcc accagtagcc gtaacaatgc
     3181 tgccgacatg ttcaggggat acctttgctt tgttgtgaag ccacttatag acggcctgct
     3241 gtgaaacttc gcaagcagcg cccagtttct tttgtgaacc aacgatattg atcgctgttt
     3301 tgatagctgg gttcataaca acctccgtgg ttaatttgaa tcaagattaa aactatggtt
     3361 gtttttagtc aacaaccatt ttcgtttgat ggaataaaac cttggttgta catttggact
     3421 atgaaaacaa cactctcaga aagacttaaa gaagccagat tagcgcgagg ccttacacaa
     3481 aaggcgcttg gggatttggt cggggttagc caggctgcta ttcagaaaat cgaaacaggg
     3541 aaagctaatc aaacaactaa aatcgtggag atcgcgaacg ctttgggtgt gcgcgcagaa
     3601 tggttatctt ctggcgttgg aaatatgtca gacagtacag tgcaaccaat acaatcaact
     3661 gtcagccatt ccaaatactt caagattgac gttcttgata tagaagtcag tgctgggccg
     3721 ggagtcatca accgtgagtt tgtagaagtt ctacgctcgg ttgagtactc gtttgacgat
     3781 gctcgtcaca tgttcgatgg taggaaggcg gaaaatatcc gcatcattaa cgtgcgtggt
     3841 gacagcatgt caggaacgat cgaaccaggt gatctgctgt tcgttgatat cacagttaaa
     3901 tctttcgacg gtgatggtat ctatgcgttt ctgtacgacg acacagccca tgtaaagcgc
     3961 ctgcaaatga tgaaggataa gctgctggtc atctctgata acaaaagcta ctcaccgtgg
     4021 gacccgatcg agaaagacga gatgaaccgg gtgttcatct tcggtaaggt tattgggagc
     4081 atgccgcaga catataggaa gcatgggtag taccaattaa aaattatcaa ctgggcattg
     4141 tgctcattca gtaaagaact aattcctatc tttgctctag gtagtaatat taagccaccg
     4201 caataatatc tttacctaac ggcgtaagaa tcccggtcac cgtgccgggt tttcttttgc
     4261 cctcccctca tcacacacac cgttaaaaaa accaccataa cctcgcttca gttatcgcta
     4321 tgcgattcaa gtcacaaaat aaatccatcc taaatacaac cagttatatc taaaacaacc
     4381 aataaaacaa cttttgttgt tgacgataaa acaactatag ttttaaataa gttcatcgca
     4441 acaacacaac gatacggcaa ctacctgatt caccgttgcg atgaccgctt agatccgcag
     4501 tttgaatttc agcaggcttc ggggagtgcg aggggtgaaa cggacgcgtg aacgtcggtg
     4561 tgaccagctg aaattaactc aacatttcat accttagtcg cttcaacgag gcggcttagt
     4621 tatgacaacc ggcggccatc caccgcctga atacgcgcag aagtctctat atgttcagca
     4681 gcccagctta cgggcaggag tttttatggt tcatcaacat tatggaacgc agaccgttaa
     4741 tcgaggtgcg gtcatgccag gaatgctggt caaacacaaa gatggtacct ggactgcatc
     4801 agctaattta cgcggacggc tttatctgca tcgcggcatc gagcgcactt atacccgtga
     4861 tttgctcgtg gaagtttttc tcgacggacg cggtaacggc ctgaatcact aatccccttt
     4921 cctgttttcc taatcagcct ggcatttcgc gggcgatatt ttcacagcca ttttcaggag
     4981 gtcagccatg aacgcttatt acattcagga tcgtcttgag gctcagagct gggcgcgtca
     5041 ctaccagcag atcgcccgtg aagagaaaga ggcagaactg gcagacgaca tggaaaaagg
     5101 cctgccccag cacctgtttg aatcgctatg catcgatcat ttgcaacgcc acggggccag
     5161 caaaaaagcc attacccgtg cgtttgatga cgatgttgag tttcaggagc gcatggcaga
     5221 acacatccag tacatggttg aaaccattgc tcaccaccag gttgatattg attcagaggt
     5281 ataaaacgga tgagtacagc actcgcaacg ctggcaggga agctggctga acgtgtcggc
     5341 atggattctg tcgacccaca ggaactgatc accactcttc gccagacggc atttaaaggt
     5401 gatgccagcg atgcgcagtt catcgcattg ctgatcgtcg ccaaccagta cggccttaat
     5461 ccgtggacga aagaaattta cgccttccct gataagcaga acggcattgt tccggtggtg
     5521 ggcgttgatg gctggtcccg catcatcaat gaaaaccagc agtttgatgg catggacttt
     5581 gagcaggaca atgaatcctg tacatgccgg atttaccgca aggaccgtaa tcatccgatc
     5641 tgcgttaccg aatggatgga tgaatgccgc cgcgaaccat tcaaaacccg cgaaggcaga
     5701 gaaatcacgg ggccgtggca gtcgcatccc aaacggatgt tacggcataa agccatgatt
     5761 cagtgtgccc gtctggcctt cggatttgct ggtatctatg acaaggatga agccgagcgc
     5821 attgtcgaaa atactgcata cactgcagaa cgtcagccag aacgcgacat cactccggtt
     5881 aacgatgaaa ccatgcagga gattaacact ctgctgatcg ccctggataa aacatgggat
     5941 gacgacttat tgccgctctg ttcccagata tttcgccgcg acattcacgc atcgtcagaa
     6001 ctgacacagg ccgaagcagt gaaagctctt ggattcctga aacagaaagc cactgagcag
     6061 aaggtggcag catgataccg gacattatcc tgcagcgtac cgggatcgac gtgagagctg
     6121 tcgaacaggg ggatgatgca tggcacaaat tacggctcgg cgtcatcacc gcttcagaag
     6181 ttcacaacgt gatagcaaag ccccgctcag gaaagaagtg gcctgacatg aaaatgtcct
     6241 acttccacac cctgctggct gaggtttgca ccggtgtggc tccggaagtt aatgctaagg
     6301 cgctggcctg gggaaaacag tacgagaacg acgccagaac cctgtttgaa ttcacttccg
     6361 gcgtgaatat tactgaatcc ccgatcatct atcgcgacga aagtatgcgc accgcctgct
     6421 cccccgatgg tttatgcagt gacggcaacg gccttgaact gaaatgcccg tttacctccc
     6481 gggatttcat gaaattccgg ctcggtggtt tcgaggcaat aaaatcggct tacatggccc
     6541 aggtgcagta cagcatgtgg gtgacgcgaa aagatgcctg gtactttgcc aactatgacc
     6601 cgcgcatgaa gcgtgaaggc ctgcattatg tcgtgattga gcggaatgaa aagtacatgg
     6661 cgagttttga cgagatggtg ccggagttca tcgaaaaaat ggacgaggca ctggctgaaa
     6721 ttggttttgt atttggggag caatggcgat gacgcatcct cacgataata tccgggtagg
     6781 cgcgatcact ttcgtctact ccgttacaaa gcgaggctgg gtatttcccg gcctttctgt
     6841 tatcagaaat ccactgaaag cacagcggct ggctgagaag ataaataata aacgggaggc
     6901 ggtatgcaca aagcatctcc tgttgagtta agaacgagta ttgagatggc acatagcctt
     6961 gctcaaattg gagtcaggtt tgtgccaata ccagtagaaa cagacgaaga atttcatacg
     7021 ttagccacat ccctttcaca aaagctggaa atgatggtgg cgaaagcaga agcagatgag
     7081 agagaccagg tatgacaacc actgaatgca tttttctggc agcgggcttc atattctgtg
     7141 tgcttatgct tgccgacatg gggcttgttc aatgacacct cagcaagaaa acgcccttcg
     7201 cagcattgcc cgtcaggcta attctgaaat caaaaaagcc agacagcatt ttccggataa
     7261 aaacgtcgat gacatttgcc gtagcgtact aaagaagcac cgcgaaacgg taacgctgat
     7321 gggattcaca ccgactcatt taagcctggc gatcggcatg ttgaacggcg tctttaagga
     7381 acggtaaaca tgaaaagcaa aatcatcagg gagctacagg ctcctttttt attattcgca
     7441 tttaccctca agcgtattaa ccaacaattc agggattaat gaaagatggc agacatcatt
     7501 gattcagcat cagaaatcga agaattacag cgcaatacag caataaaaat gcgtcgtctg
     7561 aactaccaga ctgtatccgc aactcattgt tgtgagtgtg gcgatccgat agatgagcga
     7621 agacgcctgg ctgttcaggg ttgtcggact tgtgcgagtt gccaggagga gatcgaactt
     7681 aagaacaaac aatagggact gtgatggcct caaagcagca aatttcaaca tcgtccaact
     7741 gaggtgtaaa aatgttcaga atcatttttc ctaacacctg gtacgtcgac caccacggca
     7801 ctccctgcaa aatcctgcgt tctacccaca acaaagttca ctacatccga aaaggcagaa
     7861 catgtatcgc cagcatgttc cgctttaatc atgactttga acctgtgaat aaagctgatg
     7921 cagatcggat agcagaagag atcgaaacgg cagaacacat taagaagtta cgtgacatgc
     7981 gttcaaaaag cagaggtaac catggaatca tacagcctca cactcgatga ggcctgtcag
     8041 tttcttaaga tatccagacc aaccgccacc aactggatac gaacaggccg cctacaggca
     8101 acacgtaaag atccaaccaa gccaaaatct ccttacctca caacacggca agcctgcatt
     8161 gcggcgcttc agtctccgct gcatactgtc caggtgagcg cgggtgatgg cataacagag
     8221 gaaagaaaat gtcactcttc cgcagaaatg aaatatggta tgcctcgtat tcgctcccgg
     8281 gcgagaaacg aattaaggaa tctcttggca caaaggacaa gcggcaagct caggagttgc
     8341 acgacaagcg aaaagcagaa ctctggcgag tagaaaagct aggggattta cctgatgtca
     8401 cttttgaaga ggcctgccta agatggcttg aggaaaaagc tgataaaaaa tctctcgatt
     8461 cagataaaag ccggattgag ttctggcttg aacattttga gggtataagg cttaaagata
     8521 tctcggaggc aaagatttac tctgctgtaa gcagaatgca taacagaaag acgaaagaaa
     8581 tatggaaaca gaaagttcag gccgccatca ggaaaggtaa agaaccgcct gtttatgaac
     8641 caaagccagt atcaactcag acaaaggcaa agcatcttgc catgataaag gccattctcc
     8701 gtgctgcaga acgcgactgg aagtggctgg aaaaagcgcc tgtcatcaag ataccagcgg
     8761 tcagaaacaa gcgagtcaga tggctggaaa aggaggaagc aaaacgcctt attgatgagt
     8821 gccccgaacc actgaaatct gtcgtcaagt ttgcgctggc aactggtctg agaaagtcga
     8881 acatcataaa tctggaatgg caacaaatcg acatgcagcg acgagttgcc tgggtgaatc
     8941 cagaagagag caaatcaaac cgcgccattg gtgtggcgct gaacgatacc gcctgtaaag
     9001 tgttgcgtga tcaaataggc aagcatcaca aatgggtgtt tgtacatacc aaggcggcta
     9061 agcgagcaga tggaacatca acgcctgcgg tcaggaagat gcgcatcgac agcaagacat
     9121 catggctatc agcttgtcgt cgtgcaggaa ttgaagattt ccgtttccat gacctcagac
     9181 acacctgggc aagctggctg attcagtcag gcgtcccatt atcagtgctt caggaaatgg
     9241 gcggatggga gtccatagaa atggttcgta ggtatgctca ccttgcgcct aatcatttga
     9301 cagagcatgc gaggaaaata gacgacattt ttggtgataa tgtcccaaat atgtcccact
     9361 ctggaattat ggaggatata aagaaggcgt aactgattga attgtaatgg cgcgccctgc
     9421 aggattcgaa cctgcggccc acgacttaga aggtcgttgc tctatccaac tgagctaagg
     9481 gcgcgttgat accgcaatgc ggtgtaatcg cgtgaattat acggtcaacc cttgctgagt
     9541 caatggcttt tgatctggtt gctgaacaag tgaacgaccg cgtctgattt tctgatttat
     9601 ttcgctatag cggcaaacaa acgcacaccg ctgcgcgtct gaatcaagaa aacccgtatt
     9661 ttcatgtatc aaagtacaat ttaccgccct aacggaaaat tgtccgctcc tatgagactg
     9721 gtaactatga aaccaacgtc ggtgatcatt atggatactc atcctatcat cagaatgtct
     9781 attgaagttc tgttgcaaaa aaacagtgaa ttgcagattg tcctgaaaac ggatgattac
     9841 cgcataacca tcgattatct ccgaacccgt cctgttgatt taatcattat ggatatagac
     9901 ttgcccggaa cagacggttt taccttcctg aaaaggatca aacaaatcca gagcacagtg
     9961 aaagtgttat ttttatcatc gaaatcagaa tgcttttatg ctggcagagc gatacaagct
    10021 ggtgctaacg gttttgtcag taaatgcaat gatcagaatg atatttttca tgccgttcag
    10081 atgatcctct ccggatacac gttttttccc agcgaaacgc ttaactatat aaaaagcaat
    10141 aaatgtagta cgaatagt
//
LOCUS       DAPGOF010000177        10146 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.178, whole genome shotgun sequence.
ACCESSION   DAPGOF010000177 DAPGOF010000000
VERSION     DAPGOF010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.178"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(10..492)
                     /gene="folK"
                     /locus_tag="RHV13_003921"
     CDS             complement(10..492)
                     /gene="folK"
                     /locus_tag="RHV13_003921"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HDV1243398.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILTVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPDGETLREILTSLAVLQPAIWY
                     "
     gene            complement(489..1907)
                     /gene="pcnB"
                     /locus_tag="RHV13_003922"
     CDS             complement(489..1907)
                     /gene="pcnB"
                     /locus_tag="RHV13_003922"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HDV1243399.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTA"
     gene            complement(1946..2872)
                     /gene="gluQRS"
                     /locus_tag="RHV13_003923"
     CDS             complement(1946..2872)
                     /gene="gluQRS"
                     /locus_tag="RHV13_003923"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HDV1243400.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARHGCWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQRLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGII
                     HADEKLAREDFIIYRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQVEAHWQDFS
                     VEQILQSAVKNWRLTAVPESAIVNSTFSNASC"
     gene            complement(2909..3364)
                     /gene="dksA"
                     /locus_tag="RHV13_003924"
     CDS             complement(2909..3364)
                     /gene="dksA"
                     /locus_tag="RHV13_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HDV1243401.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3542..4246)
                     /gene="sfsA"
                     /locus_tag="RHV13_003925"
     CDS             complement(3542..4246)
                     /gene="sfsA"
                     /locus_tag="RHV13_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HDV1243402.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4261..4791)
                     /gene="thpR"
                     /locus_tag="RHV13_003926"
     CDS             complement(4261..4791)
                     /gene="thpR"
                     /locus_tag="RHV13_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HDV1243403.1"
                     /translation="MSDPQRLFFAIDLPAEIREQIIHWRATHFPLEAGRPVAADNLHL
                     TLAFLGEVSPEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4865..7294
                     /gene="hrpB"
                     /locus_tag="RHV13_003927"
     CDS             4865..7294
                     /gene="hrpB"
                     /locus_tag="RHV13_003927"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HDV1243404.1"
                     /translation="MSSLPVVAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPTHQRFDDAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPAVNLLAAKRL
                     LKMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVRRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAGKWLPEYDWPAVDDESLLATLETWLLPHMAGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTLAQGRVPLVLELLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7490..10024
                     /gene="mrcB"
                     /locus_tag="RHV13_003928"
     CDS             7490..10024
                     /gene="mrcB"
                     /locus_tag="RHV13_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HDV1243405.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
BASE COUNT         2342 a         2603 c         2897 g         2304 t
ORIGIN      
        1 ataatacagc taataccaga tggcaggttg tagtactgct agcgaagtga gtatttcacg
       61 taatgtctcc ccatcaggaa acacgagttc cggggcaatc tcaaacagcg gccacagcat
      121 aaatccacga ttcttcatat cgtagtgcgg aacggtcagg cgttcggtat ttattacttc
      181 attaccaaac agcatgatgt cgagatccag cgtgcgcggt ccccagcgtt cagctttacg
      241 gacgcgacct tgctgcaatt caatacgctg tgtgtgattg agtagctctt caggtgcaag
      301 agaggtttcc agcgccacgg ctgcgtttaa gtaatcgggt tgatcttgcg gccccagcgg
      361 tggggtgcgg taaaacgaag aaacggtaag aatgtggctt tcagggatat cgcctaatgc
      421 tttcagggca gcattgacct gctccagcgg agaggccaga ttgctgccta tggcaatata
      481 cgccactgtc atgcggtacc ctcacgacgt ggtgcgcgtt tgcgtggacg acgagtacga
      541 cgacgcggtg atggttcttc atccagctcg ttgagcatcc ctttttggtc tggtggcgcg
      601 gaaacctgga actcacccca ccatttcacc agacgctgca gttcagcgtt acgctcaact
      661 tcagctcgca aggccaacag gtcataagcc gcacggaact taggatgctc cagcagtttc
      721 catgcgcgtt taccctgacg tcgggacata cgcaactgca actgccagat atcgcgggtt
      781 aatgtcgtca ggcgtttcgg gattgccagt gaacggcagg cttcgtccag cacgtcgttc
      841 atcgccagcg cgaaagcgtc gtgataggtc aggccgcttt cttgggcgat cttctgtgcc
      901 gtctccagca gtgggtacca gaacatggcg gcaaacagga acgccgggtt cacgcgcata
      961 tcgttatgga tacgcgtatc ggtattcttc agcacctgtt caatgatccg ctccatcggg
     1021 ctgtcgccat tttccgtgaa gtagcgggta atggtcggga acagcggctg gaacagatga
     1081 tattcacaca acagcttata ggtttcgtaa ccgtagcccg cttgtagcag tttaagcgat
     1141 tcttcaaaca ggcgtgccgg tgggatatcg ttcagcaggg tagcgaggcg agggatcggt
     1201 tctgcggttt ccgggctgat gcgcataccc aatttggcgg caaaacgtac cgcgcgcagc
     1261 atacgtaccg gatcttcacg gtagcgcgtt tccgggttac caatcagacg gataacgccg
     1321 tccttcagat ccttcatacc gccaacgtaa tcacggacgg taaaatccgc tacgctgtaa
     1381 tacaggctgt tgatagtgaa atcgcggcgc tgggcgtctt cttcgatgga gccgaaaatg
     1441 ttgtcgcgca gcaacatgcc gttttgcccg cgttgggagg tcgtgcggtc gctgacgtta
     1501 ccttcgtggt gtccacggaa ggttgcaact tcgataatct ccgggccaaa catcacatga
     1561 gccagacgga aacggcgacc caccaggcgg cagttacgga acagtttgcg cacctgctca
     1621 ggcgtggcgt tagtggttac gtcaaagtct ttcggctttt tgccaagtaa caggtcgcgc
     1681 acgccgccgc caaccagcca agcttcgtat cccgctttat tgagcctgta cattaccttc
     1741 agggcatttt cactgatatc tttgcgggaa atagcatgct gctcacgcgg gatcaccgtc
     1801 acctgtggac gggcgactgc ctgttcagcc tcgctttcct cgcggcttag caccttgcgg
     1861 caaaaattag cgactcgggt aaaaatagta cacctcggta gtgtcaaaca tcattcagga
     1921 caaaaaaata gcggctaatc atagctcagc atgacgcatt tgagaatgtt gaatttacaa
     1981 ttgccgactc aggcactgcg gtaagccgcc agtttttgac ggctgactga aggatttgct
     2041 cgacgctgaa atcctgccag tgtgcttcta cctgctgacc cagaaattga agtgccgcga
     2101 ttagtaccgg gcgtggatcg cctttcggca acgcaggcgc atgattctgc ttggaaagtt
     2161 tagcgccttg tggattaagc gccagcggca gatgaatgta atctggcact ttccagccaa
     2221 aaagctggta cagcgagatt tgccgcactg tcggttcgat caaatctgcc ccgcggacaa
     2281 tttcgctcac gccctggaag tgatcatcca ccaccaccgc caggttatag gcaaacaaac
     2341 catcacggcg ataaataata aaatcttccc gtgccagttt ttcgtcggcg tgaataatgc
     2401 cgcgcagctg gtcagtaaac tgggtgaccg gatgctgctg gcggatacgc actgcggcgt
     2461 tgtctggtcc atgatgcaac acccggcaat gaccgtcgta aataccgcca atgctttgaa
     2521 tacgcgcacg cgtgcaggtg cagtaataac ttagtctttg ttcatgtaac caggcgagtg
     2581 cttcacgata ggcatcgtga cgttgcgatt gccagagaac atcgccgtcc cagtgcagac
     2641 cgtaatgttc cagctggcgc aggatagttt ctgcggcacc gggaacttca cgaggcgggt
     2701 ctatatcttc aatgcgtacc agccagcaac cgtgccgggc gcgagcctgc aaatagctgc
     2761 caagcgcagc aatcagagaa ccaaaatgaa gctcgccgga aggagagggg gcgaagcggc
     2821 caatatactg tgtgtctgtc atctctttga acaaaaaata aggcgggagc atttcccgcc
     2881 tgtggtaaac gtgatggaac ggctgtaatt agccagccat ctgtttttcg cgaatttcag
     2941 ccagcgtttt gcagtcgatg cacagatcgg ctgtcgggcg cgcttccaga cggcgaatac
     3001 caatttcaac accgcaggat tcgcagtagc cgaaatcttc gtcttccact tttttcagcg
     3061 tcttctcgat ctttttaatc agcttacgct cgcgatcgcg gttacgcagt tcgaggctga
     3121 actcttcttc ctgggctgca cggtctaccg ggtccgggaa gttggctgct tcatcctgca
     3181 tatgtgtaac ggtgcgatcg acttcatccc tgagttgatt acgccatgct tccagaatac
     3241 gacggaagtg cgccagctgg gcttcattca tatactcttc gcccggcttc tcctgatatg
     3301 gttccacccc agcgatggcg agaatactca gggacgatgt tttacggttt tgcccttctt
     3361 gcatgttgct tctccttaac acgcactatc gatccccatg ttcgggggaa aaatgaggcc
     3421 gctataaata gcagatgctt ttccggatag caattatcta aacgtaacac ttgacaactg
     3481 tgtgaggaaa agcgtatttg cgcacgcgac cagaatgtaa attaaccagt tacttacttt
     3541 actacaatgt aaccggcagt gattttttaa gagccatgcc ttcagcagaa atttccgctt
     3601 tgtaagccag aatttctacc cccctctgtt gagcttctga caatagttgc gcgtatttct
     3661 catcgatgtg gcgcgcgggt gaaaaccgtg taatggctga atgcagcacg gcgaaaaaga
     3721 taaccgcacg ctggccttca gccgctacgc tcatcaactc ccgaaggtgt ttctgacctc
     3781 gttcagtgac cgcatcggga aaatatccct gttcgttctc cgctaacgta accgatttca
     3841 cttcaatata gcagtctgga cgcgaatccg cctgcaacat aaagtcaata cggctgcgtt
     3901 ctgcgccgta ttttacttcg cttttcagcg agctatagcc tgacagttct gaaattgatt
     3961 cattaaggat agcctctttc gtcaacctgt tagcccaaag cgtgttgacg caaataaatg
     4021 cgccgctctg gctttgagtt aattcccagg tgtgtgggta tttccgtttg gtgttgtctg
     4081 aagtcgaata ccagacggta tcgccaggcg ttgcacaacc ggtcatcgca cccgtattcg
     4141 ggcagtgtag cgttaattcg cgaccatcgg gtgtgatcac atcggctaaa aaacgtttgt
     4201 aacgctgaat tagcgtcgcg cgctgtagag ggggagaaaa ttccattgcg acaatccttg
     4261 ttattgcgtt agcgcccagc gttttagcgg cgtgtagcgt gtgcgtccac gggcaaacga
     4321 ggaggcgtaa agggtgaact ccgtcaccgc atacgaccag ttaaaacctg gcggcgggat
     4381 tgtcaccgcc tcgctggcgt cgcgcaataa ggtaatatgt ggatgaaacg gacgattgct
     4441 ttgaaaacaa ccgctgcggg cagcctgtga acggagcata ttcgccagct ggattaagcc
     4501 gcgtggaggc tgacgcatcc ctaaccacac cacacgcgaa cgcagccatt gtccggcgtc
     4561 atcaagcgtg agtgtgaaac caggttgacg aatccgtccg gctaaaagag aaagcgcctt
     4621 ctctttctcg gggctcactt cgcctaaaaa tgccagagtc agatgcaaat tatcggcagc
     4681 gaccggacgt ccagcctcaa gtgggaagtg tgtggcgcgc cagtggataa tctgttcgcg
     4741 gatttctgca ggtaagtcga tagcaaagaa cagacgttgc gggtcagaca tgtgaggcac
     4801 tcggttatga attaccgcga tgctacaatg tggcgcgaag aatgttaacc ctctggagcg
     4861 ttttgtgtcg tcgttgcccg ttgttgccgt cttacctgaa ttacttaccg ctctcgattg
     4921 tgcgccgcag gtattattaa gtgcgccgac cggggccggg aaatcaacct ggctgccgct
     4981 gcaactgctg gcgcatcccg gcattaacgg gaaaattatc ctgctggagc cgcgtcgtct
     5041 ggcggcgcgt aacgtcgcgc aacggctggc ggagctgctt aacgaaaagc caggcgatac
     5101 cgttggctac cggatgcgtg cgcaaaactg cgtcgggccg aatacccgcc tggaagtagt
     5161 taccgaaggc gtgctgacgc ggatgatcca gcgtgacccg gagttgagcg gtgttggatt
     5221 ggtgatcctt gatgaatttc atgagcgtag cttgcaggcg gatttggcgt tggcgctgtt
     5281 actcgatgtg caacaaggtc tgcgtgatga ccttaaactg ctgattatgt cggctacgct
     5341 ggacaacgac cgcttgcagc aaatgctgcc agaagcgccc gtcgtcatct cagaagggcg
     5401 ctcgtttccg gttgaacgcc gttatttacc gctgcccacg catcagcgtt ttgacgatgc
     5461 cgttgcggta gccaccgctg aaatgctgcg tcaggaaagt ggatcattac tgttattttt
     5521 acctggcgtc ggagaaattc agcgtgtgca ggaacaactg gcttcgcgca tcggcagcga
     5581 tgtattgctc tgcccgctgt atggcgcgtt gtcgctgaac gatcagcgaa aagcgatcct
     5641 cccggcaccg caagggatgc gcaaagtggt gctggcgacc aatattgctg aaaccagttt
     5701 aaccattgaa ggtattcgtc tggtggtgga ttgtgcccag gagcgtgtgg cgcgttttga
     5761 tccgcgcacg gggcttacgc gactgattac tcaacgcgtt agccaggcat ccatgacgca
     5821 gcgtgccggg cgcgccgggc gtctggagcc gggtatctgc ctgcatttaa tcgccaagga
     5881 acaagcagaa cgcgccgcgg cgcaaagtga accggaaata ttacaaagcg atctttccgg
     5941 tttgctgatg gaattactgc aatggggatg cagcgatccg gcgcagatga gctggctgga
     6001 tcaaccgcca gcagtgaatt tactggccgc gaaacgcctg ttaaagatgc tgggggcgct
     6061 ggacggtgaa cggcttagtg cgcaggggca aaaaatggca gcgctgggta acgatccgcg
     6121 tttagcggca atgctggtta gcgcaaagag cgacgacgaa gctgctaccg cggcaaaaat
     6181 tgccgccatt cttgaagagc cgccacggat gggcaatagt gacctgggcg tggcgttttc
     6241 gcgcaatcaa ccagcctggc agcaacgtag tcagcaactg ttaaaacgct taaacgtacg
     6301 cggcggtgag gcagacagtt cgcttatcgc gccgctactt gccggggcgt ttgccgatcg
     6361 cattgctcgc cgccgtgggc aagatggacg ctatcaactg gcaaacggca tgggagcgat
     6421 gctcgatgcc gacgacgcgc taagccgcca cgaatggttg atcgcaccgt tattattgca
     6481 gggcagcgcc tcgccggatg cgcggatttt actggcgcta ccggttgata ttgatgaatt
     6541 agtgcgacgc tgtccgcagc tggtgcagca atctgacacc gtggaatggg atgacgcgca
     6601 aggtacactg aaagcctggc gtcggctgca aatcggtcag ttgacggtga aagtgcagcc
     6661 gttggcgaaa ccgtcagaag acgagttgca tcaggcgatg cttaacggca tccgtgataa
     6721 aggtttaagc gtgctcaact ggacggcgga agcggaacag ctacgcttgc gtttgttatg
     6781 cgccggaaag tggttgccag aatatgactg gccagcggtt gatgatgaaa gtttgttggc
     6841 gacgctggaa acgtggctgc tgccacatat ggccggtgta cattcactac gtggcctgaa
     6901 atcactcgac atttatcagg cactacgcgg attacttgat tggggaatgc agcaacgtct
     6961 ggatagtgaa ttgcctgcgc attacactgt gccgacggga agccggatcg ccattcgtta
     7021 tcatgaagat aacccgcccg cgctggcggt gagaatgcag gagatgtttg gtgaggccac
     7081 taatccgacg ctcgcccagg ggcgcgtgcc gctggtgctg gaattgcttt cacccgccca
     7141 aaggccgctg caaatcacgc gtgatttggg ggccttctgg aaaggggcgt atcgtgaggt
     7201 gcaaaaggag atgaaagggc gttatcccaa acacgtctgg ccggacgacc cggcaaacac
     7261 tgcaccgacg cgacggacga aaaagtattc gtaaaacggg tatgagcagt cccctcgccc
     7321 ctatggggag agggataggg tgaggggtaa tgtgcgcgct gatgccctca ccccggccct
     7381 ctcccacagg gagagggaga aaagcaaatt ttgagagata tcttcttctg tcttgtaaca
     7441 gaagaacaga aaatcgggct tttgcgcctg aatattgcgg agaaaaagca tggccgggaa
     7501 tgaccgcgag ccaattggac gcaaagggaa accgacgcgt ccggtcaaac aaaaggtaag
     7561 ccgtcgtcgt tacgaagatg acgatgatta cgacgattat gatgactatg aggatgaaga
     7621 accgatgccg cgcaaaggta agggcaaagg caaagggcgt aagcctcgtg gcaaacgcgg
     7681 ctggctatgg ctgctgttaa aactggctat cgtttttgcc gtgctgatcg ccatttacgg
     7741 cgtttatctc gatcaaaaaa ttcgtagccg tattgatggc aaggtctggc aactgcctgc
     7801 ggcagtttat ggccgaatgg tcaaccttga gccagacatg accatcagca agaacgaaat
     7861 ggtgaagctg ctggaggcaa cccagtatcg tcaggtgtca aaaatgaccc gtcctggcga
     7921 atttaccgtg caggccaaca gcattgagat gattcgccgt ccgtttgatt tcccggacag
     7981 taaagaagga caggtgcgcg cgcgtctgac ctttgatggc gatcatctgg cgaccatcgt
     8041 caatatggag aacaaccgtc agttcggttt cttccgtctt gatccgcgcc tgatcaccat
     8101 gatctcctca ccaaacggtg agcaacgtct gtttgtgccg cgcagtggtt tcccggattt
     8161 gctggtggat actttgctgg cgacagaaga ccgccatttc tacgagcatg atggaatcag
     8221 tctctactcc atcggacgtg cggtgctggc aaacctgaca gccggacgca cggtgcaggg
     8281 ggcgagtacg ctgacgcaac agctggtgaa aaacctgttc ctctccagcg agcgctctta
     8341 ctggcgtaaa gcgaacgaag cttacatggc gctgatcatg gacgcgcgtt acagcaaaga
     8401 ccgtattctt gagctgtata tgaacgaggt gtatctcggt cagagcggcg acaacgaaat
     8461 ccgcggcttc ccgctggcga gcttgtatta ctttggtcgc ccggtagaag aattgagcct
     8521 cgatcagcag gcgctgttag tcggtatggt gaaaggagcg tccatctaca acccgtggcg
     8581 taacccgaaa ctggcgctgg agcgacgtaa tctggtactg cgtctgctgc aacagcaaca
     8641 gattattgat caagaactct atgacatgtt gagtgcgcgt ccgctgggtg ttcagccgcg
     8701 cggtggggtg atctctcctc agccagcctt tatgcaactg gtgcgtcagg agcttcaggc
     8761 taaattaggc gataaggtaa aagatctctc cggcgtgaag atcttcacaa cctttgactc
     8821 ggtggcccag gacgcggcag aaaaagccgc cgtggaaggc attccggcac ttaagaaaca
     8881 gcgtaagctg agcgatctgg aaaccgcgat tgtggtcgtc gaccgcttta gtggtgaagt
     8941 tcgtgcgatg gtcggaggtt ctgagccgca gtttgcgggc tacaaccgtg cgatgcaggc
     9001 gcggcgttcg attggttccc ttgcaaaacc agcgacttat ctgacggctt taagccagcc
     9061 gaaaatctat cgtctgaata cgtggattgc ggatgcgcca atcgcgctgc gtcagccgaa
     9121 tggccaggtc tggtcaccgc agaacgatga ccgtcgttat agcgaaagcg gcagagtgat
     9181 gctggtggat gcgttgaccc gttcgatgaa cgtgccgacg gtaaatctgg ggatggcgct
     9241 ggggctgcct gcggttacgg atacctggat taaactgggc gtgccgaaag atcagctgca
     9301 tccggttccg gcgatgctgc tgggggcgtt gaacttaacg ccaatcgaag tggcgcaggc
     9361 attccagacc atcgccagcg gcggtaaccg tgcaccgctt tctgcgctgc gttcggtaat
     9421 cgcggaagat ggcaaagtgc tgtatcagag cttcccgcag gcggaacgcg ctgttccggc
     9481 gcaggcggcg tatctgacac tatggaccat gcagcaggtg gtacaacgtg gtacgggtcg
     9541 tcagcttggg gcgaaatacc cgaacctgca tctggcaggg aaaacaggga ctaccaacaa
     9601 taacgtagat acatggtttg cgggcattga cggcagcacg gtgaccatca cctgggtagg
     9661 ccgtgataac aaccagccga ccaaactgta tggagccagc ggggcaatgt cgatttatca
     9721 gcgttatctg gctaaccaga cgccaacacc gctgaatctt gttccgccag aagatattgc
     9781 agatatgggc gtggactacg acggcaactt tgtttgcagc ggtggcatgc gtgtcttgcc
     9841 ggtctggacc agcgatccgc aatcgctgtg ccagcagagt gagatgcagc agcagccgtc
     9901 aggcaatccg tttgatcagt cttcacagcc gcagcaacag ccgcaacagc aacctgctca
     9961 gcaagagcag aaagacagcg acggtgtagc cggttggatt aaagatatgt ttggtagtaa
    10021 ttaacgtcta agcgtgaaat accggatggc gagttgccat ccggtaaaat aacatcccac
    10081 ccaaagtatt aaccctttct tttcatctgg ttgtttatta acccttcagg aacgctcaga
    10141 ttgcgt
//
LOCUS       DAPGOF010000178        10104 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.179, whole genome shotgun sequence.
ACCESSION   DAPGOF010000178 DAPGOF010000000
VERSION     DAPGOF010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10104
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.179"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..1252)
                     /locus_tag="RHV13_003929"
     CDS             complement(<1..1252)
                     /locus_tag="RHV13_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000876991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDV1243406.1"
                     /translation="MKYPTGVENHGGKLRIWFVYKGVRVRENLGVPDTAKNRRVAGEL
                     RSSVCYAIKTGVFDYAKQFPSSRNLEKFGEARQDLTIKELAEKFLALKETEVAKTSLN
                     TYRAVIKNILSIIGEKNLASSINKEKLLEVRKELLTGYQIPKSNYIVTQPGRSAVTVN
                     NYMTNLNAVFQFGVDNGYLADNPFKGISPLKESRTIPDPLSREEFIRLIDACRNQQAK
                     NLWCVSVYTGVRPGELCALGWEDIDLKNGTMMIRRNLAKDRFTVPKTQAGTNRVIHLI
                     KPAIDALRSQMTLTRLSKEHIIDVHLREYGRTEKQKCTFVFQPEVSARVKNYGDHFTV
                     DSIRQMWDAAIKRAGLRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQMVFQVY
                     GKWMSENNNAQVALLNTQLSEFAPT"
     gene            complement(1287..1538)
                     /locus_tag="RHV13_003930"
     CDS             complement(1287..1538)
                     /locus_tag="RHV13_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HDV1243407.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQASARPRRHLT"
     gene            complement(1611..4082)
                     /locus_tag="RHV13_003931"
     CDS             complement(1611..4082)
                     /locus_tag="RHV13_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HDV1243408.1"
                     /translation="MSKVFICAAIPDELATREEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCIPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RANQQYTENSTGKISPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KNEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYITEY
                     LTTKGVFEDEGRNQSATDTLSSPVPETDAVETAIPDNEKTECKVEVEPSVEREGPFYF
                     LFADKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYSGSQQNTGASDTTAQ
                     PEPIKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGIQTRDSV
                     NQNQHESERNYQKAEQNSPNALQNEPETKQPESVAQQEVEKVCTACGQTGGGNCPDCG
                     AVMGDATYQETFDEEYQVEVQEDDPEEMEGAEHPHKENTGGNQHHDSDNETGETADHS
                     FKVNGHPEITSTSRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTID
                     LETAGGVIDRDTIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQVWG
                     NGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNA
                     LDDARYQAKYVSVIWQKLIPSQADS"
     gene            complement(4176..4367)
                     /gene="ydfD"
                     /locus_tag="RHV13_003932"
     CDS             complement(4176..4367)
                     /gene="ydfD"
                     /locus_tag="RHV13_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein YdfD"
                     /protein_id="HDV1243409.1"
                     /translation="MNSAFALVLTVFLVSGEPVDIAVSVHRTMQECMTAATEQKIPGN
                     CYPVDKVIHQDNIEIPAGL"
     gene            complement(4364..4552)
                     /gene="dicB"
                     /locus_tag="RHV13_003933"
                     /gene_synonym="ftsT"
     CDS             complement(4364..4552)
                     /gene="dicB"
                     /locus_tag="RHV13_003933"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HDV1243410.1"
                     /translation="MKTLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKQERELLNKI
                     CIVSMLARLRLMPKGCAQ"
     gene            complement(5039..5614)
                     /locus_tag="RHV13_003934"
     CDS             complement(5039..5614)
                     /locus_tag="RHV13_003934"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243411.1"
                     /translation="MDFDTIMEKAYEEYFEGLAEGEEALSFSEFKQALRIRMCSHNDA
                     EHKYEKQNQTAENFVLEPGETLFKIPVTCPICGFTSEELDDSCNNQETTKYVEDDTEC
                     ALRTIISTSPNSRTNKSHFERVINPLPQTNKKDAGGQKTQWNNGISVKKRRSIKDKQR
                     SANNCKRFIPVINFHVLKRIYWWLRHCLMNP"
     gene            complement(5616..5771)
                     /locus_tag="RHV13_003935"
     CDS             complement(5616..5771)
                     /locus_tag="RHV13_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HDV1243412.1"
                     /translation="MDTIDLGNSESPVCGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            complement(6069..6488)
                     /locus_tag="RHV13_003936"
     CDS             complement(6069..6488)
                     /locus_tag="RHV13_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV1243413.1"
                     /translation="MVHEDKARKEFASRLALACENAGYEQHGRQAEIARRMKLTPKAV
                     SKWFNGETIPRREKLRELATLIGTTPTYLLGEDTEESGQVRFYQELNPRQKIIIDLLD
                     ELPDSETDELLKTLEEKKQKYNAIYEELARKKKQKAS"
     gene            6568..6822
                     /locus_tag="RHV13_003937"
     CDS             6568..6822
                     /locus_tag="RHV13_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDV1243414.1"
                     /translation="MNQKTLEDVIKTVRVAVVADVCGVSQRAIYKWMDNGKLPRTEYT
                     GETNYAEKIALASNGLFSADAILTIGRNKTTTKKLMGVDS"
     gene            6819..7244
                     /locus_tag="RHV13_003938"
     CDS             6819..7244
                     /locus_tag="RHV13_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HDV1243415.1"
                     /translation="MKIKHEHIRMAMNAWAHPDGEKVPAAKITKAYFELGMTFPELND
                     DSHPEALARNTQKIFRWVEKDTPDAVEKIQALLPAIEKAMPPLLVARMRSHSSAYFRE
                     LVETRERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            7316..8386
                     /locus_tag="RHV13_003939"
     CDS             7316..8386
                     /locus_tag="RHV13_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HDV1243416.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGESRNVTHMSRRVTTDKDTDKDTDQEDQN
                     TMVHGVKNATNQAGDVQTVTPGQPAGTTPEADSAYALKADSGAVQQVMTARQEQSHQL
                     QQPEADSAIQREADRVVPENTGQPVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWK
                     ARLREGVPPEAMLDGVRRYARYLAATGKTGTEFVQRATTFFGPDRHFENPWLLPVSGT
                     NNQRCVNHISEPDNEIPPGFRG"
     gene            8427..8843
                     /locus_tag="RHV13_003940"
     CDS             8427..8843
                     /locus_tag="RHV13_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HDV1243417.1"
                     /translation="MAKVFTQEEREKIKGQVLELVRQSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPSAVDPDLIWSLPDG
                     EIRRYDRRHNMICTECRKSEVMQRILSFYQGDVRFY"
     gene            complement(9111..>10104)
                     /locus_tag="RHV13_003941"
     CDS             complement(9111..>10104)
                     /locus_tag="RHV13_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000982358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243418.1"
                     /translation="EEHSSLRRLPINASNDNLSEDNIIDSMVAADLINQDKHSVAIVS
                     DMSTFVHVGDLVTFNLLDGFQLVEVKTGEKNNELYEAAEFSVISECPHFEENFINNMP
                     DNDVKQFNRIKRQIIRGMNVLEAINTGEGFDNFHQSKVKINEIDHPSEFYTHRLVKMR
                     EIIRGGKNWAIDTIDECLFLGMYRDSEMGFVAFNGWMDSLGIKSPVVNINDSFFDPLS
                     RPFMSLHLPTEMLSDLMSGQIIIVMCFDNELFFHRANKTYPGLYLLSDAARTKQPLEN
                     ILRVGSQGIASYVDGNTSFLGNGIESRILFDQQRPDNIIEWSYARSDLKKQHEA"
BASE COUNT         2545 a         2311 c         2339 g         2909 t
ORIGIN      
        1 tggttggggc aaactcgctt aactgtgtat ttaacaaagc tacctgtgca ttattgtttt
       61 cagacatcca ttttccgtat acctgaaata ccatttgcgc atctgcatgg cccatctggt
      121 ttgctataaa tgccgggtta gcaccagctg tcagcgacca gcaggcataa gtatgtctcg
      181 actgatatga ttttcgatgg cggagtccgg cacgttttat cgctgcgtcc cacatctgcc
      241 ttattgagtc aacggtaaaa tggtcaccat aattttttac tctcgctgac acttcaggtt
      301 gaaaaacaaa ggtgcatttt tgtttttctg ttctgccata ctctctgagg tgaacatcaa
      361 tgatatgctc tttgctcagt ctcgttaatg tcatctgact ccggagagcg tcgattgctg
      421 gcttaataag atgaatgacc cgattggttc ccgcctgtgt ttttggtacc gtgaaacggt
      481 cttttgctaa atttctcctg atcatcattg ttccattttt cagatctatg tcctcccatc
      541 caagtgcaca cagctcacca gggcgaacgc cagtataaac agaaacacac cataaatttt
      601 ttgcttgctg atttctgcac gcatcgataa gacggataaa ttcttcccgc gaaagaggat
      661 ccggaatggt tcttgattcc tttaatggcg agatcccctt aaacggatta tctgccaggt
      721 aaccgttatc aacaccaaac tggaacacgg cgttaagatt tgtcatgtaa ttatttacag
      781 ttacagccga tctccctggt tgtgtaacaa tatagttact tttggggatc tggtatccag
      841 tcagtaactc tttacgaacc tccagtaatt tttctttatt aatcgatgag gcaagatttt
      901 tttcaccgat tatgctcagg atatttttga tgacggcacg gtatgtgttg agtgatgttt
      961 tggcgacttc agtttctttc agtgccagaa atttttcagc cagttctttt atggttaaat
     1021 cttgtcgggc ctcaccaaat ttttccagat tgcgtgagga gggaaactgt tttgcatagt
     1081 cgaaaacacc agtttttatt gcgtaacaaa cagaggagcg tagttcacct gcaacgcgcc
     1141 tgttttttgc tgtgtcagga acccccaggt tttccctgac tcttacgcct ttataaacaa
     1201 accagatacg taatttccct ccatggtttt ccacgcctgt cggatatttc atttcaactt
     1261 ctctcattag ttagtgtggc ttttagtcaa gtaagatgac gtcttggtct cgctgatgcc
     1321 tggcgctcaa tccagcgatc aatttcttcc aggttgtaaa agcatggact gttatcccat
     1381 ggcataccgt catgagcgac atgcttatat tcccttcctt ccataaacga tttttcccgg
     1441 gcctttttta acgtaccttt ttttattcct ttcagcgcaa ttaactgctc ttcggatacc
     1501 catttgccgg gagagacaat catgattact tcgctcatcg atttctttat ctcttacatt
     1561 agacgagcgc cggttgcaga ataccagtca caaccggcga cagttgaaca ttaagaatca
     1621 gcctgactcg ggatcagttt ttgccagata actgaaacgt attttgcctg gtaacgggcg
     1681 tcatcaagtg cattatggcg ctcaccttcg aatggaatag ccgttctggc atcgaagtct
     1741 atggctttcc ccagctcaac gattgtgcgt acatcgcgat cgttgtagta acgccacggg
     1801 caggggatcc cctgccgttc gtatgaacgg cgcaaaatcg tgttgtcgaa gttggctcca
     1861 tttccccaga cctgaacaaa aaattcaccg gagttttcgt cgataaattc ccgcaattgt
     1921 aacagtgcat catctaacgg gatttcatcg gtcataatgg cagattgcgc ttcgcgtgat
     1981 tgcttaagcc accatttaat ggtgtcccga tcaatgactc cgccagcagt ttccagatcg
     2041 atagtcttac taaattccgg tcccatatct ccggtttgcg gatcgaaaaa tattgcacct
     2101 attgagatga tcggggcatc aggatttttt cccatggttt caaggtcgat cattagatgg
     2161 tcacacgtcc tgctggtgga tgtgatttct ggatgaccgt tcaccttaaa tgagtgatct
     2221 gccgtctcgc cagtttcatt atcgctatcg tgatgctgat tgccgccagt gttctccttg
     2281 tgtggatgtt cagcgccttc catttcctcc ggatcatctt cctgaacttc aacctgatac
     2341 tcttcatcga atgtttcctg gtatgttgcg tcgcccatta ccgcgccaca atcagggcag
     2401 ttgccgccgc cggtctgacc gcaggcggtg cagacttttt ccacttcctg ttgcgccact
     2461 gattcaggct gtttcgtttc tggctcgttt tgtaacgcat ttgggctgtt ttgttccgct
     2521 ttttggtagt tccgttccga ttcatgctgg ttctggttca cagaatcgcg ggtctggatc
     2581 cccttaaccc atttcggatc attcgggtca ctaatccctt caacaaattc accacgtgat
     2641 gcagcaagca acttatcggc gtcaggctgg ctgatattgg ctgcctgcat aattttgttt
     2701 acttcgtcag cggtaacttt tatcggctct ggttgtgcgg tcgtgtcaga tgcaccagta
     2761 ttttgttgtg aacctgagta tgtaccgttt tttcgggcaa aatattcttc ttttgtgatt
     2821 tcagtggcgc cagcagccag tgccttatcc agaccagaaa gtttgtttgc gcgaccgtat
     2881 ttttcgccgt ccttatctgc gaagaggaaa tagaacggcc cctcacgctc tacagatggt
     2941 tcgacttcca ctttgcattc ggttttttcg ttgtccggaa ttgccgtttc cactgcatca
     3001 gtttctggta ctggcgacga gagagtatca gttgcgctct gatttcttcc ttcatcttca
     3061 aacacgccct ttgtagtcag gtattcagta atgtatttgt tcagtgccac agggtctttg
     3121 tgaatgtcga tcggacgttc acggacaagg ccaaaaatag tctggcggtc gtagcgaagg
     3181 gcatcaggct gtttgcgcat tgatgccgag atacgcttcc agtcttcgcg gtcgttgtcg
     3241 ataacttcat tttttgccca gcgatggatg ctgccgtcaa tgtttccggc atccacatca
     3301 ccaggccaga gagcgtaggc cagttcgtca tccagtgttt tccatgtctg cttgtattcg
     3361 cgatgaatgg cagcaatgac cgggctgatt tttcctgttg aattttcagt gtactgttga
     3421 ttggctctgg cgcgggcgag atcaacaaca gacgtgtatt ttccggtttc cttgcgttca
     3481 ccttcgcgac gttttttcca gatgcgcatc tctgcctgaa tttcgggcca tttagcacca
     3541 ggaatacatt tatgcttaac ccacccaatg gcgtgcaact taagctccgg atacatggcg
     3601 ttaacttctg gcattttcat caacgcttca acgatatgtc cgtcgaatgt tgccatgtct
     3661 tcctgcaaca attcctgtgc gctaataacc atatcaacgg tgatgttttc acatgtgtcg
     3721 aacttaacca tgacagcgtt ctgtacttca ggggccagct tgtcaaaagt gacgttcatc
     3781 ggatcggatt ccgtctcaac cgggacaaaa gaagcagact cctcatccca gcggttttcc
     3841 tgcatatatt cagcatccca tgaatcgagg gcagggcggg gtatgccagg tttatcctcg
     3901 cagacaataa atttataagc gcagtcctga gcagccggat aatgttccag gaactgccag
     3961 tgaaattttg ctcgagcacg gcgttcgtcg ccagcttcaa tggctgtggc tacagccaca
     4021 gcgccttctt cccttgttgc cagttcgtca ggaatagcgg cacaaataaa gactttactc
     4081 attttgtttt aacctcatga cagatttaag gatgaacaaa tccctgccat tgctggcata
     4141 taagaatgaa accggatatt tattacggaa ctgttttaaa gacctgccgg gatttcgata
     4201 ttatcctggt gaataacttt atcgaccggg taacagttac cgggaatttt ctgttcggtt
     4261 gctgcagtca tacactcctg cattgtcctg tgaacactga ctgcaatatc aactggctct
     4321 ccggaaacaa gaaaaactgt cagaacaagc gcaaatgctg aattcattgt gcacatcctt
     4381 ttggcatcag acgtaaacga gccagcattg aaacaatgca tattttattt aatagctccc
     4441 gttcttgttt tctcttgtta atggcatctt cagtaaatac agggttactg atagtgacac
     4501 caatttcaaa acaaccttca gacgtattaa cgtttggtaa taacgttttc attatcgcgt
     4561 cctcaacaat gaattttgtg atgcagtgcc tggtgcctcc aggtgacgtt aaccagttaa
     4621 caattaacgc cggatacaga gaatccaccc ataacactgt ttttggtttt aactgttccg
     4681 cgtgcgctta gccgcattca ccgcatcaca aaattcactt taaaaacggc ggcagagcag
     4741 tcacggagta aaactgatac cgccaaacgt caccagaaaa ttgataacag agggcgttgc
     4801 agcggggttg tcacttaagc gtatggtcaa cctgacaact cggtgtcctc aacggggaag
     4861 gaataacccc gccatactta ccgccgcgcc atttcgcggg ttgccacaac cggaagcgca
     4921 cggtcgaatt aaatttaacg acaccgtaca gtgagacgaa cttcgccgtg cgctttcgtg
     4981 ttgtgtgcct gcttttaacc acgtcaggcg aggtggtatc cttaatatca ccacagtttt
     5041 aaggattcat taagcaatgt cgcaaccacc aataaatccg cttaagaaca tgaaaattga
     5101 ttactggtat aaagcgctta cagttgttgg cgctgcgttg tttgtcttta atggaacgtc
     5161 tttttttgac agatatcccg ttgttccatt gggttttttg tcctccggca tcttttttat
     5221 tggtttgggg gagtggatta atcaccctct caaagtgaga tttattggtc ctggagtttg
     5281 gactcgtgga tataatcgtt cttagtgcgc actcggtatc atcttcgaca tacttggttg
     5341 tttcctgatt gttacaggag tcgtcaagtt cttctgatgt aaaaccgcaa atggggcacg
     5401 taacgggaat tttgaaaagc gtttctccgg gttccagaac aaaattttct gcggtctgat
     5461 tttgcttctc atatttgtgc tccgcgtcat tgtgagagca cattcttatt ctgagtgcct
     5521 gtttaaactc actgaagctg agagcttctt cgccttcggc aagaccttcg aagtattctt
     5581 cgtaagcctt ttccatgatt gtgtcgaaat ccatatcact cacctgagtt tctttccagc
     5641 cagcgacggg caccattttc ggttttaaac gttttgcttt tggtatacgt catcgcggtg
     5701 aacgtaccgt cctggttggg gaacacgcca cataccggag attcgctgtt gccaagatcg
     5761 atagtatcca tgctgacctc atttcccctt aacgccgggg tagcggaaca aaaacctgct
     5821 gcatagttat taaagttgaa ccctgccgtc atgttcttac gcctcgggct ggctacttaa
     5881 cccctgacca ctgcctggta actcgaagta ttgccctgca ttctgtgggg cggggtgggt
     5941 ggcaggcata taatgtactt tgtgttcatt gttgtaaagt acttttagta cattttgtgt
     6001 gtaaaaaaat gagatgggat aaagtgaagc acaaacccgg aggaaggcgc taccggattt
     6061 atgctggttt aagaggcttt ttgttttttc tttcgtgcta actcttcgta aattgcattg
     6121 tacttctgtt ttttctcttc aagagttttt aaaagttcat ctgtctcact gtcagggagc
     6181 tcgtccagaa ggtcaatgat gattttttgt cttggattta actcctgata gaaacgtacc
     6241 tgtccacttt cttctgtatc ctctcccaaa agataggttg gtgttgttcc tattagtgtt
     6301 gctaattccc ttaatttctc ccggcgagga attgtttcgc cattaaacca tttgctaacc
     6361 gcttttggtg ttaatttcat tcgacgggca atttctgcct gccttccatg ttgttcataa
     6421 ccagcgtttt cacaggctag cgcaagccta ctggcgaact ctttacgcgc tttatcttca
     6481 tgaaccataa gttcaatgat attcgctctt gaatgtactg tcagttctgt tatagcatgt
     6541 actcaaagtt cacattgtga gggtgatatg aaccagaaaa cacttgaaga tgtaatcaaa
     6601 actgttcgcg ttgctgttgt ggccgacgtt tgtggtgtca gccaaagagc aatctataaa
     6661 tggatggata acggaaaatt gcctcgcaca gaatataccg gcgaaacaaa ttacgctgaa
     6721 aaaatcgctc ttgcatcaaa cggattattt tctgccgatg caattttaac tattggcagg
     6781 aataaaacta ctacgaaaaa gctgatggga gttgattcat gaaaatcaag catgaacaca
     6841 tccgcatggc gatgaatgcc tgggcgcatc cggacggcga aaaagtaccg gctgcgaaaa
     6901 ttaccaaagc gtatttcgag ctgggaatga cgttcccgga actgaatgac gacagccatc
     6961 cggaagccct ggctcgcaat acccagaaaa ttttccgctg ggtagagaaa gacacccctg
     7021 atgcagttga aaaaattcag gcgttgttac cagcgatcga aaaggcaatg ccacctttgc
     7081 tggtggccag aatgcgcagc cacagttcag cttattttcg ggagctggtg gagacgcggg
     7141 agcgactggt gagagacgct gatgattttg tcgcagtggc aatcgccggt ttcaatcaga
     7201 tgaaccgtgg tggcccggca ggaaatgctg tggcagtaca ttgactgaca atagccatat
     7261 cgaatcgctt ccggcaactc gtgagtaaaa agattcggta tcagaagagg tgagtatggc
     7321 taacgcctgg ctcagattat ggcatgacat gccaaatgac cctaagtggc gaacaattgc
     7381 cagggtgtca gggcagccaa ttgcaacagt gatggcagtg tatatccacc tcctggtgag
     7441 cgcgtcacga aatgtcacgc gaggtcacat tgatgtcacg acagaagatt tggcaagtgc
     7501 gctcgacgtg acagaagagg taattgattc aattttgcag acgatgcagg ggcgggtact
     7561 tgatggtgat ttaatcactg gatgggaaaa acgccaggtg ctgaaagagg acaacggcaa
     7621 tatttcgcaa accgcaaaat ctccggcaga gcgcaagagg gcgcagcgag agagggaaag
     7681 aaagcgggaa caaaatggcg attgtcacgg cgagtcacga aatgtcacgc acatgtcacg
     7741 acgagtcacg acagataaag atacagataa agatacagat caagaagatc aaaacactat
     7801 ggtccatggc gtaaaaaacg ccacgaacca ggcaggggat gttcagaccg tcactcctgg
     7861 ccagcccgca ggcacgacac cggaagccga ttcagcgtat gcgctgaaag ccgattcggg
     7921 cgctgtgcag caggtgatga ccgcaaggca ggagcaatca caccaacttc agcagcctga
     7981 agccgattcc gccattcagc gggaagccga tcgggtagtc ccggaaaaca ccgggcagcc
     8041 tgtgggacga gtggattatc ctgatgtgtt cgaacaggtc tggcgggaat acccgttgcg
     8101 tgccggggca aacccgaaga aatccgcttt cagtgcctgg aaagccagat tacgcgaggg
     8161 ggtgccacca gaggccatgc tggatggtgt gaggcgttac gcaagatacc ttgcggctac
     8221 cgggaaaacg ggaacggaat ttgttcagcg agcgacgacg ttttttggac cggaccggca
     8281 ttttgaaaac ccctggctgc tcccggtaag cggcacgaac aaccagcgtt gtgtgaatca
     8341 tatttctgaa ccggataacg aaattccgcc gggcttcagg gggtaagtgt taatttctgg
     8401 tcatgaggta attttcagga gggcttgtgg caaaagtttt tacacaagaa gagcgggaaa
     8461 aaattaaagg acaggttctt gaactcgtac gccagagtgg gcgcgagacg ttacgacaac
     8521 tggaagctaa aactggggca acaagatatc tgatgagcgt tctggccaga gagctggttg
     8581 ccagtggcga tgtatacaat tctggctacg ggttattccc gtctgaacag gcgcgtaagg
     8641 actggcaaaa tgcccgtaaa aagctctcaa gggcaaagct gaagaaacca tctgcggttg
     8701 atccggacct tatctggtca ttacctgatg gagaaatacg tcgttacgac aggcgtcata
     8761 atatgatttg tactgagtgt cgtaaaagcg aagttatgca gcgcatattg tcgttttatc
     8821 agggggatgt tcggttttat tgaagtgacg agattaaagt gcattagttc agatgcaaat
     8881 tgacattttg tggcacaggg tagagctagc gtggttgtcc gctttgtgcc aacagcggac
     8941 attatggaag gtcagagtta aagattaaaa tgggatgctg tgtgttttct ccaggttttg
     9001 ctctatacct ttcgggatac tccaccgaca gaatacgaaa atcaacatta tcaacggctc
     9061 gaagtgtcta gattatccat ggcaattcat gggaaatccc atccaacggc ttatgcttca
     9121 tgttgctttt tgagatcgct tctagcatag ctccactcaa taatgttgtc gggtcgttgc
     9181 tgatcgaaaa ggatacggga ttcaatacca ttacctaaaa agctggtatt cccatctacg
     9241 taagaagcta tcccttgtga accaacacga agaatatttt ctaatggctg ctttgttctt
     9301 gctgcatcgc taaggagata taaacccggg tacgttttgt tagcacggtg aaaaaaaagt
     9361 tcattgtcaa aacacatgac gatgattatt tgtcccgaca tgagatcgga gagcatttca
     9421 gttggcagat gcaaactcat aaatggtcga gacaaagggt caaagaatga atcatttata
     9481 ttcacaacag ggctttttat accgagacta tccatccatc cattgaaggc aacaaagccc
     9541 atttcagaat cacggtacat acctaagaac aaacattcat caatagtatc aattgcccaa
     9601 ttttttcctc cgcgaataat ctcccgcatt ttaactaagc ggtgggtata aaattcagac
     9661 ggatgatcta tctcattgat tttaacttta gactgatgaa agttgtcaaa gccctcgcca
     9721 gtattaatcg cctccagaac attcatgcca cgaatgattt gcctttttat gcgattaaat
     9781 tgcttaacat cattatcagg catattgtta atgaagtttt cttcaaagtg cggacattct
     9841 gagataacag aaaattcagc agcttcataa agctcattat tcttttcacc tgttttaact
     9901 tccacgagct ggaagccatc cagtagatta aaggtgacta aatctcccac atgaacaaat
     9961 gtgctcatgt cagagacaat cgctacggag tgtttgtcct ggttgataag atcggctgcg
    10021 accatagaat caattatgtt atcttctgat agattgtcat tgcttgcatt aataggtaaa
    10081 cgccgcaaac tagagtgttc ttca
//
LOCUS       DAPGOF010000179        10005 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.180, whole genome shotgun sequence.
ACCESSION   DAPGOF010000179 DAPGOF010000000
VERSION     DAPGOF010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.180"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            451..1428
                     /gene="csiD"
                     /locus_tag="RHV13_003942"
     CDS             451..1428
                     /gene="csiD"
                     /locus_tag="RHV13_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HDV1243419.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRIMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDYYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDIDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYASNHYQTHQ"
     gene            1448..2716
                     /gene="lhgO"
                     /locus_tag="RHV13_003943"
     CDS             1448..2716
                     /gene="lhgO"
                     /locus_tag="RHV13_003943"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HDV1243420.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERM
                     RALWERTAANGIEREWLNADELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2739..4187
                     /gene="gabD"
                     /locus_tag="RHV13_003944"
     CDS             2739..4187
                     /gene="gabD"
                     /locus_tag="RHV13_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HDV1243421.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            4201..5481
                     /gene="gabT"
                     /locus_tag="RHV13_003945"
     CDS             4201..5481
                     /gene="gabT"
                     /locus_tag="RHV13_003945"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HDV1243422.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMQRLRTLCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHTEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIIGQCFAEAKQ"
     gene            5719..7119
                     /gene="gabP"
                     /locus_tag="RHV13_003946"
     CDS             5719..7119
                     /gene="gabP"
                     /locus_tag="RHV13_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HDV1243423.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYADVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            7140..7802
                     /gene="csiR"
                     /locus_tag="RHV13_003947"
     CDS             7140..7802
                     /gene="csiR"
                     /locus_tag="RHV13_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HDV1243424.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7803..8252)
                     /gene="kbp"
                     /locus_tag="RHV13_003948"
     CDS             complement(7803..8252)
                     /gene="kbp"
                     /locus_tag="RHV13_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HDV1243425.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8336..8494)
                     /locus_tag="RHV13_003949"
     CDS             complement(8336..8494)
                     /locus_tag="RHV13_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HDV1243426.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            8677..8976
                     /locus_tag="RHV13_003950"
     CDS             8677..8976
                     /locus_tag="RHV13_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV1243427.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            8986..9510
                     /gene="ygaP"
                     /locus_tag="RHV13_003951"
     CDS             8986..9510
                     /gene="ygaP"
                     /locus_tag="RHV13_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HDV1243428.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(9557..9961)
                     /gene="stpA"
                     /locus_tag="RHV13_003952"
     CDS             complement(9557..9961)
                     /gene="stpA"
                     /locus_tag="RHV13_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HDV1243429.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2292 a         2577 c         2727 g         2409 t
ORIGIN      
        1 cccccctggt aaagcattta tgcttagagg cggaactcta aatatcaata ttcctgggcg
       61 tagcgctctt cttttaggga aaacaggaga accgctgaac tatctctatt tgtaatttaa
      121 tgttatatct gccccgctaa aacggggcag ataatatgtt tagtttacta gcggtcattt
      181 tgcagtgaag ccatttacag ttttttatcg accagataat ctgttctctg atggtaatcc
      241 cccctaacct cttgctttag ttattcattt cctgtctcac tttgccttaa taccctacgt
      301 taaatgttac caatttgttg tttttgatca caataagaaa acaatatggc gcatttgtgc
      361 gcatttttca gaaatgtaga tatttttaga ttatggctac gaaatgagca tcgccatgtc
      421 accctacatc tcataagagg atcgcttctg atgaatgcac tgaccgccgt acaaaataac
      481 gctgtcgatt caggccagga ctatagcgga ttcactctca tcccgtcggc gcaatccccg
      541 cgtctgctgg aactcacctt caccgaacag acgaccaaac agtttctcga acaggttgcc
      601 gaatggcccg tgcaggcgct ggagtacaaa tcttttctgc gttttcgggt aggcaaaatt
      661 cttgacgatc tgtgtgcgaa tcagctgcaa ccattgctgt tgaagaccct gctaaaccgt
      721 gctgaaggtg cgctgttgat caatgcggtg ggtatcgatg atgtggctca ggcagatgag
      781 atggtgaagc tggcaacggc ggtagcgcat ctgattggcc gttccaattt tgatgcgatg
      841 agcggtcagt attacgcgcg ttttgtagtg aaaaatgtcg ataactcaga cagctatctg
      901 cgtcagccgc accgcataat ggagctgcac aacgacggca cctacgtcga agagatcacc
      961 gactatgtgc tgatgatgaa aatcgacgag cagaacatgc agggtggaaa ttcgttgctg
     1021 ctgcatctcg atgactggga acatctggac tactatttcc gccacccgct ggcgcgtcgc
     1081 ccgatgcgct ttgccgcgcc tccgagcaaa aacgtcagca aagatgtttt ccatccggtg
     1141 ttcgacattg atcagcaggg ccgcccggtg atgcgctata tcgaccagtt tgtccagccg
     1201 aaagacttcg aagaaggcgt gtggttgagc gagctttcgg acgccattga aaccagtaaa
     1261 ggcattcttt ctgtacccgt tcccgttggc aaattcctgt tgattaacaa cctgttctgg
     1321 ctgcacggtc gcgaccgctt tactccgcac ccggatctgc gccgtgaact gatgcgccag
     1381 cgtggctatt tcgcttacgc ctctaaccac taccagacgc atcagtaagc gcaaaggaat
     1441 tgagcggatg tatgattttg tgattattgg cggtggcatc atcggcatgt cgaccgccat
     1501 gcaactgatt gatgtctatc cggatgcccg cattgcgttg ctggaaaaag agtccggccc
     1561 ggcctgtcac cagacgggcc acaacagcgg cgtgatccat gccggggtct attacacgcc
     1621 cggcagcctg aaggcgcagt tttgcctggc gggaaaccgc gccactaaag ccttttgcga
     1681 tcaaaacggc attcgctacg acaactgcgg caagatgctg gtcgccacgt ctgaactcga
     1741 aatggaacgg atgcgcgcct tatgggaacg cacggcggcg aacggcatcg agcgcgagtg
     1801 gttaaacgcc gatgaactgc gcgagcgcga accgaatatc accgggcttg gcggtatttt
     1861 tgtgccgtcc agcggcattg tcagctaccg cgaagtcacg gcggcgatgg caaaaatctt
     1921 ccaggccaga ggcggcgaga ttatctataa cgccgaagtc agcgccctca gtgagcataa
     1981 aaacggcgtg gtgatacgta cccgtcaggg cggtgaatat gaagcatcga cgctgattag
     2041 ctgctccggg ctgatggctg accggctggt gaaaatgctc ggcctcgaac cgggctttat
     2101 tatctgcccg ttccgtggcg agtatttccg cctggcgccg gagcataacc agattgttaa
     2161 ccatctgatt taccccattc ccgatccggc aatgccgttt ttgggcgttc atctcacccg
     2221 catgatcgac ggtagcgtca ccgttgggcc aaacgcagtg ctggcgttca aacgcgaagg
     2281 ctatcgcaag cgcgacttct catttagcga cacgctggag attttaggct cgtcggggat
     2341 tcgccgggtg ctgcaaaacc atctacgctc aggactgggc gagatgaaaa actcgctgtg
     2401 caaaagcggc tatctgcggc tggtgcaaaa gtattgtccc cggctttcgt taagcgatct
     2461 ccagccctgg cccgccggtg tgcgggcgca ggcggtatcg ccggacggca agctgattga
     2521 cgattttctg tttgtcacca ccccgcgcac gatccacacc tgcaatgcgc cctccccggc
     2581 agcgacatca gcaattccta ttggtgcgca tattgtcagc aaggtacaaa cgctgttggc
     2641 aagccagagt aaccccggac gcacgctgcg agcggcacgt agtgtggatg ccttacacgc
     2701 cgcatttaat caataacctt tgaaaacagg acgtaacaat gaaacttaac gacagtaact
     2761 tattccgcca gcaggcgttg attaacgggg aatggctgga cgccaacaat ggtgaagtca
     2821 tcgacgtcac caatccggcg aacggcgaca agctgggtag cgtgccgaaa atgggcgctg
     2881 atgaaacccg cgccgctatc gacgccgcca accgcgccct gcccgcctgg cgtgcgctca
     2941 ccgccaaaga acgcgccaac attctgcgca actggttcaa tttgatgatg gagcatcagg
     3001 acgatttagc gcgcctgatg accctcgaac agggcaaacc actggccgaa gcgaaaggtg
     3061 aaatcagcta cgccgcctct tttattgagt ggtttgccga agaaggcaaa cgcatttatg
     3121 gcgacaccat tcccggtcat caggccgata aacgcctgat tgttatcaag cagccgattg
     3181 gcgttaccgc agctatcacg ccgtggaact tcccggcggc gatgattacc cgtaaagccg
     3241 gtccggcgct ggcggcaggc tgcaccatgg tgctgaagcc cgccagtcag acaccgttct
     3301 ctgcgctggc gctggcggag ctggcgattc gcgcgggcat tccggctggc gtgtttaacg
     3361 tggtcaccgg ttcggcaggc gcggtcggta atgaactgac cagcaacccg ctggtacgca
     3421 aactgtcgtt taccggttcg accgaaattg gccgccagtt aatggaacag tgcgcgaaag
     3481 acatcaaaaa agtgtcgctg gagctgggcg gcaacgcgcc gtttatcgtc tttgacgatg
     3541 ccgatctcga taaagccgtg gaaggcgcgc tggcctcgaa attccgcaac gccgggcaaa
     3601 cctgcgtctg cgccaaccgt ttatacgtgc aggacggcgt gtatgaccgc tttgccgaaa
     3661 aattgcagca ggcggtgagc aaactgcaca tcggcgacgg gctggataaa ggcgtcacca
     3721 tcgggccgct gatcgatgaa aaagcggtag caaaagtgga agagcatatt gccgatgcgc
     3781 tggagaaagg tgcgcgtgtg gtttgcggcg gtaaagcgga tgaacgcggc ggcaacttct
     3841 tccagccgac cattctggtg gacgtgccgg ccaacgccaa agtgtcgaaa gaagagacgt
     3901 tcggccccct cgccccgctg ttccgcttta aagatgaagc cgatgtgatt gcgcaagcca
     3961 atgacaccga gtttggcctt gccgcctatt tctacgcccg tgatttaagc cgcgtcttcc
     4021 gcgtgggcga agcgctggag tacggcatcg tcggcatcaa taccggcatt atttccaatg
     4081 aagtggcccc gttcggcggc atcaaagcct cgggtctggg tcgtgaaggt tcgaagtatg
     4141 gcatcgaaga ttacttagaa atcaaatata tgtgcatcgg tctttaactg gagaatgcga
     4201 atgaacagca ataaagagtt aatgcagcgc cgcagtcagg cgattccccg tggcgttggg
     4261 caaattcacc cgattttcgc tgaccgcgcg gaaaactgcc gggtgtggga cgttgaaggc
     4321 cgtgagtatc ttgatttcgc gggcgggatt gcggtgctca ataccgggca cctgcatccg
     4381 aaggtggtgg ccgcggtgga agcgcagttg aaaaaactgt cgcacacctg cttccaggtg
     4441 ctggcttacg agccgtatct ggagctgtgc gagattatga atcagaaggt gccgggcgat
     4501 ttcgccaaga aaacgctgct ggttacgacc ggttccgaag cggtggaaaa cgcggtaaaa
     4561 atcgcccgcg ccgccaccaa acgtagcggc accatcgctt ttagcggcgc gtatcacggg
     4621 cgcacgcatt acacgctggc gctgaccggc aaggtgaatc cgtactctgc gggcatgggg
     4681 ctgatgccgg gtcatgttta tcgcgcgctt tatccttgcc cgctgcacgg catcagtgaa
     4741 gatgacgcta tcgctagcat tcaccgaatt tttaaaaatg acgctgcgcc ggaagatatc
     4801 gccgccatcg tgattgagcc ggttcagggc gaaggcggtt tctacgccgc gacgcctgct
     4861 tttatgcagc gtttacgcac gctgtgtgac gagcacggga tcatgctgat tgccgatgaa
     4921 gtgcagagcg gcgcagggcg taccggcacg ctgtttgcga tggagcaaat gggcgtggcg
     4981 ccggatctca ccacgtttgc gaaatcgatc gcaggcggtt ttccgctggc gggcgtcacc
     5041 gggcgcgcgg aagtgatgga tgccgtcgct ccaggcgggc tgggcggcac ctatgccggt
     5101 aatccgattg cctgcgtggc ggcgctggaa gtgttgaagg tgttcgagca ggaaaatctg
     5161 ctgcagaaag ccaacgatct ggggcagaag ctgaaagacg gattgctggc gatcgccgaa
     5221 aaacacacgg agatcggcga cgtacgcggt ctgggggcga tgatcgccat cgagctgttt
     5281 gaagatggcg atcacagcaa accggacgca aaactcaccg ccgagatcgt ggcgcgcgcc
     5341 cgcgataagg gcctgattct tctctcctgc ggcccgtatt acaacgtgct gcgcatcctt
     5401 gtaccgctca ccattgaaga cgctcagatc cgtcagggtc tggagatcat cggccagtgt
     5461 tttgctgagg cgaagcagta gcaccgcccc catgccggag gcgacgctgc gcgtcttgtc
     5521 cggcctacgg ggatccaggt cggataaggc gtttacgccg catccgacaa tctgtacgtg
     5581 aacaggaata aatctatgtc ggccggataa ggcggagccg ctctccggca aaaagaatca
     5641 ataacaatta aacgcgtgac ccgacgcggg aaatgtcggg gcgctctccc aagtgacaca
     5701 ctttcgagag gattcaggat ggggcaatca tcgcaaccac atgagttagg cggcgggctg
     5761 aaatcacgcc acgtcaccat gttgtctatt gccggtgtta tcggcgcaag tctgtttgtc
     5821 ggttccagcg tcgccatcgc cgaagcgggc ccggcggtat tactggccta tctgttcgcc
     5881 gggctactgg tggttatgat tatgcggatg ttggcggaaa tggcggttgc cacgcccgat
     5941 accggttcgt tttccaccta tgccgataaa gctattggtc gttgggcggg ttataccatc
     6001 ggctggttgt actggtggtt ttgggtactg gttatcccgc tggaagccaa catcgccgcc
     6061 atgatcctgc actcgtgggt tccaggcatt cccatctggt tattttccct cgtcattacc
     6121 cttgccttaa ctggcagtaa tttattaagc gttaaaaact acggcgaatt tgagttctgg
     6181 ctggcgctgt gcaaagtcat cgctatcctg gcctttattt tccttggtgc agtcgcaatt
     6241 agcggttttt acccgtatgc cgacgtgagc gggatctcaa gattgtggga tagcggcggc
     6301 tttatgccca acggcttcgg tgcggtatta agcgcgatgt tgatcaccat gttctcgttt
     6361 atgggcgcag aaattgtcac cattgccgcc gcggaatccg acacgccgga aaaacatatt
     6421 gtccgcgcca ccaactcggt tatctggcgt atttctatct tctatttatg ttctattttt
     6481 gtcgtcgtgg cattaattcc gtggaatatg cccggactaa aagccgtcgg ttcttatcgc
     6541 tcggtactgg aattgctcaa tattccccat gcgaaattga tcatggactg cgtgatatta
     6601 ctttccgtaa ccagttgcct gaactcggct ctgtataccg cttccaggat gctctactcc
     6661 ttaagccgtc gcggagatgc gcctgcggta atgggtaaaa tcaaccgcag taaaaccccg
     6721 tacgtggcgg tgttactctc caccggcgcg gcattcttaa cggtggtggt gaactattac
     6781 gcgcctgcga aggtatttaa atttctgatc gacagctccg gcgctatcgc cctgctggtt
     6841 tatttagtca tcgccgtttc acagttgcgg atgcgcaaaa ttctgcgagc agaaggaagc
     6901 gaaattcgct tgcgaatgtg gctttatcct tggctcacct ggctggtcat cggctttatt
     6961 acctttgtgt tggtagtgat gctattccgt ccggcgcaac agttagaagt gatctccacc
     7021 ggcttattag cgatagggat tatctgtacc gtgccaatta tggctcgctg gaaaaagctg
     7081 gtattgtggc aaaaaacacc cgttcataat acgcgctgat aatgatcagg agtcacacca
     7141 tgaccattac gtctctggat ggctatcgct ggctgaagaa cgatattatt cgcggtaatt
     7201 ttcaaccgga tgaaaaatta cggatgagtt tgctgacatc gcgttatgca cttggcgttg
     7261 ggccgttgcg ggaagcactt tctcaactgg tggcggaacg gctggtcacg gtggtgaatc
     7321 aaaaagggta tcgggtggcg tctatgtcag agcaggagct gctcgatatt ttcgatgccc
     7381 gcgccaatat ggaagcgatg ttagtgagtc tggcgattgc ccgcggtggc gatgaatggg
     7441 aggcagacgt tctcgcaaaa gcgcatctgc tcagtaagct ggaagcctgt gacgccagtg
     7501 agaaaatgct tgatgagtgg gatctgcgtc atcaggcgtt tcatacagca attgtggcgg
     7561 gctgtggttc tcactatttg ctgcaaatgc gtgaacggtt gtttgatctg gcggcgcgct
     7621 atcgatttat ctggctgcgg cgaacggtgc tttcggtaga aatgctggag gataaacacg
     7681 atcagcacca gaccctgact gcggcggtac tggcgcgaga taccgcgcgc gccagtgagt
     7741 taatgcgcca gcatttactg acgccaattc ccattatcca gcaggcgatg gctggcaatt
     7801 aactactctt ccggaatacg caacacttgc cccggataaa ttttatccgg gctttttagc
     7861 atcggtttat tcgcttcgaa gattttattg tacagattag cgttaccgta gacctgtttg
     7921 gaaatggcac tcagagtgtc gccagactta acggtataaa actggctggc agtggctggt
     7981 gtcgccgttt tcacctgatc atcaacactg gcaataccgg aaatattccc caccgcaaca
     8041 aggattttct ccttcgcctc ctgactcagg ccgtcaccag tgaccgtcgc tttgccgtcg
     8101 gcaatttgaa tgttcacttt atcggcatca ggtataccgg ttttgctcag atgctcctgc
     8161 accttcttcg cctgatcgtc tttatcgtgc tgacctgtaa ccgcgtccca gagtttttct
     8221 ccggcatctt tcacaaaatt gaacagaccc atagctacct cattcgttaa cggaaacact
     8281 cagaaagtgt agcagaggaa cgcggcgcgt taacgccgcg ggggtgagca caggtttaat
     8341 cgcgggtttg cacccagaac gcgtgaatca aaccggggat atacccaagc agtgtcagca
     8401 gaatattaat aatgaacgcc caaccgaacc ctttaccgag cagcacgccg agcggcggca
     8461 gaatgatggt gatgacgatt ctccagaaac ccatatgtac tccctataag aaaattactc
     8521 attgtttaaa aagagatttt atctcttaag cgtagtaatt ttagcggagc ctgccagttt
     8581 cagcttacgc ttttactctc atttacgctg ctgaggctgg tatatgttgc atttttagtt
     8641 tagagtttac ttaatttaga aaatacttaa ataaatatga ctgaactcgc gcaattacag
     8701 gccagtgccg aacaggcagc ggccttattg aaagcaatga gccatcctaa acgattgctg
     8761 attctgtgca tgcttagcgg ttcccccggc accagcgcgg gagagctgac gcgcattacc
     8821 ggactgagtg cctctgcgac atcacagcat ctcgctcgta tgcgggacga agggcttatc
     8881 gacagccaac gggatgccca acgcattcta tattccatta aaaatgaggc ggtaaatgcc
     8941 attatcgcca ccctgaaaaa tgtctattgt ccgtaaggag tcactatggc tttgacaacc
     9001 atttcgccgc atgatgcaca agaattaatc gcacgcggcg caaagttaat cgatattcgt
     9061 gatgctgatg aatatcttcg tgaacatatt cctgaagcag atctggctcc attatccgtg
     9121 ctggaacagt caggtcttcc acctaaatta cgtcgcgagc aaattatatt ccactgccag
     9181 gcaggtaaac gcaccagtaa taacgccgat aaattagcag cgattgccgc ccccgcagaa
     9241 atatttttac tcgaagatgg gattgatggc tggaaaagag ctggattgcc agtagcggta
     9301 aataaatctc aacccttgcc gttaatgcgc caggtgcaga tcgctgcggg tggtttaata
     9361 ttaatcggcg ttgtactggg ttataccgta aatagcggtt tcttcttatt aagtggcttt
     9421 gttggtgccg ggttactgtt tgcaggaatc agcggttttt gtggaatggc aaggttgtta
     9481 gataagatgc cgtggaacca acgagcttga gaagcgacgc cggacgcgcc ctagcagcga
     9541 catccggcct cagtaattag atcaggaaat cgtcgagaga tttaccttct gccagcgcct
     9601 gagcaattgg cttcggtgta cggccctgac cggtccaggt tttagtttca ccgttaacat
     9661 cggtgaattt atatttcgcc ggacgcggct ggcgtttttt accagcgcgt ggtgcagcag
     9721 cagagctatt acccaataac tcttccgggt taattccgtc agccttcatc agctccagcc
     9781 aggtgctaat tttttcctga cgctcagcca gttcacgctg ctgctgttct tcttcttcac
     9841 gtctttcttt agtgacaacc ctgaattttt cgagcatttc ttcaagaacg tcaatggaga
     9901 attcgcgagc catcgcacgg agggtgcgaa tgttatttaa actttgtaac attacggaca
     9961 taataaagaa aaccttttaa cgccaaaaca aaaaagtatt tcgtc
//
LOCUS       DAPGOF010000180         9936 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.181, whole genome shotgun sequence.
ACCESSION   DAPGOF010000180 DAPGOF010000000
VERSION     DAPGOF010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.181"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            311..3220
                     /locus_tag="RHV13_003953"
     CDS             311..3220
                     /locus_tag="RHV13_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDV1243430.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANATTYDTWTYVDNAATGLN
                     WTNMDNPGTVDGNYVNYSGFVYYNNTNGDFDQSFNGDTVNGTISTYYLNHDYTDGTNG
                     NANQLDIANSVIHGSITSMLPGGDLWHNGAYDQSYGYIQAGDTVIDTTWYDGDVFTLN
                     IANSTIDDDYEALYFTDYYKDGDVTKSTNGTFDVSEGVAVNLNVESNINISNNSRVAG
                     IALSQGNTYNNTYTTESHTWDNNINVSNSTVTTGSNTVLESSGYGHFGNSGEPSDYAG
                     PGDVALSFTDSTSDYAMKNNVYFSNSTLMGDVAFTSTWNANFDPSGHDSNGDGVKDTN
                     AGWVDDSLNVDELNITLNNGSKWVGQATFNAETISPDTMYDVATNSLTPGGTAEANGW
                     NRIIDNKVFQSGVFNVALNNGSEWDTTGDSLVDTLTVNNGSQVNVSDSSLTSDTIDLT
                     NGSSLNIGESGYVDTDHLTINSYSSVDLTESTGWGIYSNLYANTITVTNGGVLDVNVD
                     QFDTEAFRTDKLELTSGNIADNNGNVVSGVFDIHSSDYVLNADLVNDRTWDTSKSNYG
                     YGIVAMNSDGHLTINGNGDMNNGDELDNSSVDNVVAATGNYKVRIDNATGAGAIADYK
                     DKEIIYVNDVNTNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSA
                     NTNIWNLEQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGV
                     DTKIDGNNAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSY
                     SHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYGSISGLFQSGD
                     DYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVN
                     GDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
     gene            3308..3931
                     /gene="iprA"
                     /locus_tag="RHV13_003954"
     CDS             3308..3931
                     /gene="iprA"
                     /locus_tag="RHV13_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HDV1243431.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEDVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(3932..5089)
                     /gene="ampH"
                     /locus_tag="RHV13_003955"
     CDS             complement(3932..5089)
                     /gene="ampH"
                     /locus_tag="RHV13_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HDV1243432.1"
                     /translation="MKRSLFFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            5441..6661
                     /gene="sbmA"
                     /locus_tag="RHV13_003956"
     CDS             5441..6661
                     /gene="sbmA"
                     /locus_tag="RHV13_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HDV1243433.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFALFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            6674..7768
                     /gene="yaiW"
                     /locus_tag="RHV13_003957"
     CDS             6674..7768
                     /gene="yaiW"
                     /locus_tag="RHV13_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HDV1243434.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMLASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPPFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAAKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(7827..8135)
                     /locus_tag="RHV13_003958"
     CDS             complement(7827..8135)
                     /locus_tag="RHV13_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HDV1243435.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            8395..8607
                     /locus_tag="RHV13_003959"
     CDS             8395..8607
                     /locus_tag="RHV13_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HDV1243436.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            complement(8631..9725)
                     /gene="ddlA"
                     /locus_tag="RHV13_003960"
     CDS             complement(8631..9725)
                     /gene="ddlA"
                     /locus_tag="RHV13_003960"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDV1243437.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
BASE COUNT         2553 a         2313 c         2438 g         2632 t
ORIGIN      
        1 tcaaaaagtg aataacttaa agttcattgc ttattgatac attatttgtc tgttttagtt
       61 gttttatatt ttcatggtct atgttttaaa attaacgctt tcattaacgt attgaaatat
      121 tgtgtttttg ttatttcttt ctgtgtgtgt gttcacgtgc tttatatatg tccgcaatcg
      181 gacttagggg aaagaataat tgaaccattc ctctataacg ccgcataatc gtcaacgcga
      241 aacataatat gttttctatg cattgataat tgatggatca acttattacg tccctgagga
      301 gggatgacaa atgcactcct ggaaaaagaa acttgtagta tcacaattag cattggcttg
      361 cactctggct atcacctctc aggctaatgc aactacttat gacacctgga cttatgtcga
      421 taacgcggct actggtctta actggaccaa tatggataat ccgggtactg tagatggtaa
      481 ctatgtgaat tatagtggct ttgtctacta taacaacacc aatggcgact tcgatcagtc
      541 ttttaacggt gatactgtta acggcacgat ttctacatac tatctgaacc acgactacac
      601 tgacggtaca aatggtaatg ctaaccagct tgatattgct aattcagtaa ttcatggctc
      661 aatcacttcc atgcttcctg ggggtgatct ctggcataat ggtgcttatg atcaaagcta
      721 tggctacatt caggctggtg atacagttat tgatacaacg tggtatgacg gtgatgtatt
      781 cactctgaat attgctaact ccaccattga tgatgactat gaagcacttt acttcactga
      841 ttactacaaa gatggcgatg taactaaatc aacgaatggc acctttgacg ttagcgaagg
      901 cgttgctgtt aatcttaatg tagaaagtaa catcaatatc tccaataact cccgcgttgc
      961 aggtattgcg ttatctcaag gtaatactta caacaatacc tacaccactg aatcccatac
     1021 ttgggataac aatattaatg tctctaactc aaccgtaact accggttcaa ataccgtgtt
     1081 ggaaagttct ggttatggtc atttcggtaa ctctggtgag ccgagtgatt atgctggccc
     1141 gggtgacgtt gcattgtctt tcactgatag tacttcagac tatgcaatga agaacaatgt
     1201 atatttcagc aattctactc tgatgggtga tgttgcgttt accagcacct ggaatgctaa
     1261 ttttgatcca agtggtcatg attctaacgg tgacggcgta aaagatacca atgctggttg
     1321 ggttgatgat agcctgaacg ttgatgagct gaatattact cttaataatg gtagcaagtg
     1381 ggttggtcag gctacattca atgctgaaac catttctcca gacacaatgt atgatgttgc
     1441 aactaacagt ttgacccctg gtggcacagc tgaagctaat ggttggaatc gcatcatcga
     1501 caataaagta ttccagagtg gtgtatttaa cgtagcgttg aataacggtt ctgaatggga
     1561 tactacaggt gattcgcttg ttgataccct gaccgttaat aatggttctc aagtcaatgt
     1621 ttctgattct agtttaactt cagacactat cgatttaact aatggctctt ctctgaacat
     1681 tggtgaaagt ggttatgttg ataccgatca tctgactatt aactcctata gttctgttga
     1741 tttgaccgaa tcaacaggct ggggcatcta ttccaacctg tatgctaata ctatcaccgt
     1801 aactaacggt ggtgtcctgg atgtgaacgt tgatcagttc gataccgaag ctttccgtac
     1861 cgataaactg gaactgacta gcggcaacat tgctgacaat aacggtaatg tagtatctgg
     1921 tgtattcgat atccatagca gcgattatgt tctgaacgct gatttggtga acgaccgtac
     1981 ctgggatact tccaagtcta actacggcta cggtattgtt gctatgaact ctgatggtca
     2041 tctgactatc aacggtaacg gtgacatgaa caacggcgac gaactggata acagctctgt
     2101 tgacaacgtt gttgctgcaa ccggtaacta caaagttcgt atcgacaacg caacaggcgc
     2161 tggcgctatc gctgattaca aagataaaga aattatctac gtaaacgacg tcaacaccaa
     2221 cgcgaccttc tctgctgcta acaaagctga cctgggtgca tacacctatc aggctgaaca
     2281 gcgcggtaac accgttgttc tgcaacagat ggagctgacc gactacgcta acatggcgct
     2341 gagcatcccg tctgcgaaca ccaatatctg gaacctggaa caagacaccg ttggtactcg
     2401 tttgaccaac tctcgtcatg gcctggctga taacggcggc gcatgggtaa gctacttcgg
     2461 tggtaacttc aacggcgaca acggcaccat caactatgat caggatgtta acggcatcat
     2521 ggtcggtgtt gataccaaaa ttgacggtaa caacgctaag tggatcgtcg gtgcggctgc
     2581 aggcttcgct aaaggtgaca tgaatgaccg ttctggtcag gtggatcaag acagccagac
     2641 tgcctacatc tactcttctg ctcacttcgc gaacaacgtc tttgttgatg gtagcttgag
     2701 ctactctcac ttcaacaacg acctgtctgc aaccatgagc aacggtactt acgttgacgg
     2761 tagcaccaac tccgacgctt ggggcttcgg tttgaaagcc ggttacgact tcaaactggg
     2821 tgatgctggt tacgtgactc cttacggcag catttctggt ctgttccagt ctggtgatga
     2881 ctaccagctg agcaacgaca tgaaagttga cggtcagtct tacgacagca tgcgttatga
     2941 actgggtgta gatgcaggtt ataccttcac ttacagcgaa gaccaggctc tgactccgta
     3001 cttcaaactg gcttacgtct acgacgactc taacaacgat aacgatgtga acggtgattc
     3061 catcgataac ggtactgaag ggtctgcggt acgtgttggt ctgggtactc agttcagctt
     3121 caccaagaac ttcagcgcct ataccgatgc taactacctc ggtggtggtg acgtagatca
     3181 agattggtcc gcgaacgtgg gtgttaaata tacctggtaa tattcttcac tccgaagaaa
     3241 ttctggtaat ttaatctaaa taatgcccgt caaggatttg acgggcatta ctgcaaagga
     3301 cgcgcaaatg ttatctgtag ttaaacctct tcaggaattt ggtaagctcg ataaatgttt
     3361 gtccagatac ggtacgcgct tcgagtttaa taatgaaaag caagttatat tttccagtga
     3421 tgtcaataac gaagatactt tcgttatttt agaggacgtt atctctctgc gtagagaaga
     3481 aaacgtactt atcggtatta cccaggctcc ttatattatg gggctggctg atggtttaat
     3541 gaaaaacgat ataccataca aattaatatc agaaggaaat tgtacgggat atcatctacc
     3601 agccaaacaa accattacgc ttattgaaca aaatcaactc tggcgagacg ctttttactg
     3661 gttagcctgg caaaatagaa ttctggaatt acgcgacgtg cagctcattg ggcataattc
     3721 ctacgaacaa atccgcgcaa cgttattatc aatgattgac tggaatgaag aattgcgatc
     3781 acgtattggt gtgatgaatt atatccatca acgtacacgc atatcgcgtt ctgtcgtcgc
     3841 agaagttctc gctgctttgc gtaaaggcgg ctatatcgaa atgaataaag gcaaactggt
     3901 cgctatcaac cgtttgcctt cagagtatta atcaggacgc ggggataacc aacggtttat
     3961 tcccgcttag ctcggtcacc aggtcattga tgccatcgct catattttta aagcgcgtca
     4021 acggcgaacg agtgaccacc acaaacgcgc caatattctt ctgcgggatc atcgccatat
     4081 aggtaatgaa accgccacca ccgcctgtct tctgaataat ccccggacga ccctctttcg
     4141 gggccatata tacccagcct aaaccaagcg catccgcttt gccggggaca tccatgccaa
     4201 tcactttcgt aaactgcgcg cgctgataaa tcaacgtctg catgcgatct gcctggttac
     4261 tgcgctgata aaaatcagat gacaaatact gctgcatcca gcgcatcata tcgccaggcg
     4321 tggaatagac gccgccgctg ccaattgccg ccagcgtgtt attgcatgga ctggcaccgc
     4381 gctctgcaac cattaaacgg cggcactgat ccggtgaggg ggtgtaggtg gtgtctttca
     4441 tccccaacgg acgggtaatc tgctcttcaa acaactgggt ataaggcttg ccggacgcat
     4501 tcgccagtgc atctgccagc aggtcgaacg caaggttaga gtacgccgcc tgactgcctg
     4561 gcgcggcttt cagcttcgcc gtagaaaggt atttccagcg ttgctcgcgc gttggccaga
     4621 caaaaaccgg acgatgtgcc gcgccaccgg gctgttcacg gggcagggcg ctggtatggg
     4681 ttgccagatt gaccagcgta atgggcgttc cattgtaggt tggcacgcgt gcgcctggcg
     4741 gagcatattt acttagcgga tcgtttaact tcacggtccc ctggtcgagc aatttcacca
     4801 gcatctcact ggtcattagc ttggtgaggg aagcaatacg cacgaccgaa tccagctgcg
     4861 ggcgaacgtt attaccaggt cgcgtttcgc cataactgcg aaagacgcgc tggttaccgt
     4921 cgataacaac cagcgccatc cccgtagcac cgctgccgta aaaaatatga tcggcataac
     4981 gatcaacaat atcagaagcg aactccggtt cagtgatcgg ctgtgccgca tggacggagg
     5041 tcaatgacgc cgcacacagc acggcagaaa aaaacagact acgtttcaac ggtggtgtcc
     5101 ataaattgaa tgaaggtaac tgagcgtatt tatactactc attgatggat tgcaaagggc
     5161 gaattagtgc aataatagtg ttaaaaacgt aacggcgcgt aaacacgatc tttttcctgc
     5221 tcaccaatgc ctgcatttgt gatcctgcgc gtgttaatcc gcttatgtaa acaatctttg
     5281 gtttgtgaaa tccgtcgaaa caattcttat ggtcaggctg gcgaactaag cgccttgcta
     5341 tgggtcacaa tgggcgattt catttggctg acgctttgta gcggtcatgc ggttaataca
     5401 cagcagttgt taaaacgata agaagttagc aggagtgcat atgtttaagt cttttttccc
     5461 aaagccggga acgttttttc tctcggcctt cgtttgggca ttgattgccg ttatcttctg
     5521 gcaagccggt gggggtgact gggtggcgcg tatcaccggc gcttccgggc agatcccgat
     5581 tagcgccgcg cgtttctggt cgttggattt cctgattttt tacgcttact acattgtttg
     5641 cgtaggactt tttgcattgt tctggtttat ctacagcccg caccgttggc aatactggtc
     5701 aatactcggt actgcactga tcatcttcgt cacctggttt ttggtggaag tcggggttgc
     5761 cgtcaacgcc tggtatgcac cgttctatga tctgattcaa accgcgctaa gttcgccgca
     5821 taaagtcact atcgaacaat tttaccgcga agtgggcgtc tttctgggga ttgcgctgat
     5881 cgcggtagtg atcagtgtgc tgaataactt ctttgtcagt cactacgtgt tccgctggcg
     5941 tacggcgatg aacgaatatt acatggcgaa ctggcaacaa ctgcgtcata tcgaaggggc
     6001 cgcacagcgt gtgcaggaag acaccatgcg ttttgcttca acgctggaga atatgggtgt
     6061 cagttttatc aacgccatca tgacgttgat cgccttcctg ccggtgctgg taacgctctc
     6121 cgcgcatgtg ccggagctgc cgattatcgg gcacattccg tatggtctgg tgattgccgc
     6181 tatcgtctgg tcgctgatgg ggaccggatt gctggcagtg gtagggatca aactgccggg
     6241 gctggagttt aaaaaccagc gagtagaggc tgcctaccgt aaagagctgg tttatggtga
     6301 agacgatgcc acgcgcgcga cgccgcctac ggtacgcgag ctgtttagcg ccgtacggaa
     6361 aaactatttc cgcctctatt ttcactatat gtatttcaac atcgcccgca ttctctattt
     6421 gcaggtcgat aacgttttcg gtttgttctt gctgtttccg tcaattgttg ccggtacgat
     6481 tacgctcggc ctgatgacgc agattaccaa cgtttttggt caggttcgcg gtgcttttca
     6541 gtacctgatt aactcatgga ccacactggt tgagttgatg tctatctaca aacgtctgcg
     6601 cagctttgaa catgagctgg atggtgacaa aattcaggaa gtaacccata ccttgagcta
     6661 aaaaggagaa gtaatgtcgc gcgtcaatca tctatcttcg ctgtcattgt tggcggttct
     6721 ggttttagcg gggtgtagta gtcaggctcc gcaaccgttg aaaaagggcg aaaaagcgat
     6781 agatgtagcg agcgtcgtgc ggcagaagat gcttgcgagc gtgaaagacc gcgatgcctg
     6841 ggcgaaagat ctcgccacca cctttgaaag tcaggggctg gcaccgacgc tggaaaacgt
     6901 ctgctcggtg ctggcggtgg cgcagcagga gtcaaattat caggcagatc cggccgttcc
     6961 gggcttaagc aagatcgcct ggcaagagat tgaccgtcgt gctgaacgga tgcacattcc
     7021 tccttttctg gttcataccg cgctaaaaat caaatcgcca aacggcaaaa gttatagcga
     7081 acggttagat tcggtgcgta cagaaaagca attgagcgcg atttttgacg atctgatcag
     7141 catggtgcca atggggcaga cgctgtttgg ttcgcttaat ccggtgcgca ccggtgggcc
     7201 gatgcaggtc agcattgctt ttgccgaaca gcataccaaa gggtatccgt ggaaaatgga
     7261 cggtacagtc cgtcaggaag tctttagtcg ccgcggcggg ttgtggtttg gtacttacca
     7321 tttactgaat tatcccgcca gttatagcgc accgatatac cgttttgctg attttaacgc
     7381 tggctggtac gccagccgta atgccgcgtt tcagaacgcg gtcagtaagg ccagcggcgt
     7441 gaagctggca ctggacggtg atttaattcg ttacgacagt aaagaacccg ggaaaaccga
     7501 actggcaacg cgcaaactgg cggcaaaact ggggatgagc gacagcgaaa ttcgtcgtca
     7561 gctagagaaa ggtgacagct tctcttttga ggagacggcg ctgtacaaga aagtttatca
     7621 acttgccgaa gcgaaaacgg gtaaatcact cccccgcgaa atgttgcctg gcattcaact
     7681 ggaaagcccg aaaatcacgc gcaacctgac tacggcctgg tttgcgaagc gcgtagacga
     7741 acggcgggcg cgttgtatga aacagtgatc aaacaggaat gtcaggccag ataaggcgtt
     7801 ttaggccgta tctggcaacg taaatcttac tggcggcgat gtcgccagtg cagccatagc
     7861 gccaccactg caaaaatgag gcagcctact aaaaacggga tcagcccgaa aatggtgccg
     7921 acacctaaac caatttccac ccgtgggcga cctgtttcct ggacctgcat taaacgctca
     7981 tagacgccgg gcgcatgcac taacaggttc agcaattgcg ccccagccca aaagcaaaac
     8041 agcacaaaca acgcataggc aatgttgcca ggcgttgaag aggcattgcg atattttccg
     8101 ctaaacagcg attttgacag ggtgaaatca gccataaaaa cctccggaag tcatctgctt
     8161 ctcttctggt gtgataccag cggcttatgg caagtctgac aggtcattta gattgtcaat
     8221 atcagaatca tggtaaattg atgttgggaa tattcccgaa gcgtgaatct tcgttgcgag
     8281 tcacaaaata gcaactttaa gttaaattta ttaataattg cgaaaatgtt gtctacacca
     8341 cagacggttg ttgcagaata tgcaaggatg ttgtttttcg ttaacggagc agccatgaat
     8401 ctgcctgtaa aaatccgccg tgactggcac tactatgcgt tcgccattgg ccttatattc
     8461 attcttaatg gcgtggtggg gttactggga tttgaagcaa aaggttggca gacctatgcc
     8521 gtcggtctgg tgacgtgggt gattagtttc tggctggcgg ggttgattat tcgtcgtcgc
     8581 gatgaagaaa ctgaaaacgc ccaataagaa tattatcggg cgttaaaata ttacattgtg
     8641 gttttcaatg cgttatccgc agcgtgacgc tccagcgcca gctcaatcaa acgtgtgatc
     8701 agatcggtgt aacccagacc gctggcttgc cacagcttcg gatacatact gatgttggtg
     8761 aagccaggca gtgtgttgat ctcgttgatc accacttcgt tctctggggt taaaaacacg
     8821 tctacacgcg ccatgcctgc gcatcccaac gtctgataag cctgaacggc aatcgcccgg
     8881 atcttatcgt tgatttctgg cgcaatggct gccggaacta ccactttcgc gccatcttcg
     8941 tcaatgtact tggtgtcgta ggcatagaaa tcgctggtga gtacgatctc gccacaggtg
     9001 ctggcttgcg gattgtcgtt gcccagaact gcgcattcga tctcacgacc tttgatccct
     9061 tgctcaacga tcactttatg atcgaactcg aacgccagat cgacggcaat tgcgtactgt
     9121 tcttcactgg ttactttgct gacaccaaca gaagagccct gattagccgg ttttacaaac
     9181 agtggtaacc ccagtttaga ctccacttcg gcaaaactga tgttgtgacg attagcgcgc
     9241 gtcagggtaa taaatggcgc aatgttcagc ccggcatcgc gtagcagacg tttggtgaca
     9301 tctttatcca tacaggctgc tgaagccaga acatcagaac ctacaaacgg taaattggcg
     9361 acccgcagca ttccctgcaa ggaaccatct tcgcccagcg taccgtggac aatcgggaaa
     9421 atgacatcca ccgtcggcaa cggctgaccg ttttgcgcgt cgataagctg atgctcatgt
     9481 ttacctggca cctgggcgag gctggtcgcc gaagggcgca acgcaatatg ggcaggatcg
     9541 tctgcattta gcagataatt gctggcatcg ctgacgtgcc attgcccttg tttatcaatg
     9601 cccagcagca caacgtcgaa gcgactttta tcaatggcat cgacaatgtt ttttgccgat
     9661 tgcagagaca cttcatgttc cgctgattta ccaccaaaaa cgattcctac ccgcagtttt
     9721 tccatcttaa aaacctatcc cgtctaacac aaagtgcata cattaccacg acaaaacggg
     9781 ggattcgcgg ccttctgaaa gattgttgca atcttctgct gacaaagcgt gcaacgtact
     9841 ggtgaagaaa gtgcgttatc tcaaagatgt gcgcaagatc acaaaaatga tgaacgggaa
     9901 gctaatttat tcctggctta aatggccatg cggtga
//
LOCUS       DAPGOF010000181         9898 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.182, whole genome shotgun sequence.
ACCESSION   DAPGOF010000181 DAPGOF010000000
VERSION     DAPGOF010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9898
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.182"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1..76
                     /locus_tag="RHV13_003961"
     tRNA            1..76
                     /locus_tag="RHV13_003961"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34..36,aa:Thr,seq:ggt)
     gene            116..231
                     /gene="rrf"
                     /locus_tag="RHV13_003962"
     rRNA            116..231
                     /gene="rrf"
                     /locus_tag="RHV13_003962"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(294..1052)
                     /locus_tag="RHV13_003963"
     CDS             complement(294..1052)
                     /locus_tag="RHV13_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDV1243438.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEDLAVHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1060..2163)
                     /locus_tag="RHV13_003964"
     CDS             complement(1060..2163)
                     /locus_tag="RHV13_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDV1243439.1"
                     /translation="MTKVLLSHPPRPASHNSNRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPLLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFALERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2173..3354)
                     /locus_tag="RHV13_003965"
     CDS             complement(2173..3354)
                     /locus_tag="RHV13_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDV1243440.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSVLCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLVFLSNRGLYIPSPQLGDGFLAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3422..4447)
                     /locus_tag="RHV13_003966"
     CDS             complement(3422..4447)
                     /locus_tag="RHV13_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV1243441.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(4878..5099)
                     /locus_tag="RHV13_003967"
     CDS             complement(4878..5099)
                     /locus_tag="RHV13_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243442.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            complement(5353..8457)
                     /gene="acrF"
                     /locus_tag="RHV13_003968"
     CDS             complement(5353..8457)
                     /gene="acrF"
                     /locus_tag="RHV13_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HDV1243443.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPDTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
     gene            complement(8469..9626)
                     /gene="acrE"
                     /locus_tag="RHV13_003969"
     CDS             complement(8469..9626)
                     /gene="acrE"
                     /locus_tag="RHV13_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HDV1243444.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEVR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
BASE COUNT         2637 a         2545 c         2379 g         2337 t
ORIGIN      
        1 gctgatatgg ctcagttggt agagcgcacc cttggtaagg gtgaggtccc cagttcgact
       61 ctgggtatca gcaccacttt ttaggttaaa gttcggcaga ttagaaaaga atttgtctgg
      121 cggcagtagc gcggtggtcc cacctgaccc catgccgaac tcagaagtga aacgccgtag
      181 cgccgatggt agtgtggggg ctccccatgc gagagtaggg aactgccaga catcaaatca
      241 agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt caattaatgg
      301 attacctgcg ataaaaatgc ccttgtacgc tctgatttag gatgcgcaaa aaattcatca
      361 ggtgcagctt gctccactat ttcgccacga tccataaaaa tcacccggtc agcgacggtt
      421 cgcgcaaacc ccatctcatg ggttacacac aacattgtca tacccgattg cgccagccca
      481 atcatcgtat ccagcacctc tttcaccatc tccggatcga gcgccgatgt gggctcatca
      541 aacaacataa ttttcggctt catacacagc gagcgcgcaa tggcaacgcg ttgctgctga
      601 ccacctgaaa tctgtccggg aaacttatgc gcatgttcgg caattctcac ccgctcgagg
      661 taatgcaccg ccagatcttc agcctctttc ttaggcatct tgcgtaccca aatcggtgcc
      721 agggtacagt tctgtagaac ggtcagatga gggaagagat tgaaatgctg aaagaccatt
      781 cccacttcct gcctgacgcg ctcaatattg cggatatctt cattaagttc gatgccatct
      841 accacgatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat ggttgtcgat
      901 ttaccggaac ctgaagggcc acacagaacg atccgttctc ccggttgcac ggttaaattt
      961 atatttttca aaacatggaa ttgtccatac catttattga cgttttccag cgtaatcatc
     1021 gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg tacgcccggt
     1081 gttaaaacgt ttttccagat gctggctata gcgcgacatg ctgaaacaga agatccagta
     1141 gatcagcgcg gcgaaaacat acccttccgt cgacataccc aaccaggcgg gatcaacggt
     1201 tgcctgctga acgctactga aaagatcgaa caacccgata atgatcacca ggctggtatc
     1261 tttgaagagt gcgatgatgg tatttaccag cccaggaatt accagcttca acgcctgtgg
     1321 cagaataacc agcccctgag ttttccagta acccaacgcc agcgactctg ccgcttcata
     1381 ttgcccttta ggcagcgcct gtaatccacc tcgcacgact tccgcaacat atgctgactg
     1441 aaacaggatc acgccaacca gcgcgcggat caatttgtcg atactggtgc cttctgccat
     1501 aaacaacggc agcatgaccg aagacataaa cagaacggta atcagcggca cgccgcgcca
     1561 gaattcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc ggcgacctaa
     1621 cgccagtaag ataccccacg gtagcgcccc cgcaatccca acggatgcaa taattaaagt
     1681 tagcgtcagc ccgccccatt gccgggtttc aacccgctca agagcaaaaa aaccgccata
     1741 catcagccac cagacaatca gtgggtaaat caccgcccag gccgcaatat agcgaccgcg
     1801 atgcgggagt attttccaga acattggtgc gatcgacaca agcccaatca gtaatgcgag
     1861 gttaattcgc cagcgttggt cgtgtgggta aagcccatac ataaactgac caaatcgttc
     1921 gtggatgaag acccaacagg caccggcttt tgtgcagtcg gcacgcgttg agccaaccca
     1981 gttagcctgc aaaaatgccc agttcagcaa cagtgggatc agttcccaca tcaaccatat
     2041 gcagccaata gtcagcaggc tattgctcca gctggagaac agattttttc gcacccacac
     2101 catcgcgcgg tttgagttat ggctcgccgg gcgcggggga tgagacagca atacttttgt
     2161 catcatgact ccttagcgtt ccacgatcgc gatgcggcgg ttatagatat tcatcagcag
     2221 cgagatagtc aggctgataa tcagatagac cgacatggtc atggctatcg tctcgatggc
     2281 ttgccccgtc tgattcagca cggtgccggc aaacagcgaa accatatcgg gatagccaat
     2341 agcggcggca agagaggagt ttttgacgat gttgagatac tggctggtta acggtggaat
     2401 aatcacccgc aatgcctggg gaataatgac ctggcgtagc gtaaccgggt tgggtaatcc
     2461 cagtgaccga gccgcttcat gttgaccata aggcactgcc tggatcccag cgcggataat
     2521 ctcggcgata aatgcagagg tataaaccga aagtgccagc gttaaggctg ccagttcagg
     2581 aattaaaacc atcccgccgc ggaagttaaa gccttgtagg gctgggacat cccagtgtag
     2641 tgctgcgcca aaaagccatt gcgccagtaa aggcaaaccg atgatcaaca ctgcggcgat
     2701 gggccaagtg cggcgcagct gtccggtctt tatctggtat gttttattaa agcggaatag
     2761 cccaacagaa aggactatag ccataacaac agccaggata aacgcaagaa atccgtctcc
     2821 cagctgcggt gacggaatat aaagcccgcg attgctcaaa aagacgagat caaacgcact
     2881 cactgcctgg cgtggtccgg gcaaattgcg caacacggca aagtaccaga agaagatttg
     2941 tagcagcggc ggaatattac ggaagatctc aatataaatt gtggaaagct ttcgtagcag
     3001 ccagttatcc gaaagtctcg ccagaccgat aaagaagccc agaacagaag cgaacacaat
     3061 acacaataca gaaaccagta gcgtattgag taagccgaca ataaaaacgc gtccgtaggt
     3121 gtcgccctgc tggtaatcga tcaaatgctg gacaataccg aagccagcgc cgcgatccag
     3181 aaaggcaaaa cctgaagtaa tgccacgatt attgagattc gttacagtgt tgtgaaataa
     3241 ccaaccaaca atgccgacaa cagcaacaac ggcaaggatc tggaataacc aggcacgaac
     3301 cgtagggttg gcaaaggaga gtgagccttt aacggttgag cggcgatgag acatagaaac
     3361 ctcggaaagc ataacgctga agagagcgcc gccgtagcga cgctcgttac ctcacaccga
     3421 cttaacgcac gggcggcgcg tactgaatac cgccgttatt ccagagatta ttttgcccac
     3481 gtttaatttt cagcgggctt tctgaaccta cgttacgctc aaaaatttcc gagtagttac
     3541 ccacctgttt gatgatgtta taggcccatt tattatccaa cttcagatcc ttgccgtaat
     3601 cgccttcttt acccagcaga tgtgccatat caggcgttgc tggattagcc gctttttcat
     3661 cgacgttctg ggaattgatg cccatctctt cagcatttag catggcgaaa agcgtccagc
     3721 gtacaatcga gaaccattca tcatcgccac gacgaactac cggcccaagc ggttctttag
     3781 agataacctc cggtaagaca atccattcag ccgggttgct taatttgatg cgcagggcat
     3841 acagttggga ttgatccgaa gccagcgtat cgcagcgacc agattccagt gccttcgctg
     3901 attcgtcaga gcgatcgaaa gtcactggtg tgtacttcat attgtttgcc ttgaaatagt
     3961 cggcgacgtt gagttcggta tcagtacccg cctgaataca gacggtagcg ccatccagtt
     4021 ctttcgcgct ttttagcccc gctttatcgt gcgtcaggaa gccaatgccg tcgtaataag
     4081 tgacgccagt aaatgccatt cccatcccgg catcgcgaga tgaagtccag gtcgtattac
     4141 gggagagcaa atccacctcc cctgactgta aagcggtgaa gcgttctttt gctgtgagcg
     4201 gggtatattt cactttcgta tcgtcaccga atacagccgc tgcaacacca cgacaaacat
     4261 ctacatcaat acctgaaaac ttaccgtcag catcggcata agagaaccca ggtaatccat
     4321 cactgatccc gcattgcaca aaaccttttt tctgaacggc atcaagcgtc gcgccagcat
     4381 gcgcctgatt tgcaacggca agcagcacgc tggcggcagc cagtgtggct atcatcatct
     4441 ttttcataat gcttcctgtg aggcgaaaat tatcgttata ggacgtttgg gaaacgtctt
     4501 ttacctgtct gtggccatcc tcttataagc aaaagtagtg ccatgttttg cgcacgattt
     4561 cgcgtcgcaa taagctgagt gtagacagga aataataaaa acagcgcccc gcaatggtgc
     4621 gaaaatgaaa catgcgccac attttggcgc aaaatattta cgcgtgtttt actaagcaga
     4681 ataatgattg ttagcgtgag tatatttctg gtgggaagtg cgaaagttaa attgatgcgt
     4741 tatttcgaaa gcagcgaaat aacgcgatat aacatgctaa atcagcagag aattagccag
     4801 cagaatatag caatatttta tcttatgtaa agatactgta tcgttaaaca gagcgcgaaa
     4861 ctccgcgccc tgttcagtta tttcgacaga gcgatgatcc ccatcgtcag gccagcaaca
     4921 gctgctgctc cgccagcaat agccccagcc gtttcccagt tatcctgagt tgtgcctttc
     4981 atacaggtat tggtgtgaac cagacgcccc tgatcgtcat aaaccggaac gcagggcgaa
     5041 tcgtgagcac agcccgccag caatgcggtg agcaatgcca caggaattaa tcttttcatg
     5101 gtgttacctc agtaataaac gctaacgctt aataagttaa ctctcttatt aacgagctga
     5161 atgccatttt atcaccggag gttataaatc attgcggaat gaataatgac aaattatgtg
     5221 aattgtaaaa atagtgaagg gagcgggaac tatagaaagc ttttaaagag tgagcaaaag
     5281 aatatcttat taatttttat gggtttcact gaaaataata aaggcacccg aaagcgcctt
     5341 tatgtttcag atttatcctt taaagcaacg gcggatcacc acaaagaaca ccggtacgaa
     5401 gaagattgcc agcaacgttg cagagaccat tcctcccatt accccgatac ccactgcgtt
     5461 ctgcgcgcca ctgccggcac cgttactgat agccagcggt aatacgccga gaataaaggc
     5521 gagagaggtc atcaggatag gacgcagacg catacgtact gccattagtg tcgcttcaac
     5581 gacaccttta ccctctttct ccatgagatc tttagcgaac tcaacaatca aaatagcgtt
     5641 tttggccgac aagccaattg tcgttagcaa gcccaccata aagtagacgt catttttttg
     5701 attaaagagt gtcgccgcca gcagcacgcc gacaatccct aacggcacga ccaacataac
     5761 cgagacagga attgaccagc tttcatagag tgcagcaagg cacaggaaaa caaccacaaa
     5821 ggaaattgct accagagcgg gagcctggtt tcccgataag cgttcctgat acgacatacc
     5881 cgtccagtca taaccaatgc ccgcaggtaa ttttgacgca aggttttcca tcaacgccat
     5941 ggcatcgccg gaactggttc ctggcgcggc ttccccctga atctccattg acggcagacc
     6001 gttgtagcgt tccagtcgcg gagagccata cacccaatgt gaagtggtaa aggcagagaa
     6061 tggcaccatt tcgccgttgg cgctgcggac ataaagttta tcgacatctt ctggcagcat
     6121 acggaatttg gcatccgcct gaacatacac ctttttcacg cgaccacggt cgatgaagtc
     6181 gttaacgtaa gtcccaccca gcgccgttga aatggtctga ttgatgtcag aaagtgaaac
     6241 acctaatgcc tgcgcctttt cctggtcaac ttccagttta aactgcgcgg tgtcttccag
     6301 gccattagga cgcacgctga ctaagctggc aggatgttgc gccgccatgc caagcaactg
     6361 gttacgggcc tgggttaggg catcgtgacc cagcccagcc tgatcaatta actcaaagtc
     6421 gaaacccgtt gccgtgccca gttcaacaat ggctggcatg ttgaatggaa tgacaaaacc
     6481 gtcgcggatc ttgcccaatt ccattttggc acgatggatt actgcttccg cactgttttc
     6541 gtcaccatta cgctcttccc acggtttcag actgacgaag gccataccgg cgttttgtgc
     6601 ctggccgctg aagctaaagc cgttaaccgt aaagacactt tcaacgttcg ctttctcgtt
     6661 cttcagatag taatccgtca cttgatccaa cactttttgc gtccgctctt gggtcgcgcc
     6721 agcgggtaac tgaatcatgg tcagaaagac accctgatcc tcttcaggta agaaggaaga
     6781 cggaagacgt aaaaacaaca ccaccattcc tgcaacaatc agcgcataga tcagtaaata
     6841 tcgtcctgtg gaaccgagga ttttgccgac gctgttggtg tagtggttaa cgctatgatc
     6901 gaaggtggta ttaaaccagc cgaagaaacc gcccttattt tcgtgatgct cagcagagac
     6961 gggtttaagc agcgttgcac ataacgcagg ggtaagaatc aatgccacca gaacagaaag
     7021 cgccattgcc gaaacgatgg tgatagagaa ctggcgataa attgccccag tagaaccgcc
     7081 gaagaatgcc atcggaataa ataccgctga cagcaccatc gcgataccca ccagtgcgcc
     7141 ctgaatttgc gacattgatt tttccgtcgc ttcttttggc gggagcttat cctccatcat
     7201 cacgcgctcg acgttctcca ccaccactat cgcatcatcg acgagcagcc cgatggcaag
     7261 caccatcccg aacatcgtta gtgtgttgat ggagtaacca aaagcggcga ggatggcaaa
     7321 cgtccctaac aacaccacgg gtaccgcaat ggtggggatc agcgttgctc gcatattctg
     7381 caagaacaga tacatcacca ggaacaccag cataatagct tcgaacagtg tttttaccac
     7441 ttcgtgaata gaaagctgga cgaatggcgt ggtgtcataa gggtagagaa ccttcattcc
     7501 ctgcgggaag aatggctgta attccgccag ttttgcctta atggctttcg cggtatcgag
     7561 agcattcgcg ccggttgcca gcttaatacc caggcccgcc gccggttttc cgttgatgcg
     7621 agcgataacg ttatagtttt caccgccaag ttcaacccgt gcgacatctt tcagacgcac
     7681 caccgagccg tcactgttta cgcgcagggt cactttgccg aattcttccg gattttttaa
     7741 ccgcgtctga gcaataatcg aggcattcaa ctgttgccct ggtaacgctg gcgttccgcc
     7801 caactgtccg gcagcgatct gatcgttctg taccttcaac tggttaatca catcaaccgg
     7861 tgtcagttta tatttgttta gcagatcggc gtccagccag atacgcatcg catactgtgc
     7921 gccgaaaagc tgtacgtcac cgacgccatt cagacggcta agcgtatctt taacgttaga
     7981 ggccacatag tccgagatat cgtcctgtgt ggtgtctggg ttatcagaga caaagcccgc
     8041 caccatcaaa tagctgctac tggacttttc aacactgatc ccctgctgct gaacctcctg
     8101 cggcagcaac ggcgtggcga gctggagttt gttctgcacc tgcacttgcg cgatatcagg
     8161 atcggtcccg gactggaagg taagggtaat tgtcacgcta ccggcggaat cgctggtgga
     8221 ggacatatac atcaggttat cgataccgtt catattctgt tcgataacct gcgtcaccgt
     8281 atcctgcacg gtctgcgcat cagcgcccgg atagtttgct gaaacagaaa ccgcaggcgg
     8341 tgcaatggtt ggatactgag cgacgggcaa ttgtaggatc gccagtgcgc ccgccatcat
     8401 cagaataatg gccagcaccc atgcaaatat cggtcgtcga ataaaaaagt ttgccatgtc
     8461 agattacctt acttcgatgc agtatctgcg ggggtatcgg tagtggcttt aacctgctct
     8521 cccggacgcg ctttttgcag gccgctgaca atgacttgat cgccagattt cagaccttcg
     8581 ctaatcaacc atttatcgcc aatcgcctga ctggcaacga cagggcgcac ttccacctga
     8641 cttttatcgt taacaatcag cacggttgca tcaccacgcg gtgtgcggct aacgccttgt
     8701 tgcgggatga gaatggcgtc aggttggacg ccttcatcaa tccgtgcacg cacaaacata
     8761 cccggcaaaa gcgtatgttg cgggttaggg aagacagcac gtagggttat ggagccggtg
     8821 ctttcatcaa cggtcacatc ggagaattgc agcgtacctt tcaggggata ggtttgaccg
     8881 ttttccatga ccaactctac gttgctggtg gcgttttcct tatgcaaatt tccttgctct
     8941 acggattgct tcagcctcat aaaatcgttg ctggattggg tcacatcaac gtagatagga
     9001 tcgagctgct ggacagttgc cagttcagtc gtttgcccat tagtgacaag agcgccttcg
     9061 gtcacagttg atttgccgat acgtccgcta attggcgcag tcactttggt ataagcaaga
     9121 ttgatgcgag cgctttcgac tgtggctttt gcggcaatca cggcggcatc ggcctggcga
     9181 gcatcagcaa tggcctggtc gtactcctgc tggctgatgt atttcgtacc cacgagcgga
     9241 acgtaacgtt ttaccgtcaa atgcgcgatg gcggcggcgg cttcactttt cgccagttcg
     9301 cctttcgcgc tgtcataatt tgcctgatag gtcgcgggat cgatctggta cagggactgg
     9361 cctgcttgca catcgctgcc ttcagtgaaa ttacgattca gtacgatccc gctaacctgt
     9421 gggcgaactt cggctatacg gtaagcattg gtgcggcctg gtaattcagt cttaacttct
     9481 aacggggccg ttttcacaat atgaacggta acctgcggtt caccgacgtg agctttctct
     9541 tctcccttat cgttacaacc ggcgattaaa gccgcggaga tcagaataaa ggagggcagg
     9601 aggaaaaacc tggcatgttt cgtcattact attcctcaaa aaaccaaaag cgcgttattt
     9661 acccaaaaag gcaacgcgtt aactcgcaga aagaaaaata cagtttgcta tcctacaaat
     9721 tatcattcgt cgatgtaagg aatagttatg aatactggcg tctcaaggca cataaacaca
     9781 aaaaaagatt aatattctac tgttttattt tgacgcgggt tgaaagaggc agaattaaaa
     9841 cctcgtaaat tgaaatatat attgatgtag tgaatgtatc ttaggtaaat aatatata
//
LOCUS       DAPGOF010000182         9887 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.183, whole genome shotgun sequence.
ACCESSION   DAPGOF010000182 DAPGOF010000000
VERSION     DAPGOF010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.183"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..665
                     /locus_tag="RHV13_003970"
     CDS             <1..665
                     /locus_tag="RHV13_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HDV1243445.1"
                     /translation="LSVLMISPLCMLTGCDYIGKAKLIDDLSKQQELQKSKIEQLEKQ
                     QEQDKRKIELFEKQQTAIISSTKTLASVVKGVKEKQDEFVFTEFNPAQTQYFILNNGS
                     VGLAGRVLAIDAVENGSVIRISLVNLLSVPVSNIGFHATWGNERPTDAKALAKWQQLL
                     FNTALNSTLQLMPGQWQDINLTLKGVSPNNLKYLKLSINMANLQFDTVQSAETRQRKN
                     KK"
     gene            709..5805
                     /locus_tag="RHV13_003971"
     CDS             709..5805
                     /locus_tag="RHV13_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HDV1243446.1"
                     /translation="MNKIFKVIWNPATGSYSVASETAKSRGKKSGRSKLLISALVAGG
                     MFSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYEAIA
                     EGQYSSAIGSRTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASAEN
                     AIALGRSSVASGTDSLALGRQTLASAANAIAIGAESKAAENATAVGTKAEANGLNSIA
                     LGSGGIADADNTIALGNQSLAVAAGAIAIGQGNKADGANAIALGNGSVTGGANAIALG
                     QGSYAGLENGIAIGGQASAQGTNSVALGAGSVATEDNSVSVGNTTDQRKIVNMAAGDI
                     STSSTDAINGSQLYAISKSVADRLGGGATVNAQGVVTAPNYRLKNGIYGNVGDALADL
                     NNNTIQWDSVKKGYSAAHGTDATSTITNVKAGTISATSTDAVNGSQLKATNDDVEANT
                     ANIATNTSNIATNTASIATNTTNITNLTNSVGDLKDDALLWNDAKNAFSAAHDKDTTS
                     KITNVKDGDLTAGSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLTETV
                     TNLDEDALKWDKDNGVFTAAHGTDATSKITNVKDGDLTADSTDAVNGSQLKTTNDAVA
                     TNTTNIANNTSNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNATSKITN
                     VKDGDLTAGSTDAVNGSQLKTTNDAVATNTTNIATNTTNISNLTETVTNLGEDALKWD
                     KDNGVFTAAHGNNTASKITNILDGTVTATSSDAINGSQLYDLSSNIATYFGGNASVNT
                     DGVFTGPTYKIGETNYYNVGDALAAINSSFSTSLGDALLWDASVDKFSAKHGTNGDAS
                     VITDVANGDVSSTSSDAVNGSQLYTTNKYVVDALGGGAEVNADGSISAPTYTIANADY
                     DNVGDALNAIDTTLDDALLWDADAGENGAFSAAHGKDKTASVITNVANGAISAASSDA
                     INGSQLYTTNKYIADALGGDAEVNADGTITAPTYTIANAEYNNVGDALDALDDNALLW
                     DETANGGAGAYNASHDGKASIITNVADGSISEDSTDAVNGSQLNATNMMIEQNTQIIN
                     QLAGNTDATYIEENGAGINYVRTNDNGLAFNDASASGVGATAVGYNAVASGASSVAIG
                     QNSSSTVDTGIALGSSSVSSRVIAKGSRDTSVTGNGVVIGYDTTDGELLGALSIGDDG
                     KYRQIINVADGSEAHDAVTVRQLQNAIGAVATTPTKYFHANSTAEDSLAVGEDSLAMG
                     AKTVVNGNAGIGIGLNTLVLADAINGIAIGSNARANHANSIAMGNGSQTTRGAQTGYT
                     AYNMDAPQNSVGEFSVGSEDGQRQITNVAAGSADTDAVNVGQLKVTDERVAQNTQSIT
                     NLNNQVTNLDTRVTNIENGIGDIVTTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAA
                     ADNSVALGTGSVANEENTISVGSSTNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRY
                     DTKADGSIDYSNITLGGGNGGTTRISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRM
                     VEMDNKLSKTESKLSGGIASAMAMTGLPQAYTPGASMASIGGGTYNGESAVALGVSMV
                     SANGRWVYKLQGSTNSQGEYSAALGAGIQW"
     gene            6231..7886
                     /gene="lldP"
                     /locus_tag="RHV13_003972"
     CDS             6231..7886
                     /gene="lldP"
                     /locus_tag="RHV13_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HDV1243447.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYIAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7886..8662
                     /gene="lldR"
                     /locus_tag="RHV13_003973"
     CDS             7886..8662
                     /gene="lldR"
                     /locus_tag="RHV13_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HDV1243448.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8659..9849
                     /gene="lldD"
                     /locus_tag="RHV13_003974"
     CDS             8659..9849
                     /gene="lldD"
                     /locus_tag="RHV13_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HDV1243449.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKEFPAALAPMAK
                     GNAA"
BASE COUNT         2483 a         2493 c         2592 g         2319 t
ORIGIN      
        1 tattaagcgt gttaatgata tcgccactct gcatgttgac agggtgtgac tatattggaa
       61 aagcaaaact aatcgacgat ctttccaaac agcaagagtt gcaaaaaagt aaaatagagc
      121 aacttgaaaa acaacaagaa caagataaac gcaagataga actatttgaa aaacagcaaa
      181 ctgccatcat aagtagtacc aaaacgcttg ctagtgtggt gaagggggtt aaagaaaagc
      241 aggatgaatt tgtcttcaca gaatttaacc cagcacaaac acaatacttc attttaaata
      301 acggctctgt cggtttggcg ggtcgggtgc tggctattga tgcagttgaa aacggcagcg
      361 ttattcgtat ttcgctggtc aacctattaa gcgttcccgt atcaaatatt ggttttcatg
      421 ctacatgggg caatgaaagg cccaccgatg ccaaagctct ggcgaagtgg caacaattac
      481 ttttcaatac cgctctgaac tccactctgc aattaatgcc tggtcaatgg caagacatta
      541 atctgacatt aaaaggcgtt tcgcctaata acctgaagta tctgaagttg tccatcaata
      601 tggccaatct ccagttcgac actgttcaat ctgctgaaac tcggcagcga aaaaacaaaa
      661 aataaatgca gcttctgcgc ttatatcaag gaatagagaa catcaataat gaacaaaata
      721 tttaaagtca tctggaatcc ggcgacgggc agttatagcg ttgccagcga aacagcaaag
      781 agccgtggta agaaatctgg tcgcagtaag ctgttaattt ctgcactggt tgcgggtgga
      841 atgttttcgt cgtttggggc attggcgaat gccgggaatg acaacggtca gggtgttgat
      901 tacggtagtg gatcagctgg cgacggctgg gttgctatag gcaaaggggc gaaagcaaat
      961 acttttatga acaccagtgg ttccagtact gctgtgggtt atgaagctat agctgaaggc
     1021 caatatagct ctgccatcgg ttcaagaacc catgcgattg gtggtgcatc aatggcgttt
     1081 ggggttagtg caatatcaga aggcgataga agtatagcac tgggtgcctc ttcgtattca
     1141 ttgggccaat actcaatggc cctcggccgt tattcaaaag cattgggtaa attgtctatt
     1201 gctatggggg actcttccaa agcggaagga gcaaacgcca ttgcactggg gaatgccact
     1261 aaagctactg agattatgag tattgctctt ggcgacaccg ccaatgcgtc aaaagcgtat
     1321 tcaatggcgc tgggtgcaag tagcgtcgca tccgcagaaa acgcaattgc cctggggcgt
     1381 agcagtgtag ctagtggtac tgacagcctc gcattaggca gacaaacact tgccagtgca
     1441 gcgaacgcta ttgcgatagg tgctgagagc aaagctgctg aaaatgcgac ggccgttggt
     1501 accaaagcgg aggccaatgg acttaatagt atcgcattgg gctctggggg tattgcagat
     1561 gccgataaca cgattgctct gggcaatcaa agcctggcag tagctgctgg tgcaattgcc
     1621 atcggtcagg ggaataaagc tgacggcgca aatgctatcg cgctgggtaa tggtagtgtt
     1681 acaggtggcg caaatgctat tgctcttgga caaggtagtt atgccggttt agaaaatggc
     1741 attgcaattg gtggtcaagc cagtgctcaa gggacaaatt ccgttgctct gggtgcgggt
     1801 tctgtagcga ctgaagataa ttccgtctct gtgggtaaca caacagatca gcgaaaaatt
     1861 gtcaatatgg cagcaggtga tatcagcact agcagtactg atgccataaa tggatcacag
     1921 ctttatgcta tcagtaagtc agtagcggac cgtcttggtg gaggggctac cgtcaatgcg
     1981 caaggtgtcg ttactgcccc gaattacaga ctgaaaaatg gtatttatgg caatgttggc
     2041 gatgctttag cggatctaaa caataacacg atacaatggg actccgttaa aaagggatat
     2101 agtgcggcac atggtacaga tgccaccagc accatcacca acgttaaagc cggcactatt
     2161 tctgctacca gtacggatgc ggttaacggt tctcagctga aagctaccaa tgacgatgtc
     2221 gaggccaata ccgccaatat cgctactaat accagcaaca ttgccacgaa tacggcaagt
     2281 attgccacca acaccaccaa tatcaccaac ctgacgaatt ccgttggtga ccttaaggat
     2341 gatgccctgc tgtggaatga tgctaaaaat gcattcagtg cagctcatga caaggataca
     2401 accagcaaaa tcaccaacgt taaagatggc gacctgacgg ctggcagcac tgatgccgtt
     2461 aacggctctc agctgaaaac caccaacgat gctgtggcga cgaataccac caatatcgcc
     2521 aataacactt ccaatattgc cactaacacc accaacatct ctaacctgac tgagacggtg
     2581 actaaccttg atgaagatgc gctgaaatgg gataaggaca atggtgtatt cacggcagct
     2641 catggcaccg acgccaccag caaaatcacc aacgttaaag atggcgacct gacggctgac
     2701 agcaccgatg ccgttaacgg ctctcagctg aaaaccacca acgatgctgt ggcgacgaat
     2761 accaccaata tcgccaataa cacctccaat attgccacta acaccaccaa catctctaac
     2821 ctgactgaga cggtgactaa tcttggtgag gatgcgctga aatgggataa ggacaatggt
     2881 gtattcacgg cagctcatgg caacaacgcc accagcaaaa tcaccaacgt caaagatggc
     2941 gacctgacgg ctggcagcac cgatgccgtt aacggctctc agctgaagac caccaacgat
     3001 gccgtggcga cgaataccac caatatcgcc actaacacca ccaacatctc taatctgact
     3061 gagacggtga ctaatcttgg tgaggatgcg ctgaaatggg ataaggacaa tggtgtattc
     3121 acggcagctc atggcaacaa caccgccagc aaaatcacca atatcctgga cggcacagtc
     3181 actgcaacaa gttccgatgc cattaacggc agccagcttt atgacttaag cagcaatatc
     3241 gccacctact tcggcggcaa tgcttctgtg aatactgacg gtgtgtttac cggtccaacc
     3301 tacaaaatcg gtgaaacaaa ttattataac gtcggcgatg cactggctgc gattaactcc
     3361 tcatttagca cgtctctcgg cgatgccctg ctttgggacg cttccgtaga taaatttagt
     3421 gccaaacacg gtactaatgg tgacgcaagc gtgatcactg atgtcgcaaa cggggatgtt
     3481 tcaagcacaa gctctgatgc tgtgaacggc tcgcaactct ataccaccaa taagtacgtc
     3541 gtagatgcgc tgggtggtgg tgccgaagtc aatgctgacg gcagcatcag tgcgccgact
     3601 tacactatcg caaacgcaga ttacgataat gtcggtgatg ccctgaatgc tatcgatacc
     3661 actcttgatg acgctctgct ctgggatgca gacgccggtg aaaatggtgc atttagcgcc
     3721 gctcacggaa aagataaaac tgccagtgta atcactaacg tcgctaacgg tgcaatctct
     3781 gctgccagca gcgacgcgat taacggctca caactctata ccaccaataa gtacatcgct
     3841 gatgcgctgg gtggtgacgc agaagtcaac gctgacggca ccatcaccgc accgacttac
     3901 accattgcga acgccgagta caacaacgtc ggtgacgctc tggatgcgct tgatgataac
     3961 gccctgctgt gggatgagac tgccaacggc ggtgctggag cctacaatgc cagccatgac
     4021 ggtaaagcca gcatcatcac taatgtcgct gatggcagta ttagtgagga cagcaccgat
     4081 gcagtgaacg gttctcagtt gaatgcgacg aatatgatga ttgagcagaa cacccaaatc
     4141 atcaatcagc ttgctggtaa caccgacgca acctatatcg aagaaaatgg cgctggtatc
     4201 aactacgttc gtactaacga caacggttta gcctttaacg atgcaagcgc ctctggtgtc
     4261 ggcgctacag ctgtaggtta taacgctgtc gcgtctggtg ccagcagcgt agccattggt
     4321 cagaacagca gcagcaccgt tgataccggt attgcgctgg gtagcagctc cgtttccagc
     4381 cgtgtgattg ccaaaggttc tcgtgacact agcgtaacgg gaaatggcgt ggtgattggt
     4441 tatgacacca ctgacggcga actgctaggt gcattgtcaa ttggtgatga cggtaaatac
     4501 cgccaaatca tcaacgtagc tgatggttca gaagcccatg acgccgttac ggttcgccag
     4561 ttgcagaacg ctattggagc ggtcgccact acgccaacca agtactttca cgccaactca
     4621 acggcagaag actcactggc cgttggtgaa gactcgctgg caatgggggc gaaaactgtc
     4681 gttaatggta atgcaggtat tggtatcggt ttgaacactc tggttctggc tgatgcgatc
     4741 aacggtattg ctatcggcag taacgcacgc gcaaaccatg caaacagtat tgcaatgggt
     4801 aacggttctc agaccacccg tggtgcacag actggctaca ccgcctacaa catggacgca
     4861 ccgcagaact ctgtaggtga gttttctgtt ggtagtgaag acggtcaacg tcagatcacc
     4921 aacgtcgcag ctggttcagc ggataccgat gcggttaacg tgggtcagtt gaaagtcact
     4981 gatgagcgcg tagcgcaaaa tacccagagc attactaacc tgaacaatca ggtcactaat
     5041 ctggatactc gcgttactaa tatcgaaaac ggtattggcg atatcgtgac caccggtagc
     5101 accaagtact tcaagaccaa caccgatggc gtagatgcca acgcccaggg taaagatagc
     5161 gttgcgattg gttctggttc cattgctgcc gctgacaaca gcgtcgcact gggtacaggc
     5221 tccgtggcca acgaagaaaa cacgatctct gtgggttctt ctaccaacca gcgccgtatc
     5281 accaacgtag ctgccggtgt taatgccacc gatgcggtta acgtgtcgca gctgaaatct
     5341 tctgaagcag gcggcgttcg ctacgacacc aaagctgatg gctctatcga ctacagcaac
     5401 atcactcttg gtggtggcaa cggaggtacg actcgcatca gcaacgtttc cgcaggtgtg
     5461 aacaacaacg acgcggtgaa ctacgcgcag ttgaagcaaa gcgtgcagga aacaaagcaa
     5521 tacaccgatc agcgcatggt tgagatggat aacaaactgt ctaaaactga aagcaagttg
     5581 agcgggggta tcgcttctgc aatggcaatg accggtctgc cgcaggctta cactccaggt
     5641 gccagcatgg cttctattgg tggcggtact tacaacggtg aatcggcagt tgctttaggt
     5701 gtatcgatgg tgagcgccaa tggtcgttgg gtctacaaat tacaaggtag taccaatagc
     5761 cagggtgaat actccgccgc actcggtgcc ggtattcagt ggtaatgatc cattaagtta
     5821 gtgtgactaa gggcaaacgc caaattgcct gatgcgctac gcttatcagg cctacagggt
     5881 ggttctgcac attcctatag gccgggtaag gtgttcacgc cgcatctggc aagataaggc
     5941 gctccgggtc aacaatctaa gggcaattct ctgatgagga ttgccctttt ctttaccaga
     6001 catctccccc tcaagaattg gccctaccaa ttcttcgctt atctgacctc tggttcacaa
     6061 ttccccaatt aaaactcaca tcaatgttgc caatacataa catttagtta accattcatt
     6121 gtcattatcc ctacacaaca caattggcag tgccactttt acacaacgtg tgacaaggag
     6181 atgagcaaca gactcattac acgatgtgcg tggactccag gagacctgca atgaatctct
     6241 ggcaacaaaa ctacgatccc gccgggaata tctggctttc cagcctgata gcatcgcttc
     6301 ccatcctgtt tttcttcttt gcgctgatta agctcaaact gaaaggatac atcgctgcct
     6361 catggacagt ggcaatcgcc cttgccgtgg ctttgctgtt ctataaaatg ccggtcgcta
     6421 acgcgctggc ctcggtggtt tatggcttct tctatggttt gtggcccatc gcgtggatca
     6481 ttattgcagc agtgttcgtc tataagatct cggtgaaaac cgggcagttt gacatcattc
     6541 gctcgtctat tctttcgata accccagacc agcgcctgca aatgctgatc gtcggtttct
     6601 gtttcggcgc gttcctcgaa ggagctgcag gctttggcgc accggtagca attaccgccg
     6661 cattgctggt cggtctgggc tttaaaccac tgtacgccgc cgggctgtgc ctgattgtta
     6721 acaccgcgcc agtggcattt ggcgcgatgg gcattccaat cctggttgcc ggacaggtaa
     6781 caggcatcga cagctttgag attggccaga tggtggggcg tcagctaccg tttatgacca
     6841 taatcgtgct gttctggatc atggcgatta tggacggctg gcgcggtatc aaagaaacct
     6901 ggcctgcggt cgtggttgcg ggcggctcgt ttgccatcgc tcagtacctt agctctaact
     6961 tcattgggcc ggagctgccg gacattatct cttcgctggt atcgctgctc tgcctgacgc
     7021 tattcctcaa acgctggcag ccggtacgcg ttttccgctt cggcgatctg ggtgcatcgc
     7081 aggttgatat gacgctggcc cacaccggtt acacggcggg tcaggtgctg cgtgcctgga
     7141 caccgttcct gttcctgacc gctaccgtaa cactgtggag tatcccgccg tttaaagccc
     7201 tgttcgcttc tggcggcgcg ctttatgaat gggtgatcaa cattccggtg ccgtacctcg
     7261 ataaacttgt tgcccgtatg ccgccggtcg tcagcgaagc taccgcttac gccgccgtgt
     7321 ttaagtttga ctggttctct gccactggca ccgccattct gtttgctgca ctgctctcaa
     7381 ttgtttggct gaagatgaaa ccgtctgacg ctatcagcac cttcggcagc acactgaaag
     7441 aactggccct gcccatctac tctataggta tggtgctggc gttcgccttt atctctaact
     7501 attccgggct gtcatcaaca ctggcgctgg cactggcgca cacaggtcat gcttttacct
     7561 tcttctcacc gttccttggt tggctggggg tattcctgac cgggtcggat acgtcatcta
     7621 acgctctgtt tgctgctctg caagccactg ccgcacaaca aattggcgtc tctgacctgt
     7681 tgctggttgc agccaacacc accggtggcg tcaccggtaa gatgatctcc ccgcaatcta
     7741 tcgctatcgc ctgtgcggcg gtaggcctgg tgggcaaaga gtctgatttg ttccgcttta
     7801 ctgtcaaaca cagcctgatc ttcacctgta tggtgggcgt gatcaccacg cttcaggcct
     7861 atgtcttaac gtggatgatt ccttaatgat tgttttaccc agacgcctgt cagacgaggt
     7921 tgccgatcgt gtgcgggcgc tgattgatga aaaaaacctg gaagcgggca tgaagttgcc
     7981 cgctgagcgc caactggcga tgcaactcgg cgtatcacgt aattcactgc gcgaggcgct
     8041 ggcaaaactg gtgagcgaag gcgtgctgct cagtcgacgc ggcggcggga cgtttattcg
     8101 ctggcgtcat gacacatggt cggagcaaaa catcgtccag ccgctaaaaa cgctgatggc
     8161 cgatgatccg gattacagtt tcgatattct ggaagcccgc tatgccattg aagccagcac
     8221 cgcatggcat gcggcaatgc gcgccacacc aggcgacaaa gaaaagattc agctttgctt
     8281 tgaagcaacg ctcagcgaag acccggatat cgcctcacaa gcggacgttc gttttcatct
     8341 ggcgattgcc gaagcctcac ataacatcgt gctgctgcaa accatgcgcg gtttcttcga
     8401 tgtcctgcaa tcctcagtga agcatagccg tcagcggatg tatctggtgc caccggtttt
     8461 ttcacaactg accgaacaac atcaggctgt cattgacgcc atttttgccg gtgatgctga
     8521 cggggcgcgt aaagcaatga tggcgcacct tagttttgtt cacaccacca tgaaacgatt
     8581 cgatgaagat caggctcgcc acgcacggat tacccgcctg cccggtgagc ataatgagca
     8641 ttcgagggag aaaaacgcat gattatttcc gcagccagcg attatcgcgc cgcagcgcaa
     8701 cgcattctgc cgccgttcct gttccactat atggatgggg gtgcatattc tgaatacacg
     8761 ctgcgccgca acgtggaaga tttgtcagaa gtggcgctgc gccagcgtat tctgaaaaac
     8821 atgtccgact taagcctgga aacgacgctg tttaatgaga aattgtcgat gccggtagca
     8881 ctggcaccgg tgggtttgtg tggcatgtat gcgcgtcgtg gcgaagttca ggctgcaaaa
     8941 gcggcggatg cgcatggcat tccgtttact ctctcgacag tttccgtttg cccgattgaa
     9001 gaagtcgcgc cagccatcaa gcgcccaatg tggttccagc tttatgtact gcgcgatcgc
     9061 ggctttatgc gtaacgcgct ggagcgagca aaagcagcgg gttgttcgac gctggttttc
     9121 accgtggata tgccgacacc gggagcgcgt tatcgtgatg cgcattctgg aatgagcggc
     9181 ccgaacgcgg caatgcgccg ctatttgcag gcggtgacgc atccgcaatg ggcgtgggat
     9241 gtgggcctga acggtcgtcc acatgattta ggtaatatct cagcttatct cggcaaaccg
     9301 accggactgg aagattacat cggctggctg gggaataact tcgatccgtc catctcatgg
     9361 aaagaccttg agtggatccg cgatttctgg gatggcccga tggtgatcaa agggatcctc
     9421 gatccggaag atgcgcgcga tgcagtacgt tttggtgctg atgggattgt agtttctaac
     9481 cacggtggcc gccagctgga cggtgtactc tcttccgctc gtgcgttgcc cgctattgca
     9541 gatgcggtga aaggtgatat agccattctg gcggatagcg gaattcgtaa cgggcttgat
     9601 gtcgtgcgta tgattgcgct cggtgccgac accgtactgc tgggtcgtgc tttcctgtat
     9661 gcgctggcaa cagcgggcca ggcgggtgta gctaacctgc taaatctgat cgaaaaagag
     9721 atgaaagtgg cgatgacgct gactggcgcg aaatcgatca gcgaaattac gcaagattcg
     9781 ctggtgcagg ggctgggtaa agagtttcct gcagcactgg ctcccatggc gaaagggaat
     9841 gcggcatagt cgtttgcgcc ccctcaccct aaccctctcc ctcaggg
//
LOCUS       DAPGOF010000183         9842 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.184, whole genome shotgun sequence.
ACCESSION   DAPGOF010000183 DAPGOF010000000
VERSION     DAPGOF010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.184"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            149..475
                     /locus_tag="RHV13_003975"
     CDS             149..475
                     /locus_tag="RHV13_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HDV1243450.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            497..1744
                     /locus_tag="RHV13_003976"
     CDS             497..1744
                     /locus_tag="RHV13_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDV1243451.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPADIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            1759..2844
                     /gene="ypdF"
                     /locus_tag="RHV13_003977"
     CDS             1759..2844
                     /gene="ypdF"
                     /locus_tag="RHV13_003977"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDV1243452.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVLISR
                     ESAHILVDSRYYADVEARTQGYQLHLLDATHTLATIVNKIIADEHLQTLGFEGQQVSW
                     ETAHRWQSELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDDAARRV
                     ITEAGFGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            2844..3881
                     /gene="ypdE"
                     /locus_tag="RHV13_003978"
     CDS             2844..3881
                     /gene="ypdE"
                     /locus_tag="RHV13_003978"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDV1243453.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHSAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            3906..6401
                     /gene="ptsP"
                     /locus_tag="RHV13_003979"
     CDS             3906..6401
                     /gene="ptsP"
                     /locus_tag="RHV13_003979"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDV1243454.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQAFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(6404..7261)
                     /locus_tag="RHV13_003980"
     CDS             complement(6404..7261)
                     /locus_tag="RHV13_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV1243455.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRLVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(7274..8008)
                     /gene="ypdB"
                     /locus_tag="RHV13_003981"
     CDS             complement(7274..8008)
                     /gene="ypdB"
                     /locus_tag="RHV13_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HDV1243456.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            complement(8023..9720)
                     /gene="ypdA"
                     /locus_tag="RHV13_003982"
     CDS             complement(8023..9720)
                     /gene="ypdA"
                     /locus_tag="RHV13_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HDV1243457.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
BASE COUNT         2285 a         2565 c         2712 g         2280 t
ORIGIN      
        1 tttgtgtccc ctcacaaggc cgacctgcgt cacacttccg tacagcggga ttaattctcc
       61 agtaaatgca ttatttgtct ggtaacggcg atttgttttg cacgttcata atttcactcg
      121 tcaactatca ctgaacgagg aattaacgat gagtaagaaa ctgattgcct tatgtgcctg
      181 cccgatgggc ctggctcaca cctttatggc cgctcaggcg ctggaagaag cagcggtaga
      241 agccggttat gaagtgaaaa ttgaaaccca gggcgcggac ggtatccaga atcgcctgac
      301 ggcgcaggat atcgccgaag cgaccatcat catccactcc gtggcagtaa ccccggaaga
      361 taacgaacgt ttcgaatcac gcgatgttta tgaaatcact ttgcaggacg caattaaaaa
      421 cgctgcgggc atcatcaaag aaatcgaaga gatgattgcc tctgaacaac agtaatattc
      481 gacagggata attgttatgg ccattaaaaa acgcagtgca accgttgtgc ctggcgcatc
      541 cggtgcggca gcggcagtta agaatccgca ggcctctaaa agcagcttct ggggtgaact
      601 cccgcagcat gtgatgtcag ggatttcacg catggtgccg accttaatta tgggcggtgt
      661 gatcctcgct ttcagccagt tgattgctta tagctggctt aaaattcctg ctgatatcgg
      721 catcatggat gctcttaata gcgggaaatt ctccggtttc gacctctcct tactgaaatt
      781 tgcctggttg tcgcagtcct tcggcggcgt gttgtttggt tttgccattc cgatgtttgc
      841 tgcttttgtg gcgaactcta tcggtggcaa actggcgttt ccggctggtt ttatcggcgg
      901 cttgatgtct acccagccga cgcaactgct gaacttcgat cccagcacta tgcaatgggc
      961 gacctcttcg ccggtgccgt ccaccttcat tggtgcgctg attatttcta tcgtcgcggg
     1021 ttaccttgtg aagtggatga accagaaaat ccagttaccg gatttcctgt tagcgttcaa
     1081 aaccacattt ttgctcccga ttctttccgc catttttgtc atgctggcga tgtactacgt
     1141 catcaccccc tttggtggct ggatcaacgg cggtatccgt actgtgctga ctgccgcagg
     1201 tgagaagggc gcgctgatgt acgcaatggg gatcgccgcg gcgacagcaa tcgaccttgg
     1261 tggcccgatc aacaaagccg caggttttgt tgccttcagc tttaccactg accacgtatt
     1321 accggtcacc gctcgttcta tcgctatcgt tattccgccg attggtctgg ggctggcgac
     1381 cattatcgac cgtcgtttaa ccggcaaacg cctgttcaac gctcaacttt atccgcaggg
     1441 taagaccgcc atgttcctgg cgtttatggg gatcagtgag ggtgcgattc cgtttgcact
     1501 ggaaagcccc atcaccgcca ttccgtcgta tatggtcggc gcgattgtcg gctcaaccgc
     1561 cgctgtctgg ctgggcgcag tgcaatggtt cccggaatcc gctatctggg catggccgct
     1621 ggtcactaac ctcggcgtct acatggcggg gatcgcgctg ggggcagtca taactgcgct
     1681 gatggtggtg ttcctgcgtc tgatgatgtt ccgtaaaggc aaattgttaa tcgatagcct
     1741 gtaagaagga aaatccagat gacattactc gcttcgctgc gcgactggct taaggcgcaa
     1801 caactggatg cggtgcttct ctcctcacgg cagaacaaac agccgcatct ggggatctcc
     1861 accggatcag gctatgtgct gattagccgt gaaagtgcgc acattctggt ggactcgcgc
     1921 tattacgcgg atgtagaagc ccgcactcag ggctaccagt tacatttgct tgatgcgacg
     1981 cacacgcttg caaccatcgt caataaaatc attgccgatg agcatctgca aacgctcggt
     2041 tttgaaggcc agcaggtgag ttgggaaacc gcgcaccgct ggcagtctga actcaatgcg
     2101 aaactggtta gcgccacgcc ggatgtgctg cggcaaatca aaacgccaga ggaggtggag
     2161 aaaatccgcc ttgcctgtgg gattgccgat cgcggtgcag agcatattcg ccgctttatt
     2221 caggcgggga tgagcgagcg cgagatagcc gctgaactgg agtggtttat gcgccagcag
     2281 ggcgcagaaa aagcctcttt tgacaccatt gttgccagtg gctggcgtgg ggcgctgccg
     2341 cacggcaaag ccagcgacaa gattgttgca gcgggcgagt ttgtcactct cgatttcggt
     2401 gcgctctatc agggctactg ctctgatatg acgcgcacct tgctggtgaa tggcgaaggg
     2461 gtgagcgccg aatctcatcc gctgtttaac gtctatcaga ttgttttgca ggcacagctc
     2521 gcagcaatct ctgcaattcg ccccggcgtg cgctgccagc aggttgacga cgccgcgcgt
     2581 cgggtgatta ccgaggcagg atttggcgac tattttggtc acaacaccgg tcatgctatt
     2641 ggcattgaag tacacgaaga tccgcgcttt tcaccgcggg ataccacgac gctacagcca
     2701 ggcatgttac tgaccgtgga gccggggatt tatttgccag ggcaaggggg cgtgcgcatc
     2761 gaggatgttg tgctggttac cccgcaaggc gcagaagtgc tctacgccat gccgaaaaca
     2821 gtgttgctca cgggagaggc ataatggatt tatcgctatt gaaagcgttg agcgaggcag
     2881 atgcgatcgc ctcctcggaa caggaagtgc ggcagatcct gctggaagaa gcggatcgcc
     2941 tgcaaaaaga agtgcgattt gatgggctgg gatcggtgct gatccgcctc aatgaatcga
     3001 caggtccgaa agtgatgatc tgtgcgcata tggacgaagt tggatttatg gtgcgcagta
     3061 tctcccgcga aggcgcaatt gatgtgctgc cggttggcaa cgtgcgcatg gctgcccgcc
     3121 agctgcagcc ggtgcgcatc accacccgtg aagagtgcaa aattccaggc ctgcttgacg
     3181 gcgaccggca ggggaatgac gtcagcgcca tgcgcgtgga tattggcgcg cgctcgtatg
     3241 acgaagtgat gcaggcggga attcgtccag gcgatcgcgt cacgttcgat accacttttc
     3301 aggttctccc ccaccagcga gtgatgggga aagcctttga tgaccgcctc ggttgctacc
     3361 tgctggtgac gttactgcgc gaactacaca gcgctgaact gcctgcggaa gtgtggctgg
     3421 tggccagttc cagcgaagag gtggggttac gcggcgggca aactgccacc cgcgcggtgt
     3481 cgccggacgt cgccattgtc cttgataccg cctgctgggc gaaaaacttt gattatggcg
     3541 cggctaacca tcgccagatt ggtaacggcc cgatgctggt gttaagcgac aagtcactga
     3601 ttgcgccgcc aaaactcacc gcctggatcg aaaccgtggc ggcagaaatt ggcgtgccgt
     3661 tacaggcgga tatgttcagt aacggcggca cggacggtgg agcggtgcac ttaaccggta
     3721 ctggcgtacc cacagtggtg atggggcctg ccacccgcca cggacattgc gccgcgtcga
     3781 ttgccgattg ccgcgatatt ttgcagatgg agcaactttt atctgccctt attcaacgtc
     3841 ttacgcgtga gacggttgtt caactgacgg atttcagatg atctcctgat taacccggag
     3901 cggttatgtt aacgattcaa tttctctgtc ctttgcctaa cggtctacat gctcgtccgg
     3961 cgtgggaact taaagaacag tgcagccagt ggcaaagcga aatcactttt attaaccatc
     4021 gccagaacgc aaaggccgat gccaaaagct cgctggcgct gattggcacc ggcaccctat
     4081 ttaatgacag ttgcagcctg aatattagcg gcagcgatga agagcaggcg cggcgcgtgc
     4141 tggaagagta catccaggtg cgctttatcg acagcgacag cgttcagcct acgcaggcag
     4201 aactgacggc gcatccgctg ccgcgttcat taagccgcct taacccggat ttactgtacg
     4261 gcaatgtgct ggcaagcggc gtcggcgtgg gtacgctgac cctgttacaa agcgacagcc
     4321 tcgacagtta tcgggcaatc cccgccagtg cgcaagattc cacccggctg gagcacagcc
     4381 tggcaacgct tgccgagcaa ctgaaccagc aattgcgtga gcgtgacggc gaaagcaaaa
     4441 ctatcctcag cgcccatttg tcgctgattc aggatgatga atttgcaggc aatatccgtc
     4501 gcctgatgac agaacagcat caggggctgg gggcggcgat catcagcaat atggagcagg
     4561 tttgcgccaa actttctgct tctgccagcg attatctgcg cgaacgtgtt agcgacattc
     4621 gcgatattag cgaacagttg ctgcatatta cctggccgga actgaagccg cgcaacaatc
     4681 tggtgctgga aaaaccgact attctggtgg ccgaggattt aaccccaagc cagtttttaa
     4741 gcctggattt aaaaaatctt gcgggcatga ttctggagaa aaccgggcgc acctcgcata
     4801 ccctgattct ggcccgcgcc tcggcgatcc cggtactgag cggcctgccg ctggatgcga
     4861 ttgcccgtta tgccgggcaa cctgcagtgc ttgacgccca gtgcggcgtg ctggcgatta
     4921 acccgaatga cgcggtgagc ggttattatc aggtcgcgca gacgctggcg gataaacgcc
     4981 aaaaacaaca ggcgcaggct gccgcgcagc tggcttattc ccgtgataac aagcgtattg
     5041 atatcgcggc gaatattggc accgctctgg aagcgcctgg cgcgtttgcc aacggcgcgg
     5101 aaggcgtcgg gctgttccgt accgaaatgc tgtatatgga tcgcgacagc gcgccggacg
     5161 agcaggagca atttgaagcc taccagcaag tgctactggc agcgggcgac aagccgatta
     5221 tcttccgcac aatggacatc ggaggcgata aaagcattcc ttatctgaat attccccagg
     5281 aagagaaccc gttcctcggc tatcgcgcgg tacgtattta cccggaattt gctggcctgt
     5341 tccgcactca actgcgggcc attttgcgcg ccgccagttt cggcaacgcc cagttgatga
     5401 tcccgatggt ccacagcctc gatcagatct tatgggtgaa gggcgagatc caaaaagcga
     5461 tcgttgagct taagcgcgat ggcctgcgtc atgcagagac gattacgctg ggtatcatgg
     5521 tggaagtgcc gtcggtgtgc tacatcatcg atcacttctg cgatgaggtc gatttcttca
     5581 gtatcggctc caacgatatg acccagtatc tgtatgcggt cgatcgtaat aacccgcgcg
     5641 tatcgccgct atataacccg attacgccat cgttcctgcg catgttgcag caaatagtta
     5701 ccactgcgca tcagcggggc aaatgggtag gcatttgcgg tgaactgggc ggtgaaagcc
     5761 gttatctgcc gctactgctt gggctgggcc tggatgagct gagtatgagt agcccgcgca
     5821 ttccggcggt gaaaagccag cttcgtcaac tggatagcga ggcgtgtcgg gaactggcac
     5881 gtcaggcgtg tgaatgtcgc agcgcgcagg aaattgaagc gttactcacc gcctttacgc
     5941 cggaagaaga cgttcgcccg ctgctggcgc tggagaatat cttcgttgat caggccttta
     6001 gcaataaaga gcaggcgatc cagttcctgt gcggtaacct cggcgttaac gggcgcactg
     6061 aacatccgtt cgagctggaa gaggatgtct ggcagcggga agagattgtc accactggcg
     6121 ttggttttgg cgtggcgatc ccgcacacca aatctcagtg gatccgtcat tccagtatca
     6181 gcattgcccg gctggcgaaa ccggttgact ggcagtcaga aatgggcgaa gtcgaactgg
     6241 tgatcatgct gacgctgggt gctaacgaag ggatgaatca tgtgaaagtc ttctcgcagc
     6301 tggcgcgtaa actggtgaat aaaaacttcc gccagtcgtt gtttgccgcg caagatgcac
     6361 aaagtatcct gacgctgctg gaaacagaat taaccttctg acgttagccc tgaaaacggg
     6421 cgctgtactc tcccggcgtc agaccaaact gacgccggaa aacgcgacaa aaatagtcgc
     6481 tatccggaaa accgcaacgc tgtgccactt catgaattga cagatgatat ttctgcaaaa
     6541 tcatcctcgc cttcgccatt cgcacccaac gcacatactc gataaaacgc atcgttccat
     6601 gctgagcaaa cagttttgac agatgattgg gcgtgatatt aaaaaactgc gccacgctct
     6661 cgcgggtgag cggctgggca taattatcct gaacccagtt acaaatgctg tgatagagaa
     6721 aatcgccgcg cggctgcgaa ttgcccggcg gcatattcac cacgttacgg caaagatgca
     6781 gcaaacttaa caccagcggc tgaatgatat tttgctccag cggcgaacgg ctaagatggg
     6841 tgagggcggt aagcatcgct tcaccttcac cgcgttgcag gctgggaagc tggatctggc
     6901 gagcaggatg caacaacgac gtggtgcggc tatcgtaaaa cgataaccca agccaggtcg
     6961 gagcgaacac caggcttaac agcatcaccg gtttgttgtt gatcggtaaa ttagccgcgc
     7021 gcgccggaat aaacagcatt tctccttcca ccaaacgttg ctgctttgct tccagcagat
     7081 tgccgtattc gccgcgcagc acgatatcca gacggggaaa atcaatacat aaactgccca
     7141 ccggcaacga ggcaggctgg ctggcaaacc agacgcgccc cagtagttgc gggttaagca
     7201 ccaggccgct gaacagatcg gcgaaaaatt gttgatcggc gggcaagcct ggtgctttca
     7261 tgaaagttcc cgattaaaga tgcattaact ggcgaaattc ttttactttg ctgcggctga
     7321 ctggcacttc aaaatccaga tctttcagcc gcagaatata ggtgttatta aaccacggtt
     7381 cgatttcgcg gattttgttc agattgacac aaaatgagcg atggcagcgg aaaaaatgcg
     7441 acggcggcag tttgctgcaa aattcggtaa tgttcatcgg cattacgtag gattcacgcc
     7501 gcgtatagac aaaggtcatt ttctcgtgcg cttcggcgta atagatatcg ttaattggcg
     7561 tgacgattat tcgctcatct ttcaccagat taatggtgtc attctcacgc gttaccgtcg
     7621 cggcaggcgt gctgctggtc tgctgttgtt gccaggccgc ttccagtttt tgcagcatcc
     7681 cggtaatgcg tgactcctga tacggtttta gaatgtagtc aaacgcctcc agttcaaacg
     7741 cttctaccgc atgttctttc cacgcggtga tgaacacaat aaacggttta tgggcgaact
     7801 ggctgatgtt ttgcgccagc aacacaccat ccagcgacgg aatattgata tccagaaaaa
     7861 tggcgtcgac gcggttatgc tgcaaaaact tcaacacgtc cagaccgtcg tcaaaggtgc
     7921 cgacaatctc catctggctg tgctctttaa ttagccagct cagttcctgt tgtgccagga
     7981 attcgtcttc aacaatgatg actttcacaa tatcactccg gctcaaagca ataacgtagc
     8041 ctgtgaggcg actggggtgc gttggttagg aatgtaaaac gcaatttccg ttcctggttc
     8101 caggcggcgg atatgcagcc cctcgccata caataacttc acgcgatgat gaacattcag
     8161 caggccaatt ttattgcccg gcatttcatt cgcttcgacc cgctcaatca cctttggatc
     8221 gatgccgtgc ccggtatccc gcaccgcaat gcgtacccga ttgccgcact ctgcaacgct
     8281 gatggtgaca acgcctttac ctttgcaagg ctggataccg tggacaatgg cattctccac
     8341 caacggctgg atcagcaggc tgggaatgca gcaattcacc tcttcatcaa tatcatagat
     8401 aaccgtgagc ttgtcgccaa agcgggcctg ctcaatggca atatagtctt taatttgata
     8461 cagctctttt ttgatatcga tttgctcatc gtcttttaat tcaatgttat agcgcagata
     8521 acgcgacaga ttaaagatca gctggcgagc agtatccgga ttcagacgga ttgacgatga
     8581 aatagcgttc aaagcgttaa acaggaaatg gggattaatt ttgctttgca gggcgcgaag
     8641 ctctgcctta tttgccattt cacgtagctg ctcggcgcgt gaaacctcca gttgcgtgga
     8701 gatgatttgc gacagaccaa ccgccatttc ctgtaatgac gaggttatct gatgtgcgtg
     8761 gcagtagtaa attttcagcg ttccggtcac aacgcctttc tcccaaagcg ggatcaccag
     8821 catcgagtgg atttccggcg tacggtgggc ttcatcattg tttttaatga tgatttttcc
     8881 gtaattcatc gcctgacggg tcgtcgggct aatgaagtca tcgccattct gatagttatg
     8941 ctcacccacg ccaacatagg ccagcacatg atcggtattg gtaatcgcca ccgcatcggc
     9001 gtgaatgtca tcgcggataa tttcgcagac cttgcgtaat gactcgctat tgacatggcg
     9061 aaacagaggt agcgttttgt tggcgatatc cagcgccaac ttggcctgcc gcgcggcgct
     9121 ggcctctttt tcgccctcaa cgctttgcac cagaagcaca ataaagccga tacagacgct
     9181 gccgaggatc attggaatcc cgattttaga gacaatatcg atccccagcg cggtagttgg
     9241 tgcccagaca atcaccagaa tcatggtcaa cgtttcacac aacatgccgc cgagaatacc
     9301 gacgcgccag cgctgtgctt tggggatttt caggttgatc cagcccgata tacaacccgc
     9361 cagaatgctg gtgataaagc aggggatcgc cgtcacgccg ccgatatcaa ttaaataccg
     9421 gtgaataccc gcaatcacgc cggtaatgat gcctacccac gggccaaaca gaatcccgcc
     9481 ggacatcacc gcgataatac gtacgttcac cagcgagcct tctacgggaa cgccggacca
     9541 ggtgctgaac agggcgaaca gcgagaaaat ggcggtaacg gcgagcaatt cttttgggga
     9601 gtgcgccgac ttgtgcaaca gttcgcgaaa caggcggata cggatgagaa agaacaggca
     9661 gataagcatt aacgctgccc gatcgaagac cgccagcagc atgttgaata tttcgtgcac
     9721 gggtgactct gaataaggat taaacctcta tgataggtaa cctgaaggct gatgaccagc
     9781 aggccgtttt tgaggaaaaa ccataatttt aaaaggggat tgatagataa atggtgtatt
     9841 ag
//
LOCUS       DAPGOF010000184         9814 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.185, whole genome shotgun sequence.
ACCESSION   DAPGOF010000184 DAPGOF010000000
VERSION     DAPGOF010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.185"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            165..380
                     /gene="bdm"
                     /locus_tag="RHV13_003983"
     CDS             165..380
                     /gene="bdm"
                     /locus_tag="RHV13_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HDV1243458.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            482..619
                     /gene="sra"
                     /locus_tag="RHV13_003984"
     CDS             482..619
                     /gene="sra"
                     /locus_tag="RHV13_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HDV1243459.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            776..2473
                     /gene="maeA"
                     /locus_tag="RHV13_003985"
     CDS             776..2473
                     /gene="maeA"
                     /locus_tag="RHV13_003985"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HDV1243460.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFIDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            2607..3617
                     /gene="adhP"
                     /locus_tag="RHV13_003986"
     CDS             2607..3617
                     /gene="adhP"
                     /locus_tag="RHV13_003986"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HDV1243461.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3763..4047
                     /locus_tag="RHV13_003987"
     CDS             3763..4047
                     /locus_tag="RHV13_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HDV1243462.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQIWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(4098..4751)
                     /gene="fdnI"
                     /locus_tag="RHV13_003988"
     CDS             complement(4098..4751)
                     /gene="fdnI"
                     /locus_tag="RHV13_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HDV1243463.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4744..5628)
                     /gene="fdnH"
                     /locus_tag="RHV13_003989"
     CDS             complement(4744..5628)
                     /gene="fdnH"
                     /locus_tag="RHV13_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HDV1243464.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5641..8688)
                     /gene="fdnG"
                     /locus_tag="RHV13_003990"
     CDS             complement(5641..8688)
                     /gene="fdnG"
                     /locus_tag="RHV13_003990"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8101..8103),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HDV1243465.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKEHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNIGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLAGQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8920..9801
                     /gene="yddG"
                     /locus_tag="RHV13_003991"
     CDS             8920..9801
                     /gene="yddG"
                     /locus_tag="RHV13_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HDV1243466.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSTPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         2385 a         2585 c         2477 g         2367 t
ORIGIN      
        1 caccacaata ataataccta tcaatatcat agtgttaaaa tataaaactc atcaaccagg
       61 actaatctta acaacgaagc ggcaaatatt tgccatgctg aatgtgcttt ataaatctgc
      121 gatccgtagc agacatcata aatacacaga cacggagaat cactatgttt acttattatc
      181 aggcagaaaa ttcaacagca gaacccgctc tggttaatgc cattgagcaa ggccttcggg
      241 cagagcacgg tgttgtcact gaagatgaca ttctcatgga gctgaccaaa tgggtggaag
      301 cttctgataa cgacatcctc agtgatatct accagcaaac gatcaattat gtggtcagtg
      361 gccagcaccc tacgctttaa ggtgctatgc ttgatcggca acctaattta ggggtttagc
      421 acgtgtttct tcgctacggc gatgttgtcc ttaaaactag caacaggatt gaggagttaa
      481 aatgaaatcg aaccgtcagg cacgtcatat tcttggactg gaccataaaa tatctaacca
      541 gcgcaaaata gttaccgaag gtgacaaatc cagcgtggta aataacccaa ccggcagaaa
      601 acgccccgct gaaaagtaat tcataaccat cagtcctcaa tgacgattaa acaccattgc
      661 ctgcgcaatg gtgtttttgt ttttatctgc tttatacttg gggccgacgc cctggcggta
      721 aagcaaagac gataaaagcc ccccagggat ggatattcaa aaaagagtga gtgacatgga
      781 accaaaaaca aaaaaacagc gttcgcttta tatcccttac gctggccctg tactgctgga
      841 atttccgttg ttgaataaag gcagtgcctt cagcatggaa gaacgccgta acttcaacct
      901 gctggggtta ctgccggaag tggtcgaaac catcgaagaa caagcggaac gagcatggat
      961 ccagtatcag ggattcaaaa ccgaaatcga caaacacatc tacctgcgta acatccagga
     1021 caccaacgaa accctcttct accgtctggt aaacaatcat cttgatgaga tgatgcctgt
     1081 tatttatacc ccaaccgtcg gcgcagcctg tgagcgtttt tctgagatct accgccgttc
     1141 acgcggcgtg tttatctctt accagaaccg ccacaatatg gacgatattc tgcaaaacgt
     1201 accaaaccat aatatcaaag tgattgtggt gactgacggt gaacgtattc tggggcttgg
     1261 tgaccagggc atcggcggga tgggcattcc gatcggtaaa ctgtcgctct acaccgcctg
     1321 tggcggcatc agcccggcgt atacccttcc ggtggtgctg gatgtcggca ccaacaacca
     1381 acagctgctt aacgatccgc tgtatatggg ctggcgtaat ccgcgtatca ctgacgacga
     1441 atactatgaa ttcattgatg aatttatcca ggctgtgaaa caacgctggc cggacgtgct
     1501 gttgcagttt gaagacttcg cacaaaaaaa tgcgatgccg ttacttaacc gctatcgcaa
     1561 tgaaatttgt tcctttaacg atgacattca gggcactgcg gcggtaacag tcggcacact
     1621 gatcgcagca agccgcgcgg caggtggtca gttaagcgag aaaaaaatcg tcttccttgg
     1681 cgcaggttca gcgggatgcg gcattgccga aatgatcatc gcccagaccc agcgtgaagg
     1741 attaagcgag gaagcagcac ggcagaaagt ctttatggtc gatcgctttg gcttgctgac
     1801 cgacaagatg ccgaacctgc tgcctttcca gaccaaactg gtgcagaagc gcgaaaacct
     1861 cagtgactgg gataccgaca gcgatgtgtt gtcactgctg gatgtggtgc gcaatgtaaa
     1921 accagatatt ctgattggcg tctcaggaca gaccgggctg tttacggaag agatcatccg
     1981 tgagatgcat aaacactgtc cgcgtccgat cgtgatgccg ctgtctaacc cgacgtcacg
     2041 cgtggaagcc acaccgcagg acattatcgc ctggactgaa ggtaacgcgc tggtcgccac
     2101 gggcagtccg tttaatccag tggtatggaa agataaaatc taccctatcg cccagtgtaa
     2161 caacgccttt attttcccgg gcatcgggct gggtgttatt gcttccggcg cgtcacgtat
     2221 caccgatgag atgctgatgt cggcaagtga aacgcttgct cagtattcgc cgctggtcct
     2281 gaacggcgaa ggtctggtac tgccggaact gaaagatatt cagaaagtct cccgcgcaat
     2341 tgcgtttgcg gttggcaaaa tggcgcagca gcaaggcgtg gcggtgaaaa cctctgccga
     2401 agccctgcaa caggccattg acgataattt ctggcaagcc gaataccgcg actaccgccg
     2461 tacctccatc taagcctgcg cccggtagtg aaggctaccg ggctatttcc ctctcccttt
     2521 ttcagatctc atccatactg ggtagtggcg aataaatctc atttgcctca cctgctatgc
     2581 agaacatcat ccgaaaagga ggaactatga aggctgcagt tgttacgaag gatcatcatg
     2641 ttgacgttac ggataaaaca ctgcgctcac tgaaacatgg cgaagccctg ctgaaaatgg
     2701 agtgttgtgg tgtatgtcat accgatcttc atgttaagaa tggcgatttt ggtgacaaaa
     2761 ccggcgtaat tctgggccat gaaggcatcg gtgtggtggc agaagttggc ccaggtgtca
     2821 cctcattaaa accaggcgat cgtgccagcg tggcgtggtt ttacgaagga tgcggtcatt
     2881 gcgaatactg taacagtggt aacgaaacgc tctgccgttc agttaaaaat gccggataca
     2941 gcgttgatgg cgggatggcg gaagagtgca tcgtggtcgc cgattacgcg gtaaaagtgc
     3001 cagatggtct ggactcggcg gcggccagca gcattacctg tgcgggggtc accacctaca
     3061 aagccgttaa gctgtcaaaa attcgtcccg ggcagtggat tgctatctac ggtcttggcg
     3121 gtctgggtaa cctcgccctg caatacgcga agaatgtctt taacgcgaaa gtgatcgcca
     3181 ttgatgtcaa tgatgagcag ttaaaactgg caaccgaaat gggtgcagat ttagcgatta
     3241 actcacgcac cgaagacgcc gccaaaattg tgcaggagaa aaccggtggc gctcacgctg
     3301 cggtggtaac agcggtagct aaagctgcgt ttaactcggc agttgatgct gtccgtgcag
     3361 gtgggcgtgt tgtggcagtc ggtctgccgc cggagtctat gagcctggat atcccacgcc
     3421 ttgtgctgga tggcattgaa gtggtcggtt cgctggtcgg cacgcgccag gatctaaccg
     3481 aagccttcca gtttgccgcc gaaggtaaag tggtgccgaa agtcgccctg cgtccgttag
     3541 cggacatcaa caccatcttt accgagatgg aagaaggcaa aatccgtggc cgcatggtga
     3601 ttgatttccg tcactaagag gcctttgctg cgactcccat gttcgggtcg cagcatcgca
     3661 cactctccaa catgaaatgg ctaaatggat tattgttgcg taggaaggaa caataatgat
     3721 agtgttccca gagaaggaac gctactgaga gtaaagacac taatgaaaat ggccaatcat
     3781 ccccgcccgg gggacattat tcaggaatca ctggacgaac ttaatgtcag cctgcgcgag
     3841 tttgccagag caatggaaat tgctccctca acggcaagcc gattgctgac aggaaaagca
     3901 gctttgacgc cagaaatggc aattaaactt tccgtggtga tcggcagttc gccgcaaatt
     3961 tggctgaatc tgcaaaacgc ctggagtctg gcagaggctg aaaaaacggt ggatgtgtcg
     4021 cgattacgcc gtctggttac gcaataaaaa aatggcgctt cttaaagcgc cattttcagc
     4081 ttaaagttca aaagagatta tatcccttct tcactctctt ttttcgcttc tgccttctcg
     4141 atttcacgat accagcgcgg atggtgtttc ttcgcccagc gacggctcac cttcccttcg
     4201 atcatccctt taatcgaccc tttcacccag aatgccatat acatatggat caggatggcg
     4261 tggatcagga tgatacccgc agccgcgtgg atcagcaggc tgtagcgaac aacctgcatc
     4321 gggaagtact gcgcaaagta cggacgccag ataatcaccc cggtcaccag cagcacgaaa
     4381 atcatgctca tgatcgacca gaacatcatc ttttgcccgg cgttgtactt accaacatcc
     4441 gccactttgt gctcattgcc ttttaatact tcgacaatgt tcaacagcca cggaatatct
     4501 ttcttatccg ggatgttgtg atgcacaaaa cgcacaaaca taaacatcag tgcgacgaaa
     4561 atcgcaatgc cgaagaacgg gtgcaaaatg cgtcccatct gcggcgtacc gaaggtttgc
     4621 gtcagccatt gcagcgtcgg gaagaagaac gaaatcccgg acagcgccac caggaagaag
     4681 caaatcacca cggtccagtg acaggcgcga tcaataaatt tggtgcgcac aatcattttc
     4741 gacttactca tgatgatcct cctcgtcatc gtccacttcc ttattcgggc caataccgat
     4801 gtagtggaaa atcaacccgg caaaagtggc aataaagcca gccgctgcca gcggtttcaa
     4861 cgcgcctttc cacaggctta ccgaggtgtc gatcttcgga tctttcggca gaccgtgata
     4921 cagctccggc tgatcggcgt gatgcaacac gtacataacg tgcgtaccac cgaccccttc
     4981 cgggttgtag acgccagcat gttcgtaacc acgcgctttc agtttcgcca cgcgctgttc
     5041 cgccagctcc agcatctcct tcttggtgcc gaagtggata gccccggtcg gacaggtttt
     5101 cacacaagcc ggttcctggc cgacgctgac gcgatcgacg cagagcgtgc atttatatac
     5161 ccggttatcc tctttgttga ggcgcggaat attaaacgga cacccggcaa tgcagtaacc
     5221 acagccgatg cagttttccg actggaaatc gacaatcccg ttagcgtact gaatgattgc
     5281 accagcagac gggcacgcct tcaggcagcc cggatcttca cagtgcatac agccgtcttt
     5341 acggatcagc cactccagct tgccgttctg ttcggtttcg ctaaagcgca tcaccgtcca
     5401 ggacttggcg ctcagatcgg cggggttatc gtaaaccccg acgcagtgcc ccacttcatc
     5461 acggatgtcg ttccactccg aacacgccac ctgacaggct ttacagccga tacaggtgga
     5521 aacgtcgata agtttcgcga cttctgcttt gtaatcacgc acctgagaag gcggcgtgat
     5581 ggagttagtt gcggaccttt tgataatgtc ctgcgtttcc atagccatct gttcgccccc
     5641 ttacgccttc tcgatgttga ctaagaacgc tttatattcc ggcgtttgcg agtttgcatc
     5701 accgacattc ggcgtcagag tgttagcgat ataaccttta cgcgcgacac cctcaaagcc
     5761 ccagtggatt ggaataccca ccgtttcaac ctgctgaccg tttacattca gcggcttcag
     5821 acggcgcgtt accacagcca ccgcgcggat aaatccacgc ttgctggaga cagtgacacg
     5881 atcgccatta ttaatgcctt tcgccgccgc cagcgtttcg ctgatttcca caaactgttc
     5941 cggctgagca attgcgttga gcaatgcgtg cttggtccag gtgtggaagt gctcggtcag
     6001 acgataggtc gtacccacat acgggaactg ctctttttta cccatccgca gcgcgtcttg
     6061 ttcatacaga cgaacaaccg ggttagacac cacgttcgga tgcagcgggt tagtgcccag
     6121 cggcgtttca atcggctcgt agtgttccgg gaacggacct tccgccattt tgttgatggc
     6181 aaacaggcgt cccatccctt ccggctgcat gataaacggc ccggttggcg tacccggtgc
     6241 ggcattgccg aagtcaggaa tatcgttacc cgtccacttg ctgccgttcc actggatcag
     6301 catccgtttc ggatcccacg gtttaccgtt gatatccgcc gaagcacggt tgtacagcac
     6361 gcgacggttg agcggccacg cccaggccca tcccagcgta ttccccagac cggacgggtc
     6421 ggagttatcg cggttagcca tctggttgcc ctgctctgtc cagctaccgg tgtagatcca
     6481 gcaagatgat gcggttgtac cgtcatcacg cagatgcgca aagctactca gcaactgacc
     6541 tttcttcgca atcagcacgc catttgcgtc atagagatct tccagcgcat agccgttgtt
     6601 ctctttagct acttcgtcag attgcggttc gtgcggctgc ttgtagttcc agctcatctt
     6661 catcagcggt tctacgcctt taccaccttc ggcctggtac aactcgcgca gatgatggta
     6721 gatacccgcc agaatttcgc cgtcgttacg cgcttcgccc ggcgcgtcct gacctttcca
     6781 gtgccactgc aaccagcgac cggagttagc gatagaacca tcctcttcag caaagcaggt
     6841 cgaaggcaga cggaatactt cagtctgaat agacgccgga tcgacatcgt tcgactcgcc
     6901 gtggttctgc cagaaggtag aggtttcagt caccagcgga tcgataacca ccatgtactt
     6961 cagcttgctc aggcagctca ccactttgtt tttgtccggg aaggacgcaa ccgggttaaa
     7021 gccctggcag aaataaccgg taaccttgcc ttcgtccatc atgttgaaat acttgatgac
     7081 gtcgtaagtc tggtcccact tcggcagcca gtcatagccc cagttgttct ctttctgcgc
     7141 ggcatcgcca tagaaagatt tcatcaggct aacgaagaac ttcggatagt tgctccagta
     7201 gttcacctga ccagccagtg tcgctttcgg cgtgttcgct tccagatacg actgcaaatc
     7261 aacctgtttt tctgacggca gcgtcagata accaggcagg ctggtagaga gcaggcctaa
     7321 gtcagtcaga ccctgaatgt tggagtgacc acgcaatgcg ttcacgccgc caccggccat
     7381 acctatgtta ccgagcagca actggatcat cgccatagta cggatgttct gcgcacccac
     7441 ggtgtgctgc gtccagccca gcgcgtacag gaaggtggtt gtgcgatccg gtgcgctggt
     7501 ggaggccagc acttcacaca ctttcaggaa gtcggctttt ggcgtaccgc agatgttttc
     7561 aacgacgtcc ggcgtgtagc gggaaacgtg ctctttcagc aggttccaca cacagcgcgg
     7621 atgagtcagt gtttcatcgc gtttcgcata gccgttttca tcgaactgat agttccagga
     7681 cgatttatcg tactggcgtt tttcagcgtc ataaccgctg aacaggccgt cttcgaaagc
     7741 aaaatcatca cgcaccagca agctggcgtt agtgtaatgc ttaacatatt cggcgttgat
     7801 tttgttgttt tcgatcaggt agcgcaaaac gccagacagg aacgtaatgt ccgtaccgga
     7861 acgaataggt gcgtaaatat ccgctacaga agcggtacgc gtaaaacggg gatcgacaac
     7921 gatcaaggtt gcatcgttgt tgtttttcgc ttccatcgcc cagcggaaac cgacgggatg
     7981 cgcttcagca gcgttaccgc ccattaccat tacgacgtta gcgtttttga tatccaccca
     8041 gtggttggtc atcgcaccgc gaccaaatgt tggagcaaga cttgctaccg ttggtccgtg
     8101 tcagacgcgt gcctggttgt ctaccgccag catcccgagg gagcgggcaa atttctgcgt
     8161 cagcatcccg gtttcgttgc tggcaccgga agcacacagc ataccggtag aaagccaacg
     8221 gtttaccgtt acgccctgct cgttcttttc aataaagtta gcgtcacggt cagctttcat
     8281 cagcttcgca atacgggaga atgcttcttc ccagctaatg cgctgccatt tgtcagaacc
     8341 tggcgcacga tattccgggt agcgcagacg gttttcgctg ttgacgtaat ccagcaaacc
     8401 ggcccctttc ggacacagcg caccacggct taccggatga tccgggtcac cttcaatgtg
     8461 ataaatcgct tctctggcgt ttttagcgcc atcacccagg ctatacatca atagcccgca
     8521 acctacggaa cagtatgtgc aggtgttacg gatctcttta gcgcgtaata atttgtagtt
     8581 tcgcgcctga gccagtgctt gcttcggggc aaagcccaat gccgctactg ttgttccagc
     8641 cataccgccc gcgcagattt taaaaaattg tctgcgactg acgtccattg ctttcctctt
     8701 ttttcagggg tattactgcg cgaggaaact aacagtaaaa ttctgaatct ttttgcgcga
     8761 aatcaaaaag aacatctgtt tgccacaaaa taatccgcct gcgcaaattg acctacctca
     8821 atagcggtag aaaaacgcac cactgcctga caggccagtt aaaaaaatgc tataaaattc
     8881 agcttaattt ttaacggcaa aagagacaaa acagcgagca tgacacgaca aaaagcaacg
     8941 ctcatagggc tgatagcgat cgtcctgtgg agcacgatgg taggattgat tcgcggtgtc
     9001 agtgaggggc tcggcccggt cggcggcgca gctgctatct attcattaag cgggctgctg
     9061 ttaatcttca cggttggatt tccgcgtatt cggcaaatcc cgaaaggcta tttactcgcc
     9121 gggagtctgt tattcgtcag ctatgaaatc tgtctggcgc tttccttagg gtatgcggcg
     9181 acccgtcatc aggcgattga agtgggtatg gtgaactatc tgtggcccag cctgacaatt
     9241 ctctttgcca ttctgtttaa tggtcagaaa accaactggc tgattgtacc aggattatta
     9301 ttagccctcg tcggcgtctg ctgggtgtta ggcggtgaca atgggttaca ttatgatgaa
     9361 atcatcaata atatcaccac cagcccattg agttatttcc tggcgttcat tggtgcgttt
     9421 atctgggcag cctactgcac agtaacgaat aaatacgcac gcggatttaa cggaattacc
     9481 gtttttgtcc tgctaacggg agcaagtctg tggatttact attttctcac gccacaacca
     9541 gaaatggtat ttagcacgcc cgtcatgatt aaactcatct ctgctgcatt taccttagga
     9601 tttgcttatg ctgcatggaa tgtcggtata ttgcatggta acgtcaccat aatggcggta
     9661 ggttcgtatt ttacgcccgt actttcctca gcgcttgcag ccgtgctgct cagcaccccg
     9721 ctgtcgttct cgttctggca aggcgcgcta atggtctgcg gcggttctct gctctgctgg
     9781 ctggcgacac gtcgtggtta aaataattgc cggg
//
LOCUS       DAPGOF010000185         9524 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.186, whole genome shotgun sequence.
ACCESSION   DAPGOF010000185 DAPGOF010000000
VERSION     DAPGOF010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.186"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            31..645
                     /gene="evgA"
                     /locus_tag="RHV13_003992"
     CDS             31..645
                     /gene="evgA"
                     /locus_tag="RHV13_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HDV1243467.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            650..4243
                     /gene="evgS"
                     /locus_tag="RHV13_003993"
     CDS             650..4243
                     /gene="evgS"
                     /locus_tag="RHV13_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HDV1243468.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEIIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     ESPFKDVFSNTHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPEHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEITQQVAAVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLNCGMNLCLFKPLTLDVLKTHLSQLHQVAHIVPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLSAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQQNN"
     gene            complement(4299..5444)
                     /gene="yfdE"
                     /locus_tag="RHV13_003994"
     CDS             complement(4299..5444)
                     /gene="yfdE"
                     /locus_tag="RHV13_003994"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HDV1243469.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(5518..6462)
                     /gene="yfdV"
                     /locus_tag="RHV13_003995"
     CDS             complement(5518..6462)
                     /gene="yfdV"
                     /locus_tag="RHV13_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HDV1243470.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(6532..8226)
                     /gene="oxc"
                     /locus_tag="RHV13_003996"
     CDS             complement(6532..8226)
                     /gene="oxc"
                     /locus_tag="RHV13_003996"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HDV1243471.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVASAISLLAKAERPLVIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(8280..>9524)
                     /gene="frc"
                     /locus_tag="RHV13_003997"
     CDS             complement(8280..>9524)
                     /gene="frc"
                     /locus_tag="RHV13_003997"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HDV1243472.1"
                     /translation="TPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDVTR
                     HQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAIDH
                     MGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPPLV
                     SAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRLDK
                     LGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQNWE
                     NTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIPCA
                     PVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEHTA
                     AVLQELGYSDDEIAAMKQNHAI"
BASE COUNT         2813 a         2182 c         1971 g         2558 t
ORIGIN      
        1 ggagatgctt cattgcaaag ggaataatct atgaacgcaa taattattga tgaccatcct
       61 cttgctatcg cagcaattcg taatttattg atcaaaaacg atattgaaat cttagcagag
      121 ttgactgaag gcggaagtgc cgttcagcgg gtggaaacac ttaagcctga tatcgtcatc
      181 attgatgtcg atatccccgg agttaacggt atccaggtgt tagaaacgct gagaaagcgt
      241 caatatagcg gaattattat tatcgtttcc gctaaaaatg accattttta cgggaaacat
      301 tgtgctgatg ctggcgctaa tggtttcgtg agtaaaaaag aaggcatgaa caatatcatt
      361 gcggctattg aagctgcaaa aaatggctac tgctatttcc ccttctctct caaccggttt
      421 gttggaagtt taacgtccga ccagcaaaaa ctcgactcct tatcgaaaca agaaattagt
      481 gtcatgcgct atattcttga tggcaaggat aataatgaca ttgctgaaaa aatgttcatc
      541 agcaacaaaa ctgtcagcac ttataaaagt cgcctgatgg aaaaattaga atgtaaatca
      601 ctgatggatc tttacacatt cgcacaacgt aacaaaatcg gctaaccaca tgaagttttt
      661 accctatatt tttcttctct gttgtggtct ttggtcgacc ataagtttcg cagacgaaga
      721 ttacatcgaa tatcgtggca tcagtagtaa caaccgtgtc acacttgatc cactacgtct
      781 gagcaacaag gaattacgtt ggttagcgag caaaaaaaat cttgtgattg cagtacataa
      841 gtcccaaacg gctacgttgt tgcataccga ttcgcagcaa cgggttcgtg gtattaatgc
      901 tgattattta aatcttttaa aaagagcgtt aaatatcaaa ttaacactcc gggaatacgc
      961 cgatcatcaa aaagcaatgg acgcgctgga agaaggtgaa gtcgatatag tgttatcaca
     1021 tttagttgct tcgccgcctc ttaatgatga cattgctgca accaaaccac tgataattac
     1081 ctttccggcg ctggtcacca cccttcacga ttcaatgcga ccgcttacct catcaaaacc
     1141 agtaaatatt gctcgagtag caaattatcc ccctgacgag ataattcatc aatcatttcc
     1201 aaaagcaaca attatctctt ttacaaattt atatcaggca ttagcatccg tctcagccgg
     1261 acagaatgat tactttattg gtagtaacat cattaccagc agtatgattt cccgctattt
     1321 cactcactcc ttaaatgtag tgaaatatta taactcaccg cgtcaatata acttcttctt
     1381 aaccagaaaa gaatctgtca ttcttaatga agtactcaat cgatttgttg atgctttaac
     1441 aaatgaagtt cgctatgaag tatcacaaaa ttggcttgat acaggaaacc tagcctttct
     1501 gaacaagcca ttagaactca ctgaacatga aaaacagtgg attaagcagc atcccgattt
     1561 aaaggtgctg gaaaatcctt actcgccccc ctattctatg acggatgaaa atggctcggt
     1621 tcggggcgtt atgggggaca ttcttaatat tattaccttg caaacaggtt taaatttttc
     1681 tccgatcacc gtttcacaca atatccatgc cggaacacag ctgaatcccg gcggatggga
     1741 tatattaccc ggtgctattt atagtgaaga tagagaaaat aatgttttat ttgctgaagc
     1801 cttcataaca acgccttacg tttttgtcat gcaaaaagcg cctgacagtg aacaaacatt
     1861 aaaaaaagga atgaaagttg ccattccata ttattatgag ctgcattcgc aattaaaaga
     1921 gatgtatccg gaggtagagt ggataaaagt cgataacgcc agcgctgcat ttcacaaggt
     1981 caaggaaggt gaacttgatg ctctggtcgc gacacagcta aattcgcgtt acatgatcga
     2041 ccattactat cctaatgaac tttatcattt tcttattccc ggcgttccga atgcatcgct
     2101 ttcgttcgct tttcctcgcg gagaaccgga acttaaggat attattaata aagcactgaa
     2161 tgcaatcccc ccaagcgaag ttctgcgcct gacggaaaaa tggattaaaa tgcccaatgt
     2221 gaccattgac acatgggacc tctatagcga gcaattttat attgttacga cattatccgt
     2281 tttattagtt ggcagtagcc ttttatgggg attctacctg ttacgctcag ttcgtcgtcg
     2341 taaagtcatt cagggtgatt tagaaaacca aatatcattc cggaaagcgc tctcggactc
     2401 cttaccgaat ccaacttatg ttgtaaactg gcaaggtaat gtcattagtc acaatagtgc
     2461 atttgaacat tatttcactg ctgattacta caaaaatgca atgttgccat tagaaaacag
     2521 tgaatcaccc tttaaagatg ttttttctaa tacgcatgaa gtcacagcag agacgaaaga
     2581 aaaccgaaca atatacacac aggtatttga aattgataat ggcatcgaga aaagatgcat
     2641 taatcactgg catacattat gcaatcttcc tgcaagtgac aatgcagtat atatttgtgg
     2701 ttggcaagat attactgaaa cgcgtgatct aattaatgca ctcgaggtag aaaaaaataa
     2761 agcgataaag gctaccgtag caaaaagtca gtttctggca acgatgagtc acgaaataag
     2821 aacaccaata agctctatta tgggcttcct ggaacttctg tcgggttctg gtcttagcaa
     2881 ggaacaacgg gtggaggcga tttcacttgc ctacgccacc ggacaatcac tcctcggctt
     2941 aattggtgaa atccttgatg tcgacaaaat tgaatcgggt aactatcaac ttcaaccaca
     3001 atgggtcgat attcctactt tagtccagaa cacttgtcac tctttcggtg cgattgctgc
     3061 aagcaaatcg atcgcattaa gttgcagcag tacgtttcct gaacattacc tggttaagat
     3121 cgaccctcag gcgtttaagc aggtcttatc aaatttactg agtaatgctc tcaaatttac
     3181 caccgagggg gcagtaaaaa ttacgacctc cctgggtcac attgatgaca accacgctgt
     3241 aatcaaaatg acgattatgg attctggaag tggattatca caggaagaac aacaacaact
     3301 gtttaaacgt tatagccaaa caagtgcagg tcgtcagcaa acaggttctg gtttaggctt
     3361 aatgatctgc aaagaattaa ttaaaaacat gcagggtgat ttgtcattag aaagtcatcc
     3421 aggcatagga acaacattta cgatcacaat cccggtagaa attacccaac aagtggcggc
     3481 tgtcgaggca aaagcagaac aacctatcac actacctgaa aagttgagca tattaatcgc
     3541 ggatgatcat ccgaccaaca ggctattact caaacgccag ctaaatctat taggatatga
     3601 tgttgacgaa gccactgatg gtgtgcaagc gctacacaaa gtcagtatgc aacattatga
     3661 tctgcttatt actgacgtta atatgccgaa tatggatggt tttgagttga ctcgcaaact
     3721 ccgtgagcaa aattcgtcct tacccatctg ggggcttaca gccaacgcac aggctaacga
     3781 acgtgaaaaa gggttaaatt gcggcatgaa cttatgtttg ttcaaaccgt tgaccctgga
     3841 tgtactgaaa acacatttaa gtcagttaca ccaggttgcg catattgtac ctcagtatcg
     3901 ccaccttgat atcgaggccc tgaaaaataa tacggcgaac gatctacaac tgatgcagga
     3961 gattctcatg actttccagc atgaaacaca taaagattta tccgctgcgt ttcatgcact
     4021 agaagctggc gataatagaa ctttccatca gtgtattcat cgcatccacg gtgcggctaa
     4081 catcctgaat ttgcaaaagt tgattaatat tagccatcag ttagaaataa cacctgtttc
     4141 agatgacagt aagcctgaaa ttcttcagtt gctgaactct gtaaaagaac acattgcaga
     4201 gctggaccag gagattgctg ttttctgtca acaaaataac taaatagtgg ctcccacaat
     4261 gttcaaatgt gggagctatt taccagcaca tcttacattt atgatgagaa ctcctggcga
     4321 atttgttccc catgctggtc gagcgttgcc gctcccggca taacatgcgg gtccgcgcag
     4381 ccgctgattt ttatcggatt acccggcatc attattcctc cggcttcaat caacatattt
     4441 ctcgcctgag tttgtggcaa attaatggcc tcagccacac ttaataacgg cgctacgggc
     4501 acaccgactt catgtatttt ggctaaccaa acttcagctg cctgcgtttt taacgtccgc
     4561 tcaatatatt gtttaagaat agcctggttt tgtacgcgta aaatattgct gctaaatctg
     4621 ggatcattaa ccagttccgt aagctccagt gcctggcata acgcagaaaa aagcttgtca
     4681 ttaccacaac aaatcgtaat cggcttatcc tgagtatcga aaacatcaaa aggtgccatg
     4741 tagggatggc gatttcccag gcgttgtggt gacttccctg tcgcaatata tgccatcaga
     4801 ccatgctcca gaaaactcag cgtggcatca aacatcgcta tatcgacatg cgcccctctt
     4861 tggctctttt cgcggccata aagtgcactc actattccgc tgaataaata aacaccgccg
     4921 catagatccg caagagaggt accaacacgc actggcggag catcagggta ccctgtttcc
     4981 atcattatcc cgctcattgc ctgaatgatg gtatcgtagg caggagcatc ttttagcgga
     5041 ccggtatgtc cgaaacctga cgatgaagca tatatgaggc gcgggttgat ttcttgtaac
     5101 ctttcccatg aaaaccctag tttttccatt gtacctgggc gaaaattctc agctaataca
     5161 tcagcttgtt taagcatatt tataaatata cttttatcgt gatcattctt taaatcaaga
     5221 accacactct ctttgccatg attaataaaa ctgtaataga gtgactgtcc atccacatag
     5281 ggaccaaatg tgcgggtatc atcaccatgg cccggcggct caactttaat tacccgtgcg
     5341 cccatattac aaagaagttg agttccgaaa ggtccattaa ggacatgtgt catatcgata
     5401 actaataagc cttcaaacgg ccctttgctt tcattatttg tcatttgctt atgccttgaa
     5461 aaaagccgaa ggtgaaaaga cacttcggca tttatgtaaa atcaataaca tgattaatta
     5521 tgaaaccagt cgactgacat aaatccacaa gggagccgtg acgacaaaac ccagtacgct
     5581 cacagccaat gacgctgtac cagtacgggt gtagacatta aaccggctgg caataatgat
     5641 tccggagaat gccggcggta atgcgcctgc cagtaccatc atctgcagat gttcgctgtt
     5701 caaatgacat gccataccaa cgagaagcag tgccagtggc atcagaatca gcttcaggaa
     5761 ggtgttataa gcaatttcgg cactgaactc gaatttatgt gcagccagag tcaaccccgc
     5821 agcgaatacc gctacccctg agttagcctt cgcaatcaga ttaaaggttg ggtcccatgc
     5881 tgccggaatt tttaccccaa ccaacaccag gatcgttgcc agaacaggtg cccatacaac
     5941 tggctccttt gccgcagaaa ttaatgcgct cagattacta ttcttcttac catccgctcc
     6001 tgaggaagga ttcagcaaat acagaccaat aggaatagta attgcgttaa caataataga
     6061 aataattgct accactaaac ctgttgatac ggaatcacca taaataggat cgagaactgc
     6121 aaaccccagg aatccaatgg taggtgaacc tgcaattaat gcacatacag ctgcttctgc
     6181 atgagtacgt ttaaaaaatt tgtagcaacc gaaccaggag aaaaagaaac atccgacaat
     6241 aaccacgagt gatacaaggg tcagacgagt gtccgcaaaa atcatttccc tgtttgcccg
     6301 ggtaatagaa acaaataggg ctgcaggaag cgcgtagttt aataccagtt tattaaatgc
     6361 ccgggcttga tcttctgaaa aagtttctcg tctgccgcta aaataaccca acagcatgat
     6421 tacgataata ggcaataaat cgccaataaa aaatgttagc ataatagtat ttccgataag
     6481 ggtcccctca ccatcgcata atgagttggc aagaggagac gatgtcagta tttaattacc
     6541 agcgacttgt tttgggttaa gtttggtaat atggccactt tcagttcctg ctgcagggtc
     6601 gatgaccaca ttaataatgg tcggtttgcg cgactgaata ccggtggtta aagcatgacg
     6661 aagttcatct gtcgtggtga cgttatagcc aacgccacga aacgcatcca ttaatttgtc
     6721 ataccttgca tggtgcaaca gatccgttgg tgatggtgca ccagcgccac tgagatcaac
     6781 accgtctcct ctgtagatgc cgccattatt aaaaataacg atcgtcaccg gcaggttata
     6841 tcgacaaatc gtttcaatct ccatcccact gaaaccaaaa gcactatcac cttcaatggc
     6901 gacaaccgga gaaccagagg tcacgctagc accgatggca tagcccatac cgatgcccat
     6961 gacaccccag gtaccacaat ccagacgacg acgtggttta tacatatcaa taatatttcg
     7021 tgcattatcc agggtatttg caccttcatt aactaaataa atatcctggt tctcacgcaa
     7081 tacatcgcgc acagcactta atgcattaaa gtaatttaat ggttgggtat ctgtacttaa
     7141 tttttcatgc attttttgtg cattttgctg cttgtggata tttaaaatat cgcgccatac
     7201 cagtggagtc gtaaatgtgt tttgtttcag ctctgccagc ataccttgca tactggatgc
     7261 aatatcgcca acgactggca cagcaatagg gcggttgctg tcaatttcct gcggttcaat
     7321 atccagttga ataaactgtg tatctgccgc ccatcctttt ttaccgtgtg ccagtaacca
     7381 attcagtcgt gcaccaacaa gcatgacaac gtcagcattt gccagggcaa acgaacgcgc
     7441 agctgctgca gaaagtggat gcgtatcttc gaggatccct ttcgccatag acattggcag
     7501 gaatggaatc tgggcacttt caataaattc acgaagctgt tcgtcagctt gtgaatacgc
     7561 cgcgcctttg ccaaggataa ctaatggtcg ttcagctttt gctaaaagcg aaattgcgct
     7621 agcgactgac ttcgggcatg gcaataatgc tggcgacgga ttttcaactt ttacaatcgt
     7681 ggttaacgct tcgtcttttt ccatcgtcgc ggccaggaca tttgctggca aatcaagata
     7741 aactccgcca gggcgacccg atacggagac ccggatagcg cgtgccaatg caatgccaag
     7801 atcctgcggc tgattaacgc gaaatgctgc tttggcatac ggttttgccg cattcatttg
     7861 gtccagctct tcataatcac cttgctgtag gtcgacgatc gcgcggtcgc tggagccgct
     7921 aatcatgatc atcggaaaac catttaccgt tgcgttggcc aatgcggtca aaccattgag
     7981 gaatcctggc gcagaaactg tcaggcagat ccccggtttt tgggtaagaa aaccgcttgc
     8041 cgcagcggca tagcctgccg actgctcatg acgaaaacca atataacgaa tgccttccgc
     8101 ctgggcatgg cgtgccatat ccgtcacagg aatacctaca acaccataaa tagtgtcaat
     8161 attattctgt tttaatgctt caacgatgat atgcatacca tctgtcattt gaagttgatc
     8221 tgacatgatt aataacctcg aaatttttat aaattgggag ccagaagccc ccgttaatat
     8281 cagatggcgt ggttttgctt cattgcagca atttcatcgt cgctataacc cagctcctgc
     8341 aatacagcag cggtatgttc acctaatagc ggcgcagctt taatatccgg cgtaaaggca
     8401 gagaatttca ttggacaacc aacggtcaga tattttccac gcaacggttg ttccacttca
     8461 acaacactgc cactttggcg caaagaggga tcaagtgaaa tttctttcat acttaaaacc
     8521 ggtgcacaag gaatatcaaa ctgagtcaaa taggccaccg cttcatgttt atcaatagtg
     8581 acagtgtatt tttcgatttc agcaaaaata tcgaaaatat gtggctgtcg tgcatgggct
     8641 gtactgtatg ccggatcggt aatccattct ggtttgccga tggctttaca ggtgttttcc
     8701 cagttttgct cctgaatagt gaaataaata taggcgttag gatcggtttc ccagccttta
     8761 catttcagga tccagccagg ctgaccgcca ccacctgcat taccaccgcg gggaactgca
     8821 tcaccaaatg taccattcgg atactgcggg tattcttcca gataacccaa tttatcgaga
     8881 cgctgctggt cacgtaattt cacgcggcaa aggttcaata cggcatcctg cattgacatg
     8941 gtgactcgtt gcccacgccc cgttttttcg cgatgcagca aagcagcaag taaaccgatc
     9001 agcaaatgca ttccggtgtt gctgtcaccc aacgctgcag cgcttaccag cggcggacca
     9061 tcccaaaaac ccgtagtgga tgccgcgcca cccgctgcct gagcaacgtt ttcataggct
     9121 tttacattca cataaggcga acactcatca aaccctttga tcgaaccaaa aatcagacgt
     9181 ggattgattt cttgaatatg ctcccaggtg aagcccatgt gatcaatggc ccctggatga
     9241 aagttctcga ctaagatatc agcttcgcgg atcagctttt ccattacctc tttgccttcc
     9301 gctgttttgg tatttaactc aatagaacgt ttgttactgt taagcatggt gaagtaaagc
     9361 gcatcgatat caggaatatc tcgcagctgg tgacgcgtta cgtcaccaac gccgggacgt
     9421 tcaattttaa taacgtcagc gccaaaccag gccagcattt gagtacaaga tgggccagat
     9481 tgcacaccgg tgaaatcgag aactttaatt ccttgaagtg gagt
//
LOCUS       DAPGOF010000186         9485 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.187, whole genome shotgun sequence.
ACCESSION   DAPGOF010000186 DAPGOF010000000
VERSION     DAPGOF010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.187"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            236..748
                     /gene="trhF"
                     /locus_tag="RHV13_003998"
     CDS             236..748
                     /gene="trhF"
                     /locus_tag="RHV13_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncHI-type conjugal transfer protein TrhF"
                     /protein_id="HDV1243473.1"
                     /translation="MSDYIPKKRGLLILDWYVPINILLLILVMCVFFTRYTFGYGLLN
                     GCLPADFYMIDHSDKTIKTGELIAFNMPKSVRFIPENERVIKIVAGVGGDKLKVTMDG
                     VYNGDKFFEANARRISKKYSIPSILIEKELIIPEGEVFLIGQTDHSWDSRFWGTVKLN
                     SVIGKTYAIF"
     gene            735..2246
                     /locus_tag="RHV13_003999"
     CDS             735..2246
                     /locus_tag="RHV13_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001256132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDV1243474.1"
                     /translation="MRSFNVNKKYLPILPGLALCAVLSNTNASTEYQHDADLIAQQAK
                     GLGAQAKGAQQPDGALSLDATLNSPDVQKYIAQAEALKKNQDLSRQTNRGYVPGMNAD
                     SVQAVIDHTQAIRAQSNNSDAVNDIIRRRDEIQENASLNEAALKAVENKPEVMRGQSK
                     NIEKLFGSSGITAADFERKMDSTREEALSTENGITIFASFSLPDYVLEDLLRTASEHK
                     ARVVFNGLKKGTTRLPETQAAINQMIVKGKFESPLITIDPDSFSQYQVTQVPTIISRE
                     QARFAKMVGSFNVEFFQRELAKKPDQDIFPVAGTIYPVEEKSIIKELEERAQKYDWEG
                     AKKRAVADTWKNQWMVDLPPAQEHKEFLIDPTVRVTQDVKDKQGRVIASAGELINPLS
                     RFPQNLTMIIFDPLNPGQLVWAEQQYRQRLGSGKVMPMFTRIQKDNGWDHLNDLREKF
                     NGKVFKVNEQIISRFQIKNTPALITTDQDKFRITLFSEAEVRGIGAPNLSEEK"
     gene            2391..3446
                     /gene="trhU"
                     /locus_tag="RHV13_004000"
     CDS             2391..3446
                     /gene="trhU"
                     /locus_tag="RHV13_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncHI-type conjugal transfer protein TrhU"
                     /protein_id="HDV1243475.1"
                     /translation="MKKFKKLLLEFIVAVMLSLSIPGMAMAADAGVPGAMCQSAGVWQ
                     GLIKNICWSCIFPMRIMGIGAAPEGAAPSRPGCYCTDQNGVPEIGWQLSFFQPVKIVE
                     VVKSPWCSPFLEGTMLQKSQFDIGKSNTNQPMTATEAGFYDVHLWEFPIMTMLKLLII
                     GECTAEPYIDASLTYISEVDPMWESDLLTLVLNPEAVVFANPIASMVCAADCVAVTAG
                     KDNLAAYFCAGCDGNLYPLTGHIYANDDAVRTSSLITQRLLTKLHRQGMLMRTMGADA
                     MCEKTWEYFTPRSQYRLSMLFPTPEAKGPDCCHRLGDSVHDWSTLKGGRKKIGNDNYV
                     YMLWRYNDCCVRYVPGV"
     gene            3465..6653
                     /gene="trhN"
                     /locus_tag="RHV13_004001"
     CDS             3465..6653
                     /gene="trhN"
                     /locus_tag="RHV13_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncHI-type conjugal transfer protein TrhN"
                     /protein_id="HDV1243476.1"
                     /translation="MKLRKTITSAIIASMVANTMSWAFYVTLINMVMTPRVYAADAIF
                     DQLENNFNLANPNANRNATTSAQDIVEKYKNADSGENVSGKITEKYVGKAESANLNLG
                     KYGAGNSNESVMKNAASDGKSIGSAVQLPSMSGGTINSNYTKEGAKLLSRDANGNIGI
                     SSNPNTTPGTKTNTGEIFSSEQKHSDIKFNAADRYGDEDGFVNDIKNRKSQLFDAQSY
                     DGVAYRTLVNANKDNPASSVKPNDPMFTAGRNEITNAVAGTGNWLQNCNVETSKKTVT
                     THYPDYKEFYCNSPKQDNFSSCTITRDFSVPVYISGGNGDMSMCGDNCVRIWFGRRDD
                     NYWSDGVFDNELTLKFHPDAKLASAKIVNAEWDDHMRVTLDGTQIFAHIDGAYRESDY
                     PAPKGSWELKKSWKLDKVYDVTEKVRKSVYEEPDREVTMASRVWVGGGGEGYFEVELT
                     FENMKLEDKHVQEPAGCYDAVQTPNTFCRFDRFKDMDVGTKRLPESVLSMAKPLYEGD
                     KGFLTWKTNLEGYFCDPLAKDKICSYDASGKIMKDANGKDLCYNYEEIKSMPDACSSY
                     KNDAACVLDKQTCAEGWFDEGTNSCYMYEQKYTCDRGKDVVREVESSTNACVGMIPCS
                     GGTCETGPKEENNDFGKVAAYSNMVQYMQGEAKCEDPNDANSCSVFEGKPEWCGRSVG
                     FVNGLAKTDCCEAPQGTAGALEGIMLAGSMIRNTNWTRVNAQLIKWTGGDTGTWASMS
                     NAVGEWTASAGKTVGQMWNNVTSSLTSVYENVAGNLSRAVGSSATSGGAGGAGQLAQE
                     TMSSFGIGQLKQMAMKKAYELLPDTVRDFVFKNVATTGGEVVFSAAVQNFMLALNVIG
                     WIYTAYQVTKMLLEMLVACDQKEMEASIHKNQKSCFTLDTERCVKYLNVGFTKKCVKK
                     ATDMCCYNSMLSRVIMQQAYPQLGIDPVASNCVGLSIKQIQQLDFDKIDLTEWINDAV
                     QVGEVPDQYSKFSEESIVENLPFQNENYQLPSERTKEAMGGEENMIKARQENAQAIKE
                     ENVDCSYLPRPAICEVGSTTLDPVTGKQLPKY"
     gene            complement(6682..7554)
                     /locus_tag="RHV13_004002"
     CDS             complement(6682..7554)
                     /locus_tag="RHV13_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243477.1"
                     /translation="MKLLRLVSLVSSVVIGFSLSGCAGDTGTTPGTALNAYSHGQYWH
                     AKANDSVAKNAYSFAGFDVNFKEQEVTPGGDADNFLSKYLINSSMGYVTGGLKGFGIM
                     SLGSLYSSSDAEYIQVNQYLVFVPNPKKLPYNDESLVREGAKYVFEHTKATQASFGYN
                     PEKQKAALATCKIDMAVVNKWSTCDLPSKPDADVFPTDSMYSFQAIRPATGTEIPQLN
                     LPAGDYAVIRYVFIPFKGNETSADFSGIIFRSDSPNTTTPGGVAASINGKDYYLFTGE
                     YGKKGFPEKTLKAK"
     gene            7734..>9485
                     /gene="trhI"
                     /locus_tag="RHV13_004003"
     CDS             7734..>9485
                     /gene="trhI"
                     /locus_tag="RHV13_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncHI-type conjugal transfer helicase TrhI"
                     /protein_id="HDV1243478.1"
                     /translation="MGKPTEEQRPVIENASANNMVIAAPGSGKSFTMIEAVISILKKY
                     PYARIGMVTFTRAATNALAAKLQKRLSKKDLDRVLVDTFHGLVKKQLDMIRWPGKMLI
                     GPAQRSVIHRALKESGVTMKFAEAEFVIDAIGREMDTDVISVRHNRQQIHLFNTYQAL
                     CQKDHVADLNALSKFVVGQMHSGKMRTLDLTHLIVDEVQDTDSIQFSWIALHTRAGVY
                     TSIVGDDDQAIYSFRSSGGVKIFQQFEKHFRPNIFYLNTCFRCEPEILEVAGALIGKN
                     VYRYAKELRSAKKGGGKVTFRSYVDMEEQIQGILSLINQDPHGWAILSRNNAHLDELE
                     SLIEQPVIRYGGKSFWDEKETSDVLSLMAFFRQSNDPRLMKRVLALFGEQESVLDEVA
                     LSMRGRKVTFGDLAIPEDSSLETKTLHSNFVRFTQESTDKVEIAKRFANLTKWMESSS
                     IKMRSNKGTATLTKIALDTCKQWAEKTGWMNMINRAAAMSLGPRKKDEEYSPEKVVLS
                     TLHGSKGLEWNKVIIMSCNADQIPSKRSVGEEAIEEERRLLYVGFTRAEKQLFVMWYG
                     DPSFFLRECSEEKIKEAA"
BASE COUNT         2947 a         1825 c         2252 g         2461 t
ORIGIN      
        1 tttatgccca acaattggac ctttgaaaaa ttaataccgg aaagtaactt tgctacaaag
       61 gtatctttca tggccgctaa caaggctgcg tctacatgct gagaaattaa catccttata
      121 ggtgaatatt aggttagttt gcagacttcc gagcgttgaa aaacatggat aatagttact
      181 tggatagagg tgtatctact tagtaaaaat attcttcagt gccagtgaga aaaatatgag
      241 cgactacata cctaaaaaga gagggctgct tatactggat tggtatgtcc ccattaatat
      301 tttattgctg attctcgtta tgtgtgtctt tttcacccgt tatacatttg gatacggttt
      361 attgaatggg tgcctgcctg ctgattttta tatgattgat cattcggata aaactattaa
      421 aactggtgaa ctcatagctt tcaacatgcc gaaatcggtt cgttttatcc cagaaaatga
      481 acgagtcata aaaattgttg ccggtgtggg cggtgacaaa ctcaaagtaa caatggatgg
      541 ggtttataac ggggacaaat tttttgaagc taatgcacgt cgtatttcaa agaaatacag
      601 tattccgtcc attttgattg aaaaagaatt gataattcct gaaggcgaag ttttcctaat
      661 tgggcaaacc gatcactcat gggattcccg tttttgggga acagtaaagc tgaattcagt
      721 aattgggaaa acatatgcga tcttttaatg taaataaaaa atatttacca atacttcctg
      781 gacttgcgtt atgtgcagtt ttaagtaata caaatgcaag cactgagtat caacatgatg
      841 ctgacctaat cgctcagcaa gcaaaagggc ttggcgctca ggctaaaggt gcacagcagc
      901 cagatggggc tcttagcctt gatgctacgc taaattcacc agatgtgcag aagtacattg
      961 cgcaagctga agcactcaaa aaaaaccagg acctgagcag gcaaacaaac cgtggttacg
     1021 tgcccggtat gaatgcggat agtgttcaag cagttataga ccatacccag gctataaggg
     1081 cccaaagtaa taactctgat gctgtcaacg atataattag aagacgtgat gaaattcagg
     1141 aaaacgccag ccttaatgaa gcggctctga aagctgtgga aaataagccg gaagtcatgc
     1201 gaggacaatc taaaaatatc gaaaaattgt ttggttcctc gggcatcaca gctgctgact
     1261 tcgaaagaaa aatggatagc acacgtgaag aagcactatc aactgaaaat ggtataacga
     1321 tctttgcttc atttagctta ccggattatg tactggaaga ccttttacgt acggcctcag
     1381 agcataaagc tcgtgtagtt ttcaatggtc taaagaaagg aactactcgg ctaccagaga
     1441 cacaggcagc aataaaccaa atgattgtca aaggaaaatt tgaatctcct ttgattacaa
     1501 ttgatcctga tagcttcagt cagtatcaag taactcaagt accgacaatt atttctcggg
     1561 agcaagctcg ttttgcaaaa atggtagggt ccttcaacgt tgagtttttt cagcgagaac
     1621 ttgctaaaaa acccgatcag gacatttttc ctgttgctgg tacgatttat cccgttgaag
     1681 aaaagagcat cattaaagaa ctggaagaac gcgcacaaaa atatgactgg gaaggtgcaa
     1741 aaaaaagggc cgttgcggac acctggaaaa atcaatggat ggtcgatctc cctcctgccc
     1801 aggaacacaa agaattttta attgacccta cagtgagggt cactcaggac gtaaaagata
     1861 agcagggccg tgtaatagct tccgccggtg agttgattaa tccactttcg agatttcctc
     1921 aaaacctgac gatgataatt ttcgatccac tcaatccggg ccagctggta tgggctgagc
     1981 agcagtatcg acaacgtctg ggtagtggaa aagtaatgcc aatgtttacc cgtatccaaa
     2041 aagataatgg atgggatcac ctaaatgatt tacgtgaaaa atttaacggt aaggtgttta
     2101 aagtaaacga acagattatc tcacgctttc agattaaaaa tacacctgcg cttataacta
     2161 ctgaccaaga taaattccgg atcaccctgt ttagcgaggc agaagtccgt ggaatcggag
     2221 ccccaaatct ttcagaggaa aaataatatg aaactcggat ttattatagg cgctctcttt
     2281 gcaacatggc tgacgctaaa ttatccggac cagatgagaa gttattttac caaagccgtc
     2341 gactatatcg aaagtgttgc aacatttatt acatccaagt gagcatgatg gtgaaaaaat
     2401 tcaaaaagtt acttttagag ttcattgtag cagttatgct ttcgctttct atacctggta
     2461 tggctatggc agcagatgct ggagtccctg gggctatgtg tcagtcagct ggagtgtggc
     2521 agggcttgat taagaatatc tgttggagct gcatttttcc catgaggata atgggcatag
     2581 gggctgctcc agaaggcgct gcaccttcac gtccgggttg ctactgcaca gatcagaacg
     2641 gcgtaccgga aataggttgg caattgagct tctttcagcc ggtgaagatt gtagaagttg
     2701 taaagagccc ctggtgcagc ccctttcttg aaggcacgat gcttcaaaaa tcgcagtttg
     2761 atattggtaa aagcaacact aatcagccaa tgacagcaac tgaagccgga ttttacgatg
     2821 ttcatctttg ggagttccca atcatgacaa tgctcaaatt actgattatt ggcgagtgca
     2881 ctgctgaacc ctatatagat gccagcctga cctatataag cgaagtggac cctatgtggg
     2941 aaagtgatct gcttacactc gtcctgaatc ccgaggcagt agtttttgca aacccaattg
     3001 cctcaatggt gtgcgctgct gactgtgtag cagtaacagc aggaaaggat aatctcgctg
     3061 catacttttg tgccggatgc gatggcaacc tttacccatt aaccggccac atttacgcaa
     3121 atgatgacgc tgtaaggacg agttcactga ttacccagcg tcttctgaca aaactacatc
     3181 gccaaggcat gctgatgcga acgatgggag cagacgcaat gtgtgagaaa acgtgggaat
     3241 actttacacc acgttcccag tatcggcttt caatgctttt ccctactcct gaggcgaagg
     3301 gaccggattg ctgtcatcgt cttggtgatt cggtacacga ctggtcaacc cttaaaggtg
     3361 ggcgcaaaaa aataggcaac gataattatg tctatatgtt gtggcgttac aatgactgtt
     3421 gtgttcgcta cgttccaggc gtttgatttc taaggagaaa ataagtgaaa cttagaaaaa
     3481 caatcacctc agcgattatt gcatcaatgg ttgccaatac tatgagttgg gctttttatg
     3541 tcacactcat aaacatggta atgacaccac gtgtttacgc tgctgacgct atatttgacc
     3601 agctcgaaaa taattttaac cttgcaaatc ctaacgcaaa tcgcaatgca accaccagtg
     3661 ctcaggacat tgtggagaaa tacaagaacg cggattcagg cgagaatgtc agtgggaaga
     3721 tcaccgagaa atacgtgggt aaagccgaat ccgctaatct taatctcggg aagtatgggg
     3781 cagggaactc taatgaaagt gtcatgaaaa atgccgcatc cgatggaaag tctattggta
     3841 gcgccgtaca actgccgagt atgtctggag ggacaatcaa ttcaaactac accaaggaag
     3901 gggcaaagct gctctctcgt gatgctaatg gcaacattgg tataagcagt aatcccaata
     3961 ctactcctgg aaccaaaaca aatacgggag agattttcag ctcagagcaa aagcacagcg
     4021 atataaagtt caacgccgca gatcgttatg gggatgagga tggctttgtt aacgatataa
     4081 agaatcggaa aagccagtta tttgatgctc aaagttatga tggcgttgcc taccgcaccc
     4141 tcgtaaatgc taacaaggac aacccggcat cgtctgtaaa gcctaacgat cccatgttta
     4201 ctgcggggag aaatgagata accaatgctg ttgctggtac tggaaactgg cttcagaact
     4261 gtaatgttga gacctccaaa aaaacagtaa ccacacacta tcctgattac aaagagttct
     4321 actgtaactc gcctaagcaa gataacttca gcagctgcac gataactcgt gacttctctg
     4381 ttcctgtata catttcaggc ggaaacggag acatgtcgat gtgcggcgat aattgtgtgc
     4441 gtatatggtt tggtcgccgt gatgacaact actggagtga cggggtattt gataacgaac
     4501 tgacattaaa attccatccg gatgcaaaat tggcctcagc aaagattgtt aacgcggagt
     4561 gggatgacca catgcgggta acacttgatg gcacgcagat ttttgctcac atagatggtg
     4621 catatcgaga aagtgactat ccggctccaa agggatcttg ggagttaaaa aaatcgtgga
     4681 aactcgacaa agtatacgat gtaactgaaa aggtcagaaa atcagtttat gaagaacctg
     4741 atcgtgaagt gacaatggca tccagagtct gggttggtgg gggaggtgaa gggtactttg
     4801 aggttgagct gacgtttgaa aatatgaagc tcgaagataa gcacgtccag gaaccagctg
     4861 gttgttacga tgctgtacaa acaccaaata cattctgccg tttcgatcgg tttaaagata
     4921 tggatgttgg cacgaagcgg cttccggaaa gcgtgctttc tatggcaaag ccgctgtatg
     4981 aaggcgataa aggctttctt acctggaaga ctaaccttga aggctatttt tgtgatccat
     5041 tggctaaaga caagatttgc tcttatgacg ctagtgggaa aattatgaaa gacgctaatg
     5101 gaaaggacct ctgttacaac tacgaagaaa ttaagagtat gcctgatgca tgtagttctt
     5161 ataaaaatga tgcagcatgt gtactcgata aacaaacatg tgcagagggt tggttcgatg
     5221 agggcactaa cagctgctac atgtatgaac agaaatacac ctgtgacaga ggtaaggatg
     5281 tagttcgtga ggttgaatct tctacaaatg cctgtgttgg catgatccca tgttctggag
     5341 gaacatgcga aactgggcca aaggaagaga acaacgattt tggaaaagtt gcggcttatt
     5401 caaacatggt tcagtacatg cagggcgaag ccaaatgcga agaccctaac gatgccaatt
     5461 cctgttctgt ctttgaagga aaacctgaat ggtgtggtcg ttcagtaggc tttgtaaatg
     5521 gcctggcaaa gacggactgc tgcgaagcac cgcagggaac cgcaggagca cttgaaggta
     5581 tcatgcttgc cggatctatg atcagaaaca ctaactggac cagagttaat gctcagctaa
     5641 taaaatggac cggcggtgat acaggtacat gggcttctat gtccaatgcg gtcggtgagt
     5701 ggactgcatc agctggtaaa acagtagggc aaatgtggaa caatgtcacg agctccttaa
     5761 cgagcgtata tgaaaacgtc gctggtaatc taagcagggc agttgggtct tccgcaactt
     5821 ctggaggggc aggaggtgct ggccagttgg cacaggaaac aatgtccagc ttcggtatag
     5881 gtcagcttaa gcagatggca atgaagaaag cctacgaatt gctaccagat acagttcgag
     5941 actttgtctt taagaacgtt gctactaccg gaggagaggt tgtcttctcg gctgcggttc
     6001 aaaactttat gcttgcatta aacgtgatcg gttggattta tacagcctat caggtcacta
     6061 aaatgctttt agaaatgttg gtagcatgcg accagaaaga gatggaagct tctattcata
     6121 agaatcaaaa atcctgtttt acattagata cggaacgatg cgtcaaatat ctgaatgttg
     6181 gtttcacaaa gaaatgcgtt aaaaaagcaa cagacatgtg ctgttataat tccatgttgt
     6241 cacgcgttat tatgcaacaa gcttatccgc aattaggaat agaccctgtc gcctctaatt
     6301 gcgtggggct atcaataaag caaatacagc aactggattt cgataaaatt gatctgacgg
     6361 agtggattaa tgatgcagtt caggtaggag aagttcctga tcaatattca aaattctccg
     6421 aagaatcaat tgtggaaaac ttaccattcc agaatgagaa ctatcaactt ccatcagaac
     6481 gtaccaagga agcaatgggc ggtgaagaaa atatgataaa ggccagacaa gaaaatgctc
     6541 aggctataaa agaggaaaat gtagattgta gctatttacc tcgaccggcc atatgtgagg
     6601 taggttcaac tacgctggat ccggttacgg gcaagcagtt gccgaaatat tgaagaaggg
     6661 gccgttaggc ccctttttta attacttggc tttcaatgtt ttttctggga agcctttttt
     6721 accgtattca ccggtaaaga ggtaataatc ttttccgttg atggaggctg caacaccccc
     6781 tggagttgtt gtatttgggc tatcagaacg gaaaatgata cccgaaaaat cagcgcttgt
     6841 ttcgtttcct ttaaatggga tgaacacata acgaattaca gcgtaatcac cagcgggaag
     6901 gtttagctga ggaatttcgg ttcctgttgc ggggcgaatc gcctgaaagc tgtacattga
     6961 atctgtaggg aaaacatcag catcaggctt agagggcaga tcacaggtac tccatttatt
     7021 gacgactgcc atatcaattt tacatgtggc cagagctgct ttctgttttt cagggttgta
     7081 tccaaatgag gcctgagttg cttttgtatg ctcaaaaaca tacttagctc cttcacgtac
     7141 caaactttca tcattgtaag gcaatttttt tggattgggg acgaacacta ggtattgatt
     7201 gacctggatg tattcggcgt cagaagaact gtacaaagag ccgaggctca tgatcccaaa
     7261 accttttaga ccgcctgtta cgtagcccat cgaactattg atgagatact tagaaagaaa
     7321 gttgtcggca tcccctccag gagtaacctc ttgctcctta aaattaacgt cgaaaccagc
     7381 aaacgaatag gcattcttgg cgacagaatc atttgcttta gcatgccagt actggccgtg
     7441 actgtacgca ttcagagcgg tgcctggcgt agtgccggtg tcaccggcac aacccgatag
     7501 ggaaaaacca atgacgacag aagagaccag agatactaaa cgtaaaagtt tcatttaaag
     7561 ccctttttgt aatgtctttt ggagatggaa ccattattcc atagtcacgc gtttcacaaa
     7621 caacctgatt gcataaaaaa ggaaaaaaag tgatttccag tagaaccagc agtacacaag
     7681 aaaagcgctt ttataaaaat gtataattat taaaacgaag taatgaggtt gaaatgggta
     7741 agccaacaga agagcaaaga cctgttatcg agaatgctag cgcaaacaat atggtgatag
     7801 cagcgcctgg atcaggcaaa tcattcacca tgatagaagc ggttatatcg attctcaaaa
     7861 agtatccata tgcaagaatc gggatggtga cctttactcg cgctgcaaca aacgcacttg
     7921 cagcaaagtt acaaaagcga ttgagcaaga aggatcttga ccgggtgctg gtggatactt
     7981 ttcacggact cgttaagaag caactggaca tgattcgctg gccgggaaag atgttgatag
     8041 ggccagcaca gagatctgtc attcaccggg ctttgaaaga atccggtgta acgatgaagt
     8101 ttgctgaagc tgaattcgtt attgatgcta tcggcagaga aatggatacg gatgtgattt
     8161 cagttcggca taacagacaa cagatccatt tatttaatac ctatcaggcg ttatgccaga
     8221 aagaccatgt ggcggacctc aatgctcttt caaaatttgt tgtcggtcaa atgcattcgg
     8281 gaaaaatgcg cacgttagat ttgacacatt tgatagtaga cgaagttcag gatactgact
     8341 ctatacaatt ctcatggata gcattgcata caagagctgg agtctatacg agtatagttg
     8401 gagacgatga tcaggccatc tactccttca gatcctcagg aggagtcaaa atatttcaac
     8461 agtttgaaaa acacttcaga cctaacatat tttacttaaa cacatgcttt aggtgtgaac
     8521 ctgaaatttt agaggtggcc ggagcactga taggaaagaa tgtttaccgt tatgccaaag
     8581 agttacgctc ggcaaaaaag ggaggaggaa aggtcacttt tcgttcatat gtagatatgg
     8641 aggaacagat acagggtatt ttaagcctta taaatcagga tccacatgga tgggcaattc
     8701 taagccgcaa taatgctcac ttggatgagc tagaaagttt gatcgaacag ccagtaattc
     8761 gttacggagg taaatcattc tgggatgaaa aagaaacaag cgatgtgtta agccttatgg
     8821 cctttttccg acaatcaaat gatcctcgct taatgaagag agtattggca ttgtttggag
     8881 aacaggaatc agttttggat gaagtggcat tgagtatgag aggaagaaaa gttacttttg
     8941 gcgaccttgc catcccggag gacagttctc tcgaaacaaa aacattacat tcaaacttcg
     9001 tgcgctttac ccaggaaagt actgataaag ttgagatagc aaaacgtttc gccaacctta
     9061 cgaaatggat ggaatcgtcg tcaatcaaga tgaggagtaa taaaggtaca gcaaccttaa
     9121 caaaaattgc tttagacact tgtaaacagt gggcagaaaa gactggttgg atgaatatga
     9181 tcaatcgtgc tgctgcaatg tcattaggcc ctaggaagaa ggacgaggaa tacagcccag
     9241 agaaagtagt cctatcaacc ttgcatggtt caaagggtct tgagtggaat aaagtaataa
     9301 taatgagttg taatgcggat cagataccaa gtaaaagatc agttggggag gaggcaatag
     9361 aagaagaaag acgtttgctt tatgttggat ttacgcgtgc agaaaagcaa ctttttgtaa
     9421 tgtggtatgg tgacccttct ttctttttaa gagaatgcag tgaagaaaag ataaaagaag
     9481 cagct
//
LOCUS       DAPGOF010000187         9365 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.188, whole genome shotgun sequence.
ACCESSION   DAPGOF010000187 DAPGOF010000000
VERSION     DAPGOF010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.188"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..216
                     /locus_tag="RHV13_004004"
     CDS             <1..216
                     /locus_tag="RHV13_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243479.1"
                     /translation="KWNSQEVQDEVAALNQKIADDDNKIRSNLNIVQNFRRENQYKAN
                     KKISDAMRNILDWFERYPDIVKNITQA"
     gene            complement(353..1018)
                     /gene="yieH"
                     /locus_tag="RHV13_004005"
     CDS             complement(353..1018)
                     /gene="yieH"
                     /locus_tag="RHV13_004005"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HDV1243480.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGINVDLEEIFT
                     RFKGVKLYEIIDIISKEQGVTMVQADAEHVYRAEVARLFDTELEDIAGANELLASINV
                     PMCVVSNGPVSKMQHSLGKLNMLHYFPEKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSSAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTELAQLPALWKARGWN
                     ITR"
     gene            1185..2522
                     /gene="adeP"
                     /locus_tag="RHV13_004006"
     CDS             1185..2522
                     /gene="adeP"
                     /locus_tag="RHV13_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HDV1243481.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            complement(2576..3142)
                     /gene="yieF"
                     /locus_tag="RHV13_004007"
                     /gene_synonym="chrR"
     CDS             complement(2576..3142)
                     /gene="yieF"
                     /locus_tag="RHV13_004007"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HDV1243482.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPVSMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILIFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(3164..3913)
                     /locus_tag="RHV13_004008"
     CDS             complement(3164..3913)
                     /locus_tag="RHV13_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HDV1243483.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTSSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTMKAFSPN"
     gene            complement(4070..5029)
                     /gene="yidZ"
                     /locus_tag="RHV13_004009"
     CDS             complement(4070..5029)
                     /gene="yidZ"
                     /locus_tag="RHV13_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HDV1243484.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLAALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            complement(5004..6179)
                     /gene="mdtL"
                     /locus_tag="RHV13_004010"
     CDS             complement(5004..6179)
                     /gene="mdtL"
                     /locus_tag="RHV13_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HDV1243485.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMAIMGIAVLMLSLFILKETRPAAPAASDKSRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLTVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHS"
     gene            complement(6311..7558)
                     /gene="tnaB"
                     /locus_tag="RHV13_004011"
     CDS             complement(6311..7558)
                     /gene="tnaB"
                     /locus_tag="RHV13_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HDV1243486.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSSTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGIIVILCWFGNVFNVLPKFG"
     gene            complement(7649..9064)
                     /gene="tnaA"
                     /locus_tag="RHV13_004012"
     CDS             complement(7649..9064)
                     /gene="tnaA"
                     /locus_tag="RHV13_004012"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HDV1243487.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            complement(9285..9359)
                     /gene="tnaC"
                     /locus_tag="RHV13_004013"
     CDS             complement(9285..9359)
                     /gene="tnaC"
                     /locus_tag="RHV13_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HDV1243488.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
BASE COUNT         2406 a         2320 c         2334 g         2305 t
ORIGIN      
        1 aagtggaata gtcaagaagt acaggatgaa gtagcggcat taaatcagaa gattgctgac
       61 gatgacaata agatacgttc taaccttaat attgttcaaa attttagacg cgagaatcaa
      121 tataaagcta ataaaaaaat cagtgacgcg atgcgtaata ttctggattg gttcgaacgg
      181 tatcccgata ttgtgaaaaa tataacccag gcttaaccat acagagggtg agcgactgga
      241 cgattaacga cataaattat ttatagggaa tagacgctta ctcatcagac acctggtatt
      301 ttttatctca aaccagcgtg actgatgtgt aagcgttgaa gtaaggaggc gattatcgcg
      361 ttatattcca gccccgtgcc ttccagagtg caggcagctg cgccagctcg gtaaaggtgg
      421 tgactttcgg gtgaacgatc ggtttattgt gcgggtcagc gcagaagtaa aacacttcca
      481 ttccggcatc tatccctgat tgagcgccgg cgctggagtc atcgaccaga atgcagtttt
      541 caacgttcac attcatcgct tttgccgcat ggaacattaa tgccggatcg ggcttccaac
      601 gctggatatc gtagccgctg aacagttttt ccggaaagta gtgcagcatg ttgagtttac
      661 caagcgaatg ctgcattttg ctgaccggac cgttagagac gacgcacatc ggcacgttaa
      721 tgctcgccag caattcgttc gctcctgcga tgtcctcaag ttcggtatca aacagacgtg
      781 ctacttctgc gcggtaaacg tgctcggcat cggcctggac catcgtcacg ccctgttctt
      841 tactgatgat gtcgatgatt tcgtagagtt ttacgccttt aaaacgggta aaaatctcct
      901 ccaggtcaac gttaatgcca aattccctga acatcgtgac ataggcgcga gagcaaatga
      961 cctcgctatc gaccagcgta ccgtcgcagt cgaaaaatac cgcttctatc tgggacatgt
     1021 ctttccttta tcgttagttt aacgttttcg tcatgccaat tcgcatgacg caatcgttgc
     1081 cgtataagca aatacaatga aaaaaagtta tcttcagccg tccatattgt ctcattttgg
     1141 tatacgatag cgacggattt tccctccttg ttcggaaata gataatgagt caacaacaca
     1201 caacccaggc ttctggccag gggatgctgg aacgcgtgtt taaactgcgc gaacatggca
     1261 cgacggcacg gaccgaagtg atcgccggtt ttaccacctt cctgacgatg gtttacatcg
     1321 tttttgttaa cccgcaaatt ctcggcgttg ctggcatgga caccagcgcc gtcttcgtca
     1381 ctacctgtct gattgctgca ttcggcagta ttatgatggg actgtttgct aacctgccag
     1441 ttgcactggc acccgctatg ggcctgaatg cgttcttcgc ttttgtggtt gtgcaggcga
     1501 tgggcttgcc gtggcaagtc gggatgggcg caatcttctg gggcgcgatt ggtctgctgt
     1561 tactgacgat tttccgcgtt cgctactgga tgattgccaa cattccggta agtctgcgtg
     1621 tgggcatcac cagcggtatc ggtctgttca ttggtatgat gggactgaag aatgcaggtg
     1681 tgattgtcgc taacccggaa acgctggtca gcatcggtaa cctgacttct cacagcgtgc
     1741 tgctggggat cctcggcttc ttcatcatcg ccattctggc ctcgcgcaac attcacgcgg
     1801 cggtgctggt ttccatcgtg gtgacaacgc tgctgggctg gatgctgggt gatgtccact
     1861 ataatggcat cgtttctgcg ccgccgagcg taatgactgt cgtgggtcat gtagatttag
     1921 ccgggtcgtt taacctcggg ctggcagggg tgattttctc tttcatgctg gtcaacttgt
     1981 ttgactcctc tggtacgctg attggcgtga ccgataaagc aggtctggcg gatgagaaag
     2041 ggaaattccc gcgcatgaag caggcgctgt atgtcgacag tatctcttcc gtgaccggtt
     2101 cttttatcgg tacttcttcc gttacggcgt atattgagtc ttcttccggt gtatcggttg
     2161 gcggtcgtac cggtctgacg gcagtggttg ttggcctgct gttcctgctg gttatcttcc
     2221 tgtcgccgct ggcgggaatg gtgccaggct acgctgcagc tggcgcgctg atttacgttg
     2281 gcgtgttgat gacctcaagt cttgctcgcg tgaactggca ggatcttact gaatctgttc
     2341 cggcgtttat taccgctgtg atgatgccgt tcagcttctc gattaccgaa ggtattgcgc
     2401 tgggctttat ctcctactgt gtgatgaaaa tcggtaccgg gcgtctgcgt gaccttagcc
     2461 cgtgcgtaat catcgttgcg ctgctgttta tcctgaagat tgtgtttatc gacgctcact
     2521 aaaatcgata gcgcgttaaa aacccgccat gcatgattgc tggcgggttt tttatttaga
     2581 tcttaactcg ctgaataaac tcaccaaatg cggtcaattg cccggtcagg tggtccagcg
     2641 taccctgatc aatcacttct ccggtttgcg gatcaacttt gttctgaatc acgccgccca
     2701 taaattccgg cttgttcatc accattgcat cgagaaaaat cagaatctga cgcaggtgat
     2761 actgacagcg cgcgccacca atcacgccca ttgagctggt ctgaatcaat accggtttac
     2821 cagccagcgg ttgatccggc aggcgggaaa gccagtcgat ggcatttttc agcccacccg
     2881 gtaccgagta gttatattcc ggcgtgacga tcaccacacc gtcagcctga cggatttgtt
     2941 ccgccagagc ttcaaccgtt gctggaaaac cttcttcctg ctgtacgtca gcgtcataaa
     3001 ggggaatgtc ggcaatggaa ggtaacgcat tgacttccat gctcaccgga gcaattttcg
     3061 gcagggtacg tgcaaccatg ccattaaatg agcctttgcg caggcttccc agtaacgtaa
     3121 ccacctgcaa tttttcagac ataatgactc ctgtggttgt ctatcaattt ggcgaaaaag
     3181 ccttcatggt agagagctgg gcaaaacgca tcatccggct ggtaggattg ttggcgcggc
     3241 tgtgaatatc cggcaggctt ttccagccgt gtttgccatc taactcccag acactgagct
     3301 tttcaatcgt gggcgtgacc gccacggacc acaccagcac gtcctccgcg tcgcataacg
     3361 cttctacatg atttacatga gcacatttca ggcgtccggc aatgggcagc agttgcagat
     3421 cgcgcggatc gtcattcaaa acatcaccgg ttagttttag cacgcttcgg cgcagcgtga
     3481 tgagctgcgc ccgcgcttcg tttggatcat tttgtttact gatccatagc gattcattgc
     3541 tggaagatgt gtggttatcg ggcgcgtgtg ggctatgaaa cccgcgcgtc gcacgctgca
     3601 gttccatatc aaggccacat tcaccttcgg ttgttaacgc cacgccgacc atattcccgg
     3661 cataggaaat agaaaacgaa ggcaaatttt tatcactgaa aaccggttta cctttcggca
     3721 gggtgacgat ttccggcaat tcgccaatgc catacagcat gaacatcagt tctgcgagta
     3781 aacctctgga cgccagaaaa cggctttgac gatgaggggg aatgtttcgg gcttcttgat
     3841 gacactgaga ggggagacga acagaaatca gatgtccttc cgttaaaatc cctcgggcaa
     3901 agtgagtcgc catttttcgc tccatgctta atggtccatt atgaatacaa gagtaatcat
     3961 ttactggaat gatggacaga ctaaagcggc taaaagttcg attcagataa gtttattaca
     4021 acttttttac attttattgt gaaatagggt cgcgtttcat aagattcggt tatgccatcg
     4081 acgcgtaaag gtttttaatg gtttcccgta accagacgat cttcggatta tggctgttcc
     4141 gtttatgcca cagcagggta aaagggactt ccagcttttt ttgctggctt tcgtcaaacg
     4201 ggagaggaag agcagccaac ggcagttggt ggagttgatt gtaatactgg cagtagcgcg
     4261 gcgcggtcgc cagtagcaaa ttgtcgggct gcgctgccat aaacagcgac tgctcgaatt
     4321 ccggcaggct catagcaatc gtgcgttcgc gccccagctc ctgtaacaca ttgtccagcg
     4381 cccaggtatc gctctgttcc cagcaaatgc tgatatgcgg ataacgtaaa aaggtgtcca
     4441 gattccacgt ttcatgcagt gccggatgat ctttgcgtaa ccagacgcaa ggcacatcac
     4501 taaacagcac ttcataatca atggctaacg gtagcgagct taacagctcc cgcgagcgag
     4561 gatggctttc gcgaccggaa aaaccgatat ccacttcacc acgagtaatg gcatctaagg
     4621 aatcgtaatc ccagttacgt aatttgatgg tcgcctgcgg gtaacgttgg tagatccgtt
     4681 tcgacagcgc attaagcatg atcatcatta gcggtgattc tgccgccagc tcaaacttca
     4741 ggccgcgcgg tgtctggtgg tgcggtttat ccagcagcag gttgctcatt tgcatccatt
     4801 ccgccagatt ttgctccatg ctgaccatta gcggtgtggg cgacagaccc agcggtgagt
     4861 tcacaaagag cgggtcgtca aaccacgctc ttaactttgc cagcgactta ctcaccgccg
     4921 aaggggtcac gttcatccgc ttcgccgctt tggttacgct gcgctcctgc atcagcagtt
     4981 gcagacagag cagcagattg agatcaagag tggtgatgga tttcttcatg agcggcaacc
     5041 gggcgtccag gcgcgacgaa cataatcagc aacaggctca ctatgctaca ggcaatcaga
     5101 atcccgatca gcatattcca tgcgccgata ccaaccaccg ccgccagcca aatccacagt
     5161 gacgaaccgc aaacctgcgc aatacctaag gtcgagctgg ctacgcccgc gcgtaatgaa
     5221 aacggcccta acgcctgact catcgccaca ccaaaaccta ccgagaaacc ggcgcaaatc
     5281 agcgtgatac caaacagaga aaccgcatgg gaaggtgaaa cggtaagagt gatgcccgcc
     5341 gccaggaata acacctgtga ggtgatcatc aacgtacgtg gcttaaaaat tcccagcgca
     5401 aatggcgtgg aaaatgaaac ggtcatgctg acgccagcgg tcaacgccat aatggtggcg
     5461 tattctccgc gctcaaaacc catgatttcc atcagcaata ccggcgacgt gttgacgaag
     5521 gtgaggatca ccgaaacgct gagggtggtg ataacaacac ggctgaggaa aaaccgatta
     5581 agcagcgact cgctattttc tcgtgatttg tccgaagctg cgggggcggc agggcgcgtt
     5641 tcttttaaaa taaacaaaga caacatcagt accgcgatgc ccattattgc catcgtccag
     5701 aacagactct gccacgggaa tttaagcata atcagatgac cgagcaccgg cgctaacacc
     5761 ggaatgatgc aggtaatgcc gttgagtaac gacagcactt ttgcccgacg tcgatcatcc
     5821 agcgtgtcgc gcaaaatggc gaatgccacc acgtaacaac agcccgcgcc caacccctgt
     5881 agaaagcgcc ctgcaagaaa taacgtgctg gtttcagcca gtgaacagaa caccgaggcg
     5941 ataataaata gcgccgcgcc tggtatggcg acaggctttc tccctgaacg atcggccact
     6001 ttaccggcaa ataacatcgc agctgccata cctgccagat aaacggagaa cgcaatatgt
     6061 aactgcgctt cgctggcatt gagatcggcg gcgatgcgcg gtaaaccaac gaggtacata
     6121 tcaatcccag cgggataaag taataccagg gcaaaactac aaatcaaaaa gcgggacatg
     6181 ggctaaagcg tcctgatagt aaggtgacgc aaagcatacg tggtgaagga ggatcaggcg
     6241 agttgccatt tggacataag tgatttcctg agaggcaaga agccagcgaa tggctggctt
     6301 cttgaaggat ttagccaaat ttaggtaaca cgttaaagac gttgccgaac cagcataaaa
     6361 ttacgattat accaaagaga atgacaatcg ccggaataag attgccgccc cagaccgtga
     6421 acatctgatt gggaaacttc ttgcgagctt tgattgccag caccgcgggg ataatgaccg
     6481 cccagatggt ggcgcacagt ccggcaccgc cgatcccgta aatgaagccg ttcgggaaga
     6541 tcagatacaa caacgcgggt ggcaggaagg ttaacagcac ggttttgaaa cgcccgccgt
     6601 gggagttatc aatcttaaac agatccgcca gataatcgaa caatcccagc gtgacaccaa
     6661 aaaacgagct ggcgacagct aagttggaga acaccagcag gcaaaactcg ataatgccgt
     6721 gctgtttggt gccgaggaac gatttcacca gtgaatcaac gttgccgcct gaggaaataa
     6781 tcgccttaaa gctttctcgc ggaatattcc ccatggtgca atagagccag aagagataaa
     6841 tcaccagcgc cagcagcgaa ccgaagacca cgcttttgat tagcttatct ttgcgtttgc
     6901 catagcaaat aatcaggctg ggaatattgc cgtggaaacc aaatgacgcc agacacaccg
     6961 gcaaagccat aaagatatac gggaagtaag acgttcccgc agtggagctg gtggcatcgc
     7021 gcagaatgga gtaatcgacc tggaagaaga aagaaccaaa cacgatcaca aaggagataa
     7081 tcttcagccc gaggaacaat gaggtaatac gactggcggc taacgagctt atccacaata
     7141 cgctggcaac gaaaatggct gtgcagatcc cgacaatacg tggattagcg tgataaccca
     7201 aattcattga tatcgtttca ctaatgatcg caccattagc agagatatag gcataagtga
     7261 ggatatagag aacaaaggca acggtaatac cgctgataat gttccaggtg ttaccgatta
     7321 aatctttggt gatggtgtta aaactggagc cgacgggata atttaaattt gcttctaata
     7381 acaataaccc ggaatgaagc attgaaaacc aggcaatgat aaggataaag gcaccccaga
     7441 aaaaccaggc accggcaaga tcaacaggta aagcaaacat acctccaccg attactgtac
     7501 ctgctataac cataacaccc caaaatgcag agtgcttttt ttcagcttga tcagtcatga
     7561 tgccaccttt agaggaaggc tatttttgtt attgaggatg tagggtaaga gagtggctaa
     7621 catccttata gccactctgt agtattaatt aaacttcttt cagttttgcg gtgaagtgac
     7681 gcaatacttt cggttcgtag gtaaaggtta accctttaat attcgccgcg ttctctttca
     7741 catgtttgaa ggcttcaata atgaagtcca tatgtgtttg agtatatgtt gcgcgcggaa
     7801 tggttaaacg cagtagttct gccgggcatg gcagttgttt accggttttc ggatcgcggc
     7861 ctaacaggaa agagccaatt tctaccgcac ggataccggc gactttatac agctcgcagg
     7921 ccagcgcctg tgccgggaac tgatctgccg ggatatgcgg caacagttta ccggcatcaa
     7981 caaatgccgc gtgaccgccc gcctgctggc agacaacgcc aatctcttcc agaccatcga
     8041 ccagatactg cacctgcgca atacgataag ccagccagtc cagattcatg ccgtcataca
     8101 gacctacggc cagacgctcc atcgcgccgc cttccaggcc gccatacgtc gggaagcctt
     8161 cctgaactac gcaaagggtt ctgcactcgg tgtacacatc aaagaaactg tcgtctttca
     8221 tgcacagcaa gccgcccatc ggcaccatcg catctttctt ggcggacatc gccagcatat
     8281 cggcatattt gtaggtttcg cgggtgatct gctcgatggt ccagtccttg tattctgctt
     8341 cacgctgctt gatgaaatag gcgttttcag caaagcgtgc ggagtccatt accaccggaa
     8401 tatcgtattt cttcgcgatg ctgtacatcg cttttaagtt tgccagtgaa accggctgac
     8461 cacctgcaga gttactggtg atggtcgcaa cgatatacgg cacgttattc ggcccaactt
     8521 cttcaatgcc gcgttctaat ccctcaaggt caaagttacc tttaaagtcg taacgcacgc
     8581 cggtatcgaa ggcttctttg atatagacgt tacgcacggt acagccgtta atctggctat
     8641 ggccctgcgt ggtatcaaag aaatagttag agaacgccac cattttgctg cgatccaggc
     8701 ctttttcctg ctcgcgcttt ttaatcagta ccggaatata aatttgctct gcaccacggc
     8761 cctggtgagt cggaatggtg tattgataac caaagatatt tttcactgac tcggctaacg
     8821 catagtagct gcggctgccg ctgtaagctt cgtcgccgcg catcatcgcg gcctgcatac
     8881 tctgcgtcac cgccccggtg ccgctgtcgg tcagtaaatc gataaacaca tcttcgctat
     8941 ccagcaggaa cgggttcata ccggatttaa taattgcctc ttcacgataa gcgcgagtgg
     9001 tacgttttac tggctcaata acacgaatgc ggaacggttc agggagatgt ttaaagtttt
     9061 ccattacata atccttcatt tattttaatt acagtgatcc ctgtgaatat tgcatctgca
     9121 atatccatta attcgccatg tatatatgca agcaccatca tttactaaca caagtaaata
     9181 aacgagagat ggcctttgca aaaggcaaat ttaagatata gcaaacaaat agatcacatt
     9241 gaatctaatc ggtttggctc tggtgatggc tacagaaggg caaatcaagg gcggtgatcg
     9301 acaatcttgt tgtcaatatt gaaccatttt gaggtcacac atatatgtaa gatattcata
     9361 atgca
//
LOCUS       DAPGOF010000188         9325 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.189, whole genome shotgun sequence.
ACCESSION   DAPGOF010000188 DAPGOF010000000
VERSION     DAPGOF010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.189"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(229..336)
                     /locus_tag="RHV13_004014"
     CDS             complement(229..336)
                     /locus_tag="RHV13_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic polypeptide LdrB"
                     /protein_id="HDV1243489.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(741..1841)
                     /gene="chaA"
                     /locus_tag="RHV13_004015"
     CDS             complement(741..1841)
                     /gene="chaA"
                     /locus_tag="RHV13_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HDV1243490.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            2111..2341
                     /gene="chaB"
                     /locus_tag="RHV13_004016"
     CDS             2111..2341
                     /gene="chaB"
                     /locus_tag="RHV13_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HDV1243491.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            2499..3194
                     /gene="chaC"
                     /locus_tag="RHV13_004017"
     CDS             2499..3194
                     /gene="chaC"
                     /locus_tag="RHV13_004017"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HDV1243492.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTALQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENFPDGVLRPGFA"
     gene            complement(3238..3591)
                     /gene="ychN"
                     /locus_tag="RHV13_004018"
     CDS             complement(3238..3591)
                     /gene="ychN"
                     /locus_tag="RHV13_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HDV1243493.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            3777..5171
                     /gene="ychO"
                     /locus_tag="RHV13_004019"
     CDS             3777..5171
                     /gene="ychO"
                     /locus_tag="RHV13_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HDV1243494.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGSWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(5172..5822)
                     /gene="narL"
                     /locus_tag="RHV13_004020"
     CDS             complement(5172..5822)
                     /gene="narL"
                     /locus_tag="RHV13_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HDV1243495.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(5815..7611)
                     /gene="narX"
                     /locus_tag="RHV13_004021"
     CDS             complement(5815..7611)
                     /gene="narX"
                     /locus_tag="RHV13_004021"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HDV1243496.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            7950..>9325
                     /gene="narK"
                     /locus_tag="RHV13_004022"
     CDS             7950..>9325
                     /gene="narK"
                     /locus_tag="RHV13_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HDV1243497.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGR"
BASE COUNT         2244 a         2289 c         2417 g         2375 t
ORIGIN      
        1 ctcaattcat cttcacttca tccctgaaat gtttgcaatg aagagtgcat tccggttttt
       61 caacagctgt tacagtcgtt tcatgagtgc tctggatgat gcttccagct cgggttgcca
      121 atatttactt gtggaagtga taaagacaaa aatggccgca ggctgttacc cctgcggccg
      181 gtttcgggcg catattgcca tcacggcagc ctgacgcccg ttttcacctt acttccggtt
      241 acgccaccag ccgacaatcg ctgcggtaat aattcccgcc aggatcggtg ccgccaggtc
      301 gtgccagaaa gtcatggcaa actgcgcgag cgtcatatag ccgccttgtt gtaatgacaa
      361 cattttgcgg ctattcttga agtgtctggt ttcaagatta gcccccgttt tgttgtcagg
      421 ttttacctct caacgtgcgg gggttttctc tttccagcaa ccaatgccac cagggataaa
      481 gcccccgcaa cattgcgcct caccggataa cgccggcttg gtgtggatac tacgtctcaa
      541 ttcatcttca cttcatccct gaaatgtttg caataaagag tacattccgg cttttcaaca
      601 gctgttgcag tggtttcgtg caggttgtgg tacaggctcg caattctgat taacgacctg
      661 tccaggtagt atgaagaaat acaataagca ggaatttata ttatctcccc cgatttccgt
      721 catcggagga gatataactg tcaggcaaat atcgtcatca aataggcggc aaacagtgcc
      781 agatgcgctg cgccattgag cacgttagta cgtccggtgg agaaggagat atggcacagc
      841 actaaagagg ccaccatcac caccatttct ggcgcaccaa gtgcaaactg caattcgtta
      901 cccgtcataa atgcaattag cgtgacgaca ggtacggtaa gcgaaattgt tgctaacact
      961 gaaccaaaga acagattcat cgcgcgctgg acctggttgt tcaacactgc ttttaatgca
     1021 cctaaacctt ccggcgacag aatcaacagt gccaccagga agccagtaaa ggcgacaggg
     1081 gcattcatgc tgtcgagcaa agtctccagc gggctggcgt tcattttggt gactgcaata
     1141 acggcaatca ggtggataat caaccagata gcatgccaca ggctgctatg ggcagacggt
     1201 ttaccgtggt gcgggtcgtc gtcatcactg tcatcttcgt gctcgtagac aaacaaactt
     1261 tgatgcgttt tggtctggat cagcaaaaat acgccataca ttgccgcaga aattaatgct
     1321 accagtaacg cctgaccggt tgaaaaattc gccgcaggca gagccattgg aaataccagt
     1381 acgattatcg ccagggggaa cagcgcaatt aaatactgct taataccaaa cagattcata
     1441 tattgggtgg caaacttacg accgcccaac aataatgaaa agccgaccag cccaccggta
     1501 acaatcataa tgatcgaata gagcgtatca cgcattagcg ttggcgcggc gtcgccggtt
     1561 gccattaaag ctgaaatcaa actgacttca agaataacca ctgaaaggct aagaataagc
     1621 gaaccgtaag gttctcccag gcgatgggct aatacgtccg catgacggac aacactaaag
     1681 gcgctactta aaataccaat aagcgcaaga agattgatgg caatgaccac tggtagtgtc
     1741 tggctgcttc cccacaggaa cagcactacc agcgccagaa ccgggaaaat aagcgaagtc
     1801 tccttgtggc gggtttttac cgcctcttga gcatttgaca ttatggttat ccctttgcag
     1861 atgaatttat cgaaaatgta aaaaataggt aggaaaaata acagaaagtg tctggatatc
     1921 ggtaacattt tacgaatttt taccccgttg tcgatttttt actcattggg gcataaaaat
     1981 aagtaatacg tttagacaat gtttgtttta gcgtttctta agaacagtct gacatgaaaa
     2041 ttcttatgtt ttggcaagag tagatattgt tgaccacact taatgttcaa ctttgtaaaa
     2101 ggagtcaacg atgccgtata aaacgaaaag cgacctgccg gaaagcgtaa agcacgttct
     2161 accgtctcat gcccaggata tctataaaga agcgttcaac agcgcatggg atcaatataa
     2221 agataaagaa gatcggcgtg atgacgccag tcgcgaagaa accgcgcata aagtggcctg
     2281 ggctgctgtg aagcatgaat atgccaaagg ggatgatgat aaatggcata aaaaatcgta
     2341 aaaccggtag cttagttaaa gctattcgtg cggtgttgcc ttgcaagtgg tccgtggatt
     2401 gcatattgtc ccgttagtgg tttcaaaatg agcagtaaaa atgtccggaa gacaccaaat
     2461 agttgtcgca gggaagtatg cagtggcgga ggtgtaaggt gataacgcgt gatttcttga
     2521 tgaatgccga ttgtaaaacg gcatttggtg ccattgaaga atcactctta tggtcagcag
     2581 aacaacgggc ggcttcgctg gcggcgacgc tggcttgtcg acctgatgag ggaccggtgt
     2641 ggatcttcgg ttatggatcg ttgatgtgga atccggcact ggagtttacc gagtcgtgca
     2701 ccggtacact ggttggatgg catcgtgcgt tttgcctgcg cctgaccgcc gggcgcggga
     2761 ctgcgctcca gccgggacgg atgcttgcac tgaaagaggg cggacgcacc acaggcgtcg
     2821 cctatcgact gccagaagag acgctggagc aggaactaac cctgttgtgg aagcgcgaga
     2881 tgattaccgg ctgttatctg ccaacctggt gtcagcttga tcttgatgat ggacgcacag
     2941 taaacgccat tgtgtttatt atggacccgc gacatccaga atatgaatct gacactcgcg
     3001 ctcaggtcat cgcgccgttg attgcggcgg cgagcggtcc gctgggaacc aacgcacaat
     3061 acctgttttc actggaacag gagcttatca aactgggaat gcaggacgat gggctgaatg
     3121 atttgctggt atcggtaaaa aaactgctgg cggagaattt tccggatggt gtgttacggc
     3181 cgggattcgc ctgagtaaac ttccctcatc gtggggcgtc agacgccccc ttaaacatta
     3241 aaatgtgagc actttatcgg ctgacagcgt ccattgcgcc agttccacaa gagtaccgat
     3301 ttccacccca tcaatcagag gaagtgtact aatcccgcgc ccgtcggtac aggttttgca
     3361 caatttcacc ggtacattct gagcggtaag gatctccagc atttgctgaa tgttgtagcc
     3421 ttcccctggt ttttgcccgc gcaacccggc tgtgaccgca tcagacatca ggaacagacg
     3481 cagatccaga ttgctctcct gctctcgtaa cgcaatggcc agccgcaagc tgttaaacaa
     3541 ggattcgctc ccgtaaggtg cgccattggc aacgatcacg attttttgca ttatttactc
     3601 ctgtattcag ggaattagac actcatcttc tatcttactg cttctgcagc gtctgaccaa
     3661 tcggtcacat tattaaggat tttcctgaaa gcgcgagaaa atacgacaaa agttgccagt
     3721 aatcgttatt ctttaaggct atggtttttc attttttacc ggaagttacc gacgttttga
     3781 gccgtttcgt tcctcgcatt attccgtttt atttactctt gctggcggca ggcggtacag
     3841 ctaacgcaca atctaccttc gagcaaaaag cggcaaatcc ctttgataat aacaatgatg
     3901 gtctgccgga tttaggcatg gcacctgaaa atcatgatgg ggaaaaacac tttgctgaaa
     3961 ttgtgaaaga tttcggcgaa accagtatga atgataacgg gctggatact ggcgagcagg
     4021 caaaagcttt cgcattggga aaagtccgcg acgcgcttag tcaacaggtt aatcagcacg
     4081 tagagtcgtg gctatcaccg tggggaaatg ccagtgttga cgtcaaagtg gataatgaag
     4141 gtcattttac cggcagtcgt ggaagctggt ttgtgccgtt acaagataat gatcgttatc
     4201 tcacctggag ccagcttggt cttactcagc aggatgatgg gctggtgagc aatgtgggcg
     4261 ttgggcaacg ctgggcgcgc ggcagctggc tggtgggtta taacactttt tatgacaact
     4321 tgctggatga aaatcttcag cgagcgggct ttggtgccga agcgtggggc gaatatttgc
     4381 gactatcggc aaacttttat cagccgtttg ctgcatggca tgaacagaca gccacgcagg
     4441 aacaacggat ggcgcgcggg tacgacctga cagcccggat gcgcatgccg ttctatcaac
     4501 acctcaatac cagtgtcagc gtagaacagt attttggtga tcgtgttgat ttgtttaact
     4561 ctggtacggg ttatcacaat cccgtcgcgt tgagtctggg attaaattac acccctgtgc
     4621 cattagtcac tgtgacggcc cagcataaac agggtgaaag tggcgaaaat caaaataacc
     4681 tcggactgaa tcttaattac cgctttggtg taccgctcaa aaaacaactt tctgcgggcg
     4741 aggttgccga aagtcagtcg ttacgtggta gtcgctatga taatccgcag cgaaataatc
     4801 taccgactct tgagtaccga cagcgaaaaa cgttaacggt gtttctggcg acaccgccgt
     4861 gggatctaaa acctggcgaa acagtgccgc tgaaattaca aatccgcagt cgttacggta
     4921 ttcggcaact gatttggcag ggcgatacgc agatattaag tttgacgccg ggcgcacaag
     4981 ccaacagcgc ggagggctgg acgctgatca tgcctgactg gcagaacggg gaaggggcga
     5041 gcaatcactg gcgattgtcg gtggtggtgg aagataacca ggggcagcgt gtctcctcca
     5101 atgagatcac gctaacgctt gtcgaaccgt tcgacgcatt gtcaaacgac gaactgcgct
     5161 gggaaccgta atcagaaaat gcgctcctga tgcacccata ccgctgcttc cacgcgagac
     5221 ttgagcttca ttttcttcag catgtgcttg acgtgcactt ttactgtgct ttcggtgatg
     5281 tccaggcggc gggcaatcat cttgttcggc aaaccctggg caatcagctt gagaatatcg
     5341 cgctcgcgtg gggttaactg gttaacatcg cgctcagtcg tggcacggtt agcgcgcaag
     5401 ctggcggcca gaacaggcgt taatgcttcg cttaatacca tttcgccagc agctgcctga
     5461 tgcaatgctt tcagcagatc ttccggttcc atatctttta acagatagcc atccgcgccg
     5521 cgtttcagtg cggtgaccac atcttcttca tggttagaga cgctgaatac cacaatgcgc
     5581 cctgagaggg acttttcgcg cagtttatcc agcgtttcca gaccgttcat gccgggcata
     5641 ttgagatcta acaggatcag atcgggatca agagattccg ccagttcaat accctgttcg
     5701 ccattactcg cttcgccaac cacggtgata tctggtgcca tactgataag ctgttttacg
     5761 ccagttcgca gcatcgggtg atcgtcaatc agcaggatag tagccggttc ctgattactc
     5821 atgggtatct ccttggacgt ctgtgaaagt tttttcggga ataaaggtga ccaccacttc
     5881 ggtgccacct gattcacgac ggcggacgcg gcaatcgcct cgtaaacttt gcgcacggtc
     5941 gcgcattatt atcatgccgt agtgattgct gcggatggca ttttcaggca cgccgcagcc
     6001 gttatcctgg acggtcagtt tgacctgatt atcgttttgc gccaccgtca ccacgacctc
     6061 actcgcttgc gaatgtttga gggcgttact taatgcctca cgggcaattt gcaacaagtg
     6121 gattgcctga tgcgaaggca ccagacgagg cggcaattga taatccagct tcaccgggaa
     6181 gccaaatttg gcgctgtact cttcgcaact cgcctccagc gccggacgta atccaggctc
     6241 ggtgagctgc aagcggaatg tggtgagcaa ttcacgcaac tgcgcccagg atgcattcag
     6301 ttcgttacgg atctgactta acagttcgcg gctgctttct ggcagcgcat cgccctgcat
     6361 ctgtaaacaa ctcacctgca tcttcatgca agagagagat tgggcaatag aatcatgcag
     6421 ttcgcgcgca atggtggcac gctcttccat cacgatcaac tgttgctgac gttcctgatg
     6481 ccgatccagc gccagcgtgg cggtgagttg ttcaaccagg gtatccacca gttgttgttg
     6541 atcatggcta agatgacgcc cctgcggcag ggtcgccagc aaaataccgt actgcgtatg
     6601 agagtcagcc agccgccact tcagggtcgt gccgcgatca ccaacgggta atacgccgcg
     6661 cgggcagagc tggcagcctt tatcatcaca agtcatatct ggctggcagg taaactcctg
     6721 atgattctct tcatcatcag tgtcatacac ccgcagttcg atatcacgta gcagggttaa
     6781 attctgtaag ccgttgagta caggtgacag gcgttcacac agcggggcgc gggaatgcaa
     6841 acggcggtta gcctgccata aaaaagagag gatctgattt ttatgctcca gcccggcggt
     6901 tttctcctga acccgctgct caagtacggc ataactttcg gccagttctg cagacatatt
     6961 gttcaacgca gttccaagca tcgccatttc gttgcgcccg ctgatgtttg cgcgttgggt
     7021 aaaatcgcga tggctgacgg cactcgccat tgccagcagt tgccgccacg gttgtagcag
     7081 tcgcgcccgc aaccagataa tagtgaacac cagtaaaagt gccataaata ccgccatcac
     7141 ccgatggacc agtaccactg tctcaatacg catttccgtg gtgcggtcaa aaccagatac
     7201 cagctgatca agcccggcaa caaactggct gacatccgct gataccgttt ctcggttttg
     7261 tgcccgcatc agcgcaggga tcagttcatt acgccagtaa tcttgtaaac cctgtaattg
     7321 cgccagttgt ccgtctcgtt ctgctgctcg agtcaactcg gcgctaaatg ccgtttgttc
     7381 catctcttta attaagggct tgtctttctc gcttaatggc actgctgcca acagacggta
     7441 actttgcatg cgcagcgatc ctgctttgtt gatcgcatgg gcgctgccct gaacgccttg
     7501 caccagccag ccagaaaccg ccatccctgc cagtccaata gcagtagaaa gcaacacaat
     7561 aagcgcaacc tgattaacca gggtgagcgg agagagacaa cgtttaagca tgtaaacctc
     7621 ttccttcagg ctttaaatga gcaataacct taatgaatgt gacgatacat tctggaatgg
     7681 cagtattctc ggctattggc tgaagtatac ccatacccgg aaagagttac tccttatttg
     7741 ccgtgtggtt agtcgcttta catcggtaag ggtagggatt ctacagcgcc gtgaaaaatc
     7801 tcataatttt tatgaaatca ctgtactcac tatgggtaat gataaatatc aatgatagat
     7861 aaagttatct tatcgtttga tttacatcaa attgccttta gctacagaca ctaaggtggc
     7921 agacatcgaa acgagtatca gaggtgtcta tgagtcactc atccgccccc gaaagggcta
     7981 ctggagctgt cattacagat tggcgaccgg aagatcctgc gttctggcaa caacgcggtc
     8041 aacgtattgc cagccgcaac ctgtggattt ccgttccctg tctgctgctg gcgttttgcg
     8101 tatggatgtt gttcagtgct gttgcggtga acttaccgaa agtcggtttt aattttacga
     8161 ccgatcagct atttatgttg actgcgctgc cttcggtttc tggcgcgtta ttacgtgttc
     8221 catactcctt tatggttcct atcttcggtg gtcgtcgctg gacggcgttc agcaccggta
     8281 ttctgattat tccttgtgtc tggctgggtt ttgccgtgca ggatacctcc acgccttata
     8341 gcgtcttcat catcatctct ctgctgtgcg gctttgctgg cgcgaacttc gcttccagta
     8401 tggcaaacat cagcttcttc tttccgaaac agaagcaggg gggcgcgctg ggtctgaatg
     8461 gtggtctggg caatatgggc gtcagcgtca tgcagttggt tgctccgctg gtggtatcac
     8521 tgtcgatttt cgcagtattt ggtagccagg gcgtcaaaca gccggatggg actgagctgt
     8581 atctggcgaa tgcgtcctgg atatgggtgc cgttccttgc catcttcacc attgcggcgt
     8641 ggtttggcat gaacgatctt gctacctcga aagcctccat caaggagcag ttgccggtac
     8701 tcaaacgggg tcatctgtgg attatgagcc tgctgtatct ggcaaccttc ggctccttta
     8761 tcggcttctc cgcgggcttt gcgatgctgt caaaaacgca gttcccggat gttcagattc
     8821 tgcaatacgc tttcttcggg ccgtttattg gtgcgctggc gcgttctgca ggtggtgcat
     8881 tatctgaccg tctgggcgga actcgtgtca cgctggtgaa ctttattctg atggcgattt
     8941 tcagcggcct gctgttcctg accttaccga ctgacgggca gggcggaagc ttcatggcgt
     9001 tcttcgcggt cttcctggcg ctgttcctga cagctgggct gggtagtggt tccactttcc
     9061 agatgatttc agtgatcttc cgtaaactga caatggatcg cgtgaaagca gaagggggtt
     9121 ctgacgaacg tgcgatgcgt gaagcggcaa ccgacacggc ggcggcgctg ggtttcatct
     9181 ctgcgattgg cgcgattggg ggcttcttta tcccgaaagc gtttggtagc tcgctggcat
     9241 taacgggttc gccagtcggc gcaatgaaag tatttttaat tttctatatc gcctgcgtag
     9301 tgattacctg ggcggtatat ggtcg
//
LOCUS       DAPGOF010000189         9313 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.190, whole genome shotgun sequence.
ACCESSION   DAPGOF010000189 DAPGOF010000000
VERSION     DAPGOF010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.190"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            117..1685
                     /gene="cydA"
                     /locus_tag="RHV13_004023"
     CDS             117..1685
                     /gene="cydA"
                     /locus_tag="RHV13_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HDV1243498.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            1701..2840
                     /gene="cydB"
                     /locus_tag="RHV13_004024"
     CDS             1701..2840
                     /gene="cydB"
                     /locus_tag="RHV13_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDV1243499.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            2855..2968
                     /gene="cydX"
                     /locus_tag="RHV13_004025"
     CDS             2855..2968
                     /gene="cydX"
                     /locus_tag="RHV13_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HDV1243500.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            2968..3261
                     /gene="ybgE"
                     /locus_tag="RHV13_004026"
     CDS             2968..3261
                     /gene="ybgE"
                     /locus_tag="RHV13_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HDV1243501.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMLWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            3411..3815
                     /gene="ybgC"
                     /locus_tag="RHV13_004027"
     CDS             3411..3815
                     /gene="ybgC"
                     /locus_tag="RHV13_004027"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HDV1243502.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            3812..4504
                     /gene="tolQ"
                     /locus_tag="RHV13_004028"
     CDS             3812..4504
                     /gene="tolQ"
                     /locus_tag="RHV13_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HDV1243503.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            4508..4936
                     /gene="tolR"
                     /locus_tag="RHV13_004029"
     CDS             4508..4936
                     /gene="tolR"
                     /locus_tag="RHV13_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HDV1243504.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            5001..6266
                     /gene="tolA"
                     /locus_tag="RHV13_004030"
     CDS             5001..6266
                     /gene="tolA"
                     /locus_tag="RHV13_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HDV1243505.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPTLCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            6399..7691
                     /gene="tolB"
                     /locus_tag="RHV13_004031"
     CDS             6399..7691
                     /gene="tolB"
                     /locus_tag="RHV13_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HDV1243506.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            7726..8247
                     /gene="pal"
                     /locus_tag="RHV13_004032"
     CDS             7726..8247
                     /gene="pal"
                     /locus_tag="RHV13_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HDV1243507.1"
                     /translation="MQLNKVLKGLMITLPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            8257..9048
                     /gene="cpoB"
                     /locus_tag="RHV13_004033"
     CDS             8257..9048
                     /gene="cpoB"
                     /locus_tag="RHV13_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HDV1243508.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSGDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            9213..9288
                     /locus_tag="RHV13_004034"
     tRNA            9213..9288
                     /locus_tag="RHV13_004034"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9246..9248,aa:Lys,seq:ttt)
BASE COUNT         2258 a         2244 c         2549 g         2262 t
ORIGIN      
        1 ttttgatgaa atggcaacga tagaggcgag caaataattt tgtattgggg catgcgtgtg
       61 accctttcta acggggttca ctctcggagt cttcatgcga tgagcaagga gtcatgatgt
      121 tagatatagt cgaactgtcg cgcttacagt ttgccttgac cgcgatgtac cacttccttt
      181 ttgtgccact gacgctcggt atggcgttcc tgctggccat tatggaaacg gtctacgtcc
      241 tttccggcaa acagatttat aaagatatga ccaagttctg gggcaagttg tttggtatca
      301 acttcgctct gggtgtggct accggtctga ccatggagtt ccagttcggg actaactggt
      361 cttactattc ccactatgta ggggatatct tcggtgcgcc gctggcaatc gaaggtctga
      421 tggccttctt cctcgaatcc acctttgtag gtctgttctt cttcggttgg gatcgtctgg
      481 gtaaagttca gcatatgtgt gtcacctggc tggtggcgct cggttcaaat ctgtccgcac
      541 tgtggattct ggttgcgaac ggctggatgc aaaacccaat cgcgtccgat ttcaactttg
      601 aaactatgcg tatggagatg gtgagcttct ccgagctggt gcttaacccg gttgctcagg
      661 tgaaattcgt tcacactgta gcgtctggtt atgtgactgg cgcgatgttc atcctcggta
      721 tcagcgcatg gtatatgctg aaaggtcgtg acttcgcctt cgctaaacgc tcctttgcta
      781 tcgctgccag cttcggtatg gctgccgttc tgtctgttat tgttctgggt gatgaatctg
      841 gttacgaaat gggcgacgtg cagaaaacca aactggctgc tattgaagcc gagtgggaaa
      901 cgcaacctgc gcctgctgcc tttactctgt tcggcattcc tgatcaggaa gaggagacga
      961 acaaatttgc gatccagatc ccttacgcgc tgggcatcat tgcaacgcgt tccgtggata
     1021 ctccagttat cggcctgaaa gaactgatgg tgcagcatga agaacgcatt cgtaacggga
     1081 tgaaggcgta ctctctgctc gaacaactgc gttctggttc taccgaccag gcggttcgtg
     1141 accagttcaa tagcatgaag aaagacctcg gttacggtct gctgctgaaa cgctatacgc
     1201 caaacgtggc tgatgcgact gaagctcaga ttcaacaggc aaccaaagac tccattccgc
     1261 gtgtagcgcc gctgtacttc gcgttccgta tcatggtggc gtgtggcttc ctgctgctgg
     1321 caatcatcgc gctctctttc tggagtgtca tccgcaaccg cattggcgag aaaaaatggc
     1381 tgctgcgcgc cgcgctgtac ggtattccgc tgccgtggat tgctgtagaa gcgggctggt
     1441 tcgttgccga atatggtcgc caaccgtggg ctatcggtga agtgctgcct accgccgtgg
     1501 cgaactcgtc gctgaccgcc ggcgacctca tcttctcgat ggtgctgatt tgcggcctgt
     1561 ataccctgtt cctggtggca gaattgttct taatgttcaa gtttgcacgc ctcggcccaa
     1621 gcagcctgaa aaccggtcgc tatcactttg agcagtcttc cacgactact cagccggcac
     1681 gctaagacag gagtcgtcaa atgatcgatt atgaagtatt gcgttttatc tggtggctgc
     1741 tggttggcgt tctgctgatt ggttttgcag tcactgacgg tttcgacatg ggggtgggca
     1801 tgctcacccg tttcctcggt cgtaacgaca ccgagcgtcg aattatgatt aactccatcg
     1861 cgccacactg ggacggtaac caggtatggc tgatcaccgc aggcggcgca ctgtttgctg
     1921 cctggccgat ggtctatgcc gctgcgttct ccggcttcta tgtggcgatg atcctcgtgc
     1981 tggcgtcttt gttcttccgt ccggtcggtt ttgactaccg ctccaagatt gaagaaaccc
     2041 gctggcgtaa catgtgggac tggggcatct tcattggtag cttcgttccg ccgttggtga
     2101 ttggtgtagc gttcggtaac ctgttgcagg gcgtaccgtt caacgttgat gaatatctgc
     2161 gtctgtacta caccggtaac ttcttccagc tgcttaaccc gttcggcctg ctggcaggcg
     2221 tggtgagcgt agggatgatc attactcagg gcgcaaccta tctgcaaatg cgtaccgtgg
     2281 gcgaactgca cctgcgtacc cgtgcaacgg ctcaggtggc ggcactggtg acactggtct
     2341 gtttcgcact ggctggcgta tgggtgatgt acggtatcga tggttatgtc gtgaaatcga
     2401 caatggacca ttacgcagcc tctaacccgc tgaataaaga agtggttcgt gaagctggcg
     2461 catggctggt taacttcaac aacacgccaa ttctgtgggc tattccggca ctgggtgtgg
     2521 ttctgccgct gctgaccatc ctgactgcac gtatggataa agccgcgtgg gcgtttgtgt
     2581 tctcctccct gacgctggcc tgcatcatcc tgactgccgg tatcgcaatg ttcccgtttg
     2641 tgatgccgtc cagcaccatg atgaacgcaa gtctgacaat gtgggatgca acttccagcc
     2701 agctgacgct taacgtcatg acctgggttg cggtggttct ggtaccgatc attctgctct
     2761 acaccgcctg gtgttactgg aaaatgttcg gtcgtatcac caaagaagat attgaacgta
     2821 acacccactc tctgtactaa gtaaggagct aaaaatgtgg tatttcgcat ggattctggg
     2881 aacgcttctt gcctgttcgt ttggggtaat caccgcgctg gcgcttgagc acgtcgaatc
     2941 aggcaaagcc ggtcaagaag acatctgatg agtaagatta tcgcgacctt gtatgcggta
     3001 atggacaagc gccccctgcg ggcgctttcc ttcgtgatgg cgcttctgtt agcaggatgt
     3061 atgctttggg acccatctcg ttttgccgcg aagaccagtg aactggaaat ctggcatggt
     3121 ttattgctga tgtgggccgt ctgtgctggt gtgattcacg gcgtgggctt tcgtccgcag
     3181 aaggttcttt ggcaagggat tttttgccca ttgcttgccg atattgttct cattgtcggg
     3241 ctgattttct tcttctttta aatcagaatt ctctttaaaa aatcatgggc cgcgccaggc
     3301 ccataaattt ttacgctccc ttaacttgcc ctcattccca aacctcaatc gcgcgcgtat
     3361 agtagcagcg tttaaaagct ctaacttttg ttgcattacc gggatgtaaa gtgaatacaa
     3421 cgctgtttcg atggccggtt cgcgtctact atgaagatac cgatgccggt ggtgtggtgt
     3481 accacgccag ttacgtcgct ttttatgaaa gagcacgcac agagatgctg cgtcatcatc
     3541 acttcagtca gcaggcgctg atggctgaac gcgttgcctt tgtggtacgt aaaatgacgg
     3601 tggaatatta cgcacctgcg cggctcgacg atatgctcga aatacagact gaaataacat
     3661 caatgcgtgg cacctctttg gttttcacgc aacgtattgt caacgccgag aatactttgc
     3721 tgaatgaagc agaggttctg gttgtttgcg ttgacccact caaaatgaag cctcgtgcgc
     3781 ttcccaagtc tattgtcgcg gagtttaagc agtgactgac atgaatatcc ttgatttgtt
     3841 cctgaaggct agccttctgg ttaaacttat catgttgatt ttgattggtt tttcaatcgc
     3901 atcctgggcc attattatcc agcggacccg tattcttaac gcagcggcgc gcgaagccga
     3961 agcgtttgag gataaattct ggtctggaat cgaactctct cgcctctatc aagagagcca
     4021 ggggaaacgg gataatctga ccggttcgga acaaatcttt tacagcgggt tcaaagaatt
     4081 tgtgcgcctg catcgtgcca atagccatgc gccggaagcc gtagtagaag gggcgtcgcg
     4141 tgcgatgcgt atttccatga accgtgaact tgaaaatctg gaaacgcaca ttccgttcct
     4201 cggtacggtt ggctccatca gcccgtatat tggtctgttt ggtacggtct gggggatcat
     4261 gcacgccttt atcgccctcg gggcggtaaa acaagcaaca ctgcaaatgg ttgcgccagg
     4321 catcgcagaa gcgttgattg ctacagcaat cggtctgttc gccgctattc cggcggtaat
     4381 ggcttataac cgtctcaacc aacgcgtaaa caaactggaa ctgaattacg acaactttat
     4441 ggaagagttt accgcgattc tgcaccgcca ggcgtttacc gttagcgaga gcaacaaggg
     4501 gtaagccatg gccagagcgc gtggacgagg tcgtcgcgat ctcaagtccg aaatcaacat
     4561 tgtaccgttg ttggacgtac tgctggtgct gttgctgatc tttatggcga cagcgcccat
     4621 catcacccag agcgtggagg tcgatctgcc agacgctact gaatcacagg cggtgagcag
     4681 taacgataat ccgccagtga ttgttgaagt gtctggtatt ggtcagtaca ccgtggtggt
     4741 tgagaaagat cgcctggagc gtttaccacc agagcaggtg gtggcggaag tgtccagccg
     4801 tttcaaggcc aacccgaaaa cggtctttct gatcggtggc gcaaaagatg tgccttacga
     4861 tgaaataatt aaagcactga acttgttaca tagtgcgggt gtgaaatcgg ttggtttaat
     4921 gacgcagcct atctaaacat ctgcgtttcc cttgcttgaa agagagcggg taacaggcga
     4981 acagtttttg gaaaccgaga gtgtcaaagg caaccgaaca aaacgacaag ctcaagcggg
     5041 cgataattat ttcagcagtg ctgcatgtca tcttatttgc ggcgctgatc tggagttcgt
     5101 tcgatgagaa tatagaagct tcagctggag gcggcggtgg ttcgtccatc gacgctgtca
     5161 tggttgattc aggtgcggta gttgagcagt acaaacgcat gcaaagccag gaatcaagcg
     5221 cgaagcgttc tgatgagcag cgcaagatga aggaacagca ggctgctgaa gaactgcgtg
     5281 agaaacaagc ggctgaacag gaacgcctga agcaacttga gaaagagcgg ttagctgctc
     5341 aggaacagaa aaagcaggct gaagaagccg caaaacaggc cgagttaaag cagaagcaag
     5401 cggaagaggc ggcagcgaaa gcggcggcag atgctaaagc gaaggccgaa gcggatgcta
     5461 aagctgcgga agaagcagcg aagaaagcgg ctgcagacgc gaagaaaaaa gcagaagcag
     5521 aagccgccaa agccgcagcc gaagcgcaga aaaaagccga ggcagccgct gcggcactga
     5581 agaagaaagc ggaagcggca gaagcagctg cagctgaagc aagaaagaaa gcggcaactg
     5641 aagctgctga aaaagccaag gcagaagctg agaagaaagc ggctgctgaa aaggctgcag
     5701 ctgataagaa agcggcagca gagaaagctg cagccgacaa aaaagcagca gaaaaagcgg
     5761 ctgctgaaaa ggcagcagct gataagaaag cagcggcaga aaaagccgcc gcagacaaaa
     5821 aagcggcagc tgcaaaagca gcagctgaaa aagccgctgc agcaaaagct gccgcagagg
     5881 cagatgatat tttcggtgag ctaagctctg gtaagaatgc accgaaaacg gggggagggg
     5941 cgaaagggaa caatgcttcg cctgccggga gtggtaatac taaaaacaat ggcgcatcag
     6001 gggccgatat caataactat gccgggcaga ttaaatctgc tatcgaaagt aagttctatg
     6061 acgcatcgtc ctatgcaggc aaaacctgta cgctgcgcat aaaactggca cccgatggca
     6121 tgttactgga tatcaaacct gaaggtggcg atcccacact ttgtcaggct gcgttggcag
     6181 cagctaaact tgcgaagatc ccgaaaccac caagccaggc agtatatgaa gtgttcaaaa
     6241 acgcgccatt ggacttcaaa ccgtaatcgc gatgttgact gttccgacgg tcaacatcag
     6301 gcaccggttg ccacggggtt ctggtagttt tgtgtatttt agtttgttaa cattctgcta
     6361 aattatcgtg ggccatcggt ccagataagg gagatatgat gaagcaggca ttacgagtag
     6421 catttggttt tctcatactg tgggcatcag ttctgcatgc tgaagtccgc attgtgatcg
     6481 acagcggtgt agattccggt cgtcctattg gtgttgttcc tttccagtgg gcggggcctg
     6541 gtgcggcacc tgaagatatt ggcggcatcg ttgctgctga cttgcgtaac agcggtaaat
     6601 ttaatccgtt agatcgcgct cgtctgccac agcagccggg tagtgcgcag gaagtacaac
     6661 cagctgcatg gtccgcactg ggcattgacg ctgtagttgt cggtcaggtc actccgaatc
     6721 cggatggttc ttacaatgtt gcttatcaac ttgttgacac tggcggcgca ccgggtactg
     6781 tacttgctca gaactcgtac aaagtgaaca agcagtggct gcgttatgct ggtcataccg
     6841 ccagtgatga agtgtttgaa aaactgaccg gcattaaagg tgcgttccgt acccgtattg
     6901 cctacgttgt tcagaccaac ggcggtcagt tcccgtatga attgcgcgta tctgactatg
     6961 acggttacaa ccagtttgtc gttcaccgtt caccgcagcc gctgatgtcc ccggcgtggt
     7021 caccagacgg ttctaaactg gcttatgtga ccttcgaaag cggtcgttcc gcgctggtta
     7081 ttcagacgct ggcaaatggc gctgtacgtc aggtggcttc attcccgcgt cacaacggtg
     7141 cgcctgcatt ctcgccagac ggcagcaaac tggcattcgc cttgtcgaaa accggtagcc
     7201 tgaacctgta cgtaatggat ttggcttctg gccagatccg ccaggtgact gatggtcgca
     7261 gtaacaatac cgaaccgacc tggttcccgg acagccagaa cctggcattt acttctgacc
     7321 aggccggtcg tccacaggtt tataaagtga atatcaacgg cggtgcgcca caacgtatta
     7381 cctgggaagg ttcgcagaac caggatgcgg atgtcagcag cgacggtaaa tttatggtaa
     7441 tggtcagctc caacggtggg cagcagcaca ttgccaaaca agatctggca acgggaggcg
     7501 tacaagttct gtcgtccacg ttcctggatg aaacgccaag tctggcacct aacggcacta
     7561 tggtaatcta cagctcttct caggggatgg gatccgtgct gaatttggtt tctacagatg
     7621 ggcgtttcaa agcgcgtctt ccggcaactg atggacaggt caaattccct gcctggtcgc
     7681 cgtatctgtg ataataatta attgaatagt aaaggaatca ttgaaatgca actgaacaaa
     7741 gtgctgaaag ggctgatgat tactctgcct gttatggcaa ttgcggcatg ttcttccaac
     7801 aagaacgcca gcaatgacgg cagcgaaggc atgctgggtg ccggcactgg tatggatgcg
     7861 aacggcggca acggcaacat gtcttccgaa gagcaggctc gtctgcaaat gcaacagctg
     7921 cagcagaaca acatcgttta cttcgatctg gacaagtacg atatccgttc tgacttcgct
     7981 caaatgctgg atgcacatgc aaacttcctg cgtagcaacc cgtcttacaa agtcaccgta
     8041 gaaggtcacg cggacgaacg tggtactccg gaatacaaca tctccctggg tgaacgtcgt
     8101 gcgaacgccg ttaagatgta cctgcagggt aaaggcgttt ctgcagacca gatctccatc
     8161 gtttcttacg gtaaagaaaa acctgcagta ctgggtcatg acgaagcggc atactccaaa
     8221 aaccgtcgtg cggtactggt ttactaagag aattgcatga gcagtaactt cagacatcaa
     8281 ctattgagtc tgtcgttact ggttggtata gcggccccct gggccgcttt tgctcaggca
     8341 ccaatcagta gtgtcggctc aggctcggtc gaagaccgcg tcactcaact tgagcgtatt
     8401 tctaatgctc acagccagct tttaacccaa ctccagcaac aactctctga taatcaatcc
     8461 gatattgatt ccctgcgtgg tcagattcag gaaaatcagt atcaactgaa tcaggtcgtg
     8521 gagcggcaaa agcagatcct gttgcagatc gacagcctca gcagcggtgg tgcagcggcg
     8581 caatcaacca gcggtgatca aagcggtgcg gcggcatcaa cgacgccgac agctgatgct
     8641 ggtactgcga atgctggcgc gccggtgaaa agcggtgatg caaacacgga ttacaatgca
     8701 gctattgcgc tggtgcagga taaatcccgc caggatgacg caatggtggc atttcagaat
     8761 ttcatcaaaa attaccctga ttcaacttac ctgccaaacg ccaattattg gctgggtcag
     8821 ttaaactaca acaagggtaa aaaagatgat gcggcgtact attttgcttc ggtagtgaaa
     8881 aactatccga agtcaccaaa ggctgcagat gcgatgttta aagtcggcgt catcatgcag
     8941 gacaaaggtg acaccgcaaa agcgaaagcc gtataccagc aggttatcag taaataccct
     9001 ggtactgatg gcgctaaaca ggcacaaaaa cgtctgaacg cgatgtaatg cataacacac
     9061 gaccagaagt cgcattattt ctggtcgtgt cgtgcgaatc ataagcagtt gagtgatcta
     9121 catcgaaatt tttgttgcgc tcaagtctga aatcagtaat atatgccgcc gttgccacgg
     9181 gatatcaaac aaaccgaaag caacgaaaaa gtgggtcgtt agctcagttg gtagagcagt
     9241 tgacttttaa tcaattggtc gcaggttcga atcctgcacg acccaccact aacctagtta
     9301 gttgtagtat cca
//
LOCUS       DAPGOF010000190         9085 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.191, whole genome shotgun sequence.
ACCESSION   DAPGOF010000190 DAPGOF010000000
VERSION     DAPGOF010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9085
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.191"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            48..410
                     /locus_tag="RHV13_004035"
     CDS             48..410
                     /locus_tag="RHV13_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243509.1"
                     /translation="MNEFEKIFNEMNLDRALLPILFRSNRSTVWKYLSGDSTAPASAM
                     SLIMLLQLIQKRNPDLLAEWLTLSDFTIPPEVYLDQPDYWKGWVYTQHKVNKNVLEYL
                     KKHYPDEDQKSMSKGREE"
     gene            complement(589..1047)
                     /locus_tag="RHV13_004036"
     CDS             complement(589..1047)
                     /locus_tag="RHV13_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDV1243510.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLIIRAVIA"
     gene            complement(1044..1961)
                     /locus_tag="RHV13_004037"
     CDS             complement(1044..1961)
                     /locus_tag="RHV13_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HDV1243511.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            2107..2784
                     /gene="fetA"
                     /locus_tag="RHV13_004038"
     CDS             2107..2784
                     /gene="fetA"
                     /locus_tag="RHV13_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HDV1243512.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKVEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            2771..3550
                     /gene="fetB"
                     /locus_tag="RHV13_004039"
     CDS             2771..3550
                     /gene="fetB"
                     /locus_tag="RHV13_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HDV1243513.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(3613..4467)
                     /gene="cnoX"
                     /locus_tag="RHV13_004040"
     CDS             complement(3613..4467)
                     /gene="cnoX"
                     /locus_tag="RHV13_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HDV1243514.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(4528..5337)
                     /gene="ybbO"
                     /locus_tag="RHV13_004041"
     CDS             complement(4528..5337)
                     /gene="ybbO"
                     /locus_tag="RHV13_004041"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HDV1243515.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(5327..5950)
                     /gene="tesA"
                     /locus_tag="RHV13_004042"
     CDS             complement(5327..5950)
                     /gene="tesA"
                     /locus_tag="RHV13_004042"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HDV1243516.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            5921..6607
                     /locus_tag="RHV13_004043"
     CDS             5921..6607
                     /locus_tag="RHV13_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV1243517.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            6604..9018
                     /locus_tag="RHV13_004044"
     CDS             6604..9018
                     /locus_tag="RHV13_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV1243518.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMSGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGSENQLDSYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLALSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVTPLKAF
                     LAEHQIVPESFYPVVRARLTAINGKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVTLGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCCGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT         2055 a         2344 c         2512 g         2174 t
ORIGIN      
        1 gataaatctc aatttttaag aggtttacgc caggcaggaa aatttaaatg aatgaatttg
       61 agaagatttt taatgaaatg aatctggacc gggcattgct accgatcctt tttcgttcga
      121 accggtcgac agtgtggaag tatttaagcg gcgatagtac ggctcctgcc tctgccatgt
      181 cgctgattat gctgttgcag ttgatccaga aacgaaaccc cgatctgtta gccgaatggt
      241 taacgctttc tgatttcacc attccgccag aagtttacct ggaccagcct gattactgga
      301 aagggtgggt gtatacccaa cacaaggtca ataaaaacgt cctggaatat ctaaaaaaac
      361 actatccgga tgaagaccaa aaaagtatga gtaaaggccg ggaagagtaa attgccaatg
      421 ccagttaaca tttaacatgg cggaggcggc ctacggtttt ggcgtgatat gacgcgcccg
      481 cgtcgcatca ggcgtcggtg cacgactgcc ggatgcggcg taaacgcctt atccggccta
      541 cgattcgggc gcagacttcc gggcatgata atacgcttca gcgtcgcatc aggcgatgac
      601 cgcacggatg atcagcgtta tcccttctat cgcaatgact tcaacatgcg tacctgcgcc
      661 gagatcctcg ctggcgctga caggccatga actgtcaccg acgcgcatat gaccgcgccc
      721 gttgaccagc ggagattcca gcacaaaacg tcggccaatc agctgctgcc cgcgctggtt
      781 taaatgacta tcgctgtgct tttgttcgcg cacccgccgc gacaaccatt tccaccacag
      841 ccaggcggcg agcagcgtca ggatggcaaa catcacccct tgccactccc aacccagcgg
      901 caccagccag actaccaggc cggtaatcac cgctgccacg ccgctccaca acaaataacc
      961 atttccgccc agcatctcgg ctgccagcag caaaccgccg agactgagcc agaaaatatg
     1021 tggatgaacg accattaact ccatcatggc tgagtccgtt tgttggcact gtctttcacc
     1081 agctcggcaa tcccggcaat cgaccccatc aggctgctgg cctctaatgg catcatcact
     1141 actttgctgt tactggagga accgatctgc tgtaacgctt cggtgtattt ctgcgctacg
     1201 aagtagttca ccgcctgaat atcaccggag gcgatagctt cagacaccat tttggtggcg
     1261 cgggcttctg cttcggcgga acgttcacgc gcttcagcct gtaaaaacgc cgactgacgt
     1321 tcgccttccg ctttcaggat ttgcgactgt ttttcacctt ctgctttgag gatttccgcc
     1381 tgacggatcc cttccgcttc aagaatgtaa gcgcgtttgg tacgttccgc tttcatctgc
     1441 gcgttcattg aagagataag ctctgccggt gggcgcacgt cgcgaatttc aatacgggtg
     1501 actttaatcc cccacgggtt ggtggcttca tcgacaatac gcagcaggcg tgagttgatg
     1561 ctgtcgcgct gagagagcat ttcgtcaagt tccattgaac ccaacacggt acggatgtta
     1621 gtcatggtca ggttgatgat cgccagctcc agattgctga cttcataggc agcgcgcggc
     1681 gcgtcaatca cctgaataaa acagactgcg tcgatggtaa cgttggcgtt atctttcgag
     1741 ataacttcct gggaagggat atcgagcact tgctccatca tattgatctt gcgaccaatg
     1801 cgatccataa acggcaccac cagactgagc cccggctgta acgttttggt atagcgacca
     1861 aaacgttcta ccgtccattg atagccctgc ggcacgattt tgacgcccgc gccgacaatg
     1921 accagcgcga caaaaatgag aatcgggata aagataagca tcggaaaaac ctcctgttgt
     1981 accgtccata atcagcaaaa ttgctgcttg attaaacaaa ttatacctga ttactgaaag
     2041 agagttcccc cttattcctg cgaaggataa actgttttta gtaaaaatca gaaaaaggga
     2101 acagcgatgc aggaaaatag tcctttgctt cagctacaaa acgtaggata tctggcgggt
     2161 gatacgaaga ttcttaataa catcaatttt tcgctgcgtg ctggcgaatt taagttaatt
     2221 accggtcctt ctggttgtgg caaaagtacg ctgctaaaaa tagttgcttc attgatcagc
     2281 ccaaccagcg gaacgttact gtttgaaggt gaggatgtca gcacactaaa gccagaaatc
     2341 taccgccagc aagtctctta ctgcgcccag acaccgacgc tgtttggcga tacggtatac
     2401 gataatctga tctttccctg gcagatccgt aaccggcagc ctgacccagc catttttctc
     2461 gattttctcg aacgcttcgc cttgccggac agcattttga cgaaaaatat cgccgagcta
     2521 tctggtggtg aaaaacaacg catctcactg attcgtaacc tgcaatttat gccgaaggtt
     2581 ttattgctgg atgaaatcac cagtgcgctg gatgaaagta ataaacataa cgttaatgag
     2641 atgatccatc gttatgtgcg cgagcaaaat attgccgtgc tgtgggtgac acacgataaa
     2701 gtcgaaatta atcatgcgga taaagtgatt acattgcaac cgcatgccgg agaaatgcag
     2761 gaagcacgct atgaactcgc ataatattac taacgaatca ttagcactgg cattaatgct
     2821 ggtagtggtg gcaatcttaa ttagccataa agaaaaactg gcgctggaga aagatattct
     2881 ctggagcgtc gggcgcgcga taattcagct gattattgtt ggctatgtgc tgaagtatat
     2941 tttcagcgtg gatgatgcca gcctgacatt attgatggtg ttatttatct gctttaatgc
     3001 ggcgtggaac gcgcaaaaac gcagtaaata tattgctaaa gcttttatct catcgtttat
     3061 tgccattacg gtcggggcgg gaattaccct ggcggtgctg attctctcag ggtcgattga
     3121 atttatcccg atgcaggtga tccctatcgc cgggatgatt gccggtaacg ccatggtagc
     3181 ggtggggttg tgttacaaca atttagggca acgggtcatt agcgaacagc aacagattca
     3241 ggagaaactg agtcttggcg cgacgccgaa gcaggcttca gcgatattga ttcgcgacag
     3301 tattcgcgcg gctttaattc cgacggtcga ttcagcaaaa acggttggct tagtgagttt
     3361 accaggaatg atgtccgggc tgatatttgc cgggattgat ccggtgaagg cgattaaata
     3421 tcagattatg gtgaccttta tgctgctctc aaccgccagc ttgtcgacca ttattgcctg
     3481 ctatttaacc tatcgtaagt tttataattc gcgccaccag ttggtggtga cgcaattgaa
     3541 gaagaaatga tgcgatgccg gatgcgcagc atcgcatccg gcgttgtggt tcatgtgccg
     3601 atcaacggaa tatcaataca acaatgcata cagctggcgg cgatacttcg acgccagtgc
     3661 atcacccgta cccagcgcag cgaggatctc ctggaacgtt ttacgcgtct gaccgtctgc
     3721 ggcggtgaga tctttacgca gatgcccgaa cagcaactcc agcgcctctt cattgcgccc
     3781 aacctgatgc agttgcagcg ccagttgcgt cgccagtgcg gcatcttctg gattctctgc
     3841 cacctgctgt tgcagctgtt gaatttccgg cgtatcagcc gcctgcttca gcagttcgat
     3901 ttgcgccacc agcccctggt agcgggtgtc ctgatcctgc aatggaatgg ttttcagcac
     3961 cgcttctgca tcttcagaac ggttcagcgc aatcagcgtt tctgccaaca gcaggccgat
     4021 ttccccgttc tgattcgaca actgccaggc gtctttcagc aatggcaggg cgtcggtgta
     4081 attgccttcc tgcatcagtt gcatcgcctg ctgcgctttc agctcttctt cgcgcggcag
     4141 cactttatcc agcagggcgc ggatcgcctc ttccggttgc ggcccctgga agccgtctac
     4201 cggttgcccg ttctggaaca gatacacggt cggaatcgcg cgcagaccaa actgggcggc
     4261 aatcatctgc tccgcgtcgc agtccagctt cgccagaata aattgcccgt tgtactgcgc
     4321 cgcgaggctt tccagaattg gggttaactg caaacagtgc tggctacgtt cagaccaaaa
     4381 atagaacagc accggagtgg tcatcgactg ttcaagaacc tgttgcaggt tagattcgtt
     4441 aatgttgaca atattttcta cggacatgga gtcgctctct gttgtcgata ttttctttga
     4501 catgggggct taagcgcgcg cttcaactca cccctgcaat attttgtcca tcacgcgccc
     4561 cggcagcagg cgcttaagca ccattactgc ccaggtcacc agcgttaccg gatagcgcat
     4621 cttcggcttc tcgctaataa aagcatggcg tactttgtcc accaccgctt ccggtcccaa
     4681 cgtaaagcgg gcggcgatgc cgggattttc gactggttta tcactttgcg tctggttgac
     4741 gttgtcggtg aagcgagtac gaatgggacc gggttcgatc aggctgactt taattccgct
     4801 gtggcgtagc tccatgcgca gcgcgtctga ccacgcctcc agcgcatatt tactggccgc
     4861 gtaagcgcca cgacccggcg tggagattaa tcccatcacc gatgatgtca tcacaatacg
     4921 cccttcaccg tgcggtaaca tcgcgggtaa caggcgcatg gtgagctggt gtgcgccgaa
     4981 aaagttggcg gaaaactgct gttccatctg cgcacggctg atggtggaaa gggggccata
     5041 catgccgaat ccggcattgt taaagatccc atacagacaa ttatcggtca gggcgatcac
     5101 ctcgtcggct gcgcgatcaa cactttctgg tgaatccaga tcgatcaaca cgccggtaaa
     5161 tcccatgctg ttcatgcgct caacatcatc cggtttccgg caacctgcca gcacatgaaa
     5221 accctggcgt tttaattcga gcgcgctttc caggccaatt ccactggaac atccggtaat
     5281 taagaccgat ttttgcataa ctttacctgt caggatctcc gttgctttat gagtcatgat
     5341 ttactaaagg ctgcaactgc ttcgccatcc agtcggcaat aaacggctgg gcgtcgcggt
     5401 tgggatgaat accgtcatcc tgcatccatt gtggcttgag gtagacctct tccataaaaa
     5461 agggcagcag cggaacatca aactctttgg cgagtttggg gtaaatggcg ctaaaggctt
     5521 cattataacg gcgaccatag tttgcaggca gacgtatttg cattaacaat ggttcagcgt
     5581 tggcggcttt gacatcctgc aaaatctggc gcagcgtttg ctcggtttgc tgtggctgaa
     5641 aaccacgcaa accgtcattg ccgcccagtt caaccagcac ccaacgcggc tgatgctgtt
     5701 tcagcagagc cggaaggcgc gccagccctt gttgcgaggt gtcgccgctg atgctggcat
     5761 taactaccga cgttttactc tgccacttat cattcaacaa ggcaggccag gccgcgctgg
     5821 cagacattcg atacccggcg ctcaggctat cacccagaat caataacgtg tccgctgcgg
     5881 cggcacggaa ggttaacagg accaggaaca ggaagggcaa atgccagcgg aaaacattgt
     5941 tgaagttcat catcttaaga agtccgtcgg tcagggggag catgaactct ccatcctcac
     6001 cggagttgag ctggttgtca aacgtggcga gaccatcgca ctggtgggcg agtcgggatc
     6061 gggtaagtca accttgctgg cgatcctcgc cgggcttgat gacggcagca gtggcgaagt
     6121 gagtctggtg ggacaaccgc tacataatat ggacgaagaa gcgcgggcaa agttgcgcgc
     6181 gaagcacgtc ggctttgttt ttcagtcatt tatgttaatt cctaccctta acgcgctgga
     6241 aaacgtcgag cttccggctc tgctgcgcgg tgagagtagc gcggaaagtc gtaacggagc
     6301 gaaagcgttg ctcgaacagt tagggctggg taaacgtctg gatcatcttc cggcacagct
     6361 ttccggcggt gaacagcaac gagtggcgct ggcacgagcc tttaacggtc gacctgatgt
     6421 gctgtttgcc gacgaaccca ccggcaacct tgaccgccag acgggtgata aaattgccga
     6481 cctgctgttt tccctcaacc gtgagcatgg caccacgttg attatggtga cccacgacct
     6541 gcaactggcg gcacgctgcg accgctgctt acggctggtg aacgggcagt tgcaggagga
     6601 agcatgattg cacgttggtt ctggcgcgaa tggcgctcgc cgtcgttatt aattgtctgg
     6661 ctggcgctaa gcctggcggt ggcctgcgtg ctggcgctgg gcaatatcag cgatcgcatg
     6721 gagaagggct taagccagca aagccgggag tttatgtcgg gcgatcgggc gttgcgcagt
     6781 tcgcgcgaag tgccgcaagc gtggctggag gaagcgcaaa agcgcggcct gaaagtcggc
     6841 aagcagctga ctttcgccac catgacattt gcaggcgaca caccgcagct ggcgaacgtc
     6901 aaagcggtgg atgatatcta cccgatgtat ggcgatctgc aaaccaatcc ccctggcctg
     6961 aaaccgcagg cgggcagcgt attgctggcc ccacgcctga tggcactgct taacctgaaa
     7021 acgggcgaca ccattgacgt gggcgatgcc accttgcgga ttgccggaga agtgattcag
     7081 gaaccggatt ccggttttaa ccccttccag atggctccgc gtctgatgat gaatctggcg
     7141 gatgtcgata aaaccggagc cgtgcagccg gggagtcggg tcacctggcg ctataaattc
     7201 ggcggcagcg agaaccagct cgacagctat gagaaatggt tgttacctca gcttaaaccc
     7261 gaacaacgct ggtacggtct ggaacaggac gaaggcgcgc tggggcgatc gatggaacgc
     7321 tcgcaacagt tcctgctgct ttcggcgctt ctgaccttgc tgctggcagt ggcagcagtg
     7381 gcggtagcga tgaatcatta ctgtcgcagt cgctacgatc tggtggcgat cctcaaaacg
     7441 ctgggggcag ggcgagcgca actgcgtaag ctaatcgtcg gtcagtggtt gatggtgctg
     7501 gcgctttcag ccgttaccgg tggggcgata ggcctgttgt tcgaaaacgt gttgatggtg
     7561 ctgctcaagc ccgttctgcc tgctgcacta ccgcctgcca gtctctggcc gtggctgtgg
     7621 gcgcttggca ccatgacagt catctcgctg ctggtggggc tacgaccgta ccgcttgttg
     7681 ctggcaacgc agcctttacg cgtattacgt aatgatgtgg tagcgaacgt ctggccgctg
     7741 aagttttatc tgccgattgt cagtgtggtg gttgtgctgc tgcttgccgg attaatgggc
     7801 ggcagtatgc tgctctgggc ggtgctggcg ggcgcggtag tactggcttt gctgtgcggt
     7861 gtgctgggct ggatgctgct gaatgtactt cgccgcatga cgctgaaatc gctgcctctg
     7921 cgcctggcgg ttagccgcct gttacgtcag ccgtggtcaa cgttaagcca gctttcagct
     7981 ttttcgctct cctttatgct gctggcgctg ctgctggtgt tgcgtggcga tctgctagac
     8041 cgctggcaac aacagctacc gccagaaagc ccgaactact ttttaatcaa catcgccaca
     8101 gaacaggtta cgccgctaaa agcgttcctc gcggaacatc agatagtccc ggaatcgttt
     8161 tatccggtgg tgcgagcgcg gctgacggcg attaacggca agccgacaga aggcaatgaa
     8221 gatgaggcgc tcaaccgcga actcaatctt acctggcaaa atacgcggcc cgatcataat
     8281 ccgattgtcg ccggtaactg gccgccaaaa gctgatgaag tgtcgatgga agaggggctg
     8341 gcgaaacgct taaacgttac cctcggagat accgtgactt ttatgggcga tacccaggag
     8401 ttccgcgcta aagtgaccag cctgcgcaaa gtggactggg aaagtttgcg gcctaatttc
     8461 tattttattt tcccggaagg ggcattagac gggcaaccgc agagctggct taccagtttc
     8521 cgctgggaga atggcaacgg catgttgacg caactcaacc gccagttccc gaccattagc
     8581 ctgttagaca ttggcgcgat tttaaaacag gtcggtcagg tgctggagca ggtaagtcgg
     8641 gcgctggagg tgatggtggt gctggtcacc gcctgcggta tgttgctgtt gctggcacag
     8701 gtgcaggtgg gaatgcgtca gcgtcatcag gagctggtgg tgtggcgcac actcggtgcg
     8761 gggaaaaaac tgctgcgcac gacattgtgg tgcgagttcg ccatgcttgg gtttgtttct
     8821 ggcctggtgg ccgcaattgg tgcggaaacg gcgctggcag tgttgcaggc gaaagtgttt
     8881 gatttcccgt gggagccaga ctggcgattg tggattgttc tgccgtgctg cggtgcgctg
     8941 ctgctgtcgc ttttcggcgg ctggctgggc gcgagactgg ttaagggtaa ggcgctgttc
     9001 aggcagtttg cggggtgatg agagtaaata aggggcgttc tggttgaatg gaacgccttg
     9061 tttaaattaa ttctacaata tgttt
//
LOCUS       DAPGOF010000191         9074 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.192, whole genome shotgun sequence.
ACCESSION   DAPGOF010000191 DAPGOF010000000
VERSION     DAPGOF010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.192"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            406..699
                     /locus_tag="RHV13_004045"
     CDS             406..699
                     /locus_tag="RHV13_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008925195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV1243519.1"
                     /translation="MNNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSR
                     LEANPASASIERLFKVFSILGVKISFSSTTASSEGKQTEEMLKSNSPARQEKW"
     gene            704..2029
                     /locus_tag="RHV13_004046"
     CDS             704..2029
                     /locus_tag="RHV13_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HDV1243520.1"
                     /translation="MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPL
                     SLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRLAMRYKADSLEPFDLLTELGKDC
                     VGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
                     QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYG
                     LPVARTQIAHFEDQKALVVERFDRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGG
                     PGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFSISIGPQGRYHLTPL
                     YDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQ
                     TEAMIEEIIARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK"
     gene            2090..2296
                     /locus_tag="RHV13_004047"
     CDS             2090..2296
                     /locus_tag="RHV13_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243521.1"
                     /translation="MTEEQKRIERAIELACRYGGTDEMHHLQWVVDQMVRELAGERYA
                     QIVADATSGEDGPDTYKWSVGIAP"
     gene            2397..2807
                     /locus_tag="RHV13_004048"
     CDS             2397..2807
                     /locus_tag="RHV13_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243522.1"
                     /translation="MAILAIIAVIALGGYACGFIKARELSALRKEMKLYVDRMEAAEL
                     DLHAIRSVAEHRLHGAGKQFADLYSRIDGATVTLTPEDQLLLATMPFNIQDVEDIVNE
                     RGPKTISGRPFWEVEPGPHDYEYGPQYPYFPDQR"
     gene            2820..3635
                     /locus_tag="RHV13_004049"
     CDS             2820..3635
                     /locus_tag="RHV13_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDV1243523.1"
                     /translation="MREPRPRHTLQVIRVPSLEVQDLGLTSFDSWLDENGYDKTNARN
                     NRTIWAREGGWHLKRCRNLETGTDDFWFIAFDGKGGKIYPLKTQRDYRAAYRKLEAEG
                     YAPAVIEQMTTGAAYNLAYPRSTLKQAETATSEPKRKPDVDIQGEHCERVVTQRSGVA
                     QGKFKALLIENFAGRCAVTGWVNGGVLDAAHIEHGTRYNPSNGILMTPTMHALFDADL
                     MGIDPATLTVHFKPGIELGELFEGRKITPLVYDLDLERLAVRWAGYQGLAHEQ"
     gene            3888..4313
                     /locus_tag="RHV13_004050"
     CDS             3888..4313
                     /locus_tag="RHV13_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243524.1"
                     /translation="MYKTALVMIIGILGCSKAFAATELQLQQKRVMQFCANASLPLLV
                     AGTTYANTSDNGRPEKERLAILKNSVASSTAYKMASPGVQIAMMSVVEDIADPKELAL
                     HQKEVRRLGASYLSDSGVSWASKTVSPFTAWCNFNRLES"
     gene            4862..5170
                     /locus_tag="RHV13_004051"
     CDS             4862..5170
                     /locus_tag="RHV13_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243525.1"
                     /translation="MRKFKYIICHQCEGHGTMENPAFENGFTQSEMAEWEPEMREKYF
                     AGAFDVRCDVCAGDGKLSVPNVAAMSFSERRVLAARRRDERLQAADERLSRQERAMGY
                     "
     gene            5186..5407
                     /locus_tag="RHV13_004052"
                     /pseudo
     CDS             5186..5407
                     /locus_tag="RHV13_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784386.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            complement(5441..8413)
                     /locus_tag="RHV13_004053"
     CDS             complement(5441..8413)
                     /locus_tag="RHV13_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HDV1243526.1"
                     /translation="MPRRSILSATERESLLALPDAKDELIRHYTFNETDLSVIRQRRG
                     AANRLGFAVQLCYLRFPGTFLGVDEPPFPPLLRMVAAQLKMPVESWSEYGQREQTRRE
                     HLVELQTVFGFKPFTMSHYRQAVHTLTELALQTDKGIVLASALVENLRRQSIILPAMN
                     AIERASAEAITRANRRIYAALTDSLLSPHRQRLDELLKRKDGSKVTWLAWLRQSPAKP
                     NSRHMLEHIERLKSWQALDLPAGIERQVHQNRLLKIAREGGQMTPADLAKFEVQRRYA
                     TLVALAIEGMATVTDEIIDLHDRIIGKLFNAAKNKHQQQFQASGKAINDKVRMYGRIG
                     QALIEAKQSGSDPFAAIEAVMPWDTFAASVTEAQTLARPADFDFLHHIGESYATLRRY
                     APQFLGVLKLRAAPAAKGVLDAIDMLRGMNSDSARKVPADAPTAFIKPRWAKLVLTDD
                     GIDRRYYELCALSELKNALRSGDVWVQGSRQFKDFDEYLVPVEKFATLKLASELPLAV
                     ATDCDQYLHDRLELLEAQLATVNRMAAANDLPDAIITTASGLKITPLDAAVPDAAQAM
                     IDQTAMLLPHLKITELLMEVDEWTGFTRHFTHLKTSDTAKDKTLLLTTILADAINLGL
                     TKMAESCPGTTYAKLSWLQAWHIRDETYSTALAELVNAQFRQPFAGNWGDGTTSSSDG
                     QNFRTGSKAESTGHINPKYGSSPGRTFYTHISDQYAPFSAKVVNVGIRDSTYVLDGLL
                     YHESDLRIEEHYTDTAGFTDHVFGLMHLLGFRFAPRIRDLGETKLFIPKGDAAYDALK
                     PMISSDRLNIKQIRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELG
                     RIERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFYRLGEIRDRSFEQQRYRA
                     SGLNLVTAAIVLWNTVYLERATSALRGNGTALDDTLLQYLSPLGWEHINLTGDYLWRS
                     SAKVGAGKFRPLRPLPPA"
     gene            complement(8416..8973)
                     /locus_tag="RHV13_004054"
     CDS             complement(8416..8973)
                     /locus_tag="RHV13_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDV1243527.1"
                     /translation="MQGQRIGYVRVSSFDQNPERQLEGVQVARVFTDKASGKDTQRPE
                     LERLLAFVREGDTVVVHSMDRLARNLDDLRRIVQGLTQRGVRMEFVKEGLKFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIVLAKQRGAYRGRKKSLNSEQIAELKRRVAA
                     GDQKTLVARDFGISRETLYQYLRED"
BASE COUNT         2190 a         2305 c         2521 g         2058 t
ORIGIN      
        1 agtaacattc accggagcac ctccggaacc ccatagccca ccagctgcta aaaatgtctg
       61 cctttgcttt taatactctc tctgcctcct ccccgaggca gagagtagct cgtagttatt
      121 tatacagctc acaacagggc cataagatac tccgcttctc gtttaccgat gatcctgatc
      181 tgtcagctct cttaccggat gctgtacgag cgtaacggct tattattgat agttctctca
      241 tatcaaacct cataaagatt tatgctgcca cttagtaaca gcctaaaggt tgtactctct
      301 atttaacagc ttttaggctg ttaatttgat attacagctt ttagggtgta tgtttagaaa
      361 acagcgtaca aggtgtatca gaggaactac tgaggccagc ttatgatcaa taatgattac
      421 ccgttaaaca cgctcaacca gttgcgtccc ttgctcattg gttttcgcaa ggcgaatggc
      481 ctgacgcaaa aagacctgtc cgaaaggtta ggggtcactc aacagacata ctcacgtctg
      541 gaagccaacc ctgccagtgc gagcattgaa cggctattca aggtgttttc tatcctgggc
      601 gtaaaaatca gcttctcctc gacaaccgcc tcttcagagg ggaagcagac ggaagaaatg
      661 cttaaatcaa actcacctgc acgacaggag aaatggtaac gatatgagcc ggaaacagca
      721 gcgtctggca atctggatga acggaatcaa agtcggatac tgggaaaaaa gtaaaggggt
      781 agacagctta caataccttc ctgactggag tgcagacgaa cagggaaggc cgctgtcatt
      841 gtcccttcct tttactcccg ggaaccaggt ctggcgtgga aatgtggtac gtgactattt
      901 tgataattta ctcccggaca gcgaaggcat acgcagacgt ctggcaatgc gttacaaggc
      961 cgatagcctg gagccttttg atcttctgac ggagctggga aaagactgcg tgggcgcgat
     1021 acagctgctt catgacggag atgaacccac agatttatat tccgtgaagt atcacccgct
     1081 tactgaatca gaaattgcgg caactttgcg taataccacg gagacattgc taccgggcag
     1141 gccagaagat aacgacgact tacgtttatc tattgccggt gctcaggaga aaacagccct
     1201 actgtggcat gaagatcggt ggtgtatgcc ggagggtaat acaccaacga cgcatatatt
     1261 caagctaccg ctcggacttg tggggaacat gaaagccgac atgcgctcgt cggttgaaaa
     1321 tgaatggctc tgctcggtac ttctcgaaca gtacgggctc cccgtggcaa gaacgcagat
     1381 tgctcacttt gaagatcaga aagcgctggt ggtagaacgt tttgaccgaa aatggtcagg
     1441 aaacggacaa tggatcattc gtttacccca ggaggatatg tgtcaggccc tgggcatttc
     1501 gccattacga aaatatcagg cggacggcgg gccaggtata tcggaaatta tggaagttct
     1561 gagtaattca gaccgtgctg agcgtgacaa agcgcagttt tttatgacgc agatagtttt
     1621 ctggatgatg gccgcaacag atggccacgc taaaaatttc agtatttcta ttgggccaca
     1681 gggacgttat caccttacac cactctatga tgtgttgtcg gcatggccgg tgatcggtca
     1741 tgggaacaat cagatatcct ggcagaagtg caaactggcg atggccgtac gtggcagtag
     1801 caactattac cagatatatc gaatacaacg acgccactgg atcaggcatg gtgagataac
     1861 cggcctgagt aaacagcaga cagaggcgat gatagaggaa atcatcgcca gaaccccggg
     1921 tgtcattgaa cgtgttagcg ggcttcttcc ggaccagttc ccccagcagc ttgcggaaag
     1981 tatttttgat ggaatgaggc agcaatgcag gcgcctggct gagaaatgac agccgcaatt
     2041 tatagtgtgc atgttttcag aatagctgat gtaagcgtga ggattcagta tgactgaaga
     2101 acaaaaaaga atagagcggg ctattgagtt ggcttgtcgt tacggcggaa ccgatgaaat
     2161 gcatcatctg caatgggtcg tggatcagat ggtaagagaa cttgctggag aacgttatgc
     2221 acagattgta gcagatgcca ccagcggaga agatgggcct gatacttaca aatggagtgt
     2281 tgggatcgcg ccttagcatc tgtcagtctg gggatgaaaa cgtacttaac atccagcgaa
     2341 ttcccagact tcacattaag actagagtaa cggccgcaat cacgtgagag aaaaaaatgg
     2401 caattttagc aatcattgca gttatcgccc tgggtgggta cgcctgcggg tttattaaag
     2461 cccgtgagct gagcgctttg cgtaaagaaa tgaaactata cgtggatcgc atggaagcgg
     2521 cggagctgga cttacacgca atcagaagtg tagcggagca ccggttgcac ggtgcaggta
     2581 aacagtttgc cgacctttac agccgtatcg acggtgcaac tgtgacgctg acacccgaag
     2641 atcagctgtt actggcaacg atgccattta atattcagga tgtagaagac atcgtaaacg
     2701 agcgcggtcc aaagacaatt agcggccgtc cattctggga agttgagcca ggcccgcatg
     2761 actatgaata tggcccgcaa tatccgtatt tcccagacca gcgctaggaa gagatcaaga
     2821 tgagagagcc aagaccccgc cataccttgc aggtcattcg agtgccttct ctggaggttc
     2881 aggatctggg gctgaccagc tttgatagct ggcttgacga gaacggttat gacaaaacga
     2941 atgccagaaa taaccgtacc atctgggcga gagaaggcgg atggcatctg aaacgttgca
     3001 gaaatctgga aactggcacg gacgatttct ggtttatcgc tttcgatggt aaaggcggaa
     3061 agatataccc gctgaaaacc caaagagact accgggctgc atatcgtaag ctggaagcag
     3121 aaggttatgc acctgcggtc atcgagcaaa tgaccacagg cgcagcatat aacctggcct
     3181 atccacgttc caccctaaaa caagctgaga cggccaccag cgaaccaaag cggaaacctg
     3241 acgtagatat ccagggtgag cactgcgagc gcgtagtgac gcaacgtagt ggcgtggcac
     3301 agggtaaatt taaagctctg ctcatcgaga atttcgcggg ccgttgtgcg gtaaccggtt
     3361 gggttaatgg cggtgtcctg gatgcggcac acattgaaca tggaacccgg tacaatccgt
     3421 ctaatggcat cctgatgact ccaacaatgc atgctctgtt tgatgccgat cttatgggca
     3481 tagatccggc caccctgacc gttcatttta agccaggcat tgagctgggc gagctgtttg
     3541 aaggccgtaa aatcacaccg ctggtgtacg atctggactt agagcggctg gccgtacgct
     3601 gggcaggata ccagggcctg gctcatgaac aatgatgggt tacagccggg atgtgtgaaa
     3661 acccgtcagt gaaacctgca tgcgccatac ctttaaattt aaactcaaga gccaggctca
     3721 agataaaacc gaaatgaaaa ggtaaaatcc ggtgcaccag atctcttagg tttaatacct
     3781 gagccggtat actggcaggc gcaggcatgt gggtattatc cagtccatac atctccacac
     3841 agtgttaacc gcaacattta aagttcaata aggacggttt caattttatg tataaaacag
     3901 cattagtgat gattatcggg atcctcggat gcagtaaagc atttgcagcc actgaattac
     3961 agctccagca aaaacgcgtt atgcagttct gtgccaacgc cagtcttcca ttgttagttg
     4021 cgggtacaac gtacgcgaat acgtctgaca acgggcgacc agaaaaagaa agattggcaa
     4081 tcctgaaaaa ttcagttgca agctcgacag cttataaaat ggcatctccc ggagttcaga
     4141 ttgccatgat gagcgtggtg gaagatattg ccgatccgaa agaattagct ctacatcaaa
     4201 aagaagttag acgtcttggg gctagttatc tttccgacag cggtgtttca tgggcttcaa
     4261 aaactgtttc accatttaca gcctggtgta actttaaccg tttagaaagt taaggctggc
     4321 aaggcggttc ctgttcagga aagttacttt ccgtgcgggg accgtaagat acagataaag
     4381 cttaaggcct ttgaatttct ggaaagcatt tgaaaatttc attgcgcttg tctgatatga
     4441 aacagcccct gcatttctgc aaaggctgaa agagtgtgta cggactttgg aaggagtgat
     4501 gatatacgcc tgagccatcc catcaccgca gacagggtaa ttaaccctga ctaaagcttt
     4561 cacctattcc gtcgctcgca agcggctttc gcacactaac gaaggtatgg cctttcgtcg
     4621 gtgttaaatt tttcaaactt tgcttacttt gtcaaggaag gtgaccgcag tagtaggcac
     4681 aactacttta atctgtatga ataataaggt agaaccggcc agtgaatgaa tgctttccct
     4741 gaaatcaggc ggacaacctt caggaatatt gatagcaaga ctgaatctct cgctgcggcg
     4801 ataaacattt cgccataacc ctgaacgaga gtggttcatc catcatatac aggagcgtta
     4861 catgcgcaaa tttaagtaca taatctgtca ccagtgcgaa ggccacggga ccatggaaaa
     4921 cccggccttt gaaaatggat tcacccaatc agaaatggct gagtgggagc cagaaatgcg
     4981 tgaaaagtat tttgccggag catttgatgt tcgctgtgac gtttgtgccg gtgacggtaa
     5041 gctcagcgta ccaaacgtag cggctatgtc tttttcagaa cgacgggttc tggcagcacg
     5101 gcggcgtgat gagcgtcttc aggctgctga tgaacggctg tcccgccagg aacgagcaat
     5161 ggggtactga ggtgttacag caaagatgaa aatgacgaag ccaaaaccgg taagccggat
     5221 gaaaagaccg tttattagtg agcttgcaga gctgggttta tcgggtgaaa ttctttcaat
     5281 tcgaactgac ggcatgcagg ggacgctcat acccgatctt accgcagggg tgagtgaagc
     5341 aggccgtgtg aaaaccggca actgggtaat ctctgacaag agaaccttcg accatgtcgt
     5401 tctgtgtggg gtcgtctcag aaaacggaaa ataaagcacg ctaagccggt ggcagcggtc
     5461 gcaatggcct aaacttcccc gcaccgacct tggcgctgct gcgccatagg taatcgccgg
     5521 tcaggttgat gtgctcccac cccagcggcg acagatattg caacaatgtg tcgtccagcg
     5581 ccgtgccgtt gccacgcaaa gcactggtgg cacgctccag atataccgtg ttccacaaca
     5641 cgatggccgc cgtcaccaga ttgaggccgc tggcccggta gcgctgctgc tcaaaactgc
     5701 ggtcgcggat ttcacccaat cggtagaaga agaccgccct ggccagcgcg ttgcgcgcct
     5761 cgcccttatt cagccccgca tggacgcggc ggcgcagctc cacgctttgc agccaatcca
     5821 aaatgaacag cgtgcgctcg atgcgcccca gctcgcgcaa cgccacggcc aagccgttct
     5881 ggcgcgggta gctgccgagt ttgcgcagca tcagcgaagc cgttaccgtg ccttgcttga
     5941 tggaggtggc cagccgcaga atttcatccc aatgggcgcg tatttgcttg atgttcagcc
     6001 tgtcgctgct aatcatcggc ttgagcgcgt cataggcggc atcgcccttg gggatgaata
     6061 gcttggtttc gcccaagtca cggatacgcg gcgcgaagcg aaatcccagc aaatgcatca
     6121 agccaaacac gtgatcggtg aagcctgccg tgtcggtgta gtgttcctcg atgcgcaagt
     6181 ccgactcgtg gtacagcagg ccatcaagca cgtaagttga atcacgaatg cccacgttga
     6241 ccaccttggc actgaagggc gcgtactggt cggagatatg ggtgtagaaa gtccgtcctg
     6301 gactgcttcc atacttcggg ttgatatgac cagtgctttc tgctttgctg ccggttctga
     6361 agttctggcc gtccgacgat gacgtggtgc cgtcacccca gttgccggcg aagggttgcc
     6421 gaaactgcgc attcaccagc tcggccagcg ccgtcgaata ggtttcatcg cggatgtgcc
     6481 aggcttgcag ccaagacagc ttggcgtagg tggtgccagg gcaggactcg gccattttgg
     6541 tcagacccag gttgatcgcg tcggccagga tcgtcgtcaa cagcaaggtt ttgtccttgg
     6601 ccgtgtcgct ggtcttcagg tgtgtgaagt ggcgggtgaa gcccgtccat tcatcgacct
     6661 ccatcagcaa ctcggtgatt ttgaggtgcg gcagcagcat agctgtctgg tcgatcatgg
     6721 cttgcgcggc gtctggtact gccgcgtcca gcggcgtgat cttcaggcct gacgcggtgg
     6781 tgatgatggc atccggtaag tcgttggccg cagccatgcg gttgactgtg gcgagttgcg
     6841 cctccaacaa ttccaaccgg tcatgcaggt attggtcgca gtcggtggcc actgccagcg
     6901 gcaattcgct ggccagcttc aaagtggcga acttctcgac cggcaccagg tattcgtcga
     6961 agtccttgaa ctggcgagaa ccctgcaccc agacatcacc ggagcgcagc gcgttcttca
     7021 gctccgacag ggcgcataac tcgtagtaac gccggtcgat gccgtcgtcg gtcagaacca
     7081 gctttgccca gcgcggcttg atgaatgcgg ttggcgcatc ggcgggcacc ttgcgcgcgc
     7141 tgtcgctgtt catgccgcgc agcatgtcga tggcatcgag cacacccttg gcggcgggcg
     7201 cagcccgcaa tttgagcacg cccaggaact gcggcgcgta gcggcgtagc gtggcatagc
     7261 tttcaccgat gtggtgcagg aaatcaaagt cggcaggccg cgccaatgtt tgcgcttcgg
     7321 tgacgctggc ggcgaaggtg tcccagggca taacggcctc gatggcggcg aacggatcgc
     7381 tgccgctttg cttggcctca atcaacgctt gaccgatgcg cccatacatc cgcaccttgt
     7441 cgttgatcgc cttgccggaa gcctggaact gctgctgatg cttgttcttg gccgcgttga
     7501 acagcttgcc gatgatgcga tcgtgaaggt cgatgatttc atcggtgacg gtggccatgc
     7561 cttcgatggc cagcgctacc agcgtggcat agcgtcgttg cacctcgaac tttgccagat
     7621 cagcaggcgt catctggcca ccttcacgag cgattttgag caggcggttc tggtgaacct
     7681 gccgctcgat gcctgcgggc agatcaagtg cttgccagga tttcaggcgc tcaatatgtt
     7741 cgagcatgtg gcgagagttc ggtttggcag gcgactggcg cagccatgcc agccacgtca
     7801 ctttactgcc gtccttgcgc ttgagaagtt cgtccaggcg ctgacggtgg ggtgataaca
     7861 aagaatcggt cagcgccgcg taaatgcgtc ggttggcacg ggtgatggcc tcggcgcttg
     7921 cgcgctcgat ggcattcatg gcgggcagga taatgctctg ccgccgcaga ttctcgacaa
     7981 gtgcgctcgc cagcacgatg cctttgtcgg tctgcaaggc cagctcggtc aatgtatgca
     8041 cggcttgccg atagtggctc atggtgaagg gcttgaaccc aaaaaccgtt tgcagctcga
     8101 ccaagtgctc ccgccgtgtc tgttcgcgct ggccgtactc gctccaactt tccactggca
     8161 tcttgagttg cgcggccacc atgcgcaaca ggggcggaaa cggaggctca tcgacgccca
     8221 aaaaggtgcc agggaatcgc aagtagcaaa gctgcacagc gaagcccaat cgattcgcgg
     8281 cgccgcgacg ctgacggatc accgacaggt cggtttcgtt gaacgtgtag tgccgtatca
     8341 gttcgtcttt ggcatctggc agtgccagca ggctttcgcg ctcggtggcg gacaggattg
     8401 agcggcgtgg catggtcagt cttcccgcag gtactggtac aaggtttcgc ggctgatgcc
     8461 gaagtcacgg gccaccaagg ttttttggtc gcctgccgca actcgccgtt tcaactcggc
     8521 aatttgttcg ctgttcagcg atttctttcg tccccggtag gcaccgcgct gcttggccag
     8581 cacgattccc tcgcgctgac gttcgcggat cagggcgcgc tcgaactcag cgaaggctcc
     8641 catgaccgac agcatcagat tggccatcgg tgagtcctcg ccggtgaact tcagcccttc
     8701 tttgacgaac tccatgcgca cgccccgttg tgtcagccct tggacgatgc ggcgcaggtc
     8761 atcaaggttg cgtgccagcc tgtccatgct atgcaccacc acggtgtcgc cctcgcggac
     8821 gaaggccagc agcctttcca gctcgggacg ctgggtgtcc ttgccagaag ccttgtcggt
     8881 gaacacccgc gccacctgaa caccctccaa ttgccgttcc gggttctggt cgaagctgct
     8941 gacgcggaca tagccgatgc gttgaccttg caagatgcct ccaaaggcaa aagtgtcagg
     9001 atgaaatcta ttacctttga cggaatatgt caatcaatag gaaatttaac tctattctga
     9061 catcgtttgc acat
//
LOCUS       DAPGOF010000192         8964 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.193, whole genome shotgun sequence.
ACCESSION   DAPGOF010000192 DAPGOF010000000
VERSION     DAPGOF010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8964
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.193"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..719
                     /gene="yneK"
                     /locus_tag="RHV13_004055"
     CDS             <1..719
                     /gene="yneK"
                     /locus_tag="RHV13_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HDV1243528.1"
                     /translation="PVTMGRVFQTGVERVLFLFLNDFIEQFPMINPGIPIKRAHTPHI
                     EPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSEVHRWFVNKDPNLITAIF
                     DITDSELKEDLLQSLMDSLGSNKHVLPEVCICFLSLLAEQDFPHFQDLFLFFANMLLH
                     YHQFMNPNESDLNDVLMPASLSDDKIIKHMARKTLKLFVKNETPPKVTHEDLVKNRPR
                     SPVRPPIPATSKTPDLPERH"
     gene            869..2059
                     /gene="ydeA"
                     /locus_tag="RHV13_004056"
     CDS             869..2059
                     /gene="ydeA"
                     /locus_tag="RHV13_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HDV1243529.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(2083..2748)
                     /gene="marC"
                     /locus_tag="RHV13_004057"
     CDS             complement(2083..2748)
                     /gene="marC"
                     /locus_tag="RHV13_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HDV1243530.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            2960..3394
                     /gene="marR"
                     /locus_tag="RHV13_004058"
     CDS             2960..3394
                     /gene="marR"
                     /locus_tag="RHV13_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HDV1243531.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            3414..3797
                     /gene="marA"
                     /locus_tag="RHV13_004059"
     CDS             3414..3797
                     /gene="marA"
                     /locus_tag="RHV13_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HDV1243532.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            3829..4047
                     /gene="marB"
                     /locus_tag="RHV13_004060"
     CDS             3829..4047
                     /gene="marB"
                     /locus_tag="RHV13_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HDV1243533.1"
                     /translation="MKPLLSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(4104..5543)
                     /locus_tag="RHV13_004061"
     CDS             complement(4104..5543)
                     /locus_tag="RHV13_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDV1243534.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     RKVTEGIIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAGTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYTPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITSEDKAILARGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
     gene            complement(5568..7241)
                     /locus_tag="RHV13_004062"
     CDS             complement(5568..7241)
                     /locus_tag="RHV13_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HDV1243535.1"
                     /translation="MNIKTLNVSLLSFSILTALFPLNAMATKLTIEQRLELLENELSK
                     NKQELKATQNELGVYKSRLSTLQKSITENKYKSASLAEISATSPVADNIKNENGEQNS
                     SAAAHTINGSQQFAVIESKGDKTTIESVTMKDISKYIKDDIGFSYQGYFRSGWGTGNH
                     GSPQTYAAGSLGRFGNEMSGWFDLTLNQRVYNQDGKTANAVVTYDGNVGEQYNDAWFG
                     DSANENIMQFSDIYLTTRGFLPFAPEADFWVGKHKLPQYEIQMLDWKTLTTDVAAGVG
                     IENWALGVGLFDMSLSRDDVDVYSRDFTRTSQMNTNSVDVRYRNIPLWDDATLSLMAK
                     YSAPNKTDQQQDNENDDSYFEMKDSWMLTSILRQNLQRDTFNEFTLQVANNSYASSFA
                     SFSDASNTMAHGRYYYGDHTNGIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSYES
                     GAHSDFDSIRTVIRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHALK
                     VGESILGSRPEIRFYGTYINILDNELSNFKFNENSKDEFMAGIQAEVWW"
     gene            complement(7297..7608)
                     /locus_tag="RHV13_004063"
     CDS             complement(7297..7608)
                     /locus_tag="RHV13_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDV1243536.1"
                     /translation="MFADEELVMELLVNAGQARSDAMEAIRCAGKKDWQGATQLMASS
                     ESACLQAHKIQTALISQDEGCGKIKVNLILVHAQDHLMNAMLCQDLAREIISLRKELH
                     A"
     gene            complement(7636..8958)
                     /locus_tag="RHV13_004064"
     CDS             complement(7636..8958)
                     /locus_tag="RHV13_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDV1243537.1"
                     /translation="MGLMASFERGMERFLVPVAIKLNSQKHVAAVRDGFVFTFPIIMA
                     SSLIILINFAILSPDGFIAGLLHLNSIFPNLEKAQAIFTPVMNGSVNIMSIMIAFLVA
                     RNMAISYEQDDLLCGLTAIGAFFIVYTPYQMIEGQAFLTTKYLGAQGLFVAVIVALIT
                     SEIFCRLARNPKITITMPAAVPPAVARSFKVLLPIFFVMVFFSTLNYCLILISPAGLN
                     DLIYTLIQTPLKHMGTNIFAVIILGAVGNFLWVLGIHGPNTTSAIRETVFSEANLENL
                     SWAAQHGTTWGAPYPITLTSINDAFANCGGSGMTLGLLLAIFIASKRAEYRDLAKMSF
                     IPGIFNINEPIMFGLPIVLNPIMMVPFILVPIVNCAIGYFFVSMEIIPPVAYAVPWTT
                     PGPLIAFLGTGGNWLALLVGFLCLGVATMIYLPFVIAANKVNNLTTNG"
BASE COUNT         2451 a         2138 c         1915 g         2460 t
ORIGIN      
        1 agcctgtcac tatggggcgc gtatttcaga ccggcgtcga gcgtgtgctc tttctgtttc
       61 ttaatgattt tattgaacaa tttccaatga tcaaccctgg catccccatc aaaagagcgc
      121 atacaccaca tattgaaccc ctgcctccgg atcaccatac cgctgcagat tacttacgcc
      181 agtttgattt gcttgtcctg aattttatct ctcgcggtaa ttttgtcata cttccccgat
      241 tatggaataa ctctgaggtt cacagatggt tcgtcaataa agatcctaac ttgatcaccg
      301 ccattttcga cataacggac agcgaattaa aagaggattt attgcaaagc ctgatggatt
      361 cattaggttc taacaaacat gtactacccg aagtctgcat ctgcttttta tcccttttag
      421 cagaacaaga ttttccacat tttcaggact tgtttttatt tttcgccaat atgttgttac
      481 actatcacca atttatgaat cccaatgaaa gtgatttgaa tgacgtgttg atgccagcat
      541 cattaagtga tgataaaatt atcaaacata tggcgcgcaa gaccctcaaa ctttttgtaa
      601 aaaatgaaac gccgccaaaa gttactcatg aagacctggt gaaaaacagg cctcgctccc
      661 ctgtcagacc acctataccc gcaacctcca aaacgccaga cctccctgaa cgtcattaaa
      721 ccgtgatgtt accaactctc tgacgcggaa cagaatcagc gtcagagaaa cggaaaacgc
      781 gatccagatc acaaatgcat tgtattcaca tcattaaccg ttttaagatc atttcatcac
      841 tttttcgcaa ctcacccgat aatctgttat gacaacaaac actgtttccc gcaaagtggc
      901 gtggctacgg gtcgttacgc tggcagtcgc cgccttcatc ttcaacacca ccgaatttgt
      961 ccctgttggc ctgctctctg acattgcgca aagttttcac atgcaaaccg ctcaggtcgg
     1021 catcatgttg accatttacg catgggtagt agcgctaatg tcattgcctt ttatgttaat
     1081 gaccagccag gttgaacggc gcaaattact gatctgcctg tttgtggtgt ttattgccag
     1141 ccacgtactg tcgtttttat cgtggagctt taccgttctg gtgatcagtc gcattggtgt
     1201 ggcttttgca catgcgattt tctggtcgat tacggcgtcc ctggcgatcc gtatggctcc
     1261 ggccgggaag cgagcacagg cattgagttt aattgccaca ggtacagcac tggcaatggt
     1321 attaggatta cctctcgggc gcattgtggg gcagtatttc ggttggcgaa tgaccttctt
     1381 cgcgattggt atcggggcgc ttattaccct tttgtgcctg attaagttac ttcccttact
     1441 gcccagtgag cattccggtt cattgaaaag cctcccgcta ttgttccgcc gcccggcatt
     1501 gatgagcatt tatttgttaa ctgtggtggt tgtcaccgcc cattacacgg catacagcta
     1561 tattgagcct tttgtgcaaa acattgcggg attcagcgcc aactttgcca cggcattact
     1621 gttattactc ggtggtgcgg gcattattgg cagcgtgatt ttcggtaaac tgggtaatca
     1681 atatgcgtct gcgttggtaa gtacggcgat tgcgctgttg ctggtgtgtc tggcactgct
     1741 gctacctgcg gcgaacagtg aaatacacct cggggtgctg agtattttct gggggatcgc
     1801 gatgatgatc atcgggcttg gtatgcaggt taaagtgctg gcgctggcac cagatgccac
     1861 cgacgtcgcg atggcgctat tctccgggat atttaatatt ggaatcgggg cgggtgcatt
     1921 ggtaggtaat caggtgagtc tgcactggtc aatgtcgatg attggttatg tgggcgcggt
     1981 gcctgctttt gccgcgttaa tatggtcaat cattatattt cgccgctggc cagtgacact
     2041 cgaagaacag acgcaatagt tgaaaggccc atccgggcct ttttaatggt acgttttaat
     2101 gatttccagg atgccgttaa taataaactg cacacccata cataccagca ggaaccccat
     2161 caggcgggag atcgcttcaa tgccaccctt gcccaccagc cgcataattg cgccggagct
     2221 gcgtaggctt ccccacaaaa taaccgccac caggaaaaag atcagcggcg gcgcaaccat
     2281 cagtacccaa tcagcgaagg ttgaactctg acgcactgtg gacgccgagc taataatcat
     2341 cgctatggtt cccggaccag cggtacttgg cattgccagt ggcacaaagg caatattggc
     2401 actgggttca tcttccagct cttccgactt gcttttcgcc tccggtgaat caatcgcttt
     2461 ctgttgcgga aaaagcatcc gaaaaccgat aaacgcgacg attaagccgc ctgcaattcg
     2521 cagaccggga atcgaaatgc caaatgtatc catcaccagt tgcccggcgt aatacgccac
     2581 catcatgatg gcaaagacgt acaccgaggc catcaacgac tgacgattac gttcggcact
     2641 gttcatgttg cctgccaggc caagaaataa cgcgacagtt gttaatgggt tagctaacgg
     2701 cagtaacacc accagcccca ggccaattgc tttaaacaaa tctaacattg gtggttgtta
     2761 tcctgtgtat ctgggttatc agcgaaaagt ataaggggta aacaaggata aagtgtcact
     2821 ctttagctaa ccttgcatcg cattgaccaa aacttgaacc gatttagcaa aacgtggcat
     2881 cggtcaattc attcatttga cttatacttg cctgggcaat attatcccct gcaactaatt
     2941 acttgccagg gcaactaatg tgaaaagtac cagcgatctg ttcaatgaaa ttattccatt
     3001 gggtcgctta atccatatgg ttaatcagaa gaaagatcgt ctgcttaacg agtatctgtc
     3061 tccgctggat attactgcgg cacagtttaa ggtgctctgc tctatccgct gcgcggcgtg
     3121 tattactccg gttgaactga aaaaagtgtt gtcggtcgac ctgggagcac tgacccgtat
     3181 gctggatcgc ctggtctgta aaggctgggt tgaaaggttg ccgaacccga atgataagcg
     3241 cggcgtactg gtaaaactta ccaccagcgg cgcggcaata tgtgaacaat gccatcaatt
     3301 agttggccag gacctgcatc aagaattaac aaaaaacctg acggcggacg aagtggcaac
     3361 acttgagcat ttgcttaaga aagtcctgcc gtaaacaaaa aagaggtatg acgatgtcca
     3421 gacgcaatac tgacgctatt accattcata gcattttgga ctggatcgag gacaacctgg
     3481 aatcgccact gtcactggag aaagtgtcag agcgttcggg ttactccaaa tggcacctgc
     3541 aacggatgtt taaaaaagaa accggtcatt cattaggcca atacatccgc agccgtaaga
     3601 tgacggaaat cgcgcaaaag ctgaaggaaa gtaacgagcc gatactctat ctggcagaac
     3661 gatatggctt cgagtcgcaa caaactctga cccgaacctt caaaaattac tttgatgttc
     3721 cgccgcataa ataccggatg accaatatgc agggcgaatc gcgcttttta catccattaa
     3781 atcattacaa cagctagttg aaaacgtgac aacgtcactg aggcaatcat gaaaccactt
     3841 ttatccgcaa tagcagctgc gcttattctc ttttccgcgc agggtgttgc ggaacaaacc
     3901 acgcagccag ttgttacttc ttgtgccaat gtcgtggttg ttcccccatc gcaggaacaa
     3961 ccaccgtttg atttaaatca catgggtact ggcagtgata agtcggatgc gctcggcgtg
     4021 ccctattata atcaacacgc tatgtagtta ttgtcaggct ctgatcacag agcctgacat
     4081 ctccgtaata tcttcctgaa gtctcaaata ttttcgccat tgttggcgat gagatcccga
     4141 taccagtaaa acgacgcttt acgtatccgt tccagcgttc ccttcccttc gttgtcttta
     4201 tcgacataaa tcattccgta gcgctttttc atttcccctg ttccagcaga aaccaggtca
     4261 atgcagcccc acggggtgta gccaattaag tcaacaccat cttcgactac ggcttttttc
     4321 atttcccgaa tatgggaagc aaagtaatca atgcgatagt gatcgtttac cgtgccgtca
     4381 gcttgtctct ggtcaactgc accaaatcca ttttcgacaa taaacagcgg caactggaaa
     4441 tgatcccaga accagttgag tgaataacgt agccctgccg gatcaatttg ccatccccat
     4501 tcggatgtat cgatataagg gttgctgacc aaatctcgtg gttcaacata atccagttgc
     4561 gggttatcgg gcgaaaattg tgtcgtaaaa gacatgtagt agctaaaacc gataaagtcg
     4621 acacaacctc tggcaagaat cgctttatct tctgatgtga tatcgaggtt gaatcctttc
     4681 ctggcaaagt aattcagcat atgttgcgga taatatccac gagcatgaac atcagtaaac
     4741 cagtaacgac gatgcatcgc tttcgtggcc atcatcatat cgttgggtgc acacgtcaga
     4801 ggatagatgg ggcacatggc gatcatacag ccgatattaa attcaggatt gatcgatttt
     4861 ccagtctgta ccgcaagggc actggcaact aactcgtaat gtaccgcctg gtacattatt
     4921 tgttcgcgct cattgatatc ttcctctggc gtatacaaga taccggaatt agtaaatgga
     4981 cataggcttt cgctgaaatt cacctgatta ttgatttcgt taaacgtcat ccagtacttt
     5041 actttttctt tatagcgcgt gaagaccgtt gatgcgaagc ggataaaaaa gtcgatcagt
     5101 ttacggtttc gccagccacc atattttgtc accagatgat aaggcatctc aaaatgtgaa
     5161 agcgtcacca caggttccat tccctgctta aggcattcat cgaacagatc atcataaaat
     5221 tgtaaaccct cttcattcgg ctcctgttcg tcaccttgcg gaaagattcg tgtcccggca
     5281 atggaagttc gaaagcattt gaatcccatc tcggcaaata actgaatatc tgttttatag
     5341 cgatgataaa aatcaattgc ttcatgattg ggataattaa gcccgtcgat aatgccttct
     5401 gtcactttac gcggcacccc gtgagcgcca gcagtcatca catcagcgat actgatgcct
     5461 tttcctcctt cattccagcc accttccaac tgatgcgcgg ctaccgcacc gccccataaa
     5521 aaaccttttt taaatcctga catacacaac tccttaacta aaatagatta ccaccagact
     5581 tccgcctgga tgccggccat aaactcgtct ttgctgttct cattaaactt aaaattcgat
     5641 aattcgttat ccagaatatt gatatacgtc ccatagaagc gaatttctgg tcgtgaacct
     5701 aaaatacttt cacccacttt caatgcatgc cagagtgtcg ttttataagc cgattcattt
     5761 agtgttacac cctgctgtgt tttgttctgt tgcttaaacc agcctaattc aagccccgtc
     5821 tgattccatg tattccagat ccaggccggt cttattacgg tgcgaatact gtcaaaatca
     5881 ctatgagcgc cactttcata actataaaca tcttcgccgt gagaatagac aagcgcgtta
     5941 gccataataa tattgtcagt gagatacatc tcgccctgag agattaaacg ccaggcgatc
     6001 ccattggtat ggtcaccata gtaatagcga ccgtgcgcca tcgtgttact ggcatctgag
     6061 aagctggcaa aactgctggc ataggaatta ttggcaacct gtaacgtaaa ttcattaaac
     6121 gtatcgcgtt gcaggttttg ccgtaaaata gaagtcagca tccagctatc tttcatttca
     6181 aaatagctgt cgtcattttc attatcttgt tgttgatccg ttttattagg tgcggaatat
     6241 ttagccatta atgataatgt cgcatcatcc cataacggga tattgcgata acgaacatcc
     6301 acagaattgg tattcatctg gctggtacgc gtaaaatcac gagagtaaac atcgacatca
     6361 tcacggctta aggacatatc aaacagccct acaccaagcg cccagttttc aatccccaca
     6421 cctgcggcga catccgtggt taaggttttc cagtccagca tctggatctc atattgcggg
     6481 agtttatgtt tgccgaccca gaagtctgcc tctggcgcga agggtaaaaa acctcgcgtt
     6541 gtcagataaa tatcactgaa ctgcatgata ttttcattgg cactgtcacc aaaccaggca
     6601 tcgttatact gctcacccac gttgccatca taggtaacga ccgcatttgc cgttttaccg
     6661 tcctgattat agacacgctg atttaaggtc aggtcaaacc aaccactcat ctcgttacca
     6721 aaacgtccca gagaacccgc tgcataagtt tgtggtgagc cgtgattacc ggtaccccaa
     6781 cctgagcgaa agtacccctg atagctgaac ccaatatcat cttttatata tttactgata
     6841 tctttcatgg tcacgctttc gatagtggtt ttgtcgcctt tactttcaat aacagcaaat
     6901 tgctgcgatc catttatagt atgcgctgcg gcagacgagt tctgttcacc gttttcattt
     6961 ttgatgttgt cagcgacggg agatgtcgct gatatttcag caagcgaggc tgatttatat
     7021 ttgttttccg taatactttt ttgtaaggtc gaaagtctgg atttatatac tcccagttca
     7081 ttctgtgttg ctttcagctc ttgtttattt ttcgacaatt cattttcaag caattcaagg
     7141 cgctgctcta tggttaattt tgttgccatc gcgttcaacg gaaacaatgc cgtaagaata
     7201 gaaaaagaca aaaggctgac gttaagcgtc tttatattca tgacatatcc ttaagagtgt
     7261 aatagtttcc tccagctaac gcactggagg aaaaattcag gcatggagtt cttttcttaa
     7321 tgaaataatt tccctggcta aatcctggca taacatcgca ttcatcaaat gatcctgtgc
     7381 atgtaccaga attaaattaa cttttatttt gccacagcct tcatcctgac taattaacgc
     7441 ggtctgaatc ttatgtgcct gaagacaggc agattcagaa ctggccatta gctgagttgc
     7501 gccctgccag tcttttttcc cggcacaacg tatggcttcc atcgcatcag aacgagcctg
     7561 tcccgcgttg accagtaact ccataacaag ttcttcatct gcaaacatca acatctcctt
     7621 tctgtgtgaa tgggattatc cgttagttgt taagttattg accttgttgg cggcaataac
     7681 aaaaggtaaa tagatcattg tcgccacacc taaacataaa aaaccaacca gtaacgccag
     7741 ccagtttccc ccggttccga ggaaagcaat taaaggtccg ggcgtagtcc agggcacggc
     7801 ataagcaacc ggtggaataa tttccatcga aacaaagaag taaccaatgg cacagttaac
     7861 aatgggaacc agaataaacg gcaccatcat aatggggtta agtacaatag gaaggccgaa
     7921 cattatcggt tcattgatat tgaaaatacc ggggataaat gacatttttg ccagatcacg
     7981 gtattccgca cgcttagatg cgataaaaat agccaacaat aaccccaacg tcatacctga
     8041 accgccgcag ttggcgaatg catcattaat agaagtcaag gtaatcggat atggcgcgcc
     8101 ccaggtagtg ccgtgttgag cggcccagga gagattctcc agattagcct cagaaaaaac
     8161 ggtttctcga attgccgacg tggtattagg tccgtggatc cccagcaccc agaggaaatt
     8221 acccacagcc cccaggataa ttaccgcaaa gatattcgtt cccatatgtt tgagcggcgt
     8281 ctggattaat gtgtaaatga gatcgtttaa tcctgccggg gatatcagta tcaggcaata
     8341 attaagtgtg gaaaagaaca ccataacaaa aaatattggc aataaaactt taaatgaacg
     8401 cgctaccgca ggaggtacag ctgccggcat cgttatggtg atttttgggt ttcgagctaa
     8461 gcgacaaaat atttcactgg tgatcaatgc aacgataaca gcaacaaaca acccctgcgc
     8521 gccgagatat ttggtcgtca ggaatgcttg tccttctatc atctgatatg gtgtataaac
     8581 aataaaaaat gctcctattg ccgttaatcc gcataaaaga tcatcttgct catagctaat
     8641 cgccatattt ctggcgacca ggaaagcaat cataattgac atgatattta cagaaccatt
     8701 cattaccgga gtaaaaatag cttgtgcttt ttcaaggttg gggaaaatgc tgttcagatg
     8761 cagtaatccg gcaataaaac cgtctggcga taatatggca aagttaatta atataattaa
     8821 tgaacttgcc atgataattg gaaacgtaaa aacgaatcca tctctcaccg ctgcaacatg
     8881 tttttgtgag tttaacttga tagcaactgg aacaagaaaa cgttccattc cacgttcgaa
     8941 tgaggccatt aatcccattt ttta
//
LOCUS       DAPGOF010000193         8737 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.194, whole genome shotgun sequence.
ACCESSION   DAPGOF010000193 DAPGOF010000000
VERSION     DAPGOF010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8737
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.194"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1..241
                     /locus_tag="RHV13_004065"
                     /pseudo
     CDS             1..241
                     /locus_tag="RHV13_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284754.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            251..1192
                     /locus_tag="RHV13_004066"
     CDS             251..1192
                     /locus_tag="RHV13_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDV1243538.1"
                     /translation="MAKRKEYIRVRLESCKTRGERLMAFGLIDTIDPEYIQELEDHLA
                     KVSHRKMLEDNDRKVKERNRLLSEMKKNSARLTERIILSEKEEPDEEASLPILVRRYI
                     PGGRAAEFAIYARSNATLPPLEIIEGFLGYPVGGRERSQEDQPKSPPAAIMVAMKTDG
                     IEEKELQPNIPDETTVPGKRKGSKTTVETRPDQASFAEAVRNNCYDQCVITGSRLRQR
                     TEAAHLMEHRNGGVDHYTNGLLLRHDIHDLFDANMIAIHPVTLEVNILAEALAVDHDL
                     AAYQGEFIKPLRKPINTEFLKHRWQVFQHRNQTEQQA"
     gene            complement(1300..1818)
                     /locus_tag="RHV13_004067"
     CDS             complement(1300..1818)
                     /locus_tag="RHV13_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243539.1"
                     /translation="MQKSPLHEEVCVPEETTAGLWGPLPDSHVVLFLDFDGVCHRCKN
                     ETFERMPLVEKLLDKCPSMVIVISSSWRECASITYLKSLFRLPYRDKVIGATDSVYLK
                     PNQSGVRAAECEDFVFSHRVKAFICLDDDESLFPVGYPHLHKTNYYTGLTESDLAALN
                     TRYHLLMKRWAS"
     gene            complement(1949..2488)
                     /locus_tag="RHV13_004068"
     CDS             complement(1949..2488)
                     /locus_tag="RHV13_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HDV1243540.1"
                     /translation="MFTPPADDVKPIPVPDEIYTQCITDAARFFGIDAELVFTLFDNE
                     GGKVGTFSRNTNGTYDIGPMQINSSNLPEIKKHFPTVTWRVLAYDACASFWVGTWWLY
                     RKIVDRKGNVFEGIADYNSKTPKVRAKYIFNFMVKYNRRIQQRNGMGELYQWTQQPPR
                     YNGHIAKNVPEQNPTPVVK"
     gene            2636..3385
                     /locus_tag="RHV13_004069"
     CDS             2636..3385
                     /locus_tag="RHV13_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HDV1243541.1"
                     /translation="MKKIIKASVLLLSLSTAFTMNAEPVNTMVLPDAARDKLKAIGLS
                     IEHVEPSPVKDIFTVISREGVSYVSKDGDYIFTGSLFHVKGKDVVNTTEQAILMGVRE
                     FASKTKSIDYKSPNEKYRLAIFTDITCGYCQKLHHDLKSYLDAGISIKFLAFPRAGLN
                     SVVAGNMAKIWCSAKPNEALDAAMNPVSTIPEGRPDEACLNIIKSHFQVASTIPLQGT
                     PTMVTLSGKPQLFTGWLSPENLVTQMGAAQK"
     gene            3410..3802
                     /locus_tag="RHV13_004070"
     CDS             3410..3802
                     /locus_tag="RHV13_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243542.1"
                     /translation="MKSTLLSTLMLVAVGVQPVFAAQCQYGACGTENDPGIVFLMSSV
                     QMDQGEQLKELSGVATTGDTISKKMESYLGNKKLKVNTDSTRKGPGNTTIRPTDELAP
                     TRQLKVIRPELVKNPDSQLVVAFNERLA"
     gene            3836..4258
                     /locus_tag="RHV13_004071"
     CDS             3836..4258
                     /locus_tag="RHV13_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243543.1"
                     /translation="MSILSNFVIRATGIPDKKYLRDPVIKRCYERLSRRVPALMTGWL
                     LCILGSGYVSMALEQPDSTVLLSLLLLYVMSGILLMQFQYMYSERSIGYKFYLEVLMN
                     AAASTQHKEQLQYLIINKPNSITMGDLYRLYDFNGGGR"
     gene            complement(4318..4929)
                     /locus_tag="RHV13_004072"
     CDS             complement(4318..4929)
                     /locus_tag="RHV13_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243544.1"
                     /translation="MIRQGFILTTAMLLSGCGYHFANQVDAYDLMPRPVTNKRFQIVP
                     PDESIQSRMFSGRFADGLAKKGVIISTHQPDYVLRFRMSSSQENMQYSEQLLTGVTGY
                     VVDKKTTRTDKHGESHTDYDYRPVDGVIGTETMSQMHYMRQLDVEVYPSAKGAKQVLK
                     VSMQSNAPVPSDSIAYSAMIDAFTDKFDAPLRSGNYVAVIPWN"
     gene            5036..5845
                     /locus_tag="RHV13_004073"
     CDS             5036..5845
                     /locus_tag="RHV13_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDV1243545.1"
                     /translation="MSKEFYLKPMATILISAVIATAASALIAATYFKPKVLSEEEIGK
                     IAATYLVKNPHYLVEAGKALENQNVSASVERIIPYAPALLDTKETPNIGPDDADVAVI
                     EFFDYQCIYCMRVTPVVESVMNQSKDVKFFFKEFPIFAGSKPVSAMGAATGLHVYQNF
                     GAEAYRKYHNNLMAVAHTFMTSQRKFELSDFNTVVDKSGFNSTFSDREKNRYENVISG
                     NMQLGEALGITGTPGFIIMNMKKPNAATTTFIPGAMDAATLQGAIEKARGA"
     gene            complement(5891..7150)
                     /gene="umuC"
                     /locus_tag="RHV13_004074"
     CDS             complement(5891..7150)
                     /gene="umuC"
                     /locus_tag="RHV13_004074"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V subunit
                     UmuC"
                     /protein_id="HDV1243546.1"
                     /translation="MYMHVDINGAYAAFECAMDPKLSKKPLIIASNNDSSVIAMNKLA
                     KSVGIKRGTPIFKCRDLIQQHRIEVRSSNFTLYEDYSNRFHETLESFAPQSSRYSIDE
                     NFMLLKNMNKIIDYEDYGRLIRSTLLHNLSLTCGVGCSSTKTLAKLCTYASKRWAATG
                     GVVVLTDQARIRKLLGLISTREIWGIGQKISERLSAFGIITAGDFYNSDVRFLRKSFG
                     VEIERTWRELHGEPCFRLHESPPVRQQIIVSRSFGQRLNEIGKLHEAVSFFTARAAEQ
                     LRKDGSWTRQITVFIQSSNYAQGENRYSNCGIEPLTATQDTRDLVDAAMTILNRIYKP
                     GIAYAKAGVMLSAMTDGTEQLSLFDERPARPGSQALMKVMDRFNKEKRGALFLLGEGI
                     QQDFRMKQAMLSPRYTTRWDELLVVKA"
     gene            complement(7134..7568)
                     /gene="umuD"
                     /locus_tag="RHV13_004075"
     CDS             complement(7134..7568)
                     /gene="umuD"
                     /locus_tag="RHV13_004075"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HDV1243547.1"
                     /translation="MSVILEHISNKPYEMAPFFSDLLSCGVMSPCAGHEDNELNLHEY
                     VVRNRPSTFFVRAAGLSMINAGINDGAILVVDRSLTARHGSIVVALVDGEFTVKILHT
                     YPELLLMPSNPAYKPIRVNPESLEIWGVVTFALNQFSHVHAR"
     gene            7762..8379
                     /locus_tag="RHV13_004076"
     CDS             7762..8379
                     /locus_tag="RHV13_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243548.1"
                     /translation="MPLPPNFLSPEDQAEYDAYMSRFSEINHYYDYHTVPVIDWFFKQ
                     ATEALHHELWIPACTSFLNGIETSLMVTLKSLMLKPTVNDQHAAPELVDLEGISVMSN
                     ALLRKAKQEGVPVELLSFPAEQDMLVKVAAGRTPEAEIVRLRNNLCHGNILEFIMSVD
                     IGTSGPIRIFTPECCRELAHELSSISRNWVVGLHKYWVDNNLISP"
BASE COUNT         2456 a         2003 c         1984 g         2294 t
ORIGIN      
        1 taatttgctt aaaattgccc gctatcgtct caatgctggt tattcccatg ctgaaactgt
       61 actttcttta cgctgcacag gctcatacat tgagcgtacc gtaatgcatg cccccaaaaa
      121 tacccgtaaa aaactacgtg aacacctaca aaaatttgct ctagagacag agcagaatag
      181 cgaaaccgtt tattctaatt acaatactca gctccgtgta attcgagaaa aaaaacaaaa
      241 agaacatttg atggccaagc gtaaggagta cattcgcgtt cggctagaat cttgtaaaac
      301 aaggggggag cggttgatgg cgttcggtct aattgacact atcgatcccg aatatataca
      361 ggaactggaa gaccatctgg ctaaggttag tcatcgtaag atgttggagg ataatgaccg
      421 aaaggtaaaa gaacgcaata gactgttgag tgaaatgaaa aaaaattcag cccgtctcac
      481 cgaacgaata atcctgtcgg aaaaagagga accagatgaa gaagcctctc ttccaatact
      541 ggtacgtaga tatattcccg gcggacgtgc cgcagagttc gctatttatg cacgttcaaa
      601 cgctacatta cctccactag agatcattga gggttttcta gggtatccag ttggtggccg
      661 cgaaagatcg caggaagatc agcctaaatc tccaccggcc gcgattatgg tcgctatgaa
      721 gactgatggt atagaagaaa aggagcttca gccgaatatt cctgatgaaa ctactgtacc
      781 gggtaagcgt aagggtagta agacaactgt cgagacacga ccagatcagg cctcatttgc
      841 agaagccgtc cgcaacaact gttatgacca atgtgtgatt acaggctcta gactgcgcca
      901 gcgcactgag gctgctcatc tgatggagca tcgtaacgga ggagtagacc attatacaaa
      961 tgggttgctc ttacgccatg atatacacga tcttttcgac gcaaacatga ttgccattca
     1021 tccggtgacg ttggaggtta acattctcgc tgaagctttg gcggtagatc atgatttagc
     1081 ggcatatcaa ggagagttta ttaagccgct gcgtaaaccc atcaatactg aattcctaaa
     1141 gcaccgttgg caggtttttc agcaccggaa ccaaactgag cagcaagctt aagagaccgg
     1201 catgttttct tccaactgtg caatgctttg acccggggca gaaatggaga agcatagaac
     1261 tgataatgcg ggcatgctct ccgtaaaagt tactttaatt caggaagccc aacgtttcat
     1321 aagcagatga tacctggtgt taagagctgc aagatcagat tcggtgaggc cggtgtaata
     1381 attggttttg tgcaaatgtg gataaccgac cgggaataat gattcgtcgt catcaaggca
     1441 gataaatgct ttaactcgat gtgaaaatac aaagtcctca cactcagcag cccgcacacc
     1501 tgactgatta ggttttaaat ataccgaatc ggtagcacct atgactttgt ctctataagg
     1561 tagccggaaa agagatttca ggtaagttat actggcacat tcacgccacg aactggagat
     1621 gacaatcacc atggaagggc atttatccag taatttttcc accaatggca ttcgctcaaa
     1681 ggtttcattt ttgcacctgt gacaaacccc atcgaaatct aagaaaagca cgacatgcga
     1741 atcagggaga gggccccata aaccagctgt tgtttcttca ggaacacata cttcttcatg
     1801 aagagggctt ttctgcatca agaaagaaga gagaatctta aatatatttc ttgcagaaaa
     1861 agacaattta ttacccctga gtctatgcga attttttgcg ggggggtacc caagctactc
     1921 gatgatcttc tgtaatatca ggttgaattt atttaacaac aggagtcggg ttttgctccg
     1981 ggacattttt agctatatgg ccattgtatc gaggaggttg ttgggtccat tgataaagct
     2041 cacccatccc gttacgctgc tggatccggc gattgtactt taccatgaag ttaaatatgt
     2101 actttgcacg tacctttggg gttttgctgt tgtaatctgc aatcccttca aacacattgc
     2161 ccttgcgatc aacaattttt ctgtagagcc accatgttcc aacccagaaa ctagcgcaag
     2221 catcgtaagc cagaacccgc cacgttacag tcgggaaatg cttttttatt tcaggcagat
     2281 tggatgagtt gatctgcatc gggccaatat cataagtgcc gttagtattc ctgctgaaag
     2341 taccaacctt accgccttca ttgtcaaaca acgtaaaaac cagttcggca tcaattccga
     2401 agaagcgggc ggcatccgta atgcactgag tgtaaatctc atcaggaacg ggaataggtt
     2461 tcacatcatc tgctggtggt gtaaacacaa cgatttctcc actgaatcta cgttttatct
     2521 attctcccat atatctatcg actttctttc ggctttaccc gttgatttaa gcgtttaagc
     2581 aaaattctgg aatttttggg cgtgataata ttagtgctcg attcaaggag cataaatgaa
     2641 aaaaattatt aaggcatcgg tattacttct ttcattaagt accgccttca cgatgaatgc
     2701 cgagccggtt aatacaatgg tactgccaga tgcagcccgg gataagctca aagccattgg
     2761 gctttcaatc gagcacgtcg aaccctcgcc agtaaaagat atttttacgg tgatttccag
     2821 ggagggggta agttacgtga gcaaagacgg agattacatt tttaccggaa gcttattcca
     2881 cgtgaaaggt aaagatgtgg taaacaccac tgagcaggcc attttaatgg gcgtccggga
     2941 attcgcatcc aaaacaaaat ccatcgacta caaatcacca aacgaaaaat accggcttgc
     3001 tatctttacc gatataacct gtggatactg ccaaaaactg catcatgatc tgaagtctta
     3061 tcttgatgcc ggtatttcga ttaagtttct tgcattccca cgtgcaggac tgaactcggt
     3121 agtagccggg aacatggcca aaatctggtg ttcagcaaag ccaaacgaag ctctggatgc
     3181 agcgatgaat ccggtttcaa ctatcccgga aggacgtcct gatgaggctt gcttaaacat
     3241 tatcaaatcg catttccagg tggcctcaac aatcccgctg caaggtactc ctaccatggt
     3301 gacactaagc ggcaaaccgc agttatttac ggggtggctg agcccggaaa acctggtgac
     3361 ccaaatgggc gctgcgcaaa agtaactttt ccgactgtaa gaggtgtgta tgaaatctac
     3421 tttactgagt acgttaatgc tggttgctgt aggtgtacag cccgttttcg ctgcacaatg
     3481 tcagtacgga gcctgtggaa cggaaaacga tccaggaatc gtatttctga tgtcatcggt
     3541 acaaatggat caaggtgaac aactaaagga actctctggt gttgcgacca caggtgacac
     3601 catctctaaa aagatggaaa gctacctggg aaataaaaag ctcaaggtaa acaccgacag
     3661 tacccgcaag gggccgggta ataccaccat caggcctaca gatgagctcg ccccaacgag
     3721 gcaactaaaa gttatccggc ccgaactggt aaaaaatccc gattcgcagc tcgtggtagc
     3781 tttcaatgag cgcctggcct gatttattga ctgagagcgt ccagaaggaa aaaagatgtc
     3841 gatactcagt aacttcgtca tcagggcaac ggggatacct gacaaaaagt atctccggga
     3901 tcccgtaata aaacgttgtt atgaacgtct gagtcgtagg gtgcctgctc taatgacggg
     3961 ttggcttctg tgcattctgg gttcagggta tgtcagtatg gcactggaac agcctgatag
     4021 cactgtgtta ctcagtctgc tgctgttgta tgttatgtca ggcattttac tgatgcagtt
     4081 ccagtatatg tattcagagc gaagcatagg ctacaagttc tacctggaag tgcttatgaa
     4141 tgcagccgcc agtactcaac ataaagaaca gttacagtat ctaatcatta ataagcccaa
     4201 ttccatcacg atgggcgatc tttaccgact ttatgatttt aatgggggag ggcgatagcc
     4261 acgtttatgt gcgtgtccaa aagtcacttt tgaacattct cttcccgccg tctgctttca
     4321 gttccagggg atcactgcaa cataatttcc ggaacgcagc ggcgcatcaa atttatcagt
     4381 aaaggcgtcg atcattgctg aataagcaat gctgtctgac ggcacaggtg cattgctttg
     4441 catactcact ttcagaacct gctttgctcc tttcgctgac gggtatacct cgacatccag
     4501 ctgtcgcata tagtgcatct gcgacatcgt ctcagtgcct attaccccat cgaccggcct
     4561 gtagtcatag tcagtatgcg attcgccatg cttgtcagtt ctcgttgtct ttttatctac
     4621 aacgtagcct gtcaccccag taagaagctg ctcgctatac tgcatgttct cctgtgagct
     4681 gctcattcgg aacctgagca cataatctgg ctggtgagta gaaataatga ctcctttttt
     4741 tgctaatcca tcagcaaaac ggcctgaaaa cattctggac tgaatacttt cgtccggagg
     4801 gactatctga aaccttttat ttgtaacagg acgaggcatt agatcgtacg catctacctg
     4861 gtttgcgaaa tgatatccac aaccactcaa aagcattgcg gttgtgagta taaagccctg
     4921 cctaatcatc ctttaatctc caaaaagcat tgtttcacga ataattccga tttaccgggc
     4981 cagtataatc catcgcaggt taataactaa caggctgaaa agcataaagg tagctatgtc
     5041 taaagaattt tatttaaaac cgatggccac aattttaatt tctgcggtaa ttgctaccgc
     5101 tgcctcggcg ttaatcgcgg caacttattt taaaccgaag gtgctcagtg aggaagaaat
     5161 tggcaaaatt gccgctactt acctggtaaa aaatccccat tatttagtgg aagcaggtaa
     5221 ggcgctggaa aatcagaacg taagtgcctc agtggaacga ataattccat atgcgccagc
     5281 tctgctggat accaaggaga cgccaaacat cggcccggat gacgccgacg tcgcggtaat
     5341 cgagttcttc gactatcagt gtatttactg catgcgggtt acacctgttg ttgaatcggt
     5401 aatgaatcag agcaaagatg taaaattctt ctttaaagaa ttcccgattt ttgccggttc
     5461 aaaacctgta tctgccatgg gggccgctac gggtctgcac gtctatcaga atttcggtgc
     5521 tgaagcctat cgtaaatacc acaacaacct gatggctgtg gctcatacct tcatgacctc
     5581 tcaacgcaaa ttcgaactca gcgactttaa tactgtggtg gataagtcag ggtttaacag
     5641 cacgttttct gacagggaaa aaaaccgata tgaaaacgtg atctccggca acatgcagct
     5701 gggcgaagcg cttgggatca ccggcacacc cggcttcatc attatgaata tgaaaaagcc
     5761 gaatgcggcg actaccacat ttatccccgg ggctatggac gcagcaactt tgcagggcgc
     5821 aatcgaaaaa gctcgcgggg cctaaagtta ctttcacttt tgatgagtac ggccatgccg
     5881 gtcatggccg ctacgctttt acaacgagta attcatccca gcgggtcgta tatctcggtg
     5941 atagcatggc ctgtttcata cgaaaatcct gttgaatgcc ttcccccagc aaaaacagag
     6001 ctccgcgctt ttctttattg aaccggtcca taactttcat cagtgcctga ctgccaggcc
     6061 tggccgggcg ctcatcaaac aggctgagtt gctctgtgcc gtcagtcata gcggagagca
     6121 ttaccccagc tttcgcatac gctatcccgg gtttgtaaat gcggttcagg atggtcatgg
     6181 ctgcatcaac cagatcgcgg gtgtcctgtg tagcggtaag cggctcgata ccacaattgc
     6241 tgtagcggtt ttcaccttgc gcataattgc tgctctgaat gaagactgtg atttgacggg
     6301 tccacgagcc atccttgcgc agctgctctg ccgcccgggc ggtaaaaaat gaaactgcct
     6361 catgtagctt gcctatctca ttaaggcgct gaccaaaact tctggaaacg ataatctgtt
     6421 gccggacagg aggggattca tgcagccgga aacatggttc gccatgcagt tcccgccagg
     6481 tacgctcgat ttcaacgcca aagcttttac gtaaaaagcg aacgtcgcta ttataaaaat
     6541 caccggcagt gatgatcccg aaagccgaca ggcgttcact gatcttttga ccaatacccc
     6601 atatttctct ggtactgata aggcccagga gcttcctgat cctggcctga tcagtcaaaa
     6661 ctacaacccc gcctgtagct gcccatcgtt tgctcgcata ggtgcataat ttagccaggg
     6721 ttttggtgct tgaacacccc acaccacagg taagggacag attatgaaga agtgtacttc
     6781 tgatcaaccg gccgtaatct tcataatcta ttatcttgtt catgtttttc aataacatga
     6841 aattttcatc tatgctatat cgactcgatt gaggcgcgaa gctttccagc gtttcatgga
     6901 agcgatttga gtagtcttca tacaaagtaa agttagagct ccggacttcg atccggtgtt
     6961 gctgaatcaa atccctgcac ttaaaaattg gggtcccacg cttaatacca acgcttttag
     7021 ccagtttatt cattgcaatg acggacgaat cgttattgct ggctatgatg agcggctttt
     7081 ttgacagctt gggatccatc gcacactcaa atgccgcata ggccccgtta atatcaacgt
     7141 gcatgtacat ggctaaactg attcagtgca aaggtgacga cgccccatat ctcaagggat
     7201 tcgggattta cacgtatcgg cttataggct ggattagaag gcatcaaaag cagctcagga
     7261 tacgtgtgta agatctttac cgtaaactct ccatccacca gcgcaacgac gatagagccg
     7321 tgtcgtgccg ttaaagaccg atccacaacc agtattgcac catcattgat accggcattg
     7381 atcatactga ggcccgccgc cctgacaaag aaggttgagg ggcggttacg gactacatat
     7441 tcatgcaaat ttaattcatt atcttcatgc ccggcgcacg gactcatcac tccacagctc
     7501 agtaaatcac tgaaaaaagg tgccatttcg taaggtttgt tgctaatgtg ctcaaggata
     7561 acagacatag ctacactcga attcttatac tgtatgtata aacagtattg cgagttacag
     7621 agaaaagatc aaatctctcc ggtaacgcta tcgagcctga cgaaaaatta agctttgtca
     7681 caaacaccaa aaaccatctt ttaccgataa tggcgagctg gcctacgaag aaggtataca
     7741 agctaggatg aaaggattct gatgcccctc ccaccaaatt ttttatctcc tgaagaccag
     7801 gctgagtacg atgcttatat gtcaagattc agcgagataa atcactatta tgattatcac
     7861 actgtccccg tgattgactg gttctttaaa caagcaaccg aggcgttgca tcacgaacta
     7921 tggataccgg cctgtacgag cttcctgaac gggattgaaa catcactcat ggttacgttg
     7981 aagtcgctga tgctgaaacc aacagttaac gatcagcatg cagctcctga gctggtggac
     8041 ttggagggta tctccgtaat gagtaatgcc ttactgagaa aggccaagca ggaaggggtg
     8101 ccggtagaat tactttcatt tccagccgag caggatatgt tggttaaggt agcagccggg
     8161 agaacacctg aagcagaaat tgtcaggctg agaaacaacc tatgccatgg caacattctg
     8221 gagtttatca tgtctgttga tatcggtact tcaggaccca tacgtatttt tacgcctgaa
     8281 tgctgtcgtg aactggcgca tgaactttcg tccatttcca ggaattgggt ggtaggtttg
     8341 cacaagtact gggtcgacaa caatctgatt tccccctgaa gctccttgaa ccttgctttc
     8401 agaagccagg tgtatgaaac cctattggat aatatttaaa gccaaaatat ggggatctcg
     8461 tattcggtga caggcatggt ataaatgatg tgtctgagca accatcattg ataatcatgg
     8521 agtgagtcat gaaattaaaa taccttctgt tatgtgcaac tctgtctgtc gcctttacct
     8581 cctctgcggt ccttgcatct gaaaacggga aaaaaccgat cccgggcaaa tcagatgtga
     8641 tggttgctaa ctatgcatct tgagggctct ttaaaggggt aagttaaaag caaagattta
     8701 cttgaaatta ttatatcatt tcgaaaatcc attacta
//
LOCUS       DAPGOF010000194         8734 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.195, whole genome shotgun sequence.
ACCESSION   DAPGOF010000194 DAPGOF010000000
VERSION     DAPGOF010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.195"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            288..854
                     /gene="mepS"
                     /locus_tag="RHV13_004077"
     CDS             288..854
                     /gene="mepS"
                     /locus_tag="RHV13_004077"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HDV1243549.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            1035..2591
                     /locus_tag="RHV13_004078"
     CDS             1035..2591
                     /locus_tag="RHV13_004078"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HDV1243550.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     KLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEVPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            2673..4487
                     /locus_tag="RHV13_004079"
     CDS             2673..4487
                     /locus_tag="RHV13_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDV1243551.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPSEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQKRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARHQGE"
     gene            4488..5582
                     /locus_tag="RHV13_004080"
     CDS             4488..5582
                     /locus_tag="RHV13_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HDV1243552.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            5582..6607
                     /locus_tag="RHV13_004081"
     CDS             5582..6607
                     /locus_tag="RHV13_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV1243553.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            6609..8198
                     /gene="yejF"
                     /locus_tag="RHV13_004082"
     CDS             6609..8198
                     /gene="yejF"
                     /locus_tag="RHV13_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HDV1243554.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAIPQQEYTRQLLALS"
     gene            complement(8202..8546)
                     /locus_tag="RHV13_004083"
     CDS             complement(8202..8546)
                     /locus_tag="RHV13_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243555.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
BASE COUNT         2062 a         2174 c         2188 g         2310 t
ORIGIN      
        1 tcactcaatc gtactttaat tgaccaatcc cgcttattaa ctttctgtat cactttttct
       61 tataaaaaat catgtaaaac cgctcgccaa gaccgcacca atcgggtaat ctcgaactcg
      121 ttttgcctcg gcggtagatt atcctcacag catataattt tgtgcgttag tccacagatt
      181 tggccttaag gaattgtttc aacatgccca ggtaattagt ctcgtgtcgc ttggcatttt
      241 tttataacga tatttgtcgt taaggacttc aagggaaaac aaacaacatg gtcaaatctc
      301 aaccgatttt gagatatatc ttgcgcggga tccccgcgat tgcagtagcg gttctgcttt
      361 ctgcatgtag tgcaaataac accgcaaaga atatgcatcc tgagacacgt gcagtgggta
      421 gtgaaacatc atcactgcaa gcttctcagg atgaatttga aaacctggtt cgtaatgtcg
      481 acgtaaaatc gcgaattatg gatcagtatg ctgactggaa aggcgtacgt tatcgtctgg
      541 gcggcagcac taaaaaaggt atcgattgtt ctggtttcgt acagcgtaca ttccgtgagc
      601 aatttggctt agaacttccg cgttcgactt acgaacagca ggaaatgggt aaatctgttt
      661 ctcgcagtaa tttgcgtacg ggtgatttag ttctgttccg tgccggttcg acgggacgcc
      721 atgtcggtat ttatatcggc aacaatcagt ttgtccatgc ttccaccagc agtggtgtta
      781 ttatttccag catgaatgaa ccgtactgga agaagcgtta caacgaagca cgccgggttc
      841 tcagccgcag ctaataaacc gtttggatgc aatcccttgg ctatcctgac gagttaactg
      901 aaagcactgc ttaggcagtg cttttttgtt ttcattcatc agagaaaatg atgtttccgc
      961 gtcttgatcc aggctatagt ccggtcattg ttatctttta aatgttgtcg taatttcagg
     1021 aaattaacgg aatcatgttc atacgcgctc ccaattctgg acgtaagctc ctgcttacct
     1081 gcattgttgc aggcgtgatg attgcgatac tggtgagttg ccttcaattt ttagtggcct
     1141 ggcataagca cgaagtcaaa tacgacaaac tgattatcga cgtacaaaag tatctcgata
     1201 cctattttgc cgacctgaaa tccactactg accggcttca gccgctgacc ttagatacct
     1261 gccagcaggc taaccccgaa ctgaccgctc gcgcggcgtt tagcatgaat gtccgtacgt
     1321 ttgtgctggt gaaagataaa aaaacattct gttcatctgc gactggtgag atggacattc
     1381 cactcaatga attgattccg gcgctcgaca ttaataaaaa cgtcgatatg gcgatcttac
     1441 ccggcacgcc gatggtgccg aacaaacccg caatcgtcat ctggtatcgc aaccctttgc
     1501 tgaaaaatag cggtgtgttt gccgctctga atctcaacct gacgccttct ctcttttata
     1561 gttcacggca ggaagattac gatggcgtcg ccctcattat tggcaatact gcgctatcta
     1621 ccttttcttc acgtttgatg aacgttaatg aattaaccga catgccggtc cgtgaaacta
     1681 aaattgcggg cattcctctg accgttcggc tttatgcgga tgactggaca tggaacgatg
     1741 tgtggtacgc atttttactg ggcggcatga gtggaactgt cgttggcctg ctctgctatt
     1801 acctgatgag cgtacgtatg cgccccggca gagaaatcat gaccgccatc aagcgcgaac
     1861 aattttacgt ggcgtatcaa ccggtggtgg atacacaagc tttgcgagta acgggcctgg
     1921 aagtactgct acgctggcgg catcctgtag cgggagaagt tcccccggat gccttcatta
     1981 actttgccga atcgcaaaag atgattgtgc cgctgactca gcacctgttt gagttgattg
     2041 cccgcgatgc cgcagaatta gaaaaagtac tgccggtagg cgtcaaattt ggcattaaca
     2101 ttgcgccgga ccatctgcac agcgaaagct ttaaagcaga tatccagaaa ctgctcactt
     2161 ccctgcccgc acaccatttc cagattgtgc tggaaattac cgagcgcgat atgctgaaag
     2221 agcaagaagc cacacaactc ttcgcctggc tgcactcggt cggcgtagaa attgctattg
     2281 atgacttcgg caccgggcac agcgcgctta tctatcttga gcgttttacg ctcgattatc
     2341 tgaaaattga ccgtggattt atcaacgcca tcggtacgga aacgatcact tcacccgtac
     2401 ttgacgcggt gctgacgctg gcgaaacgtc tcaatatgct gacagttgct gaaggggtcg
     2461 aaacgccgga acaagcgcga tggctaagcg aacgcggcgt taatttcatg caaggctact
     2521 ggattagtcg cccgttaccg ctggacgatt ttgtccgctg gctgaagaaa ccgtatacgc
     2581 cgcagtggta aggtgtgctt acgtccctta ttattcatag tgaaagcatg ccggattgcg
     2641 gctaatgatg agtaaaagga aatccgttgc agatgattgt gcgcatactg ctgctgttta
     2701 tcgctctgtt cacctttggt gcgcaggcgc aggctatcaa ggaaagctat gcctttgccg
     2761 tgctgggcga accccggtac gcgtttaatt tcaaccattt tgattatgtg aaccccgccg
     2821 cgccaaaagg tgggcagata acgttgtcag ccctcggtac cttcgataat ttcaaccgct
     2881 atgcactgcg cggcaacccg ggcgcacgca ccgagcagct gtacgacacg ctatttacga
     2941 cttccgatga cgaaccaggc agttattacc cgctgattgc tgaaagcgca cgctatgctg
     3001 acgattattc ctgggtggag gtcgctatta atccgcgcgc ccgttttcat gatggttcgc
     3061 ccattactgc ccgcgatgta gagtttactt ttcaaaaatt tatgactgaa ggcgtgccgc
     3121 aatttcgtct ggtctacaaa ggcaccaccg tcaaagccat tgcgccgtta accgtgcgca
     3181 ttgagttagc taaacccggc aaagaagata tgttgagtct gttttcgctg ccggtatttc
     3241 cagaaaagta ctggaaggat cacaaactta gcgatccgct cgccacgcct ccgcttgcca
     3301 gtggtccgta ccgaattacg tcctggaaaa tggggcaaaa tattgtctat tcccgcgtga
     3361 aagattactg ggcagcaaac ttaccggtaa accgtggacg ctggaatttc gacaccattc
     3421 gctacgatta ttacctcgat gataatgtcg cctttgaagc gtttaaagca ggtgcttttg
     3481 atttgcgtat ggaaaacgac gctaaaaact gggccacgcg ttataccggt aaaaatttcg
     3541 ataaaaaata catcatcaaa gatgagcaaa agaacgaatc agcccaggat acgcgctggc
     3601 tggcgtttaa tatccaacgt ccggtattca gcgatcgccg ggtccgggaa gcaatcaccc
     3661 tcgcctttga ctttgaatgg atgaacaagg cgttgtttta caatgcctgg agtcgcacga
     3721 acagttattt tcagaatacc gaatacgcgg ccagaaatta ccccgacgcc gcggagctgg
     3781 tgcttctggc accaatgaaa aaagatctac cgtcagaagt cttcacacaa atctaccagc
     3841 cgccggtatc taaaggcgat ggctacgatc gtgacaacct gttaaaagcc gacaaacttc
     3901 tcaacgaagc gggctgggtg ctgaagggtc agaaacgcgt taatgccaca acgggtcagc
     3961 cactcagctt tgaattattg cttcccgcaa gcagcaatag tcagtgggta ttgccgttcc
     4021 agcacagcct gcaacggctg ggtatcaaca tggacattcg caaggtggat aactcgcaaa
     4081 tcaccaaccg catgcgcagt cgcgactatg acatgatgcc gcgcctatgg cgggcgatgc
     4141 cgtggcccag ttccgattta cagatttcct ggtcatcgga atatatcaat tccacttata
     4201 atgcccccgg cgtgcaaagc ccggttatcg actcgctgat caaccaaatt attgccgcgc
     4261 agggaaataa agaaaaatta ctgccgttgg ggcgagcact ggatcgcgta ttaacgtgga
     4321 attattacat gctgccaatg tggtacatgg cggaagaccg tctcgcctgg tgggataaat
     4381 tctcccaacc cgctgtacgc cctgtttaca gcctgggtat cgatacctgg tggtatgacg
     4441 ttaataaagc ggccaaactg ccgtcagcca gacatcaggg agagtagatg ggcgcttatc
     4501 tgattcgccg tctgttgctg gtgatcccaa cgttatgggc gattatcact atcaactttt
     4561 tcatcgtgca aattgcgcct ggcggtccgg tcgaccaggc catcgccgcc attgagtttg
     4621 gtaatgccgg agtattaccc ggcgcaggcg gtgaaggcgt tcgcgccagc catgcgcaaa
     4681 cgggcgtcgg taatatcagc gacagtaatt accgtggcgg acgcggatta gatccagaag
     4741 tgatcgccga gatcactcat cgctacggtt tcgataagcc gatccacgaa cgttacttca
     4801 aaatgctctg ggactacatc cgctttgatt ttggcgatag cctgtttcgc agcgcctcgg
     4861 tgctgacgct gattaaagac agtctgccgg tttccatcac cctcggatta tggagcacgc
     4921 tgattatcta tctggtgtcg attccgttag gcattcgcaa agctgtttat aatgggagcc
     4981 gctttgacgt ctggagtagc gcatttatca tcatcggcta cgccattccg gcctttttgt
     5041 ttgccatcct gctgattgtc ttcttcgcag gcggcagcta tttcgacctg ttccctctac
     5101 gcggcctggt ttccgctaac tttgattcgc tgccgtggta tcagaaaatc accgattatc
     5161 tgtggcatat cacgctgccg gtgctggcga cggtgattgg tggctttgcg gcgctgacta
     5221 tgctgacaaa aaactcattc cttgatgaag tgcgcaagca atacgtggtg accgcgcgtg
     5281 cgaaaggggt aagtgaaaaa aatattctct ggaaacatgt gttccgcaac gccatgctgc
     5341 tggtgattgc cggttttccg gcgacgttta tcagcatgtt ttttaccgga tcgctgctga
     5401 ttgaggtgat gttttcactc aatggtcttg gcttactggg ctacgaagcg accgtctcgc
     5461 gcgattatcc tgtaatgttt ggtaccttgt atattttcac cctgattggc ctgctgctga
     5521 atattgtcag tgatatcagc tatacgctgg tcgatccgcg tatagatttt gagggacgtt
     5581 aatgtcgcga ctcagccccg tcaatcaggc ccgttgggcg cgttttcgcc ataaccgtcg
     5641 cggctactgg tcgttatgga ttttcctcgt tttgtttggt ttgagtttgt gttctgaact
     5701 tatcgccaac gataaaccgt tgctggtgcg ttatgacggc agttggtatt tcccgttgtt
     5761 gaaaaactac agcgaaagcg attttggcgg cccgctggca agtcaggctg attatcagga
     5821 cccgtggctg aaacaacggc tggaaaataa tggctgggta ctgtgggcac cgattcgctt
     5881 tggtgctacc agtatcaact ttgctaccga taagcccttc ccttctccac cctcccggca
     5941 aaactggctg ggaacggatg ccaacggcgg cgatgtgctg gcacgcattc tctatggcac
     6001 gcggatctcg gttctgtttg gcctgatgct gactctctgt tccagcgtga tgggcgtgct
     6061 ggcgggggcg ctacaaggct attacggcgg taaagtcgat ctctgggggc aacgctttat
     6121 tgaagtatgg tcggggatgc cgacgctgtt tttgattatt ttactttcca gcgtcgtaca
     6181 gcctaacttc tggtggctgc tggcaattac tgtcttgttt ggctggatga gtctggtcgg
     6241 cgtggtgcgg gcggagtttt tacgtacccg taatttcgac tacattcgtg cggcgcaggc
     6301 gcttggcgtc agcgatcgca gtatcatcct gcgtcatatg ttgccaaatg ccatggtcgc
     6361 gaccctcacc tttttaccgt ttattttatg tagttcgatt accaccctga cctcactcga
     6421 tttcctcggc ttcggtctgc cgctcggttc accgtcactc ggtgaactgc tgttacaagg
     6481 gaaaaataac cttcaggctc cgtggcttgg gatcaccgcc ttcttgtcgg tggcgatatt
     6541 attgtctttg ctgatcttta ttggtgaagc cgtccgcgac gcatttgatc ctaataaggc
     6601 ggtgtagcat gacgcaaact ctgttagcga ttgaaaattt gtcggtgggt tttcgccatc
     6661 agcaaaccgt acgtacagta gtaaatgatg tttcactaca gattgaggct ggcgaaacgc
     6721 tggcgctggt gggtgagtca ggttcaggca aaagcgttac cgcgctgtca attttacgcc
     6781 tgctcccttc cccgccggtt gaatatctct ccggcgatat tcgttttcat ggcgaatcgc
     6841 tgcttcatgc cagcgatcaa acgttgcgcg gcgtacgtgg taataagatc gccatgattt
     6901 ttcaggagcc gatggtgtcg ttaaatccat tgcataccct ggaaaaacag ctttatgaag
     6961 tgctttcact ccaccgcggg atgcgtcggg aagcggctcg tggcgaaatt cttaactgcc
     7021 ttgatcgcgt tggtatccgc caggcggcaa aacggctgac agattatcca catcagctct
     7081 ccggcggcga acgccagcgg gtgatgattg cgatggcgct gttaacgcga ccggaattat
     7141 taattgccga tgaaccgacc accgcactgg acgtctctgt ccaggcgcag attttacagc
     7201 tgttgcgcga actgcaaggc gagctgaata tgggtatgct gtttattact cataacctca
     7261 gcattgtcag aaaactggcc catcgcgtgg cggtaatgca aaacggtcgc tgtgtcgagc
     7321 aaaataacgc cgctacgcta tttgcctcac ccactcatcc ttacacacaa aagctactca
     7381 acagtgaacc gtcaggcgac ccggtaccat tgccagaacc tgcctcaacg ttgctggatg
     7441 ttgaacagct tcaggttgcc ttccccattc gcaaagggat tttgaagcgc attgtggatc
     7501 ataatgtggt ggtgaaaaac atcagtttta cgttacgggc gggtgaaaca ctgggtttag
     7561 tgggcgagtc cggttccggg aaaagcacga cgggactggc gctgctgcga ctgattaatt
     7621 ctcagggcag catcatcttt gacggtcagc cactgcaaaa tttaaatcgc cgccagctgt
     7681 tacctattcg tcatcgcatt caggtggtat ttcaggatcc aaactcctcg ctcaacccac
     7741 gactcaacgt tttgcagatt attgaggaag gcttacgggt tcaccagccg acgctatctg
     7801 ccgcacaacg cgaacaacaa gtgatagccg tgatgcatga agtgggatta gatcctgaaa
     7861 cacgccaccg ttatccggcg gagttctctg gtggtcagcg acaacgtatt gcgattgcca
     7921 gggcattaat tcttaagccc tcgctgatca tacttgatga accgacatca tcactcgaca
     7981 aaacggtaca ggcgcaaata ttgacgctat tgaaatcatt gcaacaaaag catcaactgg
     8041 cctatttgtt tatcagccat gatttgcacg ttgtccgcgc gttatgtcat caggttatcg
     8101 tactgcgaca aggggaagta gtggaacaag gaccgtgcgc gcgcgtgttt gccataccgc
     8161 agcaggagta tacgcgtcag ctactggcgt tgagctgacg cttaaaaagg attgtagttt
     8221 gaaaagggtt cggcaattgc cacaccaaaa tttttcaatc ggcatgttgc agcaaactcg
     8281 tcctggcgat ttacaaacag gcacggctca ccttcacact ccagcaccga acatggaact
     8341 tcgatgtcgc ttaacgcctg gcttagttta tacatcaccg accatgcatt atcaccatcc
     8401 gggctaagca atttaagcgc caccaggctg ttgtcaccgc acggtccatt ttgcggaatc
     8461 ggttcaacct tcgaacctgc gaaaccagca gaccagcgat agttctgcgg cagtcgatgg
     8521 acgattgaga gttgtaatga agtcacttat tttccccgga agcacattta cttcacaatt
     8581 tgtttacatc aattttaaca catcatcaac aatccgtctt tatacagatc gtaaagattg
     8641 atgcctcgcc tgcgtggcct ctatggctct gatttaagta atatctgcgg gcgtggtcac
     8701 gtttgcgggg ctatatgtac ccgtttctgc gatc
//
LOCUS       DAPGOF010000195         8627 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.196, whole genome shotgun sequence.
ACCESSION   DAPGOF010000195 DAPGOF010000000
VERSION     DAPGOF010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8627
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.196"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(286..549)
                     /gene="rpsT"
                     /locus_tag="RHV13_004084"
     CDS             complement(286..549)
                     /gene="rpsT"
                     /locus_tag="RHV13_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDV1243556.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            652..870
                     /locus_tag="RHV13_004085"
     CDS             652..870
                     /locus_tag="RHV13_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HDV1243557.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            878..1819
                     /gene="ribF"
                     /locus_tag="RHV13_004086"
     CDS             878..1819
                     /gene="ribF"
                     /locus_tag="RHV13_004086"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HDV1243558.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGHHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1862..4678
                     /gene="ileS"
                     /locus_tag="RHV13_004087"
     CDS             1862..4678
                     /gene="ileS"
                     /locus_tag="RHV13_004087"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDV1243559.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4678..5172
                     /gene="lspA"
                     /locus_tag="RHV13_004088"
     CDS             4678..5172
                     /gene="lspA"
                     /locus_tag="RHV13_004088"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDV1243560.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5260..5709
                     /gene="fkpB"
                     /locus_tag="RHV13_004089"
     CDS             5260..5709
                     /gene="fkpB"
                     /locus_tag="RHV13_004089"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDV1243561.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5711..6661
                     /gene="ispH"
                     /locus_tag="RHV13_004090"
                     /gene_synonym="lytB"
     CDS             5711..6661
                     /gene="ispH"
                     /locus_tag="RHV13_004090"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HDV1243562.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6727..7641
                     /gene="rihC"
                     /locus_tag="RHV13_004091"
     CDS             6727..7641
                     /gene="rihC"
                     /locus_tag="RHV13_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HDV1243563.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            7808..>8627
                     /gene="dapB"
                     /locus_tag="RHV13_004092"
     CDS             7808..>8627
                     /gene="dapB"
                     /locus_tag="RHV13_004092"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDV1243564.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIESHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKENGLFDMRDVLDLNNL"
BASE COUNT         1977 a         2156 c         2444 g         2050 t
ORIGIN      
        1 atttaattca aaaatagagt ggtcttaagt ttaatacctc aatgtgtatc acaatatcca
       61 cattctttgt aggggagtct ggagattgag tagatattct tgttcagaat gtatcagccg
      121 atggttctac gattcttaag ccacgaagag ttcagatagt acaacggcat gtctcttttg
      181 actatctggc aaccggcagt gtgttctctc acgcatcaca aaagcagcag gcataaaaaa
      241 acccgcttgc gcgggctttt tcacaaagct tcagcaaatt ggcgattaag ccagtttgtt
      301 gatctgtgca gtcaggttag ccttatgacg tgcagctttg tttttgtgga tcagaccttt
      361 agcagcctga cggtccacga tcggttgcat ttcgttaaat gctttctgtg cagcagcttt
      421 gtcgccagct tcgatagctg cgtatacttt cttgatgaaa gtacgcatca tagagcgacg
      481 gcttgcgttg tgcttacgag ccttttcaga ctgaatggcg cgcttcttag ctgatttgat
      541 attagccaag gtccaactcc caaatgtgtt ctatatggac aattcaaagg ccgaggaata
      601 tgccctttta gccttctttt gtcaatggat ttgtgcaaat aagcgccgtt aatgtgccgg
      661 cactcgttac gtagtgatgg cgcaggattc taccagcttg cggggtgtga atacagcttt
      721 tccgcgataa aaattgcagc aggcggtcag tttcttcccg tgatttgcgc catggcaatg
      781 aaaagccact tctttctgat ttcggtactc aatcgccggt taaccttgac cgctgtacaa
      841 ggtatactcg gacgattttc actgttttga gccagacatg aagctgatac gcggcataca
      901 taatctcagc caggccccgc aagaagggtg tgtgctgact attggtaatt tcgacggcgt
      961 gcatcgcggt catcgcgcgc tgttacaggg cttgcaggaa gaagggcgca agcgcaactt
     1021 accggtgatg gtgatgcttt ttgaacctca accactggaa ctatttgcta ccgataaagc
     1081 cccggcaaga ctaacccggc tgcgggaaaa actacgttac cttgcagagt gtggcgttga
     1141 ttacgtgctg tgcgtacgtt tcgacaggcg tttcgcggcg ttaaccgcgc aaaatttcat
     1201 cagcgatctt ctggtgaagc atttgcgcgt aaaatttctt gccgtaggtg atgatttccg
     1261 ctttggcgct ggtcgtgaag gcgatttctt gttattacag aaagctggca tggaatacgg
     1321 cttcgatatc accagtacgc aaactttttg cgaaggtggc gtgcgtatca gcagcaccgc
     1381 cgtgcgtcag gcgctggcgg atgacaatct ggctctggca gaaagtttac tggggcaccc
     1441 gtttgccatc tccgggcgtg tagtccacgg tgatgaatta gggcgcacta taggtttccc
     1501 gacggcgaat gtaccgctac gccgtcaggt ttccccggtg aaaggggttt atgcggtaga
     1561 agtgctgggc ctcggtgaaa agccgttacc cggcgtggca aacatcggaa cacgcccaac
     1621 ggttgccggt attcgccagc agctggaagt gcatttgtta gatgttgcaa tggaccttta
     1681 cggtcaccat atacaagtag tgctgcgtaa aaaaatacgc aatgagcagc gatttgcgtc
     1741 gctggacgaa ctgaaagcgc agattgcgcg tgatgaatta accgcccgcg aattttttgg
     1801 gctaacaaaa ccggcttaag cctgttatgt aatcaaaccg aaacacggaa ccgagaatct
     1861 gatgagtgac tataaatcaa ccctgaattt gccggaaaca gggttcccga tgcgtggcga
     1921 tctcgccaag cgcgaaccgg gaatgctggc gcgttggact gatgatgatc tgtacggcat
     1981 catccgtgcg gctaaaaaag gcaaaaaaac cttcattctg catgatggcc ctccttatgc
     2041 gaatggcagc attcatattg gtcactcggt taacaagatt ctgaaagaca ttatcgtgaa
     2101 gtccaaaggg ctttccggtt atgactcgcc gtatgtgcct ggctgggact gccacggtct
     2161 gccgatcgag ctgaaagtag agcaagaata cggtaagccg ggtgagaaat tcaccgccgc
     2221 cgagttccgc gccaagtgcc gcgaatacgc ggcgacccag gttgacggtc aacgcaaaga
     2281 ctttatccgt ctgggcgtgc tgggcgattg gtcgcacccg tacctgacca tggacttcaa
     2341 aactgaagcc aatatcatcc gcgcgctggg taaaatcatc ggcaatggtc acctgcacaa
     2401 aggcgcgaag ccggtgcact ggtgcgtaga ctgccgttct gcactggcag aagcggaagt
     2461 tgagtattac gacaaaactt ctccgtccat tgacgtcgct ttccaggcgg tcgatcagga
     2521 tgcgctgaaa gcgaaatttg gcgtaagcaa cgttaacggc ccaatctcgc tggtgatctg
     2581 gaccactacg ccgtggactc tgcctgcgaa ccgcgcaatc tctattgcac ctgatttcga
     2641 ctatgcgctg gtgcagatcg acggtcaggc cgtgattctg gcgaaagatc tggttgaaag
     2701 cgtaatgcag cgtatcggcg tgaccgatta caccattctc ggcacggtaa aaggtgcaga
     2761 gcttgagctg ctgcgcttta cccatccgtt tatgggcttc gacgttccgg caatcctcgg
     2821 cgatcacgtt accctggatg ccggtaccgg tgccgttcac accgcgcctg gccacggccc
     2881 ggacgactat gtgatcggtc agaaatacgg cctggaaacc gctaacccgg ttggcccgga
     2941 cggcacttat ctgccgggca cttatccgac gctggatggt gtgaacgtct tcaaagcgaa
     3001 cgacatcgtc gttgcgctgc tgcaggaaaa aggcgcgctg ctgcacgttg agaaaatgca
     3061 gcacagctat ccgtgctgct ggcgtcacaa aacgccgatc atcttccgcg cgacaccgca
     3121 gtggttcgtc agcatggatc agaaaggtct gcgtgcgcag tcactgaaag agatcaaagg
     3181 cgtgcagtgg atcccggact ggggccaggc gcgtatcgag tcgatggttg ctaaccgtcc
     3241 tgactggtgt atctcccgtc agcgcacctg gggcgtaccg atgtcactgt tcgtgcacaa
     3301 agacacggaa gagctgcatc cgcgtaccct cgaactaatg gaagaagtgg caaaacgcgt
     3361 tgaagttgac ggcatccagg cgtggtggga tcttgatgcg aaagagatcc tcggcgatga
     3421 agctgatcag tacgtgaaag tgccggacac attggatgta tggtttgact ccggatctac
     3481 ccactcttct gttgttgacg tgcgtccgga atttgccggt cacgcagcgg acatgtatct
     3541 ggaaggttct gaccagcacc gtggttggtt catgtcttcc ctgatgatct ccaccgcgat
     3601 gaaaggcaaa gcgccgtatc gtcaggtact gacccacggc tttaccgtgg atggtcaggg
     3661 tcgcaagatg tctaaatcca tcggcaacac cgtttcgccg caggatgtga tgaacaaact
     3721 gggcgcggat attctgcgtc tgtgggtggc atcaaccgac tacaccggtg aaatggccgt
     3781 ttctgacgag atcctgaaac gtgctgccga tagctatcgt cgtatccgta acaccgcgcg
     3841 cttcctgctg gcaaacctga acggttttga tccagcaaaa gatatggtga aaccggaaga
     3901 gatggtggta ctggatcgct gggccgtagg ttgtgcgaaa gcggcacagg aagacatcct
     3961 caaggcgtac gaagcatacg atttccacga agtggtacag cgtctgatgc gcttctgctc
     4021 cgttgagatg ggttccttct acctcgacat catcaaagac cgtcagtaca ccgccaaagc
     4081 ggatagcgtg gcgcgtcgta gctgccagac tgcgctgtat cacatcgctg aagcgctggt
     4141 gcgctggatg gcaccaatcc tctccttcac cgctgatgaa gtgtggggct acctgccggg
     4201 cgaacgtgaa aaatacgtct tcaccggtga gtggtacgaa ggcctgtttg gtctggcaga
     4261 cagtgaagca atgaacgatg cgttctggga cgagctgttg aaagtgcgtg gcgaagtgaa
     4321 caaagtcatt gagcaagcgc gtgccgataa gaaagtgggt ggctcgctgg aagcggcagt
     4381 aaccttgtat gcagaaccgg aactggcggc gaaactgacc gcgctgggcg atgaattacg
     4441 atttgtcctg ttgacctccg gcgctaccgt tgcagactat aatgacgcac ctgctgatgc
     4501 tcagcagagc gaagtactca aagggctgaa agtcgcgttg agtaaagccg aaggtgagaa
     4561 gtgcccacgc tgctggcact acacccagga tgtcggcaag gtggcggaac acgcagaaat
     4621 ctgcggccgc tgtgtcagca acgtcgccgg tgacggtgaa aaacgtaagt ttgcctgatg
     4681 agtcaatcga tctgttcaac agggctacgc tggctgtggc tggtggtagt cgtgctgatt
     4741 atcgatctgg gcagcaaata cctgatcctc cagaactttg ctctggggga tacggtcccg
     4801 ctgttcccgt cgcttaatct gcattatgcg cgtaactatg gcgcggcgtt tagtttcctt
     4861 gccgatagcg gcggctggca gcgttggttc tttgccggta ttgcgattgg tattagcgtg
     4921 atcctggcag tgatgatgta tcgctcgaag gccacgcaga agctaaacaa tatcgcttat
     4981 gcgctgatta ttggcggcgc gctgggcaac ctgttcgacc gcctgtggca cggcttcgtt
     5041 gtcgatatga tcgacttcta cgtcggcgac tggcacttcg ccaccttcaa ccttgccgat
     5101 actgccattt gtgtcggtgc ggcactgatt gtgctggaag gttttttacc ttctaaagcg
     5161 aaaaaacaat aataaaccct gccggataag gcctacagat tgctgcgaaa tcgtaggccg
     5221 catccggcaa aaatcctcaa aatataagag caaacctgca tgtctgaatc tgtacagagc
     5281 aatagcgccg tcctggtgca cttcacgcta aaactcgacg atggcaccac cgccgagtct
     5341 acccgcaaca acggtaaacc ggcgctgttc cgcctgggcg atgcttctct ttctgaaggg
     5401 ctggagcaac acctgctggg gctgaaagtg ggcgataaaa ccaccttctc gctggagccc
     5461 gatgcggcgt ttggcgtgcc gtcaccggac ctgattcagt acttctcccg ccgtgaattt
     5521 atggatgcag gcgagccaga aattggcgca attatgcttt ttaccgcaat ggatggcagt
     5581 gagatgcctg gcgtgatccg cgaaattaac ggcgactcca ttaccgttga tttcaaccat
     5641 ccgctggccg ggcagaccgt tcattttgat attgaagtgc tggaaatcga tccggcactg
     5701 gaggcgtaac atgcagatcc tgttggccaa cccgcgtggt ttttgtgccg gggtagaccg
     5761 cgctatcagc attgttgaaa acgcgctggc catttacggc gcaccgatat atgtccgtca
     5821 cgaagtggta cataaccgct atgtggtcga tagcttgcgt gagcgtgggg ctatctttat
     5881 tgagcagatt agcgaagtac cggacggcgc gatcctgatt ttctccgcac acggtgtttc
     5941 tcaggcggtt cgtaacgaag cgaaaagccg cgatttgacg gtattcgatg ccacctgtcc
     6001 gctggtgacc aaagtgcata tggaagtcgc ccgcgccagc cgtcgtggcg aagaatctat
     6061 tctcatcggc cacgccgggc acccggaagt ggaagggacg atggggcagt acagcaaccc
     6121 ggaaggggga atgtatctgg tcgaatcgcc agacgatgtg tggaaactga cggtcaaaaa
     6181 cgaagagaag ctctccttta tgacccaaac cacgctgtcg gtggatgaca cgtctgatgt
     6241 gatcgacgcg ctgcgtaaac gcttcccgaa aattgtcggt ccgcgcaaag atgacatctg
     6301 ctacgccacg actaaccgac aggaagcggt acgtgccctg gcagaacagg cggaagttgt
     6361 gctggtggtc ggttcgaaaa actcctctaa ctccaaccgt ctggcggagc tggcccagcg
     6421 tatgggcaaa cgcgcgtttt tgattgatga tgcgaaagat atccaggaag agtgggtgaa
     6481 agaggttaaa tgcgtcggcg tgactgcggg cgcatcagct ccggatattc tggtgcagaa
     6541 tgtggtggca cgtttgcaac agctgggcgg tggtgaagcc attccgctgg aaggacgtga
     6601 agaaaacatt gttttcgaag tgccgaaaga gctgcgtgtc gatattcgtg aagtcgatta
     6661 agtcattagc agcctaagtt atgcgaaaat gccggtcttg ttaccggcat tttttatgga
     6721 gaaaacatgc gtttacctat cttcctcgat actgaccccg gcattgacga tgccgtcgcc
     6781 attgccgccg cgatttttgc ccccgaactc gacctgcaac tgatgaccac cgtcgcgggt
     6841 aatgtctcgg ttgagaaaac cacccgcaac gccctgcaac tgctgcattt ctggaatgcg
     6901 gagattccgc tcgcccaggg agccgctgtg ccgctggtac gcgcaccgcg tgatgcagca
     6961 tctgtgcacg gcgaatcggg aatggctggc tacgactttg ttgagcacaa ccgaaagcca
     7021 ctcgggatac cggcgtttct ggcgattcgg gatgccctga tgcgtgcacc agagcctgtt
     7081 accctggtgg ccatcggccc gttaaccaat attgcgctgt tactttcaca atgcccggaa
     7141 tgtaaaccgt atattcgtcg tctggtgatc atgggcggtt ctgccggtcg cggcaactgc
     7201 acgccaaacg ccgagtttaa tatcgctgcc gatccagaag cggcagcctg tgtcttccgt
     7261 agcggcatcg aaatcgtcat gtgcggtctg gatgtcacca atcaggcaat attaacccct
     7321 gactatctcg ctactttgcc ggagttgaat cgcaccggaa aaatgctcca cgccctgttt
     7381 agccactacc gtagcggcag tatgcaaagc ggtctgcgaa tgcacgatct ctgcgccatc
     7441 gcctggctgg tgcgcccgga cttattcacc ctcaagccgt gttttgtcgc ggtagaaact
     7501 caaggcgaat ttacctcagg cacgacggtg gttgatatcg acggttgcct gggcaaacca
     7561 gccaatgtac aggtggcatt ggatctgaat gtaaaaggct tccaacagtg ggtggctgag
     7621 gtgctggctc tggtgccgta acctgtcaca tgttatcggc atgcagtcat tcatcgactc
     7681 atgcctttca ctgatatccc tccctgttta tcattaattt ctaattatca gcgtttttgg
     7741 ctggcggcgt agcgatgcgc tggttactct gaaaacggtc tatgcaaatt aacaaaagag
     7801 aatagctatg catgatgcaa acatccgcgt tgccatcgcg ggagccgggg ggcgtatggg
     7861 ccgccagttg attcaggcgg cgctggcatt agaaggcgtg cagctgggcg cggcgctgga
     7921 gcgcgaagga tcttctttac tgggcagcga cgccggtgag ctggcgggtg cagggaaaac
     7981 aggcgttacc gtgcaaagca gtcttgatgc gataaaagat gattttgatg tgtttatcga
     8041 ttttacccgt ccggaaggta cgctgaacca tctcgctttt tgtcgccagc atggcaaagg
     8101 gatggtgatc ggcactacgg ggtttgacga agccggtaaa caggcaattc gtgacgccgc
     8161 cgccgatatt gcgattgtct ttgccgccaa ttttagcgtt ggcgttaacg tcatgcttaa
     8221 gctgctggag aaagcagcca aagtgatggg tgactacacc gatatcgaaa ttattgaatc
     8281 acatcataga cataaagtgg atgcgccgtc aggcaccgca ctagccatgg gagaggcgat
     8341 cgcccacgcc cttgataaag atctgaaaga ttgcgcggtc tacagtcgtg aaggccatac
     8401 cggtgaacgt gtgcctggta ccattggttt tgctactgtg cgtgcaggtg acatcgttgg
     8461 cgaacatacc gcgatgtttg ccgatattgg cgagcgtctg gagatcaccc ataaggcgtc
     8521 cagccgcatg acatttgcta acggtgcggt aagatcggct ttgtggttga gtggtaagga
     8581 aaatggtctt tttgatatgc gagatgtact tgatctcaat aatttgt
//
LOCUS       DAPGOF010000196         8330 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.197, whole genome shotgun sequence.
ACCESSION   DAPGOF010000196 DAPGOF010000000
VERSION     DAPGOF010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.197"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..4015)
                     /gene="air"
                     /locus_tag="RHV13_004093"
     CDS             complement(<1..4015)
                     /gene="air"
                     /locus_tag="RHV13_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin EaeX/Air"
                     /protein_id="HDV1243565.1"
                     /translation="MAGKAHGNGDRRGDNTICGLGDRLRRLTAGICLITQTIFPVMAA
                     APTHINHAHSDTATSLILPNVKTIPYTLGALESPSTVAARFGITVDELRRLNQFRTFA
                     RGFDNVRQGDEIDVPLINSNSLEARNLKAMQMERDGKDPQMQVAEVAQQSGTLLARDM
                     DSEQAASMARGWVASSASAQATDWLSRWGTARVSLGVDEDFSLKSSSFEFLHPWYETP
                     DNLVFSQHTLHRTDDRTQTNHGIGWRYFTPSWMSGVNMFIDHDLTRYHTRTGMGVEYW
                     RDYLKLSGNGYLRLSNWRSAPELDNDYEARPANGWDLRAEGWLPAWPQLGGKLVYEQY
                     YGDEVALFGKDERQNDPHAITAGLSYTPVPLISFSAEQRQGKQGENDTRIGMELTLQP
                     GHSLQKQLDPAEVAARRSLVGSRYDLVDRNNNIVLEYRKKELVRLTLTDPLKGKPGEV
                     KSLVSSLQTKYALKGYDIEAAALQSAGGKVAVSGKDIQVTIPPYRFTAMPETDNTYPI
                     AVTAEDSKGNFSRREESMVVVEKSTLSLADSTLSVDLQILLADGKSTSTLTYTARDSS
                     GKPIPGMTLKTQAKGLQDFALSEWKDNGNGTYTQIVTAGKTSGALSLMPQFNGDDIAK
                     TPALIAIVANTASRADSTIETDQDNYVAGKPIVVKVTLRDDNGNGVTGRKELLKQTVK
                     VDNTKADAVSAWTEESEGIYKASYTAHLIGDKLTAQLTMPGWQTKHSDAFSIAGDKDT
                     AKIAAMQITANNAVARRDHNTVAVTVRDVHQNLLQGQNVTFTVVNGAAVFADPNGGIV
                     TTDKDGIASVNLASDQAVNSLIKAEINGSSQSVEVSFITGDISQLTSTIKTDDVSYTA
                     GGKIKVSVTLMDEQKNQVKGMASLLAGSGVVEVSGTDKNETGNWSEESDGVYTTTRTA
                     KIAGDRHYATLKLSTWSSAQQSDAYAIRESGAVLAYSSIVTDKTTYTAGGAIKVTVTL
                     KDSYENLVGGQRDAINLAIQLPNTKAESIAWNEDQKGIYTATYTALLPGTGLKAQLQM
                     SGWANALTSNDYSISGDAASAQIVAMQVTTGNPDVLANGSDRHTVNVRVEDQFGNVLP
                     EQTVTFTVTKGAAVFANAGQSADIRTDAHGMAEVDLSSTVADASTVEAKVNQSSDSKT
                     VNFVADVSTAQIADLVVIKDGSVADGSTANTLRVRVTDAFGNALGGQTVSVLADNSAT
                     VAPTVITEPDGTVEISVTSQTAGVSAVTASINNSSLSRNVTFVADVRTAKIADLVVMQ
                     DGSVADGTTANTLRARVTDAFGNALAGQTVSVTAGNSATVASTVTTKPDGTVEISVTS
                     QTAGISTVTATINSSSQS"
     gene            complement(4217..5401)
                     /gene="nepI"
                     /locus_tag="RHV13_004094"
     CDS             complement(4217..5401)
                     /gene="nepI"
                     /locus_tag="RHV13_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HDV1243566.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFSVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MK"
     gene            5635..5988
                     /locus_tag="RHV13_004095"
     CDS             5635..5988
                     /locus_tag="RHV13_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDV1243567.1"
                     /translation="MFKTKWFAREARSHAITDEELCRAILETEQGKADALGGGVFKKR
                     LHQNRERAIILAKGGSNWFYTFLYAKQDMSNINSQELAGFRELAKHYAFLTKAQLTAM
                     INTKELTEICYDCKN"
     gene            5972..6295
                     /locus_tag="RHV13_004096"
     CDS             5972..6295
                     /locus_tag="RHV13_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HDV1243568.1"
                     /translation="MTAKTKFKSPAFEAIHSAAAGLSSVDAISAETMRTFDKACLTSV
                     QDLQPVEIKALREKLKVSQPVFAHYLNTSVSTVQKWESGAKRPNGISLKLLSIVQKHG
                     LDVLL"
     gene            complement(6411..6863)
                     /locus_tag="RHV13_004097"
     CDS             complement(6411..6863)
                     /locus_tag="RHV13_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HDV1243569.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(6916..8250)
                     /gene="adeQ"
                     /locus_tag="RHV13_004098"
                     /gene_synonym="yicO"
     CDS             complement(6916..8250)
                     /gene="adeQ"
                     /locus_tag="RHV13_004098"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HDV1243570.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGIMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
BASE COUNT         2131 a         2262 c         1989 g         1948 t
ORIGIN      
        1 ggctctgact gctgctgttg atggttgcag tgactgtact gatccccgcc gtctggctgg
       61 tgacactgat ctccaccgtg ccgtccggct ttgtggtcac tgtcgaagcg accgttgcgc
      121 tgttgcctgc agtcacgctg accgtctgcc cggcaagcgc attgccgaac gcatcggtca
      181 ccctcgcccg cagcgtgttc gccgtcgtgc catctgccac tgagccgtcc tgcatcacca
      241 ccagatcagc gatcttcgcc gtcctgacat cggcaacaaa cgtcacgttc cggctcaggc
      301 tgctgttgtt gatactggcg gtgaccgcac tgactcccgc cgtctggctg gtaacagaga
      361 tctccaccgt cccgtccggc tcggtgataa ccgtcggagc gaccgttgcg ctgttgtccg
      421 ccaacacgct gaccgtctgc ccgcccagcg cattaccgaa cgcatccgtc accctcaccc
      481 gcagcgtgtt cgccgtcgaa ccgtctgcca ctgagccatc cttaatgacc accagatcag
      541 cgatctgcgc cgtgctgaca tcagcaacaa agttcaccgt tttactatcg ctgctttgat
      601 tgaccttagc ctcaacagtg ctggcatccg ctacggtact gctcagatca acctctgcca
      661 taccatgcgc atccgtcctg atatctgcgc tctgccctgc attggcaaat acagccgccc
      721 ccttcgtcac cgtaaaggtg acggtctgct ccggcagcac attgccaaac tgatcttcca
      781 cccggacatt caccgtatgt cgatcgctgc catttgccag cacatccggg ttacccgttg
      841 tcacttgcat cgcgacaatt tgtgcgctgg cggcatcgcc actaatactg taatcatttg
      901 aagtcagagc gttcgcccag ccggacatct gtagctgtgc ttttaaaccg gtgccaggga
      961 gtaaggcggt atacgttgcg gtataaattc ctttttgatc ttcattccag gcgatgcttt
     1021 ccgcttttgt atttggtagc tggatagcca ggttgatagc gtctcgttgc cccccgacca
     1081 ggttttcata gctatccttg agtgtcaccg taacttttat cgcaccgccc gcggtgtagg
     1141 tcgttttgtc ggtaacgatg gaggaataag ccagcacagc tccgctttcg cggatggcat
     1201 acgcatcgga ttgctgcgcg ctactccatg tcgatagttt cagcgtggcg taatggcgat
     1261 cgccagcaat cttcgcggtt cgggtggtgg tataaacgcc gtcagactct tcgctccagt
     1321 tacccgtttc atttttatct gtaccgctca cctcgaccac accgctaccc gcgagcagcg
     1381 aagccatccc tttcacctgg tttttctgct catccataag ggtgacggac acttttatct
     1441 tgccacccgc agtgtatgac acatcgtccg tcttgatggt ggaggtcagc tgcgagatat
     1501 cgccagtgat aaagctcact tccacagact gactactgcc attgatctcg gccttgataa
     1561 gactgtttac cgcctggtca ctggcaaggt taacgctggc aattccgtct ttatctgtcg
     1621 tcacaatacc gccgtttgga tcggcaaaaa ccgccgcacc attcacgacc gtgaaggtaa
     1681 cattttgtcc ctgcagtaaa ttttgatgca catccctgac cgtcacagcg acggtattgt
     1741 gatcgcgtct ggcaactgcg ttattcgcgg tgatctgcat cgcagcgatt ttggcggtat
     1801 ctttatcacc cgcaatactg aacgcatccg aatgtttggt ttgccagccg ggcatcgtta
     1861 gctgggccgt taatttgtcg ccaatgagat gggccgtata gcttgctttg taaattccct
     1921 cactttcttc cgtccaggca ctaacagcat cagccttcgt gttgtctact ttaacggttt
     1981 gtttgagaag ttcttttctt ccggtaaccc cgttgccgtt atcatctctc agggtcactt
     2041 taacgactat cggcttaccc gcgacgtaat tatcctggtc tgtctcaatc gtggagtcag
     2101 cacgggatgc cgtattcgct acaatggcaa tcagcgctgg cgtcttagcg atatcgtcgc
     2161 cgttaaattg cggcatcagc gataacgcgc cagacgtctt cccggcggta actatttggg
     2221 tataggttcc gttaccgttg tctttccatt cgcttaaggc gaaatcctgc aaaccttttg
     2281 cctgtgtttt taacgtcata ccggggatcg gcttgccgct ggaatctcgc gcggtatagg
     2341 tcagcgttga ggttgatttt ccatcagcaa gcaggatttg cagatcgaca gaaagcgttg
     2401 aatcggcgag gcttagtgtg gatttttcca cgaccaccat actttcttca cgccgggaga
     2461 aatttccttt cgaatcttcc gctgtaaccg ctatcgggta agtgttgtcg gtttcaggca
     2521 ttgccgtgaa tcggtaagga ggaatagtga cctgaatatc cttgccggaa actgctactt
     2581 ttccgcccgc tgattgcagg gcggcggctt caatgtcata acctttcagc gcatatttgg
     2641 tctgtaagga agaaaccagc gatttaacct cacccggctt gcctttcagc ggatcggtca
     2701 gcgtcagccg taccagctct ttcttgcgat attcgaggac gatattgtta ttacgatcga
     2761 ccaggtcata gcgactgccc acaaggctgc gccgagctgc aacttcagct ggatcgagct
     2821 gtttttgcag tgaatgacca ggttgcaacg tcaactccat accaattcgg gtgtcgtttt
     2881 ctccctgttt gccctgccgt tgctcggcac taaaggagat cagaggaacg ggggtgtaac
     2941 tcaaacctgc ggtaatcgcg tgaggatcgt tttgtcgctc gtctttacca aacagcgcca
     3001 cttcatcgcc ataatattgt tcatagacca gcttgccgcc cagctgcggc caggccggta
     3061 accagccttc agcgcgaaga tcccaaccgt tcgccggacg tgcttcatag tcattgtcca
     3121 gttccggtgc actacgccag ttactgaggc gcagataacc gttgccgctc agtttcaggt
     3181 aatcgcgcca gtattcaacg cccatcccgg ttcgggtgtg atagcgggtc aggtcatgat
     3241 cgataaacat attgacgcca gacatccagc tcggggtgaa gtaacgccag ccgataccgt
     3301 ggttggtttg agtgcgatca tccgtacggt gaagtgtgtg ctggctgaag accaggttat
     3361 ccggcgtttc gtaccaggga tggagaaatt caaatgacga gcttttaagg ctgaaatctt
     3421 catccacacc aagagatacc cgggcagttc cccaacgact gagccagtcc gttgcctgcg
     3481 cagatgcaga agaagccacc cagccacgcg ccatactcgc cgcctgttcg ctgtccatat
     3541 cgcgagctaa aagtgttccg ctttgctgcg ccacttccgc gacctgcatt tgcggatctt
     3601 tgccgtcgcg ctccatctgc attgctttca agttgcgcgc ttcgaggcta ttactgttaa
     3661 tgaggggcac atctatttca tcgccctgac gcacattgtc aaagccccgc gcaaaggtac
     3721 gaaactgatt caggcgacgt aattcatcaa cggtgatacc aaagcgtgcc gcgaccgttg
     3781 atggcgattc caatgccccc agggtatatg gaatagtttt aacgttcggc aggatcagtg
     3841 acgtggcagt atccgaatgc gcatggttaa tatgcgtagg ggcagcggcc ataacaggga
     3901 atatagtttg tgttatcagg caaataccgg cggtcagacg acgtaaacga tcccccaatc
     3961 cacatatggt gttatcgccc cgacgatcgc cgttcccatg cgctttccct gccatttgtc
     4021 atccctgtcg ctatgcgttc agattaaatg acctttcttt aacatgaggg aaaatgatga
     4081 ttcatgatta gcaaatgatg aatacaggcg gaataatgga aataacaaca agataagaac
     4141 aatacctgat caagggttat cagggggcag aacaggagag ctttcccgcc agagcaataa
     4201 ccctcctgta aatacgttac ttcattttca cctttgcggt gaccaataac gccgtcagca
     4261 acatcaatgt gccggacaac atcaacggcg aagtcagacc aatattatcc agcgcataac
     4321 cgccgattgc cgcgccacag gtattagcaa gctgaataac cgccacctga atagacccgg
     4381 ctttttctgc ctgatcggcc agtgagcggg tgatccacgt tgaccagccg acaggaacca
     4441 atgcaaaagt tagcccccag ataatcgcca cgccggtcgc aacgatttta tcgcttcccc
     4501 acaacgtcag caccaacgca ctcacagcca gtattaacgg cgcgcctgct aaggccagtt
     4561 ttaccgaacg tttaagaatg aacgacgaaa gcgacgtacc aataaagctg gcaatgccaa
     4621 aactcaacag caccagcgtt aagccatcca cgctgaatcc cgccaggttc atatacaccg
     4681 ggcgaatata cgtgaagaaa gcaaactgcc cggcgaaaga catgaagatg gcgatcatcc
     4741 ctgccatcac acccggacgt tgtaataagc ggaaagtatt ttgtttctga tgcgagggtt
     4801 cgcctggcag cgaaggcaat gatttgataa tccagaaaat acacaacacg cccatcaccg
     4861 ccgccgcatt aaagacattg cgccaaccga taagctcgcc taaaaaactg cccaacggcg
     4921 cggcaatcac cagcgcaata gaaaccgcac cgaagatcac cgacagcgcc ttcggcaccg
     4981 tacgcggcgg caccagacgc atggtcagcg acgccgacat cgcccagaac ccgcccagcg
     5041 ccagccccag acaggcacga ccaattaaaa gcaaactgaa tgagttagca aaggaaacca
     5101 gcaaacagga gagcgtcagc aaaacggcaa acaaaataac aacgtagcgg cggtcagtgg
     5161 cctgaattgt ctgggtaata aacaaactgg caaacattgc cacaaaggcg gtcacggtca
     5221 ccgattgccc ggcaacccct tccgaaatgc ctaaatcctg ggccattggc gtcaacaaac
     5281 tgacgggcaa aaactcaacg ataatcagac aggcgacgca aaacgccacc gagaaaacgg
     5341 ctgaccagtt cggtcgggtg atggcatccg cgccgcggtt ttcggcaata aattcactca
     5401 tggtgttacc cgtggcatgt ttcaggggaa aagccgacca ctttaacatt gaaatgtgtg
     5461 atgcatttaa cgtttttgca acttttacgg tgttgtggga aaagttcgag gctgctcgaa
     5521 gaaataaaaa tcatagggca agctgatatg cattttcgcg attgggttgg gaataaggga
     5581 taaagtattg cacttggtga tatactttaa taataggacc tcaacgatat gcgcgtgttt
     5641 aaaaccaaat ggtttgcaag ggaagccagg tctcatgcaa taacagatga agagttgtgc
     5701 cgggccattt tagaaacgga gcaaggtaaa gctgatgcac ttggtggtgg cgtttttaaa
     5761 aagcgccttc accaaaatcg tgagcgtgca attattctgg caaaaggggg aagcaattgg
     5821 ttttacacgt ttttatatgc aaaacaggat atgtcgaaca tcaatagtca ggaactggct
     5881 gggtttcgtg aacttgcaaa gcattatgcc ttcctgacga aagcccagct aacagcaatg
     5941 attaacacta aagaactgac agagatctgc tatgactgca aaaactaaat ttaaaagtcc
     6001 tgcatttgag gctattcaca gcgcagcggc tggattgtcc agcgtcgatg ctatttctgc
     6061 cgaaacaatg cgtacttttg acaaagcctg cctgaccagc gtgcaagact tacaacctgt
     6121 tgagataaaa gcactgcggg aaaaattaaa agtgagtcag cccgtcttcg ctcactatct
     6181 caataccagc gtttcaactg tacaaaaatg ggaaagtggc gctaaacgcc ccaatggcat
     6241 ttcattaaaa ctgctcagca ttgtgcaaaa acacgggctg gatgtgctgc tgtaaataga
     6301 aagtagataa tgcgttcacc tactgtcagt tccgataccg gacgcggcgt gaacgcctta
     6361 tccactcgac aggtcaggcg cggtctgtag gctgataaga cgaagcggtt ttaatgcagc
     6421 cagtgaacgc cctcggcagg ttgaaagaat gcgttatagc gcatctgaaa agcattaata
     6481 tcctgcattt caggttcggt ttcgcgcaga aaacccagcg ccagcctgac ctgagcatcg
     6541 gtaatcggcg cggcaaggcg ggctttttgt aaaatccgat gccattgcag caggttctgt
     6601 tcgctaccat cgacaatgac aatttgccag atgagcaaca cctgcgcggc gttttgcagg
     6661 tgcgactcat ccggacgatg caaataacgt aaaaattcgt caccggctga ctttcccatt
     6721 tgatcgacca tcgactccac gggtacgaca tcgatattca gccgatcgct gagacggcga
     6781 atcgcttttc tggccccgga tgtcagcacc cgaaaaccaa ccacaaacac taccgccagc
     6841 gtggcgagca ttatccagat catgagttct cctgcgtaat cagaactcat gatatctgga
     6901 aaccctcgcc agttactaat ccaccagaat aatcttcagt gcaaacagag ctgcgaccac
     6961 caccacacac aggttcagat cgcgccagcg cccggtgcac actttcatga tgcagtacga
     7021 cataaagccg agtgcaatcc cttcggtgat cgagaaagta aagggcatca tcaccgtggt
     7081 aataaacgca ggcaccgatt cggtaaaatc atcccagtta acgcgcgcca ggctcgaagt
     7141 catcagcacg ccaacaaaga ttaacgctcc ggcggttgcg taaggaggaa ccatcgccac
     7201 cagcggtgag aagaacataa ccaacaggaa cataatgcca accacaaccg ccgttagccc
     7261 cgtgcgacca ccgactgcca caccagaagt actttcaata taggcagtaa cagacgaggt
     7321 accgataaac gcgcccgcca ccgaactgac gctatcaaca tacagcgcct tattcatatt
     7381 ggggaattta ccattactat ctattaagcc agctttatca gttacaccaa ttaatgttcc
     7441 tgatgaatca aataggttga tcagcataaa ggagaaaatg ataccggcga gttcaagtgt
     7501 taacgcgccg ctcaaatcta cttcaccaat gacgccgcta atatcaggcg gaatggaata
     7561 gacgccgcta aaatgaacat caccgaaaaa taatccgcag caggaagtga cgacgatgga
     7621 aaccagcacc gcggcatgaa aatgacgtga tgacaatacg gttataataa aaaaacctaa
     7681 tatacctaac aacacgccgt gagagcttaa atcgccaatc atcaccaggg tgtctttatt
     7741 ggcgacaata acgccagtgt tttttaatcc cattaaggca ataaataatc cgattccact
     7801 ggtgatacca atacgtaaac ttaatggaat gttggagatc atccagtacc ggatacgaaa
     7861 cagcgtgagc aaaaatagcc caattgcgcc ccagaatatc gcgcccatcc cggtctgcca
     7921 ggagataccc atcgccccca cgaccacgaa ggcaaagaag gcgttcagcc ccattgccgg
     7981 agccagcgcc acaggtaagt tagcaaatat ccccatcgca atgctgccga taccggcaat
     8041 caaacaggtg gtaacaaaca ccactttcgg gtccatttgt gccgcgccga gaatttgcgg
     8101 gttcacaaaa acgatgtaca ccatggttaa aaaagtggtc atccccgcaa tcaattctgt
     8161 gcggacggtg gtcccatgct gaggtagttt aaataatcgc gaaagcgtcc ctgattcatt
     8221 actcacgtaa tcggtattgt cattattcat ttttttgtcc attggaggag atttaatccc
     8281 ttgcttttgt aaaacaaatg acaacaacgg ttcagtgata attatcacat
//
LOCUS       DAPGOF010000197         8204 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.198, whole genome shotgun sequence.
ACCESSION   DAPGOF010000197 DAPGOF010000000
VERSION     DAPGOF010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.198"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            85..207
                     /locus_tag="RHV13_004099"
                     /pseudo
     CDS             85..207
                     /locus_tag="RHV13_004099"
                     /inference="COORDINATES: protein motif:HMM:NF024418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            274..5556
                     /locus_tag="RHV13_004100"
                     /pseudo
     CDS             274..5556
                     /locus_tag="RHV13_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001475310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
     gene            5767..6033
                     /gene="hipB"
                     /locus_tag="RHV13_004101"
     CDS             5767..6033
                     /gene="hipB"
                     /locus_tag="RHV13_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HDV1243571.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTSLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            6033..7355
                     /gene="hipA"
                     /locus_tag="RHV13_004102"
     CDS             6033..7355
                     /gene="hipA"
                     /locus_tag="RHV13_004102"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HDV1243572.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LIPEDETVTRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGNVRALAVERFDRRWNTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGAGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK"
     gene            7626..7894
                     /locus_tag="RHV13_004103"
                     /pseudo
     CDS             7626..7894
                     /locus_tag="RHV13_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT         2239 a         1751 c         2220 g         1994 t
ORIGIN      
        1 aaattagcca aattgacacc ttcccacctc caactaacac tcaatacaga cctctgacaa
       61 tttaataagt ttcattttta atgtctggcg actcatctct ttcgtggctg tattgtttgt
      121 atatggagcg tttatatgaa tagaatctat cgcgtgatat ggaattgcac cctacaggta
      181 tttcaggcct gctctgaatt aactcgcagg gtaggtaaaa catcaacggt taatttgcgt
      241 aaatcctctg gactgacaac gaaattcagt agattgacgc tgggtgtttt gctggcacta
      301 agcggttcag cgtctggtgc gagtctggaa gttgataatg gtcagattac caatattgat
      361 actgatgttg cttatgatgc ctacctggtt ggctggtatg gcactggagt tcttaatatt
      421 ttggctgacg gtaatgcctc cttaaccact attactacca gcgtcattgg cgctaatgag
      481 gattcggagg gtaccgttaa tgttttgggt ggcacctggc gattgtatga tagcggaaat
      541 aatgcaaggc ctttaaatgt gggtcaatcc ggaacgggga cgctgaatat taaacagaag
      601 ggtcacgtcg atggaggcta tttaagatta ggttcttcga caggaggcgt cgggacggtc
      661 aatgttgagg gagaggactc tgttctgacg accgaattat tcgaaatagg gagttacggt
      721 acgggctcat taaatattac ggataagggt tacgtcacga gttcaatcgt cgccatttta
      781 ggctatcagg cgggcagtaa tggccaggtt gtcgttgaaa agggtggcga gtggctaata
      841 aaaaataacg attcctcaat tgaatttcaa attggtaatc aaggaactgg ggaggcgact
      901 attcgcgagg gggggctggt tacggctgaa aatacgatta tcggtggcaa tgccaccggt
      961 gtcggaaccc tgaatgtgca ggatcaagac tctgtcatca cggtacgcag actctataat
     1021 ggatatttcg gtaatggcgc agtcaatatt tccaataatg gactaattaa taacaaagaa
     1081 tattcattgg tgggcgttca ggacggttcc cacggtgtcg tcaacgtgac cgataaaggg
     1141 cattggaatt tcctcggaac aggcgaagct ttccgctata tctatatcgg tgatgctggc
     1201 gacggtgaac ttaatgtctc gagtgaaggc aaagtagatt cgggaattat cactgcggga
     1261 atgaaagaaa caggcacagg caacattact gttaaggata agaactccgt tatcactaat
     1321 ctcggaacta atcttggtta tgacggccac ggcgaaatga atatcagtaa tgaggggctt
     1381 gttgtcagca acggaggaag ctcactcggt tatggagaaa ccggcgtcgg gaaggttagc
     1441 atcaccacgg gggggatgtg ggaggtcaat aagaatgtct ataccaccat tggtgttgcg
     1501 ggcgtcggaa acctcaatat tagcgatggc ggtaagttcg tatcgcaaaa tattactttt
     1561 ttgggcgata aagcaagcgg tatcggcaca ctgaacctga tggatgcgac atcgtcgttc
     1621 gatactgtgg gtatcaatgt cggtaatttt ggtagcggta tcgtaaatgt cagtaatggt
     1681 gccaccctta attcaacggg ctatggattt atcggaggaa atgcctccgg taaggggata
     1741 gttaatattt caacggatag tctctggaat ttaaagacgt cttctactaa cgcccaattg
     1801 ctacaagtcg gtgtattagg cacgggtgaa ctgaatatta ccaccggagg tatagttaaa
     1861 gcgcgtgata cacagatagc tctcaatgac aaaagtaagg gcgacgtgag ggtggatggg
     1921 cagaactctc ttcttgaaac attcaatatg tacgtaggga catctggtac gggtacgtta
     1981 accctgacga atagcggcac gctgaatgtc gaaggtggag aagtttactt aggtgttttt
     2041 gaacctgctg taggaacgct aaacattggt gcagctcacg gtgaggcggc ggcagatgcc
     2101 ggatttatca ccaacgcgac gaaagtagag tttggctctg gcgaaggtgt ttttgtcttt
     2161 aatcatacta ataacagtga tgccggctac caggtcgata tgctgattac aggtgacgat
     2221 aaagacggaa aagtgatcca tgatgcaggc catacggtgt tcaatgcagg gaatacttat
     2281 agcggtaaaa cgctggtcaa tgacggcctc ctgaccattg cgtctcatac ggcagatggg
     2341 gtaacgggca tgggttcgag tgaagtaacc attgcaaacc ccggtacgct cgacattctc
     2401 gcatcaacga acagtgcagg agattacacg ctgaccaatg cgctcaaagg cgatggcttg
     2461 atgcgagtgc agctgtcatc ctccgacaag atgtttggct ttacccatac aacagggact
     2521 gaattcgccg gtgttgccca actgaaagac agtaccttca ctctggaacg cgacaacacc
     2581 gctgcgctta ctcacgcgat gttgtagtct gacattgaaa ataccacatc ggtaaacgtg
     2641 ggagagcaat ccattggtgg actggccatg aatggcggta cgctcatttt cgatacggat
     2701 attcctgctg cgacgcttgc agagggatat atcagcgtcg atacgctggt tgtcggcgcg
     2761 ggtgactaca cctggaaagg ccgtaactat caggtaaacg ggacgggcga cgtgcttatc
     2821 gacgtgccta aaccgtggaa tgatcccatg gcgaataacc ctctgacgac gctcaatttg
     2881 ctggaacacg acgatagcca tgtcggcgtt caactggtga aggcgcaaac ggttattggg
     2941 tcgggtggct cattaacgtt acgtgattta cagggcgacg aggtggaagc ggacaaaacg
     3001 ttacacattg cgcaaaacgg aacggtggtc gccgagggtg attatggatt ccgcctcacg
     3061 accgcaccag gtgatggttt gtacgttaac tatgggctga aagcgctgaa catccatggt
     3121 gggcaaaagc tgacattagc cgaacatggc ggagcctatg gcgcaacggc cgatatgtcg
     3181 gcaaaaatcg gtggtgaggg ggatctggca atcaatacgg tgcgacaggt ttcgctttcc
     3241 aacggtcaga acgactatca gggggcaacc tacgttcaga tgggtacatt acgtaccgat
     3301 gcggatggtg cacttggcaa cacccgggaa ctgaacatca gcaacgcggc catcgtcgat
     3361 cttaatggat cgacgcagac ggtagagaca ttcaccgggc agatgggttc gactgttttg
     3421 ttcaaagagg gggcgctgac ggtaaataaa ggtgggatca gtcagggtga actgacaggt
     3481 ggcggaaacc tgaatgttac agggggaacg ctggctatcg aggggcttaa tgcacgctac
     3541 aatgcgttaa ccagcattag cccaaatgcg gaagtcagcc tcgataatac gcaggggtta
     3601 ggcagaggaa atattgccaa tgacggtctg ttaacgctaa aaaacgtgac tggcgaactg
     3661 cgtaatagcg taagcgggaa gggtatcgtg agcgcaaccg ccaggacaga tgtagagctg
     3721 gatggcgata atagccgctt tgtggggcaa ttcaacattg atacaggcag cgcgctcagc
     3781 gtcaacgagc agaaaaacct gggtgatgct tccgttatca ataatggcct gctcaccatc
     3841 tccactgagc gtagctaggc gatgacgcac agtatcagcg gtagcggtga tttgacaaaa
     3901 ctgggtaccg ggatcctgac tcttaacaac gattccgcgg cgtatcaggg tacgacggat
     3961 atcgtgagtg gtgaaattgc tttcggttcc gactctgcca ttaatatggc aagtcaacac
     4021 attaatatcc ataacagcgg tgtgatgtcg ggaaatgtca ccactgcagg tgatgtgaac
     4081 gttatgtctg gggggacact gcgtgtcgct aaaaccacaa tcggcggcaa cctggagaat
     4141 ggcggcacgg ttcaaatgaa tagtgaagga ggaaaaccgg ggaatgtact gaccgttaac
     4201 ggcaactata ccggaaacaa tggcctgatg acgttcaacg cgacgctggg cggcgataat
     4261 tcacccaccg ataagatgaa cgtgaaaggc gatacccaag ggaacactcg cgttcgggtt
     4321 gataacattg gcggcgtcgg tgcgcaaacg gtcaacggta ttgaactcat tgaggttggc
     4381 ggtaattctg caggtaattt cgcgctgacc accggaactg tcgaagctgg ggcttacgtc
     4441 tacacgttgg ctaaagggaa ggggaatgac gagaaaaact ggtatctgac cagtaaatgg
     4501 gacggcgtaa cgccagcgga tacacccgat cccatcaata atccccctgt tgtggatccg
     4561 gaaggcccat cagtttatcg cccggaggcc ggaagctata tcagcaacat tgccgcagcc
     4621 aactcgctgt ttagccatcg cttacacgac cgtctgggtg agccgcagta tacagattca
     4681 cggctttctc agggttcggc aagcagtatg tggatgcgtc atgtcggggg gcacgaacgt
     4741 ttcaggaccg gtgacggcca gctaaatact caggctaacc gctatgtatt gcagctaggc
     4801 ggagatttgg cgcagtggag tagcaacgcg caggatcgct ggcatcttgg cgtgatggca
     4861 ggctacgcca atcagcacag taatactcag agtaatcgtg tgggttataa atcggatggg
     4921 cgcatcagcg gttacagcgc tgggctgtac gcgacctggt atcagaacga tgcgaataag
     4981 accggcgctt atgttgacag ctgggcgctg tataactggt ttgataacag cgtcagttcc
     5041 gataaccgtt ctgttgacga ctatgattct cgcggtgtga cggcctctgt tgagggtggg
     5101 tatacctttg aagcgggaac atttagcggc agcgaaggga cgctgaatac ttggtacatc
     5161 cagccacagg tacaaatcac ctggatggat gtgaaagatt ctgaccatac ccggaaagac
     5221 ggaacacgca ttgaaacgga aggcgacgga aacgtgcaaa cgcgacttgg ggtgaaaacc
     5281 tacctgaaca gccatcacca gcgtgacgat ggtaaacagc gtgagttcca gccttacatt
     5341 gaagcgaact ggatcaacaa tagcaaagtc tacgccgtga agatgaatgg tcaaaccgta
     5401 agccgtgatg gtgcgcgtaa tctcggtgaa gtacgtaccg gggttgaggc gaaagtaaat
     5461 aacaacctta gcctgtgggg gaatgtcggt gtgcaactag gtgataaagg ctatagcgat
     5521 actcagggca tgctgggagt gaaatatagc tggtaaacag tataagccgc atgtcgagat
     5581 ggcatgcggc ttaatattgc caacttcaaa aggcttttaa agcctccctt ttatccgcga
     5641 tcgcggatat ctcagcgttt atcccgtaca gcggataaga tgtgtttcca gattgacttt
     5701 atcctcacta aaggataaaa ctgataatat ccccttaagc ggataaattt gctgtggacg
     5761 tatgacatga tgagctttca gaagatctat agcccaacgc aattagcgaa tgcaatgaaa
     5821 ctggttcgcc agcaaaatgg ctggacgcag agcgagctgg cgaaaaaaat cggcattaag
     5881 caggcgacga tttccaattt cgaaaacaac cctgacaata cctcgctcac gacatttttt
     5941 aagattttac agtcgcttga actctcaatg acgctatgcg acacgaaaaa tgcctcgcca
     6001 gaatcaacag aacagcaaga tctggagtgg taatgcccaa actagtcact tggatgaaca
     6061 accagcgggt aggcgagtta acgaagttag ccaacggcgc gcacaccttt aagtatgcac
     6121 cggagtggtt agccagccgt tatgccagac cgttgtcact ttcgctgcca ttgcagaggg
     6181 ggaatatcac ctctgatgcc gtatttaact tcttcgataa cttgttaccc gatagcccga
     6241 ttgtacgtga ccggatcgtt aaacgttatc atgccaaatc cagacaaccg tttgatttat
     6301 tgtcagaaat agggcgagac agcgttggtg ccgtgacgtt aatacccgaa gacgaaaccg
     6361 taacgcgtcc gataatggca tgggaaaagc ttactgaagc cagacttgaa gaagtattaa
     6421 cggcttataa agcagatatc ccgctaggca tgattagaga agaaaatgac tttcgcatct
     6481 cggttgctgg cgcgcaggag aagacagcgc tgctcagaat aggcaatgac tggtgcattc
     6541 cgaaaggaat aacgccgacg acgcacatca ttaaattacc gattggcgaa atcaggcagc
     6601 ccaatgcgac gctcgatctc agccaaagcg ttgataatga gtattactgt ctgctgctgg
     6661 cgaaagaact tgggttgaat gttccggacg cagaaatcat taaagcggga aatgtgcgcg
     6721 cgttagcggt cgaacgtttt gacaggcgtt ggaatactga gcgaacggtt ttacttcgct
     6781 tgccacagga ggatatgtgt cagacattcg gtttaccttc atcggtgaaa tatgaatcag
     6841 atggaggccc aggcatcgcg cgaatcatgg cttttttgat ggggtccagc gaggcgctga
     6901 aagatcgcta tgattttatg aaattccagg tctttcagtg gttgattggc gcaacggatg
     6961 gccatgcaaa aaacttctcc gtatttattc aggctggcgg cagttatcga ctcacgccat
     7021 tttacgacat catttcagca tttccggtat tgggtggcgc gggaatacac atcagcgatc
     7081 tcaaactggc aatggggctt aacgcatcca aaggcaaaaa aacggcaatc gataaaattt
     7141 atccgcgaca ttttttggcg acagcaaagg tgctgagatt cccggaagtg cagatgcatg
     7201 aaatcctgag tgactttgcc agaatgattc cggcagcact ggataacgtg aagacttcat
     7261 taccgacaga ttttccagag aacgtggtga cggcagttga aaccaatgtg ttgaggttgc
     7321 acggtcggtt aagccgagaa tacggtatta agtaagagta agatatgggg tttggtcgtt
     7381 gttaatgagc aggacaatca tgaccgccaa cgactagatt ctcccttggt ttatgttagt
     7441 gcgcataatg tatattatgt taaatcgtat ctgagtgata cgaagtgatt ttccggtagt
     7501 cgttattcgt tcagggttcc agaacttaag cctctcctct ttcataattg gcctgtgcat
     7561 gccggatata ggctggctgg tatgcagatt tgccgtttgc cgcatcttcc cagcgttgaa
     7621 gactcaagct ggtaaaccgc gtgcaaatga gcacgccctc tttccatcaa cacttcaggc
     7681 agcttgcggg aatgagtcca ctccggtatc agaaatggct acgtctcaat gaagtcaggc
     7741 gaccgatgct gaatgaacat tacgatgtca ccacagcagc ctatgctgtc ggttacgaaa
     7801 gctatcccat ttcagtcggg aatattcgcg gatgtttgga gagtcatcca agagagatat
     7861 taccaggtta agaaagtctg tcggtcagct ttaataaacc ctgcacttat ctgtttagag
     7921 caattcggtg ttagtttcag caagcaaaca ttaaccatag ctaatgattt atagccatat
     7981 taaccattgg tagcccttga cctttcctga cacttccctt gttcgcaata atattctgtt
     8041 tagtcacggt ttataaataa catgcataat gaaatcgtca tgttatttag cgacataaaa
     8101 tgtttttgca aatactctct tcaacaagtg cagcaataaa cttgttgtga ttaggtgtcc
     8161 ttgtgattta aagatattat cgttcttcgt gaaatgtaat aacc
//
LOCUS       DAPGOF010000198         8141 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.199, whole genome shotgun sequence.
ACCESSION   DAPGOF010000198 DAPGOF010000000
VERSION     DAPGOF010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.199"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            22..519
                     /gene="ftnA"
                     /locus_tag="RHV13_004104"
     CDS             22..519
                     /gene="ftnA"
                     /locus_tag="RHV13_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HDV1243573.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDAQN"
     gene            complement(557..796)
                     /locus_tag="RHV13_004105"
     CDS             complement(557..796)
                     /locus_tag="RHV13_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HDV1243574.1"
                     /translation="MDSIHGHEVLNMMIESGAQYTHASLEAAIKAHFGDQTRFHTCSA
                     EGMTAGELVAFLAAKGKFIPCEEGFSTDKSKICRH"
     gene            988..2199
                     /gene="tyrP"
                     /locus_tag="RHV13_004106"
     CDS             988..2199
                     /gene="tyrP"
                     /locus_tag="RHV13_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HDV1243575.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATTGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREIVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2261..2926)
                     /gene="yecA"
                     /locus_tag="RHV13_004107"
     CDS             complement(2261..2926)
                     /gene="yecA"
                     /locus_tag="RHV13_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HDV1243576.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3122..3208)
                     /locus_tag="RHV13_004108"
     tRNA            complement(3122..3208)
                     /locus_tag="RHV13_004108"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3172..3174),aa:Leu,seq:taa)
     gene            complement(3221..3294)
                     /locus_tag="RHV13_004109"
     tRNA            complement(3221..3294)
                     /locus_tag="RHV13_004109"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3260..3262),aa:Cys,seq:gca)
     gene            complement(3348..3423)
                     /locus_tag="RHV13_004110"
     tRNA            complement(3348..3423)
                     /locus_tag="RHV13_004110"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3388..3390),aa:Gly,seq:gcc)
     gene            complement(3575..4123)
                     /gene="pgsA"
                     /locus_tag="RHV13_004111"
     CDS             complement(3575..4123)
                     /gene="pgsA"
                     /locus_tag="RHV13_004111"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDV1243577.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(4180..6012)
                     /gene="uvrC"
                     /locus_tag="RHV13_004112"
     CDS             complement(4180..6012)
                     /gene="uvrC"
                     /locus_tag="RHV13_004112"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDV1243578.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGANRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(6009..6665)
                     /gene="uvrY"
                     /locus_tag="RHV13_004113"
     CDS             complement(6009..6665)
                     /gene="uvrY"
                     /locus_tag="RHV13_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HDV1243579.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            6961..7137
                     /locus_tag="RHV13_004114"
     CDS             6961..7137
                     /locus_tag="RHV13_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243580.1"
                     /translation="MIKIFIGHYINAFNSTVDDTLKKQPLLFLTKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            7124..7348
                     /locus_tag="RHV13_004115"
     CDS             7124..7348
                     /locus_tag="RHV13_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HDV1243581.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(7416..8138)
                     /gene="sdiA"
                     /locus_tag="RHV13_004116"
     CDS             complement(7416..8138)
                     /gene="sdiA"
                     /locus_tag="RHV13_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HDV1243582.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMETAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFNE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILRWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
BASE COUNT         2015 a         2078 c         1912 g         2136 t
ORIGIN      
        1 taacctttgt ggagcactat catgctgaaa ccagaaatga ttgaaaaact taatgagcag
       61 atgaacctgg aactgtactc ttcactgctt tatcagcaaa tgagcgcctg gtgcagctat
      121 cataccttcg aaggtgctgc cgcgttcctg cgccgtcatg cccaggaaga gatgacgcat
      181 atgcagcgtc tgtttgatta cctgactgat accggcaatt taccgcgtat taataccgtt
      241 gaatctccgt ttgctgaata ttcctcactt gatgaattat tccaggaaac ctataaacac
      301 gaacaattaa tcactcagaa aattaacgaa ctggctcatg ctgcaatgac caatcaggac
      361 tacccaacat ttaatttcct gcagtggtat gtttctgagc agcatgaaga agagaaactg
      421 ttcaaatcga ttattgataa attaagtctg gcaggcaaaa gcggcgaagg tctgtatttt
      481 atcgacaaag aactctctac cctcgacgca caaaactaat cttctctggc ggcagatatt
      541 ctgccgcctt ttcatatcag tgacggcaaa tcttactttt atcggttgag aacccttctt
      601 cgcagggaat aaatttgcct tttgccgcaa gaaaagccac caactctccc gcggtcatcc
      661 cttcagccga gcaagtatga aaacgtgtct gatcaccaaa atgcgcttta atcgcagctt
      721 ccagactggc atgcgtatat tgcgcgcctg attcaatcat catatttaac acttcatgac
      781 cgtgaataga gtccatcgtt cctcttcaaa aaaaagccta gcgtagcgat tgccgcttat
      841 gaagactttg cgccagcgca ggactgaatg ctttttattg tacatttata tttacaccat
      901 atgtaacgtc ggtttgacga agcagccgtt atgccttaac ctgcgccgca gatatcactc
      961 ataaagatcg tcaggacaga agaaagcgtg aaaaacagaa ccctgggaag tgtttttatc
     1021 gtggcgggaa ccacaattgg cgcaggcatg ctggcaatgc cgctggctgc ggccggtgtt
     1081 ggttttagcg ttacgttaat cttgttgatt gggctttggg cgttgatgtg ctacacggcg
     1141 ctattactgc tggaggtgta ccagcatgtt ccggcagata ccggtctggg cacgctggca
     1201 aaacgctatc tgggacgcta cggtcagtgg ctgacgggct tcagtatgat gttcttaatg
     1261 tatgctctga ctgcggcata catcagcggt gccggtgaat tgttggcctc cagcatcagc
     1321 gactggacag gtatttcaat gtcggcaacc actggcgtgc tgttgttcac ttttgttgcc
     1381 ggtggcgtgg tttgtgttgg aacatcgctg gtcgatttat ttaaccgttt tctgttcagc
     1441 gccaaaatta tttttctggt ggtgatgctg gtattgctgc taccgcatat tcacaaagtg
     1501 aatcttttaa ccctgccgtt gcaacagggg ctggcactgt ctgcaatccc ggtgattttt
     1561 acgtcgtttg gttttcacgg tagcgtgccg agtattgtca gctatatgga tggcaacatt
     1621 cgtaagctac gctgggtgtt tataaccggt agtgcgatcc ccctggtggc atatattttc
     1681 tggcaggtgg cgacgcttgg cagcattgat tcaacaacct ttatgggatt gctggctaat
     1741 catgccggat taaacgggct gttacaggcg ttacgcgaaa tagtggcctc tccgcatgtt
     1801 gagctggcag tgcatttatt tgctgattta gccctcgcca cgtcatttct cggcgttgcg
     1861 ttaggcttat ttgattatct ggctgatttg tttcagcgtt caaataccgt tggtggaaga
     1921 ttgcaaactg gtgcaattac ctttctgccg ccgttggcgt ttgcactgtt ttatccacga
     1981 ggatttgtga tggcgctggg ttacgccggt gtggcgctgg cggtactggc attgattatc
     2041 ccttcgctgt tgacctggca aagcagaaag cacaatcctc aagcgggtta ccgggtcaaa
     2101 ggtggtcgtc cggcgctggt ggtggtgttt ctctgtggta ttgctgtgat tggcgtgcaa
     2161 tttttgattg cggcagggtt gttaccagaa gtggggtgat cagatagcct caaattcctt
     2221 attgggtgcc agaattaacg ctgacaccca atttgacttc ttaatgcagg cagcactgct
     2281 taaatttctt accactaccg cacgggcaag gatcgttacg ccccggtttc tcttctgctt
     2341 tgatcggttg ctgaacagct ttttcctgcg gatgcgccat ccagtacgca tgtagatcaa
     2401 gcgccgccag tcgaatggca tctacgctct cttcaaacgc ttctggcgac atcttctcta
     2461 cccgctcgaa gttttcctca gtaccgtgca gcgcaatcgc ctccagcgct ggttttaacg
     2521 aatcgggcaa tgttgaccag tcagaaagtg ccacgccccg catatagcca aagcaccact
     2581 cctcaacaat cgtcagctcg ctgccatcaa cttctcgcaa gccgaataac ggctcaaact
     2641 gctccgggaa ttcgttcaga cgctctgcgg tatcggccat atgttgaaaa gccaggttca
     2701 taaagcgcgt catctctttc tctgacgccc agcgcggcac atagtcagcc ccaccccaca
     2761 cggcaaccag ccactgttcc ggttcaatct cttgcggaga actcaacacc gccgtcaata
     2821 aaccgtccag ctccgccaca tcaaggatgg cgtggtcagt gttgtatttg gtcagaatat
     2881 cgtccagcca ttccaactca ctttcgttta acggtcccgt tttcatacgc ttttccttgt
     2941 ggatctcaac tcgccagcac ctatcttaca tgccggtccg tatcagagat actttttgag
     3001 tggctttgct ggtgattaaa aattaaggag ggtgtaacga caagttgcag gcacaaaaaa
     3061 accacccgaa ggtggtttca cgacactgct tattgctttg attttattct tatctttccc
     3121 atggtacccg gagcgggact tgaacccgca cagcgcgaac gccgagggat tttaaatccc
     3181 ttgtgtctac cgattccacc atccgggctc gggaagaaag tggaggcgcg ttccggagtc
     3241 gaaccggact agacggattt gcaatccgct acataaccgc tttgttaacg cgccaaattc
     3301 ttcaggcctt tcagccagac atccgctgac gccgatgtct tttaaactgg agcgggaaac
     3361 gagactcgaa ctcgcgaccc cgaccttggc aaggtcgtgc tctaccaact gagctattcc
     3421 cgcattcatc aagcaatcag ttaatcactt gattttatta tcgtctggca aacagtgccg
     3481 ccgttcgatg cgttgcattc tacttacctg gcgcgatgag tcaacgatat ttttcaccac
     3541 ttttgatcgt ttgctgaaaa ttgcgccgaa acgatcactg atcaagcaaa tctgcacgcg
     3601 cagcgctcaa atattgcaac attgaccaca gagtcagtac cgcagccaca aagaaaagtg
     3661 caataccggc gtactcaacc caaatgttcg gacgccacag cagccatgcc aacgccacca
     3721 tctgggcagt ggttttcact ttcccaatcc aggagacagc cacgctactg cgtttaccca
     3781 actccgccat ccattcgcgt agcgcagaaa taataatttc acgggcgatc atcgttgccg
     3841 ccggtaaggt cacccaccag ctgtggtaat gctcggttac cagcaccatg gcgatagcca
     3901 cgagaacttt atctgctaca gggtcaagga aagcaccaaa ccgggtactc tggttccagc
     3961 ggcgtgccag aaaaccatcg aaccagtcag tcaccgccgc gacgcagaaa atgagcgcgg
     4021 cggcaaacgg cgaccaggtg acaggcagat aaaagaccaa tacaaagaat gggataagga
     4081 tgacacggaa cagtgtaagc aacgtaggga tattaaattg cataatgacg ggtaactatc
     4141 tgttgtcagt aagattaccc ctatgttgct acagagacat caatgtttca acgaccagaa
     4201 gatcttttct gccagacctt gcgaaatacc cggcactttt gcaatttcct cgacgctggc
     4261 gttacgtaaa ccttgcaaac cgcccatata tttcaacaac atttgccggc gttttggccc
     4321 gacgccttca atggtttcca gggaactggt atttttgacc ttcgcccgtt ttttacggtg
     4381 cccgccaatc gcgtgatcat gtgattcatc gcgaatatgc tggataacat gcagcgcggg
     4441 agaatctggc ggcaaactga atccctcacc ttccggctca aagaacagcg tttccagccc
     4501 agccttacga tttgctcctt tggcaacgcc aagtagcagc ggatgatttt tatcccatga
     4561 gacatccagt tcggcgaaga catttttcgc ctgcgcaagc tggcctttgc cgccgtcgat
     4621 aagtatcaca tccgggatct tactgtcgtc gatggcttta ccataacgcc gacgcagcac
     4681 ctgattcatc gccgcataat catcgcccgg cgtgatgcct gtaatgttat agcgccgata
     4741 ctccgcacgc agcgggccgt tagcatcaaa caccacacag gaagcgacgg tttgttcgcc
     4801 catggtatgg ctgatgtcaa agcactccat ccgcttcact tccggcaatt tcaacacgct
     4861 ggcaagcgcg gtcagtcgct ggtgaacggt agattgctgc gaaagtttgc tggttaaggc
     4921 cgtcgccgca ttggtgcgcg cgagtttcag ataacgcgcc ctatcgccgc gaggtttggt
     4981 ttgaacatta atcttgcgtc ccgccagttc tgaaagggaa tcggcgagca gcgttttatc
     5041 gctaagatta aaatcgagca ggatctcacc cggtaaggtg cgcatctggc tgccttgtaa
     5101 atagaactgg cctacgaagg tttctaccac ctcgctcagt tccgtaccgc caggcacttt
     5161 cgggaaatag ctgcggctgc cgagcacttt gccctgacga atgaacaata cgtggacaca
     5221 agccatgccc gcatcgaacg ccacaccaat aacgtcgagg tcgtcgccgg tattggaaac
     5281 gaattgtttt tcggtgacgc gtcgcaccgc ctgaatttgg tcgcgaatac gtgcagcttc
     5341 ttcaaactcc agattctggc tggcagtttc catacgacta atgagttgcg taagcacctg
     5401 atcatctttg ccagacaaaa acaggcgcac atactcgacc tgctgagcgt attcttcttc
     5461 actcaccagt ccttcaacgc acggtcccag acagcgccct atctgatatt gcagacacgg
     5521 acgcgagcga ttgcgataaa cactattttc gcactggcga atggggaaaa tcttttgcag
     5581 tagcgccagt gtttcacgta cggcatagcc attcgggaac gggccgaaat attcaccttt
     5641 ggcatgcttc gcaccacgat gcatcgccag acgcgggtgg gtatcgccac tcaggaagat
     5701 aaaaggatat gatttatcat cgcgtagcaa aacgttgtaa cgcggctgat agagtttgat
     5761 gtagttgtgt tccagcaaca gcgcttcggt ttctgtatga gtaaccgtta catcaatttg
     5821 ctggatctgg gcgaccagcg cttcggtttt gcgcgaagcg aggttgctac ggaaatagct
     5881 ggaaagccgt tttttcaggt ctttcgcttt gccgacatag ataaccgtac caccagcatc
     5941 gtacatgcga taaacgccag gctggctggt tacggttttt aaaaacgctt ttgcgtcaaa
     6001 ctgatcactc actgacttga taatgtctcc gcattacaca gaccatggcg aattgccagg
     6061 tgagtcagct caacatcgcc atgaatgttt agtttactga acatacgata gcggtagctg
     6121 ttcaccgttt tcggactgag attgagctgt tctgagatct cattgacctt ctggcccttg
     6181 gtgatcatca gcataatctg caattcacgt tcagacaaac tggcaaatgg gctttctgtt
     6241 ttttctggtt cgatctggct taacgccatt tgttgagcga tgtcagaagc aatgtaacgc
     6301 tgccctgaat agacagaacg gatcgcactc acgacttcct gcggagccgc gcctttgctg
     6361 aggtagcccg cagcaccggc ctgcatgact ttcgctggta aagggttttc tgtatggacg
     6421 gtaagcatga tgattttgac atcagctgtg gaacgcgcga ttttacgcgt cgcctcaaga
     6481 ccgccaatgc ccggcatact catgtccatt agcaccacgt caacggcatt tgcccggcac
     6541 cacttaacgg cgtcttcacc gcacgatgcc tcaccgacga cttttatacc ctttatatct
     6601 tccagaatgc gtcgtatccc tgcgcgcacc agttcgtggt catcaacaag tagaacgttg
     6661 atcaaaggaa tatctccaga aatagggata acgctactga tagttagtca ttcgtatatt
     6721 agcggttttt attgcaactt tgaaacgtta aaaatggtgg gtattcgatt tttctctcgt
     6781 ttttgtcaat tcgattgtcc acagttatgg tcacgcccgt caccaacggc ttaccggatc
     6841 gtttcacagt aagccctgcg acgaaatgtg acaaaaatta cctttattca gcaaaaatga
     6901 aaatcagccc gctgaatatg taacattaat taacccgaac aatacgggca aaaaacattc
     6961 atgataaaaa tatttattgg tcattatatt aacgcattta atagcaccgt tgacgacacg
     7021 ctcaaaaaac aaccactgct atttttaaca aagcttatgg tatactccgc cgccttaaca
     7081 tttttcaccg caaattttca ttgcaacatg acgaggaaaa taaatgagta cgcctgattt
     7141 ttctactgcc gagaataatc aagaactggc aaatgaagtc tcctgcctga aagcgatgct
     7201 gacgctgatg ctgcaggcga tgggacaagc tgacgcgggc cgcgtgatgt taaagatgga
     7261 aaaacagctt gcgctgatcg aagacgaaac ccaggctgca gtattttcca aaacggttaa
     7321 gcaaattaaa caagcctacc gtcagtaata taaaaccggc tgatagcgtg cctttcagcc
     7381 ggtttttgca tctggcacgc aggacagaaa agagatcaaa ttaagccagt agcggccgcg
     7441 taacaggcaa cctgggtctt atttggtgca ttaatttttt tctgcatgtt tttctggtgg
     7501 aaattgaccg tattctcaga gattgacaaa atcatcgcta tctctgctga tgtcttccct
     7561 tccgccgtcc acctcagaat ttctttttcg cgcttgctga aattcatctc tggcgtcatc
     7621 actatttcat catttaaacg catcagagcc atcagacttt cgcgcaccag taactgcatt
     7681 tttaattgca gttcatcact aagaatgggt atttcgcgcg cgctgcaacg ggaaaaggac
     7741 aaaaagccca gcgcccggtt tggcagcatt aaatactgag tgacaccgcg gcgtaaacca
     7801 tgtgcgcgcg cggcttccca taacggctgt gcttcgttga ataagtcatc attccacatt
     7861 agatggccct gactaaagtt ttcagggttc agtaccggat caattgcgag aaagtttttt
     7921 gcctgataat aactaaccca cgcctcaggg taattggtgt agaaagccac tttaggtcga
     7981 gtgaatggta ccgggtggcg gacacataac gaatagtaat cgtactccag ctgctgagcc
     8041 tgaagctcaa tttcatggta gacctcttct gcggtctcca tcctctgaaa acgcaacagc
     8101 atcgtgcgac gccagctgaa aaaatccgta tcctgcatag t
//
LOCUS       DAPGOF010000199         8096 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.200, whole genome shotgun sequence.
ACCESSION   DAPGOF010000199 DAPGOF010000000
VERSION     DAPGOF010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8096
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.200"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            41..1207
                     /locus_tag="RHV13_004117"
     CDS             41..1207
                     /locus_tag="RHV13_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDV1243583.1"
                     /translation="MIEHYIRGFEEELREIRHQIHENPELGLQEFKTSALVAEKLRQW
                     GYEVEQGLATTGVVATLKVGDGEKSIGLRADMDALPIYENSGKPWASKHPGLMHACGH
                     DGHTTILLGAARYFAETRRFNGTLRLIFQPAEEMINGGEIMVKEGLFDRFPCDVIFGM
                     HNMPGLPVGKFFFQPGALMASMDQFHITVRGCGGHGAIPHKAIDPVLVAAHITTALQS
                     IVSRNVDPLEAAVITVGSIVAGEAANVIPDSAEMKISVRSLSRDTRQLLLTRIPALAQ
                     AQAASFGATAEVTHVNGTPVLVNDEEMARFAWQVACKTFGEDRAEFGIKPLMGSEDFS
                     FMLEAQPKGGFLLFGNGDVGEGSCMVHNPGYDFNDASLVPASSYWGALVEAWLQ"
     gene            1250..2566
                     /locus_tag="RHV13_004118"
     CDS             1250..2566
                     /locus_tag="RHV13_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1243584.1"
                     /translation="MTAIVNETVSVKKKPSGRRVIFASAFGNALEFFDFGVYNFFVVY
                     ISTLFFPPSADKNVALLLAFATFGVSFFMRPLGGIIVGAWADRFGRKPAMVFTIALMS
                     LGTLMIGIAPTYETAGYWGTATLVLARLIQGVAAGGEVGASMSLLVESAPANRRGFYS
                     SWSLATQGLATTFGGVVALGLSAWLPFATGSETVMAEWGWRVPFFIGVLLAPIGCWLR
                     LSLENDVPEPVRNKKAATSESAFSLLLQHKATIVNGVLLAIGSTVATYISLFYYGTWA
                     AKYLAMPQHYSHAAMLLAGVITFVGALLVGMLCDSVGRKKLILISRVMVLICSWPSFW
                     LLVNYPSPGMLLTVVFVMVSFTTLGGVPVMVLISELLPKRIRALGFALVYSIGVAIFG
                     GFAQYFATQSIVLLDSLPAPAWYLGGGTLLSMLALLYVKEPAKELQ"
     gene            2670..3320
                     /gene="fabR"
                     /locus_tag="RHV13_004119"
     CDS             2670..3320
                     /gene="fabR"
                     /locus_tag="RHV13_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HDV1243585.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            3320..3679
                     /locus_tag="RHV13_004120"
     CDS             3320..3679
                     /locus_tag="RHV13_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HDV1243586.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(3719..4819)
                     /gene="trmA"
                     /locus_tag="RHV13_004121"
     CDS             complement(3719..4819)
                     /gene="trmA"
                     /locus_tag="RHV13_004121"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HDV1243587.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      4933..5123
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            5188..7071
                     /gene="btuB"
                     /locus_tag="RHV13_004122"
     CDS             5188..7071
                     /gene="btuB"
                     /locus_tag="RHV13_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HDV1243588.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFQQPVNTVLA
                     PTDIVTREDIQRWQSKDLNDVMSHLPGVNISQTGGMGTSSSLYVRGTESRHVLVLIDG
                     VPMARAGIDNAIDISQFPVSLVQRVEYIRGPRSAVYGSGAIGGVVNIITMSNDEKAQF
                     NAGMGTNGYQAYDGVINKRFGDTMVTAAGAYQTTKGFNIQPGSPYSGDSDRDGYRNKL
                     FWGGLQHKFNDNFSGFFRGYSYTANNDYDQGSMYGSATGNEEAQNYNQSWDTGLRYNS
                     GIYSSQLIANYQRLKNYNYTNNLGRYAGDATLDDMEQRYIQWGNSIEVGHGAISGGID
                     WKQEKLTSSSITKSDDYERDTTGLYLTGQQQISNVTLEASGREDHDEQFGWHGTWQTA
                     AGWEFIDGYQATLSYGTSFLSPSLGQQYGAARFASIYGPGIAANPNLKPEESKQWEIG
                     IEGVTGTLDWRLSGYRYEIQNLIDYKSINNVNQYINVKSATIKGLEWTGNITTGPIEH
                     RLTLQYVNPRDDETDKVLYRRAKQQVKYELTGQAYGFEWDVSYQYIGQRYDNAYDETS
                     GSSHTVKLGGVSLWDLAVAYPVTSNLTVRGKIANLFDKDYETVYGYQTAGREYTLSGS
                     YTF"
     gene            7016..7873
                     /gene="murI"
                     /locus_tag="RHV13_004123"
     CDS             7016..7873
                     /gene="murI"
                     /locus_tag="RHV13_004123"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDV1243589.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         1891 a         1879 c         2180 g         2146 t
ORIGIN      
        1 cgttaattag cctgtttata agtgaataaa ggaatagccg atgatcgaac actatattcg
       61 tgggtttgag gaagaactgc gtgagatccg ccatcagatc cacgaaaacc cggagctggg
      121 attacaggaa tttaaaacca gtgctttggt agcggaaaag ctgcgccagt ggggttatga
      181 ggttgagcaa gggctggcga caaccggcgt ggtagccacg ttaaaagtgg gcgacggaga
      241 gaaaagtatt ggcctgcggg cggatatgga tgcgttgccg atttatgaaa atagtggcaa
      301 gccctgggcc agtaaacatc ctggtttaat gcacgcctgc gggcatgacg ggcataccac
      361 catactgctg ggggccgcgc gttattttgc cgaaacccgc cgttttaacg gcacgttacg
      421 tcttatcttc cagcctgcgg aagagatgat taacggcggt gagatcatgg ttaaagaggg
      481 gctttttgat cgtttcccct gcgatgtcat tttcgggatg cacaacatgc cgggtttgcc
      541 ggtcggcaag tttttcttcc agcctggggc gctgatggcg tcaatggatc agttccatat
      601 tacggttcgt ggttgtggcg gacacggtgc gatcccgcac aaagccatcg atccagtgct
      661 ggttgccgca catatcacca ccgcattaca aagcattgtg tcgcgcaatg tcgatccgct
      721 ggaagcggcg gtaattaccg ttggcagcat tgttgcgggt gaggcggcta acgtcattcc
      781 tgacagtgct gaaatgaaaa tcagcgtccg ctctcttagc cgcgataccc gacaacttct
      841 tctgacccgt attcctgctc tggcacaagc ccaggctgcc agctttggtg ctacggctga
      901 ggttacgcat gttaacggta caccggtatt agtgaatgac gaggagatgg cgcgcttcgc
      961 ctggcaggtg gcgtgtaaaa cgtttggtga agatcgggcc gagtttggca tcaagcctct
     1021 gatgggcagt gaagatttct ctttcatgct ggaagcccaa cccaaaggcg gtttcctgtt
     1081 attcggtaat ggcgatgtcg gggagggttc ctgcatggtg cataacccgg gttacgactt
     1141 caacgatgca agtctggtcc cggcaagcag ctactggggt gcactggtag aagcctggct
     1201 gcaataaata aaacaacaca caacaacact ttttggcaag gaacatggaa tgacggcgat
     1261 cgtaaatgaa accgtgagtg tgaagaaaaa gcccagcggc aggcgggtga tttttgcctc
     1321 ggcgtttggc aatgcgctcg aattttttga ttttggcgtt tacaactttt ttgtggtcta
     1381 tatcagcacg ctattttttc cgcccagtgc agataaaaac gtcgcgctac tgctggcgtt
     1441 tgccactttc ggcgtgagct tttttatgcg cccgctcggc ggcattattg tgggggcctg
     1501 ggccgatcgt ttcggacgca aacccgcgat ggtgttcacc attgcgctga tgagtcttgg
     1561 tacactgatg atcggcatcg caccgacgta tgaaacggcg ggctattggg gaacggcaac
     1621 tctggtactt gctcgtttga ttcagggtgt tgcagcaggc ggtgaagttg gggcttcaat
     1681 gtcgctactg gtggagtcag ctccggcaaa tcgtcgcggt ttttacagca gttggtcact
     1741 ggcgactcaa ggcctggcaa caacttttgg aggtgttgtc gctttaggtt taagtgcatg
     1801 gctgcccttt gcgacaggtt cagaaaccgt catggctgaa tggggctggc gtgtgccatt
     1861 ttttattggc gtgttattag cacctatcgg ttgctggttg cgtctgagcc tggaaaatga
     1921 cgtacctgag cctgtgcgca ataaaaaagc tgctaccagc gaaagcgcgt tttctctgct
     1981 cctgcaacat aaggcgacta ttgttaatgg cgttttactc gctattggta gcacggtcgc
     2041 aacctatatc tccctctttt attacggaac ctgggcggcg aagtatctgg cgatgcctca
     2101 acattattct catgcagcga tgttactggc gggcgttatt acgtttgtgg gtgcgctgct
     2161 ggtggggatg ttgtgcgatt cggtcgggcg taaaaagctg attttaatct cccgtgtgat
     2221 ggtgctgatc tgtagctggc cgtcattctg gctgttggtg aattacccaa gcccaggcat
     2281 gttgctgacg gtggttttcg tgatggtcag ctttaccacg cttggcggtg tgccagtgat
     2341 ggtgttgatc tctgaactgt tgccgaaacg gattcgggca ttgggttttg cgctggttta
     2401 tagcattggt gtggcaatct ttggcggttt tgctcaatat ttcgctacgc aatccatcgt
     2461 gttgctggat agtttgccgg ctccggcatg gtatctgggt ggaggcacgt tgctgtcgat
     2521 gctggcgttg ttgtacgtaa aagaaccggc caaagaattg cagtaaatat gttttattgc
     2581 gttaccgttc attcacaata ctggagcaat ccagtatgtt cattctctgg tatagtgcca
     2641 gcagtacttt tggcaaggat tcagacatcg tgatgggcgt aagagcgcaa caaaaagaaa
     2701 aaacccgccg ttcgctggtg gaagccgcat ttagccaatt aagtgctgaa cgcagcttcg
     2761 ccagcctgag tttgcgtgaa gtggcgcgtg aagcgggcat tgcccccacc tctttttatc
     2821 ggcatttccg cgacgtagac gaactgggtc tgaccatggt tgatgagagt ggtttaatgc
     2881 tacgccaact catgcgccag gcgcgtcagc gtatcgccaa aggcgggagt gtgatccgca
     2941 cctcggtctc cacatttatg gagttcatcg gtaataatcc taacgccttc cggttattat
     3001 tgcgggaacg ctccggcacc tccgctgcgt ttcgtgccgc cgttgcgcgt gaaattcagc
     3061 acttcattgc ggaacttgcg gactatctgg aactcgaaaa ccatatgccg cgtgcgttta
     3121 ctgaagcgca agccgaagca atggtgacaa ttgtcttcag tgcgggtgcc gaggcgttgg
     3181 acgtcggcgt tgaacaacgt cggcaattag aagagcgact ggtactgcaa ctgcgaatga
     3241 tttcgaaagg ggcttattac tggtatcgcc gtgaacaaga gaaaaccgca attattccgg
     3301 gaaatgtgaa ggacgagtaa tgaaacaagc aaatcaagat agaggtacgc tgctgctggc
     3361 gttagttgct ggcttatcga ttaatggtac tttcgcagcg ctgtttagct ccattgtgcc
     3421 attttctgta ttcccgatta tttccctggt gctgacggtt tactgcctgc atcaacgtta
     3481 tcttaatcgc actatgccgg taggcttgcc gggtctggca gctgcctgtt ttattctcgg
     3541 cgtactgctg tacagcacgg tagttcgtgc ggaatatccg gatatcggct ctaacttctt
     3601 cccggcagta ctctccgtca ttatggtgtt ctggattggc gcgaagatgc gtaaccgtaa
     3661 gcaggaagtt gctgagtaat cggtattatg ccggatgctg gcgcatccgg catgggtttt
     3721 acttcgcggt cagtaatacg ccgcactcca tatggtgcgt gtaggggaac tgatcaaaca
     3781 gagccagacg ttcgaccttg tgcgtctggc ttaatgtttc cagattcttg cataacgttt
     3841 ccgggttaca ggagatgtac aaaatacgcg gatacgcctg caccattttc tcggtttcac
     3901 tgtccagacc gctgcgcggt gggtcgacaa aaatggtttc gcactgataa ctctttaagt
     3961 cgatcccttg caggcggtta aactcgcgca caccattcat cgcctgagta aattcttctg
     4021 ccgccatacg aataatttgt acgttatcaa tatggttagc tgcgatgttg tattgcgcag
     4081 cagcaaccga cggcttagcg atttcggtgg ctaatacccg atcaaaattg cgcgccagcg
     4141 ctaatgaaaa gttaccgttg ccgcagtaca gctccagtaa atcgcctttt gagcctttgg
     4201 ttacgtccag cgcccattcc agcatctgaa tattcatcgc cgcgttcggc tgggtaaagc
     4261 tgttttctac ctgacggtag atcatctctt tccctgcgac cggcagacgt tcatcgatgt
     4321 aatcctgatc cagcgcgatt ttggttttcg ttgcccgacc aatcagatgc acattcagat
     4381 tctgtgctcg cagtgcatcg cgcagggctt ccgcttcctg acgccactca tcatccagct
     4441 ttttatggta cagcagggaa accaccgcct gattactcag cgtagtgagg taatcaatct
     4501 ggaacaactt gtggcgcaga acgggattat tacgcacacc cgcaatcatc gccgtcatca
     4561 actggttgat aagttcactg gcggcgggga agctatccac gcggatgcgg cttttggttt
     4621 gttgatcgaa aatgatgtga tacaggtcat cgccatcgtg ccagatgcgg aactccgcgc
     4681 gcatccggta atgactgacc ggcgagcgaa acacttccgg aaccaggtca gaaaacggtg
     4741 ccatcatact ttgcaaacgt accacttttt cggctaactg cgcttcatac tgttctgttg
     4801 gaaggtgttc gggggtcatg atgtatcctg aaaaattaaa gtacgcggcg attgtaggga
     4861 ttgctcatca gatgtccaga tcttgatgaa ttcctatttg tgagctacgt ctggacagta
     4921 acttgttaca acctgtagca tccacttgcc ggtcctgtga gttaataggg aatccagtgc
     4981 gaatctggag ctgacgcgca gcggtaagga aaggtgcgat gattgcgtta tgcggacact
     5041 gccatccggt gggaagtcat catctcttag tatcttagat acccctccaa gcccgaagac
     5101 ctgccggcca acgtcgcatc tggttctcat catcgcgtaa tattgatgaa acctgcggca
     5161 tccttcttct attgtggatg ctttacaatg attaaaaaag cttcgctgct gacggcgtgt
     5221 tctgtcacag ccttttccgc ttgggcacag gataccagcc cggatactct cgtcgttact
     5281 gctaaccgtt ttcagcagcc agtaaatact gtccttgcac ctactgatat tgttacccgt
     5341 gaggatattc aacgctggca gtcgaaagac ctcaacgatg ttatgagtca tctccccggt
     5401 gtaaatatct ctcaaactgg tggaatgggg acgagttctt ctctttatgt tcgtggcact
     5461 gaatcgcgcc atgttttagt gttgattgac ggtgtgccga tggctcgtgc cggtatcgac
     5521 aatgcaattg atattagcca gttcccggta tcacttgtac agcgtgttga atatattcgt
     5581 gggccgcgtt ctgccgttta tggttctggt gccatcggag gtgtggtgaa cattattaca
     5641 atgagtaatg atgagaaagc tcagttcaac gcaggaatgg gtacaaatgg ttatcaagcc
     5701 tatgatgggg taataaataa acgttttggc gatacgatgg taacagcagc aggtgcttac
     5761 cagacgacca aaggttttaa cattcagcct ggctcaccat acagtggtga tagcgatcgc
     5821 gatggatatc gcaacaaact tttctggggg gggctgcagc ataagtttaa cgataacttc
     5881 tcggggttct tccgtggtta cagctacaca gctaataatg attacgacca gggctcgatg
     5941 tatggttctg ctactggcaa cgaagaagct cagaattaca accagtcctg ggatactggc
     6001 ctacgctata actccggcat ctattcgtct cagttaattg ctaactatca acgactcaag
     6061 aattataact acacaaataa tcttggtcgt tatgcgggag atgccaccct ggatgacatg
     6121 gaacagcgtt atattcagtg gggtaatagc attgaggtag ggcatggtgc aattagcggc
     6181 gggatcgatt ggaaacagga aaaactgacg tccagcagca ttacaaaatc agatgattac
     6241 gaacgcgata ccaccgggtt atacctgact ggacagcaac agatttccaa tgtgactttg
     6301 gaagcttctg gccgagaaga tcatgatgaa cagtttggct ggcatggtac ctggcagaca
     6361 gctgctggct gggagtttat tgatggctac caggcaacac tttcttacgg taccagtttt
     6421 ctttcgcctt ctcttggaca gcaatacggg gctgctcgct ttgcttcaat ttatggtcct
     6481 ggtattgcag ccaacccaaa tttgaagcca gaagaatcta aacagtggga gattggaatt
     6541 gagggggtaa ctgggactct ggattggcgg ctctcaggat atcgttatga aattcagaac
     6601 ctgattgatt ataaaagcat aaataatgtc aaccagtaca ttaatgtgaa atcagccacg
     6661 attaaagggc tggaatggac gggaaatatt acaaccggtc ctattgaaca tcgcttgacg
     6721 ttacaatatg ttaacccgcg cgatgatgaa acagataaag tgctgtaccg tcgggcaaaa
     6781 cagcaggtta agtacgagtt gactggacaa gcttatggtt tcgaatggga cgtttcttac
     6841 caatatatag gtcaacgata cgacaacgct tatgacgaga cctctggctc atcacataca
     6901 gttaaattag gcggtgtgag cttgtgggat cttgcggttg cgtatccggt cacctctaac
     6961 ctgacagttc gtggtaaaat agccaacctg ttcgacaaag attatgagac agtctatggc
     7021 taccaaactg caggacggga atacaccttg tctggcagct acaccttctg aaccacgtcc
     7081 caccgtgctg gtgtttgact ccggcgtcgg tgggttgtcg gtctatgacg agatccggca
     7141 tctcttaccg gatctccatt acatttatgc tttcgataac gtcgctttcc cgtatggcga
     7201 aaaaagtgaa gcgtttattg ttgagcgagt ggtggcaatt gtcaccgcgg tgcaagaacg
     7261 ttatcccctt gcgctggctg tggtcgcttg caacactgcc agtaccgttt cacttcctgc
     7321 attacgcgaa aagttcgact tcccggttgt tggtgtcgtg ccggcgatta aacctgctgc
     7381 acgtctgacg gcaaatggca ttgtcggatt actggcaacc cgcggaacag ttaaacgttc
     7441 ttatactcat gagctgatcg cgcgtttcgc taatgaatgc cagatagaaa tgctgggctc
     7501 ggcagagatg gttgagttgg ctgaagcgaa gctgcatggc gaagatgttt ctctggatgc
     7561 actaaaacgt atcctacgcc cgtggttaag aatgaaagag ccgccagata ccgttgtatt
     7621 gggttgcacc catttccctc tactacaaga agaactgtta caagtgctgc cagagggaac
     7681 ccggctggtg gattctggcg cagcgattgc tcgccgaacg gcctggttgt tagaacatga
     7741 agccccggat gcaaaatctg ccgatgcgaa tattgccttt tgtatggcaa tgacgccaga
     7801 agctgaacaa ttattgcccg ttttacagcg ttacggcttc gaaacgctcg aaaaactggc
     7861 agttttaggc tgatttggtt gaatgttgcg cggtcggaaa attattttaa atttcctctt
     7921 gtcaggccgg aataactccc tataatgcgc caccactgac acggaacaac ggcaaacacg
     7981 ccgccgggtc agcggggttc tcctgagaat ctcaacagag aaagcaaaaa taaatgcttg
     8041 actctgtagc gggaaagcgt attatgcaca ccccgcgccg ctgagaaaaa gcgaag
//
LOCUS       DAPGOF010000200         8079 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.201, whole genome shotgun sequence.
ACCESSION   DAPGOF010000200 DAPGOF010000000
VERSION     DAPGOF010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8079
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.201"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..228
                     /locus_tag="RHV13_004124"
     CDS             <1..228
                     /locus_tag="RHV13_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243590.1"
                     /translation="DGPVMVVSSQRLHDMLNPTKDTNWNSTYIYKSRHEMLPVNLTPE
                     TLFGSKSQDKHALFPIFTASWRANRIKNKGI"
     gene            451..948
                     /gene="ubiC"
                     /locus_tag="RHV13_004125"
     CDS             451..948
                     /gene="ubiC"
                     /locus_tag="RHV13_004125"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HDV1243591.1"
                     /translation="MSHPALTQLRALRYCKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            961..1833
                     /gene="ubiA"
                     /locus_tag="RHV13_004126"
     CDS             961..1833
                     /gene="ubiA"
                     /locus_tag="RHV13_004126"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HDV1243592.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(1988..4411)
                     /gene="plsB"
                     /locus_tag="RHV13_004127"
     CDS             complement(1988..4411)
                     /gene="plsB"
                     /locus_tag="RHV13_004127"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HDV1243593.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4582..4950
                     /gene="dgkA"
                     /locus_tag="RHV13_004128"
     CDS             4582..4950
                     /gene="dgkA"
                     /locus_tag="RHV13_004128"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDV1243594.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5060..5668
                     /gene="lexA"
                     /locus_tag="RHV13_004129"
     CDS             5060..5668
                     /gene="lexA"
                     /locus_tag="RHV13_004129"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HDV1243595.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5741..7066
                     /gene="dinF"
                     /locus_tag="RHV13_004130"
     CDS             5741..7066
                     /gene="dinF"
                     /locus_tag="RHV13_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HDV1243596.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMYIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7182..7391
                     /locus_tag="RHV13_004131"
     CDS             7182..7391
                     /locus_tag="RHV13_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDV1243597.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7433..7948)
                     /gene="zur"
                     /locus_tag="RHV13_004132"
     CDS             complement(7433..7948)
                     /gene="zur"
                     /locus_tag="RHV13_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HDV1243598.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         1805 a         1988 c         2231 g         2055 t
ORIGIN      
        1 gatgggccag ttatggtggt aagctcccag cgcttacatg atatgttgaa tcctacaaaa
       61 gataccaatt ggaattccac gtatatctac aaatccagac atgagatgtt gcctgttaat
      121 cttactccgg aaacactttt cggctctaaa tctcaggata aacatgcgct tttccccatt
      181 tttactgcga gttggcgagc taatcgtata aagaataaag gtatttaagt aaaggaaaac
      241 atcaccgttc ctggcatcct gggcggtgat gcccctatgg ttgccctctc cagcgcgggc
      301 atcggcaaag cgtaaggttc aacagcgttt taccacttca tgcgattgtt gcgtttttgt
      361 tgcgtattag atcacttaat ttgctttaca tctcccgtaa acacttttct gcgatacaat
      421 gcctttacgt tatgtaacgg agagttcggc atgtcacacc ccgcgttaac gcaactgcgt
      481 gcgctgcgct attgtaaaga gatccctgcc ctggatccgc aactgctcga ctggctgttg
      541 ctggaagatt ccatgacaaa acgttttgaa cagcagggaa aaacggtgag cgtgacgatg
      601 atccgcgaag ggtttgtcga gcagaatgaa atccccgaag aactgccgct gctgccgaaa
      661 gagtctcgtt actggttacg tgaaattttg ttatgtgccg atggtgaacc gtggcttgcc
      721 ggtcgtaccg tcgttcctgt gtcaacgtta agcgggccgg agctggcgtt acaaaaattg
      781 ggtaaaacgc cgttaggacg ctatctgttc acatcatcga cattaacccg ggactttatt
      841 gagataggcc gtgatgccgg actgtggggg cgacgttccc gcctgcgatt aagcggtaaa
      901 ccgctgttgc taacagaact gtttttaccg gcgtcaccgt tgtactaaga ggaaaaaaat
      961 atggagtgga gtctgacgca gaataagctg ctggcgtttc atcgcttaat gcgtacggat
     1021 aagccaattg gcgcgttact gctgctctgg ccaacattat gggcgttgtg ggtggcgaca
     1081 ccgggcgttc cccagctctg gatcctggcg gtgtttgtcg cgggtgtctg gctgatgcgc
     1141 gctgccggat gtgtggtgaa tgattatgct gaccgcaagt ttgatggtca tgttaagcgc
     1201 acggcgaacc gaccacttcc cagcggcgcg gtaacagaga aagaggcgcg cgcgctgttt
     1261 gtagtgctgg tactgatttc gtttttactg gtgctgacgc tgaatacgat gactattctg
     1321 ttgtcgattg ccgcgctggc gctggcgtgg gtatacccat ttatgaagcg gtatacccat
     1381 ctaccgcaag tggtgctggg cgcggcgttt ggctggtcga ttccaatggc ttttgccgct
     1441 gtgagtgagt cggtgccatt gagttgctgg ttaatgttcc tcgccaatat tctctgggcg
     1501 gtggcttacg acacgcagta tgcgatggtt gaccgcgatg atgatgtgaa gattggcatt
     1561 aaatccacgg caatcctgtt cggccaatac gataaattga ttattggtat tttgcagatt
     1621 ggcgtactgg cactgatggc gatcatcggt gagttaaatg gcttaggctg gggatattac
     1681 tggtcaattc tggtggctgg cgcgctgttt gtttatcaac aaaaactgat tgccaaccgc
     1741 gagcgtgaag cctgctttaa agcatttatg aataataact atgttggtct ggtactgttt
     1801 ttagggctgg caatgagtta ctggcatttc tgatgatgta aaaaagccgg atgatcatcc
     1861 ggctttcttc tgggttgcct gatgcgctgc gcttctcagg cctacacaac agatcgcaat
     1921 ttattgaatt tgcagattat ggaaggccgg ataaggcgtt tgcgccgcat ccggcaattc
     1981 tctctgatta cccttcgccc tgcgtcgcac tctcaatcgt caaacgcacg tctgatgtaa
     2041 tcaactccgc cagcaactga taaaccttca ttgtttctgc cggttcggca tcgccgctat
     2101 cgctgatata cccttcatca cgcagcgtca gcaccagaga actgaacacc gccttgtcaa
     2161 agaactccgg cgcgttgatg ccgtgcagca cggagagacg ttgcgcgacg gtgcggctct
     2221 ctttctccag cgtaccgcgg ttgatcgacg ggttggcgct caacagccag aaggtgatgg
     2281 cataacgttg cagcgtttcg cgcgcgcctg cggccagcag ctgtagcgtg cgagaatgcg
     2341 ccgggttgat atgcaactca tcatcttgca gggtaatcag cccctgacgt tgcatctcat
     2401 ttgccagcgc atcaataacg tccggcaact cgtcgcgatc ccagcgcagg aacagctccg
     2461 ctttcagcat tgggtaaagc acattgacgt gctccatcaa tacgtcgcgg gagatgtggc
     2521 gatgctgggt gacaattgcc gccatcagcg aaggcagcac caacatatgc gcaatgttgt
     2581 tgcgatagta ggtcatcagc accgcttgct cgcgcggcag aatgatgatg tcgccaatag
     2641 tgtctttctc aacttcaaac ttgttcattt gcagcgcgtg atctataagc tcgctggcgc
     2701 tggctgaagg aacggtagag tccgtggagt aaggtacgtt gcgcatcaga tccaggtagc
     2761 agttgagttg ctcggttaac tgctcgcggg tgagtgagcg ctgacgtgat gccagtagcg
     2821 cagtacagca caggttcatg gcgtttgccg cgcctgcgtt gttaatgcgt accatcagat
     2881 cggcagcaat attattgacc gtcggcgtta accatgccgg acgcactgct tcgatgggat
     2941 cgatagattc acgccagtct ggtacgtgct ggttaaggta ggtcatcaac ggcattggtt
     3001 caccgaaatt gacgtaaccc tggccgagat tacgcagctt gcttaaaccg cgcagcatct
     3061 gcggcaggct ctctttctct ttcgtcgcgc cgcgcagttc tttggcgtaa gtacccactt
     3121 ccatgacgtg ctcataaccg atatagatcg gaatcagcgt aatcggacgc gtgccgccac
     3181 gcagcatcgc ctgaatggtc atcgacagcg taccggtttt tggatccagc aaacgccccg
     3241 tacgggaacg accgccttct acgaagtact cgacggaata accacggctg aacagttcgc
     3301 cgagatactc acggaaaacg gtggaataga gcttattgcc tttaaacgta cggcgaataa
     3361 agaacgcacc cagacggcgg aaaatcggcc cggcaggcca gaaattcagg ttgatcccgg
     3421 cggcgatatg cggcggcacc agcccctggt gatacagcac gtaagaaagc agcaggtagt
     3481 ccatgtgact gcggtggcaa ggcacataca ctagctcatg accgtcgtgg gccaactggc
     3541 gaacgcgctc ggcgttatgg acgttgatac cctggtaaag tcggttccag gtgaagccca
     3601 gaatgcggtc agtcaggcgg atcatctcgt aagagaaatt cgccgcaatc tcttccatca
     3661 gcgcaatcgc gttctgctgc gctttttcat gggagatttt tttgctgcgc gcttcatctt
     3721 ctaccgcttt ggcaatggcg cgggaggcga gcagcttatt aaacagatcc tgacgagcag
     3781 gcagacgtgg acccacggca gccagacgtt gacgggcaaa gtgcatacgc gccacgcgcg
     3841 ccagtttctg cgcgatagtt ttatccgtgc cgtgttcatc cgccatacgg cgcagcgaaa
     3901 ctgacggcga gaaacgcaca aaactgtcgc gaccgagcca cagtacagcg aaaaatttct
     3961 gtacgccgtt aagcatacgc agcggcgggt tcacttcgcc tttttcacgc cccggcgcgc
     4021 gaccaaacat caccgatact ggcaccatct gcacatccag atttgggttg ctacggtgca
     4081 aatcgagata gtcgtggaac agcttaatag actcttcttt cggcgtgtaa taggtgaata
     4141 cacgcggccc gccgtgaatg aacacatagc gcggcagtag cgtgccgtcg atttccagcg
     4201 gctctaacgg gtcaggcagg tcatgtgcca gacactgggc gcgcaacgtc agcaaatctg
     4261 ctttcgagtt gtacggtaaa acgtacataa ttggacgcga ggtatccagc cccagttccg
     4321 gggcaggatc tgccggaata gacttgcttt ttaccaggat gcttaatggt aaattcagta
     4381 atttgtagta aattcgtggc cagccggaca taaacgatgt aaagcctctg gttaataatg
     4441 caaatgcgcg gcaaggatag cagaaagtca tgggaaattc tgtggtatcc gctcatgttt
     4501 cgcgcggcgt tacgcaaacc cgaatcatcg gatttaacgg tacactgata ttgacgctca
     4561 taatgtaaaa aggttctttc aatggccaat aataccactg gattcacccg aattatcaaa
     4621 gctgctggct attcctggaa aggtttacgc gctgcatgga tcaacgaagc ggcattccgt
     4681 caggaaggcg tagcggtatt gttggcggtg gtcatcgcct gctggctgga tgtggacgca
     4741 attacccgcg tgctgcttat cagctccgtg atgctggtga tgattgtgga aatcctcaat
     4801 agcgccatcg aagcagtggt tgaccgaatt ggctctgaat accatgagct ttccggacga
     4861 gctaaagata tgggatccgc cgcggtgctg attgcgatta tcgtcgccgt gattacctgg
     4921 tgcatcctgt tatggtcgca ttttggataa cccttccaga attcgataaa tctctggttt
     4981 attgtgcagt ttatggttcc aaaatcgcct tttgctgtat atactcacag cataactgta
     5041 tatacaccca gggggcggaa tgaaagcgtt aacggccagg caacaagagg tgtttgatct
     5101 catccgtgat cacatcagcc agacaggtat gccgccgacg cgtgcggaaa tcgcgcagcg
     5161 tttggggttc cgttccccaa acgcggctga agaacatctg aaggcgctgg cacgcaaagg
     5221 cgttattgaa attgtttccg gcgcatcacg cgggattcgt ctgttgcagg aagaggaaga
     5281 agggttgccg ctggtaggtc gtgtggctgc cggtgaaccg cttctggcgc agcagcatat
     5341 tgaaggtcat tatcaggtcg atccttcctt gttcaagccg aatgctgatt tcctgctgcg
     5401 cgtcagcggg atgtcgatga aagatatcgg cattatggat ggcgacttgc tggcagtgca
     5461 taaaactcag gatgtacgta acggtcaggt cgttgtcgca cgtattgatg acgaagttac
     5521 cgttaagcgc ctgaaaaaac agggcaataa agtcgaactg ttgccagaaa atagcgagtt
     5581 taaaccaatt gtcgttgacc ttcgtcagca gagcttcacc attgaagggc tggcggttgg
     5641 agttattcgc aacggcgact ggctgtaaca tatctctgag accgcgatgc cgcctggcgt
     5701 cgcggtttgt ttttcatctc tcttcatcag gcttgtctgc atggcattcc tcacttcatc
     5761 tgataaagca ctttggcatc tcgccttacc catgattttc tccaatatca ccgttccgtt
     5821 gctgggactg gtcgatacgg cggttattgg tcatcttgat agtccggttt atttgggcgg
     5881 cgtggcggtt ggtgcaacgg cgaccagctt tctctttatg ctgttgctgt ttttacgcat
     5941 gagcaccacc gggctgactg cgcaggctta tggtgccaaa aatcctcagg cattagcccg
     6001 tgcgctggtg caaccgttgc tgttggcgtt gggggctggg gcgttaattg cgctgttgcg
     6061 tacgccgatt atcgatctgg cgctgcatat tgttggcggt agtgaagcgg tcctggaaca
     6121 ggcgcggcgt tttcttgaaa tccgctggtt aagcgctccg gcgtcgctgg cgaatctggt
     6181 attactcggt tggttactcg gcgtgcaata tgcccgtgcg ccagtaattt tgttagtggt
     6241 cggcaatatc ctcaacattg tgctggatgt ctggctggtg atggggctgc atatgaacgt
     6301 gcagggcgcg gcgctggcga cggttattgc ggaatatgca acattgctga ttggtctgct
     6361 aatggtgcgt aaaatcctca aactacgcgg aatttccggc gaaatgctga aaactgcctg
     6421 gcgaggaaac ttccgtcgct tgctggcgct taaccgcgat atcatgctgc gctcgctgtt
     6481 gttacaactc tgtttcggcg cgatcaccgt acttggtgcg cgactgggga gtgacattat
     6541 cgctgtaaac gcggttctga tgacgctact cacctttacc gcctatgcgc tggatggttt
     6601 tgcctacgcg gttgaagcgc attccggtca ggcgtacggt gcgcgcgacg gtagccagtt
     6661 gctggatgtc tggcgggcag cgtgccgcca gtcggggatc gtagcgttgc tgttttcggt
     6721 ggtctatttg ctggcagggg aacacatcat tgcgttgctg acgtcgttaa cccagattca
     6781 gcagctggcc gaccgctatc ttatctggca ggtgattttg ccgttggttg gcgtctggtg
     6841 ttatctgctg gacggcatgt atataggcgc aacgcgtgcc gccgaaatgc gtaacagtat
     6901 ggcggtggcc gccgcaggtt ttgcgctgac gctccttacg ctgccgtggc tgggcaatca
     6961 tggtttgtgg ctggcattaa ccgtctttct ggcgttgcgc gggctttctc tggcggctat
     7021 ctggcggcgt cactggcgca acggtacctg gtttgccgca acgtgacggt taaaaattct
     7081 gaataaatat tcctaagcca aattgctgac tacacttaat ctcacgttca gaagaaaagt
     7141 gaacgtactc tcattcacaa cctaacgatg aggtcttgat tatgaataaa gatgaagccg
     7201 gcggtaactg gaaacagttt aaaggtaaag tgaaagagca atggggcaaa ctgaccgatg
     7261 acgatatgac gatcattgaa ggtaaacgtg atcaactggt cggtaaaatc caggaacgtt
     7321 atggttatca gaaagatcag gcagaaaaag aggtcgtgga ttgggaaacc cgcaatgaat
     7381 atcgctggta attaatccct cctgcccgac gtgtacaagg atgtacgccc tcttaacgcg
     7441 gtttcttttt cacctgcaca gagtgatcat gctggcactg ttcaggatga cgacacgctt
     7501 ccacttccac gcatgccgca cagagcccat gtgcttcaat cacattatgc cgcagggcaa
     7561 accccatttt tgccgccagc gtatgcatga tgtcttccac gccttctgca cattcttctt
     7621 tcaccgcgcc gcagcgatcg caaataaaca tagctgacgt atgggtgggc tggtcgaaca
     7681 gatggcagag cacataactg ttggtggatt ccaccttatg cacaaaacct tgctcaagca
     7741 gaaaatccag cgcgcgataa accgttggcg gcttggcttg cggttcagct tcgcgcagca
     7801 aatcaagcag atcataagcg ctgatagcgc catcctgcag actcatcagg cgcaacactt
     7861 ccaggcgctg tggggtcagg cgcacattac gctgcgcgca gattttttca gcctgcgcta
     7921 ataactcctg cgttgtggtc ttttccatcg ggcacctcaa agtgggggtt aagaatgtcc
     7981 ttactttacc atgttccagg aaaaacaccg agaaccctta atgttgccgt aatgttgatt
     8041 ttctgttttg tacgtaaggt gttgttttgc ctggtcgta
//
LOCUS       DAPGOF010000201         7977 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.202, whole genome shotgun sequence.
ACCESSION   DAPGOF010000201 DAPGOF010000000
VERSION     DAPGOF010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7977
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.202"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..307
                     /locus_tag="RHV13_004133"
     CDS             <1..307
                     /locus_tag="RHV13_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HDV1243599.1"
                     /translation="KMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVVNEDD
                     CKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVFAGSGLS"
     gene            573..2810
                     /gene="nrfB"
                     /locus_tag="RHV13_004134"
     CDS             573..2810
                     /gene="nrfB"
                     /locus_tag="RHV13_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HDV1243600.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKECEQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            2797..5769
                     /gene="nfrA"
                     /locus_tag="RHV13_004135"
     CDS             2797..5769
                     /gene="nfrA"
                     /locus_tag="RHV13_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HDV1243601.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSITTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAEALRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARISQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRFFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            5770..6660
                     /locus_tag="RHV13_004136"
     CDS             5770..6660
                     /locus_tag="RHV13_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HDV1243602.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAVQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAVPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEHVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            6843..7604
                     /gene="envY"
                     /locus_tag="RHV13_004137"
     CDS             6843..7604
                     /gene="envY"
                     /locus_tag="RHV13_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HDV1243603.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
BASE COUNT         1997 a         2068 c         2044 g         1868 t
ORIGIN      
        1 aaaaatgacg gtcagatatt atatcagttc tgctgattta accgcagaga agttcgccac
       61 agcaatccga aaccactggc acgtggagaa taagctgcac tggcgtctgg acgtggtagt
      121 gaatgaagac gactgcaaaa taagaagagg aaatgcagca gaattatttt cagggatacg
      181 gcacattgct attaatattt tgacgaatga taaggtattc aaggcagggt taagacgtaa
      241 gatgcgaaaa gcagccatgg acagaaacta tctggcgtca gtctttgcgg ggagcgggct
      301 ttcgtaatct tgccctgcct gctcacctcc aggcacttcc actacatcat gacctgatcg
      361 gtgccaccac tcctcttcac tatgacgtta acctcccctc cttgacacca cgcttttgtc
      421 tacattctac gcaaacggca acttttataa ccagtgtaaa aataacgtgc cgcaatagtc
      481 atacctatta atggtaaaaa gctgtcacaa ttcataaaaa accttaatat acgccaccct
      541 aaacataacc agcgttaatg taaggttttt gtgtggactg gcttcttgat gtttttgcta
      601 cctggctcta tggcttaaaa gtaatcgcga taacgttagc ggtcatcatg ttcatcagcg
      661 ggctggacga tttttttatt gatgtcgtct actgggtacg ccgcattaaa cgcaagttga
      721 gtgtttatcg ccgctacccg cgaatgagtt accgcgaact gtataaacca gatgaaaaac
      781 cgttagcgat tatggttccg gcgtggaatg aaacgggcgt catcggcaat atggccgagc
      841 tggcggcgac cacgctcgac tacgaaaact atcatatctt tgttggcacc taccccaacg
      901 acccggacac tcagcgtgat gttgacgaag tgtgcgctcg cttcccgaat gtgcataagg
      961 tagtctgcgc gcgtcctggc cccaccagca aagccgactg tctgaacaac gtgctggacg
     1021 ccatcaccca gtttgagcgt agcgccaatt tcgcttttgc tggttttatt ctgcatgacg
     1081 ccgaagatgt gatttcaccg atggaattgc gtctgttcaa ctatctggtc gagcgtaaag
     1141 atctgattca gatcccggtg tatccgtttg aacgcgaatg gacgcacttc accagcatga
     1201 cttacattga tgagttttca gagctgcatg gcaaagatgt tccggtgcgt gaagccctcg
     1261 ccggacaagt gcccagcgca ggcgtcggca cctgtttcag ccgccgcgcc gtgaccgctc
     1321 tgttagctga cggtgacggt attgctttcg acgtgcagag tctgactgaa gattacgaca
     1381 ttggcttccg cctgaaagaa aaaggtatga cggaaatttt tgtccgtttt ccggtggtgg
     1441 acgaagccaa agaatgcgag cagcgtaaat ttttacagca cgcacggacg tcaaacatga
     1501 tctgcgtgcg cgaatatttc cccgatacct tttcgactgc ggttcgacaa aaatctcgct
     1561 ggatcatcgg cattgttttc cagggcttta aaacccataa atggacctcc agcctgacgc
     1621 tgaactactt tctctggcgc gaccgcaaag gggcaatcag taactttgtc agcttcctcg
     1681 caatgctggt gatgatccag cttttgctgt tgctggcgta tgaaagtttg tggcccgatg
     1741 cctggcattt cctttctatt tttagcggca gcgcatggtt aatgaccctg ctgtggctaa
     1801 actttggctt gatggttaac cgcatcgtgc agcgggtgat tttcgtcact ggctactacg
     1861 gcctgacgca ggggctacta tctgtcctgc gtcttttctg gggcaacctg attaacttta
     1921 tggccaactg gcgcgcgtta aaacaggtac ttcaacacgg cgatccacgt cgcgtcgcgt
     1981 gggataaaac aacgcatgac ttccccagcg tcactggcga tacccgctcg ttgcgcccgt
     2041 taggtcaaat tctgctggaa aatcaggtca tcactgaaga acagctcgat acagcactgc
     2101 gtaatcgcgt cgaaggtcta cgcctgggcg gttcaatgct gatgcagggg ctgattagcg
     2161 ccgagcagct ggcacaggcg ctggcagagc aaaacggcgt ggcgtgggaa tccatcgatg
     2221 cctggcagat cccgtcctcg ctgattgccg aaatgccggc ctccgtggcg ctgcattatg
     2281 cggtactgcc gctgcgtctg gaaaatgatg agttaattgt cggcagtgaa gatggtattg
     2341 acccggtttc gctggcggcc ctgacgcgta aagtcggacg caaagtgcgt tacgtcattg
     2401 ttctgcgggg acaaattgtc acggggttac gtcactggta tgcacgccga cgcggtcacg
     2461 atccgcgggc aatgttgtac aatgcggttc agcatcagtg gctcacggaa cagcaggccg
     2521 gtgaaatctg gcggcaatat gtgccgcatc agttcctgtt cgccgaaata ctgaccacgc
     2581 tcggtcatat taatcgttca gcaattaacg tgttgttatt gcgccatgaa cgcagttctc
     2641 tgccgctcgg caagtttttg gtcaccgaag gcgttatcag ccaggaaacg ttggatcgcg
     2701 tcctgacaat tcaacgcgaa ttacaagttt cgatgcaatc actattactc aaagcaggtt
     2761 taaacacaga acaggttgcg caactggagt ccgaaaatga aggagaataa ccttaatcgc
     2821 gtcatcggat ggtctggttt actgctgacg tctttattga gtaccagcgc acttgcagac
     2881 aatatcggca ccagcgcaga agagctgggg ctgagcgatt atcgccattt tgttatttat
     2941 ccccgtctcg ataaggcgct gaaggcacag aaaaataacg acgaagcaac cgccatccgc
     3001 gaatttgaat atatacacca gcaggtgccg gataatattc cgctgacttt ataccttgcg
     3061 gaagcctatc gccattttgg tcatgatgac cgggcgcggc tgttgcttga ggatcaactg
     3121 aaacgtcacc caggagatgc ccgacttgag cgcagtctgg cggctattcc ggttgaagtg
     3181 aaaagcatta caactgttga agaactgctt gcccagcaaa aagcgtgcga tgctgcgccg
     3241 accctgcgtt gtcgcagtga agtcgggcag aatgccctgc ggctggcaca gttacctgtc
     3301 gccagagcgc aactgaacga tgcgacgttt gctgcatcgc cggaaggaaa aacgctgcga
     3361 accgatctgc tgcaacgggc aatctacctg aaacaatggt cccaggcaga tacgctatac
     3421 aatgaagcac gccagcagaa cacattaagc gcggcagaac gccgtcagtg gtttgacgtg
     3481 cttcttgccg ggcagctgga cgatcggatc ctggcactgc aatcacaggg gatcttcacc
     3541 gatcctcagt catatattac ttacgcgacc gcgctggctt atcgtggcga aaaagcacgc
     3601 ctccagcatt atctcattga aaataagccg ctgtttacca cggacgcaca agagaaaagt
     3661 tggctctatc tgttatctaa atacagcgcc aaccccgttc aggcattggc gaattatacg
     3721 gtgcagtttg ccgacaaccg ccagtatgtt gttggcgcga cgctaccggt gctgttaaaa
     3781 gaaggtcagt acgacgcagc gcaaaaactg ctcgccaccc tccccgccaa tgaaatgctt
     3841 gaggagcgtt atgctgtcag cgtggcgacc cgtaacaagg ctgaagctct gcgtctggca
     3901 cgcttgttgt atcagcaaga accggcaaat cttacccgcc tggatcaact aacctggcaa
     3961 ctgatgcaga acgagcagtc acgggaagct gccgatttat tgctgcaacg ctatcctttc
     4021 cagggcgatg cgcgtatcag ccagacttta atggcgcgac tggcgtctct gctggaaagt
     4081 catccttacc tggcaacgcc ggcgaaggtg gcgattttat cgaaaccctt accgctggcg
     4141 gagcaacgtc agtggcaaag tcagttgccg ggtattgcag ataattgccc ggcaatagtt
     4201 cgcttgctgg gcgatatgtc gccttcctac gatgccgccg cctggaaccg tctggcaaag
     4261 tgttatcggg acacgctacc cggtgtggcg ttgtatgcat ggcttcaggc cgaacaacga
     4321 caaccgaacg cctggcaaca tcgtgcggta gcctatcagg cgtatcaggt tgaggactac
     4381 gccaccgcac tggcggcctg gcagaaaatc agtcttcacg acatgagcaa tgaggatctg
     4441 cttgctgctg ccaataccgc ccaggcggca ggaaatggtg cggctcgcga tcgctggcta
     4501 caacaggcag aacaacgtgg actgggaagc aatgccctct actggtggct gcatgcgcaa
     4561 cgttacattc ctggtcagcc ggaactcgca ctgaacgatc tcacgcgctc aatcaatatt
     4621 gcgccttctg ccaacgctta cgttgcgcgg gcgacaattt atcgccaacg tcataatgtc
     4681 ccggccgcgg tgagtgattt gcgcgccgcg ctggaactgg aaccgaataa tagcaacacc
     4741 caggcagcgc ttggttacgc cttgtgggat agcggtgata tcgcacagtc gcgggaaatg
     4801 ctcgaacagg cgcataaagg gctaccggac gatccggcac tgatccgaca actggcctat
     4861 gtgaaccagc gtctggatga catgcctgcg acgcagcact acgcccggct ggtgattgat
     4921 gacattgata atcaggcgct gataacccca ctgaccccag agcaaaatca gcaacgcttc
     4981 aatttccgcc gtctgcatga ggaggtcggt cgccgctgga cgttcagttt cgattcttcc
     5041 atcggcttgc gttccggggc aatgagtacc gctaacaata atgttggcgg cgcagcgcca
     5101 gggaaaagct atcgtagcta cgggcaactg gaagccgaat accgcatcgg acgcaatatg
     5161 ctgctggaag gcgacctgct ctcagtttat agccgcttct ttgccgatac cggagaaaac
     5221 ggggtgatga tgccggtgaa aaatccgatg tccggcaccg gtctacgctg gaagccgctg
     5281 cgcgatcaga tctttttcct cgccgtcgaa cagcagttgc cgctgaacgg gcaaaatggc
     5341 gcatccgata ccatgctgcg cgccagcgcc tcattcttta atggcggcaa atacagcgac
     5401 gaatggcacc cgaacggttc aggctggttt gcccaaaacc tgtacctcga tgcggcgcaa
     5461 tatatccgcc aggatattca ggcgtggacg gcagattatc gcgtcagctg gcatcagaag
     5521 gtagctaacg gacagactat tgagccttac gctcacgttc aggacaacgg ctatcgtgat
     5581 aaaggcactc agggcgcgca gcttggcggt gtcggggtcc gctggaatat ttggaccggc
     5641 gagacgcact acgacgcctg gccgcacaaa gtcagtctcg gcgtcgagta tcaacatacc
     5701 tttaaggcga ttaatcaacg taacggagag cgcaacaacg cgtttctcac cattggagtg
     5761 cactggtaaa tgcgtaagtt cattttcgta ttgctgacac tgcttttggt cagccctttt
     5821 tcctttgcga tgaaaggtat tatctggcaa ccacaaaacc gtgatagtca ggttaccgat
     5881 acccagtggc aggggctgat gagtcagtta cgtttgcaag gcttcgatac cctggttttg
     5941 caatggaccc gttacggcga tgcatttacc cagccagaac agcgcacgtt attgtttaag
     6001 cgggccgcag ctgtgcaaca ggctggtctg aagctgattg tcgggctgaa cgccgatccg
     6061 gaatttttta tgcaccagaa acagtcgtcc gcggcgctgg aaagctatct taatcgcctg
     6121 ctggctgccg atctccagca agccagatta tggagtgccg tacctggcgt aacgccggat
     6181 ggctggtaca tcagcgctga aattgacgac ctgaactggc gcagcgaagc cgcccgtcag
     6241 cctttgctaa catggttaaa caacgcgcag cggctgatta gcgatgtttc agcaaaaccg
     6301 gtttatatca gtagtttttt cgccggaaac atgtcgccag atggctatcg ccaactgctg
     6361 gaacacgtta aagcaaccgg cgttaatgtc tgggtacagg atggcagcgg cgtggataaa
     6421 cttaccgctg aacagcgtga acgttatcta caggccagcg ccgattgcca aagctccgcc
     6481 ccagcaagcg gcattgttta tgaactgttt gtcgccggca aaggcaaaac ctttacagcg
     6541 aaaccgaaac cggacgcaga aattgcctcg ctgttagcga aacgttcctc ttgcggtaaa
     6601 gacactctct atttctctct gcgctatttg cccgtcgcgc acggcattct cgagtattaa
     6661 atctcctcca ggtaagtcgg gtacgacctg gcttacctct ttcgctctcg tcagaattcc
     6721 atcaagatta attgctaaaa agcgtgctaa agaaagaaga tcgttatcgg cgaacgggcc
     6781 gatcactcat gcaggaagca tagatccttt gagcggagtt tctgatatct gaggagtgcg
     6841 aaatgcaatt gagcagcagt gaaccttgcg tggtgatcct gaccgaaaaa gaggtagagg
     6901 taagcgtcaa taaccatgct acgtttaccc ttccgaaaaa ctacctggcc gccttcgcgt
     6961 gcaacaataa cgtcattgaa ctctcaacgt taaatcacgt attaatcacc cacatcaacc
     7021 gtaacatcat caacgattat ctgttgtttt taaataagaa cttaacctgt gtaaagccct
     7081 ggtcgcggct ggctaccccg gttatcgctt gtcatagccg tacaccggaa gtgttccggc
     7141 tagccgccaa ccacagcaag cagcaaccca gcaaaccctg cgaagcggag ttgacgcgcg
     7201 cattgctttt taccgtattg tctaactttc ttgagcaatc gcggtttatt gccctactga
     7261 tgtatatctt acgcagcagc gtccgcgaca gcgtctgccg cattattcaa agcgatattc
     7321 agcattactg gaatctgcga attgtcgcca gttcgctatg tttaagcccc agcctgctca
     7381 aaaagaaatt aaaaaacgaa aataccagct atagccagat tgtcacagag tgtcgtatgc
     7441 gttacgccgt acagatgtta ttgatggata acaaaaatat cactcaggtg gcgcaattat
     7501 gtggctatag cagcacgtcg tactttatct ctgtttttaa ggcgttttac ggcctgacac
     7561 cgttgaatta tctcgccaaa cagcgacaaa aagtgatgtg gtgaagggca aagcggaaac
     7621 ggataagacg ggcataaata aagaagaaat ggcgcgccct gcaggattcg aacctgcggc
     7681 ccacgactta gaagttccta gaacgacatt ttaagtcaac aacttaccgc gccatctctg
     7741 cgctcacacg tcccactacc tcaaaacatg taaagccttg caagccattg cgaggcctta
     7801 tgtgtctcag ttttgtccct cttttttgta ctaaaaaaca tagtaattga ggataaacct
     7861 catgctattt tcgcttatat gcctctaaag gcatggcact taaatagata aaagcaccac
     7921 aaaagcataa aaaaaccaca cagtaaaacc gcaatatgaa acaataacag ataatta
//
LOCUS       DAPGOF010000202         7959 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.203, whole genome shotgun sequence.
ACCESSION   DAPGOF010000202 DAPGOF010000000
VERSION     DAPGOF010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7959
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.203"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            172..531
                     /locus_tag="RHV13_004138"
     CDS             172..531
                     /locus_tag="RHV13_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HDV1243604.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(651..830)
                     /locus_tag="RHV13_004139"
     CDS             complement(651..830)
                     /locus_tag="RHV13_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HDV1243605.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            904..2265
                     /gene="pabB"
                     /locus_tag="RHV13_004140"
     CDS             904..2265
                     /gene="pabB"
                     /locus_tag="RHV13_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HDV1243606.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVAEPICTLTTFGKETVVSESEKRTTTTDDPLQVLQLVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2269..2847
                     /locus_tag="RHV13_004141"
     CDS             2269..2847
                     /locus_tag="RHV13_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HDV1243607.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRAIEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            3031..4395
                     /gene="sdaA"
                     /locus_tag="RHV13_004142"
     CDS             3031..4395
                     /gene="sdaA"
                     /locus_tag="RHV13_004142"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDV1243608.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGQ
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4526..6124
                     /locus_tag="RHV13_004143"
     CDS             4526..6124
                     /locus_tag="RHV13_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDV1243609.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTTDRVLIKGSPVLIQWTPAASSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(6128..7684)
                     /gene="yoaE"
                     /locus_tag="RHV13_004144"
     CDS             complement(6128..7684)
                     /gene="yoaE"
                     /locus_tag="RHV13_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HDV1243610.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
BASE COUNT         1964 a         2059 c         1993 g         1943 t
ORIGIN      
        1 gattagcaac tctggttttc tttcttagct gaacaatctg gttatagcgt gcggactgac
       61 cgttaatttt ctccatctta ctggataaca caccatgagc tgctaccttt aaaacacctg
      121 aatgtcaaca ggttaactcg tgccatatcg ttataatccc tttgatatta gatgcaaatt
      181 aaggtcatat atagccttat cgataacatg gttaatttta aggataagaa tatgcctgct
      241 gtaatagata aagccctgga tttcattggt gccatggatg tatcagcgcc aacaccaagt
      301 tcgatgaatg aaagcacggc gaagggaatc tttaaatatt taaaagaact gggagtaccc
      361 gcgagtgccg ctgatattac ggcgcgagct gaccaggaag gctggaaccc tgggttcacg
      421 gaaaaaatgg ttggatgggc aaaaaaaatg gagacaggtg aacgctctgt gattaaaaat
      481 cctgaatact tttcaacata tatgcaggaa gaactaaaag cactggtctg agttaaattt
      541 atatcagcat aaatgggtca ggacgctttt aatcacatat aaaaagcgtc ctttttctca
      601 caatcaaata atcatcggct tctataacga aatcgatacg gattagtgtg ttactcgtct
      661 tcatcctcaa aacgcgccac gattcgctcg ccggagtggt tggcacgcag ctcttctgcc
      721 accagcgcaa ttgcctggcc gctgctcatc ccttgtgcca tcagttcctg gatccgctcg
      781 acagcttttt gctgctgttc atgggtgagt gaaggtaaac ctgcaaacat tgttaactcc
      841 tgctaaattg ttggcgctaa ttatttcatg ctacccggca catagccagt agagtcagga
      901 ctgatgaaga cgttatctcc cgctgtgatt actttaccct ggcgtcagga cgccgctgaa
      961 ttttatttct cccgcttaag ccacctgccg tgggcgatgc ttttacactc cggctatgcc
     1021 gatcatccgt atagccgctt tgatattgtg gtcgccgagc cgatttgcac tttaaccact
     1081 ttcggtaaag aaaccgttgt tagtgaaagc gaaaaacgca caacgaccac tgatgacccg
     1141 ctacaggtgc tccagctggt gctggatcgc gcagacattc gcccaacgca taacgaagat
     1201 ttgccatttc agggcggcgc actggggttg tttggctacg atctgggccg ccgttttgag
     1261 tcactgccag aaattgcgga acaagatatc gttctgccgg atatggcagt gggtatctac
     1321 gattgggcgc tcattgtcga ccaccagcgt catacagttt ctttgctgag tcataatgat
     1381 gtcaatgccc gtcgggcctg gctggaaagc cagcaattct cgccgcagga agatttcacg
     1441 ctcacttccg actggcaatc caatatgacc cgcgagcagt acggcgaaaa atttcgccag
     1501 gtacaggaat atctgcacag cggtgattgc tatcaggtaa atctcgccca gcgttttcat
     1561 gcgacctatt ctggcgatga atggcaggca ttccttcagc ttaatcaggc caaccgcgcg
     1621 ccatttagcg cttttttacg tcttgaacag ggtgcaattt taagcctttc gccagagcgg
     1681 tttattcttt gtgataatag tgaaatccag acccgcccga ttaaaggcac gctaccacgc
     1741 ctgcccgatc ctcaggaaga tagcaaacaa gcagaaaaac tggcgaactc agcgaaagat
     1801 cgtgccgaaa atctgatgat tgtcgattta atgcgtaatg atatcggtcg tgttgccgta
     1861 gcaggttcgg taaaagtacc agagctgttc gtggtggaac ccttccctgc cgtgcatcat
     1921 ctggtcagca ccataacggc gcaactacca gaacagttac acgccagcga tctgctgcgc
     1981 gcagcttttc ctggtggctc aataaccggg gctccgaaag tacgggctat ggaaattatc
     2041 gacgaactgg aaccgcagcg acgtaatgcc tggtgcggca gcattggcta tttgagcttt
     2101 tgcggcaaca tggataccag cattactatc cgcacgctga ctgccattaa cggacaaata
     2161 tactgctctg cgggcggtgg aattgtcgcc gatagccagg aagaagcgga atatcaggaa
     2221 acttttgata aagttaataa gatattacgc caactggaga agtaagacgt ggaataccgt
     2281 agcctgacgc ttgatgattt tttatcgcgc tttcaacttt tgcgcccgca aattaaccgg
     2341 gaaaccctaa atcatcgtca ggctgccgtg ttaatcccca tcgtccgtcg accgcaaccg
     2401 gggttgttgc tgactcagcg ttcaattcat ctgcgtaaac acgctggaca agtggcattc
     2461 cctggaggtg cagtcgatga cacggacgca tcagttatcg ccgccgcgct gcgcgaagct
     2521 gaagaagaag tcgctatacc gccttccgcc gttgaagtta tcggcgtgct gccgcccgtc
     2581 gatagcgtaa ctggctatca ggtaacccca gtggtcggca ttatcccgcc cgatttgccg
     2641 tatcgcgcca ttgaagatga agtctcggcg gtgtttgaaa tgccgctcgc ccaggcatta
     2701 catctgggtc gttatcaccc tttagatatc taccgccgtg gcgattcaca tcgggtatgg
     2761 ctgtcctggt acgaacagta ttttgtatgg ggaatgaccg caggcataat tcgtgagctg
     2821 gcgctgcaaa ttggtgtgaa accctgacta tacttatctt tacatctaca aaacactact
     2881 tgagacaatc atcgcaatat tagttaaatc gcggtttttg attagtttaa ttcatgtgaa
     2941 tagttaagcc agtcgccgcg ttccctctta cactatgcgc tgttattagt tcgttactgg
     3001 aagtccagtc accttgtcag gagtattatc gtgattagtc tattcgacat gtttaaggtg
     3061 gggattggtc cctcatcttc ccataccgta gggcctatga aggcgggtaa acagttcgtc
     3121 gatgatctgg tcgaaaaagg cttactggat agcgttactc gcgttgccgt ggacgtttat
     3181 ggttcactgt cgctgacggg taaaggccac cacaccgata tcgccattat tatgggtctt
     3241 gcaggtaacg aacctgccac cgtggatatc gacagtattc ccggttttat tcgcgacgta
     3301 gaagagcgcg aacgtctgct gctggcacag ggacagcatg aagtggattt cccgcgcgac
     3361 aacgggatgc gttttcataa cggcaacctg ccgctgcatg aaaacggtat gcaaatccac
     3421 gcctataacg gcgatgaagt cgtctacagc aaaacttatt attccatcgg cggcggtttt
     3481 atcgtcgatg aagaacactt tggtcaggat gctgccaacg aagtgagcgt gccgtatccg
     3541 ttcaaatctg ccaccgaact gctcgcgtac tgtaatgaaa ccggctattc gctgtctggt
     3601 ctcgctatgc agaacgaact ggcgctgcac agcaagaaag agatcgacga gtatttcgcg
     3661 catgtctggc aaaccatgca ggcatgtatc gatcgcggga tgaacaccga aggtgtactg
     3721 ccaggcccgc tgcgcgtgcc gcgtcgtgcg tctgccctgc gccggatgct ggtttccagc
     3781 gataaactgt ctaacgatcc gatgaatgtc attgactggg taaacatgtt tgcgctggca
     3841 gttaacgaag aaaacgccgc cggcggtcgt gtggtaactg cgccaaccaa cggtgcctgc
     3901 ggtatcgttc cggcagtgct ggcttactat gaccacttta ttgaatcggt cagcccggac
     3961 atctataccc gttactttat ggcagcgggc gcgattggtg cattgtataa aatgaacgcc
     4021 tctatttccg gtgcggaagt tggttgccag ggcgaagtgg gtgttgcctg ttcaatggct
     4081 gctgcgggtc ttgcagaact gctgggcggt agcccggaac aggtttgcgt ggcggcggaa
     4141 attggcatgg aacacaacct tggtttaacc tgcgacccgg ttgcagggca ggttcaggtg
     4201 ccgtgcattg agcgtaatgc cattgcctct gtgaaggcga ttaacgccgc acggatggct
     4261 ctgcgccgca ccagtgcacc gcgcgtctcg ctggataagg tcatcgaaac gatgtacgaa
     4321 accggtaagg acatgaacgc caaataccgc gaaacctcac gcggtggtct ggcaatcaaa
     4381 gtccagtgtg actaatactt cttactcgcc catctgcaac ggatgggcga atttatcccc
     4441 gctttctcgt ctgctgtaat attccccact acacttccac tgttgcgtca ggcgtttgtc
     4501 gccatacgct tacagggtgg cccgcatgca aaaagcacaa cggatcatta aaacctatcg
     4561 ccgtaatcga atgattgttt gtacgatttg cgcactcgtt acgctcgctt cgaccctgag
     4621 cgtgcgattt atttcacagc gtaacttaaa tcaacaacgg gtagtacaat tcgccaatca
     4681 cgctgtagag gaattagata aagtactgct tcccctacag gcaggtagcg aagtcttgct
     4741 tccgctgatt ggtctgccct gctctgtcgc ccatttgcca ttacgtaaac aggcggcaaa
     4801 actccaaact gtgcgatcca ttggcctggt gcaagacggc acactttatt gctccagcat
     4861 ttttggttat cgcaatgtgc ccgtcgtgga cattctggct gaacttcctg caccgcaacc
     4921 acttttacgc ctgacgaccg accgtgtcct gattaaaggc agtccggttt tgattcaatg
     4981 gacgcctgca gcgagcagta gcaatgctgg ggtcatggag atgattaaca tcgacttact
     5041 gacggcaatg ctccttgagc cacaactgcc gcaaatcagt agcgccagcc tgacggtgga
     5101 caaacggcat ttgctctatg gtaatgggct ggtagattcc cttccacaac ctgaagacaa
     5161 tgaaaactat caggtttctt cgcaacgctt tccttttacc attaacgtta atggtccggg
     5221 ggctacggcg ctggcatggc actatcttcc aacacaatta ccgctggcgg tgctgctaag
     5281 tttactggtg ggctacatcg cctggctggc gaccgcttac cggatgagct tttcccgcga
     5341 aatcaatctg ggcctggcgc aacatgagtt cgaattgttc tgtcagcctt tgcttaatgc
     5401 gcgcagccag caatgtattg gtgtagagat tttgctgcgc tggaacaatc cgcgtcaggg
     5461 ctggatttca ccggatgtgt ttattcctat cgcggaagaa catcatttaa ttgtgccact
     5521 gacccgctat gtgatggcag aaaccattcg tcagcgccat gttttcccga tgagtagtca
     5581 gtttcatgtt ggcattaacg tcgcacccag ccattttcgc cgtggtgtgc tgataaaaga
     5641 tctcaatcag tactggttta gcgctcaccc gattcagcaa ctgatcctcg aaatcaccga
     5701 acgcgatgcc ttactggatg ttgattatcg gattgcccgc gagctacatc gtaaaaacgt
     5761 caaactggcg attgatgact tcggcaccgg caacagttca ttttcctggc ttgaaacatt
     5821 acgtcctgac gtgctgaaaa ttgataagtc atttaccgca gctataggtt ctgacgcggt
     5881 taactcgacg gtgaccgata tcatcatcgc gctggggcaa agactgaata ttgaactggt
     5941 ggcggagggc gtggaaacgc aagaacaggc gaagtatttg cgccgtcatg gcgtgcatat
     6001 tttgcaaggg tatttgtacg cacagccgat gccgctacgt gattttccca aatggctggc
     6061 gggcagccaa ccgccgcccg cccggcataa tggacatatc acgcccatta tgccgttacg
     6121 ttaacgctta ctcatcttca tcgtgcgccg gttgctcttt aacaatgcga accagatcaa
     6181 cacgataatc attggcttca atgatggtga tatgcagtgg ccctacatca atcacatcgc
     6241 ccacacgggg aatgtgacca tttgccgaga tcacgaggcc cgcgaccgtc gcgatatcat
     6301 cgtcatcggc aaggtgctca acatcaagcg cctgctgcaa ggcatgcaaa tctgtaccgc
     6361 cttttaccag ccagccgtca ccatcagtaa tgatttccgg cgtttcgtca gcgtccggga
     6421 attcacccgc aatggcttcc agcacatcca gcggcgtgac cagaccttgt accacaccaa
     6481 actcgttggt cacgataaca aagctcccgc gagcacgacg cagcacgccc aacaggttga
     6541 tcggatcgag ggtttccggg acgataatcg ccggagacgc cgaagcaatc gccgccacat
     6601 caacgccctc ttccagcgcc accagcagtt ctttagcacg tacgattccg atgatttcat
     6661 ccagttcacc gcgacatacc gggaacaggc tgtgcggtga ggagagcagc tgctcgcgga
     6721 tttcatctac cccgagatta gcgtcaaccc agcttatttc accgcgcggc gtcatgatcc
     6781 cgcgcagaga acgcgacgcc agcgtcagta cgccgttaat catgtaacgt tcttcttcgg
     6841 caaatgcacc ttccgggatc ggcatcggca tcgggttatc ggcatcgtgc tgaacattgg
     6901 cctgacgttt cccgcccatc aaacgcagga tggcatcggc agtacgcgct cgcagcggca
     6961 aagtcgactg gtgacgaata aagttgcgac gcgcaatctg gttaaacact tcgatgatga
     7021 tcgagaagcc aatcgcggca tacaggtaac ctttcggaat gtggaaaccg aaaccttctg
     7081 ccaccagact cagaccaatc attaacagga agctcagaca gagcaccacc accgtggggt
     7141 gctggttaac gaatcgcgtc agcggtttgg atgccagcaa cataaccgcc atcgcaatca
     7201 ctaccgccgc catcatcacc ggcagatggt taaccatccc tactgcagta attaccgcat
     7261 ccaacgagaa gacggcgtca aggatgacaa tctgtgtgac gaccacccag aaactggcgt
     7321 agcctttacc gtggccggaa tcatgatcgc ggttttccag ccgttcatgc agttcggttg
     7381 ttgctttgaa cagcaagaat atccccccga acaacataat caggtcgcgt ccggagaagg
     7441 agaaatccat gacggtaaat agcggtttgg tcagcgtgac catccatgaa atcagcgaca
     7501 gcagccccag acgcataatc agcgccagtg ataaccccag caaacgcgct ttatcgcgtt
     7561 gttttggcgg cagtttgtca gcaagaatgg cgatgaagac caggttatcg atacccagca
     7621 caatttcgag aacaacaagc gtgagtagcc ccgcccaaat tgaggggtcc attaagaatt
     7681 ccatgacaag ctcctgctta aggaatagct attcgacgcc agaaataatg caggcgtaac
     7741 gacaaaatgc aaacgaaagg tgcggcatag agtgccagaa aggcaggcgt taaaaggcct
     7801 gatgctgaaa tgacgtcggt gacgatccat actgcgggct actgccctat actccatggt
     7861 tgttaaacgg gagttaaaca tatcagagac gcctctgatt tggcaaagat ttaccttcct
     7921 ttgcaaacga atgtgacaag gatattttac ctttcgaaa
//
LOCUS       DAPGOF010000203         7947 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.204, whole genome shotgun sequence.
ACCESSION   DAPGOF010000203 DAPGOF010000000
VERSION     DAPGOF010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7947
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.204"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1118
                     /gene="ompN"
                     /locus_tag="RHV13_004145"
     CDS             <1..1118
                     /gene="ompN"
                     /locus_tag="RHV13_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HDV1243611.1"
                     /translation="ALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYFSDNSAK
                     DGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGLKFADYG
                     SFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFFGLVNGL
                     NFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAYTSSDRT
                     NDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVANKTQNFE
                     VTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATYYFNKNM
                     STYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            1259..1693
                     /gene="uspF"
                     /locus_tag="RHV13_004146"
     CDS             1259..1693
                     /gene="uspF"
                     /locus_tag="RHV13_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HDV1243612.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            1869..2804
                     /gene="ttcA"
                     /locus_tag="RHV13_004147"
     CDS             1869..2804
                     /gene="ttcA"
                     /locus_tag="RHV13_004147"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HDV1243613.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(2933..4306)
                     /gene="dbpA"
                     /locus_tag="RHV13_004148"
     CDS             complement(2933..4306)
                     /gene="dbpA"
                     /locus_tag="RHV13_004148"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HDV1243614.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(4784..5767)
                     /gene="zntB"
                     /locus_tag="RHV13_004149"
     CDS             complement(4784..5767)
                     /gene="zntB"
                     /locus_tag="RHV13_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HDV1243615.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPC
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            6022..7254
                     /gene="dgcM"
                     /locus_tag="RHV13_004150"
     CDS             6022..7254
                     /gene="dgcM"
                     /locus_tag="RHV13_004150"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HDV1243616.1"
                     /translation="MITHNFNTLNLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(7275..7838)
                     /gene="smrA"
                     /locus_tag="RHV13_004151"
     CDS             complement(7275..7838)
                     /gene="smrA"
                     /locus_tag="RHV13_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HDV1243617.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
BASE COUNT         2091 a         2001 c         1880 g         1975 t
ORIGIN      
        1 tggcactttt aattcctgcc ctgctcgccg caggtgctgc acatgcagcc gaagtttata
       61 ataaagacgg caacaaatta gatctgtatg gcaaagttga tggcctgcat tatttttctg
      121 ataattcagc gaaagatggc gaccagagct atgcgcgtct gggttttaaa ggcgaaaccc
      181 aaattaacga tcaacttact ggctacggtc aatgggaata caatattcag gcaaacaaca
      241 ctgaatcttc aaaaaaccag tcatggaccc gtctggcatt tgccggactg aaatttgcag
      301 attacggttc tttcgattac ggacgtaatt atggcgtaat gtacgacatc gaaggctgga
      361 ccgatatgct gcctgaattt ggcggtgact cttataccaa tgcagacaac tttatgactg
      421 gtcgagccaa tggcgtcgcg acttatcgta atactgattt cttcggtctg gtaaatggtc
      481 tgaacttcgc ggtgcagtat caaggtaaca acgaaggtgc cagtaatggt caggaaggca
      541 ccaacaacgg acgtgatgtt cgccatgaaa acggtgacgg ctggggtctt tccacaacat
      601 atgatttagg catgggcttt agcgctggtg cggcatacac ctcttctgac cgcaccaatg
      661 accaggttaa ccatactgcg gcgggtggtg ataaagcaga cgcgtggact gctgggctaa
      721 aatacgatgc taacaatatt tacctggcaa ccatgtattc agaaacgcgt aatatgaccc
      781 cgtttggcga cagcgattat gctgttgcaa acaaaaccca gaactttgaa gtcactgcac
      841 agtaccagtt tgattttggt ctgcgtccgg cagtctcttt cctgatgtct aaaggccgtg
      901 acctgcacgc tgccggtggt gcagacaacc cggcaggtgt tgatgataaa gatctggtta
      961 aatacgccga tgttggcgcg acttactatt tcaataaaaa catgtccact tatgttgact
     1021 ataaaatcaa cctgttggat gaagatgaca gtttctacgc tgccaacggc atctctaccg
     1081 atgatattgt cgctttaggt ctggtttatc agttctaaat cctcctgccc gctgttatgg
     1141 cgggcttttt ctgcttattc ttcctccttt gatctaaatt aaaaatgtga actccgtcat
     1201 tacacaaaaa gtgtcatctg gcgttacact ttatgcggat actaaaacag gaggttttat
     1261 gaacagaacg attcttgtcc ctatcgatat ttccgattca gaattaactc aacgcgtgat
     1321 tagccacgtt gaggcagagg caaagattga tgatgcagag gttcatttcc tgacggtaat
     1381 accttcactg ccctactatg cctctctggg tttagcgtat tccgcagaat taccggcaat
     1441 ggatgacctg aaagcggaag ccaaatcgca actggaagag atcattaaaa aatttaaact
     1501 gccaaccgac agagtgcatg tccatgttga ggaaggctcg ccaaaagacc gcattctgga
     1561 attggcgaag aagatccccg ctcatatgat catcattgct tcccatcgac cggatatcac
     1621 cacttatctg ctcggttcta acgccgcagc tgtagtgcgt cacgcagagt gctccgtgct
     1681 ggttgtgcgc tgacactaac gcccgcacat tgctgcgggc tttttgattc atttcgcaaa
     1741 tgtgctgaca ttttcccctc taatccgtac catacacgcc acagttttta tatcagattt
     1801 cttatgctgg gcgttccggc atgacgcata ctcttctgat gccatataac gaattgagtc
     1861 gcttttaaat gtcgcaaaat caagaaatta gtaagaaaga acaatacaac ctgaacaaat
     1921 tacaaaaacg tctgcgtcgt aacgtgggcg aagccattgc tgacttcaat atgattgaag
     1981 aaggcgatcg catcatggtt tgcctctccg ggggtaaaga cagctatacc atgctggaga
     2041 ttctgcgcaa tttgcagcaa agcgcgccaa tcaatttttc gctggtggct gttaacctcg
     2101 atcaaaagca accgggcttc ccggaacacg ttctgcccga gtatcttgaa aagctgggcg
     2161 ttgagtacaa gattgttgaa gagaatactt acggtatcgt gaaagagaag attccagagg
     2221 gcaaaaccac ttgctcactg tgttctcgcc ttcgtcgcgg tatcctttat cgtaccgcaa
     2281 cggaactggg ggcgacgaag atcgcgttgg gtcaccatcg tgacgatatc ctgcaaacgt
     2341 tgttcttaaa tatgttctac ggcggcaaga tgaaaggtat gcctccgaaa ctgatgagcg
     2401 atgatggcaa acatatcgtt attcgtccgc tggcctactg ccgcgagaaa gatattcagc
     2461 gatttgccga tgcaaaagcg ttcccgatta ttccgtgcaa cctgtgcggt tcacagccta
     2521 acctgcaacg tcaggtgatt gctgacatgt tgcgtgactg ggataaacgt tatccagggc
     2581 gtatcgagac gatgttcagc gcgatgcaga atgtggtgcc gtcgcatctg tgcgatacca
     2641 acctgttcga ttttaaaggc attacccacg gttctgaagt ggttaacggg ggtgatctgg
     2701 cgtttgatcg cgaagagatc ccactacaac cggcgggctg gcagccagaa gaagatgaaa
     2761 atcagttgga tgagttacga ctgaatgtgg ttgaagtgaa ataaccagga tagcgcccga
     2821 tgcgcaagcg tatcgggcta ctcttatgga ggccggataa gacgcggcca gcgtcgcatc
     2881 cggcaatccc gaataagatg tttactcttg cacccggcaa ttcaacattt cattatttta
     2941 ataaccgcac ccggcacgtt tttcctttaa tcttcccgcc ctgtaactgt ttccatgctt
     3001 tatgagcaac agcctgacgg accgcgacat agacatgcgc cggatgcacg gcgattttgc
     3061 caatatctgc gccatcaagc ccgatatctc ctgtcagtgc acctaataca tcacccgggc
     3121 gcattttggc ttttttcccg ccatcgatac acaacgttgc catttctgct tccagcggca
     3181 caatggaact attagctggc ggcgtttgcc agttaagttt tatctgcaac atgtcagaaa
     3241 tgatattggc ccgctgtgct tcttccggag cacagaaact gatcgccaga ccgctatttc
     3301 ctgcacgagc tgtacgaccg atgcgatgta catgaacttc agggtcccac gccagctcaa
     3361 agttcaccac cagctcaagc gatttaatat ccagaccacg cgcagcaaca tcagtagcaa
     3421 ccagtacacg ggcgctaccg ttagcaaaac gtaccagagt ctgatcgcga tcgcgttgct
     3481 ccagatcgcc gtgtaacgac aatgcacttt gccctacttc attcagcgcg tcgcagacag
     3541 cctggcaatc ttttttggta ttgcaaaaca ccacgcaaga ggatggctga tgcaagctta
     3601 ataaccgttg caacagagga attttgcctt tgctggatgt ctcataaaat tgttgttcaa
     3661 tgggtggcaa agcatctgtt gagtcaattt caatcgccaa aggatcgcgt tgtactcgtc
     3721 cgctgattgc ggcgatggct tccggccagg ttgccgaaaa cagaagcgtc tgtcgagatg
     3781 caggcgcaaa acggatgaca tcatcaatgg catcgctaaa tcccatatcc agcatgcggt
     3841 cggcctcatc catcaccaaa gtattcagcg catccagtga taccgtgcct ttttgcaagt
     3901 gatccagcaa acgacccggc gttgccacga taatatgcgg cgcatgttgc aacgaatcac
     3961 gctgcatacc gaacggttga ccaccgcaca acgtcaaaat tttggtattt ggcagaaaac
     4021 gcgccagccg acgcaattca cctgccacct gatccgccag ttcacgcgta ggacacagca
     4081 ctaaagcctg ggtttgaaat agcgacgcat caatttgctg taacaagccg aggccaaaag
     4141 ccgccgtttt gccgctgccg gttttcgcct gcacgcgaac atcttttccg gcaaggatcg
     4201 ccggaagcgc ggcggcctgc accggcgtca tggttaaata acccaactca ttaaggttcg
     4261 tgagttgggc gggaggcaaa acattcaggg tagaaaaagc ggtcacaatc tattctcgtg
     4321 gtcatcgacg caaagttagc aggcgcgtat cctcgcagat ctacgctcac gatgcgacaa
     4381 tttaatcggt tcttcatcgg gtggtgggtc aggcatgggt tgcgggcgag ggatcggatc
     4441 gggcactgga acaggatcgc caggaatcgg ttcagggaca ggaatttgca aataaataag
     4501 tgtcgtcata tttccctccg gtcattgggt ggactcttaa agggtagacg ctgataaata
     4561 acaggcaaaa aaaagccgac tcatcaaagt cggcgtcgta cgaatcaatt gtgctatgca
     4621 gtaattcaaa aaaggaagta agacaatatg gagcgcaacg cccatcgctt gacgttgcat
     4681 tcacctgcaa tggagatatt gccctgaatg ggtagagagt ttattgactt cgctcaaact
     4741 ttgcggcgtt tttgtataca gacagccgga aaaattgctt ttgttacaac catttactac
     4801 ggtgcaacca taaagcaaca ccaccaataa gaacaactaa cagaatacaa aaaattgaaa
     4861 atccgaattg ccacccgccg ccagggatcc caccaaggtt gacgccaaat aaccctgtca
     4921 gaaaggtact gggtaaaaag accattgcca tcaatgacat tgtataggta cgacgagcta
     4981 aattttcctg catcacctga gcgatttcat ccgccatcac accagtccgt gctatacagg
     5041 cgtcgatttc gtcaaggccg cgcccaaggc gatcggcaat atcctgcatc cgacggcgtt
     5101 ggtcatcgct catacacggc aaacgttcac tggcaagacg agcataaaca tcacgttgcg
     5161 gtgccatata gcgacgcatc acgattaatt gtttgcgcag cagagccagg aatccacgcg
     5221 gtggaatttg ctgatcaagg agattatctt caaggtcgat aattttatcg tgcagctgct
     5281 cgataaattc gctggaatga tcggtcaacg catcgcacac atccaccagc caccctccgc
     5341 aatcggtcgg acccgtgccc tcttccagat cgctcaccac atcgtccagc gccagcactt
     5401 tgcgttgtcg ggtcgaaaca attaaccgcc cgtccatata tacacgcatg gcgaccagtt
     5461 gatcggggcg ttcatcggtg ctgccgttta tacagcgcaa tgtaatcagc gtgccttcac
     5521 cgagacggct gactcggggc cgtgtgctct cgcccgccag cgcatcacgt acgttattgg
     5581 gaagcagcgg tgttgtcgcc agccattggg cgctatcatg gtgtacataa ttaaggtgga
     5641 gccagcaggg atgcgcttca tcaatcacat ctgtattttc cagcggttta acgccgcctc
     5701 taccatccag catccaggca aatactgcat ccgggacatt aacgtccgat cccttaatcg
     5761 cttccacagt gcctccatca tcaacgcatt attttgtagt ctagccttct ggccctgtta
     5821 cgcaacatct catcacccca ttaccctgaa atgattaata aaattctgtc taaattgaat
     5881 acaaaaagca aaatgctttt ccgtatacaa accgtgtgaa gtgttaaata gcgtctatca
     5941 ttatcagaat tatctgatca tatgacgtga ctttattgcg atcggatagc aacaaaaatt
     6001 gataaaaata atgggatctc aatgattacg cacaacttca ataccctgaa cttactcacc
     6061 agtcctgtct ggatcgtttc gccctttgag gaacagttaa tttatgccaa tagcgcggcg
     6121 cgactgttga tgcaagacct cacgtttagt cagctacgaa ccggtcccta ttccgtctcc
     6181 tcacaaaaag aactgccgaa atacctctcc gatctgcaaa accaacacga tattatcgaa
     6241 atcctcactg ttcagcgtaa agaagaggaa acagcattaa gctgtcggct tgttttgcga
     6301 gagctgacag aaacagaacc ggtgattatt ttcgaaggta tcgaagcgcc ggcaacgctg
     6361 ggtttaaaag ccagtcgctc ggcaaattat cagcgcaaaa aacaaggttt ttatgcgcgc
     6421 ttttttctga ctaactctgc accaatgttg ttgattgacc cgtcacgaga tggccaaatc
     6481 gtcgatgcta acctcgccgc gctcaatttc tatggttata accatgaaac gatgtgccag
     6541 aaacatacct gggaaataaa tatgctcggg cgtcgcgtca tgcctatcat gcatgaaatt
     6601 tcgcatttac ccggcggtca taaacctttg aattttgttc ataaactggc ggatggatct
     6661 actcgtcatg tgcagaccta tgccggaccg attgaaattt atggcgacaa gctcatgtta
     6721 tgtattgtgc atgatattac tgagcaaaaa cggctggagg agcagctgga acatgctgct
     6781 caccatgacg cgatgaccgg attactgaat cggcgacagt tttatcacat tacggaacca
     6841 ggccaaatgc agcatctcgc catcgctcag gattacagct tgttgctcat cgacaccgat
     6901 cgttttaaac acattaacga tctctatggg cattctaaag gtgatgaggt gttatgcgcc
     6961 ctcgcccgca ccctcgaaag ttgcgctcgc aaaggcgatt tggtgtttcg ttggggaggc
     7021 gaagagtttg tcttattgct accaagaacc ccactggata ccgcgctttc gctggctgaa
     7081 actatccgcg taagcgtggc aaaagtgagt atttcgggct taccacgctt taccgtcagc
     7141 attggtgtgg cgcatcacga aggaaatgaa agcatcgatg aactgtttaa acgcgttgat
     7201 gatgctttgt atcgggcgaa aaatgatggt cgcaaccgcg tgctggcggc ataagccgcg
     7261 gatgcgtctc gagatcaacg actgcgctta gcgtggcgct cccagttttc ttgcttcgcc
     7321 tgcgccgttt tacgtagtgc gacgtaacac gccccgctgc cgccatgatg cggtagcgcg
     7381 gtgcaatatg cctgaacatc atcaaattcg gtcagccagc gcgccacata gctgcggaca
     7441 atattggcat gcgatttatc atcccgccct ttaccatgaa taatcagcac gttacgcaaa
     7501 ccatccgcca gggcttgttg aataaaactg aacaccattt tgcggcactc ttccaccggc
     7561 tggcgcaaaa gattcaggct cgcctgttgc ggatatttac cactacgcag cttatccagc
     7621 accccatgtt gcaacccttc ccgccgaaac tccagcggct gacttagcgg gataatgtcg
     7681 agaaatccgg tagtgaggaa attatcaagc tgcagcgtgt cgatacgctg cggcgcacgt
     7741 tggttacgcg ttggatgcca gtggacatcg gtagcacgtt tcagcggctg gacatcttcc
     7801 atggcgtcaa gaaacagcga tttgtcgtca aggttcatgt tacatcctcc cgcaattaag
     7861 agcgcgatat gataaccaga ccgggtcggt ccaacaacgt attacccaaa attccagtaa
     7921 taagttccaa atattgccga tatttta
//
LOCUS       DAPGOF010000204         7938 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.205, whole genome shotgun sequence.
ACCESSION   DAPGOF010000204 DAPGOF010000000
VERSION     DAPGOF010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7938
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.205"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            418..1701
                     /gene="ydfJ"
                     /locus_tag="RHV13_004152"
                     /pseudo
     CDS             418..1701
                     /gene="ydfJ"
                     /locus_tag="RHV13_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
     gene            1791..3251
                     /locus_tag="RHV13_004153"
     CDS             1791..3251
                     /locus_tag="RHV13_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDV1243618.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQI
                     DGLETVLAAMCEPQIAIVSLTITEKGYLHSPATGQLMLDHPMVVTDVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(3287..3490)
                     /gene="ydfZ"
                     /locus_tag="RHV13_004154"
     CDS             complement(3287..3490)
                     /gene="ydfZ"
                     /locus_tag="RHV13_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HDV1243619.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(3667..4353)
                     /gene="ydfH"
                     /locus_tag="RHV13_004155"
     CDS             complement(3667..4353)
                     /gene="ydfH"
                     /locus_tag="RHV13_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HDV1243620.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(4442..5188)
                     /gene="ydfG"
                     /locus_tag="RHV13_004156"
     CDS             complement(4442..5188)
                     /gene="ydfG"
                     /locus_tag="RHV13_004156"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HDV1243621.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            5325..7370
                     /gene="dcp"
                     /locus_tag="RHV13_004157"
     CDS             5325..7370
                     /gene="dcp"
                     /locus_tag="RHV13_004157"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HDV1243622.1"
                     /translation="MTTINPFLVQSTLPYLAPHFDQIANHHYRPAFDEGIQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREQLFIAGWTRAEKSDANDTRAIIQRLVEIRAQQATLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(7415..7933)
                     /locus_tag="RHV13_004158"
     CDS             complement(7415..7933)
                     /locus_tag="RHV13_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HDV1243623.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLAIAV
                     RFALAQLLQLLL"
BASE COUNT         1992 a         1828 c         2021 g         2097 t
ORIGIN      
        1 ttttaagagt gcaaaagaga aagggacaac gttgctcgca ctctggatta aatatagggt
       61 gttgttaaac tgcgttgata cataattgta ccggatattg agtggtctga agtcccagtc
      121 attaagtaat cgtttcgtga tatgccgcag aaacgttgta tgcaataacg ttctgcggtt
      181 ggctgatgca ctttcgagag tgtattgaat aatttcaagc cgttattgtt tttacgcgta
      241 acaccatgaa aagactgctt atctgttggg gtgttttttg gggcatatat gggacagaaa
      301 taggccacag atagatagta agatcgaact caggatgctt ttgaaaaaat gtaactatct
      361 gaaaaaacct agaaagcgcc aaggaaacca cagaatgaga aaaaacatct gtgaattatg
      421 gatttccagt tatattcgct cggcgcagcg ttagtgtttc atgaaatatt ttttcctgaa
      481 tcatcaacgg caatggcgtt aattttggca atgggaacct acggtgcagg ttatgtggcg
      541 cgtattgtcg gagcatttat tttcggcaaa atgggcgaca gaatagggcg taaaaaagtg
      601 ctctttatta ccatcaccat gatggggatc tgtaccacct taattggtgt gttaccgacc
      661 tatgcacaga ttggtgtttt tgcccccatc ttgctggtga cgctgcgtat tattcagggg
      721 ttgggtgcag gtgcggaaat ttccggtgcc ggtacgatgc tggcggaata tgcgccaaaa
      781 ggtaagcgcg gaattatctc ctcatttgtg gctatgggaa ctaactgcgg aaccttaagc
      841 gcaacggcaa tctgggcctt tatgttcttc attctcagta aagaggaact gctggcgtgg
      901 ggatggcgta taccgttcct ggcgagcgtt gtcgtgatgg tctttgctat ctggttgcgt
      961 atgaatctga aagaaagccc ggtctttgag aaggttaatg acagcaacca accgacagca
     1021 aaacctgcac ctgctggtag catgttccag agcaaatcct tctggctggc aacagggctg
     1081 cgttttggtc aggcgggtaa ctccggttta attcagactt tccttgcagg ctatttagta
     1141 cagacgttat tgtttaacaa agcaattcca acagatgcat tgatgatcag ttcgattctc
     1201 ggctttatga ccattccgtt gcttggttgg ttatccgata aaattggtcg ccggatcccg
     1261 tatattatta tgaatacctc cgcgattgtg ctggcgtggc caatgctttc tatcatcgta
     1321 gataaaagct atgccccgag caccattatg gttgcactga ttgtgattca taactgtgcg
     1381 gtgctgggat tatttgctct ggaaaacatt accatggcag aaatgttcgg ctgtaaaaac
     1441 cgctttaccc ggatggctat ttcaaaagaa attggtggtc ttatcgcttc cggttttggt
     1501 cctatcctgg caggtatttt ctgcaccatg acggaatcct ggtatccaat cgccattatg
     1561 atcatggcat attcagtgat tggtttaatc tctgcactga aaatgccaga agtgaaagac
     1621 cgtgatttaa gtgcgctgga agacgctgcg gaagatcaac cgcgtgttgt aagagctgcg
     1681 caaccttcca gaagtctgta aatcctcaat cccttctctt accggagagg ggatttttta
     1741 ttcatataac aaaacatgta gcttgccata tttatattta aggaatagtt atgggaaata
     1801 atttgttatc agcaaaagcg acactccctg tttatgatcg taataacctg gccccaagaa
     1861 ttgttcattt aggctttggt gcatttcacc gtgcgcatca gggcgtgtat gccgatattc
     1921 ttgctacaga acatttcagt gactggggat attatgaggt caacttaatc ggcggcgaac
     1981 agcaaattgc cgatttacaa cagcaagata atctttatac cgttgcggaa atgtcggccg
     2041 atgcgtggac ggctcgcgtc gttggcgtcg ttaaaaaagc cttgcacgta cagatagatg
     2101 gcttagaaac cgtgttggct gcgatgtgtg aaccgcaaat cgcgattgtc tctctgacaa
     2161 tcaccgaaaa agggtattta cactctccgg cgaccggaca gttaatgctc gatcacccga
     2221 tggtcgttac cgacgtgcaa aatccccacc agccgaaaac agcaacaggg gtgattgttg
     2281 aggcgctggc tcgccgtaaa gcggcaggac ttcccgcatt taccgtcatg tcatgtgaca
     2341 acatgccaga aaacggtcat gttatgcgtg acgttgtcac ttcctacgcg caagccgttg
     2401 atgtaaaact ggcacaatgg atcgaagata acgtgacttt cccatcaaca atggtggacc
     2461 gtattgtgcc cgcagtgaca gaggatacgc tggcgaaaat cgaacaactt accggtgtgc
     2521 gcgatcctgc tggcgttgcc tgtgaacctt tccgccagtg ggttatcgaa gataactttg
     2581 tagccggacg tccggaatgg gaaaaagcgg gagcagaact ggttagcgat gtactgcctt
     2641 atgaagagat gaagttgcgc atgctcaacg gcagtcattc attcctggcg tatctgggtt
     2701 atcttgccgg atatcagcac attaatgact gtatggaaga tgaacattat cgtcatgcgg
     2761 cgtatggctt gatgttgcag gaacaagcgc cgacgctgaa agtgcagggc gttgatttgc
     2821 aagattacgc taaccgatta attgcacgct atagcaaccc ggcgttacgt catcgaacct
     2881 ggcagattgc gatggatggc agccagaaat tgccacagcg gatgttggat tctgttcgct
     2941 ggcatctggc gcatgacagc aagttcgatc tgctggcgct gggcgtcgcg ggttggatgc
     3001 gttatgtcgg tggtgttgat gaacagggaa atccgattga aatcagtgac ccactgttac
     3061 ctgttattca gaaggctgta caaagtagtg ctgaagggaa agcgcgcgtc cagtcattgc
     3121 tggcgattaa ggcgatcttt ggtgatgatt tgccagacaa tagtttgttt actgcaaaag
     3181 tgacggaagc gtacttgtct ttattagcgc atggcgcgaa agcgaccgtg gcgaaatatt
     3241 ccgtgaagta aaaaaagcaa tgccgccatc ggcggcattc acacgcttag ttcatgccga
     3301 gacgaatcag gtccagaggt gccagtttct cttcacaacc ttcaacaact acggtttttc
     3361 cgcggcggat ttgctcagcg gtcagacctt cagtatcaat cgacaggatc ttgccagtgt
     3421 gaccggtgcc gctaaccata acacggctgc cagtggtgat tgcgttacgg ttacgatcgt
     3481 aggtcgtcat aggtgttttc tcctttctga tttacagtcg aagcccacgt aacgggcgca
     3541 atataaatac tcctgtcgag tgaaatattt tttgtttttg atcaagctca catctttttt
     3601 gatgcatgca tgagccataa ggcgggggaa aagactctca ccccggatgg gatgagagga
     3661 aatgaattac tcttcgctaa accagtcgct attttcctgg cggatctggc gcacggattc
     3721 gctgatttcc tgcaaatgtt gcgtcattgc acgttctacc gcatcgccat cacgtttttg
     3781 cagggcagag aaaatatcaa gatgctggcg taacagcatt tctggtggag aaacgtggtc
     3841 gaaactcata tagcgcacgc gatcaacggt cgctttcagg ttctcaatgg tatcccacgc
     3901 cagttgacag tcggcaatct gcgtcaggag ttgatggaag ttgtcatcaa gttcaaaaaa
     3961 atcatccagt tgcttgcgct caatggcaat gcgttgctgg tgaagatttt gttccagttg
     4021 gtagcactgg ctttcggtaa tcatgctcgc cgcccgacgc gccaccgcgc actcaatggc
     4081 ctgacggata aaactgccat tgcgcacctg ggccatggaa attttgttga cgtagctgcc
     4141 acgctgcgga cgaatttgaa tcaggccgtt ttccgccagt ttaataaagg cttcacgaac
     4201 cggctggcgt gacacattga aacgaacaga aacttctttt tccgacaacg gtgtgcctgg
     4261 ggcaatcagg cagtggacaa tgtcgcgacg aaggatacga taaatctgct gattgacagg
     4321 ctgtgtggga ttaagttgcg tttcgacggt cattcgttct tgcttgtgag tgagttaact
     4381 gcatgagtct accacttttt tatgacaagc aataacccgg cagttacgcc gggtataaaa
     4441 attactgacg gtggacattc agcccggcat agctttgggt aaccggcatc atttccaggg
     4501 tattgatatt gacgtgagca ggcagcgttg acacccacca gacggcttcg ctgacatctt
     4561 ctggcgttaa tgcaacggta ttttgatagg ttttttccgc tttaccgtca tcgcctttaa
     4621 agcggacatt ggaaaactcg gtgccaccca ccagacccgg ttcgatgtcg gtgacgcgca
     4681 ccgccgtacc atgcagatcc gtacgcagat tcaggctaaa ctgacgaaca aacgctttcg
     4741 tcgcaccgta aacgttacca ccggcatatg gccagctacc tgccgttgag ccaatgttaa
     4801 taatatgacc atgattacgt tcaaccatac ccggtaagac ggcgcgcgtc atatatacca
     4861 ggcctttatt gttggtatca atcatcgttt cccagtcttc aacgctggct ttatgcgcag
     4921 gctccatgcc caacgccagg ccggcattat ttaccaggat atcaatattg ctccactcgg
     4981 cagggagcga tgccagcatc tcttcaatag cggcgcggtt gcgaacgtcc agttgggcga
     5041 tatacagatt atctcccagt tcgtctttta actcctgcaa ccgttcctgg cgacggccag
     5101 tggcgataac tttatgccct tgttgaataa aacgacgagt aatgcattca ccaaaacctg
     5161 ccgttgctcc agttactaaa acgatcatct cactgttcct caacgctttt gtgtgtcaat
     5221 aacatagcac gtgatgctta agagcgggta atactggttt gcaagattgc actccgttat
     5281 cagtaagcct acgctatgga acacctcgcg ttcaggagaa gaaaatgaca acaattaatc
     5341 ctttccttgt gcaaagcaca ctgccgtatc tggctcctca ttttgatcaa attgccaatc
     5401 atcactatcg cccggcattc gatgagggaa tacagcaaaa gcgggcagaa attgctgcca
     5461 tcgcgcttaa cccgcaaaca cctgatttca acaatactat tctggcactg gaacaaagcg
     5521 gagaattact tacccgcgtt accagcgtct tttttgcgat gactgcggcg cataccaatg
     5581 atgaattaca gcgtcttgac gagcagtttt ccgctgaact ggcggaactg gctaatgata
     5641 tctatctgaa cggtgaatta ttcgcgcggg tagatgctgt ctggcaacgc cgtgaatctc
     5701 tggggcttga tagtgaatcc atccgcctgg tggaggtgat tcatcaacgt tttgtccttg
     5761 ccggagccaa acttgcgcaa gctgataaag caaaattaaa agtactgaat acagaagctg
     5821 cgacactgac cagtcagttt aaccagcgat tactggcagc aaataaatcc ggcggtctgg
     5881 ttgtgaacga tatcgcgcag ctggcaggaa tgagtgagca agagattgcg ctggcggcag
     5941 aggcggctcg cgagaaaggt ctggataaca aatggctgat tccgctgctg aataccaccc
     6001 aacaaccggc gcttgccgaa atgcgcgatc gtgcgacgcg agaacaactg tttattgcgg
     6061 gctggacgcg agcggaaaaa agtgatgcca atgatacccg cgctatcatt caacgtctgg
     6121 tggagatccg tgcacaacag gctacattac tcggttttcc tcattatgcc gcatggaaaa
     6181 tcgccgatca gatggcaaaa acacctgaag cagcacttaa ctttatgcgg gaaattgttc
     6241 cagcggcgcg tcaacgtgcg agcgatgaat tagcctccat acaggcggtt atcgataagc
     6301 agcagggcgg gtttagcgcg cagccgtggg actgggcatt ttatgccgaa caggtccggc
     6361 gggagaaatt tgatcttgat gaggcgcagc tcaagccata ttttgaatta aacacggtgt
     6421 tgaatgaagg tgtattctgg accgcgaatc agctcttcgg tattaagttt gtcgaacgtt
     6481 ttgatattcc tgtctaccat cctgacgttc gtgtgtggga aatatttgat cataatggcg
     6541 tggggctggc gttattttac ggtgatttct tcgcccgtga ttcaaaaagc ggcggtgcat
     6601 ggatgggcaa ttttgttgag caatcaacgc ttaatgaaac gcatccggta atttataacg
     6661 tctgcaatta tcagaaacct gctgccggtg agcctgcgtt gttactctgg gatgatgtca
     6721 taaccttatt ccatgaattt ggtcatacac tgcacggcct ttttgcccgc cagcgttatg
     6781 ccacgctttc cggtaccaac acgccgcgtg attttgtcga atttccttcg caaatcaacg
     6841 aacactgggc aacgcatccg caggtattcg ctcgctacgc ccggcattat cagagcgggg
     6901 cagcaatgcc tgacgaactg caacagaaaa tgcgtaatgc cagcctgttc aacaaagggt
     6961 atgagatgag cgaactgctt agcgccgcac ttctcgatat gcgctggcat tgcctggaag
     7021 aaaacgaagc aatgcaggat gtcgatgatt tcgaattgcg ggcgctggtg gcggaaaata
     7081 tggatcttcc tgctataccg ccacgctatc gcagcagtta tttcgcccat atttttggtg
     7141 gcggatatgc cgcaggttat tacgcttatc tgtggacgca aatgttggcc gatgatggtt
     7201 atcagtggtt tgttgagcag ggcggattaa cgcgtgaaaa tgggctgcgt tttcgcgagg
     7261 cgatcctttc cagaggtaac agcgaagatc tggaacgcct gtatcgacaa tggcgcggta
     7321 aggcacctca gattatgccg atgctgcaac atcgtggctt gaacatataa acaccaccaa
     7381 tggctggact ggagatatcg ccagtccagc ctggttatag tagtaattgc agcagctggg
     7441 cgagggcaaa cctcaccgct atagctaata ccgtttcgca gtcgccagca aaatgcataa
     7501 ccgcagtgta gttctggcct ttaatatgcc acgcgaacca gacggtaccc gctggcgtac
     7561 catcttcacc gccctccggt ccgccgtagc cggtaatggc aatactgaca tcagcatccg
     7621 cacgctctat ggcaccggtt gccatttctg ctgccacttt ctcactcacc gcagaatatc
     7681 gttcaagaga ttgctggctt acgctgagga ttttcatctt tgcctgatcg gtgaaagtaa
     7741 caaagcctgc accgtaaaat ttgggtgtat cttcagctgc acacagggcg ctagccaact
     7801 taccgccggt gcatgattcg gcggtagtta agcgtaattg atccgcgata agtctttgac
     7861 tcaacgcgga tgtcaggttt tcaatagtgt cggtatctgc aagttgtaca attttatctt
     7921 tattaatgtt cactgttt
//
LOCUS       DAPGOF010000205         7905 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.206, whole genome shotgun sequence.
ACCESSION   DAPGOF010000205 DAPGOF010000000
VERSION     DAPGOF010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.206"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..181
                     /gene="yafQ"
                     /locus_tag="RHV13_004159"
     CDS             <1..181
                     /gene="yafQ"
                     /locus_tag="RHV13_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HDV1243624.1"
                     /translation="TLLINNTLPLPAVYKDHPLQGSWKGYRDAHVKPDWILIYKLTDK
                     LLRFERTGTHAALFG"
     gene            337..1077
                     /locus_tag="RHV13_004160"
     CDS             337..1077
                     /locus_tag="RHV13_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HDV1243625.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(1048..1815)
                     /locus_tag="RHV13_004161"
     CDS             complement(1048..1815)
                     /locus_tag="RHV13_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HDV1243626.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(2021..2599)
                     /gene="lpcA"
                     /locus_tag="RHV13_004162"
     CDS             complement(2021..2599)
                     /gene="lpcA"
                     /locus_tag="RHV13_004162"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HDV1243627.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            2839..5283
                     /gene="fadE"
                     /locus_tag="RHV13_004163"
     CDS             2839..5283
                     /gene="fadE"
                     /locus_tag="RHV13_004163"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HDV1243628.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEEMA
                     TKPVKLPEKVRKVEAA"
     gene            complement(5326..5772)
                     /gene="ivy"
                     /locus_tag="RHV13_004164"
     CDS             complement(5326..5772)
                     /gene="ivy"
                     /locus_tag="RHV13_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HDV1243629.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            5953..6720
                     /gene="yafV"
                     /locus_tag="RHV13_004165"
     CDS             5953..6720
                     /gene="yafV"
                     /locus_tag="RHV13_004165"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HDV1243630.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMAVLREYRDKFPAWRDADEFRL"
     gene            complement(6774..7148)
                     /locus_tag="RHV13_004166"
                     /pseudo
     CDS             complement(6774..7148)
                     /locus_tag="RHV13_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308629.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(7194..>7905)
                     /locus_tag="RHV13_004167"
     CDS             complement(7194..>7905)
                     /locus_tag="RHV13_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HDV1243631.1"
                     /translation="IRTDEKSNEITAIPELLNMLDIKGKIITTDAMGCQKDIAEKIQK
                     QGGDYLFAVKGNQGRLNKAFEEKFPLKELNNPEHDSYAISEKSHGREEIRLHIVCDVP
                     DELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMTVRYYISSADLTAEKFATANRNH
                     WHVENKLHWRLDVVMNEDDCRIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRK
                     AAMDRNYLASVLAGSGLS"
BASE COUNT         1855 a         2003 c         2038 g         2009 t
ORIGIN      
        1 gacgcttctt atcaataata ctttaccgct tccagctgtt tataaagacc acccgctgca
       61 aggttcatgg aaaggttatc gcgatgctca tgtcaaaccg gactggatcc tgatttacaa
      121 acttaccgat aaactcttac gatttgagag aactggaact cacgcggcgc tctttgggta
      181 aagcccgctt ccgatgaaat ttgtaaaccc tgcgccgaca ggcttgcgta aaactctttt
      241 ggtgataatg tgagccttcc aaatccagac tattccgatt tgggatccat tttgccgtcc
      301 tggcgtgtgt aaccgtttta tcaaggaata agcagtatgc gtaaaatcgc attaattctt
      361 gcgatgttgt tgatcccgtg cgtttcgttt gccggtttgc tgggcagcag tagctcgacc
      421 acgccagtga gcaaagagta taagcagcag ttgatgggat cccctgtcta catccagatc
      481 ttcaaggaag aacgtacgct cgatctctac gtcaaaatgg gcgagcaata tcaactgctc
      541 gacagctata aaatctgtaa atattccggc ggcttagggc caaaacagcg tcagggcgat
      601 ttcaaaagcc cggaagggtt ttatagtgtc cagcgtaatc agttaaaacc agacagccgt
      661 tactacaaag cgattaatat tggtttcccc aatgcctatg accgtgcgca tggttatgaa
      721 gggaaatacc tgatgatcca cggcgattgt gtttccatcg gctgctacgc aatgaccaat
      781 cagggtattg atgagatctt ccagttcgtt actggtgcgc tggtgtttgg tcagccgagc
      841 gtgcaagtga gtatttaccc gttccgcatg accgacgcca atatgaagcg ccataaatat
      901 tccaacttta aggacttctg ggagcaactg aagccgggtt acgactactt tgagcaaacc
      961 cgtaagccac cgaccgtttc tgtggtcaat ggccgttacg tggtcagcaa gccgttaagc
     1021 cacgaagtgg tgcagccaca actggcatca aactacacgc tccccgaggc aaaataagcg
     1081 ccattcgcct ggcataattt tttgccaggt ttcattgccc gtcagcggct gtgtcgcaat
     1141 caccgtgacc acatcattcg gtgtggtctg cgagctgaag tcgatttcca catcctgatc
     1201 cagcaacgtt gccacgccaa acggcgcgcg gcgggtgatc cagtgtaaat tagtcgagca
     1261 atacgccatc acatagcgcc cgtcagaaag cagcatgttg aaaacgccct tctgccgcag
     1321 ttcatccgcc agtgaggcga tatatttaaa taccgccgcc atgttgcccg gtgtgcgcgg
     1381 gtaacgctgc gttaatttat gcagcagcca gcagaaggct ttttcgctgt cggtttcgcc
     1441 taccgggcgg aagttgccgg tttccagtga tttgtagccc gtcagttgtc cgttatgggc
     1501 ataagtccag ttacgccccc ataactcgcg ggtaaacggg tgagtatttt ccagcgccac
     1561 ctcgccccga ttagcctgcc gaatatgagc caccaccgaa caggatttta tcgggtagtc
     1621 ctggacaagt ttggcgatgg gggaattaaa gctgggttgt ggatctttaa atgtgcgaca
     1681 gcctttacct tcgtaaaagg taatgcccca gccatcttta tgtggcccgg ttccaccacc
     1741 acgctgtaca agcccggtga aactaaagca gatatcggta gggacgttgg cgctcatccc
     1801 gagcaattcg cacatgggta acctccacaa cagcgggagc gcatggcccc cggcgaagat
     1861 tgatttaaga attttgaggt cggatgcggc gtgaacgcat tattcggcct acggttcgcg
     1921 tacagatttg taggcatgat aagacgcgtc agcgtcgcat caggcatcag cgcacaaatg
     1981 ccggatgcgg cgtaaacgtc ttatccggcc tacgccagac ttacttaacc atctcttttt
     2041 caattaactg gatcaggata tggatcactt taatatggat ctcctgaatg cggtcggcat
     2101 aaccaaagtg cggaacgcga atttcgatat ccgccgtgcc agccattttg ccgccgtctt
     2161 taccggtcag ggtgatcact ttcatcccct tctcacgcgc cgctgcgatc gctttgatca
     2221 cgtttgcaga gttaccggag gtggagatcc ccagcagtac atcgccttcg cgacctaccg
     2281 cttcaacgta gcgggagaaa atatcattga aaccgaaatc attaccgacg caggaaatat
     2341 gactaacgtc agaaatggca atcgccgggt agcccggacg gttttcacgg tagcgaccgg
     2401 tcaactcttc ggcaaagtgc atggcgtcgc aatgggaacc gccgttgccg caggaaagca
     2461 ctttgccacc ggctttaaag ctatctgcta acaggaccgc cgcgcgctga atggcgtgaa
     2521 tattggcgtc atcttttaaa aagttagcca gcgtttccgc cgcttcgttc agttcgttac
     2581 gaataagatc ctggtacatg aggatatcct tcagcataaa tgtaatagac aaaatgcagt
     2641 gtaccggata ccgccaaaag cgagaagtac gggcaggtgc tatgaccagg actttttgac
     2701 ctgaagtgcg gataaaaaca gcaacaatgt gagctttgct gtaattatat tgtaaacaca
     2761 ttgctaaatg tttttacatc cactacaacc atatcatcac aagtggtcag acctcctaca
     2821 agtaaggggc ttttcgttat gatgattttg agtattctcg ctacggttgt cctgctcggc
     2881 gcgttgttct atcaccgcgt gagcttattt atcagcagtc tgattttgct cgcctggaca
     2941 gccgccctcg gcgttgctgg tctgtggtcg gcgtgggtac tggtgcctct ggccattatc
     3001 ctcgtgccat ttaactttgc gcctatgcgt aagtcgatga tttccgcgcc ggtatttcgc
     3061 ggtttccgta aggtgatgcc gccgatgtcg cgcactgaga aagaagcgat tgatgcgggc
     3121 accacctggt gggagggcga cttgttccag ggcaagccgg actggaaaaa gctgcataac
     3181 tatccgcagc cgcgcctgac ggctgaagag caagcgtttc tcgacggccc ggtagaagaa
     3241 gcctgccgga tggcgaatga tttccagatc acccatgaac tggcggatct gccgccagag
     3301 ttgtgggcgt accttaaaga gcatcgcttc ttcgcgatga tcatcaaaaa agagtacggc
     3361 gggctggagt tctcggctta tgcccagtct cgtgtgctgc aaaaactctc cggcgtgagc
     3421 gggatcctgg cgattaccgt cggcgtgccg aactcattag gcccgggcga gctgctgcaa
     3481 cattacggca ctgacgagca gaaagatcac tatctgccgc gtctggcgcg tggtcaggaa
     3541 atcccttgtt ttgcgctaac cagcccggaa gcgggttccg atgcgggcgc gattccggac
     3601 accgggattg tctgcatggg tgaatggcag ggccagcagg tgctgggcat gcgactgacc
     3661 tggaacaaac gctacattac gctggcaccg attgcgaccg tgctgggtct ggcgtttaaa
     3721 ctctccgacc cggaaaaatt actcggcggt gcagaagatt taggcattac ctgtgcgctg
     3781 atcccgacca ccacgccggg cgtggaaatt ggtcgtcgcc acttcccgct gaacgtaccg
     3841 ttccagaacg gaccgacgcg cggtaaagat gtcttcgtgc cgatcgatta catcatcggc
     3901 gggccgaaaa tggccgggca aggctggcgg atgctggtgg agtgcctctc ggtaggccgc
     3961 ggcatcacac tgccttccaa ctcaaccggc ggcgtgaaat cggtagcgct ggcaaccggc
     4021 gcgtatgctc acattcgccg tcagttcaaa atctctatcg gtaagatgga agggattgaa
     4081 gagccgctgg cgcgtattgc cgggaacgct tatgtaatgg atgctgcggc atcgctgatt
     4141 acctacggca ttatgctcgg cgaaaaacct gccgtgctgt cggctatcgt taagtatcac
     4201 tgtacccacc gcgggcagca gtcgattatt gatgcgatgg atattactgg tggcaaaggc
     4261 attatgctcg ggcaaagcaa cttcctggcg cgtgcttacc agggcgcacc gattgccatc
     4321 accgttgaag gggctaacat tctgacccgc agcatgatga tcttcggaca aggagcgatt
     4381 cgttgccatc cgtacgtgct ggaagagatg gaagcggcga agaacaatga cgtcaacgcg
     4441 ttcgataaac tgttgttcaa acatatcggt cacgtcggta gcaacaaagt tcgcagcttc
     4501 tggctgggcc tgacgcgcgg tttaaccagc agcacgccaa ccggcgatgc cactaaacgc
     4561 tactatcagc acctgaaccg cctgagcgcc aacctcgccc tgctttctga tgtctcgatg
     4621 gcagtgctgg gcggcagcct gaaacgtcgt gagcgtatct cggcacgtct gggggatatt
     4681 ttaagccagc tttacctcgc ctctgccgtt ctgaagcgtt atgacgacga aggccgtaat
     4741 gaagccgatc tgccgctggt gcactggggc gtacaggatg cgctgtatca ggcagaacag
     4801 gcgatggacg atttactgca aaacttcccg aaccgcgtgg ttgccgggct gctgaatgtg
     4861 gtgatcttcc cgaccggacg tcattatctg gcaccgtctg acaagctgga tcataaagtg
     4921 gcgaagattt tacaagtgcc gaacgccacc cgttcccgca ttggtcgcgg tcagtacctg
     4981 acgccgagcg agcataaccc ggttggttta ctggaagagg cgctggtgga tgtgattgcc
     5041 gccgacccga ttcatcagcg gatctgtaaa gagctgggta aaaacctgcc atttacccgt
     5101 ctggatgaac tggcgcacaa cgcgctggcg aaggggctga ttgataaaga tgaagccgct
     5161 attctggtga aagctgaaga aagccgtctg cgcagtatta acgttgatga ctttgatccg
     5221 gaagagatgg cgacgaagcc ggtaaagttg ccggagaaag tgcggaaagt tgaagccgcg
     5281 taattgaata acggagccga aaggctccgt ttctttatcc gctaattatt taaaattaaa
     5341 gccatccgga tggttttcca ggctgccggt caacgccgcg aacagcaccg ttttaccatc
     5401 aatcgaaagc gcatcgttca cattcaacca ggtgagtttc tcttgcgacg ttttctcatc
     5461 aatagtcgag aacagccccg tcatctgatt agatttctcg gaccacatca cagcgatacg
     5521 ttgcgagcca cagtcatgcg gtttgcacgc gctcatcacc tgatacgtct catctcccaa
     5581 cgttacggtt tgtgcagggg tataagtacc gcctttcatc acccaggcag gcagcttatg
     5641 cccttgcacc atctgattaa atgcagcttt ggtggtttcg ccctttgcaa ggctgctaat
     5701 ggttaaatca tcctgcgcca ttgcactggt ggcgatgacc agtgcggcga ctgtcgttat
     5761 tgccttaaac atcattcctc ccgagcttat cctgcccatg ttattaacct tctaaagcct
     5821 ggcgataaca cgataatttg caaatcagat atttccaata tcagcatttg ttatcaattt
     5881 attctgcgct aaggcaactg cctgataagg tgttaaagtt ataaaaccct tcgcataaag
     5941 gagttgtaca ccgtgcctgg tttgaagatt acgcttttgc agcaaccact ggtgtggatg
     6001 gatggccctg ccaacctgcg tcattttgat cgtcaactgg aaggtattac cgggcgcgat
     6061 gtgatcgttc taccggagat gtttaccagc ggctttgcca tggaagcggc agcttcgtcg
     6121 ctagcacaag atgacgtagt gaactggatg acagctaagg cgcagcagtg caatgcgctg
     6181 attgcaggca gtgttgcatt acaaacggag tctggttcgg ttaaccgctt tttgctggtt
     6241 gagccgggcg gcacggtaca tttttatgat aagcgtcatc tgttccgcat ggcagatgag
     6301 catctacatt ataaagcggg caatgcgcga gtgattgtgg aatggcgcgg ctggcgtatt
     6361 ttgccgctgg tgtgctacga cttacgtttt cctgtgtggt cgcgcaatct caacgattat
     6421 gacctcgccc tgtacgtcgc caactggcct gctccgcgct ctctgcactg gcaggcattg
     6481 ctgacggccc gcgcgattga gaatcaggcg tatgtggcgg gatgcaatcg cgtcggcagc
     6541 gatggcaacg gctgccatta tcgcggtgac agccgggtga ttaatccaca gggcgagatt
     6601 atcgctactg ccgacgcgca tcaggcaacg cgcattgatg cggagctgtc gatggcggtg
     6661 ttaagggaat atcgcgataa gtttccggca tggcgggatg cggatgagtt taggttgtag
     6721 tgagtgggtg ttgtgtttag ggctggggag agtcggggaa gactacgaaa gcccgctctc
     6781 ctgcgaccac ttttatcata ggagtgtgga agtgcttttc catcaatcgc gatgacgtct
     6841 ttgtcatttg atgaatggca gtcacgcatc cagttaataa agcactcgtg aaattttgca
     6901 ggactgatac aggatacaac tctggcaatg gtatcgtgaa caggaatacc attttcaaaa
     6961 tcaccatatt gcttcaaaaa atcgagatgt gtttccccaa aatcctctat atcttcccaa
     7021 ccttctgcac cagaaataac ggcacaaata gtcaacagta gaatatccga taatttatgt
     7081 tccacgttcc aggcttgtct gtaatcgggg ataacagaaa tatgttccat caatttttta
     7141 agttccattt tgttctcctt aattatgtaa ggagtatttg atccagggca agattacgaa
     7201 agcccgctcc ccgcaaggac tgacgccaga tagtttctgt ccatggctgc ttttcgcatc
     7261 ttacgtctta accctgcctt gaatacctta tcattcgtca aaatattaat agcaatgtgc
     7321 cgtatccctg aaaataattc cgctgcattt cctcttctta ttctgcagtc gtcttcattc
     7381 attaccacgt ccaggcgcca gtgcagctta ttttccacgt gccagtgatt tcggttcgct
     7441 gtggcgaact tctctgcagt taaatcagca gagctgatat aatatctgac cgtcatttcg
     7501 ggttctttct tttgttctgc tattattgac cgaaaggaga ctgccacgca taatttcttc
     7561 agccctttcc attcaaacgt gaaatcaata agttcatcag ggacatcgca aacaatatga
     7621 agacggattt cttctctgcc gtgactcttt tcactgattg cgtaactgtc atgctctgga
     7681 ttatttaatt ctttcagcgg aaatttttcc tcaaaggctt tatttagccg cccctggttt
     7741 ccttttaccg cgaataaata atcacctccc tgtttttgta tcttctctgc aatatctttc
     7801 tggcaaccca tcgcatcagt tgtgatgatt tttcctttaa tatccagcat gttaagaagt
     7861 tctgggatag ctgtaatctc attagatttc tcatccgtcc tgatc
//
LOCUS       DAPGOF010000206         7854 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.207, whole genome shotgun sequence.
ACCESSION   DAPGOF010000206 DAPGOF010000000
VERSION     DAPGOF010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.207"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            413..1369
                     /locus_tag="RHV13_004168"
     CDS             413..1369
                     /locus_tag="RHV13_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243632.1"
                     /translation="MTHSSDDKNYVRAVLSYLGIDFDEADIVLSVCHCQSDELSFTCN
                     IKAIELKNAVDLYVDSISENEIEALNRESLKSRLCYFLEVFDAVSGQYLEISGKHFAT
                     SRFEYDDVCSEVLSMSNDVSQSKGYDRDEYNRLMQVDGQVMIARFALQQFWDTHFIGL
                     ITFVSESITSSLYKAYETFSDISLACYKLSEYSYSRSINSELTLNISLKENDFCEDLS
                     DCYMEDEALPSGRVISRRNNDSIISIYESYAAASYIPMIASLEVVDEYGEVVTDLCHT
                     VYVSELTGGRIKIHDRNDLVSEVFDNLRNLNLAVGDSVSQAA"
     gene            1633..1908
                     /locus_tag="RHV13_004169"
     CDS             1633..1908
                     /locus_tag="RHV13_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243633.1"
                     /translation="MNELKSKNENSTKSNFPPVDNQFCFYHVDFSIERLLSTAEDLQL
                     EYIFQKPGSEVRKDLVERFERGERFVTASHCDNFCEILGCQGHQENA"
     gene            1953..2543
                     /locus_tag="RHV13_004170"
     CDS             1953..2543
                     /locus_tag="RHV13_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243634.1"
                     /translation="MIGESIFDWVIYGISILIFASALIEGKTTRYPALFFYGSLILML
                     ILIVAGGLNWWFYRSKYGAVIVIFTVVSLLMTRFRLVIYKLRLRKLVSEIRFRIKTGF
                     RIILVLSDNEDERNVLLSMLSNVLPEQTLIHTRDALGPHSGPILKALELHHQQGTGYI
                     LVCEQQISARTWLSIVENGKPDTSIAVNFHSIPEME"
     gene            2551..2808
                     /locus_tag="RHV13_004171"
     CDS             2551..2808
                     /locus_tag="RHV13_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243635.1"
                     /translation="MSRTVSQSVTEDCLFDTRTQVRLQLSRSVHKTLASAKEILQGRG
                     VDSVSIEDLVRACLEENQPIDLASLYLENLLKQKGTSICDF"
     gene            2882..3418
                     /locus_tag="RHV13_004172"
     CDS             2882..3418
                     /locus_tag="RHV13_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000107168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243636.1"
                     /translation="MSTQKQLSRTTGTERMFKEELALSLICWVFTSPFTNWTDKVFSG
                     IEVPSEGPSTLLGEAEKFFRFVVNEEGYDAGRAALGLDLCCFSLPSCLFPDAALSEEE
                     VMSKLTGEVIHGILLSLPEFIDMPEVLAYQVRDEILAFNTRCGDGIFHGWNTASELWK
                     FEIFPRTTIMMQHTSAIH"
     gene            3498..3881
                     /locus_tag="RHV13_004173"
     CDS             3498..3881
                     /locus_tag="RHV13_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243637.1"
                     /translation="MALWLTISFLMLSALLMTDHLLNKVILPPTWLLIWFSPMLGFLI
                     KVRVVETQHFLEHDLCVAGFSFYRWKRLVSPGLWSVDCINGRFRLNRITQVGKTIHYP
                     WRLHYHPLFMSPIEHKSHAVKAVSK"
     gene            3972..4934
                     /locus_tag="RHV13_004174"
     CDS             3972..4934
                     /locus_tag="RHV13_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HDV1243638.1"
                     /translation="MSYDDLPYFRDQILERIDSLKCFLSNTPPLMANLMTVSTVSRTE
                     ERLKQVKPIRVSVKDDASVEEIIQALTDICVDDIESLSHDSTKVTTKYPGLIIVPERA
                     DLLESLITSINEAKNDFAAAMRRIDNKKNVRFDKVHKKLPGLVAMHSTRNVLFIKSQL
                     KKVTFSWRLNRNQEVKTAEQLVSLLERRRASEVKNVATTNLNVVSNIDKALHRLEFHP
                     LKQGESYRLCRTNSFPVPIAHIFAFRPEGQERNGNKYAETDYSVVKASLPIFAAGNIP
                     QLKTLSDWAPENSQGPSNQRKLSLKYTELVPGAELGIFIVSPEN"
     gene            4944..5849
                     /locus_tag="RHV13_004175"
     CDS             4944..5849
                     /locus_tag="RHV13_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243639.1"
                     /translation="MQWISTASQKIDERLYRVCVWVKKYHADAINRVVLTVDLSKARH
                     DPDEDQVRAELLCGHYFLLRKENASNSAPDDQKFAFLPDGRNLCWQTATPVLQHLLLN
                     KNVPESLRLLTDYIHMRLARLTMVPMSGTIMNEALLDSISWVKVDLTYFWQYEQLSSH
                     LGPIQITHKALVRFGHLAKNDENSSAIRILRQRLSSEFLQEFQMAEDELKRKQSLMGT
                     MDVKMLFHAHYPSQKMLVARYKNGWVMVDCFLFHHTKPKKKAKNKTTVAKPQLKETKR
                     EGSENGTGHYGVHRVVTLRGGGVDQ"
     gene            5785..6129
                     /locus_tag="RHV13_004176"
     CDS             5785..6129
                     /locus_tag="RHV13_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243640.1"
                     /translation="MELGIMVCIVLLLSVAAAWISNKTVDLLERFYFKRPLSMEYAAW
                     LRVMCAGLLLVFIQVAPQFTLLYTLKLVTLINLGIQTMKLNGVYKYKRTGILPPGGAL
                     SNLTRLNPFKKK"
     gene            complement(6151..7326)
                     /locus_tag="RHV13_004177"
     CDS             complement(6151..7326)
                     /locus_tag="RHV13_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDV1243641.1"
                     /translation="MKNQSVIRQFSESELHQQLETFGNHDKQLSRLIRYFSHLRYNTA
                     KTYLHWLRVWNEWYLANARLHTDWPVSSLPVSEDALLAFMGHLEGKLSRSSINSCLQA
                     LNSIHKKGLNLPGIITSEAWYMLEALKQSEARKRKTTKQATPFLIGDLKALIKLRSTT
                     NSVRKLRDLCLIWTGFETLLRSSEIRRIRLKDLSLDSMTGEFNLTVYRTKTNISTLLT
                     YRLTRQLTNCLLRLMNLVKMDQHSHPDEFLFQAVNFHDTGYMPPGWKLRSKGNELSEL
                     LKRHNLPYRAKQSLLNDEDEEDTVDDAGMLSKNSLLRAFKEMWNELYPNETKTRYWTG
                     HSVRVGGAIQLNIEGYSLPQIMEMGNWSNEEMVMRYIRNIEAGKKAMIKLMRNAFDE"
     gene            7497..7709
                     /locus_tag="RHV13_004178"
     CDS             7497..7709
                     /locus_tag="RHV13_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243642.1"
                     /translation="MSDTKQSYLMKFRKCSSFETLEKVFERLCEKNSGVASLEISGAY
                     DHRKAELTMKKLYDKVPASVWTFVRE"
BASE COUNT         2179 a         1626 c         1703 g         2346 t
ORIGIN      
        1 tgggaacggg gcgttatatt gtgacaatga tgccctcata tccctctata ccgatgttgc
       61 ttcaattatc aatgtgccgg tgatgatgga agtaagattg atcaataaaa gagggcgtgt
      121 tgtctgtgat tcttcgcatt caacttacgt atctctcgaa agtaatgacc gatacagagt
      181 aactgatcgc acattactaa taactgaagc ttttgacgat tttcgtaacg cgtctcaatg
      241 atccttttgc ttcagtccaa aaaagtaact ttggttggct gaagacactt gtctatgaca
      301 atgtaaaatc tgtcgctggc atcttctttt agcgtaacca aatttttatc taacccagtg
      361 gggaagtcgc ttcccttctg gggcacttcc tctcaaattc gaggtagtgt atatgacaca
      421 ttcatctgat gataaaaact atgtccgagc agttctgagt tatcttggca tagattttga
      481 tgaggcggat atagtattaa gtgtttgcca ttgtcaaagt gacgagcttt cttttacctg
      541 taatatcaaa gctattgaac tcaagaatgc tgttgattta tatgtcgata gtatctctga
      601 gaatgaaata gaagctttaa acagagaatc tttaaaatcg cgactctgtt actttcttga
      661 ggtgtttgac gcagtctctg gtcagtacct tgaaatttca ggaaaacact ttgcaactag
      721 tcgttttgaa tatgatgatg tttgtagtga agttcttagc atgtctaatg atgtttctca
      781 gtcgaaaggt tatgaccggg atgaatataa cagactgatg caggttgatg gtcaggttat
      841 gattgcccgt tttgctttgc aacagttctg ggatactcat tttattggac taataacatt
      901 tgtatctgag tctataacat cgagcctcta taaagcttat gaaactttta gtgatattag
      961 tttggcttgc tataaactga gtgaatattc ttattcacgc tctatcaaca gtgaattgac
     1021 ccttaatatt tcacttaaag aaaatgactt ctgtgaggac ctatctgatt gctatatgga
     1081 agatgaggct ttaccttctg gtagggtaat ttctcgacgc aacaatgact ccataataag
     1141 tatttatgag agctatgctg cggcttctta tattccgatg attgcatctt tggaagtcgt
     1201 tgatgaatac ggcgaagtgg ttactgacct ctgccatacc gtttatgttt cagagttaac
     1261 cggtgggcgg attaaaatcc acgaccgtaa tgacttagta tctgaagttt tcgataacct
     1321 aagaaattta aatcttgcag ttggagattc agtttcacaa gctgcttgat tctttccctg
     1381 attttgattt tttctcacat actcttatgg cacggtttcc gtgccttttt tttgcctcgc
     1441 ttacaagcgt ctactcctct tctacctatc ccaccttaca tcaccactta aaatctatct
     1501 ttctaattcc attatccttt tcgtagcctc taatgctctg gtatgaacat actctttaaa
     1561 ctcaaaaaag tgctattaac gaaaaaaaat ccctaaagag atttgtatac tgttctcaac
     1621 caaggagagc tcatgaatga actaaaatcc aaaaacgaga acagtaccaa aagcaacttt
     1681 cccccggttg ataatcagtt ttgcttttac catgttgatt tcagcataga acgactactt
     1741 tctacggccg aggatctcca gcttgagtac atcttccaga aacctggaag tgaagtaaga
     1801 aaggatttgg ttgagcgatt cgaacgtgga gagcgctttg ttacagcttc acactgtgac
     1861 aatttctgtg aaatccttgg ttgtcaaggc catcaggaaa atgcataaac ccataaacct
     1921 acatttccac ttttaaggat gttcactaaa gaatgattgg agaaagtatt tttgactggg
     1981 taatttacgg aatatccatt ttgatatttg cctcagcact aattgaagga aagacaactc
     2041 gctacccagc actttttttc tatgggagtt taattttaat gttaatcctg atagttgctg
     2101 gaggccttaa ctggtggttc tacaggtcaa aatatggcgc agtaatcgtt atcttcacag
     2161 tggtttcttt actgatgact cgattccgcc tggtcatata taaattaaga ttaagaaagt
     2221 tagtttcaga gatcagattt cgtataaaaa cgggttttcg tataattctt gttttgtcgg
     2281 ataatgagga tgagcgtaat gttctgctgt ctatgcttag caacgtattg ccagagcaaa
     2341 cgcttattca cacgcgtgat gctctcggac ctcattcagg gcctatccta aaagctcttg
     2401 agctacatca tcaacaggga acaggttaca tactcgtctg tgaacaacaa atctcagcgc
     2461 gtacgtggtt gagtatcgta gaaaatggga aacctgatac atctatagct gttaattttc
     2521 attcgatccc agaaatggag tgatttttca atgagtagaa ccgtttcaca aagtgtcaca
     2581 gaagattgtc tttttgatac ccgcacacaa gtcagattac aactaagtcg aagcgttcat
     2641 aaaacgctgg catctgctaa agaaatttta cagggaaggg gggttgattc ggtctctatt
     2701 gaagatcttg tgcgagcctg tcttgaagag aaccagccaa ttgatctcgc ctcactttac
     2761 cttgaaaatc tcttaaaaca gaaaggtacc agcatttgcg atttttagtg ttttcaaacc
     2821 tattagataa aatgccggtt gtgtttatat aacatcaata atttcatgca acaggtaaaa
     2881 catgagtact caaaagcaac tttccagaac aaccggcact gaacgtatgt ttaaggaaga
     2941 gctggcgtta tccttaattt gttgggtttt caccagtcca tttaccaact ggaccgataa
     3001 agtcttcagt ggtattgaag ttccgtctga aggaccgtca acattactgg gtgaggctga
     3061 aaaattcttt cgttttgtcg ttaatgaaga aggctacgac gcaggaaggg ctgctcttgg
     3121 tcttgaccta tgttgttttt cacttccgtc gtgtttgttc ccggacgctg ccctgtctga
     3181 ggaagaggtt atgagtaagt taaccggaga agttatccat gggattcttc tttccttacc
     3241 tgaatttatt gatatgccag aagtgctcgc ttaccaggtc agggatgaaa ttcttgcttt
     3301 taacactcgc tgtggagacg ggatctttca tggttggaac acggcttcag aattgtggaa
     3361 gtttgaaatc tttccccgca ccaccatcat gatgcagcat acttcggcaa tacattaaaa
     3421 ttaggcgggg cagaatgcat aaaaaacttt ttcttctcga atggaaaccc tgtcagagcc
     3481 gcgtatgggc cggttggatg gccctgtggt tgactatcag ttttctgatg ttatctgcct
     3541 tgcttatgac agatcatctc ctgaacaaag tcatcctgcc gccaacatgg ttgttgatct
     3601 ggttttcgcc aatgctgggg tttttgataa aagttcgcgt cgtcgaaact caacatttcc
     3661 tggagcatga cctctgtgtt gcaggtttct ctttttaccg ctggaagagg ttagtttctc
     3721 ccgggctttg gtcagttgac tgtataaatg gtcgttttcg cctcaatcgg atcacccagg
     3781 tgggtaagac tatccactat ccatggcgtc tccattatca tcctttgttc atgagcccta
     3841 tcgaacataa atcgcatgct gtgaaagctg tttcaaaatg acttctttag aggtttacat
     3901 gacatttatg ttctgcaagg tcagccaggg cagcaaaatt gatttgaaat aacatgagcc
     3961 tggagaaacg tatgagttat gacgatttgc cctattttcg tgaccagatt ctggaacgta
     4021 tcgactcgct taagtgtttt ctttcgaaca cccccccgct gatggcaaac ctgatgactg
     4081 tctccaccgt atctcgaaca gaagagcgac tgaagcaggt taaacccatc agggttagtg
     4141 ttaaagacga tgcttcggtt gaggaaatta ttcaggcact tactgacatc tgtgtggatg
     4201 acatagagtc attaagtcat gattccacca aagtgaccac taaatacccg ggtcttatca
     4261 ttgtcccaga aagagcagat cttctcgaaa gcttgatcac atctataaat gaagcaaaaa
     4321 atgattttgc tgcggcaatg aggcgtatcg ataataaaaa gaatgtccgt tttgataaag
     4381 tgcacaagaa gctcccgggc cttgtcgcta tgcactcaac aagaaatgtc cttttcatca
     4441 agtcccagct taaaaaagtt actttttcat ggcggctaaa ccgaaaccag gaagttaaaa
     4501 cagcagagca acttgtgagt ttgttggagc gcaggagagc ctcagaagta aaaaatgtag
     4561 caactaccaa tttgaatgtc gtgtctaata tcgataaggc tttacaccgt cttgaatttc
     4621 acccactgaa gcagggcgaa tcttatcgcc tttgtcgaac taactctttc cctgttccga
     4681 ttgctcacat ttttgcattt aggcctgaag ggcaggaacg aaacgggaat aaatatgctg
     4741 agactgatta ttcagtagta aaagcatcct tgcccatttt cgcagcaggc aacatacctc
     4801 aattaaaaac gctctctgac tgggctcctg aaaacagcca gggcccgtcc aaccaacgga
     4861 agctgagcct gaaatataca gaactcgtgc cgggggctga gctgggcatt ttcattgtca
     4921 gccccgaaaa ttaaggaaca acaatgcaat ggatttccac agccagccag aaaatcgatg
     4981 agcgtctcta tcgtgtctgt gtctgggtaa aaaaatatca cgctgatgcg attaaccgcg
     5041 ttgtgcttac cgttgatcta agtaaagccc gacatgaccc tgacgaggac caggtaagag
     5101 cagagctgct gtgcggccat tattttttgc ttcgtaagga aaacgcttcg aactctgcgc
     5161 ctgacgatca aaagtttgct ttcctgcctg acgggcgcaa tctgtgttgg cagaccgcaa
     5221 ccccggtgct tcagcatctc ctgctcaata aaaacgttcc tgagtcgctg aggttgctta
     5281 cggactacat acatatgcgt ctggccaggc tgacaatggt accaatgtcc ggaaccatca
     5341 tgaacgaggc tctgctggac agcatcagct gggttaaggt ggatttgacc tacttctggc
     5401 agtacgaaca gctcagctct catctgggac ccatccagat aacccacaag gcactagtac
     5461 gcttcgggca tctggcaaag aatgatgaaa actccagtgc cataagaatc ctgcgtcagc
     5521 gtctgtcttc agaattctta caagaatttc agatggctga ggatgagcta aagcgcaaac
     5581 agtcgttgat gggcaccatg gacgtgaaaa tgctgtttca tgctcattac cccagccaga
     5641 agatgctggt ggctcgatat aaaaatggtt gggtaatggt ggattgtttt ctgtttcatc
     5701 ataccaaacc taagaaaaaa gcaaaaaaca aaactactgt ggctaagcct caacttaaag
     5761 agactaaaag agaagggagt gaaaatggaa ctgggcatta tggtgtgcat cgtgttgtta
     5821 ctctccgtgg cggcggcgtg gatcagtaat aagacagttg atttgctcga aagattctat
     5881 ttcaagcgtc cgctgagcat ggagtacgcg gcatggctac gtgtaatgtg tgcaggcttg
     5941 ttattggttt ttattcaggt tgctccacaa tttacacttc tgtataccct taaacttgtc
     6001 acgcttatta atcttggaat ccagacaatg aaattaaatg gcgtgtacaa atataaaaga
     6061 acgggaattt tgcctccggg aggggcactc tctaatctca ccagacttaa cccgttcaag
     6121 aaaaaataaa gttatgcgcc cgtgagggcg ctattcatca aaggcatttc gcataagctt
     6181 gatcattgct tttttaccag cttcaatatt acgaatgtag cgcataacca tttcttcatt
     6241 cgaccagttc cccatttcca taatctgtgg tagcgagtaa ccttcaatat ttagctgtat
     6301 cgctccgcct acacgaacgc tgtgccctgt ccagtaacga gttttagtct catttggata
     6361 aagctcattc cacatctctt taaatgctcg taaaagggaa tttttgctga gcataccggc
     6421 atcgtccaca gtgtcttctt catcttcatc gttcagaaga ctttgcttag cacgataggg
     6481 taggttatgc cttttaagaa gttcagatag ctcattcccc ttactccgaa gcttccagcc
     6541 gggtggcata tagcctgtat catggaaatt tacggcctgg aaaaggaatt catccggatg
     6601 actgtgctga tccattttca cgaggttcat caatctcaag aggcagttag ttaactgacg
     6661 tgtcagccgg taggtaagaa gtgtactaat gttcgttttt gtacggtata ccgtcaggtt
     6721 aaattcaccg gtcatactat ccagagacag atctttcaga cgtatacgtc gaatttcgga
     6781 tgaacggagc agtgtttcaa agcctgtcca gatcaggcac aggtcacgaa gtttacgaac
     6841 tgagtttgta gtactgcgta actttatcag agccttgaga tcgccaataa gaaagggagt
     6901 tgcctgttta gtcgtctttc gcttccgggc ttccgattgt tttaaggctt ccagcatgta
     6961 ccatgcttca gacgtaataa tcccgggcag gttaagccct tttttatgga tactatttaa
     7021 ggcctgaaga cagctgttta tgctgcttcg tgacaatttg ccttcaagat gccccataaa
     7081 cgcaagcaga gcgtcctcag acacggggag cgaacttact ggccagtctg tatgcaaccg
     7141 ggcattcgcc agataccatt cgttccatac tcgtagccag tgcagatagg ttttagcagt
     7201 gttgtatctc agatgggaaa agtaacggat caagcgtgaa agctgtttat catggttgcc
     7261 gaacgtttcc agctgctgat gcaattcgct ttccgaaaac tgtctgatca ctgattgatt
     7321 cttcatcctg ctcaccggga tacactggtt ttaattacag gtcattttag cactcatgta
     7381 cctggttatg aacagggtgg aaacaacgaa aaaggttgtg aaatcgttag gcttaagtga
     7441 aaaatgatat taagattatc cctggccagt tgaggcctgt ctaaagaagg ttgattatgt
     7501 ctgacacaaa gcagtcttat ttaatgaaat tccgtaagtg tagttcgttc gaaacgctcg
     7561 aaaaagtatt tgaaaggtta tgtgaaaaaa actcgggtgt cgcctctctt gaaatttctg
     7621 gtgcttatga tcatcgtaaa gccgaactga caatgaaaaa gttatatgac aaagttcctg
     7681 cgagtgtgtg gacctttgtt cgcgagtaat tattaatatt ggcacaacaa aaagactgtg
     7741 cctggatttg aaaatagttt atgccccttt cggggctaaa ggcattgtaa tagcctgtct
     7801 tttaaagttg tttattaagt tcggagaaaa actttaatca taagccaagc ccac
//
LOCUS       DAPGOF010000207         7824 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.208, whole genome shotgun sequence.
ACCESSION   DAPGOF010000207 DAPGOF010000000
VERSION     DAPGOF010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.208"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(521..1225)
                     /locus_tag="RHV13_004179"
     CDS             complement(521..1225)
                     /locus_tag="RHV13_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDV1243643.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(1362..1814)
                     /gene="moaE"
                     /locus_tag="RHV13_004180"
     CDS             complement(1362..1814)
                     /gene="moaE"
                     /locus_tag="RHV13_004180"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HDV1243644.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(1816..2061)
                     /gene="moaD"
                     /locus_tag="RHV13_004181"
     CDS             complement(1816..2061)
                     /gene="moaD"
                     /locus_tag="RHV13_004181"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HDV1243645.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(2054..2539)
                     /gene="moaC"
                     /locus_tag="RHV13_004182"
     CDS             complement(2054..2539)
                     /gene="moaC"
                     /locus_tag="RHV13_004182"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HDV1243646.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARVEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2542..3054)
                     /gene="moaB"
                     /locus_tag="RHV13_004183"
     CDS             complement(2542..3054)
                     /gene="moaB"
                     /locus_tag="RHV13_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HDV1243647.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(3076..4065)
                     /gene="moaA"
                     /locus_tag="RHV13_004184"
     CDS             complement(3076..4065)
                     /gene="moaA"
                     /locus_tag="RHV13_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HDV1243648.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDAQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(4053..4197)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4462..5370
                     /gene="yvcK"
                     /locus_tag="RHV13_004185"
     CDS             4462..5370
                     /gene="yvcK"
                     /locus_tag="RHV13_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HDV1243649.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDAHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHSALEKALQALG"
     gene            complement(5561..7582)
                     /gene="uvrB"
                     /locus_tag="RHV13_004186"
     CDS             complement(5561..7582)
                     /gene="uvrB"
                     /locus_tag="RHV13_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HDV1243650.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFQALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
BASE COUNT         1906 a         2078 c         1949 g         1891 t
ORIGIN      
        1 ctataaatgg cgtcaggtat accacgggga ggagaacaaa aagcatataa aagcggataa
       61 atcccaaacc aactccgtga cttgacggcc tgaaatatga cgtatatatc tcgtgaccga
      121 ggtcatgtgc catcaccata atcataacac ctgtaatcgc aataattatc tttatcactt
      181 tcataatttt atttcttcaa tatgcgtcca ttacctgcac cgtgaatgcc tgggccagac
      241 aagcacaaga gcgttcatgg caggaaaccg aaacaagcga ataaaatcgc aatagtgttt
      301 tatcccgcca gctaccagcc accctttgag gggccggttt tttccgggca agtaagtgac
      361 agcgaattga cggttcaaat gagttatcta tccctgaatt gacgcatacg gatagtgtgg
      421 catttgcagc gactgagttc tcatgaaaat gagcgaaaca gcctttatat aatccgaaaa
      481 aatcccggca ccaggccggg atcagcagga agaagagaat ttaacggcgg ttgccgaaga
      541 tccgcagcaa catcaggaac aggttgatga aatcgagata cagcgttaat gcgccaagaa
      601 tggaatattt gcgcaggttc gacgtgtcgc gggtatctat ctgctcaccc atatttttca
      661 gtttctgggt gtcatacgcc gtcaatccga caaagacaat cacgccgatg taggtgactg
      721 cccacatcaa cgcttcgctt ttcagccaga agttgaccag cgatgccaac acaatgccga
      781 ttaacgccat aaacagcata ttaccaaagc cgcttagatc gcgcttcgtg gtgtaaccat
      841 acaggctcat tgcgccgaac atcccggcag taacgacgaa agtactggcg atagaagcag
      901 cggtatagac aatgaaaata ctggaaagcg taagacccgt cagcgccgaa taaagcataa
      961 agagcatcgt cgttacacct gcgctcagct tttgaatcat cgctgataac acaataacca
     1021 atgctaattg cgcgatgatc agaccgatta aaaagacacg gttagtgaac aacagctcca
     1081 tcacggccgc ggaattagcc gcataccagg caacaaatgc ggtcagcaac aagccaacgg
     1141 tcatccagcc atagacttgc gccatataag tttgcaagcc agcccggggt tgtacgattg
     1201 aatcagaacg tgggaatctg tccatgacga tctcctgaag atataaggaa tatcttaagg
     1261 atactgcaaa atgatgaggc tgtgcatcga cgcagcgtaa acgcatgtac tgagcggtga
     1321 aattgccgga cgcagcggtg ccttatccgg ctaacaaaaa actaccagcg ttttgctgcc
     1381 tgctgatcgc tctcccgagc ttcaacccag cggtcgcctt caggcgtggc ttcgcgcttc
     1441 cagaacggtg cgcgggtttt gagataatcc ataataaact gcccggcttc aaacgcactg
     1501 ctgcgatgcg cactggtgac accgacaaaa acaatttcat cgcccggcca taattccccg
     1561 atgcggtgaa tcaccgtgac gcgccccagc ggccagcggt tacgcgcttc atcgacaatt
     1621 tctgccagtg ctttttcagt catccccgga tagtgttcga gggttaatgc tttgacgctg
     1681 tcgcccaggt tatggttgcg caccttacca gtaaaggtga ctaccgcacc gtcttcgtca
     1741 cgctccgcca gccacgggta ctcttctcct acgctgaacg gctgcggacc aacaacaatt
     1801 ttggtttctg ccatcttaac ctccggttac cggcgggaag aaagctactt cgtcgccgtc
     1861 agtcagcgga tggtcaaaac tcaccagcgt ctggttgacg gcagccagta atttgccatc
     1921 ttccagcgcc agcgcccagc gatcgctctg cgcagccagg tgctggcgta acgcttcaac
     1981 ggttgggaaa tccgcagcca cttcggttgc atctgttcct accaactcgc gcacctgggc
     2041 gaaaaaaaga actttaatca tccgcttcca ccttaaagtc acctgacttg ccgccgctct
     2101 tcgccagcaa acgtaccgga ccaatcacca tatctttttg caccgctttg cacatgtcat
     2161 aaatggtcag cgccgccacg gaggccgcgg ttaacgcttc catttcgaca ccggttttcc
     2221 cggtcaggcg gcataaggtt tctatacgca cccgattgtg ctccggctcg gcctgtaaat
     2281 tgacttcaac tttgctgagc atcagcggat gacacagcgg gatcagatcc caggtgcgtt
     2341 ttgccgcctg aataccggca atacgcgcag tggcaaatac gtcgcctttg tggtggcgac
     2401 catcaataat catcgccagc gtctcgctgc gcatggtgac aaaggcttcc acccgcgctt
     2461 cacgcacggt ttccgctttg gcggagacat ccaccatgtg cgcttcgcca gcggcgttga
     2521 tatgggtcag ttgcgacata cttatttctt caaatgtgga tggaaattac acggacgcgt
     2581 acgggcatcc agctgcggcg cgatgatatt ttcccatgcg gtacggcacg ctttggtcga
     2641 acccggcatg gcgaaaatca gcgttttgtt agcgacgccc gctaccgcac gagattgcaa
     2701 cgtggaagtg ccaatctctt caaacgacaa catacggaac acttcaccaa aaccttcaac
     2761 ttcacggtcg aacaacggca gcaatgcttc gggagcctga tcaccttccg tcaggccagt
     2821 cccccccgta atcaacacca cttgtacatc gtcgctggcg atccacgcag atacctgagc
     2881 gcgaatagcg tagcggtttt ctttcacaat ggctttatcg acaacgtgat ggcccgcttc
     2941 ttgcgccgaa tcgcgcaaat agtgaccgga ggtatcgtct tcttcaccgc gacgattaga
     3001 aaccgtaaga atagcaatac gggtcgggat aaattcagtg cttacctgac tcatctgatc
     3061 tctccttttg acgttttagc cgccaatgta cgataagttt tgcgtaatac cggtgttgtt
     3121 ttgatgcagg aaatgggtct gtttcttctc ccgcagcgcc gctgaaatac gcgcttccag
     3181 cgcctgttgc tgggcatcgt cttccagcag atcgcgcagg ttaacaccgc cttcaccaaa
     3241 caggcagaga tggagtttac caatggagga aacgcgcaga cggttgcaag tggcgcagaa
     3301 gtctttttca tacggcatga taaggccaat ctctccggcg taatccggat gacaaaagac
     3361 ttgcgcggga ccgtcgctgc gttgacgtaa ttggtggatc cagccgcgac gcagtagctc
     3421 gtcacgcaga acctgaccag agatgtgatg cttacggaag agctcgctgc cctcgcccgt
     3481 ttccatcagt tcgatgaaac gcagctggat gggacgatgc tggatccagt tcagaaaggt
     3541 gtcgagctgg tgatgattaa catcacgcat cagcactgta ttgactttga ccttctcaaa
     3601 accggcctca aatgcagcat caatccccgc catgacctgg ttgaatttat cctgcccggt
     3661 aatagcgtga aactggcggg cgtccagact gtcgacactg acgttgatgc cagtaagtcc
     3721 agcatcgcgc cagttcgcca catcgcgttc cagacggtaa ccattggtgg tgaccgcaat
     3781 ctggcggata gcgtcgtttt ctcgcacagc ggcgatgata tcggtaaagt cgcggcgtaa
     3841 agacggctca ccgcctgtca ggcgcacttt ttcggtgccc agactggcga aggcgcgcgt
     3901 aacccggcga atttcatcga cggtcagaaa gcctttattg gtgacgccgc tcggtttgta
     3961 gccatccggc aggcagtagg tgcaacgaaa gttacacaca tcggtaatcg acaggcgcaa
     4021 gtagtaaaac ttacgcgcaa atgcatcagt cagttgtgaa gccatgtaca cctttccaga
     4081 tacgggaggc gaagtcattt cttccttcgc cctggtggca atctttacga gcattgtcac
     4141 ggccaaagca ccgtatcagt tgacccaggt gcagaggcta gagtgtttag tggttaagcc
     4201 gatactagcg tgtaaacgag atttttacca tccacacttt cgctatgtaa tcatgtatat
     4261 agcgtcatga tcgtgctatt tcgctacaga ataagggaaa atggcggttt tactttgata
     4321 tacatcatca gggaggcata gcgtacatcg tcctttagag gtagaagaaa atagtttgta
     4381 tcctcaatat tggcaggtta attgctgttt ccccgcaatt tgcgctactg tagcgcggat
     4441 aatctgactc caggaaacta tatgcgcaat cgtacgctgg ctgatcttga tcgtgtcgtt
     4501 gctctcggcg gagggcatgg actgggacgc gttctctcat cactttcgtc tttgggttct
     4561 cgtttaacgg gtatcgtcac caccaccgat aacggtggct cgacggggcg tattcgccgt
     4621 tcagaaggcg gcattgcctg gggcgatatg cgcaactgcc ttaaccagct gataactgaa
     4681 cccagcgtcg cttccgcaat gtttgaatat cgtttcggcg gtaatggcga actttccggg
     4741 cataaccttg gaaacctgat gttgaaggca ctggatcacc ttagcgtgcg acctcttgaa
     4801 gcgatcaatc tgattcgaaa tctgctgaaa gtggacgcac atttgattcc gatgtcggag
     4861 catcctgttg atctgatggc gattgacgat caggggcatg aagtttacgg cgaggtcaat
     4921 atcgaccagt taactacgcc gattcaagag ttattgttaa cgcctaatgt acccgcaacg
     4981 cgtgaggcgg ttcacgctat caatgaagcg gatctcatca ttattgggcc tggcagtttt
     5041 tacaccagcc tgatgccaat tctgctgctg aaggaaatcg cccaggcatt acgccgcacg
     5101 ccagcgccga tggtctatat cggcaatctg gggcgtgagt tgagtttacc tgcggccaat
     5161 ttgaagctgg aaagcaagct ggcaattatg gagcagtatg ttggtaaaaa ggtcattgat
     5221 gcggtcatcg tcgggccaaa agtggatgtc tcggcggtga aagagcggat tgtgattcag
     5281 gaggtgctgg aggccagcga tatcccctat cgtcatgacc gtcagttgtt acatagcgcg
     5341 ctggaaaagg cattacaggc tttaggttaa cggaaatccg aaggaaaatt ccggcttcct
     5401 attgaagaca aagtgcgcgt tgtttatgcc ggatgcggcg tgaacgcctt atccggccta
     5461 caaaccgcgc taattcaatg tattgcagaa tcatgtaggc ctgataagcg tagcacatca
     5521 ggcaattttg cttttgtcat ctgtcttctt cgttatcctg tcacgaagcc gcgataaata
     5581 attcacgcag ctgatgcaac tggtcacgaa tttgtgccgc ttcttcgaac tccagattct
     5641 gcgcgtgttg catcattaac ccttccagct catggatttt ctgttgcaac gctttaggcg
     5701 acatatccat cggcacatta tccggctcaa caatcggtcg cgattttcct ctgcccttcg
     5761 ctttggtttt agcaatgttc tgccccaacg ccaggatatc gaccactttc ttgttcaagc
     5821 cttgcggcgt aatgccgtgt tcctcgttgt acttctgctg tttctcgcgg cgacgttcgg
     5881 tttcgccaat cgctttcgcc attgatgggg tgatcttatc gccgtagaga atcgctttac
     5941 cgttaacgtt acgtgccgca cgaccaatgg tctggatcaa cgaacgttcg gaacgcagga
     6001 agccttcttt gtcagcgtcg aggatcgcca ccagcgacac ttccggcata tccagacctt
     6061 cgcgcagtaa gttgatccct accagcacgt cgaactcacc cagacgcaag tcgcggatga
     6121 tttccatacg ttcgacggtg tcgatatctg agtgaagata acgcacgcgc tcgccgtgtt
     6181 cttcgagata ttccgtgaga tcttccgcca tccgcttggt cagagttgta accagtacgc
     6241 gttcgttaat tgccgctcgc tgacgaatct ccgaaagaag atcatcaacc tgtgtcgcca
     6301 ccggccgcac ttcgataatc gggtcaagca atccggttgg acgcaccacc tgatccacca
     6361 catcgccgcc ggatttttcc agctcataat tacctggcgt cgccgaaaca tagatggttt
     6421 gcggcgctaa tgcttggaac tcttcaaatt tcagcggacg gttatctagc gctgatggca
     6481 ggcggaagcc gtactccacc agtgtctctt tacgcgcccg gtcaccgcga tacatgccgc
     6541 caatttgtgg aatggtgacg tgagattcat cgaccaccag cagcccatcg gcagggaggt
     6601 aatcaaacag cgtcggcggt ggctcacccg gtccacgacc ggagaggaag cgcgagtagt
     6661 tttcaatccc cgaacagtag cccagctcgt tcatcatctc cagatcaaac tgggtacgct
     6721 gggtcagccg ctgctcttcc agcagtttgt tgttttccaa cagcactttg cgtctggcgg
     6781 ccagttcttc tttgatctcc tccatcgcct gtacgatgcg ctcacgtggc gtaacgtagt
     6841 gcgttttcgg gtagatggta aaacgtggaa tggtggaaac aatctgtccg gtcagcgggt
     6901 caaataacga caatcgttcc acttcctcgt caaacagttc cacgcgaagt gcaatgtcat
     6961 ccgattctgc cgggaagata tcaatcacct cgccccgaac acggaaagta ccacgctgga
     7021 atgcttgatc attacgagcg tattgcagct ccgccagtcg gcgcagaatc gcgcgctgat
     7081 cgataatcat accgaccgtg agatggagca tcatcttgag atataaatca ggatcgccca
     7141 gaccataaat cgcggaaaca gacgccacca caaccacatc acgccgctcc agcatcgctt
     7201 tggtggcgga caaacgcatc tgctcaatat gttcgttaac cgaggcatct ttctcaatga
     7261 aggtgtcgga actcggtaca taggcttccg gctgatagta gtcgtagtag gagacgaaat
     7321 actccaccgc gttttccggg aagaactctt tcatttcgcc atacagctgg gccgccagcg
     7381 ttttgttggg cgcaagtacc atggttgggc gctgaaggtc agcaatgaca ttggcaatgg
     7441 tgaaggtttt cccggagcca gtcacgccaa gtaacgtctg gtgcgccagg ccatcttcca
     7501 gcccctcttc gagacgtcga atcgcctctg gctgatcgcc agaaggttta aaagcggaat
     7561 tcagtttgaa cggtttactc atgagtcgct acctgaagga gttgggcggg caggtatgta
     7621 attttactcg tcgtacttaa ttatgccaac aaattatact ggataaaaaa acagttcatc
     7681 accataatat ttctgttaca gcgtaaactc cgctgtcaat catgagtaaa atttactctg
     7741 tggcgagata aaactccggc cttaccgggt tatccccaaa tcaacggctt ttttaacatt
     7801 tgtcaagatg agtgatgaca gttt
//
LOCUS       DAPGOF010000208         7812 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.209, whole genome shotgun sequence.
ACCESSION   DAPGOF010000208 DAPGOF010000000
VERSION     DAPGOF010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.209"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            255..773
                     /locus_tag="RHV13_004187"
     CDS             255..773
                     /locus_tag="RHV13_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HDV1243651.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            983..3124
                     /locus_tag="RHV13_004188"
     CDS             983..3124
                     /locus_tag="RHV13_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HDV1243652.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLALSLVSQQ
                     FLSLEFQQILDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQKLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIKSILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASDLHGEQPQAVPGR
                     RGSGTTLDNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAEKIGNNQAITVTNNQIQNIGVNQIQTVGVNQVETVGSNQIIKVGSNQV
                     EKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFS
                     VGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDAP
                     VINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            3200..7705
                     /locus_tag="RHV13_004189"
     CDS             3200..7705
                     /locus_tag="RHV13_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDV1243653.1"
                     /translation="MGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGIT
                     YANPVNPLLGAKVLPGETDLALPGPLPFILSRAYSSYRTRTPAPVGVFGPGWKAPFDI
                     RLQIHERELILNDNGGRSIHFEPLFPGEISYSRSESLWLARGGVLKQHKGHPLARLWR
                     ALPEAVRLSPHTYMMAVSTTGQWLVLSWPERVPEADEVLPPEPPAYRVLTGVVDGFGR
                     TLAFHRAAEGDVAGAVTGVTDGAGRRFHLALTTQAQRAEAFRKQRESSLSSPASPRSV
                     SSSSFSSAFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTASGEL
                     RAVYDRSGTQVRGFTYDVQHAGRMVAHHYAGRPESRYRYDDTGRVTEQVNPEGLDYRF
                     EYGQDRVTITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTDAA
                     GRRTEYRLHMASGKLTSVILPDGRTVRYGYNSQLQLTSVTYPDGLRSNRKYDRQGRLA
                     EETSRNGNITRWFYDSPRSSLPGAVEDGTGVTRRITRNRYGQLRAFTDCSGYTTRYEY
                     DRYGQQIAVHREEGISTYSSYNPRGQLVSQKDAQGREIRYEYSAAGDLTATISPDGKR
                     STIEYDKHGRPVSVTEGGLTRSMGYDAAGRITVLTNENGSQSTFRYDPVDRLTEQRGF
                     DGRTQRYHYDLTGKLTQSEDEGLITLWHYDASDRITHRTVNGDPAEQWQYDEHGWLTT
                     LSHTSEGHRVSVHYGYDDKGRLTGERQTVENPETGEMLWEHETGHAYSEQGLATRQEP
                     DGLPPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETARSFGGEAYELATAWNTSGQ
                     LRSRHLNLPQLDREYTWNDNGQLIRISGPQESREYRYSGTGRLTGVHTTAANLDIDIP
                     YATDPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYRHDEYGRLAEKTDRIPEGVIR
                     MHDERTHHYHYDSQHRLVFYTRIQHGEPQVESRYLYDPLGRRTGKRVWRRERDLTGWM
                     SLSRKPEVTWYGWDGDRLTTIQTGTTRIQTVYQPGSFTPLLRIETENGEQAKARHRSL
                     AEVLQEDTGVTLPAELSVMLGRLERELRAGAVSAESEAWLAQCGLTAEQMAAQLEAEY
                     IPERKLHLYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGETSAQHLQQSLRLPGQQ
                     YDEESGLYYNRNRYYDPLQGRYITQDPIGLKGGWNLYAYPLNPVQRGDPLGLSGETLV
                     LGSATTVTGTEFGGALLSGGAATFDAVTAAMASAAPALGAMAVVAGPMGYDLLANDGR
                     AGKALSDGQLINSQAEMCYWTSSCPLDSPHENDNSSGSQPNVGKDLTDEEKNCLAGGS
                     TGSPGGWGPEDENSARDRMQHERYKDELRQKMGKPDVTDSNLKNIIDDLYRPNAKVGS
                     GSTADAVRYELATGEKVGGRGHVLKAQDYTRALQDWLDKNPTASPGDRAAAENVLQDL
                     QNALGGK"
BASE COUNT         1846 a         1994 c         2494 g         1478 t
ORIGIN      
        1 cattttatgg aggcaatacg gactttgaac agttgggtat ggttatccag atatatatta
       61 ttaagttatt gctgaataag agatttaatg attttaaaaa tagtttttac gacaaccgta
      121 aattctagct cggtttgtat gtaaaaaata ctgaaaaatg atgcggttcc gtgaagtgag
      181 ctgcatctgc ctgtctggga ttaacggcag gtcattgtaa gtagtaatta accacgttaa
      241 caaggagttt acaaatgcca acaccgtgtt atatctccat cactggtcag acccagggaa
      301 atattactgc aggtgcattt accgctgatt ctgtcggcaa catctatgtg caggggcacg
      361 aagacgagat gctggtgcag gaattcctgc ataacgttac tgtcccgacc gatcctcagt
      421 ccggtcagcc tgcgggtcag cgtgctcaca agcctttcat ttttaccgtg gcgctgaaca
      481 aagcggttcc gctgatgtac aacgcactgg cctccggcga aatgctgcca accactgaac
      541 ttcactggtg gcgtacctct gtggaaggca aacaggagca ttatttcact acccgcctga
      601 ctgattcgac gattgtcgat atgaaactgc atatgccgca ttgccaggac ccggcgaaac
      661 gcgagttcac ccagttgctc gaagtttcac tggcctaccg caaaattgaa tgggaacacg
      721 tcaaatctgg cacctccggt gccgacgact ggcgcgcacc gctggaagca taagttaagc
      781 caacagcatc cggctatcag gccggatgct gtttttgtat tacgccgtag aaagaaaggt
      841 tgtcagtaag gggttacgtt cacgggagat ggcggctgcc ggaaacttcg gcggtagcgt
      901 gcctgaacgc aagcgtaaca ctgtactgtc acagccgctt taacagcgaa ctgtctgttt
      961 ttccttaacc ccggaggttg ctatgtcaac cggattacgt ttcacgctgg aagtggacgg
     1021 cctgccaccg gatgcttttg cggtggtctc ctttcatctg aaccagtcac tctcttcgct
     1081 tttttccctc gctctctctc tggtcagcca gcagtttctc tcccttgaat tccagcagat
     1141 cctcgacaaa atggcctacc tgacgatatg gcagggcgat gacgtacagc gccgggtgaa
     1201 aggtgtggtg acctggtttg aactggggga gaacgacaaa aaccagaagc tgtacagcat
     1261 gaaggtatgc ccgccgctgt ggcgcacagg gctgcgccag aacttccgta tcttccagaa
     1321 cgaggacatc aaaagcatcc tcggcacgat attgcaggaa aacggggtga ccgagtggag
     1381 tccgctgttc agtgaaccgc atccttcccg tgagttttgt gtccagtacg gtgagactga
     1441 ttacgatttc ctgtgccgga tggcggcgga ggaaggcatc ttcttttatg aggagcacgc
     1501 gcaaaaaagt accgaccaga gcctggtcct gtgcgatacc gtgcgttatc tgccggagtc
     1561 ctttgagatc ccctggaatc cgaacacccg taccgaggta agcaccctct gcatcagcca
     1621 gtttcgctac agcgcacaaa tccgcccttc ttccgtggtg accaaagact acacctttaa
     1681 acgacccggc tgggcagggc gttttgatca ggaaggccag taccaggatt accagcgcac
     1741 acagtatgaa gtgtatgact accccggacg tttcaagggt gcccacgggc agaactttgc
     1801 ccgctggcag atggatggct ggcgaaacaa cgcagaagtg gcgcgcggaa caagccgttc
     1861 gccggagata tggccgggac ggcgaattgt gctgacgggg catccgcagg cgaacctgaa
     1921 ccgggaatgg caggtggtgg cgagcgatct tcacggcgaa cagccgcagg cggtaccggg
     1981 acgcaggggt tcaggcacca cgctggataa ccattttgcg gtaataccgg cagacagaac
     2041 atggcgacca cagccgttgc tgaaaccgct ggtggatggc ccgcagagcg ccgtcgtgac
     2101 gggaccggca ggcgaggaaa tcttctgcga cgaacatggt cgcgtgcggg tgaaatttaa
     2161 ctgggaccgc tataacccgt caaaccagga cagttcatgc tggatccgtg tggcacaggc
     2221 gtgggcaggc accgggtttg gtaacctggc gataccgcgt gtgggtcagg aggtgattgt
     2281 ggacttcctc aacggcgatc cggaccagcc gatcattatg gggcgcacct accaccagga
     2341 aaaccgcaca cccggcagcc tgccgggaac aaagacgcag atgaccattc gttcgaaaac
     2401 ctataagggc agcgggttta atgaactgaa gtttgacgat gcgacaggga aagaacaggt
     2461 ctacatccac gcgcagaaga acatgaacac cgaggtgctg aataaccgca ccactgatgt
     2521 gataaacaac catgccgaaa aaataggtaa caaccaggcg atcaccgtta ccaataacca
     2581 gatccagaac attggcgtta atcagataca gacggttggt gtcaaccagg tggaaacggt
     2641 gggcagtaac cagattatca aagtgggatc aaaccaggtt gaaaaggtgg ggatcattcg
     2701 tgcgctgacg gtgggtgtgg cgtatcagac gacggtaggc ggcattatga atacctcggt
     2761 ggcgttgttg cagtcctcac aggtagggct gcataaatca ctgatggtgg gaatgggcta
     2821 cagcgtcaat gtagggaata acgtcacctt ctcggtgggc aagacgatga aggaaaacac
     2881 cggacaaaca gcagtttatt ctgccggtga gcatcttgaa ctctgctgtg gtaaggcaag
     2941 gctggtgctg acgaaggacg gaagcatatt tcttaatggt acgcacattc atctggaagg
     3001 ggagtcggat gtgaacggtg atgcgccggt gattaactgg aactgtggtg ccacacaacc
     3061 tgtaccggat gcgcctgtgc cgaaagattt accccccgga atgccggata tgcggcaatt
     3121 ttgagtcagg cgcaaaaaat caaaacggaa ctggtgcagt gaccagataa accatattaa
     3181 aagcagaagg aattgattca tgggcggaaa accggcggcg cggcagggtg atatgacccg
     3241 caagggactg gatattgtgc agggttcagc gggggtgctg ataggtgcgc cgacgggagt
     3301 ggcctgctcg gtgtgtccgg gagggattac ctatgctaac ccggtgaacc cgctactggg
     3361 tgcgaaggtg ctgccgggcg agacggacct tgcgctgccc ggcccgctgc cgttcattct
     3421 ttcccgtgcc tacagcagtt accggaccag gacgcccgcg ccggtggggg tgtttggccc
     3481 cggctggaaa gccccctttg atatccgctt acagattcat gagcgggaac tgatactcaa
     3541 cgacaacggc ggcaggagca ttcactttga gccgctgttt cccggcgaga taagctacag
     3601 ccgcagcgag tcactctggc tggcaagggg tggggtgctg aaacaacaca aaggccaccc
     3661 gctggcccgg ctctggcggg cgctgccgga agcggtccgt ctgagtccac acacctatat
     3721 gatggctgtc agcacaaccg ggcagtggct ggtgctcagc tggccggagc gggtgccgga
     3781 ggcggacgag gtgctgccgc cggagccgcc cgcataccgg gtgctgacgg gcgtggtgga
     3841 tggctttggt cggacgctgg cctttcacag ggcggcggag ggtgacgtgg cgggcgcggt
     3901 gacgggggtg acggacggcg cgggccgccg ttttcacctg gcgctgacca cacaggcgca
     3961 gcgggcggaa gccttccgta aacagcgcga gtcttcctta tcttccccgg ccagtccccg
     4021 ttctgtttcc tcttcttcct tttcttcagc tttccccgac acgctgcccg ccggtacaga
     4081 gtacggtgcc gataacggta tccggctgga ggcggtgtgg ctgacacacg acccggcata
     4141 cccggatgaa cagccgactg cgccgctggc gcgctacacg tacacggcca gcggagaact
     4201 gcgggcggtg tatgaccgca gcgggacgca ggtgcgcggg tttacttatg atgtgcagca
     4261 cgccgggcgg atggtggcgc accattatgc agggaggccg gagagccgct accggtatga
     4321 tgataccggt cgggtgacag aacaggtcaa cccggagggg ctggactacc gctttgagta
     4381 cgggcaggac cgtgtgacca tcacggacag cctgaaccgg cgggaggtgc tgtacacgga
     4441 gggcgaaggt ggcctgaagc gtgtggtgaa gaaggaacat gcggacggga gcatcacccg
     4501 cagcgagtat gatgaggcgg ggaggctgaa ggcgcagacg gatgcggcgg ggcggcggac
     4561 ggagtaccgg ctgcatatgg cgtctggaaa gctgacttca gtgatactgc cggacggcag
     4621 gacggtacgg tatggttaca acagccagtt gcaactgact tcagtgacat atccggatgg
     4681 tttacgcagc aaccggaaat atgaccgtca gggccgactg gcagaagaga cctcgcgcaa
     4741 cggtaacatt acgcgctggt tttatgactc cccccgcagt agcctgcccg gcgcggtgga
     4801 agacggaacc ggggtgacgc ggagaataac gcgaaaccgg tacggtcagc ttcgggcctt
     4861 cacggactgc tcggggtaca cgacgcggta tgagtatgac cggtacggtc aacaaatcgc
     4921 cgttcaccgg gaggaaggca tcagcactta cagcagttat aacccgcgtg ggcaactggt
     4981 cagtcagaag gatgcgcagg ggcgtgaaat ccgttatgag tacagcgccg caggcgacct
     5041 gacagcaacg atatcaccgg acggtaaacg cagcaccatc gaatatgata aacacgggcg
     5101 tccggtgagt gtgacggaag gcggtcttac gcgcagcatg gggtatgacg ctgccgggcg
     5161 catcaccgtg ctgaccaacg agaacggcag ccagtccacg ttccggtatg acccggtgga
     5221 caggctgact gaacagcgcg gttttgacgg ccggacgcaa cgttaccact atgacctgac
     5281 cggaaaactc acgcagagtg aagacgaggg gcttatcacc ctctggcact acgatgcgtc
     5341 ggaccgcatc acgcaccgga cggtgaacgg cgacccggca gagcagtggc agtatgatga
     5401 gcacgggtgg ttaaccaccc tcagccatac cagtgaaggc caccgcgtgt cggtccacta
     5461 cggctatgac gataaaggcc gcctgacggg cgaacggcag acggtggaga acccggagac
     5521 gggggagatg ctgtgggagc atgagacggg gcatgcgtac agcgaacagg ggctggcgac
     5581 ccgtcaggag ccggacggtc tgccgccggt ggagtggctg acgtatggca gcggttatct
     5641 tgcggggatg aagctgggcg gaacgccgct ggtcgagtac acgcgggacc ggctgcaccg
     5701 tgagacggcc cgcagcttcg gcggggaggc atatgaactt gccaccgcct ggaataccag
     5761 cggccagctc cggagcaggc acctgaacct tccgcagctt gaccgtgagt atacgtggaa
     5821 cgacaacgga cagctaatcc gcatcagcgg cccgcaggag agccgggagt accgttacag
     5881 cggcacggga aggctgacgg gcgtccacac cacggcagcg aacctggaca ttgatatccc
     5941 gtatgcgacg gacccggcag gaaaccggct gccggacccg gaactgcacc cggacagcac
     6001 gctcacggcg tggccggata accgcatcgc ggaggatgcg cactatgtct accgccacga
     6061 tgaatacggc aggctggcgg agaagacgga ccgtatcccg gaaggggtta tccggatgca
     6121 cgacgagcgc acccaccact atcactacga cagccagcac cgcctggtgt tctacacgcg
     6181 gatacagcac ggcgaaccac aggtggagag ccggtacctc tatgacccgc tgggccgccg
     6241 gacgggaaaa cgggtatggc ggcgggagcg tgacctgacg gggtggatgt cgctgtcgcg
     6301 taaaccggag gtgacatggt acgggtggga cggtgacagg ctgacgacga tacagaccgg
     6361 caccacgcgt atccagacgg tataccagcc ggggagcttc acgccgctcc tgagaatcga
     6421 aacagagaat ggcgaacagg cgaaggcgcg gcaccggagc ctggcggagg tgttgcagga
     6481 ggacacgggt gtgacgctac cggcggagct gtcggtgatg ctggggaggc tggagcggga
     6541 actgcgcgcg ggagcggtga gtgcggagag tgaggcatgg cttgcgcagt gcgggctgac
     6601 ggcggagcag atggccgcac agctggaggc ggaatacatc ccggagagga aacttcatct
     6661 ttaccactgc gaccaccggg gactgccgct ggcgctcatc agcccggaag gggaaacggc
     6721 gtggcagggg gagtatgacg agtggggaaa cctgctgggc gaaaccagcg cgcagcacct
     6781 tcaacagtca ctccgtctgc cggggcagca gtatgatgag gagtcggggc tgtactataa
     6841 ccggaaccgg tactacgatc cgttgcaggg acgctatatc acccaggatc ccattgggct
     6901 gaagggggga tggaacctgt atgcgtatcc gctgaacccg gtgcagcggg gggatcctct
     6961 tgggttgtct ggtgagacac tggttcttgg ttctgccaca acagtaacag gaacagaatt
     7021 tgggggggcg ttactttcgg gtggggcggc gacttttgat gcagtcacgg cggctatggc
     7081 gtctgctgcc cctgctcttg gagcaatggc tgtggtcgct gggccaatgg gatatgacct
     7141 tctcgctaat gatgggcgtg ccgggaaggc tctgagcgat gggcagttaa taaattctca
     7201 ggctgaaatg tgttactgga ccagtagttg tcctttagat agcccacatg agaatgataa
     7261 ttcgtcaggg agtcagccga atgttgggaa ggatttgacg gatgaagaga agaactgcct
     7321 ggccggcggc agtacgggaa gcccgggagg atggggacca gaagatgaga atagcgcaag
     7381 ggatcgtatg cagcatgaac ggtataaaga tgaattgcga cagaaaatgg gaaaacccga
     7441 cgttacagat tcaaatttga aaaatattat agacgatctt tatcggccaa atgcaaaagt
     7501 aggaagtgga agcacagcag atgctgttcg ttatgaattg gccacaggtg aaaaagttgg
     7561 tggtagagga catgtattga aagcacagga ttatacaagg gctttacagg attggttgga
     7621 taagaatcca acagcatctc caggggatcg agcagcagca gaaaacgttc ttcaggattt
     7681 acagaatgct ttgggaggca agtaatgaac caatatattg ctagttatat agaaaaaatg
     7741 gcatcatttt ccactgtttt gtctgagaca gtctcattag ctaatgaata ctggcatcca
     7801 gaaacgccac cg
//
LOCUS       DAPGOF010000209         7778 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.210, whole genome shotgun sequence.
ACCESSION   DAPGOF010000209 DAPGOF010000000
VERSION     DAPGOF010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.210"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(244..1437)
                     /gene="tyrB"
                     /locus_tag="RHV13_004190"
     CDS             complement(244..1437)
                     /gene="tyrB"
                     /locus_tag="RHV13_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HDV1243654.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVADAEARLNAQPHGASLYLPMEGLNSYRHAIVPLLFGADHPVLKQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(1690..2769)
                     /gene="alr"
                     /locus_tag="RHV13_004191"
     CDS             complement(1690..2769)
                     /gene="alr"
                     /locus_tag="RHV13_004191"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDV1243655.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(2822..4237)
                     /gene="dnaB"
                     /locus_tag="RHV13_004192"
     CDS             complement(2822..4237)
                     /gene="dnaB"
                     /locus_tag="RHV13_004192"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDV1243656.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4320..5303
                     /locus_tag="RHV13_004193"
     CDS             4320..5303
                     /locus_tag="RHV13_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HDV1243657.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNII
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(5469..5711)
                     /gene="pspG"
                     /locus_tag="RHV13_004194"
     CDS             complement(5469..5711)
                     /gene="pspG"
                     /locus_tag="RHV13_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HDV1243658.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(5845..6882)
                     /gene="dusA"
                     /locus_tag="RHV13_004195"
     CDS             complement(5845..6882)
                     /gene="dusA"
                     /locus_tag="RHV13_004195"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HDV1243659.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(7245..>7778)
                     /locus_tag="RHV13_004196"
     CDS             complement(7245..>7778)
                     /locus_tag="RHV13_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HDV1243660.1"
                     /translation="GLPSTVQLFYEESKDNEPLANIHLQAIGSGFLTFVNACQEYDDN
                     SLKLFASLLISLSYSSAYTDLAGAEKVNINDDNENYLTAQFEELSQRDMKKYLGEMKR
                     LADGGEMNFDGYLDKMSHLVNEGKLAPDILSKMRDAAPKLIDFAKSFDPNSKEKIKIY
                     TDTSKLIYDLFGVKSEK"
BASE COUNT         2009 a         2033 c         1851 g         1885 t
ORIGIN      
        1 caaacccgat acttattatt aaatttaata atggttagat gtgcagaaat tattactcca
       61 cccaaaaaaa caggataatg aattccagat aattgggata agcttaacaa taaaagaact
      121 aacaacgttt attatctctc acgtagaacg atggcatcaa aaaaatgatc tataaagaaa
      181 gagtgaaagc gacgacagtt taatttcact gcaggctggg tagctccagc ctgctttcct
      241 gcattacatc accgcagcaa acgcctttgc cacacgttgt acatttgccg tatttaaccc
      301 agcgacacac atgcgaccac tggcgatgag atagacacca aattcttcac gtagtcggtc
      361 aacctgagcg gcacttaaac cggtataact gaacatgccg cgctgattaa gcagataatc
      421 gaaattgcgt tctggcatct ctgtgcttaa taccttcacc agttcctgac gcattgccag
      481 aatgcgagta cgcatctctt ctacttccgc cagccagcta gctttcaatg cctcgtcatt
      541 cagcactgca gccaccacct gcgcaccaaa attcggcggg ctggagtagt tgcggcgaac
      601 tgttgctttc aattgcccca gtacgcggcc tgcggcttcg gcatcttcac acagaacaga
      661 aagtccgcca acgcgctcgc cgtaaaggga gaaaattttc gagaacgaat tgctcaccag
      721 agcgggtaat ccagcgctgg caatggcgcg aatggcgtag gcatcctctt ccataccggc
      781 accaaatcct tgataggcaa tatcgaggaa tggaataagc tcgcgggctt tgagaatttc
      841 aatcaccgca tcccactggt cattagtgag atcggcaccc gttgggttgt ggcaacatgg
      901 atgcagcaac acaatactgc gggcaggtaa tgtttttagc gtcgccaaca ggtcattaaa
      961 gcgcacgccg ttagtcgctt cgtcatacca ggggtaagta cttacttcga atccagcccc
     1021 ggcgaagatt gctacgtggt tttcccaggt aggatcgctg acccagacgc ctgattccgg
     1081 gaagtagcgt ttcaggaaat ccgcgcccac tttcaatgcc cctgagccgc caagggtttg
     1141 aatggttgct acgcgctgtt gtttcagtac cggatggtcc gcaccaaaca gcagcggcac
     1201 aatggcatgg cgatagctgt taagcccttc catcggtaaa taaagcgaag cgccatgagg
     1261 ctgcgcattc aggcgcgctt ctgcatcagc cacggctttc agttgcggaa taattccgtc
     1321 ttcgttgtag tacagaccga tacttaaatt cactttgtcg ctgcgagggt cttctttaaa
     1381 acgctccata agcgtaagaa tcgggtcgcc agcgtaggcg tcaacttttt gaaacacgcg
     1441 atggttctcc aggtttacgg gcaggtggtt aaaacacaat aaaccggaag aaggcgaaga
     1501 tcgagtggat gttcaggggc gaacggcaat tagcaacaga gtgagactca tgacaaacgt
     1561 acatccgcca gaggcacgac ctttataaga gcattaaaga atatcaactt attgaattag
     1621 taggatttta ttggccggat aaggcattca cgccgcatcc ggcacagaca atcaaatatt
     1681 acagaacgat taatccacgt atttcatcgc gacccttgaa gtcaggcgcg tgataagttc
     1741 gtaagcgctc acttttgtca tttcagcgat acgttctacg ggcaaacctt cgccccataa
     1801 aatgaccggg tccccggctt tatcctgtgc ctgtggacct aagtctacgc agatcatatc
     1861 catcgccacg cgccccacaa tcggcacttc gcgaccattc accagcactg gcgtaccgga
     1921 cggcgcggcg cgcggataac catcgccata gcccatcgcg actacgccaa gacgggtatc
     1981 acgttcgctt acccaggttc caccataacc aacaggctct ccggctttat gctcacgcac
     2041 ggcaatcagg ctggaggtta gtgacatcac tggctgacag ccaaaatcgg caccggtgga
     2101 gcgatcttcc agcggcgaga cgccataaag aatgatgccc gggcgcaccc agtcaaaatg
     2161 cgactgtggc cacagcagaa tgccacccga cgcggcaatg gaacgttgac ctggtttgcc
     2221 ttcgcaaaag gtattaaaga tagcgagttg tttctcggtt gcgccacatt ttggttcatc
     2281 cgcgcgcgca aaatggctga cgatattcac cggctgacga acgtttttgc actgggtcag
     2341 gcgatgataa aacgcctcag cctgttccgg ccttacgccc agacggtgca taccggtatc
     2401 gagtttcatc cagacggtaa ccggctcgtc caggctagcc tcttccagcg cagccagctg
     2461 ttcttcgtta tgcacggcgg tatgaaaatg ttgcgcagaa atcgtcggca gatctctggc
     2521 atcaaaaaag ccttcgagta acagtacagg tttggtgatt ccccccgcac gcagtcgcag
     2581 agcttcttcg agacgggcta cgccaaaggc gtcagcatcg gggagcgttc gcgcggtctc
     2641 aagaagaccg tgaccataag cgttcgcttt caccaccgca accattttac tggcaggggc
     2701 cagttcacga agacgttgca ggttgtgtcg cagagcgcgg cggttaatca caacagttgc
     2761 cgcttgcatt tgtgttcctt gataagtgtt tgctttaatt acctaattca taaaataatt
     2821 attattcgtc gtcgtactgc ggccccgcat agttgtcgaa gcgcgaccat tgaccgttaa
     2881 aggtcaggcg aaccgtcccg attgggccgt tacgttgttt accgataata atttccgcga
     2941 tgccttttaa atcactgttt tcgtgataca cctcatcacg atagataaac atgatcaagt
     3001 ccgcatcctg ctcgatagag ccagattcac gcaggtcgga gttgaccgga cgtttgtcgg
     3061 cacgttgttc cagagaacgg ttcaactggg acagcgccac cactggcacg ttcagttctt
     3121 tcgccagtgc tttcagcgag cgggagattt ctgcaatttc cagcgtacgg ttatcggaaa
     3181 gcgccggtac gcgcatcagt tgcaggtagt cgatcatgat aagcccgatg ccgccgtgtt
     3241 cacgggcaat acggcgtgcg cgggaacgca cttccgttgg cgtcaggccg gaggagtcat
     3301 cgatatagat attgcgtttt tcgagcaaaa tacccatggt gccggaaatg cgcgcccagt
     3361 cttcatcatc gagctgcccg gtacggattt tagtctggtc aacgcgcgac agcgacgcca
     3421 gagaacgcat catgatctgt tctgatggca tctccagtga gaagataagc accggcttat
     3481 cctgcaacat cgccgcgttc tcgacgaggt tcatcgcaaa tgtcgtttta cccatcgacg
     3541 gacgcgcggc gacgatgatc aaatccgacg gctgcaagcc agcggttttt ttattgagat
     3601 cgtcataacc ggtgtttacc ccagtaacgc catcgtgtgg ctgctgaaac aactgctcaa
     3661 tacgcgccac ggttgcgtcg agcacatcgg cgatgttctt cggcccttcg tctttattag
     3721 cccggctctc ggcaatttta aagacacggg attcagccag atccagcaga tcttcgctgg
     3781 tacgcccctg cggatcaaaa ccagcttcgg caatctcatt cgcaaccgag atcatctcac
     3841 ggacaacggc acgttcacgc acgatgtccg cataggcact gatgttcgca gcacttggcg
     3901 tattttttga cagttctgcc agataagcaa atccaccgac gctatcgagt tgcccctggc
     3961 gttccagcga ttccgcaagg gtaatcagat cgataggact accgctttcc tgcaaacgcg
     4021 ccatttcagt aaagatatga cggtgtgggc gggtgtaaaa atcgtctgct accacacgct
     4081 cggctacatc atcccagcgt tcgttatcca gcattaaacc gcccaacacc gactgctccg
     4141 cttcgatcga gtgcggaggc actttcagcc cggcaacttg tggatcgcgt tcgcggggtt
     4201 cagcctgctg tttgttgaag ggtttatttc ctgccatagt gaatggagtt accgagataa
     4261 agaatgggtc gaaactttac catatgaagc agaccctgac gatacgttct ggaggacaca
     4321 tggcaacacg aattgaattt cacaagcacg gtggcccgga agtacttcaa gccgtagagt
     4381 tcactcctgc cgatccggcg gagaatgaaa tccaggtcga aaataaagcc atcggcatca
     4441 attttatcga cacatatatc cgcagcggcc tttacccgcc gccatcgcta cccagcggat
     4501 taggcaccga agcggcaggc atcgtgagta aagtcggcag tggtgtaaaa catattaagg
     4561 caggcgatcg tgtagtctat gcgcagtcgg cgttaggcgc ttacagctct gtgcataaca
     4621 ttattgcgga taaagcggcg attctgcctg cggcaatttc ttttgagcaa gctgcggcat
     4681 ccttcctgaa aggcttaacg gtttattatc tgctacgcaa aacctatgaa attaaacccg
     4741 atgagcagtt cctgttccac gcagcagctg gcggcgttgg cttgattgcc tgccagtggg
     4801 caaaagccct gggcgcgaaa cttatcggca ccgtaggaac cgcgcaaaaa gcgcagagcg
     4861 cgctaaaagc cggcgcgtgg caggttatta actatcgcga agagaatctg gtcgagcgat
     4921 tgaaagagat caccggcggt aagaaagtgc gcgtagtgta cgactccgtg ggcagagaca
     4981 cctgggaacg gtcgctggat tgcctgcaac gccgcggctt aatggtgagt tttggcaact
     5041 catcaggtgc ggttaccggt gtgaacttag gcattctcaa tcagaaaggc tcgttgtatg
     5101 tgacacgccc ttccctgcaa ggctatatca ccacgcggga ggaattaacc gaggccagta
     5161 atgaactgtt ctctttgatt gccagcggtg tgattaaggt cgatgtcgcc gagcagcaga
     5221 aatatccgct gaaggatgcg cagcgtgcgc atgagattct ggaaagccgg gcgacgcaag
     5281 gttccagcct gttgattcca taaaagaaat agggcttcca cctgggaagc cctttctttt
     5341 tatagttcgg ctgtatgtag ggtacagcac gatgaatctg ttagaggcgc aatagtgaca
     5401 gatttgatta tcaattccta ttttgttcta aggataaaac cttaggttgt gatcatccgc
     5461 acaatccctt agtaacgcca gcggtcataa cgctgatatt tcggcacttt tggtgcttta
     5521 atcgccttaa taacccacac caccgcaatc gccaggagta accacggcag caacttaatc
     5581 atcaatgcca gcataccgcc gaggaacata atggccgtcg ccacaaccag cgcggcgata
     5641 atgcccagca acgaaacgcc ggtgaccatc aacatgacaa aaaagccaat cacaaaaagt
     5701 agttccagca tgatgctctc ccaaatatga aatctcttgc tggcattaca agaatcatgc
     5761 caaaaataat ctattgattt aacagcaaaa cgccccgcga cggtgcgcag ggcgtggtga
     5821 atttgactat tttttggtga aaagttaacg tttatccgcc accagtttga gcgcgtgttc
     5881 cagcacatta atgtctgcac ccgctttatg ggcattttca cttaaataac gccgccactg
     5941 ccgcgcgcca ggaataccct ggaacaggcc cagcatatgc cgggtaatat ggccgagata
     6001 cgtcccctgg ctgagttcac gctcaatgta cggatacatg gcgcgcacta ccgccaccgg
     6061 atcggcatcg gtatccgagg aaccaaagat ctctcggtct accgccgcca gaatacccgg
     6121 attctgatac gcctcgcgcc cgaccatcac gccatccata tgttgcaaat gagcttttgc
     6181 ctcttccagc gacttgatac caccgttaat cgacattgtc agatgcggaa agtcacgctt
     6241 cagttgatac acacgcggat aatcgagcgg cggaatttca cggttttctt tcgggcttaa
     6301 cccagaaagc caggctttac gtgcgtggat gatgaacatt tcacactcgc ctttgccgga
     6361 aacggtgttg atgaaatcgc agagaaattc atagctgtcc tggtcatcga tgccaatacg
     6421 cgttttcacc gtcaccggaa tcgacaccac atcgcgcatc gctttcacgc agtcggcaac
     6481 cagctgcgca ttacccatca gacacgcacc aaacatgccg ttctgcaccc ggtcagacgg
     6541 gcagccgaca ttcaggttga tctcatcata tccacgcgct tctgccagct tcgcacactg
     6601 cgccagcgcc gccggatcgc taccgccgag ttgcaacgct accggatgtt cttcttcact
     6661 gtacgccaga taatcacctt taccgtgaat aatcgcccct gtggtcacca tttcggtata
     6721 cagcaacgta ttgcgggaaa gcagacgcaa gaaatagcgg caatgtctgt ccgtccagtc
     6781 gagcattggt gcaacgctaa accgaccact ccagtgaacg tcagtttttt caggcattgc
     6841 gctggtttgg ttgatttttt gcatttcaga attaccgtgc attttcaaat gtggagatta
     6901 ttttatcgat atatcattga gttatgttat tcagcatcac tgttcaggag gctcaatagc
     6961 gggatactat accataacaa caggaagcgc ctgtctcatt gcaaaagaaa attgagatcc
     7021 tctcaaggca tgaagctctc acgaagtgat ggaaataatc ttattagccg ttagctttgt
     7081 taaggccaat gataaacaat ccaggttcga cgataaataa aaatcacaca ttaaactccg
     7141 gtgatatctc tccctgctaa tagcactgat agagaaaaaa agaacccaat aagcattggg
     7201 ttcttttatg taatgccttc cataccatcg aagaacttca catattattt ctccgattta
     7261 accccgaata gatcataaat taatttagaa gtgtctgtat atattttaat cttttccttt
     7321 gagtttgggt caaacgactt agcaaagtca attaatttcg gtgcagcatc tcgcattttg
     7381 cttaaaatat caggggcgag ctttccttca ttcaccagat gtgacatctt atccagatag
     7441 ccatcaaaat tcatttctcc cccatctgcc agacgcttca tctctcccag gtacttcttc
     7501 atatcacgtt gagataactc ttcaaactga gctgtcagat agttctcatt atcatcatta
     7561 atattcactt tttctgctcc tgctaaatct gtgtaggcac tagagtatga aagtgaaatc
     7621 aacagcgaag cgaataattt taagctgttg tcatcatatt cctgacatgc attaacaaac
     7681 gttaaaaaac cagaacctat tgcttgtaaa tgtatatttg ctaaaggttc attatcttta
     7741 gattcttcat aaaataactg aaccgtggag ggaaggcc
//
LOCUS       DAPGOF010000210         7589 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.211, whole genome shotgun sequence.
ACCESSION   DAPGOF010000210 DAPGOF010000000
VERSION     DAPGOF010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.211"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            164..271
                     /locus_tag="RHV13_004197"
     CDS             164..271
                     /locus_tag="RHV13_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243661.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(324..1082)
                     /locus_tag="RHV13_004198"
     CDS             complement(324..1082)
                     /locus_tag="RHV13_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HDV1243662.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            1369..2298
                     /gene="pfkB"
                     /locus_tag="RHV13_004199"
     CDS             1369..2298
                     /gene="pfkB"
                     /locus_tag="RHV13_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HDV1243663.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            2399..2689
                     /gene="ghoS"
                     /locus_tag="RHV13_004200"
     CDS             2399..2689
                     /gene="ghoS"
                     /locus_tag="RHV13_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HDV1243664.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            2795..3655
                     /locus_tag="RHV13_004201"
     CDS             2795..3655
                     /locus_tag="RHV13_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HDV1243665.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGRDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNALSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(3696..4232)
                     /locus_tag="RHV13_004202"
     CDS             complement(3696..4232)
                     /locus_tag="RHV13_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HDV1243666.1"
                     /translation="MTYQQAGRIAILKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            4379..5047
                     /gene="hxpB"
                     /locus_tag="RHV13_004203"
     CDS             4379..5047
                     /gene="hxpB"
                     /locus_tag="RHV13_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HDV1243667.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTDLTAK
                     DLLG"
     gene            5210..5800
                     /locus_tag="RHV13_004204"
     CDS             5210..5800
                     /locus_tag="RHV13_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDV1243668.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            5933..7324
                     /gene="tcyP"
                     /locus_tag="RHV13_004205"
     CDS             5933..7324
                     /gene="tcyP"
                     /locus_tag="RHV13_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HDV1243669.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHH"
     gene            complement(7328..>7589)
                     /locus_tag="RHV13_004206"
     CDS             complement(7328..>7589)
                     /locus_tag="RHV13_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243670.1"
                     /translation="PLNLPGMGSGILEDIMSIPDSSLRKRLGYEVLSFSLQAHSLSQE
                     CIDKLDIFFADDLFKYESVCIAAMEHLKSKATAPIQNGPLPA"
BASE COUNT         1909 a         1847 c         1870 g         1963 t
ORIGIN      
        1 gatatttgta tatcgataca taattttatt gttttcataa aacgcagaaa cagtgaccat
       61 gtgcacaacg ccacccgacg gctgtaataa caaaaaatcc cttgatgcct gtcccttttg
      121 ttacactccg ttatcacgca caagagatat gcaggacact ggtatgccga ctaaacgctt
      181 tgataaaaaa cactggaaga tggtggtggt gctactggca atctgtggcg ctatgttgtt
      241 gctacgttgg gcagcaatga tttggggctg agcagtgaca aacgaagaca gccagaccgt
      301 ataacacggt ccggcagata attttacatg ctgtagccca gcgataaggt tgtccgacgg
      361 tcggtatgtt ccggtgcaga ctctggaggt tcagagttcc atgtgacgtt gtaagccacc
      421 ttcagtccaa aatgttcatt aatagcaaca tttaatgcgc tttcggagtt cagcgttgtg
      481 tcttccgcgc caaagacgga aacaccctgc gtaaatttag cgttgtcagt cagctgccat
      541 gcataagccc cggaggcata gcccagcggc tgggtttcac tggcattgtc ggtatatttg
      601 tcgtaacgca cacctggacc gaattcaaag cggaaactgt gtaccgggcc attcaaaaac
      661 tgacgaccat aacccgcggt caacacatcg cgctctcgat agccgttata acggtctgtc
      721 agccagcttg cctgaccaaa taaatagtca taatcagtta aattaaaacg gctacgcccg
      781 cccgccgcat atttttctga agaacgctca tcattagaag aagtattact ggcgttaccc
      841 cacagcgacc aggcagtggt ttgtccatac caggtcatgg tggtgtcagc cgtaagtgag
      901 gagcttttcg tattgcctga ttgtgcaaga tatcctgcgt tcagattacc ttcaaaaggt
      961 tttttagcgc tggcaggatc gtccatgaca gtaaaaacgg aatcatcggc agctgcattc
     1021 agtgacgcaa acatgccccc cgccaacata acgatggcag gaactgtctt caaaagcttc
     1081 atttatcaag agtccgtaca acaaaaaaag agaccatcgc ggtcccggaa actttcttaa
     1141 ggatcaaaga ttagcgtccc tggaaaggta acgaattata aaaaggctcg aataacttag
     1201 caatgtattc ttatttcatt ttttgaataa gcatgtggcg aaaacagatt tttatttata
     1261 tatatttatc tgcaaaattt taaataaagc tctaataaat catattgtta atttcttcac
     1321 tttccgctga ttcggtgcca gactgaaatc agcctatagg aggaaatgat ggtacgtatc
     1381 tatacgttga cacttgcgcc ctctctcgat agcgcaacaa ttaccccgca aatttatccc
     1441 gaaggaaaac tgcgctgtac cgcaccggtg ttcgaacccg ggggcggcgg catcaacgtc
     1501 gcccgcgcca ttgcccatct tggaggcagt gccacagcga tcttcccggc gggtggcgcg
     1561 accggcgaac acctggtttc actgttggcg gatgaaaatg tccccgtcgc tactgtagaa
     1621 gccaaagact ggacccggca gaatttacac gtacatgtgg aagcaagcgg tgagcagtat
     1681 cgttttgtta tgccaggcgc ggcattaaat gaagatgagt ttcgccagct tgaagagcaa
     1741 gttctggaaa ttgaatccgg ggccatcctg gtcataagcg gaagcctgcc gccaggtgtg
     1801 aagctggaaa aattaaccca actgatttcc gctgcgcaaa accaagggat ccgctgcatc
     1861 atcgacagtt ctggcgaagc gttaagtgca gcactggcga ttggtaacat cgagttggtt
     1921 aagcctaacc aaaaagaact cagtgcgctg gtgaatcgcg aactcaccca gccagacgat
     1981 gtccgcaaag ccgcgcagga aatcgttaat agcggcaagg ccaaacgggt tgtcgtttcc
     2041 ctgggtccac aaggagcgct gggtgttgat agtgaaaact gcattcaggt ggtgccacca
     2101 ccggtgaaaa gccagagtac cgttggcgct ggtgacagca tggtcggcgc gatgacactg
     2161 aaactggcag aaaatgcctc tcttgaagag atggttcgtt ttggcgtagc tgcggggagt
     2221 gcagccacac tcaatcaggg aacacgtctg tgctcccatg acgatacgca aaaaatttac
     2281 gcttaccttt cccgctaaca aaaacattcc cccagcattg ggggaatcat caccaacctg
     2341 tcggcaacgc gtttctccga ctatgctcaa aagtcatgtg ataacaaagg ggtgaactat
     2401 ggccagtggc gatcttgtcc gttatgtcat aaccgtcatg ttgcatgagg atacattgac
     2461 tgaaattaac gagttgaata attacctgac tcgcgacggt tttttgctca ccatgacgga
     2521 tgatgaggga aatatccatg agctggggac taacactttt ggacttatca gtacccaaag
     2581 tgaagaagaa attagagaac tggtttcggg gcttacccaa agtgcaaccg gcaaagatcc
     2641 tgaaatcacc atcacgacct gggaggaatg gaatagcaac agaaaataaa tggtttttgg
     2701 gcaataatca gtctgtggtg tgcgttagct cgtgttttta caccgcattc ttgcgctaac
     2761 cttatgatct ggcagacaac atgggagaga catcatgtgg caggcaatca gtcgtctttt
     2821 gagcgagcag ttaggtgaag gcgaaatcga actgcgtaat gaactgcctg gcggagaagt
     2881 ccatgccgca tggcatttgc gctatgcagg acgtgacttt ttcgtcaaat gtgatgaaag
     2941 ggaactgctt cccggtttta ccgccgaagc cgaccaactg gagttactgt cgcgtagtaa
     3001 aaccgtcacc gtgcctaagg tttgggcagt tggcgctgac cgtgactaca gttttctggt
     3061 gatggattat ctcccacctc gtccgctgga tgcgcatagc gcatttattc ttggtcagca
     3121 aattgcgcgt ttacatcaat ggagtgacca accacaattt ggcctcgatt tcgataacgc
     3181 gctctccaca actccacagc ccaacacctg gcaacgtcgc tggtcaacgt tttttgctga
     3241 acaacggatt ggctggcagt tggaactggc agcagagaaa gggatcgctt tcggcaatat
     3301 cgacgccatc gtagagcata ttcagcagcg tctcgcctca catcaaccgc agccttctct
     3361 gttgcacggc gatttatggt ccggcaactg tgcgctgggt ccggatggcc cgtacatttt
     3421 cgacccggcc tgctactggg gtgaccgaga gtgcgacctg gcgatgttac cgctgcatac
     3481 tgaacagccg ccacaaatct atgacggcta tcagtcagta tccccgctac ctgccgattt
     3541 ccttgaacgt caaccggttt accaactcta cacgctgtta aatcgtgcaa ggttatttgg
     3601 cggtcagcat ttggttattg ctcagcagtc attggataga ttattagcag catgatatgg
     3661 gttgaggata atggccgctc cgtgcggcct tttgattaaa taaacccaag cagagaaaag
     3721 aacacataac ctgcagcaat acaaataacc ggcaggatat acagtgagaa aaactgcagg
     3781 aaaatagtat ggtgcggaac aacaatacgg gactcaattt gttcacgcgt tagcccctct
     3841 tcccctttgg ctttttccag aatgagttga tcttcaacac cttcccgcaa gaagcgcgcc
     3901 tggcgactca tccgggcacc ggaatcctgc aacgccagtc cgacaaaaat caggatgaaa
     3961 atcacccaga acataacgtt cacgccacca ttaaaattgg gcgtcgggga gttataccag
     4021 aacagattca aaaaaggcgt attcgcctgc atcatatcga tcatgacatg agtaaagtcg
     4081 agcatgaccg cattaatgcc ttcctggttt tcctgtcggg tattcataaa tttcagcaaa
     4141 gaaatcaatg tagagatcaa tgcaggtata aaaatcaccc aacccaaaat ccttttcaaa
     4201 atagcaatgc gtccagcttg ttgatacgtc atgagttctc cttgattaag acgcgtcgtt
     4261 tcacttagtt tacctgtaga tatctgtttt cgcccattct ttaaaggcga tatgataggc
     4321 gcttaatcat aagcacggct taatacctta cacataatgc tctaaaggag aggttgtaat
     4381 gtcaaccccg cgtcagattc ttgctgcaat ttttgatatg gatggattac ttatcgactc
     4441 agaaccttta tgggatcgag ccgaactgga tgtgatggca agcctggggg tggatatctc
     4501 ccgtcgtaac gagctgccgg ataccttagg tttacgcatc gatatggtgg tcgatctttg
     4561 gtacgcccgg caaccgtgga atgggccaag ccgtcaggaa gtagtagaac gggttattgc
     4621 ccgtgccatt tcactggttg aagagacacg tccattatta ccaggcgtgc gcgaagctgt
     4681 tgcgttatgc aaagaacaag gtttattggt gggactggcc tccgcgtcac cactacatat
     4741 gctggaaaaa gtgttgacca tgtttgactt acgcgacagt ttcgatgccc tcgcctcggc
     4801 tgaaaaactg ccttacagca agccgcatcc gcaagtatat ctcgactgcg cagcaaaact
     4861 gggcgttgac ccactgacct gcgtggcgct ggaagactcg gtaaatggca tgatcgcttc
     4921 taaagcagcc cgcatgcgtt ccatcgtcgt tcctgcgcca gaagcgcaaa atgatccacg
     4981 ttttgtatta gcaaacgtca aactctcatc gctgacagat ctcaccgcaa aagaccttct
     5041 cggttaatga ccaggggcag tgatcacgct gcccctggtt ctttatctga atttgcattc
     5101 aactgacgga ttaatcgtca atttaagaga aagagttaca ccgtcaccac ttccgtgcac
     5161 tgtataaaaa tcctatactg tacgtatcga cagtttagtg agttttatca tgacggcgga
     5221 aggtcacctt ctcttttcta ttgcttgtgc ggtatttgcc aaaaatgccg agctgacgcc
     5281 cgtgctggca cagggtgact ggtggcatat tgtcccttct gcaatcctga cgtgtttgtt
     5341 accggacatc gatcacccaa agtcgtttct tgggcagcga ttaaagtgga tatcaaaacc
     5401 gatcgcccgc gcttttgggc atcgtggttt tacccacagt ctgctggcgg tatttgcgct
     5461 gctggcaacc ttttacctta aggttcctga aagctggttc attccggctg atgcgctaca
     5521 aggaatggtg ctgggttatt taagccacat acttgccgat atgctgacac ccgccggtgt
     5581 tcccctgctc tggccatgcc gctggcgttt ccgcttgcct atcctggttc cccaaaaggg
     5641 taaccaactg gaacgtttta tctgcatggc attatttgtc tggtcggtat ggatgcctca
     5701 ttcattaccc gaaaacagcg ctgttcgttg gtcatcgcaa atgatcaata ccttgcagat
     5761 ccagtttcat cggcttatta agcatcaggt tgaatactaa aaaggcaaaa atcgcctttc
     5821 tgtaataagc aattccattt gaatataaga gccagctcac agttctgtta atcttgcgcc
     5881 aacactatga ctgctacgca gtgatagaaa taataagatc aggagaacgg ggatgaactt
     5941 tccattaatt gcgaacatcg tggtgttcgt tgtactgctg tttgcgctgg ctcagacccg
     6001 ccataaacag tggagtctgg cgaaaaaagt gctggtgggt ctggtgatgg gtgtggtttt
     6061 tggccttgcc ctgcatacca tttatggttc tgacagccag gtacttaaag attctgtaca
     6121 gtggtttaac atcgttggta acggctatgt tcaactgctg caaatgatcg ttatgccgtt
     6181 agtcttcgcc tctattctga gcgcggttgc ccgtctgcat aacgcatctc agttaggtaa
     6241 aatcagtttt ctgaccatcg gtacgctttt gtttaccacg ctgattgcgg cgctggtcgg
     6301 tgtgctggtc accaacctgt ttggtttgac ggctgaaggt ctggttcagg gtggtgcaga
     6361 aactgcacgt ctgaacgcca tcgaaagtaa ctatgttggt aaagtctctg acctgagcgt
     6421 tccgcagctg gtcttgtcct ttatcccgaa aaacccgttt gccgatctga ccggagccaa
     6481 tccgacgtca attatcagcg tggtcatttt tgccgcattc ctcggtgtag ctgcgctgaa
     6541 actgctgaag gatgatgcgc cgaaaggtga acgcgtctta accgctatcg ataccctgca
     6601 aagctgggtg atgaaactgg ttcgcctggt catgcagttg accccttacg gcgttctggc
     6661 actaatgacc aaagtggttg caggttctaa cctgcaagac atcatcaaac tgggaagttt
     6721 cgttgtcgcg tcctacctcg gtctgctgat tatgtttgca gtgcatggca ttctgctggg
     6781 cattaatggc gtgagtccgc tgaagtactt ccgtaaggta tggcctgtgc tgacgtttgc
     6841 ctttaccagc cgttccagtg ctgcgtctat cccactgaat gtggaagcac aaacgcgtcg
     6901 tctgggcgtt cctgaatcca tcgccagttt cgccgcctct ttcggtgcga ccattggtca
     6961 gaacggctgc gccggtttgt atccggcaat gctggcggtg atggttgcgc ctacggttgg
     7021 cattaacccg ctggacccga tgtggattgc gacgctggtc ggtattgtta ccgttagctc
     7081 cgcaggcgtt gccggtgtcg gtggtggtgc aactttcgcc gcactgattg tgctgcctgc
     7141 gatgggcctg ccagtaaccc tggtggcgct gttaatctcc gttgaaccgc ttatcgacat
     7201 gggccgtacg gcgctaaacg ttagtggctc gatgacagct ggcacgctga ccagccagtg
     7261 gctgaagcaa accgataaag ccattctgga tagcgaagac gacgccgaac tggcacacca
     7321 ttaattctta tgctggcaac ggtccgtttt gtataggggc cgttgcctta ctttttaaat
     7381 gttccatcgc ggcgatgcat actgactcat atttaaacag atcgtcagcg aaaaaaatgt
     7441 ccaacttatc aatacattcc tggctcaatg aatgtgcctg tagcgagaag cttaaaacct
     7501 catacccgag acgttttcta agtgaactat ctggaatact cataatatct tcaagtatac
     7561 cacttcccat tcccggaaga tttagtgga
//
LOCUS       DAPGOF010000211         7347 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.212, whole genome shotgun sequence.
ACCESSION   DAPGOF010000211 DAPGOF010000000
VERSION     DAPGOF010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.212"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..599)
                     /locus_tag="RHV13_004207"
     CDS             complement(<1..599)
                     /locus_tag="RHV13_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HDV1243671.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADK"
     gene            complement(612..2393)
                     /gene="gcl"
                     /locus_tag="RHV13_004208"
     CDS             complement(612..2393)
                     /gene="gcl"
                     /locus_tag="RHV13_004208"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HDV1243672.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRTQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(2483..3298)
                     /gene="allR"
                     /locus_tag="RHV13_004209"
     CDS             complement(2483..3298)
                     /gene="allR"
                     /locus_tag="RHV13_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HDV1243673.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(3376..3858)
                     /gene="allA"
                     /locus_tag="RHV13_004210"
     CDS             complement(3376..3858)
                     /gene="allA"
                     /locus_tag="RHV13_004210"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HDV1243674.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQHVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            4088..5014
                     /gene="allS"
                     /locus_tag="RHV13_004211"
     CDS             4088..5014
                     /gene="allS"
                     /locus_tag="RHV13_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HDV1243675.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            5083..6177
                     /gene="mnmH"
                     /locus_tag="RHV13_004212"
     CDS             5083..6177
                     /gene="mnmH"
                     /locus_tag="RHV13_004212"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HDV1243676.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLHAAGIDYPLVAGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAGRLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAQ"
     gene            6293..6658
                     /locus_tag="RHV13_004213"
                     /pseudo
     CDS             6293..6658
                     /locus_tag="RHV13_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(7179..>7347)
                     /locus_tag="RHV13_004214"
     CDS             complement(7179..>7347)
                     /locus_tag="RHV13_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243677.1"
                     /translation="KIVSENDPDTKKVWCKYGKIPGQGDSVNLFFVGEINVTHYFITN
                     IGAGLPDACAE"
BASE COUNT         1882 a         1907 c         1760 g         1798 t
ORIGIN      
        1 attttatcgg cccactgagt catggtgttg gttaattcac cttccatccg ctgcatgtga
       61 taaatgtcat actgaatttt caaattgcag caaccaacat catcaatcaa tttcagcgcc
      121 tgccgagttc cggtgagatg gaaaccagga atatcaaaat ggttaatagg ttcaatcagt
      181 aataaaatat cttctttcat cagcatattc gcggcataac gcaggttttc tacaagcgtt
      241 gcgtgaatct gttcactgct gaaaccagcc ggcgttttac cgaccagaca gttaattttt
      301 ttattaccca gcgcacgggc ataacgaatc gctgctgcta cgccatcccg aaactcttct
      361 tcacggccag gaatacaggc aataccgcgc tcccccgccg cccagtcacc cgccggtaaa
      421 ttgtgcagcg tatgttcgag tttattactc gccagcacct gttttaattc ttcaatgtcg
      481 tagtcataag gaaacataaa ttcaacgccg cgaaaaccac actgcgcagc tttctcaaaa
      541 cgggcgagaa aatcatattc accaaataac atcgataaat tagcagagaa acgtaacatt
      601 atttatctcc cttattcata gtgcatgaag caggtttcag tcggtgcgtc cgctgcgtta
      661 tcggcgatat cttcaaattc catgacgtta tccagttcgc tgcccatcga aatattggtc
      721 acacgctcga gaataacttc cacgactacc ggtacccgat attgcgccat taaggctttc
      781 gcctgttcaa aggctggcgc aatatcttcc ggtttgaaga cgcgaatagc tttacaacct
      841 aaaccttccg ctacttttac gtggtcgacg ccgtaaccgt tcacttcgct ggagttgata
      901 ttctcgaaag cgagttgcac gcagtagtcc atgtcaaagg cgcgttgtga ctggcgaatc
      961 agccccagat atgcgttatt caccagcaca tggatgtacg gaatgttgaa ctgcgcgcca
     1021 acggctaact cttcaatcag gaactggaag tcaaagtcgc cagaaatcgc caccacattg
     1081 cgctctggat cagcggcaca aacccccagc gccgccggaa tcgtccagcc taacggacca
     1141 gcctgaccac agttgatcca gtggcggtct ttaaagacat gcagcatttg tgccgcagcg
     1201 atttgtgaca ggccaatggt ggtgacataa caaacatcgc gaccaaaggc tttgttcatc
     1261 tcttcataca cgcgctgcgg tttcaccggc acgttgtcga aatgggtttt gcgcagcaaa
     1321 gtgcgtttgc gctgctggca gtcggcgacc cattctttac gacacggcag acgacccgct
     1381 ttttgcatct cctgcgccac ttcaaccagc agtgtcagcg ccgctttagc atcagagaca
     1441 atgccgagat ccggacacag cacgcggcca atttgcgtcg gctcaatatc gatatgaacg
     1501 attttgcgcc cttcggtgta tttctctacc gaaccggtat gacggttagc aaaacggtta
     1561 ccgataccaa acaccatgtc ggacgccagc aacgttgcgt taccgtaacg atgcgcggtt
     1621 tgcagaccga ccatcccggc catcagttca tgatcgtccg ggatacagcc ccagcccatc
     1681 agcgttggga tcaccggaac gctggtcagt tcagcaaact gttgtaacag tgcagctgcg
     1741 tcagcattga ttaccccgcc cccggcaaca atcaccggac gttcggcctg aattaacatt
     1801 tctacagctt tttcgatctg cgtacggctg gcagcaggtt tgtagaccgg cagcggttcg
     1861 tacatgtcag gatcaaactc gatttccgca acctgaacgt cgaacggtaa atccaccagt
     1921 accggccccg gacgaccaga acgcatcagg tgaaatgcct gttgcagcac gcgaggcacc
     1981 agcgccgctt cacgaactgt aaccgccatt ttgctgaccg gtttagcaat tgcttcaata
     2041 tctacggcct gaaaatcttc tttatgcaga cgggcgcgcg gtgcctggcc ggtaatgcac
     2101 agaataggaa tggaatcagc agaagcggaa tagagcgcgg tgatcatgtc cgtgcccgca
     2161 ggaccggaag tccccagaca tacgccgata ttacctgccg ttgcgcgggt ataaccttcc
     2221 gccatgtgcg aagcaccttc cacatgacgc gccagaatgt gacgaatacc gccgtgctta
     2281 cgcatcgctg agtagaacgg attgattgca gctcccggaa caccaaaggc ggtggtgata
     2341 ccttctttct ccagcacata cattgccgcg tcaacggctc tcatttttgc cattttattc
     2401 ctacctctat taattggaaa aattttccaa ctttcaaatt ttgtcaaaaa aacccgctcc
     2461 gcagagcggg atgcgacaga cactatggat gtgctttcag tcccaacgcc gtgctgatat
     2521 cgcgggcggt gtctctgacc agctcaccct gactgacaaa acgatcttct gtcagtcttg
     2581 atgaaggccc ggagatggag atagcggcaa caacactacc tacatcatcg taaattgctg
     2641 aagctatgca attcagacct acaacatgct cttctttatc tacggtatag cccagttcac
     2701 gcgcttgctc caggtccttc agtaaggtgg gcatatccac aagcgtagtt ggcgtaaact
     2761 gctgcaaacc ggtttgcaga atgatgctca tcaactcctc ttccgccagc ggataaagca
     2821 gcgctttgcc cgcaccggaa gcatgcagtg gcagacgact gcccagtggc gcacacatcc
     2881 tgaccatcga tttacactct aactgaccaa ttaataccgc ttcattgccg ttacggatcg
     2941 cgacattgac cgtttcgccg gaaagtaaca tcagacggcg cataaacggc ccggcgacgg
     3001 agaggacatc gcggttatgg atgtacgctg caccgacgtt aaagacacct aatcctatat
     3061 gccaccagcc taactgactg tcctgataga caaaatccgc tgcctgtaaa accttcagca
     3121 agcgaaaggt cgtggagagc ggcaaatcca gattgagaga aatatcgcta accgaagaac
     3181 ttcccccact tttttccaga tattgcagaa tcgcaattcc ccgctctaat gcctgtgcgc
     3241 ccttctgtgc cacaggctcc gcctgtcctg gcctgccgcg ccgtctaact tccgtcatac
     3301 ctacgttaac tccgacctac aagtgagcat aaacaatgaa caattagttt atcttatgca
     3361 agtcggttgc aggcgtcacg caaaacagag ttcctgttca ggaatacttt caacatcaca
     3421 gttgtcactg ccgccgcgat cgatggtcag aaaatcggtg acgtgctgcc aggcgaaaag
     3481 tgggtgatgc cagacgttac gatggtaatt cactccctgt tcgccgttgg taataaacgc
     3541 ccgcagggtt gacaggtctg gtttgtcgtc acctaacgcc acgaccacca caaacacctc
     3601 acctttcatc gggataaagg cctgagtacc cagcggatga cgttcgagtt cgtgaatggt
     3661 cagcggcaga ttcgccggtt gcgcgcggtt aatgctgata agcgtacggt cttgctcaag
     3721 aatctcaacc agcgccaaat cgtggtaacg ctccaccagg ccattgttaa tatggaaaaa
     3781 atcccgttgc tgcgtttcga ttacgtcgcc ataagcacta aaggcttcct gacttaacgg
     3841 taatacctga agtttcatct ttttgtcctt gtaacataat tccgttcaac caccgccgac
     3901 cggtcagctt ggtttcaata acaggcaatc taaaacctta agagaacaac atcaacacaa
     3961 aagttggaat atttttccaa aactagattt agtccacaaa ttgaacaggg ttatcatgta
     4021 aaaatactga taaatcagag gataagaaaa tcacgcaact tgcggattta aaaggggata
     4081 cctgaagatg ttcgatccag aaaccttgcg gactttcatt gcggttgctg aaacaggaag
     4141 tttttcaaaa gcggcagaac gattatgtaa aaccacggcg acgatcagtt atcgcattaa
     4201 acttctggaa gagaataccg gagtagcgct gtttttccgt acgactcgca gcgtgacgtt
     4261 gacagcggct ggcgagcatc tactttccca ggccagagac tggctgagct ggctggaaag
     4321 tatgccaagc gagctgcaac aggtgaatga tggcgtggaa cgccaggtga atattgtcat
     4381 caacaacctg ctctacaacc cccaggccgt cgcccagttg ctcgcgtggc tgaatgagcg
     4441 ttaccccttt acccagtttc acatctcccg acaaatctat atgggcgtct gggactcgct
     4501 attgtacgaa ggtttttcgc tggctatcgg cgtcacggga actgaggcgc tggcaaatac
     4561 ctttagtctt gatcccttag gatcggtgca atggcgattt gtcatggcgg cggatcatcc
     4621 gctggcgaac gttgaagagc cgctaacaga agcgcagttg cggcgctttc cggcggtcaa
     4681 tattgaagac agcgcccgca ccttaaccaa acgcgtcgcc tggcgattgc cagggcaaaa
     4741 agagattatt gttcctgata tggaaacgaa aatcgccgcc catctggcgg gcgttggcat
     4801 tggttttttg ccaaaatcgc tttgccagtc aatgatcgat aatcaacaac tggtcagccg
     4861 ggtaatccca acgatgcgcc ctccttcgcc attgagtctg gcatggcgca aatttggcag
     4921 cggcaaagcg gtagaagata ttgtgacctt gtttacccag cgcaggccgg aaatcagcgg
     4981 atttttagaa attttcggca acccacgcag ttaaaaatca agtttccttg cgcgttggcg
     5041 cgtaaaatag cgcgcaagct gatttgaggt tcacttttaa ctatgcaaga gagacacacg
     5101 gaacaggact atcgtgccct gctgattgct gatacgccca ttattgatgt tcgcgcccct
     5161 atagagtttg agcaaggcgc aatgcccgcc gctatcaatc tgccgttaat gaataacgat
     5221 gaacgcgccg ccgttggcac ctgctataaa cagcaaggct cagacgcagc gctggcgctg
     5281 ggacataaac tggttgcggg tgaaattcgt cagcagcgca tggacgcctg gcgggcagcg
     5341 tgcctgcaaa atccgcaagg tattctctgc tgcgcccgtg gcggtcagcg ctcacatatt
     5401 gtgcaaagct ggttgcatgc agcggggatt gattatccgc tggtggcagg cggttataag
     5461 gcactgcgcc agaccgcgat tcaggcgact attgaactgg cacaaaaacc gatagtgctg
     5521 attggcggtt gtaccggcag cggtaaaacg ctgttagtgc agcaacagcc gaacggtgtt
     5581 gatctggaag ggttggcgcg tcatcgcggt tcggcgtttg gtcgcacgtt acaaccacaa
     5641 cttagccagg cgagttttga aaacctgctg gctgccgaaa tgctaaaaac cgatgcccgt
     5701 cagaatttgc gcctgtgggt gctggaagac gaaagccgga tgatcggttc gaatcacctg
     5761 ccggaatgcc tgcgcgagcg aatgactcag gcggcgattg cggtggtaga agatccgttt
     5821 gagatccgtc ttgagcgcct gaacgaagag tatttcttgc gtatgcatca tgattttacc
     5881 cacgcgtacg gcgacgaaca gggctggcag gaatattgcg aatacctgca tcacggactt
     5941 tcggctatta agcgtcggct ggggctacag cgatataacg aactggctgg aaggctggat
     6001 gcagcactga caacgcaact caccaccggc agcaccgacg gtcatctggc ctggctggtg
     6061 ccgttacttg aagaatatta cgacccaatg tatcgctatc agcttgagaa aaaagcggaa
     6121 aaagttgtct ttcgcggtga gtgggcggaa gtggcggaat gggttaaggc gcagtaatga
     6181 gggacgtatt tgtattagtc gcgacttgat ctgacacata gccttgaaag gttgagagtt
     6241 accggttttg atatgagtgt cgaatcctta tccaaaacaa gaggtaactc tcatgcttca
     6301 tactgccaat cccgtcatca aacacaaagc cggtttgctc aatctggctg aagaactcag
     6361 caacgtatca aaagcttgta aaatcatggg cgtttcgcgt gatacgtttt atcgttaccg
     6421 cgaactggtc gctgaaggcg gtgtggatgc gcagattaat cgtagtcgcc gcgctcctaa
     6481 ccttaagaac cgtaccgatg aggcaactga acaggctgtt gttgattacg ccgtcgcttt
     6541 cccgacacat ggtcagcacc gggccagcaa cgagctgcgt aaacaggagg tttttatctc
     6601 cgacagtggt gtccgttctg tctggttact ccataacctt gagaatctca aaagacgata
     6661 ctgaacaaat tttatcaggt gacgttccgt aaaaagttgt atggagttct cgatgcagtc
     6721 ggcgcgacta tcagtacccc cgctgaaccc tggactatcg ggccgccgta ctggggcatg
     6781 tcaccctggc gtgccgctgg ttttccgcta atggtttatt tcctctttct ttcccagcaa
     6841 gaatttattt taatccggaa ggattattta ttttcagata gggactcaca ttaaacacgt
     6901 aacattttaa ttaatgcatg gctgaatatg tattacacaa tgtcagatat cgtaaaagac
     6961 cagtgcaatt ctatgtaaac tctctgactg ttcattttat ttgttgtttc agggtcggaa
     7021 ctcaccctca ataaacaact gaatacgagt agatattcat cgtctgagct atatggcttt
     7081 acacaatagc cgacatgtct tttctcagtc aggaatttta cttttcctca ctacgaagtt
     7141 gacgacttcg atatgggata gactcttaat tcaagcaatt actctgcaca agcatcaggc
     7201 aatccagctc caatatttgt tataaaataa tgcgtaacat taatttcacc aacaaaaaaa
     7261 aggtttacgc tatccccttg ccctggtatt ttaccatatt tacaccacac tttttttgta
     7321 tctggatcat tctctgatac gattttc
//
LOCUS       DAPGOF010000212         7321 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.213, whole genome shotgun sequence.
ACCESSION   DAPGOF010000212 DAPGOF010000000
VERSION     DAPGOF010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.213"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1734
                     /gene="adeD"
                     /locus_tag="RHV13_004215"
     CDS             <1..1734
                     /gene="adeD"
                     /locus_tag="RHV13_004215"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDV1243678.1"
                     /translation="ISRAEYQELLAVSRGDAVADYIIDNVSILDLINGGEISGPIVIK
                     GRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVTFETATLPRGLT
                     TVICDPHEIVNVMGEAGFAWFARCAKQARQNQYLQVSSCVPALEGCDVNGASFTLEQM
                     LAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGLGGKELNAYIAA
                     GIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNSPQCMLCTDDRN
                     PWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGLLAPGKQADIVL
                     LSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQPVSASDFALQFT
                     PGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRLAPACGLLGGFG
                     LNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNGQVQSHLPLPIA
                     GLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPALKLTSQGLFDGE
                     KFAFTTLEVTE"
     gene            complement(1777..3168)
                     /gene="uhpT"
                     /locus_tag="RHV13_004216"
     CDS             complement(1777..3168)
                     /gene="uhpT"
                     /locus_tag="RHV13_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HDV1243679.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNQYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3306..4625)
                     /gene="uhpC"
                     /locus_tag="RHV13_004217"
     CDS             complement(3306..4625)
                     /gene="uhpC"
                     /locus_tag="RHV13_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HDV1243680.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLDVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQAPREA"
     gene            complement(4635..6137)
                     /gene="uhpB"
                     /locus_tag="RHV13_004218"
     CDS             complement(4635..6137)
                     /gene="uhpB"
                     /locus_tag="RHV13_004218"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HDV1243681.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFSLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPAELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6137..6727)
                     /gene="uhpA"
                     /locus_tag="RHV13_004219"
     CDS             complement(6137..6727)
                     /gene="uhpA"
                     /locus_tag="RHV13_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HDV1243682.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6889..>7321)
                     /locus_tag="RHV13_004220"
     CDS             complement(6889..>7321)
                     /locus_tag="RHV13_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243683.1"
                     /translation="SISGIGNLYYACSQAFPVALDRGDINAIQANDSVSAGLNNNPGF
                     CALLGKTNFLVADFSQHTFTQTHYIVKAEINEAGNDFNIANFIYRTYGEDYIHNDSVD
                     DSLNERSAEDYRFLDDSLLPENDFRNRRRNFSGNIAIATYK"
BASE COUNT         1870 a         2059 c         1890 g         1502 t
ORIGIN      
        1 attagccggg ctgaatacca ggaattgtta gccgtttccc gtggcgacgc tgttgccgat
       61 tatattattg ataatgtctc tattctcgac ctgatcaatg gcggagaaat ttccggccca
      121 attgtgatta aaggacgtta cattgccggt gttggcgcag aatacgctga tactccggct
      181 ttgcagcgga ttgatgcccg cggcgcaacg gcggtgccag ggtttattga tgctcacctg
      241 catattgaat ccagcatgat gacgccggtc acctttgaaa ccgctactct gccgcgcggc
      301 ctgacgaccg ttatttgcga ccctcatgaa atcgtcaacg tgatgggcga agccggattc
      361 gcctggtttg cccgctgtgc aaaacaggca aggcaaaacc agtacttaca ggtcagctct
      421 tgcgtgcccg ctctggaagg ctgcgatgtt aacggtgcca gttttaccct tgaacagatg
      481 ctcgcctggc gggaccatcc gcaggttacc ggccttgcag aaatgatgga ctaccctggc
      541 gtaattagcg ggcagaatgc gctgctcgat aaactggatg catttcgcca cctgacgctg
      601 gacggtcact gcccgggttt gggtggtaaa gaactcaacg cctatattgc cgcgggtatt
      661 gaaaactgcc acgaaagtta tcagctggaa gaaggacgcc ggaaattaca actcggcatg
      721 tcgttgatga tccgcgaggg gtccgctgcc cgcaatctca acgcactggc accgttgatc
      781 aacgaattta acagcccgca atgcatgctc tgtactgatg accgtaaccc gtgggagatc
      841 gcccatgaag ggcatatcga tgccctaatt cgccgcctga tcgaacaaca caatgtgccg
      901 ctgcatgtgg catatcgcgt cgccagctgg tcgacggcac gccactttgg tctgaatcac
      961 ctgggcttac tggcacccgg taagcaggct gatatcgtcc tgttgagcga tgcgcgtaag
     1021 gtcacggtgc agcaggtact ggtgaaaggc gaaccgattg acgcgcaaac cttacaggcg
     1081 gaagagtcgg cgagactggc acaatccgct ccaccgtatg gcaataccat tgcccgccag
     1141 ccagtttccg ccagcgactt tgccctgcaa tttacacccg gaaaacgcta tcgggtcatt
     1201 gacgtcatcc ataacgaatt gattacgcac tcccgctcca gcgtctacag cgaaaatggt
     1261 tttgatcgcg atgatgtgtg ctttattgcc gtacttgagc gttacgggca acgtctggct
     1321 ccggcctgtg gtttgctcgg cggctttggc ctgaatgaag gtgcgctggc tgcgacggtc
     1381 agccatgaca gccataatat tgtggtgatc ggtcgcagtg ccgaagagat ggcgctggcg
     1441 gtcaatcagg tgattcagga tggcggcggg ttgtgcgtgg tacgtaacgg ccaggtgcaa
     1501 agtcatctac cgttgcccat tgccgggctg atgagcaccg acacggcgca gtcgctggcc
     1561 gagcaaattg acgccttgaa agccgccgcc cgtgaatgtg gtccgttacc ggatgagccg
     1621 tttattcaga tggcgtttct ttctctgcca gtgatccccg cgctgaaact aaccagtcag
     1681 gggctatttg atggcgagaa gtttgccttc accacgctgg aagtcacgga ataaaaaaag
     1741 cccggcgtca tgccgggcaa aagtcaccag ttacgtttat gccactgtca actgctgaat
     1801 ttttttctcg cggcggattt tgcgttcttc catcaccgcc actatcgcca tcaggcagat
     1861 acaaccaatc gcggcgatat ccagcgcggc gaaggtgcct gcccagccgg taaggccgaa
     1921 taccggcgtc ccatcggcaa tcatccccag acctaacttg gcaaagctgt caccaatcag
     1981 gtaagcaaag gtgcctttaa taccatcggc agcgccaatc gcttttttag gtacaaagcc
     2041 aacagcagcc acaccaatca acaattgcgg gccaaagacc aggaaaccca acgcaaagag
     2101 agaagccaga tagatatatt gattactggc atgttgatac acaccgagcg tggcgataat
     2161 cagcgccagc gcgatgcagg ccaccaggcc acggcgaccg ttcgccaggt cagagagcca
     2221 gccccacagc agcgtaccga ccagcgcacc agcttcaaac agcgtaaagc cctgaatcgc
     2281 caccgcttta gagagtttca gttcctggaa cgcgtatacg gttgaccact ggtcgatacc
     2341 aatacgtacc acatagagga aaatgttggc gaagcacagc agccagatca ctttgttttt
     2401 cagcacatac tcaacaaaga tctgccactt ggtcatatcg gtagattctg tctctttgtc
     2461 ctcttcgctg atctcctcgc cgaacagttc ttcagctttg ccgaggccat aagattccgg
     2521 ggagtcgctg ccgtaacgca ggccgataaa accgacaatc agcgcgataa tcgacgggaa
     2581 gataaacatg ccgatgacat ggccatcgaa caggtaattt gcgccgaaca gcgctacacc
     2641 tgccgcgcct gcaccgccaa ggttgtgaga aatgttccag aaaccgagga aggtcccgcg
     2701 cttacgacgc ggcgtccatt tggtgatggt ggagtaactg cacgaaccgc cggtgctctg
     2761 gaaaaagccg cttaaggcgt agaaggcaat catcaggaac aggctaaccg agccgctgcc
     2821 catactggcg ctgaagccca gcatacaaat ggcagacagg atcagcatga acggcaggaa
     2881 ttgtttggtg tttttgccgt cggcgtagta ggaaaccagc gttttaccca cgccataagt
     2941 gatggagaag cccaggccga tcatccccag ctgcgtcatg ctcaacccgt aggtcgaaat
     3001 catatcgttc tgcgcgatgt taaagttctt gcgaatcagg tacatcgtca ggtagccgat
     3061 aaagaccacc aggtaggatt gcatgaacgg tttgaaccac attttgcgcc gcacttcgag
     3121 cggaaggtcc agggtcggct tgcggacctg gtttaagaaa gccagcatgg gttactcctg
     3181 aaatgaatac ctgcgtgagg catgcattgt aaaaatcagc agcaaatcag gcctgagata
     3241 gcgtccaggc aaaacctgga aaatttctta gttttgccgg atatgaagtg aaaaggtgag
     3301 atgcatcacg cttcgcgcgg tgcctgggcg ttcaaaaagg gcagtaacag cagtgcggaa
     3361 atcccggcgg cgatagagat aaccacaaaa aatccgctcc agtgccaggt atcgagtact
     3421 ttcgccagtg gccaaccagc aagcgacgcc cccagataag caaacaagcc gacaaacccc
     3481 gtcgccgccc ctgccgcctc tttgtgggaa cactctgccg ccgccatgcc gattaacatc
     3541 tgcgggccaa agacaaaaaa accaatagtg aagaagcagg ttgcctgcat cacgtagctg
     3601 gcaaatggca tcagccacag cgagccgact gaaagcaaaa ttccggcggc gaaaatcaaa
     3661 ttcatcggcc cacggttgcc gttaaacaat ttgtccgagc cccaaccggc taccagcgca
     3721 ccgataaatc cgcccagttc aaacatcgtc actgccgtat tcgccgtgac cagatcgacg
     3781 tccagcgtct cggacatata caaattgccc cagtcgttga ttgccgcccg gaccacatag
     3841 accagcacat agcaaaacga aagcagccag atatacggat tcagtaacac atacttggtg
     3901 aggatctctt tacgcgtcag ccctgcccct tcttgttgtt gagcaatctc cagcgcgtcg
     3961 tgccgccatt caccgaccgc cggtaaacct aacgcctgcg ggcgatcgcg cagccgccag
     4021 cagagaaaaa tccccacgac gatcgccata caaccagcaa tcatcatccc ggcacgccag
     4081 ccgtaatgta gtgcagccgc tgccatcaca atgggaatga gtgcgccgcc gacgttatgc
     4141 gccgtgttcc ataatgccca ccaaccgccg cgctcggtac gagaatacca ggcggttaat
     4201 agacgcgcac acaccggtga accccagccc tggaaaaagg cgttcagcac ccagagtacg
     4261 gcaaacgccc atagagacgt cgagaagcca aacagaatgt tgataatgcc cgtggcgata
     4321 agccctatcc ccataaaata acgggcattt gagcgatcgc tgacaatgcc ggagacaaac
     4381 ttcgacacgc cgtaagtaat gtaaaacagg gtcgctaaca ggccgatatc gctacggctg
     4441 agcacgccgt tagcaaggat ttctggtacg gcggcgttaa aacttttccg cgtgaagtaa
     4501 aatagcgcgt aacccagcca gatggtcagc agaatatgcc gacgccagta gcgatagcgt
     4561 gcatcaattt catgtttgtc agtcattaat ggcgcatcgg caggcgcttt cagaaacggc
     4621 aacatcatcg aaccttagac ataacgttga ggtagagaaa cgctgacacg cgtgccgtgc
     4681 agacaggaaa tgtgtaatgt gccgcccagc gccgttacgc gctcgcgcat gccggtgagg
     4741 ccaaaacctt gttgcccgga acccggcggc aaaccgctgc catcgtcttc aatcaccagc
     4801 atcaaccgtt catcctgctg ccagccttgc agcgtaaccg cgctggcatc ggcatgtttc
     4861 acaatgttgt tcagcccttc ctggcagacg cgaaacagcg tcacgcgctg gttttcactt
     4921 aacgctgact catcgatacg ccattcgaga tggctgacaa tgccgcgccc ttccagctcc
     4981 atttcccgca tcagtgagcg gatggcctgc tccagggtga gatcatccag ctggcgcgga
     5041 cgtaaccgcc ctaacaaacg gcgcaccgca tcgtaaacac ccagcgacag ttgttcgatg
     5101 agctgcccgc tctgcttcac gctggcgtta tctgccgcca gtcgctggac aatgcccgcc
     5161 tgagtacgaa tggcagtgat ggtctgacct atatcatcgt gcagctcacg cgccacatca
     5221 cggcgcacgc tctcttcggt ttctaacaaa cgttcagcca gatgctgatt gcgcgccagt
     5281 tctttttgca gcgactggtt aagttcacgc aaccgctgga tgccagcgcc aagcaacaac
     5341 cctgtcagac tttgcaccag cagcgagagc aataagtcca ccggatgatc gcgccaggtt
     5401 tgactggcga tcagcgcgat ggcgttcatc aacgtcgcaa tcagcgcccc ttgccaacca
     5461 tagtgccagg ccagcgcgat aatcggcagc gccagacaga atggcgtaaa gcgcgacagt
     5521 tcggccggca atcccaactg gagccagaga ctgataacaa acagcagcaa gtaccagacc
     5581 agatgtcgcc cgcgccagtt gatcggttga gaaactaacg acgggccgag cggcagccag
     5641 gtgttattgg cgagatagtg ccagaacacc agacatatag gggccagcgt caggccgcca
     5701 gttaaagtca gtagtagcgc attccacgac tctttgccgt gccaaagcca gggcagcgac
     5761 tgcaacaacg ccgccgccgt cagggccgcc ccctgtaaca gcaaggtgcg ccagtcacgt
     5821 tgatggcgat agcgcgagat cagcgcaacg ggtagtaacg tcagtaaact accgatcatc
     5881 aataaggaaa aatgggttaa accgaccgcc tgcgttagcc agtaaatcag cagccactcc
     5941 gcgcccagca gcaccggcca gtatccgcgc gggcattgca gcattagccc cagacgcaga
     6001 ccaaacggaa ataacagcac cgccatatca gggcgctcaa ccaggtgcag gctgatactc
     6061 cacaagcaaa accatgcggc agagaagata aaaaagcagg caataacggt aattaagcgg
     6121 gagaacaacg tcttcatcac cagccatcaa acatgcggcg cgccagttca acgtcgttac
     6181 tgacacccag tttttccatc aggttggcac gatgtacatg cacagttttt ggcgacaaac
     6241 ctagttcggc ggcaatctct ttcaccgcca ttccttgcgc cagtttttcc gccacctggc
     6301 gttcacgttt ggttagcggg tcctggcgac cggatgccag tttaatggca atatccggcg
     6361 tcagataaca gccgcccgtg gcaaccgtat gcaccgcagc aatcagttcg tcagggctac
     6421 aacgtttgga gagaaagccg cgcgcccccg cgttaagcgc ctgctcaacc agcgccggac
     6481 tgtcatgaac ggagagcata attgtcgcca tacctttcgg cagttggctt agcaactcca
     6541 gaccggaaat atcaggcatg gagatatcgc aaatacacac ctgcacaccg cgccccggca
     6601 accccgccag cgcctcgcgc cccgaaccaa actcggcaac tacttgcaaa tcaggttcca
     6661 gccccagcaa ctgcgcaaag ccggagcgga cgatgaggtg atcgtctata agggcaacgg
     6721 tgatcatgat tgggtcctgg cggataaaaa acgcgcatac cttaacgata agcgcgatgt
     6781 tgttcaagtt ggatatgggg tatgtattgg cctaatgatg ttgcgcttat cgtcaaaacg
     6841 cttacctgaa aggtaagcgt ttggtgcgtg ttattgagaa aaagcgaatt atttgtaggt
     6901 tgcgattgcg atattgcctg aaaaatttct cctcctgttg cggaaatcgt tttcaggtaa
     6961 caaagagtca tccagaaacc tataatcttc agcggaacgc tcattaagag agtcatccac
     7021 tgagtcgtta tgaatataat cctcaccgta tgttcgataa atgaaattag caatattaaa
     7081 atcgttacca gcttcattta tttcggcttt tacaatataa tgagtttgtg tgaatgtatg
     7141 ctgtgaaaaa tcagcaacaa gaaaatttgt ttttcccagc aaagcacaaa accctgggtt
     7201 attattaagc ccggcagaaa cagaatcgtt tgcctgtatt gcattaatat cccccctatc
     7261 aagcgcgaca ggaaatgcct gtgaacaagc ataatacagg ttacctattc ctgaaatact
     7321 a
//
LOCUS       DAPGOF010000213         7284 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.214, whole genome shotgun sequence.
ACCESSION   DAPGOF010000213 DAPGOF010000000
VERSION     DAPGOF010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.214"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            82..197
                     /gene="rrf"
                     /locus_tag="RHV13_004221"
     rRNA            82..197
                     /gene="rrf"
                     /locus_tag="RHV13_004221"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(316..759)
                     /locus_tag="RHV13_004222"
     CDS             complement(316..759)
                     /locus_tag="RHV13_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDV1243684.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
     gene            916..1845
                     /gene="metA"
                     /locus_tag="RHV13_004223"
     CDS             916..1845
                     /gene="metA"
                     /locus_tag="RHV13_004223"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HDV1243685.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQDQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEFFRDVEAGLDPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            2114..3715
                     /gene="aceB"
                     /locus_tag="RHV13_004224"
     CDS             2114..3715
                     /gene="aceB"
                     /locus_tag="RHV13_004224"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HDV1243686.1"
                     /translation="MTEQATTTDELAFIRPYGEQERQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDTDWKIRGIPADLQDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            3745..5049
                     /gene="aceA"
                     /locus_tag="RHV13_004225"
     CDS             3745..5049
                     /gene="aceA"
                     /locus_tag="RHV13_004225"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HDV1243687.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVDKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            5330..7066
                     /gene="aceK"
                     /locus_tag="RHV13_004226"
     CDS             5330..7066
                     /gene="aceK"
                     /locus_tag="RHV13_004226"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HDV1243688.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAEFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFYPEHGWEALLMRVLSDL
                     PLRLRWQNKSRDINYIIRHLKETLGPENLSESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDRFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(7035..>7284)
                     /locus_tag="RHV13_004227"
     CDS             complement(7035..>7284)
                     /locus_tag="RHV13_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243689.1"
                     /translation="DHARLFGFTAEDIMDFWQHKAPQKYSAFELACELGHRVIAELIF
                     NTLNKMAESFGFTDNPRYIAEKNYMEALLKKASPHTVR"
BASE COUNT         1776 a         1884 c         1884 g         1740 t
ORIGIN      
        1 tgttttggcg gatgagaaaa gattttcagc ctgatacaga ttaaattaac cggcggtaag
       61 cgggttaata aacagaattt gcctggcggc cttagcgcgg tggtcccacc tgaccccatg
      121 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggtctcc ccatgcgaga
      181 gtagggaact gccaggcatc aaatttagcg aaaggccacc cgtcaggatg gcctttttgc
      241 attggcgcag aaaaaaatgc cggatgcgac gctggcgcgt cttatccagc ttacgcaggc
      301 acgatagggg gcagcttatt cccccacata cgccagattc agcaacggat acggtttccc
      361 caaatcgtcc acctcagagc gtcccgtaac cttaaaaccc accttcttat agaacccaac
      421 cgcctgctca ttttgctcat taacgttggt tgtcagttcc ggtgccatcg agagcgcatg
      481 ctccaccagc atccgaccca cgccgcagcc gcacacatca ggatcgataa acagcgcatc
      541 catatgctgc ccacttaaca acataaaccc aaccggctga tcccgttcat taaccgcgac
      601 ccacaacggt gcttccggca gaaaggaacg aaccagctct tccagctcgg cccgatactc
      661 tgctgataaa aaatcgtggg tggcatcgac agatcgacac caaatcgcaa cgagttcctc
      721 cccttcctca tgccgtgagc ggcgaatact aataaccatt ttctctcctt ttagtcattc
      781 ttatattcta acgtagtctt ttccttgaaa ctttctcacc ttcaacatgc aggctcgaca
      841 ttggcaaatt ttctggttat cttcagctat ctggatgtct aaacgtataa gcgtatgtag
      901 tgaggtaatc aggttatgcc gattcgtgtg ccggacgagc tacccgccgt caatttcttg
      961 cgtgaagaaa acgtctttgt gatgacaact tctcgtgcgt ctggtcagga aattcgtcca
     1021 cttaaggttc tgatccttaa cctgatgccg aagaagattg aaactgaaaa tcagtttctg
     1081 cgcctgcttt caaactcacc tttgcaggtc gatattcagc tgttgcgcat cgattcccgt
     1141 gaatcgcgca acacgcccgc agagcatctg aataacttct actgtaactt tgaagatatt
     1201 caggatcaga actttgacgg tttgattgta actggtgcgc cgctgggcct ggtggagttt
     1261 aatgatgtcg cttactggcc gcagatcaaa caggtgctgg agtggtcgaa agatcacgtc
     1321 acctcgacgc tgtttgtctg ttgggcggtc caggccgcgc tcaatatcct ctacggcatt
     1381 cctaagcaaa cccgcaccga caaactctct ggcgtttacg agcatcacat tctccatcct
     1441 catgcgcttc tgacgcgtgg ctttgatgat tcattcctgg caccgcattc gcgctatgct
     1501 gactttccgg cagcgttgat tcgtgattac accgatctgg aaattctggc agagacggaa
     1561 gaaggggatg catatctgtt tgccagcaaa gataagcgaa ttgcctttgt gacgggccat
     1621 cccgaatatg atgcgcaaac gctggcgcag gaatttttcc gcgatgtgga agccggacta
     1681 gacccggaag taccgtataa ctatttcccg cacaatgatc cgcaaaatac accgcgagcg
     1741 agctggcgta gtcacggtaa tttgctgttt accaactggc tcaactatta cgtctaccag
     1801 atcacgccat acgatctacg gcacatgaat ccaacgctgg attaatcttc tgtgatagtc
     1861 gatcgttaag cgattcagca ccttacctca ggcaccttct ggtgcctttt ttatttccga
     1921 aacgcacctc aggaggtgaa taaattttat tcatattgtt atcaataagt tatcaagtat
     1981 ttttaattaa aatggaaatt gtttttgatt ttgcatttta aatgagtagt cttagttgtg
     2041 ctgaacgaaa agcgcacaac gatccttcgt tcacagtggg gaagttttcg gatccatgac
     2101 gaggagctgc acgatgactg aacaggcaac aacaaccgat gaactggctt tcataaggcc
     2161 gtatggcgag caggagaggc aaattcttac tgccgaagcg gtagaatttc tgactgagct
     2221 ggtgacgcat tttacgccac aacgcaataa acttctggca gcgcgcattc agcagcagca
     2281 agatattgat aacggaacgt tgcctgattt tatttcggaa acagcttcca ttcgcgatac
     2341 agactggaaa attcgtggta ttcccgcgga cttacaagat cgtcgagtcg agataactgg
     2401 cccggttgag cgcaagatgg tgatcaacgc actgaacgcc aatgtgaaag tctttatggc
     2461 cgatttcgaa gattcactgg caccagactg gaacaaagtg atcgacgggc aaattaacct
     2521 gcgcgatgcg gttaacggca ccatcagtta cacgaatgaa gcaggcaaaa tttaccagct
     2581 caagcccaat ccagcggttt tgatttgtcg ggtacgcggt ctgcacttgc cggaaaaaca
     2641 tgtcacctgg cgtggtgagg caatccccgg tagcctgttt gattttgcgc tctacttctt
     2701 tcacaactac caggccttac tggcaaaggg cagtggtccc tatttctatc tgccgaaaac
     2761 ccagtcctgg caggaagcgg cctggtggag cgaagtcttc agctatgccg aagatcgctt
     2821 taatctgccg cgcggcacca tcaaggcgac gttgctgatt gaaacgctgc ccgccgtgtt
     2881 ccagatggat gaaatccttc acgcgctgcg tgaccatatt gttggtctga actgcggtcg
     2941 ttgggattac atcttcagct atatcaaaac gttgaaaaac tatcccgatc gcgtcctgcc
     3001 agacagacag gcagtgacga tggataaacc attcctgaat gcttactcac gcctgttgat
     3061 taaaacctgc cataaacgcg gtgcttttgc gatgggcggc atggcagcgt ttattccgag
     3121 caaagatgaa gagcgcaata accaggtgct caacaaagta aaagcggata aagcgctgga
     3181 agccaataac ggtcacgatg gcacatggat tgctcaccca ggccttgcgg atacggcaat
     3241 ggcggtattc aacgacattc tcggctcccg taaaaatcag cttgaagtga tgcgcgaaca
     3301 agacgcgccg attactgccg atcagctgct ggcaccttgt gatggtgaac gcaccgaaga
     3361 aggtatgcgc gccaacattc gcgtggcagt gcagtacatc gaagcatgga tctccggcaa
     3421 cggctgcgtg ccgatttatg gcctgatgga agatgcggcg acggctgaaa tttcccgtac
     3481 ctcaatctgg cagtggatcc atcatcaaaa aacgttgagc aatggcaaac cggtgaccaa
     3541 agccttgttc cgccagatgc tgggtgaaga gatgaaagtc attgccagcg aactgggcga
     3601 agaacgtttc tcccaggggc gttttgacga tgccgcacgc ttgatggaac agatcaccac
     3661 ttccgatgag ttaattgatt tcctgaccct gccaggctac cgcctgttag cgtaaaccac
     3721 cacataacta tggagcatct gcacatgaaa acccgtacac aacaaattga agaattacag
     3781 aaagagtgga ctcaaccgcg ttgggaaggc attactcgcc catacagcgc ggaagatgtg
     3841 gtgaaattac gcggttcagt caatcctgaa tgcaccctgg cgcaactggg ggcggcgaaa
     3901 atgtggcgtc tgctgcatgg cgagtcgaaa aaaggctaca tcaacagcct cggtgcactg
     3961 actggcggtc aggcgctgca acaggcgaaa gcgggtattg aagcagtcta tctgtcggga
     4021 tggcaggtag cggcggacgc taacctggcg gccagcatgt atccggatca gtcgctctat
     4081 ccggcaaact cggtgccagc tgtggtggag cggatcaaca acaccttccg tcgtgccgat
     4141 cagatccaat ggtccgcagg cattgagccg ggcgatccgc gctatgtcga ttacttcctg
     4201 ccgatcgttg ccgatgcgga agccggtttt ggcggtgtcc tgaatgcctt tgaactgatg
     4261 aaagcgatga ttgaagccgg tgcagcggca gttcacttcg aagatcagct ggcgtcagtg
     4321 aagaaatgcg gtcacatggg cggcaaagtt ttggtgccaa ctcaggaagc tattcagaaa
     4381 ctggtcgcgg cgcgtcttgc agctgacgtg acgggcgttc caaccctgct ggttgctcgt
     4441 accgatgccg atgcggcgga tctgattacc tccgattgcg acccgtatga cagtgaattt
     4501 attactggcg agcgtaccag tgaaggcttc ttccgtactc atgcgggcat tgagcaagcg
     4561 atcagccgtg gcctggcgta tgcgccatat gctgacctgg tctggtgtga aacctccacg
     4621 ccggatctgg aactggcgcg tcgctttgca caagccatcc acgcgaaata tccgggcaaa
     4681 ctgctggctt ataactgctc gccgtcgttc aactggcaga aaaacctcga cgacaaaact
     4741 attgccagct tccagcagca gctgtcggat atgggctaca agttccagtt catcaccctg
     4801 gcggggatcc acagcatgtg gttcaacatg tttgacctgg caaacgctta tgcccagggc
     4861 gagggtatga agcactacgt tgataaagtg cagcaaccgg aattcgccgc cgcgaaagat
     4921 ggctacacct tcgtatctca ccagcaggaa gtgggtacag gttacttcga taaagtgacg
     4981 actattattc agggcggcac gtcgtcggtt acagcgctga ccggctccac cgaagaatcg
     5041 cagttctaag caacaacaac cgttgctgac tgtaggccgg ataaggcgtt cacgccgcat
     5101 ccggcaatcg gtgcacgatg cctgatgcga cgcttgcgcg tcttatcatg cctacagccg
     5161 ttgccgaacg taggccggat aaggcattta cgccgcatcc ggcaatcggt gcacgatgcc
     5221 tgatgcgacg cttgcgcgtc ttatcatgcc tacaaccgtt gccgaacgta ggctggataa
     5281 ggcgtttacg ccgcatccgg taattctctg ctcctgatga gggcgctaaa tgccgcgtgg
     5341 cctggaatta ctgattgctc aaaccatttt gcaaggcttc gacgctcagt atggtcgatt
     5401 cctcgaagtg acttccgggg cgcagcagcg tttcgaacag gccgactggc atgctgtcca
     5461 gcaggcgatg aaaaaccgta tccatcttta cgatcatcac gttggtctgg tggtggaaca
     5521 gctgcgctgt atcactaacg gccaaagcac ggacgcggaa tttttactgc gcgtcaaaga
     5581 gcattacacc cggctgttgc cggattaccc gcgcttcgag attgcggaga gcttttttaa
     5641 ctccgtgtac tgtcggttat ttgaccaccg ctcgcttact cccgagcggc tttttatctt
     5701 tagctcccag ccagagcgcc gctttcgtac cattccgcgc ccactggcga aagacttcta
     5761 ccccgagcat ggctgggaag cgctgctgat gcgagtcctc agtgacctgc cgctgcgcct
     5821 gcgctggcag aataaaagcc gtgatatcaa ttatattatt cgccatctga aggaaacgtt
     5881 ggggccagaa aacctctccg aaagtcattt acaggtggcg aacgaactgt tttaccgcaa
     5941 taaagccgcc tggttggtag gcaaactgat cacgccttcc ggcacattgc catttttgct
     6001 gccgatccac cagacggacg acggcgagtt atttattgat acctgcctga cgacgaccgc
     6061 cgaagcgagc attgtttttg gctttgcgcg ttcttatttt atggtttacg cgccgctgcc
     6121 cgcagcgctg gtcgagtggc tacgggaaat actgccaggt aaaaccactg ctgaattgta
     6181 tatggctatc ggctgccaga agcatgccaa aaccgagagc taccgcgaat atctcgttta
     6241 tctacagggc tgtaatgagc agttcattga agcgccgggt attcgtggaa tggtgatgtt
     6301 ggtgtttacg ctaccgggct ttgatcgggt attcaaagtc atcaaagaca ggttcgcgcc
     6361 gcagaaagag atgtctgccg ctcacgttcg tgcctgctat caactggtga aagagcacga
     6421 tcgcgtgggc cgaatggcgg acacccagga gtttgaaaac tttgtgctag agaagcggca
     6481 tatttccccg gcattaatgg aattactgct ccaggaagca gcggaaaaaa tcaccgatct
     6541 tggcgaacaa attgtgattc gccatcttta tattgagcgg cggatggtgc cgctcaatat
     6601 ctggctggaa caagtggaag gtcagcagct gcgcgacgcc attgaagagt acggcaatgc
     6661 cattcgccag cttgccgctg ctaacatttt cccgggcgac atgctgttta aaaacttcgg
     6721 tgtcacccgt cacgggcgtg tggtttttta tgattacgat gaaatttgct acatgacgga
     6781 agtgaacttc cgcgacatcc cgccgccgcg ctacccggag gacgaacttg ccagcgaacc
     6841 gtggtacagc gtctcgccgg gcgatgtttt cccggaagag tttcgccatt ggctatgcgc
     6901 cgacccgcgt attggtccgc tgtttgaaga gatgcacgcc gacctgttcc gcgctgatta
     6961 ctggcgcgca ctacaaaacc gtattcgtga aggacatgtg gaagatgttt atgcgtatcg
     7021 gcgcaggcaa cgatttagcg tacggtatgg ggagatgctt ttttgagtaa agcttccata
     7081 taatttttct ccgcaatgta tcgagggtta tccgtaaagc caaagctttc agccatctta
     7141 tttaatgtat taaagattaa ttcagcaata acccggtgac ccaattcaca agccaactca
     7201 aaggcagagt atttttgtgg cgctttgtgt tgccaaaaat ccataatatc ttcagcggta
     7261 aatccaaaca ggcgtgcatg gtca
//
LOCUS       DAPGOF010000214         7253 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.215, whole genome shotgun sequence.
ACCESSION   DAPGOF010000214 DAPGOF010000000
VERSION     DAPGOF010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.215"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            139..381
                     /locus_tag="RHV13_004228"
     CDS             139..381
                     /locus_tag="RHV13_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243690.1"
                     /translation="MNSLAGIDMGRIILDLSNEVIKQLDDLEVQRNLPRTDLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(694..1623)
                     /gene="fdhE"
                     /locus_tag="RHV13_004229"
     CDS             complement(694..1623)
                     /gene="fdhE"
                     /locus_tag="RHV13_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HDV1243691.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKDPKIEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1620..2255)
                     /gene="fdoI"
                     /locus_tag="RHV13_004230"
     CDS             complement(1620..2255)
                     /gene="fdoI"
                     /locus_tag="RHV13_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HDV1243692.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2252..3154)
                     /gene="fdoH"
                     /locus_tag="RHV13_004231"
     CDS             complement(2252..3154)
                     /gene="fdoH"
                     /locus_tag="RHV13_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HDV1243693.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3167..6217)
                     /gene="fdnG"
                     /locus_tag="RHV13_004232"
     CDS             complement(3167..6217)
                     /gene="fdnG"
                     /locus_tag="RHV13_004232"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(5630..5632),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HDV1243694.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGYAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6411..7244
                     /gene="fdhD"
                     /locus_tag="RHV13_004233"
     CDS             6411..7244
                     /gene="fdhD"
                     /locus_tag="RHV13_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HDV1243695.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1622 a         1919 c         1953 g         1759 t
ORIGIN      
        1 ataataactg caactgttac atcatatctg gaaaacgcct cgcaaaaaca aaaagtgatg
       61 cgtaaatgcg ctttctttaa agattcgaca agggtacttt tctctccata gatatcaaat
      121 gatattggtc aaatatttat gaactctctg gcaggtattg atatgggcag aattatactc
      181 gatttatcga atgaggtgat taagcaactt gatgatcttg aggtgcagcg taatcttcct
      241 cgtacggacc tattaaggga agcggtagat caatacctga taaatcaatc gcaaacagca
      301 agaaccagtg ttcctggcat ctggcaaggg tgtgaggaag atggtgtcga atatcagcgt
      361 aagctgcgcg aggaatggta atgggaaaaa atgatgtcaa tcagattgct gacaacgtgc
      421 gcgttgttca tgccggatgc ggcgtgaacg ccttatccgg cctacaaagt cgtataaatt
      481 caatgtattg cagtttattg gtaggcctga taagcgcagc gcatcaggca atcttacgct
      541 tgtcatcagt ctcatgccca taaccttatt gatgtggtgg tgggtaaatt ggcggcatag
      601 cttgaggtgg taacgcggaa tacgagagat ggtgtcgtca caccctatga gctgtaatcc
      661 gggcgaccag cgccacccgg aaaacgtcag agattactcc ccttcacccg gaaacaggaa
      721 cgggttaatg gaactacggg cgtagccttc ttgctccatt cgtgcgtcca gtaccagaga
      781 ggcgaggtca tctgctacgg cttcgatttt tggatctttt tcctgataga gaattttcag
      841 gtaagtgccg cagtcatcgc agctttcggc tttaatcgcg gcctgttcgt catccagcga
      901 ccagtaatgt agtttgccgc tctgctcgca gttgctgcat tttacgcgca caacgtgcca
      961 ttcagtttca cacaggttgc agtgcaggta acgtagcccc tgagtggtgc caatttgcac
     1021 catgctggac accggcatag aaccacaaac cgggcaatat tgacgttgct cgccgtattc
     1081 agcgcgggct ttgccgggga tcagattggc catctgcgcc cagtagagcg acagtgcagc
     1141 ccagataaac ggcgctttat cgctgctgac ggacgagaaa tcagaggcaa acagtgcgct
     1201 ggccatatct tccagctcct gagtcgatgc cttctccaga ttctcaatca ctgccagcgc
     1261 cgggccgctc atttcaggtt tcagctcagc aattagcgcc atcagcagct tttgccagtg
     1321 cttatcacgc ggcagaacgt gaatatccag cgggggcttg ccttgtgcgc tggcttcttt
     1381 aatgcgcgca gtcagatcca tctccagcgg atggtcgtac agcaccactt cctgggcgtg
     1441 ggcgataagc gcagcaaagc gcaggtaatc acccagcgga ttattttctg ccagctcgcg
     1501 cagacgctcg gcgcggcggt tgtataaatt cttgagccga gggaacaata acggcggaat
     1561 catatccgcc gtacgtttct cgctcgaacc cagctcatct tgcgggatta tgcgaatact
     1621 cattcagctt tcttttccgt tgtcttgcgg acctcacggt accagcgcgg gtgatgtttc
     1681 ttcgcccagg cgctggtaac ccatccttcc accatggcgg taatcgtgcc tttcacccaa
     1741 agggcggcgt agatatgcac catgataacc acaattaacg ccactgcggc aaatgaatgc
     1801 agcattaacg cgaatcggat caccgggatt gagaaagcag gcgcaaaata aggacgccag
     1861 ataatcacgc cgctcaccag cagcagaacc aggaaaataa tcgccgccca gaaaacgcat
     1921 ttctgaccga agttataacg cccggtgtca cctacttcct cgttgacgac gatcttacga
     1981 atattcttcg cccaaaagat atcatcccga ttgattaggt tgtgatgcca gtaacggaaa
     2041 aacatgatga tgaacgaggc aaacataacc acgccgacaa acgggtgcag aattcgcgcc
     2101 agctgcggtg tgcccatgat ttgcatcaac cagttgaagg acgggaacaa aaagcccagc
     2161 ccgctcaccg ccgccaggat gaagcagaag gcggtgatcc agtggttgat acgttccggc
     2221 gcggtgtagc gcacgatggt gtcacgtcgt ttcatttgcg ctcctcgtct ttctcttcgt
     2281 gcagattatt ctcttcctca tccgcacggt tcggaccgac acccacgtag tggaagatac
     2341 tggctgcgaa ggtagccgca aagccaacag ctgcgagcgg tttccagatg cctttccaga
     2401 atttcacggt ttcgctgatt tccgggttct ccggcaagcc atgatacaga tttggcttgt
     2461 cagcatggtg cagcacgtac ataacgtgtg taccaccgac gcctgccgga tcgtacaggc
     2521 ccgcattgtc gtaaccgcgg gttttcagct ccgccacgcg ctcgctcgcc agcgttttca
     2581 tcgactcttt cgtaccgaag tgaatcgcgc ccgttgggca ggtcttcacg caggctggtt
     2641 cttgcccaac caccacgcgg tcaacgcaca gcgtacattt gtagacgcgg ttgtcttccg
     2701 ggttgaggcg cggaatgtcg aacggacagc ccgcaatgca ataaccgcag ccaatgcact
     2761 gctcggactg gaagtcgacg ataccgttgg catactgaat aattgcacct tccgccgggc
     2821 acgctttcag gcagcctgga tcggaacagt gcatacagcc gtctttgcgg atcagccatt
     2881 ccagtttgtc gttctgctcc acttccgaga agcgcatcac cgtccacgat ttggcactta
     2941 aatcattggg gttgtcgtac accccaatgt tattgccgac ggtatcgcga atgtcgttcc
     3001 actctgaaca cgccacctga caggctttac agccgataca ggtggtgacg tcgatgagtt
     3061 tcgccacttc ttcctggaag tcccgcgcct gaggcgcggg ggtcagaccg ttagtcgcgg
     3121 aacgacgaat gatatcttgc gattgataag ccatatgtcg tctccgttac accttttcca
     3181 cattcacaag gaaggactta aactccggcg tctgcgtgtt cgcatcacca acgaatggcg
     3241 tcaacgtatt ggcaataaag ccttttttcg caacaccttc ataaccccag tgaatcggaa
     3301 taccgatggt atcgatatct ttgccgttcg ctttcagcgt gcgaatacgt ttggtcacca
     3361 ccgctttggc tttgatatag ccacggttgg aggagacttt cacagtatcg ccctgggcaa
     3421 tgccaagttt attcgccagc gactccccga tttccacaaa ctgctctggt tgcaaaatcg
     3481 cgttcaatag cgcgtgtttg gtccagtagt ggaagtgctc ggtcagacga taggtggttc
     3541 cgacatacgg gaacttatcg gctttaccca atgcttcggc gtcgtcttta aagatacgtg
     3601 cagccgggtt cgagataacg tttggatgca gcgggttagt tcccagcggc gtttcaaacg
     3661 gctcgtagtg ttccgggaac ggaccttccg ccatcttatc gagggcaaac agacgcccca
     3721 tgccttcctg ctgcatgata aacggcccga cgccgctacc cggaggcgct gcgctgtagt
     3781 ccggaatatc ccagccggtc cacttagtgc cgtcccattt cagcaactga cgcttcggat
     3841 cccacgggtt accctgcgga tctgcggagg cgcggttata cagaatgcgg cggttaagcg
     3901 gccatgccca tgcccagccc agcgtgttac cgaggccaga cggatcggcg ttatcacgac
     3961 gcgccatctg gttgccttcc ggcgtccagc taccggcgaa aatccagcag ccacaggagg
     4021 ttgtaccgtc atcgcgcagt tgggcgaacg agctaagttg ttggcctttc ttgacgataa
     4081 ccgcaccggt tgccgggtcg gtaatatcgg ccagcgcctt accgttgctc tccatcgcca
     4141 cttcttccga cttcggctca tgcggaatgg cgtagttcca ggtcatgttc agcacctgat
     4201 ccgggtttgc gccaccctgt tcggcataca tcttgcgcaa gcgcaggaag ataccggaga
     4261 ggatctcgcc gtcagtcagc gcaatccccg gggcgtccgc acctttccag tgccactgta
     4321 accagcgacc tgagttaacg attgaaccgt tctcttctgc gaagcaggtc gatggcagac
     4381 ggaacacttc ggtctggatc ttcgacgagt caacttcgtt cagttcaccg tggttctgcc
     4441 agaagttaga ggtttcagtg ttcagcgggt cgatggtgac gaggaacttc agtttcgaca
     4501 gacaaccgat cactttgttt ttgttcggga atgaggcaac agggttaaag ccctggcaga
     4561 tatagccatt gaccttgccc tctttcatca tctcgaagta ttgcaggacg tcgtagcctt
     4621 tatcccactt cggcaaccag tcaaagcccc agctattttc cgccgtcgct ttatcaccaa
     4681 agaaggcctt catcatagag acgaagaatt tcgggtagtt gccccagtag tttacctggc
     4741 cttccagcag cggttttggc gtgttggcgg taaggtaggt ttgcagatcg gtctgcttct
     4801 cgcttggcag cgtcatgtaa cctggcaggc tctgcgacag aaggcccagg tccgtcagcc
     4861 cctgaatatt ggagtgaccg cgcagggcgt taacgccgcc gcctgccatc cccatgttgc
     4921 cgagcagcag ctggatcatc gccatcgtac gaatgttttg cgcaccaacg gagtgttgcg
     4981 tccagccgag ggcatacagg aacgaggcag ttttatcgtg agcactggtt tctgcgatgt
     5041 attcgcagac tttcaggaac gcgtcttttg gcgtaccaca gatgttttca accacatctg
     5101 gcgtgtagcg ggaaacgtgc tgtttcagca agttccacac gcagcgcgga tgttgcagcg
     5161 tggtatcgcg tttggcgaag ccgttttcgt ccagttcata agtccaggag gatttatcgt
     5221 acttgcgttt ttccgcgtcg tagccggtga acaggccatc ttcaaagccg taatcctcac
     5281 gcacgatcag gctggcgttg gtataggctt cagtgtattc gcggttgaat ttttcattgt
     5341 tcagcaggta cagcaatacg cctgacagga aagcaatgtc agtaccggaa cgaatagggg
     5401 catagtagtc agccaccgca gccgtacgcg taaagcgagg atcgatcaca atcagcttcg
     5461 cgccattgtg aattttggct tccatcgccc agcggaaccc gaccgggtga gcttcagcgg
     5521 cgttaccgcc catcaccacg acgaggttgg cgttcttgat gtcgacccag tggttggtca
     5581 tcgcaccgcg accaaatgtt ggagcaagac ttgctaccgt tggtccgtgt cagacacgcg
     5641 cctggttgtc gaccgcgagc atacccagcg cgcgggagaa tttttgcgtt aaatagccgg
     5701 tttcgttact cgacgcggaa gcacacagca tcccggtgga gagccagcgg ttaacagtca
     5761 cgccttcggc gttttgcgca atgtagttag catcgcggtc ttctttcatc agtttggcga
     5821 tgcgatcaaa cgcctcttcc caactgattt gctgccattt atcagaacct ggcgcacggt
     5881 attccggaaa cttcagacgg ctttcggagt ggatgaaatc caccaggcca gcgcctttcg
     5941 gacaaagtgc accgcggttg accgggtgat ccggatcgcc ttcgatatgg aagatagatg
     6001 ctttggcgtt ttttgctccg tcaccgaggc tgtacatcaa cagcccacag cctacggaac
     6061 aataggtgca ggtattacgg gtttcgcggg tgcgcagcag tttatactgc cgggtttccg
     6121 cgagtgctac gctgggtgca taacccagtg ccgctgccgt ggtgcctgcc ataccgccag
     6181 cgcagatctt aaagaactgc cttctgctga cctgcatgga ttgctccttg tttcgacatt
     6241 gtcacgtccc atttacattc gcttgctgcg tgtgcaggga gtgggagtta tttttctttg
     6301 cggaaggggc cgcaaaggtc cagaattggc tcaatttccc tccatccagg aagggtttgt
     6361 aacagaatac cataatgttg gtgtgtgtgt tcttatctgg ttaagagaaa gtgaaaaaaa
     6421 cacagcaaaa agaaatcgaa aatgtgacaa atatcacagg tgttcgtcaa attgagttat
     6481 ggcggcgtga tgatttacaa cacccacggt tagatgaggt cgcggaagaa gttcccgttg
     6541 cgctggtcta caacggcatt tcgcatgtgg tgatgatggc gtcgcccaaa gaccttgagt
     6601 actttgcgct cggtttttcg ctttccgagg ggataataga aagtccgcgc gacatcttcg
     6661 gcatggatgt cgttccttcc tgtaatggtc ttgaagtaca aattgagctt tccagccgcc
     6721 gctttatggg gttgaaggag cgccgccggg cgctggcggg acgtacgggc tgtggcgtat
     6781 gcggcgtgga gcaacttaat gacatcggaa aaccggtgca gccgctaccg ttcacccaga
     6841 cgtttgatct caacaaactg gatgatgcat tacgtcatct caacgatttt cagccagtgg
     6901 ggcaactgac tggttgtact cacgccgctg cctggatgtt gccatctggc gaactggtcg
     6961 gcgggcatga agacgtgggt cgccatgtgg cgctggacaa actgttaggc cgccggtcac
     7021 aagaagggga aagctggcag caaggtgcgg tactggtttc cagtcgtgcc agttatgaaa
     7081 tggtgcaaaa gtcggcgatg tgcggcgtag agattttgtt tgcggtgtct gccgcgacca
     7141 cgcttgctgt agaagtggct gagcgctgta atttgacact ggtaggtttt tgtaaaccgg
     7201 gtcgggcaac ggtttatacc catccgcagc gtttgagcaa ttaattaaaa taa
//
LOCUS       DAPGOF010000215         7187 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.216, whole genome shotgun sequence.
ACCESSION   DAPGOF010000215 DAPGOF010000000
VERSION     DAPGOF010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7187
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.216"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(45..356)
                     /locus_tag="RHV13_004234"
     CDS             complement(45..356)
                     /locus_tag="RHV13_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDV1243696.1"
                     /translation="MKKILLVCAAGMSTSMLVKRMIDHANAISLEVNISALAIAEAKG
                     KIKNNEVDVVLLGPQVRFQKPEIEAVAQGKMPVSVIEMKDYGTMNGQAVLEFAMKLLQ
                     E"
     gene            555..1253
                     /locus_tag="RHV13_004235"
     CDS             555..1253
                     /locus_tag="RHV13_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDV1243697.1"
                     /translation="MIFQKIARLLKSEINGYSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGTYIQKKNFVAHIDHMNSFSEIAHKSGKEAGSQIMKFEVQD
                     ASPTIATELNLVTGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVAHMQKSKFSY
                     IENECGIKIIGTFETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSVPLDYSLLY
                     SNIFEFQVKYFFPR"
     gene            complement(1297..2196)
                     /gene="eamA"
                     /locus_tag="RHV13_004236"
     CDS             complement(1297..2196)
                     /gene="eamA"
                     /locus_tag="RHV13_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HDV1243698.1"
                     /translation="MSRKDGVLAILVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHATRPAVMSLVIWSALIPIIPFFVASLILDGSATMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFFGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            2391..3578
                     /gene="ydeE"
                     /locus_tag="RHV13_004237"
     CDS             2391..3578
                     /gene="ydeE"
                     /locus_tag="RHV13_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HDV1243699.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAVNPLVSGVVLTTLPSSSLFIILALVIIAAWALMLKGINARPQRQP
                     ALC"
     gene            complement(3619..4014)
                     /locus_tag="RHV13_004238"
     CDS             complement(3619..4014)
                     /locus_tag="RHV13_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDV1243700.1"
                     /translation="MRYVTEFTTAKPEVAEAHFLARLSVETDCADVHDSLAYIGDDFV
                     LLHVVGREEDFARRHIPGALHLPHKQMTAERMAEWPDDTLFVVYCAGPHCNGADRAAL
                     KLARLGKPVKIMLGGITGWEDEGYAFASE"
     gene            4109..5080
                     /gene="ftrA"
                     /locus_tag="RHV13_004239"
     CDS             4109..5080
                     /gene="ftrA"
                     /locus_tag="RHV13_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FtrA"
                     /protein_id="HDV1243701.1"
                     /translation="MTTLQRPLVIALAYDGLCTFEFGVAVEIFGLPRPELGDNWYRFA
                     VAAVEPGALQATGGIHVVADGGLELLPNADMIIIPGWRGIDAKVPEMLCQSLREAHQR
                     GTRLVSICSGVFVLAAAGLLHQRKATTHWRYTEQLQHRYPSISVSDNVLYQDEGQVMT
                     SAGSAAGIDLCLHIVRKDFGHEIANNVARRLVIQPHRQGDQPQNLTRPMASPRESQTL
                     GALFDFLQQNLAQTHTVSSLAERVNMSPRTFLRRFEALTGMTPARWLLNERLIRVQDY
                     LQNSVFNIEKIAELTGFGNSALLRHHFHQRFLLSPSQYRKNSRSPSQ"
     gene            5188..5283
                     /gene="mgtS"
                     /locus_tag="RHV13_004240"
     CDS             5188..5283
                     /gene="mgtS"
                     /locus_tag="RHV13_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HDV1243702.1"
                     /translation="MLGNMNVFMAILGIILFSGFLAAYFSHKWDD"
     gene            complement(5502..6392)
                     /gene="dgcZ"
                     /locus_tag="RHV13_004241"
     CDS             complement(5502..6392)
                     /gene="dgcZ"
                     /locus_tag="RHV13_004241"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HDV1243703.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FAAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAISHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(6647..7039)
                     /gene="ydeI"
                     /locus_tag="RHV13_004242"
     CDS             complement(6647..7039)
                     /gene="ydeI"
                     /locus_tag="RHV13_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HDV1243704.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKTMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
BASE COUNT         1932 a         1746 c         1540 g         1969 t
ORIGIN      
        1 gtgtgaaaag ttagttcagg taatagttta ataatattag attcttactc ttgcagtagt
       61 ttcatcgcaa attcaagaac tgcctgtccg ttcatggtgc catagtcttt catctcgata
      121 acggatacag gcattttccc ctgtgcaacg gcttcaatct ccggtttctg aaagcgaact
      181 tgcgggccaa gtaaaacaac gtcaacttcg ttatttttaa ttttcccttt agcctctgca
      241 atcgccaatg cggaaatatt aacttcaagt gaaatagcgt tagcatgatc aatcatacgt
      301 ttaaccagca tactggttga catgcccgca gcgcaaacta acaatatctt tttcatattt
      361 tcccttactg gtatataaca gactacattg tgtaaatatc atatcccgca gtagctatac
      421 atcctggtac attgatcaca gtaatacagt aataaagttg ttcaacatca gggtgttaat
      481 taaacgactc ggccatacga taaaccaatc aggactcaac cccttgtttt caatggctct
      541 taaaaggtgc aaaagtgatt tttcaaaaga ttgcccgctt acttaaatcc gagatcaatg
      601 gctattcatg gcatgttggt gatttgttgc catcagaagc tgaactcgca gttcgttata
      661 atgtttcgcg taataccctg cgtaaggcat tatccttgct ggaaggcgaa ggaattattc
      721 acagaaagca tggttcagga acatacattc aaaaaaagaa ttttgttgca cacattgatc
      781 acatgaacag tttcagtgaa attgcgcata aaagcggcaa agaggcaggg agtcagatta
      841 tgaaatttga agtgcaggat gcttcaccta ctattgcgac tgagctgaat ttagtaactg
      901 gtgagcaggt ttattacata aagagactgc gattcattga ggataatgca gcacaattag
      961 aagaaacgtg gatgtcagtg gcacgttttc ctgatttaac cgtagcacat atgcaaaaat
     1021 ccaaattttc gtatattgag aacgaatgcg ggatcaaaat cattggcacc tttgaaactt
     1081 tctcccctac ttttcctacc ccagaaatcg ccagtatttt acggatcagc ccacgggatc
     1141 ccatacttaa aattcagacc caggctgtgg acagtaactc tgttccgctg gattattcgt
     1201 tactttacag caatattttc gagttccagg taaagtactt tttcccgcga taaagcagac
     1261 gcacagagtg ctgacatcac attctgtgcg tatggtttag cctctaacct ttaccgcttt
     1321 acgccaccgc aagccaaata cattgatata cagcccggtc ataatgagca ccgcaccgaa
     1381 aaattgcaga cccgttaagc gttcatccaa caatagtgcc gcacttgcca gtccgactac
     1441 aggcaccagt aacgataacg gtgcaacccg ccaggtttca tagcgtccaa gtagtgttcc
     1501 ccagatccca taaccaacaa ttgtcgccac aaaagccaga tacatcagag acaagatagt
     1561 ggtcatatcg atagtgacca gactgtgaat catggttgcg gaaccatcga gaatcagcga
     1621 ggcaacaaag aagggaatga ttgggattaa tgcgctccag attaccagcg acatcaccgc
     1681 cggacgcgtt gcgtgcgaca tgatcttttt attgaagatg ttgccacacg cccaactaaa
     1741 tgctgccgcc agggtcaaca taaagccgag catcgccaca tgctgaccgt tcagactatc
     1801 ttcgattaac accagtacgc caaaaatcgc taaggcgatc cccgccaatt gtttgccatg
     1861 cagtcgctcc ccgaaagtaa acgcgccaag cacgatagta aaaaacgcct gtgcctgtaa
     1921 caccagcgaa gccagtccag caggcatacc aaaattaatg gcgcagaaca gaaaggcaaa
     1981 ctgcgcaaaa ctgatggtta atccataccc cagcagcaaa ttcagtggta ctttcggtcg
     2041 tgcgacaaaa aagatggccg gaaaagcgac cagcataaag cgcaaaccgg ccagcatcag
     2101 cggtggcatg ttatgaagcc ccactttgat gaccacaaaa tttagccccc atacgaccac
     2161 taccagtatc gccaacaccc catcttttcg cgacattcta ccgcctctga atttcatctt
     2221 ttgtaagcaa tcaacttagc cgaatttact tttctttaac agttgattcg ttagtcgccg
     2281 gttacgacgg cattaatgcg caaataagtc gctatacttc ggatttttgc catgctattt
     2341 ctttacatct ctaaaacaat acataacgaa acgcactgcc ggacagacaa atgaacttat
     2401 ccctacgacg ctctaccagc gcccttcttg cctcgtcgtt gttattaacc atcggacgcg
     2461 gcgctacgct gccatttatg accatttact tgagtcgcca gtacagcctg agtgtcgatc
     2521 taatcggtta tgcgatgaca attgcgctca ctattggcgt cgtttttagc ctcggttttg
     2581 gtatcctggc ggataagttc gacaagaaac gctatatgtt actggcaatt accgccttcg
     2641 ccagcggttt tattgccatt cctttagtga ataacgtgac gctggtggtg ctcttttttg
     2701 ccctcattaa ctgcgcctat tccgtttttg ctaccgtgtt gaaagcctgg tttgccgaca
     2761 atctttcgtc caccagcaaa acgaaaatct tctcaatcaa ctacaccatg ctcaacattg
     2821 gctggaccat cggtccgccg ctcggcacgc tgttggtgat gcagagcatc aatctgccct
     2881 tttggctggc agctatctgt tccgcgtttc ccatgctttt cattcaaatt tgggtaaagc
     2941 gcagcgagaa aatcatcgcc acggaaacag gcagtgtctg gtcgccgaaa gttttattac
     3001 aagataaagc actgttgtgg tttacctgct ctggctttct ggcttctttt gttagtggcg
     3061 catttgcttc atgcatctca caatatgtga tggtgattgc tgatggggat tttgccgaaa
     3121 aggtggtcgc ggttgttctt ccggtgaatg ctgccatggt ggttacgttg caatattccg
     3181 tgggccgccg acttaacccg gctaacatcc gcgcgctgat gacagcaggc accctctgtt
     3241 tcgtcatcgg tctggtcggt tttatttttt ccggcaacag cctgctattg tggggtatgt
     3301 cagctgcggt atttactgtc ggtgaaatca tttatgcgcc gggcgagtat atgttgattg
     3361 accatattgc gccgccagga atgaaagcca gctatttttc cgcccagtca ttaggctggt
     3421 tgggcgcagc ggtaaatcca ttagtgagtg gtgttgtact taccactctc ccgtcgtctt
     3481 cgctgtttat cattctggcg ttagtcatca ttgccgcgtg ggcgctgatg ttaaaaggta
     3541 ttaacgccag accccagagg caaccagcac tctgttgacg ttcgtgccgt tccactactg
     3601 gcacggcttc gctaatcatt attctgaggc aaaggcatag ccttcatctt cccagccggt
     3661 tatcccgccg agcatgattt taaccggctt tccgagtcga gccagtttga gtgccgcacg
     3721 gtctgcaccg ttacaatgtg gccccgcgca atacaccaca aatagcgtat cgtcgggcca
     3781 ttcggccatt ctttctgcgg tcatttgctt atggggtaaa tggagagcgc ctgggatatg
     3841 ccgacgcgcg aagtcctctt cacgtccaac cacatgaagt aaaacgaaat catcgccaat
     3901 ataagccaga gagtcatgaa catcggcgca atctgtttcg acactcaagc gcgcgagaaa
     3961 atgcgcctca gcaacttctg gcttcgctgt ggtaaattca gtgacataac gcatattgct
     4021 ttgtcctttt ggtttgttgt gttaacaata atgtaactaa tctacctttc actgaccgcc
     4081 tctcgatatg ccagaaatga gcaaaatgat gacaacgtta caacgtccgc tggtcattgc
     4141 actggcatat gacgggctat gtacttttga atttggcgtt gccgttgaaa tattcggcct
     4201 gccacgaccg gaactgggcg ataactggta tcgttttgct gttgctgcgg tagaacctgg
     4261 tgcgctacaa gccaccgggg ggatccacgt ggttgcagat ggtggactgg agttattgcc
     4321 caacgccgat atgattatta ttcctggctg gcgcggaata gatgccaaag tgccggagat
     4381 gctatgccag tcattgcgcg aggcacacca gcgcggtact cgcctggtat caatatgctc
     4441 tggcgtgttt gtccttgctg ctgcgggcct tcttcatcag cgaaaagcaa ccacccactg
     4501 gcgctacact gaacaactcc agcaccgtta cccttcaatc agcgtaagtg ataatgtgtt
     4561 atatcaggat gaaggacaag tgatgacctc cgccggaagc gccgcgggga tcgatctgtg
     4621 tttacatatc gtgcgcaaag attttggtca tgagattgct aataatgtcg cccggcgact
     4681 ggtcattcag ccacatcgcc agggggatca accacaaaat ctgacgcgcc caatggcctc
     4741 accgcgtgaa agccagacgt taggtgcctt attcgacttt ttgcagcaga atctggcaca
     4801 aacacatact gtgtcttccc tcgccgaacg ggtaaatatg agtccgcgca cttttttaag
     4861 acgttttgag gcgttaactg gcatgacgcc cgcccgctgg ctgctcaatg agcgcttaat
     4921 acgagtacag gattatctgc aaaattcggt ttttaacatc gagaaaattg ccgagctgac
     4981 gggttttggc aattcagcgt tattgcgtca tcactttcac caacgttttt tattatctcc
     5041 ttcgcaatat cgcaaaaaca gccgttcccc atcacaataa tcgcctgatt cagcctttgt
     5101 ttagataacc tctgctaata tctttctcat tgagataaaa attaaggtaa gcgaggaaac
     5161 acaccacacc ataaacggag gcaaataatg ctgggtaata tgaatgtttt tatggccata
     5221 ctgggaataa ttttattttc tggttttctg gccgcgtatt tcagccacaa atgggatgac
     5281 taatgaacgg agataatccc tcacctaacc ggccccttgt tacagttgtg tacaaggggc
     5341 ctgattttta tgacggcgaa aaaaaaccgc cagtaaaccg gcggtgaatg cttgcatgga
     5401 tagatttgtg ttttgctttt acgctaacag gcattttcct gcactgataa cggatcgttg
     5461 acacagtagc atcagttttc tcaatgaatg ttaaacggag cttaaactcg gttaatcaca
     5521 ttttgttcgt caataaacat gcagcgattt cttccggttt gcttaccctc atacattgcc
     5581 cggtccgctc ttccaatgac cacatccaga ggctcttcag gaaatgcgcg actcacacct
     5641 gctgtcacgg taatgttgat atgcccttca gaatgtgaga tggcatggtt atcgactaac
     5701 tggcaaattc tgacacctgc acgacatgct tcttcatcat tagccgcttt gacaataatg
     5761 ataaattctt cgcccccgta gcgataaacc gtttcgtaat cacgcgtcca actggctaag
     5821 taagttgcca gggtgcgtaa tactacatcg ccgattaaat gcccgtaggt atcattaacc
     5881 aatttaaatc ggtcaatatc caacaacatt aaataaagat tcagaggctc agtgttgcgt
     5941 aactgatgat caaaggattc atcaagaacc cgacgccccg gcaatcccgt caaaacatcc
     6001 atattgctac ggatcgtcag caaataaatt ttgtaatcgg ttaatgccgc agtaaaagaa
     6061 agtaaccctt cctgaaaagc ggcgaaatgc gcgtcctgcc agtgattttc aacaatagcc
     6121 agcattaatt cccgaccaca gttatgcata tgttgatggg cagaatccat tagccgaacg
     6181 taaggtaatt catcgttatc gagtggcccc agatgatcaa tccaccgacc aaactggcac
     6241 agtccataag aatggttatc cgttatttct ggcttactgg catctctcgc gaccacgctg
     6301 tgaaacatac tcaccagcca ctggtagtgg gcatcgatag ccttattgag atttaacaag
     6361 atggcatcaa tttccgttgt cttcttgatc attgccactc ctttttcaca gttccttgtg
     6421 cgcgttattc taacgagaga aaagcaaaat tacgtcaata ttttcataga aatccgaagt
     6481 tatgagtcat atctgagata acattgtgat ttaaaacaaa atcagcggat aaaaaagtgt
     6541 ttaattctgt aaattacctc tgcattatcg taaataaaag gatgacaaaa agcataaccc
     6601 aataccctaa tggcccagta gttcaggcca tcaggctaat ttatttttat ttctgcaaat
     6661 gagtgacccg aacgacggcc ggcgcgcttt tcttatccag actgccgcta atgttgatca
     6721 tctggtccgg ctgaacttct cgtccatcaa aaacggccgc aggaataaca acattaattt
     6781 caccgctctt atcgcgaaaa acgtaacggt cctctccttt gtgagaaatc aaattaccgc
     6841 gtagtgaaac cgaagcgcca tcgtgcatgg tttttgcgaa atcaacggtc attttttttg
     6901 catcatcggt tccgcgatag ccatcttcta ttgcatgagg cggcggtggc gctgcatcct
     6961 gttttaaacc gccctggtca tctgccaacg cataaggcat gacaagaaaa cttgctaata
     7021 caatggcctg aaatttcata ctaactcctt aattgcgttt ggtttgactt attaagtctg
     7081 gttgctattt ttataattgc caaataagaa tactgccaat tgttataagg catttaaaat
     7141 cagccaacta gctgtcaaat attcagagaa tttaactcac taaagtt
//
LOCUS       DAPGOF010000216         7171 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.217, whole genome shotgun sequence.
ACCESSION   DAPGOF010000216 DAPGOF010000000
VERSION     DAPGOF010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7171
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.217"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(322..1458)
                     /locus_tag="RHV13_004243"
     CDS             complement(322..1458)
                     /locus_tag="RHV13_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDV1243705.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1461..1823)
                     /locus_tag="RHV13_004244"
     CDS             complement(1461..1823)
                     /locus_tag="RHV13_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HDV1243706.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKVTDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2360..4273
                     /gene="mtlA"
                     /locus_tag="RHV13_004245"
     CDS             2360..4273
                     /gene="mtlA"
                     /locus_tag="RHV13_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HDV1243707.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEEKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4503..5651
                     /gene="mtlD"
                     /locus_tag="RHV13_004246"
     CDS             4503..5651
                     /gene="mtlD"
                     /locus_tag="RHV13_004246"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDV1243708.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYGLPYKNLIQGIAAAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            5651..6238
                     /gene="mtlR"
                     /locus_tag="RHV13_004247"
     CDS             5651..6238
                     /gene="mtlR"
                     /locus_tag="RHV13_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HDV1243709.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(6249..6458)
                     /locus_tag="RHV13_004248"
     CDS             complement(6249..6458)
                     /locus_tag="RHV13_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243710.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQAEEK"
     gene            6743..7105
                     /locus_tag="RHV13_004249"
     CDS             6743..7105
                     /locus_tag="RHV13_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HDV1243711.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTAF"
BASE COUNT         1817 a         1795 c         1817 g         1742 t
ORIGIN      
        1 ctatagatta aatatttttt aaatgtgact gtcatcattc agataataaa gccatcatcg
       61 caatagatgg aaaagcactc ctgctctttt ttgattagag ccgtcgcagg aggaacaact
      121 catatcatca ttgcgatctc gacaatgtat agtctgatcc ttcggcagat caagacggat
      181 aaaaaatcca atgagttcac actcatcccg aacttcttaa catagtggat attaacgaaa
      241 aatcatcaca gccgatgaaa taatatgcaa aaataattag tccttacaag gagcaaagtt
      301 ccataatctc ccccctcccc ctcaatgatc caaataaaga taatgcatcc agctggtcat
      361 tcttagcagc actttccgca tcaccgaaac atggctgaaa tggtcggagt aaactgccac
      421 ctgggcgtaa atgccgtcgg gtaaggcatc gatatcatcg ttagggtcca gttcaatggt
      481 tcccagcaca ccgtccgtgc cgggcacgac cgttaatgat tgcaataccc cctgcgcctg
      541 ataagaaccg cctggcacga caggtaaaat actggtcagt ttgccgtgga acacctgccc
      601 aggtagcgcg ttaaacacca cttccgcatc atcaccaggt ttcagacgta acagcgagtt
      661 ttgccgaaat tgggcgacaa tttgccgttt ttgctcgggg atgaaaacca tcaccggacg
      721 caacggcaag gcagctgcgt atgtgcctgg gcggatcagt acctgagtga cgtagccatt
      781 gcttggtgcg cgaatgacag tctgctcaag attatatttt gcttcagtaa gttgcgctct
      841 taagctcaca atctgcgatt gctcgccgtt gaccatactg tcgagctgac tctggatctg
      901 cgcctgctcc gccaccgagc ctttcactaa cgcatcctgc gcgaggaaat tttgccgcgc
      961 atcgtcgatg tcacgttccg agaacggatt caccgccgcc tggctgcctt tcaggtaacg
     1021 ttgataattt ttaaacagac ggtcgcgctc cgctgaaacc tgggtggtgt tggcctgcgc
     1081 ttcagtgagc tgcgcgcgca gcgtctttat attatgcgtc gccgtcatca ggtcagcctg
     1141 aagtctgtca actcgcgcct ggtaacgaac cgggtcgagc ttaaaaagca cctcgccctt
     1201 ttgaataagc tgattattct tgtcagtgac ttcagtaaca attcccgtca cctgtggcgt
     1261 gatagggatc gctatcactg ccttttgcgc ggtaaaagtg taagggtggt tgtagttcat
     1321 caacaaaatc aaaccactca ccagaaacac gcctcccagc gccgccgtcg ccagcgtcca
     1381 ctgattcacc ggaatgcgga agattttaaa gaccgcccac gccagcgcca cgtaagttaa
     1441 aacaatcaat agatccataa ttagatctcc gggaacgtgg aataatcagt ctttttctcg
     1501 gcggcgagtt ggtgctccag cctggaaatt cggtcagaaa gtgcggcgat ttccgggtca
     1561 gtcactttct cctgcgacgc aacatgtgac tgcatgcccc agccgcgctc cggttgatag
     1621 agcgtcgccc agatccacag aaacggccag ataacatgca gagtaaacag gctcacccag
     1681 cccgccgtat gaatagcgtc ggcatgggga tggttgcgct ttttggcaat caaataaggg
     1741 atgtcatgta tcgcgatgat tccataaaaa atcaccagaa agacgaagat cagcactccc
     1801 aacgcgaaat agtttagaaa catatctgcc tcggattcac gtttatcagt gttgtttttt
     1861 ggctggcagc cagaagggag tcaggctgat attttgacaa taatccgggt tcgcgattct
     1921 cgccataaca ccaaagaata atttttagag gtgatgagtt gcttagttac ataacgattg
     1981 tatgacgaag gcataacatg ctgtagatca catcaggtga acgccgtaag aaaatatctt
     2041 gtgattcaga tcacaaagat tcaacaaacc atcaaaacaa aaatgtgaca ctactcacat
     2101 ttaaatgcca tttttagcga aaatcgccgc cttgttgctt ttttacacaa gtgttttgtg
     2161 atgaacgtca cgtcaattac ctctctaccc cctatattta tgtgattgat atcacataaa
     2221 aggccgtcga ctggacagtt aaccgattca gtgccagatt tcgcagtatc tacaaggtcc
     2281 ggctacctct gccgccacat taacaaaaaa cctcgggctt caagcctgcg cgacagcaaa
     2341 cataagaagg ggtgttttta tgtcatccga tattaagatc aaagtgcaaa gctttggtcg
     2401 tttcctcagc aacatggtga tgccaaatat cggcgcgttt atcgcgtggg gtatcatcac
     2461 cgcgttattt attccaacag ggtggttacc gaacgagacg ctggcgaagc tggtcgggcc
     2521 gatgatcact tatctcctgc cgctgctgat cggttatacc ggtggtaagc tggtaggcgg
     2581 cgaacgtggc ggcgtagtcg gtgccatcac caccatgggc gttatcgtcg gcgcagacat
     2641 gccgatgttc ctcggttcta tgattgcagg tccgctgggc ggctggtgca ttaagcactt
     2701 cgaccgctgg gtagacggta agatcaaatc cggttttgag atgctggtga ataacttctc
     2761 cgcaggcatc atcgggatga tcctcgccat tctggcattt ctcggcattg gcccgattgt
     2821 tgaagccctg tccaaaatgc tggctgcggg cgttaacttc atggttgtcc atgacatgct
     2881 gccgctggcg tctatctttg ttgaaccggc gaaaatcctg ttcctcaaca acgccattaa
     2941 ccacggtatc ttctcgccgc tgggtattca gcagtcccat gaactgggta aatccatatt
     3001 cttcctgatt gaagctaacc caggtccggg tatgggcgtg ctgctggcgt acatgttctt
     3061 tggtcgtggt agcgctaaac agtctgcggg cggtgcggca attatccact tcctgggtgg
     3121 tatccacgaa atttacttcc cgtatgtgtt gatgaatccg cgtctgatac tggccgttat
     3181 cctcggcggt atgactggcg tgttcacgct gactatcctg ggcggtggtc tggtttctcc
     3241 ggcatctccg ggttctatcc ttgctgtact ggcgatgaca ccaaaaggtg cttatttcgc
     3301 taacatcgcg ggtgtgtgtg cggcgatggc tgtctccttt gttgtctctg ctattttgct
     3361 gaaaaccagc aaagtgaaag aagaagatga tattgaagca gcaactcgtc gtatgcagga
     3421 catgaaagct gagtctaaag gtgcatctcc gctgtctgcg ggcgatgtga ctaacgacct
     3481 gagccacgta cgtaaaatca tcgttgcctg tgacgccggt atgggttcaa gtgcgatggg
     3541 cgcaggcgtg ctgcgtaaga aaattcagga tgcaggtctg tcgcagattt cggtcactaa
     3601 cagcgcgatc aacaacctgc cgccagatgt ggacctcgtc atcactcacc gtgacctgac
     3661 cgaacgcgct atgcgccagg ttccgcaggc acagcatatt tcgctgacca acttcctcga
     3721 cagcggcctg tacaccagcc tgaccgaacg tctcgttgct gcccagcgcc atactgaaaa
     3781 cgaagagaaa gtaaaagaca gcctgaaaga cagctttgac gattccagtg ctaacctgtt
     3841 caagctaggc gcggagaaca tcttcctcgg tcgcaaagcg gcaaccaaag aagaagcgat
     3901 tcgttttgct ggcgagcagc tggtgaaagg cggttacgtt gagccggaat acgttcaggc
     3961 gatgctggat cgtgaaaaac tgaccccgac ttatctgggt gagtctatcg cggtgccaca
     4021 cggtacggtt gaagcgaaag atcgcgtact gaaaacgggc gtcgtgttct gccagtaccc
     4081 ggaaggcgtg cgcttcggtg aagaagaaga tgacattgcc cgtctggtga ttggtattgc
     4141 tgcccgtaac aacgagcaca ttcaggttat caccagcctg accaatgcac tggatgatga
     4201 gtccgtcatc gagcgtctgg cacacaccac cagcgtggat gaagtgctgg aactgctggc
     4261 aggtcgtaag taatccaatc ccaccctctt cacatggaga aggtggggtt aattgcctga
     4321 tgcgctacgc ttatcaggcc tacaggatgc atcacgattt gttgaatttg cacgttctgg
     4381 taggccggat aaggcgctta cgccgcatcc ggcgctaccc ctctcctcac ggagagggtt
     4441 tgggtgaggg aaaagcctca ccccagccct ctcgggtaaa aacattgatg aaggttaata
     4501 ctatgaaagc attacatttt ggcgcaggta atatcggtcg tggctttatc ggtaaactgc
     4561 tggcagacgc gggtatccaa ctgacgtttg ccgatgtcaa tcaggtggta cttgatgccc
     4621 tgaatgcccg tcatagctat caggttcatg tggttggtga aaccgagcag gtggataccg
     4681 tttccggcgt caatgctgtt agcagcattg gtgatgatgt cgttgatctc attgctcagg
     4741 ttgatttagt cactactgcc gttggcccgg tagtgctgga acgtattgct ccggctatcg
     4801 ccaaagggct ggtgaaacgt aaagaacaag gtaatgaatc tccgctgaac atcatcgctt
     4861 gtgaaaacat ggtccgcggc accacgcaac tgaaaggcca tgtgatgaac gccctgccgg
     4921 aagacgccaa agcgtgggta gaagaacacg ttggctttgt cgattccgcc gttgaccgca
     4981 tcgtaccgcc ttcggcttct gcaaccaacg atccgctgga agtgacggta gaaaccttca
     5041 gcgaatggat tgtcgataaa actcagttca aaggcgcact gccgaacatc ccaggcatgg
     5101 agttaaccga caacctgatg gcatttgtcg aacgtaaact cttcaccctg aacacgggtc
     5161 atgctataac cgcgtacctc ggaaaactgg ccggtcatca gaccattcgt gacgctattc
     5221 tcgacgagaa aatccgcgcg gtggtaaaag gtgcgatgga agaaagtggc gcggtactga
     5281 tcaagcgtta cggctttgac gctgacaagc atgcggcgta catccagaaa atcctcggtc
     5341 gttttgagaa cccgtatctg aaagatgatg tagagcgcgt aggccgtcag ccgctgcgta
     5401 aactgagcgc aggcgaccgt ctgatcaagc cactgctcgg tacgctggaa tacggcctcc
     5461 cgtacaaaaa tctgattcag ggaattgccg ctgcaatgca cttccgcagt gaagatgatc
     5521 cacaggctca ggaactggca gcactgatcg ccgacaaagg tccgcaggcg gcgctggcac
     5581 agatttccgg gcttgatgcc aacagcgagg ttgtatctga ggcggtaacc gcttataaag
     5641 caatgcaata atggtggacc aggcgcagga caccctgcgc ccgaataaca gattgtcaga
     5701 tatgcaggca acaatggaac aaacccaggc ctttgaaaac cgtgtgcttg agcgtctgaa
     5761 tgctggcaaa accgtgcgaa gctttctgat caccgccgtc gagcttttga ccgaggcggt
     5821 aaatcttctg gtgcttcagg tattccgcaa agacgattac gcggtgaagt atgctgtaga
     5881 accgttactc gacggcgacg gtccgctggg cgatctttct gtgcgtttaa aactcattta
     5941 cgggttgggc gtcattaacc gccaggaata cgaagatgcg gaactgctga tggcattgcg
     6001 tgaagagcta aatcacgacg gcaacgagta cgcctttacc gacgacgaaa tccttggacc
     6061 ctttggtgaa ctgcactgcg tggcggcgtt accaccgccg ccacagtttg aaccagcaga
     6121 ctccagtttg tatgcaatgc aaattcagcg ctatcaacag gctgtgcgat caacaatggt
     6181 cctttcactg acagagctga tttccagaat cagcttaaaa aaagccttcc aaaagtaagt
     6241 tctttcgctt acttctcctc tgcctgtttc ggccgataaa gccgacgata atactccaga
     6301 cgttgtagat ataacggcac gctttcatcg ggtattccgg acggaatcgc gttacgggga
     6361 ggaagttttt tcagatactc ccggaacgcc tggcttgatg ccatgaaatc tacggcttta
     6421 tcgataagaa gcggaacgtt ttcacctaat ttgcccatga tgatatctcc gtattacccc
     6481 gcgccgggaa tgcgcggccg ccaattttag tttaggttcc cggtaaagat gacattaagg
     6541 aaaacgtgct gaatcctcaa taatcatccg cttctgttta ttatcttttt tctatagatt
     6601 tcttaattaa tcaataaatt gcgtagtttt taagcatatt aatgattacg ttgcggaact
     6661 attgcaatgt catcagttgt gaacaaagca cctggtcgcg catactaggg gctataaatt
     6721 tatctttatc agaagccatc acatgaaaga agtcgaaaaa aacgaaatca aacgtctcag
     6781 cgatcgtctg gacgccatcc gccaccagca ggccgatctg tcgctggttg aagccgcaga
     6841 caaatatgcc gagctggaaa aagagaaagc cacgctggaa gcagaaattg cccgcctgcg
     6901 tgaagttcac agccagaaac tgagtaaaga agcacagaag ctgatgaaga tgccgtttca
     6961 gcgcgcaatt accaaaaaag agcaggctga tatgggcaag ctgaagaaaa gtgttcgcgg
     7021 actggtggtt gttcacccaa tgaccgcgct gggccgcgaa atgggcctgg aagagatgac
     7081 tggattctct aagaccgcgt tttaagtccc ctactggtca atacaactaa agtttcctca
     7141 tttttattcg ctcctgctgg catatgcagg a
//
LOCUS       DAPGOF010000217         7153 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.218, whole genome shotgun sequence.
ACCESSION   DAPGOF010000217 DAPGOF010000000
VERSION     DAPGOF010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.218"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            326..1240
                     /gene="shuT"
                     /locus_tag="RHV13_004250"
     CDS             326..1240
                     /gene="shuT"
                     /locus_tag="RHV13_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     ShuT"
                     /protein_id="HDV1243712.1"
                     /translation="MPRIITRPFLFSPLTLCISALASAAKSTVKRKKLFTALLALSWT
                     FSVTAAERIVVAGGSLTELIYAMGAGERVVGVDETTSYPPETAKLPHIGYWKQLSSEG
                     ILSLRPDSVITWQDAGPQIVLDQLRAQKVNVVTLPRVPATLEQMYANIRQLAKTLQVP
                     EQGDALVTQINQRLERVQQNVAAKKAPVKAMFILSAGGSAPQVAGKGSVADAILSLAG
                     AENVATHQQYKSYSAESLIAADPEVIVVTSQMVDGDINRLRSIAGITHTAAWKNQRII
                     TVDQNLILGMGPRIADVVESLHQQLWPQ"
     gene            1260..2597
                     /locus_tag="RHV13_004251"
     CDS             1260..2597
                     /locus_tag="RHV13_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HDV1243713.1"
                     /translation="MNAINTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLAQT
                     WQEMISQTVSPRKRLVYLHIPFCATHCTFCGFYQNRFNEDACAHYTDALIREIEMEAD
                     SVLHQSAPIHAVYFGGGTPSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAE
                     RIDACLDAGANRFSIGIQSFNSRIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLF
                     GLPGQDAQTWGEDLAIARDIGLDGVDLYALNVLPNTPLGKAVENGRTTVPSPAERRDL
                     YLQGCDFMDEAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAFGSGAGGSINGYSWM
                     NERNLQTWHESVAAGKKPLMMIMRNAERNAQWRHTLQSGVETARVPLDELTPHAEKLA
                     PLLAQWHQKGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPAIVREKP"
     gene            2610..3104
                     /gene="hutX"
                     /locus_tag="RHV13_004252"
     CDS             2610..3104
                     /gene="hutX"
                     /locus_tag="RHV13_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HDV1243714.1"
                     /translation="MSHVSLQEFLKTEPDGTLEVVAEQYNTTLLEVVKNLPSSTVVPG
                     DKFDTVWDTVCEWGNVTTLVHTADVILEFSGELPSGFHRHGYFNLRGKHGMSGHIKAE
                     NCTHIALIERKFMGMDTASILFFNKEGSAMLKIFLGRDDHRQLLSEQVSAFHTLAASL
                     KEHA"
     gene            3104..3727
                     /gene="chuY"
                     /locus_tag="RHV13_004253"
     CDS             3104..3727
                     /gene="chuY"
                     /locus_tag="RHV13_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobilin reductase"
                     /protein_id="HDV1243715.1"
                     /translation="MTPWLLFGAGGKGVGARTLELALAEQRPVVAVIRHADAATKLAQ
                     QGVQVFTGDACDASVVAAACRAAGPDALIISTMGGAQDYLAHRTVIDEAEKAGISRMI
                     LVTSLGCGDSWPFLSDRAKAAFGQAVREKTLAESWLQTSQLDYAILRPGGLLDGAATG
                     KAQRVQNQECHGFVRRADVAAHIHELANAPALNKQIYSLIEPDLKPA"
     gene            3812..4768
                     /gene="chuU"
                     /locus_tag="RHV13_004254"
     CDS             3812..4768
                     /gene="chuU"
                     /locus_tag="RHV13_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease ChuU"
                     /protein_id="HDV1243716.1"
                     /translation="MGLSLLLLALVLFGASQGALKISFDALFDEEYRDIWLNIRLPRV
                     LLAVLVGAALATAGVIMQGLFRNPMADPGLLGVSSGSALMVGVAIVLPFSFPVVLVLY
                     EQMMFAIAGSLVVCTIIFLITQRHRDGSMMQLLLAGIAINALCGAAIGILSYIGDEQQ
                     LRQLTLWMMGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQLGDEEAHYLGVNV
                     KRKRQQLLLVSSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRWLIPCSALAGACL
                     LLMADTLARTLVQPAEMPVGLLTSLLGGPYFMWLILRNRRIT"
     gene            4765..5535
                     /locus_tag="RHV13_004255"
     CDS             4765..5535
                     /locus_tag="RHV13_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HDV1243717.1"
                     /translation="MISAQNLVYSLQGRRLTDNVSLTFPGGEIVAILGPNGAGKSTLL
                     RQLTGYLQPDSGECRLFNKSLNEWSITELAKHRAVMRQNSHMAFPFSVQEVIQMGRHP
                     HRTGNQDNETAQIMALCDCQALANRDYRQLSGGEQQRVQLARLLVQLWEPTPSPKWLF
                     LDEPTSALDIHHQQHLFRLLRQLVHERQFNVCCVLHDLNLAARYADRIVLMQKGKVIA
                     NGKPQDVLTQQALTMLYGADITVLKDPANHSPLIVLDH"
     gene            complement(5587..6234)
                     /locus_tag="RHV13_004256"
     CDS             complement(5587..6234)
                     /locus_tag="RHV13_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDV1243718.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(6298..6624)
                     /gene="hdeB"
                     /locus_tag="RHV13_004257"
     CDS             complement(6298..6624)
                     /gene="hdeB"
                     /locus_tag="RHV13_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HDV1243719.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            complement(6740..7072)
                     /gene="hdeA"
                     /locus_tag="RHV13_004258"
     CDS             complement(6740..7072)
                     /gene="hdeA"
                     /locus_tag="RHV13_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HDV1243720.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
BASE COUNT         1797 a         1800 c         1727 g         1829 t
ORIGIN      
        1 aacagggggt tatgaaacct atcctgcgcc ctgtaaacca ccttgttttt atggttatcc
       61 ccccatgcct catcactaaa ttttgtcgtt aacagtgata attttttctt ctctttgctc
      121 tgcaagcctc gatatctctg ggttctcagc tttttcccgc gatgaaattt agtcggtaac
      181 aagaacgtta gctgacgaag catcttgata aaaatcaaaa ataaatacgc ttgtaacgcc
      241 ttgcgtattg aatcgacggt tgtatttcat atataactca aatgataatc atgatcattc
      301 tcaatttgat aagagttctc atattatgcc aaggatcatc accaggccgt ttcttttttc
      361 cccactgacg ttatgtattt cagccctggc ctctgccgcg aagagcacgg taaaacgaaa
      421 aaaattgttt acggcattac tggctcttag ctggactttt agcgtaacgg ctgccgaacg
      481 tatcgtggtc gcaggaggat cgctgacgga gctaatctac gcgatgggcg ctggcgaacg
      541 cgtggttggc gtcgatgaaa cgacatctta tccaccagaa accgccaaac tgccgcatat
      601 tggctactgg aaacaactca gcagcgaagg cattttgtca cttcgcccgg atagcgtaat
      661 tacctggcag gatgctggac cgcaaattgt gctcgaccag ctgcgggcgc agaaagtcaa
      721 cgtcgttacc ctgccgcgtg tgcctgccac ccttgagcag atgtacgcca acattcgtca
      781 actggcaaaa acgttacagg tacctgaaca aggggacgcg ctggtgacac agatcaacca
      841 acgcctggag cgagtacagc aaaacgtggc ggccaaaaaa gccccggtta aagccatgtt
      901 tattctctcc gcaggcggaa gtgcgccaca agttgccggt aaaggcagcg tcgcggacgc
      961 cattctttcg cttgccggag cagaaaacgt cgcaacccac cagcagtaca aaagctacag
     1021 tgcagaatca ctgattgcgg ctgatccaga agtgattgtc gtaacctcac aaatggttga
     1081 tggcgatatt aatcggctac gttctattgc cggaattacc cacactgccg catggaaaaa
     1141 ccagcgcatt atcaccgttg atcaaaacct gattctggga atgggtccgc gtattgctga
     1201 tgtcgttgag tctttacacc agcagctttg gccgcaataa atggatttaa atcagacgaa
     1261 tgaacgcaat taacaccctg gatctcacgc cacattttgc tttagacggt gatcagccat
     1321 ttaaagacag gcgcgccatg atgccgtttc gtggagccat tccggttgcc aaagagcaac
     1381 tggcacagac ctggcaagag atgatcagtc aaacggtatc accacgtaag cgactggttt
     1441 acctacacat tcctttttgt gcgacacact gcacgttttg tggtttttat cagaaccgtt
     1501 ttaatgaaga tgcgtgtgct cattataccg acgccctgat tcgtgaaata gaaatggaag
     1561 cggacagcgt attgcatcag tctgccccca tccacgcggt ctatttcggt ggaggtacgc
     1621 cttccgcgct ttcggcacac gatttggcca gaatcatcac cacattacga gaaaagctgc
     1681 cactggcccc tgattgtgaa atcactatcg aaggccgggt actgaatttt gacgctgagc
     1741 gaatcgatgc ttgccttgat gcaggagcaa accgcttctc aattggtatc cagtcgttta
     1801 acagcagaat ccgcaagaaa atggcccgca cctcggatgg cccaaccgcc attgcgttta
     1861 tggaaagcct ggtcaaacgt gaccgtgctg cggtggtctg tgacctgctg tttggcctgc
     1921 cgggtcagga tgcgcaaacc tggggagaag atctggctat tgctcgcgat atcggtctcg
     1981 acggcgtcga tctctatgcg ctcaatgtcc tgcccaatac cccgctgggc aaagccgtgg
     2041 aaaatgggcg cactaccgtg ccctctccgg cagaacgtcg cgatctttat ctgcaagggt
     2101 gtgacttcat ggacgaagcc ggttggcgct gtattagtaa cagccactgg ggccgtacca
     2161 cacgcgaacg caatctctat aacctgctga taaaacaagg tgccgattgt ctggcctttg
     2221 gttccggagc cggcggttcg attaatggtt actcctggat gaacgaacgc aatcttcaga
     2281 cctggcatga atccgtcgcg gcaggcaaaa aaccgctgat gatgatcatg cgtaacgccg
     2341 aacgtaatgc gcagtggcgt cataccttgc agtcaggtgt tgaaacagcg cgtgtaccgc
     2401 tggatgagct tacgccacat gccgaaaaac tcgcgccgtt actggctcaa tggcaccaaa
     2461 aaggcttaag tcgcgatgct tcaacttgcc tgcggctgac taatgaaggt cgtttctggg
     2521 caagcaatat tttgcagtct cttaatgaac taattcaggt acttaatgcg ccagcgattg
     2581 tgcgtgaaaa accataacag gagtgattta tgagccatgt ctcgttacaa gaatttttga
     2641 aaacggagcc ggacggtacg ctggaggtgg tagccgaaca atacaacacc acactgctgg
     2701 aggtagtgaa aaatctaccc tcctctaccg tagtgccagg cgataaattc gatactgtct
     2761 gggataccgt ctgcgaatgg ggcaacgtca ccacgttagt acatactgcc gatgtaatcc
     2821 tcgaatttag cggcgaactg ccttcgggtt tccatcgcca cggctatttc aacctacgcg
     2881 gaaagcacgg catgtccggg catatcaaag cagaaaactg cacgcatatt gccttaatcg
     2941 aacgtaaatt tatgggcatg gacaccgcgt ccattctctt tttcaataag gaaggcagcg
     3001 ccatgctgaa aatcttcctc ggtagggatg accatcgtca gctcctgagc gaacaagtca
     3061 gcgccttcca tactctggcg gcatcgttaa aggaacacgc ctgatgacgc cgtggctact
     3121 ctttggcgca ggcggaaaag gtgtgggtgc cagaacgctt gaactggcgc tggcggaaca
     3181 acgtccggtc gttgctgtca ttcgtcatgc cgatgccgcc acgaaactgg cgcaacaagg
     3241 cgtacaggtt tttacaggcg acgcctgtga tgccagcgta gtcgcggctg cttgccgcgc
     3301 agctggcccg gatgcgctta tcatctcaac aatgggagga gctcaggatt atctggcgca
     3361 cagaacagtg attgatgagg ccgaaaaggc cggcatcagt cgcatgattc tggtgacctc
     3421 tttgggatgc ggcgatagct ggccgttttt atcggatcgg gcaaaagccg cgtttggtca
     3481 ggcggtgcgc gaaaagacac tggcggaaag ctggctgcaa accagccagc tagactacgc
     3541 cattctccgc cctggtggtc tgcttgacgg cgcagcaaca ggcaaagcac aacgggtaca
     3601 aaatcaggag tgtcatggtt ttgtccgccg tgccgatgtc gctgcacaca ttcatgaact
     3661 ggcaaacgcg ccagcactca acaagcagat ctacagtctg attgaacctg acctgaaacc
     3721 ggcatgatac cccagcgcgg catgtccgcg ccgtaatgtc gtaacggagt tttgcgtgct
     3781 caaggattca ctctcttccg ccagggtctt tatggggcta tcactattat tgcttgccct
     3841 ggtgctgttc ggtgccagtc agggagcgtt aaagatcagt tttgatgccc tttttgatga
     3901 ggaataccgc gatatctggc tcaatattcg tctaccacgg gttttgctgg cggtgctggt
     3961 aggtgcagcg ttggcaaccg caggggtaat tatgcagggg ctttttcgca acccaatggc
     4021 tgacccagga ttacttggcg tcagtagcgg ttctgcatta atggttggcg ttgctattgt
     4081 gctgcccttc tccttcccag tggtgctggt gctctatgag caaatgatgt ttgccattgc
     4141 cggaagttta gtggtctgca ccatcatttt tctcatcacg cagcgccatc gcgatggcag
     4201 catgatgcaa ttattactcg ccggtatcgc catcaatgcc ctgtgcggcg cagcgatcgg
     4261 catcctgagc tatatcggcg atgagcagca gctacgacaa ctcacattgt ggatgatggg
     4321 caatcttgga caggcgcaat ggccgacgtt attggttgcc agttcattca tccttccggc
     4381 cattatcgca acaacttgtc tcgccggaac gctgaattta ctgcagctcg gtgatgaaga
     4441 agcccattac ctcggcgtga acgttaagcg taaacgccag caattactgt tagtgagctc
     4501 actgctcgtt ggtgctgccg tatcggtaag cggcattatc ggctttattg gcctggtgat
     4561 cccgcatctg attcgcatga ctaccggagc aaatcaccgc tggctcatcc cttgttccgc
     4621 cctcgccgga gcctgtttat tgctgatggc agacacgctt gcccgcacgc tggtacagcc
     4681 agcagaaatg cccgtgggat tattaaccag cctgcttggt ggcccttatt ttatgtggtt
     4741 gattctgcga aatcggagga tcacatgatc agcgcccaga atctagttta tagccttcag
     4801 gggcggcgtc tgactgataa tgtctcgctg acttttcccg gtggggaaat tgttgccatt
     4861 ctcggcccta atggtgcggg aaaatctacg ctgcttcgcc agttaaccgg ctatcttcaa
     4921 cctgatagcg gcgaatgtcg gctgtttaat aagtcattaa atgaatggtc gattactgaa
     4981 ctggcgaaac accgggccgt gatgcgtcaa aatagccaca tggcgtttcc attcagcgta
     5041 caagaagtta tccagatggg tcggcatccg catcgtactg gtaatcagga taatgaaaca
     5101 gcacaaataa tggcgttatg tgactgtcag gcgttagcaa accgcgacta tcggcaatta
     5161 tccggtggtg aacaacagcg cgtacaatta gcgcgcctgc tggtgcaact ctgggaacct
     5221 actccctcac cgaaatggtt atttcttgat gaaccaactt cggcgctgga tattcaccat
     5281 cagcagcact tgttccgttt attacgccag ttagttcacg agcggcagtt caatgtttgc
     5341 tgcgtattgc acgatctaaa tcttgccgcg cgttatgcag accgtattgt attaatgcaa
     5401 aaaggaaagg ttatcgcaaa tggtaaacca caggacgtat tgacacagca ggcgctgaca
     5461 atgctctacg gcgcggatat taccgtactt aaagatcctg ccaaccattc accactgatc
     5521 gtcctcgatc attaaaagca acaggagtac cgcactggta ctcctgcgaa taaataacta
     5581 tacagattag ctaattgaaa cgcctttcac ccctgccata ccttttaata atcgcaacag
     5641 atcttctatc gacgttgtgg catgaagctg gatatcaatg gcgaccactt catgatcttc
     5701 attttcctgc aacgaaacta aatcagtttt tattttttgc tgcttaaacc agtcgagcat
     5761 tgataccgta ttaccgttca ctaatgtcag ctgtaaatgg taatttttgt tcatcaggcg
     5821 gaaggttaat tgatggaaga cttccagcac cagcaaggtc atcactgaac cataaatacc
     5881 cagttcgtac ataccactgc caataaccat acctatggcg gcggtcaccc agatatccgc
     5941 tgccgtcgtc aaacctacaa tattttggtt acgaacgaga atgttacctg caccgataaa
     6001 cccgacgccc gtcaccacct gagcagcaat acggctgggg tccagtccga cgtgatctaa
     6061 agacagcacg tcagcaaaac catatttcga aacaatcata aacagggcgc ttcccatgcc
     6121 aattaataca tgtgtgcgta accctgctcc tttgccgcgc atttgccttt ccatgccaat
     6181 agcgccacag gcaattgccg cgagtataag acgtataata aattctgctg tcatataaaa
     6241 atgcaaaaag gagcagcaag atggctcaac ttgctactcc tttttacttg tacctcatta
     6301 attcggcaag tcattagatg cttgattttt gaaggtatac aaatttttct gcggattttt
     6361 cttgcagtat tcgatcactt taggaatttg agtgagatcg gtttcattta aagtaacggt
     6421 atcgccacct ttatatactg tttcttcatg cagcatccac catgcaaccg gggtcattgc
     6481 ttttggattc agatcaataa attcctggca ggtcatatct ttagcggatt cattggctgc
     6541 cagcgcagat tgtgcgttca ccagtgacaa agccgctaca gcgcccataa aaataaacgc
     6601 tttacggaga gatgaaatat tcattttgta acccattcaa tatagagatt atatatgaac
     6661 aggaggaata gctgctttta aagaagatat tccggcctgt tcaatatgca aggaagtacg
     6721 atgtaacgca ttcccggaat tacatatctt tcttaatttt gtcccattcg cctttaactt
     6781 tatctttaaa gttggcttgt ttatcctgag tacaagcctg aacgatagct ggggttacgg
     6841 ttgcaatacc ctgaacatct aaaaccgcat cttctggttt atctttgttg ttcagcgctt
     6901 cagcaaaacc aactgcagtt ggctggaagg attcgtccac agccaggaaa tcttcacagg
     6961 tccaggagtt gaccggtttt ttgttatcag ctgctttttg cgcatccgct gcattgctca
     7021 caactggcag aagaagcaga ccaccaagaa taacgcctaa tacttttttc atcgtaatat
     7081 cctcaactat aaagtgaaag agccgtcacg aatcaatttc gacactgagg ttataacctg
     7141 gttttctgta tat
//
LOCUS       DAPGOF010000218         7079 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.219, whole genome shotgun sequence.
ACCESSION   DAPGOF010000218 DAPGOF010000000
VERSION     DAPGOF010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7079
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.219"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            62..2446
                     /locus_tag="RHV13_004259"
     CDS             62..2446
                     /locus_tag="RHV13_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HDV1243721.1"
                     /translation="MFIEKSDSFLELPSEVIFPEAANAAILKHDKWADVWETLTTDAD
                     LNYTDEKETVSLSSLVTSATSAIYQAITDGWTMCVGYSGGKDSHSLLHLFLMALIRAV
                     RNGTNISEHHFIQMSDTLIENPEMHYQASQVLNQLRMFIAEHQLPVTVLVAQPSLTQS
                     WVGRILTGRGLPTWTNSSTRQCSTDYKIAPLRKAKARYLKNAPASVRSRVCLMLGSRD
                     DESARRAGNILKMGGQARKVTLTEHGGELYPVKEWRTQDIWSFLMACGSESRFPLPSF
                     MPDNFSLATLYKDATGECIWSPEKPTRTSACGARYGCSLCTAVGVDHSMETLLRTDPE
                     KYGYQAGLSRLQRFLSKIQYDWSLRDYIGRKVFEGGYVRLQPNIFSSSLTERLFHVCC
                     SLDYVEARRAAKHRRKLLSGEVDDTAYNRRMAEPQFRLVHEANVIHVDFLWSLHCFNP
                     RPFRAIEIYRRVWEEADLDLLEDEPDMLPVARTPMPAPLWMKLPGGRFGTAYDGLTDT
                     LPLMTYFDGQADPRASRSLKTGESSSVVVAFEEEDELTVEEDTASWIIWHEYDGLRQS
                     IADGEFTPTTAAQYLLRYGAVRISKGKGAVYHRLAQRGQTFSRLGIGERVSLPELVAS
                     RRFKILSDTAYRQVVARKLRGQIKKFRFWACVAACVQLHVHNKTALGERILTLLEGER
                     EQQQGAIQAKLKAGMMDAVLTLCNQRLRVKENTNQPEEFRYYRAVRARFMRHLSECLK
                     PENGGVIRDVIWELRVLSSAHGTTKTGFYYVDSNRPTAKGLLNRLLMRMVRQVV"
     gene            2696..2935
                     /locus_tag="RHV13_004260"
     CDS             2696..2935
                     /locus_tag="RHV13_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243722.1"
                     /translation="MQNQNTPAQEKHTVYLFNAEHADKTAAKQAGKLFTLDIEHPMTL
                     NDLSLLCESVADAVGAPGGIKYEITTEPNGVQEEY"
     gene            2998..3309
                     /locus_tag="RHV13_004261"
     CDS             2998..3309
                     /locus_tag="RHV13_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000172889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243723.1"
                     /translation="MTLKMGSPRTSKPEGPFVLITFEGDEFLFDDRHNVVKINGRPKK
                     MDVKRNYFESDLGEGKAKYWTLNIEEAHRFNSIDEATEQLCKLNRPHLIKIRKVEGVS
                     E"
     gene            3306..3494
                     /locus_tag="RHV13_004262"
     CDS             3306..3494
                     /locus_tag="RHV13_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243724.1"
                     /translation="MTKEMFLRILNEAQARVDNDSLPLDVRIRSRTTVNDCVIRADKE
                     GWPIEYKQKVWVEAVSGC"
     gene            3555..4088
                     /locus_tag="RHV13_004263"
     CDS             3555..4088
                     /locus_tag="RHV13_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDV1243725.1"
                     /translation="MVKVININGNLVELPEPSAKLSKAESPDGRFSKPKNKISKIQRA
                     ELRMKFGGRCAYCGCKLPEKGWHADHVEPVRRDFELVRAPVGSGVTHVARSTGKVMHP
                     ELHAIENLFPSCAPCNLFKGAFSVEGMRNEITKQVERARAYSVNFRTAERFGLLHIVV
                     KPVVFWFEQYNEQKQNE"
     gene            4203..4811
                     /locus_tag="RHV13_004264"
     CDS             4203..4811
                     /locus_tag="RHV13_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243726.1"
                     /translation="MYLHLVPRILHHMKNKCTLMSVSVPELSLELKADSLVAMKPYPN
                     KTYHVGMLKGRRALNGFLVKSPRTLADFTMITLWEIDGFGEISHTVKTLVQDNDYDLV
                     SHDVLLAHAYHQTEEGLGYRVHPSYDSLAPVDFEPTMQSRYIKESDLSHDVWETYSWG
                     EFLRSREETFLAMTISSSRLNHPAFIRGNRLPQTDQAIIISS"
     gene            5000..5287
                     /locus_tag="RHV13_004265"
                     /pseudo
     CDS             5000..5287
                     /locus_tag="RHV13_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            5344..6536
                     /locus_tag="RHV13_004266"
     CDS             join(5344..5620,5620..6536)
                     /locus_tag="RHV13_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612052.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISKpn18 family transposase"
                     /protein_id="HDV1243727.1"
                     /translation="MTDKTTDTTKNRRTFDPAFKLQVARMVREQGVSVSQVCREMKLT
                     ESVVRRWVHQLDDESAGRPGIGKPLTAEQQRIRELEAEVRRLKSDNELFKKSFGLLRT
                     GNKVIHDVVTELAKQAPIKQVCQALDISRSGYYAARRRVNTPKPICQDSVHAQAVFLA
                     SGRTYGSRRLSAALRAQGHDVGRHRCRTLMKACGLKACWRRKFAHTTDSKHHLPVAEN
                     VLNRQFEPEAPNTAWVCDVTYIRTDSGWLYLAAVLDLYSRRIVGWSMAPTMPAQLVCD
                     ALSMAISVRQPAPGLLVHSDRGSQYASAEHRQLLENHGLVLSMSRKGNCWDNAVMERF
                     FLNLKMERVWQTRYANHTEAKQDITDYIVNFYNSTRLHSKLGYRSPAQYETLASPAAQ
                     LPACA"
     gene            6582..7079
                     /locus_tag="RHV13_004267"
                     /pseudo
     CDS             6582..7079
                     /locus_tag="RHV13_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
BASE COUNT         1933 a         1660 c         1733 g         1753 t
ORIGIN      
        1 tattttaaag ttctaataaa aatatcagtg atgcagcata tgccgtggtt caggaaaata
       61 tatgtttatt gaaaaaagtg actcattcct tgaattaccc tcagaagtta tttttcctga
      121 ggcggctaat gctgccattt tgaaacatga taaatgggct gatgtttggg agaccctgac
      181 aaccgatgcc gatctgaact ataccgatga aaaggagact gtgagtcttt cttctctggt
      241 cacgtcagcc acatctgcta tttatcaggc tattacggac ggatggacga tgtgcgtggg
      301 atacagtggc ggtaaagact cccattctct tcttcaccta tttctgatgg cattgatcag
      361 ggcagtacgt aacggcacaa atatcagcga acatcatttc attcagatgt ccgatacgct
      421 gatcgaaaac cctgagatgc actatcaggc ttcacaggtt ctcaaccagc ttagaatgtt
      481 cattgctgaa caccagctac cggtcacagt ccttgtcgcc caaccgtctc ttacgcagag
      541 ctgggtaggc cgcattctga ctggacgtgg acttccgacc tggactaatt cttcaacccg
      601 tcagtgctca accgactaca aaatcgcccc cctgcgcaag gccaaagctc gttatctaaa
      661 aaacgccccg gcatccgtac gaagcagggt atgcctgatg ctgggctcca gggatgatga
      721 aagtgcccga agggccggaa atatcctcaa aatgggaggg caggcgagga aagtaaccct
      781 gactgagcac ggtggtgaac tctatccagt aaaggagtgg cgcactcagg acatctggtc
      841 ttttctaatg gcctgtggtt ccgaatcccg cttcccttta ccaagcttta tgccggataa
      901 cttcagcctg gcaacgcttt ataaagacgc caccggagag tgtatctggt cgccggagaa
      961 acccacgcga acctcagctt gtggggctag atatgggtgc tcgctttgca cagccgtagg
     1021 ggttgaccat tcaatggaaa cgctccttcg cactgaccct gagaaatatg gttatcaggc
     1081 aggcctaagt cgtcttcagc gatttctttc taagatccag tacgactgga gcctgcggga
     1141 ttatatcggt cgtaaagtgt tcgaaggcgg gtatgtgcgt cttcaaccca atatcttttc
     1201 ttcgtccctg actgaacgtc tttttcatgt ctgttgcagc cttgattatg ttgaagccag
     1261 acgtgcggca aagcatcgca gaaagctact ttccggcgag gtggacgata cggcttataa
     1321 ccgtcgtatg gctgagccac aatttcggct tgtacatgaa gcaaacgtga tccacgttga
     1381 tttcctctgg tcgttgcact gtttcaaccc tcgcccgttt cgggcaattg agatctatcg
     1441 cagagtctgg gaagaggccg atctggacct tcttgaggat gagcctgaca tgctgcctgt
     1501 ggcccgtaca ccaatgccag ctccactctg gatgaaactg cccggaggcc ggttcggtac
     1561 agcttatgat ggactgactg atacgttgcc gctgatgacc tattttgacg ggcaggctga
     1621 ccccagggct tcacgtagcc taaagaccgg agagagcagt tctgtcgtcg tggcattcga
     1681 ggaagaggat gagctgactg ttgaagaaga tacggcgtcc tggattatct ggcatgaata
     1741 cgacgggtta cgccagagca tcgctgacgg tgagtttact ccaacgactg ctgctcagta
     1801 tcttcttcgt tatggcgcgg tccgaatttc aaaagggaag ggcgctgtct atcatcgtct
     1861 cgcacaacgg ggacaaacct tttcccgcct ggggataggc gaaagagtgt cgcttccaga
     1921 gctggttgcc agtcggcgtt ttaaaatact cagcgatact gcataccgtc aggttgtagc
     1981 ccggaaattg cgtggacaga taaaaaagtt cagattctgg gcatgcgtag ctgcctgtgt
     2041 acagttgcac gtacacaaca agacggcact cggagaaaga atcttaacgt tactcgaagg
     2101 tgagagggaa cagcaacaag gtgcgatcca ggcgaagctg aaggcgggaa tgatggatgc
     2161 agttctaact ctgtgtaacc agcgactccg ggtgaaagaa aatactaacc agccagagga
     2221 gttccgctat taccgtgccg taagagctcg tttcatgcgc catttatctg aatgccttaa
     2281 gccagaaaat ggcggcgtta tccgtgacgt tatctgggaa ctgagggtgc tgagcagtgc
     2341 tcacggtacg accaaaacag gtttttacta tgtcgatagc aatcgcccga cagcgaaagg
     2401 actgttaaac cgccttctga tgaggatggt caggcaggtc gtataagcgt ctgaaacgcg
     2461 aaacacacga aaatgaaggg gttatacaaa aaaaacttag ctttgatcgt gatgtaatgt
     2521 ggacatcatg tccaagcgtt gctgcatcca gaaaagtaac tttaatcctg ttgatacagc
     2581 ctttgcatta cctgtttacg catagcctgg aaacgttcta attaaaccag acaaatataa
     2641 atttaaatta attcactcac ccccggaatc ggggaactaa acaaaaggat catcaatgca
     2701 aaatcaaaat acccccgctc aggaaaaaca caccgtatac ctttttaatg ctgaacatgc
     2761 tgacaagaca gccgccaagc aagccgggaa actcttcacg ctggacattg aacacccaat
     2821 gaccctcaac gatctctcgc tgctgtgtga gtcggtcgcg gacgctgtgg gagcacctgg
     2881 cggtattaaa tatgaaatca caacagagcc taatggggtt caggaggaat actgaatcag
     2941 tgtggtgtga gtccatttta atttgaggtt tcatgagttc tatcgagagg ctgaagtatg
     3001 actttgaaaa tgggaagtcc ccggacttcc aagcctgagg ggccatttgt attaatcact
     3061 tttgaggggg acgaatttct ttttgatgac agacataatg tcgtcaagat aaatggcagg
     3121 cctaaaaaaa tggatgtaaa gcgaaattat tttgagtccg atctgggaga agggaaagcg
     3181 aaatactgga ctttaaatat cgaagaggct catcggttca attcgattga tgaagcgaca
     3241 gaacaacttt gtaaactgaa tcgccctcat ctgatcaaaa ttcgcaaagt tgagggggta
     3301 tctgaatgac caaagagatg tttttacgca ttttaaatga ggcacaggct cgtgtggata
     3361 acgattcgct tccgcttgat gttcgtatcc ggtcacgcac cacggtaaat gattgcgtca
     3421 tccgggcaga taaagagggg tggcctattg agtataaaca gaaggtctgg gtagaggcag
     3481 tcagtggctg ttaacttcag cggttactta agccagctcc tgtgcccgtg agattagtaa
     3541 aggcggaaaa tgatatggtc aaggtgataa atatcaacgg gaatttagtt gagttacctg
     3601 agccctcagc aaagttatcc aaagcggaat cacctgatgg taggttctca aaacctaaaa
     3661 ataagatctc taaaatacag cgagctgagc tgcgaatgaa atttgggggg cgttgtgcct
     3721 attgtggttg caagcttcct gaaaaagggt ggcatgccga tcatgttgag cctgttcgac
     3781 gcgactttga actggtgaga gccccggtcg gcagcggcgt aactcatgtt gcccgaagta
     3841 cgggtaaggt tatgcaccct gaactgcatg caatagaaaa cttatttcca tcatgcgctc
     3901 cctgcaacct ttttaaagga gcttttagtg ttgaaggaat gagaaatgaa attaccaaac
     3961 aggttgaacg ggctcgcgca tatagcgtga atttccgtac cgcagaacgt tttggtttgc
     4021 ttcatattgt agtaaaaccg gtcgtcttct ggtttgagca gtataacgaa cagaaacaaa
     4081 acgaataatt tctcagagac ataaaacaag tcaatcaaca gtggttaacc aattaaaaaa
     4141 gtctattaac tattttgatg ctgctgagga tctgccagtt atatccataa aaggagaaaa
     4201 tgatgtatct gcatcttgtg ccacgtattc ttcaccatat gaaaaacaag tgtactctca
     4261 tgtccgtgtc tgtccctgaa ttgtcacttg agcttaaagc tgattcgctg gtggcaatga
     4321 aaccctatcc caacaagacc taccacgttg gcatgctgaa ggggcgacga gcattaaatg
     4381 gctttctggt aaagagccca cgaactttag ctgacttcac aatgataacg ctctgggaaa
     4441 tagacggctt tggggaaatt agtcataccg taaaaacgct cgttcaggac aacgactatg
     4501 acctggtctc ccacgatgtc cttttggcac atgcatacca tcagacggag gagggattgg
     4561 ggtaccgtgt tcatccatcc tatgacagtt tagcccctgt cgattttgag ccaacaatgc
     4621 aatcccggta cataaaggaa tccgaccttt ctcacgacgt atgggaaacc tattcatggg
     4681 gggaatttct gcgttcgcgt gaagagacat ttctggcgat gaccatttct tcttctcgtt
     4741 taaaccaccc tgcatttatc agaggaaacc ggcttcctca aactgaccag gccatcatta
     4801 ttagttccta actctgcctg gaataaaagt cagacgacgg gtcagatttc tagcatctaa
     4861 ttaaatgaat gcccggtaag ccgggctttg tcctgatgaa tcccctaatg attttggtaa
     4921 aaatcattaa gttaaggtgg atacacatct tgtcatatga tcaaatggtt tcgcgaaaaa
     4981 tcaataatca gacaacaaga tgtgcgaact cgatatttta cacgactctc tttaccaatt
     5041 ctgccccgaa ttacacttaa aacgactcaa cagcttaacg ttggcttgcc acgcattact
     5101 tgactgtaaa actctcactc ttaccgaact tggccgtaac ctgccaacca aagcgagaac
     5161 aaaacataac atcaaacgaa tcgaccgatt gttaggtaat cgtcacctcc acaaagagcg
     5221 actcgctgta taccgttggc atgctagctt tatctgttcg ggcaatacga tgcccattgt
     5281 tgtggtcaaa tttaaatgga cactttgatc gggtgttttc cccccgacaa ggaacatgaa
     5341 gagatgaccg acaagacaac cgacacaacc aagaatcgcc gaacctttga tccagcgttc
     5401 aagttacagg tggcgcgcat ggtgcgcgag caaggcgtca gcgtcagcca agtctgccgc
     5461 gaaatgaagc tgaccgaaag tgtggtccga cgctgggtac accaactgga tgacgagtca
     5521 gccggcaggc cggggattgg taaaccgtta acggctgagc agcaacgtat tcgagaactg
     5581 gaagccgaag tcaggcgact aaaatcagac aatgaattat taaaaaaagc ttcggccttc
     5641 ttcgcacggg aaataaagtg atccacgatg ttgtcactga gttagcgaag caggccccga
     5701 tcaagcaggt ctgtcaggcg ttggacatca gtcgctctgg ttactacgca gcgcgtcgca
     5761 gagtcaatac acccaaacct atttgccagg acagcgtgca tgctcaggcg gtttttcttg
     5821 ccagtggtcg cacctatggc agtcgtcgtt tgagtgcagc cctgcgtgcc cagggccatg
     5881 atgttggacg tcatcgctgt cgtaccttga tgaaagcctg tggactcaag gcctgttggc
     5941 gtcgcaagtt cgctcatacc accgatagca aacaccattt gccggtggca gagaacgtgc
     6001 tcaaccgtca gtttgagcct gaagcaccca atacggcctg ggtgtgcgat gtgacgtaca
     6061 ttcggaccga cagcggttgg ctatatctgg ccgctgtgct ggatctgtat tcacgtcgca
     6121 tcgtgggttg gtccatggca ccgaccatgc ctgcacagct ggtgtgcgat gcgttgagca
     6181 tggctatttc ggttcgccaa ccggcaccgg gtttattggt tcattcggac cggggcagtc
     6241 aatatgccag tgctgaacac cggcaattac ttgaaaacca tggtctggta ctcagcatga
     6301 gccgcaaggg taattgctgg gacaatgccg tgatggagcg gttctttctg aatttgaaaa
     6361 tggagcgtgt ctggcagact cggtatgcca accatacaga agccaaacaa gacatcaccg
     6421 actacatcgt taacttttat aacagtaccc gattgcattc aaaacttggc taccgatcac
     6481 ccgcacagta cgaaacgctg gccagccctg ccgcccagtt gcccgcctgt gcataaccct
     6541 accatctgat cggcgtgtcc aaaaaaactt gaccacaaca cattgtactt gttgactggt
     6601 ctgatattcg tgagcaaaaa cgacttatgg tattgcgagc ttcagtcgca ctacacggtc
     6661 gttctgttac tctttatgag aaagcgttcc cgctttcaga gcaatgttca aagaaagctc
     6721 atgaccaatt tctagccgac cttgcgagca ttctaccgag taacaccaca ccgctcattg
     6781 tcagtgatgc tggctttaaa gtgccatggt ataaatccgt tgagaagctg ggttggtact
     6841 ggttaagtcg agtaagagga aaagtacaat atgcagacct aggagcggaa aactggaaac
     6901 ctatcagcaa cttacatgat atgtcatcta gtcactcaaa gactttaggc tataagaggc
     6961 tgactaaaag caatccaatc tcatgccaaa ttctattgta taaatctcgc tctaaaggcc
     7021 gaaaaaatca gcgctcgaca cggactcatt gtcaccaccc gtcacctaaa atctactca
//
LOCUS       DAPGOF010000219         7068 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.220, whole genome shotgun sequence.
ACCESSION   DAPGOF010000219 DAPGOF010000000
VERSION     DAPGOF010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.220"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            5..190
                     /locus_tag="RHV13_004268"
     CDS             5..190
                     /locus_tag="RHV13_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243728.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKVSVRQYYLKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
     gene            151..270
                     /locus_tag="RHV13_004269"
     CDS             151..270
                     /locus_tag="RHV13_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDV1243729.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            305..649
                     /locus_tag="RHV13_004270"
                     /pseudo
     CDS             305..649
                     /locus_tag="RHV13_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(1021..1287)
                     /gene="minE"
                     /locus_tag="RHV13_004271"
     CDS             complement(1021..1287)
                     /gene="minE"
                     /locus_tag="RHV13_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HDV1243730.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(1291..2103)
                     /gene="minD"
                     /locus_tag="RHV13_004272"
     CDS             complement(1291..2103)
                     /gene="minD"
                     /locus_tag="RHV13_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HDV1243731.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2127..2822)
                     /gene="minC"
                     /locus_tag="RHV13_004273"
     CDS             complement(2127..2822)
                     /gene="minC"
                     /locus_tag="RHV13_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HDV1243732.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            3341..3709
                     /locus_tag="RHV13_004274"
     CDS             3341..3709
                     /locus_tag="RHV13_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HDV1243733.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMVSGNDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(3829..4230)
                     /locus_tag="RHV13_004275"
     CDS             complement(3829..4230)
                     /locus_tag="RHV13_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HDV1243734.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            4472..4765
                     /locus_tag="RHV13_004276"
     CDS             4472..4765
                     /locus_tag="RHV13_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HDV1243735.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELIKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            4837..5496
                     /locus_tag="RHV13_004277"
     CDS             4837..5496
                     /locus_tag="RHV13_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDV1243736.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            5588..6034
                     /locus_tag="RHV13_004278"
     CDS             5588..6034
                     /locus_tag="RHV13_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HDV1243737.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(6240..>7068)
                     /gene="hlyE"
                     /locus_tag="RHV13_004279"
     CDS             complement(6240..>7068)
                     /gene="hlyE"
                     /locus_tag="RHV13_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HDV1243738.1"
                     /translation="KYLDQVIPWQTFDETIKELSRFKQEYSQAASVLVGDIKTLLMDS
                     QDKYFEATQTVYEWCGVATQLLAAYILLFDEYNEKKASAQKDILIKVLDDGITKLNEA
                     QKSLLVSSQSFNNASGKLLALDSQLTNDFSEKSSYFQSQVDKIRKEAYAGAAAGVVAG
                     PFGLIISYSIAAGVVEGKLIPELKDKLKSVQSFFTTLSNTVKQANKDIDAAKLKLTTE
                     IAAIGEIKTETETTRFYVDYDDLMLSLLKEAAKKMINTCNEYQKRHGKKTLFEVPEV"
BASE COUNT         1817 a         1571 c         1676 g         2004 t
ORIGIN      
        1 ttatgtgagc agaagatatt catcagcaac gattacatta gtcattttat tttgccgacg
       61 gcctcattgt cgaaaagtca gcgtacgaca gtattatctg aaaagagtga ttttttatcc
      121 aactacactt cagcgcactg cgtttaaaaa atgcctcttt cttatgcggg atatcatcat
      181 ttcatcatga tgtctttggt gagcggtgaa cacaatacac ctgcgctgtc tcttcaggat
      241 gaatcccctg gtctggtgcc tgcgggctga tgttgcagcc gagctaaggt cacttagacg
      301 ctactatcat ttatccaatg gcatggaatc gaaatcagtc gatacccgca gtatatatcg
      361 tgaactgggt gcaacgctga gttacaacat gcgtctgggg aacggtatgg aaattgagcc
      421 gtggctgaag gcggctgtgc gcaaagaatt tgtcgatgat aaccgggtga aggtgaataa
      481 tgacggtaat ttcgtcaatg atttgtcggg cagacgtgga atataccagg caggtattaa
      541 agcctcattc agcagtacgt taagcgggta tctcggggtg gggtatagcc atggtgctgg
      601 tgtggaatcc ccgtggaatg cggtagctgg tgtgaactgg tcgttctgac catcaacgat
      661 taaactgcac ttcggcacag ttttcgttta caggatgtta aaagagaaaa ttctggggta
      721 aaaaaagccc gccagttacg gcgggaaacc tcatcctatg ggagaacaat gaataatgaa
      781 attgcggggt tatcatctcc cagcatatcc acactaacaa taaggttatt tactcaacca
      841 tgcataaata ttttgttttg tgcgtgggaa cagccttaag gtgtaaaggg ggaggtggaa
      901 atagcaatga ggagtatcag caagaatact cgccgcttta ccacaacgtc gatgagaggg
      961 atgaaaaact caaggcagag atgactctgc cttgaagata aatgcgcttt tacagcgggc
     1021 ctatttcagc tcttctgctt ccggcaaggt cacgttcagc tcaagaatag aaatatcgcc
     1081 atctttttgc tcaagctgta cggttaccat ctcaggatca atttgtacgt atttacaaat
     1141 gacctcaaga atatctttac gcaactgcgg cagataatgc ggttctgcat cgctgcgacg
     1201 gcgttcagca acaataatct gcagccgttc ttttgcaatg ttggctgtgt ttttcttccg
     1261 cgagagaaag aaatcgagta atgccataac ttatcctccg aacaagcgtt tgaggaagcc
     1321 tttcttctct tcttcaatga agcggaaagg acgttcttct cccaacagac gttctacggt
     1381 atctgcgtag gctttacccg catcggcgtt aatgtcgaga atgaccggtt caccctggtt
     1441 agaggcgcgc aatactgatt gatcctctgg gatcacgccg acgagtttga tgcgcaggat
     1501 ctccagcaca tcttccatgc tcagcatgtc acctctgctt acgcggcctg ggttatagcg
     1561 cgttaacagc aggtgctctt taataggctc ttcgccattt tctgcgcggc gtgatttcga
     1621 cgccagaatg cctaaaatac ggtcagagtc gcgtactgaa gagacttccg ggttggtggt
     1681 aataatggct tcgtctgcaa aatagagtgc cattaacgca ccggtttcaa tccctgccgg
     1741 ggagtcacaa acgataaatt caaaatccat cgctttcaga tcatcaagaa ctttggcgac
     1801 cccttcacgg gtgagggcat ctttatcgcg tgtttgcgat gccggcagaa tatagagatt
     1861 ttcagtacgc ttatctttaa ttaacgcctg atttagcgtt gcatcgccct gaatgacgtt
     1921 gacgaaatcg taaacgaccc ggcgttcaca acccataatc aggtcgagat tacgcaggcc
     1981 gatatcaaaa tctatcacga cagttttctt tcccttctgg gccaaaccag tggcgatggc
     2041 cgcgctggag gttgtcttac caacaccccc tttgcccgaa gtaacaacaa taatgcgtgc
     2101 catagaaatt ccttgttaaa aagggatcaa tttaacggtt gaacggtcaa agcgttttcg
     2161 actaactgca gtcgcgccgc tttgccataa aattctgctg ggatttgatc actcagccag
     2221 tattcacctg cgatggacac cagttccgcc atcaggttcg tacaaaatat ttgcgtttcc
     2281 cggtcaccac ttgcccctgc cagcgcacga ccgcgcatca tgccatagac atgaatgtta
     2341 ccatcggcaa ttaattcggc cccagcgcta acgtggcttg taacaatcag atcacattgt
     2401 ggagcataaa tacgctgacc ggaacgcacc ggggtatcta ttaaacgcgt ttttgtgacc
     2461 ggcgttgtat tttgcgctgg agcctgcggt gcgggagctg gacgtggcgc tttttccttt
     2521 ccttccgtca ggataggcag ccccattttt tcaatttcgg ctttaagttg cgcatctttg
     2581 cagccactta cgccaataac ccgcaaaccg gttgccgaaa ccgccttatg catcgctgac
     2641 cagtttaccg ggtcttccag tgcactgacg ttgagtacaa cgggggcatg ttttaaaaat
     2701 gcgggggcct gagcgatttt gtcttccagc gcctgatgga taaccttagg ttctgcctca
     2761 tgcagatgaa ccacagataa agtgaagcta ctgcctttaa gctcgattgg cgtgtttgac
     2821 atcctggcct tactcaatta gctattaatc atcgccagcg cgcgatgatg ttccgaagac
     2881 tataaggcat gttatagtct ggattatatt gaggcaagtc accctcccat ttattcagag
     2941 taaaagtcta ttctgtgata aatggcgctg attcatagct taaaaaatac ccttgtcaat
     3001 caacccattg ccgtcgtact tttgattctt cttatttacg cttccttttt ccgcacccta
     3061 atcgtgacag catcttcaga cctgtttaaa tatccagtat tgaggcaagt gtattttttc
     3121 accacgccag gctttcttaa ctttccattt ttgcagcatc attaaagaac agatacccgt
     3181 tatcttaata tccgccacag aattgctcta tcatagaaac cgcagaagac gtctgatact
     3241 tctgtcaggt catcgtaact cgtccaggtc agcggttttg attacggatt gatgatgaaa
     3301 tgagcataag tgttgtacgg ttggattatc aggatgtgct atgaatatga tgcgtatttt
     3361 ttatatcgga ttgtcaggtg tggggatgat gttctcatca atggtgtctg gaaatgacgc
     3421 tggcggactt caatctccgg cgtgcggcgt tgtttgcgac ccgtatattt gtgtgaactc
     3481 agatggcatt tccccagagt taacaaggaa atatctcggc gaaaaagccg ctgaaaactt
     3541 acaatcatta caaggctacg atcccagcga atttacattc gctaacggtg tattttgcga
     3601 tgttaaagaa aaattatgtc gtgatgatcg ttattttggt gtggatggta agcggagtgg
     3661 aaaaatcaat caaaccacca caaagatgtt atttatgtgt cgtgagtgat gccttgttta
     3721 cattatgttc tattcggcat tgtctgctat tcgtcattaa cctcttgagc gacagagagg
     3781 agtggggcgt taacgtttat gacggggaga gtccccgtcg gttgacattt acttaatttg
     3841 aatatcgacg ttatattttt tagttttgtt tttacgggcg tcgtttcggg tttgcagcac
     3901 tcgcagctca tatttgccag aagcagggag tgagtattgt ccgtgactat cgagttctgg
     3961 ggaatatctg gataaatcaa cagaatcatc gattcctggc ccgaagaggt aagtatcagc
     4021 gccttcatta gagatactca catgcacttt ctgacctttt ttggcataga aggtatatgt
     4081 atcgtaatcg tatcccttta tttcgccgga gtactgagcg ctgctatggc cttttctgaa
     4141 ctcaacattg acattcttac ccgcagcaaa gctcgtggag gttagcagtg caagcagcaa
     4201 tacagcctta ctgatgctct tgattttcat tggtagtctc ctgttcagaa tgagtacgat
     4261 gaactgttat aatataacaa tcccttacgg gagaattcca gtggctttcc tgcgttcggt
     4321 aaaatcccgt cgcggcattt ttgccggggg gattacttat aaagggggca tggactcaac
     4381 aaaccgtaga aatctacagc tggctggctt gtggcgcggg tttcattgat gttagcttat
     4441 gccaaaacct ggcatcttaa agagtaaaag tatgttttgt gtgatttatc gaagcagcaa
     4501 gcgtgaccag acctatttat atgtcgaaaa aaaagacgat ttttcgcgtg ttcctgagga
     4561 actgataaaa ggttttggtc agcctcaatt agcgatgatt ctgccgctgg atgggcggaa
     4621 gaaactggtc aatgccgata ttgaaaaggt taaacaggca ttaaccgagc aaggttacta
     4681 tttgcagtta ccgccaccac ccgaagattt gctgaagcaa catctttccg tcatggggca
     4741 gaaaacagac gacactaaca aataaccgat atccggcggt ggcattatct ttgtcggcgc
     4801 gggttttcat atccacgata aggtgagggg aacgttatgt atcaacatca caactggcaa
     4861 ggtgcgctgc tggattatcc ggtgagtaaa gtagtttgtg ttggcagtaa ctatgccaaa
     4921 catattaaag agatgggcag cgcagtgccc gaagagccag tgctgtttat taaaccagaa
     4981 acggcactgt gcgatctgcg gcagccattg gcgatcccat ccgatttcgg ttcagttcat
     5041 catgaagtcg aactggcggt gttgattggc gcgacgctgc gtcaggctac ggaagagcat
     5101 gtccgcaaag ccattgccgg ttacggcgtg gcgctcgatc tgacattgcg tgatgttcag
     5161 ggaaaaatga agaaagccgg gcagccctgg gaaaaggcca aagcgtttga taactcttgt
     5221 ccgctttccg ggtttattcc cgcggcggaa ttcaccggcg acccgcaaaa tacaacgctg
     5281 ggcctgagcg taaacggcga gcaacgccag caaggtacga ctgcggacat gatccataaa
     5341 atcgttccgc tgatcgctta tatgagcaag ttttttaccc tcaaggccgg tgacgttgtg
     5401 ttgacaggca cgcctgatgg cgtcggcccg ttgcaaagcg gtgatgagct gacagtcact
     5461 ttcgatgggc attctttgac aactcgcgtt ttgtaatact ttttgccgcc tgaaagcggc
     5521 ggcaaaactt gcatcgctgt gccagactgg ttataaggtg cgttttaacg taatggcgga
     5581 acacctgatg agcgatgtac ctttctggca aagtaaaacc ctggacgaaa tgagcgatgc
     5641 ggaatgggag tcgttgtgtg atggttgcgg tcagtgttgc ctgcataaac tgatggatga
     5701 agacaccgac gaaatctact tcactaacgt cgcctgtcgc cagctcaata ttaaaacctg
     5761 tcaatgtcgg aactacgaac gtcgtttcga gtttgaaccc gactgcatta aattaacccg
     5821 tgaaaatctg ccaacattcg aatggctgcc aatgacctgc gcttatcgtt tgctggcgga
     5881 aggtaaagat ttacctgcgt ggcatccgct gcttactggt tcgaaagcgg caatgcatgg
     5941 tgaacgtatc tctgtgcgtc atatcgcagt gaaagaatca gaagtgattg actggcagga
     6001 tcatatctta aataaacctg actgggcgca gtgataaatt taatagatgt tgatggggct
     6061 ttcttaatat acgtctggat gctcgccatt tcgtagccct gtttttgaaa gcgctctgtc
     6121 ataatgagag ttacccgata ccttattatc aacaggtaac tctcaatctt taaaaaacgt
     6181 ctgtgttttt aaatgtgata aaccttatac cttgagtaca tggagagaat aatcgcttat
     6241 cagacttcag gtacctcaaa gagtgtcttt ttaccgtgtc ttttctgata ctcattacag
     6301 gtgttaatca tttttttggc cgcttctttt agcaaagaaa gcattaaatc atcataatca
     6361 acatagaatc tggttgtttc agtttccgtt tttatctccc cgatggcggc tatttcggtg
     6421 gttaatttca atttggcggc atcaatatct ttattcgctt gtttaaccgt gttagacagg
     6481 gtggtaaaga aactctgcac agattttaac ttgtccttca attctggaat cagtttcccc
     6541 tcaactacgc ccgcagcaat agaataggaa atgattaatc caaatggacc ggcgacgaca
     6601 ccggctgcgg caccggcata cgcttccttc ctgattttat ctacctgtga ctggaaatag
     6661 ctgctttttt ctgaaaaatc attggttaac tggctatcta acgccagcag tttcccagaa
     6721 gcgttgttga aactttgtga gcttaccagc agggattttt gcgcttcatt cagcttcgtg
     6781 atgccgtcat ccagtacctt aatgagaatg tctttctggg cggatgcttt cttctcattg
     6841 tactcatcaa atagcaaaat atacgctgcg agcaattgcg tcgcaacacc acaccattca
     6901 tacactgttt gggttgcttc aaaatactta tcctggctat ccataagtaa ggttttaata
     6961 tcgccaacta aaacggaggc tgcctgtgaa tactcctgtt taaagcgact taactctttt
     7021 atggtttcat caaaggtctg ccaggggatg acctgatcga gatattta
//
LOCUS       DAPGOF010000220         7043 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.221, whole genome shotgun sequence.
ACCESSION   DAPGOF010000220 DAPGOF010000000
VERSION     DAPGOF010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7043
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.221"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(57..920)
                     /locus_tag="RHV13_004280"
     CDS             complement(57..920)
                     /locus_tag="RHV13_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HDV1243739.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNHCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLCHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAAFIKGVGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADAFRERG
                     VSRLIFPALYL"
     gene            complement(892..2442)
                     /locus_tag="RHV13_004281"
     CDS             complement(892..2442)
                     /locus_tag="RHV13_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HDV1243740.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVIILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            2700..3479
                     /gene="deoC"
                     /locus_tag="RHV13_004282"
     CDS             2700..3479
                     /gene="deoC"
                     /locus_tag="RHV13_004282"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDV1243741.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            3606..4928
                     /gene="deoA"
                     /locus_tag="RHV13_004283"
     CDS             3606..4928
                     /gene="deoA"
                     /locus_tag="RHV13_004283"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HDV1243742.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            4980..6203
                     /gene="deoB"
                     /locus_tag="RHV13_004284"
     CDS             4980..6203
                     /gene="deoB"
                     /locus_tag="RHV13_004284"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDV1243743.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            6260..6979
                     /gene="deoD"
                     /locus_tag="RHV13_004285"
     CDS             6260..6979
                     /gene="deoD"
                     /locus_tag="RHV13_004285"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDV1243744.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
BASE COUNT         1649 a         1854 c         1925 g         1615 t
ORIGIN      
        1 gacgcgtctt atcaggccta caagtctgtg ccgaaccgta ggccgtatcc ggcatgtcac
       61 aaatacagcg ccggaaatat taaccggctc accccgcgct cccggaacgc atcagccaac
      121 ggctcaacgt cttccggcgt ggcgctcgcc cagctttgcg cctcgccata cacgccgtgg
      181 gcatgaaacg cgttcaggcg taccggaaca tcgccgactc ccttgataaa cgccgccagt
      241 tcttcgatgt gttgcaaata atccacctgg ccagggatca ccagcaaacg cagttccgcc
      301 agcttgccgc gctctgccag caaacagatg ctgcgcttaa tctgctgatt atcgcgtccg
      361 gttaattgtt gatgacattc gctcccccac gctttgagat cgagcattgc gccgtcgcac
      421 accgggagca atttttccca gccggtttcg ctcaacatac cgttactgtc cacgaggcag
      481 gtgagatggc acagttgcgg atcgtttttg atggcagtaa acagcgccac cacaaacggc
      541 agctgggtcg tggcttcacc gccactcacc gttatccctt cgataaacag cactgctttg
      601 cggacatggc taagcacttc gtccacgctc atggattgcg ccattggtgt ggcatgttgc
      661 ggacacatct tcaggcaggt atcacactgc tcgcaaacca cagcgttcca caccactttg
      721 ccgtcaacaa tctgcaacgc ctgatgcgga cactgtggca cgcactctcc gcaatgatta
      781 caacgcccca tcgtccacgg attgtgacag tttttgcagc gcagattgca gccctgcaaa
      841 aacagagcca gacgactgcc tggcccgtca acgcaggaga aggggataat cttactgact
      901 aaagcgcatc tgctgttcat ggcttatcac gcgcggctgg cgttccagaa tacgagtgtt
      961 gcgtgcggct tcttcgccca gccaggtggt gttggtgcgt gaaccttcgg cgcgatattt
     1021 ttctaaatcc gacaaacgca ccatataacc ggtaacgcga accagatcgt taccgctgac
     1081 attggcggta aattcacgca ttccggcttt aaaggcaccg aggcaaagct gtaccagtgc
     1141 ctgcgggtta cgtttgatgg tttcgtcgag cgtcagaatg tcgctgatgc cggaataata
     1201 ataagcatga tgcggggcga cagtttgcag atgggtgatc ggatctggct catcgccata
     1261 cggcaaacgc gcgcccggcg tggtgccgat atcggaactg atccccgact gtgcgtgtaa
     1321 catggcgcgt ttttgccagc catatttcac gggggtattg gcgacaaact ccgccagttg
     1381 cgcgctgatg cgataaccca cttcatttgc ggcggcttct ttaccgtagc gcgcggcaat
     1441 cccttctttc tcacacagca agttaaccgc ttccgccagc ccatacatgc caaacattgg
     1501 cacaaaacgt tcagggttaa tcagcccttc tttcaccagg aagctattct caaagaagtg
     1561 tgattgttga tagaggaatt cacaccgcgc atcgatgatg gcgatctgct gctggcagta
     1621 gtgcggtaga gtgcgcgtaa agaagtcatc cagcgattcg ctgcgctcgg caatggcttt
     1681 caggttaagg cgtaccagcg tgctgccacc acccgccagc ggcagtgagt tgtaacagct
     1741 cacaatcccg tagccccctt ttgtgaaaat tttatcatgc accggaccgt tggcgatgtg
     1801 cggtttgcta cattcacaga tgttcttcgc cacttccagc agcaggtcat caggggtgat
     1861 ttcaggatcg tagataaagg tcaggttcgg tgaaacctgc ttcaactctg cgtctgcacg
     1921 taagatcgcg cgggtaatgg gcgaatcaga cgggccgata ttggcgtgca taaaggcgtc
     1981 tggcagggtt ctgtcgaggt aacgccagaa acgttttatt cgaacatcga tctcgtcttg
     2041 tgttagaatt ataacatacg gttgcaacaa cgcatccagt tgccccaggt agaccggcat
     2101 cgatgtgacc gacggtacgt ggtggtaaag aatggtcagc agagagagtg cgtcatcaag
     2161 atctttcgcg ccttccagct ccagccattc ggaaccgttc gccagaaaac gggcgtaatc
     2221 gggtaagaca tagcgcggtt tgtacggcgc atgaccttca aacatatcgc agattacacc
     2281 ttcatccagc gcgcggcggg cttcggcagg aagctgtggg taaggcagat tgttttctgc
     2341 ttccagtgcc agaaaatggc gcttctgctc cgggctaagc actgggctgg tgacaatttg
     2401 ctggcaacgt tgttgcagtg cattttcatg agaagtgggc atcttctttt ccttttatgc
     2461 cgaaggtgat gcgccattgt aagaagtttc gtgatgttca ctttgatcct gatgcgtttg
     2521 ccaccactga cgcattcatt tgaaagtgaa ttatttgaac cagatcgcat tacagtgatg
     2581 caaacttgta agtagatttc cttaattgtg atgtgtatcg aagtgtgttg cggagtagat
     2641 gttagaatac taacaaactc gcaaggtgaa ttttattggc gacaagccag gagaatgaaa
     2701 tgactgatct gaaagcaagc agcctgcgtg cactgaaatt gatggacctg accaccctga
     2761 atgacgacga caccgacgag aaagtaattg ctctgtgtca tcaggccaaa accccggtcg
     2821 gcaataccgc cgctatctgt atctatcctc gctttatccc gattgctcgc aaaacactga
     2881 aagagcaggg caccccggaa atccgtattg ctacggtaac caacttccca cacggtaacg
     2941 acgacatcga aatcgcgctg gcagaaaccc gtgcggcaat cgcctacggt gctgatgaag
     3001 ttgacgtggt gttcccgtac cgcgcgctga tggcgggtaa cgagcaggtt ggttttgacc
     3061 tggtgaaagc ctgtaaagag gcttgcgcgg cagcgaatgt actgctgaaa gtgatcatcg
     3121 aaactggcga actgaaagac gaagcgctga tccgtaaagc gtctgaaatc tccatcaaag
     3181 cgggtgcgga cttcatcaaa acctctaccg gtaaagtggc tgtgaacgcg acgccggaaa
     3241 gcgcgcgcat catgatggaa gtgatccgtg atatgggcgt agaaaaaacc gttggtttca
     3301 aaccagcggg cggcgtgcgt actgcggaag atgcgcagaa atatctcgcc atcgcagatg
     3361 aactgttcgg tgctgactgg gcagatgcgc gtcactaccg ctttggtgct tccagcctgc
     3421 tggcaagcct gttgaaagcg ctgggccacg gtgacggtaa gagcgccagc agctactaag
     3481 taagatgctt tacgcctgat gcgctgcgct tatcaggcct acgagacgta tctacccgta
     3541 ggccggataa ggcgtagacg catccggcaa aagccgcctc atactctttt cctcgggagg
     3601 ttaccttgtt tctcgcacaa gaaattattc gtaaaaaacg tgatggtcat gcgctgagcg
     3661 atgaagaaat tcgtttcttt atcaacggta ttcgcgacaa cactatctcc gaagggcaga
     3721 ttgccgccct cgcgatgacc attttcttcc acgatatgac aatgcctgag cgtgtctcgc
     3781 tgaccatggc gatgcgagat tcaggaaccg ttctcgactg gaaaagcctg catctgaatg
     3841 gcccgattgt tgataaacac tccaccggtg gcgtcggcga tgtgacttcg ctgatgttgg
     3901 ggccgatggt cgcagcctgc ggcggctata ttccgatgat ctccggtcgc ggcctcggtc
     3961 atactggcgg tacgctcgac aaactggaat ccatccctgg cttcgacatt ttcccggatg
     4021 acaaccgttt ccgcgaaatt attaaagacg tcggcgtggc gattatcggt cagaccagtt
     4081 cactggctcc ggctgataaa cgtttctacg cgacccgtga tattaccgca accgtggact
     4141 ccatcccgct gatcactgcc tcgatcctgg cgaagaaact ggcggaaggt ctggatgcgc
     4201 tggtgatgga cgtgaaagtg ggtagcggcg cgtttatgcc gacctacgaa ctctctgaag
     4261 cccttgccga agcgattgtt ggcgtggcta acggcgctgg cgtgcgcacc accgcgctgc
     4321 tcaccgacat gaatcaggta ctggcctcca gtgcaggtaa cgcggttgaa gttcgtgaag
     4381 cggtgcagtt cctgacgggt gaatatcgta acccgcgtct gtttgatgtc acgatggcgc
     4441 tgtgtgtgga gatgctgatc tccggcaaac tggcgaaaga tgacgccgaa gcgcgcgcga
     4501 aattgcaggc ggtgctggac aacggtaaag cggcagaagt ctttggtcgt atggtagcgg
     4561 cacaaaaagg cccgactgac ttcgttgaga actacgcgaa gtatctgccg acagcgatgc
     4621 tgacgaaagc agtctatgct gataccgaag gttttgtcag tgaaatggat acccgcgcgc
     4681 tggggatggc agtggttgca atgggcggtg gacgccgtca ggcatctgac accatcgatt
     4741 acagcgtcgg ctttactgat atggcacgtc tgggcgacca ggtagacggt cagcgtccgc
     4801 tggcggttat ccacgcgaaa gacgaaaaca gctggcagga agcggcgaaa gcggtgaaag
     4861 cggcaattaa acttgccgat aaagcaccgg aaagcacacc aactgtctat cgccgtatca
     4921 gcgaataacg gtatactgat ctgatcattt aaatttgaag cactgagtac ggagaacata
     4981 tgaaacgtgc atttattatg gtgctggact cattcggcat cggcgctaca gaagatgcag
     5041 aacgctttgg tgacgtcggg gctgacaccc tgggtcatat cgcagaagct tgtgccaaag
     5101 gcgaagctga taacggtcgt aaaggcccgc tcaatctgcc aaatctgacc cgtctggggc
     5161 tggcgaaagc acacgaaggt tctaccggtt tcattccggc gggaatggac ggcaacgctg
     5221 aagttatcgg cgcgtacgca tgggcgcacg aaatgtcatc cggtaaagat accccgtctg
     5281 gtcactggga aatcgccggt gtcccggttc tgtttgagtg gggatacttc tccgatcacg
     5341 aaaacagctt cccgcaagag ctgctggata aactggtcga acgcgctaat ctgccgggtt
     5401 acctcggtaa ctgccactct tccggtacgg tcattctgga tcaattgggc gaagagcaca
     5461 tgaaaaccgg caagccgatt ttctatacct ccgctgactc tgtgttccag attgcctgcc
     5521 atgaagaaac tttcggtctg gataaactct acgaactgtg cgaaatcgcc cgtgaagagc
     5581 tgaccaacgg cggctacaac atcggtcgtg ttatcgctcg tccgtttatc ggtgacaaag
     5641 ccggtaactt ccagcgtacc ggtaaccgtc acgacctggc tgttgagccg ccagcaccga
     5701 ccgtgctgca gaaactggtt gatgaaaaac acggccaggt ggtttctgtc ggtaaaattg
     5761 cagacatcta cgccaactgc ggtatcacca aaaaagtgaa agcgacgggc ctggacgcgc
     5821 tgtttgacgc caccatcaaa gagatgaaag aagcgggtga taacaccatc gtcttcacca
     5881 actttgttga cttcgactct tcttggggcc accgtcgcga cgtcgccggt tatgctgcgg
     5941 gtctggaact gttcgaccgc cgtctgccgg agctgatgtc tctgctgcgc gatgacgaca
     6001 tcctgatcct caccgctgac cacggttgcg atccgacctg gaccggtact gaccacacgc
     6061 gtgaacacat tccggtactg gtttacggcc cgaaagtaaa accgggctca ctgggtcacc
     6121 gtgaaacctt cgcggatatc ggccagactc tggcaaaata ttttggtact tctgatatgg
     6181 aatatggcaa agccatgttc tgatggattt gggcggagcg ttgactccgc ctttgttatg
     6241 tcacaaaaag gataaaacaa tggctacccc acacattaat gcagaaatgg gcgatttcgc
     6301 tgacgtagtt ttgatgccag gcgacccgct gcgtgcgaag tatattgctg aaactttcct
     6361 tgaagatgcc cgtgaagtga acaacgttcg cggtatgctg ggcttcactg gtacttacaa
     6421 aggccgcaaa atttccgtaa tgggtcacgg tatgggtatc ccgtcctgct ccatctacac
     6481 caaagaactg atcaccgatt tcggcgtgaa gaaaattatc cgcgttggtt cctgtggcgc
     6541 agttctgccg cacgtaaaac tgcgcgacgt cgttatcggt atgggtgcct gcaccgattc
     6601 caaagttaac cgcatccgtt ttaaagacca tgactttgcc gctatcgctg actttgacat
     6661 ggtgcgtaac gcagtagatg cagctaaagc actgggcgtt gatgctcgcg tgggtaacct
     6721 gttctccgct gacctgttct actctccgga cggcgaaatg ttcgacgtga tggaaaaata
     6781 cggcatcctc ggcgtggaaa tggaagcggc tggtatctac ggcgtggctg cagagtttgg
     6841 cgcgaaagcc ctgaccatct gcaccgtgtc tgaccacatc cgcactcacg agcaaaccac
     6901 tgccgctgag cgtcagacca ccttcaacga catgatcaaa atcgcactgg aatccgttct
     6961 gctgggcgat aaagagtaat tgtgtttcgc tgaaaggcga ttgtcgtgtg aagccggagt
     7021 gggagactgc tccggctttt tag
//
LOCUS       DAPGOF010000221         7042 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.222, whole genome shotgun sequence.
ACCESSION   DAPGOF010000221 DAPGOF010000000
VERSION     DAPGOF010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7042
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.222"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            559..1548
                     /gene="araF"
                     /locus_tag="RHV13_004286"
     CDS             559..1548
                     /gene="araF"
                     /locus_tag="RHV13_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HDV1243745.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1618..3132
                     /gene="araG"
                     /locus_tag="RHV13_004287"
     CDS             1618..3132
                     /gene="araG"
                     /locus_tag="RHV13_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HDV1243746.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIN
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3147..4133
                     /gene="araH"
                     /locus_tag="RHV13_004288"
     CDS             3147..4133
                     /gene="araH"
                     /locus_tag="RHV13_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HDV1243747.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4300..5100
                     /gene="otsB"
                     /locus_tag="RHV13_004289"
     CDS             4300..5100
                     /gene="otsB"
                     /locus_tag="RHV13_004289"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HDV1243748.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLASASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELETKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            5075..6499
                     /gene="otsA"
                     /locus_tag="RHV13_004290"
     CDS             5075..6499
                     /gene="otsA"
                     /locus_tag="RHV13_004290"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HDV1243749.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6506..6934)
                     /gene="uspC"
                     /locus_tag="RHV13_004291"
     CDS             complement(6506..6934)
                     /gene="uspC"
                     /locus_tag="RHV13_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HDV1243750.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT         1773 a         1633 c         1829 g         1807 t
ORIGIN      
        1 agtgcttttt gtatcttgtt gatattaatc gatgaatttg aattcatgat gaagtgatga
       61 ttttataaaa cgttttttca tttttgcgag atggctctca ttatacgtgt cctgttaatt
      121 aatgagcaca gtgataatta atggcgaaat gaatgacgcg cattttccac atctttgagt
      181 tgtggttatt acaccatttc aaaaactcaa cgccaggtaa tgcggcctat tgactggtta
      241 aaaagaagac atcccgcatg ggtaccaaag acaacaagga tttccaggct aatcttatgg
      301 attaatctgc tgtgcattcg acaatttgtc tgacaaattg gctttccctt atgtcttttc
      361 ccgctaaatt tatgcacgtt ctcactgtaa ttctgcgatg tgatattgct ctcctatgga
      421 gaattaattt ctcgctaaaa ctatgtcaac acggtcactt atcttttagt taaaaggtaa
      481 tgctttgttt tccgattaat ttaacgaatg tcattcgttt ttgccctaca caaaacgaca
      541 ctaaagctgg agagaaccat gcacaaattt actaaagccc tggcagccat tggtctggca
      601 gccgttatgt cacaatccgc tatggcggag aacctgaagc tcggttttct ggtgaagcaa
      661 ccggaagagc cgtggttcca gaccgaatgg aagtttgccg ataaagccgg gaaggattta
      721 gggtttgagg ttattaagat tgccgtgccg gatggcgaaa aaacattgaa cgcgatcgac
      781 agcctggctg ccagtggcgc aaaaggtttc gttatttgta ctccggaccc caaactcggc
      841 tctgccatcg tcgcgaaagc gcgtggctac gatatgaaag tcattgccgt ggatgaccag
      901 tttgttaacg ccaaaggtaa gccaatggat accgttccgc tggtgatgat ggcggcgact
      961 aaaattggcg aacgtcaggg ccaggaactg tataaagaga tgcagaaacg gggttgggat
     1021 gtcaaagaaa gcgcggtgat ggcgattacc gccaacgaac tggatacggc tcgtcgccgt
     1081 actacggggt ctatggatgc gctgaaagct gcaggattcc cggaaaaaca aatttatcag
     1141 gtacctacca aatctaacga catcccgggg gcatttgacg ctgccaactc aatgctggtt
     1201 caacatccgg aagttaaaca ttggctgatc gtcggtatga acgacagcac cgtacttggc
     1261 ggcgtacgtg caaccgaagg tcagggcttt aaagcagccg atatcatcgg tattggtatt
     1321 aacggtgtgg atgccgtaag cgaactgtct aaagcacagg caaccggctt ctacggttcc
     1381 ctgctgccaa gcccggatgt tcatggctat aaatccagcg aaatgcttta caactgggta
     1441 gcaaaagacg ttgaaccgcc aaaatttacc gaagttaccg acgtggtact gatcacgcgt
     1501 gacaacttta aagaagaact ggagaaaaaa ggtttaggcg gtaagtaatt tgccggaaaa
     1561 attcccctct gcatgatgca gagggggtgt gaacgaccag tgattcacgg agacgttatg
     1621 caacagtcta ccccgtatct ctcatttcgc ggcatcggta aaacgtttcc cggcgttaag
     1681 gcgctgacgg atattagttt tgactgctat gccggtcagg ttcatgcgtt gatgggtgaa
     1741 aatggcgcag gaaaatcaac tctcttaaaa atcctcagcg gcaactatgc gccaaccacg
     1801 ggttctgtag tgattaatgg gcaggaaatg tccttttccg acacgaccgc agcacttaac
     1861 gcgggtgtgg cgattattta ccaggaactg catctcgtgc cggaaatgac cgtcgcggaa
     1921 aacatctatc tcggccagct gccgcataaa ggcggcattg tgaatcgctc attgctgaat
     1981 tatgaggcgg gtttacaact taaacatctt ggtatggata ttgacccgga cacgccgctg
     2041 aaatatctct ccattggtca gtggcagatg gttgaaattg ccaaagccct ggcgcgtaat
     2101 gccaaaatta tcgcctttga tgagccaacc agctccctct ctgcccgtga aatcgacaat
     2161 cttttccgcg ttattcgtga actgcgaaaa gaggggcggg tgatcttata cgtttctcac
     2221 cgtatggaag aaatatttgc cctcagcgat gccatcaccg tctttaaaga tgggcgttat
     2281 gtcaaaacct ttaccgatat gcagcaggtt gaccacgacg cgctggtgca ggcgatggtc
     2341 gggcgcgaca ttggcgatat ctacggctgg caaccgcgta gttatggcga ggagcgcctg
     2401 cgtcttgatg ctgtgaaagc accaggcgtg cgtacgccaa taaatctggc ggttcgcagt
     2461 ggtgaaattg ttgggctgtt tggtctggta ggggcggggc gtagcgaatt aatgaaaggc
     2521 ttgtttggcg ggacgcaaat caccgccggt caggtttata tcgaccaaca gccgatcgat
     2581 attcgtaaac cgagccacgc cattgccgca ggcatgatgc tctgcccgga agatcgcaaa
     2641 gcggaaggca ttattcctgt gcactccgtt cgcgacaata tcaacatcag tgccagacgt
     2701 aaacatgtgc tcggcggttg tgtaatcaac aacggttggg aagaaaacaa tgccgatcac
     2761 cacattcgtt cgctcaacat caaaacgccg ggcgcggagc aactgatcat gaatctctca
     2821 ggcggaaatc agcaaaaagc cattctgggc cgctggttat cggaagagat gaaggtcatt
     2881 ttgctggatg aacctacgcg cggcattgat gttggcgcta agcacgaaat atataacgta
     2941 atttatgcgc tggcggcgca gggcgtggcg gtgctgtttg cctccagcga cttacctgaa
     3001 gtcctcggcg ttgccgaccg gattgtggtg atgcgggaag gtgaaatcgc cggtgaattg
     3061 ttacacgagc aggcagatga gcgtcaggca ctgagccttg cgatgcctaa agtcagccag
     3121 gctgttgcct gagtaaggag agtatgatgt cttctgtttc tacatcgggg tctggcgcac
     3181 ctaagtcgtc attcagcttc gggcgtatct gggatcagta cggcatgctg gtggtgtttg
     3241 cggtgctttt tatcgcctgt gccatttttg tcccgaactt tgccaccttc attaatatga
     3301 aagggttggg cctggcaatt tccatgtcgg ggatggtggc gtgtggcatg ttgttctgcc
     3361 ttgcttccgg tgactttgac ctttctgtcg cctccgtaat tgcctgtgcg ggtgtcacca
     3421 cggcggtggt tatcaacctg actgaaagcc tgtggattgg cgtggcagcg gggttgctgc
     3481 ttggcattct ctgtggcctg gtcaatggct ttgttatcgc caaactgaaa ataaatgctc
     3541 tgatcacgac actggcaacg atgcagattg ttcgaggtct ggcgtacatc atttcagacg
     3601 gtaaagcggt cggtatcgaa gatgaaagct tctttgccct tggttacgct aactggttcg
     3661 gtctgcctgc gccaatctgg ctcaccgtcg cgtgtctgat tatctttggt ttgttgctga
     3721 ataaaaccac ctttggtcgt aacaccctgg cgattggcgg gaacgaagag gctgcgcgtc
     3781 tggcgggtgt accggttgtt cgcaccaaaa ttattatctt tgttctctct ggcctggtat
     3841 ctgcgatagc cggaattatt ctggcttcac gtatgactag tgggcagcca atgacgtcga
     3901 ttggttatga gcttattgtt atctccgcct gcgttttagg tggcgtttct ctgaaaggtg
     3961 gcatcggaaa aatctcatat gtggtggcgg gtatcttaat tttaggcacc gtggaaaacg
     4021 ccatgaacct gcttaatatt tctcctttcg cgcagtacgt ggttcgcggc ttaatcctgc
     4081 tggcagcggt gatcttcgac cgttacaagc aaaaagcgaa acgcactgtc tgatgctttt
     4141 ttccacaaca atttaacgtt ttttcccacc acagccagcc gccacaacgg ttggctgttc
     4201 ttcgttgcaa atggcgaccc ccgtcacact gtctatactt acatgtctgt aaagcgcgtt
     4261 ctgcgcaaaa caataagaaa agagaaggag gagaaccggg tgacagaacc gttaaccgaa
     4321 acccctgaac tatccgcgaa atatgcctgg ttttttgatc tcgatggaac gctggcggaa
     4381 atcaaaccgc atcccgatca ggtcgtcgta cctgacaata ttctgcaagg acttcagcta
     4441 ctggcaagcg caagtgatgg tgcattggca ttgatatcag ggcgctcaat ggtggagctg
     4501 gacgcactgg caaaacctta tcgcttcccg ttagcgggcg tgcatggggc ggagcgccgt
     4561 gacatcaatg gtaaaacgca tatcgttcat ctgccggatg cgattgcgcg tgatattagc
     4621 gtgcaactgc atacggtcat cgcccagtat cccggtgcgg agctggagac gaaagggatg
     4681 gcttttgcgc tgcattatcg tcaggctccg cagcatgaag acgcattaat gacattagcg
     4741 caacgtatta ctcagatctg gccacaaatg gcgttacagc agggaaagtg tgttgtcgag
     4801 atcaaaccga gaggtaccag taaaggtgag gcgattgctg cctttatgca ggaagctccc
     4861 tttatcgggc gaacgcccgt atttctgggc gatgatttaa ccgatgaatc tggcttcgca
     4921 gtcgttaacc gactgggcgg aatgtcagta aaaattggca caggcgcaac tcaggcatca
     4981 tggcgactgg cgggtgtgcc ggatgtctgg agctggcttg aaatgataac caccgcatta
     5041 caacaaaaaa gagaaaataa caggagtgat gactatgagt cgtttagtcg tagtatctaa
     5101 ccggattgca ccaccagacg agcacgccgc cagtgccggt ggccttgccg ttggcatact
     5161 gggggcactg aaagccgcag gcggactgtg gtttggctgg agtggtgaaa cagggaatga
     5221 ggatcagccg ctaaaaaagg tgaaaaaagg taacattacg tgggcctctt ttaacctcag
     5281 cgaacaggac cttgacgaat actacaacca attctccaat gccgttctct ggcccgcttt
     5341 tcattatcgg ctcgatctgg tgcaatttca gcgtcctgcc tgggacggct atctacgcgt
     5401 aaatgcgttg ctggcagata aattactgcc gctgttgcaa gacgatgaca ttatctggat
     5461 ccacgattat cacctgttgc catttgcgca tgaattacgc aaacggggag tgaataatcg
     5521 cattggtttc tttctgcata ttcctttccc gacaccggaa atcttcaacg cgctgccgac
     5581 atatgacacc ttgcttgaac agctttgtga ttatgatttg ctgggtttcc agacagaaaa
     5641 cgatcgtctg gcgttcctgg attgcctttc taacctgacc cgcgtcacga cacgtagcgc
     5701 aaaaagccat acagcctggg gcaaagcgtt tcgaacagaa gtttacccga ttggcattga
     5761 gccaaaagaa atagccaaac aggctgccgg gccactgccg ccaaaactgg cgcaacttaa
     5821 agcggaactg aaaaacgtac aaaatatctt ttctgtcgaa cggctggatt attccaaagg
     5881 tttgccagag cgttttctcg cctatgaagc gttgctggaa aaatatccgc agcatcatgg
     5941 taaaattcgt tatacccaga ttgcaccaac gtcgcgtggt gatgtgcaag cctatcagga
     6001 tattcgtcat cagctcgaaa atgaagctgg acgaattaat ggtaaatacg ggcaattagg
     6061 ctggacgccg ctttattatt tgaatcagca ttttgaccgt aaattactga tgaaaatatt
     6121 ccgctactct gacgtgggct tagtgacgcc actgcgtgac gggatgaacc tggtagcaaa
     6181 agagtatgtt gctgctcagg acccagccaa cccgggcgtt cttgttcttt cgcaatttgc
     6241 gggagcggca aacgagttaa cgtcggcgtt aattgttaac ccctacgatc gtgacgaagt
     6301 tgcagctgcg ctggatcgtg cattgactat gtcgctggcg gaacgtattt cccgtcatgc
     6361 agaaatgctg gacgttatcg tgaaaaacga tattaaccac tggcaggagt gcttcattag
     6421 cgacctaaag cagatagttc cgcgaagcgc ggaaagccag cagcgcgata aagttgctac
     6481 ctttccaaag cttgcgtagg agcggttaat ctcccgtaag tggaactaac agcacatcga
     6541 cctcacttga ggcaataact cttttcgccg agcaggatgc tcgcgaaaag aaactgtgat
     6601 tgtgattacc gcaaatcacc aaatcgaaat gatgtttgtg acatacttcc agaatatgtt
     6661 cgcttaactc accgtaggca ataaacgtct tgtcaacggg ataccccgca tcctgaatta
     6721 acttatcaag aaagctttgc gtttcttctt gcatcacgct gcgtaaatct tcaagcatcg
     6781 gcgcggctaa ttgattgtac atttccgggt cggaagcgag agtaattaaa ctgatgtgtc
     6841 cctttactgg cctggcgata gataccgctt ttgccagcag ttgctgactt tccggtgtaa
     6901 ccgcaacagc gacaagaata ttgctatagc tcataacctg ttccttattc tgtgaacttc
     6961 aggtgacatt aaagctttac tcctaatgga gaaacgacgc aatcccaact cggtcaaaca
     7021 ggtaaatatt agtctgatta tt
//
LOCUS       DAPGOF010000222         7022 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.223, whole genome shotgun sequence.
ACCESSION   DAPGOF010000222 DAPGOF010000000
VERSION     DAPGOF010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7022
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.223"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            5..340
                     /locus_tag="RHV13_004292"
                     /pseudo
     CDS             5..340
                     /locus_tag="RHV13_004292"
                     /inference="COORDINATES: protein motif:HMM:NF020698.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            326..631
                     /locus_tag="RHV13_004293"
                     /pseudo
     CDS             326..631
                     /locus_tag="RHV13_004293"
                     /inference="COORDINATES: protein motif:HMM:NF020698.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            695..922
                     /locus_tag="RHV13_004294"
                     /pseudo
     CDS             695..922
                     /locus_tag="RHV13_004294"
                     /inference="COORDINATES: protein motif:HMM:NF020698.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            986..1213
                     /locus_tag="RHV13_004295"
                     /pseudo
     CDS             986..1213
                     /locus_tag="RHV13_004295"
                     /inference="COORDINATES: protein motif:HMM:NF020698.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            1277..1396
                     /locus_tag="RHV13_004296"
                     /pseudo
     CDS             1277..1396
                     /locus_tag="RHV13_004296"
                     /inference="COORDINATES: protein motif:HMM:NF014428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            1412..5383
                     /locus_tag="RHV13_004297"
                     /pseudo
     CDS             1412..5383
                     /locus_tag="RHV13_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001614135.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            complement(5433..>7022)
                     /gene="eilA"
                     /locus_tag="RHV13_004298"
     CDS             complement(5433..>7022)
                     /gene="eilA"
                     /locus_tag="RHV13_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator EilA"
                     /protein_id="HDV1243751.1"
                     /translation="PKELGVIILLLNADGEIVSKEEIIDKVWSASVASDESLTRCIYA
                     LRKLLHENKQCKYIETVYGRGYRFTVPIVIVTDNEPVKSTTTLAVFPFRTEGSINVLK
                     LHYELVQGLSKYAFCGLDILPASVTNEASDFSTIHQFINKTGPEYYIMGQVVHYGKNW
                     RLFIELIHARTHKLIDHQSIDFNPDNPLSVLLSQVINILIEKIPNINFKSINTQQMPS
                     LDSAVMYMNGRMEMYRYTPESLKRALVIFRDCVSTQPQNTLPYCCLAECHISLALLGL
                     SEQKQAITTAVTSIEKALEINPSNSQALGLLGLISGLKDEHSVSNVLFKQAHLLKPNS
                     PDVYYYQSLLCFLNGDLARAFNLIEKSIALEPNKMGISILKLIILYYTSPLDNAISFA
                     LNLNSQNTCNNPIITSILAMFMALKGHNDKAKSLLLKLEPEHGLDYTCVNSLYTKFLI
                     YGASIKNDIMKLLANINTNKINGVILPLIYTVYGKKEYEKRWQQLIKENDLWSNVLLH
                     DPRLLSVKNEFNTIGVMRSSA"
BASE COUNT         1864 a         1580 c         1982 g         1596 t
ORIGIN      
        1 cagcatcaac aacagcagcc agagccggaa cgtgacattt atcgccgatg tcaggacggc
       61 gaagatcgct gaactggagg tcattcggga taacgcggtg gcagacggtt cgacggcgaa
      121 cacgctgcgg gcgagggtaa ccgatgcgtt cggtaatgcg cttgccgggc agacggtcag
      181 cgtgttggcg gataacggcg caacggtcgc tccgacggtc atcacagggc cggacggtac
      241 ggtggaaatc agtgtcacca gccagacggc gggcaccagt gtggtcactg ccagcatcaa
      301 caacagcagc ctgagccaga gcgtgatgtt tgtcgccgat atcaggacgg cgcagatcgc
      361 ggatctggtg gtcattaagg atggttcaga ggcggacggt tcgacggcga acacgctgcg
      421 ggcgagggtg accgatgcgt tcggcaatgc gcttgccggg cagacggtca gcgtgacgac
      481 ggacaacggc gcaacgactg ccccgacggt gagcacagag ccggacggca cggtggaaat
      541 cagtgtcacc agccagacgg cggggatcag tacagtcact gcaaccatca acaacagcac
      601 cctgagtcag aacgtgacat ttatcgccga tgtcagaacg gcgcagatcg cgagtctgga
      661 ggtgacgcag gataacgccg tggctgacgg cgcaatggcg gatatgctgc gggcgagggt
      721 caccgatgcg ttcggtaata cgcttgccgg gcagacggtc agcgtgatgg cggacaacgg
      781 tgcagcggtc gcttcgacga tgaccacaaa gccggacggc acggtggaga tcagtgtcac
      841 cagccagacg gcaggaatca gtgtggtcac cgccagcatc aacagcagca gtcagagccg
      901 ggatgtgacg tttatcgccg atatcaggac ggcgcagatc gcggacctgg tggtcattaa
      961 ggacggctca gtggctgacg gcgcaatggc gaataagctg cgggcgaggg tcaccgatgc
     1021 gttcggtaat gcacttgccg ggcagacggt cagcgtgttc gcaggcaacg gtgccacgac
     1081 tgctccgacg gtcaccacgc agccggacgg cacggtggag atctctgtca ccagccagac
     1141 ggcaggcacc agtgcggtca ctgccagcat taacagcagc agccagagcc gggatgtgac
     1201 gtttatcgcc gatgtcagaa cggcgaagat cgctgatctg gtggtcatta aggacggctc
     1261 agtggcagac ggcgcaatgg cgaacacgct gcaggtgaag gtcaccgatg cgttcggcaa
     1321 tgcgcttggc ggacagacgg tcagcgtgac ggcaggcaac agcgcaacgg tcactccgac
     1381 ggtcaccacg cagtcggacg gtacggtgga gatcagtgtc accagtcaga cggcggggat
     1441 cagtacagtc actgccagca tcaacaacag cagcctgagc cggaacgtga cgtttgtcgc
     1501 ggatgtcagg acggcgcaga tcgcggatct ggtggtcatt aaggacgact cagtggccga
     1561 cggtgcaatg gcgaatattc tgcgggcgag ggtgaccgat gcgttcggta atacgcttgc
     1621 cgggcagacg gtcagcgtgt tgtcggacaa cggtgcaacg gtcgcttcga cgatgaccac
     1681 aaagccggat ggcacggtgg agatcagtgt caccagccag acggcgggga tcagtacggt
     1741 cactgcaacc atcaacaaca gcagcctgag ccgggacgtg acatttatcg ccgatgtcag
     1801 gacggcgcag atcgcggacc tggtggtcat taaggacggc gtagtggcag acggcgcaat
     1861 ggcgaacatg ctgcgggtga gggtcaccga tgctaacggt aatacgcttg gcggacagac
     1921 ggtcagcgta tcggcagaca acagcgcaat ggtcactccg acggtgacca cgcagccgga
     1981 tggcacggtg gagatcagtg tcaccagtca gacggcgggg atcagtacgg tcactgcaac
     2041 catcaacaac agcagcctga gccggaacgt gacgtttgtc gcggatgtca ggacggcgta
     2101 gatcgttgat ctggtggtca ttaaggacga ctcagtggca gacggtgcaa tggcgaatat
     2161 gctgcgggcg agggtcaccg atgcgttcgg taatacgctt gccgggcaga cggtcagcgt
     2221 gacggcaggc aacggcgcaa cggtcactcc gacggtcacc acgcagccgg acggcacggt
     2281 ggatatcagt gtcaccagcc agacggcggg gatcagtaca gtcactgcaa ccatcaacaa
     2341 cagcaccctg agtcagaacg tgacatttat cgccgatgtc agaacggcgc agatcgcgag
     2401 tctggaggtg acgcaggata acgccgtggc tgacggcgca atggcggata tgctgcgggc
     2461 gagggtcacc gatgcgttcg gtaatgcgct tgccgggcag acggtcagcg tgatggcgga
     2521 caacggtgca gcggtcgctt cgacgatgac cacaaagccg gacggcacgg tggagatcag
     2581 tgtcaccagt cagacggcag gaatcagtgt ggtcaccgcc agcatcaaca gcagcagtca
     2641 gagccgggat gtgacgttta tcgccgatat caggacggcg cagatcgcgg acctggtggt
     2701 cattaaggac ggctcagtgg ctgacggcgc aatggcgaac atgctgcagg taaaggttac
     2761 cgatgctaac ggcaatgtgc ttgccgggca gacggtcagc atgatggcag gcaacggtgc
     2821 aacggttgcc ccgacggtga ttacagagcc ggacggtacg gtggagatcc ctgtcaccag
     2881 ccagacggcg ggagccagtg cggtcaccgc cagtatcaac agcagcaacg cgagccggaa
     2941 cgtgacgttt atcgccgatg tcagaacggc gaagatcgct gatctggtgg tcattaagga
     3001 cggctcagtg gcagacggcg caatggcgaa cacgctgcag gtgagggtca ccgatgcgtt
     3061 cggcaatgcg cttggcggac agacggtcag cgtgacggca ggcaacagcg caacggtcac
     3121 tccgacggtc accacgcagt cggacggtac ggtggagatc agtgtcacca gtcagacggc
     3181 gggagttagc gcagtcacag caaccatcaa caatcatagc ctgagccaga acgtcacctt
     3241 tgtaccgggt gacgcatcgc agctcacttc aaccgttgaa acaaataagt ctaactacac
     3301 ggttggagag accatcacca tcacggtaac gctccgggat gcgttcgata atctggtaac
     3361 cggtgcagct tcacagttag ccgctaatag tgtgctgacg gtggatggca ccgacccgtc
     3421 agaaacggga agctgggttg aatcaggtgg cgtgtatacc acaacccgaa tggcgacgat
     3481 tgccggtaac gatcagcacg ccaacctgca attgcagtcg tggagtgttg gcgtaacgtc
     3541 agatcgctat gacatacagt ccggttctcc ggcgcaagcc acatcaacta tcgccacgga
     3601 taaaaacgca tacaccgctg gcgagactat aaccgtcgcc gtgacgctga aagatgcgca
     3661 cggtaacctg gttgaagggg gcgagtcctt actgtctggt gataacgtaa cagtggaagg
     3721 agccgtacgt tcaggagagt ggtctgaaac tgccggtgtt tatactgcta catggtcggc
     3781 gcaaatggcg ggagactctc accacgcgac gctgacgttg cccgagtggg gcagcagtaa
     3841 acaatcagag agttattcca tccacagtgg tgctccggtg caggcaaatt ctgctatcag
     3901 gacagataaa tcggcatata tcgccgggga gccattaact gtcacaataa cgttgcggga
     3961 tgagtttggt aaccctgctt tagggctgac atcagaagtc atcgagtctt atatcgataa
     4021 tttcgcggta ggtggcgcaa cttccgattc tatgcagtgg gtcgagcaga ataatggtga
     4081 atataccatt gtctggacag catgggttgc cgaggaaaat ctggttgcca gtctgaaatt
     4141 gaaaacatgg gcaacggaga ttaagtcctc gttatacgga atacaaccag gtgcggcgga
     4201 aaaaactcaa tccacgattg tcgcggacaa aacgatatat attgctgggg atagcataac
     4261 ggtgaccgtt gttcttaaag atgcccagga taactttatt actgatggcg ttgttcagct
     4321 taatgaggaa aacgtacaag tcaggaatgc ggatcctatc cagggcaata attgggttta
     4381 caatggcaat ggtatatatc aaagacagta tatggcgcat tttgcggaag cgaacttgaa
     4441 tgcgcaattg aagatgactg gttggagcga tgccaattat tcaaatagct acatgattaa
     4501 accaggtgaa gtaagtatgc ttcgttcaca gcttcgtata cgcgaagttt tagtggtagc
     4561 tggggcggat atacctgtca gtgttttatt agctgatgaa tttggtaatc ctgtgaatga
     4621 cggtctggat ttgctgacag atgatgcagt gtacttacaa aatgtggaaa agaaacattg
     4681 gtccagttgg acatttgtag gtgatggcag atatgagcgt acctatatgg cttacaaaga
     4741 aggagaaaac ttaaactctt atctacatat taatggttgg tatgtagacg ggcaaccgtc
     4801 ttataccatc cttccttttg tggaagtcga gtcgcttagt gtcaatggtg caaaattcag
     4861 ggcggcagat ggttttccca aaacagggtt tgatggtgct aaatttacac tgatattaac
     4921 ccacaatatg aaaaacacgg attataattg gaccagtggc atacagggta ttcaggtcga
     4981 tagcaatggt atggtgactt tagaattcat tatcaataaa gaggttacta ttacaggaac
     5041 gccgaaaagc aataaaggga ataaagttac atataaattc tcactgcaga aatggtttat
     5101 acctcaaggg ataattcagg aatcatggag tgagatgaat tcatattgta ttggtaatgg
     5161 ttatatatta ccgtctagta cggatttagt tgggagttca actagcggtg ctgtcccgcg
     5221 aaaagtaggt tcgttatggg gtgaatatgg taatctgact tcgtatgacg gaatatttcg
     5281 cgctgaacat tattggctcg acagcggtat gatattttat ccaggagatg gtcatctctc
     5341 gattgcgtca cgttcatccc cactgtgtat gaaaacgttc tgatattatt tatgttattg
     5401 ccctgcatca gcagggcaat ttatcttcgc tcttacgcag aactccgcat cacaccaata
     5461 gtattaaatt catttttcac actgagcaaa cgggggtcat gaagcaacac atttgaccat
     5521 aaatcattct ctttaattaa ttgttgccag cgcttttcat attctttttt accatacaca
     5581 gtgtaaatta acggcaaaat aacaccattg attttgttgg tgttaatatt tgccagtaac
     5641 ttcatgatgt cattttttat agatgcgcca tagatcagaa atttcgtata aagcgagtta
     5701 acacaggtgt aatcaagacc atgttcaggt tcaagtttaa gcaataaact tttggctttg
     5761 tcattatgtc ctttcagtgc catgaacata gccagaatag atgtaataat tggattgtta
     5821 caggtgtttt gactgttaag atttaatgca aaagatatcg cgttatcgag gggggatgtg
     5881 taataaagta taattaattt aagaatgctt atgcccattt tattgggttc aagagcaatg
     5941 cttttttcta ttaaattaaa tgctcttgca aggtcgccgt tcaaaaaaca tagcagcgac
     6001 tgataataat aaacgtctgg cgaattaggt ttgagtaaat gtgcttgttt aaatagcaca
     6061 ttagacactg aatgttcatc ttttagccca ctgattaaac ccagtaaacc caatgcctga
     6121 gaatttgaag ggttaatttc cagagccttt tcaatggagg ttactgccgt agttattgct
     6181 tgtttttgtt cactgagtcc aaggagggct aatgatatat ggcattccgc caggcaacag
     6241 tagggcaagg tattttgcgg ttgcgtactc acacaatctc taaatattac taatgctcgt
     6301 ttcaaacttt ctggtgtata acggtacatt tccattctgc cattcatata cataacagca
     6361 gaatccagag atggcatttg ttgtgtatta atggatttaa aattaatgtt gggtattttt
     6421 tcaataagaa tattaattac ttgtgataat agcacggaaa gaggattgtc ggggttgaaa
     6481 tcaatacttt gatgatcaat caatttatgc gttctggcat ggattaattc aataaataaa
     6541 cgccagtttt taccatagtg aacgacctga cccataatat aatattctgg gcctgtttta
     6601 ttgataaact gatggatagt ggagaaatca ctggcttcat tcgttaccga agcgggtaaa
     6661 atatctaaac cacaaaaagc atattttgaa aggccctgga ccagttcgta atggagtttt
     6721 agaacattga tcgaaccttc agtacgaaag ggaaaaacgg caagagtggt ggtcgacttt
     6781 accggttcat tatcggttac aatcacaatg gggacagtaa aacggtatcc acgaccatag
     6841 accgtttcga tatacttaca ctgtttattc tcatgcaaaa gtttacgcaa tgcataaata
     6901 caccgtgtta aagattcatc actagcaaca ctcgcgctcc agaccttatc gataatctct
     6961 tctttgctaa caatttcacc gtcagcatta agtaatagaa tgataacgcc aagttctttg
     7021 gg
//
LOCUS       DAPGOF010000223         7016 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.224, whole genome shotgun sequence.
ACCESSION   DAPGOF010000223 DAPGOF010000000
VERSION     DAPGOF010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7016
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.224"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            331..1206
                     /locus_tag="RHV13_004299"
     CDS             331..1206
                     /locus_tag="RHV13_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HDV1243752.1"
                     /translation="MIKNNELIHPFDVTSNESGKTYQLTPNSSKSVQPVALLRLSVFT
                     PVGTKENRDRNFEVDASDELSCMEIARSEGYDDIKITGVKLSMSTDFKCWLGIIMAFS
                     KYGFTSEKITLTFNEFAKMCGISSTNINKRTRARFKESLMNLASVVLAFSDSRSGRFT
                     VTHLVQKAMIDPKSDTVELVGDPSMWELYRYDHKTLLSLQVLYILAKKEAAQSLYIYF
                     EAMPAGTLFVNMKRLRERLLLTTPIRTQNQIIRKAMRELESIGYLDYQEVKKGRDIQF
                     QIFKRSPKLALAKQG"
     gene            2355..2708
                     /locus_tag="RHV13_004300"
     CDS             2355..2708
                     /locus_tag="RHV13_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pili assembly chaperone"
                     /protein_id="HDV1243753.1"
                     /translation="MELSLNIGLSEKKSNTAALFKTKSAKVILFVLLTVLFCGLAYAG
                     SDDGALGDIWSYMSESMTGAPGKILAAAMLISSVYFSVLKPNPGLALVSLFMMLVMAN
                     GEKIISTFMDAGVPL"
     gene            2726..3076
                     /locus_tag="RHV13_004301"
     CDS             2726..3076
                     /locus_tag="RHV13_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012006609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer protein"
                     /protein_id="HDV1243754.1"
                     /translation="MRGFIPLSGFLMSKQNDVPQHTYRFPFRINMPLLILFWDAKKIG
                     LAFILVAFGNIFECFGFSVVVAVVYWIAYNKAAESGIRGLLKHRLWWLGFLPGKAVFS
                     SRYFNDPFIRDLYS"
     gene            3088..3876
                     /locus_tag="RHV13_004302"
     CDS             3088..3876
                     /locus_tag="RHV13_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HDV1243755.1"
                     /translation="MKMLSGINIPFFKKSKKDENGDLEQSYVKKDESAKGRFLDIKKR
                     FSPQAEASGAGITYSALINRDTKLIRINTVSIAVIGLLVAKILFFTDPVTIVTPPNMN
                     EEITVVGNKASESYKTQWALFFSTLLGNINPTNISFVTAYVLDALSPELQAKTSESLQ
                     EQINIMQARGVEQTFKPNDIYFDPKNDMVYVWGTKTTRLVNVPDKTESSKWTYEWVLG
                     MKNGRPRIAYVNQYSGTPNIKKITINGKEQLATLDNPPPSTGNK"
     gene            3876..5147
                     /locus_tag="RHV13_004303"
     CDS             3876..5147
                     /locus_tag="RHV13_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HDV1243756.1"
                     /translation="MIKKKGLGFNAITALIMLTTSNCVIAEEYQLPATINNPVVMPVG
                     ADGFQNGAAKAIIPGQAGSEQSGAQTNLSEAGNAQGQKPTTDLPTVQLSPASNASPAV
                     SAITGALSNNPSLPGFDAQTRSGAIDSYGRPTGTSSQQNATNATATSKADELYVEARN
                     RYKEVQRVNVPPGGNVVLPVSRGLQNRISTSFKNASVSTSTPAEEASIFVNGGDVFIS
                     TNTDKPIGIMLSEDQVPESTYNLTLVPLDVPGAMISVTTSLSPSMQAKRETSLDKQNY
                     EEMLARSQSEELAPSDPKQDDHKQRIIDLLTPVALGEVPSGFSLQQDRLSRIPASEQS
                     PCNFNMYAKLGQRLVGSRELIDVILVKNDKPYGQIVADQQCMAEGVIASALFDKAYLQ
                     PGEETELYIVRDKLFKEREARVTTRPSLIRK"
     gene            5149..5610
                     /locus_tag="RHV13_004304"
     CDS             5149..5610
                     /locus_tag="RHV13_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243757.1"
                     /translation="MGKVTLPALLGATVIGFVVFALTSFYYRSEYIPKRSVLIVPDAP
                     ISIEDGSNAFPVQTVIMNDPTLIDKFVGEDNINEENAFDYHKSNVLEPNYKHEISRTS
                     QIDKAEMNHPSVRENWARAGDPYIVPQMSDSERNLKIKRFQKPTSGANHGH"
     gene            5600..6955
                     /gene="trhB"
                     /locus_tag="RHV13_004305"
     CDS             5600..6955
                     /gene="trhB"
                     /locus_tag="RHV13_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncHI-type conjugal transfer protein TrhB"
                     /protein_id="HDV1243758.1"
                     /translation="MDIKKAWENKTVRLSVIGALLVVIVYIISQSIFSTPVKKEKKTQ
                     KKDMQTNLLLDDSQMNKLSNEESQKVYKEMVKQNRLDQNAAKEDREKAEKAQQETKAQ
                     VASLTSQLQQLSQQINDMQTNRNGNRNLDAGSSRKTINEQAPAAPYQLNANAPINGVN
                     PNYASITPTRNSPMRTITQSSIKTNGTDGVIQVMPVSENRIKEGREVIAGDKAPTRTV
                     RGDGTAPVDSKARHAARKDEMFLPATSIITGVLITGLEAPTSLSSKSEPMPVTMRIKK
                     DVIMPNNFTMDLRDCNLLGSAVGDLASQRAYIRATSISCVNSKGKAFDVKLEAYAVSE
                     NDGKNGIRGTLISRNGNAIAGAAFAGGLSSLAGSLSPSKVSSLNIDPNSQAQYQSPNF
                     GALGALAGAGAAQGGLNRLVDYYTAIAEQQWPIVEISPGRAITFVVQTGTTIPTNLTS
                     R"
BASE COUNT         2147 a         1429 c         1511 g         1929 t
ORIGIN      
        1 atttccgttt gcgatctgta gatattgtta aaggagcgct attccttgtt ttatatggac
       61 ttatcattgt tttcaacagg atagatcaat tctttagatc actaaagatc aatagagttc
      121 aagtaaagat cacggcatct ttaactcatt gattatagtg actttatgaa aggatccggc
      181 attcggatgc atgcgaaaag catttgaatg aataaggaag gcatttactg gaatgcttat
      241 ggcatttagg ttaatgcgta aaaagcattt gcaagaatat gttataaaca gtttgccaaa
      301 tgaaaatctt atcaccgacg agttaagtta atgattaaaa acaacgagtt aatccatcct
      361 tttgacgtaa ccagtaatga atcaggtaag acttatcaac tgacgcctaa ctcgtccaag
      421 tcggttcagc ccgtggccct gttgagattg agtgtattta cccctgtcgg cacaaaagaa
      481 aaccgcgacc gaaactttga agttgatgcc tctgatgagc tttcgtgtat ggaaattgca
      541 agatcggagg gttatgacga catcaagata actggtgtca aactatctat gtctaccgac
      601 tttaagtgct ggctcgggat catcatggct ttcagtaaat atggttttac ttccgagaag
      661 attaccctga cttttaacga gtttgcgaag atgtgtggta tcagttcaac aaacatcaac
      721 aaacgaactc gtgcgcgctt taaagagtca ttaatgaacc ttgcatcggt agtgcttgcc
      781 ttctcagact ctcggagtgg tcggttcaca gtaacgcatc tggtgcagaa ggctatgatt
      841 gatccaaaaa gcgatactgt tgagttggtt ggggatcctt ctatgtggga actgtatcgt
      901 tacgaccata agacattgtt aagccttcag gttctataca ttttggctaa aaaagaagct
      961 gcgcaatctc tctatattta ttttgaagca atgccagctg gtacgttgtt cgttaatatg
     1021 aaacggttga gggaaaggtt gcttcttaca acccctattc gtacgcaaaa ccagataatt
     1081 cgtaaagcaa tgcgggaact tgaatctatc ggatatctcg attatcaaga agttaagaaa
     1141 ggtcgcgaca tacaatttca gatcttcaaa agaagcccta agctggccct cgccaaacaa
     1201 ggttgaaagc taccaggtat gcactggtta taacatgctg aaaaataagt tatttatcag
     1261 tgctatgctt agtgcttctt ttactactgg tcttacatct cggcttaatg tgcaaaaaag
     1321 taacttttca aacgaactcc tgactattca tctgtatgcc tttctggctc aagcgtacat
     1381 gctagcccat tcattaaatg ccgtggagca agatggtttc gtgcgcactc tattcacgtg
     1441 aatgcctttt gtggattacg cctcttacat tcatacaaat gcttttgatg tcggtatccc
     1501 aaaaaatcat tcattgaaat gcttttgttt gccactgtat cccgagcata gctatgcagt
     1561 ggtataaaaa ggcattttgg tgaatggcat taggtcgaat gacacacagt acagctttgt
     1621 taagagcgag aatggtccaa gacatctatg acattgggca tcagaaggac tatgtgtttg
     1681 atatgagatc aaaaaaatat cagaagcatc acaactccaa tggcattcaa agaaatgcct
     1741 ttgttttgtt agtacaccaa acaacccaaa attcatgcag ttatatcaaa gactcattcg
     1801 caaaaggact cagccagaaa gcatttgctt aaatagcatt ggccaaaagc attcgaatga
     1861 atgatgttca tatggacaag acaaagtccc actcacgccc agaggtgtaa aaggcattta
     1921 gatgaatgac gcaccggccg aggattgtat gtagtttctc caactgatag ttgccgaacc
     1981 agggagcaca gtgatatgcg gccttctcag aatcaagaga tagatattaa gggaacaaaa
     2041 aagattagat atgaagctta gttacctgcc cgatatctag ttagagcttt tcaaaccatc
     2101 aaaagtgact tttatgcttg aggagtctca tcagcttcag gattgagctt aacgtaatct
     2161 taccccctcc cctctttcaa acttttacta agcgttcact ggtacttttc aaagattatg
     2221 ctttaggccc ctttgcttaa aaattcacca atcgctaaac gttaaaaaaa gggggtttat
     2281 gcgaacggaa ggatcggaat aaaaaagtat tattcattcg cgtttacgtg aactcaaatc
     2341 tacacggagt aattatggaa ctttctttaa atattggcct tagtgaaaag aaatctaaca
     2401 cggctgcact ttttaaaaca aaaagcgcaa aagtgatttt gtttgttctg ctgaccgtgc
     2461 ttttttgcgg tctggcatat gcgggttcag atgatggggc actgggagat atttggtcat
     2521 acatgagtga aagtatgact ggagctccgg gcaaaattct ggcagccgcg atgttgatct
     2581 caagcgtata tttctctgtc cttaagccta acccgggcct ggcactggtt tcattattca
     2641 tgatgctggt aatggccaac ggcgagaaaa ttatcagcac ctttatggat gctggcgtac
     2701 cgctgtaatt tattgttaat catgcatgag ggggttcatc cccctctctg gatttttaat
     2761 gagtaagcag aacgacgttc ctcagcatac ctatagattc cctttcagga tcaacatgcc
     2821 tctactgatt ttgttttggg atgcaaaaaa aatagggctt gcattcattc tggttgcatt
     2881 tgggaatatc ttcgaatgct tcgggttttc tgttgtagta gcggttgtat attggattgc
     2941 gtataacaaa gcagctgagt caggaataag aggattgcta aaacacagac tctggtggct
     3001 cggttttttg ccaggtaaag cagtttttag tagccgttat ttcaacgatc catttatcag
     3061 agatttgtat tcttgatgga gctatgaatg aaaatgctaa gtggcattaa catccctttt
     3121 ttcaaaaaat ctaaaaagga tgaaaacggt gatcttgaac agtcatacgt aaagaaagat
     3181 gaaagtgcta agggacgttt tttggacatt aaaaaacgat ttagcccaca agctgaagcg
     3241 tcaggtgctg gaattacata cagcgccctg attaatcgtg atacaaaact tatccgcatt
     3301 aatactgttt caatagccgt cattgggtta ttggttgcaa agattctttt tttcactgac
     3361 ccagtgacga ttgttacccc tccaaacatg aatgaagaga tcacggttgt tggtaacaaa
     3421 gcatcggaat cttataaaac acaatgggct ctctttttta gtaccctttt agggaatatt
     3481 aatccaacta atatttcctt tgtgacggcc tatgtcctcg atgccctttc acctgaacta
     3541 caggctaaaa cgagtgagtc tttacaggag cagataaata tcatgcaggc tcgtggtgtt
     3601 gagcagacct ttaagcctaa tgatatttac tttgatccaa aaaatgacat ggtctatgtc
     3661 tggggtacca aaacgactcg acttgtaaat gttccggaca aaactgaatc atcgaaatgg
     3721 acttatgaat gggttctcgg gatgaaaaat ggtcgcccaa gaattgcata tgtaaatcaa
     3781 tattccggaa caccgaatat taaaaaaatt acgataaacg gcaaagagca actggcaacg
     3841 ctggataatc cgccaccgtc tacaggtaac aagtgatgat taaaaaaaag ggcttagggt
     3901 tcaacgcaat taccgcattg atcatgctca cgacttcgaa ctgcgtaatc gctgaagaat
     3961 accaattgcc agcaacaatt aataacccgg ttgtaatgcc agttggtgct gacggatttc
     4021 agaatggagc tgcaaaagcc attattccgg gtcaagctgg ttctgaacag tcaggtgcac
     4081 aaacaaacct tagtgaagcc ggtaatgcgc agggacaaaa acctacaact gatctcccta
     4141 ccgtgcaact ttccccagcc agcaatgctt cgcctgcggt aagcgctatt actggagcat
     4201 tatcaaataa tcctagtctc cccggatttg acgcacagac ccgctctggt gcaattgata
     4261 gctatgggag accaacaggt acctcttccc agcagaatgc aaccaatgcg accgccacgt
     4321 caaaagccga tgaactttat gtcgaagctc gtaaccgata taaagaagtt cagcgtgtaa
     4381 atgtgccgcc tggtgggaat gtggtactgc cagtttcacg aggacttcaa aacagaatat
     4441 cgaccagctt caagaatgct tctgtcagca cttctacccc tgctgaagag gcaagcatat
     4501 ttgttaatgg tggcgatgtc tttatttcaa ctaataccga taaaccaatc gggattatgt
     4561 tgtcggaaga tcaggtacct gaatcgacct ataacctgac cctggtaccg cttgatgttc
     4621 ccggtgcgat gatttcagtt actacatctt taagcccatc tatgcaagcc aaacgtgaaa
     4681 cctcattgga taagcaaaac tatgaggaaa tgttggcccg ctcccagtca gaagaactgg
     4741 ctccatcgga tccaaaacaa gacgatcata aacaaagaat cattgattta ttgactcctg
     4801 tggctcttgg tgaggtccct tcaggattca gtttacaaca ggaccgactt tctcgtattc
     4861 ctgcgtcaga gcagtcccca tgtaacttta atatgtatgc aaagctcggt cagagactgg
     4921 ttggctcacg tgaacttatt gatgtaattc ttgtgaaaaa cgataaacca tacggtcaaa
     4981 tcgttgctga tcagcagtgt atggcagaag gcgttattgc aagtgcttta tttgataaag
     5041 catacctgca accgggagaa gaaacagagc tttacatagt ccgggataaa ttgttcaagg
     5101 aacgcgaggc ccgggttaca accagaccaa gtctaatcag aaaataatat gggaaaagtt
     5161 actttgcctg ctctgcttgg ggccaccgtg attggttttg tcgtatttgc tttaaccagc
     5221 ttttactaca ggtcagaata tataccaaaa aggagtgtat taatcgttcc ggacgcaccc
     5281 attagtatcg aagatggcag taacgctttt cccgttcaga cagtaataat gaacgatcca
     5341 actcttattg ataaatttgt aggagaggac aatattaatg aggagaatgc ttttgactat
     5401 cacaaaagta acgttttgga acctaattac aaacatgaaa tatctagaac gagccagata
     5461 gataaagctg aaatgaatca tccatctgtt cgtgaaaact gggccagagc aggtgatccc
     5521 tatatcgttc ctcagatgtc tgacagcgaa cgaaatctaa aaattaaacg attccagaaa
     5581 cctacaagtg gagctaatca tggacattaa aaaggcctgg gaaaataaga ccgtcagact
     5641 ttctgttatt ggcgctctgc tggtagtgat tgtttatatt attagccagt ctattttttc
     5701 cacaccagtt aagaaagaaa agaaaacaca gaaaaaagac atgcagacca atttgttgtt
     5761 agatgattcg caaatgaaca aattgagtaa tgaagaaagc cagaaagtat ataaggaaat
     5821 ggttaagcaa aaccgacttg atcaaaatgc ggcgaaagag gaccgcgaaa aagcagaaaa
     5881 agcccaacag gaaactaaag cccaagttgc aagtttaact tctcaacttc agcagctgtc
     5941 tcagcaaatt aatgatatgc agactaatcg aaacggcaat cgtaacttgg atgctggtag
     6001 ttcgcgtaaa acgattaatg agcaggcacc ggcagctcct tatcagctta atgctaatgc
     6061 gccgattaat ggcgtaaacc ctaattacgc gtctatcaca cctacgcgta atagcccgat
     6121 gagaacaatc acacaaagtt ccattaagac taatggtacc gatggtgtca ttcaggttat
     6181 gcccgtgtct gaaaacagaa tcaaggaagg cagagaggtc attgcaggag ataaagcgcc
     6241 tactcggact gtacgaggtg atggtactgc gccagttgat agcaaagcgc ggcatgctgc
     6301 ccgaaaagat gaaatgtttc ttccggcaac atctattatc actggtgttc tgattaccgg
     6361 gcttgaagcg cctacaagcc tttcttccaa atcagaacca atgccagtaa caatgcgtat
     6421 taaaaaagat gtcatcatgc ccaataattt caccatggat ttgcgtgact gtaaccttct
     6481 gggttcagct gttggtgatt tggcgtcaca gcgtgcttac atacgtgcca cttctatctc
     6541 ttgtgttaat tcaaaaggga aagcttttga cgtaaaactg gaagcatatg ctgtaagtga
     6601 aaatgacgga aaaaatggga tccgaggcac gcttatcagc cgaaatggta acgcaattgc
     6661 aggagctgca tttgccggtg gactttcttc acttgccggt agcttaagcc ccagtaaggt
     6721 atcttcactt aacatcgacc ctaattcaca ggctcagtat cagtccccta attttggtgc
     6781 acttggggca ttagccgggg ctggtgcagc tcaaggtggc cttaatcgac tcgtcgatta
     6841 ctacaccgca attgcagaac aacagtggcc aatcgtagaa attagccctg gccgagctat
     6901 tacatttgtc gttcagaccg gaactacaat tccaacgaat ctgaccagtc gctgaggttt
     6961 aaatggatag tttagaaaaa aaagaatcga ctgaatcagc tggacctgaa atcact
//
LOCUS       DAPGOF010000224         6963 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.225, whole genome shotgun sequence.
ACCESSION   DAPGOF010000224 DAPGOF010000000
VERSION     DAPGOF010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.225"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(238..4191)
                     /locus_tag="RHV13_004306"
     CDS             complement(238..4191)
                     /locus_tag="RHV13_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HDV1243759.1"
                     /translation="MDYNIYTLGDIDFVWSAFTGIALIFSQYTGVKEFLTTAAVVAGV
                     SLFYKTWLWLQAPTKNELPFFSWFLGLILFMMAMVRVDVTIESVKSGEVRNVDGIPIF
                     IAAMATVTTNLSQGLLKDYKTAFDPLSPVDLSATTLDDDITLGPMIRFVKFLQWGGDS
                     QGYCSAFPEPASGLGPMNVCATVQSLAYNCLKATQNSSANIAGKETIFNDIFSANLAD
                     SMDRINQAMKGALKNASASIVGANGSKSGTCDEVWSTVKQVTSTAEARQTISLIGQTN
                     GILTPDEANGAPTGASFTDVMASANGMYGKAIGSYDATLNLFIMNELRNGASKYKTPL
                     GLASDMQLFEASLKRTNTMASQGQLWLQLSGAAIAFLEMFAYMVAPFALLMLLALGGN
                     GVAAAAKYLQLILFVNMWPLTAVMVNAYVKKVATADLDTWSTLNSQNNAVTWMGLPGL
                     AETYSSYLSVASALYALIPVLTLFLMTQSIHPMMNAVKGVTPDAPVDTGHVTPKVWDG
                     PNSGKSSFGDVTRTALTSTGQGYSDGGAVDSSNFRLGMWNAGSSIANSQGQGSAVTSS
                     VMSAASNSFQAGYSQMSEIGRSGQSSQQFSTNLQTMKQISDKIGASVAEGIATKHGVS
                     ASQMASIASNVILNAGLNGGAGTGNGAGLKAAVAGQLSSGASKTNTGSDSLSNDLSKA
                     ITNQLSKDSALTDQFSKAASQVSSDQISNTNAFKEASSKMNQATQTMAQNISTSVSTN
                     ASSNSGMSLDSKQSINLDRFSDSIRNKNFSDDDVRNFARKNGLDENAFMEKFNSYNDT
                     FKASNQLGSQLQRTDALVAATRDFSEQKIAIDTARGETAESNKQDLRETSSLLKSLVS
                     DFGGNAQQLLPITNQLDRISGDGSGINTITQAQDRTPDSVNTSGVMSASRVGELGGSV
                     DSQAKLGLSSNAQDATQRVPGKSEAGFTPYNLDNAGKGDIQGIHNNNVGRTYSDEERN
                     VLNSLKTNGPVLNNQGVEKVVNSGQDVRNAEGTFNDLEKATGGRVVGDGMDQRATALN
                     SMYQSGQVRGLSNNTDNYFSRVANNPNLSRDDKRAELAQQAVFTYGASTMATGAEREQ
                     LKADTQKILNELGNYNVNWSMNDVQSIHSSFNTHNRADGSLESVVRANLGEGGSGGGL
                     VGNRTQTVTDRLVGEKIEANTERGAISGALLGGQQFVSDTLTSVGAKPVNEMLTGAGI
                     LQTQTSIANDASNPANMPDSLQGKVLNHMQMSDGVAAVSDRYQSISSDGVSTYANAAQ
                     NSERAIRQQLTDDPRFGPQKADEFIQYMKSELSNTNEPYQSRVDKADQWLNENKR"
     gene            complement(4200..5615)
                     /gene="trhH"
                     /locus_tag="RHV13_004307"
     CDS             complement(4200..5615)
                     /gene="trhH"
                     /locus_tag="RHV13_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncHI-type conjugal transfer protein TrhH"
                     /protein_id="HDV1243760.1"
                     /translation="MRSNAVFNFKKLLLSLSVCAAILAPTANADNAMRNIFNGMMTST
                     SPATFSTATRTGIVGGSMSYRTTNVNTNLVSMSFPKASVGCNGIDVFLGSFSMINGDQ
                     LVQVARGIAQGAAIYAFNVAVSAICADCAATINDIQNKLQALNKFAKDSCNATYSFLS
                     ENVGTPSQFANSVSSGPASILGSINGLIPDFGSSMTKSPEAVTSQVKAKDPEEFAEKF
                     SGNLFYMSFMDIDKGTMNIGGVTELSGYKLAEQLMSLVGTVIINWDSKGEKAGMEVRP
                     STMTVTDYIMGPPAGGSIKMLKCSPAPDPSSPRKAQCLVMSEVNDGGFKGLKDTISDL
                     LLNVQKKIINDVRVSDDELRIISYIGIPTIIDSLQTFEAPEGYAYIQDISTIAATSLV
                     INMLRQVEAKISTMSIPSESLSGKRDDLNRLTDNLSKQVKAAYELSHSQVGTSSDVIS
                     TWDNRRLQRKAFTESIRGTRN"
     gene            complement(5605..6651)
                     /locus_tag="RHV13_004308"
     CDS             complement(5605..6651)
                     /locus_tag="RHV13_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDV1243761.1"
                     /translation="MTRIALAPSKMVLLMSLVITGAHASPEQPLIKDTPFVSGQAYKK
                     GFFWYDDPAKKSEAEEEKVLPPTGAASSPSKEEKVDLNSKWLKENMPRLLTQAMDNPT
                     AENLSRYYTAQRLMLDISTRFSDKSKDYFLKNPMMSEKRRQPVEKVALDAHRTVVEKN
                     QQTVMKDIFTKSGLFFFFQSTCQFCHEESQILQFMQNYYSVDILPISMDGRPLHNGLF
                     QDFNIPNAQIIDQFKIREVPTIFLVSKDGTSAQRISEGMISADELKNTIILAAKGMNL
                     IDDASFQSTLDIKRQYTIGDDGVITVNKSEMESDPFLLQKIMDQKLEGYDMPTADPVN
                     YLNAGGSFGGTYAQ"
BASE COUNT         1817 a         1648 c         1540 g         1958 t
ORIGIN      
        1 acgacgttct ctatcattaa tagatggatg gttaatatct tttactttgt ttgccatgtc
       61 ctgtgtctca gtgcctattc agccggaaca tctactacat aaagaacgag attgtacaga
      121 aatcagctaa gtttatgaat aaaaaaggcc gcaaatttat actttgcggc cttcactgtt
      181 taacaccata ttttagtaaa tacgtttaat tttcattagg ttatatttga aactcaccta
      241 ccttttattt tcattaagcc actgatccgc tttatcgaca cgtgactggt aaggctcatt
      301 tgtgttgctc agctctgact tcatgtactg aatgaattca tctgctttct gaggcccaaa
      361 gcgtgggtca tccgtcaatt gctgccggat agccctttca gagttctgcg ctgcattcgc
      421 ataagtcgaa acgccgtcgc tgctaatact ctgataccgg tcacttacag ctgcgacgcc
      481 atcagacatc tgcatgtgat taaggacttt accctggaga gaatcaggca tatttgcagg
      541 gttagaggca tcatttgcta tcgatgtctg ggtttgaagg atgcccgctc ccgtaagcat
      601 ctcatttacc ggtttcgccc caacgctggt tagcgtatcc gaaacaaatt gctggccccc
      661 gagtagcgca ccagaaatcg cgccccgctc agtgttggcc tctatctttt ccccaaccaa
      721 gcgatctgtg acggtctgag tgcggttacc aacgagacca ccgcccgacc caccctctcc
      781 taggtttgcc cgtacgactg actcaagtga accatcagct ctgttatgag tgttgaaact
      841 gctatggatg ctctgaacgt cattcatact ccagttaacg ttatagttac ccagttcatt
      901 aaggatcttc tgggtatccg ctttgagctg ctctctttcg gcaccggtag ccatggtgct
      961 ggcaccatag gtaaagacag cttgctgtgc caattcagct cgcttatcgt cccggcttaa
     1021 gttcgggttg ttggcaacac gactaaagta gttatcagtg ttattagata agccacgaac
     1081 ttgaccgctc tgatacatac tgttcaaagc agtggctcgt tgatccattc catcaccaac
     1141 cacccgacct ccggtagcct tttccagatc gttaaatgtg ccttctgcat ttctgacatc
     1201 ctgacctgaa ttgacgactt tctctacacc ctgattattc agtacagggc cattcgtttt
     1261 gagagaattg agcacattcc tttcttcatc agaataagtg cggcccacgt tgttgttatg
     1321 tattccctga atatcgcctt tgcccgcgtt atcaaggtta tacggagtaa agccagcttc
     1381 actttttcca gggacacgct gtgtcgcgtc ctgagcgttg gaggaaagac cgagtttagc
     1441 ctgcgaatct acacttccac ccagttcccc tacgcgtgac gcactcatca cgccagacgt
     1501 atttacgctg tcaggagttc tatcctgtgc ctgagtgata gtattaattc ctgacccgtc
     1561 gcctgaaatt ctgtcgagct gattggtgat aggtagcagc tgctgtgcat taccaccgaa
     1621 gtcggaaacc agacttttaa gcaggctgga ggtttcacgg agatcctgtt tattactttc
     1681 ggccgtctct ccccgggcgg tatcaatcgc aattttttgc tcactgaaat ctcgtgttgc
     1741 agccacaagc gcgtcagtac gttgaagctg cgatccaagc tgattactgg ccttgaaggt
     1801 atcgttatag gagttgaatt tctccataaa cgcattctca tccagcccat tcttacgagc
     1861 aaaattcctt acatcatcat cactgaagtt tttgttgcga atagaatcgg agaacctgtc
     1921 caggttaatg ctttgtttag aatcaaggct cattccacta ttggagcttg cgttagtcga
     1981 aaccgatgta gaaatatttt gcgccatcgt ctgtgtcgcc tggttcattt tcgacgatgc
     2041 ttctttaaac gcattagtat tggatatctg atcgctgctt acctgagacg ccgctttact
     2101 gaactggtca gttaacgcag aatccttgct aagctgattc gtgattgctt ttgaaaggtc
     2161 atttgaaagc gagtcactgc ctgtattggt cttagatgca ccgctactca attgccctgc
     2221 tacagccgct ttcagcccgg ctccattgcc agttccagca cctccattaa gcccagcgtt
     2281 gagaataaca tttgaagcaa tgctggccat ctgagacgca ctcaccccat gtttcgttgc
     2341 aataccttct gctacagagg caccaatctt atccgagatc tgcttcattg tttgcaggtt
     2401 ggttgagaac tgctggctcg actgtcctga ccttccaatt tcgctcatct gactgtaacc
     2461 agcctggaat gaattacttg ccgcactcat aactgatgag gttacagctg atccttgccc
     2521 ctggctgtta gcgatacttg aaccagcatt ccacattcca agacggaaat tacttgaatc
     2581 aactgcgccg ccatcgctgt atccctgacc ggtactggtt aaggcggttc gtgtcacgtc
     2641 accaaatgaa gatttaccgc tgttagggcc atcccatact ttgggcgtta catgaccagt
     2701 atccaccggc gcatcggggg tcactccctt aaccgcattc atcatcgggt gaatcgactg
     2761 tgtcatcagg aataatgtga gtaccggaat cagagcatag agggcggaag caacagacag
     2821 gtatgaactg tatgtttccg caagccctgg gagtcccatc cacgtgacgg cattattctg
     2881 gctgttgagg gtgctccagg tatccaagtc ggccgtcgcg actttcttca catacgcgtt
     2941 gaccattact gccgtaagcg gccacatatt cacgaagagg ataagttgca aatatttcgc
     3001 cgcagctgca acaccattac cacctaacgc cagcagcata agcaaagcaa atggagcaac
     3061 catataagca aacatttcaa ggaatgcgat tgctgctccc gacagttgta accacaactg
     3121 gccctgtgaa gccattgtgt tggttcgctt cagagaagcc tcaaacaact gcatatcgga
     3181 agccagaccg agtggggtct tatatttgct tgcgccattt ctgagctcat tcattatgaa
     3241 caggttcagg gtggcgtcat acgaaccaat agccttaccg tacataccat ttgctgacgc
     3301 cataacatca gtaaagcttg cgccggtagg tgcaccattt gcttcgtctg gagtaaggat
     3361 gccgtttgtc tgtcctataa gggaaatagt ctggcgagct tcagctgtac tggtaacctg
     3421 tttcacagtt gaccagacct catcacacgt accagatttt gaaccgttag cgccaacaat
     3481 actggcagag gcattcttca gtgcgccttt catcgcctga ttaatccggt ccatgctatc
     3541 tgccagatta gctgagaaaa tatcgttaaa aatagtttct ttaccggcaa tatttgcgct
     3601 tgagttttgg gtagccttca ggcagttgta tgcaagagac tgaacggtag cacatacgtt
     3661 cattggccct agcccgctgg ccggttcagg aaatgcagaa cagtagccct ggctatcgcc
     3721 gccccattgc aaaaacttca cgaatcggat catcggaccg agggtgatgt catcgtccag
     3781 agttgttgca gacaggtcca ccggagaaag cggatcaaac gcagttttat aatccttcag
     3841 caaaccctgg cttaagttag tggtgacggt ggccatcgca gcaataaaaa ttggaattcc
     3901 gtcgacgttt ctgacctctc ccgactttac agattcaata gttacatcca ctcgaaccat
     3961 tgccatcatg aaaaggatca accccaaaaa ccaggagaaa aacggtaatt catttttggt
     4021 cggagcctga agccatagcc acgttttata gaataggctc acacctgcta caacggcggc
     4081 cgtggtcagg aactctttta caccagtata ttgagaaaag attagggcaa taccggtaaa
     4141 agccgaccag acaaaatcaa tatcacccag ggtatatatg ttgtaatcca tgtcagacct
     4201 cagttacgtg tgccacgaat actttcagtg aaagccttcc gttgcaggcg acggttatcc
     4261 caggttgaaa tgacgtccga gctggtaccg acctggctgt gcgatagctc ataggcggct
     4321 ttaacctgtt ttgagagatt gtctgttaat cggttgagat catccctttt acccgacagc
     4381 gactcactcg gtatgctcat ggttgaaatt ttggcctcaa cctggcgcaa catgttgatt
     4441 acgaggctcg tcgcagcaat agtgcttatg tcctgaatgt atgcgtaccc ttccggtgct
     4501 tcgaatgttt gcaaagagtc gataatggtc gggatgccga tataggagat aatgcgtaat
     4561 tcatcatctg aaacacgaac gtcgttgatg atttttttct gtacgtttaa aagaagatca
     4621 gaaatggtat ccttcaatcc cttaaaacca ccgtcattaa cttctgacat gacaaggcac
     4681 tgagcctttc gtggggatga tggatctggt gccggagaac acttgagcat tttaattgat
     4741 ccgccagctg gtggccccat gatgtaatcc gtgacagtca tggtagacgg gcggacttcc
     4801 attcccgctt tttcgccttt gctatcccag ttgataatga cagtacccac gagagacata
     4861 agttgttctg ccaatttata accggataac tccgttaccc ctccgatgtt cattgttccc
     4921 ttatcaatat ccatgaacga catatagaag aggttaccac tgaatttttc agcgaactct
     4981 tcgggatcct ttgctttaac ctgagaagtg acagcttcag gtgactttgt catcgatgag
     5041 ccaaaatcag gaatgagacc attaatcgat cccagaattg aggctggtcc agagctaact
     5101 gagttagcga actggcttgg cgtacccacg ttttcagaaa ggaaagagta agttgcattg
     5161 catgaatctt tcgcaaattt atttagcgct tgcagtttgt tttggatgtc attaatcgtt
     5221 gcagcacagt cagcacaaat agctgaaaca gcaacgttaa aagcatagat agccgcaccc
     5281 tgggcaatac cacgggccac ctgcacaagc tggtccccgt tgatcatgct gaaggaccca
     5341 agaaaaacgt caataccatt acaaccgaca gaagcttttg gaaacgacat ggaaacaagg
     5401 ttggtattaa cgtttgttgt tcggtaagac attgacccgc caactatgcc tgtacgggtt
     5461 gctgtggaaa aggtggccgg gctggtagac gtcatcatgc cattgaaaat gttacgcatt
     5521 gcgttgtctg catttgcagt cggggctaaa attgcagcac aaacagaaag agacaaaagt
     5581 aactttttga agttgaaaac ggcattactg cgcataagtg cctccaaaac tgccgccagc
     5641 attgagataa ttgaccggat cggccgtagg catgtcatag ccttcgagtt tttggtccat
     5701 tattttttga agtaagaatg ggtctgattc catttcggat ttattaacgg taataacgcc
     5761 atcatcaccg atggtatatt gccttttaat atctagagtt gactgaaacg aagcgtcatc
     5821 gatcagattc atgcccttcg cggcaagtat aatagtgttc tttaattcat cagcggagat
     5881 catgccttca ctaatgcgct gagctgatgt cccatccttt gaaaccagga aaattgtagg
     5941 tacctctcga attttaaatt gatcaataat ttgtgcattg gggatgttaa aatcctgaaa
     6001 aaggccatta tgcaatggcc ttccatccat actgattgga agaatatcta ccgaataata
     6061 gttctgcata aattgaagta tttggctctc ttcgtggcaa aactggcaag tactctggaa
     6121 aaagaaaaat aaacctgact tagtaaagat atctttcatt accgtttgct gatttttttc
     6181 aacaacagtg cggtgagcat ccagtgccac cttttccact ggttgcctgc gtttttcaga
     6241 catcatcggg tttttaagaa aataatcttt tgatttgtca gaaaaacgcg tactgatatc
     6301 cagcattaac ctttgcgccg tgtaataacg tgatagattt tctgcggtag ggttatccat
     6361 tgcctgcgtt aacagtcgag gcatattctc ttttagccat tttgaattta aatctacctt
     6421 ttcctctttt gaaggcgagc tagcagcacc ggttggtggc aaaacctttt cttcttcagc
     6481 ttcgcttttt ttagcaggat cgtcatacca gaagaaaccc ttcttataag cctggcctga
     6541 tacaaagggc gtatctttta ttaggggttg ctcagggctg gcgtgggcac cagtaattac
     6601 caaagacata agcaaaacca tctttgaagg cgctaatgca atacgagtca tagaccgttc
     6661 tccttttctg atgcggttat gatggcatac aaaaaaaagg ttagagctcg tatagcatgg
     6721 tcgtttttat gttgaaaagg acggaaaagg agtgctgact aagaagatag tctcggtgat
     6781 ggaaagatca tccatcttgc acattgtgaa aaatccggtg taacaaaaac atggttttat
     6841 cgaatatcta aaagcaaata ttgctttgaa aacaggtgta ttaagagctg aaaaaaatca
     6901 tcaccagtaa cgctgtttct atatttcaaa gaaggaatag ctaaggatca aagaagcgaa
     6961 ctt
//
LOCUS       DAPGOF010000225         6923 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.226, whole genome shotgun sequence.
ACCESSION   DAPGOF010000225 DAPGOF010000000
VERSION     DAPGOF010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.226"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            283..1815
                     /locus_tag="RHV13_004309"
     CDS             283..1815
                     /locus_tag="RHV13_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HDV1243762.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRYNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            complement(1867..2586)
                     /gene="fadR"
                     /locus_tag="RHV13_004310"
     CDS             complement(1867..2586)
                     /gene="fadR"
                     /locus_tag="RHV13_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HDV1243763.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            2808..4349
                     /gene="nhaB"
                     /locus_tag="RHV13_004311"
     CDS             2808..4349
                     /gene="nhaB"
                     /locus_tag="RHV13_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HDV1243764.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            4495..5025
                     /gene="dsbB"
                     /locus_tag="RHV13_004312"
     CDS             4495..5025
                     /gene="dsbB"
                     /locus_tag="RHV13_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HDV1243765.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5071..6339)
                     /gene="umuC"
                     /locus_tag="RHV13_004313"
     CDS             complement(5071..6339)
                     /gene="umuC"
                     /locus_tag="RHV13_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HDV1243766.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(6339..6758)
                     /locus_tag="RHV13_004314"
     CDS             complement(6339..6758)
                     /locus_tag="RHV13_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HDV1243767.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
BASE COUNT         1643 a         1761 c         1708 g         1811 t
ORIGIN      
        1 acatcttgat aactcagttg aaaatattat tcaacatacg gcttttttat ggtgagctgg
       61 ctcacatctc cctgactctg cggcaatatg gcgtctttag tatctcctgc tgaacgccct
      121 ttttatccag cataaaattc cgttcagaag cggattagtg gcactctgat aataagaaat
      181 cgcaaagaaa aaaattctcc ggcgacgcgg tttttaacag gtgttctatg cttgaaatga
      241 ggtgctccac acagagagcg ccgacaacaa tgaggtgcgc gtatggcgac gatcgattct
      301 atgaataagg acaccacacg tttgagcgat ggacccgact ggacgttcga cctgctggat
      361 gtttatctgg cagagataga ccgggtggcg aaactctacc ggctggatac ctacccgcac
      421 cagattgaag tgataacctc agaacagatg atggatgcct actccagcgt cggcatgcca
      481 attaactatc cgcactggtc attcggtaaa aagtttatcg agactgaacg gctgtataag
      541 cacggtcagc aaggactggc ctatgaaatc gtcattaact ctaacccgtg tatcgcttac
      601 ctgatggaag agaacaccat taccatgcaa gcgctggtga tggctcatgc ctgctatggg
      661 cataactctt tcttcaaaaa caattactta ttccgtagct ggaccgacgc cagttcgatt
      721 gtcgattatc tgatttttgc ccgtaaatat attaccgagt gcgaagagcg ctatggcgtt
      781 gatgaagtag aacggcttct ggactcgtgc cacgcgctga tgaactacgg cgtggaccgg
      841 tacaaacgcc cgcaaaaaat ctcgctgcaa gaagagaaag cccggcagaa aagtcgcgaa
      901 gagtatttgc aaagtcaggt caatatgctc tggcgtaccc tgccgaagcg cgaggaagag
      961 aaaacggttg ctgaagcgcg ccgttatccg tccgaaccac aagaaaacct gctctatttt
     1021 atggagaaaa atgcgccact gctggaatca tggcagcgtg aaatcctgcg tattgtgcgt
     1081 aaggtgagcc agtattttta tccgcaaaaa cagactcagg tgatgaacga aggctgggcg
     1141 actttctggc actacaccat ccttaaccat ctgtatgatg aagggaaagt aacggaacgt
     1201 tttatgctgg agtttttgca cagccacacc aatgtggtct tccagccgcc ctataacagc
     1261 ccgtggtaca gcggcatcaa cccgtatgcc ctcgggttcg ccatgttcca ggatattaaa
     1321 cggatttgtc agtcgccaac ggaagaagac aaatactggt tcccggatat cgccggttcc
     1381 gactggctgg aaacgctgca tttcgcgatg cgtgatttca aagatgagag ttttatcagc
     1441 cagttcctgt caccgaaagt gatgcgtgat ttccgcttct tcaccgtgct ggatgacgat
     1501 cggtataatt atctggagat ttccgctatt cataatgaag aaggttatcg ggagatccgt
     1561 aaccggctat cgtcgcaata taacttaagt aatctggagc cgaatattca gatctggaac
     1621 gtggatttgc gcggcgaccg ttcgctgacg ctgcgttaca ttccacataa tcgcgcaccg
     1681 ctggatcggg ggcgcaaaga agtactgaag catgtgcatc gcctgtgggg atttgatgtg
     1741 atgctggaac agcaaaacga agacggcagc gtcgagttgc tggaacgttg cccgccgaga
     1801 atgggaaatc tgtaaaaaca acaaaaaacc cctcgtttga ggggtttgtt ttttaaacgg
     1861 aagggattat cgcccctgaa tggctaaatc acccggcaga tttttctgca tccggtgcca
     1921 aatctcgcca ctctcatgcc catagcgacg cactgtttcg tacacctgat cgtgcgcgcc
     1981 ttcactgcac aacgccgaca gtttgtggta gaagcccagc gccagactgc gcgcttccgg
     2041 attggcgaaa tagtgacgac caatgcgcgt atacagtccc ttcatcccgt taaggatcag
     2101 accgtaaatc gggttgccgg aagcaaacgc caggccgcgg aatatgttgt aatccagctc
     2161 ggcaaaggca tcggcgtgat cggccacttc attagcggta gccagcactt cctgcgcttt
     2221 atcgggatgc tgacgaaacg cggtgcgaat aaaaatagtg gaaatattgg tacgcaccga
     2281 cagcaaatta tcaataagct gcggcacact ttcgtgatcc agtcgcgcca gtgtttcaag
     2341 gatatttaaa ccagaggttt cccagaaatt attcaccttc gtcggcttgc catgttgaat
     2401 ggtcaaccag ccatctcgtg ccagacgctg taacacttca cgtaacgtag tacgcgttac
     2461 gccaattaat tctgaaagtt cacgttctgc gggcaaaata gtcccgggag ggaagcggtt
     2521 attccagata ctttcaataa tgtactcttc cgcgaaaccc gccgggcttt gcgccttaat
     2581 gaccatagtg agatttccat aacacagcaa aacaaagttg gactcatcat accagagggc
     2641 taactacgct gatagcagac aaaaagaaga aaaaagggat caaggcttca ttttccgcat
     2701 ctctttcccc ttgactatca agacttgcct cactcctgcc tgaccgttaa gctttgcgtt
     2761 taaacataaa aacatcattg attatttgta aggtaaggga aaccatcatg gagatctcct
     2821 ggggccgtgc gctatggcgc aattttttgg gccagtcccc cgactggtac aaactcgccc
     2881 tcatcatttt cttaatcgta aatccgttaa ttttcctcat cagccctttc gtcgctggct
     2941 ggttgctggt agcggaattt attttcactc tggcgatggc cctgaaatgc tacccgctgc
     3001 tccccggtgg cctgttggct atcgaagcgg tattcatcgg catgaccagc gcggaacacg
     3061 tccgtgaaga ggtggcggca aatcttgaag tcttgctgtt attgatgttt atggtggcgg
     3121 gtatctattt tatgaaacag ctgttgctgt tcatatttac ccgtttgctg ttaagcattc
     3181 gctccaaaat gctgctgtcg ctctcttttt gcgtggcggc agcattcctc tccgcgttcc
     3241 tcgatgcctt aaccgtcgtg gcggtggtga tcagcgttgc agtcggtttt tatggtattt
     3301 atcatcgcgt agcctcttcc cgtaccgaag acaccgacct gcaagacgat agtcatatcg
     3361 acaagcatta caaagtggtt ctggaacagt tccgtggctt tctgcgtagc ctgatgatgc
     3421 atgccggtgt cggcaccgca ttaggcggcg taatgaccat ggtgggcgaa ccacagaacc
     3481 tgatcatcgc taaagcggct ggctggcatt ttggcgattt cttcctgcgc atgtcgccgg
     3541 tgaccgttcc ggttctgatt tgtggcctgt taacctgcct gctggtagag aagctgcgtt
     3601 ggtttggcta cggtgaaacg ctgccggaga aagtccgcga agtgctgcaa cagtttgacg
     3661 atcaaagccg ccaccagcgt acccgtcagg ataaaattcg tctgattgtc caggcgatta
     3721 ttggcgtctg gctggtgact gcgctggcgt tgcatctggc ggaagttggc ttgattggtt
     3781 tgtcagtcat tattctggca acatcattga ccggtgtcac cgatgaacat gctatcggta
     3841 aagccttcac cgaatctctg ccattcaccg cactgttgac ggtgtttttc tcggtagtcg
     3901 cggtgattat cgaccaacaa ctgttttcgc cgattattca gtttgtgttg caggcatcgg
     3961 aacatgctca gctgtcgttg ttctatattt tcaacggtct gctgtcatcc atttcggata
     4021 acgtcttcgt ggggacgatt tatatcaacg aagcgaaagc ggcaatggaa agtggcgcta
     4081 tcacgttgaa gcaatacgag ctgctggcgg tcgccattaa taccggtacc aatctgccct
     4141 ctgtcgctac gccgaacggt caggctgcgt tcctgttcct gctgacctct gcactcgcgc
     4201 cattgattcg cctctcttat ggccgcatgg tgtggatggc cttgccttac accctcgtcc
     4261 tgacactcgt cggactgctc tgcgtcgagt ttacgcttgc ccctgtaacc gaatggttta
     4321 tgcaaatggg ctggatagca acgctttgat aacaacttac cgggcatata aatgcccggt
     4381 ttgccttttc gcccgataat tgtccaattg cgtcctattt ttgctgcctc ctggtggcgc
     4441 agcgaatgaa ttggtttaaa ctgcgcactc tatgcatatt gcagggaaat gattatgttg
     4501 cgatttttga accaatgttc acaaggccgg ggcgcgtggc tgttgatggc gtttactgct
     4561 ctggcactgg aactgacggc gctgtggttc cagcatgtga tgttactgaa accttgcgtg
     4621 ctctgtattt atgaacgctg cgcattattc ggcgttctgg gtgctgcgct gattggcgcg
     4681 atcgccccaa aaacgccact gcgttatgta gcgatggtta tctggttgta tagtgcgttc
     4741 cgcggtgtgc agttaactta cgagcacacc atgcttcagc tctatccttc gccgtttgcc
     4801 acctgtgatt ttatggttcg tttcccggaa tggctgccgc tggataaatg ggtgccgcaa
     4861 gtgtttgtcg cctctggcga ttgcgccgag cgtcagtggg attttttagg tctggaaatg
     4921 ccgcagtggc tgctcggtat ttttatcgct tacctgattg tcgcagtgct ggtggtgatt
     4981 tcccagccgt ttaaagcgaa aaaacgtgac ctgttcggtc gctaatccat tcggcgctcc
     5041 tgcgggagcg cttttttcct gccgctgtgt ttatttgacc cgcagtaaat cagaacttcg
     5101 cgttgtataa cgtggtgaaa gcatggctcg cttcatctgc cattgttgct ggatcccctg
     5161 cccggcaaaa tagagtgttc ctctgccctc tttagcattc agtgtatcca ttaccgccat
     5221 caattgctca ctcccggggc gcggagcgtt atcatcgaat aaattgagct gcgcgactcc
     5281 ctgactgaag aaatccccca gcatcacgcc cgctttttgg taacgatggc ccgcttgcca
     5341 gatggcatcc agagatcgcg tagcggcgtt aatgatatcc ctgctgtcct gagtgggcgt
     5401 cagcagtttt accgacgcgc tattgccgta ataaggttca ttgagcgcaa atggtgacgt
     5461 cttaataaac gtggagataa accgacaata ttgatgctcg ctgcgaagtt tttccgccgc
     5521 ccgggcagcg taactacaaa tggcctgccg catcgacgta taatccgtga tgcgttcacc
     5581 aaacgagcgg gaacagataa tttcctgctt cgtcggtgca aactcttcca gttgcaaaca
     5641 gggttcaccg cgcagttcac gcaccgttct ttcgagcacg acattaaaat gtttacggat
     5701 aaaccggata tctgtatccg ccaaatcgag aacggttttg atccccatcg cgtccagttt
     5761 tttgctgatc cgccgtccaa tcccccagac gtcatccacg gggagagcag acattaattt
     5821 acgctggcgt tccagatttg ataaatccac caccccaccc gtctgccgct gccatttttt
     5881 tgccgcatga ttggcaagct tagccagcgt tttggtctgg gcgatcccca caccaacagt
     5941 aagatgggta cgttgtagca ccgttgcgcg aatttctctg ccaaagtcag tcagatcgcg
     6001 acaattacgc acacctgtca gatcgcagaa tgcctcatca atactgtaaa tctcgacgcg
     6061 gggcgatagt tcttccagcg tcgacatcac ccgattgctc atgtctgcgt aaagctcata
     6121 attgctgcta aagcaaacca cgccacagcg acgaaacaga tctttttgtt tgaaccaggg
     6181 atcgcccatt ttaacgccaa gcgcctttgc ctcagcgttt cgggcgataa cgcaaccgtc
     6241 attattcgat agcacaacca ccggtttacc ccataaatca gggcgaaaca ccgtctcaca
     6301 gctggcataa aacgcgttta catcacagag ggcaaacatc agcgcatcgc cttaacgacg
     6361 tggatcacca caccaaagac atccagcgta tcttcactac tgatggtaat gggcgagtac
     6421 gcgctgttca tgggaataag ctgtaccgtc gggcgtagtt gcaatttttt caccgtaaac
     6481 tcgccgtcaa cagcagcgat gacaatatca ccatggctgg cggtaatagc gctatcgaca
     6541 atcagtaaat caccgtcact aattccacca tcaatcatag aatcaccact tgctttgacg
     6601 aagtaagtcg cgctgggatg ctggatcaac agttgattca gatcgatgcg ctgttcaacg
     6661 taatctgcgg ccggtgaagg aaagccacac tgaacaagat cgctaaatag cggaaaagtc
     6721 acaatttcgc ggagatccgc aggcttgata aacaacataa taatctgcct gaagttatac
     6781 tgtttttata tacagtagtc tgttcttgcc tgcagatcaa tactgattca ggctatcaat
     6841 atttgtcgct gcatagactg ctgatttttc gttctcttat cctgtgctca cgcggccttc
     6901 tggcgacgac gctcatccag cag
//
LOCUS       DAPGOF010000226         6860 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.227, whole genome shotgun sequence.
ACCESSION   DAPGOF010000226 DAPGOF010000000
VERSION     DAPGOF010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6860
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.227"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..4541)
                     /locus_tag="RHV13_004315"
     CDS             complement(<1..4541)
                     /locus_tag="RHV13_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HDV1243768.1"
                     /translation="MSEGPGGPQGATAGGTLAMRMLSQQAMVASQMKRAVNDKAIAQM
                     LASKKSGPPAARLGDEIQHKSFLGALAGAVLGAIVTIAEGCLIMAACATGPYALVLVP
                     ALMYASYKASDYVEEKQNQLESWINSFCDTDGAINTGSENVKINGKPAARAAVTLPPP
                     PPPGAIPEVPQGEPSWGDIATDLLESAAEKAVPLAKAWGNAVITLTDSNASFMDRVSA
                     GASLLFPSGPVLMEFATMVGGRGEIKKDVDFPEAGEDTALCDKENKPPRIAQGSSNVF
                     INNQPAARQGDKLECSAAIVEGSPDVFIGGEQVTYLDIQPEFPPWQRMILGGITIASY
                     LLPPAGLLGKLGNLAKLGKLGKLGNLLGKSGKLLGANLGALLGRTKNALKSAYNVVKK
                     FIKDPVDPVTGAYCDERTDFTLGQTLPLSFTRFHCSVLPLHGLTGVGWSDSWSEYAWV
                     REQGNRVDIISLGATLNFAFDGESDTAVNPYHAQYILRRRDDYLELFDRDALSSRFFY
                     DAFPGMRLRHPVTDDTSDDRLAHSPADRMYMLGGMSDTASNRITFERDSQYRITGVSH
                     TDGIRLKLTYHASGYLKAIHRTDNGIQTLATYEQDARGRLTEADARLDYHLFYEYDAA
                     DRIIRWSDNDQTWSRFTYDAQGRCVTVTGAEGYYNATLDYGDGCTTVTDGKGIHCYYY
                     DPDGNILREAAPDGSTTTYEWDEFHHLLARHSPAGRVEKFEYNAAHGQLSRYTAADGA
                     EWQYCYDERGLLSNITDPAGQTWTQQCDERGLPVSLVSPQGEETRLAYTAQGLLSGIF
                     RQDERRLGIEYDHHNRPETLTDVMGREHHTEYSGHDLPVKMRGPGGQSVRLQWQQHHK
                     LSGLERTGTGAEGFRYDRHGNLLAWTDGNGVVWTMEYGPFDLPVARTDGEGHRWQYRY
                     DKDTLQLTEVINPQGESYLYILDNCGRVTEERDWGGVVWRYRYDADGLCTARVNGLEE
                     TILYGRDAAGRLAEVITPEGKTQYAYDKSGRLTGIFSPDGTSQRTGYDERGRVNVTTQ
                     GRRAIEYHYPDEHTVIRCILPPEDERDRHPDESLLKTTYRYNAAGELTEVILPGDETL
                     TFSRDEAGREVLRHSNRGFACEQGWNAAGQLTSQRAGLFPAEATWGGLLPSLVREYRY
                     DSAGNVSGVTSREDYGRETRREYRLDRNGQVTAVTASGTGLGYGEGDESYGYDSCGYL
                     KAQSAGWHRISEETDQYAGGHRLKQAGNTQYDYDAAGRMVSRTKHRDGYRPETERFRW
                     DSRDQLTGYCSAQGELWEYRYDASGRRTEKCCRQKAIRITYLWDGDSIAEIREYRDDK
                     LYSVRHLVFNGFELISQQFSRVRQPHPSVAPQWVTRTNHAVSDLTGRPLMLFNSEGKT
                     VWRPGQTSLWGLALSLPADTDYPDPRGERDPEADPGLLYAGQWQDAESGLCYNRFRYY
                     EPETGMYLVSDPLGLQGGEQTYRYVPNPCGYIDPLGLAICQLARWTKWGSEQSNISDV
                     LNSLGNRALKYANGDWIKS"
     gene            complement(4561..5022)
                     /locus_tag="RHV13_004316"
     CDS             complement(4561..5022)
                     /locus_tag="RHV13_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HDV1243769.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREPVSQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVVKSFTPLPPENDIQKDQPR"
     gene            complement(5050..6834)
                     /locus_tag="RHV13_004317"
     CDS             complement(5050..6834)
                     /locus_tag="RHV13_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HDV1243770.1"
                     /translation="MVSQQFLSLEFAQVLDKMAYLTIWQGDDVQRRVKGVVTWFELGE
                     NDKNQMLYSMKVHPPLWRAGLRQNFRIFQNEDIKSILGTILQENGVTEWSPLFSEPHP
                     SREFCVQYGETDYDFLCRMAAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWN
                     PNTRTEVSTLCISQFRYSAQIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEV
                     YDYPGRFKGAHGQNFARWQMEGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNRE
                     WQVVASELHGEQPQAVPGRRGAGTALENHFAVIPADRTWRPQPRLKPLVDGPQSAVVT
                     GPEGEEIFCDEHGRVRVKFNWDRYNPADQDSSCWIRVAQAWAGTGFGNLAIPRVGQEV
                     IVDFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATG
                     KEQVYIHAQKDMDTEVLNDRTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGV
                     LKGSLSEDVFQDRGTIAGSVHVDAVNNGGEGDGIQAYTAIKEILLAVEESKIALTPDG
                     IQLQVGESTVIRLSKDGITIVGGSVFIN"
BASE COUNT         1227 a         2145 c         1870 g         1618 t
ORIGIN      
        1 tctgatttta tccaatcacc attagcatat ttaagtgctc tattccctaa tgagttcaaa
       61 acatcagata tgttgctttg ctcactcccc catttcgtcc agcgggctaa ctgacatata
      121 gccagcccca aaggatcgat atacccacaa ggattcggca cataccgata agtctgctcc
      181 cctccctgca accccagcgg atcactcacc aggtacatcc cggtttccgg ctcgtagtac
      241 cggaaccggt tatagcacag ccccgattct gcatcctgcc actgtcccgc atacagcagg
      301 ccggggtccg cttccgggtc ccgctccccg cgcgggtccg ggtagtctgt gtctgcgggc
      361 aggctgagtg ccagccccca caggctggtc tgccccggcc gccagacggt tttaccttca
      421 ctgttaaaga gcatcagcgg gcggcccgtc aggtcgctca ccgcatgatt cgttcgcgtc
      481 acccactgcg gggccacgga cggatgcggc tgtcgtaccc ggctgaactg ctggcttatc
      541 agctcaaagc cgttaaacac caggtgcctt acgctgtaca gtttatcatc gcggtattcc
      601 cggatttccg caatactgtc gccgtcccac agatatgtaa tgcggatggc tttttgccgg
      661 cagcattttt ccgtccgcct gccgctggcg tcgtagcggt attcccacag ctcaccctgt
      721 gcgctgcaat acccggtcag ctggtcccgg ctgtcccacc ggaaccgctc tgtttccggg
      781 cggtagccgt cacggtgttt tgtccggctg accatccggc ctgcggcgtc atagtcatac
      841 tgcgtgtttc cggcctgttt cagccggtgg cctccggcat actggtcagt ctcttcactt
      901 atccggtgcc agcccgcaga ctgcgccttc aggtagccgc agctgtcata gccataggac
      961 tcatcgcctt cgccatagcc cagcccggtg cctgaggctg tcaccgccgt gacctggccg
     1021 ttccggtcca gccggtactc ccggcgtgtt tcccgtccgt aatcttcccg gctggtgacg
     1081 cctgacacat tacccgcgct gtcgtaacgg tactcccgta ccagtgaggg gagcagcccg
     1141 ccccatgtgg cttctgccgg gaaaagtccg gcgcgctggc tggtaagctg accggctgca
     1201 ttccagccct gttcacaggc aaaaccccgg ttactgtgcc ggagcacttc acgtcctgcc
     1261 tcatcacggc tgaacgtcag cgtctcatcc cccggcagaa tgacctccgt cagttctccg
     1321 gcggcgttat agcggtaggt ggttttcagc agggattcat cgggatgtct gtcgcgttca
     1381 tcttccggtg gcaggataca gcggatgacg gtgtgttcgt cggggtagtg gtattcaatg
     1441 gcccgtcggc cctgagtggt gacattcacc cgcccgcgtt cgtcgtagcc ggtgcgctgt
     1501 gatgtgccgt ccgggctgaa gatacccgtc agcctgccgg atttgtcata cgcatactgc
     1561 gttttacctt ccggcgtgat gacttctgcc aggcggcctg cggcgtcccg gccgtagagg
     1621 atggtttcct ccaggccgtt gaccctggcg gtacacaggc catcagcgtc ataacggtaa
     1681 cgccagacca cgccgcccca gtcacgttct tccgtcaccc ggccacagtt gtccagaata
     1741 taaagataag actcaccctg cgggttgatg acttctgtga gctgcagcgt gtctttatcg
     1801 tagcggtact gccagcggtg gccttcaccg tccgttcgcg ccaccggcaa atcgaacggg
     1861 ccgtactcca ttgtccagac aacgccgtta ccgtccgtcc aggccagcag gttgccgtgg
     1921 cggtcatagc ggaatccttc cgcgccggtg ccagtgcgct caaggccgct cagtttatgg
     1981 tgctgctgcc actgtaaccg cactgactga ccgccggggc cgcgcatctt caccggcagg
     2041 tcgtgaccgc tgtattcggt atggtgttca cggcccatca cgtcggtgag tgtttccggc
     2101 cggttgtggt ggtcgtactc tatgcccaga cgccgttcat cctggcggaa tatccccgac
     2161 agcagcccct gagcggtgta cgccagccgg gtctcttcgc cctgtggcga caccagactt
     2221 accggcaggc cgcgttcatc acactgctgc gtccacgtct gcccggcagg gtcggtgatg
     2281 ttgctgagca ggccgcgctc atcatagcag tactgccact ccgcgccgtc tgccgccgtg
     2341 taacggctta actgaccgtg tgcggcgttg tattcaaact tctccacccg cccggcaggg
     2401 gagtggcggg ccagcaggtg atggaattca tcccattcat acgtggtggt actgccgtcc
     2461 ggcgctgctt cccgcagaat attgccgtca ggatcatagt aataacagtg aatgcccttg
     2521 ccgtccgtca cggtggtgca gccgtcacca tagtccagcg tggcgttgta atagccctcc
     2581 gccccggtga cggtcacgca ccggccctgt gcatcgtagg tgaaacggct ccacgtctgg
     2641 tcgttatcgg accagcggat gatccggtcc gcagcgtcgt actcataaaa caggtggtag
     2701 tccagccgcg catccgcttc tgtcagccgc ccccgcgcat cctgttcgta ggtcgccagc
     2761 gtctgtatgc cgttatccgt gcggtgaatg gctttcaggt agccgctggc gtggtacgtc
     2821 agtttaagcc ggatcccgtc ggtgtgactg acacccgtga tgcggtactg gctgtcgcgc
     2881 tcaaacgtga tgcggttgct ggcggtgtcg ctcatcccgc ccagcatgta catccggtct
     2941 gcggggctgt gtgccaggcg gtcatcgctg gtatcgtcag tcaccgggtg gcgcagacgc
     3001 attcccggaa aggcgtcata aaagaagcgg ctgctcaggg catccctgtc gaacagctcc
     3061 agataatcat cacggcggcg cagaatgtac tgggcgtgat acgggttaac cgccgtatca
     3121 ctttcaccgt cgaaggcgaa gttcagcgtg gctcccaggc tgatgatatc cacccggttt
     3181 ccctgttcac gcacccaggc gtattcgctc caggagtcgc tccagcccac gccggtcagg
     3241 ccatgcagtg gcagtactga acagtggaag cgggtgaagg agagggggag tgtctggccc
     3301 agcgtaaagt cggtacgttc gtcgcagtac gcgccggtta ccgggtcaac cggatctttg
     3361 ataaactttt tcacgacgtt ataagcactt ttcagggcat tttttgttct gcccagcaac
     3421 gcgccgaggt ttgcgcccag cagcttcccg cttttcccca gcaggtttcc cagtttgccc
     3481 agtttgccca gtttcgccag attccccagt tttcccagca gtcctgctgg cggcagaaga
     3541 tagctggcta tcgttattcc tcccaggatc attctctgcc atggcgggaa ctccggctgg
     3601 atatccagat aggtgacctg ctcaccccca ataaagacgt ccggcgaacc ttccacgatc
     3661 gccgcactgc actccagttt gtccccctgg cgcgcggcag gctgattgtt gataaagacg
     3721 ttgctgctgc cctgggctat tctcggtggt ttgttctcct tgtcgcagag cgccgtgtcc
     3781 tcaccggctt ccgggaaatc cacgtctttt ttgatttcgc cacgcccgcc caccatcgtg
     3841 gcaaattcca ttaataccgg gccgctggga aacagaagcg atgcgcctgc actgacgcga
     3901 tccataaaac tggcattgct gtccgtcagg gtgataacag cgttccccca ggccttcgcc
     3961 agtggtactg ctttttccgc tgccgattca agcaggtcag tggcaatatc accccatgag
     4021 ggttccccct gtgggacttc aggtattgct ccaggtgggg gaggaggggg aagggtgacg
     4081 gcggccctgg cagcgggctt tccgttaatt tttacgtttt cagaaccggt attgatggcg
     4141 ccgtccgtgt cacaaaaact gtttatccat gactcaagct ggttctgttt ctcctccaca
     4201 taatcactcg ccttatagct ggcatacatc agcgcaggca ccagaaccag cgcataaggg
     4261 ccggtggcac aggcggccat aatcaggcaa ccttctgcga tggtcactat cgcccccagc
     4321 acggcccctg ccagcgcccc caaaaaactc ttgtgctgaa tttcatcgcc cagcctggcg
     4381 gcgggggggc cagacttttt tgatgccagc atctgtgcaa tggctttgtc gttgactgcc
     4441 cgtttcatct ggctggcgac catcgcctgc tgtgacagca ttcgcattgc cagcgtaccg
     4501 cctgcggtcg ctccctgtgg cccgcctggt ccttcactca tgttttatcc ctcgtttatg
     4561 tcagcgcggc tggtcctttt ggatgtcatt ctccggtggc aacggggtga agctctttac
     4621 cacctccagc agcatcgtct tttgactgtt cttcagggat aaggaggtgc tgaaagtgaa
     4681 ggtcagcagc gtatctccac gccgctggaa tacggctatc tggctaatcc gcccctgatt
     4741 cccttcatct tccggaccgt tccaggaata atccagcagc caggccgggg cctctgccag
     4801 agtgatttcc cggcaggcat gttcggtgta cccatcaagt ttgccctggt acagcgccag
     4861 cgtttcctgg tacagctctg gtgtcagggt tccctgtgaa acgggttcgc gggtaatgac
     4921 caggttgatg ccactgttat ccgtggatac caggacctgc atcgactgat cctgccattg
     4981 cgatggcagt tgaatatgac cttcattaaa agtgaactgc ataaaatact ccctgttaat
     5041 ctgagtgaat cagttgatga aaacagaacc gcccacgatg gtgatgccat ccttcgacag
     5101 cctgattacc gttgattccc cgacctgtag ctgaatgcca tccggcgtca gcgcaatttt
     5161 gctttcctcc acggccagca aaatttcctt aatcgccgta taagcctgta taccgtcgcc
     5221 ttcgccacca ttgtttacag cgtcaacatg caccgaaccg gcaatcgtgc ctctgtcctg
     5281 aaagacatcc tcagataaag accctttcag tactccggtg atcttgtggc ctttttccac
     5341 cgtaagaagg cgattacctt cctgtatcgt gaccgtctgg tcatttttta cggtttcgcg
     5401 atgatcgtgt tttacggtgg tggtacgatc gttgagtacc tcggtatcca tgtccttctg
     5461 cgcgtggatg tagacctgtt ctttccctgt cgcatcgtca aacttcagct cattaaaccc
     5521 gctgcccata tacgtcttcg aacggatggt catctgcgtc tttgtccccg gcaggctgcc
     5581 gggtgtgcgg ttttcctggt ggtaggtgcg ccccataatg atcggctggt ccggatcgcc
     5641 gttgaggaag tctactatca cctcctgacc cacacgcggt atcgccaggt tgccaaaacc
     5701 ggtgcctgcc cacgcctgtg ccacacggat ccagcacgag ctgtcctggt ctgccgggtt
     5761 ataacggtcc cagttgaatt tcacccgcac tcggccatgt tcatcacaga agatttcctc
     5821 accctccggc cccgtcacga cggcgctctg cggaccgtcc accagcggtt ttagccgcgg
     5881 ctggggtcgc catgtccggt ctgccgggat caccgcaaaa tgattctcca gcgccgtccc
     5941 tgctccccgc cttcccggca ccgcctgcgg ctgctcgccg tgcagttcac ttgccaccac
     6001 ctgccattcc cggttcaggt tcgcctgcgg atgccccgtc agcacaattc gccgtcccgg
     6061 ccatatctcc ggcgagcgac tcattccccg cgcggtttct gcattgtttc gccagccctc
     6121 catctgccag cgggcaaagt tctgcccgtg ggcgcccttg aaacgtccgg ggtagtcata
     6181 cacctcatac tgcgtgcgct ggtaatcctg gtgctgacct tcctgatcaa aacgtcctgc
     6241 ccagccgggg cgtttaaagg tgtagtcttt ggtcaccacg gaagaagggc ggatttgtgc
     6301 gctgtagcga aactggctga tgcagagggt gctcacctcg gtacgggtgt tcgggttcca
     6361 ggggatttca aaagattcgg gcagatggcg gaccgtgtcg cacagcacca ggctctggtc
     6421 ggtacttttg taagcatgct cctcataaaa gaagatgcct tcctccgccg ccatccggca
     6481 caggaaatcg taatcagtct caccgtactg gacacaaaac tcacgggaag gatgcggttc
     6541 actgaacagc ggactccact cggtcacccc gttttcctgc aatatcgtgc cgaggatgct
     6601 tttgatgtcc tcgttctgga agatacggaa gttctggcgc agaccggcac gccacagcgg
     6661 cgggtgcacc ttcatgctgt acagcatctg gtttttgtcg ttctccccca gttcaaacca
     6721 ggtcaccaca cctttcaccc ggcgctgtac atcatcaccc tgccatattg tcaggtaggc
     6781 cattttgtcc agcacctgtg caaattcaag ggagagaaac tgctggctga ccagggagag
     6841 atcgagggaa aagagtgaag
//
LOCUS       DAPGOF010000227         6846 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.228, whole genome shotgun sequence.
ACCESSION   DAPGOF010000227 DAPGOF010000000
VERSION     DAPGOF010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.228"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(69..1466)
                     /gene="ccp"
                     /locus_tag="RHV13_004318"
     CDS             complement(69..1466)
                     /gene="ccp"
                     /locus_tag="RHV13_004318"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HDV1243771.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1870..3519
                     /locus_tag="RHV13_004319"
     CDS             1870..3519
                     /locus_tag="RHV13_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HDV1243772.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFIENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(3570..4172)
                     /locus_tag="RHV13_004320"
     CDS             complement(3570..4172)
                     /locus_tag="RHV13_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDV1243773.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            4692..5591
                     /locus_tag="RHV13_004321"
     CDS             4692..5591
                     /locus_tag="RHV13_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV1243774.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLNELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYQHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            5640..6653
                     /gene="yhjD"
                     /locus_tag="RHV13_004322"
     CDS             5640..6653
                     /gene="yhjD"
                     /locus_tag="RHV13_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HDV1243775.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
BASE COUNT         1735 a         1774 c         1616 g         1721 t
ORIGIN      
        1 attaaaaata gactttatta tcgtaataaa aaatcgccca aacattcctg ttcgggcgat
       61 caaattaatt attgtttatc ctgcatatac ggcgtgtaca ccccgttcag actgtgcagg
      121 aaagctacga tatcatccac atcctcctgc ggcagctctt tgcctacctg atagcgcaac
      181 atcagtttca ccgccccgtc cagcgtcggc acgtcaccgc gatggaagta cggtgccgtt
      241 aaagcaacgt tacgtaaacc gggtactttc tgacgcaatt tatcacgctc ttctttagtc
      301 acattcatac gaccaatatc cgccgccgta atttcaccaa agttaaagtc ttttttcagc
      361 cccaacggtt cgaaggaacg tccgccgaga ataataccac catgacaagt tgcacattta
      421 ttatctttaa ataattgata gccttttttc tgttgcgccg tcagcgcatt ctcatctcca
      481 cgcaaccatt tatcaaatgg ggaatccggc gtaattaatg ttttctcaaa ttcagcaatg
      541 gcatcagtaa tattttcgcc actgaaacct tgcggataga cttcgaggaa ctgcgcttta
      601 agctgcggat ctttttccag cttagcaata atttcgtccc aggattttga cgccatttca
      661 atcgggttca acggcggtcc accagcctga tcctgcaatg ttgccgcacg accatcccag
      721 aactgctcaa cgttaaatac tgagttaaat accgtcggcg cgttaatcgg cccaactgcg
      781 ccaccaacac caattgatgt ttttctgcca tcgacgcccc ccgcattcaa cgcatggcaa
      841 tgagcgcatg aaatggtgct atcagccgat aaacggggat cgtgatacag cgcaaagccc
      901 aacgccactt tttgcgcatc ggtaggcagt ttttgcggga tgggctgcac cggttcattg
      961 cgatgctccg gagcagtatc attactggcg taatattctg cgcgctgttt tgcaatccag
     1021 gccagtattt ccgcccgctc ttcatcactc accttacccg cccagtgaag cgcggtataa
     1081 cgcgttggcg gcatagtttc atactgcatc acccattcaa tcttattcag atcgctttgc
     1141 gaaacgggtt tatcagccag cagagcagca cgcacggcct cgaggttaaa agatttatat
     1201 ccaagcttaa tgtcgtaatc catcaactgt ttcgcgccag gaatataata ataggcgggt
     1261 aattctgccg aaggcgtgtg gcaatagtcg catccttttt cgcggagaaa gcccaaaacc
     1321 ttattatttt cactgacagc agatgcctga acatcggcct gtttactgcg tttattatcg
     1381 tggtaccaca cataaccaga taaccctaaa tagcaaatcg cgacgccagc caggccgatc
     1441 gcggtaatac gtgagaccat tttcattatt cttccctcac ggtggtgagt tattgtgacc
     1501 cagaaaattt tcctgaatgc tgcaaacgtc tctgcgagac aaagccatca taaaaaacag
     1561 ggagatacac tttttgatag gaatcaatca attacatgaa tataaactat caataagata
     1621 gcctgtgcct attacccaga tcaggaatgg catatcatga ttgcggcgaa agcaggattg
     1681 aatgttctga aaatgaaaaa caacacgaaa tctgtatttt tataaattcc attgatatta
     1741 gtgcgttata ttttttgaca ggttaataaa cgtgatccac cgcacgcttt gtcgcccacc
     1801 aggcggagcg aatgactacc cttaaagaaa agccgataat tagcgacgaa tttcggaggt
     1861 tggatcctta tgctcaatca gaaaattcaa aaccctaatc cagacgaact gatgatcgaa
     1921 gtcgatctct gctatgaact ggacccgtat gaattaaaac tggatgagat gatcgaggca
     1981 gaaccggaac ccgagatgat tgaagggctg cccgcctctg atgcgctgac gcctgccgat
     2041 cgctatctcg aactgttcga gcatgttcag tcggcgaaaa ttttccccga cagtaaaacc
     2101 tttcccgact gcgcacctaa aatggacccg ctggatattt taatccgcta ccgtaaagtg
     2161 cgccgtcatc gtgattttga cttgcgcaag tttattgaaa accacttctg gctgccggaa
     2221 gtctactcca gcgagtatgt atcggacccg caaaattccc tgaaagagca tatcgaccag
     2281 ctgtggccgg tgctaacccg cgaaccacag gatcacattc cgtggtcttc tctgctggcg
     2341 ctaccgcagt catatattgt cccgggcggc cgttttagcg aaacctacta ttgggattcc
     2401 tatttcacca tgctggggct ggcggaaagt ggtcgggaag atttgctgaa atgcatggcc
     2461 gataacttcg cctggatgat cgaaaactac ggtcacatcc ccaacggcaa ccgcacctat
     2521 tatttgagcc gctcgcaacc accggttttt gcgctgatgg tggagttgtt tgaagaagat
     2581 ggtgtacgcg gtgcgcgccg ctatctcgac caccttaaaa tggaatatgc cttctggatg
     2641 gacggtgcag aatcgttgat ccctaatcag gcctatcgcc atgttgtgcg gatgccggac
     2701 ggatcgctgc tcaaccgtta ctgggacgat cgcgacacgc cgcgtgacga atcctggctt
     2761 gaggacgttg aaaccgcgaa acattctggt cgcccgccca acgaggtgta ccgcgattta
     2821 cgcgcggggg cggcctccgg ttgggattac tcttcccgtt ggctgcgtga tactggtcgt
     2881 ctggcgagca ttcgtaccac ccagttcatc cccatcgatc tgaatgcctt cctgtttaaa
     2941 ctggagagcg ccatcgccaa catctcggcg ctgaaaggcg agaaagagac agaagcgctg
     3001 ttccgccaga aggccagtgc ccgtcgcgat gcggtaaacc gttacctctg ggatgatgaa
     3061 aacggcatct accgcgatta cgactggcga cgcgaacaac tggcgctgtt ttccgctgcc
     3121 gccattgtgc cgctctatgt cggcatggcg aaccatgaac aggccgatcg tctggcaaac
     3181 gccgtacgca gccggttact gacacctggc gggattctgg caagcgagta cgaaaccggt
     3241 gaacagtggg ataaacccaa tggctgggca ccgttacaat ggatggcaat tcagggattt
     3301 aaaatgtatg gcgatgacct tctgggtgat gaaatcgcgc gcagctggct gaaaacggtg
     3361 aatcagttct atctggaaca gcacaaactg atcgagaaat accatattgc cgatggtgtt
     3421 ccccgcgaag gtggcggtgg cgagtatccg ttacaggatg ggtttggctg gactaacggt
     3481 gtggtacgcc gtttaattgg tttgtatggc gaaccataat atttttacag ccagccgcta
     3541 acttcctgct ggctgtaaaa ttatcctctt caggaggaga tatttaacat cattgccgcc
     3601 tgggtgcgat ttttcacttc cagacggcga tacagggatt ccagatgcgc ttttaccgtt
     3661 ccggtactga tattcagcgc tctgccgatc tccttatttg attcgcccgc cgctaacatg
     3721 gttaaaatct cccgctggcg ggcgcttaac gatttgagat ctttaatgtc cttttccggc
     3781 gtcgtccgcc agtctccagg cagaaacatc atccccatcg ccgcactatt taccgccaac
     3841 gcaaatgtct cgacggttga atcacgaggc acaatggcca gcacattaaa atgaataact
     3901 tcctgtaacc accgtttatt gcaatccgtc gccgtaatta acaccttaac ctcaggaaat
     3961 tgcaccacgg ttttttgcag caaccagtag caaaactcac catcctgatc gccatcgagc
     4021 ataactaagg cttcagggta actttccagc ttttgccata actcgtctgc ctgactggcc
     4081 ccctgaatac tcactcctgg aatacgctgc tgtaaactga ttttcattcc atgaataaat
     4141 attgactgcc tgtcaaacat gactatttgc ataactgaat ctccacctga atacgttaaa
     4201 aagacttaag tagtggaagg gtattacccg cgagaaaaaa taagaattcg ccatttggcg
     4261 gtggccattc tacagagatg acgcgtagaa aatagttacc gatataaata gttacagcta
     4321 aacgcctgaa attacatgtc gagggcacta tttaaaacaa ttctgaggat ttccttatat
     4381 tgatggttag tacgcatgca attaaaaatg aaattccgcg accacaagcc aaaataacaa
     4441 acggcaatga gaccaaaata agcacaaata gccaatacgt cctctattca ctttaaaggg
     4501 aatcgctgaa aaatacgctc tgtttaaggg gattcaccat tctcagaaag ctattccgcc
     4561 cttttcctgc tgagaaatcg ccacattcgt tatgacaaca ttgtgaaacc cggcattaga
     4621 tgttagaaaa aacaataaac aatgccatat gcgcgttact gtttcgctgt gaacaacaat
     4681 cggtcaaaga aatggataaa attcacgcaa tgcagttgtt catcaaagtc gcggagctgg
     4741 aaagtttttc ccgcgcggcg gatttctttg ctttgccaaa aggaagtgtt tcgcgccaga
     4801 tacaggcact ggaacatcaa cttggcaccc agcttctcca gcgcaccacg cgacgggtca
     4861 aactcacgcc agaaggcatg acctattatc aacgagcaaa agatgtgttg agtaatctca
     4921 acgaactgga cggtctgttt cagcaggatg ccaccagtat cagcggtaaa ttacgcatcg
     4981 acatcccgcc aggaatcgcg aaaagcctgt tattgccgcg cctgtcggaa tttctctatc
     5041 agcatccggg aattgagctg gaactgagta gccatgaccg tccggtagat attcttcatg
     5101 atggttttga ttgcgtgata cgcactggcg cgttaccgga agatggcgtt atcgcccgtc
     5161 ccctcggcaa actgaccatg gtcaactgtg ccagtccgca ctatctgacg cgctttggtt
     5221 atccgcaaag ccccgatgat ctgacttcac acgcaatagt gcgttataca ccgcacctgg
     5281 gtgtacatcc gttaggtttt gaggttgcca gcgttaatgg cgtccagtgg tttaagtctg
     5341 gcggcatgtt gacggtaaac agtagcgaaa actatctcgc cgccggtctt gccggtctgg
     5401 ggattattca gatcccgcgc attgccgtgc gcgaagccct gcgtgccggg cggcttattg
     5461 aagtgttgcc aggctaccgt gccgagccgc tctccctttc gctggtttac ccgcagcgtc
     5521 gggagctttc ccggcgtgta aacctgttta tgcagtggct ggctggcgta atgaaagagc
     5581 acctggactg accgactata ctttttaaga atgaccacga cagaaggaca aaagagcgga
     5641 tgacgcagga aaacgagatc aaacgtccca cccaggatct ggagcacgag ccgattaagc
     5701 agttggataa tagcgagaaa ggcggcaaag ttagccaggc gctggaaacc gtcaccacca
     5761 ctgccgaaaa agtccaacgc caaccggtta ttgcgcacct tattcgcgcg acagaacgct
     5821 ttaacgatcg gctgggtaac cagtttggtg cggctatcac ctatttctca tttttgtcga
     5881 tgatcccgat tttgatggtg tcgtttgccg cagggggctt tgttctggcc tcccatccga
     5941 tgttgctaca ggatatcttc gacaaaattc tgcaaaacat cagcgatccg acgctagccg
     6001 ccacattgaa aaacaccatc aacactgccg ttcagcagcg tacgactgta ggacttgtcg
     6061 gcctggcggt ggcgctttat tccggcatca actggatggg taacctgcgt gaagcgattc
     6121 gcgcccagtc gcgcgatgtc tgggaacgct cgccgcaaga tcaggagaag ttctgggtaa
     6181 aatatctgcg tgattttatt tcgctgattg gtttgttgat tgcgctgatt gtgacgcttt
     6241 cgatcacctc ggttgccggt tcggcgcagc aaatgattat tagcgccctg cacctgaaca
     6301 gcattgagtg gctgaaaccg acgtggcggt tgattggcct ggcgatttcc atcttcgcca
     6361 actatctgct tttcttctgg atcttctggc ggttaccgcg ccaccgtccg cgcaaaaaag
     6421 ccctgattcg cggaacattt ctggctgcta ttggttttga agtgattaaa atcgtgatga
     6481 cctacaccct gccatcgctg atgaaatccc cctctggtgc agcatttggt tccgtgctgg
     6541 ggctgatggc atttttctac ttcttcgccc gtttgacgct gttttgcgcg gcgtggattg
     6601 ccaccgccga atataaagac gacccgcgaa tgccggggaa aacgcagcct taaactaggc
     6661 cggatgcggc gtacgaattt attcggcctg gctccccgta ggccggataa gatgcgccag
     6721 catcgcatcc ggctataatg cgcacataac ctcttgaaac tcttccccag agcctcttca
     6781 gccatctatt tgggagcaaa caatttcatg ccaactcata accccagcat ataaatccag
     6841 ttggta
//
LOCUS       DAPGOF010000228         6833 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.229, whole genome shotgun sequence.
ACCESSION   DAPGOF010000228 DAPGOF010000000
VERSION     DAPGOF010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6833
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.229"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(145..630)
                     /gene="sixA"
                     /locus_tag="RHV13_004323"
     CDS             complement(145..630)
                     /gene="sixA"
                     /locus_tag="RHV13_004323"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HDV1243776.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(833..2977)
                     /gene="fadJ"
                     /locus_tag="RHV13_004324"
     CDS             complement(833..2977)
                     /gene="fadJ"
                     /locus_tag="RHV13_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HDV1243777.1"
                     /translation="MEMTSAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQKLMAEIHALPV
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLSLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKQDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPSTDRILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTEVKKDPGSDAPPTPLNSVGILGGGLMGGGIAYVTACKAGIP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(2977..4287)
                     /gene="fadI"
                     /locus_tag="RHV13_004325"
     CDS             complement(2977..4287)
                     /gene="fadI"
                     /locus_tag="RHV13_004325"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HDV1243778.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVVESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQLLAEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFAREALGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4468..4752)
                     /locus_tag="RHV13_004326"
     CDS             complement(4468..4752)
                     /locus_tag="RHV13_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HDV1243779.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKITPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5124..6464
                     /gene="fadL"
                     /locus_tag="RHV13_004327"
     CDS             5124..6464
                     /gene="fadL"
                     /locus_tag="RHV13_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HDV1243780.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTQQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGTTQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1724 a         1824 c         1608 g         1677 t
ORIGIN      
        1 aattaatagg gaagcagagg aaagagtgcg atgaaatcat catcgcagca taccaactcc
       61 gtgaagggtt tttagtgaat ttatactatt gcaaaaagac aaatgcccga tgtacagctt
      121 acatcgggca ttttttcaaa caaatcagat agctttcgcc atcttcaaat tacacgggct
      181 catctgccag ttgaatgtgc cgtttccgct ttcatcaagc gtgacgctgg caatggccga
      241 ggtggtaaac ataggcggcg tttcgcccgg acataactcg gcgaccagat acccaactaa
      301 cggtaagtgg gagataacca acaccgaagc aacaccttca ttggtcaacg cctgcaaata
      361 ggcactgacc agaccaacat cgccgcaggg cgttaactcc ggcagaactt ctgcactgga
      421 aggcaggttc agacaatccc ctacttcctc cagtgtttgc tcggctcgca ggaacgggct
      481 caccagaacg cgttcgattt ccactttttg acctttcagc cagttcgcca tcaggcgaga
      541 ttcgtcacaa ccattagtgg taagaggacg aaccgaatca ctggcggcat cgagggctgc
      601 gtcgccgtga cgcatgataa aaacttgcat attgcaccgc ttttgttaac cagattcgcc
      661 cgttttcttt catctgaaac cagagaaaga aaacggtggc cggcattgtg ccttatccat
      721 tcaccgaatg aaacgctgtt ttttacctca atggcgtaag tatagtcaat atgcgtttac
      781 attttgacca ataatccgcc attcagcgcg gattcatata gctttgacct tcttattgca
      841 ggtcagttgc agttgttttc caaaaacttt ccccacgcgc acccatctcg actaaacgct
      901 cgcaaggtgt aaaacgtgaa ccatactgtg tggccagtcg ttgcattatt gcaaccactt
      961 cgcccgcgcc gagagaatcg atatagcgaa acggtccacc gagaaatggc ggaaaaccaa
     1021 tgccaaatac cgcaccaata tccccgtcac gcacgctacg gataacctgc tcatcaacac
     1081 aacgtactgc ttcattcagc atcaacatca cacaccgttc agcaacctgc ggtgcggaga
     1141 gtcgcccctg cccttgtgcg ccaatgagcg ggtaaatggc aggatcgacc tgttttttgc
     1201 ttttacgccc tttctgacca taaagataga aaccccgacc attttttctg cctttgcgat
     1261 cgtcgttcaa aattgaagaa acaacatttg caggcgcgct aaaacgttct ccataagcgg
     1321 cttccagtac aggaataatt ttagtcccgg tgtcgattcc tacctcatcc aaaagttgga
     1381 ttggacctac cggaaaacca aatttcacta gcgcggcatc aatgtgctct acccgttcac
     1441 cttcggtcag catgcggata gcttcattaa tgtaaggcgc taagatgcga ttgacgtaaa
     1501 acccggcttt gtcacgcacg acaatgggcg ttttgccctg ttttttcgcc agttttaccg
     1561 tagtggcgat ggtttgcgct gatgtacccg catgaggaat aatctccacc agcggcattt
     1621 tttccaccgg actgaagaaa tgcaggccga taacttgctc aggtcgcgtg gcgtgagcgg
     1681 cgatatcacc aatcggtaaa gatgacgtat tcgaagcaaa gatggtatga gcggcgcaat
     1741 tttgctcaac ttccgccacc atctgttgtt tcaattcgag attttcaaac accgcttcaa
     1801 taatcagatc gcgatgggca aagccgcgat agtccgtcgt tccggagatt aatgccaact
     1861 gtttgtcacg ttcgctggct ttgagatgac gacggcgaac tttgccctcc agctgatccc
     1921 aactgtactt cagcgcatga tttatgccct gcgggttgat atctttaatt ctgaccggaa
     1981 tccccgcttt acaagcagtg acataagcaa taccgccgcc catcaagcca ccacctaaaa
     2041 tccccacgct gtttaatggc gtaggcggcg catcactgcc gggatctttc ttcacttccg
     2101 tactggcaaa aaagatacta cgcaaagctt gcgattgtgg cgtcatcgcc agttcgccaa
     2161 aggcgcgagc ttcagcgtca tagccactgc tggtgccctg cgctaatccc gtttcaacaa
     2221 cctccaggat gcggtctgtc gacggataat tgccttgagt tttgtgttct gttttcttgc
     2281 cgaccatttt gaacagcagc gcacgaccta acggccccgc cagaatacgc tcgcgtacag
     2341 gtagagggcg ggaagatgga cgatcctgct ttgccagctc aacagcagct tccagcagaa
     2401 tggagtgcgg aacgacgtca tccaccagcc ccagctttaa tgcctgtttc gcccgaagtt
     2461 gttttccggt gagaatcatc tctaatgctg tgctgacgcc tatcagacgc ggtaaacgct
     2521 gggtgccacc tgaaccgggc aacaatccaa gttgtacttc aggcaaactg agcaccgttt
     2581 taggatcgtc agtacaaacg cgaccgtggc acgcgagcgc cagctccagc ccaccgccca
     2641 ggcaagcgcc atgaatagcc gcgataaccg gaactggcaa agcatgaatc tctgccatta
     2701 acttttgccc ctgccgcgcc agagcttccg cttcttgcgc cgttttgcaa ttgccgatca
     2761 tgttgatgtc tgcgccagca atgaagttgt ccggtttagc ggagacaaac accacgcctc
     2821 gcaattcttt gttttcacgg agttgcttaa taatggcgcg cacctgcgag gcaaactccg
     2881 ccttcagggt attcattttc tcacccggta cgtcgatggt gataacggca atgttgtcca
     2941 gacgaacatt aagggtaaac gctgatgtca tttccattat tccgcctcca gaaccattgc
     3001 cgcgccaagc ccaccggcag cacaggcggt aactaaacca aatccaccgc cgcgacggcg
     3061 aagttcatgc aatgtctggg taatcatccg cgcgccggtc gccgcgaagg gatgcccgta
     3121 agcaatcgaa ccgccaagca cgttaaattt gctatcgtcc acttcgccag tggcatgtgc
     3181 acgccccagt gcttcacgag caaaacgttc actacccagc aactgaatat tcgccagtgt
     3241 ctgagctgca aaggcttcgt gcatatcgat caatgtcaga tcgctcatcg tcaaaccggc
     3301 acgctccagc gccagcggtg ttgaccaggc tggaccgagc aacatgtcct gccagacatc
     3361 gatagcggta aatgcgtagc tgcgcagata tcccagcggt accagcccta attctttcgc
     3421 ccgggattca gtcatcagga tcaccgccgc tgcgccatcg gtcagcggcg tactgttcgc
     3481 cgccgttacc gttccgtgtt tacgatcaaa cgccggacgc agctttgcgt aatcagcaag
     3541 cgaggaatta ccgcgaatat tgttgtcttc cgcgagcagt tgtttataag gagggataaa
     3601 ggcagtcatc acctcttctt tgagttttcc gtctgaccat gcctgagcgg cacgctgatg
     3661 cgaacggtgc gctaatgcat cttgctgttc tcgggtgatg ccgtaggttt tcgccatttg
     3721 ctctgcagtg tcgcccatcc gcaagccggt agaatattct gctaccgcag gtggtacggg
     3781 cattaagtcg cgcaaacgca ggcgagagaa gagtttcaga cgctggctca ttgtgcgggc
     3841 tttgttgaca tcaaccagca cgcgcgccag ttttttactg acgccaattg gcaataccga
     3901 agaggaatct gccccaccgg caatccccgc tctgatagtc cccgccatca ggctttctac
     3961 gacgtttgca accgcctgga aactggtagc gcaagcgcgg ctgacgctgt aagcatcggt
     4021 atgcacattc attccagtac cgagaacaat ttcacgcgca atgttgggag cttcaggcat
     4081 ttgtacgacc tgaccaaaga ccagttgttc aatcacttcg gcggggatct cgctgcgtgc
     4141 cagcagttcg cctaccacca tcttccctaa atcaaccgcg ggaatgccat gaaaagccgt
     4201 cgcctggcgg gcaaaaggcg tacgtaaacc gctaacaatg gcgatacgat cgccctggcg
     4261 ggtaaccagc ggtaaaacct gacccataac actcccctgt aaaaaaatta ataaagtggt
     4321 ctgacctgat catagtctta accatttttt tacatttagc caagtggaga aaagggaaag
     4381 tgggagctat gacacagaga gaaaagtaga agagaaaaga aaacgcccct gccaacaact
     4441 gacaggggcg tacgtacccg agaggaatta acgcagaccc agctggaaga tcagcatttc
     4501 agcttcgcag gcaaaagtga aatcgatatc caggcgcaca ccgtcagact cttcagtgaa
     4561 agtcggggtg attttgcacg gttcggattc cacgctacgg gctttttcag tcagcgccgc
     4621 cagcgtttgt tctgcttctg cgcggtttgc aaacacgcgg ctgtaagacg cggtgcagtc
     4681 ggagttgtcc ataatggtgc caacatccat acagcagcaa accggggttt catcagcact
     4741 acatttactc atcgttgatt tcctctgtat gtgcacccaa ggtgccagat aaacgttgtg
     4801 gatattttac gcttccggaa agtgctgctc cagttgttaa ttctgcaaaa tcggataagt
     4861 gaccgaaatc acacttaaaa atgatctaaa acaaaattca cccgaatcca tgagtgcgcc
     4921 acctccaaat tttgccagct ggatcgcgtt tcttagatca tatttgaaaa aagatagaaa
     4981 catacttgca acattccagc tggtccgacc tatactctcg ccactggtct gatttctaag
     5041 atgtacctca gaccctacac ttcgcgctcc tgttacagca cgtaacatag tttgtataaa
     5101 aataaatcat tgaggttatg gtcatgagcc agaaaaccct gtttacaaag tctgctctcg
     5161 cagtcgcagt ggcacttatc tccacccagg cctggtcggc aggctttcag ttaaacgaat
     5221 tttcttcctc tggcctgggc cgggcttatt caggggaagg tgcaattgcc gatgatgcag
     5281 gtaacgttag ccgtaacccc gcattgatta ccatgtttga ccgcccgaca ttttctgcgg
     5341 gtgcggttta tattgacccg gatgtaaata tcagcggaac gtctccatct ggtcgtagcc
     5401 tgaaagccga taacatcgcg cctacggcat gggttccgaa catgcacttt gttgctccga
     5461 ttaacgacca atttggttgg ggcgcttcta ttacctctaa ctatggcctg gctacagagt
     5521 ttaacgatac ttatgcaggc ggctctgtcg ggggtacaac cgaccttgaa accatgaacc
     5581 tgaacttaag cggtgcgtat cgcttaaata atgcatggag ctttggtctt ggtttcaacg
     5641 ccgtctacgc tcgcgcgaaa attgaacgtt tcgcaggcga tctggggcag ttggttgctg
     5701 gccaaattat gcaatctcct gctggccaaa ctcagcaagg gcaagcattg gcagctaccg
     5761 ccaacggtat tgacagtaat accaaaatcg ctcatctgaa cggtaaccag tggggctttg
     5821 gctggaacgc cggaatcctg tatgaactgg ataaaaataa ccgctatgca ctgacctacc
     5881 gttctgaagt gaaaattgac ttcaaaggta actacagcag cgatcttaat cgtgcgttta
     5941 ataactacgg tttgccaatt cctaccgcca caggtggcac aacgcaatcg ggttatctga
     6001 cgctgaacct gcctgaaatg tgggaagtgt cgggttataa ccgtgttgat ccgcagtggg
     6061 cgattcacta tagcctggct tacaccagct ggagtcagtt ccagcagctg aaagcgacct
     6121 caaccagtgg cgacacgcta ttccagaaac atgaaggctt taaagatgct taccgcatcg
     6181 cgttgggtac cacttattac tacgatgata actggacctt ccgtaccggt atcgcctttg
     6241 atgacagccc agttccggca cagaatcgtt ctatctccat tccggaccag gaccgtttct
     6301 ggctgagtgc aggtacgact tacgcattta ataaagatgc ttcagtcgac gttggtgttt
     6361 cttatatgca cggtcagagc gtgaaaatta acgaaggccc ataccagttc gagtctgaag
     6421 gtaaagcctg gctgttcggg actaacttta actacgcgtt ctgataacgc gttcgcctgg
     6481 ttaaagtcac ctgctatgca ggtgacttta actcccccac ttcaccgaaa gtagtgtcct
     6541 cattgcttgc ctcctgagtt ttgcaaacag cctgatggta gcttgccctt tcaaatcatt
     6601 aagtggtatc cacagacaaa cctgaaggga aaggcatatt ttcaggcgtt ctgctcgtct
     6661 ttctcaaaga gtttactttt ctgaatttcc aggatactca aaccaccctg gctattgtgc
     6721 gctcatacac tcaaattaaa gatagggtct aaataaatga gcgttttttg atagtctatt
     6781 tcattaggta atatatattt gtaacaaatc aatcaacatg gaataaaatc atg
//
LOCUS       DAPGOF010000229         6791 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.230, whole genome shotgun sequence.
ACCESSION   DAPGOF010000229 DAPGOF010000000
VERSION     DAPGOF010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.230"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(21..4235)
                     /locus_tag="RHV13_004328"
     CDS             complement(21..4235)
                     /locus_tag="RHV13_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDV1243781.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVQPGETDLALPGPLPFILSRTYSSYRTRTPAPVGVFGPGWKAPSDI
                     RLQLRDDGLILNDNGGRSIHFEPLLPGEAVYSRSESLWLVRGGKAAQPDGHTLARLWA
                     SLPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDVLPAPLPPYRVLTGMADRFGR
                     TLTYRREAAGDLAGEITGVTDGAGREFRLVLTTQAQRAEEARQQAVVAGAKRPDIPDS
                     LPESTEYGPDRGIRLSAVWLMHDPAYPENLPGAPLARYTYTEAGELLAVYDRSNTQVR
                     AFTYDPQHPGRMVAHRYAGRPEMRYRYDDTGRVVEQLNPAGLSYRYLYEQDRITVTDS
                     LNRREVLHTEGGAGLKRVVKKELADGSVTRSGYDPVGRLTAQTDAAGRRTEYGLNVVS
                     VDITDITTPDGRETKFYYNDGNQLTAVVYPDGLESSRAYDELDRLVTETSRSGETVRY
                     RYDDAHSELPATTTDATGSTRQMTWSRYGQLLAFTDCSGYQTRYEYDRFGQMTAVHRE
                     EGISLYRRYDNRGRLISVKDAQGHETRYEYNAAGDLTALITPDGNRSETQYDAWGKAV
                     STTQGGLTRSMEYDLAGRITTLTNENGSRSEFTYDALDRLVQQRGFDGRTQRYHYDLT
                     GKLTQSEDEGLVTLWHYDESDRLTHRTVNGEPAEQWQYDGHGWLTEISHLSEGHQVAV
                     HYGYDDKGRLAGERQTVHNPETGELLWQHETEHAYNEQGLANRVTPDGLPRVEWLTYG
                     SGYLAGMKLGGTPLVEYTRDRLHRETARSFGNGTGSTAAYELTSTYTPAGRLQSQHLN
                     SLMYDRDYVWNDNGDLVRISGPRQTREYGYSATGRLESVRTLAPDLDIRIPYATDPAG
                     NRLPDPELHPDSTLTAWPDNRIAEDAHYVYHYDEYGRLTEKTDRIPAGVIRTDDERTH
                     HYHYDSQHRLVFYTRIQHGEPQVESRYLCDPLGRRMAKRVWRRERDLTGWMSLSRKPE
                     VTWYGWDGDRLTTVQTDQTRIQTVYQPGSFTPLIRIETENGEREKMQRRSLAEKLQSE
                     GSEDGHGVVFPAELVRMLDRLEEEIRAGRVSSESRAWLAQCGLTVEQLARQVEPEYTP
                     ARKVHLYHCDHRGLPLALISEDGNTAWRGEYDEWGNQLNEENPHHVYQPYRLPGQQYD
                     EESGLYYNRNRYYDPLQGRYITQDPIGPVGGWNPYSYVLNNPAQFIDPTGLTQEDNWT
                     YGEVGNSCVTSQRDFTPPPGLADMKASIGAACSRNNASRNCHPVDGSNASAPSDKAAW
                     ENIQNATNGKDLSGGGNMFCVGNEDCAVVHSCREIVNARHPAGRVRERVTPLTTSGTA
                     TLNGTTVYFYNDPRNGNCPAHEYIPQ"
     gene            complement(4303..6411)
                     /locus_tag="RHV13_004329"
     CDS             complement(4303..6411)
                     /locus_tag="RHV13_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HDV1243782.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNPRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MEGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASDLHGEQPQAVPGR
                     RGSGTTLDNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAETIGNNQMIAVTNNQIQTVGVNQIETVGSNQIINVGSVQVETIGLVRA
                     LTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNVTFTVGKTKKDD
                     TGQTAIYSAGEHLELCCGKAKLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAA
                     SKSPPKTPDEKQDTPDMREY"
BASE COUNT         1269 a         2128 c         1800 g         1594 t
ORIGIN      
        1 cttagtctgg ccacctttct ttattgagga atatattcgt gagcaggaca attaccattc
       61 cggggatcat tataaaagta aactgttgtt ccattgagcg ttgctgttcc tgacgttgtt
      121 aacggagtta cacgttcacg cactcgtcct gccgggtgtc gagcattaac tatttcacga
      181 cagctatgaa caaccgcaca gtcctcattt cctacacaaa acatatttcc accaccagat
      241 aaatctttac catttgtcgc attttgaata ttttcccatg cggctttatc cgatggtgca
      301 gaagcattac taccatctac ggggtggcag tttctggaag cattgtttct tgagcaggca
      361 gcgccaatgc tggctttcat atccgcaagt ccgggcgggg gagtaaaatc gcgttgactc
      421 gtaacacagc tattacctac ctcaccatag gtccaattgt cctcttgagt taaacctgtt
      481 gggtcaataa actgggctgg attattaagc acataactat agggattcca cccccccact
      541 ggcccaatcg gatcctgggt aatatacctc ccctgcaacg gatcatagta ccggttgcgg
      601 ttatagtaca gccccgactc ctcatcatac tgctgccccg gcaggcggta tggctgatac
      661 acatgatgcg ggttctcctc attaagctgg ttgccccatt catcatactc cccgcgccac
      721 gccgtattgc cgtcttcgct gatgagcgcc agcggcaggc cccggtggtc gcagtgataa
      781 agatgaactt ttcgcgccgg tgtgtattcc ggctccacct gtctggccag ttgctccacc
      841 gtcagcccgc actgcgcaag ccacgcccgg ctttcactgc tcacgcggcc tgcccggatt
      901 tcttcctcca gcctgtccag catcctcacc agctcagccg ggaacaccac accgtgaccg
      961 tcctcactcc cttccgactg taacttctcc gctaggctgc ggcgctgcat tttctcccgt
     1021 tcgccgttct ctgtctcgat acggatgagc ggcgtgaagc tccccggctg gtagaccgtc
     1081 tggatacggg tctggtctgt ctgtaccgtc gtcagcctgt ctccgtccca gccataccac
     1141 gtcacctccg gtttacgcga cagcgacatc catcctgtca ggtcacgctc ccgccgccat
     1201 acccgtttcg ccattcgccg tcccagcggg tcacagaggt agcggctctc cacctgcggc
     1261 tcgccatgct gtatccgcgt gtagaacacc agacggtgct ggctgtcgta gtggtagtgg
     1321 tgggtccgct cgtcgtccgt ccgtatcaca cccgccggga tgcggtccgt cttctccgtc
     1381 agcctgccgt attcatcata gtggtaaaca tagtgcgcat cctccgcgat gcggttatcc
     1441 ggccacgccg tgagcgtgct gtccgggtgt agctccgggt ccggcagccg gtttcccgcc
     1501 gggtcggtgg catacgggat gcggatgtcc aggtccggtg cgagggtgcg cacgctctcc
     1561 agcctgcccg tggcgctgta gccgtattcc cgcgtctgtc gcgggccgct gatgcgcacc
     1621 aggtcgccgt tgtcattcca cacgtagtca cggtcataca tcaggctgtt caggtgctgg
     1681 ctctgtaacc ggcctgcggg ggtgtaagtg ctggtcagtt catatgcggc tgtactgccc
     1741 gttccgttac caaagctgcg cgcagtttcc cggtgcaggc ggtcccgcgt atactcgacc
     1801 agaggtgtcc cgcccagctt catccccgca agataaccgc tgccgtaggt cagccactcc
     1861 acccgcggca ggccgtccgg cgtgacgcgg tttgccagcc cctgttcgtt gtatgcgtgc
     1921 tctgtctcat gctgccacag cagttccccc gtctccgggt tatgcaccgt ctggcgctcc
     1981 ccggccaggc ggcccttatc atcataaccg taatgcaccg ccacctgatg gccttcgctc
     2041 aggtggctga tttctgtcag ccagccgtgg ccatcatact gccactgctc tgccggttcg
     2101 ccgttcaccg tgcggtgagt gaggcggtcc gattcgtcgt agtgccagag ggtgacaagc
     2161 ccctcatctt cactctgcgt gagttttccg gtcaggtcat agtggtaacg ttgcgtccgc
     2221 ccgtcaaagc cgcgctgctg taccagccgg tcaagcgcat cgtaggtaaa ctcactccgg
     2281 ctgccgttct cgttggtcag cgtggtgatg cgtccggcaa ggtcgtattc catactgcgc
     2341 gtcagcccgc cctgcgtggt gctgaccgcc tttccccacg catcgtactg tgtctcgctc
     2401 cggttgccgt ccggggtaat gagggcagtc aggtcgcctg cggcgttgta ttcataccgc
     2461 gtttcatggc cctgtgcgtc tttcaccgag attaaccggc cacggttgtc atagcggcgg
     2521 taaaggctga tgccttcctc gcggtggacc gccgtcatct ggccgaagcg gtcgtattca
     2581 tagcgggtct ggtagcccga gcagtcggtg aacgccagca actgaccgta gcggctccac
     2641 gtcatctgcc gggtgctgcc cgtcgcatct gttgttgtgg ccggtaattc actgtgcgca
     2701 tcatcatagc ggtagcgtac tgtctccccg ctgcgcgatg tctccgttac cagcctgtcc
     2761 agctcatcat atgcccggct gctctccagc ccgtccgggt acaccactgc cgttaactgg
     2821 ttcccgtcgt tatagtaaaa tttcgtctcc cgcccgtccg gcgtggtgat atccgtgatg
     2881 tcgactgaca ccacattcag accgtactct gtccgccgtc ccgccgcgtc cgtctgcgcc
     2941 gtaagccgcc ccaccgggtc atacccgctg cgcgtgacgc tgccgtccgc cagttctttt
     3001 ttcaccaccc gcttcagccc ggccccgcct tctgtatgca gcacctcacg ccggttcagg
     3061 ctgtcggtga cggtgatgcg gtcctgctca taaagatagc ggtagcttaa ccctgccggg
     3121 ttcagttgct ccaccacccg ccccgtatcg tcgtagcggt agcgcatctc cggccttccc
     3181 gcgtaacggt gcgccaccat ccggcccggg tgctgcgggt catacgtgaa agcacgcacc
     3241 tgcgtattgc tgcggtcata taccgccagc agttcaccgg cttccgtata cgtgtaccgc
     3301 gccagtggcg caccgggcag attctccggg tatgccgggt cgtgcatcag ccacaccgct
     3361 gaaagacgga tacccctgtc ggggccgtac tctgtgctct ccggcagtga atcaggaatg
     3421 tccggtcgtt ttgctccggc gacgaccgcc tgctgccggg cctcttccgc ccgctgcgcc
     3481 tgtgtggtca gcaccagacg gaactcacgc ccggcaccgt ccgtcacgcc ggtgatttcc
     3541 ccggccaggt caccggcggc ctcacgccgg tacgtcagcg tccgcccgaa gcggtccgcc
     3601 atcccggtaa gcacccggta cggcggcagc ggcgctggca gtacgtcctc agcacccggc
     3661 acccgctcag accaccccag tatccaccac ggcccctgtg cgctgttggt cgccaggtaa
     3721 agatgcgggc ttaaccggat atccggcggc agcgacgccc acagccgtgc cagcgtatgg
     3781 ccgtccggct gtgctgcctt accaccgcgc accagccaca gggactcgct gcggctgtac
     3841 accgcctccc ctggcagcag cggctcaaag tgaatgctcc gcccgccgtt gtcgttgagt
     3901 atcagtccgt catcacgtag ctgtaagcgg atatcagaag gcgctttcca gccggggccg
     3961 aaaacgccca ccggtgcagg cgtccgggtc cggtagctgc tgtaggtgcg ggagagaatg
     4021 aacggcagcg ggccgggcag cgcaaggtcc gtctcgccgg gctgtacttt tgcaccgagc
     4081 agcgggttca ccgggttgcc cgaggtcatc ccgcccggac acaccgagca cgccacgcca
     4141 gttggcgcgc caattcttac acctgccgaa ccctggacaa tgggaccgcc atactgagtc
     4201 atatctccct gacgcgccgc tggttttccg ctcatgattc aaattccttc ttctgtttat
     4261 ttttaatgct ggtttgttaa tgaatatcga acaggtacgg gatcagtact ctctcatatc
     4321 cggcgtatcc tgcttttcat caggggtctt cggtggggat ttcgaggctg cacagttcca
     4381 gttaatcaac agtgagtcac cattaacctg ctccttaccc tgcaaatgaa tttttgtgcc
     4441 gttgagaaaa atttgaccgt cctttgtcag caccagcttt gccttaccac agcagagctc
     4501 cagatgctca ccggcggagt aaatcgcggt ctgcccggta tcatcctttt tcgttttacc
     4561 aacggtgaag gtaacgttat ttccgacttt gacgtcataa cccagcccga ccctcaacga
     4621 tttatgcaaa ccgatctgcg aggactgcat cagagccacc gaagtgttca taatgccacc
     4681 taccgtcgtc tggtacgcca cgcccacggt cagcgcacga acaagtccaa tcgtttccac
     4741 ctgaacagaa cccacgttaa tgatctggtt actgcccacc gtctctatct ggttaacgcc
     4801 caccgtctgt atctgattgt tggtaaccgc aatcatctga ttgttgccaa tggtctcagc
     4861 atggttgttt atcacatccg tggtgcggtt attcagcacc tcggtgttca tgttcttctg
     4921 cgcgtggatg tagacctgtt ctttccctgt cgcatcgtca aacttcagtt cattaaaccc
     4981 gctgccctta taggttttcg aacgaatggt catctgcgtc ttcgtccccg gcaggctgcc
     5041 gggtgtgcgg ttttcctggt ggtaggtacg ccccataata atcggctggt ccggatcgcc
     5101 gttgaggaag tccacaatca cctcctgacc cacacgcggt atcgccaggt tgccaaagcc
     5161 ggtgcctgcc cacgcctgtg ccacacggat ccagcatgaa ctgtcctggt ttgacgggtt
     5221 atagcggtcc cagttaaatt tcacccgcac gcgaccatgt tcgtcgcaga agatttcctc
     5281 gcctgccggt cccgtcacga cggcgctctg cgggccatcc accagcggtt tcagcaacgg
     5341 ctgtggtcgc catgtccggt ctgccggtat taccgcaaaa tggttatcca gcgtggtgcc
     5401 tgaacccctg cgtcccggta ccgcctgcgg ctgttcgccg tgaagatcgc tcgccaccac
     5461 ctgccattcc cggttcaggt tcgcctgcgg atgccccgtc agcacaattc gccgtcccgg
     5521 ccatatctcc ggcgagcgac tcattccccg cgcggtttct gcattgtttc gccagccctc
     5581 catctgccag cgggcaaagt tctgcccgtg ggcgcccttg aaacgtccgg ggtagtcata
     5641 cacctcatac tgcgtgcgct ggtaatcctg gtgctggcct tcctgatcaa aacgtcctgc
     5701 ccagccgggg cgtttaaagg tgtagtcttt ggtcaccacg gaagaagggc ggatttgtgc
     5761 gctgtagcga aactggctga tgcagagggt gctcacctcg gtacgggggt tcgggttcca
     5821 ggggatttca aaagattcgg gcagatggcg gaccgtgtcg cacagcacca ggctctggtc
     5881 ggtacttttg taagcatgct cctcataaaa gaagatgcct tcctccgccg ccatccggca
     5941 caggaaatcg taatcagtct caccgtactg gacacaaaac tcacgggaag gatgcggttc
     6001 actgaacagc ggactccact cggtcacccc gttttcctgc aatatagtgc cgaggatgct
     6061 tttgatgtcc tcgttctgga agatacggaa gttctggcgc agaccggcac gccacagcgg
     6121 cgggtgcacc ttcatgctgt acagcatctg gtttttgtcg ttctccccca gttcaaacca
     6181 ggtcaccaca cctttcaccc ggcgctgtac atcatcaccc tgccatattg tcaggtaggc
     6241 cattttgtcc agcacctgcg caaattcaag ggagagaaac tgctggctga ccagggagag
     6301 atcgagggaa aagagtgaag agagtgactg ggtgagatga aaggaaacca ccgcaaaagc
     6361 gtccggtggc aggccgtcca cttccagtgt gaaacgtaat ccggttgaca tagcaacctc
     6421 cgtgaaaatg aataataatc cgcacatatt gcatgtacaa catgtcgggc ggatgagata
     6481 agcgagtcga catctggcgt taccggggaa tatcagaaaa atgtcatggt tatgtgatga
     6541 tttagtctgg gattttactt cagagaaacg ggtgtctgtt tagtcaacaa aataatcaca
     6601 aacgcaacgg atgggttctc gacgttattc acgacaggct cgtacattac agcgagtaat
     6661 aaaaaacctg atatgcaaag tcaaaccaat attcataatg tctttttatt cacaacaata
     6721 aataattcaa taacaatcat tgaaatgaat gatggtttgt ataaggaata tacgctaccg
     6781 ccccaccctg a
//
LOCUS       DAPGOF010000230         6759 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.231, whole genome shotgun sequence.
ACCESSION   DAPGOF010000230 DAPGOF010000000
VERSION     DAPGOF010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6759
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.231"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            126..914
                     /gene="fabI"
                     /locus_tag="RHV13_004330"
     CDS             126..914
                     /gene="fabI"
                     /locus_tag="RHV13_004330"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HDV1243783.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            1059..2186
                     /locus_tag="RHV13_004331"
     CDS             1059..2186
                     /locus_tag="RHV13_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HDV1243784.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEYTKSSTAELCQLAETLSLHPISLSLLERLLNSTRVNTQPDNKLAALLCARIN
                     GSPACFAACMDSSNEYKKISALVRKGENEINQWADRHSVEHATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            2254..4188
                     /gene="rnb"
                     /locus_tag="RHV13_004332"
     CDS             2254..4188
                     /gene="rnb"
                     /locus_tag="RHV13_004332"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HDV1243785.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVSTEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRFANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAMRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(4266..4370)
                     /locus_tag="RHV13_004333"
     CDS             complement(4266..4370)
                     /locus_tag="RHV13_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243786.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            4424..6409
                     /gene="pdeR"
                     /locus_tag="RHV13_004334"
     CDS             4424..6409
                     /gene="pdeR"
                     /locus_tag="RHV13_004334"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HDV1243787.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTCKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQDLIDHAINQADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSTAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            6556..6729
                     /locus_tag="RHV13_004335"
     CDS             6556..6729
                     /locus_tag="RHV13_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243788.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
BASE COUNT         1655 a         1718 c         1659 g         1727 t
ORIGIN      
        1 tggcagctat agccactcac agccaggttg attataataa ctgtttatct gttcgtactg
       61 tttatttaaa caacgaatcg cctgattttc aggcacaaca agcatcaaca ataaggatta
      121 aagctatggg ttttctttcc ggtaagcgca ttctggtaac cggtgttgcc agcaaactgt
      181 ccatcgccta cggtatcgct caggcgatgc accgcgaagg agctgaactg gcattcacct
      241 accagaacga caaactgaaa ggccgcgtag aagaatttgc cgctcaattg ggttctgaca
      301 tcgttctgca gtgcgatgtt gcagaagatg ccagcatcga caccatgttc gctgaactgg
      361 gtaaagtctg gccgaaattt gacggtttcg tacactctat tggttttgca cctggcgatc
      421 agctggatgg tgactatgtt aacgccgtta cccgtgaagg cttcaaaatt gcccacgaca
      481 tcagctccta cagcttcgtt gcaatggcaa aagcttgccg ctccatgctg aatccgggtt
      541 ctgccctgct gaccctttcc taccttggcg ctgagcgcgc tatcccgaac tacaacgtta
      601 tgggtctggc aaaagcgtct ctggaagcga acgtgcgcta tatggcgaac gcgatgggtc
      661 cggaaggtgt gcgtgttaac gccatctctg ctggtccgat ccgtactctg gcggcctccg
      721 gtatcaaaga cttccgcaaa atgctggctc attgcgaagc cgttaccccg attcgccgta
      781 ccgttactat tgaagatgtg ggtaactctg cggcattcct gtgctccgat ctctctgccg
      841 gtatctccgg tgaagtggtc cacgttgacg gcggtttcag catcgctgca atgaacgaac
      901 tcgaattgaa ataatcattc tgttggtaaa tattgggcgg cgttctgccg cccgttatct
      961 ctgttatacc tttctgatat ttgttatcgc cgatccgtct ttctcccctt cccgccttgc
     1021 gtcaggataa cgatttcctt tacgaccaag gagcgcccat ggaacaacgc cacatcaccg
     1081 gcaaaagcca ctggtatcat gaaacgcaat ccagtactgc ggagtatgac gttctgcctc
     1141 tggtcccgga agccgcaaag gtcagcgatc cctttctgct cgacgtgatc cttgatgaag
     1201 aaacgctggc tcccttcctt tcatggctgg tccctgcgcg cgttcttgca gtggaattgt
     1261 tccctgacca gcttaccgtg acccgttcac agaccttcac cgcttatgaa cgcttgtcta
     1321 cggccctgac ggttgctcag gtttgcggcg tccagcggtt atgtaactac tattcggcgc
     1381 gacttacgcc gctccccggg cctgattcct ccagggaaag caatcatagg ttggcacaaa
     1441 tcacgcaata tgcccgccaa ctggctagct cgccttctat tatcgacaac cgatcgcgcc
     1501 agcatctgaa tgacgtcggt cttactgcct gggactgtgt gatcattaac caaatcattg
     1561 gttttattgg ctttcaggcg cggaccattg cgacatttca ggcttatctc gggcacccgg
     1621 tacgctggtt acccggtctg gaaatacaaa actacgccga cgcgtcactg tttgctgatg
     1681 aatcaatacg ctggcgaagc agctatgagg tggaaaaact acctgaagag tacacaaaaa
     1741 gttcaactgc agaactttgc caactggccg aaacactctc tctccaccct atttcacttt
     1801 cccttctcga aaggttgtta aacagtacac gggttaatac acagccggat aataagcttg
     1861 cggcgttgtt atgcgcgcgg ataaatggca gtcctgcttg ttttgccgcc tgtatggatt
     1921 catcaaatga atataaaaaa atcagcgccc ttgtgcgcaa gggcgaaaat gaaattaacc
     1981 aatgggctga ccgtcattct gttgagcacg ctaccgttca ggcgatacaa tggctgaccc
     2041 gagcacccga tcgctttagc gccgcccagt tcagcccttt actcgaacac gaaaaatcat
     2101 caacgcagat tattaatctg ctggtatgga gcgggctgtg tggctggata aatcgcttaa
     2161 aaatcgcgtt gggtgagaca tattaacctt gccgcgtcag acagattcgc gtaaaactgt
     2221 cagccgctct aatggccacc aaaatagaca attatgtttc aggacaaccc gctgctagcg
     2281 cagcttaaac agcaactgca ttcccagacg ccacgcgctg aaggggtggt aaaagccaca
     2341 gaaaaaggct ttggcttcct ggaagtcgac gcgcaaaaaa gttatttcat tccgccgccg
     2401 cagatgaaaa aagtcatgca tggcgaccga attatcgcgg tgatccacag tgaaaaagaa
     2461 cgtgaatccg cagagccaga agaactggtt gaaccgttcc tgactcgttt cgtgggtaaa
     2521 gttcagggca aaaatgaccg tctggccatc gttcctgatc atccactctt aaaagacgcc
     2581 attccttgcc gcgcagcccg tggcctgaac cacgagttta aagaaggcga ctgggcggtt
     2641 gccgaaatgc gccgtcatcc gctgaaaggc gatcgttctt tctatgctga actgacacaa
     2701 tacatcactt ttggtgacga ccactttgta ccgtggtggg ttacacttgc gcgccataat
     2761 ctggaaaaag aagcaccaga cggcgtctct accgaaatgc tcgatgaagg tctggttcgt
     2821 gaagatctga ccgcgctgga ttttgtcacc atcgacagtg caagcacaga agatatggat
     2881 gacgcccttt tcgctaaggc gttgccggat gacaaacttc agctgattgt ggcgattgcc
     2941 gatccaaccg cgtggattgc tgaaggcagc aagctggaca aagccgcgaa aattcgcgca
     3001 ttcaccaact atctgcctgg cttcaacatc cctatgctgc ctcgcgagct ttctgacgat
     3061 ctctgctcac tgcgcgccaa tgaagtccgc ccggtactgg catgccgcat gacgctctcc
     3121 gctgatggca ccattgaaga taatatcgaa ttctttgccg ccaccatcga atccaaagca
     3181 aagctggtgt atgaccaggt ttctgactgg ctggaaaata ccggtgactg gcagcctgaa
     3241 agtgaagcaa ttgccgaaca agtccgtttg ctagcgcaaa tttgccaacg ccgcggcgag
     3301 tggcgtcata accacgcact ggtgtttaaa gatcgcccgg attaccgctt tattctcggt
     3361 gaaaaaggtg aagtgctgga tatcgtcgcc gagcctcgtc gctttgccaa ccgtatcgtc
     3421 gaagaagcga tgattgccgc taacatttgt gcggcccgcg tactgcgcga taagctcggt
     3481 tttggcatct ataacgtgca tatgggcttt gatccggcga atgccgacgc gctggcagcg
     3541 ttgctgaaaa cgcacggtct gcatgtcgat gccgaagaag tgctcacgct ggacggtttc
     3601 tgcaaactgc gtcgtgaact ggatgcgcaa ccaactggtt tcctcgacag ccgcattcgt
     3661 cgcttccagt catttgctga aattagcact gaacccggtc ctcactttgg cctcggtctg
     3721 gaagcatacg ccacctggac ttcgccgatc cgtaaatatg gcgacatgat caaccaccgt
     3781 ctgctgaaag cggttatcaa aggcgaaact gcgacgcgcc cacaggatga aatcactgtc
     3841 caaatggccg agcgtcgccg tctcaaccgg atggcagaac gtgatgttgg cgactggtta
     3901 tacgcacgct tcctgaaaga caaagccggg accgacaccc gtttcgcggc ggaaattgtc
     3961 gatatcagcc gtggcggcat gcgtgtgcgt ttggttgata acggcgctat cgcctttatt
     4021 ccggcacctt tcttacacgc tatgcgcgat gaactggttt gcagccagga aaacggcact
     4081 gtacaaatta aaggtgaaac ggtttacaaa gtaactgacg ttattgacgt caccattgcc
     4141 gaagtccgca tggaaacccg cagcattatt gcgcgcccgg tcgcgtaatc tcctttcacg
     4201 gcccattcct catggatggg ccgtttattt ccccgctcta ccttcgttat atccaggcag
     4261 attttttaac tgactttcgt ttgaaaactg gcgtttttcc aaaactggat tacaaataaa
     4321 tatataaaaa aaacaatgcg tttagccgcc gttaatttca tgtaagtcat gttgttcctt
     4381 ttatttaacc gcggactccg ctgttaaccg gaggatatgc atcatgaaaa ccgttaggga
     4441 gtccacaacg ttgtacaact ttctcggatc gcacaatcca tactggcggt tgacggaaag
     4501 cagcgatgtt ttgcgctttt ctaccaccga aaccacagaa cctgatcgta cattgcagtt
     4561 atctgccgaa caggctgctc gcatcaggga aatgacggtc atcacctcca gcctgatgat
     4621 gagtctgacc gtcgatgaaa gcgatctttc tgtgcatctg gtaggacgaa aaatcaataa
     4681 acgggaatgg gctggcaacg cgtctgcatg gcatgacaca ccggcggttg ctcgtgattt
     4741 atcacacggg ctttcctttg ctgagcaggt agtttctgaa gcacattccg caatagtgat
     4801 tctcgacagc cgggggaata tccaacgctt caatcggtta tgtgaagatt acacagggtt
     4861 gaaagaacac gacgtcattg ggcaaagcgt gtttaaactg tttatgagcc gtcgtgaagc
     4921 tgcggcatcc aggcgcaata accgtgtatt ttttcgaagc ggcaatgcat atgaagtcga
     4981 actgtggata ccaacatgta aaggccagcg gctgtttctg tttcgcaata aatttgtcca
     5041 cagcggcagt ggcaaaaacg agattttttt aatctgttcc ggcaccgaca ttaccgaaga
     5101 gcgccgcgct caggagcgac tgcgtattct ggcaaatacc gacagtatca ccggactgcc
     5161 gaatcgtaac gcaatgcagg atttaatcga tcacgctatt aatcaggcag ataacaataa
     5221 agttggggtt gtgtatcttg atttggataa tttcaaaaag gtcaacgacg cctatgggca
     5281 tttgtttggt gaccagttat tacgcgacgt gtcattggct attttaagct gtctcgaaca
     5341 tgaccaggtg ttggcgcgtc caggtgggga tgagtttctg gtactggcat ccaacacctc
     5401 acaaagcgcg ctggaagcaa tggcatcacg aattttgacc cgcttacggc tcccctttcg
     5461 cattggttta attgaagttt ataccagctg ttcagtaggt attgcactct ctcccgaaca
     5521 tggttcagac agcacggcta ttattcgtca cgccgacaca gcaatgtaca cagcgaagga
     5581 aggcggacga ggacaatttt gcgtttttac tccagaaatg aatcagcgag tatttgaata
     5641 tctctggctg gataccaact tgcgtaaagc actggaaaac gatcagttgg ttattcacta
     5701 tcaaccgaaa atcacctggc gtggcgaagt gcgcagtctg gaagcactag tacgttggca
     5761 gtcacctgaa cgtgggttga ttccaccgtt ggacttcatt tcctacgcag aagagtcagg
     5821 gctaattgtg cctttaggcc gttgggtgat tctcgatgtc gtacgccagg tggcaaagtg
     5881 gcgggataaa ggcattaacc tgcgagtggc ggtaaacatt tctgcacgtc agctcgccga
     5941 tcaaaccatt ttcaccgccc tgaaacaggt tctccaggaa ctcaattttg aatactgccc
     6001 tattgatgtt gaattgacag agagttgtct gattgagaat gatgaactgg cactgtctgt
     6061 tattcaacaa tttagccaac taggtgcgca agtgcatctg gatgattttg gtaccggcta
     6121 ctcttcactt tcgcaactgg cgcgctttcc gatcgatgcc atcaaacttg accaggtttt
     6181 tgttcgagat attcacaaac aacctgtctc gcagtcactg gttcgggcaa tcgtcgctgt
     6241 ggcccaggca ttgaatcttc aggtgatcgc cgaaggtgta gagagtgcaa aggaagatgc
     6301 ttttttaacc aagaacggga tcaatgagcg gcaaggattt ttgtttgcca aaccgatgcc
     6361 cgccgtcgcc ttcgaacgct ggtataaacg ctatctgaag cgcgcataag tagatgaata
     6421 tatatactta gcgctatatt ccgtcattat atatcgtatt cccggatgat aattagccgt
     6481 tgctgttaaa cacttctctt ttagcggcaa tttttgcatt attcattttc cccttttgct
     6541 ataaggatgt ccttgatgtc tgaatttgat gcccagcgcg ttgccgaacg tattgatatt
     6601 gtgttagata ttcttgtggc aggcgattac cactctgcga tccataatct cgagattctt
     6661 aaagcagaac tcttgcgcca ggttgctgaa tctacgccag acatcccgaa ggcaccgtgg
     6721 gagatttaac aatcgtgcct tcaggttttc ctatttgag
//
LOCUS       DAPGOF010000231         6665 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.232, whole genome shotgun sequence.
ACCESSION   DAPGOF010000231 DAPGOF010000000
VERSION     DAPGOF010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6665
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.232"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            160..888
                     /gene="gadW"
                     /locus_tag="RHV13_004336"
     CDS             160..888
                     /gene="gadW"
                     /locus_tag="RHV13_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HDV1243789.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(1251..4364)
                     /locus_tag="RHV13_004337"
     CDS             complement(1251..4364)
                     /locus_tag="RHV13_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HDV1243790.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGSMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(4389..5546)
                     /gene="mdtE"
                     /locus_tag="RHV13_004338"
     CDS             complement(4389..5546)
                     /gene="mdtE"
                     /locus_tag="RHV13_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HDV1243791.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            5606..5884
                     /locus_tag="RHV13_004339"
     CDS             5606..5884
                     /locus_tag="RHV13_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243792.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(5885..6412)
                     /gene="gadE"
                     /locus_tag="RHV13_004340"
     CDS             complement(5885..6412)
                     /gene="gadE"
                     /locus_tag="RHV13_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HDV1243793.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
BASE COUNT         1683 a         1622 c         1607 g         1753 t
ORIGIN      
        1 gcatttactt attatcaatt aactgttatg caaaactact ttgtggataa attttggtcc
       61 taccaaattt ggcagttttt gcgctaagaa acagtctggc atcatttcat tagtatactg
      121 aaattgaaat aatcgcagta tgaaatataa gggataatca tgactcatgt ctgctcggtg
      181 atcctcattc gtcgttcatt cgatatttat catgaacagc aaaaaatatc gctgcataac
      241 gagagtatcc tgctgctgga gaaaaatttg gcagacgatt ttgcgttttg ttcaccggat
      301 acgcgacgac tggatatcga tgagctgaca gtttgccatt acttacaaaa tattcgtcag
      361 ctaccacgca atttagggtt acatagcaaa gaccgtctgt taattaacca gtcacccccc
      421 atgccgctgg tgacggcgat ttttgatagc ttcaatgaat ccggggtaaa ttcaccgata
      481 ctgagcaata tgctctacct ttcctgttta tcgatgtttt ctcataagaa agaactgatc
      541 cccttacttt tcaatagcat cagcactgtt tcaggaaaag ttgaacgcct tattagcttt
      601 gatatcgcca aacgttggta tctgcgcgat atcgcggaaa gaatgtatac cagcgagagt
      661 ctcatcaaaa aaaagttgca ggatgaaaac acctgtttca gtaaaatatt actcgcctcc
      721 aggatgtcga tggccagacg attactcgag ttacgtcaaa ttcctctgca tactattgcg
      781 gaaaaatgtg gctatagcag tacgtcgtac tttataaaca catttcgaca atattatggt
      841 gtaacgccac atcagtttgc gcaacattcg ccaggtacct tttcctgaca tattttgcat
      901 ttgaatattg gtcaggatct cacacctgct tcatgtgaaa ctcttccctg atgatttctg
      961 ccgggctacc ggctagttct ctttcgcagt ttaccaggcg gtataggtat tttcaggagg
     1021 aaaagactcg gcatgtttgg gattattaag ctgacaattc atacgattac gggaatgtgg
     1081 gtcagtattg tgctgttcaa attgatgacg aatggctgga gtggtttcta ctttcagtgc
     1141 tgtgtactaa gcctggtatt cctgacggtt agctggttgt tgagtggtga atggttagca
     1201 ggaaagagta aggctgaacc ttcacgttca accttactct catttacacg ttacgctttt
     1261 ttaaagcggg caaagagatg ttccactaca acgaaaaaga ccggaacaaa gtaaattgcc
     1321 agcactgttg cggcaaacat cccgcccatc acgccggtac ctaccgcgtt ttgcgcgcca
     1381 gaaccggcac catgactgat aaccagcggc agcacgccga gaataaaggc cagagaggtc
     1441 atcaggattg ggcgtaaacg catccgcgcc gcttcgatga ttgcctctat cggcgttttc
     1501 ccttctttct gcatcatctc aacggcaaat tcgacaatca ggatggcgtt cttggcggaa
     1561 agcccgatgg tggtcagcaa accaacctgg aagtagacgt cattacttaa gccgcgcaga
     1621 tcggtggcca gtaatgcgcc aacgacgcct aacggaacaa ccaacatcac cgagaacgga
     1681 attgaccagc tctcataaag tgcggcgagg gcgaggaaca ccacgaccag tgaaatcgca
     1741 tacagcgcag gagcctgatt tgaggataac gcttcctgat acgacagtcc ggtccatgag
     1801 tagccgacgc ctgccggaag tttagcgacc aggtctgcca taaatttcat tgcgtcaccg
     1861 gtacttttcc cggccgccgc ttcacctaaa atctccattg acgggatgcc gttgtagcgt
     1921 tccagtcgcg gtgaaccata ggtccattcg gtagacgagt aggcagaaag cggtgccatc
     1981 gagccagagg cgttgcgtac ataccattga ttgatgttat ccggcaacat acggaacggc
     2041 gtgcctgcct ggacatacac ttttttcacc cgcccctggt tgaggaagtc gttcacgtag
     2101 ctgctgccga aggcggtgga aattgtctgg ttgatatcag acagcgccac acccattgcc
     2161 tcagctttcg cggcgttgac gttcactttg aacatcggcg tatcttccag gccgttcggg
     2221 cgtaccccgg tgacctgatt cggtgattgc gctgccagtg ataacagctc gtttcgtgcc
     2281 tgggttagtt tttcgtgccc caggttaccg ttgtccagca gttccatatc aaaacctgac
     2341 gcggtaccca gttcagccac cgcgggtaag ttgaacggga agacgacggc tttattgata
     2401 ctgcttagcg caatcattgc ccgctgaatg atcgcggtaa ccgagttttc ctcaccgaca
     2461 cgttcagacc acggcttgag actgataaac gccaggccgt tgttttgccc ctggccgctg
     2521 aagccaaagc cgccaacggt aaacaccgat tgaacattat ctttctcttt tgtcagataa
     2581 taatccgtca cctgttgcag cactttcgtg gtgttaacca tggtggcacc ggaaggtaac
     2641 tgcgcagtgg tcataaatac cccctgatcc tcttctggta agaaagaggt cggcgtgcgc
     2701 aggaacagca ccgccatccc ggcgcaaatc agcaggtaga ccaccatgta gcgaccggta
     2761 caacgcaaca gcgagcgggt gctatcggta tagtgctgag ttgatttttc aaacagcgtg
     2821 ttgaagcgtg cgaacagggc gttaggtttg tgaccgcctt ccggcgcggc tttcagaatg
     2881 gtagcgcaca gggcaggggt caggctcatt gccacaaata ctgaaagcag catggaggag
     2941 atcagcgtga tggaaaactg gcggtagatc tccccggttg caccgctcat aaaggccatc
     3001 ggcataaaca ctgcggaaag aacaacggca ataccgacca gcgcacgttg gatctgcccc
     3061 atcgatttat gcgtcgcttc cttcggcggt agcttatctt ccgcaatgac acgctcgacg
     3121 ttctccacca ccacgatggc gtcatccacc agtaacccta tcgccagcac catcccgaac
     3181 atagtcaaag tgttgatggt gaaaccgacc gccgacaaga tcgcaaacgt cccgagaata
     3241 accaccggta cggcaatcgt cgggatgatt gtggcacgga aattctgcaa aaacagatac
     3301 atgaccagga agacgaggat gatagcctca accagtgttt taaaaacttc ctgaatagaa
     3361 atttcgataa acggcgtggt gtcgtaagga taaaccgtct tcagacttgc ggggaaatag
     3421 gctgataagc ggttcagctc ctctttgact gcccgcgagg tatccagggc gtttgctccg
     3481 gtagccagtt tgatggcgat cccggcagca ggtttgccgt tatagcgcgc cacggtggaa
     3541 taatcttccg ctccaagttc gacgcgggcg acatcacgca gcagcacttg cgaaccatct
     3601 tgctgaactt tcaacaggat tttgccaaat tcttccggcg tttgcagacg cgtctgcaca
     3661 atgatcgagg cgtttagttg ctggtctgct gcctgtggca tgccacccag ttgaccacca
     3721 gaaatctggt tgttctgcac cttaatctgg gaaataacat cggaaggtac caggttatat
     3781 ttattgagtt tttgcggatc cagccagatc cgcatggcgt actcggaacc aaagagctgt
     3841 acgctaccaa cgcccgcggt acggcttagt gggtctttga tattagacgc tacatagtcc
     3901 gcgatatcgt actggttgag gctgccgtta tcagaaataa acgccgctac catcagaata
     3961 ttactgctcg acttatcaac gctaatcccc tgctgctgca ccgcttcagg taatgaaggc
     4021 atagcgagtt gcagtttatt ttgcacctga acctgtgcga tatcaggaga tgtcccagtc
     4081 tcgaaggtca gagtgataga ggcattaccc gccgcatcac tggttgaaga catgtacatc
     4141 aggccatcaa gcccattcat attttgctca atcacctgag tgaccgagtc ttctaccgtt
     4201 tgcgcatcgg cacccggata ggtagcgctg acggtaatgg tcggtggcgc aatctgcgga
     4261 tactgcgcaa ccggtaagtt catgatcgcc agaccacctg caagcatcat aataatggca
     4321 agtacccagg caaaaaccgg gcgatcaata aaatagttag ccatgaaagt ccccttaagc
     4381 ctgcaacgtt attgtttcga ttcggtgctg gcgttttcct ggctggagga aattgctcgt
     4441 gctttgatac ccggacgaat gcgttgcaaa ccggaaacga tcacccgatc gccagcctgc
     4501 aagccagagg tgacaaccca ctggtcgccg atggctttgc tggcttcaat ttcgcgtagc
     4561 tgcacgacat cgtctttatc cagaatgagc gccgttgctt taccctgggc gttgtgggtg
     4621 acgccttcct gcggcaccag taatacattc tggcggctac cttcatccac taatgccgtg
     4681 acatacatgc caggcagcaa gtcaccattt gggttgggga aaattgcccg taacgtcacg
     4741 gagcccgtgg tttcatcaac cgtcgggtcg gagaatttca gcgtgccggt ctggctgtaa
     4801 cgtttaccat tttccagatt gagctgtact ggcgtactgc cctgaacctg tttgatttgc
     4861 ccactggcga cctcttcttt catgcgtaag aaatcctgca ccgactgcgt gagatcgaca
     4921 taaatcgggt ccagacgttg tacggtaacc agcgaatctg cctgattagc ggtaacgagt
     4981 gcgccgacgg tcaccgacga tttcccgctg acgcccgtaa tcggcgaggt gacattcgcg
     5041 tattgcagat tgatcgtcgc ctgttcaaca gccgctttgg cgacggtgac attggcttct
     5101 gcttcattca actgggtgcg cgcggtgtcg taatcctgac ggctaacgta gttggtcttc
     5161 agcaacgatg cctggcggtt aaaggtgatg cgggcattgc tggcggtaga gagcgctttc
     5221 gccagcgagc ctttggcgga gtttagctcg gcctgtaaag gtgcaggatc aatctgatac
     5281 agcgaatcgc cctggttcac tttatcgcct tcgataaagt tgcgtttaat gataataccg
     5341 cccacctggg gacgtatctc ggcaacttca taaggaacgg ttctaccggg caattcgctc
     5401 aacacattga ccgacccagg ggagagtgtg acgacaccga cctcaggcgt catggcggcg
     5461 gcgttttccg ccgatttgtc atcgcaggcg gtgagcatcg cgccgcagaa taacaacggt
     5521 attaacagct ttcttcttct gttcatttta gtccctgaaa attcttgaga aataacggtt
     5581 ctttaaattg cgcgcgagga gagatatgag ggatttacag accagcggca tagtggggtt
     5641 gtcagccagt aaggtgggat acaggcatag tgatcgacat ggtgaggtca acgacaaaaa
     5701 aaatgttgcc gttctgccaa cagttcctgc cagcattcgg gcaacgaagg ggtctactgg
     5761 tggatacaca taccagggga ataaagtcgt tttagcctct cctttagtaa tcaccggggg
     5821 taatgaaatc agcatctgca tccctcgtca tgccagccat caatttcagt tgcttatgtc
     5881 ctgactaaaa ataagatgtg atacccaggg tgacgatgtc gctcatacgt cttagtttca
     5941 gcttgtacat aatgttgaat ttctgaatct ttaatgtttg ttgcgaaata ttgagcgtcg
     6001 acgtgatatt gctttcattt ttatttttca gaatcagttg cataattttg aactgattat
     6061 gtgaaaaaca taattgtgaa ttcttatggg gcaagtgttt accataagta atctcaagaa
     6121 gatttttgat actttctttg cggctaacaa aataaatagg cgctttagct tttagtttac
     6181 tgatgtgata aggtgccaaa acaataattc tctcggcatc taatttctcc agaaatttaa
     6241 tcgcttcttc atcaaggata tgattgtgat aggtatcaat gataaccttg aagggtttat
     6301 tgcctacttt tttgatctct gacagggaat tgatttttat catttcgtga ttatctttca
     6361 actgccaaaa gccctgtaaa agaaaagaat ctttcgtcat gagaaaaatc ataacttgct
     6421 ccttagccgt tatcgtttcg aacatgtcat ccttttcatt atttacatcc ttgtccgaat
     6481 cgttgttcaa tatagtaaac gcctataaga actttgccct tcgtgaacta atacgtctat
     6541 taacactaat caatacttca gatttatcca tcacgcactc catatattga tcctaagtaa
     6601 gattcctggt tgttatcagc ttgtaaatag taaacaaaaa gttaacgttt gcggattact
     6661 gccca
//
LOCUS       DAPGOF010000232         6586 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.233, whole genome shotgun sequence.
ACCESSION   DAPGOF010000232 DAPGOF010000000
VERSION     DAPGOF010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.233"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(332..2365)
                     /gene="betT"
                     /locus_tag="RHV13_004341"
     CDS             complement(332..2365)
                     /gene="betT"
                     /locus_tag="RHV13_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HDV1243794.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            2494..3081
                     /gene="betI"
                     /locus_tag="RHV13_004342"
     CDS             2494..3081
                     /gene="betI"
                     /locus_tag="RHV13_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HDV1243795.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRGKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRHQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            3095..4567
                     /gene="betB"
                     /locus_tag="RHV13_004343"
     CDS             3095..4567
                     /gene="betB"
                     /locus_tag="RHV13_004343"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HDV1243796.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILTRVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGLDNGAWVAPTVFTDCSDDMTIVREEIFGPV
                     MSILTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            4581..6269
                     /gene="betA"
                     /locus_tag="RHV13_004344"
     CDS             4581..6269
                     /gene="betA"
                     /locus_tag="RHV13_004344"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HDV1243797.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDMGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGSAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GVECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMAVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQESLPRSTAGYFVANGMPVRAKKMSRDLN"
BASE COUNT         1635 a         1812 c         1788 g         1351 t
ORIGIN      
        1 aaaacaatca ataaaatatc aaacacagac aattaaacct taaacaacaa acggttacat
       61 tgattttagt tgatttgcgc tacgcgttcc ccatgaataa agcgtcattt caaattagca
      121 ccaatattta cattaaataa gactaagctt attgaacatt cacaacagtc caaatgtagg
      181 ttttgggctt caatagttct tttctttttt attatctgtt gcctggcaat catcaatcga
      241 tactaagggc ttaaccgtac agatataacg tatcaagagg taagagaaac cgcgccatat
      301 gcaaacatgg cgcggtttta tgcaataaca atcacgcgtc cgggaacatc acgctatggc
      361 ccggcgcttc acgatggaga tgaataaagt taaggtgccg ctcgtactgg tcaagaatat
      421 cggtgatcac ctgctctttg ctgtagtcca tcaggtcgtt gccctggctg ccttctaaca
      481 ggaaggtttc cagccggtag taggtcgatt taccgcttcg tgcgcggtag gtaaagcccg
      541 gcaccgaata ttgctgcggc caaatctgat agacaaagtt ttgctcttcg cccatatgca
      601 ccaacaaatc cagatgaccc aactgctgtc cctcttccgg tggcaggctt tttagctcca
      661 cgtacgcgcc gcgcaaccgc aactcctgcg ccacttcttc cattgccggg taacagaccg
      721 tctccatcat ctgtttagtg taacgcgtgc ccggataatt catcaggcgc gagagacgtt
      781 ttttccagct caggcgatcc tgaagcccca gcggtcgcgg tgcggtatcg cggttggcac
      841 tttcacggcg gtaatcttct accttcagag atttatacaa ccccgccatc acgaagaaga
      901 tcacaaagct gaacggcagc cccataatca ccgtggtgtt ttgcagcgcg gatatcccgt
      961 tggtcatcag catgccgagc gtcagcaggc caatcgccac cgaccagaag acgcgcagcc
     1021 agccgggggc gtcgctgttg atatctttaa gctgcgaggt gaaattcccc agcaccagcg
     1081 ccccggagtc cgccgaggtc acataaaaca gcaggccagt aatggtggcg acggaggcgc
     1141 taaaggtaaa cgccggatac tgcgccagca ggctgtagaa gccgcgctcc ggatggacca
     1201 tcgcttcctc ggcaaatgcc gctccgccgt ggatgatttc atacaacgcg ctattgccga
     1261 acaccgagag ccataacagc gtgaaggtaa acggaataat caacgtgccc agcacgaact
     1321 ggcgaatggt acgcccacgc gagatacgcg ccaggaacaa gccgacaaac ggcgaccatg
     1381 ccacccacca tgcccagaag aagagcgtcc agttattcat ccactcaacc gggcggtcga
     1441 aggcaaaact gttgagcgtc atgcccataa agcgattcac atagtcgcca acattcagca
     1501 ccagtgcatt aagcaggaac gaagtgtcgc ccataaacaa cacgaacagg atcaatccca
     1561 gcgccagcgc gacattaagc tccgataaca cgcgaatgcc cttatcgaca ccggatgtca
     1621 ccgagatagt ggcgattatc accgacaagg cgatcagtgc cgcttttgcc gccatcgaat
     1681 cgggaatatc aaacagtacg ctcaagccat agttaagctg caccacaccg ataccgagcg
     1741 tagtggcaat accgaagata gtgccgatca ccgccgcgat atccactgag tgacctatcg
     1801 gcccgttaat ccgtttaccg aagatcgggt acagtgccga gcggatggtg agcggcaaat
     1861 tataacgata gctaaagtat ccgagcgcca tgcccatcag cgcatacatc gaccagccgg
     1921 ttaagccgta gtgaaacagc gtccagacca tcgcctgacg cgcggcctca atcgtctgtc
     1981 ccgcgccttc tggcggctgc atatactgcg ttaccggttc ggctacggaa aagaacatca
     2041 gatcgatacc gatcccggca gcaaacagca tcgccgccca actcagcagg ctgaattccg
     2101 gtttggattg ttctggcccg agcttcaccg aaccaaaacg cgaacaagcg atacagacca
     2161 caaagacaat atagagcgtt gccgccagca gatagtacca accgaaggtt ttagaaaccc
     2221 agtccagcgt gcggccaatc cacagggccg agaagtcgcg aaacaggatc gttgtcaggg
     2281 aaaacaacaa aatcagtccg gcggaagtgt aaaacaccac cggattgatt ttgtcctttt
     2341 ccctgctgtg tgaaaggtct gtcatccagt atccccactg ttattgttac catttaaaat
     2401 caaattcgta acaattaaga cacattttat attgaacgtc caatcaataa ccgctttaat
     2461 agataaacaa cgctgatgaa tggagtggcg aaaatgccca aattggggat gcagtcgatc
     2521 cggcgcagac aactgatcga cgccacactg gaagcaataa atgaagtggg catgcacgat
     2581 gcaacgatcg cgcagatcgc ccgccgtgca ggcgtttcta cggggatcat cagccactat
     2641 ttcaggggca aaaatggtct gctggaagca accatgcgcg atatcaccag tcagctgcgt
     2701 gacgcggttt tgaatcgatt acatgcactt ccgcagggca gtgcagagca gcgattacag
     2761 gcgattgttg gcggaaactt cgatgaaacg caggtgagca gtgcggcgat gaaagcctgg
     2821 ctggcgttct gggccagcag tatgcatcag ccgatgctct atcgtttaca gcaggtcagc
     2881 agccgccgct tgctgtcgaa tctggtgagc gagtttcgcc gcgaattgcc gcgccaccag
     2941 gcacaggaag cgggctacgg ccttgccgcg ctgattgacg gattatggct gcgcgcggca
     3001 ctgagcggca aaccgctgga taaacctctc gcacactcgc tgacccgtca ctttattact
     3061 cagcatttac ccaccgatta accgaggaga cgtgatgtcc cgaatggcag aacagcagct
     3121 ttatatacat ggtggttata cctccgccac cagcggtcgc accttcgaga ccattaaccc
     3181 ggccaacggt aacgtgctgg cgaccgtgca ggccgccggg cgtgaggatg tcgatcgcgc
     3241 cgtgaagagt gcccaacagg ggcaaaaaat ctgggcggcg atgaccgcta tggaacgctc
     3301 gcgtattctg cgtcgggccg ttgatattct gcgtgaacgc aatgacgaac tcgcaaaact
     3361 ggaaaccctc gacaccggaa aagcatattc ggaaacctca accgtcgata tcgttaccgg
     3421 tgcggacgtg ctggagtact acgccgggct gatcccggcg ctggaaggca gccagatccc
     3481 gttgcgtgag acgtcatttg tttatacccg ccgcgaaccg ctgggcgtgg tggcggggat
     3541 tggcgcatgg aactacccga tccagattgc cctgtggaaa tccgccccgg cgctggcggc
     3601 aggcaacgca atgattttca aaccgagcga agtcaccccg cttaccgcgt taaagctggc
     3661 tgaaatttac agcgaagcgg gcctgccgga cggcgtattt aacgtgttgc cgggcgtggg
     3721 cgcggagacc gggcaatatc tgaccgagca tccgggcatt gccaaagtgt catttactgg
     3781 cggtgtcgcc agcggcaaaa aagtgatggc taactcggcg gcctcttccc tgaaagaagt
     3841 gaccatggaa ctgggcggta aatcaccgct gatcgttttc gatgatgcgg atctcgatct
     3901 cgccgccgat atcgccatga tggcaaactt cttcagctcc ggtcaggtgt gtaccaatgg
     3961 cacccgcgtc ttcgttccgg cgaaatgcaa agccgcattt gagcaaaaga ttctcacgcg
     4021 cgttgaacgc attcgcgcgg gcgacgtttt cgatccgcaa actaacttcg gcccgctggt
     4081 cagcttcccg catcgcgata acgtgctgcg ctatatcgcc aaaggcaaag aggaaggcgc
     4141 gcgcgtactg tgcggcggcg atgtactgaa aggcgatggc ctcgataacg gcgcatgggt
     4201 cgcgccgact gtgttcaccg attgcagcga cgatatgacc atcgtgcgtg aagagatctt
     4261 cgggccagtg atgtccattc tgacctacga gtcggaagac gaagtcattc gccgcgctaa
     4321 cgataccgac tacggcctgg cggcgggaat cgtgacggcg gacctgaacc gcgcgcatcg
     4381 cgtcattcat cagctggaag cgggtatttg ctggatcaac acctggggcg aatccccggc
     4441 agagatgccc gttggcggct acaaacactc cggtattggc cgcgagaacg gcgtgatgac
     4501 gctccagagt tacacccagg tgaagtccat ccaggttgag atggctaaat tccagtccat
     4561 attctaacca ggaggtttat ttgcaatttg actacatcat tattggtgcc ggctcggctg
     4621 gcaacgttct cgctacccgt ctgactgaag atccgaatac ctccgtgctg ctgcttgaag
     4681 cgggcggccc ggactatcgc tttgacttcc gcacccagat gcccgctgcc ctggcattcc
     4741 cgctacaggg taaacgctac aactgggcct atgaaacgga acctgaaccg tttatgaata
     4801 accgccgcat ggagtgcgga cgcggtaaag gtctgggtgg atcgtcgctg atcaacggca
     4861 tgtgctacat ccgtggcaat gcgctggatc tcgataactg ggcgcaagaa cccggtctgg
     4921 agaactggag ctacctcgac tgcctgccct actaccgcaa ggccgagact cgcgatatgg
     4981 gcgagaacga ctaccacggc ggcgacggcc cggtgagcgt caccacctcc aaacccggcg
     5041 tcaatccgct gtttgaagcg atgattgaag cgggcgtgca ggcgggctac ccgcgcacgg
     5101 acgatctcaa cggttatcag caggaaggtt tcggcccgat ggatcgcacc gttacgccgc
     5161 agggccgccg cgccagcacc gcgcgcggtt atctcgatca ggccaaatcg cgcccgaacc
     5221 tgaccattcg tactcacgct atgaccgatc acatcatttt tgacggcaaa cgcgcggtag
     5281 gcgtcgagtg gctggaaggc gatagcacca ttccaacccg cgcgacagcg aataaagagg
     5341 tgctgttatg tgcaggtgcg atcgcctctc cgcagatcct gcaacgttcc ggcgtgggta
     5401 gcgctgaact gctggcggag tttgatattc cgctggtgca tgaattaccc ggcgtcggcg
     5461 aaaatcttca ggatcacctg gagatgtatc tgcaatatga gtgcaaagaa ccggtttccc
     5521 tctaccctgc cctgcagtgg tggaatcagc cgaaaatcgg tgcggagtgg ctgtttggcg
     5581 gcaccggcgt tggtgccagc aaccactttg aagcaggcgg atttattcgc agccgagagg
     5641 aatttgcgtg gccgaatatt cagtaccatt tcctgccagt agcgattaac tataacggct
     5701 cgaatgcagt gaaagagcac ggcttccagt gccacgtcgg atcgatgcgc tcgccaagcc
     5761 gtgggcatgt gcggattaaa tcccgcgacc cgcaccagca tccggcgatt ctgtttaact
     5821 acatgtcgca cgagcaggac tggcaggagt tccgcgacgc aattcgcatc acccgcgaga
     5881 tcatgcatca accggcgctg gatcagtatc gtggccgcga aatcagcccc ggtgtcgaat
     5941 gccagacgga tgagcaactc gatgagttcg tgcgtaacca cgccgaaacc gccttccacc
     6001 cgtgcggtac ctgcaaaatg ggttatgacg agatggcagt ggttgacggc gaaggccgcg
     6061 tgcatgggct ggaaggcctg cgcgtggtgg atgcgtcaat tatgccgcag attatcaccg
     6121 ggaatttgaa cgccacgaca attatgattg gcgagaaaat agcggacatg attcgcgggc
     6181 aagaatcgct gccgaggagc acggcgggat attttgtggc gaatgggatg ccggtgagag
     6241 cgaaaaaaat gagtcgtgat ttgaactgac tcaggaaccg atcagattga gagttaccgt
     6301 tccaggtatg gggacagaat cgttatataa aacatagggg taactctcat gcttcatagt
     6361 gacaacctcg ccagcaaatt ctaagctgac tcgttcaatc tcgctgatga aggtactatg
     6421 aatgcactga taaaccgcag gtgaaatgcc actcagctta agaattacgc cgctagtgtt
     6481 cctggctatg gtgacttgtt cactcccgct cacggttagc atatacgaaa agggttcttg
     6541 taacgaggac aaaaataagt cagaccaaaa caacgtggag ctttaa
//
LOCUS       DAPGOF010000233         6511 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.235, whole genome shotgun sequence.
ACCESSION   DAPGOF010000233 DAPGOF010000000
VERSION     DAPGOF010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.235"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            19..582
                     /gene="fimA"
                     /locus_tag="RHV13_004345"
     CDS             19..582
                     /gene="fimA"
                     /locus_tag="RHV13_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HDV1243798.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
     gene            943..1653
                     /locus_tag="RHV13_004346"
     CDS             943..1653
                     /locus_tag="RHV13_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDV1243799.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDITHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            1695..4346
                     /locus_tag="RHV13_004347"
     CDS             1695..4346
                     /locus_tag="RHV13_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDV1243800.1"
                     /translation="MTAFRAAFKAYRMHQVLIMPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFIADDNNAELIP
                     CLSTDLLVSLGIKKSALLDNKEHSAEKHVPDNSACTPLQDRLADASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPRFIQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADGTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDTNEQTQFIDYFNLFYSKRGQEQISISQQLGNYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRHSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGNDK
                     NSNCIVDYKLPEVSPGTLLNQQTAICR"
     gene            4360..4890
                     /locus_tag="RHV13_004348"
     CDS             4360..4890
                     /locus_tag="RHV13_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV1243801.1"
                     /translation="MKYNNVIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            4903..5406
                     /locus_tag="RHV13_004349"
     CDS             4903..5406
                     /locus_tag="RHV13_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV1243802.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASSWHNITLSLTDCPAETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDAALKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            5466..6380
                     /locus_tag="RHV13_004350"
     CDS             5466..6380
                     /locus_tag="RHV13_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV1243803.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNMVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVNLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSARQVFANTAPDATKASGVGVSLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
BASE COUNT         1856 a         1395 c         1458 g         1802 t
ORIGIN      
        1 tataaatttt ggagaattat gaaactcaaa catgttggta tgattgtcgt ttctgtgttg
       61 gcgatgtcgt ctgctgcggt aagcgcagcc gagggtgatg aatcagtaac gaccactgtt
      121 aatggcggtg ttattcattt taaaggtgaa gtggtaaatg ccgcttgtgc aattgattca
      181 gaatcaatga accaaacggt tgagctgggt caggttcgtt cttctcgcct ggctaaagcg
      241 ggtgacctca gctccgccgt tggcttcaat atcaagctaa atgattgtga taccaatgtt
      301 tccagtaatg cagctgttgc attcctgggt actactgtca ccagtaatga cgatacgtta
      361 gcgctgcaga gttcagcggc aggctctgcc caaaatgtcg gtattcaaat tttggaccgt
      421 acgggtgagg tattaatact tgatggggcc acttttagtg ctaaaaccga cttgattgat
      481 ggcacgaata tactgccatt ccaggctcgt tatattgctc tcgggcagtc cgtagctggt
      541 actgcaaacg ctgatgcgac cttcaaagtt caatatctat aattcaatct taaggtggct
      601 atacgtcatc tgaaattgaa acagggtgtg tgtggcatgg ctacgcacac ccgagcaatt
      661 taccaatatt catctcattt ttaatgagaa atggtttgta tttggagcgc tatgaaaaat
      721 cacataatat ctaaactatc aataaacaac agtagttaaa cttactgtta tttaaaatat
      781 ttactttgta gcaaggtttt cctattaaaa gagaaccaac atttattaca aacgcattaa
      841 gtctggaaac ccggcgactc gccaatgtat tagcaacatt aatcgattgt ctgatgtaac
      901 ggttttcccg cttatcctct taacacatgg agaatgcatc agatgcaaac tacgcgtacc
      961 ccctactcta tttcctttat ggcgacagtg ttacttttat tgctctttgc ttgccatagc
     1021 accgttgcca acgcagctgt cgcactgggt gcgacgcggg taatttatcc ggcaaaccag
     1081 aaacaggttt tattgcctgt cacgaataat gatcctgcca gtgtctatct cattcagtca
     1141 tggatagaaa atgcaggtga ccaaaaagac acccagtttg tcattacgcc accgcttttt
     1201 tccatgcaag gtaaaaaaga gaatacgctg cggataatca acgctactaa tcatcagtta
     1261 ccaggcgacc gtgaaagcct gttctgggtg aatgttaaag ctattcctgc tatggaaaaa
     1321 gaccagaaga atgagaatac attacagctg gcaattatta gccgcataaa aatgttttat
     1381 cgccccacga acctggcaat ggcacctgaa gaagcgcctg caatgcttcg gtttcgtcgc
     1441 agcggtagca aactcacgct tattaatcca acgccctatt tcattaccgt aacaaatatg
     1501 aaagcaggaa atagcaatct cccaaacacc atggtgccgc caaaaggtga agtatcggtg
     1561 gatatcacgc atgctgcaac cggggatatc agttttcaga cgatcaatga ttacggtgca
     1621 ttaactccgc gtattaaggc aaccatgcag taagccagtg acctaattaa taataaactc
     1681 aatcggaatt aataatgaca gcctttcgtg ccgcatttaa ggcatatcgt atgcatcagg
     1741 tgttaataat gcctcgcttt gcacggctta ccattgccct tggcttagca actgcggtat
     1801 ttcccgttga cgcagagtat tattttaatc cacggttttt aagcaatgat cttgctgaat
     1861 ctgtcgattt atccgccttt actaagggac gcgaggcacc acctggtaca tatcgggtcg
     1921 acatttattt gaacgatgaa tttatgacta gtcgggatat tacttttatt gcagatgata
     1981 ataatgcaga actcatccca tgcctgagta cagatcttct tgtcagtctt ggtataaaaa
     2041 aatcagcgtt attagacaat aaagaacatt cggcagaaaa acatgtgccg gacaacagcg
     2101 catgcacacc acttcaggat cgtttggccg acgcatcaac tgagtttgat gttggtcagc
     2161 aacatttgtc actctccgtt ccgcaaattt atgttggcag aatggctcgc ggctatgttt
     2221 ctcctgatct gtgggaagaa ggaataaatg ctgggcttct aaactatagt tttaatggta
     2281 attctattaa taatcgtagt aaccataatg caggaaaatc caactatgca tatttgaatt
     2341 tacagagtgg catcaacatt ggtagttggc gactgcgcga taactcaacg tggagttata
     2401 acagtgggag tagcaattca tctgacagca ataaatggca gcatatcaat acgtcggctg
     2461 aacgtgacat tattccctta cgctcacggt taacggtagg tgatagttat accgatggcg
     2521 atatttttga tagtgtgaac tttcgtggcc tcaaaataaa ttcaacagaa gcgatgttgc
     2581 ccgatagcca acatggtttc gctccggtga ttcatggtat tgcccgtggc accgcacaag
     2641 tgagtgtaaa acagaacgga tacgatgttt atcagactac tgttccaccc ggccctttta
     2701 ctattgatga tatcaactct gcggccaatg gtggtgatct gcaagtaacc ataaaagagg
     2761 cagacggcag tattcagaca ttatatgttc cttattcgtc tgttccggtt ctccaacgtg
     2821 ctggatatac gcgttatgcg cttgccatgg gggaatatcg tagtggaaat aacctgcaaa
     2881 gctcccccag gttcatacaa ggtagcttga tgcatggtct ggaaggaaac tggacacctt
     2941 atggcggaat gcaaattgca gaagattatc aggccttcaa ccttggtatt gggaaagatt
     3001 taggactttt tggtgccttt tctttcgata tcacgcaggc caatacgaca cttgcagatg
     3061 gcacccgtca cagtgggcaa tcggttaaat ccgtctacag caaatccttc taccagacag
     3121 gaaccaatat ccaggtcgca ggatatcgct attctacgca aggtttttat aacttatccg
     3181 acagtgccta cagtcgaatg agtggttaca ccgtcaagcc tcctaccgga gacaccaatg
     3241 agcagacaca atttattgat tattttaatc tgttctacag taagcgtggt caggaacaaa
     3301 taagcatctc tcagcagctt ggaaattacg gtacgacatt tttcagtgcc agtcgccaaa
     3361 gttactggaa cacgtcacgc agcgaccagc aaatatcatt tggattaaat gtgccgtttg
     3421 gtgatattac gacttcgctg aattacagct attccaataa tatatggcaa aacgatcggg
     3481 atcatttact cgcttttacg cttaatgttc ccttcagtca ttggatgcgt acagacagtc
     3541 agtcggcatt tcgtcattca aacgccagtt acagtatgtc aaacgatttg aaaggcggca
     3601 tgaccaatct atcgggggtt tatggcactc tgctgccgga taataacctg aattatagcg
     3661 ttcaggtcgg taacacccac ggaggtaata catcgtctgg caccagtggt tacagttctc
     3721 ttaattatcg tggagcttac ggtaatacga atgtcggtta cagtcggagt ggtgacagca
     3781 gccagattta ttacggaatg agtggtggga ttattgctca tgctgatggc atcacctttg
     3841 gacagccgct gggcgacaca atggttctgg ttaaggctcc tggcgctgat aatgtcaaaa
     3901 tagagaacca gaccggaatt cataccgact ggcgtggcta tgccatatta ccatttgcga
     3961 cagaatatag agaaaatcgt gtcgctctta acgcgaattc ccttgcagat aatgttgaac
     4021 tggatgaaac cgtggtcact gtcatcccaa ctcacggtgc tattgccaga gcaacattta
     4081 atgcacaaat cggcgggaaa gtattaatga cgttgaagta cggtaataaa agcgttccat
     4141 tcggtgcaat tgtcactcac ggagagaata aaaatggcag cattgtcgcg gaaaatggtc
     4201 aggtttatct gactggactt ccacagtcag ggaaattaca ggtttcatgg ggcaatgata
     4261 aaaactcaaa ctgtattgtc gattacaagc ttcctgaagt ctctcctgga accttgctga
     4321 accagcagac agcaatctgt cgctaaggaa caatagatta tgaaatacaa taacgttatt
     4381 ttcctcggtt tatgtctggg gttaaccacc tattctgctt tatccgcaga tagcgttatt
     4441 aaaattagcg ggcgcgtcct cgattatggc tgcacagtct catcggattc gcttaatttt
     4501 accgtagatc tccaaaaaaa cagtgccaga caatttccaa cgaccggtag cacaagtcca
     4561 gccgtccctt ttcagattac gttaagtgaa tgcagcaaag ggacaacggg ggttcgggtt
     4621 gcatttaacg gtattgagga tgcagaaaat aatactctgt tgaaactgga tgagggaagc
     4681 aatacggcct ccggtttagg tatagaaata ctggacggaa atatgcgtcc ggtgaaactg
     4741 aatgaccttc atgccgggat gcagtggatc ccactggtac cagaacagaa caatattttg
     4801 ccttactccg ctcgtctgaa gtcaactcag aagtccgtca atccgggact ggtgagggct
     4861 tcggcaacct ttacccttga atttcaataa tcagagtcag atatgaaaag acttcacaag
     4921 aggttcctgt tagctacgtt ttgcgcgtta ttcacagcaa ctctccaggc cgccgatgtc
     4981 actatcactg ttaatggtcg ggtagtcgct aaaccctgca ctattcaaac caaagaagct
     5041 aacgttaatc tcggggatct ttatacgcgc aatctgcaac aacctggttc tgcatctagc
     5101 tggcacaata ttactttgtc attaaccgat tgtccggctg aaacaagtgc agtgacggca
     5161 atcgtgacag gttcaactga caatacgggt tattacaaaa atgaaggtac tgccgaaaat
     5221 attcagatag agctgaggga tgaccaggat gctgcgttaa aaaatggcga tagcaaaacg
     5281 gttattgttg atgagatcac tcgtaatgca cagtttccac ttaaggcaag agctatcacg
     5341 gtgaatggaa acgcaagcca gggaacgatc gaggcgctaa tcaatgtgat ctacacctgg
     5401 caataacgtc gattcgattt tcttagcctt ctttttaaat caatttttta accttaggat
     5461 atatcatggg taaaacaatc agtataaagg tcctgttcgg tatttatctt ctattaatgg
     5521 caggcaaagt ttttgccttc agttgtaatg tcgatggcgg gtcaagtatt ggagcaggta
     5581 caacctctgt ttatgttaat ctcgaccctg taatacaacc gggacagaat atggttgtag
     5641 acttgtctca gcatataagt tgctggaatg attacggcgg ctggtacgat actgatcata
     5701 taaacctggt acaaggctca gctttcgcag gctccctaca gtcatataaa ggatcgctgt
     5761 actggaataa tgtgacctac cccttcccgt tgacgacaaa taccaacgta ttggatattg
     5821 gtgataagac accgatgcca ttaccgttaa aactctatat taccccagtc ggggcggccg
     5881 gtggagttgt cataaaagcc ggggaagtta ttgcccgtat tcatatgtat aaaattgcca
     5941 cgttaggaag cggaaatccg cgtaatttta cgtggaatat tatttcaaat aacagtgttg
     6001 ttatgccaac aggcggctgc actgttgata gtcgtaatgt caccgtgaat ctcccggact
     6061 ttcctggaag cgcagaaatc ccccttggcg tatattgctc aagtgaacaa aagctctcat
     6121 tttatctttc gggtgccacc actgacagtg cccgacaagt gtttgctaac acagcaccag
     6181 atgcaacaaa agcatcggga gtgggtgtct ctctgatgcg taatggaaaa atactggcta
     6241 cgggagaaaa tgtctccttg gggacagtaa ataaaagcaa agtacctctg ggtttaagcg
     6301 caacctatgg acaaacaggg aacaaagttt cagccggaac cgtccagtca gtcattggtg
     6361 tcacttttat ctatgaataa ttgtgatttt gaccttagtc tatgcgctcc attcacgaaa
     6421 attgcgcgga taattatttt gtgaaggcta ttatcctaca cctgtaagaa aatccgcgca
     6481 caattgaagc cagagtctta ttttttatat t
//
LOCUS       DAPGOF010000234         6378 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.236, whole genome shotgun sequence.
ACCESSION   DAPGOF010000234 DAPGOF010000000
VERSION     DAPGOF010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.236"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            44..1960
                     /gene="mngA"
                     /locus_tag="RHV13_004351"
     CDS             44..1960
                     /gene="mngA"
                     /locus_tag="RHV13_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HDV1243804.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRIVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGMLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            1978..4611
                     /gene="mngB"
                     /locus_tag="RHV13_004352"
     CDS             1978..4611
                     /gene="mngB"
                     /locus_tag="RHV13_004352"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HDV1243805.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEQIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEIIHEAWQSRAVIR
                     YEMAVPRNLQERSARQSTGRVGVELVVTLSHNSRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGSFSC
                     RLSLLSYTGTPATAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVVLRLFNPAESTTCEATVAFSREVISCTQTMMDESISTAERD
                     NLKELGAFLPGQSRTFSYRLG"
     gene            complement(4756..6153)
                     /locus_tag="RHV13_004353"
     CDS             complement(4756..6153)
                     /locus_tag="RHV13_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HDV1243806.1"
                     /translation="MHHLSRLDKAFLRKGIQLSRANNIVSLSRPSGASARVLLPDELP
                     LEEKAVTQLLDFAEVKLPDHPGYVRQTCATPDFHPGNGVPVGAVVASTPDLVIPAAIG
                     TDISCGMRLLTTGLTLEQAEANKDVITQRLTQVLLQNERDVPLSAQAFTALFDGGPAD
                     YVSTLPSAGLWTRTNIPRMVQEIDRCIGLNEHNSHSRYAPPALLATDRALLRDPCLGT
                     PGSGNHFIELQVVDSVVDRHVAWQQGLKTGDIVVMIHSGSRDVGFYVGRRWMDRARAE
                     WPAGHKHPQSGLYGLAGGLAEEYLQAMGMAARYAWLNRVTIAEMVRVCFADLFHQDDS
                     HLVVDVPHNIILREHEMNIHRKGATPAHAGALALIPGSMGDYTWLAVGCGNSEWLWSC
                     SHGAGRSQRRQAMRSRATEESTLPWQCVTLREERRLEEAPAAYKDIAPVIEAQQEAGL
                     IQPAVRLRPWLTFKG"
BASE COUNT         1549 a         1537 c         1746 g         1546 t
ORIGIN      
        1 ggcacactgg cgcgactata aaaacgatca agtgaggatc atgatgaatc tgacgactct
       61 gacccaccgc gatgcgttgt gtctgaatgc gcgctttacc agccgtgaag aggccatcca
      121 cgcgttgact caacgtcttg ctgctctggg gaaaatttcc agtactgagc aatttctgga
      181 agaagtgtat cgccgtgaaa gccttggccc gaccgcctta ggtgaagggt tggctgtgcc
      241 gcatggcaaa actgctgcgg taaaagaagc ggcgtttgcg gtcgccacac tcagcgagcc
      301 gcttcagtgg gaaggcgttg atggcccgga agcggttgat ttagtggtgt tgctggcgat
      361 tccccccaat gaagcgggta ctacgcatat gcaactgctg acagcgctga ccacgcgcct
      421 ggcggatgat gagattcggg cgcgtataca gtcggcgacg acgcctgatg aattgctctc
      481 ggcgctggat gacaagggag gcacgcaacc ttctgcctct ttttccaacg cgccaactat
      541 tgtctgcgta acggcctgcc cggcgggtat tgctcacacc tacatggctg cggagtatct
      601 ggaaaaagcc ggacgcaaac tcggcgtgaa tgtctacgtt gaaaagcaag gtgctaacgg
      661 cattgaaggg cgtttaacgg cggatcaact caatagtgca actgcctgta tttttgcggc
      721 tgaagtcgcc atcaaggaga gtgagcgttt taacggcatt cccgcgcttt cggtgcctgt
      781 tgccgagccg attcgccatg cagaagcttt gattcaacaa gcgcttaccc tcaagcgtag
      841 cgatgagacg cgtatcgtac agcaagatac gcaaccggtg aaaagtgtca aaacggagct
      901 gaaacaggca ctgttgagcg ggatctcttt tgccgtaccg ttgattgtcg cggggggcac
      961 ggtgctggcg gtcgcggtat tactgtcgca aatcttcggg ctacaagatc tgtttaatga
     1021 agaaaactcc tggctgtgga tgtaccgcaa gctgggcggc gggatgctcg ggattttgat
     1081 ggtgccggtg ctcgcggcct ataccgccta ttcactggca gataaaccgg cgttagcgcc
     1141 aggctttgcg gctggacttg ccgccaacat gatcggttcc gggtttctcg gcgcggtcgt
     1201 tggcggattg atagccggtt atctgatgcg ctgggtgaaa aatcacttgc gtcttagcag
     1261 taaattcaat ggattcctga ctttttatct ctacccggtg ctcggtacat tgggagcggg
     1321 cagtctgatg ctgtttgtgg tgggggaacc tgtcgcctgg atcaataact cgcttaccgc
     1381 ctggctgaac ggtctgtcag gaagtaacgc gctgttgctg ggtgccattc tcggttttat
     1441 gtgttccttt gaccttggag ggccagtgaa caaagccgct tatgcattct gcctgggcgc
     1501 aatggcgaac ggcgtttatg gcccgtatgc cattttcgcc tccgtcaaaa tggtttcggc
     1561 atttaccgta accgcttcca cgatgctcgc accgcgcctg tttaaagagt ttgaaattga
     1621 gaccggaaaa tccacctggc tgttagggct ggcaggtatt accgaagggg cgatcccgat
     1681 ggcgattgaa gatccgctgc gggttattgg ttcgtttgtg ctgggctcta tggtaacggg
     1741 cgctattgtc ggcgcgatga atatcggcct ttcgacaccc ggtgccggca ttttctcgct
     1801 ctttttactt catgataatg gcgcgggcgg tgttatggcg gcaattggct ggtttggtgc
     1861 ggcattggtg ggggctgcaa tctcgactgc aattctcctt atctggcggc gtcacgcggt
     1921 taagcatggc aactatctga ctgatggcgt aatgccataa acaaaaagga aacgacgatg
     1981 aaagcggtat ctcgcgttca catcaccccg catatgcact gggatcggga gtggtatttc
     2041 accaccgaag agtcacgcat tctgctggtc aataatatgg aagagatcct gtgccgactg
     2101 gaacaggaca acgaatacaa atattacgta ctcgacgggc aaacggcgat cctcgaagat
     2161 tatttcgcgg tgaaaccgga aaacaaagac cgcgtgaaga aactggtaga agccggcaag
     2221 ttgattatcg gcccctggta tacccagacc gataccacga ttgtttctgc ggaatccatc
     2281 gtccgtaatc tgatgtacgg aatgcgtgac tgcctcgcgt ttggcgagcc gatgaaaata
     2341 ggttatttac cggattcctt tggcatgtcc gggcaactgc cgcatatcta caatggattt
     2401 ggcattaccc gcaccatgtt ctggcgtgga tgttctgagc gccacggtac tgataaaacc
     2461 gagtttttgt ggcaaagcag tgacggtagc gaagtgacgg cgcaggtgct gccgttgggt
     2521 tatgccatcg gtaagtactt acctgcggac gaaaacggat tacgcaaacg cctcgacagt
     2581 tattttgacg tgctggaaaa agcgtctgta accaaagaga ttttgctgcc gaatggtcat
     2641 gaccagatgc cattgcagca aaatatcttc gaagtaatgg agaaacttcg cgaaatttat
     2701 cctcaacgaa agtttgtgat gagccgcttt gaagaggtat tcgagcagat tgaagcgcag
     2761 cgtgaaagtc tggcgaccct gaaaggtgaa tttattgatg gcaaatatat gcgcgtgcat
     2821 cgcaccatcg gttccacgcg catggatatc aaagttgccc acgcacgtat tgaaaataag
     2881 attgttaatc tgctggaacc gctggcaacg ctggcctgga cgttgggttt tgaataccac
     2941 cacggcttgc tggagaaaat gtggaaagag atcttaaaaa atcatgccca cgacagcatc
     3001 ggctgctgct gtagtgacaa agttcatcgc gaaatcgtcg cccgcttcga actggctgaa
     3061 gacatggcgg ataatctgct gcgtttctac atgcgcaaaa ttgccgataa catgccacag
     3121 agtgatgccg acaaacttgt gctgtttaac ctgatgccct ggccgcgtga agaagttatt
     3181 aacaccactg tgcggctgcg cgccagccag tttaatctac gagacgatcg cggtcagcct
     3241 gtaccgtatt ttattcgcca tgcccgtgag atcgatccag gcttaatcga tcggcaaatc
     3301 gttcattacg gtaattacga tccctttatg gagtttgata ttcagatcaa ccagattgtc
     3361 ccttctatgg gctatcgaac gctttatatc gaagcgaatc agcctggcaa cgtgattgcg
     3421 gcaaaaagtg acgctgaagg gatactggaa aatgctttct ggcaaattgc gctcaatgag
     3481 gatggaacgc tacaactggt agataaagac agcggtgtgc gctatgaccg ggtattgcaa
     3541 attgaagaaa gctctgatga tggtgatgaa tatgactatt cacccgcaaa agaagagtgg
     3601 gtaattactt cggcgaatgc gaaaccgcaa tgcgagatta ttcatgaggc ctggcagagc
     3661 agggctgtta tccgctatga aatggcggtg ccgcgtaatc tgcaagaacg cagcgcccgg
     3721 caatccacgg gtagagtagg ggtggagttg gttgtcactc ttagtcataa cagcaggcgc
     3781 attgatgtgg atatcaacct tgataaccag gctgacgatc atcgccttcg cgtcctgatc
     3841 cctacatctt ttaacaccga tagtgttctg gcagataccc agtttggttc gctaacgcgc
     3901 cccgtgaacg acagtgcaat gaacaactgg cagcaagaag gctggaaaga agcgccggtt
     3961 ccggtatgga atatgctcaa ctatgcagcc ttacaggaag ggcgtaacgg catggctgtc
     4021 tttagcgaag ggctacgtga atttgaagtc atcggtgaag agaagaaaac ctttgccatt
     4081 acgttgctgc gtggcgtggg cttactgggc aaagaagatc tgcttttaag gcctggacgt
     4141 ccttcaggca taaaaatgcc ggtgcccgat tcacaattac gtggctcctt tagctgccgt
     4201 ctgagcttat taagttatac aggtacgcct gcgaccgccg gtgtagccca gcaggcgcgt
     4261 gcatggctga cgccagtaca gtgttacaac aaaatcccat gggatgcgat gaagttaaat
     4321 aaagccggat tcaatgtgcc ggaaagctat agcttgctga aaatgccacc tgtgggatgc
     4381 ctgattagcg ctcttaaaaa agcggaggac cggcaagaag tggtattgcg actgtttaat
     4441 ccggcagaat cgacaacctg tgaggcaact gttgcgttca gtcgtgaggt gatttcatgt
     4501 acgcaaacga tgatggatga aagcatcagc acagcggaaa gagataattt aaaggaactg
     4561 ggggcatttt taccggggca gtcacggaca tttagctacc gccttggctg attattgcgc
     4621 ttaaagtgta tctaaggggg gcaacaattt caggccatct tcggatggcc tgaaattgtt
     4681 gataaacaag tttgttagct tacgcagata ccagatttga cctgcgtgag aaccgcaggc
     4741 catttatcat ttcagttacc ccttaaacgt taaccacggt cgtaatctga ccgcaggctg
     4801 aatcaatccg gcttcttgtt gggcttcgat aaccggagca atatctttat aagcagctgg
     4861 cgcttcttca agacgtcgtt cttcacgcaa tgtgacgcat tgccacggca gcgttgactc
     4921 ttcagtcgcg cgtgagcgca tcgcctgccg acgctggctg cgtcctgctc catgactgca
     4981 tgaccacaac cactccgagt tgccgcaacc taccgcaagc cacgtataat cgcccattga
     5041 accggggatt aatgccagag cgcccgcgtg tgccggtgtt gcacctttgc gatgaatatt
     5101 catttcatgt tcgcgaagga tgatgttatg cggcacatcc accaccagat gtgaatcatc
     5161 ctgatgaaat aaatcggcaa aacatacgcg aaccatttcc gcaatggtca cgcgatttaa
     5221 ccatgcgtaa cgcgccgcca ttcccatcgc ttgtagatat tcctccgcca gtccaccagc
     5281 cagaccatat aacccgcttt gtgggtgttt atgcccggcg ggccattcag cacgggcacg
     5341 gtccatccag cgtcgtccga cgtaaaagcc gacatctcgc gatccactgt ggatcatcac
     5401 cacgatatcg cccgttttca gcccttgttg ccaggccaca tggcgatcga ccacgctatc
     5461 aaccacctga agttcaataa aatgattacc tgaacctggc gtacccagac aaggatcgcg
     5521 gagtaatgcg cggtccgttg ccagtagggc gggtggggca taacgcgaat gactgttatg
     5581 ctcgttaagc ccaatgcagc ggtcaatctc ttgtaccatt cgggggatat tcgtgcgtgt
     5641 ccagagacct gctgatggca aggtcgatac gtaatctgcc ggtccgccat caaaaagcgc
     5701 agtgaacgct tgtgcggata acggaacatc gcgctcattt tgcagtagca cttgcgttaa
     5761 ccgctgagtg atgacgtctt tattcgcttc ggcttgctca agtgttaatc cagtcgttag
     5821 cagccgcatg ccgcaagaaa tatcggtgcc gatggcggcg ggaatgacta aatccggcgt
     5881 tgaggccacc accgcgccaa cggggacgcc attgccgggg tgaaaatctg gcgtcgcgca
     5941 cgtctggcgc acataacctg ggtggtctgg tagcttcact tctgcaaaat caagcagttg
     6001 tgttactgct ttttcttcca gcggtagttc gtccggcagc agtacgcggg cactggcacc
     6061 agatggacga gataatgaaa caatattgtt ggcgcgggaa agttggatcc ctttgcgcag
     6121 aaaggcttta tcaagccttg aaagatgatg aataacgctg tcctgaaata tagacgttaa
     6181 aaaaccccct gtattgcgag ggtaattttg tctttccggt cagcagccag caaaagggaa
     6241 agaaaacgcg cgtagtgtat gtggcgatag caatttttac aagtggtaaa tctggcttat
     6301 tgttaaagag aaattatctt ttgcgaagtc ttttaaggcc atgactcatt caatttaacc
     6361 agacatagat aatatata
//
LOCUS       DAPGOF010000235         6277 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.237, whole genome shotgun sequence.
ACCESSION   DAPGOF010000235 DAPGOF010000000
VERSION     DAPGOF010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.237"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(152..604)
                     /locus_tag="RHV13_004354"
     CDS             complement(152..604)
                     /locus_tag="RHV13_004354"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV1243807.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVTMTSSQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFISRIERYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFTNFYMRYKPQH"
     gene            complement(601..1656)
                     /gene="dinB"
                     /locus_tag="RHV13_004355"
     CDS             complement(601..1656)
                     /gene="dinB"
                     /locus_tag="RHV13_004355"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDV1243808.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(1727..2512)
                     /gene="lafU"
                     /locus_tag="RHV13_004356"
     CDS             complement(1727..2512)
                     /gene="lafU"
                     /locus_tag="RHV13_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HDV1243809.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            2484..4196
                     /locus_tag="RHV13_004357"
                     /pseudo
     CDS             2484..4196
                     /locus_tag="RHV13_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(4383..4880)
                     /gene="rayT"
                     /locus_tag="RHV13_004358"
     CDS             complement(4383..4880)
                     /gene="rayT"
                     /locus_tag="RHV13_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HDV1243810.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTAQFQMLRNAIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVCDWPFSTFHRDVARGLYPIDWAGDITDLNAG
                     ERIIL"
     gene            complement(5056..5805)
                     /locus_tag="RHV13_004359"
     CDS             complement(5056..5805)
                     /locus_tag="RHV13_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDV1243811.1"
                     /translation="MSLPSIPSFVLSGLLLICLPFSSFASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLKASDRRWQA
                     LAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDKGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            6015..6275
                     /gene="dinJ"
                     /locus_tag="RHV13_004360"
     CDS             6015..6275
                     /gene="dinJ"
                     /locus_tag="RHV13_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HDV1243812.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
BASE COUNT         1516 a         1651 c         1624 g         1486 t
ORIGIN      
        1 acgaatccag ggcaagaaaa aaagaattaa tcctggaaag gtctgcaaag agtcattgat
       61 aaaggatttg tcatgattta cctccttgca gtttgtggaa taagaaaacg cgcacatacc
      121 aggcgggcgt tattttcatt gcaagctgga tttaatgttg cggtttatat cgcatataaa
      181 aattagtaaa ccacgctccc cggcattcaa cgcgctgctg cttaactgtc tgaaaaccat
      241 aacgttcaaa aaagggtttt gcggttatgc tggcatctac cgtaagttcg gattcagact
      301 taatcaatgg ttttaacaag gcgctggcaa ccccgcggcg ggtgtattca gggtcaacaa
      361 ataacatatc gatataacgt tcaatgcggg aaataaaacc aaccggttgt gcattaatga
      421 cggcaacccg cacttgtgat ttcgcgagtt tctccttcca gcgagattcg tcaatctgcg
      481 cccaggcggc aatttgttgt ggagaatagt gctgactgga ggtcatcgta accgctctaa
      541 tgaaaatagc gcatagttgc tgaaaatcgc caggctgata gtttcttatt tgtatgttat
      601 tcataatccc agcaccagtt gtctttccat ttgcggatcc agcaacgtca catgcagccc
      661 caccagtcgc acaccgcgcc cgccgcggcg ttcatcccag gttttacgcg cggtggcgat
      721 taagtcagct ttattcagcc gcggccagac gtgctcctgg gtggtttgct gaaaatcgtc
      781 gaactttaat ttcaccccct ggcgagcaat cagtaaatca ggttttacct ttgccagacg
      841 gcgttcaagt tccggataca gccgctcgat aatcgcttca cattcagacc agtgatgaat
      901 atcttccgcc atcgtgcgtt ccacgccgac ggattttcgc aaccgttcgc tgttaacatc
      961 gcgttcgtca atcccctgac tacgctccca caaaatgcgg ccaaatttgc caaagcgttt
     1021 aagcagcatc accagatcac acttttgtac atcaccgcag gtccgcagcc ccatcgcttc
     1081 cagttttgcc gccgagactt tgccgacgcc ggggattttt gccagtggta aggtttgtaa
     1141 aaatgccgga acttctgccg gcgtaatcac aaactggccg ttgggtttat tcatgtcgga
     1201 ggcgattttg gcgagaaact ttactggtgc cacgcccgca gacgccgtca gttgcagctc
     1261 gttgaaaatc gtctggcgga tttcctgggc aatgagggtc gcagaaccgt ggcaatggac
     1321 gctgtcggtg acatcgagat aagcctcatc cagtgacaac ggctcaatgc gagaggtgta
     1381 gcgcgagaag atttcacgga tatgatttga ggcttctttg taggcgtcaa agcgccccgg
     1441 aagcaaggtg agatgtgggc ataatttgag cgccatccct gtcggcatag cgctgcgtac
     1501 gccaaattta cgcgcgggat aattagcggt gctgatcacc ccccgacgtt cgcggctgcc
     1561 gccaatagca atagggatat cgcgcagggc gggattgtcg cgcatctcca ctgcggcgaa
     1621 aaagcagtcc atatccacat gaatgatttt acgcattact cacctctcaa cactggtaaa
     1681 gtatacagtg atttcagggt ttgagaaatg cgtaaagatt cagcatttag cgcgttcgct
     1741 gcgaaagcac ctgctgctta tccagctttt gcaccagcgg ctgcaccact ttatccccat
     1801 gctgaccaaa gtattgatac agcgtatcgg acgcactttt ggtcagcacc ataatctcaa
     1861 tacgccgatt gcccgcgctt tgcggatttt tggcatccag cagcatctgg tcagccattg
     1921 cgcttacctg catcacttta tcttccggca ttccggcctc ttccagcacc cgacgagccg
     1981 aaagcgcgcg gtcacccgaa aggttccagt tgttgtagat attgtttttg taagccatcg
     2041 catcggtatg cccggtaata ataattttgt tatcgagcga gtcgaacact ggcgcgagct
     2101 ccaccagcaa agttttaaag aacggcataa tctgcgcgct gccgcgttcg aacatattgc
     2161 gattctggtc gtctttaatc agcacgcgta atccctgcgg gacaatctcc atctccagat
     2221 tcgcttccat atgcgcatca cgggcaatgg tgttgatact ggttgccagc tcgccaagct
     2281 cggtggcgga tttcttcttc agcagcgccg ttttctcatt cacatcacgc gcttttttct
     2341 ccgtctcttc cggcaccgcc acggtggcag gttttggcgg atgagacggg ctgattttat
     2401 ttaacggcga cagcccacca ccgttaaaaa tcgactgccc gtgcagcgcg gcgataatgc
     2461 tttcacgctc ggatttactg acgctgttga caatcaactt tatcgtcgtc accaaagggg
     2521 ccgagcgtat ttccgaggtt tctgcccgct ttaccctgga cgcgatgccc ggcaaacaga
     2581 tggcgattga cgccgacctt aacgccgggt tgatcaacca ggcgcaggcg caaacccggc
     2641 gtaaagatgt cgccagcgag gccgatttct acggcgcgat ggacggggcg tcaaagtttg
     2701 tgcgcgggga tgccatcgcc gggatgatga ttctggcaat caacctgatc ggcggcgtct
     2761 gtatcgggat cttcaaatac aacctgagcg ccgacgccgc cttccagcaa tatgtgctga
     2821 tgaccatcgg cgatgggctg gtggcgcaga tcccttccct gctgctctcc accgcggcgg
     2881 cgattatcgt cacccgcgtc agcgacaacg gcgatatcgc ccatgacgtg cgccaccagc
     2941 tgctggcaag cccgtcggtg ctctacaccg ctaccgggat catgttcgtg ctggcggtgg
     3001 taccggggat gccgcacctg ccgtttttgc tgttcagcgc cctgcttggt tttaccggct
     3061 ggcggatgag caaacgcccg caggcggcag aggcggaaga gaaaagcctc gaaacgctaa
     3121 cccgcaccat caccgaaacc agcgagcaac aggtcagttg ggaaaccatt ccgctgatcg
     3181 agcccatcag cttaagcctc ggctacaaac tggtggcgct ggtggacaaa gcgcagggca
     3241 acccgctcac ccagcggatt cgcggcgtgc ggcaggtgat ttctgacggc aacggcgtgc
     3301 tgctgccgga gatccgcatt cgggaaaact tccgcctcaa gccgagccag tacgccattt
     3361 tcatcaacgg cattaaggcc gatgaagcgg atattccggc ggataaactg atggccctgc
     3421 cctccagcga aacctacggc gagatagacg gcgtgctggg gaacgacccg gcgtacgggt
     3481 tgccggtgac ctggatccag cctgcgcaga aagcgaaggc gctgaatatg gggtatcagg
     3541 tgatcgacag cgccagcgtg attgccaccc atgtgaataa gattgtacgc agctatattc
     3601 ctgatttgtt taactatgac gacatcacac agttgcataa ccgtttgtcg tcgatggcac
     3661 cgcgcctggc agaagactta agcgcggcgc tgaattacag ccagttgctg aaagtgtacc
     3721 gggcgttact gaccgaaggc gtttccctgc gcgatatcgt caccatcgcc accgtgctgg
     3781 tcgccagtag cgcggtgacc aaagatcata ttctgctggc agccgatgtg cgcctggcgc
     3841 tgcggcgcag cattacccat tcgtttgttc gcaagcagga gctgacggtg tatacgctga
     3901 ataatgagct ggaaaatctg ctgaccaacg tggtgaatca ggcgcaacag ggcgggaaag
     3961 tgatgctcga cagcgtgccg gtcgccccga atatgctcaa ccagttccag aacacgatgc
     4021 cgcaggtaaa agagcagatg aaagcggcgg ggaaagaccc ggtgctgctg gtgccgccgc
     4081 agcttcgccc tttgctggcg cgctatgcga gattgtttgc gccgggactg catgtgctgt
     4141 cgtataatga agtgccggac gagctggagt tgaagattat gggggcgttg atgtaagaat
     4201 ccggcatcgg tgcacaattg ccggatgcgg cgtgaacgcc ttatccggcc taccgttcag
     4261 gcacaggttt ccaggcatga taagacgcgc cagcgtcgca tcaggcatcc gcgcacaact
     4321 gccggatgcg gcacagattt gtaggccgga taagcgtaag cgcatccggc accagcgcac
     4381 aattataaaa taatacgctc gccagcattt aaatccgtta tgtctcccgc ccaatcgatg
     4441 ggatataacc ctcgcgcaac atcgcgatgg aacgttgaga atggccaatc acacacctgc
     4501 ttaacccagc catgtttcac tggatttata taaatataat caacatgatg ccgataatct
     4561 tttgtattac ggatggcatg ctcccaaaaa cgcggttgcc agatattttt caatccgcaa
     4621 gcatgggtga actgcttttt aatctctctc cagcgcgagg aaaaatcatc atcgccttca
     4681 ggtaatgtcc atatgcaatg catatgctct ggtaaaacga cccaagcgtt gatttcaaaa
     4741 ggtctgtctc gcttaacttt aataatagcg ttacggagca tctggaactg agcggtcaga
     4801 aggtggcttc gacgatttcg taggttaacc gtgaaaaacc aggttccccc cttgatgtaa
     4861 taccgacgat attcagacat agcgactcct ttttctcaac ggtaagaaga aaaggaaagg
     4921 tcggcaatga ggtgatggaa taacgggaaa gcggctcgca aaaacgcaca aatttgtagg
     4981 catgataaga cgcgtcagcg tcgcatcagg catccgtgca caaccgccgg atgcggcgta
     5041 aacgccttat ccgtcctaca aaatcgtctc ctccgtcaaa atccgtcgcg cgcctaaaaa
     5101 atgatcctgc cagaaaggtt cagctaaccg acttacccga atggtttccc cggtgcgcgg
     5161 tgactcaata aactgaccgt ctcccagata cacccccata tggtcggcaa tctcacgact
     5221 gtgaatatgg aaaaacagca aatctccccg gcgcaggtcg ttgttcgcca caatcgttgc
     5281 ccgatggtag tgatacatct cattggctgt gcgcgggagc ttagcctcaa ggatcttgtt
     5341 gtaggcataa aaaaccaacc cgctacagtc gaagccttta tcgggccgcg tgccgcccca
     5401 gacgtaaggc ttgccaagtt gctgttccag gcggtgaatc gccacttcgg tgatgttatg
     5461 cagatggtca ctgcttagaa agtggttgtt ttccgccagc gcctgccagc gtctgtcact
     5521 cgccttgagc ggtgcaggaa accattctgg atgctgtttt atctgctccc ggttgtgctg
     5581 gcgtagcgcc cttctgcttt cggcattgcc ttccacaata tagctggcct gttttttcag
     5641 atgggtttgg tactgtttta ataaacgcgc acggttttgc atccgctggc gggcggcgta
     5701 gctgaaagag atatgtgatg tggtggcgct ggcaaatgaa gaaaacggca aacaaatcaa
     5761 cagtaatccc gacaatacaa aggatggaat cgacggcaag gacatactca ataatggctg
     5821 gtcatgcttt aatgttgcag tgtatcccgg atcgtttctg aacaaactta ataaaccatt
     5881 attataaaat taacatatat atttcagtga attatgtaaa tacatcagtt tattgaattg
     5941 ctcatgaatt gccgctgttg ctcaattgag ctacaattca agctgaatat atatacagca
     6001 caggagatac cccaatggct gctaacgcgt ttgttcgcgc ccgaatcgat gaagatctga
     6061 agaatcaggc ggcggacgta ctggccggga tggggctgac catctctgac ctggttcgca
     6121 taaccctcac aaaggtcgcg cgtgaaaagg cattgccgtt tgatttacgc gagcctaatc
     6181 aattaaccat tcaatcaatc aaaaacagcg aagctggcgt tgatgttcat aaggccaaag
     6241 acgccgatga tttatttgat aaattaggaa tttaaat
//
LOCUS       DAPGOF010000236         6216 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.238, whole genome shotgun sequence.
ACCESSION   DAPGOF010000236 DAPGOF010000000
VERSION     DAPGOF010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6216
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.238"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(181..681)
                     /locus_tag="RHV13_004361"
     CDS             complement(181..681)
                     /locus_tag="RHV13_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDV1243813.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDEADPELMFQDYEGFPGNMASECHINWAWVEGFRQARDEGCEEAYRLWVEDTGETD
                     FDTFRDAWWGEADSEEAFAVEFASDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
     gene            complement(1144..1740)
                     /locus_tag="RHV13_004362"
     CDS             complement(1144..1740)
                     /locus_tag="RHV13_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243814.1"
                     /translation="MMKSDEQYQVPVWMRPLLPLLCNTGGNDPEELLNDTETTASANI
                     VRYVLIVAVRSQIDLLQLLYRKGLLRTEIPGGFSPEEAQELLDNLVRSHISKALSGER
                     MAARDRNADLTWIRQQLVDAAWFVRATLEAHGMGVGNESPSAPPETMPDIQTRELVML
                     IKRLASSLKAVKPDSSVVREAQDWLCDRKLVDITDILR"
     gene            complement(1737..2456)
                     /locus_tag="RHV13_004363"
     CDS             complement(1737..2456)
                     /locus_tag="RHV13_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HDV1243815.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYASAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENAI"
     gene            complement(2453..2887)
                     /gene="psiB"
                     /locus_tag="RHV13_004364"
     CDS             complement(2453..2887)
                     /gene="psiB"
                     /locus_tag="RHV13_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HDV1243816.1"
                     /translation="MKTELTLNALQSMNAQEYEDIRAAGSDMRRNLTHEVMREVDAPV
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNGGGQPFSVV
                     QVQHIFTPVAISHTLALAATLDAQGYSVNDIIHILMAEGGQA"
     gene            complement(2942..4900)
                     /locus_tag="RHV13_004365"
     CDS             complement(2942..4900)
                     /locus_tag="RHV13_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDV1243817.1"
                     /translation="MPVTKCEPETTRKASRKSVKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELADSIKGVGLLQNLVVHALPGDRYGVAAGGRRLAALNMLA
                     ERDIIPADWPVRVKIIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAHIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDGDGYVDRV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAED
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEIRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDATDDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFGSGAYSKPAQISLECEHYSLTSDAPSGKEGAAFMAMMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSIHGVQTRECGHTSRSPLDTLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMCAPRPQMDATEHTTNLADAA"
     gene            complement(4959..5192)
                     /locus_tag="RHV13_004366"
     CDS             complement(4959..5192)
                     /locus_tag="RHV13_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HDV1243818.1"
                     /translation="MSEYFRILQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRQDGTLIWRSWNFEDCAGYWMNQYIRDFGILK"
     gene            complement(5250..5777)
                     /locus_tag="RHV13_004367"
     CDS             complement(5250..5777)
                     /locus_tag="RHV13_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDV1243819.1"
                     /translation="MSARGINKVILVGRLGNDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLCKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTVQMLGRAPQQNAQAQPKPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQPQ
                     PPEGEDYGFSDDIPF"
BASE COUNT         1304 a         1931 c         1680 g         1301 t
ORIGIN      
        1 tgtccccgcc tccggaaatc agaacaacag aaacgacata acagaaaaat gcctcacacc
       61 atgacgggca ggaagggcag cgccgcaggc gcggccctcc tgcatttcag gcaggcgtaa
      121 gcctgccagg gttttccggc ggcccggcag gccatacccc cgccggagcg ggggagtaaa
      181 tcaccgacgg aacacatgac cgtcaataaa ggtgaaggag tccaggaata aatcccgcgc
      241 atacgcctca tagtcaaaat agagcgccac cgtctccggc acgtcagcca gcaggccggt
      301 atcactggcg aactcaaccg caaaagcctc ctcactgtca gcctcgcccc accaggcatc
      361 gcggaaggtg tcaaaatccg tctcaccggt atcctccacc cagagacgat aagcctcttc
      421 gcagccttca tcccgtgcct ggcggaagcc ttcaacccag gcccagttga tatggcattc
      481 agaggccata ttccccggga atccctcata atcctgaaac atcagttcag gatcggcttc
      541 atcctggtga agagcacggc aggcggcgaa aaagtcgcgc tcatcatcaa acgtggtcag
      601 gtcaaaccag cgtccggcga tgcttccaca gttgtatttg tgccaggttc caacgtatac
      661 agcaggtgca acaacagaca ttcccgaatc ctccatagtc acatgttctg cggcaccggg
      721 cggtgccgtg cctccataaa agccgggcga taaggcgggg atgcacaggg cgcaagggaa
      781 gccgcagcct gacgcacacg gctggcggga gttggagggt gagaccgcga aatctgagcg
      841 ggtgaacgac cggaaaatca cggcagacgg gaaggaggga ccgggtgacc gcagcggcga
      901 agcgtaccct tgcatccctg ccagcccggc taagatggag gcacggcaca gcccgcacac
      961 gacgctgagg ccacagagct gaaaacccac aaggccatgc cccgcatggc cgggtaaaag
     1021 cggagccgcc ggcgacttcc ttattccggc gagtgcagag gacacgccgc atgacctcca
     1081 gaccacccct gatatgaaac gatcatgatc ggcgtgtccc tgcgtaaaat atacatgtct
     1141 ggttcaccgg agaatatctg tgatatccac aagttttctg tcgcacagcc agtcctgcgc
     1201 ttcacgcacc acgctactgt cgggtttcac cgctttcagc gatgatgcca gtcgcttgat
     1261 caacataacc agttcccgtg tctgtatgtc cggcattgtc tccggcggag cagagggact
     1321 ctcatttccg acgcccatcc catgcgcttc cagtgtggca cggacaaacc aggcggcatc
     1381 gaccagttgc tggcgaatcc aggtcagatc ggcatttctg tcacgggctg ccattcgctc
     1441 acccgacagc gccttgctga tatggctgcg caccagatta tccaggagtt cctgcgcttc
     1501 ttccggtgaa aagccacctg gtatctctgt gcgcaacagt cctttcctgt acagtagctg
     1561 cagcagatca atctgcgacc gcactgcaac tatcagtaca taacggacaa tattcgcact
     1621 ggcggtggtt tctgtatcat tcagcagttc ctccggatca ttaccaccgg tattgcagag
     1681 caacggcaac agaggtcgca tccacacggg aacctgatat tgttcgtctg atttcatcat
     1741 attgcgttct ccagcttgtt cgggatcatc cactgctcaa gtgcacgtac ctgtaccggt
     1801 gcaccacgac cggcctcacc ggcttcaaaa atcagccgcc agagtggttc ctcctgccag
     1861 aaaaaccggt caaatgatga cagtgatgga aaacgcccgc accaccccca gaaaatcgtt
     1921 tccagatcat gctcctcaac aacaccagac cagcgggaca accagcttcc gaccgtggcg
     1981 ggagtatgaa acgcaaggtc aatctgcgcc tgagcaaaac atgtctggta ttccagttcc
     2041 cgtcgttctg cctcacgttc ccgctggcgg ctgtatttct gctctgtcag cgtggttttc
     2101 tgacgacgcc gctcaccttc acgaaaagga acggacggga acagacgatg ccccgcatcg
     2161 gatggcaggg gaagaacaca gacttcacca aaactttcaa tcagtttatc cacggcagcc
     2221 agccacagga gacggttgcc atgaaattcc tccttcgtca gcgcaggggc aaaaaagcgg
     2281 agatcggtca gcgaaatccg gcgactgcca ttcaggcagc gaaagaaagc gctggcataa
     2341 ggccatgctg aaagtgtgcg tccctgacga cgacgttgtt cggtgacagc cacagcctgc
     2401 atggcggcct gttgttctgc gctgaggggg atcagtgccc gtgaacgtgc gctcatgcct
     2461 gacctccttc tgccatcaaa atatgaatga tgtcgttaac actgtatccc tgcgcatcaa
     2521 gtgtcgcggc gagcgccagc gtgtgactga tcgcgacagg cgtgaagata tgttgcacct
     2581 gaacgacgga gaaaggctgt ccaccaccat tcaccagaac cagcatccag agttgagaga
     2641 cgtctcccgg cgaacataat gccaggtgga aacgttcgtg ggctggcgtg aaacgaacct
     2701 ggacggggaa aaagcccccg aactcactgc catactcgcc attcatcatc cagttaaccg
     2761 gtgcgtccac ttcacgcatc acctcgtgag tgagattacg gcgcatatcg cttcccgcag
     2821 cacggatatc ttcatattcc tgtgcgttca tggactgcag ggcattcagg gtcagttcag
     2881 ttttcatcac ggtatctccc ttgctgccgc cccgtctccg gcggggcggt gtggttgttc
     2941 atcaggcggc atcagccagg ttagtggtgt gttcagtggc atccatctgt ggacgtggtg
     3001 cacacatcca gccaggcacc cagcggttgt ctttcatatg gaactctgca tgttcagccg
     3061 catcgccttt cttcatcttc tccgcgtccc gtgcggcacc ggacagtcct gcctcattca
     3121 gggctgcgat aatctgcggc tttttcaggt gtccgaagaa gtttgctttt gtcggctgcc
     3181 accagtcgcg catgtgaaaa ccgatggcgc tctccagcgt gtccagcggg ctgcgtgacg
     3241 tgtgaccaca ctcgcgggtc tgaacgccgt ggatgctgca cgcggtgcag aaactgagca
     3301 gggataacag cacctcctga ctgagtgaca ggaacgtcgt catgtcccgt gaccatccct
     3361 ccggtaacag ggccgcaaga cgggcttttt ctgccatcat cgccatgaat gcggcacctt
     3421 ccttccctga cggcgcatcg ctggtcagcg aataatgttc acattccagg ctgatttgtg
     3481 ctggtttact gtacgccccg ctgccaaaca cattcaggca gagcgtccat gccagcagtg
     3541 ccagagattt gtccggctgc tgcatgagtg ccgcctggac tgccagcgtg cgctctgagg
     3601 acattttcgt cagcaacggc aggctgattt cctccactga cgctttctcc tgcacctgtt
     3661 ccgtgcggtc agcgtcgtca gtcgcgtcat cttcactgcg caactgcaca ccacgctgga
     3721 cacatacgtt gccataacgc caggacacca ccacaccgct tccggcacgt atctccggcg
     3781 tccacgctct gaccttcgcc atgcagcgca tcagcttcat ttctgcttcc agcaggtcgg
     3841 attcctcaca ctggttttcc agcgcgtcgt aacgcgtcat cagttcgttc aggcgttcgt
     3901 cttccgcctc cgtcagcacc gcttccggct ccggcagaca gcggtatgtt ccggcatcct
     3961 cacggcactc accgacaggc tccatgcgcc cggcgcacca ttcccagcct tcggcttccc
     4021 gaaggtgttc agcgacagcc tggagttttt ccagcagggc ggcatcgagc gccacgcggt
     4081 cgacataacc gtccccgtca tcgctgaaca aatcggtgcg cagttcgtct ggcgagaagg
     4141 catcagcccc cacgaagcgg aatttactgt tcccagccac cgccacttca ctttcggtca
     4201 ccagacgacg aatggtctgt acttccggtt taccgcccca tcccgactga caggcggctt
     4261 caaacacctg cacctgacgc gcggtgtcgt tctccagcgc cagcgcctga cagtgttcgg
     4321 tggtgatgcg gtcttctgcc agcgcatcga ggatgacagg cgcaaggtca gccagtttca
     4381 gcattcgctg aacgtggcgg ggtgaatagc ccagcaaatc accgatgtgt gcaggtgttt
     4441 tgccttcctg cgccattgcg cggaatccgg caatctgttc ggcagggtgc atatcccgac
     4501 gatgaccgtt ctcggtcatt gatgcggcag tcgccagctc ctgcggaata atttttacgc
     4561 ggacaggcca gtcagccgga atgatgtcac gctctgccag catgttgagt gctgccagtc
     4621 ggcgaccacc tgcggcgaca ccgtaacggt cacctggcag ggcatgaaca accagattct
     4681 gcagcaggcc gactccctta atggaatcag ccagttcact gacggactcc gcagaatacg
     4741 gcaccgtgcg cacattcagc ggtgatttaa tcagcgaggc cagcggcacg ctcacttcct
     4801 ccgtctgcgc cagcagggca gacaggacag tttcctgcgt ttttacagat ttacggcttg
     4861 ctttgcgggt ggtttctggt tcacacttcg ttacaggcat aatcatcccc ttataattgc
     4921 ttatgtcctg ccggacgttc gcgcgtccgg cacctctctt acttaagaat cccgaaatcc
     4981 ctgatgtact ggttcatcca gtacccggca cagtcctcaa aattccagga gcgccagatc
     5041 aacgtgccat cctgacggac aaccaggcga aaatgcgttc cctgatcatc ctcgataaag
     5101 acgttccggt actgtgcggc aacggcttcc gcctgttcgc gggtaaagga gccgtccggc
     5161 agtccctgaa gtattctgaa atattcgctc atcctcatat ctccggtgat gccccgcaca
     5221 gggcggggcg gttgtcacag tcagcccgat cagaacggga tgtcgtctga aaacccgtaa
     5281 tcctccccct ccggcggttg cggctgaggc tctggctgcg cggccttacg tccacggcct
     5341 ttcgttttcg cgccaccttt tttcgtcgcg tcagcactct gtggctgccc attctgctga
     5401 ggcttcggtt gcgcctgagc gttctgctgt ggtgcacgtc ccagcatctg cacggtgccc
     5461 gtggtcttaa caagaatttc agtgacgtaa cgggtgatac cgttatcttc ccagctacgg
     5521 gtgcgaagct gaccttcgat gtagacctgc gcacccttgc acagatattc acctgccact
     5581 tccgcgagct tgccgaacag caccacgcgg tgccattccg tctgctcccg catctccccc
     5641 gtctgtttgt cacgccagct ttctgatgtg gccacctgca ggtttgccac tgcgcccccg
     5701 ttggggatgt aacggacttc cggatcattg cccagacgcc cgacgaggat gaccttgttg
     5761 ataccacgtg cactcataag aacctcctga ctgaaagatg tgagataaac cggataccgg
     5821 cgacgggacg ttcatgtgcc ccttcagagg agcgaaagct cctcctgccg tccacttcac
     5881 aggggtttct ccccgcagtg gacctttccg atgggggccc gtttaccgcc tcacgggaca
     5941 gctgtccggg ggcggacgca aaatttttag ccgggttcat ggctaaaaat tttgtggccg
     6001 gtgcatctca ccctggacag atggaacggg agggggttac gtctgccccc cggaaagtcc
     6061 acagcgggaa acaggccgca ggggtggcgg caggagggaa gactgctgcg ccatcaggcg
     6121 cagtttcctc acacaccgtc aggtgtgcag ggagcgcagg tcacggcgcg acaggggcgg
     6181 cgcgaccgga acggtcgctg caagccgggt tttcac
//
LOCUS       DAPGOF010000237         6114 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.239, whole genome shotgun sequence.
ACCESSION   DAPGOF010000237 DAPGOF010000000
VERSION     DAPGOF010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6114
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.239"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            276..1388
                     /locus_tag="RHV13_004368"
     CDS             276..1388
                     /locus_tag="RHV13_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HDV1243820.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNDKVDGDKTYMRLGFKGETQVTDQLTGYGQWEYQIQGNAPESENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGRDALEQNGDGVGGSITYDIGEGFSFGGAISSS
                     KRTAEQNNSARFIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATLAGDLGWANKAQ
                     NFEVVAQYQFDFGLRPSVAYLQSKGKNLNANIAGRTYDDEDLVKYVDVGATYYFNKNM
                     STYVDYKINLLDDNNFTRAAGINTDDIVALGLVYQF"
     gene            1500..2555
                     /gene="apbE"
                     /locus_tag="RHV13_004369"
     CDS             1500..2555
                     /gene="apbE"
                     /locus_tag="RHV13_004369"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HDV1243821.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MAEIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            2629..3693
                     /gene="ada"
                     /locus_tag="RHV13_004370"
     CDS             2629..3693
                     /gene="ada"
                     /locus_tag="RHV13_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HDV1243822.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFYLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCALGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRDDGSLSGY
                     RWGASRKAQLLRREAENEER"
     gene            3693..4343
                     /gene="alkB"
                     /locus_tag="RHV13_004371"
     CDS             3693..4343
                     /gene="alkB"
                     /locus_tag="RHV13_004371"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HDV1243823.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            4419..6062
                     /gene="yojI"
                     /locus_tag="RHV13_004372"
     CDS             4419..6062
                     /gene="yojI"
                     /locus_tag="RHV13_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HDV1243824.1"
                     /translation="MELLILVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFLLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANSQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         1451 a         1519 c         1655 g         1489 t
ORIGIN      
        1 taagaataag ttattgattt taaccttgaa ttattattgc ttgatgttgg gtgcttattt
       61 cgccattccg taataatctt aaaaagttcc cttgcattta cattttgaaa catctatagc
      121 gataaatgaa acatcttaaa agttttagta tcatattcgt gttggattat tctgcatttt
      181 tggggagaat ggacttgccg actgattaat gagggttaat cagtatgcag tggcataaaa
      241 aagcaaataa aggcatataa cagagggtta ataacatgaa agttaaagta ctgtccctcc
      301 tggtcccagc tctgctggta gcaggcgcag caaacgctgc tgaagtttac aacaaagacg
      361 gcaacaaatt agatctgtac ggtaaagtag acggcctgca ctatttctcc gacaacgata
      421 aggttgatgg cgacaagacc tacatgcgtc ttggcttcaa aggtgaaact caggttactg
      481 accagctgac cggttacggc cagtgggaat atcagatcca gggcaacgct ccggaaagcg
      541 aaaacaactc ctggacccgt gtggcatttg ctggtctgaa attccaggac gttggttctt
      601 tcgactacgg tcgtaactac ggcgttgttt acgacgtaac ctcttggacc gacgtgctgc
      661 cagaattcgg tggcgacacc tacggttctg acaacttcat gcagcagcgt ggtaacggct
      721 tcgcgaccta ccgtaacacc gacttcttcg gtctggttga cggcctgaac tttgctgttc
      781 agtaccaggg caaaaacggt agcgtaagcg gcgaaggcat gaccaacaac ggtcgtgatg
      841 ctcttgagca gaacggtgac ggtgttggtg gttctatcac ttatgacatc ggtgaaggct
      901 tcagcttcgg tggtgcgatc tccagctcca aacgcaccgc agagcagaac aacagtgctc
      961 gtttcatcgg taatggtgat cgtgcagaaa cctacactgg tggtctgaaa tacgacgcta
     1021 acaacatcta cctggctgct cagtacaccc agacttataa cgcaactctg gctggtgatt
     1081 taggctgggc gaacaaagcc cagaactttg aagtggttgc ccagtatcag ttcgacttcg
     1141 gtctgcgtcc ttctgttgct tacctgcagt ccaaaggtaa aaatctgaat gccaacatcg
     1201 ctggtcgtac ttacgacgac gaagatctgg tgaaatatgt tgatgttggt gctacctact
     1261 acttcaacaa aaacatgtcc acctatgttg attacaaaat caacctgctg gacgacaata
     1321 acttcacccg cgctgctggc atcaacactg atgacatcgt agctctgggt ctggtttacc
     1381 agttctaatc tcgattgata tcgaacaaag ggcctgcggg cccttttttc attgttttca
     1441 gcgtacaaac tcagtttttt ggtgtactct tgcgaccgtt cgcatgagga taatcacgta
     1501 tggaaataag ctttacccgc gtggcactgc tggctgccgc gctcttcttt gttggttgcg
     1561 atcaaaaacc acaacccgcc aaaacccacg ctactgaagt taccgttctt gaaggcaaaa
     1621 ccatgggtac cttctggcgt gccagcatcc cgggcattga tgccaaacgc agtgccgaac
     1681 ttaaagaaaa gattcagacc cagctggacg ccgacgatca gctgctttcg acctataaaa
     1741 aagattccgc gctgatgcgc tttaacgact cgcaaagttt gtcgccgtgg ccagtaagtg
     1801 aagcgatggc cgaaatcgtc actacctcgc tgcgcattgg cgcgaagacc gatggtgcga
     1861 tggatattac cgtcgggccg ctggtgaatc tgtggggctt tggcccggaa caacagccgg
     1921 ttcaaattcc gagccaggaa cagatcgatg cgatgaaagc caaaaccggc ttacagcacc
     1981 tgacggtcat taatcagtcg catcagcaat atctgcaaaa agacctgccg gatttatatg
     2041 tcgatctctc taccgtcggc gaaggttatg cggcggatca cctggcacgc ttgatggagc
     2101 aggaagggat ttcccgctat ctggtgtcgg tgggcggcgc gctgaacagc cgtggtatga
     2161 acggtgaagg cctgccgtgg cgggtagcga ttcaaaaacc aaccgataaa gaaaacgcag
     2221 ttcaggctgt ggtggatatc aacggtcatg ggattagcac ctctggcagc taccgtaact
     2281 attacgaact ggacggcaaa cgtctttccc atgttatcga tccgcaaacc gggcgtccca
     2341 tcgaacacaa tctggtatcc gtgacggtga ttgctccgac ggcgctggaa gccgatgcct
     2401 gggatactgg cttgatggta ctcgggccgg agaaagccaa agaggttgtt cgccgggaag
     2461 ggctggcggt ttatatgatc accaaagaag gcgatagctt taaaacctgg atgtcaccac
     2521 agtttaaaag cttccttgtc agcgaaaaaa attaaagcgc aagattgttg gtttttgcgt
     2581 gatggtgacc gggcagacta aaggctatcc ttaaccaggg agctgattat gaaaaacgcc
     2641 acatgcttaa ctgacgatca acgctggcaa tctgtcttag cccgcgaccc gaatgccgac
     2701 ggcgaattcg ttttcgccgt gcgcaccaca ggcatctttt gccgtccgtc ttgccgcgcc
     2761 agacatgcct tgcgggaaaa tgtctccttc tacgcaaatg ccagcgaggc actcgccgcc
     2821 ggatttcgcc cctgcaaacg ttgtcagcca gacaaagcca atgcccagca acatcggttg
     2881 gataaaatca cccacgcgtg tcgactgctg gaacaggaaa cgcctgtaac gttggaagcc
     2941 ttagccgacc aggtggcgat gagtccattt tatctacatc ggttgtttaa agcgactacc
     3001 ggaatgacgc ctaaagcctg gcaacaggcc tggcgcgctc gccgtttgcg cgaatcgctg
     3061 gcgaaagggg agagcgtgac gacgtctatt cttaacgccg gattccccga cagcagcagt
     3121 tactaccgca aagccgatga aacgctgggc atgacggcta aacaattccg tcacggtggc
     3181 gaaaatctgg cggtgcgtta cgcgctggct gattgtgcgc tggggcgttg cctggtggca
     3241 gaaagcgagc gggggatttg cgcgatattg ctgggcgatg atgacgcgac actaatcagc
     3301 gagttgcagc agatgtttcc cgctgccgac aacgcgcctg ccgatctgac gtttcagcaa
     3361 catgtgcgtg aagtgatcgc cagcctcaat caacgcgata cgccgctgac gttaccgctg
     3421 gatattcgcg gcactgcttt tcagcaacaa gtctggcagg cactgcgcac gataccttgc
     3481 ggtgaaaccg tcagttatca gcaactggct aacgccatcg gcaaaccgaa agcggtacgg
     3541 gccgttgcaa gtgcctgtgc cgccaacaag ctggctatcg ttataccctg tcatcgggta
     3601 gtacgcgatg atggatcgct ttcaggttac cgctggggcg cgtcgcgcaa agcgcaactg
     3661 ctgcgccgcg aagctgagaa tgaggagagg taatgttgga tctgtttgcc gatgctgaac
     3721 cgtggcaaga gccactggcg gctggtgcgg taattttacg gcgttttgct tttaatgctg
     3781 cggagcaact gatccgcgat attaatgacg ttgccagcca gtcgccgttt cgccagatgg
     3841 tcacccccgg gggatatacc atgtcggtgg cgatgaccaa ctgtgggcat ctgggctgga
     3901 cgactcatcg gcaaggttat ctctattcgc ccattgatcc gcaaacaaat aaaccgtggc
     3961 ccgccatgcc tcagagtttt cataatttat gtcaacgtgc ggctacggcg gctggctatc
     4021 cagatttcca gccagatgct tgtcttatca accgctacgc tcctggcgcg aaactgtcgc
     4081 tgcatcagga taaagacgaa ccggatctgc gcgcgccaat tgtttctgtt tctctgggct
     4141 tacccgcgat ttttcaattt ggtggcctga aacgtaatga tccgctcaaa cgtttgttgt
     4201 tggaacatgg cgatgtggtg gtatggggcg gtgaatcgcg gctgttttat cacggtattc
     4261 aaccgttgaa agcggggttt catccactca ccaccgactg ccgctacaac ctgacattcc
     4321 gtcaggcagg taaaaaagaa taaaaataag aattattatt gctgtgcgcg cgaagatgtt
     4381 taaactgcgg gctgtaattc attgtccggg ttttctgcat ggaacttctt atacttgtct
     4441 ggcggcagta tcgctggcca tttatcagtg tgatggcgct aagcctcgcc agtgcggcat
     4501 taggcattgg cttaattgct tttatcaatc agcgccttat cgaaacggcg gataccagtc
     4561 tgctggtgtt gccggagttt ctgggattat tgctgctgtt gatggcagtc actctcggat
     4621 cgcaactggc gctcaccact ttggggcatc acttcgttta ccgactgcgt agtgaattta
     4681 tcaagcggat tctggatact cacgtcgagc gcattgaaca actcggtagc gcctcgttgc
     4741 tggcgggatt aaccagcgat gtgcgcaata tcaccattgc ttttgtgcgt ctgccggaac
     4801 ttgtgcaggg gatcattctc actatcggtt ccgcggcgta tctgtggatg ctgtcgggca
     4861 aaatgttgct ggtaacggct atctggatgg cgatcaccat ctggggcggt tttttgctgg
     4921 tggcgcgggt gtacaaacat atggcgaccc tgcgtgaaac cgaagacaag ctgtacacgg
     4981 attttcaaac tgtacttgaa gggcgcaaag agctgactct gaaccgggaa cgcgccgagt
     5041 atgtgtttaa caacctctac attcctgatg cgcaagagta tcgccatcat attattcgcg
     5101 cagacacctt ccatcttagt gccgtgaact ggtcaaacat catgatgctg ggcgcaatcg
     5161 gcctggtgtt ctggatggcg aacagcctcg gttgggctga taccaacgtt gccgcgacct
     5221 attcgttgac gcttttattc ctgcgtacgc cgctgctttc ggcggttggc gcattgccga
     5281 cgctgctgac ggcgcaggtg gcgtttaaca agctgaacaa attcgcgctc gcgcctttca
     5341 aagcagagtt tccgcgcccg caggcgtttc ccaactggca aacgctggag ctgcgtaacg
     5401 tgacgtttag ctatcaggat aacgcgtttt ccgttgggcc gattaacctc accattaaac
     5461 gtggcgagct gctgtttctg attggtggca acggtagtgg aaaatcgacg ctggcgatgt
     5521 tgttgacggg cttgtatcag ccacaaagcg gcgaaatctt gctggatggc aaacctgtca
     5581 gcggcgaaca accggaagat tatcgcaaac tgttttcggc agtgtttacc gatgtctggc
     5641 tgtttgatca actgctgggg ccggagggta aacccgctaa ctcgcaactg gttgagaagt
     5701 ggctggcgca gctgaaaatg gctcataagc ttgagttaag caacgggcgt attgttaacc
     5761 tgaagttatc aaaagggcag aaaaaacgcg tggcgctgtt gctggcgctg gcagaagaac
     5821 gcgatattat cctgctggat gaatgggcgg cggatcagga tccacacttc cgtcgtgagt
     5881 tttatcaggt gttgctgccg ctgatgcagg agatgggtaa aactattttc gccatcagtc
     5941 atgatgatca ttactttatc cacgccgacc gcctgctgga aatgcgcaac gggcaactta
     6001 gcgagctgac gggcgaagag cgcgatgctg cttcgcgtga tgccgttgcc cggacggcat
     6061 aatattacga cgctgacctg gcggactgct gccgccaggt caatatgcta aaaa
//
LOCUS       DAPGOF010000238         6076 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.241, whole genome shotgun sequence.
ACCESSION   DAPGOF010000238 DAPGOF010000000
VERSION     DAPGOF010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.241"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(188..823)
                     /gene="msrQ"
                     /locus_tag="RHV13_004373"
     CDS             complement(188..823)
                     /gene="msrQ"
                     /locus_tag="RHV13_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HDV1243825.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     SPIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            complement(824..1828)
                     /gene="msrP"
                     /locus_tag="RHV13_004374"
     CDS             complement(824..1828)
                     /gene="msrP"
                     /locus_tag="RHV13_004374"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HDV1243826.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKALEFSKPAAWQNNLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFVGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(1937..2350)
                     /gene="hiuH"
                     /locus_tag="RHV13_004375"
     CDS             complement(1937..2350)
                     /gene="hiuH"
                     /locus_tag="RHV13_004375"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HDV1243827.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2483..3154
                     /gene="hprR"
                     /locus_tag="RHV13_004376"
     CDS             2483..3154
                     /gene="hprR"
                     /locus_tag="RHV13_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HDV1243828.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3154..4512
                     /gene="hprS"
                     /locus_tag="RHV13_004377"
     CDS             3154..4512
                     /gene="hprS"
                     /locus_tag="RHV13_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HDV1243829.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLANELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDSINKIVNRTNVSDGMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNSYLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRITLPQ
                     RN"
     gene            complement(4609..5460)
                     /gene="hchA"
                     /locus_tag="RHV13_004378"
     CDS             complement(4609..5460)
                     /gene="hchA"
                     /locus_tag="RHV13_004378"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HDV1243830.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKLFTGDSPFAANALGKLAAQE
                     MLAAYAG"
BASE COUNT         1672 a         1281 c         1471 g         1652 t
ORIGIN      
        1 actcttagga aacgagagca tggctttctc agttctactt atctatctta ttgaaataaa
       61 ataaattatt tttgtgattt gaggttgatg acaaacgaaa cataacctga tgtgatctgc
      121 gtaggccgga tagggcgctc gcgccacatc cggcagggat aacgcgtact ttgtcggcaa
      181 tatgaagtta tacagataat ttgttgtgaa cctgcttgcg taaccggtta aaaagggaac
      241 gcaacttctt ataccgtaag gctaaaagca gtacagccag cccagcgtag atgagcggct
      301 gcggtgaaat aatcttcact gaccacagat aatgtattgg ggacaggatc gcgacaagat
      361 agacgaaatt atgcaactgt tgccagtgct tgccgagttt tcgctgcatc gcctgggttg
      421 aagtaaacgc taaagcaagc aaaataaccc agctgataat tcctaacgtt aaataaggcc
      481 gggtaattaa ctccttgcct aataacgcca gattattcac gcccagctcc agcaatgcgt
      541 aactggttaa atgcagtgtc gcccaggcaa agcaccataa gcctaacagg cggcgagtgc
      601 gtatcaataa cggctgtttt gcgtagcgtg ccagaggtgt gattaacaag gtcgccagca
      661 aaaatttcag tgcagtgcga ccagtaaaat gctgaatatc tttcaccgga tcggcaccca
      721 gtccaccgtg attgattgcc cagaccagcc agagaaacgg caacaatccg gcaagatgca
      781 ggcaaacttt cagccatgtc acctgttttg ctgtcagacg catttagaaa ttctcccgca
      841 aatccaggcc acgatacagc gatgccacct ggtcggcgta accattaaac agtagcgttg
      901 gctggcgctg tacatcgagg atgccgcctg aaccaacaaa tcgttcggta gcctgtgacc
      961 agcgcgggtg atcaacatgc ggattaacgt tggcgtaaaa accgtattcg tcaggcgctg
     1021 ccagattcca ggtggttggc ggacgctcgc gggtcagctt aatactgact atcgatttaa
     1081 tccctttaaa gccatatttc cacggcacaa tcagtcgcac cggcgcgcca ttttgtggcg
     1141 gtaacgcctt gccataaaca cctacggtca tcagtgtgag cggatgcatt gcttcgtcga
     1201 gacgcaatcc ttcgacataa ggatatttca gcccgccgcc gataaagcgg tcctgctggc
     1261 ctggcatctg ttccggtgca taaattgttt cgaaagcgac atacttcgca ttgctggtgg
     1321 gttcggcaag cgccagcaat ttgtgcagcg gaaaaccaat ccacggcacc accatcgacc
     1381 acgcttccac gcagcgcata cgataaatac gctcttccag cgggaagcga cgggttaaat
     1441 catcgtgatc gagggtcaat ggttttgcca cttcgccgct gattttcagt gtccatggat
     1501 cggttttcag gctaccagca ttagcggcgg gatcggcttt atccagcccg aattcataga
     1561 agttgttata accggagact ttatctgctg gcgtcagtgg caggttattt tgccaggcgg
     1621 caggcttgct gaactccagc gcttttccgg cgggggcggg tgggcgatcg ttccctttaa
     1681 accagctaag cagatcggca tgcgcagcgt gaggcaaaga aagtgcagct gcgctgatgc
     1741 ccagtgcttt taacacctgc cgacgcttca taaagaatac cgactcggcc gtaacatctg
     1801 attcttttaa aaattgattc tttttcataa gccatcacac ttttttgtcg ttagccagca
     1861 agagccgttt actgctaatc tgtcagggag tgtcctccct aacagtagca gagatctgct
     1921 tttacatatt tatgcattaa ctgccacgat aggttgaata cccatattgg ctaagtaata
     1981 aaggcacatg ataatgctcg ttcactttat taatatgaaa ttcaacgggg atctcaggga
     2041 agaaactttc aagattttgt ttcttgaaat agtccccggt tttaaatacg acacggtaat
     2101 cgcccgtagt tgcagtttgc tcgggccaca gtgccttaat tcgtccatcc ttatctgttt
     2161 tggcggtatt aagttgtaac cagccgttat ccgctttctt ttcaagagtg actgtcacgt
     2221 cggcagcggg ttttcctgtt tgctggttca aaatgtgcac gctaagaatg ttttgttgtg
     2281 cagcatagac caaagaaggt aatgaaaatg cagccgttac tacggagagt actaaataac
     2341 gctttaacat gtttatatcc ttgtcatgtg aatgagtttt gaggatagcg aaaaagcgtc
     2401 cagggtaacc ttacagcagc attacaattt tgtaatgaac caggctgttt ctataacata
     2461 tgatttatgg catattattt tcatgaagat tttacttatt gaagataatc aaaggaccca
     2521 ggagtgggta acgcaggggc tttccgaagc gggttatgtc atcgatgccg tttctgatgg
     2581 cagagatggg ctttatcttg cgctgaagga tgattatgca ttgatcattc tggatattat
     2641 gcttccgggt atggatggct ggcagatctt acaaacgtta agaacggcaa agcaaacccc
     2701 tgttatttgc cttactgcaa gggattctgt cgatgacaga gtcagagggc tggacagtgg
     2761 agcaaatgat tatctggtaa aacctttttc attttctgag ttgctggcaa gggttcgggc
     2821 acaattaagg caacatcacg ctttgaattc aacattagaa atcagcggct taagaatgga
     2881 ctctgttagt caaagtgtga gcagggacaa tatcagtatt acactgacgc gcaaggagtt
     2941 tcagttactt tggctactgg cctccagagc tggcgaaatt atacccagaa cggttattgc
     3001 gagtgaaatt tggggaatca actttgatag tgataccaat acggtggacg tcgccattcg
     3061 caggctccgc gcaaaagttg atgatccttt tcctgaaaag ctaatcgcca caatccgggg
     3121 gatgggctat tcattcgtag cggtaaaaaa ataatgaaaa gattatctat aaccgtccgt
     3181 ttaaccttgc tttttatatt gctgctgtct gttgctggcg ctggaattgt atggactctc
     3241 tataatggcc tggcaaatga gttgaaatgg cgcgatgata caacactcat taaccggaca
     3301 gcacagatca agcagttgtt aattgatggg gtaaatccag atacgttacc tgtgtacttt
     3361 aaccggatga tggatgttag tcaggatatc ttgatcattc atggtgatag catcaataaa
     3421 attgttaacc ggacaaatgt cagtgatggc atgttaaata acatacctgc tagtgagaca
     3481 atcagcgcag ctggcattta cagaagcatt attaatgata cagagataga tgctttacga
     3541 attaatattg atgaagtttc gccatcatta acggttactg tggctaaatt ggcttcagcc
     3601 agacataaca tgcttgaaca gtataaaatc aatagcatta taatttgcat tgtcgccatt
     3661 atactgtgct cagtattaag tccgctgtta atccggacgg ggttacgaga gatcaaaaag
     3721 ttgagtggtg taacggaagc gctgaattat aacgatagcc gagagcctgt tgaggttagc
     3781 gcattaccga gagaactaaa acctcttggg caggcgttga ataaaatgca tcatgcttta
     3841 gtcaaagatt ttgagcgtct aagtcagttt gctgacgatc tcgctcatga acttagaacg
     3901 ccaattaatg cattactggg tcagaatcag gttacgctca gtcaaaccag aagtatcgct
     3961 gaatatcaaa aaacaattgc cggaaacatt gaagagctgg aaaatatttc gcggttaaca
     4021 gagaacatac tgtttcttgc cagggcagat aaaaacaatg ttttggtgaa actggactcg
     4081 ctttctctca ataaggaagt cgaaaatttg ttggactatc ttgaatacct ttcagacgag
     4141 aaagagattt gctttaaggt cgagtgcaat cagcaaatct ttgcggataa aattttacta
     4201 caacgaatgt tatcgaatct tattgttaac gctattcgat attcgccaga aaaatcgcgt
     4261 attcatataa ccagttttct tgataccaac agctatctta atattgatat cgccagccct
     4321 ggaacgaaaa ttaatgagcc tgaaaaactc ttccgtagat tttggcgggg agataattcg
     4381 cgtcattccg taggtcaggg actaggcctt tctttagtca aagcgattgc cgaattacat
     4441 gggggaagtg ctacgtatca ctatctcaat aagcataatg tgttccggat tacgttgccg
     4501 caaagaaatt aacataggct tcagtacgcc cgttaaaaaa cgcagccacg tcgtgagtac
     4561 taacgcggct gcgattgatt atgcgcttac attcaaacgt aacagggatt aacccgcgta
     4621 agctgccagc atttcctgcg ccgccagttt acccaacgca ttcgctgcaa aaggactgtc
     4681 gccggtgaaa agtttacggt ccttatgtac tcgcccggtg atgtcgtcat taatgatgtt
     4741 catgcccatt ttcttcagct cttcgccgaa gtaccaggtg agatgacccg gcatatagcc
     4801 aatctctggc gtttgtttgt ctgcggcgtc tgggaatgcg caaatggaat aaccattcag
     4861 tgggttatcg ccgtggcgaa gcgccagaaa agccgccggg ccgtggcaaa gggagataac
     4921 aaaacggtca tttttgattg cccactgtaa agcggcagct acgtcctggc tttcaggtaa
     4981 accaataagt gcgccatgac caccaggaac aaagattgct gcatattcgc tatcagcgtt
     5041 gaggctggcg acaacatccg cgagtttctt cggattgcgg aacaacgatt tatgctgctc
     5101 aaagaatggc attacttttt catctttgtg cggcatagcc cagtattcaa acttggtcat
     5161 cagaccggaa atggtcgcca cttcgaattc gaaacctgca gcatggagat gatacaacgg
     5221 cagcaacgtt tcaatcggat ggttaccggt cgagaacagc tttccgttat cggtcggcaa
     5281 ataacgttcg tccgcggcga tcaccagaat tttatgttta ccgcgatacg gttttggata
     5341 gtccacgcca tcaagatcag agacaggact ggtatattgg ctaagcgaat attctgaagg
     5401 gaagaatgca ttatcttcag caatatcgac ctgcggattt ttacttgttt gaacagtcat
     5461 agtgtattcc ttattgttgc ttagttaggg tagtcactat atttgcgact gagctggtcg
     5521 ctatttttaa ccaggctgga aaattgagtc aatatatcga aagcggtagt cggcgggtgt
     5581 aagtatgtaa tgatgatgct gcactggtgt tgaatattat tcacgccaaa ttttccttat
     5641 tgagcaacgt cgtcgcaagt ggcctcaata attttagacc attatcaaag gtacggggcg
     5701 gcggggagag atttagtgct gtggaatatt gtggtgctga tgaatattta tatcccggat
     5761 tggtagagaa aaagcttttt ccatgcacta tttttgcaaa tatttctata tgggaggcgt
     5821 gtttgttgcg gattaattca taaagaaatg tatttggagt gttggctgga agagtagctg
     5881 gtagaagata ttgtgccagg aggggcaggg taaggggctg tatcgatatg cattgacaat
     5941 tgcgtgtgcc ggagatggag atgaaatgcc aataaaaaag cctgagttcg atgagggacg
     6001 aactcaggct agtgttcagt tttacagtaa cacgccgcgg cgcgttgact gattagaact
     6061 ggtagactaa gcccac
//
LOCUS       DAPGOF010000239         6027 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.242, whole genome shotgun sequence.
ACCESSION   DAPGOF010000239 DAPGOF010000000
VERSION     DAPGOF010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.242"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            25..453
                     /locus_tag="RHV13_004379"
     CDS             25..453
                     /locus_tag="RHV13_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243831.1"
                     /translation="MPNNTEVISCDLFSILSSLNFSVEDITGLTPAKFRKGAFLHAKI
                     SASQATRAADISLQLLTDRVSGEMNYVIVIGRHEGLLQSEAIDPNADKQYRENRHSRH
                     FIPVTDANTKALKKTCKVMKTYIKEQAFKPNTWGHNFIKG"
     gene            518..775
                     /locus_tag="RHV13_004380"
     CDS             518..775
                     /locus_tag="RHV13_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243832.1"
                     /translation="MSEKHFIVKIQNRNGDHEKSYVRILVSDCEKNACQTALISECAG
                     EVEQLSFEDGGVYDYNGENHYSVRSCVEVAPEDVATLQRYL"
     gene            931..1539
                     /locus_tag="RHV13_004381"
     CDS             931..1539
                     /locus_tag="RHV13_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243833.1"
                     /translation="MKSCFSDLPVKDGKSGTWKLDTFEITTDKALTLALRAECTGNTD
                     EFIPPGRYRRLSNGWDVVMSNTPMEIRTCQDFIERATGRVLINGLGLGMVLHAILQKD
                     DVTHVTVIEKEQDVINLVAASFATDLRVEIINADAMEYCPPAGVTYNACWHDIWTDFA
                     TANLAQMDKLESKYRDICDWQGSWGREECEQKLIEFQNLEAD"
     gene            1708..2958
                     /locus_tag="RHV13_004382"
     CDS             1708..2958
                     /locus_tag="RHV13_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDV1243834.1"
                     /translation="MSTINTDLIAHIYAASESPLTNDELYREVQRKTGMSDAELHELK
                     EFGSDKTRTSGVKHKVRWFQQTLRQAGVIERVPEKRGVWRYASKTKTNLHESWEKLCV
                     VGFSTSLGASVFGNAYAFFSNITEQIHLCLTSPPYLLRNSRDYGHGGGRGEQAYIDWL
                     LRILEPIVKQLVPGASVALNITQDSFNRGRPSRSLYLERLTLALCDKLGLELMDRLQW
                     VNRSKPPSPTHWACKQRVQLCSSYEPVLWFTNDASKVRSNNLRVLQPHSDQHLKLQAA
                     GGENRTTFYGDGAYQLKSGSFGNKTEGTIPKNTLFYGNSCADTRFCHSIARELGFPLH
                     GATSPTRLAAFLIEFLTEPGDLVVDPFAGLHKVPIAAERLGRRWLATDKIMEWLAISR
                     NLFTAAPGYKSNPMLDELAELYRT"
     gene            3239..3385
                     /locus_tag="RHV13_004383"
     CDS             3239..3385
                     /locus_tag="RHV13_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001426334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243835.1"
                     /translation="MKSDSEMASPIELHIGDHVQRHGALSEVMHIVESKCDIPGGIRV
                     AACI"
     gene            3801..4127
                     /locus_tag="RHV13_004384"
     CDS             3801..4127
                     /locus_tag="RHV13_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243836.1"
                     /translation="MHSQDPITKLTQTLQRDDGSQVRIVAQRGYGSGLTASLDVYVLR
                     RDSSESNWSLCGKDPHPEWRKMSVDEYQKFGRSEMLRYATPGEILRVASAIGQPMSFL
                     DGNPAF"
     gene            4193..4450
                     /locus_tag="RHV13_004385"
     CDS             4193..4450
                     /locus_tag="RHV13_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243837.1"
                     /translation="MNLNERSTSAATPDLSRLYLISGRIMFDDDDQAYLVEADSPGDA
                     EEAFRHHIADSADDLEKVIIVSSTSFASAHSSRVICRPAVK"
     gene            4677..5468
                     /locus_tag="RHV13_004386"
     CDS             4677..5468
                     /locus_tag="RHV13_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243838.1"
                     /translation="MADNYTLASFIIPCTQEQAKMAQEAITFVTEAEIAEGERLLDKP
                     LADCSLTEKLILSIIENHPEYDPSEPSFGQPSCPDCNYELSFATEVNSSGLSVFHGET
                     IDLDHAICLTTAVLSVFDLPEMVTITAAFTCSKSRTDEFGGMTILVTKDTHYYQDGCQ
                     FSRLMNEAHKAGIQYALCKVTHYHGESSYVASYVLSCDVADSAQEVVNRRLKACAGKE
                     PEDGIYILSEEDNTSLSVELVTELSPLDYDKLSKLLPSLDTLCGA"
     gene            5520..5969
                     /locus_tag="RHV13_004387"
     CDS             5520..5969
                     /locus_tag="RHV13_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243839.1"
                     /translation="MTNKFIVSTVVCINDFASDVPQSVSLRIDTMLEQRIRKLATYVK
                     KNDLQLTEFYFYDANWSFCGEDEIQEITDQDEYKHSDSTRQEAMLREVMPSARTECPV
                     IRVMKDSFQLSALPRHCGDDMTLNTPSIPLSELKTNVTAFITPPTYI"
BASE COUNT         1622 a         1344 c         1451 g         1610 t
ORIGIN      
        1 tgaatatgga cggggacggg taaaatgcct aataatactg aagtcattag ctgcgactta
       61 ttttcaattc tttcctcact taattttagt gttgaggata ttacagggct aactccggcc
      121 aaattcagaa agggggcttt cttgcatgca aaaatatctg caagccaggc cacgcgagca
      181 gctgatattt cccttcaatt actcacggat cgtgtttcag gtgaaatgaa ctacgtaata
      241 gtaattggca ggcacgaagg gctcctgcaa agcgaagcaa ttgacccaaa cgctgataag
      301 cagtaccggg aaaaccgtca ttctcgccat tttatcccag taaccgatgc caataccaaa
      361 gccctgaaaa aaacctgcaa ggtaatgaaa acatatatca aagagcaggc atttaagcct
      421 aacacctggg ggcacaattt cattaagggt tgatttattc ttgccccggt gaaccggggc
      481 aacgctaaat taacctcaac ctgagctgga ggcacaaatg tctgaaaaac actttatcgt
      541 aaagatccag aaccgtaacg gcgaccatga gaaaagctat gttcgtatac ttgtcagcga
      601 ttgtgagaaa aatgcttgcc agacggcact gatttccgag tgtgctggcg aggttgaaca
      661 gctgagtttt gaagacggtg gggtttatga ctacaacggc gaaaatcact acagtgtcag
      721 gagctgcgtg gaggttgccc ctgaagatgt tgcaactttg caacgctacc tttaacacct
      781 gcggcattaa aaaaaatatc gggtgtctgt tacaggatgc cgctgtttta aacgatgcct
      841 caaagatcgc tatcgctcct gatgcctgca ttaagagcgg gtttgtggta ggttcagcgt
      901 aacaaaacta ctggaatttt ggttgcctcg atgaagagct gcttttctga tttacccgtt
      961 aaagacggca agtccggaac ttggaaactg gacactttcg aaattaccac agataaggcg
     1021 ctgaccctcg cccttcgtgc cgagtgtact ggtaatacag atgagtttat cccgcccggt
     1081 agatatcgcc gcttgtccaa cggctgggat gtggtgatgt ccaatacccc gatggagatc
     1141 cgaacctgcc aggattttat agagcgagcc accgggcggg tgctcattaa tggtcttggt
     1201 cttggtatgg tgctccacgc cattcttcag aaagacgatg taacccatgt cacggtaatc
     1261 gaaaaagaac aggatgttat taacctcgtt gcagcctcgt ttgcaaccga tcttcgcgtt
     1321 gaaattatta atgctgatgc aatggagtat tgtccaccag ctggcgttac gtacaacgct
     1381 tgctggcatg acatatggac agacttcgca acagctaatc tcgcacagat ggataagctg
     1441 gaaagtaagt accgggacat ctgtgactgg cagggatcgt ggggcaggga ggagtgtgag
     1501 caaaagctca ttgagttcca gaatcttgag gctgattaat ccaacttgcg aaggtgtctc
     1561 cgctaaagga gcccggctcg ctctgtgcaa gcccagctgt gctgcatgat ttggcaaagt
     1621 cttcaacaaa ccagatcggt ctgatttagt aatctcactt ccaaccatca ggccgatata
     1681 ataactgtat ccccattagg atggctgatg agtacgatta atacggactt aattgcgcat
     1741 atctacgctg cctccgaatc gccgttgacc aacgacgagc tttatcgcga agtgcagcgt
     1801 aaaactggca tgtcggatgc cgaattgcac gaactgaaag aatttgggtc agataaaacc
     1861 agaaccagcg gcgtcaaaca taaggttcgc tggtttcagc agactctccg tcaggccggg
     1921 gtcatcgaac gcgttccaga aaaacggggc gtctggagat acgcctctaa gacgaaaaca
     1981 aacctgcatg agtcatggga aaagctctgt gttgtcggtt tctcaacttc tcttggggca
     2041 tcggtttttg gaaacgccta tgccttcttc agcaatatca ccgagcaaat tcatctttgc
     2101 ctgacctcgc caccttacct ccttcgaaat tcaagagact acgggcacgg aggggggcgt
     2161 ggcgagcagg cttatattga ctggcttctg cgtatcctcg aaccgatcgt gaaacagctt
     2221 gtaccgggcg caagtgtggc attgaatatc actcaagata gctttaatag gggtaggcct
     2281 tcacgttctc tgtatctgga gcgtctgaca ctggcattat gtgacaagct ggggcttgag
     2341 ctcatggaca ggcttcagtg ggtgaatcgc agtaagcccc catcgccaac gcactgggca
     2401 tgtaagcaac gggtgcagct ctgctcatcg tatgagcctg tgctgtggtt taccaatgat
     2461 gcgagtaagg ttcgttcaaa caacttacgt gtactacaac cgcattcaga ccagcatcta
     2521 aaattgcagg cagcaggcgg tgaaaatcgc acgacctttt atggtgatgg ggcgtaccag
     2581 cttaaaagcg gttcctttgg aaataaaaca gaaggaacga tccccaagaa tactttgttt
     2641 tacggcaata gctgcgctga tacgagattt tgccattcca ttgcccgcga gctcggtttt
     2701 ccgctgcacg gtgcaacgtc tcctaccagg cttgcagcgt ttctgattga gtttttgaca
     2761 gagccgggag atcttgttgt agatcccttc gctggcttgc ataaagtccc catcgcagcc
     2821 gagcgacttg gcagacgttg gctggcgacg gacaaaatca tggagtggct ggcgatttcc
     2881 cgaaaccttt ttacggccgc accgggatat aagagtaacc ccatgctgga tgagctcgcc
     2941 gaactgtatc ggacttaatg caagttaacg gttttgataa agtggtttga agcgacaaac
     3001 ggtttattca tttagcagtg gttccaccag agaatggcaa tattaacggc ttagcataca
     3061 ccgctgagat aatcaggtag tcattcattt ccggttgtct gtattaacga gtgtattcat
     3121 tattcacttg tttctacgat atagacccaa caaaaacaaa ttgatggttg tgatacagat
     3181 gttgttggat taactaattt ggtaatttag acggtttcta aattaaaggt ggacagatat
     3241 gaagtcagat tcagaaatgg cttctcctat cgagttgcac attggcgatc atgtccagcg
     3301 gcatggtgct ttatctgagg tcatgcatat tgttgaatcc aaatgcgata ttcccggcgg
     3361 catccgggta gcagcatgca tttgacgggt tatcggtaac gttccgggca atattcctcg
     3421 tggttggctg gaaacaccga agcgtatggc tgagcgaggc gttaagtggg cgacgagtct
     3481 gcccggaggg ctttacttta acatgcaggg gaacgcacat gcgaaagtaa gccgggttat
     3541 tcgtaatgtg actaactgaa acttttttct gaattgcggt gtaaaagtta cttttcaccg
     3601 cgtcgttttc catcactact aattcagagt ttttctgaaa taagcagagt taaagctaac
     3661 gatttattca ggccatttta ttctaatcat tatcctacac ttcaaaaata tgcaatattt
     3721 tttcttctga cctattagcc agaattctcg attcggtatc atttacttac ggtaattctt
     3781 ttaaaaacga ggtaaatggc atgcattctc aggatcctat cacgaaatta acccagacgt
     3841 tgcaacgcga cgatggttct caggttcgta ttgtagcgca gcggggatat ggaagcgggc
     3901 ttacagcctc gcttgatgtg tacgttctcc gtcgtgattc ctctgaaagc aactggtcac
     3961 tgtgcggaaa agatcctcac cccgagtgga gaaagatgtc agtagatgag tatcagaaat
     4021 ttggacgctc agaaatgctg cgctatgcca caccaggtga aattctcagg gtggcatccg
     4081 ctattggcca gccaatgagc ttcctcgatg gcaaccctgc gttttagcct gaaccctggc
     4141 gacgcctttt aaaccggcgt cattcttttt acgagcaaca gacggagaaa aaatgaatct
     4201 gaacgaacga agcacgagcg cagcgacgcc agatttgagc cgactttatc tcatttccgg
     4261 gcgcattatg tttgatgatg atgatcaggc ctacctggtc gaagccgatt cccctggtga
     4321 tgcagaggag gcttttcggc atcatattgc cgacagtgcc gacgacttag aaaaggtgat
     4381 tatagtttcc tctacaagtt tcgcttcagc tcacagcagt cgcgtaattt gtcggcctgc
     4441 cgtgaagtaa tttcagcttc caatgatatt tccctcttta taaaagccgc cgtctggcgg
     4501 tttttatttg caaaaatgaa tattaatcct gaacaatatt aatttaagat gaggtggtgt
     4561 tcatattctt cttaatattt tttctaaaaa aacgacttca ggttattagt tggtagtagt
     4621 gtgtttgtta tgatcttctc atcaaataca ttaattaaat aatcaaggag tcaactgtgg
     4681 cagataatta tactctggcg tcgtttatta ttccctgcac tcaggagcag gctaaaatgg
     4741 cacaagaagc aatcacattc gttaccgaag cagaaattgc agaaggtgag cgtttgcttg
     4801 ataagccact ggcagattgt tctctgactg agaagctgat tctcagtatt attgagaacc
     4861 accctgagta tgacccttca gagccgagct ttgggcaacc atcctgccca gactgtaatt
     4921 atgaactgtc gttcgcaaca gaagttaaca gcagtgggct gtcagttttt catggagaga
     4981 ctattgatct tgaccatgca atttgcctca caactgccgt gctgtcggta ttcgacctcc
     5041 cggaaatggt aacaattact gctgcattta catgcagtaa aagccggaca gatgaatttg
     5101 ggggtatgac tattctggtc acaaaggata cccactatta ccaggatggc tgtcagtttt
     5161 ctcgtctcat gaatgaggct cacaaagccg gtatccagta tgctctgtgt aaagtgacgc
     5221 attaccacgg tgagagcagc tatgtggcaa gctatgtcct gagctgcgac gtagcggatt
     5281 cagcccagga ggtcgttaac agacgactga aggcatgtgc cggaaaagag ccagaagacg
     5341 gaatctatat tctgagtgaa gaagacaaca ccagtctttc ggtcgaactc gttacagaac
     5401 tgtctccgct cgattacgac aagctgagca agcttcttcc ttcccttgac accctttgcg
     5461 gcgcataagc ctgcctgata accgtagcca ccggttcccc cggtggcctg gagcctgcca
     5521 tgaccaataa attcatagta tctaccgttg tttgcatcaa tgactttgca agtgatgttc
     5581 cgcaaagtgt ttccttacgg atagatacaa tgcttgaaca gcgtatccgc aaattggcga
     5641 catacgtgaa aaaaaacgat cttcagttaa ctgagtttta cttctatgac gctaactggt
     5701 cattttgtgg tgaagatgaa attcaagaaa taacagacca ggatgaatat aagcatagcg
     5761 atagcacaag gcaggaagcg atgctgcggg aagtaatgcc atcagcacgt acggaatgcc
     5821 cggttattag ggtgatgaaa gattcatttc agctttctgc tctaccacgc cattgtggtg
     5881 atgacatgac tcttaacact ccttcaattc cgctgtctga gttgaaaaca aatgtcacgg
     5941 cttttattac accaccaacc tatatctaat agctgtgctg ggagattaaa ttacaatggg
     6001 tttttatttt aaatctattt aagaatt
//
LOCUS       DAPGOF010000240         6012 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.243, whole genome shotgun sequence.
ACCESSION   DAPGOF010000240 DAPGOF010000000
VERSION     DAPGOF010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6012
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.243"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            154..600
                     /locus_tag="RHV13_004388"
     CDS             154..600
                     /locus_tag="RHV13_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDV1243840.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATMALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQTLSS"
     gene            611..2062
                     /locus_tag="RHV13_004389"
     CDS             611..2062
                     /locus_tag="RHV13_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HDV1243841.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTCVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            2052..3122
                     /locus_tag="RHV13_004390"
     CDS             2052..3122
                     /locus_tag="RHV13_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDV1243842.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSREEAKKRGAEIGDFISPEAN
                     FACWGEDKIVGKALDNRIGCAMMAELLQTVNNPGITLYGVGSVEEEVGLRGAQTSAEH
                     TKPDVVIVLDTAVAGDVPGIDNIKYPLKLGNGPGLMLFDKRYLPNQKLVAALKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            3122..4870
                     /locus_tag="RHV13_004391"
     CDS             3122..4870
                     /locus_tag="RHV13_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HDV1243843.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTVCDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EHSGLFLGDNAVRTLTGLIEKQHQQAQIISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREDEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQSVEVNNEVINHVLIACAAADARHELKIFSYLASVLYQHPAETI
                     VGLTGYEGFMALLRQG"
     gene            complement(4972..>6012)
                     /locus_tag="RHV13_004392"
     CDS             complement(4972..>6012)
                     /locus_tag="RHV13_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HDV1243844.1"
                     /translation="LSSAIIVAIMALGLTGCDDKKAETETLPPANSQPAAPAPEAKPT
                     EAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQRSLARYADWLKDFK
                     QGPTGKESTVYGIYGISESSLAECEKGVKRAVALTPALQPIDGVAVSYIDAAVALGNT
                     INEMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKRQLAEL
                     KNIEEREGKTFHYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADK
                     GGMNFSFINSADQYQLEAKKYVRRVRDKVPYSDWDKEQLQDANSSWMVEDSFPRALRE
                     YNEMVDDYNRLR"
BASE COUNT         1396 a         1535 c         1522 g         1559 t
ORIGIN      
        1 agatttattt tacctactaa tatgtgcctt acgccgcaaa atttatcctt cactctgctt
       61 tatattgtca cgacttcacg acatatccgc gctcatcaca aacccgcccc gctattacaa
      121 ttaactgaaa tattttttca tggagtccga ttaatggcag ctcttactgc aagctgtatt
      181 gacctgaata ttcagggcaa tggcgcttat tccgttctga agcagttggc gacaatggcg
      241 ttacaaaacg gttttatcac cgactcacac cagttcctgc aaaccctgct gctgcgcgaa
      301 aaaatgcact ccaccggttt tggttctggt gtcgccgtgc cgcacggtaa aagcgcctgc
      361 gttaaacaac cgttcgtgtt attcgcccgc aaagcgcagg ctattgactg gaaagccagc
      421 gatggcgaag acgtcaattg ctggatctgc ctcggcgtgc cgcaaagcgg cgaagaggat
      481 caggtcaaaa tcatcggcac actgtgtcgc aaaattatcc accaggattt tattcatcaa
      541 ctgcaacagg gcgacgccga ccaggtgctt gccttgttaa atcaaaccct cagctcataa
      601 ggaagtggcg atggagtcat ccttacgtat tgtcgcgatc accaactgcc ccgccgggat
      661 cgctcacacc tacatggtgg cggaagccct ggaacagaaa gcccgttctc tcggtcatac
      721 cataaaagtg gaaactcaag ggtccagtgg cgttgaaaac cgcttatcca gcgaagagat
      781 tgccgctgcc gattacgtca ttctcgctac cgggcgtggc ctgagcggtg atgatcgcgc
      841 gcggtttgcc gggaagaaag tttatgagat tgccatctcc caggcgttga aaaatatcga
      901 ccagattttc agcgaattac cgacaaactc gcagcttttt gccgcagata gcggcgtgaa
      961 gcttggcaaa caggaagtgc aaagcggcag cgtgatgagc cacctgatgg cgggcgtctc
     1021 tgcggcgctg ccgtttgtca tcggcggtgg tatcctggtg gcgcttgcca acatgctggt
     1081 gcagttcggt ttgccgtata ccgatatgtc gaaaggcgcg ccgtcgttta cctgcgtagt
     1141 tgaatccatc ggttaccttg gcttcacctt tatgatcccc atcatgggcg cttatattgc
     1201 ctcgtcgatt gccgataaac cggctttcgc tccggcgttt ctggtttgtt atctggcgaa
     1261 cgacaaagcg ctgctcggca cccagtcggg tgcgggattc ctcggcgcgg tggtgctggg
     1321 gctggcgatt ggctatttcg tcttctggtt ccgtaaagtc cgtcttggca aagcattaca
     1381 accgctgctc ggttcaatgc tgatcccgtt cgttactctg ctggttttcg gcgtgctcac
     1441 ttactacgtt atcggaccgg tgatgtccga cctcatgggc gggctactgc acttcctcaa
     1501 taccattcct ccatcaatga agtttgcggc agcgtttctg gtcggtgcga tgctggcgtt
     1561 cgatatgggc ggccccatca ataaaaccgc ctggttcttc tgcttctcac tgctggaaaa
     1621 acacatttat gactggtacg ccatcgtcgg cgttgtcgcg ctgatgccgc ccgtcgcggc
     1681 gggtcttgcg acgttcattg cgcccaaact gtttacccga caggaaaaag aggctgccag
     1741 tagtgccatt gtggtcggtg ccacagtcgc aaccgaaccg gctattcctt acgctctggc
     1801 agctccgttg ccgatgatta ccgccaatac gctggcgggc ggtattaccg gcgtgctggt
     1861 gatcgctttc ggaatcaaac gtctggcacc tggtttgggt atctttgacc cgctgatcgg
     1921 cctgatgtcg ccggtaggtt cgttttatct ggtgctggcg attggcctcg cgctgaacat
     1981 ttcattcatt atcgtcctga aaggattgtg gttacgccgc aaagcgaaag ccgcgcaaca
     2041 ggagcttgtc catgaacatt gagttactgc aacagttgtg cgaagccagc gccgtcagcg
     2101 gcgatgaaca ggaagttcgc gacattctga taaacacgct ggaaccttgc gttaatgaga
     2161 tcacctttga tggtctgggc agctttgttg cccgtaaggg aaataaaggt ccaaaagttg
     2221 ccgttgtcgg gcatatggat gaagtcggct ttatggtcac ccacatcgac gagagcggtt
     2281 ttctgcgctt taccaccatt ggcggctggt ggaatcagtc gatgctcaac caccgggtaa
     2341 caatacgcac acacaaggga gtgaaaatcc ctggtgtgat tggttccgta gcgcctcatg
     2401 cgttaacgga aaagcaaaag caacaaccgc tgtcatttga tgagatgttc attgatattg
     2461 gcgcgaacag tcgcgaagaa gcgaaaaaac gcggtgcgga aattggcgat tttattagcc
     2521 cggaagccaa ttttgcctgc tggggcgaag ataaaatagt cggcaaggcg ctggataatc
     2581 gcatcggctg cgcgatgatg gctgagctac tacagacagt aaataaccca ggaattacgc
     2641 tgtacggcgt cggcagcgtg gaagaagaag ttgggctacg cggggcacaa acctcggctg
     2701 aacacactaa accggatgtg gtgatcgtgc tggataccgc cgtcgcgggc gatgttccgg
     2761 gcattgataa cattaaatac ccgctgaaac tgggcaacgg gccggggctg atgctgtttg
     2821 acaagcgcta cttacccaac cagaaactgg tggcggcgtt aaaaaactgt gccgcacata
     2881 acgatttacc gctgcaattt tccaccatga aaaccggtgc gacggatggc gggcgctaca
     2941 acgtgatggg cggcgggcgt ccggttgtcg cgctgtgtct gccaactcgt tatctgcacg
     3001 ccaacagcgg catgatttca aaagccgatt acgatgctct gctcacgctg atacggggtt
     3061 ttctgacgac cttaaccgcg gagaaagtca acgcgtttag tcagttccgt caggtggatt
     3121 aatgctaaac gaacgccagt taaagattgt cgatctgctg gagcaacagc cgcgcacgcc
     3181 tggcgagctg gcgcaacaga ctggcgtttc aggcaggacc atcctgcgtg atattgacta
     3241 tctcaacttt acccttaacg gcaaagcccg tatctctgcc agtggcagtg cgggctatca
     3301 gttggaaatc ttcgagcgcc gcagcttttt tcagttgctg caaaagcacg ataacgacga
     3361 tcggctgcta gcgctgttat tactgaatac ctttactccc cgtgcgcaac tcgcctcggc
     3421 gcttaatttg ccagaaacgt gggtagcaga gcgtctgccc cggttaaaac agcgttatga
     3481 acgcacttgt tgcctggcca gtcgccccgg tttgggccat ttcattgatg aaacagaaga
     3541 gaaacgcgtt atcttgctgg cgaacttgct acgcaaagat ccgtttttaa tacctttggc
     3601 tggcgtaaca cgagataacc ttcagcattt atccacggtc tgcgacaacc aacaccgctg
     3661 gccgctcatg cagggtgatt atctctccag cctgattctg gcgatttacg ccctgcgtaa
     3721 tcaactgacc gatgagtggc cgcaatatcc cggtgacgag ataaaacaaa tcgttgaaca
     3781 tagcggtctg tttcttggtg ataacgctgt aagaaccctg acgggtttga tagagaaaca
     3841 acatcagcaa gcgcaaataa tttctgccga tcatgtgctg gggttgctgc aaagggtgcc
     3901 aggcatcgcg tcattgaata ttattgatac gcagttggtt gagaacatta ccggtcattt
     3961 attacgttgc cttgccgcac cggtgtggat tgccgagcac cgccaaagca gcatgaataa
     4021 cctgaaagcc gcctggcctg cggcctttga tatgagtctg cactttatta cgctactgcg
     4081 tgaacagctc gatattcccc ttttcgacag cgatctgatc ggtttgtatt ttgcctgtgc
     4141 gctggaacga catcaaaacg aacgccagcc gatcattttg ctctcggacc agaacgcgat
     4201 tgccactatt aatcagctcg ccattgagcg cgatgtttta cattgtcggg tgattattgc
     4261 ccgtagctta agcgaacttg ttgccattcg cgaagagatt gagccgttat tgatcattaa
     4321 caacagccat tatttactgg atgatgcggt aaataattac atcaccgtaa aaaatatcat
     4381 tacggctgcc ggtatcgaac aaataaaaca ttttctggcg acggcattta ttcgccagca
     4441 accggagcgt tttttctctg cccccggaag ttttcattat tcgaatgtac gcggtgaaag
     4501 ctggcaacat attacccggc aaatttgtgc gcaattagtc gcacaacacc atattaccgc
     4561 cgatgaagcg caacggatca tcgcccgcga agacgaaggt gaaaacctga ttgttaatcg
     4621 cctcgccatc ccacattgct ggagcgaaca ggagcgacgt tttcgtggat tttttattac
     4681 cctcgcccaa tcagttgagg tgaataacga agtcattaac catgttttga tcgcctgcgc
     4741 cgccgccgat gcgcgtcatg agctaaaaat attcagctat cttgccagcg tcctgtatca
     4801 gcatccggcg gaaactattg tcggactaac ggggtatgaa gggtttatgg cgttgcttcg
     4861 ccaggggtga atgatgtcgg atgcaccgct aacacggctt aacgacgttt aggtcggata
     4921 aggcgtttac gccgcatccg accttgtatt tctcagcaaa aacaaaaacg tttaacgcag
     4981 gcgattataa tcatcgacca tttcgttgta ctcgcgtaat gctctcggga aagagtcttc
     5041 gaccatccag cttgagtttg cgtcctgaag ttgctcttta tcccagtcag agtacgggac
     5101 tttatctctg acgcggcgaa catatttttt agcctcgagt tgatactggt ctgccgaatt
     5161 aatgaacgag aaattcatac cgcctttatc cgcttctttg gcctgcgcca ccagcgtttc
     5221 cagttcagag actttcttca ttgccgcatc aacatcaaac ttctcttgcg aaatcaggtt
     5281 attaatttgt ttcgctgaaa tcatgactgc cagagagtag tagtggaatg ttttcccttc
     5341 tctttcttca atatttttca gttccgcaag ctggcgctta tcattaattt cctgaatcgc
     5401 cgcatgataa gattctgcta caggttcaaa ggcttccaga ttttttaaga atgtctggtg
     5461 cagcgttttc cctttcgcaa acgcatcgtc tttataattt tcctgggtgt aatatttatc
     5521 catttcgtta atggtattac ccaacgccac ggcagcatca atataactca ccgccacgcc
     5581 atcaataggt tgcagcgcag gcgttaacgc cacagctctt tttacgcctt tttcacactc
     5641 agcgaggctg gattcactaa tgccgtagat gccataaaca gtactctctt taccggtagg
     5701 cccctgttta aaatctttca gccagtcagc ataacgcgcc agactgcgct gtaccgggat
     5761 ctgtaacttg ttgtagcact tgatatagac atccattttt tcgtcgaaaa ccgcttgttc
     5821 atcgaccacc ggttgcgcag gtgtttcagg tttagcttct gcttttgcaa cgggagcttc
     5881 agtaggtttc gcttcaggag ctggtgcggc aggttgacta ttggcaggcg ggagcgtttc
     5941 tgtttcggct tttttatcat cacaacccgt cagaccgagg gccatgatgg cgactataat
     6001 agcggaagat aa
//
LOCUS       DAPGOF010000241         6003 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.244, whole genome shotgun sequence.
ACCESSION   DAPGOF010000241 DAPGOF010000000
VERSION     DAPGOF010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6003
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.244"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            70..1125
                     /gene="phoE"
                     /locus_tag="RHV13_004393"
     CDS             70..1125
                     /gene="phoE"
                     /locus_tag="RHV13_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HDV1243845.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPISGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(1164..1565)
                     /gene="crl"
                     /locus_tag="RHV13_004394"
     CDS             complement(1164..1565)
                     /gene="crl"
                     /locus_tag="RHV13_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HDV1243846.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(1623..2867)
                     /gene="frsA"
                     /locus_tag="RHV13_004395"
     CDS             complement(1623..2867)
                     /gene="frsA"
                     /locus_tag="RHV13_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HDV1243847.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(2959..3417)
                     /gene="gpt"
                     /locus_tag="RHV13_004396"
     CDS             complement(2959..3417)
                     /gene="gpt"
                     /locus_tag="RHV13_004396"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDV1243848.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3678..5135
                     /gene="pepD"
                     /locus_tag="RHV13_004397"
     CDS             3678..5135
                     /gene="pepD"
                     /locus_tag="RHV13_004397"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HDV1243849.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVIHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREEVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQDILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(5192..5713)
                     /locus_tag="RHV13_004398"
                     /pseudo
     CDS             complement(5192..5713)
                     /locus_tag="RHV13_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(5712..5915)
                     /locus_tag="RHV13_004399"
                     /pseudo
     CDS             complement(5712..5915)
                     /locus_tag="RHV13_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="mutations in this gene result in a compromised
                     ability for drug-inducible mexXY expression; expression is
                     inducible by the same ribosome-targeting agents that
                     induce mexXY; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
BASE COUNT         1542 a         1521 c         1474 g         1466 t
ORIGIN      
        1 aaaactttcc tgttttcaac ggggctctcc cgctgaatat tcgcgcgtta attaaaatca
       61 ggaatgaaaa tgaaaaagag cactctggca ttagtggtga tgggcattgt ggcatctgca
      121 tccgtacagg ccgcagaaat atataacaaa gacggtaata aactggatgt ctatggcaaa
      181 gttaaagcca tgcattatat gagtgataac gacagtaaag atggcgacca gagttatatc
      241 cgttttggtt ttaaaggcga aacacaaatt aacgatcaac tgactggtta tggtcgttgg
      301 gaagcagagt ttgccggtaa taaagcagag agtgatactg cacagcaaaa aacgcgtctc
      361 gcttttgccg ggttgaaata taaagatttg ggttctttcg attatggtcg taacctgggc
      421 gcgttgtatg acgtggaagc ctggaccgat atgttcccgg aatttggtgg cgactcctcg
      481 gcgcagaccg acaactttat gaccaaacgc gccagcggtc tggcgacgta tcggaacacc
      541 gacttcttcg gcgttatcga tggcctgaac ttaaccctgc aatatcaagg gaaaaacgaa
      601 aaccgcgacg ttaaaaagca aaacggcgat ggcttcggca cgtcattgac atatgacttt
      661 ggcggcagcg atttcgccat tagtggggcc tataccaact cagatcgcac caacgagcag
      721 aacctgcaaa gccgtggcac aggcaagcgt gcagaagcat gggcaacagg tctgaaatac
      781 gatgccaata atatttatct ggcaactttt tattctgaaa cacgcaaaat gacgccaata
      841 tctggcggct ttgccaataa aactcagaac tttgaagcgg tcgctcaata ccagtttgac
      901 tttggtctgc gtccatcgct gggttatgtc ttatcgaaag ggaaagatat tgaaggtatc
      961 ggtgatgaag atctggtcaa ttatatcgat gtcggggcta catattattt caacaaaaat
     1021 atgtcagcgt ttgttgatta taaaatcaac caactggata gcgataacaa attgaatatt
     1081 aataatgatg atattgtcgc ggttggcatg acctatcagt tttaatgatt attgccggat
     1141 gtgatgcatc cggcacattt cactcacgcc gttaacttca ccggctcgtc acgaaaatca
     1201 tccgccggtt ccagcttcag attcagcgtc gtcagcagtt cacgcagctt ctcgtgaaac
     1261 tcacgcaggg tgtgctccag tcgttcaacc acttcagtat cttttaccgg aacactcttc
     1321 cagtcgcctg ctttatcgaa cagaccaaac tggtagctgt aggtaaaacg ggattcctgc
     1381 gcttccagct ccatccacca gccccagaat tcacgcactt ccggtgccgg tttcacgttg
     1441 acgcatacag ccagacaatc gaaaaagaat cgattatctt cgcacttacc ttcacgaata
     1501 tacgggccta gtgcggtaaa ttttttgatc aatctgctct tcgggtgtcc actcggtaac
     1561 gtcattgcta tctcctgttg tgatgcaact gttttaccaa attggcaaaa tttagcaaat
     1621 ttttaacaca agcgtttttc gatccagccg gtgatttcct gaagaccttt gtcaaaattc
     1681 cgatacaccg ggttaaatgg gatctctaat aatttaccgt cagcagatga tgaggtgatt
     1741 aagcgtgagt cctcttccgg gctgaacgga tcgttcttcc agtagcctga taacattggc
     1801 gttgggcagc gacgtcccag caatccttgc acttttaatg aatagcgatt cagctccacg
     1861 cgcaacgcgt catcggaagc atcatgcatc cccaaacgac tcgccagaac gtcaagatac
     1921 atttccggca cctgttgctg gcacttaaaa tcactcaaca gggtatgaac taccggacca
     1981 agacaggcaa ccgctttcag acgcggcgat tcaaggtatg ccagacgcac ggcaacgtta
     2041 gcgccgaaac ggaaaccaaa ggccgcgacg cgagtgtgat ccacccacgg tacgttaggc
     2101 agcgccttta agacgtgctg atgcaacagg ctggagtcct gggtgagctt ccattttgaa
     2161 gaaaagccca ccgacggcat atcaatagtc agcatcgcaa tgccgcgcgg cgcaaaataa
     2221 cgttcataca ggctgtaata gtccgtctgc atcgcatcca gaccaccaca cattaatact
     2281 gtcgggaacg ggccatcgcc tttcggcata tgcaaaaagc cggtgatggg cgcaccgccg
     2341 ggtacggtaa actccatctg ccgcatcgtg cccggcagac gctgggcggc ctcttcatag
     2401 gcgcggtttg ataaagcctg cgcttgctcg gccaggtcat ctcctttcag atgaggatag
     2461 gcggcaatgt tgtacaacgt agccgcatgc agccagtgtc gaccactgag ttgcggatct
     2521 tcttccgcac aggctttttg ttgccacacc atcgcctggg tggcccactc ataaatccag
     2581 ttgccaccac ggtagccaat cacggtatcg tataaatcat catcggtacg ttcagcatcg
     2641 ctcatcacaa tacgtgcctg gacgtcgagg atttcacgtg ggtcaatgcc gcgccagatc
     2701 cacatcagac ggttaatcat gcgataccag tgagggatgg ttttaccatc aagggccgac
     2761 tgcacaggcg gttgtgcgcc gtgattaaag cggcggacta gcgtcgaggt ttcaggatgt
     2821 ttaaagcggg gtttgaacag ggtttcgctc aggtttgcct gtgtcatgga tgcagcctcc
     2881 agaatactta ctggaaacta ttgtaacccg cctgaagtta aaaagaacaa cgcccggcag
     2941 tgccaggcgt tgaaaagatt agcgaccgga gattggcggg acgaatacga cgcccatatc
     3001 ccacggctgt tcgatccagg tatcttgcgg gatatcaaca acataatcat caaccagcgg
     3061 acgaccagcc ggttttgcaa agatggtgac aaagtgcgct tttggataca tttcacgaat
     3121 cgcaaccgca gtaccaccgg tatccaccag gtcatcaata acgatgaagc cttcgccatc
     3181 gccttctgcg cgtttcagca ctttaagctc gcgctggttg tcgtgatcgt agctggaaat
     3241 acaaacggta tcgacatgac gaatacccag ttcacgcgcc agtaacgcac ccggtaccag
     3301 accgccacgg cttacggcaa taatgccttt ccattgttca gaaggcatca gtcggcttgc
     3361 gagtttacgt gcatggatct gcaacatgtc ccaggtgacg atgtattttt cgctcatgtg
     3421 aagtgtccca gcctgtttat ctacggctta aaaagtgttc gaggggaaaa taggttgcgc
     3481 gagattatag agatctggcg cactaaaaac cagtatttca catgagtccg cgtcttttta
     3541 cgcactgcct ctccctgacg cgggataaag tggtattctc aaacatatct cgcaagcatg
     3601 tcttgtgttg acaacatttt ctgctaaccc tgtgacctgc aatactgttt tgcgggtgat
     3661 cgacaaggag acttaacgtg tctgaactgt ctcaattatc tccacagccg ctgtgggata
     3721 tttttgccaa aatctgttct attcctcacc cgtcctatca tgaagagcaa ctcgctgaat
     3781 acattgttgg ttgggcaaaa gagaaaggtt tccatgtcga acgcgatcag gtaggtaata
     3841 tcctgattcg taaacctgct accgcaggta tggaaaatcg taaaccggtc gtcttgcagg
     3901 cccacctcga tatggtgccg cagaaaaata acgacactgt gcatgacttc acgaaagatc
     3961 ctatccagcc ttatattgat ggcgaatggg ttaaagcgcg cggcaccacg ctgggtgcgg
     4021 ataacggcat tggtatggcc tctgcgctgg cggttctggc tgacgaaaac gtgattcacg
     4081 gcccgctgga agtgctgctg accatgaccg aagaagccgg tatggacggt gcgttcggct
     4141 tacagagcaa ctggttgcag gctgatattc tgattaacac cgactccgaa gaagaaggtg
     4201 aaatctacat gggttgcgcg gggggtatcg acttcacctc caacctgcat ttagatcgtg
     4261 aagaggttcc agctggtttt gaaaccttca agttaacctt aaaaggtctg aaaggcggtc
     4321 actccggcgg tgaaatccac gttggcctcg gtaatgccaa caaactgctg gtgcgcttct
     4381 tggcgggtca tgcggaagaa ctggatctgc gccttatcga tttcaacggc ggtacactgc
     4441 gtaacgccat cccgcgtgaa gcctttgcga ccattgctgt cgcagctgat aaagtcgacg
     4501 cactgaaatc tctggtgaat acctatcagg acattctgaa aaacgagctg gcagaaaaag
     4561 agaaaaatct ggccttgttg ctggactctg tagcgaacga taaagctgcc ctgattgcga
     4621 aatctcgcga tacctttatt cgtctgctga acgccactcc gaacggtgtg atccgcaatt
     4681 ccgacgtggc aaaaggtgtg gttgaaacct ccctgaacgt cggtgtggtg accatgactg
     4741 acaataacgt agaaattcac tgcctgatcc gttcactgat cgacagcggt aaagactacg
     4801 tggtgagcat gctggattcg ctgggtaaac tagctggcgc gaaaaccgaa gcgaaaggcg
     4861 catatcctgg ctggcagccg gacgctaatt ctccggtgat gcatctggta cgtgaaacct
     4921 atcagcgtct gttcaacaaa acaccgaaca tccagattat ccacgcgggc ctggaatgtg
     4981 gtctgttcaa aaaaccgtat ccggaaatgg acatggtttc tatcgggcca actatcaccg
     5041 gtccacactc tccggatgag caagttcaca tcgaaagcgt aggtcattac tggacgctgc
     5101 tgactgaact gctgaaagaa attccggcga agtaattatt tgatttgctg ccggatggcg
     5161 tttaatcgcc ttccggcagt ttcatccttc attatccttc gataaaagcc atccctgtaa
     5221 atgtccgtct cgggttgcca cgttcaatct ggtgatggaa cattcgccgc tgcgatttca
     5281 gcaccgcgct attttcctgt tgctgctgct ccagcctcca ggcaatcagc aatcgtgcca
     5341 gccgcttgtt agcatgctga ctacgctctg actgaacctt cacgctaata ccggatgcca
     5401 aatgcgtggc gcgtaccgcc gagtcggttt tattgacatg ttgaccgccc ggccccgacg
     5461 aacgcaacgt ctcataacgg attgcatccg attgttcctg ctcatcagcg gtaaaacgcc
     5521 caatgcccag aaaccagttt ttgcgcccat gatgaggccg atacggactc ggacaaatcc
     5581 actgaatagt gccacaccag ctttcgctta atgcccatgc gttatcgcca tccagagaaa
     5641 ccagcgccga acgcagcgtg tcagagtagc agcccgtttc tgtttccagc accgttaccg
     5701 cgacgtcttg tcgggtaaaa acgaccaaca gagaagaacg ctgcgccaat cgggtagccg
     5761 cgtccggggt gtaaatctgg catccccact acgcgctgca tgttgggtaa atttgccgtg
     5821 gtgtgtaatt gttggatcgc taaactctcg atccacaggt catcagatgc gatggtaaat
     5881 accgcatcgg ataagggaag aatatatttg cccatgtacc attccattta tactcgtcat
     5941 acttcaagtt gcatttactg cgactgcgtt cgttcacccc agtcacttac ttatgtaagc
     6001 tcc
//
LOCUS       DAPGOF010000242         5927 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.245, whole genome shotgun sequence.
ACCESSION   DAPGOF010000242 DAPGOF010000000
VERSION     DAPGOF010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5927
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.245"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(46..237)
                     /gene="yaiA"
                     /locus_tag="RHV13_004400"
     CDS             complement(46..237)
                     /gene="yaiA"
                     /locus_tag="RHV13_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HDV1243850.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(287..811)
                     /gene="aroL"
                     /locus_tag="RHV13_004401"
     CDS             complement(287..811)
                     /gene="aroL"
                     /locus_tag="RHV13_004401"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HDV1243851.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(994..1452)
                     /locus_tag="RHV13_004402"
     CDS             complement(994..1452)
                     /locus_tag="RHV13_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HDV1243852.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            1572..2381
                     /gene="proC"
                     /locus_tag="RHV13_004403"
     CDS             1572..2381
                     /gene="proC"
                     /locus_tag="RHV13_004403"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDV1243853.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(2398..3513)
                     /gene="adrA"
                     /locus_tag="RHV13_004404"
                     /gene_synonym="dgcC"
     CDS             complement(2398..3513)
                     /gene="adrA"
                     /locus_tag="RHV13_004404"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HDV1243854.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYRDWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(3615..3935)
                     /gene="psiF"
                     /locus_tag="RHV13_004405"
     CDS             complement(3615..3935)
                     /gene="psiF"
                     /locus_tag="RHV13_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HDV1243855.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(4054..5469)
                     /gene="phoA"
                     /locus_tag="RHV13_004406"
     CDS             complement(4054..5469)
                     /gene="phoA"
                     /locus_tag="RHV13_004406"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HDV1243856.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTADQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(5570..5830)
                     /gene="iraP"
                     /locus_tag="RHV13_004407"
     CDS             complement(5570..5830)
                     /gene="iraP"
                     /locus_tag="RHV13_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HDV1243857.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT         1391 a         1584 c         1445 g         1507 t
ORIGIN      
        1 cccttatagt ggcactcaga atcccttccg agcagtctga tgcggtcact ctttgtcagg
       61 gtcctcatag cgttttttcg catccatcgc ttcctgagca gtaggatgtt cactgatcaa
      121 cgagtctggt ttaggatgat cggctctgag ttggtaccag gttaccgtct gtcctggctt
      181 tcctttttca atcgtcacta tatatgcttc acgaggataa ggcggtttcg ttggcatcgt
      241 tcttccttat ttcacgggat gaacgttaag tataggcgct cgaaaatcaa caattgatcg
      301 tctgtgccag ggcgctgcga atttcagaaa tcacctggct gggttcgttt gttgcgtcga
      361 tgataatatg cgcaacttcg cgatatagcg catcgcgttc ttccagcact tcctgaactt
      421 cttcgctcag cggttttccc gttaaggttg gccgtaaatc ttcttccggt gcagcttgca
      481 gtcggttaac caggactgat actggcgcac acaaataaac cacgatcccg ttattttgca
      541 tgaagtgacg attaaattcc gtcagaataa tgccgccgcc tgtagcgata acggtggatg
      601 gcgcagttac cgcttccagc gccgccgttt ctctggcgcg aaatcccgcc cactcttccc
      661 tttcgacgat ctccgcgacc gtcatattga gctgtgattg caaccactga tcggtatcga
      721 caaaccgacg gttaagcgaa tcggcaaggg ccattccgac cgttgtttta ccacagcccc
      781 gaggcccaat cagaaaaaga ggttgtgtca tcgtgggttt tccccaatag gtcgcaatgc
      841 ggcgaaagcc ggtgtcatga gaatagcgat cataccatca aactagtaca atttcgattg
      901 taaagaaaaa attccactta aagtgaaaat ctcaatacat cccttactat accaataaat
      961 attcaagaat gaagtgtaaa taataaatta catttagcca cgactacgtt gcacttccag
     1021 ccaccacttc tccagctccg cggcaaatgc ctggcggtca cgttgtgaaa ggctatctgg
     1081 tccgccggtc tggatcccac tggcacgtaa ggtatccata aaatcgcgca tcgtcaggcg
     1141 ctcacgaatg gtcgctggcg tgtaacgttc accgcgcgga ttaagcgccg cagcgccttt
     1201 ctcgatggct tcagcagcca aaggtatatc tgcggtgatc accaaatcgc ccgcttcaca
     1261 ctgccggaca atttcgttat cggcaacgtc gaaacctgcc gcgacgcgca gcgtacgaat
     1321 aaatcgcgat ggcggcacgc gtaaactctg gtttgctacc agtaccagcg gcatctgcat
     1381 acgttccgcc gcgcgataca aaatctcttt aattacattg ggacacgcgt cggcatccac
     1441 ccaaattgtc ataaagtcat cctttgttgg gtaatcctct attgtgtcgc gcttttgcct
     1501 tccggcatag ttctgtttat gcttctgcca gcgattatca aaacaatgaa tttcacggca
     1561 ggagtgaggc aatggaaaag aaaatcggtt ttattggctg cggcaatatg ggaaaagcca
     1621 ttctcggcgg tctgattgcc agcggtcagg tgcttccagg gcaaatctgg gtatacaccc
     1681 cctccccgga taaagtcgcc gccctgcatg accagttcgg catcaacgcc gcagaatcgg
     1741 cgcaagaagt ggcgcaaatc gccgacatca tttttgctgc cgttaaacct ggcatcatga
     1801 ttaaagtgct tagcgaaatc acctccagcc tgaacaaaga ctctctggtc gtttctattg
     1861 ctgcaggtgt cacgctcgac cagcttgccc gcgcgctggg ccatgaccga aaaattatcc
     1921 gcgccatgcc gaacactccc gcgctggtta atgccgggat gacctcggta acgccaaacg
     1981 cgctggtaac cccagaagat accgctgatg tgctgaatat tttccgctgc tttggcgaag
     2041 cggaagtaat tgctgagccg atgatccacc cggtggtcgg tgtgagcggt tcttcgccag
     2101 cctacgtctt tatgtttatc gaagcgatgg ccgacgccgc cgtgctgggc gggatgccac
     2161 gtgcccaggc gtataaattt gccgctcagg cggtaatggg ttccgcaaaa atggtgctgg
     2221 aaacgggaga acatccgggg gcactgaaag atatggtctg ctcaccggga ggcaccacca
     2281 ttgaagcggt acgcgtactg gaagagaaag gcttccgtgc tgcagtgatc gaagcgatga
     2341 cgaagtgtat ggaaaaatca gaaaaactca gcaaatcctg atgactttcg ccggacgtca
     2401 ggccgccact tcggtgcggt tacgtccggc tttctttgct ttgtaaagcg ccaaatctgc
     2461 cgatttcaac caatcacgat agtgactcat ttgtgggttc agcggcgcaa cccccacact
     2521 aatccgtaaa gttacctgtg gcgtattcgg caaacgtaat gtatttagcc cttcatgcac
     2581 ccgtaacatg gcggtaatgg cgctctcagc tggcgtaccg gacatgatta ctgcaaactc
     2641 atcgccgcca aaccgaccaa tcacatcgct accgcgcagg gtaatttgta actgtcgggt
     2701 aagcgccaca atcgcttcat cgcccacatc atggccccag gtatcgttga tgctcttgaa
     2761 atggtcgata tcgataatca gtaacgttgc atcgcgatta tgccgccgac agttatcaaa
     2821 ttcattgcgt aacatagttt cccaatgacg tcggttatac acgcccgtca tgccgtcgcg
     2881 ggtactcatg acctgcaacc tgcgtttatg ttccgccagt ttggttgccg tctggtagct
     2941 gacccagcca aacagcagag gataaatgac aataatggga agggagagcc accattccag
     3001 cggcgcacta ttgaacgaca cggtaatgcc cgtcagctcg agggtgacaa ggcaggaaac
     3061 caccatcaac accagacccg cgacaaacag acgggggccg cctgccccca tcaaattcag
     3121 acacataatc atcaacatcg cggtggaagg cagcacgttt acgcccatta cgcctaccca
     3181 cattcccgct aatactgcat cggtttttaa gttgtaaatt tcccggctaa gcggatcgac
     3241 ggccctgctc gctatctgcc aggctaaatg cggccagacg aacgcccagc cgaccaacac
     3301 cagccaccac cagcccggcg gcgggtgtga aaccagcgtt gaagcaatcg gtaagaacat
     3361 gccagccagg ccaaccgcac ggggtagtct gacgcgacgg gcgaagcgca gcccggaccg
     3421 ctgatgttcg ttttgaggag ttaaaggagg ttcctccccg tgcgccgccg cttttttgaa
     3481 aaagttttca tcattcatta tttttgggaa cattcagaaa caattttccc aaattataga
     3541 gacggggcta aaggcagaag tatgaaattt cgggtgagcc attttagtag ctcacccgtt
     3601 agcactatca ggtatcaggc ggctttcttg aggcaggcgc tcataaactt attacgatca
     3661 tcacctttca gtgattgttg cgttgcttga ttattacatt cgcgcatctt ttgctgctgt
     3721 ggcgtcaaac ttttttcacc aggcgcagac ttgctgttct tcaggcaatc actcatgtag
     3781 gtcttacgag catccccttt caacgcctgt gccgtcgcct gctgattaca ggaggtcata
     3841 cgctgttgtt gtggggttaa ggttctctcg gcagcgccga cggtggttaa aaaaaccaga
     3901 ccgaaaagca aggtaaccag taatgtaatt ttcatcgcac catccctctt catgttttaa
     3961 ccatgagcgt atgcgcccgt gatctgccat taagtctggt tgctaacagc aaaaaaacca
     4021 cccggcagcg aaaattcact gccgggcgcg gttttatttc agccccaggg cggctttcat
     4081 ggtgtagaag agatcggtct ggtcggtcag tccaacaaca ttggcggcat gcgggccata
     4141 cgccgcaata cgcaactgac tgcctgtatg ttcttgtgaa tcctcttcgg agttcccgta
     4201 actcatcacc atcactgcgc catctttggt atttagcgcc tgggtgaggc ccggagcttt
     4261 ggtatccggc gcaacaatct ggctggcgtg ggcgtgatca gcggtgacta tgaccagcgt
     4321 gttaccatcc tttttggcga attccagcgc ccgttgtacg gcttcatcga gatcgaccgt
     4381 ctcgccaatt tgcccacaag gattcgcagc atgatcctgt ttatcgattg acgcaccttc
     4441 aacttgcagg aaaaagcctt tctcattttt actcaacaat tcaatggctt tgtcggtcat
     4501 ctgtgccagg gtcggtacac tgtcattacg ttgcggatta ggcgtacagg tgactgcggg
     4561 cttatcgata ttaccgtggt acgttgcttt cggtcctagc cagcgcactg gcatattgcc
     4621 gtcagcaaac aggccaagca ggggtttttg ctgattcgct tccgtcaccg aattcagtga
     4681 ggcagcatcg ctcaccaact gataaccacg cgcctgtgcc tgttcacgca gcgtttttcc
     4741 ctgccattca ccagcggttg ccgtttcagc aaaggttttt gcgccgccgc caagcgtaac
     4801 gtcggcacga gcgttaagca gctgttcggt aatcgatcct tttccgcctt tttccagagc
     4861 gttacccgga catttttcac tggtcgcgct cggaccgtag catttgcgcg aggtcacatg
     4921 tgccaccagc gcagcgggcg tggcatcctg caactctgcg gtagaaacgt taccggtcgc
     4981 cagacctgcg gcttttgcca tttccagaat cgttgggtga tctttttcgt gaatatcgac
     5041 gcccagcgcg ccgttatagg ttttgacacc ggttgaccag gcggttgctg atgcagccga
     5101 gtcggtgacg tagtccggtt tgccggtttt tttattcagc gcatagtgag tgtattgccc
     5161 ggtaagcggt aaggcatcga tacctttaaa aaagccgccc gcaccttcgg cataattacg
     5221 tgcggcagta atttccgaat cccccatccc atcgccaatc agcaaaataa tattttttgc
     5281 aggtttatcg ctaagagaat cacgcagagc ggcggtctga tcagccgtta agcggcgagc
     5341 accgccgggt gcagtaatat cgccctgagc agcccggttt tccagaacag gcatttctgg
     5401 tgtccgggct tttgtcacag gggtaaacag taacggtaag agtgccagtg caatagtgct
     5461 ttgtttcact ttattttctc catgtacaaa tacattaaaa aataaaaaca aagcgactat
     5521 aagtctcggc cgtgacaact ttatgacagc tgttgaaaag attaactttt tactgacgag
     5581 gatgcttcaa taacttcttt acgtaatcgc gcagcagctc cgtatcgtcg tcaggaatgc
     5641 tggcatcggg ctttacctcg tacagcgccc cctctacctg atcaatcaac cgctgttggt
     5701 cattttgcgc catattgcga agcattgcag tgacgataat ctccaaagct tctacctgcg
     5761 cacacagttc tttcgactct tcttcttttt gggcaagctt aaataacaac tcagcaatga
     5821 gatttttcat gtctgtattt ccttatccaa agtatggaga agttaacatt atgtttcaca
     5881 acttcatagt gtctggaagt attattttta tgacaaagga aatactt
//
LOCUS       DAPGOF010000243         5902 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.246, whole genome shotgun sequence.
ACCESSION   DAPGOF010000243 DAPGOF010000000
VERSION     DAPGOF010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.246"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..4294)
                     /locus_tag="RHV13_004408"
     CDS             complement(<1..4294)
                     /locus_tag="RHV13_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243858.1"
                     /translation="MRKSGLGLALLFSLIAPIKAVYAEAIMISGKLQADLPAVSFDPG
                     PGDFVAYVNSNTITASGAGTACNVTVDDRATSSVDNLVCFFEWLPNTLGFTSNGFTLS
                     GVPNTTGDLKLPYKISYFSGSERKKVEIVKGEYSIKSVAPVKPTITGLKSSLNGLVYD
                     GFSFKSYLKDEAIKDIAVSVEPRNYIQYISIGSGSACEVPIGGTSCTIEVGSIKASDT
                     DELLGSRDITITANSKNNYFAPPESKKLVVNWDYRPPVVDHTLWNFTDEAKTIKVGGQ
                     DIYTGAKTVAVAVKVPQQETEGEWWLPTAMSLTMTPDGVFKPTTKVTLDDGTEIDFKQ
                     SWATPLRRTLQPVSGPQKVGDEYLYIFDLTDLINGSYAATFTVENTSKNSSTYTEPES
                     KLMLSDNPTLMVLKDGQVLTKRAPVYFLNEIIVAAFQGQAGVADIKSVTIDNKVVSLT
                     PTNYRGIYYLPVGDDLAVNSDHEITVVAENLYGKNVNFSTVFTYQPTGFTLKNLEKNV
                     TLYSRVRQYTDLLSQTAGDKCTLFTTEENANAYLAWYGEKSDVTACYPQWNNVPDGLE
                     FYFKGRTPGLTGFFNKTGENLLDYQVYMINGKGSKAVSARNRRTLTTQLPYNPIISYK
                     KNKVIAGINPNTALAYTTGGEAARILAKVVPADVTMIVSQNGSEAVKTSFKNRSSNND
                     ATTFVQRVKVAAAPLWTKNVFDIAVEYSKDPELRTTDTLNVYTVPDFNIRASMEVDDR
                     KTATSLEVPLKVTVGRYNNSTRKSAFDRKTMGEWDVTIYSQKSVYGKDPETGRYKTTY
                     ERTALTEALPVNDAGIVETKIKIENMDLGNMRLVGVAKVRSPFSDFEMKRETSAVGIR
                     IYKGEELEGNLSKSLIIGRIPLSTLVSFKSASTANSDALAPTEWQQSSDNGQTWNMLS
                     DMTGKRSVSIKKTEVGKWLYRAKMTNKFTSKVSYTDALTVVTYKQPKLSIDVTDILQG
                     SDVPVTLLDNDEPIPAGTAEVLWSEDKVNWVQGDTTYTVASADTLPSTIYARMRYLDS
                     DELAEESSWKETSARLAAAKPKRLSVSVTGVSKVEVGQKVTLEGKFTNPNSKYQNGNN
                     VVEEWKTPDGQTFKGSTLSVTLTEQMLDKQGYAAFEYSAWLADNKENTVSTRRVSVKS
                     WVYKFPEMKISSKLKYDMAPTTLRVALSGIKDGDYPGVTYSREWIYDKENLVITKDEG
                     DTKEFTIENPGKYTLMVVFKDNRNNEQRIENTFVVDEQTPMNVEMTPKFSNKYMRAPL
                     DVTLRSNIKLSHSADSIDTVTYKVNGEVIPSGKNYWAQLISGLKEKKYEITIDVVSKL
                     GQRGSASVEFDVVKNAVPNCTLSYTETNLSWSFTNKCDDTDGKMVRYGWFINGELRNV
                     FGSTATLSKNLNRGKQDIKVIAYDDSGDFATQHVTVFGPAEEASKSENTVSIP"
     gene            complement(4414..4968)
                     /locus_tag="RHV13_004409"
     CDS             complement(4414..4968)
                     /locus_tag="RHV13_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000235775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer protein"
                     /protein_id="HDV1243859.1"
                     /translation="MATSDFSLKNHNVKAFGQDAALVIEMNNEDVSSSKPSPFSNEID
                     NYYLTLHVAPRNAKKDYDWGSNRSVLLKLSTNEVMQMASVFLRIMHTLKIDKRKTSHH
                     GHVVYKNISVTPNERGGLLLSAGIVPVDKDGLKPFMHMVPVSQMDCVKIGLYILGYLA
                     QKTPWVSSESIITALRLSEAKNSK"
     gene            complement(4977..5402)
                     /locus_tag="RHV13_004410"
     CDS             complement(4977..5402)
                     /locus_tag="RHV13_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="htdF"
                     /protein_id="HDV1243860.1"
                     /translation="MKSKIRYVLSGFVVICAFAGVYKILNNVPVKPDLLDFTGNTFKK
                     TSLFLPCDKSSPSLNIKIADNEKIVINGIASKVTFVEKADPVKSPGFCDDLDLNNSRL
                     VHTASYSMVISETKTGYTLSNFKHLADDESLGGMWFYQK"
     gene            complement(5392..5844)
                     /locus_tag="RHV13_004411"
     CDS             complement(5392..5844)
                     /locus_tag="RHV13_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transfer repressor"
                     /protein_id="HDV1243861.1"
                     /translation="MLSRNSLIHGLRRDQLIGVLTISEFPVVMVESHFIQSEVMGIKP
                     VIFNIDELLVSISPISSLKFDWEWAPVDTILIEVIIPPVESDLVSAENDFLRDSGIGH
                     IQCEPGGASIRRTVTFVGGITADNLLYQLRLMCVSALKLLGEELGDEV"
BASE COUNT         1615 a         1278 c         1207 g         1802 t
ORIGIN      
        1 ttggtattga tacagtgttt tcagacttac ttgcctcttc agccggtccg aaaactgtca
       61 cgtgctgcgt tgcaaagtct ccactatcat cataagcaat tacttttatg tcctgcttac
      121 cacggttaag gtttttagaa agtgtagccg tactaccgaa aacgttcctt aattcgccgt
      181 tgatgaacca tccatatcta accatttttc catcagtatc atcacattta ttggtaaagc
      241 tccagcttag gttggtttcc gtgtagctca atgtacaatt aggcactgcg tttttaacga
      301 catcaaactc aacagatgct gaacctctct gtccaagttt actaacaaca tcaattgtga
      361 tctcgtattt cttttctttt aaacccgaaa tcagttgagc ccaataattt ttgccgctgg
      421 gtattacctc accgttcacc ttgtatgtga ccgtatctat cgagtccgcc gaatgagata
      481 gcttgatgtt tgaccttaag gttacatcga gtggagctct catatatttg ttagagaact
      541 tgggtgtcat ctcaacgttc attggcgttt gttcatcgac aacaaaggta ttttcgatac
      601 gctgttcatt gttgcggtta tctttgaaaa caaccatcaa tgtatatttg ccaggatttt
      661 caattgtaaa ttctttggtg tcaccttcat ctttagtgat taccagattc tctttgtcgt
      721 aaatccattc tcgggaataa gtaacaccag gatagtctcc atccttaata ccgctcaacg
      781 caacgcgtag ggtggtcggt gccatgtcgt atttaagttt ggagcttatt ttcatttcgg
      841 ggaatttata aacccacgat ttgacagaaa ctctacgggt agaaaccgta ttctccttat
      901 tgtccgctaa ccatgcactg tattcaaaag cagcatatcc ctgtttatcc aacatttgct
      961 cagtaagggt aacagacagt gtagagcctt taaatgtctg gccatccggt gttttccatt
     1021 cttcaactac gttattacca ttctggtact tactgttagg gttcgtgaac ttaccttcca
     1081 aagtaacttt ttgaccaacc tctaccttag aaacccccgt taccgagaca gaaaggcgct
     1141 tgggttttgc ggctgccaac cgagcagacg tttccttcca tgaagactcc tctgccagtt
     1201 catcggaatc aaggtaacgc atccgggcat aaatcgtaga cggtagggta tcagctgaag
     1261 ccacagtgta agttgtatct ccctgcaccc agttgacttt atcctctgac cagagaacct
     1321 cagcggtgcc tgctggtata ggctcatcgt tatcaagcag ggttacaggt acatctgagc
     1381 cctgaagaat gtctgtcaca tctatactga gcttaggctg cttgtaggta actaccgtta
     1441 aggcatctgt gtaggaaacc ttagatgtga atttattggt catttttgcc ctataaagcc
     1501 actttcctac ttctgtcttt ttgatactta cacttctctt tccggtcatg tcggaaagca
     1561 tattccaggt ttgaccatta tcagaggact gctgccattc ggtgggggca agtgcgtcag
     1621 agttagcagt actggcagat ttgaagctta ccagagtact aagtgggatc cttccgataa
     1681 taaggctttt cgacaggtta ccctccagtt cctcaccttt atagattcga atgcctacag
     1741 cactggtctc acgtttcatt tcaaaatcag agaatggcga tctgacctta gccacaccaa
     1801 ccagacgcat attacccaga tccatatttt caattttaat cttggtttcg acaatgcccg
     1861 catcattgac cggcaatgct tcagtcagcg cagttctttc gtatgtagtt ttataacggc
     1921 ctgtttctgg atctttgccg tatacacttt tttgcgagta tatcgtgacg tcccattctc
     1981 ccattgtttt acggtcaaat gcacttttac gtgtggagtt gttataacgc cccactgtta
     2041 cctttaaggg cacttcaaga gatgtggctg tttttctgtc atccacttcc atcgatgctc
     2101 gaatattgaa atcaggcacc gtatatacgt tgagtgtgtc tgtagttctt aattctggat
     2161 cctttgagta ttctacagct atgtcaaaga cgttctttgt ccacaatggt gcagcagcaa
     2221 ccttaaccct ttgtacaaag gtcgttgcat cgttgtttga ggaacgattt ttaaatgacg
     2281 ttttgacagc ctcagagccg ttttgagaga cgatcatcgt aacgtcagct ggtaccacct
     2341 tcgctaatat tcgagcggcc tcccccccgg tcgtataagc cagagcagtg ttcggattga
     2401 tgccagctat gactttattc tttttgtaag agatgatcgg gttgtatggt aattgtgtgg
     2461 tcagagtacg gcgattcctt gcggataccg ctttagagcc cttcccgtta atcatataga
     2521 cttgatagtc gagcagattc tcacctgttt tattaaagaa tccagttaga cccggggtac
     2581 gtcctttaaa atagaactcc aacccatcag ggacattgtt ccactgaggg tagcatgctg
     2641 taacatctga tttttcccca taccaggcga ggtaagcatt cgcattttcc tctgtggtaa
     2701 acagagtgca tttatcacca gctgtttgac taagcaggtc cgtatactga cgtacacgcg
     2761 agtaaagagt tacgtttttt tccagattct tgagagtgaa tccagtaggt tgataagtga
     2821 aaacggtact gaaattaacg ttttttccat aaaggttttc agccaccaca gtaatctcgt
     2881 gatccgaatt aactgctaaa tcgtctccaa ccggcaggta gtaaatacct ctataatttg
     2941 taggtgttaa cgaaactact ttgttgtcta ttgttactga ttttatgtca gcaaccccag
     3001 cctgcccctg gaaagctgca acgataatct cgttcaggaa gtatacgggt gctcgtttag
     3061 ttaatacctg cccatctttg agaaccataa gcgttgggtt atcagacagc atcagtttgc
     3121 tttcaggctc agtgtacgta gaagagtttt tgctcgtatt ttctactgta aaagtcgctg
     3181 cgtaggaccc gtttataaga tccgttaagt cgaagatata gaggtactca tcaccgactt
     3241 tttgaggtcc acttaccggc tgtaaagttc tacgtaaagg ggttgcccag gactgtttga
     3301 aatcaatctc agtgccatca tctaaagtca ctttagtcgt cggtttaaac acgccatccg
     3361 gagtcatagt gagcgacata gctgttggga gccaccattc accttctgtt tcttgctggg
     3421 gtaccttaac ggccacagca acggttttgg ctccggtata gatgtcctgg ccacctactt
     3481 tgattgtttt agcctcatcg gtaaaattcc aaagtgtatg gtctactaca ggaggccgat
     3541 aatcccagtt aacaacaagc tttttggatt caggaggtgc gaaataatta ttcttagagt
     3601 ttgctgtaat tgtgatatca cgtgaaccca aaagttcatc tgtatcactg gccttaatgc
     3661 ttccaacttc aatggtacag gaagttccgc caataggaac ttcacaggcg gagcctgaac
     3721 ctattgagat gtattgaata taattgcggg gctcaacaga aacagcgata tctttgatcg
     3781 cttcatcctt taaatacgat ttgaaagaaa agccgtcata gactaatccg ttcagggatg
     3841 atttaagacc ggtgatagta ggttttaccg gtgccacact tttgattgaa tattcacctt
     3901 ttactatttc aacttttttc cgttcagaac cagagaagta agaaatcttg taaggtagtt
     3961 ttaaatcacc ggtggtgttg ggaacgcctg aaagagtgaa accgttagaa gtaaatccca
     4021 aggtattggg taaccactca aaaaaacaaa cgaggttatc aacagaggaa gtcgctcgat
     4081 catcaaccgt tacattacag gctgtgcctg cacctgaggc agtgatagtg ttagaattaa
     4141 cgtatgcaac aaagtcgccc ggccccgggt caaaggatac ggctggcaaa tcagcctgta
     4201 gctttcctga aatcataatc gcttcggcat agaccgcttt aatcggagca attaaagaaa
     4261 acaacagggc taaacctaaa cctgattttc tcatgacgaa tacctgtttt tctgagcaaa
     4321 attaaactca ctttacgatg aaaaacagaa atatccgttc gtataacatt taaaaaaacc
     4381 tgtatttttc catgtattcc gtatgttaat acgctatttt gaattcttgg cttcagataa
     4441 gcgaagcgcg gtaattatag actcggagct tacccaaggc gttttttgag caagatatcc
     4501 aagtatgtag aggccaattt taacgcagtc catttgtgag acggggacca tgtgcatgaa
     4561 gggttttaag ccgtctttat cgactggaac tatccctgct gataacaaca aaccgcccct
     4621 ctcgtttggg gtaacgctta tatttttgta tacgacgtgg ccatgatgtg aggttttcct
     4681 cttatcaatt tttaaagtat gcatgattct aagaaataca gatgccatct gcataacttc
     4741 attcgtcgac aattttagaa gtacagaacg gtttgagccc cagtcataat cttttttggc
     4801 gttcctcgga gcgacgtgca atgtaagata ataattatca atctcatttg aaaaaggtga
     4861 aggttttgat gagctaacat cttcattgtt catctcaatg acaagtgctg catcctgacc
     4921 aaaagctttt acattatggt ttttaagaga gaagtctgaa gttgccatat aaaaagtcac
     4981 ttttgataaa accacatacc acctaatgat tcatcatcgg caagatgctt gaaatttgag
     5041 agtgtatatc ctgttttggt ttcagaaatc accatgctgt aagatgccgt atgtacaagg
     5101 cgagaattgt ttagatcaag atcatcacaa aaaccagggc ttttgacggg gtcagccttt
     5161 tcaacaaaag ttacttttga agcgatgccg ttgatcacga ttttctcgtt atcagcaatt
     5221 ttaatgttta aagaaggcga gcttttatca catggcagga agagtgatgt ctttttaaat
     5281 gtgtttccag tgaagtccaa aaggtcaggt ttaaccggaa cattgtttag gattttataa
     5341 actccagcaa atgcacatat cacaacaaaa ccagataaaa catatctaat cttagacttc
     5401 atcccccagt tcctctccta aaagttttaa cgcacttacg cacataagcc tcaactgata
     5461 cagtaagtta tcagcggtta ttccaccaac gaaggtaact gtacgtcgta ttgatgctcc
     5521 gccaggctcg cactggatat ggccgatacc agaatcacgg aggaagtcat tttctgcact
     5581 tacaaggtct gactctacag gtggaatgat aacttcaatg agtattgtat caactggggc
     5641 ccactcccaa tcgaacttaa gagaggaaat tggggatata gaaacaagaa gctcatcaat
     5701 attaaaaatt acgggtttta ttcccataac ctcagactga atgaagtggc tttctaccat
     5761 aactaccgga aactctgaaa tagtcagaac tccgattaac tggtctcggc gtaaaccatg
     5821 tattagtgag ttgcgagata gcattcgatc agcctttatt ataataatca aacatttggg
     5881 acaaggacaa aagggattac ag
//
LOCUS       DAPGOF010000244         5739 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.247, whole genome shotgun sequence.
ACCESSION   DAPGOF010000244 DAPGOF010000000
VERSION     DAPGOF010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.247"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..789
                     /locus_tag="RHV13_004412"
     CDS             <1..789
                     /locus_tag="RHV13_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV1243862.1"
                     /translation="SYAGVHSNINYYDLNPNLDVGVAIDILGVGYVNAPFDYHANKPS
                     GGTKYKCNRVEPLNVSSGAKAVVYFYIKKTFTGKLIIPETKIVTLYGTISRDTPVDYS
                     QPMADVYIRGDITAPQSCEINNLQPVYFDFKEIPAADFSSVAGSTVTTHKITKTVTIE
                     CENLGILNTDDISTSFYATEPNTDNSMVVTSNPNVGIKIYDKNNNEIKVNGGELPTDM
                     GKSTVYGEKSGSVTFSAVPASLTGARPAPGSFTATATITIEIVR"
     gene            1038..2078
                     /gene="pstS"
                     /locus_tag="RHV13_004413"
     CDS             1038..2078
                     /gene="pstS"
                     /locus_tag="RHV13_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDV1243863.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            2165..3124
                     /gene="pstC"
                     /locus_tag="RHV13_004414"
     CDS             2165..3124
                     /gene="pstC"
                     /locus_tag="RHV13_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HDV1243864.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            3124..4014
                     /gene="pstA"
                     /locus_tag="RHV13_004415"
     CDS             3124..4014
                     /gene="pstA"
                     /locus_tag="RHV13_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDV1243865.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFTEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            4105..4878
                     /gene="pstB"
                     /locus_tag="RHV13_004416"
     CDS             4105..4878
                     /gene="pstB"
                     /locus_tag="RHV13_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HDV1243866.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            4893..5618
                     /gene="phoU"
                     /locus_tag="RHV13_004417"
     CDS             4893..5618
                     /gene="phoU"
                     /locus_tag="RHV13_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDV1243867.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDPKE"
BASE COUNT         1403 a         1398 c         1514 g         1424 t
ORIGIN      
        1 agttatgctg gggttcattc taatataaat tactatgacc ttaatccgaa tcttgatgtg
       61 ggggttgcta tagatattct tggtgttggg tatgttaatg cgccttttga ttatcatgct
      121 aacaaaccgt ctggaggtac aaagtacaag tgcaaccgag tagaaccttt aaatgtatcc
      181 agtggcgcca aagcggtagt atacttttat atcaagaaga catttacagg aaaattgatt
      241 attcctgaaa caaagatagt gacattgtat ggaaccataa gccgtgacac cccggtagat
      301 tactcacagc cgatggctga tgtgtatatt cgtggcgata ttacagcgcc gcaaagttgt
      361 gaaattaata atttacagcc agtttatttt gattttaaag aaataccagc tgcggatttt
      421 tcatctgttg cgggtagtac ggtaacaaca cataaaatca ctaaaacggt cactattgag
      481 tgtgaaaacc tggggatact gaataccgat gatatcagta cgtcttttta tgctactgaa
      541 cccaatacgg ataactcgat ggtcgtaaca tcaaacccta acgtggggat aaaaatttac
      601 gacaaaaata ataacgaaat caaagtgaac ggtggcgaac tgccgactga tatggggaaa
      661 tcaacggtct acggagagaa atccgggagt gtcacgtttt ctgccgttcc cgcaagcctt
      721 actggcgctc gccccgctcc aggatcattt actgcgactg caacgataac gattgagata
      781 gtacgctaac cgaactgaca acggcgctcc tcacgccgtt gtcatcttaa gcattttccc
      841 cagcgcattt ttacgcatca tgacaaaaaa atgacatcac ttatcttttt gttcattcac
      901 aaaaatcaat ggattacgca atagttttgt tttgtcataa aactgtcata aaccttacat
      961 ataactgtca cctgtttgtc ctattttgct tctcgtagcc aacaaacaat gctttatgaa
     1021 tcctcccagg agacattatg aaagttatgc gtaccaccgt cgcaactgtt gtcgccgcga
     1081 ccttatcgat gagtgctttc tctgtgtttg cagaagcaag cctgacaggt gcaggtgcaa
     1141 ccttccctgc gccggtgtat gccaaatggg ctgacactta ccagaaagaa accggtaata
     1201 aagttaacta ccagggtatc ggttcttccg gtggcgtaaa acagattatc gctaataccg
     1261 ttgattttgg tgcctctgac gcgccgctgt ctgacgaaaa actggctcag gaaggcctgt
     1321 tccagttccc gaccgtgatt ggcggcgtgg tgctggcggt taacattcca gggttgaagt
     1381 ctggcgagct ggtgctggat ggtaaaaccc tcggcgacat ctacctcggc aaaatcaaga
     1441 agtgggatga tgaagccatc gccaaactga atccgggtct gaaactgcct tcacagaaca
     1501 ttgctgtagt acgccgcgca gatggctccg ggacttcctt cgtcttcacc agctacctgg
     1561 cgaaagtgaa tgaagagtgg aaaaacaacg ttggtactgg ctctaccgta aaatggccga
     1621 tcggtctggg tggtaaaggt aacgacggta tcgctgcgtt cgttcagcgt ctgccgggtg
     1681 caattggtta cgttgaatat gcttacgcta agcagaacaa cctggcgtat accaaattga
     1741 tctccgctga tggtaaaccg gttagtccga ccgaagaaaa cttcgctaat gcagcaaaag
     1801 gcgcagactg gagcaaaacc ttcgctcagg atctgaccaa ccagaaaggc gaagatgcgt
     1861 ggcctattac ctctaccacg ttcattctga tccacaaaga tcagaagaaa ccagaacaag
     1921 gcacagaagt gctgaagttc ttcgactggg cttacaaaac cggggctaaa caggcgaacg
     1981 acctggatta cgccagcctg ccggatagcg tagttgaaca ggttcgcgct gcgtggaaga
     2041 ccaatattaa agacagtagc ggcaagccgc tgtactaata aaactccagg ccggataagg
     2101 cattaaacgg cttatccggc attacaaaat gactttgtaa acgcgtttaa ctgaagagta
     2161 acttatggct gcaaccaagc ctgcttttaa cccaccgggt aaaaagggcg acataatttt
     2221 cagcgtgctg gtaaaactgg cggcgctgat tgtgctattg atgttgggtg gcattattgt
     2281 ctctctgatc atctcctcct ggccgagcat tcagaaattt ggtctggctt tcctctggac
     2341 caaagagtgg gatgcaccga acgatatcta cggggcgctg gtgccgatct acggtacgtt
     2401 ggtgacttcg tttatcgcgc tgctgatcgc cgtcccggtg agtttcggta tcgccctgtt
     2461 cctgactgag cttgcgcctg gctggctgaa acgcccgctg ggtatcgcca ttgagctgct
     2521 ggcagccatt ccaagtatcg tttacggcat gtggggcctg tttatctttg cgccgctgtt
     2581 cgccgtttac ttccaggagc cggtcggcaa tatcatgtcg aatatcccga ttgtcggcgc
     2641 gctgttctct ggcccggcat ttggtatcgg tatcctcgcg gcaggcgtga tcctcgccat
     2701 catgattatt ccgtacattg cggcggtaat gcgcgatgtg ttcgaacaaa ccccggtgat
     2761 gatgaaagag tcggcctacg gtattggctg caccacctgg gaggttatct ggcgtatcgt
     2821 tcttccgttt accaaaaatg gtgttatcgg cggcattatg ctggggctgg gccgcgcgct
     2881 tggtgaaacc atggcggtga cctttatcat cggtaacacc taccagctcg acagcgcctc
     2941 gctgtacatg ccgggcaaca gtatcacctc tgcgctggcg aacgaatttg ccgaagcgga
     3001 atccggtctg cacgttgctg cactgatgga actgggcctg atcctgtttg tgattacctt
     3061 catcgtcctc gccgcatcga agtttatgat tatgcgcctg gctaagaatg agggggctcg
     3121 ctaatggcta tggttgaaat gcaaaccact gcggcgctgg cggaatcccg ccgcaaaatg
     3181 caggcgcgtc gccgcctcaa aaaccgtatt gcgctgacgc tctcgatggc gacgatggcc
     3241 ttcggtctgt tctggctgat ctggatttta atgtccacca tcacccgcgg tatcgacggt
     3301 atgtcgctgg cgctgtttac tgaaatgacg ccgccgccca atacggaagg tggtggtctg
     3361 gcgaacgctc tggcgggtag cgggctgtta attttgtggg ccacggtatt cggtactccg
     3421 ctgggcatta tggcggggat ttatctggct gaatatggac gtaaatcctg gctggcagaa
     3481 gtgattcgct tcattaacga cattctgctc tctgcgccgt cgattgtggt tggtctgttt
     3541 gtttacacca ttgtggtggc gcagatggag cacttctccg gttgggcggg tgtgattgcc
     3601 ctggcgctgt tgcaggtacc gattgttatc cgaaccaccg agaacatgct gaaactggtg
     3661 ccggacagcc tgcgtgaagc ggcttatgcg ctgggtacac cgaagtggaa gatgatctcc
     3721 gcgattacgc tgaaagcgtc ggtgtccggg atcatgaccg gtatcctgct ggcgattgcc
     3781 cgtattgctg gtgaaaccgc gccgctgctg tttaccgcgc tctccaacca gttctggagc
     3841 acggacatga tgcaaccgat cgccaacctg ccggtaacga tctttaagtt tgcgatgagc
     3901 ccgtttacgg aatggcagca actggcctgg gccggggtat tgatcattac cctgtgcgta
     3961 ctgctgctga acattctggc gcgcgttgtt tttgcgaaga ataaacacgg ttaacacttt
     4021 gccggatgcg gcgtaaacgc ctgatccggc ctacggtaag cctgattagc gaagtgcatc
     4081 aggcacgatg aggaaaagat tgcaatgagt atggttgaaa ctgccccgag taaaattcag
     4141 gttcgtaatt tgaacttcta ctacggcaaa ttccatgccc tgaaaaacat caacctggat
     4201 atcgccaaaa accaggttac ggcgtttatc gggccgtccg gctgcggtaa atcgacgctg
     4261 ctgcgtacct tcaataaaat gtttgaactg tacccggagc agcgtgcgga aggtgaaatt
     4321 ctgcttgatg gcgacaacat cctgaccaac tctcaggata tcgccctgct gcgtgcgaaa
     4381 gtgggcatgg tgttccagaa accgacgccg ttcccgatgt ccatctacga caacatcgcc
     4441 tttggtgtgc gtctgtttga gaagctctcc cgtgccgaca tggacgagcg cgtgcagtgg
     4501 gcattgacca aagccgcatt gtggaacgaa accaaagata aattgcatca gagcggttac
     4561 tctctctctg gtggtcagca acagcgtctg tgcattgcgc gtggtatcgc cattcgcccg
     4621 gaagtgctgc tgctcgacga accatgttcg gcgctcgacc ccatctccac cgggcgtatt
     4681 gaagagctga tcaccgaact gaagcaggat tacaccgtgg tgatcgtcac ccacaacatg
     4741 cagcaggctg cgcgttgttc cgaccacacg gcgtttatgt acctgggcga attgattgag
     4801 ttcagcaaca cggacgatct gttcaccaag ccagcgaaga aacaaacaga agactacatc
     4861 accggtcgtt acggttgatt caggagtgcg ttatggacag tctcaatctt aataaacata
     4921 tttccggcca gttcaacgcc gaactggaaa gtatccgcac gcaggtgatg accatgggcg
     4981 gcatggtgga gcagcagctt tctgatgcaa tcaccgcgat gcataaccag gacagcgatc
     5041 tggcgaagcg cgtgatcgaa ggcgacaaga acgtcaacat gatggaagtg gcgatcgatg
     5101 aagcctgcgt gcgcattatt gccaaacgtc agccgacagc gagcgacctg cgtctggtta
     5161 tggtgatcag taaaaccatt gctgaactgg agcgtattgg cgacgtggcg gacaaaatct
     5221 gccgtactgc gctggagaaa ttctcccagc agcatcagcc gttgctggta agtctggagt
     5281 cgctgggccg tcatactatc cagatgctgc acgacgtgct ggacgcgttc gcgcggatgg
     5341 acattgatga agcggtacgt atttatcgtg aagataaaaa agtcgatcag gagtatgaag
     5401 gtattgttcg tcaactgatg acctacatga tggaagattc gcgtaccatt ccgagcgtac
     5461 ttaccgcgct attctgtgcg cgttctatcg aacgtattgg cgaccgctgc cagaatattt
     5521 gtgagtttat cttctactac gtgaagggtc aggatttccg tcatgtaggc ggtgatgagc
     5581 tggataaact gctggcggga aaagacccga aagagtagtg ctaactgtag gccagataaa
     5641 gctttttgcc gcatctggca atcagcccgg tcatacatag ttaccgggct gatacacttt
     5701 ctccctgaaa tatagccaca caaactcacc tcgaaacta
//
LOCUS       DAPGOF010000245         5723 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.248, whole genome shotgun sequence.
ACCESSION   DAPGOF010000245 DAPGOF010000000
VERSION     DAPGOF010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5723
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.248"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            7..996
                     /gene="ldhA"
                     /locus_tag="RHV13_004418"
     CDS             7..996
                     /gene="ldhA"
                     /locus_tag="RHV13_004418"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDV1243868.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            1107..1529
                     /gene="hslJ"
                     /locus_tag="RHV13_004419"
     CDS             1107..1529
                     /gene="hslJ"
                     /locus_tag="RHV13_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HDV1243869.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTGDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(1526..1792)
                     /locus_tag="RHV13_004420"
     CDS             complement(1526..1792)
                     /locus_tag="RHV13_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HDV1243870.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            2066..5590
                     /gene="nifJ"
                     /locus_tag="RHV13_004421"
     CDS             2066..5590
                     /gene="nifJ"
                     /locus_tag="RHV13_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDV1243871.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNSWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAIFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPYS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         1421 a         1474 c         1483 g         1345 t
ORIGIN      
        1 agtcttatga aactcgccgt ttatagcaca aaacagtacg acaagaagta cctgcaacag
       61 gtgaacgagt cctttggctt tgagctggaa ttttttgact ttctgctgac ggaaaaaacc
      121 gctaaaactg ccaatggctg cgaagcggta tgtattttcg taaacgatga cggcagccgc
      181 ccggtgctgg aagagctgaa aaagcacggc gttaaatata tcgccctgcg ctgtgccggt
      241 ttcaataacg tcgaccttga cgcggcaaaa gaactggggc tgaaagtagt ccgtgttcca
      301 gcctatgatc cagaggccgt tgctgaacac gccatcggta tgatgatgac gctgaaccgc
      361 cgtattcacc gcgcgtatca gcgtacccgt gacgctaact tctctctgga aggtctgacc
      421 ggctttacta tgtatggcaa aacggcaggc gttatcggta ccggtaaaat cggtgtggcg
      481 atgctgcgca ttctgaaagg ttttggtatg cgtctgctgg cgttcgatcc gtatccaagt
      541 gcagcggcgc tggaactcgg tgtggagtat gtcgatctgc caaccctgtt ctctgaatca
      601 gacgttatct ctctgcactg cccgctgaca ccggaaaact accatctgtt gaacgaagcc
      661 gccttcgatc agatgaaaaa tggcgtgatg atcgtcaata ccagtcgcgg tgcattgatt
      721 gattctcagg cagcaattga agcgctgaaa aatcagaaaa ttggttcgtt gggtatggac
      781 gtgtatgaga acgaacgcga tctgttcttt gaagataaat ccaacgacgt gatccaggat
      841 gacgtattcc gtcgcctgtc tgcctgccac aacgtgctgt ttaccggaca ccaggcattc
      901 ctgacagcag aagctctgac cagtatttct cagactacgc tgcaaaactt aagcaatctg
      961 gaaaaaggtg aaacctgccc gaacgaactg gtttaatctt gccgctcccc tgcaatccag
     1021 gggagctgat tcagataatc cccaatgacc tttcattctc tattcttaaa atagtcctga
     1081 gtcagaaact gtaattgaga accacaatga agaaagtagc cgcgtttgtt gcgctaagcc
     1141 tgctgatggc gggatgtgta agtaacgaca aaattgctgt tacgccagaa cagctacagc
     1201 atcatcgctt tgtgctggaa agcgtaaacg gtaagcccgt gaccggcgat aaaaatccgc
     1261 cagaaatcag ctttggtgaa aaaatgatga tttccggcag catgtgtaac cgctttagcg
     1321 gtgaaggcaa actgtctaat ggtgaactga cagccaaagg gctggcaatg acccgtatga
     1381 tgtgcgctaa cccgcagctt aatgaactcg ataacaccat tagcgaaatg ctgaaagaag
     1441 gtgcacaagt ggatctgacc gcgaaccagt taacgctggc gaccgcgaaa cagacattaa
     1501 cttataagct ggcggattta atgaattaat agctgccaca gctcccggcg gcaagtgact
     1561 gttcgctaca gcgtttgccg ttgggtaatg cacacatccc aatcgccgta ccatccagtt
     1621 gacgggcaac agaaagcgaa ccgccgatca ttgcacaatt tgcttctcca ctactggaca
     1681 tcgacgcttt taaacctggc gctacgtgcg ccgcagtcgc ctgctgaaca ggctcactac
     1741 tacacgccga caacaataaa gcggcacacc ctacccaaaa cgctgctcgc atcttttctt
     1801 cctctaatct tcaagccaaa cgacaccgcc ataaataata ggcagcacag agggcggcgt
     1861 cgagagctgt cctgcgcgtt gccccgcatt tttacttttt tatggctatt tttttgccct
     1921 ctgtttgatc aaaacattca ttactctgat gtgggggaca caaaagcgaa aatgcagaag
     1981 aaagccattt gctaaaattg aaggattact actgggcgcg cagcaatttc gtgcgcccct
     2041 catttgcgca atgtaagggt gtcatatgat tactattgac ggtaatggcg cggttgcttc
     2101 ggtggcgttt cgcaccagtg aagttatcgc catctaccct attaccccta gttccacgat
     2161 ggcagaacag gctgatgcct gggccggaaa cggcttgaag aacgtttggg gagacacacc
     2221 acgcgtggtt gaaatgcagt cggaagcggg tgctatcgct accgtgcatg gcgctttgca
     2281 gacgggtgcc ctttcaacat cgtttacgtc atcgcagggt ttgctgctga tgatcccgac
     2341 gctgtacaaa ctggcaggcg aactcacacc gtttgtcctg catgtagcgg cacgtaccgt
     2401 tgccacacat gcactctcta tttttggcga tcattccgac gtcatggcgg tgcgccagac
     2461 gggttgcgcg atgttgtgtg cggcaaacgt ccaggaagcg caagactttg ctctcatttc
     2521 gcacatcgcg acgctgaaaa gccgcgtgcc atttattcat ttctttgatg gtttccgtac
     2581 gtcccacgaa atcaacaaaa ttgtcccgct ggccgatgac actattcttg atctcatgcc
     2641 gcaggccgaa attgatgctc atcgcgcccg ggcgctcaac ccggaacatc cggtgatccg
     2701 cggtacgtcc gccaatcctg acacttattt ccagtctcgc gaagccacca actcatggta
     2761 caacgcggtc tatgaccatg ttgaacaggc gatgaatgat ttcgctgccg cgacaggtcg
     2821 tcagtatcag ccgtttgagt attacgggca tccgcaagcg gaacgggtga ttatcctgat
     2881 gggctctgcc attggcacct gtgaagaagt ggttgatgaa ttgctaaccc gtggcgaaaa
     2941 agttggcgtg ctgaaagttc gcctgtaccg tcccttctcc gccaaacact tgctgcaagc
     3001 tctgccggga tccgtacgca gcgtggcggt actggacaga accaaagaac ccggtgccca
     3061 ggcagaaccg ctctatctgg atgtaatgac cgcactggca gaagccttta ataatggcga
     3121 gcgcgaaact ctgccccgtg tcattggtgg gcgctatggt ctttcatcca aagaatttgg
     3181 cccggactgt gtactggcga tatttgccga gctcaacgcg gctaaaccga aagcgcgctt
     3241 tacggttggt atttacgatg atgtgaccaa tctgtcactg ccgttgccgg aaaacaccct
     3301 gccaaactcg gcgaaactgg aagccttgtt ttatggcctt ggtagtgatg gcagcgtttc
     3361 cgcgaccaaa aacaatatca agattatcgg taattccacg ccgtggtacg cacagggcta
     3421 ttttgtttac gactccaaaa aggcgggcgg cctgacggtt tctcaccttc gcgtcagcga
     3481 acagccgatt cgttccgctt atctcatttc ccaggctgat tttgttggct gccaccagtt
     3541 gcagtttatc gataaatatc agatggctga gcgtttaaaa cctggcggca ttttcctgct
     3601 caacacgccg tacagcgcag atgaagtgtg gtcgcgcttg ccgcaagaag ttcaggccgt
     3661 gttaaaccag aaaaaagcgc gcttctatgt gattaacgca gcgaaaatcg cccgcgaatg
     3721 tggcctggcg gcccgtatta ataccgtcat gcagatggct tttttccatc tgacgcaaat
     3781 tctgcctggc gatagcgccc tcgcagaatt gcagggtgcg attgccaaaa gttacagtag
     3841 caaaggtcag gatctggtgg agcgcaactg gcaggctctg gcgctggcgc gtgaatccgt
     3901 agaagaagtt ccgttgcagc cggttaatcc gtacagcgcc aatcgaccgc cagtggtttc
     3961 cgatgccgcc cctgattttg tgaaaaccgt aaccgctgcg atgctcgccg ggcttggtga
     4021 tgccctcccc gtttcggcgc tgccgccaga cggcacctgg ccgatgggca ctacgcgctg
     4081 ggaaaaacgc aatatcgccg aagagatccc catctggaaa gaggaactct gtacccaatg
     4141 taaccactgc gttgccgctt gcccacactc agctattcgc gcaaaagtgg tgccgcctga
     4201 agcgatggaa aacgcccctg ccagcctgca ttcgctggat gtgaaatcgc gtgatatgcg
     4261 cgggcagaaa tatgtcttgc aggtggcacc ggaagattgc accggttgta acctgtgcgt
     4321 cgaagtttgc ccggcgaaag atcgtcagaa tccagagatt aaagccatca atatgatgtc
     4381 tcgcctggaa catgtcgaag aagagaaaat caattacgat ttcttcctca acctgccaga
     4441 aatcgaccgt agcaaactgg aacgtattga tattcgtaca tcgcagctga ttacaccgct
     4501 gtttgaatat tcaggtgctt gctccggttg tggcgagacg ccgtatatta aattgctgac
     4561 tcagctctat ggcgaccgga tgttgatcgc taacgccacc ggctgttctt caatttatgg
     4621 cggtaacctg ccctctacac cgtataccac cgatgccaac ggtcgtgggc cggcatgggc
     4681 gaactctctg tttgaagata atgccgaatt tggccttggt ttccgcctga cggtcgatca
     4741 acaccgtgtc cgcgtgctgc gtctgctgga tcaatttgcc gataaaatcc cggcggaatt
     4801 actgacggcg ttgaaatcag acgctacgcc agaggttcgt cgagaacagg tcgcagcttt
     4861 acgccagcaa ctcaacgatg ttgccgaagc acatgaactg ctgcgtgacg cagatgcact
     4921 ggtagaaaaa tcaatctggc tgattggtgg cgacggctgg gcttacgata tcggctttgg
     4981 cggtctggat catgtattga gtttgacgga aaacgtcaac attctggtgc tggatacgca
     5041 atgctattcc aacaccggtg gccaggcgtc gaaagcgaca ccgctgggtg cagtaactaa
     5101 atttggcgag cacggcaaac gtaaagcgcg taaagatctt ggcgtcagta tgatgatgta
     5161 cggtcatgtt tatgtggcgc agatttctct cggcgcgcag ctcaaccaga cggtgaaagc
     5221 gattcaggaa gcggaagcgt atccggggcc atcgctgatc atcgcttata gcccatgtga
     5281 agagcatggt tacgatctgg cactcagcca cgaccagatg cgccaactca cagctaccgg
     5341 cttctggccg ctatatcgct ttgatccgcg tcgtgccgat gaaggcaaac tgccgctggc
     5401 cttggattca cgcccgccgt cagaagcact ggaagaaacg ttacttcatg agcaacgttt
     5461 ccgtcgtctg aattcacagc agccagaagt ggcagaacag ttatggaaag atgctgcagc
     5521 tgatttgcaa aaacgctatg acttcctggc acaaatggcc ggaaaagcgg aaaaaagcaa
     5581 caccgattaa atgctctgga taaggattat ccaattctaa aaaaaagccc ggacgactgt
     5641 tcgggcttgt ctttttatat gttggaaaat cagtggcaat gcaatggccc aacagaaaat
     5701 aaaatctgtg acaataaagg cat
//
LOCUS       DAPGOF010000246         5638 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.249, whole genome shotgun sequence.
ACCESSION   DAPGOF010000246 DAPGOF010000000
VERSION     DAPGOF010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.249"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(58..915)
                     /locus_tag="RHV13_004422"
     CDS             complement(58..915)
                     /locus_tag="RHV13_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV1243872.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRV
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMKNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRG
                     LYASFLYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNEDFVEKDSNDVVC
                     EIFIPVRPV"
     gene            1163..2032
                     /locus_tag="RHV13_004423"
     CDS             1163..2032
                     /locus_tag="RHV13_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDV1243873.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTREELFITSKLWVQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKAFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHN"
     gene            complement(2192..2785)
                     /gene="rclC"
                     /locus_tag="RHV13_004424"
     CDS             complement(2192..2785)
                     /gene="rclC"
                     /locus_tag="RHV13_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HDV1243874.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(2797..3033)
                     /locus_tag="RHV13_004425"
     CDS             complement(2797..3033)
                     /locus_tag="RHV13_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDV1243875.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     DTIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            complement(3142..4467)
                     /locus_tag="RHV13_004426"
     CDS             complement(3142..4467)
                     /locus_tag="RHV13_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HDV1243876.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGTIVV
                     DKRLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGKRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            4693..5547
                     /gene="rclR"
                     /locus_tag="RHV13_004427"
     CDS             4693..5547
                     /gene="rclR"
                     /locus_tag="RHV13_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RclR"
                     /protein_id="HDV1243877.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGMDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
BASE COUNT         1568 a         1231 c         1238 g         1601 t
ORIGIN      
        1 atttattgaa tttcctgaca gtagtctcgt tataaattca tttagacata gcgacagcta
       61 aaccggacga acagggataa atatttcgca aacaacatca ttgctatcct tttctacgaa
      121 atcttcattg taataaaatc gctcaatatc actgccgttc actctggcta accccctggc
      181 gggtaattcc atcagataga ggtttcgcgc ccattcaggg tagtgctccc actcaccttc
      241 atagaggaaa gaggcataca gtcctcggtt aattgtccac tctttcggca tctctgaatc
      301 ggtttgtttg cataccgtac cggcaaaagt ttcaaccgtg agcaaatcga ccgattttgt
      361 tgacggttca aacgtggaga cgaaatagat atccgtctta aaattcttca tacatctggc
      421 taaatgatga cgtcgcagtt tgacttcttc atccggtgat ttattcgaca cttcaatatc
      481 atatttaaat gaattgccaa aaataggtgt ctcaggaaag ttgactaatc tacactccgt
      541 cttttgctgt tgacgtatta aaaaagaagg gaagatattt gcgagatccc aataatcacg
      601 gtggcggtat acacggggag agcagccgaa taacttttta aattcgcggc tgaatgactg
      661 ctgtgaatca aaatgcaaag agagtgcaat atcaagcata gatttcgcgg tgagccggac
      721 aagaatggcg gcacgacaaa gccttcgttt gcgaatgtat tctcccaaag gcacatgcat
      781 gaaatttcgg aacagaagct ggatgttgcg tctgctgtag ccagatttct gtgcgatatc
      841 ttcgattgaa atagggtgct caagattgca ctcaatccac tccaggagct gctgtagaat
      901 cttctgcctg atcatcacat tctccattaa ttcttcgtca tatcgcacat tttcgttatg
      961 ccctgggccc taagtgtatc aaaaacagca aggcccaagg caaaagcact gatagcgtct
     1021 gcttcattgc cccaaaaata gagaggtttc agtcctacat tattaatgaa ttttttgcat
     1081 aagtgatatc aaaatccaca tactaatttg aggttacgtt ttaacgtaga ctcattgttc
     1141 atgcttaatg gagggactga cagtggaatt ttccgtatta agtaacaatc tgaaaatgcc
     1201 gatgatggga tttggtgttt ttcaggttac cgataaaaac gtgtgcaaac agtcagtgtt
     1261 gaacgctatc cgcacgggtt atcgactcat cgacactgca gcggtatacg gtaatgaaga
     1321 tgctgtcggt gaagccgttc gtgaagccat atccgaaggt ttatgtaccc gagaagagtt
     1381 atttataacg tctaaattgt gggtgcagga tatgttgaat caggatacgg cagcagcagg
     1441 tattgaagca tcattaaaaa aatcgggact agaatacttc gacctctatt tattgcatca
     1501 ggctatgcgc gattatttca gtgcgtggcg tgcactcgaa gatgcctatg aagagggcaa
     1561 attaaaagcg attggggttt ccaatttcta tcctcatgtt ctggcgaact tttgtgaaac
     1621 ggtaagagtt aaaccgatgg tcaaccaggt cgagttgcat ccctattttg cccaaccaga
     1681 ggcgctggca accatgaagt attataacgt gcagcctgaa gcatgggctc cgttaggtgg
     1741 tggacgacat aaagcctttg aaaataatct gcttcagagt attgcagatg cccatcaaaa
     1801 atcgatttct caagtcattc tgcgttggaa tattcaacgg ggagtggtcg ttattccgaa
     1861 atcgacacat caacagcgta tcgaagaaaa ttttgctatc tgggatttct cactgacaga
     1921 gaaagaaatg gcacaaatta gttcgcttga tttgggttat gttggggagt cggtaaaaca
     1981 ttttaatcct gaatttgttc gtggttgtct tgctgtaaaa atacataatt gatattaatc
     2041 atgtatttta cctgagacga caagaatctt ttaacagggg agtgatattg atcttcactc
     2101 tgtcatatct ccggtaatat ggcgtcaggc ttcatcatca gaaagggggc ctgacgcctg
     2161 aaaaagtgaa caacagacag tgttcggatt atcaatattc agtttttaac gttgaactgg
     2221 attcattact gcgttgttta agaatttccc gcgccgaatc tgccattatc atgacagcac
     2281 ctgccagcat cagagtatct ttcaatacca agcgaccagc accggataaa taagggaaac
     2341 catgatgagc gtcacccaat gcgggtaccc atgcctccgg ggtggtgatt aaaaatgaga
     2401 gtgttaccaa cggtgtggta aatgccatca gccctcccaa taaacctaac cagcgattga
     2461 caggattagc caaaaccagc agagcaataa tcacctccac gacgccaaga ccgttggaaa
     2521 aaccataggt attattggcc gattgccatg cccttgcttc tggtttgtat tcgccttcgt
     2581 gagtcagata ctgtttatag tcttccgggt gttcataaaa gaacgacatt agtggactgt
     2641 ttgcgacgaa tggtgtaatg ctgtctgcct cgtaagggac aaactttaat aacccaatcc
     2701 acataaaaac aatcgcaata ctcagacgaa tcaatgtcag gccaatttta tctccccgac
     2761 ttaacaggtg caggtatttt tccattgtga acatccttat ttatacaata ctgctgttgc
     2821 ggttgaatta ttatcaattc gcatcgatgt gactttccag gatgaagcat tatattttct
     2881 ggctatatca gcaatggtat cctccagaac tgtcggtgat gctgatactg acgagacgct
     2941 gatatgtttc agaattattt tatcatctgc attggtggaa aatgccataa cgccagataa
     3001 tagtgttgca aataaaacag attttttaaa catataaaat accatcaatg aagtgattaa
     3061 gaagttatag agtaacaggg aattaataag attcttttct ctgagacgcc agaatatttg
     3121 ttctggcgtc tgattttgag tttatttgac taatgaaaat agatcattga gtgattcgct
     3181 catcgacgga tgagtaaata tctgatcgcg taatatgcta taaggcagcc cggcatccat
     3241 caccattttc actatattaa tcatctcgtg ggagtcaaca cacagcagtg atgcccctaa
     3301 tatacgttgg gttttattat caacaatcgc ttttaatacc ccacgggtat cgttcatcac
     3361 tctggcgcgc ggaattgcag ctacaggcaa tgtcaccacc tgaatatcag caccactctc
     3421 tctggcttgt tcttctgtca taccaaccct ggacaggggc ggtgtcataa atacggaata
     3481 aggcacattt ttccgatcat cagtactacg tttgccttca cccagtaact catcacgtac
     3541 aatgcggtaa tcatccagtg atatgtaagt aaattgcagc ccgccggtaa catctcccat
     3601 cgcccaaata ttgtctgcgg tggtatgtaa tcgcttgtca acgacaattg tcccgcgctc
     3661 gtttactgcg ataccggcat tttctggatg taacgaagcg gtagccggtt gacgaccgga
     3721 agctattaac agtgcatcca ccgccagttg ggcgtgctcg ctatgcactt gcacttgatt
     3781 ttcatggtga ctgattcgct ccacatgggc attgaggata atatcgacgc cctgatcgcg
     3841 taaaatcgtc gcgatattat cagcaatatc ccgatcttcc cgaggcaaaa acagcgaagc
     3901 tgcttctaaa atggttacct tgctgccaaa attagcgaac atagaggcga actcaacgcc
     3961 aatatatccg ccgcccaaaa tacctaaatg cccaggcaat tcttttagat taagtaatcc
     4021 ggtgctgtca tatacaccag gcgtggtggt aattccagga attggcggaa ccacggtttg
     4081 tgcaccagta ttaataaaaa ttttctcgcc atgaatctcc agatttccct caggccgatg
     4141 aacacgcagg ctatgattat tgataaactc cgcctggccg tcgatcacgt cgatattggg
     4201 catatctgca agattatgaa aattcttatt acgtaaaaaa ttaaccactt catttttacg
     4261 ctgtatggca cggacaaaat ctgtgtgctg ctgtgcgtca tgaaccaatg ttttggttgg
     4321 gatgcagccg atattaatac aggtcccgcc atacattgca tttgattgtt cgatgagagc
     4381 cacacgccaa cctgcttttg ccagcgtgac ggctaatgtt tttccagcct tgccaaaacc
     4441 aataatcact gcctgatatt tattcatgat gatttcctgt cgaaacgcgt tcatttgaca
     4501 tcatgactat agacgcaaaa gagcaaataa catttcttca caacgcttga tattttgtct
     4561 tatcgtctgg tattttcttt ttcttaatca gaatcacaaa acaaaaatca tgattaacca
     4621 tttgatggta ttagattaac tattgtctaa gattgttggt gcttctaatc aaaaaccact
     4681 caggagtctg atatggatgc ccttagccgt ttgttgatgc ttaacgctcc acaaggaacg
     4741 atcgataaga attgcgtgtt aggaagtgac tggcagcttc cacatggtgc cggggaatta
     4801 tcggttattc gttggcatgc gttaacgcaa ggagcggcga agctggaaat gccgacggga
     4861 gagattttta cattacgccc gggaaatgtg gtcctgctac cacaaaattc agctcatcgc
     4921 ctgagtcatg tcgataatga atcgacctgt attgtctgcg gcactcttcg gttgcaacat
     4981 tcggcgcgtt attttttaac gtctttgccg gaaacgctgt ttttagcacc ggttaaccac
     5041 agcgtggaat ataactggct gagggaggcg atcccgtttt tacaacagga atccagatcg
     5101 gcaatgccgg gaatggatgc tctgtgtagc cagatctgcg ctacattctt taccctcgcg
     5161 gtgcgtgagt ggattgcaca ggttaatact gagaaaaaca ttctcagttt gcttctgcat
     5221 ccacgtcttg gtgcggtaat acagcaaatg ctggaaatgc caggacacgc ctggaccgtc
     5281 gaatcgctgg ccagcatcgc ccacatgtcc cgggcaagtt ttgcccagct tttccgtgat
     5341 gtttccggaa ccacgccgct ggctgtatta acaaagttgc gtctacaaat agcggcccag
     5401 atgttttccc gggaaatgct ccctgttgtg gtgatcgctg agtcagtagg ctatgccagt
     5461 gaatcatctt ttcacaaggc gtttgttcgc gagtttggtt gtaccccggg agaatatcgg
     5521 gaaagggtca gacagcttgc accctgaata aaaccgccag aaatcagggc aaagataatc
     5581 cgcattccgg gagttgtgag cctttcaact atttctattt ccaacggtgg tttgggct
//
LOCUS       DAPGOF010000247         5620 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.250, whole genome shotgun sequence.
ACCESSION   DAPGOF010000247 DAPGOF010000000
VERSION     DAPGOF010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5620
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.250"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            121..1479
                     /gene="mltD"
                     /locus_tag="RHV13_004428"
     CDS             121..1479
                     /gene="mltD"
                     /locus_tag="RHV13_004428"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HDV1243878.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(1527..2297)
                     /locus_tag="RHV13_004429"
     CDS             complement(1527..2297)
                     /locus_tag="RHV13_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDV1243879.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVCLADYP
                     DASVLDMGCGAGHASFVAAQNVSTVVAYDLSAHMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLTLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVDAHKAA"
     gene            complement(2375..3175)
                     /locus_tag="RHV13_004430"
     CDS             complement(2375..3175)
                     /locus_tag="RHV13_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDV1243880.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            3416..4330
                     /locus_tag="RHV13_004431"
     CDS             3416..4330
                     /locus_tag="RHV13_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV1243881.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPNYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDREIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(4327..5130)
                     /gene="dkgB"
                     /locus_tag="RHV13_004432"
     CDS             complement(4327..5130)
                     /gene="dkgB"
                     /locus_tag="RHV13_004432"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HDV1243882.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGLAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKEGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRENLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(5293..5369)
                     /locus_tag="RHV13_004433"
     tRNA            complement(5293..5369)
                     /locus_tag="RHV13_004433"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5333..5335),aa:Asp,seq:gtc)
     gene            complement(5424..5539)
                     /gene="rrf"
                     /locus_tag="RHV13_004434"
     rRNA            complement(5424..5539)
                     /gene="rrf"
                     /locus_tag="RHV13_004434"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1460 a         1364 c         1396 g         1400 t
ORIGIN      
        1 acctgaagag cgttttgcat ggttaaggtc aaagaaagat aggttctgat aaaactttct
       61 tgtcatcggc ttcgttcgcc gttatgatcg gtcgtctttt aagcaactat tgacacacac
      121 atgaaggcaa aagcgatatt actcgcctct gtcctgctcg tgggttgcca gagtaccggc
      181 aacgttcaac agcacgcaca gagcctttct gcagctggtc aaggggaagc agcaaagttt
      241 acaagtcagg cacgatggat ggacgatggg acgtctatcg cgccagatgg tgacttgtgg
      301 gctttcattg gcgacgagct aaagatggga attccggaaa atgaccggat tcgcgaacag
      361 aaacagaaat atttacgcaa taagagctat ctccacgatg taactttacg ggcagagccg
      421 tatatgtact ggatagccgg gcaagttaaa aaacgtaaca tgcctatgga actggtacta
      481 ctacccatag tggagagcgc ttttgatcct cacgcaacgt ctggcgccaa tgccgcaggc
      541 atctggcaga tcattccgag cacggggcgc aattatggtt tgaaacagac ccgcaattat
      601 gacgcgcgtc gcgatgttgt tgcttcaaca actgccgcgc tgaacatgat gcagcgtctg
      661 aacaaaatgt ttgacggcga ctggcttctg accgtagcgg cttataacag cggcgaaggt
      721 cgggtcatga aggcaattaa aacgaacaaa gcgcgtggga aatccacgga cttctggtcg
      781 ttaccgttgc cgcaggaaac gaagcagtac gtgcctaaaa tgctggcatt gagtgatatt
      841 ctcaaaaaca gcaagcgtta tggcgtacgt ctgccaacga ccgatgaaag ccgtgctctg
      901 gcgcgtgtgc acctgagcag cccggttgaa atggcgaagg ttgcagatat ggcggggatt
      961 tccgtcagca agctgaagac attcaacgct ggcgtgaaag gctccacgct gggcgcaagt
     1021 ggtccgcaat acgtgatggt gccaaagaag catgcagatc aactgcgtga atctctggct
     1081 tcaggcgaaa ttgctgctgt acagtcgacg ctggttgccg acaatacgcc gcttaacagc
     1141 cgtgtttaca ccgtacgctc tggcgacacg ctttcaagta tcgcttcacg tctcggcgta
     1201 agcaccaaag atttgcagca gtggaacaaa ctgcgcggat ctaagctgaa gccaggccaa
     1261 agtttgacga ttggtgcagg cagtagcgca cagcgactgg caaacaacag cgatagcatt
     1321 acgtatcgtg tgcgcaaagg cgattcgctt tcaagcattg ctaaacgcca cggcgtgaac
     1381 atcaaagatg tgatgcgctg gaacagcgac actgcgaatc tgcaaccagg cgataagctg
     1441 acgttgtttg tgaaaaacaa caacatgcca gattcctgac aaaccagata ataaaaaggc
     1501 accgattccc ccggtgcctt ttttatttat gccgctttat gtgcatctac catgatgata
     1561 tcactggtga aagagccatc attctgcaag gcaaaatacg ttctcacctc tgtcgatgcg
     1621 ctctgttggt aaatgcgaat agcgtctact aacgcttctg gcgtacgcat tctcgcgacc
     1681 catgaagaaa attccagcgg taacttatct gtaattaaat tatcaactat cagattggct
     1741 tcattgatta acgtcaacca ctcaccgctg gcgtagtttc gtacgtgaga ggtatcgcgt
     1801 aatgcttcta ccgtctgtaa ccagatgtcg cgcactgggt gacccggaga cattacgtcc
     1861 atcacaatca gcctaccgcc aggtttcaat atccggttca cttctcgcag tgctgcacca
     1921 acatcatgcc agtgatgggc agaataacgg ctgataacaa tatcaaatgc gttatcggca
     1981 aatggcagac tttcggcata tccctggcgg gtggtgatat ttttcagttg ccgggcttcg
     2041 gcagcttgtg ccacgacatc cagcatatgg gcagataagt catacgccac caccgtgctc
     2101 acgttttgcg cagcgacaaa gctggcatgc cctgctccgc agcccatatc aagtacactt
     2161 gcatcaggat aatcagccag acacactgcc aggcgctgta aatctcgtcc ggatgcatgt
     2221 acggtactgg tcagatattc gctggcttga gagctaaact gcttttctac gtggtcatgg
     2281 tgggattgtg ttgtcattac gctgtccttt tgttgtttga gtataaaagg gcagcgccat
     2341 acgttggtct gccctgtggc agacctgaca taccttattt atcaggcttg ccgggactga
     2401 attcaacgag tagcggattg tggtcagaag cgcgcgtaac cagtacagaa gcttcgctga
     2461 cgttcagacc acggtagaaa acaaaatcga gcgggcgacc aaacgcccga cggcgctgat
     2521 catcggtaaa acgcacctgg cgcagcgaca tttcccgcgc aaagcgatat aacgcgttca
     2581 tccttctacg gctccaggca ttgaaatctc ctgccataat gaccgggccg ctgtggtgag
     2641 ctatctgatc accaatagga agtaactgct tactatagac atccacgccc agactgaagt
     2701 tgacggcgtg tatattaacc accatcaaca ggcgggtgtc aggtaatgga tagaccgtca
     2761 ccagtgccga cttcgccaga cgcaaaatgg gttctcgttc acgtaacggg cagcaataca
     2821 ctggatgcgc cgccgaaagg gtcattacgc cagaaggatg ttgtggcagc acgaaagcgg
     2881 gtacctgatc ggcggcaaga tagttagcgg tcgcaaactg tactaactct ggcgttgtct
     2941 gcgcttcctg caataacacc agatgtgcat ctttgccgta gttctttaat accgacaacc
     3001 attcagcgcg ttgctgtttg tatatgttcc acaccaggat ccgaatacgc tcttcggtac
     3061 ttaacggttc tccaggtggt aatgcctggc cgatgctcgc aaaagacccc ggcggtaaga
     3121 tcctttccgc aggttgtccg gcaacatagc gcatggcata ggtgtttttt cgcacttttg
     3181 actttgaaac ctctattacg tcaacccttc cgcactcggg agggttctac tgttataggg
     3241 attttagctc actctttcaa cgagcaattg tatattgtta tgtaagcaag tgcttacacc
     3301 ggtcagaatt ttgctcatta ttgagaacat taaatcggat gacatatccc gcatcctgat
     3361 ggtttaatga caaataaatt caaaatatct cttctatttt gcaacaggag caaaaatgaa
     3421 agccacatcg gaagaactcg ccatttttgt ttcggtcgta gaaagcggca gctttagccg
     3481 ggcagcggaa caattagggc aagcaaactc tgcggtaagc cgggcggtga aaaagctgga
     3541 gatgaaactt ggcgttagcc tgcttaatcg gaccacgcga caacttagcc tgacggaaga
     3601 aggcgagcgt tattttcgtc gcgtacagtc aattttgcag gagatggcag cggcagaatc
     3661 agaaattatg gagacgcgta atacaccgcg tggactgtta cggatcgatg ccgcaactcc
     3721 agtggtgctg cactttctga tgccgttaat taagcctttc cgtgaacgct atccggaagt
     3781 cactttgtcg ctagtctcct ctgaaacgat tattaatttg atcgaaagaa aagtggatgt
     3841 cgcgatacgc gctggtacgt taacggattc cagcttacgt gccaggccgt tatttaacag
     3901 ttatcgaaaa attatcgcct cccccaatta tatttcccgc tacgggaagc cagaaacgat
     3961 cgacgattta aagcaacatg tttgcctggg attcactgaa cccgcttccc tcaatacctg
     4021 gccgatagcc tgtagcgatg gacaattaca tgaggtgaag tacggtttgt catccaatag
     4081 tggggaaaca ctgaaacagc tttgcctgag tggcaacggg attgcgtgtt tgtccgacta
     4141 tatgatcgac agagaaatcg ctcgcggaga attggtggag ttaatggcag ataaagtgtt
     4201 gccagtggaa atgccattca gtgcagtcta ttacagcgac cgtgcggtaa gtacgcgcat
     4261 ccgggctttt atcgatttcc ttagtgagca tgtaaaaaca gctcccggag gagctgtcag
     4321 agaggcttaa tcccattcag gagccagacc ttccgggcta accaggcggt cgttgcaatc
     4381 cagtgcggcg atcgcttttt tatcttcggc atcaagctgt aaattttgtg ccttaagatt
     4441 actctccagg ttttcacgtt tagtagaaga aggaattact gagtaacctt cccccatagc
     4501 ccacgccaga atcacttgtg ccggagtcgc attgtgttta gctgcgatac gagcaataac
     4561 ctcatctttc agagccttac catacgccag cgtcatatag gaagtaatat ggatgccgtg
     4621 ctgtttagcc caggcaacca ctttacggtt ttgcagataa ggagagagtt caatctggtt
     4681 agtagcgatg ttttcagcgc caacagcagc aatcgccttt tccatcaatg ggatcgtgaa
     4741 gttggaaata ccgatctcac gcgtcaaccc ttcttttttg gcttccagca gcgcctgcat
     4801 aaactcttca acagagactt catcgtttgg tgacggccag tggattagag tcagatcaac
     4861 ataatcggta cgcaattttt gcaggctctc ttttagactc gggatcaatt tgtctttgct
     4921 gagattttca atccagattt tagtggtgat gtagagttca tgacgtggca cgccactttc
     4981 tgcaatcgcc agacctactg cggcttcgtt atcatagatt tgtgcagtat caattgcgcg
     5041 ataaccaagt tcaagcgccg ttttcacaga tgaaataaca acgtcgtctt tcagacggaa
     5101 agtacctaaa ccaaatgcag ggatagccat aatatgcctc ttttaatgct attttcgtta
     5161 actgaggtga ttatgggctt tagaatgttg cggaaaaagg gcgtaaaacg cagaggattt
     5221 ttgcgatttt agaaataata gaaatgtaga cgaaaaaaaa cctcgagcga taactcgagg
     5281 cttcttaata agtggcggaa cggacgggac tcgaacccgc gaccccctgc gtgacaggca
     5341 ggtattctaa ccgactgaac taccgctcca ccgaattctt ttacaaccac cggttttatg
     5401 accggcttac tgctttattt gatgcctggc agttccctac tctcgcatgg ggagacccca
     5461 cactaccatc ggcgctacgg cgtttcactt ctgagttcgg catggggtca ggtgggacca
     5521 ccgcgctacg gccgccaggc aaattctgtt atatcagacc gcttctgcgt tctgatttaa
     5581 tctgtatcag gctgaaaatc ttctctcatc cgccaaaaca
//
LOCUS       DAPGOF010000248         5549 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.251, whole genome shotgun sequence.
ACCESSION   DAPGOF010000248 DAPGOF010000000
VERSION     DAPGOF010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.251"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            69..410
                     /locus_tag="RHV13_004435"
     CDS             69..410
                     /locus_tag="RHV13_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243883.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            456..893
                     /locus_tag="RHV13_004436"
     CDS             456..893
                     /locus_tag="RHV13_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HDV1243884.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(935..1930)
                     /gene="wecH"
                     /locus_tag="RHV13_004437"
     CDS             complement(935..1930)
                     /gene="wecH"
                     /locus_tag="RHV13_004437"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HDV1243885.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLFFYSAIALLYIALFTSINV
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALVTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            2105..2404
                     /locus_tag="RHV13_004438"
     CDS             2105..2404
                     /locus_tag="RHV13_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HDV1243886.1"
                     /translation="MMMNSFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            2499..3410
                     /gene="glyQ"
                     /locus_tag="RHV13_004439"
     CDS             2499..3410
                     /gene="glyQ"
                     /locus_tag="RHV13_004439"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDV1243887.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            3420..5489
                     /gene="glyS"
                     /locus_tag="RHV13_004440"
     CDS             3420..5489
                     /gene="glyS"
                     /locus_tag="RHV13_004440"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDV1243888.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPSEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
BASE COUNT         1410 a         1359 c         1430 g         1350 t
ORIGIN      
        1 atggcatttt tcagttttgt ctttcgttgg ttactcgtaa tgtatcgctg gtagatatgg
       61 agatcgttat gaaaacctca aaaactgtgg caaaactatt atttgttgtc ggggcgctgg
      121 tttatctggt tgggctatgg atctcatgcc cattgttgag tggaaaaggc tattttcttg
      181 gcgtgttaat gacagcaact tttggcaact atgcatatct tcgcgcagaa aaactcgggc
      241 aactggataa tttttttacc catatttgcc agttagttgc gttaatcact atcggtctct
      301 tgtttatcgg tgttttaaat gcacctatca atgcttatga aatggtgatc taccccatcg
      361 ccttttttgt ctgcttgttt ggtcaaatgc gtttgtttcg ctcggtatga gcaacataaa
      421 gctcttacac attcaggaat gaaaggaata ctgtgatgga caacaaaata tcaacctatt
      481 caccggcctt tagtattgtg tcatggatag ctctcgttgg tggtatcgtt acctatctgt
      541 tagggctatg gaatgcagag atgcagttaa atgaaaaagg atattatttt gccgtactgg
      601 tattaggact gttttctgcg gcgtcttatc aaaagaccgt tcgggacaag tatgaaggca
      661 taccgaccac ttccatttat tatatgacct gcctgactgt ctttattatc tctgttgcat
      721 tactgatggt aggtctgtgg aatgcgacat tattactcag cgaaaaaggt ttttatggac
      781 tggctttctt cttaagcttg tttggtgcag tagcggtgca gaaaaatatt cgtgatgccg
      841 gaataaaccc accaaaagaa acacaggtta cccaggaaga atacagcgaa taactcacgt
      901 aagcccggtc agtccaatgt gaccgggctt ttacttaact cactaatctg tttctgtcga
      961 ttcgttgtac cagcatagaa agtaacaaac tcgctgccaa cgtcgcgcaa aaaatccaaa
     1021 taatatccag tattggccaa tttttaagct caattccccg ggtgcgcagc gcatggataa
     1081 tcaaggcgtg gaatccgtat atacccaacg aatggcggga gattaagcca agtccgcgaa
     1141 tggtacgcgt atccagcgtg tttttaacca gagtcactag cgcgattgcg cagataaaaa
     1201 ccatcggccc acagtaaaga taccaggtat cggcaaaatt tccgcgccac tgcaattcat
     1261 ataatgtccc gcgagagata ataaaaactc ccgtcgcaaa cagtgcggcg ctcacccacg
     1321 acagtgcttt atgctgtgtg tccatcatcc ctatagcgcg gcccaacatg ccatacagaa
     1381 tgtagtaaaa agtatcgcca ttgatatata agttaattgg cagccattca aaaccgtcga
     1441 ttttctgcgg cacggtgttt gggttagcga tgataccaat caccaccatt agcaccagca
     1501 acatttttcc gccgacgttc ttcacctgaa tcagcggtga aaccagataa atcaccgcaa
     1561 tcgcgaagaa aaaccataag tggtaaaaca ctggcttttg cagcaggttt ttcagcgcta
     1621 actccacatt aatggaggta aacagtgcaa tgtagagcag cgcgattgcg ctataaaaaa
     1681 acagacataa gccgatacgc aagaaatggc gcggctgggc gctgcgttcg ccaaaaaaga
     1741 gatagccgga aatcatgaaa aatagcggca cgctgacacg agaggcagag ttcagaacat
     1801 tggcgatatc ccaggtgacg gggctaacac tatgagcatt ggtcacatac caggtagtgg
     1861 tgtgaatcat caccaccatt aaacacgcta tccctcgcag gttatcaatc cagtaaattt
     1921 tgggctgcat ctgtgtctct gtatctggtt aaaaaaagtc tgaccgataa atcattcagt
     1981 tggcgcactg gaataatctg agttttaaca ctacagctta tagaggctta aggaaattcg
     2041 taagatatca gccactatac cgatataaat aataagactc acctgcaaac cagacggtaa
     2101 tttaatgatg atgaactctt tctttccggc aatggcgctt atggtgctag tgggttgttc
     2161 tacaccgtca cccgtgcaga aagcacaacg ggtaaaggtt gatcctctgc gttcgttgaa
     2221 tatggaagcg ttatgcaagg atcaggcggc aaaacgttat aacaccggcg agcaaaaaat
     2281 cgacgtcacc gccttcgaac agttccaggg aagctatgaa atgcgcggtt ataccttccg
     2341 taaagagcag tttgtctgtt cttttgacgc agatggccat tttttgcatc tttccatgcg
     2401 ttaagccctg ctttttcccg tttcgtactg tatatcttcc atccagcggg tatactgatc
     2461 ccttccttta aatccacacg tatccagcac gaaataatat gcaaaagttt gataccagga
     2521 ccttccaggg cttgatcctg accttacagg attactgggc tcgccagggc tgcaccattg
     2581 ttcaaccatt ggacatggaa gtcggcgcgg gaacctctca cccaatgacc tgtctgcgcg
     2641 cgctggggcc agaaccgatg gcggctgctt atgttcagcc ttctcgtcgc ccgaccgatg
     2701 gtcgctacgg cgaaaacccc aaccgtttac agcactacta tcagttccag gtggtcatta
     2761 agccatcgcc ggacaatatt caggagctgt accttggttc tctgaaagag ctgggcatgg
     2821 acccgactat ccacgatatc cgtttcgtgg aagataactg ggaaaacccg acgctgggtg
     2881 cctggggact gggctgggaa gtgtggctaa acggcatgga agtgacgcag ttcacttact
     2941 tccagcaggt tggtggtctg gagtgtaaac cggttaccgg cgagatcacc tacggtctgg
     3001 aacgtctggc catgtacatt cagggcgtag acagcgttta cgacctggtc tggagcgacg
     3061 gcccgctggg taaaaccacc tacggcgacg tgttccatca gaacgaagtg gagcagtcca
     3121 cttacaactt cgaatacgcg gacgtggact tcctgttcac ctgcttcgag cagtacgaga
     3181 aagaagcgca gcagttgctg gcgctggaaa atccgctgcc gctgccagcc tacgagcgta
     3241 ttctgaaagc cgcccacagc ttcaacctgc tggatgcgcg taaagccatc tccgtcactg
     3301 agcgtcagcg ctacattctg cgcattcgca ccctgaccaa agcagtggca gaagcatact
     3361 acgcttcccg tgaagccctc ggcttcccga tgtgcaacaa agataagtaa gaggcggcta
     3421 tgtctgagaa aacttttctg gtggaaatcg gcactgaaga gctgccacca aaagcactgc
     3481 gcagcctggc agagtccttt gctgcgaact ttactgcgga gctggataac gcaggcctcg
     3541 cacacggcac cgttcaatgg tttgctgctc cgcgtcgtct ggcgctgaaa gtagctaacc
     3601 tggcggaagc gcaaccggat cgtgaaatcg aaaaacgcgg cccggcgatt gcccaggcgt
     3661 tcgacgctga aggcaaaccg agcaaagcgg cagaaggttg ggcgcgtggt tgcggtatta
     3721 ccgttgacca ggctgagcgt ctgactaccg ataaaggcga atggctgctg tatcgcgccc
     3781 atgtgaaggg cgaaagcact gaagcactgc tgccgaatat ggttgcgact tcgctggcga
     3841 aactgccgat tccgaaactg atgcgctggg gtgcaagcga cgtgcacttc gtgcgtccgg
     3901 tgcacaccgt gaccctgctg ctgggcgaca aagtcattcc ggcaaccatt ctgggcattc
     3961 agtccgatcg cgtgattcgc ggccaccgct ttatgggcga gccggaattc accatcgata
     4021 acgccgatca gtatccggaa attctgcgtg agcgtgggaa agtcatcgcc gattacgaag
     4081 aacgtaaggc gaagattaaa gccgatgccg aagaagcagc gcgtaagatt ggcggtaacg
     4141 ctgacttaag cgaaagcctg ctggaagaag tggcttcgct ggtggagtgg ccggtcgttc
     4201 taaccgcgaa atttgaagag aaattcctcg cggtgccgtc tgaagcactg gtttacacca
     4261 tgaaaggtga ccagaagtac ttcccggtgt atgcgaacga cggcaaactg ctgccgaact
     4321 ttatcttcgt tgccaacatc gaatcgaaag atccgcagca aattatctct ggtaacgaga
     4381 aagtcgttcg tccacgtctg gctgatgccg agttcttctt caacaccgac cgtaaaaaac
     4441 gtctggaaga taacctgccg cgcctgcaaa ccgtgctgtt ccagcaacag ctgggtacgc
     4501 tgcgtgacaa gactgaccgc atccaggcgc tggctggctg gattgctgaa cagattggcg
     4561 ctgacgttaa ccacgcaacc cgtgcgggcc tgctgtctaa gtgtgacctg atgaccaaca
     4621 tggtcttcga gttcaccgac acccagggcg ttatggggat gcactacgcg cgtcacgatg
     4681 gcgaagcgga agacgtagcc gtcgcgctga atgagcagta tcagccgcgc tttgccggtg
     4741 atgacctgcc gtccaacccg gtagcttgtg cgctggcgat tgctgataag atggatactc
     4801 tggcgggtat cttcggtatc ggtcagcatc cgaaaggcga caaagacccg tttgcgctgc
     4861 gtcgtgccgc acttggcgtg ctgcgaatta tcgttgagaa gaacctcaac cttgatctgc
     4921 aaacgctaac cgaagaagcg gtgcgtctgt atggcgataa gctgactaat gccaacgtag
     4981 ttgatgatgt tatcgacttt atgctcggtc gcttccgcgc ctggtatcag gacgaaggtt
     5041 ataccgttga caccatccag gcggtactgg cgcgtcgtcc gactcgtccg gctgatttcg
     5101 atgcccgcat gaaagcggta tcgcacttcc gtacccttga agcagctgct gcactggcgg
     5161 cggcgaacaa gcgtgtctcc aacattttgg cgaaatctga cgaagtgctg agcgatcgcg
     5221 tgaatgcctc tactctgaaa gagccggaag aaattaaact ggcgatgcag gttgtggtgc
     5281 tacgtgacaa gctggagccg tactttgcgg aaggtcgtta ccaggatgcg ctggtcgaac
     5341 tggcagagct gcgtgaaccg gtcgatgcct tcttcgataa agtgatggtc atggttgatg
     5401 acaaagaatt gcgtatcaac cgtctgacca tgctggagaa actgcgcgaa ctgttcctgc
     5461 gcgttgcgga tatttcgctg ttgcagtaat aacgccggta ttaaaaagcc tgccatctgg
     5521 caggcttttt ttatcgctaa ataatacag
//
LOCUS       DAPGOF010000249         5372 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.252, whole genome shotgun sequence.
ACCESSION   DAPGOF010000249 DAPGOF010000000
VERSION     DAPGOF010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.252"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..1148)
                     /gene="tuf"
                     /locus_tag="RHV13_004441"
     CDS             complement(<1..1148)
                     /gene="tuf"
                     /locus_tag="RHV13_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDV1243889.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDV
                     FSISGRGTVVTGRVERGIIKVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVG
                     VLLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFR
                     TTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDDGLRFAIREGGRT"
     gene            complement(1263..1338)
                     /locus_tag="RHV13_004442"
     tRNA            complement(1263..1338)
                     /locus_tag="RHV13_004442"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1303..1305),aa:Thr,seq:ggt)
     gene            complement(1345..1419)
                     /locus_tag="RHV13_004443"
     tRNA            complement(1345..1419)
                     /locus_tag="RHV13_004443"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1384..1386),aa:Gly,seq:tcc)
     gene            complement(1536..1620)
                     /locus_tag="RHV13_004444"
     tRNA            complement(1536..1620)
                     /locus_tag="RHV13_004444"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1584..1586),aa:Tyr,seq:gta)
     gene            complement(1629..1704)
                     /locus_tag="RHV13_004445"
     tRNA            complement(1629..1704)
                     /locus_tag="RHV13_004445"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1669..1671),aa:Thr,seq:tgt)
     gene            2066..3016
                     /gene="coaA"
                     /locus_tag="RHV13_004446"
                     /gene_synonym="panK"
     CDS             2066..3016
                     /gene="coaA"
                     /locus_tag="RHV13_004446"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDV1243890.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(3045..4010)
                     /gene="birA"
                     /locus_tag="RHV13_004447"
     CDS             complement(3045..4010)
                     /gene="birA"
                     /locus_tag="RHV13_004447"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HDV1243891.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(4007..5035)
                     /gene="murB"
                     /locus_tag="RHV13_004448"
     CDS             complement(4007..5035)
                     /gene="murB"
                     /locus_tag="RHV13_004448"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDV1243892.1"
                     /translation="MNHSLKSWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
     gene            complement(5170..5285)
                     /gene="rrf"
                     /locus_tag="RHV13_004449"
     rRNA            complement(5170..5285)
                     /gene="rrf"
                     /locus_tag="RHV13_004449"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1341 a         1382 c         1235 g         1414 t
ORIGIN      
        1 gtacggccgc cttcacggat tgcgaaacgc agaccgtcgt ccatcgcgat cgggtggatc
       61 agggtaacaa ccattttgat gttgtcgccc ggcattacca tctctacgcc ttccggcagt
      121 tcgatggtac cagtcacgtc agtagtacgg aagtagaact gcggacggta gcctttgaag
      181 aacggagtat gacggccgcc ttcatctttg gacagaatgt acacttcaga ttcgaacttg
      241 gtgtgcggct tgatggtgcc cggcttagcc agtacctgac cacgttcgat ttcttcacgt
      301 ttgataccac gcagcagaac acctacgttc tcaccagcac ggccttcgtc cagcagtttg
      361 cggaacattt caacgccagt acaggtagac ttctgagtct ctttgatacc aacgatttca
      421 acttcttcac caactttgat gataccgcgt tctacacgac cggtaacaac ggtaccacga
      481 ccggagatgg agaatacgtc ttcgatcggc agcaggaacg gcttgtcaat cgcacgctct
      541 ggttccggaa tgtaagaatc caggaagcca gccagttcca ggattttcgc ttcccactct
      601 gcgtcgcctt ccagcgcttt cagagcagaa ccacgaacga tcggagtgtc gtcgcccggg
      661 aagtcgtact gagacagaag ttcacgaact tccatttcaa ccagttccag cagctcttcg
      721 tcatcaacca tgtcgcattt gttcaggaac acgatgatgt acggaacgcc tacctgacga
      781 cccagcagga tgtgctcacg agtctgcggc atcgggccgt cagtcgcagc aactaccagg
      841 atcgcgccgt ccatctgagc agcaccggtg atcatgtttt taacatagtc ggcgtgcccc
      901 gggcagtcta cgtgtgcgta gtgacgggtc ggggtgtcgt attcaacgtg agaagtgttg
      961 atggtgatac cacgagcttt ttcttccggc gcgttatcga tctggtcgaa tgcgcgagca
     1021 gcaccgccgt aggttttagc cagtacggta gtgattgcag cggtcagcgt tgttttacca
     1081 tggtcaacgt ggccgatagt accgacgtta acgtgcggtt ttgtacgttc aaacttttct
     1141 ttagacatcg attgtccctc taagacacgg ataaatcggt gatatcacca catcaaccag
     1201 gcaacatgcc cgacttgttg aatgcaataa acagaaggaa aaaacaggga ggagaaaaga
     1261 agtggtgctg ataggcagat tcgaactgcc gacctcaccc ttaccaaggg tgcgctctac
     1321 caactgagct atatcagcac atcttggagc gggcagcggg aatcgaaccc gcatcatcag
     1381 cttggaaggc tgaggtaata gccattatac gatgcccgca tcctggaact cggctacctg
     1441 attttcattc tgcactgaat atcgagagaa gctctcttta ttcgagccgg taagcgaact
     1501 tatcgtctcg ggctacgcca tcgcgtggcc gaaattggtg gtgggggaag gattcgaacc
     1561 ttcgaagtct gtgacggcag atttacagtc tgctcccttt ggccgctcgg gaaccccacc
     1621 ggacttgatg gtgccgacta ccggaatcga actggtgacc tactgattac aagtcagttg
     1681 ctctacctac tgagctaagt cggcatcaag tagcgcgcat tctatggaga catgcgagtt
     1741 catgcaacta aaaaattgca taatttgttt tattggtcac attttatgcg acacgatgaa
     1801 gaaacagccg aacggagggc agattagcac actttttcaa catcattgtg ctcaacaatg
     1861 cgctcctgct aaaccataat tctttttatc agatggaata tctgtcacat tgcttttcaa
     1921 cgatagcttc ctggcagaga ttttttctta ttattcctcc ccatctggtg ttaccctcct
     1981 gcccattaac ccattcaaca gaactgtgac gcgccatggc aaatatcgct ttgccgatag
     2041 agctatgacc gccagaaaca tgcttatgag tataaaagag caaacgttaa tgacgcctta
     2101 cctacagttt gaccgcaacc agtgggcagc tctgcgtgat tccgtaccta tgacgttatc
     2161 ggaagatgag atcgcccgtc tcaaaggtat taatgaagat ctctcgttag aagaagttgc
     2221 cgagatctat ttacctttgt cacgtttgct gaacttctat ataagctcga atctgcgccg
     2281 tcaggcagtt ctggaacagt ttcttggtac taacgggcaa cgcattcctt acattatcag
     2341 tattgctggc agtgtcgcgg tggggaaaag tacaaccgcc cgtgtattgc aggcgctatt
     2401 aagccgttgg ccggaacatc gtcgtgttga actgatcact acagatggct tccttcaccc
     2461 taatcaggtt ctgaaagaac gtggtctgat gaagaagaaa ggcttcccgg aatcgtatga
     2521 tatgcatcgc ctggtgaagt ttgtttccga tctcaaatcc ggcgtgccaa acgttacagc
     2581 ccctgtttac tcgcatctta tttatgatgt gatcccggat ggagataaaa cggttgttca
     2641 gcctgatatt ttaattcttg aagggttaaa tgtcttacag agcgggatgg attatccaca
     2701 cgatccacat catgtatttg tttctgattt tgtcgatttt tcgatatatg ttgatgcacc
     2761 ggaagactta cttcagacgt ggtatatcaa ccgttttctg aaattccgcg aaggggcttt
     2821 taccgacccg gattcctatt ttcataacta cgcgaaatta actaaagaag aagcgattaa
     2881 gactgccatg acattgtgga aagagatcaa ctggctgaac ttaaagcaaa atattctacc
     2941 tactcgtgag cgcgccagtt taatcctgac gaaaagtgct aatcatgcgg tagaagaggt
     3001 cagactacgc aaataatttg caggggagcg aatactcccc tttcttattt ttctgcacta
     3061 cgcagggata tttcaccgcc catccagggt tttattattc catcctgctc aagtaataaa
     3121 gccccctgtt tgtctattcc gcgtgaaatg ccaaatattt ctttatcacc aatgataagt
     3181 ttcactgggc gattaataaa attatccagc ttttcccagc gcgacagata aggtgccaat
     3241 ccttcttgtt cgaagagttc caacgcagca cgtaattcac gtattagcat ggccgccaac
     3301 gtattacgat cgagattgat ccccgcttcc tgcagcgtga tccacccctg attaacgaca
     3361 ctctcttcaa cacggcgcat tgccatgttg atcccggctc caatgactat ttgcgccgca
     3421 tcgccagttt tgccagtcag ctccacaaga atgcctgcca gcttgcgatc ctgcagatag
     3481 aggtcattag gccatttgac acgaacttta tctgctccca gcttgcgtaa tacttccgcc
     3541 atcacgatac cgataaccag acttaaacca atcgccgccg ccgggccttg ttccagacgc
     3601 cagaacatcg acaaatataa gtttgcgcca aaaggcgaaa accatttccg cccccggcga
     3661 ccacggccag cctgctggta ttctgcaaca caggcatcgc ccgatttaag ctctccgata
     3721 cgatcaagaa ggtactgatt cgtggagtca ataactggca gcacggctac actaccgcca
     3781 tccagctgac ccaatatctg ttcagcatta agtaactgga tgggctcagg caggctgtat
     3841 cctttacccg gaacggtaaa gacatcaacg ccccagtcac gcagtgtctg aatgtgttta
     3901 ttaatagccg cccggctcat tcccagcgtt tcacccaact gctcgccaga gtgaaattca
     3961 ccgttcgcta acagggcaat caatttcagt ggcacggtgt tatccttcat gaaattgtct
     4021 ccactgcgct cacttcacct gatgcaccaa taaagcggac ttcaggctca agccagacat
     4081 taaatttttc acccactttt tgtcttacat ggtgtgccag ttgcaccaca tcttcgcttt
     4141 ttgcattgtc ttcattaatg agaactaacg cctgttgacg gtgcaccgca gccccaccca
     4201 tttgcatccc ttttagctgg cactgatcaa taagccaacc tgctgccagt tttactgaac
     4261 cacccgcctg ggggtaattt ggtgctgttg gaaattgcgc cagtaatgct ttagccgttt
     4321 cggcagatac aacagggttt ttgaagaaac taccggcatt gccattcact tttggatcag
     4381 ggagtttggt ggtgcgcata tgacacaccg cattaaatac ttgctgtggc gttactgttg
     4441 taggatccag acgagttaag tcaccatacg ttagtacagg ttgccactct tttggcagac
     4501 gcagacctac ggctacaatg gcgaagcgat cctggtattc atgtttaaaa atactgtcgc
     4561 gatagccaaa acggcactct ttggcagtta agcgcacttg cttgcctgtc gccagttcaa
     4621 cacaatcaac ataagcgcaa actcgctgta attctacgcc ataagcacca atattctgga
     4681 taggtgatga gccgacacaa ccaggaatta atgccagatt ttccagacca ggcatacctt
     4741 cctgcaaagt gtattttacc aggcgatgcc agttttctcc ggctcctaca tgtaaatacc
     4801 acgcatcagg ttcatcatga atttcgatac ctttgatccg gttgatgatc accgtgccgc
     4861 gatagtcttc cagaaaaagt acattacttc cttctcccag aataagaacg ggttgccctt
     4921 ctgcggttgc atgctgccag gcattgagta gttgttgttc gtcttcggca catacaatgt
     4981 gctgagcatt atgatcaatg ccaaatgtgt tccaggattt taaggagtgg ttcatagctg
     5041 ctttcctgat gcaaaaacga ggctagttta ccgtatctgt ggggggatgg cttgtagtta
     5101 tgacgacagg gagagtttgt agaaacgcaa aaaggccatc ctgacggatg gcctttcgct
     5161 ttatttgatg cctggcagtt ccctactctc gcatggggag accccacact accatcggcg
     5221 ctacggcgtt tcacttctga gttcggcatg gggtcaggtg ggaccaccgc gctaaggccg
     5281 ccaggcaaat tctgttttat taacccgctt accgccggtt aatctaatct aatctgtatc
     5341 aggctgaaaa tcttctctca tccgccaaaa ca
//
LOCUS       DAPGOF010000250         5303 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.253, whole genome shotgun sequence.
ACCESSION   DAPGOF010000250 DAPGOF010000000
VERSION     DAPGOF010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.253"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1414..2568
                     /locus_tag="RHV13_004450"
     CDS             1414..2568
                     /locus_tag="RHV13_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDV1243893.1"
                     /translation="MPSPRIRKMSLSRALDKYLKTVSVHKKGHQQEFYRSNVIKRYPI
                     ALRNMDEITTVDIATYRDVRLAEINPRTGKPITGNTVRLELALLSSLFNIARVEWGTC
                     RTNPVELVRKPKVSSGRDRRLTSSEERRLSRYFREKNLMLYVIFHLALETAMRQGEIL
                     ALRWEHIDLRHGVAHLPETKNGHSRDVPLSRRARNFLQMMPVNLHGNVFDYTASGFKN
                     AWRIATQRLRIEDLHFHDLRHEAISRFFELGSLNVMEIAAISGHRSMNMLKRYTHLRA
                     WQLVSKLDARRRQTQKVAAWFVPYPAHITTIDEENGQKAHRIEIGDFDNLHVTATTKE
                     EAVHRASEVLLRTLAIAAQKGERVPSPGALPVNDPDYIMICPLNPGSTPL"
     gene            2719..3543
                     /locus_tag="RHV13_004451"
     CDS             2719..3543
                     /locus_tag="RHV13_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HDV1243894.1"
                     /translation="MRLNTTGIAARMMLSLDKKAIGEKLINGRQINPTPLQVRYPQGV
                     REVLGIMSEQLAISTADLTRILLEDALHNMFMPADNTTGNIISRIEYIMLSHDINAPL
                     LATLLSPWNIRSTVIQDPARLADYLSADALEHLAECFNLNPDWLNGHENYPIALSGEW
                     PDTADNFRMLISDSSNTEVIFWHSFPFAGNTKREYCGVILRQKKEINGSVIYPALSLS
                     PTLLNDEKRKWLTEYTTRQNTTMSLRRVTLRPGLAGNLITGQILPVSLFNTSLLPW"
     gene            3628..4830
                     /locus_tag="RHV13_004452"
     CDS             3628..4830
                     /locus_tag="RHV13_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HDV1243895.1"
                     /translation="MMKKNHITRTIIASAVLFSFNAAAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKHNSNDDFSLLLPSVGAQVADPDDVSNKADDVKDDWDLFDSAVD
                     NQHGVQQAAANLKHRLQEFRNINADAQVGVSAVAAMANDTLPFALMVKSYGTVSVNGK
                     VNDADLDYLDKVANGTITDVDKNALTSRAFGRAAVITDVGISFAKELETAGQKWSLGV
                     TPKYQRVDLFNYNVTVRDYDKDDFDGDKYHNTKNGFNADIGAYTDLNDNWTVGLVAQN
                     IIPRSIDTKVVNGFKETFKVRPQATAGVSWHNDLFTTALDIDLTPASGFTSDSKRQFA
                     SVGAEFNAWKWAQLRAGYRQNMASNSGSAFTAGVGISPFDVVHIDVSGLVGTDHDYGA
                     MAQLQFTF"
     gene            4890..>5303
                     /locus_tag="RHV13_004453"
     CDS             4890..>5303
                     /locus_tag="RHV13_004453"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase"
                     /protein_id="HDV1243896.1"
                     /translation="MMKPRSSYSKTAFILLFSVFLVAAVTKAKSSLPDITLEQAKEIN
                     ADNTVIFLFRHGERCDRSDMPCYSDKSGITITGTEKAQQEGIKFATIFSEYDIYSSNA
                     VRTIQTAKFFSGKEPVVMDSLSDCNNDLYKTLESIA"
BASE COUNT         1508 a         1258 c         1144 g         1393 t
ORIGIN      
        1 ggcctacgtg ccaatccggt acctggggcg ctcctaaaat tcaattcaca acgcagacct
       61 acaacatagc taaaaacact cgtaatttaa ggctcggggt ccatgcatat tgctcatgga
      121 cttacctgaa tggctcaccc tttggtggtt ttcaacaggt atattccgac caaaacaacg
      181 tttggtatgt gagtaattat gcttggggaa attatgagtc tggtgggacc atatcagtca
      241 catgcctcaa tcttcctggt gctggagctt aattaaaggg gcaacagtag gcatcgacat
      301 gttcctcatc aacaccatca ccaataccgt acatgcgtgt tccagtcata atgtatccag
      361 tcggacattg aatcggcttg taatagatcg aaccaccact cttaccgccg ataaagtgat
      421 tcattgctac ggatgatttg taccaattac atgctgagta attaatttta ttaccccctg
      481 accaccgacc ggattggcac gtaggccagc agcgccagca atttaatctc agttctgaca
      541 cgcgacgaca taactacatt ttccggttaa atcagctcta aatgtccctt tatggaaatt
      601 gtattcatca taaaaaccac atttaacaat tcgatttgca tcataagttg caactgatcc
      661 tgcaaagtaa ttcggcttcc aaccttgagg gttttctgca ttgctcacac tccatgagca
      721 ctgactggca gaaaatcctg ttggcagcgg tatttgctga ccatttgcta tacgaccagt
      781 tatcaccgta ctaccagaat tgcttttacg ccaagtaccg gattggcact ggcctacgtg
      841 ccaatccggt acctggagaa aggtaggatc tggtgagctt caaattgcta cagcacaggc
      901 cacaggttgg cgctttcctg gagcaacagc tacttgtcca actggaaaac gtgttacagg
      961 tggcggcgga atatgcacct cacgaactgg ttacatatgg cttaccaggt cattcccatc
     1021 agccaataac tcatggtcgg ctgcgtgcga tacaaccgaa gatcagaatg gttcaataac
     1081 tgtatatgcc atttgccaat aagctatttc aagcaagtaa tcttcataaa gtaggacgaa
     1141 tactgtgtgt agtttttggc ttgccaagtc atctcgccgt tagagtcagg catgtcaata
     1201 ggaacgactt ctgtttctgc catttcacgc ccgtcaatac gataggtgtt aatacacagt
     1261 ttaaacctcc ctaagacctg tgtgtttttg ggatataacg taaatgtttt tatattcgtc
     1321 catatcgacg ctgatgatga tttccaggta ccggattggc acgcttgcag agaatgattg
     1381 ttggcattat tttttgcctc ttttgaggtt agcatgccgt ctccacgcat ccgtaaaatg
     1441 tccctgtcac gcgcactgga taagtacctg aaaacagttt ctgttcacaa gaaagggcat
     1501 caacaggagt tttaccggag caatgttatc aagcgatatc ccattgccct tcggaatatg
     1561 gacgaaataa caaccgttga tattgctaca tacagagacg ttcgtttagc agaaatcaac
     1621 ccccgaacgg gtaaacccat tacaggtaat actgtacgtc ttgaactcgc ccttctgtca
     1681 tctctgttca atattgctcg tgttgaatgg ggaacctgtc gtactaaccc ggttgaactg
     1741 gttcgcaagc cgaaagtatc ctcgggacga gatcgccggc taacgtcttc agaagaacgt
     1801 cgcctttctc gctatttccg cgaaaaaaat ctgatgttgt atgtcatttt ccatcttgcc
     1861 cttgagacag ccatgcggca gggcgaaata ctggccttac gttgggagca cattgatttg
     1921 cgccacggtg tggctcattt acctgaaacc aaaaacggtc actcacggga tgttcctctg
     1981 tccagacgtg cccgtaactt tcttcagatg atgcccgtta atctccacgg caatgttttt
     2041 gattacaccg catccggctt taaaaatgcc tggagaatag ccacacaacg acttcgcatc
     2101 gaggacctgc attttcacga tctacggcat gaagcaataa gccgcttctt cgaactgggt
     2161 agcctgaatg taatggagat tgctgcaata tcaggacatc gttccatgaa tatgctgaaa
     2221 cggtatactc atcttcgtgc atggcaactg gtcagtaagc ttgatgcccg ccggcggcag
     2281 acacaaaaag tagcagcatg gtttgtgccg tatcctgccc atatcacgac catcgatgaa
     2341 gaaaatgggc agaaagcgca tcgtattgag atcggtgatt ttgataacct tcacgtcact
     2401 gccacaacga aagaggaagc agttcaccgc gccagtgagg ttttgttgcg tacactggcc
     2461 attgcagcac agaaaggcga acgtgtccca tctcccggag cgttacctgt taacgaccct
     2521 gactacatta tgatttgccc tctgaacccg ggcagcaccc cgctgtaaac atcattcaaa
     2581 acacatacag aaaggcgagt tgcaacaact cgccaaacac attcgatctg catgatcgaa
     2641 attattcgtt tcaccagaaa aaacaccgaa gatacaataa ccacatgagc tgaaaagact
     2701 ttttcagtcg gaggaacaat gagactgaat acgaccggta ttgcggcccg aatgatgctc
     2761 tcgctggaca aaaaagcaat cggcgaaaaa ctcatcaacg gtcgtcagat aaacccgaca
     2821 cctctgcagg tccgttaccc ccaaggggta agagaagttc tggggataat gagcgaacaa
     2881 ttagccatat ccaccgctga tttaacccga atcctgcttg aggatgctct tcataatatg
     2941 tttatgcctg cggataacac cacaggaaac ataataagca gaattgaata catcatgctg
     3001 tcgcatgata taaatgcgcc attactggca acattgctgt cgccatggaa tatccgctcc
     3061 acggttatac aggacccggc cagacttgca gactatcttt cagctgacgc tctcgaacat
     3121 cttgctgaat gttttaatct caatccggac tggctgaatg gccatgaaaa ctatccgata
     3181 gcgttgtccg gtgaatggcc agatactgct gataatttca gaatgcttat tagtgatagc
     3241 agcaacactg aagttatttt ctggcatagt ttccccttcg caggaaatac gaaaagagaa
     3301 tattgcgggg taatattgag acagaaaaaa gaaataaatg gctctgtaat atatccggca
     3361 ctatcattat caccaacact tctgaacgat gaaaaaagaa aatggttaac tgaatataca
     3421 accaggcaga atacaacaat gtctttgcga cgcgtcacgt tgagacctgg ccttgccggg
     3481 aaccttatta cagggcaaat actacctgtt agcttattta atacgtcttt attaccctgg
     3541 taaataccag atagtaccaa agtaaaaatt acggcacatc cgtgtcatag cgttgttttg
     3601 tctaaattct gataaacagg aaattaaatg atgaagaaaa atcatattac ccgcaccatc
     3661 attgcatctg ctgtattatt ttcctttaac gctgcagcag cgacgtcata ttttgaagcg
     3721 cgtaacgatg caatgggtgg taccggggta gcatcttccc attatggtgt ggcacctctg
     3781 gcaaacccgg ctttactgac aaagcacaac agcaatgatg atttcagcct tcttctgccg
     3841 tctgttgggg cacaagttgc tgatcctgat gatgtatcaa ataaagctga tgatgttaaa
     3901 gatgactggg atttgtttga cagtgctgta gataaccagc atggcgtaca acaggcagcg
     3961 gcaaatctta aacatcgtct gcaggaattc cgcaatatca atgcagatgc tcaggtaggt
     4021 gtatcagctg tagcagccat ggcaaatgac actctgccgt ttgcccttat ggtcaagtct
     4081 tacggaactg tcagcgtaaa cggaaaagta aacgatgcag acctggatta tctggacaaa
     4141 gttgccaatg gaacaattac cgacgtcgat aagaatgccc tgacctcacg cgcattcggg
     4201 cgtgcagctg taattaccga cgtcggaatt tcatttgcca aagaactgga aacggccgga
     4261 caaaaatggt ctcttggcgt cacaccaaaa tatcagcgtg tggacctgtt taactacaac
     4321 gtaacggttc gtgattacga caaagacgat tttgacggtg acaagtacca caacacgaaa
     4381 aacggattca acgccgatat tggtgcttat acagacctga atgacaactg gactgtcggt
     4441 cttgttgccc agaacatcat tccacgcagt attgatacta aagtcgtcaa tggctttaaa
     4501 gaaaccttca aagttcgtcc gcaggcaacc gccggtgtat cctggcataa tgacctgttt
     4561 accactgctc ttgacattga tctgactcct gccagtggct tcacttctga cagcaaacgt
     4621 cagtttgcaa gtgttggtgc tgaatttaac gcatggaaat gggcgcaatt gcgtgccggt
     4681 tatcgtcaga acatggcatc aaactccgga agcgccttca ccgctggcgt tggtatctct
     4741 ccatttgacg tcgtacatat cgatgtttcc ggcctggtag gaactgacca cgattacggt
     4801 gcaatggcac aacttcagtt cactttctga ttactgtccc cgcccccggc actaaacccg
     4861 ggggtatttt tatattcagt ttaaataaca tgatgaaacc acgttcttct tattcaaaaa
     4921 cagcattcat tcttttattt tctgttttcc tggtagcggc agtgacaaaa gcaaaaagct
     4981 cgcttcccga tatcaccctg gaacaggcaa aagaaattaa tgctgataac accgttattt
     5041 tccttttccg ccatggagaa cgttgcgacc gttcagatat gccatgctat tcggataaaa
     5101 gcggaatcac tattaccggt acagagaaag cacagcagga aggtattaag ttcgcaacaa
     5161 tcttcagtga atatgatatt tactccagca atgcggtaag aaccatacaa actgcaaaat
     5221 tcttctccgg taaagaaccc gtcgttatgg acagtttatc agactgcaat aatgatcttt
     5281 ataagacgct tgaatcaatc gca
//
LOCUS       DAPGOF010000251         5248 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.254, whole genome shotgun sequence.
ACCESSION   DAPGOF010000251 DAPGOF010000000
VERSION     DAPGOF010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.254"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            87..650
                     /gene="ipbA"
                     /locus_tag="RHV13_004454"
                     /gene_synonym="fimX"
     CDS             87..650
                     /gene="ipbA"
                     /locus_tag="RHV13_004454"
                     /gene_synonym="fimX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060710320.1"
                     /note="IpbA is also known as HbiF and FimX; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase IpbA"
                     /protein_id="HDV1243897.1"
                     /translation="MTRKYLTQDEVYRLMDAAQSMSFPERNRCLIMMAFIHGFRASEL
                     LDLRLSDIDASGKQLNIRRIKNGFSTTHPLLPDEYNLIKLWLKQRKLIENGVEGDWLF
                     LSRKRRPISRQHFFSIIREAGRRAGLAVKAHPHMLRHACGFALADNGVDTRLLQDYLG
                     HRNIQHTVRYTASNAARFKGVWKKKPR"
     gene            980..1774
                     /gene="hyxR"
                     /locus_tag="RHV13_004455"
     CDS             980..1774
                     /gene="hyxR"
                     /locus_tag="RHV13_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator HyxR"
                     /protein_id="HDV1243898.1"
                     /translation="MKKPLVIISACQFTRLALESLIPTDRYLVRVYSNVTTEVEFILK
                     TSCGYLLADIPSLPSGELVLLACLVRLRSDAGQWQVCLTGDSDWFDNAPAASLYYGFP
                     RISTLLTASRYQNQIIRWLLRPLPGDMSDFWLLTARELSVLKILMQGMSFSEIARYEQ
                     RSSKTLHAIATRALMKLGLGTLSDFRLLYTGCGDSRVKRNIRLHARYVGQHSAHRLTQ
                     YVQNMVSGILYPEKVAVGESINSDAISGKVLSGQRKHMSKKRPGLL"
     gene            1928..2689
                     /gene="hyxA"
                     /locus_tag="RHV13_004456"
     CDS             1928..2689
                     /gene="hyxA"
                     /locus_tag="RHV13_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein HyxA"
                     /protein_id="HDV1243899.1"
                     /translation="MRFVLFCPSGIVPAQFAALSTGSVGNVTCIRTEEELRNKLRHRP
                     QNVVISAGRPAECAEMWFRFYRDHSFVVVLCVAPFFLPPDVSISGVLKNLRLLKPGMS
                     VERVISIANASGGFSGLKHAENLPVMDSYSVFMKEVNNRTKTIVMSERFPEKQKKVLS
                     LLLAGHSWEYSAQFLKTGIRQIWLAEQSLKKRWGIPDSMSLREALLLSSNKFHGDGNA
                     LEKTNAMTLRENGNTNRYSVVVNAGTQVLSLHKYK"
     gene            2731..3813
                     /locus_tag="RHV13_004457"
                     /pseudo
     CDS             2731..3813
                     /locus_tag="RHV13_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308377.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
     gene            3835..5028
                     /locus_tag="RHV13_004458"
     CDS             3835..5028
                     /locus_tag="RHV13_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDV1243900.1"
                     /translation="MTKATVNTGTFASQNGTMIVDASSENTLDISGKASGDLRVYSAG
                     SLDLINEQTAFISTGKDSTLKATGTTEGGLYQYDLTQGADGNFYFVKNTHKASNASSV
                     IQAMAAAPANVANLQADTLSARQDAVRLSENDKGGVWIQYFGGKQKHTTAGNASYDLD
                     VNGVMLGGDTRFMTEDGNWLAGVAMSSAKGDMTTMQSKGDTEGYSFHAYLSRQYNNGI
                     FIDTAAQFGHYSNTADVRLMNGGGTIKADFNTNGFGAMVKGGYTWKDGNGLFIQPYAK
                     LSALTLEGVDYQLNGVDVHSDSYNSVLGEAGTRVGYDFAVGNASVKPYLNLAALNEFS
                     DGNKVRLGDESVNASIDGAAFRVGAGVQADITKNMGAYASLDYTKGDDIENPLQGVVG
                     INVTW"
BASE COUNT         1486 a         1117 c         1262 g         1383 t
ORIGIN      
        1 ataatgagat tacatcgata acgttctgat tgcaggcata cttatctggg tgtgcctctc
       61 ttttttacaa ctgtacacgg tggcgtatga cgagaaaata tctcacacag gatgaagtct
      121 acaggctgat ggatgcagcc cagagcatgt cctttcctga aagaaatcgc tgtctaatta
      181 tgatggcttt cattcacggt tttagggcca gtgaacttct tgatttacgt ttatccgata
      241 ttgatgcatc aggaaaacaa cttaatattc ggcggattaa aaatggtttc tcgacaacac
      301 atcctcttct tcctgatgaa tataatttga tcaagttgtg gcttaagcag aggaagttaa
      361 ttgaaaacgg agttgaagga gactggcttt ttctctcccg gaaacgccgc cctatcagca
      421 gacaacattt tttttctatc attcgtgagg ctggaagacg tgcaggatta gctgtaaaag
      481 cacatcctca tatgttacgt catgcctgtg gttttgctct ggctgacaat ggtgtcgata
      541 cccgactact tcaggattat ctgggtcatc gaaatatcca gcatacggtc agatacacag
      601 ccagtaatgc tgctcgattt aaaggggtgt ggaaaaaaaa gcctcgttaa aacagtacct
      661 tctgattcaa aatgtcagat tcctacgttt tcctcaattc ttccataaaa aaacagccca
      721 ttcaattcaa taaaacacat aacatgttga tatttctcgc taaagtaaaa taaccccatg
      781 atttgaaatg attaaccgcc aaggtcatac cagtgattaa ctttcaccca tgttgttaaa
      841 tgaaaccaaa ttaaaaaaaa taaaaatcaa aaaaaataga tttatattcc aagaaaatta
      901 aatattcagg caattaaacc aaacctgaca ttttgaatca aaatgtcagg tttcgtagca
      961 atggcgtgga cgagtctgaa tgaagaagcc tctggttatt atcagtgcct gtcagttcac
     1021 caggctggca ctggagtctc tcatcccaac ggacaggtat ctcgtgcggg tgtacagcaa
     1081 cgtcactaca gaggttgaat tcattttaaa gacctcctgt ggctacctgt tagctgatat
     1141 cccttcgctt ccttcggggg agttggttct tctggcttgt cttgtccgct tgcggagtga
     1201 tgccgggcaa tggcaggtct gcctgaccgg tgactccgac tggttcgata atgctcctgc
     1261 tgcaagtctg tactatggct tcccacgcat aagcaccctg ctgacagcga gtcgttatca
     1321 gaatcaaatt atccgctggt tactacgtcc tttgcccggc gatatgagtg atttctggct
     1381 gctgactgca cgggagttga gcgtgcttaa gattctgatg cagggcatga gtttcagtga
     1441 gattgcccgt tatgaacaac gcagttcaaa gaccctgcat gcgattgcca cacgggcatt
     1501 aatgaaactt ggactgggaa ctctgagcga tttcaggctg ctctatacgg gatgcggtga
     1561 cagcagagtg aaacggaata tacggctaca tgcaagatat gtcggacaac attccgctca
     1621 tcgactcact cagtatgtgc agaacatggt atccggaatt ctttatccag agaaagttgc
     1681 cgttggtgaa tcaataaatt ctgacgccat ttccggaaaa gtactttccg gacaaagaaa
     1741 acatatgtct aaaaaaagac caggattgtt atgacacagc agcgttgtta ctaccaccca
     1801 ctgcggcgcg tacggactcg ctacagtgta ggggccaaaa tatttctact gctctgggct
     1861 gcaggagtgg gaggcacgct gtacagcctg tacagcgtgc tggcgtactg acatctgtga
     1921 gggaaggatg cgtttcgttc ttttctgtcc ctcaggaatt gtaccagcgc agttcgcagc
     1981 tctatcaacc ggttctgtcg gcaatgttac ctgcatcaga acagaagagg agttacgcaa
     2041 taaattgcgg cacaggccac agaatgtcgt gatatcggca gggcgacctg ccgaatgcgc
     2101 tgaaatgtgg ttccgatttt atcgggatca ctcattcgtg gtggtgttgt gcgtcgcgcc
     2161 cttttttctg ccaccggatg tgagtatttc tggcgttctc aaaaacctga ggttactgaa
     2221 accaggaatg tcagtagagc gtgtcatcag tattgcaaat gcctctggtg ggttttcagg
     2281 actgaaacat gcagaaaatt tgccagttat ggacagttac tctgtcttta tgaaagaggt
     2341 gaataaccgc acaaagacaa tcgtcatgtc agaacgcttc ccggaaaagc aaaagaaggt
     2401 gttgtcgtta cttcttgccg gacacagctg ggaatacagt gcgcagtttc tcaaaacggg
     2461 gataagacag atatggcttg ccgagcagtc gctaaaaaag cgttggggaa taccggacag
     2521 catgtcattg cgggaagccc tgctgctgag cagcaataaa tttcacggtg atggaaatgc
     2581 attggaaaaa acgaatgcca tgacattgcg tgaaaacgga aataccaaca ggtattccgt
     2641 ggtggtaaat gcagggacgc aggttttatc tctgcataaa tataaataag tgggatattg
     2701 aaattagtta ccagaatgga gaatttcttc atgaaaaaca ataaggcatt ttaccgcagc
     2761 gcattagcga cagctattgt tatggctctt tctgcaccag cattcgctgc tgataacgcg
     2821 gtatcaactg attcggttac gctgaataaa gataagacga ctctggatca agatgttgtt
     2881 attagcaata cagcagacaa acagattaca gccgtaacaa ttaatgcggc agatgaagac
     2941 cttaatgttg cttttgctgg tcatgatatt actgctgaat caacggcaga caaaaaattc
     3001 gttgaaggtg taaaagttag tggtaacaaa aatgttgtga ttaatgctac agactccacc
     3061 atcacagctc aaggtgaagg cacctatgtc cggactgcaa tggtcattga ttcaactggc
     3121 gatgttgttg ttaatggcgg taatttcgtt gcaaaaaatg aaaaaggtag tgcgacaggg
     3181 atatctctgg aagggaccaa gggaaataat gtcacgctta atggcacaac cataaatgct
     3241 caaggtaata agagttccag caacgcctcc acggcaattt ttgctcaaaa gggtagctta
     3301 ttgaatagtt ttgaaggtga tgcaaccgac aacattaccc ttgctggctc aaatattatt
     3361 aatggtcgga ttgaaacaat agttactgcc gggaataaga cgggaattca tacagtcaac
     3421 ctgaatatta aggatggctc agtaattggg gcggctaata ataaacaaac aatttatgcc
     3481 tctgcttcgg cacaagtcgc aggttcagca acgcaaaatt taaatttgtc tgttgctgat
     3541 tcaaccatct actctgatgt cctggccctt tctgaaagcg agaattcagc cggtaccaca
     3601 acaaatataa atatgaacgt tgcccgctct tactgggaag gtaatgctta tacgctcaat
     3661 agcggcgata aagcgggtag tgatctggat ataaatcttt ccgatagttc agtctggaaa
     3721 ggcaaagttt caggggcagg agatgccagt gtatctctgc aaaacgggtc tgtctggaat
     3781 gttacgggtt cctcaactgt tgatgctctg gcagtaaaag acagtacggt taatatcacg
     3841 aaggctacag tcaatactgg cacgtttgct tctcaaaacg gcactatgat tgttgatgcc
     3901 tcttctgaaa acactctgga tatcagcggt aaagcgagcg gtgacttgcg tgtttacagt
     3961 gcgggttcat tggatcttat caatgaacaa acggcattta tttctaccgg caaagacagc
     4021 actctaaaag ccacaggcac aacggaaggt ggtctgtatc aatatgacct gacacaggga
     4081 gctgatggta acttttattt cgtaaaaaac acgcataaag catccaacgc cagctccgtg
     4141 attcaggcaa tggcagcagc tccggctaac gttgctaatc tgcaggctga cacgctctca
     4201 gcccgtcagg atgctgtccg tctgagcgaa aatgacaagg gcggcgtatg gattcagtac
     4261 tttggcggta aacagaaaca taccaccgcg ggaaatgcat cctatgacct ggatgtaaac
     4321 ggtgtaatgc tgggtggtga tacccgcttc atgactgaag atggtaactg gctggccggt
     4381 gtggcgatgt cttctgcgaa aggtgacatg accaccatgc agagcaaagg cgacactgaa
     4441 ggttacagct tccacgctta cctgagccgc cagtataaca acggtatctt cattgatact
     4501 gctgcacagt ttggtcacta cagcaacacg gcagatgttc gcctaatgaa tggtggcggt
     4561 accatcaaag ctgactttaa caccaatggt ttcggtgcga tggttaaagg cggttacaca
     4621 tggaaagacg gtaatggcct gtttattcag ccatatgcca aactgtctgc gctgacgctg
     4681 gaaggtgtgg attatcagct caacggcgtg gacgttcatt ctgacagcta taactctgtg
     4741 ttgggtgagg ccggtacgcg cgtgggttat gacttcgctg tgggcaacgc gagcgttaaa
     4801 ccttatctga atctggccgc actgaacgaa ttctctgatg gcaacaaagt ccgtctgggt
     4861 gatgagtctg tcaatgccag cattgacggt gcagcattcc gcgtgggcgc aggtgtacag
     4921 gctgatatca ccaaaaacat gggagcatat gcaagccttg actacaccaa aggtgacgac
     4981 attgagaacc cgctacaggg tgtagttggt atcaatgtga cctggtaaga ataaagcagt
     5041 cggttcccct gtcggctgtt ataccccctg caggcagcag tatgctgcct gcaggctata
     5101 aatatccagt attcatgatg ctggtttttt gctgatattt aacccgaaca ttcctgatac
     5161 agactattaa tacaacctta tctgcgacag atttcatttt ctggatatat tatgaatgaa
     5221 caaatcaaac aggatatcga tctgattg
//
LOCUS       DAPGOF010000252         5138 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.256, whole genome shotgun sequence.
ACCESSION   DAPGOF010000252 DAPGOF010000000
VERSION     DAPGOF010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5138
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.256"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(299..2980)
                     /locus_tag="RHV13_004459"
     CDS             complement(299..2980)
                     /locus_tag="RHV13_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraC family protein"
                     /protein_id="HDV1243901.1"
                     /translation="MEAINNYNSGLNRHQLGGFIPVYDQLAGTHYFLIDGNRLGFMFI
                     CNPSPGVFDNQQDVLAEMFKMDFPTDTVCQISLTALPDLTLQLSAWSAVRGGRMTGND
                     KLKADLLNGYQLDYYDRSMHKPLKPDHDNLMLRDFQVWISLSIPLQSALPTELEHSRI
                     DSLYSELVSKLITIGLHPFKVDAENWLYCIDKVVNPGKNSRWAEGFIDVNTMKRLNEQ
                     VNVPGRKYTVTENHFSSVTQSDDESEHRYFKQLSVVKFPEYVNFGCMYELVVNWMHGR
                     KTIFSPFMITQTVQFADPLKLSKENVRYKAITNKQASIPSVVTFCPRLRDMDNDYMAV
                     TRELEDGAKLLRGYLTFTVMGSNANSVQTAANDLKSFYLESRVKVADDSFIVFPSFMS
                     CLPMCNDPKTIFDLDRSEVVSNTGAAHMTPIFGPWKGNTDRPVLSLVSREGQLMGLDI
                     FKTSASYNMVIGATSGAGKSFWTAYLINNYLGAGPRSNNLVHYRSTFKHFLENEYPDD
                     DPDGAQVFVVDVGRSYQGIAEQYTNSQFIDFGKTPDFTLNPFAFLTDITVNDDVFNEA
                     PEFTGESTSNDAEKDKVAQTIMVLNQLKIMASEKGLIDDYQQSVMLQLIAEEYQESRK
                     SGRTGSITGFALRCKKHEDKRIKDIGEQLGAWCEGGIYGHRFTDTLPPINFDSRFIVL
                     ELEELKGTPHLQTVVLMSIIQAAQHAMFIKKDGRRRLFILDEAWEYIRPDNSSGAGNQ
                     SNQFFSSFLEAAWRRFRKTNCAGICITQSFEDYFTSSVGRALTANSPWKIIMKQEKES
                     IEAMKANKYFSTSDAEYERMKNIRTVKKVFSEMLVRFENFQEICRLYVDRKMELCFTT
                     DSADRSKLWEIQSRENCSYGEAIEILYAQEVAAGLAA"
     gene            complement(2989..3939)
                     /gene="trhV"
                     /locus_tag="RHV13_004460"
     CDS             complement(2989..3939)
                     /gene="trhV"
                     /locus_tag="RHV13_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncHI-type conjugal transfer lipoprotein TrhV"
                     /protein_id="HDV1243902.1"
                     /translation="MNIKRLVLSALVVGTSSYLTGCSIGSSESECPGIEKGVICKGPR
                     EVMELTNNRDDLSALAGEESESGKEKSAVNDSRYPTEISPPGEVKYPQSTTLKNQPVA
                     YSKTEIKPVGQLPVMYDKTLKMGAPTSSIGPRPISGVPVNSNVRMTSSYSTASSAGNP
                     FVHPAAEVVKQTSYPVSAGNAPRYVAPNSDISPGKDMYSLYNGQPVNPTLNTGQIQQY
                     RSQGYKQAVVAPEPLAVLQQGKVMRITFAPYTDDNDALNLPGYVYVNVKPQTWIAGKN
                     STSNPARIVPLEVQDAARENMQQQQKATKAVSSNGIVRQL"
     gene            complement(3949..4701)
                     /gene="htdT"
                     /locus_tag="RHV13_004461"
     CDS             complement(3949..4701)
                     /gene="htdT"
                     /locus_tag="RHV13_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010892304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide isomerase HtdT"
                     /protein_id="HDV1243903.1"
                     /translation="MAKAKEGGVSPSQISGALSKMKGYKPKDAIYIPSGGLYLFQDER
                     SQLMAVTTDGRYSLTGGSLVDVIKRKPVLTVEEIRNSYFISLDEAPFPLETVASIHLG
                     NSKLKRQAAIFLTLDCDGCMELVKKFYADRDKYRIDIVLVPSPGEPKEELRRLWCSKE
                     KGKINNLDILRWLMGSKSDIEKRLLSQKEAEECPAEPLVASLMLAGIYKLQGVPSVVR
                     QDGLAGNGIPKDFDYWLNQSVEPLLKNPFETN"
     gene            complement(4820..>5138)
                     /locus_tag="RHV13_004462"
     CDS             complement(4820..>5138)
                     /locus_tag="RHV13_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDV1243904.1"
                     /translation="GYKGFMNVYDYFNKQSQPSYKIAVLDMPELRKEFFKHHGGRTAD
                     NDRTQFEEYFRTLMKIYRDRGYLIIDATLAVTVPDSVEIVTYMELEDSSEAVQSSSYN
                     AKK"
BASE COUNT         1443 a         1114 c         1039 g         1542 t
ORIGIN      
        1 ctaataatgc cgcgctcctt tgattacctt tggtaaacaa aaatgtaatt tacgtgaatt
       61 tcatcttata aagtcagtat tgatgttata aattttgttt acttaatgcg tacacaacct
      121 aaatgcgatt tgtaattatg tcactgtttt taagccatat taattataaa accaaaattt
      181 gcagcacagt gatgaactca ttaagtcata acctcatttg tttacttaag gcatacaata
      241 aaaaaccaga ttcttatgag acatctggtt taattgtttt tgcttgaaga tatttactct
      301 acgcggccaa acccgctgct acttcttgag catagagaat ttctattgcc tcgccatagc
      361 tacaattctc gcggctttga atttcccata atttactcct gtcagcacta tccgtagtaa
      421 aacaaagctc cattttccgg tctacgtata accgacagat ctcttggaag ttttcgaacc
      481 tgactaacat ctctgaaaac acctttttga ctgtacgaat atttttcatt cgttcatatt
      541 ctgcatctga agtagaaaaa tatttattag ccttcattgc ttcaatgctt tctttttctt
      601 gtttcatgat tatcttccaa ggcgagttag cagtcagggc ccggccaaca gaagaagtaa
      661 aataatcttc aaacgactgt gtgatacaga tgccagcgca gtttgttttc ctgaacctac
      721 gccatgccgc ttcgaggaag gaagagaaga attggtttga ttgatttccg gcacctgatg
      781 aattatcagg acgaatgtac tcccaagctt catctaggat gaacaagcga cgacgcccat
      841 cttttttgat gaacattgca tgctgagcag cctgaatgat agacattaaa acaactgtct
      901 gaaggtgggg ggtgcctttt agctcctcca gttccaaaac aataaatctg ctgtcgaagt
      961 tgattggagg caacgtatcg gtaaaccggt gcccataaat tccaccttcg caccatgctc
     1021 cgagttgctc cccaatatcc tttatacgtt tgtcttcatg cttcttgcaa cgcagagcaa
     1081 agccggttat tgaaccggtc cgacctgatt ttctggattc ctggtactct tctgcaataa
     1141 gctgaagcat cactgattgt tgataatcat cgataaggcc tttctctgaa gccataattt
     1201 ttagctgatt cagaaccatt atggtctgag caactttgtc tttttctgca tcgtttgaag
     1261 ttgattcacc ggtaaattca ggtgcttcgt taaatacatc gtcgttaaca gtgatgtcag
     1321 ttaggaaggc aaagggattt agggtgaagt caggggtttt accgaaatca ataaattgac
     1381 tgtttgtata ttgttccgca atcccctggt aagaacgccc cacgtccact acaaaaactt
     1441 gagcaccatc aggatcatca tctgggtatt cgttttctaa aaaatgctta aatgttgagc
     1501 gataatgaac gaggttatta gatcgcggtc cagctccaag atagttattg attagatatg
     1561 ctgtccagaa tgacttgcct gcacctgacg ttgcaccaat aaccatgtta tagcttgcag
     1621 aagtcttgaa aatatcaagt cccatgagtt gaccttcacg agaaactaaa ctcagtactg
     1681 gtctatctgt attacctttc cagggcccaa agatcggcgt catatgagcc gctccggtgt
     1741 tgctcactac ttcggaacgg tcaaggtcga aaattgtctt tgggtcattg cacattggca
     1801 gacatgacat aaatgacggg aaaacgataa aggaatcatc tgctactttg acccggcttt
     1861 cgaggtaaaa ggatttgaga tcgttagctg ctgtctgcac agaattggca ttactaccca
     1921 taacagtgaa agtaagatac cctcttaaaa gctttgcgcc atcctcaagc tcgcgagtta
     1981 cggccatgta gtcattatcc atatcccgca ggcgagggca gaatgtgaca actgaaggta
     2041 tacttgcctg tttattagtg attgctttgt agcggacgtt ttcttttgac aacttgagcg
     2101 gatcggcaaa ctgaacagtc tgagtgatca taaatgggct gaaaattgtc ttacgtccat
     2161 gcatccagtt aactactaac tcatacatac aaccgaaatt gacgtattca ggaaacttta
     2221 caacggaaag ttgtttgaag taacgatgct cagattcgtc gtcactttgt gtgactgatg
     2281 agaaatgatt ttctgtgact gtatactttc ggccgggaac gttaacttgt tcattaagcc
     2341 ttttcatagt gttaacgtct ataaaacctt cagcccaccg agagttcttt ccggggttta
     2401 cgaccttatc tatacaatag agccagtttt ctgcatcgac cttaaacgga tgcagtccaa
     2461 tcgtaattaa cttacttaca agttcagagt aaagtgaatc gatacgcgaa tgttccagct
     2521 cggtaggaag ggcagactgt aaaggaatcg atagcgagat ccatacctga aaatccctca
     2581 acataaggtt atcatgatca ggttttaaag gcttatgcat acttctgtcg tagtagtcca
     2641 actgataacc attaagcaga tctgctttaa gcttatcgtt cccagtcata cgcccacctc
     2701 gcacagctga ccaagcacta agctgtaaag tcaagtctgg caatgcagtc agagatattt
     2761 gacagacagt atctgtagga aaatccattt tgaacatttc agcaagaaca tcttgctgat
     2821 tatcaaaaac tccaggtgat ggattacaga taaacataaa ccctaatctg ttgccatcaa
     2881 tcaaaaaata gtgagtccct gctaattgat cgtaaacagg gataaaccca cctaattgat
     2941 ggcgatttaa accgctattg taattattga tagcttccat atttgacctt acagttgtct
     3001 aacaatgccg ttcgaagaga cagctttagt tgctttttgt tgctgctgca tattttcccg
     3061 ggccgcatcc tgtacttcta aaggaactat gcgagctggg tttgaggtcg aattcttgcc
     3121 agcaatccat gtttgcggtt ttacgttgac ataaacgtag ccgggaagat taagcgcatc
     3181 gttatcatcg gtataaggtg caaaagtaat tctcattact ttcccctgtt gcagaacagc
     3241 aagtggttca ggagccacta cagcttgttt ataaccttgc gaacggtatt gctggatctg
     3301 cccagtgttg agagtagggt taacgggctg gccattgtaa agagagtaca tatccttgcc
     3361 agggctgata tcagaattag gtgcaacgta gcgtggtgca tttccagcag aaaccggata
     3421 gcttgtttgt ttaacgactt ccgcagccgg atgaacgaaa gggttaccag ctgaagaggc
     3481 agtgctataa ctgctagtca tccttacgtt tgaattaacc ggtacaccag aaataggccg
     3541 tggtccaatg gatgaagtag gggcacccat ttttaaagtt ttatcgtaca tgacaggtaa
     3601 ctgaccaact ggcttaattt cggtttttga ataagccaca ggctgatttt taagggttgt
     3661 agactgtgga tatttgactt cccctggagg gctaatttca gttggataac ggctgtcatt
     3721 gacagccgat ttctcctttc ccgattccga ctcttccccc gcaagtgcag acagatcatc
     3781 tcgattatta gttaactcca taacttcgcg aggaccttta cagataacgc ccttttcaat
     3841 gcctggacat tcggattcgg aactcccgat ggagcacccc gtaagatatg aggatgtacc
     3901 tacaaccaga gcactgagta ccaatctttt gatgttcata aactagcctt aatttgtctc
     3961 aaatggattt tttaagagag gttcaacgct ttggttcaac cagtagtcga aatcttttgg
     4021 aatgccatta ccagccaaac catcctgtct gacaactgaa ggtacaccct gcaatttata
     4081 aattcctgcg agcatcaatg acgctaccaa aggttccgcc ggacattctt ctgcttcttt
     4141 ttgagagagc aatctttttt caatgtcgga tttgcttccc attagccaac gaagaatatc
     4201 gaggttgttt atttttcctt tttctttact acaccagagc cttctaagct cttcttttgg
     4261 ctcaccgggt gagggaacta aaacaatatc tatccgatac ttgtcccgat ctgcataaaa
     4321 tttcttaaca agttccatgc aaccgtcaca atcaagtgtt aagaaaatgg ctgcttgtct
     4381 cttaagcttt gaatttccca gatgaatgga cgctacagtt tccagaggga agggggcctc
     4441 atcaagacta ataaaatagg aatttctgat ttcttctaca gtcagaacag gtttacgttt
     4501 aattacatca acaaggcttc ccccagtaag ggagtatcgg ccatcggtcg ttactgccat
     4561 tagctgcgaa cgctcgtcct gaaagaggta tagcccacca gaaggaatgt atatggcatc
     4621 cttaggttta tatcctttca tttttgataa cgcacccgag atctgactgg gtgaaacgcc
     4681 gccttccttt gcctttgcca ttaacgcagc agcatcacta ttagttagga cctgctctga
     4741 agcataagtg accgtattcg ccataatggc gaaagccgcc attaaaggga aaagtaactt
     4801 tttcactatt gggtgccctc tacttctttg cattataaga acttgattgt accgcttcag
     4861 agctatcttc aagttccata taagtgacga tttcaacact atccggtaca gtaacggcaa
     4921 gcgttgcatc aataattaaa tagccacggt cacggtagat cttcattaat gttctgaaat
     4981 attcttcgaa ttgagttcta tcattgtcag cagtacggcc tccgtgatgc ttgaaaaatt
     5041 ccttacgtaa ttcaggcatg tcaagcactg caatcttgta cgaaggctgt gattgtttat
     5101 tgaagtaatc gtaaacattc ataaagcctt tataacca
//
LOCUS       DAPGOF010000253         5119 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.257, whole genome shotgun sequence.
ACCESSION   DAPGOF010000253 DAPGOF010000000
VERSION     DAPGOF010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5119
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.257"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            79..1413
                     /gene="garP"
                     /locus_tag="RHV13_004463"
     CDS             79..1413
                     /gene="garP"
                     /locus_tag="RHV13_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HDV1243905.1"
                     /translation="MILDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            1429..2199
                     /gene="garL"
                     /locus_tag="RHV13_004464"
     CDS             1429..2199
                     /gene="garL"
                     /locus_tag="RHV13_004464"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HDV1243906.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            2229..3119
                     /gene="garR"
                     /locus_tag="RHV13_004465"
     CDS             2229..3119
                     /gene="garR"
                     /locus_tag="RHV13_004465"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HDV1243907.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            3216..4361
                     /gene="garK"
                     /locus_tag="RHV13_004466"
     CDS             3216..4361
                     /gene="garK"
                     /locus_tag="RHV13_004466"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HDV1243908.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAD
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            4394..4770
                     /gene="rnpB"
                     /locus_tag="RHV13_004467"
     ncRNA           4394..4770
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="RHV13_004467"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT         1197 a         1234 c         1462 g         1226 t
ORIGIN      
        1 ttacggcata aatgaaactc ataaataaca ccttccccat aataataaaa atcagcataa
       61 gtacccgagg taaataaaat gattctggac accgttgacg aaaaaaagaa aggcgtgcat
      121 acccgctatt taatattact gattattttt attgttaccg ccgttaacta cgccgatcgt
      181 gcaacgctgt ctattgctgg taccgaagtg gcaaaagagt tgcagttaag tgcggtttcg
      241 atgggttaca tcttctctgc ttttggctgg gcctacttgc tgatgcaaat ccccggcggc
      301 tggctgcttg ataagtttgg ctcgaaaaaa gtttacacct acagcctctt tttctggtcg
      361 ctattcacct tcctgcaagg ctttgttgat atgttcccgc tggcctgggc agggatctcc
      421 atgttcttta tgcgctttat gctcggcttc tcggaagcgc catcattccc ggcgaacgcc
      481 cgtattgtcg ccgcctggtt cccgacgaaa gaacgtggta ctgcctccgc catctttaac
      541 tcggcgcaat atttctcgct ggcgctcttt tcgcctctgc ttggctggct gactttcgcc
      601 tggggctggg agcacgtctt taccgttatg ggggtgattg gttttgtgct gacggcgctg
      661 tggatcaaat tgattcataa cccgacagat cacccacgta tgtctgcgga agagctgaag
      721 tttatctctg aaaatggcgc ggtggtcgat atggaccaca aaaagccggg cagtgcggca
      781 gcaagcggac ccaaactgca ttacatcaag caattgctct ctaaccgcat gatgttgggc
      841 gtatttttcg gacaatattt tatcaacacc atcacctggt tcttcctcac ctggttccca
      901 atttatctgg tgcaggaaaa aggcatgtcg attctgaaag tgggtctggt cgcctcgatt
      961 ccggcactgt gtggttttgc cggtggcgtg ctcggtggtg tcttctcgga ttatctgatc
     1021 aaacgcggct tgtccctgac cctggcacgt aagttaccga ttgtgctggg aatgttgctg
     1081 gcttccacca tcatcttatg taactacacc aataacacca cgctggtggt catgctgatg
     1141 gcgctggctt tctttggcaa aggatttggt gcgctgggct ggccggtaat ttctgacacc
     1201 gcgccgaaag agattgttgg cctctgcggc ggcgtcttta acgtctttgg caatgttgcc
     1261 tccattgtca ctccactggt gattggctac ctggtaagtg aactgcactc cttcaatgca
     1321 gcactggttt tcgtgggatg ttcagcgctg atggcgatgg tctgctacct cttcgtagtt
     1381 ggcgacatta aacgtatgga attgcagaaa taagcaaagg tataagcgat gaataacgat
     1441 gttttcccga ataaattcaa agccgcactg gctgcgaaac aggtacaaat tggttgctgg
     1501 tcagcactct ctaacccgat tagcactgaa gttcttggtt tggctgggtt tgactggctg
     1561 gtgctggatg gcgaacatgc gccaaacgat atctccacgt ttattccgca gttaatggcc
     1621 ctgaaaggca gcgccagcgc gccagtagtg cgagtgccga ccaacgagcc ggtaattatt
     1681 aagcgtcttc tggatatcgg tttctataac ttcctgattc cttttgtaga aacaaaagag
     1741 gaagcagagc aggcggtggc atcgacgcgt tatccgccag aaggcattcg cggcgtctcc
     1801 gtttctcacc gcgccaatat gtttggcacc gtggcggatt atttcgctca gtcgaacaag
     1861 aacatcacta ttctggtcca gatagaaagt cagcagggcg tagataacgt cgatgctatt
     1921 gccgctaccg aaggcgtaga cggcatcttc gtcggcccca gcgatctggc cgcggcatta
     1981 ggccatctcg gcaatgcctc gcacccggat gtacaaaaag caattcagca tatttttaac
     2041 cgtgccagcg cgcacggcaa acccagcggt atcctcgcgc cggtcgaagc cgatgcgcgt
     2101 cgttatctgg aatggggcgc gacgtttgtg gctgtcggca gcgatctcgg cgtcttccgc
     2161 tctgccactc agaaactggc tgataccttt aaaaaataac caccaccgca acaagagaga
     2221 tgattgatat gactatgaaa gttggtttta ttggcctggg gattatgggt aaaccaatga
     2281 gtaaaaacct tctgaaagca ggttactcgc tggtggttgc tgaccgtaac ccagaagcta
     2341 ttgctgacgt gattgctgca ggtgcagaaa cagcgtctac ggctaaagcg atcgctgaac
     2401 agtgcgacgt catcataacc atgctgccaa actcccctca tgtgaaagag gtggcgctgg
     2461 gtgagaatgg cattattgaa ggcgcgaagc caggtacggt attgatcgat atgagttcta
     2521 tcgcaccgct ggcaagccgt gaaatcagcg aagcgctgaa agcgaaaggc attgatatgc
     2581 tggatgctcc ggtgagcggc ggtgaaccga aagccatcga cggtacgctg tcagtgatgg
     2641 taggcggcga caaggctatt ttcgacaaat actatgattt gatgaaagcg atggcgggtt
     2701 ccgtggtgca taccggggaa atcggtgcag gtaacgtcac caaactggca aatcaggtca
     2761 ttgtggcgct gaatattgct gcgatgtcag aagcgttaac gctggcaact aaagcgggcg
     2821 ttaaccctga cctggtttat caggcaattc gcggtgggct ggcgggcagt accgtgctgg
     2881 atgccaaagc gccgatggtg atggaccgca acttcaagcc gggcttccgc attgatctgc
     2941 atattaagga tctggcgaat gcgctggata cttctcacgg cgtcggcgca caactgccgc
     3001 tcacagctgc ggttatggag atgatgcagg cactgcgtgc agatggttta ggaacggcgg
     3061 atcatagcgc cctggcgtgc tactacgaaa aactggcgaa agtcgaagtt actcgttaat
     3121 gacgtcgtgc ccggatggcg tattgcaatc cgggcctgga atccaggccg aataagagaa
     3181 acgccctccg gcgtcatgtg gtaacaggca taggtatgaa aatcgtaatc gccccagact
     3241 cttataaaga aagtttatct gccagcgagg ttgcgcaggc gatagaaaaa ggatttcggg
     3301 aaatttttcc tgatgcacag tacgtttctg ttcctgttgc cgacggtggc gaaggaacgg
     3361 tggaagcgat gattgcagcc acccagggtt ccgaacgtca cgcctgggtt acagggccgc
     3421 tgggcgagaa agtgaatgcc agttggggga tctccggcga tggcaaaacc gcgtttattg
     3481 agatggcggc ggccagtggg ctggagctgg tacctgcgga taaacgcgat ccactcgtga
     3541 ccacttcacg cggcacaggc gagttaatcc tgcaggcgct ggagagcggc gcgacaaaca
     3601 ttattatcgg cattggcggc agcgctacaa atgatggcgg cgcaggcatg gtacaggcgc
     3661 tgggggcgaa attatgcgac gccaacggca atgaaattgg ttttggcggc ggtagtctta
     3721 atactctgaa tgatattgat atttccggcc tcgatccgcg cttaaaagat tgcgtcattc
     3781 gcgtcgcttg tgatgtcacc aatccgctgg tgggcgataa cggcgcatcg cgcatctttg
     3841 gcccacaaaa gggagccagt gaagcgatga ttgttgagct ggacaataac ctctctcact
     3901 atgccgaggt cattaaaaaa gcactgcatg ttgatgtgaa agatgtcccc ggcgcaggag
     3961 ctgcgggtgg tatgggcgcg gcgctaatgg cgtttcttgg tgcggaactg aaaagtggta
     4021 ttgaaatcgt cactacggcg ctgaatctgg aggagcatat tcacgattgt acgctggtga
     4081 tcaccggtga agggcgtatt gacagccaga gtattcacgg caaggtaccg attggcgtcg
     4141 caaacgtggc gaagaagtac cataaaccgg tgattggcat tgcgggtagc ttgaccgatg
     4201 atgttggcgt tgtacatcag catggcattg atgcggtctt cagcgtactg accagcattg
     4261 gtacgttgga cgaagcattc cgcggggctt atgacaatat ctgccgtgct tcacgtaata
     4321 tcgccgcgac actggcgatt ggaatgcgca acgcggggtg acaagggcgc gcaaaccctc
     4381 tatactgcgc gccgaagctg accagacagt cgccgcttcg tcgtcgtcct cttcggggga
     4441 gacgggcgga ggggaggaaa gtccgggctc catagggcag ggtgccaggt aacgcctggg
     4501 ggggaaaccc acgaccagtg caacagagag caaaccgccg atggcccacg caagtgggat
     4561 caggtaaggg tgaaagggtg cggtaagagc gcaccgcgcg gctggcaaca gtccgtggca
     4621 cggtaaactc cacccggagc aaggccaaat aggggttcat aaggtacggc ccgtactgaa
     4681 cccgggtagg ctgcttgagc cagtgagcga ttgctggcct agatgaatga ctgtccacga
     4741 cagaacccgg cttatcggtc agtttcacct gatttacgta aaaacccgct tcggcgggtt
     4801 tttgctttta gaggggcaga aagatgaatg actgtccacg acgctagacc caaaagaaag
     4861 cggcttatcg gtcagtttca cctcatgtac gtaataaatc gttccggcgg gtttcagact
     4921 gttgagtgcg ctttattcat gccggatgcg gcgtgaacgc cttatcctgc ctacaaaagc
     4981 acgcaaattc aatatattgc agagatggcg taggcctgat aagcgtagcg catcaggcat
     5041 ttttgcggtt gccatcagtt tcaaacccgc gtcgataagt ttttgctttt ggtgcacagg
     5101 gtaagatgaa tatctacgg
//
LOCUS       DAPGOF010000254         5113 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.258, whole genome shotgun sequence.
ACCESSION   DAPGOF010000254 DAPGOF010000000
VERSION     DAPGOF010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.258"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(419..1051)
                     /locus_tag="RHV13_004468"
     CDS             complement(419..1051)
                     /locus_tag="RHV13_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HDV1243909.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            1347..1622
                     /gene="ypdI"
                     /locus_tag="RHV13_004469"
     CDS             1347..1622
                     /gene="ypdI"
                     /locus_tag="RHV13_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HDV1243910.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(1699..1941)
                     /locus_tag="RHV13_004470"
     CDS             complement(1699..1941)
                     /locus_tag="RHV13_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HDV1243911.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            2294..3214
                     /gene="lpxP"
                     /locus_tag="RHV13_004471"
     CDS             2294..3214
                     /gene="lpxP"
                     /locus_tag="RHV13_004471"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HDV1243912.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            3570..3641
                     /gene="ypdK"
                     /locus_tag="RHV13_004472"
     CDS             3570..3641
                     /gene="ypdK"
                     /locus_tag="RHV13_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HDV1243913.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(3706..4944)
                     /gene="alaC"
                     /locus_tag="RHV13_004473"
     CDS             complement(3706..4944)
                     /gene="alaC"
                     /locus_tag="RHV13_004473"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HDV1243914.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
BASE COUNT         1343 a         1231 c         1151 g         1388 t
ORIGIN      
        1 tgcactcgat attttcacat tcctgtatca agtgctgaaa atcatttcat cgctacttcc
       61 aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atattggttc
      121 gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc
      181 ggtgatttac aagtaagggt atgattacgc tttttaaagc gtaccataaa atataacata
      241 ataaatgatt tgtgatgaca cgggattatt acggtttact ttgaggaaga taaaatgcga
      301 gtttatagca ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat
      361 aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt
      421 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
      481 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
      541 gagtctgttt aagcggcatc agatattggt tttccattac accaactccg gccaggcgaa
      601 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg
      661 cggcttcttt ttcgggtgtc gctacataac tctcagaaat tccgacagat gcattaatga
      721 caatatattg gtcatcattg agattggtct ttttacctgg cttagcgaac tttgcccatt
      781 cggtactgtc atctgacaac aaagctgaag cttcattggt cagttctttt gccttttccg
      841 gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt
      901 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgaacttgc tgagtttgct
      961 gttgtgccgc taccggtgca ttatcagcag cccatacagt tgaagatgcc agaattgctg
     1021 tgaccatcgt agccataatt aaacgtttca tacttacctc attctttaac agttttagaa
     1081 aacgccgcca tcatattggg tgagcatgaa atgagaaatc agagagcaac ggtcattttg
     1141 aaagggttat cttacagttg tagttattat cgcaagaagt attttagtct tacaattgta
     1201 ggaatctgct tcctgtcgca gagaactatt cttatgtacc acaaagagat gtcatttatt
     1261 atatcgatgt aaatattgat taattatatg taaatacagc cataacatcg taggttctca
     1321 gtgaaccatt ttcaggataa ctttctatga aagtaaactt aatacttttc agcttatttt
     1381 tattggtctc tattatggca tgcaatgttt ttgcattttc catttctggt ggtgtaagtg
     1441 agaggagcta taaagagact gaaaaaacat cagcgatgac aaccacacac tctacaaaac
     1501 ttcagccatc acaggcgatt ttgtttaaga tgagagaaga tgcgccacca ttaaacctca
     1561 cagaagaaat cacgcctact tatccgacaa aggcgaatta tcttattcat cctgtgcgat
     1621 agcgtctgtc acatggcagg tgaacaatca tgtctgatgc tgatgtgaac acctgcccaa
     1681 atgagccatc ctgacccact atcgcctgcc ataccccacc ataaagcgaa tcatcaagcc
     1741 acaagccggg gcaagaaaca taaatatccc cgtcactagt tcattaccgc cagttaacca
     1801 catggtgaag taataaatga gtgcagcgag gatcaccatg ccgaagaaag aagagaccgc
     1861 cgataccacg ttcagtacct gaccgatgta gccgcttacg cagacaatac acagggcgag
     1921 aaacatccat aaataaatca tagctcaacc tcctgccgcg ctaaaaaacc tgtaacttca
     1981 gtcactttaa tagtaagaca atccttaacc tctggcaatt tttgctaagc atcaatgaaa
     2041 acagcatgtt aaatgcaaga ctgttgtgta cggaaaaata tttactttgc acgattaata
     2101 atcagtagct gaaagcagtc agcgagtgat aaagtacaga taccgcattc atgctcctcg
     2161 tggttatgtc ctgactagtc tttacactct ttacaggaac cattgtcgta catgatggcc
     2221 caaccaattg agtgtaccgc tgtcgacatg gaatcttctg cgatacaaca attcgtatct
     2281 acagaaggta actatgtttc cacaatgcaa attttcccgc gagtttctac atcctcgcta
     2341 ctggctcaca tggtttgggc ttggtgtact ctggctttgg gtacagcttc cttatcctgt
     2401 tctctgcttt ctcggcacgc gtattggcgc aatggcgcga ccattcctga aacgtcgtga
     2461 atctatcgcc cgtaaaaacc tggaactttg tttcccgcag cattctgcgg aagaacgcga
     2521 gaagatgatt gccgaaaact ttcgttcact cggcatggcg ctggtagaaa ccggcatggc
     2581 atggttctgg cccgacagtc gcgtacgtaa atggtttgat gttgaagggt tggataacct
     2641 taaacgcgca caaatgcaaa atcgcggcgt aatggttgtc ggcgtccatt ttatgtcgct
     2701 ggaactgggc ggccgcgtga tgggactgtg ccaaccaatg atggctacct atcgtccaca
     2761 taataatcag ctgatggaat gggtgcagac ccgtgggcgc atgcgctcta acaaagcgat
     2821 gatcggcaga aataatctgc gcggcattgt cggtgcactg aagaaaggtg aagcggtatg
     2881 gtttgctccc gatcaggatt atggtcgtaa aggcagctcc ttcgcgccgt tctttgcggt
     2941 ggaaaatgtc gccacaacca atggcaccta tgttctctcc cgtctctctg gcgcagccat
     3001 gttgaccgta acgatggtaa gaaaagcgga ttacagcgga tatcgtttgt tcatcacccc
     3061 agagatggaa ggctacccga cagatgaaaa tcaagccgct gcctatatga acaagattat
     3121 cgagaaagag atcatgcgcg caccggagca gtacctctgg atccaccgtc gctttaaaac
     3181 gcgcccggtg ggagaatcgt cgttgtacat ttaacaaact gctcttactc tttccctcct
     3241 caaatcggcc ataataacct catggttttt atggcttttt tcttaaggtt ttttccttcg
     3301 ctgaatatct aattattacc gtcagaaatt aaactgtcgc cgatatgaga cacttgtaag
     3361 ttactaaaag taaataagaa aatgtctctt gtctcgtttc agttttaggc gtacattagc
     3421 gccgtctcga gcagtttagc tcagaattct ggggtgatgc ccacggtaaa atacagggct
     3481 tttaaggtcg ctttgcccgg tgtcaactca ctgtatcagg tgtaatgaag tcattcaggc
     3541 gtaacagtaa ttacgcggag agatgtaaag tgaaatattt ctttatgggc atttctttta
     3601 tggtcatcgt ttgggccggt acttttgccc tgatgatcta aaagcaaact gtgcaatgaa
     3661 aaacaggagc catcaggctc ctgtttgcat ttctgacgac ggtgtttatt ccgcgttttc
     3721 gtgaatatgt ttgctgctgg cgggtagtaa accgtcggca cggaacatcg ctttaatccc
     3781 acgaatcgcc tgacgaatac ggtcgcggtt ttcaatcagt gcaaagcgaa catgcgtatc
     3841 accatagtcg ccaaagccaa tccccggcga gacacaaacc ttcgcttcgt taaggagctt
     3901 cttggcaaat tccagcgatc ccatggccgc atatggttcc gggattttcg cccagacata
     3961 catcgaagcc ttcggcattt caaccatcca gcccgcttca tgcagccctt taaccaatac
     4021 atcacggcgg cgtttgtact gttcggcaat gtcgcgcacg cactgttgat cgccctccag
     4081 cgccgcaatc gccgccacct gcaacggcgt aaaggtgccg taatcgtgat agcttttaat
     4141 acgagccagg gcgctgacca gcgttttgtt gccaaccata aagccgatac gccagcccgc
     4201 catgttgtag cttttcgaca gcgtaaagaa ctcgactgcc acatcgcgtg cacccggtac
     4261 ctgcatgatt gacggcgctt tccagccatc gtagacgata tcggcatagg ccaggtcgtg
     4321 gaccaccagc acatcgtagc gtttcgccag cgccacaacc ttttcgaaga actccagctc
     4381 cacgcattgc gcggttggat tcgacgggaa gccgaggatc atcatcttcg gtttcggata
     4441 actttcacga atggcgcgct ccagttcgtt gaagaaatcg acaccttcca ccagcggcac
     4501 tgagcgtacc tgcgccccgg caatcaccgc gccataaata tggatcgggt aacttggatt
     4561 cggcaccagc accgtgtcac catgatccag cgtcgccagc atcagatgcg ccaggccctc
     4621 tttcgaacca atagtgacga tggcttctga ttccgggtcg atttcaacgt cgtagcgatc
     4681 ctgataccag cgggaaatgg cgcgacgtaa ccgcggaatg ccgcgtgaag tggagtaacc
     4741 atgcgtgtcc gggcgctggg ccacagtaca taatttttcg acgatatgcg gcggagtcgc
     4801 accgtccggg ttacccatgc tgaaatcgat aatatcttcg ccgcgccgac gcgcagccat
     4861 tttcagttca gcggtgatgt taaaaacgta gggcgggaga cgatcaatgc gcgtaaagcg
     4921 acgttcaggg cgagtgtcag ccataatttc ctcagattaa cgttagcgcc cggaccgtcc
     4981 gagcgacgct gccacaatgg tggctgtttt gaaaatagcc tgattaattt ctgtctgtcc
     5041 agcgttttag aacattttta ttaatgacgt taaatgagga actctgcttt aaaaacagga
     5101 aatttatctg cgc
//
LOCUS       DAPGOF010000255         5073 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.259, whole genome shotgun sequence.
ACCESSION   DAPGOF010000255 DAPGOF010000000
VERSION     DAPGOF010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.259"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            195..1340
                     /locus_tag="RHV13_004474"
     CDS             195..1340
                     /locus_tag="RHV13_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C beta-lactamase CMY-2"
                     /protein_id="HDV1243915.1"
                     /translation="MMKKSLCCALLLTASFSTFAAAKTEQQIADIVNRTITPLMQEQA
                     IPGMAVAVIYQGKPYYFTWGKADIANNHPVTQQTLFELGSVSKTFNGVLGGDAIARGE
                     IKLSDPVTKYWPELTGKQWQGIRLLHLATYTAGGLPLQIPDDVRDKAALLHFYQNWQP
                     QWTPGAKRLYANSSIGLFGALAVKPSGMSYEEAMTRRVLQPLKLAHTWITVPQNEQKD
                     YAWGYREGKPVHVSPGQLDAEAYGVKSSVIDMARWVQANMDASHVQEKTLQQGIALAQ
                     SRYWRIGDMYQGLGWEMLNWPLKADSIINGSDSKVALAALPAVEVNPPAPAVKASWVH
                     KTGSTGGFGSYVAFVPEKNLGIVMLANKSYPNPVRVEAAWRILEKLQ"
     gene            1434..1967
                     /locus_tag="RHV13_004475"
     CDS             1434..1967
                     /locus_tag="RHV13_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HDV1243916.1"
                     /translation="MRILPVVAAVTAAFLVVACSSPTPPKGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLDKVTATYSLRDDGGINVINKGYNPDREMWQKTEGKAYFTGDPSRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEMKQQMLAIAT
                     REGFEVNKLIWVKQPGA"
     gene            complement(1964..2281)
                     /gene="sugE"
                     /locus_tag="RHV13_004476"
     CDS             complement(1964..2281)
                     /gene="sugE"
                     /locus_tag="RHV13_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDV1243917.1"
                     /translation="MSWIVLLIAGLLEVVWAIGLKYTHGFTRLTPSIITIAAMIVSIA
                     MLSWAMRTLPVGTAYAVWTGIGAVGAAITGILLLGESASPARLLSLGLIVAGIIGLKL
                     STH"
     gene            3009..4295
                     /locus_tag="RHV13_004477"
                     /pseudo
     CDS             3009..4295
                     /locus_tag="RHV13_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606836.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4165 domain-containing protein"
     gene            complement(4442..5044)
                     /locus_tag="RHV13_004478"
     CDS             complement(4442..5044)
                     /locus_tag="RHV13_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proQ/FINO family protein"
                     /protein_id="HDV1243918.1"
                     /translation="MGKNQEERKTPVIVVKKRRTFSPPSLSEKTDIIAPVFTEQTAES
                     APAGINSSAVETHIPEAPARKKKKKKRRFPRPSHWTREYTHECVEKIKILFPHLRAEG
                     GGFIPLKIGINNDISAFLAEHPETELTMDEWLCAVSCITSRRVYLQRTAVAGVPRYGL
                     DGHPKGQVSESEAQSAGRRLVTLEQKLLRMQAQQENISGQ"
BASE COUNT         1278 a         1258 c         1230 g         1307 t
ORIGIN      
        1 caatgtgtga gaagcagtct aaattcttcg tgaaatagtg atttttgaag ctaataaaaa
       61 acacacgtgg aatttaggaa aaacttatat ctgctgctaa atttaaccgt ttgtcaacac
      121 ggtgcaaatc aaacacactg attgcgtctg acgggcccgg acaccttttt gcttttaatt
      181 acggaactga tttcatgatg aaaaaatcgt tatgctgcgc tctgctgctg acagcctctt
      241 tctccacatt tgctgccgca aaaacagaac aacagattgc cgatatcgtt aatcgcacca
      301 tcaccccgtt gatgcaggag caggctattc cgggtatggc cgttgccgtt atctaccagg
      361 gaaaacccta ttatttcacc tggggtaaag ccgatatcgc caataaccac ccagtcacgc
      421 agcaaacgct gtttgagcta ggatcggtta gtaagacgtt taacggcgtg ttgggcggcg
      481 atgctatcgc ccgcggcgaa attaagctca gcgatccggt cacgaaatac tggccagaac
      541 tgacaggcaa acagtggcag ggtatccgcc tgctgcactt agccacctat acggcaggcg
      601 gcctaccgct gcagatcccc gatgacgtta gggataaagc cgcattactg catttttatc
      661 aaaactggca gccgcaatgg actccgggcg ctaagcgact ttacgctaac tccagcattg
      721 gtctgtttgg cgcgctggcg gtgaaaccct caggaatgag ttacgaagag gcaatgacca
      781 gacgcgtcct gcaaccatta aaactggcgc atacctggat tacggttccg cagaacgaac
      841 aaaaagatta tgcctggggc tatcgcgaag ggaagcccgt acacgtttct ccgggacaac
      901 ttgacgccga agcctatggc gtgaaatcca gcgttattga tatggcccgc tgggttcagg
      961 ccaacatgga tgccagccac gttcaggaga aaacgctcca gcagggcatt gcgcttgcgc
     1021 agtctcgcta ctggcgtatt ggcgatatgt accagggatt aggctgggag atgctgaact
     1081 ggccgctgaa agctgattcg atcatcaacg gcagcgacag caaagtggca ttggcagcgc
     1141 ttcccgccgt tgaggtaaac ccgcccgccc ccgcagtgaa agcctcatgg gtgcataaaa
     1201 cgggctccac tggtggattt ggcagctacg tagccttcgt tccagaaaaa aaccttggca
     1261 tcgtgatgct ggcaaacaaa agctatccta accctgtccg tgtcgaggcg gcctggcgca
     1321 ttcttgaaaa gctgcaataa ctgacgatga ggcccaggat attgggcctc ctttctttct
     1381 ctttttttcc tgttgtcatc tacacttaac aaaaatacag caaggaaaat cccatgcgca
     1441 ttttgcccgt cgttgctgca gttacggctg cattcctggt tgtcgcgtgt agctccccga
     1501 caccgccgaa aggcgttacc gtggtaaata actttgatgc caaacgctat ctgggaacct
     1561 ggtatgaaat tgcgcgcttc gaccatcgtt tcgagcgcgg attggataaa gtgaccgcaa
     1621 catacagctt gcgcgacgac ggcggcatca acgttattaa caagggctat aaccctgaca
     1681 gggagatgtg gcagaaaacg gaagggaaag cctatttcac cggcgaccca agcagagccg
     1741 cgcttaaggt ttcttttttc ggccccttct atggcgggta taacgtaatt gcactcgacc
     1801 gggaatatcg tcacgcgctg gtttgtggtc cggatcgcga ctacctgtgg atcctttcac
     1861 ggacccctac tatttcagat gaaatgaaac agcaaatgtt agccatcgcg acccgggaag
     1921 ggtttgaagt gaataaactg atttgggtga aacagcctgg cgcttagtga gtgctcagct
     1981 tcagaccaat aatgccagca acgatcagcc caaggctcag caaacgtgcc gggctggcag
     2041 actcacccag cagcaaaatc cctgtaatgg ccgccccaac agcgccaata ccggtccaga
     2101 ccgcataagc ggttcctaca ggcaacgtgc gcattgccca agagagcatg gcgatactga
     2161 cgatcatcgc cgcaatagtg ataatgcttg gcgtaagacg cgtaaaaccg tgggtgtatt
     2221 tcaggccaat cgcccagaca acttcgagca aacctgcaat taataaaacg atccaggaca
     2281 tatcaggctc cagaacaatg gggccgtccc cggtgaaaga agcgtttgca ggtcgtcctg
     2341 caaagctaat gtgtgaaatg gcatttttgc ccggaagaaa atgaatttca acctttttat
     2401 tcaccgcctg ctaaaagcaa gaattaagca taattagcgg cgtagttccc gcatttggca
     2461 ctcatcggag ttctctatga tgaagggctt tctgaaacca gaaagtcgat tgcccctctt
     2521 ctgaatgcga aacgtttatg cttaaaattt tagtgattga ccggtgccac tttacccgca
     2581 cagggataga ggccttgctt aatcattctg gcaggttcag ctcctcattt ctggtatcag
     2641 gaatcaataa tctcctgtta gcaaaagagc atattttgca atggaaaccg catctggtga
     2701 tcgcggattt atacagtttt attagcgaga cgcactccag tccgccaatt aaaccttttt
     2761 ttatgagttg tggcgtcatc ccactaattt tactgcaatc agcagacaga cagcatgcac
     2821 ccatcgtgcc ctctcaatcc gtagcccatt cggtcttgac caagcacacc acgctgaaca
     2881 cactctccca cacaattcag gatttcctta tttgctcaaa aagggcactt ccacagccca
     2941 tgtttatgca ctccctcccg taacctccag cattgggttc ttgtaactca aaacgaatgg
     3001 agaatgcgat gatcaatttc aagcctaaaa ttccggcgat gcttggggct ctggcggttc
     3061 ttaccgctgg tgctgctcat gcagagctgc tggaatacac ctttaaggct ccagatggta
     3121 cgcaacggtc tctgactccg aatgccaact atgccaaccc aacgggcaat atctcgtttg
     3181 ccttgagtgc cggtatcgac cgaaaggtaa agatttcggt gcttcggtcg gatggaacag
     3241 tggtttcgac agcgaccagc caccttcttg gggctactga tcgcatcaca gtgggtggaa
     3301 aatcctacta tggtgcggaa cttcaactac cagctccagt tggtggggca tacactatca
     3361 gggcggaaat tcttgcctct gatggttcta cagtacagac ggatgaatac cctcttactg
     3421 tcgatgtgac gccgccaacc tactcttcgc ttgccccggt ttatagtaac tatgggcagg
     3481 ttactagcgg tgatgtttgg aaactgggtc taggtggctc tgaggataat gcctttttgc
     3541 tgtcaggaat ctctgatgag tcacccatca aaggcgtcaa agcaaagctg tatcgtcaag
     3601 acggttcact ctacaaagat gtatccgtga actacgatga cgctaacgga caagctagac
     3661 agtctttcca atctggcttt ttcccagcct ctgacttgga tgaagtgttt acacttcagt
     3721 ttgagataag cgatagcgct ggaaatagct atctttcacc tcgtcaaaaa gtgatgtttg
     3781 atagcataac caatgctccg tctgcacctt ttggtgttta tgacccttca agtacaaaca
     3841 acttaggccc tggattaacc ggatttgttg cttacactga gggcatgaca gttaaaacta
     3901 acccaatcaa gttagcttgg cgtgttccgc gtgataactg gcatgagtat agagaaggtg
     3961 ggataaatat gaccaatgcc ttgggagaga tgtccaaggt aggagaagat gccagctatg
     4021 tttatctcgt cacaaccgca ccttatggca ataccgacgg caattattgg cgttgggtga
     4081 acttcggcca gtggggggga ggcggtattg cttataacct cacactctct ccatcagcgc
     4141 ctaaaagtcc cagacttttg ggtgtggact acaattacag tgatatagga tggtctagtt
     4201 tttaccgcta ctgggtgaac aattctgttc ttcctgtcac tgttagtagt atccgtgtca
     4261 aagttgaacc aaggccttac gttcaaactg ccgttctaaa aaagtatata caggcttcag
     4321 gcgtctgtat gcctgttcat gattattcag gatgaattga attttttctg cacctgattt
     4381 actcataata attctcttcc catttttctg atacgtaatg tccagctgtt atcaggagtg
     4441 tttattgccc agaaatattc tcctgctgtg cctgcatcct gagcaacttt tgttctaatg
     4501 tcaccagtct gcgaccggca ctctgtgctt cagattctga gacctgccct ttggggtgac
     4561 catccagacc ataacgtggg acgccggcga cagctgtccg ctgcagatat acacggcgtg
     4621 aggtgatgca ggacactgca cagagccatt cgtccatggt cagttctgtc tccgggtgtt
     4681 ctgccagaaa tgctgaaata tcattgttta tgccaatttt caggggaata aatccaccac
     4741 cttcagcacg aagatgagga aacaggatct ttattttttc cacgcactcg tgagtatact
     4801 cacgggtcca gtgggaggga cgtgggaaac ggcgtttttt ctttttcttc ttcctggctg
     4861 gcgcttcggg gatatgtgtt tcgacggcag atgaatttat tccggcgggg gctgattctg
     4921 ccgtctgttc agtaaataca ggtgcgataa tatctgtttt ttcggataag gacggtggcg
     4981 aaaaagtacg acgttttttc accacaatga cgggagtttt cctttcttcc tgattttttc
     5041 ccataaatac ctcggctggc tggttttact ctt
//
LOCUS       DAPGOF010000256         4832 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.260, whole genome shotgun sequence.
ACCESSION   DAPGOF010000256 DAPGOF010000000
VERSION     DAPGOF010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4832
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.260"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            12..680
                     /gene="fimC"
                     /locus_tag="RHV13_004479"
     CDS             12..680
                     /gene="fimC"
                     /locus_tag="RHV13_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HDV1243919.1"
                     /translation="MLIIFYLIISASAHAAGGIALGATRIIYPADAKQTAVWIRNSHT
                     NERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAADRESLFWMN
                     VKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSRSGNYINVH
                     NPSPFYVTLVNLQVGSQKLGNAMAAPRVNSQIPLPSGVQGKLKFQTVNDYGSVTPVRE
                     VNLN"
     gene            711..3320
                     /locus_tag="RHV13_004480"
     CDS             711..3320
                     /locus_tag="RHV13_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HDV1243920.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKSVPDATINFDFAAMRLNITIPQIAL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGVRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDEPIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQAAYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGGKPVPF
                     GSLVRENSTEITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            3333..4340
                     /gene="fimH"
                     /locus_tag="RHV13_004481"
     CDS             3333..4340
                     /gene="fimH"
                     /locus_tag="RHV13_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrin D-mannose specific adhesin FimH"
                     /protein_id="HDV1243921.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            4351..>4832
                     /gene="sfmF"
                     /locus_tag="RHV13_004482"
     CDS             4351..>4832
                     /gene="sfmF"
                     /locus_tag="RHV13_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HDV1243922.1"
                     /translation="MRRVFFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDANLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     "
BASE COUNT         1243 a         1059 c         1198 g         1332 t
ORIGIN      
        1 taaagttatt gatgctcatt atattttatt taatcatttc ggccagcgcc catgctgccg
       61 gagggatcgc attaggtgcc acgcgtatta tttatcccgc tgatgctaaa cagactgcgg
      121 tatggattag aaatagccat accaatgagc gttttctggt caattcgtgg attgaaaaca
      181 gcagcggtgt aaaagaaaag tcattcatca ttacaccgcc actgtttgtt agtgaaccca
      241 aaagcgaaaa tactttgcgt attatttaca ccggtccacc gctggcagca gatcgtgagt
      301 ctctgttctg gatgaatgtt aagacgatcc cttcggtaga taaaaatgca ttgaacggca
      361 ggaatgtttt gcaactggcg attttatcgc gcatgaaatt atttctccgt ccaattcaat
      421 tacaagaatt acccgcagaa gcgccggaca cactcaagtt ttcgcgatcc ggtaactata
      481 tcaatgttca taatccatca cctttttatg tcaccctggt taacttacaa gtgggcagcc
      541 aaaagttggg gaatgctatg gctgcaccca gagttaattc acaaattccc ttaccctcag
      601 gagtgcaggg aaagctgaaa tttcagaccg ttaatgatta tggttcagta accccggtca
      661 gagaagtgaa cttaaactaa ccgaatcatc tgacaatatc agagctaatt atgaaaatac
      721 ccactactac ggatattccg cagaggtata cctggtgtct ggccggaatt tgttattcat
      781 ctcttgccat tttaccctcc tttttaagct atgcggaaag ttatttcaac ccggcatttt
      841 tattagagaa tggcacatcc gttgctgatt tatcgcgctt tgagagaggc aatcatcaac
      901 ctgcgggagt gtatcgggtg gatctctggc gtaatgatga gttcattggt tcacaggata
      961 tcgtatttga atcgacaaca gaaaatacag gtgataaatc aggtgggtta atgccctgtt
     1021 ttaaccaggt actccttgaa cgaattggcc ttaatagcag tgcatttccc gagttagccc
     1081 agcagcaaaa caataaatgc atcaatttac tgaaatctgt acctgatgcc acaattaact
     1141 ttgattttgc agcgatgcgc ctgaacatca ctattcctca gatagcgttg ttgagtagcg
     1201 ctcacggtta cattccgcct gaagagtggg atgaaggtat tcctgcttta ctcctgaatt
     1261 ataatttcac cggtaacaga ggtaatggta acgatagcta tttttttagt gagctcagcg
     1321 ggattaatat tggcccgtgg cgtttacgca acaatggttc ctggaactat tttcgcggaa
     1381 atggatatca ttcagaacag tggaataata ttggcacctg ggtacagcgc gccattattc
     1441 cgctgaaaag tgaactggta atgggagacg gcaatacagg aagtgatatt ttcgatggtg
     1501 ttggatttcg tggtgtacgg ctttattctt ccgataatat gtatcctgat agccagcaag
     1561 ggtttgcccc aacggtacgt gggattgccc gtacggcggc ccagctaacg attcggcaaa
     1621 atggttttat tatctatcaa agctatgttt cccccggcgc ttttgaaatt acagatttgc
     1681 acccgacatc ttcaaatggc gatctggacg tcaccatcga cgaacgcgat ggcaatcagc
     1741 agaattacac tattccgtat tcaacagtgc caattttaca acgcgaaggg cgtttcaaat
     1801 ttgacctgac ggcgggcgat tttcgtagcg gtaatagtca gcaatcatcg cctttctttt
     1861 ttcagggcac ggcactcggt ggtttaccac aggaatttac tgcctacggc gggacgcaat
     1921 tatctgcaaa ttacaccgcc tttttgttag ggctggggcg caacctcggg aactggggcg
     1981 cagtgtcgct ggatgtaacg catgcgcgca gtcagttagc cgacgacagt cgtcatgagg
     2041 gggattccat tcgcttcctc tatgcgaaat caatgaacac cttcggcacc aattttcagt
     2101 taatgggtta ccgctattcg acacaaggtt tttataccct tgatgatgtt gcgtatcgtc
     2161 gaatggaggg gtacgaatat gattacgatt acgacggtga gcatcgcgat gaaccgataa
     2221 tcgtgaatta ccacaattta cgctttagcc gtaaagaccg tttgcagtta aatatttcac
     2281 aatcacttaa tgactttggc tcgctttata tctctggtac ccatcaaaaa tactggaata
     2341 cttcggattc agatacgtgg tatcaggtgg ggtataccag cagctgggtt ggcatcagtt
     2401 attcgctctc attttcgtgg aatgaatctg tagggatccc cgataacgaa cgtattgtcg
     2461 gacttaatgt ttcagtgcct ttcaatgttt tgaccaaacg tcgctacacc cgggaaaatg
     2521 cgctcgaccg cgcttatgcc tcctttaacg ccaaccgtaa cagcaacggg caaaatagct
     2581 ggctggcagg tgtaggtggg accttactgg aaggccacaa cctgagttat cacgtaagcc
     2641 agggtgatac ctcgaataat gggtacacgg gcagcgccac ggcaaactgg caggccgctt
     2701 acggtacgct gggggtcggg tataactacg accgcgatca acatgacgtt aactggcagc
     2761 tgtctggcgg tgtggtcggg catgaaaatg gcataacgct gagccagcct ttaggggata
     2821 ccaatgtttt gattaaagcg cctggcgcag gcggtgtacg cattgaaaat caaactggca
     2881 ttttaaccga ctggcgcggc tatgcggtga tgccgtatgc cacggtttat cggtataacc
     2941 gtatcgcgct tgataccaat acgatgggga attccatcga tgttgaaaaa aatattagca
     3001 gcgttgtgcc gacgcaaggc gcgttggttc gtgccaattt tgatacccgc ataggcgtgc
     3061 gggcgctcat taccgttacc cagggcggaa aaccggtgcc gtttggatca ctggtacggg
     3121 aaaacagtac cgaaataacc agtatggtgg gtgatgacgg gcaagtttat ttaagtggtg
     3181 cgccattgtc tggtgaatta ctggttcagt ggggagacgg cgcgaactca cgctgcattg
     3241 cgcactatgt attgccgaag caaagcttac agcaagccgt cactgttatt tcggcagttt
     3301 gcacacatcc tggctcataa aggaaattat caatgaagat aatctgcaga ttattattgg
     3361 caatggcatg tttgtgtctg gcaaacatat cctgggctac tgtttgtgca aatagtactg
     3421 gcgtagcaga agatgaacac tatgatctct caaatgtctt taatagcacc aataatcagc
     3481 cagggcagat tgttgtttta ccggaaaaat ccggctgggt aggtgtgtca gcaatttgtc
     3541 cacccggcac gctggtgaat tatacatacc gtagttatgt caccaacttt attgttcagg
     3601 aaactatcga taattataaa tatatgcaat taaatgatta tctattaggt gcgatgagtc
     3661 tggttgatag cgtgatggat attcaatttc ccccgcaaaa ttatattcgt atgggaacag
     3721 atcctaacgt ttcgcaaaac cttccgttcg gggtgatgga ttctcgtttg atatttcgtt
     3781 taaaggttat tcggcccttt attaacatgg tggagatccc cagacaggtg atgtttaccg
     3841 tgtatgtgac atcaacgcct tacgatccgt tggttacacc tgtttatacc attagttttg
     3901 gtggccgggt tgaagtaccg caaaactgcg aattaaatgc cgggcagatt gttgaatttg
     3961 attttggtga tatcggcgca tcgttattta gtgcggcagg gccgggtaat cgacctgctg
     4021 gtgtcatgcc gcaaaccaag agcattgcgg tcaaatgtac gaatgttgct gcgcaggctt
     4081 atttaacaat gcgtctggaa gccagtgccg tttctggtca ggcgatggtg tcggacaatc
     4141 aggatttagg ttttattgtc gccgatcaga acgatacgcc gatcacgcct aacgatctca
     4201 atagcgttat tcctttccgt ctggatgcag ctgcggcagc caatgtcaca cttcgcgcct
     4261 ggcctatcag tattaccggt caaaaaccga ccgaagggcc gtttagcgcg ctggggtatt
     4321 tacgcgtcga ttatcaatga ggtacggaga atgagaagag tattctttag ctgtttctgc
     4381 gggttactgt ggagttccag tggatgggca gctgaccctt taggaacgat taatatcaat
     4441 ttgcacggta acgttgttga tttctcctgt accgtaaaca cagcggatat tgataagacg
     4501 gtagatttag gcagatggcc tacgacacaa ttactgaacg ctggcgatac cacggcactc
     4561 gtccctttta gcctgcggct ggagggatgt cctccgggtt cagttgcgat tttatttacg
     4621 ggaacgccgg catctgatgc caacctgctg gctctggatg atcccgcaat ggcacaaacc
     4681 gtcgccatcg aattacgtaa tagcgatcgc tcccggctcg cactggggga ggcgagcccg
     4741 actgaggaag tagatgcaaa tggcaatgtc acactaaact tttttgccaa ttatcgagcg
     4801 ttagccagcg gtgttcggcc aggtgtggcg aa
//
LOCUS       DAPGOF010000257         4794 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.261, whole genome shotgun sequence.
ACCESSION   DAPGOF010000257 DAPGOF010000000
VERSION     DAPGOF010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.261"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(48..809)
                     /gene="kduD"
                     /locus_tag="RHV13_004483"
     CDS             complement(48..809)
                     /gene="kduD"
                     /locus_tag="RHV13_004483"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HDV1243923.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTVLGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(839..1675)
                     /gene="kduI"
                     /locus_tag="RHV13_004484"
     CDS             complement(839..1675)
                     /gene="kduI"
                     /locus_tag="RHV13_004484"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HDV1243924.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPVTKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(1962..3143)
                     /locus_tag="RHV13_004485"
     CDS             complement(1962..3143)
                     /locus_tag="RHV13_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDV1243925.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVIDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            3398..4627
                     /locus_tag="RHV13_004486"
     CDS             3398..4627
                     /locus_tag="RHV13_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HDV1243926.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT         1260 a         1224 c         1037 g         1273 t
ORIGIN      
        1 taaaaaaccc tgccatgcgg cagggtcata aaagtaagaa gaatgaatta acgcgccagc
       61 caaccgccat ccacggcaat ggtataacca ttcacataat ctgaagcgct ggaggcaagg
      121 aacactaccg gccccatcag gtcactcggc agtccccaac ggccagctgg aatgcggtcg
      181 agaatttccg cgctacgttg ttcatctgcc cgcaattgtt gagtattgtt ggtcgccatg
      241 taacccggag ctatcgcatt aacattaatg ttgtgtttag cccattcgtt cgccatcaat
      301 cgcgtcacgc ccatcacacc gctttttgat gcggtataag aaggcacacg gatcccgccc
      361 tggaaggaga gcattgacgc gatattgata atcttgccgc cattgccttg cgcgataaag
      421 tgtttcgccg ctgcctgaga catgaagaat acgctcttga tattcaggtt catgacatcg
      481 tcccagtctt tttcgctgaa ctcgagagca tcttcgcggc gaatcaatcc ggcgttattc
      541 accaggatat caatatgacc aaactcagct accgcgcgat ccagcagtgc tggaatgcca
      601 tcaatctttc gcagatcggc ggtcaggctt aaaaaacgac gccccagcac cgtgacctgc
      661 ttgatggttt cagtcggttc aacgatgtta atgccaacaa tgtcacagcc cgcttgcgcc
      721 agccccaacg ccatcccctg gcccagtcca gtatcacaac cagtgacgac cgcaacttta
      781 ccttcgagag aaaatgcact taaaatcata acaatacctt attcgtttat gcccacaact
      841 agcgcaaatc tttaacggcc acatggtcca tatcatcaaa gacctggttt tcaccgacca
      901 tgccccagat aaaggtataa gctttggttc cgacaccgga atgaatcgac cagctcgggg
      961 agatcaccgc ctgctcgtta tgcattacaa tatgacgcgt ttcttgcggc tgccccatca
     1021 tgtggaaaac gcaggcgtca tcatccatat tgaaatagaa ataaacttcc atccggcgct
     1081 cgtgagtgtg acacggcatg gtgttccaca agttacctgg agccagctcc gtcagcccca
     1141 tactcaattg gcaggtttcc agcacatccg ggacaaaata tttgttaatt gtgcgacggt
     1201 tactggtgag gttatcgcct aacgtgactg gagatacttc gtccggtgtg acttttttgg
     1261 tgggatacgt cgtatgcgcg ggtgcgcaat tgtaataaaa ctttgccgga gtggcggtat
     1321 caatactggc aaagacaact tcttttgcac ctttacccac atacagggcg tcgcggtgac
     1381 cgatttcata gcattgacca tcgacagtaa tcgtaccggc accgccaata ttgataacac
     1441 ctaactcgcg acgttcaagg aaataactta cgccgagttg tttaccaact tccccgccaa
     1501 cggaaaccgt tttagttacc ggcataatgc cgccaacaat aattcggtca atgtggctgt
     1561 aaaccatggt gtactcatcg gcgacaaata ccttttcaac caaaaattca ttgcgcagcc
     1621 cctgggtatc cagtgttttt gcgtgcgcac tgtggatgct ctgtcttacg tccacatcaa
     1681 cctccgataa tcagtgaagc gtgaaaatag aaaacgatag tgaaacactg tttcgttttc
     1741 tctataaggg gatgatatgc ccatgggata gggttttcaa ttaaaaataa aacaatgttt
     1801 catttttaag ttaaggatta aaaaaagtgc agtgtcgatc acgaatatgt catgagaatg
     1861 gaaaaatcag ggagtcctga aaaggtatgt cgactcacca gccatcatta ttcaacagat
     1921 taatgacggc agatatttat cggtaaggag gaaatgcaga gctactcgtc acgttcaatg
     1981 gtcaatgcca cgccctggcc cccgccgata caaagcgttg ccagtccttt gcgggcatta
     2041 cgtttcacca tttcatgaac cagagaaacc aggattcggc aaccggaagc gcctatcggg
     2101 tgaccgagtg cgatcgcgcc accattgaca ttgacccgac gctcatccca ttcaagcatc
     2161 ttgccaaccg aaagcgcctg tgcagcaaac gcttcattag cctcgataag atcgacatca
     2221 gccaactgcc agcccacacg ctccaggcaa cggcgggtcg catacaccgg cgcaattccc
     2281 atcaatgctg gatctacacc aacgctggca aatgcgcgga tgcgggccag cacgggtaaa
     2341 ttcaacgctc gtgctttggc ttcgctcatc atcattaccg cagctgcgcc atcgtttatg
     2401 gatgatgcat tacccgctgt cacagaaccg agactatcaa atgaaggatt taaacgggct
     2461 aagccttctg cactggcgtc agtgcgtggc tgttcatcgg tatcaataac caacgtctga
     2521 ccattacttt gggtcattac cgggacgatc tcatctttaa atcgtccagc gtcaatcgcc
     2581 gctcgcgctt tttgttgcga gctaagtgcg taagcatcct gcaactgacg gctgatgcca
     2641 tattcgcgag ccagattttc ggcggtgaca ccaatatgat aatcattgaa ggcatcccac
     2701 aacccatcat gcaccagact gtcaaccaac tggctattgc caagctgtgc accggtgcgg
     2761 ctatcagtca gaacatgtgg tgcgcggctc atgttttcct ggccaccggc gatgacaata
     2821 tcagcctcgc cacactgtat cgcctgagta gccagatgca gtgctttaag cccagaaccg
     2881 caaacgtcat taatcgtgat tgcagaaacg ctattaggaa gaccaccttt aatagccgat
     2941 tgccttgccg gattctgccc tgcccctgca gtcaacacct ggccaagaat cacttcatcc
     3001 accgcatatg caggcacgcc ggtacgttct attaacgctt tcacgaccag actaccaagt
     3061 tccacggcgg aatgacccgc taacgcacca cgaaagcagc cgataggtgt ccgtaacgcc
     3121 ccgacaatca caacgtcttt catcacaacc tcgcaccaat taacagtgca atagtaaatg
     3181 attgttaatt acagttatct taattgttta aaaaaagtga tttttatcac aaaggaaata
     3241 tgcctgagca gcagtcagag acataactgg cacgtaaggt ttgcaaccac taacccacca
     3301 atagaggggt agatagggcg ttaatctccc atacttaacc tggtttatgg taaattgccc
     3361 tccattttgt ttaatttgta gatgatacgt tcagataatg tctaatattt ggtcaaaaga
     3421 agaaactctg tggagtttcg cgctctacgg cacagccgtt ggtgcaggca cgctcttcct
     3481 tcctattcag ttaggttcgg caggggcagt ggtcctgttt attactgctc tggtcgcctg
     3541 gcctttaaca tattggccac ataaagcctt atgccagttc atcctctcat cgaaaacatc
     3601 agcaggtgaa gggataacgg gcgcagtaac acactactat ggcaagaaga ttggtaatct
     3661 gattaccacg ctgtacttca tcgccttttt tgtcgtcgtg ttgatatatg cagtggcaat
     3721 taccaactca cttacggaac agctggcaaa gcatatggtt attgatcttc gcatccgtat
     3781 gttggtgagt ctgggtgttg tattaatcct gaatctcatt tttctgatgg gacgccatgc
     3841 aaccattcgg gtaatgggtt ttttggtatt cccattgatt gcctatttct tatttctttc
     3901 catttacctt gtcggtagtt ggcaacctga tctattaacg acccaggtag agttcaatca
     3961 gaataccctt caccagatat ggatatcgat tcccgtgatg gttttcgcct ttagccatac
     4021 gcccattatt tctacgtttg ccatagacag acgtgaaaaa tatggcgaac acgctatgga
     4081 taaatgcaaa aaaattatga aagtcgctta tctcatcatc tgcataagtg tactgttctt
     4141 tgtctttagc tgcctgcttt ctattccacc ttcgtatatt gaagcggcta aagaagaagg
     4201 ggtcaccatt ttatcggcgc tttctatgct gccgaacgcc ccagcatggt tgtcaatttc
     4261 cgggattatt gtcgcagtag ttgccatgtc gaaatcattc ctgggtacgt actttggcgt
     4321 tattgaaggt gccacagagg tcgtcaaaac aacattacag caggttggtg taaagaaaag
     4381 tcgtgcattt aaccgcgcac tatcaattat gttggtatcg ctgatcacct tcattgtttg
     4441 ttgcattaac ccgaacgcga tttcgatgat ttatgcgatc agcggcccac tcattgccat
     4501 gatacttttc atcatgccta cgctgtcaac gtatctcatc ccggcgctta aaccctggcg
     4561 ttccatcgga aatctgatta cgctgatcgt gggtattctg tgcgtatcgg taatgttctt
     4621 tagctaatat ccattgtgaa tggttcaaaa ttgctacaat tttgaaccat tccatttttc
     4681 ataatgttca tttgttatta ttccgcccta tcacttctct catcatatcc tagaattatc
     4741 agttagttaa acacacaaag agatctgaac atcttaaata agactattta agat
//
LOCUS       DAPGOF010000258         4730 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.262, whole genome shotgun sequence.
ACCESSION   DAPGOF010000258 DAPGOF010000000
VERSION     DAPGOF010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.262"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..1082)
                     /locus_tag="RHV13_004487"
     CDS             complement(<1..1082)
                     /locus_tag="RHV13_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HDV1243927.1"
                     /translation="MKNKDDEKNNDAGNRGIIEVKGKAAPKKILILIILLIAALFVII
                     ILFKVLSREQVVQQTPLEKSDETLVTNTNNGVSLTTMMKNIEEKEKLDAANRRKAQEE
                     QEQKQADNAPSAPASQKADQTAVNVIANGTPQTASGDPNQPQPLPKSVRQLMGDTMVK
                     IDNQEPGEKNQERDDLQGSQYADGKVSPVLNRRYLLSAGTALSCVLKTKIVTSYPGIT
                     MCQLTRDVWSDNGEVLLARKGALLIGEQNKVMTQGVARVFVNWTTLKDENVNVRIGAL
                     GTDSLGASGLPAWVDNHFGQRFGGALLLSLLGDGLDILKNSTQQTGSNSNITYENTSD
                     ATKEMAKTTLDNTINIPPTAYINQGTV"
     gene            complement(1072..1773)
                     /locus_tag="RHV13_004488"
     CDS             complement(1072..1773)
                     /locus_tag="RHV13_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbG/VirB9 family P-type conjugative transfer
                     protein"
                     /protein_id="HDV1243928.1"
                     /translation="MYGTPSERDGRIQTVDYNEQDVFNVRVKAGAQTTIKFGQDETIK
                     DVGIGDPEAWSVSVRDNTLFLRPKAEEPDTNVTVQTNKHIYPLYLISTTKQPTYILRF
                     NYPKPPSATVMTEKPFPCTDGGIINGHYQLKGDKSIFPYQVWDNGEFTCMRWTNKQEI
                     PVLYRVDADGNEHLVNGDRNKNTMVYYDVAENLRLRLGDQVADIRTSSIVNRPWNKKG
                     TSNGKTRVEKFSYEK"
     gene            complement(1839..2573)
                     /locus_tag="RHV13_004489"
     CDS             complement(1839..2573)
                     /locus_tag="RHV13_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HDV1243929.1"
                     /translation="MENTNYYEHSKKEATKKKFEEKNEKKDYFKAIRDFERSEIEIIK
                     KKAKTFTILAIGEFVVICILGFAIASLAPLKTAVPFLVRVDNSTGYTDIAPQLSDAKE
                     SYQDVETKYFLSKYLINYEAYDWQTIQEQADTVKTMSSQKVFSAYDTMIRADSSPLNI
                     LKNNYKIKVQINSVILLRKDMAQVRFKKMVLDLSGKPAPGYRATEWISTISFDWDKDI
                     KTEKERLVNPLGLQVLSYQPDPEVIK"
     gene            complement(2573..2707)
                     /locus_tag="RHV13_004490"
     CDS             complement(2573..2707)
                     /locus_tag="RHV13_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traF protein"
                     /protein_id="HDV1243930.1"
                     /translation="MKIPLIIASALLISACSHTPNPVQKKGGWFELNTAIEQIKAGDY
                     "
     gene            complement(2739..>4730)
                     /locus_tag="RHV13_004491"
     CDS             complement(2739..>4730)
                     /locus_tag="RHV13_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDV1243931.1"
                     /translation="LTFGIPYDDVDTGIERMNEMTQQAQKALQPFNASVLSVHNTYIS
                     EVADYLSLLLNTEHNMIPLSSTPLSSSICDSEWYFGADVLELRNNESDSKKFATNYIL
                     KDFPIETTPGQWDFLLKQPYEFILTQSFIFESPTKTLKNIDSQLNKLQSANDAAKTQQ
                     EELEAGKEAVAAGITLFGSLHCALTVFGDTPDQARSNGIKLSAEFITSGKGFRFSRAS
                     LASPFVFFSHMPLNKRRPLDTRRTITNLACLMSFHNYSSGKKSGNPIGDGSAIMPLKT
                     VSDSIYWFNTHYSPPEKNVTGQKIAGHGMILGATGTGKTTFEAAASGFLQRFDPLMFV
                     VDFNRSTELFVRAYGGSYFTLQEGIYTGCNPWQLAEGPESPVWHRLLAFLKRWTQVLA
                     RDNQGNPCSDEHGIELNAAVESIMRMPVEERRTALLLDIVSPELQTRLAKWCDNGEYA
                     WAVDSPRNTFNPLHHKKVGFDTTVVLDTKNGIHPACEPLLAVLFFYKEIMQRGGNLML
                     SIIEEFWMPANFPMTQSMIKSALKAGRMKGEMMWLTSQSPEDAINCAIFAALVQQTAT
                     KILLPNPDAKWEGYKEIGLTEKEFEKLKELTKESRTMLIKQSGSSVFAKMDLFGFDEF
                     IPVLSGSETGLSIFDEIIAEKGDVAPDIWIPELLKRLNG"
BASE COUNT         1166 a         1016 c         1042 g         1506 t
ORIGIN      
        1 acagtcccct gattaatata tgccgtgggg ggaatattta tcgtattgtc cagcgttgtt
       61 tttgccattt cttttgttgc gtcagatgtg ttttcgtagg tgatattgct gtttgagcct
      121 gtttgttgcg tgctgttttt cagaatatct aaaccatcac caagtaatga cagcagcaat
      181 gcaccaccaa aacgctgacc aaaatgatta tcgacccatg caggaaggcc ggaagcgccc
      241 agactgtcag tgccaagtgc gccaatgcgc acattgacat tttcatcttt gagcgttgtc
      301 cagttaacga acacacgggc tactccctgt gtcatgactt tattctgctc cccgatcagt
      361 aatgctcctt tacgggcaag aagcacttcg ccgttatccg accagacgtc tcgtgttaac
      421 tgacacattg tgataccggg ataagaggtt acgattttcg ttttgagcac acaggataat
      481 gctgttcccg cagataacag atatcggcgg ttcagcacag gggatacctt tccgtctgcg
      541 tactgactgc cctgtaaatc atctctttcc tggttttttt cgcctggttc ctgattgtcg
      601 atcttcacca ttgtgtcgcc catcagttgt cggacggatt ttggcaatgg ttgtggttgg
      661 tttggatctc cactggcggt ttggggagtg ccgttggcta tcacattgac ggctgtttga
      721 tcggcctttt gtgatgctgg cgctgacggg gcattgtctg cctgtttctg ttcttgctcc
      781 tcctgtgctt tcctgcggtt agcggcatcg agtttttcct tttcttcgat gtttttcatc
      841 attgttgtca gtgaaacgcc attgttggta tttgtgacca gtgtctcgtc agacttctca
      901 agaggtgttt gctgaacgac ctgttctcgt gacagaactt taaacaagat aatgatcaca
      961 aataacgcgg caatcagtaa tatgatgagg attaatattt ttttgggcgc tgctttacct
     1021 tttacttcga taattccgcg atttcccgcg tcattatttt tttcatcatc tttatttttc
     1081 atagctgaac ttctctactc gtgtttttcc gtttgacgta ccttttttat tccacggacg
     1141 attaacgatt gatgatgttc tgatatctgc aacctgatca cccaggcgca aacggagatt
     1201 ttcagccacg tcatagtaaa ccattgtgtt tttatttctg tctccgttaa ccagatgttc
     1261 atttccatcg gcatcaacac gataaaggac cggaatttcc tgtttattag tccatctcat
     1321 gcatgtaaat tcgccattat cccatacctg ataagggaaa attgatttat cccccttgag
     1381 ttgataatga ccattaatta ttccaccatc agtgcatgga aatggctttt cagtcattac
     1441 ggtagctgac ggtggtttcg gataattaaa acgcagaata tacgtgggtt gtttcgtggt
     1501 tgatatcagg taaagaggat atatatgctt attggtctga acggtaacgt ttgtatccgg
     1561 ttcttctgct tttggacgca gaaacagagt gttgtccctg accgatactg accaggcttc
     1621 tggatcacca attccgacat cttttattgt ttcgtcttgt ccgaacttaa ttgttgtttg
     1681 tgcgcctgct tttacgcgca cattaaatac atcctgctcg ttataatcta cggtctgaat
     1741 acgcccgtct cgttccgatg gtgtgccgta catagctgct aaagcaccag gtacttgtag
     1801 catcgacaaa agaactatcg cagtagtgcg tttaagcatc attttataac ctctggatca
     1861 ggttggtagc tcagaacttg cagtcccagc ggattcacaa ggcgctcttt ttctgttttg
     1921 atatctttat cccagtcaaa agaaatcgtc gaaatccact ctgttgctct gtatccgggg
     1981 gcgggtttac cagataaatc cagtaccatt tttttgaaac gcacctgcgc catatccttc
     2041 cttagaagga ttactgaatt aatctggacc ttaattttgt agttgttttt cagaatgttc
     2101 agtggtgagg aatcagcacg aatcatggtg tcataagctg aaaacacctt ttgggaagac
     2161 attgttttta ctgtatcggc ctgttcctgg atggtctgcc agtcgtatgc ttcatagtta
     2221 atgagatatt ttgacaggaa atatttagtc tcaacatcct gataactttc cttagcatca
     2281 gataattgag gggcaatatc agtatatcca gtgctgttat ccactcgcac aaggaacggt
     2341 acagcggttt ttaatggagc aagggaggct attgcgaaac caagaatgca aattacaaca
     2401 aattcaccaa tggcgagaat ggtaaatgtt tttgcctttt tctttatgat ttcaatttca
     2461 ctgcgctcaa agtcacgtat ggccttgaaa taatcctttt tttcgttttt ttcttcaaat
     2521 ttctttttgg tggcttcttt ttttgaatgt tcataatagt ttgtgttttc cattaataat
     2581 cacctgcttt gatctgttca attgctgtgt ttaactcaaa ccatccccct tttttctgca
     2641 caggattggg ggtgtgagaa catgcactaa ttaatagcgc ggaggcaatt atgaggggga
     2701 ttttcatatt gttttccgtt ctgtataaaa cagagaaatt aaccgttcag gcgtttcagg
     2761 agttctggta tccagatatc aggagctaca tctccttttt cagcaataat ctcatcaaaa
     2821 atagacaggc cagtttcaga gccagaaagc acggggataa actcatcgaa tccaaacaaa
     2881 tccattttgg cgaaaacaga agagccagat tgcttgatga gcatggtcct tgactccttc
     2941 gtcagctctt tgagtttttc aaactctttt tcagtaagcc caatttcttt atatccttcc
     3001 cattttgcat cgggattggg caggagaatt ttcgttgcgg tttgttgtac cagcgccgca
     3061 aaaattgcgc agtttatggc gtcttccggg gactgtgatg ttaaccacat catttcacct
     3121 ttcatgcgcc ccgctttcag tgcgctttta atcatgcttt gtgtcatggg aaagtttgct
     3181 ggcatccaga actcttcaat aatggagagc atcagattgc cgccgcgttg catgatttct
     3241 ttgtagaaaa acaggaccgc gagtaaaggt tcacaggcag gatgaatacc gttttttgtg
     3301 tccaggacaa cggttgtatc aaatcccact tttttatgat gcagtggatt gaaggtattc
     3361 cggggggaat ccaccgccca ggcatattcg ccgttgtcgc accatttggc gagacgcgtc
     3421 tgaagttcag gactgactat atcgagaaga agggctgtcc ggcgttcttc gacaggcatt
     3481 cgcataatgc tttccacggc agcgtttaat tcaatgccat gttcatcaga acaggggttg
     3541 ccctggttgt ctcgtgccag aacctgtgtc cagcgtttca gaaatgcgag aagtctgtgc
     3601 cagacgggag attccgggcc ttctgccagt tgccacggat tgcagccggt ataaatgcct
     3661 tcctgcaacg tgaaatagct tcctccgtat gcacggacaa aaagctcagt agaacggttg
     3721 aaatccacaa caaacattaa agggtcaaag cgttgaagaa aaccgctggc tgctgcttca
     3781 aatgttgttt tccctgtgcc tgttgcgcca agaatcattc catgcccggc aattttttgt
     3841 ccggtaacat ttttttccgg tgggctgtaa tgcgtattga accagtaaat gctgtcagac
     3901 accgttttca acggcattat ggcggaccca tcaccaatag gattgcctga ttttttcccg
     3961 gaggaataat tgtgaaaaga catcagacag gccaggttgg ttattgttct tcgggtatcc
     4021 agtggacgcc tcttattcag cggcatatga ctgaaaaaaa caaaaggtga tgcgagtgag
     4081 gcgcgactga agcggaatcc cttacccgaa gtaataaact cagcagagag ctttatacca
     4141 ttgcttctgg cctgatctgg tgtatcaccg aataccgtta atgcgcagtg tagtgaacca
     4201 aacaacgtaa ttccggctgc gaccgcttct ttaccagctt cgagttcttc ctgttgtgtt
     4261 ttggctgcat cattagctga ttgcagtttg tttaattgcg aatcaatgtt cttcagggtt
     4321 tttgtgggtg attcaaaaat aaacgattga gtaagaataa attcataggg ttgcttgaga
     4381 agaaaatccc attgtccggg ggtcgtttct atagggaaat ccttcaaaat ataattcgtt
     4441 gcaaactttt tactatccga ttcgttgtta cgcagttcaa gaacatcagc accaaaatac
     4501 cattcagaat cacaaatact ggatgataag ggagtggatg ataaaggaat catgttatgt
     4561 tctgtattta gcaatagtga taaatagtct gccacctcac taatataagt attgtgaact
     4621 gataaaacag aagcgttaaa tggctgaagt gctttttgcg cctgttgtgt catttcattc
     4681 atgcgctcga ttccggtatc aacatcatcg tagggaatcc caaatgttaa
//
LOCUS       DAPGOF010000259         4712 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.263, whole genome shotgun sequence.
ACCESSION   DAPGOF010000259 DAPGOF010000000
VERSION     DAPGOF010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.263"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            159..1262
                     /gene="proB"
                     /locus_tag="RHV13_004492"
     CDS             159..1262
                     /gene="proB"
                     /locus_tag="RHV13_004492"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDV1243932.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1274..2527
                     /gene="proA"
                     /locus_tag="RHV13_004493"
     CDS             1274..2527
                     /gene="proA"
                     /locus_tag="RHV13_004493"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HDV1243933.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQTGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2642..2717
                     /locus_tag="RHV13_004494"
     tRNA            2642..2717
                     /locus_tag="RHV13_004494"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2675..2677,aa:Thr,seq:cgt)
     gene            complement(2844..3743)
                     /locus_tag="RHV13_004495"
     CDS             complement(2844..3743)
                     /locus_tag="RHV13_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV1243934.1"
                     /translation="MKIDLNLLPIFIAVAEESNFSKAAARLGVTRSAVSQGIRRLEDA
                     FGTMLVMRTTRSVNLTEAGERLHKSLSLPLSGIEAAFEEMVSDHVPRGHLRIAVTSIA
                     EEFLSGPLIASFAAANPAVTLDIVVTDEEFDIVAAGYDAGVRLGEVIEKDMIAVPLTG
                     RQREMVVASPSYLAANSTPVHPRELVNHKCIGWRQSPEVAPYRWPFEENGRAFDLAIE
                     PQITTNDLRLMLRLALAGGGITIATQETFRPYIESGKLVSLLDDFLPQFPGFYLYFPQ
                     RRNIAPKLRALIDHVKEWRQQLA"
     gene            3842..4564
                     /locus_tag="RHV13_004496"
     CDS             3842..4564
                     /locus_tag="RHV13_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV1243935.1"
                     /translation="MDKVILITGASSGIGEGIARELGTTGAKVLLGARRVERIEAIAT
                     EICRAGGIAKARELDVTDRQSMADFVQAALDSWGRVDVLINNAGVMPLSPLAAGKQDE
                     WALTIDVNIKGVLWGIGAVLPVMEAQGSGQIINLGSIGALSVVPTGAVYCASKFAVRA
                     ISDGLRQESSKIRVTCVNPGVVESELASTITHVETMKAMDSYRSVALQPADIARAVRH
                     IIESPESVDTTEITIRPTASAN"
BASE COUNT         1188 a         1161 c         1263 g         1100 t
ORIGIN      
        1 attaaatctg taatgaaagc gtaaacaact gccgctacgc ttgctgatcc cgcgcaacaa
       61 aacaccatgc tttgctcgca gatggttggc aaccgacgac agtcctgcta aaacgttcgt
      121 ttgatatcat ttttcctaaa attgaatggc agagaatcat gagtgacagc cagacgctgg
      181 tggtaaaact cggcaccagt gtgctaacag gcggatcgcg ccgcctgaac cgtgcccata
      241 tcgttgaact tgttcgccag tgcgcgcagt tacatgccgc cgggcatcgg attgttattg
      301 tgacgtcggg cgcgatcgcc gccggacgtg agcacctggg ttacccggaa ctgccagcga
      361 ctatcgcctc gaaacaactg ctggcggcgg tagggcagag tcgactgatt caactgtggg
      421 aacagctgtt ttcgatttat ggcattcacg tcgggcaaat gctgctgact cgtgctgata
      481 tggaagaccg tgaacgcttc ctgaacgccc gcgacaccct gcgtgcgttg ctcgataaca
      541 atatcgttcc ggtaatcaat gagaacgatg ctgtcgctac ggcagagatt aaagtcggcg
      601 ataacgacaa cctttctgcg ctggcggcga ttctggcggg tgccgataaa ctgttgttac
      661 tgaccgatca aaaaggtttg tataccgctg acccgcgcag caatccgcag gcagaactga
      721 ttaaagatgt ttacggcatt gatgacgcac tgcgcgcgat tgccggtgac agcgtttcag
      781 gcctcggaac tggcggcatg agtaccaaat tgcaggccgc ggacgtggct tgccgtgcgg
      841 gtatcgacac cattattgcc gcgggcagca agccgggcgt cattggtgat gtgatggaag
      901 gcatttccgt cggtacgctg ttccatgccc aggcgactcc gcttgaaaac cgtaaacgct
      961 ggattttcgg tgcgccgcct gcgggtgaaa tcacggtaga tgaaggggca accgccgcca
     1021 ttcttgaacg cggcagctcc ctgttgccga aaggcattaa aagcgtgact ggcaatttct
     1081 cgcgtggtga agtcatccgc atttgcaacc tcgaaggtcg cgatatcgcc cacggcgtca
     1141 gtcgttacaa cagcgatgca ttacgccgta ttgccgggca ccactcgcaa gaaattgatg
     1201 caatactggg atatgaatac ggcccggttg ccgttcaccg tgatgacatg attacccgtt
     1261 aaggagctgg ccgatgctgg aacaaatggg cattgccgcg aagcaagcct cgtataaatt
     1321 agcgcaactc tccagccgcg aaaaaaatcg tgtgctggaa aaaatcgccg atgaactgga
     1381 agcacaaagc gaaatcatcc tcaacgctaa cgcccaggat gttgctgacg cgcgagccaa
     1441 tggccttagc gaagcgatgc ttgaccgtct ggcactgacg cccgcacggc tgaaaggcat
     1501 tgccgatgat gtgcgccagg tgtgcaacct cgccgatccg gtggggcagg taatcgatgg
     1561 cggcgtactg gacagcggcc tgcgtcttga gcgtcgtcgc gtaccgctgg gggttattgg
     1621 cgtgatttat gaagcgcgcc cgaacgtgac ggttgatgtc gcttcgctgt gcctgaaaac
     1681 aggtaacgca gtgatcctgc gcggcggcaa agaaacgtgt cgcactaacg ctgcaacggt
     1741 ggcggtgatt caggacgccc tgaaatcctg tggcttaccg gcgggtgccg tgcaggcgat
     1801 tgataatcct gaccgtgcgc tggtcagtga aatgctgcgt atggataaat acatcgacat
     1861 gctgatcccg cgcggcgggg ctggtttgca taaactgtgc cgcgagcagt cgacgatccc
     1921 ggtgatcact ggtggtatag gcgtatgcca tatttacgtt gatgaaagtg cagagatcgc
     1981 tgaagcatta aaagtgatcg tcaacgcgaa aacccagcgt ccgagcacat gtaatacggt
     2041 agaaacgttg ctggtgaata aaaacatcgc cgatagcttc ctgcccgcat taagcaaaca
     2101 aatggcggaa agcggcgtga cgttacacgc agatgcagct gcgctggcgc agttgcagac
     2161 aggccctgcg aaggtggtgg cggttaaagc cgaagagtat gacgatgagt ttctgtcatt
     2221 agatttgaac gtcaaaatcg tcagcgatct tgacgatgcc atcgcccata ttcgtgaaca
     2281 cggcacgcaa cactccgatg cgatcctgac ccgcgatatg cgcaacgccc agcgttttgt
     2341 taacgaagtg gattcgtccg ctgtttacgt taacgcctct acgcgtttta ccgacggcgg
     2401 ccagtttgga ctgggcgcgg aagtggcggt aagcacacaa aaactccacg cgcgtggccc
     2461 aatggggctg gaagcactga ccacttacaa gtggatcggc attggtgatt acaccattcg
     2521 tgcgtaaata aaaccgggtg atgcaaaagt agccatttga ttcacaaggc cattgacgca
     2581 tcgcccggtt agttttaacc ttgtccaccg tgattcacgt tcgtgaacat gtcctttcag
     2641 ggccgatata gctcagttgg tagagcagcg cattcgtaat gcgaaggtcg taggttcgac
     2701 tcctattatc ggcaccattt aaatcaagta gttaccccat atttaaatac accacgtttc
     2761 ctcctgtgcc gtatttgtgc cattgtaacc ctggcaatta atcaaaatac tgttctgaca
     2821 tcaggcaatg taggtgcaga catttaagcc aattgctgcc gccattcctt gacgtggtca
     2881 atcagggcgc ggagctttgg tgcaatattg cgacgctgtg ggaaatacag atagaagccc
     2941 ggaaattgtg gaagaaagtc atcaagcaga gatacaagct taccgctttc aatatacggc
     3001 ctgaaagttt cctgagtggc aattgttatt cctccaccgg caagagccag ccgcaacatc
     3061 agacgcagat cattagtcgt aatctgcggt tcaatcgcaa ggtcgaaagc tctcccgttt
     3121 tcttcaaatg gccagcgata aggcgcaacc tccggggact gacgccagcc gatacactta
     3181 tgattcacaa gttcacgcgg atgaacaggt gtgctgttgg ctgcgagata ggagggagag
     3241 gcaacgacca tttcacgctg cctgccggta aggggaacag caatcatgtc tttttcaatg
     3301 acctctccga gccttacgcc agcatcgtaa cctgcggcaa cgatatcgaa ttcctcatcc
     3361 gtgacaacaa tatcaagcgt aacggctgga ttggcagctg caaacgaagc gattagcgga
     3421 cctgaaagaa attcttctgc tatcgacgtc accgcgattc tgagatgtcc acgtggtaca
     3481 tggtcagaga ccatctcttc gaacgctgcc tcaataccgg ataaaggtaa tgacaacgat
     3541 ttatgcagcc gttctcccgc ttccgtcaaa ttaactgagc gcgtggtccg cattacaagc
     3601 attgtcccga atgcatcttc aagacgtctt attccctgac tgaccgctga acgggtaacg
     3661 cccagtcgtg cggcggcttt actaaaatta ctctcttctg ctaccgcgat aaaaatgggt
     3721 aaaagattaa gatcgatttt cattgtttaa tgccgctaac cactgagtac attgaaagca
     3781 ggatactgga accaatcata aagttctatt ctctctccga acacatttga aggagaacgt
     3841 aatggataag gtcatattaa ttaccggcgc atcaagtggt attggggaag gtattgccag
     3901 agagcttgga acgacaggtg caaaggtttt actgggagca cgcagagttg agcgtatcga
     3961 agccattgca accgaaatct gccgcgcagg aggaattgct aaagcgcggg agttggatgt
     4021 cacagaccga cagtccatgg ccgatttcgt gcaagcagcg ctggatagct gggggcgagt
     4081 tgatgttctt atcaataatg cgggcgttat gccgctttca ccgcttgcag caggaaaaca
     4141 agatgaatgg gcgctcacta ttgacgtgaa tatcaaaggt gtactgtggg gaattggcgc
     4201 tgtacttccg gtgatggaag cacagggttc cggtcagata atcaatcttg gttctattgg
     4261 tgccctttct gttgtgccca caggcgcagt ctattgtgca tcaaaatttg cagtacgggc
     4321 tatttctgat ggcttacggc aggaaagctc gaaaatccgt gtgacctgcg taaatcctgg
     4381 cgttgtcgaa agtgaactgg cttcaaccat tacccatgtg gaaaccatga aggcaatgga
     4441 ctcctaccgt tctgttgcac ttcaacctgc ggatattgcc cgtgcggtaa ggcatattat
     4501 tgaatctccc gaaagcgttg atacaaccga aattaccatc agacccactg catctgcaaa
     4561 ctgaagaata acttttacat tcacgtttta ttaaataggt cagaaggaag atttatatat
     4621 ccctgtgttt ttcatagcaa gatgcattat gtattgctca ccccgaagga gatgaaggtt
     4681 taattacaga aagattattt tcacaggaag ta
//
LOCUS       DAPGOF010000260         4617 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.264, whole genome shotgun sequence.
ACCESSION   DAPGOF010000260 DAPGOF010000000
VERSION     DAPGOF010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4617
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.264"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            139..486
                     /locus_tag="RHV13_004497"
     CDS             139..486
                     /locus_tag="RHV13_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243936.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTVLTGGLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLSGLCMASFHWW
                     RRKVNRLPPWDDV"
     gene            complement(506..1096)
                     /locus_tag="RHV13_004498"
     CDS             complement(506..1096)
                     /locus_tag="RHV13_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243937.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDLERVLERSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRLRAEQR
                     AAEVVVLPLVPAAGLPADGTSATDLAVQLNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     RVVRREGDPLEVAQWLQACHDAGMEVRVQINESAAP"
     gene            complement(1096..1353)
                     /locus_tag="RHV13_004499"
     CDS             complement(1096..1353)
                     /locus_tag="RHV13_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243938.1"
                     /translation="MDEERVFSLSYEQLTRFAEKRIRECNLDSQGAIYLCESAKAGAV
                     LIFWHELAINGYASMNAIKRQELIDADFQRLRNLIWPEDDR"
     gene            1921..2334
                     /locus_tag="RHV13_004500"
     CDS             1921..2334
                     /locus_tag="RHV13_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243939.1"
                     /translation="MFFIENEGQAVARTDYWQSVQAQAGYVYLSWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGTLHGRDALELVFEDGSDAPFVIHMLSEQCDRLLPENNQGG
                     GFVVTVWTRGGNQLRYPGKYRVVENLPDVSPWSEH"
     gene            2336..3115
                     /locus_tag="RHV13_004501"
     CDS             2336..3115
                     /locus_tag="RHV13_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HDV1243940.1"
                     /translation="MQHLPAPIHHARDAAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLTPPYPFVQLATLKQR
                     TEKAARTAGRMPAGQQTHRLVPLSDTWYVSQLQTMVATLKIPMERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEVYTKVFALDVAARHRVQFSMPESDAVSMLKRIP"
     gene            3295..3939
                     /locus_tag="RHV13_004502"
     CDS             3295..3939
                     /locus_tag="RHV13_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDV1243941.1"
                     /translation="MPVIANTHPKGGVGKTTSSVNIVGEMKSDTVDLDTHTGLSIILG
                     LRPEGKEISVKVPKTVDELIEIMTPYKNSDKTLLIDCGGFDSDLTRTAIAFADCVIVP
                     SKDSLTERIGLMHFDGVLDEISSIMGTDITAHLYLCKVNPNKKKFPKLDAILPSFKHL
                     KLMKSRISARAEFEDVIETGMGITESVHGRYSAGGKEVIALIEEINHLIENNKQ"
     gene            4026..4334
                     /locus_tag="RHV13_004503"
     CDS             4026..4334
                     /locus_tag="RHV13_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone GroEL"
                     /protein_id="HDV1243942.1"
                     /translation="MSKAKFSAQAEKLKNLVDSARLQTSSSAVTATAPSQPNEGAEEV
                     SGRPVAARTKAKNLKAIPLSYFEAHAELKATSKTSLDFSSYIIEAIREKLERDGAIGQ
                     "
BASE COUNT         1065 a         1144 c         1199 g         1209 t
ORIGIN      
        1 ttctgttcat ctgccagttt tcctttcttt tgcacttttg tcagtgtact gatgcatgac
       61 aacataacca cagcatgata ataataatca ataacaataa gctgtgtcac gtttacatca
      121 tggaacggag gtgctcctgt gacctttgag cagaaaaaag cccgggccat tgccctgatg
      181 gacagcaaaa agatgtggcg gagcaattat gcccctcctc ttctgcgtat tctctggcgg
      241 ctgggtatcc ggcttccgcc attacccttt atgccgttct ggcaggtcac ggtgctgacg
      301 gggggcttat ggggcatttc ctggggatgt gcaatgtggt ttatctactg ggggccgtca
      361 ggaatggtgg caggtgaggc cattattata agtatcacgg ggggtttttt gtccgggctg
      421 tgcatggcct ctttccactg gtggcgcagg aaagttaacc ggctgccacc atgggatgat
      481 gtgtaaaccg tgcacaggcg tgttcttacg gagcggcact ttcatttatc tgtacgcgaa
      541 cctccattcc tgcatcgtga caggcctgca gccactgcgc cacttccagc ggatcgccct
      601 cccggcgtac caccctgcct tctttattcc ataactgcag acaggtgctg ccgtcgagac
      661 gcaccacaaa atccccacgg caggcctgat acgttatgcc gttcagttgc accgccagat
      721 cagtggcaga agtgccatct gcgggcagac ctgctgccgg gaccagcggc agtacgacga
      781 cttctgccgc acgctgttct gcccggaggc ggtcctgttc tgccagcagc agaggctgtg
      841 ccacggcccg gccggcgtcc gtcagctcta ctgccagctg caggttcgga gcgcgcaggg
      901 tacgcagcca gccggcatcc tccagacgac ggcaggaggc gcgcaggttt ggcccgtaga
      961 ccggggcttc cccgcttcgc tccagcaccc gttccaggtc ccgcgtcagc acgggtcctg
     1021 gccgtttttc atcaagggca gccagcacaa tcagcactct ccgctgcagt ggcgacggtc
     1081 ggcggctctg ggtcattacc tgtcatcctc cggccagatg aggtttctga ggcgctgaaa
     1141 gtcggcgtca atgagctcct gtctctttat ggcattcatt gaggcatagc catttatggc
     1201 cagttcatgc cagaagatca gcacggctcc ggctttggct gactcgcaga gataaatcgc
     1261 gccctggctg tccagattgc attcccggat gcgtttttcc gcaaaccggg tcagttgttc
     1321 atagctgagt gaaaacaccc tttcttcatc catcctgcac gctccatctg ataatgttct
     1381 gacaggtaaa aagaatatcc tgcatgattt gtgtgctcct gatttctgat aatatcagat
     1441 atggttttat ttttaatgat gaaaatcata tctgatatga ttttatgtgt attgtttatt
     1501 atcatatcat ttgcgtcttt tctgccgcgc agatccggtt tccggcggtt caggatcttc
     1561 ctctcccccc acacaacgcc acctcctgtc agcacaacat gtggtatctc attcagctgc
     1621 ttatgacact atatgttgtg tcatctccct gacctgtgat gcgccgcgca ggggcggaaa
     1681 acagcgatat gatgattttc tcagcgtggt acacttccgg aaagttgtga tattccggaa
     1741 agtcggatct gacggaaacg gctctccggt aatttaacgg cgtggttata tggatgcttg
     1801 ttatcatggt gatgatgata acggcatgat gttatcagac ggcgtgacgg taagggcagt
     1861 gatgatggat gacgttatcg catgaccgtc cctgcccgga aaagaaaaaa ggagtcaccc
     1921 atgtttttta ttgagaatga aggtcaggct gttgccagaa cggattactg gcagtctgta
     1981 caggcgcagg ccggatatgt ctacctcagc tggaatgccg gcgcagccag gctgcttgtc
     2041 ccggatgcgg caaaacattt actcagggag atgcgggggg ctgagtacgt catcatcagt
     2101 aagggaacgc tgcatggccg cgatgcgctg gaactggtat ttgaagacgg cagcgatgcg
     2161 ccgttcgtga tccacatgct gagtgagcag tgcgatcgcc tgctccccga aaacaaccag
     2221 ggaggggggt ttgttgtcac cgtctggacg cgtggcggta accagctccg ttatccggga
     2281 aagtaccggg ttgtggaaaa cctgcccgac gtttccccgt ggagtgaaca ctgatatgca
     2341 gcacctgcca gcaccgatcc accatgcccg ggatgccgct cagcttcctg ttgccatcga
     2401 ttatccggca gcgctggcac tccgccagat gtcgatggtt catgatgaac tgcccaaata
     2461 cctgctggcc cctgaagtga gcgccctgct ccattacgtc ccggatctgc gccgcaagat
     2521 gctgctggcc acactgtgga acaccggtgc gcgcattaat gaagcactgg cgctgacgcg
     2581 gggggatttt tcgctcacgc ccccgtatcc gtttgtgcag ctggccactc tgaagcagcg
     2641 gacagaaaaa gccgccagga cggcaggaag gatgcccgcc ggtcagcaga ctcaccggct
     2701 ggttccgctt tccgacacct ggtacgtcag ccagctgcag acgatggtgg ccacactgaa
     2761 aatccccatg gaacggcgta ataaacgaac aggcaggaca gagaaagcgc ggatctggga
     2821 agtgacggac agaacggtca ggacctggat tggggaggcg gttgccgccg ctgccgctga
     2881 cggtgtgacg ttctctgtcc cggtcacgcc acatacgttc cgccattcct atgcgatgca
     2941 tatgctgtat gccggtatac cgctgaaggt cctgcagagc ctgatggggc ataagtccat
     3001 cagctcaacg gaggtctaca cgaaggtgtt tgcactggat gtggctgcac ggcaccgggt
     3061 gcagttttcg atgcctgagt ccgatgcggt gtctatgctc aaaagaattc cataagctct
     3121 gactttttta aatccctttc ggatatctca tggatatatt atcggtattt catattctgt
     3181 tgacatgcag gaccggtata gagtaaaaaa acatctattg agtacctctt ggatatccga
     3241 aaggtgtctt atggggtctt tatgggtatt acatgtggat tctggagata cactatgcct
     3301 gttattgcta atacgcatcc caaaggtggt gtgggtaaga caacttcatc cgtaaacatt
     3361 gttggtgaaa tgaagtctga tacagttgat ctggatactc ataccgggct ttctatcatt
     3421 ctggggctga gacctgaagg aaaagaaatt tccgtgaaag tacccaaaac agtggatgag
     3481 ttaatcgaaa ttatgactcc ctacaagaac agcgataaga cgttacttat tgactgtggg
     3541 ggatttgact cagatcttac ccgtactgcg attgcctttg cagactgcgt tattgtccct
     3601 tcaaaagact ccctgactga acgtattggt ttgatgcatt ttgatggagt actggacgaa
     3661 atcagctcta tcatggggac cgatataact gctcacctgt atctctgtaa agttaatcct
     3721 aacaagaaga aattccctaa gcttgatgca atcctgccat cgttcaaaca tctgaagctg
     3781 atgaagagcc gcatttctgc tcgtgcagaa tttgaggatg tcattgagac tgggatggga
     3841 atcactgagt ctgttcatgg gcgctattcg gcaggaggta aggaagttat tgccctgatc
     3901 gaagaaatca atcatctgat tgaaaataac aaacagtaag gtgtgtctat tgggtatcca
     3961 tatagtatcc tttgggcttt taatggatat cttttaagta tctaacaaac atcctgggtt
     4021 atctcatgag caaagcgaaa tttagtgcac aggctgaaaa actgaaaaac ctggtggatt
     4081 ctgccagatt acaaacttct tcttctgccg taacagcaac agccccgtct cagccaaatg
     4141 aaggtgcaga ggaagtttca ggtcgtcccg tggctgccag gacgaaggct aagaatctta
     4201 aggccatacc gctgtcgtat tttgaagctc atgcggaact taaagcgacc agtaaaacat
     4261 ctctggattt tagctcatat attattgagg ctataagaga gaagcttgaa cgtgatggtg
     4321 caataggtca ataaactatc ggatacctta agggtatctg atgtgtgtct tttaaatgcg
     4381 ttatggggat tcttcacgta cctaaagagt ccccattaga catctttctg tttatggtga
     4441 catgattcgt caccgctttc ctttcctctc agtttcacca tttcattcct tttcccttaa
     4501 cctgaaccat gatgtccacg atgtttttat attgcattta tgtgtcacat ataaccttca
     4561 taatgttttt gtatctatga tttcctctgg tcttaaagtt gtgtgtacaa tgtacat
//
LOCUS       DAPGOF010000261         4594 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.265, whole genome shotgun sequence.
ACCESSION   DAPGOF010000261 DAPGOF010000000
VERSION     DAPGOF010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.265"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            137..1321
                     /locus_tag="RHV13_004504"
     CDS             137..1321
                     /locus_tag="RHV13_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HDV1243943.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLADE
                     LKARPIMVGFFFTGSAIMGILVSQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVVTKPVEILPSIHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMIGIAAGLEIPMMLIAGYY
                     MKRIGKRLLMIIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGMIYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIK
                     DI"
     gene            1432..2355
                     /locus_tag="RHV13_004505"
     CDS             1432..2355
                     /locus_tag="RHV13_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDV1243944.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARDE"
     gene            complement(2359..3177)
                     /gene="nlpA"
                     /locus_tag="RHV13_004506"
     CDS             complement(2359..3177)
                     /gene="nlpA"
                     /locus_tag="RHV13_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HDV1243945.1"
                     /translation="MKLTTHHLRAGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSSEVAKAAETIFNGGAVPGW"
     gene            3388..3681
                     /locus_tag="RHV13_004507"
     CDS             3388..3681
                     /locus_tag="RHV13_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243946.1"
                     /translation="MKPTMLLMITVFLTFPAISQAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDESNKQHIREAIVAMERNNQNNYWEALGKVECPDM"
     gene            3983..4480
                     /locus_tag="RHV13_004508"
     CDS             3983..4480
                     /locus_tag="RHV13_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone"
                     /protein_id="HDV1243947.1"
                     /translation="MESANLDLEENKEIASKFERALGMGATLAELHGITPDTLEGVYA
                     YAYNFYEKGRLDEAELFFKFLCIYDFQNYNYLKGYAAVCQLKKDYQKAFDMYHICLML
                     SPDNDFSLVYYMGQCQMGLKNIKMATELFNTVVTYSQNEKVKEMATAYLELLTANTEE
                     EVQTE"
BASE COUNT         1215 a         1028 c         1008 g         1343 t
ORIGIN      
        1 ttgctggccc tataatattg cgctagcatt gttttctggt tgtatcagcg atactcaaaa
       61 attcttaata caatactcat tcgactggta cttatttgta actcagttat attttgtcgc
      121 ccggtgattc agaagaatgc aaaaaacggc taccactcca tcaaaaatac ttgatctcac
      181 tgccgcggca tttttacttg tcgcctttct gacgggtatt gcgggcgctc ttcagactcc
      241 taccctaagt atattcctcg cagatgaact gaaagcccgt cctataatgg taggtttttt
      301 cttcaccggt agcgctatta tgggaattct ggtcagtcaa tttctggcaa ggcactccga
      361 taaacaaggc gaccgtaagt tactgatcct gctatgttgc ttatttggag tgctggcctg
      421 cacgcttttt gcgtggaatc gcaactactt cattctgctc tctacgggcg tacttctgag
      481 tagttttgct tctaccgcaa acccgcaaat gttcgccctc gcccgtgaac acgccgacag
      541 aacaggccgt gagacggtca tgttcagtac atttttacgt gctcagatct cgcttgcctg
      601 ggttatcggg ccaccgctcg cttatgaact ggctatggga tttagtttta aagtgatgta
      661 tctcaccgct gccatcgcat ttgttgtttg cgggctgata gtctggttgt ttttgccatc
      721 aatacaaaga aatattcctg tcgttaccaa acccgtagaa attttaccct ccatccatag
      781 aaagcgggat acgcggctac tttttgtggt gtgttcaatg atgtgggcgg cgaataatct
      841 ctacatgata aatatgccgc tatttattat tgatgaactg catctaaccg ataaactggc
      901 tggagaaatg attggtatcg ctgccggtct ggaaattccg atgatgttaa tcgcaggcta
      961 ttacatgaaa cgtattggca agcgactatt aatgatcatt gctatcgtga gtggtatgtg
     1021 tttttacgcc agcgtactca tggcgacaac tccggcagtt gagctagaat tacaaattct
     1081 aaatgccatc ttccttggta ttctctgtgg tatcggcatg atttattttc aggacctgat
     1141 gcctgaaaaa ataggctctg cgacaacgtt atatgcaaat acttcacgcg tcggctggat
     1201 tatcgccggc tctgttgacg gaattatggt tgaaatctgg agctaccacg cgttgttctg
     1261 gctggcgata gggatgttgg gtattgcgat gatttgcctg ctgtttatta aagatattta
     1321 gctcaccagt ctgggctaag aaaaatcggc gtggcgcaaa cagagccatc ctcgccattt
     1381 ttgctatcat gcctgcatac ataaacgaca aaacagtatg cagagggaaa aatgggttcc
     1441 accagaaagg ggatgctgaa cgttctgatt gccgccgtgt tgtggggaag ttcaggggtc
     1501 tgcgcgcaat acatcatgga gcaaagccag atgtcgtcgc agtttttgac tatgacgcgt
     1561 ttgatattcg ccggtttgat tctgctgacg ctgtcatttg ttcatggcga taaaatcttt
     1621 tctattatta acaatcacaa agatgccatt agtctgctga ttttttccgt agttggcgcg
     1681 ctcaccgtac agctcacttt tttgctaacc atcgaaaaat cgaacgcagc cacggcaacg
     1741 gtgctgcaat tcctctcacc gacgattatc gtcgcctggt tctcactggt acgtaaatcg
     1801 cgcccgggca ttctggtttt ttgcgctatt ttgacatcgc tggtcgggac ttttttattg
     1861 gtgacacacg gtaatccgac gtcattatcg atctctcctg ccgcgttatt ctggggcatt
     1921 gcctcggcat ttgctgctgc attctatacc acctatccct caacgctgat tgcccgctat
     1981 ggcacgttac cagtcgtcgg ctggagtatg ctgattggcg gtctgattct gttgcctttt
     2041 tatgccagac aagggacaaa ctttgtcgtt aacggcagtt tgattctggc gtttttttat
     2101 ttggtggtca ttggtacatc tctgacattt agcctgtacc tgaaaggagc acaattaatt
     2161 ggcggtccaa aagccagcat tttgagctgt gcagaaccat taagtagcgc gctactctct
     2221 ttgctgttgc tggggatcac gttcacatta ccggactggc tgggaacgct gctgattctg
     2281 tcatcggtaa ttttgatttc aatggattcc cgtcgccgcg ccagaaaaat aaatcgtccg
     2341 gcgcgggatg agtgagaatt accagccagg caccgcgcca ccgttaaaga tggtttctgc
     2401 cgctttagcg acttcggatg attgataaga ttgcagaaat tccttcacgt tttctgcatt
     2461 cttattatct tcccgtgcca ccaaaatatt cacatacggc gaatttttat cttcaataaa
     2521 tacgctgtcg tgcaccggag aaagcccggt ctgctgaatg taagtggtgc tgataatcgc
     2581 cacatcaact ttgggatcat ccagtacgcg cgggagctgc gccccctcga gttccataat
     2641 ctgcaaatga cgaggattat cggttatatc cagcgcggta ggtaataacc cttttccctc
     2701 tttcagagta attagcttct ctttttgcaa cagtaaaagc gcgcgaccaa ggttggtcgg
     2761 gtcgttgggg atcgccactg tcgcgccttc ttttatttgc gccacggttt ttattttttt
     2821 ggaataaccc gccatgggga agacaaacgt atttcccacc gctaccagtt tataaccatg
     2881 cgcctgatta tcttgttcaa gaaagggtcg atgctggaag acgttcgcat caagctcgcc
     2941 atgatttgtc gcatcgttcg gtagcaacga accgctaaaa cctaccagtt caacatcgag
     3001 gccatatttc tcttttgcca cctttttagc gacttccgcg acatcttgtt ctgcgccatt
     3061 tataacgcca actttaatgt gttttgcatc gctgctgctc tggtcgcaac ctgccagcag
     3121 aattcctgcc agcaataatg cggcccccgc ccgtagatga tgtgttgtca gtttcacttt
     3181 tttatccttt ttttaatcat taactgacta taatggcaag atcactacga tttaaaaaac
     3241 gaaaagctat cgaatagaag aaaaaggtat aaaaaaactg gcacttctta ctgaccacgc
     3301 aaacaccttt cgtacagcat gatgagagcg gtaaaagcaa actacactta tgcttcattc
     3361 gatgcacatt tgcagaaggt tgccactatg aagccaacga tgctactcat gattaccgta
     3421 ttcctgactt ttccagccat ttctcaggcc gagtcgccat tcagttcgct acagtcggca
     3481 aaagagaaaa ccacggtatt gcaagattta cgtaaaattt gcacgccaca ggcgtcatta
     3541 tcagacgaag cgtgggaaaa attaatgttg tctgatgaga gtaacaaaca acatatccgt
     3601 gaagctattg tggcgatgga gcgtaataat cagaataact attgggaagc gctgggtaag
     3661 gtggaatgcc cagatatgtg aatttcgcac acctccgcgc tcaggccagc agccgctgac
     3721 ttaagctcgc acatcagtaa atcacctgct ttttcaaatt ccaatcaaaa ttgaaccagt
     3781 tcatcaggat tgtggataaa tttccctcta cattattttc atttcatcac atagcaaatc
     3841 ttatctcatt gaaacaatac ttgtttttaa tgcagaaaag gaagcccaac ggatgggcat
     3901 aaaaatgagc gaattttgat atcaccaccc tctaaaaaat ggtgaagtct gaacaaagaa
     3961 tgttcgaaag gagagagtag agatggaaag cgcaaatctg gatttagaag aaaataaaga
     4021 aattgccagt aagttcgaac gtgcactagg tatgggagca acgcttgcag aactgcatgg
     4081 cattactccg gatacccttg aaggcgttta tgcttacgct tataactttt atgaaaaagg
     4141 aagacttgac gaagctgaac tcttttttaa atttctctgt atttatgatt tccaaaatta
     4201 taactactta aaaggatatg ccgccgtttg tcagttgaag aaagattatc aaaaggcatt
     4261 cgacatgtat catatctgtc taatgctatc tcccgataac gatttctctt tggtttatta
     4321 tatgggccaa tgccagatgg gcttaaaaaa tattaaaatg gccacggagt tatttaatac
     4381 agtcgtaacc tacagtcaaa atgaaaaagt taaagagatg gctactgctt atcttgaact
     4441 tttgacagcc aacaccgaag aggaagttca aacagagtga gtcagcaaaa tgaagataaa
     4501 cggaaaaaac gcttctttta cgcctgaaga gctagccaga aaatatcatg aggaagcccc
     4561 ggttgattta aaaaataacc atgtcgataa aata
//
LOCUS       DAPGOF010000262         4557 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.266, whole genome shotgun sequence.
ACCESSION   DAPGOF010000262 DAPGOF010000000
VERSION     DAPGOF010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4557
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.266"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            3464..>4557
                     /locus_tag="RHV13_004509"
     CDS             3464..>4557
                     /locus_tag="RHV13_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDV1243948.1"
                     /translation="MNLLEKIALVGQRMKSEQISLKESLMASSRVSVSDDSVDGVDRL
                     IYNHCLNKKNLSDFFGKSRVTFNKILSDLEEKELVGAPIYQNKNHLYTRWDVQKIMDA
                     LGYPKYRDHYFSRAIVTQNHKGGTGKSTTSVALAVAAALDLQLNARVLMIEWDPQGSI
                     GSSMIQSVSEDDVFLTAIDAILGIYEENSEYKKYLDSGFSEEEIITNMPFSTHLPNLD
                     VITAFPTDARFKDKYWQCSREERTSLLLRFKEVILPVLKQNYDLIIIDTPPEDSPLIW
                     AADEAADGILVAVSPREYDYASTTDFMLTISERFKQSPSKGDNLKWFKVLAVNVDDKS
                     PYERIVLDKLIRTVQDLLMSANIKNSEAFKA"
BASE COUNT         1193 a         1039 c         1038 g         1287 t
ORIGIN      
        1 aacgctttgg tttatgaaaa tttatccaat ttgctcaccg aagactttta acttatttaa
       61 ccccctttat cgccaatctg tgattttcta atgttagcct acagttctag ggcccttgct
      121 cccatgatgc gaatccttcc acctaataaa gattaacgcc tcattcctgt atgccggtct
      181 tacgacgcca gtgcacggac aatttttggt catcacaagg ccaggcggga gatttaatca
      241 gggcgtggtg ttccttagca gcagctgcgg cactgtaagc cgaaggcgga attaactcct
      301 ttatcgccat ctggggacgt cattaggggt acccctttta acttgctcag gaccattaag
      361 ctaaaattcc ggatagtgat tgacgtaaga tttaggctaa gcaggtaaag tgatccccgc
      421 tcccggggtg accactttat acaaaaggcc gatcggaaga ttgtccttag ggaaatattc
      481 ctttaacaca gcacgggcac ttactgccgc tgccggattt aaccccctta atcgccaaac
      541 tggggtactg tcctgatgct ggccttcagt tctgggctgg gtggctatca cttcctccca
      601 taatgcgaaa tgtaactgct gatacagatt aacgtcatat tcctgcgtgc cggtctgcca
      661 acaccagtgc acgggctttt ttcggtcatc acattaccgg gcaggagatt ttatcagggc
      721 gtggtgtgac tgagcagaag ctggggcact gtcagcagct tgcagattta acccccttaa
      781 tcgccacact gggattctgg tctgatattg accttccgtt ctgggcgggg ttcggtttgc
      841 taacacttac tcccatgata cgaaatgtgc ctgctgatac agattaaaat cacattactg
      901 cataccggtc tgccgacacc ggtgcatggg cgatttccga cgatctcaag aacatgtggg
      961 agattatatc agggagagta gtatgcctga gcagcatctg aaggcactgt cagccgctgt
     1021 cagatttaac ccccttaatc gccaaactgg tgttctgtcc tcatgcaggc cttcagttct
     1081 gagctgggtt gctatcactt gctcccataa tgcgaaatgt acctgctgat acaaattaac
     1141 gtcgtattcc tgtatgccgg tctgccgaca ccagtgctcg ggctattttc ggtcctaaca
     1201 agacctggcg agagatttaa tcagggcgtg gtgtgactga gcagcagctg gggcactgtt
     1261 atccgcttgc agatttaacc cccttaatcg ccacactggg gttcagtcct gatagtgtcc
     1321 ttccgtcctg ggctcggttg ctaacacttg ctcccattgc gcctgctgat acagattaaa
     1381 gtcacattac tgcataccga tctgccgact ccggtgcatg gctttttccg ataatcacaa
     1441 ggacggacgg gtgattttat ctttgcatag tatgtctgag cagcatcaga aggctctgtc
     1501 agccactggc agatttaacc cccttaatct ccaaactggg atcagtcctg atagtgtcct
     1561 tccgtcctgg gcagggttgc taacacttgc tcccatgata cgaaatgcgc cagctgatac
     1621 agattaacgt ctcattactc catgccggtc tgccgaaact ggtgcatagc ttttttcgat
     1681 attcgcatga acagacgctt gattttttca ggttgtaata tacctgagca gcatctgaag
     1741 gcactttcag tctctgtcag atttaacccc cttaatcgcc aatctgggat tttctgatgt
     1801 taacctacag ttcaaggccc ttgctcccat gatgcgaacc ttgcaacctg ataaagattt
     1861 aagcctcatt cctgtatgcc ggtcttacga ctctagtgca cggggactat tttcggtcat
     1921 aataagacct ggcgggagat ttaatcaggc gtggtgtgac tgagcagaag ctggggcact
     1981 gtcagccgct ggcagattaa aaccctttat tcgccatctc aagacgtcat tatgggtagt
     2041 ccttttaact ttctaaggac cattaagctt aaactccgtt tagagaatga cgtaagattt
     2101 aggctaagca ggttaagtga tccccgctcc cgggttgaac actttattca tcgtacgagc
     2161 gaaagattgt ccttcgggaa atatttctta tacaccgctc gggcacttac agccactggc
     2221 ggatttaacc cccttaatcg ccaaactggg gtactgtcct gatgctggcc ttcagttctg
     2281 ggctgggtgg ctatcacttg ctcccctaat gcgaaatgtg tctgttgata cagattaacg
     2341 tcattttcct gcgtgccggt ctgccgacac cagtgcacgg ccttttttcg gtcattacat
     2401 taccgggcag gagattttat cagggcgtgg tgttactgag caggagctgg ggcactatca
     2461 gcagcttgca tatttaactc ccttaatcgc caaactggtg ttctgtcctc atgctggcct
     2521 tcagttctgg gctgggttgc tatctcttgc tcccataatg cgaaatgtat ctgctgatac
     2581 agattaacgt catattcctg tatgccggtc tgccgacacc agtgcttggg ctattttcgg
     2641 tcataacaag acctggcgag agatttactc agggcgtggt gtgactgagc agcagctggg
     2701 gcactgtcat ccgcttgcaa atttaacccc cttaatcgcc aaacttgggt tttgtcctga
     2761 tgctggcctt ccgttctggg cggggttgct atcacttgct cccatcatgc gaaaagtgcc
     2821 tgctgataca gattaacgtc atattccttc atgccggtct gccgacacaa atgcacgggc
     2881 ttttttcggt ccttacaata ccgggcagga gatttaatca gggcgtggtg tgactgagca
     2941 gcagctagga cactgtcagc cgctggcaga tttaaccccc ttaatcgcca cactggggtt
     3001 gcctcctgaa gttgggtttc agttctgggc taggttgcta actctgctcc cataattcgt
     3061 agtgggccta ctcaagagat taacacctca tatctttaga ctggactgac gacaacgatt
     3121 cacagttagg aaacgtaaat cagcacaccg gtgagataga ttttagggtc taagcaagaa
     3181 atagggtagg cgtgaagatt cattaatgga gctcataacg tcaaagcgta gagaagtcga
     3241 caagaatcgt ggtttaaaaa gaatgagcat taggtatgga ctagagagtc tgagctctca
     3301 tctgcaagtt aagctcacaa aaaatcataa aaaagtaaac aaacattagc ttaggattga
     3361 aacaaaggat agaattgtac gtataacaat gaaatggacg cattatgcgt aaagtttaca
     3421 tatatgcgta aaagtcggtt ttcagtgcaa aaaggtcata accatgaatt tacttgaaaa
     3481 gatcgccctc gttggtcagc gcatgaaaag cgagcagatt tctcttaagg aatctctgat
     3541 ggcttcatca agagtatctg tttctgatga tagtgttgat ggtgttgata gactgatcta
     3601 caaccactgt ttaaataaaa aaaatctctc tgattttttt gggaagtcac gagtaacgtt
     3661 caataaaata ctttcggact tagaagaaaa agaacttgtt ggtgcaccta tttatcaaaa
     3721 caaaaatcat ctttacaccc gctgggatgt tcaaaaaata atggatgccc tgggttatcc
     3781 caagtaccgc gatcattact ttagcagagc tattgttact cagaatcata aaggcggtac
     3841 agggaaaagc actacatctg tagctttagc agtagcagct gctttagatc tacaactgaa
     3901 tgcacgtgta ttaatgatcg aatgggaccc acaaggttcg attggaagca gcatgattca
     3961 aagtgtctca gaagatgatg tcttccttac agcaattgat gcaatccttg gaatttatga
     4021 agaaaattct gagtataaaa aatatttaga ttcaggattc tctgaagaag aaatcatcac
     4081 taatatgcct ttttcaacgc atctgccaaa cttggacgtg ataacggcat ttccgacaga
     4141 tgctcgtttt aaagataaat actggcaatg ttctagagaa gaacgaacgt ctttgttact
     4201 acgtttcaag gaagttatct taccggtact gaagcagaac tatgatttga ttattataga
     4261 cactccacct gaagattcac ctttgatctg ggctgctgac gaagcggccg atgggattct
     4321 tgtcgcagtg tcaccacgtg aatatgatta cgcctctact acagacttca tgcttacaat
     4381 aagtgaaagg tttaaacaat caccaagtaa gggcgataat ttaaaatggt tcaaagttct
     4441 tgctgtcaat gttgatgaca aaagtccata tgaaagaata gttttggata aattaatcag
     4501 aactgttcag gaccttttga tgtccgctaa cataaaaaat tctgaagcat ttaaggc
//
LOCUS       DAPGOF010000263         4499 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.267, whole genome shotgun sequence.
ACCESSION   DAPGOF010000263 DAPGOF010000000
VERSION     DAPGOF010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.267"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            236..4489
                     /gene="fdeC"
                     /locus_tag="RHV13_004510"
     CDS             236..4489
                     /gene="fdeC"
                     /locus_tag="RHV13_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like adhesin FdeC"
                     /protein_id="HDV1243949.1"
                     /translation="MSRYKTGHKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMGNTTVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDVEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAISAEVTYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLAKQLDTDSIRE
                     RRVLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNTSKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQKPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRSLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     FVPQDTNGVTVGAISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTAKDAYDNPVTSLTPEAPSLAGAAAVGSTASGW
                     TNNGDGTWTAQITLGSTAGELEVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLVAKDAHGNAISGLALSASLTGTASEGATVSSWTEKGNGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKTTTELTFTV
                     KDAYGNPVTGLKPDAPVFSGAASTGSERPSAGNWTEKGNGVYVATLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVNNQLANAQSANQITLTVVDSYGNPL
                     QGQEVTLTLPQGVTSKTGNTVTTNVAGKVDIELMSTVAGEHNISASVNGAQKTVTVKF
                     NADASTGQANLQVDTAVQKVANGKDAFTLTATVEDKNGNPVPGSLVTFNLPRGVKPLT
                     GDNVWVKANDEGKAELQVVSVTAGTYEITASAGNSQPSDTQTITFVADKATATVSGIE
                     VMGNYALADGKAKQTYKVTVTDANNNLVKDSDVTLTASPASLNLEPNGTATTNEQGQA
                     IFTATTTVAATYKLTAQVSQTNGQVSTKTAESKFVADDKNAVLTASSDMQSLVADGKS
                     TAKLEVTLMSANNPVGGNMWVDIQTPEGVTEEDYQFLPSKNDHFVSGKITRTFSTSKP
                     GVYTFTFNALTYGGYEMKPVTVTITAVDANTATGEEAMK"
BASE COUNT         1173 a         1067 c         1272 g          987 t
ORIGIN      
        1 gacttgcaca aaaagctaaa ttttcaccca taaataaaaa tataatcccg cgcccaacca
       61 cctgatgagt ggctacagac actggatata ttaggtggcg gtgcactttc ttacataaag
      121 gtatttcctt ttctgcggaa aaggaaaccg ggaaatcccc ggtttttctg acaagcagac
      181 gccattattt gtgtctgcct atgttcgtta attcgttcat caggaaatta tctcaatgtc
      241 acgttataaa acaggtcata aacaaccacg atttcgttat tcagttctgg cccgctgcgt
      301 ggcgtgggca aatatctctg ttcaggttct ttttccactc gctgtcacct ttaccccagt
      361 aatggcggca cgtgcgcagc atgcggttca gccacggttg agcatgggaa atactacggt
      421 aactgctgat aataacgtgg agaaaaatgt cgcgtcgttt gccgcaaatg ccgggacatt
      481 tttaagcagt cagccagata gcgatgcgac acgtaatttt attaccggaa tggccaccgc
      541 taaagctaac caggaaatac aggagtggct cgggaaatat ggtacagcgc gcgtcaaact
      601 gaatgtcgat aaagatttct cgctgaagga ttcttcgctg gaaatgcttt atccgattta
      661 tgatacgccg acaaatatgt tgttcactca gggggcaata catcgtacag acgatcgtac
      721 tcagtcaaat attggttttg gctggcgtca tttttcagga aatgactgga tggcgggggt
      781 gaacaccttt atcgaccatg atttatcccg tagtcatacc cgcattggtg ttggtgcgga
      841 atactggcgc gattatctga aactgagcgc caatggttat attcgggctt ctggctggaa
      901 aaaatcgccg gatgttgagg attatcagga acgcccggcg aatggctggg atatccgcgc
      961 agagggctat ttacccgcct ggccgcagct tggcgcaagc ctgatgtatg agcagtatta
     1021 tggcgatgaa gtcgggttgt ttggtaaaga taagcgccag aaagacccgc atgcgatttc
     1081 tgccgaggtg acctatacgc cagttcctct tctgacactg agcgccgggc ataagcaggg
     1141 caagagcggt gagaatgaca ctcgctttgg cctggaagtt aactaccgaa ttggcgaacc
     1201 tttggcgaaa caactcgata cggatagcat tcgcgagcgt cgggtactgg caggcagccg
     1261 ctatgacctg gttgagcgta ataacaacat cgttcttgag taccgcaaat ctgaagtgat
     1321 ccgtattgct ctgcctgagc gtattgaagg taagggcggt cagacacttt ccctggggct
     1381 tgtggtcagc aaagcaactc acggactgaa aaatgtgcag tgggaagcgc cgtcattact
     1441 ggctgaaggt ggcaaaatta ccggtcaggg tagtcagtgg caagtaacgc tcccggctta
     1501 tcgtccaggc aaagacaatt attatgcgat ttctgcggtt gcctacgata acaaaggcaa
     1561 tacctcaaaa cgcgtgcaga cagaggtggt cattaccgga gctggtatga gcgccgatcg
     1621 cactgcgtta acgcttgacg gtcagagccg tattcaaatg cttgctaacg gtaatgagca
     1681 aaaaccgctg gtgctgtctc tgcgcgacgc cgagggccag ccagtcacgg gcatgaaaga
     1741 tcagatcaag actgaactaa ctttcaaacc ggctggaaat attgtgactc gttccctgaa
     1801 ggccactaaa tcacaggcaa agccaacact gggtgagttc accgaaactg aagcaggggt
     1861 gtatcagtct gtctttacta ccggaacgca gtcaggtgag gcaacgatta ctgttagcgt
     1921 tgatggcatg agcaaaaccg tcactgcaga actgcgggcc acgatgatgg atgtggcaaa
     1981 ctccaccctg agcgctaacg agccgtcagg tgacgtggtt gctgatggtc agcaagccta
     2041 tacgttgacg ttgactgcgg tggactccga gggtaatccg gtgacgggag aagccagccg
     2101 cttgcgattt gttccgcaag acactaatgg tgtaaccgtt ggtgccattt cggaaataaa
     2161 accaggcgtt tacagcgcca cggtttcttc gacccgtgcc ggaaacgttg ttgtgcgcgc
     2221 cttcagcgag cagtatcagc tgggcacatt acaacaaacg ctgaagtttg ttgccgggcc
     2281 gcttgatgca gcacattcgt ccatcaccct gaatcctgat aaaccggtgg ttggcggtac
     2341 agttacggca atctggacgg caaaagatgc ttatgacaac cctgtgacca gcctcacgcc
     2401 ggaagcgccg tcattagcgg gtgccgctgc tgtaggttct acggcatcgg gctggacaaa
     2461 taatggtgat gggacgtgga ctgcgcaaat tactctcggc tctacggcgg gtgaattaga
     2521 agttatgccg aagctaaatg gacaggatgc ggcagcaaat gcggcaaaag taaccgtggt
     2581 ggctgatgcg ttatcttcaa accagtcgaa agtctctgtc gcagaagatc acgtaaaagc
     2641 cggcgaaagc acaaccgtga cgctggtggc gaaagatgcg catggcaacg ctatcagtgg
     2701 tcttgcgttg tcggcaagtt tgacggggac cgcctctgaa ggggcgaccg tttccagttg
     2761 gaccgaaaaa ggtaacggtt cctatgttgc tacgttgact acaggtggaa agacgggcga
     2821 gcttcgcgtc atgccgctct tcaacggcca gccagcagcc accgaagccg cgcagttgac
     2881 ggtcatcgcc ggagagatgt catcagcgaa ctctacgctt gttgcggaca ataaggctcc
     2941 gaccgtcaaa acgacgacgg aactcacctt caccgtgaag gatgcgtacg ggaacccggt
     3001 caccgggctg aagccagatg caccagtgtt tagcggtgcc gccagcacgg ggagtgagcg
     3061 tccttcagca ggaaactgga cagagaaagg taatggggtc tacgtggcga ccttaacgct
     3121 gggatctgcc gcgggtcagt tgtctgtgat gccgcgagtg aacggccaaa atgccgttgc
     3181 tcagccactg gtgctgaacg ttgcaggtga cgcatctaag gctgagattc gtgatatgac
     3241 agtgaaggtt aataaccaac tggctaatgc acagtctgct aaccagataa ccctgaccgt
     3301 cgtggacagc tatggtaacc cgttgcaggg gcaagaagtt acgctgactt taccgcaggg
     3361 tgtgaccagc aagacgggga atacagtaac aaccaatgtg gcagggaaag tggacattga
     3421 gcttatgtca acggttgcgg gagaacacaa tatttccgct tcggtgaatg gtgctcagaa
     3481 gacggtcacg gtgaaattca acgcggatgc cagcaccggt caggcaaacc tgcaggtaga
     3541 caccgccgtt caaaaagtgg caaacggcaa agatgccttt acgctgacgg cgaccgttga
     3601 ggataaaaat ggtaaccctg ttccagggag cctggtgacc tttaatctgc cccggggtgt
     3661 caagccgctt acaggcgata atgtctgggt gaaagccaac gatgagggga aagccgagtt
     3721 gcaggtggtt tcagtgactg ccggaacgta tgagatcacg gcatcggcag ggaatagcca
     3781 gccttcggat acgcagacta taacgtttgt agccgataag gctaccgcaa ccgtctccgg
     3841 tattgaggtg atgggcaact atgcgctggc ggacggcaaa gccaaacaaa cgtataaagt
     3901 tacggtgact gatgccaata acaatttggt gaaagatagc gacgtgacgc taactgctag
     3961 cccggcttcg ttaaacctgg aaccgaatgg cactgcgaca acgaatgagc aagggcaggc
     4021 tattttcacc gccaccacta ctgttgcggc gacatataaa ctcacggcgc aagtgagtca
     4081 gaccaacggt caggtatcaa cgaaaactgc cgaatctaaa ttcgtcgcgg atgataaaaa
     4141 cgcggtgctc accgcatcat ctgatatgca atctctggtg gcggatggga aatcgactgc
     4201 gaagctggag gtgaccctga tgtcggcaaa taaccccgtt ggcgggaata tgtgggtcga
     4261 cattcagacg ccggaagggg tgacggagga ggattatcag ttcctgccgt cgaaaaatga
     4321 ccatttcgtg agcggaaaaa tcacgcgtac atttagtacc agcaagcctg gtgtctatac
     4381 attcacattt aacgccctga cctatggcgg gtacgaaatg aagccagtga cggtgaccat
     4441 taccgcggtg gatgccaata cggcaacggg cgaggaggcg atgaaataat attaagagt
//
LOCUS       DAPGOF010000264         4310 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.269, whole genome shotgun sequence.
ACCESSION   DAPGOF010000264 DAPGOF010000000
VERSION     DAPGOF010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.269"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..1214)
                     /locus_tag="RHV13_004511"
     CDS             complement(<1..1214)
                     /locus_tag="RHV13_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HDV1243950.1"
                     /translation="MTYASPALRRNPQEVSEHFIKLVHARIAEVSGWKYIFERIPAFK
                     DACAKAPSQVPCPFTGAGKSKFRFRQKDLYTGCAIHNDFPVNEFCDGIDVLAKYYELS
                     KTQTCKKILSDFFGMDLHAPLTDADIENERRYKSAVRATETLDREEVAKRMRKLDVMY
                     HYTGEIKPNTPVALYLRNRGISRLLSHLPKDLGFNNRIYYWDKDKQKSIIYPGMIAIY
                     RDTRGRPLTIHRTYVDKNGDKAPVENPKLMMKPPADMTGGSIQLFDPHYDSGSSTWTL
                     GVAEGIENALSVVEATSTPCWAASSAWCLENVTVPDFLLPPPDVKSINFYIWADKDIA
                     NSQGTRAGIEAAQRLQSRMVEFLAKRYPASKLTIEVFEPAQDIPDGKKGIDWNDVLQL
                     TGQDGFPIHWAP"
     gene            complement(1328..1549)
                     /locus_tag="RHV13_004512"
     CDS             complement(1328..1549)
                     /locus_tag="RHV13_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243951.1"
                     /translation="MTQIKTYRVEHEKVGAMHKVRIFGRVGEVISNDSPQERIFREVT
                     IAEGNSQQAALLVDNYIQRLENNGFTTEA"
     gene            complement(1621..3639)
                     /locus_tag="RHV13_004513"
     CDS             complement(1621..3639)
                     /locus_tag="RHV13_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDV1243952.1"
                     /translation="MKLDLKSSPRHIKRLQNIAKVISGLGDVRVVIDDNTKGPYFDPV
                     NKVCVLPNGDYSDDDFVSLIEGFTCHEAGHGRYTDSEVYSDAFNSVLKSSEGFTRFDD
                     GMNAEFESLAEKRKAYSRAKRLTGLINLFDDVQMEEKVGNDYPDAKRRLAATYALMVK
                     AGRMTPDISSRPENPVLFIEWYLLNSLRVKVLQQAGHKETLDPFFDYAQKILSPVISD
                     VEEIFHDALGCENTQGCESLARKTLALLERLRDEAREQQQQTEQEEQEAYDESETETD
                     TDTDTESEDKSLESTDSREDDSEDDSEGQADTSDNSDLAQEGCSEPSELAEDNEGNNE
                     PTGGNPTGDNIIEDDTDAINPSESESDEASSTNVEDESNFSSAQWEILADLLDAFLGS
                     DEDSEDYHDALAKEIEGIASKVSDDVKAEFGASEWDIPDLLIDLNVYNEALSLSHSIG
                     SDLSVLQQVKMRGRNKTHERGITFDGNRLILSPMGVRDVFRAQSESKNRGHVGLVLVR
                     DISASMANDERYIHAIKSDLALSMAAESLSKMHVSNIVFPFKESEFEIIKTFDQSVEE
                     SLSKFTLGCKGYYTPTGSALMAAVDLLLDSQFDRKIIFLITDGYPNKSEFTIGEVMEK
                     AKCNGIEIVGVGIKTDEIIGFETDTFVTVDDTSLLSIEVSKLVHQILS"
     gene            complement(3717..>4310)
                     /locus_tag="RHV13_004514"
     CDS             complement(3717..>4310)
                     /locus_tag="RHV13_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3150 domain-containing protein"
                     /protein_id="HDV1243953.1"
                     /translation="PISVEGDETQDYYQEQLITDLAEEAMKVYEKISKNNNLRSSTID
                     RLKQMQNKIISFMFIHKEAAVLAEAIKHIMNNLPKGAISEPRDVAVLQQWFYFMSDAS
                     MLKRIISGEQKVTDWLESITRSFNQSSQTEPNALQNTGIEAILDSTNVFQVEPVVEND
                     INNSTFSNTDPVAEPEKSVSQQEDNVENGFDIELKGW"
BASE COUNT         1190 a          998 c          833 g         1289 t
ORIGIN      
        1 tcaggagccc agtgaatcgg gaatccatcc tgccctgtta actggagaac atcgttccag
       61 tcgataccct ttttcccatc aggaatatct tgtgctggtt cgaagacttc aattgtcagc
      121 tttgatgcgg gatatcgttt agccaaaaac tcaaccatgc ggctttgaag tcgctgagca
      181 gcttcaatac cggcacgagt gccttgtgag ttagcaatat ccttatccgc ccagatataa
      241 aagtttatag attttacatc cggcggaggc agtaaaaaat caggaacagt aacgttttca
      301 aggcaccatg cggagctggc tgcccagcat ggtgttgaag tcgcttctac aacagaaagc
      361 gcgttctcga tcccttcagc cactcccagt gtccaggtcg aactacctga atcatagtga
      421 gggtcaaaca actgaattga gccacctgtc atatcggcag gaggcttcat catcagcttt
      481 ggattttcta caggtgcctt atcaccattt ttgtctacgt atgttctatg aatagtcaga
      541 ggccgacctc gggtgtcacg atagatcgca atcattcccg gatagataat cgatttctgc
      601 ttatctttat cccaatagta gatccggtta ttaaagccta aatcctttgg taagtgactc
      661 agtaagcggg agatcccacg attacggagg tacaaagcaa caggagtatt aggcttgatt
      721 tcaccggtat agtgatacat cacatcaagt tttcgcattc gctttgccac ttcctctcgg
      781 tcaagagttt ctgtggcacg aactgctgat ttatagcgtc gttcattttc aatgtcggcg
      841 tcagttaacg gagcatgcag atccatcccg aaaaaatctg agagaatctt tttgcaagtc
      901 tgcgttttac ttaattcata atatttcgcc agaacatcaa ttccgtcgca aaactcattt
      961 accggaaagt cattatggat cgcacatccg gtataaaggt ctttttgtcg gaaacgaaac
     1021 ttcgatttcc ctgcgccagt aaacgggcaa ggaacctggc tcggggcttt tgcacaagcg
     1081 tctttgaaag caggtattct ttcgaatata tacttccagc cagacacttc agcaattcga
     1141 gcatgaacga gtttaataaa gtgttcagat acttcctgtg gattacgacg aagagccggg
     1201 gatgcataag tcatgatgat ctctcttatc tggcattgtg agtttctcaa tgccttctgc
     1261 ggtgcagaag acagggagaa acccacaacg cagaatttaa gaaagagggc ccgggggccc
     1321 tcaacattta tgcttcagtc gtgaagccat tattctcaag acgctgtatg tagttatcta
     1381 caagtaacgc agcctgctgg ctattaccct ccgctatagt gacttcgcgg aagattcttt
     1441 cctgagggct atcattgctt ataacttcac caacacgtcc gaaaatacgg actttatgca
     1501 tcgcaccgac tttttcatgt tctacacggt aagtttttat ctgagtcata ttttgctcct
     1561 tattccttca tggagcaaaa agacccaccg gggccttttg ccccgattgg gtagagttta
     1621 ttaagaaagt atttgatgaa ctagcttact gacttcaata gacaataaag aagtatcatc
     1681 aactgttacg aaagtgtccg tttcaaaacc aattatttca tcagtcttaa taccaacacc
     1741 aacgatttct attccattac atttagcctt ttccataact tcgccgatag taaattccga
     1801 tttattcgga taaccatcag ttataagaaa aattattttc ctatcgaatt ggctgtcaag
     1861 taacagatca acagctgcca ttaaagccga tcccgtaggg gtataataac ccttacatcc
     1921 aagagtaaat ttcgatagag attcctcaac gctttgatca aacgttttga taatctcaaa
     1981 ttcactttcc ttgaagggaa agactatatt ggagacatgc atttttgaca aactttcagc
     2041 ggccatcgat aaagcaagat ctgacttaat ggcatgaata tatctttcat cattagccat
     2101 tgacgctgaa atatcacgaa ctaatacgag gccaacatgc cctctatttt tagattcgct
     2161 ttgagctctg aagacatcac gaactcccat tggtgacaga attaatcgat ttccatcgaa
     2221 ggtaattcca cgctcatgag ttttgtttcg gccacgcatt ttaacctgct gtaaaacaga
     2281 caagtcgcta ccgattgaat gactcagaga caaagcctca ttgtaaacat tcaaatcgat
     2341 aaggagatca gggatatccc attcagaagc accgaattcg gctttcacgt catcggatac
     2401 ttttgatgca ataccttcaa tctctttagc caaagcatcg tgataatctt cgctatcctc
     2461 atcactgcca agaaaggcat ccaacaaatc tgccagtatc tcccattgag ccgatgaaaa
     2521 gttactttca tcttcaacgt tagtcgaact ggcttcatct gattcactct cggaagggtt
     2581 aatcgcatca gtatcgtctt caataatgtt atcacctgtg ggattgccac cggtgggttc
     2641 attatttccc tcgttatctt cagcaagttc tgaaggctca gaacatccct cttgagcaag
     2701 atcagagtta tcggaagtat ctgcctgacc ttcactatcg tcctcagaat catcctcacg
     2761 actatcagtc gactcaagcg atttatcttc tgattcggta tcggtatcgg tatcggtctc
     2821 ggtctcggat tcatcatatg cctcctgctc ctcctgctct gtctgctgct gttgttccct
     2881 tgcctcatcc cgcagtctct ctaaaagagc cagggtctta cgagcaaggg attcacagcc
     2941 ctgagtattt tcacaaccaa gagcatcatg aaaaatttcc tcaacatcag aaattactgg
     3001 agaaagtatc ttttgggcat agtcaaaaaa cggatcgagt gtttctttgt gacctgcctg
     3061 ctgaaggact tttactcgca atgagttaag cagataccac tcaataaata ggacaggatt
     3121 ttccggacga gaagatatat caggagtcat ccttccggct ttaaccatca gtgcgtaagt
     3181 ggctgcaagc cgccgctttg catccggata atcgttacca accttctctt ccatctgtac
     3241 atcatcgaac agatttataa gcccggtaag acgttttgcc ctgctataag ctttacgttt
     3301 ctcagcgagg ctctcaaact ctgcattcat tccgtcatcg aagcgtgtaa acccttcaga
     3361 tgacttgaga acactattaa aggcgtcact gtaaacctcg ctgtcggtat agcgaccatg
     3421 accagcttca tgacaggtaa aaccttcaat cagactgaca aagtcatcat cgctataatc
     3481 gccgtttggt aaaacacaaa ctttatttac aggatcaaaa tacggtcctt tggtattgtc
     3541 gtctattaca actcttacat cgcctaagcc gctaatgact tttgctatat tctgtaaacg
     3601 cttaatatgg cgcggtgatg atttaagatc taatttcata gaatacctct ttataaaaaa
     3661 ggggtactca cccagatggt gaagtaccca tctggggttt ttaataaagc aaacagttac
     3721 cagcctttga gctcgatatc aaaaccattt tcaacattgt cttcctgttg cgaaactgat
     3781 ttctcaggtt cagctacagg atcagtgttt gaaaacgtgc tgttatttat atcgttctct
     3841 acgacaggtt caacctggaa cacatttgtt gaatctaaaa tagcttcaat acctgtgttc
     3901 tgtagcgcgt tcggttctgt ttgtgagctt tgattaaatg atctggtaat agattccaac
     3961 cagtcagtaa ccttttgctc tcctgaaata atacgcttta acattgaagc atcactcatg
     4021 aaataaaacc actgctgtaa caccgcaacg tcgcgaggtt cagaaatggc acctttaggt
     4081 aaattattca ttatgtgttt tatagcttct gcaagtactg ccgcctcttt atgtataaac
     4141 atgaagctaa tgatcttatt ttgcatttgc ttcagtcggt caatagtact tgagcgcaaa
     4201 ttgttatttt tgctaatttt ttcataaacc ttcattgctt cttctgccag atctgttatc
     4261 aattgttcct gatagtagtc ctgggtttca tcaccttcaa cgctaatagg
//
LOCUS       DAPGOF010000265         4233 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.270, whole genome shotgun sequence.
ACCESSION   DAPGOF010000265 DAPGOF010000000
VERSION     DAPGOF010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.270"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            415..678
                     /locus_tag="RHV13_004515"
     CDS             415..678
                     /locus_tag="RHV13_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HDV1243954.1"
                     /translation="MPYHKDRNLYFLSSSMVAQAGQALAWPVSDDAGIPTPVWAITNE
                     RRNSVIAFLVVIGDLCYVTSTDTETPGHHSVGRHVSRAINKPA"
     gene            596..883
                     /locus_tag="RHV13_004516"
     CDS             596..883
                     /locus_tag="RHV13_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraA"
                     /protein_id="HDV1243955.1"
                     /translation="MSPQLIQKLPAITLLEGMFPELSTNQLKVCVFYAMGVPYDAIAQ
                     NCRLSPETVRTYLKRSLKNLNLEGYDALRSAVLMRTFVFMISNTAKENEKM"
     gene            1325..1858
                     /locus_tag="RHV13_004517"
     CDS             1325..1858
                     /locus_tag="RHV13_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusG"
                     /protein_id="HDV1243956.1"
                     /translation="MNIEHLNNRNWYLAQYNTAGKNRESLFSWLNEQNVVPWTPLITR
                     KIRRADSRCCYRERIFAIFPGYFFILANFDIHPVSALRRHSAFIDFVKFGGEIKPVNK
                     DIVDGLMKIYPDPVLNPGAREELNAASSIWLTKAQYQYLLRMENTLQPESRISLLLEL
                     VSNAEHHGFMERLVNIP"
     gene            2112..2795
                     /locus_tag="RHV13_004518"
     CDS             2112..2795
                     /locus_tag="RHV13_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraC"
                     /protein_id="HDV1243957.1"
                     /translation="MTTTEHPVRQLLWCALNALKTAQENHNITSETGLRHYLLEWLSG
                     ASRHPEFRSLPGEIMALKALTEKDRNIPIIGTLNTLFLSSATVGECPLFRFRAALGKL
                     QKAGWRTYVCPWPERVFNESIERACSGKRHLLQLSRTEECFLPTGDMRAPVTLQLITP
                     LHRKTMQLLEAAELIFSDEGFQVVRGYEHQFGIERRETLFHTLFIGLPSLPEDVWGAT
                     QEQTTITLH"
     gene            2809..3375
                     /locus_tag="RHV13_004519"
     CDS             2809..3375
                     /locus_tag="RHV13_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HDV1243958.1"
                     /translation="MKLQYRIPLAIYSVWCIIAILLCKDGLYQMNIWTLVEILGMIAL
                     PLLVRPFFILLRIIFRKNSFKTKDMSDNHTVCVFLSALADTSSKKAIKKHFKQLLEKL
                     PPLLRQKKRIYMKSHLLTEARTQKLICSLRRKGLDVTAERRESAMNSVMFRILIVSRI
                     LSQWKVPHINPRCGIVILTLKNDSGNRE"
     gene            3643..3897
                     /locus_tag="RHV13_004520"
     CDS             3643..3897
                     /locus_tag="RHV13_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HDV1243959.1"
                     /translation="MPQQHPGRLQVLVVDTHCKRKLFSTKTQTDPDELARRFCTPDNC
                     LVVVLCNNRFLFRLERAPGSHCRWRKGSRSRHQHLQDWLS"
     gene            3940..>4233
                     /locus_tag="RHV13_004521"
     CDS             3940..>4233
                     /locus_tag="RHV13_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pili assembly chaperone"
                     /protein_id="HDV1243960.1"
                     /translation="MSFLFKLTIAILIVFAILFPILISSDNKLSALFWKIPKDFWVFL
                     IIMYGAIITVVFIESSEQARIRRQQYLQQYKMPNEIHENVVIKNSIISRETTDD"
BASE COUNT         1169 a         1019 c          933 g         1112 t
ORIGIN      
        1 ctaaataacc tttaaaaaat cattttgtag atcattcgat cgtaaataga atgattctta
       61 gatgataaaa ccatttgtta cacaacccgc caattgtgta acaacagcct ctacaagagc
      121 atatttgttc atatgttcgt cagaactgcg gattgtctgg acgagggtca gacaccgttt
      181 cgtgcatatg gaccagcatg ttcgaacgcc acaaagcaga agcatcatgc ctggatcgct
      241 tcggcgcgga gcgttaagtt ttgacgcttt tttgtatgtt ttttaagctg ttagttgact
      301 cggtgcgtta tactaagtac cctttactgg tcacaggcaa aactgtgatc gggcgtagga
      361 acccgcgtag tcatcgtcgg cgcagaagtt aatttcttca tttggcgtcg ttttttgcca
      421 taccataaag acaggaactt gtatttcttg tcgtcatcta tggtagctca ggcggggcag
      481 gcattagcct ggccggtttc cgatgacgcc ggtattccta cccccgtctg ggctatcacc
      541 aatgagcgta ggaactcggt gatagcgttt ttagttgtca tcggagacct ttgctatgtc
      601 acctcaactg atacagaaac tcccggccat cactctgttg gaaggcatgt ttcccgagct
      661 atcaacaaac cagcttaaag tctgcgtttt ttacgcaatg ggtgttcctt acgacgccat
      721 tgctcagaac tgtcggcttt ctcccgaaac ggtacgaacc tacctgaagc gaagcctgaa
      781 gaatttaaat ctggaaggat atgatgcact tcgctctgct gtgctgatgc gaactttcgt
      841 tttcatgatt agcaatacag ctaaagaaaa cgaaaaaatg tgaacaggtg tccccattaa
      901 aggggacact ttcatggaaa ttgcattttg cactttacag gcgatcccaa attgataaac
      961 ataaacacca agcacaagtt actttaataa agcaaatcac ttcagacggt gtcccagtta
     1021 tttgtatctg gctcatgccc gatatatggc gaccttactc tgaactgacg tgctgatgtt
     1081 ttatttcgcg tatcacaata ctgtgccact gtattgctga tattagtgct tagccgttct
     1141 ttatctgtga gttcgccaaa tcaccgatac agaacgtact agagtctgct ggttatatgc
     1201 ctgtcagaac tgcagtttat ccagatatgg gctggacact gcttcagaca tatgatttag
     1261 catattcgcc tgacatcgca aaatcgtcca tcatggatcg gttttgcgcg gagcgttgaa
     1321 cttcgtgaat atagaacacc tgaataaccg gaactggtat cttgcgcaat ataatacagc
     1381 cggcaaaaac agagaatctt tattctcatg gctgaatgag caaaacgtcg taccatggac
     1441 tccattaata acaagaaaga taagacgggc agactccagg tgctgttacc gtgaaagaat
     1501 attcgctatt tttccagggt acttttttat tctggctaat tttgacattc acccagtatc
     1561 agcattacgt cgacatagcg ccttcattga tttcgtgaag tttggcggtg aaataaaacc
     1621 cgtcaataaa gatattgttg atggattaat gaagatttac ccggaccctg tgctaaatcc
     1681 gggagccagg gaagagctga atgcggcttc cagcatatgg ctgacaaaag cacaatatca
     1741 gtatctcctc agaatggaaa atactctcca gcctgagtcg aggatatccc tgctcctgga
     1801 actggtatca aatgccgaac atcacggctt tatggagcgc cttgtaaaca tcccatagcc
     1861 agtatttccc ggccttcggg ccgggctttt ttcaattcaa gagaatattc cggagtgaag
     1921 cctgcacgaa gccatcccca ccatggactc cccctgtctt ttgatggaac cgtgtatttc
     1981 ttaatggcga aagaaataaa aggcttcacc ccagagtatt gattcgtttt atcaaaggcc
     2041 ggcccgtgcc atcacgtgtg gcggagagaa cggataccgg tcttcttttc atctcacagt
     2101 aggacattcg catgaccacg acagaacatc ccgtgcgcca gttgctctgg tgtgcactga
     2161 atgccctgaa aacggcacag gaaaaccaca atataaccag tgaaaccgga ttacggcatt
     2221 acctgcttga atggctttct ggtgccagca gacatcctga gttccgtagt ctccctggcg
     2281 aaatcatggc gttaaaagca ttgaccgaaa aagatcgcaa tattcccatc atcggaacgc
     2341 tgaatacgct ctttctcagc tctgccactg tcggggaatg tccgttgttt cgttttcggg
     2401 ctgcactggg taaattacag aaagcaggct ggcgcacata tgtctgccca tggcctgaga
     2461 gggtgttcaa tgaatccatt gaacgggcct gttccgggaa acgtcatctg ctacaactaa
     2521 gtcgcaccga agagtgtttt ctaccgacag gcgatatgag ggcaccagtt acgttgcagc
     2581 tgatcactcc actacaccgc aaaactatgc agcttctgga agcggcagag ttaatattct
     2641 ccgacgaagg atttcaggtg gtgagaggat acgaacatca attcgggatc gaacgcagag
     2701 aaaccctctt tcacacctta tttatcgggc tgcccagcct gccagaagat gtctggggtg
     2761 caacacagga gcagacgact atcacattac actgaggaag agcaatacat gaaactccaa
     2821 tatcgtattc cattagctat atatagcgtc tggtgcatta tagcgatttt gctctgcaag
     2881 gatggcttgt accagatgaa catatggacg ctagtagaga tattggggat gatagctctc
     2941 ccgctgttag tccgtccgtt tttcattctg cttagaataa tatttaggaa aaatagtttt
     3001 aaaaccaaag acatgtctga caatcacaca gtatgtgttt tcctgagcgc actcgcagat
     3061 acttcctcaa agaaggcgat aaaaaaacac ttcaagcaat tactcgaaaa gctgcccccc
     3121 ttattacgcc agaagaaacg tatctacatg aaatcccatc ttctgactga agccaggaca
     3181 caaaagctga tttgctctct gcgccgaaaa gggctggatg taacagcgga acgaagggaa
     3241 tccgccatga atagcgtcat gttccgcatc ctcatcgtca gcagaatctt atctcagtgg
     3301 aaagtgcctc acataaatcc ccgctgtggt attgtcattc tgaccctgaa aaacgattct
     3361 ggaaacaggg aataacagtt ataagagcac cacgatttcc gacaggaaaa catacatgaa
     3421 aatcaacgta gatgaccctg gcaaaaagag cttcgctctc attacgcctc acgtgcgtta
     3481 attcccgaca caccccgtga cccgtcttcg cagcctgtac caccctttct gccgttcccg
     3541 ggattctttc tgtcactact cgcttgtgcc gttttttcgc aaccggtacg tgcagttacg
     3601 acggtacagc tgcttctttt cccgttccga aaggagtctt tgatgccaca gcaacatccc
     3661 ggacgactac aggtactggt cgtcgacacg cactgcaaac gcaagctgtt ttcaacgaaa
     3721 acacagacag atcccgatga gcttgcccga cgcttctgca cgccggataa ctgccttgtg
     3781 gtggtactat gcaacaaccg atttctgttc cgcctggaac gtgcacccgg tagccactgc
     3841 cgctggcgca agggtagccg ctcccgccat cagcacctgc aggactggct gtcgtaaaaa
     3901 caacaaaaac aaaagacctc gtcaaaacag gaggggacca tgtcgttttt atttaaatta
     3961 accatcgcga ttttgattgt ctttgcaatc ctttttccga ttttaatttc atcagataac
     4021 aaactgtccg cacttttttg gaagatacca aaagatttct gggttttcct gattatcatg
     4081 tacggggcaa tcatcacggt ggtgttcata gaaagcagtg aacaggcacg catccgccgc
     4141 caacaatatt tgcaacagta caaaatgcca aatgagatac atgaaaatgt ggtgataaaa
     4201 aacagtatta tttcaagaga aacaacagat gac
//
LOCUS       DAPGOF010000266         4135 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.271, whole genome shotgun sequence.
ACCESSION   DAPGOF010000266 DAPGOF010000000
VERSION     DAPGOF010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4135
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.271"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(12..1988)
                     /gene="speA"
                     /locus_tag="RHV13_004522"
     CDS             complement(12..1988)
                     /gene="speA"
                     /locus_tag="RHV13_004522"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HDV1243961.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPSE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(1997..2128)
                     /gene="yqgB"
                     /locus_tag="RHV13_004523"
     CDS             complement(1997..2128)
                     /gene="yqgB"
                     /locus_tag="RHV13_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HDV1243962.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            2264..2479
                     /locus_tag="RHV13_004524"
     CDS             2264..2479
                     /locus_tag="RHV13_004524"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243963.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2783..3937
                     /gene="metK"
                     /locus_tag="RHV13_004525"
     CDS             2783..3937
                     /gene="metK"
                     /locus_tag="RHV13_004525"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDV1243964.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         1057 a         1128 c         1002 g          948 t
ORIGIN      
        1 aataatctta attattcatc ttcaagatag gtataaccgt acaaacctgc ctcgaactct
       61 tcaaggaact gttgttgcag ttcagcatca agatcggttt tcttcacttg atcacggaac
      121 tgagttaaca gcgttttcgg atcgagctgt acatattgca gcatgtccgc cacggtatcg
      181 ccttcgtcag acagttccac ttctacacta ccgtcaggga agacgaacac gtcaaccgct
      241 tcggtatcac cgaacaggtt gtgcatgttg ccgaggatct cctgatatgc gccgaccata
      301 aagaaaccga gcatcggcgg attctctgga tcgtactccg gcataggcat tgtcgtggca
      361 ataccatcac catcgatata gtggtcgata gcaccgtcag agtcacaggt aatatccagc
      421 agcacagcgc ggcgttccgg cacttgatcc agcccttcca gcggcagaac cgggaacaac
      481 tggtcgatcc cccacgcatc cggcatcgac tggaacagcg agaagttgac gtacattttg
      541 tccgccatac gttcctgcag ctcgtcgata atcggacgat gagcacggtt ttgcggatcc
      601 agctgctttt gcacttcatg gcacatgctc aaataaagct gctcagccca tgcacgttct
      661 tgcaggctaa aggtgccgga agagtagccg atatgaatgt cgtgcagatc catctgactg
      721 tcgtgtaacc attcacgcag agaacggcga gttcccggtt cgtgcatctc ctgccaggtt
      781 tcccacatgc tttgcagcgc gcgcggcgca tcttctgaag gcgcggtcgg caccgtgtat
      841 tcgttacgtt ccacgccgat gatattagac accagcacgg tgtgatgcgc agtcactgca
      901 cgacccgatt cggtgattac cgtcggatgc ggcagaccgt tttcttcaca cgcatcacca
      961 atcgcccaga tgatgttgtt ggcatattca ttgaggccgt agtttaccga acagtcggac
     1021 tgcgaacgag taccttcata atccacgccc agaccgccgc cgacgtcgaa gcactgaata
     1081 ttgacgccca gcttgtgcag ttccacatag aaacgcgccg attcacgaac gcctgtggcg
     1141 atatcgcgaa tattcgccat ctgcgaaccg aggtggaagt gcagtagttg caggctgtcg
     1201 agacgcccgg cttcacgcag ggtttcaacc agttgcagta cctgagtcgc agccaggccg
     1261 aacttcgatt tttccccgcc ggaggactgc catttacccg agccctgcga agccagacgt
     1321 gcacgcacgc ccagacgagg aacgacattc agacgttctg cttcatccag cacaatggcg
     1381 atttctgaca tcttctcaat gaccagatag accttgtgcc ccatcttctc gccaattaat
     1441 gccaggcgga tatattcgcg gtctttataa ccgttgcaaa cgatgacgct acgggtcatg
     1501 ccagcatgtg ccagcactgc catcaactcg gctttggaac cggcttccag acccagcggt
     1561 tcgcccgaat gaatcaggga ctcaatcacg cgacggtgct ggttaacttt gatcggataa
     1621 acaaggaagt aatcgccgtt atagccgtag gattcccgcg cacgtttaaa cgcggcgtta
     1681 atggaacgca aacggtgctg caggatctgt gggaaacaga acagtgcagg cagacgctga
     1741 ccctgtgctt cacgagtttt cactaactgc gcgagatcga cgcgagcttc cgggacgtcc
     1801 gggtccgggc acacgctgat gtggcccagc tcgttaacgt catagtagtt attgccccac
     1861 caggcaatat tgtaagtacg cagcatcttg ctggcttcct gggagctcat tgcaacctcc
     1921 tgcatggagc gtagtacacc gtgttcgccc gctgacgaag gcaaacccat agacatgtcg
     1981 tcagacatag cgaacctcaa attattttat taagtgtaaa acagttaacg actatcgcag
     2041 cctggaactg cgataacaac ccataagcac atggattttc cagcagtgaa tgctgacgct
     2101 ccaactgcgc aaccggtttc tttttcataa cattattaag cacataaccg aacgtaagtg
     2161 tgacagttcg gcgaaaccac gagaaaactc ttgtttttac aagagcgccc ttgttcagtc
     2221 ctcagtaact gtaaccagct cttgaatcct gagaagcgcc gagatgggta taacatcggc
     2281 aggtatgcaa agcagagatg cagagtgcgg ggaacgaatc ttcaccagaa cggtgagaca
     2341 ggttaagcag cagacaacgg ttcattattt cgtatcacct ccacggccgc ctgttaagac
     2401 gaacccacaa gccaaaactc tgatttcaac ccggctggaa gtggcaacac gaaagaaacg
     2461 ccgtgtgctt tttatttaag ccgcgcgccg cgttttatac cccacaatgg cagaaaattg
     2521 caaaagataa atacgcagaa tgccggcatt gtcaggaaaa atttccagcc acgtttttaa
     2581 cagaatgaga cacgattcaa aaaaaagtgg aaatcgggtg aagaattgac ctaaaatagc
     2641 catccagatg ttaatccatc cataccgatt aacactcaga ctgccagtgt ttttaacctg
     2701 cagagtcgtg gtaggatccg ctaccacaga aaatccacac aacagtttga gctaaccaaa
     2761 ttctctttag gtgatattaa atatggcaaa acaccttttt acgtccgagt ccgtctctga
     2821 agggcatcct gacaaaattg ctgaccaaat ctctgatgcc gttttagacg cgatcctcga
     2881 acaggatccg aaagcacgcg ttgcttgcga aacctacgta aaaaccggca tggttttagt
     2941 tggcggcgaa atcaccacca gcgcctgggt agacatcgaa gagatcaccc gtaacaccgt
     3001 tcgcgaaatt ggctatgtgc attccgacat gggctttgac gctaactcct gtgcggttct
     3061 gagcgctatc ggcaaacagt ctcctgacat caaccagggc gttgaccgtg ccgatccgct
     3121 ggaacagggc gcgggtgacc agggtctgat gtttggctac gcaactaatg aaaccgacgt
     3181 gctgatgcca gcacctatca cctatgcaca ccgtctggta cagcgtcagg ctgaagtgcg
     3241 taaaaacggc actctgccgt ggctgcgccc ggacgcgaaa agccaggtga ctttccagta
     3301 tgacgacggc aaaatcgttg gtatcgatgc tgtcgtgctt tccactcagc actctgaaga
     3361 gatcgaccag aaatcgctgc aagaagcggt aatggaagag atcatcaagc cgattctgcc
     3421 cgctgaatgg ctgacttctg ccaccaaatt cttcatcaac ccgaccggtc gttttgttat
     3481 cggtggcccg atgggtgact gcggtctgac tggtcgtaaa attatcgttg atacctacgg
     3541 cggcatggca cgtcacggtg gcggagcatt ctctggtaaa gatccatcaa aagtggaccg
     3601 ttccgcagcc tacgcagcac gttatgtcgc gaaaaacatc gttgctgctg gcctggccga
     3661 tcgttgtgaa attcaggttt cctacgcaat cggcgtggct gaaccgacct ccatcatggt
     3721 agaaactttc ggtactgaga aagtgccttc tgaacaactg actctgctgg tacgtgagtt
     3781 cttcgacctg cgcccatacg gtctgattca gatgctggat ctgctgcacc cgatctacaa
     3841 agaaaccgca gcatacggtc actttggtcg tgaacatttc ccgtgggaaa aaaccgacaa
     3901 agcgcagctg ctgcgcgatg ctgccggtct gaagtaatct ttcttcacct gctttcaaag
     3961 gccagcctcg cgctggcctt tttcttttac ctcaccgcgg tggataggcg ttcacgccgc
     4021 atccggcaaa aaaaccgcct gcacaataac atcattcttc ctgatcacgt ttcaccgcaa
     4081 attatcatca caactgaaac cgattacacc aaccacaaca gacaaagatt tgtaa
//
LOCUS       DAPGOF010000267         4128 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.272, whole genome shotgun sequence.
ACCESSION   DAPGOF010000267 DAPGOF010000000
VERSION     DAPGOF010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.272"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            9..3782
                     /locus_tag="RHV13_004526"
                     /pseudo
     CDS             9..3782
                     /locus_tag="RHV13_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT          924 a         1121 c         1390 g          693 t
ORIGIN      
        1 cactttatat gagcggaaaa ccagcggcgc gtcagggaga tatgactcag tatggcggtc
       61 ccattgtcca ggggtcggca ggtgtaagaa ttggcgcgcc aactggcgtg gcgtgctcgg
      121 tgtgtccggg cgggatgact tcgggcaacc cggtaaatcc gctgctgggg gcgaaggtgc
      181 tgcccggcga gacggacctt gcgctgcacg gcccgctgcc gttcattctc tcccgcacct
      241 acagcagcta ccggacccgg acgcctgcac cggtgggctt tttcggcccc ggctggaaag
      301 cgccttctga tatccgctta cagctacgtg atgacggact gatactcaac gacaacggcg
      361 ggcggagcat tcactttgag ccgctgctgc cgggtgaggc ggtgtacagc cgcagcgagt
      421 caatgtggct ggtgcgcggt ggtaaggcag cacagccgga cggccacacg ctggcgcggc
      481 tgtggggggc gctgccgccg gatatccggt taagtccgca tctttacctg gcgaccaaca
      541 gcgcacaggg gccgtggtgg atactggggt ggtctgagcg ggtgccgggt gctgaggacg
      601 tactgccagc gccgctgccg ccgtaccggg tgcttaccgg gatggcggac cgcttcgggc
      661 ggacgctgac gtaccggcgt gaggccgcag gcgacctggc cggggaaatc accggcgtga
      721 cggacggtgc cgggcgggag ttccgtctgg tgctgaccac gcaggcgcag cgtgcggaag
      781 aggcccgcac ctcttcgcta tcttcttctg acagttcccg ccctctctca gcctcagcgt
      841 tccccgacac actgcccggt accgaatacg gccccgacag gggtatccgc ctttcggcgg
      901 tgtggctgat gcacgacccg gcatacccgg agagcctgcc cgctgcgcca ctggtgcggt
      961 acacgtatac ggaagccggt gaactgctgg cggtatatga ccgcagcaat acgcaggtgc
     1021 gcgctttcac gtatgacgcg cagcacccgg gccggatggt ggcgcaccgt tatgcgggaa
     1081 ggccggagat gcgctaccgc tacgacgata cggggcgggt ggtggagcaa ctgaacccgg
     1141 cagggttaag ttaccgctat ctttatgagc aggaccgcat caccgtcacc gacagcctga
     1201 accggcgtga ggtgctgcat acagaaggtg aaggcgggct gaaacgggtg gtgaaaaaag
     1261 aactggcgga cggcagcgtc acgcacagcg ggtatgacgc ggcaggaagg ctcacggcgc
     1321 agacggacgc ggcgggacgg cggacagagt acggtctgaa tgtggtgtcc ggcgatatca
     1381 cggacatcac cacaccggac gggcgggaga cgaaatttta ctataacgac gggaaccagc
     1441 tgacggcggt ggtgtccccg gacgggctgg agagccgccg ggcatatgat gaaccgggca
     1501 ggctggtatc ggagacatcg cgcagcgggg acgtcatccg gtatgcttat gataatccgc
     1561 acagtgaatt accggccacg acaacagatg cgacgggcag cacccggcag atgacgtgga
     1621 gccgctacgg gcagttgctg gcgttcaccg actgctcggg ctaccagacc cgctatgaat
     1681 acgaccgctt cggccagatg acggcggtcc accgcgagga aggtatcagc cgttaccgcc
     1741 gctatgacaa ccgtggccgg ttaacctcgg tgaaagacgc acagggccgt gaaacgcagt
     1801 atgagtacaa cgccgcaggc gacctgactg ccgttatcac tccggacggc aaccggagcg
     1861 agacacagta cgatgcgtgg ggaaaggcgg tcagcaccac gcagggcggg ctgacgcgca
     1921 gtatggagta cgatgttgcc ggacgtgtca tcagtctgac taacgagaac ggcagccaca
     1981 gtgatttcag ttacgatgcg ctggaccgcc tggtacagca gggcggcttt gacgggcgga
     2041 cgcagcgtta tcattatgac ctgacaggaa aactcacaca gagtgaggat gagggacttg
     2101 tcaccctctg gtactacgat gaatcggacc gtatcactca ccgcacggtg aacggcgaac
     2161 cggcagagca gtggcagtat gatggccacg gctggctgac agacatcagc cacctgagcg
     2221 aaggccaccg tgttgccgtc cactatggct atgacgataa aggccgcctg accggcgaac
     2281 gccagacggt ggagaacccg gagacggggg aactgctgtg gcagcatgag acgaaacacg
     2341 catacaacga gcaggggctg gcaaaccgcg tcacgccgga cagcctgccg ccggtggagt
     2401 ggctgacgta tggcagcggt tacctggcgg gaatgaagct gggcgggacg ccgctggtcg
     2461 agtatacgcg ggacaggctg caccgtgaga cggtgcgcag cttcggcagc atggcaggca
     2521 gtaatgccgc atacgaactg accagcacat acacccccgc aggccagtta cagagccagc
     2581 acctgaacag cctggtatat gaccgtgact acgggtggag tgacaacggc aacctggtgc
     2641 gcatcagcgg cccgcgacag acgcgggaat acggctacag cgccacgggc aggctggaga
     2701 gtgtgcgcac cctcgcacca gacctggaca tccgcatccc gtatgccacg gacccggcgg
     2761 gcaaccggct gccggacccg gagctgcacc cggacagtac actcacagtg tggccggata
     2821 accgcatcgc ggaggatgcg cactatgtct accgccacga tgaatacggc aggctgacgg
     2881 agaagacgga ccgcatcccg acgggtgtga tacggacgga cgacgagcgg acccaccact
     2941 accactacga cagccagcac cggctggtgt tttacacgcg gatacagcat gaggagccac
     3001 tggtcgagag ccgctacctc tacgacccgc tgggccgccg gacggggaaa cgggtgtggc
     3061 gtcgcgagag tgacctgacg ggatggatgt cgctgtcgcg taaaccggag gtgacgtggt
     3121 acggatggga cggcgactgg ctgacgacgg tacagaccga caccacgcgt atccagacgg
     3181 tataccagcc ggggagcttc gcgccgctca tccgcatcga gacagagaac ggcgaactgg
     3241 ataaagcgca gcggcgaagc ctggtggaga agttacagca ggaagggagt gaggacggtc
     3301 acggtgtggt gttcccggct gaactggtgg ggctgctgga caggctggag ggggaaatcc
     3361 gggcaaactg cgtgagcagt gaaagccgcc agtggcttgc gcagtgcggg ctgacggtgg
     3421 agcggctggc ggcacagata gagccggttt atttgcctga gcgaaaaata catctttatc
     3481 actgtgacca ccggggcctg ccgctggcgc ttatcagtga agacggcaat acggcgtgga
     3541 gcgcggaata tgatgaatgg ggcaaccagc ttaatgagga gaacccgcat cacctgcacc
     3601 agccgtaccg gctgccgggg cagcagtatg atacggagtc ggggctgtac tacaaccgga
     3661 accggtacta cgatccgttg caggggcggt atatcactca ggacccgata gggctggagg
     3721 ggggatggag tctgtatgcg tatccgctga atccggtgaa tggtattgat ccattagggt
     3781 taagtcccgc agatgtagcg ctaataagaa gaaaagatca actaaaccat caaagagcat
     3841 gggatatatt atctgatact tatgaagata tgaagagatt aaatttaggt gggactgatc
     3901 aatttttcca ttgtatggca ttttgtcgag tgtctaaatt aaatgacgct ggtgttagcc
     3961 gatcggcgaa agggctgggt tatgaaaaag agattagaga ttacgggtta aatctgttcg
     4021 gtatgtacgg cagaaaagta aagctatccc attctgaaat gattgaagat aataaaaaag
     4081 acttggctgt aaatgaccat gggttgacat gtccatcaac aacagatt
//
LOCUS       DAPGOF010000268         4095 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.273, whole genome shotgun sequence.
ACCESSION   DAPGOF010000268 DAPGOF010000000
VERSION     DAPGOF010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4095
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.273"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            186..794
                     /locus_tag="RHV13_004527"
     CDS             186..794
                     /locus_tag="RHV13_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HDV1243965.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            892..2283
                     /gene="selA"
                     /locus_tag="RHV13_004528"
     CDS             892..2283
                     /gene="selA"
                     /locus_tag="RHV13_004528"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HDV1243966.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2280..>4095
                     /gene="selB"
                     /locus_tag="RHV13_004529"
     CDS             2280..>4095
                     /gene="selB"
                     /locus_tag="RHV13_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HDV1243967.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARTQSDADALTVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAELLFGNEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLL"
BASE COUNT          927 a         1031 c         1206 g          931 t
ORIGIN      
        1 aaaaccctac agatttataa gaaagcctta agtgagatat ccatcagata gaaacaatta
       61 ggaaaatatt tatatcacca tgataaatat gtgatttatt tggaagtaac aaaccatcaa
      121 cattttctgc cctttttccc atcaccaggc catactgata tttttcctcg cacaaaggac
      181 tcggcatgaa actcgtcggt agctacacca gcccgtttgt acgcaaactt tctattctgt
      241 tgttagaaaa gggcataact ttcgaattta ttaatgaact gccctataac gcggacaacg
      301 gcgtggcgca atttaacccg ttaggaaaag tgccggtgct ggtgaccgaa gagggcgaat
      361 gttggtttga ttcgccgatc atcgctgaat atattgaatt aatgaatgtc gctccggcga
      421 tgttgccgcg cgatccgctg gagtcgttgc gggtgcgcaa aattgaggca ctggcggatg
      481 gcattatgga tgccgggctg gtatcggtgc gtgaacaggc gcgtccagcg gcgcagcagt
      541 ctgaagatga attgttacgc cagcgggaga aaatcaaccg cagtctggat gtgctggaag
      601 gatatctggt cgatggcaca ctcaaaaccg atacggtcaa tctggcgact atcgccattg
      661 cctgtgccgt cggatatctc aatttccgcc gcgttgcgcc aggttggtgt gtcgaccgcc
      721 cacacttagt caaactggtc gaaaacctgt ttagccgcga aagttttgct cgcaccgaac
      781 cgccaaaggc ttgatgcgtg atatgtcctc ctgacccatc tcacgttaca atccgtggtt
      841 atgttaaacg cccttctccg tgtgagaggg ccttgatcag ccaggtttcc tatgacaacc
      901 gaaacgcgtt ccctctatag tcaacttccg gctattgatc gcttattgcg cgatagctcc
      961 ttcctttctt tgcgtgatac ttatggtcac acccgcgtgg tggaattgtt gcgtcagatg
     1021 ctcgacgaag cgcgagaagt gattcgtggc agccagacgc tgcctgcgtg gtgtgaaaac
     1081 tgggcgcaag aagtcgatgc ccggttgacg aaagaggcgc agagcgcgct gcgtccggtg
     1141 atcaacctga cgggaaccgt gctgcatacc aaccttggac gagctttaca ggcggaagcc
     1201 gcggtggaag ccgttgcgca ggctatgcgt tcgccagtga ccctcgagta cgatctggac
     1261 gacgccgggc gcggacatcg cgatcgggcg ctggcgcagc tgctgtgccg cattaccggc
     1321 gcggaagatg cctgtatcgt taataacaat gcggcggcgg tgttattgat gttggcggcc
     1381 actgccagcg ggaaagaggt ggtggtttct cgcggcgaat tggtggagat tggcggcgcg
     1441 tttcgtattc ccgatgttat gcgtcaggca ggctgcaccc tacacgaagt agggaccacc
     1501 aaccgcacgc acgcgaatga ttatcgtcag gcggtgaatg aaaataccgc actgttgatg
     1561 aaagtacata ccagtaacta cagcattcag gggttcacca aagcgataga cgaagctgaa
     1621 ctggtggcgc tcggcaaaga gctggatgtt cccgtagtga ctgatttagg cagtggctcg
     1681 ctggtcgatc ttagccagta cggtttgccg aaagagccaa tgccgcagga gttgattgcg
     1741 gcgggcgtca gtctggtgag cttctccggc gacaagttgt taggcgggcc gcaggcagga
     1801 attattgttg gtaaaaaaga gatgatcgcc cgcctgcaaa gccacccgct gaagcgtgca
     1861 ttacgcgcgg ataaaatgac cctcgcggcg ttggaagcca cgttgcgtct ttatttacac
     1921 cctgaagctc tgagtgaaaa attaccgacc ctgcgcctgc ttacccgcag cgcagaggtc
     1981 attcaaatcc aggcacaacg tttacaggct ccccttgccg cacattacgg cgcggagttt
     2041 gcggtacagg ttatgccatg tctttcgcag attggcagtg gttcgctgcc ggttgatcgc
     2101 ctgccgagcg cggcattaac gtttacaccc catgatggac gcggtagcca ccttgagtca
     2161 ttagccgccc gctggcgtga attgccagtg ccggtgattg gtcgtattta tgacggacga
     2221 ttgtggctgg atttacgctg ccttgaagat gagcaacggt ttttggagat gttgttgaaa
     2281 tgattattgc gactgccgga cacgttgacc acggcaaaac aaccttattg caggcgatta
     2341 ctggcgtaaa tgccgatcgt ctgccggaag aaaaaaagcg cggcatgact atcgatctcg
     2401 gctatgccta ctggccgcag ccggatggtc gtgtgcctgg ttttatcgac gtacccggtc
     2461 atgagaagtt tctttccaac atgctggcgg gcgttggtgg tatcgatcac gcgctgttgg
     2521 tggtggcgtg cgatgacggt gtgatggcac agacccgcga gcatctggcg attttgcagc
     2581 tgaccggtaa cccgatgctg acagtggcgc tgaccaaagc cgatcgcgtg gacgaagcgc
     2641 gtgttgatga ggttgaacgt caggtaaagg aggtgctgcg ggaatacggt tttgctgagg
     2701 caaaactgtt tatcaccgca gcaaccgaag gtcggggaat tgatgccctg cgcgagcatc
     2761 tgcttcagtt accagaacgt gaacacgcca gccaacatag tttccgcctc gcgatcgacc
     2821 gcgcatttac cgtaaaaggt gccgggctgg tcgtcaccgg tacggcgtta agcggggaag
     2881 tgaaggtagg cgattcacta tggctgactg gtgtaaataa accgatgcgt gtacgtgcac
     2941 tgcatgcaca aaaccagcca acagaaaccg ctcatgccgg gcagcgtatc gcgcttaaca
     3001 tcgcggggga tgcggaaaaa gagcagatta accgtggcga ctggctgctt gccgatgtgc
     3061 cgccagagcc gttcacgcgg gtgattgtcg agcttcaaac ccatacgccg ctgacccagt
     3121 ggcagccgct gcatattcac cacgccgcca gccacgttac gggacgcgtt tcgctgctgg
     3181 aagataacct tgccgagctg gtgttcgaca cgccgctgtg gctggcggat aacgatcgct
     3241 tagtgttgcg cgatatctcg gcccgcaata cgctggcggg ggcgcgtgtg gtgatgctta
     3301 acccgccgcg tcgcggtaaa cgtaagccgg aatatctgca atggctggcg tcactggctc
     3361 gcacacaaag cgatgccgac gcactgaccg ttcatctgga gcgcggtgcg gttaaccttg
     3421 ccgatttcgc ctgggcgcgt cagctcaatg gtgaagggat gcgcgaactg ctgcaacagc
     3481 cgggctatat tcaggctggt tacagtttgt tgaacgctcc agttgccgcc cgctggcaac
     3541 gtaaaattct cgacacatta gcaacttatc atgagcaaca tcgcgatgaa cctggtccgg
     3601 gacgtgagcg tctgcggcgc atggcattgc cgatggaaga tgaagcactg gtgctgttgc
     3661 tgattgaaaa gatgcgcgaa agcggcgaca tccttagtca tcacggttgg ctgcatctgc
     3721 cggatcataa agcgggattc agcgaagagc agcaggccat ctggcaaaaa gcagagctgc
     3781 tgtttggcaa cgagccgtgg tgggtgcgtg acctggcaaa agagacgggt accgacgagc
     3841 aggcaatgcg tctgacgcta cgccaggcgg cgcagcaagg aataattacc gcgatcgtca
     3901 aagatcgtta ttaccgtaac gatcggattg tcgagtttgc caatatgatc cgtgatctcg
     3961 atcaggagtg tggttcaacc tgcgcggcgg atttccgcga tcgcttaggc gtaggccgaa
     4021 agctggcaat tcagattctg gaatattttg accgcattgg ctttacgcgt cgtcgtggaa
     4081 atgatcattt attgc
//
LOCUS       DAPGOF010000269         4087 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.274, whole genome shotgun sequence.
ACCESSION   DAPGOF010000269 DAPGOF010000000
VERSION     DAPGOF010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.274"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            30..602
                     /locus_tag="RHV13_004530"
     CDS             30..602
                     /locus_tag="RHV13_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV1243968.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDSTCEVTSD
                     TADQTVPLGKVSKSAFSGVGSMASPQQFSIKLEKCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPITASDPGDYTGTGAAIVATGVGIRIFNQSDNSQVKLYNDSAYTAIEADGTAEM
                     KFIARYVATNATVTAGTANADSQFTIEYKK"
     gene            650..1381
                     /locus_tag="RHV13_004531"
     CDS             650..1381
                     /locus_tag="RHV13_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HDV1243969.1"
                     /translation="MSRKSCLMRLFKTTMWYKLFIAVIFSLFLGQPVLTYAGVVIGGT
                     RVVYLSNNSDKSISVFSKEEKIPYLIQAWVDPFNKDDKNKAPFTVIPPVSRLEPAQEK
                     VLRIIHTKGVSLPEDRESVFWLNIKNIPPSASNKAENSLEIAVKTRIKLFWRPASIRL
                     IPKDAAPKVKWHREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPAN
                     SAGSQMIWRFINDYGAVSEPIKMSL"
     gene            1406..3928
                     /locus_tag="RHV13_004532"
     CDS             1406..3928
                     /locus_tag="RHV13_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDV1243970.1"
                     /translation="MVTTRIAVGLTAGTFLIFSQSLMAEISVFNPALLEIDHQSGVDI
                     RQFNRANLIPPGVYSVDIFINGKMFERQDVTFVQDNPNSDLHACFVAIKKTLTSFGVK
                     VDALKSLNGVDETVCLDPAPRIEGSSWQLDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETNTSYLNLRNGLNIGPWRLRNYSTLNTTDGR
                     AEYNSISTWLQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARAKFSHDDTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTSNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNVMQSYNSRDDEVG
                     GNMSLTYDGTYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRIAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVVTANGKPAPFGAIAAVQNTSSADSGIVGDQGELYLSG
                     LPDKGQVMLSWGENTATKCTFDYSLSIPEGESGLIEQGVTCH"
     gene            3939..>4087
                     /locus_tag="RHV13_004533"
     CDS             3939..>4087
                     /locus_tag="RHV13_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV1243971.1"
                     /translation="MNKYIIQWCFAVFILYFSGATLAAPKGICTPDNGVFHSTLDFSG
                     YLITA"
BASE COUNT         1108 a          897 c          994 g         1088 t
ORIGIN      
        1 aaaggatata tcatatcaat ggagtttcta tgaagcgtaa tattattggc ggtgcattca
       61 cgctggcatc tttaatgctg gctgggcatg cactcgcaga ggatggcgtt gttcactttg
      121 ttggtgaaat agtcgacagt acttgtgaag tgacctcaga taccgccgat caaactgttc
      181 cacttggtaa ggtcagcaaa agtgcatttt ccggggtagg aagtatggca tcaccacaac
      241 aatttagtat taaactggaa aaatgtccgg caacctatac tcaggcagcc gttcgtttcg
      301 atggtactga agcgcctggc ggagacggtg acctgaaagt aggtacacca attactgcaa
      361 gcgatccggg tgattatact ggcacaggag ctgcaatagt tgctactggc gtaggtattc
      421 gtatttttaa ccagtccgat aattctcagg ttaaactcta taacgattcc gcctataccg
      481 ctatcgaagc tgatggcacc gctgaaatga agtttatcgc acgctatgtt gcaacaaatg
      541 cgaccgtaac ggcgggtacg gcgaacgctg attcacaatt taccatcgaa tataagaaat
      601 aatttatcaa gggccttttt attcagttgg ctttggccag ggagtcgtta tgtccagaaa
      661 aagttgttta atgaggctct ttaaaacaac catgtggtac aaattattta tcgctgttat
      721 atttagcctg tttttaggtc aaccagtatt aacttatgca ggtgttgtca ttggtggtac
      781 acgagttgtt tacttgagta ataactcaga taaatctatt tctgtattta gtaaggaaga
      841 aaaaatccct tatctcatac aggcatgggt ggatcctttt aataaagatg ataaaaacaa
      901 agcgccattt acggttattc ctccggtttc gcggttagaa cctgcgcaag aaaaagtatt
      961 acgcattatt cacaccaaag gtgtgtcatt acctgaggat cgtgagtctg ttttttggct
     1021 gaatattaaa aatattccac cctctgcaag caacaaagca gagaactcgc tggaaatcgc
     1081 tgtcaaaacg cgaatcaaac tgttctggcg accggcaagc attcgtctga ttcccaaaga
     1141 tgccgcaccg aaagtgaagt ggcatcgaga agggcgaaac ttaattgcag agaatcccaa
     1201 cccgattcat atcagcgtga tggatgtcat agtggatggc catgatgtgc ctctcaatat
     1261 gatccgtcct tttgaaacgc tcactttacc cctgccagct aactcggcgg gcagccaaat
     1321 gatttggcga tttattaatg attacggtgc agtaagcgaa ccgataaaaa tgtcgctgta
     1381 atcattgcat aaggataggc gtattatggt tacgaccaga attgcggttg ggctgactgc
     1441 ggggacgttt ctgattttct cgcaaagcct tatggccgag atcagtgtgt tcaatccggc
     1501 gctactggaa attgaccatc aatcaggggt agatattcgc cagtttaacc gggcaaactt
     1561 gattccaccc ggcgtctata gcgttgatat ttttattaac gggaaaatgt ttgaacgtca
     1621 ggatgttacc tttgttcagg ataatcccaa ttctgatttg catgcctgtt ttgttgccat
     1681 taaaaaaaca ctgacttcct ttggggtaaa agttgacgcg ctgaaatcgc tcaatggggt
     1741 ggatgaaacg gtctgtctgg atcctgcgcc acgtattgaa ggttcatcct ggcaactgga
     1801 cagcgataaa cttcagctga atatctctat tccccaaatc tacatggacg cgatggctta
     1861 cgattatata agccccacgc gttgggatga ggggattaat gcgctcacca tcaactacga
     1921 tttttctggt tcgcatactc tccgttcgga ttatggttca caagagacaa atacaagcta
     1981 tctcaatctg cgcaatgggc taaatattgg accgtggcga ttacgcaatt acagcacgtt
     2041 aaacaccacc gatggtcgtg cggaatacaa ctccatcagc acctggttac agcgggatat
     2101 tgctgcgtta agaagccaga ttatgatcgg cgatacgtgg acggcgagtg atatattcga
     2161 cagtacgcaa atccgtggcg cgcgtctcta tactgataac gacatgctac ccgccagcca
     2221 gaatggcttt gcacccgtgg ttcgggggat tgcaaagtct aatgccacgg tcattattcg
     2281 gcagaatggc tacgtgattt atcagtcagc cgtaccacaa ggtgcattcg aaatcaccga
     2341 cctgaatacc gccagtacag gtggcgacct tgacgtgact atcaaagagg aagacggtag
     2401 tgaacaacga ttcactcaac cctatgcctc tttggcaatt ctcaaacgtg aaggtcagac
     2461 cgatgttgat gtcagtgtgg gggaattgcg cgatgaagat ggatttaccc ctgacgtcct
     2521 tcaggcgcag atccttcacg gcttttccca cggaatcaca ttgtatgggg gaatgcaggc
     2581 tgctgagaat tacggctctg ctgcgctggg agtcggtaaa gatcttggcg ctctgggcgc
     2641 aatttcattc gacgtgacac atgctcgcgc gaaatttagc catgatgata cagaaacggg
     2701 tcagtcatat cgctttctct attcaaaacg atttgacgac acagacacca gcttgcgcct
     2761 ggttggctat cgttactcca ccgaaggcta ctataccctc aatgagtggg catcgcggcg
     2821 caacagccct gaagactttt gggaaacagg taaccgacgc agtcgcgtgg aaggaacgct
     2881 aacgcagtcg ttgggcagag actatggcaa tttatacctg acattaagcc ggcaacaata
     2941 ctggcatact gatgatgttg aacgattaat gcaatttggc tatagcagta gctggaagcg
     3001 tctctcttgg aatgtttcct ggagttattc caataccgcc cgacaaggga cgagtaacaa
     3061 ccatgccagt gataacacca gtgagcagat ttacatgctt tccttatctg ttcctttatc
     3121 gggctggtgg ggaaatagct acgccaccta ttctgtttcg caaaacgata attccggtag
     3181 ttcccatcag cttggactca gtggtacggc actggaaaga aataaccttt cctggaacgt
     3241 catgcagtcc tataacagtc gcgatgatga agtcggcggt aatatgtcac tgacttatga
     3301 cggaacttac ggcacagtga atggcagtta taactacagc caaaattccc agcgactcaa
     3361 ttatggtatc aggggaggca ttctggcgca cagtgaagga gtcacgttaa gccaggaatt
     3421 gggtgaaaca atcgctctcg ttaaagcgcc aggggccgcc ggtttggaaa tcgataatat
     3481 gcgtggtgca gcaacggact ggcgtggtta cacggtcaag acacagctaa atccttatga
     3541 tgaaaaccga atagcaatca gtgacaacta tttctcgaag tcgaatatag aactcgataa
     3601 taccgtcgtc acgatggttc ccacccgtgg cgcggtggtt aaggcagagt tcgttactca
     3661 tgtgggttat cgcgtactct tcagagtggt gactgcaaat ggcaaaccgg caccttttgg
     3721 cgctattgcc gctgtacaga atacaagctc cgctgattca ggaattgtcg gtgaccaggg
     3781 ggaactttat ctctcagggc ttcctgacaa ggggcaggtg atgctctcct ggggggaaaa
     3841 taccgccact aaatgcacct tcgattattc actttcaatt ccagaaggtg aaagtggatt
     3901 aattgaacaa ggtgtgacat gtcattaaaa ggatttttat gaacaagtat attatacagt
     3961 ggtgttttgc tgtttttatt ctctacttta gtggtgcaac acttgcagcc ccaaaaggta
     4021 tctgtacccc tgataatgga gtgtttcata gtacgcttga tttctcaggg tatctcatta
     4081 ccgcgaa
//
LOCUS       DAPGOF010000270         4087 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.275, whole genome shotgun sequence.
ACCESSION   DAPGOF010000270 DAPGOF010000000
VERSION     DAPGOF010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.275"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..213)
                     /locus_tag="RHV13_004534"
     CDS             complement(<1..213)
                     /locus_tag="RHV13_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDV1243972.1"
                     /translation="MKIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDI"
     gene            complement(229..2709)
                     /locus_tag="RHV13_004535"
     CDS             complement(229..2709)
                     /locus_tag="RHV13_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDV1243973.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFRGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNLRRISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     STPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANL
                     NWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIASPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAINKQQGLSCTITFGKEIDESRNYICQ"
     gene            complement(2725..3399)
                     /locus_tag="RHV13_004536"
     CDS             complement(2725..3399)
                     /locus_tag="RHV13_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDV1243974.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPVGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSINVKSNNANNWYLTIIDDHGN
                     YISDKI"
     gene            complement(3480..4022)
                     /locus_tag="RHV13_004537"
     CDS             complement(3480..4022)
                     /locus_tag="RHV13_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV1243975.1"
                     /translation="MKHSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
BASE COUNT         1148 a          991 c          756 g         1192 t
ORIGIN      
        1 aatatccgac gggctcatag gggtgatagt atttgtactc acgcatgacg aatcattaaa
       61 attatgctca taggtcgcca tttgtgtttg agtaagtgca aaatccccct gaacaaccaa
      121 attgtgctga acagataact cactataaca tgctgcatag ctcactggca taaccatcat
      181 cattaatata aatagcatta tgcgaatttt catagaacca cctgactatt actggcaaat
      241 ataattccta ctttcatcaa tctctttacc gaaggtgatt gtgcatgaaa gtccttgttg
      301 cttattaatt gccacattaa ccgatggcgg tacttcattg gtgcgaataa ataactgact
      361 tccctggccg acaacgccaa tattatgacc gtggatatca ttgacttcat aaccaaacgt
      421 taatggttgc ccatccgctc ttaacgcttt tataaaccag ggcttgcgct gatcggtatc
      481 aaaattaacc agtacaaccg cgccgcgata aggtgaggca attttccggt taccgcgcaa
      541 ttctgcttcg ctatcgcttt gcgacacatc cagcatcagg tgattttccc gataaggtgt
      601 cattccgtcg tataccacca ctccattacg gtttgttgtg cgatattttt gcccattgac
      661 ataagcatct ttaattcctg gcgcattcat cacagcaaac gtttcggaaa gacggttcgc
      721 cagattaacg ccacccgacc aggcgacaat gccccctgaa acactggctc cagcctgtcg
      781 ataagtactc gactgactat aactgccatt cactgttgcc accggcgcgt tccagtttaa
      841 attcgctcca gctgtcgttt catttccctg atgttgatga ctcaggttga caccataatt
      901 gaactgatcc cgactcccta ctgttcctga taatcccgta ttatttgagg caaacccctg
      961 atcatcaaac gtcgttgagt tagacatata tatttgccga cgaggcgtcg aaacgtcatc
     1021 accccaatca aagggaatcg atataaaaat attaaaacgt ttctcttcat gatgattctc
     1081 gtcataagcc tggcttgccg cgagggtata gcttatccgt cgcaggttgt tggaataact
     1141 caactgataa tccttactac tgccgctacg cccccagtaa tctcgccata acgtacttaa
     1201 tgacacagaa ccccaacctt ctggcaatga ctggctcata ttggcggaaa agctattttt
     1261 gcgaccaaaa tcattctggt aataatcggc aatgtcatag acatcgtttt catcacggcg
     1321 ataattatct ttattgtttg cccaaacgtg atcgttaaat gtccggtagt cacgcgacga
     1381 ataacgccag gccgccagac caaaacgcgt cgacgtttgg ctcacaaatt tgttgtaggc
     1441 aatttgataa ctttgcccgt caaacacatc gccgttgtct tgtttactat gcgacttcgt
     1501 ggcatcgacg gaaatggcac caatgcgtgt attccagcca gtccccaaag tgaacgcgta
     1561 ataattattc gcgaccatcg agccaccata cagcgtcaat aagttattaa aaccatactg
     1621 ataacccgcc tggacaaaat cactttgttt gctcgcccct tcaatatggc tacgacccgc
     1681 cgcaaaatca tatttcgaca cgccgggttg cagcatattt ggcacggctg cataaggcac
     1741 cagataggtg gttaccgaac cgtccgcctc tttcacgctg acatcaagat ctgctccacc
     1801 accggccaac tgcaaatctg taattgcaaa cgggccagga ggaacctctt tctgataaac
     1861 cacaaaacca ttctgttcaa tagttaccag cgcgttactc tgagcaatcc cctgcacccg
     1921 tggcgtaaaa ttttgtttcg agttaggcaa catctgcata tcacgaaaca accgcacacc
     1981 tctgaagcga acagaatcaa aaatatcgct tgatgtgtac atatcaccca cgcgaagcgt
     2041 gccgagaagt tgggcaaatc cacgttccag atacagggta ttgcttttcc acacccctgg
     2101 attgttattt gttttactga aactggcatc agaatgcagt tgccacccca gtaaatttaa
     2161 cccgctgtta aaacgtacat atgtactctt actattaccc gacgctttat agtcactgta
     2221 atactgactc acataataag aggtataaaa cgcattaata ccccgctccc agttttccgg
     2281 tggaacatag ccactttcca gttcttccac ccaggcctgc gggacactga aatcgagacg
     2341 aaaaaccccg atatcccagg tatagctccc accctgaaca agttgctcaa atgttaaaca
     2401 ttgcttaccg ctggcgaagt tatcgctatt aatgcctaac cgcttgataa cttctcttga
     2461 taaacatgtt tcttgcgggt tgtctttaac aataatctca tatttcccgc gccattgctt
     2521 attgacataa atatcgatgt catactgccc cggtaagggt tgattatcat caagacgaat
     2581 atttgcaacc tgctggtctt tcattccacc tatcataaaa tgggtatcaa aggtttcttc
     2641 agctgcataa gcttcaatgc cgagcaataa cgctacgatt gctgatgcaa gtggggtcat
     2701 tctcaacata ggcagctcct gcaattaaat tttgtcacta atatagttgc cgtggtcatc
     2761 gataatggtc agataccagt tatttgcatt attacttttg acattaatac tctgactttc
     2821 taagggggca atcataatag tttcataatt gactttaaca ttattagctt tgacatccga
     2881 aatcgtcacc caattagctg agtcattttt tatcaccaat ccattgccac tgcgatttac
     2941 cagtaacttt ttaaaggtcg ctttatttac cggagcaata cccaccggcc ggtaaaacaa
     3001 tttgattctg ttttgcatcg caaacttcag tgcattctta ccttcttgtt ctggactatt
     3061 tggtggaata tccagaacat tcagataaaa aatgctttct ttattagtgg gcagtttatt
     3121 cggcataatt ttgattttta cttgctgccc ggaatttgcc cctatttttg ccactggtgg
     3181 cgttaacatg aaaggaacct gaattttttc tggtggtaac gacgtatcgc catcatcaat
     3241 ccacgcctgc agcagcgaag aacggtttcc ctggttcatc aactgcacca taacttcttt
     3301 attttctgcc gggtaaataa tgcgcgtccc gtagataaca attccggcat gtgcaggaag
     3361 gaccatagaa aaaatgagta aagataataa tcctttcatc ttaatgcctc ttaaattaaa
     3421 aagccgccat ggggatagcg gccatgttta ttacctggag aaacttgcaa ttttatttat
     3481 taatcctgcg ccacacgcat aaccagagaa gataacacat cacctgattg cactgagtta
     3541 tctgtcagtt tcaggtattt tgctttaaac ggaatagcat attgaccttc cgctgtttta
     3601 taactctcaa tattaaaagg tgcagaaaca tccaggacat cgctgttatc acctaaatag
     3661 agatagtaac ccacagtatc gttgctactg gctatcgcct tatcgttacc agttatttta
     3721 tcgttggtaa tcaacgagag ttttacacca gcaacttctt tcgggcagct aatttttaac
     3781 atgctgctgg ttacaccgac tgcatcatca taggtagaat atttagcttt accttcaaag
     3841 acagtggttg gtacagtcgg catattaata cttactgaat caccacccgc ttcgaattta
     3901 cacggagatt ctgcaatatt ccctttaata gttaatgttc cggtatcaac gtctgcagca
     3961 aatactccag cgctcataaa aaaagaagaa aagacagcgg cagcaatgat tgaatgtttc
     4021 ataatatgtt ccttaataaa ctttattttt ggttgttaag agtctgtatc tcttggaaac
     4081 aaatttc
//
LOCUS       DAPGOF010000271         4039 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.276, whole genome shotgun sequence.
ACCESSION   DAPGOF010000271 DAPGOF010000000
VERSION     DAPGOF010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.276"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..571
                     /locus_tag="RHV13_004538"
     CDS             <1..571
                     /locus_tag="RHV13_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243976.1"
                     /translation="AMVLLFLYINKDNIKVIYSLKINQTTPGILVNCDSNNNFACQTT
                     MTEDVIQRITTFFQTSPDVKNREIKLEWSGDKRDLPTAEAEISRVQASIINWYASEYH
                     NGRQVLDEIQTPSAINSELYTKMIYLTRNWSLYPNGDGCVTISSPEIKNKYPAAICLA
                     LGFFLSIVISVMFCLVKKMVDEYQQNSGQ"
     gene            complement(814..1509)
                     /locus_tag="RHV13_004539"
     CDS             complement(814..1509)
                     /locus_tag="RHV13_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HDV1243977.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGADNISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1502..2929)
                     /locus_tag="RHV13_004540"
     CDS             complement(1502..2929)
                     /locus_tag="RHV13_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HDV1243978.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2940..3659)
                     /locus_tag="RHV13_004541"
     CDS             complement(2940..3659)
                     /locus_tag="RHV13_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HDV1243979.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPMYLADEPEWASRAA
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1068 a         1061 c          846 g         1064 t
ORIGIN      
        1 ggctatggtg ctgttgtttc tgtatatcaa taaagacaat ataaaagtga tttacagcct
       61 aaaaataaac cagacaacac ccggcatact tgttaactgt gatagcaata ataactttgc
      121 ctgtcagacc acaatgactg aagatgttat tcagcgaatt actacatttt ttcagaccag
      181 cccagatgtc aagaacagag aaataaagct ggaatggtca ggagataaga gagatttacc
      241 aactgctgaa gcggaaatat ctcgcgtgca ggcgtctatt atcaattggt atgcgtcaga
      301 ataccataat ggcaggcaag ttctcgatga gatacaaaca ccttcagcaa ttaacagtga
      361 gctttataca aaaatgatat acctgaccag gaactggtca ttgtatccga acggtgatgg
      421 ctgtgtaact ataagctcac cagaaataaa aaataaatac cctgctgcca tttgcctggc
      481 tctgggattt tttctaagca ttgtgatttc tgtaatgttt tgccttgtca aaaaaatggt
      541 agatgaatac caacaaaact ctgggcagta ataaacagca ttctgataga gagtgcctgt
      601 ataaagctga agtgacaatg accgggaagg gaacccgttt tcaggaatgg atgcctgtct
      661 ttttcactgt aaatatcctg gcattactaa tgccaggata tttacagaag agcagcataa
      721 ccatgacgga acggggttaa gtcaggttgt ccttaacgct attcacaacc tcgtatgccg
      781 ccttcaggtt tatgtccaga cttcatatct ctctcagcaa tcctcaataa tcagatacac
      841 cgctttcacc ggaccatgaa ccccgacgac tttgataagc tcaatatccg ccgttgaact
      901 agggccgcta atgatgttaa tgcaggacgg catgcgttca ccggcctgcg ctttctgatg
      961 caatttttct gcgagttgcg ctacacgcgg caggattgtg cttttacgca ggataaaaag
     1021 agaagattcc gggagcaggc tcaatgaacg cccgcgctcg gcggcggaaa aaagaaccac
     1081 gcctcccgat tcggttaaac catattcagc atacacgaca cccactttag cctgctctgc
     1141 ctgcgagata ttatcggcac ctttcgccgg atcccaaaca acggcattgc attcctgctg
     1201 caaacgttcg ctaatcccca attcctccag cctcgtgtca ccgctaatca cgaccgactg
     1261 atctcccagc tctttacaca gacgtattgc agcttctgcc gccttcgcct cgctggtcag
     1321 ttcacagcgc gtcaacataa catcgctggc aaactgaata aacgcgtcac agcgctgctg
     1381 ttggttaagt tgggtaagcc gctcgttagc atagttgtta agcggcgcat cttctgcttg
     1441 cggttcaagt cgtaacgggc gacccagtgc ctgagcaacg ttattcaaaa attcgcctcg
     1501 attatccatt ctttttctcc tgcgcctgat gtttcttaaa ccaactacgg aaactctctc
     1561 cgtcagcttc aggaagatcg cgtgcttcca tccagtcgct aatcgcgcca aatttgagtg
     1621 gtgttttgcc gccattgata aaccagcttg ccgcatgagc gccggccatc atcccgactt
     1681 tccacaatcc tggatgacta ttggcataag cgaacatttt tatcgcccgt tgctctgctt
     1741 ttgcggtgat ccctttttca gccatcaccc gacgatgacg caaaatcagt tttgacagcg
     1801 gaatacgcac cggacacacg ctgtcacaag cagtgcataa agagcaggcg tagggtaaat
     1861 ctttaaaatc tttatagccg ccaagtagcg gagaaatcac cgcaccaatt ggccctggat
     1921 aaatagagcc atatccatga ccgccaatat ggcgatatgc cggacaagta ttcatacaag
     1981 ccccgcagcg aatacagcgc agcacatccc gaaattcaga ggccagcacc tcagaacgcc
     2041 cgttatcgac aataaccaga tgaaactctt caggaccatc aacgtgcccc gcttcgcgcg
     2101 gtcctgtcag ccaggtgttg tatcccgtca aacgtgcacc aacggcactg cgcgccagca
     2161 tggtgatcaa tacatctacc tcggcaaacg tgggggcaat acgctccatt cccatcactg
     2221 caatatgcgt tttaggcagc gtggtacaca ttcgcgcatt accttcattg gtcaccaggc
     2281 ataccgaacc ggtctctgcc accgcgaaat tacagccggt aatacctatt tcagcactga
     2341 ggaaatcttc gcggattttt tgccggatga ataaggtcat cgcttcaggc gtttccggcc
     2401 cctcatagcc cagacgttcg tgtagcactc gacggatttg atggcgatct ttatgaattg
     2461 ccgggaccac aacatgagat ggcggatctt gatccagctg gagaatatat tcacccagat
     2521 cggtttcaat cacctgaatg ccagcatcct gcaacacatg attgacacca atctcttcgg
     2581 tcaccatcga tttagatttc accaccttcc gggcattttt gcgttgggca acctgtaaaa
     2641 tgtagcgggt agcgtcttct ttggtttttg caaaatagac gtgaccgccg ttttgcgtca
     2701 ctttttctga gagctggtac agataagcgt cgagattact cagaacatga tcacgtatct
     2761 gggcggcccg atcgcgccac tcctcccagt gccccaattc atcgaccatt ttttgccgat
     2821 ttgccccaat acgctgctgc gcgtttgcca ccgctttgcg catgatcgga tcttcaattt
     2881 gctgacggat gcgtgtctta aaatctgtat tactggtttt gatcgacatc tttctatcct
     2941 cagcggctca tcaacacttc agcaatatgc atcactttga ctttctgccc ttcccgttgt
     3001 aatcgcccac tgatgttcag caggcaactc acgtcagcac caattaaata ctcagggcgg
     3061 acttccatca ggtgcgcaac cttttctttc accatctcgc cggatatttc ggccattttg
     3121 accgagaacg tgccgccaaa tccgcagcag gtatcctgtt cagcaaaggt caacagctcc
     3181 agtccacgca catttttcag cagcgtaagt ggctcgtcct tcactcccag cttacgggcc
     3241 aggctacaag atgggtgata caccgctctc ccttgcaaac tggcacctac atcgactacc
     3301 cctaatttat taacaataaa agaggtgaga tcctgcatac gcgcggcaac ctttgcggca
     3361 cgtgatgccc attcaggttc atccgccaga tacatcgggt aactttttac ggcataggtg
     3421 caggagccag ccggtgaaat aatgggatca tcgttatcct ccagtgcggc gatcagattt
     3481 ttcatccctg gaatcgcttc tttgatataa ccactattga tcgcaggctg accgcagcat
     3541 ccctgtttct ccgggaaatt tacgcgacag ccgagttttt ccagtagcag cacggagtct
     3601 cgtgccattc ttgatttcag ggcgtcacca atacaggtga caaagaaatt gacattcaca
     3661 actaatactc cattacttca tgcccattta ttcccgatgg ttcccccctt ccgggggatc
     3721 aatcatcaat aaatatggca ttctttttta attggctagc aacataccac ccctcatttg
     3781 tatgacaaat gataatcttt catccaaact tcaaatgcaa taactaattt tatttctttc
     3841 ggcatcgctc acattttatg gggaaatcga cgatcatttt tggcataaga aatccattta
     3901 aaaatggcta catcccttat acctattcag agctacttat tcaggggtta ttgagatata
     3961 cactgatggt gtacaaaaaa atcaccgaca ataatcacgt atcaagtaat agtcgggatt
     4021 ttatttaatt gaagcaatt
//
LOCUS       DAPGOF010000272         3995 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.277, whole genome shotgun sequence.
ACCESSION   DAPGOF010000272 DAPGOF010000000
VERSION     DAPGOF010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.277"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..2000)
                     /gene="rsxC"
                     /locus_tag="RHV13_004542"
     CDS             complement(<1..2000)
                     /gene="rsxC"
                     /locus_tag="RHV13_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HDV1243980.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVTDPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEQQVDPRKAAVE
                     AAIARAKARKLEQQHANAEPEEQVDPRKAAVEAAIARAKARKL"
     gene            complement(1993..2571)
                     /gene="rsxB"
                     /locus_tag="RHV13_004543"
     CDS             complement(1993..2571)
                     /gene="rsxB"
                     /locus_tag="RHV13_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HDV1243981.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(2571..3152)
                     /gene="rsxA"
                     /locus_tag="RHV13_004544"
     CDS             complement(2571..3152)
                     /gene="rsxA"
                     /locus_tag="RHV13_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HDV1243982.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(3229..3669)
                     /locus_tag="RHV13_004545"
     CDS             complement(3229..3669)
                     /locus_tag="RHV13_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HDV1243983.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGAQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALFVPYFKRSSRVKATFVNP"
     gene            complement(3755..3970)
                     /gene="ydgT"
                     /locus_tag="RHV13_004546"
     CDS             complement(3755..3970)
                     /gene="ydgT"
                     /locus_tag="RHV13_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HDV1243984.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
BASE COUNT          994 a         1066 c         1030 g          905 t
ORIGIN      
        1 agcttgcgtg ctttggcacg ggcaatagcg gcttcgacgg cggctttgcg cggatcgacc
       61 tgttcttctg gttccgcatt agcatgttgc tgttccagct tgcgcgcttt ggcgcgggcg
      121 atagcggctt cgacggcggc tttgcgcgga tcgacctgtt gttcaggttc cgcattagcc
      181 tgttgctgtt ccagcttgcg tgctttggca cgggcaatag cggcttcgac ggcagctttg
      241 cgcggatcaa cctgttcttc tggctccgca tttgcctgtt gctgttccag cttgcgtgct
      301 ttggcgcggg cgatagctgc ttcaacggca gttttacgtg gatcagtaac ggttgctgcg
      361 tcgttagttt gctgcagttc tgcctgtttc gctctggctt gcgctttacg ggcttcccgt
      421 gctgcaataa ttgcactgtt atccgggcgt tcgcccgctt taatcacaat aggctgtgta
      481 gcctgggcct gtttctcttt cacccgcgcc agagcggcag caatcgcatc tttatctttg
      541 gctgcaggtt gaacggctgc gctcttatgt cgttcaaggc gagccgcttt ttcgcgctcc
      601 agacgagcct ggcgcgcttc gaaacgcgct ttggcttctg cggcgcgctt ttcttcctga
      661 cgaatagccg caatttcagc tttttcctga cggaaatatt gcaccagggg aatattgctc
      721 gggcaaaccc acgcgcaagc cccacattca atgcagtcgg caatgttatg cgtggtagct
      781 ttatcgtgtt gctgaccttt gctgaaccag tacaactgtt gcggcaaaag atccgcaggg
      841 caggcgtcag cacaggcgct acaccggatg cagctttgtt cttcctgtgg ttcgccaagc
      901 tcattggcag agggagccaa cagacagttg gtaatcttta cgaccgggac atccagccat
      961 ggcaaggtaa agcccattag cgggccaccc ataatcacca tttgatcggc agaggggcag
     1021 aatccggcat cattcaataa atgacgcact ggcgtcccca gccgtgccca gacgttgccc
     1081 gggcgagcga ttgcttcgcc agtcagggtt acaacacgct cggtaatcgg ctcgccatca
     1141 ataacggcac gtttcactgc ataagcagtg ccgacgtttt gcattaatac gccgatatcg
     1201 gatgaacgcc cgccatgtgg aacctgcttc ccggtcagaa tgtaggttaa ttgtttagca
     1261 ccgccagaag gatatttggt tggaatcacc cgcagagaaa tatcgttaga gtccgccagc
     1321 accgcgcgca gcatggaaat cgcctgcggt ttgttatctt caatgccgat aagaatttcg
     1381 cgtggctgca gaatatgcgc aagaatgcga ataccctcta cgacctgagc cgcgcaatcc
     1441 tgcatcaaac ggtcatcggc ggtaatgtac ggctcgcact cagccgcgtt gataatcaac
     1501 gtttcaatct tatctccgcc accctgcaat ttaacgcctg tcgggaatcc tgcaccgccc
     1561 agcccggcaa caccaaactg atgtatgcgc tcgattaact cttcgcgact gcgagtgcga
     1621 taatcggccc agccgtcgcg cgggatccag cagtcttcac catcggcatc aataatcacg
     1681 cttaattcag ctaaagctga aggatgagcc gtagagtggg gcgcaatagc cgtaacggta
     1741 cccgaggtgg gcgcgtgaac aggcagcatt ttgccgcgac cacgggtaag cggctggccg
     1801 cgcaatactt tatcgccgac gctaacgcac aactcacctt cagcgccaat atgctgtttc
     1861 agtggaataa caaaacgctg cgccagtggt acctggcgca ggggtgtacc gttggactgg
     1921 gttttcatct ccggtggatg gatgccgccg ttgaaatccc agattttatt ttttctgaat
     1981 gcagagaata acttaagcat ggtgttccac gggaatgata cgcacgggaa tggtgttcag
     2041 atcccatttc caggagtcag gtgtttctgc gaccggttgc aacgagatgc agtgcgtcgg
     2101 gcacggatca acacataaat tgcagcccgt acagagatca ctcattaccg tatgcatggc
     2161 acgggtagcg ccaacgatgg cgtctaccgg acaagcctga atacatttag tgcagccaat
     2221 acagttattt tcatcaataa ccgccaccat ccgcgcaggc gttagctctt gcgcttcgcc
     2281 atccagcggc tgcggttcga cattaagcaa ctcggcaatt tttagcatca cagcttcgcc
     2341 accaggggcg caacggttga ttttttcacc gttacagctg atggcttccg cgtaggggcg
     2401 acagccggga taaccgcact gaccacactg gctctgcggt aagatttcgt caattttctc
     2461 aacgaccgga tcgtcttcca ccgcaaaacg gcgggaggca taacccagaa tggcgccaaa
     2521 cgccaggccc agcaggctca cggcggcaac ggcaatccag atagcattca ttacaacttc
     2581 accaaaccac taaagcccat aaaggccaga gacataagac ctgcggtaat taacgcaatg
     2641 gcattaccgc gaaaaggtgc cgggacatca gccacagcaa ggcgttcgcg gatagctgcg
     2701 aagagaacca tcaccagcga aaaaccgaca gcggcggaaa aaccgtacag cgccgactgc
     2761 aagaaattgt gcccgagatt gatattcagc aacgccacgc cgagcacggc acagttggtg
     2821 gtgataagcg gcaaaaaaat ccccagcaaa cggtaaagca ccgggctggt tttgcgcacc
     2881 accatctcgg tgaactgcac gaccacagca atcaccagaa taaatgccag ggtgcgcagg
     2941 taaatcagat taagtgggat caaaatccac gtatcgataa gccaggcgca aatagacgcc
     3001 agcgtcatca caaacgttgt tgccagcccc atgcccatcg cggtttccag ctttttggaa
     3061 actcccataa acggacagag accgagaaac ttgaccagta caaagttatt gaccagtaca
     3121 gttccaacaa acagtaacag gtagtcagtc atttttcggc ctgaaataaa aaagccgcct
     3181 attatccgtt aatccagagc aggcgacaac aggttaactg taaggttatt acggattcac
     3241 aaacgtcgct ttcacccgcg acgagcgttt gaaatagggg acgaacaatg cggttgccaa
     3301 caaagtaaac agcaactggc gaacggctat gccatcttcc acgggtgaaa aagcgaacgc
     3361 ttttactgcc agcaatacgg aaataagcag ccagataata tagtgtttag gaacacaacg
     3421 ccgacgttta aagaaagcga tggtcaacca cagtgtgtaa taccacatgg cgatagcggt
     3481 aataaaagaa acgccccata agatttgtgt cgtcagtgct tgtgcgccga gcgtttgaaa
     3541 tgtttgcgga gaagataacg cagcggtata cagcaacaac gccagcgtcg tacttaacag
     3601 agcgactaaa agccaggcca acgggccaag caaccagcct ccaatacgtt gtggcgttgt
     3661 ggtggtcata ggttctccag aatagcgacg caaacaggca aatctaattt taggggcgtt
     3721 attataaggc ttttataacc gatcgctact ttctttattg gacatagtgc cagacggact
     3781 tcggtacctg gccaaggtca aacaaacgcc cgccagaaac cagctctgcg cgacggtgat
     3841 cggcagcacg atacatattg atcagttcct gatcgtccgt cagggtgtaa ttaagatggt
     3901 cgtagagttt ttccagactt tcgagtgaac tgattttgcg aaattttaat aagtagtcct
     3961 gaacagtcat aaaaggtcca taaaataatt aatac
//
LOCUS       DAPGOF010000273         3878 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.278, whole genome shotgun sequence.
ACCESSION   DAPGOF010000273 DAPGOF010000000
VERSION     DAPGOF010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.278"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(140..1489)
                     /gene="lysC"
                     /locus_tag="RHV13_004547"
     CDS             complement(140..1489)
                     /gene="lysC"
                     /locus_tag="RHV13_004547"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HDV1243985.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            2014..3663
                     /gene="pgi"
                     /locus_tag="RHV13_004548"
     CDS             2014..3663
                     /gene="pgi"
                     /locus_tag="RHV13_004548"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDV1243986.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
BASE COUNT          988 a         1024 c          987 g          879 t
ORIGIN      
        1 agagatgcag ataaaaaaat taaaggcaat tattctccga taaagacgtt ggttaacaga
       61 gtacaggctc gtttattgat gagcatagtg acaagaaaat caatacggcc cgaaatatag
      121 cttccaggcc atacagtatt tactcaaaca aattaaaatg cagcttctgc accacctgct
      181 cggcatcttc gccgggcacc aggaagcaca ggttatggct ggatgcgcca taacaaatca
      241 tgcgaatgtt gaacggttcc agtacgccga atacctcttt gccaacgccg caggcttttg
      301 acaggtcatt gccaatcaac gcgaccagcg ccagaccttc ttccacctcc acccggcaca
      361 gtgcggaaag ctccatcagc agagattgcg tcagcaacgt atcgccagtg gaggttgaac
      421 cggtggtatc aagggttaat gccacgctca cttctgacgt ggtgattaag tctaccgaaa
      481 tgttatgccg cgcgaggatg ccgaaaactt ccgcgaggaa gccgcgagaa tgcagcatat
      541 tcaggctgtg caaagtgagc agagtctgat tgcgacgaag cgccagcgcg cggaacagcg
      601 gcggattttc agttttattg cacaccagcg taccacctgc gcgtgggtct ttgctggagc
      661 cgacaaagac cgggatatcg ctgcgtactg cgggtagcaa tgttgccgga tgcagtactt
      721 ttgcaccaaa agttgccatc tctgctgctt cggcaaacgc gatttcatca atgcgttttg
      781 ctgcggaaac cacgcgtgga tcggtggtgt agatgcccgg gacgtcggtc cagatatcaa
      841 cacgagatgc gtgtaaagcc tccgccagca aggctgccgt ataatcgctg cctccacggc
      901 caagcgtcgt tgtacgacct ttattttcgc taccgataaa tccctgggtg atcactaagc
      961 cttcattgag gcgtgggagc agctgcagcg cggccagttc cgccagcgcg gctacatctg
     1021 gctctgcacg accaaatcgg tcgttggtac gcatcacttt acgtacatca aaccactgtg
     1081 cctgaacatc gcgttcgcgc aggatctcga caaacagcag ggtcgacatc agctcgccgt
     1141 ggctgaccag ttcatctgtc agcgccggag acgttgccag cgccgccgct tctgccagaa
     1201 cagtaatgtt ctccagcaga cgttcaatct cttcacggat aacgttcggg taacgcagac
     1261 gttccagaat ggcaaactgg atgttgcgga tagcgtcgag tttttcgaat cgctcgccag
     1321 gttccagtcc ttcagctaaa gcgactagca gattagtgat accagcagaa gccgagagga
     1381 caactaaacg cacgttggca tcagaaagca caatatcagc gctgcggttc atggcgtcaa
     1441 aatcagctac gctggtaccg ccaaatttgg agacaacaat ttcagacata actacctcgt
     1501 gtcaggggat ccattttcag ccttggcaca agggaagagc ggaagacggg tgggcgcaga
     1561 gcgatacttc gctactattt tcacccagaa gtgctccacc acttgcgaaa cgcccgactg
     1621 cgaacgcttc tggtgacaac ccaggggatt cagcccctgt agccgatgat gaacgtggcc
     1681 agccgttcaa tcacctcggc gatgcacccc ctcaggtgtt atcacaggac tggctcctcc
     1741 aacaccgtta cttgggcaac gcgcctcttc tggcctgcgc tagcgcaggt agtacattta
     1801 taaataaagg gtgagcgggg cggttgtcaa cgacggggtc atgcggattt ttcatccact
     1861 cctggcggtc agtagttcag ctaataaatg cttcactgcg ctaagggttt acactcaaca
     1921 ttacgctaac ggcactaaaa ccatcacatt tttctgtgac tggcgctaca atcttccaaa
     1981 gtcacaattc tcaaaatcag aagagtattg ctaatgaaaa acatcaatcc aacgcagacc
     2041 gctgcctggc aggcactaca gaaacacttc gatgaaatga aagacgttac gatcgccgat
     2101 ctttttgcta aagacggcga tcgtttttct aagttctccg caaccttcga cgatcagatg
     2161 ctggtggatt actccaaaaa ccgcatcact gaagagacgc tggcgaaatt acaggatctg
     2221 gcgaaagagt gcgatctggc gggcgcgatt aagtcgatgt tctctggcga gaagatcaac
     2281 cgcactgaaa accgcgccgt gctgcacgta gcgctgcgta atcgtagcaa taccccgatt
     2341 ctggttgatg gcaaagatgt tatgccagaa gtcaacgcgg tgctggagaa gatgaaaacc
     2401 ttctcggaag cgattatctc cggtgagtgg aaggggtata ccggcaaagc aatcactgac
     2461 gtagtgaaca tcgggatcgg cggttctgac ctcggcccgt acatggtgac cgaagcactg
     2521 cgtccgtaca aaaaccacct gaacatgcac tttgtttcta acgtcgatgg gactcacatc
     2581 gcggaagtgc tgaaaaaagt aaacccggaa accacgctgt tcctggtagc atctaaaacc
     2641 ttcaccactc aggaaaccat gaccaacgcc catagcgcgc gtgactggtt cctgaaagcg
     2701 gcaggtgatg aaaaacacgt tgcgaaacac tttgcggcgc tctcaaccaa tgccaaagcc
     2761 gttggcgagt ttggtattga taccgccaac atgttcgagt tctgggactg ggttggcggc
     2821 cgttactctt tgtggtcagc gattggcctg tcgattgttc tctccatcgg ctttgataac
     2881 ttcgttgaac tgctttctgg cgcacacgcg atggacaagc atttctccac cacgcctgcc
     2941 gagaaaaact tgcctgtact gctggcgctg attggcatct ggtacaacaa tttctttggt
     3001 gcggaaactg aagcgattct gccgtatgac cagtatatgc accgtttcgc ggcgtacttc
     3061 cagcagggca atatggagtc caacggtaag tatgttgacc gtaacggtaa cgttgtggat
     3121 taccagactg gcccgattat ctggggtgaa ccaggcacta acggtcagca cgcgttctac
     3181 cagttgatcc accagggaac caaaatggta ccgtgcgatt tcatcgcccc agctatcacc
     3241 cataacccgc tctctgatca tcaccagaaa ctgctgtcta acttcttcgc ccagaccgaa
     3301 gcgctggcgt ttggtaaatc ccgcgaagtg gttgagcagg aatatcgcga tcagggtaaa
     3361 gatccggcaa cgcttgacta cgtggtgccg ttcaaagtgt tcgaaggtaa ccgcccgacc
     3421 aactccatcc tgctgcgcga aatcactccg ttcagcctgg gtgcgttgat tgcgctgtat
     3481 gagcacaaaa tctttactca gggcgtgatc ctgaacatct tcaccttcga ccagtggggc
     3541 gtggaactgg gtaaacagct ggcgaaccgt attctgccag agctgaaaga tgataaagaa
     3601 atcagcagcc acgatagctc gaccaatggt ctgattaacc gctataaagc gtggcgcggt
     3661 taatcatcgt cgatatgtag gccggataag gcgttcacgc cgcatccggc aaccgatgcc
     3721 tgatgcgacg cggtcgcgtc ttatcaggcc tacaggtcga tgccgatatg tacatcgtat
     3781 tcggcaatta atacatagca cgattgatta aataacctta ataacaatgc cgacgttatg
     3841 tcggcatttt tttatcagat aaatcccctt gtctgtga
//
LOCUS       DAPGOF010000274         3842 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.279, whole genome shotgun sequence.
ACCESSION   DAPGOF010000274 DAPGOF010000000
VERSION     DAPGOF010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.279"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            40..1698
                     /gene="dpiB"
                     /locus_tag="RHV13_004549"
     CDS             40..1698
                     /gene="dpiB"
                     /locus_tag="RHV13_004549"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HDV1243987.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSIISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            1667..2347
                     /gene="dpiA"
                     /locus_tag="RHV13_004550"
                     /gene_synonym="citB"
     CDS             1667..2347
                     /gene="dpiA"
                     /locus_tag="RHV13_004550"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HDV1243988.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2387..3772)
                     /gene="dcuC"
                     /locus_tag="RHV13_004551"
     CDS             complement(2387..3772)
                     /gene="dcuC"
                     /locus_tag="RHV13_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HDV1243989.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
BASE COUNT         1039 a          931 c          996 g          876 t
ORIGIN      
        1 atccgtcaaa gaggctcggg acaacccgca aggaaaacaa tgttgcagct taacgagaat
       61 aaacagtttg catttttcca aagactggca tttccgctgc gtatcttttt gctgattctg
      121 gtgttctcaa tatttgtcat tgcagccctg gcgcaatatt ttacggccag ttttgaggac
      181 tatttaacgc ttcatgtacg cgacatggca atgaatcagg cgaaaattat tgcctccaat
      241 gacagtatca tcagcgcagt aaaaacgcgt gactacaaac ggctggcgac catcgctaac
      301 aaattacaaa gagataccga ttttgattat gtggtgattg gcgaccggca ctcgatccgc
      361 ctttaccatc ctaatccgga gaaaattggt tatcctatgc agttcaccaa acagggcgcg
      421 ctggagaaag gggagagcta cttcattacc gggaaagggt caatgggtat ggcgatgcgc
      481 gccaaaacgc caatctttga tgacgatgga aaagtcatcg gcgtggtgtc aattggctac
      541 ctggtgagta aaatcgatag ctggcgggct gagtttttat taccgatggc aggtgtgttt
      601 gtcgtgctgt tagggattct gatgttgctg tcgtggttcc tggccgcgca tatccgtcgg
      661 cagatgatgg gcatggagcc aaagcaaatc gcacgcgtgg tccgtcagca agaggcgctg
      721 tttagttcgg tttatgaagg gctgattgcg gtggatccgc acggttacat taccgccatc
      781 aatcgtaacg caagaaagat gctggggctg agttcccccg gacggcaatg gttgggtaaa
      841 cccattgctg aagtggtcag gcccgccgat ttctttaccg aacagattga tgaaaaacgt
      901 caggatgtgg tggctaactt taacggtctg agcgttattg ccaatcggga agctattcgt
      961 tcaggtgatg atttgctggg ggccattatc agctttcgta gtaaagacga aatttccacc
     1021 ctcaatgcgc aactgacgca aataaaacaa tacgtcgaga gccttcgtac attgcgacac
     1081 gagcatctca attggatgtc gacgctcaat ggtctgttgc agatgaaaga gtatgatcgc
     1141 gtgctggcga tggtgcaggg ggagtctcag gcccagcaac agcttattga cagcctgcgc
     1201 gaggcgtttg ccgatcgcca ggtggcgggg ctgctttttg gtaaagttca gcgcgcccgc
     1261 gaactggggc taaaaatgat catcgtcccc ggtagccagc tttcgcaact gccgccagga
     1321 ctggatagca ccgagtttgc cgccattgtg ggaaatttac ttgataacgc cttcgaagcc
     1381 agcctgcgta gcgatgaagg aaacaagatc gttgaattat tcctcagcga tgaaggcgat
     1441 gatgtggtga ttgaagtcgc cgatcagggc tgcggcgttc cagagtctct acgagacaaa
     1501 atatttgagc agggggtcag tacgcgtgct gacgagcccg gtgaacatgg cattgggttg
     1561 tacttgattg ccagctatgt aacgcgctgc ggtggtgtta tcactctcga agataatgat
     1621 ccctgcggta ccttattttc aatctatatt ccgaaagtga aacctaatga cagctccatt
     1681 aaccctattg atcgttgagg acgaaacgcc gctggcagag atgcatgcgg aatatattcg
     1741 tcacattccc ggattcagtc agatattact ggcgggaaac ctggcgcagg cccgaatgat
     1801 gatcgagcgt tttaagccgg ggctaatctt gctcgataac tatcttcctg acggtagagg
     1861 gattaattta ttgcatgaac tggtgcaggc gcattatccc ggcgacgtgg tgtttaccac
     1921 tgcagccagc gatatggaaa cggtgtctga agccgtacgt tgtggtgtat ttgattatct
     1981 cattaagccc attgcctatg aacggctggg gcaaacgcta acccgtttcc gccagcgtaa
     2041 acatatgctg gaaagtattg atagcgccag ccagaagcaa attgatgaga tgtttaatgc
     2101 ctatgctcgc ggtgaaccta aggacgagct accgaccggc attgacccct tgacgctaaa
     2161 cgcggtgcga aaactgttta aagagccggg tgtgcaacat acggcagaaa cggtggcgca
     2221 ggcactgacc atcagccgca ccactgccag gcgttatctt gaatattgcg ccagccgcca
     2281 tctgattatt gctgaaattg ttcacggcaa agttggcaga ccgcaacgca tataccacag
     2341 tgggtgacat gataaagccg gaggaaactg cctccggcat tactatttac ttgcctgtga
     2401 ccgctgctgc cgttcctggc accatcagct ctgtagcaac gataacaatc accagcccaa
     2461 caagcaccgg caccgaggtg cgttttacga cttcaaatgg tgagattttc gccatcccgg
     2521 caaccgcaac gactacgcca gaaacgggtg acagggtacg accgaggttt gacgcctgca
     2581 gcatcgggat agtcaaatac gccgggttaa tgccggaaga gtgcgccagt ttcgggatca
     2641 tctcaacaaa cgcataaaac ggcgcattgc ctgaaccggt cgtgactgcc gccagcattg
     2701 tcagaatcac caataccagc atcaggatga tactcgccga accaaacgat gtagcgatag
     2761 aaatcagact ttgaataaag ccgatggtgc taagcccctg agcgaatacc ccagcggcaa
     2821 ccagcagcat caccacgttt gcaaacgcat ctgccatccc gcgataagcc acttccagac
     2881 cagagaaaac tttctgggta ttaaagctgc ggaggaactc cagaatggag gcaatcagca
     2941 tacaaatcac cagaatagtg atgatgtgta attgcggacc ccatttaccg tcaaaaatca
     3001 gtacaccgat gatcggcgtg aacggcaaaa tggcataaaa cgcaggagca gtggtggtga
     3061 tttcactgac atctaacatt tcatgagaga tgtgctcttt tttatccaga taacgttgcc
     3121 agaagaagtg ggcgatcgcc atgccgataa ttgcagcaat tgagataggc agcgtcgttt
     3181 tgaaggcgaa gtcaatcagt gacatttcgg aagcttgcgc cgccagcacc acatcccctg
     3241 aagtcggtgc gagaataatc gccgccgggg aggcacaaat ggcagctgct gcgccacgac
     3301 tgataccaac gtttaccatc accggaaaca gagtagccat cagcaaaacg cccagaccgg
     3361 ttgcggaaga gacggccagt gacatcagac aggcaacaaa ataggcggca atcatcagca
     3421 ggtaggggga gttaatatac tgcaatggtt ttgacgccag cttgaccacc atatcattcg
     3481 cgccgatatg ggtcatgtaa gcggcaaagc cacacagcat cataatcatc atgccgaggt
     3541 cgccgccgcg gctcattagc aatattttaa cgtattcaac aatatccgtg gcgctgtagc
     3601 ctgttgaagc ctggctggac ggtaacactt tgtgccccat aatggcactg ataatcagca
     3661 ataacaggcc accgacaaat aacacaccag tggcggaata ccctttaatg atgtagcgag
     3721 ctacacccac aataaccaca accccaataa ggagttcaat gaatgtcagc ataatttttc
     3781 ctgtctccag ccccaaagta aataataaaa aattcctaaa acttaaggaa aaaatatgcc
     3841 ca
//
LOCUS       DAPGOF010000275         3825 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.280, whole genome shotgun sequence.
ACCESSION   DAPGOF010000275 DAPGOF010000000
VERSION     DAPGOF010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.280"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..463
                     /locus_tag="RHV13_004552"
     CDS             <1..463
                     /locus_tag="RHV13_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV1243990.1"
                     /translation="VVHIQNEEEIDVMLHQNAFVIIDTLMNNVFHSNFLTQIERLKPV
                     HVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALINGSYCSVPEANVSLSRKQHQVLS
                     CIANQMTTEDILEKLKISLKTFYCHKHNIMMILNLKRINELVRHQHIDYLV"
     gene            complement(524..1552)
                     /gene="chuS"
                     /locus_tag="RHV13_004553"
     CDS             complement(524..1552)
                     /gene="chuS"
                     /locus_tag="RHV13_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hematinate-forming heme oxygenase ChuS"
                     /protein_id="HDV1243991.1"
                     /translation="MNHYTRWLELKEQNPGKYARDIAGLMNIREAELAFARVTHDAWR
                     MRGDIREILAALESVGETKCICRNEYAVHEQVGAFTNQHLNGHAGLILNPRALDLRLF
                     LNQWASVFHIKENTARGERQSIQFFDHQGDALLKVYATDNTDMAAWSELLVRFITDEN
                     TPLELKAVDAPVVQTRADATVVEQEWRAMTDVHQFFTLLKRHNLTRQQAFNLVADDLA
                     CKVSNSALAQILESAQQDGNEIMVFVGNRGCVQIFTGVVEKVVPMKGWLNIFNPTFTL
                     HLLEESIAEAWVTRKPTSDGYVTSLELFAHDGTQIAQLYGQRTEGEQEQAQWRKQIAS
                     LIPEGVAA"
     gene            complement(1601..3583)
                     /gene="chuA"
                     /locus_tag="RHV13_004554"
                     /gene_synonym="shuA"
     CDS             complement(1601..3583)
                     /gene="chuA"
                     /locus_tag="RHV13_004554"
                     /gene_synonym="shuA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent heme/hemoglobin receptor
                     ChuA/ShuA"
                     /protein_id="HDV1243992.1"
                     /translation="MSRPQFTSLRLSLLALAVSATLPTFAFATETMTVTATGNARSSF
                     EAPMMVSVIDTSAPENQTATSATDLLRHVPGITLDGTGRTNGQDVNMRGYDHRGVLVL
                     VDGVRQGTDTGHLNGTFLDPALIKRVEIVRGPSALLYGSGALGGVISYDTVDAKDLLQ
                     EGQSSGFRVFGTGGTGDHSLGLGASAFGRTENLDGIVAWSSRDRGDLRQSNGETAPND
                     ESINNMLAKGTWQIDSAQSLSGLVRYYNNDAREPKNPQTVEASESSNPMVDRSTIQRD
                     AQLSYKLAPQGNDWLNADAKIYWSEVRINAQNTGSSGEYREQITKGARLENRSTLFAD
                     SFASHLLTYGGEYYRQEQHPGGATTGFPQAKIDFSSGWLQDEITLRDLPITLLGGTRY
                     DSYRGSSDGYKDVDADKWSSRAGMTINPTNWLMLFGSYAQAFRAPTMGEMYNDSKHFS
                     IGRFYTNYWVPNPNLRPETNETQEYGFGLRFDDLMLSNDALEFKASYFDTKAKDYIST
                     TVDFAAATTMSYNVPNAKIWGWDVMTKYTTDLFSLDVAYNRTRGKDTDTGEYISSINP
                     DTVTSTLNIPIAHSGFSVGWVGTFADRSTHISSSYSKQPGYGVNDFYVSYQGQQALKG
                     MTTTLVLGNAFDKEYWSPQGIPQDGRNGKIFVSYQW"
BASE COUNT          982 a          974 c          878 g          991 t
ORIGIN      
        1 tgtcgttcat atacagaacg aagaagagat cgacgtaatg ttgcatcaga atgccttcgt
       61 cattattgat acattaatga ataatgtatt tcattctaat tttctcactc aaattgaacg
      121 attaaaacct gtccatgtca ttattttctc cccctttaat attaaacgct gcctggggaa
      181 agtgccggtg acctttgttc cgcggactat cactatcatt gattttgtcg cactcatcaa
      241 tggcagttac tgctctgtgc ctgaagcgaa tgtgtcactt tcgcgcaagc aacatcaggt
      301 actgagctgc attgcgaatc aaatgacaac ggaagatatt ctggagaaac tgaaaatatc
      361 gctaaaaacg ttctactgcc ataaacacaa tatcatgatg atcctcaatc ttaagcggat
      421 caatgagctg gtacgccatc agcatattga ttatctggtg tgaacgattg atcaatataa
      481 agaggcccag caataaatag ccagacctcc cgttaattat acgttatgca gcaacgcctt
      541 ccggtatcag cgaagcaatt tgcttacgcc attgcgcttg ctcctgttcg ccttctgtac
      601 gttgaccata aagttgcgct atctgcgtac catcatgggc aaacaattcc agactggtta
      661 cgtagccatc gctggtcggt ttacgggtaa cccaggcttc agcaatgctc tcttctaata
      721 gatgaagagt aaacgtcggg ttgaaaatat tcagccaacc tttcattggc accacttttt
      781 ctaccacacc agtgaaaatc tgtacgcagc cacggttgcc aacaaacacc atgatttcat
      841 taccatcctg ctgtgcagat tcaagaattt gcgccaacgc actgttggat actttgcagg
      901 ccaaatcgtc tgccaccaga ttgaacgcct gttggcgcgt cagattgtgg cgctttagca
      961 acgtaaaaaa ctgatgaacg tcggtcatcg cccgccactc ttgctcgacc acagtggcat
     1021 cggctcgcgt ttgaacaact ggcgcatcaa cggcttttaa ctcaagcggc gtattctcat
     1081 cggtgataaa ccgtaccaga agctcactcc atgccgccat atcggtattg tcggtggcat
     1141 aaacttttag taatgcatcg ccctgatgat caaagaactg aatactctgg cgttcgccac
     1201 gagccgtgtt ttctttgatg tggaaaacac tggcccattg attgagaaac agacgtaaat
     1261 ccagcgcgcg tggattgagg atcaatccgg catgtccgtt caaatgctgg tttgtgaacg
     1321 caccaacttg ctcatggact gcatattcat tacggcaaat acatttggtc tcgccaacac
     1381 tttcgagcgc cgccagaatt tcacggatat cgccgcgcat ccgccatgca tcgtgcgtga
     1441 cgcgtgcaaa tgccagttct gcttctctaa tattcattaa ccctgcgatg tcacgcgcgt
     1501 actttccggg attttgttct tttaactcaa gccagcgtgt gtagtggttc attgtctctc
     1561 ttccttccag ataaatgccc cgaaatatcg gggcagatga ttaccattga taactcacga
     1621 aaatttttcc gttacgacca tcctgtggga tgccttgtgg cgaccagtac tctttgtcga
     1681 aagcgttacc caacactaaa gtagtggtca tacctttgag cgcctgttgt ccttgataac
     1741 tgacgtagaa atcattcacg ccatagcctg gttgtttgct gtaactgctg ctgatatgtg
     1801 ttgagcgatc ggcaaacgta ccaacccacc caacagagaa gccactgtga gcgatcggaa
     1861 tattcagggt gctggtaacg gtatccgggt taatgctgga gatgtattcg ccggtatcgg
     1921 tgtctttgcc gcgggtacgg ttataggcca catcaaggct aaacagatca gtggtatatt
     1981 tcgtcatcac atcccagccc cagattttgg cgttcgggac gttatacgac atagtcgtcg
     2041 ccgccgcgaa atcgacggtc gtggagatgt aatccttcgc tttggtatca aagtagctgg
     2101 ctttaaattc cagagcatca ttggacaaca tcaggtcatc aaaacgcagc ccaaaaccgt
     2161 actcctgagt ttcgttagtt tccggacgta agttcgggtt tggcacccaa tagttggtat
     2221 agaagcgacc aatcgagaag tgcttagaat cgttatacat ttcgcccatc gtcggggcgc
     2281 ggaatgcctg ggcatatgag ccaaataaca tcagccagtt agtcggattg atagtcatcc
     2341 ccgcacgaga tgaccatttg tcggcatcaa catctttgta accgtcactg ctaccgcgat
     2401 aactgtcata gcgggttccg ccaagcaggg taatcggcag atcgcgtaag gtgatctcat
     2461 cctgtagcca gccggagcta aaatcgattt ttgcttgcgg gaagcccgtc gtcgcgccgc
     2521 ccggatgttg ttcctgacga taatactcac cgccatatgt cagtaagtga gaagcgaaac
     2581 tgtcggcaaa gagagtggaa cggttctcca gcctggctcc ttttgttatc tgttcacgat
     2641 actcgccgga actccccgtg ttttgcgcat taatacggac ttccgaccaa taaatttttg
     2701 catctgcatt taaccagtcg ttgccctgcg gggcgagttt ataagaaagc tgcgcatcgc
     2761 gttgaattgt tgaacgatca accatcgggt tgctgctttc agaagcctca acggtctgcg
     2821 gattttttgg ttcacgcgcg tcgttgttgt agtaacgcac taaaccgctc agagactgtg
     2881 ctgaatcaat ttgccaggtc cccttcgcca gcatgttatt aatggactcg tcattcggcg
     2941 cggtttcacc attgctctgg cgtaaatcac cccgatcgcg actggaccag gccacaatac
     3001 catccagatt ttcagttcgc ccaaacgcgc tcgcgcctaa tcccaggcta tggtcccccg
     3061 tgccgccagt accaaagaca cgaaaaccac tgctttgtcc ttcctgcaat aaatcttttg
     3121 catcgaccgt atcgtaggag atcactccac ccagcgcgcc actgccatac agtaatgctg
     3181 aaggtccacg aacaatctca acacgcttga tcagcgccgg atcgagaaaa gtaccattca
     3241 ggtgtccggt atccgttccc tgacgaacac catcgacaag aaccagcacg ccgcgatgat
     3301 catagccacg catatttaca tcctgaccgt tggttcgtcc ggtaccatcc agagtaattc
     3361 caggaacatg acgcagcaga tcggtggctg aagtagccgt ttgattttca ggagcggaag
     3421 tgtcgatgac gctgaccatc ataggcgctt cgaaggaact acgggcattc cccgttgccg
     3481 taacggtcat ggtttcagta gcaaaagcaa acgttggcaa ggtggcagaa acagccaaag
     3541 ccaacaaact caaacgcaac gaggtaaatt gcggacgtga catagcgatt ctccatgagg
     3601 aaaataaaaa atgctggctg cctgggttaa taccggggtg gctggcgaaa aataaattta
     3661 tttaaaattc aaagcgataa tattcgccgg gtgagtttac aggcgtttat atcccatgaa
     3721 attgcctggt agctcggcaa ttacgcaaaa gcacgccctc tttgtcaggc aaaacgatat
     3781 gccccatcag gagtgaagag cgctgtgcgt tatcccttgc aaatg
//
LOCUS       DAPGOF010000276         3643 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.281, whole genome shotgun sequence.
ACCESSION   DAPGOF010000276 DAPGOF010000000
VERSION     DAPGOF010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.281"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            167..1513
                     /locus_tag="RHV13_004555"
     CDS             167..1513
                     /locus_tag="RHV13_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001632353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243993.1"
                     /translation="MPMILVKKNPRGKVIRELSSEEETAVKTVCGLKRPATMAQHNLA
                     NDLLREMREYDAWLQCDCIPGDSPAMNFAALKNNTGTLYLSSFNHEHAPECPMYRQLS
                     GNEEETSFGASRHPVSTRINYRNFLPPDDSNSVIRLQARSAYHNGERSSVRKKRPRLG
                     RLLLSLIEDAGLNKLDSLANPRIRTNYRECLDAIRQVTLQQEYIRGRALSEIIHFRPG
                     MSERSQERLMETLENSERHWPARRKHMFFQIFMAQHICRDAVEIHWANGNIQVIRPVR
                     GISINGEAQGGIRPPYWVILAFCRSADGRIICSEGYAHALYQLTCPVPVDSKLERNTL
                     TALLNVASWLKRKPGTPELSLERPLFDTEVYVNGEKKYVLPDFIVTARAPDGKTARVV
                     IETMGYEDSDYCARKSRQHTGMKQIGVLHTDPPKWLDNEHPPFKKHMYGVFMHLRY"
     gene            1792..>3643
                     /locus_tag="RHV13_004556"
     CDS             1792..>3643
                     /locus_tag="RHV13_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin"
                     /protein_id="HDV1243994.1"
                     /translation="MSDPVRITNPGAESLGYDSDGHEIMAVDIYVNPPRVDVFHGTPP
                     AWSSFGNKTIWGGNEWVDDSPTRSDIEKRDKEITAYKNTLSAQQKENENKRTEAGKRL
                     SAAIAAREKDENTLKTLRAGNADAADITRQEFRLLQAELREYGFRTEIAGYDALRLHT
                     ESRMLFADADSLRISPREARSLIEQAEKRQKDAQNADKKAADMLAEYERRKGILDTRL
                     SELEKNGGAALAVLDAQQARLLGQQTRNDRAISEARNKLSSVTESLNTARNALTRAEQ
                     QLTQQKNTPDGKTIVSPEKFPGRSSTNHSIVVSGDPRFAGTIKITTSAVIDNRANLNY
                     LLTHSGLDYKRNILNDRNPVVTEDVEGDKKIYNAEVAEWDKLRQRLLDARNKITSAES
                     AVNSARNNLSARTNEQKHANDALNALLKEKENIRNQLAGINQKIAEEKRKQDELKATK
                     DAINFTTEFLKSVSEKYGAKAEQLAREMAGQAKGKKIRNVEEALKTYEKYRADINKKI
                     NAKDRAAIAAALESVKLSDISSNLNRFSRGLGYAGKFTSLADWITEFGKAVRTENWRP
                     LFVKTETIIAGNAATALVALVFSILTGSALGIIGYGLLMAVTGALIDESLV"
BASE COUNT         1060 a          782 c          909 g          892 t
ORIGIN      
        1 tattctctca ctttaatgtt gtcatgatat aaataaaaac aattcatttg aaaggatatc
       61 agaagaagat atcggggcgc acagttcatc tctgcctttg tttcatgggg ggttctttgt
      121 atattttact tactgtttaa gcatgtacat aaggagtgct ttcattatgc caatgatact
      181 tgtaaagaag aatccccggg gcaaagtgat tcgggagctt agcagtgagg aagagactgc
      241 agtaaagacg gtctgtggac tgaaaaggcc agcaaccatg gcacaacata acctggccaa
      301 tgatcttctc cgggaaatgc gtgagtatga tgcatggtta cagtgtgact gtattccggg
      361 agattcgccg gccatgaatt ttgctgctct taaaaacaat acgggtactc tttatctgtc
      421 cagttttaat catgagcatg cacctgaatg ccccatgtat cgtcagttat ccggaaatga
      481 agaggaaaca tccttcggag caagccggca tcccgttagc acccgtataa attacagaaa
      541 ctttttacca cctgatgaca gtaattctgt tatcagactg caggcccgct ctgcttacca
      601 taatggtgag cgctcctccg ttcgcaagaa acgtccgcgc cttggccgtt tactcctgtc
      661 gcttattgaa gatgcagggc tgaataaact ggattccctg gctaatcccc gtatccggac
      721 taattatcgt gagtgtcttg atgccatacg gcaggttaca ctacagcagg agtatattcg
      781 gggaagagcc ttatctgaaa ttatccattt ccggccagga atgtctgaaa ggtctcagga
      841 gcggttaatg gaaacgctgg agaattcaga acgacactgg cctgccagaa gaaagcatat
      901 gttctttcag atttttatgg cgcagcacat ctgtcgtgat gctgtggaaa ttcactgggc
      961 gaacggtaat attcaggtca tcagacctgt gcgggggatc agtattaatg gtgaagcgca
     1021 gggcggaata cgtccccctt actgggttat tcttgctttt tgccggagtg ccgatggcag
     1081 aatcatctgc agtgaagggt atgctcatgc tctttatcaa ctgacatgtc cggtgcctgt
     1141 ggacagcaaa ctggaacgaa acacgctcac tgctcttctg aatgtggcca gctggcttaa
     1201 aagaaagcca ggtacgccgg aattaagtct ggaaaggccc ctgtttgata cagaagttta
     1261 tgttaatggt gaaaagaaat atgtactgcc ggatttcatt gtcacagcaa gggctcctga
     1321 cggaaagacg gccagagtgg tcatcgaaac gatgggatat gaagacagtg attactgcgc
     1381 gagaaaatcc aggcagcata ccggcatgaa gcagattggt gttctgcata ccgatccacc
     1441 gaaatggctg gataacgagc atcccccttt taagaaacat atgtacggtg tttttatgca
     1501 tctcaggtac tgagatattt tgtggctcag ttctgtaact tttcccgtaa cattgtctgt
     1561 tgttacggga aagtccggtt tttgtattgc accagagaat acccagactg tgatgctgcc
     1621 acagcgtcag caggctttct gaacggtgta acatcacttc atgttaatga taatcactat
     1681 catcaaatct tgacatgcca ttttctcctt aataaattaa tactgtatat gtatccatat
     1741 gcgtaagcag ttaattcatt tgttttcctc agaggatgaa ggagataccg aatgtctgac
     1801 cctgtacgta ttacaaatcc cggtgcagaa tcgctggggt atgattcaga tggccatgaa
     1861 attatggccg ttgatattta tgtaaaccct ccacgtgtcg atgtctttca tggtaccccg
     1921 cctgcatgga gttccttcgg gaacaaaacc atctggggcg gaaacgagtg ggttgatgat
     1981 tccccaaccc gaagtgatat cgaaaaaagg gacaaggaaa tcacagcgta caaaaacacg
     2041 ctcagcgcgc agcagaaaga gaatgagaat aagcgtactg aagccggaaa acgcctctct
     2101 gcggcgattg ctgcaaggga aaaagatgaa aacacactga aaacactccg tgccggaaac
     2161 gcagatgccg ctgatattac acgacaggag ttcagactcc tgcaggcaga gctgagagaa
     2221 tacggattcc gtactgaaat cgccggatat gatgccctcc ggctgcatac agagagccgg
     2281 atgctctttg ctgatgctga ttctcttcgt atatctcccc gggaggccag gtcgttaatc
     2341 gaacaggctg aaaaacggca gaaggatgcg cagaacgcag acaagaaggc cgctgatatg
     2401 cttgctgaat acgagcgcag aaaaggtatt ctggacacgc ggttgtcaga gctggaaaaa
     2461 aatggcgggg cagcccttgc cgttcttgat gcacaacagg cccgtctgct cgggcagcag
     2521 acacggaatg acagggccat ttcagaggcc cggaataaac tcagttcagt gacggaatcg
     2581 cttaacacgg cccgtaatgc attaaccaga gctgaacaac agctgacgca acagaaaaac
     2641 acgcctgacg gcaaaacgat agtttcccct gaaaaattcc cggggcgttc atcaacaaat
     2701 cattctattg ttgtgagcgg tgatccgaga tttgccggta cgataaaaat cacaaccagc
     2761 gcagtcatcg ataaccgtgc aaacctgaat tatcttctga cccattccgg tctggactat
     2821 aaacgcaata ttctgaatga ccggaatccg gtggtgacag aggatgtgga aggtgacaag
     2881 aaaatttata atgctgaagt tgctgaatgg gataagttac ggcaacgatt gcttgatgcc
     2941 agaaataaaa tcacctctgc tgaatctgcg gtaaattcgg cgagaaataa cctcagtgcc
     3001 agaacaaatg agcaaaagca tgcaaatgac gctcttaatg ccctgttgaa ggaaaaagag
     3061 aatatccgta accagcttgc cggcatcaat cagaagatag cggaagagaa aagaaaacag
     3121 gatgaactga aggcaacgaa agacgcaatt aatttcacaa cagagttcct gaaatcagtt
     3181 tcagaaaaat atggtgcaaa agctgagcag ttagccagag agatggccgg gcaggctaaa
     3241 gggaagaaaa tacgtaatgt tgaagaggca ttaaaaacgt atgaaaagta ccgggctgac
     3301 attaacaaaa aaattaatgc aaaagatcgt gcagcgattg ccgcagccct tgagtctgtg
     3361 aagctgtctg atatatcgtc taatctgaac agattcagtc ggggactggg atatgcagga
     3421 aaatttacaa gtcttgctga ctggatcact gagtttggta aggctgtccg gacagagaac
     3481 tggcgtcctc tttttgttaa aacagaaacc atcatagcag gcaatgccgc aacggctctt
     3541 gtggcactgg tcttcagtat tcttaccgga agcgctttag gcattatcgg gtatggttta
     3601 ctgatggctg tcaccggtgc gctgattgat gaatcgcttg tgg
//
LOCUS       DAPGOF010000277         3635 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.282, whole genome shotgun sequence.
ACCESSION   DAPGOF010000277 DAPGOF010000000
VERSION     DAPGOF010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.282"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..147
                     /locus_tag="RHV13_004557"
     CDS             <1..147
                     /locus_tag="RHV13_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1243995.1"
                     /translation="LIHVKNIIPIITVTLILNYLNNSERSLVKQILIEDEIIVCATYL
                     IPDI"
     gene            149..493
                     /locus_tag="RHV13_004558"
     CDS             149..493
                     /locus_tag="RHV13_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HDV1243996.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            497..832
                     /locus_tag="RHV13_004559"
     CDS             497..832
                     /locus_tag="RHV13_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HDV1243997.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(888..3209)
                     /locus_tag="RHV13_004560"
     CDS             complement(888..3209)
                     /locus_tag="RHV13_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDV1243998.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTNGNLNTDTIIRVNPDGSAPPSFS
                     DGGNSFIVTAGNHAVGVLACASPGSARACVSSLDEESTADTGSNENSAKAILDMAKGE
                     ITTHGTESYAAYANGTVVKADGTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTFAVAHQGAGVVLESSINGQEATVDILSGSSL
                     RSANEILYNKNETSNVTITDSEVSSAADVFINNIKGHLTVYATDSKITGSANLSTDDN
                     THTYLSLSDNSTWDIKADSTVSKLTVDNSTVYIYRADGKDFEPTRLTITENYVGNNGV
                     LHLRTELGDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGADLLTGGFTDSDSWRLGVMAGYARDYNSTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDLSKKGAYIDAWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGARNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
BASE COUNT          955 a          878 c          801 g         1001 t
ORIGIN      
        1 ctcattcacg taaaaaacat aattcctatc atcaccgtca cgctcatttt gaattatctg
       61 aataatagcg agcgttcttt agttaagcag atcttaattg aagacgaaat tattgtgtgt
      121 gcaacttact taataccgga catctaagtt gaagaaaaaa atacttgcct tcgggcttat
      181 ctcagcattg ttctgctcca cgccagcgat ggcggatatg aaccgcacca cgaaaggtgc
      241 attgctaggc gcaggcgttg ggttacttac tggcaatggt gttaatggcg tactgaaagg
      301 tgcagctgtt ggcgctggtg ttggtgcagt aacagaaaaa ggccgcgacg gtaaaaatgc
      361 gcgtaaaggt gcaaaagtgg gtgccgccgt tggtgctgtc actggcgttc tgaccggcaa
      421 tggcctcgaa ggggcgatta aaggtgccgt gattggcggt acgggtggtg caattctggg
      481 taaaatgaaa taaggaatga tgatgaaaaa atttgcttta ctggcgggcc tgtttgtttt
      541 cgcaccaatg acctgggcgc aggactacaa tattaaaaac ggtttgccgt cagaaaccta
      601 tattacttgc gcagaagcca acgaaatggc aaaaactgac agcgcacagg ttgcagaaat
      661 tgttgcggta atgggtaatg ccagcgttgc cagccgtgat ttaaaaattg agcaatctcc
      721 ggaactgagc gccaaagtgg tagaaaaact gaatcaggtt tgcgctaaag acccgcaaat
      781 gctgttgatc acggcaattg atgacaccat gcgtgctatc ggcaaaaaat aatatttcat
      841 atgttaccgc gtcgatgaat tcgacgcggt attttagcta acggggatca gaacttatat
      901 ttcaggccca ccatcactgc ggtgtcattg tagccattat cacccagctg cacgcccaca
      961 ttaccccaga cactggccgc cggattcaga ttgccattta cgccaagttt aatttccccc
     1021 aaattacgcg ccccgtcttg ttcgactttc acaccgttca ttgagacagc aaaatcttta
     1081 ctgttatgga tccagttcat ttcaacaaaa ggttcaaagt cgtcaccgtg cggaccgctg
     1141 tttttctcct gagtacgaat aaaggtgcgg aaaccaagtc gggtctggat gttgttattt
     1201 gcgtcattct caatacgtga gccattggct tccgtatgcg cattatgatc gacgcccatc
     1261 cagatagcct gtgcctgtgg ctggaagatc cacgtatatt tcgccgcttc cagaccaaag
     1321 gatttattca gggcaaagcc gtaacccgct tccagcgaga cggttgcgcc tttcgcgcta
     1381 taggattcat aggctaattc atcccctttc accgagtttt taaaccagct atattgcgcc
     1441 caggcgtcaa tgtatgcgcc ttttttactg aggtcatcgg caaaccaggt ggcatacagc
     1501 cctgcgctat agcctctgac actccctttc gaacgataat ccgacacgct ggaatgagtt
     1561 gagttgtagt cgcgggcata accagccatc actcccaaac gccaactgtc actgtcggta
     1621 aaaccaccgg ttaacagatc ggcccctaac tgcgagacgt agcgatgcga ggtcgttctc
     1681 aactgtccgt tgctgtcacg ccaggcatta tgcccgccaa tttgacgtag ccaaagtcgg
     1741 cttgaacgct cctgttcagt tacaggatcg atgtaacgcg tttcacctgc acgatcgttc
     1801 agacgcataa caaaaagagt gttagcggct gccaggttgg cgacgtaact tccagcttcg
     1861 gggcgcagga cgggggtagg cgcaacagta ggcgctgaac ttcctccgga gtttgtttca
     1921 ccgccgctcg ttgcctggaa gttagtcaga taccagtttt tattggtcgc ttcggtatta
     1981 cctcgggtta atgaatattc gtaggcaccg gcgaaaatcc tcgaatcctt aataaattct
     2041 ccatttgatt ccccctcaac gctgataatc tctatcccat ttaacgtata agccccgctg
     2101 ccgccagcat tggtaacttt aactcgggtt gttccagagg tatttccatt aataacgact
     2161 ttatccgtag ctgaattatc atcacccaat tcagttctga gatgcaatac gccattatta
     2221 ccaacgtaat tttcagttat tgttaatctt gttggttcga aatcctttcc atctgcccgg
     2281 taaatataga ccgtactatt gtcaaccgtg agcttgctca ccgttgagtc agctttaata
     2341 tcccaggtac tattatctga aagcgacaga taggtatgag tgttatcatc tgttgaaaga
     2401 ttggctgaac ccgttatttt tgaatcagtt gcatagacgg tcaaatgacc tttaatatta
     2461 ttgataaaaa catctgcagc cgatgatacc tcactatcag taatggtcac gttactcgtc
     2521 tcatttttat tgtagaggat ttcatttgct gaccgtagtg agctaccaga taaaatatct
     2581 accgttgctt cctgaccatt tatggaagac tccagcacaa ctccagcccc ctgatgcgcg
     2641 actgcaaatg tatttttcag cgtcaccgtg ccgccattat aaattttagc aatcgcggcg
     2701 tcaggacccg ttgtataaat ttctccctgg ttaaatgaaa ctgtcccctg gcgagcagcg
     2761 atggcatgag cattgtcccc atgcgtggtg atatcaacat cggttaatgt aacgctggca
     2821 ttggtataat caagcgtgcc atcagccttg acgacagtgc cattagcgta agcggcataa
     2881 ctttctgttc cgtgggttgt tatctcccct tttgccatat ccagtattgc tttggcgcta
     2941 ttttcattac ttcctgtatc ggcagtactt tcttcatcaa gagaagatac acacgcacgc
     3001 gcgcttccgg gagatgcaca cgcaagaacg ccgactgcgt gattacccgc agtaacgata
     3061 aaagaattac ctccgtcaga aaaagagggg ggagccgaac cgtcagggtt gactcttatt
     3121 atggtatcag tattcaggtt tccattcgtt tgacttagca aaccaattga gtaatccccc
     3181 gttgtgacaa tcgtgttttt accctgcaaa ataacagcac cgccaaattc acttacgatt
     3241 gcgctagctg ttaatgctgt gcttagagaa ctattgtcag aaggatataa accgcccgtt
     3301 gttacaatga cgccattgtt aatggttaac ttgctaccct ctcctgcaac agataccccg
     3361 taacttccat tacccgttgt tataactagc gcattatcaa tattttctgt atttgcacta
     3421 tctattttca gcgcatgttc cttatcttca tgctgaataa acggtccagc atatgcatta
     3481 cccaccgcca gagcaataag tcctaaacta aacccgggga gttttttcag tttcatagat
     3541 taaccatcca ttcattggat taacaaacat tcctggtcaa ataacctatg agctcagatt
     3601 accaaaacgg agggtaaaga caaaaacaaa agcac
//
LOCUS       DAPGOF010000278         3529 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.283, whole genome shotgun sequence.
ACCESSION   DAPGOF010000278 DAPGOF010000000
VERSION     DAPGOF010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.283"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            306..434
                     /locus_tag="RHV13_004561"
     CDS             306..434
                     /locus_tag="RHV13_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HDV1243999.1"
                     /translation="MAYCFCEWHCSSRIMSVLADGVLVAGVVVIAFPLLKKKTPDC"
     gene            801..1229
                     /gene="tolA"
                     /locus_tag="RHV13_004562"
     CDS             801..1229
                     /gene="tolA"
                     /locus_tag="RHV13_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001479285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HDV1244000.1"
                     /translation="MGFRSASILTLIISGIIIGCTDAVSTNYHDRTSYYSDKAIETQY
                     VSSSERTSDVSEDIRLYAHQIKSAIEKQFGDASKYSGKECTLRMHMAPNGLLLEVKRE
                     SGDLDLCREAMNAIKNADIPAPPSPEVYKVFQNGVLDFKP"
     gene            1401..1775
                     /locus_tag="RHV13_004563"
     CDS             1401..1775
                     /locus_tag="RHV13_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tolA family protein"
                     /protein_id="HDV1244001.1"
                     /translation="MLTFWVGFVAGCTPLHPSDCHKTTATGSCSSGRWDDQDEWGAQA
                     RGIRAAINAKLDEPHNWKGKKCRLHMEFSQDGTALKISTSNGDKAYCEAIKSAAHKAK
                     FPAFNNPEVYRDFQKSGFDMRG"
     gene            2027..2242
                     /gene="essD"
                     /locus_tag="RHV13_004564"
     CDS             2027..2242
                     /gene="essD"
                     /locus_tag="RHV13_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HDV1244002.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            2247..2558
                     /locus_tag="RHV13_004565"
     CDS             2247..2558
                     /locus_tag="RHV13_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
                     /protein_id="HDV1244003.1"
                     /translation="MTQDYELVVKGVRNFENKVTVTVALQDKERFDGEIFGLDVAMDR
                     VEGAALEFYEAAARMSVRQVFLEVAEKLSEKVESYLQHQYSFKIENPANKHERPHHKY
                     L"
     gene            2555..3088
                     /locus_tag="RHV13_004566"
     CDS             2555..3088
                     /locus_tag="RHV13_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HDV1244004.1"
                     /translation="MNTKIRYGLSAAVLALIGAGASAPQILDQFLDEKEGNHTMAYRD
                     GSGIWTICRGATVVDGKNVFPNMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALTCWGIEQ"
     gene            3085..>3529
                     /locus_tag="RHV13_004567"
     CDS             3085..>3529
                     /locus_tag="RHV13_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
                     /protein_id="HDV1244005.1"
                     /translation="MNQIFTVILLVLVGFVVGNVWSDRGWQKKWAERDAAELSQEVNV
                     QFAARIIEQGRSISRDEAVKDAQQKAAEISARAADLSDSVNQLRAEATKYAIRLDAAQ
                     HTANLAAAVRGKTTKAAEGMLTNMLGDIAAEAQLYAEIADKRYIAG"
BASE COUNT          985 a          693 c          904 g          947 t
ORIGIN      
        1 gaggcgtcgg ggtgtttcat ccttaggcag cgtattgata tagtcaatgc agcacgagca
       61 aaggccttca gccgtttgac agttttgttt tgtactcctg atcgtctttc gggaagagac
      121 gttattattc tgaatagtga tgctatacag agggtttgcg atgagttcat ggttgctaat
      181 tcagaattat ttgctcttgt tcaggagtac aacagaatag ccaggacctg tggtatggat
      241 gaacttcgga ttactcatct ggggtagata catatctgga ttatcacctg ttacggtaaa
      301 aagttattgc ttactgtttt tgtgaatggc attgcagcag ccggataatg tcagtgctgg
      361 ctgacggtgt gctggtggcg ggtgtggtgg ttattgcttt cccgttgctg aaaaagaaaa
      421 cgccagactg ttagccgggt atcagttagc gggagaaatt tttaaatact tcacaattca
      481 ggcggttgac tgttgtctgg tttgcgggga gtttgttaaa agaaactggc atggtgaatc
      541 cccctgtgcg gaggggcaat cagcgagtag gtatatggga taatcgcgga ttcaggtgct
      601 ggtactgaat tcaccgggag gcacccggca ccatgcactt caatagattc tctccacatt
      661 atggatattc tttcagaata tcccacgcag actttgtgta aatgttaaca aatgtgcgtt
      721 ttatttgatc tgattcgctg tttgagcgtc caaaacaacg gtatatataa tcctttacta
      781 tatgacatat gtgaggaaaa atgggttttc gtagcgcatc aattcttacg ttgattatta
      841 gtgggattat tatcgggtgc actgatgctg tatcgacaaa ttatcatgac cgtacatcat
      901 attactccga taaagcaata gagacacagt atgtgagttc atctgaacgt acttctgatg
      961 ttagtgagga tatccgtctg tatgcccatc aaatcaagag cgccatcgaa aaacagttcg
     1021 gggatgcgag taagtattca ggaaaagagt gtacactgag aatgcatatg gccccgaatg
     1081 gccttctact ggaggttaaa agagaaagtg gagacctcga tttatgtcgt gaagcgatga
     1141 atgcgataaa gaatgctgat atacctgccc ccccttcgcc ggaagtatat aaagtatttc
     1201 aaaatggggt gctggatttt aaaccctgat atttattgtt ttgtaataaa cggtttcggc
     1261 ttaggtttgt tctgacacaa ctacggcact gagctaaatt tagcggatag tcaactctga
     1321 gccagtggcg gacgtaacaa ctactattgc tgagatttta atggattgag gagcaagaag
     1381 tgggattaaa gaaaatcgtt atgttgactt tttgggtcgg ttttgttgcg ggatgcacac
     1441 ctttacaccc ttcagattgc cacaaaacta ctgctacagg tagttgcagt tcaggacgct
     1501 gggatgatca ggatgaatgg ggggcgcaag cgcggggaat cagagctgca attaatgcca
     1561 aacttgatga gccgcataac tggaaaggga aaaaatgcag gttgcatatg gaattctctc
     1621 aggatggcac ggcgttaaaa atatctacca gtaacggtga taaagcctat tgcgaagcga
     1681 taaagtccgc agctcataaa gccaaatttc cggccttcaa caatccggaa gtctacagag
     1741 attttcagaa atctggcttt gacatgcgag gttagctctt caattactat atctcattca
     1801 tagcaaactg acagatttga tgatgttcta tatacgaaac ctgtgatgtc aagtctgagc
     1861 taatacaaat aaacataata tcagagaaat acattttatt agctcgctac ggcgagcttt
     1921 ttatattgca tcgtctccag catatatatc aattatggct ctgattgatg tgtctggaag
     1981 cctacacata ataactatgc catccgttcc gtgcggaggt gaggctatga aatccatgga
     2041 caaaatttca acaggcattg cctacggcac ctccgcaggc agtgctggct actggttttt
     2101 acagctgctc gataaagtca cgccctcaca gtgggcagca ataggtgtgc tgggtagcct
     2161 ggtatttggc ctgctgacgt acctgacaaa tctttatttc aagattaaag aagacaggcg
     2221 taaggctgcg agaggagagt aatccaatga ctcaagacta tgaactggtt gtgaaaggag
     2281 tccgtaattt tgagaataaa gttacggtaa ctgtagcctt acaggacaaa gaacgctttg
     2341 acggtgaaat ttttggcctg gatgtcgcca tggaccgtgt tgaaggagct gcgctggagt
     2401 tttatgaggc agcagccaga atgagcgtcc ggcaagtctt cctggaagta gcagaaaaat
     2461 tgtcagaaaa agttgagtct tatctgcagc atcagtactc ctttaagatt gaaaatcctg
     2521 ccaataagca cgagcgtcct catcataaat atctatgaac acaaaaatca gatacggcct
     2581 gtcggctgcc gttctggcgc tgattggtgc tggcgcatct gctcctcaga tacttgacca
     2641 gtttctggac gaaaaagaag gtaaccacac aatggcatac cgcgatggtt ctggcatatg
     2701 gaccatctgt cggggtgcca cagtggtgga tggaaaaaac gtttttccca atatgaaact
     2761 gtcgaaggaa aaatgcgacc aggtcaacgc cattgagcgt gataaggcgc tggcatgggt
     2821 ggagcgcaat ataaaagttc cactgaccga gccacagaaa gccggtatcg cgtcattctg
     2881 tccctataac attggccctg gtaagtgttt tccgtcgacg ttttataagc ggcttaatgc
     2941 cggtgatcgt aaaggcgcat gcgaggcgat tcgctggtgg ataaaggacg gtggacgtga
     3001 ttgccgcatt cgttcaaata attgttacgg tcaggttatt cgtcgtgacc aggagagtgc
     3061 attaacctgc tgggggatag aacagtgaat cagatattca cggtgatttt gctcgtgttg
     3121 gtaggatttg tcgtaggtaa tgtctggagc gacagaggat ggcaaaaaaa atgggcggag
     3181 cgtgatgctg ccgaattatc tcaagaggta aatgtccaat ttgctgctcg aataattgaa
     3241 caggggcgaa gtatatcccg tgatgaggct gttaaagatg cacaacagaa agccgctgaa
     3301 atttctgcca gggctgctga tctgtctgat agtgttaacc agctgcgtgc cgaagcaaca
     3361 aaatatgcca tacgccttga cgcagcgcag cataccgcaa atcttgccgc tgccgtcaga
     3421 ggcaaaacaa ccaaagccgc cgaaggaatg ctcaccaaca tgctcggaga tattgcagca
     3481 gaagctcagc tttatgctga aattgctgac aaacgctaca tcgcaggag
//
LOCUS       DAPGOF010000279         3399 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.284, whole genome shotgun sequence.
ACCESSION   DAPGOF010000279 DAPGOF010000000
VERSION     DAPGOF010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.284"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(44..502)
                     /locus_tag="RHV13_004568"
     CDS             complement(44..502)
                     /locus_tag="RHV13_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HDV1244006.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLERIYKLLVSTQ"
     gene            complement(557..1408)
                     /gene="manZ"
                     /locus_tag="RHV13_004569"
     CDS             complement(557..1408)
                     /gene="manZ"
                     /locus_tag="RHV13_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HDV1244007.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1421..2221)
                     /gene="manY"
                     /locus_tag="RHV13_004570"
     CDS             complement(1421..2221)
                     /gene="manY"
                     /locus_tag="RHV13_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HDV1244008.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2284..3255)
                     /gene="manX"
                     /locus_tag="RHV13_004571"
     CDS             complement(2284..3255)
                     /gene="manX"
                     /locus_tag="RHV13_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HDV1244009.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVPKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
BASE COUNT          910 a          864 c          808 g          817 t
ORIGIN      
        1 ttattaaaaa tacactaaac atagctttgg ctaaattaat aactcattga gttgaaacga
       61 gaagtttata aattctttcc agatcgtcga tatttcgaac gcggattaac agccgacgct
      121 gttctaattg catcaccaac acgccatctt ccgacaagtt catcgctttg attcggctat
      181 attcaatcca gacattggcg aagaaaaaac ctttttgttt aaagatgatc ttcggaacgc
      241 ggatccagaa tatataaaaa cccatcagag ccagtgcgct taataaccat gtcgttatta
      301 acgcaccatg attcgtgacg ttgttataga taagaatgac aatcaatccg acgaagataa
      361 cgctatcgat gcgaccacgc cggagcaaag gaattgccag cagggtgggg ccgttacggc
      421 ggggcatgat gaactgatcg tagatcgcga aggccaggag tgcggcgatg aaaagaatca
      481 gtaccaggtc cgtgattgtc attaatcctc cagataaaaa aacggggcca aaaggccccg
      541 gtagtgtaca acagtcttac agtcccagca ggccgcaagc gtaaccagcg ataccgatga
      601 cgaagaagcc aacgatgatc cacagcgggt taactttttt gcgcagtagc cacatacaag
      661 caaaggtcag aagcagtggt accaggcctg gcattaactg gtccagaata gtctggacag
      721 tagtaacgtg ttctttgccc gtctggtcag taatgcgaga gacaaccagc gggatgttga
      781 catgtgtcca cttgttaacc aatgccccca tgacaaacag gccgaggata gacgcccctt
      841 ccgtcagttt ttgcaggaag ccaccaccca tatctttaac gatatcgata cctttggagt
      901 aaccatacgc tacgccgtag taacgggttg ccagacgcac caggttaaac aggatgaaga
      961 acagcagcgg acctaacagg ctgccgctca tcgcgatacc ggcacccagt gctgcaaata
     1021 ccggacgtac ggttccccag aagatcgggt cgcctacacc agccagcggc cccatcaaac
     1081 cgactttgat accgttgata gcaccgtcgt cgatctctgc gccattagca cgctgttctt
     1141 ccagcgccag ggttacgccg agaatcggcg cagccacgaa cggctgggtg ttaaagaact
     1201 ccaggtgacg gcgaatagct tgtttacgag cttcgttgtt ctcagggtag aggcgacgaa
     1261 ttgccggtac catagagaag cagaaaccca gtgcctgcat acgttcgaag ttccatgaac
     1321 cctggaagag gttagaacgc aggaagacgc cacgaatatc actttgagtg agttttttct
     1381 cggtggtagt ttgagttgta tcaaccattt cgctcacctg ttagtccagt tcgttatcga
     1441 gatcgttgtt accagcagcc tgagcaggcg caccggctac gcggttgtat ttcgggctaa
     1501 gttggatgta gagcactgcc ataacagtac cgatcacacc cagagcaacc aggttaaagt
     1561 tggtgaatgc tgcggttacg aagccgaggt agaagaacgg catcaggtag ccagcacgca
     1621 tcatgttgat aaccattgca taaccaacca ccacgatcat gccaccggcg atattcagac
     1681 cattggtcac cacttccgga atcgcattca gcatgttctg tacttcgctg gtacctacag
     1741 acagcgcaac gatgacggcc ggaatagcca cacgcattgc ttgcaggaac agagaagaaa
     1801 cgtggatcca ggaaatcgct gtcaggttgc cgttatcagc agccttatcc gcagcgtgct
     1861 ggaaagcaac ggtaatagta cgaacgatga tggtcagtac ctggcccgca gcggccagag
     1921 ggattgccag tgcaatacct gcaccaatgc tctgatgacc tgcgataacc agaatggtag
     1981 aaatgataga tgccagagcg gcgtcaggcg caactgcagc accgatgttc atccagccca
     2041 gcgcgatcat ttccagcgta ccaccgataa taataccggt tttcatatcc ccaagaacga
     2101 tacccaccag ggtacacgcg attagcggac ggtgaaactg aaattcatcg aggattgatc
     2161 ccatacctgc gatacaggct acgataaata ccagcacaat ttgaagagtg gtaatctcca
     2221 ttgtacttct cctgtttacg taagactaaa gtgtgaaaac tgagtgataa tcaacacaat
     2281 acgttactta tcgattttgc tgatcagatc catcattttc agtttcggat cggtggaaac
     2341 cttacggact tccagctcaa taccgcgtgc attcagtttt ttgaacgcct cgatatcttt
     2401 ttcatcaacc gaaaccgcgt tattcacctg ggttttaccc tgacggaatg ccataccacc
     2461 gacgttaaca gaggtgattt tcacgccgcc ttcaacgaga cgctctacat ctgttgggtt
     2521 ggtaaataac agcattacac gttcgccagc atatttcggg ttgttgtaga cgcgaatcat
     2581 tttgggaaca tcaactacgt gtgctgttac gcccggaggt gcaacctggg tgagcagtgt
     2641 cttacgaaca gtatccgcag ccacttcatc actaacaaca ataatacggg agacattggt
     2701 ttctttggtc cagcgggtgg cgacctgacc gtgaatcaga cggtcgtcga tacgcgcaag
     2761 gccaataacc atgtagtcgt ttggccccat tggttttgcc ggagttggag ccgctttggg
     2821 tgctgcggca gcgggtgctg gcgcggcttt ttcaaccggt ttggctttca gtgctttcac
     2881 gccttcacgg cctgtttcta ctgccagcgc aaccagttca tcaaagcttg ggtcatcatc
     2941 acgagccatt aacgtttcca cgagcattgg aatgttaacg cctgcaatga cttcataatg
     3001 ctctttgtcg acgacaatgc ggctggcagc attgaacggg ctgcctcccc atgtatcaac
     3061 gagaaacagc acgcctttag tggtgtcgag ttttgccaac tgagcgttgt acttttcaat
     3121 cagcgtttcg gcattttcac ctggaacgaa atcgatccag ccgacgtttt cctgctcgcc
     3181 taacagcatt tctgccgttt taagcaactg ctctgcagcc caaccatgtg tgcctataac
     3241 aatagcaatg gtcacttgct acctccttta ttatcgttaa cacctcaacg tgccagatgt
     3301 atttttgaat cgtcgtccac aatcgaatcg attcagataa gggctacaac caaaaacccc
     3361 tgcgtttcgc gatttatttt agatatcgaa aaaattatt
//
LOCUS       DAPGOF010000280         3394 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.285, whole genome shotgun sequence.
ACCESSION   DAPGOF010000280 DAPGOF010000000
VERSION     DAPGOF010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.285"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            128..1093
                     /gene="dusB"
                     /locus_tag="RHV13_004572"
     CDS             128..1093
                     /gene="dusB"
                     /locus_tag="RHV13_004572"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDV1244010.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            1119..1415
                     /gene="fis"
                     /locus_tag="RHV13_004573"
     CDS             1119..1415
                     /gene="fis"
                     /locus_tag="RHV13_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HDV1244011.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            1501..2385
                     /gene="yhdJ"
                     /locus_tag="RHV13_004574"
     CDS             1501..2385
                     /gene="yhdJ"
                     /locus_tag="RHV13_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HDV1244012.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            2469..2648
                     /locus_tag="RHV13_004575"
     CDS             2469..2648
                     /locus_tag="RHV13_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HDV1244013.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(2651..3313)
                     /gene="acrS"
                     /locus_tag="RHV13_004576"
     CDS             complement(2651..3313)
                     /gene="acrS"
                     /locus_tag="RHV13_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HDV1244014.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
BASE COUNT          928 a          787 c          804 g          875 t
ORIGIN      
        1 cacgctcatt ttaaatgcaa ttctttgatc catctcagag gattggtcaa agtttggcct
       61 ttcatctcgt gcaaaaaatg cgtaatatac gccgccttgc agtcacagta tggtcatttc
      121 ttaactcatg cgcatcggac aatatcagct cagaaatcgc ctgatcgcag cgcccatggc
      181 tggcattaca gacagacctt ttcggacgtt gtgctacgag atgggagccg gattgacagt
      241 atccgagatg atgtcttcta acccacaggt ttgggaaagc gacaaatctc gtttacggat
      301 ggtgcacatt gatgaacccg gtattcgcac cgtgcaaatt gctggtagcg atccgaaaga
      361 aatggcagat gcagcacgta ttaacgtgga aagcggtgcc cagattattg atatcaatat
      421 gggttgcccg gctaaaaaag tgaatcgcaa gctcgcaggt tcagccctct tgcagtaccc
      481 ggatgtcgtt aaatcgatcc ttaccgaggt cgtcaatgca gtggacgttc ctgttaccct
      541 gaagattcgc accggctggg caccggaaca ccgtaactgc gaagagattg cccaactggc
      601 tgaagactgt ggcattcagg ctctgaccat tcatggccgt acacgcgcct gtttgttcaa
      661 tggagaagct gagtacgaca gtattcgggc agttaagcag aaagtttcca ttccggttat
      721 cgcgaatggc gacattactg acccgcttaa agccagagct gtgctcgact atacaggggc
      781 ggatgccctg atgataggcc gcgcagctca gggaagaccc tggatctttc gggaaatcca
      841 gcattatctg gacactgggg agttgctgcc cccgctgcct ttggcagagg ttaagcgctt
      901 gctttgcgcg cacgttcggg aactgcatga cttttatggt ccggcaaaag ggtaccgaat
      961 tgcacgtaaa cacgtttcct ggtatctcca ggaacacgct ccaaatgacc agtttcggcg
     1021 cacattcaac gccattgagg atgccagcga acagctggag gcgttggagg catacttcga
     1081 aaattttgcg taaacagaaa taaagagctg acagaactat gttcgaacaa cgcgtaaatt
     1141 ctgacgtact gaccgtttct accgttaact ctcaggatca ggtaacccaa aaacccctgc
     1201 gtgactcggt taaacaggca ctgaagaact attttgctca actgaatggt caggatgtga
     1261 atgacctcta tgagctggta ctggctgaag tagaacagcc cctgttggac atggtgatgc
     1321 aatacacccg tggtaaccag acccgtgctg cgctgatgat gggcatcaac cgtggtacgc
     1381 tgcgtaaaaa attgaaaaaa tacggcatga actaattcag gttagctaaa tgcttgatta
     1441 aaaaggcgct actcggcatg gggaagcgcc ttttttatag gtgtcacaaa gggagtgacc
     1501 atgagaacag gatgtgaacc gacccggttt ggtaatgaag ctaagaccat tattcacggt
     1561 gatgcccttg ccgaacttaa aaagctacct actgaaagcg tcgatctgat ctttgccgac
     1621 ccaccgtata acatcggtaa aaattttgat ggtctgatcg aagcctggaa agaagatctg
     1681 tttatcgact ggctgtttga agtgattgca gagtgccacc gcgttctgaa aaagcagggc
     1741 agcatgtaca ttatgaacag tacggaaaac atgcccttta tcgatctcca gtgccgcaag
     1801 ctttttacca tcaaaagtcg catcgtctgg tcatatgaca gttctggagt acaggcgaaa
     1861 aaacactacg gctccatgta cgaacccatc ctgatgatgg tgaaagacgc aaagaactac
     1921 acattcaacg gtgatgctat tctggtagaa gccaaaaccg gatcgcagcg cgcgttgatc
     1981 gattatcgca aaaatcctcc acagccatac aatcatcaaa aagtaccggg taacgtctgg
     2041 gattttccgc gcgtgcgtta tttgatggat gaatatgaga accacccgac gcaaaaaccg
     2101 gaagccttac tgaaacgcat tattctcgcc tcttccaacc caggcgatat cgttctcgac
     2161 ccgtttgccg gtagctttac taccggtgcc gtagccatcg ccagcggacg aaaattcatt
     2221 ggtattgaga tcaacagcga gtacatcaaa atggggcttc gacggctgga tgtcgcgtcg
     2281 cattactccg cggaagaact ggcgaaagtg aaaaaaagaa agaccggcaa cctgtcaaaa
     2341 cgaagccggt taagcgaagt tgaccccgat ctcattgcaa agtaaaggaa gtgtaagcct
     2401 ggttttcaga ttattcattt cgtgtatatt tcctgccaga cttggttaaa catgcacagg
     2461 ctcaggtaat gattcgcaag tattggtggc tcgtcgtttt cgctgtcttc gtttttctgt
     2521 ttgatacttt actgatgcag tggattgaac tgctggcaac agaaacagac aaatgccgca
     2581 atatgaactc agttaatcca ctaaagctgg ttaactgtga cgaactgaat tttcaggaca
     2641 gaatgtgaat ttacatgaca cttaattcat tcgtttgatg aattaatttc gttatgtttt
     2701 catctggcat gaacattctt aatacgttat cgaccagcgc gggtgcttgt ttataaagat
     2761 cataacccgc catattcatt aaccagtttt gaacaattcc gctgaaggca ccatcaataa
     2821 taatcatcac aacatctaaa tcgaggttat ttgctacaca accttgttgc tgacacgcct
     2881 gcaatacttc gcggagagtc tgcggattaa agcccatctt ttcgcgtatc actccctcgg
     2941 ccagcatctc atcattaaat tcacatttgt gatataagat tttcagcaac gcctgctggc
     3001 ggggaatttt ggcaatatat tgcaagccga caatcaattt ttcacgcaat tgttgaaacg
     3061 ggtcatgctc taatccagcc gtcaagtgtt cctggattaa ctcccgcaat gaaggctgtt
     3121 gcaaccacat ctcattaaac agttgggtct tgttctcgaa gtgccagtag atagcgccac
     3181 gcgtaacgtt agcggcatcg gcaatgtcgt tgagcgtcgt cttgcttacg ccatgttgcg
     3241 caaactgggc gatggcagtt tcaatcagtt cttgccgggt cttcagagct tcggctttgg
     3301 ttctttttgc catgattaat tattcaggaa ataaatatat tcgacacaga gtgagaaaat
     3361 agcgaaggtt aatctatcac ctaatgtgta ttta
//
LOCUS       DAPGOF010000281         3381 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.286, whole genome shotgun sequence.
ACCESSION   DAPGOF010000281 DAPGOF010000000
VERSION     DAPGOF010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.286"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            346..1662
                     /gene="shiA"
                     /locus_tag="RHV13_004577"
     CDS             346..1662
                     /gene="shiA"
                     /locus_tag="RHV13_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HDV1244015.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPENKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISIMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDSQRA"
     gene            1764..3218
                     /gene="amn"
                     /locus_tag="RHV13_004578"
     CDS             1764..3218
                     /gene="amn"
                     /locus_tag="RHV13_004578"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HDV1244016.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          787 a          824 c          811 g          959 t
ORIGIN      
        1 caatacgtct caacaatgat gctaaaaaca taactaacct cgcctcccta ctggttataa
       61 tgcaatgcag tctatcagaa tcatcagggt gcaattttgt gcatatgcgg acttttatgt
      121 ttcatatctc gaacctgtgg ggcctctgct taatccttaa acaacaccag caactcctgc
      181 gctttcatct tccagcgaat ttttcatgtt gctgctcatc agccataaaa tcatttgcag
      241 atgcgctctg tcgaggtagt ctcataaggt tcgtttatag atcgacagca atgtgagtta
      301 ccttttccat actaattata aaaagacagt acaaacagga tcattatgga ctccacgctc
      361 atctccactc gtcccgatga agggacgctt tcgttaagtc gcgcccgacg agctgcgtta
      421 ggcagtttcg ctggtgccgt cgtcgactgg tatgattttt tactctatgg cattaccgcc
      481 gcactggttt ttaatcgcga gtttttcccg caagtaagcc cggctatggg aacgctcgcc
      541 gcatttgcca cctttggcgt cggattcctt ttccgtccgc tcggcggtgt cattttcggt
      601 cactttggcg accgactggg acgtaagcgc atgttaatgc tgaccgtctg gatgatgggc
      661 atcgcgacag ccttgattgg tattcttcct tcattctcga ccattgggtg gtgggcacct
      721 attttgctgg tgacactgcg tgccattcag ggatttgccg tcggcggcga atggggaggc
      781 gcggcgttgc tttccgttga aagtgcgccg gaaaataaaa aagcctttta cagtagcggt
      841 gtacaagttg gctacggagt aggtttactg ctttcaaccg gactggtttc attgatcagt
      901 ataatgacga ctgacgaaca gtttttaagc tggggctggc gcattccttt cctgtttagc
      961 atcgtactgg tactgggagc attgtgggtg cgcaatggca tggaagagtc cgcggaattt
     1021 gaacaacagc aacataatca agctgccgcg aaaaaacgca tcccggttat cgaagcgctg
     1081 ttacgacatc ctggtgcttt cctgaagatt attgcgctac ggctgtgcga gttgctgacg
     1141 atgtacatcg ttaccgcctt tgcacttaat tattcaaccc agaatatggg gttaccgcgc
     1201 gaacttttcc ttaatattgg tttgctggta ggtggattaa gctgcctgac aattccctgt
     1261 tttgcctggc ttgccgatcg ttttggtcgc cgcagggttt atatcacagg tgcgttaatc
     1321 ggaacgttga gcgcatttcc tttctttatg gcgcttgaag cacaatctat tttctggata
     1381 gttttcttct ccataatgct ggcaaacatt gcgcatgaca tggtggtgtg tgtgcaacaa
     1441 ccgatgttta ccgaaatgtt tggtgccagt tatcgctata gtggcgctgg agtcggttat
     1501 caggttgcca gtgtggttgg cggtggattt acacctttta ttgccgctgc actcatcact
     1561 tactttgccg ggaactggca tagcgtcgcc atttatttgc tggctggatg cctgatttcc
     1621 gcaatgaccg ctttgttgat gaaagacagt caacgcgctt gatagcctgg cgaagatcat
     1681 ccgatcttcg ccttacactt ttgtttcaca tttctgtgac atactatcgg atgtgcggta
     1741 attgtatgga acaggagaca cacatgaata ataagggctc cggtctgacc ccagctcagg
     1801 cactggataa actcgacgcg ctgtatgagc aatctgtagt cgctttacgc aacgccattg
     1861 gcaaatatat tacaagtggc gaattacctg atgaaaacgc ccgcaaacaa ggtctttttg
     1921 tctatccatc actgaccgta acctgggatg gtagcacaac caatccccct aaaacgcgcg
     1981 catttggtcg ttttactcac gcaggcagct acaccaccac gattactcgc cctactctct
     2041 ttcgttcgta tctcaatgaa caacttacgt tgctgtatca ggattatggt gcgcatatct
     2101 cggtgcaacc ctcgcagcat gaaatccctt atccttatgt catcgatggc tctgaattga
     2161 cacttgatcg ctcaatgagc gctgggttaa ctcgctattt cccgacaaca gaactggcgc
     2221 aaattggcga tgaaactgca gacggcattt atcatccaac tgaattctcc ccgctatcgc
     2281 attttgatgc gcgccgcgtc gatttttccc tcgcacggtt gcgccattat accggtacgc
     2341 cagttgaaca ttttcagccg ttcgtcttgt ttaccaacta tacacgttat gtggatgaat
     2401 tcgttcgttg gggatgcagc cagatcctcg atcctgatag tccctacatt gccctttctt
     2461 gtgctggcgg gaactggatc accgccgaaa ccgaagcgcc agaagaagcc atttccgacc
     2521 ttgcatggaa aaaacaccag atgccagcat ggcatttaat taccgccgat ggtcagggta
     2581 ttactctggt gaatattggc gtgggaccgt caaatgctaa aaccatctgc gatcatctgg
     2641 cagtgctacg cccggatgtc tggttgatga ttggtcactg tggcggatta cgtgaaagtc
     2701 aggccattgg cgattatgta cttgcacacg cttatttacg cgatgaccac gttcttgatg
     2761 cggttctgcc gcccgatatt cctattccga gcattgctga agtgcaacgt gcgctttatg
     2821 acgccaccaa gctggtgagc ggcaggcccg gtgaggaagt caaacagcgg ctacgtactg
     2881 gtactgtggt aaccacagat gacaggaact gggaattacg ttactcagct tctgcacttc
     2941 gttttaactt aagccgggcc gtagcaattg atatggaaag tgcaaccatt gccgcgcaag
     3001 gatatcgttt ccgcgtgcca tacgggacac tactgtgtgt ttcagataaa ccgttgcatg
     3061 gcgagattaa acttcccggt caggctaacc gtttttatga aggcgctatt tccgaacacc
     3121 tacaaattgg cattcgggcg atcgatttgc tgcgcgcaga aggcgaccga ctgcattcac
     3181 gtaaattacg aacctttaat gagccgccgt tccgataata agttctgaac cttcttatca
     3241 aaaatagatg tgaaggatta agtaagacat ctggcaaata gcccgcaata aacgcgggct
     3301 gtttgcttcc ttgggcggat acgagtttta ttatcgtctt aatgatttcc acatattaaa
     3361 agcaagtatg ctttcaaaac a
//
LOCUS       DAPGOF010000282         3366 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.287, whole genome shotgun sequence.
ACCESSION   DAPGOF010000282 DAPGOF010000000
VERSION     DAPGOF010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.287"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(27..233)
                     /locus_tag="RHV13_004579"
     CDS             complement(27..233)
                     /locus_tag="RHV13_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1244017.1"
                     /translation="MVNGLLLFAVSVLVFLVIILMLHLRTASLLSECSEYRTKCDNLY
                     HLCQMQEKELEHLRALIDAHNIDY"
     gene            complement(477..2633)
                     /locus_tag="RHV13_004580"
     CDS             complement(477..2633)
                     /locus_tag="RHV13_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HDV1244018.1"
                     /translation="MKLFIAEKPAVANDIVKALGGNFTRHDGWFESDNTIVTNCFGHI
                     IESQPPENYNPEYKEWKIETLPLRLYPVKYQPVESAEKQVKTIIELIRRADVTEIIHA
                     GDPDDEGQLLVDEVLEYAGNTKPVKRVLINDNTLPAVKKALANPKNNRDFRGLYLKAL
                     ARSVADAIYGLSMTRAYTIPAKTKGYKGVLSVGRVQTPVLGLIVNRTRANKNHKSSFY
                     YTMTGHFQRGADVIRANWKPGEFAPLTDRKLLDKTWANGTATSLAGKPATVEAAATDD
                     KKTAAPLPFNLVRLQQYMNKKFKMTAQKTLDITQQLREKYKAITYNRSDCSYLSDEQF
                     SEAPQIIDALKSVFDQPMDIDTTRKSKAFNSAKVTAHTAIIPTVSVPDVNALSTDERN
                     VYLAIAQHYLVQFMPEKAYQEVSVAIQCGDESFYARARKTTDSGFEAFLGVENAGDDE
                     AEVENDDSAFDLLCKIRTGETVTTKEVVVNEKKTTPPPLFTEATLLAALVRVADFVTD
                     PVIKKLLKDKDRDKKDEHGGIGTPATRASILETLKKRNYITLEKGKLIPTDTGYALID
                     ALPDIAVNPDMTALWAEKQTLIENGEMTIEQFVDELYNDLIPMISNANSAEIKVSPSA
                     PSGQSQRLSSPCPSCGKQIVIRPKSYSCTGCEFKIWNEFSGKKITQAQAEKLIKSGKT
                     DLIKGFKKKSGGTYDAVLVLEDKKTGKLGFPARAKK"
     gene            complement(2638..3213)
                     /locus_tag="RHV13_004581"
     CDS             complement(2638..3213)
                     /locus_tag="RHV13_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDV1244019.1"
                     /translation="MQYALLDGFERKFLLDALEFGVLKDWKENPVKELPDIDESAHPF
                     HVCYGGYLLNPGVSDSDISRKIKDQTGFWLAAIDDTRMDCHSIAYYDIHTLPLISCGH
                     QKIVPFAALIKADECIISKISSYSGFAVTAFLRIKDQDIATNILNREGIFAFNGCERR
                     FRHPVSEDNWQQAVSEERAIRCAKRLIQCKG"
BASE COUNT          889 a          753 c          690 g         1034 t
ORIGIN      
        1 atttatccat ccccctaaag caacgtctaa taatctatgt tatgcgcatc aatgagtgca
       61 cgtagatgtt ctaactcttt ctcttgcatc tggcatagat gatagagatt atcacatttc
      121 gtgcgatatt cagagcattc agaaagaaga ctggctgttc tcaaatgtaa catcaggatt
      181 atcactaaaa aaactaatac actaacagcg aaaagcaaca aaccattcac cataaaatct
      241 gtactctcca cgccgagaaa cgaaacatga gaattatcat caaaataaac ggatatttca
      301 aaggataacg ccctccagag aaaaggaggg ctgtagaatc atttctcgat gagaaactca
      361 tcaagggaac gacctcctgc cagtgcttca gcaatcggtt ttggcgctcg cccacgacca
      421 gacttggcta ttaactgaag caattcctga cctgaaaagc catttctgct ttgctttcac
      481 ttcttagccc ttgccggaaa acccagcttc cctgttttct tatcctcaag gacaagaacc
      541 gcatcatacg ttccaccgct tttcttttta aatcccttaa tcaaatcggt ttttcctgat
      601 ttaataagtt tttcggcctg tgcctgggtg attttcttgc cagaaaactc attccagatt
      661 ttaaattcac atcctgtaca ggaatagctt tttggcctga taacaatctg tttaccacag
      721 gaaggacaag gagaggacag gcgttgtgac tgaccagacg gcgcgctggg ggaaactttt
      781 atttcagcac tgttcgcatt actgatcatc ggtatcagat cattgtacag ttcgtcaacg
      841 aactgttcga tggtcatttc tccgttttca atgagtgtct gtttttccgc ccataatgcc
      901 gtcatatctg gattaacagc tatatccggc agagcatcaa taagcgcata cccggtatct
      961 gtcggaataa gttttccttt ttccagggtg atatagttcc gttttttcag tgtttccaat
     1021 atggaagccc gcgtagctgg cgtaccaata ccgccatgtt cgtctttttt atcccgatct
     1081 ttatccttca gtaacttttt aatcactgga tcagtgacaa aatctgcgac acgaacaagc
     1141 gcggcaagta aagtggcttc ggtgaataat ggcggtggcg ttgttttctt ctcattcaca
     1201 accacttctt ttgtcgtcac tgtttctcct gtgcgaattt tacagagaag atcaaaagcg
     1261 gaatcatcat tttcgacttc tgcttcatcg tccccggcat tttccacacc aagaaacgct
     1321 tcaaatccgc tgtctgtagt tttcctggca cgggcataaa acgactcatc accacactga
     1381 atggcgacgg atacttcctg atatgccttt tcaggcatga actggacaag atagtgttgt
     1441 gcaattgcca ggtaaacgtt gcgttcgtcg gtgctcagtg cgtttacgtc cggtacgcta
     1501 acggttggga tgatggctgt atgcgctgtt acttttgcgc tgttaaatgc cttgctttta
     1561 cgtgtcgtat caatatccat tggctgatca aacaccgact tcagggcatc gattatttgt
     1621 ggtgcttcac tgaattgttc gtctgaaaga tatgaacaat ctgaacggtt gtaagtaatt
     1681 gctttgtatt tttcgcgtag ctgctgtgtg atatccagcg ttttttgagc agtcattttg
     1741 aattttttgt tcatgtactg ctgaagtcgt accaggttaa agggcaacgg tgcagccgtt
     1801 tttttatcat ctgttgctgc cgcttcaact gtggctggtt ttcctgcaag tgatgttgct
     1861 gttccattcg cccaggtttt atcaagcaat ttgcggtctg tcagcggtgc gaactcacca
     1921 ggtttccagt tcgccctgat aacatcagcg ccgcgttgaa aatgtccggt catggtgtag
     1981 tagaagctgg atttatgatt tttattggca cgggtgcgat ttacaatcag gccaagcacg
     2041 ggtgtctgga cccgtccgac agacaaaaca cctttatagc ctttagtttt tgccgggatt
     2101 gtgtaagcac gcgtcatgga taaaccgtat atcgcgtcgg ctacagaacg cgccagagcc
     2161 ttgagataga gtcctctgaa gtcacggtta tttttgggat ttgccagcgc ctttttcaca
     2221 gccggaaggg tgttgtcgtt gatcagaacg cgcttaacgg gttttgtgtt gcctgcatat
     2281 tccaggacct catcgacaag tagctgtcct tcgtcatcag gatcgccagc atgaataatt
     2341 tcggtcacat cagcacgcct gataagctca ataatcgttt taacctgttt ttcggcgctt
     2401 tctacaggct ggtattttac gggataaaga cgcaaaggga gtgtttcaat cttccactct
     2461 ttgtattcag gattatagtt ttcggggggc tgtgattcga taatatgacc aaaacagtta
     2521 gtcacaatag tattatcact ttcaaaccag ccatcatgac gggtaaaatt gccaccaagc
     2581 gccttaacaa tatcattcgc tactgcgggt ttttctgcga taaaaagttt cattttgtta
     2641 tcctttacat tgaattaatc ttttggcaca acggatagca cgttcttctg atactgcctg
     2701 ttgccagtta tcttcactta ctggatgtct gaatctgcgt tcacagccat taaaagcaaa
     2761 aatcccctca cggttaagta tattagttgc aatatcctgg tctttaattc tcaaaaaggc
     2821 tgttacggca aaaccagaat acgaagaaat ttttgaaatg atgcattcat cagcttttat
     2881 taacgcggca aaaggaacta tcttttgatg accacacgaa atcagaggga gtgtgtgaat
     2941 atcataataa gctattgaat gacaatccat gcgggtatca tcaatagctg ccagccagaa
     3001 tcctgtctgg tcttttattt ttctgctaat atctgaatct gaaacaccag gatttaataa
     3061 atatccacca taacagacat ggaaggggtg agcagattca tcaatatcag gaagttcttt
     3121 taccggattt tctttccagt ccttcagaac accgaattca agagcatcca gcaaaaactt
     3181 tcgttcaaat ccatcaagca atgcatattg catgttaaac ctctcaaaga agctctgatt
     3241 tgttgacttc atggtgaacc agttcaccat tgcgtaagac aaacaggtaa gaaaactttc
     3301 tttccttatc tttcagtcag tttttgtatt cagaaagaat aaacacccag tcatcggtgc
     3361 tgtaat
//
LOCUS       DAPGOF010000283         3360 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.288, whole genome shotgun sequence.
ACCESSION   DAPGOF010000283 DAPGOF010000000
VERSION     DAPGOF010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.288"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(144..473)
                     /locus_tag="RHV13_004582"
     CDS             complement(144..473)
                     /locus_tag="RHV13_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000855123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1244020.1"
                     /translation="MKTLTFNNGTVSVGDVFVSSWGYEQTNVNFYQVISVHGKKTVTV
                     QEIRASVHRTHSMSGYKTPLLNDFCGEPLKRRVRDYYSTPAIEIESFETAYKGSPEEK
                     HEFTSYY"
     gene            1070..1735
                     /locus_tag="RHV13_004583"
     CDS             1070..1735
                     /locus_tag="RHV13_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HDV1244021.1"
                     /translation="MGRALLVASDKGGVGKSTTATNLASYLVNNSRTVIILKTDKNND
                     ILSWNEKRKQNGLLPVPVYEEYGDVSEVIKRLKKICEVLIIDCPGHDSKEFRSALTVA
                     DILLTLVKPSSDFEAETLTHVTEKVRTAQQINPDLQPWVLFTRINTTKPRHRKAAIDL
                     DKLLRENPVWIQPLRTRISDLDIFEAACNEGAGVHDVRRASSLSTAKAQIELLAQELG
                     INP"
     gene            1809..2093
                     /locus_tag="RHV13_004584"
     CDS             1809..2093
                     /locus_tag="RHV13_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HDV1244022.1"
                     /translation="MALDLKTPVIPGQKTEQTNADTARFINEATRKKSGATKVTNVRL
                     GDVYEDILEREALRTGQTKTAIMKAALAAYEHGLDENGKNHWILQSARLK"
     gene            complement(2111..2707)
                     /locus_tag="RHV13_004585"
     CDS             complement(2111..2707)
                     /locus_tag="RHV13_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1244023.1"
                     /translation="MTAIIEASMQPKKTPVIVVKKRRVLVMPENPVVNEKPQEVQKSA
                     VNENKKVQKKDAVAEKTHKKQPQPWYLKKQITFPPKYPKEYFEKCFNKVRAVFPELWT
                     DEKKNLPLKSGILQDVEKYLADNPDVDLTIEEWNCAVQVMTFRWQYLQNCTVPGATRY
                     DLYGKPAGTVKKAHATYAQLVLDARKKASEKKQLKRKG"
     gene            complement(2857..3018)
                     /locus_tag="RHV13_004586"
     CDS             complement(2857..3018)
                     /locus_tag="RHV13_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HDV1244024.1"
                     /translation="MKQPKNALTWCLLIVCCTLLIFTYLTRNRLCEVRLKDGNREVTA
                     SLAYESNGK"
BASE COUNT          948 a          799 c          722 g          891 t
ORIGIN      
        1 tacattgctg cattcgcctg gcattctcca cgccttgaag gacggagttt ttgcgcacca
       61 aataaaaaag gggcgattaa gcccctgtat tcactattaa caatttaaca tggttgcagg
      121 ggataccccc tgcaactatt ttcttagtag tatgatgtga actcgtgttt ttcttccggt
      181 gatcctttat atgctgtttc gaatgactca atttcaattg ctggcgtact gtaataatca
      241 cgcactcgcc gttttaatgg ctcaccacag aaatcattga gtaatggggt cttatatccg
      301 ctcattgaat gggtccgatg aaccgaagcg cgaatctcct gaacagttac ggtttttttg
      361 ccatgaacag aaataacctg atagaaatta acgttcgttt gctcatatcc ccaggaagat
      421 acaaacacat cgccaacaga aacagtgccg ttattaaaag ttaatgtttt catttttatc
      481 tcctgccgct gagcggcttt ggtggttggg gaccatcccc tcccgatgtg tttataatag
      541 gtcagaactg acctattatc aagtattttt ccctccattc ttcaaaaaaa acaccatcaa
      601 atacatatag ttacattcat gtgatgcccg acagggcatg gaaggggcga aagaaaatac
      661 cagggagcag cggggccaca tcgccccgaa gcgcggcgca gccgctgacg aacataccag
      721 gatgcacagg gccatccctt caccctgcaa agcccaacgg actgacaggc catgatcatg
      781 ctgatttgcg ccgcagaatg ccgcagttag cgcgagcgcc agcgagttta cgccgtcctg
      841 gtgcattgca gcagggcggg gcgctgtact gcgcgtgtga gcgtcagcga gctacgggcg
      901 caacaacaca ccaggcaaaa tcgcatcctg aaaagttacc ctgtaacata cacacaatgt
      961 aaagcacaaa agcaattaca ttgtaaagac acaaacatct ttacaacgta actataatga
     1021 atacaatgta aagacaaata aacctttaca ctgtatatcc gaggtttcta tggggagagc
     1081 attacttgtc gcatcagata aaggcggtgt aggaaaaagc accacagcaa caaacctggc
     1141 ttcttatctg gtaaataatt cacgaacggt catcattctg aagaccgata aaaacaatga
     1201 tattcttagc tggaacgaaa aaagaaagca gaatggctta ttaccggttc ctgtttatga
     1261 agagtatggc gatgtatcag aagtcattaa gcgcctgaaa aaaatatgtg aagtgctgat
     1321 cattgactgt ccagggcatg acagcaagga atttcgcagt gccttaactg tagctgacat
     1381 tctgcttact ctcgttaagc cgtcttccga tttcgaagca gaaacgctga ctcatgtaac
     1441 agaaaaagtt cgcacagctc aacagatcaa tccagacctg caaccctggg tacttttcac
     1501 gagaattaac acgacaaaac cacgccacag aaaagcagcg attgatctcg acaagttact
     1561 ccgggagaac ccggtgtgga tacagccact tcgtacacgt atttcagatt tagatatttt
     1621 tgaagcggcc tgtaatgaag gcgctggcgt tcatgatgta cgtcgcgcat ccagcctttc
     1681 tacagctaaa gcacaaatag agcttttggc gcaagagtta ggcatcaatc cctaatggat
     1741 gtaatacgat agctttacat cattaacttt acacagtaaa gctaatgtaa cacacgaaag
     1801 gtgatataat ggctcttgac ttgaaaactc cggtaatacc aggacaaaaa acagaacaaa
     1861 ccaatgcaga cacagcccga ttcatcaatg aagctacccg caaaaaatcg ggagcaacta
     1921 aagtgaccaa tgtccggctt ggggacgttt acgaagatat tctggaacgg gaagcactgc
     1981 gaacaggcca gacaaaaaca gcaataatga aagcggctct tgcagcttac gaacacggcc
     2041 tggacgaaaa cggaaaaaat cactggattc tacagtcagc caggctgaaa tgataaagcc
     2101 cttcggggct ttatccctta cgttttaatt gtttcttctc gctggctttc ttacgggcat
     2161 cgagaaccag ttgcgcataa gttgcgtgtg ctttttttac tgtaccagca ggtttcccgt
     2221 aaaggtcgta acgtgttgcg cctggtacag tacaattctg aagatattgc caccggaacg
     2281 tcatcacctg aaccgcacaa ttccactctt caatcgtcag atccacatcc gggttatccg
     2341 ccaggtattt ctcaacgtcc tggagaatcc cgcttttcag gggcaagttc tttttttcgt
     2401 ctgtccatag ttcaggaaaa acagcacgaa ctttattgaa acacttttca aaatattctt
     2461 tcggatattt tgggggaaaa gtgatttgtt ttttcaaata ccagggctga ggctgctttt
     2521 tatgtgtttt ctctgctaca gcatctttct tctgtacttt cttgttttca ttaactgctg
     2581 atttttgtac ttcctgcggc ttttcattca ccacaggatt ttctggcatg accagaacgc
     2641 ggcgtttttt gaccacaatc acgggtgttt tttttggctg catactcgcc tcaataattg
     2701 ctgtcatatt gactaattat accattttca gcagttaata tcctgaaaat acatggaaaa
     2761 agggggtatc cgtacagaat accccctttg caaaggcgct taatgccaga accacatcat
     2821 ccccaaaaag gcgggctttc gcccgcctcc gggttgctac ttaccgttgg attcgtaagc
     2881 cagacttgcc gtaacctccc tgtttccatc tttcagccgg acctcgcaca aacgattccg
     2941 ggtcagatac gtgaatatta acaatgtgca gcacacgatt aataaacacc aggtcagggc
     3001 gtttttcggc tgtttcatgg ccttcttctc cttgcgtaac gcgcggtaag aggctatcct
     3061 gatgttgttg aagcattggg atcgcctcgt gggttaatga aaaattaact acggggcttt
     3121 cttctttctg ccgcacaaca agatacaaac caccttttcg ccatgaggca gaaagcctca
     3181 agcgccaaca gcattatata ttgacctgga attaaattcc cagctttcgg cgtggactat
     3241 acagtgtgtg attcaccacg atgaatcctt tgttgatcca ctgtgtgacc tgcgcaggtt
     3301 gaactccggt cagcctggcg aacgatgcct gattcccccg atagaacttg ttgatgtatt
//
LOCUS       DAPGOF010000284         3291 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.289, whole genome shotgun sequence.
ACCESSION   DAPGOF010000284 DAPGOF010000000
VERSION     DAPGOF010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.289"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            155..1675
                     /locus_tag="RHV13_004587"
     CDS             155..1675
                     /locus_tag="RHV13_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HDV1244025.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHTLERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSTTVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEGT
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(1700..2038)
                     /gene="maoP"
                     /locus_tag="RHV13_004588"
     CDS             complement(1700..2038)
                     /gene="maoP"
                     /locus_tag="RHV13_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HDV1244026.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            2157..2996
                     /gene="hdfR"
                     /locus_tag="RHV13_004589"
     CDS             2157..2996
                     /gene="hdfR"
                     /locus_tag="RHV13_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HDV1244027.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(3092..3167)
                     /locus_tag="RHV13_004590"
     tRNA            complement(3092..3167)
                     /locus_tag="RHV13_004590"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3132..3134),aa:Trp,seq:cca)
     gene            complement(3176..3252)
                     /locus_tag="RHV13_004591"
     tRNA            complement(3176..3252)
                     /locus_tag="RHV13_004591"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3216..3218),aa:Asp,seq:gtc)
BASE COUNT          881 a          812 c          807 g          791 t
ORIGIN      
        1 cctttgtccg ctgcattttt gcgagcatca ttccaccgcc agaacaactc aacttcactt
       61 gcacaaataa acaggaataa gccactaaaa ttcacgaatt agcgggctac agcagcgata
      121 tcttatgcac ataatggtga ctcaaggagg gcttatgtca ctgtcaattg ttcatacccg
      181 cgcagccctg ggagtaaatg cgcccccgat cactgttgag gtacatatca gtaaaggtct
      241 acccggctta actatggtgg gcttaccaga aacaactgta aaagaagctc gcgatcgcgt
      301 gcgcagcgcc attatcaata gcggatatga atatccggcg aaaaaaatca ccatcaacct
      361 tgctccagcc gatctaccaa aagaaggggg acgatatgat ttacctatcg ccattgcgtt
      421 gctggcggcc tcagaacagc ttacagccaa taagttagat gaatatgaat tagtcggaga
      481 actggctctt acaggcgctc tgcgtggcgt tcccggcgca atctccagtg caactgaagc
      541 tattaagtcg ggcagaaaaa ttatcgtcgc gaaagataac gaagatgaag tggggctaat
      601 taacggtgaa ggatgcctga tagccgatca tctgcaggct gtctgtgcgt ttctggaagg
      661 taagcacact ctcgaacgcc cgaaaccaac tgatgcagta tcccgggcgc tacaacatga
      721 tctcagtgat gttgtcggtc aggagcaagg aaagcgagga ctggaaatta ccgccgctgg
      781 tgggcacaac cttttactga ttggaccgcc gggaacaggt aaaacaatgc tcgccagccg
      841 tattaatggc ctgttgccag atttaagcaa tgaagaggca ctggagagcg ctgcgatatt
      901 aagtctggta aatgctgaat cagtacaaaa acaatggcgg cagcgcccgt tccgctcacc
      961 tcatcacagt gcgtcgttaa ctgcgatggt gggcggtggt gcaattccag ggccaggtga
     1021 aatttcgctg gcgcataacg gcgtgctttt tcttgatgag ttacctgaat ttgaacggcg
     1081 tacactggat gccttgcgag agccgattga atccgggcag atccatcttt cacgcacacg
     1141 agcaaaaata acctatccag cccgtttcca gcttgttgcg gcgatgaatc ccagccctac
     1201 cggacattat cagggaaacc ataaccgctg cacgccagaa cagacattac gttatctcaa
     1261 ccggctctcg gggccctttc tcgaccgctt cgatctctca ctggagatcc cattaccacc
     1321 ccccggcatt ttgagtaaaa cggtagtgcc gggagaaagc agcaccaccg ttaaacaacg
     1381 tgtaatggct gccagagagc gccaatttaa gcggcagaat aagttgaacg cctggctgga
     1441 tagtccggaa atacgccaat tctgcaaact tgagagcgaa gatgcgatgt ggttggaagg
     1501 aacactgatc catctggggt tatcgattcg tgcctggcag cggttattga aagttgcacg
     1561 aaccattgct gatattgatc agtctgacat tatcacacgt cagcatttgc aggaggcagt
     1621 tagctatcgt gcgattgacc gtttgctcat ccatctgcaa aaactactga cataaaaaaa
     1681 gggcatttcg ccctttttat taatcatcag aatcggtgta gtcttcagca ccttcaacct
     1741 gcggtttacc gccggaaagg gtgtgaaaac gttttggacg cttaatacgc gtcatatact
     1801 tggaccacac gcgttctgct tctgtcactg gctcacgttc gccacggcat actgctacga
     1861 agagtttctc ttcctcggta accggctcgc gtttgccaag atccaactca ttgaaggcat
     1921 aaccatgacg ctcaagcagt tgtgcctctt tgatggtgaa atcaccatga cgagagaatc
     1981 cacgtggata atgtttattg tcgaaatatc gattagtcgt cgtaaagctt tccgccatcc
     2041 tgcacgctcc taattctttg accgagctag ttatggcgcg gagtattagt tacgcttgac
     2101 agagtgtaaa acaaaacatt taaatcataa cgacaaataa ttttgcggag agcactgtgg
     2161 atacggaatt gttaaaaact ttcctggaag ttagccgaac gcgtcacttt ggtcgagcgg
     2221 ctgaatcgct ctatctgacc cagtcagcag tgagctttcg aatcagacaa ctggaaaatc
     2281 aactgggtgt gaaccttttc acccgccaca gaaacaatat ccgtttaacc gctgccggtg
     2341 aaaaactact gccttatgca gaaacgctca tgagcacctg gcaggccgcc cgtaaggagg
     2401 tggcgcatac ctcacgacat aacgagtttt ctatcggtgc cagcgcctca ttgtgggaat
     2461 gtatgcttaa tcagtggctg ggacgtttgt atcaaaatca ggatgcccat acaggcttac
     2521 agttcgaagc gcgaattgcc caacggcagt ctctggtaaa acagctgcat gaacgccagc
     2581 ttgatcttct tattaccact gaagcgccca aaatggacga atttagtagt cagttgctgg
     2641 ggtatttcac tttagcgctt tataccagtg ccccttccaa actaaaggga gatcttaact
     2701 atctgcgact ggagtggggg ccagattttc aacagcatga ggcaggtttg atcggtgctg
     2761 acgaagttcc cattctgaca accagttctg ctgaactggc acagcaacag attgcgatgc
     2821 ttaatggttg cacctggcta cccgtcagct gggcgcgtaa aaaaggcggc ctgcacaccg
     2881 ttgtcgatag cacaacactt tcgcggccgc tttatgccat atggctgcaa aatagcgata
     2941 aaaatacgct gattcgcgat ctattgaaaa ttaacgtgct ggatgaagtg tattaatctg
     3001 aatggctggc aaggatgccg gtagaaggat ttacttcaga gagggttgtt tcagataaaa
     3061 aaaatcctta gctttcgcta aggatgattt ctggcagggg cggagagact cgaactccca
     3121 acacccggtt ttggagaccg gtgctctacc aattgaacta cgcccctaat tagggtggcg
     3181 gaacggacgg gactcgaacc cgcgaccccc tgcgtgacag gcaggtattc taaccgactg
     3241 aactaccgct ccaccgaatt cttttacaac caccggtttt atgaccggct t
//
LOCUS       DAPGOF010000285         3275 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.290, whole genome shotgun sequence.
ACCESSION   DAPGOF010000285 DAPGOF010000000
VERSION     DAPGOF010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.290"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(87..614)
                     /gene="mobB"
                     /locus_tag="RHV13_004592"
     CDS             complement(87..614)
                     /gene="mobB"
                     /locus_tag="RHV13_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HDV1244028.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQNQDG"
     gene            complement(596..1180)
                     /gene="mobA"
                     /locus_tag="RHV13_004593"
     CDS             complement(596..1180)
                     /gene="mobA"
                     /locus_tag="RHV13_004593"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HDV1244029.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPPDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            1250..1519
                     /locus_tag="RHV13_004594"
     CDS             1250..1519
                     /locus_tag="RHV13_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HDV1244030.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1596..2582
                     /gene="srkA"
                     /locus_tag="RHV13_004595"
     CDS             1596..2582
                     /gene="srkA"
                     /locus_tag="RHV13_004595"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HDV1244031.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQTEAVGRYLGRMHQTGHKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTVTFIEQAKVLQEPPLQLTPMY"
     gene            2599..3225
                     /gene="dsbA"
                     /locus_tag="RHV13_004596"
     CDS             2599..3225
                     /gene="dsbA"
                     /locus_tag="RHV13_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HDV1244032.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
BASE COUNT          830 a          792 c          797 g          856 t
ORIGIN      
        1 aggccatcct gacggatggt ctttttgcgt tggtgcaaaa aaatgccgga tgcgacgctg
       61 gcgcgtctgc tccagcctac tcttgcttat ccgtcctgat tttgcatcca ctccaccaca
      121 aaatcagcca gcccctcaac atcattaata tccagtaacg cgacatcaag attaagcggc
      181 acatcactgg ctacagcaat aacatgcctg tctatcacta attcttcagg tcgatgtccg
      241 gctccatcgc gaaacagcac aatctttgcg atctcttcat gcttaaaccc ttcgaccaga
      301 atcaaatcca gctttgaggt atccattcga cttgcgagaa actgtagatc cagctcttct
      361 tcgtctggtg tttccgtcat caaggcccat cgttgctggc tggcaacgat ggtttgtgcc
      421 gcgccagcct tgcgcagctc atagctatct ttgcctggct tatcaacatc catatcatgg
      481 tgcgtatgct taatcagccc tggacggatc cctctggcgc ataatgccgg gatcagtttt
      541 ttcaaaagcg tagtttttcc ggtaccactc cacgcggcaa aggcgagtaa cggtatcatc
      601 gtttttcctg ccatcgggca agctcctctg gcgtattcac gttaacaaat gcatctttat
      661 gatcgctgaa atcaaccgca tgaccgccag ccagacgcat aaatgccatt acccggcgtt
      721 ctcctgcttg cagatattcc agtaataaag gctcaatagc gcggtttacc agagcaatag
      781 tcgggtgatc gcgttcaccg tcatggaccc acacgacagg cgcatctttg cgctgatgat
      841 taagcctggc tgctaaatca gggggaatgt aaggcgtatc gcacgggcaa aacaaaaacc
      901 actcaccagc ttcctgctgc attactgaaa gcattcctgc cagagggcct gggtaatccg
      961 ccagtgaatc ttcaatcact ttcagaccgc ctgcctgata gatttcctga tgacgattag
     1021 cattaaccac gacgtgagag agctgcgtca taagcgcgtc agcgacatgt tgccacagcg
     1081 gtttgccatt taagtccagc aatcctttat ctacgccgcc cattcgtctg gctttaccgc
     1141 ctgccagcac aacgcctgtt atcgtcgtca tcagattcac cgatatcgcc tcttttattg
     1201 tgggattgac cctgctaacg tgtctgtctc aagagtaagg agcatcacta tgaaatgtaa
     1261 acgtctgaat gaagttattg aactcctcca gccagcctgg caaaaagagc cagaccttaa
     1321 cctgctgcaa tttttgcaga aactggcgaa agagtcaggt tttgacggcg aactggcgga
     1381 tttgacggat gacattctga tctatcacct gaaaatgcgt gattccgcga aagatgcggt
     1441 gatcccgggt ttgcagaaag attatgaaga agacttcaaa acggcgctgt tacgcgctcg
     1501 cggcgtaatt aaagagtaaa agcttgtaag cggcgccacc aaaatcatcg tgaaatgata
     1561 tccttcatca ttcgtaatgt tttccggatg atgggatgaa caacagcgct tttactttcc
     1621 agacactaca cccggatacc atcatggacg ctctgtttga gcaagggatc cgggtggatt
     1681 ccggtcttac cccgcttaac agctatgaaa accgtgtcta tcaatttcag gacgaagagc
     1741 gtcgacgttt tgtcgtcaaa ttttatcgcc ctgaacgttg gacagccgat caaatcctcg
     1801 aagaacatca atttgcgttg cagctggtaa atgatgaagt tccggtcgca gcacctgtgg
     1861 cctttaacgg tcagacttta ttgaatcatc agggatttta tttcgctgtt tttccaagcg
     1921 tcggtggtcg ccagttcgaa gctgataata tcgatcagac ggaagcggtt gggcgttatt
     1981 tagggcgtat gcaccagacg gggcacaaac agctttttat ccatcgcccg accatcggtc
     2041 tgaatgaata tctcattgag ccacgcaagc tgtttgagga cgctacactg ataccttccg
     2101 ggttgaaagc ggcattcctg aaagcgacag atgagctgat cgccgccgtt acagcacact
     2161 ggcgggaaga tttcaccgtt ctgcggctac atggagactg ccacgccggg aatattctct
     2221 ggcgcgatgg tccaatgttt gttgatctgg atgatgcacg taatggtcca gctattcagg
     2281 atttgtggat gttgctcaat ggcgataaag ccgagcagcg gatgcaactg gaaactatta
     2341 ttgaagctta tgaagaattt agcgagttcg acaccgctga aatcggactg attgaacctt
     2401 tacgcgccat gcgtttggtt tattatcttg cctggctaat gcggcgttgg gctgatcccg
     2461 cgttcccgaa aaatttcccg tggttaaccg gggaagatta ctggctgcga caaacggtga
     2521 cttttataga acaggcaaaa gttctacaag aacccccttt acaattaaca cctatgtatt
     2581 aatcggagag agtagatcat gaaaaagatt tggctggcgc tggctggttt agttttagcg
     2641 tttagcgcat cggcggcgca gtatgaagat ggtaaacagt acactaccct ggaaaaaccg
     2701 gtagctggcg cgccgcaagt gctggagttt ttctctttct tctgtccgca ctgctatcag
     2761 tttgaagaag ttctgcatat ttctgataat gtgaagaaaa aactgccgga aggcgtgaag
     2821 atgactaaat accacgtcaa cttcatgggt ggtgacctgg gcaaagatct gactcaggca
     2881 tgggctgtgg cgatggcgct gggcgtggaa gacaaagtga ctgttccgct gtttgaaggc
     2941 gtacagaaaa cccagaccat tcgttctgct tctgatatcc gcgatgtatt tatcaacgca
     3001 ggtattaaag gtgaagagta cgacgcggcg tggaacagct tcgtggtgaa atctctggtc
     3061 gctcagcagg aaaaagctgc agctgacgtg caattgcgcg gtgttccggc gatgtttgtt
     3121 aacggtaaat atcagctgaa tccgcagggt atggatacca gcaatatgga tgtttttgtt
     3181 cagcagtatg ctgatacagt gaaatatctg tccgagaaaa aataattcaa tgtaaattac
     3241 ataaagcccg tgaatattca cgggcttttt ttatt
//
LOCUS       DAPGOF010000286         3223 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.291, whole genome shotgun sequence.
ACCESSION   DAPGOF010000286 DAPGOF010000000
VERSION     DAPGOF010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.291"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..2064
                     /gene="rhsB"
                     /locus_tag="RHV13_004597"
     CDS             <1..2064
                     /gene="rhsB"
                     /locus_tag="RHV13_004597"
                     /inference="COORDINATES: protein motif:HMM:NF017411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HDV1244033.1"
                     /translation="RGELAAVYDRSNTQVRSFTYDDKYRGRMVAHRHTGRPEIRYRYD
                     RDGRVTEQLNPAGLSYTYQYEKDRITITDSLNRREVLHTQGEAGLKRVVKKEHADGSV
                     TQSQFDAVGRLKAQTDAAGRTTEYSPDVVTGLITRITTPDGRASAFYYNHHSQLTSAT
                     GPDGLEMRRKYDESGRLIQETAPDGDITRYRYDNPHSDLPCATEDATGSRKTMTWSRY
                     GQLLSFTDCSGYVTRYDHDRFGQVTAVHREEGLSQYHAYDSRGQLTAVKDTQGHETRY
                     EYNIAGDLTAVIAPDGSRNGTQYDAWGKAVRTTQGGLTRSMEYDAAGRVIRLTSENGS
                     HTTFRYDVLDRLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTHWHYDEADRLTQRTV
                     KGETAEQWQYDERGWLTDISHISEGHRVTVHYGYDEKGRLTGERQTVHHPQTEALLWQ
                     HETRHAYNAQGLANRCIPDSLPAVEWLTYGSGYLAGMKLGDTPLVDFTRDRLHRETLR
                     SFGRYELTTAYTPAGQLQRQHLNSLQYDRDYTWNDNGELIRISSPRQTRSYSYSDSGR
                     LTGVHTTAANLDIRIPYATDPAGNRLPDPELHPDSTLSMWPDNRIARDAHYLYRYDRH
                     GRLTEKTDLIPEGVIRTDDERTHRYHYDSQHRLVHYTRTQYEEPLVESRYLYDPLGGK
                     TGVAA"
     gene            2444..>3223
                     /locus_tag="RHV13_004598"
     CDS             2444..>3223
                     /locus_tag="RHV13_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDV1244034.1"
                     /translation="MYTPARKIHLYHCDHRGLPLALISAEGATEWYAEYDEWGNLLNE
                     ENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNFYQYPLN
                     PISNIDPLGLETLKCIKPLHSMGGTGERSGPDIWGNPFYHQYLCVPDGKGDYTCGGQD
                     QRGESKGDGLWGPGKASNDTKEAAGRCDLVETDNSCVENCLKGKFKEVRPRYSVLPDI
                     FTPINLGLFKNCQDWSNDSLETCKMKCSGNNIGRFIRFVFTG"
BASE COUNT          804 a          901 c          960 g          558 t
ORIGIN      
        1 cgcggcgaac tggcggcggt gtatgaccgc agcaatacac aggtgcgcag ctttacttac
       61 gatgataaat accggggccg gatggtggcg caccgtcaca cgggccggcc ggaaatccgt
      121 taccgttacg accgcgacgg gcgggtgaca gaacagctaa acccggcagg cttaagctac
      181 acctatcagt atgagaagga ccgcatcacc atcaccgaca gcctgaaccg ccgtgaagtg
      241 ctgcacacgc agggtgaagc cgggctgaag cgggtggtga aaaaggaaca cgcggacggc
      301 agcgtcacgc agagtcagtt tgacgccgtg ggcaggctta aggcacagac ggatgccgca
      361 ggccggacaa cagaatacag cccggatgtg gtgacgggcc tcatcacgcg catcaccacg
      421 ccggatggca gggcatcggc gttttactat aaccaccaca gccagttaac gtcagccacc
      481 gggcctgacg ggctggaaat gcgccgcaaa tatgatgaat ccggccgtct gattcaggaa
      541 actgcccctg acggcgatat cacccgctac cgttatgata atccacacag tgacttaccc
      601 tgcgcaacgg aagatgccac cggcagccgg aaaaccatga cgtggagccg ttacggtcag
      661 ttgctgagct tcactgactg ttccggttat gtaacccgtt atgaccatga ccgttttggt
      721 caggtgacgg cggtgcaccg cgaggaaggg ctgagccagt accacgcata cgacagccgt
      781 ggacagttaa ctgcggtgaa agacacgcag ggccatgaaa cgcggtatga atacaacatc
      841 gccggtgacc tgaccgccgt cattgccccg gacggcagca gaaacgggac acagtacgat
      901 gcgtggggaa aggccgtccg taccacgcag ggcgggctga cccgcagtat ggaatacgat
      961 gctgccggac gggtcatccg cctgaccagt gaaaacggca gccacaccac cttccgttac
     1021 gatgtactcg accggctgat acaggaaacc ggctttgacg gccgcacaca gcgttatcac
     1081 cacgacctga ccggcaaact tatccgcagc gaggatgagg ggctggtcac ccactggcac
     1141 tatgacgaag cagaccgcct cacgcagcgc accgtgaagg gtgaaaccgc agagcagtgg
     1201 cagtatgacg aacgcggctg gctgacagac atcagccata tcagcgaagg gcaccgggtg
     1261 acggtgcatt acgggtatga tgagaaaggc cggctgaccg gtgagcgtca gacggtgcat
     1321 cacccgcaga cggaagcact gctctggcag catgagacca gacacgctta caacgcgcag
     1381 gggctggcga accgctgtat accggacagc ctgcccgccg tggaatggct gacctacggc
     1441 agcggttacc tggcaggcat gaagctcggc gacacaccgc tggtggattt cacccgcgac
     1501 cgcctgcacc gggaaacgct gcgcagcttc ggccgttatg aactcaccac cgcttacacc
     1561 cctgccgggc agttacagcg tcagcacctg aacagcctgc agtatgaccg cgattacacc
     1621 tggaacgaca acggcgaact catccgcatc agcagcccgc gccagacccg gagttacagc
     1681 tacagcgact ccggcaggct gaccggcgtc cacaccaccg cagcgaacct ggatatccgc
     1741 atcccgtatg ccacggaccc ggcgggcaac cgcctgcccg acccggagct gcacccggac
     1801 agcaccctca gcatgtggcc ggataaccgt atcgcccgtg acgcgcacta tctttaccgg
     1861 tatgaccgtc acggcaggct gacagagaaa accgacctca tcccggaagg ggttatccgc
     1921 acggatgatg agcggactca ccggtaccat tacgacagtc agcaccggct ggtgcactac
     1981 acgcggacac aatatgaaga gccgctggtc gaaagtcgct atctttacga cccgctgggt
     2041 ggcaaaacgg gtgtggcggc gtgaacggga cctgacgggc tggatgtcgc tgtcacggaa
     2101 accgcaagtg acctggtacg gctgggacgg cgaccggctg accacaatac agaacgacag
     2161 aacccgcatc cagacgattt atcagccggg gagcttcacg ccactcatca gggttgaaac
     2221 cgccaccggt gagctggcga aaacgcagcg ccgcagcctg gcggatgcgc ttcagcagtc
     2281 cggcggcgaa gacggtggca gtgtggtgtt cccgccggtg ctggtgcaga tgctcgaccg
     2341 gctggaaagt gaaatcctgg ctgaccgggt gagtgaggaa agccgccgct ggctggcatc
     2401 gtgcggcctg acggtggagc agatgcaaaa ccagatggac ccggtgtaca cgccggcgcg
     2461 aaaaatccac ctgtaccact gcgaccatcg cggcctgccg ctggcgctta tcagtgcgga
     2521 aggggctaca gaatggtacg cagaatacga tgaatggggc aacctgctga atgaagagaa
     2581 cccgcatcag ctgcagcagc ttatccgcct gccgggtcag cagtatgatg aggagtccgg
     2641 cctgtattac aaccgccacc gctattatga cccgctgcag gggcgatata tcactcagga
     2701 tccgattggg ctgaaagggg gatggaattt ttatcagtat ccgttgaatc cgatctcaaa
     2761 tatagatcca ttaggattag aaacactaaa atgcattaag ccactgcatt caatgggcgg
     2821 aactggtgaa agaagcggtc cagatatatg ggggaatccg ttctatcatc aatatctttg
     2881 tgtcccagat ggtaaagggg actatacttg tggtggccaa gaccaacggg gagaatcaaa
     2941 aggagatggt ctatgggggc caggtaaagc aagtaatgat acaaaagaag ctgctggccg
     3001 ttgtgacctc gttgaaaccg ataatagttg tgtggagaac tgtttaaaag ggaagtttaa
     3061 agaggtaagg ccgcgttatt ctgtattgcc tgatatattc acacctataa atttagggct
     3121 atttaaaaac tgccaagact ggtctaatga ttctttagaa acatgtaaga tgaagtgctc
     3181 cggaaataac attggacgtt ttattagatt tgtattcacc gga
//
LOCUS       DAPGOF010000287         3213 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.292, whole genome shotgun sequence.
ACCESSION   DAPGOF010000287 DAPGOF010000000
VERSION     DAPGOF010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.292"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            146..1039
                     /locus_tag="RHV13_004599"
     CDS             146..1039
                     /locus_tag="RHV13_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV1244035.1"
                     /translation="MLKENFNELQIFLVVARERSFTKAAGKLGVSQSALSHAMKALEE
                     RLNIRLLTRTTRSVAPTEAGERIIACLEPRIDELEQELESLIQQNGTPSGNIRLSAGE
                     HAARSLVWPKLKPFLRKYPEISVELVVDNGFVDIVEGRFDAGIRLGENVDKDMVAVRI
                     GPDMRMAVVGAPAYFAANPAPETPHELQNHRCINMRLPTAGGLYHWEFEREGKTLRVK
                     VDGQLTCSPLPERIDAALSGFGIACVPEDMVQEYIKSGKLIQVLQELCPTFPGYYLYY
                     PSRKQHPPAFALLIDALRYTE"
     gene            complement(1071..2060)
                     /locus_tag="RHV13_004600"
     CDS             complement(1071..2060)
                     /locus_tag="RHV13_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDV1244036.1"
                     /translation="MQKRYLGKSGLEVSALGLGCMGLSHGYGPVTDTRQAIELIRAAV
                     ERGVTFFDTAEVYGPYLNEEVVGEALKPFRDRVVIATKFGFTFGDDNKQQILNSRPEH
                     IREAVVGSLRRLKTDVIDLLYQHRVDPDVPIEDVAGTVKDLIAEGKVKHFGLSEAGAQ
                     TIRRAHAVQPVTALQSEYSMWWREPEQEILPLLEELGIGFVPFSPLGKGFLTGSIKPG
                     TTFGKDDYRSTVPRFAAQAIEANEKLVTLLGELAAEKGVTSAQIALAWLLAQKPWIVP
                     IPGTTKLHRLEENLAAADIVLSQKDTQQITEALETIKIVGERYSPEHQARVGR"
     gene            complement(2087..2938)
                     /locus_tag="RHV13_004601"
     CDS             complement(2087..2938)
                     /locus_tag="RHV13_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDV1244037.1"
                     /translation="MQKVKLNNGIEMPLLGFGVFQMTDAAECERAVIDAINTSYRLID
                     TAASYQNETQVGNALKQSGIARNELFVTTKLWLQDTSYEGAKAQFERSLNRLQLDYVD
                     LYLIHQPYGDVHGAWRAMEELQQAGKIRAIGVSNFHPDRLADLIAFNNVVPAVNQVEV
                     NPFNQQLHAVPWMQSRGIQPEAWAPFAEGKNGLFQHPVLTAIGEKYGKSVGQVVLRWI
                     YQRGIVSLAKSVRKERMEENINILDFELSPEDMLQITAMDTATSAFFSHRDPAMVEWL
                     TGRKLDV"
BASE COUNT          791 a          770 c          807 g          845 t
ORIGIN      
        1 ttcataataa atctcctgaa agtttagaat tattttcgat acagccagat tatcattgtt
       61 actaataatg tggctaaaat aatagcatct atgccccccc ttcatgagtg tctgtgaaga
      121 gaatgtttct acaaggaaat cgccaatgct caaagaaaac ttcaacgaac tgcaaatctt
      181 tcttgtggtg gcaagagagc gaagttttac caaagcagcg ggcaaactcg gcgtctctca
      241 gtctgcactt agccatgcaa tgaaggctct ggaggaaagg ctcaatatcc gcctcctgac
      301 acggactacc cgcagtgtgg ctcccacaga agcgggggag agaatcattg cctgccttga
      361 accgcgtatt gacgagcttg aacaggaact ggagtcactt attcaacaga acggcacgcc
      421 ctccgggaat attcgtttat ctgccgggga acatgccgcg cgaagtctgg tctggccgaa
      481 gctaaaacct tttctcagaa agtatcccga aatcagtgtc gaactggtgg tggataacgg
      541 ttttgtcgat attgttgaag gccgttttga tgccgggata cgactgggtg aaaatgttga
      601 taaagatatg gttgcggtca gaattggacc agatatgcgg atggctgtgg tgggggcacc
      661 ggcgtatttt gctgcaaacc ctgcgcctga aacgcctcac gagctacaaa accatcgctg
      721 catcaacatg cgtttgccaa ctgccggtgg actttaccac tgggagttcg agcgagaagg
      781 gaaaactctt cgcgttaagg ttgatgggca gctgacgtgt agtccattgc cagaaagaat
      841 tgatgccgcg ttatcagggt ttgggatcgc ttgtgtccct gaagacatgg ttcaggagta
      901 tataaaatca ggcaagctta ttcaggtgtt gcaggagttg tgtccaactt ttcccggata
      961 ttatctctac taccccagcc gcaagcagca tccgccagct tttgcgctgt tgatcgatgc
     1021 gcttcgctat accgaataac gggtcctttg ttcctggacc cgtttgggca ttagcggcct
     1081 acgcgtgcct ggtgttcagg cgagtaacgt tcaccgacga ttttaattgt ttccagcgcc
     1141 tcggttatct gctgcgtatc tttctgcgaa agaacaatgt cagcagcggc caggttttct
     1201 tccagccggt gcagtttagt ggtgccaggg atgggaacaa tccacggctt ttgtgctaac
     1261 agccatgcca gtgcgatttg tgcagacgtt acccctttct ccgcagccag ttcacctaac
     1321 aaggtgacca gtttttcatt agcttcaatg gcttgtgctg cgaaacgcgg tacggtgctg
     1381 cggtaatcat ctttgccgaa agtggttccc ggcttaatcg acccggtcag gaaaccttta
     1441 cctagtgggc tgaaggggac aaaaccaatc cccagttcct ccagcaacgg cagaatttcc
     1501 tgttcaggct cacgccacca catggaatat tcgctttgca gggcggtgac aggttgtacc
     1561 gcatgcgcac gacgaatggt ttgtgctccc gcttcagaca ggccgaaatg tttgacctta
     1621 ccttctgcga tgaggtcttt caccgttcct gcaacatctt caataggaac atccggatca
     1681 acacggtgtt ggtacagcag gtcaataaca tctgtcttca gacgacgtaa tgatcctaca
     1741 acggcttcac ggatatgttc cggacggctg tttaaaatct gctgcttatt atcatcgccg
     1801 aaggtaaaac cgaatttagt ggcaattacc acgcggtcac gaaacggttt taaggcttcg
     1861 ccgaccactt cttcattcag atacggacca tacacttcgg cggtatcgaa gaaggtgacg
     1921 ccacgttcaa ccgcagcacg aataagttcg atagcctgac gggtatcggt caccgggccg
     1981 tagccgtggc ttaagcccat gcaaccgagc ccaagggcgg agacttcgag tccggattta
     2041 cccagataac gtttttgcat tgaattaata cctcttttat cgcggcttaa acatcaagtt
     2101 tgcggccagt cagccattcc accattgcgg gatcgcgatg agagaagaac gcgctggttg
     2161 cggtatccat ggcggtaatt tgcagcatat cttcaggact gagttcaaaa tcgagaatgt
     2221 tgatgttctc ttccatgcgt tctttgcgta ctgattttgc aagcgaaaca atgcctcgtt
     2281 ggtagatcca gcgcagaaca acctggccta cgcttttgcc atacttctcg ccaattgccg
     2341 tgagaacagg atgctggaac agaccatttt tcccttccgc aaacggtgcc caggcttctg
     2401 gctgaatgcc acgactttgc atccacggaa cggcgtgtag ctgctgattg aaagggttaa
     2461 cttcaacctg gtttaccgct ggaacgacgt tattaaaggc gataaggtca gccagtcggt
     2521 caggatggaa gttactgacg ccaatagcgc gaattttacc ggcctgttgc agctcttcca
     2581 tggcgcgcca ggcaccgtgg acatcaccgt aaggctgatg aatcaaatac aggtcaacgt
     2641 aatcaagttg cagtcgattc agagagcgtt cgaactgggc tttggcccct tcgtaactgg
     2701 tatcctgcaa ccagagtttc gtggtgacaa agagttcgtt acgggcgata ccgctttgtt
     2761 tcagtgcgtt cccgacctgg gtttcatttt gataggaggc ggcggtgtca atcagtcgat
     2821 aactggtgtt aatagcgtca atgaccgctc tttcgcattc agcggcatcg gtcatctgaa
     2881 atacaccaaa gcccaacagg ggcatttcaa taccgttgtt cagttttact ttttgcatgg
     2941 tgttatcctt ccgttctcgt tcatgagaag cataacgtaa agggaaaagc tcgattagac
     3001 ggcaggattt gtcaggggtt atgaacgaaa ttcataaatc tgtttgagtg ttgcgatggg
     3061 tagtgcaagt tcgatatctc cgcaatttac agtccgatga aggaaaatga atatccataa
     3121 aaaatatatc ggtttatcct ggcgtatata cctacttcga cgtatttcca atagttgcta
     3181 attaaaggca ggtcattatt attcactctg aat
//
LOCUS       DAPGOF010000288         3062 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.293, whole genome shotgun sequence.
ACCESSION   DAPGOF010000288 DAPGOF010000000
VERSION     DAPGOF010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.293"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(239..315)
                     /locus_tag="RHV13_004602"
     tRNA            complement(239..315)
                     /locus_tag="RHV13_004602"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(279..281),aa:Asp,seq:gtc)
     gene            complement(448..1179)
                     /gene="dnaQ"
                     /locus_tag="RHV13_004603"
     CDS             complement(448..1179)
                     /gene="dnaQ"
                     /locus_tag="RHV13_004603"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HDV1244038.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRLVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            1244..1711
                     /gene="rnhA"
                     /locus_tag="RHV13_004604"
     CDS             1244..1711
                     /gene="rnhA"
                     /locus_tag="RHV13_004604"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HDV1244039.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1708..2430)
                     /locus_tag="RHV13_004605"
     CDS             complement(1708..2430)
                     /locus_tag="RHV13_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDV1244040.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKP
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2464..>3062
                     /gene="gloB"
                     /locus_tag="RHV13_004606"
     CDS             2464..>3062
                     /gene="gloB"
                     /locus_tag="RHV13_004606"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HDV1244041.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTSSQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKN"
BASE COUNT          840 a          730 c          711 g          781 t
ORIGIN      
        1 acaaaatcaa tttgaaatat acattcattt aatcaatata aatttattct ctggaaatat
       61 atttaagctg aatggttcga tatcattatt gaattttaat gaatgagaca acaagcgaga
      121 atcacgcact tacgccagag taaaatcatt gaacagagaa atggtggaat tcaaatacaa
      181 aaaaaccgca aaattaaaaa tcttgcggtt ctctgaactc attttcatga gtgaatagtg
      241 gcggaacgga cgggactcga acccgcgacc ccctgcgtga caggcaggta ttctaaccga
      301 ctgaactacc gctccgcgtt gtgttccgtt gggaacgagg cgaatattac ggattgcctc
      361 acccttcgtc aacggttttt ctcatctttt gaatcatttg ctgcaaaaat cgcccaagtc
      421 gctattttta gcgcctttta catgtattta tgcccgccag aggcagcttc cgcccttttt
      481 ctgcaccaga tcgagacggg cttcatgtgc tgcaagctct tcatctgttg caaaaacaac
      541 gcgtaactta cttgcctgac gtacaaggcg ctggattgtt gcttcacctt gttgctgttg
      601 tgtctctcct tccatcgcaa aagccatcga cgtttgacca ccggtcatcg ccagataaac
      661 ttccgcaagg atctgggcat cgagtaatgc cccgtgcagc gttcgtttac tgttatctat
      721 ttcgtagcga gcacataacg catcgaggct gttgcgctta ccgggaaaca ttttcctcgc
      781 caccgcaagg ctatcggtga ccttacagaa ggtattggtc ttcggaatat cgcgcttaag
      841 caacgaaaac tcgtagtcca taaagccgat atcgaacgct gcgttatgga tcaccaactc
      901 cgcgccgcgg atatagtcca tgaactcatc ggctacttcg gcaaacgtgg gcttatcgag
      961 caaaaattca tcggcaatac catgtacgcc aaaggcttcc ggatccacca gccgatcggg
     1021 tttgagataa acatggaagt tattgcccgt caggcgacgg ttcaccactt caacggcacc
     1081 aatctcaatg atcttgtggc cttcatagtg cgcaccaatc tggttcatac cggtggtttc
     1141 ggtatcgaga acgatctggc gtgtaattgc agtgctcata gcggtcattt atgtcagact
     1201 tgtcgtttta cagttcgatt caattacagg aagtctacca gagatgctta aacaggtaga
     1261 aattttcacc gatggttcgt gtctgggtaa tcctggacct gggggttacg gcgctatttt
     1321 acgctatcgc ggacgcgaga aaacctttag cgcaggctac acccgcacca ccaacaaccg
     1381 tatggagttg atggccgcta ttgtcgcgct ggaggcgtta aaagaacatt gcgaagtcat
     1441 tttgagtacc gacagccagt atgtccgcca gggtatcacc cagtggatcc ataactggaa
     1501 aaaacgtggc tggaaaacag cagacaaaaa accagtaaaa aatgtcgatc tctggcaacg
     1561 tcttgatgct gcattggggc agcatcaaat caaatgggaa tgggttaaag gccatgccgg
     1621 acacccggaa aacgaacgct gtgatgaact ggctcgtgcc gcggcgatga atcccacact
     1681 ggaagatacg ggctaccaag tcgaagttta agcctgtggt ttacgacatt gccgggtggc
     1741 tccaaccgcc tggcgaattc gtggcttgtt tttactcggt ttcatcgggt ttagcgttaa
     1801 aggaatagtc cgtttccggg caacaataag ttgtaagcaa ccaagcgcag gaatatgcgc
     1861 attcaatagt tttcctccgt gtttgttcca cgggagaaca tggaaacggc tggcgtgcag
     1921 cacttcaaaa ttcaacaaag agagccagtc cagttgccgc atcagagtaa acatccggct
     1981 gttatagggc gaggtttttc gcaataccgg cacaagtttg cgtaatccca ttaaactaat
     2041 gggattgaag ccactaatca ccagccagcc atcatcaatc aatacccgat cggcttcacg
     2101 caataaacga tgcggatcgg tgcaccacgg caatgtatgt gccaatagac aaacatcaac
     2161 ggatttatcg gcaaaaggaa gatgaagtgg gtccgcctgc acctggacag gcattccttg
     2221 cgcagaaaca ttcacttgat gagaaaccgc gcacgcttcg caattgattt ctgcgcttaa
     2281 attgccaatc ttaagcagat gaaaaccata cattttagtg aaccacgggt tgagctggcg
     2341 ctccagcgcc ttgcgataaa gctctcccca gggcaaatcg ccccagcaat caggagccac
     2401 gacagtttga gggactcttg ccggtttcat cacaaccttc cgtttcacac tgagaggtaa
     2461 tctatgaatc ttaacagtat tcccgccttt gatgacaatt acatctgggt tttgaatgat
     2521 gaagcaggtc gctgcctgat tgtcgatccc ggagacgcag agccagtatt aaacgccatt
     2581 gccgccaata actggcaacc ggaggccata tttctcaccc accatcacca cgatcacgtt
     2641 ggcggcgtaa aagaactggt ggaaaagttt ccgcaaattg tggtgtatgg cccacaagag
     2701 acacaagata agggaacaac acaggtagtc aaagatggcg aaaccgcctt cgttttgggg
     2761 catgaattta gtgtaattgc cacaccgggt cacactttag gacatatctg ttacttcagt
     2821 aaaccttatc tattttgcgg cgacacgctg ttttctggtg ggtgtggtcg gttgtttgaa
     2881 gggacatcat cacaaatgta tcaatcactt aaaaagttaa gtgcgttacc tgacgataca
     2941 ttggtatgtt gtgctcatga atatacctta tcaaatatga agtttgcttt gagtattctt
     3001 ccgcacgatt tgtccataaa tgattattat cgtaaagtta aggagttacg ggcaaaaaat
     3061 ca
//
LOCUS       DAPGOF010000289         3045 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.295, whole genome shotgun sequence.
ACCESSION   DAPGOF010000289 DAPGOF010000000
VERSION     DAPGOF010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.295"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1277
                     /gene="sctN"
                     /locus_tag="RHV13_004607"
     CDS             <1..1277
                     /gene="sctN"
                     /locus_tag="RHV13_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system ATPase SctN"
                     /protein_id="HDV1244042.1"
                     /translation="LYKYSYLHSINGSLIEAELDDVSVGEVCEIYASWQANERIARAQ
                     VVGFRNGKTLLNLIGSSVGLTRTAVLKPTGEQLTIQISDAFLGSVLNASGQIMERFVP
                     DTPGDRGNLRLIDELPPSYQERRVINTPLETRIRVIDGVLTCGIGQRVGIFASAGCGK
                     TVLMHMLVNNTEADVFVIGLIGERGREVTECAESLKQSVNAAKCVLVYATSDFSSVDR
                     CNAALMATTVAEYFRDRGKRVVLFIDSMTRYARALRDMKLAAGEPPARRGYPASVFDS
                     LPRLLERPGPTLKGSITAFYTVLLEGEDESDPLGDEIRSILDGHIYLSRKLAGQGHYP
                     AIDVLKSVSRVFGQVTDEKHRDNAARVRKILTTLEDLQVFIDLGEYRAGQNAENDFAM
                     NARSKLTNWLKQSVNEKMPMSETLKELERIVK"
     gene            1267..1725
                     /locus_tag="RHV13_004608"
     CDS             1267..1725
                     /locus_tag="RHV13_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HDV1244043.1"
                     /translation="MLSKVNRLIRRTAQSLAACEASLQKLNAEKEKLAEKERLYDMQL
                     KNLQSLLDMKELLGEVVFRQDIFYSLRKVAVIQQQIAEINLEKQKIAERRKILNKEIV
                     QQQAQRKHWWLKGEKYERLKTRIKKKLLNQMLYQDELEQEEKYNGRSQEN"
     gene            1703..2578
                     /locus_tag="RHV13_004609"
     CDS             1703..2578
                     /locus_tag="RHV13_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HDV1244044.1"
                     /translation="MDEVKKIEHGKILNTDTPDNSASDLDSFLKKNKKNKEMSEIVAG
                     TILQLLRQTQDSQFSTLGKKNGQMKSIDNKSSLQVDRNPSLSLQTNGTEQFSTKKILN
                     MTSRGQGISSELSDNTIKIKEKTHNGSHTEYITDQKNSNNKDREKKYRDGDKINGPQA
                     HSLDSTNERRFADNRTMFTQHIEKQRNVHTLNQNDINNSVNSVNVPENELTYQFQRWG
                     QNHTVRILESSEGIRLKPSDALVSDRLHEAQNNDVTAQRWVLTEQDERQGQRHQPHEE
                     QENEGKFENDQKDES"
     gene            2578..>3045
                     /locus_tag="RHV13_004610"
     CDS             2578..>3045
                     /locus_tag="RHV13_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
                     /protein_id="HDV1244045.1"
                     /translation="MFGLRKVNRNTHTFETTFQNWKENGEDVALLMPEFSAKWLPIAE
                     ESGSWSGWVLLREIFPLISSELAGMALMPETERLIGEWLSLSSSPLNLKHPELKYNRL
                     CVGKVFDGVLSPAQPLIRIWTGELNLWLDKVTVCQYGNAPALDKKSLYWSIHFV"
BASE COUNT         1046 a          547 c          692 g          760 t
ORIGIN      
        1 tattatataa atacagctac ttacatagca taaacggctc tctgattgaa gcagaacttg
       61 acgacgtttc tgtgggtgaa gtatgcgaaa tttatgcgag ctggcaagca aatgaacgta
      121 tagccagggc tcaggtcgtt ggctttcgca atggaaagac attattaaac cttataggca
      181 gctcagtggg gcttacacgt actgcagttt tgaaaccaac gggagagcag ttaaccatac
      241 agatcagtga tgcctttctt ggttcggttt tgaatgcctc tggtcaaatt atggaaaggt
      301 ttgtcccgga cactccaggc gatagaggaa atttacgcct gattgatgaa ctgccaccgt
      361 cgtatcagga acgtcgagtt attaatacac cactagagac acgcataaga gtcattgatg
      421 gcgtactgac atgtggcatt ggtcaacgtg ttgggatttt cgcatcggct ggctgtggca
      481 aaacggtttt aatgcatatg ctcgtaaaca atactgaagc tgatgtgttt gttattggct
      541 tgattggcga acgtggtagg gaagttacgg aatgcgcgga atcgctgaaa caatcagtaa
      601 atgcagcgaa atgcgtattg gtatatgcca cctctgattt ttcgtcagta gaccggtgta
      661 atgccgccct gatggcgaca accgtggcag agtattttag agatcgagga aagagagttg
      721 tactttttat cgactcaatg acgcggtatg cacgagcact acgtgatatg aaactggcag
      781 cgggtgaacc acctgccaga cgtggttatc ctgcttctgt ttttgacagt ttgcctcgct
      841 tactggagag gccgggacca acactaaaag gaagtatcac cgcgttttat accgtgttac
      901 tggaagggga agacgaatca gaccccctgg gtgatgaaat tcgctcaatc cttgatggcc
      961 atatctatct aagtcgcaaa ctcgctgggc aaggtcatta tccggcaata gatgttctaa
     1021 aaagtgtgag tcgagtattt ggtcaggtca ctgatgaaaa acacagagat aatgctgcac
     1081 gggtaaggaa gatcctcaca actttggaag atctacaggt gtttattgac ttaggggaat
     1141 atcgcgctgg gcaaaatgct gaaaatgatt ttgctatgaa tgcccggtct aaactcacaa
     1201 attggttgaa acagtcagtt aatgaaaaaa tgccaatgag tgaaacgcta aaagagcttg
     1261 aacgaattgt taagtaaagt taatcgactt ataagaagaa cagcgcagag cctggctgca
     1321 tgtgaagcat cattacaaaa gttaaatgca gaaaaagaaa agttagccga aaaggaacgc
     1381 ctgtatgata tgcaacttaa aaacctgcag tcactgttag atatgaaaga gttactcggc
     1441 gaagttgtat ttcgacagga tatattttac tcgttacgta aagttgcagt cattcagcag
     1501 cagatcgctg agataaatct tgaaaagcaa aaaatagcag agcggagaaa aatacttaac
     1561 aaggaaatag ttcaacagca agcacaaaga aaacactggt ggctaaaagg agaaaaatac
     1621 gaacgactaa aaacgcgcat caaaaaaaaa cttttaaatc agatgcttta tcaagacgag
     1681 cttgaacagg aggaaaaata taatggacga agtcaagaaa attgaacacg gtaaaatttt
     1741 aaacacagac acacccgata attctgcttc ggatttagac agtttcttaa agaaaaacaa
     1801 aaaaaataaa gaaatgagtg agattgtcgc tggaaccatt ctccagttat tacgtcagac
     1861 tcaggatagc caattttcta cattggggaa aaagaatggg caaatgaagt ctattgacaa
     1921 caaatcatct ctccaggttg acaggaatcc atctctttct ttgcaaacaa atggaacaga
     1981 gcaattttct acaaagaaaa ttctcaatat gacttctcga ggtcagggta ttagttctga
     2041 actatctgat aacacaataa aaattaagga aaaaacccat aatggtagcc atacagagta
     2101 catcacagat caaaaaaaca gtaataataa agatcgtgaa aaaaaatacc gcgatggaga
     2161 caaaataaat ggaccgcagg ctcattcatt agacagtact aacgaaagaa ggtttgctga
     2221 taacagaacc atgtttactc aacatatcga aaagcaaagg aatgttcata cgctcaatca
     2281 gaatgatata aataattccg ttaatagtgt caatgtgcca gaaaatgaat tgacatatca
     2341 gtttcagcgt tggggacaga atcatacagt ccggattctg gaaagtagtg aagggatacg
     2401 actaaaacca tcagacgcgc ttgtctccga cagactccat gaagcccaaa acaatgatgt
     2461 tacagcacaa cgttgggtat tgactgagca agatgaacga caaggacaac gacatcagcc
     2521 tcatgaggag caagaaaatg aaggcaaatt cgaaaacgat cagaaggatg aaagttaatg
     2581 ttcggtctga ggaaagtaaa tcgaaacact catacgtttg aaaccacttt tcaaaattgg
     2641 aaagaaaatg gcgaagatgt tgcattatta atgcccgaat ttagtgctaa atggctacca
     2701 atagcagaag agagtggcag ctggtctgga tgggtattgc ttcgagaaat atttccctta
     2761 atttcttctg agctcgctgg gatggcttta atgccagaaa cggaaaggtt aataggggaa
     2821 tggctcagtt tatccagttc cccgttaaat ctaaagcatc ctgaactaaa atataatcgt
     2881 ttgtgcgtag gtaaagtatt tgatggagta ttgagcccag ctcaaccatt aataagaata
     2941 tggacagggg aattgaatct ttggttagat aaagtcacag tctgccaata cggaaacgct
     3001 ccagcgttag acaaaaaatc attatattgg tccattcatt ttgta
//
LOCUS       DAPGOF010000290         3037 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.296, whole genome shotgun sequence.
ACCESSION   DAPGOF010000290 DAPGOF010000000
VERSION     DAPGOF010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.296"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1..382
                     /locus_tag="RHV13_004611"
                     /pseudo
     CDS             1..382
                     /locus_tag="RHV13_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid-binding protein"
     gene            390..1076
                     /locus_tag="RHV13_004612"
     CDS             390..1076
                     /locus_tag="RHV13_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDV1244046.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1054..1677)
                     /gene="tetR(B)"
                     /locus_tag="RHV13_004613"
     CDS             complement(1054..1677)
                     /gene="tetR(B)"
                     /locus_tag="RHV13_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HDV1244047.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1759..2964
                     /gene="tet(B)"
                     /locus_tag="RHV13_004614"
     CDS             1759..2964
                     /gene="tet(B)"
                     /locus_tag="RHV13_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HDV1244048.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
BASE COUNT          805 a          572 c          687 g          973 t
ORIGIN      
        1 ttgatgttca cgttgtttta gataatcaaa taggacaatt agcattacta ggaaaaacat
       61 taggtaataa aggtatgggt tggaaggggg agggatattt acggttggtg atgaatgcca
      121 tgctcatttt cttgttgaac aaggaaagga agctaaaata gcgctagagc aagctggact
      181 gttagtactt gcgatccgga caccattaat tcgtaagtta aaacaggaaa aaccggggga
      241 acttggcgaa atagcacgag tattggcgga gaataacatt aatattttag tgcaatacag
      301 tgaccatgct aaccaactga tattaataac ggacaatgat agtatggctg catctgttac
      361 gctcccttgg gcaataaagt gaacttgcga tggctaattt aatacgaaaa gaggttacct
      421 ttgagtcctc aatagccgcg ataggggcgg ctatgtctga catttcacga gttaaaatac
      481 tcagtgcttt gatggatggg cgagcttgga cggccactga gctaagttct gtggcgaata
      541 tatcagcttc aacggcgagc agtcatttat ctaaattatt agattgccag ctaatcacag
      601 tagtagctca aggcaagcat cgttattttc ggctagcagg aaaagatatt gctgaattga
      661 tggaaagtat gatggggatc tccttaaacc atggcgtaca tgccaaagtt tccacgccag
      721 tgcatttacg aaaagcacgt acttgctatg atcatttagc tggcgaagtt gccgttaaga
      781 tctatgattc cctttgtcaa cagcaatgga tcactgaaaa tggttcaatg atcacattaa
      841 gtggtattca atattttcat gaaatgggaa ttgacgttcc ttccaaacat tcacgtaaaa
      901 tctgttgtgc gtgtttagat tggagtgaac gccgtttcca tttaggtggg tacgttggag
      961 ccgcattatt ttcgctttat gaatctaaag ggtggttaac tcgacatctt ggttaccgtg
     1021 aagttaccat cacggaaaaa ggttatgctg cttttaagac ccactttcac atttaagttg
     1081 tttttctaat ccgcatatga tcaattcaag gccgaataag aaggctggct ctgcaccttg
     1141 gtgatcaaat aattcgatag cttgtcgtaa taatggcggc atactatcag tagtaggtgt
     1201 ttccctttct tctttagcga cttgatgctc ttgatcttcc aatacgcaac ctaaagtaaa
     1261 atgccccaca gcgctgagtg catataatgc attctctagt gaaaaacctt gttggcataa
     1321 aaaggctaat tgattttcga gagtttcata ctgtttttct gtaggccgtg tacctaaatg
     1381 tacttttgct ccatcgcgat gacttagtaa agcacatcta aaacttttag cgttattacg
     1441 taaaaaatct tgccagcttt ccccttctaa agggcaaaag tgagtatggt gcctatctaa
     1501 catctcaatg gctaaggcgt cgagcaaagc ccgcttattt tttacatgcc aatacaatgt
     1561 aggctgctct acacctagct tctgggcgag tttacgggtt gttaaacctt cgattccgac
     1621 ctcattaagc agctctaatg cgctgttaat cactttactt ttatctaatc tagacatcat
     1681 taattcctaa tttttgttga cactctatca ttgatagagt tattttacca ctccctatca
     1741 gtgatagaga aaagtgaaat gaatagttcg acaaagatcg cattggtaat tacgttactc
     1801 gatgccatgg ggattggcct tatcatgcca gtcttgccaa cgttattacg tgaatttatt
     1861 gcttcggaag atatcgctaa ccactttggc gtattgcttg cactttatgc gttaatgcag
     1921 gttatctttg ctccttggct tggaaaaatg tctgaccgat ttggtcggcg cccagtgctg
     1981 ttgttgtcat taataggcgc atcgctggat tacttattgc tggctttttc aagtgcgctt
     2041 tggatgctgt atttaggccg tttgctttca gggatcacag gagctactgg ggctgtcgcg
     2101 gcatcggtca ttgccgatac cacctcagct tctcaacgcg tgaagtggtt cggttggtta
     2161 ggggcaagtt ttgggcttgg tttaatagcg gggcctatta ttggtggttt tgcaggagag
     2221 atttcaccgc atagtccctt ttttatcgct gcgttgctaa atattgtcgc tttccttgtg
     2281 gttatgtttt ggttccgtga aaccaaaaat acacgtgata atacagatac cgaagtaggg
     2341 gttgagacgc aatcgaattc ggtatacatc actttattta aaacgatgcc cattttgttg
     2401 attatttatt tttcagcgca attgataggc caaattcccg caacggtgtg ggtgctattt
     2461 accgaaaatc gttttggatg gaatagcatg atggttggct tttcattagc gggtcttggt
     2521 cttttacact cagtattcca agcctttgtg gcaggaagaa tagccactaa atggggcgaa
     2581 aaaacggcag tactgctcgg atttattgca gatagtagtg catttgcctt tttagcgttt
     2641 atatctgaag gttggttagt tttccctgtt ttaattttat tggctggtgg tgggatcgct
     2701 ttacctgcat tacagggagt gatgtctatc caaacaaaga gtcatcagca aggtgcttta
     2761 cagggattat tggtgagcct taccaatgca accggtgtta ttggcccatt actgtttgct
     2821 gttatttata atcattcact accaatttgg gatggctgga tttggattat tggtttagcg
     2881 ttttactgta ttattatcct gctatcgatg accttcatgt taacccctca agctcagggg
     2941 agtaaacagg agacaagtgc ttagttattt cgtcaccaaa tgatgttatt ccgcgaaata
     3001 taatgaccct cttgataacc caagagggca tttttta
//
LOCUS       DAPGOF010000291         3027 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.297, whole genome shotgun sequence.
ACCESSION   DAPGOF010000291 DAPGOF010000000
VERSION     DAPGOF010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.297"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            59..1039
                     /gene="virB11"
                     /locus_tag="RHV13_004615"
     CDS             59..1039
                     /gene="virB11"
                     /locus_tag="RHV13_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HDV1244049.1"
                     /translation="MTGIQSVLNIEHVTEISVNKPGTIWFEGKNGWESKDAPDATFDN
                     LMTLAKTLTNLSKIKIPLSHDNPIASVVLPGGERGQIIIPPATENNSVVISIRKPSLT
                     RFTIDDYVRTGRFDNVRIATKHEAILTERQRYLYELSRRPDGQSKAQFLREAVKDRLN
                     FLIVGGTGSGKTTIAKAIADIFPPERRYITVEDVPEMSLPLHPNHIRLFYKKNTVEAK
                     EIIEACMRLKPDHIFLAELRGNEAWSYLEALNTGHEGSISTIHANNTYASFSRLASIV
                     KQSDVGMTVDMDLIMRTIKTSIDVILFFNHTRMTELYYEPEEKNRFLSTM"
     gene            1055..3013
                     /locus_tag="RHV13_004616"
     CDS             1055..3013
                     /locus_tag="RHV13_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HDV1244050.1"
                     /translation="MDAKKTGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLK
                     WDTFHSVISTFSGNPQYKKLVFMAWFGFSVPLIFFAIFMLIVVIGIMPKKVIYGDARL
                     ATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQFIYFAGQQFLILYAPTRSGKG
                     VGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQECFLFAPAGYAETIDQAIKG
                     QIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDK
                     ERFHLEQKAKGHNVPDVLVSISAILKTSVPDGGKDLAAWMGQEIENRSWISDKTKSFF
                     FKFMSAPDRTRGSIETNFSSPLSIFSNPITAEATNFSDFDIRDIRKKPMSIYLGLTPD
                     ALITHEKIVNLFFSLLVNENCRELPEHNPDLKYQCLILLDEFTSMGKSEVIERAVGFT
                     AGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLYYPPKSKNALAKKISEEIG
                     VRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNIAIREII
                     TSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKA
                     GGKKVMVAGGNVITNPDLDNHDKTDVSE"
BASE COUNT          948 a          621 c          632 g          826 t
ORIGIN      
        1 taccagtaat acatcacaac cacgagataa cagtaaaacc gcaagacgat atctggatat
       61 gacgggtatc cagtctgttc ttaatattga acatgtcaca gaaatatctg tgaataaacc
      121 aggaacaata tggtttgaag ggaaaaatgg ctgggaatct aaagatgcgc cagacgcgac
      181 ttttgataat ttaatgacac ttgccaaaac tctgacgaac ttatcaaaga taaaaatacc
      241 acttagccat gataatccca ttgcatcggt agttcttccc ggtggggaac gtggacaaat
      301 catcattcca cctgcaacag aaaataacag cgttgtgata tctatccgta agccatcatt
      361 aacgcgcttt actatagatg attatgtccg aacagggcgt tttgataatg tcagaattgc
      421 gacgaaacac gaagccatcc tgactgaacg gcaacgctac ctgtatgaat tatcccgacg
      481 tccagatggt caatctaaag ctcaattttt aagagaggca gttaaagacc gtcttaattt
      541 tttaatcgta ggtgggactg gctccggtaa gacaactata gcaaaggcaa ttgcagatat
      601 ctttccacct gaacggcgtt atataaccgt tgaagatgta ccggaaatgt ctctgccgct
      661 gcatccaaac cacatccggc ttttctacaa aaaaaatacc gttgaagcga aggaaatcat
      721 tgaagcgtgt atgcgattaa agccagatca catctttctt gctgaactta ggggtaacga
      781 agcatggagt tatctggagg cgcttaatac agggcatgaa ggctcaatca gtaccattca
      841 tgctaataat acttatgcct cattttcacg actggcaagt attgtaaaac aatctgatgt
      901 tggtatgact gttgatatgg acctcatcat gcgcacgata aaaacatcaa ttgatgtcat
      961 tctttttttc aaccatacca gaatgactga actgtattac gaacccgaag aaaaaaatcg
     1021 ctttttgagc acaatgtaaa ttctggagat aactatggat gcaaaaaaga cgggggggtt
     1081 aattctcttc ctgttactgc ttcttgtggg tgtgcttata gcctcaaact atctcggtgg
     1141 atacacagca ttaagatatt catccgttga tatgtctttg ctcaagtggg atacattcca
     1201 ttctgtaatc tcaacatttt ctggcaaccc acaatataaa aaactcgttt ttatggcatg
     1261 gtttggattc agcgttccat taatattctt tgccatattc atgttgattg tcgtgattgg
     1321 gattatgcct aaaaaagtca tctacggtga cgcccggctc gcaactgata tggacctgag
     1381 taaatcaggt tttttcccgg ataaaaaatc accttacaaa catccaccaa tactcatcgg
     1441 taaaatgttc aaggggagat acaaaaaaca atttatttat ttcgccggac aacaattttt
     1501 aattctttat gccccgacac gttctggtaa aggggtgggg attgttatcc ccaactgcgt
     1561 aaactatcca ggaagtatgg tcatactgga tatcaaactt gaaaactggt ttttatcagc
     1621 cggattcagg caaaaagaat tagggcaaga atgctttctc tttgcgccag ccggatacgc
     1681 ggaaactatc gaccaggcca ttaaaggaca gatacgctct caccgctgga atcctcttga
     1741 ttgcgtcagt cgcagtgatt tacttcgtga aacggacctg gcaaaaattg ctgcaatcct
     1801 tatcccggca agtgatgatc ctatatggtc tgattctgca cgaaaccttt ttgtcgggtt
     1861 aggactttac ctgctggaca aggagcgttt tcacctggaa cagaaagcca aaggtcataa
     1921 tgtaccggac gttcttgttt ccatcagcgc catactgaaa acgtcggttc ccgatggcgg
     1981 taaggatctc gccgcatgga tgggacagga aattgaaaac cgtagctgga ttagcgataa
     2041 aacaaaatca ttcttcttta agttcatgtc agcaccagat cgcacaaggg gaagtataga
     2101 aacaaacttt tcatcaccgt tgagcatctt cagcaaccct attactgctg aggcgacgaa
     2161 tttcagtgat tttgatatcc gggatattcg taaaaagccc atgtcaattt atcttggtct
     2221 gacaccggat gctttaatca ctcatgagaa aattgtaaac cttttcttct cattactggt
     2281 taatgaaaac tgtcgtgaac taccggaaca caacccggac ctgaaatacc agtgtcttat
     2341 tctcctggat gaatttacat caatgggtaa atcggaggtc attgaacgcg ctgttggttt
     2401 tacagcaggt tataaccttc gctttatgtt cattcttcag aatgaaggac aagggcaaaa
     2461 aagcgatatg tacggtcagg aaggatggac cacattcact gaaaacagtg ctgttgtgct
     2521 ttactatccg ccgaaatcaa aaaatgctct ggctaaaaag atttcagaag agattggggt
     2581 gcgtgatatg aaaatatcca aacgctcaat atccagtggt ggtggtaagg gaggaagttc
     2641 acgtacacgg aatgatgatg taatagagcg cccggttctt cttcctgaag aaattgtgtc
     2701 tctccgggat aagaagaata aagccagaaa tattgcaatc cgtgaaatta taaccagtga
     2761 atttagccga ccatttattg ccaacaaaat aatctggttt gaagaaccag agtttaaacg
     2821 ccgcgtggat atcgcccgta acaatcatgt tgacattcca aacctgttta cacaagaagt
     2881 catggatgaa atagcaaaaa ttgcagaaat ttatctccct aaagctggag gaaaaaaagt
     2941 catggttgcc ggaggcaatg tgattactaa tccagacctt gataatcacg ataaaacaga
     3001 cgtatctgaa taatcctcgc aataaag
//
LOCUS       DAPGOF010000292         2968 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.298, whole genome shotgun sequence.
ACCESSION   DAPGOF010000292 DAPGOF010000000
VERSION     DAPGOF010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.298"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            173..451
                     /locus_tag="RHV13_004617"
     CDS             173..451
                     /locus_tag="RHV13_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1244051.1"
                     /translation="MAHDTKLHNSDDSAVFASRCGRRFHAFKSDWFQHPPCTEEQAEW
                     IIQCYRRRGYEVKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            453..1502
                     /locus_tag="RHV13_004618"
     CDS             453..1502
                     /locus_tag="RHV13_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HDV1244052.1"
                     /translation="MRVLLRPVLVPELGLVVVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLCHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQQNPPQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEMHGSQVDL
                     IFRFLDHAFATGVLG"
     gene            1515..1889
                     /locus_tag="RHV13_004619"
     CDS             1515..1889
                     /locus_tag="RHV13_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDV1244053.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMHEEQVKK"
     gene            1886..2707
                     /locus_tag="RHV13_004620"
     CDS             1886..2707
                     /locus_tag="RHV13_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HDV1244054.1"
                     /translation="MKLEDLPKYYSPKSPCLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESVVFTNKVQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            2856..2926
                     /locus_tag="RHV13_004621"
     tRNA            2856..2926
                     /locus_tag="RHV13_004621"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2884..2886,aa:Met,seq:cat)
BASE COUNT          738 a          694 c          813 g          723 t
ORIGIN      
        1 ttaaaattta atttatctaa tcgcttttaa taataagcgt tgtgtttatc ccaacaatct
       61 gttgtttgat ttttattcca ttaatgtagg ggctttacac tggaaccagt ttatttatac
      121 tttatacgcc agcctgaaca actggcacct gctgcgccag cagagacaac cgatggcgca
      181 cgataccaaa ttacacaatt ctgatgattc tgccgtcttt gccagcaggt gcggacggcg
      241 ttttcatgca ttcaaatcgg actggttcca gcatccacca tgcactgaag agcaggctga
      301 atggataatt cagtgttacc gcaggcgcgg atacgaggtt aaaaaagccc ttagcctcga
      361 ctaccgtcac tggataatct cagtcagatt gccttactcc gaacgcccac cgcgtccgtc
      421 ccgtacattc cagcaacgca tctggaggta acgtgcgggt attacttcga cctgttctgg
      481 taccggaact cgggctggtg gtcgttaagc cgggccgtga atccatgccg gtattccaca
      541 atacccgggt actggtggag ccggaaccga aaagcatgcg taatctgccg tccggggtcg
      601 ttcctgccgt tcgccagccg ctggtggaag acaaaacatt gctgccgttt ttcagtaacg
      661 cacgggtaat tcgtgctgct ggtggtgctg gtgcattgtc tgactggctg ttgtgccata
      721 ttaaatcctg ccagtggcca cacggcgatt atcatcacag cgaaaccgtt attcaccgtt
      781 atggtaccgg cgcaatggtg ttgtgctggc actgcgacaa ccagctgcgt gaccagacat
      841 ccgaatcact cgagcaactt gctcatcaaa atctgtcagc atggatgatt gacgtcatcg
      901 gtcacgcaat aagcggtacg caggagcgtg aattatctct ggctgaatta tcctggtggg
      961 cggtccgcaa tcaggtggcg gacgcgctac cggaagcggt attacgtcgt tcgctggggt
     1021 tgcgtgcgga aaaaatccgc tcaatgtacc gtgaaagcga catcgtaccg ggagagcaga
     1081 ccgccaccag catactgaaa cagcgcacaa aaaatcttgc gccgctgcct cacgcccacc
     1141 agcaacagaa cccaccacag gaaaagacgg tggtcagcat tgccgttgat cctgagtctc
     1201 cggaatcttt catgaaacga cctaaacgtc gccgctgggt taacgagaaa tacacacgct
     1261 gggtgaagac acagccgtgt gcgtgttgtg gtaagccagc cgacgatccc catcacctga
     1321 ttggtcatgg tcagggcgga atggggacaa aatctcacga tattttcacg ctaccgctgt
     1381 gtcgggagca tcacaacgag cttcatgcgg atccgctggc gttcgaagaa atgcatggtt
     1441 ctcaggttga tttaattttt cgttttcttg atcacgcctt tgcaactggc gtgcttgggt
     1501 aaaagaggtg actgatgctc atagatttgg ttttacctta cccgccgacg gtgaacactt
     1561 actggcgacg ccgtggcagc acatatttta tctcggagga gggaaagcgt tatcgccggg
     1621 ctgtggcgct tattgttcgc cagcagcggc tgaaattaag cctgtccgga aggctggcga
     1681 taaaggtgat tgcagagcca ccggataagc gtcgtcgcga cctggacaat atcctgaaag
     1741 caccgctgga tgcgctgacg catgcgggag tgttaatgga cgatgagcag tttgatgaaa
     1801 tcaatatcgt tcgtggtcag ccagtatctg gtggacgtct gggggtgaag atttacccca
     1861 taatgcatga agagcaggtc aaaaaatgaa actggaagat ttaccgaaat actactcccc
     1921 aaaatcccct tgcctgaccg atgcatcggc ctcaacgtca aaagatgcgc tgagtatcac
     1981 tgatgtgatg gccgcgcagg gcatgacaca gaatcgggct gagatggggt tttctgcgtt
     2041 cctggggaaa atgggcatca gtatgaatga cagggcgcgg gcaacagaat tactggcaga
     2101 ttatgcactc agtcggtgcg atcgtgtggc ggcgttgaga aaacttccgg cagaaataaa
     2161 accggtagtg atgcgcatta tggcttcgta cgcttttgag gattatgccc gcagcgcagc
     2221 gagtaaaaag cagtgccctt gttgctatgg ggaaaaattt attgaaagcg tagtttttac
     2281 aaacaaggtc cagtatccgg atggtaagcc gccggtatgg gcaaagtgta cgaaaggtgt
     2341 gtatccgtct tactgggaag aatggaaaaa agtcagggag gtggtaaaag ttgcctgtcc
     2401 ggagtgtggc ggaaagggtg aggtttccac cgcctgtaag gattgccgtg ggcgtggtgt
     2461 cgccattcac cgtgaagagt cggtaaaacg tggtatgcct gttatcagag actgccagcg
     2521 ttgtggtggt cgtggctatg aaagactacc atcaacggag gcatttaatg ctatatgcga
     2581 ggtgacaaac cagataacac gcgcgtcatg ggaaaaaaca gttaagaaat tctatgatgc
     2641 gctggtgacc cggtttgata ttgaagaagc atgggctgag cggcagttaa aaaaggtaac
     2701 taggtaacaa ggttgatttt tccggaatct gtggtaaatt cgtcataatg atgggctttt
     2761 tatgcctgac gttagaagag tttctacaac ccgccgccga gcgggttttt tattgcggaa
     2821 ttaattacgg accgttatta ttctgctcac ggccctttag ctcagtggtg agagcgagcg
     2881 actcataatc gccaggtcgc tggttcaaat ccagtacagc gcgccatatt cattcttcca
     2941 gattccttcc ggcagagcct tatactga
//
LOCUS       DAPGOF010000293         2960 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.299, whole genome shotgun sequence.
ACCESSION   DAPGOF010000293 DAPGOF010000000
VERSION     DAPGOF010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.299"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            44..676
                     /gene="comR"
                     /locus_tag="RHV13_004622"
     CDS             44..676
                     /gene="comR"
                     /locus_tag="RHV13_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HDV1244055.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(738..1277)
                     /locus_tag="RHV13_004623"
     CDS             complement(738..1277)
                     /locus_tag="RHV13_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDV1244056.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1504..2808)
                     /gene="ndh"
                     /locus_tag="RHV13_004624"
     CDS             complement(1504..2808)
                     /gene="ndh"
                     /locus_tag="RHV13_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HDV1244057.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
BASE COUNT          723 a          797 c          716 g          724 t
ORIGIN      
        1 ttataataac ctttcattca cagtcaccag gtataacggc atcatggcaa ctgactcaac
       61 acaatgtgta aaaaaaagcc gtggccgccc aaaagtgttc gacagggatg ccgcgcttga
      121 taaggccatg aaattgttct ggcaacacgg ttatgaagcg acttctcttg cggacctcgt
      181 cgaagcgacc ggagccaaag cgcccacgct atacgcggaa tttaccaaca aagaggggtt
      241 atttcgtgcc gttctcgacc gctatatcga tcgttttgcc gctaagcatg aagcacagct
      301 gttttgtgaa gagaaaagcg tggagtctgc gctggctgac tattttgctg ccatcgccaa
      361 ctgctttacc agcaaagaca ccccggcagg ctgcttcatg atcaacaact gcaccaccct
      421 ctccccagat tcaggagata tcgccaatac gttgaaatca cgccatgcga tgcaagagcg
      481 cactttgcag cagtttttat gtcaacgaca agcgcgcggg gaaatcccga cccactgtga
      541 cgtgacacat ctggcagaat tccttaattg cattattcag gggatgtcga tcagcgcacg
      601 cgaaggtgca tcgctggaaa aactgatgca gattgccgga acgactttgc gtttatggcc
      661 cgaactggtg aaataagtcg caaaaaaagc ccctcagcct gagcgaatga ggggccaaat
      721 tgcagagtac agccttgtta tactttgtta ttcaaaatca gttgcccgtt actatctagc
      781 gggatctgcg tacccggatc gcggtccatg cggattttgc cctgctgatc gccaatctta
      841 taggtcacat cataaccgag cattttttct gacttgtcat acaccgtttt acaacgctgt
      901 tgcgtagtcg tgtaagtatc gctttcctgg agagagccct ggatctggtt accggcatat
      961 ccaccaccca gcgcccccac aacagtggcg acatctttac cacgaccacc accaaactga
     1021 tgccctatca cgccgccagc aacagcgccg agcaccgacc cggtaatgcg attttcatcc
     1081 tgcaccggtc gacgatgggt cactgtgacg ttgcgacact cctgacgcgg tgttttaacc
     1141 gtttccttga ttggggttgc agaaacaacc tgagcgtatt gcgggccccg ttcaaacacg
     1201 ttcagactgg ccactgccgc tacgcccagc gcagctgcga caccaatccc gatacccgcc
     1261 aacattgatt tattcacggg aacacctcct tctttgcagt tatgccaatc atgcaagcga
     1321 aggagaaaag cagctatcag acagaagatg aaaagcgcgg gaatggctgg aaatacttaa
     1381 ggattcggag gagtctgaag gaatgcctga tgcgacgctt aacgcgtctt atcaggccta
     1441 ccgctcgtac catttgtcca gggcggatga ggcgtttatg ccacatccgc cagtgtacgt
     1501 cgattaatgc aacttcaaac gcggacggat aacgcggtta atactcccca ccagcatcat
     1561 taatccggtt ttaaagtaac catgcagcgc aatctgatgc attcggtata gcgagatata
     1621 tacaaagcgc gcaattcgtc cttcaatcat cattgagccg cgcgtcaggt tacccatcag
     1681 gctaccgacg gtggagaagt tcgacagcga taccagcgaa ccatgatctt tatactgata
     1741 atttttcagc ggcttaccgt tcatctgcgc cagtatgttg ttcattgcgc aagtcgccat
     1801 ctggtgtgca gcctgagcac gcggcggaac aaagccccct tccggacgcg ggcatgacgc
     1861 gcagtcgcca atagcgtaaa tgtctggatc gcgggtggtt tgcagcgtcg gttccaccac
     1921 cagctggttg atacggttag tttcaagacc accgatatct ttcaggaagt ctggcgcttt
     1981 gatcccggct gcccacacca tcagatcagc ctcaatatat tcgccatctt tagtgtgcag
     2041 gccgccttca tcagcactgg tgaccatggt ttgcgtcagc acgcgaacgc caagtttcgt
     2101 tagctcgttg tgggccgcag cagagatacg tggcggtaat gcaggcaaaa tacgttctcc
     2161 cgcttctacc agcgttacgt tcagggcttc gttggtcagg cctttgtaac cgtagctgtg
     2221 cagttgcttg accgcgttgt gcaattcagc ggagagttct acacccgtcg cgccgccgcc
     2281 gacaatcgca atgttcactt tgccatttgc gcccaggttg gcggagtatt tcaggaagag
     2341 attcagcatc tcctggtgaa agcgacgcgc ctggtgcggg ttatcgagga aaatgcagtt
     2401 ctctttgaca cctggcgtat tgaaatcgtt agaggtgcta cccagcgcca ttaccagggt
     2461 gtcataggcg attttacgtt ccggaaccag cagttcacct ttctcgtcgc gcagttctgc
     2521 aatagtgatt gttttcgctt cacgatcaat atcaatgacg gaacccagct ggaactggaa
     2581 accatgattg cgcgcatggg ccagatagct caacgcatcg acgccttcat caagcgagcc
     2641 agtcgccact tcgtgcagca gcggtttcca caggtggctg tggttacgat cgaccagcgt
     2701 aattttggct tttttcttgc gtcccagctt atgccccagc tgtgttgcca tttccagccc
     2761 accagcaccg ccgccgacaa tcacaatttt tttcaatggc gtagtcaacg tgaccccctt
     2821 aaatttatta accaattgtt aattaatggt tattaacata gctcttaatt aacaacaggt
     2881 tacagcaatg aaaatgttct tcgctattga gaataacatg aatggtgcat tggtcatacc
     2941 aaaattgatg ttaatcaagt
//
LOCUS       DAPGOF010000294         2916 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.300, whole genome shotgun sequence.
ACCESSION   DAPGOF010000294 DAPGOF010000000
VERSION     DAPGOF010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.300"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1579
                     /locus_tag="RHV13_004625"
     CDS             <1..1579
                     /locus_tag="RHV13_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HDV1244058.1"
                     /translation="KINANELAAERDAQKATEYAKKIRNNPVIQDSTKQWINTTPIIE
                     KQVKQSAPPCPIVINTRSDIELRQIAQRITQTCHIPVRISADVWTYLNGTGTGSTQQM
                     TGSIPAPDANGMVPLASVGSQPVKVSSGGTSLPELNISGLPALLQTVSSRLGISWKYD
                     KGRITFFYLETRSFPITYMDSNVAYNSKVVSGTMSSSGSTGSTSSGGMTGDASNTQTT
                     TVEMKSSLYNDLKSEVSSMLTPGTGRMYLSTGSLTVTDTPDVLDSVQEIVNKRNNEMS
                     RQVVLNVEILSIKKSSQEQAGIDWNAVFNDGHLGLSLGGSFGDAAENVITSGVSIVDG
                     KLAGSKAFLKALSSQGSVSVVTQNSAVTKNLTPVPMQIANQQGFIESVTTDSTANVGS
                     STSLNAATITTGFNMTLLPYIQPDSQNLQLLFSMSLSDKPTFEVFESGGSKAQTPNVD
                     LKTINQTIDLKSGQTVILSGFQQSNRKTSKQGVITPSFFGLGGGVNSEDGDTILVVLI
                     TPNVLNSGSSAVIKNVN"
     gene            1618..>2916
                     /gene="pilO2"
                     /locus_tag="RHV13_004626"
     CDS             1618..>2916
                     /gene="pilO2"
                     /locus_tag="RHV13_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HDV1244059.1"
                     /translation="MEVTLSENNQNNRNFTSVIKNKRAFFSGLDWKTLPSEEKNARTF
                     ARKNDAEYFLSCQYQDSENETKTMVAFIRKEDLPTGASSFWSLALMIKPLIEPDGYAI
                     CELGDLYGFVSCVNNVLVNDVVGNKSQIMSALTTFLEFNETPEPGWKLYQPESWDISQ
                     ALPSLTLSALIDVKKPPKEAAFTRVSRKRQFMIYGGSAILAILLWNGITMYQEYREKE
                     AAAEAARLRLAKEMADKQAIQIAPPWQHLPEIKPFIDKCIDKWDALPLSIAGWRFDLA
                     ECSTSGNDGLLRTSYKELSGVTVEDFSTRIREIFQGTTTATFVLPEGSAGGFSLPVSF
                     DVSPDPITPDTLPQATDIQERLTTFAQKMRLKLTWQEIENTKTDEEGRPIILPWNEYE
                     LMIQTSTPPSILFANFHEPAVRFQYAGIKLEEGRLNYEIKG"
BASE COUNT          884 a          691 c          655 g          686 t
ORIGIN      
        1 aaaaatcaat gctaatgaac ttgcagccga aagagacgca caaaaagcga ctgaatacgc
       61 gaaaaaaatt cgtaataacc cggttatcca ggactcaaca aaacaatgga ttaatacaac
      121 gccaattatt gaaaagcagg taaagcaatc agcgccgccc tgcccgattg tgattaatac
      181 tcgttcagat attgagctac gccagatcgc acaacgcatc acacaaacct gtcatatccc
      241 ggtacgaatc agcgccgatg tctggaccta tctgaacggt acaggcactg gctcaacaca
      301 acaaatgaca ggatcaattc cagcaccgga tgcaaatgga atggttcctc tggcgtcagt
      361 aggcagtcag ccagtaaaag tctcttctgg cggtacatca ctcccggaac tgaatatctc
      421 cgggcttcct gccctactgc aaaccgtatc ttcgcggctg ggaatatcct ggaaatacga
      481 taaaggtcgt atcacattct tctatctgga aacgcgctca ttcccgatca catatatgga
      541 cagcaatgtg gcatacaact caaaagttgt gagcggcaca atgtcttcca gtggcagtac
      601 cggctctacc agttctggcg gtatgactgg cgacgcatca aatacgcaaa ctacaaccgt
      661 ggaaatgaaa tcatcgctgt acaacgacct gaaaagcgaa gtttcctcaa tgctgacacc
      721 aggtacaggg cgcatgtatc tgtccactgg ttcactgaca gtaacagata ccccggatgt
      781 actggacagc gttcaggaga ttgtgaacaa gcgcaataat gaaatgagtc gtcaggttgt
      841 gctgaacgtt gaaatcctga gtatcaagaa atcaagccag gaacaggcgg gaatcgactg
      901 gaacgcagta tttaatgatg gtcatcttgg tctgagtctt ggtggctcat ttggtgatgc
      961 cgccgagaat gttattacca gtggtgtatc catcgtggac ggtaagctgg ctggctcaaa
     1021 agcatttctg aaagccctgt cgtcacaagg cagcgtcagt gtcgtaacgc aaaactccgc
     1081 tgtgactaaa aacctgacgc ctgtccctat gcaaattgcc aatcagcagg gcttcattga
     1141 aagtgtcaca accgattcta ccgccaatgt gggatcatcc acgtcactga atgcggcaac
     1201 aataacgacc ggattcaata tgactctgct tccatacatt cagccggatt cacaaaacct
     1261 gcaattgctg ttctcaatga gtctgtctga caaacctact tttgaggtgt tcgaaagtgg
     1321 cggcagtaaa gcacaaacac cgaatgtgga tttgaaaaca attaatcaga ctattgatct
     1381 gaaatcaggc cagacagtca ttctgagtgg attccagcaa tcaaaccgga aaaccagtaa
     1441 gcaaggtgta ataaccccct ccttctttgg acttgggggt ggcgttaaca gcgaagacgg
     1501 cgacacgatt ctggtcgttt tgattacacc aaatgtgctt aattccggct ccagtgcagt
     1561 aataaaaaat gtgaactaat taactgaaaa ctccctgaaa aaacacaggg agtttttttg
     1621 gaggtcactt tgtctgagaa taatcagaat aaccggaatt tcacttcggt tattaaaaac
     1681 aagcgtgctt ttttctccgg tctggactgg aaaacgcttc cttcagaaga aaagaatgcg
     1741 cgtacattcg caagaaaaaa cgacgctgaa tattttctct cctgtcagta tcaggattca
     1801 gaaaatgaaa ccaaaacgat ggtggcattt atcagaaaag aggatcttcc cacaggggcg
     1861 tcctcattct ggtcactggc actgatgata aaacccctca tcgaacctga cggatacgct
     1921 atctgcgaac tcggtgatct ttatggcttt gtttcctgcg tcaataacgt tctcgtcaac
     1981 gatgttgtag gtaacaaaag tcagatcatg agtgctctga caacatttct tgagtttaat
     2041 gaaacaccag aacccggctg gaaactatac cagcctgaat catgggatat cagccaggca
     2101 ctcccctcac tcactctgtc tgcgttgatt gacgtaaaaa aaccgccgaa agaggcggcg
     2161 tttactcgcg tatcccgaaa acgtcagttc atgatttatg gcggaagtgc cattctggcc
     2221 atactgctct ggaacgggat caccatgtac caggaatacc gggaaaaaga agctgcggca
     2281 gaagctgcac gtcttcgtct ggcaaaagaa atggcagaca aacaggcaat acagattgcc
     2341 ccaccgtggc aacatttacc tgaaataaaa ccatttattg ataaatgcat tgataaatgg
     2401 gatgcactgc ccttatcgat cgccggatgg cgttttgacc ttgctgaatg cagcacatcc
     2461 ggtaatgacg ggctgttacg cacatcatat aaggaactga gcggcgttac ggtcgaggac
     2521 ttcagcacac ggatacgaga aatattccag gggacaacca cagcaacttt tgtattgcca
     2581 gaaggcagcg ccgggggatt ttctcttcct gtcagttttg acgtttcgcc tgatccaatt
     2641 accccggaca cattgccaca ggcaacagat attcaggaac gcctgacaac atttgcacaa
     2701 aaaatgaggt taaaactcac ctggcaggaa attgaaaaca caaaaacaga tgaagaaggc
     2761 cgtccaataa ttctcccgtg gaacgagtat gaattaatga ttcagaccag caccccgcct
     2821 tcaatacttt tcgctaactt tcatgaacct gccgttcgct tccagtatgc aggaataaaa
     2881 ctggaagaag gaagactgaa ttatgagatt aaaggt
//
LOCUS       DAPGOF010000295         2889 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.301, whole genome shotgun sequence.
ACCESSION   DAPGOF010000295 DAPGOF010000000
VERSION     DAPGOF010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.301"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(220..2610)
                     /locus_tag="RHV13_004627"
     CDS             complement(220..2610)
                     /locus_tag="RHV13_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain repeat protein"
                     /protein_id="HDV1244060.1"
                     /translation="MKIKLALLIAFAGFSAGASAEIAEFSFKDTLNVKKTVSPASVWI
                     NPVTSFDVAVISGLDRYITLSLLKSDGSLIWSTKSSLVTVDDRTTSSTGFDYYGKTLT
                     VPAMGEDSFTLREVITDLQGKEVSRQDYPLAIDRTPPATGTISYTRNGWNFGSEAIFT
                     SVPAGMQYASVQALVFNGLSDKGSGLANAEYFITDAAGVERKKPAEINTVEGSVTVQV
                     ADASSNALAPENRSEYKVGIYLYDKAGNRSELSRRSVIDRVKPDDIIQVQDATTGSWV
                     TYQSGMTVFQNPISVRVLRKKSDFTAVNGSKYGWADSNFQTSDSTYNIYTFKYIYPNV
                     GDTYHEFQTLAGGVRRIHHNSLNFTPAPAMEIAPKIVAKEMYRSDTSEWLTQASISVK
                     TATISRIKVTAEPRPYVQKFRTVKNAAWFCTIPIGQSSCEMTVNFNYTSDKGFEYLHL
                     YSGKDGDSIFDALAGNFTVIWDNNPPVVNVAQVNKASKTITMTATDNDRVNAWNISYW
                     DTKVFEATLKNARGEPFTLKPVTVSESDYKTKNATFSYAGLPDGDYTVVSVSATDLVG
                     NTGTKTLNAPLKIDSTLPVIAFSFNGADAEGKLVKGLENLRISVTDASGDASLTSLQL
                     AGGPNSEKVTLAFTPLSKDVFIPEYPRIFPNTDESGQMYHLEALAIDESGNRTTKTLN
                     FTYQPANLIMLDNLKTLATAVALKATDNTPLAIIRTSVLRRQDGSIITGQLNGTLTVQ
                     KNAQFGVTVSGVTVQPGETKSLSLDLGNGEERTYPVTPAVSGQSGTATFAIEFPQT"
     gene            complement(2683..2850)
                     /locus_tag="RHV13_004628"
     CDS             complement(2683..2850)
                     /locus_tag="RHV13_004628"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1244061.1"
                     /translation="MKPMLKKDFFSAIENLLKAGFQPRFYTVNSGRIGLITFTDKNGT
                     KQVPPGRKEKH"
BASE COUNT          681 a          675 c          709 g          824 t
ORIGIN      
        1 atttaaacgc gggcattgtt ccggagtgat taaacggttt agcatcatag attaaaacca
       61 aaaggaaggc gtccttcccg tctcgggaat attactttcc cgtttgcaat ttaagagcca
      121 cttaatgaga aagtaataat taagtcatgc tcaactctac atattacatt gaggatggcg
      181 taacagggta cggcatacta ccgcaccctt ctgaagagct tatgtttgag ggaattctat
      241 agcaaatgtg gccgtgccag attgaccact tacagcggga gtaacgggat acgtgcgctc
      301 ttcaccatta ccaagatcca gtgacagcga tttagtttca ccaggctgga ccgtaactcc
      361 ggaaaccgta acgccgaact gggcattttt ctgaacagtc agggttccgt ttaactgtcc
      421 ggtaatgata gaaccgtcct gacgccgcaa cacactggtt cggatgatgg ccagcggcgt
      481 gttgtccgtt gctttcagtg ctacggctgt ggccagcgtc ttgagattat ccagcataat
      541 cagattcgca ggctggtagg taaaattaag cgttttagtg gtccggttac ctgattcgtc
      601 aatagccagt gcctccagat gatacatctg tcccgattcg tcagtgttgg ggaagattct
      661 ggggtattcc ggaataaaga catccttgga taacggcgtg aatgccaggg tgaccttttc
      721 agagttaggt ccaccagcga gctgaagcga tgtcagggaa gcatccccag aggcatcagt
      781 aacgcttatg cgaaggttct caagcccttt aaccagtttt ccttctgcgt ctgcgccatt
      841 aaaactgaac gcgataaccg gaagcgttga gtcaattttc agcggtgcat taagcgtttt
      901 ggttccggtg tttccaacaa gatccgtggc agagacgcta accaccgtat aatccccgtc
      961 cggtagacca gcgtatgaga aggtggcgtt tttggtttta taatcgcttt cactgacggt
     1021 cacaggcttc agcgtgaaag gttccccccg ggcattttta agggtggctt cgaaaacttt
     1081 ggtatcccag tagctgatgt tccaggcgtt gacgcgatca ttatcggtgg ccgtcatagt
     1141 gattgtcttg gaggccttgt ttacctgagc gacattaacc accggtgggt tgttatccca
     1201 gataaccgta aaattaccag caagcgcatc gaatatgctg tcaccatctt ttcccgagta
     1261 aaggtgcaga tactcaaatc ctttgtcact ggtgtagttg aagttaaccg tcatctcgca
     1321 tgaactttgt ccgataggta tagtgcagaa ccaggctgca ttttttaccg ttctgaactt
     1381 ttgcacgtat ggtcggggtt cagcggttac ctttatgcgg ctaatggtgg cggttttaac
     1441 gctgattgag gcctgagtta accactcact ggtatcactc cggtacatct ctttagccac
     1501 tatcttcggg gctatttcca tcgccggggc tggagtgaag ttgagggagt tatgatgaat
     1561 tcgtcttact cctcctgcca gagtctggaa ttcatgataa gtatccccca cattgggata
     1621 tatgtatttg aaagtataaa tattataagt gctatcggac gtctggaaat tcgagtccgc
     1681 ccagccatat ttggaaccgt taacagcagt aaagtcactt tttttcctta atacccgtac
     1741 ggaaataggg ttctggaaga cggtcatgcc agactgataa gtgacccatg atccggtagt
     1801 ggcgtcctgc acctggatga tatcgtcagg cttaacccga tcaattacgc ttcggcggct
     1861 tagttcgctc ctgtttcccg ctttgtcata aaggtagatc cccactttat attcagagcg
     1921 gttttccggt gccagggcat tgctgcttgc gtcggcgact tgaacggtta cgctaccttc
     1981 cactgtatta atttccgctg gctttttacg ctccactcca gcggcatcgg ttatgaaata
     2041 ttcagcatta gcaagcccgg aacctttatc actcagtcca ttaaagacca gtgcctggac
     2101 gctggcgtac tgcataccgg ctggtactga agtgaagatc gcttcgctgc caaagttcca
     2161 cccgtttctc gtataactta tggtacccgt tgccggaggc gttcggtcaa ttgcaagcgg
     2221 gtaatcctgc cgggagacct ccttcccttg caagtcggta atcacctctc tgagggtgaa
     2281 gctatcttct cccatcgctg gtacggtcag agtcttgccg taataatcaa agcctgtcga
     2341 agatgtggta cggtcatcta cggtaaccag gctgcttttc gtgctccaga tgaggctccc
     2401 gtcacttttc agcagactga gcgtaatata acggtccaga ccggaaataa cggcaacgtc
     2461 aaagctcgtt accggattaa tccatacgga tgcaggcgat acggtctttt taacgttcag
     2521 ggtatcttta aagctgaatt ctgcgatttc agcggaagct cctgcgctga aacctgcaaa
     2581 tgcaataagc agggctagtt tgattttcat gggttgtctc ctgatcaggt tccccatgat
     2641 actttttcag tgttcgagat cttttcggga aaatagctaa ttttaatgtt tttcttttct
     2701 gcccggcggg acctgttttg ttccattttt atcagtgaat gtaatgagac cgattcgtcc
     2761 actgttaaca gtgtaaaagc gaggttgaaa gccagctttg agaaggtttt caatagctga
     2821 aaaaaaatct tttttgagca tcggtttcaa gatggttttc cttttatgcc cggtgccgcc
     2881 gggacggtg
//
LOCUS       DAPGOF010000296         2861 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.302, whole genome shotgun sequence.
ACCESSION   DAPGOF010000296 DAPGOF010000000
VERSION     DAPGOF010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.302"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(33..1199)
                     /gene="ugd"
                     /locus_tag="RHV13_004629"
     CDS             complement(33..1199)
                     /gene="ugd"
                     /locus_tag="RHV13_004629"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HDV1244062.1"
                     /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVDMLNKKQSP
                     IVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIR
                     DVTEINPNAVMVIKSTIPVGFTKSIKERLGIDNLIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(1448..2854)
                     /gene="gndA"
                     /locus_tag="RHV13_004630"
     CDS             complement(1448..2854)
                     /gene="gndA"
                     /locus_tag="RHV13_004630"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HDV1244063.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLTGPKAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFSAAVAYYDSYRSAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
BASE COUNT          739 a          686 c          655 g          781 t
ORIGIN      
        1 atcagggcga aaatcgccct gataacagga tgttagtcac tgccaaagag atcgcgggtg
       61 tagactttgt ctgccacatc cttaagctct tctgccatac ggttggaaat aatgacgtcg
      121 gcttgttgtt tgaaggtggc gagatctcgt tccaggcgag agttgaagaa tgagtcttct
      181 ttcatcaccg gctcgtatat gatcacttca acgcctttcg ccttgatacg tttcataatc
      241 ccctgaatgg aagacgcacg gaagttatct gacccgctct tcataatcag acgataaata
      301 cccaccactt gcggtttgcg tgacaaaatg gcatcggcaa taaagtcttt acgcgtgcgg
      361 ttagcatcga caattgccga aatcaggtta ttcggcacag actggtagtt cgccagtaac
      421 tgcttggtat ctttcggcag acaataacca ccataaccaa acgacggatt gttgtaatgg
      481 ttgccaattc gtgggtcgag acaaacgcct tcgattattt gacgggaatt cagacctaaa
      541 ctttctgcgt agctatccag ttcgttaaag tacgccacgc gcatcgccag gtaggtgttt
      601 gcaaaaagtt taatcgcttc tgcttcagtg gagtcggtaa acagggtcgg gatattttgc
      661 ttaatcgctc cttcctgtaa caacgcagca aaacgttctg cgcgttctga acgctcacca
      721 atgacaatac gtgaaggatg gagattatcg taaagggctt taccttcacg gagaaattcc
      781 ggagagaaaa taaggttatc gatacccaga cgttctttaa ttgatttggt gaaacctacc
      841 ggaattgtag atttaatcac cattaccgcg ttgggattaa tttctgtcac atcacggatg
      901 accgcttcca cgcttgaggt attaaaataa tttgttttcg ggtcataatc ggtaggcgtg
      961 gcaataataa cgtaatcggc atttttatac gcgtcatact tatctgtcgt agcgcggaaa
     1021 ttgagatctt tagtcgccag atactcttca atctccttat caacaatcgg tgactgcttc
     1081 ttgttaagca tgtcaacttt ggcctgaacg atatccaatg caactacttc gtgattttgc
     1141 gcaatcagaa taccatttga aagaccaaca taacctgttc ctgaaattgt tattttcatt
     1201 agctctgact tcttccggtt aaacatttag agtggtcatt aatccaccac cacatcttca
     1261 tctactgcgg gatttttaac gccgatgcgg atgaactgtc aaggcagcga tcctgctgtg
     1321 cgactctgta ttatatcgct tttttaacca taaattataa aaaaaggcca taacctgccg
     1381 ctttgtataa taaaaaagcc cggagggttc ctccgggcct tgcattgatt aattgattta
     1441 aatcagatta atccagccat tcggtatgga acacaccttc tttatcaatg cgcttataag
     1501 tatgcgcacc gaaatagtca cgttgtgcct ggatcaggtt cgcaggcaga acagcggaac
     1561 ggtagctgtc ataataggca actgccgcag agaaggtcgg aaccgggata ccgttctgta
     1621 ccgcataagc aacgacatca cgcagcgcct gctggtagtc atcggcaatt tgcttaaagt
     1681 acggagccag cagcaggtta gcgatttgcg gattttcggc ataggcatcg gtgattttct
     1741 gcaggaactg cgcacggatg atgcagccag cacggaaaat cttcgcgatt tcgccatagt
     1801 tcagatccca gttgtactct tcagacgctg cgcgtagctg agagaagccc tgggcgtaag
     1861 aaacgatttt gcccagatac agcgcacgac gaactttttc gatgaactca gccttatcgc
     1921 ctgctggctg cgctttcggg ccagtgagaa ctttagacgc ggcaacgcgc tgatctttca
     1981 gagaagagat ataacgtgca aacacagact cggtaatcag cgacagcggt tcgccgagat
     2041 ccagcgcact ctggctggtc catttgcccg tacctttgtt tgctgcttca tccagaatca
     2101 catcaaccag gtagttaccg tcttcatctt ttttggtgaa gatatctttg gtgatgtcga
     2161 tcaggtagct gctcagttca ccgttattcc attcggtaaa ggtctgtgcc agttcttcgt
     2221 tggaaagatt caggccacct ttaagcagag aataggcttc agcaatcagc tgcatatcgc
     2281 cgtattcaat accgttgtga accatcttca catagtgacc tgcaccatcg gcaccaatat
     2341 aggtaacgca tggctcgccg tcttcagcca ctgcggcgat tttggtcagg atcggagcaa
     2401 ccagttcata ggcttctttc tgcccaccag gcataatgga aggacctttc agcgcacctt
     2461 cttcaccgcc ggagacaccg gtaccgataa agttaaagcc ttctgcagaa agctcacggt
     2521 tacgacgaat ggtgtcctgg aagaaggtgt taccaccatc aatgatgatg tcacctttat
     2581 cgaggtatgg cttgagggaa tcaatagcag catccgtgcc tgcacctgct ttcaccatta
     2641 acaggatgcg acgaggcgtt tccagagatt caacaaactc tttcaccgta tagtaaggaa
     2701 ccagtttctt gcctggattt tcggcaataa cttcttccgt cttttcacgg gaacggttga
     2761 aaatagagac ggtataacca cggctttcga tgttgagcgc aaggttgcgc cccatcactg
     2821 ccataccgac tacgccaatc tgttgctttg acattgttta c
//
LOCUS       DAPGOF010000297         2853 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.303, whole genome shotgun sequence.
ACCESSION   DAPGOF010000297 DAPGOF010000000
VERSION     DAPGOF010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.303"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..206)
                     /locus_tag="RHV13_004631"
     CDS             complement(<1..206)
                     /locus_tag="RHV13_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HDV1244064.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCGLTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECADLQETTNAI"
     gene            complement(219..2669)
                     /locus_tag="RHV13_004632"
     CDS             complement(219..2669)
                     /locus_tag="RHV13_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDV1244065.1"
                     /translation="MLRTTRWVAAIIFLYSFPGYAEETFDTHFMIGGMRGEKVSEYRF
                     DNKQPLPGNYELDFYVNHQWRGKKDITIPESPAKPCLPKVLLTTLGVKTDNLNTEDNC
                     ILLDKAVHGGQYQWDISEHRLNLTVPQAYINELERGYVPPESWDRGIDAFYTSYNLSQ
                     YRSYDSNNNSHTASYGRFNSGLNLFSWQLHSDASYSKPDDMKGTWQSNTLYLERGWSQ
                     ILSTVQIGENYTSSLIFDSLRFSGIRLFRDMQMLPDSMQSFTPLVQGVAQSNALITVS
                     QNGYIIYQKEVPPGPFTIADLQLSGSGSDLDVSIKEADGSVRSFLVPYSSVPNMLQPG
                     VSNFDFIAGRSQIYGVKNQEDFLEANYIYGLNNLLTLYGGTILSDNYNAITLGNGWNT
                     PLGAISFDATRSSSKLNNDTRHEGTSYQVAYNKYLLQTATHFSVAAWRYASQDYRTFS
                     DHLYENDKINHQSDYDDFYDIGRKNSLSANIMQPLSNNLGNVSLSALWRNYWGRSGNA
                     KDYQFSYSNNWQHISYTFSASQSYDENDKEEERFNLFISIPFYWGDDIAKTRHQINLS
                     NSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYVNQQQQNNDTSLGTNLSWRTPIATID
                     GSYSHSKNAWQSGGSISSGLVVWPGGINITNQLSDTFAILDAPGLEGAHINGQKYNRT
                     NNKGQVVYDLMIPHRENHLVLDTANSESETELQGNRQIIAPYRGAVSYVQFTTDQRKP
                     WYIQALRPDVSPLTFGYDVLDLQENNIGVVGQGSRLFIRVDEIPTGIKVALNDEQNLF
                     CTITFQHVIDENKSYICQ"
     gene            complement(2682..>2853)
                     /locus_tag="RHV13_004633"
     CDS             complement(2682..>2853)
                     /locus_tag="RHV13_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDV1244066.1"
                     /translation="TVTTIKAQNVKVNNESILLPPFSNNDITLKNNHASEYELTVVDD
                     YGNNIHSNIAAR"
BASE COUNT          796 a          579 c          578 g          900 t
ORIGIN      
        1 ttaatggcgt tggtcgtctc ttgcaagtct gcacactcaa tgatatttcc ggtgttttca
       61 attttttcgg cggtgagatt acctttcata ctcagtggct tgagtatata tgttccacgt
      121 tgttccccga cattggtcaa tccacaggga gcactttgca caataaaaga atatagactc
      181 aaggtgatta ataaaagcca cttcatagta aactcctttt actggcatat atatgatttg
      241 ttttcatcga taacgtgttg aaaagtaata gtacagaata agttctgttc atcattgaga
      301 gcaactttta tgccagtggg aatttcatct acgcgaataa aaaggcgact accctggcca
      361 acgactccaa tattgttttc ctggagatcc agtacgtcat agccaaaagt taatggcgaa
      421 acatcgggac gcagtgcctg tatataccag ggcttacgtt ggtcagttgt aaactgcaca
      481 taagaaactg ctccacggta aggcgcaata atttgacgat tgccctgcaa ttctgtttca
      541 ctttcactgt ttgctgtatc aagtaccaga tggttttcac gatgaggtat catcaggtcg
      601 taaacaacct ggcctttgtt gtttgttcgg ttgtattttt gtccattaat atgcgcgcct
      661 tctaacccag gcgcatccag aattgcaaat gtatcggata gctggttagt gatattaata
      721 ccaccgggcc agacaactaa tccactacta atacttccac cactttgcca ggcgttttta
      781 gaatggctat agctgccatc tattgtggcg attggagttc tccagcttaa attcgtacca
      841 agcgaggtat cattattttg ttgttgctga ttaacatata taccataatt taactgatca
      901 tgttcaccgg ctatgccagt aattccagta ttgttggagg aatagccatc tttggaaaat
      961 gaggtagaat tcgataagtt aatttggtga cgtgttttgg caatatcatc accccagtag
     1021 aaaggaatgg agataaacag attaaaacgc tcctcttctt tatcgttttc atcataggat
     1081 tggctggcag agaaagtata actaatgtgt tgccagttat tggagtaact gaattggtaa
     1141 tctttagcat ttccacttcg cccccagtaa ttccgccaaa gtgcacttaa tgatacgttt
     1201 cccagattat tggataaagg ttgcattata ttggcagaaa ggctattttt tctgccaata
     1261 tcataaaaat catcatagtc actctgatga tttattttat cattttcgta aagatggtcg
     1321 ctgaatgtcc tgtaatcctg cgaagcataa cgccaggcgg ccacgctaaa gtgagtggcc
     1381 gtttgcaata gatatttatt ataggcgacc tgatagctcg taccttcgtg tctggtatca
     1441 ttatttaact tactactcga tcgggtagca tcaaaagaga ttgctcccag cggtgtattc
     1501 caaccatttc ccagagttat agcattatag ttatcggata aaatcgtacc gccataaagt
     1561 gtcaaaagat tgttgaggcc atatatataa tttgcctcga gaaagtcctc ctgattcttg
     1621 acaccatata tctgactacg tccggcgata aaatcgaaat ttgaaacacc aggttgcagc
     1681 atattgggca cggaagagta gggcaccaaa aaggaacgaa cactaccatc tgcttcctta
     1741 atgctgacat cgagatcaga gccgctgccc gaaagttgca aatctgcgat agtaaatggt
     1801 cccgggggaa cctctttttg gtaaatgatg tagccatttt gcgaaaccgt tatcaaggca
     1861 ttactttgcg caacaccttg tactaaaggt gtaaagctct gcatagaatc aggtagcatt
     1921 tgcatatcgc gaaataagcg tattccgcta aatctcagag agtcgaatat gagtgaggat
     1981 gtgtaatttt ctcctatctg aactgtgctt aatatctgcg accagccacg ttccaggtaa
     2041 agagtattac tttgccatgt gcctttcata tcatcgggtt tgctatagct ggcatcagag
     2101 tgtaactgcc aactaaataa atttaatcca ctgttaaatc ggccataact ggctgtatga
     2161 ctgttattat tgctgtcata agagcgatac tgacttaaat tataggaggt ataaaaagca
     2221 tcaatgccgc gatcccaact ttccggggga acgtaacctc tttcaagttc attgatatac
     2281 gcctgtggta ctgttaaatt tagtcggtgt tcactgatat cccactgata ttgtccacca
     2341 tgaaccgctt tatccagtaa aatacaatta tcttctgtat tgaggttatc ggtctttacc
     2401 cccagtgtgg ttagcagcac cttaggcaga catggtttgg ctggtgactc tgggatcgtg
     2461 atatcttttt tcccacgcca ctggtgattt acataaaaat caagttcgta gtttcctgga
     2521 agcggttgtt tattatcgaa acgatattcg gaaacttttt ctcctctcat gccgccaatc
     2581 ataaagtgcg tatcaaaagt ttcttcagca taacccggaa aagaatacaa aaaaataata
     2641 gcagcgaccc atcgtgtcgt ccttaacatg actttttcct gttatcttgc ggcgatgtta
     2701 ctatggatgt tattcccgta atcatcaact accgttagtt catattctga tgcgtggtta
     2761 ttttttaatg taatgtcgtt gttcgaaaat ggaggtagta agatactttc attattaact
     2821 ttcacgtttt gtgctttgat agttgtaacc gtt
//
LOCUS       DAPGOF010000298         2799 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.304, whole genome shotgun sequence.
ACCESSION   DAPGOF010000298 DAPGOF010000000
VERSION     DAPGOF010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.304"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            169..1335
                     /gene="nhaA"
                     /locus_tag="RHV13_004634"
     CDS             169..1335
                     /gene="nhaA"
                     /locus_tag="RHV13_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HDV1244067.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1401..2300
                     /gene="nhaR"
                     /locus_tag="RHV13_004635"
     CDS             1401..2300
                     /gene="nhaR"
                     /locus_tag="RHV13_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HDV1244068.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2338..>2799)
                     /locus_tag="RHV13_004636"
     CDS             complement(2338..>2799)
                     /locus_tag="RHV13_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HDV1244069.1"
                     /translation="NFNLNTILQVNLMTCGFEDQSIDTGRFVLSRINNENYQFHHIRF
                     DCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKNELENSEDGAGQIGFQLSNDGTN
                     EIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
BASE COUNT          662 a          612 c          709 g          816 t
ORIGIN      
        1 acccgtcagc gcttacagcc gtcaaaaacg catctcaccg ctgatggcgc aaattcttct
       61 atagatcgta aaaaacgaat tattcctaca ctataatctg attttaacga tgattcgtgc
      121 ggggtaaaat agtaaaaacg atctattcac ctgaaagaga aataaaaagt gaaacatctg
      181 catcgattct ttagcagtga tgcctcggga ggcattattc ttatcattgc cgctatcctg
      241 gctatgatta tggccaacag cggcgcaacc agtggatggt atcacgactt tctggagacg
      301 ccggttcagc tccgggttgg ttcactagaa atcaataaaa acatgctgtt atggataaat
      361 gacgcgttga tggcggtatt tttcctgtta gtcggtctgg aagttaaacg tgaactgatg
      421 caaggatcgc tagccagctt acgccaggcc gcatttccgg ttatcgccgc tattggtggg
      481 atgattgttc cggcattact ctatctggcc tttaactatg ccgatccgat tacccgcgaa
      541 gggtgggcga tcccggcggc tactgacatt gcctttgcac ttggtgtgct ggcgttgttg
      601 ggaagtcgtg ttccgttagc gctgaagatc tttttgatgg ctctggctat tatcgacgat
      661 cttggggcca tcattatcat cgcattgttc tacactaatg acttatcgat ggcctctctt
      721 ggcgtcgcgg ctgtagcaat tgcggtactc gcggtattga atctgtgtgg tgtacgccgc
      781 acgggcgtct atattctggt tggcgtagtg ttgtggactg cagtgttgaa atcgggggtt
      841 cacgcaaccc tggcgggggt aattgtcggc ttctttattc ctttgaaaga gaagcatggg
      901 cgttctccag cgaagcgact ggagcatgtg ttgcacccgt gggtggcgta tctgatttta
      961 ccactgtttg catttgctaa tgctggcgtt tcactgcaag gcgtcacgct ggatggcttg
     1021 acctccattc tgccattggg gatcatcgct ggcttgctga ttggcaaacc gctggggatt
     1081 agtctgttct gctggttggc gctgcgtttg aaactggcgc atctgcctga gggaacgact
     1141 tatcagcaaa ttatggctgt ggggatcctg tgcggtatcg gttttactat gtctatcttt
     1201 attgccagcc tggcctttgg tagcgtagat ccagagctga ttaactgggc gaaactcggt
     1261 atcctggtcg gttctatctc ttcggcggta attggataca gctggttacg cgttcgtttg
     1321 cgtccatcag tttgacagga cggtttaccg gggagccata aacggctccc ttttcattgt
     1381 tatcagggag agaaatgagc atgtctcata tcaattacaa ccacttgtat tacttctggc
     1441 atgtttataa agaaggttct gtggttggcg cagcggaggc gctttattta acaccacaaa
     1501 ccattaccgg acagattcgg gcgctggaag agcgcctgca aggcaaatta tttaagcgca
     1561 agggacgtgg tctcgagccc agcgagctgg gggagctggt ctatcgctat gccgataaaa
     1621 tgttcacctt gagccaggaa atgctggata tcgtgaacta tcgcaaagaa tccaatttat
     1681 tgtttgacgt tggcgtggct gatgcacttt ccaaacgtct ggtcagtagc gtgctgaatg
     1741 ctgcagtagt agaaggcgag ccgattcatc ttcgctgctt cgaatccacc cacgaaatgc
     1801 tgctggagca attaagccag cataaactgg atatgatcat ttccgactgt ccgatagatt
     1861 ctacgcagca ggaaggcctg ttctccgtga gaattggcga atgtggcgtg agtttctggt
     1921 gtacaaatcc accgccggaa aaaccgttcc cggcttgtct ggaagaacgg cgacttttga
     1981 ttcctgggcg acgttcaatg ttagggcgca aattgcttaa ctggtttaac tcccagggat
     2041 taaacgtaga aatcctcggc gagtttgatg atgccgcgtt gatgaaagct tttggtgcga
     2101 tgcacaatgc aatcttcgtt gccccaacgc tttatgcata tgacttttat gccgataaaa
     2161 ctgtcgtaga aattggtcgc gtcgagaatg tgatggaaga gtaccatgct atttttgctg
     2221 agcggatgat tcagcacccg gcggtacagc gaatctgcaa tacggattat tctgcgcttt
     2281 ttagtccagc ggcgcgttaa tcggcacctc ccccaaagtt aaggtggggg agatagatta
     2341 gttgtacata accacgattt tgactcggct cattatttgc ccgcttgaga cattatttcc
     2401 atatgtacgc gggcgaataa atagaggaat ctgattactt ccttcatggg gatgctgaaa
     2461 agagtagtaa ttgcttttac catactggat ttcattcgta ccatcattgg agagttgaaa
     2521 gcctatttga cccgcaccgt cctcactgtt ttctaactca ttttttaata tgctggttcc
     2581 tgactcctgt atcgtcactg gttcaaagta atattcaacg tcactgggtt caaaaactaa
     2641 ttcaccttgc tcgccctgga tgcagtcaaa tcttatatga tgaaactgat aattttcatt
     2701 attgatacga gacagtacaa atcgtccagt gtcgatggat tgatcttcaa aaccacaggt
     2761 cattaaattt acctgtaaaa tagtatttaa attaaaatt
//
LOCUS       DAPGOF010000299         2793 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.305, whole genome shotgun sequence.
ACCESSION   DAPGOF010000299 DAPGOF010000000
VERSION     DAPGOF010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.305"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..341)
                     /locus_tag="RHV13_004637"
     CDS             complement(<1..341)
                     /locus_tag="RHV13_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1244070.1"
                     /translation="MKTLDVISTQLNFNNLLNQAGYNLSEIVVKRYSAAPAFNEGHWL
                     FIAEQYNRYIEVVSLLSKASESGEQHVDIFINMFEDPIDDIISRNIEPKTFATLYKYI
                     ERIPFTPDVKK"
     gene            complement(749..1930)
                     /locus_tag="RHV13_004638"
     CDS             complement(749..1930)
                     /locus_tag="RHV13_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDV1244071.1"
                     /translation="MKLFSISSRDTSMDAMFYIQASTKQQASYIFSTLSPKPTANCQL
                     TDLQNETVVPSISPISLSLNPWSINSQSRTRELSMATHGLNLICAVTDEHTRQIVDFC
                     DNLGQQLKDPDDKSAWVKLINSECALLNSPRLYPVLLEPETPAQFEIFVPAPEDGDHD
                     TVIASVVEECFGDECRYRATDKAFAMSEKPIIRLEAHFNDKREKVLELTFSSFNLSLS
                     ASVYEHQLRQILLLCEDFGVRLQGRAGASCHINMRSAYRRLAHVGTGSRPGDGSDVRG
                     YTSSKEQKDVKGLLDKSTRGSELVPARHGQQFNGVLVNATPAPATTGFIYLSHDFPEG
                     QFMMTAEVLHVGITPGGIYYIETLDGYRFIVTAFEKGLMNGLREFLAVNEDYFKYYRW
                     S"
     gene            complement(1939..2235)
                     /locus_tag="RHV13_004639"
     CDS             complement(1939..2235)
                     /locus_tag="RHV13_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001439488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDV1244072.1"
                     /translation="MIHSFKDRRLEKFFRNGKTTAGIPSEIINAILCRLETLDNVQSE
                     RELLSNSLRYERLRMTSNRYSSIRVNSKYRLFFEWNDGAHNVHLSAHDYKSLIH"
     gene            complement(2285..2755)
                     /locus_tag="RHV13_004640"
     CDS             complement(2285..2755)
                     /locus_tag="RHV13_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1244073.1"
                     /translation="MTKYYDRSGIEISSAKIRCVDSVKGTAEYTFRILCDKCNGRGER
                     KHFFRSRCMACKATGYSLETTRTAYTLNALYRINAQAARKVSASLQNERLRTENAHNS
                     AFNAWCRSHQKMVDAITQQSSSNNFLESLKSSLTHQRQLSDKQLAVAARILGIH"
BASE COUNT          759 a          621 c          611 g          802 t
ORIGIN      
        1 ctttttttta cgtccggtgt aaaaggtata cgttcaatgt atttataaag tgtcgcaaat
       61 gttttgggtt caatgtttcg ggagataatg tcatcaatcg gatcttcgaa catattgata
      121 aagatatcga catgctgctc tccgctttct gatgccttag aaagaagact gaccacttca
      181 atgtagcgat tatactgctc tgcaatgaac aaccagtgcc cttcgttaaa ggcgggtgca
      241 gcgctgtagc gtttaacaac aatttctgaa agattatatc ctgcctgatt cagcagatta
      301 ttaaagttga gttgagttga aatcacgtct aacgttttca taaaacctcc ttttcatctg
      361 tacttattat actgttaccc aacccccagc taataacctc ccagcaaatt ttcagaaaaa
      421 aaacaccgcc ttttatttaa gattgaacac atccttttta aataccaaca atgattatga
      481 aaccacaatg taagtgaagc gccattatgg aaaagtaact tttgtgcagc tcataaattt
      541 tatttattag ttacgttcct ttgtcttgtg gtcgaagttc atcaggcgag gcctaagaag
      601 aatatgtgtg gtttcagcca atgcggtgat cgggcattat aatttggtcg ggctattata
      661 cgtgggtaaa ggtagtggta gggagaggat caggttaaat tgcattttcc cggttgcctt
      721 ccctggccta ccggtttcag gtcagacact aagaccagcg gtaatactta aagtagtcct
      781 catttacagc caaaaactcc cttaaaccgt tcattaatcc cttttcaaac gctgtaacga
      841 taaaccggta tccgtccagt gtttcaatgt agtagatccc gcccggtgtt attccaacat
      901 gcaacacctc ggctgtcatc ataaactggc cttccggaaa atcatgcgac aggtatataa
      961 aacccgtcgt ggccggtgcg ggtgtggcat tcactaagac accgttaaac tgctgcccgt
     1021 gcctcgccgg tacaagctca cttcccctgg ttgacttatc gagcagcccc ttaacatctt
     1081 tttgctcttt agatgaggta tagccccgca catcactccc atcacctggg cgagagccgg
     1141 tgccgacatg tgcaagacga cggtaagcgc tgcgcatatt gatgtggcag gatgccccgg
     1201 ctctaccctg aagccttacg ccaaaatctt cgcaaagtaa taaaatctga cgtagctggt
     1261 gttcatacac ggacgcagaa agtgaaaggt tgaaactgga aaaggtcagt tctaaaacct
     1321 tttcccgttt gtcattgaaa tgtgcttcca gcctgatgat tggcttttcc gacattgcaa
     1381 aagctttgtc cgttgctctg taccggcatt catctccgaa acattcttca acaacgctgg
     1441 ctataaccgt gtcgtgatcg ccatcttccg gagctggcac aaagatttca aattgtgccg
     1501 gtgtctccgg ctccagcaaa acgggatata aacgcggaga attaagcaat gcgcattcag
     1561 aattaatgag cttaacccag gcgcttttgt cgtccggatc cttaagctgc tgtccaaggt
     1621 tatcgcaaaa gtctactatc tgacgggtat gctcatccgt gactgcacag atcaggttaa
     1681 gaccatgcgt ggccatactc aattcccggg ttcgggactg ggaattgatc gaccaggggt
     1741 taagtgaaag cgaaataggg cttatggaag ggacgaccgt ctcattctgc aaatctgtaa
     1801 gctggcagtt cgcagtaggc ttcggggaaa gggtactaaa tatgtaactt gcctgttgtt
     1861 tagtactggc ctggatataa aacatcgcgt ccatgcttgt atcccgggat gaaattgaaa
     1921 atagcttcat ttttaacctt aatggatgag cgatttatag tcatgtgctg acaaatggac
     1981 attatgtgcg ccatcattcc attcaaagaa taatcgatac ttagagttta ctcgaatgga
     2041 ggaataacgg tttgacgtca ttcttaagcg ttcataacgc aagctgttgg ataataactc
     2101 ccgctcactc tggacattat ccagcgtttc gagtcgacac agaatcgcgt taataatttc
     2161 agaaggaata cctgccgttg tttttccatt acggaagaac ttttcaagcc gtctgtcttt
     2221 aaatgaatgg atcatagttc ctccgtaata attgacctta aaattaaatt aaaagggctt
     2281 tgaattaatg aatccctaaa atgcgggcgg cgactgccag ctgcttatca cttaactgac
     2341 gctggtgggt taaagaagac ttaaggcttt ccagaaaatt attactgctg gattgctggg
     2401 tgattgcatc aaccattttt tgatgagatc tgcaccatgc attgaacgca gagttgtggg
     2461 cgttttcagt cctcaaacgc tcgttttgca aggatgctga cactttgcgg gcagcttgag
     2521 cattaatgcg gtacagcgca ttcagcgtgt aagcagtgcg ggtcgtttcg aggctgtaac
     2581 ccgtggcttt acaagccata cagcgacttc taaaaaaatg cttacgctct ccccgaccat
     2641 tgcatttatc gcaaagaata cgaaaagtat attccgcagt accttttaca gaatcaacac
     2701 agcggatttt tgcgctggag atttcaattc cactgcgatc ataatatttg gtcataatgt
     2761 gattcctccg gttagttctg gagtaatcat gac
//
LOCUS       DAPGOF010000300         2791 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.306, whole genome shotgun sequence.
ACCESSION   DAPGOF010000300 DAPGOF010000000
VERSION     DAPGOF010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.306"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            146..1540
                     /locus_tag="RHV13_004641"
     CDS             146..1540
                     /locus_tag="RHV13_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HDV1244074.1"
                     /translation="MKKSTLSLAIGLLLACSTGMAKTQHLTLEQRMALLEERLEAAEM
                     RAAKAESQVKQLQTQQAAEIREIKAAQGNTPVNGQATAESAKKNATSPNLLLSGYGDL
                     KIYGDVEFNMDAESNHGLLAMTNADVNSDPTNEQWNLNGRILLGFDGMRKLDNGYFAG
                     FSAQPLGDMHGSVNIDDAVFFFGKENDWKVKVGRFEAYDMFPLNQDTFVEHSGNTAND
                     LYDDGSGYIYMMKEGRGRSNAGGNFLVSKQLDNWYFELNTLLEDGTSLYNDGNYHGRD
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVNNAYGYTDSKGNFVDQSDRTGYGMS
                     MTWNGLKTDPDNGIVVNLNTAYLDANNEKDFTAGINALWKRFELGYIYAHNKIDEFSG
                     VVCDNDCWIDDEGTYTIHTIHASYQFANVMDMENFNIYLGTYYSILDSDGDKKHGDDT
                     DDRYGARVRFKYFF"
     gene            complement(1581..1895)
                     /gene="rhaM"
                     /locus_tag="RHV13_004642"
     CDS             complement(1581..1895)
                     /gene="rhaM"
                     /locus_tag="RHV13_004642"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HDV1244075.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTEICQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            complement(1905..2729)
                     /gene="rhaD"
                     /locus_tag="RHV13_004643"
     CDS             complement(1905..2729)
                     /gene="rhaD"
                     /locus_tag="RHV13_004643"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HDV1244076.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAL
                     YHDNFHPQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELRALGQRFGVTPLASALAL"
BASE COUNT          701 a          633 c          737 g          720 t
ORIGIN      
        1 gatttactca tattatgcgg cgggatacta tccatcggcc tgctgaagtt ggattaaaaa
       61 ttcgaaagtg cacactttat atttctctat atacgtattc cttttggaaa gcgtagggcc
      121 tcctttattt cagggaacgt aaataatgaa aaaatcgaca ttatctttag ccatcggttt
      181 attattggca tgtagtaccg gtatggcaaa aacacagcat ttaacgctgg aacaacgcat
      241 ggcattgctg gaagaacgcc tggaagcggc agaaatgcgg gcggcaaaag cagagagcca
      301 ggttaaacag ctgcagacac aacaagccgc tgagatccgc gaaattaagg ccgctcaggg
      361 caatacgccg gtaaatggac aggcaacggc ggaatctgca aagaaaaatg ccacctcacc
      421 taatcttttg ctttcaggtt atggcgattt aaaaatctac ggcgacgtag aatttaatat
      481 ggatgcggaa agtaatcatg gcctgctggc aatgaccaac gctgatgtga atagcgatcc
      541 cactaatgaa cagtggaatc tcaatggtcg tattctgtta ggttttgatg gtatgcgaaa
      601 actggataat ggctatttcg ccgggttctc cgcacaaccg ctgggggata tgcacggttc
      661 agtaaatatc gatgatgcgg tattcttctt tggcaaagaa aacgactgga aggtcaaagt
      721 aggccgtttt gaagcctacg atatgttccc gctgaatcag gatacctttg ttgaacattc
      781 cggtaatact gcgaacgatc tttatgacga tggcagcggt tatatctata tgatgaaaga
      841 gggccgcggg cgctctaacg ctggcggtaa tttcctcgtc agcaaacaac tcgataactg
      901 gtattttgaa ttaaacacgt tacttgaaga cggaacatct ttatataatg acggtaatta
      961 tcatggacgc gatatggagc agcagaaaaa tgttgcttat ctgcgcccag taattgcctg
     1021 gtcgccgacg gaagaattca ccgtttccgc agcgatggaa gcgaacgtgg taaataatgc
     1081 ttatggttat accgatagca agggtaattt tgtcgatcag tccgatcgta ccggctatgg
     1141 tatgagcatg acctggaacg gcctgaaaac ggatccggac aatggcattg tggttaatct
     1201 taataccgcc tatttagatg ctaataatga gaaagatttc actgccggga ttaacgcgct
     1261 gtggaaacgt ttcgagctcg gttatatcta cgcgcacaat aagattgatg aattcagcgg
     1321 tgtagtttgt gataacgact gctggattga tgatgaaggg acgtacacca ttcacaccat
     1381 tcatgcgtct tatcagttcg ctaatgtgat ggatatggag aactttaata tttacctcgg
     1441 gacgtattac tccattctgg atagcgacgg cgataaaaaa cacggtgatg atactgatga
     1501 ccgttacggc gcacgcgttc gctttaaata cttcttctga tgaaggcgcg gtgctggata
     1561 ttttccggca ccgcaactca ttaaggcagg taaaacactt cttgcagctc gctactcacc
     1621 gggctgttat ccgggttagc gggcataaca tcggtcatat atttccacca acgttggcag
     1681 atttcagtgc tggctaccgc attccaccgt tcttcagatt caatctctac cgtggcaaac
     1741 agcagattac gcgctttgtc gaggtaaatg gcgtagttat gcgcaccgtg agatttcaac
     1801 actgcttcca gttccggcca gatgggatta tgccgacgct gatactcttc gtgggcgtcg
     1861 gggtttacct gcatgacaaa ggctttgcgg atcatgtttg ctccttacag cgccagcgca
     1921 ctggcgagtg gtgtaacgcc gaaacgctgg ccgagcgctc tcaactcttc acggctgata
     1981 gtctgtttca tgccgcccat cgaatacacc ttcactaata cttgtgctga tttttctgcg
     2041 gtgtcgatta aaccgaaggt ttcatccagc gtcggtccgc tgccgaagac gccgtggaag
     2101 ggccacaaca ccagcgaatg tttttgcatc tcttgtgcgg tcgcctggcc gatttcgtcc
     2161 gtgccgggca ccatccacgg cagaatgcca acgccatccg ggaataccac cagacattcg
     2221 gtgctgcctt cccacagttg gcgagtaaag accgcggtgt cgttttccag tacataggtg
     2281 agggcgatca ggttggtggc atggcagtgc atgatcaccc gatctttacc gttggtggct
     2341 ttaatacgct cgcagtggga gagaaagtga gccggaagtt cagacgttgg aacggcttcg
     2401 ttggttaacc cccagagaat gtggtaaccc gcgccgtcgc tgtcgacttt tacgacgcct
     2461 aagttagctg caggatcgag ctggacgtta cggaagaatt tgccggagcc ggtgacaata
     2521 aacggcgtat ttgccagtaa aggcatgggt tggctgagcg gaatatagcg cggttgtggg
     2581 tggaagttgt cgtgataaag cgcgatatcg gcgtcatcca ggcgcagcgt caggttgccg
     2641 ccgttgcgct cgtcccagcc tttcagccag gcgtcggtgg tggctttgat cattccctgg
     2701 acaaaccagg actgggtaat gttttgcatg ttctgtgttc ctgtaaattc ggtgttgtca
     2761 gatgcacgac ccgtaggccg gataaggcgc t
//
LOCUS       DAPGOF010000301         2652 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.307, whole genome shotgun sequence.
ACCESSION   DAPGOF010000301 DAPGOF010000000
VERSION     DAPGOF010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.307"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            90..578
                     /gene="eco"
                     /locus_tag="RHV13_004644"
     CDS             90..578
                     /gene="eco"
                     /locus_tag="RHV13_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HDV1244077.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKINNAE
                     VR"
     gene            complement(954..2600)
                     /gene="mqo"
                     /locus_tag="RHV13_004645"
     CDS             complement(954..2600)
                     /gene="mqo"
                     /locus_tag="RHV13_004645"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HDV1244078.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVNDQQGTIA
                     ALLGASPGASTAAPIMLNLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
BASE COUNT          618 a          743 c          669 g          622 t
ORIGIN      
        1 tcataaagca aattaataaa tctgatgaat atgttaacct tcagcgacat catcggtgca
       61 aacctataaa tgaagaagga aagcaaaaaa tgaagaccat tctacctgca gtattgtttg
      121 ccgcttttgc caccacttcc gcctgggcgg cagagagcgt ccagccactg gaaaaaatcg
      181 cgccttatcc acaagctgaa aaaggcatga agcgtcaggt gattcagtta accccgcaag
      241 aagatgaatc taccctgaaa gtagaactgt taatcggtca gacgctggaa gtcgattgca
      301 atttgcatcg tctcggcggg aagctggaaa gcaaaacgct ggaaggctgg ggctatgatt
      361 attatgtctt tgataaagtt agctcgccgg tttccacgat gatggcctgc ccggatggca
      421 agaaagagaa gaaatttgtc accgcgtatc tgggcgatgc tggaatgctg cgttacaaca
      481 gcaagctgcc gatcgtggtg tatacgccag acaatgtaga tgtgaaatac cgcgtctgga
      541 aggcagaaga gaaaattaac aacgcggaag ttcgctaaac tgccgtgaag tgcggcaccc
      601 cataggttgg ataaggcggt tacgccgcat ccaacatcta acgcccgagc cggttgcctg
      661 atgcgacgct ggcgcgtctt atcaggccta caccgctgtg aagtgctcca ccccgtaggt
      721 cggataaggc ggttacgccg catccgacat ccaacgtccg agccggttgc ctgatgcgac
      781 gctgacgcgt cttatcaggc ctacaccgct gtgaagtgct ccaccccgta ggtcggataa
      841 ggcggttacg ccgcatccga catccaacgc ccgagccggt tgcctgatgc gacgctggcg
      901 cgtcttatca gcatacgcca catccggcat accatgccgg atgtggcaca tcattacaac
      961 gcaatatccg ccacttcttt ttgaacgggt tgcggtttca gctgcggttt cggcgtacta
     1021 tctgctgctt gcggtttgtc gtatttcagc cccagcactt cgctggtgta ctgcaactcg
     1081 cgctctgttg cggctacatc accgttcagc ttgcgtccat aagacggaac gatcgctttc
     1141 aacgtagcct gccattgcgg gctggaaacg cgatcgccaa atactttttc cagcagattc
     1201 aacataatcg gcgcggcggt tgacgcccct ggcgatgccc ccaggagcgc ggcaatggtt
     1261 ccttgctggt cattgaccac ttcagtaccc agacgcagta caccaccttt atcggcatca
     1321 cgcttgataa tctgcacacg ctgccccgct tgccacaaac gccagtcctc ttttttcgct
     1381 tgcggatagt actctttcaa cgcttcaaaa cgatcctctt cactcaacat cacctgactc
     1441 accagatatt tcaccagatc gaaattatcc agcccgacgt gcatcatcgg catcacgtta
     1501 gaggtggtgg tggaactcat cagatcccac aatgaaccgt ttttgaggaa tttggttgag
     1561 aaggtggcaa atggcccaaa cagcactacg cgtttaccgt ccagaacgcg ggtatcgata
     1621 tgcggaaccg acatcggtgg tgcgccaacg gatgctttac cgtaaacctt cgccagatgg
     1681 tgattaacca cgtccgggtt ttccgaaaca aggaactgac cgcccaccgg gaaaccggcg
     1741 tagtctttcg cttccggaat ccccgattcc tgtaacagct tcagtgccgc accgcccgcg
     1801 ccgataaaga caaatttcgc acggatgttc tgtgcagtgc catttttcag atcggcaacg
     1861 gtaacggtcc aggtgttatc gtcattacgc tttagggcgc ggacttcgct gctgagttgc
     1921 agcgagaagt tagatttctt ctgcaaggaa gcaattaact ggcgggtgat ctcgccgtag
     1981 ttcacatcgg taccaatttc cgtacgcgtg gctgccactt tctgttgcgg atcgcgccct
     2041 tccatcacta acggtgccca ctctttgatc tgcgcgtgat cttcagagta acgcataccg
     2101 cgaaacagcg agctttgttg caacgcggcg taacgggcgc gcaggaaatt aacgttatcc
     2161 tcgccccaga caaagctcat atgcggaacg gtattgataa atgaacgcgg agtacgcagc
     2221 acgccgcgct cgacctggtg cgcccagaac tggcgggaaa tctgaaatgc ttcgttaatg
     2281 gcgactgctt tttcaatact gatgctgcca tcggcgtttt gcggggtgta gttcagttcc
     2341 atcagggcag aatgcccggt tccggcgtta ttccagccgt tcgaactctc ctgcgcgaca
     2401 ccctccaggc gctccaccat ggtcatcgac cattcaggct ccagctcgcg taaataggtc
     2461 cccaacgtgg cgctcataat gccgccgcca atcaacagta catcagtttc ctgctcctcg
     2521 gacgctttcg cttttgccgc catcgaaacg gcattaagcc ccacggccat cgagaagagc
     2581 atggcagtca cttttttcat cttgttaatg ccttactttt agtcgcttta ttgcaggtga
     2641 gatttgcgcg gc
//
LOCUS       DAPGOF010000302         2646 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.308, whole genome shotgun sequence.
ACCESSION   DAPGOF010000302 DAPGOF010000000
VERSION     DAPGOF010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.308"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            68..676
                     /locus_tag="RHV13_004646"
     CDS             68..676
                     /locus_tag="RHV13_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1244079.1"
                     /translation="MEESLEIIKELVLRRKLFFKDDNGNITVNPLLEAETRWYMSKSF
                     EYTCLSHGLDACEFRAELKSWLYYHSHRSISENTKLAECRNDDEIILHDCNDDMGWDI
                     FFDQDYLMSEKKLAVKWTDREIMDVYIKAFKSTLELFDELVSCDLLTKRNAFGKLEIN
                     PIFENHYEWIMSEAFEIVGNHLGYNVPQIRKLMATICQMNLK"
     gene            734..1792
                     /locus_tag="RHV13_004647"
     CDS             734..1792
                     /locus_tag="RHV13_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDV1244080.1"
                     /translation="MSNAAMKLNETSSDAYEKLEALLSPDVIKLKHYVDKGEYLLVLA
                     KDLFGIPEMDPKMAVPVFKTKTSYRAPLNKDYIPNPRILEQVVKLLISPDIDLSVCLK
                     GESGSGKTEMVMYISHMMNWPLTIKQINSNIRVDELEGERSLNGGNTGFVHSDLVTGF
                     RNGHLILLDEVDKIDPDTAAKLHMPIERKPWSLSANGGEVITANGYTRFIGTANTNMS
                     GGARRFVSSQRQDAAFIKRFLIVEMEKPDKVALTNVLTKRYSSLPFQVIEKFVRVAIA
                     VNDSGTEDSVMDIRQLVAWVGTSMTLKTFSLLDTFKIAFASALPLHVVDLVLQAIDLS
                     LGEDKDKSLEYFTAKKSS"
     gene            1924..2097
                     /locus_tag="RHV13_004648"
     CDS             1924..2097
                     /locus_tag="RHV13_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1244081.1"
                     /translation="MQLNNDYSLLFYHDGKINMEGLIKQMELKGYMYQGAMGNDDVVA
                     EFPGILLISPEKL"
     gene            2161..>2646
                     /locus_tag="RHV13_004649"
     CDS             2161..>2646
                     /locus_tag="RHV13_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3150 domain-containing protein"
                     /protein_id="HDV1244082.1"
                     /translation="MTLNTSMLNKVSAKQISENFAKSYPDLLEGAVITKIEISGCQGS
                     RRTDKIDLSYDGDDITDQKSVSKSEVRWIDIKALSFKSTITSRISTLCSRLCIRYSNM
                     WILPVSSMEEFLEGAQEIEREFQAGIQNVVDNYEMHIEAEKNRSPRMSSLIDQLKLTK
                     DD"
BASE COUNT          826 a          439 c          571 g          810 t
ORIGIN      
        1 cccagatttg ggtggtctgg taatagaatg gacccctgag gatgtgtatg ttgtttttct
       61 atccgctatg gaagagtctt tggaaatcat taaagaacta gtgttgagga gaaagttatt
      121 cttcaaagat gacaatggta atatcacagt gaatccactt ttagaagctg aaacacggtg
      181 gtatatgtca aaatcatttg aatacacttg tttatctcat ggacttgatg catgtgaatt
      241 tagagcggaa ttaaaatcat ggctctatta tcattcgcat cgttcgatta gtgaaaacac
      301 gaaattagct gaatgcagga atgatgatga aatcattttg catgattgca atgacgatat
      361 gggctgggac atcttctttg atcaggatta tttaatgtca gaaaaaaaac tggctgttaa
      421 atggactgac agggaaatta tggatgttta cataaaagct tttaaatcca cattagagtt
      481 gtttgacgag cttgtttcat gtgatctgtt aactaaacga aacgcttttg gcaagttaga
      541 aatcaatcca atattcgaaa atcattatga atggatcatg tctgaagctt ttgaaatagt
      601 gggaaatcat cttggttata atgtgcctca aatcaggaaa ctgatggcaa ctatttgcca
      661 aatgaatctc aaataaaatg ccactggcaa acatctgcca gagggcggtg tttgcatttt
      721 accggagtta aaaatgtcta atgccgctat gaagttaaat gaaacatctt ctgatgctta
      781 tgaaaaatta gaagctcttc tttccccgga tgttataaag ttaaaacatt atgtggataa
      841 aggtgagtat cttttagttc tagcaaagga cctttttggt attccagaaa tggatccaaa
      901 gatggctgtg cctgttttca aaactaaaac ctcgtatcga gctccgctga acaaagacta
      961 cataccaaat ccccgaatac ttgaacaagt ggttaaactg cttatcagtc cggatataga
     1021 tttaagtgtg tgtcttaaag gtgagtccgg ttctggcaag actgaaatgg taatgtacat
     1081 tagccacatg atgaattggc cgctgacaat taagcagatc aacagcaata ttcgagttga
     1141 tgagcttgaa ggtgagcgca gtcttaatgg tggtaataca ggttttgtac acagcgattt
     1201 ggtaacggga tttcgtaatg gtcacctcat tcttctggat gaagtggaca aaatcgatcc
     1261 tgatacggca gcaaaacttc acatgcccat tgaacgtaag ccctggtcac tcagtgctaa
     1321 tggtggtgag gttataactg ctaatggcta cacgcgattt attggtactg caaacacaaa
     1381 catgagtgga ggcgctcgcc ggttcgtttc ttctcaacgt caagatgcag cttttataaa
     1441 gcggttcttg atagttgaaa tggagaagcc cgacaaagtt gctttaacca atgtgcttac
     1501 taaacgatat agctctttgc cttttcaggt cattgagaag ttcgtaagag tagcgattgc
     1561 agtaaatgac tctggtacag aagacagtgt gatggatatt cgtcaattag tagcttgggt
     1621 tggcacgtcg atgacgttaa aaacctttag tcttcttgac actttcaaaa tagcttttgc
     1681 ttctgcttta cctttacatg tggtcgatct tgttcttcaa gctatagatc tttctcttgg
     1741 cgaagacaag gataaatcgc tggagtattt cacagcaaaa aagtcgagtt agatgggatt
     1801 tgttatgaaa cctctgatat ttgtatctca gagttatccc atcttgaatt tagtgcgttt
     1861 ttaagtacgg atggtgctgc taccattgct atagcaaaaa acggaactaa ttttactgga
     1921 cttatgcaac tgaataacga ctattcttta ttgttttatc atgatggtaa aataaatatg
     1981 gaaggtctga ttaaacaaat ggaattaaaa ggttatatgt atcagggagc aatgggcaat
     2041 gatgatgttg ttgctgagtt tcccggtatt cttttaattt caccagagaa gctttaatat
     2101 caaccctatc gggaaatctt tcccgttggg gagggtttcc cctttttttt ggagaaaccc
     2161 atgactttaa atacttcaat gcttaacaaa gtatctgcaa aacaaatctc tgagaatttt
     2221 gcaaaatcat atccagattt actggaaggt gcagttataa caaaaattga aataagtggc
     2281 tgtcaaggat cgcgtcgaac cgataaaatc gatttgtctt atgatggaga tgatattacg
     2341 gatcaaaaaa gcgtatctaa aagcgaagta cgctggattg acataaaagc ccttagcttc
     2401 aaaagtacga ttaccagcag aatatcaact ctttgctcac gcttgtgcat ccgctatagc
     2461 aatatgtgga ttttaccagt atccagtatg gaagagtttc tggaaggggc ccaagagatt
     2521 gaacgagagt ttcaggcagg tattcaaaac gttgtagaca attatgagat gcatattgag
     2581 gctgaaaaaa atagaagccc aagaatgtct tctttgattg atcagttgaa attaactaaa
     2641 gatgat
//
LOCUS       DAPGOF010000303         2645 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.309, whole genome shotgun sequence.
ACCESSION   DAPGOF010000303 DAPGOF010000000
VERSION     DAPGOF010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.309"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            116..751
                     /locus_tag="RHV13_004650"
     CDS             116..751
                     /locus_tag="RHV13_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1244083.1"
                     /translation="MKSIILSMVAATALLSGCADSKTQPVPESPLTSNDHQITLKIDT
                     VRTDLPDSSPVCLNGMNIYESLSERHPGLFAKNQNVIFRSAFFDDGQAISSETIRKIM
                     QSTPCVESQTTSAGSWEPATELIPRQAISGDTTLAEKDRTAGFTMRIAPEIVRGNFHL
                     LLTTTDPIRKQTVEQNILLKPDQPLIVSGFTGVNADSQSENRIVIITPHVR"
     gene            923..1210
                     /locus_tag="RHV13_004651"
     CDS             923..1210
                     /locus_tag="RHV13_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDV1244084.1"
                     /translation="MLSMIAASAIALSAPVYASDPCASVLCLYGKAIGSGGGSECKSA
                     EKDFFNIIKKKKGSIRWSKTFDARKAFLNQCTTADPAAISKIMSKFGRSWG"
     gene            1213..2448
                     /locus_tag="RHV13_004652"
     CDS             1213..2448
                     /locus_tag="RHV13_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpQ domain-containing protein"
                     /protein_id="HDV1244085.1"
                     /translation="MKKLIIIPTTLLLNACSHAPEPAQAKGGWYELNTTIEQVRADTY
                     YNGVVLQQPVSQPNSTVQYSNDNYLKKEAINVTPAPRIAETVKPRSEQSATAPKNQDS
                     NNSFLKPTTNSITSSGEIKTAAKGATKQHSEQPALPPAKPISSNNHQAQTAQNTDQQT
                     KPRPEQPPLMSKSGFLKKNANNAVPATTLTGPSTPEKQIYFVRHDGKGSLQKAVKEIV
                     PSDWTAEISPEVTKTFRRTISWQGNDQWPYVLDKMLRNYGLTVIKNTEKRHLLIDFQQ
                     QKGKTSQKAPVSPDKTPTSPVRTPVSSDKLPQKPLTPATVPQKQTEKLTWNLKKGSTL
                     RDGLTEWASSSPCGNSTWNLVWDTPVNYRIDAPLIFRGNFESALKDTLMLYLSADKPL
                     YAFRNIAQCVITIKDTQQE"
BASE COUNT          827 a          668 c          544 g          606 t
ORIGIN      
        1 gcacaagaag aaagtaaatg aattaatgga acgtctacaa taacgttctc ctctcccccc
       61 gtcacaaagc ccgcctcgtg cgggttttct tttgcctgaa atatggagga actccatgaa
      121 atccatcatt ttatcgatgg ttgccgccac cgcattactg tcaggctgcg cggatagcaa
      181 aacacaacca gtacctgagt caccgttaac ctcaaatgac caccagataa cgttgaaaat
      241 tgataccgtg cgaacagacc tgccggacag ttctcctgtt tgtttgaatg gtatgaatat
      301 atacgaaagt ctgagcgagc ggcatccagg cttgtttgcc aaaaatcaga acgtgatttt
      361 ccgtagtgca ttctttgatg acggtcaggc gatatcctcc gaaacaatcc gcaaaataat
      421 gcagagtacc ccgtgcgttg aatcacaaac aacatctgcc ggaagctggg aacctgccac
      481 ggaactgatc ccccgtcagg caatatccgg tgacactaca ctggctgaaa aagatcggac
      541 tgctggcttc acaatgcgga tagcaccgga gattgtgcgt ggaaatttcc acctgctact
      601 cacaaccact gacccgatac ggaaacagac tgtcgagcag aatattttac tcaaaccgga
      661 ccagcccctt atcgtgagtg gttttactgg agttaatgct gatagccaaa gtgagaaccg
      721 gattgtcatc attacgccac acgtgcgttg attcccctga tatttaaatt acccgcctcg
      781 tgcgggtttt cttttgtctg aaatatggag gaacaccatg aaatccacca ttttattgat
      841 ggttgccgcc accgcagcat tacgaccagg ctgcgcagat agtagaacac accccgtgcc
      901 tgagacaccg ttaagcaaaa tcatgttatc gatgattgcc gcaagtgcta tcgcactctc
      961 tgcccccgta tatgcgtctg atccgtgtgc ttccgttctt tgtctgtacg gtaaagctat
     1021 cggtagcggc ggcgggagcg aatgcaaaag cgccgaaaag gacttcttta atatcatcaa
     1081 aaagaaaaag ggatctattc gctggagcaa aacctttgat gcccgaaaag cgtttcttaa
     1141 tcaatgcacg accgcagacc ctgcggcgat ctcaaaaatc atgagtaagt tcggacgttc
     1201 ctggggttaa ccatgaaaaa gctcattatt attcccacca cattattgct gaatgcgtgt
     1261 tcccatgccc ctgaacccgc acaggcaaaa ggaggttggt atgaactgaa taccacaatt
     1321 gagcaggtaa gagcagacac atactataac ggggtggtcc ttcagcagcc cgtgtcacag
     1381 ccaaacagca ctgttcaata cagcaatgat aattatctca aaaaggaagc cataaacgta
     1441 actcccgcgc ctcgtattgc agaaaccgta aaaccacgtt ctgagcaatc agcgacagcg
     1501 ccaaaaaacc aggacagcaa taatagtttt ctgaaaccaa caacaaacag cataacttca
     1561 tctggtgaga taaaaacagc agccaaagga gcaactaaac agcattctga acaaccagca
     1621 ctccctcccg caaagccaat cagtagcaac aaccatcagg cacaaacggc acaaaatact
     1681 gatcagcaga caaaaccgcg tcctgaacaa ccaccgctaa tgtcaaaaag cggttttctg
     1741 aaaaaaaacg ctaacaacgc tgttccggcc accacgctta caggcccatc cacacctgaa
     1801 aagcaaattt actttgttcg ccatgatggt aaaggctcgc tgcaaaaagc agtaaaagaa
     1861 attgttccat cggactggac ggctgaaata tccccggagg taacgaaaac tttccgtcgc
     1921 acgatatcct ggcagggcaa cgatcaatgg ccttatgtcc tggataaaat gcttcgcaac
     1981 tacggtctga cggtaattaa gaacaccgaa aaaagacatc ttctgattga ttttcaacaa
     2041 cagaaaggga aaacatcaca aaaagcacct gtatctcctg acaaaacccc tacatctcct
     2101 gtcaggacac cagtttcctc tgacaagtta cctcaaaaac cgctaacgcc tgccaccgtt
     2161 ccgcaaaaac agacagaaaa actcacctgg aatcttaaaa aaggttcgac gctgcgggat
     2221 ggcctcacag aatgggcgtc aagttcgccg tgtggtaatt caacctggaa tcttgtatgg
     2281 gatactcctg tgaactacag aatagatgct cctcttattt tccggggaaa ctttgaatct
     2341 gcattaaaag atacgctgat gctttatctt tcagcagata aaccgttata cgcattcagg
     2401 aatattgcac aatgcgtaat cacaattaaa gacacacaac aggagtaatc gctatgggtt
     2461 ggttcttcag cttgctcctg atattcctgt caataggcag ttactatctg acagataaca
     2521 ataaccaaac catacagcga atgaagttgc ttcctgcaaa acaggaagca attgaatttg
     2581 tccatttcgc aaatgttatc aatgactatc tttataaata tccagacaaa cgtaattcag
     2641 gagga
//
LOCUS       DAPGOF010000304         2592 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.310, whole genome shotgun sequence.
ACCESSION   DAPGOF010000304 DAPGOF010000000
VERSION     DAPGOF010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.310"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..545)
                     /locus_tag="RHV13_004653"
     CDS             complement(<1..545)
                     /locus_tag="RHV13_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ref family protein"
                     /protein_id="HDV1244086.1"
                     /translation="MKTIEQKLEQRREWQKAARERAIARQREKLADPAWRESQYQKMR
                     DSIDRRIAKQKERPPASKTRKSAVKIKSRGLKGRTPTAEERRIANALGALPCIACYMH
                     GVISEEVSLHHISGRTAPGCHKKQLPLCRWHHQHAAPAEVREKYPWLVPVHADGVVGG
                     KKEFTLLNKSEMELLADAYEMA"
     gene            complement(791..1102)
                     /locus_tag="RHV13_004654"
     CDS             complement(791..1102)
                     /locus_tag="RHV13_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysogeny establishment protein"
                     /protein_id="HDV1244087.1"
                     /translation="MRKDFKIDGKYVVLSVSSQIQSPSVIVTVKLSDRMPDIDSISVA
                     FPVKSMRSAEHFVMNATEEEARRGLTRVMAEFGELLGKVNNALSISSARSKALTASMM
                     K"
     gene            complement(1153..2184)
                     /locus_tag="RHV13_004655"
     CDS             complement(1153..2184)
                     /locus_tag="RHV13_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDV1244088.1"
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKM
                     LLSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRS
                     GLPRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRN
                     LAFLGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVT
                     KLVERWISVSGVADDPNNYLFCRVRKNGVAAPSSTSQLSTRALEGIFEATHRLIYGAK
                     DDSGQRYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGA
                     MVRLLEDGD"
     gene            complement(2192..2413)
                     /locus_tag="RHV13_004656"
     CDS             complement(2192..2413)
                     /locus_tag="RHV13_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase"
                     /protein_id="HDV1244089.1"
                     /translation="MGYSAAKVSTHLELEKNRGYWRAKGFDRDSCQLSLSRGEEKIER
                     TRGRWRFYDENHKQVKAEPILYTLLKTII"
BASE COUNT          629 a          669 c          575 g          719 t
ORIGIN      
        1 gccatctcat aggcgtcagc cagtaactcc atctctgact tgttcagcaa ggtgaattct
       61 ttcttgcctc caaccacacc atcggcatga acagggacca gccaggggta tttttctctt
      121 acttcagccg gtgctgcatg ctggtggtgc catctacaaa gtggcaactg ctttttgtga
      181 caacccggcg cggtacgacc ggagatatgg tgcagagaca cctcttcaga tattactcca
      241 tgcatatagc aggcaatgca ggggagagcg ccaagagcat tggctatacg ccgttcctcc
      301 gccgtcggtg ttcgtccctt caagccacga gattttattt ttaccgcact tttccgcgtt
      361 ttgctggctg gtgggcgctc tttctgttta gcgatacggc ggtcgataga atcccgcatt
      421 ttctgatatt gcgattctcg ccaggcgggg tcagccaact tttcccgttg ccgagcgatc
      481 gctcgttctc tggctgcctt ctgccactcg cggcgctgtt caagtttttg ttcgattgtt
      541 ttcatatggc aaaaaaaagg cggcctaatg gccgccaatg atgtcaagga gtgaagtaat
      601 ggcaacgtct tcgtagttga caaaaactgc ggctcaatta tagcaatcaa ttagagcaat
      661 ggtagatatt ttatttatcg cgaatcacat tttttcactt cagtacctgt gtgctatact
      721 ccttcttgat tgattggatg cggaatacaa acccgctctt ttgtgcagcc tggctccttg
      781 ccaggctttt ttatttcatc atggaagctg ttaacgcttt ggatcttgct gaactgattg
      841 aaagggcatt gtttacctta cccaggagtt cgccaaattc cgccatcact ctagtaagcc
      901 cgcgccgcgc ttcctcctcc gttgcattca tcacaaaatg ttcagcactc cgcatgcttt
      961 taacggggaa cgcaacagat atcgagtcga tatcaggcat cctatcgctc aactttacgg
     1021 tgacaatgac agatggtgac tgaatttgag agcttacaga cagcaccaca tattttccgt
     1081 ctattttgaa atccttccgc atgtgtcacc ataaatatca aataattaga gcaatcaagc
     1141 gtaaataaac ggctagtcgc catcttccag cagacgcacc attgcccccg tttcactatc
     1201 caggttacga atgtagttca tgacaatatt tacgttggtc cagccaccag cttgcatgat
     1261 ctccggtatt gaaactccgg cgcgggccat atctcgcgcg gcaccgacac gggcactatg
     1321 tccagaccag gccaggtatc gctgaccaga gtcatctttt gccccgtaaa tcaatcggtg
     1381 agttgcttca aaaatccctt ccaaggcgcg agttgatagc tggctggtgg atgatggcgc
     1441 ggcaacacca ttttttctga cccggcaaaa caggtagtta ttcggatcat cagctacacc
     1501 agagacggaa atccatcgct cgaccagttt agttaccccc aggctaagtg ccttctctac
     1561 acctgcggta ctaaccagcg ttttcgttct gccaatatgg attaacattc tcccaccgtc
     1621 agtacgtgag atatctttaa ccctgatcct ggcaatttcg gctatacgta acagggtgtt
     1681 ataagcaatc cccagaaatg ccagattacg tatatcctgg cagcgatcgc tattttccat
     1741 gagtgaacga acctggtcga aatcagtgcg ttcgaacgct agagcctgtt ttgcacgttc
     1801 accggcatca acgttttctt ttcggatccg ccgcataacc agtgaaacag cattgctgtc
     1861 acttggtcgt ggcagcccgg accgacgatg aagcatgttt agctggccca aatgttgctg
     1921 gatagttttt actgccagac cgcgcgcctg aagatataga agataatcgc gaacatcttc
     1981 aggttctgcg ggaaaccatt tccggttatt caacttgcac catgccgccc acgaccggca
     2041 aacggacaga agcattttcc aggtatgctc agaaaacgcc tggcgatccc tgaacatgtc
     2101 catcaggttc ttgcgaacct catcactcgt tgcatcgacc ggtaatgcag gcaaattttg
     2161 gtgtacggtc agtaaattgg acatttaaca ctcagataat agttttaagt aaagtgtaca
     2221 ggattggctc tgcctttacc tgtttatggt tctcgtcata gaaacgccag cgaccgcgcg
     2281 tgcgttctat tttctcttca ccgcgcgata atgacagttg gcaactatca cgatcaaacc
     2341 cttttgcccg ccagtaacca cggtttttct caagctcaag atgagtggac actttagcag
     2401 ctgaatatcc catttttcac ctctgattga ttggtggtgc taagtgcgct acgcgaaatc
     2461 tggagcacta acactgccaa catttcgcag attttacgta gcgcaacctt tatcaaatga
     2521 tcaagtgatc actatttaac ctgataaggt attgaactgt atggatttac aggtaaattg
     2581 atcatgttca at
//
LOCUS       DAPGOF010000305         2522 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.311, whole genome shotgun sequence.
ACCESSION   DAPGOF010000305 DAPGOF010000000
VERSION     DAPGOF010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.311"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            74..2353
                     /gene="ydeP"
                     /locus_tag="RHV13_004657"
     CDS             74..2353
                     /gene="ydeP"
                     /locus_tag="RHV13_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HDV1244090.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLSWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRHDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQATLPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
BASE COUNT          654 a          603 c          633 g          632 t
ORIGIN      
        1 taatcccttt gtatgtactg tgcatcatcg ctattacaaa tcctaataat tcatttccac
       61 acaggataag tagatgaaga aaaaaattga atcctaccag ggtgctgccg gtggttgggg
      121 tgctgttaaa tccgtagcga atgcagtacg taagcagatg gatatacgcc aggatgttat
      181 tgccatgttt gacatgaata agccagaggg ctttgactgt ccgggttgtg catggccaga
      241 tcctaagcac agtgcgtcat tcgacatttg tgaaaacggc gcaaaagcaa tcgcctggga
      301 agtcacggat aagcaggtaa atgcctcttt ctttgctgag aatacggttc aatcattact
      361 ttcctgggga gatcacgagc ttgaggctgc gggacgtctc actcagcctt tgaaatatga
      421 tgctgtcagc gactgttaca agccattaag ctggcaacaa gctttcgacg aaattggcgc
      481 acgccttcaa agctatagtg atcccaatca ggttgaattc tatacttcgg gccgcacttc
      541 caatgaagct gcctttcttt atcagctttt tgcccgtgaa tacgggagca ataactttcc
      601 cgactgctcc aacatgtgcc atgaaccgac aagcgtgggt ttggcagcga gtatcggtgt
      661 aggtaaaggg accgtgttgc tggaagactt tgagaagtgc gatttagtca tttgcattgg
      721 gcataaccct ggtacaaacc accctcgcat gctgacttcg ttgcgcgctt tagtgaaacg
      781 gggagcgaaa atgatcgcca tcaatcctct acaggaacgt ggcctggagc gatttaccgc
      841 accgcaaaac ccgtttgaaa tgctgacgaa ctctgagact cagttggcca gtgcctacta
      901 caacgtgcgc attggtggcg atatggcgtt gctcaagggg atgatgcgcc tgttaattga
      961 gcgcgatgat gctgcaagtg ccgcaggtcg gccctcattg cttgatgacg aatttattca
     1021 aacgcatacc gtcggctttg acgagctacg ccatgacgtt ctcaattccg agtggaaaga
     1081 tatcgaacgt atttctggac taagtcagac acaaatcgcc gaactggctg acgcatatgc
     1141 cgctgccgaa cgcaccatta tctgttacgg aatggggatc actcagcacg aacatggtac
     1201 ccagaacgta cagcaactgg tcaatttgct gttgatgaaa ggtaacattg gcaagcctgg
     1261 tgcgggtatc tgcccactac gtggtcactc taatgtacag ggcgaccgaa ccgtcggtat
     1321 caccgagaaa ccgtctgcag agtttctggc tcgcctgggt gagcgctatg gcttcacccc
     1381 acctcatgca cctggacatg ctgcaattgc cagcatgcaa gcaatatgta cggggcaggc
     1441 tcgagcattg atctgcatgg ggggcaactt tgcactggca atgccagatc gggaagcgag
     1501 cgctgtaccg ttaacgcaat tagatttggc ggtacacgta gccactaagc ttaaccgctc
     1561 tcatctgctg accgcacggc atagctatat cctgccggtt ctggggcgta gcgagattga
     1621 tatgcaaaaa agcggtgcgc aggcggtaac cgttgaggat tcaatgtcga tgattcatgc
     1681 ctcgcgtggc gtgttaaaac ccgccggtgt aatgctgaaa tcagagtgtg ctgtggtcgc
     1741 gggaatcgcc caggcaacac taccccagag cgtggtagcc tgggagtatc tggtggaaga
     1801 ttatgatcgc attcgcaatg acattgaagc tgtgctgcca gagttcgccg actataacca
     1861 gcgcatccgt catcccggtg gttttcacct gataaatgca gctgctgaaa ggcgctggat
     1921 gacgccgtca ggtaaggcta atttcattac cagcaaaggg ctgttagaag atccctcttc
     1981 agcgtttaac agtaagctgg tcatggcgac agtacgcagc cacgatcagt acaacacgac
     2041 gatttatggt atggatgatc gctatcgagg ggtattcggt caacgagatg tggtctttat
     2101 gagtgctaaa caagctaaaa tttgccgtgt aaaaaacggc gaaagagtta atcttattgc
     2161 gcttacgcca gacggtaagc gcagctcacg ccgcatggat agattaaaag tggtcattta
     2221 ccctatggct gaccgctcac tggtgaccta ttttccagaa tcgaatcaca tgctaactct
     2281 tgataaccac gatccattaa gtggcattcc tggttataaa agtattccgg ttgaattaga
     2341 accatcaaat taattcgcct cctcatttct tctgctgtca tccgcacagc agaagaattt
     2401 ctcattgact attatcgcgc aatttgctca cattgattaa attaaaccac acactataag
     2461 atataaactt ctgcctacag ctgtaagaaa ctccgctcag tactgaagca ccagtcctat
     2521 tt
//
LOCUS       DAPGOF010000306         2463 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.312, whole genome shotgun sequence.
ACCESSION   DAPGOF010000306 DAPGOF010000000
VERSION     DAPGOF010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.312"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(22..975)
                     /locus_tag="RHV13_004658"
     CDS             complement(22..975)
                     /locus_tag="RHV13_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HDV1244091.1"
                     /translation="MVTSIPLLLLILVALIFVKSAVKIVPQGNAWTVERFGKYTHTLS
                     PGLHFLIPFMDRIGQRINMMETVLDIPKQEVISKDNANVTIDAVCFVQVIDAAKAAYE
                     VDNLASAISNLVMTNIRTVVGGMNLDDMLSQRDSINSKLLTVVDYATDPWGIKVTRIE
                     IRDVKPPKELTEAMNAQMKAERTKRARILEAEGIRQSEILKAEGEKQSQILKAEGERQ
                     SAFLQSEARERQAEAEARATKLVSDAIAEGDVQSVNYFIAQKYTEALQAIGTASNSKL
                     VMMPLDSSSLVSSVAGISELLKNVSQDTPKVDMKQAQQYLS"
     gene            complement(1002..1178)
                     /locus_tag="RHV13_004659"
     CDS             complement(1002..1178)
                     /locus_tag="RHV13_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1244092.1"
                     /translation="MADFGSTKQTVTFEEWHELLMDYAELRGGNAADAEAWRADYEAG
                     KTPVEAYCDEWGED"
     gene            complement(1171..1386)
                     /locus_tag="RHV13_004660"
     CDS             complement(1171..1386)
                     /locus_tag="RHV13_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HDV1244093.1"
                     /translation="MPKYQINATIKKPGGGPVKWTHYSDFPMTAEECRKNFSKEKEAG
                     KCFSVDVHISDFICKEIPPQNAGNTYG"
     gene            complement(1379..1885)
                     /locus_tag="RHV13_004661"
     CDS             complement(1379..1885)
                     /locus_tag="RHV13_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiF/GrlA family transcriptional regulator"
                     /protein_id="HDV1244094.1"
                     /translation="MTLYSGDVSISDKQPGENMARKLKINPVIPECVADLKGYPLYII
                     VAYWGLRNNKTLTTTDVSRNFLITQQQSYDVLTYIYQEAQKQITATKRTLFNEHKRRI
                     SAFRILDIDKGILRTPKKEIAYASYDNIPTVTMKSTRCEIQDSFTELRRWMCSRKTGD
                     RVEVFLNA"
BASE COUNT          644 a          538 c          515 g          766 t
ORIGIN      
        1 tgtcaccgtc cgctatcttt tttaagacaa atattgttga gcctgtttca tatcaacttt
       61 gggagtatcc tgtgatacgt tttttagcag ttcactgata cctgcaaccg aactcaccag
      121 gctacttgaa tcaagaggca tcataaccag tttactgttt gatgcagtgc caattgcctg
      181 gagtgcttca gtgtatttct gcgcaataaa gtaattaaca gactgaacat caccttcagc
      241 gattgcatcc gacaccagtt ttgttgcgcg agcttccgct tctgcctgac gttcgcgtgc
      301 ttcagactga agaaatgctg attgccgttc cccttccgct ttcagtattt gagactgttt
      361 ttccccttct gccttcagaa tttctgactg gcggatacct tccgcttcaa gaatccgggc
      421 acgcttagtt cgttccgctt tcatttgcgc gttcatcgct tcagtcagtt cttttggtgg
      481 tttaacatcc cgaatttcaa tacgtgtgac cttaattccc cacgggtcag tcgcataatc
      541 gacaaccgtt aacagtttag aattgatgct gtcacgttga gaaagcatat cgtcgagatt
      601 cattccacca acgacagttc ggatattggt cataacaaga tttgaaatag ctgatgcaag
      661 attatcgact tcataagctg ccttcgctgc atcaatgacc tggacaaaac aaacagcatc
      721 gatagttacg tttgcattgt ctttcgaaat aacttcctgt ttaggaatat ccaggacggt
      781 ttccatcatg ttgatccgtt gacctatgcg gtccataaac gggatcagga agtgaagacc
      841 gggggaaagc gtatgggtat atttaccaaa gcgttccact gtccaggcat taccttgtgg
      901 cacgattttg acggcagatt taacgaatat cagcgcgact aaaattaata agagtaacgg
      961 aatagatgta actattggcg taattatagc gtcaatcatg attaatcctc tccccattca
     1021 tcacaatatg cttcaaccgg agtttttcct gcttcataat cagcacgcca tgcttccgca
     1081 tcggcggcat ttccaccgcg taactctgcg taatccatta atagctcgtg ccattcttca
     1141 aatgttacgg tttgtttagt ggaaccaaaa tcagccatat gtatttcctg cattttgtgg
     1201 aggtatctct ttgcaaatga aatctgaaat atgaacatca acagaaaaac actttcctgc
     1261 ttctttttct tttgagaagt tttttctgca ttcttcagcg gtcataggga agtcgctgta
     1321 atgagtccat ttcacggggc caccacctgg ttttttgatg gttgcgttta tttggtactt
     1381 aggcattcaa aaatacctcc actcgatcac ctgtctttct tgaacacatc cagcggcgca
     1441 gttctgtaaa actatcctgg atctcacaac gggtactttt cattgtgaca gttggtatat
     1501 tgtcgtagct ggcatatgca atctcttttt tgggtgtgcg caatatgcct ttgtcaatat
     1561 caagaatcct gaacgcgcta attcttcttt tgtgctcgtt aaatagtgtt cgtttagttg
     1621 ctgttatctg cttctgtgct tcctggtaaa tgtaggtaag cacatcataa gattgttgct
     1681 gtgtaataag gaaattacgg ctaacatccg ttgttgtgag cgttttatta tttcttaatc
     1741 cccaataagc cacgatgata tagagtggat atcctttcaa atctgccaca cattcaggaa
     1801 tgacaggatt aatttttaac ttacgcgcca tattttctcc cggttgtttg tctgaaatag
     1861 aaacatcccc ggaatacagc gtcataagct aattatgaaa agttgcagcc actcactaca
     1921 ggctctttat ctgattgttc atcatcatgc atagctaaac ccccttacct ggagtggaga
     1981 ctggcaaatc tggtgtactt ctgcgttgca gcctgtacga cttgctcagg gagagagtga
     2041 gtgagattaa ctcaacgcga gttcaccaaa taagggggtt taagtatgca gaatgatatt
     2101 tgcggataaa gagtcgttac ctgtgtagat ggcgcggcac gtcactctgt tacgtagccc
     2161 gttgctgaca gatgccagcg gtgcttgaaa tgcacgaata ataacctcct tatgccaggg
     2221 ctttaggcaa tgaccgctgt ccggggttgt taaagagcat gagatgacaa gcgttctaac
     2281 gggcgtcaaa gagtgctcat tttccaggca atgagcacaa aaaacgcgat gaacgagttt
     2341 tttacaatca tctggatagt attgtgtgtg cgacatttaa aacctgtcaa tgccacaaga
     2401 gcgatctaat gatctggata atatctaaaa aacagaataa tattctgttt gttaaatgat
     2461 gta
//
LOCUS       DAPGOF010000307         2460 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.313, whole genome shotgun sequence.
ACCESSION   DAPGOF010000307 DAPGOF010000000
VERSION     DAPGOF010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.313"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            43..687
                     /locus_tag="RHV13_004662"
     CDS             43..687
                     /locus_tag="RHV13_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV1244095.1"
                     /translation="MNNANGAVSIGNGLTVYGSVGLGTANALGGNSIVLGDNDTGFKQ
                     NGDGILDVYANSQRVFRFKNDAAFSLKKLLVGDSKTFSLSSSNTSTKNVTFNLWGAST
                     RPVVAELGDDSGWHFYSQRNTDNSITFAVNGQVSPSNYSNFDSRYALKTACVTSVRLG
                     AYKTHTMQKGTMFETAGYVITGLGIIGEVDGDDPARLRPLQYCINGTWYTAATA"
     gene            706..1251
                     /locus_tag="RHV13_004663"
     CDS             706..1251
                     /locus_tag="RHV13_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HDV1244096.1"
                     /translation="MINFKKFTSYTPDEDKSELVEAGVVFLRDENGNDWYECQKFFHE
                     NTIKIVYDSDNIVVSISNDVSTLWPVGASVAEIEILPEGVDINGNWMFDGKNVVERVR
                     TADELKAMAESRRTELLNNARQQLVISQTKLLRGRILSEDEQSSLDAWLDYIDAVNAL
                     DFNTITDKASFNAIVWPTEPG"
     gene            complement(1286..1810)
                     /locus_tag="RHV13_004664"
     CDS             complement(1286..1810)
                     /locus_tag="RHV13_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000983254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDV1244097.1"
                     /translation="MREHMNNFKNFAPYTPGEDKRELVDAGVLFLLDENGNDWYECQK
                     LFSECTKVIAYDSNNIVVSITDDASTLWPIGLSVAEVDSLPEDVDINGGWVFRDNSVV
                     KRIYSDTELQQQAESKKAALLSHAESVIVTLERAVKLNMATDEERAKLEAWERYSVLV
                     YRVDTAKPEWPEEP"
     gene            complement(1810..>2460)
                     /locus_tag="RHV13_004665"
     CDS             complement(1810..>2460)
                     /locus_tag="RHV13_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV1244098.1"
                     /translation="GDNLGTWNSLRPLTINNANGAVSIGNGLNVTGDIRTNAWVYANR
                     FSVNSSSGSWISMRDQNVIFGLNKVSTSSAQALLRQDHADRKFFIGGLGNSQFGFYMI
                     NNSRTSNGTDGQAFLDSSGNFQCGGQILPANYSNFDSRYTLKTACVTSVRMGSAASYK
                     PSSNGVSWTQNLGSGLVMTGIIVQETGSNSADNIGGIYYRPVQYCINGTWYTAASV"
BASE COUNT          718 a          500 c          499 g          743 t
ORIGIN      
        1 ggtgacaatc ttggaacatg gaatagttta agaccgctta ccattaacaa cgctaacggg
       61 gctgtttcaa tcggtaatgg tttaactgtt tatggttctg ttggcctggg tactgcaaat
      121 gctcttggtg gtaactctat cgtccttggc gataatgata ctggttttaa acagaatggt
      181 gatggcattc ttgatgttta cgctaatagt cagagagtat tccgctttaa gaatgatgca
      241 gctttttctt taaaaaaatt gctggtcgga gatagtaaaa ctttttcatt atcaagttca
      301 aacacgtcca ctaagaacgt aacctttaat ctatggggtg cctctactcg tcctgttgta
      361 gctgaattag gtgatgattc aggatggcat ttttatagtc agagaaatac tgataacagt
      421 ataacatttg ctgtaaatgg tcaggtgtcc ccctctaatt atagtaattt tgactcgcgc
      481 tacgcattaa agacggcttg tgtgaccagc gtcagacttg gggcatataa aactcatacc
      541 atgcagaaag gcacgatgtt tgaaactgct ggctatgtca ttacagggct tgggattatt
      601 ggtgaagttg atggtgacga cccagcaaga ttgaggcctt tacagtattg cattaatgga
      661 acttggtata cagcagccac agcatgatta ttaatggatc aaacaatgat taattttaaa
      721 aagttcacat cctacacccc agatgaagat aagtcagagc ttgttgaagc tggagttgta
      781 tttttacgtg atgaaaacgg taatgactgg tatgaatgtc agaaattttt tcatgagaac
      841 acgataaaga tagtttatga cagcgataat attgttgtga gtatttcaaa cgatgtttca
      901 acactctggc cagtgggggc atctgtagca gaaatagaaa tcctgcctga gggagtagat
      961 attaacggga actggatgtt tgacggaaaa aatgtggttg aaagagtaag aaccgcagat
     1021 gagttaaaag ctatggcgga atcaagacgc actgagctac ttaacaacgc acgtcaacaa
     1081 ctcgtgatta gccagacaaa attactacgc ggcagaatat tatctgaaga tgaacagtct
     1141 tctcttgacg cctggctgga ctatattgat gcagttaatg cattagattt caacacaata
     1201 acagataaag ccagtttcaa tgcgatcgtc tggcctactg agccaggtta aaagtgaagg
     1261 cccgataaat cgggccttca cttcactacg gttcttctgg ccattctggt ttagctgtat
     1321 ctacgcgata aaccaacacg ctataacgct cccatgcttc cagtttagct cgttcctcat
     1381 ctgttgccat attcaattta accgcccgct caagagttac aataactgat tcggcatgtg
     1441 aaagtaaggc agcctttttt gattcagctt gctgttgtaa ctccgtatca gaatatatgc
     1501 gtttaacaac agagttgtct ctaaacaccc aaccaccgtt gatatctaca tcttcaggta
     1561 aactatccac ttctgctaca gataatccta tgggccaaag ggttgaggcg tcatctgtaa
     1621 tgctgacaac gatattgttg ctatcatacg caattacctt tgtacattcc gaaaataact
     1681 tttgacattc ataccagtcg ttaccatttt catccaacag gaataaaact cctgcatcaa
     1741 caagctctct tttatcttca ccaggcgtat atggtgcgaa atttttaaag ttattcatat
     1801 gctctctcat tatactgatg ctgctgtgta ccatgtaccg ttgatacaat actgaaccgg
     1861 tcgataataa atcccgccga tattgtcggc tgaattactt cctgtttctt gaacaataat
     1921 acctgtcata accagtccgc ttcctagatt ctgagtccag gaaacaccat tgcttgatgg
     1981 tttataactg gcagcagatc ccattcttac gcttgtcaca caagccgttt ttaatgtgta
     2041 gcgcgagtca aaattactat agttagctgg aagtatttgc ccgccgcact ggaagttacc
     2101 actgctatca agaaatgctt ggccatctgt accatttgat gttcttgagt tgttaatcat
     2161 gtaaaagccg aattgactat taccaagacc accaataaag aactttctgt cagcatggtc
     2221 ttgtcttaat aatgcctggg cggaactggt tgatacctta ttaagaccaa aaataacatt
     2281 ttgatctcgc atactaatcc aagaacctga gctgctatta acagaaaatc tgtttgcata
     2341 tacccatgca ttggttctta tatcgccagt tacattgagt ccattaccga ttgaaacagc
     2401 cccgttagcg ttgttaatgg taagcggtct taaactattc catgttccaa gattgtcacc
//
LOCUS       DAPGOF010000308         2441 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.314, whole genome shotgun sequence.
ACCESSION   DAPGOF010000308 DAPGOF010000000
VERSION     DAPGOF010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.314"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(1..150)
                     /locus_tag="RHV13_004666"
                     /pseudo
     CDS             complement(1..150)
                     /locus_tag="RHV13_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031587700.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
     gene            228..500
                     /locus_tag="RHV13_004667"
     CDS             228..500
                     /locus_tag="RHV13_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1244099.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYQSVFTDFIAGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
     gene            481..1689
                     /locus_tag="RHV13_004668"
     CDS             481..1689
                     /locus_tag="RHV13_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HDV1244100.1"
                     /translation="MTRSGGDFQPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKML
                     ACGTRILGVKEYNCDKPECPHVRYVTNSCGSRACPSCGKKATDLWIATQLNRLPDCDW
                     VHLVFTLPDTLWPVFESNRWLLNDVCRLAVENLLYAARKRGLEPGIFCAIHTYGRRLN
                     WHPHVHVSVTCGGLNKHGHWKKLSFLKDAMRSRWMWNMRQLLLKAWSEGLAMPESLSH
                     ITTESQWRSLVLKAGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGA
                     SLSFRYLDHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVCGEKLPQV
                     YRALGMDKPEPVAKVCYAQMVKQFLSRDPFECVLCGGRMVYRRAIAGLNVSGLKKNAR
                     DISLLRYMPA"
     gene            2035..2280
                     /locus_tag="RHV13_004669"
     CDS             2035..2280
                     /locus_tag="RHV13_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1244101.1"
                     /translation="MEATKDSPDVKQELLNRLFSGKRMTGRDEMDICRLLRKNEEEKI
                     RQSELNIIRNFIDEQQLTNSDIPKKSTVPNEDKKGIR"
BASE COUNT          647 a          559 c          671 g          564 t
ORIGIN      
        1 atacactccc ttgtacggat agttttccga caacttcatg attacatatc ttgcggtttt
       61 gattattttt gctgcaagaa atacatactt caaacgaaag gtctttattt gctgtctgta
      121 ttctgaagag tccaaggaat caaacttgaa ccacggcagc ctggcggctg caaatcgtgg
      181 tgtggcagaa tatgagcttt cagagtaacc atccagaatg agttcccatg tcacacaacc
      241 tggagcacca gaaagtccac accagaatgg tgaaagaggt tctgaaagcc gtcgccaggg
      301 ccaataatca cccttatcag tctgtattta cagactttat cgccggtcat ccctcctgta
      361 cggtctgttt ctgggaaacc ttccacaaaa tgtacccgga ttcaccgtac gaatacgtca
      421 cgttctgcca tacctgccgt cgttttgatt tatacgaaac tgaagcggag atgaaggcgg
      481 atgacccgaa gtggtggtga ttttcagccc cgaccgctga aacgcctgtt cacagccaac
      541 cagtgctgga catccttcct ggatgcgggc ggcctgcgcg atatcgaggt tgaggctgtc
      601 acaaaaatgc tggcctgcgg cacgcggata ctgggtgtga aggaatacaa ctgcgataaa
      661 cctgagtgcc cccacgtcag atacgtcact aactcatgtg gcagccgtgc ctgcccgtcc
      721 tgcggaaaaa aggccacaga cctgtggata gcgacacagc tgaatcgtct tcctgactgc
      781 gactgggtgc acctggtctt caccctgccg gacacgctgt ggccggtgtt cgaaagcaac
      841 cgctggctgc tgaatgacgt gtgccgtctg gcggtggaga atctgctgta tgcagcccgg
      901 aaacggggtc tggaacccgg tatcttctgc gccatccata cgtatggccg tcgtctcaac
      961 tggcatccgc atgtgcatgt gtcagtgacc tgcggaggtc tgaataaaca cggacactgg
     1021 aaaaagctga gcttcctgaa agacgcgatg cgttcgcggt ggatgtggaa tatgcggcag
     1081 ctgcttctga aagcgtggtc agaaggtctg gcgatgccgg aatcgttgtc acatatcacg
     1141 acggaatcac agtggagaag cctggtgctg aaagccggcg gaaaatactg gcatgtgtac
     1201 atgtcgaaga agacagccgg agggcggaat acggcacgct acctgggtcg ttatctgaag
     1261 aagccaccaa tagcggcttc ccggctggcg cattacaacg gaggagcgag tctgagcttc
     1321 cgttacctgg accacaaaac gggagaaacg gcgacggaaa cgctgacaca gcgggagctg
     1381 gtcgcgaggc tgaaacagca catcccggag aagtttttta agatggtgag atacttcggg
     1441 ttccttgcca accgggtgtg tggagagaag ctgccgcagg tgtaccgtgc tctggggatg
     1501 gataagccgg aaccagtggc gaaagtgtgc tatgcacaaa tggtgaaaca gttcctgagt
     1561 cgtgacccgt tcgaatgcgt gctgtgtggc ggccggatgg tataccgccg ggccattgcg
     1621 gggctgaatg tatcagggct gaagaaaaac gcgcgggata tcagtctgct gaggtatatg
     1681 ccggcctgac acaggctaag tgtgcccggg aatcggtgca ggtataaaaa acagcctgtt
     1741 acggtataaa aatcgaccat gcagagagag aaaccaccgg agtggaggga aaagcagaaa
     1801 ggctcacgtg gcataaggcg tggacagcgg tatcgtctgg tttttcaatt tcctatacgt
     1861 gaactggcat cttggatttc ggttgttctg ctgattatat acctatcagg caagaatcac
     1921 gtgcttacag cagcttttct ctgctttgca tggacagcat tctgggttct tttcctttta
     1981 cattatttcc caaaagtttt ctttattctc gatcgtcaga gtctgctcaa cctcatggag
     2041 gccaccaaag actcaccaga tgtaaagcag gagctgctta accgcctttt ttccggaaaa
     2101 agaatgaccg ggcgggatga aatggatatc tgccgtcttc tgagaaaaaa tgaagaagaa
     2161 aaaatccgtc agagtgaact gaatatcatc aggaatttta ttgatgaaca gcagttaacg
     2221 aatagtgata ttccgaagaa aagtacggtt ccaaatgaag ataaaaaagg cataagatga
     2281 tatgaaatta ataaccaggc aagcagagga tggcgtggtt tcatcctaag attgataggg
     2341 gttaacctgt aagataaaag gaccgtgttt acacgacgtc tctgaagggc ttcgtttggg
     2401 aaacggacaa tatctaactg acgaggaaat taaataaggg t
//
LOCUS       DAPGOF010000309         2408 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.315, whole genome shotgun sequence.
ACCESSION   DAPGOF010000309 DAPGOF010000000
VERSION     DAPGOF010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.315"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            258..401
                     /locus_tag="RHV13_004670"
     CDS             258..401
                     /locus_tag="RHV13_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HDV1244102.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            414..623
                     /gene="cspE"
                     /locus_tag="RHV13_004671"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             414..623
                     /gene="cspE"
                     /locus_tag="RHV13_004671"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HDV1244103.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            789..1598
                     /gene="rlmA"
                     /locus_tag="RHV13_004672"
     CDS             789..1598
                     /gene="rlmA"
                     /locus_tag="RHV13_004672"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HDV1244104.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLNEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPDGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1595..2161)
                     /gene="mntP"
                     /locus_tag="RHV13_004673"
     CDS             complement(1595..2161)
                     /gene="mntP"
                     /locus_tag="RHV13_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HDV1244105.1"
                     /translation="MNITATVILAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT          634 a          587 c          586 g          601 t
ORIGIN      
        1 ataacttctc ccactggcct ggaacaactg aacttattga actatgttag aaactacgcc
       61 agtttaagta tctgcctgaa ctggcaaggt taagcacaat gatatatcgg cgcgtattcc
      121 gttgcataag tgtgcaaaaa aagtggaaga cgtatcgaga tttgtgcgtc tgatcgagac
      181 atgtttaaaa atggcttgcc ataattaacg ttgtatgtga taacagattt cgggttaaac
      241 gaggtacagt tctgtttatg tgtggcattt tcagtaaaga agtcctgagt aaacacgttg
      301 acgttgaata ccgcttctct gccgagcctt atattggtgc ctcatgcagt aatgtgtcag
      361 ttttatctat gttatgcctg cgggcgaaga aaacaatcta aggaattttt caaatggcaa
      421 agattaaagg tcaggttaag tggttcaacg agtctaaagg ttttggcttc attactcctg
      481 ctgatggcag caaagacgtg ttcgtacact tctccgctat ccagggtaat ggcttcaaaa
      541 ctctggctga aggtcagaac gttgagttcg aaattcagga cggccagaaa ggtccggcag
      601 ctgttaacgt aacagctatc tgatcgaatc cactgatctg aagtgtgaat acgcttcaat
      661 ctcgctataa agcctcgtcg aatgcgaggc tttttactat gctttatctt cgctcctggc
      721 gttcggatat ttgcccgccg cgtgattcgc gttacacttg cggcctttag tatcctgccg
      781 gagttgtcat gtcgttttcc tgtccacttt gccatcagcc tctttcgcgt gaaaaaaaca
      841 gctatatctg tccccaacga catcagtttg atatggcgaa agaagggtat gtcaatctgc
      901 tgcccgttca gcataaacgg tctcgtgatc cgggcgacag cgcggaaatg atgcaagcac
      961 gccgcgcatt cttagatgcc ggacattatc agccgctgcg tgatgcaatt gtcgcccaac
     1021 tgcgggaacg gcttaatgaa aaggccacgg cggtgctgga tattggctgt ggtgaagggt
     1081 attacacaca cgcatttgcc gatgcgttgc ccgaaatcac cacgtttggt ctggatgttt
     1141 cgaaggtagc gataaaagcg gcggcgaaac gctatccgca ggtcactctt tgtgtcgctt
     1201 ccagccaccg tttgccgttt tccgatacca gtatggacgc cataatacgt atttacgcgc
     1261 cgtgtaaagc agaagaatta gcacgagtag tgaagcccga cggctgggtc attactgcca
     1321 cgccgggacc gcgacatttg atggagctga aggggctgat ttacaatgaa gtacatcttc
     1381 atgcacctca tgcagaacaa ctggaaggtt ttacattaca gcagagtgat gagttgtgtt
     1441 atccgatgcg tcttcgcggt gatgaagccg tcgcattatt gcagatgacg ccgtttgcct
     1501 ggcgtgcgaa gccagaagtc tggcaaacac tggcagcaaa agaagtgttc gactgccaga
     1561 cggactttaa tattcacctc tggcagcgtt cttattaacc gtggaagtgc gtccagagga
     1621 tctggacgcc gatgccgatc agcaccagcc cgccgagaat ttccgctttt ttcccaataa
     1681 ttgagccgat aaagcgacca accatcatcc ctaatgtcga cataatcaag gttgcacaac
     1741 caatggccaa tgcggtcgcg ataatgttga cctgaaggaa agcaagacca acacccacag
     1801 ccatggcatc caggctggtg gcaatcgcgg tggttaccag tagccagaaa ccgtgccgac
     1861 ggcgcggctc ttcatcttca tcatctgcgc cacgaaaacc ctcaataatc attcgcccgc
     1921 cgaggaatat cagcagcaca aacgcaatcc agtgattcca ttcaaggaca aaccggctgg
     1981 ctaacatgcc cattccccag ccgatcagcg gcgtcagggt ttcgacagca ccaaaaataa
     2041 ggccggttcg caacgcttca gaaaatttcg gtttatggag ggtggcacct ttaccgattg
     2101 atgcagcaaa tgcatccatc gacataccaa acgcaagaat aacagtagca gtgatattca
     2161 tgacaacgtc ctgaccgggg tatccataaa acacatcatc gcccccagca aacatgcata
     2221 catgaacaac aagccgcgat gggttttcgc gttcatgcag gcatagcgat tgatgtgcat
     2281 atggtctcgc ctgactgaaa gtcttcagtc cgtacgtgcc acgttttgca acgagtatgt
     2341 tgacacgtac atttccggaa tctggaaatt ggctactccc caaatgaggg cgcaacctta
     2401 acatattt
//
LOCUS       DAPGOF010000310         2388 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.316, whole genome shotgun sequence.
ACCESSION   DAPGOF010000310 DAPGOF010000000
VERSION     DAPGOF010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.316"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            496..1527
                     /locus_tag="RHV13_004674"
     CDS             496..1527
                     /locus_tag="RHV13_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDV1244106.1"
                     /translation="MAGLKNTPYNAVHWSQLAPEEQIRFWEDYEAGRATTFLVEPERK
                     RTKRRRGEHSTKPKCENPSWYRPERYKALSGQLGHAYNRLVKKDPVTGEQSLRMHMSL
                     HPFYVQKRTYVGRKYAFRPEKQRLLDAVWPVLVSFSDAGTHTVGMSVSRLAREISPKD
                     SKGKVIPELEVTVSRLSRLLAEQVRFGVLGVSEETLWDRETRQRLPRYVWITPAGWQM
                     LGVDMVKLHEQQQKRLRESEIRQQLIREGVLREDEDISVHAARKRWYLQRSQDALKHR
                     RAKAAASKRARRLKKLPADQQIHEMAEYLRKRLPPDEAYFCSDDHLKRMAIRELRQLE
                     LTLAAPPPH"
BASE COUNT          648 a          638 c          616 g          486 t
ORIGIN      
        1 tcgtgagtag gataaaagtc gatcttttaa tccaaaagaa agatctaaag cagaaaaatc
       61 tgcttttaaa aatagatttt atttttttga cgcaggtcaa gattaactta ttagaggtat
      121 cagtgaggag gcactggaag agaagagatc gttgtaatgc ttttcaaatt aacgtaaagc
      181 gggtatattt cggctgttat tagctgtgca gagggtggca ctctgtggag caaagcggcg
      241 aaagccggac ggcagaatgc gccataaggc attcaggaga gatggcatgt acgggcagta
      301 agtcagaaga ctgaagatgt tccggaagcc ataaaaggaa aacccccact atctttctta
      361 cgaacttggc ggaacgacga aagatagtgg gggcctcaca gaatacgggt aaagtataat
      421 gaaaccgtac cagagattca accctgtgca gtgtataaat acacggcaca atcgctccgc
      481 cataagcgac agcttgtggc aggtctgaag aatactccat ataacgcagt acactggagt
      541 cagttagcac ccgaagagca gatccgtttc tgggaagact atgaagcggg aagggcgacc
      601 actttcctgg ttgaaccgga aaggaagcgc acgaagcgtc gtcgtggtga gcactccacc
      661 aaacccaaat gcgaaaatcc gtcctggtat cgtcctgagc gctataaggc gctgagcggg
      721 cagctcgggc acgcctacaa ccgtctggtg aaaaaggacc cggtgaccgg cgagcagagc
      781 ctgcgcatgc acatgtctct gcatcctttt tacgtgcaga aacgaacgta tgtcggtcgc
      841 aaatatgctt tccgtccgga aaaacaacgc ctcctcgatg ccgtctggcc ggttctggtc
      901 agcttcagtg atgcgggcac acataccgta ggcatgagtg tttcccgtct ggccagagaa
      961 atcagcccga aagacagcaa ggggaaggtt attccggaac tggaagtgac ggtctcccgc
     1021 ctttcccgtt tgctggccga acaggtacgt tttggtgtgc tgggtgtttc agaggaaacc
     1081 ctgtgggacc gtgaaacccg ccagcgtctg ccacgttacg tctggataac accggcaggc
     1141 tggcagatgc tgggcgtcga catggtaaaa cttcacgaac agcagcagaa acgactgcgt
     1201 gaaagtgaaa tccgccagca gctcattcgg gaaggtgttc tgcgtgagga tgaagatatc
     1261 tccgtacatg cggccagaaa acgctggtat ctgcagcgca gtcaggatgc gctgaaacac
     1321 cgtcgtgcga aagcagcagc cagtaagcgc gccagacgcc tgaagaaact gcctgccgac
     1381 cagcagattc atgagatggc agagtatctc aggaagcgtc tgcctccgga tgaagcctat
     1441 ttttgttccg atgaccatct gaagcgaatg gccatcaggg agttgcgtca gcttgaactg
     1501 acgctggctg ccccgccacc gcactagaca gcaccattcc ctcagcactg aatcatcacc
     1561 agcccctccg gggctttcgg cgctggttcc gctcagccca aaatccgcag taatcacctt
     1621 aaatcccctc agaggggcat atctgcccat aaaaccacgc atcagtcatc agaacatggc
     1681 cacgttgttt cagttatcca cataaatccg caaacaaaga actttaagaa gctgcaaacc
     1741 tgaaacagca aacctgcaat atagtcttaa ccccattatt taatcccctg cgttgcttcg
     1801 ccgcagggaa aatctttatc tctgagacca ctgtgaacaa atacaaagag gccttcgctt
     1861 gcagcggcca aggccgcgcc gctcagaatc taaaagcacc tcccacgctg acgcgcgggc
     1921 cccgaacctc accgttctga aaccacaaca aaaaaacatc aggaataaaa acaccacaca
     1981 aacgcagcac cgtgcccacc cctcataact gaaaagcgag gccgcccccg cccaaagggc
     2041 gggaacaaca tcgcttttaa ttatgaatgt tgtaactaca ttgtcatcgc tgccagtctt
     2101 ctggctggaa gtcctcagta cacgctcgta agcggccctt acggcccgct aacgcggaga
     2161 tacgccccga ctgcgggtaa acccttgtcg ggaccactcc gaccgcgcac agaagctccg
     2221 tcatgactga aaacgggtat ggtctagcag ggctggggat gggtaaggtg aaatctatca
     2281 atccgtaccg gcttacgccg ggcttcggcg gttttactcc ggtatcacat gtaaccacag
     2341 cgtgccgcct tccatgccgc tgacgcggca tatcctggtg acgatatc
//
LOCUS       DAPGOF010000311         2328 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.317, whole genome shotgun sequence.
ACCESSION   DAPGOF010000311 DAPGOF010000000
VERSION     DAPGOF010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.317"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..272
                     /locus_tag="RHV13_004675"
     CDS             <1..272
                     /locus_tag="RHV13_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV1244107.1"
                     /translation="TKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNALS
                     AIQTTVKPISEIARENGYKCPSRFTERFHNRFNITPREIRKASRE"
     gene            389..490
                     /locus_tag="RHV13_004676"
     CDS             389..490
                     /locus_tag="RHV13_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1244108.1"
                     /translation="MRTYNPNSLLPSQMQKCTCNSLHLAFDLCGGEA"
     gene            487..942
                     /gene="ybcN"
                     /locus_tag="RHV13_004677"
     CDS             487..942
                     /gene="ybcN"
                     /locus_tag="RHV13_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HDV1244109.1"
                     /translation="MNLSQDGIKLHRGNFTAIGRQIQPYLEEGKCFRMVLKPWREKRS
                     LSQNALSHMWYSEISEYLISRGKTFATPAWVKDALKHTYLGYETKDLVDVVTGDITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAVNIGCHLTIPQSCEFQLLRDKQEA"
     gene            942..1112
                     /locus_tag="RHV13_004678"
     CDS             942..1112
                     /locus_tag="RHV13_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1244110.1"
                     /translation="MATPLIRVMNGHIYRVSNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            1105..1395
                     /locus_tag="RHV13_004679"
     CDS             1105..1395
                     /locus_tag="RHV13_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HDV1244111.1"
                     /translation="MADLRKAARGWECQVRIPGVCNGNSETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTHFVDAAYAKECALEGMARTQVIWLKEGVIKA"
     gene            1392..1754
                     /gene="rusA"
                     /locus_tag="RHV13_004680"
     CDS             1392..1754
                     /gene="rusA"
                     /locus_tag="RHV13_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HDV1244112.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHVSAEGQAYRDNVARIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            1751..1891
                     /locus_tag="RHV13_004681"
     CDS             1751..1891
                     /locus_tag="RHV13_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HDV1244113.1"
                     /translation="MMFEFYMAELLRHRWGHLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            1977..>2328
                     /gene="quuD"
                     /locus_tag="RHV13_004682"
     CDS             1977..>2328
                     /gene="quuD"
                     /locus_tag="RHV13_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HDV1244114.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRL"
BASE COUNT          632 a          514 c          589 g          593 t
ORIGIN      
        1 gcacaaaatg gacagcaaac aaggttgctc gatatctcta catttctgtt tctacattac
       61 atcgccgtct agcaagcgag ggggtaagtt tccaaagtat actggacgat gtgaggttaa
      121 ataatgcgtt gtctgctata caaacgacgg taaaacctat aagcgagatt gccagagaaa
      181 atggttataa gtgtccttct cgttttactg aaagatttca taatcgtttt aatataacac
      241 caagagagat aagaaaagct tccagagagt aaaagtgttt taagaaggag caattctatc
      301 gattttgatt ttgggaaatc aacacggcat aattatgtca ccggagcctg aacaactccg
      361 gtgacttctg cgctaaacgg ggacgtttat gcgcacatac aatccaaact ctcttctccc
      421 ttcacagatg cagaaatgca cctgcaattc tttgcatcta gcgtttgacc tctgcggagg
      481 tgaagcgtga acctctcaca agacggcatc aaattacatc gcggcaactt caccgctatc
      541 ggtcggcaga tccagcctta tctggaggag ggcaaatgct ttcgcatggt gcttaaaccg
      601 tggcgtgaga aacgcagtct ttcccagaat gcactcagcc acatgtggta cagcgaaatc
      661 agtgaatacc tcatcagcag gggtaaaacg ttcgccactc cagcttgggt aaaagatgct
      721 ctcaaacaca catatctcgg ttatgaaacc aaagacctgg ttgatgtcgt aaccggtgat
      781 atcaccacta tccagtcgtt acgccatacc tccgatcttg ataccggaga gatgtatgtc
      841 ttcctgtgta aggttgaagc ctgggcggtg aatattggct gccacctgac tattccgcag
      901 agctgcgagt tccagctgct ccgcgacaag caggaggcgt aatggctaca ccgcttattc
      961 gtgtcatgaa cggacacatc tacagagtat caaatcgtcg taagcgtaag cctgagctga
     1021 agccatccga aataccaaca ctgctcggat ataccgctag cctggttgat aaaaaatggt
     1081 tgcgactggc agcaaggagg aatcatggct gatttgagaa aagcagcgcg tggttgggaa
     1141 tgccaggtaa gaatccctgg cgtatgtaat ggcaattctg aaacgtctgt actggcacat
     1201 atccggctgg ctggattgtg cggtaccggt atcaaaccgc cagacctgat tgccaccatt
     1261 gcatgttctg cctgccacga cgaaatcgac cgccgcacac attttgtcga tgctgcatat
     1321 gcaaaagaat gcgcgctgga aggtatggcg agaacacagg ttatctggct gaaagagggg
     1381 gttattaagg cgtgaatacc tacagtatca cattaccctg gcctccgagc aataatcgct
     1441 attaccgcca taatcgcggg cgcacgcacg tcagtgcaga ggggcaggca taccgcgata
     1501 acgtcgcccg aatcattaaa aacgcaatgc tggatatcgg cctggctatg cctgtgaaaa
     1561 tccgcattga gtgccacatg ccggatcgcc gtcgccgtga cctggataat ctgcaaaaag
     1621 ccgcttttga cgcactcacc aaagcaggtt tctggctgga tgatgctcag gtcgttgatt
     1681 accgcgttgt gaagatgcct gttaccaaag gtgggaggct ggaactgacc atcaccgaaa
     1741 tggggaatga atgatgtttg agttttatat ggcagaactt cttcgccacc gctgggggca
     1801 tctgcgctta tatcgtttcc ccggttctgt tttgaccgat taccgaatac tgaagaatta
     1861 cgccaaaacc ctgacaggag caggagtatg aagtcagaga taacaatcaa ctaatactgt
     1921 tttgttgatt tttgcttgta attggcgttc tggtctgatt tttgtggagt aagttgatgc
     1981 gtgatattca gatggttctt gagcgttggg gagcgtgggc ggctaataat catgaagatg
     2041 tgacctggtc gtccattgcc gccggtttta agggattaat tacttcaaaa gtaaaatctc
     2101 gcccgcaatg ttgtgacgat gacgcgatga ttatttgcgg gtgcatggcc cgtctgaaaa
     2161 agaacaacag cgatttgcac gatttattag tagattatta tgtagtcggt atgacattca
     2221 tgtcactggc aggtaagcat tgctgctctg atggttatat cgggaaaagg ttacagaagg
     2281 ctgagggcat aattgaaggg atgttaatgg cattagatat ccggttag
//
LOCUS       DAPGOF010000312         2321 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.318, whole genome shotgun sequence.
ACCESSION   DAPGOF010000312 DAPGOF010000000
VERSION     DAPGOF010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.318"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            359..1387
                     /locus_tag="RHV13_004683"
     CDS             359..1387
                     /locus_tag="RHV13_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDV1244115.1"
                     /translation="MQLAVISKRHWSELSPEEQVRFWQDYEAGIESSFLVPQENKGGT
                     TKRRRGEHSTKPKCENPAWFRPDSYKALGGQLGHAYNRLVKKDPVTGQYTLRMHMSLH
                     PFYVLKRQSVGRKYKFRPEKQRLLDAIWVVLVSFCDRGLHTVGMSVSRLAEEISPKDS
                     KGNVIPETAVTVSRLSRLLAEQVCFGTLGTSEKTIWDRESRQRLPKYVWITETGWKML
                     GVDLVKLQEQQRKRLAESEIRLQLIKEGVIREGEEISVHSARKRWYAQRSLDAIKFRR
                     EKAAKRKRANRLAKLPYDEQRNEIARFILKRMPPDEAYWCTKERLEQLVARDLRQLEL
                     ALTASPPH"
BASE COUNT          643 a          582 c          567 g          529 t
ORIGIN      
        1 gaaggggctt cagtagctgt tctgaaaacg attttgaagc tgtcagagat gtgtgctgaa
       61 tcaaaaagga aacgagccag gcttgaacat cgttgatcga ttgcgcccat gctgctaaat
      121 ttgcaggcaa gttttccgcg atttgtaaat gcagaaaacg aggtgctgaa attttgttca
      181 agaacgtaaa tttcagataa agaaaacccc cgtaatctcg tgttttccag gcagcgaaga
      241 ttagcggggg ctgacatcaa ggcagagcct tgagcttatt tatttcaggt atgcttgtta
      301 cctgaaatgg agtttaattc catgagaagg aaatatcaac tcttacagtg caagctaagt
      361 gcagcttgct gtgattagca aacgtcactg gtctgaacta tcaccggaag agcaggtccg
      421 tttctggcag gactacgaag cgggcattga aagctcattc ctggtccccc aggaaaacaa
      481 aggcggaaca acaaaacgtc gccggggtga acattccact aagccaaagt gtgagaatcc
      541 agcctggttc cgtcccgaca gctataaggc gctgggcgga cagttgggcc acgcttacaa
      601 tcgtctcgtt aaaaaagacc cggtaactgg tcagtacaca ctcagaatgc acatgtcgtt
      661 gcatccattt tatgtactga aacgtcagag tgtcggtcgc aaatataaat tccgaccgga
      721 gaaacagcgg ctgcttgacg caatatgggt tgtacttgtg agtttttgtg atcgtggatt
      781 gcacactgtc ggcatgtctg tctcccgcct ggcagaagaa ataagtccaa aagacagtaa
      841 gggaaacgtt attcctgaaa ccgcagtcac agtatcgcgc ctttcccgtc tgctggcaga
      901 gcaggtttgc tttggcacgt tgggaacgtc agaaaagacg atatgggatc gtgaatcccg
      961 ccagcgactg ccgaaatacg tctggatcac cgaaactggc tggaaaatgc tgggagttga
     1021 tctggtgaaa cttcaggaac agcaacgtaa acgccttgca gaaagtgaaa tccgtctgca
     1081 actgattaaa gaaggcgtta tccgcgaggg cgaggaaatc tctgttcact cggctcgtaa
     1141 acgctggtat gcgcagcgtt ctctcgatgc catcaagttc cgacgagaga aagcagcaaa
     1201 gcgcaaacgt gccaatcgcc ttgcaaaact gccgtatgat gaacagcgaa acgaaattgc
     1261 acggtttatt ctgaaacgta tgccgccgga cgaagcgtac tggtgcacta aagaaagatt
     1321 agagcaactg gtagccaggg atctacgtca gcttgaactg gccctgacag cttcgccacc
     1381 ccactagttt agctgcctga tacaatcggc actctcttca gccccttcgg ggcttttcgt
     1441 gttgcctgca tttcatgaaa ttcctcattt ttgacgaccg tttttcattt tgtcccgctc
     1501 tgacagtttt ttgatggcca caatcgggta tcggagctaa accgccttcg gttattaaca
     1561 aaaacccgca aacgaataac ttcagaaagc agcaaacgta gaagatcaaa cccgcaatat
     1621 attcttaacc cctgttcttt aatcccctgc gttgcttcgc cgcagggaaa atatttatct
     1681 ttgaaacaac tgtggataat cacaaaaatg ccttcgcttg cagcggctac cgccgcgccg
     1741 ctcagaatat aaaagtacct cccaccgctt cgcggcgggc atatggacat ctgcccaatg
     1801 aaaaacaaag cccctccaac cctccaaaaa acactgcaaa tactcagcaa cagcgcccaa
     1861 cccatcactg caaagcgccg ccgcccccgc ccaaagggcg ggaacaacat cgctttcaat
     1921 aatggatgtt gtaactaaga aatctcatcg ctgtcagcct tctggctgga agtaccaaat
     1981 acacgctcgt aagcggccct gacggtccgc taacgcggag atacgccccg actgcgggta
     2041 aacccttgtc gggaccactc cgaccgcgca caaaagctat ttcatggctg aaagcgggta
     2101 tggcttagca ggatggggat gggtaaggtg aaacctatca atcagtaccg gcttacgccg
     2161 ggcttcggcg gtttagctct gtgccatata agacaacaga gtgccgcctt acatgccgct
     2221 ggcgcggcat caaaaagaaa gccccccaat aacgggggga aatagcgttt tgatcaaaat
     2281 gataatgcta gtgtgcttct tgcgtgccat tccaggcaca a
//
LOCUS       DAPGOF010000313         2262 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.319, whole genome shotgun sequence.
ACCESSION   DAPGOF010000313 DAPGOF010000000
VERSION     DAPGOF010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.319"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            81..584
                     /locus_tag="RHV13_004684"
     CDS             81..584
                     /locus_tag="RHV13_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV1244116.1"
                     /translation="MNNIAPQSPVMRRLTQQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYDGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIGLYEHLGFQHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(630..1046)
                     /gene="rraB"
                     /locus_tag="RHV13_004685"
     CDS             complement(630..1046)
                     /gene="rraB"
                     /locus_tag="RHV13_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HDV1244117.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1208..2212
                     /gene="argF"
                     /locus_tag="RHV13_004686"
     CDS             1208..2212
                     /gene="argF"
                     /locus_tag="RHV13_004686"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDV1244118.1"
                     /translation="MSRFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
BASE COUNT          580 a          539 c          573 g          570 t
ORIGIN      
        1 ctttataagt cgcttgcgca cgtaagaaga gagtggtatt aaacaaatgt ataattactt
       61 tatttttatt gggttaaact atgaataaca ttgcgccgca atcacctgta atgcgtcgcc
      121 tgacgcagca ggataatccc gccatcgccc gcgtcattcg tcaggtatcc gccgaatacg
      181 gtcttaccgc tgataaaggc tacaccgtcg ccgatccgaa cctggacgag ttgtatcaag
      241 tgtatagtca gccgggtcat gcatattggg tggttgagta cgatggtgaa gtggtcggcg
      301 gcggtgggat tgcgccatta accgggagtg agtcggacat ttgcgaactg cagaagatgt
      361 attttcttcc ggctattcgc ggtaaagggc tggcgaaaaa actggcctta atggcgatgg
      421 agcaggcacg agagatgggc ttcaaacgct gctatctgga aacgaccgct tttttgaaag
      481 aagcgattgg tttgtatgaa catttaggtt ttcaacatat cgactatgca cttggctgca
      541 cgggccatgt agattgtgaa gtgcggatgc tacgcgaact ctaaaaaaat gcccgctagt
      601 gtgtgctatc gggcatttta atcaattaat tagtggcgaa caccgtcatc gtcttcatcg
      661 acaaaatctt catcgtcgcc gtcttcgcca ttgggatctt caaagtaagt gccccaaccg
      721 tcgtactcaa cgtcaaattt ctccgccagc gtcatcagtt gttcaacctg ggcatcgatc
      781 agatcggcat tcaacgcgca ctcgctgagg atgtcgcagc aaatcacgat atcaccatct
      841 tcaacttcca gctcttctgg atcggtcact tcgtaaccga gtttaaacgc ttcaactgct
      901 gctttttcca gggtttccag atcgtctgcg gaaagatggt gttcgatggt gtacagcgcg
      961 tccggatcgc tgccatcttc cagtaattct tcaataatca aacgtgtttc ttcacgctgt
     1021 tcttccagtt gttccgggtt tgccatggct cattcctctt aatgtcctgc cgatactctt
     1081 attgtcacac accactgaca ttgcctccac ctttgtgaca aagatttatg ctttagactt
     1141 gcaaatgaat aatcatccat ataaattgaa ttttaattca ttgaggcgtt agccacagga
     1201 gggatctatg tccaggtttt atcataagca tttcctgaaa ttactcgatt tcacgccagc
     1261 tgaactcaac agcctgctgc agttagccgc gaaactgaaa gccgataaga aaagcggcaa
     1321 agaagaagcc aaactcactg gtaaaaacat cgcgctcatc ttcgaaaaag actcgacccg
     1381 tacccgatgc tctttcgaag ttgccgcata tgaccagggt gctcgcgtta cttatctcgg
     1441 cccaagcggc agtcagattg gtcataaaga gtcgattaaa gacactgccc gcgtgctggg
     1501 tcgcatgtat gacggtattc agtatcgcgg ctatggtcag gagattgtcg aaacactggc
     1561 ggaatacgct ggcgtgccgg tatggaacgg cctgaccaat gagtttcacc ccacgcagtt
     1621 gctggcggat ctcctcacca tgcaggagca tttacccggt aaagcattca acgaaatgac
     1681 gctggtctat gcaggtgacg cacgtaacaa catgggcaat tcgatgctcg aagctgcggc
     1741 gcttaccggt ctggatttgc gtctggtcgc gccacaggca tgctggccgg aagctgcgct
     1801 ggttacggaa tgccgcgccc tggcacagca aaatggcggg aatattacgc tgacggaaga
     1861 tgtagccaaa ggagttgaag gtgctgactt tatctatacc gatgtatggg tgtcgatggg
     1921 ggaagcaaaa gagaaatggg cagaacggat tgcattactg cgtgattatc aggtgaacag
     1981 caagatgatg cagttgaccg gtaacccgga ggtcaaattc ctccactgcc tgcccgcgtt
     2041 tcatgacgac cagacgacgc ttggtaagaa aatggcggaa gagtttggcc tacatggcgg
     2101 aatggaagtg actgatgagg tcttcgaatc tgccgccagc attgtttttg atcaggcgga
     2161 aaaccgcatg cataccatca aagcggtgat ggtcgcgacg ctcagtaaat aattgatatc
     2221 gctataaaga tgccgggaaa tacaaaaaca tcccggcatc ac
//
LOCUS       DAPGOF010000314         2169 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.321, whole genome shotgun sequence.
ACCESSION   DAPGOF010000314 DAPGOF010000000
VERSION     DAPGOF010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.321"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(5..1528)
                     /locus_tag="RHV13_004687"
     CDS             complement(5..1528)
                     /locus_tag="RHV13_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDV1244119.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECNLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQNQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNSPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNYRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLEYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            complement(1860..2108)
                     /locus_tag="RHV13_004688"
     CDS             complement(1860..2108)
                     /locus_tag="RHV13_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1244120.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
BASE COUNT          651 a          471 c          459 g          588 t
ORIGIN      
        1 attttcactc aaccacaacc ttcaccttcg gtttagaaag gataattttg accaggtcga
       61 tgtatgtaac aggtttatag aaataataac cctgctgaaa tgtgatatta ttttggttca
      121 gatattcaag ttgttctttc gtttcgacac cttcagcgac gatactgatc gaaagtttac
      181 gcgccagatc caatacacaa tccagaattc gggttgattc tgggtctgcg ttgacgcggc
      241 caacgaaact gtgatcaatt ttgatataat caatatgcag gtcatgcaga taagaaagcc
      301 ctgagtagcc agtaccgaaa tcatccagcg cgatgacaaa accattttca tgcagaatgt
      361 tcaaccgctg aaccagactt tcatcaacat ttaatggctc acgctcggta acttccagaa
      421 cgaggtttaa atcgcggcgg gtaaaactgt cacggtaatt taagcactcg tcgacaaacg
      481 tcggcgaaat aatatgcgag gcgctaaaat taattccaat atgaaaacct tccggcaatt
      541 tactcgcgat agcgttcatc tgtctggcaa cctgattcat caggctttgc gtaagcggaa
      601 cgattaatcc ggatttttca gcaagtggaa taaatgccgc gggtgaaata tatccaccgt
      661 gaggttgttt ccagcgggct aacacctcaa ctccccgcaa tgtcccttcc cgaccattca
      721 ccactggttg gtaaaaaggg acgatctccc cattatttat agcccgtcgc aggatctctt
      781 caggcgttgc gcttttattg aaatatctat caagcaggaa ggcagcggcg caggcgatta
      841 acagaataaa tattaaaatc ccaaagcctt tatcgataag tctgtagaag ctaaagagtg
      901 gcggcgagtt aaagataata gtgaaatgat attttgtgga gtgaaccttc tcggtatagg
      961 gataatgtcc attaagtgtc atcacatcac ccgtaggccc aataatgtca tccgccacgc
     1021 gtaatacata ccttacccct ttcaagggta cattaagtgc cccacgaata tgctgatcgc
     1081 ttatcgtaac caaaatgcgc gtatctgcaa attggttctg atagagcagg ataggtaatc
     1141 tattaacggt gtcgattgct ggagccaaca gtaaattact gtccgggaaa acaggaatac
     1201 gagacaacag gacccgattc ccaggcaggg atgtacacca gactattccc tgtttgagaa
     1261 tgatgattgt gcgcaggtga ggtttcagag ctgcttcagt gccaagctga tattgctcct
     1321 cgaggttgca ttccttcccg gcaatgttaa ccgctgtttg agtcgcttgt gacgcttctt
     1381 caagtatatg attgatattg ttggcggcat atctcgctcc cgccaggtac tctgcttttg
     1441 cggaatacca gagctggatg ttcaaaatag cgacaccggt aataaacaag catatcgcaa
     1501 cgaggatggg tatgagtgta ttgcgcattt gcttgtcccg gccaaagggt ttaatacatg
     1561 aaaatgccat tcattcgctc gcgctggcat ctacagagag catggtagag agcataagaa
     1621 atatccacgc cgaagataaa attttctcca tcttgatcta acccattaat tattgagcat
     1681 aatgtaggca gtacaaaata agttaggcgg gatatcaggc gtcaagaatg gagagcgaga
     1741 actctccatt cttgacacct gatattgcgg acataataag aaagcataac gcctgaaatg
     1801 ctcactttgc atcagcatgg tgatacagct gatgtttatt ctaaaacctt actcaagttc
     1861 taagagagca cggattccct gtcattaata atgatatccg ttagtgtagt cttatgcatt
     1921 tcaatgtact gcaatatttg cagatagaga tcagaaagaa tcacactggt ttcatctttt
     1981 aaacgcaagc gtaaagcctg atcaataata tgactatcaa catacccatg cgtcatgaac
     2041 acctccctct cgagattttt aaacaaagca cattgaaaaa taaatctctc tgggattgaa
     2101 ttattcatgt cagcctcgta gaaatattaa cccaataaaa ttatgctgtg tatagtctaa
     2161 agcgacaga
//
LOCUS       DAPGOF010000315         2069 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.322, whole genome shotgun sequence.
ACCESSION   DAPGOF010000315 DAPGOF010000000
VERSION     DAPGOF010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.322"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..83)
                     /locus_tag="RHV13_004689"
     CDS             complement(<1..83)
                     /locus_tag="RHV13_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HDV1244121.1"
                     /translation="MTIEKHERSTKDLVKAAVSGWLGTALE"
     gene            complement(141..776)
                     /locus_tag="RHV13_004690"
                     /pseudo
     CDS             complement(141..776)
                     /locus_tag="RHV13_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
     gene            849..2057
                     /locus_tag="RHV13_004691"
     CDS             849..2057
                     /locus_tag="RHV13_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1414 family transposase"
                     /protein_id="HDV1244122.1"
                     /translation="MDEKQLQALANELAKNLKTPEDLSQFDRLLKKLSVEAALNAEMT
                     HHLGYEKNQSRPGANSRNGYSTKTVITGDGPLELRTPRDRDGTFEPQLVKKNQTRITG
                     MDNQILSLYAKGMTTREIAAAFKELYDADVSPALISKVTDAVMEQVVEWQNRPLDAVY
                     PIVYLDCIVLKVRQDSRVINKSVFLALGINIEGQKELLGMWLAENEGAKFWLNVLTEL
                     KNRGLNDILIACVDGLKGFPDAINTVYPKARIQLCIVHMVRNSLRFVSWKDYKAVTRD
                     LKAIYQAPTEEAGQQALEAFASAWDSRYPQISRSWQANWPNLATFFAYPTDIRKVIYT
                     TNAIESLNSVIRHAIKKRKVFPTDDSVKKVVWLAIQSASRKWTMPLKDWRMAMSRFII
                     EFGDRLDGHF"
BASE COUNT          563 a          502 c          489 g          515 t
ORIGIN      
        1 aattcaagcg cagtgcccag ccatcccgat accgctgctt tcaccaaatc cttagtgctt
       61 ctttcgtgtt tttctattgt cataatggtt atctctaaaa aagaggtaag atgcgtacta
      121 cttactcgcc gttattggta ttattcagaa aaagtgagta agactttgca gcaatgtttt
      181 tgatcctgtt caaataaact aatggcatca gcaacatgct ggaaatcaaa cgtatgggta
      241 attaattttt ctggtttaat taacccttta cttaaccagt cgataacgac cggaaattta
      301 tttgcattta agcgtgaaga gaaaatagag agttcttttc cggtaattcc ttgctgaatc
      361 acttcagacg gttcactgga gaaccccatc aatacaatac gtgccgctgg agaagccagc
      421 gttacggcct ctttcaagat agaaggatga caagccgcat cgataattaa tgtcggcttg
      481 atgccttttt cagtgaaaat ctcgccaagc ggtgtctggc tgttattaat cgcccagtca
      541 gccccgctct ctttcgcttt ttccagtcgt tcatcaatgc gatcggcaac aatcacattt
      601 ttaacgttat agacgccttt taatacctga acgatcgtca ggccgattgg accggcacca
      661 taaaccagaa cggtatcatt ttctgtcggt tgaccatgtc cggttacgtt agccgcaatg
      721 gtaaaaggtt cgatcattac cgcatattga tcggccactg cttcaggaat tttccagagc
      781 ctgtacatag atttgtgtaa ttgcctgatt ttgatatgtt caatccagca tcaaatgaag
      841 gttaatttat ggacgaaaaa cagttacagg ctctggctaa cgaactggcc aaaaacctca
      901 aaacccctga agacctcagt cagtttgatc ggctgctgaa aaagctcagc gttgaagccg
      961 ctctcaatgc agagatgaca caccatcttg ggtatgagaa aaatcagtcc agaccaggag
     1021 ctaactcccg caacggttat tccacaaaga ccgttatcac aggcgacggt ccactggaac
     1081 tgcgtactcc gcgcgatcgt gacggtacct tcgaaccaca actggtaaag aaaaatcaga
     1141 cccgtattac cgggatggat aaccagatcc tctcgttgta tgccaaaggg atgaccaccc
     1201 gtgagatagc cgctgcgttc aaagaactgt atgacgcaga tgtttcaccg gcactgatat
     1261 caaaggttac cgatgccgtg atggagcagg ttgtagaatg gcaaaaccga ccactggatg
     1321 ctgtttaccc cattgtttat cttgactgta tcgtcctgaa agttcggcag gacagtcgcg
     1381 tcatcaacaa atcggtgttc ctggcactgg gcatcaatat cgaaggtcag aaagaactgc
     1441 tgggtatgtg gctggccgaa aatgaagggg cgaagttctg gctcaatgtg ctgactgaac
     1501 tgaaaaaccg cggtctgaac gatatcctca tcgcctgtgt ggatggcctg aaaggcttcc
     1561 cggatgccat caacacagta tatccgaagg cccgcatcca gttatgcatc gtgcatatgg
     1621 tgcgcaacag cctgcgcttc gtgtcatgga aggactacaa agccgtcact cgcgacctga
     1681 aagcgattta tcaggctccc acagaagaag caggccagca ggcgctggaa gcgttcgcca
     1741 gcgcctggga cagtcgctac ccgcagataa gccgaagctg gcaggctaac tggccgaatc
     1801 ttgccacgtt cttcgcttat ccaacggaca tccgcaaagt gatctatacg acgaatgcca
     1861 tcgagtcgct aaacagcgtg atccgccatg cgatcaaaaa gcgtaaagtg ttcccgacag
     1921 acgactcggt gaaaaaagtg gtgtggctgg caatccagtc tgcgtcccgg aaatggacga
     1981 tgccgttgaa ggactggcga atggcaatga gccgctttat tatcgagttc ggtgaccgcc
     2041 tggacggtca cttctgagaa aggcattta
//
LOCUS       DAPGOF010000316         2064 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.323, whole genome shotgun sequence.
ACCESSION   DAPGOF010000316 DAPGOF010000000
VERSION     DAPGOF010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.323"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            238..1500
                     /locus_tag="RHV13_004692"
     CDS             238..1500
                     /locus_tag="RHV13_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1244123.1"
                     /translation="MSTSAQNQSIENVSIPDVLNAGIPAIIQNIRAAQRRVSCDDLTA
                     RFFDNAVQSAEMLHAQLIDVYNAEADSHNSLVDAAENMQLDLGLKGKEIEELQLQIEH
                     LKRQQQDAIDDATHDANQRADNAERISIELETKLNEMTAMVELRNSQISTLKSQYKEI
                     MKLDPFNLEKRYNKAKSERQELRKQVADLNQQLKKTIKDASEARVAFANKKAEVTALV
                     NENAKFATLKKEMYGITERRFPASKLHPTLGQISFFPRLLAYGISSPKEFNNERPYIV
                     SKLDFAYQFCCDMGYAIDIRINEWLMPNFQPLAIFREFQPEGWVEFFHELICKEMESR
                     RPELVRRVEWAQEVMLADAELPFEPEFIDDLATKGLHTLFDVVTRRHEQLVVELGLEE
                     TAARRLLDVCYARSDAWEKENGGTIYVR"
     gene            1573..>2064
                     /locus_tag="RHV13_004693"
     CDS             1573..>2064
                     /locus_tag="RHV13_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-phosphatase"
                     /protein_id="HDV1244124.1"
                     /translation="MSIFNKHAHQERPYIVIVDIDGTISEATEDRLHLLPPPGKGALT
                     KDWNEFNLACDTDTPITPVIDMVRQLFNVYTVWFVTGRCEIARDKTLAWLRKYVTNGA
                     EPLLSIRPATDDRNDGPAKIDLLKKIGLSKIAFALEDKIEVARVFRRHGVLTLMVREY
                     ENAL"
BASE COUNT          584 a          471 c          509 g          500 t
ORIGIN      
        1 aatctacaag gtgcactatg tggccattcc gacggaaata tcactactgg ctgatcgcct
       61 ttgttacgcc gaccggcggt atcaggcatg tcatcaccag gtatcgcaac aagagactca
      121 ccttagccag aattttacag gctgccatag gtgagggact ggatacaaat tgcgtagtcc
      181 ttcctccttc atacttagga aaaatgaccg aagcacaagc taatacggaa ctttgaaatg
      241 agcacttcag cacaaaacca atcaatcgaa aatgtatcta tccctgatgt cctgaatgcc
      301 ggtatcccgg ccattatcca gaacatccgg gccgcgcaac gccgcgttag ttgtgatgac
      361 ctcacagcac gtttttttga taatgcggtt cagtcagcgg agatgcttca cgcacagctt
      421 attgatgttt ataacgcaga agctgatagc cataactccc tggtagatgc agctgaaaat
      481 atgcagttgg atctcggtct gaagggtaaa gaaattgaag agcttcagct gcaaattgaa
      541 catttgaaac gccagcaaca ggacgcgatc gacgatgcga cgcatgacgc caaccagcgt
      601 gctgataatg ccgaacgtat aagcattgag ctggaaacaa aactcaatga aatgaccgcg
      661 atggttgaac tgcggaactc acagatttca acgctaaaat ctcaatataa agagatcatg
      721 aaacttgatc cttttaacct tgagaaacgc tataacaaag ctaaaagcga gcgacaggaa
      781 ctgcgtaagc aggtcgccga ccttaaccaa cagctcaaaa aaactattaa agatgcaagc
      841 gaagcgcgcg tggcatttgc taataaaaaa gcagaggtta ccgcgctggt taatgagaat
      901 gccaaatttg cgacgctcaa gaaggaaatg tatggcatta ctgagcgccg tttccctgca
      961 agcaaacttc atccgacgtt agggcaaatc tccttcttcc cgcgcctcct ggcttatggg
     1021 atctcatcgc ctaaagagtt caataacgag cgtccttata tcgtttctaa gctggacttt
     1081 gcttatcagt tctgctgcga catgggctat gccattgata tccgaatcaa cgaatggttg
     1141 atgccaaact tccagccgct ggccattttc cgcgagttcc agccggaagg ttgggtagag
     1201 ttcttccatg aattgatctg taaagagatg gaaagccgcc gcccggaact ggtccgccgt
     1261 gtcgagtggg cgcaagaggt tatgttggca gatgcagagc tgccgttcga accggaattt
     1321 attgatgatc tggcaactaa agggctgcat accctgtttg atgtggttac ccgccgtcat
     1381 gagcagttgg ttgtcgaatt gggtttagag gaaacagcgg caagaagact tctcgatgtt
     1441 tgctatgcac gtagcgatgc atgggaaaaa gagaacggcg gcactattta cgttcgctga
     1501 cagttacagt gtcactttta atgctggtgg agtgcgccca ccagcatttt tttcgtccaa
     1561 tgaggagggc atttgagtat tttcaataaa cacgcacacc aggaacgtcc gtatatcgtc
     1621 atagttgata ttgatggaac aatatcagag gcaactgaag acagactgca tttgcttccg
     1681 ccaccaggta aaggtgcatt aacaaaggac tggaacgagt ttaatctcgc ctgtgacacc
     1741 gatactccca tcactccagt tattgatatg gtgcgccagt tatttaacgt ttacacggtc
     1801 tggtttgtaa ccgggcgctg tgagatcgca agggataaaa cactagcctg gctgcggaag
     1861 tacgtaacaa acggggctga gcctttgcta tctatacgtc ctgccaccga tgacagaaat
     1921 gacggcccag caaagattga tctccttaag aaaattggtc taagtaaaat cgcgttcgcg
     1981 ctggaagata agattgaagt ggcgcgtgtt ttcaggaggc acggcgtgct tacgttaatg
     2041 gtcagggagt atgaaaacgc gctt
//
LOCUS       DAPGOF010000317         2033 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.324, whole genome shotgun sequence.
ACCESSION   DAPGOF010000317 DAPGOF010000000
VERSION     DAPGOF010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.324"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(1..927)
                     /locus_tag="RHV13_004694"
     CDS             complement(1..927)
                     /locus_tag="RHV13_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV1244125.1"
                     /translation="MMKIEPSILPSLAWFALIVRAGSFSRAASEMGITRAALSQNLKS
                     LEERLNTKLIYRTTRNMSLTEEGLRLYEVLVSALGQIDDALKDVGDTQLEPTGLLRIN
                     SSRVAARMLVEPHIGEFLTRYPKTKIELIMDDGLSNIIADGCDVGIRLEQGLDEHMTA
                     VPVSPLIKLVTVASPDYLKEHGIPETPQELSNHNCLRLRHKSSGALSAWEFSNVVGGN
                     EEFEIEVSGKYISNDDESMIRMALNGTGIIQHLDFAIAEHINAGKLQPILEDWAVSFP
                     GFYIYVSSRVRMPSKVRAFIDFMVEKRVKIES"
     gene            1084..2004
                     /locus_tag="RHV13_004695"
     CDS             1084..2004
                     /locus_tag="RHV13_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV1244126.1"
                     /translation="MKTVSIKHTYWDIAADIYFPDDFNSEKKYPAIISAHPIGSCKEQ
                     TSGSVYGAALAKAGFIVIAFDASFQGSSGGEPRNLEDPTMRVKDFSIVVDYLTSLPYV
                     DAGRIGVLGICGGGGYAINAAMTERRIKAIGTVTGANYGRLMREGFTAFNPIGALEAM
                     AQQRTDEANGAKLRVDDLLPSSPDAALEAGLTEIDLFEATEYYRSPRGCAPNGVNRSL
                     FSHQTVAVGWDAFHLAEVLLTQPIMVVVGDRVGAFGAYRDGCEIIGRAASKHKELVVV
                     EGYSHYDLYDKPEPVKQALDKLIPFYKTHL"
BASE COUNT          596 a          460 c          434 g          543 t
ORIGIN      
        1 ttaactttct atttttactc ttttctccac cataaaatca ataaaagcac gaactttaga
       61 gggcattctc acccgtgatg aaacataaat ataaaatcca ggaaatgaaa cagcccaatc
      121 ctcaagaatc ggctgtaact tgcccgcatt gatatgttca gcgattgcaa aatccagatg
      181 ttgaatgata cccgttccat tcagcgccat tcgtatcatg ctttcgtcat cattagaaat
      241 atacttacct gaaacctcta tttcgaactc ttcattaccc cccacaacat tagaaaactc
      301 ccatgcagag agtgcaccgc tacttttatg ccgcaaccga aggcaattat ggttactcag
      361 ttcctgaggc gtttcaggaa taccgtgttc ttttaaataa tctggcgatg ccacagtaac
      421 cagtttaata agcggagaaa caggaacggc agtcatatgc tcatccagtc cttgctcaag
      481 acgtatgccc acatcacagc cgtccgcaat aatgttagat aaaccgtcat ccataatgag
      541 ttctatcttt gttttggggt aacgagtaag aaactcgcca atatgaggct caacaagcat
      601 ccgtgctgca acacgggagg aatttattct caacaggcct gtaggttcaa gttgggtatc
      661 accaacatct ttcaaagcat catcaatttg ccccaaagcc gacaccagca cttcatacag
      721 acgcagtcct tcctcagtaa gcgacatatt gcgggtcgta cggtatatca gcttggtgtt
      781 aagacgttcc tcaagagatt ttaaattctg ggacaatgcg gcccgcgtaa tacccatttc
      841 agaagcggct ctggaaaaac tgccggcacg aacaatcagc gcaaaccagg caagagaagg
      901 caaaattgaa ggctctattt tcatcattgt aaacctaaac ttaacactgt gatagatatt
      961 agaccattga tgataaccaa tacaagtcat actattacgc cgttctcgat atgaacacga
     1021 atgccagttg cggataatag aaatgatatt taaatctgtt taggcaaatc cccggagctt
     1081 gaaatgaaaa ctgtttcgat aaaacacact tactgggata tagctgctga tatctatttc
     1141 cctgacgatt ttaatagtga aaaaaagtat cctgccatta tcagcgccca tccaattgga
     1201 agctgcaaag aacagacatc gggtagtgtg tatggtgccg ccctggcaaa agctggcttt
     1261 atcgtcattg cttttgatgc cagttttcag ggaagcagcg gtggggaacc gcgtaatctg
     1321 gaagatccaa caatgcgagt aaaagacttc agcattgtcg ttgattacct taccagctta
     1381 ccttacgttg atgccgggcg tattggtgta ctgggtattt gtggtggagg tggatatgcc
     1441 attaatgccg caatgacaga acgtcgaatt aaagctattg gtactgtcac gggtgcaaac
     1501 tacgggcgtc ttatgcgtga gggatttaca gcgtttaatc ctatcggagc actcgaagca
     1561 atggcgcaac aaagaacaga cgaagctaac ggcgcaaaac ttcgcgtaga tgatttactc
     1621 ccctcttctc cggatgcagc tcttgaagcg ggattaacag aaatagacct ttttgaagcc
     1681 acagaatatt atcgttcgcc acgaggctgt gcacctaatg gggtaaatcg ttcgctattc
     1741 tcacatcaga ctgttgccgt gggttgggat gcatttcatc ttgcagaagt gctgttaact
     1801 caaccaataa tggttgtggt gggcgataga gtgggcgcat ttggtgccta ccgtgacggc
     1861 tgcgaaatta ttggccgcgc tgcctcaaaa cataaagagc tggtcgtggt tgaaggttat
     1921 tcacattacg acctgtatga taagccagaa cctgtgaaac aggcactcga taaattaatt
     1981 cctttttata aaacacattt gtaataatct tacagggaga tcccttatct ccc
//
LOCUS       DAPGOF010000318         2014 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.325, whole genome shotgun sequence.
ACCESSION   DAPGOF010000318 DAPGOF010000000
VERSION     DAPGOF010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.325"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(657..1571)
                     /locus_tag="RHV13_004696"
     CDS             complement(657..1571)
                     /locus_tag="RHV13_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV1244127.1"
                     /translation="MLCAVQKNLHAARKVLSGITGNVPKNNKPKTIIFDLRFLSAIKQ
                     KVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACEAHSHRSVALAKTVAAPIMATG
                     VGSALMYATSSGPYYAQSLSGSPDIEMIKNRMAQLFTQLDASVRNKYSPAFDKWNELV
                     DKLNHERNTVPFACLTTILATAINETPEGDTNVAVVMGCKSAKDRTISIVLGNSMLQT
                     LFEKRLADDGKIENLFDQQGYFSCDSLTAEELMMLKDLFDIRVLHVSNKFNVGLQGNI
                     NTDVLQDSFFKNVDFIHESNSYTVGMGV"
     gene            complement(1584..2014)
                     /locus_tag="RHV13_004697"
                     /pseudo
     CDS             complement(1584..2014)
                     /locus_tag="RHV13_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          565 a          430 c          383 g          636 t
ORIGIN      
        1 tactcattaa tgtatgtgcg aaaattacgc cggaaaatta attaacgaaa gcggattttt
       61 gcatgttgtt ttgtttttgc gagacgtttt ccagaaataa taagagcgtt gcagttgttt
      121 catttattac tcaaaaatgg tttagttatc gcccaggatg agttatgggt gctatgaata
      181 tattatattc agggatattt atttgcataa tctgaatggt ttagccgaaa aacgtcagca
      241 tcctaacagc accaggattt taggtgaaat taatcttcca tcacagaccg gtcagtactg
      301 ccaccaaccg gtctttactt gctcatcgtt ataattttgt tggtaacagt tcgggaaaca
      361 aggaattcct tccggaacac ccccggcatc atccgatagc attgacgtca atcatcagcg
      421 cgacgatgag tgttttaatc gcatcatagg gtggcgacgt tcagcacgat tacgggtcat
      481 agcaaaactc ctgagcaggc ggccacaatg ccgctttcac tggagttctg aatgctggtg
      541 ctttctgtat ggcataacca tgcccctcgc agctaagtgc ctgcacacat ctctctttgt
      601 atgtcattgt tgcaatgcat tgtccgaatt ctgataatcc taatgagttt tactcgtcag
      661 actcccatcc ctacggtata cgagttagat tcatgaatga aatcgacatt tttaaaaaac
      721 gaatcttgta aaacatctgt gttgatatta ccttgcaacc caacattgaa tttgttagag
      781 acatgaagaa ctcgaatatc aaaaaggtct tttaacatca ttaattcttc ggctgttaat
      841 gaatcacaac taaaatatcc ctgttgatca aaaaggtttt ctatttttcc atcatcggcg
      901 aggcgttttt caaagagtgt ttgcaacatc gagttgccaa ggacaatgga tatcgtgcga
      961 tctttcgcag atttacaccc catcacaacg gcaacattcg tatccccctc aggagtttca
     1021 tttatcgccg tcgctaaaat ggtggtcaaa cacgcaaacg gaactgtatt tctttcgtga
     1081 tttaatttat ctaccagttc attccattta tcaaaagccg ggctatattt gttgcgcaca
     1141 gaagcatcca actgggtaaa caattgagcc atgcgatttt ttatcatctc gatatccggc
     1201 gaaccagaaa gactttgcgc ataatacggt cctgatgatg ttgcatacat taaagccgag
     1261 cccacacctg tcgccatgat gggcgcagcc acggttttgg caagggcgac gctgcgatgg
     1321 ctatgcgctt cacaggctaa aggaacatat tccactgact gcggtaattc cttacatgcc
     1381 tccctcatca ttgcggtatg ctttacaaac aacggttttt cactgcccaa aaaaactttc
     1441 tgcttaattg cactaagaaa acgtaaatca aaaatgattg tttttggttt attatttttt
     1501 ggtacatttc ctgttatccc gctaagaacc ttccttgctg catggagatt tttttgcaca
     1561 gcacataaca caattgacga agccttctct actgcgtcat tgattttaat tttaaaatcc
     1621 gcatgatata cgttgacggg caatacaagt atgtctggcg ctacatcttt ggctgagtat
     1681 ttattggtga tcgacggacg tgaatccacg agaaaactta gctgcaaatt atgctctata
     1741 ttaccagctg tttttgtggt gtgattaaac atagagatgc cacctctcat tctttcacac
     1801 atatcctcaa ctttttcact tcgttcttgt ggcaatttgg ctttaacgtt aatttgatga
     1861 tttttacccg tgcattcctt aactacaagg ggaatgttat ctaaagtgcc atacaggcta
     1921 ataacgtcac caggattttt atcatgtaag taagtttgat taatactcga agcattcaca
     1981 aagaggtcaa tattataact gtttataact tcac
//
LOCUS       DAPGOF010000319         1976 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.327, whole genome shotgun sequence.
ACCESSION   DAPGOF010000319 DAPGOF010000000
VERSION     DAPGOF010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.327"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..1503)
                     /gene="tadA"
                     /locus_tag="RHV13_004698"
     CDS             complement(<1..1503)
                     /gene="tadA"
                     /locus_tag="RHV13_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HDV1244128.1"
                     /translation="MFFSDADYPGKDISDVVYVSISEKDDKPVHVYIADNSKGQRAVQ
                     AYIGTLNVKYPGKVNITWTTLDVIASRYQDSERRSDSNSSQHLNSNQEKVISYLAKAN
                     NLGSSDLHITPGRDGSEFTYVEARVHGELEILDVIPRKEGLELLGAAYSGMSDVIKGT
                     QFDPAIPQDARIAENFLKPVNLFGARYSHYPCVGGVYAVFRLIKDDSEDIPTFEELGY
                     MPQQIQTIRRMLQRPEGIIVLSGPTGSGKSTTLRTASEAYLSTFGFNHNDNMRLPRKR
                     LFTIESPPEGRIPGAIQTAVRDSVDGWVDAIKSAMRLDPDAILNGEIRDHASALAAIK
                     ASMTGHLLLTTLHANDALNIIERLEMENIQARLIADPLLFIGLLSQRLVQKLCPYCKR
                     TYAEMADKLSTEERKLIEENCLPEQVRLRNLDGCEHCYKGVTGRTVIAEVVSPDARFF
                     QLYRQSGRLEAKSYWHYELGGITRNQHLLHLINHGLVDPLSAHFISPIDED"
     gene            complement(1528..>1976)
                     /gene="pilP"
                     /locus_tag="RHV13_004699"
     CDS             complement(1528..>1976)
                     /gene="pilP"
                     /locus_tag="RHV13_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HDV1244129.1"
                     /translation="SLAYATESESTPKDEIKYSSPTILQVEQIQAETVIYEAKLARQK
                     AVSELRNLDLNGVASATPAITPSSGYSSLADTDIPAQRVAAKSLRVIEIFGTPQQMSA
                     RISLLDGSVTDVKVGQSIPGTSYKVASITGNGVWIQNGTEKRQLIL"
BASE COUNT          495 a          465 c          462 g          554 t
ORIGIN      
        1 atcttcgtca atgggggata tgaagtgtgc ggacagcgga tctaccaggc catgattaat
       61 caggtgtaat aagtgctgat tacgggtaat tcctccaagt tcatagtgcc agtatgattt
      121 tgcttccaga cgcccggact gacgatataa ctgaaaaaaa cgtgcgtcgg gtgagacgac
      181 ttctgctata accgtccttc ccgttacgcc tttataacag tgctcacaac cgtcaagatt
      241 gcgcaaccgg acctgttccg gcaggcaatt ttcctcaatg agtttccgtt cttctgtact
      301 cagtttgtct gccatttcag cataagtgcg cttacagtaa ggacagagtt tttgaacgag
      361 ccgttgactc agcagaccga taaacagaag aggatctgca attagcctgg cctgaatatt
      421 ctccatttca aggcgctcaa taatattcag ggcatcgttg gcgtgtaagg tcgtcaacag
      481 aaggtggcct gtcatcgagg ctttgattgc tgctaatgcc gatgcgtgat cacgaatttc
      541 gccgttcagt atcgcgtctg gatcaagtcg catagcggat ttaatcgcgt caacccagcc
      601 atcgacggaa tccctgactg ccgtctgtat tgcgccggga atgcggccct ccggcggtga
      661 ttcaatggtg aaaaggcgtt ttcgggggag acgcatatta tcgttatggt tgaaaccaaa
      721 cgtgctcaga taggcttctg aagccgttct cagagtcgtt gacttaccgg accctgtagg
      781 gccggataaa acgataattc cttccgggcg ttgtaacatt cgcctgattg tctggatttg
      841 ttgcggcatg tagccaagtt cttcgaaagt gggaatatcc tcgctgtcat ctttaatcag
      901 gcggaatacc gcgtacacac cacccacgca cggataatgc gaatagcgcg ccccaaaaag
      961 atttacaggc ttcagaaaat tctctgcgat acgtgcatcc tgcggtattg ccgggtcaaa
     1021 ctgcgtacct ttgataacat cgctcattcc agaataggca gcacccagca attccagccc
     1081 ttctttacgc gggatgacat ccagaatttc cagttcccca tgcacgcgtg cttcgacata
     1141 agtgaactcg gaaccgtccc gaccaggtgt gatgtgcaaa tcggaacttc ccaggttgtt
     1201 tgctttcgcc aggtagctaa tgaccttttc ctggttgctg tttaagtgtt gtgatgagtt
     1261 tgaatcagac cttctttcgc tgtcctggta gcgggatgcg ataacatcaa gagtggtcca
     1321 ggttatattt acctttccgg gatatttaac attcagagtg ccaatataag cctgaacagc
     1381 acgctgtcct ttactgttat cagcgatata aacatgaaca ggtttatcgt ctttttcaga
     1441 tatactgacg tatacaacat cagaaatatc ttttcctgga tagtctgcgt cagaaaaaaa
     1501 cattatgtgt cgtctctttc aatagcatta aagaataagc tggcgtttct ctgtaccatt
     1561 ctgaatccag acgccattac cagtgattga tgccacctta taagaagtac ctggaatact
     1621 ttgcccgacc ttgacatcag ttactgaccc gtccaggaga gaaattcgtg ctgacatttg
     1681 ttgtggtgta ccaaatattt caataacacg aagtgattta gcggctacac gttgcgctgg
     1741 tatatcggta tctgcaagag aagaataccc ggaagagggc gttattgctg gcgttgcgga
     1801 cgctacacca ttcaaatcaa gattacgcaa ttcactgaca gccttctgac gcgccagctt
     1861 tgcttcgtaa attaccgttt cagcctgaat ctgctctacc tgtaaaatgg tcggagatga
     1921 atattttatt tcgtcttttg gtgtgctttc agattctgtt gcgtaagcaa gtgaac
//
LOCUS       DAPGOF010000320         1876 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.328, whole genome shotgun sequence.
ACCESSION   DAPGOF010000320 DAPGOF010000000
VERSION     DAPGOF010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.328"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(81..1493)
                     /gene="pykF"
                     /locus_tag="RHV13_004700"
     CDS             complement(81..1493)
                     /gene="pykF"
                     /locus_tag="RHV13_004700"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HDV1244130.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT          466 a          441 c          462 g          507 t
ORIGIN      
        1 tattgcttct ggttatcgat taaataaaaa aagcgcccat cagggcgctt cgatatacaa
       61 attaattcac aaaagcaata ttacaggacg tgaacagatg cggtgttagt agtgccgctc
      121 ggtaccagtg caccagaaac cataactacg acgtcacctt tgtgtgccag accgctctgc
      181 agcgccagtt ctttacccag acggtagaag tcatcagtag aagtgatctc tttaaccagc
      241 tgcggcacaa cgcctttgct cagtaccaac tgatgagccg ttttttcgtt ggtggtcagt
      301 gccaggatgg tggcatccgg gaagtattta cgtactgcgc gagcagattt accgccctgg
      361 gtagcaacca cgatcagcgg agcatccagt ttttcagcag tttcaacggc accacggcat
      421 acagcttcgg taatgcgcag tttacggttg tcattgttga actcgagacg gctgttcatc
      481 acgcggtcgg tacgttcgca gatggtcgcc atgatagaaa ccgcttccag cgggtattta
      541 ccttttgcgg attcaccaga cagcatcact gcgtcagtac cgtcgaggat ggcgtttgca
      601 acgtcaccgg cttctgcgcg agtcgggcgt gggtttttga tcatggaatc cagcatctgg
      661 gtcgcagtga taacgacttt acgtgcacgg atacattttt cgatcatcat cttctgggcg
      721 aagataactt cttctaccgg gatttctaca cccaggtcgc cacgcgcaac catgatgccg
      781 tcagaggctt cgaggatttc gtcgaagttg ttgaggcctt cctggttttc gattttggag
      841 atgatgtgga tgttttcgcc gccgtgcgct ttcaggtgct cacggatttc gataacgtca
      901 gaacgcttac gaataaagga agcagcgaca aagtctacgc cttgttcgca accaaagatc
      961 aggtcctgtt tgtctttttc agccagtgct ggcagggcaa tggaaacgcc aggcaggttc
     1021 acacctttgt tttcgcccag gtcgccgttg ttcagcactt tacagataac tttgttacct
     1081 tcaatggcag taacttccat accgatcaga ccatcgtcaa ccagtacggt gttgccaaca
     1141 gacaggtcag ttgtgaaacc ttcatacgtt accgcaacca tttcgctgtt gccgataaca
     1201 gatttatcag tagtgaaagt aaaggtctga ccagctttca gagaaacgtc gttaccgcct
     1261 tccagtttca tggtgcggat ttccggacct ttggtatcaa gcaggatagc ggcggtttta
     1321 ccagttttgc tcatcacgtt gcgcagattc tgaatgcgct gaccgtgttc tgcatagtca
     1381 ccatgagaga agttcagacg cataacgttc atgccagcgt ccagcatttt agctaacatc
     1441 tcttcagatt cggttttcgg tccgatggtg caaacaattt tggtcttttt catgacagtc
     1501 ttagtcttta agttgagaag gatgggagaa acttgctttc tgggcgcact gccggaaagc
     1561 gaatccggta ttacgaaagt ggtggtgcct cgctctaagg ataggtgaca tcgaaaaagc
     1621 gtgcagagga atgtgtgctt gtgtctcagc ccaacgtgag tgcctgtttt tgagtcgttg
     1681 agttctacag tccaaagtgc tgaaaccatt caagagtcaa ttggcgcgca ttatacgctg
     1741 aaattaatcc aaaaggaaaa caaaaacagc gtttcaaaaa atatacaact tgttagacaa
     1801 taaattgtta tcaaagattt aacgttccag ggaaaaggtt acgcaaaaac ttcgctgttt
     1861 gcgcaacgtc tggtgc
//
LOCUS       DAPGOF010000321         1855 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.329, whole genome shotgun sequence.
ACCESSION   DAPGOF010000321 DAPGOF010000000
VERSION     DAPGOF010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.329"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>1855
                     /locus_tag="RHV13_004701"
     CDS             <1..>1855
                     /locus_tag="RHV13_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HDV1244131.1"
                     /translation="IRNAGKTNEYLAVKLPGDKKYTNLKSPLFRQNYIETRTLTLEKP
                     THKEIEKRLNTWLNKTSHEIKHIFNQAEKTRELYKTLSPSQQIDFLQERIKEYDSREK
                     LNERNSQQTSGREGGYKSCPKKFARIRQSEATVGLSRMPQRGMVYGINGFTRPDSVSV
                     LSDISQRDLAEQLPQREHPGQDVRRDYDRQFTESGIKSLERSSFLCETMFQTLNEAAE
                     KNEITTMAEIRRNIDPVRFLSSAAERFNIIPAQHKIRTAKDGSPRFSVGNRNMNASDF
                     LTKHINLAWKDAKSFLLEVYSQQLENTPYTRYPTYRRLTHHEARERLNSLNLSEKTLR
                     NTIRFERGKLYNDLREMRRELKLIPREQRDIAVGVIVYKKLTTLERLSELDTDGRHII
                     RQYHADWHKDKDEMKALERLKSYLNFDEINAISADEPELSLQKAVDSQRRLEEAKKVN
                     TKLKDLVMDKQDSRIVYRDQESEKPVFTDKGNFVVAGKNPSKEEIGIMLEYSREKFGG
                     VLKLTGSEDFKKMCAEVAAEQDMKIILRPEQYQQMMLELKAELQGNKFEQVETQENSQ
                     ESESRIEKGDALKEQATEQEQATEQAQTTEQAQATEQAQVAEQAQVAEQAQV"
BASE COUNT          636 a          374 c          423 g          422 t
ORIGIN      
        1 tataagaaat gctggtaaaa ccaatgagta tctggctgta aaattgcctg gagataaaaa
       61 atacacaaac ttaaaaagtc ctctttttcg tcagaattac attgagacaa ggacattaac
      121 acttgagaaa ccaacacaca aagagataga aaagagactt aatacctggt taaataaaac
      181 aagccatgag attaagcata tttttaacca ggcggagaaa acaagggaac tttacaagac
      241 attaagccct tcacaacaaa ttgatttcct gcaagagagg ataaaagaat atgactcaag
      301 agaaaaactt aacgaaagaa attctcagca aacgtcagga cgagagggag gttacaagtc
      361 gtgtcctaaa aagtttgcca gaatccgtca atctgaagca acagtcggat tgtcacgtat
      421 gccccaacgc ggtatggttt acggaatcaa tggattcaca agacccgact ctgtcagtgt
      481 attgtcagat atttcgcagc gtgatctggc agagcaatta ccgcaaagag aacatcctgg
      541 tcaggatgtg cgacgggatt atgatagaca attcacagaa tcaggaataa aaagtcttga
      601 acgctcttct tttttgtgtg aaacgatgtt tcagactctg aatgaggcgg cagagaaaaa
      661 cgaaattaca acgatggcag aaattcgcag aaatattgat cctgtcaggt ttctgtcttc
      721 tgccgccgag cgttttaata ttattccagc gcagcataaa atcagaacag caaaggacgg
      781 ttccccccgg ttttctgtgg ggaatcgtaa tatgaacgca tctgattttc tgacaaagca
      841 tattaatctt gcctggaaag atgcgaaatc ctttctgctt gaagtttatt cacagcagtt
      901 agaaaacacg ccatatacgc gctatccaac ctacagacgc cttacgcatc atgaagctcg
      961 tgagcgtctt aactcactga atttatctga aaaaacatta cgaaatacta tcaggtttga
     1021 gcgtggcaag ctgtataatg atctgcgaga gatgagacgc gagttgaagt taataccacg
     1081 tgaacagcgt gatattgccg tgggtgtgat tgtttacaaa aaactaacca cacttgagcg
     1141 tctttctgaa ctcgacacag atgggcgaca cattattcgt caatatcatg ctgactggca
     1201 taaggataaa gatgaaatga aagcccttga acgtctcaaa agctatctca acttcgatga
     1261 aatcaacgcc atttctgctg acgaacccga actttcgctt cagaaagcgg tggattccca
     1321 gcgccgctta gaagaggcga aaaaggttaa caccaaacta aaagatcttg tgatggataa
     1381 gcaggattcc aggattgttt atcgcgatca ggaatcggaa aagcctgtct ttaccgacaa
     1441 agggaacttt gtcgttgcgg gtaaaaatcc ctcaaaagaa gagatcggaa taatgcttga
     1501 gtattccagg gaaaagtttg gtggtgtact caaacttacc ggctctgaag atttcaaaaa
     1561 aatgtgcgct gaagttgccg cagagcagga catgaagatt attttacgcc cggagcagta
     1621 tcaacagatg atgctggaat taaaagcaga acttcagggt aataagtttg agcaggtgga
     1681 aacacaggaa aatagccagg agtccgaatc cagaatagag aaaggtgatg cattgaagga
     1741 acaggccacc gaacaggaac aggccaccga acaggcacag accacagagc aagcacaggc
     1801 caccgagcaa gcacaagtcg cggagcaagc acaagtcgcg gagcaagcac aggtc
//
LOCUS       DAPGOF010000322         1832 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.330, whole genome shotgun sequence.
ACCESSION   DAPGOF010000322 DAPGOF010000000
VERSION     DAPGOF010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.330"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(52..774)
                     /locus_tag="RHV13_004702"
     CDS             complement(52..774)
                     /locus_tag="RHV13_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1244132.1"
                     /translation="MDNFSFNDFIVDLKTMFNNKSASLSNRLMAADREQEKVISDANE
                     YHEFVLSLVESMLTNPVVRRRFDDQVYRMSFDEPDTVSETISELVFLADVNWQLGLGL
                     LNTNKNEAIKCLLLAIEYLDYCRGLEDQELWQQQQTTKLDVPAQGGRSKAARFDPIKA
                     KIIRLLQEACPSDGWDTKSEALKSIENGINELKWPSARDQNNLPKTGAEIFAMKIRQV
                     EVWSSQDQKIKAAFDSVVKPKK"
     gene            889..1116
                     /locus_tag="RHV13_004703"
     CDS             889..1116
                     /locus_tag="RHV13_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HDV1244133.1"
                     /translation="MEPKSVKILRVPALVKKLGIGRSTVYDWLDPSSDRHDPTFPQRV
                     KLGAKTVGWLESEIDAWVLSKVKKPLTPVSP"
     gene            complement(1170..1543)
                     /locus_tag="RHV13_004704"
                     /pseudo
     CDS             complement(1170..1543)
                     /locus_tag="RHV13_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714554.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
     gene            complement(1523..1759)
                     /locus_tag="RHV13_004705"
                     /pseudo
     CDS             complement(1523..1759)
                     /locus_tag="RHV13_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004874600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          479 a          435 c          407 g          511 t
ORIGIN      
        1 agttttggct tttaaggctt aaactgccag catattagac ctcaagtaca tctatttttt
       61 aggctttacg acactatcaa atgcagcttt aattttctga tcctgacttg accagacctc
      121 aacctggcgt atcttcattg caaatatttc agctccggtt ttgggcagat tgttctgatc
      181 acgtgcagat ggccatttca gttcattaat cccattctct atgctcttca aagcttctga
      241 tttagtatcc catccgtctg aggggcaagc ttcctgcaaa agcctgataa ttttggcttt
      301 aatgggatca aagcgagcag ctttacttct acccccctgc gccggaacat ctaattttgt
      361 ggtctgttgc tgttgccata attcttgatc ttcaagacca cgacagtaat caaggtattc
      421 aatagctaag agtaggcact ttatcgcttc atttttattc gtattcagaa gcccaagccc
      481 tagctgccag ttaacatcag ccaggaaaac aagctcactt atagtttcgc taaccgtgtc
      541 tggttcatca aatgacattc tgtagacttg atcatcaaac ctacggcgta ctaccgggtt
      601 cgtaagcatg ctttcaacca gcgatagtac aaactcatga tattcatttg catctgaaat
      661 aaccttttcc tgctcccggt cagccgccat gagtctattg gacaagcttg cgctcttatt
      721 attgaacata gttttcaaat caacgataaa atcgttaaaa ctaaaattat ccattattca
      781 atcagttaag ctccgtaaaa aatgaagtac tgcagccgta tgtcgcacag ttgcatcagt
      841 agatagtctg acttccatcc taagcaaacc aagagaggaa gctctttaat ggaaccaaag
      901 tctgtaaaaa tccttcgtgt acctgcactt gtcaagaagt taggcattgg gcgttcaacg
      961 gtatacgact ggcttgaccc tagttctgat cgtcatgatc cgacatttcc ccaaagggtg
     1021 aaactgggag caaagacggt ggggtggcta gaatccgaga ttgatgcctg ggttttgagc
     1081 aaagtaaaaa aaccgctaac gcctgtttcg ccatagtttg gaagattata tgggagagag
     1141 tgagtaccac actcttccca ttagctttgc agacgtgagc cctgtaggac taaaaccaag
     1201 gggcatcacc tccgcctgac ggcgagtatc ttgagcattg cggtaatcaa tgttcagcca
     1261 agggtcttgg taaagcgctt cactgaccgc gtcaaacatg ccttcctgga tcaccggtgg
     1321 taggagcggc tgactggccg cgaccacccg caccttctgc ggccattccc actctctcag
     1381 cgcccgtttg ctgtggctag gaagtgtgtc ccgcttgact aaaaaacggc gaaaatgaag
     1441 ccatcagatg tgcgggtaac agatgcttta actgcgcctg cgccacttgc atcagcggtg
     1501 attctgctgg cgatagctgc gccaagctca ggcctcgcga acctagcgga accgtatggt
     1561 cccggttcat cttcattcga gctgcaggaa ttttccattc tttagcttca aaatcgatct
     1621 catcccaacg agcttctgcc gcttctgcgg gtcgtgagat agttagaagt tgccacatga
     1681 acaagcatcg agtaggcaaa ataatgttgg catttcgcat tgtctgcatt agctgtggta
     1741 actgatcagg gcggatagcg gaggatgaaa atgtgaaagc cgcttttgaa gaagtgatta
     1801 ttggcaaaca gtgatttgcg ctaaagttat gt
//
LOCUS       DAPGOF010000323         1829 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.331, whole genome shotgun sequence.
ACCESSION   DAPGOF010000323 DAPGOF010000000
VERSION     DAPGOF010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.331"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            47..1828
                     /locus_tag="RHV13_004706"
     CDS             47..1828
                     /locus_tag="RHV13_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopB/SseC family type III secretion system
                     translocon subunit"
                     /protein_id="HDV1244134.1"
                     /translation="MSAPITGQTITFEQISETLRTQYSDAEKRLQDSSKTQVDPMRLN
                     KNPKSLDNDIRARLENKPMLAPPAMQVSDSDNATTAKTNDARLTMILGNLTGIADQDI
                     ATRLHNNLESTLLRHEMAHNKFRELSDAYASSLDSAQKADDIMHQANNNYNAVDKKVQ
                     SLEKKVNTLNQELSQLQPGDPQYNKVLTQKNAAEKTLTLSLQKKSLAEKSLNTAIMDA
                     DAAIGQSMEIFDEIQQQEQINNFTTNICLTQENQKNRNATATFILLITSVMEVIGDTN
                     CDSIKNQSEVMKEINHVRENKLNETARSYTTWTKVLNIINEVVTVVTFAVSAALIVIG
                     IVAAVPSAGASFAGALALIGGIAGAVVLGVDIICQITLGTTATGWILGKLTEGLSGII
                     SKYDPTLLAICALLDALGVQQTTIDLVQDIYSSAAASIVMVTLMIGATLICSVAISEL
                     VKALSKTAAEEITKEITDSIKSTVESMIKSVSKKIMSALNFLGDKIKASSQFIMIFAK
                     ITILLEKVAAGVCTIITSCMNQYAASKSADIEILQQDISNLSKTREQMLTVMQRIDKT
                     VEQEVAQMVRVLQHRTEALKFASHSIV"
BASE COUNT          634 a          371 c          362 g          462 t
ORIGIN      
        1 aaaagaatta attacacccg gtgatagctt aaaaaggagg tgaattatgt cggcaccgat
       61 cacgggacaa acgatcacgt ttgaacaaat cagcgaaaca ttgcgtacac agtatagcga
      121 tgcggaaaaa agactgcagg acagcagcaa aacgcaagtt gatcccatgc ggcttaataa
      181 aaacccgaaa tcccttgata acgatattcg cgctcgtctt gaaaataaac ctatgcttgc
      241 tccgcctgca atgcaggttt cagattcaga caacgccact acagcaaaga cgaatgatgc
      301 ccgcctgaca atgattttgg gtaatttaac aggcattgcc gatcaggata ttgcaacacg
      361 attgcataat aaccttgaaa gcaccctgct tcgacacgaa atggctcata ataagtttcg
      421 tgaattaagc gacgcctacg cctcgtcatt agattctgcg caaaaagctg acgatatcat
      481 gcatcaggcc aacaataatt acaatgcggt cgataaaaag gtgcaatcgc tggagaaaaa
      541 agtcaataca ttgaatcaag agctgtcgca actgcaacct ggcgatccgc aatataataa
      601 agtactgacg caaaaaaatg cggctgaaaa aacactcacg ctgtcattac aaaaaaaatc
      661 gttagctgag aaatcgttaa atacagccat tatggatgct gatgctgcga tcggccaaag
      721 tatggaaatt tttgacgaaa ttcaacagca agaacagatt aataacttca ccaccaatat
      781 ttgcctgacc caagaaaacc agaaaaacag aaacgcgaca gccacattta tccttttgat
      841 tacctcagta atggaagtta ttggcgatac caattgcgac tctattaaaa atcaatctga
      901 ggtaatgaaa gaaattaacc atgttaggga aaataaactc aatgaaacag cgcgtagcta
      961 tactacatgg acaaaagttt taaacattat taatgaggtc gttactgtag ttacctttgc
     1021 cgtaagtgca gccttaattg tgattggtat tgtcgcagca gttccaagtg ctggggcaag
     1081 ctttgctgga gcgctggcac taattggagg aatcgcgggg gcagtagtat taggtgttga
     1141 tattatttgt caaatcactc tgggcaccac ggctaccggt tggattttag gaaaacttac
     1201 tgaaggtctt tcgggtatta tttcaaaata tgaccccaca ctactcgcaa tctgcgctct
     1261 tctggacgcc ttgggtgtcc aacaaacgac tattgattta gtacaagata tttactcatc
     1321 tgcagccgct tctattgtta tggtaacatt aatgattggt gcaacattaa tttgttcagt
     1381 agcaataagt gaacttgtaa aggcactctc aaaaactgcc gccgaagaaa tcacaaaaga
     1441 aataactgac agtataaaat ccaccgtgga atctatgatc aagtcagttt caaaaaaaat
     1501 catgtcagca ttaaatttcc tcggtgacaa aataaaagca tcatcacaat ttataatgat
     1561 atttgcaaaa ataactattc ttcttgaaaa agttgccgca ggcgtttgta caataattac
     1621 atcgtgcatg aatcaatacg ctgccagtaa gtctgcagat atcgaaattc tacaacaaga
     1681 tataagcaat ctatcaaaaa cgcgcgaaca aatgcttaca gtgatgcaaa ggatagataa
     1741 aaccgtcgaa caagaggtag ctcagatggt gcgtgtatta caacaccgaa ctgaagcttt
     1801 gaaatttgct tctcattcta tcgtataag
//
LOCUS       DAPGOF010000324         1827 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.332, whole genome shotgun sequence.
ACCESSION   DAPGOF010000324 DAPGOF010000000
VERSION     DAPGOF010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.332"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>1827
                     /locus_tag="RHV13_004707"
     CDS             <1..>1827
                     /locus_tag="RHV13_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HDV1244135.1"
                     /translation="TTYRELNKAAQRSITAPDRIGEGKTWIYQRDTMNEREDNKGVHQ
                     YGPAEHFTHIISDKPSPKDKYVAYAINIPDYELAADVYNINVTSPSGQQETFKILINP
                     EHLRQTLERKSLTAVQKSQCEIITPKKPGEAILHAFNATYQQIRENMSEFARCHYGYI
                     QIPPVTTFRADGPETPEEEKGYWFHAYQPEDLCTIHNPMGDLQDFIALVKDAKKFGID
                     IIPDYTFNFMGIGGSGKNDLDYPSADIRAKISKDIEGGIPGYWQGQVLIPFTIDPVTK
                     ERKQIHPEDIHLTAKDFEASKDNISKDEWENLHALKEKRLNGMPKTTPKSDQVIMLQN
                     QYVREMRKYGVRGLRYDAAKHSKHEQIERSITPPLKNYNERLHNTNLFNPKYHEKAVM
                     NYMEYLVTCQLDEEQMSSLLYERDDLSAIDFSLLMKTIKAFSFGGDLQTLASKPGSTI
                     SSIPSERRILININHDFPNNGNLFNDFLFNHQQDEQLAMAYMAALPFSRPLVYWDGQV
                     LKSTTEIKNYDGSTRVGGEAWLNKGCSTYQQLYNEFHALYIDKAGIWSAFEGVFATKN
                     VLAFSRGDSVNINHTPHDGLVIINKGNEEVEGAWPNKLQPGK"
BASE COUNT          639 a          363 c          348 g          477 t
ORIGIN      
        1 atacaactta ccgtgaactg aataaagctg cacagcgtag tattacagcg ccagacagga
       61 taggagaagg aaaaacatgg atttatcaaa gagatacaat gaatgaaaga gaagataaca
      121 agggggttca ccagtatggt cctgctgaac atttcaccca cattatatct gacaaaccct
      181 ccccaaaaga taaatatgtt gcatatgcta ttaacattcc tgactatgag ctggcagccg
      241 atgtatataa tattaacgtg acgtcacctt ccggacagca agaaacattt aaaatactaa
      301 tcaatccaga acatctacgg caaacacttg agcgtaaatc tcttactgct gttcagaaat
      361 cacaatgtga aatcatcacc cccaaaaaac ctggcgaagc gattcttcat gcttttaatg
      421 ccacctacca gcaaatcaga gaaaatatgt ctgaatttgc acgttgccat tatgggtata
      481 tacaaatccc tccagtgaca actttccgtg ccgacggacc agaaactccc gaagaagaaa
      541 aaggttactg gtttcacgct tatcagcccg aagatctttg taccatccac aatccaatgg
      601 gagatttgca ggattttatc gcattggtta aagatgctaa aaaatttggt atcgatatca
      661 ttcctgatta tacctttaac tttatgggaa tcgggggtag tggtaaaaat gacctggatt
      721 atccctctgc tgatatacga gcgaagatca gtaaagatat agaaggtggt atccctggct
      781 attggcaagg tcaggttttg attccattta ctatagatcc agtaacaaaa gaacgtaaac
      841 aaatccatcc agaagatata catctcactg caaaagactt cgaagcaagt aaagataata
      901 tttctaagga tgaatgggaa aacctccatg cattaaaaga aaagcgttta aatggaatgc
      961 ctaaaacaac ccccaaaagt gaccaggtta ttatgctgca aaatcaatac gttcgtgaaa
     1021 tgcgaaaata tggcgtacga ggtttacgtt acgatgcagc aaaacactca aaacatgaac
     1081 aaatagaaag atcaataacc ccaccgctta aaaattataa tgagcgatta cacaatacta
     1141 acttatttaa cccaaaatat catgaaaaag ccgttatgaa ttacatggaa tatctggtaa
     1201 cttgtcagtt ggatgaagaa caaatgtcat cgctgcttta tgaaagagat gatttaagcg
     1261 ccattgattt ttcattgctc atgaagacga taaaagcttt ttcatttggt ggcgatctcc
     1321 aaacccttgc atcaaaaccg ggttccacaa tctcaagcat cccatcagaa agacggatat
     1381 tgattaacat taaccacgat tttcctaata atggcaatct tttcaatgac tttctattta
     1441 accaccaaca agatgaacaa ttagcaatgg catatatggc cgctctcccg ttcagcaggc
     1501 ctttagttta ctgggatggc caagtattaa aatcaacgac tgaaattaaa aattatgatg
     1561 ggtcgacgcg tgtcggcggt gaggcgtggc ttaataaagg ttgctctacc tatcagcagc
     1621 tctacaatga attccacgca ttatatatag ataaagcagg aatatggagt gcatttgagg
     1681 gtgtatttgc aactaaaaac gttctggcct ttagtcgtgg ggattctgtg aacattaatc
     1741 acactcctca tgatggacta gttataataa ataaaggaaa cgaagaagtt gaaggtgcct
     1801 ggcctaacaa attgcaacct ggaaaat
//
LOCUS       DAPGOF010000325         1816 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.333, whole genome shotgun sequence.
ACCESSION   DAPGOF010000325 DAPGOF010000000
VERSION     DAPGOF010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.333"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            172..675
                     /locus_tag="RHV13_004708"
     CDS             172..675
                     /locus_tag="RHV13_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HDV1244136.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(754..1005)
                     /locus_tag="RHV13_004709"
     CDS             complement(754..1005)
                     /locus_tag="RHV13_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HDV1244137.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1120..1206)
                     /gene="azuC"
                     /locus_tag="RHV13_004710"
     CDS             complement(1120..1206)
                     /gene="azuC"
                     /locus_tag="RHV13_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HDV1244138.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1469..1792
                     /locus_tag="RHV13_004711"
     CDS             1469..1792
                     /locus_tag="RHV13_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HDV1244139.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHALPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
BASE COUNT          468 a          434 c          399 g          515 t
ORIGIN      
        1 tttaagcatt ttcttatacc cgttcagacg ttattcttat ttcagatcat cgtcagaatt
       61 gactccacga tcacatttcg gaccggcaga aaggaattat tctgcaaaca gtaattatgg
      121 tgttttgatt tatcttgcac ctctccactt ctggatataa ggatattagg tatggcaacc
      181 gctggaatgc ttctcaaact caactctcaa atgaaccgcg agttttacgc atccaatctc
      241 taccttcacc tgagtaactg gtgttctgaa cagagtctga acggcaccgc cactttcctt
      301 cgcgcccagg cacagagtaa tgtgacccaa atgatgcgca tgtttaactt tatgaagagt
      361 gtcggcgcta cccccatcgt taaagccatt gatgttcccg gtgaaaaact gaactctctg
      421 gaagaactgt tccaaaaaac gatggaagaa tacgagcaac gttctagtac gttggcacag
      481 ttagccgatg aagcgaaaga actgaatgat gattcaaccg tcaatttcct gcgcgatctg
      541 gaaaaagaac agcagcatga tggtctgttg ctgcaaacca ttcttgatga agtgcgcagt
      601 gcgaaacttg cgggtatgtg ccctgtgcag accgaccaac atgttctgaa tgtcgtgtca
      661 caccagctgc attgatcata catcggcgct aatgcattgc gccgatgtag tttttgagaa
      721 accgctgcct catctttttg aagcagcggt tttttaatgg gattcgccct gtggggtaaa
      781 cttgagttca atcagcgcga tggctttttg gattgcccgc atggtgaccg ggtctgcggc
      841 ggcgggatgg ttagtaaagt cgatattctt cagctgactg gacatttttt cacgaacttc
      901 aacgggcgcg attacatcga gaacatccag aatttgtttg ataaccaact ggcaagcaac
      961 cacatcagaa accagttcct gatcggcatt cagcggctgg gacatcgtaa actcctgata
     1021 gcattttgaa agccgttata gtagcggctt cacatcttca acgatagtca catccaccgt
     1081 catcaggaca caaaaaaacc tgccggagca ggttttttgt tatcggaaca tattgcctgg
     1141 cggtacgtct ttgaacgtct tgcaatagtt attgaacata cttttcagga ttttgcgcag
     1201 tttcatcgcg gcactccgac catttgttat acaggtgtta ttgtctttgc gcttataata
     1261 tgacaaccat cacaaaaatc aatctttatg tgatacaaat cacataaata cccctttaat
     1321 gttataaaaa tgataatcaa aaaacagccc ccctatttct gacacctaca gatggcaaga
     1381 aatagcgcct gccaggcgtc ttttccggcc attgtcgcag cgctgtaacg cgtaaaatag
     1441 tgctttctct tactcttctg gctggaccat gagacttctg attctgactc tttcattaat
     1501 aacgctggcg gggtgtacag ttactcgcca ggcccatgtg agcgaagttg acgcggcaac
     1561 aggtattgta cggttggttt atgaccaggc ttttttgcag catgcccata ccgatcgtta
     1621 tgtcagtcgc gggatcgccg atcgcgcatg ccagcaggaa ggctataccc acgcgctccc
     1681 ctttggtcag ccagtaggca actgtagcct ctttgcgggt tccttatgtt tgaatacgga
     1741 attcactttg tcatatcagt gccatcattc cgccttccct gtctttctct aaatatttta
     1801 atcagcgagg gagatc
//
LOCUS       DAPGOF010000326         1806 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.334, whole genome shotgun sequence.
ACCESSION   DAPGOF010000326 DAPGOF010000000
VERSION     DAPGOF010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.334"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..871)
                     /locus_tag="RHV13_004712"
     CDS             complement(<1..871)
                     /locus_tag="RHV13_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage abortive infection AbiH family
                     protein"
                     /protein_id="HDV1244140.1"
                     /translation="MLVFLYNSTKGKTMRLYIIGNGFDMRHGLPTGYKHFKSYVEKND
                     QDLYDAIEEYIPAGDEWNELENALGEIDYELILQNSEIFLASYNTDDWSDAYHHDYQY
                     EVNKITQMLSARLKEQFADWVKGINIADAYNPKQYIPPIPRESIYFSFNYTNTLQQIY
                     AVPDEQIIHIHGNCSYDDDLILGHSFRVEKSLNPYIGPDQDTRIAEAYDRINEYFGNT
                     FKPSEDIIKEENVFFSSLKNVDEVIVLGHSLAEVDGEYFTEINKSIQENARWIVALYR
                     GEKKSGSLEDYGIR"
     gene            1448..1603
                     /gene="hokD"
                     /locus_tag="RHV13_004713"
     CDS             1448..1603
                     /gene="hokD"
                     /locus_tag="RHV13_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HDV1244141.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
BASE COUNT          494 a          383 c          368 g          561 t
ORIGIN      
        1 ctctgatacc ataatcttct aaactgcctg attttttctc acctctatat aaagcaacta
       61 tccatcgagc attttcttgg atgcttttat ttatttcagt aaagtattca ccgtcaactt
      121 cggcgagcga gtggcctaaa acgatgacct cgtccacatt tttaagcgat gagaaaaaaa
      181 cgttttcttc tttaattatg tcttcgctgg gcttgaatgt attaccgaag tattcgttta
      241 ttctgtcgta tgcctctgct attcttgtat cttgatccgg tcctatatat ggatttagtg
      301 acttttccac cctaaaacta tgaccaagta tgagatcgtc atcgtaacta caattgccgt
      361 gaatatgtat gatttgctcg tctggaacgg catagatctg ctgcaaggta tttgtataat
      421 taaatgaaaa atagatgctt tctcttggta tgggcgggat gtattgctta ggattgtaag
      481 catcggctat gttaatccct ttaacccaat cagcgaattg ctccttcaaa cgagctgaca
      541 gcatttgtgt gattttattt acttcatatt ggtaatcgtg atgataagca tcgctccaat
      601 catcggtatt gtatgaagcc aaaaatatct cactgttttg aaggataagc tcataatcaa
      661 tttcacctaa agcattttct aactcattcc actcgtcccc agcaggaatg tattcttcta
      721 tcgcgtcata tagatcttgg tcattttttt ctacatacga cttaaagtgc ttgtatcctg
      781 ttggtaagcc atgacgcatg tcaaatccat ttcctattat gtaaagcctc attgtttttc
      841 ccttagtcga attgtataaa aatactaaca tggcctgctc cacgttgact actatcccct
      901 cctttgagta atgccttcat cagatgtaac aacatttgcg agcttccaca gttcgctcaa
      961 agcggactag aaggttagct tgcgtcggac ttggcgtatt taaagaagtg ctggtggaga
     1021 ctggttgttg tgttccattt ctacagaaca aaatcacaga aactataccc aatagttata
     1081 ttgaatcaat gatgagacag cctcatattt atcaggactg gtgtacgtcc aatacaggag
     1141 gttgtcgtgc tggttctcaa atgtgcgcta gctattgcgg ctgtaatggc aatttattgt
     1201 cttgctgttg ttcttacgga tcgcctttct gattgatttt atattggcga ggtgacgtga
     1261 gttaagtaga attgctgcgg gtgcttgagg ctatctgcct caggcatgaa caccaaaggc
     1321 agatagagaa aagccccagt taacattacg cgtcctgcaa gacgcttaac attaatctga
     1381 ggccaatttc atgctagaca catgtaggtt agcctcttac gtgccgaaag gcaaggagaa
     1441 gcaggctatg aagcagcaaa aggcgatgtt aatcgccctg atcgtcatct gtttaaccgt
     1501 catagtgacg gcactggtaa cgaggaaaga cctctgcgag gtacgaatcc gaaccggcca
     1561 gacggaggtc gctgtcttca cagcttacga acctgaggag taagagacca ggcgagggag
     1621 aaatccctcg ccacctctga tgtgtcaggc atcctcaatg cacccgcact taacccgctt
     1681 cggcgggttt attttatctg taaatatttt tataaaaata atgcccacac acagcataaa
     1741 acaaaaagta tcacagataa aaaaggagcg taatgtgcag atttgttgtt ttccatattt
     1801 actcac
//
LOCUS       DAPGOF010000327         1789 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.335, whole genome shotgun sequence.
ACCESSION   DAPGOF010000327 DAPGOF010000000
VERSION     DAPGOF010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.335"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            380..1252
                     /locus_tag="RHV13_004714"
     CDS             380..1252
                     /locus_tag="RHV13_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1244142.1"
                     /translation="MSKNKARSKALHQTFSEIIPEMDKALNKQLLEVLMKYTERDNEL
                     IVILNEDGPNIIELKSLKPVSLLAEKLSAYSSYYHVDVVELVVKKIDFEGAYKLLKAS
                     PDVPLFKSLTELDKYLVEEFEKYGLNSFLDVDNLDYSLEKASELKNEQLINWVSDIIC
                     KREKLTLRKRFDVAVKAHYENVEKMYDTIRPLMKKLGFPEDLMTHTFSELSVFETKGW
                     DHAIKSKIETLAKRETQYLDDAAKAENRRLVTEKLENSLAIAPTKPTRNWLHIAGIAC
                     LVVCTFMYVTNKFI"
     gene            1310..1687
                     /locus_tag="RHV13_004715"
     CDS             1310..1687
                     /locus_tag="RHV13_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1244143.1"
                     /translation="MSYLEYINEVKVSGIVISAVEKKMTDQSIGLLIKLKNRMKTEVD
                     GEITEREFSVQIKVSPEMYSTCFTGINQGDELMVSGYIVVDTIMIEGREHPLDYMRVV
                     ATSKLAHIPKPLKGFGQSSFNQI"
BASE COUNT          588 a          312 c          368 g          521 t
ORIGIN      
        1 tacttatgct taaagctata gtataaaaac aatgagctta aatatcagct cccaaatttg
       61 agtaactttg cttaaagtta ctttcgcgtt aagcgttgat ttagccgtga aaagcggcca
      121 gcttaaacaa gctgtaagac accaccggca aaatgtcacc tttgtcgctg gtgcacttcc
      181 catgcctgaa aaaggcaatc tgaatttacc aaaccgtaac taatttagct cctcctggag
      241 ctatttttgt tgcgtacttc taatgccaga tagtataaat caggcggttc atagcttacg
      301 ggagatgaat gacaggcttt gccttcgtgt tgaaacaaaa aattgtttct tccttcttac
      361 tatatatagg tgaccccaaa tgtctaaaaa taaagctcgc agtaaagccc ttcatcaaac
      421 ctttagtgaa attattccag agatggataa ggcgctaaac aaacagctct tagaagttct
      481 gatgaaatat acagaacgtg ataatgaact gattgttatt ttgaatgagg acggcccaaa
      541 tatcattgaa cttaagtctc ttaagcctgt gtctttgttg gccgaaaagc tttctgctta
      601 ttcaagctat tatcatgtgg atgttgtgga gctcgtggtc aagaaaattg atttcgaagg
      661 agcttataag cttcttaaag cttccccaga tgtaccactt tttaaaagct taactgaact
      721 ggataaatat cttgttgagg agtttgaaaa atacggatta aattcatttc ttgacgtgga
      781 taatctggat tactcacttg aaaaagccag tgaactcaaa aatgagcagt taataaattg
      841 ggtttcggac atcatttgca aacgtgaaaa attaacttta cgtaagcgtt ttgatgtcgc
      901 agtaaaggcc cactacgaaa atgtagaaaa aatgtatgat accattcgtc cgttaatgaa
      961 aaaactgggt ttcccagaag atctcatgac gcacaccttt agtgagttaa gcgtttttga
     1021 aactaaagga tgggaccatg caataaaatc gaaaattgag actctcgcta agcgtgaaac
     1081 tcaatattta gatgatgcgg ctaaagcaga gaatcgacgt cttgtaacag agaagttgga
     1141 gaactcttta gcgattgcac caactaaacc aacaagaaat tggttacata ttgccggaat
     1201 tgcctgtttg gtagtctgca cgttcatgta tgtaactaat aaatttattt aatcctgtta
     1261 agggggaacc ctaacggggc ttctctttga acaaatcgag gagcggaaaa tgtcatatct
     1321 tgaatatatc aatgaagtaa aagtgtccgg gatagttatc agtgctgtgg agaaaaaaat
     1381 gactgaccag agtataggtt tgttaataaa gctcaaaaat aggatgaaga cagaagttga
     1441 tggtgaaatc actgagagag aattttcagt tcaaatcaag gtgtcgcctg aaatgtattc
     1501 aacctgtttc actggtataa atcagggtga tgagttgatg gtctccggtt atattgttgt
     1561 cgatactatt atgattgaag ggagagaaca tcctcttgac tacatgagag tggtagcaac
     1621 aagtaagttg gctcacatac ctaaacctct aaaaggtttt ggacaaagta gctttaatca
     1681 gatctgacat taaacccacc ctaaagggga gaaatccctt catgggattt ctccttttgg
     1741 agtccccatg aaacaaaaat ttagggctac tgcaataagt agtaatcaa
//
LOCUS       DAPGOF010000328         1787 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.336, whole genome shotgun sequence.
ACCESSION   DAPGOF010000328 DAPGOF010000000
VERSION     DAPGOF010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.336"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>1787
                     /locus_tag="RHV13_004716"
     CDS             <1..>1787
                     /locus_tag="RHV13_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDV1244144.1"
                     /translation="RGELAAVYDRSNTQVRSFTYDDKYRGRMVAHRHTGRPEIRYRYD
                     RDGRVTEQLNPAGLSYTYQYEKDRITITDSLDRREVLHTQGEAGLKRVVKKEHADGSV
                     TQSQFDAVGRLKAQTDAAGRTTEYSPDVVTGLITRITTPDGRASAFYYNHHSQLTSAT
                     GPDGLEIRREYDELGRLIQETAHNGDITRYRYDNPHSDLPCATDDATGSRKTMTWSRY
                     GQLLSFTDCSGYVTRYDHDRFGQMTAVHREEGLSQYRAYDSRGQLIAVKDMQGHEMRY
                     EYNIAGDLTAVIAPDGSRNGTQYDAWGKAIRTTQGGLTRSMEYNAAGRVIRLTSENGS
                     HTTFRYDVLDRLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTHWHYDEADRLTQRTV
                     KGETAERWRYDERGWLTDISHISEGHRVTVHYGYDEKGRLTGERQTVHHPQTEALLWQ
                     HETRHAYNAQGLANRCIPDSLPAVEWLAYGSGYLAGMKLGDTPLVDFTRDRLHRETLR
                     SFGRYELTTAYTPAGQLQRQHLNSLQYDRDYTWNDNGELIRISSPRHTRSYSYSTTGR
                     LTGVHTTTSTLDIRIPYATDPAGNRLPDP"
BASE COUNT          435 a          547 c          536 g          269 t
ORIGIN      
        1 cgcggcgaac tggcggcggt gtatgaccgc agcaatacac aggtgcgcag ctttacttac
       61 gatgataaat accggggccg gatggtggcg caccgtcaca cgggccgacc ggaaatccgt
      121 taccgttacg accgcgacgg gcgggtgaca gaacagctaa acccggcagg cttaagctac
      181 acgtatcagt atgagaaaga ccgcatcacc atcaccgaca gcctggaccg ccgtgaagtc
      241 ctgcacacgc agggtgaagc cgggctgaag cgggtggtga aaaaggaaca cgcggacggc
      301 agcgtcacgc agagtcagtt tgacgcggtg ggcaggctta aggcacagac ggatgccgca
      361 ggccggacga ctgagtacag cccggatgtg gtgacgggac tcatcacgcg catcaccacg
      421 ccggatggca gggcatcggc gttttactat aaccaccaca gccagttaac gtcagccacc
      481 gggccagacg ggctggaaat acgccgggaa tatgatgaat tgggccgtct gattcaggaa
      541 actgcacaca acggcgatat cacccgctac cgttatgata atccacacag tgacttaccc
      601 tgcgcaacgg acgatgccac cggcagccgg aaaaccatga catggagccg ttacggccag
      661 ttgctgagct tcaccgactg ttccggttat gtaacccgtt atgaccatga ccgcttcggg
      721 cagatgacgg cggtgcaccg cgaggaaggg ctgagccagt accgtgcata cgacagccgt
      781 ggacagttaa ttgcggtgaa agacatgcag ggccatgaaa tgcggtatga atacaacatc
      841 gccggtgacc tgaccgccgt cattgccccg gacggcagca gaaacgggac acagtacgat
      901 gcgtggggaa aggccatccg taccacgcag ggcggtctga cgcgcagtat ggaatacaat
      961 gctgccggac gggtcatccg cctgaccagt gaaaacggca gccacaccac cttccgttac
     1021 gatgtactcg accggctgat acaggaaacc ggctttgacg gccgcacaca gcgttatcac
     1081 cacgacctga ccggcaaact tatccgcagc gaggatgagg ggctggtcac ccactggcac
     1141 tatgacgaag cagaccgcct cacgcagcgc accgtgaagg gtgaaaccgc agagcggtgg
     1201 cggtacgacg aacgcggctg gctgacagac atcagccata tcagcgaagg gcaccgggtg
     1261 acggtgcatt acgggtatga tgagaaaggc cggctgaccg gtgagcgtca gacggtgcat
     1321 cacccgcaga cggaagcact gctctggcag catgagacca gacatgcgta caacgcgcag
     1381 ggactggcga accgctgtat accggacagc ctgcccgccg tggaatggct ggcctacggc
     1441 agcggttacc tggcaggcat gaagctcggc gacacaccgc tggtggattt cacccgcgac
     1501 cgcctgcacc gggaaacgct gcgcagcttc ggccgttatg aactcaccac cgcttacacc
     1561 cctgccgggc agttacagcg tcagcacctg aacagcctgc agtatgaccg cgattacacc
     1621 tggaacgaca acggcgaact catccgcatc agcagcccgc gccatacccg gagttacagc
     1681 tacagcacca ccggcaggct gaccggcgtt cacaccacca cctcaactct ggatatccgc
     1741 atcccgtatg ccacagaccc ggcaggtaac cgcctgcccg acccgga
//
LOCUS       DAPGOF010000329         1741 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.337, whole genome shotgun sequence.
ACCESSION   DAPGOF010000329 DAPGOF010000000
VERSION     DAPGOF010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.337"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(330..539)
                     /locus_tag="RHV13_004717"
     CDS             complement(330..539)
                     /locus_tag="RHV13_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin"
                     /protein_id="HDV1244145.1"
                     /translation="MWRALGGKLKVTQLSSTGYLGQFDFCAIARMGNAEDSHYCQVVE
                     SPSGSRKWYKYEHKTGCIASCVTLN"
     gene            614..1738
                     /locus_tag="RHV13_004718"
     CDS             614..1738
                     /locus_tag="RHV13_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000486721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDV1244146.1"
                     /translation="MFQKIKIRKMTISRALDKYLKTVSIHKKGHLQEFYRVNVIKRHP
                     IAERYMDDITTVDIANYRDQRLAQINPRTGRQITGNTVRLELALLSSLFNIARVEWGT
                     CRMNPVELVRKPKISSGRDRRLTSGEERRLSRYFKEKNQALYVIFHLALETAMRQGEI
                     LSLRWEHVDLQHGVAHLPTTKNGAPRDVPLSRKARNYLQMLPTQLNGNIFSYTSSGFK
                     SAWRTALQELKIENLHFHDLRHEAISRFFELGTLNVIEVAAISGHRSLNMLKRYTHLR
                     AYQLVSKLDARRKQTSKIAPYFVPYPATVENRNGQVVVTLSDFDLETSAATKEQAIFH
                     ASVLLLRTLAQAAQRGERVPTPGELPTNIDERVMICPLTN"
BASE COUNT          481 a          434 c          387 g          439 t
ORIGIN      
        1 acttccggtt cctctaacgg tagttacagt aatcttggat cacaccgagg atcatttact
       61 ggtcgcaata cgggaagtgg tacgctgttt gtttacgcct ctggagggaa tggtggttcc
      121 gccgggggag attgtgctaa cacatccaga ttgcagggat acgttgcagg ggcattgatt
      181 agcacaaatg cgagtaataa cccatcttat gggaagacag cattcatctc ttttgctgtc
      241 cctgctggcg caacttatca gatcacctct tatccggccc aaaactactc ttgtggctca
      301 ggggtatttt cagtatttgg ataccaaact taattgagag ttacacagga cgcaatacaa
      361 cctgtcttgt gctcgtattt gtaccatttt cgtgaaccag atggactctc gactacctgg
      421 cagtaatgag aatcctcagc atttcccatt ctggcaatgg cacaaaagtc aaattgcccc
      481 aagtaaccag tgctggaaag ttgagtaacc ttgagctttc cacctaatgc cctccacaca
      541 ccggattggc acggtgatat tgcatttcga agcaagaatc tctaccttcc cccctttttt
      601 gtctggaggg gatatgtttc agaaaatcaa aattcgcaaa atgacaatca gccgggcgct
      661 ggacaaatac ctgaagacag tatccatcca caaaaaaggc catctacagg agttttatcg
      721 cgtaaatgtg attaaacgtc accctattgc tgaacgctat atggatgaca ttaccactgt
      781 tgatattgcc aattatcggg atcaacggtt ggcgcaaatt aacccacgaa cggggcgtca
      841 aataaccgga aacaccgtcc gtcttgaact tgccctgttg tcatcattat tcaatatcgc
      901 ccgtgtggaa tggggaacat gccgcatgaa tcctgtcgaa ctggtcagaa aacccaaaat
      961 cagtagcggg cgcgatcgtc gcctgacttc aggagaagaa cgccgacttt cgcggtattt
     1021 caaagaaaaa aatcaggctc tttacgtcat ttttcatctg gcgctggaaa cagcaatgcg
     1081 acaaggtgaa atcttgtcat tgcgttggga acacgtcgat ctgcaacatg gggtagctca
     1141 tcttccaaca accaaaaacg gtgctccacg cgatgttccg ctatccagga aagcccgtaa
     1201 ttacctgcaa atgctgccga cacagcttaa cggtaatatt ttttcttata cctcttcggg
     1261 attcaaaagt gcctggcgaa cagcacttca ggaactgaag atagaaaact tgcattttca
     1321 tgacctccgt catgaggcta tcagccgatt tttcgaactt ggtacattga acgtcattga
     1381 ggttgccgct atttcaggcc atcgctccct gaacatgttg aaacgataca cacaccttcg
     1441 ggcttatcaa cttgtcagta aactcgatgc ccgacgcaag caaaccagca aaatcgctcc
     1501 ctattttgtt ccctacccgg caacggtcga gaacagaaac gggcaagtcg ttgtgactct
     1561 gagtgatttc gacctggaaa caagcgcagc cacaaaagaa caggccatat ttcatgccag
     1621 cgtgttgctg ctacgaacac tggcacaagc cgcccagcga ggagaacgcg tccctactcc
     1681 tggcgaactt cctacaaaca ttgatgaacg ggtaatgatt tgtccattaa caaattagag
     1741 g
//
LOCUS       DAPGOF010000330         1689 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.338, whole genome shotgun sequence.
ACCESSION   DAPGOF010000330 DAPGOF010000000
VERSION     DAPGOF010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.338"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            68..1210
                     /locus_tag="RHV13_004719"
     CDS             68..1210
                     /locus_tag="RHV13_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDV1244147.1"
                     /translation="MTNKHPSLFSPFMLTEKIKLRNRIVMAPMTTWSANPDGTISEQE
                     LEFYKRRSQNVGLVITGCTYVTPSGIGFTHEFAAYDDRFINSLEKLAAAAKSGGAPAI
                     LQIFHAGNKAIPELVPNNDVISASASSVKSGDFMKRVVQSREMTENEIQETIRAFGDV
                     TKRAIKAGFDGIELHGAHGFLLQNFFSPLFNQRNDRWGGDLEGRMRFPLAVLQEVKNV
                     VYEYATKPFAIGYRISPEESATGGLRIEDTYKLLDRLISSGISYIHTSLVSINDSYPV
                     ESPNGPRTIELILNHIAGRVPVIAAGKIRTPSQAQEAISTGLPLVAIGKGLVINPEWV
                     TLAESGRGHEIQTTLNPQLVPELTIPDKLWDQIQASKGTGWFPLMD"
BASE COUNT          519 a          367 c          362 g          441 t
ORIGIN      
        1 aaagctggca acaactaacc agttaaaaca tacgcatcaa aatcagataa acatttgagg
       61 tattgttatg acaaataaac acccatcatt gttttctcct tttatgctga cagaaaaaat
      121 aaagctacgt aatcgtatcg ttatggctcc tatgacgacc tggtcagcga atcctgatgg
      181 aaccatctct gagcaagaac tcgaatttta taaacgccgc tcacaaaatg ttggtttagt
      241 aataacagga tgtacctatg tcacgccaag cggcattggg tttactcatg aatttgccgc
      301 gtatgacgac cgtttcatta acagccttga aaagttagca gcggcagcca aaagtggggg
      361 tgctccggct attttgcaga ttttccacgc cggtaataaa gccatcccgg agcttgtacc
      421 aaataacgat gttattagtg ccagcgcatc aagcgtaaaa tctggtgact ttatgaagag
      481 agtggtacaa agcagggaaa tgactgaaaa cgaaattcag gaaaccatta gagcattcgg
      541 agatgtcaca aaaagggcga ttaaagcagg ttttgatggc attgaacttc atggtgcgca
      601 cggattcctt ctacagaatt tcttctcccc tctatttaac cagcgtaatg accgatgggg
      661 aggagatctc gaaggacgta tgcgcttccc gctggccgta ttacaggaag taaaaaatgt
      721 agtctatgaa tatgcgacaa agccctttgc catcggttac agaatatctc ctgaagaatc
      781 tgcaacaggg gggctgcgga tagaggatac ttataaactc cttgatcggc tcatttcatc
      841 cggtatttcg tatatacata cctcgctggt cagtattaac gatagctacc ctgttgagtc
      901 gccaaatggc ccccgaacta ttgaattgat tttaaatcat attgcaggca gagtgccggt
      961 aatcgctgca ggtaaaatac gcacaccgtc tcaggctcaa gaagcaattt ccactggcct
     1021 gccacttgtt gcgatcggta aagggctggt gataaatcca gaatgggtga ctttagctga
     1081 atcaggtaga ggccatgaaa ttcaaacaac ccttaatcca caacttgttc ctgaattaac
     1141 cattccggat aaactatggg atcaaattca ggcatcgaaa ggaactggct ggtttccgtt
     1201 aatggattaa atatctatta ctctcccgag agtaaaacta tcaaatcaat gagtaaccga
     1261 tacagcctca caaattcttt cagataatta acccgtcact gcggcacgtt ctctcataag
     1321 gcgtgcctgc agtgtaatcc agtatgtatc cggcatagtt gacggtttaa tagagctcaa
     1381 ttcctcgccc aggttaccaa caacgatatc ttgtataaca gaccaggaga tacgacatta
     1441 tgatgagatg cgatatgctc ctcagcgcca atcaacgccc ggactgggcc agtcttgaga
     1501 tcagactcat tcccggaact gacttcaggc gacatatatc aatgacgttg gtgcaataaa
     1561 agatagccga tgtaatacat ctattcaaat ttgtgataca tgtcaaaaaa ttggttgacc
     1621 aaactcgtta tttatataag ggcacttacg aagtgcgctc ttttttaaag cgaggaagta
     1681 ccaatgaaa
//
LOCUS       DAPGOF010000331         1651 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.339, whole genome shotgun sequence.
ACCESSION   DAPGOF010000331 DAPGOF010000000
VERSION     DAPGOF010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.339"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            29..136
                     /locus_tag="RHV13_004720"
     CDS             29..136
                     /locus_tag="RHV13_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1244148.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            342..1625
                     /gene="gltA"
                     /locus_tag="RHV13_004721"
     CDS             342..1625
                     /gene="gltA"
                     /locus_tag="RHV13_004721"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HDV1244149.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
BASE COUNT          426 a          404 c          391 g          430 t
ORIGIN      
        1 ttacaaacat taccaggaaa agcatataat gcgtaaaagt tatgaagtcg gtatttcacc
       61 taagattaac ttatgtaaca gtgtggaagt attgaccaat tcattcggga cagttattag
      121 tggtagacaa gtttaataat tcggattgct aagtacttga ttcgccattt attcgtcatc
      181 aatggatcct ttacctgcaa gcgcccagag ctctgtaccc aggttttccc ctctttcaca
      241 gagcggcgag ccaaataaca aacgggtaaa gccaggttga tgtgcgaagg caaatttaag
      301 ttccggcagt cttacgcaat aaggcgctaa ggagacctta aatggctgat acaaaagcaa
      361 aactcaccct caacggggac acagctgttg aactggatgt gctgaaaggc acgctgggtc
      421 aagatgttat tgatatccgt actctcggtt caaaaggtgt gttcaccttt gacccaggct
      481 tcacttcaac cgcatcctgc gaatctaaaa ttacttttat tgatggtgat gaaggtattt
      541 tgctgcaccg cggtttcccg atcgatcagc tggcgaccga ttctaactac ctggaagttt
      601 gttacatcct gctgaatggt gaaaaaccga ctcaggaaca gtatgacgaa tttaaaacta
      661 cggtgacccg tcataccatg atccacgagc agattacccg tctgttccac gctttccgtc
      721 gcgactcaca tccaatggca gtcatgtgtg gtattaccgg cgcgctggcg gcgttctatc
      781 acgactcgct ggatgttaac aatcctcgtc atcgtgaaat tgccgcgttc cgcctgctgt
      841 cgaaaatgcc gaccatggcc gcgatgtgtt acaagtattc cattggtcag ccatttgttt
      901 atccgcgcaa cgatctctcc tatgccggta acttcctgaa tatgatgttc tccacgccgt
      961 gcgaaccgta tgaagttaat ccgattctgg aacgtgctat ggaccgtatt ctgatcctgc
     1021 acgctgacca tgaacagaac gcctctacct ccaccgtgcg taccgctggc tcttcgggtg
     1081 cgaacccgtt tgcctgtatc gcagcaggta ttgcttcact gtggggacct gcgcacggtg
     1141 gtgctaacga agcggcgctg aaaatgctgg aagaaattag ctccgttaaa cacattccgg
     1201 aatttgttcg tcgtgcgaaa gataaaaatg attctttccg cctgatgggc ttcggtcacc
     1261 gcgtgtacaa aaattacgac ccgcgcgcca ccgtaatgcg tgaaacctgc catgaagttc
     1321 tgaaagagct gggcaccaaa gatgacctgc tggaagtggc tatggagctg gaaaacatcg
     1381 cgctgaacga cccgtacttt atcgagaaga aactgtaccc gaacgtcgat ttctactctg
     1441 gtatcatcct gaaagcgatg ggtattccgt cttccatgtt caccgtcatc ttcgctatgg
     1501 cccgtaccgt tggctggatc gctcactgga gcgaaatgca cagtgacggc atgaagatcg
     1561 cccgtccgcg ccagttgtat acaggatatg aaaaacgcga ctttaaaagc gatatcaagc
     1621 gttaatggtt gattgctaag ttgtaaatat t
//
LOCUS       DAPGOF010000332         1629 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.340, whole genome shotgun sequence.
ACCESSION   DAPGOF010000332 DAPGOF010000000
VERSION     DAPGOF010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.340"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            442..1185
                     /locus_tag="RHV13_004722"
     CDS             442..1185
                     /locus_tag="RHV13_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDV1244150.1"
                     /translation="MPLVLISTEGATEWCAEYDEWGNLLNEENPHQLQQLIRLPGQQY
                     DEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNFYQYPLNPVQYIDSMGLASKYGHL
                     NNGGYGARPNKPPTPDPSKLPDIAKQLRLPYPIDQASSAPNVFKTFFRVLSPYDYTLY
                     CRKWVKPNLTCTPQDDPQYPGMDTKTASDYLPQTNWPTTQLPPGYTCAEPYLFPDINK
                     PDGPATAGIDDLGEILAKMKQRTSRGIRK"
     gene            1182..>1629
                     /locus_tag="RHV13_004723"
     CDS             1182..>1629
                     /locus_tag="RHV13_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1244151.1"
                     /translation="MKRVLFFLLMIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFP
                     VLNNKAMWRWYQNEDIGEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHTIKGT
                     LQELINTAEKTAFLGDRFRSYIRAGIYQKKSSDPAQLLAVLDNSIMV"
BASE COUNT          471 a          368 c          429 g          361 t
ORIGIN      
        1 tggcgacgtg aacgggacct gacgggctgg atgtcgctgt cacggaaacc gcaagtgacc
       61 tggtacggct gggacggcga ccggctgacc acaatacaga acgacagaac ccgcatccag
      121 acgatttatc agccggggag cttcacgcca ctcatcaggg ttgaaaccgc caccggtgag
      181 ctggcgaaaa cgcagcgccg cagcctggcg gatgcgcttc agcagtccgg cggcgaagac
      241 ggtggcagtg tggtgttccc gccggtgctg gtgcagatgc tcgaccggct ggaaagtgaa
      301 atcctggctg accgggtgag tgaggaaagc cgccgctggc tggcatcgtg cggcctgacg
      361 gtgaagcaga tgcaaaacca gctggacccg gtgtacacgc cggcgcgaaa aattcacctg
      421 taccactgcg accatcgcgg cctgccgctg gtgcttatca gcacggaagg ggcaacagaa
      481 tggtgcgcag aatacgatga gtggggcaac ctgctgaatg aagagaaccc gcatcagctg
      541 cagcagctta tccgcctgcc ggggcagcag tatgatgagg agtccggcct gtattacaac
      601 cgccaccgct attatgaccc gctgcagggg cgatatatca ctcaggatcc gattggactg
      661 aaggggggat ggaattttta tcagtatccg ttgaatccgg ttcagtatat agattcaatg
      721 ggactggcat caaaatatgg acacttaaat aatggcggat atggagcgag acccaacaaa
      781 ccgcctacgc ccgatccaag taaattgcca gacatagcga aacaattaag actgccatat
      841 cctattgacc aggccagtag tgcgcctaat gttttcaaaa cattcttcag agtattaagc
      901 ccttacgact acacactgta ttgcaggaag tgggtaaaac caaatctgac ttgtacgcca
      961 caggatgatc cccagtatcc agggatggat acaaagacag caagtgatta cctgccacag
     1021 acaaattggc caacaactca attaccacca ggatatactt gtgcagaacc ctatttattc
     1081 ccagacatta ataaacccga tgggccagca acagcaggga tagatgattt gggtgaaatt
     1141 ttagctaaaa tgaaacagag aacatcgaga ggaataagaa aatgaaaaga gttttgttct
     1201 ttttgctgat gatatttgtt agttttggtg ttatagctga ttgcgaaata caagctaaag
     1261 atcatgattg ttttactatt ttcgctaagg gtactatttt ttccgcgttt cctgtcttaa
     1321 ataataaagc catgtggaga tggtatcaga atgaggacat cggtgagtat tattggcaaa
     1381 cagaactggg cacatgtaaa aataataaat tcacaccaag cggggcgaga ctcttaattc
     1441 gcgtcgggtc actacgcctt aatgaaaatc ataccatcaa aggaacgttg caggagttga
     1501 taaatacagc agaaaaaacg gcgtttctcg gcgatcggtt tagaagttat ataagagcgg
     1561 gtatctatca aaaaaagagc agtgatccag ctcagttgtt ggcagtcctt gataattcaa
     1621 tcatggtta
//
LOCUS       DAPGOF010000333         1619 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.341, whole genome shotgun sequence.
ACCESSION   DAPGOF010000333 DAPGOF010000000
VERSION     DAPGOF010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.341"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..>1619)
                     /locus_tag="RHV13_004724"
     CDS             complement(<1..>1619)
                     /locus_tag="RHV13_004724"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV1244152.1"
                     /translation="KRSLQVDLQSDNAIDFDGSSNALLGIKGILPITHGGLGASSVSG
                     GRTNLGLGVADIPQFKGINLVNSTDADLAASGIVSGYLNNSAGVQRSRFRIYSEIRSD
                     NRSWLTLHLQSDTNTNKYAGLDIDGNFLIAGDSKCRALEPTDVALTRKNIDVYSKAEV
                     DLKKGMKFTRVNAPSGAEEGKFYPVVIKRSATSNGELASRVIISTAPRQAAHRMNNCE
                     FNGFVMPAGWSDRGRYAYGMFWQYQDAERAIHSIAMSNKDDEVSSVFYIEGGAFPVCV
                     LVEEGLSVVVPTTDYIVGQTTYKWGATNPKAECIAADIIIDFSNGRGFYSSGNLNGNA
                     ATATKLQTARTINGVTFDGTTDISLTPENIGALSLSGGTLLGGLTAPLLTTKSDLIFS
                     SHTSRHIRFTYTKNDGTTLTDGYIFKDGVDNPNRRPGIRINCAAPNKETGNGTNSGDW
                     IFGEDGIFTMPGGTVLRGGINSNGVPQSEYKHDMLTSVVDDAKMYLRRFRSSTGASIW
                     HETIENNVYRLCTGTTDAQEELVLRTGSYAK"
BASE COUNT          429 a          386 c          327 g          477 t
ORIGIN      
        1 aatttcgcat aggaaccagt cctcaagact aactcttcct gagcgtcagt tgttcctgtg
       61 cataagcgat aaacgttatt ctctattgtt tcatgccata tgcttgcacc tgtgctggag
      121 cggaaacgtc gcagatacat ttttgcatcg tcaacaactg acgtgagcat gtcatgttta
      181 tactcacttt gcggcacgcc attgctattg attcctcccc gaagaacagt gccacccggc
      241 atggtaaaaa tgccatcttc accgaatatc caatcacctg aattagtacc attgccggtt
      301 tctttattgg gcgcagcaca atttatccgg atacctggtc gtctgttagg gttatcgaca
      361 ccatctttaa atatgtaccc atcagttagt gttgtgccgt catttttcgt atatgtaaag
      421 cggatatgac ggcttgtatg gctactaaaa ataagatcgc ttttagtggt aagtaaagga
      481 gctgtcagac ctcctaataa tgtgcctccg gatagtgaca gcgcgccaat gttctctggt
      541 gtcaacgaaa tatcagtagt gccatcgaag gttactccgt tgattgttct ggcggtctgt
      601 aatttagttg ctgttgcagc attaccattg aggtttccag agctatagaa accacgacca
      661 ttgctaaaat caataattat gtcggcagct atacattcag ctttagggtt ggttgccccc
      721 catttgtatg tggtttgtcc aacaatataa tccgttgtag ggacaacaac agaaagcccc
      781 tcctccacaa gaacgcatac tggaaatgct ccgccttcaa tataaaaaac tgagctaact
      841 tcatcgtctt tattgctcat cgcaatagaa tgaatcgccc gttctgcatc ctgatattgc
      901 cagaacatcc cgtatgcgta ccgccctctg tcactccaac cagcaggcat cacaaaacca
      961 ttgaattcgc agttgttcat gcgatgtgca gcttggcgcg gcgcagtgga aataataacc
     1021 cgagaagcaa gttcaccatt gctcgttgca gatctcttga tgactacagg atagaattta
     1081 ccctcttcag ctccagaagg agcattgact ctggtgaatt tcatcccttt tttaaggtct
     1141 acttcggctt tgctgtaaac atcaatattt tttcttgtta acgctacatc agttggttct
     1201 aatgctcggc atttgctatc accggcaatc agaaaattac cgtcaatgtc taatccggca
     1261 tatttattgg tgttggtatc agattgtaga tgtaacgtca gccatgagcg gttgtcactc
     1321 ctgatctctg aataaattct aaaacgactt ctctgtaccc cagctgaatt attcagataa
     1381 ccacttacaa tccctgacgc cgctaaatca gcgtctgttg agttaacaag gttgataccc
     1441 ttaaattggg ggatgtccgc aacacctaat ccaagattag tacgccctcc agagacgctg
     1501 gatgcaccta atcccccatg agtaattggt aaaatacctt ttatgcccag aagagcattt
     1561 gatgaaccat caaagtcgat ggcgttatca ctttggaggt caacttgaag cgacctttt
//
LOCUS       DAPGOF010000334         1605 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.342, whole genome shotgun sequence.
ACCESSION   DAPGOF010000334 DAPGOF010000000
VERSION     DAPGOF010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.342"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            226..926
                     /locus_tag="RHV13_004725"
                     /pseudo
     CDS             226..926
                     /locus_tag="RHV13_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(946..1011)
                     /locus_tag="RHV13_004726"
                     /pseudo
     CDS             complement(946..1011)
                     /locus_tag="RHV13_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease"
     gene            1244..1348
                     /locus_tag="RHV13_004727"
                     /pseudo
     CDS             1244..1348
                     /locus_tag="RHV13_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          449 a          348 c          374 g          434 t
ORIGIN      
        1 ggaaataaaa ttgtttccaa tagacatttt taacatgttg tttttctaag tgttataagg
       61 taggtataaa atgggatgga gcctctgctt ctggcatgtg tcggtcagaa tgactcatga
      121 tgtggtctgc tattattgac atcctcactg ccctaaagga tggggatttc ggtaatgctg
      181 ccaacttact gatttagtgt atgatggtga ttttaaggtg cttgcgtggc ttccatttcc
      241 atcagatgtc cttcctgctc cgctactgaa ggcgtggtgc gtaacggcaa aagcactgcc
      301 ggacatcagc gctatctctg ctctccttgc cgtaaaacat ggcaactaca gttcacttac
      361 accgcctctc agcccggtaa gcaccagaaa atcattgata tggccatgaa tggcgtcgga
      421 tgtcgcgcca gtgcacgcat cattatgggc gttggcctca acacggtttt acgtcactta
      481 aaaaactcag gccgcagtcg gtaacctcgc gcatacaacc gggcagtgat gtgattgtct
      541 gcgctgaaat ggacgaacag tggggctacg tcggtgctaa atcacgtcag cgctggctgt
      601 tttacgcgta tgacaggata cggaggacgg ttgtggcgca cgtcttcggt gaacgcactc
      661 tggccacact ggagcgtctt ctgagcctgc tgtcggcctt tgaggtcgtg gtatagatga
      721 cggatggctg gccgctgtat gaatcacgcc tgaagggaaa gctgcacgtt atcagcaagc
      781 gttacactca gcgcattgag cgacataatc tgaatctgag acaacatctg gcaaggctgg
      841 gacggaagtt actgtcgttc tcaaaatcgg tggagttgca tgacaaggtc atcgggcatt
      901 atctgaacat aaaacactat cagtaagttg gagtcattac ctcctcccga actcaaagct
      961 atcatacaga taattactgg ttaaccctat atagggcatt ttaaagtgtt gtgatcttac
     1021 ccagcaatag tggacacgcg gctaagtgag taaactctca gtcagaggtg actcacaaga
     1081 caaaacagtt tcaaccagta aaaaaacgta aacaccattc gcatgaattt cgtagttaag
     1141 atctgaagct tgcagaatgc atcggtatta ctgccggagt ccgtgaactt agtctgcatg
     1201 aatcaccaca gctttacatc tgtcgcagta aacagcaaaa tcagcaaact tacttaatta
     1261 tatcgagtgc gattacaatc gctggcgatg gtacagttgg tgtggcggtc tcagtccgga
     1321 acaatttgaa aatcagaatc tcacttaaaa cggcatccat tttacaaaag atggccgtct
     1381 gtgtatttgt aatgaatgcc ttctacatgt ttagagtgca taatccacga caaggcgata
     1441 tcctaaaaat tagctagcag gcaactttga tggcatagat aatgttttta gtaaaaaaga
     1501 gttgactgaa tataatacag cctcttccta cataagaagg aatgtgctca caaaaaaaaa
     1561 ccattagtct gcttcctgtt cttacaataa tcagtattat gtatt
//
LOCUS       DAPGOF010000335         1601 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.343, whole genome shotgun sequence.
ACCESSION   DAPGOF010000335 DAPGOF010000000
VERSION     DAPGOF010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.343"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..171
                     /locus_tag="RHV13_004728"
     CDS             <1..171
                     /locus_tag="RHV13_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1244153.1"
                     /translation="GATWYVAQHEKEASLRFYARAFLDMKKITEESMRKLPKSDQQNI
                     KLKLDEIDSRKP"
     gene            191..631
                     /locus_tag="RHV13_004729"
     CDS             191..631
                     /locus_tag="RHV13_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001687636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1244154.1"
                     /translation="MTTFTDDDKGLIKEIRERIGSLDVRDNIERRAYEIALASLEAGV
                     VAWRYRYVKKDVTDSQGKTWVGDWKYVPTKEDCNDRPNYEIQELYNLPPAIAAPTRGL
                     VNAVRFYDQVKHTNPPVETGAWKDAIDWVLKEACQAVNIDAKGE"
     gene            633..959
                     /locus_tag="RHV13_004730"
     CDS             633..959
                     /locus_tag="RHV13_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1244155.1"
                     /translation="MTTFTDDDKKLIKEIRERIGSLDVRDNIERRAYEIALASLEAEA
                     PNLPGGFTIEEAKELHENLVKSHVSKALSGEKMKKENRDADLRWIHGVIVQAAWFVKA
                     SLGQNA"
     gene            complement(1219..1518)
                     /locus_tag="RHV13_004731"
     CDS             complement(1219..1518)
                     /locus_tag="RHV13_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDV1244156.1"
                     /translation="MFFSVGVETPKDDQTAYGITVPAFDCFGFGCVSAADSQAEIPAM
                     AREAILAIVEDMVLSGAYSVDDIHDEGCLTYSDHSNYKHCDSWFLVDVDLTLDRP"
BASE COUNT          542 a          352 c          364 g          343 t
ORIGIN      
        1 ggcgcaacct ggtatgttgc acaacatgaa aaagaggcaa gcctgcgctt ctatgcaaga
       61 gcttttcttg atatgaaaaa aataactgaa gagagcatga gaaaattacc aaaatcagac
      121 cagcaaaata taaaactcaa actagacgaa attgattcaa gaaaaccata gatatgaaaa
      181 ggagtcacta atgacaactt ttaccgacga cgacaaggga ctgattaaag aaatcagaga
      241 gcgaatcggc agcttggacg tgcgagacaa tattgaacgc cgggcttatg aaatcgcact
      301 ggcatcgctg gaagctgggg ttgttgcatg gaggtatcgt tacgtgaaaa aagacgttac
      361 ggactctcag ggtaaaacgt gggttggtga ctggaaatac gtgccgacaa aagaagactg
      421 caacgacagg ccgaactatg aaattcagga actctacaac ttaccacctg cgatagcagc
      481 accaacaaga ggactggtaa acgcagtacg cttctacgat caggtaaagc acacgaatcc
      541 tcccgtcgaa acaggggcat ggaaagacgc tattgactgg gtgctcaaag aggcttgcca
      601 ggctgtaaac attgacgcca aaggagagtg agatgacaac atttaccgac gacgacaaga
      661 aactaattaa agaaatcagg gagcgaatcg gcagcctgga cgtgcgagac aatattgagc
      721 gccgggctta tgaaattgct ctggcatcac tggaagcaga agcgccaaat ttgcctggtg
      781 gtttcacaat tgaagaagca aaagaattac acgaaaatct ggtaaaaagt cacgtaagca
      841 aggctttaag tggcgaaaaa atgaaaaaag aaaatcgtga tgccgatttg cgctggattc
      901 atggcgtaat agttcaggcc gcgtggtttg tgaaagcatc actagggcag aatgcatagt
      961 gcgtacctgc ttttctcctg gcaacggtgg atcttccgct tcctcgtcac tcgctcgcta
     1021 tgctcggtcg ctagactgcg gcgagcggtt agctatcaaa aaaaatcccc gcacaacgcg
     1081 gggataggtc ataaaaacac atacattgca ctacagtgta gtattcaata agccttacaa
     1141 tgtaaagaca gaaacacctt tacactgtaa atatatgcct gcgatattct tcagcgtcct
     1201 catgacccga tagccggact acggcctgtc taatgttaaa tcgacatcaa ccaaaaacca
     1261 gctatcacag tgcttataat tggagtgatc cgaataagtc aaacaccctt catcatgaat
     1321 atcatcaaca gaataagcac cgcttaatac catatcttcc acgattgcca atatcgcttc
     1381 acgcgccata gcgggtattt ctgcctgaga atcagccgca gaaacacatc caaaaccaaa
     1441 acaatcaaaa gccggaacag tgatgccgta tgcagtctga tcatctttcg gtgtttcgac
     1501 accaacagaa aaaaacatga ttatctccac agaaggcagt gatttacaac gtggtgcact
     1561 gtaaagacac aaagaccttt acaatgtaag cgagaatttt t
//
LOCUS       DAPGOF010000336         1586 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.344, whole genome shotgun sequence.
ACCESSION   DAPGOF010000336 DAPGOF010000000
VERSION     DAPGOF010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.344"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>1586
                     /locus_tag="RHV13_004732"
     rRNA            <1..>1586
                     /locus_tag="RHV13_004732"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          400 a          348 c          505 g          333 t
ORIGIN      
        1 gtaggggagc gttctgtaag cctgcgaagg tgtgctgtga ggcatgctgg aggtatcaga
       61 agtgcgaatg ctgacataag taacgataaa gcgggtgaaa agcccgctcg ccggaagacc
      121 aagggttcct gtccaacgtt aatcggggca gggtgagtcg acccctaagg cgaggccgaa
      181 aggcgtagtc gatgggaaac aggttaatat tcctgtactt ggtgttactg cgaagggggg
      241 acggagaagg ctatgttggc cgggcgacgg ttgtcccggt ttaagcgtat aggctggttt
      301 tccaggcaaa tccggaaaat caaggctgag gcgtgatgac gaggcactac ggtgctgaag
      361 caacaaatgc cctgcttcca ggaaaagcct ctaagcatca ggtaacatca aatcgtaccc
      421 caaaccgaca caggtggtca ggtagagaat accaaggcgc ttgagagaac tcgggtgaag
      481 gaactaggca aaatggtgcc gtaacttcgg gagaaggcac gctgatatgt aggtgaagcg
      541 acttgctcgt ggagctgaaa tcagtcgaag ataccagctg gctgcaactg tttattaaaa
      601 acacagcact gtgcaaacac gaaagtggac gtatacggtg tgacgcctgc ccggtgccgg
      661 aaggttaatt gatggggtca gcgcaagcga agctcttgat cgaagccccg gtaaacggcg
      721 gccgtaacta taacggtcct aaggtagcga aattccttgt cgggtaagtt ccgacctgca
      781 cgaatggcgt aatgatggcc aggctgtctc cacccgagac tcagtgaaat tgaactcgct
      841 gtgaagatgc agtgtacccg cggcaagacg gaaagacccc gtgaaccttt actatagctt
      901 gacactgaac attgagcctt gatgtgtagg ataggtggga ggcttagaag tgtggacgcc
      961 agtctgcatg gagccgacct tgaaatacca ccctttaatg tttgatgttc taacgttgac
     1021 ccgtgatccg ggttgcggac agtgtctggt gggtagtttg actggggcgg tctcctccta
     1081 aagagtaacg gaggagcacg aaggttggct aatcctggtc ggacatcagg aggttagtgc
     1141 aatggcataa gccagcttga ctgcgagcgt gacggcgcga gcaggtgcga aagcaggtca
     1201 tagtgatccg gtggttctga atggaagggc catcgctcaa cggataaaag gtactccggg
     1261 gataacaggc tgataccgcc caagagttca tatcgacggc ggtgtttggc acctcgatgt
     1321 cggctcatca catcctgggg ctgaagtagg tcccaagggt atggctgttc gccatttaaa
     1381 gtggtacgcg agctgggttt agaacgtcgt gagacagttc ggtccctatc tgccgtgggc
     1441 gctggagaac tgaggggggc tgctcctagt acgagaggac cggagtggac gcatcactgg
     1501 tgttcgggtt gtcatgccaa tggcactgcc cggtagctaa atgcggaaga gataagtgct
     1561 gaaagcatct aagcacgaaa cttgcc
//
LOCUS       DAPGOF010000337         1545 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.345, whole genome shotgun sequence.
ACCESSION   DAPGOF010000337 DAPGOF010000000
VERSION     DAPGOF010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.345"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            13..1077
                     /locus_tag="RHV13_004733"
     CDS             13..1077
                     /locus_tag="RHV13_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HDV1244157.1"
                     /translation="MLAANVAHAAEVYNKDGNKLNLYGKVTALRYFTDDKRDDGDKTY
                     ARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAGLKISEFGSLDYG
                     RNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGVATYRNYDFFGLIEGLNFAAQ
                     YQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKSDRTNAQERAAAN
                     PLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNKAQSFEVVAQYQF
                     DFGLRPSLAYLKSKGRDLGRYGDQDMIEYIDVGATYFFNKNMSTYVDYKINLIDESDF
                     TRAVDIRTDNIVATGITYQF"
     gene            1241..>1545
                     /locus_tag="RHV13_004734"
     CDS             1241..>1545
                     /locus_tag="RHV13_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HDV1244158.1"
                     /translation="MIMTNINTACIKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     LNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDFA
                     "
BASE COUNT          449 a          321 c          328 g          447 t
ORIGIN      
        1 gttgtaactg gtttgttagc tgcgaacgta gcacacgctg ccgaagtcta taacaaggat
       61 ggtaataaac tcaaccttta tggcaaggtt accgctctac gttattttac tgatgataag
      121 cgtgacgatg gtgataaaac ttatgcccgt ctcggcttta aaggagaaac gcaaatcaat
      181 gatcaaatga ttggttttgg tcactgggaa tatgatttta aaggctataa cgatgaagcc
      241 aacggctcgc gcggcaacaa gacccgtctg gcctatgctg gtttaaaaat tagtgaattt
      301 ggctctctgg actatggtcg taactacggt gtcggctatg acattggttc atggaccgat
      361 atgttgccag aatttggtgg cgatacctgg agtcagaaag atgtcttcat gacataccgt
      421 accaccggtg tagcaaccta tcgcaactac gatttctttg gcttaattga agggctgaac
      481 tttgccgcgc aatatcaagg caaaaatgaa cgtactgaca acagtcatct ttatggtgct
      541 gactacacgc gtgccaacgg tgacggtttc ggtatctcct caacttatgt ttatgatggc
      601 tttggtatcg gtgcggtata taccaaatcc gatcggacaa atgcgcagga aagagccgct
      661 gctaatcctc tcaatgcctc cggtaagaat gcagaactgt gggctacagg tataaaatat
      721 gatgccaaca acatctactt tgcagctaat tacgctgaaa cattaaacat gaccacctat
      781 ggcgatggtt atatttctaa caaagcacaa agttttgaag tggtggcgca atatcaattc
      841 gacttcggct tgcgcccctc actcgcttac ctgaaatcga aaggcagaga tctgggccgc
      901 tacggcgacc aggacatgat tgagtatatc gacgttggtg cgacgtattt cttcaacaaa
      961 aatatgtcga cctatgttga ttataaaatc aacctgattg atgaaagcga ctttacccgt
     1021 gccgtagata ttcgcaccga taacatcgtc gcaacgggca ttacctatca gttctaacat
     1081 tctctcctga ccagcccttc tctgggctgg tttttgattt aagtgctgct gtatgactcc
     1141 ttaagtaaaa atgagcaatt tttgatattt ctggtcattc acctcaatta atattattcc
     1201 ataccctccg gggacatctt attataatat ttattgatga gtgattatga ccaatattaa
     1261 tacagcttgt ataaaaaata atgccagtta tcaacttaat aacgcattac ctaacaaaga
     1321 aaccatctcc agcaattttt gtgaacgact agaacaatgg ggtaataagt cgcttaataa
     1381 tggtgaagaa agagcaattg ccgtggagcg aattaaagaa gcttacaatt cgaacatggc
     1441 atctcttgat ttgtcatatc ttgatttgag tgagttgccg cctatccctt cgacagttaa
     1501 tacgttaaat ctggaaaaca attgtctaac ttgtcttgac tttgc
//
LOCUS       DAPGOF010000338         1445 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.346, whole genome shotgun sequence.
ACCESSION   DAPGOF010000338 DAPGOF010000000
VERSION     DAPGOF010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.346"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            465..692
                     /locus_tag="RHV13_004735"
     CDS             465..692
                     /locus_tag="RHV13_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1244159.1"
                     /translation="MQAAPQREETEWRVQSKRELMPAYRGEAGQQVNIKIMEYSERNV
                     RQLASNEQEEYIPRKINVGVINTPTLIRSDY"
     gene            complement(727..867)
                     /gene="ykgO"
                     /locus_tag="RHV13_004736"
     CDS             complement(727..867)
                     /gene="ykgO"
                     /locus_tag="RHV13_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HDV1244160.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(867..1130)
                     /locus_tag="RHV13_004737"
     CDS             complement(867..1130)
                     /locus_tag="RHV13_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDV1244161.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGS"
BASE COUNT          421 a          287 c          327 g          410 t
ORIGIN      
        1 ctttgccctg aagtgttagg aatagtctta atgcgctgtg tgatggacta gtaaaaaaac
       61 aagttttaag ggtaagggat agaatataaa actataatga gctgtcattg aagatgattt
      121 gccaatcagt aagaaatttt aatggattaa catgccgcac aaaatggtta taggtgttca
      181 ttacaataag tgatggaaaa ttgaacgggc gtatgtacgt catctgttcg gtaagccccc
      241 aagagccaga ttaagtgaag ttaaacgaag gctgccagct gtcacaggaa cgatggcctc
      301 tttgtcaggg cactgagtgc agttatctgt gcgcgttact ctaactggca ctcgaacgtc
      361 ggcgacgggg ttaccggtgg atcagactgt tcctgtggcc taaacgtttt tatgttagta
      421 ccaacagatt taccacattt atcatcttaa cagtttgagt gaaaatgcag gctgcgccgc
      481 aaagggagga gactgagtgg cgtgttcagt ctaaaagaga gttgatgcca gcatacaggg
      541 gcgaagctgg tcaacaagtc aacattaaga taatggaata ttcggagaga aacgtcaggc
      601 agttggcctc aaatgaacag gaagaatata tccccaggaa aattaatgtt ggtgttataa
      661 acacaccaac attaatccga tctgattatt aatcagacaa atatcgttaa tccgtcgaat
      721 tttgaatcaa cgttttttct tacgaccctg aacggcctta aaacgtggat tagatttaca
      781 aatcacatat agccgtcctt ttcgcttcac aatctgacag tctggatggc gttcttttgc
      841 ggtacgcaga gagttaagaa ctttcatcac gacccctttt tcgtgctaac aaaacgacca
      901 aaacgttggg tgaatcgtgc aacatttcct tctgatgcca ctgttctcag cttccctgta
      961 tagaacgggt gcgatttaga agagacatca attgtcacgt acggatacgt tacgccatcc
     1021 agctcaatct cacggtctgt tttgatagtc gagccgattt taaagtactc atcaacactg
     1081 gtgtcgtgga acaccacagt acgatactca ggatggatat tgggcttcat catttttacc
     1141 tgttatgtta taacataacc ataaagatac atgctcatcc ggatcatttc aacacccaat
     1201 ccctgtgact ctttgtggat gtggaatttt cgcaacgggg aactctggca ttgttagcag
     1261 ccattggtga cgctcccata aacgcctgga tgagtgattg atgatggttt tgctgagagt
     1321 atggattttt cgagaatgga ggttatctcc actttactgg ccgatgattt atcgcagtat
     1381 gattgattac ctgacagcgt atattcctct cccacggata taataattac ttacaaaaag
     1441 ggaga
//
LOCUS       DAPGOF010000339         1428 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.347, whole genome shotgun sequence.
ACCESSION   DAPGOF010000339 DAPGOF010000000
VERSION     DAPGOF010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.347"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..403
                     /locus_tag="RHV13_004738"
     CDS             <1..403
                     /locus_tag="RHV13_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDV1244162.1"
                     /translation="LMACIDLLSFLLPRLYTVTFIFSGLLYQTWNNNILSGLFCAMLM
                     FFIMLFVRLYFAYKNGTESFGMGDVLLIAGTGVWFPTPEIACSIVFIAVIGGIIFFTL
                     GGLNKQKKYIPFGPFLCGGMFVYSLVPGILF"
     gene            416..>1428
                     /gene="pilV"
                     /locus_tag="RHV13_004739"
     CDS             416..>1428
                     /gene="pilV"
                     /locus_tag="RHV13_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HDV1244163.1"
                     /translation="MKKTDKGVSLLEVLLVIGIMVMVIPKVYENIENHLNNVRWQNAA
                     EHANTYNTAVRNYVADNASTLLAGSLPKTITPATLIQKGYLKSGFSESNFGQSYITGI
                     AKNSKTSRLEALTCSNGGQSLSEAGMRSVASMIEGLGGYINSSKQAIGAGGGWSDTPS
                     NYGLNCATGHIAMALVGADLQESDRLYRYSITNRPDLNRMHTAIDMNSNNLNNVGTLN
                     GNAAALSGDISARNGTFSGAISGNTATTNGDITSNNGWLVTKNSKGWMNSTYGGGWYM
                     SDSSWLRSVNNKGIYTGGQVKGGTVRADGRLYTGEYLQLEKTATAGASCSPNGLVGRD
                     STGA"
BASE COUNT          389 a          321 c          336 g          382 t
ORIGIN      
        1 attaatggcc tgtatagacc ttctttcatt tctactacca cgcctttata ccgtgacatt
       61 tatcttctcc ggcctgttat atcagacatg gaataacaat atattgtctg gtttgttttg
      121 tgcaatgctg atgttcttca ttatgctgtt tgtaagactt tactttgcat ataaaaacgg
      181 aacggaaagt ttcggcatgg gagatgtgct acttattgca ggaactggcg tatggtttcc
      241 aacgcctgag attgcctgtt caattgtatt tattgccgtt atcggcggaa ttattttctt
      301 cactcttggc ggattaaaca agcaaaaaaa atatattcca tttggtccgt tcctttgtgg
      361 cggaatgttt gtttactcac tcgtaccggg aatattattc tgaggattaa gagcaatgaa
      421 aaagacagat aaaggtgtat ctctccttga ggtattactt gtcatcggca taatggtgat
      481 ggttatccct aaagtttatg aaaacattga aaaccatctg aataatgttc gctggcagaa
      541 tgcagcagaa cacgcaaata cctataacac cgctgttcgt aattatgttg ctgacaatgc
      601 atcaacacta cttgcaggct ctttacccaa aacaatcacg ccagcaacac tcattcagaa
      661 aggctatctg aaatctggtt tttctgaatc caattttgga caaagctaca ttaccggtat
      721 tgcaaagaac agcaaaacat cccgtctgga agccctgacc tgtagtaatg gcggtcagtc
      781 cctgtcagaa gccggaatgc gctcggtagc aagcatgatt gaagggcttg gcggctacat
      841 caacagcagc aagcaggcga ttggcgcggg tggcggctgg tctgacacgc cttcaaatta
      901 cgggctgaac tgcgcgaccg ggcatattgc aatggcactt gttggcgctg atttgcagga
      961 aagcgaccgt ttgtatcgtt actccataac taatcgtccc gaccttaaca ggatgcatac
     1021 ggcgattgat atgaacagca acaatctgaa caacgtcggc actttaaacg gcaacgccgc
     1081 cgcgctctcc ggcgatatca gcgcacgtaa cggtacgttc tctggcgcaa tttcaggaaa
     1141 tacagccaca accaacggtg atatcacttc gaataatggc tggctggtca caaaaaacag
     1201 caaaggctgg atgaacagca cctatggtgg cggctggtat atgtccgatt cgtcctggtt
     1261 gcgcagcgtg aataataaag ggatttacac tggtggccag gtaaaaggtg gaactgtacg
     1321 cgctgatgga cgcctctata caggcgagta tttacagctt gaaaaaacag caacggctgg
     1381 cgcttcctgc tcccctaacg ggcttgtagg ccgcgacagt acaggggc
//
LOCUS       DAPGOF010000340         1423 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.348, whole genome shotgun sequence.
ACCESSION   DAPGOF010000340 DAPGOF010000000
VERSION     DAPGOF010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.348"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..532
                     /locus_tag="RHV13_004740"
     CDS             <1..532
                     /locus_tag="RHV13_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HDV1244164.1"
                     /translation="ILRSLLDANPNIYLVGLVLIVAIVVKSFVGQSGKGARDNYSLNN
                     YTLVPSVMTKAEMTFYLSLISTVGDKYTVMVKVRLADIITVKKSSTNYMAHFGKIKAK
                     HVDYLLCDKTDLKPLLAIELDDKSHQREDRIARDKLVDGIFKAVGLPVIHHPVKNTYS
                     QSNLIMIISEAIYNRH"
     gene            594..956
                     /locus_tag="RHV13_004741"
     CDS             594..956
                     /locus_tag="RHV13_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001426321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1244165.1"
                     /translation="MEYGMNVKKLFALKAPCKNCPFLKENGIELVEGRLDSIKEDLIN
                     NDETPFFCHKTTYSSGGFYDEETEAYVNSGQESYCMGAMAYLYAKNRLNVPTRIGLVM
                     GMCDIEDIKNTIPFIKIE"
BASE COUNT          449 a          278 c          296 g          400 t
ORIGIN      
        1 tatcttacgt agcctgctcg atgcgaaccc aaacatttat ctagtgggtt tagttctgat
       61 agtagcaatc gtcgttaagt catttgtagg gcagtcagga aaaggggcaa gggataatta
      121 ttcgctcaat aactatactc tcgttccgtc tgtcatgact aaagccgaaa tgacatttta
      181 tctttcatta atatcgactg taggcgataa gtacacagtc atggtaaagg ttagacttgc
      241 cgatatcatc actgtaaaaa agagcagcac taattacatg gctcactttg ggaagattaa
      301 agctaaacac gttgactatc ttctctgtga taaaactgat ttaaaaccct tgttggctat
      361 tgagcttgat gataaaagcc accagcgtga agaccgtatt gctcgtgata agttggttga
      421 tgggatattt aaagctgttg gtttacctgt gattcatcac ccggttaaaa acacgtacag
      481 tcagagtaat ttgattatga ttatttctga ggcaatttat aaccgtcact gattaaagtt
      541 aataacttta cctgccgtgg ttactgtgaa cgataccgca gtaaccattc tcaatggaat
      601 atggtatgaa tgtaaaaaaa ttattcgccc taaaagcccc atgtaaaaac tgcccattcc
      661 tgaaagaaaa tggcatcgag ttagtcgaag ggcggcttga tagcatcaaa gaggacttga
      721 tcaataatga tgaaacccca tttttttgtc acaaaacaac gtattcatca ggtggttttt
      781 atgacgaaga aacagaggca tatgtaaact ccgggcagga gtcttactgt atgggtgcaa
      841 tggcatacct ctatgcaaag aaccgcctga atgttccaac ccgaatcggc cttgttatgg
      901 gaatgtgtga cattgaagac atcaaaaaca ccatcccttt catcaaaata gagtaaaaat
      961 cagcgtcaaa aaatgcggct gtccgggggg ctatgaggct atctaaattt gagcactgat
     1021 taatcataga cactgccata gatctggtta tgactggcag ggacttgttt gtggaaaaaa
     1081 acgacttcac acccaacaaa ttaatgccaa gcaaagcaca aatttcaaga agaacagcaa
     1141 ccttcttgag gagccagttt acctccgttg gacgtcaaga aagggtttct taataggtgt
     1201 ggtgttctct gggccagcta gcgatatgcc gatcatagac aacttttcca ccaactctga
     1261 atcaatggga tgaatgtaaa tcatgatttc tgaacacttc atgatggtga ctggaccaaa
     1321 atgcaagctg gattctccgc aaaaatcgcg caccggataa ctactttgct gttacacact
     1381 tagtgcactt aatcattgtt atgttaaatt cgaactgtat ata
//
LOCUS       DAPGOF010000341         1405 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.349, whole genome shotgun sequence.
ACCESSION   DAPGOF010000341 DAPGOF010000000
VERSION     DAPGOF010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.349"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>1405
                     /gene="espL4"
                     /locus_tag="RHV13_004742"
     CDS             <1..>1405
                     /gene="espL4"
                     /locus_tag="RHV13_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
                     /protein_id="HDV1244166.1"
                     /translation="QYINDIYQNVNYKPHQDDYSSAEKFLTHFNKKCKNQTLALISSR
                     PEGRCVAACGDFGLVMKAYFDKMESNDLSVMAAILLVDNHALTVRLRIKNTTEGCIHY
                     VVSVYDPNVTNDKIRIMSESKEDIKHYSLMDFMNVDYSLLKWSNDHVINQSVAIIPAL
                     PKEQLLMLKGSVDEITPPLSPATMNLLMAIGQNHQLKQLMIQLQKMPELHRTEMLTAY
                     NSINLPGLYLAINYGNADIVETIFNSLSEPGYEGLLSKKNLMHILEAKDKNGFSGLFL
                     AISRKDKNVVTSILNALPKLAATHHLDNEQVYKFLSAKNSTSSHVLYHVMANGDADML
                     KIVLNALPLLIRTCHLTKEQVLDLLKAKDFYGCPGLYLAMQNGHSDIVKVILDALPSL
                     AQEINISASDIVDLLTAKSLARDTGLFMAMQRGHMNVINTIFNALPTLFNTFKFDKKN
                     MKPLLLANNSNEYPGLFS"
BASE COUNT          452 a          265 c          270 g          418 t
ORIGIN      
        1 caatatatta atgatattta tcagaatgtt aattacaaac cccatcaaga tgattactct
       61 tcagctgaaa aatttctcac gcatttcaac aaaaaatgca aaaaccagac tttggcgttg
      121 atttccagcc gtcctgaggg gcgttgcgtt gctgcctgcg gtgatttcgg gctagttatg
      181 aaggcatatt ttgacaagat ggaatcaaat gacctcagtg ttatggctgc catattattg
      241 gtagataacc atgctttgac ggtccggcta agaataaaga acacaactga aggatgtatc
      301 cattacgtgg tttcggttta tgatcctaat gtaactaacg ataaaataag aatcatgagc
      361 gaaagtaaag aggatattaa acactattca ctgatggatt ttatgaatgt agattatagc
      421 ctcctgaaat ggtcaaatga tcatgttatt aaccaatctg ttgcaataat tccagcactt
      481 ccaaaagaac agctattgat gctaaaagga tctgtggatg aaataacccc tccattatca
      541 cctgcaacga tgaatttgct aatggcaatt ggtcagaatc accaacttaa gcaactgatg
      601 attcaactcc agaaaatgcc agaactacat agaacagaaa tgttgactgc ctataatagt
      661 attaacctgc caggtttata tttggctata aattatggta atgcggatat cgttgagact
      721 atttttaatt cattgtcaga gccaggatat gaagggttac tctcgaaaaa aaatctcatg
      781 catattctgg aggcaaaaga taaaaatggt ttttctggat tatttttagc gatatcacgt
      841 aaggataaaa atgttgtaac ctcgattctg aacgccttac ctaaactggc cgcaacacat
      901 catttagata acgaacaagt gtataaattc ctgagtgcca aaaatagtac gtccagccat
      961 gttttatacc atgttatggc gaatggtgat gccgacatgc tgaaaattgt tttgaacgcg
     1021 ttacctttgt taattcgcac atgtcatttg actaaagaac aggtactcga tctcctgaag
     1081 gcaaaggatt tttatggttg cccaggacta tacctggcga tgcaaaatgg acatagcgat
     1141 atcgtgaaag ttattcttga cgcattgccc agcctggccc aggaaattaa catttcagct
     1201 tccgatattg tagatcttct gaccgctaaa agtcttgcgc gcgacacggg tttgtttatg
     1261 gccatgcagc gcggacatat gaacgttatt aatactattt ttaacgcatt acccactctg
     1321 tttaatacgt ttaaattcga taaaaaaaat atgaagcccc tcctcctggc aaataattct
     1381 aatgaatacc caggtttgtt ttcag
//
LOCUS       DAPGOF010000342         1399 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.350, whole genome shotgun sequence.
ACCESSION   DAPGOF010000342 DAPGOF010000000
VERSION     DAPGOF010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.350"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..338)
                     /locus_tag="RHV13_004743"
     CDS             complement(<1..338)
                     /locus_tag="RHV13_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HDV1244167.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFQEYCSTLAGAGVFR
                     WVTDVNHNKR"
     gene            complement(559..1290)
                     /gene="bluR"
                     /locus_tag="RHV13_004744"
     CDS             complement(559..1290)
                     /gene="bluR"
                     /locus_tag="RHV13_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HDV1244168.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
BASE COUNT          369 a          316 c          307 g          407 t
ORIGIN      
        1 ctgcgtttgt tatgattaac atcagtaacc caacggaaaa cgcccgcgcc tgccagtgtt
       61 gaacagtatt cctgaaatgt agattttccg caaatatgaa gcaatgcggc ctcttttatt
      121 ttagcagggt tcttagtcgt actaactttt aacaggtttc tggttcctct taataacaaa
      181 accgtgtcat cgtgagtaat aattctgatg ttatccgtag ccagataata aatgtaatgt
      241 gcaatacggt gatgttttaa ttctgaataa aaccaggaga agttttgctc ttttctcact
      301 tgctcaaaca tcttttgaaa aacaacgacc tgatccatca ggataataac ctcttgttag
      361 ttgtgagact gcgtagtgtg cacgatcggt tttaccactt caatctggtc tgtcctttgg
      421 ctgtgatatg tacagagtgt gatagaggga ttacctgaat tctcccggtg agcattttgc
      481 aacggatcag ctccggtaca aatgctgttg tgggttcaga ttataacatt ctgtctaagg
      541 ggcgggataa aggtgaaatt agggggcatg aaagatgact ttataacctt gctcacccca
      601 gtgttgtaaa agttcgtttt gccttctcgt tggtgccatg cctgtccaga caatcaatgt
      661 ttgcgtcggg aacagttccg ggcgcggcga ttcaatggga tcagcaagaa cagaaatgtg
      721 ccatcctgac aatgataaac gccatgcttc cagccacaat cgtgctctct cttcaacatt
      781 ccacgccatc agaatggctt ctttaccggg cttacggcgc atttcgaaaa gcgaggttgc
      841 tgcgtattca attaatgcgc cgtcaaacat actgctcata atgcgggagg tgttgtgatc
      901 aagcacgaga cgctggcgaa caggaaggta aacatgatta atcaattgat ctactgggta
      961 ctctcgaccc agtgaaataa ttctcgcgcg tagtttggca ggattagcca tacgaagaat
     1021 tgacatcatc tcttcttgca ggcggctcca gtcatcttcc gtatcctggc tggtggtttc
     1081 cagtaatgct ttaactttgc ctacagggac gccattactt atccaacgct tgatctcttc
     1141 gatgcgttgt atgtcttctt catcaaagag tcggtgtccg ccttcactgc gctgtggttt
     1201 taacaaaccg tagcggcgtt gccaggcccg gagagtgaca ggattaatcc cgcaacgttc
     1261 agcaacatca ccaatgctgt aataagccac aattcctcct tgcggtcaca aatctccgtc
     1321 gcctgtacac gacccaataa tactttgtac aatatacgct aaaattgtac aaagtataaa
     1381 taagatttca gctaaattg
//
LOCUS       DAPGOF010000343         1397 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.351, whole genome shotgun sequence.
ACCESSION   DAPGOF010000343 DAPGOF010000000
VERSION     DAPGOF010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.351"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            300..1085
                     /locus_tag="RHV13_004745"
                     /pseudo
     CDS             300..1085
                     /locus_tag="RHV13_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386522.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            1082..>1397
                     /locus_tag="RHV13_004746"
     CDS             1082..>1397
                     /locus_tag="RHV13_004746"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HDV1244169.1"
                     /translation="MSDPLGLQGGEQTYRYVPNPCGWVDPLGLAASSKISSLMDYIGD
                     GRRVSGHTGFLDGVRLSRSQINNITKEMEKLGIKVIRKADKYLPPNARAAFDYGLRNI
                     YLR"
BASE COUNT          358 a          327 c          444 g          268 t
ORIGIN      
        1 acagtggatt tattttaacg gggagattgt tttggcaagg ggaaggtttt ggccatgaat
       61 tattccccgt gacaaacaaa tataatgctt ggagatggaa aaataaaaaa ataatagata
      121 tttcttggta aggaaaatat tccccgaaag aggccacatg gggaatactg ctcccctcac
      181 tggtacggga gtaccgttac gacagcgcgg gtaacgtatc aggcgtcacc agccgggaag
      241 attacggacg ggaaacacag cgggagtacc ggcttgaccg gaacggccag gtcacggcgg
      301 tgacagcctc aggcaccggg ctgggctatg gcgaaggcga tgagtcctat ggctatgaca
      361 gttgtggcta cctgaaggcg cagtctgcgg gcgggcaccg gataagtgaa gagactgacc
      421 agtatgccgg aggccaccgg ctgaaacagg ccggaaacac gcagtatgac tatgacgccg
      481 caggccggat ggtcagccgc accagacacc gtgacggcta tcgcccggaa acagagcggt
      541 tccggtggga cagccgggac cagctgaccg ggtattgcag cgcacagggg gagcagtggg
      601 aataccgcca cgacgccagc ggcagacgaa cagagaaacg ctgcgaccgg aagaaaatcc
      661 gcttcacgta cctgtgggac ggcgacagta ttgcggaaat ccgggaatac cgcgatgata
      721 aactgtacag cgtacggcac ctggtgttta acggctttga gctgataagc cagcagtgca
      781 gccgggtacg gcagccgcac ccgtccgtgg ccccgcaacg aatcatgcgg tgaacgacct
      841 gacgggccgt ccgctgatgc tctttaacag tgaaggtaaa accgtctggc ggccgggaca
      901 gaccagcctg tgggggctgg cactcagcct gcccgcagac acagactacc cggacccgcg
      961 cggggagcgg gacgcggaag ccgaccccgg cctgctgtat gcaggacagt ggcaggatgc
     1021 agaatcgggg ttgtgctata accggttccg atattacgag ccggaaaccg ggatgtacct
     1081 ggtgagtgat ccactggggt tgcagggagg ggagcagact taccggtatg tgccgaatcc
     1141 ttgtgggtgg gttgatccgc tgggattggc tgcaagttct aaaatcagca gtttgatgga
     1201 ctatattggc gatggtcgtc gtgttagtgg gcatacgggt ttcctggatg gggttcgttt
     1261 atcacgtagt caaataaaca atattactaa agaaatggag aagctaggaa ttaaagtaat
     1321 aaggaaagca gataaatatt tgccaccaaa tgctagggca gcttttgatt atggtcttcg
     1381 caatatttat cttagga
//
LOCUS       DAPGOF010000344         1356 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.352, whole genome shotgun sequence.
ACCESSION   DAPGOF010000344 DAPGOF010000000
VERSION     DAPGOF010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.352"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..759)
                     /locus_tag="RHV13_004747"
     CDS             complement(<1..759)
                     /locus_tag="RHV13_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDV1244170.1"
                     /translation="MDLLAESITEVAVSGEIANTHRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVRIYKVSNAFINIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYK"
     gene            complement(785..>1356)
                     /gene="rfaY"
                     /locus_tag="RHV13_004748"
     CDS             complement(785..>1356)
                     /gene="rfaY"
                     /locus_tag="RHV13_004748"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HDV1244171.1"
                     /translation="IDDTKVSLIDTKYGRYVFKVFAPKTKRNERFLKSFVKGDYYQNL
                     IVETDRVRSAGLTFPNDFYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPENVKAE
                     IKASMEKLHALNMLSGDPHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGI
                     ANEIKDYGYYSVIYRTKLRKFIKKLKGKA"
BASE COUNT          428 a          281 c          231 g          416 t
ORIGIN      
        1 tttataatct tcatcgcttt tattttttaa ctcataatcg atgccataaa agcaatcata
       61 atcgccgcta ataaaaatac aatgaccaaa gaacagaata tttaaaatat cctgatcaag
      121 gcatttatac ttcgtgtgct gcgggtcact tgccagcata gcaatggctt tattcataaa
      181 atcatttttc gcccatgctt caagctgcag atatactaca ccggcgttga aataattccc
      241 tggaagtccc tctatagcca gacgcttcgc tggtttttcc tgcataaaag gcacatcttt
      301 aataacagct gcaaacttat ccccatcaaa attaatatca agcaatccag ctaatgagcc
      361 tttacagatg acatcagcat cgatatataa cagattttca atagtttcac tcagatactg
      421 gaacgcaact aaacggaaga atgttgcata agaccactgt ttcaggctgg ggaagatatt
      481 aataaaggca ttggagactt tataaattct tacttcaacg ttagcatttt tgctggtaaa
      541 agcattaaca cgttgtagat aatcttcatc aatgtagtcg gtaaagagat gaaacttaac
      601 cgcaaggtcc gggttatgca taacaactga ttgcatagat actgccgcac caaataaaaa
      661 gttgcggtca ataccgtaag cgatatttaa cacacgatgg gtgttagcaa tttccccaga
      721 gacagcgact tcagtaatac tctcagctaa taaatccatg ttggttccgt tttgactgtg
      781 tggtttacgc tttgcctttt aattttttaa taaatttgcg tagtttggtt ctgtagatta
      841 ctgaatagta gccataatct tttatttcgt tggcaatacc caggtgacgt tccatggcca
      901 gacgatcacg agctttacgc tctgccgtac agcttttacc ggataggtca attatcctga
      961 caccatcttt actaacgata aagttacccc ggtgtgggtc gcccgatagc atatttaagg
     1021 catgcaattt ttccattgaa gccttaattt ctgctttaac attttctgga ataatcggca
     1081 tatcattgag ctctacgccc tcgacatact cgataagcat gataaaaaca ctggcatagt
     1141 tgaaaatttt acgctcagcc agaaaataaa aatcattagg aaaagtaaga cctgcacttc
     1201 tgacacgatc ggtttcaacg atcaaattct gatagtagtc accttttaca aaagatttta
     1261 aaaaacgttc attccttttc gttttcggag caaagacttt aaaaacatag cgaccatatt
     1321 tcgtatcaat caacgatact ttggtatcat caatat
//
LOCUS       DAPGOF010000345         1352 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.353, whole genome shotgun sequence.
ACCESSION   DAPGOF010000345 DAPGOF010000000
VERSION     DAPGOF010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.353"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..726
                     /locus_tag="RHV13_004749"
     CDS             <1..726
                     /locus_tag="RHV13_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311760.1"
                     /note="may be involved in the export or assemby of
                     proteins involved in the entry of Salmonella into host
                     cells; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscC/YscC/HrcC family type III secretion system
                     outer membrane ring protein"
                     /protein_id="HDV1244172.1"
                     /translation="DWSGTIKIGSSISASFNNSGSISTLDGTQFIATIQALAQKRRAA
                     VVARPVVLTQENIPAIFDNNRTFYTKLVGERTAELDEVTYGTMISVLPRFAARNQIEL
                     LLNIEDGNEINSDKTNVDDLPQVGRTLISTIARVPQGKSLLIGGYTRDTNTYESRKIP
                     ILGSIPFIGKLFGYEGTNANNIVRVFLIEPREIDERMMNNANEAAVDARAITQQMAKN
                     KEINDELLQKWIKTYLNREVVGG"
     gene            731..>1352
                     /locus_tag="RHV13_004750"
     CDS             731..>1352
                     /locus_tag="RHV13_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopN family type III secretion system gatekeeper
                     subunit"
                     /protein_id="HDV1244173.1"
                     /translation="MAIHVEHVGVLERAREVSRLEDIITEDNEDIEAEMPKMGDDPAG
                     KEARFLQATDEMSAALTQFMKKKIYEEQLANFLDGEEYVLEDQPIEKTDKVMEALKAA
                     TTHDYEVYSFAKKLFPDESDLVVVLRAILRKKQISENVRLNAEALLRKVNQETTKKFI
                     NSGINSALKAKLFGQALSLNPKLLRASYRQFLMAEDDAVDTYVEWIG"
BASE COUNT          464 a          230 c          309 g          349 t
ORIGIN      
        1 gactggtcgg gcacgataaa aattggatcc agcataagtg catcatttaa taattcaggt
       61 tcaattagta cccttgatgg gacacagttt attgcaacaa tacaggcact tgcgcaaaaa
      121 agaagagccg ccgttgttgc tcgtccagtc gttttaacac aagagaatat tcctgctata
      181 tttgataata accgcacttt ctatactaaa ttagtcggcg agcgtactgc ggaattggat
      241 gaagtaacct atggtacgat gattagcgtg ctaccacgat ttgctgcgcg aaatcaaatt
      301 gaattactct tgaatattga agatggaaat gaaatcaatt ccgacaagac caatgttgat
      361 gatctgcctc aggttggtag aacacttata agtactattg cacgcgtgcc gcaagggaaa
      421 agtcttttga ttgggggata tacacgcgat acgaatacct atgaaagtcg caagatccca
      481 atattaggca gcattccatt tattggtaaa ttatttggtt atgaaggaac aaatgcgaat
      541 aacatcgttc gtgtctttct tattgagcca agagagatcg atgagcgcat gatgaataat
      601 gcgaatgagg ctgcggttga tgccagagcg attacacagc aaatggcaaa aaataaagaa
      661 attaacgatg aattactgca gaaatggata aaaacttacc tgaatcgtga agtcgtcgga
      721 ggatagtgaa atggcgattc atgtagagca tgtcggtgta ttagaaagag ccagggaagt
      781 ttctcgctta gaggacatta tcacagaaga taatgaagat attgaagctg aaatgcctaa
      841 aatgggggat gatcccgcag gtaaagaagc tcggttctta caagctactg acgagatgtc
      901 agcagcgtta acgcagttta tgaagaaaaa aatctacgaa gaacaactgg ccaattttct
      961 ggacggagaa gaatatgtcc tggaagacca gccaatagaa aaaaccgaca aagttatgga
     1021 ggcgcttaag gctgcaacta cccatgacta tgaagtgtat agctttgcta aaaaattatt
     1081 cccagatgaa agcgatctgg tagttgtgtt acgtgcaatt ttacgtaaaa aacaaatatc
     1141 agaaaatgtc cggctaaacg ctgaagcatt acttagaaaa gttaatcaag agactactaa
     1201 aaaattcatt aatagcggca ttaactcggc attaaaagca aagttatttg gccaggcgct
     1261 gtcattaaat ccaaaattac taagggcgag ttatcggcaa tttttaatgg cagaagacga
     1321 tgctgtagat acttatgtcg aatggattgg ta
//
LOCUS       DAPGOF010000346         1329 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.354, whole genome shotgun sequence.
ACCESSION   DAPGOF010000346 DAPGOF010000000
VERSION     DAPGOF010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.354"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..826)
                     /locus_tag="RHV13_004751"
     CDS             complement(<1..826)
                     /locus_tag="RHV13_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1244174.1"
                     /translation="MKLATDLVKAILIRNGVEPVNISESSKLSLKQPLTELKFSLYLR
                     TEHLSHFLPGYVYSIKECPLSYDIGIEIQKIFESLHLLNEEFESLGFVSVWIEMHQSI
                     FEQSRFKKSNFTFREEEVELAKGLVCSHNSQISNAAYFWLQHFEEFMIYVRNKLIDLF
                     REAYDLILGMQQVFFIREKEELLKTISYGDELYEVKFMAAELYSIEQNLKCSESISTK
                     SQASAYINRIREFVRSGWRQGYIVCHHKRSNQISSLSLPMYLKDTSGRSARKRVKRL"
BASE COUNT          410 a          287 c          236 g          396 t
ORIGIN      
        1 taagacgttt tactcgcttt cgagcacttc ggcctgacgt atctttcaag tacatcggta
       61 atgacaatga tgaaatttga ttacttcgct tatgatggca gactatatag ccctgcctcc
      121 accccgaacg cacaaactca cggatgcggt taatatatgc cgaagcttga gattttgtac
      181 ttatcgattc agaacacttt aagttctgtt cgatagagta aagctcagcc gccatgaatt
      241 tcacctcata cagctcatcg ccataactta tagttttcaa cagctcttcc ttttcccgaa
      301 tgaaaaatac ttgctgcatt ccaaggatca ggtcataggc ctctcgaaag aggtcgataa
      361 gcttattacg aacataaatc ataaactcct caaagtgctg aagccagaaa taagccgcat
      421 tacttatttg tgaattgtga gaacaaacaa gccctttagc aagctcgacc tcttcctccc
      481 gaaaagtaaa gttacttttc ttaaaccgac tttgttcaaa aatgctttga tgcatttcta
      541 tccatacaga aacaaaaccg agggattcaa attcttcgtt taacaaatgt aaactttcga
      601 agattttctg aatttcgatc ccgatgtcat atgacagtgg acattctttt atagaataaa
      661 cataaccggg taggaagtga gataaatgct cagttcgcaa atataaactg aatttaagtt
      721 ctgtcagtgg ttgtttcaaa gataacttag atgattcgct tatatttact ggttcaacgc
      781 catttctaat caaaatagct tttaccaggt ccgtagctaa cttcatacag cctccattcg
      841 ttattgaggc ctccctgatg caggttggcc atcagggaaa atatgtaaat caattagcaa
      901 tcaatcagac cgttgccatg acattgtcaa tgtatctgac aagggatggg cggtcgtcct
      961 gtaaggcaaa acggcgaagg tcatcaagat tatacgcagg aagataatca gcgtaacctc
     1021 gaatacgaag gtcctcaacc acaactttta tatactcaaa tcgtccaaga ttccctttta
     1081 atgggggttt tcctggacta gacagcatat cgtctgcttg ctcccatgtg atttctttag
     1141 cttcaccact atgtagatca agatggacat atatgaatga cttataatct ccacggcaac
     1201 gaagataata tttcggatag aaatatttgt cacaacgcaa caaacacgtt cctgaaggac
     1261 gaactacgta caacaggttt ctgtcatttt ctaatatgct atgtttcaaa atcgttatat
     1321 ctatctcat
//
LOCUS       DAPGOF010000347         1312 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.355, whole genome shotgun sequence.
ACCESSION   DAPGOF010000347 DAPGOF010000000
VERSION     DAPGOF010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.355"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(32..736)
                     /locus_tag="RHV13_004752"
     CDS             complement(32..736)
                     /locus_tag="RHV13_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HDV1244175.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEMRLVSRVFEM"
     gene            800..1312
                     /locus_tag="RHV13_004753"
                     /pseudo
     CDS             800..1312
                     /locus_tag="RHV13_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000790.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT          318 a          357 c          322 g          315 t
ORIGIN      
        1 atgaggcagc cttttgtctt attcaaaggc cttacatttc aaaaactctg cttaccaggc
       61 gcatttcgcc caggggatca ccataataaa atgctgaggc ctggcctttg cgtagtgcac
      121 gcatcacctc aatacctttg atggtggcgt aagccgtctt catggattta aatcccagcg
      181 tggcgccgat tatccgtttc agtttgccat gatcgcattc aatcacgttg ttccggtact
      241 taatctgtcg gtgttcaacg tcagacgggc accggccttc gcgtttgagc agagcaagcg
      301 cgcgaccata ggcgggcgct ttatccgtgt tgatgaatcg cgggatctgc cacttcttca
      361 cgttgttgag gattttaccc agaaaccggt atgcagcttt gctgttacga cgggaggaga
      421 gataaaaatc gacagtgcgg ccccggctgt cgacggcccg gtacagatac gcccagcggc
      481 cattgacctt cacgtaggtt tcatccatgt gccacgggca aagatcggaa gggttacgcc
      541 agtaccagcg cagccgtttt tccatttcag gcgcataacg ctgaacccag cggtaaatcg
      601 tggagtgatc gacattcact ccgcgttcag ccagcatctc ctgcagctca cggtaactga
      661 tgccgtattt gcagtaccag cgtacggccc acagaatgat gtcacgctga aaatgccggc
      721 ctttgaatgg gttcatgtgc agctccatca gcaaaagggg atgataagtt tatcaccacc
      781 gactatttgc aacagtgccg cctttgttaa ggcagctata actgcactta tcaacggtgc
      841 gcctggcaca ctggatacgc tgaaagaaat agcggctgcg atcaataacg acccgaatta
      901 cagcacaact atcaacaatg ccttggctct caaagcgcct ttggcaagcc ctgcattaac
      961 gggtgtccct actgcgccta cggctgcaca gggcacaaac aatacgcaga tcgctacgac
     1021 tgcttacgta cgggctgcta tctctgcatt ggtcggatca tcacctgaag ctcttgatac
     1081 cctgaatgag cttgcagcag cactgggcaa tgacccgaac tttgcgacaa caatgacaaa
     1141 tgcgctggca gggaaacagc cacttgatgc aactttaacc gcgcttgctg gtcttgcgac
     1201 aggcgcaaat aaattgccgt actttaccgg tacagacact gtttctcaga ctgacttaac
     1261 gtcagtcggt cgcgatattc tggccaaaac aagcgttctt gctgttatcc aa
//
LOCUS       DAPGOF010000348         1292 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.356, whole genome shotgun sequence.
ACCESSION   DAPGOF010000348 DAPGOF010000000
VERSION     DAPGOF010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.356"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            163..765
                     /locus_tag="RHV13_004754"
     CDS             163..765
                     /locus_tag="RHV13_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDV1244176.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKDKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(804..1229)
                     /gene="nudI"
                     /locus_tag="RHV13_004755"
     CDS             complement(804..1229)
                     /gene="nudI"
                     /locus_tag="RHV13_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HDV1244177.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
BASE COUNT          357 a          288 c          270 g          377 t
ORIGIN      
        1 tttatatttt ttcttattta ggctttgcat ttggcaaaat tttgaggcat tttgccgaca
       61 tcgtaggatt tttaatatta caccaactgc taattatcgc cagaaatgtc gctcaatttc
      121 acggtaattg tctggttgcg cttgtctata ggtggagttt acgtgttagc tttttgccgc
      181 tcatcattga agtcaaaaaa atatttcatc attttactgg cgctcgctgc aattgccgga
      241 ctgggtactc atgccgcctg gagtagcaat ggtttgccac gtatcgacaa taaaacattg
      301 gccagactgg cacagcagca cccggttgtc gttttgtttc gccatgctga acgttgcgat
      361 cgttcaacca atcaatgctt gtcagataaa acaggtatta cggtcaaagg tacccaggat
      421 gcccgagaac tgggcaacgc ttttagtgct gatatccctg atttcgatct ctattccagt
      481 aataccgtcc ggaccattca gtcggcgacc tggttttcag cgggtaaaaa gctcacggta
      541 gataaacgac ttcttcaatg cggtaatgag atctatagtg caattaaaga cttacaaagc
      601 aaagcgcctg ataaaaatat cgttattttc acccataatc attgcctgac atatattgct
      661 aaagataagc gtgatgcgac atttaaacct gattatctgg atggtttagt catgcatgtg
      721 gaaaaaggca aagtttatct ggatggggaa ttcgttaacc actaaatttt caatctgaca
      781 gccagtgatg gctgtcatca ttgttaaaga agacctttca aacgtaacgt ttttcgggtg
      841 gcaacattca aatcataatg caccagatct tcaggtttta cccacgcgta gtcctgaaac
      901 tcttcgttta ttttcacttc tcggttggca gaaacgcagt caaaaatcag gtaaatcata
      961 taaatctctt ccttgcgacc atctgcatac gtcttggtgc gaatatcatc gctgaaggtc
     1021 cacggcgtga tttctgtcaa aagcagctgt tctcccaatt cttcgcgaat ttcgcggcgt
     1081 agtgcctctt caattcgttc gccgggctcc acgccgccac ccgaaagcgc ccattgaccg
     1141 gggaaaacgc cgcgatcgtc ggccatttta cacagcaaat aagcaccatc attttgaatc
     1201 aaagggcata caatagtccg ttgtcgcaca ggggtattcc ttataaataa aaagaaacca
     1261 tgagaatacg gtaactcaga aatatgtgaa ag
//
LOCUS       DAPGOF010000349         1281 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.357, whole genome shotgun sequence.
ACCESSION   DAPGOF010000349 DAPGOF010000000
VERSION     DAPGOF010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.357"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            28..1239
                     /gene="bluF"
                     /locus_tag="RHV13_004756"
     CDS             28..1239
                     /gene="bluF"
                     /locus_tag="RHV13_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HDV1244178.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQCDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDFYTLSPNISDHFAFHPIVDPLSRRIIAFEAIEQKNEDSPSAIAVGQRKDGEIYKAD
                     LKSKALAFTMAHALELGDKMISINLLPMTLVNEPDAVSFLLNEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
BASE COUNT          367 a          270 c          277 g          367 t
ORIGIN      
        1 cacctctccc attaccctgg acagttaatg cttaccaccc ttatttatcg tagccatata
       61 cgtgacgacg aacctgtcaa aaaaatcgaa gaaatggttt cgatagcaaa tcgcaggaac
      121 atgcagtgtg acgtaacagg gatcttactg tttaatggtt ctcatttttt ccagcttctg
      181 gaaggtccgg aagaacaggt taaaatgata tatcgggcta tatgccagga tcctcggcac
      241 tataatattg ttgaactgct gtgcgattac gcgcctgctc gccgttttgg caaagcggga
      301 atggaattat ttgatttgcg cttgcacgag cgagatgacg ttttacaggc cgtattcgac
      361 aaaggcacat caaaatttca gctaacttat gatgacagag cgctacaatt ttttcgtact
      421 tttgtccttg caaccgaaca atcaacctat ttcgagatcc ctgccgaaga ttcctggctt
      481 tttatcgctg acggatctga taaagaactt gatttctata ccctttcacc aaatataagc
      541 gaccactttg cctttcatcc aattgtcgat cccttatcgc ggcggataat cgcttttgaa
      601 gccattgagc aaaaaaatga agatagtcca tcagctatag cggttgggca gcgtaaagac
      661 ggggaaatct acaaagcgga tctcaaaagt aaggcgcttg cattcacgat ggcacacgca
      721 cttgagctcg gtgataaaat gatttcaatc aatctattac ctatgaccct ggttaacgaa
      781 cctgacgcag tctctttttt acttaatgaa ataaaggcca atgctctggt gcctgaacaa
      841 atcatcgttg aatttactga aagtgaagtc atatctcggt ttgatgagtt tgccgaagcg
      901 attaaatcgc taaaggctgc tggtatcagt gtagcaattg atcattttgg cgcaggtttt
      961 gctggtttgt tactcctgtc acgcttccag cctgacagaa ttaaaatcag tcaggaattg
     1021 attaccaatg ttcataaaag tgggccacgg caggcaatta ttcaggcgat cataaaatgc
     1081 tgtacatcac ttgaaattca agtcagtgct atgggcgtgg caacaccaga agagtggatg
     1141 tggcttgaat ccgcaggaat tgagatgttt cagggagatc tgtttgctaa agctaaattg
     1201 aatggtatcc cttccatagc gtggccagag aaaaaataat tttcagcaca ttctttcaca
     1261 tgatttcagt aaatcatcgt g
//
LOCUS       DAPGOF010000350         1272 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.358, whole genome shotgun sequence.
ACCESSION   DAPGOF010000350 DAPGOF010000000
VERSION     DAPGOF010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.358"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1159
                     /locus_tag="RHV13_004757"
     CDS             <1..1159
                     /locus_tag="RHV13_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HDV1244179.1"
                     /translation="VSTDELSSYPEGALVTFDRPFEIIANSYEEYRTPVYAVSFSKDE
                     FSEDIKTFESLHDASEYKNKMLQEHGLNQDDILITPVTREEIAFKGIKDAVNDANMAV
                     MEQAGDSSRESPEEILASISANEHMISGLENFLVKDRSQFSSCNGDIVVEAEITRGEG
                     GLYRLAVAGKHGLERGDAVARVDVTEQQFAAITGKTPSEVLTGDQTSARVPVITGIHF
                     STRAIENVNKLEQQKDYVYFSTHEGLNAEIKDFSSLKDAIEWGRVECELHDLNKRDTV
                     IYRVESEHISQGIDAVMKNAERVERHEIEKAQGRDCTPEDGKILEAIDRFEDKFRGEG
                     LKFEREKAESDLLNHGFTREMAEDALGKQFVQAREDHQESQQQRDDSQDMH"
BASE COUNT          398 a          233 c          326 g          315 t
ORIGIN      
        1 tgtctcaacc gatgaattat cctcttatcc agagggggca ttagtaacgt ttgataggcc
       61 atttgaaata attgccaatt catatgaaga ataccgtact ccagtgtatg cggtttcttt
      121 ttcgaaggat gagtttagtg aagatattaa gacttttgag tcattgcacg acgcatctga
      181 atataaaaat aagatgcttc aggagcacgg gcttaaccag gatgatatct taatcacacc
      241 tgtaacaaga gaagaaatcg cctttaaagg aattaaggac gctgttaatg acgctaatat
      301 ggcagtgatg gaacaggcgg gggattcgtc cagagaatca ccagaagaga tactcgccag
      361 tatttcagct aacgagcata tgatctccgg gcttgaaaac ttcctggtta aagaccgttc
      421 tcagttcagt tcctgcaatg gggatatcgt tgtggaggcg gaaattacca ggggggaagg
      481 tggtctttat cgccttgcgg tcgctggtaa gcatggctta gagcgtggtg atgcagttgc
      541 cagagtggat gttacggagc agcaatttgc cgcgatcact ggaaaaacac cttctgaagt
      601 tttaacgggg gaccaaacat cggctcgtgt tccggtaatt acaggtatcc atttcagtac
      661 aagggctatt gaaaatgtga ataaactgga acaacagaag gattatgtct atttctcaac
      721 acatgaaggt ctgaatgctg aaataaaaga tttcagctct ctcaaggatg ctattgagtg
      781 gggccgggtt gaatgcgagc tacatgatct caataagcgc gatactgtca tttatcgcgt
      841 cgagtctgaa catatttctc aggggattga cgctgtaatg aaaaatgctg aacgcgtgga
      901 gcgtcatgaa attgagaaag cacagggacg ggattgcacg ccggaagacg gcaagattct
      961 tgaggcaatt gatcgctttg aggacaagtt cagaggggaa ggactgaagt tcgaaaggga
     1021 gaaggccgaa tccgatttgc ttaatcacgg attcactcgt gaaatggcag aagatgctct
     1081 tggaaaacaa tttgtccagg ccagagaaga tcatcaggaa tcgcagcagc agcgtgacga
     1141 ttcacaggac atgcactaat taagaaaaaa gcccggtttt cgggcttttt aagaaaaaaa
     1201 gtcaacccca aacagaaata atagctacaa aaatggacag taataatgca ataaaagtaa
     1261 tgttgagcca ta
//
LOCUS       DAPGOF010000351         1253 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.359, whole genome shotgun sequence.
ACCESSION   DAPGOF010000351 DAPGOF010000000
VERSION     DAPGOF010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.359"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..488
                     /locus_tag="RHV13_004758"
     CDS             <1..488
                     /locus_tag="RHV13_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HDV1244180.1"
                     /translation="HDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQHLSETKN
                     QIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYVFEQFEIA
                     CYTSLLAAAKNAGDTASIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSETDGVEAK
                     K"
     gene            complement(548..1186)
                     /gene="ompW"
                     /locus_tag="RHV13_004759"
     CDS             complement(548..1186)
                     /gene="ompW"
                     /locus_tag="RHV13_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HDV1244181.1"
                     /translation="MKKLTVAALAVTTLLSGSAYAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
BASE COUNT          357 a          305 c          268 g          323 t
ORIGIN      
        1 atcatgactg gttacgtgac gcccatgcaa tggaaaagca agccgaatct atgcttgaat
       61 ccatggccag ccgtatagat aattatcctg aactacgtgc tcgtattgaa caacatctta
      121 gtgaaaccaa aaaccagatt gttcaactgg aaactattct tgatcgtaat gacatttcac
      181 gttcagtcat taaagattcc atgagtaaaa tggctgcgct tgggcagtca atcggtggta
      241 tattcccttc tgatgaaata gtcaaaggct ctattagcgg atatgtcttc gagcaatttg
      301 aaatcgcctg ttacacctct ctattagcag cagcaaaaaa tgccggtgat acagcctcaa
      361 ttccgatcat cgaagcgatt ttaaatgagg aaaagcaaat ggccgactgg ctgattcagc
      421 atattccgca aacaactgag aaatttttaa ttcgctctga aactgatggc gtagaagcga
      481 agaaataata ccctttatac catgtcctta ttgaccccgt atattacggg gtcgtttttg
      541 tgcggaatta aaaacgatat cctgctgaga acataaacac ccacggatcg aggcgtacgc
      601 tatcgtgttg ctgtgcaccg cctagcttat aattggcggt ggtatcgata tccatgtacc
      661 acactgacat gttaaccaac cagtcacggt taatcagata atcaaccccc acctgcccgg
      721 cagctcccca ggaatctttc agactgagat cggaaagccc cgcctctttg ccatgatcgt
      781 taaatccatt atcaaagaag gtggtgtagt taatacctgc cccaacataa ggacggaatt
      841 tgctgctggc atcaccaaaa taccactgcg ccatcagtgt tggcggcaga tgatgaacgg
      901 ttgcaatatc gccggtcgcc cgggtgccga ttttatggcg gaacggcgtc gctgccagta
      961 attccacacc aatgttgtcg gtcgccatat aagtaaacgt caggcccagt tgagtgttat
     1021 tggtcacgct gaatccaccc agacttccta acgtaccacc agcaccttct gttggacgta
     1081 cggttgcaga acctgcacgc ataaaaaatt cgcctgcttc atgcgcatag gcactgccag
     1141 agagaagagt tgttactgcc aaagccgcca ctgttaactt tttcatatcc gctccgtcgt
     1201 tatggttata aaaacgtgac gaatatacct acattggagt aacaaatgat cct
//
LOCUS       DAPGOF010000352         1241 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.360, whole genome shotgun sequence.
ACCESSION   DAPGOF010000352 DAPGOF010000000
VERSION     DAPGOF010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.360"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>1241
                     /locus_tag="RHV13_004760"
     rRNA            <1..>1241
                     /locus_tag="RHV13_004760"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          313 a          288 c          391 g          249 t
ORIGIN      
        1 ctgagaggat gaccagccac actggaactg agacacggtc cagactccta cgggaggcag
       61 cagtggggaa tattgcacaa tgggcgcaag cctgatgcag ccatgccgcg tgtatgaaga
      121 aggccttcgg gttgtaaagt actttcagcg gggaggaagg gagtaaagtt aatacctttg
      181 ctcattgacg ttacccgcag aagaagcacc ggctaactcc gtgccagcag ccgcggtaat
      241 acggagggtg caagcgttaa tcggaattac tgggcgtaaa gcgcacgcag gcggtttgtt
      301 aagtcagatg tgaaatcccc gggctcaacc tgggaactgc atctgatact ggcaagcttg
      361 agtctcgtag aggggggtag aattccaggt gtagcggtga aatgcgtaga gatctggagg
      421 aataccggtg gcgaaggcgg ccccctggac aaagactgac gctcaggtgc gaaagcgtgg
      481 ggagcaaaca ggattagata ccctggtagt ccacgccgta aacgatgtcg acttggaggt
      541 tgtgcccttg aggcgtggct tccggagcta acgcgttaag tcgaccgcct ggggagtacg
      601 gccgcaaggt taaaactcaa atgaattgac gggggcccgc acaagcggtg gagcatgtgg
      661 tttaattcga tgcaacgcga agaaccttac ctggtcttga catccacaga accttgtaga
      721 gatacgaggg tgccttcggg aactgtgaga caggtgctgc atggctgtcg tcagctcgtg
      781 ttgtgaaatg ttgggttaag tcccgcaacg agcgcaaccc ttatcctttg ttgccagcgg
      841 tccggccggg aactcaaagg agactgccag tgataaactg gaggaaggtg gggatgacgt
      901 caagtcatca tggcccttac gaccagggct acacacgtgc tacaatggcg catacaaaga
      961 gaagcgacct cgcgagagca agcggacctc ataaagtgcg tcgtagtccg gattggagtc
     1021 tgcaactcga ctccatgaag tcggaatcgc tagtaatcgt ggatcagaat gccacggtga
     1081 atacgttccc gggccttgta cacaccgccc gtcacaccat gggagtgggt tgcaaaagaa
     1141 gtaggtagct taaccttcgg gagggcgctt accactttgt gattcatgac tggggtgaag
     1201 tcgtaacaag gtaaccgtag gggaacctgc ggttggatca c
//
LOCUS       DAPGOF010000353         1231 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.361, whole genome shotgun sequence.
ACCESSION   DAPGOF010000353 DAPGOF010000000
VERSION     DAPGOF010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.361"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            57..>1231
                     /gene="ompC"
                     /locus_tag="RHV13_004761"
     CDS             57..>1231
                     /gene="ompC"
                     /locus_tag="RHV13_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HDV1244182.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEVYNKDGNKLDLYGKVVGLHYF
                     SDDSGSDGDMSYARIGFKGETQIADQFTGYGQWEFNIGANGTESDKGNTATRLAFAGL
                     GFGQNGTFDYGRNYGVVYDVEAWTDMLPEFGGDTYAGADNFMNGRANGVATYRNNGFF
                     GQVDGLNFALQYQSNNENGGPFGQEGSGKGKGRDIAQENGDGFGMSTSYDFDFGLSLG
                     AAYSNSDRSDRQVGVGLNDRNHSNRNAGGETAEAWTVGAKYDANNVYLAAMYAETRNM
                     TPYGGGEFDNGESRSAIANKTQNFEVVAQYQFDFGLRPSIAYLQSKGKDLGGWAHDGN
                     GDPRYTNKDLVKYVDIGATYYFNKNMSTYVDYKINLLDNDDDFYEANGIATDDIVAVG
                     "
BASE COUNT          342 a          242 c          312 g          335 t
ORIGIN      
        1 ggataacggc acttattgat atattcatga agattataat cacaagggaa tacataatga
       61 aaagaaaagt tctggcaatg ctggtcccgg cgttattagt tgctggcgca gcaaatgcgg
      121 ctgaagtcta taataaagat ggtaataaac tggatttgta cggaaaagta gtgggtctgc
      181 actacttctc tgatgattct ggcagcgatg gcgacatgtc atatgcccgt atcggtttca
      241 aaggtgaaac tcagatcgct gatcaattca ctggttacgg tcagtgggaa tttaacattg
      301 gcgcaaacgg tactgaaagc gacaaaggta atactgcaac gcgtctggca tttgcaggtt
      361 taggctttgg tcagaatggt actttcgact atggtcgtaa ctacggtgtc gtatatgacg
      421 tagaagcatg gaccgatatg ctgccagaat ttggtggcga tacctatgct ggcgctgaca
      481 acttcatgaa cggtcgtgct aatggtgttg caacctaccg taacaatggc ttctttggtc
      541 aggtcgatgg cctgaacttt gctctgcaat atcagagtaa taacgaaaac ggtggtccat
      601 tcggtcagga aggttctggt aagggtaagg gtcgggacat tgctcaagaa aatggtgatg
      661 gtttcggtat gtccacttcc tatgacttcg acttcggttt aagtctgggt gctgcatatt
      721 caaactctga tcgttcagat cgtcaggttg gagttggttt aaatgatcgt aaccatagca
      781 acagaaatgc tggtggtgaa accgctgaag catggactgt tggtgcaaaa tatgatgcta
      841 ataatgtgta tttagcagct atgtacgctg aaactcgcaa tatgacccca tatggtggtg
      901 gcgagtttga taatggtgaa tcaagatcag ccatcgctaa caaaacccaa aactttgaag
      961 ttgttgctca gtatcagttc gactttggcc tgcgcccgtc catcgcttac ctgcaatcta
     1021 aaggtaagga tttaggcggt tgggctcatg acggcaacgg cgacccacgt tacaccaata
     1081 aagatctggt taaatacgtt gatattggtg cgacctacta cttcaacaaa aacatgtcca
     1141 cctatgttga ttacaaaatc aacctgctgg acaacgacga tgacttctac gaagcaaacg
     1201 gcatcgctac cgatgatatc gttgctgtgg g
//
LOCUS       DAPGOF010000354         1218 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.362, whole genome shotgun sequence.
ACCESSION   DAPGOF010000354 DAPGOF010000000
VERSION     DAPGOF010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.362"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            145..822
                     /locus_tag="RHV13_004762"
     CDS             145..822
                     /locus_tag="RHV13_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HDV1244183.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNHLAHVSRRKVERDLQGVVEVLDNQGYDVIILMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEELLTVQAQKWQILQKPPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFNQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            894..1178
                     /gene="ppnP"
                     /locus_tag="RHV13_004763"
     CDS             894..1178
                     /gene="ppnP"
                     /locus_tag="RHV13_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HDV1244184.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT          305 a          271 c          316 g          326 t
ORIGIN      
        1 actggaatca tttggaatct ttacattatg ccgtgcacgt ctgctgctac gctttttgtc
       61 atttgtagca caagtaagtg tcagcagtgg tgcttcacac ttgcccggta attaacgacg
      121 aaagaaaagt aaggtggatg aacaatgagt gcgtcgttgg cgatcctgac catcggcatt
      181 gtacctatgc aggaagtttt gccgctcctg acggaataca ttgacgaaga taatatttcc
      241 catcatagcc tgctggggaa gttaagccgc gaagaagtga tggcggagta cgcgccagaa
      301 gcaggcgaag acacaattct cacattatta aatgacaacc atctggccca tgtttcgcgt
      361 cgcaaagtag agcgtgacct gcaaggtgtg gttgaagtgc tcgataatca gggctacgac
      421 gtcattatat taatgagtac ggcaaacatt agtagtatga ctgcgcgtaa tacgatcttt
      481 cttgagccgt cgcggatatt gccaccgctg gtttcctcta ttgttgaaga tcatcaggtg
      541 ggggttatcg taccggttga ggagttgctg accgttcagg cgcaaaaatg gcaaattttg
      601 cagaaaccgc cggtattttc attgggtaac cccattcatg attcagaaca aaaaatcatt
      661 gatgccggga aagaattact ggcgaaaggc gcagatgtca tcatgctgga ttgtttggga
      721 tttaaccagc gtcatcgcga tttactgcaa aaacagctcg atgttcctgt cttgctgtct
      781 aacgtattga ttgcacggct agctgcggaa ttactggtgt aattttgcgt gacagccagc
      841 gcctctggcc cctatagtga agtagatgtt caactaccag acagggccag tttatgcttc
      901 aaagtaatga gtacttttcc ggcaaagtga aatcgatcgg cttttccagc agcagcactg
      961 gtcgcgccag cgtgggtgtt atggttgaag gcgaatacac cttcagcacc gctgagccgg
     1021 aagaaatgac ggtaatcagt ggcgcgctga atgtgttact gcctgacgcg accgactggc
     1081 aagtgtatga agccggttcg gtgtttaatg ttcccggtca cagtgagttt catctgcaag
     1141 ttgccgaacc cacctcttat ctgtgccgct atctgtaatt cctcgccttc cccttgaacg
     1201 ggagggcatt tttctgaa
//
LOCUS       DAPGOF010000355         1212 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.363, whole genome shotgun sequence.
ACCESSION   DAPGOF010000355 DAPGOF010000000
VERSION     DAPGOF010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.363"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            5..1042
                     /locus_tag="RHV13_004764"
     CDS             5..1042
                     /locus_tag="RHV13_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stbA family protein"
                     /protein_id="HDV1244185.1"
                     /translation="MAELLKSESPVSQSLQDITEALKIVVDDGSKAAKLVCLNERNEL
                     VPLLTQNSFVPDFRVRHEGLNPFNYLIDGLERFSHHSESSNSLESTDVSHQYDEISRL
                     NVHHALHASGLVPQDVHLFVTLPLSQFYTALGETNIENIQRKKDNLMKPVERYLDGKR
                     YSFNVLSVTVFPESLPAVTRADEIEDIASFESSLVIDLGGTTLDVASITGQLEQISKV
                     KGFDRIGCSIVYDEISRYLESEKLNTSNAYIHHLVDNRHDKSALKVAEDKRDGVFDAV
                     NSAVQKLQSKVIRAVTQVEERPHNVFLVGGGSYLIETAIRKHFETAKVIMVDNPQFAL
                     SLAIADTIYSE"
BASE COUNT          358 a          215 c          252 g          387 t
ORIGIN      
        1 ttttatggct gaactattaa aatctgaaag tcctgtttca caatcattgc aggatatcac
       61 tgaagcttta aaaatcgttg tcgatgatgg aagtaaagct gcaaaactgg tttgtcttaa
      121 tgaaagaaac gaattagttc ccttgctcac acaaaatagt tttgttccag atttccgtgt
      181 tcgccatgag ggtctcaatc cttttaacta tcttattgat gggctggaaa ggttttcgca
      241 tcacagcgaa tctagtaact cactggaatc gactgatgta tctcaccaat acgatgaaat
      301 tagccggtta aatgttcacc atgcacttca tgcttctggt ttagttcctc aggatgttca
      361 cctctttgtt actttacctc ttagccagtt ctatactgct ttaggggaaa cgaatattga
      421 aaatattcag agaaaaaaag ataacctgat gaaaccagtt gagcgttatc ttgacggtaa
      481 acgctattct ttcaacgttt tgtctgtgac tgttttccct gaatctttac cagctgtgac
      541 ccgggcagat gaaattgagg atattgcttc atttgaatca agtttggtaa ttgatcttgg
      601 tggtactact cttgatgttg caagtattac tgggcaatta gaacagattt cgaaagtaaa
      661 aggttttgac cgtattggtt gttctattgt ttacgatgag atcagcaggt atcttgaatc
      721 tgagaaactg aatacgagta atgcttacat tcatcatctt gttgataatc gtcatgataa
      781 atcagctttg aaggttgcag aggacaagcg tgacggtgtt tttgatgcgg taaactctgc
      841 ggtacagaag ttgcaaagta aagtaatcag agcggttaca caagtcgaag agaggcctca
      901 taatgttttc cttgttgggg gaggttcata tctcatcgaa acagctattc gtaaacattt
      961 tgaaacggct aaagttatca tggtagacaa tccgcagttc gcattatctt tggcaattgc
     1021 tgatactatt tattcagaat aatgagctag aacatggcta aaaaccctat ctcaaataag
     1081 gcagataacg accggattca gatccggtct ttctggatat ccgaaagaaa agcaccctat
     1141 gtttatagtt tcttgaaaaa aacagaactt tctcataggg gtgaccaact ggatttaatt
     1201 aggtcggcta tt
//
LOCUS       DAPGOF010000356         1208 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.364, whole genome shotgun sequence.
ACCESSION   DAPGOF010000356 DAPGOF010000000
VERSION     DAPGOF010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.364"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..1153)
                     /locus_tag="RHV13_004765"
     CDS             complement(<1..1153)
                     /locus_tag="RHV13_004765"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDV1244186.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKD"
BASE COUNT          284 a          325 c          316 g          283 t
ORIGIN      
        1 catctttcag tttgaagcaa accgccggga tgccttcgtc cgggcgcccc gtacagatga
       61 actcatacgg ccccagtttg gcgatttcat ccgccagata ggcagcgacc tggtaggaag
      121 cattctgtac tttggtatag ccttcacgac caaggcgcag gaattcatag tactgtgcaa
      181 tcacctgacc tgccgggcgg gagaagttga tggcaaaagt accaatctga ccacccaggt
      241 agtcaacgtt gaacaccagt tcctgcggca gcgcttcttc gtcacgccag ataacccagc
      301 cgcagcccag cggagccaga ccgaatttat ggcctgaagc actgatcgat ttcacacgcg
      361 gcaggcggaa gtcccagacg atatccgggg cgacgaacgg tgccagaaag ccaccgctgg
      421 cggcgtcgat gtgcatgtcg atgtcgatac cggtgtcggc ctggaattta tccagcgcat
      481 cgtgcagcgg ttgcgggaac tcatagttac cggtgtaggt cacgccgaaa gtcggcacca
      541 cgccgatggt gttttcgtcg caggcttcaa tcatgcgttt cgggtccata aacaactgac
      601 cggggcgcat agggatctca cgcagctcca catcccagta gcgggcgaat ttatgccagc
      661 agatttggac cggaccgcac accaggtttg gtttatccgt tggtttgcct gcagcttcca
      721 tacgcttgcg ccaacgccat ttcatcgcca tcccgccgag catacaggcc tcggaagaac
      781 caatggtgtt ggtgccaacg gcctgaccat ttttcggcgc aggcgcatgc cacagatcgg
      841 caaccatgtt tacgcaacgc aggtcgatgg ctgcggattg cggatattct tctttgtcga
      901 tccagttttt attgatcgac aaatccatca atttatggac gttttcgtcg tcccaggtct
      961 ggcagaaagt ggccaggttc tgacgagcgt tgccatcaag atataattca tcattgataa
     1021 tctgaaatgc gacatcatcg cgcatttcgt gcagcggaaa tcgttttgac tccgcgatag
     1081 tagaaatggc ctttgcgcca aaacgtgaat cgagtagttc tgagcggaaa tccgttaaca
     1141 gcttctggtc catttcgaac tccttaaatt tatttgaagg caataaaaaa gtaggattta
     1201 tccgcaat
//
LOCUS       DAPGOF010000357         1205 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.365, whole genome shotgun sequence.
ACCESSION   DAPGOF010000357 DAPGOF010000000
VERSION     DAPGOF010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.365"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..185
                     /locus_tag="RHV13_004766"
     CDS             <1..185
                     /locus_tag="RHV13_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HDV1244187.1"
                     /translation="LSDSELNKINNYVDEYYKQWGKQYVRFNVNLKNQDTNNSSFSYG
                     DNRFEKSQGSKWTFQE"
     gene            198..437
                     /locus_tag="RHV13_004767"
     CDS             198..437
                     /locus_tag="RHV13_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
                     /protein_id="HDV1244188.1"
                     /translation="MADWNGYIMDISKQFDQGVDDLNQQVEKALEDLATNPSDPKFLA
                     EYQSALAEYTLYRNAQSNVVKAYKDLDSAIIQNFR"
     gene            457..789
                     /gene="sctI"
                     /locus_tag="RHV13_004768"
     CDS             457..789
                     /gene="sctI"
                     /locus_tag="RHV13_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HDV1244189.1"
                     /translation="MSVSNMPPIDRVEQSTAHEIQQAKVIDLNDRVLNLDNPDDKMIS
                     AFANYAVQTENWQQNALQALRSDKKGLTPEKLLVLQDHVLNYNVEVSLVGTLARKIVA
                     AVETLTRS"
     gene            786..>1205
                     /gene="sctJ"
                     /locus_tag="RHV13_004769"
     CDS             786..>1205
                     /gene="sctJ"
                     /locus_tag="RHV13_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion inner membrane ring
                     lipoprotein SctJ"
                     /protein_id="HDV1244190.1"
                     /translation="MKYISLLLFILLLCGCKQQELLNHLDQQQANDVLAVLQRHNINA
                     EKKDQGKTGFSIFVEPTDFASAVDWLKIYNLPGKPDIQISQMFPADALVSSPRAEKAR
                     LYSAIEQRLEQSLKIMDGIVSSRVHVSYDVDTGDSGKT"
BASE COUNT          401 a          203 c          256 g          345 t
ORIGIN      
        1 cgttaagcga cagtgaatta aacaaaataa ataactatgt tgacgaatat tataaacaat
       61 ggggtaaaca atatgtaaga tttaatgtga atttgaaaaa tcaggataca aataatagtt
      121 catttagcta tggagataac cgattcgaga agtctcaagg tagcaagtgg acgtttcagg
      181 aataattggg aggacttatg gcagattgga atggttatat tatggatatc agcaaacaat
      241 ttgatcaggg cgttgatgat ctgaaccagc aagttgaaaa agcgttggag gatttagcaa
      301 ccaatccctc cgacccgaaa ttccttgccg aatatcaaag tgcattagct gagtatacat
      361 tgtatcgaaa tgcgcaatct aacgttgtaa aagcgtataa agatcttgat tcggcaatca
      421 tacaaaactt ccgctaaact ggataaggat tttattatgt cagtaagcaa tatgccccct
      481 attgatagag ttgagcaatc aactgctcat gaaatacagc aagcgaaagt gattgattta
      541 aatgaccggg ttctcaatct tgacaacccg gatgataaaa tgatctccgc ttttgccaac
      601 tatgctgtac aaactgaaaa ttggcagcaa aatgcgttac aagcattaag gtctgataaa
      661 aaagggttaa ctcctgaaaa attattagtc ttgcaggatc atgtgcttaa ttataatgtc
      721 gaggtttcat tggtgggaac attagcaaga aagattgtcg ctgcagttga gacgttgacc
      781 cgctcatgaa atatatatca ttattattgt ttatcctgct gttgtgcggc tgcaagcagc
      841 aggaattatt gaaccatctt gatcaacagc aagctaatga tgtccttgct gttttacaaa
      901 gacataatat taatgctgaa aagaaggatc aaggcaaaac aggattttca atatttgtcg
      961 aaccaacgga ttttgcttct gcggtggatt ggctaaaaat ctacaatctc ccgggtaagc
     1021 ctgatattca aatatcgcag atgttccctg cagatgcact cgtttcatct ccacgagcgg
     1081 aaaaagcaag gctatattct gctattgagc agcggttaga gcaatctctc aaaattatgg
     1141 atggcattgt ttcttcgaga gtacatgtta gttacgatgt tgatactggc gatagtggta
     1201 aaact
//
LOCUS       DAPGOF010000358         1187 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.366, whole genome shotgun sequence.
ACCESSION   DAPGOF010000358 DAPGOF010000000
VERSION     DAPGOF010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.366"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            39..>1187
                     /locus_tag="RHV13_004770"
     rRNA            39..>1187
                     /locus_tag="RHV13_004770"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          338 a          255 c          363 g          231 t
ORIGIN      
        1 cacgatgatg aatcgaaaga aacatcttcg ggttgtgagg ttaagcgact aagcgtacac
       61 ggtggatgcc ctggcagtca gaggcgatga aggacgtgct aatctgcgat aagcgtcggt
      121 aaggtgatat gaaccgttat aaccggcgat ttccgaatgg ggaaacccag tgtgtttcga
      181 cacactatca ttaactgaat ccataggtta atgaggcgaa ccgggggaac tgaaacatct
      241 aagtaccccg aggaaaagaa atcaaccgag attcccccag tagcggcgag cgaacgggga
      301 ggagcccaga gcctgaatca gtgtgtgtgt tagtggaagc gtctggaaag gcgcgcgata
      361 cagggtgaca gccccgtaca caaaaatgca catgctgtga gctcgatgag tagggcggga
      421 cacgtggtat cctgtctgaa tatgggggga ccatcctcca aggctaaata ctcctgactg
      481 accgatagtg aaccagtacc gtgagggaaa ggcgaaaaga accccggcga ggggagtgaa
      541 aaagaacctg aaaccgtgta cgtacaagca gtgggagcct cttttatggg gtgactgcgt
      601 accttttgta taatgggtca gcgacttata ttctgtagca aggttaaccg aataggggag
      661 ccgaagggaa accgagtctt aactgggcgt taagttgcag ggtatagacc cgaaacccgg
      721 tgatctagcc atgggcaggt tgaaggttgg gtaacactaa ctggaggacc gaaccgacta
      781 atgttgaaaa attagcggat gacttgtggc tgggggtgaa aggccaatca aaccgggaga
      841 tagctggttc tccccgaaag ctatttaggt agcgcctcgt gaattcatct ccgggggtag
      901 agcactgttt cggcaagggg gtcatcccga cttaccaacc cgatgcaaac tgcgaatacc
      961 ggagaatgtt atcacgggag acacacggcg ggtgctaacg tccgtcgtga agagggaaac
     1021 aacccagacc gccagctaag gtcccaaagt catggttaag tgggaaacga tgtgggaagg
     1081 cccagacagc caggatgttg gcttagaagc agccatcatt taaagaaagc gtaatagctc
     1141 actggtcgag tcggcctgcg cggaagatgt aacggggcta aaccatg
//
LOCUS       DAPGOF010000359         1153 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.367, whole genome shotgun sequence.
ACCESSION   DAPGOF010000359 DAPGOF010000000
VERSION     DAPGOF010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.367"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(68..946)
                     /gene="glxR"
                     /locus_tag="RHV13_004771"
     CDS             complement(68..946)
                     /gene="glxR"
                     /locus_tag="RHV13_004771"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HDV1244191.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETAHQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(1046..>1153)
                     /locus_tag="RHV13_004772"
     CDS             complement(1046..>1153)
                     /locus_tag="RHV13_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HDV1244192.1"
                     /translation="LFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
BASE COUNT          287 a          304 c          256 g          306 t
ORIGIN      
        1 ggctgctttc ttttttaata ttgctggccg attcattttc tgatatcggc catttttatt
       61 gcgggtatca ggccagttta tggttagcca ttaattccag cgcctgcact aacgcagagt
      121 gatccaactg gctgccaccg tttgccgcgc aggtattaaa taactcctgg caggtcgcag
      181 tgtttggcag gttcagcgca agtgctttcg cactttgcag tgccaggttg agatctttct
      241 ggtgcagcgc aattttgaaa cctggattaa aggtgcgttt aatcatgcgc tcaccgtgaa
      301 cttccagaat acgtgaggaa gcaaagccgc ccatcagcgc ctggcgcaca cgtaccgggt
      361 ctgcaccggc ttttgaagca aacagcagag cttcagagac cgcttcaata ttgagcgcta
      421 cgataatctg atttgccact ttgcaggttt gaccgtcgcc gttaccgccc acaagggtga
      481 tatttttacc gagcagttca aacagcggtt taacacgttc aaataccgct tcgtcaccgc
      541 caaccataat tgacaacgtc ccttcacgcg cgccgatttc accgccggag actggcgcat
      601 cgagataatc accgcccagt tcattcacct gacgagcgaa acgtttagtt tcaatcgggg
      661 aaatggagct catatcaaca atggttttgc ccttcagcga ggctttggta caaccatttt
      721 caccgaacag aacttcttca acctgtggtg tatccggcac cataataaaa atgatgtccg
      781 atgcttccgt tacctggtgc gcagtttcaa cactgacggc acccagtgac agtaattcat
      841 cagcaaccgg accaatggtt gtgacatgca gttgatgacc ggcacgcgcc agattaatgg
      901 ccatcggtgt acccataatg cctaagccaa taaatcccag tttcataatt aacctctttt
      961 aaatttcgct tttcctgaat tcagacaaca cgatcccctg ccaacttagc ggcctaaaaa
     1021 agcattgcct gaacagcgtt acgttttaac ggtacggatc catccagcgt aaaccggctt
     1081 ccgtggtggt ttggggttta tattcacaac caacccaacc gttgtagtca gaattttcga
     1141 ttaccttaaa gag
//
LOCUS       DAPGOF010000360         1153 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.368, whole genome shotgun sequence.
ACCESSION   DAPGOF010000360 DAPGOF010000000
VERSION     DAPGOF010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.368"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..343
                     /locus_tag="RHV13_004773"
     CDS             <1..343
                     /locus_tag="RHV13_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001626294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1244193.1"
                     /translation="YYSSAQGYQSRLQHTSQDVRELLSKSLDGAASSTLHLKKSGNAF
                     FGSGKYLNEMDFTMNGFSQKIYLMAVNKIENEVKLQCARYFTTGRVDEQNSKIDKVAF
                     TFSATATTADL"
     gene            599..>1153
                     /locus_tag="RHV13_004774"
     CDS             599..>1153
                     /locus_tag="RHV13_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1244194.1"
                     /translation="MVSVTSYTYPTETENDNSQFQSQTQSQGLSQSLRDSIEGNIAAF
                     DAMEQSGLLDNCRTLSKDATFREVFRNILLYILDSFHIYRHDISGKLVSYFHVTPESA
                     NANYKTWIATTGMDSYIKIEQTGSDKFKVTDVYRELKGNSLKKQSRDRDFSGRNVKEF
                     IGLLSEHDALEKYQLSKVTVAANAL"
BASE COUNT          373 a          179 c          242 g          359 t
ORIGIN      
        1 ttattattct tccgcacaag gctatcagag tcgcttacaa cacacatctc aagatgttcg
       61 cgagttactc tcaaagagtt tggatggtgc cgcttcatca acattgcatt taaaaaaatc
      121 aggaaatgca ttttttgggt ctggaaagta tcttaatgaa atggatttca ctatgaatgg
      181 gtttagtcag aaaatatatt tgatggcggt taataaaatt gaaaatgaag ttaagcttca
      241 gtgtgctcgt tattttacta ccggtagggt cgacgaacaa aactctaaaa tcgataaagt
      301 cgcttttaca ttttccgcta ccgcaacaac agctgattta tgattttttc ttcataaagc
      361 gcgtttgtca ttaaggtgag cgcgtattct taaccgtctg tgcgtaaaca gtctttttga
      421 aaaaaaatga gcgatttttg atagtgagta aattgttttg gtgatgcaat tgcattggga
      481 aaatcgttaa aaattaacga cactaaaata tagcggttag cattgacata tttatcatgc
      541 taaccattga tgcttctaaa atgtcaaaat atgcaaagtg tcatattgcg aggttactat
      601 ggttagtgtg acgagttata catatccaac ggaaacagag aatgataata gccagtttca
      661 gagtcaaact cagagtcagg gcctgagcca gagtctaaga gatagtatcg agggtaatat
      721 tgctgcattt gatgcgatgg agcaaagcgg cctgctcgat aattgtagaa cattatctaa
      781 agatgccact tttcgtgaag ttttccgaaa tatattatta tacattttag attcttttca
      841 tatctatcgg catgatatca gtggtaaatt ggtttcttat tttcatgtta cgcctgaatc
      901 agcgaatgcg aattataaga catggatcgc taccacaggc atggatagct atataaaaat
      961 tgagcagacc ggcagtgata agtttaaagt cactgatgtg tatcgtgagt taaaaggcaa
     1021 cagtttgaaa aaacagagta gagacaggga ttttagtggt cgtaatgtga aagagtttat
     1081 aggattatta tcagagcacg atgcattgga aaagtatcaa ctctcaaaag taacagttgc
     1141 tgcaaatgct ctt
//
LOCUS       DAPGOF010000361         1148 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.369, whole genome shotgun sequence.
ACCESSION   DAPGOF010000361 DAPGOF010000000
VERSION     DAPGOF010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.369"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            244..441
                     /locus_tag="RHV13_004775"
                     /pseudo
     CDS             244..441
                     /locus_tag="RHV13_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416065.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            448..1148
                     /locus_tag="RHV13_004776"
                     /pseudo
     CDS             448..1148
                     /locus_tag="RHV13_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755539.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT          328 a          260 c          292 g          268 t
ORIGIN      
        1 gcacgctcga ttggcggtgt ggcatttgat ggctcggcca atatcaatct tccaggtgta
       61 aatacaacag gtaaccagaa caccacgggg aatgctgcta ccgcgacgaa acttgcgacg
      121 gcaagaaaca tcaacggtgt taaatttgat ggttctgtgg acatttcgat accaacaatt
      181 acgtccagag ggcgtgttac tgcgctcacc ggtacaacac aaggtgctgc tactggattg
      241 caaatgtatg aggcgtacaa caacggctat ccttctgctt acgggaatga gcttcatctt
      301 aagggcgcaa cggcagttgg tgaaggtgag ttgttcatag gttggagcgg tacaagtggc
      361 gctcatgcac ctgtacatgt acgttcgcga cgagatactg atacagccag ttggtcagag
      421 tgggcgcagg tctacacatc gaaggattcc atccctggtg taaataccac gggcaaccag
      481 aacacaaccg gaaacgcggc gtctgcaacg aaattacaaa cggcaagaac tattggtggt
      541 gtttcattta atggcaccgc aaacatcgac ctgccgggcg tgaataaaac aggcaatcag
      601 agcactaccg gcaatgcagc tacagccaca aaactgcaaa cagcacgcac cattaatggg
      661 gtttcatttg acggaacggc taatatttct ctgtcaccgg caaatattgg ttgcccggca
      721 tctcctactg gttggcttga aacaggtgat aatggagcat caataacaac agagcaatta
      781 gtgactctgc tacgagataa tggtgctttt aatgcaaagg tatggatagc gcgttgtgcc
      841 tgggcgtatg caattagtgc atccatacca gacagcgaaa caggatgcgg cattattcca
      901 ctggctggcg cagttataga ggtatttaat aatagcagta gcacgagtta ttttaccatt
      961 cgtataacaa cggcaacgac gacaagtgta tctggcgcac tcactaatgc tgaatttatc
     1021 tatgtgtcta atggcacaag ttattcacca ggatggcgca gagcgtataa cactaaaaac
     1081 aaacctactg cggctgatgt cggagcttta ccactatctg gaggagcgtt aactggcgga
     1141 ttaactgc
//
LOCUS       DAPGOF010000362         1127 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.370, whole genome shotgun sequence.
ACCESSION   DAPGOF010000362 DAPGOF010000000
VERSION     DAPGOF010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.370"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..511
                     /locus_tag="RHV13_004777"
     CDS             <1..511
                     /locus_tag="RHV13_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1244195.1"
                     /translation="SARRKKEQDKGATLMEVLLVVGVIVVLAASAYKLYSMVQSNIQS
                     SNEQNNVLTVIANMKSLKFQGRYTDSNYIKTLYAQGLLPSDMIADTTGASAKNPWGGS
                     VTITTSSDKYSFNVVEANVPQKNCMAMVNALRSSSAISKINNTSTSTVSAATVCASDS
                     NTLTFSTDS"
     gene            577..1059
                     /locus_tag="RHV13_004778"
     CDS             577..1059
                     /locus_tag="RHV13_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HDV1244196.1"
                     /translation="MRSVLYILLSCISSLIFSLQPAYSATTTRFDACFAQAGQRYLID
                     PLLLKSIATVESSLIPDRINHNKEKGKILSTDYGLMQINSSHIPNLKKLGVIKDKSEL
                     IDNPCLNIQIGAWILATHFQKCGINWSCLGSYNAGFKESNEQKRIKYARYVYNKYMVR
                     "
BASE COUNT          357 a          258 c          214 g          298 t
ORIGIN      
        1 ttctgcacgc cgtaaaaaag aacaggacaa aggggcaaca ctgatggaag tgttattggt
       61 tgttggtgtt atagttgtac ttgcagcaag tgcatataaa ctctattcaa tggtgcaatc
      121 caacattcaa tcatccaatg aacaaaacaa tgtcctgaca gtgattgcca acatgaaatc
      181 actgaaattc caggggcgtt atactgacag taactatatt aagactctct acgcgcaagg
      241 attactgcca tccgatatga tcgcagatac aactggcgca agcgcgaaaa acccgtgggg
      301 cggctccgtt acaatcacca cctcttcgga taaatactcc ttcaatgttg tagaggccaa
      361 tgtcccacag aaaaactgca tggcaatggt aaacgccctg cgttcttcca gcgcaatctc
      421 caaaattaac aacacttcca catcaaccgt cagtgcagca actgtttgcg catctgacag
      481 caatacgctg accttcagca ctgattcctg atttttcatg tgcatcggat aagcgccttc
      541 gggcgcttat tttttccatg tttaaggagg agtattgtgc gttctgttct ttatatcctg
      601 ctatcatgta tttcatcatt aatcttttca ttgcaacccg catattcagc gacaacgacc
      661 cgatttgatg cctgctttgc acaagccggg caacgttacc tgattgaccc cctgcttctg
      721 aaaagcattg ccactgtaga aagttcactc ataccagacc gaataaatca caacaaagaa
      781 aaaggaaaaa tattaagcac cgattacgga ctaatgcaaa ttaattcatc acacatccct
      841 aacctgaaaa aacttggcgt aataaaagac aaatcagagc ttattgataa cccctgtctc
      901 aatatacaaa ttggcgcgtg gattctggca acacattttc agaaatgtgg cataaactgg
      961 agttgtcttg gcagttataa cgctggtttt aaagaaagta acgaacagaa aagaatcaaa
     1021 tacgcacgct atgtctacaa taaatacatg gtaaggtaag ttatgctaaa cagcatattt
     1081 aatacggcga tactttgctg tgcaaatatc gtctgtcaat gctacgc
//
LOCUS       DAPGOF010000363         1121 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.371, whole genome shotgun sequence.
ACCESSION   DAPGOF010000363 DAPGOF010000000
VERSION     DAPGOF010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.371"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(215..>1121)
                     /locus_tag="RHV13_004779"
     CDS             complement(215..>1121)
                     /locus_tag="RHV13_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1244197.1"
                     /translation="KLVESILVKMGFVGAVVENIYLDSKPFRHIRFVADIPVISFLPH
                     LVKYLKGADHLYSGNDDLHPLFIYFSEAATLKAGPVNLANFRFEVSIASTHLRLVSEQ
                     PDLLFKGFNRDNLEYVYYRSDLASKDLILGLVEHGSPFIKHLHGLIQKRILNEFSLIF
                     SVLEKILESAKPQILACFHSVDYEMNVKIIAEAIPETVSNQIVTSDYIVNSISDADKF
                     INHVRRYLAGRVMKRRIYAELDVCEETSSISLVNLGCFTSPLLVTEYQMFKPHSSGLY
                     RKAINNSLKQFKPEMHVPSEELFYN"
BASE COUNT          382 a          204 c          205 g          330 t
ORIGIN      
        1 tatcgatatc tacatcgtaa tcaaaggtta ctttgtcgaa cgaagtatca gataattgaa
       61 cggttatatc gtcagcggaa acattaaggc ctaaattttg ataaagaaag gcactaacaa
      121 attccacgtg gtcagattct ttaaacattt acactacctc atgactaaaa aggaagtgct
      181 tcccaccggg aagcaggttc ccggatgggt tagattaatt ataaaagagt tcttcagaag
      241 gtacatgcat ttctggtttg aactgtttta aggagttgtt tatagctttt cgatataacc
      301 ctgaagagtg tggcttaaac atttgatatt ccgtaacgag cagaggagac gtaaagcatc
      361 ctaagttcac tagtgaaata cttgatgttt cttcacaaac atctaattca gcgtatatcc
      421 gcctcttcat taccctgcct gctaaatagc gacgaacatg atttataaac ttgtccgcat
      481 ctgatataga attaactata taatcagatg tgactatctg attagataca gtttcaggta
      541 tagcttcggc tattattttt acgttcattt cataatcaac actatggaag caagcaagaa
      601 tttgtggctt cgcgctttct aaaatctttt caagaacaga aaaaatcaga gagaactcgt
      661 ttaaaattcg cttttgtata agtccatgta gatgttttat gaaaggagaa ccgtgttcaa
      721 caaggcctag tataagatcc ttagaggcca aatctgagcg gtaataaacg tattctagat
      781 tatcccggtt aaatcctttg aaaagcaaat caggctgctc tgagaccagt cgtaaatgag
      841 ttgaagctat agaaacttca aaacgaaagt tcgcgagatt tacaggacct gcttttaatg
      901 tagccgcttc ggaaaaataa atgaaaagag gatgcaaatc atcgttacca ctgtaaagat
      961 ggtctgcacc cttaagatat ttcaccaaat gaggtagaaa agatattact ggaatatcag
     1021 ccacaaatcg aatatgccga aatggttttg aatccaaata tatattttcc actacagcgc
     1081 caacaaaacc cattttaaca agaatagatt caactaactt t
//
LOCUS       DAPGOF010000364         1107 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.372, whole genome shotgun sequence.
ACCESSION   DAPGOF010000364 DAPGOF010000000
VERSION     DAPGOF010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.372"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..372)
                     /locus_tag="RHV13_004780"
     CDS             complement(<1..372)
                     /locus_tag="RHV13_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311760.1"
                     /note="may be involved in the export or assemby of
                     proteins involved in the entry of Salmonella into host
                     cells; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscC/YscC/HrcC family type III secretion system
                     outer membrane ring protein"
                     /protein_id="HDV1244198.1"
                     /translation="MKIKLRITIILISVLCIFNGLLTPGAYAAAANGYVANKENLRSF
                     FETVSSYAGKPTIVSKLAMKKQISGNFDLTEPYALIERLSAQMGLIWYDDGKAIYIYD
                     SSEMRNALINLRKVSTNEFNNF"
     gene            complement(369..1091)
                     /locus_tag="RHV13_004781"
     CDS             complement(369..1091)
                     /locus_tag="RHV13_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001453572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV1244199.1"
                     /translation="MNVLLQGQKLTLLDPEMWFLRGNENKDVTLVITIGNNHQKLMVV
                     EDILLLIDRSQIEVTAGKVIYHPLRIDILSKLLAFIDESTGSVEREHHELFADALPFA
                     SEVVLFSKVASEAWFLATYLSSDNINEILFQHLRKTECYKLVRYLLSQSLIQTSLYDL
                     GELYGVSYSHFRRLCSYALGGKVKTELCGWRVARAVLEIIEGNSDMTTIAHKYGYSSS
                     SHFSAEVKSRLGKTPRELCKKL"
BASE COUNT          339 a          238 c          181 g          349 t
ORIGIN      
        1 gaaattatta aactcattcg tcgatacctt tctcagatta atcaatgcat tacgcatctc
       61 tgatgagtca tagatataaa tagctttgcc atcatcatac caaataagcc ccatctgtgc
      121 cgacaggcgt tcaatcaagg catagggttc cgttaaatca aaatttccgc tgatctgttt
      181 cttcatggca agcttactaa ctattgtagg cttacccgca tatgatgaaa ccgtttcaaa
      241 aaaactacgt agattttctt tatttgctac gtatccattc gctgctgcgg catatgcacc
      301 aggagtcaat aatccattaa aaatgcataa gactgaaatt aatataatag taatgcgtaa
      361 ttttattttc ataacttttt acatagttct ctcggtgttt ttcctaaccg gctttttact
      421 tcggctgaaa agtgtgatga ggacgagtaa ccatatttat gcgcaatagt tgtcatatca
      481 ctgtttcctt caatgatttc caacacggct ctggcaactc tccagccaca taattccgtt
      541 ttaactttac cacccaatgc ataactacat aaacggcgaa aatgtgaata cgatacgccg
      601 taaagctcac caagatcata gagtgaagtc tgtatgagcg attgcgataa taaataacga
      661 accagtttat agcattctgt ttttcttaga tgctgaaaca ggatttcatt aatattatcg
      721 ctgctcagat aagtggctaa aaaccaggct tcacttgcaa ctttactgaa tagcacaact
      781 tctgatgcaa agggcaaggc atcagcaaac aattcatgat gttccctttc cacactcccc
      841 gttgattcat ctataaaagc taataattta ctcaggatat caatgcgaag tggatgataa
      901 attactttcc ctgcagttac ctcaatttgg cttcggtcaa tgagcagtaa tatatcttcc
      961 actaccatta atttttgatg gttattacca atcgtaataa caagggttac atccttgttt
     1021 tcatttcccc gtaagaacca catttcaggg tccagtaggg ttaacttctg tccttgaagc
     1081 aaaacattca ttggaagtaa cagccct
//
LOCUS       DAPGOF010000365         1098 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.373, whole genome shotgun sequence.
ACCESSION   DAPGOF010000365 DAPGOF010000000
VERSION     DAPGOF010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.373"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>1098
                     /gene="rhsB"
                     /locus_tag="RHV13_004782"
     CDS             <1..>1098
                     /gene="rhsB"
                     /locus_tag="RHV13_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HDV1244200.1"
                     /translation="WRRERDLTGWMSLSRKPEVTWYGWDGDRLTTIQNDRTRIQTIYQ
                     PGSFTPLIRVETATGELAKTQRRSLADALQQSGGEDGGSVVFPPVLVQMLDRLESEIL
                     ADRVSEESRRWLASCGLTVEQMQNQMDPVYTPVRKIHLYHCDHRGLPLALISAEGATE
                     WCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPI
                     GLKGGWNLYGYQLNPISDIDPLGLSMWEDAKSGACTNGLCGTLSAMIGPDKFDSIDST
                     AYDALNKINSQSICEDKEFAGLICKDNSGRYFSTAPNRGERKGSYPFNSHCPNGTEKV
                     SAYHTHGADSHGEYWDEIFSGKDEKIVKSKDN"
BASE COUNT          300 a          255 c          319 g          224 t
ORIGIN      
        1 tggcgacgtg aacgggacct gacgggctgg atgtcgctgt cacggaaacc ggaagtgacc
       61 tggtacggct gggacggcga ccggctgacc acaatacaga acgacagaac ccgcatccag
      121 acgatttatc agccggggag cttcacgcca ctcatcaggg ttgaaaccgc caccggtgag
      181 ctggcgaaaa cgcagcgccg cagcctggcg gatgcgcttc agcagtccgg cggcgaagac
      241 ggtggcagtg tggtgttccc gccggtgctg gtgcagatgc tcgaccggct ggaaagtgaa
      301 atcctggctg accgggtgag tgaggaaagc cgccgctggc tggcatcgtg cggcctgact
      361 gtggagcaga tgcaaaacca gatggacccg gtgtacacgc cggtgcgaaa aatccacctg
      421 taccactgcg accatcgcgg cctgccgctg gcgcttatca gtgcggaagg ggctacagaa
      481 tggtgcgcag aatacgatga atggggcaac ctgctgaatg aagagaaccc gcatcagctg
      541 cagcagctta tccgcctgcc ggggcagcag tatgatgagg agtccggcct gtattacaac
      601 cgccaccgct attatgaccc gctgcagggg aggtatatca ctcaggatcc gattgggctg
      661 aaggggggat ggaacctgta tggatatcaa ttgaatccga tatcagacat cgaccccctg
      721 ggtttatcta tgtgggagga tgcaaaatcg ggggcatgta ctaatggtct ttgcggcaca
      781 ctatccgcta tgataggtcc agataaattt gattctatag atagcaccgc atatgacgcc
      841 ttaaataaaa taaatagcca atctatttgc gaagataaag agttcgctgg tttaatatgt
      901 aaggataata gtggcagata tttctcaaca gcacctaacc gaggagaaag aaaaggatca
      961 tatccattca atagccattg ccctaatggt actgagaaag tatcagctta tcatactcat
     1021 ggtgcagata gtcatggaga atattgggac gaaatatttt caggtaaaga tgagaaaata
     1081 gttaaaagta aagataac
//
LOCUS       DAPGOF010000366         1084 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.374, whole genome shotgun sequence.
ACCESSION   DAPGOF010000366 DAPGOF010000000
VERSION     DAPGOF010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.374"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(182..>1084)
                     /gene="intI1"
                     /locus_tag="RHV13_004783"
     CDS             complement(182..>1084)
                     /gene="intI1"
                     /locus_tag="RHV13_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HDV1244201.1"
                     /translation="VHWVRAFIRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQ
                     ALAALLFFYGKVLCTDLPWLQEIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQL
                     LYGTGMRISEGLQLRVKDLDFDHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARA
                     WWLKDQAEGRSGVALPDALERKYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQ
                     TFQRAFKRAVEQAGITKPATPHTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYT
                     HVLKVGGAGVRSPLDALPPLTSER"
BASE COUNT          227 a          341 c          336 g          180 t
ORIGIN      
        1 gcactgaacg tcagaagccg actgcactat agcagcggag gggttggatc catcaggcaa
       61 cgacgggctg ctgccggcca tcagcggacg cagggaggac tttccgcaac cggccgttcg
      121 atgcggcacc gatggccttc gcgcaggggt agtgaatccg ccaggattga cttgcgctgc
      181 cctacctctc actagtgagg ggcggcagcg catcaagcgg tgagcgcact ccggcaccgc
      241 caactttcag cacatgcgtg taaatcatcg tcgtagagac gtcggaatgg ccgagcagat
      301 cctgcacggt tcgaatgtcg taaccgctgc ggagcaaggc cgtcgcgaac gagtggcgga
      361 gggtgtgcgg tgtggcgggc ttcgtgatgc ctgcttgttc tacggcacgt ttgaaggcgc
      421 gctgaaaggt ctggtcatac atgtgatggc gacgcacgac accgctccgt ggatcggtcg
      481 aatgcgtgtg ctgcgcaaaa acccagaacc acggccagga atgcccggcg cgcggatact
      541 tccgctcaag ggcgtcggga agcgcaacgc cgctgcggcc ctcggcctgg tccttcagcc
      601 accatgcccg tgcacgcgac agctgctcgc gcaggctggg tgccaagctc tcgggtaaca
      661 tcaaggcccg atccttggag cccttgccct cccgcacgat gatcgtgccg tgatcgaaat
      721 ccagatcctt gacccgcagt tgcaaaccct cactgatccg catgcccgtt ccatacagaa
      781 gctgggcgaa caaacgatgc tcgccttcca gaaaaccgag gatgcgaacc acttcatccg
      841 gggtcagcac caccggcaag cgccgcgacg gccgaggtct tccgatctcc tgaagccagg
      901 gcagatccgt gcacagcacc ttgccgtaga agaacagcaa ggccgccaat gcctgacgat
      961 gcgtggagac cgaaaccttg cgctcgttcg ccagccagga cagaaatgcc tcgacttcgc
     1021 tgctgcccaa ggttgccggg tgacgcacac cgtggaaacg gatgaaggca cgaacccagt
     1081 ggac
//
LOCUS       DAPGOF010000367         1076 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.375, whole genome shotgun sequence.
ACCESSION   DAPGOF010000367 DAPGOF010000000
VERSION     DAPGOF010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.375"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            45..998
                     /gene="ompT"
                     /locus_tag="RHV13_004784"
     CDS             45..998
                     /gene="ompT"
                     /locus_tag="RHV13_004784"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HDV1244202.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETLSFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNSAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVESSDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRV
                     TNKKGNTSLYDHNNNTSDYSKNGAGIENYNFITTAGLKYTF"
BASE COUNT          347 a          227 c          233 g          269 t
ORIGIN      
        1 aaaatacata ttcaatcatt aaaacgattg aatggagaac ttttatgcgg gcgaaacttc
       61 tgggaatagt cctgacaacc cctattgcga tcagctcttt tgcttctacc gagactttat
      121 cgtttactcc tgacaacata aatgcggaca ttagtcttgg aactctgagc ggaaaaacaa
      181 aagagcgtgt ttatctagcc gaagaaggag gccgaaaagt cagtcaactc gactggaaat
      241 tcaatagcgc tgcaattatt aaaggtgcaa ttaattggga tttgatgccc cagatatcta
      301 tcggggctgc tggctggaca actctcggca gccgaggtgg caatatggtc gatcaggact
      361 ggatggattc cagtaacccc ggaacctgga cggatgaaag tagacaccct gatacacaac
      421 tcaattatgc caacgaattt gatctgaata tcaaaggctg gctcctcaac gaacccaatt
      481 accgcctggg actcatggcc ggatatcagg aaagccgtta tagctttaca gccagaggtg
      541 gttcctatat ctacagttct gaggagggat tcagagatga tatcggctcc ttcccgaatg
      601 gagaaagggc aatcggctac aaacaacgtt ttaaaatgcc ctacattggc ttgactggaa
      661 gttatcgtta tgaagatttt gaactcggtg gcacatttaa atacagcggc tgggtggaat
      721 catctgataa cgatgaacac tatgacccgg gaaaaagaat cacttatcgc agtaaggtca
      781 aagaccaaaa ttactattct gttgcagtca atgcaggtta ttacgtcaca cctaacgcaa
      841 aagtttatgt tgaaggcgca tggaatcggg ttacgaataa aaaaggtaat acttcacttt
      901 atgatcacaa taataacact tcagactaca gcaaaaatgg agcaggtata gaaaactata
      961 acttcatcac tactgctggt cttaagtaca cattttaaga acgccaacta aaatttcccc
     1021 gaggtgaaaa tcgccccggg gaataactag ccatttcaat gtaacaatta accctt
//
LOCUS       DAPGOF010000368         1061 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.376, whole genome shotgun sequence.
ACCESSION   DAPGOF010000368 DAPGOF010000000
VERSION     DAPGOF010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.376"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(69..1013)
                     /gene="leuO"
                     /locus_tag="RHV13_004785"
     CDS             complement(69..1013)
                     /gene="leuO"
                     /locus_tag="RHV13_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308107.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HDV1244203.1"
                     /translation="MPEVQTDHPETAEFSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEAASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASRNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKLNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT          300 a          259 c          218 g          284 t
ORIGIN      
        1 aaaattctac attcaataaa aaatgcagaa taaaccagac attcatgtct gacctattct
       61 gcattcagtt agcgtttgca aattgagact aattgctctt ccatccactg atggccttta
      121 tcgcgcccgg cagcttcatg ccaggagaga taacaggttc tgctatttag ttttaacggc
      181 agcggtaata cctgtaattc taaggattca gcgaactctt cagccagcca acgcggcgca
      241 atagcgacca aatgcgtttg cgacaccacg ctaagtacgc tcatcattgc catgccctga
      301 tacgcgatac tggcttgctt atctaccgtg tcataccaag gttgactaaa tgacgcgaaa
      361 cgatcgagcg aaaccgccgc atgttgttcg ttataaacat catgtttcag taacgggccc
      421 ttaattgttg ggtgatttct gctggctacc agcaccattt catctttaaa taatggcacg
      481 ctggtaaatt caggacgatg gaagtcttca taactaatca caaactccgt ttcctgataa
      541 cgcagctgat gttcagtgtt ctgatttaat gaagacttga acataacatg tatatttggc
      601 gcaatctgct caatgtgatt ataaatctgc gaggtcagaa tactgtctaa cgggctgcaa
      661 acacaaagat gaaatacacg ttcactgctc gcggcttcaa aacctgaacc aggcaattca
      721 ttttgtacta gttgcaatgc ctgacgaact gaaccaaaaa gttgaaatgc gcgagcagtc
      781 ggttgaatac cacggccata acgaacaaaa agctcgtcat taaacatcac cttcaggcgt
      841 gcaacagcgt tactgaccgc aggttgcgac attcccagaa catgagcagc acgggtaatg
      901 ttttgctcct gcatcacggc atcgaaaacg gttaataagt tgagatcgac catgcgtagc
      961 tgtggcttgc tgaactccgc cgtctccgga tgatctgttt gtacctctgg catacttaac
     1021 tccactgtca cacttaactc cctttccctt attggaatgc a
//
LOCUS       DAPGOF010000369         1061 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.377, whole genome shotgun sequence.
ACCESSION   DAPGOF010000369 DAPGOF010000000
VERSION     DAPGOF010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.377"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>1061
                     /gene="gltS"
                     /locus_tag="RHV13_004786"
     CDS             <1..>1061
                     /gene="gltS"
                     /locus_tag="RHV13_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HDV1244204.1"
                     /translation="PFLQKNHIPEAVVGGFIVAIVLLIIDKTSGYSFTFDASLQSLLM
                     LTFFSSIGLSSDFSRLIKGGKPLVLLTIAVTILIAIQNTVGMSMAVMMNESPFIGLIA
                     GSITLTGGHGNAGAWGPILADKYGVTGAVELAMACATLGLVLGGLVGGPVARHLLKKV
                     SIPKTTEQERDTIVEAFEQPSVKRKINANNVIETISMLIICIVVGGYISALFKDTFLQ
                     LPTFVWCLFVGIIIRNTLTHVFKHEVFEPTVDVLGSVALSLFLAMALMSLKFGQLASM
                     AGPVLIIIAVQTVVMVLFACFVTFKMMGKDYDAVVISAGHCGFGMGATPTAIANMQTV
                     TKAFGPSHKAFLVVPMVGA"
BASE COUNT          276 a          203 c          231 g          351 t
ORIGIN      
        1 ccgttcctac aaaaaaacca cataccagaa gccgtcgttg gtggctttat tgttgcaatt
       61 gttctgttaa ttattgataa aacatcaggt tattcgttta cttttgatgc ttcattgcaa
      121 agtttattaa tgctcacatt cttttcctct atcgggctaa gttctgactt ttctcgactg
      181 attaaaggag gaaagccgtt agttctatta actattgcag taacgatcct aatcgccatt
      241 caaaatactg tcggcatgag tatggctgtc atgatgaatg aaagtccatt tattggctta
      301 attgcaggtt caattactct aacaggtggt catggtaatg ccggagcatg gggccctatt
      361 ctcgctgata aatatggtgt aacaggcgcc gttgaattag cgatggcttg tgcaacactt
      421 ggattagtgt tgggtggttt agttggcggc cctgttgccc gtcatctttt gaaaaaggtc
      481 tctattccta aaacaaccga gcaagagcgc gacactatcg ttgaagcttt tgagcaacca
      541 agcgtcaaaa gaaaaatcaa tgcaaataac gttattgaaa ccatttcaat gctgattatc
      601 tgtattgttg ttggtggcta tatcagtgca ttgtttaaag atacttttct gcaactgcct
      661 acttttgtct ggtgtttatt tgtcggtatt attatccgta atacactgac tcatgtattt
      721 aaacacgaag tgtttgagcc gaccgtcgat gtattaggta gcgttgcttt atcgcttttc
      781 ttggcaatgg cgttaatgtc attaaaattt ggtcaattgg caagcatggc agggccagta
      841 ttaattatca ttgctgtaca aactgttgtc atggtgctat ttgcctgctt tgtcaccttc
      901 aaaatgatgg gcaaagatta tgatgctgtc gtgatcagcg cgggtcactg tggctttggt
      961 atgggagcaa caccaacagc aattgcgaat atgcaaacag tcacaaaagc atttggacca
     1021 tcacataaag ctttccttgt cgttcctatg gtcggtgcct t
//
LOCUS       DAPGOF010000370         1056 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.378, whole genome shotgun sequence.
ACCESSION   DAPGOF010000370 DAPGOF010000000
VERSION     DAPGOF010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.378"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..236
                     /locus_tag="RHV13_004787"
     CDS             <1..236
                     /locus_tag="RHV13_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lyase"
                     /protein_id="HDV1244205.1"
                     /translation="KNEEVRIEAIIGLSYRKDKRVLSVLCDELKKNTVYDDIIEAAGE
                     LGDKTLLPVLDTMLYKFDDNEIVTSAIDKLKRS"
     gene            261..733
                     /locus_tag="RHV13_004788"
                     /pseudo
     CDS             261..733
                     /locus_tag="RHV13_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT          302 a          216 c          265 g          273 t
ORIGIN      
        1 ataaaaatga ggaagtccgt attgaagcaa taatcggact ttcctacaga aaagataaaa
       61 gggttttatc tgttttatgc gatgagttaa aaaaaaatac tgtttatgat gatatcattg
      121 aagctgcggg tgaattaggt gataaaacgc tacttcctgt tttagatact atgttgtaca
      181 agtttgatga caatgaaatt gtaacttccg ctattgataa gctgaagcgt tcatagcgtg
      241 agctgcctat gcacagtggg gtattcccac cggcacggtg cagacgttcg accagctgga
      301 aagtgaaatc ctggctgacc gggtgagtga ggaaagccgc cgctggctgg catcgtgcgg
      361 cctgacagtg gagcagatgc aaaaccagat ggacccggtg tacacgccgg cgcgaaaaat
      421 ccacctgtac cactgcgacc atcgcggcct gccgctggtg cttatcagca cggaaggggc
      481 aacagaatgg tgtgcagaat gcaatgagtg gggcaacctg ctgaatgaag agaacccgca
      541 tcagctgcag cagcttatcc gcctgccggg gcagcagtat gatgaggagt ccggcctgta
      601 ttacaatcgc caccgctatt atgacccgct gcaggggcga tatatcactc aggatccgat
      661 tgggctgaaa gggggatgga atttttatca gtatccgttg aatccgataa ctgatattga
      721 cccgttggga ttagcgacct gtttatacag cataacttta agtatgcttt catgcgtttc
      781 agatacgcaa aatgatgata actcttatga tgtgcttact attcctgtcg cctctggtaa
      841 taatatgcaa tgtaaaaata atcctggttg tacacactta caaaatagag gtccaatacc
      901 tctaggggta tggagctgga atgttaatgg tcctggagca accaatagaa aacctaatgg
      961 tatacgttta gtaccgtcag caaatacaga aacatataat cgtgatggat ttttaacaca
     1021 tagttgttta aacgctttcg gtccgtcttt aggacc
//
LOCUS       DAPGOF010000371         1046 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.379, whole genome shotgun sequence.
ACCESSION   DAPGOF010000371 DAPGOF010000000
VERSION     DAPGOF010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.379"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            100..924
                     /gene="gadX"
                     /locus_tag="RHV13_004789"
     CDS             100..924
                     /gene="gadX"
                     /locus_tag="RHV13_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HDV1244206.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVFVSRDTLSLFLPMLKEEALNLHAHKKVSSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMKRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
BASE COUNT          304 a          198 c          228 g          316 t
ORIGIN      
        1 tcccgcttcg tttaaattta tttatcaatc aatttgactt aagagggcgg cgtgctacat
       61 taataaacag taatatgttt atgtaatatt aagtcaacta tgcaatcact acatgggaat
      121 tgtctaattg cgtatgcaag acataaatat attctcacca tggttaatgg tgaatatcgc
      181 tattttaatg gcggtgacct ggtttttgcg gatgcaagcc aaattcgagt agataagtgt
      241 gttgaaaatt ttgtattcgt gtcaagggac acgctttcat tatttctccc gatgctcaag
      301 gaggaggcat taaatcttca tgcacataaa aaagtttctt cattactcgt tcatcactgt
      361 agcagagata ttcctgtttt tcaggaagtt gcgcaactat cgcagaataa gaatcttcgc
      421 tatgcagaaa tgctacgtaa aagagcatta atctttgcgt tgttatctgt ttttcttgag
      481 gatgagcact ttataccgct gcttctgaac gttttacaac cgaacatgcg aacacgagtt
      541 tgtacggtta tcaataataa tatcgcccat gagtggacac tagcccgaat cgccagcgag
      601 ctgttgatga gtccaagtct gttaaagaaa aaattgcgcg aagaagagac atcatattca
      661 cagttgctta ctgagtgtag aatgaaacgt gctttacaac ttattgttat acatggtttt
      721 tcaattaagc gagtcgcagt gtcctgtgga tatcacagcg tgtcgtattt catttacgtc
      781 tttcgaaatt attatgggat gacgcccact gagtatcagg agcgatcggc gcagggattg
      841 ccgaaccgtg actcggcggc aagtattgtt gcgcaaggaa atttttacgg cactgaccgt
      901 tctgcggaag gaataagatt atagaatttt actcagacat aaaaaaaacc cggcataggg
      961 gaccgggaag aggatagtct gccgtctcca gactaataaa ccgttataac actccctgtt
     1021 ggcacgggaa actttgtgct ctcagt
//
LOCUS       DAPGOF010000372         1040 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.380, whole genome shotgun sequence.
ACCESSION   DAPGOF010000372 DAPGOF010000000
VERSION     DAPGOF010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.380"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
BASE COUNT          272 a          253 c          244 g          271 t
ORIGIN      
        1 gtggaaaaaa attggcgcag gtgatagcca aattgttacc gcttcagcta ctgcgtggcg
       61 ttggcctgga gcaacggcaa catgtccatc tggcaagaaa gtcattggtg gaggcggtca
      121 atgcagatca aataccggat ttatatggct cacgcgttcc atgccttcag gcaacaatgc
      181 atggactgcc agttgcgaca cgaccgaaga tcaaaatggt tcaattacag tttacgccat
      241 ttgccagtga cgatacttat cactggcaaa ccacgatgta actacatttc cccgacaggt
      301 cagttcggtg agtaccgcca taaaagttgt attcgtcata gaagccgcat ttcacaattc
      361 gattggcatc ataggttgca actgaccccg caaaataatt cggcttccac ccgtgcggat
      421 tttctgcatt actgacactc caggtacact ggctggcaga aaaaccagat ggcaatggga
      481 tctgttgtcc atttgctatt tttcctgtca gaacagtgct acctgatgcc cttctccacg
      541 taccggattg gcacggaggc cagtgccaat ccggtcggtg gtctggtggc aataaagtga
      601 actactccgc ctgtaagtgg tatcaatcat ctgtagcaat gaatcacttc attggcggga
      661 aaagtggagg atctatctac tacaaaccca ttcaatgccc gaccggattt atcatgacag
      721 gtacacgcat gtatggcatt ggtgatggcg ttgatgagga acacgttgat gcttattgtt
      781 gccccttcgg ttaaatccca gcaccaggaa gattcaggca tgtaacagta attgtgccgc
      841 cggactcata atttccccac gcataattat tcacatacca aaccttgttt tggtcggagt
      901 atacctgctg aaacccacca aaaggcgcac cattaaggta tgtccacgaa cagtacgcat
      961 gtacgccaat gctcagattt cgggtgtttt tcccgatatt gtacgtactg gtagtgaagt
     1021 tcacttttgc cgttgtccag
//
LOCUS       DAPGOF010000373         1011 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.382, whole genome shotgun sequence.
ACCESSION   DAPGOF010000373 DAPGOF010000000
VERSION     DAPGOF010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.382"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            13..>1011
                     /locus_tag="RHV13_004790"
     CDS             13..>1011
                     /locus_tag="RHV13_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HDV1244207.1"
                     /translation="MVDNVTVDRVCAQSPSFMPDLDGGKNKTQLIVDDIVAYLKSPSV
                     YSLEKAGPLNHFVNHCSEVELGFYSDGAYSILVSSSKQEPEGMILTVSDADAINILHI
                     VSPARIKFLDDIFTCLHTYADDETFTKEQIKANNKYDVIKYNCKLFFNEKPENKIECR
                     HLSLQYCIDSMNEHSGKVPLKAYYSSPEDIQKHIPFELEQQFHNLQKNPPPGTCVVAS
                     DKFGEALSVFFQRMEKEKLTHMAAIIQSQTHAMAVRLRIKKTPAGETEYVVSFYDPNA
                     TNTAVRYKANNYDSFGTLQSFMNINQETQKWVITDIYFQCVGITPYLPREQAHLLSG"
BASE COUNT          342 a          185 c          183 g          301 t
ORIGIN      
        1 aggggaaaga aaatggttga taacgtaact gttgacagag tatgtgcaca atctccttct
       61 ttcatgcctg atttggatgg aggaaaaaat aaaactcaat tgatcgttga cgatattgtt
      121 gcgtatctta aaagtccttc agtttattca cttgaaaaag cgggtccgtt aaatcatttt
      181 gttaatcatt gttctgaagt tgagttaggt ttctatagcg atggtgcata ttcaattctt
      241 gtctccagct caaagcaaga acctgaaggt atgattttaa ccgtaagcga tgctgacgca
      301 atcaatatat tacatattgt atctcccgca cgtataaaat tcctggatga tattttcact
      361 tgccttcata cgtatgcaga tgatgagacc tttacaaaag agcagataaa agctaataat
      421 aaatatgacg ttataaaata caactgtaag ctttttttta atgaaaaacc agaaaataaa
      481 attgagtgta ggcatctttc tctgcaatat tgtatagatt caatgaatga acattcaggg
      541 aaggttccat taaaggctta ctattcatct ccggaagata tccaaaaaca tattccattc
      601 gagcttgagc agcaatttca caatctacaa aaaaatccac cacccggtac atgcgtcgtt
      661 gccagtgata aatttgggga ggcattatct gtcttctttc aaagaatgga aaaagagaaa
      721 ttaacgcata tggcggcaat cattcaatct caaacacacg ctatggccgt ccgcttgagg
      781 ataaaaaaaa cgcctgctgg agaaacagaa tatgttgtat ccttttatga tcctaatgca
      841 accaatactg cagtacgcta taaagcaaac aactacgatt cttttggcac attgcaatcc
      901 tttatgaaca ttaaccagga aacacaaaaa tgggttataa cagatattta cttccagtgt
      961 gtaggaataa ctccttatct tcctcgagaa caggcccatt tattaagtgg t
//
LOCUS       DAPGOF010000374         1010 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.383, whole genome shotgun sequence.
ACCESSION   DAPGOF010000374 DAPGOF010000000
VERSION     DAPGOF010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.383"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(459..>1010)
                     /gene="hdeD"
                     /locus_tag="RHV13_004791"
     CDS             complement(459..>1010)
                     /gene="hdeD"
                     /locus_tag="RHV13_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HDV1244208.1"
                     /translation="TILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFVSGDILST
                     VVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPELGIFAIAA
                     FIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVSVTLVSTL
                     VGIELIFSAASLFSFASLFVKQQ"
BASE COUNT          339 a          227 c          203 g          241 t
ORIGIN      
        1 tgtttcgact taagctggca attggattgc cagctttctt ttaacgcatt gaaattacct
       61 gagtaaataa ctcattcatg tcttcaggtt tttatatagc atctggagac acggatattt
      121 atgcactgta atcaacgcca gttgcaaaaa aatgaacccg gattacttat ttaaccgccc
      181 atatcatcaa cgcttacgga actctttgtt cactttccca cagcactttt aaaacattaa
      241 acctgactac ggaaaatatc agccatgata atgtttgaaa tggctaattt gccattgaat
      301 gaaaaaaatt agatgaaatt cagtaggttg aaataagcat cagcaggtaa ttatttcaat
      361 gatagtgcgc aactgatcta caacgctgtg tagcggagag agtattaatc ggatcatagt
      421 cacgtcaagt gactatgatc cgggtgacaa cgggataatt attgctgctt aacgaacaaa
      481 ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caattcccac cagtgtggaa
      541 accagcgtta cagacaccat cggcgttgca ccaaggaata tccaggcaat gacgatatcc
      601 agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg ataccaactc
      661 atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc aatggcaaaa
      721 atgcccagct ccggtgcgcg gatgaagaaa tagccgatca ataaataggc gactgcgacg
      781 aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa tccaacaata
      841 agcgcaatgc ccgaacagat taataatgca cctactactg tgcttaaaat atcgccagaa
      901 acgaacggga aactgataca cagcaacccg acgataaaca gcagcacggc aataaactgg
      961 attgctctgc gatgtttttt aagcatctcc agatcaaact tcaaaattgt
//
LOCUS       DAPGOF010000375         1007 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.384, whole genome shotgun sequence.
ACCESSION   DAPGOF010000375 DAPGOF010000000
VERSION     DAPGOF010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.384"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            81..>1007
                     /locus_tag="RHV13_004792"
     CDS             81..>1007
                     /locus_tag="RHV13_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HDV1244209.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLV"
BASE COUNT          293 a          212 c          230 g          272 t
ORIGIN      
        1 aaataatcac attgattaga tgaatattta tcgcgcagtg acatcatttt ttaataatag
       61 ttcaaaaaaa agggctcacg atgaaaaaat taacagtggc aatttctgct gtagctgcat
      121 cagtactgat ggcgatgtct gctcaggcag ctgaaattta taataaagac agtaacaagc
      181 tggatctata tgggaaagtt aatgccaagc actacttctc ctctaatgat gcagatgatg
      241 gtgatactac ttatgcccgt cttggcttca aaggtgaaac ccaaatcaac gatcaactga
      301 ctggtttcgg tcagtgggaa tatgaattca aaggcaaccg cgctgaatct caaggctcct
      361 ccaaagacaa aacccgtctt gcatttgcag gcctgaaatt cggggactac ggctcaatcg
      421 attacggccg taactacggt gtagcatatg acatcggtgc gtggactgac gttctgccag
      481 aattcggtgg cgatacctgg acccaaacag atgtgttcat gactggtcgc accactggtg
      541 ttgcaactta tcgtaacaac gacttctttg gtctggttga tggtctgaac tttgctgctc
      601 agtatcaggg taaaaatgac cgcactgacg taactgaagc taatggtgat ggtttcggtt
      661 tctccactac ttatgagtat gaaggattcg gtgtaggtgc aacctatgct aaatctgacc
      721 gcactaataa tcaggttatc tacggtaaca acagcctgaa tgcatctggt caaaatgctg
      781 aagtatgggc agctggtctg aaatatgatg cgaacaacat ctatctggct accacctatt
      841 ctgaaaccca gaacatgact gtttttggta ataaccatat tgccaacaaa gcacaaaact
      901 tcgaagtagt tgcacaatat cagttcgact tcggtctgcg tccgtccgtt gcttacctgc
      961 aatctaaagg aaaagacttg ggtgcgtggg gtgatcagga cctggtt
//
LOCUS       DAPGOF010000376         1005 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.385, whole genome shotgun sequence.
ACCESSION   DAPGOF010000376 DAPGOF010000000
VERSION     DAPGOF010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.385"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..151)
                     /locus_tag="RHV13_004793"
     CDS             complement(<1..151)
                     /locus_tag="RHV13_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV1244210.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNSWKTVTTQFYIQEFSEYIAPLQD
                     AVDLEI"
     gene            208..876
                     /locus_tag="RHV13_004794"
     CDS             208..876
                     /locus_tag="RHV13_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDV1244211.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     INHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGIVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
BASE COUNT          298 a          219 c          194 g          294 t
ORIGIN      
        1 cgatttccag atctacagca tcctgcagtg gcgcaatata ctcactgaat tcctggatgt
       61 agaactgtgt ggtgacggtc ttccagctat tcggctcctg ctgtattgaa gcataccagg
      121 ctatttcaat atcgctatgc tgcggcagca tttaacccct tgtaattcat tgccataatt
      181 gatttaattc acaaataaaa ctataacatg gtgaaattaa tgaaaaaaaa cacagatgat
      241 ggggctaaaa tttacacacc acttacccta aagctttatg actggtgggt tttgggagta
      301 tcaaatcggc ttgcatgggg atgtcctaca aaggaacacc ttcttccaca ctttctggaa
      361 catttaggta acaaccatct ggatattggt gttggaactg ggttttacct tactcacgta
      421 cctgagagta gtctgatatc tttaatggat ttgaacgaag ctagcctgaa cgcggcatct
      481 acaagggctg gggaatcaaa aattaaacat aaaattaacc atgatgtttt tgaaccttat
      541 cccgcggcgt tacatggtca atttgattcc atttccatgt tttaccttct tcactgcctg
      601 cctggaaata tatctacaaa aagctgtgta atacgcaatg cggcgcaggc cttaactgac
      661 gatggaactc tatacggagc cacaattctt ggcgatggaa ttgtgcacaa tagcttcggt
      721 caaaaactga tgcgcattta caatcagaaa ggcatctttt caaacacaaa agattccgaa
      781 gaaggcttaa cacatatact ctcagagcat ttcgagaatg ttaaaaccaa ggttcaaggt
      841 actgtagtaa tgttttccgc ttcagggaaa aaatagcatc caaccgcagc acgttcttgc
      901 ataaggcgtg tctgcggttt ttcaactatt cagatacatc actcccatca cattcattcc
      961 tccgcatcat aggcatatag gctatatcac cttgatattt ttctt
//
LOCUS       DAPGOF010000377         1003 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.386, whole genome shotgun sequence.
ACCESSION   DAPGOF010000377 DAPGOF010000000
VERSION     DAPGOF010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.386"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..576)
                     /gene="sctT"
                     /locus_tag="RHV13_004795"
     CDS             complement(<1..576)
                     /gene="sctT"
                     /locus_tag="RHV13_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HDV1244212.1"
                     /translation="MGEAILYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALVQHYHYELSTLNEIDIALFAAREIIIGLFIACLLASPFWIFLAIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTNGGLNFILETLWQSYNLWPSG
                     NINFPKLEPLFSYINNIMTHTIVYASPVIAVM"
     gene            complement(578..838)
                     /locus_tag="RHV13_004796"
     CDS             complement(578..838)
                     /locus_tag="RHV13_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HDV1244213.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(848..>1003)
                     /locus_tag="RHV13_004797"
     CDS             complement(848..>1003)
                     /locus_tag="RHV13_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HDV1244214.1"
                     /translation="ISSVLLTLGMMMMSPVTISTPIKLILFVAMDGWTMLSKGLILQY
                     FDLSINP"
BASE COUNT          348 a          206 c          178 g          271 t
ORIGIN      
        1 cataacggca atcacggggc ttgcgtaaac gatagtatga gtcattatgt tattaatata
       61 tgagaataaa ggctctaatt taggaaaatt aatatttcct gaaggccata aattataact
      121 ttgccaaaga gtttccagaa taaagttcaa tccaccgtta gtaagataaa ccgctgcaga
      181 gaataggtta aataatcttg ctaattccga ggtatcgaca cctgtagctg gatccaaagt
      241 actacttaat gttgcaccgc gttggttatc gataaagcta cctatagcaa ggaaaatcca
      301 aaacggggat gctaataagc atgcaataaa cagccctatg ataatttctc tggcagcgaa
      361 caaagcaata tcgatttcat ttaaggtcga taattcatag tggtaatgct gaactaatgc
      421 acatgatacc actattatta ttgggtgacg aactactgtt ggtatattcc cactggcgaa
      481 aaatggtaac aaatagaaag ttggtgccaa tcggcaaaaa ccgagcgctg tagcagccag
      541 cagactatgc aactgataga gtatcgcctc acccatgcta tcctctcgcg aatgctaaat
      601 tcagcatttc aataccaaaa gaatataatt tttcgccata ccatccgctc attaaaaaaa
      661 agcaaatact tacgcataat aatttcacgc caaatggcaa cgtctgttct tgcaactgtg
      721 ttaccgtttg aaaaagccct actaataaac caacaaatgt cgcaactgcg ataggtcctg
      781 cagacataac taaaatcaga tacagcgccc tattccctgc aaaaacaata tcgtccatat
      841 tttttcctca gggattaatg gataggtcaa aatattgtaa aatgagtcct tttgataaca
      901 ttgtccaacc gtccatagcg acaaacagaa ttaattttat cggtgtagat atggttaccg
      961 ggctcatcat catcatacct aacgttaaca aaacgctgga tat
//
LOCUS       DAPGOF010000378          970 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.387, whole genome shotgun sequence.
ACCESSION   DAPGOF010000378 DAPGOF010000000
VERSION     DAPGOF010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..970
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.387"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>970
                     /locus_tag="RHV13_004798"
     CDS             <1..>970
                     /locus_tag="RHV13_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein"
                     /protein_id="HDV1244215.1"
                     /translation="KAYSGEIVILQNEFLKLNSVLGDTKLLPQRKKIENPHPLLQTTV
                     EPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQMEVISALLQEKYH
                     VEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSL
                     GYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVSTPADFRMLAPIVL
                     EQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIVDTQLKNNEVILSGEIP
                     ARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPNSRID"
BASE COUNT          304 a          169 c          205 g          292 t
ORIGIN      
        1 taaggcttat tccggggaaa ttgttatttt gcaaaatgag tttttgaagt taaatagtgt
       61 tcttggagat acaaaactat tgccacagag aaaaaagatt gaaaatccgc accctctact
      121 acaaacaact gttgaaccga gtaaacctga acagagagaa atgttgcttg atgccctttt
      181 ggaaatctca gatagtgatc cgcttctacg atattacgtg gattctacga cacatgaaat
      241 tatactttct ttcttaggga aagtacaaat ggaagtgatt agtgcactgt tgcaagaaaa
      301 gtatcatgtg gagatagaac taaaagagcc tacagtcatt tatatggaga gaccgttaaa
      361 aaatgcagaa tataccattc acatcgaagt gccgccaaat cctttctggg cttccattgg
      421 tttatctgta tcaccgcttc cgttgggaag tggaatgcag tatgagagct cggtttctct
      481 tggatactta aatcaatcat ttcaaaatgc agttatggaa gggatacgct atggctgtga
      541 acaaggattg tatggttgga atgtgacgga ctgtaaaatc tgttttaagt atggcttata
      601 ctatagccct gttagtaccc cagcagattt tcggatgctt gctcctattg tattggaaca
      661 agtcttaaaa aaagctggaa cagaattgtt agagccatat cttagtttta aaatttatgc
      721 gccacaggaa tatctttcac gagcatacaa cgatgctcct aaatattgtg cgaacatcgt
      781 agacactcaa ttgaaaaata atgaggtcat tcttagtgga gaaatccctg ctcggtgtat
      841 tcaagaatat cgtagtgatt taactttctt tacaaatgga cgtagtgttt gtttaacaga
      901 gttaaaaggg taccatgtta ctaccggtga acctgtttgc cagccccgtc gtccaaatag
      961 tcggatagat
//
LOCUS       DAPGOF010000379          958 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.388, whole genome shotgun sequence.
ACCESSION   DAPGOF010000379 DAPGOF010000000
VERSION     DAPGOF010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.388"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..830
                     /gene="yeeO"
                     /locus_tag="RHV13_004799"
     CDS             <1..830
                     /gene="yeeO"
                     /locus_tag="RHV13_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HDV1244216.1"
                     /translation="LGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPL
                     NFSIIWEVMGIGIPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPG
                     SALGSASTIITGRRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGLMASFYTQ
                     DPQVKHVVVILIWLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGY
                     VLGIMLGWGVVGVWMGMFADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
BASE COUNT          195 a          191 c          276 g          296 t
ORIGIN      
        1 gactgggatt tgtcggggca gggctgggtt taaccatttc tcgttatatt ggcgcagttg
       61 caattttgtg ggtgctggcg attggtttta atcctgcgct aaggatttcg ttaaagagct
      121 attttaaacc gctgaatttt agcattatct gggaagtcat ggggattggt attcccgcga
      181 gtgtcgaatc agtgttattt accagtggtc ggttattaac ccaaatgttc gttgccggga
      241 tggggaccag tgttattgcc ggaaatttta tcgcgttttc aattgcggct cttatcaact
      301 tacccggaag tgcgctcggc tctgcttcta cgatcattac aggccgaagg ttgggggtag
      361 ggcagatagc gcaagcagag attcagttgc ggcatgtgtt ctggctgtcc actcttggat
      421 taacggccat cgcctggcta acggctccct ttgccgggct tatggcatcg ttttacaccc
      481 aggatccaca agttaaacat gtcgttgtga ttctgatttg gctaaatgct ttatttatgc
      541 ctatttggtc cgcctcatgg gtgctacccg ctggatttaa aggtgctcgt gatgcccgtt
      601 acgccatgtg ggtttcgatg ttgagcatgt ggggttgtcg ggttgtcgtc ggttatgtgc
      661 tgggaatcat gcttggctgg ggtgtggttg gtgtctggat gggaatgttt gccgactggg
      721 ctgtgcgggc cgtgctgttt tactggcgaa tggttactgg acgttggcta tggaaatacc
      781 ctcgatccga gccgcaaaag tgtgaaaaaa agccagttgt gtcggaataa acgacaaaat
      841 gcagattatt tcagcaaacg atttcaaatt taaaaaacag gctttgacat tgtgggtggg
      901 catcgctaat attcgcctcg ttctcacgat tcctctgtag ttcagtcggt agaacggc
//
LOCUS       DAPGOF010000380          940 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.390, whole genome shotgun sequence.
ACCESSION   DAPGOF010000380 DAPGOF010000000
VERSION     DAPGOF010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.390"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            276..>940
                     /locus_tag="RHV13_004800"
     CDS             276..>940
                     /locus_tag="RHV13_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDV1244217.1"
                     /translation="MAAEHVQTIDLTNFSGRIFFLADPHGHYSALCNLIQSVTKPEEE
                     IVIFSTGNLFDYGPEPIELMKAINSGFFDGRFVRHFSAAGAGEEMLKKLLPLNKDRRT
                     FYPSTYLNERWCVMGGKWHKAVNRYYLEEEVKKLLNTQLGTMMQILLKGDIKIGVCPS
                     DYAPIRTSFTDTYNALQAFNYANVNIFQSQFLFGMDHAVIPMMINDVNLMVLGRNPVN
                     SIR"
BASE COUNT          304 a          180 c          187 g          269 t
ORIGIN      
        1 aatacgtaac tctatgttat ttcagcatcc ttccatttta aatacattac cgttaaatat
       61 cctgtattca tgaagtaaac atgtttataa aggagccaat tataatcaga aagttacttt
      121 gcatcctaag tatgaactgt ttgagagcca ggactggagc ctttaccagt aagtaacaaa
      181 ggtccttagg cttagaatcc aaaatgacgc ctttatgtaa aaaaatcata gctcgaaata
      241 ccgtatcatc gatgtaaaca tacgtagagg aatttatggc agctgagcac gtccaaacta
      301 tagaccttac aaacttctcg ggaaggattt tctttctggc agatcctcat ggacattact
      361 ctgcgctgtg caacttgatt caatcagtta caaagccaga agaagagata gtaattttct
      421 ctactggaaa tttgtttgac tatgggccgg aacccataga gttgatgaag gcgataaaca
      481 gtggcttttt cgatggacgt tttgtgaggc atttctcagc tgccggagcc ggagaagaaa
      541 tgttaaaaaa attgttacca ttaaacaaag accggagaac attttacccc agcacatacc
      601 tcaatgagag atggtgtgta atgggcggaa agtggcataa agctgtaaat cgttactatt
      661 tagaggaaga ggtaaaaaaa cttcttaata cccagcttgg tacgatgatg cagattttac
      721 tcaaaggcga tataaaaatt ggggtttgcc cttctgatta cgcccctatc cgcacatcat
      781 tcactgatac ttataatgcg cttcaagcat tcaactatgc caatgtgaat atttttcaga
      841 gccagtttct attcggtatg gaccatgccg taataccaat gatgataaat gacgtcaacc
      901 ttatggtcct aggtaggaat cctgtaaaca gcattcgaaa
//
LOCUS       DAPGOF010000381          937 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.391, whole genome shotgun sequence.
ACCESSION   DAPGOF010000381 DAPGOF010000000
VERSION     DAPGOF010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..937
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.391"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>937
                     /locus_tag="RHV13_004801"
     CDS             <1..>937
                     /locus_tag="RHV13_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
                     /protein_id="HDV1244218.1"
                     /translation="YDVDENNLKVFNVHFDNNEALVTDGEPDVVCLSKQVWENLLIKL
                     KLENKENTASEVHESANKGEVEQLVECSKRNEQTLFDNIRKSDFHVGSLKPGSMNSVI
                     LEMPPNVCMEPRNSYENKIDEVSSLSEPKEHTIDIQEKTDAFVKKFKGVLFDKNTRSS
                     ELLFNFYECCYVFLPRAQPQDKIESYNSALQAFSIFCSSTLTHNNIGFDFKLFPEVKL
                     SGEHLETVFKYKNGDDVREIAKINITLQKEEGGLYNLRGLDFKGCFFSGQNFSNYDIQ
                     YVNWGTSLFDVDTPCIFNTPANHESYEKSLKPVSEN"
BASE COUNT          323 a          138 c          191 g          285 t
ORIGIN      
        1 atatgatgtt gatgaaaata acttaaaggt attcaatgta cactttgata ataatgaagc
       61 gttagttact gatggtgagc ctgacgtagt atgtttaagc aagcaggtct gggaaaatct
      121 tctcattaaa ctaaagctgg agaacaaaga aaatacggct tctgaagttc atgaatcagc
      181 gaataaaggt gaggttgagc aattagttga atgctctaag agaaatgaac agaccctttt
      241 cgataatata agaaaaagtg attttcatgt tggttcactt aagccaggta gtatgaatag
      301 tgttatttta gaaatgccac caaatgtctg tatggaacca cgtaattcat atgaaaacaa
      361 aatagatgag gtttcatctt tgtcagagcc aaaggaacac accatagata ttcaagaaaa
      421 aacagatgcg tttgtgaaga agttcaaggg ggtattattt gataaaaata caaggtcttc
      481 agagcttctg tttaattttt atgaatgttg ctatgtgttt ttaccaagag cgcagcctca
      541 ggataaaatc gaaagctata attcagcact gcaagctttt tccatctttt gttcatctac
      601 gttgacacat aataatatag gctttgattt caagttattt ccagaagtca agctgtctgg
      661 agaacatctt gaaacggtat tcaaatacaa aaatggcgat gatgtccggg agatagccaa
      721 aattaacatt actctccaaa aagaagaggg tggcttatat aatctacgtg gattggattt
      781 taagggatgc ttcttttctg gacagaactt cagtaactat gatattcaat atgtgaactg
      841 gggaacgtca ttgtttgatg ttgatactcc gtgtattttt aatacgcctg ctaaccatga
      901 gagttatgaa aaatcattaa aacctgtaag cgaaaac
//
LOCUS       DAPGOF010000382          929 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.393, whole genome shotgun sequence.
ACCESSION   DAPGOF010000382 DAPGOF010000000
VERSION     DAPGOF010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.393"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..91
                     /locus_tag="RHV13_004802"
     CDS             <1..91
                     /locus_tag="RHV13_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="effector protein"
                     /protein_id="HDV1244219.1"
                     /translation="VTFAAELFDTQNCNKKQNKKPWEKVMFNV"
     gene            84..>929
                     /locus_tag="RHV13_004803"
     CDS             84..>929
                     /locus_tag="RHV13_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HDV1244220.1"
                     /translation="MFNKVLVGLRSHPELIILGLMVMIIAMLIIPLPTYLIDFLIGLN
                     LTLAILVFLGSFYVDRILSFSSFPSILLITTLFRLALAISTSRLILLEADAGEIITSF
                     GEFVIGDSLVVGFVIFSIVTIVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLR
                     AGIIDADLAKERRSVLERESQLYGSFDGAMKFIKGDAIANIIIIFVNIIGGLSVGVGQ
                     NGMDFSTALTVYTILTVGDGLVSQIPALLIAISAGFIVTRVNGDSDNMGQNIMSQLLS
                     NSFVIV"
BASE COUNT          254 a          150 c          202 g          323 t
ORIGIN      
        1 agtaacattt gcagcagagc tctttgatac acaaaactgt aacaaaaaac aaaataaaaa
       61 accatgggag aaggtgatgt tcaatgttta acaaagtttt ggtagggtta aggagtcatc
      121 cagaattaat aattcttgga ttaatggtga tgattatcgc catgctcata attccattgc
      181 ccacctattt aatcgacttt ttgattgggc ttaacttaac attggcaata cttgtatttt
      241 taggatcatt ctatgttgat aggatcttaa gcttttcttc atttccatca atcctcctta
      301 ttacaacgtt atttcgtttg gcgctggcga tcagtaccag tcgacttata cttcttgagg
      361 cggatgctgg agaaattata acgagttttg gtgagttcgt tattggagat agcctggtag
      421 ttggttttgt tattttctcc attgttacta ttgttcagtt tatcgtgatt actaaaggtt
      481 cggaacgtgt ggctgaagtt gctgcccgct tttcgcttga cggtatgcca ggtaaacaaa
      541 tgagcattga tgccgatcta cgcgctggga tcattgatgc agatttagca aaagaacgtc
      601 gtagcgtatt agaacgagag agtcagctgt atggttcctt tgatggtgcg atgaaattta
      661 tcaaaggtga tgctattgca aacattatta ttatctttgt taatattatc ggtgggctat
      721 cagtaggagt aggccaaaat ggaatggatt tctcaacagc gttgactgtc tacacaattc
      781 ttactgtcgg tgatggtctt gtttctcaaa ttccagcttt attaattgct attagcgcag
      841 gatttatcgt tacgcgcgta aatggcgata gtgataatat ggggcaaaat ataatgtccc
      901 agttattaag taattctttt gtcattgtt
//
LOCUS       DAPGOF010000383          922 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.394, whole genome shotgun sequence.
ACCESSION   DAPGOF010000383 DAPGOF010000000
VERSION     DAPGOF010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.394"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            45..845
                     /locus_tag="RHV13_004804"
     CDS             45..845
                     /locus_tag="RHV13_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1244221.1"
                     /translation="MNSKKLCCICVLFSLLAGCASESSIDEKKKKAQVTQSNMNKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVARAENMNWMQALRFVAAKGHQKAIIV
                     YQDMLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     SRVIFPPAGITKTSNKELSNDHVTEAQLFNLKQKTYVSMVKDLVSRFQ"
BASE COUNT          295 a          176 c          210 g          241 t
ORIGIN      
        1 gctaaatatt ctaatcatta tgacaggcga gggagtgtcc aattatgaat tcaaaaaagc
       61 tttgttgcat atgtgtgtta ttctcgctgc ttgcaggatg tgcctctgaa tcttctattg
      121 atgaaaagaa gaaaaaagct caagtcacac aaagtaatat gaataaaaac actccccagc
      181 aactgacaga caaagattta ttcggtaatg aaaccactct ggccgtatcc gaagaggata
      241 ttcaagcagc gcttgatgga gatgagttcc gcgttcccct taattctcct gtaatacttg
      301 ttcaatccgg caaccgcgca ccggaaacca ttatgcagga ggagatgcga aaatattata
      361 ctgtttccac attctctggt atcccggaca ggcaaaagcc tctgacttgt aacaaaaaca
      421 aagataaaaa tgagaacgag gatgttgccc gtgctgagaa tatgaactgg atgcaggcac
      481 tgcgttttgt ggctgcaaaa ggacatcaga aagcgattat tgtttaccag gatatgttgc
      541 agacaggaaa atatgactct gcgctgaaat caacagtatg gtccgactat aaaaatgaaa
      601 aactcactga cgctatctcc ctgcgctacc tggtacgttt cacgctggtg gatgtggcaa
      661 caggtgagtg ggctacctgg tcgccggtga attatgaatc gagagtaatt tttccgccgg
      721 ctggaataac aaaaacgagc aataaagaac tatcaaatga ccatgttacg gaagcgcaac
      781 tgtttaattt gaaacaaaaa acatatgtat cgatggtgaa agacttggtg agtcgattcc
      841 agtaatcgtt attgttatgc tgccgcctgt agtttagggt ggcagcaaac aggagttgag
      901 tatttttata atgtaaatat ga
//
LOCUS       DAPGOF010000384          917 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.395, whole genome shotgun sequence.
ACCESSION   DAPGOF010000384 DAPGOF010000000
VERSION     DAPGOF010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..917
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.395"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
BASE COUNT          280 a          168 c          183 g          286 t
ORIGIN      
        1 atttgaatgt tcgtaagcta tatcacttac tcaatccatt ttacccagag tcataaaata
       61 gagtgtctgt tgatctcatt ttcatattcc tttcagactg aagtgcattt tcatcacccg
      121 atgtgaaaga actgaagtga tgtttgccat agattgcgag atgccctgtc tcattgacta
      181 tcttggtgca cctttatacc tgttatacca gatcaaaaat cacgcaatcc atacaacaaa
      241 accagatttg caattcgtgt cacaaaatat gtcgatattt ttctaagaag aagatgccat
      301 gtgaagccaa acgaacactt gcggaggcct tcacaaacca agggttgtta tttaattatc
      361 tgttttataa gtaatttatt tagagtataa acaaggaaag atttgcaaga taactcagtg
      421 ggcgtggtga aaataagtgt ttacccgcca tcaggctgag cataattctc atcatgaaat
      481 atgtttcctg gtttgtggct tgtagctggt cactcctgaa gtctctctgg agaggcttgt
      541 tgatgttggt gttttcagga tgatgtttca cttagcttgt ttgccgaata gcccggcgaa
      601 cagctgtgat tgaggaaggt ttaagtcgca gtgaccaaag ccatatttac caacggatgc
      661 agatgaaaaa atcatctcct gctttccccc atatctccag gataaaaagg aatgtaacaa
      721 tctcatgaac taaactgacg aaccagccag gaataatcgc tagggaccta agaattagcc
      781 tgataatagc tactaagaaa ttactgcgtt ccatgaaata gccattttgt ggcaaatgga
      841 gttgtctaat aatgtcatat gtgagacgga tagttgaacg aatattaaat tttgctgaat
      901 tttttatgtt gatttta
//
LOCUS       DAPGOF010000385          911 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.396, whole genome shotgun sequence.
ACCESSION   DAPGOF010000385 DAPGOF010000000
VERSION     DAPGOF010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.396"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            143..322
                     /locus_tag="RHV13_004805"
     CDS             143..322
                     /locus_tag="RHV13_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HDV1244222.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            408..908
                     /locus_tag="RHV13_004806"
     CDS             408..908
                     /locus_tag="RHV13_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HDV1244223.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
BASE COUNT          318 a          183 c          205 g          205 t
ORIGIN      
        1 aagaacgttc ctcataatat attttcccac tttcttctcg ttacgcttaa tttgactaat
       61 tctcattagc gactaatttt aatgagtgtc gacacacaac actcatatta atgaaacaat
      121 gcaacgcaac gggagaaata acatggccga acatcgtggt ggttcaggaa atttcgccga
      181 agaccgtgag aaggcatccg acgcaggccg taaaggcggt cagcatagcg gcggtaattt
      241 taaaaatgat ccgcaacgcg catctgaagc gggtaaaaaa ggcggtcaac aaagcggtgg
      301 taataaatca ggaaaatcct gattcaccgt taattattaa tatcagctga taaagataat
      361 tgcatctgcg ggccgtatgg ctcgcagtga tttcactgga gaaaaatatg aatatgaaga
      421 ccattgaaga tgtatttatt cacctgcttt cagataccta cagtgcagaa aaacaattaa
      481 cccgggcact ggcaaaactc gcaagagcaa catcaaacga aaaattaagt caggcttttc
      541 atgcgcacct cgaggaaact catggacaaa ttgaacgaat tgatcaagtt gtggaatctg
      601 aatcaaacct gaaaattaag cgcatgaaat gtgtggcaat ggaaggtctc attgaagaag
      661 ctaatgaggt catcgaaagc accgagaaaa acgaagtgcg tgatgccgca ctgattgccg
      721 ctgcacagaa agtcgagcac tatgagattg ccagttacgg gactttagcg acgctggctg
      781 aacaattagg ttaccgtaaa gcagcgaagc ttctgaaaga aacgttggaa gaagaaaagg
      841 ccaccgacat caaactgact gatctggccc ttaataacgt aaataagaaa gccgaaaata
      901 aagcctgaaa t
//
LOCUS       DAPGOF010000386          910 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.397, whole genome shotgun sequence.
ACCESSION   DAPGOF010000386 DAPGOF010000000
VERSION     DAPGOF010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.397"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            362..544
                     /locus_tag="RHV13_004807"
     CDS             362..544
                     /locus_tag="RHV13_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001729106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1244224.1"
                     /translation="MSILDKTKDYPAIRRKVVNRLLSGSKLTGKDEEYIFSELKKAEQ
                     SNEREKRLQAIKEYAS"
     gene            complement(632..853)
                     /locus_tag="RHV13_004808"
     CDS             complement(632..853)
                     /locus_tag="RHV13_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDV1244225.1"
                     /translation="MEREFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPELHRRVA
                     EMAMEEDLSLNAFVNKALEKEVSHHRAGA"
BASE COUNT          293 a          213 c          167 g          237 t
ORIGIN      
        1 tttaactgca catctcatta tttacaccgt aaagacaaac acagctttac attgtaaagc
       61 cagatgattg aacactccgt ctccctgata aggaaaaaaa atggctcaag caactccaga
      121 aaagatactg gcaatcatag atgatcatga tcgcgcacaa aaaaaacaac gcaacaaaaa
      181 cctcttaatt tatttatgtc tctgcttagc aattattgga gtggtcgcgt attcctttat
      241 atcaaagttt cgtcttgatg gtgtagccgt ccttacagtg cttttgggcg tgtccgcaat
      301 cgcatttttt ctacattatg tggaatcagg gaaaggccag attcttagta gtgaccgcat
      361 attgtccata ctcgataaaa ccaaagatta ccctgcaatt cgacgtaaag ttgttaatcg
      421 cttgctctcc ggttcaaaac tcacgggtaa agatgaagag tacatattca gtgaattgaa
      481 aaaagctgaa caaagcaacg aacgtgaaaa gagattgcaa gcaattaaag agtatgcatc
      541 gtaatacacc gtaaagcatt gtttgtaaat acaacacact acaccgtaat accaagaaac
      601 atcaacgcct gcgagcaaca aaaaggggcg attaagcccc tgcgcgatga tgactgactt
      661 ctttttccag tgccttattg acaaaggcat tcagcgacaa atcttcttcc atcgccattt
      721 cagcgacccg acgatgcagc tctggatcaa gcctgacgtt aaacacacct ttaaagggcg
      781 tgtcaggctc ctttccgtcc tccacacagg actgtaaata caaatcaacc gatgttttaa
      841 attcccgttc caggtcagcc aatgtttctg cctcataagt caccagatca cgaataaatg
      901 ccagctttcc
//
LOCUS       DAPGOF010000387          904 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.398, whole genome shotgun sequence.
ACCESSION   DAPGOF010000387 DAPGOF010000000
VERSION     DAPGOF010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.398"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..836)
                     /locus_tag="RHV13_004809"
     CDS             complement(<1..836)
                     /locus_tag="RHV13_004809"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDV1244226.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGL"
BASE COUNT          216 a          229 c          234 g          225 t
ORIGIN      
        1 agaccgaatt tatggcctga agcactgatc gatttcacac gcggcaggcg gaagtcccag
       61 acgatatccg gggcgacgaa cggtgccagg aagccaccgc tggcagcgtc gatgtgcatg
      121 tcgatgtcga taccggtgtc ggcctggaat ttatccagcg catcgtgcag cggctgcggg
      181 aactcatagt taccggtgta ggtcacgccg aaagtcggca ccacgccgat ggtgttttcg
      241 tcgcaggctt caatcatgcg tttcgggtcc ataaacaact gaccggggcg catagggatc
      301 tcacgcagct ccacatccca gtagcgggcg aatttatgcc agcagatttg taccggaccg
      361 cacaccaggt ttggtttatc cgttggtttg cctgcagctt ccatacgctt gcgccaacgc
      421 catttcatcg ccatcccgcc gagcatacag gcctcggaag aaccaatggt gttggtgcca
      481 acggcctgac catttttcgg cgcaggcgca tgccacagat cggcaaccat atttacgcaa
      541 cgcaggtcga tggctgcgga ttgcggatat tcttctttgt cgatccagtt tttgttaatg
      601 gataaatcca tcaacttgtg gacgttttcg tcgtcccagg tctggcagaa agtggccagg
      661 ttctgacgag cgttgccatc aagatataat tcgtcattga taatctgaaa tgcgacatcg
      721 tcgcgcattt cgtgcagcgg aaaacgtttt gattctgcga tagtggaaat agacttcgca
      781 ccaaaacgtg aatcgagtag ttccgacctt aaatccgtta cttgcttctt atccatttta
      841 aactccttaa aatgattgga tcgcattaaa aaagtaggat ttatcgataa agtaagcaag
      901 tgca
//
LOCUS       DAPGOF010000388          888 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.399, whole genome shotgun sequence.
ACCESSION   DAPGOF010000388 DAPGOF010000000
VERSION     DAPGOF010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.399"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(88..840)
                     /locus_tag="RHV13_004810"
     CDS             complement(88..840)
                     /locus_tag="RHV13_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000371227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1244227.1"
                     /translation="MDPFSNIPWTAQSEYRALPSVVLLSRRWWLFFFVGGAMDKKICV
                     VSMSVGKPASMTAAWINNELIMAERTSYPERRRDMELQLLRELREKEEKGFIVLVEEE
                     NSFITGRVGQRVRLRDPFMNGRPVLIEAMQIYKELERQKAIKLPRKESGKYILHQSIF
                     DSEHDKKGDEFFNINWSEITTEHVLTLLCCFATEYNNVASAEYIRAMASEVDSRDDSS
                     LIASYINIIKKTQIMGCARVPQGILTGKNNIL"
BASE COUNT          213 a          219 c          195 g          261 t
ORIGIN      
        1 atgtctacac ttcatgataa ataccttcct taacttagga aggttagttg tgggtttacg
       61 cagaaagtaa caaataacct tcgtaggtta tagaatatta ttctttccgg tgagtattcc
      121 ttgaggaact ctagcgcatc ccataatttg agttttcttg atgatgttta tatatgacgc
      181 tatcagcgat gagtcatcac gactatccac ttcagaagcc attgccctga tgtactcggc
      241 gctggcaacg ttgttgtatt ccgtcgcaaa gcaacatagt aacgtcagaa catgctctgt
      301 cgttatttcg ctccagttga tgttgaaaaa ttcatcgcct tttttatcgt gttcggaatc
      361 gaagatgctt tggtggagga tgtatttgcc ggattccttg cgcggtaact tgatcgcttt
      421 ctggcgttcc agctccttgt aaatctgcat tgcttcaatc agtaccggcc tgccgttcat
      481 gaagggatcg cgcaacctta cacgctggcc aactcgacca gtaataaagc tgttttcctc
      541 ttccaccagc acgataaaac ccttttcctc tttttctcgc aattcgcgca gcagctggag
      601 ttccatatcg cggcggcgtt cagggtagct ggtccgctca gccattatca gctcgttatt
      661 gatccatgca gcagtcattg acgccggttt gccgacgctc atcgaaacaa cgcatatttt
      721 cttatccata gcgcccccta caaaaaagaa aagccaccag cggcggctta gcaatacaac
      781 tgaaggtagc gcccggtact cagactgtgc cgtccatgga atatttgaaa agggatccat
      841 ccgtaccggg cgtgtgatga ttctgactga agtcacttgt cagttgtc
//
LOCUS       DAPGOF010000389          865 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.400, whole genome shotgun sequence.
ACCESSION   DAPGOF010000389 DAPGOF010000000
VERSION     DAPGOF010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..865
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.400"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            120..410
                     /locus_tag="RHV13_004811"
     CDS             120..410
                     /locus_tag="RHV13_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001708011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDV1244228.1"
                     /translation="MKVKSTLQYFILAFFLSLTASVAYAGGLDTATNTMTELKTWAFG
                     FGGVCALCYLIYNIIMAFMEKKSWSDVGMALIYCSLAGGALYAGNWALELFK"
     gene            481..801
                     /locus_tag="RHV13_004812"
     CDS             481..801
                     /locus_tag="RHV13_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HDV1244229.1"
                     /translation="MDKVNEHVSESFMTYNGFNRPALVSGIPLMLLLFTAFFAVLTGF
                     PAIFLWGMKGLIIPAICALFLFIVKLACENDPNALRVIRLNLMGLLLKIRHRDLIIGY
                     SSVR"
BASE COUNT          236 a          172 c          183 g          274 t
ORIGIN      
        1 aacgcgaata actatatcgt gccatcaatc cgtgaaaaaa ctgatgaatc agaaataaaa
       61 cccccgaatg atgaagagaa aatatcaccg gactgggatg tttttggaga ttttagttaa
      121 tgaaagtcaa atcaactttg cagtatttta tcctggcgtt tttcctgtca ctaactgcct
      181 ccgttgccta tgcaggtgga ctcgacaccg caacaaatac catgacagaa cttaagacat
      241 gggcatttgg ctttggtggt gtttgtgctt tgtgttacct gatctataac atcatcatgg
      301 cattcatgga gaagaaaagc tggtctgatg ttggaatggc gcttatctat tgttccctcg
      361 ctggcggtgc actttacgcc gggaactggg cgctggaact cttcaaataa gtaacccata
      421 tatacaactg tgccgcatat ttaccttatg tgtgaattgc ggcttttttc atggtgagtt
      481 atggacaaag taaatgaaca tgtcagtgag tcattcatga cttataacgg ttttaaccgt
      541 ccggcgttag tttctggtat ccccttaatg ttgttattgt tcacggcttt tttcgctgtt
      601 ctgacaggct tccctgctat tttcctttgg ggtatgaaag ggctgattat tcctgccata
      661 tgtgcgctgt ttctttttat agtaaaactg gcctgtgaaa atgacccaaa cgccctaaga
      721 gtcatccgtt taaaccttat ggggcttctg ttaaaaatcc gtcacagaga tttaattatt
      781 ggttattcat ctgtcaggta acatatatgc acaaaatcaa attaaaaaac agtgacaaca
      841 aggatgttgc tcgtgaatat cccga
//
LOCUS       DAPGOF010000390          838 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.401, whole genome shotgun sequence.
ACCESSION   DAPGOF010000390 DAPGOF010000000
VERSION     DAPGOF010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.401"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(124..546)
                     /locus_tag="RHV13_004813"
     CDS             complement(124..546)
                     /locus_tag="RHV13_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1244230.1"
                     /translation="MIPSQISFNTLPVNATYASETTVDTQKFSDILYSAGCSLKDMAY
                     LSRCLASIHPTLAKNIYETENLSDQKLLHIDCRSTNDIKINIFFGQQREGLIEISSDT
                     VIFSILSSLIDSAISKFQHQNPVNGSVNPELLYEDYTD"
     gene            complement(627..>838)
                     /locus_tag="RHV13_004814"
     CDS             complement(627..>838)
                     /locus_tag="RHV13_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1244231.1"
                     /translation="LLLDYPPSGNQIHFHIFPDESATLVLYFSNNDLECFVFERDIPS
                     QYRFFKMFTNLALVIDQLTEANKIL"
BASE COUNT          263 a          124 c          186 g          265 t
ORIGIN      
        1 tagctctttt gatttttcgt aaaaaagaga ggtacatgaa ggtgctacca gtatgagtaa
       61 cgttaatcaa cggttttatt acgtctggca ataacgcagg ccatttttcg cgactgggtg
      121 ggtttaatca gtataatcct catacaatag ttcaggatta actgaaccgt taacggggtt
      181 ttgatgttga aatttagaaa tggcggagtc aatcaatgaa gacagtatcg aaaaaataac
      241 tgtgtcagaa ctaatttcaa ttaatccttc tcgttgctga ccaaaaaaga tatttatctt
      301 gatgtcattt gtactacggc aatcaatatg cagtaatttt tgatcgctga gattttctgt
      361 ttcataaatg tttttggcga gagtcgggtg tattgatgcc agacatctgc tcagataagc
      421 catatccttt agggaacacc ccgctgagta gaggatgtca gagaattttt gtgtgtcaac
      481 tgttgtctct gaagcgtatg ttgcattgac tggaagggta ttgaaagaga tctgagatgg
      541 tatcattatt taaatctttc cgtgagtaat aaaaattaat ccccaggcat tgtttagcaa
      601 tatttgccat agggatatat agaaaattaa agtattttat tggcttcggt taactgatct
      661 ataaccagtg ccagattggt aaacattttg aaaaagcgat actggctagg aatatctctt
      721 tcaaagacga aacattcaag gtcattgttt gaaaaataaa ggactagtgt tgctgattcg
      781 tcagggaaaa tatggaaatg tatctggtta ccagaaggag gatagtccag aagaaggg
//
LOCUS       DAPGOF010000391          836 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.402, whole genome shotgun sequence.
ACCESSION   DAPGOF010000391 DAPGOF010000000
VERSION     DAPGOF010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.402"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..>836)
                     /locus_tag="RHV13_004815"
     CDS             complement(<1..>836)
                     /locus_tag="RHV13_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HDV1244232.1"
                     /translation="EIYNKDSNKLDLYGKVNAKHYFSSNDADDGDTTYVRLGFKGETQ
                     INDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAFAGLKFGDYGSIDYGRNYGVAYDIG
                     AWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNNDFFGLVDGLNFAAQYQGKNDRTDV
                     TEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQVIYGNNSLNASGQNAEVWAAGLKY
                     DANNIYLATTYSETQNMTVFGNNHIANKAQNFEVVAQYQFDFGLRPSVAYLQSKGKDL
                     GA"
BASE COUNT          223 a          193 c          184 g          236 t
ORIGIN      
        1 cacgcaccca agtcttttcc tttagattgc aggtaagcaa cggacggacg cagaccgaag
       61 tcgaactgat attgtgcaac tacttcaaag ttttgtgctt tgttggcaat atggttatta
      121 ccaaaaacag tcatgttctg ggtttcagaa taggtggtag ccagatagat gttgttcgca
      181 tcatatttca gaccagctgc ccatacttca gcattttgac cagatgcatt caggctgttg
      241 ttaccgtaga taacctgatc attagtgcgg tcagatttag cataggttgc acctacaccg
      301 aatccttcat actcataagt agtggagaaa ccgaaaccat caccgttagc ttcagttacg
      361 tcagtgcggt catttttacc ctgatactga gcagcaaagt tcagaccatc aaccagacca
      421 aagaagtcgt tgttacgata agttgcaaca cctgtggtgc gaccagtcat gaacacatct
      481 gtttgggtcc aggtatcgcc accgaattct ggcagaacgt cggtccatgc accgatatcg
      541 tatgctacac cgtagttacg gccataatcg atggagccgt agtcaccgaa tttcaggcca
      601 gcgaaggcaa gacgggtttt atctttggaa gaaccttgag attcagcacg gttgcctttg
      661 aattcatatt cccactgacc gaaaccagtc agttgatcgt tgatttgagt ttcgcctttg
      721 aagcccagac gaacataagt tgtgtcgcca tcatctgcat cgttagaaga gaagtagtgc
      781 ttagcattaa cttttccgta cagatccagc ttgttactgt ctttattgta aatctc
//
LOCUS       DAPGOF010000392          832 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.403, whole genome shotgun sequence.
ACCESSION   DAPGOF010000392 DAPGOF010000000
VERSION     DAPGOF010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.403"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            187..>832
                     /locus_tag="RHV13_004816"
     CDS             187..>832
                     /locus_tag="RHV13_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1244233.1"
                     /translation="MSPDNIGSAVNRFSGWPSNDPLKTDEESMTSPVTVQVIKMIAAI
                     LDPHRIVVGIDTLYTTQRETFTSESQQLIESALQTLHVMNLNNLETNRDEEGHYLAFE
                     YNVDTSDRITVSAYNEKKPGCYGQCLLIQDDETDLFNLIKIKIFEARHNYVAQLMSDP
                     EFMFKFSYSAQQNRLEPHLLVPAYFPLKTNEPVTQEDVLLLYRFFKMNDDFNKLT"
BASE COUNT          266 a          161 c          156 g          249 t
ORIGIN      
        1 ttgtttcatt agtaataatc acttaaatgt atcgatgaaa caggtatgac ttaccgggag
       61 tgattgtttc tgagtgaaca gaggccactt tcgcctgata aatgaaaaat gagcgatttt
      121 tgcaagttag tttttctata gccgagtacg ctcagcgcct cgcttgatta caatgaggtt
      181 gctgctatga gtcctgataa tatcggttcg gctgtcaatc gcttctcagg ttggccatct
      241 aatgatcctc taaaaacaga tgaagaatca atgacatcac ccgtgactgt gcaagtaata
      301 aagatgattg ccgccatcct tgacccacat cggatagtcg ttggaattga tacactttat
      361 acaacgcagc gcgaaacatt tacctcagag agtcaacagc tcattgaatc agcattgcaa
      421 acacttcatg tgatgaattt aaataatctg gaaacaaatc gcgatgaaga aggtcactat
      481 ctggcttttg aatataacgt tgacacctct gacagaataa ccgtttctgc ttataatgaa
      541 aaaaagcctg gctgctatgg ccaatgtctt ttaatacagg atgacgaaac agacttattt
      601 aacctgataa aaattaaaat tttcgaagcc aggcataatt atgtcgctca attgatgagc
      661 gatccggaat ttatgttcaa attcagttat agcgctcaac aaaatagatt agaacctcat
      721 cttctggttc ccgcttattt ccctctaaaa acaaacgaac cggttacgca agaggatgta
      781 ctgttacttt atcgtttctt taaaatgaat gatgatttta ataaattaac at
//
LOCUS       DAPGOF010000393          827 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.404, whole genome shotgun sequence.
ACCESSION   DAPGOF010000393 DAPGOF010000000
VERSION     DAPGOF010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.404"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            105..821
                     /locus_tag="RHV13_004817"
     CDS             105..821
                     /locus_tag="RHV13_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDV1244234.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          274 a          145 c          172 g          236 t
ORIGIN      
        1 atttctaata gacgagattt ttcctgttat gatataatat gccgaattaa cacatgttaa
       61 cgatttacca gtaatgtaaa taaattttcg aggagatcat tccagtggga cgtaaatggg
      121 ccaatattgt tgctaaaaaa acggctaaag acggtgcaac gtctaaaatt tatgcaaaat
      181 tcggtgtaga aatctatgct gccgctaaac aaggtgaacc cgatccagaa ttaaacacat
      241 ctttaaaatt cgttattgaa cgtgcaaagc aggcacaagt tccaaagcac gttattgata
      301 aagcaattga taaagccaaa ggcggcggag atgaaacatt cgtgcagggc cgttatgaag
      361 gctttggtcc taatggctca atgattatcg ccgagacatt gacttcaaat gttaaccgta
      421 cgattgctaa cgttcgcaca attttcaata aaaaaggcgg caatatcgga gcggcaggtt
      481 ctgtcagcta tatgtttgac aatactggtg tgattgtatt taaaggatca gaccctgacc
      541 atatttttga aattttactt gaagctgaag ttgatgttcg tgatgtgact gaagaagaag
      601 gtaacattgt tatttatacc gaacctactg accttcataa aggaatcgcg gctctaaaag
      661 cagctggaat cactgagttc tcaacaacag aattagaaat gattgctcaa tctgaagttg
      721 agctttcccc agaagattta gaaatctttg aagggcttgt tgatgccctt gaagatgacg
      781 acgatgtaca aaaagtttat cataacgtcg caaatctcta attatct
//
LOCUS       DAPGOF010000394          827 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.405, whole genome shotgun sequence.
ACCESSION   DAPGOF010000394 DAPGOF010000000
VERSION     DAPGOF010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.405"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            99..299
                     /locus_tag="RHV13_004818"
     CDS             99..299
                     /locus_tag="RHV13_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1244235.1"
                     /translation="MIKPIKLTLIDNYSDLLLTHEVLKRNMEELEHGKRFDQFPEGHD
                     HYEKTVKEAVAIVEARLEVFRK"
     gene            296..>827
                     /locus_tag="RHV13_004819"
     CDS             296..>827
                     /locus_tag="RHV13_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger-like domain-containing protein"
                     /protein_id="HDV1244236.1"
                     /translation="MKSLFFTLDGHMVPASVNVSLNGDGNYYAPIERECNECAGTGHK
                     GADLCEKCHGVQTVGPIIEKLVALEPITFLDNIPELRVNPTLEAWKSRNHVLIEKAKK
                     ANTKFSNSILAQLDNNRFLTERQLDCFFTTFDVMLSETPENEQEILKHSYNIGQVISI
                     ELTFTEVRAAYAKSKGS"
BASE COUNT          286 a          146 c          172 g          223 t
ORIGIN      
        1 gacgaaaata cagcaatcat tgtttctaaa ccaaaattag aagagcttct ggaaattcga
       61 gatctcctta aggaatatta aaaacgagga ttagttgagt gattaaacct ataaagctga
      121 ctcttatcga taattatagt gatttgcttc taactcatga agtccttaaa cgcaacatgg
      181 aagagttaga acacggtaaa cgttttgatc aatttcctga agggcatgac cactatgaaa
      241 aaactgtgaa agaagctgta gcaatcgtag aggcacgttt ggaggtgttt cgaaaatgaa
      301 atctcttttc tttactcttg atggtcatat ggtaccagcc tcagtgaatg tatcacttaa
      361 cggagatgga aattattatg cacctatcga aagagaatgt aacgaatgtg cgggcacagg
      421 ccacaaaggg gctgatttat gtgaaaagtg tcatggggta caaacagttg gacccattat
      481 tgaaaaatta gtggcactag agccaattac ctttctcgat aacattccgg aactgcgtgt
      541 taacccaaca ctcgaagcat ggaaatccag aaaccacgtc cttattgaga aagcaaaaaa
      601 ggctaacact aagttttcta atagtatatt ggcgcaatta gacaataacc gtttcttgac
      661 agaacgccag ctggattgtt tctttacaac ttttgatgtg atgttgtctg aaactccgga
      721 aaatgaacaa gaaattttaa aacatagcta caatatcggc caggtcataa gcattgaact
      781 aacatttact gaagtcaggg ccgcgtacgc aaaaagtaaa gggagtt
//
LOCUS       DAPGOF010000395          817 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.406, whole genome shotgun sequence.
ACCESSION   DAPGOF010000395 DAPGOF010000000
VERSION     DAPGOF010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..817
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.406"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(1..736)
                     /locus_tag="RHV13_004820"
                     /pseudo
     CDS             complement(1..736)
                     /locus_tag="RHV13_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567846.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          244 a          155 c          131 g          287 t
ORIGIN      
        1 aatcccaaat gttcttgact cactagaact ttcaaaggtt tttcagtcaa aaataaatca
       61 caatatccta aagctgcttc ggcatgatga aaatctaaaa gatcattacc attgagctgg
      121 cgtttctgat cccatcttac tcagcatgac aagaggcacc aatatgtaaa gtacggacta
      181 attgtgcaaa ttgtttaagt ttttcatttt ttgatttttt cttattatta atttcatcat
      241 tgttatatcc ggccttgtaa gcctctttga atatctggat taattgctcg ttgaaaaggc
      301 ttaacactcc gtcgaactca atacgatatg cgtgttgata gcttctaagc tcattggtat
      361 gctctttatt agccaagtta agcatttctg ctgttttctg ccaatcaaat tgttcccagc
      421 tttcatagtt gatatggttc atcatttcaa cgagtgaaat atcccacata tgatcaaaaa
      481 aagatttctg aatgatcagt tcttcagctg catcgaaaga tgtatcgtat ggatggtaat
      541 cgccgaaaat ataacttagt ttggtccata ctaattgatc aattggaatg ctgtccttag
      601 ctcccaaaat cgaatacgta gcattacata attcttgttt gactctctca tgactagcta
      661 taagtgtaac tccaaaactc agatgatcaa taattttggc tgtggccatc cttgtttcga
      721 tatcgttctg tttcattagt tctaaaaaaa cagtagcaga tatagggcaa actacctttc
      781 cattatcaac caagtacttt attttttgta ggagatt
//
LOCUS       DAPGOF010000396          816 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.407, whole genome shotgun sequence.
ACCESSION   DAPGOF010000396 DAPGOF010000000
VERSION     DAPGOF010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.407"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>816
                     /locus_tag="RHV13_004821"
     CDS             <1..>816
                     /locus_tag="RHV13_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDV1244237.1"
                     /translation="EHGYILPGTTLLVESLDRLSREKVGEAIERLKLILNHGIDVITL
                     CDNTVYNIDSLNEPYSLIKAILIAQRANEESEIKSSRVKLSWKKKRQDALESGTIMTA
                     SCPRWLSLDDKRTAFVPDPDRVKTIELIFKLRMERRSLNAIAKYLNDHAVKNFSGKES
                     AWGPSVIEKLLANKALIGICVPSYRARGKGISEIAGYYPRVISDDLFYAVQEIRLAPF
                     GISNSSKNPMLINLLRTVMKCEACGNTMIVHAVSGSLHGYYVCPMRRLHRCDRP"
BASE COUNT          266 a          127 c          190 g          233 t
ORIGIN      
        1 agagcatggt tatatattgc caggaactac attgttagtt gaaagtctgg acagactttc
       61 aagagaaaaa gtcggtgaag cgattgaacg tctgaaattg attttgaatc acggtattga
      121 tgttataact ctttgcgaca atacagtcta taatattgac tctttgaatg agccatattc
      181 attaataaaa gccatactta tagcacaaag ggcaaatgaa gaaagcgaga taaagtcaag
      241 tcgggttaaa ttatcatgga agaaaaaacg gcaggatgca ctggaatcag gtacgattat
      301 gacggcgtct tgtccgagat ggctctcctt agatgacaaa agaacggctt ttgttccaga
      361 ccccgacagg gtgaaaacta ttgagctaat ttttaaactc aggatggaaa ggcgctcatt
      421 gaatgcaata gccaagtatt taaatgatca tgctgtaaag aatttctcag gaaaagaaag
      481 tgcatgggga ccttctgtaa ttgaaaaatt attagcgaat aaagctctga taggtatatg
      541 cgtaccttca tatcgtgcaa gagggaaagg gataagtgaa atcgctggct attatcccag
      601 agtcatatca gatgatttgt tttacgctgt acaggaaatt cggttggcac cttttggtat
      661 tagcaatagt agcaagaatc ctatgctaat aaatctactt cgaacagtta tgaagtgtga
      721 ggcttgtggt aataccatga ttgttcatgc ggtatctgga agtttgcatg gctattatgt
      781 ttgtccgatg agaagattac atcgatgtga caggcc
//
LOCUS       DAPGOF010000397          813 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.408, whole genome shotgun sequence.
ACCESSION   DAPGOF010000397 DAPGOF010000000
VERSION     DAPGOF010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.408"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            262..468
                     /locus_tag="RHV13_004822"
     CDS             262..468
                     /locus_tag="RHV13_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1244238.1"
                     /translation="MHKDQYTDAPSQEQVRVKTMLNSTISMGYPDVVIACIEHQVSLE
                     AFRAIEAALVKHDKNTKDYSLVVD"
BASE COUNT          230 a          171 c          170 g          242 t
ORIGIN      
        1 atgctattca acaatacaaa ttatccataa acctcgtttt tacggggttt tgttatattt
       61 aaacattacc gaatagatat aaatctcgat tgtcgatggt attggttgtg tgacaatacc
      121 tagtctttcc ggtatgcctg aagagaatac aaacgacaga ttatgtaagg gaaacgcgct
      181 gaaactttca ctctttcatg ctgagtgatt tgacgttata tttttagccg caagtgaaga
      241 gcaaacgaat ggagcgacaa aatgcataaa gatcaataca ctgatgcccc ctcccaggag
      301 caggttcgtg taaaaactat gcttaatagc accatttcga tgggttaccc ggatgttgta
      361 attgcatgta tagaacatca agtatctctg gaagcattca gggcaattga ggccgcgtta
      421 gtaaagcacg ataagaatac gaaggattat tccctggtgg ttgactgatc accataactg
      481 ctaaccattc tgaccatttc acaagtgaca gagtcaatat cgtattctgt cactgtaatg
      541 ctaatacagt actgcaattc aactacagca atgcctcgta tttaggtgaa tttacaatat
      601 cgtccagttc ggatgccggc tgcatttctg aagataaggc atttcggttc gcgtattttc
      661 ccctcatgct cgtcagtcct gcgcgtagta agaaacagga cactcacact aatttgtgtg
      721 agcatgctgt gatgtccttc tgaattatcc ccatgccatt atgtaacgct ctgtttcaga
      781 tgctcgtcac ggttgtcagg ctgtcgggtc ctc
//
LOCUS       DAPGOF010000398          813 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.409, whole genome shotgun sequence.
ACCESSION   DAPGOF010000398 DAPGOF010000000
VERSION     DAPGOF010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.409"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     repeat_region   109..808
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=231..259
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaactc"
BASE COUNT          163 a          240 c          222 g          188 t
ORIGIN      
        1 ttatctggca tggggaacta ttatgaacat gaggtgttac gtggatatgt tgcttattac
       61 aagtacagct aatataaaaa cttgagaaag agataacggg ttatatggtg gtttatcccc
      121 actggcgcgg ggaactcgag ccctgccaga atggggcctc ttttgtaccc ggtttatccc
      181 cgctggcgcg gggaacactt gatctcgaga gcaatcgccg cgccattgcg cggtttatcc
      241 ccgctggcgc ggggaactca tgctcatcat atatatcaac cgggaactga ccggtttatc
      301 cccgctggcg cggggaactc ccacgatatt tccgccgttc atgatgaaat ggcggtttat
      361 ccccgctggc gcggggaact cgtcatgctc tgccacccgg tcactccagc gtgcggttta
      421 tccccgctgg cgcggggaac tctgctattt tcacttttag aataatcgtc aaaacggttt
      481 atccccgctg gcgcggggaa ctcgctctgt aatgggcctg acgccattaa aagaacggtt
      541 tatccccgct ggcgcgggga actcggcgaa caaccgaccc ctgtcggacc cgacgacggt
      601 ttatccccgc tggcgcgggg aactcatcac acaaccgctc tacagagagc gcgccgacgg
      661 tttatccccg ctggcgcggg gaactccctg gttcagcccc aaccacaggc gtttttcccg
      721 gtttatcccc gctggcgcgg ggaactctac ccagacccat gagttagcgt cccaattttc
      781 ggtttatccc cgctggcgcg gggaactctc taa
//
LOCUS       DAPGOF010000399          810 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.410, whole genome shotgun sequence.
ACCESSION   DAPGOF010000399 DAPGOF010000000
VERSION     DAPGOF010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.410"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            190..792
                     /gene="fimB"
                     /locus_tag="RHV13_004823"
     CDS             190..792
                     /gene="fimB"
                     /locus_tag="RHV13_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HDV1244239.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          233 a          157 c          181 g          239 t
ORIGIN      
        1 tagcgtttgt atggcaacgt tattataagt aactgttgct actccattta agttcactca
       61 gaagaactgg tccacttacg ttagttatta agcaaacgtt cgcttttata aacataatca
      121 ggataaaaat gttggattat tgctaaccca gcacagctag tgcgcgtctg taattataag
      181 ggaaaaacga tgaagaataa ggctgataac aaaaaaagga acttcctgac ccatagtgaa
      241 atcgaatcac tccttaaagc agcaaatacc gggcctcatg cagcacgtaa ttattgtctg
      301 actttgcttt gttttattca tggtttccgg gcgagtgaaa tttgtcgatt gaggatttcg
      361 gatattgatc ttaaggcaaa gtgtatatat atccatcgat taaaaaaagg cttttcaaca
      421 acgcacccgc tattgaacaa agaagttcag gctttaaaaa actggttgag tatccgtact
      481 tcgtacccgc atgctgagag cgaatgggta tttttatcgc gtaagggaaa tccgctttct
      541 cggcaacagt tttaccatat tatctcgact tccggtggta atgccgggtt gtcactggag
      601 attcatccgc acatgttacg ccattcgtgt ggttttgctt tggcgaatat gggaatagat
      661 acgcgactta tccaggatta tcttgggcat cgcaatattc gtcatactgt ctggtatacc
      721 gccagcaatg cagggcgttt ttacggcatc tgggatagag ccagaggacg acagcgtcac
      781 gctgttttat agagatagtt gagataccag
//
LOCUS       DAPGOF010000400          809 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.411, whole genome shotgun sequence.
ACCESSION   DAPGOF010000400 DAPGOF010000000
VERSION     DAPGOF010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..809
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.411"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            50..>809
                     /locus_tag="RHV13_004824"
     CDS             50..>809
                     /locus_tag="RHV13_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDV1244240.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPDIEVNAGYKESIGGAKNFNNKILKHIKSYLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKYPWLYFPLAKSSPGMIVMACNKNYNQH"
BASE COUNT          266 a          156 c          157 g          230 t
ORIGIN      
        1 tagcaaacta tgtgatttcc atttcgattg atttagtgtt tattgacgta tgtactggat
       61 tattaacgat aatatcgagt tctggcctga gcaccgaaaa ttaatatcgg tacataacgc
      121 cgatcttaac gtcgttctga caacgccagc cagtcgttgt ttatcacttc tacttgaagc
      181 ttttcctgat gtggttgcac aacaagattt tttcaccaga gtctgggaag aagaaggtat
      241 gcgtgtgcct actaacacgt tatatcagaa catatccatt atcagacgcg gatttcgcgc
      301 tgttggtgat actacccact cgcttattgc aaccgtgccg agaagaggat tcaagatcca
      361 taatgacatt aacatacaaa atcatgtaat aaactcgtca acagacgcac atacacacaa
      421 tgccccacct gacatagaag ttaatgcggg atacaaagag agcattggtg gcgcaaagaa
      481 tttcaataac aaaatcctca aacatataaa aagttatcta attatgttga gcgcatttgt
      541 cataggcgca tattctgcat attggctatg gaataataat caaccgaagc catttttcaa
      601 ggattacaag actgttgcgg aaattaatgg ctgccatttt aatgtaacag aagatacaat
      661 tgatgggttg aaagagttcg ataaatataa aacacggata ctggattcgg ggattaactg
      721 taaaaaatat ccgtggttat acttccctct tgcaaaatcc tcaccgggga tgattgttat
      781 ggcatgcaat aaaaactata accaacatg
//
LOCUS       DAPGOF010000401          790 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.412, whole genome shotgun sequence.
ACCESSION   DAPGOF010000401 DAPGOF010000000
VERSION     DAPGOF010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.412"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..285
                     /locus_tag="RHV13_004825"
     CDS             <1..285
                     /locus_tag="RHV13_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1244241.1"
                     /translation="PKSNDRETNEYVYDMATFEGISYLGARSPLTQIAEQIKGIREDW
                     KPIAQGAKKVKADVYTSSDRNEERTYLQEQHDLAIKRRDEKREKRLESGE"
     gene            complement(334..>790)
                     /locus_tag="RHV13_004826"
     CDS             complement(334..>790)
                     /locus_tag="RHV13_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1244242.1"
                     /translation="KHGVGYTIINGCITPISNDNEIESVQNAVDNGTDSSRSHFERAL
                     QLMTDREQPDYRNSIKESISAIESLCRKITGNDKGTLGACLKAIEEKGYIHSAMKGAF
                     SQLYGYTSDQGGIRHALTEEDVNPTLAEAKFMLVTCSAFSNYLLSKISD"
BASE COUNT          271 a          148 c          150 g          221 t
ORIGIN      
        1 cctaaatcaa acgatagaga aacaaatgaa tatgtgtatg acatggcgac atttgaagga
       61 ataagttatc taggagcgag aagcccattg acgcaaattg cagaacaaat taaaggtata
      121 agagaggatt ggaaacctat tgcacaagga gctaaaaaag taaaagcaga cgtatatact
      181 tcaagcgata gaaacgaaga acgcacgtat ctgcaagagc aacacgattt ggcaataaaa
      241 aggagagatg agaaaagaga aaaaagatta gagtctggtg aataatttta aagggagtgg
      301 gtaactaacc cactcgtaac tataaacctg taattaatca cttatttttg acaacagata
      361 attactgaac gcactgcaag tgactaacat aaatttagct tcagctaagg taggatttac
      421 atcttcttct gttagcgcat gacgtattcc accctgatca cttgtataac catagagctg
      481 actaaaagcg cctttcattg cagagtgtat atatcctttt tcctctatag ctttaagaca
      541 agcccccaag gttcctttat cattgcccgt gattttcctg cataaagatt caattgcaga
      601 gatagactct ttaatcgagt ttctgtagtc tggctgctct ctatccgtca ttagttgtaa
      661 cgccctttcg aaatggctac gcgatgaatc agtgccatta tcaactgcgt tctgaacact
      721 ttcaatttcg ttatcatttg aaataggagt aatacaacca tttattatgg tataaccaac
      781 gccatgcttt
//
LOCUS       DAPGOF010000402          788 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.413, whole genome shotgun sequence.
ACCESSION   DAPGOF010000402 DAPGOF010000000
VERSION     DAPGOF010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.413"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            108..659
                     /gene="ybcL"
                     /locus_tag="RHV13_004827"
     CDS             108..659
                     /gene="ybcL"
                     /locus_tag="RHV13_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HDV1244243.1"
                     /translation="MKTLIVSTVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGVPEGTKSFAVTVYDPDAPTGSGWWHWTVVNIPATVTYLPVDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTEKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            669..>788
                     /locus_tag="RHV13_004828"
     CDS             669..>788
                     /locus_tag="RHV13_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDV1244244.1"
                     /translation="MLARGKTNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKK"
BASE COUNT          242 a          138 c          185 g          223 t
ORIGIN      
        1 ttgggggtac aattgtgatt gcttttgcat gaacattgcg cctttatgca taatgagata
       61 aaggaatatc aaataaaata acgataggtc ataacaaaga ggtttttatg aaaacactta
      121 tcgtttcaac tgtattggca ttcataacat tttctgcgca ggctgcagca tttcaggtca
      181 ctagtaatga aataaaaaca ggagagcaac ttacaacgtc tcatgtcttt tctggatttg
      241 ggtgtgaagg tggtaataca tcgccctcat taacctggtc tggtgttcct gaaggtacca
      301 aaagctttgc cgtaactgta tatgatccag atgcacctac aggcagtggt tggtggcatt
      361 ggactgttgt taatattcca gcaacagtaa catatttgcc cgttgatgca gggagacgtg
      421 atggaacaaa actgccgact ggtgctgttc aaggccgaaa tgattttggc tatgctgggt
      481 ttggtggcgc atgtcctcct aaaggagata aaccacatca ttaccagttt aaagtatggg
      541 ctctaaaaac tgaaaagatt cctgtagatt ctaactccag cggagcgtta gttggttata
      601 tgcttaatgc taataaaatc gcaaccgctg agataacacc agtttatgag ataaagtagg
      661 gtgagagtat gctggcaaga ggtaagacta acttaaagat cgaagaaata cggatgcata
      721 aacatcatga gattcatagg gttaagcctc ttatgccagc tttgtgtcgt atccgtcagg
      781 gaaagaaa
//
LOCUS       DAPGOF010000403          778 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.414, whole genome shotgun sequence.
ACCESSION   DAPGOF010000403 DAPGOF010000000
VERSION     DAPGOF010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.414"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..432
                     /locus_tag="RHV13_004829"
     CDS             <1..432
                     /locus_tag="RHV13_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV1244245.1"
                     /translation="VVDTTCYFVNGDSTTDIVVEDVVTSAMNQLINNEIYTLKTGSTS
                     HDLEIQCDGNTAPSIEVLASEFDANNFTLNKGTAENIGFAIFLDDGTLTRMRPGLKTP
                     LKDNGSGQYFLNLNAQYARTSGNPVTKGSVESTVTIKISAD"
     gene            482..>778
                     /locus_tag="RHV13_004830"
     CDS             482..>778
                     /locus_tag="RHV13_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDV1244246.1"
                     /translation="MRIITALLMSFFILPVHAGVVIYGTRIIYPEKNREVLVQLMNQS
                     KNASLIQAWIDDGNTTIAPEKIQVPFILTPPVSRVAGGSGQQLKIRKMPNNLPHN"
BASE COUNT          250 a          151 c          153 g          224 t
ORIGIN      
        1 gttgttgata caacgtgtta ctttgttaat ggtgattcga caacagatat cgttgtcgaa
       61 gatgttgtaa ccagtgcaat gaatcaactt atcaataatg aaatctatac tttaaaaacc
      121 ggttcaacct ctcatgactt agaaattcag tgtgacggaa atacagcccc aagtatcgaa
      181 gtactagcat cagagtttga tgctaataac tttacgctga ataaaggaac tgctgagaac
      241 attggctttg ccattttcct cgatgatggg actttgacaa gaatgagacc agggttaaaa
      301 acaccgctga aagataatgg ctctggtcaa tattttctta atttgaatgc acaatatgcc
      361 cgcacaagtg gcaatcctgt tacaaaaggc tccgtagagt caaccgtaac gattaaaatc
      421 agtgcagatt aacgtttttt gccggtagct ctgctaccgg tattatttac ggagtgatta
      481 cgtgagaata ataacagcat tactgatgtc attttttatt ttacctgttc atgccggtgt
      541 ggtcatctat ggtacgcgca ttatttatcc tgaaaaaaac agagaagttc tcgtacagtt
      601 aatgaatcaa agtaaaaatg cctctttaat tcaggcatgg atcgatgatg ggaacaccac
      661 tatcgcgcca gaaaaaattc aggtcccatt tattctcaca ccgccagtaa gtcgtgtagc
      721 aggtggttct ggccaacaac ttaaaataag aaaaatgcca aacaacttgc ctcacaat
//
LOCUS       DAPGOF010000404          776 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.415, whole genome shotgun sequence.
ACCESSION   DAPGOF010000404 DAPGOF010000000
VERSION     DAPGOF010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.415"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            35..667
                     /locus_tag="RHV13_004831"
     CDS             35..667
                     /locus_tag="RHV13_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV1244247.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
BASE COUNT          244 a          131 c          155 g          246 t
ORIGIN      
        1 tatgcaatta tatacggata gggaggttct taacatgggg ctttgtagtc gttataaaag
       61 tcttacatgc aatagttgct caatgcattg ccaaataatg ccagaagagt caccgcgttt
      121 gcagtattgc gcaaactcgt gtttttgtat gtggcccgaa gaaagttcat attttaatcg
      181 tggtgtggta gaaggtatct taacaaaaaa ccacaatgcg agattaagcg gatatatttt
      241 tgtcgatttt tcagtaagtt ttttacgtct attccttgaa aaagactgga ttgactatct
      301 tgcaagtact gacatgggga ttgttttagt cagcgaccgc aatatgcaat cgttagctaa
      361 ttactggcga aaacacaact ctgcaatatc tgctgtcata tataatgatg atggtcttga
      421 tgttgccaat gaaaagatca gacaactatt tattggtcgt tatttatcat ttaccagagg
      481 caacacatta actcagatgg aattcaccat aatggggtat atggtttctg gttataatcc
      541 atatcagatt gctgaagttc tggatatgga tatccgtagc atctacgcgt acaagcaacg
      601 aatcgaaaag agaatgggtg gtaaaataaa cgaattattt attcgttcac attcggtcca
      661 acattgatac tcaaaaatct gccagaatca tactctgttt atacgggcat ttattgaaat
      721 gcccgtattt tataaatagg ccgtcccctc aagagtaaac atcatagtat ttaaaa
//
LOCUS       DAPGOF010000405          768 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.416, whole genome shotgun sequence.
ACCESSION   DAPGOF010000405 DAPGOF010000000
VERSION     DAPGOF010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.416"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..656)
                     /gene="queE"
                     /locus_tag="RHV13_004832"
     CDS             complement(<1..656)
                     /gene="queE"
                     /locus_tag="RHV13_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HDV1244248.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTAWHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     "
BASE COUNT          188 a          206 c          173 g          201 t
ORIGIN      
        1 tatttatggg tttgcatcga caaacgccaa ttacgcgcaa tgcaggtttc aatgcacaaa
       61 cgtgtggcat catccttttg gctaatcggc tgcagtgcaa tgactcgcgg tttatcatcg
      121 gtcagcgttg ccagtagttc atccagtgct tcaatatcac gtacgcgccc cactggatgc
      181 ttgatttcgt tggctcgctc cagtgcctgt gacaacactt catagccgcc gcgcatgttc
      241 agctttggtg atacggtaac ccaggtattc ggtgtgcagc gtacttcatg agtcccactg
      301 gtttcgatct ggcagctaaa accgttcttt tcgagcaggt cagtcagtgg cagcaaatca
      361 tgaatgcaag gctcaccacc cgtaatcacc acatgccacg cggtatatcc ctgacgacca
      421 atgacagcca gcaaatcttc actgctcgcc gccccccact tatcactctc tttggtcttc
      481 gccagaatgc tgaaaaggga gacttcccga tcctcaagct tttcccaggt gtgtttggtg
      541 tcgcaccagg cacagccaac cgggcatccc tgtaaacgaa taaaaatggc ggggacgccg
      601 gtaaagtaac cctcaccttg cagggtctgg aacatctcgt taatcgggta ctgcatagca
      661 ttctctgtga agtggataat tgttaattat tgcagatcct gccgcaacaa tcatgtctta
      721 ttaacattct gttacatgca gatttaaaga aaaatgcgaa acatatcg
//
LOCUS       DAPGOF010000406          751 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.417, whole genome shotgun sequence.
ACCESSION   DAPGOF010000406 DAPGOF010000000
VERSION     DAPGOF010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.417"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            195..>751
                     /locus_tag="RHV13_004833"
     CDS             195..>751
                     /locus_tag="RHV13_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDV1244249.1"
                     /translation="MSRIYAYCRVSTLEKTTKNQRREIESAGFNIKSQQIIEEQISGS
                     AATSERPGFNQLLDRLKRGDALIVTKLDRLGCNPMDISKTVEQLTKAGIKVHCLALGG
                     VDLTSPPGKMMMQVISAVAEFERDLLLERTHSGILRARGAGKRFGRPPVLNDEQIQRV
                     LEQIKSGINISDIAQEFKTSRQTIL"
BASE COUNT          252 a          145 c          161 g          193 t
ORIGIN      
        1 cccggaactc gaaaaacaaa ccgagttaag gccatttttc acaaaatcga ttttgggtct
       61 caccaaaatt acggggttgc atacgcattc gtttattttc gaacgtgtac atacaaatat
      121 gtacaaaaat aatcaaaatt attttctgag atgcattatg atatgtacac caatttcgta
      181 tagaagatct cactatgtct cgaatttatg cttactgtcg ggtatcaact ctggaaaaga
      241 caaccaaaaa tcaacgtcgg gaaatcgaaa gtgcaggttt taacatcaaa tctcagcaaa
      301 taattgaaga acaaattagc ggctcagcag caaccagtga gcgtcctggt tttaaccagt
      361 tgcttgatcg actgaaaaga ggtgatgcat tgattgtcac aaaactggat cgcctcggtt
      421 gtaatccaat ggatatcagt aaaacggtgg aacaactgac caaagcaggt atcaaagtgc
      481 attgcttagc attggggggc gttgacctga ccagtccacc tggaaaaatg atgatgcaag
      541 taatttcagc agtcgctgaa tttgagcgag accttttact tgagcgtact cattcaggga
      601 tattaagagc acggggcgca ggaaagcgat ttggtcgccc acctgtgtta aatgatgaac
      661 aaatacagag ggtactagag caaattaagt caggtataaa tataagtgac attgcccaag
      721 aattcaaaac ctcgcggcaa accattttaa g
//
LOCUS       DAPGOF010000407          751 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.418, whole genome shotgun sequence.
ACCESSION   DAPGOF010000407 DAPGOF010000000
VERSION     DAPGOF010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.418"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(82..624)
                     /gene="fimA"
                     /locus_tag="RHV13_004834"
     CDS             complement(82..624)
                     /gene="fimA"
                     /locus_tag="RHV13_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HDV1244250.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
BASE COUNT          223 a          160 c          151 g          217 t
ORIGIN      
        1 ttgccaccat accgcttcag acaaccggta cattactttg ttataagacg tggtatataa
       61 ttcaaaacaa cgtggttttg attattcgta tctcataata aaagtcgcgt cagcatttgc
      121 ttgccctgct gatgcacttg taccggtgcc tttataacgt gcagaaaaat gaagaacgtt
      181 ggtcccgggg atcaagttct ggttggttga gaagctattc ccatcaggtt tgagaattgc
      241 ggatgtatta tcaagtattt caataccgac acccgttgct gttgttgtat tggtactgga
      301 tgcaatggcc aataaattat cattgattgc atcagcctga ccagaaaatg caacggcagc
      361 attagcggca acaacaggat cgcagtcgtt caactgaatg gtgaatggaa ttaatgcaga
      421 ggtattacca acagcattga aaatatcggt acgatattga ccgagtgtga caacctggtc
      481 tgccgagtca gtattcactg aacaggcagc attcaccagt tcgccttcaa agtgaattgt
      541 accgccatct acaacggcag cataactacc cgtagcggcg aatagtgcgg cagccagcgc
      601 agacgaaata aatcttaatt tcatatatat tccttcaatc tcatttatcg actccacatc
      661 cgtatataac cgattacttt atttaagaca ctgatagtag taaattcctt tttatcctct
      721 aagaatgtct taattgaaaa tatgcactct a
//
LOCUS       DAPGOF010000408          748 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.419, whole genome shotgun sequence.
ACCESSION   DAPGOF010000408 DAPGOF010000000
VERSION     DAPGOF010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.419"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(2..115)
                     /locus_tag="RHV13_004835"
     CDS             complement(2..115)
                     /locus_tag="RHV13_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HDV1244251.1"
                     /translation="MWSSRVGKLQEVEGIIKGMLMTLDIRLGTDIVVIKSN"
     gene            complement(315..>748)
                     /locus_tag="RHV13_004836"
     CDS             complement(315..>748)
                     /locus_tag="RHV13_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV1244252.1"
                     /translation="KTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSKQSLISR
                     CYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNALSAIQT
                     TVKPISEIARESGYKCPSRFTERFHNRFKITPRELRKASRE"
BASE COUNT          220 a          157 c          132 g          239 t
ORIGIN      
        1 atcaattaga tttaataaca acgatatccg tacctaaccg gatatctaat gtcataagca
       61 tccctttaat tatcccctca acctcttgta actttcccac tcttgaactc cacatagtcc
      121 aaacgccgga tgcaaaaaaa tcacacgtgc atttctgaaa ttgtgatgga ggaagatagt
      181 atcaattata tgtgcttata aacgtccccg attagcgcag aagtcaccgg attgttcagg
      241 ctccaatagg ttgagtatgc cgtatttatt ttccaaaata aaaatcgatg ctgttgctcc
      301 ttcataaaat acttttactc tctggacgct tttcttagct ctcttggtgt tatcttaaaa
      361 cgattatgga acctttcagt aaaacgagaa ggacacttgt aaccactttc tctggcaatc
      421 tcgctgatgg gttttactgt cgtttgtata gcagacaacg cattatttaa cctcacgtcg
      481 tccaatatac tttgaaaact tactccctcg cttgccagac ggcgatgcaa tgtggaaaca
      541 gaaatgtaga gatatctcgc taccttgttt gctgtccatt ttgttccagg ttcggatagc
      601 attaggttat aacaacgact tattaatgat tgtttactat ctgataaaag taaacaatta
      661 acatgatgtg ctcctaatga aagtagaacg cccattgcta agtgttcctg aatttgcgtt
      721 gagaagcctc gggaaataga tgttttta
//
LOCUS       DAPGOF010000409          735 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.420, whole genome shotgun sequence.
ACCESSION   DAPGOF010000409 DAPGOF010000000
VERSION     DAPGOF010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.420"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..568)
                     /gene="fimE"
                     /locus_tag="RHV13_004837"
     CDS             complement(<1..568)
                     /gene="fimE"
                     /locus_tag="RHV13_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HDV1244253.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVQRWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLI"
BASE COUNT          230 a          178 c          146 g          181 t
ORIGIN      
        1 ttatgagatt atttctttcc cataatccgg caaaacgagc agcattactg gcggtataac
       61 gtacagtatg gcgaatattt cgatgcccga gataatcctg aattaaacga gtatctgcac
      121 cacgctccgc cagttcataa ccgcaagcat gccttaacat atgaggatga gtctgcgtta
      181 cggttccagc ttcaataccg gcatcgcgaa taatgcgata ggcctgctgg cgagaaagcc
      241 gactcccacg gcgagaaata aatatagcgt cggtccggtc agcgcctttc cagttagcac
      301 gttcctgggt ccagcgttgc acggcttcac gctcatcaaa acgtaacggg tgaacggtag
      361 aaaatccgtt cttcagtcgg cgaatattta ttcgaccttc attaaggtca agatcctgat
      421 aatgcagatc aagcagttca ctaatacgca tcccatgccg atatgccaac agaataagac
      481 aataatctct ggctcccgtt gccccgtaac aaaccgcctg catcatggcc tgaacttctt
      541 taccggtaag ataacgacgt ttactcacaa tagtagtact cctgactgag atatatttga
      601 atgaatacct ataggaaacc tcaatcggtc aaaattagcc caatagataa aaagataacc
      661 acacacgaag tgatgtggct atcagtcaat tacattaaat atcatacaaa aataaaatat
      721 cactgatgtg ataac
//
LOCUS       DAPGOF010000410          708 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.421, whole genome shotgun sequence.
ACCESSION   DAPGOF010000410 DAPGOF010000000
VERSION     DAPGOF010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.421"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            126..362
                     /locus_tag="RHV13_004838"
     CDS             126..362
                     /locus_tag="RHV13_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YcgZ"
                     /protein_id="HDV1244254.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEHATGEEEQKHYNALIGLLFE"
     gene            405..677
                     /locus_tag="RHV13_004839"
     CDS             405..677
                     /locus_tag="RHV13_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HDV1244255.1"
                     /translation="MKTSDNESIKYEITGQAVLQILRMKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKINRIH"
BASE COUNT          240 a          117 c          146 g          205 t
ORIGIN      
        1 tgttgtttta tatgcattag cactaaatgc aaaaaattaa tttatcattc tgtacacata
       61 tttcgtacaa gtttgctatt gttacctcac ttaacagtga ttaacatttt taacagaggc
      121 gtagcatgca tcaaaattca gtgactttag attctgcggg agcaatcact cgttactttg
      181 caaaagctaa cttgcctact cagcaggaaa ctctcgggga aattgtgact gagattttga
      241 aagatggacg taatctgagt cgaaagtcgc tttgtgccaa acttctttgc cgactggaac
      301 acgcgacagg ggaagaggaa cagaaacatt ataatgcact aattgggttg ctttttgaat
      361 aatgcaacag cctgattatt taacaggtta gctatgaagt cgttatgaag acatctgata
      421 atgaaagtat aaaatatgaa attactggcc aggcggtgct ccagatactg cgcatgaaga
      481 taaatttttc attgcagact cttattaagc aattactcgt aatgaaatcg gctgaggaag
      541 atgctttccg acgcgattta attgacagca taattcgtga ttttagcaat agtgattcag
      601 gagggccaaa ccgaagaaca gcgacagctg acaataaaag tatgttcaat ggtaagaaaa
      661 taaacagaat acattaaaat ttcataagta agatgagagg ttaccatg
//
LOCUS       DAPGOF010000411          706 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.422, whole genome shotgun sequence.
ACCESSION   DAPGOF010000411 DAPGOF010000000
VERSION     DAPGOF010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.422"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            117..683
                     /gene="slp"
                     /locus_tag="RHV13_004840"
     CDS             117..683
                     /gene="slp"
                     /locus_tag="RHV13_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HDV1244256.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT          209 a          149 c          160 g          188 t
ORIGIN      
        1 gggatataaa catcagacag gtttacgtta ctatcaggca tatcgcctca gaatcagatg
       61 aaaactataa agaaatatct attatggttt taatatttgt tgataaggat agtaacatga
      121 acatgacaaa aggtgcactc atcctcagcc tttcattttt gcttgccgca tgtagttcaa
      181 ttccgcaaaa tatcaaaggc aataaccaac ctgatattca aaaaagtttt gttgctgttc
      241 ataaccagcc ggggttatat gttggtcaac aagcgcgctt tggtgggaag gttatcaacg
      301 ttatcaatgg caaaacggat acgttgttag aaatcgctgt attaccgttg gatagctatg
      361 cgaagcctga tattgaagcc aactatcagg gccgactgct cgccagacaa agcggcttcc
      421 ttgacccagt gaactatcgt aatcactttg ttaccatcct cggtaccatt cagggtgaac
      481 aaccaggctt tatcaataaa gtcccgtata acttcctgga agtgaatatg cagggcatcc
      541 aggtgtggca tttgagagaa gtggttaata ccacctataa cctgtgggat tacggctatg
      601 gtgcattctg gccggaaccg ggctggggtg cgccttacta caccaatgcg gtgagtcagg
      661 taacacctga gctggtcaaa taacaccacc gaaagatgca gatgct
//
LOCUS       DAPGOF010000412          702 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.423, whole genome shotgun sequence.
ACCESSION   DAPGOF010000412 DAPGOF010000000
VERSION     DAPGOF010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.423"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..680
                     /locus_tag="RHV13_004841"
     CDS             <1..680
                     /locus_tag="RHV13_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000077296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1244257.1"
                     /translation="YLKSYDINEAGQVHVYIGDLAHLPYEEQLYWQAFNEWPKGTISK
                     RAFQTDIVGEWYLSNEPLLALKHKISLMDKNPPTWWKPRGTVLSDAVQYPATDSIKEW
                     GDEILALDQYLVEGFLPKPLSEIAKYEGRIIEPKWGSLRILQEALIAKSATVDEAKML
                     VQPMQKLHALRTEVRGHASTEKKKKAILEARTDHENLRAHFTSLVGQCEASFNDILRI
                     FDFKIDD"
BASE COUNT          226 a          125 c          156 g          195 t
ORIGIN      
        1 ggtatttaaa atcctatgat attaatgagg ccgggcaagt tcatgtatac attggtgatt
       61 tggctcattt accttatgaa gaacagctct attggcaagc cttcaatgaa tggccaaaag
      121 gaacaatttc taagcgtgca tttcagacgg atattgttgg ggaatggtat ctgtccaatg
      181 aaccattgtt ggcactcaaa cacaaaattt cgctaatgga caagaatcct cccacctggt
      241 ggaagccacg gggtacagta ttgagtgacg ctgttcagta tcctgcaaca gattcaatca
      301 aagagtgggg tgatgaaatc cttgcgcttg atcagtattt agttgagggc tttttaccaa
      361 agccactgag tgaaattgct aaatatgagg gccgaatcat tgaacccaaa tggggatcct
      421 tgcgcatact acaagaagct cttattgcta aaagtgcaac agtagatgaa gcgaaaatgt
      481 tagttcagcc tatgcaaaaa ctgcatgctc tacgaaccga agtacgcgga catgcttcta
      541 cagaaaaaaa gaagaaagcg atattagaag cgcgtaccga tcatgaaaat ctacgtgcac
      601 acttcactag ccttgtgggg caatgtgaag catcatttaa tgatatttta aggatttttg
      661 attttaaaat agatgattag ctatttttgt aatagatgat aa
//
LOCUS       DAPGOF010000413          702 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.424, whole genome shotgun sequence.
ACCESSION   DAPGOF010000413 DAPGOF010000000
VERSION     DAPGOF010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.424"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            68..334
                     /locus_tag="RHV13_004842"
     CDS             68..334
                     /locus_tag="RHV13_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001687694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1244258.1"
                     /translation="MLPCTAIIMAVIWIIFFIGDRREKLKHAELDVIKIKARQKIYDR
                     LRYVENEHIVFDPVTGREVPAERTCINKLIEALVDESNDISKRN"
BASE COUNT          233 a          134 c          147 g          188 t
ORIGIN      
        1 tatccttgaa tcagcagcag gaagggccaa agatacattt gctatggctg taaaagtatt
       61 aagctacatg ctcccctgta cggcaattat catggctgtc atatggataa tatttttcat
      121 cggcgataga cgagaaaagt taaaacatgc agagctggac gtgattaaga taaaagctcg
      181 tcaaaaaatc tacgatcgcc tgcgttacgt tgaaaatgag catatcgttt tcgacccggt
      241 tacaggtcgg gaagttccgg cagaacggac gtgtattaat aaactgattg aggcacttgt
      301 tgatgaaagc aacgacatct caaaaagaaa ttgacgaact tctgaatgca ataaaaacag
      361 gacattcaaa attcattcta tcaatacccg tacatactac ccatccaatg gcccataaaa
      421 taatgaagcc ttacttcagg agaacaggac cgcttctgta ttcagatagt atattcagga
      481 tttgaagaaa gcatttattt atatagggca tacaaaaatg agtgctttta ttaagctggc
      541 atctgttctg gcgttattta ttagcccggt tgtggttgca atagcaggag aacaatctca
      601 ctgtacaaaa atcaacgaat accctttcat cgtcactcaa tgcgacgacg gaactgtgac
      661 agtggtaaat gtgataaata accgtgtagc tgtgtgccgt aa
//
LOCUS       DAPGOF010000414          695 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.425, whole genome shotgun sequence.
ACCESSION   DAPGOF010000414 DAPGOF010000000
VERSION     DAPGOF010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.425"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
BASE COUNT          223 a          136 c          137 g          199 t
ORIGIN      
        1 cgagggaaaa caaccgaaga cgttaggagg aaattttaca atactaatag cgaaaaagat
       61 gggcaagtgt tgaatgtaat acacacaaaa taatccactg tacagtcgat tcaaatattt
      121 taccaaggtg tgtttcgtaa ctattttgtg tacaatatga ctctacatat cgacgaaaaa
      181 accacttgat ttgacagaaa cacattcaaa aaaaattctt ttttcatttc aggagcgatt
      241 ttcatctgtt acttgaagta tctgtgagat taagtgactc cccctaacgt cgtactaaat
      301 agtaattact ggccataccg ggagcagtat tcggcgcttc ccacatgcag cagaataacc
      361 gcatcagcga agatgtattg tatttttact atcacaatgt gtacggacgg tttatggaaa
      421 agaatgaaca ttagatacgc gatgcggcgg caggttacac ggttatcact acgatagcca
      481 atactggctg gtgcattacc gccgtgaaca ataggacgcc gtactgctgg aaatttctta
      541 cctttatgac tccccgcgcc gccgtatcgg gaaacgggaa gcaatcacag ggaaagatca
      601 tcagaagttc actttttgta ctaaataatt cgcattttat gtttaaaaat tgagatattc
      661 ctcattacct gaagctgttt tttattgctt ataca
//
LOCUS       DAPGOF010000415          684 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.426, whole genome shotgun sequence.
ACCESSION   DAPGOF010000415 DAPGOF010000000
VERSION     DAPGOF010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.426"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..619
                     /gene="ydeO"
                     /locus_tag="RHV13_004843"
     CDS             <1..619
                     /gene="ydeO"
                     /locus_tag="RHV13_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HDV1244259.1"
                     /translation="KEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEM
                     IAYLNSEERNHHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHP
                     WKLKDICDCLYISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCG
                     YASTSYFIYAFRKHFGNSPKRVSKEYRCQRHTGMNTGNTMNALAI"
BASE COUNT          218 a          136 c          135 g          195 t
ORIGIN      
        1 taaagagatc ggatttactg aaaataccat taaaaaatat ctacaatgca ctaacatcca
       61 gacagtgacg gtgcctgttc ctgcgaagtt tttacgtgct tcaaatgtac cgactggatt
      121 gcttaatgaa atgattgctt atctcaactc ggaagaacgc aatcatcata atttttcaga
      181 acttttgctt ttttcttgcc tgtctatttt tgccgcatgc aaaggtttca ttacactatt
      241 aactaacggt gtgctatccg tttccggaaa agtgagaaat attgtcaaca tgaagctggc
      301 gcacccatgg aagctgaaag atatttgtga ctgcctgtac atcagtgaaa gcctgttgaa
      361 gaaaaaactt aagcaagagc aaacgacatt ctcacagatt cttttagatg ccagaatgca
      421 gcacgcaaaa aatttgatac gcgtagaagg ttcagtcaat aaaattgccg aacaatgtgg
      481 ttatgccagt acatcttatt ttatttatgc gttccgcaaa catttcggca acagtccgaa
      541 gagagtttct aaggagtacc gttgtcaaag gcacacgggt atgaatacgg gcaacacgat
      601 gaatgcttta gctatttgat tatttgctaa cgagtagtca accacgcacc ctacgtaaaa
      661 attaaaaatg gggcagtcat tccc
//
LOCUS       DAPGOF010000416          655 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.428, whole genome shotgun sequence.
ACCESSION   DAPGOF010000416 DAPGOF010000000
VERSION     DAPGOF010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.428"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            187..648
                     /locus_tag="RHV13_004844"
     CDS             187..648
                     /locus_tag="RHV13_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1244260.1"
                     /translation="MSLALHGKSELELAAQKALELLDYVSNITNVDGKILAIMGLITG
                     LSGQAKVSHILFEQAKIHSTDIASLYYYRALVNFHNEKIEEARICIDKSLQLEPRRRK
                     AVVIKECVDMYVPNPLKNNMKLYYKETESESHRVIIDNILKLKQLTRICMR"
BASE COUNT          246 a          110 c          123 g          176 t
ORIGIN      
        1 gctaagcagg agcaaggata taagaatcat tatttatcag atgaaatgtt agccggaaag
       61 aaagaacttt acgagttcac ccctgaaagc atttacaggg ccatgactat atttgatgga
      121 ttacaaaata aaagtgatat acagacgcta aaaacagaat gttattgcct tctagcggaa
      181 tgccatatgt ctttggcact tcatggaaaa agtgaacttg aacttgctgc tcaaaaagca
      241 ttagagcttt tagattatgt atcaaacata accaatgtcg atggaaaaat tttagctatt
      301 atgggactga taactggcct gtctggacaa gcaaaagtat ctcatatctt atttgaacag
      361 gctaagatac actcaactga tatagcctct ctctactact atagggcact tgtcaacttt
      421 cataatgaaa aaattgaaga ggcaaggatt tgtatagaca aatcactaca actcgaaccc
      481 agaagacgaa aagcagttgt gataaaagaa tgtgtagata tgtatgtgcc taacccgctc
      541 aaaaacaaca tgaaactcta ctataaagaa actgagagtg aaagccatcg agttataatt
      601 gacaacattt tgaaactaaa gcagctgacg agaatttgta tgcgataaat tttta
//
LOCUS       DAPGOF010000417          647 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.430, whole genome shotgun sequence.
ACCESSION   DAPGOF010000417 DAPGOF010000000
VERSION     DAPGOF010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.430"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>647
                     /locus_tag="RHV13_004845"
     CDS             <1..>647
                     /locus_tag="RHV13_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDV1244261.1"
                     /translation="NIIFYKVGENLIMRGTISIVNEVVKTYSLPIEKDDNGYYDFSGL
                     YLAHSNISGKDPNKDPAIDFGIDLGNCNCSNVNFEHTYFDSVKFTNTNCTNANFNSCR
                     FIKCDLTNMNCTGAILDNAVIYGKEKEPEMQYPEADQIIQRITYQKSHGNETKGMILT
                     NCSCVKTTFNWADLSESDCQNVDFSEANLSNTILPDIVRMKGTKLYRTDLFNPIL"
BASE COUNT          231 a          113 c          117 g          186 t
ORIGIN      
        1 ataacattat tttttataaa gtcggagaaa atctgatcat gcgtggaact atctctattg
       61 ttaacgaggt agtaaagact tattcactcc caatagaaaa ggatgacaat ggatattacg
      121 atttcagcgg tctatatcta gcacacagca atatttccgg taaagatccc aataaagatc
      181 ccgctataga tttcggtata gatctgggaa actgtaactg ctcaaatgtc aacttcgaac
      241 atacttattt tgatagcgtc aagtttacaa atacgaattg tacaaatgcg aattttaaca
      301 gttgtagatt tatcaaatgc gatctaacaa atatgaattg cactggcgca attcttgata
      361 atgcggtgat atatggtaaa gaaaaagaac ctgagatgca atatccagaa gcagatcaaa
      421 taatacaaag gataacttat cagaaatcac atgggaatga aacgaaaggg atgattctaa
      481 ctaactgctc ctgtgttaaa accactttca attgggcaga tttatcagaa agtgattgcc
      541 aaaatgtgga ctttagcgag gctaatcttt caaacactat tcttcctgac atcgtcagga
      601 tgaaaggcac gaaactttac cgtaccgacc tttttaatcc gatatta
//
LOCUS       DAPGOF010000418          623 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.431, whole genome shotgun sequence.
ACCESSION   DAPGOF010000418 DAPGOF010000000
VERSION     DAPGOF010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.431"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(376..606)
                     /locus_tag="RHV13_004846"
     CDS             complement(376..606)
                     /locus_tag="RHV13_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1244262.1"
                     /translation="MNTICSLIVVVALSLLSFSSVAKTITAVGSTIDSTEKEISLQAE
                     KQGKSYKILGAFFKNRVYMIAKLTPVSKNDAS"
BASE COUNT          190 a          121 c          111 g          201 t
ORIGIN      
        1 aatatgcatc cttttcctgc ttttcaagtt gttcagcggc gtaagaaaac gtattaaata
       61 tgaattcgac gatttaacca tttctgtctg attggtaatc atctgtgatt aatgctggtc
      121 tattgattac gccagcatta accgcttagg gatgtattct tcctgatcat atacagccct
      181 cgaaaaagag agaaaataaa actgtgtcca atattactct accactccag accgaagcat
      241 cggtagaatt agcagaacga ctggtacaga aggtgggtat agcgtcattt gtcgggcatt
      301 ccattgccat cataatgata attttgaatc aatagacgtg cttcttgatc atgtcttaaa
      361 attataccat gagccttatg aagcatcatt tttactgact ggtgttaact ttgctatcat
      421 ataaactctg ttcttaaaaa acgcgcccag aattttatat gatttccctt gtttttctgc
      481 ctgtaaagaa atttcttttt cagtgctgtc aatagttgaa ccgacggcag taatcgtttt
      541 ggcgacgcta ctgaatgata ataaagacag tgcaaccaca acaattaaag aacagatagt
      601 attcatgcta attctcctga caa
//
LOCUS       DAPGOF010000419          623 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.432, whole genome shotgun sequence.
ACCESSION   DAPGOF010000419 DAPGOF010000000
VERSION     DAPGOF010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.432"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..590
                     /locus_tag="RHV13_004847"
     CDS             <1..590
                     /locus_tag="RHV13_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001425343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TrbJ"
                     /protein_id="HDV1244263.1"
                     /translation="PSIFASGIPVVDVTAIAKTVEEGLNRAAEAARQLEQLKQQYEQT
                     IRYAEEQKKRLEGFTDFSNGFDSASSYMKDSLSTITNSAKSDLSSLRSQYDLSSNVAE
                     TQQKYDAILAKIKFYENFNNEMHERAERLTTLQKEFASADTPQKKADLSNQLNTEKLT
                     MELQLKQYDIAERQLENERKAQYETYVRQKRRDLS"
BASE COUNT          237 a          119 c          128 g          139 t
ORIGIN      
        1 caccatcaat ctttgcgtct ggtattccag tagtagacgt tacggcgata gctaaaacag
       61 ttgaagaagg gctaaatcgt gccgctgaag cggcaagaca gcttgagcaa ctcaagcaac
      121 aatatgaaca gaccataaga tacgctgaag aacagaagaa aagactcgaa ggcttcactg
      181 atttcagcaa tggctttgat agcgcatcca gctatatgaa ggattctcta tcaacaataa
      241 caaatagtgc aaaatcagat cttagctctt tgcgatcgca atatgactta tcaagcaacg
      301 ttgcagagac gcaacaaaaa tatgatgcaa tcctggctaa gatcaaattc tatgaaaatt
      361 tcaacaatga aatgcatgaa agggctgaga gattaaccac gttacagaag gagttcgcgt
      421 cagcagatac tccgcaaaaa aaagctgact tatctaacca attgaataca gaaaaattga
      481 caatggagct acaattgaag caatatgata ttgctgaacg gcaactagag aatgagcgga
      541 aagctcaata tgaaacatat gttcgccaaa agagaagaga tttaagctga tatgaataag
      601 tcacgaaatg caattatcgc ctc
//
LOCUS       DAPGOF010000420          621 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.433, whole genome shotgun sequence.
ACCESSION   DAPGOF010000420 DAPGOF010000000
VERSION     DAPGOF010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.433"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            20..187
                     /locus_tag="RHV13_004848"
     CDS             20..187
                     /locus_tag="RHV13_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HDV1244264.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTA
                     ITEPDAKTQSR"
     gene            complement(247..309)
                     /locus_tag="RHV13_004849"
                     /pseudo
     CDS             complement(247..309)
                     /locus_tag="RHV13_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
BASE COUNT          175 a          129 c          131 g          186 t
ORIGIN      
        1 tataaactac aagtggatat tgtgtcctgt atgtggaaat aaaacacgat taaagataag
       61 ggaagatact gaattaaaaa aattccccct ctattgtccg aaatgcagac aagaaaattt
      121 aattgaaata aagcagttca aagtaactgc gattacagag ccagacgcaa agacgcagag
      181 ccgataaaat gagattaata caatctcatt ttatcggctc tttccgttat gtatggattc
      241 ttttaattag tcttcgatgt ttcttgcttc gttgataccg ctggctaaag attccattaa
      301 ggatagttca agtatagcct gaattagccc tcgattttac atcttggaac catggccccc
      361 ccctttttta ggaaaaggat gaccttcggt ctctctattt aaaggaaatc ttcacgcctt
      421 tttgtcggat tcggctgaaa tttacgggac ggtcttattt aagctctcca cgatggcatc
      481 ctgccggctt aggtcttgtt cctccgtgct cacgcgcagg tagatgcgtg cgactttcat
      541 gcgggcctcc tggtcatttt gggtataggg aaaatgacca ttgtttcacg cctagccaaa
      601 aagggaaggt tcccggttca a
//
LOCUS       DAPGOF010000421          618 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.434, whole genome shotgun sequence.
ACCESSION   DAPGOF010000421 DAPGOF010000000
VERSION     DAPGOF010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.434"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..361)
                     /locus_tag="RHV13_004850"
     CDS             complement(<1..361)
                     /locus_tag="RHV13_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2004 domain-containing protein"
                     /protein_id="HDV1244265.1"
                     /translation="MSECHKTGLQYIIVHQSEILASILSELMKKYPDLQGQYDYDEEE
                     KELYMPDVGTQEDFSKLLSPLAIHILSVHNNGQPYIGFEFSCSWDIEHDFGVMMLYDC
                     VVKLGGADTSFLTWIAEE"
     gene            complement(401..>618)
                     /locus_tag="RHV13_004851"
     CDS             complement(401..>618)
                     /locus_tag="RHV13_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1244266.1"
                     /translation="CGAEDNALNYFQRSYDENKEYIFTRNRACMEFFNRHLDEPKILS
                     EPEYEDDRVCEPIVLSSWVSFFGDEAE"
BASE COUNT          202 a          139 c           96 g          181 t
ORIGIN      
        1 cctcttctgc aatccaggtt aaaaacgatg tatcggcgcc acctaattta acaacgcaat
       61 catataacat cataacacca aaatcatgtt cgatatccca ggagcatgag aactcgaaac
      121 caatataagg ctggccatta ttatgcaccg ataaaatatg tatcgctaat ggtgataaca
      181 actttgaaaa atcctcttga gttccaacat ctggcatata caactctttt tcttcctcgt
      241 catagtcata ttgcccttgt aaatcaggat attttttcat aagttccgaa agaatggatg
      301 ccaaaatttc agattgatga acaataatat actgtaaccc agtcttatgg cattcgctca
      361 tacatgaagt tggatcatcg ccaccaaggc tgtagtataa ttattcagcc tcatcaccga
      421 aaaaactcac ccaggatgat aagactattg gttcacagac tcggtcatcc tcatactctg
      481 gttccgacaa tattttaggt tcatcaagat gacggttaaa aaactccata catgcacggt
      541 tacgggtaaa gatatattct ttattttcat cataactacg ttgaaaatag ttaagcgcat
      601 tatcctctgc tccacacg
//
LOCUS       DAPGOF010000422          614 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.435, whole genome shotgun sequence.
ACCESSION   DAPGOF010000422 DAPGOF010000000
VERSION     DAPGOF010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.435"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>614
                     /locus_tag="RHV13_004852"
     CDS             <1..>614
                     /locus_tag="RHV13_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000183753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1244267.1"
                     /translation="LHNINQNSAVATKSSCQSIESIEKTITFNILDMLDNLTMRQQNI
                     MEAFLRVFTSMYNASAKADASMSIVFEQLVHLQSSETKNASWANLTGAIVGSAINVTG
                     AVFMAGAELAKPAGKIDDIAENQEQNAEKPEDQTRIRSKEDMANAKTRLAKIEEQQKE
                     LSKNQNIEDNDKQEQSSKLQKEKESITNKIKDKEKINSHNLKYG"
BASE COUNT          257 a          103 c          116 g          138 t
ORIGIN      
        1 cactacataa tataaatcaa aattctgctg tagcaacaaa atcatcctgt cagtctatcg
       61 agtcaataga gaaaaccata acatttaata ttctcgatat gttagataat ttgacgatga
      121 gacaacagaa cattatggag gcctttctaa gagtctttac atcaatgtac aatgcctctg
      181 ccaaggctga tgctagcatg agcatcgttt ttgaacagct tgtccatctt cagagtagcg
      241 aaacaaaaaa tgcttcttgg gcaaatttaa caggtgcaat agtaggctca gctattaatg
      301 ttacgggagc tgtatttatg gcaggtgcag aacttgcgaa gcccgcggga aagatagatg
      361 atatcgcaga aaaccaggaa caaaatgcag aaaaaccaga ggaccaaact cggataagat
      421 caaaagaaga tatggcaaat gcgaaaacca gacttgccaa aatagaagag caacaaaaag
      481 aattatcaaa gaaccagaat attgaggata atgacaaaca agagcaatca agtaagttac
      541 aaaaagaaaa agaaagtatc actaataaaa ttaaagacaa agaaaaaatt aatagtcaca
      601 atctaaaata tggt
//
LOCUS       DAPGOF010000423          612 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.436, whole genome shotgun sequence.
ACCESSION   DAPGOF010000423 DAPGOF010000000
VERSION     DAPGOF010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.436"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(27..611)
                     /locus_tag="RHV13_004853"
     CDS             complement(27..611)
                     /locus_tag="RHV13_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HDV1244268.1"
                     /translation="MQHNSLNAYYQQAFYDVIRNAVDEFPRTLALRVDLRFPRHYQYG
                     DSNKEVTRFIESLKAKLLVDCQRKNLRWKRNRSNRLRYAWVREVGELNRRKHYHVLLL
                     LNKDFYHGAGNYNSDDSLYALIQQAWCSALGLDTEQYTGLANMTENGCFYLNRKLPNY
                     MQQVKELLKRMEYMAKDHTKSYDDGYRSIGMSRR"
BASE COUNT          172 a          140 c          114 g          186 t
ORIGIN      
        1 aaacctgcca tctcacaacg cgcatgttac ctgcggctca ttccaattga tcgatatcca
       61 tcgtcataac tcttggtgtg gtctttagcc atatactcca tgcgcttcag taattcttta
      121 acctgttgca tataattagg gagttttctg tttaaataaa aacaaccgtt ctcagtcata
      181 ttcgccagac ctgtatattg ctctgtatcc aagcctaacg cactacacca tgcctgctgg
      241 attaatgcgt ataaactgtc atcggagtta tagttcccgg caccatggta gaagtcttta
      301 ttcagtagca gtaatacatg gtaatgttta cggcggttaa gttcacctac ttcacgtacc
      361 catgcatacc gtaatctatt agatcggtta cgtttccatc gtaggttctt acgttgacaa
      421 tcgaccagca acttagcttt cagtgattca ataaaacgag taacctcttt gttcgaatca
      481 ccatactgat agtggcgggg aaaacgtaaa tcaacacgta gtgctaacgt acgtggaaac
      541 tcgtctactg cattacgaat aacatcataa aatgcttgct ggtagtaagc attcaaggaa
      601 ttatgctgca tg
//
LOCUS       DAPGOF010000424          606 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.437, whole genome shotgun sequence.
ACCESSION   DAPGOF010000424 DAPGOF010000000
VERSION     DAPGOF010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.437"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            425..>606
                     /locus_tag="RHV13_004854"
     CDS             425..>606
                     /locus_tag="RHV13_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IpaD/SipD/SspD family type III secretion system
                     needle tip protein"
                     /protein_id="HDV1244269.1"
                     /translation="MATTKGTLTHVIASLDSFAPEMRSTATSSTLTASSSSEFKSDKY
                     ICSDIADLMSVLGSDYL"
BASE COUNT          212 a          120 c          125 g          149 t
ORIGIN      
        1 taaagaggca tcctcgcaaa gtgaggagcc tcatcaactg ggaaacagca aaagcgaggg
       61 taatttgcat aacagcgcca gtaaggataa taatattggc actatgctca agaacgatgt
      121 tgaaaaacta tcaactttag tcagtgaacg tagcccgcga aatgaagact ggaaatgcca
      181 gcacgacata caacttaact tcgttaaaaa catgctcaga aatttccctg ctgaaaatct
      241 gtcagagaaa ggatctgcac ttttacatca aagttattcg ctgataaaaa acgttctgca
      301 gcttacagaa aatgttaata ctattgacgg aaaattaaag gatcccgcta aaatatttgc
      361 cctgcataac tctgtgagaa aaacgctggc taacgttgct cacaacacaa ccgggacaag
      421 tgcaatggca acgaccaaag gaaccttaac tcacgttatc gcttctttag atagttttgc
      481 gcctgaaatg agaagtacag caaccagcag tactttaacg gccagtagtt caagcgaatt
      541 taaaagtgat aaatatatat gtagtgatat tgctgatttg atgagtgtgc tcggtagtga
      601 ctacct
//
LOCUS       DAPGOF010000425          600 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.438, whole genome shotgun sequence.
ACCESSION   DAPGOF010000425 DAPGOF010000000
VERSION     DAPGOF010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.438"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..474
                     /locus_tag="RHV13_004855"
     CDS             <1..474
                     /locus_tag="RHV13_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IpaD/SipD/SspD family type III secretion system
                     needle tip protein"
                     /protein_id="HDV1244270.1"
                     /translation="PDYVLYPPAPADKIGVPLEEAEKWCAALGLPVIPPDPKHRTPSP
                     IVEVEPQGSGLYVIIPNPQIIDSMSQSSDSMVHRDDKGKEKNISKEFTGYEISTAEYQ
                     AWLAGYNSQAENMKTDVQVITTKYSTANSTYDTIIKLLSSTITALFDSAKDYLRF"
BASE COUNT          186 a          141 c          113 g          160 t
ORIGIN      
        1 ccagactatg ttttataccc tccggcacct gctgacaaaa ttggtgttcc actggaagaa
       61 gcagaaaaat ggtgcgccgc tttaggactt ccggttattc cacccgaccc aaaacaccgc
      121 acaccttctc ctatagtcga agttgaaccg caaggtagcg gtctgtatgt tattattccc
      181 aaccctcaga ttattgacag tatgagtcag tcttcagact caatggtaca cagggatgat
      241 aaagggaagg aaaaaaatat atctaaagaa ttcaccggct acgaaataag cacggctgaa
      301 taccaggcat ggctggcggg ctataacagc caggcagaaa acatgaaaac cgacgtacag
      361 gttatcacga ctaaatacag tacagccaac tctacctacg atacgattat caaattatta
      421 tcttcgacca ttactgcgtt gtttgattca gcgaaggatt atttacgctt ttgattaagt
      481 aacacccaca attatcgtcc actatctggt cccgtaaaat gggaccagcg tttcatatta
      541 ttaagcatca gctttcattc atttcttcat cacaatctca caaaatgagc gttttttgat
//
LOCUS       DAPGOF010000426          596 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.439, whole genome shotgun sequence.
ACCESSION   DAPGOF010000426 DAPGOF010000000
VERSION     DAPGOF010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.439"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            2..451
                     /locus_tag="RHV13_004856"
     CDS             2..451
                     /locus_tag="RHV13_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001751863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HDV1244271.1"
                     /translation="MPYTFNQKILQGKVIRVLDGDTIEIKTLPAKIVVYEVPIRVRLI
                     NIDAPEKKQPFGRWSTSQLKTLVAGKQVTVSYSHKDRYGRIIGHVFTTNGTDASRFMV
                     QSGAAWVYERYNVDESLPALQREAQEQKRGLWADANPVPPWEWRIRN"
BASE COUNT          172 a          122 c          139 g          163 t
ORIGIN      
        1 aataccttat acatttaacc agaaaattct tcaggggaaa gttattcgcg ttctcgatgg
       61 cgacaccata gaaattaaaa cactacccgc aaaaattgtt gtgtatgaag ttccgatccg
      121 agtccgattg ataaacatcg atgcccctga aaagaaacaa ccttttggac gctggtcaac
      181 tagtcagctt aaaactctgg ttgcaggtaa acaggtgaca gtctcttatt cacataaaga
      241 ccgctacgga cgcattatcg gtcatgtgtt cacgacgaac ggcacggatg ccagccgctt
      301 tatggttcag tctggcgctg catgggtata tgaacgttac aacgtggatg agtcattacc
      361 ggctctgcaa cgggaagcgc aggaacaaaa acgtggtttg tgggccgatg caaatccggt
      421 tccaccgtgg gaatggcgta tcagaaatta agggggcaag aaaatgcatt cttcttttgg
      481 tttgccatga ttctgtcttt ttatctttgt gcaatattaa aggcttcttt ttaacagggg
      541 gcggcatgta tgtaattaat caacagacaa gggacaataa tattcttacg ttgcat
//
LOCUS       DAPGOF010000427          580 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.441, whole genome shotgun sequence.
ACCESSION   DAPGOF010000427 DAPGOF010000000
VERSION     DAPGOF010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.441"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            123..217
                     /locus_tag="RHV13_004857"
     tRNA            123..217
                     /locus_tag="RHV13_004857"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:157..159,aa:Sec,seq:tca)
     gene            296..457
                     /locus_tag="RHV13_004858"
                     /pseudo
     CDS             296..457
                     /locus_tag="RHV13_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280588.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
BASE COUNT          142 a          151 c          130 g          157 t
ORIGIN      
        1 gaactataat gctttcgtga taatactctg cgtgtattag gcggaaaaaa ctgatctggg
       61 ggatgtagaa actcaaggaa gtagctataa tgcgccccgc ctccatgtag caatcgaggc
      121 gcggaagatc gtcgtctccg gtgaggcggc tggacttcaa atccagttgg ggccgccagc
      181 ggtcccgggc aggttcgact cctgtgatct tccgccaaaa tacctcttct gacgttctcc
      241 aaccccttgt aatactgtca atccagagat tttctactgc tcaatattca atcggcttca
      301 ggaatacctg atcaaaggtt ttgtgatgga cgatgagcga ctgaagaatc cgcctgtcgg
      361 ttcttctgtc ctcgaacgca tccgcgatat tcgcgccagc atgcgccgtg tttatttgca
      421 ggtgatttgc tcccctgctg actctcagcc aactgtatcg ccattaaggt gaagatgttg
      481 ttttacacgc tccagaacac ccttttttat acgactattc tattccttta acagctggcg
      541 aaaaaaactg acctataaac ctcaccaata atcagactgc
//
LOCUS       DAPGOF010000428          575 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.442, whole genome shotgun sequence.
ACCESSION   DAPGOF010000428 DAPGOF010000000
VERSION     DAPGOF010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.442"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            121..>575
                     /locus_tag="RHV13_004859"
     CDS             121..>575
                     /locus_tag="RHV13_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1244272.1"
                     /translation="MIKVITLLLFALLTGCQSVESKIEELPTVGFDPILYNKSEAFTD
                     GKVTLRVESSGTDVWLVAKNGTRSFIELSGLTLGGSRCAYNARSKQLLPPGSVSTFVV
                     PTVGMLGLCFNNEDQLMFINRAFSRISPKAKGKDSLSLLFSVSYDFPGKA"
BASE COUNT          170 a          109 c          119 g          177 t
ORIGIN      
        1 atattgagaa aagaaaagat atcctcggtg gttatggtta ttggacgaaa gaaacaattc
       61 tctaccccgg tgaatcatta tcttttataa ccagcgccaa aaacaatcta aggaagcggg
      121 atgattaaag ttatcacact tttattgttt gcattactga ctggttgtca gtcagtagaa
      181 agtaaaatcg aagaactccc gacagttggt tttgacccta ttctatacaa taaaagtgag
      241 gcctttactg acggaaaagt tactttacgt gtcgaaagtt caggaactga tgtttggctt
      301 gtcgccaaga atggaaccag aagttttatt gagctttcag gcctaacgct aggtggtagt
      361 cgttgtgctt acaatgcccg tagtaagcaa cttttgcccc caggcagcgt aagcacattc
      421 gtagttccta cggttggaat gcttggactt tgcttcaaca atgaagatca acttatgttt
      481 attaaccgtg cttttagcag aatctctccc aaagcaaaag gaaaagattc tctttctctt
      541 ttattttctg ttagttacga ttttcccgga aaagc
//
LOCUS       DAPGOF010000429          573 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.443, whole genome shotgun sequence.
ACCESSION   DAPGOF010000429 DAPGOF010000000
VERSION     DAPGOF010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.443"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..>573)
                     /locus_tag="RHV13_004860"
     CDS             complement(<1..>573)
                     /locus_tag="RHV13_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDV1244273.1"
                     /translation="TELGSVDKGTTRTDNTLLERQRGITIQTGITSFQWENTKVNIID
                     TPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIPTIFFINKIDQ
                     NGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDTVIEGNDDLLEKYM
                     SGKSLEALELEQEESIRFQNCSLFPLYHGSA"
BASE COUNT          157 a          122 c           91 g          203 t
ORIGIN      
        1 tgcacttcca tgataaagag ggaacagaga acaattctga aatcttatgc tttcctcttg
       61 ttcgagttcc aatgcttcta atgatttacc ggacatatat ttctctaaaa ggtcatcgtt
      121 tccctctatt accgtatccc attgttcaga ttcggtaaag ttcgtcacac acatattagg
      181 atacagttct accttctgtt tgattacaat ttcggcagaa agtttctctt taatatcctg
      241 ataaaccgtt gataaatcaa ttccattttg gtcaatctta ttgataaaaa agattgtggg
      301 aatccccatt ttcctaagtg catgaaataa tatacgagtt tgtgcttgta cgccatcttt
      361 tgcagaaatc agtagaattg ccccatctaa aactgataat gaacgatata cttctgctaa
      421 gaaatccata tgtcctggcg tgtctatgat gttcaccttc gtattttccc actgaaaaga
      481 ggttattcct gtctgaattg taattcctct ctgacgttct aaaagcgtat tatccgtcct
      541 cgttgtacct ttgtccacgc ttcctaattc tgt
//
LOCUS       DAPGOF010000430          572 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.444, whole genome shotgun sequence.
ACCESSION   DAPGOF010000430 DAPGOF010000000
VERSION     DAPGOF010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.444"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..554)
                     /locus_tag="RHV13_004861"
     CDS             complement(<1..554)
                     /locus_tag="RHV13_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HDV1244274.1"
                     /translation="MENNDKFLSQDLLESYAIRLLSGPLNGCEYEILNGRLLVIIGND
                     VSLGKSDAFSELPENTIVVPYGELTGSFEIIITTDPDLVVTIRELTAQEPEDRTLTLN
                     QQIEVLGLKFAVKEKNEVWQYSLPGIIENNIISTEQRFFSSKLFKYVMLFFLFAIIFI
                     SFYIVNASNDPQLRHIDKILVNKN"
BASE COUNT          195 a          106 c           83 g          188 t
ORIGIN      
        1 ctgtttttgt ttacaagaat tttatcgata tgtctcagct gcggatcatt actggcatta
       61 acaatataaa aagaaatgaa aatgatagca aaaagaaaaa aaagcattac atacttaaac
      121 agcttactgc taaagaaacg ttgttctgta gagataatgt tattttcaat aatgcctggc
      181 aatgaatact gccaaacttc atttttttct tttacagcaa atttgagacc taaaacttct
      241 atttgctgat tcaatgttaa cgttctatct tcaggttctt gagctgttaa ttctctgatt
      301 gttacaacca gatcagggtc ggtagtaatt attatctcaa aactacctgt aagctcgcca
      361 taaggaacga ctattgtatt ttctggtaac tcagaaaaag catcactttt tcctaacgaa
      421 acatcattac caatgataac aagtagacgt ccattaagta tttcatactc gcatccattc
      481 aatggtccgc tcaacaaacg aatggcataa gattccaata agtcttgtga taagaattta
      541 tcattatttt ccatgaatac cacccgctaa tt
//
LOCUS       DAPGOF010000431          544 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.445, whole genome shotgun sequence.
ACCESSION   DAPGOF010000431 DAPGOF010000000
VERSION     DAPGOF010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.445"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            2..>544
                     /locus_tag="RHV13_004862"
     CDS             2..>544
                     /locus_tag="RHV13_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1244275.1"
                     /translation="MGAVELFVKSSYTKTHSNNLFNNIHHAFKKKDWISNYDSLLTLR
                     EFFRCATQIDKSSYQVLSSKNETVNAMDKFLISFSLKDNGAEYTMTLRGSGFEYEEIP
                     ITINEYNSFMDFKNREFPLEQNRRLYAWDILQKKQSDIPKRIKGYIHQAIGDVCLGYA
                     LLEDIVSKLKRGKFELQIPGG"
BASE COUNT          192 a           83 c          108 g          161 t
ORIGIN      
        1 tattggtgcg gtggagttat ttgtaaagtc ttcgtataca aaaacacatt caaacaatct
       61 ttttaacaac atccatcatg catttaagaa aaaagattgg atttcgaatt atgatagcct
      121 gttaactttg cgggagttct ttcgttgcgc aactcagatt gataaaagta gttatcaggt
      181 gctttcatca aaaaatgaaa ctgttaacgc catggataag tttcttatca gtttcagtct
      241 taaagataac ggtgccgaat atacaatgac attgcgaggt agtggatttg aatacgaaga
      301 aatacccata acaataaatg aatataactc attcatggat ttcaaaaacc gtgaatttcc
      361 gttagaacaa aacagacgat tgtatgcctg ggatattcta cagaaaaaac agtctgacat
      421 accgaaaagg attaaaggtt atattcacca ggcgattggt gacgtgtgtt taggttatgc
      481 cttattggag gatatagtaa gcaaattaaa acggggtaaa tttgaactcc aaataccagg
      541 aggt
//
LOCUS       DAPGOF010000432          539 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.446, whole genome shotgun sequence.
ACCESSION   DAPGOF010000432 DAPGOF010000000
VERSION     DAPGOF010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.446"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..>539)
                     /locus_tag="RHV13_004863"
     CDS             complement(<1..>539)
                     /locus_tag="RHV13_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HDV1244276.1"
                     /translation="SHSFVFHGTGERYFLICVVNVLLTIITLGIYLPWALMKCKRYLY
                     ANMEVNGQRFSYGITGGNVFVSCLVFVFCYFAILMTVSADMPLVGCVLTLSLLVLLIF
                     MAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTVFFISTKMLHAN
                     SSSSVIISVVLMAIVGIVS"
BASE COUNT          217 a          127 c           77 g          118 t
ORIGIN      
        1 tggaaacaat accaactatt gccatcagaa ccacagatat aataacacta cttgaactat
       61 tggcatgtag catctttgtc gagataaaga aaacagtccc catcccaatg gccattaaaa
      121 tcggcaagaa aaaggttacc caccagaacc ctttcaaaga gcaattaaaa ctaaatctta
      181 cgccgttgag actggtcatc aaggcctgat aacgcagtcc ttttgctgcc ataaatataa
      241 gcaaaaccaa cagtgacaaa gtcaaaacac agccaacaag cggcatatct gctgacactg
      301 tcattaagat tgcgaaatag caaaaaacaa aaacaagaca actaacaaaa acattcccac
      361 cagtaattcc ataagaaaat cgttgtccgt taacttccat attagcatag agataacgct
      421 tacatttcat taatgcccat ggcaaataga tacctagcgt tataatcgtt aacaacacat
      481 tcaccacaca aataagaaaa tatctctcac ctgtaccatg aaaaacaaac gaatgggac
//
LOCUS       DAPGOF010000433          534 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.447, whole genome shotgun sequence.
ACCESSION   DAPGOF010000433 DAPGOF010000000
VERSION     DAPGOF010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.447"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            87..335
                     /locus_tag="RHV13_004864"
     CDS             87..335
                     /locus_tag="RHV13_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1244277.1"
                     /translation="MFIYASGGNGGSAGGACANTSRLQGYVGGTLISVNASNNPAYGK
                     TAFISFAVPAGTSYQITSYPTENTSCGAGVFSVFGYQT"
     gene            complement(332..>534)
                     /locus_tag="RHV13_004865"
     CDS             complement(332..>534)
                     /locus_tag="RHV13_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1244278.1"
                     /translation="TIGGKLKVTQLSTTGYLGQFDFCAIARMGNAEDAHYCQVVESPA
                     GSRKWYKYEHKTGCIASCVTLN"
BASE COUNT          136 a          119 c          126 g          153 t
ORIGIN      
        1 agacttctgg ttcgctcaat ggttcttaca caaacttagg ttcacataga ggttcattct
       61 cagggcggaa ttcagggggc agtacattgt ttatttatgc atctggaggt aatggaggat
      121 ctgccggagg tgcatgtgca aatacatccc gactgcaggg atatgttggt gggacgctaa
      181 ttagcgtgaa tgccagcaat aaccctgcat atggaaaaac agcctttatc agctttgctg
      241 tacctgcagg tacttcctat cagataacat cctatccaac agaaaataca tcatgtggtg
      301 ccggggtatt ttcagtattt ggatatcaaa cttaattgag cgttacacac gacgcaatac
      361 aacctgtctt atgctcgtat ttgtaccatt tgcgtgaacc tgctgggctc tcaactacct
      421 ggcagtagtg ggcatcctct gcgttgccca ttctggcaat ggcacagaag tcgaattgcc
      481 ccagataacc tgtggtggaa agctgagtaa ctttgagttt tccacctatt gtcc
//
LOCUS       DAPGOF010000434          508 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.448, whole genome shotgun sequence.
ACCESSION   DAPGOF010000434 DAPGOF010000000
VERSION     DAPGOF010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.448"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
BASE COUNT          130 a          113 c          126 g          139 t
ORIGIN      
        1 agaaaaatgg cggcggtaca gtacagatgg ttactgcgac agcaagtgac tggaatcagc
       61 caattgccac cgcgacttgc ccttcaggaa agaaagttac aggaggtggt ggaatgtgca
      121 gcttttcaaa tgccattctc aagcgttctt ccccctccgg taacagtgca tgggtagctg
      181 gttgcagcca aaacaccaac cagaatcagt attattcagt cactacttat gcattgtgtc
      241 agtgagtatt ctggtatgaa ttagcggaag cagtgaacag tgatgtagta gctgccatat
      301 aacgtaccgt tatacgcact ccagttcatc tcgccgtttg catctggatt gtctgtaggc
      361 cagacacgcg tcatcgcggt ttcctttcca tctatgcgat atgtattaac gcaaagtttg
      421 aattttccta gagccttcgt tgtggttggg tacaaggtga aggatgttac tgatgtccac
      481 ggcgcagatg aacctattga tttccacg
//
LOCUS       DAPGOF010000435          504 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.449, whole genome shotgun sequence.
ACCESSION   DAPGOF010000435 DAPGOF010000000
VERSION     DAPGOF010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.449"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..350)
                     /locus_tag="RHV13_004866"
     CDS             complement(<1..350)
                     /locus_tag="RHV13_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HDV1244279.1"
                     /translation="MANKTEKPTQKKLQDASKKGQILKSRDLTVSVIMLVGTLYLGYV
                     FDVHHIMSILEYILDHNAKPDIWDYFKAMGIGWLKTIIPFLLVCMFTTILVSWFQSKM
                     QLATEAVKFKFDSL"
BASE COUNT          174 a           91 c           81 g          158 t
ORIGIN      
        1 ttcaatgaat caaacttaaa tttaacagct tcagttgcta attgcatttt actttgaaac
       61 caggagacaa gtattgttgt gaacatgcaa accagcaaaa aaggaatgat cgttttcaac
      121 caaccaatcc ccatagcttt aaaatagtcc caaatatctg gtttagcgtt atgatcaagg
      181 atgtattcaa gaatcgacat aatgtgatgc acatcaaaaa catatccgag atataaagta
      241 cccacaagca ttattacaga gacggttaag tctctacttt ttagaatttg ccctttttta
      301 gaagcatcct ggagtttctt ttgtgttggc ttttcagttt tatttgccat gaaacactct
      361 atttctcaat atatagttgc aattgttccg gaaaaaaaga taacggcatc actcgttcag
      421 ccaatattgg gccaaaataa ataattaata ttaaaaatgc cagcgcactt ttgacagtca
      481 gtgaaatggc aaaagcattt agtt
//
LOCUS       DAPGOF010000436          492 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.451, whole genome shotgun sequence.
ACCESSION   DAPGOF010000436 DAPGOF010000000
VERSION     DAPGOF010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.451"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            93..284
                     /locus_tag="RHV13_004867"
     CDS             93..284
                     /locus_tag="RHV13_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HDV1244280.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT          127 a          113 c           91 g          161 t
ORIGIN      
        1 ttttttacat aacgaagaat tgaccatttt gtcctgttgt gccttaatgt aagtaccgtc
       61 cacggcgtgg gacatacttc aaggaacctt ttgtgagtca ggcaaccagt atgcgaaaac
      121 gacaccgatt taacagtcgc atgacccgta tcgtactgct catcagcttt atcttcttct
      181 ttggccgttt tatctactcg tccgtcggtg cctggcagca ccatcagagc aaaaaagaag
      241 ctcagcaatc cacactctcc gtcgaatcac cggtacaacg ttagcggtta ccttctccac
      301 tttcacagaa cataacggca cttcgctgtc ggatgctttt gctctttggg attatcaaag
      361 cggcagatat tctttcatct taaattttac gtctttgtcc tgactgatgt ttatcctgtt
      421 tggctgcgaa ataaatataa aattaatata tatgttgtaa tgatatattt ttataaatta
      481 ttccctgcgt ga
//
LOCUS       DAPGOF010000437          491 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.452, whole genome shotgun sequence.
ACCESSION   DAPGOF010000437 DAPGOF010000000
VERSION     DAPGOF010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.452"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..109)
                     /locus_tag="RHV13_004868"
     CDS             complement(<1..109)
                     /locus_tag="RHV13_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDV1244281.1"
                     /translation="MTNHIHFRCPCCHGSQYRTSSFDVSDRNPFGAKCIF"
     gene            complement(120..332)
                     /gene="cspI"
                     /locus_tag="RHV13_004869"
     CDS             complement(120..332)
                     /gene="cspI"
                     /locus_tag="RHV13_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspI"
                     /protein_id="HDV1244282.1"
                     /translation="MSNKMTGLVKWFNPEKGFGFITPKDGSKDVFVHFSAIQSNDFKT
                     LTENQEVEFGIENGPKGPAAVHVVTL"
BASE COUNT          173 a           99 c           83 g          136 t
ORIGIN      
        1 aaaagataca ttttgccccg aaaggatttc tgtcagaaac atcaaaagat gatgttctat
       61 actgagagcc atggcaacac gggcatctaa agtgaatatg gtttgtaata ttttctacct
      121 caaagtgtca ctacatgaac agctgcaggg cctttaggtc cgttctcaat accaaattcg
      181 acttcctgat tctcagttaa cgttttgaaa tcgttgctct gaatagctga gaaatgcaca
      241 aacacatctt tgctaccatc tttcggcgtg atgaaaccaa aacctttttc agggttaaac
      301 catttcacta aaccagtcat tttgttagac atcattatta ccttttgaag aagttagccc
      361 ttgggcagaa tggtccgaaa aaaaatatca gagagaaaaa ccaacaagga aatctcaaga
      421 ggtacaaata ataaaattat aacaatgact gcttcagata attttgtaac aaaccagaac
      481 accattaacg c
//
LOCUS       DAPGOF010000438          491 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.453, whole genome shotgun sequence.
ACCESSION   DAPGOF010000438 DAPGOF010000000
VERSION     DAPGOF010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.453"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(110..>491)
                     /locus_tag="RHV13_004870"
     CDS             complement(110..>491)
                     /locus_tag="RHV13_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1244283.1"
                     /translation="NDGNTIDTEPKPPINVLLENVLDQKFKEYLTPLQLDNSKQDSVS
                     VKETFLVQKEHACFGVKIENEGSDTSIPSESPLSSDASKISKEKSISAVVPVLEKVSD
                     ENQTASISIKSKAKANKRLATLAR"
BASE COUNT          136 a           93 c           96 g          166 t
ORIGIN      
        1 gacccaggtg aagcctgaaa ttattataac aaaaaaaaag gccagcaagt atgctggctt
       61 tcctggtaag acaagttgga actcagaagt cgattgtact gtctctcacc tatcttgcca
      121 aagttgccag tcgcttgttt gccttagctt tagattttat gcttatggag gcggtttgat
      181 tttcatccga tactttttct agcactggca ccacagcgga aatggacttt tcttttgaaa
      241 ttttggatgc atctgaagaa agtgggcttt cagatggtat agaggtatcg cttccctcat
      301 tttcaatctt cacaccaaag caggcatgtt ccttttgtac aaggaaggtt tcttttacag
      361 aaacagaatc ttgctttgaa ttatctaatt gtagaggtgt taaatactcc ttaaactttt
      421 gatctaagac attctctagc aatacattaa taggaggctt aggttcagta tctatagtat
      481 ttccatcatt c
//
LOCUS       DAPGOF010000439          485 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.454, whole genome shotgun sequence.
ACCESSION   DAPGOF010000439 DAPGOF010000000
VERSION     DAPGOF010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.454"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>485
                     /locus_tag="RHV13_004871"
     CDS             <1..>485
                     /locus_tag="RHV13_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311760.1"
                     /note="may be involved in the export or assemby of
                     proteins involved in the entry of Salmonella into host
                     cells; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscC/YscC/HrcC family type III secretion system
                     outer membrane ring protein"
                     /protein_id="HDV1244284.1"
                     /translation="GNGTFYVSGPPVYVDLVVNAAKLMEQNSDGIEIGRNKVGIIHLV
                     NTFVNDRTYELRGEKIVIPGMAKVLSTLLNNNIKQSTGVNVLSEISSRQQLKNVSRMP
                     PFPGAEEDDDLQVEKIISTAGAPETDDIQIIAYPDTNSLLVKGTVSQVDFIEKLVATL
                     D"
BASE COUNT          159 a           85 c          109 g          132 t
ORIGIN      
        1 ggcaatggta ctttctatgt atcggggcca ccggtttatg ttgacctggt cgttaatgct
       61 gcgaaattaa tggagcaaaa cagcgatggc attgagatcg gtcgaaataa agtaggaata
      121 attcatctgg tcaacacatt cgttaatgac cggacttatg agttacgtgg cgaaaagata
      181 gttattcctg gcatggcaaa agtactatcg acgttgttaa ataataacat taagcaaagc
      241 acaggggtga acgtactttc tgaaatttca agtcgtcaac aactaaaaaa cgtatcacgt
      301 atgcctcctt ttcctggggc tgaagaagat gatgacctac aagtagagaa aataatatcg
      361 acagcaggag cgccagagac tgacgatata caaattattg catatcctga taccaacagt
      421 ttacttgtta aaggaacagt atctcaggtt gattttatcg agaagttagt agctacgctt
      481 gatat
//
LOCUS       DAPGOF010000440          467 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.455, whole genome shotgun sequence.
ACCESSION   DAPGOF010000440 DAPGOF010000000
VERSION     DAPGOF010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.455"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..>467)
                     /locus_tag="RHV13_004872"
     CDS             complement(<1..>467)
                     /locus_tag="RHV13_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HDV1244285.1"
                     /translation="TVGLEPYNQDDDISLGIINKLDQVITETVPLVLIMNSVQAKKYT
                     EINLADRIRSQFFIEYGIRIPGIVIREGEGLNDEDVILMLNEVRASQFKIYHDLVLLV
                     EYSDEVVSTLIKKPVIVNSNGEQYYWVTKSDAQKLTKIGCYTRTAMDEMYNHLS"
BASE COUNT          154 a           98 c           67 g          148 t
ORIGIN      
        1 gataaatgat tatacatttc atccatggcc gtgcgagtat aacagccaat ttttgttaat
       61 ttttgagcat cacttttcgt aacccaataa tattgttcac cattactatt tacaatgaca
      121 ggtttcttga tcaaagtcga aaccacttca tctgaatatt caaccaacaa gacaagatca
      181 tgataaattt taaattgcga agctctgact tcatttagca ttaatatgac atcttcatca
      241 ttaagacctt ccccctcacg aatgacaata ccgggaatgc ggataccata ctcaataaaa
      301 aactgactac gaatcctatc agccagattg atttcagtgt attttttagc ctgtacacta
      361 ttcataatca agaccaaggg taccgtttcg gtaataacct gatcaagttt attaatgata
      421 cctaatgaga tgtcatcatc ttgattatag ggttccaacc caacggt
//
LOCUS       DAPGOF010000441          452 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.456, whole genome shotgun sequence.
ACCESSION   DAPGOF010000441 DAPGOF010000000
VERSION     DAPGOF010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.456"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..76
                     /locus_tag="RHV13_004873"
     CDS             <1..76
                     /locus_tag="RHV13_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HDV1244286.1"
                     /translation="LEQLINVNFFSSDRTSFCECNRFP"
BASE COUNT          142 a           90 c           91 g          129 t
ORIGIN      
        1 tctcgaacaa ttgattaacg tcaacttttt ctcttctgac aggacgtcat tttgtgaatg
       61 caatcgtttt ccataaattc ttctcccctc ataggcgacg aatagcattt tgtgttgagg
      121 atcacaaaac gaataattgc tgatcgccgc gataaggtca gacaaagaca acaaaggaaa
      181 ttttcacaga gcttttgatc ggcgtaggcc acagaatgtt gcttcgttta ctgtaacgcc
      241 gggtaaatga gcgttttttg atagtgcgaa aaaaccctgc gaggaaaata agcatctatt
      301 attgtttgtc atgacgacaa ggcaggcatc aatgaaaggc ttcccaattg cacatatttt
      361 tcacccttca atcccgccaa tgcacgcagt ggttaacaat cacaatagaa atattgatta
      421 ttggacggta aaaagaaagt ttgcagaaat tg
//
LOCUS       DAPGOF010000442          446 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.457, whole genome shotgun sequence.
ACCESSION   DAPGOF010000442 DAPGOF010000000
VERSION     DAPGOF010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.457"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            171..>446
                     /locus_tag="RHV13_004874"
     CDS             171..>446
                     /locus_tag="RHV13_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HDV1244287.1"
                     /translation="MDCSKCNGYATMLLNMVQGSDPVNLLELHGFLEHFAYYVSFGKF
                     NAGHQRYNAFKKFVSEISEISANDINMTIKTGQSRHENVISINMNDAI"
BASE COUNT          141 a           76 c           95 g          134 t
ORIGIN      
        1 gatagaaaga ttgatcgcca gtctgtttag agcggtgcag cagcaaactt tcataaccag
       61 gttaatcctg agtttgcagt acgaaaagtt aaaaatgagc gatttctgta agattcccca
      121 ttttgccagg cttatgattc gcagatcatt caagtgcaaa ggagcgatgt atggattgtt
      181 caaaatgcaa tggttatgcg acgatgttat taaatatggt gcagggttct gacccggtta
      241 atttactgga attacatggt ttccttgaac atttcgcata ttatgtctca tttggcaaat
      301 ttaatgctgg ccaccagcgc tataatgcat ttaaaaaatt cgttagtgag atttcggaga
      361 tcagcgcaaa cgatatcaat atgacgatta aaaccgggca atccagacat gaaaatgtta
      421 tctctataaa tatgaacgat gcaatt
//
LOCUS       DAPGOF010000443          437 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.458, whole genome shotgun sequence.
ACCESSION   DAPGOF010000443 DAPGOF010000000
VERSION     DAPGOF010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.458"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(114..320)
                     /locus_tag="RHV13_004875"
     CDS             complement(114..320)
                     /locus_tag="RHV13_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HDV1244288.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
BASE COUNT          120 a          106 c           85 g          126 t
ORIGIN      
        1 atatatgttt cgattggtat aacggtgtct aaatattcct ctgaacatct tccttataca
       61 aaaaaccgcc agcgtttccg ctggcggttt tcaatgtatt caaaactcag caatcagtaa
      121 aaatcaccag ttgccttttc ggcctgatcc atccacaccg gtttatcgct ggttttcgac
      181 cagacgcgat gcaggtagct gtaaaagcgg gcgcggtctt tccagaaaag catgaaaggc
      241 agcgccagaa cgcccgctgc aaccgcataa gtacggcgta aaaagataag ccatgcagga
      301 tattctctgt agagttccat acttttctcc cccttttgta tctacccggt gaatagcacc
      361 ggaaaaatga atttgtttat ctgatgaaaa tagtaccgcc ttttgtgtaa ttttactact
      421 catccgacca cttattt
//
LOCUS       DAPGOF010000444          399 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.denovo.461, whole genome shotgun sequence.
ACCESSION   DAPGOF010000444 DAPGOF010000000
VERSION     DAPGOF010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.denovo.461"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..>399)
                     /locus_tag="RHV13_004876"
     CDS             complement(<1..>399)
                     /locus_tag="RHV13_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDV1244289.1"
                     /translation="IAKDAVTNNIALMSTFYDSDNTSGEVLNNDFTEEESFETALDVE
                     HAIVLGFADDNFETKPVHYQDLLTRFSAFQDTVFNLFPEMHSSHYNDICSVSVDMTKG
                     TQCMIHLMVNEEVFMSLPVPVATMVREDAT"
BASE COUNT          128 a           85 c           61 g          125 t
ORIGIN      
        1 tagttgcatc ttcacgtacc atcgtggcaa caggaacagg caatgacatg aaaacttctt
       61 cattcaccat caagtgaatc atgcattgcg ttccctttgt catatccaca cttacgctac
      121 aaatatcgtt gtaatgcgaa gagtgcattt caggaaacag attaaacacg gtatcttgaa
      181 aggcactaaa tcgtgttaat aaatcctgat aatgaacagg ttttgtttca aaattatcat
      241 cagcaaatcc aagcactatt gcatgctcta catctaacgc agtttcaaat gactcttctt
      301 ctgtaaaatc attatttaaa acctcacctg acgtattatc actgtcataa aaagtgctca
      361 tcaatgcgat attgtttgtt acagcgtctt ttgcaatat
//
LOCUS       DAPGOF010000445        17549 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.guided.099, whole genome shotgun sequence.
ACCESSION   DAPGOF010000445 DAPGOF010000000
VERSION     DAPGOF010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.guided.099"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(251..796)
                     /gene="orn"
                     /locus_tag="RHV13_004877"
     CDS             complement(251..796)
                     /gene="orn"
                     /locus_tag="RHV13_004877"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HDV1244290.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            891..1943
                     /gene="rsgA"
                     /locus_tag="RHV13_004878"
     CDS             891..1943
                     /gene="rsgA"
                     /locus_tag="RHV13_004878"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HDV1244291.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMEFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            2040..3008
                     /gene="psd"
                     /locus_tag="RHV13_004879"
     CDS             2040..3008
                     /gene="psd"
                     /locus_tag="RHV13_004879"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HDV1244292.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            3030..6353
                     /gene="mscM"
                     /locus_tag="RHV13_004880"
     CDS             3030..6353
                     /gene="mscM"
                     /locus_tag="RHV13_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HDV1244293.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQISQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEIIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(6504..8006)
                     /gene="yjeM"
                     /locus_tag="RHV13_004881"
     CDS             complement(6504..8006)
                     /gene="yjeM"
                     /locus_tag="RHV13_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HDV1244294.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(8225..9202)
                     /gene="epmA"
                     /locus_tag="RHV13_004882"
     CDS             complement(8225..9202)
                     /gene="epmA"
                     /locus_tag="RHV13_004882"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HDV1244295.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEAYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9527..11335
                     /gene="frdA"
                     /locus_tag="RHV13_004883"
     CDS             9527..11335
                     /gene="frdA"
                     /locus_tag="RHV13_004883"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HDV1244296.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            11328..12062
                     /gene="frdB"
                     /locus_tag="RHV13_004884"
     CDS             11328..12062
                     /gene="frdB"
                     /locus_tag="RHV13_004884"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HDV1244297.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            12073..12468
                     /gene="frdC"
                     /locus_tag="RHV13_004885"
     CDS             12073..12468
                     /gene="frdC"
                     /locus_tag="RHV13_004885"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HDV1244298.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            12479..12838
                     /gene="frdD"
                     /locus_tag="RHV13_004886"
     CDS             12479..12838
                     /gene="frdD"
                     /locus_tag="RHV13_004886"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HDV1244299.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            12901..14034
                     /locus_tag="RHV13_004887"
     CDS             12901..14034
                     /locus_tag="RHV13_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HDV1244300.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLHFYQNWQPVWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMHTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQINLKPLDINEKTLQQGIQVAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            14123..14656
                     /gene="blc"
                     /locus_tag="RHV13_004888"
     CDS             14123..14656
                     /gene="blc"
                     /locus_tag="RHV13_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HDV1244301.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(14653..14970)
                     /gene="sugE"
                     /locus_tag="RHV13_004889"
     CDS             complement(14653..14970)
                     /gene="sugE"
                     /locus_tag="RHV13_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDV1244302.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(15145..15291)
                     /gene="ecnB"
                     /locus_tag="RHV13_004890"
     CDS             complement(15145..15291)
                     /gene="ecnB"
                     /locus_tag="RHV13_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HDV1244303.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(15402..15527)
                     /gene="ecnA"
                     /locus_tag="RHV13_004891"
     CDS             complement(15402..15527)
                     /gene="ecnA"
                     /locus_tag="RHV13_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HDV1244304.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(15579..16145)
                     /gene="efp"
                     /locus_tag="RHV13_004892"
     CDS             complement(15579..16145)
                     /gene="efp"
                     /locus_tag="RHV13_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDV1244305.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            16187..17215
                     /gene="epmB"
                     /locus_tag="RHV13_004893"
     CDS             16187..17215
                     /gene="epmB"
                     /locus_tag="RHV13_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HDV1244306.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVITSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRAGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         4376 a         4603 c         4514 g         4056 t
ORIGIN      
        1 ccttggcaag gtcgtgctct accaactgag ctattcccgc gtactactta attttgcttt
       61 cgtaattttc gcatttctgc gtcgttacgg gaggcgcatt atacgagaaa tctttccctc
      121 tgcaaccccc ctaaaaaatt ttttttgagt ttttatctca agtgctgaaa ttatcagcaa
      181 ggcgttcaat ttagcgtcaa aatcacgccg ccgcaagtgg attccgacgc ttacgtgacc
      241 aggaaaaatt ttacagcttg ataaaatgct cgcggtagta agccagctcc gccaccgatt
      301 cacggatatc atccatcgcc tgatgcgtcc cctgcttggt aaaaccatcc aaaatttccg
      361 gcttccagcg gcgcgccagc tctttcagtg tgctgacatc gagataacgg tagtggaagt
      421 aggcttccag ctccggcatg tatttaaaca ggaagcgacg atcctggccg atgctgttac
      481 cgcaaatcgg cgatttaccc gcaggcaccc actgttttaa aaattcgagc gttgccagtt
      541 cagcttcacg atcgcccatc gtgctcgctt tcacgcgctc taccagcccg ctggcggtat
      601 gggtgcgcac gttccagtca tccatcagcg ccagctgttc atcggactga tgtactgcaa
      661 tggtcggccc ttctgccaga atattcaggt tggcatcggt caccagcgtg gcaatctcaa
      721 taatgcgatc gcgctcggga tccagaccgg tcatctcaag atcgatccaa atcaggttgt
      781 tttcattggc actcatgcta ttttccaccc attcacagtg actatattga tccacaattg
      841 cgtgtatcat agatgttttg cccatcaggg gcgaccagga gtctgtacga ttgagtaaaa
      901 ataaactctc caaaggccag cagcgccgcg tgaacgccaa tcaccagcgt cgtcttaaaa
      961 cgtctaagga gaagcccgac tacgacgaca atctgtttgg cgagcctgat gaaggtatcg
     1021 tcatcagccg ctttggtatg cacgctgatg tggaatccgc cgatggcgac gttcaccgct
     1081 gcaatattcg ccgcaccatc cgttcactgg taaccggcga ccgcgtagtc tggcgtccgg
     1141 gtaaaccggc ggcggaaggc gtaaatgtca aagggatcgt ggaagcggtg catgagcgca
     1201 cctcggtgtt aacacgcccg gatttctatg acggcgtgaa acccatcgcc gccaacatcg
     1261 accagattgt cattgtctcc gccattttgc cggagctgtc actcaatatt atcgaccgtt
     1321 acctggtggc ctgcgaaacc ttgcagattg agccgattat tgtgctcaac aagatagacc
     1381 tgctggacga cgaaggtatg gagttcgtca acgagcagat ggatatctac cgcaatatcg
     1441 gttatcgcgt attgatggtt tccagccata ctcaggatgg gctaaaaccg ctggaagagg
     1501 cgttgaccgg gcgcatcagc atttttgccg ggcagtctgg cgttggtaaa tccagcctgc
     1561 tgaacgcgtt gctggggctg caaaaagaga tcctgaccaa cgatgtctct gacaactcgg
     1621 ggctcggtca gcacaccacg accgccgctc ggctgtatca cttcccgcac ggcggtgatg
     1681 tgattgattc ccctggtgtg cgtgagttcg gcctctggca cctggagccg gaacaaatca
     1741 ctcagggctt tgtcgaattc catgactatt taggtctgtg taaatatcgc gattgcaaac
     1801 acgataccga tccgggctgc gctatccggg aagcggttga ggaagggaaa atcgcggaaa
     1861 cccgtttcga aaactatcac cgtattctgg aaagcatggc gcaggtaaaa acgcgtaaaa
     1921 acttttctga tacggatgac tgacaattaa gctaaccgtc gttagaatcg tccccttttt
     1981 ttcaggatcc ggcatgtagg ccggatcagg aacgacaaaa caatggcctg gaggctacct
     2041 tgttaaattc atttaaactt tcgctacagt acattctgcc gaaactatgg cttactcgcc
     2101 tggcgggttg gggcgcaagc aagcgggcag gatggctgac aaaactggtt atcgatctgt
     2161 tcgttaaata ctacaaggtc gacatgaaag aggcgcaaaa gccggacacc gccagctacc
     2221 gcacctttaa cgaattcttt gtccgtccgc tgcgtgacga agtacgccca atcgataccg
     2281 atccgaatgt cctggttatg cctgccgatg gcgttatcag ccagttgggt aaaatcgaag
     2341 aagataaaat cctgcaagcc aaaggccaca actacagcct cgaagccctg ctggcaggca
     2401 actatctgat ggcggacctg ttccgcaacg gtacgtttgt gaccacttac ctctccccgc
     2461 gtgactacca ccgcgtacac atgccgtgca acggtattct gcgtgagatg atctacgtgc
     2521 cgggcgatct cttctccgtt aaccatctca cggctcagaa cgtgccgaat ctgtttgccc
     2581 gtaacgaacg cgtgatctgc ctgttcgata ccgaatttgg cccaatggcg cagattctgg
     2641 tcggtgcgac gattgtaggc agcattgaga ctgtctgggc gggcaccatt acgccgccgc
     2701 gcgaaggcat catcaagcgc tggacctggc ctgccgggga aaacgacggt tctgtggcac
     2761 tgctgaaagg ccaggaaatg ggtcgtttta aactcggttc caccgttatc aacctgtttg
     2821 caccgggtaa agttaatctg gttgagcaac tggaaagcct gtctgtcacg aaaattggcc
     2881 agccgctggc agtatctacc gaaacctttg ttacgccaga cgctgaacca gctccacttc
     2941 ctgctgaaga gatcgaagca gaacacgacg ccagcccatt ggttgacgat aaaaaagacc
     3001 aggtctaatc catcaaagga aacgctgacg tgcgcctgat tatcactttt ctgatggcct
     3061 ggtgcctcag ttggggggcg tacgccgcga cggcccccga tagcaaacaa atctcacaag
     3121 aactggagca ggcaaaagcg gcgaaacccg cacagccgga agtcgtagag gcgctccagt
     3181 ctgccttaaa tgcgcttgag gaacgaaaag gttcccttga gcgcatcaag caatatcaag
     3241 aaatcattga taattatccg aaactctccg ctactctgcg cgcacaatta aacaacatgc
     3301 gtgacgagcc gcgcagcgtg tcgccgggga tgtctaccga cgcgctgaat caggaaattc
     3361 tccaggtcag cagccagttg ctggataaaa gccgtcaggc ccagcaagag caggagcgcg
     3421 cccgcgagat tgccgattcg ctgaatcaac tgccgcaaca gcaaaccgac gcccgccgtc
     3481 agttaaatga gatcgagcgc cgcctgggaa cgcttactgg caataccccg ctcaatcagg
     3541 cacaaaattt cgcgttgcag tctgactccg cacgcctgaa agcgctcgtt gatgaactgg
     3601 aactggcgca gctgtctgcc aataaccgcc aggaattagc gcgcttgcgc tctgagctgg
     3661 cggaaaaaga gagccagcaa ctggatgcgt atttgcaggc cttacgtaat cagttgaaca
     3721 gccaacgtca gcttgaggcg gagcgggcgc tggaaagtac cgaattacta gcagaaaaca
     3781 gtgccgattt gccgaaagat atcgtcgcgc aattcaaaat taaccgcgaa ctatcggcgg
     3841 ctttgaatca acaggcgcag cggatggatc tcgttgcctc gcaacagcgt caggctgcca
     3901 gccagacgtt acaggtccgg caggcgttga atacgctgcg tgaacagtcg caatggctgg
     3961 gatcgtccaa tctgctcggt gaagcgttgc gggcgcaggt ggcacggctg ccggaaatgc
     4021 cgaaaccaca acagcttgat accgaaatgg cgcagttgcg tgtgcaacgg ttacgttatg
     4081 aggatctgct taataaacag ccgctgctac ggcaaattca tcaggccgac ggtcagccgc
     4141 tgactgccga gcaaaaccgt attctcgaag cacagctacg cactcagcgt gagttgctga
     4201 actcattgtt gcagggtggc gacacactac tgctggaact gaccaagctg aaagtctcta
     4261 acgggcaact ggaggatgcg ctgaaagagg tgaacgaagc gacgcaccgc tatctgttct
     4321 ggacctctga cgtgcgcccg atgaccatcg cctggccgct ggaaatcgcc caggatctgc
     4381 gtcgtctcat ttcgctggac accttcagtc agttgggcaa agccagtgtg atgatgctga
     4441 ccagcaaaga gacgattttg ccgctgtttg gcgcgttgat tctggtcggt tgcagtattt
     4501 actcgcgccg ctattttacc cgttttctcg aacgttcggc ggcgaaagtc ggcaaagtga
     4561 ctcaggatca cttctggctg acgttacgca ctcttttctg gtcgattctc gtcgcgtcac
     4621 cgctgccggt gctgtggatg acgctgggtt acggcttgcg cgaggcgtgg ccttatccgc
     4681 tggcggtcgc gattggtgat ggcgtcacag ccaccgtgcc gctgctgtgg gtagtgatga
     4741 tttgcgctac ctttgcccgc ccgaacggct tgtttatcgc tcattttggt tggccgcgcg
     4801 aacgtgtttc ccgtgggatg cgctactacc tgatgagcat cgggcttatt gtgccactga
     4861 ttatggcgct gatgatgttc gataatctcg acgaccgcga attttccggt tcgctgggac
     4921 ggctttgctt tatcctcatt tgcggtgcgc tggcggtggt caccctcagc ctgaaaaagg
     4981 ccgggatccc gctgtatctc aacaaagaag gcagcggcga caatattacc aaccatatgt
     5041 tgtggaacat gatgattggc gcgccactgg ttgccattct ggcgtcggcg gtgggttatc
     5101 tggcaacggc acaggcgctg ttagcgaggc ttgaaacctc ggttgccatc tggttcctgc
     5161 tactggtggt ttatcacgtt atccgccgct ggatgctgat ccaacgacgc aggctggcgt
     5221 ttgaccgggc gaagcatcgc cgggcagaga tgttagcgca acgtgcgcgt ggcgaagagg
     5281 aagcacatca tcacagtagc ccggaagggg caattgaagt cgatgaaagc gaagtcgatc
     5341 tcgatgccat cagtgcgcag tccttgcggc tggtgcgctc aattttgatg ttgatcgccc
     5401 tgctttcggt cattgtgctg tggtcagaaa tccattccgc tttcggcttc ctcgaaaata
     5461 tttcgctgtg ggatgtcacc tccacggtac agggcgtaga aagtctggag ccgattaccc
     5521 tcggtgcggt gctgattgcc attctggtgt ttatcatcac cacgcagctg gtgcgcaatc
     5581 tgcccgcgct gctggaactg gcgattttgc agcacctgga tttaacgccg ggtaccggct
     5641 acgccatcac caccatcacc aaatatctgc tgatgttgat tggcgggctg gtcggcttct
     5701 cgatgattgg tattgagtgg tcgaaattgc agtggctggt cgccgcgctc ggtgttggtc
     5761 tcggttttgg tttgcaggaa attttcgcca actttatctc tggcctgatc atcctgttcg
     5821 aaaaaccgat tcgcattggc gatacggtaa cgatccgcga tcttactggt agcgtgacaa
     5881 aaattaacac ccgcgccacc accatcagcg actgggaccg taaagagata atcgtgccga
     5941 acaaggcgtt tattaccgag cagtttatca actggtcgct ctctgactcg gtcacgcgcg
     6001 tggtgttgac gattccggcc cctgccgatg ccaacagtga agaagtgacg gaaatcctgc
     6061 tcaccgcagc gcgtcgctgc tcgctggtaa tcgacaaccc ggcaccggaa gtcttcctgg
     6121 tggatctgca acaggggatt cagattttcg agctgcgtat ttacgccgct gagatgggtc
     6181 accgtatgcc gctacgccat gagatccacc agctgattct ggctggcttc cacgcccacg
     6241 gtatcgatat gccattcccg cccttccaga tgcgtctgga aagcctcaac ggtaaacaaa
     6301 cggggagaac gctgacgtct gcgggtaaag gtcgtcaggc gggaagtttg taatgcatga
     6361 agctgccaaa tgatgtcaac gtggctcatt tagcagctta acatcatctt cagttatcac
     6421 cggccctgga gagtatcatc catacgcatt acttcctgac aactcatcgc cggagagacg
     6481 atgagttgca ggatttcata tgatcagtcg agagctaact caggtttatt cgccatgcga
     6541 ctgcaatagt tctggtaaat cgccatcgct aacagcgaga agaagacagg gccgccaatc
     6601 atccacaatg tgctgtccca gtctccggct tcaaccacag gctgaataat ggtgaagacg
     6661 ttcgcaaatg tcaccaccag aacgacaacc accgtcgcaa tcattgccga catacgcgtt
     6721 ttgaaaatca caaatggtct gtcgagatcc tgacgtgctt taaagaatgg gaaagccagc
     6781 gcgaggaaca ggtaaggaag cgtcatagac acgttcgcca tcagcgtcag cttgttaaag
     6841 aacgccgatg cggtaccgcc accaaacgaa accagcagga tgaagacagt aaccaacccg
     6901 cactgcatcc acatggcgat agacggcatc cccatcgcat tcaggcgcgt catcggttcc
     6961 ggccacaatg ctttcggcgt cccctggatg atggctttca gcggtgaata gcaaagcgta
     7021 aagaacgcac cggtataagc gaggaacatc gaaagtccgg taatacgcgc aaaccatacg
     7081 cccagcgaca atgacgcttc aggagacaaa tgcagtgcgt tacccagcgt caccccgagg
     7141 ctcttcatca gcacataggt aatattgccg aggttaacgg aaccattact taatacctgc
     7201 tgccagtttg tgctgacgcc ccataaaaat attgccagcg aataaccgat tgaaataaca
     7261 atagcggcaa aaacaatacc tttggcaaag ttcttttctg gattttccgt tttatcgacc
     7321 agaccaccaa ccgcttcaat tccgccatag gcaaaaatag caaataccac aaacgatagc
     7381 attgccagac cggactgata acccgggttc ggtgatgcaa ggaaattaat atcctgcgcg
     7441 aaatgcccac catttaataa caaaatagta atgcttacta acagcaatac taaattcaga
     7501 cacattactg caataccgcc caccgcagta atgcgggcaa ttttattaat ccccttagaa
     7561 gcaacgacgg tgaccagaat catccatgcc actgccagca gaccaaccac ctgcgtaggc
     7621 tccagtccgg caatacgcca gtgctgggtc atgtcgctac catagaggaa tgttgagaac
     7681 ggtacccaaa ctttcgcgga ggtactcacc atccagatga tataagagga aaaccacata
     7741 aacgtaccaa tgaaggcaaa acgtggtccg acactattat tcatccagga atagatacca
     7801 ccttcttctt tgcgataagc agctcccatt tcagccatca ttaaggcgaa tggaataaag
     7861 aataacaatg cagaaaatat ataaaaggga atcgcactat aacccattaa gtaataagcc
     7921 gatgggctat tggcaaatcc aaatacggaa gtaaagatca tcaatatgag tcctatcaga
     7981 ctcatctttt ttatcgtgtg aggcatgaaa ccatccttca tttcagcgcg tgtataaaac
     8041 gcgcccactg aactgcactg cccaacattg agcgggcagc gtaaaaacca atacagaatt
     8101 gatacctgga tgatatcaaa tatccagtct taaattgtgg cctttaacag aaaaatgaaa
     8161 acatatgcta cagaatggcg cttatcacgc cattcttcgc tgttaattca gtaatttttc
     8221 agaattatgc ccggtcaacg ctaaaggcga tgacttcagc cagtgtctcc gcgcccagcg
     8281 ccaacatcac cagacgatca acgcctaatg ccacgccgga acagtcaggc ataccgactt
     8341 tcaaggcttc aatcagattc tggtcaatgg ggtgctgcgg caaaccacgc gccgcgcgct
     8401 tacggttatc ttgttcaaag cgttgttgct gctcacgggc atccgtcaat tcatggaaac
     8461 cattcgccag ctcaatacct ttataataag cctcaaagcg ttcagcgacc cgatgatctt
     8521 cggtactgat ttgcgccagt gatgcctggc tggctggaaa gtggtacaca aaggtcggtt
     8581 tttctttacc aatatttggc tctacaccaa aggtaaacag caattgtagc aacgtgtcgc
     8641 ggtcttcttc ggtatctgca acgttgctca aatccagttt cgctgccact tcccgcagtt
     8701 gcgttttgtc ggcagagagc gggtcaattt ccagataacg taagaaagct tgttgataag
     8761 aaaggctttc tgctgccggg cagtccagca cctgttgtaa gagatcgtcc acctcgttca
     8821 tcaaccggta catatcatag tgcggtcgat accactccag catagtgaac tcagggttgt
     8881 gataacgccc catctcttca ttacggaagc tgcggcacag ctggaatacc ggcccacaac
     8941 cagcaaccag caggcgtttc atatggtatt ccgggctggt cattaaccag agattcatcc
     9001 cctgcgaatg cccggggcca acgaaacgtg tctcaaacgg gaccaaatga atatcggtta
     9061 ccgtcgcctg gctcatacaa ggcgtctcca cctccagcac tccacgatcg gcaaagaaac
     9121 gacggatctc cgccataatc gccgcgcgtt ttaataagtt aggaatggat gcgctcggct
     9181 gccaggatgc cgtttcgctc atagttaaat ctccagtttt tgacaagggc acgaagtcta
     9241 ctcgcaacgc gacggcgaga caaattttac gcaggaatca aacagcggtg ggcagtgact
     9301 aaaaaaagca cgatctgatg gtttagtaat taaattaatc atcttcagtg ataatttagc
     9361 cctcttgcgc actaaaaaaa tcgatctcgt caaatttcag acttatccat cagactatac
     9421 tgttgtacct ataaaggagc agtggaatag cgttcgcaga ccgtaacttt caggcactta
     9481 ccctgaagta cggggctgtg ggataaaaac aatctggagg aatgtcgtgc aaacctttca
     9541 agccgatctt gccattgtag gcgccggtgg cgcgggatta cgtgctgcaa ttgctgccgc
     9601 gcaggcaaat ccaaatgcaa aaatcgcact aatctcaaaa gtatacccga tgcgtagcca
     9661 taccgttgct gcagaagggg gctccgccgc tgtcgcgcag gatcatgaca gcttcgaata
     9721 tcactttcac gatacagtag cgggtggcga ctggttgtgt gagcaggatg tcgtggatta
     9781 tttcgtccac cactgcccaa ccgaaatgac ccaactggaa ctgtgggggt gcccatggag
     9841 ccgtcgcccg gatggtagcg tcaacgtacg tcgcttcggc ggcatgaaaa tcgagcgcac
     9901 ctggttcgcc gccgataaga ccggcttcca tatgctgcac acgctgttcc agacctctct
     9961 gcaattcccg cagatccagc gttttgacga acatttcgtg ctggatattc tggttgatga
    10021 tggtcatgtt cgcggcctgg tagcaatgaa catgatggaa ggcacgctgg tgcagatccg
    10081 tgctaacgcg gtcgttatgg ctactggcgg tgcgggtcgc gtttatcgtt acaacaccaa
    10141 cggcggcatc gttaccggtg acggtatggg tatggcgcta agccacggcg ttccgctgcg
    10201 tgacatggaa ttcgttcagt atcacccaac cggtctgcca ggttccggta tcctgatgac
    10261 cgaaggctgc cgcggtgaag gcggtattct ggtcaacaaa aatggctacc gttatctgca
    10321 agattacggc atgggcccgg aaactccgct gggcgagccg aaaaacaaat atatggaact
    10381 gggtccacgc gacaaagttt ctcaggcctt ctggcacgaa tggcgtaaag gcaacaccat
    10441 ctccacgccg cgtggcgatg tggtttatct cgacttgcgt cacctcggcg agaaaaaact
    10501 gcatgaacgt ctgccgttca tctgcgaact ggcgaaagcg tacgttggcg tcgatccggt
    10561 taaagaaccg attccggtac gtccgaccgc acactacacc atgggcggta tcgaaaccga
    10621 tcagaactgt gaaacccgca ttaaaggtct gttcgccgtg ggtgaatgtt cctctgttgg
    10681 tctgcacggt gcaaaccgtc tgggctccaa ctccctggcg gaactggtgg tcttcggccg
    10741 tctggccggt gaacaagcga cagagcgtgc agcaactgcc ggtaatggca acgaagcggc
    10801 aattgaagcg caggcagctg gcgttgaaca acgtctgaaa gatctggtta accaggatgg
    10861 cggcgaaaac tgggcgaaga tccgcgacga aatgggcctg gcaatggaag aaggttgcgg
    10921 tatctaccgt acgccggaac tgatgcagaa aaccatcgac aagctggcag agctgcagga
    10981 acgcttcaag cgcgtgcgca tcaccgacac ctccagcgtg ttcaacaccg acctgctcta
    11041 caccattgaa ctgggccacg gtctgaacgt tgctgaatgt atggcgcact ccgcaatggc
    11101 acgtaaagag tcccgcggcg cacaccagcg tctggacgaa ggttgcaccg agcgtgacga
    11161 cgtcaacttc ctcaaacaca ccctcgcctt ccgcgatgct gatggcacga ctcgcctgga
    11221 gtacagcgac gtgaagatta ctacgctgcc gccagctaaa cgcgtttacg gtggcgaagc
    11281 ggatgcagcc gataaggcgg aagcagccaa taagaaggag aaggcgaatg gctgagatga
    11341 aaaacctgaa aattgaggtg gtgcgctata acccggaagt cgataccgca ccgcatagcg
    11401 cattctatga agtgccttat gacgcaacta cctcattact ggatgcgctg ggctacatca
    11461 aagacaacct ggcaccggac ctgagttacc gctggtcctg ccgtatggcg atttgtggtt
    11521 cctgcggcat gatggttaac aacgtgccaa aactggcatg taaaaccttc ctgcgtgatt
    11581 acaccgacgg tatgaaggtt gaagcgttag ctaacttccc gattgaacgc gatctggtgg
    11641 tcgatatgac ccacttcatc gaaagtctgg aagcgatcaa accgtacatc atcggcaact
    11701 cccgcaccgc ggatcagggt actaacatcc agaccccggc gcagatggca aagtatcacc
    11761 agttctccgg ttgcatcaac tgtggtctgt gctacgccgc gtgcccgcag tttggcctga
    11821 acccagagtt catcggtccg gctgccatta cgctggcgca tcgttataac gaagatagcc
    11881 gcgaccacgg taagaaggag cgtatggcgc agttgaacag ccagaacggc gtatggagct
    11941 gtactttcgt gggctattgc tccgaagtct gcccgaaaca cgtcgatccg gctgcggcca
    12001 ttcagcaggg caaagtagaa agttcgaaag actttcttat cgcgaccctg aaaccacgct
    12061 aaggagtgca acatgacgac taaacgtaaa ccgtatgtac ggccaatgac gtccacctgg
    12121 tggaaaaaat tgccgtttta tcgcttttac atgctgcgcg aaggcacggc ggttccggct
    12181 gtgtggttca gcattgaact gattttcggg ctgtttgccc tgaaaaatgg cccggaagcc
    12241 tgggcgggat tcgtcgactt tttacaaaac ccggttatcg tgatcattaa cctgatcact
    12301 ctggcggcag ctctgctgca caccaaaacc tggtttgaac tggcaccgaa agcggccaat
    12361 atcattgtaa aagacgaaaa aatgggacca gagccaatta tcaaaagtct ctgggcggta
    12421 actgtggttg ccaccatcgt aatcctgttt gttgccctgt actggtaagg agcctgagat
    12481 gattaatcca aatccaaagc gttctgacga accggtattc tggggcctct tcggggccgg
    12541 tggtatgtgg agcgcaatta ttgcgccggt gatgatcctg ctggtgggta ttctgctgcc
    12601 actggggctg tttccgggtg atgcgctgag ctacgagcgc gttctggcgt tcgcgcagag
    12661 cttcattggt cgcgtattcc tgttcctgat gatcgttctg ccgctgtggt gtggtttaca
    12721 ccgtatgcac cacgcgatgc acgatctgaa aattcacgtg cctgcgggca aatgggtttt
    12781 ctacggtctg gctgctatcc tgacagttgt cacgctgatt ggtatcgtta caatctaacg
    12841 tatcgccaat gtaaatccgg cccgcctatg gcgggccgtt ttgtatggaa accagaccct
    12901 atgttcaaaa cgacgctctg cgccttatta attaccgcct cttgctccac atttgctgcc
    12961 cctcaacaaa tcaacgatat tgtgcatcgc acaattaccc cgcttataga gcaacaaaag
    13021 atccccggta tggcggtggc ggtaatttat cagggtaaac cttattactt tacctggggc
    13081 tatgcggaca tcgccaaaaa gcagcccgtc acacagcaaa cgttgtttga gttaggttcg
    13141 gtcagcaaaa catttacggg cgtgcttggt ggcgacgcta ttgctcgagg ggaaatcaag
    13201 ttaagcgatc ccgcaacaaa atactggcct gaacttaccg ctaaacagtg gaacgggatc
    13261 acgctattac atctcgcaac ctacaccgct ggcggcctgc cattgcaggt gccggatgag
    13321 gtgaaatcct caagcgactt gctgcacttc tatcaaaact ggcagcctgt atgggcgcca
    13381 ggaacacaac gtctgtatgc caactccagt atcggtttgt tcggcgcact ggctgtaaag
    13441 ccgtctggtt tgagttttga gcaggcgatg cacactcgtg tcttccagcc actcaaactc
    13501 aaccatacgt ggattaatgt accgcccgca gaagaaaaga attacgcctg gggatatcgc
    13561 gaaggtaagg cagtgcatgt ttcgccaggg gcgttagatg ctgaagctta tggtgtgaag
    13621 tcgaccattg aagatatggc ccgctgggtg caaatcaatt taaaacccct tgatatcaat
    13681 gagaaaacgc ttcaacaagg gatacaagtg gcacaatctc gctactggca aaccggcgat
    13741 atgtatcagg gcctgggctg ggaaatgctg gactggccgg taaatcctga cagcatcatt
    13801 aacggcagtg gcaataaaat tgcactggcg gcacaccccg taaaagcgat tacgccccca
    13861 actcctgcag tacgcgcatc atgggtacat aaaacagggg caaccggcgg atttggtagc
    13921 tatgtcgcgt ttattccaga aaaagagctg ggtatagtga tgctggctaa caaaaactat
    13981 cccaatccag cgagagtcgc cgccgcctgg cagattctca acgctctaca gtaaaatttc
    14041 atcgggtccg aattttcgga ccttttctgc gcttttcctt gctgtcatct acacttagaa
    14101 aaaaaccagt aaggaaacat ttatgcgcct gctccctctc gttgccgcag cgacagctgc
    14161 atttctggtc gttgcctgca gttctcctac gccgccgcgt ggcgtgaccg tagtgaataa
    14221 tttcgacgct aaacgctatc tcggtacctg gtatgagatt gcccgttttg atcaccgctt
    14281 tgaacgtgga ctggaaaaag tcaccgcaac atacagcctg agggatgatg gcggcctgaa
    14341 tgtcattaat aaaggctata accctgacag agaaatgtgg cagcagagtg aagggaaagc
    14401 gtactttacc ggcgcaccaa ctcgcgctgc gctgaaagtg tcattctttg gtcctttcta
    14461 tggcggttat aacgttattg cactcgatcg ggaataccgc catgcgctgg tttgcggacc
    14521 ggaccgcgac tacctgtgga tactctcccg cacgccaacc atttctgacg aagtgaaaca
    14581 ggagatgctg gcagtcgcga cccgggaagg gtttgatgtc agtaagttta tttgggtaca
    14641 gcagcccggt agttagtgag tgctgagttt cagaccaata atccccaata cgattaacgc
    14701 cagactcgcc agacgcatcg ggttagcgga ctcaccgagc agcacaatgc cggtgatggc
    14761 cgcgccgact gcgccaatac ccgtccacac ggcataagcc gtccctaccg gtaacgattt
    14821 catcgcccag gcaagtagcg ccatactgac tatcatcgcc gtcacagtaa taacacttgg
    14881 cgtcaaacga ctaaagccgt gggtatattt caggccaacg gcccatacca cttccagcag
    14941 accagcaata actaagataa tccaggacat atcaggctcc ggagaattgg ggccgtcccc
    15001 ggtgaaaaga ggcgtttgca ggtcgtcctg caaagctgat ggttgaacgg atattttgcc
    15061 aggtggcagg ggaatttcaa ctattacctt tgtgactatc gcaaaaaaac aaacggcacg
    15121 acataactgt cgtaccgtta ttgcttattg ctgcgctttc gttgctgcac cagaaatcgc
    15181 gttaccgcca tcagaaatgt cttcaccaac gccacgcgtg gtgttgcagg cagttaatac
    15241 tgttgaaagc accagaacag aaaagatcgc tgcaattgtc ttcttcacca taacgtcttc
    15301 cttttaggta atgttattta tgtttgccta tagcaaatta ataatagaca acatcgccaa
    15361 aaatgatgga aatctgatga tttttcggaa gagaagaaaa attagctggc agcgcgggag
    15421 atggagttgc cgagatgttt gatgtcttcg ccgaaaccgc gagcggtgtt acagcccgtg
    15481 agcagcgtgc tggcaagcaa aacaagaacg ataaggcgtt tcatcatctc tgacctgaat
    15541 aagtgatggt gcagcctgca ggccgcacca caaccgcatt acttcacgcg agagacgtat
    15601 tcaccagagc gggtatccac tttgatgact tcgccgattt gtacgaacag cggaacttta
    15661 accacagcgc cagtagacag ggtagccggt ttgccgccag tacctgcggt atcacctttc
    15721 aggcccggat cggtatcaac gatttccagt tcaacgaagt tcggcggagt aacggagatc
    15781 ggctgaccat tccacagagt tacgatacac tctgcctgat ccagcagcca tttcgcgttg
    15841 tcgccaattg cttttgcatc agcagacagc tgctcgaaag tttcgttgtt catgaagtgc
    15901 cagaactcac cgtcgttgta caggtaagtc aggttcatat cgacaacatc agcgccttca
    15961 gcggaatcag tagatttgaa ggttttttct acgcgagtac cggtcagcag acgacgcagt
    16021 ttaacgcgag caaatgcctg gcctttaccc ggttttacga attcactcgc ttcaaccgcg
    16081 taaggttcgc cgtctaacat gattttaaga ccagcacgaa aatcgttgct atagtacgtt
    16141 gccataaggc cctctgaaat ttgttaattg gtagctaagc cacaaaatgg cgcatattgt
    16201 aaccctaaat accccatcca gagaagattg gttaacgcaa cttgccgatg ttgtgaccga
    16261 tcctgatgaa cttctgcgtc ttttgaatat agacgcggac gaaaaactgt tggccggacg
    16321 cagcgccaaa aagctgtttg ccctgcgtgt gccccgctca tttatcgatc gcatggagaa
    16381 aggcaatccg gacgatccgc ttttgcgtca ggtaattacc tcgcaagatg agtttgtcgt
    16441 cgcgcccgga ttctccaccg acccactgga agaacagcac agcgtagtgc ctggtttgtt
    16501 gcataaatac cacaaccggg cgcttttgct ggtcaaaggc ggctgcgcgg taaattgccg
    16561 ctattgcttc cgtcgccact tcccctatgc cgaaaatcag ggcaacaagc gtaactggca
    16621 gactgcactt gagtatgttg ctgcgcatcc ggaactggac gagatgattt tctccggcgg
    16681 cgatccgctg atggcgaaag atcacgagct ggactggttg ctcacacaac tggaagccat
    16741 cccgcatatc aaacgtctgc ggattcacag tcgtctgccg attgtgatcc cggcacgcat
    16801 cacagatgcg ctggttgaac gcttttcaca ttctacgctg caaatcttgc tggtgaatca
    16861 catcaaccat gccaatgaga tagatgaaac attccgtcag gcgatggcta agttgcgccg
    16921 ggcgggcgtt actttgctga accagagcgt tctgttacgt ggtgtgaacg ataacgcaca
    16981 aacgctggca aacctgagta atgcgttgtt cgatgcaggc gtgatgccgt attacctgca
    17041 tgtgctcgat aaagtacagg gcgcggcgca ttttatggtg agtgatgacg aagcacggca
    17101 gattatgcgt gagttgctga cactggtgtc gggatatctg gtgccgaaac tggcgcgaga
    17161 aattggcggc gaacccagca aaacgccgct ggatctccag ctacgccagc agtaactgat
    17221 taaacaggga agtcaaacat taaaattgcc tgatgcgcta cgcttatcag gcctacatcg
    17281 tgcattgaaa ttgaatggat ttgcaatgaa ctgtaggccg gataaggcgt ttacgccgca
    17341 tccggcaaga gcaacatacg caagtacagc aacctgtcat taaactggga aatttaatct
    17401 ttgcccgttg cataaccttt aattattcat atacattgct gttgttactc tttatatagc
    17461 taacaccaat aattaacctt tcttttctgt ttatatagag tgagcacggc tacttatgtt
    17521 attttaacaa ctattattac caagcccat
//
LOCUS       DAPGOF010000446         6528 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.guided.234, whole genome shotgun sequence.
ACCESSION   DAPGOF010000446 DAPGOF010000000
VERSION     DAPGOF010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.guided.234"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            40..939
                     /gene="aac(3)-VIa"
                     /locus_tag="RHV13_004894"
     CDS             40..939
                     /gene="aac(3)-VIa"
                     /locus_tag="RHV13_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(3)-VIa"
                     /protein_id="HDV1244307.1"
                     /translation="MTDPRKNGDLHEPATAPATPWSKSELVRQLRDLGVRSGDMVMPH
                     VSLRAVGPLADGPQTLVDALIEAVGPTGNILAFVSWRDSPYEQTLGHDAPPAAIAQSW
                     PAFDPDHAPAYPGFGAINEFIRTYPGCRRSAHPDASMAAIGPDAAWLVAPHEMGAAYG
                     PRSPIARFLAHAGKILSIGAGPDAVTALHYAEAVARIEGKRRVTYSMPLLREGKRVWV
                     TTSDWDSNGILDEYAAPDGPDAVERIARDYLARTRVAQGPVGGAQSRLIDAADIVSFG
                     IEWLEARHAAPAAAALKPKQRRD"
     gene            1146..1466
                     /gene="groES"
                     /locus_tag="RHV13_004895"
     CDS             1146..1466
                     /gene="groES"
                     /locus_tag="RHV13_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDV1244308.1"
                     /translation="MGSPTRKICAAINIKPLYDRVVIKRTEEEKVSAGGIVIPDSATE
                     KPIKGQVVAVGEGKVFDNGNVRAPKVKVGDQVLFGKYAGTEVKLDGTEYLVVKEDDIF
                     AVLG"
     gene            1522..3159
                     /gene="groL"
                     /locus_tag="RHV13_004896"
     CDS             1522..3159
                     /gene="groL"
                     /locus_tag="RHV13_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005411226.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDV1244309.1"
                     /translation="MAAKDIRFGEDARARMVRGVNVLANAVKATLGPKGRNVVLEKSF
                     GAPTITKDGVSVAKEIELADKFENMGAQMVKEVASKTSDNAGDGTTTATVLAQALIRE
                     GMKAVAAGMNPMDLKRGIDKAVTSAVEELKKISKPCSTSKEIAQVGSISANSDTDIGE
                     LIAKAMDKVGKEGVITVEEGSGLENELDVVEGMQFDRGYLSPYFINNPQSMQAELEDP
                     FILLHDKKISNVRDLLPILEGVAKAGKPLLIVAEDVEGEALATLVVNTIRGIVKVCAV
                     KAPGFGDRRKAMLEDMAILTGGTVISEEVGLSLEKATINDLGRAKKVQVSKENTTIID
                     GAGDTADIEARIKQIKAQIEETTSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGIVPGGGVALIRAKAAIAELKGANEDQNHGIAIALRAMEAP
                     LREIVTNAGDEPSVVLNRVAEGTGAFGYNAANGEFGDMIEFGILDPTKVTRSALQNAA
                     SIAGLMITTEAMVAEAPKKEEPAAPGGGMGGMGGMDF"
     gene            3348..4877
                     /locus_tag="RHV13_004897"
     CDS             3348..4877
                     /locus_tag="RHV13_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV1244310.1"
                     /translation="MPRLTAPRRQAEVCAPLPAAHAAARYARHLPERTLLYALVQAHY
                     PDFIARLEAEDRPLPEYVREEFETYLRCGVLEHGFLRVVCEQCRAERLVAFSCKKRGF
                     CPSCGARRMAESARHLVEEVFGPRPVRQWVLSFPYPLRFLFASKPEAIGPVLGIVHRV
                     IAGWLADQAGVDHASAQCGAVTLIQRFGSALNLNVHFHMLWLDGVYEDTTERPQRKPR
                     LHRTRAPTSAQLTELAGTIAHRVCRHLSRRGWLEGEDESVFLSDSAGSDDGMDGLRMS
                     SMTYRIATGRDAGRKVVTLQILPGDAGSLEGDAGKVGGFSLHAGVAAEAHESHKLEKL
                     CRYITRPAISEQRLSISPQGRVRYQLKTPWRNGTTHVEWDAVDFIAKLAALVPPPRAH
                     LTRFHGVFAPNANLRAQLTPSGRGRRPAGDAVPVDASAHDEPRSPEQKRRAMSWAQRL
                     KRVFSIDVTTCAHCGGAVRIVASIEDPKAIRAILAHFEKHGALEQAHYRPAARAPPPA
                     A"
     gene            5145..6380
                     /locus_tag="RHV13_004898"
     CDS             5145..6380
                     /locus_tag="RHV13_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEc58 family transposase"
                     /protein_id="HDV1244311.1"
                     /translation="MPRKKKTPVVTPAIDLPKEFLEKLIPGPMDAAGVEAVFQQLKKA
                     VIERALGAELGLHLADAEGGSGNHRNGRSGKTVLTDEGPLRIDVPRDRTGTFEPQLIP
                     KHERRFAGFDDRIVSMYARGMTVREIQGHLAEMYSVEVSPEFISKVTDEVMAEVTAWQ
                     ARPLEAMYPVVFFDALRVKIREDGVVRNKAVYLALGVLADGTRDILGLWIENTEGAKF
                     WMKVFNDLKTRGVNDILIGVADGLKGFPEALEAVFPATTLQTCIVHLIRNSLDYAGWK
                     DRKALAAAIKPIYTAPTAEAAEAELDAFEAGDWGERFPTVVAAWRRAWTRVVPFFAFP
                     PDVRRVIYTTNAIESVHARLRKIIKTRGHFPSDEAATKLIWLALRNITAEWSRASQHW
                     KAAMNQFAIIYEDRFTLAR"
BASE COUNT         1224 a         2173 c         2172 g          959 t
ORIGIN      
        1 tgcataataa gccctacaca aattgggaga tatatcatga tgactgatcc ccgcaaaaac
       61 ggcgatttgc acgaacccgc gacggcaccc gcgacgccct ggtccaaaag cgagctggtc
      121 cggcaattgc gcgacctcgg cgtgcgctca ggcgatatgg tgatgccgca tgtgtcgttg
      181 cgcgccgtcg ggccgctggc ggacggaccg cagacacttg tcgatgcgct gatcgaggcc
      241 gtcggcccca ccgggaatat tctcgccttc gtctcgtggc gcgattcgcc ctatgaacag
      301 acgctgggtc atgatgcgcc gcccgccgcc atcgcccaaa gctggcctgc gttcgacccc
      361 gaccatgcgc ccgcctaccc cggctttggc gcgatcaacg aatttatccg aacctatccg
      421 gggtgtcggc gcagcgccca tcccgacgca tcgatggcgg cgatcgggcc cgatgcggcg
      481 tggctggtgg cgccgcacga gatgggcgcc gcttatggcc cccgctcgcc gatcgcgcgt
      541 tttctcgccc acgcaggaaa aatcctgtcg atcggcgccg ggcccgatgc agtcaccgcg
      601 ctccattatg ccgaagcggt ggcgcggatc gagggcaagc gccgcgtcac ttattcgatg
      661 cccttactgc gcgaaggcaa gcgcgtctgg gtcaccacgt ccgactggga ttcgaacggc
      721 atcctcgacg aatatgccgc gcccgacggc cccgacgcgg tcgaacggat cgcccgcgac
      781 tatctcgccc gcaccagggt tgcgcaaggc ccggtcggcg gcgcgcaatc ccggctgatc
      841 gacgcggccg atatcgtttc cttcggcatc gaatggctcg aggcgcgcca cgccgcgcca
      901 gcggcggcag cgctgaagcc gaaacaacgc cgcgactgag tcgtcgatta ttccggacat
      961 atattcctcc ctctcgccca gccgcatagc atcaggccca caagcccgcc gcctcttcaa
     1021 caaggatgtc gggacgttct tcagtaaaga acagcttggc accctcgaca aggatgattg
     1081 cccgaggcga tcaggcggcg aatttttgcg cgcgcgcacg gtcgcggcgg cgcgcgataa
     1141 aggcgatggg tagccccacg aggaagatat gcgcggcgat taacatcaag ccgctgtacg
     1201 accgcgtcgt catcaagcgc accgaggaag agaaggtctc cgccggtggt atcgtgatcc
     1261 cggattcggc caccgagaag ccgatcaagg gccaggtcgt cgccgtgggc gagggcaagg
     1321 tgttcgacaa cggcaacgtg cgcgcgccga aggtcaaggt cggcgaccag gtgctgttcg
     1381 gcaagtacgc cggcaccgag gtcaagctcg acggcaccga gtacctggtg gtgaaggaag
     1441 acgacatctt cgccgtgctg ggctgagccc gccgcttcgc cgcatcccaa cccgcaatca
     1501 cagaactgag gtaattccgc aatggctgcc aaggatattc gtttcggtga agacgcccgc
     1561 gcccgcatgg tccgcggcgt gaacgtgctc gccaacgccg tcaaggcgac cctgggcccg
     1621 aagggccgca acgtcgtgct cgagaagagc ttcggcgccc cgacgatcac caaggacggc
     1681 gtgtccgtcg ccaaggagat cgaactggcc gacaagttcg agaacatggg cgcgcagatg
     1741 gtcaaggaag tcgcttccaa gacctccgac aacgccggcg acggcaccac caccgccacc
     1801 gtgctggccc aggccctgat ccgcgagggc atgaaggccg tggccgccgg catgaacccg
     1861 atggacctca agcgcggcat cgacaaggcc gtcacctcgg ccgtcgagga gctgaagaag
     1921 atctccaagc cctgctcgac cagcaaggag atcgcccagg tcggttcgat ctcggccaac
     1981 tccgacaccg acatcggcga gctgatcgcc aaggccatgg acaaggtcgg caaggaaggc
     2041 gtgatcaccg tcgaggaggg ctcgggcctg gagaacgagc tcgacgtcgt cgagggcatg
     2101 cagttcgacc gcggctacct gtcgccgtac ttcatcaaca acccgcagtc gatgcaggcc
     2161 gagctggagg atccgttcat cctgctgcac gacaagaaga tctcgaacgt ccgcgacctg
     2221 ctgccgatcc tcgagggcgt ggccaaggcc ggcaagccgc tgctgatcgt cgccgaggac
     2281 gtcgagggcg aggcgctggc cacgctggtg gtcaacacca tccgcggcat cgtgaaggtc
     2341 tgcgcggtca aggccccggg cttcggcgac cgccgcaagg cgatgctgga ggacatggcc
     2401 atcctcaccg gtggcaccgt gatctccgag gaagtcggcc tctcgctcga gaaggcgacc
     2461 atcaacgacc tcggccgcgc gaagaaggtg caggtctcga aggagaacac caccatcatc
     2521 gacggcgccg gcgacaccgc ggacatcgaa gcccgcatca agcagatcaa ggcgcagatc
     2581 gaggagacca cctcggacta cgaccgcgag aagctgcagg agcgcgtggc caagctggcc
     2641 gggggcgttg cggtgatcaa ggtcggcgcc gccaccgaag tcgagatgaa ggaaaagaag
     2701 gcgcgcgtcg aagacgccct gcatgccacc cgtgcggcgg tcgaggaagg catcgtcccg
     2761 ggcggcggcg tcgccctgat ccgtgccaag gccgcgatcg ccgagctgaa gggcgccaac
     2821 gaggaccaga accacggcat cgcgatcgcc ctgcgcgcga tggaagcccc gctgcgcgag
     2881 atcgtcacca acgccggcga cgagccgagc gtggtgctga accgcgtcgc cgaaggcacc
     2941 ggcgcgttcg gctacaacgc cgccaacggc gagttcggcg acatgatcga gttcggcatc
     3001 ctggacccga ccaaggtcac gcgctccgcg ctgcagaacg cggcgtccat cgccggcctg
     3061 atgatcacca ccgaagcgat ggtggccgaa gcgccgaaga aggaagagcc ggccgctccg
     3121 ggcggcggca tgggcggcat gggcggcatg gatttctaag ccccgcgatc catcaagcaa
     3181 gaccacaaag cccggcctcg tgccgggctt tgtgcgttct ggcgtccgag gcgggagact
     3241 tcctacccgc cccgcggcaa tgtctgacgc gaagatcaga aaacgccgat atgaacgcgt
     3301 gctcgcgggc gcaaccctga gcagccgtcc ctgcaacgga gcgctgcgtg ccgcgcctga
     3361 ccgcaccccg gcggcaggcc gaggtgtgcg cgccactgcc ggccgcccac gccgctgcgc
     3421 gttacgcgcg ccacctgccc gagcgcacgc tgctgtacgc gctagtgcag gcgcactacc
     3481 cggacttcat cgcgcgtctt gaggccgaag accgcccgct gcccgagtat gtgcgcgagg
     3541 agttcgagac ctacctgcgt tgcggcgtgc tcgagcacgg tttcctgcgc gtggtgtgcg
     3601 agcagtgccg ggccgagagg ctggtggcct tctcctgcaa gaagcgcggc ttctgcccca
     3661 gctgcggggc gcggcgcatg gccgagagcg cgcggcacct ggtggaggag gtgtttggcc
     3721 cgcggccggt gcggcaatgg gtgctgagtt ttccgtaccc gctgcgcttc ctgttcgcca
     3781 gcaagccgga ggccatcggc ccggtgctgg gcatcgtgca tcgtgtgatc gccggctggt
     3841 tggccgatca agccggcgtc gaccacgcca gcgcgcaatg cggcgcggtg accctgatcc
     3901 agcgcttcgg cagcgcgctg aacctgaatg ttcacttcca catgctgtgg ctcgacggcg
     3961 tgtacgagga caccaccgag cgtccgcagc gcaagccgcg cctgcaccgc acccgtgcgc
     4021 ccacatcggc gcaactgacg gaactggccg gcaccatcgc gcatcgcgtg tgccggcacc
     4081 tgtcgcgccg cggctggctc gaaggcgaag acgaatccgt gttcctgtcc gacagcgcgg
     4141 gtagcgacga cggcatggat gggctgcgga tgagttcgat gacctaccgc atcgccaccg
     4201 gtcgcgacgc tggccgcaag gtcgtcacgc tgcaaatatt gcccggtgac gcaggctcgc
     4261 tggagggcga cgccggcaag gtcggcggct tctcgctgca tgccggcgtg gccgcggaag
     4321 cacacgaaag ccacaagctc gaaaagctgt gccgctacat cacgcgcccg gcgatcagcg
     4381 agcagcggct gtcgatctca ccgcagggta gggtgcggta tcagctcaag acgccgtggc
     4441 gaaatgggac cacgcatgtc gaatgggatg cggtggactt catcgccaag ctggcggcac
     4501 tggtcccgcc acctcgcgcg catctcaccc gcttccacgg cgtattcgcc ccgaatgcaa
     4561 acctgcgtgc gcagctgacg ccctcggggc gcggcaggcg gcctgcgggc gatgcggtgc
     4621 cggttgacgc aagcgcccac gacgagccgc gcagccccga gcagaagcgc cgtgcgatga
     4681 gctgggcgca acggctcaag cgggtctttt ccatcgacgt caccacctgt gcccactgcg
     4741 gcggcgcggt gcggatcgtc gccagcatcg aagaccccaa ggccattcgc gccatcctcg
     4801 cccacttcga gaaacacggc gcgctggagc aagcgcacta ccggcccgca gcgcgcgcgc
     4861 cgccgccggc cgcgtgacga ggtgccggcc acacagccgg cagccaagcc agagtccgat
     4921 ccgatgcggc cacgaccccg cagggctgcg ctcggccctg tgccgggttt cggtgagaaa
     4981 tggctacgca ctgagccgct gcgtggcccc gcgatgtcga aaacccacgc atgaaccccc
     5041 gatctgtgcc cgatctgtgc ccaaagcggc gcttgcgcgg ccgcttccga gggtgtcaga
     5101 aattttgtgt gagaggcata ccatgatgct gccaaggagc atgtatgcca cgcaagaaga
     5161 agacgccggt ggtcacgccg gccattgatc tgccgaagga atttctcgag aagctgatcc
     5221 cgggtccgat ggacgcggcc ggggtcgaag ccgtgttcca gcagctgaag aaggccgtga
     5281 tcgagcgggc gctgggcgcg gagctcggcc tgcacctggc cgacgccgag ggcgggagcg
     5341 gcaaccaccg caacggccgc agcggcaaga cggtgctgac cgacgagggt ccgctgcgca
     5401 tcgacgtgcc gcgcgaccgg acgggtacgt tcgagccgca gctgatcccc aagcacgagc
     5461 gtcgcttcgc cgggttcgac gatcggatcg tctcgatgta cgcccgcggc atgacggtgc
     5521 gtgagatcca ggggcacctg gccgagatgt acagcgtcga ggtgtcgccg gagttcatca
     5581 gcaaggtcac cgacgaggtg atggccgagg tcaccgcatg gcaggcgcgg ccgctggaag
     5641 cgatgtatcc ggtggtgttc ttcgacgcgc tgcgggtgaa gatccgcgag gacggcgtgg
     5701 tgcgcaacaa ggcggtgtat ctggcgctgg gcgtgctggc cgacggcacc cgcgacatcc
     5761 tcgggctgtg gatcgagaac accgagggcg cgaagttctg gatgaaggtg ttcaacgacc
     5821 tcaagacgcg cggcgtcaac gacatcctga tcggcgtggc tgacggcctg aagggcttcc
     5881 cggaggcgct ggaagcggtg ttcccggcga ccacgctgca gacctgcatc gtgcacctga
     5941 tccgcaacag cctggactat gcgggctgga aggaccgcaa ggcgctggcg gcggcgatca
     6001 agccgatcta cacggccccg accgccgagg ccgcagaggc cgagctggat gcgttcgagg
     6061 ccggcgactg gggcgagcgc ttcccgacgg tggtcgccgc ctggcgccgt gcctggacgc
     6121 gcgtggtgcc gttcttcgcg ttcccgccgg acgtgcgccg ggtgatctac acgaccaacg
     6181 caatcgagag cgtgcatgcg cgcctgcgca agatcatcaa gacccgcggc cacttcccca
     6241 gcgacgaggc cgccaccaag ctgatctggc tggcgctgcg gaacatcacc gccgagtgga
     6301 gccgcgcgtc tcagcactgg aaggcggcga tgaaccagtt cgcgataatc tacgaagacc
     6361 gattcacgct ggctcgctga aagagacttc cggactaaac cgcctcacac acaaaaaatc
     6421 ggacacgccc ccgcttccta cctgccagac tcgcaaaaaa gggcggttga acttcctata
     6481 cccacaaagt tagatgcact aagcacataa ttgctcacag ccaaacta
//
LOCUS       DAPGOF010000447         6076 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.guided.240, whole genome shotgun sequence.
ACCESSION   DAPGOF010000447 DAPGOF010000000
VERSION     DAPGOF010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.guided.240"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            133..336
                     /locus_tag="RHV13_004899"
                     /pseudo
     CDS             133..336
                     /locus_tag="RHV13_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="mutations in this gene result in a compromised
                     ability for drug-inducible mexXY expression; expression is
                     inducible by the same ribosome-targeting agents that
                     induce mexXY; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            335..856
                     /locus_tag="RHV13_004900"
                     /pseudo
     CDS             335..856
                     /locus_tag="RHV13_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(913..2370)
                     /gene="pepD"
                     /locus_tag="RHV13_004901"
     CDS             complement(913..2370)
                     /gene="pepD"
                     /locus_tag="RHV13_004901"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HDV1244312.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVIHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREEVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQDILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2631..3089
                     /gene="gpt"
                     /locus_tag="RHV13_004902"
     CDS             2631..3089
                     /gene="gpt"
                     /locus_tag="RHV13_004902"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDV1244313.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3181..4425
                     /gene="frsA"
                     /locus_tag="RHV13_004903"
     CDS             3181..4425
                     /gene="frsA"
                     /locus_tag="RHV13_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HDV1244314.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4483..4884
                     /gene="crl"
                     /locus_tag="RHV13_004904"
     CDS             4483..4884
                     /gene="crl"
                     /locus_tag="RHV13_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HDV1244315.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(4923..5978)
                     /gene="phoE"
                     /locus_tag="RHV13_004905"
     CDS             complement(4923..5978)
                     /gene="phoE"
                     /locus_tag="RHV13_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HDV1244316.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPISGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
BASE COUNT         1494 a         1485 c         1538 g         1559 t
ORIGIN      
        1 aaataattcg agttgcagga aggcgacaag cgagtgaatc gccaggagct tacataagta
       61 agtgactggg gtgaacgaac gcagtcgcag taaatgcaac ttgaagtatg acgagtataa
      121 atggaatggt acatgggcaa atatattctt cccttatccg atgcggtatt taccatcgca
      181 tctgatgacc tgtggatcga gagtttagcg atccaacaat tacacaccac ggcaaattta
      241 cccaacatgc agcgcgtagt ggggatgcca gatttacacc ccggacgcgg ctacccgatt
      301 ggcgcagcgt tcttctctgt tggtcgtttt tacccgacaa gacgtcgcgg taacggtgct
      361 ggaaacagaa acgggctgct actctgacac gctgcgttcg gcgctggttt ctctggatgg
      421 cgataacgca tgggcattaa gcgaaagctg gtgtggcact attcagtgga tttgtccgag
      481 tccgtatcgg cctcatcatg ggcgcaaaaa ctggtttctg ggcattgggc gttttaccgc
      541 tgatgagcag gaacaatcgg atgcaatccg ttatgagacg ttgcgttcgt cggggccggg
      601 cggtcaacat gtcaataaaa ccgactcggc ggtacgcgcc acgcatttgg catccggtat
      661 tagcgtgaag gttcagtcag agcgtagtca gcatgctaac aagcggctgg cacgattgct
      721 gattgcctgg aggctggagc agcagcaaca ggaaaatagc gcggtgctga aatcgcagcg
      781 gcgaatgttc catcaccaga ttgaacgtgg caacccgaga cggacattta cagggatggc
      841 ttttatcgaa ggataatgaa ggatgaaact gccggaaggc gattaaacgc catccggcag
      901 caaatcaaat aattacttcg ccggaatttc tttcagcagt tcagtcagca gcgtccagta
      961 atgacctacg ctttcgatgt gaacttgctc atccggagag tgtggaccgg tgatagttgg
     1021 cccgatagaa accatgtcca tttccggata cggttttttg aacagaccac attccaggcc
     1081 cgcgtggata atctggatgt tcggtgtttt gttgaacaga cgctgatagg tttcacgtac
     1141 cagatgcatc accggagaat tagcgtccgg ctgccagcca ggatatgcgc ctttcgcttc
     1201 ggttttcgcg ccagctagtt tacccagcga atccagcatg ctcaccacgt agtctttacc
     1261 gctgtcgatc agtgaacgga tcaggcagtg aatttctacg ttattgtcag tcatggtcac
     1321 cacaccgacg ttcagggagg tttcaaccac accttttgcc acgtcggaat tgcggatcac
     1381 accgttcgga gtggcgttca gcagacgaat aaaggtatcg cgagatttcg caatcagggc
     1441 agctttatcg ttcgctacag agtccagcaa caaggccaga tttttctctt tttctgccag
     1501 ctcgtttttc agaatgtcct gataggtatt caccagagat ttcagtgcgt cgactttatc
     1561 agctgcgaca gcaatggtcg caaaggcttc acgcgggatg gcgttacgca gtgtaccgcc
     1621 gttgaaatcg ataaggcgca gatccagttc ttccgcatga cccgccaaga agcgcaccag
     1681 cagtttgttg gcattaccga ggccaacgtg gatttcaccg ccggagtgac cgcctttcag
     1741 accttttaag gttaacttga aggtttcaaa accagctgga acctcttcac gatctaaatg
     1801 caggttggag gtgaagtcga taccccccgc gcaacccatg tagatttcac cttcttcttc
     1861 ggagtcggtg ttaatcagaa tatcagcctg caaccagttg ctctgtaagc cgaacgcacc
     1921 gtccataccg gcttcttcgg tcatggtcag cagcacttcc agcgggccgt gaatcacgtt
     1981 ttcgtcagcc agaaccgcca gcgcagaggc cataccaatg ccgttatccg cacccagcgt
     2041 ggtgccgcgc gctttaaccc attcgccatc aatataaggc tggataggat ctttcgtgaa
     2101 gtcatgcaca gtgtcgttat ttttctgcgg caccatatcg aggtgggcct gcaagacgac
     2161 cggtttacga ttttccatac ctgcggtagc aggtttacga atcaggatat tacctacctg
     2221 atcgcgttcg acatggaaac ctttctcttt tgcccaacca acaatgtatt cagcgagttg
     2281 ctcttcatga taggacgggt gaggaataga acagattttg gcaaaaatat cccacagcgg
     2341 ctgtggagat aattgagaca gttcagacac gttaagtctc cttgtcgatc acccgcaaaa
     2401 cagtattgca ggtcacaggg ttagcagaaa atgttgtcaa cacaagacat gcttgcgaga
     2461 tatgtttgag aataccactt tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg
     2521 actcatgtga aatactggtt tttagtgcgc cagatctcta taatctcgcg caacctattt
     2581 tcccctcgaa cactttttaa gccgtagata aacaggctgg gacacttcac atgagcgaaa
     2641 aatacatcgt cacctgggac atgttgcaga tccatgcacg taaactcgca agccgactga
     2701 tgccttctga acaatggaaa ggcattattg ccgtaagccg tggcggtctg gtaccgggtg
     2761 cgttactggc gcgtgaactg ggtattcgtc atgtcgatac cgtttgtatt tccagctacg
     2821 atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc agaaggcgat ggcgaaggct
     2881 tcatcgttat tgatgacctg gtggataccg gtggtactgc ggttgcgatt cgtgaaatgt
     2941 atccaaaagc gcactttgtc accatctttg caaaaccggc tggtcgtccg ctggttgatg
     3001 attatgttgt tgatatcccg caagatacct ggatcgaaca gccgtgggat atgggcgtcg
     3061 tattcgtccc gccaatctcc ggtcgctaat cttttcaacg cctggcactg ccgggcgttg
     3121 ttctttttaa cttcaggcgg gttacaatag tttccagtaa gtattctgga ggctgcatcc
     3181 atgacacagg caaacctgag cgaaaccctg ttcaaacccc gctttaaaca tcctgaaacc
     3241 tcgacgctag tccgccgctt taatcacggc gcacaaccgc ctgtgcagtc ggcccttgat
     3301 ggtaaaacca tccctcactg gtatcgcatg attaaccgtc tgatgtggat ctggcgcggc
     3361 attgacccac gtgaaatcct cgacgtccag gcacgtattg tgatgagcga tgctgaacgt
     3421 accgatgatg atttatacga taccgtgatt ggctaccgtg gtggcaactg gatttatgag
     3481 tgggccaccc aggcgatggt gtggcaacaa aaagcctgtg cggaagaaga tccgcaactc
     3541 agtggtcgac actggctgca tgcggctacg ttgtacaaca ttgccgccta tcctcatctg
     3601 aaaggagatg acctggccga gcaagcgcag gctttatcaa accgcgccta tgaagaggcc
     3661 gcccagcgtc tgccgggcac gatgcggcag atggagttta ccgtacccgg cggtgcgccc
     3721 atcaccggct ttttgcatat gccgaaaggc gatggcccgt tcccgacagt attaatgtgt
     3781 ggtggtctgg atgcgatgca gacggactat tacagcctgt atgaacgtta ttttgcgccg
     3841 cgcggcattg cgatgctgac tattgatatg ccgtcggtgg gcttttcttc aaaatggaag
     3901 ctcacccagg actccagcct gttgcatcag cacgtcttaa aggcgctgcc taacgtaccg
     3961 tgggtggatc acactcgcgt cgcggccttt ggtttccgtt tcggcgctaa cgttgccgtg
     4021 cgtctggcat accttgaatc gccgcgtctg aaagcggttg cctgtcttgg tccggtagtt
     4081 cataccctgt tgagtgattt taagtgccag caacaggtgc cggaaatgta tcttgacgtt
     4141 ctggcgagtc gtttggggat gcatgatgct tccgatgacg cgttgcgcgt ggagctgaat
     4201 cgctattcat taaaagtgca aggattgctg ggacgtcgct gcccaacgcc aatgttatca
     4261 ggctactgga agaacgatcc gttcagcccg gaagaggact cacgcttaat cacctcatca
     4321 tctgctgacg gtaaattatt agagatccca tttaacccgg tgtatcggaa ttttgacaaa
     4381 ggtcttcagg aaatcaccgg ctggatcgaa aaacgcttgt gttaaaaatt tgctaaattt
     4441 tgccaatttg gtaaaacagt tgcatcacaa caggagatag caatgacgtt accgagtgga
     4501 cacccgaaga gcagattgat caaaaaattt accgcactag gcccgtatat tcgtgaaggt
     4561 aagtgcgaag ataatcgatt ctttttcgat tgtctggctg tatgcgtcaa cgtgaaaccg
     4621 gcaccggaag tgcgtgaatt ctggggctgg tggatggagc tggaagcgca ggaatcccgt
     4681 tttacctaca gctaccagtt tggtctgttc gataaagcag gcgactggaa gagtgttccg
     4741 gtaaaagata ctgaagtggt tgaacgactg gagcacaccc tgcgtgagtt tcacgagaag
     4801 ctgcgtgaac tgctgacgac gctgaatctg aagctggaac cggcggatga ttttcgtgac
     4861 gagccggtga agttaacggc gtgagtgaaa tgtgccggat gcatcacatc cggcaataat
     4921 cattaaaact gataggtcat gccaaccgcg acaatatcat cattattaat attcaatttg
     4981 ttatcgctat ccagttggtt gattttataa tcaacaaacg ctgacatatt tttgttgaaa
     5041 taatatgtag ccccgacatc gatataattg accagatctt catcaccgat accttcaata
     5101 tctttccctt tcgataagac ataacccagc gatggacgca gaccaaagtc aaactggtat
     5161 tgagcgaccg cttcaaagtt ctgagtttta ttggcaaagc cgccagatat tggcgtcatt
     5221 ttgcgtgttt cagaataaaa agttgccaga taaatattat tggcatcgta tttcagacct
     5281 gttgcccatg cttctgcacg cttgcctgtg ccacggcttt gcaggttctg ctcgttggtg
     5341 cgatctgagt tggtataggc cccactaatg gcgaaatcgc tgccgccaaa gtcatatgtc
     5401 aatgacgtgc cgaagccatc gccgttttgc tttttaacgt cgcggttttc gtttttccct
     5461 tgatattgca gggttaagtt caggccatcg ataacgccga agaagtcggt gttccgatac
     5521 gtcgccagac cgctggcgcg tttggtcata aagttgtcgg tctgcgccga ggagtcgcca
     5581 ccaaattccg ggaacatatc ggtccaggct tccacgtcat acaacgcgcc caggttacga
     5641 ccataatcga aagaacccaa atctttatat ttcaacccgg caaaagcgag acgcgttttt
     5701 tgctgtgcag tatcactctc tgctttatta ccggcaaact ctgcttccca acgaccataa
     5761 ccagtcagtt gatcgttaat ttgtgtttcg cctttaaaac caaaacggat ataactctgg
     5821 tcgccatctt tactgtcgtt atcactcata taatgcatgg ctttaacttt gccatagaca
     5881 tccagtttat taccgtcttt gttatatatt tctgcggcct gtacggatgc agatgccaca
     5941 atgcccatca ccactaatgc cagagtgctc tttttcattt tcattcctga ttttaattaa
     6001 cgcgcgaata ttcagcggga gagccccgtt gaaaacagga aagtttttaa cctgagattg
     6061 ttaaagatat attaca
//
LOCUS       DAPGOF010000448         5222 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.guided.255, whole genome shotgun sequence.
ACCESSION   DAPGOF010000448 DAPGOF010000000
VERSION     DAPGOF010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.guided.255"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            138..470
                     /gene="qacG2"
                     /locus_tag="RHV13_004906"
     CDS             138..470
                     /gene="qacG2"
                     /locus_tag="RHV13_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacG2"
                     /protein_id="HDV1244317.1"
                     /translation="MKNWLFLATAIIFEVIATSALKSSEGFTRLVPSFIVVAGYAAAF
                     YFLSLTLKSIPVGIAYAVWSGLGIVLVTAIAWVLHGQKLDMWGFVGVGFIISGVAVLN
                     LLSKASVH"
     gene            complement(675..1298)
                     /locus_tag="RHV13_004907"
     CDS             complement(675..1298)
                     /locus_tag="RHV13_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003155741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDV1244318.1"
                     /translation="MLIGYMRVSKADGSQSTNLQRDALIAAGVSLAHLYEDLASGRRD
                     DRPGLAACLKALREGDTLIVWKLDRLGRDLRHLINTVHDLTARSVGLKVLTGHGAAVD
                     TTTAAGKLVFGIFAALAEFERELISERTVAGLISARARGRKGGRPFKMTAAKLRLAMA
                     SMGQPETKVGDLCEELGITRQTLYRHVSPKGELRPDGVKLLSLGSAA"
     gene            complement(1360..2577)
                     /locus_tag="RHV13_004908"
     CDS             complement(1360..2577)
                     /locus_tag="RHV13_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HDV1244319.1"
                     /translation="MKPAPRWPLHPAPKEGEALSSWLNRVALCYHMEEPDLLEHDLGH
                     GQVDDLDTAPPLSLLALLSQRSGIELDRLRCMSFAGWVPWLLDSLDDQIPDALETYAF
                     QLSVLLPRLRRKTRSITSWRAWLPSQPINRACPLCLSDPENQAVLLAWKLPLMLSCPL
                     HGCWLESYWGVPGRFLGWENADAEPRTASDAIAAMDQRTWQALTTGHVELPRRRIHAG
                     LWFRLLRTLLDELNTPLSACGTCAGYPRQVWEGCGHPLRAGQSLWRPYETLNPIVRLQ
                     MLEAAATAISLIEVRDISPPGEQAKLFWSEPQTGFTSGLPTKAPKPEPINHWQRAVQA
                     IDEAIIEARHNPETARSLFALASYGRRDPASLERLRATFVKEGIPPEFLSHYLPDAPF
                     ACLKQNDGLSDKF"
     gene            complement(2574..3482)
                     /locus_tag="RHV13_004909"
     CDS             complement(2574..3482)
                     /locus_tag="RHV13_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003155746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniB family NTP-binding protein"
                     /protein_id="HDV1244320.1"
                     /translation="MDEYPIIDLSHLLPAAQGLARLPADERIQRLRADRWIGYPRAVE
                     ALNRLEALYAWPNKQRMPNLLLVGPTNNGKSMIVEKFRRTHPASSDADQEHIPVLVVQ
                     MPSEPSVIRFYVALLAAMGAPLRPRPRLPEMEQLALALLRKVGVRMLVIDELHNVLAG
                     NSVNRREFLNLLRFLGNELRIPLVGVGTRDAYLAIRSDDQLENRFEPMMLPVWEANDD
                     CCSLLASFAASLPLRRPSPIATLDMARYLLTRSEGTIGELAHLLMAAAIVAVESGEEA
                     INHRTLSMAVYTGPSERRRQFERELM"
     gene            complement(3485..5164)
                     /locus_tag="RHV13_004910"
     CDS             complement(3485..5164)
                     /locus_tag="RHV13_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HDV1244321.1"
                     /translation="MATDTPRIPEQGVATLPDEAWERARRRAEIISPLAQSETVGHEA
                     ADMAAQALGLSRRQVYVLIRRARQGSGLVTDLVPGQSGGGKGKGRLPEPVERVIHELL
                     QKRFLTKQKRSLAAFHREVTQVCKAQKLRVPARNTVALRIASLDPRKVIRRREGQDAA
                     RDLQGVGGEPPAVTAPLEQVQIDHTVIDLIVVDDRDRQPIGRPYLTLAIDVFTRCVLG
                     MVVTLEAPSAVSVGLCLVHVACDKRPWLEGLNVEMDWQMSGKPLLLYLDNAAEFKSEA
                     LRRGCEQHGIRLDYRPLGQPHYGGIVERIIGTAMQMIHDELPGTTFSNPDQRGDYDSE
                     NKAALTLRELERWLTLAVGTYHGSVHNGLLQPPAARWAEAVARVGVPAVVTRATSFLV
                     DFLPILRRTLTRTGFVIDHIHYYADALKPWIARRERWPSFLIRRDPRDISRIWVLEPE
                     GQHYLEIPYRTLSHPAVTLWEQRQALAKLRQQGREQVDESALFRMIGQMREIVTSAQK
                     ATRKARRDADRRQHLKTSARPDKPVPPDTDIADPQADNLPPAKPFDQIEEW"
BASE COUNT          948 a         1609 c         1632 g         1033 t
ORIGIN      
        1 atgttacgca gcagggcagt cgccctaaaa caaagttaga tgctttgctg tgcgcacaaa
       61 tttcggccag caacaagact gttttttttc ttaaatcgaa cctaaaattt cttcgcggaa
      121 ctccatggag aaatattttg aaaaattggt tatttctggc tacggccatt atttttgagg
      181 tcattgcaac ctctgcgctc aagtctagtg agggctttac taggttagta ccgtctttta
      241 tcgtcgtagc gggatacgct gctgcttttt atttcctgtc gctgacactc aaatcgattc
      301 ctgttggaat cgcctacgca gtttggtcgg gcctcgggat cgtcttggtc actgcgattg
      361 catgggtttt gcatggtcaa aaactagata tgtggggatt tgttggtgtc ggcttcatta
      421 tcagcggcgt tgctgtgctc aacttgctat ctaaggcaag tgttcactaa aacggtcgca
      481 tctaaccatt ccgtcgagag ggaccgccca caagctgcgc ttgcgggttc ccttcgcggc
      541 ttcgccgcta cggcggcccc tcacgtcaaa cgttgaacga cagctttccc aaaagctcta
      601 cggctgctct gggtcgacac cggtaatcgg atcgttgccg cactgaacag cgccccgttc
      661 caggtcgcct ccatttatgc ggctgaaccg agggagagca gctttacgcc gtctggccgc
      721 agttcgccct tgggcgacac gtgccggtag agcgtctgcc gggtaatccc gagttcttcg
      781 cagagatcgc ccaccttggt ttccggttgc cccatgctgg ccatcgccag gcgtagcttg
      841 gcggcggtca tcttgaaggg gcgcccccct ttcctgccgc gagcgcgcgc cgagataagt
      901 ccagcgactg ttcgctcgga aatcaactca cgctcgaact cggccagcgc ggcaaaaata
      961 ccgaacacaa gcttgccggc ggcagtcgtc gtgtcgaccg ccgcaccgtg accggtcagg
     1021 accttcaggc ccacgctacg cgcagttagg tcgtgcacgg tgttgatcag gtggcgcaga
     1081 tcacggccaa gccgatcgag cttccacacg atcagcgtgt ccccttcacg aagcgccttc
     1141 aggcaagcag ccaaccctgg gcgatcatcg cgcctgcccg aggccagatc ctcgtaaagg
     1201 tgcgcaaggc tcacaccagc ggcgatgagc gcatcgcgtt gcaaattggt ggactgggat
     1261 ccgtccgcct tcgatacccg catgtagccg atcagcacct tttcacccgt cacgtatacg
     1321 ttcgattatg tgacagtgtg agccggaaag ctctatccgt caaaatttgt cacttaaccc
     1381 gtcattttgt ttaagacatg caaagggtgc atcaggcagg taatgtgaca gaaattccgg
     1441 cgggatgcct tccttcacga aggtggcgcg caaccgttcc aaggaagcgg gatcgcgccg
     1501 accataggaa gccaacgcga acagcgagcg tgccgtctcg gggttgtgtc gcgcttcaat
     1561 gatggcctcg tcgatggcct ggactgcacg ctgccagtga ttgatgggct cgggcttcgg
     1621 cgctttcgtc ggcaggccac tggtgaaccc ggtttggggc tcggaccaga atagctttgc
     1681 ctgctcgcct ggcgggctga tgtccctcac ctcaatcaag ctgattgccg ttgccgccgc
     1741 ctccagcatc tgtaaccgta ctatcggatt cagggtttca tacggtcgcc acagactttg
     1801 cccagcacgc agcggatgcc cgcagccttc ccagacttgg cggggatacc ccgcgcaggt
     1861 tccgcacgcg gaaagcgggg tgttcagctc atcgagcagc gtgcgaagaa gtcgaaacca
     1921 caatccggcg tggatgcgtc ggcgcggcag ctccacgtga ccggttgtca gtgcctgcca
     1981 ggtacgctgg tccatcgccg caatcgcgtc gctggcggtg cgcggttcgg cgtcggcgtt
     2041 ctcccagccg agaaaccgcc ctggcacgcc ccaataggat tccagccagc agccatgcag
     2101 cgggcagctc agcatcaggg gcagcttcca cgcgagcagt acggcttggt tctccggatc
     2161 gctcaggcag agcggacagg cgcggtttat cggctggctg ggcagccagg cacgccagct
     2221 cgtgatggat cgcgtcttac ggcggagtct tggcagcaac accgagagct gaaacgcata
     2281 ggtttccaag gcgtctggaa tctggtcatc aaggctgtcc agtagccaag gcacccatcc
     2341 ggcgaaactc atacagcgca gccggtccag ctcgatgccg ctccgctggg aaagcaacgc
     2401 cagcagcgag agtggtggcg cggtgtccag gtcatcgacc tggccgtgac caagatcgtg
     2461 ctccagcagg tcgggctcct ccatgtgata gcaaagggcc acgcggttga gccatgagga
     2521 cagcgcctcg ccttctttcg gggcgggatg cagcggccag cgcggcgcag gcttcacatc
     2581 agttcccgct cgaattgccg ccgccgctcg ctgggtccgg tgtaaacggc catgctgagt
     2641 gtgcgatggt tgatcgcttc ctcgccgctc tccacggcga cgatggccgc cgccatcagc
     2701 aagtgcgcca gttcccctat ggtgccctcg ctgcgtgtga gcaggtagcg agccatgtcc
     2761 agcgtggcaa ttggggaagg ccggcgcagc gggagcgaag cggcgaagct ggccagcagt
     2821 gagcagcaat cgtcgttggc ctcccatacc ggcagcatca tcggctcgaa gcgattttcc
     2881 aactggtcat cggagcggat ggctaggtag gcgtcgcgcg tgcctacccc aaccaacggg
     2941 atgcgcagtt cgttgccgag gaagcgcagc aggttgagga attcccggcg gttgacgctg
     3001 ttgccggcca gcacgttgtg cagctcgtcg atcaccagca tgcgcacgcc gaccttgcgc
     3061 agcagtgcca gagccagttg ctccatttcc ggcaaccgtg ggcgtgggcg cagcggcgcg
     3121 cccatcgcgg cgagcagcgc gacgtagaag cggatcacgg acggctcgga cggcatctgc
     3181 acgaccaaca ccgggatgtg ctcctggtcg gcgtcggagc tggccgggtg ggtgcggcgg
     3241 aacttctcga cgatcatcga cttgccattg ttggtcgggc caaccagcag caggttgggc
     3301 atgcgttgct tgtttggcca cgcataaagg gcttccagcc ggttcagcgc ctcgactgcg
     3361 cgcggatagc cgatccagcg gtcggcgcga aggcgctgga tgcgctcgtc cgccggaaga
     3421 cgggccaagc cctgggccgc cggcagcagg tgggacaggt cgatgatggg atattcgtcc
     3481 acggctacca ctcctcaatc tggtcgaacg gtttggcggg tggcaagttg tctgcctgcg
     3541 ggtcggcaat atccgtatcc ggcggaacgg gcttgtccgg ccgagctgat gtcttgaggt
     3601 gctggcggcg atccgcgtca cgccgcgcct tgcgtgtggc cttctgcgcg ctggtcacaa
     3661 tctcacgcat ctggccgatc atgcggaaca gcgccgactc atccacctgt tcgcgccctt
     3721 gctgccgcag tttcgccagc gcctgccgtt gttcccagag ggtgacagcc ggatgcgaca
     3781 aggtacggta gggaatttcc aggtaatgct gtccctccgg ttccaggacc cagatacggc
     3841 tgatgtcgcg cggatcgcgc cggatcagaa aggacggcca gcgttcacgc cgcgcaatcc
     3901 acggcttgag cgcatcggcg tagtagtgga tgtggtcgat gacaaagccg gtgcgggtca
     3961 gcgtgcgccg gaggatcggc agaaaatcga ccaggaacga agtagcgcgt gtgacgacgg
     4021 ccggtacgcc gacacgcgcc acggcctcgg cccagcgcgc ggccggcggt tggagcaggc
     4081 cgttgtgcac cgaaccgtgg taggtgccga ccgccaatgt gagccagcgc tctagctcgc
     4141 gcagcgtcag ggcggccttg ttttcggaat cgtagtcgcc gcgctggtca gggttggaga
     4201 aggtcgttcc cggcagttcg tcgtgaatca tctgcatcgc cgtgccgatg atccgttcca
     4261 cgatgccgcc atagtgcggc tgtcccagcg ggcgatagtc cagccggatg ccatgctgct
     4321 cgcaaccccg gcgcagggcc tcgctcttga actcggccgc gttgtctagg tagagcagca
     4381 agggcttgcc gctcatctgc caatccattt ccacgttcag tccttccagc caagggcgct
     4441 tgtcgcaggc gacatgcacg aggcacaggc caaccgaaac ggcagacggc gcttccagcg
     4501 tgacgaccat gccgagcacg cagcgggtga acacgtcgat ggcgagggtc aggtacgggc
     4561 ggccaatagg ttgccggtcg cggtcatcga ccacgatcag gtcgatgacc gtatggtcta
     4621 tctgcacctg ctccagcggc gcggtcacgg caggaggctc gccgcccaca ccttgtaggt
     4681 cacgagcggc atcctggcct tcccgccggc ggatgacctt gcgcgggtca aggctagcga
     4741 tccgtaaggc cacggtattg cgcgccggca ctcgcagttt ttgagccttg cacacctgag
     4801 tgacttcgcg gtgaaaggcc gctaggctgc gcttctgctt ggtcaggaac cgcttttgca
     4861 gtagctcgtg gatgacgcgc tcgaccggtt ccggcaagcg ccccttacct ttacctccac
     4921 cggactggcc gggcaccaga tccgtcacga ggccgctgcc ttgccgggca cgccggatca
     4981 gaacgtatac ctggcgccga gacaagccca gcgcctgagc cgccatatcg gccgcttcgt
     5041 gcccgaccgt ctccgactgc gccaacggac tgatgatctc cgcacgacgg cgcgcacgct
     5101 cccaagcctc atcaggcaga gtggccacgc cttgttctgg aatccgtggg gtgtccgtcg
     5161 ccatgctcac ctcgctttgg tgcacacgag tattgagcat agtcgagatt ggtgcagatc
     5221 ac
//
LOCUS       DAPGOF010000449         4326 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.guided.268, whole genome shotgun sequence.
ACCESSION   DAPGOF010000449 DAPGOF010000000
VERSION     DAPGOF010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.guided.268"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            226..1371
                     /locus_tag="RHV13_004911"
     CDS             226..1371
                     /locus_tag="RHV13_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C beta-lactamase CMY-2"
                     /protein_id="HDV1244322.1"
                     /translation="MMKKSLCCALLLTASFSTFAAAKTEQQIADIVNRTITPLMQEQA
                     IPGMAVAVIYQGKPYYFTWGKADIANNHPVTQQTLFELGSVSKTFNGVLGGDAIARGE
                     IKLSDPVTKYWPELTGKQWQGIRLLHLATYTAGGLPLQIPDDVRDKAALLHFYQNWQP
                     QWTPGAKRLYANSSIGLFGALAVKPSGMSYEEAMTRRVLQPLKLAHTWITVPQNEQKD
                     YAWGYREGKPVHVSPGQLDAEAYGVKSSVIDMARWVQANMDASHVQEKTLQQGIALAQ
                     SRYWRIGDMYQGLGWEMLNWPLKADSIINGSDSKVALAALPAVEVNPPAPAVKASWVH
                     KTGSTGGFGSYVAFVPEKNLGIVMLANKSYPNPVRVEAAWRILEKLQ"
     gene            1465..1998
                     /locus_tag="RHV13_004912"
     CDS             1465..1998
                     /locus_tag="RHV13_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HDV1244323.1"
                     /translation="MRILPVVAAVTAAFLVVACSSPTPPKGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLDKVTATYSLRDDGGINVINKGYNPDREMWQKTEGKAYFTGDPSRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEMKQQMLAIAT
                     REGFEVNKLIWVKQPGA"
     gene            complement(1995..2312)
                     /gene="sugE"
                     /locus_tag="RHV13_004913"
     CDS             complement(1995..2312)
                     /gene="sugE"
                     /locus_tag="RHV13_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDV1244324.1"
                     /translation="MSWIVLLIAGLLEVVWAIGLKYTHGFTRLTPSIITIAAMIVSIA
                     MLSWAMRTLPVGTAYAVWTGIGAVGAAITGILLLGESASPARLLSLGLIVAGIIGLKL
                     STH"
     gene            3040..>4326
                     /locus_tag="RHV13_004914"
     CDS             3040..>4326
                     /locus_tag="RHV13_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4165 domain-containing protein"
                     /protein_id="HDV1244325.1"
                     /translation="MINFKPKIPAMLGALAVLTAGAAHAELLEYTFKAPDGTQRSLTP
                     NANYANPTGNISFALSAGIDRKVKISVLRSDGTVVSTATSHLLGATDRITVGGKSYYG
                     AELQLPAPVGGAYTIRAEILASDGSTVQTDEYPLTVDVTPPTYSSLAPVYSNYGQVTS
                     GDVWKLGLGGSEDNAFLLSGISDESPIKGVKAKLYRQDGSLYKDVSVNYDDANGQARQ
                     SFQSGFFPASDLDEVFTLQFEISDSAGNSYLSPRQKVMFDSITNAPSAPFGVYDPSST
                     NNLGPGLTGFVAYTEGMTVKTNPIKLAWRVPRDNWHEYREGGINMTNALGEMSKVGED
                     ASYVYLVTTAPYGNTDGNYWRWVNFGQWGGGGIAYNLTLSPSAPKSPRLLGVDYNYSD
                     IGWSSFYRYWVNNSVLPVTVSSIRVKVEPRPYVQTAV"
BASE COUNT         1119 a         1081 c         1055 g         1071 t
ORIGIN      
        1 aatgattgaa aggtggttgt aaataatgtt acaatgtgtg agaagcagtc taaattcttc
       61 gtgaaatagt gatttttgaa gctaataaaa aacacacgtg gaatttagga aaaacttata
      121 tctgctgcta aatttaaccg tttgtcaaca cggtgcaaat caaacacact gattgcgtct
      181 gacgggcccg gacacctttt tgcttttaat tacggaactg atttcatgat gaaaaaatcg
      241 ttatgctgcg ctctgctgct gacagcctct ttctccacat ttgctgccgc aaaaacagaa
      301 caacagattg ccgatatcgt taatcgcacc atcaccccgt tgatgcagga gcaggctatt
      361 ccgggtatgg ccgttgccgt tatctaccag ggaaaaccct attatttcac ctggggtaaa
      421 gccgatatcg ccaataacca cccagtcacg cagcaaacgc tgtttgagct aggatcggtt
      481 agtaagacgt ttaacggcgt gttgggcggc gatgctatcg cccgcggcga aattaagctc
      541 agcgatccgg tcacgaaata ctggccagaa ctgacaggca aacagtggca gggtatccgc
      601 ctgctgcact tagccaccta tacggcaggc ggcctaccgc tgcagatccc cgatgacgtt
      661 agggataaag ccgcattact gcatttttat caaaactggc agccgcaatg gactccgggc
      721 gctaagcgac tttacgctaa ctccagcatt ggtctgtttg gcgcgctggc ggtgaaaccc
      781 tcaggaatga gttacgaaga ggcaatgacc agacgcgtcc tgcaaccatt aaaactggcg
      841 catacctgga ttacggttcc gcagaacgaa caaaaagatt atgcctgggg ctatcgcgaa
      901 gggaagcccg tacacgtttc tccgggacaa cttgacgccg aagcctatgg cgtgaaatcc
      961 agcgttattg atatggcccg ctgggttcag gccaacatgg atgccagcca cgttcaggag
     1021 aaaacgctcc agcagggcat tgcgcttgcg cagtctcgct actggcgtat tggcgatatg
     1081 taccagggat taggctggga gatgctgaac tggccgctga aagctgattc gatcatcaac
     1141 ggcagcgaca gcaaagtggc attggcagcg cttcccgccg ttgaggtaaa cccgcccgcc
     1201 cccgcagtga aagcctcatg ggtgcataaa acgggctcca ctggtggatt tggcagctac
     1261 gtagccttcg ttccagaaaa aaaccttggc atcgtgatgc tggcaaacaa aagctatcct
     1321 aaccctgtcc gtgtcgaggc ggcctggcgc attcttgaaa agctgcaata actgacgatg
     1381 aggcccagga tattgggcct cctttctttc tctttttttc ctgttgtcat ctacacttaa
     1441 caaaaataca gcaaggaaaa tcccatgcgc attttgcccg tcgttgctgc agttacggct
     1501 gcattcctgg ttgtcgcgtg tagctccccg acaccgccga aaggcgttac cgtggtaaat
     1561 aactttgatg ccaaacgcta tctgggaacc tggtatgaaa ttgcgcgctt cgaccatcgt
     1621 ttcgagcgcg gattggataa agtgaccgca acatacagct tgcgcgacga cggcggcatc
     1681 aacgttatta acaagggcta taaccctgac agggagatgt ggcagaaaac ggaagggaaa
     1741 gcctatttca ccggcgaccc aagcagagcc gcgcttaagg tttctttttt cggccccttc
     1801 tatggcgggt ataacgtaat tgcactcgac cgggaatatc gtcacgcgct ggtttgtggt
     1861 ccggatcgcg actacctgtg gatcctttca cggaccccta ctatttcaga tgaaatgaaa
     1921 cagcaaatgt tagccatcgc gacccgggaa gggtttgaag tgaataaact gatttgggtg
     1981 aaacagcctg gcgcttagtg agtgctcagc ttcagaccaa taatgccagc aacgatcagc
     2041 ccaaggctca gcaaacgtgc cgggctggca gactcaccca gcagcaaaat ccctgtaatg
     2101 gccgccccaa cagcgccaat accggtccag accgcataag cggttcctac aggcaacgtg
     2161 cgcattgccc aagagagcat ggcgatactg acgatcatcg ccgcaatagt gataatgctt
     2221 ggcgtaagac gcgtaaaacc gtgggtgtat ttcaggccaa tcgcccagac aacttcgagc
     2281 aaacctgcaa ttaataaaac gatccaggac atatcaggct ccagaacaat ggggccgtcc
     2341 ccggtgaaag aagcgtttgc aggtcgtcct gcaaagctaa tgtgtgaaat ggcatttttg
     2401 cccggaagaa aatgaatttc aaccttttta ttcaccgcct gctaaaagca agaattaagc
     2461 ataattagcg gcgtagttcc cgcatttggc actcatcgga gttctctatg atgaagggct
     2521 ttctgaaacc agaaagtcga ttgcccctct tctgaatgcg aaacgtttat gcttaaaatt
     2581 ttagtgattg accggtgcca ctttacccgc acagggatag aggccttgct taatcattct
     2641 ggcaggttca gctcctcatt tctggtatca ggaatcaata atctcctgtt agcaaaagag
     2701 catattttgc aatggaaacc gcatctggtg atcgcggatt tatacagttt tattagcgag
     2761 acgcactcca gtccgccaat taaacctttt tttatgagtt gtggcgtcat cccactaatt
     2821 ttactgcaat cagcagacag acagcatgca cccatcgtgc cctctcaatc cgtagcccat
     2881 tcggtcttga ccaagcacac cacgctgaac acactctccc acacaattca ggatttcctt
     2941 atttgctcaa aaagggcact tccacagccc atgtttatgc actccctccc gtaacctcca
     3001 gcattgggtt cttgtaactc aaaacgaatg gagaatgcga tgatcaattt caagcctaaa
     3061 attccggcga tgcttggggc tctggcggtt cttaccgctg gtgctgctca tgcagagctg
     3121 ctggaataca cctttaaggc tccagatggt acgcaacggt ctctgactcc gaatgccaac
     3181 tatgccaacc caacgggcaa tatctcgttt gccttgagtg ccggtatcga ccgaaaggta
     3241 aagatttcgg tgcttcggtc ggatggaaca gtggtttcga cagcgaccag ccaccttctt
     3301 ggggctactg atcgcatcac agtgggtgga aaatcctact atggtgcgga acttcaacta
     3361 ccagctccag ttggtggggc atacactatc agggcggaaa ttcttgcctc tgatggttct
     3421 acagtacaga cggatgaata ccctcttact gtcgatgtga cgccgccaac ctactcttcg
     3481 cttgccccgg tttatagtaa ctatgggcag gttactagcg gtgatgtttg gaaactgggt
     3541 ctaggtggct ctgaggataa tgcctttttg ctgtcaggaa tctctgatga gtcacccatc
     3601 aaaggcgtca aagcaaagct gtatcgtcaa gacggttcac tctacaaaga tgtatccgtg
     3661 aactacgatg acgctaacgg acaagctaga cagtctttcc aatctggctt tttcccagcc
     3721 tctgacttgg atgaagtgtt tacacttcag tttgagataa gcgatagcgc tggaaatagc
     3781 tatctttcac ctcgtcaaaa agtgatgttt gatagcataa ccaatgctcc gtctgcacct
     3841 tttggtgttt atgacccttc aagtacaaac aacttaggcc ctggattaac cggatttgtt
     3901 gcttacactg agggcatgac agttaaaact aacccaatca agttagcttg gcgtgttccg
     3961 cgtgataact ggcatgagta tagagaaggt gggataaata tgaccaatgc cttgggagag
     4021 atgtccaagg taggagaaga tgccagctat gtttatctcg tcacaaccgc accttatggc
     4081 aataccgacg gcaattattg gcgttgggtg aacttcggcc agtggggggg aggcggtatt
     4141 gcttataacc tcacactctc tccatcagcg cctaaaagtc ccagactttt gggtgtggac
     4201 tacaattaca gtgatatagg atggtctagt ttttaccgct actgggtgaa caattctgtt
     4261 cttcctgtca ctgttagtag tatccgtgtc aaagttgaac caaggcctta cgttcaaact
     4321 gccgtt
//
LOCUS       DAPGOF010000450         3052 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.guided.294, whole genome shotgun sequence.
ACCESSION   DAPGOF010000450 DAPGOF010000000
VERSION     DAPGOF010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.guided.294"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            28..413
                     /locus_tag="RHV13_004915"
                     /pseudo
     CDS             28..413
                     /locus_tag="RHV13_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
     gene            421..1107
                     /locus_tag="RHV13_004916"
     CDS             421..1107
                     /locus_tag="RHV13_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDV1244326.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1085..1708)
                     /gene="tetR(B)"
                     /locus_tag="RHV13_004917"
     CDS             complement(1085..1708)
                     /gene="tetR(B)"
                     /locus_tag="RHV13_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HDV1244327.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1790..2995
                     /gene="tet(B)"
                     /locus_tag="RHV13_004918"
     CDS             1790..2995
                     /gene="tet(B)"
                     /locus_tag="RHV13_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HDV1244328.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
BASE COUNT          815 a          573 c          688 g          976 t
ORIGIN      
        1 aatattcatt taaaaaggac aaggataatg tttgatgttc acgttgtttt agataatcaa
       61 ataggacaat tagcattact aggaaaaaca ttaggtaata aaggtatggg ttggaagggg
      121 gagggatatt tacggttggt gatgaatgcc atgctcattt tcttgttgaa caaggaaagg
      181 aagctaaaat agcgctagag caagctggac tgttagtact tgcgatccgg acaccattaa
      241 ttcgtaagtt aaaacaggaa aaaccggggg aacttggcga aatagcacga gtattggcgg
      301 agaataacat taatatttta gtgcaataca gtgaccatgc taaccaactg atattaataa
      361 cggacaatga tagtatggct gcatctgtta cgctcccttg ggcaataaag tgaacttgcg
      421 atggctaatt taatacgaaa agaggttacc tttgagtcct caatagccgc gataggggcg
      481 gctatgtctg acatttcacg agttaaaata ctcagtgctt tgatggatgg gcgagcttgg
      541 acggccactg agctaagttc tgtggcgaat atatcagctt caacggcgag cagtcattta
      601 tctaaattat tagattgcca gctaatcaca gtagtagctc aaggcaagca tcgttatttt
      661 cggctagcag gaaaagatat tgctgaattg atggaaagta tgatggggat ctccttaaac
      721 catggcgtac atgccaaagt ttccacgcca gtgcatttac gaaaagcacg tacttgctat
      781 gatcatttag ctggcgaagt tgccgttaag atctatgatt ccctttgtca acagcaatgg
      841 atcactgaaa atggttcaat gatcacatta agtggtattc aatattttca tgaaatggga
      901 attgacgttc cttccaaaca ttcacgtaaa atctgttgtg cgtgtttaga ttggagtgaa
      961 cgccgtttcc atttaggtgg gtacgttgga gccgcattat tttcgcttta tgaatctaaa
     1021 gggtggttaa ctcgacatct tggttaccgt gaagttacca tcacggaaaa aggttatgct
     1081 gcttttaaga cccactttca catttaagtt gtttttctaa tccgcatatg atcaattcaa
     1141 ggccgaataa gaaggctggc tctgcacctt ggtgatcaaa taattcgata gcttgtcgta
     1201 ataatggcgg catactatca gtagtaggtg tttccctttc ttctttagcg acttgatgct
     1261 cttgatcttc caatacgcaa cctaaagtaa aatgccccac agcgctgagt gcatataatg
     1321 cattctctag tgaaaaacct tgttggcata aaaaggctaa ttgattttcg agagtttcat
     1381 actgtttttc tgtaggccgt gtacctaaat gtacttttgc tccatcgcga tgacttagta
     1441 aagcacatct aaaactttta gcgttattac gtaaaaaatc ttgccagctt tccccttcta
     1501 aagggcaaaa gtgagtatgg tgcctatcta acatctcaat ggctaaggcg tcgagcaaag
     1561 cccgcttatt ttttacatgc caatacaatg taggctgctc tacacctagc ttctgggcga
     1621 gtttacgggt tgttaaacct tcgattccga cctcattaag cagctctaat gcgctgttaa
     1681 tcactttact tttatctaat ctagacatca ttaattccta atttttgttg acactctatc
     1741 attgatagag ttattttacc actccctatc agtgatagag aaaagtgaaa tgaatagttc
     1801 gacaaagatc gcattggtaa ttacgttact cgatgccatg gggattggcc ttatcatgcc
     1861 agtcttgcca acgttattac gtgaatttat tgcttcggaa gatatcgcta accactttgg
     1921 cgtattgctt gcactttatg cgttaatgca ggttatcttt gctccttggc ttggaaaaat
     1981 gtctgaccga tttggtcggc gcccagtgct gttgttgtca ttaataggcg catcgctgga
     2041 ttacttattg ctggcttttt caagtgcgct ttggatgctg tatttaggcc gtttgctttc
     2101 agggatcaca ggagctactg gggctgtcgc ggcatcggtc attgccgata ccacctcagc
     2161 ttctcaacgc gtgaagtggt tcggttggtt aggggcaagt tttgggcttg gtttaatagc
     2221 ggggcctatt attggtggtt ttgcaggaga gatttcaccg catagtccct tttttatcgc
     2281 tgcgttgcta aatattgtcg ctttccttgt ggttatgttt tggttccgtg aaaccaaaaa
     2341 tacacgtgat aatacagata ccgaagtagg ggttgagacg caatcgaatt cggtatacat
     2401 cactttattt aaaacgatgc ccattttgtt gattatttat ttttcagcgc aattgatagg
     2461 ccaaattccc gcaacggtgt gggtgctatt taccgaaaat cgttttggat ggaatagcat
     2521 gatggttggc ttttcattag cgggtcttgg tcttttacac tcagtattcc aagcctttgt
     2581 ggcaggaaga atagccacta aatggggcga aaaaacggca gtactgctcg gatttattgc
     2641 agatagtagt gcatttgcct ttttagcgtt tatatctgaa ggttggttag ttttccctgt
     2701 tttaatttta ttggctggtg gtgggatcgc tttacctgca ttacagggag tgatgtctat
     2761 ccaaacaaag agtcatcagc aaggtgcttt acagggatta ttggtgagcc ttaccaatgc
     2821 aaccggtgtt attggcccat tactgtttgc tgttatttat aatcattcac taccaatttg
     2881 ggatggctgg atttggatta ttggtttagc gttttactgt attattatcc tgctatcgat
     2941 gaccttcatg ttaacccctc aagctcaggg gagtaaacag gagacaagtg cttagttatt
     3001 tcgtcaccaa atgatgttat tccgcgaaat ataatgaccc tcttgataac cc
//
LOCUS       DAPGOF010000451         2206 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.guided.320, whole genome shotgun sequence.
ACCESSION   DAPGOF010000451 DAPGOF010000000
VERSION     DAPGOF010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.guided.320"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            37..384
                     /locus_tag="RHV13_004919"
     CDS             37..384
                     /locus_tag="RHV13_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HDV1244329.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            378..1217
                     /gene="sul1"
                     /locus_tag="RHV13_004920"
     CDS             378..1217
                     /gene="sul1"
                     /locus_tag="RHV13_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HDV1244330.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPEDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            1344..1844
                     /locus_tag="RHV13_004921"
     CDS             1344..1844
                     /locus_tag="RHV13_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV1244331.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLVASCSDGIVGCCTLSAEDPEFWPDALKGEAAYLHKLAVRRTHA
                     GRGVSSALIEACRHAARTQGCAKLRLDCHPNLRGLYERLGFTHVDTFNPGWDPTFIAE
                     RLELEI"
BASE COUNT          404 a          661 c          662 g          479 t
ORIGIN      
        1 tgcataataa gccctacaca aattgggaga tatatcatga aaggctggct ttttcttgtt
       61 atcgcaatag ttggcgaagt aatcgcaaca tccgcattaa aatctagcga gggctttact
      121 aagcttgccc cttccgccgt tgtcataatc ggttatggca tcgcatttta ttttctttct
      181 ctggttctga aatccatccc tgtcggtgtt gcttatgcag tctggtcggg actcggcgtc
      241 gtcataatta cagccattgc ctggttgctt catgggcaaa agcttgatgc gtggggcttt
      301 gtaggtatgg ggctcataat tgctgccttt ttgctcgccc gatccccatc gtggaagtcg
      361 ctgcggaggc cgacgccatg gtgacggtgt tcggcattct gaatctcacc gaggactcct
      421 tcttcgatga gagccggcgg ctagaccccg ccggcgctgt caccgcggcg atcgaaatgc
      481 tgcgagtcgg atcagacgtc gtggatgtcg gaccggccgc cagccatccg gacgcgaggc
      541 ctgtatcgcc ggccgatgag atcagacgta ttgcgccgct cttagacgcc ctgtccgatc
      601 agatgcaccg tgtttcaatc gacagcttcc aaccggaaac ccagcgctat gcgctcaagc
      661 gcggcgtggg ctacctgaac gatatccaag gatttcctga ccctgcgctc tatcccgata
      721 ttgctgaggc ggactgcagg ctggtggtta tgcactcagc gcagcgggat ggcatcgcca
      781 cccgcaccgg tcaccttcga cccgaagacg cgctcgacga gattgtgcgg ttcttcgagg
      841 cgcgggtttc cgccttgcga cggagcgggg tcgctgccga ccggctcatc ctcgatccgg
      901 ggatgggatt tttcttgagc cccgcaccgg aaacatcgct gcacgtgctg tcgaaccttc
      961 aaaagctgaa gtcggcgttg gggcttccgc tattggtctc ggtgtcgcgg aaatccttct
     1021 tgggcgccac cgttggcctt cctgtaaagg atctgggtcc agcgagcctt gcggcggaac
     1081 ttcacgcgat cggcaatggc gctgactacg tccgcaccca cgcgcctgaa gatctgcgaa
     1141 gcgcaatcac cttctcggaa accctcgcga aatttcgcag tcgcgacgcc agagaccgag
     1201 ggttagatca tgcctagcat tcaccttccg ccgcccgcta gcggaccctg gtcaggttcc
     1261 gcgaaggtgg gcgcagacat gctgggctcg tcaggatcaa actgcactat gaggcggcgg
     1321 ttcataccgc gccaggggag cgaatggaca gcgaggagcc tccgaacgtt cgggtcgcct
     1381 gctcgggtga tatcgacgag gttgtgcggc tgatgcacga cgctgcggcg tggatgtccg
     1441 ccaagggaac gcccgcctgg gacgtcgcgc ggatcgaccg gacattcgcg gagaccttcg
     1501 tcctgagatc cgagctccta gtcgcgagtt gcagcgacgg catcgtcggc tgttgcacct
     1561 tgtcggccga ggatcccgag ttctggcccg acgccctcaa gggggaggcc gcatatctgc
     1621 acaagctcgc ggtgcgacgg acacatgcgg gccggggtgt cagctccgcg ctgatcgagg
     1681 cttgccgcca tgccgcgcga acgcaggggt gcgccaagct gcggctcgac tgccacccga
     1741 acctgcgtgg cctatacgag cggctcggat tcacccacgt cgacactttc aatcccggct
     1801 gggatccaac cttcatcgca gaacgcctag aactcgaaat ctaacgtccg ttcgggcatc
     1861 gaggtccatg tcggggtggg acgggcccgt ggcttcaaga tcacttgcag tccgaccgcg
     1921 atgtcttggt tgcgcgagag gttgtcgata tcctccactt ccatcatcaa ccctggataa
     1981 tgccgccgcc gtcatcgccg ccgacgcccg tgccgggctt ttcgggcctg tcaggcttgc
     2041 tcggccttca gcctgcctgg gcgagatctc cggcggacgg attaacggcg gagcttcgcc
     2101 gcctttcgtg cgtgtgaagg ccgaagatag ttctctcaaa aacatccgtt tatgagagat
     2161 accaaatgtc attttcagaa gacgactgca ccagttgatt gggcgt
//
LOCUS       DAPGOF010000452         1982 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.guided.326, whole genome shotgun sequence.
ACCESSION   DAPGOF010000452 DAPGOF010000000
VERSION     DAPGOF010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.guided.326"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            106..921
                     /locus_tag="RHV13_004922"
     CDS             106..921
                     /locus_tag="RHV13_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(3')-I family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HDV1244332.1"
                     /translation="MSHIQRETSCSRPRLNSNLDADLYGYRWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTAFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAAFLRRLHSIPVCNCPFNSDRAFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            complement(1117..1881)
                     /locus_tag="RHV13_004923"
     CDS             complement(1117..1881)
                     /locus_tag="RHV13_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HDV1244333.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
BASE COUNT          452 a          516 c          503 g          511 t
ORIGIN      
        1 atgataagtt tatcaccacc gactatttgc aacagtgccg ataaaaatat atcatcatga
       61 acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa
      121 cgggaaacgt cttgctcgag gccgcgatta aattccaacc tggatgctga tttatatggg
      181 tatagatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg attgtatggg
      241 aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt
      301 acagatgaga tggtcagact aaactggctg acggcattta tgcctcttcc gaccatcaag
      361 cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc cgggaaaaca
      421 gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca
      481 gcgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc
      541 gcatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat
      601 tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataagctt
      661 ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt
      721 tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga
      781 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa
      841 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg
      901 atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt
      961 acgctgactt gacgggacgg cgcttcccta ccctcatggc cgcctcccag gaatcgaccc
     1021 aggtgcttat cgtggacata atcctttact cataaattta tcggctctgt tgcaaagatt
     1081 ggcggcagtc agaggtaggc tgtcgctctg cgccgatcag gcggctgctg cgaaatggtg
     1141 gttgagcatg cccatggcct ccgtcagcgc cgagggccca atgccaaaag ctctctccac
     1201 aaggcgcacc tcgcccctga tgccgggctg caggcaccag gggcgagcct gtcctttgcg
     1261 cagggctcgc atgacttcga atcccttgat cgtggcatag gccgtgggga tcgatttgaa
     1321 accgcgcacc ggcttgatca gtatcttgag ctttccgtga tcggcctcga tcacgttatt
     1381 gagatacttc acctgccggt gggccgtctc ccggtccagc tttccttcgc gcttcaattc
     1441 ggtgatcgct gcaccatagc tcggcgcttt gtcggtattg agcgtggcag gcttttccca
     1501 gtgcttcagg cctcgcaggg ccttgcccag gaaccgcttc gctgccttgg cgctgcgggt
     1561 cggcgacagg tagaaatcga tcgtgtcgcc ccgcttgtcg actgcccggt acaggtaggt
     1621 ccacttgccc cgcaccttga cgtaggtttc atccaggcgc cagctcggat caaagccacg
     1681 ccgccagaac cagcgcagcc gcttctccat ctccggggcg tagcactgga cccagcgata
     1741 gatcgtcgta tggtcgaccg aaatgccgcg ttccgccagc atttcctcaa ggtcgcgata
     1801 gctgatcgga tagcgacaat accagcgcac cgcccacagg atcacatcac cctggaaatg
     1861 gcgccacttg aaatccgtca tcgttccgtc cgtccaatct ccgccaagca tgctcaagct
     1921 tcacgatttt tgcaacagag cccacacgag tattgagcat agtcgagatt ggtgcagatc
     1981 ac
//
LOCUS       DAPGOF010000453         1032 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.guided.381, whole genome shotgun sequence.
ACCESSION   DAPGOF010000453 DAPGOF010000000
VERSION     DAPGOF010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.guided.381"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>1032
                     /locus_tag="RHV13_004924"
     CDS             <1..>1032
                     /locus_tag="RHV13_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein"
                     /protein_id="HDV1244334.1"
                     /translation="SINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLPQRKKIE
                     NPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQMEV
                     ISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLPLGS
                     GMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVSTPA
                     DFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIVDTQLKN
                     NEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPNSRIDK
                     VRYMFNKIT"
BASE COUNT          332 a          175 c          213 g          312 t
ORIGIN      
        1 cttcaataaa tggtgaatta tgtaaaatcg ataaggctta ttccggggaa attgttattt
       61 tgcaaaatga gtttttgaag ttaaatagtg ttcttggaga tacaaaacta ttgccacaga
      121 gaaaaaagat tgaaaatccg caccctctac tacaaacaac tgttgaaccg agtaaacctg
      181 aacagagaga aatgttgctt gatgcccttt tggaaatctc agatagtgat ccgcttctac
      241 gatattacgt ggattctacg acacatgaaa ttatactttc tttcttaggg aaagtacaaa
      301 tggaagtgat tagtgcactg ttgcaagaaa agtatcatgt ggagatagaa ctaaaagagc
      361 ctacagtcat ttatatggag agaccgttaa aaaatgcaga atataccatt cacatcgaag
      421 tgccgccaaa tcctttctgg gcttccattg gtttatctgt atcaccgctt ccgttgggaa
      481 gtggaatgca gtatgagagc tcggtttctc ttggatactt aaatcaatca tttcaaaatg
      541 cagttatgga agggatacgc tatggctgtg aacaaggatt gtatggttgg aatgtgacgg
      601 actgtaaaat ctgttttaag tatggcttat actatagccc tgttagtacc ccagcagatt
      661 ttcggatgct tgctcctatt gtattggaac aagtcttaaa aaaagctgga acagaattgt
      721 tagagccata tcttagtttt aaaatttatg cgccacagga atatctttca cgagcataca
      781 acgatgctcc taaatattgt gcgaacatcg tagacactca attgaaaaat aatgaggtca
      841 ttcttagtgg agaaatccct gctcggtgta ttcaagaata tcgtagtgat ttaactttct
      901 ttacaaatgg acgtagtgtt tgtttaacag agttaaaagg gtaccatgtt actaccggtg
      961 aacctgtttg ccagccccgt cgtccaaata gtcggataga taaagtacga tatatgttca
     1021 ataaaataac tt
//
LOCUS       DAPGOF010000454          930 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16243
            SAMN37211161-rid19354783.guided.392, whole genome shotgun sequence.
ACCESSION   DAPGOF010000454 DAPGOF010000000
VERSION     DAPGOF010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211161
            Sequence Read Archive: SRR25825187
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875911.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875911.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:31:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 58 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 13 of 80
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211161-rid19354783.guided.392"
                     /isolate="ARS-CC16243"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            45..836
                     /locus_tag="RHV13_004925"
     CDS             45..836
                     /locus_tag="RHV13_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA25"
                     /protein_id="HDV1244335.1"
                     /translation="MRVAVTIEISNQLSEVLSVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          238 a          234 c          250 g          208 t
ORIGIN      
        1 atgttacgca gcagggcagt cgccctaaaa caaagttaga catcatgagg gtagcggtga
       61 ccatcgaaat ttcgaaccaa ctatcagagg tgctaagcgt cattgagcgc catctcgaac
      121 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca
      181 gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt
      241 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg
      301 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg
      361 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca
      421 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg
      481 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc
      541 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg
      601 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg
      661 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg
      721 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcccgc gcagatcagt
      781 tggaagaatt tgttcactac gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc
      841 taacaattcg ttcaagccga cgccgcttcg cggcgcggct taactcaagc gttagatgca
      901 ctaagcacat aattgctcac agccaaacta
//
